LOCUS       DAOSWW010000001        11561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_3538_length_11561_cov_3.604033, whole genome shotgun sequence.
ACCESSION   DAOSWW010000001 DAOSWW010000000
VERSION     DAOSWW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11561
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3538_length_11561_cov_3.604033"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PK442_00005"
     CDS             <1..327
                     /locus_tag="PK442_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HOO86062.1"
                     /translation="IGQRASTCPLGRDVKLNGYPIRVSELLAALPAPGYVTRVTVAQP
                     KYIIKAKQAVKQAFQNQLDGKGFSLVEILSSCPTNWGMRPVEAFDWLVNTMMPYYPLG
                     TFKDFD"
     gene            340..897
                     /locus_tag="PK442_00010"
     CDS             340..897
                     /locus_tag="PK442_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO86063.1"
                     /translation="MSFFKTLLAAGFGGQGVMVLGQLVAYTGIEEGRHVTWIPSYGPE
                     MRGGTANCGVVLSDDEIGSPVVDKADVLVVMNQPSLSKFIDRLKPGGVLVYNSNLVEY
                     DNSRKDIRVVALPATTLAEELGSDKITNVVILGAVVESSGIVADAVCVETIKEKLGKK
                     KPQFLPMNLGAYEKGKEVARECLKA"
     gene            882..1427
                     /locus_tag="PK442_00015"
     CDS             882..1427
                     /locus_tag="PK442_00015"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOO86064.1"
                     /translation="MSEGVREKTLAQKTVYSGRILNLRVDDVELPGGKRAIREVVEHK
                     PAVGILPVMEDGTVLLIRQYRYAVGEDMLEIPAGIVEGNEPFDDAARRELQEEVGYFP
                     EHLREIGRIYNSPGFSTELMALFVATGLRPSKLQEDDDEFITVVPVAKEEIPALITGG
                     KLKDAKTFAALSWLMAAGEVH"
     gene            1530..2423
                     /locus_tag="PK442_00020"
     CDS             1530..2423
                     /locus_tag="PK442_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOO86065.1"
                     /translation="MTLYDFESAHESFLAWLLLERGLSRNTASAYSRDLHKWFAYCTT
                     NGILPFPPDEDGIARFQRFLVESEGSKASRQRTMAGLRTWMRFLQVEEVLEADVALPP
                     LPQREQRLPKILSEGEVDRLLNVCGGDDPLSQRDRALFEVAYGCGLRASEICSLRVGD
                     VDFAAKTLRTLGKSEKERAVPFLGEPAHRVRRYLEEARPRLAKEETGILFLSRNGMML
                     NREDIWRIIQKRGAEAGISKQRLHPHILRHSFATHLLRRGMDQKTLQELLGHSSIATT
                     DKYLHFDLELRDVYDASHPRA"
     gene            2456..3271
                     /locus_tag="PK442_00025"
     CDS             2456..3271
                     /locus_tag="PK442_00025"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOO86066.1"
                     /translation="MIEKVKAASAFLKERISSSPESAVILGSGLGSFADSLEGRTVIP
                     YEEIPHWPLSTAPGHGGRLVHGRLGDRELLVMQGRVHYYEGYPMEEVVFPVRVLAQLG
                     VKNLVITNASGGVDVSLSPGDIVLLEDHVNFMGANPLRGPDTPGWNDRFPDMTRCYDE
                     KFIAAFEAAGAAEGAPVRRGVYFAFSGPSFETPAEIRMARALGATVVGMSTVPEAITA
                     NAMGLRVCALSCVANAAAGLTMNRLSGQEVLDEMAKTSEKLIRLLNRFFKEMR"
     gene            3273..4589
                     /locus_tag="PK442_00030"
     CDS             3273..4589
                     /locus_tag="PK442_00030"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOO86067.1"
                     /translation="MFDMISFIERKRDGGAHTAEEIQEFVRGFASGTVPDYQASAWLM
                     AVFHNGLKGEELKEFTLALAHSGKILSFGDLRIVDKHSTGGVGDKATLLLVPLAASCG
                     VKVAKLSGPGLSFTGGTVDKLEAIPGFQMHLSSERFIEQVRRIGCAISGHSDDLAPAE
                     GKFYALRDVTGTVPTLPLLCSSIVSKKIAGGAHAFVFDVKCGAGAFMEDYENAEKLAR
                     ALVSLSKSLGKESMALITDMDQPLGEWIGNSTEVLEAVSVLQGKGPKDVEELVINLAG
                     AMVSIDSDLSFEEGCALAREKLEDGSGLEAFAAMVRAQGGDDRVCRTPEKILPLAGKK
                     HTITASEDSTVRALNARKIGEGVKRLGGGRMSLGEPIDLSVGVRILKKNGASAGRGEA
                     LLEILYSDEAKLADAMPFFESAYTFGKANEHSEDTAQRRLILGKIG"
     gene            4573..5517
                     /locus_tag="PK442_00035"
     CDS             4573..5517
                     /locus_tag="PK442_00035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOO86068.1"
                     /translation="MGRSGRFTFFRFGSILFLLFCLVCIPLSASAEVTVQYPSSVGVG
                     KPFLVRVTSSVPLTGVEAVWQGRTVQLGVSVWNERYISLALLGTQVGKVKTGDHRLTL
                     RLLSGGKRSEHPFSIKVTPVQYKEDRLTLPEKMVTPPSSELKRIEAERKRTGAALATM
                     TAGRQWGLPIIRPVSGIVTSPYGRRRILNGKPRSPHAGVDFRAAVGTPVLAALPGKVV
                     LTGDHYYAGKSIYIDSGGGVISHYFHMHTIDVKKGDPVKPGQVIGKSGVTGRATGPHL
                     HFGLALAGQMVNPEPLFSATSSGMLEKTALRKVTPRWK"
     gene            5572..6267
                     /locus_tag="PK442_00040"
     CDS             5572..6267
                     /locus_tag="PK442_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86069.1"
                     /translation="MRGWKRAFGSCALLLFFASAGWASSLIADYEARFTKYFTDRIQA
                     RSVLSVIKASEDELRRGKIPTLNLCIEDAVVEGVRYDRLLLVMHDVLFTSAARGIVVH
                     SHGESHLSGGISKETFLKTLSEGMPHFAVSELALRNGSVMIKGVYERRLTFKVRALMR
                     FTGSYVIDGDGVARVRFDDASNDNPAISTLDVAKAMANAASVLSFRDFFGAPLVREVL
                     VDHDMVWFSARNQ"
     gene            6338..6435
                     /locus_tag="PK442_00045"
     tRNA            6338..6435
                     /locus_tag="PK442_00045"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6372..6374,aa:Sec,seq:tca)
     gene            6528..7178
                     /locus_tag="PK442_00050"
     CDS             6528..7178
                     /locus_tag="PK442_00050"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOO86070.1"
                     /translation="MIPWEKRAEEMVEKQIRARGILSERVLEAMRSVPRHLFLPEHLR
                     ESAWIDSPLPIGEEQTMSQPYMAAKMTELLAPEEGDSVLEIGTGSGYQSAVLCSMGAK
                     VTSIERIAPLAERARETLSSLGYDVSVLWSDGREKGELAGEFQCVIVTAAAPALEAWW
                     SEALAPGGRLVAPVTVLSGSERLLLREKKAPGEYVDTWHDYCRFVPLLQGRTGGYC"
     gene            7191..7706
                     /locus_tag="PK442_00055"
     CDS             7191..7706
                     /locus_tag="PK442_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOO86071.1"
                     /translation="MKVSFFKGASPKELFSVRSLALSGLVAALYAVTTILFAPISYGY
                     VQVRISEALTVLPFLFPQAVFGLFVGCLFANLFGGFGILDVVLGSAATLLAGIVSSAM
                     PTVYLAALPPVLVNMVIVGGYLSFILDLPFPLCAMYVGIGQFIACYLLGVPLTLLLGK
                     RIKAGGEDPAR"
     gene            complement(7711..9126)
                     /gene="cysS"
                     /locus_tag="PK442_00060"
     CDS             complement(7711..9126)
                     /gene="cysS"
                     /locus_tag="PK442_00060"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOO86072.1"
                     /translation="MSLVLYNDLTRTKEPFVPLKEGHVGFYSCGPTVYDFFHIGNARP
                     FIVFDVLRRYLEYSGYKVTFVQNFTDIEDKMINRANQEGITVKQLADRFIEEYFKDAD
                     ALGIRRATHNPKATEHIPEIIALIEKLVEKGHAYAADGDVFFDVGSFPSYGVLAKQSL
                     EELQSGARVEINERKRHPLDFSLWKAKKEGEPSWPSPWGEGRPGWHIECSAMSMKYLG
                     ETLDIHSGGTDLTFPHHENEVAQAEAATGKPFVRYWIHNGYLLIDKEKMSKSLGNFLT
                     ARAALQKYPAKAIRLFMLSAHYRSPINFSEESLNQSLGAVERLENCWSDLEHARKNRK
                     TTSDGSRNEFESLFKEFEDRFITAMDDDFNTAAALGVLFEAVKTVNTYLKETQTLEES
                     FLELAAEFFRKIDSVLGVLGLDAAAGEEDAEEIEALIAERNAARKNKDFKRSDAIRDD
                     LAARGILLEDTPDGTKWKKKA"
     gene            9241..10731
                     /locus_tag="PK442_00065"
     CDS             9241..10731
                     /locus_tag="PK442_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOO86073.1"
                     /translation="MAQEHIKKLLKNLPDKPGVYLMRDAEGEVLYVGKAKSLKKRVAS
                     YFRHESFASPRLRKLVASIEDISVIRTETEVEALILEAKLIKKYAPFFNVELKMGDRY
                     PYIKITSEPFPRIVVTRQRGKDDGLYFGPYVRVGDVRSLLRLIERYFPLRICAREIDP
                     ADAAKGKNRPCLHHALGRCMGVCAGLVTPAEYRERVDDVIFLLQGQSTEVVERLRKRM
                     DKAATSLQFEEAARLRDTIRAIWRITRQRVSSPLREDFDGDMWQCLNRLQELLKLPVL
                     PWRIDGFDISHMSGRETYGVVVVFEQGVPNRSLYRRFKIRTVEGVDDFRSMEETLERR
                     YGKSLEGKEPLPQLILIDGGPVQLEFARKALGKLGLEQIPSISLAKEEELIYHSTERE
                     PLRLDRGDEALRLLQRVRDESHRFAIESHRSSRSARLRRSILEDIPGIGKHRAAQLLG
                     RFGSARAIANMSAEELAAAPGIGGATAARILNFLKEQDSDPLQEED"
     gene            10775..>11561
                     /gene="ribD"
                     /locus_tag="PK442_00070"
     CDS             10775..>11561
                     /gene="ribD"
                     /locus_tag="PK442_00070"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOO86074.1"
                     /translation="MERTSPNPLVGCVVERDGVVLAEGWHACCGEAHAERAALEGLDS
                     AAGATIYVNLEPCSHFGRTPPCAPLIVEKGIRRAVVGMIDPDERVSGRGLALLRDAGL
                     DVELGVLEPECRRLNRGFLRKVRLGRPWVTLKGAVGLDGSMALPSGESKWITSEGARA
                     FAHLLRAEHDAVLVGAGTVRADDPELTVRNAWGRSPLRVLLDSTLSVGPGAKILGPSC
                     IVFSSESVSPEWVAAVEASGASVVRVPRKEAGLSLETVLSELVR"
BASE COUNT         2286 a         2969 c         3683 g         2623 t
ORIGIN      
        1 atcgggcaga gagcctccac atgtccgctt ggacgcgacg tcaagctcaa cggctacccg
       61 atccgcgtga gcgaacttct ggcggcgctt cctgcgcccg gctacgtgac ccgcgtgacg
      121 gtggcgcagc caaagtacat catcaaggcg aagcaggctg tgaaacaggc cttccagaac
      181 cagttggacg ggaaggggtt ctccctcgtc gagatactct cctcctgccc cacgaactgg
      241 ggaatgcgtc ctgtggaagc cttcgactgg cttgtcaaca cgatgatgcc ctactatccc
      301 cttggtacgt tcaaggattt cgactaggga gggaagagca tgtcattttt caagacgctt
      361 ctcgcggccg gattcggcgg tcagggagtc atggttttgg gacagctcgt ggcgtatacg
      421 gggatcgaag agggcagaca tgtaacgtgg attccctcgt acggtccgga gatgcgcggc
      481 ggaacggcga attgcggcgt ggtgctttcg gacgacgaga tcggttcgcc cgttgtcgac
      541 aaggcggacg tccttgttgt gatgaaccag ccgtcgcttt ccaagttcat cgacaggctg
      601 aagccgggcg gcgtcctggt gtacaacagc aatctcgtcg aatacgacaa ctcacgtaag
      661 gatattcgcg tcgtcgccct tcccgcgacc accctcgccg aggagctggg cagcgacaag
      721 atcacgaacg tcgttattct gggcgcggtc gtggagtcga gcggtattgt cgccgacgcc
      781 gtgtgcgtgg agacgatcaa ggagaagctg ggtaaaaaga agccgcagtt cctcccgatg
      841 aacctcggcg cgtacgaaaa gggaaaagag gttgccaggg aatgtctgaa ggcgtgaggg
      901 agaagaccct tgcgcagaag accgtctatt ccggcagaat actgaatctc cgtgtggacg
      961 atgttgagct tcccgggggg aagcgggcga tccgcgaggt cgtggagcac aagcctgccg
     1021 tcggtatcct tccagtcatg gaagacggga ccgttctttt gatacggcag taccgttacg
     1081 ctgtcgggga ggatatgctc gaaattcccg caggaattgt cgagggaaac gagcctttcg
     1141 acgatgccgc tcgaagagag cttcaggaag aagtgggcta ctttccggag catttgcggg
     1201 agatcggacg tatctacaac tctccggggt tcagtacgga gctgatggcg ctgtttgtcg
     1261 ctaccgggct gcgtccgtcg aagctccagg aggacgacga cgagttcatc acggttgttc
     1321 ctgttgccaa ggaggagatc ccggccttga ttacgggggg caaactgaag gatgccaaga
     1381 cgtttgccgc gctctcgtgg ttgatggccg ccggtgaggt tcactgagtt tcttgtgaat
     1441 gatccgccaa ctattttgtt gcactctatt acagacgcct gaggaaccgg tttttccggt
     1501 tccttttttt atgctacaat gtaactatta tgacattata tgactttgaa tccgcccatg
     1561 agtcttttct tgcgtggttg ttgctggagc gggggttgag ccggaataca gcctccgcgt
     1621 actctcgtga tcttcacaag tggtttgcgt attgcacgac gaacggaatt cttccattcc
     1681 ctcccgacga ggacggaatt gctcgctttc agcgttttct tgtcgagagc gaggggagca
     1741 aagcttcgcg tcagcggaca atggcgggct tgcgtacgtg gatgcgcttt cttcaggtcg
     1801 aggaggtcct tgaggcggat gtcgcacttc ccccgttgcc gcagagagaa cagcgtcttc
     1861 cgaaaattct cagcgaaggg gaggtggaca gactgctcaa cgtctgtgga ggcgatgatc
     1921 ctctttcgca aagagaccgt gcgctttttg aagtggcgta tggatgcgga ctgcgggcga
     1981 gcgagatctg ttctctccgg gtcggggatg tggattttgc cgcgaagacg ctcagaacac
     2041 ttgggaagag tgagaaggag cgtgctgtgc cgtttcttgg agaaccggcc cacagggtgc
     2101 ggaggtatct tgaagaggcc cggccgcgtc tggcgaagga ggagaccggt attctcttcc
     2161 tttcgcggaa cggtatgatg ctgaacaggg aggatatttg gcgaatcata cagaagaggg
     2221 gagctgaagc gggtatttca aaacaacgtc ttcacccgca tatcctccgt cacagcttcg
     2281 caacgcatct tctccggcgc ggtatggacc agaagacgct ccaggaactt cttgggcatt
     2341 cctccatcgc aacgactgac aagtacctgc actttgacct tgaacttcgg gacgtgtatg
     2401 atgcttctca tccacgtgct taaaacgaaa aaattaagga agaggagttg agagaatgat
     2461 cgaaaaagtc aaggccgcaa gcgctttcct taaagaacgt atttcttcgt ctccggaatc
     2521 tgcggtgatt ttgggttcag ggttgggctc tttcgcggat tcccttgaag ggcgtaccgt
     2581 gataccctat gaagagatac cgcactggcc tctttcaacc gctcccggac acggaggaag
     2641 acttgtccac ggaaggctgg gagatcgtga gctcctcgtc atgcaggggc gcgttcacta
     2701 ttacgaagga tacccgatgg aagaggtagt gttcccggtg cgcgtgctcg ctcagttggg
     2761 cgtgaagaac ctggtgatca ccaacgcttc gggcggagtc gacgtctcgc tctcgccggg
     2821 ggatattgtt cttctggagg atcatgtcaa tttcatggga gcgaatccgc tgcgcggtcc
     2881 cgatactccg gggtggaacg accgtttccc ggatatgacg cgatgctacg acgaaaaatt
     2941 catagccgct ttcgaagcgg caggagcggc tgagggtgcg ccggtgcgca ggggagtcta
     3001 tttcgctttt tcaggaccgt cgttcgagac tccggccgaa atacggatgg cgcgcgcgtt
     3061 gggggcgacc gtggtgggaa tgtccaccgt accggaggct attactgcaa acgcaatggg
     3121 gctgcgtgtc tgcgcgcttt cgtgcgtggc caacgctgct gccggtttga cgatgaacag
     3181 gctgagcggg caggaggtgc tcgacgagat ggcgaagaca tccgaaaagt tgattcgcct
     3241 cttgaatcgt tttttcaagg agatgagatg aaatgttcga tatgatttcg ttcatcgagc
     3301 ggaaacgaga cggcggagcc cacacggcgg aagagattca agagttcgtt cgaggatttg
     3361 cctccgggac cgtccccgac tatcaggcaa gcgcgtggct gatggcggtg tttcacaacg
     3421 gactaaaagg ggaggagctc aaggagttta ccctcgcgct ggctcattcg ggaaagatcc
     3481 tctccttcgg agaccttcgc attgtcgaca aacacagcac gggaggtgtg ggcgacaagg
     3541 cgacgcttct tcttgttcct ctggcggcgt cctgcggtgt gaaggtggcg aagctttcag
     3601 ggccggggct gagttttaca ggcggcacag tcgataaact ggaggccatc cccggttttc
     3661 aaatgcacct ctcttcggag cgttttatcg aacaggtgcg ccggatcggt tgcgctattt
     3721 ccggtcattc cgacgatctt gctccggcgg aggggaagtt ctacgccctg cgtgatgtga
     3781 cgggaacggt gcctacgctt cctcttcttt gcagcagcat cgtgagcaag aagatagccg
     3841 gaggcgctca cgccttcgtc tttgatgtga agtgcggggc gggcgctttt atggaggatt
     3901 atgaaaacgc cgagaagctc gcccgtgcgc ttgtctctct ctccaaatcc ctcggaaaag
     3961 agagcatggc gctgattacc gacatggacc agcctttggg agagtggata ggaaacagca
     4021 cggaggtgct tgaagccgtt tccgttcttc agggcaaggg accgaaggac gtggaagagc
     4081 tcgttatcaa ccttgccgga gcgatggttt ccatagattc ggacctctct ttcgaagagg
     4141 ggtgcgcgct cgcacgggag aagctggagg atggatcggg ccttgaagca ttcgcggcga
     4201 tggtccgcgc gcagggaggg gatgaccggg tatgccgcac tcccgagaaa atccttccgc
     4261 ttgccggcaa aaaacataca atcactgcat ctgaagactc cacggtgcgc gccctgaacg
     4321 cccgaaagat cggggaaggc gtcaagcgcc tcggtggagg gcgcatgtcg ctgggagaac
     4381 ctatcgacct ttcggtggga gtgcggattc tgaagaagaa cggcgcctcc gccgggcggg
     4441 gggaagcgct gcttgagatt ttgtactccg acgaggcgaa actggcagac gccatgccgt
     4501 tttttgaatc cgcctatacg ttcggcaaag cgaacgaaca tagcgaagat acagcgcaaa
     4561 ggagacttat tcttgggaag atcgggtaga tttacgtttt tccgtttcgg cagcattctg
     4621 tttctgttgt tctgcctcgt ttgtatcccg ctgtccgctt cggccgaggt gactgttcag
     4681 tacccctcct cggtgggcgt cgggaagcct tttttagtgc gcgtcacttc ctccgtcccg
     4741 ttgacgggag tggaggccgt ttggcaggga cgtacggtgc aactcggagt ttccgtgtgg
     4801 aacgaacggt acatttcgct cgcgcttctc ggaacgcagg tcggcaaagt gaagaccgga
     4861 gatcatcgtc tgacgctccg tctgctctca ggggggaagc gttctgaaca tcctttctcg
     4921 atcaaggtaa cgccggttca gtacaaggag gaccgcctga cgctgcctga gaagatggtg
     4981 actccgcctt cttcggaact caagcggata gaagccgaaa gaaagcgtac gggcgcggcg
     5041 cttgccacaa tgacggcagg taggcaatgg ggtctgccga ttatcaggcc ggtatccggt
     5101 attgtaacaa gcccgtacgg aagacggcgc atactgaacg gaaaaccgag gagtccgcat
     5161 gcgggggttg acttcagggc ggcagtcggt actccggttc tcgcggcgct tcccggaaag
     5221 gtcgtgctca ccggagatca ttactacgcc gggaagagta tctatatcga ttcgggcggc
     5281 ggggttatat ctcactactt tcatatgcac acgatcgacg taaaaaaagg agatcccgtc
     5341 aagccggggc aggtgatcgg gaagagcggc gtaacgggca gggcaaccgg gccgcatctg
     5401 catttcgggc ttgcgttggc gggccagatg gtgaatcctg aacccctttt ttcggcaacg
     5461 tcttccggga tgctcgaaaa aacggcgctc cggaaagtaa cccctcgctg gaagtgatgc
     5521 agggggatga ggtcggattt ctgtatgctt tcgccgggag gagggaagcg gatgagaggt
     5581 tggaagagag ccttcggcag ctgcgcgctt ttgctttttt tcgcgtcggc tgggtgggcg
     5641 agttctctta tagcggacta tgaggcgcgt tttacgaagt acttcacgga caggatccag
     5701 gcgcgatcag tattgtccgt tattaaggcc tcagaggatg agcttcggcg gggcaagatt
     5761 ccgacgttga acctgtgcat cgaggatgcc gtcgtcgagg gcgttcgcta cgacaggctg
     5821 ctgctcgtga tgcatgatgt tctctttact tcggcggcac gcggcattgt tgtgcattcg
     5881 cacggtgaga gccacctttc tggcggcata tccaaggaga cctttctgaa aacgctctcc
     5941 gagggaatgc cccactttgc cgtatcggaa ctggcgcttc gcaacgggag cgtcatgatc
     6001 aaaggcgtgt atgaacggcg gcttacgttc aaggtccggg cgttgatgcg cttcacagga
     6061 tcttatgtga tcgacggaga cggcgtagca agggtgcgct tcgacgacgc ctcgaacgac
     6121 aatccggcaa tcagcacgct cgatgtggca aaggcgatgg cgaatgcagc gtccgtgctc
     6181 tccttccggg attttttcgg agctcccctc gtcagggagg ttttggtgga tcacgatatg
     6241 gtgtggtttt cagcaaggaa tcaatagaaa gattgttgcg ctgctccggg atgctggact
     6301 ttttcaccgg aagccagtac aattcttgac ggcgaatgga ggcgaatgtg tcccggtggg
     6361 cgccccggac ttcaaatccg gtgggaggcg gtataggccg tctccggtgg gttcgacccc
     6421 cacacgcctc cgccagcagt gtaaaccaaa gagacgcttc ccttcaaggg gaggcgtctt
     6481 ttgcatggta caataatccg aaaacacacc atttcggagg tgatttcgtg attccatggg
     6541 agaaaagagc ggaagagatg gtggaaaaac agatccgcgc gaggggcatt ctttcggaga
     6601 gagttcttga ggcgatgcgc tccgtcccgc gtcatctttt ccttccggaa caccttcgtg
     6661 aaagcgcgtg gattgacagc cctctcccga taggcgaaga acagacgatg tcgcagccgt
     6721 acatggcggc aaaaatgacg gagcttcttg caccggaaga aggagattcg gttcttgaga
     6781 taggaacggg gtccgggtat cagtccgccg tcctttgctc gatgggcgcg aaggtcacaa
     6841 gcattgaacg aatcgccccg ctcgctgaaa gggcgagaga gacgctttct tccctggggt
     6901 acgatgtttc cgtgctgtgg tcggatggac gggagaaggg ggagcttgca ggcgagttcc
     6961 agtgtgtcat tgttacagcc gccgcgcctg cattggaggc gtggtggtcc gaggcgctgg
     7021 cgccgggagg aaggctggtt gcgccggtaa cggttctttc cggaagcgaa cggctgcttc
     7081 tccgtgaaaa gaaggcgccc ggcgaatatg tggatacgtg gcacgattac tgccgttttg
     7141 tacctcttct gcaagggagg acgggcggat attgctgaag aggtgatagg gtgaaagtca
     7201 gtttttttaa aggagcgtcg ccgaaggagt tgttctcggt tcgttctctg gcgctttcgg
     7261 ggcttgtcgc agctctatac gcggtgacga cgattttgtt cgccccgatt tcctacggat
     7321 atgttcaagt gagaatctcc gaagcgctta ctgtgcttcc gtttcttttc ccgcaggctg
     7381 ttttcggcct tttcgtgggg tgcctttttg cgaatctctt tggcggtttc ggcatacttg
     7441 atgtggttct ggggagcgcc gcgacgctgt tggcggggat cgtctcttct gcgatgccga
     7501 ccgtctattt agccgcgctc ccgcctgtgc ttgtgaacat ggttatcgtg ggggggtatc
     7561 tttcgtttat tttggacctc ccctttcccc tctgcgcgat gtatgtcggg atcgggcagt
     7621 ttattgcctg ctatctcctc ggtgttccgc tgacgttgct gcttgggaag cgcataaaag
     7681 cgggcgggga ggaccccgcc cgctgagtcg tcacgctttt ttcttccact tcgtgccgtc
     7741 gggggtatcc tccaaaagga tgcctcttgc ggccagatcg tcacggattg cgtccgatct
     7801 tttgaagtct ttgttctttc gtgcggcgtt gcgttccgcg ataagcgcct cgatctcctc
     7861 ggcgtcttcc tcgccggctg ctgcgtcgag cccgagaacg cccagaacgg agtcgatctt
     7921 ccggaagaat tccgcggcca gttcaaggaa tgattcttcg agcgtctgtg tttctttcag
     7981 ataggtattt accgtcttta cggcctcgaa gaggacgccg agggctgccg cggtgttgaa
     8041 gtcgtcgtcc atagccgtta taaaacggtc ctcgaattct ttgaagagtg attcaaactc
     8101 gttccgggat ccgtcgctcg ttgtttttct gtttttccgc gcgtgctcaa ggtcggacca
     8161 gcagttctcc agacgttcaa ccgcgccgag cgactggttc aggctctcct ccgaaaagtt
     8221 gatgggcgac ctgtagtgag cgctcagcat gaagaggcgg attgccttcg cggggtactt
     8281 ctgaagcgcg gctctggcag taagaaagtt gccgagcgac ttcgacatct tctccttgtc
     8341 gatcaggaga tagccgttat ggatccagta gcgcacgaaa ggtttgcccg tggcggcctc
     8401 cgcctgggcc acttcgttct cgtggtgcgg gaaagtaagg tcggttcccc cggagtgaat
     8461 atcgagagtt tctccaaggt atttcatgga catggcgctg cattcgatgt gccagccggg
     8521 gcgtccctcg ccccacgggc tgggccagga aggttcgcct tcttttttcg ctttccagag
     8581 ggagaagtcg agaggatgcc gtttgcgttc gttgatctcg acccgcgcgc cggactggag
     8641 ctcctcaagg ctctgttttg cgaggactcc gtaggaggga aaactgccca cgtcaaagaa
     8701 gacgtcgccg tcggcagcgt atgcatgtcc tttttcgacc agcttttcga tgagcgcgat
     8761 tatttcgggt atgtgttccg tcgctttcgg attgtgcgtc gcgcggcgaa tgcccagggc
     8821 gtccgcatcc ttgaaatact cctcgataaa acggtccgca agctgcttga cagtgatgcc
     8881 ctcctggttc gcccggttga tcatcttgtc ttcgatatcg gtgaagttct ggacgaaggt
     8941 gactttgtag ccgctgtact caaggtagcg gcggaggacg tcgaagacga tgaacgggcg
     9001 cgcattgcct atatggaaga agtcgtagac cgtaggcccg caactgtaga agcccacatg
     9061 cccctccttc agaggaacga acggttcttt tgtgcgtgtc agatcgttat acagtaccag
     9121 actcattttt cagcacctcc aatgcgttct aatcacgtat tatataatac aattcattag
     9181 gatgcagaac atttttcttc actcggcgaa cagagccgca tgtgaaattc ggaggaagac
     9241 gtggcgcagg agcacataaa gaagctgttg aaaaatctgc cggataaacc cggagtctat
     9301 ctgatgcggg acgccgaggg agaggtgctc tacgtgggga aggcgaagtc gctgaaaaag
     9361 cgggtggcgt cgtacttccg gcatgagtcg ttcgcctcgc cccgcttgag gaagctggtg
     9421 gcttccatcg aggacatctc ggtgattcgg accgagaccg aggtcgaggc gctgatcctt
     9481 gaggcgaagc tgatcaagaa gtacgccccc ttcttcaacg tcgagctgaa aatgggggac
     9541 aggtacccgt acatcaaaat cacctccgag cccttccctc gaatcgtggt gacgcgccag
     9601 agaggtaagg acgacgggct ctacttcggt ccctacgtgc gggtcggcga tgtccgttcg
     9661 cttctccgcc tgatcgaacg gtactttccg ctgcggatat gcgcccggga gatcgatccg
     9721 gccgatgcgg cgaagggaaa gaaccgtccc tgtcttcatc atgcgctggg gcgctgcatg
     9781 ggagtatgcg ccgggctggt gactccggcc gagtacaggg agcgggtcga cgacgtcatt
     9841 tttctgctgc aggggcagtc caccgaggtt gtggaacgtc tccggaagag gatggacaag
     9901 gcggcgacga gtctgcaatt tgaagaagct gcgcggctgc gcgataccat ccgcgcgatt
     9961 tggcgcatca cccgccagag agtctcctcg ccgctacgcg aggacttcga cggggatatg
    10021 tggcagtgtc tgaaccggtt gcaggagctg ctgaagcttc ctgtgcttcc gtggaggatc
    10081 gacggcttcg atatctccca catgtcgggg agagagacgt acggcgtggt ggtggtgttc
    10141 gaacagggag tccctaaccg ttcgctgtac cgccgtttca aaatcaggac cgtcgaggga
    10201 gtggacgact tccgctcgat ggaggaaacg ctcgaacgcc gttacggtaa gtcgcttgaa
    10261 ggcaaggaac cgctgccgca gctcatcctg atcgacggag gccccgtgca gcttgaattt
    10321 gcgcgaaagg ctctcggaaa gctcggattg gaacaaattc cctcgatatc gctggcaaaa
    10381 gaggaggaac tgatatacca tagcacggag cgggaacctc tgcggctgga taggggggac
    10441 gaagcgctcc gccttttgca gagagtccgc gacgagtcgc accgttttgc catcgagagc
    10501 catcgttcgt cgcgttcggc gcggttgcgg cggagcattc tcgaggatat tcccggcatt
    10561 ggcaagcacc gggcggcgca actgcttggg cgtttcggaa gcgcgcgtgc tattgcaaac
    10621 atgtcggcgg aagagctggc ggcggcgccg ggaatcggcg gtgcgaccgc ggccaggatt
    10681 ctgaactttt taaaggagca agacagtgac cctctccagg aagaagattg acgaatattt
    10741 catgcgaagg gctttgagcc tcgctctcag gggtatggag cgtacatcgc ccaacccttt
    10801 ggtggggtgc gtcgtggaac gggacggcgt cgtccttgcc gaggggtggc acgcatgctg
    10861 cggcgaggcg catgcggagc gcgcggcgct cgaaggcctc gattcggctg cgggggcgac
    10921 gatctacgtg aacctggagc cgtgttctca tttcggcaga acccctccgt gcgccccgct
    10981 gattgtcgag aagggtatca ggagggctgt ggtcgggatg atcgatcccg atgagcgtgt
    11041 cagcggccga ggcttggcac tgctccgtga tgctggactc gacgtcgaat taggggtttt
    11101 ggagccggag tgccgccggc tgaatcgcgg ctttcttcgg aaggtccgac tgggaagacc
    11161 gtgggtaacg ctgaaggggg cggtcgggct cgacggttcg atggcccttc cttccgggga
    11221 gagcaagtgg attacttcgg agggggcgcg cgcttttgct catcttctga gggcggaaca
    11281 cgacgctgtt ctggtgggcg ccggaaccgt ccgtgcggac gaccccgagt tgaccgtgcg
    11341 caacgcgtgg gggcgatcgc ctctgagagt gctgctcgat tccacgctct ctgttggccc
    11401 gggagcgaag atccttggcc cgtcgtgcat cgtcttctcg tcggagtcgg tttctccgga
    11461 atgggttgcc gcagtcgagg cttcaggagc ctctgtcgtg cgagttccgc gaaaagaagc
    11521 cggtctctct ttggaaacgg ttctctccga gctggtgcgc a
//
LOCUS       DAOSWW010000002        11030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_3759_length_11030_cov_4.037722, whole genome shotgun sequence.
ACCESSION   DAOSWW010000002 DAOSWW010000000
VERSION     DAOSWW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3759_length_11030_cov_4.037722"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..723)
                     /locus_tag="PK442_00075"
     CDS             complement(94..723)
                     /locus_tag="PK442_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOO86075.1"
                     /translation="MKRVIGRLALLIVLLLAFSAVSEGALDRATVDRAWTRIAAHAGI
                     PWQQVIYEEQQEPNAWVKFSPGNHTVHVTTGLMAILTKEDEIAGILAHEAGHIQLGHY
                     SSSVSRNLLWGLLFNSLKGDAAREIVGGVGMVLAESGFSREQEVEADDYGIRVSSAAG
                     YSPWGLVRAMEKMKAAGFKTSPSGFNSHPPTERRLLRLRNNAAAIAPRN"
     gene            877..2007
                     /locus_tag="PK442_00080"
     CDS             877..2007
                     /locus_tag="PK442_00080"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOO86076.1"
                     /translation="MLHCFQCGKTFDEASLLWRCPCGGVLDWTVPEPFKRSSIRTEET
                     GMWRYAASLPLDSPDSRVSMGEGGTPLIPGDWNGLSVFWKLDFLCPTGSYKDRGMACL
                     VNRLRELGVRELIEDSSGNAGSSMAAYCALAGIGCKIYVPGYTSEGKCVQILSYGGEL
                     VRIPGTREDTSRAAEKAASETYYASHNWSPYFAHGVKTFAFEVWEQLGFQLPDAVLLP
                     AGQGSLVLGWAQAFEELRASGEIDRLPRIYALQPENCAPLALAFEKRADAPEPFPKKE
                     TIAEGISSAEPVRGVQVLSAVRRSGGAVRALSERAIWDGFNKLARKGLYVEPTSAIVG
                     AALDELRNAGELREGERLVALLSGSGLKATDKILSLIGNPPA"
     gene            2061..3299
                     /locus_tag="PK442_00085"
     CDS             2061..3299
                     /locus_tag="PK442_00085"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOO86077.1"
                     /translation="MGRVTVNNLQEGMVLDEDLRAPNGRFILGKGVTLKEKYIRMFKI
                     WGITGADVEGVEDGEVVRRALLEEDAIRRSRSYVDTYFPPEDAEDVCASAVRSFCVRR
                     FAEEFASGTPPSPLPEVKSLPRPPEGHFGRSFSAYALAKDEVRLFSFPDIYFKIQEVI
                     NSPISSATSIAKVVGNDPNLAARLLKLVNSSFYGFPNPIQSIPRAIAIIGSNELTSLA
                     LAVSTMKVFKNVPKEYVDMKSFWRHSIACGVFSRLLAYNKRLRSEERFFLAGLLHDLG
                     RILLYTKAPNEMTYALELSLFEHIPLWRAEKRVFGFDHTAVGKTLMEEWRIPRGISLL
                     CDSHHNPTSEEAPEEAVFVHLADYLANSLRIGTSGSLYFSGVDRALWELLELDEGDVE
                     AVLVQGERQIREIMNIFLMT"
     gene            3307..5523
                     /locus_tag="PK442_00090"
     CDS             3307..5523
                     /locus_tag="PK442_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86078.1"
                     /translation="MKPSDESIDQLRERVRVLTGEREATKRILEAAVDSLAFPVVVDE
                     TFSSDVLLAQAAQKLRSFIKLDALVFYLFTPDGLDFFPAFCDPPERLSFFEEEMIPLV
                     DDGTFAWAVDRNKPVIITARPGKTSAGLEVKERQLLLHSIMSSNRSIGMFMSLLGEDE
                     TDILDVSFAFFTVLLGSMASILHNAELYTMIQGLNNELQGKIKRLEESERSLAEAMKA
                     KEVFLANVSHEVRTPLNGIVGMTTLLEETPLDARQRELLGVLKDESGALLRLINDLLD
                     FSKMEAGKLVFEDAPFDLYELWNSVRDSFLPRAVAKNLSFSMTLDESAPRVVSGDSFR
                     IRQVLGNLVGNAVKFTGAGSVEVNGNCLARREESFFFEVTVRDTGIGISTKKKDELFQ
                     PFVQGDVSMTRKYGGTGLGLAISKRIVDGMGGRITLESREGEGSLFSFTIPMREVRVP
                     EGPFPQHFREKEAREDTYASASILVVEDNPTNRTVAVALLNLLGLQNIETAENGEEAI
                     EKLSATQYDLVFMDIQMPLLDGLQALAKIRDPSSKVVDHETPVVAMTANALPGERERY
                     IEAGMSDYISKPILAAELRRIVRRFLSEDKRDSRHSDSGRFVLSEANMKSPDSSEIPA
                     FRPAVLLDRMGGDRDLCRQTITLFIEDSAKIVKIISSSVASGNAEESRRMIHALRGAS
                     ANVEAFALALLAADAEQAAERGDLEEAGRALPKIERAREKFIHAFREFDTPISDTK"
     gene            5569..6498
                     /locus_tag="PK442_00095"
     CDS             5569..6498
                     /locus_tag="PK442_00095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO86079.1"
                     /translation="MKILVAEDDATSLFMLQSLLMKWGYDVIAASDGQEALRVLTGEN
                     PPLLAILDWMLPGESGPEICRKLASRERLREGNAAREEMMKPYQYVIILTVKGEKENV
                     VAGLEAGADDYITKPFDAHELKMRLMVGRRILDLQEKLRNAALFDALTGLLNRRAVIE
                     RLEQELARASREATPLSVALLDIDHFKDVNDTYGHASGDAVLVESAQRIRSSVRPYDI
                     VGRYGGEEFLMVFPGAEGSALDGICERIRNAFQGAPFYSAENDGDRSTMDITVSIGIS
                     EYKKDNDNVDFILAEADRAMYRAKRSGRNRVSR"
     gene            6972..8684
                     /locus_tag="PK442_00100"
     CDS             6972..8684
                     /locus_tag="PK442_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOO86080.1"
                     /translation="MAYALFRLADEKDHDGRKKYLQRMYALAENNAQRTTALTNLLKL
                     PGNKEAYALNLTNISPRNSAALKVLESLPKPWNPDVNMAVGYAAYLRGQYDKAIPLFR
                     AIPMDSRHGRKSRYYRAFSLYNQKKYTAALEAWGYLAKTGTSYAESSIRRISILAGRA
                     ERESALSVLREVAASRDGEIQARAYYSLSTHLSGKEKREAEDKVIALAPNSLFTTQIL
                     WRRGWGKWASGDVRGAVADWERSLVPEMKTSWRPRVLYWVSKGYGRLNQPSKRDTFVK
                     TLLKDHPLSIYAFLSGGEKLDIQAGDPPSLASRQPSELEKWGFVSHARRVLLAKGDAP
                     SIYRAALLAEWMGDQQAAYSAAGRVSEQIRKGPVFYRRALSFLFPRPFFSEVTKASDR
                     FKVEDNIVWAIMRQESAFNPNATSWVGARGLMQLMPGTARSEAKALEMKKYSVYNPEQ
                     NIILGSAHIARLLRSFGDVEVALAAYNAGGGNARKWLDGRKDVPLDEWIESVRFEETN
                     DYVQKVMANLHIYRTLYAPSRLSASDAAAPGDVPDEDAEPGSAGSEPEESDDAPVPAD
                     DGGK"
     gene            complement(8719..10839)
                     /locus_tag="PK442_00105"
     CDS             complement(8719..10839)
                     /locus_tag="PK442_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HOO86081.1"
                     /translation="MLKVKEAMPKDVGHSIARISPEDMAALGLSEGQIVEIEGKRRTP
                     VRILSCDSDLVASGGAKGVVQIDGITRDNAQASIDDKVSVHTTTHHFAGSVTLRPLTS
                     TALLEKERDAAYIRSLLEGLPVLTDDRVRTNLFGARICDFRVVDTTPAGTVIISKSTY
                     VNVEKQAEGEKKAKISYEDIGGLGPQIQRIREMIELPLRFPQVFDRLGVQPPKGVLLY
                     GPPGTGKTVIARAVASETDVYFTHISGPEVIGKFYGESEERLRNVFEDAQAHAPSIIF
                     IDEIDAIAPKREEMGGEKQVERRVVAQLLALMDGLESRGQIVVIGATNIPNTLDPALR
                     RPGRFDREISIPIPDKKGRYEILTIHTRGMPLAADVDLRRLADMTHGYVGADIEALAK
                     EAAMASLRELLPEIDMEMEEIPYDRLLAMDVRMDHFLDAFREVDPSAIREVFVEIPDV
                     SWRDIGGLEDIKEELHDAVQWPLEHADIFEKFAISPPKGIMLHGASGTGKTLLAKALA
                     HESGVNFISVKGPALMSKYVGESERAIREVFRTAKQAAPSILYFDEIESLVPVRGKDS
                     GSGASFTERVISQFLAEMSGIEELKGVVVLATTNRLDLVDPSLLSSGRFDLLLELPKP
                     DAAAREKIFEIHLRKKPLSAEIRIPTLARLTDGRSGGDIAFVCRKASMMAIRDHIKGG
                     GEAEPLLEWRHFETALKELKNVLS"
BASE COUNT         2458 a         2870 c         3143 g         2559 t
ORIGIN      
        1 gtcaaaaggt gtgaaggagc tccggtggta aaacaacaaa aaagaactac acccggagga
       61 atcatcctct cctccgggtg tttaagaaat gtgtcagttc cggggggcta ttgccgccgc
      121 gttgttcctc agacggagca gtctgcgctc cgtaggaggg tgggagttga agccgctcgg
      181 ggaggtcttg aatccagcgg ctttcatctt ttccatggcg cgtaccaacc cccaggggct
      241 gtatccggct gccgacgata cccgaatacc gtagtcgtct gcttcaacct cttgttccct
      301 gctgaatccg gattcggcaa gaaccattcc gaccccgccg acgatctccc tcgcggcatc
      361 ccctttcagg gaattgaaca gaagccccca gagaagattg cggcttacgg agcttgaata
      421 gtggccgagc tggatgtgtc ccgcttcatg agcaagtatg cccgctattt cgtcttcttt
      481 ggtcagtatc gccatcaggc cggtcgtcac atgaacagtg tgattgccgg gcgaaaattt
      541 aacccaggcg ttcggttctt gctgctcttc ataaatgacc tgctgccatg ggataccggc
      601 atgagcggca atacgcgtcc acgcccggtc taccgtcgct ctgtcgagag cgccctcgga
      661 aacagctgaa aacgccagaa gaagtacgat aagaagagcg aggcgtccga ttaccctctt
      721 cattcgaatt cctccttttc tctttagtgt agttctattt tccaatagga tacatccttt
      781 cttcttttag tgaaaggggg aaacttcgta ggagtcaata ggcagtatcg gacattctgt
      841 atataatgaa atcagaatcg agaggaggtt gattgcatgc tgcactgttt tcaatgcgga
      901 aaaacgttcg acgaagcatc cttattgtgg cgctgtccat gcggaggtgt tctcgactgg
      961 actgtacccg agcccttcaa acgctcgtcg atacgcacgg aagagaccgg gatgtggcgg
     1021 tacgctgctt ctctgcctct tgattctccc gattcgcgtg tttctatggg ggagggaggg
     1081 actcctctta ttcctggaga ctggaacggg ctttccgttt tctggaagct tgattttctc
     1141 tgccctacag gctcatacaa agaccgcgga atggcgtgtc ttgtcaatcg tttacgcgaa
     1201 ctcggcgtgc gtgagctcat agaggactcc tcaggaaatg cgggctcgtc gatggccgca
     1261 tactgcgctc ttgccgggat cgggtgtaaa atctacgttc cagggtacac gtccgaaggc
     1321 aagtgcgttc agatactctc ttacggcggg gaacttgtcc gtatacccgg taccagagag
     1381 gatacttccc gtgcggcgga gaaagcggcg agtgaaacct attatgccag ccataactgg
     1441 agcccctact tcgcccacgg cgtaaaaacc tttgccttcg aagtatggga gcagttggga
     1501 ttccagcttc cggacgcggt tcttcttcct gccggtcagg gaagtcttgt tcttggatgg
     1561 gcgcaagcat tcgaagaact gcgcgcctct ggagagatag accgtcttcc gagaatctac
     1621 gcgcttcagc ccgaaaactg cgcacctctg gcgctcgcct tcgaaaagcg ggccgacgct
     1681 cccgagccgt tcccaaagaa agagaccata gcggaaggta tcagttctgc ggaaccggtt
     1741 cgcggggttc aggttctttc cgctgtgcga agaagcggag gggctgtccg tgccttatct
     1801 gagagggcta tttgggatgg ctttaataag cttgcacgga aggggctgta cgtggagccg
     1861 acaagcgcga tcgtcggagc agcccttgac gagctgcgga acgcgggaga actccgcgaa
     1921 ggagagcgtc ttgtcgcgtt gctttccggt tccggactga aagcgaccga taagattctc
     1981 tcgctcatcg gcaatcctcc ggcctgatac ttcttcttgc tgtggtatag ttatcctatt
     2041 ttgagccggg gagagaaaat atgggccgtg tgactgtgaa taaccttcag gaaggaatgg
     2101 tcctcgacga ggatcttcgc gccccgaacg ggcgtttcat ccttggaaaa ggggtgaccc
     2161 tgaaggaaaa atacatcagg atgttcaaga tttgggggat taccggcgcc gacgtcgaag
     2221 gcgtcgaaga cggtgaagtc gtccgaaggg cgctcttgga agaggacgcg atccggcggt
     2281 cccgttccta tgttgatacg tacttccctc ccgaggacgc cgaggatgta tgcgcttctg
     2341 cggtccgttc tttttgcgtg cgccggttcg ccgaagaatt tgcttccgga acgccgcctt
     2401 ctcctttgcc ggaagtgaaa agccttccac gccctccaga gggacatttt ggacgctctt
     2461 tctctgcata cgcactggca aaagacgaag ttcgtctttt ctcatttccg gacatttatt
     2521 tcaagataca ggaggttatt aactctccga tcagttcggc tacgtccata gcaaaggtgg
     2581 tcggcaacga tccgaacctt gccgcccgtc tgctcaagct tgtgaacagc tctttctacg
     2641 ggttccccaa cccgatccag tcgattccca gagcgattgc gatcatcggt tccaatgaac
     2701 tgacctcgct tgcccttgcg gtttcgacca tgaaggtttt caagaacgtc ccgaaagagt
     2761 acgttgacat gaaatctttt tggagacatt caatagcctg cggagtcttt tcacgccttt
     2821 tagcatacaa caaacggctt cgttcggaag agcgcttttt tctcgccggg ctgctgcacg
     2881 atctcggccg gatactcctt tacacaaagg ctccaaatga gatgacctac gcgctcgaac
     2941 tttcgctttt tgaacacatt cccctctggc gtgccgagaa gcgagtattc ggcttcgatc
     3001 ataccgctgt agggaagacg ctcatggagg agtggagaat tccccggggg atcagtcttc
     3061 tctgtgattc ccatcacaac cctacgtcgg aagaagcccc cgaagaagcg gtattcgtcc
     3121 atcttgcgga ttatctggca aacagtctgc gtattggcac cagcggctct ttatatttct
     3181 cgggagtgga tcgcgcgttg tgggagctcc tggaactgga tgagggcgac gtggaggctg
     3241 tgcttgtcca aggagagcgt cagatacgcg agataatgaa tatcttttta atgacgtaat
     3301 gtaaagatga aaccttcaga cgagagtata gaccagcttc gggagagagt tcgggtactt
     3361 acaggagaaa gagaggcgac aaaacggatt cttgaggccg ccgtcgactc tcttgctttc
     3421 cccgttgttg tcgatgaaac cttctcttcc gacgtcttat tggcccaggc tgcgcagaaa
     3481 ctccgctctt tcatcaagtt agacgctctt gtcttctatc tctttacacc ggatgggctg
     3541 gactttttcc ccgcgttttg cgacccgccc gaaagactct cctttttcga ggaggaaatg
     3601 attcctctcg tggatgacgg aacatttgcc tgggctgtcg accggaacaa accagtcata
     3661 atcaccgctc gtcccgggaa aacgagtgcg ggtcttgaag taaaagaacg ccagcttctg
     3721 ttgcattcaa taatgtcgtc aaaccgcagc atcgggatgt ttatgagtct tttaggagag
     3781 gacgaaacag atattcttga tgtctctttc gccttcttta cagttttgct tgggtctatg
     3841 gcaagtatcc ttcacaatgc cgagctgtat accatgattc aaggtttgaa taatgagttg
     3901 caaggtaaaa tcaaacggct ggaggagtcg gaacgtagtc ttgcagaagc gatgaaggca
     3961 aaagaggtct ttcttgcgaa tgtcagtcat gaagtgcgta cgccgctcaa cggtattgtc
     4021 ggtatgacga cactgctgga ggaaacgcct ttggatgcgc gccagagaga gctccttggt
     4081 gttctgaagg acgaatcagg agctcttctc cggctgatca acgatcttct ggatttttca
     4141 aagatggagg cgggaaaact tgtcttcgaa gacgctccct tcgacctgta cgagctttgg
     4201 aacagcgtcc gcgacagttt ccttccgcgc gccgtcgcaa aaaacctctc gttttcgatg
     4261 acgctcgacg aatcggcgcc ccgagttgta tccggcgact ccttcaggat acgccaggtc
     4321 ctcgggaatc tcgtggggaa tgcagtaaaa tttaccgggg caggatctgt cgaagtcaac
     4381 ggtaactgct tggcacggag ggaagaatcc tttttttttg aagttacagt ccgagatacg
     4441 ggcataggga tttccacgaa aaagaaggac gagcttttcc agccttttgt ccagggtgac
     4501 gtttcaatga cccgaaagta tggcggcacg gggctcggtt tggctatttc gaaacgcatc
     4561 gtcgacggta tgggaggccg gattacgctg gagagccgcg agggggaggg ctccctcttt
     4621 tccttcacta ttcccatgcg cgaggtccga gttcccgaag ggccctttcc gcagcacttt
     4681 cgggaaaagg aggcgcgaga agatacgtat gcgtcggctt ccatactcgt tgtggaggat
     4741 aacccgacaa acaggaccgt ggcggtcgca ctgctcaatt tgctcggtct gcagaatatc
     4801 gaaacggctg aaaacggtga agaagctatt gaaaagctct ctgcaacaca atatgattta
     4861 gtcttcatgg atattcagat gccgttgctc gatggacttc aggcgcttgc caaaattcgg
     4921 gatccatcgt ccaaggtagt ggatcacgag actcctgtgg tagcgatgac ggcaaatgct
     4981 ctccctgggg aaagagagcg gtatattgag gcggggatgt cagactacat atccaaaccg
     5041 attcttgccg cggaactacg aagaatcgtc cggcgctttc tttcagaaga taaacgtgac
     5101 tcccggcatt ctgattctgg gaggttcgta ctctctgaag ccaacatgaa aagccccgat
     5161 tcgtccgaga ttcccgcttt tcgccctgcg gttctgctgg atcggatggg gggagatcgc
     5221 gatctgtgcc ggcagacgat tactcttttc atagaggact cggcgaaaat cgtgaaaata
     5281 atcagctctt cggtagcgtc ggggaatgcg gaggaatcac gccggatgat acacgcgctt
     5341 cggggcgcct cggcaaatgt ggaagctttt gctcttgcgc ttttggctgc cgatgcggaa
     5401 caggcggcgg agcgcggaga tcttgaagaa gccggacggg ctcttccaaa gattgagagg
     5461 gcgcgcgaga aattcattca cgcctttcgc gaattcgata cacccatttc tgatacaaag
     5521 taaatgcgct ccgatatacg tgtgtttaag gaaaaaggag agattcggat gaaaatattg
     5581 gtcgcagagg acgacgcgac atcgctcttc atgcttcaat cgctgctcat gaaatggggg
     5641 tatgacgtta ttgcggcctc ggacgggcag gaagcgctgc gcgtgctgac aggagaaaac
     5701 ccgccgctgc tggcgatact cgactggatg cttcccggtg aaagcggccc cgaaatttgc
     5761 cgaaaactcg cctccagaga gcgcttaagg gaggggaacg ccgcccggga agagatgatg
     5821 aaaccatatc aatacgttat catcctcacc gtgaaaggag agaaggaaaa tgtggttgcc
     5881 ggtttggaag caggagccga cgactacata acaaaaccct ttgacgcaca tgaactgaaa
     5941 atgcggctca tggtagggag gcgcattctt gaccttcaag aaaagcttcg caacgccgct
     6001 cttttcgatg cattaacagg acttctcaac agaagagccg tcattgagcg tcttgaacaa
     6061 gaactggcgc gggcatccag ggaagcaact cctttgagtg ttgcgctttt agacattgat
     6121 cattttaagg atgtcaacga tacttacggg catgcctcgg gagatgcggt gctggtggaa
     6181 agtgcgcagc gaatacgttc ttcagtccgt ccgtacgata ttgttggcag atacggcggg
     6241 gaagaatttc tcatggtttt tcccggcgcc gagggaagcg cgctggatgg tatctgcgaa
     6301 agaatacgga atgctttcca gggagctcca ttttattcgg ccgagaatga cggtgatcgg
     6361 tcaacgatgg atataacagt gagtattggt atttctgaat ataaaaagga taatgataat
     6421 gttgacttta ttctggctga agcagacaga gcaatgtaca gagcgaagag aagcgggaga
     6481 aatagggtaa gccgctgact actgtcggta attctaccgg agcggcagga agctctctca
     6541 cgtagaatgg ccgggatatt cgccgaaagg tgttgagagg agtgttttcg gtgtcccgac
     6601 gattgtttcg tctttctttg ttttttgtgt tctctgcgtt gctttttgtc ttccctttga
     6661 gtgcatacag ccagtcaatg gaagatcttt tccaacgccg ggactggaag ggaattgatt
     6721 cccttcttgt ttcgggaaat gctctcacgg ccgtggatta ctcacttgcc gccaatgctc
     6781 tgtggttcca gaatcggtgg agtgaatcac ttgttactct caaaaagagc gcatcttcat
     6841 ggccatccgg ggtaaaacca tacggggatt ttatgattgc acttgccctg gagagggcag
     6901 gacaaaaaaa tgaagcccgt aaaatggctc ttgcactctt acctaaagct cctgcagatc
     6961 tcgggtacta tgtggcttac gctctttttc gcctggccga tgaaaaggat cacgacggaa
     7021 ggaagaaata cctccagcgg atgtacgctc ttgcggaaaa caacgcacag cgcacgactg
     7081 ctctgacaaa tcttctaaag cttccgggca acaaagaggc gtacgctctg aacctgacga
     7141 acatctcgcc tagaaacagc gccgctttaa aagttttgga gtctcttccc aaaccatgga
     7201 atcccgacgt caatatggcc gtgggatacg cggcgtatct ccgggggcag tacgataagg
     7261 cgattcccct cttccgggca attccaatgg attcaagaca cggcagaaag tcgcgctact
     7321 accgtgcgtt ttcattgtat aaccagaaaa agtacaccgc cgccctggag gcgtggggat
     7381 acctcgcgaa aacagggacg agttatgccg aatcttcgat ccggcggatt tctattcttg
     7441 cagggcgggc ggagagagaa agcgctctca gcgtgctccg ggaagttgct gcgtccagag
     7501 atggcgagat ccaggcccgg gcctattatt ccctctctac ccacctttcc gggaaggaga
     7561 aacgggaggc ggaagacaaa gttatcgcgc tcgctcccaa ctcgctgttc actacgcaga
     7621 tactgtggag aagaggatgg ggcaagtggg cttcaggaga tgtccgcggg gcggttgccg
     7681 attgggagag aagccttgtc cccgagatga aaacgagctg gcggccgagg gtgctctact
     7741 gggtctccaa ggggtacggg cgtttgaatc agccctcgaa gagagatacg ttcgtgaaga
     7801 cgctcctcaa agatcaccct ctctccatat acgcctttct gagcggcggg gagaagctcg
     7861 atatacaggc cggagatcct ccctcgcttg catcccgcca gccttcagag cttgaaaagt
     7921 gggggtttgt ctcccatgcg agacgcgttc tcctggcgaa aggagatgct ccttcgatat
     7981 accgtgcggc gctccttgcg gagtggatgg gagaccagca agccgcttac tccgcagccg
     8041 gcagagtatc ggaacagata cggaaggggc cggtttttta cagaagggcc ctctccttcc
     8101 tctttccccg accgttcttt agcgaagtga ccaaggcatc tgaccgtttc aaagtggagg
     8161 ataacatcgt ctgggcgatc atgcgtcagg agagcgcctt caacccgaac gcgacaagct
     8221 gggtcggcgc gcgcggcctg atgcagctca tgccaggcac ggcgcgaagc gaggcgaaag
     8281 cgctggagat gaaaaagtat agcgtctaca acccggagca gaacatcatc ctgggcagtg
     8341 cgcacattgc gcgtcttctg cgctctttcg gcgacgttga agttgcttta gcggcataca
     8401 acgcaggagg aggaaacgcc cggaaatggc ttgacggtcg gaaggacgtg ccgctcgacg
     8461 agtggattga atccgtccgt ttcgaggaaa ccaacgacta tgtgcaaaag gtgatggcga
     8521 acctgcatat ctatcgcacg ctgtatgcgc cttcacgtct ctctgcctcg gacgcggccg
     8581 cgccgggaga cgttcccgac gaggatgcgg agccggggag cgccggatcg gagccggagg
     8641 agagcgacga cgcccccgtt cctgctgatg acggaggaaa ataaagaagg gcggaccgga
     8701 taagggtccg cccttcttct atgataaaac gttctttagc tctttgagcg cggtttcgaa
     8761 atgccgccat tcgagaagag gttcggcttc gccgccgcct ttgatgtgat cacgtatcgc
     8821 catcatcgac gccttgcggc agacgaaggc gatgtcgcct cctgaacgcc cgtcggtcag
     8881 cctcgccagc gtcggaatac gaatctccgc agacagcggt ttttttcgaa gatgaatctc
     8941 gaagatcttc tccctggcag cagcgtcggg cttcggtagt tcaaggagga ggtcgaatct
     9001 gccgctggag agaagcgaag gatccacaag atccagacgg ttcgttgttg caaggaccac
     9061 cacgcctttc agctcttcta tgccgctcat ctctgcaagg aactgactga taactcgctc
     9121 tgtgaagctc gctccgctgc cgctgtcctt cccccggacc gggacaagag attcaatctc
     9181 gtcgaagtag agtatggaag gagcagcctg cttcgcggta cggaagacct cgcggatggc
     9241 gcgttcgctc tcgccgacgt atttcgacat gagcgccggt cccttcacgg agatgaagtt
     9301 cacgccgctc tcatgggcga gcgcctttgc gagaagcgtt ttgcccgttc ccgaagctcc
     9361 atgaagcatg atacctttgg gaggcgagat ggcgaatttc tcaaagatgt cggcgtgttc
     9421 cagaggccac tggaccgcgt cgtgcagctc ttctttaata tcttccaaac cgccgatatc
     9481 gcgccacgat acatcgggga tctcaacgaa aacctcccgt atcgccgaag gatcgacctc
     9541 cctgaacgcg tcgaggaagt ggtccatccg tacgtccatg gcaagaaggc ggtcgtaggg
     9601 aatctcttcc atctccatgt cgatctcggg gagcagttcg cgaaggctgg ccatcgcggc
     9661 ctccttggcg agcgcctcga tatccgcccc gacgtatccg tgcgtcatgt ctgcaagacg
     9721 gcgaagatcc acgtcggccg ccagcggcat cccgcgcgta tgaatggtga gaatctcata
     9781 ccggcccttc ttgtcgggga taggtatgga gatctctctg tcgaagcggc ccggacgcct
     9841 gagcgccggg tcgagagtgt tcggtatgtt ggtggcgccg atgaccacga tctgaccgcg
     9901 ggactccagc ccgtccatga gcgccaggag ctgggcgaca accctgcgct cgacctgttt
     9961 ttctccgccc atctcctcac gcttcggggc gatggcgtcg atttcgtcga tgaagatgat
    10021 cgagggcgca tgggcctgcg cgtcttcgaa tacgttgcgc agccgttctt cgctttcacc
    10081 gtagaacttg ccgatgacct ccgggcccga aatgtgggtg aaataaacgt ccgtctccga
    10141 ggctacagcc ctcgcgataa cggtctttcc ggtacccgga gggccgtaga gcagtacgcc
    10201 cttcggtggt tgaacgccaa ggcggtcgaa gacctgaggg aagcgcagcg gcagttcgat
    10261 catctcgcgg attctttgga tctgaggtcc aagccccccg atgtcctcgt aggatatctt
    10321 tgccttcttc tcgccctccg cctgtttttc cacgttcacg taggtggact tgctgatgat
    10381 cactgttccg gcaggcgtcg tgtcgacgac gcggaagtca cagatacgcg cgccgaagag
    10441 attggtacgc acccggtcgt cggtaagaac gggaaggcct tcaagaaggg aacggatata
    10501 cgcggcgtcg cgctctttct ccagcagcgc cgtagaggta agcggacgga gcgtaacgct
    10561 tcctgcgaag tggtgcgtcg tcgtatggac ggacaccttg tcgtcgatac tcgcctgcgc
    10621 gttgtccctt gtgataccgt caatctggac cacgcctttc gcgcccccgg aggcgacaag
    10681 gtcggaatcg catgagagaa tccggacggg agtgcggcgt ttcccttcta tttcgactat
    10741 ctggccttcc gaaaggccca gcgcggccat gtcctcgggg gagatgcggg caatactgtg
    10801 tccgacatcc ttaggcatag cttccttgac tttcagcatg tgtaacttcc tctcttcatt
    10861 gaatgttctg cggaaaacat aataacatac cctgcggccg ccgttcatcc ggaccgcagg
    10921 gtatggaagg gcggagaatc cgtgtttacg gaagaatcag cgggcggcta ccggctcgat
    10981 acgccccgga taggcttcaa gagcgacctt gagaatatcc attgccttaa
//
LOCUS       DAOSWW010000003        10233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_4129_length_10233_cov_3.844174, whole genome shotgun sequence.
ACCESSION   DAOSWW010000003 DAOSWW010000000
VERSION     DAOSWW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4129_length_10233_cov_3.844174"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..3704)
                     /locus_tag="PK442_00110"
     CDS             complement(117..3704)
                     /locus_tag="PK442_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86082.1"
                     /translation="MRGKFKKIAGVLFVLCIAAFLAAFFLISGQDAGTSYFKTELEKA
                     VATLAGWTLETDGISGNFLAGYNAANIRILFEGQEVARAEELSVGLSILSFFRGQSGL
                     TKVTVTEGVLSGEGMLHALRRSDFSAGSGITTDFMVPVILFSPVTITTPLGEISLASL
                     RLTPGEKTMTFHGEGHFLDFPVEAGASFTTGEQLSITDGFLRGGNAVVSFSGPLFPET
                     RIEGNIDDLRLDVISELTNLPFTAKGTVDSTLLLERPEGRLLVSGEGEIENGDIWDLL
                     MDGTFSWSADESKAVVAPVDARVFSSPASGVFALYFGEEPTAEIAFALKQLKIEEWTH
                     CFSWLSFAQGTLSTLKIDLSGPFEKLNGPILFEAPKATLEGFDVRALRGDLRMFDGER
                     IAISGNATWSDSPFTISGKSLLDDSAGAETSISLHSGSFDLKSSGKVYAPELSADGKG
                     TMALEISIPSEGAASYKGALAAPRASFLGTAGEKLSVAFSGTEEILSLSALSFSPAGK
                     GLLSGKGSITGLSGKSPSITMEGSGRDLAWNLLRDALGGLETAGLFDASWSVKGPTAS
                     PVVSFGLKGRETPFSRSLPLRNIQLEGRFEKNELSVSKGSGSLFGGTANIEGKARFGS
                     APSLSFKGAFRGLSAGQIAAAAGADAETADGVVAGEFSLSGKPSSPLLSLTASAPSLG
                     VASLPLTAVRAKAEGTLSSLKITDFSARLQESSVDLSGVIGLSKGEKTNLKIAANKLD
                     LHSLAAAFFPGVRLGGTLSAQLNVSGKTGGAYTSTLSGTSPFLSVYGVLLEKAKVSLT
                     PDGEDAFDVALEGMLGESALALEGRAVVSPSGATFSLKNTKKIDLAKTASALSAHAAG
                     IFTGDADFSLTGKLGAVPTFEGSISAQSLGLYKTEARSVSVPFRWSDGMFVISGGKGV
                     YHEGTALFNGKVDPVTMRWEGNLAVKGMDLASASERILEGQGKISGKADLTMRGSGTG
                     GMLGLVFGSGQISAKEGSVSGFESIKSVSKTGEIRFSSLLASFNMDGRSIFLLPGSRL
                     SAPPGDTVYRYFSASGSVGWNDSPLDLKCVGDINIRALNAFIGALQGFLTIDGNPLTD
                     PQFLQRFLTGLVGGMAIRDFRETSFNLKGTWDSPEMLDLKVSRGSAPLTIPRSNGSTG
                     KNETQIKITVEIPTGSGSDTSASTEEQVKKQLLENIMKSIIKPGSTEND"
     gene            complement(4005..4793)
                     /locus_tag="PK442_00115"
     CDS             complement(4005..4793)
                     /locus_tag="PK442_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOO86083.1"
                     /translation="MRRIALFLLLFALFFVLFLFFRAVPFQRDEQPFPPPGVRVFDGD
                     TAELPSGGGVARVRYLLVDTPELHHPSRPAEELGEEARLLNSRLLASGPLRLEYDSER
                     TDRYGRTLAYVFAGGEGSELFLNEILVREGLAQPLIIPPNRRYAGRILKAFEEARAGQ
                     RGLWNRAEGRVFTSAQVWSEAPLLAGSFLTLFMTIEKAELKGRRILLREGRVTVVAYK
                     SPETESLLSLREGDRIRAVGKLLLSYNGCELPVASEIQVSRSTE"
     gene            complement(4790..5548)
                     /locus_tag="PK442_00120"
     CDS             complement(4790..5548)
                     /locus_tag="PK442_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOO86084.1"
                     /translation="MRLYDSILQEVLKAPDGAVDNLIIGWKGSFLSLSDGRWGVGSTP
                     PEGGEPMSPREEHTRNILMSGAHSLARLLVSPYPQEFSAASASLAALAPAPEGGEPLE
                     FLLQLPGRDRVALLAPDPWVTDFLRDWNWKISIFDDSRRGLGILPEWTTSQHLHSSAV
                     LWINAETLRNRRILSLMPLLKGKRGVILQGPGIPFLPSVFREAGITHLVLPAAEEAAA
                     PETRRYIAAGGTPWTCPSLRWRVFPLLYTDEKNR"
     gene            5648..6322
                     /gene="rsmG"
                     /locus_tag="PK442_00125"
     CDS             5648..6322
                     /gene="rsmG"
                     /locus_tag="PK442_00125"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOO86085.1"
                     /translation="MTESVESLVCVHEKKLREYVSLLSSYEGKIRLTGPSDPEVLWNE
                     HIVDCLHSVPYLPESGRTLDVGSGGGLPGLVWAPCRPDLQVTLLDSVRKKCGALAEMA
                     SALQLENVQVVCARCEEYAVKEREVFQCAAARAVAAADVLLEYLAPFVTVSGRVLAFK
                     GPLYVEEIKPMEGKWARLGLSAPEVHTYTAGEKERYLLLWTKQTPVPQRFPRKIGMAE
                     KTKWWR"
     gene            6330..7100
                     /locus_tag="PK442_00130"
     CDS             6330..7100
                     /locus_tag="PK442_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO86086.1"
                     /translation="MITLAISNQKGGVGKTTTCINLAAELGRKGYSVLSVDIDPQANC
                     TSGLGVALPPKAPSLYNVLLGDVPPQEAILQTKWKGVSMLPATLDLAGAEIELASSIS
                     RETKMRRVLSRIEGFDIAILDCPPSLGMLTVNALVAADKLVIPIQCEYFALEGLGQLS
                     RTISLVKDGLNQNLGIDGILLTMHDSRTRLSIDVADEVRRQFGDVVFKTFIPRNVKLA
                     EAPSYAEPICYYDPSCSGAKAYEEFAEEVSTLWLKEED"
     gene            7079..7969
                     /locus_tag="PK442_00135"
     CDS             7079..7969
                     /locus_tag="PK442_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOO86087.1"
                     /translation="MAKGRGLGKGLASLLPAAQTLGEGEPEITMLSMDRILANPFQPR
                     KEINENTLVELADSIRAHGVIQPLIVRRNEDGDYWIVAGERRWRAAKIVGLSEVPVRV
                     IDGNEMEMREITLIENIQREDLSPVDTALALEELLNVYDITQDELAASIGWSRTSVTN
                     KLRLLQLPEEVKTLLSNNELSEGHCRALLPLESQDEIAKLASDAVNAGLSVRQVEDIV
                     KRKNAHSPKEKRPKDVFALPESISQKARSLGFSIRITGKKSKMKLHIEGVHEELVRDV
                     LTYLEENAEKYFPGNTPDEQ"
     gene            8226..8759
                     /locus_tag="PK442_00140"
     CDS             8226..8759
                     /locus_tag="PK442_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOO86088.1"
                     /translation="MFALYVSRETLGVEGGCAVENIFAPWRMAYIAVSKDQGGCIFCD
                     FPKEDNDEARMILFRGETCFVIFNAFPYNPGHLMVAPYRHTAEYELLTDEELLEMHRL
                     AGKCIVVLRKTMNPQGFNLGVNLGKVAGAGFDGHLHLHIVPRWNGDTNFMPVFADVRV
                     VAEELAETYRKMKEAWD"
     gene            8759..9379
                     /locus_tag="PK442_00145"
     CDS             8759..9379
                     /locus_tag="PK442_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOO86089.1"
                     /translation="MQVDYKEPASETTVEVKINKSRFLGHVRICRSEEEVRMKLKEVC
                     DLHKQATHNCWAYRIGEESYREYFSDDGEPAGTAGKPILGAISRLDLTNALIVVTRYF
                     GGIKLGVRGLIEAYGSTATNALNASGMAECTIKSQYQIVAPYDMVKTLHRLLFSLGAV
                     EKDVLFDYGERVSILCSLPRSSGKDFESALDELLNMSRIHSWNGVQ"
BASE COUNT         2440 a         2692 c         2872 g         2229 t
ORIGIN      
        1 cgtcatagca taactccttt cggtaacgga agttccgtgc gaatgatacg aaaacgtatc
       61 ggttgtatct ccgtacgtcg actccaaccg tataagtata cctcttttgc agccgctcag
      121 tcgttttccg tcgagccggg tttgatgatg gacttcatga tgttctccag aagctgtttt
      181 tttacttgct cctccgtgct cgccgaggtg tccgaccccg accccgtggg gatctccaca
      241 gtaatcttta tctgcgtttc gttcttgccg gtggagccgt tggacctcgg aatggtcagc
      301 ggagcgctcc cccgggatac cttcaggtcg agcatctccg gagagtccca cgtccccttg
      361 aggttgaacg aggtctcccg gaagtcgcgg atcgccattc cgccgacgag ccccgtcagg
      421 aagcgctgca agaactgggg gtccgtcagc ggattgccgt cgatggtcag aaacccctga
      481 agagcgccga taaaagcgtt gagcgccctg atattgatgt cgccgacgca tttcaagtcg
      541 aggggggagt cgttccaccc tacggagccg ctcgctgaga agtagcggta gacggtgtct
      601 cccggaggcg cggagagtct gctgccgggg agaagaaaga tgctgcgtcc gtccatgttg
      661 aagcttgcga ggagcgacga gaatcggatt tcccctgttt tcgacaccga tttgatcgac
      721 tcgaaccccg agacggaacc ctccttcgcc gaaatctgcc cgctgccgaa gacgagtccg
      781 agcatgccgc ccgtgccgct cccccgcatg gtgaggtcgg cctttccgga gatcttcccc
      841 tgcccttcga ggatgcgttc cgaagcgctc gcaaggtcca tccccttcac ggcgaggttt
      901 ccctcccacc gcatggtgac agggtcgact tttccgttga agagagcggt tccctcgtgg
      961 tagaccccct tgccccccga aatcacgaac atgccgtcgc tccagcggaa cgggacggag
     1021 acactgcgcg cctccgtttt ataaagtccc agcgattggg cggaaatgga gccttcaaac
     1081 gtcgggacgg cgcccagctt gcctgtgagc gaaaagtccg cgtctccggt gaagatgcct
     1141 gcagcgtgcg ccgaaagcgc ggatgccgtc ttcgcaaggt cgatcttttt cgtgttcttg
     1201 agcgagaatg ttgcacccga gggcgaaacg accgctcgtc cttcaagcgc gagggcgctc
     1261 tccccgagca ttccttcaag agcgacgtcg aacgcgtcct ctccgtccgg cgtgagcgag
     1321 acttttgcct tctcaagcaa cactccgtag acggagagaa agggcgacgt gccggagaga
     1381 gtcgacgtgt acgcgcctcc cgtttttccg gagacgttga gttgcgccga gagcgttccg
     1441 ccgagacgta ccccgggaaa gaacgccgcg gcgagcgaat ggagatcgag cttgttcgcc
     1501 gctatcttca gatttgtctt ttctcctttg ctcaggccga taacacccga aagatcaaca
     1561 gaagactcct gcagtctggc cgagaaatcc gtgatcttca aagatgagag cgttccctcg
     1621 gcctttgccc ggacagccgt caggggcaag gaggcaacgc cgagcgaggg agcggacgcc
     1681 gtcagcgaca gcaaggggga ggatggcttc cccgaaaggg agaactctcc ggcgaccacc
     1741 ccgtccgcgg tctccgcatc agcgcctgcc gcggctgcta tctgtccggc ggagagccct
     1801 ctaaaggctc ctttgaagga gagcgacggc gccgagccga aacgcgcttt cccctcaata
     1861 ttcgccgttc cgccgaagag agagcccgag cctttggaaa cggaaagctc attcttctcg
     1921 aagcgccctt cgagctggat gtttctgaga gggagcgagc gggagaacgg cgtttctctg
     1981 cctttgaggc cgaacgagac gaccggagag gctgtcggcc ccttcacgct ccatgaggcg
     2041 tcgaagaggc ccgcggtctc aagaccgccc agggcgtccc gcagaaggtt ccacgcgagg
     2101 tcgcgtccgc ttccctccat ggtgatggac ggcgactttc cggagagtcc ggtaatgctc
     2161 cccttgccgg agagaagccc ctttccggcg ggagagaaag agagcgcgga gagagaaaga
     2221 atctcttcag tacctgaaaa agctacggaa agcttctctc ctgccgtacc cagaaacgaa
     2281 gcgcggggcg cggcaagagc gcctttgtac gatgcggctc cctccgaagg tatggagatt
     2341 tcaagagcca tcgtaccttt cccatcggcg gagagctcgg gagcgtagac ctttccggag
     2401 gacttcaggt cgaagcttcc ggagtgaaga gaaatggagg tctctgcgcc tgccgagtcg
     2461 tcaagaagac tcttccccga gatggtgaaa ggggaatcag accacgtcgc attcccggag
     2521 atggcaatgc gctccccgtc gaacatgcga agatctccgc ggagcgcgcg gacgtcgaag
     2581 ccttcaagag tcgccttcgg cgcttcgaag agaataggtc cgttcagctt ctcgaaagga
     2641 ccggaaaggt cgattttgag cgtcgagagc gttccttggg cgaaggagag ccaggagaag
     2701 cagtgcgtcc actcttcaat cttcagctgt ttcagtgcaa acgcaatctc cgccgtcggt
     2761 tcctcgccga agtagagggc gaagacgccg gatgcgggcg aggaaaaaac acgtgcgtcg
     2821 acgggagcga caacggcttt cgactcgtcc gcggaccatg agaacgtacc gtccatcagc
     2881 agatcccaga tatcgccgtt ttcgatctcc ccctctccgg agacgagcaa acgtccctcg
     2941 gggcgttcga gcaggagtgt ggagtcgacg gttccctttg cagtaaaggg aaggttcgtc
     3001 agttccgaaa tgacgtccag ccgaagatcg tcgatgtttc cctcgatgcg cgtctccgga
     3061 aaaagcggcc cggagaagga gacaacggcg tttcctcctc tgagaaaacc gtcggtgatg
     3121 gaaagctgtt cgccggtggt aaacgaggcg cccgcctcga ccggaaaatc aaggaaatgc
     3181 ccctctccgt gaaaggtcat tgtcttctcc cccggcgtca ggcggagcga agccagcgag
     3241 atctctccca agggggtcgt gatggttacg ggagagaaca ggatgaccgg aaccatgaag
     3301 tctgtggtta tgccgcttcc ggcggagaag tccgagcgac ggagcgcgtg gagcatcccc
     3361 tctcctgaga ggacgccctc cgtcacggtc acctttgtca gaccgctttg gccacgaaaa
     3421 aacgagagaa tggaaagccc tacggaaagc tcttccgcgc gggcaacctc ctgcccttcg
     3481 aagagaatgc ggatatttgc cgcgttgtac cctgcgagga aatttcctga aataccatca
     3541 gtctccaacg tccatccggc gagcgtggcg acggctttct caagttccgt cttgaaatag
     3601 gacgtcccgg catcctgccc ggagatcagg aagaatgccg caaggaaagc ggcaatacag
     3661 agaacaaaca ataccccagc aatttttttg aatttcccgc gcatacgctc taccctttcc
     3721 cggagcacac cctcggcgtg caatgtaaaa accatactat aatatgcaat cacacggggt
     3781 aattgcaagg caatactact gaaaagagga tgaagagaaa aaagcgcgcg ctgtacaacc
     3841 tatatattga gcctctaagg gggcgggggt attgtggata tgtggaaaaa actgtggata
     3901 acgggtgaaa aacagggcga aagaacaggc ctcgcgcgat tttgtggata acatggactt
     3961 ctgtggatag aaatcacggc acactacggc tcgggcgcag cgcgttactc ggtactccgt
     4021 gatacctgaa tttctgaagc gacagggagt tcgcacccgt tgtacgagag aaggagcttt
     4081 ccgaccgcgc ggatacggtc tccctcgcga agcgagagca gactctcggt ctcgggactt
     4141 ttgtaggcga cgactgtgac gcgtccctcc cgcaggagga tgcgccgccc cttgagttcg
     4201 gccttttcaa tcgtcatgaa gagagtgaga aacgaaccgg caagcaacgg cgcttcgctc
     4261 cacacctgcg ccgaggtaaa caccctccct tccgccctgt tccaaaggcc tctttggccg
     4321 gcacgcgcct cctcgaacgc cttgaggatt cgccccgcgt acctccggtt cggaggaatg
     4381 atgagaggtt gtgcaagtcc ctcccggacg agtatctcgt taagaaacag ttcagagccc
     4441 tcaccaccgg cgaagacgta ggcaagggtg cgtccgtacc tgtccgtccg ttcgctgtcg
     4501 tactccaaac gaagaggtcc gcttgcaagc agtctgctgt tcagcaggcg cgcttcctct
     4561 ccaagctctt ctgccggacg ggacgggtga tgcagttccg gcgtatccac cagaaggtat
     4621 cgtactcgtg cgacgccccc tcccgaagga agctccgcgg tatctccgtc gaagaccctg
     4681 acccccggag gcggaaaagg ctgctcgtct cgctgaaaag gaacggcgcg aaaaaagaga
     4741 aagagaacaa agaacagcgc aaaaaggaga aggaacaagg cgattcgtct catctgtttt
     4801 tctcatccgt atataataga gggaagactc tccagcgaag ggaagggcag gtccacggag
     4861 taccgcccgc tgcgatgtat cgccgcgttt ccggagcagc cgcttcctcc gcggcgggaa
     4921 ggacaaggtg cgtaatgccc gcctctctga aaaccgaggg aagaaacggt attcctggcc
     4981 cctggaggat cactcctctc ttccctttca gaaggggcat aagcgagagg attctccggt
     5041 tgcgcagcgt ttctgcgttt atccagagta cggcgctcga atgcaggtgc tgcgatgtcg
     5101 tccactcggg aagaatacca agaccgcgcc gggagtcgtc gaagatggaa atcttccagt
     5161 tccagtcgcg caaaaagtct gtcacccagg ggtcaggggc aagaagggcg acccggtccc
     5221 tcccggggag ctgcagaagg aattccaaag gttcgccccc ctcaggcgcc ggggcgagcg
     5281 cggcgagcga ggccgacgca gcggaaaact cctgcggata cggggagacg aggagccggg
     5341 cgagactatg ggcgccggac atgagaatgt tgcgcgtatg ctcttcccgg ggggacatcg
     5401 gctcgcctcc ctcgggcgga gtggaaccga cgccccatcg gccgtccgaa agcgagagaa
     5461 aactcccctt ccagccgatg atcaaattgt cgacggctcc gtcgggagct ttcaggactt
     5521 cttgtagaat ggaatcatac aggcgcatat cgctcctcct cgccgaccat tgtaacgcat
     5581 acgaggagag aagcagaagc gctactcttt gaaaactcaa attctgaacg tcgaaaggag
     5641 aagcgtattg acagaaagtg ttgaatcgct ggtgtgtgta catgagaaga agctgaggga
     5701 gtatgtatcg ctcctgtcct cgtacgaagg caaaattcga ttgacgggac cgagcgaccc
     5761 cgaagtcctc tggaatgaac atatcgttga ttgcctccac tccgttccgt atctaccgga
     5821 atcgggaaga acgctcgacg tggggtcggg aggaggtctt cccggtcttg tgtgggctcc
     5881 ctgccgcccc gacctccagg tgacgctgct tgacagcgtt cgcaaaaagt gcggcgcgct
     5941 tgccgagatg gcctccgcgt tacagctgga gaatgttcag gttgtctgcg ctcgctgtga
     6001 agaatacgcc gtgaaggaac gggaggtttt tcaatgtgca gcagcccgtg ccgttgccgc
     6061 agcggatgtg ctcctggagt atcttgctcc ctttgtgacc gtttcaggac gtgtgcttgc
     6121 attcaaagga ccgctctacg tagaggagat caagccgatg gaaggaaaat gggcgcgcct
     6181 cggactctcc gcaccggaag tgcacaccta tacagccggt gaaaaagagc gctaccttct
     6241 tctctggaca aaacaaacgc ctgttcctca acgatttcca cggaagatag gcatggcgga
     6301 aaaaacaaaa tggtggaggt gatgcgggag tgattactct tgcaatatcg aatcagaaag
     6361 gcggagtggg aaagaccaca acatgcatca atctcgctgc ggagcttgga cgcaaagggt
     6421 attccgtcct ctcggtcgat atcgacccgc aggcaaactg tacgagcggg ctcggcgttg
     6481 ccttgcctcc gaaagcaccc agcttgtata acgtcctcct tggcgacgta ccgccgcagg
     6541 aggcaatcct tcaaaccaag tggaagggtg tttcaatgct tccggcgacg ctcgacctgg
     6601 cgggggcgga gatagagcta gcgagttcta tcagcagaga gacgaagatg cgtcgtgtgc
     6661 tctcccgaat tgagggcttc gatatcgcaa ttctcgactg tccgccttca ctcggcatgc
     6721 tgacggtcaa cgcgctggtc gccgccgata agctggtcat tcctatacag tgcgagtatt
     6781 ttgcactcga agggttaggg caactctcca ggacgatatc gctcgtaaaa gacggattga
     6841 atcaaaacct gggcatcgac ggaattctct tgaccatgca cgactcaagg acgcgtcttt
     6901 ctatcgatgt ggctgatgaa gtccggaggc agttcggcga cgtcgtcttc aaaacattca
     6961 tcccgaggaa tgtcaaactg gcggaagctc caagctacgc tgagccaatc tgctattacg
     7021 acccctcatg ctcaggtgca aaagcctatg aagagttcgc ggaggaggtg tcgacgctgt
     7081 ggctaaagga agaggactag gaaagggtct tgcgtcgctg ctcccggcgg cgcaaacgct
     7141 cggggaggga gaaccggaaa tcacaatgct ttcgatggat cgaattctcg ccaacccgtt
     7201 ccagccaaga aaagagatca acgagaacac tctggttgaa cttgccgact ccatcagggc
     7261 gcacggagta atacaaccgt tgatcgtccg gagaaatgaa gacggcgact actggatagt
     7321 cgccggagag agacgatgga gagccgcgaa gatcgtaggc ttaagcgaag ttcctgtgcg
     7381 cgtgatcgat ggaaacgaga tggagatgcg tgaaatcaca ctcatcgaaa acatacaacg
     7441 cgaagatctc tcaccagtgg acactgcgct cgcactcgaa gaacttctga acgtctacga
     7501 catcacacag gatgaacttg ccgcaagcat tggatggagc agaacgtccg tcacaaataa
     7561 gctccgcctt cttcagttac ctgaagaggt taaaacgctc ctctcgaaca acgagctctc
     7621 cgagggtcac tgtagagcgc ttctcccgct ggagtctcag gatgagattg cgaaacttgc
     7681 ctcagacgca gtgaacgccg ggttatctgt gcgtcaggtt gaggacatcg taaaacgaaa
     7741 gaacgcccac tctccgaaag aaaaaagacc aaaggatgtc tttgcgcttc ctgaaagcat
     7801 ctcccaaaaa gcgcgatctc ttggattttc aatacgaatc acggggaaga aatcaaagat
     7861 gaagctgcat atcgaaggag ttcacgaaga gcttgtccgg gatgttctca catatcttga
     7921 agagaacgcg gagaagtatt tcccgggaaa tactccagac gaacaatgaa aaactacgca
     7981 gatctgatcc ttttcggaaa ggtagtatcc acagcactcc tcgtggtagg atatattttg
     8041 ctcggttact atttgggaag aagattggta gataacggat accctacctg gacacacccg
     8101 gcactcatac tggttggagc aatagtcgga acacaccaaa tgtatttcgt cataagggaa
     8161 ttaattcata aaatcaacaa atagaataca gcggtagggt ataaaaacca aacaatataa
     8221 tgtaaatgtt tgctttatat gtttcacgtg aaacattggg tgttgaaggg ggttgcgctg
     8281 tggagaatat ttttgcgccg tggagaatgg cgtatattgc tgtttcaaag gaccaaggcg
     8341 ggtgtatttt ttgtgatttt ccaaaagagg ataacgatga ggcgcgaatg attcttttca
     8401 gaggggagac gtgctttgtt attttcaatg cttttccata taatccgggg caccttatgg
     8461 tcgcccccta taggcataca gccgagtatg aattgttgac cgacgaagaa ctgctcgaaa
     8521 tgcatcgtct tgcgggaaag tgcatcgtgg tgctccgaaa gacgatgaac ccgcagggtt
     8581 tcaatcttgg cgtgaatctt ggaaaggtgg cgggggctgg ttttgacggt catctccatc
     8641 ttcacattgt tcctcgatgg aacggagata cgaattttat gcctgtattt gcagatgtac
     8701 gagtagtcgc ggaggaactg gcagaaacat acagaaagat gaaggaagct tgggattgat
     8761 gcaggttgat tacaaagaac cagcttcgga aactacggta gaagtaaaaa tcaacaaatc
     8821 cagattcttg ggccatgtgc gtatttgcag atccgaggaa gaagttcgga tgaagctcaa
     8881 ggaggtgtgc gatctgcaca agcaggcaac gcataattgc tgggcataca ggatcggcga
     8941 ggagtcatat agagagtatt tttccgatga cggcgaaccc gcagggacag cggggaaacc
     9001 gatccttgga gcaatttcac gacttgatct gacaaatgct cttattgtag tcactcgata
     9061 tttcggaggc ataaagcttg gtgttcgcgg tttaatcgaa gcgtacggct ccactgcgac
     9121 gaatgcgctg aatgcctccg gtatggcgga atgtacgata aagagtcagt accagatagt
     9181 tgctccgtac gatatggtaa aaacattgca ccggctgtta ttttcacttg gcgcagtcga
     9241 gaaagatgtt ctcttcgact acggagagcg tgtcagtatt ctttgttcgc tgcctcgatc
     9301 ctccggaaag gattttgaaa gcgcgcttga tgagctcttg aacatgagca gaatacattc
     9361 atggaacggt gttcagtaga cttcttcatt cttccgggcg ccgttccgtt ttttttgcca
     9421 ccctttcaat ctttgagaag atctgattaa agggtcttct ttcttcatcg agctcgattg
     9481 gaacgagttc ctacgagtct gactcgacag tattctgggt ttcctttgtt gaagaatcac
     9541 atttttcgcc gcgaattctc tggagagtaa ctttctacaa cacatgagtc tttttcatat
     9601 atcgcttggt tttggtacac taagattgat cactttttga gcgaacgaca gccgaaaatt
     9661 gatcatctga cagcagctcc ggttatcaac gctttctcct tttcgtattg agactcagca
     9721 aagagtctca atacgaaccg ttttcattta agaatgagtt ccaggaattg tagtccatat
     9781 ttggaatgga atcacggaaa aacaaactcc ttcctcgaac tcgtaaaaag tagatttctt
     9841 tagttgttcc cttcgccttc gatactcgag ctcaccacta aacataattc aaccacaatg
     9901 ccactttcgt gtgccttctt ccggatgtcc gaagtgcatc gtcgtattta ttttgattaa
     9961 gagccaaaat tctttcgttc ttttcatcga aatctcctgg tttgttccct ttcgaaactc
    10021 ttcttgttga atgaaacagt tctgatagaa ccaaagtgga tctcttggaa agaatctttg
    10081 tttcttctct ttctggttga tgatgttatg agtcaatgag acagtctgat tcaacgcgcc
    10141 acacgtccac atttcgtgtc tggtcgaaat gcgtgcatct ctgttcctta ctcatcgtat
    10201 tgagaagtag tggtggaatt cttgtacacg ccc
//
LOCUS       DAOSWW010000004        10214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_4153_length_10214_cov_3.900975, whole genome shotgun sequence.
ACCESSION   DAOSWW010000004 DAOSWW010000000
VERSION     DAOSWW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4153_length_10214_cov_3.900975"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PK442_00150"
     CDS             <1..356
                     /locus_tag="PK442_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86090.1"
                     /translation="QNGTCEYFPLVVNPDKWNALPQNYKDIVMVARDRIRYLSAMVYV
                     HEIKSREKWKTMPNLQIVSWSLEDQKKARAIGQQLVLEECNKTPEGKEYLEIYRKTLW
                     ELGHKEEAKNLGYAE"
     gene            complement(441..1121)
                     /gene="rlmB"
                     /locus_tag="PK442_00155"
     CDS             complement(441..1121)
                     /gene="rlmB"
                     /locus_tag="PK442_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOO86091.1"
                     /translation="MQKVYLGKNVSDRFSLQIGDLAEKGKVTVLRVTPEALTDMCGTN
                     NHQGVACRSGDVSMLELQDILPLLDGKKGPVLLVLLDHVQDPHNLGAVVRTAEACGAM
                     AVIFPKRRAALPGGTVVKVSAGAALRVPMVAVTNVAQTVREIQQNGFWTIGLDNKNGR
                     SLWSETMPEKTLLVVGAEGEGLSRLVAETCDETVRIPVAGATGSLNASVACAVGMFEW
                     ARKWGAQS"
     gene            complement(1180..4125)
                     /gene="uvrA"
                     /locus_tag="PK442_00160"
     CDS             complement(1180..4125)
                     /gene="uvrA"
                     /locus_tag="PK442_00160"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOO86092.1"
                     /translation="MSQWIHIKGAREHNLKNVDVDLPREQLIVITGPSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGVQKKPDVDDISGLSPAISIEQKGTSHNPRSIVGTV
                     TEVYDYLRLLFGRVGTPYCPSCGKPVLRHTLDEIINYLLQDFSEKRLEILAPLVRGKK
                     GEFRNLFAQTREKGFLRVRVDGAVLWLEEEISLDKNKRHSIEAVIDRLRIREDRRSRI
                     AEAIETAFSLSDGYVLLLDEEGNERVLTEKFACADCELTFPEIEPRLFSFNTPYGACP
                     DCSGLGSHQYFAEELAVDPGRTITDGAILPWKKKHYMLTKLEKFAKHNDWDLSSPYGE
                     LPEHVRSFILYGTDERIPLTFRENGEEKSYMGRYEGLLNWLESRWKETESEGVIEELE
                     TYRADDLCQSCKGLRLRPEALYVRLGGYGIGDLISMPVDSLRQTLETISFTSSEEHVV
                     HQVLLEIKKRLDFLSDVGVGYLSLLRRADTLSGGESQRIRLATQIGSKLSGVLYVLDE
                     PTIGLHSRDTEKLVRTLEAIRDLGNTVVVVEHDRETMTAADSIIEMGPGAGEGGGSIV
                     FSGSYEDALHSTHLTGPYLRGERTAVIGEGRRTPKGWLTVKGASHNNLQGIDVSFPTG
                     VFVSVTGVSGSGKSSMVHDVLYKGLRRLLDRDFRERAGNHLTIEGSEQFRNVILVDQS
                     PIGRTPRSNPATYTGLFTLIRELFSGLPESRLRGFAPGRFSFNVKGGRCEACGGGGVT
                     KVSMLFLPDVYVPCEVCGGKRYNHETLEVKFKGKSIADVLDMTVDEAYAFFRDIPRIA
                     SKLQLIAEAGLGYIRLGQSALTLSGGEAQRVKLSKELSKRFSGPTLYLLDEPTTGLFY
                     TDVEKLLHIINKIVDNGNTVILIEHNLDVLLSSDYIVDLGPEGGSGGGRLVATGTPED
                     IMTGEKGHTAYYLQEYAKQIAERVKKSGKKNGPNSGQKSSKRAEKRGETELSQISSTE
                     RRTKRREAK"
     gene            complement(4064..6097)
                     /gene="uvrB"
                     /locus_tag="PK442_00165"
     CDS             complement(4064..6097)
                     /gene="uvrB"
                     /locus_tag="PK442_00165"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOO86093.1"
                     /translation="MFIEKKFELHSDWAPSGDQPQAIETLAAGVAQGLRFQTLMGVTG
                     SGKTFTVANVIASAQRPVLVLAHNKTLAAQLYSEFKAFFPRNAVHYFVSYYDYYQPEA
                     YVPSTDTYIEKDASVNERIERLRLAATKALIERRDVIVVASVSCIYGLGLKEAYESVI
                     FPFAVGEQWERRAFLEKLLENYYDRNDIVLEPGKFRARGDIVEIFPAYGERALRIAFF
                     DEEIERIDEFDPVSGKVIENLEKASIFPAQHYVTDRDAISNAVEPIQTEMELQAAEFE
                     KQGKFLEAQRIRMRTLYDLEMLRETGYCSGIENYSRYLDGRNPGEPPGTMIDFFPEDF
                     LLVVDESHITLPQVRGMFNGDRARKLTLVENGFRLPSCLDNRPLEWKEFEQHINQAIF
                     VTATPGDYEMQVSGKMVEQVIRPTGVVDPEVEILPAKGQVDDLISRLRDIVRNGQRAL
                     VTTLTKKSSEDLAEYLAELRLKVKYIHSELNAFERAELIRDLRTGDISVLVGINLLRE
                     GMDLPEVALVAILDADREGFLRSYRSLIQMMGRAARNICGKVILYADEITESIRKSVD
                     ETVRRRRLQMKYNEENNITPITIQKEIVHLLPEELLEDQPTGKGRGGAETFKELSVPD
                     MEKMMWQAVERLDFEKAARIRDALASLEGNEWNRVAMDTHKRSTRTQSKKRRR"
     gene            complement(6196..8121)
                     /gene="ftsH"
                     /locus_tag="PK442_00170"
     CDS             complement(6196..8121)
                     /gene="ftsH"
                     /locus_tag="PK442_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOO86094.1"
                     /translation="MGRLVKNLGLYLILIVLVVSLVNVFLSPTQGPQQVQEIGYSTFL
                     SEVTAGRVSNVLVRENSIKGRFSDGKEFVSYVVGIGDLAKEVAQKGVAVEVEPPQRTP
                     WWANMLSSLFPTLLLIGVWIFFLYNMQGGGGKVMNFAKSKAKLFIDNRPKVTFKDVAG
                     CDESKEELSEVVYYLRDPSRFTALGARVPRGVLLLGPPGTGKTLLARACAGEADVPFF
                     SVSGSDFVEMFVGVGAARVRDLFEQARKYQPCIIFIDEMDAVGRHRGAGLGGGHDERE
                     QTLNQLLVELDGFDETTGIILIAATNRPDILDPALLRPGRFDRHIVVDRPDVKGREAI
                     LDVHVREKKLANDVDLAVLARRTPGFVGADLANLVNEAALLAARTGEKEIKMKDFEEG
                     IDRVIAGPERKSRLVSDKEKLIIAYHETGHALVAKRIPSCDPVHKISIIPRGHMALGY
                     TLQLPEEDRFLMSKNELLDKICVLFGGRVAEELRFGDVTTGASNDLERATQIARQMVT
                     EFGMSEKLGLVKLGHKHNEVFLGRDIGEERNYSDAIAYAIDQEVKAIIDSCYEKVRNI
                     LVENSEEMEKIAKTLLEKEIIEGKELNALLGLENPEEEKVVGPEEEERTETQETIAEE
                     NPVGESKNSDAATNAAI"
     gene            complement(8162..8695)
                     /gene="hpt"
                     /locus_tag="PK442_00175"
     CDS             complement(8162..8695)
                     /gene="hpt"
                     /locus_tag="PK442_00175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOO86095.1"
                     /translation="MEYQISAILLSQEELAARVREIGETIGRDYAGKELVVIGILKGA
                     VIFLSDLVRAVPADVNITLDFMAVSSYGDSTKTSGIVRIVKDLDGSIKDKDVLLVEDI
                     VDTGLTLSYLVKMMRERQPKSVRVCALLDKEERRKVHVDVDYRGFPIPDEFVVGYGLD
                     YGGRWRHLPAIYTIEQK"
     gene            complement(8706..9974)
                     /gene="tilS"
                     /locus_tag="PK442_00180"
     CDS             complement(8706..9974)
                     /gene="tilS"
                     /locus_tag="PK442_00180"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOO86096.1"
                     /translation="MLLAVSGGSDSMALMWFFHTLWEGHCIVAHLNHGIRGESADKDQ
                     RFVEEIATRFSFPVESASYPVPELLERGESLEDGARRIRYEFLEKTMKQTGACGVAVA
                     HTSDDSAETFLMNLMRGTGVRGLVGVPELRDSFIRPLLAFSKNYLKEILEFHGIPWRE
                     DLSNRDNAYTRNRVRNSLIPFLQKEFNPRVKEGILGIAEDMKHFRENEEEMQSALVDL
                     LERRVPFTEYACSLQAMRSLDEQTCAVFVRGVGARLGLRTLSRDRTNTLCRLVHKSSR
                     WCFQWQKEIVLFCSFHLVAWIDPAILVQSAGASPSSFALEEQSGFFEWGKWLFSWKRE
                     WASASNYGTMGAVFPNSERIEVVPFALFRKAIRKRDIPEWAVSLFPVVYSDRMQWLPY
                     WRGSGRWESLHDVAIRISAKYLQNIDERKR"
BASE COUNT         2333 a         2750 c         2546 g         2585 t
ORIGIN      
        1 tgcagaacgg tacgtgcgag tatttcccgc tggtggtcaa tcccgacaag tggaatgcgc
       61 taccccagaa ctacaaggat atcgtcatgg ttgcacggga tcgtatccgc tatctttcgg
      121 cgatggtcta tgtacatgag atcaagtcgc gggagaagtg gaaaaccatg ccgaaccttc
      181 agatagtgag ctggagtctt gaggatcaga aaaaagctcg ggctatcggt cagcaactcg
      241 ttcttgagga gtgcaacaag actcccgaag ggaaagaata cctcgagatt taccgcaaaa
      301 cgttgtggga gctcgggcac aaagaagaag ctaaaaacct tgggtacgca gagtaattaa
      361 tcgtaataat cagatgttgt aaaaatgccg gttcagggat agctccccga accggcattt
      421 tttgtgatac tgaaagagaa ctaggattgc gccccccact tcctcgccca ttcaaacatt
      481 ccgacggcgc aagcgacgct tgcgttcagt gaacctgttg caccggcaac cggtatccgg
      541 acggtctcat cgcacgtttc cgccacaaga cgggagagtc cctccccctc cgcgcctact
      601 acaagcaggg tcttttccgg catggtctcc gaccagagcg agcggccgtt cttattatcc
      661 agccctatag tccagaaccc gttctgttgg atctctctga ctgtttgtgc aacattcgtt
      721 actgccacca tgggaacgcg taatgccgct ccggcgctta ctttgacgac agtccctcct
      781 ggaagggcgg cgcgtctttt agggaagatg actgccattg ccccgcaagc ctcggcagta
      841 cgcacaacag ccccgaggtt gtggggatcc tggacgtgat caaggagcac aagaagtaca
      901 ggccctttct tcccgtcaag aagaggaaga atatcctgca gctcaagcat cgatacatct
      961 ccgcttctgc aggccacccc ctgatgatta ttggttccgc acatatcggt gagcgcttca
     1021 ggcgttactc tcaagacggt gactttccct ttttctgcca aatcgcctat ctggagcgaa
     1081 aaacggtcgc tcacattttt accgagatag accttctgca ctttgtcagg agattccttc
     1141 aaaagctgga ggacgacctg ccttccccat tgaatatcct cacttcgctt ctcttcgctt
     1201 cgttctcctc tcggtggaac tgatttgcga taattccgtt tctcctcgtt tttcggcgcg
     1261 cttcgaactc ttttgtccgg agttcggtcc attctttttt ccactctttt tcaccctttc
     1321 ggcgatttgc ttcgcatatt cttgcagata gtatgcggta tgtcccttct ctcccgtcat
     1381 gatatcttca ggggttccgg ttgcgacgag acggcctcct ccgctccccc cttccggtcc
     1441 caggtcaacg atgtagtcgg aggagagcag aacgtccagg ttatgctcga tcaaaatgac
     1501 agtgttgccg ttgtcgacta ttttattgat gatgtggagc agtttctcaa catctgtgta
     1561 aaagagacct gtagtcggtt cgtcgaggag gtaaagtgta gggccgctga agcgtttgct
     1621 caactctttg gagagtttga ctctctgcgc ttctcctccg ctcagagtga gtgcggactg
     1681 gccaaggcgg atatacccca agccggcctc tgcgatcaac tgaagtttgg aagcgatccg
     1741 tggaatatca cgaaagaaag catacgcttc atctacagtc atatcaagga catcggcaat
     1801 ggattttcct ttgaatttta cttcaagggt ctcgtgatta tatctcttcc ccccgcacac
     1861 ttcacacggc acgtaaacgt cggggaggaa gagcattgaa accttggtga cgccgccgcc
     1921 gccgcatgct tcgcatcttc cgcccttgac gttgaaactg aatcgccccg gcgcaaaacc
     1981 gcgcaggcgc gattcgggaa gccccgaaaa gagctcccgt atgagtgtga aaagtcccgt
     2041 atacgttgca gggttagaac gtggggtccg ccctatagga ctttggtcga caaggatgac
     2101 gttcctgaac tgttcgctcc cttcgatcgt cagatgattg ccggcccttt cccggaagtc
     2161 cctgtccaaa agccttctga gacctttgta gagtacgtca tgtaccatgc tgctctttcc
     2221 cgagccggag actccggtga ctgagacaaa gacacctgtc gggaacgaaa cgtcgattcc
     2281 ctgtagattg ttgtgtgatg cgccctttac tgtcagccag cctttgggag tccgtctgcc
     2341 ctcccctatc acagcggttc gctcgccccg aaggtaaggg cctgtcagat gtgttgaatg
     2401 caaggcgtct tcatagcttc cggagaagac gatactgccg cccccttctc cggcgccggg
     2461 ccccatttca atgatcgaat cggccgccgt catcgtctct ctgtcgtgtt caacgacgac
     2521 gacagtattg ccaagatctc ggatggcttc aagtgttctg acgagttttt ccgtgtcccg
     2581 tgagtgaaga ccaattgtcg gttcgtcaag aacgtagaga acgccgctca acttcgagcc
     2641 tatctgagtg gcaaggcgga ttctctggct ctctcccccg ctgagggtat cggcgcggcg
     2701 tagcagagaa agatagccca ctccaacatc ggagaggaag tcgaggcgtt tttttatttc
     2761 cagcaatact tggtgtacga catgttcctc ggaagacgtg aaggagattg tttccagcgt
     2821 ttggcggaga gagtcgacag gcattgatat aaggtcgccg ataccgtatc ctccaaggcg
     2881 gacgtaaaga gcctcggggc gcagcctgag ccctttgcag gattggcaaa gatcatctgc
     2941 gcggtatgtc tccagctctt cgattacgcc ttcggattct gtttccttcc agcgggattc
     3001 aagccagttc agcagccctt cgtaacggcc catgtagctt ttttcttcgc cattctctct
     3061 gaacgtcaaa gggatacgct catcggttcc gtagagaata aacgaccgaa cgtgttcggg
     3121 caattccccg tagggcgacg aaagatccca gtcgttatgt ttcgcaaact tctccagttt
     3181 tgtgagcata tagtgttttt tcttccatgg caaaatggct ccgtcggtga tagtccttcc
     3241 tggatcaact gccagttcct cggcaaaata ctgatggctc cccaggccgg aacagtcggg
     3301 gcaggctccg tagggcgtat tgaaagagaa aagtctcggt tctatctccg gaaaggtaag
     3361 ttcgcagtca gcgcaggcaa atttctccgt gagaactctt tcgttcccct cttcgtcgag
     3421 gagaagaacg tatccgtcgc ttaaggagaa tgcagtttca atggcttcag ctatccgtga
     3481 acggcggtcc tcacgtatac gaagacggtc gatgacggcc tctatagagt gtcgcttgtt
     3541 cttatcgagc gaaatttctt cttcaagcca gagcacagcg ccgtcgacac gaacacgaag
     3601 aaatcccttc tcccgcgtct gggcgaagag gttgcgaaac tcgccttttt tccctcgaac
     3661 aagcggggca aggatttcca aacgtttctc ggagaagtcc tgcaggagat agttaataat
     3721 ctcgtcaaga gtatgacgaa gaacaggctt cccgcacgaa gggcaatacg gcgtccctac
     3781 tctgccgaag agaagccgga gatagtcgta cacttccgta accgttccta cgatcgaacg
     3841 aggattgtgc gacgtccctt tctgttctat tgatatggcg ggagaaagtc ccgaaatgtc
     3901 atcaacgtcg ggcttttttt gtactccaag aaactgtctc gcgtatgctg aaagagactc
     3961 gacatatctt cgttgccctt ctgcatagag cgtatcgaag gcaagcgacg attttccgga
     4021 accggacggt cccgttatga cgatcagctg ctcgcggggc agatcaacgt ctacgttttt
     4081 tagattgtgt tctcgtgctc cttttatgtg tatccattgc gacacggttc cattcattcc
     4141 cttcaagcga agcaagggcg tccctgatgc gggccgcctt ctcaaaatca agccgttcaa
     4201 cggcctgcca catcatcttt tccatgtccg gtaccgagag ttctttgaac gtttccgctc
     4261 cgccacggcc tttccccgtg ggctgatcct caaggagctc ttccgggaga agatgtacaa
     4321 tctctttttg tattgtgata ggtgtaatat tgttttcttc attatacttc atctgcagtc
     4381 tgcgccttcg aactgtttca tccaccgatt tcctgatact ctcagtgatc tcgtcagcat
     4441 agagaataac cttgccgcag atgtttcgag ccgcccgccc catcatctga atgagcgagc
     4501 gataggatct gagaaagcct tccctgtctg cgtcgagaat ggcaacaagc gcaacctcgg
     4561 gaaggtccat accttccctc aggagattaa tgccaaccag aacggagata tcgcctgtgc
     4621 ggagatctcg aatgagttcc gcgcgctcga aggcattcag ttccgaatgt atgtacttta
     4681 ccttgaggcg aagctccgca aggtattccg cgaggtcttc tgaggacttc ttcgtgagcg
     4741 tggtgacaag agcccgctgt ccgttgcgga cgatatcgcg cagacgggat atgaggtcgt
     4801 caacctgccc cttggcggga agaatttcta cttcgggatc aacgacgccc gtggggcgta
     4861 taacctgctc caccatcttt ccggaaacct gcatttcata atcgccggga gtagccgtaa
     4921 cgaagatcgc ctggttgata tgttgctcaa actccttcca ctccaaaggc cggttatcca
     4981 gacacgatgg aagacgaaac ccgttctcaa cgagcgtgag ctttcttgcc ctgtcgccgt
     5041 tgaacatccc ccgtacctga ggcagcgtta tatgcgattc gtcgacgacc aataaaaagt
     5101 cctcggggaa gaaatcgatc atcgtccccg ggggttcgcc cggattcctt ccgtcaagat
     5161 aacgggagta gttttctatc ccggagcagt acccggtttc acggagcatt tcaagatcat
     5221 agagagtgcg catacgaatc cgctgcgcct caagaaactt gccctgtttt tcaaactcag
     5281 ccgcctgaag ctccatctcc gtttgaatcg gctccacagc attcgagatg gcgtccctgt
     5341 cggttacgta atgctgcgca gggaatatgg aggccttttc cagattttca atcacctttc
     5401 cggagacggg gtcgaattcg tcgattcgtt caatctcttc atcaaaaaaa gcgattcgca
     5461 gggctctctc tccgtatgcg ggaaaaattt cgacaatatc acccctggct cggaatttgc
     5521 cgggttcgag gacaatatca ttcctgtcgt agtagttctc cagcagcttt tcaaggaaag
     5581 cgcggcgctc ccactgttca cccactgcaa aaggaaagat gacgctttca tacgcttctt
     5641 ttaaaccgag accgtatata caggaaacgc tggccactac tatgacatcg cggcgttcaa
     5701 tgagcgcctt tgttgcagca agtcgcagac gctcaattct ttcatttacc gaggcgtctt
     5761 tctcgatgta cgtgtccgtg gagggaacat acgcctccgg ctggtagtag tcatagtagc
     5821 tgacaaaata atgcactgcg ttccggggaa agaacgcctt gaattcgctg taaagttggg
     5881 cggcaagcgt cttgttgtgt gcaaggacaa gaacaggacg ttgtgcggag gctattacat
     5941 ttgctacggt gaacgttttt ccgcttcctg tcacacccat aagggtctgg aatcgaaggc
     6001 cttgggcgac accagccgcg agcgtttcaa tcgcttgcgg ctgatccccg gagggggccc
     6061 aatcagagtg gagttcaaac ttcttttcaa tgaacatacg gaatccaccc ccaagtgaaa
     6121 gaaaagaggg atccggggag agcgttgcgc ccgttcccgg atccttctga agacacggta
     6181 ttcccagttt ttccgttata tagccgcgtt ggttgcggcg tcgctgttct tactttcgcc
     6241 gacaggattt tcttcggcga tggtctcttg cgtctcagtg cgttcttctt cttctggtcc
     6301 tacaaccttt tcttcttcag gattctccag accaagaaga gcgttgagtt ccttcccctc
     6361 aatgatctct ttttcgagaa gtgtttttgc tattttttcc atttcttccg agttctccac
     6421 aaggatgttt cggactttct cataacagga gtctatgatt gccttgactt cctggtcgat
     6481 agcgtacgcg atcgcgtcgc tgtagtttct ttcttctccg atatctctgc caaggaatac
     6541 ttcattgtgt ttatgtccca gttttacaag tccgagcttc tcgctcatgc cgaattcggt
     6601 aaccatttgt cgggcaatct gtgtcgcccg ttcgaggtcg ttgctcgcac cggtagttac
     6661 gtccccgaac ctgagttcct cggcaactct gccgccgaaa agaacgcaaa ttttgtcgag
     6721 gagttcgttt ttcgacatga ggaagcgatc ctcctcgggg agctggagcg tgtaccccaa
     6781 tgccatgtgt cctcggggga ttatcgatat tttatgaacc ggatcgcatg aaggaattcg
     6841 tttcgcaaca agcgcgtgcc ccgtttcatg gtatgcgatt attagtttct ctttatcgct
     6901 gacgaggcga ctcttccttt ccgggccggc tatgacacgg tcgatgccct cttcgaaatc
     6961 tttcattttg atctcttttt cacccgtgcg tgcggctaaa agcgcagctt cgtttacaag
     7021 gttggcaaga tcggcgccga caaaacccgg tgttcggcgc gcgaggacgg caaggtcgac
     7081 gtcgttcgca agcttctttt cgcggacgtg aacgtcaaga atagcctctc gtcctttaac
     7141 gtcgggtctg tcgacaacga tatgtctgtc gaaacgaccg gggcgcaaca acgccgggtc
     7201 gaggatatcg ggccggttgg tagctgcgat caggatgatc cccgtagtct cgtcaaaccc
     7261 gtcaagttca accagaagct ggttgagcgt ctgctcgcgt tcgtcgtgtc ctccgccgag
     7321 tccagcgcct ctgtgacggc caactgcatc catttcgtcg ataaaaatga tacagggttg
     7381 atatttcctg gcttgctcga aaagatcccg aacacgggcg gctcctacgc cgacgaacat
     7441 ctcgacgaag tccgatccgc tcacgctgaa aaaaggaaca tccgcttcgc cggcgcaagc
     7501 acgggcgagc agcgttttcc cggtaccagg aggtccaagc agcagaactc cgcgagggac
     7561 tctggcgccg agggcggtga aacgggaagg atcccgaaga tagtagacga cttctgaaag
     7621 ctcttctttc gactcgtcgc acccggcgac gtctttaaat gtgactttcg ggcggttgtc
     7681 aatgaagagc ttcgccttgc ttttggcgaa gttcatcacc ttgccgcccc cgccctgcat
     7741 gttgtaaaga aagaaaatcc acacgccaat cagcagcagg gtcgggaaga gtgaagagag
     7801 catatttgcc caccaaggag ttcgttgcgg gggttcaacc tccacggcga cgcccttttg
     7861 agcgacttcc ttcgccaaat cgcctattcc gaccacgtat gagacaaact ctttaccgtc
     7921 ggaaaatctg cctttgatgg agttttcgcg taccaggaca tttgaaactc tcccggctgt
     7981 aacttcggag agaaacgtgc tgtaccctat ctcctgaact tgctgaggcc cctgtgtcgg
     8041 cgagagaaat acgttgacca gactcacgac aagtacaatg agtatgaggt ataaaccaag
     8101 attttttacc aatcggccca aattatagcc gcctcctgac taaaaatagt attgtaaata
     8161 gttacttctg ttcgattgta taaatagcgg gcagatgacg ccagcgtccg ccataatcaa
     8221 gcccataccc tacgacgaac tcgtccggta tgggaaagcc tctgtaatcg acatccacat
     8281 gtaccttacg tcgttcctct ttatcgagca gtgcgcacac acgaacgctc ttcggttggc
     8341 gttcccgcat catcttcaca agatatgaca gggtgagtcc ggtgtcaaca atatcctcta
     8401 ccagcagtac atctttgtct ttgatgctgc catccagatc ctttacaata cgaacaatac
     8461 cgctggtctt cgtggaatcc ccataggaag agacagccat gaaatccaaa gttatgttga
     8521 cgtcggcggg aactgccctc accaagtcgg aaagaaaaat aacagctccc ttgaggatgc
     8581 ctatgactac caattcttta ccggcataat cgcgtccgat cgtctctcct atctcccgga
     8641 cgcgggcagc caattcttcc tgtgaaagga gaatcgcaga aatttgatac tccattgtcg
     8701 ttacgtcacc ttttcctttc atcaatgttc tgcaggtatt ttgcagaaat acgaatcgca
     8761 acgtcgtgca gactttccca gcgaccgctt ccccgccagt agggaagcca ctgcattcga
     8821 tcagaataca ccaccggaaa gagagacaca gcccattcgg gaatatctct cttccggatt
     8881 gcttttctga acagcgcaaa agggacaacc tcaatcctct cgctgtttgg aaatacggcc
     8941 cccattgtac cgtaattact tgcggatgcc cattccctct tccaagaaaa aagccacttt
     9001 ccccattcaa agaaaccgga ttgttcttca agagcaaaag aggatgggga ggcgccggca
     9061 gactgaacta gtatagcagg gtcaatccat gcgacaaggt gaaaactgca gaaaagaacg
     9121 atctcttttt gccactggaa acaccaccgc gaagatttat gaacaagtcg gcagagggtg
     9181 tttgtcctgt cacgggaaag cgttcgaagt ccgagcctgg ctccaacccc tctgacgaac
     9241 accgcacacg tctgctcgtc gagcgagcgc attgcctgta aagaacaggc gtactcagta
     9301 aagggaacgc gtctttccag gagatcgacg agtgcggatt gcatttcttc ttcgttctcc
     9361 cggaaatgtt tcatgtcctc tgctatacca aggattcctt cttttaccct tgggttgaat
     9421 tctttttgta gaaatggaat cagagaatta cgtacccggt ttcgggtgta tgcattgtct
     9481 ctatttgaaa gatcctcccg ccacgggatt ccgtgaaact cgagaatttc cttgagataa
     9541 ttcttcgaaa aggcgaggag aggtctgata aaggagtctc ttagttcagg gacgccgacc
     9601 aatcctcgga ctcctgttcc cctcatcaga ttcattaaaa acgtctccgc ggaatcatct
     9661 gaagtatgcg cgacggctac tccacaagcg cctgtttgct tcattgtctt ttcgaggaat
     9721 tcgtaacgaa tacgccgtgc gccgtcttca agcgattccc cccgttcaag gagttcaggt
     9781 acggggtacg aagcgctttc cacgggaaat gaaaaacggg tagcaatctc ctcgacgaag
     9841 cgctgatcct tgtccgcgct ttcacctctg ataccgtgat tgagatgcgc aacgatacaa
     9901 tgtccttccc acagggtatg gaaaaaccac atgagcgcca tggagtcgct cccgccggag
     9961 acggcgagca acatcgggcg gtcagtgcgc caccacccct ggcggatgcc gatatcatgg
    10021 aaaattgtgc gtaaacgctc ctgagaatga gaataaggga tggtttttaa ttgagaaacc
    10081 ttctcttttg aagtcacggc gacctcctga aaaggaaaaa aaggcggaga ttttctccgc
    10141 cttttacttt tgtcttttgt ggtggcggtg actggaatcg aaccagtgac actgcgggta
    10201 tgaaccgcat gctc
//
LOCUS       DAOSWW010000005         9953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_4301_length_9953_cov_4.301071, whole genome shotgun sequence.
ACCESSION   DAOSWW010000005 DAOSWW010000000
VERSION     DAOSWW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4301_length_9953_cov_4.301071"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /gene="nusA"
                     /locus_tag="PK442_00185"
     CDS             complement(<1..796)
                     /gene="nusA"
                     /locus_tag="PK442_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOO86097.1"
                     /translation="MQLGRDFARALAQITKERGLSQEVIASSLEAALISAYKKFQGGN
                     QDVEVFIDTENGDISICEVKQIVDVVTSPDVQISLEDAQSQGFVDVAEGDYIRIERDP
                     ENFGRIAAQTARQVIIQRLKDAERQIIFEEFSDRVGDLVTGVVFKSENDQVLIRLNDR
                     TEAILPREERIVSEKYIPGERMKLYLLDVRQTTRGPRIVVSRTHPGLLRKLLELEVPE
                     IREGVIDIKNIVREAGARAKIAVQTLDVNVDPVGACVGNNGARIKSI"
     gene            complement(798..1298)
                     /gene="rimP"
                     /locus_tag="PK442_00190"
     CDS             complement(798..1298)
                     /gene="rimP"
                     /locus_tag="PK442_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOO86098.1"
                     /translation="METGRKDGWKSLRAIVEGLGYECVGIALVTEERAVFLRVFIDSV
                     GGIQVRDCEIVSKAVSAFLDEHEEYIPGKYFLEVSSPGIERPLFTPEDYKRFIGKKAR
                     LRVRLPVQGRKSLSGLILSADDDAVELEIDADTPEEMTAVRISFDIITKGNLAFEEKK
                     DKRRNS"
     gene            complement(1432..1620)
                     /locus_tag="PK442_00195"
     CDS             complement(1432..1620)
                     /locus_tag="PK442_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86099.1"
                     /translation="MAGAKVFERNPYEDVVYWPGVIALPELMEDAMADDLLFADDPME
                     FETYLGEWWEGFVSDFYL"
     gene            complement(1659..3134)
                     /gene="guaB"
                     /locus_tag="PK442_00200"
     CDS             complement(1659..3134)
                     /gene="guaB"
                     /locus_tag="PK442_00200"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOO86100.1"
                     /translation="MSLESKFVPYEGFTFDDVLLEPAFSEVLPSDVSIKTNLTESIAL
                     NIPLCSSAMDTVTEARLAIAMAREGGIGIIHRNMPLEKQAKEVDVVKRSESGVIVDPF
                     FLHPEDMVREAISLMEHYHISGVPIVDAKKRLVGIITNRDLRFVQNFDQPISRIMTKD
                     NLVTASEGTTLEGAQSILMKYKIEKLPIVDENGILKGLITIKDIQKAKDFPNAAKDSG
                     GRLRVGAAVGVGADLFERAAALVKSGVDVLVVDTAHGHAKKVLDSVKNLRGAYPEMTI
                     IGGNIATAGAAEALIDAGADAVKVGVGPGSICTTRIIAGIGVPQLGAIYNVALAAHAR
                     GKTVIADGGIRYSGDIVKAIAAGADSVMIGSLFAGTEESPGEPIIYRGRSYKSYRGMG
                     SLGAMKEGCSKDRYFQESAVGDKLVPEGIEGLAAHKGSLSGVVFQLTGGLRSGMGYVG
                     ARDVQELQQKARFIKVTAASVKENHPHDVVVTKEAPNYWVD"
     gene            complement(3247..4413)
                     /gene="ispF"
                     /locus_tag="PK442_00205"
     CDS             complement(3247..4413)
                     /gene="ispF"
                     /locus_tag="PK442_00205"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOO86101.1"
                     /translation="MKEASLVIVAAGKGERAGGAGGTAKQFRPLAGIPLWKWSASAGE
                     RLFNKNVLHECILVVPESAVESVQRETLGWKIPFFVVPGGAERQESVVRGIRAASREY
                     VLVHDGARPFLSGALVERLLESLTDEYGVAPLLPVSDALKKTGEDGTFSPFPREGLWV
                     TQTPQAFHREKLLSVLQTHGGGAKDEAEAWITAGHPLRSVRGERKNIKITWEEDFSLA
                     ELYVQRTFRTGTGYDVHPLVPGRRFILGGVEFPEFPLGFAGYSDGDVLAHAVCDALLG
                     AAGLGDIGTLYPAGNPAFKNISSLLLLSDTAKRVTDAGWILEWSDSVICAQEPKLAAS
                     LSRMVSKMEEVLPETWRGRVHLKVKSGEHVGPVGHSHCIVCYSNATLWKPLDII"
     gene            complement(4410..5591)
                     /locus_tag="PK442_00210"
     CDS             complement(4410..5591)
                     /locus_tag="PK442_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOO86102.1"
                     /translation="MTEGFGKIVQLIFSGLMILIGGMIGAQVAQLLFPIIPAEWFAAW
                     KEWLPLWMPGKILMTIFSVVLFAFIGLMTSPLFLRVLGSMGSFIESHLKNTSWSEITS
                     ATVGMIIGLLIANLLALPFSDLPFGSYLAVVLNVVLAYLGARILLKRQSDLRNVFGPI
                     RSLKERLSMIKEHPHHDDEGFPAEEQPWEVPKKILDTSVIIDGRILDVARTGFLEGII
                     LIPQFVLHELQAVADSTDPARRTRGRRGLDVVNELQRLSDLQIEIVDASLKHLKADSV
                     DSALVVLAEKIGGQILTTDYNLNKLAQIQGIRVLNVNDLANALKPMLLPGETIIIDII
                     REGKEPHQGVGYLDDGTMFVVEDGENYIGKRVEVVVTSMLQTSAGRMVFGRIRREVRA
                     S"
     gene            complement(5669..6277)
                     /gene="recR"
                     /locus_tag="PK442_00215"
     CDS             complement(5669..6277)
                     /gene="recR"
                     /locus_tag="PK442_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOO86103.1"
                     /translation="MALPDSFERLASLFRRMPGVGAKSARRMAFFVLQQPPSFSDELA
                     RVLVALKERVHACSVCGNITDDDPCSICSDPLRDHRLLCVVETVEDLFSIEHAGVFAG
                     VYHVLGGRVSPLDGETLDERVLSALKEHIENDAIEEVIIATNPRIEGDLTFHALVEYL
                     GANNVALSRIAYGLPVGGSIEFADRTTLQAAMDSRVRVRRSS"
     gene            complement(6297..6602)
                     /locus_tag="PK442_00220"
     CDS             complement(6297..6602)
                     /locus_tag="PK442_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOO86104.1"
                     /translation="MNMEKIMKQAQKMQAQMAKIQEDLAGEQVEGSAGGGMVTVTCNG
                     QGDVVAVKIEPEVAGDDVEMLEDLVLAAVNDAIRKSKELANSRMSQLTGGLGMPGLF"
     gene            complement(6624..7085)
                     /locus_tag="PK442_00225"
     CDS             complement(6624..7085)
                     /locus_tag="PK442_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOO86105.1"
                     /translation="MKIIILSVGKPKDVHVVSLAEHYFKRLRSFVPVVLESVPEAKDP
                     LPSKKVEREGVGLLKNLRERDFLVTLEERGKCFDSREFAEWVQTSVERTPGRVVLAIG
                     GAFGLSEDVRRRSDFSLSLSPMTMPHELCLVFLMEQLYRAFTLIRGVEYHH"
     gene            complement(7231..8877)
                     /locus_tag="PK442_00230"
     CDS             complement(7231..8877)
                     /locus_tag="PK442_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOO86106.1"
                     /translation="MDKTQYNYFRDLTLIAAWILAALCVGGPSSRFIVGMAILAAVFG
                     MVQHMYPNSLWKPVYLLIGFIFALFGPRIAFIGLPAGQYHYLPVEVSVLITALWIAAF
                     PVLLQELDNIPGMAGHLLAVSFSLLLTATVFSGQSLQDAFFMSLSGLLFLGVFWSRHG
                     HMYRRMGEALAAMWGVLVAGTSVLGVSKGITFGTLMVLPLGLFAIPIMEASLNFMNRA
                     FSSKSPNAAVLYSKLIGKGLDHPSAVRFITSLCALGGSAVAISQLGHPLSMMFVVSLA
                     ILFAGITLAPAIRDMKENVEMHQRPSIWRTTIDNVSMNYALAKARGALASRSGARLVA
                     TVNALAVMEAEKNDKYANALDGSFLTLADGAGLVWALKLLGTPVQERVTGIDYMTGLA
                     RMAAAEGWPVYLLGSKPSIVEKAAFELQRRFPDLTVAGFRDGYFDEGDVSVIDEIRES
                     GAKILFVAMGIPKQEIWVTENAERLGDLLAVGVGGAFDVVSGTLKRAPELLQKIGLEW
                     FYRLLQEPWRWKRDLDLFVFVIKVLLTKTGLYRSRARRTV"
     gene            complement(9054..>9953)
                     /gene="serS"
                     /locus_tag="PK442_00235"
     CDS             complement(9054..>9953)
                     /gene="serS"
                     /locus_tag="PK442_00235"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOO86107.1"
                     /translation="RWGEPKSFAFEPKAHWDIGEAAGIMDFEKGAALAQSRFTVLKGV
                     GARLERALLNFMLDLHTEKHGYLEVQPPFMVNSKTMQGTGQLPKFADDLYKCENDDLW
                     LIPTAEVPLTNLFAGEILEEEQLPFYCTAYTPCFRREAGAYGRDVRGMLRQHQFDKVE
                     MVKISTPERSYEELEMLTDNAEEVLRLLEIPHRTVCLCTGDMGFGSSKTYDIEVWLPS
                     QDKYREISSCSNCEDFQARRMNTRYRPKGGGKPQFVHTLNGSGIAVGRTLIAVLENYQ
                     REDGSVEIPKALVSYMGGVEEIR"
BASE COUNT         2407 a         2953 c         2436 g         2157 t
ORIGIN      
        1 tgatcgactt gatcctcgcc ccgttatttc cgacgcatgc gccaacggga tccacattga
       61 catcaagcgt ctggacggcg atctttgccc gggctccggc ctcccggaca atatttttta
      121 tatcgatgac tccctcgcga atctccggaa cctccagctc aagaagtttt ctgagaagac
      181 ccggatgcgt ccttgaaacg acgatacgag gacctcgcgt cgtctgcctg acatcgagca
      241 gatagagctt catacgctcc cccgggatat atttttcact cacgattctc tcctcacgtg
      301 gaagaatcgc ctcggtccta tcgttcagac ggatgagcac ctggtcgttc tcggatttga
      361 aaaccactcc ggtcacgaga tcccccaccc tatccgaaaa ctcctcgaaa attatctgcc
      421 gttccgcatc cttgagcctc tggataatca cctgacgggc tgtctgggcg gcaattcggc
      481 cgaagttctc cggatcgcgc tctatcctga tgtaatctcc ctcggctaca tcgacaaacc
      541 cctgagactg tgcatcctcc agggatatct ggacatccgg actcgtcacg acatccacaa
      601 tctgcttcac ctcgcagatg gagatatcgc cgttctccgt atcgataaaa acttcgacgt
      661 cctgattccc tccctggaat ttcttatacg ccgaaatcaa cgccgcctca aggctcgacg
      721 caataacttc ctgcgagaga cctcgttcct tagtgatctg agccagagca cgggcgaagt
      781 cacgaccaag ctgcatttta agaattcctc cttttatctt tcttctcctc gaaggcaaga
      841 ttccctttag ttataatatc aaatgaaata cggacagctg tcatctcctc gggagtatcc
      901 gcatcgattt cgagctcgac cgcatcatca tcggcggaga ggatgagtcc gctgagactc
      961 ttccttcctt gaacgggtag gcgcaccctc agtcgcgcct tctttccgat aaaacgttta
     1021 tagtcctccg gcgtaaacaa gggacgctca atccccggag aactcacttc caggaaatac
     1081 ttgcccggaa tgtattcttc atgttcatca aggaacgcgc tcacggcctt agagacaatt
     1141 tcgcaatccc ggacctgaat gcctcccacg gaatcaataa aaacacgcag gaaaacagcc
     1201 ctttcttcgg tgacgagagc aatcccgaca cattcgtatc cgaggccttc cacgattgca
     1261 cgcaacgatt tccatccatc ttttctgcct gtttccatat tatcgccccg attcactctt
     1321 cgtaaaaaac aaagagtggg ggaaatccca ctctttgcaa aaaaatcccg caaaaaaatc
     1381 attatagagt atagcacaaa aaaagtcttt gcgcagcgtc actccacaat tttacagata
     1441 aaaatcggaa acaaacccct cccaccactc cccaagatac gtctcgaact ccatggggtc
     1501 gtcggcaaag agaaggtcat cagccatagc atcctccatt aattcgggaa gagcaattac
     1561 cccgggccag tagacgacat cctcgtacgg attccgttca aaaacctttg ctcccgccat
     1621 atcgcacccc cgttctcggg aagagcaggc ctaccccatc aatccaccca ataattcggc
     1681 gcttccttcg tcaccacgac gtcgtgaggg tgattctcct tcacagaggc cgccgttacc
     1741 ttgataaaac gcgctttttg ctgaagttcc tgaacatccc gcgcaccgac ataccccata
     1801 ccggagcgca agcctccagt cagctggaag acaacgcctg agagagaacc tttgtgcgcc
     1861 gctaacccct caataccctc cggaacaagt ttatcaccaa ccgcgctttc ctggaagtac
     1921 cggtccttgc tgcacccttc tttcatcgcg ccgagcgacc ccatgccgcg gtaactctta
     1981 taggagcgcc ccctgtagat gatgggttct cccggactct cctcggtgcc cgcaaaaaga
     2041 gaaccgatca tgacggaatc cgcgcccgcc gcgatggctt tcacaatatc tccggagtag
     2101 cggattccgc cgtcggcaat gacggtcttt ccgcgagcgt gcgctgccag agcgacattg
     2161 taaattgctc ccagctgcgg gacgccgata ccggcgatga ttcgcgttgt gcagatgctc
     2221 ccgggaccga ccccgacctt gacagcatcc gctccggcgt cgatgagggc ctcggcggct
     2281 ccggcggtgg cgatgtttcc tccgatgatc gtcatctccg ggtacgctcc ccggaggttc
     2341 ttcacggaat cgagcacctt ttttgcatgc ccgtgcgcag tatcgacaac gagcacgtcg
     2401 acgccgctct tcacgagagc ggcggcccgt tcaaaaagat ccgccccgac tccgacagcc
     2461 gcgccgactc gaagacgtcc tccgctgtcc ttcgcagcat tcgggaaatc cttcgccttc
     2521 tggatatcct ttatcgtgat aaggcctttc agaatgccgt tctcatccac aatcggcaac
     2581 ttctctatct tgtacttcat cagaatggac tgcgctccct ccagcgttgt tccttccgac
     2641 gcagtcacaa gattgtcctt cgtcataatc cggctgatag gctggtcgaa attctggaca
     2701 aaacgcagat ccctgttggt gatgataccc acgagccgtt tttttgcatc cactattgga
     2761 acgccggaaa tgtgatagtg ttccataaga gagattgctt ccctcaccat gtcctcgggg
     2821 tgaagaaaaa acggatcgac gatgacgccg gattcagaac gcttgacaac gtccacttcc
     2881 ttggcctgtt tctccagcgg catgttcctg tggataatgc ctatcccgcc ctctcgggcc
     2941 attgcaatgg ctaaacgagc ctccgtcaca gtatccatcg cggaactgca cagagggata
     3001 ttcagagcaa tcgattcggt cagatttgtc ttgatagaaa catcggacgg caaaacctca
     3061 ctgaacgccg gttcgagaag aacgtcatca aatgtgaatc cctcgtaggg gacgaacttg
     3121 gactctaaac tcattacggt acctccctgc ccgttaggga ctcgtaaaag attttaaagg
     3181 attgtagggc tttccggaaa aagaagcaac tccccttacg aaaaaaacaa caaaaaaacg
     3241 tccggctcaa ataatgtcaa gaggtttcca gagcgtcgcg tttgagtaac agacgatgca
     3301 gtgagaatgt ccgaccggtc ctacgtgttc cccggatttc actttgaggt gcactctgcc
     3361 ccgccacgtt tcagggagaa cttcttccat cttcgagacc attcgggaga gagaagcggc
     3421 gagcttcggc tcctgagcgc aaatgacact gtcgctccac tcaaggatcc atcctgcgtc
     3481 cgtgactctt ttcgcagtat ccgaaagcag aaggagactc gagatgtttt tgaacgcggg
     3541 attccccgcc ggatagagcg tgccgatatc acccaaccct gcggccccca gaagcgcgtc
     3601 gcacacggca tgcgccagaa cgtccccgtc cgagtaccca gcgaaaccga gaggaaactc
     3661 cgggaattcg acaccgccga gaatgaacct tctgccggga acgagagggt gcacatcata
     3721 cccggttccg gtacggaaag ttcgctgcac atagagttcc gccagagaga agtcctcctc
     3781 ccatgttatc tttatatttt tgcgctctcc cctgaccgaa cggagaggat ggcctgcggt
     3841 gatccatgcc tccgcctcgt ccttggctcc gcctccatga gtttggagca cggagagcaa
     3901 tttctcccgg tgaaaggcct gcggagtctg cgtaacccag agcccctctc tcggaaaggg
     3961 cgaaaaagtc ccgtcctctc ccgtcttttt cagggcatcg gatactggaa gcaggggcgc
     4021 cactccgtac tcgtccgtaa gagactcaag gagcctttcc acaagagcgc cggagagaaa
     4081 aggccgcgcg ccgtcgtgga cgagcacgta ttcccgggaa gcggcgcgga taccgcgcac
     4141 gacggattcc tgccgttcgg ctcctcccgg cacaacgaaa aacgggattt tccacccgag
     4201 cgtctccctc tgcaccgact ctacggcgct ctccggaacg acgagaatac attcgtggag
     4261 tacgtttttg ttgaagaggc gttctccggc ggaagcgctc cacttccata ggggaatacc
     4321 ggcgagcgga cggaactgtt tcgctgttcc gcccgctcct ccggcacgct ctcccttgcc
     4381 cgccgcaacg atgacaagag aagcttcttt cacgacgcgc gaacttccct tcgtatccgt
     4441 ccaaacacca tccggccggc ggatgtctgg agcatggagg tgacgacaac ctcaacccgt
     4501 ttgccgatat agttctctcc atcctcgacg acgaacatcg tcccgtcgtc gaggtagccg
     4561 actccctgat gaggttcctt tccctctcgt ataatatcaa ttatgatggt ctctccggga
     4621 agcagcatcg gtttgagcgc atttgccaga tcgttgacgt tcaacacacg aatcccctgg
     4681 atctgcgcga gtttattgag gttgtaatcg gttgtaagaa tctgtccgcc tatcttctcg
     4741 gctaaaacga ccagcgcgct gtcaacggaa tccgctttca gatgcttcaa tgaagcgtcg
     4801 acgatttcaa tctgcagatc ggaaaggcgc tgcaactcgt tgacgacgtc aagccctctt
     4861 cgccccctcg ttcttcgcgc ggggtcggtg gaatcagcca cggcctgcaa ttcgtgaaga
     4921 acaaactggg gtataagtat aatcccctcc aagaagccgg tgcgtgcgac atcaagaata
     4981 cgcccgtcga tgataacgct cgtatccaga atttttttgg gaacctccca gggctgttct
     5041 tccgcgggaa aaccttcatc atcatggtga gggtgctcct tgatcatgga aagacgttct
     5101 ttcaggcttc gaattggacc gaatacgttc cggagatcgc tttgccgctt cagtagtatt
     5161 cgagccccaa gataggccaa aacgacgttc aacacaacag ccaaataact accgaatggg
     5221 agatcggaga atggaagagc cagaagattc gcgatgagca acccgatgat cataccgacg
     5281 gtagctgaag taatttccga ccagctcgta tttttgagat gcgattcgat gaaggagccc
     5341 atagaaccaa gaacccggag gaaaagggga gaggtcatca aaccaataaa agcgaataaa
     5401 accacagaaa aaattgtcat cagtatcttg ccgggcatcc aaagggggag ccactctttc
     5461 caggcggcaa accattcggc gggaataatg gggaaaagta actgagctac ctgggcgccg
     5521 atcatgccgc caataagtat cataagccct gagaaaatca actggacgat tttcccgaaa
     5581 ccttccgtca tactttcacc tcgctttttt gacaaatatg tcatcagctt aaactttttc
     5641 acagcacgtc tccttttcca agacacaatt aggaagatcg cctgactcgt acgcgggaat
     5701 ccatcgccgc ctgcagcgtc gtccgatcag cgaactcgat actgcctccg acaggaagcc
     5761 cgtacgcaat ccttgaaaga gcgacgttgt tggcgccgag gtattcaact agagcgtgga
     5821 atgtcagatc gccttcaata cgcggattag tggcaatgat cacctcctcg atagcatcat
     5881 tttcaatgtg ctctttcaag gcggacagga cgcgctcgtc gagcgtctcg ccgtccaggg
     5941 gagagactcg tcctccaaga acatggtata ccccggcaaa aacacccgcg tgttcgatgc
     6001 tgaaaaggtc ctcgacggtt tccaccacac agagcaaacg gtggtcccga agagggtcgg
     6061 agcagatcga acagggatca tcgtccgtta tgttgccgca aacggaacag gcatgaacgc
     6121 gctccttcag cgcgacaagg acgcgggcga gctcgtcgga aaaagaaggc ggctgctgca
     6181 ggacgaaaaa agccatacgc cgggcgcttt ttgctccgac acccggcatt ctccgaaaca
     6241 atgatgcaag tctttcaaag gaatcaggaa gagccacgga gaagcccggg aacgccctag
     6301 aaaagaccgg gcataccaag gccgcccgtc agctgactca tcctgctgtt cgcaagttcc
     6361 ttgctcttac ggatcgcgtc gttcacagcg gcaagtacaa gatcctcaag catctcaaca
     6421 tcgtcccctg caacctcggg ctcgatcttg actgcaacga catccccctg tccgttgcag
     6481 gtcactgtga ccataccgcc ccccgcgctc ccctcaacct gctcgcctgc gaggtcctcc
     6541 tgaattttcg ccatctgggc ctgcatcttc tgcgcctgtt tcataatttt ttccatattc
     6601 atatcaaaag cacaactcct tttttaatga tgatactcca cgccgcgaat cagagtaaac
     6661 gcacgataaa gctgctccat gagaaaaacg aggcacagct cgtgcggcat cgtcatcggc
     6721 gaaagagaaa gtgaaaagtc gctccgacgc cgaacatcct ccgaaaggcc gaacgcgcct
     6781 cctatcgcca gaacaactct tcccggcgtt cgctcaacac tagtttgcac ccattcggca
     6841 aactcacggc tgtcaaagca ttttccccgc tcttcaagcg ttaccaggaa atctctttcg
     6901 cgaagattct ttaaaaggcc gactccctcg cgctccactt tcttcgaagg aagcgggtcc
     6961 tttgcttcgg gcacgctctc aagcacaaca ggaacaaaag aacgaagacg tttaaagtag
     7021 tgttccgcga gcgaaacaac atgaacatct ttcggttttc cgacactaag gataatgatt
     7081 ttcatagtat atgtccagca cgtttctcgt attttctctt tgtcgatcga tcctgtgaac
     7141 cggacaacaa aaaacgcgcc cctccgtctt cgcagaaaac atcagggcgc acataataca
     7201 gagggagcca atttttcaga aggagtttca ttacaccgtt cgtcttgctc gtgaacggta
     7261 aagacctgtt ttcgtcagaa ggacttttat cacaaacaca aaaaggtcaa gatccctttt
     7321 ccatctccaa ggctcctgaa gaagacggta gaaccattca agcccgattt tctgaagaag
     7381 ctccggagct cgtttcagag tgcccgacac gacgtcgaaa gcaccgccga cgcctacggc
     7441 aagaagatcg cccagccgtt cggcgttttc ggttacccat atttcctgct tgggaatacc
     7501 catagcaaca aaaaggattt ttgcgccgct ctctcgtatc tcatcaatca cggaaacgtc
     7561 gccctcgtca aaatatccgt cgcggaaacc cgcaacagtg agatcgggaa accggcgctg
     7621 caactcgaac gccgctttct ccacgatcga gggcttcgat ccgagaagat agaccggcca
     7681 cccttcggca gcagccatac gagcgagtcc ggtcatgtag tctattccgg tcactctctc
     7741 ctgcactggc gtaccgagca gcttgagcgc ccatacgaga ccggcgccgt cggcaagggt
     7801 caagaacgaa ccgtctaaag cgttcgcata cttgtcgttc ttctcggcct ccatcacggc
     7861 aagtgcgttt accgtcgcga caaggcgtgc gccgctcctt gaggcgaggg ctccgcgggc
     7921 ttttgcaagc gcatagttca tagaaacgtt gtcaatagta gtccgccaaa ttgaaggtct
     7981 ctggtgcatt tccacattct ccttcatatc gcgtatggcc ggagccaaag taataccggc
     8041 gaaaagaata gcgaggctca caacaaacat catggagaga ggatgtccaa gctgggagat
     8101 ggcgacggcc gaccctccga gagcgcagag cgacgtgatg aagcggacgg cgctgggatg
     8161 atcaagtccc tttcctatga gtttgctgta cagtacggcc gcattcggcg acttcgagga
     8221 aaaggcacgg ttcatgaaat tcagcgacgc ctccatgatg ggaatcgcaa aaagacctag
     8281 agggagcacc atcagagtac cgaaggtaat acctttgctc acgccgagaa cggaagtgcc
     8341 ggcgaccaaa acgccccaca tcgcggcgag cgcctctccc attcttcgat acatgtgccc
     8401 gtgacggctc cagaaaactc cgaggaagag caagcccgac agggacataa aaaaagcatc
     8461 ctgcaaactc tgaccggaaa agaccgtggc ggtcagcaga agggagaacg aaacagcaag
     8521 cagatgcccg gccatgcccg gaatgttatc cagttcctga agcagcacgg ggaaggcggc
     8581 aatccagagg gcggttatca gtacggagac ctcaaccggc aaatagtgat actgcccggc
     8641 gggcaaacca ataaaggcga tgcgcggacc gaaaagagca aaaataaaac cgataagcag
     8701 gtagaccggt ttccagagcg agttcggata catatgctgc accatgccga ataccgccgc
     8761 aaggatcgcc attccgacga tgaaacggga agacggcccc ccgacgcata gtgcggcaag
     8821 aatccaggcc gcaataaggg tcagatcacg gaaatagttg tattgcgtct tgtccatgct
     8881 ccttctgcag gcgcgctgcg caaaaaaaca gagcaccgta agaggaagca cacataaaag
     8941 aatgtcggta acgtacattt ccgcgtggaa aatatccagt tccctcccct gcaaaaaagt
     9001 ctttgatgat tgtatcagct gtcgctgtat ttttccaccg ttctttgccg aggttaccgg
     9061 atttcctcca cgccgcccat ataggaaaca agcgccttcg gaatctccac ggaaccgtcc
     9121 tcgcgctgat agttctcgag aacggcgatg agcgtccgtc ccaccgcgat gcccgagccg
     9181 ttgagcgtgt ggacgaactg cggcttgccc ccgcccttcg gacggtagcg cgtattcatt
     9241 cttctggcct ggaagtcctc gcagttactg cacgaactga tctcccggta cttatcctgg
     9301 gagggcagcc acacttcgat gtcgtatgtc ttgctggagc cgaaccccat gtcgcccgtg
     9361 cagagacaga cagtccggtg cggaatttcg agcaggcgga gcacctcttc cgcattgtcc
     9421 gtcaacatct caagctcctc gtagcttctc tccggcgtgc tgatcttgac catctcgacc
     9481 ttgtcgaact ggtgctgccg aagcattccg cgcacatcac gcccgtacgc tcctgcctct
     9541 ctgcggaaac atggcgtata ggcagtgcag tagaacggaa gctgctcctc ctccaggatt
     9601 tcgcctgcga agaggttggt gagggggacc tccgcggtcg ggatcagcca cagatcgtcg
     9661 ttctcgcact tgtagagatc gtcggcgaac ttcgggagct gccccgttcc ctgcatcgtt
     9721 ttgctgttca ccatgaaggg cggctgcacc tcaaggtagc cgtgcttttc cgtgtgaagg
     9781 tccagcatga agttgaggag cgcgcgttca agacgggctc ccacaccttt cagtacggta
     9841 aatctgctct gggccagcgc cgcgcccttt tcgaagtcca tgatacccgc cgcctcgccg
     9901 atatcccagt gcgctttcgg ttcgaaagca aaagatttgg gctctcccca gcg
//
LOCUS       DAOSWW010000006         9401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_4654_length_9401_cov_4.282153, whole genome shotgun sequence.
ACCESSION   DAOSWW010000006 DAOSWW010000000
VERSION     DAOSWW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4654_length_9401_cov_4.282153"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1191
                     /gene="upp"
                     /locus_tag="PK442_00240"
     CDS             91..1191
                     /gene="upp"
                     /locus_tag="PK442_00240"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOO86108.1"
                     /translation="MKIAVGSDHAAFALKNYLLEHLRQKSGIESVDMGVDTDAVSCDY
                     PDVAFRVGEAVVSRQADRGLLLCGTGVGMSIAANKIPGVYAAHCHDVETARFSRRHNN
                     ANVLTMGARIISQELAREIIDVWLETDFEGDRHIRRLSKILDYEHKICPSCTFSEKGK
                     TVVFEHPLVQHKVSIIRDKNTSVKEFRELVQEIAGLMVYEITRNLPLVKIEVETPLAK
                     TEAYALEGKKLAIVPVLRAGLGMVDGILQLVPNAKVGHIGLYRDPETLLPVEYYCKLP
                     GDISERDILVLDPMLATGGSAAAAISLVKARGGKKVSLVCIIAAPEGVSKVHAEHPDV
                     DIYAAALDSHLNDHGYIVPGLGDAGDRLFGTK"
     gene            1201..2094
                     /locus_tag="PK442_00245"
     CDS             1201..2094
                     /locus_tag="PK442_00245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOO86109.1"
                     /translation="MDSLVAPLSVSIPLLFLWGVCVTPLSILLSNRYRIVDQPGERKI
                     HTSVTPRGAGIVLWLGFLLWSLYAVEEAPLLRFMAVGSTLVFLSGYWDDMKTLNPAIR
                     LTFHIVAAGLFLLFLPVSLVHIPVCLLWITGMTNAFNLIDGANGLCLMMFIVASSVFA
                     ACGFPFFFLPVASLAAGVLLWNFPRAKTFLGDGGTTLLGYLYSSLCLYSLTPFLEDVH
                     VFELPFFLLMAGGIPAADTLFAIMRRLAMRKSPFSPDRGHIHHRLLDRGWSPVLTVMI
                     LSVAQLLVVAGGVAFFGRFLV"
     gene            2148..3284
                     /gene="wecB"
                     /locus_tag="PK442_00250"
     CDS             2148..3284
                     /gene="wecB"
                     /locus_tag="PK442_00250"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOO86110.1"
                     /translation="MEKKIVCVVGTRPEGIKMAPLIRVLRERNFPVTVLSTGQHLQML
                     HQSLEFFGIEADVNLSVMEERQTLDRVTAKVLGGVGAFLDRRPQDLVLVHGDTTTTMA
                     SALAAFYRSIPVGHVEAGLRSGDMARPFPEEANRIITDRLSSLWFAPTEQAAANLKNE
                     GLPVSEGTLAITGNTVIDALKLVLERKHDLCDSLRPLERFPGPLLLMTAHRRESWGEP
                     LESICGAIVDILKQNSEVRILIPLHKNPSVRDVIRRYLADEERAILCEPLDYPDFVWA
                     MQRSSVILSDSGGVQEESAELKKPLLVMRDVSERPEALETGTALLVGTNRVGIAETAL
                     RILSDDVFRKSLLSRGANPFGSGDASEKIADVIAKFFENRQGVA"
     gene            3328..4626
                     /gene="murA"
                     /locus_tag="PK442_00255"
     CDS             3328..4626
                     /gene="murA"
                     /locus_tag="PK442_00255"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOO86111.1"
                     /translation="MESKMKIIGGTQLKGNITAQGAKNAALPVMAAAILLKGKKLKID
                     RVPNLLDVVTMADLLRSLGAVVQFSDHEMEISTPEEISWETPPDLVRKMRASSLVLGP
                     LLARCGKAVMPLPGGCSIGSRPIDLHLKGLTRMGASIELVHGAVHARADGLRGCRIYL
                     DFPSVGATENLMMAAVFAQGETVLENTAREPEIFNLVEALRSMGAFIDADGTGVIRIK
                     GVDDLESASATVIPDRVEAATYILAGAATNGEVTVSDIIPNHIDSLLAKLEEAGALMT
                     IKETEVTIHPSGRLKAVSLKTLPYPGFPTDLQPQVMVALCLAEGTSVVQESVFQSRFL
                     HVSELNKMGAKIDIQSNSAIINGVASLAGADVLATDLRAGAALVLAGLAARDITYVHH
                     LGHISRGYELMEEKLRAIGANISILPSVEDSKGDSPALRS"
     gene            4751..5503
                     /locus_tag="PK442_00260"
     CDS             4751..5503
                     /locus_tag="PK442_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86112.1"
                     /translation="MVAVENEVDYIIDDGLVIAKGRIMVGKSAKAEKNLVRAIRRALF
                     QFPEHRKAVLAFFQKKQPKKLMIIATSISMAEKITRALRLPYPEMIIDITEVASAEEI
                     INARRERHEKGQHVIPASRTQIRKNFAGKLVGHLRGLFKSVDKEDGERTIVRPPFSFY
                     GELTIESSAILDLVRHVLSSNPQITEVKDIRVRYKDDAISMEIVLQVIPGKMDFHRLG
                     VVNQKRVASAVSYFTGMDVDGVDVHIVEVMLP"
     gene            5500..6117
                     /locus_tag="PK442_00265"
     CDS             5500..6117
                     /locus_tag="PK442_00265"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOO86113.1"
                     /translation="MSVFASGADSGLAWEELKRRVETCSSCGLCSTRKKTVFGQGSET
                     SPVVFVGEGPGADEDEQGVPFVGRAGKLLTRILQSVGIERESVFITNVVKCRPPQNRT
                     PMADEMMACTPFLEAQMALLRPKIVVCLGNTPAKWLLKSSEGISALRGRWFAWRGIDL
                     FPMFHPSYLLRNESKKKGSPKDLTWQDINALRSKLDDLTKGGPSA"
     gene            6114..6812
                     /locus_tag="PK442_00270"
     CDS             6114..6812
                     /locus_tag="PK442_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOO86114.1"
                     /translation="MIPKETVPRHVAIIMDGNGRWAKKRFLPRLLGHKAGVKAIERTV
                     RAADALGIQYVSLYAFSTENWKRPEKEVSGLMSIFRFVLRRKVDEMKRENVRLRFSGF
                     RDGMPQDIVELMEWAEEETKGNSGLTLVPCLNYGGRQEILHAVEKLLEDGARPPLTEE
                     RFREYLYLPDLPDPDLIVRTSGESRLSNFWLWQSTYSELYFTDTLWPDFDGKELENAV
                     KYFANRERRYGALK"
     gene            6796..7620
                     /locus_tag="PK442_00275"
     CDS             6796..7620
                     /locus_tag="PK442_00275"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOO86115.1"
                     /translation="MALSSEKLRELTLRGGSSLVIVAGTLSSLYVGGMVWTFFASAVA
                     LISLAEFYGMLSRKFKVSKGIGFLTSGLIIFSVSEGIHPVSLVLTLSVSAFVVLFVEI
                     IRRQLRGESFAIWNMGGTISGIVYITIPWTFMILLRRLPGGGLLLLAMFIATWSCDVA
                     AYLIGSRWGRTLLCVNVSPNKTWEGFFGGFSAALLASVLLAFILEIPPIPFVYVGIIC
                     GFAGQLGDLAESVIKRESWVKDSGTLIPGHGGVLDRFDSTLINGLLVYVLFGVILR"
     gene            7617..8795
                     /gene="dxr"
                     /locus_tag="PK442_00280"
     CDS             7617..8795
                     /gene="dxr"
                     /locus_tag="PK442_00280"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOO86116.1"
                     /translation="MKKRLFVIGCTGSVGSSVLSVCRMFPEHFEIAALAACSSVDRML
                     LLAEEFSCPTLVFSDESAAVRAANELLSSGVQYAGRRVLAGEQGLLEMASHPDIDHVV
                     FASSGTGAIGALMAALSSGKEVSLANKESIVVAGPWVLPLVLRPDQIRPLDSEHNAIW
                     QCLSGEDRRFVNKIFLTASGGPFRSFSQEEMKRVTPSMAVAHPVWNMGAKISVDSATL
                     MNKGIEILEAMYLFSFSADQVDAVISPNPFIHGLVCFSDGTFHMCASAPDMGIPALSA
                     LFSPLRSPRDRKNTPVLDGMQISFEAPDEVRFPCIRLAREVARKEGAYPALLVGADEV
                     AVDRFLSGRVSFMSIPRIVESVLEEYSGPSPSTLEHALHILEEGRRMAEKVSSRLEEK
                     "
     gene            8816..>9401
                     /locus_tag="PK442_00285"
     CDS             8816..>9401
                     /locus_tag="PK442_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO86117.1"
                     /translation="MVSLVSFLLVIGICVIIHELGHYFTARIFDVQVHEFAFGMGPVL
                     LQRQGRHTMWSFRAFPVGGFVRLAGMGEDHEEEVVEEGRGFFDKAAWKRFLILANGSV
                     SNILLAMLLTVFFLSGHGVINLESTEIGEIMEGYPAQTAGILPGDAIVAVNDVPVSSW
                     REMSESIRTTASEGPVLFSVLRKGETLSLTVSIPP"
BASE COUNT         2081 a         2225 c         2666 g         2429 t
ORIGIN      
        1 caaatgatat tatataaggg ttgaagagtg ttaccggggg ctcgacgttt ctttctttct
       61 cctggaaatt gtacgaggag gggtctgtcc atgaagattg ccgtgggttc tgatcatgct
      121 gcgtttgctt tgaaaaacta tttgcttgag catcttcggc aaaaaagtgg catcgaaagc
      181 gtggatatgg gtgtggatac ggatgcggtt tcttgtgatt atccggatgt ggcgttccgt
      241 gttggagagg ccgttgtttc ccgccaggcg gacagaggcc ttcttttgtg cggtaccggt
      301 gtcggaatga gtattgcagc gaacaagatc cctggagtat acgcggccca ttgtcatgac
      361 gtggaaactg cccgattcag ccgccggcat aataatgcga atgtcctcac catgggggcg
      421 cggataatat ctcaggaact tgcccgcgaa ataatcgatg tctggctcga aacagatttt
      481 gaaggagacc ggcatataag aagattgagt aaaatccttg attatgaaca caaaatctgt
      541 ccaagctgta ccttttccga aaaaggcaag acggtcgtct ttgaacaccc gcttgtgcag
      601 cacaaggtaa gcatcatccg cgacaaaaat acctcggtga aagaattccg tgagcttgtg
      661 caggagattg cagggctgat ggtctatgag attacccgaa atctgccgct cgtaaaaata
      721 gaggtggaaa cccctctcgc gaagacggaa gcctacgccc ttgaggggaa aaaactcgcg
      781 atagtacccg tcctcagagc gggcctcggc atggtcgacg gcattctcca actcgttccg
      841 aacgcaaaag tcgggcatat cgggctctat cgtgatcccg agacgcttct ccctgtggag
      901 tactactgca aactgcccgg cgatatcagc gagagagata ttctcgttct cgatccgatg
      961 ctcgccacgg gaggttcggc tgccgcagcc atcagtctcg tcaaagcacg gggcggcaaa
     1021 aaggtttcac tcgtttgcat catagcggct cctgaaggcg tctccaaagt gcacgccgag
     1081 catccagacg ttgatatata cgcggcggct cttgacagtc atctcaatga ccacggatat
     1141 atcgttcccg ggcttggcga cgccggagat cgtctcttcg ggacgaagta agggactttc
     1201 gtggattctc tcgttgcgcc tctgtcggtt tcgataccgc ttcttttcct ctggggagtg
     1261 tgcgttacac ctctttcgat cctcctctcc aatcggtacc gtatagtcga ccagcccggg
     1321 gagcgtaaaa ttcatacttc agtcactccc cgaggagccg gaattgttct ctggctagga
     1381 tttctcctat ggagcctcta tgcagtggag gaggctcctt tgcttcgttt catggcggta
     1441 ggttctaccc tcgtgttttt aagcggttac tgggacgata tgaaaacttt gaacccggcc
     1501 atacgactca cgtttcatat tgttgccgca ggtcttttcc tgctttttct ccctgtttct
     1561 ctggttcata tacctgtgtg cctgctctgg attacaggaa tgacgaacgc tttcaacctt
     1621 atcgacggag cgaacggcct gtgcctcatg atgtttatcg tggcatcctc ggtttttgca
     1681 gcgtgcgggt tccccttttt tttccttcca gtcgcgagct tggctgctgg agttcttctc
     1741 tggaattttc ccagagcgaa aaccttttta ggcgacggcg gaacaacgct tctggggtat
     1801 ctctactcaa gcctttgtct ttattccttg accccgtttc tcgaagatgt tcatgtgttt
     1861 gaactgccct tttttctgtt gatggcaggc ggtattcctg cggcagatac gctctttgct
     1921 ataatgagac gattagcgat gagaaaatct ccgttttcac ctgatcgcgg acacattcat
     1981 catcgtttgc tcgacagagg atggagcccc gtccttactg taatgattct tagtgttgca
     2041 cagttgttag tagtcgccgg cggggtcgcg tttttcggcc gttttctcgt gtaacagtgt
     2101 ccggagagtg tgcttttaac aggatttttg cgtagggatg gctgaggatg gaaaaaaaaa
     2161 tcgtatgtgt agtcgggacc cgtcccgaag ggataaaaat ggcgcctctg atccgtgtgc
     2221 ttcgggagcg gaacttcccg gtgacagtgc tttcgacggg gcagcatctt caaatgctgc
     2281 atcagtcatt ggagtttttt ggaattgagg ccgatgttaa tctttccgtg atggaggaac
     2341 gtcagacgct cgacagggtg accgccaaag tgcttggtgg ggtaggcgct tttcttgatc
     2401 gtcgaccaca agacctggtt ttagtacatg gcgataccac gacaaccatg gcctctgcgc
     2461 tcgcggcctt ttaccggagc attccggtag gccatgtcga ggccggcttg cgaagcggcg
     2521 atatggcccg tcctttcccg gaggaagcga acaggattat cacagacaga ctgtcttcat
     2581 tatggttcgc tccaacggag caagctgcag cgaaccttaa gaatgaaggg cttcctgtat
     2641 ctgaagggac tcttgcaatc acggggaata cggttattga tgcgctgaag ctggtattgg
     2701 aaagaaaaca tgacctctgc gattctctac gtccgctaga gcgtttcccg ggacctctcc
     2761 ttctcatgac cgcgcatcgc agagagtcat ggggggagcc tcttgaatca atatgtggag
     2821 ctattgtgga tatactgaaa caaaacagtg aggtgcggat acttataccg cttcataaaa
     2881 atccttcagt acgtgatgtt atcaggcggt atctagcgga tgaagagcgg gctattcttt
     2941 gcgaaccact cgactaccct gattttgtct gggcgatgca aagaagctcc gttattttga
     3001 gcgacagcgg aggcgtccag gaggaatcag cggaattgaa aaaacccctc ctcgtgatga
     3061 gggatgtttc ggaacgaccg gaagctctcg aaacaggtac ggctcttctc gtcggaacca
     3121 atcgggtcgg gatagcggag acggcgttac gcattctctc cgacgatgtt ttcaggaaga
     3181 gtcttttgag ccgcggggcg aacccttttg gaagcggcga cgcttctgag aaaatagctg
     3241 atgtaatagc gaagttcttt gaaaacagac agggtgttgc ctgagtcttt ttgtgttttt
     3301 cacaatattt tcgggaggaa ttggaaagtg gaatccaaaa tgaaaatcat cggcggtacc
     3361 cagctgaagg ggaatataac cgcacagggc gctaagaatg ccgcgcttcc tgtgatggcg
     3421 gccgccatac tgttaaaagg caaaaaattg aagatcgata gggtgcccaa tcttctggat
     3481 gttgtgacca tggcagatct tcttcgaagt ttaggagctg tggtgcagtt cagcgatcac
     3541 gagatggaga tctccactcc tgaagaaatc tcatgggaga ctcctccgga tcttgttcgc
     3601 aagatgaggg cttcgtccct tgtccttggt cctttgcttg cccggtgcgg caaggccgtg
     3661 atgccgttgc ccggcggttg ttcgatcggc agtcgtccca ttgacctcca tcttaagggc
     3721 ttgacccgga tgggcgcgtc gattgagctt gtccacggtg cggtacatgc gcgggcggat
     3781 ggattgagag ggtgcagaat ctatcttgac ttcccgtcag tgggagctac agagaacctg
     3841 atgatggccg ctgtgtttgc tcaaggggaa accgttctcg aaaatacggc gagagagccg
     3901 gagattttca accttgtcga ggcgcttcgt tcgatggggg cttttattga tgccgacgga
     3961 accggtgtga tccggatcaa gggagtggac gaccttgaaa gcgcctccgc gaccgtcatt
     4021 cccgacaggg ttgaggcagc gacatatatt cttgccgggg cggctacaaa tggagaggtg
     4081 acggtttccg acatcatacc gaaccatatc gattcacttc ttgctaagct tgaagaggcg
     4141 ggcgccttga tgacaatcaa ggaaacggag gtgacaatac acccttcggg aaggctgaag
     4201 gcagtctctt tgaaaaccct tccctatccc ggtttcccga cagatcttca gcctcaggtt
     4261 atggttgcat tgtgtcttgc ggaaggaacg agcgttgtcc aggaaagcgt ttttcagtcc
     4321 aggttcctcc acgttagcga gctcaacaag atgggggcca aaatcgacat tcagagcaac
     4381 tccgctatta tcaacggcgt agcatccctt gcaggagccg atgtcctcgc gacggacctg
     4441 agggcggggg ctgctcttgt actggcgggg cttgcggcaa gagatattac ctatgttcat
     4501 catctcgggc atatttcacg cgggtatgaa cttatggaag aaaagttgcg cgcgatcgga
     4561 gcaaatattt cgattctccc atccgtagag gattccaaag gggattctcc ggcgcttcgt
     4621 tcttaaagcc ggccggaatg tctcttttcc tgctctatgg tatgtcgtca tggcgttaaa
     4681 cgattccggt gtgacaagag ttattgcttt tgtgggtcct gcaggtaccg gtaagagtca
     4741 acgggcgcag atggtcgccg tggaaaatga ggttgactat ataatcgacg acgggcttgt
     4801 tattgccaag ggacgtataa tggtgggaaa gagtgcaaag gctgagaaga accttgtgcg
     4861 tgcaatacga agagctcttt ttcagttccc ggagcatagg aaagcggtat tggccttttt
     4921 ccagaagaaa caaccgaaga agctgatgat tattgcaacg tcgatttcga tggcagagaa
     4981 gatcaccaga gcgcttcgcc ttccgtatcc ggaaatgatc atcgatataa cggaggtagc
     5041 ttcggcagaa gaaatcatca atgccagaag agagcgtcat gaaaaaggac agcacgttat
     5101 tcccgcctcc aggacgcaga tacggaaaaa ttttgccgga aaactagttg gacatctgcg
     5161 gggactcttt aaaagcgtcg acaaagagga cggcgaacgg acaatcgtac gccctccgtt
     5221 cagtttttat ggagagctga cgatagagtc ttcggcaatt cttgatctcg tccgccatgt
     5281 tctttcttct aatccgcaga ttacggaagt gaaggatatt cgtgttcgat ataaagatga
     5341 cgctatttct atggagattg tacttcaggt tattccgggg aaaatggatt tccaccgact
     5401 tggtgtagtc aatcaaaaga gagtagcatc cgcagtcagc tactttacgg gaatggatgt
     5461 ggacggtgtc gacgtccaca tagtggaggt aatgctgcca tgagcgtctt tgcgagcgga
     5521 gccgacagcg ggttggcatg ggaggaactg aaaaggcgtg tagaaacctg ttcatcgtgc
     5581 ggcctgtgtt cgacgcgaaa gaagacggtt tttggtcagg gaagcgagac ctccccggtt
     5641 gttttcgtcg gcgaaggtcc cggagcggac gaggatgaac aaggagtccc atttgtagga
     5701 cgagcgggaa aactgctcac gcgtattctt caatcggtcg gtattgagag ggaaagcgtt
     5761 tttattacga atgttgtaaa gtgccggccc ccgcagaacc gcactccgat ggcggacgaa
     5821 atgatggcat gcacgccatt tctggaggcg cagatggccc ttctccggcc gaagatcgtg
     5881 gtgtgcctgg gaaacactcc ggcaaaatgg ctgctcaagt cctcagaagg catttccgca
     5941 cttcgcggac gttggtttgc atggagggga atcgatcttt tcccgatgtt tcatccaagc
     6001 tatcttctcc gcaatgaatc aaagaagaag ggaagtccga aggatttgac atggcaggat
     6061 atcaacgctc tccgcagcaa actcgatgat ctgacgaaag gagggccgtc cgcatgatac
     6121 cgaaggagac agtacctcgc cacgtcgcca taataatgga cggcaacggg cgttgggcaa
     6181 aaaaacgctt tcttccgcgt ctgctggggc ataaagcagg agtgaaggct attgaacgga
     6241 cggtacgggc agccgacgcg cttggtattc aatatgtttc tctgtatgct ttttcaacag
     6301 aaaactggaa aaggcctgaa aaagaggtct caggtcttat gagtatcttc cgttttgtct
     6361 tgcgccgaaa agttgatgaa atgaagcgtg agaatgtgcg tcttcgcttc tccggttttc
     6421 gtgacgggat gccgcaggat atcgtcgagc tgatggagtg ggccgaagaa gaaacaaagg
     6481 ggaatagcgg tctcacgctg gtcccctgtc tcaattacgg cggccgccag gagattctgc
     6541 acgcagtgga gaagctcctg gaagatggcg cccgccctcc tttgaccgag gagcgtttca
     6601 gagaatacct ctatcttccg gatcttccgg accctgatct cattgtccgg acaagcggcg
     6661 agagtcggtt gagtaacttc tggctctggc agagcacgta tagcgagctg tattttacgg
     6721 acaccttgtg gccggatttt gacggaaagg aacttgaaaa cgccgtgaaa tattttgcga
     6781 atagagagag acgctatggc gctctcaagt gaaaagctac gggaacttac tctgcgaggg
     6841 ggaagttctc tcgtcatcgt ggcaggtacg ctcagttccc tctatgtcgg cggtatggtg
     6901 tggacatttt ttgcgtccgc tgttgctttg atctctctcg ccgaattcta cgggatgctt
     6961 tccaggaagt tcaaggtctc caagggaata ggcttcctta cctcggggct tattatcttt
     7021 tcagtatcag aggggattca cccggtgtcg ctcgttctca cactgagcgt atcggctttt
     7081 gtcgtgcttt ttgtagaaat aatccgcagg cagcttcgag gggaaagttt tgcaatttgg
     7141 aatatggggg gtacaatttc gggtatagta tacatcacta ttccgtggac cttcatgatt
     7201 ctgttgcgac gcctccccgg aggagggctc ttattgctgg cgatgttcat cgctacatgg
     7261 agctgtgatg tcgcggccta tcttataggg tcgcgctggg ggcgcactct cctctgtgtc
     7321 aacgtgagcc cgaacaaaac gtgggaagga ttcttcgggg gcttttctgc ggcgctgctc
     7381 gcaagtgtgc ttttggcgtt catactcgag ataccgccga ttccatttgt ctacgtaggg
     7441 ataatctgcg gttttgcggg acagctcggc gatctcgcag agtcggttat caagagagaa
     7501 agctgggtaa aggacagcgg aaccctcatt ccgggtcacg ggggtgtgct cgacagattt
     7561 gacagcacgc tgatcaacgg tctgttggtc tatgtacttt tcggggtgat cctgcgatga
     7621 agaaacgtct ttttgtcatc gggtgtacgg gcagcgtcgg aagctctgtc ctctccgttt
     7681 gccgtatgtt tcccgagcac ttcgaaattg cggctctggc agcgtgctct tcggtggatc
     7741 gtatgcttct tcttgcagag gaattttcct gcccgacgct tgtcttttcc gatgaatccg
     7801 cggcagttcg agctgcaaac gaactgctct cttcgggcgt tcaatacgcc gggagaagag
     7861 tccttgccgg agaacaggga cttctggaaa tggcatcgca ccccgatatc gaccacgtgg
     7921 tttttgcctc ttccggaaca ggggcaatag gcgctctcat ggcggcgctc agtagcggca
     7981 aggaggtctc cctggcaaac aaggagagca ttgtcgtcgc cggtccgtgg gttctccctc
     8041 tggttcttcg ccccgatcag atccgtccgt tggacagcga acacaacgct atttggcagt
     8101 gcctttccgg ggaagatcgg cgttttgtga acaaaatttt tctcaccgca tcaggcggtc
     8161 ctttccgctc tttttctcaa gaggagatga agcgggtgac gccttcgatg gctgtcgcgc
     8221 accccgtatg gaacatgggc gcaaagataa gcgttgacag cgcgacctta atgaacaaag
     8281 ggattgaaat tcttgaggcg atgtaccttt tttctttttc cgctgaccag gtggacgcgg
     8341 ttatctctcc gaaccctttc attcacgggc ttgtttgttt ttcagacggg acttttcata
     8401 tgtgcgcgtc cgccccggac atgggcatcc ccgccttatc ggctcttttt tcgcctcttc
     8461 gcagtccgcg cgacagaaag aacacccctg ttttggatgg aatgcagatc tcttttgagg
     8521 cccccgacga ggtcagattc ccatgtattc gattggcacg tgaagttgcc cggaaggaag
     8581 gcgcctaccc ggcgctcctg gtaggagcgg atgaggttgc tgtcgaccgc ttcctttcag
     8641 gccgcgtcag cttcatgagt attcctcgta tcgttgaatc cgtgcttgag gagtattccg
     8701 gaccgtcccc ctcgacactt gagcatgctc tgcatattct tgaagaagga aggcggatgg
     8761 cggaaaaggt gagttcacga ttggaagaaa aataatggtc aaggagataa gataaatggt
     8821 gagtttggtt tcgttcctac tggtaatcgg tatttgtgtc attatccacg agcttggaca
     8881 ctattttaca gcgcgcatat tcgatgttca ggtgcatgag tttgctttcg gtatggggcc
     8941 agtgctcctt cagcggcaag gtcgccatac catgtggtct tttcgagcat ttcccgtcgg
     9001 ggggttcgtc cgtcttgcag gaatggggga ggaccacgaa gaggaagttg tcgaggaggg
     9061 aagagggttt ttcgacaagg ctgcctggaa gcgttttctg attcttgcga atgggtctgt
     9121 gtccaacatt cttcttgcga tgcttctgac ggttttcttt ctctcgggcc acggtgtgat
     9181 caatcttgag agcacagaaa tcggcgagat tatggagggg taccccgcgc aaacggcggg
     9241 tattcttccc ggcgacgcca ttgtcgctgt caacgacgta cctgtttctt catggcggga
     9301 aatgtctgag agtattcgta caacagcctc ggaaggacct gttctttttt cggtgttgcg
     9361 gaagggagaa accctttctt tgacagtaag cattccgccg g
//
LOCUS       DAOSWW010000007         9237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_4777_length_9237_cov_3.979634, whole genome shotgun sequence.
ACCESSION   DAOSWW010000007 DAOSWW010000000
VERSION     DAOSWW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_9237_cov_3.979634"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PK442_00290"
     CDS             complement(<1..616)
                     /locus_tag="PK442_00290"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO86118.1"
                     /translation="MSKTSLPSQEPYSAVSGVNGTERRAWGTFVSTFLQTMAPVFFLI
                     VFLLVLLLYFFLPLLEESYLHQKRLMCKYLVQVQMTYLEALNTDVLKGTISMEEAQGR
                     ALRRLRMHRFGEAEKDYFWALGPERTLVMHPYRPDLEGINPDEAKGPDGELLRGLFDG
                     IEAAAARTPEGGHLDYLWHFKDDLGTLSAKTSHVARFVPWNWIVG"
     gene            complement(834..1574)
                     /locus_tag="PK442_00295"
     CDS             complement(834..1574)
                     /locus_tag="PK442_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOO86119.1"
                     /translation="MIATPIAFLAGETELRREKPELVKAVIFDLDGTLIDSEENHYES
                     DRVLLARRGVTFSREDKKAFVGKDVTEMVRRIKANYGLASDAQSLMDEKNAIYRSLAL
                     KSSRLYPPMKVVLEEFRKRKLPMAIATGSNSSIAGEILTSLGVRDFFTMIVSSSEVKK
                     GKPEPDIFLETARRMAIDPKEAIVFEDTRFGVEAALRAGMTCVALPAPEDDADHKLFQ
                     QADCLIKGGPDELDPQSFFWWFDTLAKL"
     gene            2017..4077
                     /locus_tag="PK442_00300"
     CDS             2017..4077
                     /locus_tag="PK442_00300"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO86120.1"
                     /translation="MKNRKGIFSSRRLSLMAKLGGGTALLVIVVVAITAGASLSRFSE
                     VLEENAHQKSILAAHGLESILEAKTAESRMATLFLVSAPGLADTVKAGNSKALAEQLT
                     PLWKEQTDLDFAAVLDPKGTVLLRLHEPDKNGDSLADEFNVQGAMKGQTTSAIEPGTQ
                     VPLSARTATPIKDASGALVGILTTGISFSREAFVDQAKELFDAEVTVFAGDTRLMTTI
                     RRDGERVVGTKLDPAIAAVVLSGKSYFGEAEILGAPYITAYEPILGPDGKPVGIYFSG
                     ISMAALATVQQSTFWVALGVAAVGLLFLGLLVRKIVKSIRTMVFFMKEVEAGRLYHTR
                     EEFGVTSHDEIGDMADALAEMVRVQREMISELKEKSVHLSALSEETAASTEEVTTTTN
                     EVAESNSKLAEQTRNGRLNAVESSKVMLEMSSLIQIAQTLASSADKNSGGMSEAVLEG
                     RETVFRTIERIEGVKGAVEETENVLSQLDAYSQRIGVVGDTITGIADQTNLLALNAAI
                     EAARAGEAGRGFAVVAEEVRKLAEQSQQGAREVSELVGKILDGTNLAVASMRKSREGV
                     EEGVSVAHVAGSALERIKEAIDKSIADIRRIIDTTNEEVARSDKVIKLIDTTASVMEN
                     TDDYVQNLAASMEETAAAMENVATSANEVSESSEELRRMTDRFQVEKDGGGTTSAIVP
                     KRGA"
     gene            4298..6310
                     /locus_tag="PK442_00305"
     CDS             4298..6310
                     /locus_tag="PK442_00305"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO86121.1"
                     /translation="MKANERGIAERTKHGRRLSLMAKLGGGTALLALLLVAVTAGSAV
                     VIFSNYLDRDAREKSEAAVRGLESILEAKREETRVVSLFLARYGNIVEDVAAGNADAL
                     LQKLTPMWKESDLDFVTVLDAKGNVVVRLHEPDKKGDSLAGQANVKGALRGEITTAIE
                     PGTQIPLSVRTGVPLKNASGAVVGALSAGISFSREVFVEQAKEMFGAEMTIFAGDVRL
                     MTTIVQDGKRVVGTKLDPAIAAIVLGGASYNGAANILGAPYVTAYDPIEGPDGKVVGI
                     YFSGIPMTEVNAARWAVVRTVLLLAGALLVLGVLLQGWFVRKIVRSIRTMMGFMKEAE
                     TGTLCYTREDFGIRSRDEIGDMADALGSMMEKLCTVIGELRGDAQGTAQRAQALAALS
                     EESLASMEEIGASVERANALSEHSASAAARSSEEGAEAAGQTKEISERAVGEVEHSIT
                     LIREGGRKVNQTAREMENVSRSVESISGFVATITSIADQTNLLALNAAIEAARAGEHG
                     RGFAVVADEVRKLAEQSGHAAQEVQKLIAGLQAGAKSSLAVTGEAEEIMKQTVSSAEE
                     ALTELRKAMDQIARTSDAVQGIAAAAEEQAASAQQMTAGVEQVTRSTSEVMDALHIIQ
                     SASGETVKASQDLAAEAQELLANAEKIEALLSDFTVVKEKAGLLPR"
     gene            6487..7662
                     /locus_tag="PK442_00310"
     CDS             6487..7662
                     /locus_tag="PK442_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO86122.1"
                     /translation="MKLSMRKCLLIVCFLPFFISSASAAVVPFPELCASGTPAQIRAA
                     ILDGADIGERNSEGVTPLMFAAAGNPDPLAASELLDAGASLTERDYAGLTPLMWAAAR
                     NEEPQVSALLLQRGAEVNARSRDGVTPLLFAAGNNENPEIASLLLKAGADKDAKNRSG
                     WSAFMFAAANNPNPEILRVLRSSGADPNARSADGWTPLMRAAAHTSAPEIVREFLRFG
                     ADANARGGEQETPIMAAARFNTNAAVLRELLQNGADPNARDKNGVTVLMWAAANNLNP
                     EIVSVLAKAGAYPDARGKNGDTAIMGAAMSNPNPQVVAALVEAGADVNMKGPRDWTAL
                     MFGALHTRNPEVVSSLLAAGANATIQNRDGGRAIDYAGKNPHLKGTKAYWLLNEASY"
     gene            complement(7687..8061)
                     /locus_tag="PK442_00315"
     CDS             complement(7687..8061)
                     /locus_tag="PK442_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86123.1"
                     /translation="MLIVEDQRLNRMLLKKLVDALLPSATVYEAVDGVEGVELFERHA
                     PDIVFMDLQMPRKDGFHAATEIRTLEADGRRTLIIALTADAQPETRESCLESGMDDYL
                     TKPVSIHDLRRVLEHGAMRTEE"
     gene            complement(8019..>9237)
                     /locus_tag="PK442_00320"
     CDS             complement(8019..>9237)
                     /locus_tag="PK442_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86124.1"
                     /translation="MALKIINNAMLLLLLQTALSVLLATIAALAFHASFGRPLSRFAR
                     FISADESSLSGEPFVLESRMHDRDELGILVSSFNRQHSATARSIAEINEMNRRLSDAN
                     ALLRQSEEALRTSKESAEWLGRQAESANRAKSVFLANMSHELRTPLNSVIGFLDLLGV
                     TPLDEKQREYLGYVRTSAHSLLDVISNILDISKIETGKLELKPEFTNIRRLLEQAMNI
                     ARGTAVEKGLHLVLRVEDATPRRIFVDAIRLKQVLVNLLGNAVKFTGKGEVELSLRFE
                     ETGNETGSFLFSIRDTGIGIPEEKQERLFEPFYQGDESITRQYGGTGLGLPICDALRR
                     GWEARLNWRACPEREAASSSRSPPDTPTTRRSKNPLTLRDTPPPTASSSRFTPRSGLR
                     CSSSKTRGSTGCC"
BASE COUNT         1893 a         2582 c         2983 g         1779 t
ORIGIN      
        1 ttccgacgat ccagttccac ggaacgaagc gggcaacgtg cgaggtcttg gccgagagcg
       61 tccccagatc gtccttgaag tgccacagat agtcgaggtg tcctccctcc ggggttcgcg
      121 ccgccgccgc ctcgatgccg tcgaagagac cgcgcagcag ctccccgtca ggccccttcg
      181 cctcgtcggg gtttatgcct tccaggtcgg gacgatacgg atgcatgacg agcgtccgtt
      241 ccgggcccag cgcccagaag tagtcctttt cagcctctcc gaagcgatgc atccgaaggc
      301 ggcgaagggc ccgtccctgc gcttcctcca tggagatcgt tcccttgagg acgtcggtgt
      361 tgagcgcttc gaggtaggtc atctggactt gcacgaggta cttgcacatc aggcgcttct
      421 ggtgaaggta gctttcttcg agcagaggaa ggaaaaaata aaggagcagg acgagaagga
      481 agacgatcag gaaaaagacg ggagccatgg tctgaagaaa cgtcgagacg aatgttcccc
      541 atgcgcggcg ctctgtcccg ttgacgcccg aaacagcgga gtacggttct tgagaaggaa
      601 gcgacgtctt ggacatctcc aggaatctcc tttctcctgt tacataccgc cctgagcatg
      661 gaggttcaca aagattgtat cagatgcgtt cggctgcgtg agaatcttga ggctcatatc
      721 tgttccggag agaggcgcaa cttgcagttc atgcaaaaaa tctgcggggc gacgcgggga
      781 tctgccgatg gacggtacgc gtcgcctctg cggatggggg tgcggagcgt gaatcagagt
      841 tttgcaaggg tgtcgaacca ccagaagaac gattgcggat ccagctcgtc cggcccgccc
      901 ttgatcagac agtctgcctg ttggaagagc ttgtggtcgg cgtcgtcttc cggggcgggg
      961 agggccacgc aggtcatgcc cgcgcgaagg gcggcttcga cgccgaaccg ggtatcctcg
     1021 aagacgatgg cttccttcgg atcaatggcc atccggcgcg ccgtttcaag gaaaatgtcg
     1081 ggttcgggtt tccccttctt cacttcggag gaggagacga tcatggtgaa aaagtcgcgc
     1141 acgccgagcg acgtcagaat ctccccggcg atggaggagt tggagccggt cgctatcgcc
     1201 atggggagct tgcgcttgcg gaactcctcg aggacgactt tcatgggggg gtacaggcgg
     1261 ctggatttca gcgcaagcga gcggtagatg gcgttcttct cgtccatcag gctctgcgcg
     1321 tcgctcgcaa ggccgtagtt ggccttgatc cgccggacca tctcggtgac gtccttcccg
     1381 acgaaggcct tcttgtcctc ccgtgaaaaa gtgacgcccc ggcgcgcaag gagcacccgg
     1441 tcgctctcgt aatggttctc ctcgctgtcg atcagagtgc cgtcgagatc gaagataacc
     1501 gccttaacaa gctccggttt ttcccggcgc agctccgttt cgcccgcgag aaatgcaatt
     1561 ggtgtcgcga tcattgtgat tctcccttcc cgaatgctct ggtccggaag aattgtaaca
     1621 tggaatttct ttcaggaaaa tacgatctcc acctgaattc aactctgcat gttgttgcgc
     1681 ctccgtccgc cgcagtgcgg cggaaaaacc gaaaaatagt atgcaaacga agatgcaatt
     1741 gaaatagtcg atagcaagag ccgataaaga gaatggaagg atattccttt tctttaaggt
     1801 ttcttcgaga tgagaagcca agttgtaata ttttgttact ttcaagtgtg cgcttttctg
     1861 agttcgttat aaatttattt ataaatggaa ctatagaaaa ttaacaaaca cattaaagct
     1921 catttatata atccgctgta atgaattgtc tttcgagaag aagatgttac aatgtataaa
     1981 ctggctttta aattttcatt tcacggaggg gaaaaagtga agaatagaaa aggaatcttc
     2041 tcgtcgcggc gtctctctct tatggcgaag ctgggcggag gaacggccct gctggtcatt
     2101 gttgttgtgg ccattactgc gggcgcctcg ctcagccgtt tctcggaggt cctggaggag
     2161 aacgcgcacc agaagtccat tctcgccgcg cacggactgg agagtattct cgaggcgaaa
     2221 actgcggaat caaggatggc gacgctcttt cttgtctccg ccccggggct tgccgacacg
     2281 gtgaaggcgg ggaattccaa ggctctcgcc gaacagctga cgcccttgtg gaaagagcag
     2341 accgatcttg acttcgcggc ggtgctcgat ccgaagggca cggtgctcct ccgtcttcac
     2401 gaacccgaca aaaacggcga ttcgctggcc gatgagttca acgttcaggg cgccatgaaa
     2461 ggacaaacca ccagcgccat agaacccgga acgcaagtac ccctctcggc gcgaaccgcg
     2521 accccgatca aggacgcctc cggagcgctc gtgggcattc tcaccacagg catttcattt
     2581 tcaagagagg cgttcgtcga ccaggcgaag gagctcttcg acgcggaggt taccgtcttt
     2641 gccggggata cccgtctgat gaccaccatt cgccgcgacg gggagcgtgt tgtcgggacg
     2701 aagctcgacc cggcgatagc agccgtcgtg ctctcgggga agagctactt cggcgaggct
     2761 gaaatactcg gcgcccccta catcactgcg tacgaaccta tccttggacc cgacggcaag
     2821 cctgtgggca tttacttctc cggtatttcc atggcggcgc tggccactgt gcagcagtcg
     2881 actttctggg tagcgctggg ggtggcggca gtcggcttgc tcttccttgg gttgctggtc
     2941 cggaagatcg taaagagcat ccggacaatg gtcttcttca tgaaggaggt cgaagcgggg
     3001 cgcctctacc atacaaggga ggagttcggc gtgacctctc acgacgagat cggcgacatg
     3061 gccgacgctc ttgccgaaat ggtccgcgtg cagcgggaga tgatcagcga actgaaggag
     3121 aagtccgtcc atctttcagc cctttcggag gagaccgctg cctccaccga ggaagtgacc
     3181 accacgacca acgaagttgc cgagagcaat tccaagctgg ccgagcagac gaggaacgga
     3241 cggctcaacg ccgttgaatc ctcgaaagtc atgctggaga tgagcagcct tatccagata
     3301 gcgcagaccc ttgcgtccag cgcggacaag aactccggag gaatgtcgga ggctgtgctc
     3361 gaaggccggg agacggtgtt cagaaccatc gaacgcatcg agggagtcaa gggggcggtt
     3421 gaagagaccg aaaacgtgct ctcgcagctc gacgcctact cgcagcggat cggcgtggtc
     3481 ggcgacacca tcaccggtat cgccgaccag acgaacctgc tggcgctcaa cgccgcgata
     3541 gaggctgcgc gggcagggga agccgggcgc ggattcgccg tcgtcgccga agaagtacgc
     3601 aaactcgccg aacagtcgca gcagggcgct cgcgaggtgt cggaactcgt cggaaagatc
     3661 ctcgacggta cgaacctggc ggtagcgtcg atgcgcaaga gtcgcgaggg agtcgaggag
     3721 ggcgtttccg tcgcgcacgt tgcggggagc gctcttgagc gcatcaagga ggccatcgat
     3781 aaatcgatag cggatatccg ccggattatc gacaccacca acgaagaggt cgctcgatcg
     3841 gacaaggtta tcaagctcat cgacacgacc gcgagcgtga tggagaacac cgacgactac
     3901 gtccagaacc tcgcggcctc gatggaggag acggccgccg cgatggagaa cgtcgcgacg
     3961 agcgcgaacg aagtcagcga atcctccgag gagctcagac ggatgaccga ccgtttccag
     4021 gtagaaaaag acggcggggg aacaacgtcc gcgatcgtcc cgaagagagg agcctgagcg
     4081 gcctccggag agtcccgtcc tgagaaagaa cgagatgccg gggtgttcgc cgcaaaggga
     4141 acgctccggc tttttttggc acgcgcgctg tccgattatt tggcggagtc gaatgactct
     4201 tcgtcaatac atttgttcaa ggggaataat tgcctctgaa tggatgtagt cttagtaaag
     4261 aacagatatc tcaagctgga aaggcggagg aaaagcaatg aaagcgaatg aacgtggtat
     4321 agcggaaagg acgaagcacg gcaggcgcct ctcactgatg gcgaaactcg gcggaggcac
     4381 ggcgcttttg gcgctgcttc tggtggcggt aacggctggt tccgccgtag tgatcttctc
     4441 gaattacctc gatcgggatg caagggaaaa atccgaggcg gctgtccgcg ggcttgagag
     4501 catccttgag gctaagcgcg aggagacccg ggtagtcagt ctcttcctgg ccaggtacgg
     4561 gaatattgtg gaggacgtag cggcggggaa cgccgacgcc ctgctgcaaa agctcacccc
     4621 gatgtggaag gagagcgacc tggatttcgt caccgtgctc gacgcgaagg gaaacgtcgt
     4681 cgtgcggctg catgaaccgg acaagaaggg cgattcgctc gccgggcagg cgaatgtgaa
     4741 gggcgcgctg aggggagaga tcaccaccgc gatagagccc gggacgcaga ttccgctttc
     4801 ggtgcgcacc ggcgtgccgt tgaagaatgc ctccggcgcc gtcgtcggcg cgctctccgc
     4861 agggatctcc ttttcgagag aggttttcgt cgagcaggcg aaggaaatgt tcggcgcgga
     4921 gatgaccata tttgcgggcg acgtccgcct gatgacgacc atcgtgcagg acggcaagcg
     4981 cgtggtgggg acgaagctcg atcccgccat agcggccatc gtgctcggag gagcgagcta
     5041 caacggcgcg gcgaatatcc tcggcgctcc ctacgtgact gcgtacgacc ctattgaggg
     5101 cccggacgga aaggtagtcg gcatctactt ctccggcatc ccgatgaccg aggtgaacgc
     5161 cgcccggtgg gcggtcgtcc gtaccgtgct gcttcttgcc ggagcgctcc tcgtgctcgg
     5221 cgtgctgctt caagggtggt tcgtcagaaa gatcgtccgg agcatccgca ctatgatggg
     5281 attcatgaag gaagcggaaa ccgggacact ctgttacacc cgggaggact tcggcatccg
     5341 ctcgcgggat gaaatcggcg acatggcgga cgccctcggt tctatgatgg agaagctttg
     5401 tacagtcatc ggcgagcttc gcggggatgc gcagggtact gctcagaggg cgcaggcgct
     5461 tgccgcgctc tccgaggaga gccttgcgtc gatggaggag ataggggcct ccgtcgagcg
     5521 cgcgaacgct ctctccgaac acagcgcttc cgcggcggcg cgttcctcgg aagagggcgc
     5581 cgaggctgcg gggcagacga aggagatcag cgagcgcgcc gtcggagagg ttgaacattc
     5641 catcaccctc atccgggagg ggggccgaaa ggtcaaccag accgcgaggg agatggagaa
     5701 cgtctcccgt tcggtggagt ccatctccgg ttttgtcgcc accatcacgt ccatcgccga
     5761 tcagacgaat ctgctggcgc tgaacgccgc catagaagct gcgcgcgccg gagaacacgg
     5821 ccgcgggttc gccgtcgtgg ccgacgaggt gcgcaaactg gccgagcagt ccggtcatgc
     5881 ggcccaggag gtacagaagc tcatcgcggg gcttcaagcg ggagcgaaga gctcgctcgc
     5941 cgtcaccggc gaggccgagg agatcatgaa gcagactgtc tcttccgcgg aggaggccct
     6001 aacggagctc cggaaggcga tggaccagat cgcgagaacc agcgacgccg tgcaggggat
     6061 cgccgccgcg gcggaagagc aggccgcgtc tgcgcagcag atgacggcgg gcgtcgaaca
     6121 ggtgacccgt tcgacgagcg aggtcatgga tgcgctgcac atcatccaga gcgcctcggg
     6181 ggagactgtg aaggcctctc aggatcttgc ggccgaggcg caggagcttc tggcgaacgc
     6241 tgaaaagatt gaggcgctgc tctcggattt taccgtcgta aaggaaaagg cggggcttct
     6301 gcctcgctag tctcttttca gaagcgcgta acaggtccgg atatgagata cccgcttcat
     6361 acgcccgcag tcgcccgcaa agcgattcgc gggcgttttt aatagcgcag tcgcgtggaa
     6421 taaaaggact cttttttcgg tggtatcatg ttttcgatgc acatatgttc gggaggaggg
     6481 agttgtatga aattgagcat gcggaaatgc ttgctgatcg tgtgttttct tccgttcttt
     6541 atttcttctg catccgccgc tgtcgttccg tttccggagc tgtgcgcctc cggaacgccc
     6601 gcgcagattc gagcggccat tctggacggc gccgatatcg gcgagcggaa cagcgaaggc
     6661 gtgacgccgc tgatgttcgc ggccgccggt aatcctgacc cgcttgcggc ctcggagctg
     6721 ctggacgcgg gagcgtcgct caccgagcgg gattacgcag ggctgacgcc gctcatgtgg
     6781 gcggcggcgc ggaacgagga acctcaagtt tcggcgctgc tgttgcaacg gggcgcggag
     6841 gtgaacgcca gaagcaggga cggtgtgacg ccgctgctct tcgccgcggg gaacaacgaa
     6901 aatccggaga tcgcgtcgct cttgctgaag gcgggcgccg acaaggacgc gaagaacagg
     6961 agcggatgga gcgcgttcat gttcgcggcg gcgaataatc cgaacccgga gatcctccgg
     7021 gtgttgcgca gcagcggcgc ggatccgaac gcgcggagcg cggatggatg gactcccctg
     7081 atgcgggcgg cggcgcatac gtccgctccg gagatcgtcc gcgaattttt acgtttcggc
     7141 gcggatgcga acgcacgcgg cggcgagcag gaaacgccga tcatggccgc ggcgcgtttc
     7201 aacaccaacg cggcggttct ccgcgagttg cttcagaacg gggcggaccc gaacgccagg
     7261 gacaagaacg gcgtcacggt gctgatgtgg gctgctgcga acaacctgaa tccagaaatt
     7321 gtgagcgtgt tggcgaaagc cggggcgtat ccggacgcga gggggaagaa cggcgacacg
     7381 gccatcatgg gagcggcgat gagcaacccg aatccgcagg ttgtcgccgc gctcgtcgag
     7441 gccggggcgg acgtgaatat gaagggaccg cgggactgga ccgcgctgat gttcggggcg
     7501 ctgcacaccc ggaaccctga agtggtgagc tcgttgctgg cggcgggggc gaacgcgacg
     7561 atacaaaaca gggacggcgg acgcgccatc gactacgccg gaaaaaatcc gcacctcaag
     7621 ggaaccaagg catactggct tctgaacgag gcctcgtact gagaggcctc gtactgaatc
     7681 gaaggattat tcctccgtcc tcatcgctcc atgttccaag actctccgga gatcgtggat
     7741 gctcaccggc ttggtgaggt aatcgtccat tcctgattcg aggcagctct ctcttgtctc
     7801 cggctgggcg tcggcagtga gcgcgatgat caatgttctc cgtccgtccg cttccagagt
     7861 ccgtatctcc gtcgcggcat ggaaaccgtc ctttcggggc atctgcagat ccataaagac
     7921 gatgtccgga gcgtggcgtt cgaagagctc cactccctcc accccgtcga cggcctcgta
     7981 gacggttgcc gacggcagca acgcgtcgac cagttttttc aacagcatcc ggttgagcct
     8041 ctggtcttcg acgatgagca ccgtaggccg ctccggggtg tgaatcgcga agaggacgct
     8101 gtcggagggg gcgtgtcccg aagcgtcaaa gggttcttcg atctcctcgt cgtcggcgta
     8161 tccggcggcg agcgtgaaga agaagcggct tccctctccg ggcacgctct ccagttcaag
     8221 cgcgcttccc atcctctgcg gagcgcgtcg cagatgggaa gtcccagccc cgtccctccg
     8281 tactgtcgtg tgatcgattc atccccctga tagaagggct cgaagaggcg ctcctgtttt
     8341 tcctccggga tgccgatgcc ggtgtcccgg atggagaaga ggaagcttcc cgtttcgttg
     8401 cccgtctcct cgaagcggag cgagagctcc acctcccctt tcccggtgaa cttgaccgcg
     8461 ttgcccagaa gattgaccag cacctgcttc aagcgaatcg cgtcgacgaa gattcggcgg
     8521 ggcgttgcgt cctcgacccg gagcacgaga tggaggccct tctctacggc ggtaccccgt
     8581 gcgatgttca tcgcctgttc gagaagacgc cggatattcg tgaattcagg cttcagttcg
     8641 agcttgcccg tttcgatctt cgaaatgtcg aggatgttgc tgataacgtc gaggagggag
     8701 tgtgctgacg tcctgacgta ccccaggtat tcgcgctgtt tctcgtcgag gggggtgacg
     8761 ccgagcagat cgaggaagcc gatcacgctg ttcagcggtg tgcgcagttc gtggctcata
     8821 ttcgcgagga agacgctttt cgccctgttc gcgctctcgg cctgtctgcc gagccactcg
     8881 gcgctctcct tggacgtccg cagcgcctcc tccgactgac ggaggagtgc gttggcgtcg
     8941 ctcagccgcc tgttcatctc gttgatttcg gcgatggagc gggccgtcgc gctgtgctgc
     9001 cgattgaagc tcgacacgag gatccccagt tcgtccctgt cgtgcatgcg actctccaga
     9061 acgaacggtt ctcccgacag agaactttca tccgcggaga tgaagcgcgc gaagcgggaa
     9121 agcggcctcc cgaaggaggc gtgaaacgcc agcgcggcga tggtcgcgag cagaacggaa
     9181 agagctgttt gcagcagcag aaggagcatt gcgttgttga tgatcttcag cgccatt
//
LOCUS       DAOSWW010000008         8931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_4996_length_8931_cov_3.816021, whole genome shotgun sequence.
ACCESSION   DAOSWW010000008 DAOSWW010000000
VERSION     DAOSWW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8931
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4996_length_8931_cov_3.816021"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /gene="pyrB"
                     /locus_tag="PK442_00325"
     CDS             complement(<1..264)
                     /gene="pyrB"
                     /locus_tag="PK442_00325"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048980.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOO86125.1"
                     /translation="MEWRHKDLISLEGWTREELHFFLEQADYMEELMNRPIKKVPALR
                     GKMVVNCFFENSTRTRVSFELAEKMLSADVVNWSASGSSTSKGE"
     gene            complement(248..793)
                     /gene="pyrR"
                     /locus_tag="PK442_00330"
     CDS             complement(248..793)
                     /gene="pyrR"
                     /locus_tag="PK442_00330"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOO86126.1"
                     /translation="MTERDMERVLRRIAVEIVERNKGLDDVVLLGIQRRGVHLAARLR
                     EIFKAEEKVKVPAGELDITLYRDDISTLADQPVVHSTSLPGDITGKRVVLVDDVLFTG
                     RTIRAALDAITDLGRPERVQLAVLVDRGHRELPIAADYVGKTIPTSRSENVEVRVKEL
                     DGTDGVVICERNGGAEHGVEA"
     gene            complement(1055..2734)
                     /locus_tag="PK442_00335"
     CDS             complement(1055..2734)
                     /locus_tag="PK442_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO86127.1"
                     /translation="MPEERGSVLKKYAESGLCRLLLDMLRIPSVSPSPEHENRVTRLL
                     YDTLAVLPYFREHPEDLRLLPLEDDPFGRNFLFAFVRRPGPTAETVILAGHADVVSAE
                     ACGELAPFAFDPEEYTRRLEDVALPEDARRDLESGEWLFGRGVADMKSGLAAGIELLA
                     EAASDPGSLGANLLALAVPDEENNSAGMLAASAQLARRQKEEGLRYRACIELEPTFAG
                     GERALPSIYLGSIGKINTFFFCAGKETHVGEYYEGFGAAPVISRINLELEGNPEYADS
                     LGGRAYPPFGCMRQTDLRREYSATIMTRAFSFYSYLTATKLPGRILGEMRAIAEKALR
                     EAIEAHERNAESFAKMNGAGRSDAKWNSMALSYEELRRMAEAKLGAGFPGFVEEVLSR
                     TPSGADERTKAVALVESMVEACALPGPLVVFGFLPPWYPHRANLGLSEGERRVERAAR
                     ETVREASERFGLTVETRPFFEGVSDLSYCGFQGEAGEMATFAANMPGWKRLYSLPTEA
                     LAELDIPILNFGPLGKDAHKNTERLHLPYFMEVFPKLLRSLVRRVAEDGER"
     gene            complement(2727..3932)
                     /locus_tag="PK442_00340"
     CDS             complement(2727..3932)
                     /locus_tag="PK442_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOO86128.1"
                     /translation="MNMEYLKDKPVAVLGGGATARGHAACTALAGREVRLYELPEFFE
                     GLGCIASKREIRVSGRQESLYGFKREGLAKIDVVTDDMAKAVRGAGIIVVSFPAVGYA
                     AFLERLIPHLEDGMVVHFTTANFGSLLFRKMLREAGCTKKIVVGEWSSQPYGIRIKNM
                     GGQQMPEVSVNYWAITLRGAALPMTDQDAFFESKRYLPSLDSVEHPVHGDTVADVGFS
                     NVNPILHCPGSILGVGAMENWGVVYGENKYDFSIYSHAYCPSISKVQLALYREECAIA
                     EAMGVGIQDFPEKAFFSRSNILGSEHMGEKFVVPFEEQYTLAMGTGPFSIYNRYITED
                     IPVGCHIYLELAKKFGIEVPVIESMITLASVMTGTDFRAAGVTLEDLGIAHMNKEALN
                     AWLREGIYA"
     gene            complement(3945..5249)
                     /locus_tag="PK442_00345"
     CDS             complement(3945..5249)
                     /locus_tag="PK442_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO86129.1"
                     /translation="MAITIALLLIIVTLLLGLPVPFCFMLSTTFMVITHAYDPSFLLP
                     YAFSQMSSIVLLAIPLFIMVGGMMDRGGIGTSLVNFVDVFVGRVKGGLGVVAVVSCAV
                     FGSISGSCAATLSCIGSIMFPRLRENGYPMGHSAALVSCAAPLGLLIPPSSQMILFAW
                     VGQQSVLACFLATVGPGIVLMILLSLLNIIMLRNDPNVKVTPRLPFPETMQLLGNRGK
                     KALPALFMPFIVLGGIYGGIMTPTEAAAVAVLYSVPVGFWIYKGLNRNNFMEVIIETA
                     TTTGVVMLMMYCIMMLSRIYVQERLPAMIISSLTSLSSNKYVLMLMINLFMIIIGMIM
                     DDVSAMLLCTPILLPVVVSLGIHPIHFAAIIGTNLGLGNVTPPTAPTLYFGGRMAKTP
                     VSQMMKPAMIFILFAWLPTLLAVTFLPELALWLPRLVLGGRL"
     gene            complement(5340..5864)
                     /locus_tag="PK442_00350"
     CDS             complement(5340..5864)
                     /locus_tag="PK442_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO86130.1"
                     /translation="MSFVETVRSSLVWKFLLKLQKALLVLSSCFVVLIMCVAVLLRYV
                     FKTDLFGIEEIVVIAAFWLYFIGSSYGVYDKSHVKADIIPQMLSARAQAFLSVMVNLT
                     IAALCILFTVWAVDMVWYSYVWMPRTTGLRIPVFISQTSVLLGYALMSLYSVVYFFDD
                     LFSFISMKKEGVSR"
     gene            complement(5919..6938)
                     /gene="dctP"
                     /locus_tag="PK442_00355"
     CDS             complement(5919..6938)
                     /gene="dctP"
                     /locus_tag="PK442_00355"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO86131.1"
                     /translation="MIRKSTACGVLGAFVLGLLLTGVFALPSAAADKLVLKVAGISSP
                     EYRGSKSLEAIKEIVEKGTEGRVVLDVFPANQLGDYTQVFEEIRRGTIEMGLIFLPSQ
                     FDPMLEVGSLPFLASTGEGMQKQLSPGSFVYSIIEESLEKLGVHLLRIYGDGFVGVGL
                     AKQPKNHAKAGVDKDAMIRVAPLAVYKETAEDLGFRTTSIPYADTYSGIQTGVCDGWI
                     GGSSQINYLSFRDVIKYYIPYNCLFDQTAYIVGKQVWEKISPEDRKVIVDAVNEQADK
                     SFADSNAEDIEYQKKLEEAGVEILPITPEEMAAIAEHVRATTWPKLEETYGKETIEKI
                     RESLK"
     gene            complement(7028..7780)
                     /locus_tag="PK442_00360"
     CDS             complement(7028..7780)
                     /locus_tag="PK442_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOO86132.1"
                     /translation="MSSLEKALLLLKRLGEPPYELGLSEIAVHAGIGKSGAYKLLRTM
                     QEQNFVIQDHSSKKYHLSPIMLRLGNVYGHFKGIGETAPPILAHTSKTVGETTYITIW
                     EGDRAFPAYKHRVPGGVYDVNDFIGKSIPLNSGGSAMLLCAYQDPEHIRSLLDASELE
                     RRTPYTLVDRDVLMEEYRKIRDQGYALEDEVFNIGVLSLAVPVFDRERAVRISLAMAA
                     PKKHATEGKIVEWLHLLKDGAEELSYKLQFRR"
     gene            complement(8247..>8931)
                     /locus_tag="PK442_00365"
     CDS             complement(8247..>8931)
                     /locus_tag="PK442_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO86133.1"
                     /translation="KAAERLSGYCSEEALSPDSRVLEKLFPEELYREYVLEEMKKAAH
                     RPEGVPGELETEVVVNGGRTRTIAWTCSRWKSEEENEEGLIAAGRDLTEMRALEGALR
                     ESERRYSSIFLNSNMVMFIVDPEECRILDCNDAACRFYGYTREELTGMSSFDINMTPP
                     EEFKRHLLRIRNGERSSFVFHHRRKDGALRKVDVQASPVLYDGKALVFSLVREMAEGE
                     QKHEDGFSR"
BASE COUNT         1816 a         2851 c         2543 g         1721 t
ORIGIN      
        1 ctcgcccttg ctcgtgctcg atccggaggc cgaccagttc accacatccg cggagagcat
       61 cttctccgcc agctcgaagg agaccctggt ccgcgtcgag ttctcgaaga agcagttcac
      121 caccatcttg ccgcgcagcg cggggacctt cttgatcgga cggttcatca gctcttccat
      181 atagtccgcc tgctccagga agaagtgcag ctcctccctc gtccagcctt cgaggctgat
      241 gaggtcctta tgcctccact ccatgctccg cacctccgtt ccgttcgcag atgacaacgc
      301 cgtccgtgcc gtcgagctcc ttcacccgca cctcgacgtt ctccgagcgc gacgtgggga
      361 tcgtctttcc gacgtagtcc gcggctatgg gcagctccct gtgcccccgg tccaccagca
      421 cggcgagctg cacgcgctcc ggacgcccca ggtcggtgat cgcgtcgagc gccgcccgga
      481 tggtccgccc cgtgaagagg acgtcgtcca ccagcaccac gcgcttcccc gtgatgtcgc
      541 cggggagcga ggtgctgtgc accacgggct ggtcggcaag cgtcgagatg tcgtcgcggt
      601 agagcgtgat atccagctcg cccgcaggga ctttcacctt ctcttcggcc ttgaagatct
      661 ccctcaagcg ggccgcaaga tgcacgccgc gccgctggat tcccagaagc accacgtcgt
      721 cgagcccctt gttccgctcg acgatctcga cggcgatgcg ccggagcacc cgctccatat
      781 ccctctccgt catgaccgtc gctttttccg tccacacatt tccggactgc gctttcacca
      841 cgatcccatc tcccttccgt aaaaaaagag cccctctccc gacgggagaa aggctccgac
      901 attgtgtccg agaacaggaa gagaagcgct gcgaacacgc ggccgacaga tagtactcca
      961 catggcgcct cccccctctc ttcaagctgg gggaatgata ccacactctc ataatctatg
     1021 caagcggttg cagagaaaaa atcgaggaag aaagttacct ttctccatct tccgcaactc
     1081 tcctgacgag agaacgcagg agcttcggaa agacctccat gaagtacgga aggtgcagac
     1141 gctccgtatt cttatgggcg tccttcccga gcgggccgaa gttcaagatc gggatgtcca
     1201 gctccgcaag cgcctccgtc ggcaggctgt agagccgttt ccagccgggc atgttggccg
     1261 caaacgtcgc catctccccc gcctcgccct gaaaaccgca gtagctgagg tcggaaaccc
     1321 cctcgaagaa cgggcgcgtt tccaccgtca gcccgaaccg ctcgctggcc tcccgcaccg
     1381 tttcgcgcgc cgcacgctct accctgcgct ccccttctga aaggccgagg ttcgcgcggt
     1441 gagggtacca cggcggcagg aagccgaaga cgacaagcgg cccaggcagc gcgcacgcct
     1501 cgaccatgct ttcgacgagc gcgacggcct tcgtccgttc gtcggccccg gaaggcgtcc
     1561 gggaaagaac ctcttcgacg aagccgggga accccgcgcc cagttttgcc tccgccatgc
     1621 ggcgcagctc ttcgtaggag agggccatgg aattccactt tgcgtcggag cgtcccgccc
     1681 cgttcatctt cgcgaacgat tcggcgttgc gctcgtgcgc ctctatcgct tcccgcagcg
     1741 ctttctccgc aatggcgcgc atttcgccga ggatgcgccc cggcagcttc gtcgccgtga
     1801 ggtagctgta gaaggagaag gcgcgcgtca taatcgtcgc cgagtactcc ctccgaaggt
     1861 ccgtctggcg catacagccg aacggggggt acgctctccc gccgagcgaa tccgcatact
     1921 cggggttgcc ttcaagctcc aggttgatcc gggagatcac cggcgcggcg ccgaacccct
     1981 cgtagtactc cccgacgtgc gtctcctttc cggcgcagaa gaaaaaggtg ttgatcttcc
     2041 cgatgctgcc aaggtagata gacggaagcg cccgctcccc ccctgcaaac gtgggttcga
     2101 gttcgatgca cgcgcggtag cgcagcccct cttccttttg cctgcgcgcc agctgcgccg
     2161 atgcggcgag catccccgcg gagttgttct cctcgtcggg aaccgcgagc gcgagcagat
     2221 tcgcgccgag acttccggga tccgacgccg cctccgccag gagttcgata cccgccgcaa
     2281 gtccgctctt catatcggcg acgccccgcc cgaagagcca ctccccgctt tcgaggtcgc
     2341 gacgggcgtc ctcgggaagc gcaacgtcct ccagacgccg cgtgtactcc tccgggtcga
     2401 acgcgaaggg ggcaagctcg ccgcacgcct ctgcgctcac cacatccgcg tgccccgcca
     2461 gaatgaccgt ctccgccgtc ggccccggcc tgcgtacgaa tgcgaaaagg aaatttcgtc
     2521 cgaacgggtc gtcctcaagc ggaaggaggc ggagatcctc agggtgctcc cgaaagtacg
     2581 gaagcacggc gagcgtgtcg tacagcaagc gcgtcacgcg gttttcatgc tccggcgagg
     2641 gggagacgct cggaatccgc agcatgtcga gcagcagacg gcagagcccg ctctcggcgt
     2701 attttttcag aacgctcccc ctctcctcag gcatagattc cctcccgaag ccaggcgttc
     2761 agcgcttctt tgttcatgtg ggcgattccc aggtcctcca gcgtcacgcc ggcggcccgg
     2821 aagtccgtcc cggtcatgac ggaggcgagc gtgatcatgg actcgatgac gggaacctcg
     2881 attccgaact ttttcgccag ctccagatag atgtgacagc cgacgggaat atcctcggtg
     2941 atgtagcggt tgtagatgga gaacggcccc gttcccatcg ccagcgtgta ctgctcctcg
     3001 aaggggacga cgaacttctc ccccatgtgt tccgacccca gaatgttcga cctcgaaaag
     3061 aaggccttct ccgggaagtc ctgaatgccg acacccatcg cttcggcgat ggcgcactct
     3121 tcgcggtaga gggcgagctg caccttcgag atcgaagggc agtatgcgtg cgagtaaatg
     3181 gagaaatcgt acttgttctc accgtagacg acgccccagt tctccatcgc gccgacgccg
     3241 aggatggacc ccgggcagtg caggataggg ttgacgttgc tgaaaccgac gtccgcgacg
     3301 gtatccccat gaacggggtg ctccacggag tcgagcgacg ggaggtagcg cttcgattcg
     3361 aagaaggcat cctggtccgt catcggcagc gccgccccgc gcagcgtgat cgcccaatag
     3421 ttgacgctca cctcgggcat ctgctggcct cccatgttct tgatccgaat gccgtagggc
     3481 tgactgctcc actcgccgac aacgatcttc ttcgtgcatc cggcttcgcg gagcatcttc
     3541 cggaagagca gcgagccgaa gttcgccgtc gtgaaatgca cgaccattcc gtcctcaagg
     3601 tgcgggatca gacgctcaag aaaggctgcg taccccacgg cggggaagct gacaacgata
     3661 atccccgcgc cgcggacggc cttcgccata tcgtcggtga ccacgtcgat cttcgcgagt
     3721 ccctcgcgct tgaatccgta caggctctcc tgccttccgc tgactcgtat ttcgcgtttg
     3781 ctcgcgatac accccagccc ctcgaagaac tcaggcagtt cgtaaaggcg gacctcccgc
     3841 ccggcgagcg ccgtacaggc ggcatgaccg cgcgccgtcg ccccgccgcc gagaaccgct
     3901 accggtttgt ctttcaaata ctccatattc atatcggacg cctcctagag ccggccgccg
     3961 agcacaagac gaggcagcca gagtgcaagt tccggcagga aggtcaccgc cagaagcgtc
     4021 ggcagccatg cgaaaagaat gaagatcatg gccggtttca tcatctgcga aacgggcgtc
     4081 ttcgccatgc gcccgccgaa gtagagcgtc ggcgcagtcg gcggcgtcac gtttcccagg
     4141 ccgagattcg tcccgatgat cgcggcgaaa tggatggggt gaatccccag ggagaccacg
     4201 accggaagca gtatcggggt gcagagcagc atcgcgctca catcgtccat gatcatgccg
     4261 atgatgatca tgaagaggtt gatcatcagc atcaacacgt acttgttgct cgacagggag
     4321 gtaagagagg aaataatcat ggccggaagc cgttcctgga catagatgcg gctgagcatc
     4381 atgatgcagt acatcatcag catcacgacg ccggtggtcg tcgccgtctc gatgatcacc
     4441 tccatgaagt tgttccggtt gagccccttg tagatccaga accccacagg cacggagtag
     4501 agcacggcaa ccgcggcagc ctccgtcggc gtcatgatgc ctccgtagat tcctccgagg
     4561 acgatgaacg gcatgaagag cgcggggagc gccttctttc cgcggttccc cagaagctgc
     4621 atcgtctctg ggaagggtaa acggggagtt actttcacgt tcgggtcgtt tcggagcatg
     4681 atgatattca aaaggctgag aagaatcatg agaacgatgc cgggtcccac agtggcaaga
     4741 aagcacgcga gcaccgactg ctggcccacc caggcgaaga gaatcatctg cgaactcggc
     4801 gggatcagca ggccgagcgg agcggcgcat gaaacgagcg cggcgctgtg ccccatcggg
     4861 tagccgtttt ccctgagacg gggaaacatg atcgagccga tgcacgagag cgtcgcggcg
     4921 cagctccccg aaatggatcc gaagacggcg caggagacca ccgccactac gccgagcccc
     4981 cccttgacgc ggccgacgaa gacgtcgacg aagttgacga gcgacgttcc tatacctccc
     5041 ctgtccatca tcccgcccac catgatgaag agcggaatgg ccagcagcac gatggagctc
     5101 atctggctga aggcgtacgg gagcaagaag gacggatcgt acgcatgggt aatgaccatg
     5161 aaggtcgtgg agagcatgaa gcagaagggg acgggcagtc cgagaagaag cgtcacgatt
     5221 atgagcagaa gcgcaatggt tatcgccatt cgtgtttcct ccagtgcgcc ggccgtccgc
     5281 tccgccggaa cgcggcgcgc tctcggggaa atgcgtggac cggcagtaag atggactccc
     5341 tagcgggaga ctccctcctt cttcatcgaa atgaaggaga atagatcgtc gaaaaaatag
     5401 acgacggaat agagagacat aagcgcatac cctaggagca ccgatgtctg cgagataaag
     5461 acaggtattc tcaaaccggt cgtgcgcggc atccagacat agctgtacca caccatgtcg
     5521 acggcccaca cagtgaagag aatacagagc gcggcgatcg tcaggttgac catcacgctt
     5581 aaaaaggcct gggcacgcgc agagagcatc tgcggaatga tgtccgcctt gacgtggctc
     5641 ttgtcgtaca cgccgtacga acttcctatg aaatacagcc agaaggccgc aatcaccacg
     5701 atctcctcta tcccgaacag atcggtcttg aatacgtacc gcagcagcac cgcaacgcac
     5761 atgatcagta ccacgaagca gctgctcagg acgagaagcg ctttttgcag tttgaggaga
     5821 aacttccaga ccaaagacga tctgacggtc tcgacgaaac tcacggcaac cccctccagt
     5881 actgtgcggg gcgtcgccgc cccgcacgca gagtctttct acttcaacga ttcccggatc
     5941 ttctcaatgg tttccttgcc gtaggtctcc tcaagcttag gccacgtcgt cgcgcgaaca
     6001 tgctcggcaa tcgccgccat ctcctcggga gtgatcggca gaatctccac tcctgcctcc
     6061 tcaagcttct tctggtactc gatgtcctcc gcgttgctgt ccgcaaagct cttgtcggcc
     6121 tgctcgttca cggcgtcgac gatcaccttg cggtcctcgg gggagatctt ctcccaaacc
     6181 tgcttgccga cgatgtacgc cgtctggtcg aagaggcagt tatagggaat gtagtatttg
     6241 atgacgtcgc ggaagctcag gtagttgatc tgggaggaac cgccgatcca gccgtcgcaa
     6301 acgcccgtct ggatgccgct gtacgtgtcc gcgtagggga tggaggtcgt ccggaagccg
     6361 aggtcctcgg ccgtctcctt gtagacggca agcggcgcca cgcggatcat ggcgtccttg
     6421 tccacacctg ccttcgcgtg gtttttcggc tgcttggcaa gaccgacgcc gacgaacccg
     6481 tcgccgtaga tccggaggag gtgcacgccc agtttttcaa gcgattcctc tatgatcgaa
     6541 tacacgaagg accccgggga gagctgcttc tgcatcccct cgcccgtgga ggcaaggaag
     6601 gggagcgagc cgacttcgag catggggtcg aactggctgg ggaggaagat gagccccatc
     6661 tcgatcgtgc cgcgccggat ctcctcgaaa acctgcgtat agtcgccgag ctggttcgcg
     6721 gggaagacgt ccagaaccac ccgtccctcc gtccccttct cgacaatctc cttgatggct
     6781 tcgagcgact tgcttccgcg gtactccgga gaggagatcc cagccacctt cagcacaagt
     6841 ttgtccgccg cggcggacgg cagcgcgaaa acccccgtca gcagcaaccc gagaacaaac
     6901 gctcccagaa ctccgcacgc agtacttttc cttatcatta tgacatcgct cctttcagat
     6961 tctttagtga cttgtacaga ccttttccgc gcagtcattt ttccccgcaa ctcctccttt
     7021 cacggcgtca acgcctgaac tggagcttgt acgaaagttc ctcggcgccg tctttcagaa
     7081 gatgaagcca ttcgacgatt ttcccctccg tcgcgtgctt cttcggcgca gccatggcga
     7141 gagagatgcg aaccgctctc tccctgtcga aaaccggaac tgcgagtgaa agcaccccga
     7201 tattgaagac ctcgtcctcc agggcatacc cctgatcgcg aatcttccgg tactcctcca
     7261 tcaaaacgtc tctgtcaaca agcgtgtagg gtgtgcggcg ctccagctcc gaagcgtcaa
     7321 gaaggctgcg gatatgctcc ggatcctggt aggcgcacag cagcatcgcg gaccctccgg
     7381 agttcagcgg aatactcttg ccgatgaaat cgttcacgtc gtacacgcct ccgggtaccc
     7441 tgtgtttgta ggcggggaag gcgcggtcgc cctcccagat cgtgatatag gttgtctcgc
     7501 cgacggtttt ggacgtatgc gcaagtatcg gaggggccgt ctcgccgatt cccttgaagt
     7561 gaccgtacac gttcccgagg cgaagcatga tggggctgag atggtatttt ttggaggagt
     7621 gatcctgaat gacgaagttc tgctcctgca tcgtccggag cagcttatag gcgccgctct
     7681 tgccgatgcc ggcatggacg gcgatctcgg aaagaccgag ctcgtagggc ggttcgccga
     7741 gacgtttcag aagaagcaag gctttttcca gactgctcac gcgtacacct cgtttcctta
     7801 caggaaatga agtttcccat agaagaattt actgcactat atcagctact cagtgatttg
     7861 tcaaaaaatc ttttcttccg ctctttcccg gcgcactcgc cccacgtacg cttcagcaga
     7921 aaaactgccg ctacaaatta acagagagca aacgaaccgc ctcggacacg cgtccgaaac
     7981 ggttcgttag gcagttctga aagagaaaag ggaaaacggg tacgaaaatg ctaagaacaa
     8041 gaaaggagga cgctgtaacg tctcatcaaa ggctgttgga tactcttcgt tcaactcgcc
     8101 ggcctccgcc ggagagcgga tacaaacatc tggactgtcg ccgctggcaa aactgcccgg
     8161 cggagagttt ctttttcggt atctcggtcg aagaacgacg tcgttcagcc ggatctcaag
     8221 agaggagggg aagcgcgctc cgtccgctac cgcgagaaac catcttcatg cttctgttcg
     8281 ccctcggcca tctcccgcac aagagaaaaa acaagcgctt tgccgtcgta gagcaccggg
     8341 gaagcctgta cgtcaacctt ccgcagcgcc ccgtccttgc gccggtggtg gaagacgaag
     8401 ctgctccgct cgccgttgcg tatccgcaac aaatgccgtt tgaactcctc cggcggggtc
     8461 atgttgatat cgaacgagct catgcctgtc agctcctctc tggtgtagcc gtagaagcgg
     8521 caggcggcgt cgttgcagtc cagaattctg cactcctccg gatcgacgat gaacatcacc
     8581 atgttcgagt tgaggaagat cgacgaatag cgccgctcgc tctccctgag ggcgccttcc
     8641 agcgcgcgca tctccgtcag atccctcccg gcggcgatga ggccttcttc attctcctct
     8701 tcgctcttcc accggctgca cgtccatgct atcgtgcggg tccggccgcc gttcaccacg
     8761 acctcggtct ccagctcacc gggaaccccc tccggccgat gcgcggcctt cttcatctct
     8821 tcaagcacgt actcacggta cagctcttcc gggaagagct tctccaaaac acgggaatcg
     8881 ggagacagag cctcttcgct gcagtagccg ctcagccgtt cggccgcctt g
//
LOCUS       DAOSWW010000009         8580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_5262_length_8580_cov_3.465103, whole genome shotgun sequence.
ACCESSION   DAOSWW010000009 DAOSWW010000000
VERSION     DAOSWW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5262_length_8580_cov_3.465103"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PK442_00370"
     CDS             <1..1194
                     /locus_tag="PK442_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO86134.1"
                     /translation="SLAGRDILHGWGKFVLTSVGLGLLIGVTLTMGGVYRGMVDDAKV
                     LLDNSGADLWVVQRETLGPYAESSSIYDDVRWSVFGMPGVERAANVTYLTTQVRRGGE
                     DTRVMVAGVEPGEPGAPGQPGYLTAGRHLARSHYEAVADEASGFRLGDTIRIRRNSYT
                     VVGLTRRMVSSGGDPMVFIPLKDAQEAQFLKDNDAILQQRRRTAANPSLNRPGVPGML
                     DGVIASQGVNPYVNAVLVRLLPGADPEKTAEHMRRWMRLEVYTRAQMEEILLGKLIAT
                     SSKQIAMLLVILAVVSAAIVAFIIYTLTLGKIREIAVLKLIGTKNRTIIGMIMQQAIA
                     LGVLGFMIGKVSATFWAPKFPKYVLLEVRDAATGFAAVIIMCVIASLLAIRVAVKVDP
                     AEAIG"
     gene            1204..1923
                     /locus_tag="PK442_00375"
     CDS             1204..1923
                     /locus_tag="PK442_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO86135.1"
                     /translation="MSGIRIEALRKRYGSGETAVDALKGVDMEVAPGEVVGLIGPSGS
                     GKSTLLKCLGAVIEPTGGKMVLGGKTIYDDGWKISDLRMLRRDSIGFIFQAPYLIPFL
                     NALDNVALLPMLAGHPDDESRERAMELLEALDVAHRAAAMPSQLSGGEQQRVSIARAL
                     ANHPPVILADEPTAPLDSERALSVVRILHSMAHQYETAIIVVTHDEKIIPTFRRIYRI
                     RDGKTVEEAGEGRSTAEEITE"
     gene            1939..2286
                     /locus_tag="PK442_00380"
     CDS             1939..2286
                     /locus_tag="PK442_00380"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO86136.1"
                     /translation="MIITRKYEEQKIAETAHRVDARGMYETEDAAISIIALQPGESLK
                     RHITPVDVAFFVLDGKGTVEIGDEKQEVGKYTLVESPKDILHCWYNTGDELLRFMVIK
                     APKPTKKTIFVGA"
     gene            2544..3032
                     /locus_tag="PK442_00385"
     CDS             2544..3032
                     /locus_tag="PK442_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOO86137.1"
                     /translation="MTFALSAADVFEAAILLEERGARFYADAAARCTGEAKKLLSGLS
                     SMEKNHAERFRSILEELRTSSSAPEPRPEEAAQYLEALTSDRIFTETISIGAKESYPE
                     ILEKAIVVEKNSVFFYTAVKDILSSKMEAKDVDRLIAEEIGHFHSLTDALRIRRERNG
                     GA"
     gene            3035..4057
                     /gene="asrA"
                     /locus_tag="PK442_00390"
     CDS             3035..4057
                     /gene="asrA"
                     /locus_tag="PK442_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HOO86138.1"
                     /translation="MSSFSFSLEEFSRVFDALGELYALSGPKRFPGKGRFTGTDLVKY
                     APLVSFDELELDEKSSMSPKSLVFPPDETTFYFNGDEFTEAPLEDERETVVFLRPCDI
                     NGFRRLDAIFLENGGTKDPYYLRRREKLHFFMIECATSFENCFCVSMGSNVAEEYAVA
                     LRFGQDSVLVQVKDERFAGLFASGAPSEFTPEFVRENARTVEVPDVDEMPPELFLHPL
                     WDEYDARCIACGRCNTSCVTCTCFSTRDIFYADSRRSGERRRVWDGCHLDGFTDMAGG
                     HSFRKKKGERMRFKTFHKIYDFKKRFGYNMCVGCGRCDDVCPEYISFAECINKVAAAL
                     KGGDAK"
     gene            4054..4848
                     /gene="asrB"
                     /locus_tag="PK442_00395"
     CDS             4054..4848
                     /gene="asrB"
                     /locus_tag="PK442_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HOO86139.1"
                     /translation="MKENPYRPFPAKITGIKAETDCEFTLTVAASVAMTPGQFFQVSL
                     PKIGEAPISISGFGPDWIQFTVRAVGRLTNALQELRTGENLFIRGPYGTGFPLDTFRG
                     SNLAIVAGGSGTAPVRPLIRGALDGSLPVRSLRVIAGFKSKESLLFANEFDEWRTKGE
                     VLVTIDKPQEGWDGPTGLVTEHIRAMKLDDPADVQFVVVGPPVMMKFAVAEVLLLGVP
                     EDNIWVSFERLMSCGLGKCGHCKIDSAYICLDGPVFRFSRAAGLID"
     gene            4862..5866
                     /gene="asrC"
                     /locus_tag="PK442_00400"
     CDS             4862..5866
                     /gene="asrC"
                     /locus_tag="PK442_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HOO86140.1"
                     /translation="MTLDLNRKKVTKNAFRITKVRGATAIRVRVPGGHLDAELMKTIA
                     QIAERFGDGTLHITIRQGFEIPNIPFERMPEVNAALAPIIERLELPHGVKIESPSDGY
                     PAAGMRNISACIGARVCPFANTDTTALAQKLEALVYPNDFHVKIAVTGCPNDCVKAHM
                     QDFGIIATCRPEYDYDQCIGCEACVDACRQKVTGALRMQDGKVVKDDRRCIGCGECVL
                     ACPVAAWTRPPVTLYRLLIMGRTGKKNPRLAMPFLDRVTEEVVLKVIKNTLDYVDKHI
                     LRSLVKEHIGYIVDRTGYPVFRDAVLEGVQLNPEARIAKTMHWPGYYTLDDGNMKQAE
                     "
     gene            5932..6156
                     /locus_tag="PK442_00405"
     CDS             5932..6156
                     /locus_tag="PK442_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86141.1"
                     /translation="MLLFLFSPEAGGAGKTGGLFAAQRSQSGEKALLRRAEDVETFPR
                     EDVRKGEPDGGERCTSSPSGLCTATNRSSA"
     gene            complement(6392..6721)
                     /locus_tag="PK442_00410"
     CDS             complement(6392..6721)
                     /locus_tag="PK442_00410"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOO86142.1"
                     /translation="MKSDAPTEALRRIQECVKRRDILWTYHVFMRMQGRCIPRSSILD
                     SVASYEIIESYPEDKYMASYLVRAECGADVVHVLFAFDIADKFVRIITTYRPDAALWS
                     EDGRKRR"
     gene            complement(6832..7053)
                     /locus_tag="PK442_00415"
     CDS             complement(6832..7053)
                     /locus_tag="PK442_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOO86143.1"
                     /translation="MKCHVCGAGMQEMKTDLPFKINERSIIVVRDVPVIQCSGCREYM
                     LSDEVMERLDELLAGVNSNADLEIVRFAA"
     gene            7485..7766
                     /locus_tag="PK442_00420"
     CDS             7485..7766
                     /locus_tag="PK442_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86144.1"
                     /translation="MDIVTKIRNLCSSEVDWVKEAGKAFADSADDLFDLFAERKAPSP
                     KFKVRLARTFSDVNSALAACGMEKIEGESEREIAAAYFAYCKGVIESRM"
     gene            7877..8161
                     /locus_tag="PK442_00425"
     CDS             7877..8161
                     /locus_tag="PK442_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOO86145.1"
                     /translation="MGARVTKTKRPGFRSVNVRLVARDKELAENIFRKVGLTPTEAIR
                     LFYRQTINTGGIPFGLVLPEETLAAMREAESGTAEEISLDDLKAELDAIC"
     gene            8148..8438
                     /locus_tag="PK442_00430"
     CDS             8148..8438
                     /locus_tag="PK442_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HOO86146.1"
                     /translation="MRSVRQTRQTAAFKRDFKREIKRASRHILKERLWGVVAALTMGS
                     PLDPSYRDHPLGGNWRGHRECHIAPDLLLLYYLSEDALTLVRLGSHAELFGL"
BASE COUNT         1787 a         2479 c         2673 g         1641 t
ORIGIN      
        1 agcctggccg ggagggatat cctccacgga tgggggaagt tcgtccttac cagcgtcgga
       61 ctggggctgc tcatcggcgt gacgctcacc atgggcgggg tgtaccgcgg catggtggac
      121 gacgcgaagg tgctgctcga caacagcggc gcagacctgt gggtggtgca gcgtgagacg
      181 ctcggcccct acgccgagtc gtcgagcatc tacgacgacg tccgctggag cgtcttcggg
      241 atgccgggcg tggagcgcgc cgcgaatgtc acctacctta ccactcaggt acggcgtgga
      301 ggcgaggata cccgggtgat ggtcgcgggg gtcgagccgg gggaacccgg cgctccgggg
      361 cagccggggt accttacggc ggggagacat ctcgcccgca gtcactacga ggccgtggcc
      421 gacgaggcgt cgggcttccg tctcggggat acgatccgca tccgcaggaa cagctacacc
      481 gtggtcgggc tcacgcggcg gatggtctcg tccggaggcg acccgatggt cttcatcccg
      541 ctgaaagacg cgcaggaggc gcagttcctc aaggacaacg acgctattct gcagcagcgg
      601 cggaggacgg cggcgaatcc gtcgctcaac cggccggggg ttcccggaat gctcgacggc
      661 gtgatcgcat ctcagggcgt caacccgtat gtcaacgccg ttctggtgcg cctccttccg
      721 ggcgccgatc ccgagaaaac ggcggaacac atgcggcggt ggatgcgcct cgaagtctac
      781 accagggcgc agatggaaga gattctgctg gggaaactga tcgccacgtc gtcgaagcag
      841 atcgctatgc ttcttgtgat actcgccgtt gtgagcgccg ccatcgtcgc cttcatcatc
      901 tatacgctca ccctggggaa gatcagggag atcgcggtac tcaaactcat cggcacgaag
      961 aaccggacga ttatcggcat gatcatgcag caggctatcg ccctcggcgt cctcggcttc
     1021 atgatcggca aggtctcggc gaccttctgg gcgccgaagt tcccgaaata cgtgctgctg
     1081 gaggtccgcg acgcggcgac gggattcgcc gccgtgatca taatgtgcgt gatcgcaagt
     1141 cttctcgcga tacgggtcgc cgtcaaggtg gaccctgcgg aagcgatagg gtaggcggcc
     1201 cctatgagcg ggatacgcat cgaagcgctc aggaagcggt acggctcggg agagacggcg
     1261 gtggacgcgc tcaagggagt ggacatggag gttgctcccg gagaggttgt cgggctcatc
     1321 gggccttcgg gatcggggaa gagtacgctg cttaagtgtc tcggcgccgt gatcgaaccg
     1381 acgggaggaa aaatggtgct cggcgggaag acgatctacg acgacggatg gaagatctcc
     1441 gacctccgga tgctgcggcg ggacagtata gggtttatct ttcaggcgcc ctaccttatc
     1501 ccttttctca atgcgctgga caacgtcgcg ctgctcccga tgctcgccgg tcatcccgac
     1561 gacgaatcgc gggagcgggc catggagctt ctggaggcgc tcgacgtcgc gcaccgggcg
     1621 gcggccatgc cgtcgcaact ttccggagga gagcagcagc gcgtctcgat cgcccgggcg
     1681 ctggcgaatc atccgccggt catactggcc gacgagccca ccgcccctct ggacagcgag
     1741 cgcgctctct ccgtggtccg gattctgcac tcgatggcgc accagtacga gacggcgatc
     1801 attgtagtga cgcacgacga gaagattatt ccgactttcc gcaggatcta caggatccgc
     1861 gacggaaaga cggtggaaga ggccggcgag ggccggtcga cggcagaaga aataacagag
     1921 tgaaggagga agaatactat gattatcaca aggaaatatg aagagcagaa gatagcggag
     1981 accgcgcaca gggtggacgc gcggggcatg tacgagaccg aggacgcggc gatttcaatc
     2041 atcgcgctgc aaccggggga gtcgttgaag cggcacatca ccccggtgga cgtcgcgttt
     2101 ttcgtcctcg acgggaaggg cacggtcgag atcggcgacg agaagcagga agtgggaaag
     2161 tacacgctcg tcgaaagccc gaaggatatt ttgcactgct ggtacaacac cggcgatgaa
     2221 ctgctgcggt tcatggtgat caaagcgccg aaaccgacca aaaaaacaat cttcgtcggc
     2281 gcctgacgga gaggctgttc cggaagcgag cgcgccgcgt ccttggggaa cggcgcgttc
     2341 gctgtataat aaaagcagga acgggagttc caccgtttcc gatcaagatg gaggcgtctt
     2401 gacatattca ggaattaaag taccataata cctgtaaaga gtggagcgaa agtgtgcaag
     2461 cgtcttaaat accggcggtg tagtacggtg gtccgctgca cactccgccg gagagagaac
     2521 cggagacgaa aggggagatt gttgtgactt ttgcgctttc agcggcggat gtcttcgagg
     2581 ccgcgatcct gctggaggag agaggagcgc gcttctacgc cgatgcggct gcacgctgta
     2641 ccggcgaggc gaagaagctg ctctcggggt tgtcgtccat ggagaagaac catgcggaac
     2701 gcttccggag tatcctggag gagctccgca cgtcgtcttc cgctccggag ccccggccgg
     2761 aagaggcagc acagtatctt gaggcgctga cctccgacag gatattcaca gaaacgatca
     2821 gcatcggggc aaaagagagc taccctgaga tcctcgaaaa agcgatcgtc gtcgagaaga
     2881 actccgtctt cttctacacc gcggtgaagg atatcctctc gtccaagatg gaggcgaagg
     2941 acgtcgaccg cctcatcgcc gaggagatag ggcacttcca ttcgctcacg gacgcgctcc
     3001 gcatccggcg ggagcggaac gggggggcgt agcgatgtcc tccttctcct tctcgctgga
     3061 ggagttctcc cgcgttttcg acgcgctggg cgagctgtac gccctctccg ggccgaagcg
     3121 ttttcccggc aaggggcgct ttaccggcac ggacctggtg aaatacgctc cgctcgtctc
     3181 tttcgacgag ctggaactgg acgagaagag tagcatgtcg cccaagtcgc tcgtcttccc
     3241 gcccgacgag acgacgttct acttcaacgg cgacgaattt accgaggctc cgctcgagga
     3301 cgagcgggag accgtcgtct ttctgcgtcc ctgcgatatc aacggcttcc ggaggctcga
     3361 cgccatcttc ctggagaacg gcggaaccaa ggacccctac tacctgcgcc gcagggagaa
     3421 gcttcatttc ttcatgatcg aatgcgcaac ctcgttcgag aactgcttct gcgtctccat
     3481 ggggtccaac gtcgccgagg agtacgccgt cgctctgcgt ttcggacagg attcggtcct
     3541 cgtccaggtg aaggacgagc gcttcgccgg tctcttcgcc tccggggcgc cttccgaatt
     3601 tacacccgag ttcgtccgtg agaacgcacg aaccgtcgaa gtcccggacg tcgacgaaat
     3661 gcccccggaa ctcttcctgc acccgctgtg ggacgagtac gacgcgcgct gcatcgcctg
     3721 cggcaggtgc aacacctcct gcgtcacctg cacctgcttt tccacgcggg acatcttcta
     3781 cgcggacagc cgccgctccg gcgagcgccg ccgcgtctgg gacggctgcc atctcgatgg
     3841 gttcaccgac atggccgggg ggcactcctt caggaagaag aagggggagc ggatgcgctt
     3901 caaaaccttc cataagatat acgacttcaa aaaacgcttc ggctacaaca tgtgcgtcgg
     3961 gtgcggcagg tgcgacgacg tctgtccgga gtacatctcg ttcgccgagt gtatcaacaa
     4021 ggtagccgcc gccctgaaag gaggggacgc gaaatgaagg agaatcccta ccgccccttc
     4081 ccggccaaaa tcaccgggat caaggcggag acggactgcg agttcacgct gacggttgcc
     4141 gcttccgtcg caatgacgcc cggacagttc ttccaggtct ccctgccgaa gatcggcgag
     4201 gcgcccatct cgatcagcgg cttcggaccg gactggatcc agtttactgt ccgcgccgtc
     4261 gggcggctca cgaacgccct gcaggagctt cgtaccggag agaacctctt catcagaggc
     4321 ccctacggaa cgggtttccc gctggatacg ttccgcggga gcaacctggc gatcgtggcc
     4381 ggaggttccg gcacggcgcc ggttcgtccg ctcatccggg gcgcgctcga cggctcgctt
     4441 cccgtgcgct ctctgcgcgt gatcgcggga ttcaagtcga aggagagcct gctcttcgcc
     4501 aacgagttcg acgagtggcg cacgaagggc gaggtcctcg tcacgatcga caaaccgcag
     4561 gaggggtggg acggcccgac ggggctggtc accgagcata tccgcgcgat gaagctcgac
     4621 gaccccgccg acgtgcagtt cgtcgtcgtg gggccgcccg tgatgatgaa gttcgctgtt
     4681 gcggaggtcc tgctgctggg cgtgcctgag gacaatatat gggtctcctt cgagcgcctc
     4741 atgtcatgcg gccttggaaa gtgcgggcac tgcaagatag actccgccta catctgcctg
     4801 gacgggccgg tattccgctt ctcccgagcc gccgggctca tcgactgaga ggtggcgcga
     4861 gatgaccctc gacctcaaca gaaagaaagt cacgaagaac gctttccgca tcacgaaggt
     4921 gcgcggcgca acggcgatcc gcgtccgcgt accgggcgga cacctcgacg ccgaactcat
     4981 gaagaccatc gcgcagatcg cggagcggtt cggggacggc acgctccaca tcaccatccg
     5041 gcagggcttc gagattccga acatcccctt cgaacggatg cccgaggtga acgcggcgct
     5101 cgcgccgatc atcgaacgcc ttgagctgcc gcacggggtg aagatcgagt cgccgtcgga
     5161 cggctacccc gcggcgggga tgcggaacat ctccgcgtgc atcggcgcgc gcgtctgccc
     5221 cttcgccaac accgacacga ctgccctcgc gcagaagctc gaagcgctcg tctaccccaa
     5281 cgacttccac gtcaagatcg ccgttaccgg ctgtcccaac gactgcgtca aggcgcacat
     5341 gcaggacttc ggcatcatcg ccacgtgcag gcccgagtac gactacgacc agtgcatcgg
     5401 ctgcgaggcg tgcgtcgacg cgtgcaggca gaaggtgacg ggagcgctca ggatgcagga
     5461 cggcaaggtg gtcaaggacg accggcggtg catcggctgc ggcgagtgcg tcctggcctg
     5521 ccccgtggcc gcgtggacgc gcccccccgt cacactctac cggctgctca tcatggggcg
     5581 caccggaaag aagaatccgc gcctcgccat gcccttcctg gacagggtga cggaggaggt
     5641 cgtcctcaag gtgatcaaaa acaccctcga ctacgtggac aaacacatcc tgcggagcct
     5701 cgtcaaggag cacatcggct acatcgtgga caggacgggg taccccgtct tccgcgacgc
     5761 ggtgctcgaa ggagtgcaac tgaaccccga ggcgcggatc gccaagacga tgcactggcc
     5821 ggggtactat acgctggacg acgggaatat gaagcaggcg gagtagcggt cggacgattc
     5881 cgcgcggagt ctataaccac acaaaagagg ggagcgccgg ttccggccgg agtgctcctc
     5941 tttttattct ccccggaggc cgggggtgcc ggcaagaccg gcggtctctt cgccgcccag
     6001 cgctcgcaga gcggcgagaa agctctcctc cggagggcag aagatgtcga gacgttcccc
     6061 cgtgaagatg tgcggaaggg cgagccggac ggcggcgagc ggtgcaccag cagcccctcc
     6121 ggcttgtgca ccgcgacgaa ccgctcatcc gcgtagagca ggcggagcgg ctccgcagtc
     6181 atgcgcgctg tcgtcgttgc ttcgttcact ctgccctctc cttccgcagc gtgaataccc
     6241 gataccgccg ggcgatgttc gcgtgcgcgg agatcatcgt accacgcata tcgtcggtaa
     6301 aagcgaacat ttctattcac aggatgtgtt cctgaaacag cggagagata cttgaaaaaa
     6361 gcgcgttcat gctggttaac tttattttaa cttaccgcct cttcctgccg tcttcggacc
     6421 agagggccgc atccggtctg tacgtcgtta taatgcggac gaacttgtcc gcgatatcga
     6481 atgcaaagag aacatgcacg acatccgcgc cgcattcggc tcgtacaaga tagctggcca
     6541 tatacttatc ctccggataa ctttctatta tttcgtaaga agcaacggag tcaagaatgc
     6601 tgcttcgtgg tatgcaacgg ccttgcatac gcataaacac atggtatgtc cagaggatat
     6661 ctctccgttt cacacattct tgaattcgtc gaagcgcttc tgttggagca tcgctcttca
     6721 tttgaaaaac ccttccggaa taagaagaaa agcattttgt gaagcacccc gaagaggttg
     6781 ctttgcccgt gttacgctat aacaggctcc gacattattc aacattccgt ttcaagcagc
     6841 gaaacgaacg atctccaagt cggcgttgga gttcacgccg gcgagcagct cgtcgaggcg
     6901 ctccataacc tcgtcggaaa gcatgtattc ccggcatccg gaacactgaa tcaccgggac
     6961 atcgcgaacg acaatgatgg aacgttcatt gatcttaaaa gggaggtcgg ttttcatctc
     7021 ctgcatcccg gctccgcaaa catgacattt catcgtattt ccctccgtcc attttcgccg
     7081 gcgcaacgtt ctggtcagac atggagcgcc ttctcgaggc gaaagtattc cgttcgtcag
     7141 aaatcagttt tctcatcggc ttgcgaatgg ttgccacaaa attgttatca tattatatca
     7201 tcgagaaaac agcctttcgg atcgaaggag acgcgaatag cgatcccatg cagaacgatt
     7261 cgcgacccta tcgattccga gcggcgagac tcgcccgtgg acttgcgaag ggggagcgga
     7321 agggggctct ttttccaaga tacaggtcta tgtttctcag aaagcagatg ggcgagggag
     7381 ctggacaatc cattgtgcgg ctattggacc aaccccaaat ccagcccctt acccggagag
     7441 gttttgcgta cgcatgctat tgcttcgtcg aaaggaggag agtaatggat atcgtcacca
     7501 agatacgaaa tctctgttca tccgaagtcg actgggtgaa ggaagctgga aaagcgttcg
     7561 cggattcggc ggacgacctg ttcgatcttt tcgccgaacg aaaggcgccg tccccgaagt
     7621 tcaaggtcag gttggcgcga actttctcgg atgtgaacag cgcgcttgct gcctgtggca
     7681 tggaaaaaat agagggagaa tcggaacgcg agatcgccgc cgcgtacttc gcctactgca
     7741 aaggcgtcat cgagagcaga atgtaggttg cgcatctgga agaaccacca caaagtaatg
     7801 cgaacgaaaa ggcatttatg aatttttgac ttgtaacgct tatggcgtta ttattccctg
     7861 tggaggaggt ggttttatgg gtgcgcgcgt tacgaaaacc aagcgtcccg gattccgcag
     7921 tgtcaatgtt cggcttgttg cccgggataa ggagctggcg gagaatatct tcaggaaagt
     7981 cggtttgact cccacggagg cgattcgtct cttttaccgg cagaccatca acacgggagg
     8041 catccctttc gggctcgttt taccggaaga aactctggcc gccatgcggg aagctgagtc
     8101 cggcacggcg gaagaaatat cgctggatga tctgaaggcc gagctggatg cgatctgtta
     8161 ggcaaactag gcaaacagcg gcgttcaagc gcgattttaa acgggagatc aagagggctt
     8221 cgcgccatat tctcaaggag cgtctttggg gagtcgtcgc cgcgttgacg atggggagcc
     8281 ctctcgaccc gtcgtatcgc gatcacccgc ttggaggaaa ctggcgagga catagggagt
     8341 gtcacatcgc cccggatctg ctgttgctct attatctatc ggaagatgct cttaccttgg
     8401 tgcgtctcgg aagccatgcg gagcttttcg gactttgaag atgacaagca tgttgtaaag
     8461 aacgagtcct cgctgtcttc ttccttctcg gtctgttctc ctccaggggc ggtttatacg
     8521 ccgaccttta ttcggagcgg tagcccgagc gatgtcgcaa gttgatcaag gcgtcgagag
//
LOCUS       DAOSWW010000010         8285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_5546_length_8285_cov_3.782260, whole genome shotgun sequence.
ACCESSION   DAOSWW010000010 DAOSWW010000000
VERSION     DAOSWW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5546_length_8285_cov_3.782260"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PK442_00435"
     CDS             complement(<1..601)
                     /locus_tag="PK442_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86147.1"
                     /translation="MEKTLIIGFSVAMIVASFLIGWWAKRKVTSNKAFFGSTGMFGPL
                     AVGLSSTAAVASAFALVGVPGIIYATGNPMTFWMLSSAAFTMAYIILGKKVRAMAEIG
                     EVASLGDISDLRFNNNRLIKALLSVVIFLGCIAYLSAQIKAGSELFAHLLGWEPIVAA
                     IVIFGILTVYTAISGEVGGLLTQAFQGLVMVVAGVIMIVS"
     gene            complement(604..1047)
                     /locus_tag="PK442_00440"
     CDS             complement(604..1047)
                     /locus_tag="PK442_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86148.1"
                     /translation="MDISRKRSALMVLIAFLWLGITALGMLKLFLPAMILGVVLMFVH
                     MFLGVARKGVVSKAFLVYPLLAWGVLWGASFILSKHYSDAFAGQMPAFTILGLHPSFA
                     WTVLTYWIGGMLTLTVGFMFLRSEWLSDKDWDEFLATIASGKEGA"
     gene            1331..2293
                     /locus_tag="PK442_00445"
     CDS             1331..2293
                     /locus_tag="PK442_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOO86149.1"
                     /translation="MGDKDRAFRRLLADERLFLKFLRRFLKQNLPDVIDVEALTLDDL
                     HREDITFIPPDLSERESDVLYRIRGTDAEAYVYVLVEHQSSVSHLMPFRLLSYVVQFW
                     TRYVEKVGEASKRKDFLLPPVLPVVFYEGATRWTAPVHFAEKVRNAYDFGKFIPAFEY
                     RLISLRDKNPESLLSYGDALGALFYLANPSKTEGFLEAAERLRIFLLGLSDEERELTT
                     NHLRGYLRILMKKEGLEIDDALDLCLEREEADVMLTYMQKEFRKARKEGREEGREEGR
                     EKGQLEMGISIALAMLEAGEPEEKILRYTGLSLSQLEEIRDGKK"
     gene            complement(2755..3078)
                     /locus_tag="PK442_00450"
     CDS             complement(2755..3078)
                     /locus_tag="PK442_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86150.1"
                     /translation="MKNSTLRDPYLSIWFCQRKTDHPYTHVREVAKTAGIAVKNFYDR
                     THRYLTSLYSRPWMLKHATDAVLNGIIIEGVLQPTLFYAGALSIIGFIEEQNAVEKMI
                     REKLR"
     gene            3513..5054
                     /locus_tag="PK442_00455"
     CDS             3513..5054
                     /locus_tag="PK442_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOO86151.1"
                     /translation="MTKEELVARLKKPEWSDLECKRSRNGVSEDAYRTVSAFSNTEGG
                     SLVFGVKDSEGKLEIVGIDAPDKVQNDFLSALRTGDKLNRRINAEAERFEVENKILLV
                     FHIPEALRSEKPVYLRGDIRQSYIRRGAGDEQCLQTEIERFLRDAASERWDGLAIEYD
                     PDRCFDRRSLAWYRSRYESRPNNRSYAGVDDNEFLFQLGLIRETPQGRLPTHAAVLLF
                     GSDGYLRGMMPRPVADCQRFGFEFGENPPGVRWLDRVVMESNIVQAWLSFLDWYMKFA
                     SIPFRLDPTTMERDDTPADFIAFREAFVNVLCHQDYQDHTRKPEIRRFSDRTIFWNPG
                     DAFATGDLLAPGPKEVRNPTIAVAFRRIGFSENAGWGLNDVISQWTGLGYVRPVIRNG
                     KASKDFELMLIAASSPVSSGRIQPGEGVEQVTQQVTQQVTQQVTQQVEKLLAACTSEL
                     SRSELMKAVRIRDRVSFSRNYLEPALAESLIEMTQPDSPKSPTQKYRLTAKGRVLLES
                     VGSSK"
     gene            5280..5903
                     /locus_tag="PK442_00460"
     CDS             5280..5903
                     /locus_tag="PK442_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO86152.1"
                     /translation="MNLVKKPKTARGAETLQRICDAAEELFAESGYYNTQISDIAKKA
                     GIASGTFYIYFPDKISAFRYLLDELGRRLRREIRAAASGARTQQEAEFVGLKTFLSFA
                     SKHIGLFKIVWQAQFVDMEAFKNYYESFAARYVTRIGDAQKRGELTEMDPEYFSYILM
                     GIYNFVALKCIVFDGKEPTDELVEEVFKFIRHGAFAPSAENGAPRPQ"
     gene            complement(5980..6456)
                     /gene="grdA"
                     /locus_tag="PK442_00465"
     CDS             complement(5980..6456)
                     /gene="grdA"
                     /locus_tag="PK442_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084002417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6325..6327),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HOO86153.1"
                     /translation="MGKLTGKKLLLLGERDGVPGPAMEACLKASGASIEFSVTECFVU
                     TAAGAMDLQNQQRIKDAAEKFGAENVVVILGSSDAEGAEIYAETVSNGDPTYAGPLAG
                     VSLGLPVYHIFDGDIRNECDPAAWEEQISMMEMVLDPDALAAAVKGIRDQFSTHSL"
     gene            complement(6604..7494)
                     /locus_tag="PK442_00470"
     CDS             complement(6604..7494)
                     /locus_tag="PK442_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86154.1"
                     /translation="MFSRLFGQFLLQEGFVTPSALSSALGRLGEVRPLIGMLALAQGY
                     MSPDQVVEINEMQKRVDRRFGELAIEKGYLSVDQLESIISAQKSEHVLLGQILVQDGI
                     LSHDGFLLVLEKYRKNAGLSEEGYEAVKANDVEGVVSSILVGQPGAGKAVVRSWATVF
                     MRSVIRFIDPGAAIDPLAEEKYKNLRGFIQRMKGDLRVSVFIAAEDEVFVDLAQRFTD
                     FDIDSFDEMASAAVGEFLNLANGLFTVNCSDEGTELDMLPQEYVESIASLPIGRPDAL
                     LPLLLPKGILWAGFCPGLSR"
     gene            complement(7494..7874)
                     /locus_tag="PK442_00475"
     CDS             complement(7494..7874)
                     /locus_tag="PK442_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86155.1"
                     /translation="MEKKYTALLCDDSAVVRKMLRHILNESPVGTVFEAIDGNQAVEM
                     YRLHRPDIVFMDIVMPGKTGIQALSEIRAHDPSARVIMASSTGTSKNLKAAIDSGAVD
                     FIQKPFEREAVLKLIRRILEGEWE"
BASE COUNT         1942 a         2290 c         2345 g         1708 t
ORIGIN      
        1 aggagacgat catgatcacg cccgccacca ccatcaccag tccctggaac gcctgcgtca
       61 gcagaccgcc gacctcgccg ctgatcgcgg tgtagaccgt gaggataccg aagatgacga
      121 tagccgcgac aatcggctcc caccccagaa gatgggcgaa gagctccgaa ccggccttga
      181 tctgcgcgga gaggtaggcg atgcagccga ggaagatgac gacggagaga agggccttga
      241 tgagcctgtt gttgttgaaa cggaggtcgg agatgtcgcc caggctggcc acttcgccga
      301 tctcggccat cgcgcggacc ttcttgccga ggatgatgta ggccatggtg aaggccgcgg
      361 aggagagcat ccagaaggtc atggggttgc cggtcgcgta gatgatgccg ggaacgccca
      421 ccagagcgaa cgcgctggcc accgccgcgg tgctggagag gccgaccgcg agcggaccga
      481 acatgccggt gctgccgaag aaggccttat tggaggttac ctttctcttc gcccaccatc
      541 cgatgaggaa ggaggcgacg atcatcgcca cggaaaaccc gatgatgagt gtcttttcca
      601 tagttacgca ccctccttgc cgcttgcgat tgtcgccaga aactcgtccc agtccttatc
      661 ggacagccat tcgctcctga ggaacatgaa cccgaccgtc agcgtcagca taccaccgat
      721 ccagtaggtg agcacggtcc acgcgaaaga ggggtgaagg ccgagaatgg tgaaggccgg
      781 catctgcccc gcgaacgcgt cggaatagtg cttggagagg atgaagctcg cgccccagag
      841 cacgccccac gccagcagcg ggtagaccag aaacgccttc gagacgacgc ccttgcgggc
      901 cacgccgaga aacatgtgca cgaacataag aacaactccg aggatcattg cgggcaaaaa
      961 cagcttcaac atgcccaacg cggtgatgcc gagccatagg aacgcgatga ggaccatgag
     1021 tgcggatctt ttcctggaaa tatccattac ctatcccctc cttcgaatgt gcgacgcctc
     1081 gacggcccga tccggcacgt atccgggcgc gtcgaaatga aacatgaatc atgcttcata
     1141 atgcgcacag aatagcactc ctccctgaaa cctgtcaagg accttccgtc atttttctgt
     1201 tatttcagtc tatgaaaccg gaaattccgc cggcgcacga catgaacaac tccggaaaca
     1261 cgcgcaaagc ggcagctcca tccagcgtgc tataataatc aaaaagcttt ttgtggaaaa
     1321 gaggagtccc atgggcgaca aggacagggc gtttcgcagg cttctggccg acgagcggct
     1381 ctttctgaag tttctccgaa ggtttttgaa gcagaacctg ccggacgtta tcgatgtgga
     1441 agcgctcacg ctcgacgacc tgcatcgcga ggacatcacg ttcatccctc cggacctctc
     1501 cgagcgggaa tccgacgttt tataccgcat ccgggggacc gacgccgagg cctacgtcta
     1561 cgtgctggtg gagcatcagt cgagcgtgag ccacctgatg ccattccgtc ttctttcgta
     1621 cgttgttcag ttttggacac ggtatgtcga gaaggtcggt gaagcctcga agcgcaagga
     1681 ttttctactg cctccggtgc tgccggtagt tttctacgaa ggggcaacca ggtggactgc
     1741 gccggtgcac ttcgcggaga aggttcggaa cgcctacgac ttcggcaagt tcattccggc
     1801 ctttgagtat cggctgatct cgctgcgcga caagaacccc gaatcgcttc tctcctacgg
     1861 agacgccctg ggcgcgctct tctacctggc gaatccctcg aagacggagg gctttctgga
     1921 ggcggcggag cggctgcgga tatttctact tggcctttcg gacgaagagc gggagctgac
     1981 gacgaatcat ctgcgagggt acttgagaat cctgatgaag aaggaaggtc tggagataga
     2041 cgatgctttg gacctctgcc tggaacggga ggaggctgac gtgatgctga cctacatgca
     2101 aaaagagttt agaaaggcgc ggaaggaagg gcgagaagaa ggacgagaag aaggacgaga
     2161 gaagggacag cttgaaatgg gaatctccat cgctctggcg atgctcgaag ccggcgaacc
     2221 cgaggagaag attctccgct acaccggcct ttccctctca cagttggagg aaatacgcga
     2281 cggcaaaaag tgatcgccga ccaagacagg aggactcgac gagcaagccc ttcggagacc
     2341 gttgatcgac tatctccggg agtttgctgc atgcttcgac gatgctatcg acgaagagaa
     2401 agcgccataa gatcatcgat atatcttgca ccagcaggaa acgctatttt tttaagagca
     2461 agtacatcat tgtcaagaac tttccgaaat acattcatga agttattgtt tttttgcaga
     2521 agagcgctga atgcacctcc tcgcctcaat gcggcaagat catcgacgtt tttcccagct
     2581 tttctaaacg aattttctat tattccggaa aatacagtgt caattttcga ggcatatgcg
     2641 taactaggct gaatgcaaaa ggcgccacac atcaacaata aaataaaatt tatactggta
     2701 aattttttta aaaagacagc tcattgtact tctcctcacg aaaaaaataa aaaactatct
     2761 cagtttttct cgaatcattt tctcaacagc gttttgttcc tcgataaaac cgatgatact
     2821 cagcgctccg gcatagaata aggtcggctg caaaacgccc tctataataa taccgttgag
     2881 gaccgcgtct gttgcgtgct tcaacatcca tggtcttgaa taaaggctcg ttagatagcg
     2941 atgtgtcctg tcataaaaat ttttaacagc aataccagcc gttttagcga cttccctgac
     3001 atgagtataa gggtggtcag ttttgcgttg acaaaaccag atggaaaggt acggatctcg
     3061 caatgtgctg tttttcatat taccgaatcc cttgattgcg ttccgcttcc gtgtgccctt
     3121 gcggagcgct tcgtgcgggg cgccgggtta actacgggta aagggtatcc cggacgagcg
     3181 accacttacg acggagcgga gagcgcactt cgggcaaacg caatcggagt gcgccgcctc
     3241 cgtgctaaaa taatcggaag ttcgtttcgg aaggggctgc cgatcttgaa cgaacggtcc
     3301 ggacgaaatg ggacacatag gcggaaacag ggcgtacgaa aagggagcgg agcgggagat
     3361 attcttattg cacaggagaa tacatgcaag cgtcgcaggt attcgcagaa aatccgcacg
     3421 ttaaaaaatg atttccgcac ggtcgtattg gtccgcgtac agagtcgtgt agaggtgact
     3481 caatatttct ccgctaacct ggaggcaacg ggatgactaa agaggaactt gtcgccagac
     3541 tgaagaaacc tgaatggagc gatcttgaat gcaagagatc gcggaacggc gtttccgaag
     3601 atgcgtaccg gacggtatcc gccttctcaa atacagaggg cggcagtctg gtgttcggcg
     3661 tgaaggactc cgaaggcaaa ttggaaatcg ttggaatcga tgccccggac aaggttcaaa
     3721 acgactttct cagcgccctg cggacaggag ataaactgaa tcgtagaata aacgcggaag
     3781 cagaacgctt cgaagtcgaa aacaagattc tgcttgtctt ccatatcccc gaggcattac
     3841 gcagcgaaaa acccgtctat ctcagaggag acatcaggca gagctatatt cgccgcggcg
     3901 ccggagatga acagtgcctg caaacggaga tcgagcgatt cctgcgggat gccgcgtcgg
     3961 agcgctggga cggcctcgcc atagagtacg atccggaccg ctgtttcgac cgccgttcac
     4021 tggcctggta tcgaagccgt tacgaaagcc ggcccaataa ccgctcctac gcgggggtcg
     4081 acgataacga gttcctcttc caactgggac tgatccggga aacaccgcaa gggagacttc
     4141 ctacacacgc ggcggtttta ctcttcggtt ctgacggcta tctgcgaggc atgatgcctc
     4201 gtcccgtggc ggattgccag cgcttcggtt tcgagttcgg cgagaatccg cccggcgtac
     4261 gctggctgga ccgtgtggtc atggagtcca acatcgttca ggcgtggctc tctttcctgg
     4321 actggtatat gaaattcgcc tccatcccct ttcggctcga cccgacgacc atggagaggg
     4381 acgacactcc cgccgatttt atcgccttcc gagaggcctt cgtcaatgtg ctgtgccatc
     4441 aggactacca ggaccacacc cgtaagcccg agatccggcg cttttcggat cgcaccatct
     4501 tctggaatcc cggcgacgcc ttcgccaccg gcgatctgct cgcccccggt ccgaaggagg
     4561 ttcgcaatcc gactatcgcc gtagcgtttc gccgcatcgg tttcagtgaa aatgccggtt
     4621 ggggcttgaa cgacgtaatt tcccaatgga ccggacttgg ctatgtccgc cctgttatcc
     4681 gcaacggcaa ggcaagcaag gatttcgagt tgatgttgat cgcggcgagc tctcctgttt
     4741 cttcagggcg aatccagccg ggagagggcg tcgagcaagt aacccagcaa gtaacccagc
     4801 aagtaaccca gcaagtaacc cagcaagtag aaaaattgct ggcagcctgc acttccgagt
     4861 tgagtagatc ggaactgatg aaagctgtcc gtatcagaga tagagtcagc ttctcccgta
     4921 attatctgga gcccgctctt gcagaaagtt tgatcgaaat gacgcagccc gactcgccga
     4981 aaagccctac ccagaagtac cggctcacag ccaaaggcag agtgctcctg gagtccgtcg
     5041 gcagttcgaa atgaagtgcg cgagaagttg tggaaaagga gaccgattgt gaacaagagc
     5101 aacgcaactg acggaaggaa acggaggccc cgcccgacga gaacgttcgc aggcgtgcgt
     5161 ctcctcctct ccggagaaca gagctcacta ctctctctac tcgcttcccc gggacccgcc
     5221 ctcccgcccc ttctgtgcta tcatgtgagg cggagtaatt cgtcgaacgg ggtggtagcg
     5281 tgaatctggt caaaaaaccg aagacggcac ggggggcgga gacgctgcag cgcatctgcg
     5341 acgcggcgga ggagctgttc gcggagagcg ggtactacaa cacgcagata agcgacatcg
     5401 cgaaaaaagc ggggatcgcc tccgggacgt tctatatcta ctttcccgac aagatcagcg
     5461 cattccgcta cctgctggac gaactgggcc gccgcctcag gcgggagata cgcgccgccg
     5521 cttccggggc gcggacgcag caggaggccg agttcgtcgg gctcaagacg ttcctctcgt
     5581 tcgcttcgaa gcacatcggg ctcttcaaga tcgtctggca ggcgcagttc gtcgatatgg
     5641 aagcgttcaa gaactactac gagagcttcg ccgcccgcta cgtcacgcgc atcggcgacg
     5701 cgcagaagcg cggcgaattg acggagatgg acccggagta cttctcctac atcctgatgg
     5761 gaatctacaa cttcgtggcc ctgaagtgca tcgtcttcga cggaaaggag ccgacggacg
     5821 agcttgtgga ggaggtcttc aagttcatca ggcacggtgc gttcgcgccg tccgcagaga
     5881 acggagctcc ccggccgcag tgagccggac atgctcgcgc atatttcaac gaactaaaca
     5941 cggcccccgc gatttctcgc cgggggccgc ttcatacttt tacaacgagt gtgtgctgaa
     6001 ctggtcccga atgcctttta cggccgccgc aagggcgtcg gggtcgagaa ccatctccat
     6061 catgctgatc tgctcttccc aggcggcagg atcgcattca ttgcgaatgt cgccgtcgaa
     6121 gatatggtac acggggagtc ccaacgagac tcccgcgaga gggcctgcat aggtcggatc
     6181 gccgttgctc acggtctcgg cgtagatctc cgcgccttca gcgtcggagg aaccgaggat
     6241 gacgacaacg ttttcagctc cgaacttctc ggcagcgtcc ttgatacgct gctgattctg
     6301 caggtccatc gctcccgcgg cggttcagac gaagcactcc gtcacggaga attcgatgga
     6361 agcgccgctt gccttcagac atgcctccat ggcgggtccg gggacgccgt cccgctcacc
     6421 gaggagaagc agtttcttgc ctgtcagttt gcccattttt ccacctcctt tctgctttac
     6481 agggtagcgt caaatgagga tttcgtccag agccggcgga aaaaatcggc gggaaagaaa
     6541 aaaacggcgc cccgccgcga agggaggaga cgccgtctgc gatttcagcg cacggagagc
     6601 gcgctaccgg gagagaccgg ggcagaaccc cgcccagaga attcccttgg gaagcaggag
     6661 cggaaggagc gcgtcggggc gtcctatcgg aagcgacgcg atgctttcga cgtattcctg
     6721 ggggagcata tccagctccg tcccttcgtc ggaacagttc accgtgaaca gaccgttggc
     6781 gaggttcaaa aattcgccga ccgccgccga cgccatctcg tcgaacgagt cgatgtcgaa
     6841 atcggtaaaa cgctgtgcga gatcgacgaa gacttcgtct tcggctgcga tgaagaccga
     6901 cacgcgaagg tcgcccttca tgcgttgtat gaaccccctc agatttttgt acttctcctc
     6961 cgccagcggg tcgatggcgg cgccggggtc gatgaaccgg atgacgctcc tcataaagac
     7021 ggtggcccag ctcctgacca cagcttttcc cgctccaggc tgccctacga gaatggagga
     7081 gaccacgccc tcgacgtcgt tcgccttgac cgcctcgtac ccctcttcgg agagaccggc
     7141 gttcttgcgg tacttttcga gcaccaggag gaagccgtcg tgcgacagga tgccgtcctg
     7201 gacaagaatc tgcccgagga gcacgtgttc gctcttctgg gcggagatga tggattcaag
     7261 ctgatcgacg gagaggtacc ccttctcgat ggcgagttcg ccgaaacggc ggtcgaccct
     7321 cttctgcatc tcgttgatct cgacgacctg gtcagggctc atgtagccct gcgcaagcgc
     7381 gagcatccct ataagggggc gcacctcgcc gagacgcccc aaggcgctcg aaagagcgct
     7441 gggcgtgacg aacccctcct ggagaagaaa ctgcccgaaa agtcttgaga acactactcc
     7501 cactcccctt ccagaatacg gcgtatcagt ttgaggacgg cttcccgctc gaacggcttc
     7561 tgaatgaaat ccaccgcgcc ggagtcgatg gccgctttca agttcttgct ggtacccgtg
     7621 gacgaggcca tgatcactcg cgccgacggg tcgtgcgcac gtatttcgga aagcgcctgg
     7681 atacctgtct tccctggcat gacgatgtcc atgaaaacaa tatccggccg gtgcaagcgg
     7741 tacatctcca ccgcctgatt cccgtctatg gcttcaaaga ccgttcccac agggctttcg
     7801 tttaaaatat gacgcagcat tttgcgcacc actgcggagt cgtcgcagag aagcgctgtg
     7861 tatttttttt ccacggaaga agccctccct aatatggagt tctctcatcc aagaactctt
     7921 tttgagaatt atagcatgaa gcgcaatgtg caagcagatg gatgatgaaa tttattcact
     7981 aaagaaatat cggatattta aaaaatgttc gcgagttttt tcggcgcgtt ccgctttcgc
     8041 aaggcacgcc ttaagactcc tcgaacacag tgaatacgca tgcaaacgtg agcattgaca
     8101 aatgcaggcg ggggtattag catcgttttt gaaccgtctt gatttcacag tttatgtata
     8161 aatataaaat attttgactt tttatctagg aggcgatggc gtgcgcaacg cggtttttct
     8221 tggtccttcc ggcggaggaa agtcggagat ctccatcaac acggcgctgc aagcggcaaa
     8281 agaga
//
LOCUS       DAOSWW010000011         8273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_5555_length_8273_cov_4.105622, whole genome shotgun sequence.
ACCESSION   DAOSWW010000011 DAOSWW010000000
VERSION     DAOSWW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5555_length_8273_cov_4.105622"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PK442_00480"
     CDS             complement(<1..683)
                     /locus_tag="PK442_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO86156.1"
                     /translation="MDLVLSVVSQIDAVFVFFIFAGAVAGLFVGAIPGLSVTMATALL
                     VSITYTWSVKNALGVILGVFVSGVYAGAVSAILINIPGAPSSVVTTLDGYPMARKGEA
                     ALALWAATFHSFVGTVFGFLVLMFVAKPVTSFALTFSAMDYFLLALFGLTTIGTLTAK
                     NYIKGGICALLGLLLSMIGIDPFSGGVRLTFGVFDLQGGVPLIAALIGLFGFSEVLDQ
                     VARGAMNAIA"
     gene            complement(696..1151)
                     /locus_tag="PK442_00485"
     CDS             complement(696..1151)
                     /locus_tag="PK442_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOO86157.1"
                     /translation="MNTSPWAANMRHSLFFLVLAALFGWQGARSHDGDDWALSPALFP
                     LLLSSALALLALALLLQGYRRLKNGVPAVLPQGADFSTGRVFLVFGLCCAYALLLPWA
                     GFFPSTALFLVIFSLMIGERRLWPIVLLSFLSPAAILLIFRHGLNVLLP"
     gene            complement(1229..2242)
                     /locus_tag="PK442_00490"
     CDS             complement(1229..2242)
                     /locus_tag="PK442_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOO86158.1"
                     /translation="MKKVWKLGVLVFIVAAMLSVPAGGFADEYPSKGITLVVPWGAGG
                     VTDVAARVFAPLFEKYMGVPVVILNRPGASGAIGTEYAYARPADGYTVLFSAETPASF
                     RIMDVSKLSFHDFEPIMLLSHSEKIIAVAPESPYQTLADLVNDIRARPGKVRFAYSGA
                     GASGHVQGLLMMQKGNLDFSSTPFGGGNNALLAVLGGQVDFTSPNIGTVKDYISSGKL
                     RALAVFDSKKTAYLPELPPITDAIPEIAPYLPLDYPNCLAVKKGTPPEVVEKIADATS
                     KAVADPKWKEFLENNWYVGLDAVRGQDMLVYWDKWASVVGWVLQDAGVAKKSPEAFNI
                     PRP"
     gene            complement(2618..3937)
                     /gene="xylA"
                     /locus_tag="PK442_00495"
     CDS             complement(2618..3937)
                     /gene="xylA"
                     /locus_tag="PK442_00495"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF003998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOO86159.1"
                     /translation="MGDLFPGIKKIQYEGPLSQNPLAFKEYNADEVVAGKPMKEHFRF
                     AMSWWHTICSSGSDMFGSGTAIRPWDYEPHPVKRAKLAADGAFEFMEKLGLEYFCFHD
                     RDIAPEALTLAETNERLDEVCDYVKGLMSKTGIKLLWGTANLFSHPRYMHGAATSCNA
                     DVFAYAAAQVKKAMTLTRDMGGVNYVFWGGREGYETLLNTNMSLELDNLARFLKMAVA
                     YAKEIGFTGQFLIEPKPKEPTKHQYDYDVPAVLAFLRKYGLEKDFKINLEANHATLAG
                     HTFHHELHIARINGVLGSIDANQGDLFLGWDTDNFPTDVNAATLAMVEVLKNGGIAPG
                     GFNFDAHVRRPSFQDDDLFIAYIVGMDTFAKGLKVAAKLIEGGEIEAFVAKRYESYSS
                     GVGKKIADASVGFRELEAYALAHDQPVNGSGRQEMLEAIVNRYMLNP"
     gene            complement(4023..5216)
                     /locus_tag="PK442_00500"
     CDS             complement(4023..5216)
                     /locus_tag="PK442_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOO86160.1"
                     /translation="MRIRSINAEKMGLSNRLTLLNLIRTTPGVSRRELARLAGLDPST
                     VTKIITSLLEKGLVIEHGVRDADHPGRKSIMLRVYRDAAVALIAEIGVEKTRIAKGFL
                     DASFEVLDEFDTPPKEDEFFSVFAGSLRKKAFSFPHTAYCALSVAVPGMVEWGSNVLR
                     SLPHMGWKNVDFEARLGKLLPEMELTVFTANEAKLSLIAEMFRNGGVSGLHSGVYVYL
                     SQGVGGAILMGDKIIHGLSNTAGEIGHMTVDPSGPLCHCGNRGCFETFVSIDTVVRNF
                     ENLGASLPGSDFREKFRALLRLFQEGNDAARRVLEEMGDFLATGIANLVSILNPDFIM
                     LGGMGSILPPSFVAGVGENVAKKTPGATSPDFLLTTASLGIESAALEGATLMAMNHYV
                     KKAVK"
     gene            complement(5341..6078)
                     /locus_tag="PK442_00505"
     CDS             complement(5341..6078)
                     /locus_tag="PK442_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOO86161.1"
                     /translation="MFEAEYSIPYAGIGPDGAMKLPVLLDILQDMADRDAHRMSMTVA
                     DLLPRGLSWVLRQYRVVVDRYPGHESMLKVRTWHGPRRNLHSIRAFEVRDAAGPVASA
                     WTSWILIDTLRGRPLRLDRHTTEVYTREAYPMDGDAPSLPVVEDFAYDTFFSVRRWDL
                     DRNAHVNNAVYFSWAVESAPDAVAAEYELVKVEAEYLKPVEKNGDVRVRTALLPADQG
                     EKAFFHLIEGPDGEVKAHFATRWRGRK"
     gene            6365..6838
                     /locus_tag="PK442_00510"
                     /pseudo
     CDS             6365..6838
                     /locus_tag="PK442_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            6962..7330
                     /locus_tag="PK442_00515"
     CDS             6962..7330
                     /locus_tag="PK442_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOO86162.1"
                     /translation="MRLTWPLRGGPPPTHTGTSFGEGLSAKAAPGEPVHAAADATVLH
                     GGWMRNFGNAIYLNHGNGIATFYGGLGIIYVKSGEKIRRGTKIALVGEIQGAEPRFVF
                     HVLKEGKSVDPRPWIEGAKQ"
     gene            7767..8240
                     /locus_tag="PK442_00520"
     CDS             7767..8240
                     /locus_tag="PK442_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO86163.1"
                     /translation="MIIELTVNGTLRSVEITPSMRLLDMLRNVLGLTSVKEGCSEGEC
                     GACTVLLGGKAVTSCTVLAVQARGKSVVTIEGLSSDGALHPIQQAFVDHDAIQCGFCT
                     PGMIMSAYALLKHTPSPTREEIQRGIEGNLCRCTGYLPIIDAIEDAARRMRTHES"
BASE COUNT         1907 a         2419 c         2284 g         1663 t
ORIGIN      
        1 gcgatggcgt tcatcgcacc gcgcgccact tgatccagca cctccgagaa tccgaaaagg
       61 cctatgagcg cggcgataag tggaacccct ccctggagat cgaatacgcc gaatgtcagc
      121 cggacgccgc cggagaaggg atcgatgcct atcatgctca acagcaggcc cagaagggcg
      181 caaatgcctc ccttgatata attcttcgcc gtcaacgtac ctattgtggt caagccgaaa
      241 agcgccagca aaaaatagtc catcgcggaa aaagtaagcg cgaagctcgt cacgggcttg
      301 gcgacgaaca tcaggacaag aaagccgaag acggtgccga cgaaggagtg gaaggtcgct
      361 gcccagagag cgagcgccgc ctcgcctttg cgggccatcg gatacccgtc aagcgttgtc
      421 actaccgagg aaggagcccc cggaatattg atcagaatgg cggagaccgc gcctgcgtac
      481 accccggaga caaagacccc gaggatgact ccgagcgcgt tcttcacact ccatgtatag
      541 gtaatagaca caaggagcgc cgtcgccatt gtcacggaaa ggccggggat agcccccacg
      601 aagagccccg ctacagcccc ggcgaaaata aagaacacaa agacggcgtc gatttgagaa
      661 acgactgaga gcacaaggtc cattgattct tcctcctatg gaagcagcac gtttaaaccg
      721 tggcgaaaga tgaggagaat cgccgccgga gaaagaaacg aaaggagcac aataggccaa
      781 agacggcgct caccgatcat cagggagaaa atcacgagaa aaagcgccgt agaggggaag
      841 aagcccgccc agggaagcaa gagagcgtag gcgcaacata agccgaaaac gagaaatacc
      901 cttcccgtcg agaaatccgc cccctgcggc aataccgcag gcacgccatt ttttagtcgg
      961 cggtagcctt gcaggagcag cgcaagggcg agtagagcaa gcgccgacga gagaagcaga
     1021 ggaaaaagcg cgggagaaag cgcccagtcg tctccgtcgt gcgagcgcgc tccctgccaa
     1081 ccgaaaagcg cggcaaggac gaggaagaac aggctgtgcc gcatatttgc cgcccaggga
     1141 gaagtgttca taaaaagcca tcctttcatg aaagcaaaag agccgccact cccttgccgg
     1201 gaaggggcgg ctcgtcgtga agcgagtttt acggtctggg aatattgaac gcttcggggc
     1261 tctttttcgc cactccggcg tcctgcagca cccatcccac cacggaggcc cacttgtccc
     1321 agtagacaag catatcctgg cctcgcacag catccagccc gacataccag ttgttctcca
     1381 ggaactcctt ccatttcggg tcggcaacag ctttcgacgt cgcgtcggcg atcttctcta
     1441 caacctccgg aggagttccc tttttcaccg caaggcaatt cgggtagtca agcggcagat
     1501 agggggcgat ctcaggaatg gcgtcggtga taggaggcag ttcaggcagg taggcggtct
     1561 tcttgctgtc gaagaccgca agcgcgcgga gtttccctga ggagatgtag tccttcacag
     1621 tcccgatgtt gggggacgtg aaatcgacct ggccgccgag gaccgcgagc agcgcgttgt
     1681 ttccgcctcc gaagggagtg ctcgaaaagt cgaggtttcc cttctgcatc atcaggagcc
     1741 cctggacgtg accgctggcg cccgcgccgc tgtaggcgaa gcggactttt ccgggccgcg
     1801 cgcggatatc gtttaccagg tccgcaaggg tctggtacgg cgactccggc gcgaccgcga
     1861 tgatcttttc cgaatggctc agcagcatga tcggctcgaa gtcatggaag gagagcttac
     1921 tcacgtccat gatccggaac gacgcaggcg tctcggccga gaagagcacc gtatatccgt
     1981 cggcaggacg ggcataggca tactctgtgc cgattgcgcc ggaagcgccg ggccggttga
     2041 ggataaccac ggggacgccc atgtatttct cgaagagagg cgcaaagacc ctcgccgcca
     2101 cgtcggtgac gcccccggcc ccccaaggaa ctacaagcgt tatccccttc gacggatact
     2161 cgtctgcaaa accgccggcc gggacggaaa gcatcgccgc tacaataaag accaggacgc
     2221 ccagtttcca cacctttttc atctgcaaca ctccttttgt tctttcgtct ttctccatag
     2281 agaagatgag aggaatacaa ccgtttcatc caatgaacgc aacgcgtttc ggttgagggt
     2341 gagcgacttc cacccgccaa aacataactt aggtacacta ccgatcaaaa gaatgaataa
     2401 cggttgaagg cattataaac tatcaacata ctaccttaca agagcatcag gaagaagaga
     2461 aaactcgccg gaactaaaaa accgtacgcc gatggacatt ccggttgtgc tgccggcagc
     2521 gctccgtgaa aaaaaccgac cggttcttca gcttcgcgaa agatgaaccg gtcggaaacg
     2581 actccatttc tggattttct acgagccgta tggttgttta cgggttgagc atatagcggt
     2641 tcacgatcgc ttcgagcatc tcctgccgtc cggagccgtt gacaggctga tcgtgcgcga
     2701 gcgcgtacgc ttcaagctcc cggaatccga cggaagcgtc cgcaatcttc tttcccactc
     2761 cagacgagta gctttcgtac cgttttgcca caaacgcctc gatctcgccg ccctcgatca
     2821 gtttggccgc gaccttcagc cccttggcaa aggtatccat cccgacgatg tacgcgatga
     2881 agagatcgtc gtcctggaac gaaggacgac ggacatgcgc gtcgaagtta aagccgcccg
     2941 gagcaatacc gccgttcttc aggacttcga ccatcgcgag cgtcgccgcg ttcacgtccg
     3001 tcgggaagtt gtcggtatcc catccgagga agagatcgcc ctggttcgcg tcaatgctgc
     3061 cgagaacgcc gttgattctg gcgatatgca gttcgtgatg gaaggtgtgg cccgcgagcg
     3121 ttgcgtgatt cgcctccaga ttgatcttga aatccttctc cagaccgtac ttcctcagga
     3181 atgccagtac ggcggggacg tcatagtcat actgatgctt ggtaggctcc ttcggcttcg
     3241 gctcgatgag gaactgtccc gtgaaaccaa tctccttcgc gtaggctacc gccatcttga
     3301 ggaagcgggc cagattgtcg agttcaaggc tcatattggt gttcagcaga gtctcgtacc
     3361 cctcccggcc cccccaaaag acgtagttca caccacccat gtcgcgggtg agggtcatgg
     3421 cctttttgac ttgggcggcg gcataggcaa agacgtccgc gttgcacgat gtggcggcgc
     3481 cgtgcatgta tcgcgggtga ctaaagaggt tcgcagtccc ccaaaggagc ttgattcccg
     3541 tcttgctcat cagccccttg acatagtcgc aaacttcgtc gaggcgctcg ttcgtctcgg
     3601 caagcgtcaa cgcctcggga gcgatatccc tgtcgtgaaa acagaaatac tcgagcccca
     3661 gcttctccat gaactcgaaa gcgccgtccg ccgcgagctt cgcccgcttg accgggtgcg
     3721 gctcgtaatc ccacggacgg atagcggttc ccgagccgaa catatcgctt cccgaagagc
     3781 atattgtgtg ccaccatgac atggcgaagc ggaagtgttc cttcataggc tttccggcga
     3841 cgacttcgtc ggcgttgtac tccttgaaag cgagcggatt ttgtgaaagc ggcccctcat
     3901 attgaatctt ctttattccg gggaagagat ctcccattgt atttacctcc ttttcagagt
     3961 gcagtttctt ttttccttga cgccctcaca cgagaacatc tccttggatt cggcgccttt
     4021 cttcatttca ctgctttctt cacataatgg ttcatcgcca tgagcgtcgc tccttcaaga
     4081 gcggcggatt ctatacccaa tgaagcggtg gtgagcaaga aatccgggga agtcgcgccg
     4141 ggcgtttttt ttgcgacgtt ttctccgaca cccgctacaa acgagggggg cagaatggat
     4201 cccattcctc caagcatgat gaagtcggga ttaaggatac tgacaaggtt ggcgatacct
     4261 gttgcgagaa agtctcccat ctcctcgaga acacggcgcg cagcgtcgtt tccttcctgg
     4321 aacagcctga gaagcgctct gaatttttcg cggaaatcgc ttcccggcaa ggaggctccc
     4381 aggttctcga agttcctcac aacagtgtct atggaaacga acgtctcgaa acaccctcgg
     4441 ttgccgcagt ggcagagagg gcctgaagga tcgaccgtca tatgtccgat ttcacccgcc
     4501 gtattgctga gtccatggat gatcttatcc cccatgagga tggcgcctcc caccccctgc
     4561 gaaagataga catagacgcc gctgtgcagc ccggagactc cgccgttccg gaacatctcg
     4621 gcgatcaggg agagtttggc ctcgtttgcg gtgaacacag tgagctccat ttcgggaagc
     4681 aacttcccga gacgcgcttc aaaatcgacg tttttccacc ccatatgcgg aagagagcgc
     4741 agcacgttgc tcccccactc caccattccc ggaacggcga cgctcagcgc acaatacgcc
     4801 gtatgaggga aggagaacgc tttctttcgc aggctcccgg cgaaaacaga gaaaaattcg
     4861 tcctccttcg gcggcgtgtc aaattcgtca agcacttcaa aagaagcgtc gagaaaccct
     4921 ttcgcgatcc gggttttctc gacgccgatt tctgcaatca gcgcaacggc agcatcacgg
     4981 tatacgcgga gcatgatgga ctttctcccc ggatggtcgg cgtcgcgcac tccatgttca
     5041 atgacgagcc ctttttcaag gagcgaggtg ataatcttgg tcactgtgct gggatcaagc
     5101 ccggcgagac gggcaagttc ccgcctgctc acaccgggag tggttcggat aaggttgagc
     5161 aaggtcagtc tgttcgaaag acccattttt tcagcgttga tgctccggat tctcatcccg
     5221 tccctcctct gcgtcgaatc aatacggcgg ctcgcggtac ggagattagt ttattgatga
     5281 aacaaaccaa ttctagagag aaagatgaga gttgtcaatg gcgaattttc tttcagaagt
     5341 ttatttccgg cctctccacc gtgtggcgaa atgcgccttc acttccccgt ccggcccttc
     5401 gattaagtga aagaaagcct tttcgccttg atcggccgga agcaacgcgg tcctgacgcg
     5461 cacgtcgccg tttttctcca cgggtttcag gtattcggcc tccactttta ccagctcgta
     5521 ctccgcggcc actgcatccg gcgcagattc gacagcccaa gagaagtaga cggcgttgtt
     5581 cacatgggcg tttcggtcaa gatcccatct gcggacggag aaaaatgtat cgtaggcaaa
     5641 gtcctcaact acaggaagag aaggagcgtc tccgtccatc gggtaggcct ctctcgtgta
     5701 gacctccgtg gtgtgccggt caagcctgag gggacgtccg cgcagagtat cgataaggat
     5761 ccagcttgtc catgcggacg caacgggtcc ggcggcgtcg cgaacctcga aagcgcggat
     5821 cgaatgcaga tttctccgcg gtccgtgcca cgttctgacc ttcaacatac tctcgtgccc
     5881 gggatagcgg tccacgacga ccctgtactg acgcagcacc cagctcaacc ctctcggcag
     5941 aagatcggcc acggtcatgg acatcctgtg cgcatcccgg tcggccatgt cctgcaatat
     6001 atcgagaagg acgggaagct tcatggcccc gtccggtcca atccccgcgt agggtatgga
     6061 gtattcggct tcaaacacgt aatcatctcg tttctctgga atataatatg cgaagggaac
     6121 aggacggaac gtgccgcatc ttttccccag cgtcgctttt tcgaggtata ttatagtaca
     6181 gaacgagttt cttctctccc gtgcggaaga acaggctccc gccgggcgaa tatcatgtcc
     6241 ttcgggacgc caggaggttg aacagagcat gagacgcata ctcttcctca cacttcttct
     6301 cgttattacg gccgcgcctt ccgttatcgc ggaatagccc ccgttcggcg tctacacggt
     6361 aaaagccgga gacaccgtag actcggtagc cgggaggtac ggcgtcgacc ctgcgaacct
     6421 cgcctcagcc aacgggatag ctgcgaacga tggtcttcca ccggagggga cggtgctgct
     6481 ggttcccaaa tcaccgggcg acgttcttgc caccctctac gaggcgaaac gccgcggcct
     6541 gggagcatgg ccgaagccga aatacgccga tgagtttctg ccgcccttga cagaagaacc
     6601 gccctcggac aagaagcctt cggccccgga ggagcgcccg cagaaagccg aacacacacc
     6661 cgctccggca gtatcttctc aacagcctgc ggctccggcg gacggaacgg tacgcaccca
     6721 taaagtacag gagggagaga ccatgtacgg aatttcgcgt ctctacggcc tctcgctgtc
     6781 cgcgctgctg caagccaaca atcttaccga aagttccatt atcaagatag gcgagacgct
     6841 cacgattccc ggaacctcgg ccgaaaagcc ggaacctcat gtgcagcgcc cgaccccgcc
     6901 gcagaacgag ccgatcgtcg aaaagacgcc tgcagaagcg cctccatcac ctaccacacc
     6961 gatgcgcctg acctggcctc tccgtggagg tcctcccccc acgcataccg gaacgagctt
     7021 cggagaaggg ctttccgcaa aggctgcccc cggcgaaccg gttcatgcgg cggcggatgc
     7081 gacagtcctt cacggcggat ggatgcgaaa tttcggcaac gcgatctatc tgaaccacgg
     7141 caatggtatt gccacttttt acggaggact cgggatcatc tacgtaaaat ccggagagaa
     7201 gatacggcgt ggaacaaaga tcgcccttgt cggagagatt cagggagccg agccgaggtt
     7261 tgtcttccat gtcttaaagg aaggaaaatc cgtcgatccc agaccctgga tagaaggcgc
     7321 aaaacagtaa ccgtttttca gacgcggacc tcacagcgcg ggggagagtg tcgccggacg
     7381 acttcccccc gacgctgcaa gtccgccctc cgctcagtaa cgaagtagct gctccataag
     7441 aaccgccctc ggtgtcattc atttcttcaa gtcatctgga caaaaagcga gagtcataac
     7501 acatcctccg gagaagaata cccgtgaatc cacagagaaa aagtgagcag ctcaaatact
     7561 ccgctgcatt ctcttctttc ggcatgattg gcttttgccc cgatgctttc ccccggcact
     7621 gaagatcgtg tgctcttttg acgttttctt gacgagtttt tgacagggta atagatagaa
     7681 tttcgctggc atcatcctcc gaagaatgta ccaaaagggg aaaatgagaa cgcacgagaa
     7741 cagaattatc gccgggagga attgtagtga tcatagaact gacagtcaac ggaacgttgc
     7801 gctcggtcga gataaccccg tcgatgcgcc ttctggatat gctgcggaac gtcctcggac
     7861 tcaccagcgt caaagagggg tgctccgagg gggagtgcgg cgcgtgcacc gtgctgctcg
     7921 gcggcaaggc ggtaacctcc tgcactgtgc tcgcggtgca ggcgagagga aaatccgtcg
     7981 tcactataga aggactctcc tccgacggtg cgcttcaccc gattcaacag gcgttcgtcg
     8041 accacgacgc tatccagtgc ggtttctgca cacccgggat gatcatgtcc gcgtacgcgc
     8101 tgctgaagca caccccctcc cccacgcgcg aagagattca gcgcggcatc gaggggaatc
     8161 tctgccgctg caccggctat cttccgatta tcgacgccat cgaggacgcg gcaaggagga
     8221 tgaggacgca tgagagttga caccccctcg aatgtgaacg aactccggga tgt
//
LOCUS       DAOSWW010000012         8216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_5626_length_8216_cov_4.225708, whole genome shotgun sequence.
ACCESSION   DAOSWW010000012 DAOSWW010000000
VERSION     DAOSWW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5626_length_8216_cov_4.225708"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="PK442_00525"
     CDS             <1..128
                     /locus_tag="PK442_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HOO86164.1"
                     /translation="IRTSSMVMRSKSGTIRFVDSIHVPKKLSELSKVSGIDYSTV"
     gene            complement(200..2503)
                     /gene="hypF"
                     /locus_tag="PK442_00530"
     CDS             complement(200..2503)
                     /gene="hypF"
                     /locus_tag="PK442_00530"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOO86165.1"
                     /translation="MLFQTRILVSGIVQGVGFRPFCVRLANSESISGYALNTSHGVEM
                     ELHGEEENLDSFLFRIVRENPPASFISSVAILDEKRKSSSPPARFEIMESRKETLQTA
                     LIPADLAVCAQCLAEMKDPENRRYRYPFINCTDCGPRYTIIRELPYDRPKTTMASFVM
                     CPKCSSEYADLEHRRFHAEPNACPLCGPEVWLTDSNGTELTRGDEAVRLCSSLLDQGK
                     IVAIKGIGGFHIACTPFSDETVALLRDRKRRAHKPFALMVSSLEIAEELTYLPAVAKR
                     LMESPASPVVLCCVRPKPRSSDVAAVSPLVAPGQRTLGLMLPYTPVHHLLLEGKRALI
                     MTSANFSDAPIVSDNAAALASLSSIADFFLFSNRDIEMPIDDSVAMPFGRSFFLLRRA
                     RGYTPLPMNFRRGDSCVIFGAGAEMKGSFCFSREDVLLPGQYLGDMKQRDTVKYYNRA
                     LEHFVKLYELKPGKVAFDMHPQYLSTQIAKKVAENYSAETVPVQHHHAHFAACLFENN
                     VEETSAGILFDGTGYGEDGTIWGGEIFIGNRSRYERIGHFYPSRLPGGDAAVMEPWRY
                     ALGLLHDVYGKEEASVMGERLWSAHSRKVRLVLDILPSSSETTSCGRFFDAAAALLGC
                     GEVVTFDGQAAMSLEAMARGTLIAPFLVTRQDGRFIIDWRPVIRWILDSMETMPAPDI
                     AGGIHGGLARAVADICGTLRKERGIRYAALSGGVWQNRRLAAITSIFLRRRGVEPLLH
                     KLLPPNDECVSAGQTVVAAEFFNHEER"
     gene            2576..3103
                     /locus_tag="PK442_00535"
     CDS             2576..3103
                     /locus_tag="PK442_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOO86166.1"
                     /translation="MPILEYGLVHVYMGHGKGKTTSALGIVLRMAGCGGKSAVVQFMK
                     GWPYSEAKGLQFLPGVTLFQTGRADYVYPDKIDPLDYSEAERGLSIGAEAVFSGEYDL
                     VVLDELNVALSFGLVPLERVLALIERKPPHVELVFTGRNPPQELIDRADLITEMIEIR
                     HPYQKGILARKGIDC"
     gene            3197..5689
                     /locus_tag="PK442_00540"
     CDS             3197..5689
                     /locus_tag="PK442_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86167.1"
                     /translation="MTKATTNPSRKGSKITAGSAQNKSLTIEQLRKKTDAASLGISTT
                     NEIAPLEEFLGQKRAVSSISFGLEVESKGYNIVVLGNPGSGRTTYALERLRTAARKRT
                     PPDDWVYVYNFEDPGQPLAINLPAGKGKEMGTAFDEMVEELKTSISKAFEKSQYEDAK
                     AQLVKEFQEEVNQLMDEVKAWAAENGFSLKRTPQGFVNIPLTEEETESGEKTKREIQQ
                     EEFEALSEEEQKSLQKKSEEVSQKTLETLRKIRDLEKGLKERIVKLEAEICRNAIMPY
                     LSDIKEKFGTNGDSSKDENQKSNDVLGSWIDALAEDIIDNFGMFVAAVKDENAEIDFT
                     RYTVNVFVCNDPENGAPVIWETNPTYYNLGGKVEYESRQGYLYTDFKKIVPGAFQKAN
                     GGFLVLDAEKVLLNFMSWEALKRILRTGEATIENLGEQYGAVPVSSLRPQPIPINMKV
                     VMVGTPFLHALLQYYDPEFQKMFKIKADFDTDMERTPETEKQMAQFIASCLKKENGLP
                     FDASGVAEVIDCSARIADDQGRISTEFNKITEIIVESTAWAKAENADTVQREHVKKAI
                     REKRFRSDLIQEKIARAYQDGLVRIDTSGKVVGQINGLSVIDLMDYRFGHPSRITANV
                     FMGQDGVVNIEREVKMTGPIHNKGLLILTSYMGRKYAQDMPLSLSAKLTFEQMYGGIE
                     GDSASSTELYCLLSALSEVPLKQGIAVTGSVDQFGNIQPIGGVNEKIEGFFEYCEHGG
                     LTGEQGVLIPVQNTRHLMLDDTVIEAVSKGKFFIRAVSTIDEGIEALTGVSAESIHRK
                     VKNKLKKWAKKTEKLKKGKDSEAGGDDDSEEE"
     gene            5670..6374
                     /locus_tag="PK442_00545"
     CDS             5670..6374
                     /locus_tag="PK442_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOO86168.1"
                     /translation="MTPKKNSKKEPPSRPQDIDTVFDILEELWGNEKNEPRLGHEEPL
                     DGLILTLLSQNTNDRNRDKGFAALKNSFPSWELVASASAEAIADRIRPAGLAKTKSER
                     MLQILAALKERFGEYSLMALKDMDNDAVREYLSSLPGIGAKTVACVMLFDLGMSAFPV
                     DTHIARFCRRMKWAAESLPPDEIQPLLEEWVPERRFLGGHVNIIEHGRGLCHARTPEC
                     GKCPLHSNNLCPSVQE"
     gene            complement(6444..6773)
                     /locus_tag="PK442_00550"
     CDS             complement(6444..6773)
                     /locus_tag="PK442_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HOO86169.1"
                     /translation="MTEKWKDKLTDQLCRALLSLETPDEVYCFLEDVATIGEIRALAQ
                     RLEVARLLSEAYTYPQIAQQTGASTATISRVKKFLEYGADGYKIVLDRLKEAGEATNP
                     EDKSSKA"
BASE COUNT         2193 a         2134 c         2140 g         1749 t
ORIGIN      
        1 aaattcgcac cagctcaatg gtcatgcgct caaaaagcgg cactatccgt tttgtggaca
       61 gcatccatgt cccgaaaaaa ctctcggagc tctccaaggt aagcggaata gactacagta
      121 cggtttgaca cacagccctt aagagaagcg gaaaccctct tttgcctgcg ggacactctg
      181 catatcccgc aggcgttttt tatcgctcct catgattgaa aaattccgcc gcaaccacag
      241 tttgccccgc agagacgcac tcgtcgttcg ggggcaagag tttgtggaga agaggctcca
      301 ctcctcgccg ccgtaagaaa atggatgtta tcgccgcgag tcgccgattc tgccatactc
      361 cgcccgaaag ggcagcataa cgaatccctc gctctttacg gagcgtaccg caaatatccg
      421 cgactgcccg ggcaagaccg ccatggatgc ctcccgcgat atccggagca ggcatagtct
      481 ccatcgaatc gagaatccaa cggatcacag gcctccaatc gataatgaaa cggccgtctt
      541 gtcgcgtaac aagaaaagga gctatcagcg ttcctctggc cattgcctcc agactcatcg
      601 ccgcctgtcc gtcaaaagtg acgacctctc cgcaccccag caaagctgcg gcagcatcaa
      661 agaagcgtcc gcaggatgtc gtttccgagg aggaggggag aatgtcgaga accagtcgta
      721 ctttccgtga atgagcgctc cacagccttt ctcccatgac agaggcctcc tccttcccgt
      781 atacgtcgtg caaaagtcca agagcatagc gccatggttc cattacagcc gcgtctcctc
      841 cgggaagccg cgaggggtaa aaatgcccta tccgctcata acgcgatctg tttccgataa
      901 aaatctcccc gccccagata gtgccgtcct cgccgtatcc agtaccatca aagagtattc
      961 cggcagaagt ctcttcgacg ttgttttcaa aaagacacgc cgcgaagtgc gcatgatgat
     1021 gctgaaccgg tacagtttcg gcggagtaat tttcggcaac ctttttcgcg atctgcgtag
     1081 aaagatactg ggggtgcata tcgaaagcga ccttccctgg tttcagctcg tagagtttca
     1141 cgaagtgctc cagcgccctg ttgtagtact tcacggtgtc gcgctgtttc atgtcgccaa
     1201 gatactgccc ggggagcagc acgtcctccc gggagaaaca gaagctccct ttcatctcgg
     1261 cgcccgcacc gaagataacg cagctgtctc cacggcggaa attcatcgga agcggagtgt
     1321 accctctggc gcgccgcagg aggaaaaatg aacgtccaaa cggcatcgca acggaatcgt
     1381 cgatgggcat ctcgatatcg cgattcgaaa aaagaaaaaa gtccgcgatt gaggagagcg
     1441 aggcaagagc cgcagcattg tcggagacga tcggggcgtc cgaaaagttc gcgctggtca
     1501 tgataagcgc cctctttcct tcgagaagca gatgatgcac cggcgtgtac gggagcatta
     1561 atccaagagt ccgctgcccg ggagcaacaa gaggagatac agcggcgaca tcggaggaac
     1621 gcggcttcgg gcgaacgcag cagagcacca caggacttgc gggagattcc atgagtctct
     1681 ttgcgacagc gggcagatag gtaagctcct ctgcaatttc aagagaagaa accatcagcg
     1741 caaacggttt atgcgctctt cgcttccggt ctcgcaacaa ggcaactgtc tcgtcactga
     1801 aaggggtaca cgcaatatga aaaccgccta taccttttat tgcaacgatt ttgccctggt
     1861 caagcagcga ggaacaaagt cggacagctt cgtcccccct tgtcagctcc gttccattcg
     1921 aatccgtaag ccagacctcc ggaccgcaga gggggcacgc attcggctcg gcatgaaaac
     1981 gcctgtgctc caaatccgca tactcactgc tgcacttggg gcacataaca aacgaagcca
     2041 ttgtggtctt cggacgatca tagggaagtt cgcgtataat agtgtaacgg ggaccacagt
     2101 cggtacagtt gataaaaggg tagcgatacc ggcggttttc cggatccttc atctcggcaa
     2161 gacactgtgc gcacacagca agatcggccg gaatcagggc ggtctgcagt gtttcttttc
     2221 ggctctccat tatctcaaaa cgagcgggcg gactcgaaga cttccgtttc tcgtcaagga
     2281 ttgcaacgga gctgatgaag gacgccggag ggttttcccg aacgattctg aagaggaagc
     2341 tgtcaagatt ctcttcctcg ccatgcagtt ccatctcgac gccgtgcgag gtattcagcg
     2401 cataccctga aatactctcg gaattcgcaa gacgaacaca aaaggggcga aaccctacgc
     2461 cctggactat acctgaaaca agaattcgcg tttgaaaaag cattgacaaa cctccgttct
     2521 gaaggcattg taatgcatat ggaggggaaa tgcatagatg gacgggggag atacaatgcc
     2581 gatacttgaa tacggtttgg ttcacgtgta tatgggacac ggcaaaggga agaccacaag
     2641 cgcgctcggt atcgtgcttc gaatggctgg atgcggaggg aaatccgctg tcgtccagtt
     2701 catgaaaggc tggccgtaca gcgaagcaaa agggcttcag tttctccccg gagtgacgct
     2761 cttccagacg gggagagcag attacgtgta tccggacaag atagatcctt tggattacag
     2821 cgaggcggag cgcggccttt ccatcggcgc cgaagcggtg ttttcggggg agtatgacct
     2881 cgtggttctt gacgaactga atgtcgcgct ttcgttcggg cttgtcccgc tagagcgggt
     2941 gcttgctctt attgaacgta aaccgcctca tgtggagctc gtttttacgg ggagaaatcc
     3001 tccgcaggaa ctcattgacc gagccgatct cattacagaa atgattgaaa tccggcaccc
     3061 ttatcaaaaa ggtattcttg cccgcaaggg gatcgattgc tgagaagttt cttgcccttc
     3121 tcacgaaaac gaaacagatc tgttctctcg tgaatgcata caaacaatga aattgacatc
     3181 tctggaggta gcatctatga caaaagccac gacgaacccc tcccgcaagg gatcaaaaat
     3241 aacagcaggg agcgctcaaa acaaatcgct cacaattgag cagcttcgga aaaaaaccga
     3301 tgccgcctcg ctcggcattt caacgacgaa cgaaattgca cctcttgagg agttccttgg
     3361 tcagaaacgg gcggtatcct cgatctcctt cggcctcgaa gttgagagca agggatacaa
     3421 catcgtcgtg ctcgggaatc ccggcagcgg tagaacaacg tatgctcttg aacgcctccg
     3481 cacagcagct cgcaaacgca ctcctcccga cgactgggtg tatgtatata actttgagga
     3541 cccggggcag ccacttgcta tcaacctccc ggcaggcaaa gggaaagaaa tgggtacggc
     3601 gttcgacgaa atggtcgaag agctgaagac atcgataagc aaggcgttcg aaaaaagtca
     3661 gtacgaagac gccaaagcac agctcgtcaa agagtttcag gaagaagtca atcaactcat
     3721 ggatgaagtc aaggcctggg ctgctgaaaa cggtttttcc ttgaagagga ccccgcaggg
     3781 ttttgttaat atccccttga cggaagaaga aacggagagc ggagaaaaaa caaagcggga
     3841 gattcagcag gaagaatttg aggcgctctc cgaggaggaa cagaaaagtc tccagaagaa
     3901 atcggaggag gtttcccaaa agacgcttga aacgctccga aagatccgcg accttgaaaa
     3961 aggactgaag gaacgcattg tcaaactaga ggctgaaata tgccgcaatg caatcatgcc
     4021 ctatctttcc gacatcaaag aaaagtttgg aacgaacggg gattcctcca aggatgagaa
     4081 tcaaaagagc aacgacgttc ttggaagctg gatcgacgcc cttgccgaag atatcattga
     4141 caacttcggc atgtttgtgg cggcggtcaa agatgaaaac gccgagatcg attttacccg
     4201 gtacacagta aacgtttttg tctgcaacga tcccgagaac ggcgctcccg ttatttggga
     4261 gacaaaccct acatactaca atttgggcgg aaaggttgaa tacgaaagcc gccaagggta
     4321 tctttatacc gacttcaaaa aaattgtccc cggagcattt cagaaggcca acggaggatt
     4381 cctcgtcctt gacgccgaga aagtactgct caatttcatg tcgtgggaag cgctgaagcg
     4441 cattctccgc accggcgaag cgaccataga aaacctggga gagcagtacg gcgccgttcc
     4501 cgtctcttcc ctgcgccctc aacccatacc gataaacatg aaggtcgtga tggtgggaac
     4561 gccttttctt catgcgctgc ttcagtatta cgacccggag tttcagaaga tgttcaagat
     4621 caaggcggat ttcgacaccg acatggagcg aacgcctgag acagaaaaac agatggccca
     4681 gttcatcgct tcgtgcctta aaaaagaaaa cgggcttcct ttcgacgcat ccggagtcgc
     4741 cgaggtgata gactgttcgg ccagaatcgc cgacgatcaa gggcgaatct ccacggagtt
     4801 caacaaaatt acggagatca tcgtcgagtc cacagcctgg gctaaagcgg aaaatgccga
     4861 tactgttcaa cgtgaacacg tgaagaaggc tattcgagaa aagcgcttcc gttcggattt
     4921 aatccaagag aagatagcac gcgcctacca ggatggtctg gtcaggatcg acacttcggg
     4981 aaaagtagtc ggccagatca acggcctttc agtcatcgac ctgatggact atcgtttcgg
     5041 acacccctcc agaatcaccg ccaacgtctt tatgggacag gacggggtcg tcaatatcga
     5101 gagagaagtc aagatgacgg gacctatcca caataagggg ctcttgattc tgaccagcta
     5161 catggggcga aagtacgcgc aggatatgcc gctttcactc tccgctaaac tgacattcga
     5221 acagatgtac ggcgggatcg aaggagacag cgcttcctcg acggagcttt actgcctcct
     5281 ctcagccctc tccgaggttc cgctcaagca ggggatagcg gtaaccggat cggttgacca
     5341 gttcgggaac atccagccca tcggcggggt caacgaaaag atcgaaggtt tcttcgagta
     5401 ctgcgaacac ggcggtctca ccggagagca gggggtactc atccccgtgc agaacacacg
     5461 gcatctcatg ctcgacgaca cggtgatcga ggcagtcagc aaagggaaat tcttcatccg
     5521 cgcagtctcg acgatcgacg aagggattga ggcgcttacc ggagtttccg cagaatcgat
     5581 tcatcgcaag gtaaagaaca agctaaaaaa atgggcgaag aagacggaaa aactcaaaaa
     5641 aggcaaagac tccgaggcag gaggagacga tgactccgaa gaagaatagc aagaaagagc
     5701 ccccatccag gccgcaagat atcgacacgg ttttcgacat actggaagag ctgtggggaa
     5761 atgaaaagaa cgagccccgc ctgggacatg aagaaccctt agacgggctc attctcacac
     5821 ttctttccca aaacacgaat gatcgcaaca gggataaggg cttcgccgca ctgaaaaatt
     5881 cgttcccttc ttgggaactt gtggcatcgg catcggctga agctattgcc gatcgaatcc
     5941 gcccggctgg tcttgcaaag acaaaatcgg agagaatgct ccagatactc gcggcactca
     6001 aggaaagatt cggcgagtat tcgctcatgg cattgaaaga catggacaac gatgcagtac
     6061 gagagtatct ttcctcgctc cccgggatag gggcgaagac cgtggcgtgt gtgatgctct
     6121 tcgacttagg gatgtctgcc tttccggtcg acacgcatat cgccaggttc tgccggagaa
     6181 tgaagtgggc ggcggaatca ctccctcccg acgagataca gcctttgctt gaagaatggg
     6241 ttccagagag acgttttctg ggaggacatg tcaacattat tgaacacgga agagggctgt
     6301 gtcacgcccg tacccccgag tgtgggaagt gcccgttgca cagcaacaac ctatgcccgt
     6361 cggttcaaga ataaacaaat gtaaatcagc agaggagagc atttttcgag ctccctctgc
     6421 tggtttagaa aagagaagaa ggatcaggcc ttggaagatt tgtcttcggg attcgtcgct
     6481 tcaccggcct ctttcaaacg atcaagcaca attttgtacc cgtcggcgcc gtactcaagg
     6541 aactttttta ctctgctgat cgtcgcagtg cttgctcccg tttgctgcgc aatttgcgga
     6601 taggtgtacg cctcgctcaa caaccgggca acttcgagcc gctgagcaag agcgcggatc
     6661 tcgccaatgg tagcgacatc ttcaaggaaa cagtagacct catccggcgt ttctagagag
     6721 aggagggcgc ggcacagctg gtctgtcaac ttatccttcc atttttcagt catagcattc
     6781 tacctccttt gcattgtggc gaaaagagaa atgacggact ccatatcttc ctctgttcca
     6841 atacttacac gtaaccagca tgttgagtcc tctgtatcgg acgaaagagc gaagcgcttc
     6901 aagcacaaat ctccaagaga agaagcaaaa ctgctccagg gaaaaggagc tcggacaagt
     6961 acaaaattcg cgtcgctcga aaaggctctc cacccctgga ggcgattaac aagcgatata
     7021 aatccgtccc ggatgccgca aaggagttta actcttgcga gcatcacttc tgaatgctcc
     7081 agaataattt cggcggcagt tcccgaaaga atatttacgt tgaacggtga tcgggcagca
     7141 ttcagcgctg aagccgaatc ttttccgcaa acagcgtacc caagccggag ccccgcaagc
     7201 ccccacgctt tggaaagcgt tttcaataca acgacgtttg caggcaatac ctttcctcgc
     7261 atcgatgaaa gccagttatc ccgcgcgaac tccccgtacg cctcgtcgat cacaatggtt
     7321 gattcacaaa gcgcaactgc cttgccgata aaggaaacgg ggtgtgattg gcccgtcggg
     7381 ttgttcggag aatccacgag gaccagcgaa ggtttcagct ccgccattct gcttaaaaaa
     7441 agcgtatgat cgaagcgcgg ttcgtcaccg tttaagtctg cttgaacggt acataaactg
     7501 gctccgaaaa gatctgcgag gcgatagtac tctgaaaaag taggtgaaaa tacgagcacg
     7561 gaatctcctg gtttgacata acgggaaaaa agcatccaga gaatttcatc gccgccgttt
     7621 ccgggaacta tagagtcagc ggagaaacca caatacatgg agagtctctc ttttaaaact
     7681 gcatactccg ggtccggata tctattaaag gacgcgcgcc cgattttttg caggataagc
     7741 gccttaatct cttcagggag gtcgaacgga ttttcgtttt tatcaagcaa acggcgcaat
     7801 gggttcaccg cctccttcta ttttcagcaa gagaatcttt gaactacgac gtacagagga
     7861 gaataccata tcaatttcca ggaacatctg tactatgcca tctgcttcaa aacacaaaaa
     7921 ctctttctat gctcgaagcg cacttggact ttagcatgtt acaatgtatt tatttggcag
     7981 tctagcattc taccgcgtca atgtcaacga taagtgcgcg ttgtgtgcgt tgccagttca
     8041 tgtttcgcac tgcgcatcaa ggagggaaaa aattgaactc catcgaaatg attgtcgtcg
     8101 aacgtctttt tgaagaagaa gggattcttg ccggggatat tctttctcct gcgggagtcc
     8161 ttttgctccc ctcgggtctt gacctcgatt ctctgcgttc cacgcgtccg gacgca
//
LOCUS       DAOSWW010000013         8184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_5657_length_8184_cov_3.887809, whole genome shotgun sequence.
ACCESSION   DAOSWW010000013 DAOSWW010000000
VERSION     DAOSWW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5657_length_8184_cov_3.887809"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PK442_00555"
     CDS             complement(<1..763)
                     /locus_tag="PK442_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO86170.1"
                     /translation="MKLSIKVEPRLETPRMLGIAIPVISLAAALLSGAVFLFFMGVSP
                     FQAYSTIIRSSLGDAYGLSETLVKAIPLAMAAIAVMLSFTMLIWNIGAEGQIFMGAIA
                     AAAAVRYLFVDNHLAMFAIMFAAAGIAGGIWAAIAGYFRARWSVNEIITTLMLNYVAM
                     NLLNYFVYGPWRDPASLGFPMTAPFPDAARLPSFWGTRIHAGLFLAILFPLIFWVILR
                     FTRWGYEIRVMGENPKAAGFAGMSYIKNVVFVMFLS"
     gene            complement(760..2328)
                     /locus_tag="PK442_00560"
     CDS             complement(760..2328)
                     /locus_tag="PK442_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO86171.1"
                     /translation="MDARAPSKPLVRMEKITKRFTGVTANLDVDFDVRPGEVHALLGE
                     NGAGKSTLMNILYGLYRPDSGVLTIEGKDVSFSSPRDAIASGIGMVHQHFMLIPSQTV
                     WENMILGLEGLSSVLPKRAIRERILELSSRYGLSVDPDAKIWQLSIGEQQRVAILQML
                     YRRAKILVLDEPTAVLTPQEAEHLFVTVRQMTSEGHGIVFISHKMDEVMGLSHRVTIL
                     RKGRLVGTVDTASTTKEKLAEMMIGQKMIFSIEKKELQPGKPVIECASVCALGDRGIP
                     AVSRATFMIREQEILGVAGVAGNGQQELCETLVGLRKVESGAIRVNNEELTNAIPKTF
                     IDKGIHYIPADRKGTGLVPNMNVSENSILKRYWSRAVSKKGFIDWKEVFSFARGLVSR
                     YNVSTPSMETPVRNLSGGNLQKLMLGRELSDSPIAILAVHPTWGLDVAATKFVREQLL
                     AAREAGCAVLLVSEDLDELLAMSDRLAVMCKGEIMGILDSPAETPVEAIGLMMAGTPA
                     EGFHASAPRGGEKS"
     gene            complement(2496..3581)
                     /locus_tag="PK442_00565"
     CDS             complement(2496..3581)
                     /locus_tag="PK442_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO86172.1"
                     /translation="MKRFVVLLVFVLSVTLCGAAYAAFPAIPAEKINPGFVYIGPIGD
                     GGWTYMHEQGRLAMEKEFPGVKSTFVESIPEGPDAARVFETMIRNGSKVIFGTTFGYM
                     DFVQEVAKKYPDTIFMHCAGYKDAPNVGTYFGRMYQARYLSGLVAGKMTKKNRIGYVG
                     AHPIPEVIRGINAFTQGVRKANPEAEVQVIWIFSWLDPGKEKEAAKALIDSGVDILAM
                     HADTGATPQAAEEAGIYVIGYNNDMSAYAPTKHLTAPIWDWGMVYKETIGQVIKGTWQ
                     PSHIWYGLKEGMVALAPYGKDVPDLVKALVDVEKAKIVSGEWDVFHGPVKDQSGEVKV
                     PEGAAMTDGEMLSMNWFVEGVKGDIPK"
     gene            3756..4232
                     /gene="gpt"
                     /locus_tag="PK442_00570"
     CDS             3756..4232
                     /gene="gpt"
                     /locus_tag="PK442_00570"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HOO86173.1"
                     /translation="MSSERYHKTYPVSWEQVHRDCKALAWRLVSVCKWERIIGIARGG
                     LIPAAIIARELDVRLVDTVCVSSYVLRTQGEPSVLKEISGDGAGWLIIDDLVDTGKTA
                     QVVRRMLPKAHFAAVYAKPDGRAFVDTYITEVSQDTWILFPWDAETAYAKPIVEQR"
     gene            4383..4850
                     /locus_tag="PK442_00575"
     CDS             4383..4850
                     /locus_tag="PK442_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86174.1"
                     /translation="MTYLNYVFFLVFWGWILSVGYIRYILPFIESAFDTLEAMEKSGE
                     LFPRAIAFIAKLAMTGSQMYILGIWSAYCVLRTMIFLHEPGTNGWLYYITAFLVCEGF
                     LGAVAKKEKYRGLLSVFHSAMAMGLFVIFAMNPYFLRSVYPWLPPMMNFSFPH"
     gene            complement(4932..5315)
                     /locus_tag="PK442_00580"
     CDS             complement(4932..5315)
                     /locus_tag="PK442_00580"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO86175.1"
                     /translation="MADFVLLAGKISDIRLAEDPSHASGIEKRIVFAPGKFWNDYVAR
                     HFTVKGKNSKTPFHTHDWPHYVFILSGRCNATIDGTVYPLEKGCWAHVPPNVEHFFES
                     TGEEPLDFICIVPPKGDPAGSAHLA"
     gene            5629..6603
                     /locus_tag="PK442_00585"
     CDS             5629..6603
                     /locus_tag="PK442_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO86176.1"
                     /translation="MKRSIAVVSIVLLTLLFVASAAVAAVDIKLAHVVNENDAFHVCA
                     LKFKELVEAGSKGELTVTIYPNAQLGDERNLLESMRMGVVDSAIITGGPIMNFMPKFG
                     VFDLPFLFSTPEHAYKVLDGEIGKMMLNEMEQHGWKGLAYGERGFRNLTNSKQPVKTP
                     EDMKGLKIRLMQNPVYVDSFRALGANAIPMAWTEALTALQQGAIDGQENPLNVIVAFN
                     LFESQKHLAITRHAYAPNVIMMSKKTWEKLAPEMQSLVQDAAQKAAEHNRAYDNEKEA
                     EWLQFLKDKGMEVTETDTAAFREAVKPVYQKYEEQFGKELIEAILADK"
     gene            6684..7124
                     /locus_tag="PK442_00590"
     CDS             6684..7124
                     /locus_tag="PK442_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO86177.1"
                     /translation="MNRISEILLFAMMLAMIAVTTMQIVCRVFFEALIWSEELTTYLL
                     VAASLLGAAIAFKRGSHIAVTFLVHKLPLPLRKVTEFLVQGIGILFFFVVAYYGGVLM
                     KSEAMQTTPAMGISMTWIYLMYPVVGSIVLLHLVAGLGGILGRK"
     gene            7126..>8184
                     /locus_tag="PK442_00595"
     CDS             7126..>8184
                     /locus_tag="PK442_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO86178.1"
                     /translation="MGLVLAGSFAFFLLLGVPVGLAIGVSGLLIVFLNGFPLEMIPQT
                     MFSGNESFALVAVPFFVLAGDILAKGGISERIIAFAEATLGRFRGGLSIVSTVASMFI
                     AAISGSGAATTAAVGSALLPQLEEKGYDVDFSAALIAASGTIGVVIPPSVPMVLYAVI
                     AGVSVAKLFMAGFVPGFLMGLGLIVYSIYVARKRGYPASRSTTGREKWKLFLDATWGL
                     MTPVIILGGIFSGFFTPSEAAAVAVVYSLFVAFFIYRSLSFKQFYKIVVGSGVTSALI
                     MFIIATAKLFGWGLAFFQIPEAVARFLLGFAGGNTFMIYFMITIIILLAGMFMETASA
                     LIVLTPIFLPTIIRTGGNL"
BASE COUNT         1763 a         2176 c         2313 g         1932 t
ORIGIN      
        1 cggaaagaaa catcacgaac accacgtttt taatatagct catccctgca aaaccggcag
       61 ctttgggatt ttctcccatg acccgaatct cgtaccccca cctggtgaaa cggagaatca
      121 cccagaagat cagaggaaac aggattgcga ggaaaagccc tgcatggatt cgggtgcccc
      181 agaaagaggg gagacgagcc gcgtccggaa aaggggccgt catcgggaag ccgaggcttg
      241 cagggtcgcg ccacgggccg tagacaaaat agttcagcag gttcatggcc acataattga
      301 gcatcagcgt cgtgatgatc tcgttgacgc tccatcgcgc gcggaagtat ccggcgatgg
      361 ccgcccagat tccccctgcg attcccgctg ccgcaaacat gatggcgaac atcgccaggt
      421 ggttgtcgac gaagaggtag cgaacagcgg cggcggcggc aatagctccc atgaagatct
      481 gcccctccgc gccgatattc caaatgagca ttgtgaagga gagcatcacc gcgatcgcag
      541 ccattgcaag agggatcgcc ttgacgagag tctcgctgag accgtacgcg tcacccaaag
      601 aagagcggat gatggtggag taggcctgga acggagagac gcccatgaag aagagaaaaa
      661 cagctcccga aagaagcgcg gctgcgagtg agatgacggg aatggcaatg ccgagcatcc
      721 gcggggtttc aagtcggggt tccactttga tagagagttt catgattttt ctcctcctcg
      781 gggagcggag gcatgaaatc cttcagcggg tgttccggcc atcataaggc cgatggcttc
      841 gacaggcgtc tccgcaggcg aatccagaat gcccatgatc tcgcccttgc acataacggc
      901 aaggcggtcg ctcatggcga ggagttcgtc gagatcctcc gaaaccagca gaacggcgca
      961 tccagcctcg cgggcggcga gaagctgttc cctgacgaac ttcgtcgccg cgacgtcgag
     1021 tccccatgtg ggatgaacgg cgaggatggc tatcggggag tcgctcagtt cgcgtccgag
     1081 catcagcttc tggaggttcc ctccggagag gttgcgaaca ggagtttcca tcgaaggagt
     1141 gctcacattg tagcgggaaa ccagccctct ggcgaaagag aagacttctt tccagtcgat
     1201 gaatcccttc ttggaaacgg cgcggctcca gtatctcttc aggatggagt tctcgctcac
     1261 attcatattg ggaacgagcc cggttccttt ccggtcggcg gggatatagt gtatcccttt
     1321 gtcgatgaag gtctttggaa tcgcattggt cagctcctcg ttattgactc gaattgcacc
     1381 ggactcaacc tttcgaagtc ccaccagtgt ttcgcaaagc tcctgctggc cgtttcccgc
     1441 cactcctgcg acgcccagga tttcctgctc ccggatcatg aacgtcgcgc gcgatacggc
     1501 gggaatgccc cggtcgccga gggcgcagac ggaagcgcat tcaataaccg gtttcccggg
     1561 ctgaagctct ttcttctcaa tactgaagat catcttctgg ccgatcatca tctccgccag
     1621 cttttccttt gttgtggacg ccgtatcgac agttcctaca aggcggcctt tccgtaggat
     1681 cgtcacccga tgggaaagcc ccatgacctc atccattttg tgagagatga agacgatgcc
     1741 gtgtccttcg gatgtcatct ggcgtacagt cacgaagaga tgctccgcct cttgaggtgt
     1801 gagcaccgcc gtgggctcgt cgagcacaag gatttttgcc ctcctgtaga gcatctggag
     1861 aatcgccaca cgctgctgtt caccgatgga gagctgccag attttcgcgt cgggatcaac
     1921 cgacagtcca taccgggagg agagttcgag gatacgctcc cgaatcgcgc gtttagggag
     1981 gaccgaagag agtccttcaa gaccgagtat catgttctcc cataccgtct ggctcgggat
     2041 gagcatgaag tgctggtgaa ccatccctat tcccgaggcg atggcatccc gcggggaaga
     2101 aaaagaaacg tccttccctt ctatagtcag gacgccgctg tccggcctat agaggccgta
     2161 gagaatattc atcagggtcg atttgcccgc gccgttctcc ccgagaaggg catgcacttc
     2221 tcccgggcgg acgtcgaagt ccacgtcgag attggccgtc acccctgtaa agcgtttggt
     2281 gattttttcc atgcggacga gcggtttcga aggagctcgt gcatccatcc cttttccccc
     2341 ttgcgatcca gtgtacggat tctccgctca aaagagtgtc tgtggagagg atgtgtatcc
     2401 gttctggatc ggtaatgtag tgatgaatcg ggaagaaaaa ggggcgcgga aattcgcgcc
     2461 ccgaagttcc cccatatatg atacaaaaga aagggctatt tgggtatatc tcccttgacg
     2521 ccttctacaa accagttcat ggaaagcatc tctccgtcgg tcattgcagc gccttcagga
     2581 accttcactt cgccgctctg atccttcacc gggccgtgga atacatccca ctctcccgac
     2641 acaatcttgg ctttctcgac gtcgacgaga gctttcacca agtcgggtac atcctttccg
     2701 taaggagcga gagccaccat gccctctttc agtccgtacc agatgtggct gggctgccat
     2761 gtccccttga tgacctggcc gatcgtctcc ttgtacacca ttccccaatc ccatatgggc
     2821 gcggtgaggt gcttcgtcgg cgcataggcg ctcatgtcgt tattatatcc gatgacatag
     2881 atacccgcct cttcagccgc ctgcggggtc gcgccggtat cggcatgcat cgcgaggata
     2941 tcgactccgg agtcgatgag cgcttttgcg gcttcctttt ccttgcccgg gtcaagccag
     3001 gagaaaatcc agattacctg aacttcggcc tcggggttcg ccttgcggac gccctgcgtg
     3061 aacgcgttga ttcccctgat gacctcgggg atggggtgtg cgccgacgta gccgatacgg
     3121 tttttcttgg tcattttccc tgcgacgagg ccggagagat aacgagcctg gtacattctg
     3181 ccgaaatatg ttccgacgtt cggggcgtct ttgtatcccg cgcagtgcat gaaaatggtg
     3241 tcagggtact tcttcgcgac ctcctggacg aaatccatgt agccgaaagt cgtgccgaaa
     3301 atgaccttcg agccgttgcg tatcatggtc tcgaagactc tggccgcgtc ggggccttcc
     3361 ggaatggatt cgacaaaggt cgacttcacc ccggggaatt ccttctccat ggcaaggcgg
     3421 ccctgctcat gcatgtaggt ccatccgccg tcgccgatgg ggcctatgta gacaaatccc
     3481 gggttgatct tctcggcggg gatcgcgggg aacgcggcgt acgccgcacc gcacagagtg
     3541 acgctcaaaa caaaaacgag gagcacaacg aatcttttca cgctttcaac ctccctgaat
     3601 ataagaattg aggggtacgc ccctccttag atattataca gtagcgtctt aagaaaaaaa
     3661 atgtagagga agcattttac tttttcagaa ggaaacgggc atgctacaat acaccagtcg
     3721 gctgcgcgag aagaattgaa ggaggagtgg gtggcgtgtc aagtgaaaga tatcataaga
     3781 cctatccggt ttcatgggag caggtgcatc gcgattgcaa agccttggcg tggcgtcttg
     3841 tcagtgtgtg caagtgggaa agaatcatag gaattgcgag gggcggactg atccccgcag
     3901 caatcatcgc ccgggagctt gatgttcggc ttgttgatac agtctgtgta tcgagctacg
     3961 tgctgcgcac acagggggag ccttcggttt taaaggaaat ctccggcgac ggggcagggt
     4021 ggcttatcat cgacgatctc gtcgatacgg gaaaaaccgc ccaggttgtt cgacgaatgc
     4081 tcccgaaagc ccactttgcc gctgtgtacg caaaaccgga cggccgtgcg ttcgtggata
     4141 cctatataac ggaagtcagt caggacacgt ggatcctctt tccgtgggat gcggaaaccg
     4201 cctacgcgaa accgatagtg gaacagaggt agcctgcctt gctcacggcc gcctgaacaa
     4261 acgctctgga aaaaagaggc gggttggagg gtgcgccgtt tcatttcgtg tctcgcagag
     4321 tacagggaag cagtatattg cgagcgcttt agttatcata cgctccggaa ggcgggattt
     4381 tgttgaccta tcttaattat gttttttttc ttgttttttg gggatggatt ctctccgtcg
     4441 gatatattcg ttatatcctt cccttcatcg aatcggcttt cgatacgctt gaagctatgg
     4501 aaaagagcgg cgagcttttt ccgagagcaa tcgcctttat tgcgaagctg gcaatgacag
     4561 gaagccagat gtatattctg ggtatttggt ccgcctactg cgtactgagg acgatgattt
     4621 ttcttcacga accgggaaca aacggttggc tgtattacat aacggcgttc cttgtctgcg
     4681 aagggtttct tggcgctgtc gccaagaagg agaaatacag ggggttgctt tcagtgttcc
     4741 attccgcaat ggcgatgggg ttgtttgtca tttttgctat gaacccgtac tttcttcggt
     4801 cggtgtaccc gtggcttcct ccgatgatga acttttcctt tccgcattaa aaagagagag
     4861 gccggcaagc gacgcttccg gcctctctct gctttggggg atgctgtaaa aaggctgcct
     4921 cccgccgaaa atcaggcgag gtgggcgctt cctgcggggt cgcccttggg aggcacaatg
     4981 cagataaagt ccaacggctc ttcccctgta ctctcgaaaa agtgttccac attcggagga
     5041 acatgagccc agcacccttt ttcaagcgga tagaccgtgc cgtcaattgt ggcgttgcat
     5101 cttcccgata aaataaacac gtaatggggc cagtcgtgcg tgtggaaagg cgttttcgag
     5161 tttttgccct ttacggtaaa gtggcgcgca acgtagtcgt tccagaattt cccgggggcg
     5221 aagacaattc gtttctcgat acccgatgca tgcgatgggt cctccgccag ccggatgtcg
     5281 gaaattttcc ctgcgagcag cacaaaatcg gccattgtat aaccactctc cttacgtatt
     5341 gtcctcaatg tgccagagga acgccggtct gttccgatga tagcaggcaa gcgaagcgtt
     5401 gtccagaatc acaaacagaa tcacaaacat atgtgcggcg aagactgtag cgggtttcct
     5461 taaatgaaca ttcggtttac tggacgcaaa acattgaaac agacttgcaa ttctcgcgct
     5521 aatagctata atgcgttgcc aaggcttaat gctcctcttt ggatgctgtg tccgaggagc
     5581 ggttttttca caatcatatc ttcaatacaa ccaggagggg atagtatcat gaagagaagt
     5641 atcgcagttg tatcgatcgt ccttttgacc ttgctctttg tcgcttccgc cgcggtcgcg
     5701 gcggtggata tcaagcttgc gcacgttgtg aatgagaatg acgcgttcca tgtctgcgcg
     5761 ctgaagttca aggagcttgt cgaggcggga agcaaagggg agctcacggt gacgatatac
     5821 cccaacgcgc agctcggcga cgagcggaat ctcctcgaaa gcatgcgcat gggcgtggtt
     5881 gattccgcca tcatcaccgg cggtccgatc atgaacttca tgccgaagtt cggtgttttc
     5941 gatctgccat tcctcttctc gacaccggag catgcgtaca aagtgcttga cggcgagatc
     6001 ggcaaaatga tgctcaatga gatggagcag cacggctgga aggggctggc gtacggcgag
     6061 cgcggcttcc gtaatctgac gaacagcaaa cagcctgtca agacccccga ggatatgaag
     6121 gggctgaaga tccggctcat gcagaacccg gtctatgtcg actccttcag agcgcttggc
     6181 gcgaacgcga tccctatggc gtggaccgag gcgcttaccg cactccagca gggagctatc
     6241 gacggacagg agaatccgct gaacgtcatc gttgccttca atcttttcga gtcccagaaa
     6301 catcttgcga tcacccgcca tgcctacgcg ccgaacgtga ttatgatgag caaaaagacg
     6361 tgggaaaaac ttgcccccga gatgcagtcc cttgttcagg atgcggcgca gaaggcagcg
     6421 gagcataaca gagcctacga caatgagaaa gaggccgagt ggctccagtt cctgaaggat
     6481 aagggaatgg aagttacaga gaccgacacg gccgcgttcc gcgaggctgt caaacctgtg
     6541 taccaaaagt acgaagagca gttcggtaaa gagcttatcg aggctattct ggctgacaag
     6601 taaggctgta gcggatgaat tccagagata acgggacaag aggcgggagc gtattctcgc
     6661 ctcttgtccg attaagcgat tttgtgaata ggatcagcga aatacttctc ttcgcgatga
     6721 tgctcgcgat gattgccgtg acgacaatgc agattgtctg cagggtcttc ttcgaggcgc
     6781 tgatctggtc ggaggaactg acgacatatc ttctggtcgc cgcgtcgctc ctgggcgccg
     6841 cgatagcttt caaacgaggc tctcacatag cggtaacgtt ccttgtacac aagctccctc
     6901 ttccgctgag aaaagtgacg gaattccttg tgcaggggat agggattctc ttcttcttcg
     6961 tcgtcgcgta ctacggcggc gttcttatga agtccgaggc gatgcagaca actccagcca
     7021 tgggtatttc catgacatgg atttatctga tgtacccggt cgtggggagt atcgttctgc
     7081 tgcatcttgt cgctggactc ggcggcattc tcgggaggaa gtaatatggg actcgttctt
     7141 gcaggcagtt ttgcgttttt tttactcctg ggggttccgg tgggacttgc catcggcgta
     7201 tcgggtctgc ttatcgtctt tttgaacgga ttccctcttg agatgattcc gcagaccatg
     7261 ttctccggga atgaatcgtt tgcgctcgtc gcggtccctt ttttcgttct tgcaggagat
     7321 attctcgcga agggcggcat ctcggagcgg attatcgctt ttgccgaggc gactctcgga
     7381 aggttccgag gcggactctc catcgtctct actgtggcct ccatgttcat cgcggccatc
     7441 tcaggctccg gagcggctac gacggcggcc gtcggctccg cgctcctccc gcagcttgag
     7501 gagaaggggt acgatgtgga cttctcggcc gccctgatcg cagcatccgg gacgatcggc
     7561 gtggtcattc cgccaagcgt cccgatggtg ctctatgcag ttatcgccgg agtttccgtg
     7621 gcgaagctct tcatggcggg attcgtcccg ggttttctga tggggctcgg acttatcgtc
     7681 tattcgatct acgtcgcgag aaagagagga taccccgcga gcagaagcac cacgggccga
     7741 gagaagtgga aactcttcct tgacgccaca tggggtctta tgacgcccgt catcattctc
     7801 ggaggcatct tctcggggtt cttcacccct tcggaggctg ctgccgtggc ggtggtctac
     7861 tcgctcttcg tcgcattttt catctacaga tcgctgagct tcaagcagtt ctacaagatc
     7921 gttgtcggtt cgggcgtcac ctcggcgctc atcatgttca tcatcgccac cgcgaagctc
     7981 tttggatggg ggctcgcctt ctttcagata ccggaggcgg tcgcgcgttt cctcctcggt
     8041 tttgccggcg ggaatacctt catgatctac tttatgatca cgatcatcat tcttcttgcc
     8101 gggatgttta tggagactgc ttccgcactg atcgtcctca cacccatctt cttaccgacg
     8161 atcatccgca ccgggggaaa cctt
//
LOCUS       DAOSWW010000014         8014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_5793_length_8014_cov_3.775223, whole genome shotgun sequence.
ACCESSION   DAOSWW010000014 DAOSWW010000000
VERSION     DAOSWW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5793_length_8014_cov_3.775223"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PK442_00600"
     CDS             complement(<1..1309)
                     /locus_tag="PK442_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86179.1"
                     /translation="MNIPRLVLAEERREGKVAQSILVASVLKSLGYPLRLFCAGVDEH
                     LVRFLKLVTEQDVTVLDPFSCGSMRNFKLLFQRAASPDTLNIIFGSIGERRDENTFPV
                     HPLPGEIARILECPLVTMLYADSSAAVTSRILENVFNQISAQGVITSAVIFSSVLNPR
                     EYQLLETEAGRRVPLLCLGFIPKTLEKNNPPLMELCLQDMAQRAAFPVKAATAQLLGM
                     VDQVDWNVLWGIAKQCKECVAMDDPFKGKASGISVGILSFPSFELEGNNAALLFKHLG
                     CTVYPIPLDSALLRRDLNALYVPHGPGFLCAERLLGKSGVREWFTSIFHARKAVLVNG
                     GITPLLGESFSLPNRKEYEGLKFFRFKGKFDIPSSDDVRRVEAVSTDGDVMLHQGDKI
                     RGYLPPYISVLNPGNVAGAVWSLQDTVKNMEIGLSGWSRGAALA"
     gene            1405..2706
                     /gene="asnS"
                     /locus_tag="PK442_00605"
     CDS             1405..2706
                     /gene="asnS"
                     /locus_tag="PK442_00605"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOO86180.1"
                     /translation="MTALWVSIKDLPSHVEETVVIRGWLYNLRSSGKIHFLQLRDGSG
                     TVQGVMVKKEVSEDLFAAAKGLWLEASVEVTGTVRKDERAPSGVELSVTDLRVFQNPS
                     EEYPIGKKDHGIDFLLDHRHLWLRSSRQQAIMRIRDRVIRSAREYLQENGFLLIDSPI
                     ISGAAGEGVSGLFEIDYFDSKAYLAQTGQLYAEAAAAAFGKVYCFGPTFRAEKSKTRR
                     HLTEFWMLEPEVAFYDHKDNMALQEGLICHIVAKVLEHCRTELASLERDVSKLENIKA
                     PFYHLQYDEAIEMLHKLGSDTPYGDDLGNEDETILTQQFDRPVFIECYPKTVKAFYMK
                     EHPDNPNLVLCDDLLAPEGYGEIIGGSQREDDYDRLIARIREQGLPEEPYEWYLDLRK
                     FGTFVHSGFGMGIERVVAWISGLQHIREAIPWPRTIYRLKP"
     gene            2744..4477
                     /locus_tag="PK442_00610"
     CDS             2744..4477
                     /locus_tag="PK442_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOO86181.1"
                     /translation="MIYSDFDPKPTFRQFKRLIGDGEVGGKARGIAFAFNAVTGSPLE
                     SVVDFPDVNFVVTTEGFDEFVSDNGIETLLREHAASEEENCDEDTSQELFDRLSAVFQ
                     SGRIRSSLLQDLEGALESIGDFPLAIRSSSILEDSRKLSFAGKYGTRFSANIGSVEDR
                     RDLLANALKDVWASLYNPAARAYRKKHGLVDSDESMAVVIQPVIGRRHNGMYYPEIAG
                     TAFSKVYRRPSTRIKKENGVMRFCFGLGTRTVDRLKANISYLSHPMLRPQGNLPADIA
                     MTSQSEFDYIDRTSGTFMTGLLSEHISFILKEHKLASSFIEIFSDNVLYWAGSDQLSN
                     GKPIFSFSNFPRRHPKFFNIVKDLSAFLEEKMGMPADMEFTYDTESEKLTLLQLRPLA
                     SYEEMARVEIPEVREEKIILMGNKMVSNGKMQNIHHLVFVDPELYGKDATFHEVAREI
                     GRINHKLSGTDYILVGPGRWGSTNPKLGVPVRYNEICNCGCLVEVGILESDYTPELSY
                     GTHFFLDLDVDGTLYLPVFDGMKGNLFNRTWLDSSFYERKRHPAVRHYTGDFSVFMDG
                     EKEIGAVISND"
     gene            4585..6180
                     /locus_tag="PK442_00615"
     CDS             4585..6180
                     /locus_tag="PK442_00615"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOO86182.1"
                     /translation="MKHFFSRITVLLLLLVISSDPSYGCTGILVGREASEDGSVLNSQ
                     TADGWYDSNLRVVPGETFPEGEDVSVYFGLLGDEPLPPFELGRIPQASTTYAYFRTAY
                     SCFNEHQLSIGESTIGQKDMLKAFPGEGGALLTVEQLMILALQRCKTSREAVLLIGEL
                     AERYGFLGSCANEGESLSIADPYEAWIMELLSVGFDWKPGSAPGAIWVARRVPDSHVA
                     ILCNASRISLVDENSEDFLFSKGYKEPALRHGWYDPESGAPFNWRKTYAPESGPWSPS
                     SMWVRGRLHAIHKRLLPSRSWDPYAETDSYPFSFAPEKPLSVKGIMEIFRSTMEGTPF
                     CMEDNPAWYVPRGNRELVRSAKATPFPDRFMRDLLNIPYTRPVAAKTSYSFITQSRSW
                     LPSPLGGVLWYSLDTPHFSTYAPVYAGVRSVPESWSNFKKDGFTLDSARWAVLLSSSL
                     TNSNYQRSMPTLKSLRDPLEKKTFHDFLQWEKEGSHLLLHSSPELQEWMDAITAKKME
                     EIHQTYRSIAQALIMEATIIDLW"
     gene            6167..>8014
                     /locus_tag="PK442_00620"
     CDS             6167..>8014
                     /locus_tag="PK442_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO86183.1"
                     /translation="MICGKRTAVRSLSILGLFLLLSVRTVYAEQAMLVGSDAGRTYHV
                     LCLDAGAPYIFRAENGDLQGVYVDILSALGEREGLKLSLSLGDTHDARRGIHLSRIDF
                     IVGTPYPVPADSELFTFFSVKNMDTADTRLIDDFELMKHRLSGKEFASYCFSLPFFEE
                     PYTLFAAGSEALPLSSLRGKILLVIEDSPEERHIRASAFNVKILQEDTMDSAVHSLLA
                     NKGDAALLGAYQGIRHTQNLPQKGRITPSRPFFFSLGKGITVMKGQADLALAMMERLS
                     AMKQSGEISKITGAWLSQYDPPVLSRQEIWNIVGGGLLALFAALLWNLLLKRKVGIMV
                     KEREKILDFIKDGILAVDNGGRITMLNRSAQNLLGLSSDSIGKEADLCIPGLAVGIVL
                     TDGRPVYNLQQNLNGALVSCNKVPILEDSHSSGVIITLRDMSELQAMAEEITGVRTYV
                     ETLRVQGHEFMNKLQTISGLIQLQRYDRAIEFIASETDSSLSAQAFLAERIKNAAVCG
                     IIMGKAGRCRELGIAFILDPESFCCDHGESINDRSLVIIIGNLLQNAIEAVVETGVTP
                     DARIEFSIFDESGQILIDVFDNAGIMTEEAASRLFEKGFTTKYKAEPSGF"
BASE COUNT         1940 a         2165 c         2037 g         1872 t
ORIGIN      
        1 tagctaaagc ggctcccctg ctccatcccg agagcccgat ctccatattt ttgaccgtgt
       61 cctggagact ccacactgct ccggccacat tccccggatt cagcacagat atgtaggggg
      121 ggaggtaccc ccggatttta tctccttgat ggagcataac atctccgtcg gtagaaacag
      181 cctcaaccct gcggacgtca tcggatgaag ggatatcgaa ctttccctta aaacggaaga
      241 acttgagccc ctcgtactct tttctgttcg gtaaagaaaa cgactcccca agaagcggcg
      301 taatgccgcc attaaccagc actgccttcc gggcgtgaaa aatcgatgta aaccattccc
      361 gaaccccgga cttccccaat agtcgttcag cacaaaggaa ccccgggccg tgcggaacgt
      421 aaagtgcgtt caagtcccgg cgaagaagcg cactgtcaag gggaatcggg tagaccgtgc
      481 acccgaggtg cttgaaaagc aacgcagcgt tatttccttc cagctcaaac gagggaaaag
      541 aaagaattcc aacgcttatg ccgctggctt ttcccttgaa aggatcatcc atagctacgc
      601 actctttaca ctgttttgct atcccccata ggacattcca gtcaacctgg tcgaccatac
      661 ccagcagttg cgcagtcgcg gcttttacag ggaacgccgc tcgctgggcc atatcctgaa
      721 gacagagttc catgagcggc gggttgttct tttcaagcgt tttcggaata aaccccaggc
      781 aaagtagagg aacgcgacgt cccgcttcgg tctccaacag ctgatattcc cgaggattca
      841 agacagagga gaagatcact gcggaagtaa tcactccctg tgctgatatc tggttgaaaa
      901 cattttcgag aattcttgag gtgactgcgg ctgaactatc cgcatagagc atcgtcacca
      961 gcgggcattc aaggatacgt gcaatctcgc cggggagagg gtgtacagga aaggtgtttt
     1021 catcccgacg ttccccgatt gaaccgaaga tgatgttcaa ggtatccggc gacgcagcgc
     1081 gttggaagag gagtttaaaa ttccgcatgc ttccgcatga aaaaggatcg agtaccgtta
     1141 cgtcttgctc ggtgacaagt tttaaaaaac gaacaagatg ctcgtcaact cctgcgcaaa
     1201 acaaacgaag agggtatccg agacttttca ggactgacgc gacgagaata ctctgggcaa
     1261 ctttaccctc tcttctttct tccgcgagga caagacgtgg aatattcact ggttcctctc
     1321 ctttttttta aagtatagtg tatcatatac cgcatggaat ttgataatca cttatcgttc
     1381 aagacgtctg gaggttgatg acgcatgaca gcattatggg tttccatcaa agatcttcct
     1441 tctcatgtgg aggagactgt cgttatccgc ggatggcttt acaacctgag gagttcaggg
     1501 aagattcact ttcttcagct acgcgacgga tcgggcacag tacagggagt catggtaaaa
     1561 aaagaagttt ccgaagatct ctttgccgcg gctaaggggt tgtggctgga ggcatccgtt
     1621 gaggttacgg gcactgttcg aaaagatgaa cgcgcccctt ccggcgttga actttcggtg
     1681 acggatctgc gtgttttcca gaatccttct gaggaatacc ccatcggaaa gaaggaccac
     1741 ggtatcgatt tccttctcga ccatcgtcac ctctggctca gaagcagccg tcagcaggcg
     1801 atcatgcgga ttagagaccg tgttatccgt tcggcaaggg aatacctcca ggaaaacggc
     1861 tttttgctga ttgacagccc gatcatcagc ggcgccgccg gagaaggagt ttccggcctt
     1921 ttcgagatcg actacttcga ctcgaaggcc tacctggccc aaaccggaca gctctacgcc
     1981 gaggccgcgg cggcggcgtt cggaaaggtt tattgcttcg gccctacgtt ccgcgcggaa
     2041 aagtcaaaga cgcgccgcca tctcacagag ttctggatgc tcgaaccgga agtcgccttc
     2101 tatgaccaca aagacaacat ggcgcttcag gaagggctga tatgtcacat tgtcgcaaag
     2161 gtactcgaac attgcagaac ggagctcgcc tcgcttgaac gcgacgtctc caagcttgag
     2221 aatataaagg cgccgttcta tcatcttcaa tacgacgaag ccatagaaat gctgcacaaa
     2281 ctcgggagcg acacccctta cggcgacgac ctcggcaacg aagacgagac gatactcacc
     2341 cagcagttcg acagacccgt ctttatagaa tgctatccaa agactgtgaa ggcattctat
     2401 atgaaagagc accccgacaa cccgaacctt gtcctctgcg acgaccttct tgcccccgag
     2461 gggtacggcg agatcatcgg cggttctcaa agagaggacg attatgatag acttatcgca
     2521 agaatcaggg agcaagggct tcctgaagaa ccctacgaat ggtatcttga cctcaggaaa
     2581 ttcggcacgt tcgttcacag cggattcgga atgggaatag agcgcgtcgt ggcgtggatt
     2641 agcggattgc agcacatccg tgaggctatt ccatggcctc gcaccattta ccgtttgaag
     2701 ccctgacgtt cgttattttc ccatttcggg gaggcatact caggtgattt actcggactt
     2761 tgacccaaaa ccaacgttcc gccagttcaa acggctgatc ggcgacggcg aagtaggagg
     2821 aaaggcgaga ggaatcgcct tcgctttcaa cgctgttacc ggaagccctt tagagtctgt
     2881 cgtggatttt ccggatgtga atttcgtcgt gacaacggaa ggtttcgacg agtttgtcag
     2941 cgataacgga atagaaaccc ttttgcgcga acatgctgct tcagaagagg agaactgcga
     3001 cgaagacaca tcccaggagc ttttcgacag actttccgcc gttttccagt ccggcagaat
     3061 acgttcttcc ctgttgcagg atcttgaagg cgcgcttgaa tcgatcgggg atttcccact
     3121 cgcgatccgc tcaagctcca ttctggagga ctcgcgcaag ctctccttcg ccggaaagta
     3181 cggcacacgt ttttcggcga acatcggcag cgtggaagat cgcagagacc ttctcgccaa
     3241 tgcgctcaag gatgtttggg cttcactgta caatcctgcc gcgcgggcct acagaaagaa
     3301 acacggactc gtagactccg acgaatccat ggctgtcgtt attcagcctg tgataggacg
     3361 ccggcataat gggatgtact atcctgaaat tgccggaaca gccttttcca aagtgtaccg
     3421 gcgtccttca acgaggatca aaaaggaaaa cggggtcatg cgattctgct tcggtctcgg
     3481 gacgcggaca gtggaccgcc tgaaggcaaa tatctcctat ctctcccacc cgatgctccg
     3541 gccgcaggga aacctgccgg cggatattgc gatgacaagt cagagcgagt tcgattacat
     3601 cgacagaacg tccggaacct ttatgaccgg tcttctctct gagcatattt ctttcatcct
     3661 gaaagaacac aagctcgctt cttccttcat cgagatcttc tccgacaacg tactctactg
     3721 ggcaggttcg gaccagttga gcaacggaaa gcctattttc tctttttcaa attttccaag
     3781 aaggcacccg aagttcttca atatcgtgaa ggacctttcc gccttcctcg aagaaaagat
     3841 gggcatgccc gccgacatgg aattcaccta cgatacggaa agcgaaaagc tgacgcttct
     3901 ccagctccgg ccccttgcct cttacgaaga gatggctcgt gtggagatac ccgaagtccg
     3961 cgaagaaaag attattctta tggggaacaa aatggtgagc aacgggaaaa tgcaaaatat
     4021 tcaccatctc gtctttgtgg atcctgaatt gtacggcaaa gacgccactt tccacgaagt
     4081 ggcgagagag atcggacgca tcaatcacaa gctctccgga accgactaca ttcttgtcgg
     4141 gcccggaaga tggggaagca ccaaccccaa acttggcgtc ccggtacgct ataacgaaat
     4201 ctgcaactgc ggttgccttg tggaggtcgg tatcctggag agcgattaca ccccggaact
     4261 ttcctacgga acgcattttt tcctggatct tgatgtcgac ggaacgctct accttccagt
     4321 atttgatggt atgaaaggaa atcttttcaa ccggacctgg ctggattctt cgttctacga
     4381 acgcaaacgc catcctgcgg tgcggcacta cacaggggac ttctccgtct tcatggacgg
     4441 agaaaaagag atcggcgcag ttatttcaaa cgattaaccc acccggccgt ttcgccattt
     4501 tctgcggaac ggccggccat tccgtatctc ttcttctact cccaatgact gcactgacga
     4561 actatgtaga ggagtcgctt ccaaatgaaa catttttttt ccagaatcac cgtattgctc
     4621 ctacttttgg tcatcagttc ggatccttcc tacggatgca ctggcatcct cgtcgggcgc
     4681 gaagcaagcg aagacggttc cgttctcaac tcacaaaccg cagacggatg gtacgattcc
     4741 aatcttcggg ttgttccggg ggaaaccttc ccggaagggg aggatgtatc agtctacttc
     4801 ggactgctcg gcgacgagcc tcttcccccc tttgaactgg ggcgtatacc ccaggcatct
     4861 acaacctacg cttatttcag gaccgcctac tcatgtttca atgagcacca gctctctata
     4921 ggagaatcca ccattggcca gaaggacatg ctcaaagcct ttccgggaga aggaggggct
     4981 cttctcaccg tcgaacagct catgattctg gcccttcagc ggtgtaaaac atcccgtgaa
     5041 gccgttctcc tcatcgggga gcttgccgag cgatacggtt ttctcggttc atgcgccaat
     5101 gaaggcgagt cgctttccat cgcggatccg tatgaagcgt ggatcatgga gctgctgagc
     5161 gtcggatttg actggaaacc aggctccgct cccggcgcaa tctgggttgc cagaagagtg
     5221 ccggattctc atgtggctat tctgtgcaac gcaagccgta tctccctagt tgatgagaac
     5281 agcgaggatt ttcttttttc aaaagggtac aaggaacccg ctctccgtca cggctggtat
     5341 gatccggaat ccggcgcccc cttcaattgg agaaagacct atgcgccgga atccggcccc
     5401 tggtcgcctt cttcgatgtg ggtccgaggg cgcttgcacg ctattcataa gcgtcttctt
     5461 ccttcaagaa gctgggaccc ctacgctgag accgactcct atccgttttc tttcgctcct
     5521 gaaaaaccgc tctccgtaaa agggataatg gagatctttc gctcgactat ggagggcacc
     5581 ccgttctgta tggaggacaa ccccgcatgg tatgtcccta gaggaaaccg ggagcttgtc
     5641 cgcagtgcaa aagccactcc tttccccgac aggttcatgc gcgacctctt gaatattcct
     5701 tacacacgcc cggtcgcggc aaaaacgtcc tacagcttta tcacacaatc ccgttcatgg
     5761 cttccatctc cgctcggagg cgttctctgg tattcactcg acaccccgca tttcagcaca
     5821 tacgcccccg tgtacgccgg agtgcgcagt gtgccggaaa gctggtcgaa cttcaaaaaa
     5881 gatggattca ctcttgattc ggcgcggtgg gcggtgcttc tttcgtcatc acttaccaac
     5941 agcaactatc agaggtcgat gccgacgttg aaatcactgc gcgatcctct ggaaaaaaag
     6001 acgttccacg actttctcca atgggaaaag gagggttccc atttgctcct tcactcctca
     6061 cctgaacttc aggaatggat ggatgccatc accgcgaaaa agatggagga aatacaccaa
     6121 acgtacagga gcattgcgca ggctcttatc atggaggcaa caattattga tctgtggtaa
     6181 aagaacggcc gttcgttcac tctcgattct cggacttttt ctcctgctct ccgtccgtac
     6241 cgtatacgcc gaacaggcga tgttggtagg aagcgacgcg gggcgcacgt accatgtctt
     6301 gtgcctggac gcaggagccc cctatatctt cagggcggag aacggagatc ttcaaggagt
     6361 ctatgtggac atactgtccg ctctggggga gagagaaggg ttgaagctct ccctctccct
     6421 aggcgataca cacgatgccc gccggggcat ccacctctcc cggattgatt tcatcgtagg
     6481 aaccccctac cctgttcccg cggactcgga actttttact tttttttcgg tgaagaacat
     6541 ggacacagcg gacactcgcc ttatcgatga ctttgaactg atgaaacatc gcctttccgg
     6601 caaggagttt gcttcttact gtttttctct cccttttttc gaagagccgt acacgctctt
     6661 tgccgccggg agcgaagccc tcccccttag cagcttgaga gggaaaatac tgctggtcat
     6721 cgaagattct cctgaagaac gtcacatcag ggcaagcgcg ttcaatgtca agattcttca
     6781 agaggacacc atggattccg cggttcattc tctgctcgcg aacaaggggg atgcggcgct
     6841 tcttggagca taccagggca ttcggcacac gcagaacctt ccccaaaagg ggaggatcac
     6901 cccctcccgc ccgttcttct tttcgctcgg caaagggatc acggttatga aagggcaggc
     6961 tgacctggct cttgcaatga tggaaaggct ttcggccatg aaacagtcgg gagaaatctc
     7021 gaagataacg ggcgcgtggc tctctcagta cgatccgccc gttttaagca gacaggagat
     7081 atggaacata gtcggaggag gccttttagc gctctttgcc gcgctcttat ggaatttact
     7141 gctgaagcgg aaggtcggga ttatggtcaa agagcgcgag aaaattctcg attttatcaa
     7201 agatggtatt cttgctgtgg ataatggcgg acggattacg atgctcaacc gctccgcaca
     7261 aaatctcctc ggtctctcct ccgactctat cggcaaagaa gccgatctct gtattcccgg
     7321 tcttgccgtc ggcattgtgc tgacagatgg acggcctgtc tacaaccttc aacagaatct
     7381 caacggggcg ctcgtttctt gtaacaaagt tcccatactg gaggattctc actccagcgg
     7441 cgttatcatt acacttcgtg atatgtccga gcttcaggca atggcggaag agattaccgg
     7501 cgtccggacc tatgtagaaa ccctgagggt acagggccat gagttcatga acaagttgca
     7561 gacaatttcc ggactgattc agctgcaacg ctacgacagg gccatcgaat tcattgcatc
     7621 cgaaacggac tcaagtctct cggcgcaggc attcctggcc gagagaataa agaacgccgc
     7681 cgtctgcgga atcatcatgg gcaaagcggg acgctgccgt gaactgggaa tagcctttat
     7741 actcgaccct gagagctttt gctgtgacca cggagagagc atcaacgacc ggtctctggt
     7801 gatcatcatt ggaaatcttt tacaaaatgc gatagaagcc gtcgttgaaa ccggagtaac
     7861 tcccgatgcg cgaatcgagt tttccatctt cgacgaatcg ggacagatac tgatagacgt
     7921 cttcgataac gccggaatca tgactgaaga agccgcatcc aggcttttcg aaaaagggtt
     7981 cacaacgaaa tacaaagccg agccctccgg tttc
//
LOCUS       DAOSWW010000015         7757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_6042_length_7757_cov_3.825370, whole genome shotgun sequence.
ACCESSION   DAOSWW010000015 DAOSWW010000000
VERSION     DAOSWW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_7757_cov_3.825370"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PK442_00625"
     CDS             complement(<1..567)
                     /locus_tag="PK442_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOO86184.1"
                     /translation="MAESTIRRDPEEVRSRVVFLREEILRHEHLYYVLDTPEIPDDQY
                     DGLFAELRRLEEEHPSLRSPDSPTQRVGGKAAEGFAKVRLSVPMLSLDNALDTEELSA
                     FLTRTAPFAGEHGYLCELKIDGLAVSLVYEDGVFAQGTTRGDGRTGENVTANIRTIRN
                     LPLRLSGPFPQKMEVRGAVLLQRQHFAEL"
     gene            complement(587..2137)
                     /gene="lysS"
                     /locus_tag="PK442_00630"
     CDS             complement(587..2137)
                     /gene="lysS"
                     /locus_tag="PK442_00630"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOO86185.1"
                     /translation="MNTVPGGDESGPGDEIVRQRMDKLERLLSEEGYNPYILEKWERK
                     HQLEYVRRNFDHLEADEIDESVSIVTAGRIMTLRKHGKAMFANLADETETMQLYLQFN
                     AMGDEQYTFAKKWVDTGDWVGIVGYPFRTQRGELTIHVRQCVLLCKALRPLPEKWHGL
                     KDTELRYRQRYTDLIANPDVRETFRKRSKIISSIRKTLEDHGTLEVETPILSVLAGGA
                     NARPFKTFHNALGMEMYLRIATELYLKRLVVGMFGRVYEMGKNFRNEGLDTMHNPEFT
                     AMEVYWAYADYEDMMALTEEIIRKAAAVVRGPDSSGPVRYQDVDLDFDKPFKRGSMLD
                     LVREYTGVDFRTIADDDEARRIGKERGVEIKGTESRFTVLNLMFETFVEEKLIQPTFV
                     TGHPTEISPLAKRDPENPDYTRRFELFICGKEVANAFSELNDPIDQRARFEDQLKKKE
                     AGDDEAHDFDEDFINAIETGLPPTGGLGIGVDRLVMFLTDSRSIRDVILFPTMKPLVS
                     KSKDAPEA"
     gene            complement(2148..3260)
                     /locus_tag="PK442_00635"
     CDS             complement(2148..3260)
                     /locus_tag="PK442_00635"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOO86186.1"
                     /translation="MTLDELPVPPPSVAAHPVEIGGLRLANPLLLAPLAGVTIPALRL
                     LYSKLGAGAVHTEMISCAGLLVSERQSRKRNNTGDEGYEIVSPGSKTERMLAVLEGES
                     PVIAQLFAGDAETMARGAECALGVQSKIVALGINMACPMPKVLKKGAGSKLLERPEVA
                     FDMVRRLVALGLPVWPKIRKCPPTLPLTTEAFCEGLLEAGAATVTVHGRTPAQRYTGE
                     ADVSLLPRLATLFPGKVCASGDVFSPERAAEYLNGGCAAVMLARGAIADPFLFPRTLA
                     FLGYEVHNKYRNPSPDFQLSLLLDFGENVCAGSGPRIAVLMVKRLLSGMFKGLPGIGE
                     LRRAAAHILSWKELRGILETCEVYFERREEHSGCAS"
     gene            complement(3257..4024)
                     /locus_tag="PK442_00640"
     CDS             complement(3257..4024)
                     /locus_tag="PK442_00640"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOO86187.1"
                     /translation="MLLVLDVGNTTTVIGIYEGDTLKKHWRLMSERHTSDELGIYLLN
                     LLSISGISLKALEGAIYSSVVPSLDTALSEGLEEYLGVVPVKVTASLDLGMEIAYSPK
                     HEVGADRLVNSVAGKARFGAPLIVVDFGTAITLDILNKEGAYLGGTISPGLVTGMEAL
                     FGRTAKLPQVSLEPPKSVIGNNTIGSIQAGILFGNAGLVDSLVRRTWSELGTKTPVAA
                     TGGHASVVASLSETITHVDPWLTLEGLRLIYERNRAR"
     gene            complement(4153..5664)
                     /locus_tag="PK442_00645"
     CDS             complement(4153..5664)
                     /locus_tag="PK442_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOO86188.1"
                     /translation="MSREQYPLERLPRVERNHGPVPFVPREGAARAPLLFILAGLSVS
                     IMAVSRGIPAPVSSVLAPLTVWGLLLILSEYRPPRFFSYGAFLSLLAFACALSCGYRL
                     VSAATIKAGVLQDEGTVVLERSWGSRRIVLVDASSGTYLMRLSPNRYILEGDALFFTG
                     RTKAIAPRPGSSFREDLYWEARGVSIEVVPESITRKTGGWSLARWRTALRKRILLTLP
                     YRTRGYLLAAVLGVRDPDLTEAHRRWGTSHLLAVSGFHVGLVAFAVWRFLSFPALRLV
                     CSRRLAAVLSSLFLWGYVLLAGGAPGALRAALMIQALLLGTVLGRKGTPLNSVSLAAL
                     LLLLWRPAWFFDIGWRLSVMAALLLAGLGERRLSLWMLPLSSLFIWFAAFPQVTATFG
                     EVPVAGIALNLVALPLFSLLYPIALFLSLPLLLSVPSGEIFAGTAEGLFSLWEWVADS
                     AASFFPWTLSWSPLLATLGGGLFLLTVTGGLFPLRGRTIAGAGILLLAAVFFL"
     gene            complement(5661..6401)
                     /locus_tag="PK442_00650"
     CDS             complement(5661..6401)
                     /locus_tag="PK442_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO86189.1"
                     /translation="MENNGRLKRDRLASFAFFALGIGCFLLAGFLVYLFSGKFVNTPS
                     GMKAGPALSLSQVPAVTESAPAVLKEPPAAQKAWVLYVTGEVLSPGVYHLPPESRVFQ
                     LIEAAGGLTQRADPVQVNLAAPLDDGMHIHVPALLPPAPVLERKPGDYAAPSPGKSSS
                     AVVFPRASQPAVLRSAPQPAAGGRVNVNMATAAELESLPGVGPAIARSIMECRETLGR
                     FSCVEDLLKVKGIGAKKLEAMRGFIAIQ"
     gene            complement(6421..7755)
                     /gene="miaB"
                     /locus_tag="PK442_00655"
     CDS             complement(6421..7755)
                     /gene="miaB"
                     /locus_tag="PK442_00655"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOO86190.1"
                     /translation="MYGCQMNVYDGDKIRTALVDRGWRESPEDEADLVILNGCSIRDK
                     AEQKVWSDLGRFAVRWKNERKPIVAVTGCVAQSVGRRMAARFPWVRFVSGPRHIGAVP
                     DALEQFFTERDAGRTIGNEETVFLLDDDPRAFLDLPGAPTERVNRWKAFVTIAHGCDN
                     FCSYCIVPYVRGRFVSRPAEDILEEMRTLVADGVLEITLLGQNVNSYGSDFSDGYRFS
                     SLLRDAAASTGVRLLRFVTSHPRDFTPDIVEAMAEHTDILCPSINLPIQSGSDRILKM
                     MNRGYTLEQYRAAVSLIRNRLPGAGLTTDLIVGHPGETDDDFQCSVNALREFRFDLVH
                     SAAYSPREGTSAAAREDQIPREVKMARLNEINRIQSLIAREINESLVGSRFSVLLDDV
                     APRGEGILQGRTPSDKVVLVEGGEELLGRFVSVEITCGENWCLNGRVLHVEN"
BASE COUNT         1762 a         2349 c         2155 g         1491 t
ORIGIN      
        1 cagctcggcg aagtgctgcc gctgaagcaa caccgcgccg cgtacctcca tcttctgagg
       61 gaatggaccc gagaggcgca acgggagatt gcgtatagtc cgtatattcg cggtgacatt
      121 ctcgcccgtc ctcccgtccc cgcgtgtcgt tccctgcgcg aagacgccat cctcatacac
      181 aagagaaacg gcaaggccgt cgatcttcag ctcgcaaaga tagccatgtt caccggcgaa
      241 gggtgctgta cgggtgagaa acgccgagag ttcctccgta tcgagcgcat tatcgagact
      301 gagcatagga acggagagcc gtaccttggc aaacccctcg gccgcctttc ctccaacccg
      361 ctgcgtcggg gaatccggcg accggagaga agggtgctcc tcctccagcc gccgcagttc
      421 ggcgaagagc ccgtcgtact gatcgtccgg gatctcagga gtgtccagta catagtacag
      481 atgctcatgc cgcaggattt cctctcgtaa aaaaacaacc cgcgaacgta cttcttcagg
      541 atcccggcgt atcgtgctct cggccacctg gacgacgcct ctctttctac gcctccggcg
      601 cgtccttgct tttggagacg agcggcttca tcgtcgggaa gagaataacg tcgcgaatgg
      661 agcgcgaatc cgtaaggaac atcaccaggc ggtcgacgcc gatacccagt cccccggtcg
      721 gaggaagtcc cgtctcgatc gcattgatga agtcctcgtc gaagtcgtgt gcttcgtcgt
      781 cgccggcctc cttcttcttc agctggtcct cgaagcgcgc tctctggtcg atgggatcgt
      841 tcaactcgct gaatgcattg gcgacttcct tgccgcagat gaacagctcg aaacgccggg
      901 tatagtcagg gttctccgga tcgcgcttcg cgagcggcga gatctccgtc gggtgtcccg
      961 tcacgaatgt cggctgaatg agcttctctt cgacgaacgt ctcgaacatc aggttgagca
     1021 ccgtaaagcg gctttcggtt cccttgatct ccacgccgcg ctctttccca atcctccggg
     1081 cctcgtcgtc gtcggcgatg gtccggaagt cgactccggt gtactccctc acgagatcaa
     1141 gcatcgagcc tcttttgaac ggtttgtcga aatcgaggtc cacatcctgg tagcgcacgg
     1201 gaccggagga atctgggccg cgaacgacag ccgcggcctt cctgatgatc tcctccgtga
     1261 gcgccatcat atcctcgtag tccgcatagg cccagtagac ctccatcgcg gtgaactcgg
     1321 ggttgtgcat cgtgtcgagc ccctcgttcc ggaagttctt ccccatctcg tagaccctgc
     1381 cgaacatccc gacgacaaga cgcttcaggt agagctccgt tgcgatgcgc aggtacatct
     1441 ccatcccaag tgcgttgtgg aacgtcttga acgggcgtgc gtttgcgcca cctgcaagga
     1501 ccgagagaat cggcgtctcc acttcgagcg tgccgtggtc ttcaagcgtc ttccggatcg
     1561 aggagatgat cttggagcgt ttgcggaacg tctcgcgcac atcggggttc gcgataagat
     1621 ccgtatacct ctgacggtag cgcagctccg tatccttcag gccgtgccac ttttcgggaa
     1681 gggggcggag ggccttgcag aggagcacgc actgccgcac atggatcgtc agctccccgc
     1741 gctgcgttct gaagggatac ccgacgatgc ccacccaatc tccggtgtcc acccactttt
     1801 tcgcaaaggt gtactgttca tctcccatcg cgttgaactg gaggtagagt tgcatcgtct
     1861 ccgtttcgtc ggcaaggttg gcgaacatcg cctttccgtg cttccgaagc gtcatgattc
     1921 ttcccgcagt gacgatggaa acagactcgt cgatctcatc cgcttcgagg tgatcgaagt
     1981 tccgccggac gtattccaac tggtgctttc gttcccactt ctccaggata tacgggttgt
     2041 acccctcttc ggagagcaag cgctccagct tgtccatcct ctggcggact atttcatcgc
     2101 ccggaccgga ctcatctcct cccggaacgg tgttcaggac ttcctgctca tgatgcgcat
     2161 ccactatgtt cctctctcct ttcaaaataa acctcacacg tttcaagtat tccccggagt
     2221 tccttccagg agaggatgtg cgcagcggcg cgccgcagtt cgccaattcc gggaagccct
     2281 ttgaacatgc ccgacagaag ccgcttcacc atgagaacgg cgatcctggg ccccgatccg
     2341 gcgcagacgt tttcgccgaa atccagaaga agggaaagtt ggaaatccgg ggagggattt
     2401 ctgtatttat tatgcacttc atagccgaga aacgccagcg ttcgcgggaa aagaaacgga
     2461 tccgcaatcg ctccccgtgc aagcatcact gcggcgcacc ctccgttgag gtattccgcg
     2521 gcccgttcgg gggagaaaac atctccgctg gcacagactt ttcccggaaa aagcgttgca
     2581 agacgcggaa gcaaagaaac gtcggcttct ccggtatagc gttgcgcggg cgttcttccg
     2641 tgcacggtca ccgtcgcggc gcccgcttcc agaagccctt cgcagaacgc ctccgtcgtc
     2701 agcggcaacg tcggagggca cttgcgtatc ttcggccaga caggaagccc gagggcaacc
     2761 agccgcctca ccatgtcgaa ggccacctcg ggacgctcca gcaatttcga accggcgccc
     2821 ttcttcagta ctttgggcat cggacaggcc atatttatgc ccagagcgac gattttactc
     2881 tgaaccccga gtgcacactc agcccccctg gccatggttt ctgcatctcc tgcaaaaagc
     2941 tgggctatta cgggcgattc tccctccagg acggcaagca ttcgctcagt cttcgatccg
     3001 ggggagacga tctcataccc ctcgtctccg gtgttgttcc gcttccgaga ctgtctttcc
     3061 gatacgagca gtccggcaca gcttatcatc tccgtatgca ccgccccggc gccgagcttt
     3121 gagtagagaa gccgaagggc gggtatggtc actccggcca agggagcgag aagcagcggg
     3181 ttcgcaagcc gaagcccccc tatctcaacc ggatgtgcag cgacagaggg cggaggaaca
     3241 ggcaattcgt caagggtcac ctggcgcgat tcctttcgta gatcaggcgc agcccctcaa
     3301 gcgtcagcca cggatcgaca tgtgtgatcg tctccgagag cgacgcgacg accgaagcat
     3361 gtccgccggt ggcagccacg ggcgtcttcg ttcccaattc gctccatgtc ctccgtacga
     3421 gagagtcgac cagtccggcg ttgccgaaca ggataccggc ctgtatggag cctatggtgt
     3481 tatttccgat gacgctcttc ggaggttcaa gggagacctg cggcagtttc gccgtcctgc
     3541 cgaagagcgc ttccatccct gtaacaagcc caggagaaat ggtgccgcca agatatgcgc
     3601 cttctttatt gagaatatca agcgtgatag ccgttccgaa atcgacgaca atgagcgggg
     3661 cgccgaaacg ggccttccct gcgacggaat tcaccagccg gtcggctccc acctcgtgct
     3721 tcggagaata ggcgatctcc atcccaaggt cgaggcttgc ggtgactttt acgggaacga
     3781 cgccaagata ctcctccaac ccctcggaga gcgcggtatc gagagagggg accacgctcg
     3841 aatagatggc gccttcgagc gccttcagcg aaattccgga gatggaaagc aggttgagaa
     3901 gataaatacc caactcatcc gaagtatgac gttcggacat aagacgccag tgttttttca
     3961 gagtatcgcc ttcatagatt cctatgaccg tcgtggtgtt ccccacgtcg agaacaagca
     4021 acatgatatc ttcctccttg aacggaaggc tttaaacatc agagtattat accaccggaa
     4081 atatcttcaa ggaacactca gaaggcgggc tctcggaagg ctggaaaaca gaggcaagcg
     4141 gcaacgcgcc ctttaaagaa aaaacacagc cgcaagcagg aggataccgg cgcccgctat
     4201 tgtccgccct ctcaggggaa agagtcctcc tgtcactgtc aagagaaaaa gaccgccccc
     4261 aagcgttgca agcagcggag accatgagag cgtccaggga aagaaagatg ccgcgctgtc
     4321 tgcaacccac tcccagagag aaaaaagtcc ctcggctgtc cccgcgaaaa tctcacctga
     4381 aggcactgaa agcaaaagag gaagcgaaag aaaaagtgca atcggataga gcagtgaaaa
     4441 gagagggagc gcgacaagat taagcgctat tccggcgacg ggaacctctc cgaatgtggc
     4501 cgtcacctga gggaacgcgg caaaccaaat gaagagactg gaaagcggaa gcatccacaa
     4561 tgagaggcgg cgttccccga ggcccgcaag aagaagcgcg gccatcacgg agagacgcca
     4621 gccgatatcg aaaaaccagg caggacgcca gagaagcaga agcagcgcag caagggagac
     4681 tgaattcaaa ggagtaccct ttcgtccgag aacagtgcca agaaggagcg cctgaatcat
     4741 cagtgcagcg cggagagcgc ccggggcccc tccggcgaga agaacgtacc cccagaggaa
     4801 gaggctagaa agaacggcgg ccaatcggcg cgagcagacg agccgtagag ccggaaaaga
     4861 aagaaaacgc cacaccgcga acgccacaag gccgacatga aaacctgaaa ccgccaggag
     4921 atgcgacgtc ccccagcgtc tatgcgcttc cgtcaggtca ggatcgcgca cgcccagaac
     4981 cgccgcaagc aagtaccccc gcgtccgata cggcagcgta agaagaatcc gttttctgag
     5041 cgccgttctc cagcgggcga gcgaccatcc gccggtcttc cgtgtaatcg attcggggac
     5101 gacctcgatc gagacgcccc tcgcctccca gtagagatct tccctgaaag acgaaccggg
     5161 acgcggtgcg atcgctttcg ttctgcctgt gaagaagagt gcgtcccctt caagaatata
     5221 ccggttcgga gagagcctca tcaggtacgt tccagaggaa gcgtccacaa gcacaatccg
     5281 cctgctcccc caggaccgtt ccaaaacaac tgttccttcg tcctgaagaa cgcctgcctt
     5341 tatggttgcg gcgctcacaa gacgatagcc gcaagacaac gcacaagcga acgcgagaag
     5401 cgacagaaaa gcaccatagg agaagaaacg gggcggacgg tattccgaga gaatgagaag
     5461 gagtccccac accgtcagcg gcgcaagaac tgaagagaca ggagcgggga tcccccgcga
     5521 aacagccata atcgatacag agagaccggc gagaatgaag agcagcggcg ccctggccgc
     5581 tccctcacga gggacaaacg gaacggggcc gtgattcctt tcgacgcggg gaagccgttc
     5641 gagcggatat tgttcccggc tcattggatg gcgataaaac ccctcatcgc ttcaagcttc
     5701 ttcgccccga tccccttgac cttcagcaga tcttccacgc acgaaaatcg ccccagagtc
     5761 tccctgcatt ccataataga acgggcgata gccggcccta ccccgggaag gctttcaagc
     5821 tcggctgcag tggccatatt cacgttcacc cgccctcctg cggcaggttg cggggcgctt
     5881 cgaagcaccg ccggttggga cgcgcgcggg aacacaacgg cggatgacga ctttcccggc
     5941 gagggagcgg cataatcgcc cggttttctc tccaagaccg gcgccggagg aagaagtgcg
     6001 ggaacgtgaa tatgcatgcc gtcatcgaga ggagcagcca agttcacctg cacaggatcg
     6061 gctctctgcg tcaatccccc ggcagcttcg ataagttgaa acaccctgct ctcaggggga
     6121 agatggtaga ctccgggcga cagcacttcg ccggtaacat agaggaccca cgccttttga
     6181 gcggcggggg gttccttgag aacggcaggc gccgattctg ttaccgctgg aacctgagaa
     6241 agcgagagcg caggaccggc tttcatcccc gaaggagtat tcacgaattt gccggagaag
     6301 agatacacca ggaatcccgc gagaagaaaa cagccgatac caagggcaaa aaaagcgaaa
     6361 ctcgcaaggc gatcccgctt cagtctcccg ttattctcca tggtttcact gaatcctcct
     6421 ttagttttcg acgtgcagta cgcgcccgtt caagcaccag ttctctccgc atgttatctc
     6481 cactgagaca aaacgcccga ggagttcctc cccgccctcc acaaggacga ccttgtccga
     6541 cggcgttctg ccctgaagga ttccttcccc cctcggagcg acgtcgtcaa gcagcacgct
     6601 gaagcggctg ccgacaagcg attcgttgat ctcccgcgct atgagcgact gaatcctgtt
     6661 gatttcgttg agacgcgcca tcttgacttc acggggtatc tggtcctctc tggcggctgc
     6721 cgaggtaccc tcccttgggg aataggcggc cgagtggacc aggtcaaaac ggaattccct
     6781 cagagcattc accgagcatt ggaagtcatc atccgtttcc cccggatgac cgacgatgag
     6841 atcggtagtc aaccccgcgc cgggaaggcg gtttcgtatt aacgatactg ccgcacggta
     6901 ctgctccagg gtgtaccccc ggttcatcat ttttaaaatc cggtcgctcc ccgactgaat
     6961 agggagatta atcgaaggac agagaatatc cgtatgttcc gccatggctt cgacgatatc
     7021 cggggtgaaa tcccgcggat gcgacgtgac gaagcggaga agacgcacgc ctgtggaggc
     7081 tgctgcatct cgaagcaggg atgagaatct gtaaccgtcg gagaaatcgc tcccgtagct
     7141 gttgacgttt tgtccaagga gggtgatttc aaggacgccg tcggctacaa gcgtccgcat
     7201 ctcctccaga atatcctcag caggacggga gacaaatcgc ccccggacat acgggacaat
     7261 acagtatgaa caaaaattgt cgcagccgtg agcgatggtg acgaatgctt tccaccggtt
     7321 cacacgttcc gtaggagcgc ccggaagatc aagaaaagct cttggatcgt cgtccagaag
     7381 aaaaaccgtc tcctcgtttc cgatggtcct tcccgcgtcc ctttcggtga aaaactgttc
     7441 taaagcatcg ggaaccgcgc cgatgtgccg gggacccgac acgaaacgaa cccacgggaa
     7501 acgggcggcc atcctgcgcc caacgctctg cgcaacacag ccggtcaccg cgacgatcgg
     7561 ctttcgttca ttcttccaac ggacggcaaa acgtccaaga tcgctccaca ctttctgttc
     7621 cgccttgtcc cgaatgctgc acccgttaag gatcacgaga tccgcttcgt cctcggggct
     7681 ttctctccaa cctcgatcaa caagagcggt tcgtatcttg tcgccgtcgt aaacgttcat
     7741 ctggcatcca tatatct
//
LOCUS       DAOSWW010000016         7571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_6271_length_7571_cov_4.212214, whole genome shotgun sequence.
ACCESSION   DAOSWW010000016 DAOSWW010000000
VERSION     DAOSWW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6271_length_7571_cov_4.212214"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PK442_00660"
     CDS             <1..1293
                     /locus_tag="PK442_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOO86191.1"
                     /translation="RQDLGLPGAKEGCGEGECGACAVLLDGRLVNSCLIPAFQLPGRS
                     VETIEALSVDGTPDTLQQAFLDAGAVQCGFCTPGLVMASRALLSKNAHPTRKEIREGI
                     AGNLCRCTGYEAVVHAVGQAAEKGYAPSLVPAKRLDASVLLEKEEEGKVFLPRTLEEA
                     FEALSVHGDELAVVAGGTDLMVDMEKKGLPAPEKVLDITGIEGMRRIGVSGDWLEIGA
                     GATFAEIAVHENVLSVAPALAHCASQVGAVAIQNRATIGGNIATASAAADSPPVLMAL
                     GAVLVLASGKGSREMPVEEFFSEYRRTARRADELLVTVRIPLSVRGTRQMFRKVGLRQ
                     ALAISRVSVACVAALESGVVTSCRIAAGSMSPTPLLLKDVCSAVTGRALSRELAEEAG
                     RVADSLVSPRTAPEYRKETTGNLVRRFFTDILKENATV"
     gene            1518..1721
                     /locus_tag="PK442_00665"
     CDS             1518..1721
                     /locus_tag="PK442_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOO86192.1"
                     /translation="MTTQGTVKWFNATKGYGFVTCDDGKDVFVHYSAIQGDGFKTLDE
                     GQRISFRITQGSKGDQAADVEKI"
     gene            1949..3292
                     /gene="tig"
                     /locus_tag="PK442_00670"
     CDS             1949..3292
                     /gene="tig"
                     /locus_tag="PK442_00670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOO86193.1"
                     /translation="MRSEILSQENNVVSIKLEFEASEFAQGVEKAIKDLSTKVNIAGF
                     RKGHVPRKVMEMRFGRQAIYAEAIEAMLPDAIEQIVKDYDLDLIDEPHVSIEKMEEGS
                     PVDVRLTFEVSPEVVLPEVSAVTVHRPVASVADEIVSGTIEDIRAQNAEHSPVENVPV
                     AENHVVDIEYHTVVLLDEGKQERHGPDSTTLDLGQPSVRTEIRDALLGAKKGEFRTAD
                     VQVEENYQDPKVAGRTVRYELTVNEIKEKVLPEMTPDFFKKILRSDCETEELFREEIK
                     KRILEKIASEIQGKAEFDALQGISDLSQLTVPETLISRQMTAMKKEDEENIKKSRNMS
                     LEEMLSEASTTMEEYEKNIREQAETIVRRSLVLDKVADEMGITVEKEDFEAEMQTLAS
                     SYKIDAQRLVEGLFKDEKRLMEIANRIKYKKTMKAIMNTVQIDETEPAAEEKPAE"
     gene            3325..3903
                     /gene="clpP"
                     /locus_tag="PK442_00675"
     CDS             3325..3903
                     /gene="clpP"
                     /locus_tag="PK442_00675"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOO86194.1"
                     /translation="MLVPYVVEQTGRGERTYDIYSRLLKDRIIFLGDSVDEHTGNIVV
                     AQLLFLESEDPEKDIHLYINSPGGYVSSGLAIYDTMQYIKCPVSTICLGHAASMAAVL
                     LAGGATGKRFVLPNARIMIHQPLGGVQGQATEVEIHAREILKLRERLNDILVKHTGQS
                     KKKVRADTERDFYMSPEEAVEYGIVDKVIHSR"
     gene            4128..5438
                     /gene="clpX"
                     /locus_tag="PK442_00680"
     CDS             4128..5438
                     /gene="clpX"
                     /locus_tag="PK442_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOO86195.1"
                     /translation="MFRFDDRGRGSKSNGGKNLHNGRCSFCGKGQDEVQKLIAGPGVY
                     ICDECIHLCNMILVEEAGETAAKAKTAEPVFPLSEVPKPREIKEYLDQYVIGQDLAKK
                     AVSVAVYNHFKRISNNMAEESDVELQKSNVLLMGPTGCGKTLIAQSLAKKLNVPFAMA
                     DATTLTEAGYVGEDVENILVKLLQAADYDVKAAQRGIIYIDEMDKITRKSESASITRD
                     VSGEGVQQALLKILEGTLSNVPPKGGRKHPYQDFIQIDTSNILFICGGAFEGIEEIIG
                     RRVNKKIIGFGGDIMGKHDARRFNLMKQIQPEDLMAFGFIPEIAGRLPLLVPLEELNT
                     DALVRILVEPKNSIVRQYQKTFSMEGVSLEFTEDALRSIALKAGEKNTGARGLRAILE
                     NLMMDLMYDIPSRGKDVGKVIITKEAVEGSQPPLEEARSSEEVA"
     gene            5443..>7571
                     /gene="lon"
                     /locus_tag="PK442_00685"
     CDS             5443..>7571
                     /gene="lon"
                     /locus_tag="PK442_00685"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOO86196.1"
                     /translation="MKKEEEAQAQDIQDITEVSASGPRSCFVLPVRDIVIFPGIIAPL
                     FVGRPRSLKAIEMAMLQDRNVLVVAQKEMQVDDPRVEDLYSVGTVCSILQMVRIPDGT
                     TKVLIEGVERVQVVSYGKGKETLEADVVSLPWEEHHSPNLEALKRSVLEQFEKYVTLH
                     PRIPGEVLISVMNVEDPRQISDLVGSHLSLKVEKKQKLLEMVNLEKSLKFLLKVLLEE
                     IDILQLEHAIQDKVRQEVERGHKEYYLREQLKVIQDELGQGEKPSEIDELRTRIEEAE
                     MPEASLSKALHELDRLSKMPLMSAEATVVRTYIDWLVTLPWNTMTPDNLEIKRAQKVL
                     DEDHYGLEKVKERILEFLAVRKLALDKMKGQVLCFVGPPGVGKTSLGKSIAKALERKF
                     VNMSLGGMRDEAEIRGHRRTYVGAMPGRILQKIRQAGARNPVLLMDEIDKLGLDFRGD
                     PAAALLEVLDPEQNSSFTDHFMEVPFDLSDVIFITTANVTHSIPRPLLDRMEVIKIPG
                     YVWQEKIKIAKNHIFPRILEEHGLTPKQLKITPSGLEHVISEYTREAGVRGLERELST
                     ICRKVARRIVEDEKNAEEPVSIGPKHLKEYLGPPRLYDPRLPKTKEIGTVVRLAWTET
                     GGDVLVIEAVTMKGKGEVTLTGNLGSIMQESAQTAIGFLRSNADGFGIGNIDWKAIDV
                     HLHVPEGAIPKDGPSAGITMAVAILSAV"
BASE COUNT         1913 a         1842 c         2225 g         1591 t
ORIGIN      
        1 cgtcaggatc tgggcctgcc gggcgccaag gaggggtgcg gcgaggggga atgcggagca
       61 tgcgccgtgc ttctcgacgg ccgtcttgta aactcctgcc ttatccccgc tttccagctg
      121 ccgggcagaa gtgtggagac tatagaagcg ctctccgtgg atggtacgcc cgatacactg
      181 cagcaggcct ttcttgatgc cggtgcggtg cagtgcggtt tttgcactcc tggtttggtc
      241 atggcatcga gagcgcttct gtcgaagaac gcacacccta cacgtaaaga aattcgcgaa
      301 ggcatcgccg gaaatctctg ccgctgtacc gggtatgaag cggtggtgca cgctgtgggg
      361 caggctgcgg aaaaaggcta cgcacccagt cttgttccgg cgaagcgtct ggatgcctcc
      421 gtgcttctcg aaaaagaaga agagggaaaa gtgttcctcc ctcgaactct tgaagaggcc
      481 tttgaggcgc tgtccgttca cggagatgag ctcgccgtgg tcgcgggcgg caccgacctg
      541 atggtggata tggagaaaaa agggcttccc gcgcctgaaa aagttctcga tatcacggga
      601 attgagggga tgcgtcgcat aggcgtttcc ggtgattggc tggagatagg cgcgggcgcg
      661 accttcgctg aaatcgcagt gcatgagaat gtcctctccg tagctccggc tctggcgcac
      721 tgcgcttcac aggtgggcgc cgtcgcaatt cagaatcggg caaccatcgg aggcaacatc
      781 gctaccgctt cggcggcagc ggactctcca cccgtcttaa tggccctcgg cgccgtcctt
      841 gttttggcaa gcgggaaagg atcgcgtgaa atgccggtag aggaattttt ctcggaatac
      901 agacgcactg cccggcgtgc agacgaactg ctggtcacgg tgcgtatccc tctttccgtg
      961 agaggaaccc gtcaaatgtt ccggaaggtg ggtctccggc aggcgcttgc catttcacgg
     1021 gtttccgttg cctgcgtcgc agcgctggag agcggcgttg tgacttcgtg ccgaatagct
     1081 gcgggcagta tgtcgccgac gcccctgctc ctgaaggacg tctgctcggc agtcacaggc
     1141 agagctctga gcagagagct ggcggaagag gccggaagag tggccgattc gctggtgtct
     1201 ccccgtaccg cgccggaata caggaaggaa actaccggaa atttagtacg acgttttttc
     1261 accgatattc tcaaggagaa cgcgacggtc tgatcgatcc ggagcaggcc gcaaacggca
     1321 gagcgaaaag gagccggcag agcgccattt ttttgttttc tcgtgcggat tccggaagaa
     1381 acgcaggatg taacgaaaag aaagtgcgaa tttaaagcaa gaaagacttg acatgctctt
     1441 catttcttat tatgatgtat gcgttgacag gcgccgtcag caaaaagcac aagcaatcaa
     1501 gcttaggagg aacaactttg acaactcaag gcacagtaaa atggttcaac gcgacgaagg
     1561 gttatggatt cgtaacctgc gacgatggca aggacgtttt cgttcattat agcgctatcc
     1621 agggagatgg attcaagact ctcgacgagg gccagcggat ttccttccgt atcactcagg
     1681 gttccaaggg agatcaggct gccgacgtag agaagatata acttcgttcg acatatcgcc
     1741 atttccaagg cagcggtttt ccgctgcctt tttcttttat ttttggcgac tactttgctt
     1801 tgccagaaag agagcctgca tttctttgca ttctctcagt gttccgtgta caataccttc
     1861 cgcatatttt ctcttcctgt aggaatgaga tacattatag agaaaaaatt acaccagtgc
     1921 gaatcgcact gaaaggggtt gcgattgtat gagatccgag attctttccc aggagaataa
     1981 tgtcgtctcc atcaaacttg agttcgaagc atcggagttc gcccaaggag tagaaaaggc
     2041 gataaaagac ctgtcgacaa aggtgaacat agctggtttc cgtaaagggc acgtgccgcg
     2101 caaagttatg gagatgcgtt tcggcaggca ggcgatctat gccgaggcga tcgaagcgat
     2161 gcttcccgat gcaatagagc agatcgtgaa agattatgat ctcgatctca ttgacgaacc
     2221 tcatgtatcg attgaaaaga tggaagaggg gagtcctgtg gacgtccgcc tgaccttcga
     2281 agtttctccg gaggtagttc tcccggaggt ttcggctgtc accgtacatc gtcccgtcgc
     2341 ttctgttgcc gacgaaatcg tctccggcac aatagaggac atacgagcgc agaacgcgga
     2401 gcactctcct gtggagaacg ttccggttgc ggaaaatcac gttgtggata tcgagtatca
     2461 taccgttgtg ctgcttgatg aagggaaaca ggagcgccac gggccggatt caacaacgct
     2521 cgatctgggg cagccctctg tacgcaccga gatccgagat gcgctgctcg gtgcgaagaa
     2581 aggcgagttc agaactgcgg atgtccaggt ggaggagaat tatcaggatc ccaaagtcgc
     2641 aggacgtacc gtacggtatg aactgaccgt caatgagatt aaggagaaag tccttcccga
     2701 gatgacgcct gacttcttca agaagatact caggagcgac tgcgagacag aagaactttt
     2761 ccgcgaggag ataaagaagc gcatcctgga gaaaatagcc tcggagattc aggggaaagc
     2821 cgagtttgat gcgttgcagg gcatctccga tctttctcag cttacggttc cggagactct
     2881 gatttcccgc cagatgacgg cgatgaagaa agaggacgaa gagaacatta aaaagagccg
     2941 gaatatgtcg cttgaagaga tgctctccga ggcttccacg acgatggaag agtacgagaa
     3001 aaatatccgc gaacaggctg agaccatcgt ccgcagatcg ctggtgctcg acaaggtcgc
     3061 cgatgaaatg ggaatcaccg tggaaaaaga agactttgaa gcggagatgc agacgctcgc
     3121 ctcatcgtac aaaatcgatg cgcaacgcct tgttgaaggg ctcttcaaag atgaaaaaag
     3181 gctgatggag atagcgaaca ggataaaata caaaaagacg atgaaggcta tcatgaatac
     3241 tgtacagatc gacgaaacgg agcctgcggc ggaagagaag cccgctgaat aggaacaacg
     3301 ctacgatcac aggaggtgtt ccctttgttg gttccatatg ttgtagaaca gaccgggcga
     3361 ggcgaacgga catatgatat atacagccgt cttctcaagg acagaattat cttccttggg
     3421 gattcggtcg atgaacatac aggcaatatc gttgttgcac agctgctttt tcttgagagt
     3481 gaagatcccg agaaggacat tcatctctat atcaacagcc cgggagggta tgtctcctcg
     3541 ggactcgcta tttacgatac gatgcagtat attaaatgcc ctgtttccac gatctgcctt
     3601 ggacatgccg cgagtatggc cgcggttctt cttgcaggtg gagcgacggg aaaacgcttt
     3661 gttttgccga acgcacgcat catgatccac cagcctctcg gaggcgtgca ggggcaggcg
     3721 acggaagtgg aaattcacgc acgagaaata ctgaagctgc gtgaaaggct caatgatata
     3781 cttgtgaagc acacgggaca gtcgaagaag aaagtacggg ccgatacgga aagagatttc
     3841 tacatgtctc cggaagaagc tgttgagtac gggatcgtcg ataaagtcat ccattcgaga
     3901 tagagcgcgc ttcagttccg gaaacaactg ttttgattcg acttcaaggg agcccgagac
     3961 aagcaggcaa cgctccggcg cgtttatgga aacgcaccgt cggcatagag ggaaaatacc
     4021 cttcttgccc gggagtttgt cggcttgcat tcctgtacag gggatgggag ttcaggcgct
     4081 cccttgttgc tgtatcccgg gtagagaggt tgcagggagg tttatgaatg ttcaggttcg
     4141 atgacagggg aagaggttcc aagagtaacg gcggcaaaaa tctccataac ggacgctgtt
     4201 cgttctgcgg aaaagggcag gacgaggtgc agaagctcat agcaggcccc ggcgtgtata
     4261 tttgcgacga gtgtatccat ctgtgcaata tgatcctggt cgaggaggcc ggggagactg
     4321 ctgcgaaagc gaaaaccgca gagcccgtct ttccgctttc ggaggttcca aaaccacgag
     4381 agataaaaga gtatctggat cagtacgtca taggtcaaga tctggccaag aaagccgttt
     4441 cggtcgcagt atacaaccat ttcaaacgga tcagcaacaa catggcggaa gagagcgatg
     4501 tcgagctgca aaagagcaac gtcctgctga tggggcctac cgggtgcggc aagacgctga
     4561 ttgcgcagtc cttggcgaag aagctcaacg ttcccttcgc tatggccgat gcgacgacgc
     4621 tcacagaggc cggttatgtc ggcgaggatg tcgagaatat cctcgtaaaa ctgcttcagg
     4681 ccgcggacta cgatgtaaag gccgcgcaga gaggcatcat ctacattgac gagatggaca
     4741 aaatcaccag aaagtcggag tcagcctcaa taacaagaga cgtctccggc gaaggtgtgc
     4801 agcaagcgct cttgaagata ctcgaaggca cgctttcaaa tgttccccca aagggcggaa
     4861 gaaaacaccc gtaccaggat tttatccaga ttgacacgtc gaatattctc tttatttgcg
     4921 gcggagcatt cgaaggtatc gaggaaatca ttgggcgcag agtgaacaag aagatcatcg
     4981 gttttggcgg cgacatcatg gggaagcacg atgcccgacg ttttaatttg atgaagcaga
     5041 ttcagcccga ggaccttatg gcgttcggct tcataccgga gatcgcgggg cggcttccgc
     5101 tgctcgttcc ccttgaagaa ctgaatacgg atgcgctggt ccgtattctt gttgagccga
     5161 agaactctat tgttcgtcag tatcagaaga ccttctccat ggaaggggtg tccttggagt
     5221 tcaccgagga tgctcttcga agcatcgcct taaaagccgg ggagaaaaac acaggcgcca
     5281 gagggcttcg cgcaatcctc gagaacctga tgatggacct gatgtacgat atcccctcca
     5341 ggggaaagga cgtcggcaag gtgatcataa ccaaagaggc cgtcgaagga tcgcagcccc
     5401 ctctggaaga agcgagaagc tcggaggagg ttgcctgata tcatgaagaa agaagaagaa
     5461 gcgcaggcgc aggatataca ggatataacg gaggtttccg cttccggtcc gcgcagctgt
     5521 tttgtccttc cggtacggga tatcgttatc ttccccggta tcatcgcgcc gcttttcgtg
     5581 gggcgtcccc gttccctgaa ggcaatcgag atggccatgc tccaggacag gaatgtcctg
     5641 gtagtcgctc aaaaagagat gcaggttgac gaccctaggg tagaggatct ctattcagtg
     5701 ggcactgtat gcagcattct ccagatggtc aggattcctg acggaacgac gaaagtgctt
     5761 atcgagggcg tggagcgcgt tcaggttgtt tcgtacggta agggaaaaga gactctggag
     5821 gctgatgttg tatccctgcc gtgggaagag caccactccc cgaacctgga agctttgaag
     5881 aggagcgttc tggaacagtt tgagaaatac gtgacgctcc acccgcggat tccgggcgaa
     5941 gtgcttattt cagtgatgaa cgtagaggat ccccggcaga tttccgattt ggtcggctcg
     6001 cacctctcgc tgaaggtgga aaagaaacag aaactgctcg agatggtcaa tcttgaaaag
     6061 agtctgaagt ttctcctgaa ggtcctcctg gaagagatcg atatcctaca actggagcac
     6121 gctatccagg acaaggtgcg ccaggaagtt gagcgggggc ataaggagta ctacctcagg
     6181 gagcagctga aagtgatcca ggacgaactc ggacagggtg aaaaaccttc cgagatcgat
     6241 gagttgcgga cgcgcataga ggaagcggag atgccggaag cctctttgag caaagcgctt
     6301 cacgagttgg accggctttc aaagatgccg ctcatgtccg cagaggcaac cgttgtacgg
     6361 acgtacatcg actggcttgt gaccctcccg tggaatacga tgacgccgga caacctggag
     6421 ataaaaagag cgcagaaagt tcttgacgaa gaccactacg gcctggagaa agtgaaggag
     6481 cgtatcctgg aattcctggc tgtccgcaag ctcgcgctgg acaagatgaa ggggcaggta
     6541 ctctgctttg tcggccctcc gggagtggga aagacctcgc tcgggaaatc catcgcaaag
     6601 gcgctcgaac gcaaatttgt caatatgtcg cttggcggta tgcgggacga agccgagatt
     6661 cgcggccaca gaagaaccta tgtcggcgcg atgccgggcc gcatattgca gaagatacga
     6721 caggcaggcg cacggaaccc ggtgcttttg atggacgaga tcgacaaact cggacttgac
     6781 ttcaggggag atcctgcagc cgcgcttctg gaggtacttg atccggaaca gaacagctcc
     6841 tttaccgacc acttcatgga ggtgccgttc gatctgagcg acgtcatctt catcaccacg
     6901 gccaatgtta cccactctat ccccagacct ctgctcgaca gaatggaggt catcaaaatc
     6961 ccggggtacg tatggcagga gaagataaag atagcgaaaa accatatctt cccgagaatt
     7021 cttgaagagc acgggcttac tccgaagcag ttgaagataa cccccagcgg gctggaacat
     7081 gtcatcagcg agtacacccg ggaagcggga gtccgcggcc tggaacgaga gctctccacc
     7141 atctgccgaa aggtggccag gaggatcgtc gaggacgaga agaatgccga agagccggtt
     7201 tcaataggcc ccaaacacct gaaggagtac ctcggcccac cgagactgta cgatccccgt
     7261 cttccgaaga cgaaggagat cggcaccgtc gtgaggttgg catggacgga gaccggcggc
     7321 gatgtgctcg tcattgaggc cgtcacgatg aagggaaaag gagaagtcac ccttacaggg
     7381 aatcttggct ccatcatgca ggaatccgct cagaccgcga taggttttct ccgttcgaac
     7441 gcggatggtt ttggcatcgg aaatatcgac tggaaggcta tcgacgtcca tcttcatgtc
     7501 ccggaaggcg ctattcccaa agacggcccc tccgcaggaa tcaccatggc ggtagccatt
     7561 ctttccgcgg t
//
LOCUS       DAOSWW010000017         7455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_6425_length_7455_cov_4.509730, whole genome shotgun sequence.
ACCESSION   DAOSWW010000017 DAOSWW010000000
VERSION     DAOSWW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6425_length_7455_cov_4.509730"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK442_00690"
     CDS             <1..268
                     /locus_tag="PK442_00690"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOO86197.1"
                     /translation="VTGADEKGNAHLEEYMQRVRASTKLPLALGFGVSTPHRAKAAAQ
                     VADGVIVGSALISALEPCGTDRKQLVAKATEFIGSLRAGVDSAG"
     gene            432..665
                     /locus_tag="PK442_00695"
     CDS             432..665
                     /locus_tag="PK442_00695"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOO86198.1"
                     /translation="MPTPISVRLDEETGKRLDRLAKETGRTKAFYIRKMIEERIDDME
                     DYYLAAERLAQSRKFGSRSLTSEEARRELGLED"
     gene            649..915
                     /locus_tag="PK442_00700"
     CDS             649..915
                     /locus_tag="PK442_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO86199.1"
                     /translation="MAWRIEYDPSALGDLRHIDRRMAQRIVDYMDRRVAMMDNPRTLG
                     KALKGGFGDFWRYRVGDYRVICSIEDKNVMVLVVRVGHRSDVYR"
     gene            978..1784
                     /locus_tag="PK442_00705"
     CDS             978..1784
                     /locus_tag="PK442_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86200.1"
                     /translation="MRRCLYSTVFSLFLLFSAPAAAVDFTGIWTFSKEDGTEYSFDLV
                     QRDTALEGTFSTYDPLLDKYGEAHIDGSVEGRSASFAAGDEKGALAMEEGDVSAKWTL
                     KRAGSEGLLPPSGSVVRKDFGAMGFRLLAEEKLFVLDDAETEKDVLKHLGKPEKKSKA
                     ELWGADGLIHQQWFYKKKGIAVDFVKEDKSGPGEAYSVSISAPSAKKTSRGIGIGSAQ
                     QEVLKAYAAEINREESDLPETVVVGSIYGGVVFTMKNGKVVSIFIGAAAE"
     gene            complement(2053..2355)
                     /locus_tag="PK442_00710"
     CDS             complement(2053..2355)
                     /locus_tag="PK442_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86201.1"
                     /translation="MDEKTIYKQKIEARLALAHAQHKLNDLKTLAQNCPEDKVLEFSK
                     GIEELEKNFIHAKLKFREMNEAGDEAWRNFKESAEQIWNALRVSVKNATARYKELH"
     gene            2936..3241
                     /locus_tag="PK442_00715"
     CDS             2936..3241
                     /locus_tag="PK442_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86202.1"
                     /translation="MDDKSTQRMISDEDRTALRLLQHFCYTIGSANDAEDHGYGGEAR
                     RMREESCESIRNLADQHPLLTEFFPGLKEELETGRFLAFGWSSIAREADELLAGGAL"
     gene            3238..3558
                     /locus_tag="PK442_00720"
     CDS             3238..3558
                     /locus_tag="PK442_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86203.1"
                     /translation="MNIPGEDRTQCDVCSSLSESEYGYSKYGWPDHDVDLPDAAGSLV
                     MVKDLKPLSERKLQLWRCPGCGAWFLYTTDYEYLTNGTEDEQFLTRLSEEEAAEYLNR
                     PEAP"
     gene            complement(3678..3974)
                     /locus_tag="PK442_00725"
     CDS             complement(3678..3974)
                     /locus_tag="PK442_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86204.1"
                     /translation="MYALTKEAYRRKIQARLGLAHAQAKLNELKEKNKTSSANPVGEY
                     SNEINELEKNIEETQKRLNELYEAGDEAWEHLDDTSERILNALRTAVKNAVAKQ"
     gene            complement(4103..4324)
                     /locus_tag="PK442_00730"
     CDS             complement(4103..4324)
                     /locus_tag="PK442_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86205.1"
                     /translation="MDIVKIIAIGLIATGALGLTYGGFTYTQTTHVAGLGPIRFSVDG
                     NRTVYIPLWASGTAVALGGILLLFGGKLI"
     gene            4850..5656
                     /locus_tag="PK442_00735"
     CDS             4850..5656
                     /locus_tag="PK442_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOO86206.1"
                     /translation="MDQQGENAADKQGIRVLERLFDILSFLAERQDYAGIKEIAEATG
                     LSRTTVHRVLATCEENYVVLKDNIGRYRIGPRSLVWANSYQQQTGLAKIARQHLKELL
                     RDVGETVHLFIYEKGEAFYLEKMHTYNVTGLDSRIGSRLELYSTSAGRAILAALPEEE
                     FEAYLSSRELLPRTDWTVVDKETFGKIIEKARTEGYAEETQENEVGVRCVGAAILGKN
                     GYPLGAVSVAGPVFRFTDDRVESVGKRVFGTARIISYQLGYSTNGGQGGL"
     gene            5677..6510
                     /gene="kduI"
                     /locus_tag="PK442_00740"
     CDS             5677..6510
                     /gene="kduI"
                     /locus_tag="PK442_00740"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOO86207.1"
                     /translation="MKLDIRHGANPCDFKSYDTERLRKDFLVTHLFVPGEIRMTYSHI
                     DRIITGGACPTIPLSLECGKEIGTEFFLQRREMGIINVGAPGSVTVDGERVSLKKMDG
                     LYLGAGGKNIVFESDKHDDHAHFYFSSTPAHTSYPNCYIDMEKAKKVKMGEPEKANVR
                     TINQYIHPEVCTTCQLMMGLTQLENGSVWNTMPCHTHERRMEVYFYFDIPQDGVTFHI
                     FGQPEETRHIVVRNEEAVINPSWSIHSAAGTSNYSFIWSMAGENQVFTDMDHVPMSAM
                     K"
     gene            6674..6901
                     /locus_tag="PK442_00745"
     CDS             6674..6901
                     /locus_tag="PK442_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86208.1"
                     /translation="MDWGRVPADTVVIESKEITLRDVVQAAADGVDTPEGLMEVLGLE
                     EGQEGTEHLQPILDVFLPAIERLRSGSCGGG"
     gene            complement(7012..>7455)
                     /locus_tag="PK442_00750"
     CDS             complement(7012..>7455)
                     /locus_tag="PK442_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HOO86209.1"
                     /translation="EDGQMFTLSVVPADDFADIAEDAIKAHKGWVANLKKFVEGGQWD
                     LETNPQRCRFGIFLSFIERPEGASEELWSGILSMHEKLHGLGHTVNDAMQRGESGKAR
                     EVLKETVALSERLSASLLRVVEICRGQGEQEREASGLPALPERTR"
BASE COUNT         1670 a         1958 c         2153 g         1674 t
ORIGIN      
        1 ggtgacgggc gccgatgaga aaggaaacgc gcacctcgaa gagtacatgc agcgagtccg
       61 cgcttccacg aagctcccgc tcgccctcgg cttcggcgta agcacgcccc atcgggcgaa
      121 ggccgcggcg caggtcgccg acggcgtgat cgtcggcagc gcgctcatct ccgcgctgga
      181 gccgtgtgga accgacagaa aacagctcgt ggccaaggcg acggagttca tcggctccct
      241 ccgcgcaggc gtcgactcgg cggggtaggg ggaagtccgg cgctttcggc aacggacaac
      301 ggaatacaga gtgaaacgcg cgcttgcgat tgtgccgatc aggcatgtcg cggcgcgttt
      361 tttggttgac agcatcgatc ggcgcgctac aatgactaca aagactacgt tttaaaaaag
      421 gaggcgttgc tatgcctacg ccaatatccg tgcgactgga cgaggaaacc ggaaaacggc
      481 tggaccggct ggcgaaagag accgggcgga cgaaagcgtt ttacatacgg aagatgatag
      541 aagagcggat cgacgacatg gaagactatt atcttgccgc agaacgactt gcgcaaagcc
      601 gaaaattcgg ctcccgcagc ctgacatctg aagaagcgag gcgtgaactt ggcttggagg
      661 attgaatacg atccttcggc acttggagac cttcggcata tcgaccgtcg gatggcgcag
      721 cgtatcgtcg attatatgga cagaagagtc gccatgatgg acaatcccag gactttgggg
      781 aaagcgctca agggaggatt tggcgatttc tggcgctatc gtgtaggaga ttaccgtgta
      841 atttgttcga ttgaggacaa aaacgtgatg gttttggtag tccgtgtagg tcaccgcagt
      901 gatgtgtaca gataaggtga agcatatatg gatgtatttc gggacgatgc aacgagtttt
      961 tttgggaggg gtatgctatg aggcgttgct tgtattctac cgttttctct ctgttcctgc
     1021 tcttctccgc gccggcggcg gcggttgatt ttacggggat atggaccttt tcaaaggagg
     1081 acgggacgga gtactcgttc gacctggtcc agcgcgatac ggcgcttgaa ggcacgtttt
     1141 cgacctacga tccgttgctg gacaagtacg gggaggcgca tatcgacggc tccgtggagg
     1201 ggcgcagcgc atcgttcgcg gccggggacg aaaagggcgc gctcgcgatg gaggaggggg
     1261 acgtctccgc gaaatggacg ctcaaacgcg ccggaagcga ggggctcttg cccccgtcgg
     1321 gcagcgtggt gcgcaaggat ttcggcgcga tggggttcag gctgctcgcc gaggaaaaac
     1381 tcttcgtcct cgacgatgcg gagacggaaa aggacgttct caagcacctc ggcaagccgg
     1441 agaagaagtc gaaggccgag ctgtggggcg cggacgggct catccaccag cagtggttct
     1501 ataagaagaa gggaatcgcc gtcgatttcg tcaaggagga caagtccggc cccggcgagg
     1561 cgtattcagt ttcgatctcc gcgccgtcgg caaagaagac gagccgggga ataggaatag
     1621 ggagcgcgca gcaagaggtg ctgaaggcct atgcggcgga gatcaaccgc gaagagagcg
     1681 atctccccga aacggtcgtc gtcggttcca tctacggagg cgtcgtcttc acgatgaaga
     1741 acggcaaagt ggtctccatc ttcatcggcg cagcggcgga gtagcaatca gctgatagaa
     1801 atgttcaaat tcaatgcagc ctaaggcgtc cggaacggtt acgacttagg ctgcatcagg
     1861 ttctgcacgt ttcatttatg attgaggatc atcgatgcgt ctacggaccc gttccctcca
     1921 agaagcgcgg gaacatagga gcaggggcgc atcgttgatg ccgttcttaa aaacgaacgg
     1981 gaagcgcccc aaggagagtc gttaaaaaac aacgaccgac actctcttgc atgcgtcggt
     2041 cgttgttctc ttttaatgta actccttgta tctggcagtt gcatttttga cggatactct
     2101 caatgcgttc cagatttgct ccgcgctctc cttgaaattt ctccacgcct catcgccggc
     2161 ttcattcatc tccctaaatt tgagcttcgc gtggatgaaa ttcttttcaa gctcttcgat
     2221 ccccttgctg aattctagga ccttgtcttc cggacaattt tgagccaggg tcttcaaatc
     2281 gttcaatttg tgttgcgcgt gcgccaacgc caagcgcgct tctatcttct gtttgtagat
     2341 tgttttttcg tccacaggcg tcacctcctt atagtagata cagtatttgt tgttatgaaa
     2401 atttttcgtg cgcgctgcga gcatttccat gaggcgactt ccaatgaaaa tacgcgccat
     2461 ttgccaaata tattgaaatt cttaatgaat agaaacatat acatcgaatt caattatacg
     2521 gtaaatttta aaaatcacaa gagggggttt gtattttcac agtataagga atctctaaat
     2581 aaaggagttt tcgccttata cggctcgata gaatctcttt tcacaagacg tggaacagtt
     2641 tttttgatat cccttaagag ttctttctat tattgccggc ttgatgttga tcgaagcacg
     2701 aagcgccccg agttttcaat agcagttccg tatggagcgc ttctgcgttt ttgtctccga
     2761 aggtatgcta tgcatctttt ccggccgggg agtataatcc gtaagaggac agtgtgattt
     2821 caatggcttt taagctgttc ggaacgcgat tttcagggga gggataggtt gtgacacgtt
     2881 ctttctgttc ggccgttttt ccgcttcatc cgttcttcac tgcgggggtg gtgtgatgga
     2941 tgacaagagc acgcagcgaa tgatcagcga cgaggaccgt accgcgcttc gcctgctgca
     3001 gcacttctgt tatacgatcg gcagcgcgaa cgacgcggag gatcacggat acggcggaga
     3061 ggcgaggcga atgcgggagg aatcctgcga gtcgatccgg aacctggcag accagcaccc
     3121 tctcctcacg gagttctttc ccggactgaa agaggaactg gaaaccgggc gttttctcgc
     3181 gttcggatgg agctcgatcg cacgcgaagc ggacgagctg ctcgccggtg gcgccctatg
     3241 aatatccccg gggaagaccg cacgcaatgc gacgtctgtt cgagtctttc ggagagcgag
     3301 tacgggtaca gcaaatacgg ctggccggac cacgacgtcg atctgccgga tgccgcaggt
     3361 tctctcgtta tggtgaagga tctcaagccc ttgagcgaga ggaagctgca actctggcgg
     3421 tgtccggggt gcggcgcatg gtttttgtac acaaccgatt acgagtactt gacgaacgga
     3481 acggaggacg agcagttcct gacccggttg agcgaggagg aagccgccga atacctgaac
     3541 cggccggaag ctccgtagtc ctgacggttg ctgttatcgc atcctcgttg cctttgcagg
     3601 ctgtcggtta cagacaacgg ttttttaaac ctcgttaaac gccctgaacg ggtgattatc
     3661 gaggcttcaa agtatcctta ttgttttgcg acggcatttt tcacggcggt ccgtaacgcg
     3721 ttcaatatcc gctccgatgt gtcgtcgaga tgctcccagg cctcgtctcc ggcttcatac
     3781 agctcgttca ggcgtttttg cgtttcttct atattctttt caagttcgtt gatttcgttg
     3841 ctgtattctc caaccggatt cgctgaagaa gtcttgtttt tctccttcag ttcattgagt
     3901 ttggcctgcg cgtgcgccag ccccaatcga gcctgtatct tacgtctgta ggcttccttt
     3961 gtaagtgcat acactacgcc atctcctctt gttgcctccc aagttctttc cggtttttcc
     4021 cggcgcgcat gaaaaacaac gcccgtgacc gggttccaat ctctttgctg aatgcaagag
     4081 cggcatctgc cggaaagaca gtttatatca gttttcctcc gaagagcaga agaattccgc
     4141 cgagagcgac cgcggtcccg ctcgcccaaa gcggaatata gacggtccga ttcccgtcca
     4201 cggagaatcg gatcggcccc agtccagcca catgggttgt ctgcgtatag gtgaatcctc
     4261 cataggtcag ccccagtgcg ccggttgcga tcagccctat tgcaatgatt ttcactatgt
     4321 ccattcgact ccctcctccg ggaaaaatgc tttgatttcg ctgtgaacgc cgaaagaatc
     4381 gcattgtcgt ccgtgtgaaa atcgggaagc cggcgagcga cgttgttgcg ggacaacccg
     4441 acgtgatttc attgcctgtc ggcgagttga tcgcttcgtc cttccgctca caacgaaatt
     4501 attatatatc attttgaaaa aacagtcaag tgaaaattta atattatatt tgcattttac
     4561 gcctatttat tattggttta ttcagtataa agtgattttt tcaggctgtt ttcagggttg
     4621 tatgctgtat tatgagacgc ctcgctctca gcgcgttttt tggtcgtcga acgagcgtat
     4681 cggattttgg tattgtaaaa atcaaaaaac aggggtataa tgcccaataa gcggatttta
     4741 aaatccgcct gtcggaataa agatgctgct ttacggataa aaagaagaaa aatttcatca
     4801 ggtggaacct tgtcgaaggg aaagcagagg agtgtagagc gtgagaataa tggaccaaca
     4861 gggagaaaat gctgcggaca agcagggaat ccgcgttctt gaacggctct tcgatatcct
     4921 ctccttcctt gccgaacggc aggactacgc tgggatcaag gagatcgccg aagccaccgg
     4981 actgtcgcgg acaacggtgc atcgcgttct ggcgacctgc gaggagaact atgtcgtcct
     5041 taaggacaat atcggtcgct atcgcatcgg tccgagaagc cttgtctggg ccaactccta
     5101 ccagcagcag acggggctgg ccaagatcgc gcgtcagcat ctgaaggagc tgcttcgcga
     5161 cgttggcgag acggtccacc tcttcatcta tgaaaagggc gaggcgttct atctggagaa
     5221 gatgcatacc tacaacgtca cggggcttga ttcgcgcatc gggtcgcggc tcgagctgta
     5281 cagcacttcc gcggggaggg ccatactggc cgcgcttccg gaggaggagt tcgaggcgta
     5341 cctctcttcc agagaactgc ttccgcgcac cgactggacc gtcgtcgaca aggagacgtt
     5401 cgggaagatc atcgagaagg cgcgaacaga agggtatgcc gaggagaccc aggagaacga
     5461 ggtcggcgtc cgctgcgtcg gcgcggcaat cctcgggaaa aacggctacc ctttgggggc
     5521 ggtcagcgtc gccggccccg tcttccgttt tacagacgac agggtggaga gcgtgggcaa
     5581 gcgcgtgttc ggcactgcgc ggattatatc ctaccagctt ggttacagta caaacggagg
     5641 ccagggcggt ctctaaaaaa taaggaggga ctcatcatga aactcgacat ccggcacgga
     5701 gcgaatccgt gcgacttcaa gagctacgac accgaacggc tgcggaagga ttttctggtt
     5761 acccatcttt tcgtccccgg agagatcaga atgacgtaca gccatatcga ccgcatcatc
     5821 accggcggcg cctgcccgac catccccctg tcgctggagt gcggcaagga gatcgggacg
     5881 gagtttttcc tccagcggcg cgagatgggg atcatcaacg taggggctcc cggctcggtc
     5941 accgtcgacg gagagcgcgt ctccctcaag aagatggacg ggctctacct cggagcgggc
     6001 ggcaagaaca ttgtcttcga gagcgacaaa cacgacgatc atgcgcactt ctacttcagc
     6061 agcacccctg cccatacttc gtaccccaac tgctacatcg acatggaaaa agcgaaaaag
     6121 gtcaaaatgg gcgagccgga aaaggccaac gtccgcacta tcaaccagta catccacccg
     6181 gaggtatgca ccacctgcca gctcatgatg gggctcacgc agctcgaaaa cggcagcgtc
     6241 tggaacacca tgccctgcca cacccatgag cggcgcatgg aagtgtactt ctacttcgac
     6301 attccccagg acggcgtgac gttccatatc ttcggccagc ccgaggagac ccgccacatc
     6361 gtcgtccgta acgaggaggc cgtcatcaat ccgagctggt ccatccacag cgcggcgggc
     6421 acgtcaaact acagcttcat ctggagcatg gcgggagaaa accaggtgtt caccgacatg
     6481 gaccacgttc cgatgtcggc gatgaaatag gaaaatgcaa ggggctccat tcgaagaaaa
     6541 taccctctag cggcatatac gaaaaaacac ctatggcgca tttgtcgaaa gaaggtataa
     6601 tatcgaaagc tttttcttcc ggagcgccat gttcggaaaa agggctgact ttactgagga
     6661 aggaggagtc gtcatggatt ggggaagagt gcccgcggat accgtagtga tcgagagcaa
     6721 ggagatcacg ctgcgcgacg tggtgcaggc ggcagccgac ggcgtcgaca cgcccgaggg
     6781 actgatggaa gtcctcggcc ttgaagaggg gcaagaggga accgaacacc tgcagcccat
     6841 tctggacgtc tttcttccgg ccatcgaacg cctccggagc ggcagctgcg gcggcgggtg
     6901 agccggttgt tccggcagct gttagctgct gattgaagac gaaaaagagg gccgaggcat
     6961 ccccttgcgg ggggcttcgg ccctcttctt ttattgaatg atcctttccg cttaccgcgt
     7021 tctttccgga agcgccggga gccccgaggc ttcccgctcc tgttccccct gcccgcggca
     7081 gatctcgacc acgcggagca gggatgccga aagacgttcg ctgagggcta cggtctcctt
     7141 caggacctcc cgcgccttgc cgctctcgcc gcgctgcatc gcgtcgttga ctgtgtgccc
     7201 cagaccgtgc agcttctcgt gcatcgagag gatgccgctc cacagctctt cggaagctcc
     7261 ctcaggccgc tcgatgaagg agaggaagat gccgaagcgg cagcgctgcg gattcgtctc
     7321 caagtcccac tgtccgccct ccacgaactt cttcagattg gccacccagc ccttatgcgc
     7381 cttgattgcg tcttccgcga tgtcggcgaa gtcgtcggcc gggaccacag agagggtgaa
     7441 catctggccg tcctc
//
LOCUS       DAOSWW010000018         7366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_6522_length_7366_cov_4.027630, whole genome shotgun sequence.
ACCESSION   DAOSWW010000018 DAOSWW010000000
VERSION     DAOSWW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6522_length_7366_cov_4.027630"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PK442_00755"
     CDS             complement(<1..1323)
                     /locus_tag="PK442_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO86210.1"
                     /translation="MKLYGKILAPMLLLFFLGIATVSFTGYALSLRVLTEASQQKVLQ
                     AADIAEAMQRVGVVSSLIALGVVLVAGIVMLLVVRSIVKPLRDVVRVLVRRSELDFTV
                     NPADLWMIKRSKAKDEIAEMIQALILLRVKVEEMLRPLLTRSEAFSKSAQSLAELSEE
                     TVASMQEVKSSIETVFSLSEENAEAIRRTESGVGDVASSAEIAAASAQEGAKSASATS
                     ETSRLAVEKVEQVIGKIRTVETRTGESARSMGSVSASVESISRFVNTIKSIADQTNLL
                     ALNAAIEAARAGEAGRGFAVVADEVRKLAEESNHAAKEVEVLIGSLQNGTKEAMDVTN
                     ETGGVMKETVAAAEEAQRDLEDALERIGRMDGLMRNLADASGKQEAASREMTASIETA
                     SSGIASLTATFAGIRSAAEDTAGASERVAREAADLASGAGEVRSLVERF"
     gene            complement(1442..2239)
                     /locus_tag="PK442_00760"
     CDS             complement(1442..2239)
                     /locus_tag="PK442_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HOO86211.1"
                     /translation="MKLRVGVLQLDIAIGDKQRNCQNIEDWMASFYVPSELPTAVVVP
                     EMWNTGYALSRAKELADPEGAEAAAFLGELARKYGVWFVGGSVLASTKKGYANRAQAI
                     NPEGKLVAQYDKAHLIRLMEEEKYFTGGRKACLFDLEGAKAGCVICYDIRFCEWIRTY
                     ALKGAEVLFVSSQWPSARADHWRTLLRARAIENQMYVVACNRCGTSGDTHFGGGSMIL
                     GPKGEIYFEGGGGEEAGFATIDLDEVAETRNFLTVFNDRVPEIYSVK"
     gene            2549..2920
                     /locus_tag="PK442_00765"
     CDS             2549..2920
                     /locus_tag="PK442_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86212.1"
                     /translation="MAYITCEVCGTYIDHNYNTTKIIGRYNSMALLCEWHHAVVSALI
                     ARHGTVNWRALGRATSQVQEEKPNVLTEPERWLDYDSVARLKELYEEEIVRDPTDWPP
                     YESPNAKRAEEEEASDRYLYR"
     gene            2941..3228
                     /locus_tag="PK442_00770"
     CDS             2941..3228
                     /locus_tag="PK442_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86213.1"
                     /translation="MPFRWPVFEEAVPIDSVSSWTAVQESYDQRNDDCYYIVTLLKEG
                     APVRSFMVKVDVGWAGDDWTTPEFARRLEEEIGRAARRGETNTDYPGPLAR"
     gene            3556..4593
                     /gene="trpD"
                     /locus_tag="PK442_00775"
     CDS             3556..4593
                     /gene="trpD"
                     /locus_tag="PK442_00775"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOO86214.1"
                     /translation="MFAQTLSKVIERKNLESAEMEEAMEALVEGSVPPVQTAAFLTAL
                     RAKGETKEEIGGAVRLLLRKARRVPARRELLMDNCGTGGDCGGTFNISTAAAFVAAGG
                     GIPMAKHGNRAVSSKCGAADVAEALGAEMPDTPERAASCLDAAGLVLLFAPHFHPILR
                     NVAEIRKSLGVRTIFNILGPLLNPAPLTHQVMGVFDPGMAPLVANVLLALGRDRGMVI
                     SGHGGFDELSLSGPNRLVLFSEEGVREAVITPEEAGLARRGDGEFLGGTATENAAIIE
                     DILAGKKGGPRDVVLLNASAVFLVSGMASSWKEGVALASESIDSGRAAEVLEKIRSFS
                     QQREDVIPRSA"
     gene            4609..5367
                     /gene="trpC"
                     /locus_tag="PK442_00780"
     CDS             4609..5367
                     /gene="trpC"
                     /locus_tag="PK442_00780"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOO86215.1"
                     /translation="MILERILHQKMEETNELTAPRRKMSAALKRSGLSVIGEIKRASP
                     SKGIINGGVDPLRQLRLYEQGGADAVSILTDRVFFGGGPEDFSSLRTRTSLPLLRKDF
                     LVSEVQIYESLFLGADALLLIAAALEKERLEALLSLTYSLGMEAIVEVHTEEELFSVL
                     ETDAPILGVNNRNLNDFTVDLRTTARLMDALRSAEGEGSRIVVAESGVRSEEDAGYLA
                     ETGVDAILVGEALMRASNPEETLRSFKRKGRRAA"
     gene            5364..5999
                     /locus_tag="PK442_00785"
     CDS             5364..5999
                     /locus_tag="PK442_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOO86216.1"
                     /translation="MTFVKVCGITRREDIEAAAGLGVDALGFILVPASPRFLPAEEAA
                     ALACTVPEGIRKVAVMVNPSGTEVEAVERTGVFDCIQFHGSETPETLSGCRLETIKAF
                     SVTEEADLENAALFDAASFFLFDSKKRRYDKGVAFDHSMLRGRTFAKPFILAGGLGPD
                     TLRGALREARPFGVDLNSGVEDAPGVKNRVKLAVAVATVRDFDTSERREHR"
     gene            5996..7162
                     /gene="trpB"
                     /locus_tag="PK442_00790"
     CDS             5996..7162
                     /gene="trpB"
                     /locus_tag="PK442_00790"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOO86217.1"
                     /translation="MKTSMYFGIFGGQYVPETLVPALDELETAYLGTLDDASFNGELL
                     ALLHNYSGRPTPLWFAPRITESLGGAKVYLKREDLNHTGAHKINNALGQVLLASKMGK
                     KRIIAETGAGQHGVAVATAAALFGLECVVYMGEEDVHRQAPNVARMNLLGTKVVSVLS
                     GTRTLKDAVNEAIRDWVTNVEDSFYVIGSVMGPHPYPLMVRNFQKVIGEEARAQMLEC
                     EGRLPDQVIACVGGGSNAMGIFHAFVGDPGVALIGVEAAGRGVDTGLHAATISGGKPG
                     VLHGSYSYVLFDEDGQIQPAHSISAGLDYPGVGPEHSHLAATGRATYVSVTDDEAVEA
                     FHRTCRLEGIIPALESSHALAHVYRTAAALPKETIVLVNLSGRGDKDMDTILSL"
     gene            7178..>7366
                     /locus_tag="PK442_00795"
     CDS             7178..>7366
                     /locus_tag="PK442_00795"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOO86218.1"
                     /translation="MNGIAQAFDGKKALITYITAGDPDLDTTAFLLKLLDESGVDILE
                     VGIPFSDPLADGPVIQQAG"
BASE COUNT         1350 a         2367 c         2334 g         1315 t
ORIGIN      
        1 gaatcgctcc acaagcgacc ggacctcccc ggcgcccgag gccagatcgg cggcttcgcg
       61 cgcaacccgt tccgacgcgc cggccgtgtc ctcggcggcg gagcgtatgc cggcgaacgt
      121 cgccgtgagc gaggcaatcc cgctgctcgc cgtctctata cttgcggtca tctccctgga
      181 cgcggcctcc tgttttccag acgcgtcggc aagattgcgc atcagcccgt ccatccggcc
      241 gattcgttcg agcgcgtcct caagatcccg ctgcgcctct tccgctgcgg cgaccgtctc
      301 cttcatgacg ccccccgtct cgttcgtgac gtccatcgcc tccttggtgc cgttctgaag
      361 cgagccgatc aacacctcga cctccttggc ggcgtggttg gactcctcgg cgagctttct
      421 gacctcgtcg gccacgacgg cgaatccccg cccggcctcg cctgcgcgcg cagcctcgat
      481 ggccgcgttg agcgcgagca ggttcgtctg gtcggcgatg gacttgatgg tgttcacgaa
      541 gcggctgatc gactccaccg aggcggagac gctccccatg ctccgggccg actccccggt
      601 tctggtctcc acagtgcgga tcttgccgat gacctgctcg accttctcca ccgcaagacg
      661 gctggtctcc gatgtcgcgg aggcgctttt cgcaccctcc tgggcggaag ccgcggcgat
      721 ttcggccgag gaggcaacgt cgccgacgcc cgactcggtc cggcggatag cttccgcgtt
      781 ctcctccgag agagagaaga ctgtctctat cgagcttttg acctcctgca tggaggcgac
      841 ggtctcctcg gagagctccg caagggattg cgcgctcttc gagaaggcct ccgaacgggt
      901 gaggaggggg cgcagcatct cttccacttt cacccgcagc aggatgagcg cctggatcat
      961 ctccgcgatc tcgtccttcg ccttcgaccg cttgatcatc cagaggtcgg cgggattcac
     1021 tgtgaagtcg agttcgctgc ggcggaccag cacgcggacg acgtcgcgca ggggtttgac
     1081 gatcgaccgg acgacgagca gcatgacgat tccggcgacc agaaccactc cgagcgcgat
     1141 cagcgacgag acgacgccca cacgttgcat tgcttcggcg atgtcggcgg cctgaaggac
     1201 tttttgttga gacgcctcgg tcagcacgcg aagcgaaagc gcgtaccccg taaaggagac
     1261 cgtcgcaatc cccaggaaaa acagcaggag catcggcgcc agaatctttc cgtacaactt
     1321 catacacaaa cccctccctg ataaagactt gtgaatccgc gcaacaacaa tcgtgccgtt
     1381 tccggtcgcg tcggtctcga acgcaccggt acgcgcacca agccggagaa cggtgttttt
     1441 tctatttcac agagtatatt tcgggaaccc tgtcgttgaa gaccgtgagg aaattcctgg
     1501 tttcggcgac ttcgtcgaga tcgatggtgg cgaatcccgc ctcctcgccg ccgccgccct
     1561 cgaagtagat ctctcccttc gggccgagga tcatggagcc gccgccgaaa tgcgtatcgc
     1621 cggaggttcc gcatcggttg caggccacca cgtacatctg gttttcgatg gccctggcgc
     1681 ggagcagcgt ccgccagtgg tccgccctcg cggaaggcca ctgcgaactc acgaaaagca
     1741 cctcggcccc cttgagcgcg tacgtgcgga tccactcgca aaagcggatg tcgtagcaga
     1801 taacgcaccc ggccttcgcg ccttccaggt cgaagagaca ggccttccgg cctcccgtga
     1861 agtacttctc ctcctccatc aggcggatga ggtgcgcctt gtcgtactgc gccacgagct
     1921 tcccctccgg gttgatcgcc tgcgctctgt tcgcgtaccc cttcttcgtc gatgcgagca
     1981 ccgaaccgcc gacgaaccag acgccgtact ttcgggccag ttcgccgaga aaggccgccg
     2041 cctccgctcc ttcggggtcg gccagctcct tcgcccgcga gagcgcgtac cccgtgttcc
     2101 acatctcggg cacgacgacg gcagtcggca gctccgaagg aacgtagaac gaggccatcc
     2161 agtcctcgat attttggcag ttccgctgtt tgtcgccgat ggcaatgtca agctgcagca
     2221 cacccactcg aagtttcatc cgctacctcc tcgatttcgg ccgggaacgc ccctgaccgt
     2281 atagtaaagt cgatgctgtc catttttgaa aaatactgtg aatacgcccc gatatgtttc
     2341 cgctcttatc ctatgaatag tccatgaaat tcttcttgtc aagaggaaat tttctgcgga
     2401 ggccaaaaaa cgcttgtgac cggcgatttt cggtgctaca ctgccattgt tcgggacaaa
     2461 gcccggcgcg gcgcgtcgag ccgccggact gcggcgaagc gcaccggggc gggagtacat
     2521 acacggcgta catccggagg tgtaaaaaat ggcgtatatc acctgcgagg tctgcggcac
     2581 gtacatcgac cacaactaca acacgaccaa gatcatcggc cgctacaact ccatggcgct
     2641 gctctgcgaa tggcatcatg cagtcgtctc ggcgctgatc gcccggcacg gtacggtcaa
     2701 ctggcgggcg ctgggccgcg cgaccagtca ggtccaggaa gagaagccga acgtgctgac
     2761 ggagccggag cgctggctag actatgattc tgttgcgcgt ctgaaggaac tctacgagga
     2821 agagatcgtc cgggatccga cggactggcc tccctacgag tctccgaatg cgaagcgggc
     2881 ggaagaggaa gaggcctccg accgctacct ctaccggtga cgggaatgcc gggggaagag
     2941 atgccttttc gctggcccgt cttcgaagaa gctgtgccca tcgattccgt ctcctcctgg
     3001 accgccgtac aggagagcta tgaccagcgc aacgacgact gctactacat cgttaccctc
     3061 ctcaaagaag gggctcccgt ccgcagcttc atggtcaagg tcgatgtcgg ctgggcaggc
     3121 gacgattgga cgacgccgga gttcgcccgg cggcttgagg aagagatcgg ccgggcggcg
     3181 cgccggggcg agacgaacac ggactacccg ggccccctcg ctcgatgaat gtttccgctt
     3241 attgcgtcgt cttctcaaaa gtttcgcttg acagatcttc cgcaactgtg atatcttctc
     3301 cgcaatcgaa atcggggagg acattctcat gattcgtacc acactcaata agaattggtg
     3361 gtggcgggct tgctagcctg ccgccggtga ccataaagct cgcgatataa gaacaacgcg
     3421 tgttctttaa ggccgtggag gcaggtccct caagggatcg tactctacgg cctttttgta
     3481 ttcccagcgg gggacagagg ccgaacaggc ttctctcccc cgctttctct ttatcagggc
     3541 aaaggaggaa atagtatgtt tgcacagact ctctcgaagg tgatcgaacg gaaaaatctg
     3601 gaaagcgcgg agatggagga ggccatggag gcccttgtag aggggagcgt acctcccgtg
     3661 cagacggccg cgtttctgac ggccctccgg gcgaaggggg agacgaaaga agagatcggc
     3721 ggcgcggtcc ggctgctgct gcggaaggcg cggagagttc cggcgcggcg cgagctgctg
     3781 atggacaact gcggcaccgg cggcgactgc ggggggacgt tcaacatctc cacggcggcg
     3841 gccttcgtgg ccgcgggcgg cggcattccg atggcgaagc acggcaatcg cgcagtctcc
     3901 agcaagtgcg gcgccgcgga tgtcgccgaa gcgctgggtg cggagatgcc cgacacgccc
     3961 gaacgcgccg cctcctgcct cgacgctgcg gggctggtgc tcctcttcgc gccgcacttc
     4021 cacccgatac tgcggaacgt ggccgagata cggaagagcc ttggcgtgcg cacgatcttc
     4081 aacatcctcg gtcccctgct gaatcccgcg ccgctgacgc atcaggtgat gggcgtcttc
     4141 gaccccggca tggccccgtt ggttgcgaac gtcctcctcg cgctggggcg ggatcgcggc
     4201 atggtgatct ccggacacgg cggtttcgac gagctctcac tcagcggccc gaaccgcctg
     4261 gttcttttct cggaagaggg agtgagggag gccgtcatca ctccggaaga agcggggctc
     4321 gcccgcaggg gcgacggcga atttctcggc ggaacggcga cggagaacgc cgcgatcatc
     4381 gaggacatcc tcgcaggaaa gaaggggggc ccccgagacg tggttctctt gaacgcctcg
     4441 gccgtcttcc tcgtctccgg catggcctcc agctggaagg agggggtcgc gctcgcctcg
     4501 gagagcatcg actcaggccg cgccgcggaa gtgctggaaa aaatccgttc cttctcgcag
     4561 cagcgggagg acgttatccc ccgttcggca tgaagggagg ccgttgtaat gattcttgaa
     4621 cgcattctgc accagaagat ggaggagacg aacgagctga cggcgccgcg gcggaagatg
     4681 tcggccgctc tcaagcgaag cggactctcc gtgatcggcg agatcaagcg cgcatccccg
     4741 agcaagggga tcatcaacgg cggcgtcgac ccgcttcgtc aacttcgcct gtacgagcag
     4801 ggcggcgcgg acgccgtctc catcctcacc gaccgggtct tcttcggcgg cggcccggag
     4861 gatttcagct ccctgaggac gcggacaagc cttccgctgc ttcggaagga ctttctggtg
     4921 agcgaagtgc agatctacga gagcctcttc ctcggcgccg acgcgctgct gctcatcgcc
     4981 gcggcgcttg aaaaagagcg tcttgaggcg ctgctctccc tgacgtattc gctggggatg
     5041 gaggcgatcg tcgaggtgca caccgaggag gagctgttca gcgtgctgga gacggacgcc
     5101 ccgatccttg gcgtcaacaa ccggaatctg aacgacttca ccgtcgatct gcggaccacg
     5161 gcgcgcctga tggacgcgct ccgttccgcg gagggggagg ggagccgcat cgtggtggcg
     5221 gagagcggcg tgcgaagcga agaagacgcc ggataccttg ccgagacggg cgtcgacgcg
     5281 atcctcgtcg gcgaggccct catgcgggca tccaacccgg aggagacgct ccgctccttc
     5341 aagagaaagg ggcggcgtgc ggcatgacgt tcgtgaaggt ctgcggcata acgcggcgtg
     5401 aggatatcga agcggctgcc gggctgggcg tcgacgcgct gggcttcatc ctcgtcccgg
     5461 cgagcccgcg ctttctcccg gcggaagagg ccgcggcgct cgcatgtact gtcccggagg
     5521 gcatccgcaa ggtcgccgtg atggtgaacc cgtccggaac ggaggtcgag gctgtggaaa
     5581 gaacgggcgt gttcgactgc atccagttcc acggctcgga gacgccggag acgctttccg
     5641 gctgccgtct tgagaccatc aaggcgttct ccgtgacgga ggaggccgac ctggagaacg
     5701 cggcgctctt cgacgcggcg tcgttcttcc tcttcgacag caagaagcgc cgctacgaca
     5761 agggcgttgc cttcgaccac tcgatgctgc gcggacggac gttcgctaaa ccctttattc
     5821 tcgcgggagg actgggcccg gacaccctgc ggggcgcgct gcgggaggcc cgccccttcg
     5881 gggtcgacct caacagcggg gtcgaggacg cccccggcgt caagaacagg gtgaagctcg
     5941 ccgtcgcagt ggcgacggtc cgtgatttcg acacttccga aaggagagag caccgatgaa
     6001 aacatccatg tacttcggca tcttcggagg gcagtacgtc cccgaaacgc ttgttccggc
     6061 cctcgacgaa ctggagacgg cctacctcgg cacgctggac gacgcgtcgt tcaacggaga
     6121 actgcttgcc ctgctgcaca actacagcgg acggccgacg cccctctggt tcgcgccgcg
     6181 catcaccgag tcgctcggcg gagccaaggt ctacctgaag cgcgaggacc tgaaccacac
     6241 cggcgcgcac aagatcaaca acgcgctcgg ccaggtgctg ctcgcctcca aaatggggaa
     6301 gaaacgcatc atcgcggaga ccggcgcggg gcagcacggg gtggcggtgg ctactgcggc
     6361 ggcgctcttc gggctggagt gcgtggtgta catgggggag gaggacgtcc accggcaggc
     6421 ccccaacgtg gcccggatga atctgctggg gacgaaggtc gtctccgttc tctccggaac
     6481 gcgcaccctc aaggacgcgg tcaacgaggc catccgcgac tgggtgacca acgtcgagga
     6541 ctccttctac gtcatcggct cggtgatggg gccgcacccg tacccgctga tggtccgcaa
     6601 cttccagaag gtcatcgggg aggaggcgcg cgcgcagatg ctcgaatgcg agggacgcct
     6661 ccccgatcag gtgatcgctt gcgtcggcgg cggcagcaac gcgatgggca tcttccacgc
     6721 cttcgtcggc gatcccggcg ttgctctcat cggcgtcgag gcggcgggca ggggagtgga
     6781 cacaggcctt cacgccgcca ccatctcggg cgggaagccg ggcgtgctcc acggctcgta
     6841 ctcgtacgtc ctcttcgacg aagacgggca gatacagcct gcgcactcca tctccgccgg
     6901 gctggactac cccggcgtcg ggccggagca cagccatctc gccgcaacgg ggcgtgcaac
     6961 ctacgtctcc gtcaccgacg acgaggcagt ggaggccttc caccgcacct gccgcctcga
     7021 agggatcatc ccggcgcttg aaagctcgca cgcgctcgcg cacgtctacc ggaccgccgc
     7081 cgccctcccg aaggaaacga tcgtgctggt gaacctctcc ggccgcggcg acaaggacat
     7141 ggacaccatc ctgtcccttt gaaaggagtt acctctaatg aacggtatag ctcaggcctt
     7201 cgacggtaaa aaagcgctca tcacctacat cacggcgggc gaccccgacc tcgataccac
     7261 ggccttcctg ctgaagctgc tcgacgaaag cggcgtggac attctcgaag tgggcatccc
     7321 cttctccgat ccgctggccg acggtcccgt catccagcag gcgggg
//
LOCUS       DAOSWW010000019         7238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_6699_length_7238_cov_3.711820, whole genome shotgun sequence.
ACCESSION   DAOSWW010000019 DAOSWW010000000
VERSION     DAOSWW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6699_length_7238_cov_3.711820"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PK442_00800"
     CDS             complement(<1..287)
                     /locus_tag="PK442_00800"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048480.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOO86219.1"
                     /translation="MAQRVFSGMRPTGKLHLGHLAGALTNWVKLQENYQCFYSIVDWH
                     ALMSEYADSSMIAENCKEVLLDWLASGLDPEKCVIFVQSHVPQHAELHLALS"
     gene            complement(379..1158)
                     /locus_tag="PK442_00805"
     CDS             complement(379..1158)
                     /locus_tag="PK442_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOO86220.1"
                     /translation="MIVGELERRLDEAIPFIWAEGWDNVGLLLGDRSALVRGTAVALD
                     PSIDAMKAALDHSCSVLITHHPLIFHPLKRIDCAEGPGAQIAFAIRHGLTVFSLHTNW
                     DVAPGGVNAAIAEVLSLQEVRPLLPSTTGAWGSGAAGKFPRPLNFLECGAAIRDALHL
                     SRLELHGDMKTSVSTLALCGGSGGTFLPEAFACGAEAYFTADMKYHERLDALDKGVSL
                     FIADHGEMERFSLTALAETVSKATGEKAKILDVSQPAFCMI"
     gene            complement(1220..1296)
                     /locus_tag="PK442_00810"
     tRNA            complement(1220..1296)
                     /locus_tag="PK442_00810"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1260..1262),aa:Ile,seq:cat)
     gene            complement(1377..1559)
                     /locus_tag="PK442_00815"
     CDS             complement(1377..1559)
                     /locus_tag="PK442_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOO86221.1"
                     /translation="MRVSIEKDECIGCGVCAQVCPEVFSMDDDAGVSKVIRPEGGECA
                     KEAADSCPVGCITVEE"
     gene            complement(1626..2426)
                     /locus_tag="PK442_00820"
     CDS             complement(1626..2426)
                     /locus_tag="PK442_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOO86222.1"
                     /translation="MKGQRTERLIRTSARFLTCPSRQLSLTSLAEDFLVSKTVISDDV
                     AIIDEAFSREGLGGIIVDRGRTGGASFVPRIAPEIKDTLLNDLINLLNSEDRFLPGGL
                     VYYSDILFNPHYALRLGYAMASLFSETRPDIVMTSEVKGIPLAIFTAHAMGIPLAVCR
                     FRNRASDGSAVTVHYPTKNGEVKAMYMGTKQLTRGKRVLIVDDFMRGGSTAAGMLLVA
                     KEFGAEVAGTGIFIVSSDPEEKAVSSYKALLRLDGVEKRQPKVSLWEE"
     gene            complement(2433..2720)
                     /locus_tag="PK442_00825"
     CDS             complement(2433..2720)
                     /locus_tag="PK442_00825"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86223.1"
                     /translation="MNEEEAEDERNGILSKLHDKTFCGLLPNDFCTVLLKEHPEYADV
                     FSAFRESGSLGWGITGSGSSAFGLFHQNDGFSRMIRSCRHMQWIRKILFVE"
     gene            complement(3380..3619)
                     /locus_tag="PK442_00830"
     CDS             complement(3380..3619)
                     /locus_tag="PK442_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOO86224.1"
                     /translation="MAGTIRSIREQVALYKGLTVRYRTAKGRRKVEERQGVILETYPN
                     LFTLYVESQDSKVSFSYAELLTREVELELLSGNRN"
     gene            complement(3847..5841)
                     /gene="selB"
                     /locus_tag="PK442_00835"
     CDS             complement(3847..5841)
                     /gene="selB"
                     /locus_tag="PK442_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOO86225.1"
                     /translation="MEGREISLVLGTAGHIDHGKTTLVKAVTGVDCDRLIEEKKRGIT
                     IELGFAPLQLDDGRIVSIVDVPGHERFIRQMVAGASGIDGVLLVIAADEGVMPQTREH
                     LEILSILGVKDGFVALTKTDAVDEDFLELALEDVRDFLTGTFLEGKPIIPVSAIKGSN
                     IGLLREELGRLVDRIRPRPRKGPFFLPIDRSFPMSGFGTVLTGTAYRGELSAGNEGIV
                     LPSGREGRIRSVQVHGADSKVAWAGQRVAVSLAGVSIDEISRGDVLCAKGIYAPTRCF
                     ECELHLLESVPSLKHWQRVRLHIGTSDVTARVSLLESPSIQSGDTVPAQIVTEEDVVC
                     LLDQRFVIRRYSPLETIAGGKVLSPFASKPRGRNARAACIKRIKSLMHAATPAERLAI
                     LIDSAGILHLADAVRLLQETPEEVVRIGSKLRPAGGQTSKQTSSESTGQHPVDEHPIF
                     LQGERKFFLSGRSVARLSSEISVFLQTFHQSHPSQKGALLDEVVLSVLKEFDTRTARS
                     FIDFLVEGGTLVMEEGFIRLSAFTPRDDERFSKDSESLLALCRERGFQPPLLEEAPQA
                     LGMDEKRFSSLLKGMRETGKIAIVSGFLLSFEIEEKLLELLIKEKEGITLARVRDLTN
                     SSRKFILPLLEYIDAKGYTRRAGEVRVLLPSKLPRRRETV"
     gene            complement(5877..>7238)
                     /gene="selA"
                     /locus_tag="PK442_00840"
     CDS             complement(5877..>7238)
                     /gene="selA"
                     /locus_tag="PK442_00840"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOO86226.1"
                     /translation="LLGSPCVEPFFSMIGRESVKSILTEALQSERKELLNGRSDHASC
                     ESVLARAKPLLERRGAKSLIPVVNATGVVVHTNLGRSCLAEEAAEAVLAVARRYNTLE
                     FNLEEGKRGRRTDHVEWLLRRVTGAEAGLVVNNNAGAVLLSLAALCQGKEVIVSRGEL
                     VEIGGSFRIPDIMAFSGVRLVEVGATNRTHLRDYANAVTENTAALLKVHPSNFRMEGF
                     VSSTPREELASLAKDRGLFFIEDLGSGMLIDYSRCGLLGEPTVRDCIEQGVHLVTFSG
                     DKMLGGPQIGGVAGKKQLVDALRSYPLHRALRVDKMTLAAFEATLRLYLRGEEKRIPT
                     VSMLFAESHSLQLRAEALLNTLRSSFPNGQFTLTEVDDAVGGGAFPAEKLPGWGVSVS
                     MEDWGSAGRVQRLLRGAQPPVVAGARENETILHVRTLLPGDEERILEAFRFAETRALR
                     GNE"
BASE COUNT         1689 a         2058 c         1820 g         1671 t
ORIGIN      
        1 gaaagagcaa gatggagctc ggcatgttgg gggacgtgcg actggacaaa aataacgcac
       61 ttctccggat ccagcccgga tgcaagccag tcgagcaaaa cctctttgca gttttccgct
      121 atcatagagc tatccgcata ctcggacatg agggcatgcc aatcgacgat agagtaaaaa
      181 cactgatagt tctcctgaag ctttacccaa ttcgtcaacg ctcctgcaag atgcccgaga
      241 tggagctttc ctgtaggtct cattccactg aaaacacgct gtgccataat caaaccacct
      301 cgaatggaag tatcctctgc gtgcaaggtt tatcaagaat catcatgatt cggtactcag
      361 tcttcagcac caaaagaact atatcataca aaatgcaggc tgtgaaacat ccaggatttt
      421 tgccttttcg cccgttgctt ttgaaactgt ctccgcgagc gctgtgagag aaaaacgctc
      481 catttctccg tgatcggcaa tgaaaagaga gactcctttg tcaagagcgt caagacgctc
      541 atggtatttc atatccgctg tgaaatacgc ttcagcgccg catgcgaacg cctccggcaa
      601 gaacgttccg ccggagcctc cgcacagagc cagcgtcgac acgcttgttt tcatatcgcc
      661 atggagttca agcctggaga gatgcagcgc atcccgtatt gcagctccgc actccagaaa
      721 gttcagaggt cgtggaaact ttccggctgc cccgctcccc caggcgcccg ttgttgaagg
      781 aagaagcggc cggacttcct gcagagagag aacctctgca atagcggcat tcactccgcc
      841 gggagcaaca tcccagttcg tgtgaagcga aaataccgtc aagccgtggc gtatggcgaa
      901 agcgatctgc gcgccgggcc cctctgcgca gtctatccgt ttcagcgggt gaaagatgag
      961 cgggtggtgt gtgatcaaca cggagcaaga gtgatcgagc gccgccttca tggcatcgat
     1021 cgacggatca agagcaacgg ccgtgccgcg aacgagggcc gaacggtctc ccaggagaag
     1081 accgacattg tcccatcctt cagcccatat gaaaggaatc gcttcgtcta gcctgcgttc
     1141 aagttctcca acgatcatag agcatcacct tccagaaaaa taaaaaaacc gtttccccgg
     1201 aaggaaacgg caatgtacgt ggtgggccta cctggactcg aaccaggaac cttccggtta
     1261 tgagccggtg gctctaacca cttgagctat aggcccttca tgaagcgttg ctcattatac
     1321 gcccgaggaa caagaaaaag caagcgcata ccgctttttc gaaaaagtgg gccttgtcac
     1381 tcctctacgg taatacaccc aaccggacag ctgtcggcgg cttcttttgc acactcgccg
     1441 ccttccggcc gtattacctt ggacacgcct gcatcatcgt ccatggaaaa tacctccgga
     1501 cacacctgcg cacacacgcc gcatccaata cattcgtctt tctcaatgga aacacgcatg
     1561 gaagatgtgg tcacctcctt ccgtctacgg gattgtatat cagcggaaag atatcgtcaa
     1621 atcccttact cttcccacaa ggaaactttc ggctgtctct tctcaacgcc gtcaagcctg
     1681 aggagcgcct tgtacgaaga gactgctttt tcctcagggt cggaagagac gatgaatatt
     1741 ccagtccctg ctacttcggc gccgaattcc tttgcgacga ggagcatccc ggccgcagtg
     1801 cttccgccgc gcataaagtc gtcgacaatg agcactctct tcccgcgggt taattgtttc
     1861 gttcccatgt acatagcctt gacctcgccg ttctttgtcg ggtagtgaac tgtcaccgcc
     1921 gacccgtcgc ttgccctgtt acgaaaccgg cagaccgcaa gcgggatgcc catcgcatgg
     1981 gcagtaaaaa tagcgagcgg aatgcccttt acttcggatg tcatgacaat gtccggacga
     2041 gtttcactga agagcgaggc catcgcatag ccgagacgaa gagcgtagtg cggattaaag
     2101 agtatatcgc tgtaatacac aagcccgccg ggcaggaaac gatcctcgct gttcaacaga
     2161 ttaatgaggt cgttcaggag cgtatccttg atttcgggag ctatgcgcgg tacaaatgaa
     2221 gctcctccgg ttctccccct gtcgacgata atgccgccca ggccttccct tgaaaaagcc
     2281 tcgtcaatga tcgcgacgtc atcgctgatg acagtctttg agacaagaaa atcctcggcc
     2341 agagatgtca gcgagagctg tctcgaaggg catgtgagaa atcgtgccga agtacggatc
     2401 aatcgttcgg ttcgttgtcc tttcattcat catcactcca caaataagat tttgcgtatc
     2461 cattgcatat gcctgcagct ccgaatcatt cgcgaaaaac cgtcattttg atgaaacagc
     2521 ccgaaagccg agctgccgct tcctgtgata ccccatccaa gcgatccgct ttcccggaat
     2581 gcgctaaaaa cgtcggcgta ttccgggtgc tctttcagaa gaactgtgca gaaatcattc
     2641 ggcagaagcc cgcaaaacgt tttatcatgc aattttgaga gaataccatt cctctcatct
     2701 tcggcttcct cctcgttcat ggggaaaaaa ccgccgtatt cggctgtgag gagagaaaag
     2761 gctcttgccg tcgacatact ccatgaggga atgacaacga ctgcgtgaaa gggcggcata
     2821 atcccggaga gaggttccac acgttcacct ctgcctccga cgaaagccca tttctctgcg
     2881 ttcaaaaaaa acggaacgtc ggacccaatc tcttcgcctc gaattccttc attctcttca
     2941 cgctgagagt tcagccaaga aatcagagct gcggcgtttc cgcttccccc gccgaggccg
     3001 ctcccagggg gtatgacctt atgaagccgt atgcgcagag gatccactcc tctttttttt
     3061 cgcgccgcag aaagaacttc ttcaaggatg tttcttccgc gaatcatttc tcctgttacg
     3121 gagattatgt cctttacatt atgaccataa tcaggttcaa ttgtcaatga ttcaattgcg
     3181 ggaagcgcat aaaaaagaga acgaagttca tgataaccgt catcacgttt tctggtgact
     3241 cgaagcgtga tgttcacctt cgccgcggat tgcagacagt gaatcaaaag gagcagctcc
     3301 ttccttgaat aattaacaaa aagtatgccc ctccacgggc gacgtggcgg ggcatacttt
     3361 taacataagc agcagagact taatttctgt ttccggacaa gagctccaac tcgacttcgc
     3421 gagtcaaaag ctccgcataa ctgaaggaaa ccttgctgtc ctgcgattcg acgtaaagag
     3481 taaagagatt cggataggtt tcaaggatga cgccctgacg ttcctcgact ttgcgacgtc
     3541 ctttggctgt ccggtaacga accgtcagtc ccttgtacaa tgccacttgt tctcggatcg
     3601 atcgaattgt tcctgccata cgggtcactc ccagcaactc atcattacaa gaatgttatc
     3661 acaaatactg aaaaatatca aatatcatca ggcccgattg tgacgggact gcaagccttc
     3721 tcctgtatgg actatttgcc ttttctccgg cgaataccgt agagcgtcac acgagaaaag
     3781 tcgcttggcg gcatgtcgtt cgcagccggt accgtcctct ccgggtggcg ttccgtattt
     3841 catccttcag acggtttctc tccttcgcgg gagttttgag ggaagtaaaa cccggacttc
     3901 tcccgctctc ctcgtgtacc ccttcgcatc gatatactcc agcaggggaa ggatgaactt
     3961 tctggagctg ttcgtcagat cccgaaccct tgcaagcgtg atgccttctt tttccttaat
     4021 gagaagttcg agcagcttct cttctatttc gaacgagaga aggaagcccg aaacgatggc
     4081 tatcttgcct gtttctcgca ttcctttgag gagcgaggaa aaacgttttt cgtccatgcc
     4141 aagagcctga ggcgcctctt caagaagcgg aggctggaat ccacgctccc tgcagagcgc
     4201 aagaagcgat tccgagtctt tggaaaagcg ttcatcgtca cggggagtaa aagcgctcaa
     4261 gcgaataaac ccctcctcca tgaccagtgt tccgccttca acaaggaaat cgataaaaga
     4321 gcgcgccgta cgcgtatcaa actccttgag cacgctgaga acgacctcgt cgagcagagc
     4381 gcctttctgc gaggggtgcg attggtggaa tgtctgtaag aaaacggaaa tttccgagga
     4441 gagacgagcg acgcttcgcc ccgagagaaa gaactttctc tctccctgga gaaaaatcgg
     4501 gtgctcatcc actggatgct gtcccgttga ttcggaagac gtctgtttgg aggtctggcc
     4561 gcctgcggga cgaagctttg aaccgatacg gactacctct tccggggttt cttgaagcaa
     4621 ccggaccgcg tcggcaagat ggagtatgcc tgccgagtcg atcagaattg caaggcgttc
     4681 agcgggggtc gcagcgtgca taagcgactt gattcgtttg atacaggcgg cacgtgcatt
     4741 tctccccctt ggtttcgagg cgaagggtga aaggactttt cctccagcga tcgtttcgag
     4801 cggactgtac ctgcggatga caaatcgttg atcaagcaga cagactacgt cctcttccgt
     4861 gactatctgt gcggggacag tgtccccgct ctgtatcgac ggcgactcga gaagggatac
     4921 tctggccgtc acgtctgagg ttccgatatg gagtcgaacg cgttgccagt gtttcagcga
     4981 cggcacgctc tcaagcaggt gcagctcgca ttcaaaacag cgtgtcgggg cgtagatgcc
     5041 tttggcacag aggacgtctc cgcgcgatat ctcatcaatg gaaacgcccg cgagacttac
     5101 ggctactcgt tgccccgccc aagcaacctt ggaatcggct ccgtgtacct ggacgctgcg
     5161 gattcttcct tccctgccgg acgggagcac gatcccttcg tttccggcgc tcagctctcc
     5221 tctataggcg gtgcctgtga gcaccgttcc aaagcctgac atcgggaatg atctgtcaat
     5281 ggggaggaaa aaaggcccct ttcgcgggcg gggccgaatc ctgtcgacga ggcgccccag
     5341 ctcctctctc agaaggccga tgttgctccc cttgattgcc gagactggaa taataggttt
     5401 tccctctaaa aacgttccgg tgagaaaatc gcgcacatcc tccaaagcaa gttcaagaaa
     5461 atcctcgtcg accgcgtcag ttttcgtgag cgcaacaaaa ccgtccttca ccccgagaat
     5521 actgagtatt tccaagtgct ctctcgtctg cggcatgacg ccctcgtctg cggcgatcac
     5581 gagaagaacg ccgtcgattc cggaagctcc cgcaaccatc tggcggatga accgttcgtg
     5641 ccccggtacg tccacaatgc ttacaatacg gccgtcatcc agttgcagag gagcaaaacc
     5701 aagttcaatc gtgatgccgc gctttttttc ttctatcaat cggtcgcagt caacgcccgt
     5761 cacggccttt acgagcgttg tcttcccgtg gtctatatga ccggcagtac ccagcaccag
     5821 agagatttct ctgccttcca cattcttcat cctctctcca tcttcgttgc gctctcctat
     5881 tcgttgcccc gaagagctct tgtttctgcg aaacgaaacg cttcgagaat ccgttcttcg
     5941 tcccctggaa ggagtgtacg cacatgaagt atagtttcgt tctcacgtgc tcctgcaact
     6001 accggaggtt gtgctcccct aagaagacgc tgtacccttc ctgcactccc ccagtcttcc
     6061 atggaaacgg aaacgcccca gccgggaagc ttctccgccg gaaatgcgcc tccgcctacc
     6121 gcatcgtcca cctccgtcag cgtaaactgc ccgttcggga aggacgaccg aagggtgttg
     6181 agcagagctt ccgccctcaa ctgtaaggaa tgcgattcgg cgaagagcat tgataccgtg
     6241 gggattcgtt tttcttctcc ccgcagatag agccgcagcg tggcctcgaa tgccgcaagg
     6301 gtcattttgt ccacccggag cgcgcgatgc agcggatagg atctcagggc gtcaacgagc
     6361 tgtttctttc ccgccactcc tccgatctgc gggccgccaa gcatcttgtc gcctgagaag
     6421 gttacaaggt gcactccctg ttcaatacaa tctctcactg taggttcccc caacagcccg
     6481 catcgggaat aatcgatcaa catcccgctg ccgaggtcct cgatgaagaa aagtccccga
     6541 tcctttgcga gagaagccag ttcttcgcgc ggcgtagaag aaacaaaacc ctccatgcgg
     6601 aaatttgaag gatgtacctt gagcagcgcc gccgtattct ccgttacagc gttcgcgtag
     6661 tcccgcagat gcgttctgtt ggttgcgccg acctctacaa ggcgaacgcc ggaaaatgcc
     6721 atgatatccg ggatacggaa tgatccgcct atctccacga gttctccccg ggagacaatc
     6781 acttctttcc cctgacagag cgctgcaaga gagagaagga cggctcctgc gttgttgttg
     6841 acgacgagcc ccgcttctgc gccggtgacg cgccgaagaa gccattctac gtggtcggtt
     6901 cgccgtcccc tttttccttc ttccaaattg aattcaagcg tgttgtagcg gcgcgctact
     6961 gccagcacgg cctctgccgc ttcctccgca agacaggaac ggccgaggtt ggtgtgaaca
     7021 acaacccctg tggcgtttac tacggggatt aaacttttcg ctcctcgtcg ttccaaaagg
     7081 ggtttcgcgc gcgcgagcac tgactcgcag gaggcgtggt cagaacgtcc gttgagtaat
     7141 tcttttcttt cggactggag cgcttctgtg agtatggact ttacggactc gcgcccgatc
     7201 atcgaaaaga aaggttccac gcacggcgaa ccaagaag
//
LOCUS       DAOSWW010000020         7076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_6901_length_7076_cov_3.932773, whole genome shotgun sequence.
ACCESSION   DAOSWW010000020 DAOSWW010000000
VERSION     DAOSWW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6901_length_7076_cov_3.932773"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..478
                     /gene="rplL"
                     /locus_tag="PK442_00845"
     CDS             98..478
                     /gene="rplL"
                     /locus_tag="PK442_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOO86227.1"
                     /translation="MTRDEMIQAIENMTVLELSELVKALEEKFGVSASAPAMAMPMMA
                     MGAAPAAAAEEEKTEFDVIYKAPGANKINVIKVVRELTGLGLKEAKELVDNPPKAVKE
                     AVSKEEAEEIRKKLAEAGADVEVK"
     gene            551..1390
                     /locus_tag="PK442_00850"
     CDS             551..1390
                     /locus_tag="PK442_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO86228.1"
                     /translation="MIDCHVHVYPPEIIRDAAVIGKNEPYFELLSTGKVHKWATAEDV
                     IAAMDRDGIDVSWIFGFAFKDPGLCALCNEYVIEAVRKYPTRLKGLAVVPPCAPGAGN
                     EIARCREQGLVGVGEIFPGGQDFDISDVRQTWRLAGAVAEAGMFLLVHSAEPVGHDYP
                     GKGNTGPREAAEFCLHHPEVEVVFAHFGGGLWMYELMPEMQLALSNAYYDSAAWPWLY
                     EPSLLRAMEAAGVLRKLFYGSDFPILGYPKYEKMIAASGVERGEVEKFLHHNAFDFMA
                     KRC"
     gene            1442..1516
                     /locus_tag="PK442_00855"
     tRNA            1442..1516
                     /locus_tag="PK442_00855"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1474..1476,aa:Val,seq:tac)
     gene            1540..1616
                     /locus_tag="PK442_00860"
     tRNA            1540..1616
                     /locus_tag="PK442_00860"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1574..1576,aa:Asp,seq:gtc)
     gene            1633..1708
                     /locus_tag="PK442_00865"
     tRNA            1633..1708
                     /locus_tag="PK442_00865"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1666..1668,aa:Phe,seq:gaa)
     gene            1732..1806
                     /locus_tag="PK442_00870"
     tRNA            1732..1806
                     /locus_tag="PK442_00870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1764..1766,aa:Gly,seq:gcc)
     gene            1817..1890
                     /locus_tag="PK442_00875"
     tRNA            1817..1890
                     /locus_tag="PK442_00875"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1849..1851,aa:Cys,seq:gca)
     gene            1907..1982
                     /locus_tag="PK442_00880"
     tRNA            1907..1982
                     /locus_tag="PK442_00880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1940..1942,aa:Asn,seq:gtt)
     gene            2143..2307
                     /locus_tag="PK442_00885"
     CDS             2143..2307
                     /locus_tag="PK442_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86229.1"
                     /translation="MFKKLVYMAGKPFWLLPEGSLLDVEQRNGRIVDPVVFRKKNDDR
                     INENTHLSMN"
     gene            2647..6369
                     /locus_tag="PK442_00890"
     CDS             2647..6369
                     /locus_tag="PK442_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO86230.1"
                     /translation="MSLFNAINKTYFNAVLVFLCLLLLTFFYAYYRRTKNFSAWSTLF
                     LLVSGSMVVYLFYQGGTKGAGLLWAYIFPFLAFQFKGYKAGLRFVAVLYAVLAGVFAS
                     ALTGHAPLHFSPLFMIVYFSVHITISGFLFFYEKRRTITERIIFESREKYKALFDNFS
                     VGVAMISPEFELLELNGIARKWFDRSPEKQHSFCYEFLDSEQKLGVCEDCPLHSVMES
                     KHTATQYRKKMTVDGIRNFKIVCSPMFDKERNISAVIETLEDVTEIQRAREFLEESEN
                     RFRTLFEQVPFISVQGYDKERNVIFWNSASELLYGYPAKEALGKRLEDLIIPAEKIQE
                     HVEAISAWIQGGPPLPPSEVMLKRADGSSVHVFSSHVLIRNVRGEKEFYAIDIDITER
                     KRLEEHLSSNEEKYRLLVETAQEAICVAQDERLVYFNPTVSDMLGYSDSELSSMHFLE
                     MVYEDDRELLLQNYRKRLAGSGVEQSYEIRLLRKDGTVAWVELKATLIEWNGSPATLV
                     FLNDITTQKAALRELMETNRRLELAIERTREFATKADAANRAKSDFLANMSHEIRTPM
                     NGVLGMAGLLLDTELNREQRRCAEAVRASAESLLGVLNDILDFSKIEAGKLDLERLRF
                     DLFVMLDDFAAGMAVRAHQKKLEFICAPDPDVPSRLLGDPGRLRQILTNLVGNAIKFT
                     SKGEVVVRVSLEEESEQSALLRFSVRDTGIGVPDEIKESVFEKFIQGDSTTTRRYGGT
                     GLGLSISKQLVEMMGGRIGVESPPKLSAENGLSHWSEAGPGSDFFFTVRCEKQPSEVE
                     SGIVVQGELSGVRVLIVDDNATNREIVRAHLVSRGMRPFEAQDGASALRLLEEAVEQG
                     DPAALALVDMNMPEMDGETLGRKIREDGRFSQTRLVMLTSLADRGDAKRFAEAGFSAY
                     LPKPVRARELLGVLALVHARGEDVSPDFFATRHSLNELSSAHPRPFAGKGYRILVVED
                     NSTNRQVALGILGKFGVEACSVENGEKAVEEVGRTAYDLLLMDVQMPVMDGLEATRRI
                     RSLPPQSTGPFVPIIAMTAHAMRGDRERCLEAGMNDYISKPVTPDVLADVLERWLGTW
                     EGRHPVPEPDVSPFALPDAKGAFEETNREPDAEALIWDRNVLLERLLGDEEMVREIVH
                     GFLEDIPCQMRALEEAIRACDCVAVRERAHAIKGAAASIQAEALRAASFDVEKAGKSE
                     EADSMPSLFAEMEHCFDLLKQKMNSVEVVRREDTDS"
     gene            6350..>7076
                     /locus_tag="PK442_00895"
     CDS             6350..>7076
                     /locus_tag="PK442_00895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO86231.1"
                     /translation="MRILIAEDDLTSRKILAGVLRKIGHEVVETVDGLAAWTELQKTD
                     GPKLAILDWMMPEMEGLDVVRKVRALDIEKPPYIIMLTTRGEKSDIIAGLDAGANDYL
                     SKPFDTGELQARVEVGRRMVELQDALVESRKALAYEASHDPLTKLANRRAILEYLERQ
                     MVSASENGDILTAGMCDIDHFKRVNDTWGHQVGDDVLCELAHLLRESAEGRGAAGRMG
                     GEEFLLVLRMKAGGDYMAESAAIC"
BASE COUNT         1594 a         1792 c         2091 g         1599 t
ORIGIN      
        1 aacagaagga agacgtcgca tagtttttgt accggaaaca cttcgcatac gcagtatgcg
       61 atacacaaga tgatatatat ccaaggagga aaaaagaatg acccgcgatg aaatgattca
      121 ggctatagag aacatgacag tgcttgagct ctccgagctc gtaaaggctc ttgaggagaa
      181 gttcggagtt tccgcttccg ctcccgcgat ggcgatgccg atgatggcga tgggcgcagc
      241 ccccgccgct gccgccgagg aagagaagac cgagttcgac gtaatctaca aggcgccggg
      301 cgccaacaag atcaacgtga tcaaggttgt ccgcgaactc acaggccttg ggctcaaaga
      361 agccaaggaa cttgtcgaca acccccccaa ggcagtcaaa gaagcagtca gcaaggaaga
      421 ggcagaggag atcaggaaga agctcgccga ggccggagcc gacgtcgagg ttaagtagtt
      481 cgcctttcct tttttctggt ataataaaga cggtgcgaaa gcatcgtctt ttttttatgg
      541 ggggtatctc atgatcgact gtcatgtaca cgtctatccg cccgagatca tcagggatgc
      601 ggcagttata gggaaaaatg aaccgtattt cgaactcctc tcgacgggca aggtacataa
      661 atgggccacg gcggaggacg ttatcgccgc gatggacagg gacggcatcg acgtctcgtg
      721 gattttcggt ttcgccttca aagatcccgg actctgtgcg ctctgcaacg agtatgtgat
      781 cgaagctgta cgaaagtacc cgacacgcct gaaggggctt gccgtggttc ctccgtgcgc
      841 tccgggcgca gggaacgaaa tcgcccgatg cagagaacaa ggtcttgtag gcgtcggaga
      901 aatatttccc gggggccagg atttcgatat ctccgatgtc cgccagacgt ggaggcttgc
      961 cggagcagtt gccgaagcgg ggatgttttt gcttgttcat tccgccgagc ccgtcggcca
     1021 tgattacccc ggaaagggaa acaccggtcc gcgggaagcc gcagagtttt gtttgcatca
     1081 tcccgaggtc gaagtggttt ttgctcattt cggagggggg ctctggatgt acgaactcat
     1141 gcccgagatg caacttgcgc tttccaacgc atactacgat tcagcggcgt ggccttggct
     1201 ctatgagccc tccttgcttc gcgcaatgga agccgccgga gtgctccgta aactgttcta
     1261 cggctccgat ttcccgatcc tcgggtatcc taaatacgag aaaatgattg cagcgtccgg
     1321 tgttgaaaga ggagaagttg aaaaatttct acatcacaat gcgttcgatt ttatggcgaa
     1381 aaggtgttga cagaaaccct catcccttat atactatccc tcgcgttgct ggaacggacg
     1441 cgggcggata gttcagcggt agaacacctg ccttacacgc agggggtcac aggttcgaac
     1501 cctgtttcgc ccaccaaggc ttcaatggat gtactatgcg gggtcgtagc tcagtcggtt
     1561 agagcgtcgg cctgtcacgc cgaaggtcgc gagttcgagt ctcgtcggcc ccgccattta
     1621 caatccaatt gaggcgggat agctcagatg gtagagcagc ggactgaaaa tccgcgtgtc
     1681 cccagttcga ttctgggtcc cgccaccatc ttcgcattat tgaaaaagga tgcggaagta
     1741 gctcaggggt agagcacaac cttgccaagg ttggggtcgc gggttcaaat cccgtcttcc
     1801 gctccatttg ttgtacggcg gcatagccaa gtggtaaggc agaggactgc aaatccttta
     1861 tccccagttc gaatctgggt gccgcctcca ctacaccaga gatcattcct cggtagctca
     1921 atcggcagag cgggtggctg ttaaccacta ggttcgaggt tcgagtcctc gccgaggagc
     1981 cagaaagttt ccaggccatt ctggattttt ccggaaggcc ttttttattt gagtcttgcc
     2041 gcgcgcgtac ttccctcgaa gagagccctt accgaagtct tttttcggcg cgctggatga
     2101 tcggaatata gaatagaaga aaagaagaag agaggtggat atatgtttaa aaagttagtg
     2161 tacatggctg gaaaaccatt ctggcttctg cccgagggca gtctgctcga cgtcgaacag
     2221 cgtaacggcc gtatcgtcga tccggttgtt ttccgcaaga agaacgacga cagaatcaac
     2281 gagaacactc atctgagtat gaattagtcc ttcccaacgg gaagtgcacc taatacagat
     2341 cggctgtaca ggctccggta atccggggcc tgtttttttt ggtctctttc gggaagtagc
     2401 cggccggtca tttcgcttcc gcaggttcgc ccggcacatc ttcgacgctc gttacgaaac
     2461 aaccttctct tcaaattcat gatacaatat aataacaatt catctgttgt ttttctttgc
     2521 ggctccgtat tttgaatttg gttctcacga ggtcgagcta tggaccagat gcagtcgggg
     2581 aagtcccgta acaatgacag gacggtacaa agtgtctccg cgcttttgtg ttccataagc
     2641 gcggcgatgt cgttatttaa tgccatcaac aagacgtact tcaacgccgt gcttgtattc
     2701 ctctgtcttt tgctgctgac ctttttttat gcgtactatc ggcggacgaa aaatttttca
     2761 gcctggtcaa ctctgttttt attggtctcc ggctccatgg tcgtctatct cttttaccag
     2821 ggagggacga aaggcgccgg tcttctttgg gcctatatct tcccgttcct cgcgtttcaa
     2881 ttcaaagggt acaaggcagg cttgcgcttt gtcgctgttc tctacgcagt gctggccggc
     2941 gtgttcgcgt cggctttgac tggacatgcc cccctccact tttcccctct tttcatgatc
     3001 gtctatttct ccgttcatat aacgatctcc ggttttcttt tcttttacga aaagcggcga
     3061 acaatcaccg agcggatcat tttcgagagc agggagaagt ataaggcgct cttcgacaat
     3121 ttttcggtgg gggtggccat gatttctccg gagtttgagc tcctggagct gaacggtatc
     3181 gcgagaaaat ggtttgatcg ctctccggaa aagcagcatt ctttttgcta cgaatttctg
     3241 gattcggagc aaaaactcgg cgtctgcgaa gactgccctc tgcactcggt aatggagtcg
     3301 aaacatacgg cgacgcagta caggaaaaag atgacagtag acggcatccg taatttcaag
     3361 atagtctgca gcccgatgtt cgacaaggaa aggaatattt ccgcagttat cgaaactctc
     3421 gaggacgtta ccgaaattca acgggcgcgc gaattccttg aggagagcga gaacaggttc
     3481 cgcactcttt ttgaacaggt cccatttatt tccgtacagg ggtacgacaa agagagaaac
     3541 gtgatcttct ggaacagcgc gagcgagctc ctctacgggt accctgcgaa agaggctctt
     3601 ggaaaacgac tggaggatct cattataccc gccgaaaaaa tacaggagca tgtcgaagcc
     3661 atctcggcat ggatacaagg aggccctcct cttcctccct ccgaggtcat gctgaagcgg
     3721 gctgacggca gctccgtgca tgtcttctcc agtcacgtgc tgatccggaa tgtacgagga
     3781 gagaaggagt tctacgctat tgatattgac ataaccgaac ggaagcgtct tgaggagcat
     3841 ttgagttcga atgaggagaa gtaccgtctt ctggttgaaa ccgcccagga ggccatctgt
     3901 gtcgcccagg acgaacgact ggtctacttc aaccccacgg tgagcgatat gctcgggtat
     3961 tccgacagcg agctttcttc gatgcacttt cttgaaatgg tctatgaaga cgacagggag
     4021 ctgctgctgc aaaactatcg aaagcgcctt gcagggagcg gcgttgagca gagctacgaa
     4081 atccgtctgt tgcgaaagga cggcacagtg gcgtgggtgg agctgaaagc gaccctcatc
     4141 gagtggaacg gcagcccggc cacgcttgtc ttcctcaacg acatcacgac gcagaaagcc
     4201 gctctgagag agcttatgga gacgaaccgc aggctggagc ttgccatcga gcgaacaagg
     4261 gagttcgcca caaaggccga tgcggcgaat cgggcgaaaa gcgattttct tgccaacatg
     4321 agccacgaaa tacgcacgcc gatgaacggc gttctcggga tggcgggttt gttgctggat
     4381 accgagctga accgagagca gcggcggtgt gcagaggctg tgcgcgcaag cgccgaatcg
     4441 cttctcggcg tgctcaacga tatccttgat ttctccaaga ttgaagccgg caagcttgat
     4501 cttgagcgcc ttcgattcga tcttttcgtt atgcttgacg attttgccgc cggcatggct
     4561 gttcgtgcgc accaaaagaa gctcgagttc atctgtgcgc cggacccgga cgttccatcc
     4621 cgcctcttgg gcgatccggg gcgtctccgc cagattctta cgaacctcgt tggcaacgcc
     4681 atcaagttta catccaaagg ggaggttgtc gtgcgcgttt ccctggagga ggagagcgaa
     4741 cagagcgcgc ttctccggtt ttccgtgagg gatactggaa tcggcgtgcc cgatgaaata
     4801 aaagagagcg tctttgaaaa gtttatccag ggagactcca cgacaacccg ccgttacggg
     4861 ggcacagggc tcgggctttc catctcgaaa cagctggttg agatgatggg aggaaggatc
     4921 ggcgtggaga gccccccgaa gctttctgct gagaacggtc tttcccattg gagcgaggcg
     4981 gggcccgggt ccgacttctt ttttacggtc cgatgcgaaa aacagccttc tgaagtggag
     5041 tccggtattg tcgtacaggg agagctgagc ggagttcggg tgttgatcgt tgacgacaac
     5101 gcaaccaatc gggagatcgt ccgcgcgcat ctcgtctccc gcggtatgcg tcctttcgag
     5161 gcgcaggatg gagcctccgc cctccggttg cttgaggagg ctgttgagca gggcgatccg
     5221 gccgctctcg ccctcgtcga tatgaacatg ccggaaatgg acggcgaaac gctgggaaga
     5281 aaaatccgtg aggatgggcg tttctcacag acgcggctgg ttatgctcac ctcgcttgcg
     5341 gacagaggag acgccaaacg ttttgcggag gccggatttt ccgcctacct tcccaaacct
     5401 gtccgtgccc gcgaactcct tggagtactt gcgttggtgc atgccagggg agaagatgtc
     5461 tctccggact ttttcgccac acgtcactcg ctgaacgaac tttcatcggc gcacccccgg
     5521 ccttttgccg ggaaggggta ccgtatcctc gtcgtggagg ataattccac caaccggcag
     5581 gttgcgctcg ggatactcgg aaagttcggc gtagaggcct gttccgtcga gaacggagaa
     5641 aaggccgtcg aggaggtcgg cagaacagcg tacgatctgc tccttatgga tgtgcagatg
     5701 cccgtgatgg acggacttga ggctacccgg cggattcgtt cccttcctcc gcagagcacc
     5761 ggtcctttcg tgccgatcat cgcgatgaca gcgcatgcaa tgcgtggaga cagagaacga
     5821 tgtcttgaag ccgggatgaa cgactatatc tccaagccgg tgacgcccga cgtacttgcg
     5881 gatgtccttg aacgctggct tggtacatgg gaagggcgcc atcccgttcc agagccggat
     5941 gtatcgccgt tcgcgctccc cgatgcgaag ggcgccttcg aagaaaccaa cagggaacct
     6001 gacgcggagg ctctgatctg ggacagaaac gttcttcttg agcgcttgct gggggacgaa
     6061 gagatggttc gggagatagt gcacggcttt cttgaagata ttccgtgcca gatgagagcg
     6121 cttgaagagg cgataagggc ctgcgattgc gttgcagtga gggagcgggc gcatgctata
     6181 aagggagcgg cggcgagtat tcaggccgaa gctctgcggg ctgcgtcgtt cgatgtggaa
     6241 aaggccggga agtcggaaga ggcggattcg atgccttctc tcttcgcgga gatggagcat
     6301 tgtttcgatc tcttgaagca aaagatgaac tccgtggagg tagtgcgccg tgaggatact
     6361 gatagctgaa gacgatctga catctcgcaa aattctcgca ggagtgctcc gtaagatcgg
     6421 ccacgaggtc gtggagacgg tggatggttt agccgcatgg acggagctgc aaaaaacaga
     6481 cggcccgaag ctggcgatcc tcgactggat gatgccggag atggaggggc tggatgtcgt
     6541 acgaaaggtg cgcgccctgg atattgaaaa accgccgtat attatcatgc tgactaccag
     6601 gggagagaag tcggacatta ttgccggcct ggacgccgga gcgaacgatt atctctcgaa
     6661 gccgttcgat accggtgaac ttcaggcgcg cgtcgaggtg gggcgccgta tggtcgaact
     6721 tcaggacgcg cttgtcgaaa gcagaaaggc gcttgcctac gaagcctccc atgatccgct
     6781 gaccaaactg gccaaccggc gggctattct ggagtacctg gagcggcaga tggtctccgc
     6841 gagtgagaac ggcgatatcc tgacggcggg aatgtgcgat atcgatcact tcaagagagt
     6901 gaacgatacg tgggggcatc aggtgggcga cgatgtgttg tgcgagctcg cgcatcttct
     6961 gcgcgaaagc gccgagggga gaggggccgc aggccgtatg ggcggggagg aatttctgct
     7021 tgtcctccgg atgaaagcgg ggggcgatta catggcggaa tccgccgcga tctgcg
//
LOCUS       DAOSWW010000021         7065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_6916_length_7065_cov_4.480029, whole genome shotgun sequence.
ACCESSION   DAOSWW010000021 DAOSWW010000000
VERSION     DAOSWW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6916_length_7065_cov_4.480029"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1164
                     /locus_tag="PK442_00900"
     CDS             160..1164
                     /locus_tag="PK442_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOO86232.1"
                     /translation="MLLALCCLWSVPASAAEVFLVSSYHEGDMCGQPQYEAVVEALKQ
                     SGIPELSFRGYYLDSRRRVGKDIDREIDTIIADIRERKPALVFTIDDLAFARLYGEVL
                     RHPSTFLVFTGLNRAVAEYDKGASFLDEKGLPAKNITGVFEYLFMKEQFEMLEVLLQR
                     PLRKVALLHSVDPVGVILKEQIADELSRTPYAGIVEFHAAEDAESALRLADAIGADDS
                     VDAWIPVTMSVKDSEGGFLTMGDLAKEMTARIRKPDLALNVSFTELGFYGGVSVDFYQ
                     MGFQTGSMAAKLLKGLPIREIPVENAGASIIAVNRSRLRELGISIPGDFSAVVDLFLE
                     "
     gene            1196..4462
                     /locus_tag="PK442_00905"
     CDS             1196..4462
                     /locus_tag="PK442_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86233.1"
                     /translation="MTDPVLVFLHLVAAGYLLFLCVLTLLPSAEETSLKGGPLPSAPL
                     FLFNAGWGVALSVRAFLAANSFQSTVYPLAVSLAVGGSLFLIKKKGRTGMLLRALLAG
                     GCASLFLPGTRLPGGALFLLAGGWLFIDRFPLRARLGLSSTGDAPGGARDRAFSMFFL
                     AEGLELLFEGSTPFPVLLSLFFVFTGIFLLARLTFRDRKFPSKVRRALLAGFALYGAL
                     SLVGGVGIARMERSFRESLLREGYRRLEMVRSKFLFFEMMGSALAKTVASDPTLVREG
                     HAPSFASDLQLRLLSRRLGATLVFLLDTEGTVVSTSEKLLEGMNFAFRSYFQDALQGR
                     SGLMYAVGKATMSAGAYFSRPLVDRLGTVLGIVVVKMDLEPVFGELFRTDRVVMHRQG
                     EILSGPKGFESGRLEILQEDFLTLRCMSGEFQGGGVRGPDGRMLPIVSLPLPGGGWEL
                     TKLLDPGSLIRYRRTLFAFYSLTSVLVLLLLLRYTQKNQLIGELKREVREREAAEQAE
                     RAARASAEQTNALLAEERNRAQMLAEQAEAASTAKSSFLANMSHEIRTPLNAVLGMIG
                     LLLDTKLDERQRHYAEVVQTSGDALLSLINDILDFSKIEADRLEMEHIGFNLQTILED
                     TVEMLSQRAEQKGLHFGFFVEDDVPRFLKGDPGRLRQILINLIGNAIKFTPSGEVILN
                     VMLGSEVRETVSIRCTVRDTGIGIPEDRLPALFSAFEQVDASTTRTFGGTGLGLAISK
                     RLTELMGGHIGVESRIGEGSTFWFSIPFPVVPESDVARTADPEFLQEACECEAPMGSR
                     GKILLVEDTPVNQQVAVALLARMGYMADVAGNGEEALTLLDSIPYDLVLMDIQMPVMD
                     GFETTALIRSREASEKRERIPIVAMTAHALSGYREKCIEAGMDDYVSKPILPKELETA
                     LRRHLKKRSAEREEEIAAEEVAEEVKAEKGTENVLETAESSQWEDIPLFDQADGAVRT
                     AGDVVFLGKLLLLFADSNAGEEGRLREFAGRGEWESAKHAAHSLKGSSGNLGLLRLAE
                     QARWTELRLAEYMAGQSELDENALRSAVLSLADLASETMELARRQGTILGKEAEEAPS
                     "
     gene            4535..5434
                     /locus_tag="PK442_00910"
     CDS             4535..5434
                     /locus_tag="PK442_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO86234.1"
                     /translation="MTAPRQESRAAGEPNACRAISGREALRALACIAHLIREIFPGDM
                     SFAVIEDDEYLVYVPGRTIDIKKKPGDKLTEGSAGHRCMMEKRKIVKEFKKEDSPFGF
                     PYVAASVPIFDASGKAVGCVITAENITRQETMRETAELLRASTGQIADSLQTMTGQME
                     EMRAAGEMLNSITSQAVSKVEGTEQVFGVIEGVAKQTNMLGLNASIEASRLGDQGKGF
                     GVVAEEVRKLAVRSSDSARQIKEILEYFKNSTIEINSSTDTLLHIIKEQSRIIETIAA
                     SSEEMASVSAMLEKLSSSLGQGS"
     gene            5553..6491
                     /locus_tag="PK442_00915"
     CDS             5553..6491
                     /locus_tag="PK442_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOO86235.1"
                     /translation="METLRTHALFFLCLALAVALALLPPTAPYAVGMALVLGAAVSNL
                     APGSAPPGLGKMRKLALNSAVVLFGFGLNIRQVISVGGQGFWQTAVSLAVIISLGFFL
                     LRFFKVEAATLKLVTFGTSICGGSAIAAVSSVMKVRDEDIGVAMGVVFLLNAVALVAF
                     PLLASLLPLTPEQYGVWCALSIHDTSSVVGAAAVLGDASLQTATIMKLTRTLWITPIV
                     FLISLRQGEKGKLSIPLFIVFFLLASVTASVLPFAPFFKTLASSGKVLMAVALYIVGF
                     GLHRSVLKKVGAQGMLFGMLLWAVSIVMGYLLASMS"
     gene            6519..>7065
                     /locus_tag="PK442_00920"
     CDS             6519..>7065
                     /locus_tag="PK442_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOO86236.1"
                     /translation="MNLARFPRRRYTEGRTPLEFLERLSAKLGGPEIWIKRDDMLGFL
                     PGGNKTRKLEFLMADALAQGADAVITCGAVQSNHCRLTLAAARKEGLECHLVIEERVP
                     GSYSEQASGNNFLFHLMGVDSIRVVPAKSDMLAEMEKTADELRRAGKKPYVIPGGGSN
                     ALGALGYVACAEEILAQAFDKG"
BASE COUNT         1276 a         2046 c         2290 g         1453 t
ORIGIN      
        1 tagattttat tgcaggttgg tatgcgcctt agcggggtgc gccggatgcg ctgaaaggag
       61 cgtgtgcatg tgactgtttc gtcagttgcg gaaaacgaaa agcgtgcgcg tggttcttct
      121 gttcgggaga gactgtggaa atccgggagg cggcttcttt tgcttcttgc gctgtgctgt
      181 ctgtggagcg ttccggcttc ggcggctgaa gtattcctgg tgtccagcta ccacgagggg
      241 gatatgtgcg ggcagccgca gtacgaagcg gttgtggagg cgctcaagca gagcggtatt
      301 ccggagctct ccttccgtgg gtactatctc gactcccggc ggcgcgtcgg gaaggatatc
      361 gacagggaga tcgacacaat catcgcggac atcagggaac gaaaacccgc ccttgtgttc
      421 acgattgacg accttgcctt cgcccggctg tacggcgagg tgctgcgcca tccgtcgacc
      481 tttcttgtgt tcaccggact gaaccgtgcg gttgcggagt atgataaggg cgcttccttc
      541 cttgatgaga aggggcttcc tgcgaagaat atcaccggcg tctttgaata tctcttcatg
      601 aaggagcagt tcgagatgct ggaagtgctc ctccagcggc cgctgcgcaa ggtggcgctc
      661 ctgcactcgg tcgatcccgt gggcgttatt ttgaaggagc agattgccga cgagctctcc
      721 cgcaccccct acgccggcat cgtggagttt cacgccgcgg aggacgcgga gagcgcgctt
      781 cgccttgccg atgccatcgg cgccgacgac tccgtcgacg cgtggattcc ggtgacgatg
      841 tcggtgaagg actcggaagg cggatttctc accatgggag atctggcgaa ggagatgacg
      901 gcgcgcattc gcaagcctga cctggcgctc aacgtctctt tcaccgagct ggggttctac
      961 ggcggcgtca gcgtggactt ttaccagatg gggttccaga ccggctcgat ggccgccaag
     1021 ctcctgaagg gacttccgat acgggaaatt ccggtggaga acgcaggcgc ttcgatcatt
     1081 gccgtcaaca ggagccgtct gcgcgagctg gggatctcaa ttcccggcga tttttccgct
     1141 gtcgtcgatc tattcctgga atgagtcgtt tttttcggct ccgaaggagg gcgggatgac
     1201 ggatccggtt ctggtgtttc ttcatcttgt cgcagcgggg taccttcttt tcctctgcgt
     1261 cctgacgctt cttccatctg ccgaggagac ctccctcaag ggagggcctc ttccaagcgc
     1321 gcctctcttc ctcttcaatg cggggtgggg cgtcgcgctc agcgtccgcg ccttcctggc
     1381 cgccaattcc tttcagagca cggtctatcc gctggccgtc tcgctggcgg ttggggggag
     1441 ccttttcctc atcaagaaaa aggggcgtac aggaatgctc ctacgggcgc tgctcgcagg
     1501 aggctgcgcg tctctttttc tgccgggaac caggctcccg gggggcgcgc ttttccttct
     1561 ggcgggggga tggcttttca tcgaccgttt cccgctgcgc gcgcgcctcg gcctctcctc
     1621 gaccggagat gctcccgggg gcgcgcgcga cagggcgttc tcgatgtttt ttctcgccga
     1681 ggggctggag ctcctctttg aaggcagcac gccgttcccg gttcttcttt ctcttttctt
     1741 cgtctttaca ggaatcttct tgctcgcccg gctgaccttt cgcgacagga agttcccctc
     1801 caaggtacgg agggcgctgc tcgcgggttt tgcgctgtac ggcgccttgt cgctcgtcgg
     1861 aggcgtcggg atcgcgcgga tggagcgctc gttcagggag tcgcttctcc gcgaggggta
     1921 caggcggctt gagatggtga gaagcaagtt tctcttcttc gagatgatgg gatcggctct
     1981 tgcgaagacg gtcgcctccg accccacgct ggtcagggag gggcacgcgc cgtcgttcgc
     2041 ctcggatctt cagcttcgtc tgctgagccg gaggttgggc gccaccctcg tctttctgct
     2101 tgatacggag ggaacggtcg tctccacctc cgagaaactg cttgaaggaa tgaacttcgc
     2161 cttccgttcg tactttcagg acgctcttca gggaagaagc ggcctgatgt acgcggtcgg
     2221 aaaggccacg atgtccgcgg gggcgtactt ctcccgtccc cttgtggaca ggttggggac
     2281 ggtcctcggt atcgtcgtgg tcaagatgga cctggaaccg gtcttcggcg agcttttccg
     2341 cacagacagg gttgtgatgc atcgtcaggg ggagattctc tcggggccga agggtttcga
     2401 gagcgggcgc cttgaaattt tgcaggaaga ttttttgacg ctgcggtgca tgtccggcga
     2461 atttcagggg ggaggggtgc gaggcccgga tgggcgaatg ctgccgattg tttcgctgcc
     2521 gcttcccggg ggtggctggg agctgacaaa actgctcgac ccgggctctc tcatccgata
     2581 ccgaagaacg cttttcgcct tttattcgct cacctccgtc cttgtgctgc tgctgctgct
     2641 gcgttatacg cagaagaacc agctcatcgg cgagctgaaa cgggaggtcc gcgaacggga
     2701 ggcggcggaa caggcggagc gcgcggccag ggcctccgcg gaacagacga acgcccttct
     2761 tgcggaggag cgcaaccggg cgcagatgct tgcggaacag gctgaagccg ccagcactgc
     2821 gaagagcagc ttcctcgcca acatgagcca cgagatacgc actccgctga acgccgtgct
     2881 tggcatgatc ggccttctgc tcgatacgaa gctcgacgaa cgacagcgcc actacgccga
     2941 agtcgtacag acgagcgggg acgcgctgct ctcgctgatc aacgatatcc tcgacttctc
     3001 aaagatcgag gcggaccgtc tggagatgga gcatatcggg ttcaatctgc agacgatcct
     3061 tgaggatacg gtggagatgc tctcccaaag agccgagcag aaggggctgc atttcggctt
     3121 cttcgtcgag gacgacgtcc cgcgtttctt gaaaggggat cccggacgcc tccgccagat
     3181 actgatcaac ctgataggca acgcgatcaa gttcactccc tcaggcgaag tgatcctcaa
     3241 tgtgatgctc gggtcggagg ttcgtgaaac ggtctcgata cgctgtaccg tccgcgacac
     3301 ggggatcggt atcccggagg atcgccttcc ggcgcttttc agcgccttcg agcaggtgga
     3361 cgcctccaca acccgtactt tcggcggcac agggctgggg ctggccatca gcaagcgcct
     3421 gacggagctg atgggcggac atataggtgt ggaaagccgc atcggcgagg gctccacctt
     3481 ctggttttcc atcccgtttc cggtcgtccc ggagagcgac gttgcgcgga cggcggaccc
     3541 tgagttcctg caggaggctt gcgagtgtga agcgccgatg ggttcaaggg ggaaaatact
     3601 tctcgtggag gatactccgg tgaatcagca ggtcgccgtc gcgctcctcg ccaggatggg
     3661 gtacatggcc gacgtcgcgg ggaacggcga ggaagcgctg acgcttcttg actccatccc
     3721 ctacgatctg gtcctgatgg atattcagat gcccgtcatg gacggatttg agacgactgc
     3781 gctcatccgc tcccgagaag cgtccgaaaa gagagagaga attccgatcg tcgcgatgac
     3841 ggcgcacgcg ctctcaggat accgcgagaa gtgcatcgag gcgggaatgg acgactacgt
     3901 gagcaaaccc atattgccga aggagctgga gacagcgctc cggcggcatt tgaaaaaacg
     3961 atccgcggaa cgcgaagaag aaattgcagc agaggaggtt gcggaagagg tgaaggcgga
     4021 aaaaggaact gaaaacgtcc ttgagacggc agagagttcg cagtgggagg atatcccgct
     4081 cttcgatcag gcagacggcg ctgtgcggac tgccggggat gtcgtttttc tgggaaaact
     4141 ccttcttctc ttcgccgact cgaacgccgg ggaggaaggg cgtctccgcg agtttgccgg
     4201 acgcggggag tgggaatcag cgaagcacgc ggcgcactcg ctcaaaggtt cctccggaaa
     4261 cctgggcctt ctccgtctcg ccgaacaggc acgctggacg gaactgcggc ttgccgaata
     4321 tatggcgggg caatccgaac tggatgagaa cgccctgcgt tccgccgttc tctccctcgc
     4381 agatctcgcc tccgagacaa tggagctggc gcggcggcag ggaacaattc tgggcaagga
     4441 ggcggaagaa gccccctcct gatcctctac gcggccgggc tgaattgtgc tatccttttc
     4501 ggcgaggatt gaccgaatga tggagggatg aatcatgaca gcaccgcgcc aagagagtcg
     4561 agccgcagga gagccgaacg cctgccgcgc gatcagcggc agggaggcgc ttcgcgcgct
     4621 tgcctgcata gcgcatctta tccgggagat cttcccggga gacatgagtt tcgcggtcat
     4681 cgaggacgac gagtatctcg tctatgtgcc gggcaggacg atcgatatca agaagaaacc
     4741 cggtgacaag ctgaccgagg ggtcggcggg ccaccggtgc atgatggaaa aacggaagat
     4801 cgtcaaggag ttcaagaagg aggattcgcc gttcggcttc ccgtacgtcg cggcctccgt
     4861 gccgatcttc gacgcttccg gaaaggcggt cggctgcgtc atcacggcgg agaacatcac
     4921 ccgtcaggag acgatgcggg aaaccgcgga gctgctgcgc gcctccacgg ggcagatagc
     4981 ggactctctc cagacgatga ccggacagat ggaggagatg cgcgcggcgg gcgagatgct
     5041 gaactccatc acatcgcagg ccgtgagcaa ggtggaggga accgaacagg tgttcggcgt
     5101 catcgagggc gtggcgaagc agacgaatat gctcgggctg aacgcctcga tcgaagcctc
     5161 ccgcctggga gatcagggga aggggttcgg cgtcgtggcg gaggaggtgc ggaagctcgc
     5221 cgtccgttcg tccgactccg cacggcagat caaggagatc ctggagtact tcaagaactc
     5281 cacgatcgag atcaacagca gcaccgacac cctgctccac atcatcaagg aacagtcgag
     5341 aatcatcgag accatcgccg cgtccagcga ggagatggcc tcggtctctg cgatgctgga
     5401 gaagctctcc tcctcgctcg ggcaagggag ctgagacgtg tcccggcggg aaggacgatc
     5461 tccgtcagcc cgctcttttt gaaaaacatc gcaggatcgc gagacgttgc cttctcccga
     5521 gtcctgcgtt ttttgccaag gaggaatatg tagtggaaac tcttcgcacc cacgcgctct
     5581 tttttctctg cctcgccctt gcagtggcgc tggcgctgct tcctccgacc gcgccctacg
     5641 ccgtcggtat ggcgctcgta ctgggcgcgg cggtgagcaa cctcgccccc ggctcggcgc
     5701 ctcccggact ggggaagatg cgcaaacttg cgctgaacag cgccgtcgtc ctcttcgggt
     5761 tcggtctgaa catccgacag gtgatctccg tcggcggcca ggggttctgg cagaccgcgg
     5821 tcagccttgc ggtgatcatc tcgctcggtt tttttctgct ccggtttttc aaggtcgagg
     5881 cggcgacgct gaagctggtg accttcggca cgtccatctg cggcggaagc gccattgccg
     5941 ccgtgtcctc ggtgatgaag gttcgcgacg aggacatcgg cgtcgccatg ggcgtcgtct
     6001 ttctgctgaa cgccgtggcg ctcgtcgcct ttccgctgct tgcgtcgttg cttccgctca
     6061 cgccggagca gtacggcgtc tggtgcgcgt tgagcatcca cgacaccagc tcggttgtgg
     6121 gggcggcggc ggtgctggga gatgcgtcgc tgcagaccgc gaccatcatg aagctcaccc
     6181 ggacgctctg gatcacgccc atcgtcttcc tgatctcgct gcggcagggc gagaagggca
     6241 agctctccat cccgctcttc atcgtcttct ttctgctggc ctcggtgacg gcgtccgtcc
     6301 ttcccttcgc cccgttcttc aagacgctcg cttcgtccgg gaaggtactg atggcggttg
     6361 cgctctatat cgtcggcttc ggcctgcacc gcagcgtcct caagaaggtc ggcgcgcagg
     6421 ggatgctctt cggtatgctg ctctgggcgg tctctatcgt tatggggtac ctcctcgcat
     6481 caatgagctg acattcgaca caaaggagat ggaataagat gaatctcgca cgctttccca
     6541 gaagacgcta taccgagggg cggacccctt tggaatttct cgaacggctt tcagcaaagc
     6601 tcggcggccc ggagatatgg atcaagcggg acgacatgct cggctttctc ccgggaggga
     6661 acaagacccg gaagctcgaa ttccttatgg ccgacgcgtt ggcgcagggc gcagacgccg
     6721 tgatcacctg cggcgcggtg cagtcgaacc actgccgtct caccctcgcc gccgcgagga
     6781 aggaagggct cgagtgccac ctcgtcatag aggagcgcgt tccggggagc tactccgaac
     6841 aggcttccgg caacaacttt ctctttcacc tgatgggagt ggactcgatc cgcgtcgttc
     6901 cggccaaatc ggacatgctc gctgagatgg aaaagaccgc ggacgagctg cgccgcgcgg
     6961 gaaaaaagcc ctacgtcatt cccggcggtg gttccaacgc gctcggcgcg ctcggctacg
     7021 tcgcctgcgc cgaggagatc ctggctcagg cgttcgacaa agggg
//
LOCUS       DAOSWW010000022         7027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_6971_length_7027_cov_4.286575, whole genome shotgun sequence.
ACCESSION   DAOSWW010000022 DAOSWW010000000
VERSION     DAOSWW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6971_length_7027_cov_4.286575"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /gene="plsX"
                     /locus_tag="PK442_00925"
     CDS             complement(<1..828)
                     /gene="plsX"
                     /locus_tag="PK442_00925"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOO86237.1"
                     /translation="MILALDAMGGDHGPSEICKAALLACEEYSDLELLLVGSEPLIKP
                     LLEKAPSSLAKRLSIIHTDERIDMDEHPTLAIRKKRNSSMRLAMEMVRSGEASGCVSS
                     GNTGAIVAGGVLVVGRISGIDRPAMGVALPTLNKYTFLLDVGANVRNKPYHLFQFAHM
                     GNIYSKRILGVTTPQIALLSNGSEDIKGDEVISGAKTLIKESALNFSGYVEGDDIPYS
                     RADVVVCDGFSGNIVLKFAEGLMTAVASMLKEEIGHRILPKVGMVFMLPMMKDLIARF
                     "
     gene            complement(852..1421)
                     /gene="fapR"
                     /locus_tag="PK442_00930"
     CDS             complement(852..1421)
                     /gene="fapR"
                     /locus_tag="PK442_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HOO86238.1"
                     /translation="MKSQRKKNRQEKLLKLIEQNPLATDEQLAGILSASISTIRLDRA
                     VLAVPELRERMRSMAQKATSRLRSLKQSEVVGDLLELEPNKWALSVLETRREMAFRET
                     DMVWDHYIYAQASSIAIAVVEADFVIVDSMRGEYKGHARVGDALIARAKVGVRKDDKY
                     IVSVRTKVGDREIFVGRFIAAIVAENRLF"
     gene            complement(1595..1792)
                     /gene="rpmF"
                     /locus_tag="PK442_00935"
     CDS             complement(1595..1792)
                     /gene="rpmF"
                     /locus_tag="PK442_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOO86239.1"
                     /translation="MATPKSRVSHARTHKRKSEWIRSLSGPELTTCPHCGEVVMNYTA
                     CGECGFYRGRKVLETKTEAKE"
     gene            complement(1811..2431)
                     /locus_tag="PK442_00940"
     CDS             complement(1811..2431)
                     /locus_tag="PK442_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOO86240.1"
                     /translation="MPITEKPDSWRFEIDTPELGNEDAVTTALWEQEVLSRPGLGSAP
                     DSARAEILYDGLLFRFASPLKIEAEARWADDSLCVTIAVKAFFTTQCSRCLEPTDIEI
                     LDDFMYLYSLRKKTAANRNATEETDDEYQVVPVASWKNRLDISDQVWESLILTLPMQV
                     LCSRDCRGICPTCGKSLNSGECICSSETKDPRFEKLLAIQFDQDTE"
     gene            complement(2459..3658)
                     /locus_tag="PK442_00945"
     CDS             complement(2459..3658)
                     /locus_tag="PK442_00945"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOO86241.1"
                     /translation="MKILVVNCGSSSLKYQLFDMTDESVLAKGLIERIGIEGARIKHT
                     KTGMDAVIKNREIPNHKVAFKVVVEALLDPEHGVLKSLDELAAVGHRVVHAGERYAGS
                     VLITHDVIDALEECVPLAPLHNPANLMGIYAVTEALPNIPQVGVFDTAFHQTMPGYAY
                     MYGVPYHYYEKYKVRRYGFHGTSHYYVSHRAAEILGKPVESLNIVTCHMGNGSSLAAV
                     KGGKSVDTSMGFSPLPGVIMGTRSGDVDPLLISYLSDQEGIDNKAMSHVLNKESGLLG
                     ISGVSSDLRDIEEAADSGNQRAKLAEDMLYYGVKKYVASYAAAMGGIDVLVFTAGIGE
                     NSARARESICAGLEFMGIKLDKEKNNVRGKERIVSTDDSRVKVIVIPTDEELVIARDT
                     KKIAIKN"
     gene            3758..4990
                     /locus_tag="PK442_00950"
     CDS             3758..4990
                     /locus_tag="PK442_00950"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO86242.1"
                     /translation="MVAEYNPLHNGHQHHIRRTREKVNPDGIVVVLSSYFTQRGEPAL
                     LSKWDRAESALHAGANLVLELPVFFSSHNAGVFASGAVDVLAATGLVQTISFGMEEPD
                     FDVAPLVDILVQEPITFKDSLKKHLNSGFSYVKARAEALASINAEYGVFVSSPNNSLA
                     LAYMEHIARKGLNIQCMPIQRTGAGYHNTSVTASGEESCFASASAIRRELLESGLDAV
                     AHTLPVPSFEILRRSMETGYYVLSTDLLWRMIRMVLLRTTKKNLSHSSEMTEGMENRL
                     LRYASECGSWDEFLRKCVTHRYPRARIQRQLIHLLLGIDHTENRTFQENGPAYIHPLA
                     ADKKGVEMLRAMRKKASLPIRGKLPLHAQGAEGKLASIECLAAEIWESLTPAFIPGTE
                     KKRHLIRGQEYAEEGNAP"
     gene            complement(4950..5456)
                     /gene="coaD"
                     /locus_tag="PK442_00955"
     CDS             complement(4950..5456)
                     /gene="coaD"
                     /locus_tag="PK442_00955"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOO86243.1"
                     /translation="MKRCISAVYPGSFDPITNGHIYIAERAAALFDEVIVSVLLNPQK
                     KAAFTVDERKAMAREALSHLTNVKVGSFSGLLVDFLRQERSRIIIRGLRALSDFEYEF
                     QLALMNRQLAPDIETLFIVTDARYSYLSSHAVKEIYQFGGAVHEMVPPGVFRRLRERF
                     PLPHTLAP"
     gene            complement(5453..6013)
                     /locus_tag="PK442_00960"
     CDS             complement(5453..6013)
                     /locus_tag="PK442_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOO86244.1"
                     /translation="MTRGKKGAPPKEVRPTSGKVLGALFSILGPLQGRSFLDLFSGTG
                     RVAYRAAQQGASFVAAVEILPVRARDIRSLFPAAPSFLLLAMDIRRALSFLERKGHRF
                     EVIFADPPYGAGWPGILGELLFPKSGGILVPNGVAVIEHSLREPIPSGENYRIIDRRE
                     YGETVLSFLASAVLPTDTSGERGEKV"
     gene            complement(6010..6990)
                     /gene="trmB"
                     /locus_tag="PK442_00965"
     CDS             complement(6010..6990)
                     /gene="trmB"
                     /locus_tag="PK442_00965"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOO86245.1"
                     /translation="MPEKQQLPLNILRARGETRVFLEIGFGNGEFLVHLARQNPDAVF
                     WGVEMSRSCVLRALKRIRREELRNIFLLCGDARFILKECLPKESLDGIYMNFPCPWPK
                     KKHTKRRVSSGDFPSEIASVLKKGAFFELVTDEEWYGMEVRNVLAAHPLLELTEWKIN
                     PPRPVTTKYERKWMEMGKRIFLSRLQKKEAESSVGYRCLGRTEDVHIRVPGKGSLKKF
                     VNDIHNMGGEKGDALWVYKRYYESAEGNAYIIETVAGDGSFEQKFFIQIVEREEDALI
                     KIAPYSSPFLTDSVRGALEGMAHLIETHYLSIASSCAASLEQPAGKAFSQ"
BASE COUNT         1680 a         1848 c         1725 g         1774 t
ORIGIN      
        1 aaagcgtgca atgagatctt tcatcatagg gagcataaag accattccca ccttgggaag
       61 aatccgatgg ccgatctctt ctttgagcat agaagcgact gcagtcatca ggccctctgc
      121 gaatttgaga acgatgtttc ccgagaagcc gtcgcagaca accacgtccg ccctgctgta
      181 cggaatgtcg tctccctcaa cgtatcccga aaaattgagc gcgctctctt taataagcgt
      241 ttttgcgccg gagatcacct cgtcgccctt tatatcctcc gagccgtttg agagcaacgc
      301 tatctgcggc gtcgtaacgc ccaggatgcg ctttgaatag atattaccca tgtgagcgaa
      361 ctgaaaaaga tgatacggtt tattccgtac gtttgcgccc acgtcgagaa gaaaggtgta
      421 cttgttcagc gtggggagcg caacacccat tgcaggacgg tcgatcccgg aaatcctgcc
      481 tacgacaagc actcctccgg caacaatcgc acctgtgtta ccggaggata cacatccgga
      541 tgcttcgccg gaacgaacca tctccatcgc aagacgcatg ctggagtttc ttttttttcg
      601 tatggcaagc gttggatgtt cgtccatatc tatacgttca tcagtgtgta taatggaaag
      661 acgctttgcc aaggaagaag gagctttttc cagcagaggc ttgatgagcg gttccgatcc
      721 aacgagcaga agctcaagat cgctatactc ttcgcaggcg agcagcgccg ccttgcatat
      781 ttcggaaggg ccatggtcgc ctcccattgc gtcaagggca agaatcaaac agggttcacc
      841 tctctccaga ctcaaaaaag cctattttcc gcaacaatag cggctataaa ccttccgaca
      901 aaaatctctc tgtctccgac cttcgtccgt acgctgacta tatatttatc atccttgcgg
      961 actcctacct tcgctctggc tataagggca tctcccacgc gagcgtgtcc tttgtattcg
     1021 cctctcatcg agtccactat tacgaaatca gcttctacca ccgcaatcgc aatagaactt
     1081 gcttgagcat agatgtagtg gtcccatacc atgtccgttt cgcggaacgc catttccctg
     1141 cgggtttcca aaacggacag agcccatttg ttgggttcca gttcaagaag gtcgccgaca
     1201 acctcgcttt gctttaaaga acgcaatcga ctggtggctt tctgcgccat agaacgcatt
     1261 ctttcgcgaa gctccggcac ggcaagaacg gccctgtcaa gacgaatagt actgatactc
     1321 gcggaaagaa tgcctgcgag ttgttcgtct gtcgcgagcg ggttttgttc gatgagtttc
     1381 agcaacttct cctgtcggtt tttttttctt tgagacttga tggaagtcgc ccccctcaat
     1441 aaccacctga tgataacatc tgctctcaag acaaggtaag tataattggg ttacagcgag
     1501 cggtcaatag gtgaagcatg gaagatgaaa caaaaagaga gagggtttcc cctctctctt
     1561 ttgcaactaa accgttctga gtatagtctg caagctactc ttttgcttcc gtcttggttt
     1621 cgagtacttt tcggcctcta taaaatccgc attctccgca agccgtgtag ttcattacca
     1681 cttctccgca gtgcggacat gtggtcagct cagggccgga caaggaccgg atccactccg
     1741 actttctttt atgtgtacgg gcgtgggata ccctgctttt cggcgtcgcc attctgcatt
     1801 cccctccttt tcactctgta tcctgatcga attgtattgc aagaagcttt tcaaaccgcg
     1861 ggtcctttgt ttcggaagag caaatacatt ctcccgagtt cagagacttt ccgcacgtcg
     1921 gacatatgcc gcggcaatcc ctagagcaga gcacctgcat cggaagagta agaatcaagc
     1981 tttcccagac ctggtccgaa atgtccagac gatttttcca ggaagcgact ggtactactt
     2041 gatactcatc atccgtttcc tccgtagcgt tacggtttgc ggcagtcttc ttccgcaaag
     2101 aataaagata catgaaatca tccagaattt caatatctgt cggctcaaga catcgagaac
     2161 attgcgttgt aaaaaaagct tttacggcaa ttgtaacgca caaactatcg tctgcccaac
     2221 gcgcttccgc ttctatcttc agcggggaag caaaacggaa aagaagcccg tcgtaaagaa
     2281 tttccgcccg ggctgaatcg ggtgcggaac caagtccggg ccgggacaga acttcctgct
     2341 cccaaagggc cgtggtaacc gcatcctcat tcccaagctc cggggtatca atttcaaaac
     2401 gccatgagtc cggtttttcc gttattggca cgtccttccc ctcctagggc tctagcgact
     2461 agtttttaat cgcgatcttc ttagtatccc gagctataac gagctcttcg tccgtcggta
     2521 ttacgattac ttttacacgg ctgtcgtcgg tgctgacaat gcgttcttta ccacggacgt
     2581 tatttttctc tttgtccaat ttgattccca taaattcaag gccggcgcag atgctctcgc
     2641 gggcgcgggc gctgttttcc cctatgccgg cggtgaagac aagcacatca atgcctccca
     2701 tggcggcagc atacgacgca acgtatttct tgacgccgta atagagcatg tcctcggcaa
     2761 gtttggcccg ctggtttccg gaatcggccg cttcttctat atcacggaga tcgctgctga
     2821 cgccggaaat accaagaagc ccgctttcct tgttcaggac gtgactcatc gccttgttgt
     2881 cgattccttc ctggtctgaa aggtaagaga tgagcagcgg gtcaacgtcg ccggatcgag
     2941 tccccataat gacgcctgga agggggctga atcccatgct tgtatcgacc gacttcccgc
     3001 cttttaccgc ggcaagagag ctgccgttcc ccatgtggca tgtgacaata ttgagcgact
     3061 cgacaggttt gccaagaatt tcagcggctc tgtgggaaac atagtagtgg ctggtaccgt
     3121 gaaagccgta gcgtcgcact ttgtacttct cgtagtaatg gtaggggacg ccgtacatat
     3181 aggcatatcc gggcatggtc tggtggaacg ccgtgtcgaa tacgcctacc tgggggatat
     3241 ttggaagggc ttctgtaaca gcgtaaatgc ccatgaggtt ggcggggtta tgaagaggag
     3301 caagagggac gcactcttcc agagcatcga tgacgtcatg ggtgataagg accgagcctg
     3361 cgtaccgctc tccggcgtga acaaccctgt gcccgacggc ggcgagttca tcgaggctct
     3421 tcaatacgcc atgttccggg tcaaggaggg cctcgacgac gactttgaag gcaactttgt
     3481 gattggggat ttctctgttt ttgattacgg cgtccattcc ggtcttcgtg tgcttgattc
     3541 gggctccttc aatgcctatt ctctcaataa gcccctttgc cagcacagat tcgtcggtca
     3601 tatcgaaaag ctgatacttg agagatgaac taccgcagtt tacaacaaga atcttcatga
     3661 atactacccc ttcctctcat cttgaccgga cgctttcgca ggttgtattg taataaacaa
     3721 aaggaggccg gtcgccatat atggaaatta tgttggtatt gtagccgagt ataatccatt
     3781 gcataacggc catcagcatc acatacgccg aacgcgggaa aaagtaaatc ccgacggcat
     3841 tgttgttgtg ctctcatcct atttcacaca aagaggagag cccgcgcttc taagtaagtg
     3901 ggatcgtgcg gaaagcgcgc ttcatgccgg agctaacctt gttctggaac tgcctgtttt
     3961 cttttccagt cataatgcag gcgtctttgc atcgggagcg gtcgatgttc ttgcagccac
     4021 cggcctggtt caaaccattt ctttcgggat ggaagaacct gattttgatg ttgctccact
     4081 ggttgatatt ctagtccagg aaccgattac tttcaaggat tctctgaaaa aacatctgaa
     4141 ttcgggattt tcctatgtaa aagcgcgagc ggaagctctt gcttcaatca atgctgaata
     4201 tggcgttttc gtctcttccc ctaataattc actcgcattg gcgtacatgg agcatattgc
     4261 gagaaaaggg ctgaacattc agtgcatgcc aattcagcgg accggcgccg gttatcacaa
     4321 tacttccgtt accgcatccg gcgaagaatc ctgttttgcc agcgcctccg ctatacgtcg
     4381 cgaactactt gaaagtggac tcgatgctgt ggcgcacacg cttccggtcc caagctttga
     4441 aattctgcgg cggtctatgg aaaccgggta ttacgttctc tcgaccgatt tgctctggag
     4501 aatgatacgt atggttctgc tgcgcacaac gaagaaaaat ttgagccatt catcggagat
     4561 gacggaaggg atggaaaaca ggttgcttcg ttatgcctca gaatgcggct catgggatga
     4621 atttttgagg aaatgcgtga ctcatcgata cccgcgagcc agaatccaac ggcagctcat
     4681 tcatcttctg ctgggtattg atcacaccga aaaccggact tttcaggaaa acggtcctgc
     4741 gtatattcac ccgcttgcag cggataaaaa gggagtggag atgcttcggg caatgcggaa
     4801 gaaagcatcg cttcccatac gagggaagct gccccttcac gcgcagggag ccgaaggaaa
     4861 gttggcttct attgaatgtt tggccgcaga aatctgggaa agtctcaccc ctgccttcat
     4921 tcccgggact gaaaaaaaaa ggcatctcat cagggggcaa gagtatgcgg aagagggaaa
     4981 cgctccctga gacgccggaa gacccccggc ggaaccattt cgtggacggc tcctccgaac
     5041 tgatatatct cctttacagc atggcttgaa agatatgagt accttgcgtc ggttacgatg
     5101 aacaacgttt ctatatcggg ggctaattgt ctgttcatca gagccagctg aaactcgtac
     5161 tcgaaatccg agagcgcccg gagccccctg atgatgatac ggctccgttc ctgtcgcaga
     5221 aaatcaacga gaagaccgga gaaggaaccg actttgacgt tggtcagatg gctcaacgcc
     5281 tctctggcca tggctttcct ttcgtcgacg gtaaacgccg cctttttctg aggattcaag
     5341 agaacgctta caatgacttc atcgaagagc gccgctgctc tctccgcgat gtagatgtgt
     5401 ccgttggtga taggatcgaa cgacccgggg tagactgcgc ttatacatct cttcatactt
     5461 tttctcctct ctctccggaa gtatctgtgg gcaaaacggc cgatgcaaga aaagaaagga
     5521 ccgtctctcc gtactcgcgg cgatcgatga tcctgtagtt ttcgccggaa gggatgggct
     5581 cccgaaggga atgctctatt accgcaactc cgttcggaac gagaatacct cccgatttcg
     5641 gaaaaagcag ttccccgagg atgccgggcc accccgcacc atacggcggg tcggcaaaga
     5701 ttacttcaaa acgatgacct ttccgttcca ggaaagaaag cgctcttcga atatccatcg
     5761 cgaggagaag aaaagagggg gcggcgggga atagagaacg aatatcccgg gcacgaacgg
     5821 gcaatatctc caccgctgca acaaaagaag ctccctgttg ggctgcgcga tacgcaaccc
     5881 tgccggttcc ggagaaaagg tcgaggaaag agcgaccttg caagggaccg aggatactaa
     5941 agagcgcccc tagaaccttt ccggaagtag ggcggacctc cttgggcggc gcccccttct
     6001 ttcctctggt cactgcgaaa acgcctttcc agcaggttgc tcaagtgaag cggcgcagga
     6061 ggaggcgatc gatagatagt gcgtctcaat caagtgagcc ataccttcga gagccccgcg
     6121 tactgaatca gtcaaaaacg ggctcgaata cggcgctatt tttatgaggg cgtcctcttc
     6181 gcgctctacg atctggatga aaaatttttg ttcgaaggag ccgtcgccgg ccactgtttc
     6241 gataatataa gcgtttcctt ccgcagattc gtaatacctt ttatacaccc agagagcgtc
     6301 gcccttttct cctcccatgt tatgtatatc gttcacgaat ttttttaaag atccctttcc
     6361 cggaactcga atatgcacgt cctccgtcct ccctagacag cggtatccga cagatgattc
     6421 cgcttctttt ttctgcagtc tggaaagaaa aatacgtttc cccatctcca tccatttgcg
     6481 ttcgtatttt gttgtaaccg gcctgggcgg atttatcttc cactccgtaa gttcaagcaa
     6541 aggatgagcg gccagcacat ttcgcacttc cataccgtac cattcctcgt cggttacaag
     6601 ttcaaagaac gcccccttct tgagaacgct cgcaatctcc gagggaaaat ctccgctgga
     6661 aactcttctt ttagtgtgtt ttttcttagg ccaaggacag ggaaaattca tgtaaatgcc
     6721 gtcgagagat tcttttggaa ggcattcttt cagaatgaaa cgcgcatcgc cgcacaggag
     6781 gaagatgttt cgcagttctt ctctgcgaat tcttttcaga gcgcgcagaa cgcacgaccg
     6841 ggacatctcg acgccccaaa aaacggcatc cgggttttgc cgtgcaagat gaacgagaaa
     6901 ttcaccgttt ccgaaaccta tttcaagaaa tacgcgcgtt tcccctctcg ctctcagtat
     6961 attcagagga agctgttgct tttcaggcat gataataatt tctcgtatgt cgatcacaat
     7021 tccaacc
//
LOCUS       DAOSWW010000023         6815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_7266_length_6815_cov_4.106805, whole genome shotgun sequence.
ACCESSION   DAOSWW010000023 DAOSWW010000000
VERSION     DAOSWW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7266_length_6815_cov_4.106805"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PK442_00970"
     CDS             <1..1100
                     /locus_tag="PK442_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOO86246.1"
                     /translation="GALCAAGVVFMTLVGLNRALRTTGFWTASRPPPDLLQDLDLVAL
                     ATIADCMPLTPLNRALVRQGMDILSTTPRKGLARLFSRLGIARPGSALTEETLSMKVI
                     PCINAAGRMDFADTAVNVLLGSPNVDESVEMLISLNRKRQSLSLKISKEAELVLSGGV
                     NVILGESWPVGVLSGVASRICGATGAPVALAAPVNGGIRGTLRVPEGADAVGVLSALS
                     SKLEAWGGHKYAAGFSVSRENWDFVRRDLQQALSSMKVEETSVTAIAMAPEEISIESW
                     KEACTLGPFGNGNPPPMFFLARKGRERMLPLGKDGKHIQIDAGGIRLLAFHGENEREA
                     LAGADGWVYRPRIDCWRGEERLQYVVDYAVV"
     gene            1160..3535
                     /locus_tag="PK442_00975"
     CDS             1160..3535
                     /locus_tag="PK442_00975"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOO86247.1"
                     /translation="MDPSKLTESILESRTHPVRTGALPDQDPAAESGEVSSLRSGGTA
                     DLDRKSSGKLRDSYIGRIPEEERAISVKFAWHELWSKVIVYLSREELMQLGEALVMAG
                     AAHKEQRRSTGDPYIVHTINVASILADMQLDNVTLMAALLHDVLEDTDITMEKLTGAF
                     GAPVAMLVDGVTKLGKLPFKSFEDYQAENLRKMFVVMAKDIRVVLIKLADRLHNMRTL
                     GALRKDKQQRIARETLEIYAPLAHRLGIYQVKRGLEDLAFKYADPEMYYEIRRRVRKK
                     LPERETVVKKALEVLTARLEEEGIRCKVKGRAKHFYSIYEKMNRKKLPVEQLYDLLAL
                     RVMVDDIAACYTVLGIVHTIWKPIPGQFDDYIANPKSNMYQSLHTTVVGPTGEPLEVQ
                     IRTWDMNSLAEYGIAAHWRYKEGGASLDDLDAKLTWIRQAIEADHDGSEPSEFLERLK
                     EDVLSSDVFVFTPQGEVVSLPKGSTPLDFAFAIHTQVGTRCVGAMVNNRIVAMGYELQ
                     NGDIVKIITSPQGAPSRDWLKIARSSKARSKIRAYFRALEKTERLEKMQRGRDLLERE
                     VKRRGTPEFEEFDELLPLLNKIARDTAYSNGEELLSAIGGGHQSVSLVAQRLAGKPQP
                     QPAPVQSLPPEPEKHTSFSGKKGDSDIVVEGADGVQVVLSNCCEPVPGDPIIGFSTRT
                     RGITVHRADCENVRNSREERRVSVSWGMTGTRRFSARLKIEAVDRAGLFGDVAQAITA
                     GDGSMTGIKAGVVGGNQARMKLEVRVRDLEHLYLIVARLNAVKGMIQVSRG"
     gene            3543..3980
                     /gene="dtd"
                     /locus_tag="PK442_00980"
     CDS             3543..3980
                     /gene="dtd"
                     /locus_tag="PK442_00980"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOO86248.1"
                     /translation="MRAVVQRVSSARVSVDGETKGAIGKGVCVLLGVSDADGEQEADW
                     MAEKIVNLRIFEDDEGKMNRSLLEVGGSMLAVSQFTLYGDCRKGRRPSFVDAGEPGHA
                     NRMYESFKSAAAGRGVEVESGVFQAHMLVEIANDGPVTLIIDR"
     gene            4089..4739
                     /locus_tag="PK442_00985"
     CDS             4089..4739
                     /locus_tag="PK442_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO86249.1"
                     /translation="MKYTRFPLGPLWTNSYLVWDASGDGFLVDPAGDAAEILSFAREN
                     NIVVRAVLLTHGHLDHVGGLPELLEKIASPVYIGEGDAEMLRSPDQNAAEWMGYDFKG
                     IRNFFTLRDGEQLPVGAMSVSVLETPGHTPGCVCYHVSESSDTGEETLLLSGDTLFAR
                     SVGRTDLPGGNGERLHESLIRLAALPDTLRVLPGHGPETTIGAERIHNPFWPEDKA"
     gene            4736..5407
                     /gene="radC"
                     /locus_tag="PK442_00990"
     CDS             4736..5407
                     /gene="radC"
                     /locus_tag="PK442_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOO86250.1"
                     /translation="MKLASLPREEMPRERLRLRGASSLSLPELLAILLRTGSRGKDVL
                     ELAADVLNEFGGAKGMARATEEEMLGFPGLGDAKACVLLAALELGKRLEAARRELQEG
                     KDWRRSVDEIAGELAFEEREYIFAIHVRSNGSVIHVDRISFGGAEGAYLDVKYLLRRA
                     VRMDSYGLVLVHNHPDGSLQPSREDRLLTDFVSRRTKILDIHLLGHFVVANGESHGIA
                     LPGEP"
     gene            5427..6482
                     /locus_tag="PK442_00995"
     CDS             5427..6482
                     /locus_tag="PK442_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOO86251.1"
                     /translation="MFKFLTGVFGGDVGIDLGTANIVVYVKGRGIVISEPSAVAIRKR
                     PRGGATEVIAVGREAKAMAGKTPGGVFTIWPLQDGVIANFEMTEELIRHCLRRANGGK
                     PFVSHPRVVISVPAEVTEVERKAVIDATLGAGAKEAYVIEEPVAAALGVGLPIQEPRG
                     SMILDIGGGTSEVGVLSLGGIVVTNSLRAAGKEMDNAIVAMLRQRYALLIGDTTAEEV
                     KIAIGSALPLDKELEMAVKGRDLADGLPKADVVTSVEVREALEPILLRIEDMVKVALE
                     QTPPELAKDIVDQGIFLSGGVSLLRGLAERLSKAMNTPVVVAEDPLFAVARGVGKILE
                     NLGDMKKVLLSVEKGSK"
     gene            6510..>6815
                     /locus_tag="PK442_01000"
     CDS             6510..>6815
                     /locus_tag="PK442_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86252.1"
                     /translation="MTEKYSEREWIHGLLALAASLLLLAAAPRREFFRGAVDFVGEVL
                     RYPEYPAVFIERTTREFSVWFADRYVLQERLNTLKEENERLNLVWSMENARNLRRELD
                     "
BASE COUNT         1373 a         1919 c         2257 g         1266 t
ORIGIN      
        1 tcggcgcgct ttgcgcggcc ggcgtggtgt tcatgacgct ggtcggcctc aatcgcgctt
       61 tgcgcacgac gggcttctgg accgcgtcgc gcccgccgcc ggacctgctg caggacctgg
      121 atctcgtcgc gctcgccacg atagcggact gcatgccgtt gacgccgctc aacagggcgc
      181 ttgtccggca gggtatggat attctctcga caaccccccg caaggggctc gcacggcttt
      241 tttcgagact cgggatcgcc cgccccggaa gcgcgctgac ggaggagacc ctctcgatga
      301 aggtgatccc ctgcatcaac gccgccggga ggatggattt cgccgatact gcggtgaacg
      361 tgctcctcgg ttctccgaac gtggatgaat ccgtggagat gctgatttcg ctgaatcgga
      421 agcgccagtc gctttcgctg aagatatcga aagaggcgga gctggtgctc tccggaggcg
      481 tgaacgtcat actcggggag tcctggccgg tcggggtgct cagcggcgtc gccagcagga
      541 tttgcggcgc cacgggagct ccggtggcgc ttgcggcccc tgtgaacggc ggcatacgag
      601 gaacgctccg cgttcctgaa ggcgccgatg ccgtcggcgt gctctccgcc ctctcttcaa
      661 aattggaggc gtggggaggg cacaagtacg ccgccggttt ttccgtctcc agggagaact
      721 gggattttgt ccggcgcgat ctccagcagg cgctttcctc gatgaaggtc gaggagacgt
      781 ctgtgacggc gattgcgatg gcgccggagg agatatccat agagtcctgg aaggaagcgt
      841 gcacgctcgg tccattcggc aacggcaacc cgcctccaat gttttttctc gcccgcaagg
      901 gaagggagcg aatgctcccg ctcggcaagg acggcaaaca tatccagatt gacgcgggcg
      961 ggattcgcct tctcgctttt cacggggaga acgagcggga ggcgctcgca ggagcggacg
     1021 gatgggtgta ccgcccacgg atcgactgct ggcgaggcga agagcgtctg cagtacgttg
     1081 tcgactacgc cgttgtttag caaggcgcac cccggagttc tttggaagcg atcggttcca
     1141 gatgtgagcg gagggaaaaa tggaccccag taaactgacc gagagtattc tggaaagccg
     1201 gacgcatccc gttcgtacgg gagcgttgcc ggaccaggac cccgcagcgg agagcggcga
     1261 ggtttcttcg ttgcgctccg ggggaacggc ggatcttgac aggaagagct cgggaaagct
     1321 ccgcgacagc tatatcggcc gtattccgga ggaagagagg gcaatatcgg tcaagttcgc
     1381 atggcacgag ctctggtcga aggtgatcgt ctatctctcc agggaagagc tgatgcagct
     1441 cggcgaggcg ctcgtaatgg cgggcgccgc ccacaaagag cagcgccgct cgaccggcga
     1501 tccgtacatt gtccacacga tcaacgtcgc ttccattctt gcggatatgc agctcgacaa
     1561 cgtcacgctg atggccgccc tgcttcacga cgttctggag gataccgaca tcaccatgga
     1621 gaaactgacc ggcgctttcg gcgctcctgt ggcgatgctg gtcgacgggg tgacaaagct
     1681 cggcaagctc cccttcaagt cgttcgagga ttaccaggcg gaaaacctgc ggaagatgtt
     1741 tgtcgtgatg gccaaggata tccgcgtggt gctcatcaag ctcgcggatc gtctccacaa
     1801 catgcgcact ctgggagccc tcaggaagga caagcagcag cgcatcgcgc gcgagacgct
     1861 cgaaatctat gcgccgcttg cgcaccgcct cggtatttat caggtgaaac gcggacttga
     1921 ggatctcgca ttcaagtacg ccgatcccga gatgtactac gaaatcagac gccgcgtccg
     1981 gaaaaaactc cccgaacggg agaccgtggt caagaaggcg ctcgaagtgt tgaccgcccg
     2041 gcttgaagag gaagggatcc gctgcaaggt aaaggggcgc gcgaagcact tttacagtat
     2101 ctacgaaaag atgaacagga agaagctccc cgtggagcag ctctacgacc tgcttgcgct
     2161 ccgggtgatg gtggacgata tcgccgcatg ctatacggtg ctcggcatcg tccacacgat
     2221 atggaagccg attccggggc agttcgacga ctatatcgcc aatccgaagt cgaacatgta
     2281 ccagtcgctc cacaccaccg tggtgggacc gacgggagaa ccccttgaag tgcagatccg
     2341 cacctgggat atgaactccc tggccgagta cggaatagca gcgcactggc gatacaaaga
     2401 gggaggcgcc tccctcgacg atctcgacgc gaagctgacc tggatccgtc aggcgatcga
     2461 ggcggatcat gacggctcgg aaccgtccga attcctggag cgcctgaagg aggacgtgct
     2521 ttcttcggat gtcttcgtct tcacgcctca gggtgaggtc gtttcgcttc cgaaggggtc
     2581 gacgccgctc gacttcgcct ttgccatcca tacccaggtg gggacgcgat gcgtcggcgc
     2641 gatggtgaac aaccgtatcg tcgccatggg gtacgagctg cagaacggcg atatcgtcaa
     2701 gatcatcacg tcgccgcagg gagccccttc gagagactgg ctcaagatcg cccgcagcag
     2761 caaggccagg agcaagatac gcgcctactt ccgcgccctc gaaaagaccg agcgcctgga
     2821 gaagatgcag cgcggccggg atctgctcga acgcgaagtc aagaggagag ggactcccga
     2881 gttcgaagag ttcgacgaac tgctcccgct gctcaacaag attgcccgcg acacggccta
     2941 ttcaaacggg gaagagctgc tctccgcaat aggcggcggc catcaaagcg tctcgctggt
     3001 ggcgcagcgc ctcgccggaa agccgcagcc gcagcctgct ccggtgcaat cccttccccc
     3061 cgaaccggag aagcatacct cgttttccgg gaagaagggg gattccgata tcgtggttga
     3121 gggggcggac ggggtccagg tcgtcctttc caactgctgc gagccggttc ccggagaccc
     3181 gatcatcggc ttttccacgc gaaccagggg gatcaccgtg cacagggccg actgtgaaaa
     3241 cgtcaggaac tcccgcgaag aacgccgcgt cagcgtgtcg tggggaatga cgggtacccg
     3301 gaggttctcc gcgcgcctga aaatagaagc tgtggacagg gccgggctct tcggcgacgt
     3361 cgcccaggca attaccgcgg gggacggcag tatgacgggc atcaaggcgg gagtcgtagg
     3421 aggaaatcag gcgcgaatga agcttgaagt tcgtgtccgc gaccttgaac acttatatct
     3481 tatagttgcc aggctcaatg ccgtaaaggg catgatccag gtatcgcggg ggtgacgctg
     3541 tcgtgagggc tgtggtgcaa agagtctcct ccgcacgggt ttctgtggac ggagaaacaa
     3601 aaggagccat cggaaaggga gtgtgcgtcc tgctcggcgt ttccgacgcc gacggcgaac
     3661 aggaggcgga ctggatggcc gagaagattg tcaatctccg tatcttcgag gatgacgagg
     3721 ggaagatgaa caggtcgctg cttgaagtcg gcggcagcat gcttgcggtc tcgcagttca
     3781 cgctctacgg agactgccgg aagggaagac ggccttcgtt tgtcgatgcg ggcgagcccg
     3841 gtcacgcgaa tcgaatgtac gaatcgttca agagcgcggc ggccgggagg ggcgttgagg
     3901 tggagagcgg cgttttccag gcgcacatgc tcgtagagat tgcgaacgac ggtccggtga
     3961 cgcttatcat cgaccgctga gtcatgtgcg gagggcgaag gcgcgcagtg cgcccccctt
     4021 cgcgaaaaga acctgcggcc gcatagcgcg gcgccgggaa gtatgcgtac aaaaggaagg
     4081 gatttatgat gaagtacaca cgatttcccc tggggcctct ctggacgaac tcgtatctcg
     4141 tatgggacgc ttcgggagac ggctttctgg ttgatccggc cggcgatgcg gcggaaatcc
     4201 tctcgtttgc acgggagaac aacatcgtcg tccgcgcggt tctcctcacc cacggacatc
     4261 ttgaccacgt cggcgggctg cccgaactcc ttgagaaaat tgcttccccg gtgtacatcg
     4321 gagaggggga cgcggagatg cttcgctcac ccgatcagaa cgcggcggaa tggatggggt
     4381 atgatttcaa ggggatcagg aactttttta cgctgaggga cggtgaacag cttcccgtcg
     4441 gcgccatgtc ggtctccgtg ctggagaccc cggggcatac gcccggatgc gtctgctatc
     4501 atgtatcgga gtcctcggat acgggagagg agaccctcct tctctccgga gacacgctct
     4561 tcgcccgcag cgtcgggcgt acggaccttc ccggaggaaa cggggaaaga ctgcatgaat
     4621 cgctgattcg cctggccgcg cttccggata cactccgggt gctgcccggc cacggtcccg
     4681 aaaccactat cggagcggaa cgcatccaca acccgttctg gccggaggac aaggcgtgaa
     4741 actggcctcg ctgccccgcg aggagatgcc gcgggagcgg cttcgacttc gcggcgcctc
     4801 gtcgctctcg cttccggagc tgcttgcgat tctgctcaga accgggagca gggggaagga
     4861 tgtcctcgaa ctcgctgcgg atgtgctcaa cgagtttgga ggcgcaaaag gaatggcgcg
     4921 ggctacggag gaagagatgc tcggttttcc cggactcggc gacgcgaagg cctgcgttct
     4981 ccttgccgcc ctggagcttg gaaagcgcct cgaagcggcg aggagagagc ttcaggaggg
     5041 aaaggactgg cgtcggagcg tcgacgagat agccggagag ctggctttcg aggagcggga
     5101 atatatcttt gccattcacg tccggagcaa cggatccgtg attcatgtgg acagaatttc
     5161 cttcggaggc gccgagggcg cctatcttga tgtaaaatat ctgttacgaa gagctgtccg
     5221 gatggattcg tacggcctgg tgctggtgca caaccatccc gacgggtcgc tgcagccgag
     5281 ccgcgaggat cgtctgctga ctgattttgt atcgaggagg acaaagatcc tggatataca
     5341 ccttttagga cattttgtcg tggccaacgg tgagagccat ggtatcgctc tccccggaga
     5401 gccctagaaa gcggggactg aagcaggtgt tcaaatttct tacgggagtg ttcggcggcg
     5461 acgtcggtat cgacctcggg actgccaaca tagttgtgta cgtcaagggg cggggaatcg
     5521 tcatcagcga gccgtccgcc gttgctattc ggaagcgccc cagaggaggg gcgacggaag
     5581 tgatcgccgt cggacgcgag gccaaggcga tggctggaaa aacgcccgga ggcgtcttca
     5641 ctatctggcc gctgcaggac ggcgtcatcg cgaacttcga aatgactgaa gaactgatcc
     5701 gtcactgtct caggagggcc aacgggggga aacccttcgt ctctcatccg cgggtcgtga
     5761 tctcagtgcc cgcggaggtt accgaagtcg agcggaaggc cgttatcgac gcaacgctcg
     5821 gggccggcgc caaggaggcg tacgtcatcg aggagcctgt tgcggcggcg ctcggcgtgg
     5881 gactgccgat ccaggaaccg cgagggagca tgatcctcga catcggcggg gggacgagcg
     5941 aagtcggcgt tctctctctc ggcggcatcg tcgtgaccaa ctcgctccgc gccgccggga
     6001 aggagatgga caacgccatt gtcgccatgc tccgccagcg atatgcgctg ctcatagggg
     6061 atacgacggc ggaggaggtc aagattgcca tcggatccgc gcttcccctc gacaaggagc
     6121 ttgagatggc cgtcaagggg cgcgacctcg cagatgggct tccgaaggcc gacgtcgtga
     6181 cctcggtgga ggtgcgcgag gcgctggagc cgattctgct gcgcatagag gacatggtga
     6241 aggtggcgct cgaacagacg cctcccgaac tggccaagga catcgtcgac caggggatct
     6301 ttctgtccgg cggcgtctcg ctgctgcgag gtctggccga acgcctgtcc aaggcgatga
     6361 atacccctgt ggtggtggcg gaggatcctc tcttcgcggt agcccgcggc gtggggaaga
     6421 ttctggagaa tctcggcgac atgaagaagg tgctcctctc cgtggagaag gggtccaagt
     6481 aatctgcgca tccggcggag agcctgctca tgacggaaaa atacagcgaa agagaatgga
     6541 ttcacggcct tttggcactt gccgccagtc ttctgctcct tgcggcggct ccccgaagag
     6601 agtttttccg gggggctgtt gatttcgtcg gagaggttct gcgctacccc gagtatcccg
     6661 ccgtcttcat cgagagaacg acgagggagt tctccgtctg gtttgccgac cgttacgttt
     6721 tacaggagcg cctcaatacc ctgaaagagg agaacgagcg cctgaatctc gtctggtcga
     6781 tggagaatgc ccgcaatctg cggagagaac tggac
//
LOCUS       DAOSWW010000024         6711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_7434_length_6711_cov_3.511719, whole genome shotgun sequence.
ACCESSION   DAOSWW010000024 DAOSWW010000000
VERSION     DAOSWW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7434_length_6711_cov_3.511719"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PK442_01005"
     CDS             complement(<1..839)
                     /locus_tag="PK442_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO86253.1"
                     /translation="MDLKRVGVIGVGHLGQHHARVYTELLGAHLVGVVDQNESRAAAI
                     GENLGVPWFTEIDDFIDRAAPEAVSVVVPTSMHFEIARKALKNGIHVLIEKPVTTTVN
                     EAEQLLRIAAESDLVLQVGHIERFNSAVQYISKIVHEPLFLQSRRLGPYSPRISDVGV
                     VLDLMIHDIDIILSLVRSEISDISATGRCIRSDHEDIASAQISFANGSMAHILVSRVS
                     ERRLRQLEIMEPERYVTIDYETQDVSINRCVRQNNNSLVEVIEHPVFPKSEPLKLELQ
                     HFVT"
     gene            complement(1679..3061)
                     /locus_tag="PK442_01010"
     CDS             complement(1679..3061)
                     /locus_tag="PK442_01010"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOO86254.1"
                     /translation="MMKRPFSTFEDLEDFFSRLASPGIRPGLERIARLLKQLGNPEKR
                     FSAIHIVGTNGKGSTAAFTERILRESGYTTALYTSPHLESPAERLLFSGVPLSLEEWS
                     ACAFEVEDALNDDPTLREDPPTFFEIVTATAFLLCEHRDVDIAVVEAGLGGRLDATNL
                     LGNICLTLITSISMDHMDFLGDTLESIAGEKFAVMRTRTPAVFSGTPAQLVPLFRSTA
                     NELGALPNTTAELCSCEEIRNESGRLSYVYRLLHPALLSSIGGEAKSFDQNAVIPVST
                     SLRGMYQIENSVLAISGILLLVSCFPKITSDTIRRGIAAARWPGRFETIDSTPPLVLD
                     GGHNPGGVEKLTESLSAVYGGKKIGVVYGCMKDKAYPECLELLRKVCSSLYCVSVPEN
                     PRAAQPELLAETARSHGWKPEDISVLHDPLEAIRVASLSNEVVVCCGSLYLIGYVKSR
                     LSFLKKERNE"
     gene            complement(3064..5748)
                     /locus_tag="PK442_01015"
     CDS             complement(3064..5748)
                     /locus_tag="PK442_01015"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOO86255.1"
                     /translation="MADIMESMAKSYDPEPIEDKWYARWIDSDLFKARVDKSKEAFSI
                     VIPPPNVTGSLHMGHAFNHTFQDIVCRFKRMRGYNVLWLPGTDHAGIATQNVVERRLA
                     GEGKSRHDLGRDEFVQRVWEWKDEFGSRIINQMKKLGNSCDWDRERFTLDEGLSKAVR
                     SVFVQLYKKGLIYKGKYIINWCSRCHTALSDIEVEHEECKGRLYYVRYPFVDGEGSVV
                     VATTRPETIWGDVAIAVHPRSEKGALVGRKVRVPLGGREIPIIEDIMVDPEFGTGCVK
                     ITPAHDPNDFLVGQRHNLEQVQVIDENGIMNSLAPGYEGLNIEEARKKAVEDLQASGH
                     LGTIEEIVHSVGHCYRCHTTVEPYLSEQWFVKAKPLAEAGVKVVQDGKIRWVPDQWEK
                     VYYQWMENIRDWCISRQLWWGHRIPAWTCGACGYITVSESDPTVCERCGSAAIVQDED
                     VLDTWFSSALWPFSTLGWPEKTEELAYFYPTSLMVTGFDIIFFWVARMIMMGLEFMDE
                     IPFHDVYIHALIRDEKGQKMSKSSGNVIDPLDMIEKYGADALRMTLAALTVQGRDILL
                     STSKIETYRLFMNKIWNASRFALMNLEGLPHEAVLSDDSELALHDRWILTRTSQVAGE
                     TTRLIEAFGIGEAARMLYDFVWGDLCDWYLELSKPALKGAEGPGRKLATQLVIASVFK
                     DVLLLMHPFIPFVTEELWQVFGFGKDAGGNSPFMERHPWPTGNDTFSFPESLEKMAAF
                     QEVVRVLRNLRAEARVTPQTFVNRAVLQCGEESSLSLIIHETAPLMQDLTKIREISLL
                     PLSADKPAGALSSQITGGEVSLIVGDILDIASEIARLSEEIASVTKNMQACSRKLENE
                     DFVRRAPADIVEKERLRLEENEARLRRIHENIESLKRS"
     gene            complement(5829..6428)
                     /gene="yihA"
                     /locus_tag="PK442_01020"
     CDS             complement(5829..6428)
                     /gene="yihA"
                     /locus_tag="PK442_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOO86256.1"
                     /translation="MNSKWKVSLECTAFTADQFPPPEIPEIAVAGRSNVGKSTLINNL
                     LGARLAHVGATPGKTRSVNFFLVEREGVPFRLVDLPGFGYASRSKAERNTWSRLIESY
                     VASRKSLWLVLHLVDFRHGLLENDASLQEWLDGIGLPVFVVFTKGDKIAKGKHLSTRD
                     QFIRKGMKSIDVPIITSGDKGTGIQELRVFIESFLDRQE"
     gene            complement(6425..>6711)
                     /locus_tag="PK442_01025"
     CDS             complement(6425..>6711)
                     /locus_tag="PK442_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOO86257.1"
                     /translation="MAVAILSAVRKSSVLPNIAMTGEVSLQGKVLPVGGIRDKILAAK
                     RQGIKNIILPLANKPDVEEIPEWSRDGLSFRYVDRVENVFEYALERAPSP"
BASE COUNT         1574 a         1896 c         1713 g         1528 t
ORIGIN      
        1 gttacaaaat gctgcaattc aagctttaac ggttcgctct tcgggaagac agggtgctca
       61 attacttcta ccaggctgtt gttattctga cgaacgcacc ggttgattga aacatcctgc
      121 gtttcgtagt cgatcgtgac ataccgttcc ggctccatga tttcaagctg acgcagcctt
      181 ctttcggaga cgcggcttac caggatatgc gccattgaac cgtttgcaaa ggaaatctgc
      241 gcggaggcga tatcctcatg atcggatcgt atgcatctgc cagtcgccga aatatcggag
      301 atttcggacc gaacgagcga aaggataata tctatatcat ggatcatcag atcaagaaca
      361 accccgacgt cgctgattct gggggaatag ggaccaagcc ggcgggattg aaggaagagc
      421 ggttcgtgaa ctatcttcga gatatactga acagcgctgt tgaaccgttc aatatgccct
      481 acctgaagca caagatccga ctcggcggcg attctgagca gctgctccgc ctcattgacc
      541 gtcgtggtca ccggtttttc gataagcacg tggattccgt ttttcaaggc ttttcgtgca
      601 atctcaaagt gcatgctggt tgggacgaca acgcttacgg cctcgggcgc cgcgcggtcg
      661 atgaagtcgt ctatttcagt aaaccatgga acgccgagat tttctccgat cgccgctgcc
      721 cggctttcat tctggtccac gacccccaca agatgggcgc cgagcagttc cgtgtagact
      781 cttgcgtgat gctggccaag atggcctact ccgatcacgc ctactcgttt caagtccatg
      841 gactcttcaa ctcctttgat tacgtacggc cttttcgtgc tcttacataa ttctcccggt
      901 aaacggagta aaaatggtat tttttactcc gtcaaaaaag cgaggagaaa aagtctgttt
      961 tccatatccc cgttcctgta ggagtagaat atatcgctcc tctcaaaggt acacagcgga
     1021 agggaacaga tattctcagg taagagcccc gcgtcggtca gttggcggaa aatttgccct
     1081 ccgatatcaa aagacacatc agcagaacgt acatctctgt tttcaggaga gaaaacagaa
     1141 agcccttttt ttgtccaagg gtcgttctgc tttctattat agctcctccg tccgattcct
     1201 ggccccaccc aggcatacgt tgcggcgggc cgacgtccgt tcgcagagaa gatgttttca
     1261 cagacagtac cgctgatgtt ggaaaccgcc cctttgaatc cggagtgcac tatggcgatc
     1321 cagggcgatg gcgccaagga agcaagaaca acggggaaac agtcggcaaa acgaagagat
     1381 ccctcgacac cgggagtgtc aagaaaaacg ccatcccctt ccggtcgttc aggtaaagca
     1441 tacgaaacct ccccgggaac catgaaagtg ccgtgcacct gatggggagc aatcaatgcc
     1501 ccttggcgcg gtattaacag ttcggtaagc tccctggccg tcgcgggatt cccgagagca
     1561 cgatccatgg ccgccccccg tagaaaaagg cggataaaaa aaggcgtatg cggctccgga
     1621 aagagatatt cgagaaacgg aaagtctcca ttgggctcga ttctgaaaga aggcgcgatc
     1681 attcgtttct ctcctttttg agaaaagaca atctgctctt tacgtatcca ataagataca
     1741 gactgccgca acagacgaca acttcgttcg aaagggaggc gacacgaatg gcttccaggg
     1801 ggtcgtgcaa aactgaaata tcttcaggct tccacccgtg tgaacgcgca gtctcggcga
     1861 gaagctcagg ctgagcagcg cgaggattct ccggaactga aacgcaatag aggctggagc
     1921 agacttttcg tagcagttcc agacactccg gatacgcttt atccttcata caaccgtaca
     1981 ccacgccgat cttctttccg ccgtagaccg cactgagact ctccgtcagc ttctctacgc
     2041 cgccgggatt gtgcccgccg tcaagaacca gcggaggggt tgagtctatc gtttcgaatc
     2101 tgcccggcca acgcgcagcg gcgattcccc ggcggatagt atcggatgta attttcggaa
     2161 aacacgatac gaggaggagg atccctgaaa tggcaagcac gctgttttcg atctgataca
     2221 taccgcgcaa ggacgttgaa accgggatga cggcattctg atcgaaggat ttagcttccc
     2281 cacctattga agacagcaac gcaggatgaa gcagacggta gacatacgac aatcttccac
     2341 tttcattgcg aatttcttcg cacgagcaga gttcggcagt ggtgttcggg agcgcgccaa
     2401 gttcattcgc ggtcgatcga aagagaggaa caagctgcgc aggagttccc gaaaagacag
     2461 cgggcgttct cgttcgcatt accgcaaact tttccccggc aatactttcg agagtatccc
     2521 ccagaaagtc catatgatcc attgaaatag acgtgatcaa cgtcaggcaa atattgccga
     2581 ggagattcgt cgcatccagt cttcccccca gaccggcttc gaccacggct atgtcgacgt
     2641 ccctgtgttc gcagagcaga aacgcagtcg ccgttacgat ttcaaagaac gtcggcgggt
     2701 cttcgcggag cgttggatca tcatttaagg catcttcgac ttcaaaagcg caggcagacc
     2761 actcttcaag cgagagagga accccgctga agagaagacg ttccgccgga gactcaagat
     2821 gcggactcgt atacaaagcc gtagtatatc ctgattcccg gaggatgcgc tctgtgaaag
     2881 cagcagttga tcctttgccg ttcgttccta cgatatggat ggcggaaaac cttttttccg
     2941 gattgcccag ctgcttaagc agacgggcaa tccgttcgag ccccggccgt attccggggc
     3001 tcgccaggcg ggaaaagaaa tcctccaaat cctcaaaggt cgagaacggg cgtttcatca
     3061 tctttaggag cgcttgaggc tttcgatatt ctcatgaata cgacgaagcc gagcttcgtt
     3121 ctcttcgaga cgaagacgtt ccttctcgac gatatccgcc ggagcgcgac ggacgaaatc
     3181 ttcgttctcg agctttcggc tgcaagcctg catgttcttt gtcacagacg ctatctcttc
     3241 cgaaagacgg gctatttctg aagcgatgtc gagaatatcc cctacaataa gactgacctc
     3301 tcctccggtt atctgggaag agagcgctcc tgcgggtttg tccgcggaga gcggcaggag
     3361 ggatatctca cggatttttg tcagatcctg cataagcggc gctgtttcat ggataatgag
     3421 ggacagcgaa ctctcctcgc cgcactgaag aacagcgcgg ttcacaaagg tctgcggcgt
     3481 caccctcgcc tctgcgcgaa ggttgcggag caccctgacg acttcctgga acgccgccat
     3541 cttctccagt gactcgggga aggagaatgt atcgttgcct gtcggccacg gatgacgttc
     3601 cataaacgga gagtttccgc cggcgtcttt tccaaacccg aaaacctgcc acaactcctc
     3661 cgtaacgaac ggaatgaagg ggtgcatgag aaggagtaca tctttaaaga cggatgcgat
     3721 gacaagctgc gtcgcaagtt tccggccagg accttccgcg cctttgagcg ccggttttga
     3781 aagctccaga taccagtcgc aaaggtctcc ccagacgaag tcgtacagca tccttgccgc
     3841 ctcgccgata ccgaaggctt caataagccg cgtcgtctct ccggcgacct ggctggtccg
     3901 ggtaagaatc catctgtcgt ggagcgcgag ttccgaatca tctgaaagga ctgcttcgtg
     3961 cggaaggccc tccagattca taagggcgaa acgcgacgca ttccaaattt tattcatgaa
     4021 gagcctgtat gtctctatct tgctggtgct cagcagaata tcccttccct gaacggtgag
     4081 cgcggcaagc gtcatccgga gagcatccgc accgtatttt tcaatcatgt ccagcggatc
     4141 tatgacgttc ccgctcgact tgctcatctt ctgccctttt tcatcgcgga taagcgcgtg
     4201 gatatagacg tcatggaacg gtatctcgtc catgaactcc aaccccatca tgatcatgcg
     4261 cgccacccag aagaagataa tatcgaagcc ggtcaccata agagaggtcg gatagaagta
     4321 cgcaagttcc tcggtctttt cgggccatcc gagcgttgaa aagggccaca gcgcgctgct
     4381 gaaccacgtg tcgagcacat cttcatcctg gactatagcg gcgcttccgc agcgctcgca
     4441 caccgtcgga tccgactcgg aaaccgtgat gtaaccgcac gctccacagg tccacgccgg
     4501 gattctgtgt ccccaccaga gctggcgtga gatgcaccag tctctaatat tctccatcca
     4561 ctggtagtag accttctccc actggtccgg cacccatcgt atctttccgt cctgtacgac
     4621 tttgactcca gcctcggcaa gcggcttcgc tttgacgaac cactgctcgg aaagataggg
     4681 ctcgaccgtg gtgtggcacc gatagcaatg ccccaccgag tgcacgatct cctctatcgt
     4741 accaaggtgt cccgaagcct gaagatcttc gactgctttt ttccgcgcct cttcaatgtt
     4801 cagcccctcg tatcccggag caagagagtt catgattccg ttttcgtcga taacctgcac
     4861 ctgttcaagg ttatgccgct gtcccaccag gaagtcgttg gggtcgtgcg caggagttat
     4921 cttgacgcac cccgtgccga actccgggtc caccatgatg tcctcgataa tcgggatttc
     4981 ccttccaccg agaggaacgc ggacctttct tccaacgagg gcgccttttt ccgagcgggg
     5041 atggactgcg atcgccacgt cgccccatat cgtctcagga cgtgtcgttg cgacgacaac
     5101 gctcccctcg ccgtcgacga aaggatagcg tacatagtaa aggcgccctt tacactcttc
     5161 gtgctcaacc tcgatatcgg agagcgccgt atggcaacgt gagcaccagt tgatgatgta
     5221 ctttcccttg taaatgagtc cttttttgta gagctgaacg aagacggaac ggacggcctt
     5281 cgagagcccc tcgtcgagcg taaaacgctc tctgtcccag tcgcaggagt tccccagctt
     5341 cttcatctgg ttgatgatcc gactaccgaa ctcgtccttc cactcccaga cccgctgtac
     5401 gaattcatcc ctgccgagat cgtggcgaga cttcccttcg cccgccagac ggcgttccac
     5461 gacgttctgc gtggcaatcc ctgcatggtc ggtaccggga agccagagga cgttataccc
     5521 gcgcatacgt ttgaagcggc agacaatatc ctggaacgta tgattgaagg catgccccat
     5581 atgaagtgat ccggtgacat tcggcggagg gattacgata gaaaacgcct ctttgctctt
     5641 gtcgacacgc gccttgaaaa gatccgaatc aatccagcgc gcataccatt tatcttcgat
     5701 cggttcgggg tcgtacgatt tcgccataga ttccataatg tctgccatgc tgcaattccc
     5761 ctccgttgcc taaaaatttg cttcctcggc agtatttcaa tcgaatcccc ctgcggggga
     5821 aacatctgtt actcctgacg gtcaagaaat gactcgataa ataccctaag ctcctgaatg
     5881 ccagtcccct tatcgcctga ggtgatgatg gggacgtcga tcgatttcat ccccttacgg
     5941 atgaactggt cccgcgtact caggtgcttt cctttcgcta ttttgtcccc ttttgtaaag
     6001 acaacgaaca cgggaaggcc tatgccatcg agccactcct gaagcgatgc atcattttcc
     6061 aggagaccgt gccggaaatc gacaagatgc agcacaagcc aaagactctt ccggctggca
     6121 acatacgatt cgataaggcg ggaccacgtg ttccgctccg ctttgctccg ggaggcatat
     6181 ccaaatcccg gaagatctac cagcctgaac gggactcctt cccgctcaac aagaaagaaa
     6241 ttgacgctcc gggtcttccc gggagtcgcc cccacatggg ccaaccgtgc cccgagcagg
     6301 ttgttgataa gcgtcgactt ccctacgttc gatcgtccgg ccaccgcgat ctccggaatc
     6361 tccggaggag gaaactggtc tgccgtaaac gcggtgcact cgagcgaaac tttccacttc
     6421 gagttcatgg agagggcgcc ctttcaaggg catactcaaa cacgttctcc acccggtcaa
     6481 cataacggaa agaaagacca tcccgcgacc actcaggtat ttcttcaaca tcaggcttgt
     6541 ttgccagagg aagaatgatg ttcttgatcc cctgtcgttt agcggccaat atcttgtccc
     6601 ggatacctcc gacggggaga acctttccct ggagagaaac ctctcctgtc atggcgatat
     6661 tgggaagtac cgagctcttg cgaactgcgg aaagaatagc cacggccatg g
//
LOCUS       DAOSWW010000025         6682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_7479_length_6682_cov_3.738494, whole genome shotgun sequence.
ACCESSION   DAOSWW010000025 DAOSWW010000000
VERSION     DAOSWW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7479_length_6682_cov_3.738494"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PK442_01030"
     CDS             complement(<1..671)
                     /locus_tag="PK442_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOO86258.1"
                     /translation="MDYRIADIALAPSGEKKIGWARQYMPVLRHLEKKYKAEQPLAGT
                     VISACLHLEAKTACLLLLLKDLGAKVVAAGSNPLSTQDDVCAALVKNGVAVFSRNGMA
                     TDEYFENACSALSVGPNIVVDDGADLVALLHTEMKHLLPGIRGGSEETTSGVKRLRSM
                     ANEGILAFPMISVNDAHSKYLFDNRYGTGQSVMDGIMRTTNMLVAGKNVVIAGYGWCG
                     RGVAMR"
     gene            complement(686..1705)
                     /gene="mtnA"
                     /locus_tag="PK442_01035"
     CDS             complement(686..1705)
                     /gene="mtnA"
                     /locus_tag="PK442_01035"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOO86259.1"
                     /translation="MLPRVISWEENRLSLLDQRRLPSSVEFLKCEAAEEVAVAIETLA
                     VRGAPAIGIAAAFGVALEARRGRAEALRAVQRLAVTRPTAVNLFWALRRVEERMKTVP
                     AEASNETLFSAVLEEATAIQREDLEGNRALGRAGAALLPDDCTVMTHCNAGALATGGH
                     GTALGVIRSAREAGKTVRVYACETRPLLQGARLTVWELMQDGFDVTLLCDNMAASLMK
                     SRRIDAVIVGADRIAATGDTANKIGTYSHALLAKHFGIPFYVAAPLSTFDVSLPSGER
                     IPIEERAADEVRRLPGGGMLPADYSIWNPAFDVTDASLISAIITEKGAIVPPFTEGIA
                     SFLSE"
     gene            complement(1726..2964)
                     /gene="xseA"
                     /locus_tag="PK442_01040"
     CDS             complement(1726..2964)
                     /gene="xseA"
                     /locus_tag="PK442_01040"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOO86260.1"
                     /translation="MMKRMPTTLTVDEISLRIASLFLKDPVMQSVGVRGELSELKKHS
                     SGHVYFTLLGKESRISCALFRSYASYVPEWPRNGDEVVAEGAVSVYAPRGGYQLLVKR
                     LVPLGKGAADRARLELMARLEAEGLFSPGLKRPFPSYPARVAVVTSPTGAALRDVIAV
                     ASKRMPSCELVIIPAAVQGYEAPEAIAEALGRIGSVAGAECAMLVRGGGSRDDLSAFD
                     DERVVRAVRNSPVPLVTGVGHEIDETLSDLAADLHAPTPSAAAERIFPDRRDILRLLD
                     HRLASARAALELDMSKSLRGIDALLSRGARTLEKDIMTRNASLQLSSSRLFSAAERAL
                     SESRERLAACAASLDGLSPLKAFDRGYIVCEKKGVPVLRAAELSSKDEVCLRFRDGDA
                     NAKIRSSSVRPVSQGDAADS"
     gene            complement(2930..3421)
                     /gene="nusB"
                     /locus_tag="PK442_01045"
     CDS             complement(2930..3421)
                     /gene="nusB"
                     /locus_tag="PK442_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOO86261.1"
                     /translation="MNRGFLPQKRHRAREMALQLLYALDLRRSQTPEQAFELFPGDED
                     PEILEYARVLTSGAWERRMDIDNLIREYVVGWRPERMVAVDRAALRMCIYESLFAKCT
                     PVAVAISEAVELAKTFGTEESGKFVNGVLGRIVRNLPPDAAAQDASAFDAGDETHADD
                     ADS"
     gene            complement(3426..3908)
                     /locus_tag="PK442_01050"
     CDS             complement(3426..3908)
                     /locus_tag="PK442_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86262.1"
                     /translation="MYLFWKRTSLGSILVSQDGMCDFVNSLCEKPFKCAQVSLSSADN
                     ALFLVITMPKDYDSSAMVSLETKLRDTFEKTGLSVRVSWVELAKTSFVDNEALASLAK
                     KPVVWAIAVALFALLLKEGVATLLWSLFWGGAFYAGVLFFQSEMGAELVRKLRGMAGR
                     "
     gene            complement(4009..4560)
                     /locus_tag="PK442_01055"
     CDS             complement(4009..4560)
                     /locus_tag="PK442_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOO86263.1"
                     /translation="MDEMNKKLESEVLEEESAYEGVLGETGESEAVPEGMVAGVVHIS
                     EEVLSELAKKTLSSIDGVQPASPGIASKLGIGRKASEGVRVVLEDNGLHPMASVDVYI
                     LVKFGLRIPDLAWDVQEMIKKNIEEYTGYKVKAVNVNVQGIYFKNKVPAEAVAEEAKG
                     EVKQPEDSVPELDKSVQPEVEQL"
     gene            complement(4632..5060)
                     /locus_tag="PK442_01060"
     CDS             complement(4632..5060)
                     /locus_tag="PK442_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86264.1"
                     /translation="MSAREKIAYLKGLLDGLGPVENEGQGKIFRAVVDALDALSLEVA
                     EHEDLIQEQRELYEDLADDCALLDEDLDSLEKAFSDFSGEGDMDDEDDEDEDDEFDRN
                     YVSVTCPHCSYVFYYQPEEYEPDEALECPGCGKDFEQPKE"
     gene            complement(5081..5653)
                     /gene="efp"
                     /locus_tag="PK442_01065"
     CDS             complement(5081..5653)
                     /gene="efp"
                     /locus_tag="PK442_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOO86265.1"
                     /translation="MAQVVDTSDFYSGIKIRWQEGIWEVIDFQHHKMGRGGATVRTKM
                     KNLDTGTIIENSFRSGERFERIIFDEKPAQFSYKEGDSYIFMDLSTYDQLSIPADILG
                     NVVNFLMDDMEVQIEFFEGRIMGIEMPKSVELKVVDTPPSFKGDTVSGGGKPAKLETG
                     ITISVPIFVNVDDTIVVDTRSGQYLERAKK"
     gene            complement(5773..>6682)
                     /locus_tag="PK442_01070"
     CDS             complement(5773..>6682)
                     /locus_tag="PK442_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HOO86266.1"
                     /translation="LVQSLAGVSNIVLDERLRTLYVTSRPEQFPHVERVLQEVDHPGR
                     QVMLQARIIEVTDAGRDQLETIIDAVYNQWWLNFSSGGMNLGYLYADKPGAYKPGDER
                     PAPGIDDGLESIAKGDLTKMLDFGLNSIVTANKGKVLADPSIITLDGKKAVIKLVENY
                     PYISERDQAGNPTWSEKEVGPILEFTPTVGRDQMVTVELKIQTGEIIGTYKGQAGEEF
                     PQTSNREVTTMIRVRDGEPFVVGGLHKDVKRTERGRVPILSNIPLLGELFKYRKDIRD
                     KSEVAMIVIPYILTTPDGPVEQFTLK"
BASE COUNT         1430 a         2161 c         1716 g         1375 t
ORIGIN      
        1 cgcatcgcca caccgcgacc gcaccagccg taccccgcga tgaccacgtt ctttccagca
       61 accagcatgt tcgtcgtccg catgatgccg tccatcaccg actgccccgt cccgtagcgg
      121 ttatcgaaga gatacttgct gtgcgcgtcg ttcaccgaga tcatcgggaa ggccagaatt
      181 ccctcgttcg ccatcgaacg caggcgcttc actccggacg tcgtctcctc ggagcctcca
      241 cggataccgg gaagcagatg cttcatctcg gtgtgcagca gcgccacaag gtcggcgccg
      301 tcgtccacca cgatgttcgg gccgactgaa agcgctgaac aggcgttctc gaaatactcg
      361 tccgtcgcca tgccgtttct gctgaagacg gccacgccgt tcttcacgag cgccgcgcac
      421 acgtcgtcct gggtggaaag cggattgctc cccgcagcga caactttcgc accaaggtcc
      481 ttcagcaaca gcagcaggca ggccgtcttc gcctccaggt gcaggcacgc ggagatcacc
      541 gttccggcaa gcggctgctc ggctttgtac ttcttctcaa gatgacggag cacaggcata
      601 tactgccggg cccacccgat tttcttctcg cccgaaggcg cgagcgcgat atccgcaata
      661 cgatagtcca tgtgtgttct tcctcctatt cagaaaggaa cgaggcgata ccctctgtaa
      721 acggaggaac tatcgcgccc ttttccgtaa taatagccga aatcagcgaa gcgtcggtaa
      781 cgtcgaacgc cggattccag atcgaatagt ctgcaggcag catcccgccg ccggggagcc
      841 tgcgtacctc gtcggccgcg cgctcctcta tcggaatgcg ctcgccggaa ggcaacgaga
      901 catcgaacgt gctcaacggc gcggcaacgt agaatggaat accgaagtgc ttcgccagca
      961 gcgcatgcga ataggtcccg atcttattcg ccgtgtcgcc ggtggccgcg attctatccg
     1021 cgcccacgat caccgcgtcg attcgcctgc tcttcataag gcttgccgcc atattatcac
     1081 acaacagcgt cacgtcgaaa ccgtcctgca tcaactccca gacggtgagt cgcgccccct
     1141 ggagcagcgg acgggtctcg cacgcataca cgcgcacagt tttccccgcc tcccgagccg
     1201 aacggattac tccgagggcg gtgccgtgtc ctccggtcgc cagcgctccc gcgttgcagt
     1261 gcgtcatgac ggtgcagtcg tccggaagca gcgcggcgcc cgcgcggcca agggcgcggt
     1321 tcccctccag atcctccctc tgaatagcag tcgcttcctc cagaacggcc gaaaagagcg
     1381 tttcgttcga agcctccgcc ggaacggtct tcattcgttc ctcaacgcgc cgaagcgccc
     1441 agaagaggtt caccgcggtc ggacgcgtta cggcaagacg ctgaacggcg cgaagcgcct
     1501 ccgctcgccc tctgcgggct tccagcgcga cgccgaaggc cgcggcaata ccaatcgcag
     1561 gcgcgcctcg cacagcgagc gtttctatcg ctacggcgac ctcctcggcg gcttcacact
     1621 tcaaaaactc tacgctcgac ggcagcctcc tctggtcaag aagagacagt ctgttttctt
     1681 cccatgaaat aacccggggc agcatgtcac ccctccttct ttacgttagg aatccgcagc
     1741 atccccttgg gaaacagggc gcacgctgga agaacggatc ttcgcgttcg catccccgtc
     1801 gcggaaccgg agacacactt cgtctttgga tgagagctcg gccgctctga gcacggggac
     1861 gcccttcttc tcgcagacta tatacccccg gtcgaaagct ttcaacggag agagcccgtc
     1921 gagagaagcg gcgcacgcgg ccagccgctc tctggactcc gagagcgccc gctccgccgc
     1981 cgaaaaaagt ctgctcgaag agagctggag cgatgcgttc ctcgtcatga tgtccttctc
     2041 aagagttctc gccccgcgtg aaagcagcgc atcgatgccg cgcagactct tcgacatgtc
     2101 aagttcgagg gccgcccgcg ccgatgcaag ccggtgatcg aggagacgaa gaatatcccg
     2161 ccggtcgggg aaaatacgct cggcagctgc ggaaggggtc ggcgcatgaa gatcggccgc
     2221 caggtcggag agtgtttcgt cgatctcatg ccccactcca gtcaccagcg gaacaggaga
     2281 attgcggaca gcccggacga ctctttcgtc gtcgaaggcc gacaggtcgt ccctgcttcc
     2341 gcctccccgg accaacatgg cgcactccgc ccccgcgaca gacccgatac gccccaacgc
     2401 ttcggcgatg gcctcaggcg cttcataccc ctgcaccgcc gcggggataa tcaccagctc
     2461 gcaggaaggc atccgcttgg aagccaccgc gatcacgtcg cgaagcgcgg cccccgtcgg
     2521 cgacgtcacc acggcgacac gcgcagggta ggagggaaac ggacgtttca aacccgggga
     2581 gaaaagccct tccgcctcaa ggcgcgccat cagctccaac cgtgcgcggt ccgcggcgcc
     2641 cttgcccaac ggtaccagcc gtttcacaag gagctgatac cctccgcggg gagcatagac
     2701 ggaaaccgcc ccctcggcga ccacctcgtc cccgtttcgg ggccactcgg ggacatacga
     2761 agcataactt ctgaaaagag cgcacgaaat acggctttcc ttccccagga gggtgaaata
     2821 gacatgaccg ctggaatgct tcttcagttc gctcaactcg ccgcgaacgc caacggactg
     2881 cataacgggg tccttcagaa aaagcgaggc gatacggagg gaaatctcgt caactgtcag
     2941 cgtcgtcggc atgcgtttca tcaccagcgt caaatgcgga cgcatcctgc gcagccgcat
     3001 caggaggaag attccggaca atacgcccca gaacgccgtt gacgaacttg ccggattcct
     3061 cggtaccgaa ggtcttcgcc agttcgacgg cctccgaaat tgcgaccgcg acgggagtgc
     3121 attttgcgaa caggctttca taaatgcaca ttctcagagc cgccctgtcc accgcgacca
     3181 tgcgttcggg acgccatcca acaacgtatt cacggatcag attgtctata tccatacggc
     3241 gctcccatgc gccggaagtc aaaacgcgcg cgtactccaa aatctccgga tcctcgtcgc
     3301 ctgggaacag ctcgaacgcc tgttccgggg tttggcttct ccggagatca agcgcataca
     3361 ataattgaag cgccatctca cgtgcacgat gacgcttctg cggcaaaaaa ccacgattca
     3421 ctccattacc tccccgccat gcccctcagc tttcgcacaa gttcagcgcc catctccgac
     3481 tggaagaaaa gaactcccgc gtaaaatgcg ccgccccaga aaagactcca tagaagcgtg
     3541 gccacgccct ccttgagcaa gagggcaaaa agggcgacgg caatcgccca gacgacaggt
     3601 tttttcgcaa gcgaggcaag cgcctcatta tccacgaacg acgtcttcgc aagctccacc
     3661 catgacaccc gcacagaaag gccggtcttc tcgaaagtat cccgcagttt tgtctccaga
     3721 gaaaccatag cggatgaatc gtaatccttc ggcatggtga tgacaagaaa aagagcgttg
     3781 tcggcagagg aaagagagac ctgcgcgcat ttgaacggct tttcgcacaa agaattgaca
     3841 aagtcgcaca ttccatcctg cgaaaccagg atactgccga gggaagttcg tttccagaaa
     3901 agatacattc gcatcacccc tgttcgaagg gggctctgaa actgaaggag cccccttcaa
     3961 ggttcgttga tgaactatga actgaaaaaa accgcatcga agcgaatgtc agagctgctc
     4021 cacttccggc tggaccgatt tatcaagctc cggaacggaa tcctcgggct gtttcacctc
     4081 gcccttcgcc tcttcggcaa cggcttcggc gggaaccttg tttttaaagt agatcccctg
     4141 gacgttcacg ttcaccgctt tcaccttgta gccggtgtac tcttcgatgt tcttcttgat
     4201 catctcctga acatcccatg caagatcggg aatgcgaaga ccgaacttca caagtatata
     4261 cacatccacg ctcgccatag gatggagccc gttatcctca agcacaaccc gaacgccttc
     4321 gctcgctttt cttccgatgc cgagcttgga ggcgataccg gggctggcgg gctgcacacc
     4381 gtcaatggag gaaagcgtct tcttcgcaag ttcggagagc acctcttcgc tgatatgcac
     4441 tacgccggca accataccct ccggaacagc ctcgctctcc ccagtctcgc ccaaaacgcc
     4501 ttcatacgca gattcttctt caagaacttc agattccagc tttttgttca tttcatccat
     4561 ttcgagacct cccttccagt tttacacgcc cggccggcag cgaattaaag ctcccggccg
     4621 aatgcttccg tctattcttt gggctgctcg aaatccttcc cgcagccagg gcattcaaga
     4681 gcctcgtccg gttcgtactc ctcgggctga tagtagaaga cgtagctgca atgagggcac
     4741 gttacggaaa cgtaattccg gtcgaactcg tcgtcctcgt cttcgtcatc ctcgtcgtcc
     4801 atgtcgcctt cgccggagaa atcggagaac gccttttcca gcgaatcaag atcttcgtca
     4861 agaagcgcgc agtcgtccgc caagtcctcg tacagctccc gctgctcctg aatcagatcc
     4921 tcgtgctccg ccacctccag agagagcgcg tcgagcgcat ccacaacagc cctgaaaatc
     4981 tttccctgtc cttcgttctc gaccggacca agaccgtcga gcaacccctt gagataggct
     5041 atcttttccc gtgcgctcat ctcgtcaacc tccctgcgtt ttattttttc gcccgttcaa
     5101 ggtactgacc cgagcgggtg tcgacgacta ttgtatcatc tacattgacg aaaataggaa
     5161 ctgaaatggt aatccctgtt tcgagcttcg ccggtttgcc tccgccggac accgtatcgc
     5221 ccttgaagct tggaggcgtg tcgacgacct tgagttcaac gctcttcggc atctcgatgc
     5281 ccattatgcg tccctcgaaa aactcgatct ggacttccat atcgtccatg aggaagttca
     5341 cgacgttgcc cagaatatcc gcaggaatag agagctggtc ataggtcgag agatccatga
     5401 aaatatagct gtcgccctct ttgtaactga actgcgcagg cttctcgtcg aagatgattc
     5461 gctcgaagcg ctcgccggag cggaaagagt tttcgatgat cgtccccgtg tccaggttct
     5521 tcatcttcgt cctgacggtc gctcctccgc gccccatctt gtgatgctgg aaatcaatga
     5581 cttcccatat accttcctgc cagcggattt ttatccccga ataaaagtcc gacgtatcca
     5641 caacctgcgc cataacactc tacctcccaa tattggttca aaacgatcaa aaaaactgca
     5701 tctactgcaa cgcaggtata ttatcatatc cggaagtcct tctcaaaaaa ccgctgcaaa
     5761 aaaatcaacg cgctatttta aagtgaattg ctccaccgga ccgtcgggcg tcgtcagaat
     5821 gtacggaatc acgatcatcg ccacctcgga cttgtcccgg atatccttcc tgtatttgaa
     5881 cagctctccc agaaggggga tgttgctcaa tataggcaca cgtccgcgtt cggtccgctt
     5941 cacgtcctta tgcagaccgc cgacaacgaa cggctcgccg tcgcgaaccc gtatcatcgt
     6001 ggtcacttcc cggttggacg tctgcgggaa ctcctcccct gcctgccctt tgtaggtgcc
     6061 gataatttcc ccggtttgaa tcttgagctc gaccgtcacc atctggtcgc gccccacagt
     6121 cggagtgaat tcaaggatgg ggcctacttc cttctcgctc cacgtcgggt tgcccgcctg
     6181 atcgcgctcg gagatgtagg ggtagttctc cacaagcttg atgacggctt tctttccgtc
     6241 gagcgtgata atagacgggt cggcaagcac cttccccttg ttggccgtca cgatgctgtt
     6301 cagcccgaag tcgagcatct tcgtcaagtc gcctttcgcg atgctctcca gtccatcgtc
     6361 gatccccggc gcgggacgtt catcgcccgg tttgtacgcc cccggcttat cggcgtagag
     6421 atacccgaga ttcatcccgc cggagctgaa gttgagccac cactggttgt acaccgcgtc
     6481 gatgattgtt tcgagctgat cgcgccccgc atcggttact tcaataatac gcgcctgcag
     6541 cattacctgg cgccccggat gatcgacttc ctggagcacg cgctccacat gcgggaactg
     6601 ctccggcctg ctcgtcacgt agagtgtgcg cagacgctcg tccaacacga tattggagac
     6661 tccggcaagg ctttgcacaa gc
//
LOCUS       DAOSWW010000026         6603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_7609_length_6603_cov_4.118357, whole genome shotgun sequence.
ACCESSION   DAOSWW010000026 DAOSWW010000000
VERSION     DAOSWW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7609_length_6603_cov_4.118357"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK442_01075"
     CDS             <1..454
                     /locus_tag="PK442_01075"
                     /inference="COORDINATES: protein motif:HMM:NF036996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86267.1"
                     /translation="VLHEEYLVNVHSLELETSIFLVTLFPGFRSRYLSHLADAGPKAI
                     VLEALGLGGVPYLGENLLPAIAMCRERSIPVIITTQCVYGGVDLSVYEVGRKTLELGA
                     ISGKDMTRETIITKLMAVLPGTDNKQIENILHCNFCDEIEEPECPADA"
     gene            complement(1384..1911)
                     /locus_tag="PK442_01080"
     CDS             complement(1384..1911)
                     /locus_tag="PK442_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOO86268.1"
                     /translation="MENGIDPRALFTLNYGVYILSTGYEGKYNGQIINALMQLTADPI
                     CMAACLHKDNYTTELVEKSGRFSVSVLEESVPLKFIGVFGFRCGRDFDKFGECRYEVK
                     EGGLPLVTEYSLAGIELKVLSVQDVFTHKVFIGQVEKAQLFKEGTPLTYANYHRVKKG
                     KSPSNAPSAIFNEVK"
     gene            complement(2005..2499)
                     /locus_tag="PK442_01085"
     CDS             complement(2005..2499)
                     /locus_tag="PK442_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOO86269.1"
                     /translation="MISKKIEDAINDQINAELYSAYIYLSMSSWFDSANLKGMSHWMG
                     IQAKEEVEHAMKFAGYVMERGGRVLYKAVEGPETEWTSPLEAFKVAYEHEKYVTRRIN
                     DLMDLAVAEKDYASQALLQWFVTEQVEEEANADEIVSKLEMIGEGKHGLLMIDRELGG
                     REDD"
     gene            complement(2502..2864)
                     /locus_tag="PK442_01090"
     CDS             complement(2502..2864)
                     /locus_tag="PK442_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOO86270.1"
                     /translation="MKKLDIFKCELCGNVVELLHVGGGDLVCCGQPMKLLDEQSADSA
                     TEKHVPVIEGNKVKVGSVPHPMTEQHYIEFIEIMAGDKLCRKFLNPGEAAEAVFEGFV
                     PEKSREYCNIHGLWKAEK"
     gene            3112..4119
                     /locus_tag="PK442_01095"
     CDS             3112..4119
                     /locus_tag="PK442_01095"
                     /inference="COORDINATES: protein motif:HMM:NF027852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgT C-terminal domain-containing protein"
                     /protein_id="HOO86271.1"
                     /translation="MQSQSSIIDRFFFVLLLIIVMYACQSEAASSRHIVLLPTLNYTE
                     YEIWESKYYPVNVLEQKMTEYLASLLRQNPFTDVSILDSSQAQRWMDDPYSRQGAFAV
                     QMELYSVLAKEREVLGSFEKGDVSLRVRIYSALDAELETSYISSGKDQRFTFNPGDDR
                     LYLLNTRTSIIDIFYRNGLDFLRLAPADKGQKMSRLTWQQFSGTPYWQAFKNAIKGVA
                     TGIADMGDGELAVIGRIIAPTANSTRRNREYIITLGRQDALAVGDILQVVRGDTYVTV
                     DPENPVVVLQRVVGNVKVIKTMNEQAIVRLLNEKSQDPVQLNDLVTAPPYGTKKVAPL
                     R"
     gene            complement(4723..4938)
                     /locus_tag="PK442_01100"
     CDS             complement(4723..4938)
                     /locus_tag="PK442_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86272.1"
                     /translation="MKDSPSISRERDWEACLAALHELERRRLVKWNCATAAGHRVSAE
                     PGGCTVLVAEEPEEQEGRILLAAVPVQ"
     gene            5252..6034
                     /locus_tag="PK442_01105"
     CDS             5252..6034
                     /locus_tag="PK442_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86273.1"
                     /translation="MRRRILIVLSLTVLLSLLPSSTTATTYVVEPVLVAQPVYSGMSF
                     YVYRPYNMPSGWFVTFDGYPITRTPAGVWVYGSYNGSLLAPTSFVVGSISPQSLPIVQ
                     YVPSARVSSTQSLPVTPLPPLPAPIQPDPGAYILAEAVTVAPTYVPEWTTQSSFTSIG
                     TWNKLVDRMGILEKPRVPIAWKGERPEALFVWTGKSWYQIQARSGVSEKPSEMIKDHL
                     YALTRMVKNNAYHWKDADTAILANQAAVWGYLWMGRIAPSNL"
     gene            6098..6493
                     /locus_tag="PK442_01110"
     CDS             6098..6493
                     /locus_tag="PK442_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86274.1"
                     /translation="MKKSFRYIFFLFSYTFTFLFFFPGASSANYVLIPAQAPAIITTP
                     DYVDSFQAPSPSALSGQIFRSKEGYLFSFDASGRRVYHMHASYVLSAHGSSPVPVHII
                     AAPMITVHPGAVLTPSPYTYLPQQYLLVP"
BASE COUNT         1637 a         1772 c         1470 g         1724 t
ORIGIN      
        1 tgttttgcat gaggaatact tggtaaatgt gcactctctt gaactggaga cctcgatttt
       61 ccttgtaacg ctctttcccg gttttcgttc ccggtacctc tctcatcttg ccgacgccgg
      121 gccaaaggcc atagttctcg aagcgctcgg tctgggagga gttccgtatc tgggtgaaaa
      181 cctgctcccg gccatcgcta tgtgccgcga gcgaagcatc ccggtcatta ttacgacgca
      241 gtgcgtttac ggaggagtcg atctcagtgt gtacgaagtc ggaagaaaaa cgctcgaact
      301 gggcgccatc tccggaaagg acatgacccg ggagacaatc atcacgaaac tgatggcagt
      361 tttacccggg accgacaaca agcagatcga gaacattctt cactgtaatt tttgcgacga
      421 aatagaagaa ccggaatgcc cggcggatgc gtagtttaac gctccctggc atttctcaag
      481 aaaggagggt tcgctcagct ccttccgtat accgaatctc gtaaggacga aatcaaattt
      541 tgtaggatcc tccggaagaa tctcccgaaa aagtgcggtt acttcaatcg cagttttcag
      601 atccggttgt ttgcgtcgtg tcattccaag ggttgagcag atccggaaca tatgtacgtc
      661 cattggaata aggagatccc gcggtttcag ggctgtccat ccgccgggat cgacctcgtc
      721 ccttcggacc atccatttga gaaaaagaaa gaatcgtttg catgcgctcc catcactcgg
      781 agaaggcaga agaaaacggc tttcaaggga actctttctt cgtacgttct caacaaaatt
      841 tgaaattcct tcgagaacat ctcccgtcct gctcaaagaa actgcaagaa actcctctac
      901 gcttccatat tctcgaagaa tacgccctat cccggtcatt agcgccgcga tctccgctcc
      961 ggatgtgaag cgatggcgaa ataattctgt ttcacgagca agaacaagag gcgaaacagt
     1021 cttgaggaat tccgcgggcg atggaccgag gagttcaaga agacgccggg cgcttcgaag
     1081 gatcatcgca acacgcccgt acgctatact tgagacgagg agcgccgcaa cctccctgtc
     1141 ctcggtacga gagtaagggt agatagtctc cagaggatcg ggagaaacaa attcacgccg
     1201 gttgtatcgg gcgtatgctt cttcgaaaca gggccatatt cgatgaagag cgctcgcttc
     1261 tctatttttt tccgtcattg cactccccga aaaataaacc aggaaccgtg agagagaaaa
     1321 acctacttcc cgcacctcac agttccgtgg aaaaattaac cattcgtttt cagtcttcgg
     1381 tcgtcatttg acttcgttga atatcgcgga cggagcattc gatggagatt tacctttttt
     1441 cactctatgg tagttcgcgt aggtcagagg ggttccttct ttaaaaagct gcgctttctc
     1501 cacctggccg atgaagacct tatgtgtgaa gacatcttgt acggagagaa ctttcagttc
     1561 tatacccgcc agagaatact ccgttacaag aggaagaccg ccctctttca cttcgtacct
     1621 gcattccccg aatttatcga aatcgcgtcc gcaacgaaaa ccgaagacgc cgatgaactt
     1681 caaaggaact gattcttcga gcacggagac ggagaaacgc ccgctcttct ccacgagctc
     1741 cgttgtgtag ttatccttgt gaaggcaagc tgccatgcag atcgggtcag cggtaagctg
     1801 cataagcgcg ttgatgatct gtccgttgta tttgccttca tacccggtac tgagaatgta
     1861 cactccataa ttcaatgtaa aaagagcccg ggggtcgatc ccgttttcca ttgagtttcc
     1921 tcccttcatc ttcctgcact gcgggcagga gcctgaagct cctgcccgca gattgtcttc
     1981 actaaggaga tgcttcaagg acttctagtc gtcctctctg ccacccaatt ctctgtcgat
     2041 catcaggagg ccgtgcttcc cttcgccaat catctcaagc ttgctcacga tttcgtccgc
     2101 attcgcctct tcttcgacct gctccgtgac aaaccactgg agaagcgcct ggcttgcata
     2161 atccttctcg gcaacggcga gatccatgag gtcgttgatc cggcgggtca catacttctc
     2221 atgttcgtac gcaactttaa aggcttccaa aggagaagtc cactcggttt ccggtccctc
     2281 cacagcttta tacaggactc ttccgccgcg ctccattaca tagccggcga atttcatcgc
     2341 atgctctact tcttccttcg cctggattcc catccagtga gacattccct tcaaattcgc
     2401 cgaatcgaac caagacgaca tcgaaaggta gatatacgca gagtaaagtt ccgcattgat
     2461 ctgatcgttt atcgcgtctt caattttttt actgatcatg cttacttttc cgccttccat
     2521 aaaccatgta tattgcagta ttccctgctc ttctcaggaa caaatccctc aaaaacagcc
     2581 tctgcggctt cacccgggtt caggaacttt ctgcatagtt tgtctcctgc cataatttca
     2641 atgaactcaa tatagtgctg ctcagtcatt ggatgaggaa cgctgccaac cttcaccttg
     2701 ttaccttcga tgaccggcac gtgtttctcc gttgcagaat ccgccgattg ctcatcaagg
     2761 agcttcatgg gctgaccgca acagacaagg tcgccccctc cgacgtgaag cagttccacg
     2821 acattgccgc acagctcaca cttaaagata tccagctttt tcattcagca tctttcctcc
     2881 tttgaaatcg ttttttttct tgaaaacaga tttattatat caccttccaa gtcaattctc
     2941 atcctctcca cccttgaatc agaatatgct ctataatagg aagcgggaag aagacggaga
     3001 gcccatctct ccgaatgcct ccttccttgg ctgtcccttt accggtagta tcttctgccg
     3061 atacattatt gagagcagcc tggactccat ttgaaaagag gatgagaacc aatgcagtct
     3121 caatcatcaa taatagaccg ttttttcttc gttttactgc tgattatcgt gatgtacgca
     3181 tgtcaatccg aagccgcttc gtcacgtcat atcgttttgc ttcccacgtt gaattatacg
     3241 gagtatgaaa tttgggagag taagtactac ccagtgaatg tgcttgaaca gaagatgact
     3301 gagtatctgg cctctctcct gcggcaaaac ccctttacgg atgtttcgat actggatagc
     3361 tcgcaggcgc aacgctggat ggatgatcct tactccaggc aaggcgcttt cgcagtgcaa
     3421 atggaactct actcggtact cgcaaaggag cgcgaggttc tcggttcttt tgaaaaaggt
     3481 gatgtgtcct tgcgagtcag aatttacagc gctcttgatg cggagcttga aacttcctat
     3541 atttcctcgg ggaaagacca gcgattcacg ttcaatccag gcgacgacag gttgtatctg
     3601 ctgaacacac gaacgagcat tatcgatatt ttctatagaa acggccttga tttccttcga
     3661 cttgccccgg cagataaggg gcagaaaatg agtcgtctta catggcaaca gttttccggc
     3721 accccttatt ggcaagcttt taagaacgcc attaagggag tggctacagg aatagccgat
     3781 atgggggacg gcgaactcgc tgttataggg cgcattattg cccctacggc aaactcgaca
     3841 cgacgcaaca gagagtacat aataactctc ggtcgacagg acgctctcgc tgtcggcgac
     3901 atacttcagg tagtcagagg cgatacatac gtaacggtcg accctgaaaa ccccgttgtg
     3961 gttttacaga gagttgtagg caacgtcaag gtgatcaaaa ccatgaacga acaggctatc
     4021 gttcgcctgc tcaatgaaaa atcacaagac cccgttcagt tgaacgatct tgtaacagct
     4081 cctccatacg ggacaaaaaa ggtggcgcct ttgcgatgaa caaaaaaaaa ctactccaca
     4141 tatttctgct ctttctgttg tttttttcgt cccatatttc tttcacgcgt ttgcagtcag
     4201 cgcatgcaaa cggagtgatt gtgactgcgc ctgagaatgc acaacaaaaa gccgcgccca
     4261 agaagaaacc ctccgtgaaa aaacaggtgc aaccaacgcc tccgccaatc caggaggaaa
     4321 aagaggtaat agtgcttccc acgcctttag atcaggcgct ggttcttatg gaaaagagat
     4381 tctttacgag agcaactgcc ctattggaac agattgtaga acgcgaaccg ttgaacgcac
     4441 atgcttggta cgcactcgga agagcatatg aggcgcgggg gttttttcca aaagcccaga
     4501 aagctcttcg cagatcactg gaaatagacc cttcttttaa cgagttgtct cgttttttgg
     4561 agtatcctgc agaaggggac agacagccct tgtgggatcc caaacgtccg gcgagaatag
     4621 aggagattcc tgttgctatt gacggtttta caatcatgcc tccttcggag actcttgttc
     4681 ctccggtgag tccagtacaa ccgcctattc aatacgcgtc tcctactgca ccggtacagc
     4741 ggcaagcaga atacgcccct cctgctcctc aggctcctca gcaacaagta cagtacaccc
     4801 ccccggctcc gccgatacac ggtgaccagc agctgttgca caattccatt tcaccaacct
     4861 ccggcgttcc agttcgtgta gtgcagccag gcacgcctcc caatctctct cccggcttat
     4921 tgacggagag tccttcacca gtgctcccct cctctccatt atcaccggag agtgaggcgc
     4981 cactgtatat tcccccggct ccgtatagcc ctgagagtat tatcgcatcg cctgatattc
     5041 tcacagaagg aggcaaaccg gtatatactc ctccgcctcc gggagcggaa cttcctcagt
     5101 aactagcaat ctgtttcatt tcattctata atattgtcgg acggaaaaat gtaccaagag
     5161 aacactttgc atctctagaa cataacgact cagaaaaagc gctttgcgtg tttttgctcc
     5221 tgagaaaaat cccaaaggag gatggtttac catgagaaga agaatactca tcgtactcag
     5281 tttaacagtc ctgctctctc tcctaccatc atctacaaca gcgacaacat acgttgtcga
     5341 accggttctc gtggcgcaac ccgtgtattc aggaatgtcc ttttatgtct accggccata
     5401 taatatgccc tccggctggt ttgtcacgtt cgacgggtat ccaataacac gcacccctgc
     5461 gggagtctgg gtttatggtt catataacgg atcccttctt gctccaacgt cgtttgtagt
     5521 cggttctatc tctccccaat cacttcctat tgtccagtat gtaccttcag cacgtgtttc
     5581 cagcacacag agcctaccgg ttacgccgct tccaccgctc cccgccccaa tccaacctga
     5641 tccgggggct tatattttgg ctgaagccgt gacagtcgct ccgacatatg tcccggagtg
     5701 gacaacacaa tcaagtttta cttcaatagg cacttggaat aaactggtcg atagaatggg
     5761 aattcttgag aaaccaagag ttccgatcgc atggaaggga gaacgtcctg aagccctttt
     5821 cgtctggacg ggaaagagct ggtaccagat tcaagcccgc tccggcgttt cagaaaaacc
     5881 atcagaaatg ataaaagatc atctttatgc gcttactcga atggtgaaga acaacgcata
     5941 ccactggaaa gacgcagata cggcaattct tgccaatcag gccgcagtct ggggatatct
     6001 ttggatggga agaatcgctc cttcaaacct atagataatt gttcctcgtt caaaagacac
     6061 ttctcgttag tttgagataa aacaaggggg caatcagatg aaaaaatctt tcaggtatat
     6121 ttttttcttg ttctcttaca cattcacgtt tctttttttc tttccgggtg cgtcttctgc
     6181 gaattatgtc ctcattcctg cgcaagcacc ggcaattatt acaactcctg actatgtcga
     6241 ttcttttcaa gctccgtccc cctctgctct atctggacaa attttccgct caaaggaagg
     6301 atatcttttc tcatttgacg cctcaggacg ccgcgtttat cacatgcacg cttcgtatgt
     6361 tctttccgct catggcagca gcccggttcc ggtacatatt attgctgccc cgatgattac
     6421 agtacatccg ggcgctgtgc tcactccatc cccctataca tatcttccgc aacaatatct
     6481 gcttgtaccg taaatctcgt ttttctctct gacatcatca tgacgaagag ccgcccttga
     6541 ggaaatcctc actgggcggc tcttcgtcat gaaagtcccg gagagcgtcg ttgtcgtata
     6601 ctc
//
LOCUS       DAOSWW010000027         6580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_7642_length_6580_cov_3.740536, whole genome shotgun sequence.
ACCESSION   DAOSWW010000027 DAOSWW010000000
VERSION     DAOSWW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7642_length_6580_cov_3.740536"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK442_01115"
     CDS             complement(<1..206)
                     /locus_tag="PK442_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOO86275.1"
                     /translation="MFDALRERFEAVFDKLKGRGKLTETDVETALRDVRRGLLEADVD
                     YKVVKSLLEAIKTRAVGRDVLESIT"
     gene            complement(209..553)
                     /locus_tag="PK442_01120"
     CDS             complement(209..553)
                     /locus_tag="PK442_01120"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOO86276.1"
                     /translation="MRTTKEETVLAKRVYLHDLYDLYAPLLTEKQREAWDLHEFADFS
                     LAETAEKLGISRQGVSDLITRSRDRLEELESLLGFFHKEDAFEEEIRELRRRIEHLSS
                     GRGEAMQKAEGN"
     gene            671..1372
                     /locus_tag="PK442_01125"
     CDS             671..1372
                     /locus_tag="PK442_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOO86277.1"
                     /translation="MDENSKRTFPLVEAMKLTNQFDTARQEHRDRLGGTLVKRLEERG
                     FHALYVQTKEEALKEVVKLIPEGASVGVPGSVTIREIGAMEDLAKRGCTVIHHWNPSL
                     SAEERMDTLQNELLADYFLTSSNAITLDGLLVNIDGNGNRVSGMAWGKNTLIFVIGLN
                     KVTNSLDEAIARTRNQATPPNALRLKLQTPCASTGRCSDCNSPDRACKALLVLERATG
                     GRTSHVILVGEDLGF"
     gene            1464..2459
                     /locus_tag="PK442_01130"
     CDS             1464..2459
                     /locus_tag="PK442_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOO86278.1"
                     /translation="MSSRGKIALVIAGGQIGMRFNEQMNSWQPVITPEEMLEWLPGET
                     AGKIFTVDWSHQPSSHYSVRMTADLVQVLSKTVVDGADGIVVTCGTDTLEEMAYLADL
                     YWAYPQPLVFTASIYPYDMPGSDAHLNLTQAVQAAFSKECWGLGVLVCVQEQLFAASE
                     ITEIASQRQDAFAAPGRGPLAQFIGDKVDIIRQYKRSKVLEGSMTPARDVELLSVALG
                     GGERLLEILSSDEKRELDGLVIAGFGNGHVPPSWIPRIKKLLKDDVPVVITTRCPEGY
                     TREIPYSFEGSMSRLLEMGVLQGGSLRPAQARLKLAAGLGAGLRRSDLQQYLLEK"
     gene            2622..3047
                     /locus_tag="PK442_01135"
     CDS             2622..3047
                     /locus_tag="PK442_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOO86279.1"
                     /translation="MKYAATDEIIAIRLSEGEDLADSLAHVCKACDVDSAVIVSAAGM
                     VSSVTFGWYNGRDYNTESVREIMELTSLSGNVSFRGGSLYPHLHGVFNKPDHAALSGH
                     LLQAIVFNNAELFLKPLATVHLGRSFDGSFEALSPEKKM"
     gene            complement(3107..3628)
                     /locus_tag="PK442_01140"
     CDS             complement(3107..3628)
                     /locus_tag="PK442_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOO86280.1"
                     /translation="MDSVIQHAQEETFVIEESSSDPVEKVLRFLNERGFHGAVTYTEE
                     TIFTVEDASRVVGAQPEHILKSLVLLVDDRPALALMSGTNRVDVKKVRREAEAKKVRM
                     ADPEYVYEWSGFKIGGVPPVGYPELPEAFLDEDLFLYPVVWAAAGTDHSFFPIAPGDL
                     LRLAEGRKCSIKK"
     gene            complement(3632..4513)
                     /locus_tag="PK442_01145"
     CDS             complement(3632..4513)
                     /locus_tag="PK442_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO86281.1"
                     /translation="MDFRELQSLVEVMEKGSISAAAASLGISQPAVSKHIAKLEREMG
                     VKVFARGQKCSNLTVEGEVLYKFARKTISQLADIKRDFSDIADDVSGIVRISASSIPG
                     DYILPGILVEFRKLYPNIEVEVRISDSREAVEKLVTRESDISVTGHGRHASGFNTVPF
                     ARDELVLLVAPGHPLAERENITLRDLENMDLVGRVTGSSTSRIWEDAFKAHTGKGKHV
                     YLKFGHVNAVVEAVRNGAEGAVISRLAGEDVSGLVMIPFRPALMRTFSITYGTMTTKA
                     METLLEFLLERKTFKDE"
     gene            4704..5075
                     /locus_tag="PK442_01150"
     CDS             4704..5075
                     /locus_tag="PK442_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO86282.1"
                     /translation="MSLGLRIRTLRKALGLTQQGLAERTQVSRIYIQALESNRRTPSM
                     KLLSRLANSLEVEVQDLVKTMSTSPSGRLQLEEVFQAAPETEVWYRSKRLKPKELRFV
                     QSLIDAAIERWEEEDRADGSV"
     gene            5102..5533
                     /locus_tag="PK442_01155"
     CDS             5102..5533
                     /locus_tag="PK442_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOO86283.1"
                     /translation="MGSRKQSQSTEFTFPSPPDFLPEWALREGGDWAGLDEFSLLVKA
                     EERTGKILELEYHDLPFGTWGVHIVRGRRGLIFINKRLSPYWRRFALFHELYHFLEHN
                     GGMELWTRTATPLSSFEHQADLFAWGALRKEWGRCWAHAFP"
     gene            5509..6501
                     /locus_tag="PK442_01160"
     CDS             5509..6501
                     /locus_tag="PK442_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO86284.1"
                     /translation="MLGSRISMSAALPKIPFFLPMDSCPRRCVYCHQGEITGISEVPS
                     PDAVQQTLSRLQAPHEVCYFGGSFTCFPEERRRAYMEAVFAAPERSCVRFSTHPHCLS
                     PGILDSLASYPVSMIELGISSLDDDVLNACNRGYTGEFAIAAMRLILERGFSLGAQLM
                     IGLPGQRRADLARDIELFRELDLDMIGVGPFVPDPQTPLGQGGLRAELGDDQVANDAT
                     TTYTVIALARLACPRANIPSTTALATREGADGQMLALSCGANVIMPNVTPARYRTQYE
                     IYPQKACYYDDSQAAATSLRKRIAALGRTVGIGRGDSPNVQRRDPEKHETVGRK"
BASE COUNT         1594 a         1724 c         1729 g         1533 t
ORIGIN      
        1 gttatggatt caagaacatc tcttcctacg gcgcgggttt tgatggcctc cagaagggac
       61 tttacgactt tataatcgac atcggcttcc aaaagcccgc ggcgaacatc cctcagggca
      121 gtttcaacat ctgtctcggt gagtttaccg cgtcctttca gcttgtcgaa cacggcctca
      181 aaacgttcac gaagcgcatc gaacatattc agtttccctc cgctttctgc atcgcctcgc
      241 cccggcccga agaaagatgc tcaatacggc gccgaagctc gcggatttct tcttcaaacg
      301 cgtcttcttt gtgaaaaaaa ccaagaaggc tttccagctc ctcaagtcga tcacgacttc
      361 gcgtaataag atcggacacg ccctgacggc tgattccaag tttttcggct gtctcggcaa
      421 gagagaaatc cgcaaactca tggagatccc aagcctcacg ttgcttttca gttaacaatg
      481 gggcataaag gtcgtacaag tcgtggagat acactctctt ggctaaaacc gtttcttctt
      541 tcgtcgtgcg catctcgcag cctcccctgg aactgtggaa ttatatcccc cttcgcaaaa
      601 agtgtcaagc tttttctctt gacagcattc tatattccgg agcgaaacgt tcggagagaa
      661 aaatgatatg atggacgaaa attcaaagcg gacattcccg cttgtggagg cgatgaaatt
      721 gacgaatcag ttcgatacgg cccgacagga acaccgcgac cggctgggcg gtacattggt
      781 aaaaaggctg gaagaaagag gctttcacgc tctctatgtt cagacaaaag aagaggccct
      841 caaagaggtt gtaaagctca tcccggaggg cgcctccgtc ggcgtccccg gcagcgtcac
      901 aattcgtgaa atcggcgcca tggaagacct tgcgaagagg ggatgcaccg taatccatca
      961 ctggaacccg tccctgtctg cggaagaacg catggacacg cttcaaaacg agctcttggc
     1021 cgattacttc cttacaagct cgaatgccat cactctcgac ggattgctgg tcaatatcga
     1081 cggaaacgga aacagggtaa gcggcatggc gtgggggaaa aacacgctta tcttcgttat
     1141 tggactgaac aaagtgacga acagtttgga cgaggcgatt gcacgaacgc gcaaccaggc
     1201 tactcccccg aatgctcttc ggctaaaatt gcagacgccg tgcgccagca caggacgttg
     1261 ctcggactgc aactctcccg acagggcctg taaagcgctc ctcgttcttg agcgcgcaac
     1321 cggcgggaga acgtcgcatg tcatccttgt cggagaggac ctgggctttt aaaaccgaac
     1381 aaatgccata aagggtgtgc ttttttcggg cttttagtgt acacttgccc cgtcacggtc
     1441 agatggggaa ggaggacctt tttatgtcca gtagaggaaa gatcgccctt gttatcgcag
     1501 gcggccagat aggaatgagg ttcaacgaac agatgaattc ctggcagcct gtaatcaccc
     1561 cagaagaaat gctcgaatgg ttgccgggag agacggcggg taaaattttt accgtcgatt
     1621 ggagtcatca acccagcagc cattacagtg taagaatgac tgcggatctt gtacaggtac
     1681 tttctaaaac cgtagtggac ggcgctgatg gtattgttgt aacgtgcggc accgacaccc
     1741 tggaggagat ggcatacctc gccgatttgt actgggcgta ccctcagccg ttggttttca
     1801 ccgcgtcgat ttatccgtat gacatgccgg gctccgacgc tcatctcaac ctgacgcagg
     1861 cagttcaggc cgctttttcc aaggagtgtt ggggattagg cgttttggtc tgcgtgcagg
     1921 aacagctttt cgcagcgtcc gaaataacgg agatcgcgag ccaaagacaa gatgcctttg
     1981 ccgcacccgg aagaggcccg cttgctcagt ttatcggcga caaggtggat attatcaggc
     2041 agtataaacg atctaaagtt cttgaggggt ccatgacgcc ggcgcgtgat gtcgaacttc
     2101 tttccgtcgc ccttgggggc ggtgaaaggc tccttgaaat tctttcatcc gacgagaaac
     2161 gagaactcga cggtcttgtt attgcaggtt tcggtaacgg ccatgtgcct ccttcatgga
     2221 ttccgcgaat aaaaaaactt ctaaaagatg acgtgcctgt agtgataaca acacgatgcc
     2281 cggaaggata cacgcgtgaa attccgtatt cttttgaggg aagtatgtcg cgcctcttgg
     2341 agatgggggt ccttcaaggc ggttctttac gtccggcaca ggcaaggctc aagcttgccg
     2401 ccggactcgg cgcgggactc cgtcgatcgg atctgcagca ataccttctt gagaaataaa
     2461 gcaaaaagac aacttcgttt tttagccgca ttcggttgaa agaggcattc ttctgagaag
     2521 tggcggagag aatcaataca tggtagaata ttgaaggaat acaagagaac cgctttccgc
     2581 cgggcagcgc acgccggaaa tatgaaagga ggacgagaaa catgaaatat gccgctaccg
     2641 atgaaattat cgcgatacgt ctttcggaag gagaagatct cgcagattct ctggcgcatg
     2701 tctgcaaggc atgcgacgtc gattcggcag ttatcgtctc cgcagcggga atggtgtctt
     2761 ccgtaacttt cgggtggtac aacggacgtg attataatac ggaatcagtg cgtgaaataa
     2821 tggaacttac ctcgctcagc ggcaatgtaa gcttccgcgg aggcagtctc tacccgcatc
     2881 ttcacggcgt tttcaacaaa cctgatcatg ccgcgctttc cggacatctt ctccaggcca
     2941 tcgtattcaa caacgcggaa ctttttttaa aaccgcttgc aacggttcat cttggacgct
     3001 ctttcgacgg ctcttttgaa gccctttccc ctgagaaaaa gatgtaacct cgcacacagt
     3061 gcgacccaaa aagttcctcc cgaaaacggg aggaactttt tttcgcttac tttttgatgc
     3121 tgcacttcct gccctctgcg aggcgcaaca aatcaccggg agctatggga aaaaacgaat
     3181 gatccgttcc tgcggcagcc cagacaacgg ggtacaaaaa gagatcttca tcaagaaacg
     3241 cctcgggcaa ctccggatat cccacaggag gcacgcctcc tattttgaaa ccggaccact
     3301 cgtaaacata ctccggatct gccattcgaa cttttttcgc ttccgcttct ctgcgtactt
     3361 tcttaacatc cacgcggttc gttccggaca taagagcaag cgccggccga tcgtctacaa
     3421 gaagcaccaa gcttttcaaa atatgctccg gttgggcgcc gacgaccctg gatgcgtctt
     3481 ccacagtaaa gattgtttct tccgtgtatg tgaccgctcc atgaaaacct ctctcattca
     3541 aaaagcggag gaccttttcc accgggtcgg aagacgactc ttcgataacg aaggtctcct
     3601 cttgtgcatg ctgaattaca gagtccactt tctactcgtc cttgaaggtt tttctctcaa
     3661 gcaaaaactc gagaagcgtc tccatcgcct ttgtcgtcat ggttccgtac gtgatcgaaa
     3721 atgtccgcat aagggcggga cggaacggta tcattacaag cccggagacg tcctcgcccg
     3781 caagacggga aatgactgct ccctcggccc cgtttcgtac agcttcaaca accgcattga
     3841 cgtgaccgaa tttgagatag acatgcttcc cttttccggt atgggctttg aatgcatcct
     3901 cccagatacg cgatgtcgac gaaccggtga ctcttcccac aaggtccatg ttctccagat
     3961 cgcgcagagt gatgttttcg cgctccgcaa gcgggtgtcc gggagcgact agaagaacca
     4021 gttcatctct ggcgaaagga accgtattga atccggatgc atgtctcccg tgtcccgtaa
     4081 cggagatatc ggactcccgg gtaacaagtt tctcaacggc ttctcgcgaa tctgaaatgc
     4141 gcacctccac ttcgatattt ggatagagtt tccggaactc tacaagaata ccgggaagaa
     4201 tataatcgcc cggtatggag cttgcgctta tcctcacaat acccgataca tcatcggcta
     4261 tgtccgaaaa gtcgcgtttg atatccgcaa gctgagagat agttttacgg gcaaatttat
     4321 acaacacttc cccttctact gtcaggttgg aacatttctg tccccgggca aatactttga
     4381 cccccatctc tctttcaagc ttcgcgatgt gtttgctgac tgccggctga gaaattccga
     4441 gtgatgctgc cgctgccgag atgctccctt tttccataac ttctaccaga ctttgcagtt
     4501 cacgaaaatc caaagcggat acctccccaa agacaatata ttctttttcc atccaaacgc
     4561 cccgacataa atatattctg aaagagaaag aacttcaaga gccaaagata tttttcttca
     4621 aaagaaggca agggcttgaa cttaatgtaa tcaagtatat aataatatat atccaaagaa
     4681 aacaagacgg aggagggatc gcgatgagcc ttggactgag gatacgcact cttcgtaagg
     4741 ccttgggcct gacacagcag gggcttgcgg agagaacaca ggtgagcagg atatacatac
     4801 aggcattgga aagcaaccgg agaactcctt cgatgaaact cctctcgcgc cttgcgaatt
     4861 cacttgaggt ggaagtacag gatctggtaa agacaatgtc gacatcgcct tcagggagat
     4921 tgcaacttga agaagtcttt caagccgctc ccgagaccga ggtctggtac agaagcaaga
     4981 gactgaaacc caaggaactg cgtttcgtcc agagcctcat cgacgcggca atcgaacgtt
     5041 gggaagaaga ggaccgcgcc gatggaagcg tctgatcctt cttcacgccg gattttctgt
     5101 tatgggcagt cggaaacagt cccaaagcac tgaattcacg ttcccttcgc cccctgactt
     5161 tttacccgag tgggcgttgc gagaaggcgg cgattgggcc ggtctcgatg agttctcgct
     5221 cctcgtaaaa gccgaagaac ggacgggaaa gattcttgag ctcgaatacc acgatctgcc
     5281 atttggtaca tggggcgtac acatagttcg cggacgaagg gggctaattt tcatcaataa
     5341 acggttgtcg ccttactgga ggcgcttcgc tctttttcat gaattgtacc attttctgga
     5401 gcacaacggg ggaatggagc tgtggacccg taccgctacg ccgctctcga gtttcgagca
     5461 tcaagcggat ctcttcgcat ggggcgcgct gcgcaaggaa tggggcagat gctgggctca
     5521 cgcatttcca tgagcgccgc tctcccgaaa attccatttt ttcttccaat ggattcctgc
     5581 ccgaggcgtt gtgtctactg ccaccaggga gagatcacag gaatctccga agttccttct
     5641 ccggacgcag tacagcaaac cctctcccgg cttcaagctc cccatgaggt ttgttatttc
     5701 ggaggtagct ttacctgttt cccggaagag cgcaggcgcg cgtacatgga agccgtcttc
     5761 gccgctccgg agaggagctg cgttcgtttc tcaacacatc cgcactgtct ctcgcccggt
     5821 attctcgaca gtcttgcttc ctaccccgtt tcaatgatcg aactcgggat ctcctcgctc
     5881 gacgacgatg tgctgaacgc atgtaaccgg gggtacacag gagagttcgc gattgcagcg
     5941 atgcggctta ttttggaaag agggttttct ctcggggcgc agttgatgat cggtcttccg
     6001 gggcagcgcc gcgcagacct ggcccgcgat atcgagttgt ttcgcgagtt ggatctggac
     6061 atgatcggcg ttgggccgtt tgtgcccgac ccgcaaacgc cgctggggca gggcggttta
     6121 cgtgcggaac ttggtgacga ccaagtcgcg aacgatgcca cgacgacata cacggtgatc
     6181 gcgctcgcac ggttggcgtg cccgcgggca aatatcccga gcacgacggc cttggcgacg
     6241 cgggaaggtg cggatgggca gatgctcgcg ctctcgtgcg gggcgaatgt gattatgccg
     6301 aacgtgacgc ctgcccggta tcgcacgcaa tatgagattt atccgcagaa ggcgtgttat
     6361 tacgacgatt cgcaagccgc cgcgacgagt ttgcgtaaac gaatcgcggc actcgggcgg
     6421 acggtaggta ttggtcgcgg cgattcgccg aatgtgcagc ggcgcgaccc ggagaagcac
     6481 gaaacggtag gtaggaaatg agtgtgaagt gccatacgtc gcccgatcgg acgctgagcg
     6541 aaggggcgac gaatttcatt gatgcggcga agttgagcga
//
LOCUS       DAOSWW010000028         6558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_7679_length_6558_cov_3.451023, whole genome shotgun sequence.
ACCESSION   DAOSWW010000028 DAOSWW010000000
VERSION     DAOSWW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7679_length_6558_cov_3.451023"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="PK442_01165"
     CDS             <1..1780
                     /locus_tag="PK442_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86285.1"
                     /translation="QEEAYTTPFFARKIIHPDFEQFFESAWEDLQRLEAPPTFEFKVL
                     AKSGEEFWVYQRNVLVCGDDGEPVALEGLVTDISDRKAAEAALDNYREHLEELVRDRT
                     AELRIAKEAAEQANRTKSAFLANMSHEIRTPLNAVLGFASILERDGGLSEEQADQVRT
                     INRSGRHLLKLINDILDMSRIESGQLELSVTEFCLRDLLDDMETMFRSRTDAKGLHFV
                     MERMESVPRYAAGDEAKLRQILFNLLGNAVKFTKTGGIAVRARADKEHDAEDTVRFLV
                     EIEDTGPGIPEKDLEHIFDAFRQSEAGRDAGGTGLGLSISKRLAELMGGSLTVQSKIG
                     TGTCFTLSVPLHTATSGHRTAKMDRQDIVGLDTGDAFFRILIVDDVKENRDVLRAMLS
                     PDRNAGSATMFRGRTSAFELREAVNGEEALSIFEEWFPHVVLMDMRMPVMDGYEATRR
                     IKAVPGRGSVPVIAITASAFEDDRDAVLKAGVDRYIRKPLRAEELFETLGDVLGLPVL
                     RGGGSAKASGSDETLPSEEEMKVLPGEVLAAMHEAVENGDIAGLDELIVKTREIDAGI
                     AEKLRTLARRYDYETLSRLLKNGERG"
     gene            1823..4159
                     /locus_tag="PK442_01170"
     CDS             1823..4159
                     /locus_tag="PK442_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO86286.1"
                     /translation="MTQPTTYRTPAFFRRIPMLLAASLFLAALLFSSPLCGESLTVKV
                     ALHSGKPGIFIDESGTPDGIFVDILEHIAGIEGWKLEYVPGTWAEGLERLKQGEVDVV
                     TSIALTSEREKIYTFTGTPALSAWSAAYVARGSGIVSLLDLKGKRVAVMKNTVQESAF
                     IKHIQGFDLDIEILSVPDQDAAFSMVADGTADAAIEVSTTGEVYRQRYELENSAIVFN
                     AVQAYYAAAREDPKGLASAINRHLYAMKADPSSPYYEIMKRWFPKKTGRSAVERRLWI
                     ASGVFLAGLLLSLSMLAALRWQVQRRTFELALAKEEAEKANKAKSSFLANMSHEIRTP
                     LNAILGFGAVLERDPSLSPKQAEYVNAVSRSGRHLLKLINDILDMSRIEADRLVLNPT
                     DFNLRDLLADMRMMFGARAEEKHLQLIMEIQETLPVYARGDEAKVRQIFVNLLGNAVK
                     FTLSGGVAVRARMDKPREAESASFLLVEVEDTGPGIPPEDMKKIFDPFRQSDAGKQAG
                     GSGLGLSISKRLAELMGGALTASSTVGKGTCFRLRLPLHTAEHTAEHPRTALRSIVGA
                     ESASSYRILLVDDIKENRDVLRAVLEPLGFSLMEAVDGAEGVKLFEEWTPHAVLMDMR
                     MPVMDGYEATRRIKETPEGAAVPVIAITASAFEDEVQNVLNSGADYCIRKPLRPEELF
                     EILGEALGLRYVYGADTAEHEEKPHEITAGDVARLPESLTSAMRTAVEEGDIFQLEAL
                     INTIQEVDAPLADGLRRLARRYDYETLTDLLPEEVESP"
     gene            4156..5265
                     /locus_tag="PK442_01175"
     CDS             4156..5265
                     /locus_tag="PK442_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HOO86287.1"
                     /translation="MNDHNEQTTIMLVDDTPANLELLRAMLHKKGYKVLVFPGGEPAL
                     RALERKEPDLILLDIMMPEMNGFEVCGRLKEDERLREIPVIFISALDDTQSKVRAFTA
                     GGVDYVTKPFQPEEILARVQTHVTLRQVRRELEKHMSELEELVREKVKEISDSQMATI
                     HAMSKLAEYRDDQTGRHIERTRELCAMLAGKLRENPRYALLIDDAFVENIYHASPLHD
                     IGKVGIPDAILLKPGKLTEEEFEIIKRHPRIGATTLSWVRSKYPQNAFINMGIALALF
                     HHERWDGGGYPDGLAGEDIPLSARIMALVDVYDALRSDRPYKEAFPHETACRIIREGN
                     GTHFDPSVVDAFFAVEQEFEQICDHMTNEGAEGIL"
     gene            5302..>6558
                     /locus_tag="PK442_01180"
     CDS             5302..>6558
                     /locus_tag="PK442_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO86288.1"
                     /translation="MKTTTRTRGSLPTGQTAEKGFHSSWNGSRYREFLLLIAPLLLFS
                     AYPLLRSFEHAGNSGVFGGVRMTGALIALSAGCAFIARFAGPGRRFYLFAGLAFCVAG
                     AEDLAAGFLELAGASFAQAPREAVLRSSIAFRTLTPLLLMAPLVISAGKGVPRRPGRE
                     LLPALLSVPLIIALTLYAALNGSPLPFHTLLSRPLDFLSAALFAAALIVYVREFRSSG
                     ESIAWWLGLSIGVMLAGQSLLLFSRGIYDPFFAAARVCAILGYAVSLLGFSLNQLSVE
                     DDQNWTIFRMSAEVDRINSIYLEDVVLEKIREVADSHMATILAMSKLAESRDDETGRH
                     IERTQELCRVLARKMRELPRYASVIDDAFIENIYHAASLHDIGKVGIPDAVLLKEGAL
                     SEEEREMIRRHPGIGAATLERVRREHP"
BASE COUNT         1359 a         2047 c         2058 g         1094 t
ORIGIN      
        1 ccaggaggaa gcatatacca cgcccttctt cgccaggaag atcatccacc ccgatttcga
       61 acagtttttc gaaagcgcgt gggaggatct tcagcgcctt gaggcgcctc cgacgttcga
      121 attcaaggtg ctcgccaaat cgggagagga gttctgggtg taccagcgca atgtgctcgt
      181 ctgcggcgac gacggagaac cggtcgccct cgaaggcctc gtcaccgaca tcagcgacag
      241 gaaagcggcg gaggccgccc tggacaacta ccgggagcac ctggaagagc tggtccggga
      301 ccgcaccgcc gaacttcgga ttgcaaaaga ggcggcggaa caggccaacc gcaccaagag
      361 cgccttcctc gccaacatga gccacgagat ccgcacgccg ctcaacgcgg tcctcggatt
      421 cgcctccatt ctcgaacgcg acggcggtct ctccgaagag caggccgatc aggtgcgcac
      481 catcaaccgg agcgggcggc acctgctgaa actgatcaac gacattctcg acatgtccag
      541 aatagagtcg ggacagcttg agctctcggt gacggagttc tgcctccggg acctgctgga
      601 cgacatggag acgatgttcc gctcccggac cgacgccaag ggactgcact tcgtgatgga
      661 gcgcatggag agcgttcccc gctacgcggc gggggacgag gcgaagctgc ggcagatact
      721 cttcaacctg ctcgggaacg ccgtcaagtt caccaaaacc ggcggcatcg ccgtacgcgc
      781 ccgtgcggac aaggagcacg acgctgagga taccgtgcgc ttcctcgtgg agatagagga
      841 tacgggaccg ggcattccgg agaaggattt ggagcacatc ttcgacgcat tccgccagtc
      901 cgaggccgga cgcgacgcgg gagggacggg actcggcctt tcgatcagca agcggctcgc
      961 cgagctgatg ggcggctccc tcaccgtaca aagcaaaatc gggacgggaa cgtgtttcac
     1021 cctctccgtt cctctgcaca cggcaacgtc gggacatcgt acagcgaaaa tggaccggca
     1081 ggacatcgtc ggcctggaca cgggagacgc cttcttcaga atactgatcg tcgacgacgt
     1141 caaggagaac cgcgacgtgc tgcgcgcgat gctctctccc gaccggaacg ccggttccgc
     1201 gacgatgttc cgtgggagga cttccgcctt cgagctgcgg gaagcagtca acggtgaaga
     1261 ggcgctgtcg atcttcgaag agtggttccc tcacgtcgtg ctgatggata tgcgcatgcc
     1321 ggtcatggac ggctacgaag ccacgcggcg gatcaaggcg gttccgggaa ggggctcggt
     1381 ccccgtcata gcgatcacgg cgagcgcctt tgaagacgac agggacgctg tgctgaaggc
     1441 cggggtggac agatacatac gaaaaccgct cagggcggag gagctgttcg agacgctggg
     1501 agacgttctt ggcctgccgg tcctccgcgg gggcggttcc gcaaaggcgt ccggaagcga
     1561 tgaaacgctc ccgagcgaag aggagatgaa ggtccttccc ggagaggtgc ttgcagcgat
     1621 gcacgaggcg gtagagaacg gagacatcgc gggtctcgac gagcttatcg taaaaacgag
     1681 ggagatcgac gccgggatcg ccgaaaaact caggacgctc gccaggcggt atgattatga
     1741 aacgctctcc cgcctgctga agaacgggga gagaggctga gcatcccatg ccggcctccc
     1801 gaaaatgaag gagatgaaaa cgatgaccca gccgacgaca taccgaactc cggcgttctt
     1861 ccgccgcata ccgatgctgc ttgccgcctc gctgtttttg gcagcgcttc tcttttcttc
     1921 ccccctttgc ggagagagcc ttacggtcaa agtcgccctg cattcgggca aacccggcat
     1981 cttcatcgac gaatccggaa cccctgacgg catcttcgtc gacatcctcg aacatatcgc
     2041 cggaatcgaa ggctggaagc tggagtacgt ccccggcaca tgggcggagg ggctggagcg
     2101 tctgaaacag ggggaagtcg acgttgttac aagcatcgcc ctcacctccg aacgggagaa
     2161 gatctacacg ttcacaggga ctccggcgct ctccgcatgg tccgcggcgt acgtcgccag
     2221 agggagcggc atcgtctccc tgctcgacct gaaggggaag cgcgtcgcgg tgatgaagaa
     2281 cactgtgcag gagtcggcct tcatcaaaca catccagggc ttcgacctcg atatcgagat
     2341 cctctccgtt ccggatcagg acgcggcgtt ctccatggtc gccgacggaa ccgccgacgc
     2401 cgcaatagag gtcagcacga cgggcgaagt ctacagacag cgctacgaac tggagaacag
     2461 cgccatcgtc ttcaacgcgg tacaggccta ttacgccgcc gcccgggaag acccgaaagg
     2521 ccttgcatcg gccatcaacc ggcatctgta cgcgatgaaa gcggatccct cctcgcccta
     2581 ctacgagatc atgaagcgct ggttcccgaa aaagaccggc agaagcgcgg tggaacgccg
     2641 cctctggatc gcctcgggcg tttttctggc ggggttgctt ctcagtctgt caatgctggc
     2701 ggcgctccgc tggcaggtac agcgacgcac cttcgaactt gccctggcca aagaggaggc
     2761 ggagaaggcc aacaaggcca agagctcctt cctcgccaat atgagccacg agatccgcac
     2821 gccgctcaac gccatcctcg gcttcggcgc ggtgctcgaa cgggatccgt cgctctctcc
     2881 aaaacaggcc gagtacgtga acgccgtcag ccgaagcgga cggcatcttc tgaagctgat
     2941 caacgatatc ctcgacatgt cgaggatcga agccgaccgg ctcgtgctca acccgaccga
     3001 cttcaacctt cgcgatctcc tggccgacat gcggatgatg ttcggcgccc gcgccgagga
     3061 aaaacatttg cagctcatca tggagattca ggagactctt ccagtgtacg cgaggggcga
     3121 cgaggccaag gtacggcaga ttttcgtcaa cctcctcgga aacgccgtga aattcacgct
     3181 ctccggcggc gtcgcggtac gcgccagaat ggacaagccg cgcgaagcgg aaagcgcttc
     3241 cttcctcctc gtcgaggtcg aggacaccgg accgggcatt ccccctgagg acatgaagaa
     3301 gatcttcgac cccttccgac aatcggacgc cggaaaacag gcgggaggtt cgggactggg
     3361 actgtccatc agtaaacggc ttgcggagct tatgggcggc gcgctcaccg cctcgagcac
     3421 cgttggaaaa ggaacgtgct tccgcctgcg ccttcctttg cacacggcgg aacacacggc
     3481 ggaacatccg cggacagccc ttcgcagcat cgtcggcgct gaatccgcct cttcctaccg
     3541 gatattgctg gtcgacgaca tcaaagaaaa cagggacgta ctgcgcgccg ttctcgaacc
     3601 cctgggcttc agcctgatgg aagcggtcga cggggccgag ggggtgaaac tcttcgaaga
     3661 atggacgccg cacgccgtgc tgatggatat gcgcatgccc gttatggacg gctacgaggc
     3721 gacgcgccgc atcaaggaga cccctgaggg cgccgcagtc ccggtcatcg caatcacagc
     3781 gagcgcgttt gaagacgagg tgcagaatgt cctgaacagc ggagccgact actgcatacg
     3841 caaacctctc cgaccggaag agctgttcga gatactcggc gaggccctcg gactgcgcta
     3901 cgtctacgga gccgacaccg ccgaacacga agagaagccc catgaaatca cggccgggga
     3961 cgtggcgcgg ctgcccgaat cgctgacgtc cgccatgaga accgccgtcg aggaggggga
     4021 catcttccag ctggaagcgt tgatcaacac gattcaggaa gtcgacgcgc cgcttgcgga
     4081 cggactgcgg cgtcttgccc ggagatacga ctacgaaacg ctcacagatc ttttaccgga
     4141 ggaggtggaa tccccgtgaa cgatcacaac gaacagacga ccatcatgct ggtcgacgat
     4201 accccggcca acctcgaact tcttcgggcg atgctgcaca aaaaagggta caaggtgctg
     4261 gtcttccccg gcggagaacc ggcgctccgc gcgctcgaaa ggaaagaacc cgatctgatt
     4321 ctgctcgaca tcatgatgcc ggagatgaac ggttttgaag tatgcggccg gctgaaagag
     4381 gatgaacgcc tccgggaaat tcccgtgatc ttcatcagcg cgctggacga cacgcaaagc
     4441 aaggtgaggg cctttaccgc cggaggcgtg gactacgtga ccaaaccgtt tcagccggag
     4501 gagatcctgg cgagagtgca gacgcacgtt acgctgcggc aggtgcggcg cgagcttgaa
     4561 aaacacatgt cggaactcga agagcttgtc agggagaagg tgaaggagat ctccgactcg
     4621 cagatggcga cgatccatgc gatgtcgaag ctggcagagt accgggacga ccagaccggg
     4681 cggcacatcg aacggacgcg ggaactctgc gccatgctcg ccggaaagct ccgcgagaac
     4741 cctcgctacg ccctcctcat cgacgacgcc ttcgtcgaga acatctacca cgcctcgccc
     4801 ctgcacgaca tcggcaaggt gggcatcccc gacgcgatcc tgctgaaacc gggaaagctc
     4861 accgaggagg agttcgagat catcaagcgg cacccgcgca tcggggcgac gacgctgagc
     4921 tgggtgcgca gcaagtaccc gcagaacgcg ttcatcaaca tgggcatcgc cctcgccctc
     4981 ttccaccacg agcggtggga cggggggggc tacccggacg gcctcgcggg cgaggacatt
     5041 cctctgagcg cgcgcatcat ggcgctcgtg gacgtctacg acgcgctccg ctccgaccgg
     5101 ccgtacaagg aggccttccc gcacgaaacg gcctgccgga tcatcaggga ggggaacggg
     5161 acgcacttcg acccttcggt cgtcgacgcc ttcttcgccg tcgagcagga attcgagcag
     5221 atctgcgacc acatgacgaa cgagggcgcg gagggcatac tgtaacgccg gccggccgtg
     5281 ccggggcgga ggggtgacat catgaaaaca acaacgcgaa cacgcggaag tctcccgacg
     5341 ggacagaccg cggaaaaggg attccactcc tcgtggaacg gaagccggta tcgcgaattc
     5401 ctgctgctca ttgcgcctct cctgctgttt tcggcgtatc cgctgcttcg ctcgttcgaa
     5461 catgccggaa attccggcgt gttcgggggc gtcaggatga cgggcgcgct gatcgccctg
     5521 tcggcgggct gcgccttcat cgccagattc gccggccccg gaaggcgatt ttacctgttc
     5581 gcaggcctcg ctttctgcgt cgctggagcg gaggacctcg cggccggctt ccttgagctg
     5641 gcgggggcat ccttcgcgca ggcgcctcgc gaagccgtcc tccggagttc gatcgccttc
     5701 cggacgctga cgccgttgct gttgatggcg ccgctcgtca tctccgccgg aaagggcgtg
     5761 ccccggcgcc ccgggcggga actcctgccc gcactgctct ccgtgccgct gatcatcgcg
     5821 ctcacgctgt acgcggcgct caacggctcg ccgctcccgt tccacacgct tctctcccgc
     5881 cctctggact ttctctccgc cgccctgttc gcagcggcgc tgatagtgta tgtccgcgag
     5941 ttccgaagtt cgggcgagag catcgcgtgg tggctcgggc tctccatcgg cgtcatgctg
     6001 gccggtcagt cgctgctgct cttctcgcgg gggatctacg acccgttctt cgccgccgca
     6061 agagtctgcg cgatcctcgg gtacgccgtc tcgctgctgg gtttctcgct caaccagctc
     6121 tcggtggagg acgaccagaa ctggacgatc ttccggatga gcgccgaggt ggaccggatc
     6181 aactccatct atctggagga cgtggtgctg gagaagatac gcgaagtcgc cgactcccac
     6241 atggcgacga tcctcgcaat gtcgaagctg gcggagtcga gggacgacga aacgggaaga
     6301 cacatcgagc gcacgcagga gctgtgccgc gttctggccc gcaagatgcg ggaactcccc
     6361 cgctacgcgt ccgtgatcga cgacgcgttc atcgagaata tctaccatgc ggcgtcgctg
     6421 cacgacatcg gcaaggtagg catccccgac gccgtcctcc tgaaagaggg cgcactctcc
     6481 gaagaagaga gggaaatgat acgacggcac cccggaatcg gggcggcgac gctggagcgg
     6541 gtacggcgcg agcacccc
//
LOCUS       DAOSWW010000029         6468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_7836_length_6468_cov_3.586465, whole genome shotgun sequence.
ACCESSION   DAOSWW010000029 DAOSWW010000000
VERSION     DAOSWW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7836_length_6468_cov_3.586465"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..739)
                     /gene="rnc"
                     /locus_tag="PK442_01185"
     CDS             complement(32..739)
                     /gene="rnc"
                     /locus_tag="PK442_01185"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOO86289.1"
                     /translation="MNFEDWYNGKLEKLQESIGYFFDDPTLLRMALLHSSFTHEHGGN
                     DHNERLEFLGDSVLQLSASHLLFISEKQLDEGALSRRRAALVCGNSLRRWAEFIDIPT
                     LLRTGKGMSGGTVRSSIYEDAAEALFGAVFLDGGYKAADAVILRYLDFLMFLSSPEPA
                     DPKTELQILAQERGIGIPSYEILSVTGPSHQPLFHVRVLLGGSPAGEGRGTSKKAAEF
                     NAAREGYTFFTKETATP"
     gene            complement(793..2031)
                     /gene="fabF"
                     /locus_tag="PK442_01190"
     CDS             complement(793..2031)
                     /gene="fabF"
                     /locus_tag="PK442_01190"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOO86290.1"
                     /translation="MRRVVITGLGVVSPIATGKEDYWQALKDGKNGIGPLTTFDASDS
                     PVPFGADIKDFDPSQWMDNKEAKRSDRVIQFAVAAAGLAVADAKLDTGSLDPYKFGVY
                     IGSGQGGIETTFNNFKTMMEKGPKRVSPFFIPMMISNMSTAYVAIKHNAKGPNMCVVT
                     ACATSVHSLGEAYHTILRDDADVILAGGTEAALRTISIAGFAAMKALSTRMDEPERAS
                     RPFDVDRDGFVMGEGAGVLVLEELEHAKRRRAHIYAELVGYGNTCDASHITAPDPEGD
                     GAARAMRKAMTHAKWLPEDVEYINAHGTSTPLNDKMEAIAIRSVLGDHTDKAYVNSTK
                     SMIGHCLGAAGALETVAALQTIEEGIIHPTINLEHLDPECAINVVGVSPVKAHVSRLL
                     VNSFGFGGHNGVIALQRFEE"
     gene            complement(2139..2390)
                     /gene="acpP"
                     /locus_tag="PK442_01195"
     CDS             complement(2139..2390)
                     /gene="acpP"
                     /locus_tag="PK442_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOO86291.1"
                     /translation="MKKEEMLARLKEIIIDRLDVEEDQIVAEASFVEDLGADSLDIVE
                     LIMGIEEEFDIEIPDEDAEKLTTVGEAMNYTLSKLGVDE"
     gene            complement(2418..3158)
                     /gene="fabG"
                     /locus_tag="PK442_01200"
     CDS             complement(2418..3158)
                     /gene="fabG"
                     /locus_tag="PK442_01200"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOO86292.1"
                     /translation="MEQRIALVTGAGKGIGRAVAIRLAKNGFFVAVNFRSSEKAALEV
                     CDIIAAGGGSAFPVKGDVSDPEEVKRVFSFVNEKAGNVSVLVNNAGITKDALLIRMKD
                     EDWNSVLTANLSSVYYCTREALRRMVRAKWGRVINIASVVGLIGNPGQANYCASKAGV
                     IGFTKSAAREYAAKGITVNALAPGFIGTDMTDALPQEARETLLKQVPAGRPGTPEDVA
                     NIACFLASDEASYITGQVIAVDGGMTMC"
     gene            complement(3160..4107)
                     /gene="fabD"
                     /locus_tag="PK442_01205"
     CDS             complement(3160..4107)
                     /gene="fabD"
                     /locus_tag="PK442_01205"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOO86293.1"
                     /translation="MSYSLIFPGQGSQEVGMGRDIRDAFKSAAEVFQRADDALSFSLS
                     KIIFDGPEEELRRTAYTQPAILVASIAAISVLEELVGKHLQPASLAGHSLGEYTALVA
                     ARTLSLEDGVRLVHLRGRLMQEAVPEGKGAMAAILGLDAQAVSDICQEVAPLHECQPA
                     NFNSPGQIVISGETEFVEKAMLLAKEKGAKKTVLLNVSAPFHSRLMRPVADQLLTAFK
                     QCAWGNPSAPIVSNVRALPVANIQDIQDALFHQTYMPVLWEKSVHWMADSGVDTFFEI
                     GPGTVLGALVKKCRKGLTAYPCGTLATLEKTAALLKEMM"
     gene            complement(4117..5091)
                     /gene="fabK"
                     /locus_tag="PK442_01210"
     CDS             complement(4117..5091)
                     /gene="fabK"
                     /locus_tag="PK442_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HOO86294.1"
                     /translation="MNHSTTSSHRRNRIVRLLGCSYPLIQGGMAWVADAELAAAVSNG
                     GGLGVIAAANMPPELLEQQLVKVRTLTDRPFGLNIMLMSPTVEDVIELAAKYSVPIVT
                     TGAGSPGKVIERLKPSGTIVVPVIASVAQAKRVEKQGADAVVAEGMEAGGHIGELTTM
                     VLVPQIVDSVEIPVVAAGGIADGRAVAAAFALGAEGVQVGTRFVCCTESTVHPSYKEA
                     ILAARDRSNVITGRITGHPVRCLKNKLTSRFEELEKENAPVEEYEKLGAGRLRAAVVE
                     GDVEWGSLMAGQSAALVNDILPAAEIIRNLFRDAEIISQGLSAALSLE"
     gene            complement(5081..6076)
                     /locus_tag="PK442_01215"
     CDS             complement(5081..6076)
                     /locus_tag="PK442_01215"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOO86295.1"
                     /translation="MSFFQGVPVAIRGSGMYAPRKVVTNNDLSQIVDTSDEWIRERTG
                     IQRRHIAEPGELTSTIAEKAGRAALENAGILPSELDMVIVATNSPDTLFPGVAPKVQG
                     LLKAENAGAFDVQSGCTSGVYSIVLAVNGIASGLWKNVLVIGAEVLSPLIDWTDRNTC
                     VLFGDGAGAMVLAPSNGNGRFISAQLKSDGTKHDQITFLGGLIENPASHETIDQKKHY
                     VAMKGNDVFKFVNRVIPPFLKTLCEDAGMLPEGIDWWFFHQANQRIIERTAERLGINP
                     ERVFINLDEYGNTSAASVFIALSEFMKQGKLLRGQKLLFTAFGAGMTYGGVIYES"
     gene            complement(6090..>6468)
                     /locus_tag="PK442_01220"
     CDS             complement(6090..>6468)
                     /locus_tag="PK442_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOO86296.1"
                     /translation="NFTGYVEGDDIPYSRADVVVCDGFSGNIVLKFAEGLMTAVASML
                     KEEIGHRILPKVGMVFMLPMMKDLIARFHYEKHGGTPLLGVNGAVIKAHGRSKAPAIV
                     SALRVARNFVKQNGVELIREELG"
BASE COUNT         1517 a         1777 c         1550 g         1624 t
ORIGIN      
        1 agaaagaaaa ggaaaagaat tcgtttcgtt ttcatggcgt ggctgtctcc ttcgtgaaga
       61 aagtataccc ttctcttgcc gcattgaatt ctgccgcttt tttcgatgtt cctcttcctt
      121 ctccagccgg ggaacctcca aggagcactc gcacatgaaa tagaggttga tgagaaggac
      181 cggttacgga aagaatttcg taggaaggaa tgccgatgcc tctctcttga gcgagaatct
      241 gaagctcggt tttcgggtcg gcaggttccg gagaagaaag aaacattaga aaatcaagat
      301 atcttagtat aacagcatca gcggctttat atcctccatc gagaaaaact gctccgaaaa
      361 gggcttcagc ggcatcttcg tagattgaac tgcggacagt gccgccagac attcctttcc
      421 ctgtcctcag aagcgttgga atatcaataa attccgccca tcggcgaaga gagtttccgc
      481 aaacaagtgc ggctctcctc cgtgaaagag ctccttcatc aagctgtttt tccgagataa
      541 aaagcaaatg ggaagcgctg agctgaagca ccgagtcgcc gaggaattcc aggcgttcat
      601 tgtgatcgtt cccgccgtgt tcgtgcgtaa acgaagaatg cagcaatgcc attcgcaaaa
      661 gagtgggatc gtcgaaaaag taaccgatac tctcttgcag cttttcaagc tttccgttgt
      721 accagtcttc gaaattcatt cttcctaaaa aagaaaaata cagccccgaa aaaacggggc
      781 tatactttct ttttactcct caaacctctg aagcgcaata acaccgttgt ggccgccgaa
      841 gccaaaactg ttcaccagaa gcctggaaac atgagctttc acgggggaaa cccccacaac
      901 gttgatagcg cactcaggat cgagatgctc caagttgatt gtcggatgaa taataccctc
      961 ttcgatagtc tgaagagcgg ctacggtctc caaggcgccg gctgctccaa ggcaatggcc
     1021 tatcatggat tttgttgagt ttacataagc cttgtctgta tgatcaccga gaacactgcg
     1081 aatagctatg gcttccattt tgtcattaag aggcgtggat gttccgtgcg cattgatgta
     1141 ctcgacgtct tcaggaagcc acttcgcatg cgtcatagcc ttgcgcatgg cccgggcagc
     1201 gccgtcgccc tcgggatctg gagctgtaat atggcttgcg tcgcaagtat tgccgtaacc
     1261 gacgagctcc gcatatatgt gagcccttcg tcgttttgca tgttccagtt cttcaagaac
     1321 cagaacgccg gcgccttcac ccatgacgaa gccatctctg tcgacatcga acggacggga
     1381 agctctctcc ggttcgtcca tccttgtgga aagggctttc atcgccgcga atcctgcaat
     1441 actgatggtc cgaagcgctg cttcagttcc ccccgcaagg atgacgtcgg cgtcgtcgcg
     1501 aagaattgtg tgatatgctt cacctaaact atgaacagag gtcgcgcatg cagtgacaac
     1561 acacatgttg ggccccttcg cattgtgttt gatggcgacg tatgccgtcg acatgttgct
     1621 tatcatcatc ggaatgaaga aggggctcac cctcttgggg cccttctcca tcatcgtctt
     1681 gaaattgttg aatgtggtct ctattccgcc ctgaccactt ccaatatata ctccgaattt
     1741 atatggatcc aggcttccag tatcgagctt tgcatccgcc accgcaagac cggccgcggc
     1801 gacagcaaat tgaatgactc tatcggaacg tttggcttct ttattgtcca tccattggga
     1861 ggggtcaaaa tcctttatat ccgcgccaaa tgggacggga ctatcgcttg catcaaacgt
     1921 ggtcagagga ccaataccgt ttttgccgtc tttcaaagct tgccagtagt cttcttttcc
     1981 tgtggcgata gggctgacaa ctccgagccc tgtaattaca acccttctca aaatgcttca
     2041 ctcctccacc gcaagggtcg atacagacga tgtttcgcgg aaaaactccg caataggggg
     2101 gatgcgcgga agcgctcccc ccttcgcttc tcggcgacct actcgtccac accgagcttg
     2161 ctcaatgtgt aattcatcgc ctcaccgact gtggtgagtt tctcggcgtc ttcatcagga
     2221 atctctatgt cgaattcctc ttcgattccc ataatgagtt cgacaatatc aagggagtcc
     2281 gccccaaggt cttcgacaaa ggaagcttca gcaactatct ggtcctcttc cacgtcaagc
     2341 ctgtcgataa taatctcctt caaccgagcg agcatttctt cttttttcat tctcgttcac
     2401 cccccttcac tctcttttta gcacatggtc atgccgccgt cgacggcaat aacctgtccc
     2461 gttatatacg acgcttcatc ggaagcaagg aaacacgcga tatttgcaac gtcttcgggt
     2521 gttcccggcc ttccggcggg cacttgtttc aaaagagttt ccctcgcctc ctgcggcaac
     2581 gcatcagtca tatccgtgcc aatgaaaccg ggtgcgagag cgtttacggt gatccctttt
     2641 gcggcatact ctctggcggc cgattttgta aaacctatga ctccagcttt tgaggcgcaa
     2701 tagttcgcct gcccggggtt gcctatcagg ccgacaaccg aggcgatatt tatgacacgt
     2761 ccccacttgg cccgtaccat tctcctgaga gcttctctgg tgcaataata caccgaggag
     2821 agattcgctg tcagcaccga attccaatct tcgtctttca ttctgatgag cagggcgtcc
     2881 tttgtaattc cggcattgtt cacaagtacg cttacgttcc cggctttttc attgacaaaa
     2941 gagaataccc gttttacctc ttcgggatcg gacacatctc ctttgacggg gaaggcgctt
     3001 cctcctccgg cggcaatgat atcacaaact tccaaggctg ccttctcgct gctgcgaaaa
     3061 ttcacggcga caaaaaaacc gttcttcgcc aggcggattg ccacagcccg cccgatccct
     3121 ttgcctgcgc ctgttacaag agcgatgcgc tgctccatat cacatcatct cctttaaaag
     3181 agccgctgtt ttttcaaggg tagcaagagt tccgcacgga tatgccgtga gacccttgcg
     3241 gcacttcttc accaatgctc ccaaaactgt accggggcct atttcaaaaa aagtgtctac
     3301 cccggaatct gccatccagt gaacagattt ctcccagagg accggcatat acgtttgatg
     3361 gaagagggcg tcctgaatgt cttgtatgtt ggctacaggc agcgcgcgca cgttggagac
     3421 aataggtgcg gagggattcc cccacgcgca ctgcttaaaa gctgtaagaa gctgatctgc
     3481 cacagggcgc atcaaacggc tgtggaaggg agcgctgaca ttcaagagca ctgttttttt
     3541 ggcgcctttt tcttttgcca aaagcatcgc tttctcaaca aattctgttt ctccggagat
     3601 gacaatctga ccgggagaat taaagtttgc gggctgacac tcgtgcaaag gagcgacctc
     3661 ctggcaaatg tcggagacag cctgcgcatc aagtccaagt attgccgcca tcgcgccctt
     3721 gccctcagga accgcttcct gcatgagcct cccacgaaga tgaaccaggc ggacgccgtc
     3781 ttcaagagag agcgttcttg cagctaccag agccgtatac tccccgagac tgtgtcctgc
     3841 gagagaagct ggttgaagat gtttccccac aagttcctct aaaacggaga ttgcggctat
     3901 gcttgcgacg agaatggcag gttgcgtata tgccgtcctc cgcagttcct cttcagggcc
     3961 atcaaaaatt atctttgaaa gagaaaagga gagtgcatca tcggcacgct gaaagacttc
     4021 cgcagcactt ttaaatgcat cacgaatgtc tctgcccatc cctacttctt gagatccctg
     4081 accggggaag atcagcgaat atgacatctt ttcacctcat tccaaagaaa gagctgcaga
     4141 aagcccctga ctgattattt ctgcatcacg aaaaagattc cggataatct ctgcggcagg
     4201 aaggatatcg ttgaccaggg cggcgctctg tccagccatg agagaccccc attcgacgtc
     4261 gccttcgaca acggcggcgc gaagacgtcc tgctcccagc ttttcatact cctcaaccgg
     4321 cgcgttctct ttttcaagct cttcaaaacg gctggtaagc ttgtttttta gacagcgcac
     4381 agggtgccca gtaatacggc cggtaatgac gttgcttctg tcccgagctg cgagaatagc
     4441 ctctttgtag gacgggtgga cagttgactc tgtgcaacag acaaaccgtg ttccaacctg
     4501 tacgccttcg gcgccgagcg caaatgcagc cgcgacggct cttccgtcgg cgataccacc
     4561 tgctgcaacg acaggaatct ctacagaatc aactatttga ggtacgagaa ccatcgtcgt
     4621 tagttcgccg atgtgtcctc ccgcttccat gccttctgcg actaccgcgt ccgccccctg
     4681 cttctccact cgtttagcct gtgcaacgga agcaataacg ggaacgacga tagttccgga
     4741 cggtttcaag cgttcaatga ccttgccggg acttcctgct cctgtggtga caatcggaac
     4801 gctgtatttt gcagctaact cgattacgtc ctcaacagtc ggtgacatga gcatgatgtt
     4861 cagcccaaaa ggtctgtcgg taagcgtacg cactttaaca agctgttgtt cgaggagttc
     4921 cgggggcata tttgcggcgg caatgactcc tagccctccc ccattgctta ccgctgctgc
     4981 gagctcagca tccgcaaccc aggccatacc tccttgaata agaggatagg agcatccgag
     5041 gagtcggact atgcggtttc tacggtgcga cgatgttgta ctatgattca tagatgacac
     5101 ctccgtatgt catacctgcc ccgaaagcgg tgaagagcag tttctgtccc cgaaggagtt
     5161 tcccttgttt cataaactcg gacagagcga taaagacgga tgccgcggat gtgttgccgt
     5221 attcgtcaag attgatgaat actctctcgg gattgatgcc gagacgctct gctgtgcgtt
     5281 caatgattcg ctggtttgcc tgatgaaaaa accaccaatc aattccctca ggcaacatgc
     5341 ctgcgtcttc gcacagcgtt ttcaaaaacg ggggaataac tcgattgaca aatttaaaga
     5401 cgtcattccc tttcattgcg acatagtgtt ttttctgatc gatggtttcg tgcgaggcag
     5461 ggttttcgat aagccctccg aggaacgtta tctggtcgtg tttcgtgccg tccgacttca
     5521 gttgagccga gataaagcgt ccgttgccgt tggaaggagc gagaaccatc gcgcccgcgc
     5581 cgtcgccgaa aagaacgcac gtattccggt cagtccagtc aataagcggc gaaagcacct
     5641 cggcgccaat gacgagaaca tttttccaaa gacctgaagc tataccgtta acggcgagga
     5701 ctatagagta cactccgctg gtgcatcccg actgaacgtc gaacgcccca gcattctctg
     5761 ctttaagcaa cccctggact ttcggggcta ctcccgggaa tagagtatcc gggctgttcg
     5821 tggcaacaat gaccatatcg agttcagaag gcaagatacc agcattttca agtgctgcac
     5881 gccctgcctt ttcggcgatt gtgctcgtga gctctcccgg ttcagcaatg tgtcttctct
     5941 gaatgccagt cctttcacga atccattcat cgcttgtatc gactatttgt gataagtcgt
     6001 tattcgtcac tacttttctc ggggcataca taccgctacc gcgaatggcg acgggcactc
     6061 cttgaaaaaa agacatgaga agacctcctt taccctaact cttcccgtat caactcaacg
     6121 ccgttctgct taacgaagtt tctggctact ctcagggcac ttacgatagc gggtgctttc
     6181 gaccgtccat gggcttttat tacagccccg ttgacgccca gaagcggcgt accgccgtgc
     6241 ttctcatagt gaaagcgtgc aatgagatct ttcatcatag ggagcataaa gaccattccc
     6301 acctttggaa ggatgcgatg ccctatctct tctttaagca tagaagcgac cgcagtcatg
     6361 aggccttctg caaatttgag aacgatgttt cccgagaagc cgtcgcagac aacgacgtcc
     6421 gctctgctat acgggatgtc gtctccttcc acgtatcctg taaaattg
//
LOCUS       DAOSWW010000030         6437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_7885_length_6437_cov_3.836258, whole genome shotgun sequence.
ACCESSION   DAOSWW010000030 DAOSWW010000000
VERSION     DAOSWW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7885_length_6437_cov_3.836258"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..436
                     /gene="yidD"
                     /locus_tag="PK442_01225"
     CDS             164..436
                     /gene="yidD"
                     /locus_tag="PK442_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOO86297.1"
                     /translation="MTPAAWVGITLVRGYQLFVSPLLGHNCRFYPTCSQYAIDAISVH
                     GFLKGSFLAVKRLCKCAPWHPGGYDPVPPAARREEQGAYSYKDGDL"
     gene            436..1224
                     /locus_tag="PK442_01230"
     CDS             436..1224
                     /locus_tag="PK442_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOO86298.1"
                     /translation="MGSIWKMGGDLMMRLMELVHGFTGSWGLAIIVLTLIVRLLLHPL
                     TKKQMTSMQKMQKLQPRIKMLQEKFKDDKESLNREMMALYKENKVNPAAGCLPLLVQL
                     PVFILLYQVLTNYDFSGVSFLWIRLDGSVLTTLGEALKLTVEKDQLGIMTVLYGLMAN
                     PYALLNVSVYLPNLLLLLGIGFLTWYQQQLTASGNPQMAMMNWFMPLFLTFICLSLPG
                     GVLLYWGVSSLLGVVQQLSMSRKTKAEMQQKPVLFKDKPTRSGD"
     gene            1391..2041
                     /gene="jag"
                     /locus_tag="PK442_01235"
     CDS             1391..2041
                     /gene="jag"
                     /locus_tag="PK442_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOO86299.1"
                     /translation="MNDDILVLDVSSVEEARKIASAEWDLDSADLTIKVIEEEKSFFG
                     LLGRKLRVEVRPVAPLHVLRGRNTIDSLLKMMELHITSEIADENRINLSGPDAGIIIG
                     KYGETLKSIEYLLNLVSRGADEGRRIVLDSDGYRERRELSLQRLALAAARKCPRKGRP
                     AHLEPMTSWERRIVHLTLKDSPDVETRSVGESPSRKVVIYPLRRSSYSGRPPRKDS"
     gene            2041..2820
                     /locus_tag="PK442_01240"
     CDS             2041..2820
                     /locus_tag="PK442_01240"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOO86300.1"
                     /translation="MYPVLFHIGDNPVHSYYLLWTLALTLAVVFSRRRMTFFYGIDDD
                     DARSVIIWAFIGMLLGARAGSVYDSWAIYSADPLKILRIWEGGLSAVPAFLGAGAVSF
                     VYSKRRKIPYWMIVDAGALPAALTVAIGRWGCFLNGCCTGIETTVSWGVRFPSDALGL
                     LRHPTQLYYSFGALFIAALLQWTESSWLGYHNDRRIRGAVLCPLFAILYSLLRLAADP
                     FRSDFTRIGMQTNRSVLFVVLGISMLWLGYSVFYEKKCIRS"
     gene            2915..4333
                     /locus_tag="PK442_01245"
     CDS             2915..4333
                     /locus_tag="PK442_01245"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOO86301.1"
                     /translation="MKGTKITSAFFGSVKVLFVLLLLVVPFLLTADRAFAQDPFTGNP
                     VAKIARELSPAVVNIDVETMVTRSVHPFADDPFFRQFFGEEQRRFSRTVPMKGRGSGF
                     IVSKDGHIITNNHVIDGADKITVTFSDGRTFEAKVLGKDPTFDLAVIKIDGADLPFLE
                     LGDSDATEVGEWVVAIGNPFGLEHTVTVGVVSAKNRSIHAGNVNFDGFLQTDAAINPG
                     NSGGPLLNLEGKVVGINTAIVPFAQGIGFAVPVNMAKQVMDDLIEFGRVKRAWLGVYI
                     QPLTKEFAEAYGVKEENGAVVSDVLRDSPAEKAGLQRGDVILEVDGSGVKNHQEFVMK
                     IRQKLAGDKVTLTLIRQKKNIKVDIPLGEMPGNEKQAASRTGGSKMLTSIGLEAGEMT
                     DDIRKQNNLRKDEGVLITAVEDGSMAQRAGIRENDVLLEVNGVRLSGLSDLDKADVKG
                     NSVVLLIMRDSRSFFVSIRTGQ"
     gene            complement(4409..5086)
                     /locus_tag="PK442_01250"
     CDS             complement(4409..5086)
                     /locus_tag="PK442_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO86302.1"
                     /translation="MSVLIIEDNRDLVQVLAEVLNENGFSVESAHTGEEGLEKALLSR
                     FDCIILDIVLPGINGFQVVEEYRKEDGSSPVLILSARDSVEDKVEGFNRGADDYMVKP
                     FDSRELIVRVRSLIRRAADKQSPLLACGRLTLDPVTRECRVDGTFVNLRRREFDILET
                     MLRHANQVFTRERLVASVWKKEYDGRSNVVDVHVKYLRDKLRAHGFDTAIETIRGVGY
                     KIRCSEE"
     gene            5346..5612
                     /gene="rpsO"
                     /locus_tag="PK442_01255"
     CDS             5346..5612
                     /gene="rpsO"
                     /locus_tag="PK442_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOO86303.1"
                     /translation="MLQGEKKQGIITEFQVHTTDTGSPEVQVAMLTQRIRELTEHLKI
                     HTKDFHSRRGLLKMVGKRRRLLRYLKDKDFNRYRTLIERLGLRH"
     gene            5727..>6437
                     /locus_tag="PK442_01260"
     CDS             5727..>6437
                     /locus_tag="PK442_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOO86304.1"
                     /translation="MEMTFSVEIGGRPLEFSTGKVAKQAGGAVVAAHGETTVLTTACI
                     TDKPRTGIDFFPLLVDFEERFYSAGKIPGGFIKREGRPSETAILSCRMVDRSIRSLFD
                     ETMRHDVHVVSTVLSVDQVNPPNVLAINAASAALTISDIPWGGPVGAVRIGRLGDEFV
                     VNPTEEQMLESTLDLLVAGHLDGVTMVECGSKEIAEDILVDALELAQNEIKKIVGLFN
                     EMRAAVGREKLVLPSAPSF"
BASE COUNT         1388 a         1719 c         1846 g         1484 t
ORIGIN      
        1 ggccgggagt atggatagtt gcgggactca gcagcgcagg aatgggggcg cgctcgacgg
       61 atgtttacga agagctggcc cgacttctcg aagggggagg gtttctggag ccggaatggc
      121 cgggagcgaa ctgggaaaat ccgtcggagg gtgcaaaaga aaaatgacgc ccgctgcatg
      181 ggtcggaatt acactcgttc gggggtacca gctttttgtg tccccgctcc tcgggcataa
      241 ctgcagattc tatccgacgt gctcgcagta tgcgattgac gcgatttccg ttcatgggtt
      301 cctgaaaggt agctttttag cggtgaaacg gctttgcaag tgcgccccgt ggcaccccgg
      361 agggtatgat cccgtaccgc cggcggctcg aagagaggag cagggggctt attcgtacaa
      421 ggatggtgat ctatagtggg ttctatttgg aagatgggcg gggaccttat gatgaggctg
      481 atggagcttg ttcacggctt cacagggtcc tgggggttgg caatcatcgt actcacgctg
      541 attgtccgtt tgcttctgca cccccttaca aagaagcaga tgacgagcat gcagaagatg
      601 cagaaactcc agccccgtat caagatgctg caggagaagt tcaaggacga caaggagtcg
      661 cttaaccgcg agatgatggc gctctacaag gagaacaagg taaacccggc cgccggatgc
      721 cttcctcttc ttgtccagct tcccgtcttc attctgctct accaggtgct gaccaactac
      781 gacttttcag gcgtctcttt cctctggata cggcttgacg gttccgtgct cactacgctc
      841 ggcgaggcgc tcaagctgac tgtcgagaag gatcagctcg gcatcatgac cgtgctctac
      901 ggccttatgg cgaacccgta tgcgttactg aacgtttccg tctatcttcc caacctgctg
      961 ctgcttctgg gaatcggatt ccttacgtgg taccagcagc agctaacagc gagcgggaat
     1021 ccgcagatgg cgatgatgaa ctggttcatg ccgctcttcc tcaccttcat ctgtctcagc
     1081 cttcccggag gagtcctttt gtactggggc gtctcctctc ttctcggagt ggtgcagcag
     1141 ctgagcatgt cccggaagac caaagcggaa atgcagcaga aaccggtact tttcaaggat
     1201 aaaccgacca ggagcggtga ctagctagag acgtgccggc tccgccttcg cggagcctcc
     1261 agccggcgag cggacgcccc tgattcgggg tgtccgcgta cgtcgtgcag catacagcca
     1321 cttcttctcc ttttcagggg aacggagagg ctttctccca gggagttctc tgaacggaag
     1381 gaggattgtt gtgaacgacg acatacttgt tctggacgtt tcttcggtgg aagaggcccg
     1441 gaaaatagct tcagccgagt gggatcttga ctctgctgat ctgacaatca aggtaatcga
     1501 ggaggagaag agttttttcg gtctcctcgg cagaaaattg cgcgtggagg tgcgtccggt
     1561 cgcccctctt cacgttctcc ggggcagaaa taccatcgac agcctgctca agatgatgga
     1621 actgcatatt acctcggaaa tagccgatga aaacaggatc aatctctccg gaccggatgc
     1681 gggcatcatc atcgggaagt acggagagac gctcaagtcg attgagtacc tgcttaatct
     1741 tgtctcaaga ggagcggacg aggggcgtcg cattgttctg gacagcgacg ggtacaggga
     1801 gcggcgagag ctgagccttc agcgcctcgc gctggccgcc gcgcgcaagt gcccccggaa
     1861 ggggagaccc gcgcaccttg aaccgatgac aagctgggaa cggcggatcg tccatcttac
     1921 gctgaaagac agcccggatg tggaaacgag gtccgtaggc gaatcgccct cgcgaaaggt
     1981 tgtcatttat ccgcttcgga ggagttcgta cagcggaaga cccccccgaa aggattcctg
     2041 atgtacccgg ttctctttca tatcggcgac aacccggtcc acagctacta cctgctgtgg
     2101 accttggctt tgactctggc cgtcgttttt tcgcgccgga gaatgacgtt tttctacgga
     2161 atagatgacg acgatgcgcg ctcggtgatt atctgggcct tcattggaat gcttctgggc
     2221 gcgcgcgcgg gaagcgtcta cgactcgtgg gcgatctata gcgcggatcc cctgaagatt
     2281 ctcagaatat gggagggagg gctttccgcc gtccccgcct ttctgggcgc cggggccgtt
     2341 tccttcgtct attcaaagcg ccggaaaatc ccgtactgga tgatcgtgga cgccggagcg
     2401 cttccggcag ccctcaccgt cgcaataggc cgctggggct gttttttgaa cgggtgctgc
     2461 acagggatcg agacgacggt ttcatgggga gtccgtttcc cctcggatgc tttggggctt
     2521 ctccgccatc caacgcagct ctactactct ttcggagcgc ttttcatcgc cgctcttctc
     2581 cagtggacgg aatcctcgtg gcttggctac cataatgacc gcagaatccg gggagcggtt
     2641 ctatgtcctc tcttcgcgat actttattct ctgctgcggc tggctgccga tcccttccgg
     2701 agcgacttta cccgcatcgg gatgcagacg aaccgctctg ttctcttcgt tgtgctcgga
     2761 ataagcatgc tctggctcgg gtattccgta ttctacgaga aaaaatgtat acgaagttga
     2821 tatgaaatgc gtattcatgg ataggtaaaa aaaacgatgt atccagccgc cgggcgcata
     2881 caataaaagc atcttgattc ggaggtgaca caccatgaaa ggaacaaaaa ttacgagtgc
     2941 gttcttcggt tccgtgaaag ttttgtttgt gcttcttctg cttgtcgtgc cctttttgct
     3001 gacggcggat cgggcattcg cgcaagatcc gtttaccggg aaccctgtcg ccaaaatagc
     3061 aagggagctt tctcccgcgg tggtcaatat cgatgtggag acgatggtca cccgttccgt
     3121 ccatccgttc gcggacgacc cgtttttccg ccagttcttc ggtgaggagc agcgccgttt
     3181 ctcccgtacc gttcccatga aagggagagg ctccgggttc atcgtgtcga aggacggaca
     3241 tatcatcacg aataaccacg tcatcgacgg cgccgacaag ataacggtga ccttctcgga
     3301 cggccgtacg ttcgaggcga aggtattggg caaggatcct acattcgatc ttgccgtcat
     3361 caagatcgac ggtgcagacc tcccgtttct tgaactcggc gattcggacg cgaccgaggt
     3421 cggcgagtgg gtagtcgcga taggaaatcc gttcggactt gaacatacgg ttaccgtcgg
     3481 tgtcgtctcg gcgaagaacc ggagcattca tgccggaaac gtgaacttcg acgggttcct
     3541 tcagacggat gccgcgatca accccggcaa tagcggcggt cctcttctga acctggaagg
     3601 gaaggtcgtc ggaatcaaca ccgccatcgt tccgttcgcc cagggtatcg gcttcgctgt
     3661 gccggtgaat atggcgaagc aggtcatgga cgatcttatt gagtttggcc gggtgaagag
     3721 agcatggctc ggcgtgtaca ttcaaccgct gaccaaggag tttgccgagg catacggtgt
     3781 gaaggaagag aacggggccg ttgtcagcga cgtgcttcgc gactcacccg ccgaaaaagc
     3841 cgggctccag cggggcgatg tcatccttga ggttgacggt tccggcgtta agaatcacca
     3901 ggagttcgtg atgaagattc ggcagaaact cgccggagac aaagttaccc tgacgcttat
     3961 ccgccagaag aagaatatca aggtggatat ccccctcggc gagatgcccg gcaacgaaaa
     4021 acaggccgct tcccgcaccg gcggatcgaa gatgctcacc tctatcggat tggaagccgg
     4081 cgagatgacg gatgatatcc ggaagcagaa taaccttcgc aaggatgagg gtgtgctgat
     4141 caccgccgtc gaggacggct ccatggcgca gagagcgggt atccgcgaga acgacgtgct
     4201 tctggaagtg aacggcgtcc gtctttccgg cctctcggat ctcgacaagg cggatgtcaa
     4261 gggcaattcc gttgtgctgc tgattatgag ggattcgaga tccttcttcg tctccatacg
     4321 gacagggcaa taaagaagag tcagtaaaag aagaagagcg aagcgcccct gcacgggcgc
     4381 ttcgctcttc ttctggagta ggtttctcct actcttcgga acaacgaatc ttgtatccca
     4441 cacctcgtat cgtttcgatg gcagtatcaa atccgtgagc cctcagcttg tccctgagat
     4501 acttgacatg gacgtcgaca acgttgcttc tgccgtcgta ttcttttttc cacacggatg
     4561 cgacaagacg ttcccttgtg aacacctggt tggcgtgccg gagcattgtt tcaagaatat
     4621 cgaactccct gcgtctgagg ttgacgaaag ttccgtccac cctgcactcc cgcgtcaccg
     4681 gatcgagcgt gagccgaccg caggcaagca gcggcgactg tttgtcggcg gctctccgga
     4741 tgagcgatcg tacccgcacg atgagttctc ttgagtcgaa cggtttcacc atgtagtcgt
     4801 cggctccgcg gttgaacccc tccaccttgt cctccaccga gtcgcgggcg gacaaaatga
     4861 gtaccggcga acttccgtct tcctttctgt attcctccac gacctgaaac ccgttgatcc
     4921 ccgggaggac gatatccaaa atgatacagt cgaatctcga gagcagcgct ttttcaagcc
     4981 cttcttcccc tgtgtgcgcg ctctcaacgg aaaaaccgtt ttcgttgagc acttcggcga
     5041 gcacttgcac tagatctctg ttgtcctcga tgatgagaac ggacacgttg cttcccccct
     5101 aatgaaaaaa ctctggtaaa ataatcaaaa agattaaagc acatccagaa ttaaaagtcg
     5161 agtgcaagat gagatcactt tttctttttc tgaaatccag ctgaaatttg ctctcttccc
     5221 gctgggcgtg cgtgatgatt tgctttattt tctgcaagat gatagtatta ctggcgaccc
     5281 ttacccggat ttgcgcatag agcgctcttc tgagttacgg gattctgtct acgaggtgaa
     5341 gaaacatgtt gcagggagag aagaaacagg gaattattac ggagtttcag gtgcacacga
     5401 ccgacaccgg gtcgcccgag gttcaggtgg cgatgctcac gcagcgcatt cgcgagctta
     5461 ccgaacatct caagattcac acgaaggact tccattctcg ccgtggtctt ctgaagatgg
     5521 tcggcaaacg ccgcagactt ctccggtatc tgaaggacaa ggatttcaac cggtacagaa
     5581 cgcttatcga gagactcggt ctgcgtcact aaaaaaggtc gatttccggt gcgagggcga
     5641 aaagtcctcg cacttttttt gttcgattgt ggtattctta ctccgcatac aataaaaaga
     5701 gctgaatttt gaggaggaag aagtgtatgg aaatgacgtt ttcagttgaa attggaggcc
     5761 gtcctctgga gttttccacc ggaaaagtgg caaagcaggc aggcggagcc gttgtggcgg
     5821 cacatggcga aacgacggtg cttaccacag cctgcatcac cgacaaaccg cgcaccggga
     5881 tagatttttt cccgctgctt gttgattttg aagagagatt ttactccgcc gggaagattc
     5941 ccggagggtt tattaagcgc gaaggtcgcc cctccgaaac ggccattttg agctgccgga
     6001 tggtcgaccg ttccatccgc tcgctttttg acgagacgat gcgccacgac gtccatgtgg
     6061 tttcgacggt gctctccgtc gaccaggtga acccgcccaa cgtgctcgca atcaacgccg
     6121 catccgccgc gctcaccatc tcggacatcc cgtggggagg cccggttgga gccgtaagga
     6181 tcggccgtct cggcgacgaa ttcgtggtga accccaccga ggagcagatg cttgaatcca
     6241 ctctcgacct tctggttgca ggtcatcttg acggtgtgac gatggtcgag tgcggctcga
     6301 aagagatcgc ggaagacatt ctggttgacg cccttgaact ggcgcagaat gagatcaaga
     6361 agatcgtcgg tcttttcaac gagatgcgcg cagctgtcgg acgcgagaag cttgtgcttc
     6421 cgtcagcccc ctccttc
//
LOCUS       DAOSWW010000031         6267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8191_length_6267_cov_4.541050, whole genome shotgun sequence.
ACCESSION   DAOSWW010000031 DAOSWW010000000
VERSION     DAOSWW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8191_length_6267_cov_4.541050"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="PK442_01265"
     CDS             <1..138
                     /locus_tag="PK442_01265"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOO86305.1"
                     /translation="ELNCGQMVLEVERAVHGKAKVVAQNLVNGELFKPDDILSVIEEV
                     A"
     gene            140..970
                     /locus_tag="PK442_01270"
     CDS             140..970
                     /locus_tag="PK442_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOO86306.1"
                     /translation="MPSQEVFNWLRTRFFPHIWCPGCGHGIIMHSLLRALVDLKKKKT
                     ETVIASGIGCSSRMVGYIDACTVHTTHGRSLAFATGIKLANPELTVVDVMGDGDCTAI
                     GGNHFIHACRRNIDITAIVMNNNIYGMTGGQVSPTTPVGSLATTSPYGAIDPSFDICK
                     LAEGAGASYVARTTIANPKQAEQFIKAGINNKGFSVVEILTHCHTQFGRRNKRRTPID
                     NVNFFKANSVPLNKAQEMSPEELAEKIVVGEFVKREIPEYTAQYLSIIERVGGLKNGK
                     "
     gene            960..1523
                     /locus_tag="PK442_01275"
     CDS             960..1523
                     /locus_tag="PK442_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO86307.1"
                     /translation="MASRYEIRFAGSGGQGVILAAVVAGEAAALFEEGLSVVQTQSYG
                     PEARGGKSKAEVVISTEPIDYPKTMKPNLQVILTQQAAEEYAAETLPGGRIIYDDFFV
                     TSFPQVDANIYYLPIVRTAREKIGKEIVTNMVALGAVARVLELEKIVRPESLKKAILA
                     KVPEGTKQLNAQAFDEGYLLFKNSVHL"
     gene            1733..3109
                     /gene="rlmD"
                     /locus_tag="PK442_01280"
     CDS             1733..3109
                     /gene="rlmD"
                     /locus_tag="PK442_01280"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOO86308.1"
                     /translation="MIRVYEKGASVLLRTEKISSDGSGIARTPEGFVIFIPGALPGET
                     LKATVMLRKKEYAIAFPDELLEPHPLRRQPFCPVFNSCGGCQLQHASYALQLELKRAI
                     VQDAFERIYKRPFPPVTSCNPSPEQIRYRNKTSLPVRNREGKTVMGYFARRSHDVVPI
                     KSCPVAAQHIDRAFSAVAESLPVLGLVPYNEKNGKGMLRHAIFRQSIENNDTLVSFVL
                     ADALSSRKKAILEERLLPALQEALPNLRSLTLNINSARNNVIVGPKTEVLFGDGLIEE
                     TLASFRFEYDTTAFFQVNSRQACILYEFVVDLAGLTGTERVLELFSGIGTLTSFLAAR
                     SLHVTAVEEWDSSVRMMRSNLSRNGVGEKTAIIAGAVEKVAPSLEGTFDVIVLDPPRT
                     GCDASVLHKVLSLNPRRIIYVSCNPATLARDAGILTDQGFVLDTLACFDLFPQTVHVE
                     TVARFFRI"
     gene            3298..3373
                     /locus_tag="PK442_01285"
     tRNA            3298..3373
                     /locus_tag="PK442_01285"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3331..3333,aa:Gly,seq:ccc)
     gene            3586..3918
                     /locus_tag="PK442_01290"
     CDS             3586..3918
                     /locus_tag="PK442_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HOO86309.1"
                     /translation="MAANLAEEIKEVEARSKDIVKDAKNGAAQMINEAQNEIERRTKE
                     AKQKAFRLFKENTASVEREAEEKAKQSVEAGQKKSEQLAKQYGQKVNSAAQWISEEVI
                     SRYGSSEN"
     gene            3899..5911
                     /locus_tag="PK442_01295"
     CDS             3899..5911
                     /locus_tag="PK442_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOO86310.1"
                     /translation="MAVARIKKAELYVHKADLDNVLHALQKTGSFEVVPFGSESVSES
                     TAPVHTDLSRVEGLLSEARFLLRFLEPYFKDNVSSLARTMGERETISLHDGKTLEDRT
                     DLMSLSASVRSLERRLMEIRSELTQIASTETLLNALSDFPYPFELLTSGTERVRGILG
                     TMPVSKIPAWREETEALLLSMGEVFTSTQGEKDTEGWIAVFFDEQKTAQITDLLARNS
                     FSRVDVPLSLKKTPEEERLALSDRKVALASEEERVLAEIAGAAEQWVPSVRILSDYWT
                     VLRDRYQAISSGSSTEQVIMLRGWVPASAVQRLTKAITPFGLSVELFLFDPKPEDSPP
                     SHIVNSAWCFPFENLTRLYGAPKYGEIDPTPLLAPFFFIFFGMCLGDAGYGAIMIGFF
                     LWFLRKYKKMPESFKGFFKLFVLSGVSTIVVGALTGSWFGDLVDAFSIFAFLRPLKNF
                     MLVLNPMENPMIFLAISLALGILQLFFGLFIAFYDALRKGNYMGAFADTGGWIVFLTG
                     LLLWGGVASGFISAQTSFLAKTLTFLGAAVLIATQGREKEGIIGKAVSGLLSLYSVTS
                     YLGDVLSYSRLLALGLATSAVGVIINMLAGLAGDVPYIGWIIALLLLIGGHIFSIAVN
                     VLGAFVHSLRLQYVEFFSKFYTGGGTVFSPLTYKTAFVAIKNEPEE"
     gene            5957..>6267
                     /locus_tag="PK442_01300"
     CDS             5957..>6267
                     /locus_tag="PK442_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOO86311.1"
                     /translation="MDLLGIMLAILGAAMAAGWAGAGSAIGVGIAGEAGAGVMTEDPG
                     KFGLVLLLQALPGTQGIYGLLIAFFAILKVGLLGGGAGVTVNVWQGLGIFFACLPIAV
                     GG"
BASE COUNT         1568 a         1544 c         1565 g         1590 t
ORIGIN      
        1 gagttgaatt gcggccagat ggtgctcgaa gtcgaacgcg ctgttcacgg taaagcgaaa
       61 gtcgtggcgc agaacctcgt taacggcgag ctgttcaagc ccgacgatat tctttccgtc
      121 atcgaggagg tggcctaata tgcctagtca ggaggtattc aactggctcc gcacgcgctt
      181 tttccctcat atttggtgcc cgggctgcgg acacggcatt attatgcact ctcttttaag
      241 ggcgctggtc gaccttaaga aaaagaaaac ggaaacggtc attgcgtccg gaatagggtg
      301 ttcgagcaga atggtcggct acattgacgc atgcaccgta cacaccacgc acggccgctc
      361 cctcgctttc gcaactggaa tcaaactcgc aaatccggag ctcacagtcg ttgatgttat
      421 gggagacggc gactgcaccg ctattggcgg taaccacttc atccatgcgt gcaggagaaa
      481 tatcgacata accgccatcg ttatgaacaa taacatctac ggtatgactg gcggccaggt
      541 ttcgcccacc acgcccgtcg gaagtttagc tacgacgagc ccctacggcg caattgatcc
      601 ttcctttgac atttgcaaac tcgccgaggg cgccggagca tcctacgttg caagaaccac
      661 cattgccaac ccgaaacaag ccgagcagtt tattaaggca ggcataaaca ataagggatt
      721 ttctgtcgtc gaaatcctga cgcactgcca tactcaattc ggcagaagaa acaagcggag
      781 aacacctatt gataacgtga atttcttcaa ggccaattct gtgcctttga ataaggcaca
      841 ggagatgtct cccgaggagc tcgccgaaaa aatcgtcgtc ggcgaattcg tcaaacgaga
      901 gattccggag tacacggcgc agtatctctc cataatcgaa agagtagggg ggctgaaaaa
      961 tggcaagtag gtacgaaatc cgtttcgccg gttccggcgg acagggcgtc attctcgcag
     1021 ccgtcgtcgc aggagaagcc gcagctcttt tcgaggaggg gttgagcgtt gtccagacgc
     1081 agtcttacgg tccggaggcc aggggaggca agtcgaaagc ggaagtagtc atctccacag
     1141 aaccgattga ctacccgaaa acaatgaagc ccaaccttca agttattctc acgcagcagg
     1201 cagcagagga gtacgcagcg gaaacactcc ccggcgggcg tattatttat gatgattttt
     1261 tcgtcaccag cttcccgcag gtggatgcga atatttacta tcttcccatt gtcaggactg
     1321 cgcgtgagaa gataggcaaa gaaatagtta cgaacatggt agctttggga gcagtggccc
     1381 gcgtccttga gctcgaaaaa atagtgcgac cggagtccct aaagaaagcg attcttgcga
     1441 aggttcccga aggaacaaaa caactcaacg cccaggcttt tgacgagggg tatctcctgt
     1501 tcaaaaacag cgtgcacctt taaaaaacac tttctccata atgaacgccc ttcttttcgt
     1561 aagaagggcg ttcaattttt gagcctttct ccacaccaaa tacaacgtaa tagcgaggaa
     1621 tacgcccaac gaaacttgga agcctttttc tgctctgaac ctttccctca ctcgaaagac
     1681 tttttccgct ctcttccgtg tataatgtgg tctgtgcgca aaaaaagagg agatgatccg
     1741 agtgtacgag aaaggcgctt ccgtccttct aaggactgaa aagataagca gcgacgggag
     1801 cggaattgcg cgcactccgg aaggttttgt tattttcatc ccgggggcgc tccctggaga
     1861 aacgctaaaa gctacagtca tgcttcgaaa aaaagagtat gcgattgcct tccctgacga
     1921 actgcttgaa ccgcaccctc ttcgtcggca gcccttctgc ccggttttca attcttgcgg
     1981 aggatgccag ttacagcatg cctcgtacgc tcttcagctg gaactgaaac gtgctattgt
     2041 ccaagatgct tttgaacgta tctataaaag accgtttccc cccgttacct catgtaatcc
     2101 gagtccagag cagataaggt acagaaacaa gacttccctt ccggtacgga accgagaagg
     2161 aaaaactgtt atggggtatt ttgcaagaag aagtcacgac gtagtaccga taaaaagttg
     2221 tcccgttgcc gcgcaacata ttgacagggc cttttcagcg gtggccgaaa gtctgccggt
     2281 tctcggtctc gttccatata acgagaaaaa cggaaaaggc atgcttcgcc atgcaatttt
     2341 ccgtcagagt atcgaaaaca acgacaccct tgtttccttc gtccttgccg atgcactctc
     2401 ttcccgcaag aaagcgattt tggaagaacg cctgctcccg gcgcttcagg aagcgcttcc
     2461 caacttacgc agcctgactc tgaacatcaa ctcggcacgg aacaatgtaa tcgtcggccc
     2521 gaaaacagag gtgctcttcg gagacggcct tatagaagaa accctcgcct cttttcgatt
     2581 tgagtacgat acaacggcat tcttccaagt caactcgcgt caggcatgca tcctgtatga
     2641 attcgttgtg gatctagccg gcctcaccgg cactgaacga gttctcgaac tctttagcgg
     2701 tataggtact ttaacttctt ttctcgccgc cagaagtctt catgtaacag cggtggagga
     2761 gtgggactct tcggtacgaa tgatgcggag caacctctcc cgcaatggag taggcgaaaa
     2821 aaccgcgatt attgcaggag cagtggagaa agtagcgcct tctctcgaag gtactttcga
     2881 tgtgattgtc cttgatcctc cgcgcacagg ctgtgacgct tcggtgctgc acaaggtcct
     2941 ttcgttgaat ccacgccgga ttatctatgt ttcgtgtaat ccggcaaccc tggcaaggga
     3001 tgccggtatt ctcactgatc aaggtttcgt ccttgatact ctggcatgtt tcgatttatt
     3061 tccccagacc gttcacgtcg aaaccgtggc gcgattcttt cgtatctaat caccacgtgt
     3121 ttttcattgg ccagagggaa aacgttaccc gggcaacgca gttatttcta agaacgcata
     3181 ctactgagaa tatcctctcc agtctgtgtt tcaacaaatg aaatcaggtg tgtcaggaaa
     3241 aagatgttgc caaaacagga tacgacggat ataataccat cgttcaagca aaagagggcg
     3301 gacatagctc agctggtaga gcattagctt cccaagctaa gggtcgcggg ttcgaatccc
     3361 gttgtccgct ccaattcttt tccccaatcc cctgatactg acttttctcc tgaaagaaaa
     3421 tattctttaa gactcttgcc ttgtttcgtt ttgttgatta tgatctggac atattgttac
     3481 gtttgacaca aattttgtct ggtaataaaa tagtcactgt ttttatgttg accgtgtgca
     3541 cagcgtgtgc actcgcgttc aatccaagga aggaggtggg tttatatggc agcaaatctg
     3601 gctgaagaaa tcaaggaagt cgaagctcgt tcaaaagata ttgtgaagga tgcaaagaac
     3661 ggggctgctc aaatgatcaa cgaagctcag aacgaaattg agcgccggac caaggaggcc
     3721 aaacagaaag cattcaggct tttcaaagag aatacagcct ctgtggaaag agaagcggaa
     3781 gaaaaagcaa aacagtctgt tgaggccggc caaaagaagt ccgagcaact tgccaagcag
     3841 tacggacaga aggtaaatag cgcggcacaa tggatttcgg aagaggtgat ttctcgatat
     3901 ggcagtagcg agaattaaaa aagccgagtt atacgttcac aaagccgatt tggacaatgt
     3961 cctgcacgct cttcagaaga caggatcctt tgaagttgtc cctttcgggt cggaatcagt
     4021 gtcagaaagt accgcgcctg ttcacactga cttatcacgg gtggagggac tactcagcga
     4081 agcgcgtttt ctccttcgat tccttgaacc ttatttcaag gacaatgttt cttccttggc
     4141 tagaaccatg ggagaacgag aaacaatctc gttgcacgat ggaaaaactc ttgaagacag
     4201 aacagatctc atgagcctat ctgcgagtgt gagatcgctt gaacgccgcc ttatggaaat
     4261 tcgctctgaa ctcacacaga tcgcatcgac ggagacgctt ctgaacgcgc tctccgactt
     4321 cccttatccg tttgaacttc tcacctccgg aaccgaacgc gtgcgcggca ttctgggaac
     4381 gatgcctgtt tcgaaaattc cggcttggag agaagaaact gaggcgcttc ttttatctat
     4441 gggtgaggtt tttacctcaa ctcagggaga aaaggatacg gaagggtgga ttgctgtctt
     4501 tttcgacgaa caaaagacgg cgcaaatcac agatcttctt gctcgcaact catttagcag
     4561 agtggacgta cctctttctc tcaaaaaaac accagaggag gaacgactcg ctctctcgga
     4621 cagaaaggtc gccttggcat ctgaagaaga acgcgttctt gcagagattg ccggcgcggc
     4681 ggaacagtgg gttccctcag tgcgtatttt gagtgattac tggactgtct tgcgtgacag
     4741 gtaccaagca atttcttccg gttcttccac agaacaggta atcatgcttc gcggctgggt
     4801 tcccgcatct gccgttcaga gactcacgaa ggcaattacc cccttcggtt tatctgtaga
     4861 actctttctg tttgatccga aacctgaaga ctcaccgccc tcacacatag tcaactcggc
     4921 atggtgtttc ccgtttgaaa acctgacgag actctacggc gcgcctaagt acggagaaat
     4981 tgaccccaca ccgctgctag caccgttctt tttcatcttc ttcggtatgt gtcttggcga
     5041 tgccggttac ggagctatca tgataggatt cttcttgtgg tttctcagaa agtataagaa
     5101 aatgcccgag agttttaagg ggttcttcaa actcttcgtt ctttccggtg tttccacaat
     5161 cgtggttggg gcgcttaccg gaagctggtt cggagatctt gtagacgctt tttccatttt
     5221 tgccttcctg cgtccgctaa agaactttat gctcgtcctt aaccccatgg agaatcccat
     5281 gattttcctt gcaatttcac tcgcacttgg cattctccag cttttcttcg gactctttat
     5341 tgcattctac gatgctctgc ggaaagggaa ctatatgggg gcctttgcgg acacgggcgg
     5401 ttggattgtc tttcttacgg gactgcttct ttggggagga gtagcaagcg gttttatctc
     5461 cgctcagaca tctttccttg cgaaaacgct tacgttcctt ggagctgcgg tgctcatcgc
     5521 aacacaaggg agagaaaaag aaggcattat cggaaaagcg gtttccggat tgctgagtct
     5581 gtacagcgtc acatcgtatc ttggcgacgt attgagctac agccgtctgc ttgcactagg
     5641 gcttgcaact tctgcggtag gcgtaattat caacatgctc gccggtcttg cgggggacgt
     5701 gccctatatt ggctggatca tcgcactcct tcttctcata ggagggcaca ttttcagtat
     5761 agctgtcaac gtactgggcg cttttgttca ctctctgcgt ctccagtatg tcgagttttt
     5821 tagcaaattc tatacaggag ggggaacagt cttttctcct ctgacgtata agacagcatt
     5881 tgtcgcaata aaaaatgaac ccgaagaata aactgttcag atttttcacg aatacgagag
     5941 gagtgtgtct ataactatgg atcttcttgg aatcatgttg gcaatacttg gagcggcaat
     6001 ggcggctgga tgggctggtg caggttcagc aatcggtgtt ggaatcgcag gagaagcagg
     6061 agcaggagtt atgacggagg accccggtaa atttggtctc gtgcttcttc tccaggctct
     6121 tcccggcact cagggcatct acggtcttct tatcgccttc ttcgccattc ttaaagttgg
     6181 tcttcttggc ggcggcgctg gagttaccgt gaacgtatgg cagggactcg gcattttctt
     6241 tgcctgcctt cccattgctg tcggcgg
//
LOCUS       DAOSWW010000032         6123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8456_length_6123_cov_4.237640, whole genome shotgun sequence.
ACCESSION   DAOSWW010000032 DAOSWW010000000
VERSION     DAOSWW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8456_length_6123_cov_4.237640"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PK442_01305"
     CDS             <1..982
                     /locus_tag="PK442_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86312.1"
                     /translation="SPASDVARLWSSGGSDSPVLHSSIFSAVSRGDVFLPALSSSGIE
                     GILHVGERRSRSPYRWNEVQLLFAAARMAGTFLERSRLGESARQARTLKEGDKLKSAI
                     FSSVSHELKTPLSSLTATISGLLEQDAEPDPSRVRQELLSVKKDLDRLSHSIGSLLEL
                     SRLAAEAWKPDLQWAEPGEVLSWVLFRLDKTDRERVRVDLPEHLPLILVDFQQWGRLL
                     MHLLENALSYSPAGSPVAVGARSTDEGFVTWIADEGPGIPDEEKSRVFEKFFRGTFSN
                     RLPGGTGLGLPIAMEIARVHEGSISIEDNTPAGTRIVVRLPAAHVMETEE"
     gene            987..1688
                     /locus_tag="PK442_01310"
     CDS             987..1688
                     /locus_tag="PK442_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO86313.1"
                     /translation="MTSAPERNIRVLVIDDEESIRRALKSVLSARKYEVSLAATAAEG
                     IDTAIETNPELVVLDLALPDMDGQDVCKELRAWMTAPILILSVRGNETDKIQALDAGA
                     DDYLTKPFQAGELLARMRALLRRAESRVVSPPELCAGDLTVNLARRQVFVKGEEVSLT
                     RTEFEILALLVQNADCVVTHRMLMEKLWDDKEERDPRLLRVHVSNLRKKIEPAPSLPR
                     FIHTEPGVGFRFTPE"
     gene            1809..2291
                     /locus_tag="PK442_01315"
     CDS             1809..2291
                     /locus_tag="PK442_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86314.1"
                     /translation="MTAQDIGLCSVIEKLRRNGAEADIAEAAQYMHRNEITLLMLKRA
                     GVPLEIMDACFLLSQCADSLIPERVEELRRSSLARTIKIGELRALLQEGDMRAAFRDR
                     WEICRESLVHALDILENGARADESPKKKPTMALFWERFAEFYEETEEMQNVALSGRSS
                     "
     gene            2263..2451
                     /locus_tag="PK442_01320"
     CDS             2263..2451
                     /locus_tag="PK442_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86315.1"
                     /translation="MLLCLGDLLEANASCSLEENGELHLYFQDESDILYGEDNKVVVS
                     AGESDLSGSDAVKWLQKH"
     gene            2585..3853
                     /locus_tag="PK442_01325"
     CDS             2585..3853
                     /locus_tag="PK442_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOO86316.1"
                     /translation="MLLIGAGTLLLLLFNIHSHPLRFVDALFTATSAVCVTGLVVVDT
                     GKDLAPASQTVVLLLIQLGGIGVMTATTALSLLIRERIGLRQRMFLAGGFGMETPAGV
                     VRFLRRILLLTFSVETVGALFLYAGFLRAGFSPLRSLYNAVFHSVSAFCNAGFSLFSD
                     SLAGFSRNLLIPGTVMALIVVGGLGFMVLSELMMTVRRREKLSMLPRLVLFTTAGLLL
                     FGLFSITLSEWNHAFAGMSGGWKIWNGLFASVTPRTAGFDTVPPATFSSAGIFLLILL
                     MIVGASPGSTGGGFKTTTLAVLLVSTWNNLRKNPEINLWNRRISIRILQRTVTVVVLY
                     LGTIGLGLGALALLEPFPFRSLLFETVSAMGTVGLSLGITAELSDAGKIVLMLLMFWG
                     RVGIVTFLYGLLKPVPRGKVSYPDADIPIG"
     gene            3866..4525
                     /locus_tag="PK442_01330"
     CDS             3866..4525
                     /locus_tag="PK442_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOO86317.1"
                     /translation="MNDKEKVVLVAGLGRFGQSLCKTLSEQGRQVIAVDRSRQFVEEM
                     ADIVDLAVQADVTDSEALVKVGAKEADIGIVAIGGNIEASILATTILKDLEIPYVIAR
                     AQNTVHARVLARVGANRVVFPERDMGERLALLLVHPWMSHFVQVPGSRFHVGEIRPLP
                     EMVGKTLPELNFRVRYNAVVLSVNRSGDRFLPTAETVIEAGDMLLVAGRREDLEKWVE
                     E"
     gene            4762..5628
                     /locus_tag="PK442_01335"
     CDS             4762..5628
                     /locus_tag="PK442_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86318.1"
                     /translation="MTSTALRPEDTSASAGALLFYYPAAFLLFFADILIVDFPGIRIG
                     MLDVLPAVPGITWSCVGAVFAVLMLVATVRYVAGGGTSDLARLFLRAGHSVVVLLSFI
                     GFLVVPMAVTLSGLSESTDTYGAFVAFRGAFFFLFLFVLSGMSHAFLATGRTDRGTTI
                     VGASQKLLAAQMAMLSAAFLFLVLSAPGTLPSLSCSMDVPSWLVYLLPVCFVLVGALF
                     ASFGGGAEHGALLGFMFRLCVLLSAAAPVAVLLAGRSEESGMALYMASLALFVSALKG
                     LYAVEASEEAAA"
     gene            5786..>6123
                     /locus_tag="PK442_01340"
     CDS             5786..>6123
                     /locus_tag="PK442_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO86319.1"
                     /translation="MQKQDTPFPVLFTPRLRLCAIESSHAERLHAVWTDPLVIEYLVL
                     DPFTELRQSEEMIGLLQWLHPSGEGVRWAITLRSDGTVLGTCGFHNWKKEHGRAEMGY
                     ELDSRMWRQG"
BASE COUNT         1112 a         1747 c         1889 g         1375 t
ORIGIN      
        1 gtctcccgcg tcggatgtcg cccgtttgtg gagttcgggg gggagcgaca gtccggtgtt
       61 gcattcgtcg attttctccg ccgtgtcgag aggggatgtg tttctcccgg cgctctcttc
      121 ttcaggaatc gagggcatac ttcacgtcgg ggaacgtcgc agccggagtc cgtaccgctg
      181 gaatgaagtg cagctcctct tcgccgccgc gcgcatggcc gggaccttcc ttgaacggag
      241 ccgcctcggc gagtcggcgc ggcaggcccg gaccctgaag gagggagaca aattgaagtc
      301 ggcgatattt tcgtcggttt cgcacgagtt gaagacgccg ctctcctcgc tgacggccac
      361 gatctcggga cttctggagc aggatgccga accggaccct tcgcgcgtac gccaggagct
      421 gctctcggtc aaaaaggatc tcgacaggct ctcgcactcc ataggctccc tgctggaact
      481 ttctcgtctt gccgccgagg cgtggaagcc ggatctgcaa tgggcggagc ccggcgaggt
      541 gctgagctgg gtgctcttca gactcgacaa gacggatcgc gagcgcgtac gggtcgacct
      601 cccggaacat ttgccgctta tccttgtcga ttttcagcag tggggaaggc tgctgatgca
      661 cctgctggag aacgcgctct cctacagtcc cgccggaagt cccgtggccg tgggcgcccg
      721 cagcacggac gaagggttcg tcacgtggat tgcggacgag ggacccggaa taccggacga
      781 ggagaagagc cgggtcttcg agaagttctt ccggggaacg ttttcgaacc gccttcccgg
      841 cggaacgggg ctcggtctcc ctatagcgat ggagattgcc agggtccacg aaggaagcat
      901 cagtatcgag gacaatacgc ccgcgggaac ccgtatcgtc gtccggctcc ccgcggcgca
      961 cgttatggaa acggaggaat gaacggatga cgagcgcgcc cgaaagaaat atcagggttc
     1021 tggtgatcga cgatgaagag tccatcaggc gggcgctcaa gtcggttctc tccgccagaa
     1081 agtacgaggt gagcctcgcg gcaaccgccg ccgaagggat cgacacggcc atcgagacga
     1141 accccgagtt ggtcgtcctc gaccttgcgc tcccggatat ggacggacag gatgtgtgca
     1201 aggagctgcg cgcctggatg accgctccca tcctcatcct ttcagtgcgg ggaaacgaaa
     1261 ccgacaagat acaggccctg gacgcgggcg ccgacgacta cctgaccaag ccgttccagg
     1321 ccggcgagct tctggcgagg atgcgggcgc tgttgcggag ggctgagtcg agggtcgtgt
     1381 cccctcctga actctgcgcc ggagatctga ccgtgaatct cgccaggagg caggtcttcg
     1441 tgaaggggga ggaggtttcc ctgacgcgca cggagttcga aatactggcg cttcttgtcc
     1501 agaacgccga ctgcgttgtt acccaccgta tgctgatgga gaagctttgg gacgacaaag
     1561 aagagcgcga cccgcggctt cttcgtgtgc atgtgagcaa cctgagaaaa aagatcgagc
     1621 ccgccccatc cttgccgcgc ttcattcaca ccgagcccgg agtaggattc cgcttcaccc
     1681 ccgaatgatc ttcacccttt acgaaaactt tacgcccgcg ccccgtttct tgacggagcc
     1741 tttatccctc gcttggtagc atatgcctgc acatggaaag aaatgccttt tcaacaggag
     1801 gattctttgt gactgctcaa gacatcgggt tgtgctccgt gatagaaaag ttgcggcgga
     1861 acggagcgga ggcggatatc gccgaggccg cgcagtacat gcatcggaac gagatcaccc
     1921 tgctcatgct gaagagagcg ggagtgccgc tcgaaatcat ggatgcatgt tttctgctga
     1981 gtcaatgcgc ggattcgctg attccggagc gggtcgaaga gttacgccgc agctccctgg
     2041 cacgaacaat caagatcgga gaactccgcg ccttgcttca agaaggggat atgcgcgctg
     2101 ccttccggga tcggtgggaa atatgcaggg aatcactcgt gcatgcgctg gatatactgg
     2161 agaacggcgc cagggcggac gaatccccga agaaaaaacc cacgatggcg ctgttttggg
     2221 agcggttcgc ggagttctac gaggaaacgg aggagatgca gaatgttgct ctgtctgggc
     2281 gatcttcttg aggcgaacgc ctcgtgttcg ctcgaggaaa acggcgaact gcatttgtac
     2341 tttcaggatg agtccgatat actgtacggc gaggacaaca aggtggtcgt atccgccggg
     2401 gagagcgatc tttccgggag cgacgccgtg aagtggttgc aaaaacactg attttgcgcg
     2461 tgcataagaa agaaaccgcg aagagagaaa tgcccgggaa gacgcacgtc cgggaggagg
     2521 tcgtagtata gcattcagag cctttacctc gctggccatt gaaaggaaac tcgtcgcagg
     2581 gttcctgctc ctgattggtg caggaactct gttattgctt cttttcaata tacattcgca
     2641 tcctctgcga tttgtggacg cgctctttac agctacttcg gcggtctgcg tgacgggact
     2701 cgtcgtcgtg gatacgggca aggacttggc gccggcctct cagaccgtcg tcctgctgct
     2761 catacagctt ggcggtatcg gtgtgatgac ggcgacgacg gcgctctccc tgctgatccg
     2821 ggagagaatc ggactacgac agcggatgtt cctcgccggt ggttttggta tggagacgcc
     2881 tgcgggcgtg gtgcgtttcc tgcggcggat tttgctgcta accttctccg tcgagactgt
     2941 cggggcgctc tttctctacg cggggtttct ccgtgccgga ttttccccgt tgcgttcgct
     3001 gtacaacgcc gtgtttcaca gtgtcagtgc cttctgcaac gccggcttct ccctcttctc
     3061 cgacagtctg gccggttttt cgcggaatct tctgattccg gggacggtca tggcgcttat
     3121 cgtcgtcggc ggactggggt tcatggtgct ttccgagctc atgatgacag ttcgcagaag
     3181 ggaaaaactt tcgatgctcc cgcgtcttgt gctctttacg acggcggggc ttttgctttt
     3241 cggccttttc tcaatcaccc tctcggagtg gaatcatgcg ttcgcaggga tgtccggcgg
     3301 atggaagatc tggaacggac tgttcgcgag cgtcacgccg aggaccgcgg gcttcgatac
     3361 ggtccctccg gcgaccttct cctccgccgg tatcttcctt ttgatactgc tgatgatcgt
     3421 cggcgcctcc cccggctcca caggaggcgg attcaagacg acgacgcttg cagtgctgct
     3481 ggtctcgacg tggaacaacc tcaggaaaaa ccccgagatc aacctgtgga acagacgaat
     3541 ctccatccgg atactgcaga ggaccgtcac ggtcgtcgtc ctctatctgg ggaccatcgg
     3601 attgggcctg ggggcgcttg cgctgctcga accctttccc ttcaggtcgc tgctcttcga
     3661 gacagtctcg gcgatgggaa cggtcggcct ttcccttgga atcactgcgg agctgagcga
     3721 cgcgggcaag atcgtgctca tgctcctgat gttctggggg cgcgtcggca tcgttacctt
     3781 cctctacggg cttctgaaac ccgtgccgag aggaaaggta tcctaccccg atgcggacat
     3841 accaataggt taggaggtgc gggaagtgaa cgataaagag aaagtcgtcc tggtcgcagg
     3901 gctgggacgt tttggtcagt cgctctgcaa gacgctttcc gagcagggga gacaggtgat
     3961 cgcggtggac cgttcccggc agttcgtcga ggagatggcg gacatcgtcg accttgccgt
     4021 gcaggcggac gttacggatt ccgaggcgtt ggtgaaggtc ggcgcgaagg aggctgacat
     4081 cggcattgtg gcgatcggcg gcaacatcga ggccagtatc ctcgcgacga ccatactgaa
     4141 ggatctggag atcccgtacg tcatcgcccg ggcgcagaat acagtgcatg cgcgtgtttt
     4201 ggcccgcgtg ggggcgaacc gcgtcgtctt ccccgagcgg gatatggggg agcggctggc
     4261 gttgctgctg gtgcacccgt ggatgtcgca cttcgtacag gtgccggggt cgcgttttca
     4321 cgtcggagag atccgccctc ttcccgagat ggtgggaaag accctgccgg aactcaactt
     4381 cagagtgcgc tataatgcgg tggtattgag cgtcaaccgg tccggcgacc gcttcctccc
     4441 cactgcggaa acagttatcg aggcgggcga tatgcttttg gtcgccggcc ggcgtgaaga
     4501 tctggagaag tgggtcgaag agtgaagcgc cctttcctcc tgttcatcgc cctaccctga
     4561 cgggcagttt tccgcgcacc cggaatgctg cggagcgaac gcctgctctc cggaagaccg
     4621 cttccggcaa gaaggccatt cacgaaacaa ataatggagc gccttcttgt accgatgttg
     4681 atttttaagc ttttttctgt ataatcagag cggcagtaaa tgccaaggcc acacttattt
     4741 ttatttcgag gaggttctcc aatgacaagc acagcacttc gtccagaaga cacgtccgct
     4801 tcagccggcg cactcctctt ctactatccg gcagcctttc tccttttctt cgccgatatt
     4861 ctgatcgtcg atttcccggg aatccgcatc ggcatgctcg acgttcttcc ggcagttccc
     4921 ggtattacgt ggtcgtgcgt cggcgcggtc ttcgccgttc tcatgctcgt tgcgacggtg
     4981 cgctacgtag cgggaggcgg aacgtcggat ctggcgcggc tcttccttcg ggccggacac
     5041 tccgtcgtcg tcctgctttc gttcatcggt ttccttgttg tgccgatggc cgtcacactt
     5101 tccggactct ccgaaagcac tgacacgtac ggcgcgttcg tcgcgttccg cggggccttt
     5161 ttcttcctgt tcctttttgt cctctccggg atgagccatg cgttcctggc aaccggacgg
     5221 accgatagag gcacgacgat cgtcggcgca agtcaaaaac tcctggctgc gcagatggcc
     5281 atgctttcgg cagccttcct tttccttgtt ctgagcgctc ccgggacgct gccctctctc
     5341 tcctgctcca tggatgtgcc ttcatggctt gtctaccttc ttcctgtctg ctttgtcctg
     5401 gtcggggcac ttttcgcatc gttcggggga ggagccgagc acggagcgct tcttggcttc
     5461 atgttccgcc tgtgcgttct tctctccgcg gccgctcctg tagctgtact gctggccgga
     5521 aggtctgaag agtcggggat ggcgctctac atggcgtcgc tcgcgctctt tgtatcggcg
     5581 ctcaaaggac tctatgccgt ggaggcctcc gaagaggctg ccgcatagca aaagctttct
     5641 gctccagtca taagggcgga ccgggttttt tccggttcgc ccttttccct tttctctacg
     5701 ccgttttgag tgtgtctcgc atcaaggact ttctttacca gctgctcgtc cgttgatgtg
     5761 cgggcgcgat atggaggaaa acgcaatgca aaaacaggat acccctttcc ccgtgctttt
     5821 cacgccccgg ctgcgactgt gcgcgatcga atcgtcccat gccgagcggc tgcacgcagt
     5881 ctggaccgat ccgctcgtca tagaatacct cgttctggat ccttttaccg agctgcgtca
     5941 gagcgaggag atgattggcc ttctccagtg gctgcacccc tccggagagg gcgtccgctg
     6001 ggcgatcacg ctgcgctccg atggaacggt gctcggaacg tgcggcttcc acaactggaa
     6061 gaaggaacat gggcgcgcag agatggggta cgaactcgac tcccgtatgt ggcggcaggg
     6121 ctt
//
LOCUS       DAOSWW010000033         6053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8600_length_6053_cov_3.485829, whole genome shotgun sequence.
ACCESSION   DAOSWW010000033 DAOSWW010000000
VERSION     DAOSWW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8600_length_6053_cov_3.485829"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="PK442_01345"
     CDS             <1..1459
                     /locus_tag="PK442_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HOO86320.1"
                     /translation="GHGGFPLEELAPFQYLRTDGIFGVPLGVSASFIFLFVLFGAVLS
                     VSGAGQFFIDLSLALTGRSQGGPGKAAVVASGLMGTVSGSSVANAVTTGAFTIPLMKE
                     AGYSATFSGAVVAASSTGGQVMPPVMGAAAFIMAQFLGISYWDIVVAAALPATLYFIS
                     IIAMVHFRAGRRGIKSLTEETAPKMGKVLKEGWHLLLPIITLVVFLAYGYSAVKAVFW
                     SIIIMVGVSWLGKAQYRMTPKRVLDALISGGMGAVEVAAACACSGLVIGVIGITGVGL
                     AFSSFVISLSGGVLPLALFLTMIGSIILGMGVPTTAQYIITSTLAAPALFQMGVPLMS
                     AHLFCLYFGVLADVTPPVALATYAAAGIAKSNAIKTGFTALVTAMAGFLVPYMFVYNN
                     HLLFQGNLLQIAFSFGTALMAIIGLAAGVQGYYFSVLSIFERVLLLIVPFLLIEPNII
                     TDVIGLVILVSVYLLQRRKAAKHAKEKTVSDAAQS"
     gene            1478..2185
                     /locus_tag="PK442_01350"
     CDS             1478..2185
                     /locus_tag="PK442_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOO86321.1"
                     /translation="MAHKLIKPVMSAEEAVTHIQPGSSLMVGGFNYAGVPYTLIDALV
                     AQGTDNLHLISNDTLYANDRHPEGIGHGKLVVNGQCRKVTASHVGLNRETQRLFNEGK
                     MELELVPQGSFVERIRCGGFGLGGFLTPTGVGTIHEEGKQVIEVDGKKFILELPLKAD
                     VAIIRGYKADRWGNLTYYGTGRNFNPAMATAADLVIAEVDMILAQGDIDPNDVVTPGM
                     LVDILVLKGDGYYASRT"
     gene            2169..2843
                     /locus_tag="PK442_01355"
     CDS             2169..2843
                     /locus_tag="PK442_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOO86322.1"
                     /translation="MLPVLDEEVVRHRIAQRIALDFEDGAVVNLGIGIPTLVSDYLPE
                     GVHVMLQTENGVIGAGPKPEVDDLRLIGAGGRCLSLLPGSSIVASDMSFGLIRGGHLD
                     ATVLGALEVDSHSNLANWMIPGKMVPGMGGAMDLVTGARAVYVATTHCDKKGRPKLVK
                     ECTLPLTGANVVSVVVTEFCLIRKIDGKMVLCEIAPEVTPEELKANTTMEYDVAPDLA
                     PMKGIE"
     gene            2869..4050
                     /locus_tag="PK442_01360"
     CDS             2869..4050
                     /locus_tag="PK442_01360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOO86323.1"
                     /translation="MGKPVLLSACRTPGGKFGGALKDLAAPDLGAIALAEAVKRSGVS
                     PEDIGEVIMGNGWQAGIGANPARIAMFRAGLPQSIPAFTINKRCGSSLKTAMLAADKI
                     RLGDMVAVAAGGMESASNVPYILPEARWGYRMGEKKALDALHKDGFHCPLSDMLMGAT
                     AEVLVEERSITREEQDEYALNSHRKAVAAIEGGFFAEEIVPVPVKDRKKGTVMVDTDE
                     IPRSDTSMESLGRLPALFKKDGTITAGSSSALCDAGSAYIVADSDWAAANGFTPLAEI
                     LGYASGALDALHMGLGPVVAMPKALQHAGMSLEDMEIIEINEAFSAQILACHRDMPFD
                     MEKLNPCGGAIALGHPIGATGGKILATLIYSLKRQKKEFGIVSACIGGGQGVAMVLRN
                     L"
     gene            4321..4566
                     /locus_tag="PK442_01365"
     CDS             4321..4566
                     /locus_tag="PK442_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86324.1"
                     /translation="MSRAFIKENEDQESYLEWQKLLRDREELLRILEKKKKYLLEDPA
                     AGTIPEEKRHEMIAKYDEEAEEVRRLLDEMLAETKIP"
     gene            4725..5636
                     /locus_tag="PK442_01370"
     CDS             4725..5636
                     /locus_tag="PK442_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO86325.1"
                     /translation="MDRREFLKRSLAAGVGGAALLVPGSVKRVFADSGKLPGLAAVRG
                     GEPGEMFDRGIAALGGMERFVKKGQTVAIKPNMSWDVAPELAANTNPDLVERIVARCL
                     EAGASKVYAFDHTCDLWRNCYANSGIEAAVKRAGGIVSPAESASSYTKVSIPGARVLK
                     ETSIHRLLLDCDVFINVPVLKHHGGAGITMGMKNLMGTVWNRGEFHSKGLHQCIADVS
                     LLRRPDLTVIDAYRMLTRNGPRGTSASDVTVLKAQILSTDIVAGDCAGARIFGLDPKN
                     VGHIRMGAELGAGTMDLDSLGVARIVL"
     gene            5636..6037
                     /locus_tag="PK442_01375"
     CDS             5636..6037
                     /locus_tag="PK442_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO86326.1"
                     /translation="MDRKRAGAAGKLRSLFALAVLLLMTMFFLFFAGVREGGLPWLQA
                     VPSVLRLWTGALILAASAALAGRVYCSVLCPLGTLQELIWRAGKRSASYRKPSQLSYW
                     VLGGVVVAAAAGYMFPAAFLDPYAAFGRGVM"
BASE COUNT         1190 a         1630 c         1957 g         1276 t
ORIGIN      
        1 cggacacgga ggctttccct tggaagagct tgctcccttc cagtatctgc gcaccgacgg
       61 tatcttcggg gtgccgctcg gcgtctcggc ttcgttcatc tttctcttcg ttctcttcgg
      121 ggctgtcctg agcgtttccg gcgcggggca gttctttatt gacctttcgc ttgccctgac
      181 agggcgcagt cagggagggc ctggcaaagc agccgtcgtg gcgagcggcc tgatgggaac
      241 ggtttccgga agctccgttg caaatgccgt aacaacaggc gccttcacca ttccgttgat
      301 gaaggaggcc ggatacagcg cgacgttctc gggagccgtc gtggcggcgt cctccacggg
      361 cggccaggtc atgccgccgg tcatgggggc tgcggcgttt atcatggcgc agtttctggg
      421 tatttcctac tgggatatcg tcgttgctgc tgcgcttccc gcaacgctct atttcatctc
      481 catcatcgcg atggtccatt ttcgtgccgg aaggcgaggg atcaagagtc tcacagaaga
      541 gaccgctccc aagatgggga aggtgctgaa ggaagggtgg catcttctgc tgccgatcat
      601 caccctcgtc gtctttctcg catacggcta ttccgcggtg aaagcggtct tctggtcgat
      661 catcatcatg gtcggcgttt cctggctcgg caaggcgcag taccgcatga cgccgaagcg
      721 tgttctcgac gctctgatca gcggcggcat gggggcggtg gaagttgcgg ccgcgtgcgc
      781 atgttcgggg cttgtcatcg gcgtcatcgg tatcaccgga gtcgggcttg cgttctcctc
      841 ctttgttatc agcctctccg gcggcgtgct tccgctcgct ctcttcctca cgatgatcgg
      901 ctcgatcatt cttggcatgg gcgttcccac cacggcgcag tacatcatca catcgacgct
      961 ggctgctccc gcgctttttc agatgggcgt tccgctcatg tcggcgcatc tcttctgtct
     1021 ctatttcggc gtgctcgcgg atgtaacgcc gccggtagcg ctggcgacct acgccgctgc
     1081 cggaatcgca aagagcaacg cgatcaagac cggattcacc gcgctggtta cggcaatggc
     1141 aggattcctc gtcccttaca tgttcgttta taataaccac ctgctcttcc aggggaacct
     1201 gctgcaaatc gcgttctcct tcggaacggc gctcatggca atcatcggtc tcgcggcggg
     1261 tgtccagggg tactacttct cggtcctctc aattttcgag agagtgcttc tgcttattgt
     1321 tcccttcctg ctcatcgaac cgaatatcat cacagacgtg atcgggctcg tcattcttgt
     1381 gagcgtctat ctactgcaaa gacgcaaagc ggcgaagcac gcgaaagaaa aaactgtttc
     1441 agacgctgca caatcttaaa ggaggtgtca tctcttcatg gcgcataagc tcatcaagcc
     1501 cgtcatgtcc gccgaggagg ccgtgacgca catacagccc ggttcctcgc tgatggtggg
     1561 aggattcaac tatgcggggg ttccctatac cctgatcgac gcgctcgtgg cgcaggggac
     1621 ggacaacctg catcttattt cgaacgatac tctctacgcc aacgaccggc acccggaagg
     1681 gataggccac ggcaagcttg ttgtgaacgg tcagtgcaga aaagtgaccg cgtcgcacgt
     1741 cgggttgaac agggaaacgc agagactctt caacgaggga aaaatggagc tcgaactggt
     1801 tccgcagggg tctttcgtgg aacggatccg ctgcggaggg ttcggcctcg gcgggtttct
     1861 gacgccgacc ggcgtcggga cgattcacga ggaggggaag caggtcatcg aggtcgacgg
     1921 aaaaaagttt attctcgaac ttcccctgaa ggcggacgtg gcgatcatcc gggggtacaa
     1981 ggcggaccgc tggggaaacc tgacctatta cggcacggga cggaacttca acccggcgat
     2041 ggccacggcg gccgatctgg tgatcgcgga ggtcgatatg atcctggctc agggagatat
     2101 cgacccgaac gacgtcgtca cccccggaat gctcgtcgat atactcgttc tgaaaggaga
     2161 cggatactat gcttcccgta cttgatgaag aagttgtccg ccatagaata gcacaacgga
     2221 tcgcgctcga tttcgaggac ggcgctgtgg tgaacctcgg catcggcatt ccgacgctgg
     2281 tttcagatta ccttcccgag ggtgtgcacg taatgctgca gacggagaac ggcgtgatcg
     2341 gggcagggcc gaaaccggag gtggacgacc ttcgtcttat cggggcggga gggcgctgtc
     2401 tttcgcttct tcccggttcg tccatcgttg ccagtgatat gagcttcggg ctcatcaggg
     2461 gaggtcatct cgacgcgacg gtgctcggcg ccctcgaggt ggacagtcat tcgaatctcg
     2521 ccaactggat gatccccggc aaaatggttc ccggcatggg cggggcgatg gatctggtga
     2581 ccggcgccag agcggtgtac gtcgccacga cgcactgcga caagaagggg cgcccgaagc
     2641 tcgtgaagga gtgcactttg ccgcttaccg gagcaaacgt ggtcagcgtc gtcgtcaccg
     2701 agttttgtct tatacgtaag atcgacggga agatggtgct ctgtgaaatt gcgccggaag
     2761 tcactcccga ggagctgaag gcgaacacaa cgatggagta cgacgtcgct ccggatctcg
     2821 cgcctatgaa gggcatcgag tagcgaatac gaaaggacag gaagaaaaat gggcaaaccg
     2881 gtactattaa gcgcgtgcag aacacccggc gggaaattcg gcggggcgct caaggatctt
     2941 gccgcccccg acctgggagc gattgctctt gccgaggcgg tgaagcggtc gggcgtatct
     3001 ccggaggata tcggcgaggt catcatggga aacggctggc aggcgggcat cggggcgaat
     3061 ccggcgcgta tcgcgatgtt ccgcgccgga ctccctcagt ccattcctgc gttcacgatc
     3121 aacaagcgct gcggctccag cctcaagacg gcgatgctcg ctgcggacaa gattcgcctc
     3181 ggggacatgg tcgccgtggc tgcgggcggt atggagagcg cttccaacgt gccgtacatt
     3241 cttccggagg cccgatgggg gtaccggatg ggcgagaaga aggcgctcga cgcgctccac
     3301 aaggacggat tccactgccc tctttcggat atgctcatgg gggcgacggc ggaggtgctc
     3361 gtcgaggagc gctccatcac cagggaagaa caggacgaat acgcgctcaa cagccacagg
     3421 aaggccgtcg cggcaataga aggcggtttc ttcgccgagg agatcgttcc cgttccggtg
     3481 aaggacagga agaaggggac cgtcatggtc gataccgacg agattcccag aagcgatacg
     3541 tcgatggaat cgttggggag gctgcccgcg ctctttaaaa aggacgggac gattacggcg
     3601 ggttccagct ccgccctgtg cgacgccgga agcgcgtata tagtagccga cagcgattgg
     3661 gctgcggcga acggcttcac acctttggcg gagatactcg gatacgcttc gggcgcgctc
     3721 gatgcccttc atatggggct cgggcctgtc gtggccatgc cgaaagcgct tcaacacgcc
     3781 ggaatgtcgc ttgaggatat ggagatcatc gagatcaacg aggccttctc cgcgcagatc
     3841 ctcgcatgcc acagggatat gccgttcgac atggagaagc tgaatccctg cgggggcgcg
     3901 atcgcgcttg gtcacccgat aggggcgacc ggaggaaaga tcctcgcgac gctgatctac
     3961 tcgctgaaac gccaaaagaa ggagttcggg atcgtcagcg cgtgtatcgg cggcggccag
     4021 ggagtggcga tggtgcttcg gaatctgtaa gtacgccaga gttccggcac tcttcgaaga
     4081 aagcgcgcgg gccgtttgtg tacgccggtc ccgcgcgctt tttattccac gcttcgcgat
     4141 cgtcggtacg agcgggcgct tcggatgcgg aggcgcttgc aaaagtttcg cggttgggcg
     4201 ataatagaat cgaaaacgca gttttttctg tcgtcatcat gaagagccaa cagggccccg
     4261 gatcctttcc gtggccttgt gtattgttgc gcttttcatc gtttgagagg ggggatagct
     4321 gtgagcagag cgtttattaa agagaacgag gatcaggaga gctacttgga atggcagaag
     4381 cttcttcggg acagggaaga gctgctgcgt atccttgaga agaaaaagaa gtatcttttg
     4441 gaagatcctg ccgccgggac aatacccgaa gagaagcgcc atgagatgat cgcgaaatac
     4501 gatgaagaag ccgaggaggt tcggcgtctt ctggatgaga tgctggcgga gacgaagata
     4561 ccctgatttt tcgcctgaga gtcgctgcaa aaagccgtcg taagacaggg atatacattc
     4621 tccctgattt acgacggctt tttgcactct actcaggaat gtcgaataaa tgtacatcgc
     4681 gaacgtgcta ttatataaca agagctgttc gaggaggtgt gagaatggac cggagagagt
     4741 ttttgaagag gagccttgct gcaggggtag gaggcgcggc gctgctggtt ccgggaagcg
     4801 tgaagagggt ctttgcggac tctggaaagc tgcccggact ggcggccgtt cgcggaggag
     4861 aacccgggga gatgttcgat cgcggcatcg cggcccttgg aggaatggag cgttttgtga
     4921 agaaagggca gactgttgcg atcaagccga acatgtcctg ggacgtcgcg cccgagctgg
     4981 cggcgaacac gaacccggat ctcgtcgagc gcattgtggc ccgctgcctg gaggcgggag
     5041 cgtcgaaggt gtatgctttt gatcatacgt gcgacctgtg gcggaactgt tacgcgaaca
     5101 gcggcatcga agcggcggtg aaacgggccg gcggcatcgt ctccccggcg gagtccgcga
     5161 gctcttacac gaaggtctcc attcccggcg cgcgggtcct caaggagacc tcgattcacc
     5221 gtctgcttct cgactgcgac gtttttatca acgttcccgt gcttaaacat cacgggggcg
     5281 ccgggatcac gatggggatg aaaaacctta tgggaacggt atggaacagg ggagagtttc
     5341 actcgaaggg gctgcatcag tgcatagccg atgtgagcct tctccgcagg ccggacctta
     5401 cggtgatcga cgcgtacagg atgctcacgc ggaacgggcc gcgcgggacc tccgcgagcg
     5461 acgtgacggt actgaaggcg cagatcctct cgacggacat cgtggccgga gactgcgccg
     5521 gggcgaggat tttcggcctt gatccgaaaa atgtcgggca tattcgtatg ggcgcggagc
     5581 tcggcgcggg gacgatggat ctcgattcac tcggcgtagc gcgcatcgta ttgtgatgga
     5641 caggaagaga gcgggagcgg cggggaaact ccgctccctt tttgctctgg cggttcttct
     5701 tctcatgacg atgttctttc tcttcttcgc gggagtgaga gagggaggcc ttccgtggtt
     5761 gcaggccgtt ccgtcggtac ttcgattgtg gaccggagcc cttatccttg ctgcaagcgc
     5821 cgccctggcg gggcgcgtgt actgctccgt cctctgtcct ttgggaacgc tacaggagct
     5881 gatttggagg gcgggaaagc ggagtgcgtc ctacagaaaa ccatcgcagc tcagctactg
     5941 ggtgcttgga ggagttgtgg ttgcggctgc ggcggggtac atgttcccgg cggcgttcct
     6001 cgacccctat gccgcgttcg gcaggggagt gatgtaaatg cccttcccct ctg
//
LOCUS       DAOSWW010000034         6031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8646_length_6031_cov_3.778614, whole genome shotgun sequence.
ACCESSION   DAOSWW010000034 DAOSWW010000000
VERSION     DAOSWW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8646_length_6031_cov_3.778614"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PK442_01380"
     CDS             complement(<1..773)
                     /locus_tag="PK442_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOO86327.1"
                     /translation="MLENVWSTIDLFLDHLRFEKEASPHTVTNYAADLAQFAEFLDHQ
                     GVVRPESVDSRIVRFFLREIMGFGYSKTSVSRKLSAVRSWSAFLKMRGVLASDPAKGV
                     RGPKLPKLLPRALSREDAQLLVEDGPDGVDRVRDRAILELLYGCGLRIAELVSLRWEA
                     MEPVEERMLRVAGKGEKERIVPFGQCALAALREWRPFVPLDAPFLFPGLKGGAITVRT
                     VHRVVLRAAARVGLSGVTPHTLRHSFATHLLEGGATLAVV"
     gene            complement(937..2295)
                     /gene="trmFO"
                     /locus_tag="PK442_01385"
     CDS             complement(937..2295)
                     /gene="trmFO"
                     /locus_tag="PK442_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOO86328.1"
                     /translation="MNRRVTVVGGGLAGSEAAWQLARRGIEVLLLEMRPLRTTPAHTT
                     EMLAEVVCSNSFGADGAASPAGILKNELRRLDSLILSCADESRVPAGKALAVDREIFS
                     RKVTERLESLPQVAVRREECVSLPEGPVIVATGPLTSPALAAILQRQAGTEYLSFFDA
                     VAPVVVADSIDMSVAFRRGRWGQEEDYINCPFTRERYEAFWNALVSAERALPHDFEDS
                     PSWFEGCLPVEVMASRGIDTLRFGPLRPVGLPLPGTGEEAWAVVQLRQDNREGTLFNL
                     VGFQTSLRWGEQERVFRMIPGLENAEFARLGVMHRNIYVNAPATLDKRLRFREGRTDL
                     FLAGQITGVEGYMESTAMGCVAALNAAALVDGVPFPEWPRATSIGSLLHYLGDAEPKH
                     FQPMNMNLGIMPKPEGKIRNKALRCEYVAEAASKAFDSFLESLKADSQTDDCQSEISG
                     ER"
     gene            complement(2288..4729)
                     /gene="topA"
                     /locus_tag="PK442_01390"
     CDS             complement(2288..4729)
                     /gene="topA"
                     /locus_tag="PK442_01390"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOO86329.1"
                     /translation="MVRKKIVTEAEVVESPSAPPKKGGKTAAKAEQGTAAPVKKAAPK
                     SASKKTLSSSAAKPKKTASSKTSAAKASSEKTLSADALPKKPVKKQAAKKSPASRPKT
                     KTALSSAAGSSDRTLVIVESPTKAKTLTKILGSKYTVKSSVGHIRDLPKSRLAIDIEN
                     DFAPEYILVKGKAKLKNELLAAAKQSPRVLLASDPDREGEAIAWHLAELLDIPLDTEC
                     RVRFYEITPTAVKEALAHPERVNMHKVEAQQARRILDRLVGYTLSPLLWKKIRRGLSA
                     GRVQSVALALICEREREIEAFVPKEYWIVTVLAVAEDGRVYSLKVDSFDGKSLWKEGK
                     SLLIDSEEFVDVLVGEILKAPLTVSDFKVRESYRAAPAPFKTSTLQQESARRNSMSPR
                     RTMSIAQELFEGVSLPDRGPTGLITYMRTDSLRIAPEALETCRQYIASAFDPEYLPEK
                     ANEYASKGRSQDAHEAIRPTDVTIVPKSLEGILTNDQMKLYSLIWNRYVASQMSKAKV
                     ANATLAATAGKVGLRQLGETLVFPGWSAVWPLDLKGESFAPARAGETLRVEKTEREQK
                     FTRPPARYSEASLIKVLEDEGVGRPSTYASIVETLYDRGYVARNEERRLQPSELGKTV
                     DSFLRKYFDAESLSAIVDTGFTARMEESLDDVEESNISWLAVLKEFWDAFTRTMQDAE
                     KAPAEKLPPPEPTGEDCPECGKPLVLKNGRFGDFIACSGYPDCKFTKPVLVKTGAVCP
                     KCGEGDVVKRKSRKGKPFYGCSRYPACDFVSWNPPSGETCPQCGAALFAKGTKGAEEC
                     MKCGFTRVRETAADE"
     gene            complement(4745..5071)
                     /locus_tag="PK442_01395"
     CDS             complement(4745..5071)
                     /locus_tag="PK442_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86330.1"
                     /translation="MPIIKKICGTRGRITESLEFKRVLHDLERRYLSPDAQAKIYRYL
                     ATEEVPLEVTERAILEAVALARLKNATADASLFDFLVDALLDDCSFEIPETASERVYP
                     SSCTLC"
     gene            complement(5103..>6031)
                     /gene="dprA"
                     /locus_tag="PK442_01400"
     CDS             complement(5103..>6031)
                     /gene="dprA"
                     /locus_tag="PK442_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOO86331.1"
                     /translation="DSSWPDEEELRAKQAGVRLLTFRDKEWPEAFSGLPDSPRLLYVK
                     GKWPLRGPSVAVVGTRRCSAYGMRAAFEIGRAVGGAGGVVISGGAAGIDKAAHEGCLS
                     AGGASAAVLGTGVDVAYPRGHEPLFVRLAEEGGALLSEYPLGSQPKQWRFPERNRIIA
                     SLADRLVVVEAPSKSGAMITARCALEAGREVWAVPGRINESGCEGSNRLLYDGAVPLV
                     SIPDFISLALGRQLSLFEETPFRGEQNSVAFTEKEQRILCLLEKSGERTVDNLAVEGT
                     MAPADVFSCLAALMAAGRVFVAGPGRWSAVPK"
BASE COUNT         1149 a         1892 c         1827 g         1163 t
ORIGIN      
        1 acgaccgcaa gcgtcgcgcc tccctcaagg agatgcgtgg caaagctgtg ccgcagcgtg
       61 tgcggcgtca caccgctcag gcccacccga gccgccgcgc ggagcacaac cctgtgcacc
      121 gtccggacgg tgatggcgcc gcccttcaag ccgggaaaga gaaacggcgc atcgagcgga
      181 acgaacggac gccattcgcg aagcgcggcg agcgcgcact ggccgaacgg gacaatacgc
      241 tccttctctc ccttgcctgc aacgcgaagc attcgttcct cgaccggctc catggcctcc
      301 catcgaagcg agacaagctc tgcgatccga agaccgcaac cgtagagcag ctccaaaatc
      361 gccctgtcgc ggacccggtc gacgccatcc ggcccgtctt cgaccagaag ctgcgcgtcc
      421 tcgcgggaaa gcgccctggg caggagcttc ggaagcttcg gcccccgtac tcccttcgcg
      481 gggtcggaag ccagcacgcc gcgcattttc aagaacgccg accagcttcg cacagcggag
      541 agcttcctgg agacggaggt ttttgagtag ccgaacccca tgatctcccg aagaaaaaag
      601 cgtactatcc gggaatcaac gctctccggg cgcacaactc cctggtggtc aaggaactcc
      661 gcaaactgag ccagatccgc agcgtagttg gtcaccgtat gcggcgaggc ttctttttca
      721 aatcgcaggt gatcaagaaa aagatctatg gttgaccaga cgttttccaa catagagaca
      781 ttttatcacg ttttctagat tctcgctatt tttcaaaaga atcaattaag gcatggtttc
      841 ttttgtgcaa aaccgcatga acagtgcatt gagcacagaa aatgatctga agagcatatt
      901 tttatacaaa gagaattatg agaaaaaaca agtcgtttac cgctcaccgg aaatttcaga
      961 ctggcagtcg tccgtctgag aatccgcctt caatgattca agaaaagaat cgaaagcctt
     1021 ggatgcggcc tcggcgacgt attcgcaccg gagcgccttg ttccgaatct ttccctccgg
     1081 tttcggcatg atgccgaggt tcatgttcat cggctggaag tgcttcggct ccgcgtcacc
     1141 caggtagtgc agcagcgaac ctattgaggt cgccctcggc cactccggaa acggaacgcc
     1201 gtcgacaagc gccgccgcgt tgagcgccgc aacgcacccc atcgcggtgc tctccatgta
     1261 cccctcgacg ccggtgatct gtcccgcgag gaagagatcc gttctgcctt cgcggaagcg
     1321 cagacgcttg tcgagcgtcg ccggggcatt gacgtagatg ttcctgtgca taacgccaag
     1381 acgggcgaat tcggcgtttt caagcccggg gatcatccgg aagacgcgct cctgctcccc
     1441 ccaccggagg ctcgtctgaa agccgacgag attaaagagc gtcccttcac ggttgtcctg
     1501 acgaagctgc acgacagccc atgcctcttc ccccgtgccg ggcagaggaa gccccaccgg
     1561 ccgcagcgga ccgaagcgga gggtatcgat accgcgcgaa gccatcacct cgacggggag
     1621 acacccctcg aaccaggatg gagaatcctc gaagtcgtgc ggaagcgcgc gctctgcgga
     1681 gacgagcgca ttccagaacg cctcgtaccg ttcgcgcgta aaggggcagt tgatatagtc
     1741 ctcctcctgc ccccagcgtc cccgccggaa ggcgacggac atgtcgatag agtcggcgac
     1801 gacgacgggg gcgacggcgt cgaagaagga gaggtactcc gttccagcct gtcgctgcaa
     1861 gatcgcggca agcgccgggg atgtcagcgg ccccgtggcg acgatcaccg gtccttcggg
     1921 caaggagacg cactcctccc tgcgaacggc gacctgcgga agcgattcga gccgttccgt
     1981 gacctttcgc gagaagattt ccctgtcgac ggcgagcgcc ttgcccgcag gaacgcgaga
     2041 ttcgtcggcg caggagagga taaggctgtc cagacggcgc agctcgttct tgaggattcc
     2101 ggcgggactc gcggcgccgt ccgcgccgaa ggagttgctg cagacgactt cggcgagcat
     2161 ctccgtggtg tgcgccggag tcgtccgaag ggggcgcatt tcgagcagca atacctcaat
     2221 accgcgccgc gcaagctgcc acgcggcctc cgaaccggcg agtccgcctc cgacgacggt
     2281 gacgcgccta ttcatccgca gccgtctccc gtacccgggt aaaaccgcac ttcatgcact
     2341 cctccgcgcc cttcgttccc ttggcgaaaa gagccgcgcc gcactgcgga cacgtctccc
     2401 ccgagggggg attccaggag acgaagtcgc acgccgggta gcgcgaacag ccgtagaagg
     2461 gcttcccctt gcggctctta cgcttgacga catctccctc gccgcacttc ggacagaccg
     2521 ccccggtctt cacaaggacc ggtttggtga atttacagtc ggggtagccg ctgcatgcga
     2581 tgaaatcgcc gaaacggccg ttcttgagca cgagcggctt gccgcactcg ggacagtctt
     2641 cgccggtagg ttccgggggc ggaagctttt cggccggggc cttctccgcg tcctgcatcg
     2701 tgcgggtaaa ggcgtcccag aactctttga ggacggcgag ccaggagata ttgctctcct
     2761 cgacgtcgtc cagactctcc tccatgcggg cggtaaagcc tgtgtcgacg atcgcggaga
     2821 ggctctccgc gtcgaagtat ttcctaagga aggagtccac cgttttcccg agctcggagg
     2881 gctgaaggcg gcgctcttcg ttgcgcgcga cgtaccctct gtcgtagagc gtctccacga
     2941 tggatgcgta cgtcgaggga cggccgacgc cttcgtcctc gaggaccttg atcaggctgg
     3001 cctcggagta gcgtgcgggc gggcgcgtga atttctgttc gcgctccgtc ttctcgacgc
     3061 gaagcgtctc cccggcgcgg gccggagcga agctctcccc cttgaggtcg agcggccaga
     3121 cggcgctcca ccccggaaag acgagcgtct cgccgagctg gcgcaacccg accttgccgg
     3181 cggtcgcggc gagcgtcgcg ttggcaacct ttgccttcga catctgcgag gccacgtagc
     3241 ggttccagat gagcgagtag agcttcatct gatcgttggt gaggatgcct tcgagactct
     3301 tcggcacaat ggtcacatcg gtcggccgga tcgcctcgtg cgcgtcctgg gagcgtcctt
     3361 tggacgcgta ttcgttcgct ttttccggaa ggtactccgg atcgaaggcg gaggcgatgt
     3421 actgtctgca cgtctcaagc gcctcgggag cgatcctgag gctgtcggtg cgcatgtagg
     3481 tgatgaggcc ggtcggcccc ctgtcgggca gagagacgcc ttcgaaaagc tcttgggcga
     3541 tgctcattgt ccggcggggc gacatgctgt tcctgcgggc cgattcctgc tgtagggtgc
     3601 tcgttttaaa aggagccggg gcggcgcggt agctttcgcg caccttgaag tctgaaactg
     3661 tgaggggagc tttcaggatc tcgccgacga ggacgtccac aaactcctcc gagtcgatga
     3721 gcagggactt cccttccttc cagaggctct tcccgtcgaa gctgtccacc ttgagcgaat
     3781 agacccttcc gtcctcggca acagcgagca ctgtgacgat ccagtactcc ttgggaacaa
     3841 acgcttctat ctcgcgctcc cgctcgcaga taagggcgag cgcaacagac tggaccctcc
     3901 ctgcggagag ccccctgcgt atcttcttcc agagaagcgg actcagcgta taccccacca
     3961 gccggtcgag aatgcgccgc gcctgctgcg cctccacctt gtgcatattg acgcgctcgg
     4021 ggtgcgcaag cgcctctttc accgccgtgg gagtgatttc atagaaacgg acgcggcatt
     4081 ccgtgtcgag cgggatgtcg agcagttccg caagatgcca cgcgatcgcc tcgccttcgc
     4141 ggtcggggtc ggaggcgagc agaacgcgcg gagactgctt cgccgcggcg agcaactcgt
     4201 tcttcagctt cgccttcccc ttgacgagga tatactccgg ggcaaagtcg ttctcgatat
     4261 cgatggccag cctgctcttc ggcagatcgc gaatgtggcc gacgctcgac ttcaccgtgt
     4321 acttcgagcc aaggatcttc gtgagcgtct tcgccttcgt gggagactcc acgatgacga
     4381 gcgtcctgtc cgaagagcct gccgcggaag aaagcgctgt cttcgtcttc ggccgggaag
     4441 ccggactctt tttcgccgct tgttttttca cgggtttctt cgggagcgcg tccgctgaaa
     4501 gcgtcttctc ggaagacgct ttcgcagcgg aggtcttcga cgaggccgtc ttcttcggtt
     4561 tcgccgcaga agacgaaagt gtcttttttg aagcgctttt cggggcggcc ttcttcacgg
     4621 gcgccgcagt cccttgttca gccttcgcgg ccgtcttccc gcccttcttc ggcggagcgg
     4681 agggactctc tacaacctcc gcttcagtta cgatcttttt gcgaaccatg cgaaaaaacc
     4741 gtcctcagca aagggtgcag ctcgaaggat agaccctctc cgaggccgtc tcggggatct
     4801 cgaaggaaca gtcgtcgagc agcgcgtcta cgaggaaatc aaaaagagaa gcgtccgcag
     4861 tggcgttctt gagacgggca agagcgaccg cttcaagaat cgcccgctcc gtgacctcca
     4921 gcggcacttc ctctgtagca agatagcggt aaatcttcgc ctgtgcgtca ggggagagat
     4981 acctccgttc cagatcatgg agcactcttt tgaactcgag agactcagtg atgcgccccc
     5041 gggttccgca aatttttttt ataatgggca ccaggtttct tcctttcccg aatggtttgg
     5101 agttacttcg ggacggcgct ccaacggccc ggtcccgcta caaaaacacg tcccgcagcc
     5161 ataagcgcgg cgagacagga aaatacatca gcgggagcca ttgtaccctc aacagcaagg
     5221 ttgtcaacag ttctctcccc tgatttttcc aaaaggcaaa gaattctttg ctctttttca
     5281 gtaaaggcaa cggaattttg ctctccccgg aacggcgtct cttcgaagag ggagagctgg
     5341 cgtccgagcg cgagggaaat gaagtcgggt atcgacacca gcggtacggc gccgtcgtac
     5401 aggaggcggt tggaaccttc gcaccccgat tcgttgatcc ttcccgggac cgcccatact
     5461 tcccgtcccg cttcgagggc gcagcgggcg gtaatcatgg cgccgctctt cgaaggcgcc
     5521 tcgacgacga ccagacggtc ggcgagcgac gcaatgatgc ggttgcgctc cggaaagcgc
     5581 cactgcttgg gctgcgaacc gagggggtat tccgagagca gcgcgcctcc ctcttcggcg
     5641 aggcgaacaa agagcggttc gtggccgcga gggtaggcga cgtcgacgcc tgttccgagg
     5701 acggcggcgc ttgctccgcc agcggaaagg catccttcgt gggcagcctt gtcgatcccc
     5761 gccgctccgc cgctgatgac gacgccccct gcgccgccga cggcccgccc tatctcgaat
     5821 gcggcgcgca taccgtacgc cgagcatcgt ctggtcccca cgacggcaac tgaagggccg
     5881 cggagcggcc acttgccttt gacgtagagc agccgcgggg agtcgggcag tccgctgaac
     5941 gcttcgggcc actccttgtc gcggaaggtg agaagacgga cgccggcctg cttcgcgcgc
     6001 agttcctctt cgtcgggcca ggaagagtcc a
//
LOCUS       DAOSWW010000035         5975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8772_length_5975_cov_3.477027, whole genome shotgun sequence.
ACCESSION   DAOSWW010000035 DAOSWW010000000
VERSION     DAOSWW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8772_length_5975_cov_3.477027"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PK442_01405"
     CDS             complement(<1..368)
                     /locus_tag="PK442_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HOO86332.1"
                     /translation="MKTPSSTPENHYLSLDADWGEALARIPESSSKKVSGPFRGHFDG
                     ASRGNPGEAGAGALLLDASGTPVWTCARPLGRKTNNEAEYAALILLLEEILARGLAGV
                     SVRGDSQLVVNQVTGRWKVK"
     gene            543..1085
                     /locus_tag="PK442_01410"
     CDS             543..1085
                     /locus_tag="PK442_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86333.1"
                     /translation="MTLSPVLSFRRGGASAGSSPHCLKGLFLLLLLFFCFAPILSPLP
                     ADAKGPEEIVFLKGLFVKEVRGYGLYVEEESNRFHKDDKPTIYLEVDNFKLGVKDEQY
                     HISLSLDLLVKDAKGAVVAEDKQIITHEYFLKSRVRDVWFTVILDLSGWPEGKHILSW
                     VVTDNTSGKQCTKDMEIEIH"
     gene            1246..2541
                     /gene="larC"
                     /locus_tag="PK442_01415"
     CDS             1246..2541
                     /gene="larC"
                     /locus_tag="PK442_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOO86334.1"
                     /translation="MKTLYLDCFAGIAGDMFLGAMLDLGLDREAFLRTMESVVLFPGH
                     EHGHAHPDPHDHEHEHSHEHNDTHHHAHHHSHGTDRLRIAITQTVRGGIAGTKVNIAS
                     SEDHPHRGLADVLEIIGASPLSPRVKERSAAAFRLLAEAEAKVHGTTPDKVHFHEVGA
                     LDSIADIIGAFVLVEMAGVDTVVSSPLNVGHGTITCAHGILPVPAPATAELLAGLPVR
                     VEGGPMERVTPTGALLVRCLASSFGPLPAGKVLGSGKGAGDRDGDIPNVLRVLLLETE
                     ATNTDGAGNIARDSGIVIETNIDDMNPQYYAPVMNRLLAEGALDVWAAPIVMKKGRPA
                     ITLGCLCRPGAERSLAETLLRETTTLGVRMYAVDRLKADAEIVERETSWGIVRFKVAR
                     LGEEVLRSNPEFEDVRRLSEEHGIPLPEMRERLMNEFLL"
     gene            complement(2619..2795)
                     /locus_tag="PK442_01420"
     CDS             complement(2619..2795)
                     /locus_tag="PK442_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86335.1"
                     /translation="MRFLLLALVLIAACIPMLLGSERVRPCYKGCHGCGKCMKSFRGD
                     EESEERTPQPPANR"
     gene            2943..3173
                     /locus_tag="PK442_01425"
     CDS             2943..3173
                     /locus_tag="PK442_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOO86336.1"
                     /translation="MHCLATFDITSMAVLFERVCRKEGITAKIVPVPRSLSTSCGLAC
                     EFPCEEEERVRELCGKKHVDVTDFHSLGDDWT"
     gene            3290..4978
                     /locus_tag="PK442_01430"
     CDS             3290..4978
                     /locus_tag="PK442_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO86337.1"
                     /translation="MEKKKVLIIGGVACGAKTAARLARLAPEFDITILERGEHLSVAG
                     CGFPYFVGDVVKEYKDLVCTPLGIVRDANFFRNVKNIAVHTGHLATRIDREKKVVAAT
                     DTASGEERLFHYDELVLATGASPIRPPIPGNDLSRVFTLWTMPDAMAMKSAIDAGSVK
                     SAVIIGGGLIGMEVVEALVDRGISVSVADIQPTPLPALAGEDFGLRVAKVLRQKGVGF
                     YGGEKVVEIIGENGVVTGVRTDKRTLPADMVLLAVGIRPNTILAKEAGLELHPNGTIV
                     VDECLRTSDPAIYAGGDCVQSKNILTEKSVWQPMGSTANRQGRVIADNIAELPTRFAG
                     VVGTAILKLFHLTIGKTGLNEEQAHAAGFDAVSIVVEEPDRPHFMPGMGAMTIRLVAD
                     RKSRRIVGAQIMGGGVVDKRLDSIVAAISAGMTVDMLADADFAYAPPFATALDPTTHA
                     ANALRNKMDGLVKTYSAKELKALMEGANPPFLLDVRSPQELDLQGRIPYDFANIPLGQ
                     LRGRITEIPKDREIVTFCKISVRGWDAIATLRGNGYENVALLEGGIMAWPYDLR"
     gene            complement(5069..>5975)
                     /locus_tag="PK442_01435"
     CDS             complement(5069..>5975)
                     /locus_tag="PK442_01435"
                     /inference="COORDINATES: protein motif:HMM:NF037272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HOO86338.1"
                     /translation="IMEYGALSIGMMADFNENTWNTETHAFFYNGPRGANHAVNVVGW
                     DDGFPKENFATTPPGDGAWIVRNSWGEEFGEGGYFYMSYYDTSVDSGIAYILASPDLY
                     GIIHQHDPLGWVRSVSASETNPETGWMANIFTAGEDQTLRAVSFYAAAYGTMYEVFID
                     DVSGGVSGSGEALRTAEASGTLEQPGYHTIPLPRPVGVGEGTRFRVRVKLTTPGYEYP
                     LPVELPLEEYSDNATASPGESFYSGDGASWTDLTEDFAEANFCIKVFSTPGMSRSGGS
                     GGCSAASASPLLFALLAPLLLLRRR"
BASE COUNT         1189 a         1899 c         1782 g         1105 t
ORIGIN      
        1 tccttcacct tccagcgtcc ggtgacctga ttcaccacaa gctggctgtc cccccggacg
       61 gagacgcccg cgagcccgcg cgccagtatc tcctccagca gcaggatgag cgcggcgtac
      121 tccgcctcgt tgttggtctt ccgcccgagc ggacgggcgc acgtccatac aggcgttccg
      181 gacgcgtcga gcagcagcgc tcccgccccg gcctcgccgg gattgccccg ggaagcgccg
      241 tcgaaatgcc cccggaaggg gccggaaact tttttcgagg aggactccgg aatgcgggca
      301 agggcttcgc cccagtccgc gtcgaggctc agatagtgat tttcaggggt actcgaaggt
      361 gttttcatag gatgctcctt atcagcaaag gtaactttcg ttaattttcg agaacaaata
      421 agccttaaga ttcatttgca cgatgtattt tcgataaaca gtatatttcc ctagtccatg
      481 aataaaaaaa tctatataat gctttgggct tttcattttt atctcaagga ggctgcatga
      541 ctgtgacact atcgccagtc ctttctttcc ggagaggcgg cgcttccgca ggaagctcgc
      601 cgcactgcct taaaggtctt tttctccttc ttcttctgtt tttctgcttc gcgccgattc
      661 tctcgcctct tcccgccgac gccaagggtc ctgaagaaat cgtcttcctt aaggggcttt
      721 tcgtcaagga ggtccgcgga tacggcctct acgtcgaaga ggagagcaac cgctttcata
      781 aagacgacaa accgacgatc tacctcgagg tcgataactt caagctggga gtgaaggacg
      841 aacagtatca tatcagcctt tcgctcgatc tgctcgtaaa agacgcaaaa ggcgccgtcg
      901 tcgccgagga caagcaaatc atcacgcacg agtatttcct gaagagcagg gttcgcgacg
      961 tctggttcac ggtgatcctg gacctctcgg ggtggccgga ggggaagcat attctctcct
     1021 gggtggtcac cgacaacact tccgggaagc agtgcacaaa agacatggag atcgaaatcc
     1081 actgaccttt tctcgtgaac gagaacacag cgccgccgga tgtatccgtt cgcccggcgg
     1141 ctttttgcgt ggggacggcg cgccgttttt ctcacctccc gatgtattcc gtccggcaga
     1201 tgatacaata accgcgttgt gaactttgca cacggaggtc ttttgatgaa aacactctat
     1261 cttgactgtt tcgccggaat cgccggcgat atgttcctcg gcgcgatgct cgacctcggg
     1321 ctcgacagag aggcgtttct ccgcacgatg gaaagcgtcg tgctctttcc cggccacgag
     1381 cacggtcatg cgcaccccga cccgcacgat cacgaacacg agcatagcca cgaacacaac
     1441 gatacacacc atcatgcaca ccatcattcg cacggcacgg acaggctgcg catcgccatt
     1501 acgcagacgg tccgcggcgg catcgcggga acaaaggtga acatcgcctc gtccgaggat
     1561 catccgcacc gagggctcgc cgacgtcctc gaaatcatag gggcaagtcc gctctctccg
     1621 cgtgtgaagg agcggagcgc cgccgccttt cgtcttttgg ccgaggccga ggcgaaggtc
     1681 cacggaacga ctccggacaa ggtacacttc catgaagtcg gggcattgga ctcgatcgcc
     1741 gacatcatcg gcgcgttcgt cctcgtggag atggcgggcg tggacacagt cgtctcctcg
     1801 ccgctgaacg tcgggcacgg aaccatcaca tgcgcgcacg gcatccttcc cgtcccggct
     1861 ccggcgaccg cagagctgct tgccggtctt ccggtccgcg tcgaaggagg gccgatggag
     1921 cgcgtcaccc ccaccggagc gctgctcgtg cgatgcctcg cttcctcgtt cgggccgctc
     1981 cccgcgggga aggtgctcgg aagcggcaag ggggcgggcg accgcgacgg cgacatcccc
     2041 aacgtactgc gcgtgctgct gcttgagacc gaagcgacca acactgacgg cgcggggaat
     2101 atcgcgcggg attcgggaat cgtcatcgag acgaacatcg acgacatgaa cccgcagtat
     2161 tacgcgccgg ttatgaatag gctcctcgcg gagggggcgc tcgacgtatg ggccgcgccc
     2221 atcgtcatga aaaaaggacg ccccgcaata acgctcggct gcctctgccg gcccggcgcg
     2281 gagcggagct tggcggaaac gctgctgcgg gagacgacga cgctcggcgt ccggatgtac
     2341 gcggtcgaca ggctgaaggc cgacgcggaa atcgtggagc gggagacctc gtggggtatc
     2401 gtccgcttca aggtggcgcg cctcggagag gaagttcttc ggagcaaccc tgagttcgag
     2461 gacgtccgga gactttcgga ggaacacggc atcccgcttc cggagatgcg ggaacgattg
     2521 atgaacgagt tcctcctctg aaaagcggcg cgccgcattc ggaccggaga gagccgtctg
     2581 ttcaggagtc cggaacgccg tcgcgtccgc agcagtcgtc agcgattcgc gggaggctgc
     2641 ggtgtgcgct cctcgctctc ttcgtcgccg cggaaggact tcatgcactt tccgcagccg
     2701 tgacacccct tgtagcaggg gcggacccgc tccgatccca gcagcatcgg gatacaggcg
     2761 gcaatcagca ccagcgcgag cagaagaaaa cgcacacgac gtcacctccg ggaaagagtt
     2821 tttttcagcc catagtatac gctctttttg aaaggttgtg tttgcatgcg ctttgaacga
     2881 accaggacgc acgaaccggc catttgccgg cacattcctc atcccgaaag gaggcggtag
     2941 cgatgcactg tctggcgacc ttcgacatca cgagcatggc cgtcctcttc gagcgagtct
     3001 gccgcaaaga gggaattacc gccaaaattg tccccgtccc gcgcagcctt tccacaagct
     3061 gcggacttgc ctgcgaattc ccctgcgaag aggaagagcg cgtccgcgag ctctgcggga
     3121 aaaaacatgt ggacgtgacc gatttccatt ctttggggga cgactggaca taacttcctt
     3181 tttgaaatac catatagggg tagcagtata catcgcggcg tcagggcgtc cgctcgctga
     3241 acatacgtcc gccgggccgc gaatttccga aaggcagggg attgtggtca tggagaaaaa
     3301 gaaggtcctc atcatcggcg gcgtggcatg cggcgcgaag acggcggccc ggctcgcgcg
     3361 acttgcgccg gagtttgaca tcaccattct ggagcggggc gaacatctga gcgtcgccgg
     3421 atgcggcttc ccgtatttcg tcggcgacgt ggtgaaggag tacaaggatc ttgtctgcac
     3481 gccgctcggc atcgtccgcg acgcgaactt cttccgcaac gtgaagaata tcgccgtcca
     3541 cacgggacac ttggcgacgc gcatcgacag ggaaaagaag gtcgtcgccg ctaccgacac
     3601 agcctccgga gaggagcgtc tcttccacta cgacgagctc gtactcgcca ccggcgcctc
     3661 ccccatccgt cctcccattc ccggaaacga cctcagccgc gtcttcaccc tctggacgat
     3721 gcccgacgcg atggcgatga agtcggctat cgacgccgga agcgtcaagt ccgccgtcat
     3781 catcggcggg gggctcatcg gcatggaagt cgtcgaggcg cttgtggacc gcggcatctc
     3841 ggtctccgta gccgacatcc aacccacccc ccttccggcg ctagccggag aggacttcgg
     3901 tctccgcgtg gcgaaggtgc tgcgtcagaa gggcgtcggc ttctacggcg gcgagaaggt
     3961 cgtcgaaatc ataggcgaga acggggtcgt caccggcgtc agaaccgaca agcgcaccct
     4021 tcccgccgac atggttctgc tggccgtcgg cattcgcccg aacacgatac tcgccaaaga
     4081 agccgggctc gaactccacc ccaacgggac catcgtcgtc gacgaatgcc ttcgcacgag
     4141 cgacccggcc atctacgccg gaggcgactg cgttcagtcc aagaatatcc ttaccgagaa
     4201 gtccgtctgg cagccgatgg gctccaccgc gaaccggcaa ggacgcgtca tcgccgacaa
     4261 tatcgcggaa ctgcccaccc gtttcgccgg agtcgtcggc acggcgatcc tgaagctctt
     4321 ccacctcacc atcggaaaga ccggcctcaa cgaggagcag gcgcacgcgg cgggattcga
     4381 cgccgtgagt atcgtcgtcg aggagcccga ccggccgcac ttcatgccgg gcatgggcgc
     4441 gatgacgatc cgcctcgtgg ccgacagaaa gtcccgccgc atcgtcggcg cgcagatcat
     4501 gggcggcggc gtggtggaca agcggcttga ttccatcgtc gcggcgatct ccgccgggat
     4561 gacggtcgat atgctggcgg acgccgactt cgcctacgcc ccccccttcg ccaccgcgct
     4621 cgaccccacg acgcacgccg ccaacgcgct gcggaacaag atggacgggc tggtgaagac
     4681 ctattcggcg aaggagctga aggcgctcat ggagggggcg aaccctccgt tcctgctcga
     4741 cgttcgctcc ccgcaggagc tcgaccttca ggggcgcatc ccctacgact tcgcgaacat
     4801 cccgctggga cagcttcgcg gccgtataac ggaaatcccg aaggaccgcg agatcgtgac
     4861 gttctgcaag atctccgtgc gcggatggga cgccatcgcc acgcttcgcg ggaacggcta
     4921 cgagaacgtc gccctcctcg aaggcggcat catggcctgg ccgtacgatt tacggtagaa
     4981 aacccgagta tttcatgcaa agagaagatg cgggcccccg agaactccgg gaacccgcat
     5041 cttctttttg cgtaacagcc ttttggctct acctcctgcg gagcagcaga agaggcgcca
     5101 gcagcgcgaa aagcagcggc gacgcggagg cggcggagca tccgccgctc ccgccgcttc
     5161 tgctcatccc gggcgtcgag aagaccttga tgcagaagtt cgcctctgcg aagtcctccg
     5221 ttagatccgt ccacgaagcg ccgtccccgc tgtagaagct ctcgccgggg gaggccgtcg
     5281 cgttgtccga atactcctca agcggaagtt cgacgggcag ggggtactcg taccccggcg
     5341 tggtgagctt cacccgcacg cggaagcgcg ttccttctcc cactccgacc ggccggggca
     5401 gagggatcgt atggtatccc ggttgctcca gggtaccgga agcctccgcc gtacgaagcg
     5461 cttcccccga accggagacg ccgcccgata cgtcgtcgat gaacacctcg tacatcgttc
     5521 cgtacgccgc cgcgtagaac gaaaccgcgc ggagcgtctg atcctccccg gcggtgaaaa
     5581 tgttcgccat ccacccagtc tcgggattcg tctccgaggc gctcaccgag cgcacccacc
     5641 ccaacggatc gtgctggtgg atgatgccgt acaaatcagg ggaagcgaga atgtacgcga
     5701 tcccgctgtc gacgctcgta tcgtagtagg acatgtagaa gtacccgccc tcgccgaatt
     5761 cttctcccca gctgttccgg acaatccacg caccgtctcc cggaggcgtc gtcgcgaagt
     5821 tctccttcgg gaaaccgtcg tcccagccga ccacgttcac agcgtgattc gccccacggg
     5881 ggccgttgta gaaaaaagcg tgcgtttcag tattccacgt attctcgttg aagtccgcca
     5941 tcatgccgat ggagagcgcg ccgtactcca tgatg
//
LOCUS       DAOSWW010000036         5971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8780_length_5971_cov_3.887424, whole genome shotgun sequence.
ACCESSION   DAOSWW010000036 DAOSWW010000000
VERSION     DAOSWW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8780_length_5971_cov_3.887424"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK442_01440"
     CDS             complement(<1..294)
                     /locus_tag="PK442_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO86339.1"
                     /translation="MKQLRRLFLAAAVLLMVSGAFAAEKVVVYHAGSLAAPMAKIEAM
                     YEEANPGVDIQRESGGSAALARKITDLGGACDAFFSADYMVIEKLLRPATADWN"
     gene            complement(328..1356)
                     /locus_tag="PK442_01445"
     CDS             complement(328..1356)
                     /locus_tag="PK442_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO86340.1"
                     /translation="MIELENLSVNLRTFRLESLSLRIAPGEFFMIVGPSGAGKTILLE
                     SIAGLRSLEGGRVRIRGLDVTPLPPERRNVALVYQEYALFPHMNVERNIRWGLRFAAL
                     PDDAHVHRLIDLLKLKPLLGRSPETLSGGEQQRVALARALAVKPSVLLLDEPLSALDP
                     SFREDLRDYLAEINRGGMTLVMVSHDFGEVLSLGNRVAVISDGVLQQVGDVRSVFRAP
                     ANRSVAEFVGMKNLFPASIRGTDAVTEGGATLLLGKAAASGERLLGIRPEDVVIGAEH
                     PGDSRNAFQGKVVSIVPRGMAFEVIMRTDGGMRITARVLSSALLSGGVAVGGEYWASF
                     SPDSVHVL"
     gene            complement(1353..2147)
                     /locus_tag="PK442_01450"
     CDS             complement(1353..2147)
                     /locus_tag="PK442_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO86341.1"
                     /translation="MPRSLRERLFMLSMGSFLLLYILWPLGGLFLRADWSLVASASHD
                     GEVLSALWRSIWTAAAATGVIALFGTPLAYFLARQSFRGKSILEAVIDVPIMIPHTVA
                     GIAVLMAFSPKAPAGALLTSMGFSPVNSNLGIILACMFVSIPFYVDAARDAFYSVSPR
                     TEKVARTLGASFPHVFFRISLPLARKGLISGLIMSWARGVSEFGAIVIIAYHPMVAPT
                     LIYDRFATFGLKYSTPVAVQLILVCLGMFVVLRILAAGKRKEDAEI"
     regulatory      complement(2201..2320)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     regulatory      2463..2582
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            2660..3490
                     /locus_tag="PK442_01455"
     CDS             2660..3490
                     /locus_tag="PK442_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO86342.1"
                     /translation="MNLRKVCAFVLIAAFLFTGAGTGTAAEVLRMATTTSTDNTGLLD
                     YLAPLLLNEKGIEIRWVSVGTGKALEYGRNGDVDVVLVHDPEAEAKFVAEGSGVNRRQ
                     VMFNDFIIVGPASDPAKVQGLSVTAALKKIAQDGAPFASRADKSGTHMAELRLWAAAG
                     MPEPDKEKWYIQTGQGMLETLMIAGERKGYAFTDRGTFIKYEDSRKEQADLAIIVEGD
                     ASLKNQYSVMAVNPAKWENIRYDIALQYIDWLISPSTQKAIADFQLLGKQLFFPNAAR
                     "
     gene            complement(3571..3780)
                     /locus_tag="PK442_01460"
     CDS             complement(3571..3780)
                     /locus_tag="PK442_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOO86343.1"
                     /translation="MKISARNQIRGKVTAIKEGAVEAQVTLDIGGGQTMVSVITMDSV
                     ANLGLKVGSEAVAIVKADSVMLAVN"
     gene            complement(3866..4573)
                     /locus_tag="PK442_01465"
     CDS             complement(3866..4573)
                     /locus_tag="PK442_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86344.1"
                     /translation="MYPLNILIAEPDMMLRTLYRKTVSEVPGYVFAGYADNGDALHSI
                     VACTAVNVVLLDILLPRFGSLEGFRAMRAEFPRTDFIILSSEKTPDVVRGTICSGAFD
                     YLIKPFEWERFERALAAYSEYHRSLVDRKRPWRQEDIDRLPGFRKQLPENFQPTPKGF
                     QETLLNRLFNLLRESSSPLSASEAGRMLGISRSSARRYLEYLADEGEIAVQYEFTQVG
                     RPRKLYSVHFRAKEGSV"
     gene            4888..5640
                     /gene="modA"
                     /locus_tag="PK442_01470"
     CDS             4888..5640
                     /gene="modA"
                     /locus_tag="PK442_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO86345.1"
                     /translation="MSTHAIRVLLLVIFLFSSSLCSPLPGAASEEDVYVAVSPGIAQC
                     LEDMYASFAAAGGGKLVFVKEATGPLAQKMDAGAPFALLIAADPGWPKWLYERGKLKD
                     MRVCANGHLVLWAEKEEFCCEEKLASLRIASPDPETTSHGTLAADYLKGLGLWDEGAA
                     SGKIVITKNALHAIMAVQAGSADVALVPASLAIEMEGVYVQLPVPPLPTVGGLSTAHS
                     PESAAAFWKFLRSPEAAPLWEKWGFEAAEVAQ"
     gene            5637..>5971
                     /locus_tag="PK442_01475"
     CDS             5637..>5971
                     /locus_tag="PK442_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86346.1"
                     /translation="MSRPKTLDRIAAVVRERLERDSTGLLNEPLAIGSLDVGQAIGDP
                     APFTDFALQRGEERLVEARFLNARGQAFTSFPSAWKGTLGELLDLPLEEDCSRAFFIA
                     GVNAVGRYLG"
BASE COUNT         1228 a         1768 c         1840 g         1135 t
ORIGIN      
        1 gttccagtcc gccgtcgcgg ggcgcagtaa cttttcgatg accatgtagt ccgccgagaa
       61 gaacgcgtcg cacgctccgc cgagatcggt gatcttacgc gccagagcgg cgctgccgcc
      121 cgactcgcgc tggatgtcca cgccggggtt ggcctcttcg tacatcgcct cgatcttcgc
      181 catgggagcg gcaaggctcc ccgcatggta gacgacgacc ttctccgccg cgaacgcgcc
      241 ggagaccata agaagaaccg ctgccgcaag gaacaacctt cgtaactgtt tcatgaacaa
      301 cacctccgtt aaagtatgat gcaccatcta caaaacatgg actgaatccg gactgaaact
      361 cgcccagtat tcgccgccga cggcgactcc tcccgagaga agcgccgacg aaagaacccg
      421 tgccgtgatg cgcatgccgc cgtccgtgcg cataatcact tcgaacgcca tcccccttgg
      481 aacgatggag acgaccttcc cctgaaacgc gttccgggag tctccggggt gttcggcgcc
      541 gatgacgacg tcctcggggc ggatgccgag cagccgttcg ccggaagccg ccgccttgcc
      601 gaggaggagc gtcgcgcccc cctccgttac ggcgtccgtg ccccgaatgc tcgccgggaa
      661 gagattcttc atcccgacga actcggcgac gcttctgttc gccggcgccc ggaagaccga
      721 ccggacatcc cctacctgct gcaaaacgcc gtccgagatc acggcaaccc tgttcccgag
      781 cgaaagtacc tcgccgaaat cgtggctcac catcaccagc gtcataccgc cgcgattgat
      841 ctccgccagg tagtcccgca aatcctccct gaaggacgga tcgagcgcgg agagcggctc
      901 gtcgagcagc aggacggagg gcttcacggc gagcgcgcgg gcgagcgcga cgcgctgctg
      961 ctcccccccg gagagcgtct ccggagatcg cccgagcagc ggcttcagct tcagcaggtc
     1021 gataagacgg tgtacatgcg cgtcgtcggg gagggcggcg aagcggaggc cccagcggat
     1081 attccgctcc acgttcatgt gcggaaagag cgcgtactcc tggtagacga gcgcgacgtt
     1141 ccgccgctcg ggcggcaggg gcgtgacgtc gagcccccgt atcctgaccc ttcccccctc
     1201 gagggagcgc agcccggcga tcgattccag caggatcgtc ttccccgcgc cgctcggccc
     1261 cacgatcatg aagaactcgc ccggagctat ccggagggag agcgattcca gccggaaggt
     1321 acggagattg accgagagat tttcaagctc gatcatatct cggcatcctc cttccgcttc
     1381 cctgcggcca gtatccgcag aacgacgaac atgccgaggc agacgaggat gagctggacg
     1441 gccacaggcg tggagtactt cagtccgaag gtggcgaacc ggtcgtagat cagcgtgggg
     1501 gccaccatcg ggtggtaggc gatgatgacg atcgccccga attcgctcac tccccgcgcc
     1561 cagctcatga tgaggccgga gatcagcccc ttcctcgcca gggggaggga gatgcggaag
     1621 aaaacgtgag ggaatgaggc cccgagcgtc cgggccactt tctccgtccg cggggagacc
     1681 gagtagaagg cgtcgcgtgc cgcgtccaca tagaacggaa tggagacgaa catgcatgcg
     1741 aggatgattc cgagattgct gttcacagga gagaatccca tggaggtgag cagcgccccc
     1801 gccggagcct tcggcgagaa ggccatgagc accgcgatgc ctgctacggt gtgaggaatc
     1861 atgatgggca cgtcgatgac cgcttcgagg atcgactttc cgcgaaagga ctggcgcgcc
     1921 aggaagtagg caagaggcgt cccgaagagc gcgatgactc ccgtcgccgc cgcagccgtc
     1981 cagatgctgc gccacagtgc ggagagcacc tccccgtcgt gcgacgctga ggcgacgaga
     2041 ctccagtcgg cgcgcagaaa gagtccgccg agcggccaga gaatatacag cagcaggaag
     2101 gaacccatgg acagcatgaa caaacgctcc cgaagcgaac gaggcatatt ccaccctcca
     2161 gacacataaa aagggccgtc tccatgggag acagccctgg gatacaccgg ccgctcctcc
     2221 tttccgaacg cgggaggccc tcccgtttcc gggagaaccc gttggccgtc tcccatggaa
     2281 cgtattccgt agggagtgcg gctcggagga tgtccgaaaa acccggacga agcaagaata
     2341 ccacgtagtt cagaaaatga caatgatact ctttatcaag taaaggcgaa aaatgctcgt
     2401 tctcatgcct gtcggcggat acctcgactc gaggttttat ggtatgatat tcatcaaata
     2461 aaagacaact tgttctccga gcccgtttgc ctacaggaaa ttcccacggc ggcgggccgt
     2521 cagggttcgt ccggaaacag gcgagcctcc cgtgttcggc aaggagatgg attcggatgc
     2581 tgatgcagcc gtctcccgcc gtatatgggg gaggcggctt tttgtatgcg ggacaaaatc
     2641 atatcgagga ggaatgaaaa tgaatttgag gaaggtatgt gcttttgtat tgatcgcggc
     2701 atttcttttt accggagccg gtacgggaac ggcggcagag gtgctccgca tggccaccac
     2761 caccagcacc gacaacacgg gactgcttga ttacctcgcc cctctgcttc tgaacgaaaa
     2821 gggcatcgag atccgctggg tctccgtcgg cacggggaag gcgcttgagt acggccggaa
     2881 cggcgacgtc gacgtcgtgc tcgtccacga tcccgaggca gaggcgaagt tcgtcgccga
     2941 aggttcggga gtgaaccgcc gacaggtgat gttcaacgac ttcatcatcg tcggcccggc
     3001 ttccgatccg gcgaaggtgc agggactctc ggtgacggcg gcgctgaaga agatcgcgca
     3061 ggacggcgcg cccttcgcga gccgcgcgga caagtccggc acgcacatgg ccgagcttcg
     3121 tctttgggcc gccgccggaa tgcccgagcc cgacaaggag aagtggtaca tccagaccgg
     3181 ccagggaatg cttgagacgc tgatgatcgc gggcgagcgt aaggggtacg ccttcaccga
     3241 ccgcggcacc ttcatcaagt acgaggactc gcgcaaggag caggccgacc ttgcaatcat
     3301 cgtcgagggc gacgcctcct tgaagaacca gtacagcgtc atggcggtga accccgcgaa
     3361 gtgggagaat atccgctacg acatcgcgct gcagtacatc gactggctga tctccccgtc
     3421 gacgcagaag gcgatcgccg acttccagct cctcgggaag cagctcttct tccccaacgc
     3481 cgcccgctga acgacggcct cagtacgttt tacaaaataa aaaaagcccc cgacaggggg
     3541 cttttttggt gtctgattct tgcggaatta ctaattgacc gcgagcatga cgctgtcggc
     3601 cttgacgatg gcgacagctt cggatcccac cttgagaccg aggttggcca cggagtccat
     3661 cgtgatcacg gagaccatcg tctggccgcc gccgatgtca agcgttacct gcgcctccac
     3721 ggcgccttct ttgatggcgg tcaccttgcc gcggatctga ttgcgtgcgc ttattttcat
     3781 gatatgcact cccttctgtt ctagattctt ccggtattat cgcacctaaa tacatctttt
     3841 gcaagtttgt gcagttgaaa agagttcaga cagatccttc ttttgcccga aagtgaaccg
     3901 agtagagctt tctcgggcgg cctacctgtg tgaactcgta ctgtaccgcg atctccccct
     3961 catcggcgag gtactcaagg tagcgccggg cgctcgacct ggagatgccg agcattcgcc
     4021 ccgcttccga agcggagagg ggactggacg actcccgcaa aagattgaag agtcgattca
     4081 ggagggtttc ctgaaaacct ttgggcgtcg gttgaaaatt ttcgggaagc tgcttccgga
     4141 aaccgggaag cctgtcgata tcctcttgtc tccaggggcg ttttctgtcg acaaggctgc
     4201 ggtggtactc gctgtatgcg gcaagcgctc gttcaaatcg ctcccactcg aacggtttga
     4261 tcagatagtc gaacgcgccc gagcagatcg ttcccctgac gacgtcgggc gttttctccg
     4321 aggagaggat gatgaagtcc gtccttggaa attccgcgcg catcgcacgg aatccctcca
     4381 ggctgccgaa gcggggcaga agaatgtcca agagaaccac gttgacggcg gtgcaggcga
     4441 cgatggaatg caaagcgtcg ccgttgtccg cgtagccggc gaagacatag ccgggaactt
     4501 ccgagacggt ctttctgtac agcgtgcgaa gcatcatgtc cggctcggcg atgagaatgt
     4561 tcagcggata cacttccagc tctcccttct acggaatgtc ctggagattc cctctcccaa
     4621 ggcgcgctcc atttttttct gatagtgaac ggcaggcccg cggcgggagg gaaaaaacta
     4681 cattaccatt atacatgaag cggaccgctt cttcgttttt ccggttttcc gtgcggagag
     4741 aaagaggggg cggcgctgtg aactccgaag tgaaggatga tttctacaag aggaatcgag
     4801 attgtgcaac gatgttgcat ttctctgttt gaggtggtat tcttgcagaa aatgcaacga
     4861 agttgcaaaa tccaggaggt gccttcggtg tccacacatg caattcgcgt gcttttgctc
     4921 gtcatttttc ttttttcatc atcattatgc tccccgttgc ccggcgcagc gtccgaagag
     4981 gacgtctacg ttgcggtgtc gcccggcata gcgcagtgcc tcgaggacat gtacgcctcg
     5041 ttcgccgcag cgggcggggg aaagctcgtc ttcgtgaagg aagccaccgg gccgctggcg
     5101 cagaagatgg acgcgggcgc tccgttcgct cttttaatcg ccgccgatcc ggggtggccg
     5161 aagtggctct acgaaagagg caagttgaag gatatgcggg tctgcgcgaa cggccatctt
     5221 gttctctggg cggaaaaaga agagttttgc tgcgaagaaa agctcgcctc gctccgtatc
     5281 gcctctccgg accctgagac gacgtcccac ggcacgctcg ccgcggacta tctgaagggg
     5341 ctcggtctgt gggatgaggg agcggcgtcg ggaaagatcg ttatcacgaa gaacgcgctc
     5401 catgcgatca tggccgttca ggcggggagt gcggacgtcg cgctcgttcc ggccagcctc
     5461 gcgatcgaaa tggaaggggt gtatgtacaa cttcccgtcc ctccgcttcc gaccgtcggc
     5521 ggcctgagca cggcgcattc gccggagtct gccgcagctt tctggaaatt tctgcgctcc
     5581 cctgaagcgg ctcccctgtg ggagaagtgg ggctttgaag ccgcggaggt cgcgcaatga
     5641 gccggccgaa gacgctggac cgtattgcgg cggtcgtcag ggaacgtctt gagcgagatt
     5701 ccacgggact cctgaacgaa ccgctggcga taggctctct tgatgtcgga caggccatag
     5761 gagacccggc gccgtttacg gatttcgccc tccagcgggg agaggagcgt cttgtggagg
     5821 cgcgcttcct gaatgcgaga gggcaggcgt tcacttcgtt tccctcggcg tggaagggaa
     5881 cgctcgggga actgctcgac ctgcctcttg aagaggattg cagccgggcc tttttcatcg
     5941 ccggagtgaa cgccgtcggc aggtatctcg g
//
LOCUS       DAOSWW010000037         5914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8895_length_5914_cov_3.119816, whole genome shotgun sequence.
ACCESSION   DAOSWW010000037 DAOSWW010000000
VERSION     DAOSWW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8895_length_5914_cov_3.119816"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="PK442_01480"
     CDS             <1..1525
                     /locus_tag="PK442_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOO86347.1"
                     /translation="KEGGFYLFREEDAVFYQAFASSPAAKERIEHELQLLIDDGTIAW
                     VIQTERPVTVRLSSGEDAFLHALAAPGRTMGAFIGILREPKEEILDISYALLSIYFSQ
                     VAGVLQNRELLRMARIENRHLTSTVDVLRETEEKLVSLNRTLEEMVEERTRELEKTNR
                     LLKEEIAERKRKEEALLESEELSASLLNHSPHPILVVNPDTSVKYVNFALEQITGYSL
                     VEVINRKAPYPWWGEEKLSTVSEYYRMVQSGETAKKEYCFRKKNGSPLWVEVSMTSVS
                     GSAGAKYAVANWVDITDRKVAERARENAERLLTETNAKIQDTVGGIVISMAKVVETRD
                     PYTAGHQERVARLAVAIAAEMGLSEEQIQGVGVAAVLHDLGKIDIPAEILSKPSRLKE
                     KEYSLIQDHCVAGYEILKNVDFPWPVAQAVLQHHERMDGSGYPNSLSGDDILLEARIL
                     AVSDVVEAMSSHRPYRPSLGLEQGLAEVAMYRGSRFDERVVDACMAIFARGESPWTE"
     gene            1922..3592
                     /locus_tag="PK442_01485"
     CDS             1922..3592
                     /locus_tag="PK442_01485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO86348.1"
                     /translation="MKKNLLRCELDTAFLHGSVVTVNEKEEVAEALGIAGNRIAYVGN
                     DRGIEPFISENTRVVDLAGRTLMPGINDTHFHPILTGLLGAHHDSGMIDTTRVRCASL
                     AGMLGMLREAVGRKRPGEWISMMGYEPELFTDERRHPTIGELDAIAPENPVHCMHGGG
                     HICMYNTKALAYLDVHIPEDAGKYPPDEVEVVDGRLTGMVRGHTHFKLWGMVGYTESQ
                     QAAAAMKSHRRALEAGITSIGDMGECGPVSYHIMQKLCRDRTFKIRVNMALHSIFGKS
                     YSLNESEHWLQLGFMSGLGDERFRVGPCKFMIDGGSGGPSCATREPYSHDPDMVRERG
                     WEREETARYIERIVGAECQCTAHAIGDLAVEFMVEGYERCFRKDPEKFRRMRHRIEHC
                     SLTDQDLIDRMARMNICPSVNAGMIQAIGANYMKFYGERNAYMAALRSMFDAGVMCSL
                     SSDAPSGPIGIQTIDGAVNRYDRNQNVQCDKSQAVSVTEAIRAATYNGAYSSFEEHIK
                     GSLEVGKLADMIVLSDDILKIDPMDIHTLRVDCTVIDGLFEYERAEEA"
     gene            3597..4877
                     /locus_tag="PK442_01490"
     CDS             3597..4877
                     /locus_tag="PK442_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HOO86349.1"
                     /translation="MESAMIERIRASAEALRPRMEAFLSDFLKIDTENPPGRNYEKCV
                     RFLGEKMRSLGCEVEYVEVPEEALASLPEESKGFPRYIVVGTFKGTRERPAIHFSGHY
                     DVVPAGGGWTVDPYGGLIADGKIFGRGASDMKSGIAAQIFALETLQHAGIPLKGSFVS
                     SAVPDEETGGQPGMGYLVRSGRLTKENTDYCIITEPLDPDMICLGHRGTLWFTLTFKG
                     VQSHGSMPSEGLNPIEGMRALLERIDRRIRPQLERVSKYPVSPERSRKSTLCITTIQA
                     GSKVNTIPDTCSASFDWRLIPEQSVEWAKGEILSICDDLVREGVILEYAYTPIIEADP
                     TMVDEDQPLVDALKTCGKAVLGKEMGINFSPGMDDQRFAVHEGGLGAAVVYGPGRLAL
                     AHKSDEHIAVDDLVKGVEIMAAVAAELLGTKERQ"
     gene            5031..5849
                     /gene="panB"
                     /locus_tag="PK442_01495"
     CDS             5031..5849
                     /gene="panB"
                     /locus_tag="PK442_01495"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOO86350.1"
                     /translation="MSVLDFKKYKEEGRKFAYVTAYDYTTASIVNESEVEVILVGDSL
                     GMIMLGYKDTVAVTMEDMIHHTRPVVKGAPDTFVVGDMPFGSYNKSIEQGVENATRLV
                     KETGCDCVKLEGGVKYAPLVRAIVDAGISVMGHIGLTPQNATQLGGFKVQGGTPESAR
                     QLIEDAKELEKAGAFSIVLECVPKVVGKAVSEAVKIPILGIGAGPFVDCQVLVTQDLL
                     GMYGDFKPKFVKLYANVRKVMVDGLNQFHKEALDGEFPSDEYSFNKEVNLEKLY"
BASE COUNT         1264 a         1755 c         1928 g          967 t
ORIGIN      
        1 gaaggagggc ggtttctacc tcttccggga ggaggacgcg gtcttctatc aggcgtttgc
       61 cagctcgccc gccgcgaagg agcgtatcga gcatgagctt cagcttctga tcgacgacgg
      121 aaccatcgcg tgggtcatcc agacggagcg ccccgtgacg gtgcgtctct catccgggga
      181 ggacgccttt ctccacgccc tcgctgcgcc ggggaggacg atgggggcgt tcatcggcat
      241 cctgagggag ccgaaggagg agatcctcga catcagctac gccctgctct ccatctactt
      301 cagccaggtg gccggagtgc tccagaacag ggagctgctc cgcatggcga gaattgagaa
      361 ccgtcatctt acctcgacgg tggacgtgct gcgggagacg gaggagaagc tggtctccct
      421 caaccggacg cttgaggaga tggtcgagga gcgcaccagg gagctggaga agacgaacag
      481 actcctgaag gaggagatcg ccgagaggaa gcggaaggaa gaggccctcc tcgagagcga
      541 ggagctctcc gcgagccttc tgaaccactc gccgcacccc atcctcgtgg tgaaccccga
      601 cacctcggtc aaatacgtca acttcgccct cgaacagatc acgggctact cgctggtcga
      661 ggtgatcaac cggaaggccc cctacccgtg gtggggagag gagaagcttt cgaccgtttc
      721 cgaatactac cggatggtgc agtccgggga gaccgcgaag aaggagtact gcttccgcaa
      781 gaagaacggc tccccgctct gggtggaggt gagcatgacc tccgtatcgg gaagcgcagg
      841 cgccaagtac gcggtcgcca actgggtgga catcaccgac aggaaggtgg cggagcgcgc
      901 cagggagaac gccgagcgtc tgctcaccga gacgaacgcg aagatccagg acacggtcgg
      961 cggcatcgtg atctccatgg ccaaggtggt cgagacgcgg gatccgtaca ccgcgggcca
     1021 tcaggagcgg gttgcccgcc ttgccgtcgc catcgcggcc gagatggggc tctccgagga
     1081 gcagatacag ggagtgggcg tcgccgccgt gctccacgat ctcgggaaga tcgacatccc
     1141 ggcggagatt ctgagcaagc cctcccgcct gaaggagaaa gagtacagcc ttattcagga
     1201 ccattgcgtc gccggatatg aaatcctcaa gaacgtcgac ttcccgtggc ccgtggccca
     1261 ggccgtcctc cagcaccacg agcggatgga cggatccgga tacccgaaca gcctctcggg
     1321 agacgacatt ctgctggagg cgcgtatcct cgccgtctcc gacgtggtgg aggcaatgtc
     1381 ctcccaccgc ccctaccggc cgagcctcgg cctcgaacag gggctcgccg aagtcgctat
     1441 gtacagggga tcgcgcttcg acgagagggt cgtggacgca tgcatggcca tcttcgccag
     1501 aggagagtcc ccctggacgg agtagaacgc gccgcgaaga aagcggagat gctgcaaacg
     1561 ctgataatga gcgccctttt tccctcccgg gaagaggggc gcttgtgtat tgcgaggagg
     1621 cggggaatga cgcccggaac ctgcttcgtt cccggagctg gaacgctgcg gaagttccgc
     1681 gaagcctccg catacggaaa gaaacaggcg cctcggtgag taagttgtta aaaaattatc
     1741 aatgttttgc gtcgcagaga aagtattgac aaaacaggcg gtacggctac aatgctttca
     1801 atagtccata tagagcaacg ctattccata atgcggaatt attccgagtc tctctcggcg
     1861 aaacggctga aatcctcccg ggagagtctc cggattcggt ccgaaatgag gagtgggtcc
     1921 gatgaagaaa aatttactgc gctgcgaact ggatacggcg tttctccacg gaagcgtcgt
     1981 caccgtcaat gagaaagaag aggttgccga ggcgctcggg atcgcgggga accgtatcgc
     2041 ctacgtcggg aacgaccggg ggatcgagcc gttcatctcc gaaaacaccc gcgtcgtcga
     2101 tcttgccgga cggacgctca tgcccggaat caacgacacg cactttcatc cgatcctcac
     2161 cggccttctc ggcgcacacc acgactcggg gatgatcgac acgacgcgcg tgcgctgcgc
     2221 ttccctggcc gggatgctcg gcatgctccg ggaggccgtc ggacgcaagc ggcccggaga
     2281 atggatctcc atgatggggt acgagcccga gctctttacc gacgagcgac ggcaccccac
     2341 tatcggcgag ctcgacgcca tagcgcccga aaatccggtg cactgcatgc acggcggcgg
     2401 gcatatctgc atgtacaaca ccaaggcgct cgcctacctc gacgtgcaca tccccgagga
     2461 tgcgggaaag taccctccgg acgaagtcga ggtcgtcgac ggaaggctca ccggaatggt
     2521 gcgcggacac acgcacttca agttgtgggg gatggtcggc tacaccgaat cgcagcaggc
     2581 cgcggcggcg atgaaatcgc accggcgggc tctggaagcg ggcattacct ccatcggcga
     2641 catgggcgaa tgcggcccgg tctcgtacca catcatgcag aagctctgcc gcgaccggac
     2701 cttcaaaatc cgcgtcaaca tggcgctcca cagcatcttc ggcaagagct actccctgaa
     2761 tgagagcgaa cactggctcc aactcggctt catgtccgga ctcggcgacg agcggttccg
     2821 cgtggggccg tgcaagttca tgatcgacgg agggtcgggc ggcccctcct gcgcgacccg
     2881 cgagccctac tcccacgacc cggacatggt gcgcgagcgc ggctgggagc gcgaggagac
     2941 ggcgcgctat atcgagcgga tcgtcggcgc ggagtgccag tgcaccgcgc acgccatcgg
     3001 cgatctggcc gtcgagttca tggtcgaggg gtacgagcgc tgtttcagga aagaccccga
     3061 aaaattccgc aggatgcgcc accgcatcga gcactgttcg ctgaccgatc aggacctgat
     3121 cgaccgcatg gcgcgcatga acatctgccc ctccgtcaac gcgggcatga tccaggcgat
     3181 cggggccaac tacatgaagt tctacgggga gcgcaacgcg tacatggcgg ctctccgcag
     3241 catgttcgac gcgggcgtca tgtgctcgct gtcgagcgac gcgccctccg gtcccatcgg
     3301 cattcagacc atcgacgggg cggtgaaccg ctacgaccgc aaccagaacg tccagtgcga
     3361 caaaagccag gcggtctccg taacggaggc gatccgcgcc gcgacgtaca acggcgccta
     3421 ctcctcgttc gaggagcaca tcaaggggag tctggaagtc ggcaaactcg ccgacatgat
     3481 tgtcctctcg gacgatattc tgaagatcga ccctatggat attcatacgc ttcgggtgga
     3541 ctgcaccgtc atcgacgggc tgttcgaata cgaacgagcg gaggaggcgt aacggtatgg
     3601 aaagcgccat gatcgaaaga atccgcgcgt cggcggaggc gttgcggccg cgcatggagg
     3661 cgtttctcag cgactttttg aagatcgaca cggaaaaccc gccggggcgc aactacgaga
     3721 agtgcgtcag gtttcttggc gagaagatgc ggtcgctcgg atgcgaagtg gagtacgtcg
     3781 aagtgccgga ggaggcgctc gcgtcgctcc cggaggagtc gaaagggttc ccgagataca
     3841 tcgtcgtcgg cacgtttaaa gggacgaggg agcgtccggc gatccacttt tcggggcact
     3901 acgacgtcgt cccggccgga ggggggtgga cggtcgaccc ctacggaggc ctgatcgccg
     3961 acgggaaaat tttcgggcgc ggcgcgagcg acatgaagtc cggcatcgcc gcgcagatct
     4021 tcgccctcga aacgctccag cacgcgggca ttccattgaa ggggagcttc gtctccagcg
     4081 cggtccccga cgaggagacc ggaggacaac ccggcatggg gtacctcgtc cggagcggaa
     4141 ggctgacgaa ggagaacacc gactactgca tcatcaccga gccgctcgac cccgacatga
     4201 tctgcctcgg gcaccgcgga accctctggt tcacccttac cttcaaggga gtgcagagcc
     4261 acgggagcat gccgtcggag gggctgaacc ccatcgaggg tatgagggcg ctgctcgaac
     4321 gcatcgaccg gcgcatccgt ccgcagctgg agcgcgtcag caaatacccc gtcagccccg
     4381 aacgctcccg gaagagcacc ctctgtatca ccaccatcca ggccggatcg aaggtcaata
     4441 cgattcccga cacctgctcc gcgagcttcg actggcgcct catccccgag cagagcgtgg
     4501 agtgggcgaa gggggagatt ctctccatct gcgacgatct tgtgcgcgag ggcgtcatcc
     4561 tcgagtacgc ctatactccg atcatcgaag ccgaccccac catggtcgac gaagaccagc
     4621 cgctggtcga cgcgctgaag acctgcggga aggccgtcct ggggaaggaa atggggatca
     4681 acttcagccc cggaatggac gatcagcgct tcgccgtcca cgaaggcggc ctcggggcgg
     4741 ctgtggtgta cgggcccggg cgtcttgcgc tcgctcataa atcagacgag catatcgccg
     4801 tcgacgacct cgtcaagggc gtggaaatca tggcggcggt cgcggcggag ctgctcggga
     4861 cgaaggagcg acagtagccg gatagtgatc acggcaaagt ttttggcgtt cattcgccgc
     4921 cggcgcgtct tctgacgtcc gccgcgcggc ggaatgaaac gatacagagc ggcacgcata
     4981 cgcggccgct atctcggcag gaaagaggag ggcaattcat gaagaagaag atgtcggtgc
     5041 ttgatttcaa gaagtacaag gaagagggca gaaaattcgc ctacgtgacg gcctacgact
     5101 acaccacggc gtccatcgtc aacgaaagcg aggtcgaggt catcctcgtc ggcgactccc
     5161 tcggcatgat catgctgggg tacaaggaca ctgtggcggt aaccatggag gacatgattc
     5221 accataccag acccgtcgtc aaaggcgcgc ccgacacgtt cgtcgtgggc gacatgccgt
     5281 tcggctcgta caacaagagc atcgaacagg gcgtggaaaa cgcgacgagg ttggtcaagg
     5341 agacggggtg cgactgcgtg aagctggaag gcggcgtcaa gtacgcgcct cttgtgcggg
     5401 ccatcgtgga cgcgggaatt tcggtcatgg ggcacatcgg cctcacgccg cagaacgcca
     5461 cgcagctcgg ggggttcaag gtgcagggcg gaacgccgga gagcgcgcgg cagctcatcg
     5521 aggacgcgaa ggagcttgag aaggcgggcg ccttttccat cgtgctggag tgcgtcccga
     5581 aggttgtcgg caaggccgtc tccgaagcgg tgaagattcc catcctcggc atcggcgcag
     5641 gccccttcgt ggactgccag gtgcttgtca cacaggatct gctgggcatg tacggcgact
     5701 tcaagccgaa attcgtcaag ctgtacgcca acgtccgcaa ggtcatggtg gacgggctga
     5761 accagttcca caaagaggcg ctcgacggag agttcccgag cgacgagtac agcttcaaca
     5821 aagaggtgaa tctggagaag ttgtactagg catctgccgg cagaagcagg aaggaggtcg
     5881 cggaagacca tgacggaaag aacggaatcg cagg
//
LOCUS       DAOSWW010000038         5911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8900_length_5911_cov_4.253757, whole genome shotgun sequence.
ACCESSION   DAOSWW010000038 DAOSWW010000000
VERSION     DAOSWW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8900_length_5911_cov_4.253757"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PK442_01500"
     CDS             <1..671
                     /locus_tag="PK442_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86351.1"
                     /translation="ERILEASRSYNLSGQYMKAAEKASEALDFEQSPEALSNLGYAHI
                     GLGNYPAAFTAYWRRLLLSPKLRKVSLEPPAFLSLPFKEGRWKEIPVPNPRDLPVVVR
                     SEKMVLLPGGNAVLSGVLPGKEYLFEALRINPTGKDVYLNISLSYLMMQNKGTSSPSF
                     SAAPARSENENALYLALLAHALREKKADEQSEQFLNAAAARASGEVLRLVRSLEKKTS
                     PETK"
     gene            914..1996
                     /locus_tag="PK442_01505"
     CDS             914..1996
                     /locus_tag="PK442_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HOO86352.1"
                     /translation="MNATKVDELGKKYMRQYSLYLEFAARACNLLENLVEQDGIQLYN
                     VSGEAKTPEGFLRMYEALAEGVPSSLSEIADLCTVKILVHFPEDVEKVERIVQQEFLV
                     DMARSVTSASLDDPDRFGYPAVYYTLSLSRARAAMREWSKYKDIPFSLEIRTVLQEVW
                     AANTPKVMLPTDTSTKKKIQRKLFRVGALLEEADEGYFSLWESARESAMPVTPPVARV
                     EPVASTRRVFDRNALRQYFQEHEEIPRRWAEIASDVGFPPFRPDPEYLETSLSYLWDI
                     LRASEMDSPDEFQRFIASLEEEEKGREQVATVYKAFEREAQSWRVDGYSALFLLVLNL
                     KWDVLQNKDLVELNIKRGSDRIKGIL"
     gene            2423..3880
                     /locus_tag="PK442_01510"
     CDS             2423..3880
                     /locus_tag="PK442_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOO86353.1"
                     /translation="MQPGVPGNTALLADGGDVLVRCLSAFQQRRAPLRYAFSFGEAVD
                     YSPLEAASSILQSDSQTDFYVFILKELREGRKFLDFASDAAKKGKRTAVLLLRRPQTS
                     SREETAALKQFGVVLLNDIQEIVDGALAFLALKNSDLSGGKECAYHTSSDELSMLIKK
                     EAERAEFTLQESARATSPLLIDVLSGSDSLNTVQRRKQRIEQSGRLPVFSSLADNALF
                     EEQLYSAKIPLIPGIRRCFNALQLVFGSGPAVCPAPSEGAHPTKCPLHPRPTEYDAKV
                     LLRAYDIPVAREALCHSLPEALTAAETIGYPIALKVMSPSILRKTEARVIALDVQNEE
                     ELKNAYGRTLEKARIANPSARIRGVLVQEMVHGGTEWRMEYRKDPRFGPVVEMSISGI
                     FGEIFPDGVLRAAPFDTDEALRMIKESKGYPLLLAGWRRERLDVEAFASSLAAFSRLA
                     YCETEIARLDVNPVFVNRIGVLVVDAFIERRGGIR"
     gene            3877..4488
                     /gene="plsY"
                     /locus_tag="PK442_01515"
     CDS             3877..4488
                     /gene="plsY"
                     /locus_tag="PK442_01515"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOO86354.1"
                     /translation="MTALIWGTLGYLAGSFPTGYLAGLWVKGVDIRTIGSGGTGATNA
                     GRLLGKNWAKAVAIVDMLKGAVPMLCARWWGISDPWIIALIAFAGVVGHNYPVWLSFK
                     GGKGVATSYGVAFFLYPHLSFFVAPAGGLVWLLVLKAKGYVSLASMTSLCCLPFFALV
                     LSFPLPYVLVLGALALLAIFRHKDNIKRLREGKESRFGKHAKS"
     gene            4917..5396
                     /locus_tag="PK442_01520"
     CDS             4917..5396
                     /locus_tag="PK442_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOO86355.1"
                     /translation="MASLTRVIENYINNLFSEMDEQTVSLRRKELAETFGCVPSQINY
                     VLRSRFTPERGFIVESQRGGHGYIRIVRICYDIPEEKVNHIDEIVGDSITEQEARRIL
                     TSLQERGMIDSRERLLIEVALRHTDELGSTEFDISPYKRGALAAELLKRLLRSLALA"
     gene            5445..>5911
                     /locus_tag="PK442_01525"
     CDS             5445..>5911
                     /locus_tag="PK442_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOO86356.1"
                     /translation="MWHFFTERGRKVVQLAHREALRLGHDVVGTEHVLLAILSEGEGA
                     GVQTLEALGVNLAELRSRIESTVGKSHPILKPVDLPLSPRAKRVFDLSVREARNMGVN
                     YVGTEHILLGILAEGESAAARVLLGLGIDHAILAKEIMRFLEGENGSGKGTETS"
BASE COUNT         1362 a         1384 c         1600 g         1565 t
ORIGIN      
        1 aagagagaat tcttgaggca agccgttcgt acaatctctc cggacagtat atgaaagcgg
       61 cggaaaaggc gtccgaagct cttgacttcg agcaatctcc ggaggctctc tcgaatctgg
      121 gatacgctca tatcggtctg ggcaattacc cggcggcttt taccgcctac tggagacggt
      181 tgctgctttc tccaaagttg cggaaggttt ctcttgaacc tcccgcattc ctctcccttc
      241 cgttcaaaga agggcgctgg aaagagattc ctgttccgaa tccaagagat ctgcctgttg
      301 ttgttcgcag cgaaaaaatg gtgcttctgc cgggtgggaa cgcagtcctt tccggagttc
      361 ttccaggcaa ggagtatctt ttcgaagcgc ttcggataaa tccaacaggg aaagacgtat
      421 atcttaatat ttcattgagc tatcttatga tgcagaacaa gggaacttct tctccgtcat
      481 tttctgccgc tccggccaga agtgagaatg aaaacgcgct ctatcttgcc ttgcttgccc
      541 acgcgctccg tgaaaaaaaa gcggatgagc agtcggaaca gtttcttaat gctgcagcgg
      601 caagagcatc gggagaggta ttgcgcttgg ttcgttccct ggagaagaaa acttctcctg
      661 aaacaaagta aaatgcagat gtacgcttga acgcttttct ttcaccagac gcggttcaaa
      721 tcgcgcttga attcgaggat tgagtaagag cttttcaata aagctgattt actatatact
      781 tgcctgtgga ctgtctgttc cgtactcgcc ttaaaaaaag ggtttgctgc aagctgccct
      841 gggctggttt ctgtggtgga tcgagaagcg tgcaaactcg aagcacttta tttttatggg
      901 cggtgattaa tcgatgaatg caacgaaagt tgacgaattg ggaaagaagt acatgcgaca
      961 gtactctctt tacctcgagt ttgccgcgag ggcctgcaat ctattggaga accttgtgga
     1021 gcaggatggc attcaactgt ataacgtgtc tggagaggca aaaaccccgg agggtttcct
     1081 tcggatgtat gaggccctcg ccgagggagt tccctcatct ctttcagaaa ttgccgatct
     1141 ctgcaccgtc aagattcttg tccatttccc agaggatgta gaaaaagtgg agcgaattgt
     1201 acaacaagaa ttccttgtgg atatggcacg gtccgttaca tcggcaagct tggatgatcc
     1261 cgatcgtttc ggttatcctg ctgtgtatta tacgctctcc ctaagccgcg cacgggcagc
     1321 aatgcgagag tggagcaagt ataaggacat tccgttttct ttggagatac gaacggttct
     1381 tcaggaggtc tgggctgcga atacaccgaa ggtcatgctt ccgacggaca cgagcacaaa
     1441 aaagaaaatt caaagaaaac tctttcgggt aggcgctctt ctggaagaag ctgacgaagg
     1501 gtatttttct ctttgggaat ctgcccgaga atctgcaatg cccgtcactc ctcccgttgc
     1561 ccgggttgag cctgtcgcat caacccgacg ggttttcgac agaaacgcat tgagacagta
     1621 tttccaagaa cacgaagaga ttccccgccg atgggcggag atagcttctg atgtcggatt
     1681 ccctccgttc aggcccgatc ccgaatatct ggagaccagt ctctcttatc tttgggatat
     1741 tcttcgtgct tccgaaatgg attctcccga tgaatttcag cggttcattg cctccctcga
     1801 agaggaagaa aaggggagag agcaagttgc tacagtgtat aaagcattcg aaagagaagc
     1861 gcagtcgtgg agggttgacg gctactctgc ccttttcctg ttggtgctga atctaaagtg
     1921 ggatgttttg cagaacaagg atcttgtgga gctcaatatc aagagaggtt ccgacaggat
     1981 caaaggaatt ctctagggag ttagatattt ttcccgcttt tcggatgggg agtagcggtt
     2041 cgctctcccc attgtttttg gcttgccaga aaggaagagc gttatggata caatagctcg
     2101 actgcccggg agaggtcaaa atgctttttt ttgcggagga aatcaaacga gttttcgagt
     2161 actgcaatta gctccgcagc tcacttttcc cgaactctcg ttccccgttt cttcgtcttc
     2221 tcccattccg gatacactgt ttttcgcagg aaaaggtcct gaagaccgga agatgctcga
     2281 aagatgtttt gaacttccca ttcccttcct tgtgcttggc ggccgttggc ctcgaaagct
     2341 ctctctctct ctttgctcca gagcaagtga aaatggaatg cgtattcttg gcccccgaag
     2401 tgaaggaatt atcgtaccct gtatgcagcc gggcgtccct ggaaacaccg cattgcttgc
     2461 tgatgggggt gacgttctcg ttcgctgcct ctccgctttt caacaaagaa gagcgcctct
     2521 caggtacgca ttctccttcg gcgaggcagt ggactattct ccgcttgaag ctgcctcctc
     2581 gattcttcaa tccgattctc aaaccgattt ctatgtgttt attctgaaag aattacgtga
     2641 agggcgtaag tttcttgatt ttgcatcgga tgccgcaaag aaaggaaaaa gaactgctgt
     2701 tcttcttctt cgccggcccc aaacgagttc ccgtgaggaa actgctgccc tgaagcaatt
     2761 cggcgtggtt ctcctgaacg atattcagga gattgtcgat ggggccctag cctttctggc
     2821 tttgaagaac agtgaccttt ccggggggaa ggaatgtgca taccatacct cctcggacga
     2881 actttccatg ttgataaaaa aagaagcgga aagagctgaa ttcactcttc aagagagcgc
     2941 tcgtgcgacg tcgccccttc tgatcgatgt cctttctgga tccgattctc tgaataccgt
     3001 tcagcgaaga aagcaacgta tcgaacagtc aggccggctt cctgtgtttt catctctggc
     3061 ggacaacgca ttgttcgaag aacagttgta ttcggcgaaa atcccgctta tccccggtat
     3121 tcgccgttgc tttaacgctt tgcagctggt cttcgggagc ggcccggcgg tgtgcccggc
     3181 tccgtccgaa ggcgctcatc ctacaaagtg tccactgcat ccccgtccga ctgaatatga
     3241 cgcaaaagtg cttcttcgag cgtacgatat acctgttgcg agagaagctt tgtgccattc
     3301 gctacccgag gctttgaccg cggcggagac aataggctac cctattgctc tcaaggtgat
     3361 gtctccatct attttgcgta aaacggaggc tcgcgttatc gccctggatg ttcagaacga
     3421 ggaagagctc aagaacgcct acggacgaac gcttgaaaaa gcgcgcattg ccaatccgtc
     3481 tgccaggatt cggggagttc ttgttcagga aatggtgcac gggggaacgg aatggcgtat
     3541 ggaataccgg aaggatccgc gttttggacc agttgtcgag atgagtatca gcggtatttt
     3601 cggagaaata ttcccggacg gagtcctgcg ggcagctccc ttcgacacag atgaggctct
     3661 caggatgata aaggaatcaa agggctatcc tcttctgctc gcagggtgga gaagggagcg
     3721 tcttgacgtt gaggcgttcg cttcttctct tgccgccttt tctcgccttg catattgcga
     3781 aacggagata gcccgccttg atgtcaatcc cgttttcgta aatcggattg gtgttttagt
     3841 cgtagacgct tttattgaaa ggagaggtgg aattcgatga ctgctttgat atgggggacg
     3901 ctcgggtatc ttgcggggtc tttccctacc ggctacctgg cgggtctgtg ggtaaaggga
     3961 gtggatattc ggacgatagg atcagggggg acgggggcga cgaatgccgg aaggcttctc
     4021 ggcaaaaact gggcgaaggc cgttgcgata gtggacatgt tgaaaggcgc ggttccaatg
     4081 ctttgcgcaa ggtggtgggg tatttcggac ccgtggatta tcgcgctcat tgcttttgcc
     4141 ggcgttgtgg ggcacaacta ccctgtttgg ctctctttca aaggaggaaa gggagtcgcg
     4201 acatcttatg gcgtcgcgtt cttcttgtat cctcatcttt ctttctttgt tgccccagcg
     4261 ggcggcctgg tatggctgct cgttctgaaa gcaaaaggtt acgtctcttt ggcttcaatg
     4321 acctctctct gttgcctgcc tttttttgcg ctcgttcttt ctttccctct tccgtatgtc
     4381 ttggtgttgg gcgcgcttgc cttgctggca attttccgcc acaaagataa tatcaagcga
     4441 ttacgagaag gaaaggaaag ccgcttcgga aaacacgcaa aatcctgaag aactgaaaga
     4501 actcgaatcc gcagacggta ggtccgtctc cggagtactc tgcggcagat tgcgcagagt
     4561 atcgtcaatg ctatctgctc aagcgggcaa gaagttgttt tctgttcgcc ggttgtgctc
     4621 ttctgtcttt aaaacctgtg gtagaggcag acacccctcc ttattgacta atactgacca
     4681 acatcgtaga atagcctata agagatgcgt tcaatctgag tacttgtttt cagattgaac
     4741 catatctttg ggaaaaatgc gtttattgtt tcactctttc tgcttcaagt ggagaaacga
     4801 ggatctgttt tccgccgagc ggtaaggtat gcgtgggata gattgtccgg agcagattgg
     4861 ttatacagtt cagtaacgtg ctgtttgtat ggaatttgca gtgtggaggg aataaaatgg
     4921 caagcctgac acgggtcatc gaaaactaca taaataacct tttctccgaa atggatgaac
     4981 agacagtttc acttcggaga aaggagcttg cggagacttt cgggtgtgtc ccgagccaga
     5041 tcaactatgt cctccgaagc cgcttcactc ccgagcgcgg ttttattgtg gagagtcaga
     5101 gaggcggtca cgggtatatt cgaatagtcc gtatatgcta cgatataccg gaagagaaag
     5161 taaatcatat tgatgaaata gtaggagatt ccatcaccga gcaggaagcc cggcgtatcc
     5221 ttacgtctct tcaggagcgc gggatgattg attcgagaga acgtcttctc attgaggtgg
     5281 cgttgcgaca cactgacgaa ctgggtagca ccgagtttga tatctctccc tataagagag
     5341 gcgccctggc ggcggaactg ttgaagcgtt tgcttcgcag tctcgctctg gcctgatggc
     5401 aggatgagaa cggcccttcg gggccatgta aagggtggtt tggaatgtgg cattttttca
     5461 cggaacgcgg caggaaagtg gtgcaactag cgcacagaga ggctctccgt cttggacatg
     5521 acgttgtggg aacagaacac gtcttgcttg ctattctctc cgaaggggaa ggggcgggtg
     5581 ttcaaaccct tgaagctctt ggagtgaatc ttgccgagct gcgctctcgt attgaaagta
     5641 ctgtaggcaa atctcatccg attttaaaac ctgtggattt gcctttgagc ccgcgggcaa
     5701 agagggtttt tgatctttcc gttcgcgaag cgcggaatat gggcgtgaac tatgtcggca
     5761 cggagcatat acttctcggt atcctcgctg aaggagagag cgccgccgcc cgtgtccttc
     5821 tggggctcgg catagatcac gctattttgg caaaagagat catgcgtttt ctcgaagggg
     5881 aaaacggttc cggcaagggg acggagacct c
//
LOCUS       DAOSWW010000039         5892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8935_length_5892_cov_4.793387, whole genome shotgun sequence.
ACCESSION   DAOSWW010000039 DAOSWW010000000
VERSION     DAOSWW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8935_length_5892_cov_4.793387"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PK442_01530"
     CDS             complement(<1..624)
                     /locus_tag="PK442_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO86357.1"
                     /translation="MISYFDMSELKDGVTRQVYLALRRDILNWRLLPGRRLSEKDIAG
                     RMNISKTPVREAFIKLQEEGLLEIRPQRGTFVSLMDMEQIEEVRFTRLCVESTVLSLF
                     IESPDENALASMRSILDLQEGAVPDRDIDAFVEYDDAFHREIFLACGKARTWSFIDHF
                     SAQYKRLRYLSLQNYLDFGIVLAEHRAILSACEKRAGEDARRCLSKHL"
     gene            1001..1663
                     /locus_tag="PK442_01535"
     CDS             1001..1663
                     /locus_tag="PK442_01535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO86358.1"
                     /translation="MNKRIQMRAAFLLLLVFFAIPSFAEASGYLRNGNGHTVALTFDD
                     GPRPGYVEPILAILREKDVRATFFLVGRYVLEHPELVRAIDAEGHTVANHTFYHNNLT
                     SLPPENVYREWRLCNEAVERVTGKKPRFARPPGGRYNTFVAERANEEGLRIVLWTNNP
                     GDYAASLDGPSLARKVLSRAKEGDIVLLHVGVAPTIDALPEIIDGYRRKGFSFVTVDE
                     MR"
     gene            complement(1670..3343)
                     /locus_tag="PK442_01540"
     CDS             complement(1670..3343)
                     /locus_tag="PK442_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOO86359.1"
                     /translation="MKDLFERTEFLLNGGVLTLLSDSLAAHAARGVILRPEGEDLTVL
                     EAFGAGVESLFLKGRARDIQNAFPSPFLREAYDVAAAPLPGEWPWGIRLRGRDWVFFI
                     LLSEEPPKELIDHMPSFAGLLSLWQKHQKTAGTEERLSKLAYMVLATKSTLASVFEPM
                     GLDYFGAFLCDVLTESLFPSRLSVVLDKGGRLSHLDGEDIQMPPRKGIFARDILSPVP
                     VRLEESMRDSIGEAVFHDLAGEWSAILPIIGGDVRLFCLLRWETLPGEEALNFMELLG
                     NVASKALSMSAMREEKERNMQELSRRAFLLNALHEAALKFLEQTSKEELLLQILDIFS
                     EMTQAPRAVLVAWQPHAGGYVVFASKEDGILQRTGRPAGFPIGILEGDRPSSFTMEEG
                     KRLFAFMEVPFLASLDAMEGMKTIFPLWDGDRLIAFIAVSEPLPGTVTMDLETLEILA
                     RTASAAMLSREWESARLASGKYLNVAALVHWESERIREEIVAEGGTPMLVKGPFGVSV
                     SAEQERLCRLVVRMEDATVCVAEEPSEALASFFPSAAGWTIQKFPAGQK"
     gene            3534..3950
                     /locus_tag="PK442_01545"
     CDS             3534..3950
                     /locus_tag="PK442_01545"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO86360.1"
                     /translation="MHSSDTTKKQPKKIAAPGQESRRFVRVECVLRAEFRLHNHDAWL
                     PAEVLDLSVAGMKLRFNPFQRGRTLLPEVFEWADSRFRFPLKREFFHLDGHFLKIYQR
                     SAGLFTAGVEFDEPSPEDQFKLVELYAEFRRRRGAI"
     gene            4045..>5892
                     /locus_tag="PK442_01550"
     CDS             4045..>5892
                     /locus_tag="PK442_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO86361.1"
                     /translation="MKLNRLGSRILVVVLALLIVSVGGIGFYAMKIAGDNTRRMVNDY
                     EISVANGLAHELDAVFNRFDGILQALSSLVSTRFTPAMLNADVADVVVRQFASQNGKF
                     VADLGAQSVEARSLFMIFNPEIFGVKKVFSVGFQRKDQDSLYMFMDSEQFSAAKLIDR
                     KEPSTSWFWTPLDTGRPYWGDLTRSETGEDLVTYTMPIIIGDKIAAVAGMSFDFSFVR
                     TMLASVKIYDTGYPFLMNRDLRFLYHPSLSFDGPDLRKVSDGSMASFADAMLKEPSGR
                     FSYFYEGDEKTLAYKSLANGYIVAAAATTAESLAAVGAMQRAVYIGIAAVLLVAVVVV
                     IVFSRSLAKPLQAVAAGARNVAETGDLTKTLSVKTSIEEIKDVASAVNDMIGGTAEAV
                     RNIIDNSRKVLARAEDMSAASEQSTASVQEVITLVGKVARNTQDSASAIEEANAGIEE
                     VSSGAQAGAQAAVETGERAQEISHAAEKGGKALDDMAGLIEKVSKSGEQVSAAVGDLA
                     KSVSGITGFVNTITQIADQTNLLALNAAIEAARAGEAGRGFAVVAEEVRKLAEESNRA
                     AGEVGRVIGEISGKTENAMKDQKGSAEQIKQLVARAKETKTVIDDVVVKV"
BASE COUNT         1191 a         1761 c         1857 g         1083 t
ORIGIN      
        1 cagatgcttc gagaggcagc gccgcgcgtc ctcccccgcc cgcttttcgc aggccgagag
       61 aatcgcccgg tgctccgcca ggacgatgcc gaagtccagg tagttctgaa ggctgaggta
      121 tcgcaaccgt ttgtactgcg cgctgaagtg gtcgatgaag ctccacgtgc gggctttgcc
      181 gcacgcgagg aagatctcgc ggtggaacgc gtcgtcgtac tccacgaacg cgtcgatgtc
      241 ccggtccgga acggcgccct cctgaaggtc gaggatggag cgcatggagg caagagcgtt
      301 ttcgtcgggg gattcgatga agagcgagag cacggtgctc tcgacgcaga ggcgggtgaa
      361 gcggacttcc tcgatctgct ccatgtccat gagcgagacg aaggtgccgc gctgggggcg
      421 gatttcgagg agtccctcct cctgcagctt gatgaacgcc tcgcggacgg gggtcttgct
      481 gatgttcatc cgcccggcga tgtccttctc gctgagccgc cttccgggaa gaaggcgcca
      541 gttcagtatg tccctgcgga gcgcaaggta gacttgccgc gtgacgccgt ccttcagttc
      601 gctcatgtcg aaatacgaga tcacggcaag gccctctttc cctgaagatc gccccggcgg
      661 ggagccgaag cgtgtcgcat aattcacgct agagaaaatg gaaaactgat atgttagtat
      721 gttagccatc gttttagtct ttgtcaaggg gagcgaacga aattttcttc actttttatt
      781 cagaggtgaa tcgagtttca gcgcacccgg agtttgaagt ttttcattga ctttgcatgt
      841 ggtttcgggg cgaggtttgt gaaaacagtt tcgcgtcgtt tgatacggac gcgggtgaag
      901 cctctttctg cctgcggatt cggcaaaatt gtgtggcgcg acgatgcgga gagtgctatc
      961 atgtgctctt tgacgcattc agaagaatgg aaaggtgact atgaacaaga gaatacaaat
     1021 gagagcggca tttttgcttc tgctcgtctt cttcgcaatc ccctcgtttg cggaagcctc
     1081 gggctatctg agaaacggga acgggcatac ggtggcgctg accttcgacg acggcccccg
     1141 tccggggtac gtcgagccga tactggctat tctgcgggag aaggacgtca gggcgacgtt
     1201 cttcctggtc ggacggtacg ttctggagca tccggagctg gtgcgggcga tcgacgcgga
     1261 ggggcatacg gtggcgaacc atacgttcta ccacaacaac ctgacgtcgc tgccgccgga
     1321 aaacgtctac cgcgagtggc ggctctgcaa cgaggccgtg gagcgggtta ccggcaaaaa
     1381 accgcggttc gcccggccgc ccggggggcg ctacaacact ttcgtcgcgg agcgggcgaa
     1441 tgaagagggg ctgcgcatcg tcctgtggac caacaatccg ggggactacg ccgcgtcgct
     1501 cgacggcccc tctctcgccc ggaaggtgct ctccagggcg aaagagggcg acatcgtgct
     1561 gctgcatgtc ggcgtcgcgc ccacgatcga cgcgctgccg gaaatcatcg acggctaccg
     1621 taggaaaggg ttctccttcg tcaccgtcga cgagatgcgg taacttcgcc tacttctgac
     1681 cggcggggaa cttctgaatc gtccacccgg cggcggaggg gaagaaggag gccagcgctt
     1741 cggaaggctc ctccgcgacg cacaccgtgg cgtcctccat ccggacgacg aggcggcaga
     1801 gccgctcctg ctcggcggat actgaaacgc cgaagggccc tttgacgagc atcggcgtcc
     1861 ctccttcggc gacgatctcc tcgcggatgc gctcgctctc ccagtgcacc agcgcggcga
     1921 cgttcagata cttgccggag gcgagccggg cggactccca ctcgcggctc agcatcgcgg
     1981 ccgacgcggt gcgggcgaga atctccagcg tttccagatc catggtgaca gtacccggaa
     2041 gcggctccga taccgcgata aaggcgataa ggcggtcgcc gtcccagagg gggaagatcg
     2101 tcttcattcc ttccattgcg tcgagcgacg cgaggaaggg aacctccata aaggcgaaaa
     2161 gccgcttccc ctcctccatc gtgaaggagg acgggcggtc gccttcaaga atgccgatgg
     2221 ggaagcccgc cggacgcccg gtacgctgca ggatgccgtc ctccttcgag gcgaagacga
     2281 cgtagccgcc cgcgtgcggc tgccaggcca caagcaccgc gcgcggagcc tgggtcatct
     2341 cggagaagat gtcgagtatc tggagcagca gctcttcctt cgacgtctgt tcaaggaatt
     2401 tgagggccgc ctcgtggagc gcgttcagca ggaaggctct ccgtgaaagc tcctgcatgt
     2461 tccgctcctt ctcctcccgc atcgcggaca tggagagcgc cttcgatgcg acattcccca
     2521 gcagctccat gaagttcagg gcttcctcgc cggggagcgt ctcccagcgg agaaggcaga
     2581 agagcctgac gtccccgccg atgatcggga gaatggcgga ccactcgccc gcgaggtcgt
     2641 ggaagaccgc ttcaccaatc gagtcgcgca tggactcctc cagccgcacc ggaacgggag
     2701 agagaatgtc gcgcgcgaag atccctttgc ggggaggcat ctgtatatcc tcgccgtcga
     2761 ggtgcgagag acgcccgcct ttatccagaa cgacggagag acgcgaagga aacaggctct
     2821 ccgtgagcac gtcgcagaga aacgcgccga agtagtccag ccccatcggt tcgaagaccg
     2881 acgcgagcgt gctcttggtg gccagaacca tgtacgccag cttggagaga cgctcctccg
     2941 ttccggcggt cttctggtgt ttctgccaga ggctgagcag tccggcgaat gaaggcatat
     3001 ggtcgatcag ctccttcggc ggctcttccg aaaggaggat gaaaaagacc cagtctctcc
     3061 cgcggagacg aatcccccac ggccactccc ccgggagggg ggccgccgcc acgtcgtacg
     3121 cctcgcgaag gaagggggac ggaaacgcgt tttgaatatc acgggctctt cctttaagga
     3181 aaagactctc cacccctgcg ccgaaggctt cgagaacggt caggtcctct ccctccggcc
     3241 ggaggatcac ccctcgcgcc gcatgcgcgg cgaggctgtc ggaaagcagc gtgaggacgc
     3301 ctccgttcaa aagaaattcc gtccgttcaa aaagatcctt catgaaccat caacatccct
     3361 tcattggtgc ggatatacac gatcccgtgc ccgggttccc ctgtcccaag ttcggggcga
     3421 cgctccttct gtagtcagga agactcctct atggtatctt aaagttgcaa aagacgccac
     3481 aaaaaaaatc cggggacgcc gggggcgttc ttcgacaaaa cacagggtga attatgcatt
     3541 cttccgatac gacgaaaaaa cagcccaaaa aaatagcggc gcccggacag gagagccgcc
     3601 gttttgtccg ggtggagtgc gtcctccgcg cggagtttcg gctgcacaat catgacgcct
     3661 ggctgcctgc cgaagtgctc gacctgagcg tcgccggcat gaagctcagg ttcaacccct
     3721 tccagagagg gagaacgctg ctccccgaag tatttgagtg ggccgacagc cgtttccgct
     3781 ttcccctgaa acgagagttt tttcatctcg acgggcattt tctgaagatc taccagcgga
     3841 gcgcaggcct cttcacggca ggagtggagt tcgacgaacc ttcgccggag gaccagttca
     3901 agctcgtcga gctctatgcg gagttccgga gacggagagg cgcgatctga ttccccttct
     3961 tcctttagcg ctgcgcgagg ggcgcttgct ttatggcaat aaaaagagta gcgtattctc
     4021 tattcttttg gaggagatga tgtgatgaaa ctgaatcggc tgggttcccg cattctcgtt
     4081 gtcgtgctcg cgctgctgat cgtatcagtc ggcggcatag gattttacgc catgaagatt
     4141 gcgggcgaca acacgcgccg gatggtgaac gactatgaaa tctccgtagc caacggactt
     4201 gcgcacgaac tggacgcggt cttcaaccgt ttcgacggca tactgcaagc gctttcctcg
     4261 cttgtgagca cccggttcac tccggcgatg ctcaacgccg acgtggccga cgtcgtggtc
     4321 cggcagttcg caagccagaa cggcaagttc gtcgcagatc tgggagcgca gtccgtcgaa
     4381 gcgcgcagcc tcttcatgat attcaacccc gagatcttcg gcgtcaagaa ggtcttcagc
     4441 gtggggttcc aaagaaaaga ccaggactcg ctctacatgt tcatggacag cgagcagttt
     4501 tccgccgcca aactcatcga ccggaaggag ccctcgacct cgtggttctg gacgccgctc
     4561 gacacggggc gtccctattg gggggatctc acccgctccg aaacggggga ggatctcgtc
     4621 acctacacta tgccgatcat catcggtgat aaaatcgccg ccgtcgccgg catgtccttc
     4681 gatttttcgt ttgtccgcac gatgctggcg tcggtaaaaa tctacgacac ggggtaccca
     4741 ttcctgatga accgcgatct ccgcttcctc taccacccct cgctctcgtt cgacggcccc
     4801 gatctgcgaa aggtatcgga cggttccatg gcctcctttg ccgacgccat gctgaaagag
     4861 ccgagcggac gcttctcgta cttctatgaa ggagatgaaa aaacgctggc gtacaagagc
     4921 ctcgccaacg ggtacatcgt cgcggcagcc gcgacgaccg ccgagtcgct cgccgcggtc
     4981 ggcgcgatgc agcgcgccgt ctacataggc atcgccgcgg tgctccttgt cgccgtcgtc
     5041 gtcgtgatcg tcttctcccg cagcctcgca aagcccctcc aggccgtggc cgcaggcgcg
     5101 cggaacgtcg ccgaaactgg cgaccttacg aagacgctct ccgtcaagac ctccatcgag
     5161 gagatcaagg acgtcgcctc cgccgtcaac gacatgatcg gcggcacggc cgaagcagtc
     5221 cggaacatca tcgacaactc gcgcaaggtg ctcgcccgcg cggaggacat gagcgccgcc
     5281 tcggagcaga gcaccgcctc cgtccaggag gtcatcaccc tcgtcgggaa ggtcgcgcgg
     5341 aacacgcagg actccgcgag cgccattgaa gaagcgaacg ccggaatcga ggaggtctca
     5401 agcggagcgc aggccggagc gcaggccgcc gtcgagacgg gcgagcgcgc gcaggagatc
     5461 tcccatgccg ccgagaaggg cgggaaggcc ctcgacgaca tggcggggct gatcgaaaag
     5521 gtctccaaat ccggcgaaca ggtgagcgcc gccgtcggcg acctcgcaaa gtcggtgtcg
     5581 gggatcacgg gattcgtcaa caccatcacg cagatcgccg accagacgaa cctgctggcg
     5641 ctgaacgcgg cgatcgaggc ggccagagcg ggagaggcgg gacggggctt cgcggtcgtc
     5701 gccgaggagg tcaggaagct ggccgaggag tcgaaccggg ccgcgggcga agtggggcgc
     5761 gtgatcgggg agatctcggg gaagaccgag aacgccatga aggaccagaa gggaagcgcg
     5821 gagcagatca agcagctcgt tgcgcgggcg aaggagacga agaccgtcat cgacgacgtg
     5881 gtggtgaagg tg
//
LOCUS       DAOSWW010000040         5884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_8960_length_5884_cov_5.220106, whole genome shotgun sequence.
ACCESSION   DAOSWW010000040 DAOSWW010000000
VERSION     DAOSWW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8960_length_5884_cov_5.220106"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PK442_01555"
     CDS             <1..587
                     /locus_tag="PK442_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOO86362.1"
                     /translation="SMDEALALPSEKSVMIALRTQQIIAYESGVTNTVDPLAGSYVVE
                     SLTNEIEEKAFEYIQQIDEMGGMLTAIEKGYVQKHIQDAAYEFQRAVESKENVVVGVN
                     KFQIEEKNKDMKLLTVDASVGERQIKKLQELKASRDNVAVSNALDAIRSAALNESQNL
                     MPLIIDAVRVYATEGEICGVLRGVFGEYTENIVL"
     gene            638..1051
                     /locus_tag="PK442_01560"
     CDS             638..1051
                     /locus_tag="PK442_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOO86363.1"
                     /translation="MDERKIRVVVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     AEQIVETVLQEDADAVGISILSGAHTFYFTKIIDMLREKDAGDVIVFGGGVIPDSDIP
                     GLIEAGAGAVFGPGTPTTVCVEWLEGAVAKKRAKE"
     gene            1062..2012
                     /gene="meaB"
                     /locus_tag="PK442_01565"
     CDS             1062..2012
                     /gene="meaB"
                     /locus_tag="PK442_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOO86364.1"
                     /translation="MDTLLEKALGGDIRSIARIISLIENDNPEKDALLKRVYPMTGKA
                     IVLGITGSPGAGKSTLTDKVIEHFRRQGKKVGVIAVDPSSPFSGGAILADRIRMQRHT
                     GDSGVYIRSMGTRGSLGGLSRGTRESVILLDACGYDVIIIETVGVGQSEVDIIKIADT
                     VCLVLVPGMGDDIQIMKAGIMEIANVFAINKADKPGVDRVAAEVRLMLDLVKDRPWVP
                     PVHLTVAENGTGIEELVASIASHSVFLKSNPDGVRREWRRLAFEVEEILLQHITRMTE
                     KSWNAYKTDEVMELLLSRKSNPYAVAEDVLRKIIPGTRVV"
     gene            2040..3599
                     /locus_tag="PK442_01570"
     CDS             2040..3599
                     /locus_tag="PK442_01570"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOO86365.1"
                     /translation="MSQRTIEELCEELLLKREKAQHGGGEKAVAKQREKGKGTARERI
                     EKLLDPGTFIEVDEFVRHRCVNFGLEKTVFDGDGVIIGWGLVEGRKVYVYSQDFTVLG
                     GSLGEMHAAKICKIMDMAMSTGCPVVGINDSGGARIQEAVDSLNGYGAIFSRNTIASG
                     VIPQLSVIAGPTAGGAVYSPALTDYIFMVEGTGIMHITGPAVIKAVTGEEVTSEQIGG
                     SMANNSKSGNAHFSAKSEDECIAQVRKLLSYLPQNNLEEAPEKETGDDPLREDAELRN
                     IVPVNPNKGYDVREVIRRVVDESDFFEVQPLWAKNILTGYARLGGKVIGIIANQANHL
                     AGCLDIDASDKASRFIRHCDAFNIPVVTFVDVPGYLPGTAQEFGGIIRHGAKMLYAYS
                     EATVPLITVVLRKAYGGAYLGMCSKALGADIVLAWPQAEIAVMGAEGAANIVFRREIE
                     AAEDSVATRTRKIEEYREAFANPYVAAERGLVDRVILPEATRGEIWRALVGAESKRDL
                     RPRRKHGVMPH"
     gene            3625..4107
                     /locus_tag="PK442_01575"
     CDS             3625..4107
                     /locus_tag="PK442_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOO86366.1"
                     /translation="MSIAPAATAVTETVDAAAAAAASASARIAEEMSGVGGALTMSII
                     AFSVVFLVLGGLSGVIYAIKYMAQGLERKKNEPPAGNAAAGNAAAPAPVGAAPAAQSG
                     ADGRLMAVLAAAVAAAGIGGRITGVTVAGGSRARPARAAGWRSASLAEGIQSLSRDWK
                     "
     gene            4299..4706
                     /locus_tag="PK442_01580"
     CDS             4299..4706
                     /locus_tag="PK442_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOO86367.1"
                     /translation="MSKKYRITVDGTAYTVEVEDLGAGAPAASAPAAAAAPAPAPVAA
                     PAPAAAPAPAAAAPAPAAGGAGVPVTAPMPGKVLKVTVSAGAAVKNGDLVLVLEAMKM
                     ENEIFSPADGVVKEIRSREGDAVNSGDVMMVVG"
     gene            4763..5884
                     /locus_tag="PK442_01585"
     CDS             4763..5884
                     /locus_tag="PK442_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOO86368.1"
                     /translation="MEIYMQAIREIIVSSGFAGLTGGHLMMIVVALVMLYLAIGKGFE
                     PLLLVPIGFGCLLVNLPLSGIMDDGGFLRYVFFGTEHEIYPVIIFMGIGALTDFAPLL
                     ANPITFLLGAAAQFGVFIALFGAMLMGFSVNEAASIAIIGGADGPTSIYLTMKLAPQI
                     LGAVAVAAYSYMSLVPLIQPPVIKMFTTKADRKIRMDTLRPVTKLEKILFPIVTTMAA
                     GLILPASVPLVGILMFGNLVRECGVTERLSQAAQNEILNTTTIFLGLSVGATMNAEEF
                     LTAATLKIILLGLVAFIFSTAGGVLFGQLMKKWSGGKINPMIGAAGVSAVPMAARVVQ
                     RVAQKESPGNFLLMHAMGPNVAGVIGTAVAAGVMLTLLS"
BASE COUNT         1432 a         1648 c         1814 g          990 t
ORIGIN      
        1 acagcatgga cgaggccctc gcgcttccga gcgagaagag cgttatgatc gcactcagaa
       61 ctcaacagat cattgcttac gaatccggag tcaccaacac ggttgatcct cttgcaggaa
      121 gctacgtggt tgaatccctc acgaatgaga tcgaagagaa ggcgttcgag tatattcagc
      181 agatcgacga aatgggcggc atgctgacgg cgattgagaa ggggtatgtc caaaagcaca
      241 tccaggatgc agcgtacgaa tttcagcgtg ctgtggaatc aaaagaaaat gttgtcgtcg
      301 gagtcaacaa gttccagata gaggagaaga acaaagatat gaagcttttg actgtcgacg
      361 cctccgtcgg agagcggcag atcaagaagc tgcaagaatt gaaagcctcc cgtgacaatg
      421 ttgccgtgtc caacgctctt gacgccatcc gctcagccgc tttgaacgag tcgcagaatc
      481 ttatgccgct tattattgac gcagtccgtg tctatgcgac tgaaggtgaa atctgcggcg
      541 tactccgcgg cgtcttcggc gaatatacag aaaatatcgt tttgtagcct tttaattaag
      601 gctgtgcatc aggattatca taatggaggg atcttccatg gatgagagaa aaatacgggt
      661 tgtggtggcg aaaccgggtc ttgacggcca tgacagaggc gcgaaggtcg tagcgcgcgc
      721 gcttagagac gcaggaatgg aggtcattta taccggtctt cgccaaaccg cggagcagat
      781 cgtggagact gtgctgcagg aagacgccga cgcagtggga attagcatcc tctccggagc
      841 tcatacgttt tatttcacca agattatcga catgctcagg gagaaggacg ccggagatgt
      901 tatcgtgttc ggcggaggag tcattccaga cagcgatatc cccggtctta tagaggccgg
      961 cgcgggcgcc gtttttggtc ctggaacgcc gacaactgtt tgtgtcgagt ggcttgaagg
     1021 ggcggtagca aaaaaacggg cgaaagagta gtccattggc tgtggatact ttgctcgaaa
     1081 aggcgcttgg aggagacatc cgctctatcg ccaggattat cagcctgatc gaaaacgaca
     1141 acccggagaa ggatgccctc cttaagcgtg tatacccgat gaccggcaaa gcgatcgttc
     1201 tcgggattac aggcagtccg ggggccggga aaagtacgct cacggacaag gtaatagagc
     1261 acttccgccg ccaggggaaa aaagtcgggg tcatcgcagt tgatccgtcg agtccctttt
     1321 ccggaggcgc tatccttgcg gacagaattc ggatgcaacg gcatacaggc gactccggag
     1381 tatatattcg gagcatgggg acccgaggtt ccctcggcgg actcagcaga ggaacaagag
     1441 agtccgtaat tctacttgat gcctgcggct atgatgtcat tatcatcgaa accgtcggcg
     1501 tagggcaatc ggaagtggat attatcaaaa ttgccgatac ggtctgtctg gttctagttc
     1561 ccggcatggg cgacgatatc cagatcatga aagcgggtat catggagata gccaacgtct
     1621 ttgccataaa caaggcggac aaaccgggag tggacagagt cgccgccgaa gttcgactga
     1681 tgcttgatct cgtaaaggat cgcccgtggg ttccgccggt gcacctgact gttgcggaga
     1741 acgggaccgg aatagaagag ctggttgcaa gtatcgcgtc acacagtgtt ttcctcaaaa
     1801 gcaatcctga cggagtgcgg cgtgaatggc ggcgtctcgc ttttgaagtc gaagagattt
     1861 tactgcagca cattacaaga atgacggaaa aatcttggaa cgcgtacaaa acagatgaag
     1921 ttatggagtt gttgctttcg agaaaaagca acccttatgc tgttgcagag gatgtattga
     1981 gaaaaattat tcccgggacc agagtggtct gaggggaacg aagggggcag attcatcaga
     2041 tgtcgcagcg aacgatagaa gagctctgcg aggagctgtt gctgaagcgt gagaaagcgc
     2101 agcatggagg cggtgagaaa gcggtcgcca agcagcgtga gaaggggaag ggaacggcgc
     2161 gggagcgtat agagaagctg ctcgaccccg gtacgttcat cgaagtggac gaattcgtcc
     2221 ggcaccggtg cgtgaacttc ggactggaga agacggtctt cgacggagac ggagtcatca
     2281 tcgggtgggg actggtggaa gggcgcaaag tgtacgtcta cagccaggac ttcacagtac
     2341 ttggcgggtc gctcggggag atgcacgccg cgaagatctg caagataatg gacatggcca
     2401 tgagcaccgg atgtcccgtg gtcggcatca acgacagcgg cggagcgagg atccaggaag
     2461 ccgtcgactc gctcaacggg tacggagcga tcttctcccg gaacaccata gcgagcggcg
     2521 tcatcccgca gctctccgtc atagccggcc ccaccgccgg aggcgccgtg tacagccccg
     2581 cgctcaccga ctacatcttc atggtcgaag gaacgggaat catgcacatc accggccccg
     2641 ccgtcatcaa agccgtcacc ggcgaagaag tcaccagcga acagataggc ggctccatgg
     2701 ccaacaacag caagagcggg aacgcgcact tcagcgccaa gagcgaagac gaatgcatcg
     2761 cgcaggtcag gaagctcctg agctaccttc cgcagaacaa tttggaagaa gcgcccgaga
     2821 aagagacggg agacgacccc ctcagagaag acgccgaact gaggaacatc gtccccgtca
     2881 accccaacaa agggtacgac gtgagggaag tcatccggag agtcgtcgac gaaagcgact
     2941 tcttcgaagt ccagccgctg tgggccaaga acatcctcac cgggtacgcg cgtctcggag
     3001 gcaaagtcat cggcatcatc gccaaccagg ccaaccacct cgccggatgc ctcgacatag
     3061 acgcctcgga caaagccagc cggttcatcc gccactgcga cgccttcaac atccccgtcg
     3121 tcaccttcgt cgacgtcccc ggatacctcc ccggcacagc gcaggaattc ggaggcatca
     3181 tccgccacgg cgccaaaatg ctctacgcgt acagcgaagc caccgtcccc ctcatcaccg
     3241 tcgtattgag gaaagcgtac ggaggcgcgt acctcggcat gtgcagcaaa gccctgggag
     3301 ccgacatcgt cttggcatgg ccgcaggccg aaatcgccgt catgggagcc gaaggcgccg
     3361 cgaacatcgt cttccgacgc gagatagaag cagcggaaga cagcgtcgcc acgcgcacca
     3421 ggaaaataga agaataccgt gaagccttcg ccaaccccta cgtcgccgcc gaacgaggcc
     3481 tcgtcgaccg tgtcatcctg cccgaagcca cgcgcggcga gatctggaga gcgctcgtag
     3541 gagcggagag caagcgcgac ctgcgcccgc ggcgcaagca cggcgtcatg ccgcactaaa
     3601 taccaaggaa ggagtgacgc acgaatgagc atcgcacccg cagcgaccgc cgtaaccgaa
     3661 acagtggacg cagccgccgc cgcagccgcg tcagcctccg cgcgcatagc ggaggagatg
     3721 agcggagtcg gaggagcgct caccatgtcc ataatcgcct tcagcgtcgt ctttttggta
     3781 cttggagggc tgagcggagt catctacgcc atcaagtaca tggcgcaggg acttgaacgg
     3841 aagaagaacg aaccgcccgc aggaaacgca gcggcaggaa acgccgcagc gccggcgccc
     3901 gtcggcgccg cccccgcggc gcagagcgga gccgacggtc gtctcatggc agtgctcgcc
     3961 gccgcggtag ccgccgcagg aatcggcggc cgcatcaccg gagtgacggt agccggaggc
     4021 agccgcgccc gccccgcccg agcggccgga tggagaagcg cctcgctcgc cgaaggaatc
     4081 cagagcctct cccgggactg gaaatagcgc cgcagccgcg cgcacgcccg gagaagcgaa
     4141 gaagcaataa agaagcaata aagcagcaat taaaaagtat tagaagtctt aaaagcatta
     4201 aatagcaagc gaaaagcaaa aaagccaaga gacgaataaa gaacggcaac acacagagaa
     4261 cactcgaaac caaacgaaag aaggaggctc atcgcacaat gagcaaaaaa tacagaatca
     4321 cagtcgacgg aacagcctac acagtagaag tggaagacct cggagcgggc gcccctgcgg
     4381 cgtcggcccc ggcagctgcg gcagctccgg ccccggcccc ggtagcggcg cccgcaccgg
     4441 cagccgcccc cgcccctgcg gcggcagccc ccgcgcccgc agcaggaggc gccggagtcc
     4501 ccgtcaccgc ccccatgccc ggcaaagtcc tcaaagtaac cgtaagcgcc ggagccgcgg
     4561 tcaagaacgg ggacctcgtc ctcgtcctcg aagccatgaa gatggagaac gaaatcttct
     4621 cccccgccga cggagtcgtc aaggagatcc ggagccggga aggagacgcc gtcaactccg
     4681 gcgacgtcat gatggtcgta gggtagcccc gggaccagga cacaacctgt agagacagga
     4741 gcaaggaggg agaccgaggg caatggaaat ctacatgcaa gcgataagag agatcatcgt
     4801 atcgtccgga ttcgccggac tcaccggagg acacctcatg atgatcgtcg tggcgctggt
     4861 gatgctctac ctcgccatag ggaaaggatt cgaaccgctg ctgctcgtac cgatcggatt
     4921 cggctgtctg ctggtgaacc tgccgctgtc ggggataatg gacgacggag ggttcctgcg
     4981 gtacgtcttc ttcggaacgg aacacgaaat ctaccccgtc atcatcttca tgggaatagg
     5041 cgccttgacc gacttcgcgc cgctgttggc caaccccatc acgtttttgc tcggagcggc
     5101 ggcgcagttc ggagtcttca tcgcgctctt cggcgccatg ctgatgggat tctccgtcaa
     5161 cgaagccgcg tcgatcgcca tcataggagg agcggacggc cccacgagca tctacctcac
     5221 catgaagctc gcgccgcaga tcctgggagc tgtggctgtg gcggcgtaca gctacatgtc
     5281 gctcgtgccg ctcatccagc cgccggtcat caaaatgttc acgaccaagg ccgaccggaa
     5341 gattcgaatg gacacgctgc gtcccgtcac gaagctggag aagatcctct ttccgatagt
     5401 gacgacgatg gcggcgggcc tgatcctccc cgcgtcggtg ccgctggtgg gcatcctcat
     5461 gttcgggaac ctcgtgcgtg aatgcggggt cacggaacgg ctgagccagg cggcgcagaa
     5521 cgagatcctg aacaccacga cgatcttcct gggactgtcg gtgggagcga ccatgaacgc
     5581 cgaggagttt ctgacagcgg cgacgctgaa gatcatattg ctgggacttg tagcgttcat
     5641 attcagcacg gcgggcggcg tcctgttcgg ccagctgatg aagaagtgga gcggcgggaa
     5701 gatcaacccg atgataggcg cggcgggagt ctcggcggtg ccgatggcgg cgcgcgtcgt
     5761 gcagcgagta gcgcagaagg agagccccgg gaacttcctg ctgatgcacg caatgggccc
     5821 gaacgtcgcc ggagtcatag gcacggccgt cgccgcaggc gtcatgctta cgctgctgag
     5881 ctga
//
LOCUS       DAOSWW010000041         5788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_9188_length_5788_cov_3.703646, whole genome shotgun sequence.
ACCESSION   DAOSWW010000041 DAOSWW010000000
VERSION     DAOSWW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9188_length_5788_cov_3.703646"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1099)
                     /locus_tag="PK442_01590"
     CDS             complement(131..1099)
                     /locus_tag="PK442_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO86369.1"
                     /translation="MYEWRRQIQALVDAIDECIKNHDDEALTLRSLSCGLGYSEFHTT
                     RKFKEISGMQFREYLRGRRLAFALKEVRDSGKSLLDIAFDYGFSSHEAFTRAFKGTYG
                     VAPGEYRKNPKPVVLRTKINPFDRYFLGLGEIGMMKSADDVKIYCVTIPAHKFVHIKN
                     YESNGYWDFWKKQSNIPGQDCETICGLLDSIKGKLDDDGGSESDSGSGQIMAYINDPD
                     GRLCDWGIPRTECYGARLPLDYKGEVPPQMLLIDIPEAEYIVFEHGPFDYEQENRSVE
                     EKIEHAMAAFDYADAGYLLDTSPGRITYFYHNPGQFFKYVRPVRRV"
     gene            complement(1184..1636)
                     /gene="alaE"
                     /locus_tag="PK442_01595"
     CDS             complement(1184..1636)
                     /gene="alaE"
                     /locus_tag="PK442_01595"
                     /inference="COORDINATES: protein motif:HMM:NF018333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034639 - L-amino acid efflux
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HOO86370.1"
                     /translation="MTGTPGVNNKRKQISFLIADITAMILFSTGLCMIIEIFVAGLSF
                     FQSVGARIAAIPVNLITGRPYGWFRDTLFLTLGIDRSSPVKLFFGDTLAFVVFQVPLY
                     VLVLLFAGATWKQIAVTSLSASLVFSLTGRPYGIFLDFCRRIAVKLLG"
     gene            1894..2055
                     /locus_tag="PK442_01600"
     CDS             1894..2055
                     /locus_tag="PK442_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86371.1"
                     /translation="MISSKKAVFFLCVDPIDHVASWIPGDLVRYVRESKMQGEDASRA
                     HPPLEEPPL"
     gene            complement(2157..3443)
                     /locus_tag="PK442_01605"
     CDS             complement(2157..3443)
                     /locus_tag="PK442_01605"
                     /inference="COORDINATES: protein motif:HMM:NF018234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO86372.1"
                     /translation="MKHRPSTLMAIFIFLLSASLLQSRAALATEEPLAVSDMHATAFH
                     YFFQDGDMDFHFGNLILGSAVHGGAGIGEAFYAASRIQDGDAGDWQREWAELAGRVEA
                     RGETALAAGHLVSARDQLLRAAYYYRLSLVSMLPDNPAFKERGGKVRELFQKGGALFD
                     PPVEYFEVPFEGTSLPGYFRKAASGDRPVKTLLMIGGGETFAEDLFFYIAHQAHARGY
                     NFATVDLPGQGLLPLEGMVFRTDTQAPVKAVVDVLVKRPDVDTERLSAFGISGGGLFV
                     PQAAMFDPRIKAVAMCSAVVDARPLFATMPAALDTPEERAGWTSFHEGVVKSICWRYG
                     VPMDHPEKLIEANEGNTFDPAKVAVPALIIVGEGEYRSLEVQRQQKIALDNFPNPSKK
                     MVVTPSDEGASNHCVMENRALIGQVLFDWLDETMGE"
     gene            3636..5054
                     /locus_tag="PK442_01610"
     CDS             3636..5054
                     /locus_tag="PK442_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO86373.1"
                     /translation="MKDVIGLGTILLWQWLTVLKGPLFPSLVDIPLLGPEGGFALFSL
                     ILFATFLLLALKGKDLPEAWFVRAVRLAPILMSAAWLPWGWRGASLPAPLLLFALVLP
                     SFGSAVHLASWQWRLSQAPFSRQAVSFGLACLVRSSAILLLSFAPRPLLHTVALLLPF
                     LGSFLWSPPGAVLPPDASRGRFRLSPRLAFRVASFFIVSAMFLSILLAQGSGEALAPK
                     VLTDPFYTLGALGAGLALLRWFAVDLRSIYFFAQSLLVLGFMAFAALGGGRPLIPLAL
                     LQLGFGTFGAYLFTLLLYLGSRAGRSEALSVVSSIQAVNSGSVVLGMLLTRTTGCAAG
                     LLEVPFVLAASLLGVGLLFFSALFLRDDPTSFAGFDLRGASDEEVPSKEAGAEPPGEE
                     AEPGEREEGVFLRFMEQGLSLQEIRVALLVDEGLSNADISQRLNITSNTLRSHLRKIH
                     KKIGSSSRKALREELQRMRGDA"
     gene            5330..5773
                     /locus_tag="PK442_01615"
     CDS             5330..5773
                     /locus_tag="PK442_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOO86374.1"
                     /translation="MNDTKIGVSGGRFLAFRLAPGEELLGALRRIVAANGIRAGYVAG
                     VVGSLSRACLRFAGRRDGTALDGCFEVVSLVGTLDAEGEHLHMAISNQEGTTTGGHVL
                     EGCVVRTTMELVLGDLSDLEFRREYCPLSTYEELAVMPRKNNKGE"
BASE COUNT         1076 a         1670 c         1712 g         1330 t
ORIGIN      
        1 ctatgaagcc cagctatcgc caatcgctct atagactgct cgaaaagaac agagagccat
       61 cggcctgcac ggagtgcgct ctttccatgc agaccgatgc ttgttgaccg cagcggacga
      121 gccgttgatt ctataccctc cgcacaggcc tgacgtactt aaaaaactgc ccgggattat
      181 ggtaaaagta cgttatcctt ccgggggagg tgtcaaggag gtatccggcg tccgcataat
      241 cgaacgccgc catcgcgtgt tcgatcttct cctccacgct ccggttttcc tgctcgtaat
      301 cgaacggccc gtgttcgaag acgatatact cggcctcggg aatatcgatc agaagcatct
      361 gcggcggcac ttctcctttg tagtcaagag gtaggcgcgc gccgtaacac tccgttcgcg
      421 gaatgcccca atcgcagagt ctgccgtccg ggtcgttgat gtacgccata atctgcccgc
      481 tgccgctgtc ggattcactg ccgccgtcgt cgtccaattt gcccttgatg ctgtcgagca
      541 ggccgcaaat cgtttcgcag tcctgccccg gaatgttgct ttgttttttc caaaagtccc
      601 aatagccgtt gctctcgtag tttttgatgt gcacaaactt atgcgcggga atggtgacgc
      661 agtaaatttt cacatcgtcc gcggatttca tcatgccgat ctctcccagt cctaaaaagt
      721 agcggtcgaa agggttgatt ttcgtgcgaa ggacgacggg cttggggttt ttccggtatt
      781 cgcccggagc gacgccgtac gttcctttga aggctctggt gaacgcttcg tgcgacgaga
      841 agccgtagtc gaacgcgata tccagaagac tcttcccgct gtcccgaacc tctttgagcg
      901 cgaaggccag tcttctcccc cgcagatact ccctgaactg catacccgaa atctctttga
      961 atttcctcgt ggtatgaaat tcggaatacc ccaatccgca cgaaagactg cgcagcgtca
     1021 aggcttcgtc atcatggttt ttgatacact cgtcgattgc atcaacgagt gcttgaattt
     1081 gtcgccgcca ctcgtacatt cgcccgttca ccccgttccg accaccctcg atgtattgta
     1141 aggagctggg gagccttccg cttgatttta cttgctgttc gtttcacccg aggagtttga
     1201 cggcgatccg cctgcaaaaa tccagaaata tgccgtacgg ccgtccggtc agcgagaaga
     1261 ccagcgacgc ggagagggag gtgacggcga tctgtttcca tgtggcccca gcaaagagca
     1321 ggacgaggac gtacaacggc acctgaaaga cgacgaacgc cagcgtgtcg ccgaaaaaga
     1381 gtttcaccgg actggacctg tcgatgccga gcgtcaggaa gagcgtgtct ctgaaccatc
     1441 cgtacggcct tccggtgatc aggttgaccg gaatagccgc gattctcgcc ccgaccgact
     1501 ggaagaacga gagccccgcg acgaagatct cgatgatcat gcagaggccc gtcgagaaca
     1561 agatcatggc ggtaatgtcg gcgatcagaa agctgatttg tttcctcttg ttgttcacgc
     1621 cgggggttcc ggtcatagaa ttcacctctt caaagttgtg agtgcagaag atttttgttt
     1681 ccttaaaaac aactgcattt ttctacaacg cctgtcattg tagcacgcgg aatgcggatc
     1741 gttttccaag agaagcggga aaatcatctc tctcgttcgt ggactgctta ccgcttcgcc
     1801 attacgcatc tggacgcctg cgagttgacg cgaagcgctc ttgcgggcga taatgccacg
     1861 aaagcagaga gtgcgaaaaa ggagggattc gttgtgatct cgtcaaaaaa agccgtattt
     1921 tttctgtgcg tcgaccctat cgatcacgtt gcatcctgga ttcccggaga cctcgttagg
     1981 tatgtccggg aatccaagat gcaaggggag gacgcctccc gggcgcaccc tccccttgaa
     2041 gaacctccgc tgtgaagcgg ggctcccccc gctctcttcg ctctccgggg agagtctccg
     2101 accatgggcc tgcgtgagcc gtgtccgcaa tggcgagccc gatacctgcg cctcgttcac
     2161 tcgcccatcg tctcgtcgag ccagtcgaaa agcacttggc cgatcagggc gcggttttcc
     2221 atgacgcagt gattgctcgc gccctcgtcc gacggcgtga cgaccatctt cttcgaggga
     2281 ttcggaaaat tgtccagagc gatcttctgc tgccgctgca cttccaggct ccggtattct
     2341 ccctcgccga cgatgatcag agcgggcacg gccactttcg ccggatcgaa ggtgtttccc
     2401 tcgttcgcct cgatcagttt ttcggggtgg tccatcggca ctccgtagcg ccaacagatg
     2461 ctcttcacca cgccttcatg gaaggaggtc caccccgctc gctcttcagg agtatccaag
     2521 gcggccggca tggtggcgaa gagggggcgg gcgtcgacca ccgcggagca catggcgacg
     2581 gccttgatgc gcgggtcgaa catcgccgcc tgcggaacga agagtccccc tccgctgatg
     2641 ccgaaggcgg acagacgctc cgtatccacg tccggcctct tgacaaggac gtccacaacg
     2701 gccttcacgg gcgcctgcgt gtccgtgcgg aagaccattc cctccagagg aagcagtccc
     2761 tgtccgggaa ggtccaccgt ggcgaaattg tagcctcggg cgtgggcttg gtgagcgata
     2821 taaaagaaga gatcctccgc gaaggtctcg ccgccgccga tcatgagcag ggtcttgacg
     2881 ggacggtcgc cggaagcggc ttttcggaag tatccgggaa gcgacgtacc ctcgaagggg
     2941 acttcgaagt actccaccgg aggatcgaac agcgcgccgc ccttttggaa gagttcccgg
     3001 accttgcctc cccgctcttt gaaggcggga ttgtccggca gcatggagac cagcgagagg
     3061 cgatagtagt aggcggctct caagagctga tcacgggcgc ttacgagatg ccccgcggca
     3121 agggctgttt ctcccctggc ctctactcgg ccggccagtt ccgcccattc gcgctgccaa
     3181 tcgcccgcgt ctccgtcctg aatacgggac gcggcgtaaa acgcttcgcc gatcccggcg
     3241 cctccgtgta ccgccgaacc aagaatcagg ttgccgaagt ggaagtccat gtcgccgtcc
     3301 tggaagaaat agtggaacgc cgtggcgtgc atgtcggata cggccaacgg ctcctcggtc
     3361 gcaagggcgg cgcggctctg caagagactt gcgctcaaaa gaaagatgaa aatggccatc
     3421 agcgtgcttg gtcgatgttt catggcaatc actccttttt gtatctatag tgagccatct
     3481 ccttgcggag agaactctca gggaaaagat tctacatcgg tagccccgcc cgcgggaggg
     3541 tgaattctct aaccctcttg aagaaaatag tctttgcaag gcttgactgt tcatatcttt
     3601 tctgttgtta tcttggaaaa gaaggaggtg gtggaatgaa ggatgtgatc ggcctcggaa
     3661 cgatacttct ctggcagtgg ctcacggtgt tgaaggggcc gctcttcccg tctcttgtcg
     3721 atatccctct tcttggtccg gagggcggat tcgcgctctt ctccctgatt ctcttcgcga
     3781 catttttgtt gcttgccctg aaggggaagg atcttcccga ggcgtggttc gtccgggcgg
     3841 tacgtctcgc cccgattctt atgtccgcag cgtggcttcc ttggggctgg cggggagctt
     3901 cgcttccggc tcctctgctc ttattcgcgc ttgttcttcc gtccttcggg tcggcggtgc
     3961 atctggcgag ctggcagtgg aggctctcgc aggcgccgtt ttcgcgccag gcagtctcgt
     4021 tcggactggc atgtctggtc cgaagctccg ctattctctt actctcattc gctccccgac
     4081 cgctgctcca tactgtcgcg ctgctccttc cctttctggg gagctttcta tggagccctc
     4141 cgggagcggt tcttccgccc gacgcttctc gcggcaggtt ccgtctctcg ccccggctgg
     4201 cgttccgtgt ggcttccttc ttcatcgtat cggcgatgtt tttgtcgatt cttctggcgc
     4261 agggaagcgg tgaagcgctc gcgccaaagg tgctgacgga tcctttctac acactggggg
     4321 cgctcggagc gggacttgcg cttctccgct ggtttgccgt cgacctgagg agcatctact
     4381 ttttcgcgca gtccctgctg gtgctggggt tcatggcgtt cgcggcgctc ggcggcgggc
     4441 gtccgctgat tccgctcgcc ctgttgcagc tcggcttcgg aacgttcggc gcctacctct
     4501 ttacgctgct gctctatctt gggtcgcgcg ccggaagatc ggaggcgctt tcggtggtga
     4561 gctcgataca ggcggtgaac agcggctcgg ttgttctggg gatgctgctg acccggacga
     4621 caggctgcgc ggccggtttg ctcgaagtgc cgtttgtcct tgctgccagt cttctgggcg
     4681 tagggctgct ttttttctcg gcgctctttc tccgggatga tccgacgagc ttcgcgggct
     4741 tcgatctccg tggcgcttcg gatgaagagg tcccgtcgaa ggaggcaggc gcggagcctc
     4801 cgggagagga agcggagccc ggggagcgcg aagaaggtgt gttcctgcgt tttatggaac
     4861 aggggctctc gcttcaggag attcgggtgg cgctgcttgt cgacgaagga ctttcgaacg
     4921 cggatatctc gcaacgcctc aacatcacat ccaacacgct gcggagccat ctgcggaaga
     4981 tccacaagaa gatcggttca tccagcagga aagccctccg tgaggaactt caaagaatgc
     5041 gcggagatgc gtaaagcggt gcggatgttg tgcggacgta tggtctctga aatcgtgatg
     5101 acgggcgatc cgcccgcaac aattcaactc aacagtatgt ccttcggact cccggtatgt
     5161 atcctcgccg ggagcaggct gtttgacacg ctttcttcga aacgcctttt ccttttcatc
     5221 ctttctcaca ccttgtcgca atatcttttt atgttataat tctcatcgct atttgtatgg
     5281 catattccgc gatgaccgcg gagaataaag gtattccagg gaggacgcaa tgaacgacac
     5341 gaagatcggc gtctccggag gtcggttttt ggccttccgg ctggctccgg gggaagagct
     5401 tctcggggcg ctgcggcgga tcgtcgccgc gaacggtatt cgggcggggt atgtcgccgg
     5461 ggtggtcggc agcctgtcgc gggcgtgcct gcgcttcgcg gggcgtcgcg acgggacggc
     5521 gctcgacggc tgcttcgagg tggtttcgct cgtcgggacg ctggacgcgg agggcgaaca
     5581 cctgcacatg gcgatctcga accaggaggg aacaacgacg ggggggcacg tactggaagg
     5641 gtgcgtcgtc cgcacgacga tggagctcgt gctgggcgac ctctccgacc tggagttccg
     5701 ccgcgaatac tgccctcttt ccacctatga ggaacttgcg gtcatgccgc gaaaaaacaa
     5761 taaaggggaa tgaacgatga agaacaga
//
LOCUS       DAOSWW010000042         5764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_9261_length_5764_cov_3.644071, whole genome shotgun sequence.
ACCESSION   DAOSWW010000042 DAOSWW010000000
VERSION     DAOSWW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9261_length_5764_cov_3.644071"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PK442_01620"
     CDS             complement(<1..1307)
                     /locus_tag="PK442_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOO86375.1"
                     /translation="MKLFSSIALLALLTLLLPRPARADEPLIPMEDFFRNPEQSGFQL
                     SPNGEYISFMKPWERRMNIFVQKIGAEEAVRVTSATERDIPAYFWSGNDRIVYLQDRG
                     GDENYRLYAVSSDGTESRELTPFENTRVTIVDSLEEQDDFLLIGMNRRDQRLFDVYRL
                     NLATGDLTLLEENPGDIAGWMTDHDGKLRVAVRSDGVNTSLLYRDKEDEPFRNILTTD
                     FRESVSPLFFTFDNKKLYVASNLGRDKSAIFVFDPQTVEHEELLFEHPDVDVSSLFRS
                     RKRKVITGTGFTTDRFRYHFFDEQRAALQKELEERLPDDEVVVASMSRDETKVLVYAG
                     SDRTRGTYFFFDRETKDFRKLAELAPWLPKERMAPMKHVSYTSRDGLTIHGYLTLPLG
                     KEPKNLPVVIHPHGGPWVRDGWGFDPQVQFLANRGLAVLQMNFR"
     gene            complement(1312..1725)
                     /locus_tag="PK442_01625"
     CDS             complement(1312..1725)
                     /locus_tag="PK442_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOO86376.1"
                     /translation="MKSHTEYLWFETKKRKELVHITPALQDILGRSGIGEGMMLVSAM
                     HITAGIIVNDNESGLHEDIMSWLERTAPEDPSYLHHRTGEDNGDAHLKRILIHHQAIL
                     PVTEGRLDLGPWERCFYAEFDGRRRKRVVVKVMGE"
     gene            1877..3937
                     /locus_tag="PK442_01630"
     CDS             1877..3937
                     /locus_tag="PK442_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86377.1"
                     /translation="MTEFSNDTLFDDGAAAMWRQDAGMLRAVLDVIPDIVSVQSPQLE
                     ILFYNKAGLDFLHASSEGTIGRNCYALLGRKEPCDDCLSLKAIRTGNAQEGEKYVPEL
                     DVWFEVRAIPVKDDAGSVRCVVEQLRDCTARKRNEERLARVLEELEAKNAELAETSAT
                     ARMLALRAEESSRAKSAFLANMSHEIRTPMNGILGMVELLLDTGLSMEQRDFGETISS
                     SADHLMELLDDLLDFSKIEAERLTLESAPFNLQELVEDLTVQTAVKGREKNLDVYAVV
                     EPDVPAFFRGDASRLRQVLGNLLSNAVKFTDTGEISLAVSLAQDGILRTLLRFEVADT
                     GIGVPEEKKELLFRPFSQVDASITRRFGGTGLGLAISRGLVELMGGNIGMKDGQSGGA
                     LFWFEVPLDKIDHGDIEDLPVDPNLMNTPVLLVGGTPGWRRSVTTHLERWLCAVASAE
                     REGVEACLAGALDAERPFSVVLVDGSSVPEDNPFSPRALPERLRSVSSFYLLDYPSRR
                     WRRAALSDAGYADFVPKPVKRNALKNLLLSGTESAGRIYSENTGVAPAGRKAKVLLVE
                     DSEVNRKVASVMLQRLGCSVSAAHDGFEALDFLRREAFDLVFMDVQMPVLDGIDAVKR
                     IRSGYGEIGRPDIPVIAMTARTLPGDREECFAAGMDDYIAKPVKMNVLEEILRKWLPG
                     GEAQ"
     gene            3934..4458
                     /locus_tag="PK442_01635"
     CDS             3934..4458
                     /locus_tag="PK442_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HOO86378.1"
                     /translation="MNSGERRMRIREQLALAGGPVKGAMLASLFSVSRQIIVQDIAVL
                     RAEGASIVATPQGYLLLPGRVSGMLTKTVVTRHSDSSSMEEELRIMVDRGARVLNVIV
                     EHPLYGEIAGNLMIFTRSDVDRFMEKMRESDGEPLSTLTGGVHLHTLEVPDETTWSSV
                     LDGLREKNYLLEEK"
     gene            4511..5557
                     /locus_tag="PK442_01640"
     CDS             4511..5557
                     /locus_tag="PK442_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOO86379.1"
                     /translation="MNETLSSAEEVGRKGAFRGYLVKVSNGMALGLFASLIIGLILRQ
                     AGGLLSMPSVELFGRIAQLLMGPAIGAGVAFAVGAPPLGLFSSLIAGAVGAGTFSFEG
                     GAVAVAIGEPVGALVASLAGAEFSKRIAGKTGMDIIVVPAGTILVGGLAGVVAGPFIA
                     GMMYWIGNAINVATTLHPIPMGILVSVMMGVALTLPISSAALSISLGLSGLAAGASTT
                     GCCCQMIGFAVASYRDNGIGGLFSQGLGTSMIQIPNIIRNPWIWIPPTLASAVLGPVS
                     TVFFRMENSRVGAGMGTSGLVGQVSTFETMGVAAWPGVLLLHFLLPAALSLLFAELLR
                     KTGRIRPGDMKLEK"
     gene            complement(5561..>5764)
                     /locus_tag="PK442_01645"
     CDS             complement(5561..>5764)
                     /locus_tag="PK442_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOO86380.1"
                     /translation="FGVALTVAISVVGVVMLGNLVGALLPFAAKKLKLDPAIMSGPFI
                     STVVDILGLLLYFEVARRVLHLG"
BASE COUNT         1072 a         1662 c         1864 g         1166 t
ORIGIN      
        1 cggaagttca tctgcagcac tgcgagcccc ctgttggcga ggaactgtac ctgcgggtcg
       61 aacccccatc cgtcgcgtac ccacgggcct ccgtgcgggt ggatcaccac ggggaggttc
      121 ttcggctcct ttccgagagg gagcgtcagg tagccgtgga tcgtcagccc gtccctcgat
      181 gtgtaggaca catgcttcat cggcgccatg cgctctttcg gcagccatgg cgccagctcc
      241 gccagcttcc ggaagtcctt cgtctctctg tcgaaaaaga agtaggtccc gcgggtccgg
      301 tcgctccccg cgtacaccag cactttcgtc tcgtcccggc tcatggaggc gacgacgacc
      361 tcgtcgtcag gaaggcgctc ttccagctcc ttctggagcg cggctcgctg ctcgtcgaag
      421 aagtggtagc gaaagcggtc cgttgtgaag cccgttccgg tgatcacctt tcgcttccgg
      481 gagcggaaga gcgaactcac gtcgacgtcc gggtgttcga agagcagctc ctcgtgctcg
      541 acagtctgcg ggtcgaagac gaagatcgcc gacttgtccc gcccgaggtt cgacgcgacg
      601 tagagttttt tgttgtcgaa cgtgaagaag agcggcgaca cgctttctcg gaagtccgtg
      661 gtcaggatgt tccggaaggg ctcgtcctcc ttgtcgcggt agagaagcga cgtgttcact
      721 ccgtcggagc gcaccgcgac ccgcaacttt ccatcgtggt ccgtcatcca ccccgcgata
      781 tcccccgggt tctcctccag cagcgtcaga tctcccgtcg ccaggttcag ccgatagacg
      841 tcgaagaggc gctggtccct tcggttcatg ccgatcagga gaaagtcgtc ctgctcctca
      901 aggctgtcga cgatggtcac gcgggtattc tcgaacggcg tcagctcgcg cgactcggtc
      961 ccgtcggacg agacggcgta gagccggtag ttctcatcgc cgcccctgtc ctgaaggtag
     1021 acgatcctgt cgttccccga ccagaagtag gccgggatat cccgctccgt cgcggaggtt
     1081 acgcgcacgg cttcctccgc gccgattttc tggacgaaga tgttcatccg ccgctcccac
     1141 ggcttcatga acgagatgta ctccccgttc ggagagagct ggaagccgct ctgctccgga
     1201 ttacggaaga aatcctccat agggatgaga ggctcgtccg ccctcgccgg gcgggggagc
     1261 agcagcgtca agagcgcgag cagcgcgatg gatgaaaaga gtttcatgat cttactcccc
     1321 catgaccttg acgaccacgc gcttgcggcg gcggccgtcg aactcggcgt agaaacagcg
     1381 ctcccagggc ccgaggtcga gcctgccctc cgttaccgga agaatcgcct gatggtggat
     1441 caagatacgc ttcagatggg cgtcgccgtt gtcctcccct gttctgtgat gaagatagga
     1501 cggatcttcc ggggctgtcc tttccagcca gctcatgata tcctcgtgaa ggccggattc
     1561 attgtcgttg acgataatac cggcggtgat gtgcatggcg gagacgagca tcatcccttc
     1621 gccgataccg ctccgcccca gaatgtcctg gagcgccggg gtgatgtgca cgagttcctt
     1681 gcgtttcttt gtctcgaacc agaggtattc cgtatggctt ttcatactga tctctccttc
     1741 gtgatggtgg ctgtacgtgg aatatggtag catactcttt tactgtcgac ccgttttgtg
     1801 ctctatcttg aaaaggaaga aattcgccgc tctccgaagg gacggcctga gcggaggatt
     1861 gcgaggtgat gccgaaatga ccgaatttag caatgatact cttttcgacg acggcgccgc
     1921 cgcaatgtgg cgtcaagacg cgggcatgct gagggccgtg ctcgacgtca tccccgatat
     1981 tgtttccgta cagagtccgc agcttgagat tctcttttac aacaaggcgg ggctcgattt
     2041 cctgcatgcg tcttccgaag gaacgatcgg ccggaactgt tatgcgctcc tcgggagaaa
     2101 agagccctgc gacgattgcc tctcgctcaa ggccatcaga acgggaaatg cccaggaggg
     2161 agagaaatac gttccggagc ttgacgtgtg gttcgaggtg cgcgccatcc cggtgaagga
     2221 cgacgcaggt agtgtccggt gcgttgtgga gcagctgcgc gactgcacgg caagaaaacg
     2281 gaacgaggag cgccttgcgc gcgttctcga agagcttgaa gcgaagaacg cggaacttgc
     2341 cgagacgagc gcaacggccc gtatgcttgc gctccgggcc gaggagtcca gccgggcgaa
     2401 gagcgctttt ctggcgaaca tgagccacga gatacgcacg cccatgaacg gaatcctcgg
     2461 gatggtcgaa ctcctgctgg atacggggct ctccatggaa cagcgggatt tcggagagac
     2521 gatcagcagc agcgccgatc atctgatgga gctgctcgac gacctgctgg acttctcgaa
     2581 gatagaagcg gagcggttga cgctggaaag cgccccgttc aatcttcagg aactcgtcga
     2641 ggatctgacg gtgcagactg cggtgaaagg acgggagaag aacctcgatg tctacgccgt
     2701 cgtcgaaccg gacgttcccg cattcttccg cggggacgcc tcgcgtcttc ggcaggtgct
     2761 cggcaacctg ctctccaacg ccgtgaaatt caccgatacc ggagaaatct cgcttgccgt
     2821 ctcccttgct caagacggta tcctcaggac gcttcttcgt ttcgaggttg cggataccgg
     2881 catcggcgtt ccggaggaga agaaggagct cctgttcaga ccgttttccc aggttgacgc
     2941 ctccatcacc agacgcttcg gcggaaccgg gctcggactt gccatttcga gggggctcgt
     3001 cgaactcatg ggaggaaaca tcggtatgaa ggacgggcag tccggcggcg ctcttttttg
     3061 gtttgaagtc cccctcgaca aaatcgacca cggcgacatc gaggatctcc ctgttgatcc
     3121 gaatctgatg aatacccccg tcctgcttgt cggaggaacg cccggatgga ggcgctccgt
     3181 aacgacgcac ctggagcgct ggctctgcgc cgttgcgtcg gcggaacgtg aaggggtcga
     3241 ggcgtgtctt gccggggctc tggacgcgga acggcctttt tccgtcgtgc ttgtcgacgg
     3301 gagcagcgtt ccggaagaca accccttttc ccctcgcgcg cttccggaga ggctccggag
     3361 cgtttcgtca ttctacctgc tcgactaccc ttcaaggcga tggaggaggg cggcgctcag
     3421 cgatgcaggg tacgccgatt ttgtgcccaa acctgtcaag agaaacgcct tgaagaacct
     3481 tctcctctcg ggcacagaaa gcgccggcag gatatactcc gagaatacgg gggttgcgcc
     3541 ggccggcaga aaggcgaaag tccttctcgt cgaggacagt gaagtcaacc ggaaggtggc
     3601 ctccgtcatg ctgcagcggc ttggatgttc ggtctcggcg gcgcatgacg gattcgaagc
     3661 gctggacttc ctgcgccgcg aggcattcga cctggtcttc atggatgtgc aaatgcccgt
     3721 tctcgacggg atcgacgcag tgaagcggat tcggagcggg tacggggaaa tcggacgccc
     3781 cgacattccg gtgattgcga tgaccgcgcg caccctgccc ggcgacagag aggagtgctt
     3841 cgcggcgggg atggacgact atatcgccaa gcctgtgaag atgaatgtgt tggaggaaat
     3901 tctcaggaag tggctgcctg gaggtgaagc acagtgaaca gcggagaacg acggatgcgt
     3961 attcgggagc agctcgcgct cgccggaggg ccggtcaagg gggcgatgct cgcttcgctc
     4021 ttttccgtga gccggcagat catcgtgcag gatatcgccg ttctgcgggc ggagggggcg
     4081 agcatcgttg ccactcccca ggggtacttg ctcctgccgg gaagggtgtc cggtatgctg
     4141 acaaagacag tggtgacacg gcacagcgac agctcgtcga tggaggagga gctgcgtatc
     4201 atggtcgacc ggggggcgag ggtgctcaat gtcatcgtcg agcaccctct ctacggggag
     4261 atcgccggga acctgatgat ctttacccgg agcgatgtgg accgcttcat ggaaaagatg
     4321 cgcgagagcg acggggaacc gctttcgacg ctcacgggag gcgtgcacct gcacacgctc
     4381 gaagtgcccg atgaaactac ctggagctcg gttctcgacg gcctgcgcga aaagaactac
     4441 ttgctggaag agaaataagg gtgtatgctt ttgtcaggtg taaagtcagc tgacaaaggt
     4501 ggtgcttgca atgaacgaaa cgctctcttc cgctgaagag gttggccgga agggcgcctt
     4561 ccgcggatac ctcgtcaagg tgtccaacgg tatggcgctc ggactcttcg cctcgctcat
     4621 catcggcctg atactccggc aggccggggg gctgctttca atgccctcgg tggagctatt
     4681 cggcaggata gcccagcttc tcatgggacc ggcgatcggc gccggcgttg cgtttgcggt
     4741 gggcgcgccg cctctaggac tcttctcctc gctgattgct ggagccgtgg gcgcgggaac
     4801 gttctccttc gagggcggcg ccgtggccgt cgctatcggc gagccggtgg gagcgctggt
     4861 cgcttcgctt gcaggagcgg agttttcgaa acggatcgcc gggaagacgg ggatggacat
     4921 catcgtcgtg cccgcgggga caattctcgt cggcgggttg gcgggggttg tagccggtcc
     4981 gttcattgcg gggatgatgt actggatagg gaacgcgatc aacgtcgcga caacgctgca
     5041 cccgatcccg atgggcatcc tcgtctcggt gatgatgggc gttgcgctta cgctgccgat
     5101 tagcagcgcg gcgctctcca tctctctcgg tctctcgggg ctggctgccg gggcctcgac
     5161 cacggggtgc tgctgccaga tgatcgggtt cgccgtggcg agctacaggg acaacggcat
     5221 tggcggtctc ttttcgcagg ggcttggcac ctcgatgatc caaattccca acatcatacg
     5281 gaacccgtgg atatggattc cgcccaccct tgcctccgct gtgctggggc cggtgtccac
     5341 ggtgttcttc aggatggaga atagccgggt cggcgccgga atgggaacca gcggcctcgt
     5401 ggggcaggtt tcgacgttcg agacgatggg cgttgccgca tggcccgggg tcctgctgct
     5461 gcactttctc ctgcccgccg cgctctctct gctcttcgcc gaactgttgc ggaagaccgg
     5521 gcgcatccgc cccggagata tgaagctgga gaagtaagag ctaccccaga tggagcacgc
     5581 gccgggcgac ctcgaagtag agcagcaggc cgaggatgtc gaccacagtg ctgatgaacg
     5641 gcccggacat gatcgcaggg tcgagcttga gtttttttgc ggcgaatggg aggagcgcgc
     5701 cgacgaggtt tccgagcatc acaacgccga cgaccgagat ggcgacggta agtgcaacgc
     5761 cgaa
//
LOCUS       DAOSWW010000043         5745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_9292_length_5745_cov_3.248330, whole genome shotgun sequence.
ACCESSION   DAOSWW010000043 DAOSWW010000000
VERSION     DAOSWW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9292_length_5745_cov_3.248330"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="PK442_01650"
     CDS             complement(<1..109)
                     /locus_tag="PK442_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOO86381.1"
                     /translation="MSLLRVKVPASSANLGSGFDTVGVALSLYNFFDVLE"
     gene            complement(106..1152)
                     /gene="thrC"
                     /locus_tag="PK442_01655"
     CDS             complement(106..1152)
                     /gene="thrC"
                     /locus_tag="PK442_01655"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOO86382.1"
                     /translation="MKKGILFSYGKYLPLTEKTPMLSLCEGDTPLVPLRNLSRELGVG
                     LFGKYDGLNPTGSFKDRGMVLAVSKALEEGAKAVICASTGNTSASAAAYAAVAGIPCF
                     VLLPAGKVALGKLAQAMIYGARVLAVRGNFDRALEMAREGAEQDGFAIVNSVNPYRLW
                     GQRSAAWEICDELGAPPDWLVLPVGNAGNISAYCAGFEFYKEQGITRNIPRLVGVQAE
                     GAAPMVNGAPVDAPETVATAIRIGHPVSADKAKRAVEISGGRFLAVSDDEILDAQKLL
                     ASSDGVFAEPASCAPLAGLLKLKREGGLPSGLRIVMVLTGNGLKDPDAATSRVPAPVE
                     IDGTREALMEVLHS"
     gene            1581..1913
                     /locus_tag="PK442_01660"
     CDS             1581..1913
                     /locus_tag="PK442_01660"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO86383.1"
                     /translation="MKPYDMAGIDPFTGRPGFIGKFVHSDQMTLAHWEIAGGTELTEH
                     SHPHEQITLLLAGKMELRSGDHVAILTPGQGIVFAGGETHGGYAHEDCVVVDIFCPVR
                     EDFKSQQK"
     gene            complement(2032..2709)
                     /locus_tag="PK442_01665"
     CDS             complement(2032..2709)
                     /locus_tag="PK442_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOO86384.1"
                     /translation="MRLNIFNCSPRGKRSNTKVLTNAFAEGFLRGAAGAPELNEVREF
                     FISEPDDFRRAAEAFGEPSAALIAFPVYVDAMPAIAKSFIEEIAAFKGRCSSTRMLFL
                     IQTGFPEETHTRPMKAYLEKLAKRLGAPCDGVIRMGGGEGARDRVRGSKGGGKMFERY
                     RMLGRGYAANGVLDEAILAKLAGPKRLPALLAPLVLPLANFFFWDRELKRNGVYEKRR
                     DRPLERE"
     gene            complement(2690..3238)
                     /locus_tag="PK442_01670"
     CDS             complement(2690..3238)
                     /locus_tag="PK442_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOO86385.1"
                     /translation="MRILFVDGNPSAEDAVFGERLAAMEHGVAGHDVTRFCLRGKAIH
                     SCIGCFACWMRTPGTCIFQDDHVELLRLFLKSDVAVFASPMIAGFYSALLKTAIDRVI
                     PLVLPYIEFADGECRHPLRYGRAMPKMGFLYEPEEDTDEEDLRIVSTAFERFARNGHT
                     RFLFSRPLSEDPEEVRHAIEHL"
     gene            3529..4851
                     /locus_tag="PK442_01675"
     CDS             3529..4851
                     /locus_tag="PK442_01675"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HOO86386.1"
                     /translation="MRKTFLLFVCLLLFNPFPAVAEQTFREFSGEFTPQNNGERLLAF
                     LVSVTDPESLELQMDALPGDDGAIRAVSFALHGGALGGFRIERLTLESAFIRLNPPSE
                     WIRGNRHSLRVRNALRSNFELVIDEHDILEALKTYASGSWSRISVDLTSGELRIQGRY
                     KPGATGFSLMAGITTRLELRDGKRVMLKAPEIRINGENKTSLFQRDLERLRPVLDFNG
                     FPPALTISRLSVDKGKLLLATATRPKAVGGIRYRYVRDLERPFSIHPPESFDFAERQF
                     RNGDIVLVGGKTWRSKIVNLFEDGNAAFSHSGIVRIIGGVPYIIHASPESELVRMERA
                     ETFLSPKKVDRASIYRLRGNPGAAETAGRKALEYYEERVPFDTAFDSEDHERLYCTEL
                     IWRAYTHAGIDLADGLWYSLYNPVLSGRLLLPNSLTRSPLLEETAILR"
     gene            complement(4908..5297)
                     /locus_tag="PK442_01680"
     CDS             complement(4908..5297)
                     /locus_tag="PK442_01680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOO86387.1"
                     /translation="MPGKVTSRFGQRTHPITKEPDYHMGVDIHVPHGAPIRAAKAGAV
                     VFSGLRGGYGNAVIIQHDAKSSSLYAHCSRLHVKRGRKVHQGQVIAAVGETGRATGPH
                     LHFEIRTGGGAVNPLEFWRKHAESSAQ"
BASE COUNT         1230 a         1836 c         1579 g         1100 t
ORIGIN      
        1 tttcaagcac atcgaagaag ttatacaggg agagggccac ccctactgtg tcgaacccgg
       61 agccgagatt cgcgctggag gcgggaacct tcacccgcag caggctcacg agtgcagcac
      121 ctccatgagc gcctcgcgcg tcccgtcgat ctcgacagga gcgggtacgc gggaggtcgc
      181 cgcgtcggga tccttcaggc cgttccccgt gagcaccatg acgatccgca gtccggaggg
      241 gagcccgccc tctcttttca atttcaggag tccggcgagc ggcgcacagg aagcgggctc
      301 tgcgaagacg ccgtccgagg aagcaaggag cttctgcgcg tcaaggatct cgtcgtccga
      361 aacggcgagg aaacgccccc cggagatctc gactgcgcgt ttcgccttgt ccgcgctcac
      421 cggatggccg atgcggatcg ctgtcgccac agtctcggga gcatcgaccg gagcgccgtt
      481 caccatcggg gcggcgcctt ccgcctgcac gccgacgaga cgggggatgt tccgcgtgat
      541 accctgctcc ttgtagaatt caaagcctgc gcagtaggcg ctgatgttgc ccgcgttccc
      601 tacgggaagg acaagccagt cggggggagc gcccagttcg tcgcagatct cccacgccgc
      661 gctcctctgc ccccacagac ggtacggatt tacggagttc acgatggcga acccgtcctg
      721 ctccgccccc tcccgcgcca tttcgagcgc cctgtcgaaa ttacccctga cggcgagcac
      781 tctggctccg tagatcatcg cctgcgccag cttccccagc gcaaccttgc ccgcgggaag
      841 gagcacgaag cagggaatgc ccgcgacggc cgcgtaggcc gcggcagagg ccgacgtatt
      901 gccggtggag gcgcagataa ccgccttcgc cccttcctcg agcgccttcg acactgcgag
      961 caccatcccc ctgtccttga acgaaccggt ggggttcagg ccgtcatact tcccgaacag
     1021 cccgacgccg agctcccggc tcaaattccg cagcggaacg agcggcgtgt cgccttcgca
     1081 cagcgacagc atcggcgtct tctccgtcag cggaagatac tttccgtaag aaaacagtat
     1141 gcccttcttc atggaacatc cctcctgtaa tacaaaaagc ccgcaccctc cacaatacag
     1201 gagacgatgc aggctcatcg cggttccact ccatttccgg acctctcgcg gctgcttcgc
     1261 gcatccgccg ggaggatacc ggcaactcga caccgctgta aggggcggac cccggcgtct
     1321 tccctccgcg tgcgaaaccg cgcgaacttc gacgccgact caggggtggt cttctctgcg
     1381 ctcctcggga gaagggcttc cagcctcggc ccttcctctc tgtccttcgg agagaaggta
     1441 ctcttccctt catcgtcttt ttatcatatc attggaaaac attttcctct tataccttcg
     1501 tcgccggaaa gtgtcaagag gggacccgga aaagaggggg cgcggaggac agatattcat
     1561 aaaggagcgt ggcatacgag atgaaaccct acgacatggc aggtatcgat ccgttcacag
     1621 gaaggcccgg attcatcggc aagttcgtcc attccgacca gatgacgctt gcgcactggg
     1681 agatcgcggg aggcaccgaa ctcaccgagc acagtcaccc ccacgagcag attacgctgc
     1741 tgctcgccgg aaagatggag cttcgctccg gcgaccacgt ggcgattctc acccccgggc
     1801 aggggatcgt cttcgcggga ggggaaacgc acggcggcta cgctcatgaa gactgcgtcg
     1861 tcgtggatat cttctgcccc gtgcgcgaag acttcaagag tcaacagaag taaacggcac
     1921 ggcgggagag agcgaacagc tcctcccgcc gtttcttccg cgtgcgcgcg atccggcgtc
     1981 attccatccc gccggagcat ttttccgtac aacgcacagt ttccgccgtt tttactcccg
     2041 ttccaacggt cgatcacggc gtttttcata cacgccgttc cgcttcagct ccctgtccca
     2101 gaaaaaaaag tttgccagcg gcaaaacgag cggcgccaac agcgcaggaa ggcgtttcgg
     2161 ccctgcgagt ttcgcaagga tcgcctcgtc gagaacgccg ttcgccgcat accctctgcc
     2221 gagcatccgg tagcgttcga acatttttcc gccgcccttc gatccgcgca cacggtcccg
     2281 cgccccctcg ccgccgccca tccggatgac tccgtcgcaa ggggcgccga ggcgtttcgc
     2341 cagcttttca aggtaggcct tcatcggccg cgtatgcgtt tcttccggga atcccgtctg
     2401 tatgaggaaa agcattctcg tcgacgagca gcgccccttg aaggcggcga tctcttcaat
     2461 gaaggatttc gcaatagcgg gcatcgcgtc cacatagacc ggaaaggcga tcagggcagc
     2521 cgacggctct ccgaaagctt ccgccgcccg ccggaagtca tccggttccg atatgaaaaa
     2581 ctccctgacc tcgttcagtt ccggcgcacc cgccgctccc cggagaaagc cctccgcaaa
     2641 ggcgtttgtc aacaccttgg tgttgctcct cttgccccgc ggcgagcagt taaagatgtt
     2701 caatcgcatg gcgcacctcc tcgggatcct cactcaaagg ccgggagaac aggaaacgcg
     2761 tgtgcccgtt gcgggcgaaa cgctcgaacg cggtggagac aatccggaga tcctcctcgt
     2821 ccgtatcctc ttccggttca tagagaaagc ccatcttcgg catggcacgg ccgtaccgca
     2881 gcgggtgacg gcactccccg tccgcaaact cgatgtacgg cagaacaagc ggaatgaccc
     2941 ggtcgatcgc cgtcttcagc aacgcggagt aaaaacccgc gatcatggga gaggcaaaga
     3001 cggcaacatc cgatttcaga aacaaacgca gcagctcgac gtgatcatcc tggaagatac
     3061 atgtccccgg ggtgcgcatc caacacgcga aacaaccgat gcaggagtga atcgcctttc
     3121 cccggagaca gaacctcgtg acatcgtgcc ccgcaacacc gtgctccatc gccgcaagac
     3181 gctcgccgaa aacggcgtct tccgcgcttg gattcccgtc gacaaacaag attctcaata
     3241 cgcaacacct cgccatcctt acaaatcccc ggaacgattg gctgaaaaat attccagagc
     3301 gccccgggct gaacgtccac ttccatcttt gattgcattc ttttctcagg tgcaaataaa
     3361 gcgtcattat aatacaggcg cttcgcctcg aatacagttc tctgatacaa tactacagaa
     3421 ttcttttatt ttgggcgata cacaccacac catgggcgtt gctaccgaga aaaatcacgg
     3481 atacgcgaag ccgtccgctt tttcactgca tcgaaagggg ggatcagcgt gaggaaaaca
     3541 ttccttctct tcgtatgtct tttgctcttc aatccgttcc cagccgtcgc ggagcagacc
     3601 tttcgcgagt tttccggcga gttcaccccc caaaacaacg gagagcggct gctcgcgttt
     3661 ctggtctcgg tcaccgaccc ggagtcgctg gagctgcaaa tggatgcgct tccgggagac
     3721 gacggcgcca tccgggccgt ttcttttgct cttcacggcg gcgctctcgg agggttccgc
     3781 atagaacggc tgaccctaga gtcggcattt atacggctca atccgccctc tgagtggatt
     3841 cgcggcaacc ggcattcgct ccgggtacgc aacgcgctcc ggagcaactt cgaacttgtg
     3901 atcgacgaac atgatatcct cgaagccctg aagacatacg cctcggggag ctggagccgc
     3961 atctccgtgg acctgacgtc gggcgagctt cgcatacagg ggcgctacaa gcccggtgcg
     4021 acaggcttct ccctcatggc cggaataacg acgaggctcg aactgcgcga cggaaagcgc
     4081 gtcatgctga aagctccgga gatacgcatc aacggcgaga acaagacatc tcttttccaa
     4141 agagatctcg aacggctccg ccccgttctc gacttcaacg gttttcctcc ggcgctgacg
     4201 atctcacgcc tctccgtcga caaagggaag cttcttctgg cgaccgcgac gcgtccgaag
     4261 gcggtcggcg gcatacgcta ccgctatgtc cgcgacctgg aaagaccttt ctccattcac
     4321 ccgccggagt cgttcgactt cgccgagcgg cagttccgga acggagacat cgtactcgtc
     4381 ggcgggaaaa cgtggcggag caagatcgtc aatctttttg aagacggaaa cgccgccttt
     4441 tcacatagcg gcatcgtcag aataatcggc ggcgttccgt acatcatcca tgcatctccc
     4501 gagtccgagc tggtgcgaat ggaacgcgcg gagacttttt tatccccgaa aaaagtggac
     4561 agggcaagta tctaccgtct gcgggggaat ccgggcgccg ctgaaacagc cggcaggaaa
     4621 gcccttgagt actacgaaga gcgcgtgccg ttcgacaccg cgttcgacag cgaagaccac
     4681 gaaaggcttt actgcaccga gcttatctgg agagcgtaca cgcacgcggg tatcgacctg
     4741 gccgacggac tgtggtactc cctgtacaac cctgtgctct ccggcagact gctgcttccg
     4801 aacagcctga ctcgaagccc gctgctcgaa gaaacggcga tcctgcggta aaccgggagg
     4861 gcggcgcggc gcttcgatgt ccgatgtgaa cggccgcact gcggttttca ctgcgccgac
     4921 gattccgcgt gcttcctcca gaactccaaa ggattgaccg cgccgccgcc cgtgcgtatc
     4981 tcgaaatgaa ggtgcggccc cgtcgctctt cccgtctcgc cgaccgccgc gatcacctgt
     5041 ccttgatgca ccttccgccc tcgcttgacg tgaagacggg agcagtgagc atataaggag
     5101 gaacttttcg catcatgctg tatgatgacc gcgttgccgt accctccgcg cagaccggag
     5161 aaaaccacgg cgcccgcctt tgccgcccga ataggggctc cgtggggaac atgaatatca
     5221 acccccatgt ggtagtccgg ctccttggta atcgggtgcg tcctctggcc gaagcggctt
     5281 gtcacctttc ccggcacgaa caagggccat aaaaacccca gcttcgcaat cccctgggaa
     5341 atggagaccc cggaggagtg cggcgcattt tcctgcggtt gagaaaacac ggtaaaacaa
     5401 ggtacgcaca ggcagcccaa caccgctgcg atcattatga gtcgatacaa ccaaacgttt
     5461 tcaccccgat acatcacaaa acaccctctc gattctcttg agacgcgccg ccccaaaaaa
     5521 gtggcggatc tctttatcat attgtacagt cttttcccga aaatcccatc gtccacgcgc
     5581 ctcaaagccg cagagcaaac aagggggctt cctgtcaaag agacaaacga acggcagaca
     5641 ctttgtattt tgccattcat ccttcggtca agagaagaag aggatatgta agagaagcat
     5701 attgataggc aaaacaatgc tcaagtgaga taatgaggat gcata
//
LOCUS       DAOSWW010000044         5712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_9364_length_5712_cov_3.957575, whole genome shotgun sequence.
ACCESSION   DAOSWW010000044 DAOSWW010000000
VERSION     DAOSWW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9364_length_5712_cov_3.957575"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PK442_01685"
     CDS             complement(<1..609)
                     /locus_tag="PK442_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO86388.1"
                     /translation="MEWFWPEGFYTIVMVGSFVLGAFALKLPIAIALSGAAVVGALAG
                     GEWFPLRHFVEGMFGYLDTILIIASAMIFMKSVQKTGLLESLAAWVIRRFRRKPLLLS
                     VGLIFIIMAPGMITGSSTAAVLTTGALVAPVLIKLGVPAVKTAAAIAMGAIYGMIAPP
                     INIPAMIIGGGIDMPYVGFALPLLVCTIPLAIFSALLLVYPSL"
     gene            complement(625..1200)
                     /locus_tag="PK442_01690"
     CDS             complement(625..1200)
                     /locus_tag="PK442_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOO86389.1"
                     /translation="MKRIILCVVAAALFLSCGLTLPAMVSSAAASEIVLTSVGQSPDA
                     TMIRVVLRKMKINAENQPLLRPEGLAGEKILVAVVGGSSKGLGAAGIDKDQEIERVTG
                     LFQSAKQKGMKLLIMHIGGEGRRGTLSDAFIEAAAPHADRLIVVDGGNNDKIFDHLVE
                     GKNVKILTAPNVNGTAEPLKATLSEWGVSAQ"
     gene            1378..3492
                     /locus_tag="PK442_01695"
     CDS             1378..3492
                     /locus_tag="PK442_01695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO86390.1"
                     /translation="MPANLRSIRGVLNFYTLLLAVAMIIFPVLIGVYDLYKASRTVER
                     DIAYALDMQSVALQRWFAIHQNNIASIASLPSVQTKAYDDMFEDFQSFVRTNTNFDSI
                     VYVNTEGRGVVNSTTGFNPRPETDVSDRDYYKDAVKGKPHISDILIGRTSGMPVVIFS
                     APLVGDDGFQGLVFGAIRFDTLLNTILSTHFGATGQFLLLNSKGRSLQGTGESSFPNE
                     TLAALLSSDGYSVSYRDASGKRYLAKGKKLEKTNFFLVAQMDYGEFIAPFIGNMVYFV
                     TVSAVLLFFMLSISRKLYKKVDRSLNLLFEGVIKTEQGEYDSLDPELLEEAPFELREL
                     GIAFSSMAETVRSKTAELEFRSFHDELTGLYNRAYFEDAMRRFDSGRFNPVTVVVCDV
                     NGLKMINDTLGHKAGDELIVAAAHSLANAGRKTDIVARIGGDEFAMLHPESTEETAGE
                     VLRRIRAEIDKYKRGEDSLPLNIACGAATTSGRIQNIEALLSEADKQMYASKQLEKPH
                     SKREILEYLSSRIHDLRKASHDRKRHMAACEAIMEAFVREQPDIAPEKKELLILLAKN
                     HDIGFTEIPSEITEKPGPLTDEEYLLVQRHPETGARIASLFPELADLAEPIRLHHRWW
                     NGKGYPAGDGGEAIPLESRLMAIVDAYEAMTGDKIYRTPLSPEEALKELHRCAGSQFD
                     PVWTERFITFFREYREREHIFA"
     gene            3640..4686
                     /gene="rlmN"
                     /locus_tag="PK442_01700"
     CDS             3640..4686
                     /gene="rlmN"
                     /locus_tag="PK442_01700"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOO86391.1"
                     /translation="MNESVNALDLSYEEWIETSVTRWKLPRYRADQLCQWIYDKKVFN
                     VHEMTNLSKDLREKLVYELMILPPIAVREDTSKDGTKKFLWQLYDGERIESVLLSHGS
                     HMTACISSQVGCPLNCSFCATGKSGFTRNLSAGEIVGQFLAMEKRIGENINNVVYMGM
                     GEPFLNSAEVFKSIRILNSSKMRNLGARHITISTSGVVPGILELADFPIPVRLSVSLH
                     APNDVLRSKIMPVNRKHSLGSLIDALKAYKDKTGERITIEYVMLQKVNDEPEHAYEMA
                     AILSGLGVYVNLIPYNPIALLQPGEQGFQRSTTERIKEFAAILAKLGIENEIRKEKGT
                     DIDAACGQLRAQSM"
     gene            4733..5062
                     /locus_tag="PK442_01705"
     CDS             4733..5062
                     /locus_tag="PK442_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOO86392.1"
                     /translation="MKRPQRKDGNTTLSLRDLPNGSLARIVKILSPLHNAAETGSSCC
                     AARLEALGLRVGKIVEKVSGMPFHGPVTLLLDGRQIAVGWNISSHVLVVPVKERERAH
                     GRSAERE"
     gene            5037..5696
                     /locus_tag="PK442_01710"
                     /pseudo
     CDS             5037..5696
                     /locus_tag="PK442_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807469.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
BASE COUNT         1360 a         1618 c         1575 g         1159 t
ORIGIN      
        1 cagggagggg tagacgagga gcagcgccga aaagattgcc aggggaattg tgcagacgag
       61 aagaggcagg gcaaagccga cgtacggcat gtcgatgcct ccgccgatga tcatcgccgg
      121 gatgttgata ggcggtgcga tcataccgta gatagcgccc attgcgatcg ccgcggcagt
      181 cttgacggcc ggaacgccga gttttatcag aacgggagcg acaagggctc ccgtggtgag
      241 aacggccgcg gtcgacgaac cggtgatcat accgggcgcc atgatgatga agatcaaccc
      301 gacgctgagg agaagcggct tgcggcggaa tctgcggatt acccacgccg cgaggctctc
      361 cagcaacccc gtcttctgca ctgatttcat gaagatcatc gcggaggcga tgataagaat
      421 cgtatccaga taaccgaaca taccctccac gaaatggcgg agagggaacc attcgccgcc
      481 ggcgagagcg ccgacgaccg ccgcgccgga gagtgcgatc gctatgggga gcttcagcgc
      541 aaacgcgccg agaacaaagc tccccaccat cacgatcgta tagaatcctt caggccagaa
      601 ccactccatg acagggcctc ccgcctattg ggcggatacc ccccactccg agagggttgc
      661 cttgagaggc tccgccgtgc cgttcacatt gggcgcggtg agaattttga cattctttcc
      721 ctcgaccaaa tgatcgaaaa tcttgtcgtt gtttccgccg tcgaccacaa taagcctgtc
      781 ggcgtgagga gcggcagctt cgataaaagc gtcggagagc gtccctcttc tcccttcgcc
      841 gccgatgtgc attatgagga gcttcattcc tttctgcttc gccgactgga agagtcccgt
      901 cactcgctca atctcctggt ccttgtcgat gcccgccgcg ccgagcccct tggagcttcc
      961 tccgacaacg gcgacgagaa ttttctcgcc ggcaaggcct tcaggtctga gcaggggctg
     1021 gttttccgcg ttgatcttca tcttccgaag caccacgcga atcattgtcg catcaggact
     1081 ctgacctaca gaagtgagca cgatctccga agccgccgcc gaagagacca tagccggaag
     1141 cgtaagacca caggaaagga acagagcagc cgccactacg catagaatta ttcttttcat
     1201 ggcacatcct cctcaaaaat agaatatacg attcatacgt tctttttaag tgtacccttc
     1261 aggagttttc tgtccatatt tcttatcatt cttttcaata gaaacatcga aggctgcggt
     1321 atacagcacc ctatggtatt atttgaatag aaaaaagaag acgggaggta catgcaagtg
     1381 cccgctaatt tacgaagtat tcgcggcgtg ctcaactttt atacgcttct ccttgcagtt
     1441 gcaatgatca tctttccggt gctcatagga gtgtacgatc tctacaaggc ctcgaggact
     1501 gttgagcggg atatcgcgta cgcgctggat atgcagtctg tggcgctcca gcgatggttc
     1561 gccatacacc agaataacat cgcctccatc gcaagccttc cgtccgtcca gacaaaagca
     1621 tacgacgata tgttcgaaga ttttcaaagt tttgttcgta caaatacgaa ctttgattcc
     1681 atcgtctatg tcaacacgga aggaaggggc gttgtcaaca gcactacagg cttcaacccc
     1741 cgccccgaaa ccgatgtttc cgaccgcgac tattataagg acgccgtcaa agggaagcct
     1801 catatctccg acattctcat cggccggaca tcgggcatgc ccgtcgttat tttctcggct
     1861 ccgcttgtgg gagacgacgg cttccaggga cttgttttcg gcgccatacg tttcgacacc
     1921 cttttgaata ccatactgtc aacacacttc ggagcgacag gtcagtttct cctcctgaat
     1981 tccaagggca gatctttgca gggaacggga gaaagcagtt ttcccaacga gacgctcgcg
     2041 gctctcctct catccgacgg atattcggtg agctaccgcg acgcttcggg caaaagatat
     2101 ctcgccaagg gcaagaaact ggaaaaaacg aactttttcc ttgtagctca aatggattac
     2161 ggtgaattca ttgctccatt catcggcaac atggtctatt tcgttacggt ttcagcagtc
     2221 ctcctttttt tcatgctctc tatctctcgc aagctgtaca agaaggtcga cagatcgctc
     2281 aacctgctct tcgaaggggt aataaaaacc gaacagggag agtatgactc tctcgaccct
     2341 gagctgctgg aggaggcccc ctttgaactg cgggagctcg gaatcgcctt cagctcgatg
     2401 gcggagacag tgcgaagcaa gactgctgag ctggagtttc gctccttcca cgatgaattg
     2461 accggcctgt ataaccgcgc gtatttcgaa gacgctatgc gccgcttcga ctccggccgg
     2521 ttcaatcctg taaccgttgt ggtctgcgat gtgaacggcc tcaaaatgat caacgatact
     2581 ctcggacaca aggccggcga cgagcttatc gtcgcagcgg cgcactcgtt ggcgaatgcc
     2641 ggccggaaaa cggatatcgt cgccaggatt ggaggcgacg aattcgcgat gctccatccc
     2701 gaatctactg aagaaacagc aggagaggta ctccggcgta ttcgggccga gatcgacaag
     2761 tataagcgcg gcgaagattc gcttccgctg aacatagcgt gcggggcggc aaccacctca
     2821 ggccggatac agaatatcga ggctctttta agtgaggccg acaagcagat gtacgcatcc
     2881 aaacagcttg aaaaaccgca ctccaaacga gaaattctgg agtatctctc ttccagaata
     2941 cacgacctcc ggaaggcgtc tcacgacaga aagagacaca tggcggcgtg cgaggcgatc
     3001 atggaagcgt tcgtccgaga gcagccggac atagctcccg agaaaaaaga gctcctcatt
     3061 ctcctcgcaa aaaaccacga catcgggttt accgagatcc cgtcggagat taccgaaaaa
     3121 ccgggaccgt tgacggacga agaatacctg ctcgtccagc gccacccgga gacaggggcg
     3181 cgtatcgctt cgctctttcc ggaacttgcc gacctggcgg agcctatccg tctgcaccac
     3241 cgatggtgga acgggaaagg gtaccccgca ggagacggag gagaggcgat cccgctcgaa
     3301 agccgcctca tggcgatcgt cgacgcctat gaagccatga cgggagacaa gatctacaga
     3361 acgccgctct ccccggaaga agcgctgaaa gagcttcacc ggtgcgccgg gagccagttc
     3421 gacccagtat ggaccgaacg ttttattacg ttttttcgcg agtatcggga gagagaacat
     3481 atctttgcat gaacggccgg catccggaga gattccgtac cctgcatggg ttttcctaga
     3541 gaattcggcg gaacaggcat tgacagagag catctttcca tgacatgatt cagtgttaga
     3601 attgaaacgg cgttcttgaa cgcctcagga ggcagcggta tgaacgaaag cgtcaacgcc
     3661 ctcgatctct cctacgaaga gtggatcgag acaagcgtca caaggtggaa gcttccacgc
     3721 tacagagccg accagctctg ccagtggatc tacgacaaaa aagtcttcaa tgttcacgag
     3781 atgaccaacc tctcgaagga tctgcgggag aagctcgttt acgaactgat gatccttccc
     3841 ccgatcgccg tccgcgagga tacgtcgaag gacggcacga aaaaattcct ctggcagctt
     3901 tacgacggag agcggatcga atccgtcctg ctgtcgcacg gctcgcatat gacagcctgc
     3961 atctccagcc aggtggggtg tcctctgaac tgctccttct gcgccacagg aaaatcgggt
     4021 ttcacgcgca acctcagcgc cggcgagatc gtcgggcagt tcctggcgat ggaaaaacgc
     4081 attggagaaa acatcaataa cgtggtctat atggggatgg gcgaaccttt cctgaattcc
     4141 gccgaagttt tcaagtcgat ccggattctg aattcgtcaa agatgcgcaa cctgggggcg
     4201 cgccacatca caatctcgac atccggcgtc gttccaggca ttttggaact ggcggacttc
     4261 ccgattccgg tgcgtctttc cgtctccctc cacgcgccga acgacgtgct gcggagcaaa
     4321 ataatgccgg tgaacaggaa gcactctctg ggctcgctca tcgacgcgct gaaggcctac
     4381 aaagacaaga caggggaacg gatcacgatc gaatacgtca tgctgcaaaa agtcaacgac
     4441 gagcctgagc acgcctacga aatggccgcc attctttcgg ggctcggcgt ctacgtgaac
     4501 ctgataccgt acaatccgat cgcgctcctg cagccggggg agcagggatt tcagcgttcg
     4561 acgacggagc gcatcaagga gttcgcagcc attcttgcca aactcggcat cgaaaacgag
     4621 atccgcaagg aaaaaggaac ggacatcgac gctgcctgcg gccaactgcg ggcgcagagc
     4681 atgtaagggg gtcgtcagaa gagtgaacct cctccaacgt gtcgcccgct ccttgaaacg
     4741 cccgcaaagg aaggacggca acacgacgct ttcgttgcgc gacctcccga acggatcttt
     4801 ggcccggatt gtcaagatcc tgtctccgct gcacaacgca gcggagacag gatcttcatg
     4861 ctgtgccgca cggcttgaag ctcttgggct tcgcgtcggc aagatcgttg aaaaagtgtc
     4921 cgggatgccc tttcacggac cggtaacact gcttctcgac gggcgacaga tagccgtcgg
     4981 atggaatatt tcgtcacacg tactggttgt tcccgtgaaa gaaagagagc gcgctcatgg
     5041 acgaagtgcg gaacgagagt aaaaagcttc tgctcgtcgg caacccgaac gtcggcaaga
     5101 gcgccctctt cacccgaatc acaggcgttc acgccgtttc gtcaaactac cccggcacga
     5161 ccgttggatt cctggaaggc tggcttcgcc ggagcggcga tgtgtggcgt gtaatcgacg
     5221 ttcccggagc gtacacgctc tcgcctacga acgaagccga ggaagtagcg gaacgaatgg
     5281 tcaacgaggg agcggatgtt gccgtgcttg ttctcgacgc gacggccctg gaacgcaacc
     5341 tctttctcgc cttccagacg atggagcggc gcctgccggt tatcatcgcg ctcaatatgg
     5401 tcgacgaagc ccgccacaag ggaattgacg tcgatcccga ggcgttggaa agactgctcg
     5461 gcgttccagt cgtccctacg gtcgcagtga ccggacaggg aatcagccgt ctgctggacc
     5521 gtctcccgga agcccgtccc gccaggatcg cgctccccgg agggtacgct tccgaaaccg
     5581 gcgttcccgg cagggaagag cgctggagcg ccatcggtga gataatcgaa caggtccaga
     5641 gagtcagtca caggcaccac acgctcctcg acaagcttga ggacgcttcc gtggactctt
     5701 tttagggact ga
//
LOCUS       DAOSWW010000045         5691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_9415_length_5691_cov_4.738112, whole genome shotgun sequence.
ACCESSION   DAOSWW010000045 DAOSWW010000000
VERSION     DAOSWW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9415_length_5691_cov_4.738112"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1575)
                     /locus_tag="PK442_01715"
     CDS             complement(256..1575)
                     /locus_tag="PK442_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO86393.1"
                     /translation="MHEKSALIREDERKSMEKRALAYRWVVWGVMVFAFMVVFFHRLA
                     AGVVKEDVTSAFSLSATAFGAMSSMYFYAYMAMQIPVGLLADSLGARITVSVGMFAAG
                     AGSILFGFAPSYFWLLAGRFLVGLGVATVFVCIMKIQSQWFRDREFATISGATTFIGN
                     AGGIFSQGPLAFLLTIVSWRSSFIAIGIATLGISLLCYRLIRNRPQDMGFPPLNEREM
                     LREEMEAEPFSVLKGLKTVLSVRGVRIATLFYFFNQAGLFAFLGTWGTPWLVHVFEMS
                     IATASSLMVMTVIGIMIGGISTGWISDKIGRRRLPMIIAASVHSVLWGCMIFPWQQPL
                     GVGALRCIFLLLGFTNTAFVLSWSVAKELTSKKYTGLAISVLNAAGFFAIAVTTSIMG
                     MVIDYYSFLPLEAAYRRAFSLGLICSVFSLLFALLIPETGFMKRKEK"
     gene            1749..3707
                     /locus_tag="PK442_01720"
     CDS             1749..3707
                     /locus_tag="PK442_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO86394.1"
                     /translation="MIHLINITLRFGERVLFKNISWSIPKGSRVGLVGSNGAGKTTLL
                     RILSGEMESDSGEVSLAKDQSIGYLPQDLIEIPDVPVLDFLRERTGLADIERRLASCT
                     RNLTEAVPGSEEGNRLLTLHDELMKQYEMRDGFAFEPRARKILKGLGFTDEDAFSTCG
                     NFSGGWKMRLHLASLLLISPDILLLDEPTNHLDTESMEWLEGWLSVFPGTIIAVSHDR
                     RFLDTMCTSVAELSLGSLSLYQGNFSAYVEERERRIDDLRKAQKQQKEEIAHIEEFIE
                     RFRYKASKASSVQSRIKRLEKIELVEIEEETKRVHFRFPPCTRSGLESIVLKDVEKRY
                     GDKIVFHDASLSIQRGEKIALVGVNGAGKSTLSRILGGTEQPSSGSVRFGHNVKLAFF
                     SQESSQNLDYSRTVWDTISSRNAAWSEREKRSLLGAFLFGGDDIYKPVSVLSGGEKSR
                     LALLKLLLEEANVLILDEPTNHLDMQTKDLFQRALLEYDGTLVIVSHDRFFLDNLVDK
                     VIEITSGSLIVYPGNYSWFIEKRRERLASEASLLSFGNANASSEKSSQDGIRNLKRME
                     AEKRNALYRRKKRVLDTMEPLEARIEHLERDQAQRDAQLCDPSFLADSVKVSAILIER
                     NEAAREIADLLAQWEELAEEVDRIERTG"
     gene            3959..4594
                     /locus_tag="PK442_01725"
     CDS             3959..4594
                     /locus_tag="PK442_01725"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HOO86395.1"
                     /translation="MILENEPNDEGLIRELYLDVTGCDIGGVRIDSLKLRAVGVTLSP
                     PSQWEQEGGLDAKEVLNVHAFASITEKDLNENLLQKQFGDDDEWHNLQVRMTPDGIYA
                     RGNYHAQVLFTLNILIEIFSKFKIVDMQQVWLDDYTLRVNRVDVPDFITEKAVSQIQP
                     LLDLSKFVFPLRLSSITFKEGSMTIASRLLPEPFEGIVYTYEKNKKTEEKK"
     gene            complement(4647..5543)
                     /locus_tag="PK442_01730"
     CDS             complement(4647..5543)
                     /locus_tag="PK442_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO86396.1"
                     /translation="MDFHLVDLPLSRPGFRRFVSAWLLRDTARDRTYIVDPGPPSTWP
                     LLRDAVHSKGNGRVDAVLVTHVHIDHAGASGLVVREFGALVAAPEKGVPHLLDPSALW
                     KGSRQTLGETALLYGEPPPVPAEKILSIDSLPKGFFAVDTPGHAAHHQSFLFDDDGVL
                     TLFAGEAAGVYLEGEEPFPYLRPATPPRFFPDVTLASLDRLLERGCSRICYGHYGAAE
                     GAREMLTFAKEQLLFWKDVILGLLRNTADPRNEEFFLEELLERDPFLAAWRRMDHDIR
                     ERERMFLCNSIRGYLGAFSREK"
     gene            complement(5553..5684)
                     /locus_tag="PK442_01735"
     CDS             complement(5553..5684)
                     /locus_tag="PK442_01735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOO86397.1"
                     /translation="MHGTHDPDSIGRMITQGCVRLRNEDLEELKDKVFIGMKVIISE"
BASE COUNT         1516 a         1487 c         1367 g         1321 t
ORIGIN      
        1 atttcacagt tccctgactc atccaaagac agcctcctaa aactttatta aattctgcgg
       61 tccatatcct gatgccggac tgcaaatcaa caatatagga ttttaggcct tatgtcaagc
      121 tttttttaaa acttttcctt tttacgttcg tatcgattcc ttttttaccc ccctcgagtc
      181 tttggcatga ttaaaatgaa aaggcttttc gtgagatgaa attaaactaa atttcatata
      241 gagtccaccg cgcgtttatt tctcttttct tttcataaac cctgtttctg gaataagaag
      301 agcgaaaaga agagagaaaa ccgaacaaat caaccccaac gagaacgccc ttcgatatgc
      361 ggcttccagg gggaggaacg aatagtagtc gatcaccatt cccataatgg atgtcgtcac
      421 tgcaatggcg aaaaaacccg ccgcattgag gactgaaatc gccaatccgg tgtacttttt
      481 tgaagtcaac tcttttgcca cggaccagga aagcacgaat gcagtattgg tgaatccgag
      541 gaggagaaaa atacaccgca gcgccccgac accgagcggt tgttgccatg gaaagatcat
      601 gcacccccaa agcactgaat gcactgatgc ggcgataatc atggggagcc gtcttctccc
      661 gatcttgtcc gagatccatc cggtagagat accgcctatc atgataccga tgactgtcat
      721 caccataagg gatgaagccg tcgctatcga catttcaaag acatgcacaa gccatggagt
      781 tccccacgtc ccgagaaaag cgaacaaacc tgcctggttg aagaagtaaa agagcgtcgc
      841 gatacgtacc cccctcacag agagcactgt tttcaatccc ttgagaacgg agaacggctc
      901 tgcttccatt tcttcacgga gcatctcgcg ttcgttcaac ggcgggaagc ccatatcctg
      961 cggacgattt cgaatcaacc tgtaacatag aagagaaata cccaaagtcg ctattccgat
     1021 cgctataaaa cttgatcgcc aggatactat tgtgagaagg aaagcaaggg gaccttgcga
     1081 aaagattccg ccggcgttac cgataaacgt tgttgcaccc gagattgtgg cgaattctct
     1141 atcccggaac cactgggact gtattttcat tatgcataca aaaactgtcg cgacgccaag
     1201 ccctaccaag aaacggccgg caagaagcca aaaatacgac ggagcgaagc cgaaaagtat
     1261 cgatcccgct ccggcggcga acatccccac cgaaacggtt atcctggctc ccagggagtc
     1321 cgcgagaagt ccgaccggta tttgcatggc catatatgcg taaaagtaca tcgaagacat
     1381 ggcgccgaac gctgtcgcgc ttaatgaaaa cgcactcgtt acatcttcct tgacgactcc
     1441 ggcggctaac cgatgaaaaa aaactaccat aaatgcaaaa accattactc cccatactac
     1501 ccatcggtag gcaagcgccc gcttctccat agattttcgc tcatcctcac ggatgagcgc
     1561 actcttttcg tgcatgtgaa caattccttt cttcacgccc cttcgcaggt cggcgtattc
     1621 tacgcattca ttttacaatg ctgtagaaga taatgatagc atcatacaat ttttccaaac
     1681 ctcttttatc aatacgccgt accccttata atgaaccctg tacacgagga acaaggagga
     1741 acttttcggt gatccatcta ataaatatca cgctccgttt cggagaacgc gtacttttta
     1801 aaaatatttc ctggtctata cccaaaggtt ccagagtcgg ccttgtggga agcaacggag
     1861 ccggaaaaac cacactgctc cgcatactct ccggagagat ggaatccgac tcgggtgaag
     1921 tatctctagc gaaagatcag agcattggct atcttccgca ggacctcatc gaaattccgg
     1981 atgttcctgt tcttgacttt cttcgcgaga gaaccggcct cgccgacatc gaaagaaggc
     2041 ttgcgtcgtg tacgcggaat cttacggaag ctgttccagg atccgaagag ggaaacaggc
     2101 ttctgacgct ccacgacgaa ctcatgaagc agtatgaaat gcgagacggt ttcgctttcg
     2161 aaccaagggc tcgaaaaatc ctcaaaggac tcggttttac cgatgaggac gctttttcta
     2221 cctgcggaaa tttctccgga ggatggaaga tgcgccttca cttggcgtct ctgcttctca
     2281 tctctcccga catactcctt cttgacgagc cgacgaacca ccttgatacc gagagcatgg
     2341 agtggctgga agggtggctt tcagtgtttc cggggacgat tatcgccgta tctcacgata
     2401 ggcgtttctt agatacgatg tgtacttcgg tcgcggagct ttcactggga tccctttcac
     2461 tgtatcaggg gaatttttcc gcgtacgtgg aagagaggga acgtcgcatc gacgatctcc
     2521 gcaaggccca gaaacaacaa aaagaagaga ttgcacacat cgaggaattc attgagcgat
     2581 tccgttataa ggcctccaag gcgtcttcgg tacagagcag gatcaagcgt cttgagaaga
     2641 ttgagcttgt cgaaatagaa gaagagacaa agcgtgtcca tttccgcttt ccgccgtgta
     2701 cgcgaagcgg acttgaatcg attgtcctca aggatgtcga aaaacgatat ggcgacaaga
     2761 tcgtttttca tgatgcttct ctctccattc aaagagggga gaaaattgct ctggtcgggg
     2821 tgaacggcgc agggaagtcg acgctctcac ggatcctggg cgggacggaa caaccttcct
     2881 ctggctcggt tcgtttcggc cataacgtca aactcgcatt cttctcgcaa gagtcctctc
     2941 aaaatctgga ctactcaagg actgtctggg atactatctc ttctcggaac gcagcgtggt
     3001 cggaacgtga aaagaggagc cttctcgggg cgttcctctt cggaggcgac gatatttata
     3061 agcccgtttc tgttttgtct ggaggggaaa aatccagact tgctctcctg aagcttcttc
     3121 tggaggaggc gaacgtactg atcctcgacg agcccacgaa ccatctggat atgcagacga
     3181 aagacctctt ccagagggcg cttctggagt acgacggcac gttggtcatt gtttcgcacg
     3241 accgtttctt cctggataat ttagtagaca aagtaatcga aatcacttcc ggttctttga
     3301 tcgtctaccc cgggaactac tcctggttca tcgaaaaaag aagggaacgt cttgcttcgg
     3361 aagcttctct tctttccttt ggaaacgcaa acgcatcatc cgagaaatcg tctcaggacg
     3421 gaatacgaaa ccttaaacgg atggaggctg agaaacgaaa cgcgctctac aggcgcaaaa
     3481 aaagggttct ggacacaatg gaacctcttg aagcgcgtat cgaacacctt gaaagggacc
     3541 aggcccaacg cgatgctcag ctctgcgatc cttcgttcct tgcagattca gtcaaggttt
     3601 cagcgattct tatcgaacga aatgaagccg cccgcgagat agcagacctt ctcgctcaat
     3661 gggaggaatt ggcggaagag gtggatcgta tcgagagaac ggggtaaaac gccgatatac
     3721 caagagacct tcaaaaaatg cactgcatat ctcgtgagga ggacacatga tgcgtatttt
     3781 tttaattccg tacagtcgcg gcaataccag agaaagggcc tttctccttc tctttgtttt
     3841 tagccttgca ctgttcatgc tatcgcaggc aaacacagct tccgctgaaa cggatcaaac
     3901 gcggggggaa aaactgctcc tgcatttcgt agaggagttc aaacctgaaa aaatacacat
     3961 gatcctggaa aatgaaccta acgacgaagg ccttatccgg gaattgtatc tcgatgtcac
     4021 gggctgcgac atcggcgggg tccgtataga ttctctgaaa ctacgcgcag tcggcgtaac
     4081 gctctcccct ccttctcaat gggaacaaga gggcgggctc gacgcaaaag aagtactcaa
     4141 tgtccatgcg ttcgcctcca ttacggagaa agacttgaac gaaaatttgc ttcaaaaaca
     4201 gttcggcgac gatgacgaat ggcataacct tcaggttcgg atgaccccgg acgggatata
     4261 cgcgcgcggt aattaccacg cccaggtact gtttacgctc aatatcctca tcgagatatt
     4321 cagtaagttc aagatcgtcg acatgcaaca ggtgtggctt gacgactata cgctccgggt
     4381 gaaccgtgtc gacgttccgg acttcatcac ggaaaaagca gtctctcaaa ttcaaccgct
     4441 cctcgacctg agtaagttcg tctttccttt gcgcctctct tccattacct tcaaggaggg
     4501 gagcatgacg atcgccagcc gtcttctacc ggaacctttc gaaggaatcg tctacaccta
     4561 cgagaaaaac aaaaagactg aggagaaaaa gtaaacgcac gctgaataca aagcggggag
     4621 gttccgaacg gagccctccc cgttatttat ttctcccgtg aaaacgcccc aaggtaccct
     4681 cttatgctat tgcataaaaa catacgctcc cgctctcgta tatcgtgatc catacgccgc
     4741 caggcggcaa ggaaagggtc ccgttccagg agttcttcca ggaaaaactc ttcgtttctc
     4801 ggatccgctg tgtttcgaag gagcccgagg ataacgtcct tccagaacaa cagctgctct
     4861 ttggcgaatg tgagcatctc tctcgcacct tccgccgcgc cgtaatgtcc gtagcagatt
     4921 cgggagcatc ctcgctccag aagccggtca agggaagcaa gcgtgacatc ggggaaaaaa
     4981 cggggcggtg tcgccggccg aagatagggg aagggttctt ccccttcaag atagactccg
     5041 gcggcttctc cggcaaagag agtcagcacc ccgtcgtcat cgaaaaggaa actctgatgg
     5101 tgcgccgcat gacctggagt atccacagcg aaaaaccctt tcggcagaga atctatcgaa
     5161 aggatcttct ccgctggaac aggaggcggc tcaccgtaga gcaaagccgt ttcaccaagc
     5221 gtttgcctgc tgcccttcca gagagctgat gggtcaagca gatgcggcac gcctttttca
     5281 ggggctgcaa caagagcgcc aaattctctg acgacaagcc ccgatgctcc ggcatgatca
     5341 atatgaacgt gggttaccaa cactgcgtcg acccgtccgt ttcctttgct gtgcaccgca
     5401 tcacgaagca gaggccacgt ggaaggcggg ccagggtcga caatgtatgt cctgtctctt
     5461 gcggtatctc gtagaagcca cgctgaaacg aaccgcctga aaccgggtct cgatagcggg
     5521 aggtcaacca gatgaaaatc catcacgccc tcctattccg aaataataac tttcattcca
     5581 atgaagacct tatcttttaa ttcttcgaga tcctcgttcc taaggcgaac acacccttga
     5641 gtgatcatcc tcccgataga atcaggatcg tgggtaccgt gaatgccgat t
//
LOCUS       DAOSWW010000046         5613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_9582_length_5613_cov_4.242173, whole genome shotgun sequence.
ACCESSION   DAOSWW010000046 DAOSWW010000000
VERSION     DAOSWW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9582_length_5613_cov_4.242173"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PK442_01740"
     CDS             <1..559
                     /locus_tag="PK442_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOO86398.1"
                     /translation="TIFLGFLMAQRTQSVLIMVFSIVGGSLLGEWFDLDKRLNNLAEW
                     TKGKIRSKNEKFTEGFVAASLLFCVGSMAILGAIEEGMGEYPRLFITKSFMDLMASAA
                     MGASLGVGVVFSAFPVLVYQGALTLLAEGVQRVMSPEVIAEVSAVGGLMLLGIGLAIL
                     EIKRIKVINMLPALILAGILASFFR"
     gene            complement(648..1397)
                     /locus_tag="PK442_01745"
     CDS             complement(648..1397)
                     /locus_tag="PK442_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOO86399.1"
                     /translation="MNHPFMDIPITAAPSFSWEASRAVPIDECGEEMVAASLIPEKIL
                     VRPQYYLHMLEGAIPECYVRRSVLERLIEAADMLPDGRRLVLLDTWRSRNIQTTLFQK
                     FRSELREKMPLLDDSEITVLASQFVAPPSLHPERPSPHITGGAVDLTIVDKMGFCLPM
                     GTGFDETSEKSATVWYEKKFDSKEAMSEDETEALHNRRLLYYVMHKAGFTNYPDEWWH
                     YDYGNQNWALLRGKKHAIYGVTEPAFRWRSL"
     gene            complement(1422..2711)
                     /locus_tag="PK442_01750"
     CDS             complement(1422..2711)
                     /locus_tag="PK442_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO86400.1"
                     /translation="MGISMLFAFLLLMILGVPLYLSLLSTSLFGIVMLGDFSLLRVMS
                     QQFFGGMDVFSLMAIPFFILAGILMNKTGLTDRLLDFSRLLVGSVRGGLGYVNVVGGI
                     ILAGVNGSAAADASALGSILIPAMVKDGFSPPYAAGLTAGSALIGPIIPPSIFMIIYA
                     TMTNTSIGGLFAAGILPGILLGGAFMGMNYVYSHRYQVPVADFEEVRKMKKEILKRSL
                     VALFAPVIIIGGIITGVVTPTESGALAVAYCLVAGTLYTRQMTFRVLAESLYETVRLT
                     SSIFLIMGAAMVIGWLLKWEQVPQKFASIILQAGLLDKPWLLMVVLSAIIFPVGMFME
                     EVSTLALLTPIFAPIALKAGIDPFHFGVVMTLNVTIALITPPMGACNYIVAAVGRVKL
                     SDVFFHIWPFIGVALLVLLAIISFPPLTTLIPRMLNL"
     gene            complement(2716..3087)
                     /locus_tag="PK442_01755"
     CDS             complement(2716..3087)
                     /locus_tag="PK442_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOO86401.1"
                     /translation="MVFRYLLKSSLIWTEEAARFSLVWMVMMGALGAAVKGDHMVIDF
                     VVPRFPVALRRLIGWGRFLLTILILSLMIYLGTVNAVQMWNMRTMALNIPKTIPLLSL
                     PVGFLLLMAGTILMQFDRGRE"
     gene            complement(3244..4287)
                     /gene="dctP"
                     /locus_tag="PK442_01760"
     CDS             complement(3244..4287)
                     /gene="dctP"
                     /locus_tag="PK442_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO86402.1"
                     /translation="MKFSKVSALFVLFALFAVMVPGYAEAAVEVKMSYNGPGDVENNA
                     VHLYAVNFKKFVEEGTGGEVTIALFPDSQLGNEEERMELLSKQGMNQPIVNVASFAGV
                     APVFPEIYATAVPFMFDGFKAAHIFHDESRFWKKAQEEFHKRTGAYLLEAVEEGGFLA
                     FSNNKREIRKAEDFAGLKFRGMDEGQVILCKSFGASGTPIPWTELYMALKTGVVDGQM
                     NPAMYIKMGSLQEVQKYLTLANIQYSDQFLVVNGDFMDSLSEEHKKAVVEAAKKANDL
                     NRKAMAEQDQKDIDFLVEKGMIAYSPTEEEMETFRSAAQPAYVEWLKSKAGEDWLALA
                     LECAKNANETAKK"
     gene            complement(4452..>5613)
                     /locus_tag="PK442_01765"
     CDS             complement(4452..>5613)
                     /locus_tag="PK442_01765"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOO86403.1"
                     /translation="FRVLYSRWASRVMAYAARALELCWRLFPREDFESPFKNLLSKAP
                     SNIVEFVNGKRVYDIFVTQMRSDLLRAGAHYAVSALFSGDEGDVRANLGDVFYAYRVL
                     SCSLERKDEGARRYALGYLRIRSEITRDEKELFVAALYRGGRNVLCGVAEKPEPEDGN
                     GIREKIEEALRSEDEEGLVSFFGHNTYSLRHLFKDEQRRILNLIIAEDVEAVVSMLRK
                     TISDSSELLSFLSELAMPIPEAFRSAAEVTLNDELKRAFEADALDLDFVSQRIDEGER
                     WGAGVDVLFLRHAAMRRLERLFLLLEREPFEENTLRSIIELLSFLKKRGWEINLWDEQ
                     NTFAGIIASNEDLSSCGLLYETLGKMLLVRHSRVESDFADRVTPRSRQKGVV"
BASE COUNT         1339 a         1600 c         1493 g         1181 t
ORIGIN      
        1 cacgatcttt ctcgggtttc tgatggcgca gaggacgcag agcgtcctca tcatggtctt
       61 cagcatcgtc ggcggctcgc ttctcggcga atggttcgac ctggacaaac gtctgaacaa
      121 cctggcggag tggaccaagg gaaagatacg ctcgaaaaac gaaaagttca cagaggggtt
      181 cgtcgccgct tcgcttctct tctgcgtcgg ctcgatggcg atcctcggcg ccatcgaaga
      241 ggggatggga gagtatccca ggctcttcat cacgaaatcc tttatggatc tgatggcctc
      301 cgcggcgatg ggcgcctctc tcggagtcgg ggttgtgttt tccgccttcc ctgtccttgt
      361 gtaccaggga gccctgacat tgcttgcgga gggcgtacag cgtgtgatga gcccggaggt
      421 gatcgcggag gtgagcgcgg taggcgggct tatgctcctg ggcataggac tcgcgatact
      481 ggagatcaag cgcatcaagg tgatcaacat gcttcccgcc cttatattgg cggggatcct
      541 ggcgtcattc tttcgctgat ccgcttttcg ctgacctgct aaaaagaaac ggaaggccgg
      601 agtatggatt ctccggcctt ccgtttcttt tttagctctt cttcgtatca gagggaacgc
      661 cagcggaacg cgggttctgt tacgccgtag atcgcatgtt tcttcccgcg gagcagcgcc
      721 cagttctgat tgccgtagtc gtagtgccac cactcgtcgg ggtagttggt gaatcccgcc
      781 ttgtgcatca cgtaatacag cagccgccgg ttgtgcaggg cctccgtctc atcctcggac
      841 atggcctctt ttgaatcgaa ttttttctcg taccacacag tggccgactt ctcggaagtc
      901 tcgtcgaacc cggttcccat cggcagacag aagcccattt tatcgacgat ggtcaggtct
      961 accgctcccc ccgtgatatg aggcgagggg cgctcggggt gcagcgacgg cggcgcaaca
     1021 aactgagagg cgagaacagt aatttccgaa tcatcaagaa gcggcatttt ctcacggagt
     1081 tcggagcgga atttctgaaa aagggtcgtc tggatattgc gggaacgcca cgtatccaag
     1141 agcacaagcc ggcgcccgtc gggcagcata tctgctgctt caataaggcg ttcgagcacc
     1201 gaacggcgta cgtagcactc cgggatggcg ccttcgagca tatggagata gtactggggc
     1261 cgaacaagga tcttctccgg tataaggctt gcggccacca tctcctcgcc gcactcatct
     1321 atcggcaccg ctctcgaagc ttcccacgaa aagcttggag cggctgtaat cggtatgtcc
     1381 atgaagggat gattcatttt tcttctcctt ccatttccgt gttacaggtt cagcatccgc
     1441 ggaataagcg tagtcagcgg aggaaatgat atgatcgcga gcagaacaag cagggcgacg
     1501 ccgatgaaag gccagatgtg gaaaaaaacg tccgaaagct tcaccctccc cacggctgcg
     1561 acgatatagt tgcatgctcc cattggggga gtgatcagcg caatcgttac attgagcgtc
     1621 atcacaactc cgaagtgaaa ggggtctatg cccgctttca gcgcgatagg cgcaaaaata
     1681 ggggtaagaa gcgccaaagt tgaaacctct tccatgaaca tgcccaccgg aaagatgatg
     1741 gcgctgagca cgaccataag gagccacggt ttgtccagaa gccccgcctg gaggatgata
     1801 gaagcaaact tctgcggtac ttgctcccac tttaaaagcc atccgataac catggccgct
     1861 cccatgatga gaaagatcga gctggtcagt cggacggtct cgtagagcga ctccgcgagt
     1921 acccggaacg tcatctgccg cgtgtacagc gttcctgcga ccaggcaata cgcaaccgcg
     1981 agcgcgccgg attccgtcgg ggtgacgacc cctgtaataa ttcctcctat gataatcacg
     2041 ggcgcgaaaa gtgcgaccag cgaacgtttc aggatctctt ttttcatttt acgaacttct
     2101 tcaaaatcgg cgacgggaac ctggtaccgg tgagagtaga cgtagttcat tcccataaaa
     2161 gctcctccca gcaggatacc gggcaggata ccggcggcaa acagcccgcc tatcgacgta
     2221 ttcgtcatgg tagcgtagat aatcataaaa atactcggag ggataatcgg accgatgagc
     2281 gcgctccccg ccgtcagccc cgccgcgtag ggaggagaga aaccgtcttt gaccatggcg
     2341 ggaatcagaa tggaaccgag ggcggacgca tccgcggcgg cagaaccgtt caccccggcg
     2401 aggatgatgc cgccgacgac gttgacgtac cccaaaccgc cccgaaccga cccgacgagc
     2461 agacggctga aatcaagcag tctatccgtc aaccccgtct tgttcattaa aataccggca
     2521 aggatgaaga acgggatagc cattaaggag aacacgtcca taccgccgaa aaactgctga
     2581 ctcatgacac gcaataaaga aaaatcgcca agcatcacta tcccgaacaa agaggttgag
     2641 agtagcgaga gatagagcgg cacgccaagg atcatcagga gtaaaaaagc gaaaagcatc
     2701 gatatcccca taggatcact ccctccctcg gtcgaactgc atcagtattg tacccgccat
     2761 caagagaaga aaccctacag gcagtgaaag aagcgggatc gtctttggta tattgagcgc
     2821 catcgttcgc atgttccaca tctgcacagc attgaccgtg ccaagataga tcatcagtga
     2881 aaggatgagt attgtcagga gaaagcgccc ccagccgata agccgccgga gggcgacggg
     2941 gaaccgggga accacaaagt cgatgaccat atgatcccct ttcaccgccg ctccgagggc
     3001 ccccatcatg accatccata ccagagagaa gcgcgccgcc tcttccgtcc aaatcaagga
     3061 acttttgagc aggtacctga aaaccactcc gacgagtaca tctcctatat taaatgcaag
     3121 aagaagacat gctgcccagg cgcttatccg ctcaaggcga tttgtccaaa cagccagaac
     3181 gctctcatgc tctttggagg aatcggtttc aggagacagg gtgaaaaagg acatggagaa
     3241 cctttacttt ttcgccgtct cgttggcgtt ttttgcgcac tcaagggcca gagcgagcca
     3301 gtcctctccc gccttcgatt tgagccactc cacgtacgcc ggctgcgccg cgctgcggaa
     3361 ggtctccatt tcttcttccg tcggcgaata ggcgatcatg cccttttcga ccaggaagtc
     3421 gatatccttc tgatcctgct cggccatggc cttgcggttc aggtcgttcg ctttctttgc
     3481 ggcctcgaca acagcttttt tgtgctcctc ggaaagcgag tccatgaaat cgccgttgac
     3541 tacaaggaac tggtcggaat actgtatgtt ggcgagagta aggtatttct gtacctcctg
     3601 aagacttccc atcttgatgt acatggcggg gttcatctga ccgtccacga cgccggtctt
     3661 cagcgccatg tagagctccg tccacggaat aggcgttccg ctggctccga aagatttgca
     3721 aagaatgacc tgaccttcgt ccattccgcg gaatttcagc cctgcaaaat cctccgcttt
     3781 ccgaatctcc cgcttgttgt tggaaaacgc gaggaaaccg ccctcctcaa cagcctcaag
     3841 gaggtacgcg ccggtacgtt tatggaactc ttcctgggct ttcttccaga aacggctctc
     3901 gtcgtggaaa atatgcgcgg ccttgaagcc gtcgaacatg aagggaacag ctgtcgcgta
     3961 gatctccggg aagacgggag ccactccggc gaaggacgcc acgttgacga tgggctggtt
     4021 catcccctgc ttcgagagaa gttccatccg ctcctcctcg ttgcccagct ggctgtccgg
     4081 gaagagcgcg atggtaactt caccgccggt gccctcttcc acgaatttct tgaagttcac
     4141 cgcgtacaga tgcaccgcgt tattctccac atcgccgggg ccgttgtagg acattttgac
     4201 ctcgacagcc gcctcggcgt accccggaac catgacggca aacagcgcga acagaacgaa
     4261 aagagccgat accttactga acttcattgc acataccctc ccttgaaaaa tacagaacac
     4321 ccctccggca ggacacccgg agagcggaaa acggagagac catctctccg aaccattaaa
     4381 tacccggata ctattacacc acaaaaaagc gattcgatgc aggactttcg tctcatcaag
     4441 agaaaagcgc gtcacaccac ccctttctgc cgcgaacggg gagttaccct atccgcaaaa
     4501 tcgctttcca cacgggagtg ccgaaccaac agcatctttc ccaatgtctc gtagagcagg
     4561 ccgcaggatg aaagatcctc gttcgacgcg atgattccgg cgaatgtgtt ctgctcatcc
     4621 cacaaattga tctcccaacc tctttttttt aagaaagaaa gaagctctat tatgctccgc
     4681 aaggtgtttt cttcaaacgg ctcccgttcc agcaggagaa agaggcgttc gagacgccgc
     4741 atcgcggcgt gacgcagaaa aaggacatcg actccggcgc cccagcgctc tccttcgtct
     4801 atccgctgcg acacaaaatc aagatcaaga gcatccgcct cgaaggcgcg cttcagctcg
     4861 tcattaagcg tcacttcggc agcgctccgg aacgcctctg gaatcggcat cgcgagctcg
     4921 gagagaaagg agaggagttc cgatgagtcc gaaatcgtct tcctgagcat tgaaacgacg
     4981 gcctcgacat cctcggcgat gatcaaattt aggatacgcc tttgctcgtc tttgaagaga
     5041 tggcgcagtg aataggtgtt gtgcccgaag aaggaaacca gcccctcttc atcctcgctt
     5101 cgaagagcct cctcgatctt ctcacggata ccgtttccgt cttccggctc cggcttctcg
     5161 gccacgccgc acagcacgtt ccgtcctccg cggtagagag cggcgacaaa aagctctttt
     5221 tcatcccgtg taatttcgga acggattcga agatagccga gagcataccg gcgggctcct
     5281 tcatcttttc gttcaaggga acacgacaaa accctgtaag catagaaaac gtctccaaga
     5341 ttcgcacgca catccccctc gtcgcctgaa aagagggcgg agacggcgta atgcgctccg
     5401 gcgcggagca gatccgaacg catctgcgtg acaaaaatat cgtagacgcg ttttccgttg
     5461 acgaattcaa cgatgttgct gggagctttt gatagaaggt tcttgaaagg cgattcaaaa
     5521 tcctcccggg gaaagaggcg ccagcacagt tcaagcgcgc gggcagcgta ggccatgacc
     5581 cggctcgccc atcgcgagta gagcacccgg aag
//
LOCUS       DAOSWW010000047         5561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_9722_length_5561_cov_4.460225, whole genome shotgun sequence.
ACCESSION   DAOSWW010000047 DAOSWW010000000
VERSION     DAOSWW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_5561_cov_4.460225"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="PK442_01770"
     CDS             <1..1973
                     /locus_tag="PK442_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO86404.1"
                     /translation="LWNFKDDLGTLSAKTSHVARFVPWDWIVGTGVYLDDIHMEIASW
                     RRRFTLLGLFAVLGSGGLSLFLSIRAVLLGRKAALFVVADRSLREKTRTLIQTEKQLA
                     IITSVYDHASEGVVITESDGTILRVNRAFSEITGFSKDEAIGRNPRILKSNTHDGEFF
                     ERMWSDLLEKKRWSGEIWNRRKNGEAFPAMLNIVACSDDSGETTHYIGVFQDLSELHR
                     SRDSLRHETFHDSLTGLPNRFLFSDRLAMALGRAKENAVLVSVIILGLDRFSSINKTL
                     GYPAGDLILKEAGSRIEAVVGETGTAARFGGDEFVILIPKSSDLHSVLRTTHRMLASL
                     RAPFSIEGESLRLTASAGVALYPRDGDAPENLLQNASLAMERAKKEGGDSYRLFSETL
                     DRKVQQRIRLEGDLRNAIDEEAFFIHYQPKISMIQGKTLSVEALLRWTNRENESIPPD
                     VFIPLAEDIGEINSLGRFVLERSCLQAKEWRGEGRDIVMCVNVSAKQLASERFVHSVL
                     GALERTGTPARTLELEITESAFMSNPEYATRVMEELKERGLRFTLDDFGTGYSSLSYI
                     RSLPLSGIKIDRSFTADLDNPKTRAIVSTIMHMAGELNLELTVEGIETTAELNTIRSL
                     APRGGDILLQGYLFSRPIPPDTFSSLFDRDFM"
     gene            2077..3747
                     /locus_tag="PK442_01775"
     CDS             2077..3747
                     /locus_tag="PK442_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HOO86405.1"
                     /translation="MYHKMFHRSAYHRAVAVLMAAVSLFLHAPAHAEGAVSSPGEIPP
                     PKVIPAEAFSFDNVVKKAEELAARPFENPDGAVPDFLLNISYDQWRKIRFRPNMSLWR
                     NEKLPFEAQFFHPGLFYNRLVGINIVHDGGALRLPFSTNFFEYGDNAFASKTGSVPLD
                     FTGFRLHFPLNRDDYKDEVAVFLGASYFRAVARGTQYGLSARGLAVDTAMQSGEEFPY
                     FREFWIEKPAQDAEQIAVCALLDSPSLAGAYRFILTPGEETVMDVECMLFLRKDVQKI
                     GIAPLTSIYLYGEPENGRDGDFRPEVHDSDGLLYHSEEGEWTWRPLANPKRLAVLSAP
                     LQNPRGFGLMQRDWLFDHYQDLEARYEKRPSLWIEPLSEWGEGNVELVEIPTEQEIHD
                     NIVAYWVPAKTPETAEGRPKGDTPEYPRTMTFAYRMRWLQPHRPVHELGRVMATRIAA
                     GNSPEMMKFVLDFEGDELSELPAGAGLTSIVMVGDGARLIEKQLQKNEATGGWRLVFQ
                     VAPEAESGLKTLFPSPAGRPRIRLSALLKRGENIPEPLTETWTYDLHP"
     gene            3731..4087
                     /locus_tag="PK442_01780"
     CDS             3731..4087
                     /locus_tag="PK442_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86406.1"
                     /translation="MTSIRDTDRWRISRDRVLLYVRALNLPPYKGLDLAVESLKRTGP
                     SSAAESMESLRALLKEHGLDRGVLDSEGKHLASVPPLNRSVMVPEELDRVPWRTSFVQ
                     FLRRWKNDLFGKGCAR"
     gene            4084..>5561
                     /gene="mdoH"
                     /locus_tag="PK442_01785"
     CDS             4084..>5561
                     /gene="mdoH"
                     /locus_tag="PK442_01785"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HOO86407.1"
                     /translation="MTAFELDWSRSAGRRRLLLIFLVLAPTFIACGTMASILPSRGGS
                     PLEIVLLAVFAVLFAWISVGFWTALMGFFLLLQRRNRYSVSNVCEGCGFSIENPHART
                     AVLIPIYNEDVKRVFAGLKTVYDSLAATGSPDRFDFFVLSDTTDPDVWVQEEEGWRRF
                     CTEQDAFGRVFYRKRRSNAKRKSGNVADFCRRWGKNYRYMIVFDADSIMAGRTMVRMV
                     QMMEARPDIGILQTPPAGVNRETLLARAQQFANRVYGPMFAAGLHFWQLGDAQYWGHN
                     AIIRVEPFIRHCELPRLPGKGPLGGDILSHDFVESALMRRAGYGVWLAYDLDGSYEET
                     PPTLLDELKRDRRWCQGNLQHLRLIFSRGFFPAHRALFLNGILSYGSALLWLAFLLIS
                     SAQAVAEVYLEPVYFLDTKTLFPQWPVWRPHWALALLGSTAVLLFLPKFFSYALALLR
                     DPAARLYGGRLRLACGILLEVALSTLLAPVRMLHHSVRTGAS"
BASE COUNT         1037 a         1761 c         1709 g         1054 t
ORIGIN      
        1 atttgtggaa tttcaaggac gatctgggga cgctctcggc caagacatcg cacgttgccc
       61 gcttcgttcc gtgggactgg atcgtcggaa cgggcgttta cctggacgac atacacatgg
      121 agatcgcctc gtggcggcgg cgcttcacgc tgttgggact cttcgcggtg ctcggctcgg
      181 gcggtctttc gctctttttg agcattcgcg cggtcctctt ggggagaaag gcggcgctct
      241 tcgtcgtcgc ggaccgttcg ctccgcgaga aaacccgcac gctcatacag acggaaaaac
      301 agcttgcgat tatcaccagc gtctacgatc atgcgtcgga aggggtcgtc atcaccgaat
      361 ccgacggcac gattcttcgc gtcaaccggg cgttcagcga gattaccggt ttttcgaagg
      421 atgaagccat aggaagaaac cccaggattc tcaagtcgaa cacgcacgac ggagagttct
      481 tcgagcgtat gtggagcgac cttctggaga aaaaacggtg gtccggggag atttggaaca
      541 gacgaaagaa cggcgaggcg ttcccggcga tgctgaacat cgtcgcctgc agtgacgact
      601 ccggagagac aacgcactac atcggcgtct tccaggacct cagtgaactg caccgcagca
      661 gggacagtct tcgccacgag acgttccacg acagcctcac cgggctgccg aaccgtttcc
      721 tcttctccga caggcttgcg atggccctcg gcagagcaaa ggagaacgcc gttctcgtct
      781 ccgtgatcat actgggtctg gacaggttca gctcgatcaa caagacgctg gggtacccgg
      841 cgggcgacct catcctcaag gaggcgggat cgcgcatcga ggcagtcgtt ggcgaaacgg
      901 gaacggcggc acggttcggc ggcgacgagt tcgtcatcct catcccgaag agcagcgacc
      961 tccactcggt gcttcgaacg acacacagga tgcttgcctc gctgcgcgct ccgttctcca
     1021 tcgaaggaga gagtctccgc ctcaccgcca gcgcaggcgt ggcgctctat ccgagggacg
     1081 gagacgctcc ggagaacctc ctgcagaacg cctcgctcgc catggagcgc gccaagaagg
     1141 aggggggcga ctcgtaccgc ctcttctccg agacgctcga ccggaaggtg cagcagcgga
     1201 tacggctcga aggagatctt cgcaacgcga tcgacgagga ggccttcttc attcactacc
     1261 agccgaagat cagcatgata caagggaaaa ccttaagcgt ggaggctctg ctccggtgga
     1321 cgaacagaga gaacgagagc attccacccg acgtttttat ccctcttgcg gaggacatcg
     1381 gcgagatcaa ttcgctcgga agatttgtcc ttgaacgctc ctgcctccag gcgaaggagt
     1441 ggcgcggcga ggggagggat atcgtcatgt gcgtgaacgt ctcggcaaaa cagcttgcct
     1501 cggagcgatt cgtccactcc gtcctgggcg cgcttgaacg caccggaacg ccggcccgca
     1561 cgctggagct ggagatcacc gaatccgcct tcatgtcgaa ccccgagtac gcgacgcgtg
     1621 taatggagga gctcaaggag cgcgggctgc gcttcaccct cgacgacttc ggcaccgggt
     1681 actcctcgct ctcctatatc cgcagcctcc cgctctcggg catcaagatc gaccgctcct
     1741 tcaccgccga cctcgacaat ccaaagacgc gcgccatcgt ttcgacgata atgcacatgg
     1801 cgggcgaact caacctggag ctcaccgtcg aaggaatcga aacgacagcc gaactcaata
     1861 ccatccgttc gctggccccg cgcgggggag atattctgct ccaggggtat ctcttctctc
     1921 gtccgattcc gccggatact ttttcatcac tcttcgacag ggacttcatg taaaaagtct
     1981 ctcttttcac cccccttgcg ctccatccaa aaatgactat gatgtatctg ctttcacaac
     2041 cggtccggat actccggcca aggagggtct tgtgtcttgt atcacaaaat gtttcacaga
     2101 agcgcctacc atcgtgccgt cgccgttctc atggcggcgg tgtctctttt cctccacgct
     2161 cccgcgcacg ccgagggggc tgtttcgtcc cccggcgaga ttcccccgcc gaaagtcatc
     2221 ccggccgaag ccttttcttt tgacaacgtg gtgaagaagg ccgaagaact cgccgcccgt
     2281 ccctttgaaa atcccgacgg ggcggttccc gactttttgc tcaacatcag ctacgaccag
     2341 tggcgaaaga tccgcttccg cccgaacatg tccctgtggc ggaacgagaa gctgccattc
     2401 gaggcgcagt tcttccaccc cggcctcttc tacaacaggc tcgtcggaat caacatagtc
     2461 catgacggag gggcgctgcg acttcccttc tccacgaact tcttcgagta cggagacaac
     2521 gccttcgcgt cgaaaacggg gtcggttccg ctggacttca ccggattccg gctgcacttc
     2581 cccttgaaca gggacgatta taaagacgag gtcgccgtgt tcctcggcgc gagctacttc
     2641 agagcggtgg cgcgcggcac gcagtacggt ctctccgcgc gcggactcgc cgtcgacacc
     2701 gcaatgcagt ccggcgagga atttccgtac ttccgggagt tctggataga aaaacctgcg
     2761 caggacgccg aacagatcgc cgtgtgcgcg cttctcgaca gtccgagtct tgccggggcc
     2821 taccgtttta ttctgactcc cggcgaggag accgtcatgg acgtggagtg catgctcttc
     2881 ctgcgaaaag acgtgcagaa gatcggcatc gcgccgctca cgagcatata cctctacggt
     2941 gaaccggaga acggccgcga cggcgacttc cgtcccgagg tgcacgattc ggacggactg
     3001 ctgtaccaca gcgaagaggg ggagtggacg tggcggccgc tggcgaatcc gaagcgcctc
     3061 gccgtgctct ccgcgccgct tcaaaacccg cgcggtttcg gtctgatgca gcgggactgg
     3121 ctcttcgacc actaccagga ccttgaggcg cggtatgaga aacgcccgtc gctctggata
     3181 gagccgctca gcgagtgggg cgaggggaat gtggaacttg tggagatccc gaccgaacag
     3241 gagatccacg ataatatcgt cgcctactgg gtccctgcga agacgcccga aaccgccgag
     3301 ggccggccaa agggcgacac gccggagtat ccccggacga tgacattcgc ctacaggatg
     3361 cgctggcttc agccgcaccg cccggtccac gaactcggcc gggtcatggc gacgcgcata
     3421 gcggcgggga actccccgga gatgatgaag ttcgtcctcg acttcgaagg ggacgagctt
     3481 tcggaactcc cggcgggagc gggcctgaca agcatcgtga tggtcgggga cggcgcgcgc
     3541 cttatcgaga aacagcttca gaagaacgag gctaccggag ggtggcggct ggtcttccag
     3601 gttgcccccg aagccgagag cggtctcaag acgctctttc cttcgcccgc cggccgcccc
     3661 cgcatccgcc tctccgcgct gctgaagcgg ggggagaata tcccggaacc gctgacggag
     3721 acctggacct atgacctcca tccgtgatac cgaccgctgg cgcatctccc gcgacagggt
     3781 gctgctctac gtgcgcgcgc tcaacctgcc gccctacaag gggctcgacc tcgccgtcga
     3841 aagcctgaaa cgcaccggcc cgtcctccgc ggcggagtcg atggagtcgc tccgcgcgct
     3901 tttgaaagag cacgggctcg accggggcgt gcttgactcc gaagggaagc atctcgcctc
     3961 cgtcccgccg ctcaaccgga gcgtgatggt tcccgaggag cttgaccgcg ttccgtggag
     4021 gacgtcgttc gtccaattcc tccgacgctg gaagaacgac ctttttggaa aggggtgcgc
     4081 ccgatgaccg cgttcgaact cgactggagc cgcagcgcgg gccggaggag gctgctgctg
     4141 atcttcctcg tccttgcgcc gacctttatt gcgtgcggga cgatggcgtc catcctgcct
     4201 tcgcgcggag gaagccctct ggagatcgtg ctgctcgccg tcttcgcggt gctcttcgca
     4261 tggatctcgg ttggcttctg gactgcgctg atgggattct tcctcctttt acagcggcgc
     4321 aaccgttact cggtctccaa cgtctgcgag gggtgcggct tttcgataga gaacccgcat
     4381 gcacggacgg ccgtcctgat accgatctac aacgaggacg tgaaacgggt cttcgcgggc
     4441 ctgaagaccg tctacgattc gcttgccgcg acggggtcgc ccgaccggtt cgacttcttc
     4501 gtcctgagcg acactaccga tccggacgtc tgggtacagg aggaagaggg ctggcgccgt
     4561 ttctgcacgg agcaagacgc cttcgggcgg gtcttctacc ggaagcgccg gagcaacgcg
     4621 aagcggaaga gcggaaacgt ggccgacttc tgcaggcgct gggggaagaa ctaccgctat
     4681 atgatcgtct tcgacgccga cagcattatg gcgggacgga cgatggtccg tatggtacag
     4741 atgatggagg cgcggcccga tatcggcatt ctgcagacgc cgcccgcagg agtgaaccgc
     4801 gagacgctgc tcgcgcgggc gcagcagttc gccaaccgcg tctacgggcc gatgttcgcc
     4861 gcgggactgc acttctggca gctcggcgac gcgcagtact ggggccataa cgcgatcatc
     4921 agggtagaac cgttcatcag gcactgcgag ctgccgcgcc tccccggaaa ggggccgctc
     4981 ggcggcgaca ttctgagcca cgacttcgtg gagtcggccc tcatgcgccg cgccgggtac
     5041 ggcgtctggc tggcgtacga cctcgacggc agctacgaag agacgccgcc gaccctgctc
     5101 gacgagctga agcgcgaccg gcgctggtgc cagggcaact tgcagcacct acggttgatc
     5161 ttttcgcggg gtttttttcc ggcgcaccgc gcgctcttcc tgaacggcat cctctcgtac
     5221 ggttcggcgc tgctctggct ggccttcctg ctcatcagct cagcgcaggc cgtggcagag
     5281 gtctacttgg agcctgtcta cttcctcgac acaaagacgc tcttcccgca gtggccggtc
     5341 tggaggcctc actgggcgct cgcgctcctc ggctcgaccg cggtgctgct cttcctcccc
     5401 aagttcttca gctatgcgct ggcgcttctg cgcgatccgg cggcgcgtct ttacgggggg
     5461 aggctccggc tcgcctgcgg cattctgctg gaggtggcgc tctccacgct gctggcgccg
     5521 gtgcggatgc tgcatcacag cgtccgcacc ggcgcaagca g
//
LOCUS       DAOSWW010000048         5543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_9763_length_5543_cov_3.059038, whole genome shotgun sequence.
ACCESSION   DAOSWW010000048 DAOSWW010000000
VERSION     DAOSWW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9763_length_5543_cov_3.059038"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PK442_01790"
     CDS             complement(<1..1032)
                     /locus_tag="PK442_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOO86408.1"
                     /translation="MKARNGATKTLQRRGGIRRYGALLSTVVFLWAAWAGCAHAENEA
                     KAYPPVTWTVTEASDNPSSPGENTLRGIIGKATDLDIVVFGPGIAEISLQGELVIDKV
                     LTIAGSATQQVTIRQTAAGARVLSVPSGKSAALQYLTITGGRRAGYGDGGGILNNGTL
                     AINHCTIVDNSTEGYAGGGISNEDRATLTIKNSLVKGNSASGDGGVWNGGTLTMEDCI
                     VEDNTCSYSGGGIGSNSTMTLKNCTIRNNRSTMRSHSGGGVYTIGTATMSNCTVQGNS
                     AATDGGGIYNRGDLILINCTVANNTAANGGGINSVGSTNLMLSSRVFGNTPDQIRGSY
                     TADNTCIVGS"
     gene            complement(1215..2108)
                     /locus_tag="PK442_01795"
     CDS             complement(1215..2108)
                     /locus_tag="PK442_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO86409.1"
                     /translation="MDTRILTTFRRIVTSGGFSSAANELGYAQSTVSVHIQTLEETLG
                     GPVFDRAGRKPVLTHLGKELLGLSEEMLRLEERVRNLASGTSEPSGELNVATGESVMV
                     SRLGEPLARFKQRYPHVVLRMRNSYCPAMIDWVLRGESDLAVLIMPPLNAPDLETRPL
                     RREEMVFVAAPGKDRNLLLDALGRGKLDECFIHTEQGCSYRVFIENFFRSKGLVPEKT
                     MELWSMEGIKKCVQSGLGISFVPLMYVRGALADGSLEQLAAPPLEESFVIQAAWRKTG
                     ALSPAAREFLAILEEDALSWR"
     gene            2233..3468
                     /locus_tag="PK442_01800"
     CDS             2233..3468
                     /locus_tag="PK442_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOO86410.1"
                     /translation="MKKNLNVWTLAGFLVGPVLGSGVVLLPPLALEKAGAGAFSAWVV
                     TLVLMGVFAGVCAALALRFPGDGGLTAAVGAAFGDRYRTLCGRLLAGAVCFGPAAVLL
                     MTGEFLGEIVPQFGGETAGAWFFLALCVALLLRRVSFAGMLALVCSTVIGAGFLCGSL
                     SVLFFGEEATALALTVPSPAAFGETLVLLFWAVIGWEILGNYTLEIESPKRSIPLAAG
                     IAFCAICAVYLAIAAALPRIGAGGTIPSMTDLLTPLFGSFASPLLAVFGILLCVCTYL
                     MVVGGVSRLVASMAERGELPRMLALRNKEGAPSRAIAALGCVHGAGLLLSSLSVLDVA
                     SLVGAANGLFLLNSLLAVAAAARLLRSPAVRTASAVLAACFLILFALADRTALLVSVT
                     VLLFSLKEASRGDRVAVSG"
     gene            complement(3520..5091)
                     /locus_tag="PK442_01805"
     CDS             complement(3520..5091)
                     /locus_tag="PK442_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOO86411.1"
                     /translation="MRKKEDAMERTSAERRNLFTKTGGLLAGIAAALLMASLFAFMPG
                     TGEAATWTVTNGGDGGTGALRSVIESAAAGDSVVFDSSVSIVNLNTYLFVNKALTITG
                     PVTIKQNGAGQRVLYVTGDCTFNNLTITGGNLGDPNSNGGAGIYNYSTLTMNNCTVTG
                     NTGGGGILNKQNLTMNSCTISGNAASGHLKAGGIASTATGSWEVSLTMTDCIVENNIG
                     SDRANSGGIYNGVDGTLVMTRSTVRNNECSSLAGGMYISAYSSTTLKNRSVVTGNRPD
                     QICCGYGASYTTDGTCTIGDASGTSATALSGFAEGASPAPRKTTGEPDVAAVERDLRS
                     PSSELFGAVKNLLESDLGGLPGDVSAGLSSIDATLYNAFAYEDVPLADASGDGELVIE
                     FTASWPQNVRYYAALAEWEDAAAGALAVKEYVIPERGVQFEIKPGQPLPDGVTPPDFY
                     EDGEGLMTWRNVIADNGPYDHNSAEGLVSFRAASIRAEAQTASSGGSGCSAVGAPPFA
                     LLLGAPLLLLRKKHP"
     gene            complement(5281..>5543)
                     /locus_tag="PK442_01810"
     CDS             complement(5281..>5543)
                     /locus_tag="PK442_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86412.1"
                     /translation="LQIFDNTPRVVKCFISCGGPVVALGTTEAVLELPHGLITRFEPI
                     PKDPERGLLFDFLDRGTPVLHLLFVRGICEDFGIPYERVAGQ"
BASE COUNT          842 a         1725 c         1769 g         1207 t
ORIGIN      
        1 gctgcccacg atgcaggtat tgtccgccgt gtaggagccc cggatctgat ccggtgtgtt
       61 gccgaacacc ctggaggaga gcatcaggtt cgtggagcct acggagttga tcccgccgcc
      121 gttggccgcg gtgttgttcg cgacggtgca gtttatcagg atgagatcgc cgcgattgta
      181 gatgcctcct ccgtccgtcg cggcgctgtt tccctggacg gtgcagttgc tcatcgtcgc
      241 cgtgccgatg gtgtagactc cccctccgga atgactgcgc atggtggacc ggttattccg
      301 gatcgtgcag ttcttcagag tcatagtgct gttgctgcct atgccgccgc cgctgtagga
      361 gcatgtgttg tcctcgacga tgcagtcctc catggtcaac gttcctccgt tccagacgcc
      421 tccgtctccc gaagcggaat tccccttgac aagcgagttc ttgatcgtca gcgtggcgcg
      481 gtcttcattg gagatgcctc ctcccgcata accttcggtc gagttgtcca cgatggtgca
      541 gtgattgatc gcgagcgttc cgttgttgag aatgccgccg ccgtcgccgt atccggcccg
      601 tctgcctccc gtgatcgtca ggtactgcag ggccgcgctc tttcccgagg gaacggagag
      661 caccctggcg cccgccgccg tctgccgtat cgtgacctgt tgcgtggcgg agcccgctat
      721 cgtcagtacc ttgtcgatga ccaactctcc ctggagcgaa atctccgcga tgcccgggcc
      781 gaagacgacg atatcgagat cggtcgcctt cccgatgatt ccgcgcagcg tgttctcgcc
      841 gggagaggag gggttgtcgg aagcctccgt caccgtccac gtcaccggag gataggcttt
      901 tgcttcgttc tccgcgtgcg cgcaccccgc ccatgccgcc caaaggaaca cgacggtcga
      961 aagcaacgcc ccgtaccgtc gtattcctcc tcttctttgc agtgtcttcg ttgctccgtt
     1021 gcgcgccttc attcctccta ccacccttct tgttgcaaaa tctcccctgg tcccgggagt
     1081 tgaaagcccg tatccatagc gtcgaaaaat tcacccttat gccaaaagca ataataaacc
     1141 tttacaccgt gtgagagtca aggggaagtt ttcatgttcg agcgaaagta cacggcgctc
     1201 acgatcccgc ttcctcatct ccaggaaagg gcgtcctctt cgaggatggc gaggaattcc
     1261 ctcgcggccg gggagagcgc ccccgttttt ctccatgcgg cctggatgac gaagctctcc
     1321 tcaagcggcg gcgcggcgag ctgttcgagg gacccatcgg cgagggcgcc gcggacgtac
     1381 atgaggggga cgaaggagat gccgaggccg ctctggacgc actttttaat tccctccatg
     1441 ctccacagct ccatcgtctt ttcgggcacg aggcctttgc tccggaagaa gttctcgatg
     1501 aagaccctgt agctgcaccc ctgctccgta tggatgaagc actcgtcgag cttcccccgg
     1561 ccgagcgcgt cgagcagcag gttccggtct ttccccggag cggcgacgaa gaccatctct
     1621 tcccgccgca gcgggcgcgt ctccaggtcc ggcgcgttga gcggcggcat gatgagcact
     1681 gccagatcgc tctcgccgcg cagaacccag tcgatcatcg ccggacagta cgagttgcgc
     1741 atccgcagga cgacatgagg atatcgctgc ttgaagcggg cgagaggctc tcccagcctt
     1801 gacaccatga cgctctcccc cgtcgccacg ttcagttcgc ccgagggttc ggatgtgccg
     1861 gacgccaggt tccggacgcg ctcctccagg cggagcatct cttcggagag cccgagcagc
     1921 tccttcccca gatgcgtcag gaccggcttt ctgcccgctc tgtcgaagac ggggccgccg
     1981 agcgtctcct ccagcgtctg aatatggacg gagaccgtcg actgcgcata ccccagctcg
     2041 ttcgccgcgc tggagaagcc gccgctcgtc actatcctgc ggaacgtggt taaaattctg
     2101 gtatccatgg cgcgcctccc gaaggcctct ttcgaggtgt gatatcgttt ttatcgatga
     2161 gaaccatctt ttatttccat tttaccgatt ttacttcctt ctgtatagtg ggcgcgaaag
     2221 gagagggtat tgatgaaaaa aaatttgaac gtatggacgc tcgcggggtt tctcgtcgga
     2281 cctgttctgg ggtcgggggt cgtcctgctg ccgccgctcg cgctggagaa ggcgggcgcc
     2341 ggcgcgtttt cggcgtgggt ggtcacgctg gttctgatgg gggtgttcgc cggggtgtgc
     2401 gcggcgctcg cactccgttt tccgggggac ggggggctga cggcggccgt gggcgcggcg
     2461 ttcggcgacc ggtaccggac gctctgcgga cgcctgctcg ccggggctgt ctgcttcggt
     2521 ccggcggcgg tgctgctgat gacgggagaa ttcctggggg agatcgtccc gcaattcggc
     2581 ggcgaaactg ccggggcgtg gttctttctg gcgctctgcg tcgcgctgct gctgcggcgg
     2641 gtctccttcg cgggaatgct ggcccttgtg tgttccaccg tcatcggggc gggcttcctc
     2701 tgcggaagcc tctccgtcct cttcttcggg gaggaagcaa cggcgcttgc gctgaccgtt
     2761 ccgtcccccg ctgccttcgg ggagacgctg gtgctcctct tctgggccgt gatcgggtgg
     2821 gagattctgg gaaactacac gctcgaaatc gagtcgccca agcggagcat tcccctggcg
     2881 gcggggatcg ccttctgcgc gatctgcgca gtgtatctcg cgatagccgc cgcgctgccg
     2941 cgcatcgggg cgggcggaac cattccctcg atgacggatc tgctgacgcc cctcttcggg
     3001 agtttcgcct ctccgctgct cgcggtgttc ggcatattgc tctgcgtctg cacgtacctc
     3061 atggtggtcg gaggagtctc ccgcctggtc gcgtcgatgg ccgagcgcgg agagcttccc
     3121 cgcatgctcg ccctcaggaa caaggagggc gcgccgtcca gagcgatagc ggctcttggg
     3181 tgcgtccacg gggcggggct gctgctctcc tccctctctg tgctggacgt cgcttctctg
     3241 gtgggcgccg ccaacggact ctttttgctg aactcgctgc tggcggtcgc ggcggcggct
     3301 cgcctgctgc gctctccggc cgtccggacg gcctctgccg tcttggccgc gtgcttcctg
     3361 attctcttcg ctctcgccga caggacggcg ctgctggtct cggtgacagt gctgctcttc
     3421 tctttgaaag aggcgagccg aggcgaccgg gtggccgtct ccggatgaat gctcgaaggg
     3481 ggcgcgagaa aacgcttttc tccgcgcccc cttcgtgttt tacgggtgtt ttttccgtag
     3541 gagcaacagc ggcgccccca gcaggagcgc gaagggcggc gcgcctacgg ccgagcaacc
     3601 ggagcctccc gaggaggccg tctgcgcttc ggcgcgtatc gaggccgcgc ggaaggagac
     3661 gaggccctcg gcgctgttgt ggtcgtacgg gccgttgtcc gcgatcacgt tgcgccaggt
     3721 catcaatcct tcgccgtcct cgtagaagtc gggaggcgtt acgccgtcgg ggagcggctg
     3781 accgggctta atctcgaact ggacgccgcg ctcggggatg acgtactcct tcacggcgag
     3841 agctcccgca gcggcatcct cccactccgc cagcgcggcg tagtagcgga cgttctgcgg
     3901 ccacgacgcg gtgaactcga tcaccagctc gccatctccc gatgcgtccg cgagcggcac
     3961 gtcctcgtag gcgaaggcgt tgtacagcgt cgcgtcgatg ctcgaaagcc ccgccgagac
     4021 gtctccggga agtcccccga ggtcggactc caggagattc ttcacagcgc cgaagagttc
     4081 gctcgagggc gagcgcagat cccgctcgac cgccgcgacg tccggttcgc ccgtggtctt
     4141 ccgaggcgcg ggggaagctc cctcggcgaa tccggagagc gccgtcgcgc ttgttccgga
     4201 agcgtctccg atggtgcagg taccgtcggt ggtataggat gcgccgtagc cgcagcatat
     4261 ctgatccggc cgattgccgg tcacgacgct gcggtttttc agcgtcgtcg aggaataggc
     4321 gctgatatac atccctccgg cgaggctgct gcactcgttg tttctgactg tgctccgcgt
     4381 catgacgagc gttccatcga caccgttgta gatacctccc gaatttgccc tgtccgagcc
     4441 gatattgttc tccacgatac agtcggtcat cgtcagagac acctcccagg agccggtggc
     4501 ggtgctggcg attcccccgg ctttaaggtg tcctgaagcc gcgttcccgc tgattgtaca
     4561 gctgttcatg gtgaggtttt gtttgttcag gatgccgccg cctccggtgt ttccggtcac
     4621 ggtgcagttg ttcatcgtca gcgtcgaata gttgtatatc ccggcgccgc cgttgctgtt
     4681 cgggtcgccg aggttgccgc cggtgatcgt caggttgttg aacgtgcaat cgcccgtgac
     4741 gtagaggacg cgctgcccgg cgccgttctg cttgatagtg acgggaccgg tgatcgtgag
     4801 cgccttgttg acgaagaggt aggtgttcag attgacgatg gagacggacg aatcgaaaac
     4861 gaccgagtcg ccggccgcgg cgctctcgat aacgctcctc aaggcgcccg ttccgccgtc
     4921 gcctccgttc gtcaccgtcc atgtcgcggc ctctcccgtc cccggcataa aggcgaacag
     4981 cgacgccatg aggagtgccg ccgcgattcc ggcgaggaga cctcctgttt tggtgaatag
     5041 attccgtctt tctgcgcttg tccgttccat cgcgtcttcc ttctttctca tggaatatac
     5101 tccgcgcggt cgatgaacgt acgttttggc gccgtcgtcg acatgccgcc ggaatagtgg
     5161 tgcgtacgcg aggtatcata aacctttcca ctgtgtgaga gtcaagaggg aatttcgatt
     5221 tttcggagtt ttttattacg gtattcttgc ccgaagcgcg gggcggcgtt ctgaaagagg
     5281 ctactgcccc gcgacgcgct cgtacgggat gccgaaatcc tcgcagatgc cgcggacgaa
     5341 gaggaggtgg agcacggggg ttcctctgtc gaggaagtcg aagagcaggc cgcgttcggg
     5401 gtctttcggg atcggctcga agcgcgttat caggccatga gggagttcaa gtaccgcctc
     5461 ggtcgttccc agggcgacga cggggccccc gcagctgatg aagcacttca cgacgcgcgg
     5521 ggtgttgtcg aagatttgga ggc
//
LOCUS       DAOSWW010000049         5429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10056_length_5429_cov_4.089505, whole genome shotgun sequence.
ACCESSION   DAOSWW010000049 DAOSWW010000000
VERSION     DAOSWW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10056_length_5429_cov_4.089505"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1792
                     /gene="argS"
                     /locus_tag="PK442_01815"
     CDS             116..1792
                     /gene="argS"
                     /locus_tag="PK442_01815"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOO86413.1"
                     /translation="MADVTKSLRGIVENALRKLADEKGIPSSDLPEIAFEKPKREGQG
                     DVSTNCAMKLCKLFGEKPLDLAGKIVERLEENEFVAKVEVAAPGFVNFFLSLKWMEKV
                     LSEILLEGKRYGSSSLGEDKKVQVEFVSANPTGPLHVGHGRGAAVGDIIASILSFTGW
                     RVEREYYINDAGLQMDILGRSTQSRYFEVACVPEKAPFPEDGYKGEYIYDIARAVREL
                     EGDRFLSMPLEESRPFFKKFAADAILETIKKDLADFGVKFDVWFSEKSLYERDLVPSA
                     MQTLKERGFAFENEGALWFKSTDFTDEKDRVLIRSNGVPTYFASDITYHKEKFDRGFE
                     KVIDIWGADHHGYVPRMKAGVEALGRNAEDLQVLLIQFVNLLRDGQQIPMSTRSGQFV
                     TLRDVLEEVGVDATRFFFVMRRSDSHLDFDLELAKQASNDNPVYYAQYAHARICSVLR
                     EAEARDVRLPGADEVNMEALSGDEERRLLARLGSFPEEMEKASRELAPHILVTYVLDL
                     AGDFHSFYNACRILGEEEFVMKARLLLVQAAGTVLSTALGLLGVSAPERM"
     gene            1797..2099
                     /locus_tag="PK442_01820"
     CDS             1797..2099
                     /locus_tag="PK442_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HOO86414.1"
                     /translation="MLRLRWILLAALLILFAAIMGTSFYLEIQKINRLTVLVDERMAI
                     LVSMSRTVQELREKIAFYGTTEGLAHLAREQYNLSFPGELVFKIEKKKNSLPEGRQ"
     gene            2179..2463
                     /gene="rpsF"
                     /locus_tag="PK442_01825"
     CDS             2179..2463
                     /gene="rpsF"
                     /locus_tag="PK442_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOO86415.1"
                     /translation="MRPYEMMVLVSAELEDPKEEIAKVEEVVRSLEGEIVKTDFWGKR
                     RLAYPIDKKTEGFYALCSFKLKPDQLGELHRVLGLRPNIYRQMTIRLDEK"
     gene            2476..2964
                     /gene="ssb"
                     /locus_tag="PK442_01830"
     CDS             2476..2964
                     /gene="ssb"
                     /locus_tag="PK442_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOO86416.1"
                     /translation="MAKGFNKVVLMGNLARDPEVRYTAGKQAVANFPLAVNRTWKDKN
                     GELQESVDFIPVVVWGSTAENCERYLKKGSPAFVEGRLQVRSYEAKDGSGKRYVTEVV
                     AAEVIFLGSKKQDAPSDSFEDEGFGDSIRKDRRFSGGSSDDDFPMDISSVQGEEDADI
                     PF"
     gene            2998..3294
                     /gene="rpsR"
                     /locus_tag="PK442_01835"
     CDS             2998..3294
                     /gene="rpsR"
                     /locus_tag="PK442_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOO86417.1"
                     /translation="MNEGRGEGRGEGRPEGRGGAGPRGPRKGGKRRPKVCFFCVDKVE
                     HVDYKDVEKLRKYVSERGKIMPRRVTGNCAKHQRQLTEAIKRARYMALLPFSLE"
     gene            3379..4350
                     /locus_tag="PK442_01840"
     CDS             3379..4350
                     /locus_tag="PK442_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOO86418.1"
                     /translation="MTSTRSLVESALLAGFAVVLFLAAHFLPLIGVAFSLLAPAPLVI
                     LGLRHDLKKATLGLGVSTLLVASLLGPLSALFFVLGFGVLGVGLGFLAKRCEKGVEVL
                     LYGILLSLGSKLLLMVIAGKVTGINPFQLDGAEMQSMIDKIFLFYESTGMSKESIAAV
                     REQFAESLRLLPVIFPTILTMAAALDCYLSYTISSFVLKRVGGTPLPPLPPFSMWRFP
                     KSVFGALVASILLSLFGSQSGEWNFALRAGTNLRLLVNFLFLFQGLSLIWYFFSEKAR
                     VPFGRVLRTVAIIVVVLVPLFSTIAVMAGIGDMWLDFRRRFGRSDAS"
     gene            4347..4793
                     /gene="rplI"
                     /locus_tag="PK442_01845"
     CDS             4347..4793
                     /gene="rplI"
                     /locus_tag="PK442_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOO86419.1"
                     /translation="MKVILKQDVNKLGASGDLVEISDGYARNFLFPRNLADEATPERM
                     KEWKDKEAARKKKEVRLEKEARELQKKLSGRAVKVKASIGEKGRLFGSITAANVAESI
                     VSQLAVEVEKKNLRMPESVKQIGSYSFTVRLHPGIEAEMTLIVEGE"
     gene            4794..>5429
                     /locus_tag="PK442_01850"
     CDS             4794..>5429
                     /locus_tag="PK442_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HOO86420.1"
                     /translation="MSVQSSWDRMPPVSLEAERSALGACLMDREALNIVMEILQPEDF
                     YDTTHRAAFEVIFGMAQRDKPVDPLTFLEEIARQGKGDLFGGQAFVGSLIDSVPTTAN
                     VEYHAGIVRDKAIHRRLISAGNTIVKLGYTEEMDVDEALEEAERAVFEIAQKRNSTNF
                     RHVAEVLGKTFQQIEEQYNRSDQDVTGFNSGFIELDRLTGGFQPGSLNIVAA"
BASE COUNT         1193 a         1336 c         1618 g         1282 t
ORIGIN      
        1 aaacgatgca gcgggaagaa gtctcgccga agccgtccag cgatggatgt cctgggggac
       61 gaagaacctt cccatgcgtt cgcgctaaac gaagagaacc tatggaggtg gctatatggc
      121 ggatgtaaca aaatccctta gggggatcgt tgaaaacgcg ttgcgaaaac tggctgacga
      181 gaaggggatc ccttcctcgg acctgccgga gatcgcattt gaaaaaccta aacgcgaggg
      241 gcagggagac gtttcgacga actgtgcgat gaagctctgt aaactgttcg gcgaaaaacc
      301 tttagatctg gccgggaaga tcgtcgagcg tcttgaggag aacgaatttg ttgcgaaggt
      361 tgaagtagcc gctcccggtt tcgtcaactt ttttctctcg ctgaagtgga tggagaaggt
      421 tctgtcagag attctcctcg aggggaaaag gtacggttcc tcttctctcg gcgaagacaa
      481 gaaggtgcag gtggaattcg tcagcgcgaa tcctaccgga ccgcttcacg tagggcacgg
      541 caggggggcg gccgtcggcg atatcatagc gtccattctc tccttcacgg ggtggagggt
      601 agagcgggag tactatatca acgatgcggg cctccagatg gacattcttg gacgctcgac
      661 acagtctcgc tatttcgagg tcgcatgcgt tcctgaaaag gcccccttcc ctgaggatgg
      721 ctataagggg gagtatatct acgacatagc gcgcgccgtt cgcgagcttg agggggatcg
      781 ttttctctcc atgcctctgg aggaaagccg gccgttcttc aaaaaattcg cagccgacgc
      841 tattctggaa acgataaaaa aggacttggc cgatttcggt gtgaagttcg acgtttggtt
      901 ctcagagaag agtttgtatg agcgtgattt agtcccttca gctatgcaaa ccttgaaaga
      961 gcgcgggttt gctttcgaaa atgaaggagc gctctggttc aagtcgacgg acttcaccga
     1021 cgagaaggac agggtcctga tcagaagcaa cggagtcccc acgtacttcg cgtcggacat
     1081 aacgtatcac aaagagaagt ttgacagggg cttcgagaaa gtcattgaca tctggggagc
     1141 ggatcatcac ggatacgttc cgaggatgaa ggcaggcgtt gaagctcttg gaagaaatgc
     1201 cgaggatctt caggtgctgc tcatacagtt tgtcaatctc cttcgggatg gacagcagat
     1261 ccccatgtcc acgcgatcag gccagttcgt cacgctacgg gacgtactgg aggaagtcgg
     1321 agtggatgcc actcgctttt tctttgtcat gcgacgaagc gactctcacc ttgatttcga
     1381 tcttgaactc gcaaaacagg catcaaatga caacccggtg tactacgccc agtatgcgca
     1441 tgcacgtatc tgcagcgtcc tccgcgaagc ggaggctcgt gacgtgcggc ttcccggtgc
     1501 agacgaggta aatatggagg cgctctccgg agatgaagaa aggcgtcttc ttgccaggct
     1561 tggctccttt cctgaagaga tggaaaaagc ttcgcgtgaa cttgcgccgc atatattggt
     1621 cacctatgtt ttggatctcg ccggtgattt tcactccttc tacaacgcgt gccgcatact
     1681 cggcgaggag gaattcgtga tgaaagcgcg tctgctcctt gttcaggccg ccggtacggt
     1741 gctttcgacg gcgcttggcc tgcttggggt ttccgctccc gagaggatgt agtgatgtgc
     1801 ttcgcctccg ttggattctg cttgcggcgc ttctgattct ttttgcggct ataatgggga
     1861 catcgtttta tcttgagatc cagaagatca accgtctgac cgtccttgtc gacgagcgga
     1921 tggcgatcct ggtctcgatg agccgcactg tgcaggagct tcgggagaag atagcgtttt
     1981 acggcactac ggaggggctg gcgcaccttg cccgcgaaca gtacaacctc tccttcccgg
     2041 gagagctggt ttttaaaatc gaaaaaaaga agaattcctt gcctgaaggg agacaataat
     2101 gtataatccc atggcaagtt tttccctgcc cctttctttg aaaaggggcc agagtccaga
     2161 gggaggaggt gaatgagtgt gcggccatat gaaatgatgg tgcttgtgag cgccgaactg
     2221 gaggatccca aagaggagat cgcgaaggtt gaagaggtcg tgcgcagtct tgagggggag
     2281 atcgtcaaga ctgatttctg gggaaaaagg cgtcttgcct accccatcga taagaaaacg
     2341 gaaggttttt acgcattgtg cagcttcaag ctgaagcccg atcagcttgg agagcttcac
     2401 cgtgttcttg gtttgcgccc gaacatttat cgtcagatga caatccgtct tgacgaaaag
     2461 taaggaggcg gggtcgtggc gaaagggttc aataaagtcg tactcatggg gaatctggct
     2521 cgtgatcctg aagtgcggta taccgccgga aaacaggccg ttgcgaactt tccgcttgct
     2581 gtaaacagaa cgtggaagga taaaaacggc gaattgcagg agagcgttga ctttattcct
     2641 gttgttgtct gggggtccac tgctgaaaac tgtgaacgtt acttgaagaa agggagtccc
     2701 gctttcgtcg agggcagact tcaggttcgg agttacgaag ccaaggacgg ttcagggaaa
     2761 cggtacgtca ccgaagtggt tgccgccgaa gttattttcc tcggctcgaa gaagcaggac
     2821 gctccttccg attcgttcga ggacgagggg ttcggcgaca gcatccggaa ggatcgccgc
     2881 ttcagcggcg ggagttcgga cgacgacttc ccgatggaca tttcctcggt gcaaggcgaa
     2941 gaggatgcgg atatcccctt ttaggtgttt tttgaaggag gacagtgcac aatggcaatg
     3001 aatgaaggta gaggcgaagg cagaggcgaa ggcaggcccg aaggcagagg cggagccggt
     3061 cccagagggc ctcgaaaagg cgggaaacgc cggcctaagg tatgtttttt ctgcgtagat
     3121 aaagttgagc atgttgatta caaagatgtc gaaaagcttc gaaagtacgt gagcgagcgc
     3181 ggaaagatca tgccgcgccg tgtgacggga aattgcgcga agcatcagcg tcagctcacc
     3241 gaggcgatca aaagggcccg gtatatggct ctccttccgt tctctctgga gtagggcgcc
     3301 tatctgaaac gttcttttcg gggggaggag cctggcttct ccccccgtcc ttttagtttt
     3361 tcgaagggag gttgcggtgt gacttcgaca cgaagcctgg ttgaatcggc tcttctcgct
     3421 ggttttgcgg tggtgctctt tcttgccgcg cattttcttc cgcttatcgg tgtagcgttc
     3481 tctcttcttg cccccgcccc tcttgtaata ctggggctgc gccatgatct gaaaaaagcc
     3541 acgctggggc tcggcgtatc gacgctcctt gtcgcgtcgc ttctcgggcc gctctcggcg
     3601 ctctttttcg tgctcggctt cggcgtgctc ggagtgggac tcggttttct ggcgaaacgc
     3661 tgcgaaaagg gtgtggaggt tctcttgtac ggaattcttc tctctttggg gagtaagctt
     3721 ctcctgatgg tcatcgcggg aaaagtgacg gggatcaatc cgtttcagct cgacggcgct
     3781 gagatgcagt cgatgatcga caagattttc ctgttttacg agtcgactgg catgagcaag
     3841 gaatcaatcg cagcggttcg agagcagttc gccgagtctc tccgtcttct gcccgttatc
     3901 ttccctacca ttctgacgat ggccgccgcg cttgactgct acttgagcta caccatcagt
     3961 tctttcgtgc tcaagcgcgt tggaggaacg cctctccctc cccttccccc tttctcaatg
     4021 tggcgtttcc cgaagagcgt tttcggcgcc cttgtcgcgt ccattctcct ctctctcttc
     4081 ggctcgcaaa gcggcgagtg gaactttgct ctcagggcag ggaccaattt acgtcttttg
     4141 gtgaactttc tctttctctt tcaggggctc tctcttattt ggtatttctt ctcggagaaa
     4201 gctcgcgtcc ctttcgggcg ggttcttcga accgttgcta taatagtagt agtgctggtt
     4261 cctctttttt ccaccatagc ggtcatggcc ggaataggcg atatgtggct cgatttccgg
     4321 agacgtttcg gaaggagtga tgcttcatga aagtcatttt aaagcaagat gtaaataaac
     4381 tgggcgcatc cggcgatctt gttgagattt cggatggata tgcacgtaat ttcctgtttc
     4441 cgcgcaatct tgccgacgag gccactccgg agcgaatgaa ggagtggaaa gataaggaag
     4501 ccgccagaaa gaaaaaagag gttcggctgg aaaaagaggc gcgcgaactc cagaagaagc
     4561 tttcagggcg cgctgtgaag gtaaaggcga gtattggcga aaaaggccgc ctcttcggca
     4621 gcatcacggc cgccaacgtt gcggagagca ttgtctcgca gctcgcggtc gaggtggaga
     4681 aaaaaaatct ccgtatgccg gagtctgtga agcagatagg aagctattct tttaccgttc
     4741 gcctccatcc cggaatagag gcggagatga ccctcatcgt cgaaggcgag tagatgtccg
     4801 tccagtcttc ctgggaccgg atgccgccgg tctctcttga ggcggagcga tccgcgctgg
     4861 gcgcctgcct catggacagg gaggctctca atatcgttat ggagattctc cagccggagg
     4921 atttctacga cacgacccat cgagctgctt ttgaggttat cttcggcatg gcgcagcgcg
     4981 acaaacccgt tgaccccctc accttccttg aggagatcgc ccgccagggg aagggcgacc
     5041 tcttcggagg gcaggcgttt gtcggttcgc ttattgacag cgttcccacc actgcaaacg
     5101 tggagtatca cgccggtatc gtcagggata aggcgatcca ccgcaggttg atcagcgcgg
     5161 gcaatacgat agtaaaactc ggatacaccg aggagatgga tgtcgacgaa gcactggagg
     5221 aagcggaacg cgccgtcttc gagatagcgc agaagcgcaa ctccaccaac ttcaggcatg
     5281 tcgccgaggt gctcgggaag accttccagc agatagagga gcagtataac cgctccgacc
     5341 aggacgtaac gggcttcaac tccggtttta tcgagctgga ccgccttacg ggcggtttcc
     5401 agccggggag tctcaatatc gtcgcagcg
//
LOCUS       DAOSWW010000050         5341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10273_length_5341_cov_4.188422, whole genome shotgun sequence.
ACCESSION   DAOSWW010000050 DAOSWW010000000
VERSION     DAOSWW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10273_length_5341_cov_4.188422"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK442_01855"
     CDS             complement(<1..387)
                     /locus_tag="PK442_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86421.1"
                     /translation="MSSSGNSAHKGAGYMGTGKRKNSFLFFTEDASPDLRSLSLLLLV
                     AFALTVIFLKWFFEDRISGFLPGEASSRTYFALYPMKYVDEEGTVALRSRVGDTVSGV
                     LVRDGAAMDRLISKIEIMAEGDVSLLS"
     gene            complement(359..1369)
                     /locus_tag="PK442_01860"
     CDS             complement(359..1369)
                     /locus_tag="PK442_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOO86422.1"
                     /translation="MYIDVENKAEENGASLRELDFSDIETMARFAGNNDENLSEIEQR
                     YPVSLLVRGNKVAVRGPDEVAVDVAYGLLEQLHKIASKGHALHLADVRYAMDMIAEKG
                     AVDLAPLFSEVICTTARGKPIRAKTEGQRAYLETVRNNHIVFAVGPAGTGKTFLAVCH
                     AVTLLKASAISRIVLVRPAVEAGESLGYLPGDLREKVEPYVRPLYDAFYDLLSPERFL
                     RYVDKGVIEIAPLAYMRGRTLNDSFIILDEAQNTTPEQMKMFLTRLGFGSKAVITGDI
                     TQIDLPNGKKSGLIQVRDILEGIEGIEFFNLTHSDVVRHEIVQKVVRAYERYEQQREQ
                     RA"
     gene            complement(1392..2828)
                     /locus_tag="PK442_01865"
     CDS             complement(1392..2828)
                     /locus_tag="PK442_01865"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOO86423.1"
                     /translation="METKSEPQPKKESRQIRKVGITDTTLRDAHQSLMATRMKVEDMA
                     EIIERMDDIGFHSMEVWGGATYDSCMRFLDEDPWERLRTMRRLFKKTKLQMLLRGQNV
                     VGYRHYADDTVREFVKRAVGNGLDIIRVFDALNDLRNMEVAADQIKKEGAHLQLAFSF
                     TLSPVHSLDAFGKLAKDMKSMGADSICIKDMAGIVSPVETAALVKTIKKETGLPVQIH
                     SHYTSGMASMAYYAGLEAGADVVDCAISPFALGTSQPPVESMVAALKGGDFDTDIELE
                     KLVPIAMHFKKVRDKYSKIFVDLGGVDINVLIYQIPGGMYSNLVSQLKDQNALARLPE
                     VLKEVPRVREEMGYPPLVTPTSQIVGTQATLNVLVGERWKVIPNEVKSYFRGEYGKPP
                     AEVDPEIRKKVIGDKEPITCRPAELIKPELEDARKAIAPWILQPEDVLSYVLFPSVAK
                     DFLMKKFAREMKVDVGVAELVDGAGYPV"
     gene            complement(2960..3760)
                     /gene="mazG"
                     /locus_tag="PK442_01870"
     CDS             complement(2960..3760)
                     /gene="mazG"
                     /locus_tag="PK442_01870"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOO86424.1"
                     /translation="METRSGHVFEELLAVMARLRGENGCPWDREQTMETLRTYIVEEA
                     YELVDAISRGETPDIVEEAGDLLLQVVFLSRIAEDEGRFTIDDVLRGLTEKLVRRHPH
                     VFGDEKADTSGQVLRKWEEIKGVEKEQRKKKDTSLLAGVPEGLPPLAKAARIQSKAAH
                     VGFDWEKGNLAPLYDKVDEEIEEIRNAVETGSADRIEDEMGDLLFAAVNLARHLEVNP
                     DSALGRANRKFSDRFRIVESMVRETGRPWSSFSLDTLDTFWEQAKKSL"
     gene            complement(3764..>5341)
                     /locus_tag="PK442_01875"
     CDS             complement(3764..>5341)
                     /locus_tag="PK442_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO86425.1"
                     /translation="TKDQLVAISEILEDMERRFPMDRLLVGDVGYGKTEVALRAAFRA
                     VQGGKQAALLVPTTILAQQHYLSFTARLTGFPVRVAFLSRFLSRKEQKDVLERVAAGE
                     VDILIGTVRMLREDVAFKDLGLLIIDEEHRFGVMSKEKLKQARENVDVLMLSATPIPR
                     TLALSLKGLRSISVLNEAPHNRIPVVTTAGPWSASIVKGAVSRELERGGQVYYVTNRI
                     TRMPKRLAFLRRLFPDVAIGMAHGRMKEAELEETMMGFYNGKIQILLCTTIIESGLDI
                     PGANTIVIEDCQELGLAQMYQLRGRVGRREEAAYAYFLYPEGRPLAAETLERLDAITS
                     LNDEGAGYDLALQDLRIRGSGDLLGTSQHGSGRGIADSHLYCTLLEEEIGKIRGVVPS
                     AATVSAEIPCLVPSFYIPQESIRIALYRRLLRVGSLDELHDLEKEVKDRFGTLPESLE
                     NLFSISFLRSRGGFYGILSMECTRRETTLTGEGPLFDLLRTWKRWSGKEKKLSGPGGA
                     SGLRDVLLGLRQIKQVL"
BASE COUNT         1017 a         1651 c         1495 g         1178 t
ORIGIN      
        1 cgagaggagc gaaacgtccc cctccgccat tatctctatc ttcgagatga ggcggtccat
       61 cgcggcgccg tcgcggacaa gcactcccga gacggtatcg ccgaccctgg agcggagcgc
      121 aaccgtccct tcctcgtcca cgtacttcat cgggtacagg gcaaagtagg tccgcgaaga
      181 ggcctccccc ggcaggaagc ccgatatcct gtcttcaaag aaccatttca aaaaaataac
      241 ggtcagcgcg aaggcaacga gaaggagcag ggagagcgaa cgaaggtcgg gagaagcgtc
      301 ctcggtgaaa aaaaggaaag agttctttcg tttgccggtt cccatatatc ctgcgccttt
      361 atgcgcgctg ttcccgctgc tgctcatagc gttcgtacgc cctgacgact ttctggacga
      421 tctcgtgacg gaccacgtcc gaatgcgtga gattgaaaaa ctcaattccc tcgattcctt
      481 caaggatgtc cctgacttgt atcagtccgg acttcttccc gttcggcagg tcgatctggg
      541 tgatgtcgcc cgtgatgacg gctttggagc cgaagccgag acgggtaaga aacatcttca
      601 tctgctcggg cgtggtgttc tgcgcttcgt cgaggatgat gaagctgtcg ttgagcgttc
      661 ttccccgcat gtacgccagc ggggctattt cgatgacccc cttgtccaca taccggagaa
      721 agcgctccgg agagagcaag tcgtagaatg cgtcataaag cgggcgcacg tacggctcca
      781 ctttctcccg aaggtcgccg ggcaaatagc cgaggctctc ccccgcctcc acggcgggac
      841 gcacaagcac gatcctgctg atcgccgagg ccttgagcag cgtcacggcg tggcagacgg
      901 caagaaaggt cttcccggtt ccggctggtc cgacggcaaa gacaatatga ttattgcgga
      961 cggtctcaag gtacgcgcgc tgcccctcgg tcttcgcccg gatcggtttt cctctggccg
     1021 tggtgcaaat aacttcggag aagagggggg ccagatcgac cgcccccttc tccgctatca
     1081 tatccatcgc gtagcggaca tccgccaggt ggagcgcatg ccccttcgag gctattttat
     1141 ggagctgctc aagcaacccg tacgccacgt ccacggcgac ctcgtccgga ccgcgtaccg
     1201 cgactttatt cccccgtaca aggaggctca cgggataccg ctgttctatc tcggagagat
     1261 tttcgtcatt attccccgcg aaacgcgcca tagtctctat gtcgctgaaa tcaagctcgc
     1321 gcagagatgc tccgttctcc tccgctttgt tttctacatc aatgtacatt cttctccgat
     1381 gcacgctccc tttagacagg atatccggcg ccgtcgacaa gttccgcgac gcccacgtca
     1441 accttcatct cccgcgcgaa tttcttcatc aggaagtcct tggccaccga cgggaagaga
     1501 acgtagctga gcacgtcctc aggctggaga atccacggag cgatagcctt ccgcgcatct
     1561 tcaagctcag gtttgatcag ctccgcaggg cggcaggtga tgggctcttt gtcgccgatg
     1621 acctttttac ggatttccgg gtcgacctct gcgggaggct tgccgtactc gccgcggaag
     1681 tagctcttca cttcattcgg aatgaccttc cagcgctcgc cgacgagcac gttgagcgtc
     1741 gcctgcgtgc ccacgatctg gctggtgggc gtaacgagcg gggggtaccc catctcctcg
     1801 cggacgcggg gcacttcctt gagcacctcg ggaagccggg caagggcgtt ctgatccttg
     1861 agctggctca cgaggttgga gtacatgcct ccggggatct ggtagatgag cacgttgatg
     1921 tccacgccgc ccaaatccac gaagatcttg gagtacttgt cgcgcacttt cttgaagtgc
     1981 atcgcgatcg gcacgagctt ttcgagctcg atatccgtgt cgaaatcgcc gcccttaagc
     2041 gccgccacca tggactccac aggcggctgg cttgtgccca aggcaaacgg cgagatggcg
     2101 cagtcgacga cgtccgctcc cgcctcgagc cctgcgtaat aggccatgga ggccatgccg
     2161 ctggtgtagt gggagtgtat ctgcacgggg agtcccgtct ctttcttgat cgtcttgaca
     2221 agcgcggcag tctccaccgg gctgacgatg cccgccatgt ccttgatgca gatggagtcc
     2281 gcgcccatgg acttcatgtc ctttgcgagc ttgccgaagg cgtccaggga gtgcaccgga
     2341 gaaagggtga acgagaaggc aagctgcaga tgcgctcctt ccttcttgat ctgatcggcc
     2401 gcgacctcca tgttccgcag gtcgttgagc gcgtcgaaga cgcggatgat gtcgagtccg
     2461 ttcccgacgg ccctcttgac gaactcgcgc acggtgtcgt cggcgtagtg gcggtatccc
     2521 acgacgttct ggccgcgcag gagcatctga agcttcgtct tcttgaagag tctccgcatg
     2581 gtgcgcaggc gctcccaggg gtcttcgtcg aggaagcgca tgcagctgtc gtaggtggcg
     2641 cctccccaga cctccatcga atggaaccct atgtcgtcca tccgctcgat gatctcggcc
     2701 atatcctcga ccttcatacg cgtcgccatg agcgactggt gcgcgtcgcg aagcgtcgtg
     2761 tcggtaatgc cgacctttct gatctgccgc gactctttct tcggctgcgg ttcgctcttg
     2821 gtctccatag tcgtctcctt atgcgacgtg gaagatgctg cgggggccgt ctcgctcttt
     2881 ttcttgttcg gggtggtcat gtgcgcattt cctccatacg ttcaattttt tgtgttcctg
     2941 tacacgatca gtttccctgt tacagggatt ttttcgcctg ttcccaaaaa gtatccagcg
     3001 tgtcaagaga aaagctgctc catgggcgtc ctgtttcgcg caccatggac tcgacgatcc
     3061 ggaaccggtc ggagaacttc cggttcgccc ttccgagcgc cgaatcgggg ttgacctcaa
     3121 gatgcctcgc caagttcacg gcggcgaaga gcaggtctcc catctcatcc tctatcctgt
     3181 ccgccgagcc tgtctcgacg gcgttccgga tttcctctat ttcctcatct actttatcat
     3241 acaatggagc aagattccct ttttcccagt caaaaccgac atgcgcggcc ttcgactgaa
     3301 tacgcgccgc cttcgccagc ggcggcaatc cttccggaac tcccgcgaga agcgacgtgt
     3361 cctttttttt ccgttgctcc ttctccacgc ccttgatctc ttcccacttc cggagcacct
     3421 gcccgctggt atcggctttt tcgtcgccga agacatgggg gtgacgtcgg accagttttt
     3481 ccgtcagccc ccgcagaacg tcgtcgatgg taaaacgccc ttcatcttcc gcaatccggg
     3541 agagaaaaac gacctgcagc aaaagatcgc ccgcctcctc gacgatatcc ggcgtctctc
     3601 cccgcgaaat cgcgtctaca agctcgtacg cttcctcgac gatgtacgtt ctgagggtct
     3661 ccatcgtctg ctccctgtcc caggggcagc cgttctctcc ccgaaggcgc gccatgacag
     3721 ccagaagctc ttcaaaaaca tgtccggaac gagtctccat attctacagc acctgcttta
     3781 tttgtcgaag tccgaggaga acgtcacgca acccgcttgc ccctccggga ccggagagtt
     3841 ttttctcctt gccggaccag cgtttccacg tccggagcag atcaaagagg gggccttcgc
     3901 cggtcagcgt cgtttccctt cgtgtacatt ccatagacaa gataccataa aaaccgcccc
     3961 gtgaacgtaa aaaagatatg gaaaaaagat tttcaagact ttcgggaagc gttccgaaac
     4021 gatctttgac ctctttttcg aggtcgtgca gctcatccag gctcccgacg cgcaacaacc
     4081 tcctgtagag cgcgatcctg atgctctcct ggggaatata aaacgaaggc acaaggcagg
     4141 gtatttccgc gctcaccgtc gcggcggagg ggacgactcc cctgatcttg ccgatctcct
     4201 cttcaagaag agtgcagtac agatgcgaat cggcaattcc gcgcccgctt ccgtgctggg
     4261 atgttcccag cagatcgccg ctgccgcgga tgcgcagatc ctgcagcgcc aaatcgtacc
     4321 cggcgccctc gtcgttgagc gacgtaatcg cgtcgaggcg ttccagcgtc tcagcggcaa
     4381 gcggccgtcc ctccgggtag agaaagtagg cgtatgcggc ctcttctctg cggccgacgc
     4441 gccccctgag ctggtacatc tgcgcgagcc cgagctcctg gcagtcttcg atgacgatcg
     4501 tgttcgcgcc ggggatatcg agtccgctct cgataatcgt cgtacacagg agaatctgaa
     4561 ttttcccgtt gtaaaaaccc atcatggtct cttcaagttc cgcctctttc attctgccgt
     4621 gcgccatgcc gatggcaacg tccggaaaga gacgacgcag gaatgccagt cgtttcggca
     4681 tccgcgtaat ccggttggtg acgtagtaga cctgtcctcc cctttcgagc tccctggaaa
     4741 ccgctccctt cacgatcgag gcgctccatg gaccggcggt ggtgactacg ggaatccggt
     4801 tgtgcggggc ctcgttgagc acggagatgc tcctgaggcc cttgagcgaa agagcgagcg
     4861 tccggggaat gggggtggcg gagagcatga gcacgtcgac gttctccctg gcctgtttca
     4921 gcttttcctt ggacatgacg ccgaaacgat gctcttcgtc gatgatgagc agcccgaggt
     4981 ctttgaacgc gacatcctcc ctgagcatac gcaccgtccc tatgaggatg tccacctcgc
     5041 cggcggcgac gcgctcgagc acgtccttct gctccttgcg cgagagaaaa cgcgagagaa
     5101 aggcaacccg gacaggaaaa ccggtcaagc gggcagtaaa cgagagatag tgctgctgcg
     5161 ccagtatggt ggtgggcacg aggagcgccg cctgcttccc gccctggact gcgcggaagg
     5221 ccgcccgcag ggcgacctcg gtctttccgt atccgacgtc gccgacgagc agtctgtcca
     5281 tgggaaagcg cctctccata tcctccagaa tctcggagat ggccacgagc tggtcttttg
     5341 t
//
LOCUS       DAOSWW010000051         5328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10303_length_5328_cov_4.154182, whole genome shotgun sequence.
ACCESSION   DAOSWW010000051 DAOSWW010000000
VERSION     DAOSWW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10303_length_5328_cov_4.154182"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PK442_01880"
     CDS             <1..954
                     /locus_tag="PK442_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOO86426.1"
                     /translation="YYRAAEYYGDPSASETAQTGMKCRDVFLKGMALASWKTEGVLIP
                     FGKENDFLPGYFLTPAKGSGKRKTLIAQSGFDGTAEEMYFAVGKAALERGYNVLLFEG
                     PGQTGKRRFHPDSSFVPDCAPALRAVVDFALARPEVHPAKIALYGASFGGYFALSGAV
                     GEPRLGAVIANSPLIDAQEHFFAGMGPHFVSMFQNQDLTAEEIRAMPEEELPAKYKFS
                     LLSLCVRYGKPSVKETLDVMRDFRLREDQLRSLNIPALGLVSEAEGTVPLAQAKRFAE
                     LAQKAALHIFTEESGANIHCQLDNMPLSWAVALDWLDEQFR"
     gene            complement(1061..1927)
                     /gene="lsrF"
                     /locus_tag="PK442_01885"
     CDS             complement(1061..1927)
                     /gene="lsrF"
                     /locus_tag="PK442_01885"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HOO86427.1"
                     /translation="MFEHMSWGKKNRLSSIVAPDGRALMLAIDHGYFMGATHGMEKPA
                     EAIEKLLPHVDSLMLSPGILSTCIDPALTQGVVLRASGGNSILDPDIDDEELILTAAQ
                     AVRMNASAVAVSIFVGSEHGHQTILNLTDMISQAAEFDLPVLGVTGVGKALKDRKEKR
                     YLSHAARLCAEMGADIVKTYYCEGFAEVVAKTPAPIVVAGGPKLDTYRDVIELTYKAM
                     QEGAIGVDMGRNVWQSDYPTAILQGVKAVIHGNASVKDAMEIVGELSTPENARKEYFK
                     VTAEDIANSAVH"
     gene            2133..3125
                     /locus_tag="PK442_01890"
     CDS             2133..3125
                     /locus_tag="PK442_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOO86428.1"
                     /translation="MQERPRDENSPRRQYLAEVAHLYYNQGKTQQEIAQLVGITRSGV
                     SRLLTEAREKGIVEILVHYPWQTSKALEDELSELFGLKDVRVLVRGGKNDREMLDGLG
                     SVAAQFFLNILRDGHIVGVSWGTALYSMLQAIRPVHRPDVKVVQLIGGTGAEHSSIVG
                     PLLAPMLANLLGCSCHYLHAPLITESEIFRKAVLQERPIRETLELGRKANIALVGIGA
                     THPEIYNPFRLGYISKDELNEISGNGAVGLICGRHYSIDGTELDIQVNRKVIGITLKE
                     LEEVEWVIGVAGGALKAESILGALRGRHVNVLVTDDIAARKALEIHKAGGSRAV"
     gene            complement(3129..4298)
                     /locus_tag="PK442_01895"
     CDS             complement(3129..4298)
                     /locus_tag="PK442_01895"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO86429.1"
                     /translation="MAMVESSVYRIPAVDLITFCGVVAEDVLSPHGALLLSRDADFAA
                     LSPSKRRGFAFSLLNAGVSSVAVRERPELSLNEILEVMARGKRPPEQRFRNLYEDAMY
                     YLTSFCEKVFSGGPGTLQSFTLLRAGRMLAEAVAADPDIPLHLGFVLRKDNLLLSHSI
                     NVGLLSAFLAHRMFPDREMTAVSVAAGGLLHDLGKTYISEEILNKPGALSEKEYETIR
                     RHPLVGEAALRKSGVTDSVMLKMVRNHHERMDGTGYPDGLSGESIPIAARIAAVADVF
                     DAVTSERAYKKRMSGRKGVSLLMDSIGTHLDSEVVRVFVGAFGMYPPGTEVVLSDGRS
                     GVVVAPGERGVLRPRVLLHRDERGDELSSAVFLDLAAAPALFIRRCAGEDVPEAV"
     gene            4675..>5328
                     /locus_tag="PK442_01900"
     CDS             4675..>5328
                     /locus_tag="PK442_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOO86430.1"
                     /translation="MRVAVLVKQVPATENVKIDETTGTMIREGVEAELNPLDLHAVEE
                     AVRLKERFEGTEVVVISMGPPQAKKAVKAAIAMGCDRGVLLSDRKFAGSDTLATARTL
                     AAGLVHEGGFDLALSGERATDGETGQVGPAVGRILDMSALTFVSEVESVGEGTIVVRR
                     AVEGGHERVEAPLPAMLSVVKEINVPRLCTLGGKIRAKKAEIPVVGAAELAIPEEHLG
                     "
BASE COUNT         1064 a         1619 c         1647 g          998 t
ORIGIN      
        1 tactacaggg ccgcggagta ctacggagac ccgtcggctt cagagaccgc gcagacggga
       61 atgaagtgcc gcgacgtctt cctcaagggg atggcgctcg cgtcatggaa gacggagggc
      121 gtgctcattc cgttcggcaa ggaaaacgat tttctgcccg gctacttcct cactccggcg
      181 aagggaagcg gcaagcggaa gacgttgatc gcgcagagcg gcttcgacgg cacggcggag
      241 gagatgtact tcgccgtcgg aaaggcggcg cttgaacggg ggtacaacgt gctcctcttc
      301 gagggaccgg gacagacggg gaagcggcgt ttccaccccg actcgtcctt cgtccccgat
      361 tgcgcccccg cattgcgggc cgtcgtggac ttcgcgctcg cccgccccga agtacatccg
      421 gcaaagattg cgctctacgg ggccagtttc ggcggctact tcgccctctc cggcgcggtc
      481 ggcgaacccc ggctgggcgc ggtgatcgcg aactcgccgc tcatcgacgc gcaggaacat
      541 ttcttcgccg gcatgggacc gcattttgtt tccatgtttc agaatcagga tctcacggcg
      601 gaggaaatac gcgccatgcc ggaggaggag ctgcccgcga aatacaaatt ctccctgctg
      661 agcctctgcg tgcgctacgg caaaccttcg gtgaaggaga cgctcgacgt catgcgggac
      721 ttccggctgc gcgaagacca gctccgctcg ctgaacatac ccgcgctggg actcgtcagc
      781 gaagcggagg gaacagtccc ccttgcgcag gcgaagcgat tcgcggagct ggctcaaaag
      841 gccgcgctcc acatcttcac ggaggagagc ggcgcgaaca tccactgcca gctcgacaac
      901 atgcccctct cgtgggcggt tgcgctcgac tggctggacg agcagttccg gtaggcacaa
      961 caacaaagga acggggcgag gaacgccgaa aagcgccccg ccccgttctt ttgttttgaa
     1021 tttatcccct tgcgactaca gtacagggag gaaaaacttt ctaatgcacc gcgctgttcg
     1081 cgatgtcttc ggccgtgacc ttgaagtact ccttccgcgc gttttccggc gtggaaagtt
     1141 cgcccacgat ctccatggcg tccttcaccg acgcattgcc gtggatgacg gccttcacgc
     1201 cctgcagaat cgccgtcggg tagtccgact gccacacgtt gcggcccatg tcgacaccga
     1261 tggcgccttc ctgcatcgcc ttgtaggtga gctcgatcac gtcgcggtag gtatccagct
     1321 tcgggcctcc ggcgaccacg atcggggcgg gcgtcttcgc cacaacctcc gcaaacccct
     1381 cgcagtagta ggtcttgacg atatcggcgc ccatctccgc gcacagcctt gcggcgtgcg
     1441 agaggtagcg tttctccttg cggtccttca gcgccttccc cacgcccgtc acgccgagca
     1501 ccgggagatc gaactccgct gcctgggaga tcatgtcggt gaggttcagg atcgtctgat
     1561 ggccgtgctc ggagccgacg aagatggaga cggcgaccgc cgaggcgttc atgcggacgg
     1621 cctgcgcagc ggtgaggatc agctcctcgt cgtcgatgtc ggggtcgaga atgctgttgc
     1681 cgccgcttgc gcgcaagacc acgccctgcg tcagggcggg atcgatgcag gtggagagaa
     1741 ttcccgggct caacatcagg gaatcgacgt gcgggagcag cttctctatc gcctccgccg
     1801 gcttctccat gccgtgcgtg gcgcccatga aatagccgtg gtcgatggcg agcatcagcg
     1861 ctctgccgtc gggagccacg atggaactca gtctgttctt ctttccccag ctcatatgct
     1921 caaacatagc gttcctcctt gtgacaaaaa gatttcacgt atgtgaaatt taatcacgaa
     1981 agtgatatac tctgcatgtc attctaacgt atgtccctgt attgtcaaga aaaaaactac
     2041 aaatttcttg agacgtcttt tcacttttat tctgctctat tctggtacaa tgcagcgcta
     2101 tacacggaga gacatccgag gaggggaaga gcatgcagga acgcccgagg gacgagaaca
     2161 gcccgagaag gcagtatctc gccgaggtgg ctcatctgta ctataaccag gggaagacgc
     2221 agcaggagat cgcgcagctc gtgggcatca cgcggtcggg ggtttcgcgg ctgttgacgg
     2281 aggcacgcga gaaggggatc gtcgagatcc tcgtccacta tccgtggcag acttcgaaag
     2341 cgcttgagga cgagctttcg gagcttttcg ggctgaagga cgtccgcgtc ctcgtccggg
     2401 gagggaagaa cgaccgcgag atgctcgacg gactgggttc cgtcgcggcg cagttcttcc
     2461 tgaacattct gcgggacggc cacatcgtcg gcgtctcgtg gggaacggcc ctctacagca
     2521 tgctccaggc gatccgcccc gtgcaccggc cggacgtcaa ggtggtgcag ctcatcggcg
     2581 gcacgggggc cgaacactcg tccatcgtcg gcccgctgct cgcgccgatg ctggcgaacc
     2641 tcctcggctg ttcgtgccac taccttcacg cgccgctgat cacggagagc gagatatttc
     2701 gcaaggcggt ccttcaggaa cgcccgatcc gcgagacgct cgaactcgga cgaaaagcaa
     2761 acatcgccct ggtgggcata ggcgcgacgc acccggagat ctacaacccc ttccgcctgg
     2821 ggtacatttc caaggacgag ctgaacgaga tctccggcaa cggcgcggtc gggctcatct
     2881 gcggccgtca ctactccatc gacggcacgg agctggatat tcaggtgaac agaaaagtga
     2941 tcggcattac gctgaaggag ctggaagagg ttgagtgggt gatcggcgtc gcggggggcg
     3001 cgctcaaggc ggagtcgatc ctgggagcgc tcagggggcg gcacgtgaac gtcctcgtca
     3061 ccgacgacat cgccgcgcga aaggcgcttg agatccacaa ggccggggga tcgcgggcag
     3121 tttaaggatc agacggcctc gggaacgtcc tctccggcgc atcttcgtat aaagagcgcg
     3181 ggcgcggccg caaggtcgag aaagacggcg gaagagagct cgtctccgcg ctcgtcgcga
     3241 tgcaaaagca ccctggggcg cagcaccccc cgttctccgg gggcgacgac gactccggac
     3301 cgtccgtcgg agaggacgac ctcggtgccg ggcgggtaca tcccgaaagc gccgacgaac
     3361 accctcacca cctcgctgtc gagatgcgtt cctatgctgt ccatcagcag cgaaacgccc
     3421 ttccttccgg acatgcgttt cttgtaggcg cgctccgacg tcaccgcgtc aaagacgtcg
     3481 gccacggctg caatcctcgc cgctatcggg atggactcgc ctgaaagccc gtccggatac
     3541 cccgttccgt ccatccgttc atgatggttc cgcaccattt tcaacataac gctgtccgtc
     3601 acgcccgact tccgcagcgc agcctccccc acgagcgggt gtctgcgtat tgtttcgtac
     3661 tctttttccg aaagagcccc cggcttgttc agaatttcct cggagatata cgtcttgcca
     3721 aggtcgtgca acaatccgcc cgcggcgacg gaaacagccg tcatctccct gtcggggaac
     3781 atcctgtgcg ccagaaacgc cgagagcagc cccacgttga tcgagtgcga gagcagcaga
     3841 ttgtccttcc tgagcacgaa tcccaggtgc agcggaatat cgggatcggc ggcgacggct
     3901 tcggcaagca ttcgccccgc acgaagcagc gtgaagctct gcagcgtgcc cggtccgccg
     3961 gagaacacct tttcgcaaaa ggaggtcaga tagtacatcg catcctcgta caaattgcga
     4021 aagcgctgct ccggggggcg tttcccgcgc gccataacct ccagaatttc gttcagcgag
     4081 agctccggcc gttcgcgtac cgcgaccgaa gaaaccccgg cgttcaggag ggaaaaagcg
     4141 aatcctctcc gcttggaagg agaaagcgcc gcgaagtcgg cgtcccttga gagaagcagc
     4201 gcgccgtgcg gggagagcac atcttcggca acaacgccgc aaaaggtgat gagatcgact
     4261 gccggaatcc ggtatacact gctttccacc atcgccatgc cctcctgtga gcgtgagatg
     4321 caaatcgaaa gaaaaggact ataccccatg cacgatgcat agagagagag ccgtttgagt
     4381 ataccatgct gcgactgccc tccccgcgct gcgagcaaca ggaacagtgc tccttgcgct
     4441 tcatttccgc aaagcggcgc gcctttttct cagtcgaaag actcccatga acaccaggta
     4501 cccggaagca tttgcgcctt catacccctt acaaaagaag tacctttgat aaataagtcc
     4561 tgttttatat gtatttttta tcattatata aataatagaa tcgtcttgct ttatgaaaat
     4621 ttagtggtag gatatcttct tatttcatgt tcattttttc gggaggtgcg tcgggtgcgc
     4681 gttgctgttc ttgtgaaaca ggtccccgcg acggaaaacg tcaagatcga cgagacgacg
     4741 ggaaccatga tacgcgaggg tgtggaggcc gagctgaatc cgctcgatct gcacgccgtg
     4801 gaggaggcgg tccggctcaa ggagcgtttc gaggggaccg aggtcgtcgt gatctccatg
     4861 gggccgcccc aggcaaaaaa agcggtgaaa gccgccatcg cgatggggtg cgacaggggc
     4921 gtgctgctct ccgacagaaa gttcgccggt tcggacacgc tggccacggc gcggacgctc
     4981 gccgcggggc tcgtccacga gggcgggttc gacctggcgc tctccggcga acgggcaacc
     5041 gacggcgaga cggggcaggt cggcccggcg gtcgggcgca ttctggacat gtccgcgctc
     5101 accttcgtca gcgaggtgga gtcggtcggc gagggcacga tcgtggtccg ccgcgcggtc
     5161 gagggagggc acgaacgcgt ggaggcccct ctccccgcaa tgctctccgt ggtgaaggag
     5221 atcaacgtcc cgaggctctg cacgctcggc gggaagatcc gcgcgaagaa ggcggagatc
     5281 cccgtcgtcg gggcagccga acttgcgatt ccggaggagc acctcggc
//
LOCUS       DAOSWW010000052         5309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10348_length_5309_cov_4.581271, whole genome shotgun sequence.
ACCESSION   DAOSWW010000052 DAOSWW010000000
VERSION     DAOSWW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10348_length_5309_cov_4.581271"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PK442_01905"
     CDS             complement(<1..1022)
                     /locus_tag="PK442_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO86431.1"
                     /translation="MTDKTYDIIIVGAGIVGATLARELSAYALEIAVIDKAADIPSGA
                     SRANSAMIHAGYDDKPGSIKADFCAPGNRLYQELAGPLDITLRKCGSYVCGFDAKDLK
                     HLEKLLDQGKRNGVPGVEIITGDALRAKEPHVSAEIAHALWAPSGCIVNNFEAVLAFM
                     DNAQQNGVELFLETELQDIVLSDDRKEVLGIRTNRGFMRTSVVINAAGINSDLVAKMA
                     GDDSFSIEPTRGEYYIMDKNVGNLVGSFFFPCPTEKGKGITVAPTAENNLLIGPTSVV
                     QKSRDDTSTSAEGLKEVVDGARLLVPAIPLGMAITTFSGLRASSSTKDFVLGVLDTPR
                     GFVNVAG"
     gene            complement(1171..2682)
                     /gene="glpK"
                     /locus_tag="PK442_01910"
     CDS             complement(1171..2682)
                     /gene="glpK"
                     /locus_tag="PK442_01910"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOO86432.1"
                     /translation="MSEKKYVVAIDQGTTSSRAIVFDLEGTPKNSHQMEHEQIYPKPG
                     WVEHNPMEIWSRVQDVIREVLNTNGVKADEIGAIGITNQRETTVVWEKATGKPVYNAI
                     VWQCMRTQDFCAEWQKKPGWDKKVNDLTGLVISPYFSGTKIKWILDNVPGVRAKAEKG
                     EVLFGNIDTWVTWNLTGGPNGGVHVTDVTNASRTLLMNIETLKWDKEMMDFLGVPEAM
                     LPAIKPSSYVYGYTPESGPFGAKIPVSGILGDQQAALFGQTCYSPGEAKNTYGTGCFM
                     LLNIGDKPVRSKNGLLTTVAYGLEEGKATYALEGSIAITGAAVQWLRDNLRLFDDAPD
                     SEYFAAKVKDSGGIYFVPAFSGLYAPYWDMSARGAILGLTRYITKEHIIRATLESICY
                     QTRDVQAAMDADSGVPLKALKVDGGAVKNNLLMQMQADILGVPVVRPVVNETTALGAA
                     YAAGLATGLWKSTEELRKHWKVDKQFDAAMPADKREEMYKGWKKAIDKSKGWL"
     gene            complement(2845..3243)
                     /gene="dhaM"
                     /locus_tag="PK442_01915"
     CDS             complement(2845..3243)
                     /gene="dhaM"
                     /locus_tag="PK442_01915"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HOO86433.1"
                     /translation="MNAILVVSHSAEAARGIVAIALQMAGERVGIAACGGTDDGELGT
                     SVPSILSALEGLLETSDGVLVIPDLGSAVLAARTAKDLLGERAGRVLIADGPVLEGTL
                     MASVEASVGAGLDRLAAVVDETRGLRKLQE"
     gene            complement(3244..3882)
                     /gene="dhaL"
                     /locus_tag="PK442_01920"
     CDS             complement(3244..3882)
                     /gene="dhaL"
                     /locus_tag="PK442_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HOO86434.1"
                     /translation="MNLDVQGMLKVFDAIGETMAAHKEYLTELDSAIGDADHGINMNR
                     GFHKVHDKLSAGSYTDLGALFKDVGMTLMGNVGGASGPLYGTFFMRMSTKLAGRQDVD
                     TVSLADAMEEGLKGIVALGKAQLQDKTMVDALTPAVEALKKASDNGVDLCGALEAGVA
                     AAQSGMESTIPLVARKGRASYLGERSAGHQDPGATSIYLILSTILRSVKESS"
     gene            complement(3893..4891)
                     /gene="dhaK"
                     /locus_tag="PK442_01925"
     CDS             complement(3893..4891)
                     /gene="dhaK"
                     /locus_tag="PK442_01925"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOO86435.1"
                     /translation="MKKLLNKVDDVVVESLAGLAAAHPGLVKLHPSVKAVLRADAPQK
                     KVAILSGGGSGHEPLHGGYVGYGMLDAACPGEVFTSPTPDQMYEAAKAVDGGKGVLFI
                     VKNYTGDVMNFQMAADLLAADGIPVAQVVIDDDVAVQDSLYTAGRRGVGGTVLIEKIV
                     GAKAEAGASLEEVKALCEKVNSQVRSMGMALTSCAVPAAGKPTFELGEDEMEIGIGIH
                     GEPGRERMKLKTAKEIVEMMAKAIVDDLPFKAGDEVVAFVNGMGGTPQMELYLAYNDL
                     VNFLKGKGIVIARSLVGNYITSLEMQGFSITLLKLDGEMKALWDAPVETAGLRWGR"
     gene            complement(4997..>5309)
                     /locus_tag="PK442_01930"
     CDS             complement(4997..>5309)
                     /locus_tag="PK442_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HOO86436.1"
                     /translation="TIFLVVPIMFIFSSGVKEALGGLTPGFGPFLVGWLVMVIGMALG
                     GPTGYAINPARDLGPRIAHFVLPIAGKGDSDWGYAWIPVLAPLTGGAVSYFIAHALGV
                     M"
BASE COUNT          978 a         1595 c         1624 g         1112 t
ORIGIN      
        1 cccgcgacgt tgacaaagcc tcgcggagta tcgagcacgc cgaggacaaa gtctttcgtg
       61 ctcgagctcg cccggagccc ggagaaggtc gtaatggcca tgccgagcgg gatggcggga
      121 acgagaaggc gggctccgtc gacgacctct ttgagccctt cggccgacgt ggaagtatcg
      181 tcccggctct tctgcaccac ggaggtaggg cctataagga ggttgttctc cgccgtcggg
      241 gccacggtga tcccctttcc tttttcggtc gggcatggga agaagaagct gccgacgagg
      301 ttgcctacgt tcttgtccat gatgtagtac tcgcctctcg tcggttcgat agagaatgaa
      361 tcgtcgccgg ccatcttcgc gacaaggtcg gagttgatcc cggccgcgtt gatgacgacg
      421 gaggtacgca taaagccgcg gttggtgcgt attccaagaa cctctttccg gtcgtccgag
      481 aggacgatgt cctgaagctc tgtttcgagg aagagctcga cgccgttctg ctgcgcattg
      541 tccataaacg cgaggaccgc ttcaaagttg ttcacgatgc atccggaggg cgcccacagc
      601 gcgtgggcga tctccgcgct gacatgcggc tccttggcgc gcagggcatc gccggtgatg
      661 atctccactc cggggacgcc gttgcgcttt ccctggtcga gaagtttttc aaggtgcttc
      721 aaatctttgg catcgaagcc gcagacgtag gaaccacact tccggagcgt tatatccagc
      781 ggaccggcca gttcctgata gagtctgttg ccgggggcgc aaaaatccgc cttgatggag
      841 cccggcttgt cgtcgtatcc ggcgtgaatc atggcgctgt tggccctgct cgccccggag
      901 ggaatatccg ccgccttgtc gatgacagcg atttcaaggg cataggcgga aagctccctg
      961 gccagggtag ccccgacgat ccctgcaccg acgatgatga tatcgtaggt tttgtccgtc
     1021 aacttcagaa cacctccata gtacgaagag ggagaaaggt gcgtgccatg ggcagcgtac
     1081 ggcctctctc cctctgtcgc gcagtgcgat ttatttcaga agacttcaca aaatcggccg
     1141 ctgcgtatgt ccgcagacag cgtcaggtct ttagagccac cccttggact tgtcgatggc
     1201 tttcttccac cccttgtaca tctcttcgcg cttgtctgcg ggcattgcgg cgtcgaactg
     1261 cttatcgacc ttccagtgct tgcgcagctc ctcggtgctc ttccagagac cggtggcaag
     1321 tcctgcggcg tatgctgcgc cgagcgccgt ggtctcgttg acgaccgggc ggacgacggg
     1381 cacgccgagg atgtcggcct gcatctgcat gaggaggttg ttcttgactg cgccgccgtc
     1441 gaccttcagg gccttcagag gtacgccgga gtcggcgtcc atagccgcct ggacgtcgcg
     1501 ggtctggtag cagatgctct ccagcgtcgc gcggataatg tgctccttgg tgatgtagcg
     1561 ggtaaggccg aggatggcgc cgcgggcgct catatcccag tagggggcgt agagcccgga
     1621 gaacgcgggg acgaagtaga tgccgccgct gtccttcacc ttcgctgcga agtactcgga
     1681 atcgggggcg tcgtcgaaga ggcgcagatt gtcgcgcagc cactggactg cggcgccggt
     1741 gatggcgatg gatccctcaa gagcgtaggt cgccttgccc tcttcaaggc cgtaggcaac
     1801 cgtcgtcagc agaccgttct tggagcggac cggcttgtcg ccgatgttca gcagcatgaa
     1861 gcagccggtg ccgtaggtgt tcttcgcttc gccggggctg tagcatgtct ggccgaagag
     1921 cgccgcctgc tggtcgccga ggatgccgga tacggggatc ttcgcgccga aggggccgct
     1981 ctcgggggta tagccataga cgtagctgct gggcttgatc gcggggagca tcgcctcggg
     2041 aacgccgagg aagtccatca tttccttgtc ccacttcagc gtctcgatgt tcatgaggag
     2101 ggtacgggag gcgttggtga cgtcggtgac gtggacgccg ccgttggggc cgccggtgag
     2161 gttccaggta acccatgtgt cgatgttgcc gaagagaacc tcgccctttt ccgccttcgc
     2221 gcggacgccg gggacgttgt cgagaatcca cttgatcttc gttccggaga aataggggct
     2281 gatgacgagg ccggtcagat cgttgacctt cttgtcccag ccgggcttct tctgccattc
     2341 ggcgcagaag tcctgcgtcc tcatgcactg ccagacgatt gcgttgtaga ccggcttgcc
     2401 ggtggccttc tcccagacga cagtggtttc gcgctggttc gtgatgccga tggcgccgat
     2461 ctcgtccgcc ttgactccgt tcgtgttcag tacctcgcgg atgacgtcct gcacgcggga
     2521 ccagatctcc atcggattgt gctccaccca gcccggcttc ggatagatct gctcgtgctc
     2581 catctggtgc gaattcttcg gagttccctc aaggtcgaaa acaatggctc ggctgctggt
     2641 cgttccctgg tctatggcca caacgtattt cttctcggac atgacagtac ctcctgtttg
     2701 gggtttatcg aggcgtgaga acaagttgct gaaccagttg ggcatgatcg taccgcctcc
     2761 tttatttgcg atgccgccga gacgtgaacg ccactatagc gcctcccggc tgtttcgcgt
     2821 catacacgag ctatgaacag agccttattc ctgtagcttt ctcaatcccc tggtttcgtc
     2881 cacgacggcg gcgagccggt caagccccgc cccgacgctg gcctccaccg aggccatgag
     2941 ggttccctcc agaacagggc cgtcggcgat gagtacgcgg cctgcacgtt ctccaaggag
     3001 atccttggcc gtgcgcgccg caagaacagc gctccccagg tcggggatga cgagaacgcc
     3061 atcggatgtt tcgagcagcc cttcaagcgc agagagaatc gacggaaccg aggttccgag
     3121 ttcgccgtcg tcggttccgc cgcatgcggc gataccgacc cgctcccccg ccatctgcaa
     3181 ggcgattgcc acaatgcccc gcgcggcttc ggcgctgtgg gaaacgacga gaatggcgtt
     3241 cactcaggaa ctctccttta cggagcgcag aatcgtcgag agaatcaggt agatggacgt
     3301 cgctccggga tcctggtgtc ccgccgaacg ctcgccgagg tagcttgccc gccctttgcg
     3361 ggcgacaagg ggaattgtcg actccatacc cgactgcgcc gcagcgacgc ccgcttcgag
     3421 agcgccgcaa agatcaacgc cgttgtcgga agcttttttc aaagcctcga ctgccggcgt
     3481 cagcgcgtcg accatggtct tatcctgaag ctgggctttg ccaagggcaa cgatgccctt
     3541 gagcccctct tccattgcat cggccaggga gacggtatcc acatcctgcc ttccggcgag
     3601 tttcgtcgac atgcgcatga aaaacgttcc gtagagaggt cccgaagcgc cgccgacatt
     3661 tcccatcagg gtcatgccga catccttgaa aagagcgccg agatctgtgt aggatcctgc
     3721 ggagagcttg tcgtgaacct tgtgaaaacc gcggttcatg ttgattccat ggtcggcgtc
     3781 gcctatagcc gaatcgagtt cggtcaggta ttccttgtgc gccgccatcg tctcgccgat
     3841 agcgtcgaac accttgagca ttccctggac atcgaggttc atctctgcgc gcctacctcc
     3901 cccagcgaag ccccgccgtc tcgacgggag cgtcccaaag ggccttcatt tctccgtcga
     3961 gtttcagcaa ggtaatggag aaaccctgca tttcgaggct tgtgatatag ttgccgacga
     4021 gagaacgtgc aatgacgatc cccttcccct tgaggaaatt gacaagatcg ttatatgcaa
     4081 ggtagagttc catctgcggc gtgccgccca tgccgttgac aaaggcgaca acttcgtcgc
     4141 ccgctttaaa tggaagatcg tccacgatgg cctttgccat catctccaca atctccttcg
     4201 ccgttttcag cttcatgcgc tcacggccgg gctcgccgtg aatgccgatg cctatttcca
     4261 tctcgtcctc gccgagctcg aaggtcggtt tgcccgcagc cggaacggcg cacgatgtga
     4321 gcgccatgcc catggagcga acctgactgt tgaccttttc gcagagcgcc ttaacctctt
     4381 cgagcgaagc gcccgcttcc gccttggcgc cgacgatctt ttcgatcaga acagtgccgc
     4441 cgactccgcg gcgtcccgcc gtatacagcg aatcctgaac ggcgacgtcg tcgtcgatga
     4501 cgacctgcgc gacgggaatg ccgtccgcag caagaagatc ggcggccatc tggaagttca
     4561 tgacgtcgcc cgtgtaattc ttcacgataa agaggacgcc cttaccgccg tccacggcct
     4621 tggccgcctc gtacatctga tcgggagtgg gagaggtgaa cacttcgccg gggcaggccg
     4681 cgtcgagcat accgtacccc acgtaccctc cgtgaagagg ttcgtgcccg ctgccgccgc
     4741 cggagaggat ggcaaccttt ttttggggcg cgtcggccct caggacagcc ttgaccgacg
     4801 ggtggagctt caccagaccg ggatgcgccg cggcgagacc ggcgagcgat tcgacgacga
     4861 cgtcatccac tttgttgagg agctttttca ctaaacaacg cctcctttca aggagcaggt
     4921 tctattccgg agaaccggcg gaggaggcgt ttgtgtttct cccccgccgg cctaatttcg
     4981 aaaagaaaac cgtgcgttac atcacgccga gagcgtgtgc gatgaagtag gagactgcgc
     5041 cgccggtaag aggcgcaagc acggggatcc aggcatatcc ccagtccgaa tcgccttttc
     5101 cggcgatcgg gagtacaaag tgcgcaatcc tggggccgag gtcgcgggcc gggttgatgg
     5161 cgtatccggt cgggccgccg agtgccatac cgataaccat gacgagccag ccgacgagga
     5221 agggaccgaa accgggggta aggccgccga gggcttcctt gacgccggag gagaagatga
     5281 acatgattgg aacaacgagg aaaatggta
//
LOCUS       DAOSWW010000053         5271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10459_length_5271_cov_3.481787, whole genome shotgun sequence.
ACCESSION   DAOSWW010000053 DAOSWW010000000
VERSION     DAOSWW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10459_length_5271_cov_3.481787"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..338)
                     /locus_tag="PK442_01935"
     CDS             complement(123..338)
                     /locus_tag="PK442_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86437.1"
                     /translation="MVEQKKKIQKAQQTSLFAKQLRIVFYTVFICITIVLLAVTLDLV
                     INPAKTWSDSLAIRWISEKRAMFPFFK"
     gene            complement(601..>2312)
                     /locus_tag="PK442_01940"
     CDS             complement(601..>2312)
                     /locus_tag="PK442_01940"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HOO86438.1"
                     /translation="PDGHVSVEDNGRGIPTDPHPSNGRPASEVVLTTLHAGGKFDGGA
                     YKVSGGLHGVGVSVVNALSDWLEITICRNGESRSQRFEKGIPATDLSEGIPTEHTGTK
                     VHFLADATIFEEVKYSFDVLGARFREMAFLNPGLSITLDDKREGGRVKEYRYEGGMRS
                     FIEYLNRGKTVLFPDPIVISGEKDGVSVDIGLQYNDGYQERVFGFANLIHTIEGGTHV
                     SGLRTALTRAVNEAARRAKLLKEKEENLSGDDLKEGLTSVISVKLGNPQFEGQTKTKL
                     GNSDVKGIADSIVYEGLVAWFDDHPQIIKTVVEKAIKARQAREAAKRARELVRKTAMT
                     GLTLPGKLADCSGRNPENTEVYIVEGNSAGGSAKQGRDRSFQAILPLRGKILNVEKAR
                     LDKALANAEIRTMIQTLGAGVGDDFDTSKLRYHKVIIMTDADVDGAHIGTLLLTFFYR
                     YMPALIEKGYLYMAQPPLYRVQRGKNVTYCYNEKEMRALLDEAGESAKIVVQRYKGLG
                     EMNPEQLWETTMDPKNRILRKVEIDDEVEVDEYFSILMGDKVEPRRDFITAHAHEVRN
                     LDI"
     assembly_gap    2313..2322
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2323..2557)
                     /locus_tag="PK442_01945"
     CDS             complement(<2323..2557)
                     /locus_tag="PK442_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86439.1"
                     /translation="MITSPSALQYTAKDIQVLEGLQAVRKRPGMYIGDTSVRGLHHLV
                     YEVVDNSVDEAIGGFCTRIAVTIHENESISVEDN"
     gene            complement(2554..2775)
                     /locus_tag="PK442_01950"
     CDS             complement(2554..2775)
                     /locus_tag="PK442_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86440.1"
                     /translation="MFIPLEGDGLVSIRRIVALVRHENETAVYLRDGSLLATGFKPET
                     LRKRYNAFCKEARANAEPLRAQRNGGKRT"
     gene            2904..3806
                     /locus_tag="PK442_01955"
     CDS             2904..3806
                     /locus_tag="PK442_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOO86441.1"
                     /translation="MEIKRMRTLARRGAARVLSEIRDEWKAFLAVTIGVSIQAVAVVL
                     FVLPNRFPDLGVSGIAVLSNYVFGISPSWVILILNALLMIWAWRELSPRFVMWTMWAV
                     LLFSLLLKLFEFVPVPVIGDKFLAAMVSGVLRGLGAGLVFRVGGSTGGLDIPGVALRK
                     RYGIEMGQFSIYINMGLLGLSFFVVGLDAAIYGAVGLYVFGIVVDNTTRSFDRRKQVF
                     IITNIPEEVSAYINLSLHRGTTRFEGVGGYSKQKRPVLMTLLEPRQVIPLKRFLAEND
                     PKAFMSVSDASEVVGKGFKSWKSL"
     gene            complement(3885..5096)
                     /locus_tag="PK442_01960"
     CDS             complement(3885..5096)
                     /locus_tag="PK442_01960"
                     /inference="COORDINATES: protein motif:HMM:NF016100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO86442.1"
                     /translation="MTFDVHEISPLEWDDMADGFLDLVDNEIRAAEMLLNADPPLAMP
                     SIPHSLIATEYLLFLAVQLLGASKQSWTNVDEYIKFIAEKAPDLRRTLGPLTPLKKAA
                     AAGAFDEDIFKVAESAFKATVKAEKILGEFIDKKLEELYAEHETSPASPSGGSGGRGN
                     EYGQAMEILDKAWGETSVKKRATLVKKALALCPDIADAYVILGNDALDSGKPEEAIEH
                     YRRGLEAGERELGAEAFTDDKGHFWGLLETRPYMRARFGLASALHKSGSVREASEHYK
                     AMLELNPNDNQGVRYMLIEALLLLGEHQDADALLKQHDEESAFMLYPKALLEFVKNGD
                     SKVAQKLRAEAIRNNPHVPAFLLGKKKLPKKTPDSYGFKDESEAVVYALEFGAPWSTI
                     PGAKEWLAQGV"
BASE COUNT          902 a         1580 c         1581 g         1198 t
ORIGIN      
        1 gatattacgc tgctctgctt ccgctacacg ggagggaaca aaaaggtata ggagcgggaa
       61 ctgaaaggat ggcgcgcaca acggagggcg tccgaaaagg gcccctccgt tgtgtctgtg
      121 tattatttaa aaaaggggaa catcgcccgt ttttcgctga tccatcggat ggcaagagaa
      181 tccgaccatg ttttggcggg attgatgaca agatccagcg tcaccgcgag aagaacgatg
      241 gtaatgcaga taaagacggt atagaacacg atgcgaagct gttttgcgaa gagggatgtc
      301 tgctgcgctt tctggatctt cttcttctgt tcgaccatgc gcgatcatct cctttgtctc
      361 tctgttgcgt tctttgaatt atacacgttt tctttccgtt tgatcgggac ttttgccccg
      421 tacaggggaa gatgagagaa cggagcggaa cgcgacctcg gatgttccgc gaggaggaag
      481 agatttgcgg gagcatcgcg tggcgccgtg tgccggagag cgttggagcc gccccctgcg
      541 cggcgggtgc gaaaaagaaa aaccccggag cgacatctcc ggggcgttgt ccttcgcggg
      601 ctaaatgtcc aggttcctga cctcgtgcgc gtgggcggtg atgaagtccc tgcggggctc
      661 gaccttgtcc cccatcagga tgctgaaata ctcgtccacc tcgacctcgt cgtcgatctc
      721 gaccttcctg agaatgcggt tcttcgggtc catcgtggtc tcccagagct gttcggggtt
      781 catctctccc aagcctttgt agcgctgcac cacgatcttg gcggattcgc ccgcctcgtc
      841 cagaagcgcc cgcatctcct tctcgttgta gcagtaggtg acgtttttcc cccgctgcac
      901 cctgtagagc ggcggctgcg ccatgtacag gtaccccttt tcgatcagcg cgggcatgta
      961 cctgtagaag aaggtcagca gcagggtgcc gatgtgcgcg ccgtcgacgt cggcgtcggt
     1021 catgatgatg accttgtggt agcgcagctt cgacgtgtcg aagtcgtctc ccacgccggc
     1081 gccgagcgtc tggatcatcg tgcggatctc cgcgttggcc agagccttgt ccaggcgcgc
     1141 cttctcgacg ttgaggatct tgccgcgcag cggaaggatc gcctggaaac tcctgtcgcg
     1201 cccctgcttt gcgctgccgc cggcgctgtt tccctcaaca atgtacacct cggtgttctc
     1261 ggggtttctg ccggagcagt ccgccagctt accggggagc gtcagtcccg tcatcgcggt
     1321 cttgcggacc agttcgcggg cgcgttttgc cgcctcgcgg gcctgccgcg ccttgatggc
     1381 cttctcgacg acggtcttga tgatctgcgg atgatcgtcg aaccaggcga ccagcccctc
     1441 gtagacgatg gagtcggcga ttcccttcac gtcgctgttg cccagcttcg tcttcgtctg
     1501 cccctcgaac tgcgggttcc caagcttcac ggagatgacg gaggtcagtc cctccttgag
     1561 gtcgtcgccg gagaggtttt cttccttctc cttcagcagc ttggcccggc gagcggcttc
     1621 gttcactgcg cgggtgagcg ccgtccggag accggagacg tgcgtccccc cctcgatcgt
     1681 gtggatcagg ttggcgaagc cgaagacgcg ctcctggtag ccgtcgttgt actgaaggcc
     1741 gatgtccacg gaaacgccgt ccttctcgcc cgaaatcact atcggatcgg ggaagagcac
     1801 ggtcttgccg cggttgaggt actcgatgaa ggagcgcatt cccccttcgt agcggtactc
     1861 tttgacccgt ccgccctctc tcttgtcgtc gagcgtgatg gagagtcccg ggttcaaaaa
     1921 ggccatctcc cggaatcgag cgccgagcac gtcgaaggag tacttcacct cttcaaagat
     1981 cgtcgcgtcg gccaggaagt gcaccttggt cccggtgtgt tcggttggaa taccctcgga
     2041 gagatccgtc gcgggaatgc ccttctcgaa gcgctggctt cgcgactctc cgttgcggca
     2101 tatcgttatc tcaagccagt cggagagggc gttcactacc gaaacgccaa ccccgtggag
     2161 gccgccgctg accttgtatg cgccgccgtc gaacttgccg cctgcatgga gcgtggtgag
     2221 caccacttcc gaggccggac ggccgtttga aggatgaggg tccgtgggga tgccgcggcc
     2281 gttgtcctcg actgagacat gcccgtcggg gtnnnnnnnn nncgttgtcc tccaccgaga
     2341 tgctttcgtt ctcgtggatg gtcactgcga tccgggtaca gaagccgccg atcgcctcgt
     2401 ccaccgagtt gtccaccact tcgtagacca ggtgatggag cccccggacg gatgtgtccc
     2461 cgatgtacat tcccggccgc ttgcgaaccg cctgaagacc ttcaaggacc tggatatcct
     2521 tggcggtgta ctgaagagcc gaagggctcg ttatcatgtt ctctttcctc cgtttcgttg
     2581 cgcgcgaaga ggctcggcgt tcgctcttgc ctctttgcag aaagcgttgt atcttttcct
     2641 cagtgtttcg ggtttgaatc cggtggcgag gagtgagccg tcgcgcaaat agaccgccgt
     2701 ttcgttctca tgacgaacca gggcgactat gcggcgtatg gagacaagac cgtctccttc
     2761 gagggggatg aacatgttgt ttcactccat ttcgcaagtc tttaactgaa ctattatacc
     2821 agaaagccgc ggagagtgcg cggaaatgcg ctccgaatcc gttggagtcg gagaatttga
     2881 aagaagcaag aggagtggtc gcaatggaaa taaaaagaat gcgcacattg gcgcgcagag
     2941 gcgccgcgag agttttgagc gaaatacgcg acgagtggaa ggcgttcctc gccgtcacga
     3001 taggcgtctc cattcaggcg gtcgcggtgg tgctcttcgt gctgcccaac cgcttccccg
     3061 atcttggcgt ttcgggtatc gcggtgctgt cgaactacgt cttcggaatc tccccctcct
     3121 gggtgatact gatactgaac gccctgctga tgatctgggc gtggagggag ctttcgccgc
     3181 gcttcgtgat gtggaccatg tgggcggtgc tcctcttctc gctgctgctg aagctctttg
     3241 aattcgtccc cgttcccgtg atcggcgaca aattccttgc cgccatggtc tccggcgtgc
     3301 tgcgcggtct cggcgctggg ctcgtcttcc gcgtgggcgg ctccaccgga ggtctcgaca
     3361 tccccggcgt cgccctgcgg aagcgctacg gtatcgagat ggggcagttc agcatctaca
     3421 tcaacatggg gcttctcggg ctctccttct tcgtcgtggg cctggacgcg gccatttacg
     3481 gcgcggtggg gctgtacgtc ttcgggatcg tcgtcgacaa cacgacgcgc tcgttcgacc
     3541 gcagaaagca ggtcttcatc atcacgaaca tccccgagga agtttcggcg tacatcaacc
     3601 tctcgctgca ccgggggacg acccgtttcg agggtgtggg cgggtactcc aagcagaagc
     3661 gccccgtctt gatgactttg ctcgaaccgc gccaggtcat tccgctgaag cggtttctcg
     3721 ccgaaaacga ccccaaggcc tttatgtccg tctccgacgc ctcggaggtc gtagggaagg
     3781 ggtttaaaag ctggaagagt ctctagcgcc ggcaagaagc cataaaaaac cggagaccgg
     3841 atcacgggaa acgccgcgtc cggtctccgg tttttttgtg tccgctacac tccctgcgcc
     3901 agccactcct tggctccggg gattgtcgac caaggagcgc cgaactcaag cgcgtagacc
     3961 acggcctcgc tctcgtcttt gaaaccgtac gagtcgggcg ttttcttcgg cagcttcttt
     4021 ttcccgagga ggaacgccgg gacgtgcggg ttgttccgta tcgcctcggc gcgcagcttt
     4081 tgcgccacct tcgaatcgcc gttcttcaca aattccagga gggccttcgg gtagagcatg
     4141 aacgcgctct cttcgtcgtg ctgcttcagg agcgcgtccg cgtcctggtg ctctccgagg
     4201 agcagcagcg cttcgatgag catgtagcgc actccctggt tgtcgttcgg gttcagttcg
     4261 agcatcgcct tgtagtgttc cgaggcctcg cgtacgctgc cgcttttgtg cagcgccgag
     4321 gcaagaccga agcgggcgcg catgtagggg cgggtttcca gaaggcccca gaaatgccct
     4381 ttgtcgtcgg taaaggcttc cgcgcccagc tccctttccc cggcttccag ccccctgcgg
     4441 tagtgttcga tcgcttcttc cggcttcccg gaatcaagag cgtcgttgcc cagaatgaca
     4501 tacgcgtcgg cgatatcggg gcagagcgcg agcgcctttt tcaccagcgt tgcccgcttc
     4561 ttcaccgacg tctcccccca cgccttgtcg aggatttcca tcgcctgccc gtactcattc
     4621 ccgcggccgc cggaaccgcc ggagggagac gcgggcgagg tctcatgttc ggcgtacagc
     4681 tcctccagct tcttgtcgat gaattcgccg aggatctttt cggccttcac cgtcgccttg
     4741 aaggcggatt cggcgacctt gaagatgtcc tcgtcgaacg ctcccgccgc ggcggccttc
     4801 ttcagcgggg tcagcgggcc gagcgtacgg cgaagatcgg gcgccttctc cgcgatgaac
     4861 ttgatatact cgtcgacatt cgtccaggat tgcttcgagg cgcccaatag ctgtacggcc
     4921 agaaagagca ggtattccgt cgctatgagc gagtgaggta tcgagggcat ggccagtggc
     4981 ggatcagcgt tcaacagcat ctccgccgca cgtatttcgt tgtcgaccag gtcgaggaat
     5041 ccgtcggcca tatcgtccca ttcaagcggg cttatctcgt gtacatcaaa cgtcatctcg
     5101 atccatccct tccttccgtc ttcgactccc gagggaggat gtgcggttcg gcgcgcgccc
     5161 cgaatgccgg atcgtcccgg ggaaagtccg gacgcacgcg gcgaacgaag cggtttatcg
     5221 ttcacacatc cactcaatac taagcgtctc tttctctctc gtcaatttct t
//
LOCUS       DAOSWW010000054         5267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10470_length_5267_cov_4.425940, whole genome shotgun sequence.
ACCESSION   DAOSWW010000054 DAOSWW010000000
VERSION     DAOSWW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10470_length_5267_cov_4.425940"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1506)
                     /locus_tag="PK442_01965"
     CDS             complement(232..1506)
                     /locus_tag="PK442_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOO86443.1"
                     /translation="MADATPFLRGLRGATILGINPPVFDFTFFDLWAKPLGLLFLLDA
                     LRRRGNTVHLLDCIAEGSGEQLSFGRRKLLRREISKPAPFRNIPRRYHRFGLDDAEIL
                     LRLHALPRPDCIMVTSGMTYWYEGVFRAVELAKEVFPGVPVLLGGVYARLCPEHAGRS
                     GADIVQDILPDIPFVRPAMELYSEPGYALLLTSFGCPLSCEYCASKLLFPQFIERDLS
                     ETLADAAFQFSLDGVHDAAFYDDALLIDKERRFYPLCEELVRRFPGVRYHTPNGLHIR
                     EIDERCAAVLKQTGFHTLRLSLESSDPAFQRKGSGKTSGEEFQHALRNLQKAGFAPEQ
                     METYILAGLPGQTTVSVEASIRFVDSCGARAKLAEFSPVPGTPSFEEAASILPELRSE
                     PLLHNKTVYSSFLSGNISPEKLQFLKDLTKHR"
     regulatory      1678..1786
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1824..2339
                     /gene="thiT"
                     /locus_tag="PK442_01970"
     CDS             1824..2339
                     /gene="thiT"
                     /locus_tag="PK442_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOO86444.1"
                     /translation="MFHVRNTILVEGALAVALSVVFSALRLWTMPQGGSITLEMLPIF
                     LFALRRGGKAGCAAGAVSGVMQLITGGYIVHPAQALLDYPLAFGVLGVTGFFKNRPLW
                     LGITLGGVLRFFCHVLSGVVFFGSFAPEGTNVWMYSAVYNGSFMAPTLVVCGVLAYLI
                     WPRLRRVGAEG"
     gene            complement(2314..3396)
                     /locus_tag="PK442_01975"
     CDS             complement(2314..3396)
                     /locus_tag="PK442_01975"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOO86445.1"
                     /translation="MVHSSPNITLEEGAAPLLRWYDACRRPFPWRETNDPYAVWISEV
                     MLQQTQAARGVEYHRRWMRTFPTLRSLAEAKDDDVLKAWEGLGYYSRARNLLRAARLL
                     QARGLATLPASFEELRALPGIGPYTAAAVASIAFGLPVPALDANGKRVFSRLLDLEEP
                     VDRRAGEKSVLRAFVQMLPADRPGDFNSAIMELGATLCAPTSTLCGECPLEFLCLARL
                     RGSALERPVLSRQTKSESLEGILAVFVSGDRVFLRRRPPAGLWANFEEFPWSAPPAEQ
                     ESPLTELARRSNADCGGEVRCSFTKWRLRLAVRIVRLHAPGDLENMNGRWASPSELAA
                     ATLPAGSRKMRERLFGEGTIPPPPPS"
     gene            3589..>5267
                     /gene="glgB"
                     /locus_tag="PK442_01980"
     CDS             3589..>5267
                     /gene="glgB"
                     /locus_tag="PK442_01980"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOO86446.1"
                     /translation="MAKTENIRYDFSLFGEMDSYLFREGNHFRLYEKLGAHVVDDGKL
                     PGTYFALWAPNAREVSVIGAFNDWDRTRHYLSPRKDNSGIWEGFVPGVGKGALYKYSL
                     RSRNGSVFEKGDPFAAFWEIAPKTASIVWPCDYAWNDGEWMKNRRAQNALNAPMSVYE
                     VHVGSWRRKPAEGFRSLSYRELGHELGEYVRAAGFTHVELLPVMEHPFYGSWGYQTLG
                     YFAPTSRYGTPEDFMYMVDVLHQMGIGVILDWVPSHFPADAYGLAHFDGTCLYEHEDP
                     RKGYQPDWGSQIFNFGRNEVREFLISSAFCWLDLFHADGIRVDAVASMLYLDYSRKEG
                     EWIPNQFGGRENLEAVGFLKKMNEAIYQEFPDVQTIAEESTAWPMVTRPTFIGGLGFG
                     MKWNMGWMHDILEYMQKDPVYRKYNHNKITFSIMYAFTENFLLPFSHDEVVHGKGSML
                     GKMPGDEWQQYANLRLLLGYMYAHPGKKLLFMGSEFGQGREWNHDDSLFWDLLHSPRH
                     KGMLDWVTDLNRVYRSEAALHSRDFSPEGFQWCDFSDWEQSVVSFVRKGAGG"
BASE COUNT         1022 a         1529 c         1643 g         1073 t
ORIGIN      
        1 ggttgacggc gccgatgatc cgtgtataaa acctgttgaa aaaacgtatc ttctgtgtga
       61 tttcgtgcgg cttctctttc acttccatca catcctttcg ttttggattt tcactctgta
      121 atgatatttc ttatcattga tgctgtcaag caaattatcg cccgtttgga agaaattttg
      181 gtggaaatac aaccctttcg gtgaggagct cgagatgtgt atgcacatat atcatcgatg
      241 cttcgttaaa tcctttagga attgaagttt ttcgggagag atattccctg agaggaatga
      301 cgagtagacc gtcttgttgt gcagcagcgg ctcgctccga agttcgggga gaatggaggc
      361 ggcttcctcg aacgagggcg ttcccggcac aggggagaat tccgcaagct ttgccctcgc
      421 gccgcatgag tcgacgaagc gtatcgacgc ttctacggag acggtcgtct gcccgggaag
      481 gccggcgaga atgtacgttt ccatctgttc gggtgcgaag cccgcctttt gaaggttgcg
      541 aagggcgtgt tggaactctt ctccggacgt cttgccggag ccctttcgtt gaaaagcggg
      601 gtcgctgctc tcaagactca ggcgcagcgt atgaaaccct gtttgcttga ggacagcggc
      661 gcagcgttcg tcgatctccc ggatgtggag cccgttcggc gtatggtagc gaacgccggg
      721 gaaacgacgg acgagttcct cgcagagagg gtagaagcgc cgttccttgt cgatcagcag
      781 cgcgtcgtcg tagaatgccg cgtcatgcac tccgtcgagg gagaactgaa aggcggcgtc
      841 ggcaagcgtt tcggaaaggt ccctctcgat gaactgcggg aagagcagct tggaagcgca
      901 gtactcgcag gagagcggac agccgaacga cgtcagaagc agcgcgtacc ccggctcgga
      961 gtacagctcc atcgccgggc ggacgaaggg gatgtccggc aggatgtcct gtacgatgtc
     1021 cgctcctgaa cgtccggcgt gttcggggca gagccgggca tacactcccc ccaaaaggac
     1081 aggaacgccg gggaagacct ccttcgccag ttcgacggct cgaaagacgc cctcatacca
     1141 gtaggtcata ccggacgtga ccatgatgca gtccggacgc gggagcgcat gcagacgcag
     1201 gagaatctcc gcgtcgtcca agccgaacct gtggtagcgc cgggggatgt tccggaaggg
     1261 agccggtttg gagatttccc tccggaggag cttcctgcgt ccgaaggaga gctgctcgcc
     1321 ggagccttcc gcgatgcagt cgagaagatg gactgtattc ccccgtctcc tgagagcgtc
     1381 gaggagaaag aggagcccga gcggtttggc ccacaggtcg aaaaacgtga agtcgaagac
     1441 ggggggattg atcccgagaa tggtcgctcc ccgaagcccc ctgagaaacg gcgttgcatc
     1501 cgccatgaat gatcagctct tttcagaaga tatggaggaa gtcttggcgg cttctccttc
     1561 tttaataata ccatcgcgcc gacggacgca acgaaggcgc cgcgtcggcg aacctctccc
     1621 cgtgcagaga gtccggccgt ttttgacagt atttcttggc aaggtgtata attgcggcgt
     1681 tcgcatcggg ggagtccgca acggactgag aggggggtgt tcgtcccccg actcctggaa
     1741 cctgatccgg gtcatgccgg cgaagggaag gtgccgcttt cagggacatt ccaccgatga
     1801 aaagttgaaa ggaggagtgt tccatgtttc atgtccgcaa tactattctc gtcgaaggcg
     1861 ctctcgcagt cgccctctcc gttgtgttct cggcgcttcg tctctggacg atgccccagg
     1921 gaggctccat caccctcgaa atgcttccga tatttctttt cgccctcagg cggggcggaa
     1981 aagcaggatg tgcggcggga gccgtctcgg gcgttatgca gctcatcacc ggcgggtaca
     2041 tcgtgcaccc cgcacaggct cttctggact atccgctcgc gttcggcgtc ctcggcgtaa
     2101 cgggtttctt caaaaaccgt cctctctggc tggggataac gctcggcggc gtgcttcgct
     2161 tcttctgtca cgttctctcc ggagtcgtct tcttcggctc gttcgccccg gaagggacga
     2221 acgtctggat gtactccgca gtctacaacg gctcgttcat ggccccgacg ctcgtcgtct
     2281 gcggcgtcct cgcctatctt atctggccca ggctcaggag ggtgggggcg gagggatagt
     2341 tccctcgccg aagagccgct cccgcatctt tctgcttccg gcgggcagag tcgccgccgc
     2401 cagttccgac gggcttgccc acctgccgtt catgttttca aggtcgccgg gagcatgcag
     2461 ccgtacaatc cggactgcga ggcgaagacg ccacttcgta aaggagcagc gaacttcgcc
     2521 gccgcagtcc gcgttgcttc tgcgcgccag ttcggtcaac ggcgattcct gctccgcagg
     2581 aggcgcgctc caggggaact cctcgaagtt cgcccacagt cccgcagggg ggcgtcttcg
     2641 cagaaaaacg cgatctccgg acacgaagac ggcaaggatt ccttcgaggc tctccgactt
     2701 cgtctgccgg gagagtaccg ggcgttcgag ggcgcttccc cgaagccgcg cgagacagag
     2761 gaattcgagc ggacactctc cgcacagggt tgacgtaggc gcgcagagcg tcgcgccgag
     2821 ctccatgatt gcggagttga agtcccccgg acgatccgcc ggaagcattt gcacaaaggc
     2881 cctcaggacg ctcttttccc ctgccctgcg gtcgaccggc tcttcaaggt cgagaagccg
     2941 ggagaagacg cgcttcccgt tggcgtcgag ggctggaacg ggaagaccga aggcgatgct
     3001 cgccacggcc gcggcggtgt aggggccgat gcccggaagg gcccgcagct cctcgaagga
     3061 cgcgggaagc gtagcaagtc cgcgcgcttg cagcagccgc gccgcacgaa gcagatttct
     3121 cgctctggag tagtagccca gcccttccca tgctttgaga acgtcatcgt ccttcgcttc
     3181 ggcgagcgag cgcagcgtgg gaaaggtgcg catccagcgg cgatggtact ccacgcctcg
     3241 tgcggcttgc gtctgctgga gcatcacctc cgagatccag acggcgtacg gatcgttcgt
     3301 ctcccgccag ggaaagggcc gccggcaggc gtcgtaccac cgcagcaacg gcgccgcccc
     3361 ttcttccaga gtgatgttcg gcgaactgtg taccatgcag cgcgctcctt ccttcttcat
     3421 ctcttcgttt ttcttccata gagcatatgt ataacatgga gcgtcatagg gtacaatacg
     3481 aggggagtat tccggatatt tcaccggctc cgaaaagccc tggccataga ggctttcccc
     3541 ccgttcgttc gagggggcgc gctctatccc gcagaaggag gaacggcaat ggcgaagaca
     3601 gaaaacatac ggtacgattt ttctcttttc ggcgagatgg actcatatct cttccgggag
     3661 ggcaatcact tccgcctgta cgaaaaactg ggagcgcacg tcgtcgacga cgggaaactt
     3721 ccggggacgt atttcgcgct ctgggcgccc aacgcgcggg aggtcagcgt gatcggcgcc
     3781 ttcaacgact gggacaggac gcgccactat ctctccccga gaaaggataa ctccgggata
     3841 tgggagggtt ttgttcccgg agtgggcaag ggcgcgctgt acaagtattc cctccgctcc
     3901 cgcaacggga gcgtcttcga gaagggagat cctttcgccg ccttctggga gatcgcgccg
     3961 aagacggcct ccatcgtctg gccctgcgac tatgcctgga acgacgggga gtggatgaag
     4021 aaccgccgcg cgcagaacgc tctcaatgcc ccgatgtcgg tctacgaggt gcatgtcggg
     4081 tcgtggcgca gaaaaccggc cgagggcttc cgttcgctct cctaccgcga gctggggcat
     4141 gagctcggcg agtacgtccg cgcggccggc ttcacgcacg tcgagctgct ccccgtcatg
     4201 gaacaccctt tctacggctc ctgggggtat cagacgctgg gctacttcgc ccctaccagc
     4261 cgttacggaa cgccggagga cttcatgtac atggtggatg tgctgcatca gatggggatc
     4321 ggcgtgatcc tcgactgggt gccgtcgcac tttccggccg acgcgtacgg tcttgcacac
     4381 tttgacggca cctgcctcta cgagcacgag gatccccgga aggggtacca gccggactgg
     4441 ggcagccaga ttttcaattt cggccgcaac gaggtgcggg agttccttat cagcagcgcc
     4501 ttctgctggc tcgacctctt tcacgccgat ggaatacggg tggacgccgt cgcttccatg
     4561 ctctacctcg attactcccg aaaggaaggg gagtggatcc cgaaccagtt cggcggcagg
     4621 gagaatctgg aagccgtcgg ctttctcaaa aagatgaacg aggcaatcta ccaggagttc
     4681 cccgatgttc agacaatcgc cgaggagtcg acggcctggc cgatggtgac gcggccgacc
     4741 ttcatcggag gactcggctt cggcatgaag tggaacatgg ggtggatgca cgacattctt
     4801 gagtacatgc agaaggaccc tgtctaccgt aagtacaacc acaacaagat caccttcagc
     4861 attatgtacg cctttacgga gaactttctc ctccccttct ctcacgacga ggtggttcac
     4921 ggaaaagggt ccatgctcgg caagatgccg ggcgacgaat ggcagcagta cgccaatcta
     4981 agactgctgc tggggtatat gtacgcgcat ccggggaaga agctcctctt catggggagc
     5041 gaattcggcc aggggaggga gtggaaccac gacgacagcc tcttctggga tctgctgcac
     5101 tctccccgcc acaaggggat gctggactgg gtgacggatt tgaaccgggt ctacaggagc
     5161 gaagcagccc tccattcgcg tgacttctcg ccggaggggt tccagtggtg cgacttctcc
     5221 gactgggagc agagcgtcgt ctccttcgtc aggaagggcg ccggggg
//
LOCUS       DAOSWW010000055         5168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10763_length_5168_cov_3.543321, whole genome shotgun sequence.
ACCESSION   DAOSWW010000055 DAOSWW010000000
VERSION     DAOSWW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10763_length_5168_cov_3.543321"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="PK442_01985"
     CDS             <1..1636
                     /locus_tag="PK442_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOO86447.1"
                     /translation="EAVAVMLFYESGELVQDLAVAKSRRSILSLMDIRPDSAVLLEEG
                     GERRVRAEDVRVGETVLVKPGERIPLDGSVKTGTSSLDTSSLTGESVPRDVAPGDDVL
                     AGSVNLGGMLTVLVTRLFGESTLSKGLRLIEEARERKAPTERFITAFAKRYTPAVVAA
                     AALLAVVPPLMGLGAFREWGYRALIFLVVSCPCALVISVPLALFGGIGAASRRGVLLK
                     GGNVLETLAKVRTVLFDKTGTLTRGVFRVTGVFQAGNTDRERLLSIAGEAERGSNHPV
                     ARAVVEAAGALPPLGGAIEELPGRGVRLVGGGKVILAGNARLLTDSGIASAPEAEGRP
                     GAVVHVAEDGRYAGYIVVEDTVRSQSKRAVERLRSLGVSTVGMLSGDRRENASRIAED
                     LGLDLWESDLLPSDKLEHFQRISERTGGTTLFVGDGMNDAPLLAAADVGMAMGGLGAD
                     AAIEAADGVILNDDPLGAADVIEIARKTRSIVWQNIAFALGVKGAVLTLGAFGMATMW
                     EAVFADVGVALLATLNAARVLRFGERMQREAEEIKV"
     gene            complement(1748..2920)
                     /locus_tag="PK442_01990"
     CDS             complement(1748..2920)
                     /locus_tag="PK442_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO86448.1"
                     /translation="MKPNSPAAGSVSLFLNIFGRCDFLFALLLGNAGQVLAVNDRAAA
                     LLGTEENCCPLPLAELLAEGQEDCHARIEEAASGESLPLYLKTAAGSRIGIDCSVAAL
                     GEEMLVFGMYSDQENDRIITEMSVLNLEMANMTRELHKKNLQLAVSQQALAKADSMKK
                     TFLAIIAHDLRGHVGGVSQLLSMLQQDYDDMDDAEKKECVTLASDEARRTFLLLEDLL
                     SWSRSQLDQIGFTPVDCNLRALIEENIALLQSRAKEKGVALSHETPPSLSATLDRSMM
                     GTVLRNLLNNALKFTAPGGRVLVSASQKGESVEIIVRDTGTGMSQEQLENLFRIDAKP
                     VSTQGTSGEKGSGLGLILCKEFVEKNGGMIRAESVAGEGSSFFISLPSARGASDGA"
     gene            complement(2907..4025)
                     /locus_tag="PK442_01995"
     CDS             complement(2907..4025)
                     /locus_tag="PK442_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOO86449.1"
                     /translation="MASALETSALVPVSQKAAQDYAAHADNLLNYVDACMKKAPLDAL
                     AAKQVEMMLQNHRNHLQFMMSFFRLNNPELLKEIVIWVYRAYQAHGFSPDYFPAVLRR
                     WMEAVSQFLPPESAPEILGVYGWMLENHQRFLAAADEYRHMEQEAAGAPHPLQALRNE
                     FLQGLIAGSLSRCNACATGYITGKDELREFYLGVVQPAMIEIGNLWERGELSVAREHL
                     ATAIVTRIMAAQHMRFFGLEKPKGKALISAAANEFHELGARMVADLFDLDGWDVTYIG
                     SNVPVEAAEDMIRNEKPSVVGLSVTMPFNLGKTADLVDRIHSSPELAGVKIIVGGRAF
                     QFSPTLWQTIGADAYANNAIEALNAANSFMETRPREAE"
     gene            complement(4245..>5168)
                     /locus_tag="PK442_02000"
     CDS             complement(4245..>5168)
                     /locus_tag="PK442_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86450.1"
                     /translation="LDRLLLMLSSPDRSGGASMAPAISAAAPSGAQAKLTKKIIAGVP
                     TQLTYTVVVQNRSGVPVVNPAVKDNGKPLNNDDAVKTGGNQDNILEIGETWTWTYTVT
                     VTGKTGERILNTVTVEGPDSTIRDTDQGNNTDETVVEVDPAPGNYDLSVSKTVAVTGQ
                     ADTGLPPGINPTDVVDSPPQTITPDDCGKWLRSASGGYKGTRKQYDIASLPSGTTFDL
                     DFQAFDVPDKIEVYYPAGTKVFDSGWRGDMNHIQKKPSVANLYPGGVVGTGKLLQNGL
                     FTKSGANEFLVIVYGPEQGTAWEFSVKANCP"
BASE COUNT          927 a         1468 c         1641 g         1132 t
ORIGIN      
        1 cgaggccgtg gcggtgatgc tcttttacga gtcgggggaa cttgtgcagg atttggcggt
       61 ggcgaagtcc cgacgttcca ttctctcgct gatggatatc cgtcccgatt cggcagtgct
      121 tctcgaagaa gggggagagc ggcgcgtgcg tgcggaggac gtccgggtgg gggagaccgt
      181 ccttgtgaag ccaggcgaac gaattcctct cgacggttca gtgaaaacgg ggacatcatc
      241 gctggatacg tcgtcgttga cgggcgaatc cgttccgcgg gatgttgcgc ccggcgacga
      301 cgtgcttgcc ggaagcgtca acttgggcgg gatgctcacg gtccttgtga cccgtctctt
      361 cggcgagtcc acgctctcga aagggttgcg ccttattgaa gaggcaaggg aacgaaaggc
      421 gcccacagag cgcttcatta ccgcctttgc aaaaagatat accccggcgg tggttgccgc
      481 cgccgcgctg ctcgccgtcg tgccgccgct gatggggttg ggcgcgttcc gggaatgggg
      541 gtaccgcgcg ctcatcttcc tcgtcgtctc ctgtccgtgc gcccttgtga tctccgtgcc
      601 gctcgcgctc ttcggcggta tcggagccgc ttcaaggcga ggcgtgcttt tgaaaggtgg
      661 aaatgtgttg gagacgctgg cgaaagtccg caccgtgctt ttcgacaaga cgggtacgtt
      721 gactaggggc gtgttcaggg tgaccggagt cttccaggcc gggaatacgg accgggagag
      781 attgctttcg atcgccgggg aggcggagag agggtcgaat cacccggttg cgcgggccgt
      841 tgtagaggct gccggagcgc ttcctcctct cggcggggcg atcgaggagc tccccggcag
      901 gggcgttcgg cttgtcggcg ggggaaaggt aattctcgcg ggaaacgctc gcctgttgac
      961 cgactccggc atcgcctcgg ctccggaggc tgaagggcgc ccgggggccg tggtgcatgt
     1021 cgccgaggac ggccgatacg cagggtatat cgtcgtcgag gatacggtgc gctcgcagtc
     1081 gaaaagagcg gtggagcgcc ttcgttctct cggggtgtca actgtcggga tgctctcggg
     1141 cgaccgtcgg gagaacgcct cccgcatagc ggaagatctc ggactggatc tctgggagag
     1201 cgatcttctt ccgtccgata agctggaaca cttccagcgg atcagcgagc gaacgggagg
     1261 cacgacactt tttgtggggg acggaatgaa cgacgctccg ctgctcgccg ccgcggatgt
     1321 gggcatggcc atgggcggcc tgggagcgga cgccgcgatc gaggccgccg acggcgtcat
     1381 cctgaacgac gatccgctcg gcgcggccga cgtcatcgaa atagccagga agacgcggag
     1441 catcgtctgg cagaatattg cctttgcact cggggtgaag ggagctgtac tcacgctggg
     1501 agcgttcgga atggcgacca tgtgggaggc cgtatttgcc gacgtgggtg tcgcgcttct
     1561 tgcaacgctc aacgcagcgc gggttctgcg ctttggagag cgcatgcagc gcgaagcaga
     1621 ggaaattaaa gtatgaagag gtagatgaat actgaccaaa agagtggaga aaagaggccg
     1681 cagcgcgtcc cgaactgggg gttgctgcgg ccgttttgct gttgcgtttt gtgtaggtgt
     1741 tttattatta cgctccgtcc gatgctccgc gtgcggatgg cagcgaaatg aaaaaggagc
     1801 tcccctctcc ggcgacgctc tccgcacgaa tcatgcctcc gtttttttcg acgaactcct
     1861 tgcagagaat aagaccgagt ccgctccctt tctcccccga cgtcccttgt gtgctgacgg
     1921 gtttggcgtc gatgcggaag aggttctcta gctgctcttg cgacatgccg gttccggtat
     1981 cgcgtacgat gatctcgacg ctctctccct tctgggatgc ggaaacgagg acgcgccctc
     2041 cgggggcggt gaacttcagc gcgttgttca gcagattgcg gagcaccgtt cccatcatgc
     2101 ttctatccag cgtggcggaa agggaaggcg gcgtctcgtg agagagggcg actcccttct
     2161 ccttcgcgcg ggattgcagc agggcgatgt tctcttcgat cagcgcgcgc aggttgcagt
     2221 cgacgggggt gaatccaatc tggtcaagct gcgatctcga ccaggagagg aggtcttcga
     2281 gaagaaggaa cgttctgcgg gcttcgtccg aggcaagcgt aacacactcc ttcttttccg
     2341 cgtcgtccat gtcgtcatag tcctgctgaa gcatcgagag cagttgactt acgcctccca
     2401 catgacctct gaggtcgtgg gcgatgatgg caagaaacgt ttttttcatc gaatcggcct
     2461 ttgccagcgc ctgctgggag acagcgagct gaaggttttt cttgtggagt tccctggtca
     2521 tgttggccat ctccaggttc aagaccgaca tctctgtaat gatccggtcg ttctcctgat
     2581 ccgagtacat accaaagacg agcatctcct caccaagcgc ggcgacgctg caatcgattc
     2641 cgatgcgcga gcctgccgcc gtcttcaggt agagcggcag actttcgccg gaagccgctt
     2701 cttcgatgcg cgcatggcag tcctcctgtc cttccgcgag gagttccgcc aaaggcaggg
     2761 ggcagcagtt ctcttctgtt cccagaaggg cggcggctct gtcgttgacc gcgagaacct
     2821 gtcccgcgtt cccgagaagc agcgcgaaga gaaagtcgca ccgtccgaag atgttcagaa
     2881 agagagagac gctgcccgcg gcagggctat tcggcttcac gtggccgtgt ctccatgaaa
     2941 ctgtttgccg cgttcagggc ttcgatggcg ttgttcgcgt atgcgtctgc gcctattgtt
     3001 tgccataagg tcggcgagaa ctggaacgcc ctgccgccga cgatgatctt cacgcccgca
     3061 agctccggcg aggagtgaat acggtcgacc aggtcggctg tcttcccgag gttgaagggc
     3121 atcgtcacgg agaggcccac gacggacggc ttctcgttgc ggatcatatc ttccgccgcc
     3181 tccacgggga cgttgctgcc gatgtacgtc acatcccatc cgtcgaggtc gaacaggtcg
     3241 gcgaccatgc gcgcgcccag ctcgtggaac tcgttggccg cggctgaaat cagcgccttc
     3301 cccttgggtt tctccagacc gaagaagcgc atgtgctgcg ccgccatgat cctggtgacg
     3361 atggctgttg cgagatgttc ccgcgctacc gaaagctcgc cccgttccca cagattgccg
     3421 atctcgatca tcgccggctg cacgacgccg aggtaaaact cccgtagctc gtcttttcca
     3481 gtgatatacc cggtagcgca ggcattgcag cgggagagac tgcccgcaat cagcccctgg
     3541 agaaactcgt tccggagcgc ctgcagcggg tggggcgctc cggcggcctc ttgttccatg
     3601 tgtctgtact catcggcagc cgcgagaaag cgctggtggt tttccagcat ccagccgtag
     3661 acgccgagta tctccggtgc gctctccggc gggaggaatt gcgaaaccgc ctccatccag
     3721 cgtctgagga cggcggggaa gtagtcggga gaaaatccat gggcctgata cgcccgatag
     3781 acccagataa caatctcttt gagcagttcg gggttgttca ggcgaaagaa cgacatcata
     3841 aattggaggt gattgcgatg gttctgcagc atcatttcaa cctgtttcgc cgcgagagcg
     3901 tcaagcgggg cttttttcat gcacgcgtcg acataattca gcagattgtc cgcatgcgcc
     3961 gcatagtcct gcgccgcctt ttgcgaaacc ggaacaagag cgcttgtttc aagtgctgat
     4021 gccatcgaac tcaactcctt ctcgtggacg aaaatggatg gaacagctgt tatggtaggc
     4081 tttttagaag tgggtgtcaa tccggcccgc gtgttccgta ttcctttccc ggcctccggc
     4141 gcggcatgct tcaggaaaaa gcccccggcg tccggagagt tttggacgcc gagagctttt
     4201 ttcgaatttc atagcatctg tcctggagcg tttttctccc aagactaggg acagttggcc
     4261 ttgacgctga actcccaagc ggttccctgt tccgggccgt agacgatcac caggaattca
     4321 ttcgcgccgc tcttcgtgaa aagaccgttc tgcagaagct tccccgttcc gaccacgccg
     4381 cccggataga gattggccac gctcggtttc ttttggatat ggttcatatc ccctcgccat
     4441 ccgctgtcga aaaccttggt tcctgcagga tagtaaacct ctatcttgtc cggcacgtcg
     4501 aaggcctgga agtccaggtc gaaggtcgtt cccgaaggca gagaagctat atcgtactgc
     4561 ttccgcgttc ctttgtagcc gccgctggcg cttcgcagcc actttccgca gtcgtccgga
     4621 gtgatggtct gcggcgggga gtcgacgacg tccgtcggat taatgcccgg aggaagtccg
     4681 gtatcggcct gtccggtgac ggcgacggtc ttggagaccg aaaggtcata gttccccggc
     4741 gccggatcga cctccacgac ggtttcatcc gtgttgtttc cctggtcggt gtcgcggatc
     4801 gtagaatccg gtccctccac cgtgacggta ttgaggatgc gctctccggt tttccccgtc
     4861 acggtgactg tgtacgtcca tgtccacgtc tcgccgatct caaggatatt gtcctggttg
     4921 ccgcccgttt tcactgcatc gtcgttgttc aaaggtttac cgttgtcttt taccgcgggg
     4981 ttgacgacag ggactccgga ccggttctgt acaacgacgg tgtaggtgag ctgcgtgggg
     5041 acgcccgcga ttattttttt cgtcagtttt gcctgcgcac ccgagggagc ggcagccgat
     5101 atcgcgggcg ccatagaggc gcctcccgat cggtcagggg aggagagcat caagaggagc
     5161 ctgtccag
//
LOCUS       DAOSWW010000056         5160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10792_length_5160_cov_3.737708, whole genome shotgun sequence.
ACCESSION   DAOSWW010000056 DAOSWW010000000
VERSION     DAOSWW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10792_length_5160_cov_3.737708"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK442_02005"
     CDS             complement(<1..414)
                     /locus_tag="PK442_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86451.1"
                     /translation="MHWKDSSTWKLFKYSLLSYLLLAGLFTMLYSLFAYSREKDEMER
                     TLYTVRRTHVPNIVDALWITDTQRVQRELDAIARFEFVDRAEVRDDEGRILFAGSEPL
                     LEAQLRTETLRYAHDDRSVVIGELSVFLGVRRMALK"
     gene            complement(429..1172)
                     /locus_tag="PK442_02010"
     CDS             complement(429..1172)
                     /locus_tag="PK442_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO86452.1"
                     /translation="MFRSLAAVCLWIFLLLPAPETARAGERVFRISTSYKSLLSTPEQ
                     TGMLDMLLKEAFRRSGLRAEIVFTPTERSMYDVDAGLLDGEINRIEGMEKRFPNLVRV
                     PEPNMTMHFVAFSNRPYPVDGWESLKNLHVGVVSGWRILEEHTVDFPKRILVPSETEL
                     FTMLHKGRIDVALYDKLTGYETISRLGFPEIRHLEPPLINRDMFLYMHKSHSEVVGTI
                     AEALRGMKLDGAYDRIVSEATARLGADAR"
     gene            complement(1440..2192)
                     /locus_tag="PK442_02015"
     CDS             complement(1440..2192)
                     /locus_tag="PK442_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO86453.1"
                     /translation="MILSVNGISFAYRGVPVLDGVSFSLAEGEMVALLGPNGTGKTTL
                     LRTINGILRPKGGTVLVESRDASTCSPRERAKFFGYVPQRGEAVRLTVFDAVLLGRRP
                     HLGWTVERRDLEKVESVLETLSLEKFTLRYLDELSGGEFQKVLLARALVQEPRVLLLD
                     EPTSSLDLKNQLDMLATLQRVVRGHNVAALLSLHDLNTAFRFADRLLFLKDGVIRGSC
                     APSDAPEALIREVYDVPVDIVSHKGIPMIVPS"
     gene            complement(2189..3256)
                     /locus_tag="PK442_02020"
     CDS             complement(2189..3256)
                     /locus_tag="PK442_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO86454.1"
                     /translation="MHLEGHHISPAYEHHVQRKGLFLFGLAAACVLMVVFSISRGAVS
                     IPPLDVVRTLLGGGEAKWRIIVLNIRLPQTLAALLAGGGLSLAGAAMQSVLRNPLGSP
                     FTLGISNAAAFGAAFSIIVLGTGSMQSSAVNAVTIVNPWLTTGSAFIAALLCTGVVLV
                     ITWVTRGTPDVMILAGVALSSLFTAGTMFLQYFADDSQLAATVFWTFGDVSRASWRDI
                     PLMGLLLLGAFCLFFLRRFDLNAIDCGDETALSLGVSAPRIRIETMVVSSLLSAVMVA
                     LLGVIGFVGLVCPHMLRRFLGSDHRFLIPGSVLMGGLLLLAADTGARLAFAPRTLPVS
                     VFTAFLGAPVFLALIARRKRT"
     gene            complement(3261..4379)
                     /locus_tag="PK442_02025"
     CDS             complement(3261..4379)
                     /locus_tag="PK442_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HOO86455.1"
                     /translation="MFRKAPLTSLCLLFCLFALPASAAEMTVTDVLGRSVTVPASPER
                     IIASGSGALRLIVYLQAQDRVVAVDSAERRVPNLAVLVSARPYSIANPQFQDMPVFGE
                     FRGMDNPELIAGLSPQPQVIFKVSPLSGPHPDQLTAKTGIPVVGLEYGNLTDKKSDFY
                     ATLRTMGQILGKSERAEEVVAFFERHLAELSRRTADIPDDKRPACYIGGVASRGAHGF
                     TSTEPGYPPFLYTNAKNVAALPGEKTSAVAQISKEKLLEWDPEILFVDLSTTTAGEQA
                     SSLYQLTHDPALSALSAVREGNIWGVLPYNSYTLNYGSVLANGYFVGKTLYPDRFKDI
                     DPAATADEIFTFLVGQPVFSMMNEGFSGSAFSKIALGE"
     gene            4548..5111
                     /locus_tag="PK442_02030"
     CDS             4548..5111
                     /locus_tag="PK442_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOO86456.1"
                     /translation="MESLEKIVAENLKRLRGERKLSLDRVSALTGVSKSMLGQVERGE
                     SSPTLQTVWKIANGLRIPLSELTDAPKQETESFSKEQVAAILGDNGKFRVYPIFPFDG
                     KNRFEFLSIEMDRGAFSSSEPHIAGTVEYVHVFEGEVVIMTGSEERRLECGDSMKYRA
                     DRPHSYHNPGRERAVLAMVILYPEENV"
BASE COUNT         1042 a         1488 c         1704 g          926 t
ORIGIN      
        1 cttcagcgcc attctgcgga cgccgagaaa gacggagagt tcgccgatga cgacggagcg
       61 atcgtcgtgt gcataccgaa gcgtctcggt ccggagctgc gcttcaagaa ggggctccga
      121 tccggcgaag aggatgcgcc cctcgtcgtc ccgtacctcg gccctgtcga cgaactcgaa
      181 gcgcgcgatc gcgtcgagtt cgcgctgcac tcgctgcgta tccgtgatcc agagtgcgtc
      241 gacgatgttg ggaacgtgcg tccggcgtac tgtatagagc gtccgctcca tctcgtcctt
      301 ttcgcgcgag tatgcaaaga gagagtagag catcgtgaag agccccgcga gcagcaggta
      361 tgagagtaag gaatatttaa atagcttcca cgtgctcgaa tccttccagt gcaatggaac
      421 tcgtcctttc aacgggcgtc cgcgcccagg cgggcggtcg cctccgagac gatgcgatcg
      481 tacgcgccgt cgagcttcat cccgcgcagc gcttcggcga tcgttcccac tacttcggaa
      541 tggctcttgt gcatatagag aaacatatcc ctgttgatca ggggaggctc cagatggcgg
      601 atctccggaa aacccagacg ggagatcgtt tcgtaccctg tcagtttgtc gtagagagcg
      661 acgtcgatcc tccccttgtg gagcatggtg aagagttccg tctcgctcgg aacgaggatt
      721 cttttcggga agtccacggt atgttcttcg agaatgcgcc atccgctgac gacgccgacg
      781 tgcagatttt taaggctctc ccatccgtcc acgggatacg gccgattgga gaaggcgacg
      841 aagtgcatgg tcatgttggg ttcgggtacc cgcaccagat tggggaatcg tttctccatc
      901 ccctcgatcc ggttgatctc tccgtccagc agtcccgcgt cgacgtcgta catggagcgc
      961 tccgtgggcg tgaagacgat ctccgcccgc aggccggacc tccggaaggc ctccttgagc
     1021 agcatatcca gcattccggt ctgttccggg gtggagagca ggcttttata cgatgtgctg
     1081 atgcgaaaga cacgctcacc cgccctggct gtttccggag ccgggagtaa aagaaaaatc
     1141 cagaggcaga ccgctgcaag agacctgaac atgacgtcac ccctttgtcg ttgatactcc
     1201 gccggccggc cccgtacggg gaaaacaagg acggaagcgg agaaggcagg tctattttta
     1261 ccatgaactc cccgtaaata caatttcaat cgagtgcttc tccgaaaaaa gaataatttg
     1321 taggcggacg ggcttgaaaa caatcgacat gtttccgtcg acttttcaag cccgtccgtt
     1381 tggttgctcc gtatatgggg tgttgaagac aggctttcgc ctttatttcc gtgttgtgct
     1441 catgacggta cgatcattgg gatgcccttg tgggagacta tgtccaccgg gacgtcgtag
     1501 acctcgcgga tcagcgcctc gggagcgtcc gacggcgcgc aggagccccg gatgacgccg
     1561 tccttcaaaa agagcaggcg gtcggcgaag cggaaggcgg tgttcaggtc gtgcaggctc
     1621 agcagcgcgg cgacgttgtg gccacggacg acccgttgca gcgtggcgag catgtcgagc
     1681 tggttcttca ggtcaaggct gctcgtcggt tcgtcgagca agagtacgcg cggctcctgg
     1741 acgagcgccc gggcgaggag caccttctgg aactcgccgc cgctgagttc gtcgaggtag
     1801 cggagggtga acttttcgag cgagagcgtt tcgaggaccg attcgacctt ttcgaggtct
     1861 cgccgctcca cggtccaccc caggtgcggt cgacggccga gaagcaccgc gtcgaagacc
     1921 gtcaggcgca cagcctcacc ccgctgcggg acgtagccga agaactttgc ccgttcgcgc
     1981 ggggagcatg tagaggcgtc cctcgattcg acgaggaccg ttcccccctt ggggcggagg
     2041 atgccgttga tcgtccgcag cagcgtcgtc ttccccgttc cgttcgggcc gaggagcgca
     2101 accatctctc cctcggcgag cgagaaactc accccgtcca gtaccggcac gccacggtag
     2161 gcgaaggaga ttccgttcac cgagaggatc atgtccgctt cctccgtgcg atgagcgcga
     2221 ggaagaccgg agcgcccaga aacgccgtga agaccgacac cgggagcgtg cgcggcgcga
     2281 aggcgagacg cgcccccgtg tccgccgcca gcaggagcag cccccccatc agcaccgagc
     2341 cggggataag aaatctgtgg tccgagccca gaaagcggcg gagcatgtgc ggacagacca
     2401 gtccgacgaa gccgatcacg ccgagcaggg ccaccatcac cgccgagagg agcgaggaga
     2461 ctaccatcgt ttcgatgcgg atgcgcggcg cggagacccc cagcgagagg gctgtctcgt
     2521 cgccgcagtc gatggcgttg aggtcgaagc ggcgcaggaa gaagaggcag aaggctccca
     2581 gcagcagcag ccccatcagc ggaatgtcgc gccacgacgc ccgcgagacg tccccaaaag
     2641 tccagaagac cgtcgcggcg agctgactgt cgtccgcgaa gtattgcagg aacatcgttc
     2701 ccgccgtgaa gagggagctg agggcgaccc cggcgaggat catcacgtcc ggcgttcccc
     2761 gcgtgaccca ggtgatgacg agcaccactc ctgtgcagag aagcgcagcg atgaatgccg
     2821 agccggtggt gagccacgga ttgacgatgg tgacggcgtt gacggcgctg ctctgcatgc
     2881 tgcctgttcc cagcacgatg atggagaatg cagcgccgaa ggccgccgcg ttcgagatgc
     2941 ccagcgtgaa gggcgagccg agcgggttgc gcagcaccga ctgcatcgcc gctccggcga
     3001 gggagagccc ccctcccgcc agcagcgcgg cgagcgtctg cggaaggcgg atgttgagca
     3061 cgatgatgcg ccacttcgcc tctccgccgc cgagaagcgt gcggaccacg tcgagagggg
     3121 ggatggagac ggcgcccctt gatatggaga agacaaccat cagcacgcag gcggcggcca
     3181 gcccgaagag gaagagtccc ttgcgctgca cgtggtgttc ataggcggga gagatatggt
     3241 gtccttccag atgcacgtcg ctactccccg agtgcgattt tcgagaatgc gctccccgag
     3301 aacccctcgt tcatcatcga aaagacaggc tgccccacga gaaaggtgaa gatctcgtcg
     3361 gccgtcgccg ccgggtcgat gtccttgaag cggtcggggt agagcgtctt cccgacgaaa
     3421 tagccgttgg cgagcaccga gccgtagttc agcgtgtacg agttgtaggg cagcacgccc
     3481 cagatgtttc cctcgcgcac ggcggaaagc gccgagagcg cggggtcgtg cgtcaactgg
     3541 tagagcgagc ttgcctgttc gcctgcggtc gtggtgctca agtcgacgaa gagaatctcc
     3601 ggatcccatt cgagcagctt ctccttggaa atctgagcaa cggcggaggt cttctcgccg
     3661 gggagcgcgg cgacgttctt cgcgttcgta tagaggaagg gcgggtatcc cggttccgtc
     3721 gaggtgaagc cgtgcgcgcc gcgcgaggcc acgcccccga tgtagcacgc gggacgtttg
     3781 tcgtcgggga tgtccgcagt ccggcgggag agttcggcga ggtggcgctc gaagaaggcc
     3841 acgacctcct ccgcccgttc gctcttgccg aggatctgtc ccatggtgcg gagcgtggcg
     3901 tagaagtcgc tcttcttgtc ggtgagattg ccgtactcaa gccccaccac cggaatgccg
     3961 gttttggccg tgagctggtc ggggtgcggg ccggagagcg gggagacctt gaagatcacc
     4021 tgcggctgcg gcgagagccc ggcgatcagc tccggattgt ccattccgcg gaactcgccg
     4081 aagaccggca tgtcctggaa ctgcgggttc gcgatggagt acgggcgggc cgagacgagg
     4141 acggcgaggt tgggaacgcg ccgttcggcg gagtcgacgg cgaccaccct gtcctgcgcc
     4201 tgaaggtaga cgatcaagcg gagcgctccg gaaccggagg cgatgattcg ctcgggcgag
     4261 gcgggtactg tcacggagcg gccgaggacg tcggtcacgg tcatctccgc ggcggacgcg
     4321 ggtagggcaa acaggcagaa aagaagacag agagaggtca agggtgcttt gcgaaacacg
     4381 gaaatccact ccttataaaa aacatgttca gtgcgcaggt aatagtagca cgaaaatgtt
     4441 tatttgtgtt atcgagttgc acccttcgtg ggaaatctct tgacagaaac gttctttttg
     4501 tgcaatataa cgcacataaa gaacgctgtg tcagggaggc aagagctgtg gaaagtctcg
     4561 aaaagatcgt cgcggaaaat ttgaagcgtc tgcgggggga gcggaagctc agccttgaca
     4621 gggtgtcggc ccttaccggc gtgagcaaga gcatgctggg gcaggtcgag cggggagagt
     4681 cgagcccgac gctccagacg gtctggaaga tcgccaacgg cctgcgcatc cccctcagcg
     4741 aactcaccga cgctcccaaa caggagacgg agtccttctc caaggagcag gtggcggcga
     4801 tactgggaga caacggaaag ttcagggtgt accccatttt ccccttcgac gggaagaacc
     4861 gcttcgaatt cctctccata gaaatggaca ggggggcctt ttcctcctcc gaaccgcata
     4921 tcgccgggac tgtggagtac gttcatgtct tcgagggcga ggtcgtcatc atgacgggga
     4981 gcgaggaacg gcggttggaa tgcggggact cgatgaagta cagagcggac aggccgcact
     5041 cctatcacaa cccggggcgg gagcgcgcgg tgcttgcgat ggtcatcctc tatccggagg
     5101 agaatgtctg aaaggggagt tgtttcatga gcagcagtga acgcaccgag agtgcgcggc
//
LOCUS       DAOSWW010000057         5142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10837_length_5142_cov_4.110674, whole genome shotgun sequence.
ACCESSION   DAOSWW010000057 DAOSWW010000000
VERSION     DAOSWW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10837_length_5142_cov_4.110674"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="PK442_02035"
     CDS             <1..1240
                     /locus_tag="PK442_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOO86457.1"
                     /translation="LKQASESAVPTVILCRTVMGKGVPFMENSSEFHGKAPLNDLYVQ
                     AMRDLGGDPSLLEDARNRRTSAQLPTGRHISTSSFGLDTGVPRIYTADAKKTDNRSAF
                     GSALADIGKLNYKKEGGAPLLVFDCDLAGSVKTGEFASLCPEWFIQTGIQEHATATVA
                     GAASTAGVAALWADFGVFGLSETYNQQRLNDINKANLKTVLTHVGLDVGEDGMTHQAI
                     DYVSLLRNTFGWKLAVPCDPNQTDRATRWAIKEPGCVCLAMGRSKLDVLTSAKGAPLF
                     GEGYEFRYGKAELVCEGNDGALFALGAMTGRALKAAQMLKEKGITVSVYAVSSPLLPD
                     DDALRAAAATGRIMTCEDHCVASGMGSILAARMTELRLNAAFKALGVHRYGDSGASED
                     VFAAMGLDAASLAAAFEKLL"
     gene            1253..1849
                     /locus_tag="PK442_02040"
     CDS             1253..1849
                     /locus_tag="PK442_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOO86458.1"
                     /translation="MSAQKVLLHVCCAPDATVPWADLPGEGYEVVPYWYGNNIHPAAE
                     EKRRRDTLAALSLRMGGSVLWEEYVPETWLEAASRLANEPEGGARCALCFRLQLEAAA
                     RRASAMKIPLLCTTLTISPHKNAELINSIGAECASQYGVAWLARVFRKKNGFVRSVAL
                     SKEYGLYRQSYCGCLYSMTRGGNNHGTAARGEASPVAP"
     gene            1806..2789
                     /locus_tag="PK442_02045"
     CDS             1806..2789
                     /locus_tag="PK442_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOO86459.1"
                     /translation="MEQQLVGKLPPSLLEGSVFRFCGAPRPEVIIGPGIGEDAALLQL
                     PEGKFLAVASDPIVGAREGAGKFLVHINANDIACKGGDPSFFVATLLVPSADGVPMVQ
                     ALMREIHEACLSIGAAVIGGHTELTSRYDQPVLVGTMMGPTAFVHRATDIRPGDVLIL
                     TKHAGLEGMAILANDRPDLLLPAIGPEGVEEARGWLEQISVLEEAKLLRGMARFLHDP
                     TEGGVGGGIAEIVRLSGLGVELDYGSVPFSPLTKKAVQTLDFDPLHLISSGVLLAVVP
                     QGGEADALAALREKGISGVAAGHFVEGAGNCPDDVREELWGLLERPKERRE"
     gene            2786..3892
                     /locus_tag="PK442_02050"
     CDS             2786..3892
                     /locus_tag="PK442_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOO86460.1"
                     /translation="MSFDHISRRLQRLRSVVAEEGMDVFVLLVTEGYNSESAAYISGF
                     RGSSCAVVISADRALLVTDGRYALQAKAQSPFSVVLQEQNTLQEKTAELLRGGGWTVA
                     GFEGERLSVSLFRAFEKSVPHWKDCSVLIPSLRRRKDELEIAAIAKAADIASASYEEV
                     LGLVREGMSETELNALLEYAVRKRGAEAGWKGSGFIVASGERSALPHGKATDRTFRKG
                     DIVTVDFGATVDGYMSDLTRNFSLGPLSEGAREIESVLLEAADKAAEALKPGAEGKFV
                     DSVARGIIASKGWGKHFSHGLGHGLGLEIHEAPRLSPVSKDILREGDIVTIEPGIYIE
                     GWGGMRIEDDYLVTSSGSVCISGGKGRKTAVVSV"
     gene            4021..4182
                     /locus_tag="PK442_02055"
     CDS             4021..4182
                     /locus_tag="PK442_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOO86461.1"
                     /translation="MKKYVCTVCGYVYDPAEGDPDSGIAPGTAFEDIPEDWVCPVCAV
                     GKDMFEPEE"
     gene            complement(4257..4721)
                     /locus_tag="PK442_02060"
     CDS             complement(4257..4721)
                     /locus_tag="PK442_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86462.1"
                     /translation="MRRNSCCSVLILFVLLCGGPALAAVSLPDIPGYTAGALRSTPLS
                     APSGDFGVWLSRTYRSEGRRLLVATLMSGPGAGPLVTGPEGTKTDDRPLGFGSTYEVF
                     SLGGKPAVYETIPSVGVSLAVAVDANSTLTLESSSLGREALEEAALCMMRAM"
     gene            4942..>5142
                     /locus_tag="PK442_02065"
     CDS             4942..>5142
                     /locus_tag="PK442_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOO86463.1"
                     /translation="MHRFALKIYGCQMNVYDGDKIRTALVGRGWEESSEGEADLVILN
                     GCSIRDKAEQKVWSDLGRFAVRW"
BASE COUNT         1083 a         1379 c         1589 g         1091 t
ORIGIN      
        1 actgaagcag gcctcggaaa gcgcagtacc cacagttatc ctctgccgga cagtcatggg
       61 caagggcgtt cctttcatgg aaaacagctc cgaattccac gggaaagcgc ctctcaacga
      121 tctctatgtc caagcgatgc gcgatctcgg aggcgatccg tcgttgctcg aggacgcgag
      181 gaacagaaga acttcagccc agcttccaac ggggaggcat atttcgactt cctccttcgg
      241 tctggatacg ggcgtacctc gcatctatac cgccgacgcg aagaagaccg acaaccggtc
      301 cgccttcggt tccgcgctcg ccgatatcgg aaagctgaac tacaagaagg aaggaggcgc
      361 accgcttctt gtcttcgatt gcgaccttgc cggttcggtg aaaacaggtg aattcgcctc
      421 cctctgcccg gagtggttca tccagacagg catccaagaa cacgcaacgg caacggttgc
      481 aggggcggcc tctacagccg gagttgcggc cctctgggcc gatttcggcg tcttcggact
      541 ttccgagacc tacaaccagc agcgcctgaa cgacatcaac aaggcaaatc tgaaaacagt
      601 cctcacacat gtcggacttg acgtcggcga agacggcatg acgcaccagg cgatagatta
      661 cgtcagtttg cttcggaaca ccttcggctg gaagctcgcg gttccgtgcg atccgaacca
      721 gaccgaccgc gcgacacgct gggcaatcaa ggaaccgggg tgcgtctgtc tcgccatggg
      781 gcgaagcaag ctcgatgtcc tgacgagcgc caagggggcg cccctcttcg gggaggggta
      841 tgaattccgc tacgggaaag cggagctggt atgcgaaggg aacgacggcg ctctcttcgc
      901 tctcggcgcc atgaccggaa gagcgttgaa agccgcgcag atgctgaagg aaaaaggaat
      961 taccgtatcg gtttacgcag tctcctcacc tttgcttccc gacgacgacg cacttcgcgc
     1021 cgcggctgcc acgggaagaa tcatgacgtg cgaagatcac tgcgttgcaa gcggcatggg
     1081 ctctatcctc gcggcaagga tgacggaact tcgtctgaat gcagcattta aagcgcttgg
     1141 tgtgcatcgc tacggagatt ccggcgcttc ggaggacgtc ttcgccgcga tgggactcga
     1201 tgcggcgtcg cttgccgcgg ctttcgagaa gcttctgtag gagctttttg ccgtgagcgc
     1261 acagaaagta ttgctccacg tatgctgcgc tccggatgct accgtaccgt gggcggatct
     1321 ccccggcgaa gggtacgaag tcgttccgta ctggtacggc aataatatac acccggccgc
     1381 agaggagaaa cgaaggcgcg atacgctggc ggcgctctct ctccgtatgg gcggtagtgt
     1441 tctttgggag gagtatgtgc cggaaacgtg gctggaagcg gcatcgcgcc tggcgaacga
     1501 accggaaggg ggcgcgcgct gcgccctttg tttccgcctt caactggaag cggcagccag
     1561 gagagcttca gcaatgaaga ttcctctgtt gtgcacaacc ctgacgataa gtccgcacaa
     1621 gaacgccgaa ctgatcaact ccataggagc ggagtgtgct tcgcagtacg gagttgcgtg
     1681 gctcgcccgt gtcttcagaa agaaaaacgg ttttgtccgc tccgtggcgc tgtcaaagga
     1741 atatggcctg tacaggcagt cgtactgcgg ctgcctgtac agcatgacga gaggtggaaa
     1801 caatcatgga acagcagctc gtggggaagc ttcccccgtc gctccttgaa gggagcgttt
     1861 ttcgtttttg cggcgcgccg cgccctgaag tcatcatcgg ccccggaatc ggggaggacg
     1921 cggcgcttct tcagttgccg gaagggaaat ttctcgccgt agcatccgat cccatcgtgg
     1981 gcgccaggga gggagcgggg aaatttctgg tgcatatcaa cgcgaacgat atcgcctgca
     2041 agggcgggga cccctctttt ttcgtggcga cgcttcttgt tccttccgcg gacggcgttc
     2101 ccatggtaca ggcgcttatg cgggagattc acgaagcctg tctctccatc ggcgcggccg
     2161 taataggagg gcacaccgag ctgacttcac ggtatgatca gccggtcctc gtcggcacga
     2221 tgatgggccc gacggctttt gtacacaggg caaccgatat acgtcccgga gatgttctca
     2281 tcctcaccaa gcacgcgggg ctggagggga tggctattct ggccaacgac cgtccggacc
     2341 ttctgctccc tgccataggt cccgaagggg tggaggaggc gcgcgggtgg ctggaacaga
     2401 tctcggtcct cgaagaggcg aagcttctcc ggggcatggc gcgttttctc cacgacccga
     2461 cagagggagg agtcggaggc ggaatagcgg agatcgttcg cttgagcggt cttggagttg
     2521 aactcgatta cgggagcgtg ccgttctctc ctcttacgaa gaaggctgta cagacgctcg
     2581 atttcgatcc gctgcatctt atctcttccg gagtccttct ggcggttgtt ccgcaggggg
     2641 gggaagccga cgcgctcgcg gctcttcgtg aaaaaggtat ttccggcgtc gcggccggac
     2701 attttgtcga aggcgcgggc aactgtccgg acgatgtccg cgaagagctc tgggggttgt
     2761 tggagaggcc gaaggagcgg agggaatgag tttcgatcat atttcacgcc gtcttcagag
     2821 gctgcgaagc gtcgtggccg aggagggtat ggatgtcttc gttctcctgg ttaccgaagg
     2881 ctataacagc gaatcggccg cctacatctc cggttttcgg gggagcagct gcgcggtcgt
     2941 gatatccgcg gacagggcgc ttttagttac tgacggaagg tatgctcttc aggcgaaggc
     3001 gcagtccccg ttttcagtcg ttttgcagga acagaatacc cttcaggaga aaacagcgga
     3061 actgctgcgc gggggagggt ggacggtcgc cggttttgaa ggggagcggc tctccgtctc
     3121 tctctttcgg gcgtttgaaa aatccgtccc gcactggaaa gactgctctg ttctcattcc
     3181 ttcgctccga agacgaaagg atgaattgga gattgccgca atcgccaagg cggcggacat
     3241 agcctctgct tcctatgaag aagtcctcgg cctcgttcgc gagggaatgt ccgagaccga
     3301 gctgaacgcg cttctcgaat acgccgtcag aaaaagaggc gcggaggcgg ggtggaaggg
     3361 gagcggcttt atcgtcgctt ccggagagcg aagcgcgctc cctcatggaa aggcgacgga
     3421 caggacgttt cgaaaaggag acatagtcac cgtcgatttc ggcgctacgg tggacggata
     3481 catgtccgac cttacccgga acttttcgct gggcccgctg tcagagggag cgcgggagat
     3541 tgaatccgtg cttctggagg cggcggataa agcggcggag gccttgaagc ccggagcgga
     3601 agggaagttc gtcgactccg tagcccgcgg aataatcgct tcaaaagggt ggggcaaaca
     3661 cttctctcac ggactggggc acggactcgg gttggagatc catgaagcgc cgcgtctttc
     3721 acccgtttcg aaggatattc tccgtgaagg ggatatcgtg acaatcgagc ccggtatcta
     3781 catcgaaggg tggggaggta tgcgcatcga ggacgactat ctcgtgacct cgagcggttc
     3841 ggtctgtatc tccggaggga aaggccggaa gactgcggtt gtatccgtct gattccactg
     3901 gagcgtcagg ggattgtcaa cagaaggaac agggatttcg gcaacaggca gttgcctttt
     3961 tctctgcgcc cgatataatg aagttgtatc tgattccgaa ttgatgaagg gggaaatatc
     4021 atgaagaagt acgtgtgcac cgtatgcggt tacgtttacg atcccgccga gggcgatccc
     4081 gattccggaa ttgctccggg gacggccttt gaagatatcc ccgaagattg ggtctgccct
     4141 gtctgcgctg tggggaagga tatgttcgag cctgaagagt agtcttgcgt gtgtgatcac
     4201 gaagcagaaa aagggctcct ttacgggagc ccttctttat tgtgaaaagc agctgattac
     4261 atggcgcgca tcatacacag cgcagcttct tccaacgctt ccctgcccag ggaggaagac
     4321 tccagcgtga gcgtgctgtt tgcgtccaca gcaacggcaa gagagacgcc gactgaaggg
     4381 attgtttcat atactgccgg tttccctcca aggctgaaca cttcatatgt cgagccgaag
     4441 cccagagggc ggtcgtccgt ttttgtcccc tctggtccgg tcacaagagg acccgctccg
     4501 ggaccagaca tcagcgtcgc tacaaggagc ctccgcccct cagaccggta ggttcgggag
     4561 agccacacgc cgaaatcacc cgacggcgca gataaaggcg tactccgcaa cgccccggcc
     4621 gtataccccg ggatatccgg aagtgaaacg gcggcgaggg ccggccctcc gcagaggagt
     4681 acaaacagta tcagcacact acagcaactg tttcttcgca tatcgcaacc tccttgattc
     4741 tctgttcaaa acccgggtac cattgtactc tatcgaaacg gtttttcctt gcgcgtttct
     4801 tccgttcttc ttccttggtg aaaaagagga tgcgtgaacg tacggattga gtttttgacg
     4861 taccaaaatt ccctttgaag ggtatataat actatttctg ttgtttttac ctctattttc
     4921 gcatcgggag gagttttttc tttgcaccgc tttgcactga agatttatgg atgccagatg
     4981 aacgtttacg acggcgacaa gatacgaact gctctcgtcg gtcgagggtg ggaagaaagt
     5041 tccgagggcg aagcggatct cgtgattctc aacggatgca gcattcgcga caaggcggaa
     5101 cagaaagtat ggagcgacct cggccgtttc gctgtccgtt gg
//
LOCUS       DAOSWW010000058         5123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10895_length_5123_cov_3.877466, whole genome shotgun sequence.
ACCESSION   DAOSWW010000058 DAOSWW010000000
VERSION     DAOSWW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10895_length_5123_cov_3.877466"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /gene="hypE"
                     /locus_tag="PK442_02070"
     CDS             <1..973
                     /gene="hypE"
                     /locus_tag="PK442_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOO86464.1"
                     /translation="HGSGGRQSHELVEMLLEGFRKRSIGPELEDAALLPGGYAMTVDG
                     FTVSPRKFPGGDIGHLCVCGCANDLAVRGASPDFLGIGLIIEEGFPSGELAEYMESAA
                     ATCESLGMTLAAGDTKVVPSGAVDGVYFSGSAFGRVHTVHPLSMSNIREGDSLIVSTS
                     IGRHGAAIAAARFNLSVDGLESDCAALWPLIKPLLSFEGLRCMRDCTRGGLGTVLCEW
                     AESTNLHISLDEASLPVDDRVRSLCDVLGFDPLYMACEGCIVLAVSADSARGILDTLR
                     NSPFCADAAIIGTVTRGEPKGMVSMRTSIGGERLVDMPFGELLPRIC"
     gene            complement(1065..1844)
                     /locus_tag="PK442_02075"
     CDS             complement(1065..1844)
                     /locus_tag="PK442_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOO86465.1"
                     /translation="MKILFIGDIMGSPGRKAVAAFLPQLSEREGGFDFVLANCENAAA
                     GKGITQKIVNELFAMGISGMTSGNHIWDKSEGVPVLAAESRIVRPANYPDVCPGQGAM
                     LLENGERRLMVANLQGRVFMPPLDCPFRKADELVAAAGDVPLVIDFHAEATSEKKALG
                     YYLDGRVAALLGTHTHVQTADEEILPQGTAYITDAGMTGGHRSSIGMKLEGVLPRYLT
                     GMPSKFEVSGDGLAFNGVVITLDDETGRAVGITRINERLSH"
     gene            complement(1850..3391)
                     /gene="rny"
                     /locus_tag="PK442_02080"
     CDS             complement(1850..3391)
                     /gene="rny"
                     /locus_tag="PK442_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOO86466.1"
                     /translation="MNFFTAIVALVVGVAAGIGAGFLLSKSFGIKNIENAKAQARAIL
                     REAAMAAEQGKREKLTEAKEEIFKLRQEVEREGKERRSELQRAERRLEQKEENLDKKL
                     DRVAHREEELKAKQEELENRMLELDEIKKKQLERLEEIARMTSEEARELLLRQTEEDA
                     QHFLGLRIKELEEKTKREADRKAREIIVTAVQRCAVEHTSESAVSVVHLPSDEMKGRI
                     IGREGRNIRTFETLAGVDLIVDDTPEAVTLSSFDPVRREIARLSLERLVTDGRIHPAR
                     IEELIEKATKDVENQIMEAGESALLETGVKNMHPELVRTLGQLKFRFSYGQNALQHSL
                     EVAYVSGMIAAELGIDEEIARRAGLLHDLGKAVDHQVEGPHALIGADLARRFNESPEV
                     VSAIASHHEDQEAKSIYDVIVASADAVSASRPGARRESLDAYVKRLEKLEELAKTFNG
                     VSKAYAIQAGREIRVVVTPNITDDAIVHKIAYDIARKIEEEMKYPGQIKVTATKETRA
                     TEYAK"
     gene            complement(3486..3944)
                     /locus_tag="PK442_02085"
     CDS             complement(3486..3944)
                     /locus_tag="PK442_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOO86467.1"
                     /translation="MSKQPERKKIPGAKNPEAYLHSLLSNGVWTKAVVLRKLQGRGIP
                     EEEAEKLIDKYEEAGFFDDGAYALLFVESHPEWGVYRLRDELRRRGVAQEHILKALEG
                     KDEDENAAALAGEWIRMGIDRRKIGERLLRRGFSGNACRNALERACEGEL"
     gene            complement(3928..>5123)
                     /locus_tag="PK442_02090"
     CDS             complement(3928..>5123)
                     /locus_tag="PK442_02090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOO86468.1"
                     /translation="GDVGSGKTVVALIALLAALDGECQGALLAPTEILARQHYLRLSP
                     VFSALGIPCALLTGSLGVKEKREVYAGLESGEIAVVIGTHALFSEGVSFARLGLAIVD
                     EQHRFGVLQKHTFRAKGSAPHVLVMTATPIPRTLTLTVYGDLSVSVISGMPPGRKGVK
                     TSAVSPKKLPAVLRFLKERFHLGERAYWICPLIEESESDLSAATARFEELSAEFGAYG
                     VGLLHGRLPLAEKERAMEDFRSGVTRLLVSTTVVEVGVDVPEATVMVVEDAVRFGLSQ
                     LHQLRGRIGRGNAEGFCILVCTPSTPESRQRIEAFCSSDDGFAIAEADLRLRGPGEVC
                     GVRQSGVTDFRIADLLKDQEILRLARAEAFALVGEDPELRNTPLLADRVFHVLGRSLN
                     LVETA"
BASE COUNT         1014 a         1579 c         1329 g         1201 t
ORIGIN      
        1 gcacggaagc ggaggcagac agagtcacga acttgtggaa atgctgcttg aaggtttccg
       61 gaaacggagt atcgggccgg aattggagga cgcagccctt cttccgggag gctacgctat
      121 gacggtcgac gggttcacag tctccccccg aaagtttccc ggcggagata tcgggcatct
      181 ctgcgtctgc ggctgtgcga acgaccttgc agtccggggc gcttctccgg acttcctcgg
      241 aataggactg attatcgagg aggggtttcc ttccggggag cttgcggaat acatggagag
      301 cgcggccgct acctgcgaat ccctcggaat gacccttgcc gcaggcgata ccaaagtcgt
      361 tccttccgga gccgtagacg gcgtctactt ctccgggagc gctttcggga gagtacacac
      421 ggtccatcct ctcagcatgt cgaatatccg ggagggggac tcgctgatcg tttcgacctc
      481 catcggccgc cacggcgccg cgatcgccgc ggccaggttc aacctctccg tcgacggact
      541 cgaaagcgac tgtgcagcgc tctggccgct catcaaaccg ctcttgtcat tcgaaggcct
      601 tcgctgcatg agagactgca cccgcggagg actgggaacc gttctctgcg aatgggcgga
      661 atcgaccaac ctgcatatct cgctggacga agcgtctctt cctgttgacg acagggtacg
      721 ctctttatgc gacgttttag gattcgaccc gttatacatg gcgtgcgagg ggtgcatcgt
      781 cctcgccgtc tccgcagact ccgcccgagg gattctcgac acccttcgga actcaccgtt
      841 ttgcgccgac gccgcgatta tcgggaccgt cacccgagga gaaccgaagg gaatggtttc
      901 catgaggacc tctatcggag gggagcgcct tgttgacatg ccgttcgggg agctgcttcc
      961 cagaatttgc tgaagggatt tccggcccgg aagcttcaag gacaaagacg aaagagggag
     1021 gcgcatatgc gcctccctct ttcgtctttg tccttcagtc gttttcagtg agacagtctt
     1081 tcgttgattc tcgttatgcc gaccgctctt ccggtttcat catcgagcgt aataacaact
     1141 ccgttgaatg caaggccgtc cccggaaact tcaaacttgg aaggcatccc cgtcaggtac
     1201 ctcggaagaa cgccttcgag cttcatgccg atggagcttc tgtgcccgcc ggtcataccg
     1261 gcgtccgtga tgtatgcagt tccctgcgga agtatctcct cgtcggcggt ctggacgtgc
     1321 gtatgcgttc ccaagagggc tgcgactctc ccatccagat agtacccgag cgcttttttt
     1381 tcagaagtcg cctccgcgtg gaaatcaatc acgagcggaa catcgcccgc cgcggccaca
     1441 agttcatccg ctttgcgaaa ggggcaatcg aggggaggca taaaaaccct tccctgcagg
     1501 ttcgccacca tcaggcgtcg ttctccgttc tcaagcagca tcgccccctg gcccggacat
     1561 acatccgggt agttcgccgg gcggacgatc ctcgattccg cggccagcac cggaacgcct
     1621 tcgctcttgt cccagatatg attgcctgaa gtcattccgg agatccccat ggcgaacagc
     1681 tcattgacaa ttttctgggt aatgcctttc ccggcggcgg cattttcgca gttggcgagg
     1741 acaaaatcaa atcctccctc gcgctccgac agctgcggaa gaaaggcggc gacagccttt
     1801 cttcccgggc ttcccatgat atcgccgata aaaagaatct tcatagcggt tacttcgcgt
     1861 actccgtggc gcgcgtctcc ttcgtcgctg taacttttat ctggccggga tacttcatct
     1921 cttcttcaat ctttcgggca atatcgtacg caattttatg cacgatcgca tcgtcggtaa
     1981 tattcggggt caccaccacg cgaatttcgc gcccggcctg gattgcgtac gccttgctga
     2041 ctccgttgaa cgtctttgcg agttcctcga gtttttcaag acgtttgaca taggcgtcga
     2101 ggctttcgcg gcgcgccccg ggacgcgatg cgcttactgc gtccgcggag gcgacgatga
     2161 catcgtagat gcttttcgct tcctgatctt cgtgatgcga agcgatagcg cttacgacct
     2221 cgggactttc gttgaaccgt cgcgcaagat ccgcgccgat cagagcgtgg ggaccttcta
     2281 cctgatgatc cacggccttg cccagatcat ggagcagacc agcccgacgg gcgatttcct
     2341 cgtcaatgcc cagttcagcg gcgatcattc cggacacgta cgccacttca aggctgtgct
     2401 gaagcgcatt ttgcccgtag ctgaaccgga atttgagctg accgagagtt cgtaccagct
     2461 ccgggtgcat atttttgacg ccggtctcca gcagagcgct ctcgccggcc tccataatct
     2521 gattctccac atccttggtg gccttctcga taagctcctc gatcctggcc ggatgtattc
     2581 ttccgtcggt cacgagacgt tccagcgaca gtctggcgat ctcgcggcgc acagggtcga
     2641 agctgctcag cgtaacggct tccggtgtgt cgtcgacgat aaggtctact cccgccaggg
     2701 tttcgaaggt acggatgttc cgtccctctc tgccgataat tcttcccttc atctcgtccg
     2761 agggaagatg caccacgctc actgccgatt ccgatgtgtg ctcgactgca cagcgctgta
     2821 ccgccgtcac gataatctca cgcgcttttc tgtccgcctc gcgctttgtt ttctcttcaa
     2881 gttccttgat acgaagaccg aggaaatgct gcgcatcctc ctccgtttgg cgaagaagta
     2941 attcccttgc ttcctcgctc gtcatgcggg cgatttcttc gaggcgttcc agctgtttct
     3001 ttttgatttc gtcgagttcg agcatcctgt tctccagctc ttcctgcttt gccttcaact
     3061 cctcttcacg gtgggcaacg cggtccagct ttttatccag gttctcctct ttctgctcca
     3121 aacgacgttc ggcgcgttga agttccgaac ggcgttcctt accttcgcgt tcgacctcct
     3181 ggcgaagttt gaagatctct tctttcgcct cggtgagttt ctcgcgtttc ccctgctcgg
     3241 ccgccatggc ggcttcccgc aaaatagccc gagcctgcgc ctttgcattt tcgatgttct
     3301 tgattccgaa agatttggaa agcaggaacc ccgctccaat tccagcggcg actcctacaa
     3361 cgagcgccac aattgcagta aaaaagttca tagtatcatg atcactcctc gattatattt
     3421 tcaaggtgcc tttgtactcc acgccatgga aggcactctc tcccatgacc tctttatttt
     3481 acaggtcaaa gttccccctc acaagccctt tcaagagcat ttctgcatgc attccccgaa
     3541 aaaccgcgcc gaagcagtct ctcgcctatc tttcgccgat ctattcccat ccggatccat
     3601 tcgccggcaa gcgcagccgc attctcatcc tcatcttttc cttcaagggc cttgagtata
     3661 tgctcctgcg caacgcctct cctccgcaac tcgtcccgaa gtctgtatac tccccattcc
     3721 ggatgcgatt ccacaaaaag aagggcgtag gcgccgtcat cgaaaaaacc ggcttcctcg
     3781 tacttgtcta tgagcttttc cgcttcttcc tccggaatcc ccctgccttg aagcttgcga
     3841 agaacgacag ccttcgtcca gacgccgttt gaaagcagcg aatgcagata tgcctccggg
     3901 ttctttgcgc cgggtatttt ttttctttca ggctgtttcg acaagattca gcgacctccc
     3961 gaggacatga aatacgcgat ccgccagaag cggcgtattc ctgagttccg gatcctcccc
     4021 gacaagagcg aatgcttccg ctcgcgcaag ccggaggatc tcctggtctt ttaaaagatc
     4081 ggcaatacga aaatccgtca caccggactg gcggacgccg cagacttccc cgggaccacg
     4141 aagccggaga tccgcctccg caatagcaaa gccgtcatcc gaggagcaga acgcctcgat
     4201 tctctgcctg ctttccggag tcgacggcgt acacacaaga atgcagaatc cctccgcatt
     4261 tcctctgccg attctacctc tcagctgatg aagctgggaa agcccgaatc ggaccgcgtc
     4321 ctcgacaacc atcactgtcg cctcgggaac atccacccca acctcgacca ccgtggtaga
     4381 aacaagaagc cgtgtgacgc cgctccgaaa atcctccatc gcccgttcct tctccgcaag
     4441 cgggagtctt ccgtggagca gccccactcc gtaggctccg aattccgcgg agagctcttc
     4501 gaacctcgcc gtcgccgcgg aaagatcgga ttcgctctct tcgatgagag ggcatatcca
     4561 gtacgcgcgc tctcccaagt gaaatcgttc ttttaaaaaa cgcagaactg cgggcagttt
     4621 tttcggcgaa accgcactcg tcttcactcc ttttcttccg ggcggcatac ctgagatcac
     4681 ggatacagag agatctccgt agacggtcag cgtcagcgtg cgaggaatcg gcgtcgccgt
     4741 catcactaaa acgtgcggcg cgcttccctt ggcgcggaag gtatgcttct ggaggacgcc
     4801 gaaacggtgc tgttcgtcga cgatggcaag gcccaatcga gcaaacgata ctccttccga
     4861 gaagagagcg tgcgtcccta taacaacggc tatttcgccg gattcaagcc cggcgtacac
     4921 ctctcttttt tctttaacgc cgagagagcc ggtgagcaag gcgcacggaa ttccaagagc
     4981 agaaaaaaca ggggaaagac ggaggtagtg ctggcgggca agaatctcgg tcggggccag
     5041 aagagctccc tggcactctc cgtcgagcgc agcgagcaat gcgataagtg caacaacagt
     5101 cttacccgat cctacatctc cct
//
LOCUS       DAOSWW010000059         5122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10898_length_5122_cov_3.756266, whole genome shotgun sequence.
ACCESSION   DAOSWW010000059 DAOSWW010000000
VERSION     DAOSWW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10898_length_5122_cov_3.756266"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK442_02095"
     CDS             <1..250
                     /locus_tag="PK442_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOO86469.1"
                     /translation="LCAALASAVRDIPLPGNCVFLGEVGLAGEIRPVVRLARRLQEAA
                     RLGFTQAIVSAREGGGGKAPLDVVRVSTLKDALKAVIS"
     gene            247..1608
                     /locus_tag="PK442_02100"
     CDS             247..1608
                     /locus_tag="PK442_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOO86470.1"
                     /translation="MNNCLKKSAMFLVLLFALCACPAFSAQAGTVPSVEGKVFSAALD
                     GTVGVSMENFVAAVLTRAEEEKGGLVIFRLDTPGGLVSSMRAITAAILESSVPVVVWV
                     SPQGARAASAGAFILQAAHVAAMAPGTNVGAAHPVMASGQDAPDKEMKRKITNDLAAQ
                     IRSLAQLRKRNADLAERMVTHSISLTAEEALAEGAADLSAPDLESLLLGVRGRRVLVR
                     GEEKELVPDIGTPVEITMTSREKALQFISSPDIAYLLLTAGILMIVLEVMSPGGFVLG
                     TAGAVMVLLGSFGLRMLPFNWAGIVLLVAGIGVLVLDLMVGGIGILSLFGLASLVTGG
                     LILFRAPGGELLNVSMSVIGGMSLALGLFFLGALFLVVRSMNKKAVSGREGMKGKKAV
                     VVEDLLPEGLVRCHGEIWKARSSSGAFFSKGDEVLVTELDGITLIVRGETGKELPGEE
                     KEE"
     gene            1612..2427
                     /locus_tag="PK442_02105"
     CDS             1612..2427
                     /locus_tag="PK442_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOO86471.1"
                     /translation="MVVDLLELLLSLGTSFGFILIVLFILASAVKIVPEYQRVVVFRV
                     GRLVGAKGPGIVLVIPIIDKVVRVDLRVVTLDVPVQEVITRDNVPIKVNAVVYFRVMD
                     PAFSVVEVENYILATSLLSQTTLRSVVGGADLDEVLSSREKINSELQKIIDERTDSWG
                     IKVSAVELKELELPESMKRAMAKQAEAERERRAKIINAEGEFQAAQKLSEAAKQMELS
                     PITLQLRYLQTLREVSGGDKSSTTIFPLPLDLVKPFMEMFFDKDKKKAGGGDA"
     gene            2675..>5122
                     /gene="leuS"
                     /locus_tag="PK442_02110"
     CDS             2675..>5122
                     /gene="leuS"
                     /locus_tag="PK442_02110"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOO86472.1"
                     /translation="MGYDFQSIEKKWQHTWESEGTFNADADPARKKFYCLEMFPYPSG
                     ALHMGHLRNYSIGDMMTRFLWKRGFNVLHPIGFDAFGLPAENAAMKYKTPANKWTWEN
                     IEHMTEQLKHMGCSYDWRRRVETCNPDYYKWTQWIFLQFFKKGLAYRKNAPVNWCESC
                     NTVLANEQVINDGHCWRCNTPVVKRNLEQWFLKITDYAQELLDDLDNLPGWPDRVKTM
                     QRNWIGRSEGARLTFTEKSTGEIIETFTTRFDTIYGVTFLALAPEHPFVEKMCALSPK
                     GDEIRAFVQKCVTQSAIERTAVGGDKEGFFTGFSAVNPVTGESVPILIANYILMDYGT
                     GAIMGVPAHDQRDFEFARKYDIPVKVVIQPNDESLDGVTMEHAFEDDGITCNSGSFDG
                     LPTAEAIPAMIEWGVEQGFCSREVNFRLRDWLISRQRYWGAPIPVVYCDCCGIVPVPE
                     DELPVLLPAEVKIVEGGKSPLTEDKEWLSVPCPKCGKPGRREADTMDTFICSSWYFFR
                     YCSAREDGAVFNVDEANYWMAVDQYIGGIEHACLHLIYARFFTKFFADLGLCNAREPF
                     TNLLTQGMVIKDGAKMSKSLGNVVDPDEIINTYGADTARLFILFAAPPANDLDWSERG
                     VEGANRFLNRVWRLVEENIALLSSVRERPSLPMERLSEQRMRDLKRKIHTTIRDVTRD
                     IADEKQFNTAVARLMELLNALSSFRPETDDESALFREGVDVLLACLNPFCPHITEELW
                     SLGGHKETLSKLPWPEVEPSALEMDSVTVVVQFNGKVREKEDFPAGLSKEELQARVFA
                     LPSVQKRLEGVEVVRVIV"
BASE COUNT         1049 a         1405 c         1611 g         1057 t
ORIGIN      
        1 gctctgcgcg gcgcttgcgt ccgcggtgcg ggacattccg cttcccggaa actgcgtctt
       61 tctgggggag gtcgggctcg cgggggagat ccgtccggtg gtcaggctgg ccaggcgctt
      121 gcaggaagct gcgcggctgg gttttacaca ggcgatcgtc agcgcccgcg aaggaggagg
      181 cggaaaagcc cctcttgatg ttgtccgggt ttcgacgctc aaggatgctt taaaggcggt
      241 gatatcatga ataactgttt aaaaaagagc gccatgttcc tggttttgct gtttgcactg
      301 tgtgcgtgtc cggcgttttc tgcgcaggct ggtacggttc cgtccgtgga aggcaaggtg
      361 ttttccgcgg cgctcgacgg aacggtgggc gtgtcgatgg aaaattttgt ggccgcggtg
      421 cttacgcgtg cagaggagga aaaaggcgga ctcgttattt ttcgcctgga tactccggga
      481 ggcctggtca gctcgatgag ggccattacc gcggcgatac tcgaatcgtc tgttcccgtc
      541 gtggtctggg tctctccgca gggagcgagg gccgcctcgg ccggagcctt tattctgcaa
      601 gcggcgcacg tagccgcgat ggcccccgga acgaacgtcg gcgccgccca tcctgtgatg
      661 gcgtcgggac aggacgcgcc ggacaaggaa atgaagcgga agatcaccaa cgatctggcc
      721 gcccagatcc gctccctcgc ccagctcaga aagaggaacg cggatctggc ggagcggatg
      781 gtgacccata gcatttcgct cacagcggag gaagcgctgg ccgagggggc ggcggatctg
      841 tcggctcccg atttggagtc gctgcttctc ggcgttcgcg gaagaagagt gctcgtgagg
      901 ggcgaggaga aggagcttgt tccggatatc gggacgccgg tggaaatcac tatgacgtcg
      961 cgcgagaagg cgctccagtt catctcaagc cccgatatcg cctatctgct gcttacagcg
     1021 ggaattttga tgatcgtgct cgaagtgatg tctccgggag ggtttgtgct gggaacggcc
     1081 ggcgccgtga tggtgcttct ggggtcgttc ggacttcgga tgctgccgtt caactgggcg
     1141 ggtattgtcc tcctggttgc aggaatcggc gtgctggtcc tcgatcttat ggtgggcgga
     1201 atcggtattc tgagcctttt cggccttgct tctcttgtga caggggggct gattctcttt
     1261 cgggctccgg ggggtgagct tttgaacgta tccatgagcg tcataggcgg catgagcctt
     1321 gcactcggtc tcttcttcct cggcgcgctc ttcctcgttg ttcgttcaat gaataagaag
     1381 gctgtctcgg gcagagaagg gatgaaagga aagaaggctg tggtcgtgga ggacctgctc
     1441 cctgaagggc tggtccggtg tcacggagag atctggaagg cccggagctc ttcgggggcc
     1501 tttttctcga agggtgacga ggtgcttgtc acagagctcg acggaattac gctgattgtt
     1561 cgtggagaga ccgggaaaga gcttcccggc gaagaaaagg aggaataaac tatggtagtc
     1621 gatcttttgg agctgttgct cagtttgggg acgtccttcg ggttcattct cattgttctg
     1681 ttcattttgg cgtccgcggt gaagatcgta cccgagtacc agcgcgtggt cgtctttcgg
     1741 gtgggacgcc ttgtcggcgc gaagggaccc ggcatcgtgc tggtgatccc gatcatcgac
     1801 aaggtggtgc gagtggacct ccgcgtggtg acgctcgacg tgccggtgca ggaggtcatc
     1861 acgcgggaca acgtcccgat caaggtcaac gcggtcgtct atttccgcgt catggaccct
     1921 gcgttttccg tggtcgaggt ggagaactat attcttgcga caagccttct ctcgcagacg
     1981 acacttcgtt cggtcgtcgg cggagccgat cttgacgaag tgctctcgtc gagggaaaaa
     2041 atcaacagcg agttgcagaa gatcatcgat gagcggaccg attcatgggg catcaaggtc
     2101 agcgccgtcg aactgaagga gcttgagctg cccgagagca tgaagcgcgc catggcgaag
     2161 caggcggagg cggagcgcga acgccgcgcg aagatcatca acgccgaggg cgagttccag
     2221 gccgcgcaaa aactgagcga ggcggcaaaa cagatggagc tttcacccat cacgctccag
     2281 ctccgctacc tgcaaacgct gcgcgaagtc agcggcggag acaaatcctc cacgaccatc
     2341 ttcccgctgc cgctcgacct ggtgaagccc tttatggaga tgttcttcga caaggataag
     2401 aaaaaagcgg gcggcggcga cgcgtaagcg tcaatagtcg gcaatgggag gcggcaggct
     2461 gccgcacgcg gcctgccccg cccgtaaaca aaacggatag tggaatcagg cgattccggt
     2521 atataatacc tgtacaagac catttcggac gactgttttg aaacggacgc acacggcctt
     2581 tcaaggagag agccttcacg agagaagact ttcttccggg acggtcgttt gcgtctgtcc
     2641 tgcgaacggg cgctgtgatc aaggaggaag ggttatgggc tacgattttc aatccatcga
     2701 gaaaaaatgg cagcatacgt gggagagcga agggacgttc aatgcggacg ccgatccggc
     2761 acggaagaaa ttctactgcc tggagatgtt tccgtacccc agcggcgcgt tgcacatggg
     2821 gcacctgcgg aactactcca taggcgacat gatgacccgt ttcctgtgga aacgggggtt
     2881 caacgtcctc catccgatcg gattcgatgc gttcggtctc ccggccgaga acgccgcgat
     2941 gaagtacaag actcccgcca acaagtggac ctgggagaac atcgaacaca tgacggaaca
     3001 gctcaaacac atggggtgca gctacgactg gcgtcgaaga gtcgagacct gcaaccccga
     3061 ctactacaag tggacccagt ggatcttcct ccagttcttc aagaaggggc tcgcctacag
     3121 aaagaacgcg ccggtgaact ggtgcgagag ctgcaacacg gtgctcgcca acgaacaggt
     3181 catcaacgac gggcactgct ggcgatgcaa cacccctgtc gtcaagcgca acctggagca
     3241 gtggttcctc aaaattaccg actatgcgca ggagctgctt gacgatctgg acaacctgcc
     3301 ggggtggcct gaccgagtga agacaatgca gcgaaactgg atcggccgtt ccgaaggcgc
     3361 ccgcctcacc ttcaccgaga agagtacagg tgaaatcata gaaactttca ccacccgctt
     3421 cgacactatc tacggagtga ccttcctcgc actggcgccg gagcatccgt ttgtcgaaaa
     3481 gatgtgcgcg ctctccccga agggagacga gatcagagcg ttcgtccaga agtgcgtcac
     3541 tcagagcgcc atagagagaa cagccgtcgg cggcgataag gagggttttt tcaccggatt
     3601 ctctgcggta aatcctgtca ccggcgagtc ggttcccata ctgatcgcga actacattct
     3661 gatggactac ggtacaggag ccatcatggg cgttcctgcg cacgaccaga gggacttcga
     3721 gttcgcgcgc aagtacgata ttccggtgaa ggtcgttatc cagccgaacg atgaatctct
     3781 cgacggcgta acgatggagc atgccttcga ggacgacgga atcacctgta actcgggatc
     3841 cttcgacggt cttcccactg cggaggctat ccctgccatg atcgagtggg gcgtcgaaca
     3901 gggcttctgc tcccgtgagg tcaacttccg gcttcgcgac tggcttatct cccgccagcg
     3961 ctactggggc gctcccatcc cagtggtgta ctgcgactgc tgcggcatcg taccggttcc
     4021 ggaggacgag ctccccgtgc tccttccagc agaagtgaaa attgttgaag gcgggaaatc
     4081 gcctctgacc gaagataaag agtggctctc cgttccctgc ccgaagtgcg gcaagcccgg
     4141 caggcgcgaa gcggacacca tggatacgtt catctgttcc tcctggtact tcttccgcta
     4201 ctgttcggcg cgcgaagacg gcgccgtctt caatgtggac gaagcaaact actggatggc
     4261 cgtggaccag tacatcggcg gcatcgagca cgcatgcctc cacctgatct acgcccgctt
     4321 cttcaccaaa ttcttcgcgg atctcggttt gtgcaatgcc cgcgagccgt tcacaaatct
     4381 cctcacgcag gggatggtca tcaaggacgg cgcgaagatg tcgaaatccc tgggcaacgt
     4441 cgtcgacccg gacgagatca tcaataccta cggcgctgat acggcgcggc tctttatcct
     4501 cttcgcggcg cctcctgcaa acgacctcga ctggtcggag cgcggcgttg aaggagcaaa
     4561 ccgcttcctg aacagggtat ggcgtcttgt ggaggaaaat atcgcgctgc tctcttcggt
     4621 gcgggaacgc ccctctcttc cgatggagcg tctttccgaa cagcggatgc gcgacctgaa
     4681 gcggaaaatt cacacgacaa tacgcgacgt cacgcgggac atcgccgacg aaaaacagtt
     4741 caatacggct gtcgcacggc ttatggagct gctcaacgcg ctctcatcct tccgtccgga
     4801 gacggacgac gagagcgcgc tcttccggga gggcgtcgat gttctgctgg cctgcctcaa
     4861 ccccttctgc cctcatataa ccgaggagct gtggtcgctc ggcgggcata aagaaaccct
     4921 gtcaaagctt ccatggcccg aagtggagcc ttcggcgctg gagatggatt ccgttaccgt
     4981 cgtggtgcag ttcaacggca aggtacgcga aaaagaggac ttcccggcgg ggctttcaaa
     5041 agaggagttg caggcgcgtg tttttgctct tccgtcggtg cagaagcggc ttgagggcgt
     5101 cgaagtagtc cgcgtcattg tg
//
LOCUS       DAOSWW010000060         5110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10934_length_5110_cov_4.677151, whole genome shotgun sequence.
ACCESSION   DAOSWW010000060 DAOSWW010000000
VERSION     DAOSWW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10934_length_5110_cov_4.677151"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2860)
                     /locus_tag="PK442_02115"
     CDS             complement(<1..2860)
                     /locus_tag="PK442_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HOO86473.1"
                     /translation="MKKLFIALVVLVALAGLAVATMDFAGDLVVREAGSAVKEYLGAD
                     LAVAGIKGNPVKGFSMQGVSLKKDGQPLFSAKFLDVSLNLASLLSKSPKLSLLSVGGV
                     EIDADRLAAQVAQLKFEGGGGGEIPIETVRFVESVVVSKWARADITKLDLSFKGKEIL
                     ADVDMAVNKVPVKGSTTALLDGENVDIRSLAMNVGKGSVSASGKVAPSLAVSGNLKEL
                     DLKELISFWPVVSAEGFDGAVSLSFRGDGEWNAPALAGDMEYAGKSLLGYPVESVKAK
                     WTLGRDKLSVEGLDARVLGMPLAGGMSLGFAAGKAPVVDLSLSGSGIKLAELKKVYPD
                     IGDVSGEVDKFTINLAGTMETLSGVIEFSAPKLGLMGYSVSESRAQVKLTPETATVSA
                     KSQFEGAPITAQGSINEYMTAPKLNLTINVRSLNLTKIASSFPQLKELALQGGANADV
                     WVKGTAAAPEVTVKVWSEKISAMKEAIESPTVMLSLKGETITISNAAAKWRGAAIAAS
                     GTVEGSEKLNITATLENIQPGAIAPFYPDIAQLKIKGAVTAKAVITGKPAAPKIDLTL
                     SSGSLGLLDSVSFKNLKATTSLVGDPKALEKADIDLNISAASAAAEGIGLSDLALKLK
                     KSGQKITILSAGAKSGAGSVSAAGAVALPAKAGEKGNLDLTVTIAKADLAFLAAAGGL
                     GVPLAGTLDGSVALKGALDAPSLVLKASSPKISVAGMTATDLSAALSGTMKDMKIDDF
                     KAKFGGGSLSAKGGVTLGATPAVTLDISGSDLDLAALVSGMPDAKELGIGGKLNVSFN
                     GRFAGAAGKGQGSISSPAFTVMGLKGSNLSYPIALDGNSISSKGASLSFYGGTLRGGG
                     ALDIKTMKFSHTVELNGVDVNSVAQDFTGGLEGKIGGLAKGSASISGALEPKLAYSGK
                     GSVTIGEGSITGFKAVQLLTALYRSGVRYSSVTAPFRLE"
     gene            complement(2960..3790)
                     /gene="dapF"
                     /locus_tag="PK442_02120"
     CDS             complement(2960..3790)
                     /gene="dapF"
                     /locus_tag="PK442_02120"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOO86474.1"
                     /translation="MIPFVKMQGNGNDFIVLDNRDVRFSSDDLFSMARTYCRRRSSLG
                     ADGILVVENSQEADFTMRLFNADGSEGEMCGNGARCIARYAFEKKIAGNEQSFSTLAG
                     IMKAVVTPPFVDLDMGTVPLSSGWFNRPLSVGARSFDTSFLVVGVPHLVLFLEEDALT
                     REETIETGRELCHTYALFPNGTNVNFVVPNDDTHIASVTYERGVEDLTDSCGTGSTAS
                     AIAWVLRNAAVPSGTVVEVKNPGGVNFVTLSFDHSSEFVAASLKGRALMVAEGALFEN
                     "
     gene            3893..>5110
                     /locus_tag="PK442_02125"
     CDS             3893..>5110
                     /locus_tag="PK442_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOO86475.1"
                     /translation="MNKQEFIRWIEEHRAIVPLDGGMGTLLAEKGWTPPMLPEEMNIH
                     SPEVVAGIHAAYINAGARIIETNTFGGTPIKMAHRGLREKAREINARAARLAKSAAGE
                     RALVAGSVGPLGELLAPFGSLTFDEAYASFRTQMEGLLEGGADFLLIETMIDLREAKA
                     AVLAAKDVNPDGAFVVSFTFDQKGRTVTGTPPEVAARWAAYQGAAGVGANCGVGPEAY
                     ISTVEVLSEHAGLPVFVYANAGLPGNEEYRSPEVFATWGKRLAEAGATVIGGCCGTTP
                     EHIAALVAAVRGIHPKKRAPVRGVPLASRSRLVLAGPGHPFRIIGERINVSRKSPLRD
                     EVERFVWTTLREEARLQTEAGAHVIDVNVGLPTIDQQAAMAAAVSAAGQSTALPLSID
                     SDSALVLESGLDAA"
BASE COUNT         1039 a         1596 c         1432 g         1043 t
ORIGIN      
        1 tctccagtct gaaaggggcg gtcacgctgc tgtagcgcac ccccgaccta tagagcgccg
       61 tcaggagctg caccgctttg aagccggtga tgctcccctc tccgatggtc acgctcccct
      121 ttccggagta cgcaagtttc ggctcgagcg cgccggagat gctcgcggac cccttggcga
      181 gaccgccgat ctttccttcg agaccgcctg tgaaatcctg cgcaacactg ttcacatcca
      241 ccccattgag ctcgacggtg tgcgagaact tcatcgtctt gatgtcgagc gcgccgcctc
      301 ctcgcagggt acctccgtag aaggagagag aggccccctt cgaggagatg gagtttccgt
      361 caagagcgat cgggtacgaa aggttcgagc ccttcaggcc catgacagta aacgccggag
      421 aggagataga gccttgtccc ttgcccgccg ctcctgcaaa acgtccgttg aaggagacgt
      481 taagctttcc gccgatgccc agctccttcg catccggcat cccggaaaca agcgcggcaa
      541 gatcgagatc gcttcccgaa atatcgagcg taacagcggg cgtcgccccc agtgtaacgc
      601 cgcccttcgc agagagagac cctccgccga attttgcctt aaaatcgtcg atcttcatgt
      661 ccttcatagt gcctgaaagc gcggcggaaa ggtccgtcgc agtcattccc gcaacggaga
      721 tctttggaga ggaggccttg agaacaagcg acggcgcgtc gagagcgcct ttgagcgcga
      781 ccgaaccgtc gagcgttccg gcgagaggaa ctcccagccc tccggcggcg gcgaggaacg
      841 ccagatcggc cttcgctatg gtgacggtga gatcaagatt tcctttttct ccggcttttg
      901 ccggcaacgc aaccgccccg gcggcggaga cggaacctgc cccgctcttg gctcctgcag
      961 agaggatcgt gatcttctgc ccggacttct tcagcttcag cgcaaggtcc gaaagaccta
     1021 tgccttccgc tgcggccgat gctgcggaga tgttcagatc gatatccgct ttttcaagcg
     1081 cttttgggtc gccgaccagc gatgtcgtcg ccttcagatt cttgaaggag acgctgtcga
     1141 gaaggccgag gctgccagag gagagcgtca ggtcgatctt cggagcggcg ggtttgcctg
     1201 tgatcacagc ttttgcagtc actgcgccct tgatttttaa ctgggcgatg tccgggtaga
     1261 acggcgcgat ggcgccgggc tggatgtttt cgagcgtcgc ggtgatattc agtttctcgc
     1321 tcccctcgac ggttcccgag gcggcgatcg cagcgccccg ccatttcgcc gcggcgttcg
     1381 atatggtgat tgtctctccc ttgagcgaga gcatcaccgt cggcgattcg atcgcctctt
     1441 tcatcgccga gatcttctcg ctccacacct tgactgtgac ttcaggagcc gcggcagtcc
     1501 ccttaaccca cacgtccgcg ttcgcgcccc cttgaagggc cagctccttc aactgaggga
     1561 aggacgatgc gattttcgtc agattcaagc ttctgacatt tattgtaaga ttgagcttcg
     1621 gcgcggtcat gtattcgttg atcgacccct gagccgttat gggcgcgcct tcgaactgcg
     1681 acttcgcgct cacggtcgcc gtctcgggag tcagcttcac ctgcgcgcgg gactcggaaa
     1741 ccgagtaccc catgagcccc agtttcgggg cgctgaactc gatcacgccc gaaagcgtct
     1801 ccatcgttcc ggcaaggttg atcgtgaatt tgtcgacctc gcccgagacg tctccaatgt
     1861 ccggatagac cttcttcagt tcagcgagct ttatccccga tccggagagc gaaagatcga
     1921 caaccggcgc tttcccggcg gcaaacccca gagacatgcc gcctgcgagc ggcataccga
     1981 gcacccgtgc atccagcccc tcgacggaca gcttgtccct gccgagcgtc cacttcgcct
     2041 tgacgctctc cacaggatac ccgagcagcg attttccggc gtactccata tcccccgcca
     2101 atgccggggc attccactct ccgtctccgc ggaaggagag cgacacagcg ccgtcgaagc
     2161 cttcggcgga aacgacgggc cagaaggaga tcagttcctt cagatcgagt tccttgagat
     2221 tgccgctcac cgcaagagag ggggctactt ttccggaagc cgatactgaa cccttcccga
     2281 cgttcatcgc cagcgagcgg atatcgacgt tttcaccgtc gagaagcgcg gtggtactcc
     2341 ccttgacggg aaccttgttg accgccatat caacgtcggc aagaatctcc tttcccttga
     2401 aggaaaggtc gagtttggtg atatctgctc tggcccactt ggagacgaca acgctctcga
     2461 cgaaacgtac tgtctctatc ggtatctcgc cgccgcctcc gccctcgaac ttcaactgcg
     2521 caacctgggc cgccagacgg tcggcatcga tctcgacgcc tccgacggag agcaggctca
     2581 acttcggcga cttcgagagc aacgacgcca agttgagcga gacatcaagg aatttcgcgc
     2641 tgaagagagg ctgaccgtcc ttcttcagcg atactccctg catcgagaaa cccttgacag
     2701 ggttcccctt gattcccgca accgcgaggt cggcgccgag atactccttt acggcggaac
     2761 ctgcctcccg taccacaaga tcgccggcga aatccattgt cgccactgcc agtccggcga
     2821 gcgctacaag aacaaccaac gcaatgaaca gcttcttcat ggttctctac ctccctttct
     2881 tcgcgtcatt gacgacgcgt cctctacatt cccccatccg aatcgtgata tgcttttctc
     2941 attataacat tctatctctt taattttcaa aaagagcgcc ctccgcaacc atgagggcgc
     3001 gtcccttgag agaagccgct acgaactcgc ttgagtgatc gaagctgagc gtcacgaaat
     3061 tgactcctcc cgggtttttt acctctacga ccgttccgga aggaacagcg gcgttccgaa
     3121 gaacccacgc aatagcggac gctgttgaac ctgtgccgca ggaatcggtg agatcctcca
     3181 cacctctttc gtaggttact gaagctatat gtgtgtcgtc attcggcacg acaaagttaa
     3241 catttgtccc gttgggaaaa agcgcgtagg tatgacataa ctcgcgcccc gtctctattg
     3301 tctcttcccg ggtgagcgcg tcctcttcaa ggaaaaggac aaggtgcgga acgccgacca
     3361 cgaggaagga tgtatcgaac gatctagcgc ctaccgagag cgggcggttg aaccagccgg
     3421 aagaaagagg aacagtcccc atgtccagat cgacgaacgg aggcgttaca accgctttca
     3481 taatacctgc aagagttgaa aacgactgct cgtttcctgc gatcttcttt tcgaaagcat
     3541 atctcgcgat gcagcgggcg ccgtttccgc acatctctcc ctcgctgccg tcggcattga
     3601 agagacgcat cgtaaaatca gcctcctgcg aattttccac aacgagtatc ccgtccgctc
     3661 cgagcgagct tcgtctgcgg caatacgttc gcgccataga aaaaagatca tcagatgaaa
     3721 aacgcacatc cctgttatcc aagacaataa aatcgttgcc gttaccctgc atcttcacaa
     3781 aaggaatcat tgttttccct cctcctcttt tccttctata ttttacacta tccttcttca
     3841 gttctttttg ctatattgat aaacttatac ggagcgcaga gggggaagaa aaatgaacaa
     3901 acaagaattc atccgctgga tcgaagaaca ccgcgcgatt gtgccgcttg acggcggtat
     3961 ggggacgctg ctcgcggaga aggggtggac ccctccgatg ctgccggagg agatgaacat
     4021 tcacagtccg gaagtagtcg ccggcatcca tgccgcctat ataaacgccg gggcgcgcat
     4081 catagagaca aacactttcg gcggaacccc gataaaaatg gcccacagag ggcttaggga
     4141 aaaggcgcgc gagatcaacg cacgggctgc gcgcctcgcg aagagcgcag ccggagagcg
     4201 cgcgctcgtc gccggttccg tcggcccgct tggcgagctt ctggcgccgt tcggctcact
     4261 gaccttcgac gaggcttacg cctctttccg cacgcagatg gaggggcttc tggaaggcgg
     4321 ggcggacttt cttctcatcg aaacgatgat cgacctccgg gaggccaagg cggctgtcct
     4381 tgccgccaag gacgtaaatc ccgacggcgc gtttgtggtg agcttcacgt tcgaccaaaa
     4441 gggcagaacc gtcacgggaa ccccgccgga agttgccgct cgctgggcgg cctatcaggg
     4501 cgccgccgga gtgggcgcca actgcggcgt gggtccggaa gcgtacatct cgacggtaga
     4561 ggtcctgtca gagcacgcgg gccttccggt ctttgtctac gcgaacgcgg gacttccggg
     4621 caacgaagag taccgctccc ccgaggtctt cgcaacatgg gggaaacgcc ttgccgaggc
     4681 cggggcgacg gtgatcggcg gctgttgcgg caccactcct gagcacatcg ccgctctcgt
     4741 tgcggcagta cgcggtatcc acccgaagaa acgcgctccc gtccggggag ttccgctggc
     4801 aagccggagc cgcctcgtgc ttgcggggcc gggacacccc ttccgcatca tcggcgagcg
     4861 gatcaacgtc tcgcgcaaat ccccgctcag ggacgaggtc gaacgcttcg tctggacgac
     4921 gctccgcgag gaggcgcggc tgcagacgga agcgggcgcg catgtcatcg acgtgaacgt
     4981 cgggttgccg acgatcgatc agcaagccgc aatggccgcc gcagtctccg ccgcaggcca
     5041 gtccactgcg ttgccgctct ccatcgacag cgacagcgct ctggtgctcg aatcggggct
     5101 cgacgccgcc
//
LOCUS       DAOSWW010000061         5094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_10981_length_5094_cov_3.725938, whole genome shotgun sequence.
ACCESSION   DAOSWW010000061 DAOSWW010000000
VERSION     DAOSWW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10981_length_5094_cov_3.725938"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PK442_02130"
     CDS             <1..410
                     /locus_tag="PK442_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOO86476.1"
                     /translation="GLEDLLNRQIGGDPDNPAIFAARMRIGFSSGTLPTASLYSLGGS
                     STLRGYDGGEYEGTKMFLANLELRVPIEKAFGLVLFYDTGNAWLEESMNFSDLHDAWG
                     VGVRVKTPLGNFRLDHATGENETRTHFGFGEMF"
     gene            433..1746
                     /locus_tag="PK442_02135"
     CDS             433..1746
                     /locus_tag="PK442_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86477.1"
                     /translation="MHPSAHKGVLNGFFKGCVLFFLAAALGFVIPSRACAYAGDIRVE
                     GVPFWLRPSVERSLRSVWNEIAQRSGFSGEDRRALLSVVAERLFVGYSSSVLLSESGV
                     IVTLSPVSPVAWTVEIKTPSLSSPVEEWFAENVAELGGTLERMLALLPLEALSWADIA
                     LKEAIEEVCAPLLPGWNPSLIVRLEGRGIASAPSTADEKRILQISFSPKPPLVLAIVP
                     SISSSTLPVAFRSDLREKILRTLAPVVGLPIEWAAKNKLRIEALAAEALFDTNTVSNA
                     RATVDVSFSPEQLSPVQAEVESPRYTIRAWIAAYAGADGKYPEIGLHLGRNALPLSGW
                     DVELYGEWILAANDFSLESRWGARWSPWKHILIGAEAAFPGNVLWYRLSVAGGSRSPY
                     FWWRLSEDGDSMVGFGYRLDERISLELHYDERSEDTLTLKAISDL"
     gene            1780..2892
                     /gene="lpxD"
                     /locus_tag="PK442_02140"
     CDS             1780..2892
                     /gene="lpxD"
                     /locus_tag="PK442_02140"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOO86478.1"
                     /translation="MEGVWTLRSLAERVEGRVIGDAALLVEGVASPSKARRGEIVCLW
                     NAFDTASGQKTDSPEPSPEAVNSSEGVLFLAAPEFFARHTDASGVAVEHPKKVFPLVL
                     DLFSRALRSGAQCGIHPTAVVSPKASVAPSAWIGPLCIIEEGAVIEERAFLHGRVFIG
                     RDCCVGAETTVEPGAVLFESVRTGRRCLIHSNAVLGCDGFGITPSAGEAPPEKIPQIG
                     GVVLGDDVELGVCSTVDRGTLDDTVIANGTKVDNHVHIAHNVRIGRNCILVAMTGIAG
                     SAILEDNVIMAAGSGVKEHTRIGKGATVAAKGGAIKDVPPGEVVSGFPARPHRENFRV
                     QAALQRLPETLSRVKKLEKRLDALLPDGSGGSGAGE"
     gene            2889..3758
                     /locus_tag="PK442_02145"
     CDS             2889..3758
                     /locus_tag="PK442_02145"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOO86479.1"
                     /translation="MTAIRRLLHPVCFSGQGLHTGESSEVVISPLPSGSGPELEIEGA
                     RAPLERCPVSGAGRGTTVFLPHSDLRARTTEHLFAALAVLGLWSVRISLKGPEIPAAD
                     GCSALFAESLYGASAPEKPEEDSSMRMDAFSPAFPVTIVDGEKESSLSLFPWGALSIT
                     YVIQYAERPIGTQMATFDAEKDDFRKYIAGARTFALDTEIERLRAAGLARGGSLDNAV
                     VVGDETVQALGGLRFSDEFVRHKILDLLGDLYLLGRPLNARITAIRAGHAMHCILVDR
                     IRRLCRGGRRANQ"
     gene            3784..4266
                     /gene="fabZ"
                     /locus_tag="PK442_02150"
     CDS             3784..4266
                     /gene="fabZ"
                     /locus_tag="PK442_02150"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOO86480.1"
                     /translation="MLDIQKIMKFLPHRFPFLLVDRILEIDEAHAVGIKNVTINEPFF
                     QGHFPSEPVMPGVLVLEAMGQVAAMMLSARPGSEHLITFLTGVEKAKFRRPVKPGDQL
                     VTTAEMLKLRGKMGKVRTVGRVDGAVAAEAEFSFVAAETLAASEEKAAPSRSGEEAAR
                     "
     gene            4267..5088
                     /gene="lpxA"
                     /locus_tag="PK442_02155"
     CDS             4267..5088
                     /gene="lpxA"
                     /locus_tag="PK442_02155"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOO86481.1"
                     /translation="MPLIHPTALVSPKAELADDVVVGPYCIVHDRVRIGSGTVLSSFV
                     TLLDFVETGKNCRFEQHVVLGGEPQDHAFNGEESWVRIGDAVTLRENVTVHRATGEGA
                     CTVVGDESLLMEGVHVGHNAVVGKRATLANKVGLSGFSSVGDGAVMGGMSGIHQFVRV
                     GALCMIGGLSKIVKDIPPYLMADGHPADIYGLNKVGMRRAGFDASARSAVRALYDELF
                     RGGLPFRQAVERLAASEKGNDPVAREILLFCGESKRGVALWTKGRVSKGKDHEGD"
BASE COUNT          986 a         1389 c         1591 g         1128 t
ORIGIN      
        1 aggggctcga agatctcttg aaccgccaaa tcggcggaga cccggataat cctgctattt
       61 tcgccgcgag gatgcgtatc ggcttctctt ccgggacctt gccgaccgcc agtctttatt
      121 cgctcggtgg ttcgagcacg ctcagaggat atgacggcgg ggaatacgag ggaacgaaga
      181 tgtttctcgc gaatctcgaa ctgcgcgtcc cgatcgagaa agcctttgga ctggttctct
      241 tctacgatac cggcaatgca tggcttgaag agagcatgaa cttttccgat cttcatgatg
      301 cgtggggagt cggcgtcagg gtaaagactc ctctggggaa cttccggctc gaccatgcca
      361 cgggagagaa tgaaacacga acgcatttcg gttttggaga aatgttctag agcgcccgaa
      421 ggaggcgccg atgtgcatcc ttctgcgcac aagggagtat tgaacggatt cttcaagggc
      481 tgtgtgctgt ttttccttgc cgcagccctt ggcttcgtta tcccctcccg agcgtgcgca
      541 tacgccgggg atatccgggt cgaaggcgtc ccgttctggc ttcgtccaag cgtcgagagg
      601 agtctgcgtt ctgtatggaa cgagattgcg cagcgctccg gattttccgg agaagatcgc
      661 cgtgcgctcc tctccgtcgt tgcggagagg cttttcgttg gctactcctc ctcggttttg
      721 ctctccgagt ccggtgtgat tgtcaccctt tctccggttt cgcccgttgc gtggacggtg
      781 gagataaaaa cgccgtcgct ttcctccccg gttgaggagt ggtttgctga aaatgtcgcg
      841 gagctcggcg ggacgctgga acggatgctt gcactgcttc cgctcgaggc gctttcttgg
      901 gccgatatcg cattgaagga agcgattgaa gaagtatgcg cccctctcct tcccggctgg
      961 aacccgtcgc tcattgtacg ccttgaaggg aggggaattg caagcgcacc gagtacagcg
     1021 gacgaaaaac gtattctgca gatctctttt tctcccaaac ctcccttggt gctggctatc
     1081 gttccgtcca tctcttcctc cacgctgccc gtagcctttc gctccgacct gagggagaag
     1141 atattgagga cgcttgctcc ggttgtcggt ctgccaatag agtgggcggc aaaaaacaag
     1201 ctccgtatag aagctcttgc cgcagaggcg ctgttcgata ccaatacggt gtcgaacgcc
     1261 cgggcgaccg ttgacgtttc tttctccccc gagcaactct cccccgtgca agcagaagtg
     1321 gagagcccga gatatactat ccgggcgtgg atagccgcgt atgccggagc ggacgggaag
     1381 taccctgaga tcggccttca cctgggaaga aacgcgctgc cgctctccgg gtgggatgta
     1441 gagctctacg gcgaatggat actcgcagcc aacgattttt cgttggaaag cagatgggga
     1501 gcgcgctgga gtccgtggaa gcacatcctc atcggagcgg aagcggcctt tccgggaaat
     1561 gttctctggt atcggctctc ggttgcaggc ggttcccgtt ctccgtattt ctggtggcgt
     1621 ctgagcgagg atggcgattc aatggttggt ttcggatacc gcctcgacga gaggatctcc
     1681 ctggagcttc actatgatga acgttcggaa gatacgctga cgctgaaggc gatcagcgat
     1741 ctgtaaaccg agaaatttgc tttgaacgga ggacatggaa tggaaggagt atggactctc
     1801 cggtctctag cggagagggt cgaaggcagg gtgatcggag atgcggcgct tcttgtggaa
     1861 ggcgtggcct ctccttcgaa ggctcgcagg ggagagatcg tctgtctgtg gaatgcattc
     1921 gatacggctt ccggacaaaa aacggactct ccggagcctt cgcctgaagc ggtcaattcc
     1981 tccgagggcg ttcttttcct ggcagcgccg gagttttttg cccgccacac cgatgcgtcc
     2041 ggagtagccg ttgagcatcc gaagaaagta ttccctcttg tgcttgatct tttttcgcgc
     2101 gctcttcgtt ccggcgctca gtgcggaatc catccgacgg cggttgtgtc tccaaaggcg
     2161 agtgttgctc catccgcctg gataggtcct ctctgtataa ttgaggaagg cgccgtcatc
     2221 gaggaacgcg cttttttaca tggccgggtt tttatcggca gagactgctg tgtgggggcg
     2281 gagaccacag tcgaaccggg ggcggtcctc tttgaaagcg tgcgaacagg acgccgctgc
     2341 ctgattcact ccaatgcggt tctcgggtgc gacggattcg gtattactcc ctccgcaggc
     2401 gaggcgccgc cggagaaaat tcctcagata ggcggcgtcg tcctcggaga cgacgtggag
     2461 ctgggcgtct gctcaacggt ggacagggga acgcttgacg atactgtgat cgcgaatggt
     2521 acgaaggtgg ataatcatgt gcacatcgcg cacaatgtcc gcataggcag gaactgtatt
     2581 ctcgtcgcca tgacgggaat cgccggcagc gccatccttg aggacaacgt gatcatggcg
     2641 gccggcagcg gggtgaaaga gcatacgcgg atagggaagg gcgctacggt cgcggcgaaa
     2701 ggcggagcga tcaaggatgt tcctccgggg gaggtcgtct cgggctttcc ggctcgtcct
     2761 cacagagaga acttcagggt acaggcggcg ttgcagcgcc ttccggaaac gctctcccgt
     2821 gtaaagaagc tcgaaaaacg ccttgacgcg ctgctccccg atgggagcgg cgggagcgga
     2881 gcaggggaat gaccgcgatc cgccgtctgc ttcatccggt ctgcttctcc ggacaagggc
     2941 tccatacagg agaatcgtcg gaggtcgtca tctctccctt accctccggt tccggacctg
     3001 aactggagat agaaggagca agggctcctt tggagcgctg ccccgtttcc ggcgcgggac
     3061 gtggaacgac agttttcctt ccgcacagtg atctacgggc tcgtacgacc gagcatctct
     3121 ttgcagcgct ggccgtgttg gggctctggt cggtgcggat atcgctcaag gggccggaga
     3181 ttcctgctgc ggacggatgc tccgccctct tcgccgaatc gctctacgga gcgtcggccc
     3241 cggaaaaacc ggaagaggat tcgtccatgc gaatggacgc cttttctccg gcgtttcctg
     3301 tgacaatagt tgacggcgag aaggagagct ctctctctct cttcccttgg ggagcgcttt
     3361 cgatcacgta tgtcatacag tacgccgaac ggccgatcgg aacccagatg gcgacgtttg
     3421 acgcagaaaa agacgacttc cggaaataca tcgcaggagc gcggaccttc gcgcttgata
     3481 cggaaataga acgcttgcgc gccgccgggc ttgcccgcgg agggagtctt gacaacgcgg
     3541 tcgtcgtggg agatgaaact gttcaggctt tgggaggctt gcgtttttcc gacgagtttg
     3601 tccggcataa gattctcgac ctgttgggcg acctttatct gctcggacgg cccttgaacg
     3661 cccgaattac agccatccgg gcggggcacg caatgcactg tattcttgtg gacaggattc
     3721 gcaggctctg ccggggcgga cgcagagcaa atcaatgaat tttttatgaa aaggagacga
     3781 actatgcttg atatccagaa gataatgaaa tttttgccgc accgtttccc ctttcttctc
     3841 gtcgaccgta tcctcgaaat cgacgaggct catgcagtgg gtattaaaaa cgtcacgatc
     3901 aacgagccct ttttccaggg gcatttcccc tccgagcctg ttatgcctgg agttctcgtt
     3961 ctggaggcga tgggacaggt tgcggcgatg atgctctccg cccgtccggg aagcgagcac
     4021 ctcattacgt tccttactgg agtggaaaaa gcgaaattcc gcaggcccgt aaagccggga
     4081 gaccagcttg tgacgactgc ggagatgctc aaactcaggg ggaagatggg gaaggttcgc
     4141 accgtaggtc gtgtggacgg agcagttgcg gcggaggcgg aattcagttt tgtggctgcc
     4201 gagaccctcg ccgcctcgga agagaaagcc gccccctcgc gaagcgggga ggaggcggct
     4261 cgttagatgc cgctcatcca tccgacggct cttgtttcac cgaaggcgga actggcggac
     4321 gacgtcgtcg tcggccccta ctgcatagtg catgatcgcg tccggatcgg gtcggggaca
     4381 gtgctctcat ctttcgtcac tctgcttgac ttcgttgaga cggggaaaaa ctgccgtttt
     4441 gaacagcacg tcgtcctcgg cggcgagccg caggatcatg cgttcaatgg agaggagtcg
     4501 tgggttcgca taggcgacgc cgtgacgctc cgtgaaaacg tcacggtgca tcgcgctacg
     4561 ggcgaaggcg cctgcaccgt cgtcggcgac gaatcgctgc tgatggaagg agtccacgtc
     4621 ggacacaatg ccgtcgtcgg gaagcgcgct accctggcga acaaggtcgg cctctccgga
     4681 ttctcctccg tcggcgacgg cgccgtcatg ggcggtatgt ccggtatcca tcagttcgtc
     4741 cgtgtcgggg ctctctgcat gatcggaggg ttgtcgaaga ttgtcaagga tattcctccc
     4801 tacctcatgg cggacggaca ccctgccgac atctacggtc tgaacaaggt ggggatgcgg
     4861 cgtgcgggat tcgacgcgtc cgcccgttcc gcagtccggg ctctctacga tgaactcttc
     4921 agaggaggtc ttcccttccg ccaagccgtc gaacgtctcg ccgcttccga aaagggaaac
     4981 gatccggttg ccagggagat tctcttattc tgcggggaat ccaagcgggg cgttgccctc
     5041 tggactaagg gacgcgtctc caaggggaag gatcatgaag gagattaagc tgct
//
LOCUS       DAOSWW010000062         5058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11100_length_5058_cov_4.028383, whole genome shotgun sequence.
ACCESSION   DAOSWW010000062 DAOSWW010000000
VERSION     DAOSWW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11100_length_5058_cov_4.028383"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PK442_02160"
     CDS             <1..302
                     /locus_tag="PK442_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO86482.1"
                     /translation="ALIVASSGAMLTTVAKNKYAIGYDGIGYVNNTVKPLMVGGKKGS
                     AETALDGTYPIARFLYMFTKGWPTGEIMKFINFVLSDEGQKIVAETGFVPLRVLK"
     gene            417..1280
                     /gene="pstC"
                     /locus_tag="PK442_02165"
     CDS             417..1280
                     /gene="pstC"
                     /locus_tag="PK442_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOO86483.1"
                     /translation="MKDRLPGIVVKGMSSAGIFIMIFILYFLFREGIPVLKAVTLKDL
                     FFGDLWYPAENPPVLGMFPLIVGTLAVTAASSLLALPFSLLIAVFVSEVAPRPVRELL
                     KPVLELLGFFPSIVLGFIGMVVLAPWLQETFDMLSGLNLLNASVLLGVLTVPIVSSLA
                     EEALSAVPADLRDASYALGATRFETTRKVVVPSALPGILSASLLGVMRALGETMVVLM
                     AAGGAAIIPLSIFDPVRPLTSTIAAEMGETPVGSPHYAALFFGGIVLLLMTLGINLLS
                     AWIEKKGKLVQ"
     gene            1281..2135
                     /gene="pstA"
                     /locus_tag="PK442_02170"
     CDS             1281..2135
                     /gene="pstA"
                     /locus_tag="PK442_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOO86484.1"
                     /translation="MDGRRFADMMMTVLSWAAALLLTGMVVAIIGFVVVRGYSVLSLD
                     FLIEPPRDSMTRGGIMTPLVGTLQLVGVAMFFAIPVGVATGLYFAEYARDDMFTSILR
                     LAIRSLAGVPSVVFGLFGLSLFVILLGFGPSLLAAALTLACLTLPLMVTASEQAFLAV
                     PKDYRDASYALGASKWQTIRKVVLPAAAPTVITGIILSVGRVAGETAPIMFTGAAFFA
                     PHIARSLFSEVMALPYHIYVLATAGTFIEETRPIQYGTVLVLLLLVLGICSGGIYMRS
                     RLRRLMRR"
     gene            2152..2910
                     /gene="pstB"
                     /locus_tag="PK442_02175"
     CDS             2152..2910
                     /gene="pstB"
                     /locus_tag="PK442_02175"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOO86485.1"
                     /translation="MEKILVSSRGVNLYYDSARVLKDITFDVMEKQVTALIGPSGCGK
                     SSFLRCINRMNDFIPSARVEGEILVGGSNIYAGGVDVIALRRNVGMVFQKPNPFPMSI
                     YDNVAYGPRLHGVKQKDRLDETVKQSLVGAALWDEVKDKLKTPGTGLSGGQQQRLCIA
                     RAIATEPEILLMDEPTSALDPMATARIEELVRELKKRYTVVIVTHNMQQAARISDRTA
                     FFLMGDLVEYDATPKMFTSPEDKRTEDYITGRFG"
     gene            2939..3661
                     /gene="phoU"
                     /locus_tag="PK442_02180"
     CDS             2939..3661
                     /gene="phoU"
                     /locus_tag="PK442_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOO86486.1"
                     /translation="MEPVSTRKQLEADLTELKRMVLRLGKMAEESISKAIWALKNQDA
                     ELARMVLAKDDDIDDLAEALDNACMHFAARFQPLGEDLRAVTSIMHMAVDLERIGDYG
                     GNIAKVAAELADKELIKPLIDIPRMLDVLSAMMSKALAAFDTNNAEMAKEVFAMDDMM
                     DDLEKQIMRELLLLMMERPQRIEQATQLLNVARTLERGGDHVTNVAERVVYILTGKTV
                     KASAFRRPRPAALNGRKEHEER"
     gene            3667..4356
                     /locus_tag="PK442_02185"
     CDS             3667..4356
                     /locus_tag="PK442_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO86487.1"
                     /translation="MPEARILIVEDEDAIMEIVSQALRRHGYETTSASDGDAALEKIF
                     SLRPDLVILDLMLPKMDGWEVCRRMRAEPETERIPILMLTARRDERDVVEGLELGADD
                     YMKKPFSLAELTARVKALLRRTGERRESRIVENGDLRIELDDETALLRGTPLDLSPTE
                     FRLLEALARKMGRTASREELLSKIWSFYGGDTRTVDVHISRLRKKLDDGKSPALAINS
                     LRGRGYRLVWE"
     gene            4362..>5058
                     /locus_tag="PK442_02190"
     CDS             4362..>5058
                     /locus_tag="PK442_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86488.1"
                     /translation="MFTLKKKIVAVISLAVMLAFGISWLLLYGVVRNGIVRQSTLELS
                     QQTALLAASLKDGGMERFTANVEKWEDILRGRVTLIGANGVVLADTERSPEAMENHLT
                     RPEVKGALASGEGSALRYSATTNMYYLYFARKVEAEGRVAVLRAAYPLEFLSEAFAET
                     RNKFIFYLFVAVVLILAFGTWLARLFFRPLDRIVEFAGKIAQGEAVRFPIMAEPELQR
                     LSSALDGMSARLRG"
BASE COUNT          882 a         1415 c         1787 g          974 t
ORIGIN      
        1 gggccctgat cgtggcctcc tcgggagcca tgctcaccac cgtggccaag aacaagtacg
       61 cgatcggcta cgacggcatc ggctacgtca acaacaccgt gaagcccctg atggtcggag
      121 gcaagaaggg ttccgcggag acggcgctcg acggcacgta ccccatcgcc cgcttcctct
      181 acatgttcac gaagggctgg cccaccggcg aaatcatgaa gttcatcaac ttcgtcctga
      241 gcgacgaggg gcagaagata gtggccgaaa ccggattcgt tcccctgaga gtactgaaat
      301 aacatcgacg actcatccag agagttcctc cagccgtacc ccttcccctc acggggaggg
      361 ggctttttcc gggcccgccg agggcgacga attcgacgaa cgaaagaggt tttttcatga
      421 aagacaggct tccgggaatt gtagtcaagg gaatgtcgag cgcggggatc ttcatcatga
      481 tcttcatcct ctatttcctg ttcagagagg gtattcccgt gctgaaagcg gtgacgctga
      541 aggatctgtt tttcggcgat ctgtggtatc cggcggagaa tcctccggta ctggggatgt
      601 ttcctcttat cgtgggaacg cttgcggtga cggcggcctc ctcgctgctg gcgcttccgt
      661 tcagtctgct gatcgcggtt ttcgtctccg aggtggcccc caggccggtg cgcgagctgc
      721 tgaagccggt gcttgagctg ctgggcttct tcccctccat cgtgcttggg ttcatcggga
      781 tggtggtgct ggcgccctgg ctgcaagaga cgttcgacat gctctcgggg ctgaatctgc
      841 tgaacgcctc ggtattgctc ggggtgctga ccgtgccgat cgtgagttcg ctcgccgagg
      901 aagcgctgag cgcggtgccc gccgatctgc gggacgcctc ctacgcgctc ggcgcgaccc
      961 ggttcgagac gacgcggaag gtggtcgtgc cctcggcgct ccccggtatt ttgagcgcgt
     1021 cgctgcttgg ggtgatgcgc gcgctggggg agacgatggt ggtgctgatg gcggcgggcg
     1081 gcgcggcgat catcccgctc tcgatttttg acccggtgcg tccgctgacc tccaccatcg
     1141 cggcggagat gggggagact cccgtagggt cgccgcacta cgcggcgctc tttttcggcg
     1201 ggattgtcct gctgctgatg acgctcggga tcaatcttct ctccgcgtgg atagagaaga
     1261 agggaaagtt ggtgcagtag atggacgggc gcagatttgc cgacatgatg atgacggtcc
     1321 tctcctgggc ggcggcgctg ctgctgaccg gaatggtggt cgcgatcatc ggcttcgtcg
     1381 tagtgcgggg gtacagcgtg ctctcgctgg actttctgat cgagcctccc cgcgacagca
     1441 tgacgcgagg ggggatcatg acgcccctgg tggggacgtt gcagctggtg ggtgtggcga
     1501 tgtttttcgc gattcccgtg ggggtggcga ccgggctgta tttcgcggag tacgctcgcg
     1561 acgatatgtt cacgtcgatt ctgcggctgg cgattcgctc tcttgcgggc gtgccgtcgg
     1621 tggtgttcgg gctcttcggc ctgtcgctct tcgtgattct gctggggttc ggtccgagcc
     1681 tgctggccgc ggcgctcacc ctcgcctgtc tgacgctgcc gctgatggtg acggcgtcgg
     1741 agcaggcgtt tctggcggtg ccgaaggact accgtgacgc ctcgtacgcc ctcggggcct
     1801 cgaagtggca gaccatccgc aaggtggtgc ttcccgccgc ggcgccgacg gtgatcacgg
     1861 ggatcattct gagcgtcggc agagtggcgg gggagacggc gcccatcatg ttcacggggg
     1921 cggccttctt cgcgccgcac atcgcgcgca gcctcttctc cgaggtgatg gcgctgccgt
     1981 accacatcta cgtccttgcc acggcgggga cgttcatcga ggaaacccgg ccgattcagt
     2041 acggtacagt gctcgtgctg ctgctgctgg ttctgggcat ctgttccgga ggcatctaca
     2101 tgcgttcccg tctccgcagg ctgatgcggc gataggggtg gagggactat aatggaaaaa
     2161 atactggtga gttcacgcgg cgtgaatctg tattacgatt cggcgcgcgt gctgaaggat
     2221 atcacgtttg atgttatgga aaagcaggtc acggcgctca tcgggccgtc ggggtgcggc
     2281 aagagcagct ttttgcgctg tatcaaccgg atgaacgact tcatcccgag cgcgcgggtt
     2341 gagggggaga ttctggtcgg cggttccaac atctacgcgg gcggcgtgga cgtgatcgcg
     2401 ctccgccgga acgtggggat ggtcttccag aagccgaacc ccttcccgat gtcgatctac
     2461 gacaacgtgg cgtacgggcc tcgcctgcac ggcgtcaagc agaaggacag gctggacgag
     2521 accgtgaagc agagccttgt gggcgcggcg ctctgggacg aggtgaagga caagctgaag
     2581 actcccggta ccgggctctc cggcgggcag cagcagcgtc tgtgcatcgc gcgggcgatc
     2641 gcgacggagc cggagattct gctgatggac gaaccgacga gcgcgctcga cccgatggcg
     2701 acggctcgca tcgaagagct ggtccgcgag ctgaaaaagc ggtacactgt ggtgatcgta
     2761 acccacaaca tgcagcaggc cgcccgcatc tcggaccgta ccgccttctt cctgatgggc
     2821 gacctcgttg agtacgacgc gacgcccaag atgttcacgt cgccggagga caagcggacg
     2881 gaggactaca tcacggggcg gttcggctag gaaggccggg agagaggaga cgatgagcat
     2941 ggagcctgtg agtacacgga aacagcttga ggcggatttg acggagctga agcggatggt
     3001 gctccgcctc ggcaagatgg ccgaggagtc catcagcaag gcgatctggg cgctgaagaa
     3061 tcaggatgcg gagcttgcca gaatggtgct ggcgaaggac gacgacatcg acgacctcgc
     3121 cgaagcgctg gacaacgcct gcatgcattt cgccgcgcgc ttccagcctt tgggcgagga
     3181 tctgcgggcg gtgacctcca tcatgcacat ggcggtggac ctggagcgca tcggcgatta
     3241 cggcggcaac atcgcgaagg tagcggcgga gcttgcggac aaggagctga tcaagcccct
     3301 gatcgacatt ccgcggatgc tcgacgtcct ttccgcgatg atgtcgaagg ctctggcggc
     3361 gttcgacacg aacaacgcgg agatggcgaa ggaggtcttc gccatggacg acatgatgga
     3421 tgatctggag aaacagatta tgagggaact gctgctcctg atgatggagc ggccgcagcg
     3481 catcgagcag gcgacgcagc tgctcaacgt ggcgcggacg ctggagaggg gcggcgatca
     3541 cgtgacgaac gtggccgaac gggtggtcta cattctcacc gggaagaccg tgaaggcttc
     3601 ggcgttccgg cgtccgcgcc ctgcggctct gaacggacga aaagaacacg aggagcggtg
     3661 agggacgtgc cggaagcgcg gattctcatt gtggaggacg aggacgcgat catggagatc
     3721 gtctctcagg cgctgaggcg gcacggctac gaaacgacct cggcctcgga cggcgacgcg
     3781 gcgctggaaa aaatcttctc tctccgtccc gacctggtga tcctcgacct gatgctgccg
     3841 aagatggatg gctgggaggt ctgccgccgg atgagagcgg agccggagac ggagcggatt
     3901 cccatcctga tgctcaccgc gcgccgcgac gagcgggacg tggtggaggg gctggagctg
     3961 ggggccgacg actacatgaa aaagccgttc tcgctcgcgg agctgacggc gcgcgtgaag
     4021 gcgctgctgc ggcgcaccgg ggagcggcgg gagagccgta ttgttgaaaa cggcgatctt
     4081 cgcatcgagc tcgacgacga gactgcgctg ctgcgcggga cgccgctcga tctgagcccg
     4141 acggagttcc ggctcctgga agcgctggcg cggaagatgg ggcggactgc ctcgcgcgag
     4201 gagctgctgt cgaagatatg gagcttctac ggcggcgaca cgcggaccgt ggacgtgcat
     4261 atctcccgcc tgcggaagaa gcttgacgac ggaaagtcgc cggcgttggc tatcaactcc
     4321 cttcgaggga gagggtatcg tctggtgtgg gagtgatgtc catgttcacg ttgaagaaaa
     4381 agattgtcgc ggtgatctcg ctggcggtga tgctcgcgtt cggtatcagc tggctgctgc
     4441 tgtacggggt ggtgcggaac ggcatcgtcc gacagagcac gctggagctt tcgcagcaga
     4501 cggcgcttct tgcggcgtcg ctgaaggacg gcggtatgga gcgatttacc gcgaatgtgg
     4561 aaaaatggga ggatattctc cgcggacggg tcacgctgat cggcgccaac ggcgtggtgc
     4621 ttgccgacac ggagcggagc ccggaggcga tggaaaacca cctgaccagg ccggaggtga
     4681 agggggcgct cgcttcgggc gaggggtccg cgctgcgcta cagcgcgacg acgaacatgt
     4741 actacctcta cttcgcccgc aaggtggagg ccgaggggcg cgtcgcggta ctcagggcgg
     4801 cgtacccgct ggagtttttg tcggaggcct tcgcggagac caggaacaag ttcatcttct
     4861 atcttttcgt cgccgtggtg ctgatccttg cgttcggcac gtggcttgcg cgattgttct
     4921 tccgtccgct cgaccggatc gtggagttcg cgggcaagat tgcgcagggg gaggcggtcc
     4981 gcttcccgat catggcggag ccggagctgc aacggctttc cagcgcgctc gacgggatgt
     5041 ccgcccggct tcgtgggg
//
LOCUS       DAOSWW010000063         5051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11123_length_5051_cov_4.035228, whole genome shotgun sequence.
ACCESSION   DAOSWW010000063 DAOSWW010000000
VERSION     DAOSWW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11123_length_5051_cov_4.035228"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /gene="ftsZ"
                     /locus_tag="PK442_02195"
     CDS             <1..918
                     /gene="ftsZ"
                     /locus_tag="PK442_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO86489.1"
                     /translation="VGMNSAKESIDKIREVVAGADMVFITAGMGGGTGTGASPVIAEV
                     AKEAGALVVAVVTFPFSFEMNRRRKQAAEGIATLKEKVDALLVVENDRLLELTDEKTK
                     HSEAYKLADEVLRQAVQGVTDLILKPSLINVDFADVRTVMQNAGSAIMGIGSGEGENR
                     AEIAAKSAIKSPLMSIPMKGAKGILFNITGGSDLGIHEIRKAAQIITETADEDAIVIW
                     GHTVAEDMAEKIQITVIATGFSSEKEKRTVTNFAAKQPAPKQKAPGVVLEEAEVLPIQ
                     DEDMFSISGVPKNPYDIPSIIRRNKKAGK"
     gene            1190..1831
                     /locus_tag="PK442_02200"
     CDS             1190..1831
                     /locus_tag="PK442_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOO86490.1"
                     /translation="MSIRESRRHKERRSVIPFGDIMLPVIGLVAVGLLIVGVKLFFLS
                     GDRYKTYTPLPIHQEEKTTPNPSVTPPVLPPGQPEKENAAEDAILAVPVSPGGGTGDR
                     VRVETPTKTPPVVKPKTPPRPPEVKRPAPPPAPSNSRWGVQIGSFTARQSAETVRQQA
                     ERAGFRVSILSGQVRGKTYFRVTVPAGNTRAEAVSLSQKLAKSGFPVFVVSIR"
     gene            1917..3149
                     /locus_tag="PK442_02205"
     CDS             1917..3149
                     /locus_tag="PK442_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOO86491.1"
                     /translation="MAGFVRELTDRMSAIEYAAASLSFPWQGNESSRDLDAAEGLTLA
                     RDILAPEDFPPFDRSTRDGYALISEDSYGASQSNPAFLTIRGEVPMAAVPSFSLSEGE
                     CALIHTGGVLPAGADAVIMLENVERTGNWLEIRKAVQKGENIVFRGEEFSAGAILLPK
                     GVQVDFKSSGVLAAAGISKAPVQHVKVGILSTGDEIVPCETRSLNPGQFRDANANILS
                     SLLRRHGYEPVYLGIVEDSKVSLEKALYRAMEECDVVLLSGGSSVSTRDHCSTILESL
                     EAPGLLVRGILMSPGKPTLIAGIKEKQKLVMGLPGHPFSCFLAAYTVFLPLAHAMNTG
                     AIVSPWRSVVLPVSESVFSHSGIEECLPCALRDGKAVPLPVKSSFAKALGQADGILRL
                     SSSRETVRPGEEVEVWLW"
     gene            3137..>5051
                     /locus_tag="PK442_02210"
     CDS             3137..>5051
                     /locus_tag="PK442_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO86492.1"
                     /translation="MALVKENMHITLKQAWTLLREAITQIAPELCSVQHCYERLLAAD
                     LLSQRNVPHYNASAMDGFALSSASTAHATPSSPVFLRRDEFARLNTGGVLPASFDAVA
                     MIEDVALSSDGEKLTVAKTLVAGENVRPVGEDVFRGQVIARVGERISPPLASLCATAG
                     IPSVSVQALPKTLFIPTGDEIIPLEEWLSMDEPPAGKVGESNSFLVQGYFSRWGFPVD
                     IAPCLPDNPEVLSEFLLKKAEEYDLLLVGAGSAKGEKDYTFSIFEKYGKPIFRWLLMK
                     PGRPASAALLRRAVAVNLPGFPMSNAVILWSIVYPILQLLQKGDFDESSAIQEALGAE
                     GSEEMSLLVSCSSSPGKEEWLRLKCVEIDGRKMAYPLPSGASALWSLAEIDAVSLLPL
                     ESAELPKGSAVSVWTTRRIPWKERILFQGSNDPALERLPSFVRKQGGDLLLRSVGSLA
                     GLAALSRRECHIAAAHLFHAETGEYNTPFIDELFHDPETVVRRRVFFREQGFITKKGN
                     PKKISGVRDLDREDIVLVNRQRGAGTRVLLDALLDEAGIHPSAVRGYNSLSPTHFESA
                     NRVALGFVDVALGIKSVADALDLDFIPVAEEPYELVYLKEFESLTGLKAVLNALDDKE
                     WRAGVLRMGGYRWA"
BASE COUNT         1231 a         1242 c         1358 g         1220 t
ORIGIN      
        1 gtgggtatga acagcgcgaa agaatccatt gataagattc gggaagttgt tgccggagcc
       61 gacatggtct ttattacagc ggggatgggc ggaggtacag gaacaggcgc ttctccggtc
      121 attgccgaag tcgccaagga agcgggagcg cttgttgtcg cagtcgtcac atttcccttt
      181 tccttcgaga tgaacaggcg ccgaaaacaa gcagccgaag ggatcgcaac gctcaaagag
      241 aaggtggatg cgctccttgt agtcgaaaac gacaggcttc ttgaactgac tgatgaaaaa
      301 accaagcatt cagaagccta taaacttgca gatgaagtac tccgtcaggc tgttcaggga
      361 gtgactgatc ttattttgaa accgagtctt atcaatgtcg attttgcaga cgtacgaact
      421 gtaatgcaaa atgccggttc agctattatg ggcattggct caggcgaagg tgaaaatcga
      481 gcggaaatcg cagcgaagag cgctatcaaa tcgcctctga tgtccattcc gatgaaaggc
      541 gccaagggta ttttattcaa cattaccgga ggctcggacc ttggtattca cgaaatccgc
      601 aaggcggctc agataattac ggagacagcg gatgaggacg ccatcgtcat ttgggggcat
      661 acagtcgcgg aggatatggc ggaaaaaata cagattacag ttatcgctac tggcttcagc
      721 tccgaaaaag agaagcgtac tgtcacgaat tttgcagcaa agcagcctgc gccaaaacaa
      781 aaggcgcccg gagtcgttct tgaagaagct gaagtactgc cgatacagga tgaggatatg
      841 ttttccatca gtggtgtgcc caagaaccct tacgatattc cttcgataat tcgaagaaat
      901 aaaaaagccg gcaagtaaat tctgatatcc cgaaaatcat ttcctcgagc gagttgaatt
      961 tgattgatcc ggaatcgtcc agcgcaggct gaaggaagca ggggtcttga aagggattcc
     1021 tgctttattt tttaggattc tgatctatta gataggcttg tgtgcactat tatagtaaat
     1081 acgttgcgct acaaactggg gttaatgcag aagacaaatt tttgctgatt taggaagcca
     1141 tttggggcac ttcttctcca agcgaggcag acggacaggg agggtaaaaa tgtccataag
     1201 agaaagcaga agacacaaag aaaggcgatc tgttatccct ttcggcgata ttatgcttcc
     1261 cgtcatcgga cttgtggcgg tcggcctcct gattgtgggt gtaaaactct tttttctttc
     1321 cggcgacagg tacaaaacgt atacaccgtt acctattcat caggaggaaa aaacaacgcc
     1381 caatccttcc gtcacacctc ctgttctccc gcccggacaa cccgagaagg aaaacgctgc
     1441 ggaggacgcc atcctcgcag ttcccgtttc acctggaggt ggcacaggag accgtgtcag
     1501 ggtagaaact cctacgaaaa ctcctccagt agtaaaacct aagacacccc cgcgcccacc
     1561 ggaagtcaaa cgacctgcgc ctccgccagc gccttccaac agtcgttggg gcgttcaaat
     1621 cggttcattt acagcacgcc agagcgccga gacggtacgt caacaggctg aaagagcggg
     1681 cttcagagtc tcgattttgt ccgggcaggt acgcgggaag acatatttca gggtgaccgt
     1741 tccggctgga aatactcgtg ccgaagcggt atctctctcg cagaagcttg caaaatctgg
     1801 ttttcctgtt tttgtggttt cgattcgata aagcgctttt ttgctttgta attatccact
     1861 tcatgtaggc ttcaacaaat tttggagagc acacggatcg ggaaggagat acgctattgg
     1921 ctggttttgt tcgggaactg actgacagaa tgtccgccat tgagtacgcc gccgcatcac
     1981 tctcatttcc ttggcaagga aatgagagtt caagagatct cgatgcagcg gagggtttga
     2041 ctcttgctcg agatattctt gcaccggaag atttccctcc ttttgacaga agcacccgcg
     2101 acgggtatgc gctcatcagt gaggacagct acggagcatc gcaatcaaat cccgcatttt
     2161 taacgatccg cggagaagtt ccaatggccg ccgttccttc gttttccttg agcgaagggg
     2221 aatgtgcgct gattcacact ggaggcgtac tgcctgcggg agcggatgcg gttattatgc
     2281 tagaaaacgt agagcgaaca ggaaattggc tggaaatacg aaaggctgtt cagaagggcg
     2341 agaatatcgt ctttcggggt gaagaattct cagcaggcgc catccttctc ccaaagggcg
     2401 tccaggttga cttcaagtct tccggcgttc ttgcagccgc aggtatttca aaggcgccgg
     2461 tgcaacacgt aaaggtaggt attctcagca cgggagacga aatagtcccc tgtgaaactc
     2521 gttctttaaa cccaggccag tttcgcgacg ccaacgcaaa cattctttcg tcgcttttac
     2581 ggcgtcatgg atatgaacct gtctatttgg gaatagtcga agactcaaag gtttctcttg
     2641 aaaaagcgtt ataccgagcc atggaggagt gcgacgttgt cctgctcagc ggggggtctt
     2701 cagtgagcac tagagaccat tgttcaacga ttcttgaatc cctcgaagca ccgggactgc
     2761 tggttcgagg cattttgatg tcgccgggga aacccactct catcgccgga ataaaagaaa
     2821 agcaaaagct cgtaatgggc ctgccgggac accctttttc atgttttctt gctgcgtata
     2881 ctgtgttcct tcctcttgca cacgctatga atactggcgc catcgtatcc ccttggagat
     2941 cggtagtact tcctgtttcc gagtctgtat tcagtcactc ggggattgag gaatgtttac
     3001 catgcgccct ccgcgacgga aaggctgttc ctcttccggt gaaatcttcg ttcgcaaagg
     3061 cgctcggaca agccgacggg attctccgtc tttcgtcttc gagagagact gtccgtcccg
     3121 gcgaggaggt agaagtatgg ctttggtaaa ggaaaatatg cacattacac tgaaacaggc
     3181 gtggactctt cttcgggagg caatcacaca gatcgccccc gagttatgtt ctgtacaaca
     3241 ttgttatgaa cgattacttg ccgcggatct tctctcccaa agaaacgttc cgcactacaa
     3301 tgcatccgcc atggacggat tcgcactctc atcagcctcc actgcccacg cgacgccatc
     3361 ttcgcctgta tttttacgcc gcgacgagtt tgctcggctc aatacgggcg gtgtgctgcc
     3421 tgcatccttc gatgcggttg ctatgattga ggacgtggcg ctctccagtg acggagaaaa
     3481 gctgaccgtc gccaaaacgc tcgttgccgg agaaaatgtt cgtcctgttg gtgaagatgt
     3541 ctttcgagga caggtaatag cgcgtgtagg ggagagaata tcgccgccgc tggcatcctt
     3601 gtgcgccacg gcgggaattc cttcagtcag cgtacaggct cttccgaaga ctctcttcat
     3661 ccctacagga gatgaaatca tccctctaga ggaatggctt tccatggacg agccgcctgc
     3721 tgggaaagta ggggagagca actcatttct cgttcagggc tacttttccc gatggggttt
     3781 ccctgtcgat atagctccat gccttcccga caatccagag gtactttcgg agtttctttt
     3841 gaaaaaagcc gaggagtacg acctgctgct cgttggggcc ggctcggcaa aaggagaaaa
     3901 ggactatact ttttccatct ttgaaaaata cgggaagcca atctttcgat ggcttctgat
     3961 gaaaccaggc agacctgcat ccgctgcgct gctacgacgt gcagtcgcgg tcaatctccc
     4021 gggttttcca atgtcgaacg ctgtcatcct ctggtcgatc gtctacccga ttttacagct
     4081 tcttcagaaa ggggattttg acgaatcttc cgcaatccaa gaagctctcg gagcggaagg
     4141 atccgaagag atgtctcttc tcgtctcctg ctcttcctcg ccgggaaaag aggaatggct
     4201 tcgtttaaaa tgcgtggaga tagacgggcg aaaaatggcg tatccgctgc cttcaggggc
     4261 aagcgcactc tggtccttgg cggagattga cgcagtgtcg ctgcttccgt tggaaagcgc
     4321 ggaattgccg aagggaagcg ccgtttcggt gtggacgacc cggcgtattc cctggaagga
     4381 acgcattctt ttccaagggt caaatgatcc tgccttggaa cgcctcccct catttgtccg
     4441 caaacagggc ggcgatctcc tgctcagatc ggtcggaagc cttgcgggac ttgccgctct
     4501 ttcgagaaga gaatgccata tcgcggcggc gcatctattc catgccgaga cgggagagta
     4561 caacactccg ttcatcgacg aactttttca tgatccggaa acggttgtcc ggaggagagt
     4621 tttctttcgg gaacagggtt ttattacgaa aaaaggcaac cccaagaaga tctccggggt
     4681 acgagatctt gaccgggagg atattgtgct cgtcaaccga cagcgaggcg cgggaacccg
     4741 tgtgctgctg gacgcgcttt tggacgaagc gggcattcat ccttccgcgg tcaggggata
     4801 caatagtctg agtccgacac attttgaatc agccaacaga gtagcgttag gctttgtcga
     4861 cgttgctctt ggtataaaat ctgtcgccga cgcgcttgac ttggatttca ttccagttgc
     4921 ggaagaaccc tatgaactcg tctacttgaa agaattcgaa agccttacag gactgaaagc
     4981 cgtgctcaac gcattggacg ataaggagtg gcgcgccggc gtactgcgta tgggaggata
     5041 ccgatgggca g
//
LOCUS       DAOSWW010000064         5045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11145_length_5045_cov_3.249900, whole genome shotgun sequence.
ACCESSION   DAOSWW010000064 DAOSWW010000000
VERSION     DAOSWW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11145_length_5045_cov_3.249900"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..1458)
                     /gene="iadA"
                     /locus_tag="PK442_02215"
     CDS             complement(289..1458)
                     /gene="iadA"
                     /locus_tag="PK442_02215"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HOO86493.1"
                     /translation="MLLIRNADLYAPERLGPGDILIAGERVLAVRPAGEIDSTAAASF
                     SDLRVIDAGGCIVAPGIIDRHVHFNGAGGEGGPAHRTPPLQLSSFIKAGVTSAVGMLG
                     TDGVCRSLNELLMKARGLEVEGISTWILTGSYSFPSPTLTGSVQSDLCLIDKVIGLKL
                     AVSDHRSSHPSVEEIRRAVSDARTGGILSGKVGCVCVHMGNEKEGLEPLLRAVEGTDV
                     PITQFAPTHISRTEALLEQSVEFGRRGGYLDVTADVGRKPLFGRGTSGVVDKLLSGGV
                     APENITISSDGNGSMATFDERGGVTGLIVAPLESVLESVVEMLRDGALPREQVFSFVT
                     ANPAAHLKLPRKGRLKPGFDGDLIMLDGAFRLRTVVAKGRVLMDEGEVVVKGTFE"
     gene            complement(1568..2971)
                     /gene="nhaC"
                     /locus_tag="PK442_02220"
     CDS             complement(1568..2971)
                     /gene="nhaC"
                     /locus_tag="PK442_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOO86494.1"
                     /translation="MEERKAREISLRGALMIFGAILAIIVVGRLIFTFDVALLLMFIG
                     MFTTFTYVYVYKFTWRDLFEGGVVPMIARASGAIMILLTVGPLIASWMLAGTIPYLIY
                     TGLQILSPNTFLMAAFVICALSSTMTGTSWGTAATFGVAFMGIAQGLGVPLPAAAGAV
                     VAGAYFGDKLSPVSDTTVLAAAVAEVDVVDHIRSMLWTTIPAFVISLGIYGYVGSQAS
                     GEIDLSSVNEIIAGISKTFKMTPLVLVPPLAVLVLAYRRFPTLPVLWIAVALAVPLAM
                     MQGYDLKEIMRAMAAGPKIVTEVASLDKLLSRGGLAFMSGSVVVVFFAYIFAGRLEYT
                     GTFRRICTSLKEGFIGNSKGRFVLSASLSGILTGLGTGNSYLSEIVPGTMYKDLADEM
                     NISRRVLSRTLEDSGTVVVPLIPWSAAGIYMSSVLGVSVFEYVPWAFLCWLGFLTAWF
                     YGFTGIAIWPSDKKADA"
     gene            3243..3932
                     /locus_tag="PK442_02225"
     CDS             3243..3932
                     /locus_tag="PK442_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO86495.1"
                     /translation="MENENINVNATEKAGREIVRLIASHRFKPGDRLLETSLAKILGM
                     SRTPVREALGRLASTGFLAKAEGTRGYQIPFLTPKDIEDVFFMRGILESKAAMLCAQT
                     MPESALVELRRINEREEETFRANEKGEYAELNESFHMLIAGSCANPYMEMYLRQLYWR
                     SNLYVLFFMSFYCLDSNEETERLSYLEHRRVIDALAGRDAKAAEAAMREHITSSYNHL
                     LYPKNALAGVE"
     gene            complement(3943..>5045)
                     /locus_tag="PK442_02230"
     CDS             complement(3943..>5045)
                     /locus_tag="PK442_02230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO86496.1"
                     /translation="AVTAALKGRRSVGLFELDGEPALLETRPVMLYGEIQVGTVGIGA
                     PLTKELLAHFSEGTGLGIEVEIEGFPPISMEECCGNTERKSVALFPSVSEESLKFRRI
                     EAVFTEDELGADLRYFQRNLVLTMLLLSVTLLSGLFLLLRRYLRPFSLLVEDVTLLSG
                     NQEDFFELRKRLAHNYLDTSHEVSVIAHSLSRLMERIQETITLLEWTSRTDPLTRISN
                     RLHLDSVLEQEMKASAKTGSPLSVLIFDLDHFKMVNDDFGHQAGDNVLRRTAEILKSL
                     VPVGFVGRWGGEEFLAVLPGAGEEEATEFGERIREAVENEPFPIGRRVTISAGVTEMI
                     PGDTPGSLVGRADKGLYEAKETGRNRVVLRKA"
BASE COUNT         1012 a         1518 c         1635 g          880 t
ORIGIN      
        1 tcggcgcccg gcttcagctc gacgagcgcc gcctgtgcgc aggacgcgaa cagcaacagg
       61 gcaaacaacg agagaaccac caagactctt ttcatgacac gcacctccct taatgcaccg
      121 gaaatccgcg gacaccggat ttcgtacgaa acggacgact tcgaccgcaa aaaacgccgt
      181 gtgcttggga aagtgtacaa gaagagtaat cacttgtcaa aaatgagaaa attaaagagg
      241 gagcgccaag cgatgcggcg ctccctctcc tctcttttca gagacgcgct actcgaaggt
      301 tcccttgacg acgacctcgc cctcgtccat cagcaccctt cccttggcga cgaccgtccg
      361 caggcggaag gcgccgtcga gcatgataag gtcgccgtcg aagccgggct tgaggcgccc
      421 cttgcggggg agcttgaggt gcgcggcggg gttcgcggtg acgaaggaga agacctgttc
      481 gcgcgggagc gcgccgtcgc gcagcatctc cacgaccgat tcgaggaccg attcaagcgg
      541 cgccacgatc aggccggtca ctccccctct ttcgtcgaag gtcgccatgg agccgttgcc
      601 gtccgagctg atggtgatgt tttcaggagc gacgcccccg gaaaggagct tgtcgaccac
      661 accggaggtt cccctgccga agagcggctt cctgccgacg tcggcggtga cgtcgagata
      721 cccgccccga cgcccgaact cgacggactg ctccagcagc gcctcggtgc gcgagatgtg
      781 cgtgggcgcg aactgggtaa tcggcacgtc ggtcccctcg acggcccgaa gcagcggttc
      841 gagtccttcc ttctcgttgc ccatgtggac gcagacgcat ccgactttgc cggagagtat
      901 acccccggta cgggcgtccg aaacggcgcg gcgaatctcc tcgacggaag ggtgcgagct
      961 ccggtggtcg gagacggcga gcttcagccc gatgaccttg tcgatcagac agaggtcgct
     1021 ctggacgctt ccggtaagcg ttggcgacgg aaaagagtag ctccctgtga gaatccacgt
     1081 ggagatccct tccacttcca gtcccctcgc cttcatcagc agttcgttca gagagcggca
     1141 aaccccatcg gtccccagca tgccgaccgc ggaggtgacg cccgccttga tgaacgacga
     1201 aagctggagc ggcggcgtcc tgtgggcggg tcctccctcg ccgcccgcgc cgttgaagtg
     1261 aacgtgccgg tcgatgatgc cgggcgcgac gatgcagccc ccggcgtcga tcacccggag
     1321 atcgctgaac gaggcggccg ccgtggaatc gatctccccc gccgggcgga cggcgagcac
     1381 cctctccccc gcgatcagga tgtcgccggg accgaggcgc tcgggagcgt agagatcggc
     1441 gttgcgtatc aagagcattg gtgtgtctcc tttcccgcgt ccggcccgtg ggcggcgcgt
     1501 tgcgatgcgg agaggaggga ggttccctcg cttggcgccg aaggaaacct ccctcccggc
     1561 agacgcgcta cgcgtccgcc ttcttgtcgg agggccagat cgcgattccg gtgaagccgt
     1621 agaaccaggc ggtcaggaag ccgagccagc agaggaatgc ccagggaaca tactcgaaga
     1681 cggaaacgcc gagcaccgag gacatgtaga ttccagcggc cgaccagggg atcagcggaa
     1741 cgaccacggt gccggagtcc tcgagcgtac gggagagcac gcggcgggag atgttcatct
     1801 cgtcggcgag gtctttgtac atcgtgcccg ggacgatctc gctgaggtag gagttgccgg
     1861 tgcccagtcc ggtgaggatg ccggagagcg aggcggagag gacgaagcgc cccttgctgt
     1921 tcccgatgaa gccctccttc agggaggtgc agatgcggcg gaaggtaccc gtgtactcaa
     1981 gccgtcccgc gaagatgtac gcgaagaaga ccacgaccac cgaacctgac atgaaggcga
     2041 gtccgcccct gctgaggagc ttgtcgaggc tggcgacctc ggtgacgatc ttcgggccgg
     2101 cggccatcgc gcgcatgatc tccttcaggt cgtacccctg catcatggcc agaggcacgg
     2161 cgagcgcgac ggcgatccag agcaccggga gggtcgggaa gcgccggtag gcgaggacga
     2221 ggacggcaag cggcggaacg aggacgagcg gcgtcatctt gaaggtcttc gagatgccgg
     2281 cgatgatctc gttcacgctg ctgagatcga tctcgccgga ggcctgcgat ccgacgtagc
     2341 cgtaaatgcc gaggctgatg acgaacgccg gaatggtggt ccagagcatg gagcggatat
     2401 ggtccaccac gtcgacctca gcgaccgccg cggcgagaac cgtggtgtcg gagacggggc
     2461 tgagcttatc gccgaagtag gctcccgcga ccaccgcgcc ggcggccgcc ggaaggggta
     2521 cgccgagccc ctgcgcgatc cccatgaagg cgacgccgaa ggttgcggca gtcccccacg
     2581 aggttcccgt catcgtggac gagagggcgc agatgacgaa ggccgccatc aggaacgtgt
     2641 tcggactgag gatctgaagt cccgtgtaga tcaggtaggg gatggtgccc gcgagcatcc
     2701 acgacgcgat gagcgggccg acggtgagca ggatcatgat ggcgcccgac gcgcgggcga
     2761 tcatgggaac gacgcccccc tcgaagaggt cgcgccaggt gaacttatag acatacacgt
     2821 acgtgaaggt ggtgaacatg ccgatgaaca tcaggagcag ggcgacgtcg aatgtgaaga
     2881 tcagtcttcc gacgacgatg atcgccagga tggcgccaaa aatcataagt gcgccccgga
     2941 gcgatatttc acgtgctttc cgttcttcca tgtggacgat aacctccttg tgccgagtgg
     3001 ctcataagaa ataaatgcgg acgaacttga cgtagggaga agctgttttc actcttctcg
     3061 gcgtttcact ccccctttct ggattctttt atggatacgt tattgtataa tcattatctc
     3121 tcgcgagcgt tttgtcaaga gtttttttaa aaaaggcgtc cggaagaatg catctttatc
     3181 ggatatctgc tacacttttg gagtcccttc gtaagggaaa agagaaaatt gcgaggtgct
     3241 tcatggaaaa cgagaacatc aacgtcaacg cgaccgagaa ggccgggcgg gagatcgtcc
     3301 ggctcatcgc gtcgcatcgt ttcaaaccgg gagaccgtct gctggagacg tcgctcgcca
     3361 agattctggg aatgagcaga acgcccgtca gggaggcgct gggaaggctg gcgtccacag
     3421 gttttctggc gaaggcggag gggacgaggg ggtaccagat ccccttcctg acgccgaagg
     3481 atatagagga tgtcttcttc atgcggggaa tccttgagag caaggcggcg atgctctgcg
     3541 cgcagacgat gccggagagc gcgctggtcg agctgcgccg gatcaacgag cgcgaggaag
     3601 aaacgttcag ggcgaacgag aagggggagt acgcggagct gaacgagtcg ttccacatgc
     3661 tcatcgccgg ctcctgcgcc aacccgtaca tggagatgta cctcaggcag ctctactggc
     3721 ggtcgaacct ctacgtcctc ttcttcatga gcttctactg cctggattcg aacgaagaga
     3781 cggagcgcct gagctatctc gagcaccggc gcgtgatcga cgcgctcgcc gggcgcgacg
     3841 cgaaggccgc ggaggccgcg atgcgggagc atatcacaag cagctacaac cacctgctct
     3901 atccgaagaa cgcgctggcc ggggtcgaat agacggcggg cctcacgcct tccggagcac
     3961 cactctgttg cgcccggtct ccttggcttc gtagagtccc ttgtcggcgc gaccgacaag
     4021 ggatcccggg gtgtcgccgg ggatcatctc cgtaactccc gcgctgatgg tcactctgcg
     4081 gccgatgggg aacggctcgt tctccacagc ctcccggatg cgctcgccga attcggtcgc
     4141 ctcctcttcg cccgcgccgg gaaggaccgc gagaaactcc tcgccccccc accggccgac
     4201 gaaccccacc gggacgaggc tcttcaaaat ctccgcggta cggcggagca cgttgtcccc
     4261 cgcctggtgg ccgaaatcgt cgttcaccat cttgaaatgg tcgaggtcga agatgagcac
     4321 cgagagcggc gagcccgtct tcgcggatgc cttcatctcc tgttcaagca cgctgtcgag
     4381 gtggagccgg ttgctgatcc tggtgagcgg gtcggtgcgg gaggtccatt cgagcagcgt
     4441 gatcgtctcc tggatgcgct ccatcagtct ggagagggag tgcgcgatca cggagacctc
     4501 gtggctcgta tcaaggtagt tgtgcgcgag ccttttgcgc agttcgaaga agtcctcctg
     4561 atttccggag agcagcgtca cgtcctccac gagcagcgaa aaggggcgga ggtagcggcg
     4621 aagcagcagg aagaggccgg agagcagcgt tacggagagg aggagcatcg tcagcacgag
     4681 atttctttga aaataccgca ggtccgcgcc gagctcgtcc tctgtgaaca ccgcttcgat
     4741 ccgccggaac ttcaggctct cctccgaaac ggaaggaaac agcgcgacgg acttcctttc
     4801 ggtatttccg cagcattcct ccatggaaat gggcgggaac ccctctatct cgacttctat
     4861 ccccagtccg gtaccctcgg aaaagtgcgc gaggagctcc ttcgtcagag gggcgccgat
     4921 gccgaccgtt cccacctgaa tctctccgta gagcatgacc ggacgcgttt cgagcagcgc
     4981 cggttcaccg tccagttcga acagtccgac ggagcgtcgc cctttcagag ccgcggtgac
     5041 cgctg
//
LOCUS       DAOSWW010000065         5041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11159_length_5041_cov_4.499799, whole genome shotgun sequence.
ACCESSION   DAOSWW010000065 DAOSWW010000000
VERSION     DAOSWW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11159_length_5041_cov_4.499799"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PK442_02235"
     CDS             complement(<1..763)
                     /locus_tag="PK442_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO86497.1"
                     /translation="MKRNWMIALVVLVVVAGYFFMQQQAPQPPKEEPVKESPTPTPEA
                     PKAEEPKAEEPKAEEPKAEEPKAAEETVKIGYLAALTGDWAAYGQTEEKTARLAVDEI
                     NAKGGVLGKKLELVVYDFRTRAEDAVNAVRRMIEEDKVVAIVGANGSGINIATAPIVN
                     RLGVSQIGTVSTNPLVTVDEKGEVRPYSFRICFTDPYQGKLIAFFAAKELKRMKAAIL
                     YDVGNDYSQGLREFFMKSYAEYQGEVVADLGFRGGT"
     gene            complement(1049..2278)
                     /gene="rocD"
                     /locus_tag="PK442_02240"
     CDS             complement(1049..2278)
                     /gene="rocD"
                     /locus_tag="PK442_02240"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOO86498.1"
                     /translation="MMSRNRSEELMEMEHEYGAHNYHPLEVVISKAEGIWVEDPEGRR
                     YMDMLSAYSAVNQGHRHPKIIKALKDQADILTLTSRAFFNDRWPVFAKKLADFTGKEM
                     ILPMNTGAEAVETALKTMRKWGYMVKGVEKDKAEIIVFENNFHGRTITIISFSIDPDA
                     TDYYGPFTPGFKVVPYDDLEAVEKAVTKNTVGIMVEPIQGEAGVVVPSDGFLKGLETI
                     ARKHNVLLAIDEIQTGFCRTGKNFAFQHEGIDPDIIIMGKALGGGVFPVSAIAANKDV
                     LGVFKPGTHGSTFGGNPLACAVASAAIDVLVDEKMVERAAEMGKYFMDGIKAIQSPKI
                     KLVRGKGLLIGVVLNESAGKARLYTTALKEKGLLCKETHDWIIRFAPPLVITKQEIDQ
                     ALKTIQEVFASTPIPCV"
     gene            complement(2563..4200)
                     /locus_tag="PK442_02245"
     CDS             complement(2563..4200)
                     /locus_tag="PK442_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO86499.1"
                     /translation="MLSLAENDLYSLLLDLVAIPSVSFSEEENRAADFIFSTLSDLEY
                     FRRNPSFLRFLPAEGDPLGRKAVAALVKANPRTKKTVIITGHFDVVDAEACGHLKNLA
                     FSPEEYTRRVDELEIPEEARDDLASGEYLFGRGVSDMKSGIALEMCLIGALSRLESFP
                     ANVMFLAVPDEENTSAGMRGAVPWLVRLRNEWELEYTACLNSEPSVGGRGIPAGGVYV
                     GTIGKIMPFYLCVGKEAHVGDYYEGVSASLLTSYLSLILEGSPETAESLDGVHFPPQA
                     CLRFKDLVKNYSVTLPERSVAYYNCLTVSRTPARILEEMKTVAGRALEMTLTHLSKAR
                     QTLRERGASLPEKKAFPPRVLSYGDLLEKVRSRTERFDDVLESFLKTLPDSMDERDLC
                     IETASFLLDLSGEKGPLILVGFLPPFYPPRLNRSESAGERALLRAVERLKEAGKPYGL
                     SISRIDVFQGIMDLSYLGFQGAPSELDALAENMPLWGRGYRFPLNELKKLDVPIANFG
                     PIGKDDHKNAERIHLPYYLHTLPPLFFKFVEFLAEES"
     gene            complement(4207..>5041)
                     /locus_tag="PK442_02250"
     CDS             complement(4207..>5041)
                     /locus_tag="PK442_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOO86500.1"
                     /translation="LVIAVLLFLAAFFLSGRLTDFLSRTLLSRFGLDSTAMMASRKIL
                     FFILMGALILAALDLVGIPLTAFAFLGGALAIGIGFGAQNIFNNLISGFILMFSEPMR
                     VDDTIEIDGLFATVQEIGTRATRVKTFDNIDVLMPNSYFLNNKIVNWTLTDQKIRLRI
                     NVGVVYGSDARKVEELLLLAAHDHTRVLRNPEPFVVFREFGPSALEFTLYFWIDMTNA
                     STLKVSSDLRYRLIALFEKNGIKIAFPQMDVHLDTLSPVDICLNRPRTADTEQTHTQE
                     E"
BASE COUNT         1152 a         1390 c         1304 g         1195 t
ORIGIN      
        1 ccgtgccgcc gcggaatccg aggtcggcga caacctcgcc ctggtactcg gcgtagctct
       61 tcatgaagaa ctcacgaagg ccctgagaat agtcgttccc cacgtcatac agaatagcgg
      121 ccttcattct cttaagttct ttcgccgcaa agaaagcgat cagctttccc tggtaggggt
      181 cggtgaagca tatacggaat gagtacggac gaacctcgcc cttctcatcg acggtgacga
      241 gaggattcgt tgaaacggtg ccgatctggg atacgccgag acggttcaca atgggcgcgg
      301 tagcgatgtt gatgccgctg ccgttggctc cgacgatagc cacaaccttg tcttcttcga
      361 tcatacgccg gacggcattc acagcatctt cggcgcgcgt acggaagtca tagacaacga
      421 gctccaactt ctttccaagg acgccgcctt ttgcgttgat ttcatcaact gcaagacggg
      481 ccgttttctc ctcggtctga ccgtaggccg cccagtcgcc tgtaagagcg gcaagatagc
      541 cgatctttac agtctcttct gctgccttgg gttcttcggc tttcggctct tctgctttcg
      601 gctcctcagc ctttggctct tcagccttcg gcgcctcggg cgtcggcgta ggagattcct
      661 tcaccggctc ctccttcggg ggctgcggcg cctgctgttg cataaagaaa tacccggcga
      721 cgacaacaag cactacgaga gcgatcatcc aattccgttt caaaaccaac cacctcctga
      781 gatttttgtg ttccaccgta cggtttcact gcttttcaga agtcatttta ctacgtcgcg
      841 ctctatctct caaatatttt cggctctttt ggggctcttc aagaaagaag ttttccgtag
      901 agacaatttc tgttttggag cttcgttgac gtccgtcatt ttttcataca tttaaggaac
      961 acgcgattca ctgcaagcga gttgcatccc ataaagtttg cggcagcctc ttgaggaagc
     1021 tgccgcaaac gcatcggggc ggattctttt acacgcaggg aatcggcgtc gaagcaaaca
     1081 cttcctgaat agtcttcagc gcctggtcga tctcttgctt tgtgatgaca agaggcggag
     1141 caaaacggat gatccagtcg tgcgtctcct tacagaggag gcccttctct ttcagcgccg
     1201 tcgtatagag acgggccttc cctgccgact cgttgaggac aacgccgatg agcaggcctt
     1261 tacctcgcac aagcttgatc ttcggcgatt gaatcgcctt gataccgtcc atgaagtatt
     1321 ttcccatctc ggccgcgcgc tctaccatct tttcgtctac gagcacgtcg atagcagcgc
     1381 tggcaacggc acaagccaac ggattgcccc cgaaggtcga accgtgggtt cccggcttga
     1441 acacacccag tacatccttg ttcgccgcta tggcggaaac cggaaagact ccgccgccaa
     1501 gcgcctttcc catgatgatg atgtcgggat cgattccctc gtgttggaac gcaaaattct
     1561 tccctgtgcg gcagaaaccg gtctgaatct cgtcgatggc cagaagcaca ttgtgcttcc
     1621 tggcgatcgt ctccagcccc ttcaggaaac cgtcgctcgg aacgacgacg ccggcctcac
     1681 cctggatggg ctcgaccata atgccgactg tatttttcgt caccgctttt tcgaccgctt
     1741 cgaggtcgtc gtaaggaact actttgaatc cgggggtgaa cgggccgtaa taatccgtgg
     1801 cgtcgggatc tatcgagaag gaaataatgg taatggttcg tccgtgaaag ttgttttcaa
     1861 agacgatgat ctccgccttg tccttctcaa ctcccttgac catgtacccc cattttctca
     1921 tagtcttcaa agccgtttcc accgcctcgg ctccggtatt catggggagt atcatctctt
     1981 ttcccgtgaa atcggcaagt ttcttcgcga agaccggcca acggtcgttg aagaaggcgc
     2041 gggaggtcaa ggtgaggata tcagcctgat ctttcaaagc tttgatgatc tttggatgtc
     2101 tgtgcccctg gttgactgcg gagtatgcgg ataacatatc catgtaccgc cgcccctccg
     2161 ggtcctcgac ccaaatacct tcggctttcg agatcacgac ttcaagcgga tgatagttgt
     2221 gggcgccgta ttcgtgttcc atctccataa gctcttccga tctgtttcga gacatcatgc
     2281 cacctctttc tgaaggtata gtaaatttcg tttcactatt tctaaggcgt gcgaaacgat
     2341 gtttacaaaa agtagtgcat ccactattgc tattccagaa ttgattcttt cattgaatca
     2401 ttatccacca taatcctctt tttctcaact ccggaaaaac aaaaatataa accctcagaa
     2461 gaggagaagg tttcctgtat atactttgaa atgcaaaagc gggttttcgt ctccgaaaac
     2521 ccgcttttat aatcagtatt gatcagtagc tccaccctct ttttaagatt cttcagcgag
     2581 aaactccacg aatttgaaaa aaagcggggg aagcgtgtga aggtagtacg gcaggtgaat
     2641 acgctcggcg ttcttgtggt catcctttcc gatcgggccg aagtttgcta tcggaacgtc
     2701 gagttttttc agttcgttaa gagggaaacg gtaccctctc ccccagagag gcatattctc
     2761 cgccagggcg tcaagctccg acggcgctcc ttgaaacccg aggtagctca agtccataat
     2821 tccttgaaaa acatcgattc tacttatgga aaggccatac ggctttccag cctccttgag
     2881 acgttccacc gcccgcagca gagcgcgttc tccggcgctc tcgctcctgt tgaggcgcgg
     2941 agggtagaac ggcggtaaaa atccgacgag aatgagcggc cccttctccc cggaaagatc
     3001 gagaagaaag gacgccgttt cgatacagag atcgcgctcg tccatggagt caggaagcgt
     3061 cttcagaaaa gattcaagca cgtcgtcgaa tcgctccgtc cggctacgga ctttctccag
     3121 aagatctccg tacgaaagga cgcgcggagg gaacgccttc ttctcgggga gcgaagcccc
     3181 tcgttcgcgc agcgtctgcc tggctttcga caggtgggta agcgtcatct caagagcccg
     3241 tccggcgacc gttttcattt cctcaagaat ccgggcgggc gtccgggaaa cggtgaggca
     3301 gttgtaatac gccacggagc gttcggggag cgtcacagaa tagtttttta ccagatcctt
     3361 gaagcgaagg caggcctgcg gcggaaaatg aaccccgtcc agggattccg cagtctccgg
     3421 gcttccctca aggatgagcg aaagatacga cgttaaaagc gacgcgctga ccccctcata
     3481 gtagtcgccg acgtgcgcct ccttccccac gcagaggtag aatggcatta tctttcctat
     3541 tgtaccgacg tagacgcctc cggcaggaat gccccgcccg ccgaccgacg gttcgctgtt
     3601 gaggcacgct gtgtattcga gctcccattc attacgaaga cgtacaagcc aggggacagc
     3661 cccccgcatt cctgccgagg tgttctcctc atcggggaca gcgagaaaca taacgtttgc
     3721 agggaaactc tccaaacgtg agagcgctcc tataaggcac atctcaagcg ctattcctga
     3781 tttcatgtcg gaaactccgc gcccgaagag atactctccg ctcgccagat cgtcccgagc
     3841 ctcctccggt atctccagtt cgtccacacg gcgggtatac tcttcggggg agaatgccaa
     3901 atttttgagg tgaccgcacg cttccgcatc caccacgtcg aaatgtcccg taataatgac
     3961 ggtctttttc gtccgggggt tcgccttcac gagcgcagcg acggccttgc gtccgagcgg
     4021 gtctccctct gctgggagga aacgcagaaa ggaggggttt cgccgaaagt actcaagatc
     4081 cgagagcgta gaaaaaataa aatccgcagc ccggttctcc tcctcggaga aggagacgct
     4141 gggtatggcg acaagatcaa gaagcagcga gtacaggtcg ttttctgcaa gcgagagcat
     4201 gaaatgctat tcctcctgtg tatgtgtttg ttcagtatcg gcagtacgcg gacggttcag
     4261 acaaatatcg acaggcgaga gagtatccag atgcacatcc atctgcggaa aggcgatctt
     4321 gatcccgttt ttttcaaaga gggcgatgag gcgatagcgc agatcgctcg acactttgag
     4381 cgtcgaagcg ttcgtcatat cgatccagaa ataaagtgtg aattcgagcg cgctcgggcc
     4441 gaactcccgg aagacaacga agggctccgg attacgcagt acacgcgtat gatcgtgggc
     4501 tgcaagcaga agaagctcct ccacttttcg ggcgtcggaa ccgtacacaa cgcctacgtt
     4561 gattctcagg cgaatcttct ggtcagtgag ggtccagttg acaattttat tattaagaaa
     4621 atagctattg ggcatgagca catcgatgtt gtcgaacgtt tttactcgcg ttgcgcgggt
     4681 gcctatctcc tgcaccgtcg cgaaaagacc gtctatctcg atcgtatcgt ccactcgcat
     4741 cggttcgctg aacataagaa taaaaccact gatcaggttg ttgaaaatat tctgggctcc
     4801 gaaaccgatg ccgatggcaa gggcgcctcc gaggaacgcg aacgccgtaa gcggtatgcc
     4861 gacaagatcg agcgcggcca gaataagggc gcccatcagg atgaaaaaga gaattttcct
     4921 gctggccatc atcgcggttg aatccagacc gaaacggcta agcaacgtcc tgctgaggaa
     4981 atcggtcaga cgtccgctca gaaagaacgc agcgaggaag agtaataccg cgattaccag
     5041 t
//
LOCUS       DAOSWW010000066         5034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11178_length_5034_cov_4.408516, whole genome shotgun sequence.
ACCESSION   DAOSWW010000066 DAOSWW010000000
VERSION     DAOSWW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11178_length_5034_cov_4.408516"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK442_02255"
     CDS             complement(<1..424)
                     /locus_tag="PK442_02255"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO86501.1"
                     /translation="MRLENKTAIVTGGARGIGQAMCELFASEGARVLAADMSELSYEN
                     PSVEGYRLDVSDPEACRACFEYAVGRYGKIDVLVNNAGITRDALTRKMTDEMWHKVIG
                     VNLSGVFNLGRLVGPHMQEHGGGSIVCISSVVGEFGNIG"
     gene            complement(442..1635)
                     /locus_tag="PK442_02260"
     CDS             complement(442..1635)
                     /locus_tag="PK442_02260"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOO86502.1"
                     /translation="MSERIVLAGAVRTPIGKFGGTLAEVPVEKLGALVIKEALRRAGV
                     APGQVDEVLMGCILQACRGQNVARQAAIGAGLPVEVPATTLNNVCGSGLKAVNLAADM
                     IRSGSAEIVVAGGMENMSAAPYALEKARSGYRMGDGRCVDTMVLDALTDAFNNYHMGV
                     TAENVAEKYGITREMQDEFAAESQRKCEAAWNAGRFDEEIVPVPVTVKKQTVLFDRDE
                     HPRAGVSSGELAALKPAFKKEGGTVTAGNASGINDGAAAVVVMSEQKAKELGVVPMAV
                     FEGGASAGVDPCVMGIGPAFSTRKLLERTGRGIAEFDLIEANEAFAAQALAVGKLLEW
                     DWNRVNVNGGAIALGHPVGASGCRILVTLLHEMRRRGVRNGLATLCVGGGMGVSTAVG
                     LYDGR"
     gene            complement(1649..2065)
                     /locus_tag="PK442_02265"
     CDS             complement(1649..2065)
                     /locus_tag="PK442_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOO86503.1"
                     /translation="MKGKTIKEIHAGDTASFQKTLSESDVYLFAGVSGDQNPAHINET
                     YASGTRFKTRIAHGMLVASLISAALGMELPGPGTIYLGQTIKFIAPAHIGDTVEARVT
                     VTEIDERRNIVTLETVCTNQDGKVLLEGVATVMPPK"
     gene            complement(2121..3158)
                     /locus_tag="PK442_02270"
     CDS             complement(2121..3158)
                     /locus_tag="PK442_02270"
                     /inference="COORDINATES: protein motif:HMM:NF005308.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOO86504.1"
                     /translation="MGVTLLKSADLVGIRSIGSYVPSGIRGSAYIAAASGIPEKVIRE
                     KFGINLVHKAPPEESVSAMGAKAALRALEGIDPASLDLVVYCGSEYKDYYLYNCAAKI
                     QHEIGAVNANAFEIHSLCSAGVYSLKVLKGMMLADDDLKRVLLVSSSKEGDLINYADG
                     ESRFMFNFGDGAAAAVLEKGLARNVVLETAMITDGSFADDIGVMGIGCKNFAGFSDMP
                     YGDRFLKVADIARMKERLDPVTLDNFVRVIERAAEKSGYAKDAIGFIAPIFMKRSILE
                     TMLASFGLSEENSFVLEDYGHCQSADAFIALQNGESLGRLKEGDVAVLVAAGTGYTWA
                     ATAVKWGAWSA"
     gene            complement(3159..4721)
                     /locus_tag="PK442_02275"
     CDS             complement(3159..4721)
                     /locus_tag="PK442_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO86505.1"
                     /translation="MPNSWIGNYSFFRARIDSSREAVYDLDTETRYTYGDLERRANLL
                     ARYLKDQAGVRKGDRVAFIARNCIEMIDAYYATGKLGAIFVPYNARLSVSELVQLVNN
                     ETPKVLFYEEVFADKIPHLKSGCSVEKYLVLSPGKNPENDATYAEATGHGNDAFVGCD
                     ELDIEDIHMIIHTGGTTGLPKGGLISHRCELFNCLNEICTWNLHHEDSTLLLLPLFHT
                     GGWNLLTLPLLHVGGRLVINRQFDPKKTLEIVEKERITTLFGAATIFRMMVELPEFAS
                     ADFSSVKWIMAGAAPTPVNIMEKFWNKGVKFVLGYGMTEAGPNNISSCAHLISDDVMK
                     AKYASVGKPMYLSMVKLADDDGNEISEPNTPGELLWGGPQTFSGYWNNPDETAKTLKD
                     GWVHSGDMAVRDEDGYYYIVGRKKNMFISGGENVFPPEIERAMYDIAEIHEVCVFGVP
                     DEKWGEVGKAVVSLKPGKRVSKEEIVAALGSKLARYKIPKYIAFVDEVPKNNVGKIVV
                     SKVVELYGRATD"
     gene            complement(4734..>5034)
                     /locus_tag="PK442_02280"
     CDS             complement(4734..>5034)
                     /locus_tag="PK442_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86506.1"
                     /translation="GRWAKNEMKKEGESYTWGTTALSGKVVVPVSTARRAGHNFAFAQ
                     MLLWAAANDCIIVGNTYWNVGVAGKGGAKNAEEDEEGVGIMTNIADRVVALLKRL"
BASE COUNT          779 a         1779 c         1490 g          986 t
ORIGIN      
        1 gtccgatgtt gccgaactcc cccacgacgg aggagatgca gacgatggag ccgcccccat
       61 gctcctgcat gtgcggcccg acgagccgtc cgaggttgaa gacgccgctc aggttgacgc
      121 cgatgacctt gtgccacatc tcgtcggtca tcttccgcgt cagcgcgtcg cgggtaatcc
      181 ccgcgttgtt cacgaggacg tcgattttgc cgtaccgccc gaccgcgtac tcgaagcacg
      241 ccctgcacgc ttccgggtcg gagacgtcga gcctgtaccc ctcgaccgac ggattctcat
      301 acgacagctc cgacatgtcc gccgcgagga cgcgcgcgcc ctccgaggcg aagagttcgc
      361 acatcgcctg cccgatgccg cgcgccccgc cggtgacgat ggcggtttta ttctcaagtc
      421 tcattcgcac atctccttcc atcaccggcc gtcgtaaagt ccgacggccg tcgagacgcc
      481 catcccgccg ccgacgcaga gcgtggcgag cccgtttcgg acgcctcttc gccgcatctc
      541 gtggagcagc gtcacaagga tccggcagcc ggaagcgccc acggggtgcc cgagggcgat
      601 ggcccctccg ttcacgttca cgcggttcca gtcccactcc agcagctttc cgacggcgag
      661 cgcctgggcc gcgaacgcct cgttcgcctc gatcagatcg aactccgcga tgccccgccc
      721 cgtccgctcc agcagctttc gcgtgctgaa cgcggggcct atccccatga cgcaggggtc
      781 gacccccgcc gacgcgccgc cctcgaagac tgccatcggc acgacgccca gctccttcgc
      841 cttttgctcg ctcatgacga cgaccgcagc cgccccgtcg ttgatgccgg aggcgttccc
      901 cgcggtcacc gtccccccct cctttttgaa ggcgggtttc agcgccgcca gctccccgga
      961 ggagacgccc gcgcgagggt gttcgtcgcg atcgaagaga acggtctgct tcttgaccgt
     1021 caccggaacg gggacgatct cctcgtcgaa ccgcccggca ttccacgccg cctcgcactt
     1081 ccgctggctt tcggcggcga actcgtcctg catctcgcgg gtgattccgt acttttcggc
     1141 cacattctcg gccgtcacgc ccatgtggta gttattgaag gcgtccgtca gcgcgtcgag
     1201 caccatggtg tccacgcagc ggccgtcgcc catgcggtag ccggaccgcg ccttctccag
     1261 cgcgtagggg gcggcggaca tgttctccat gcctcccgcc acaacgatct cggcgctccc
     1321 ggagcggatc atgtccgcag ccaggttcac cgccttgagg ccggagccgc agacgttgtt
     1381 cagcgtcgtc gcgggaacct ccacggggag ccccgcgccg atcgccgcct gccgcgccac
     1441 gttctgcccg cggcaggcct gaaggatgca ccccatcagg acttcgtcca cctgaccggg
     1501 cgcaacgccc gcgcgccgaa gcgcttcttt gatcaccagc gcgccgagct tttcgacggg
     1561 gacctcggcg agcgtcccgc cgaacttccc tatcggggtg cgcaccgcgc ccgccagcac
     1621 tatccgttcg ctcatcgcat tccctccctc acttcggcgg catcacggtc gcgacgcctt
     1681 cgagcagcac cttcccgtcc tgattcgtgc agacggtctc cagcgtgacg atgttcctcc
     1741 gctcgtcgat ctccgtgacg gtcaccctcg cctccaccgt gtcgccgata tgggcggggg
     1801 ctatgaattt gatcgtctgc ccgaggtaga tggtccccgg cccgggcagc tccatcccga
     1861 gagcggcgga gatcagcgag gcgacgagca tgccgtgggc gatgcgggtc ttgaatcgcg
     1921 tgccggaggc gtacgtttcg ttgatgtggg ccggattctg atctccgctc accccggcga
     1981 agaggtacac gtccgactcg ctcagcgtct tctggaacga agccgtgtcg cctgcatgga
     2041 tctccttgat tgtctttccc ttcatagcgt cctccttcgg cgcccccgga cggggggctt
     2101 ctcctccgtg cctcctccgg ctacgcgctc cacgcgcccc acttcaccgc cgtagcggcc
     2161 catgtgtacc ccgtgcccgc cgcgacgagg acggcgacgt ccccttcctt cagcctcccc
     2221 agcgactctc cgttctggag ggcgatgaac gcgtcggccg actgacagtg gccgtagtcc
     2281 tccagcacga acgagttctc ctcgctcagc ccgaagctgg cgagcatggt ctcgaggatg
     2341 gagcgcttca tgaagatggg ggcgatgaag ccgatggcgt ccttggcgta cccgctcttc
     2401 tccgccgccc gttcgatcac ccggacgaag ttgtccagcg tgaccggatc gagccgctcc
     2461 ttcatccggg cgatgtccgc gaccttcaaa aagcggtcgc cgtacggcat gtcgctgaag
     2521 ccggcgaagt tcttgcaccc gatccccata acgccgatgt cgtccgcgaa gcttccgtcg
     2581 gtaatcatgg cggtctccag cacgacgttc cgcgccaacc ccttttcgag caccgccgcg
     2641 gccgcgccgt cgccgaagtt gaacatgaag cgcgattcgc cgtcggcgta gttgatcagg
     2701 tccccctcct tggaggagga gaccagcagg acgcgcttca gatcgtcgtc cgcaagcatc
     2761 atgcccttca gtaccttgag cgaatagacc cccgccgaac agaggctgtg aatttcgaag
     2821 gcgttcgcgt tgaccgcccc gatctcgtgc tggatctttg ccgcgcagtt gtagaggtag
     2881 tagtccttgt actcgctgcc gcagtagacc accaggtcga gcgacgccgg gtcgattccc
     2941 tccagcgccc tcagcgccgc cttcgcgccc atcgcggaga cggactcctc gggcggagcc
     3001 ttgtgcacca ggttgatgcc gaacttttcc cggatgacct tctcgggaat ccccgaggcc
     3061 gccgcgatgt acgcgctccc ccggatgccg gaggggacgt agctccctat gctgcgtatg
     3121 ccgactaaat cagcggattt caaaagcgtc actcccatct agtccgtcgc cctgccgtag
     3181 agctccacca ccttcgaaac gacgatcttg cccacgttgt tcttgggcac ctcgtccacg
     3241 aaggcgatgt acttcggtat cttgtagcgg gcgagcttcg agccgagcgc cgcgacgatc
     3301 tcctctttcg agacgcgctt ccccggcttc agcgagacga cggccttgcc cacctcgccc
     3361 cacttctcgt cgggaacgcc aaagacgcag acctcgtgga tctccgcgat gtcgtacatc
     3421 gcccgctcga tctccggcgg aaagacgttc tcgccgccgc tgatgaacat gttcttcttc
     3481 cggccgacga tgtagtagta gccgtcctcg tcgcgcaccg ccatgtcgcc cgaatgcacc
     3541 cagccgtcct tcagcgtctt cgccgtctcg tccgggttgt tccagtagcc ggagaaggtc
     3601 tgcggcccgc cccacagcag ctcgccgggg gtgttcggct cggagatctc gttcccgtcg
     3661 tcgtccgcga gcttcaccat cgaaaggtac atgggcttgc ccaccgaggc gtacttcgcc
     3721 ttcatgacgt cgtcggatat gaggtgcgcg cacgaggaga tgttgttcgg gccggcctcc
     3781 gtcatgccgt atccaaggac gaacttcacg cccttgttcc agaacttctc catgatgttg
     3841 acgggcgtcg gggcggcccc cgccatgatc cacttcacgc tggagaagtc cgcggaggcg
     3901 aactccggca gctccaccat catcctgaag atcgtggccg cgccgaagag cgtggtgatc
     3961 cgttcctttt cgacgatctc gagcgtcttc ttcggatcga actgcctgtt gatgaccagc
     4021 cgtccgccga cgtgaagcag cggcagggtc agcaggttcc acccgccggt gtggaagagg
     4081 ggaagcagca gcagcgtgct gtcctcgtgg tgcaggttcc aggtacagat ctcgttgagg
     4141 cagttgaaca gctcgcagcg gtgcgagatc agcccgccct tggggaggcc cgtcgtcccg
     4201 ccggtgtgga taatcatgtg gatatcttcg atatcaagct cgtcgcagcc gacgaacgcg
     4261 tcgttcccgt gccccgtcgc ctcggcgtag gtcgcgtcgt tctccgggtt cttcccgggg
     4321 gagaggacga ggtacttctc cacggagcag cccgacttca gatgcggtat cttgtccgcg
     4381 aagacctcct cgtagaagag gaccttcggc gtttcgttgt tgacgagctg caccagttcg
     4441 ctcaccgaga ggcgcgcgtt gtacggaacg aagatcgcgc ccagcttgcc cgtggcgtag
     4501 tacgcgtcga tcatctcgat gcagttccgc gcgatgaagg cgacgcggtc gcccttgcgc
     4561 actccggcct gatcttttaa gtagcgcgcc agcaggttcg cccgccgttc gaggtcgccg
     4621 taggtgtagc gcgtctccgt gtcgaggtcg tagaccgcct cccgcgaact gtcgatgcgg
     4681 gcgcggaaga acgagtagtt gcctatccag ctgttcggca tattcagccc cccttacagg
     4741 cgcttcagca gggcgacgac cctgtccgcg atattcgtca tgataccgac gccctcttcg
     4801 tcctcctcgg cgttcttcgc gccgcccttc ccggcgaccc ccacgttcca gtaggtgttg
     4861 ccgacgatga tgcagtcgtt cgccgcagcc cagagcagca tctgcgcgaa ggcgaagttg
     4921 tgccccgccc tgcgggcggt gctcaccggc acgacgacct ttccggagag cgcggtcgtt
     4981 ccccaggtat agctctcgcc ttcctttttc atctcgttct tcgcccagcg gccg
//
LOCUS       DAOSWW010000067         5026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11205_length_5026_cov_3.606115, whole genome shotgun sequence.
ACCESSION   DAOSWW010000067 DAOSWW010000000
VERSION     DAOSWW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11205_length_5026_cov_3.606115"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PK442_02285"
     CDS             complement(<1..553)
                     /locus_tag="PK442_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOO86507.1"
                     /translation="MKRPLRRVLCATLFALLLAAGGGLLWKASLPLPIDEVLEWPTSP
                     VLLDRRGEVFSVFLSPESEWSVPVSLEQMGRWLPFVAVEVEDRRFMEHGGIDYAALLR
                     AIWQNVSAGKIISGASTITSQLVRLSFPRPRTLKTKLVEFTAAQKLEEMLTKEEILEL
                     YLNRAPFGGNIRGVEAASRIYFSK"
     gene            complement(556..4995)
                     /locus_tag="PK442_02290"
     CDS             complement(556..4995)
                     /locus_tag="PK442_02290"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOO86508.1"
                     /translation="MTIKKGLPSKDGKALEQEFKRAVIFPDYYPSLAFPSGGTFLSPA
                     GDLRIPIESVNIEEITLNLWRLYENNIPVSMTMSNYSIPRDLARRVATRTARPEGDLN
                     ETVRRAIDLRELAGEAKGVFLLTASESSGEYWADTEQLVAVTDLGIVARVWANGITVW
                     VNSILGIEPVKDATVRVYSKSNQLLASGSTDENGLWTKQGAEPWDDQLLPAIVTVEKG
                     DDISFLKFESNLLADTGFDIDGRPWTDKYDALLFAPRGVFRPGENVDFSALVRTKDLL
                     PPEPFPVVYVVRSSLGREVARGTALLSSEGIASFSARLAPASPTGTYSAVIALPGGEN
                     APLGRTEFFVEDFVAPRLEVKAVPDASFLTAGDTLTIDVSSSYLFGAPASGLVFEGEV
                     RAVAAPFRPEGWNAFTFGDMERTFESMTDFVGSGTLDETGKGTLSFSGPEGWLPPAAI
                     DLSFFVRVMEESGRWVPTSVRLPFHPCPVYLGIETPKGDVVPGKEIAVRVAAVTPEGK
                     ASDLEIAVAEVFLVQRHYNLVRTGNQTRMQMQRELVPHAQGGVEFADGIGSFSFTPKI
                     DGEYVVKISDETSNSMASSTLYAWTPYGGGASGSTLLDRVVVTADKKSYEPGEKATVT
                     LRSPFEGNLLVTVETNRELFRKVVSLKKGEVNLQVPVTDDMIPNAYITASVIRPVVEG
                     EEWGNHRAIGTLSIPVERKEYHLDIALLSPERTLPDEPLKVNGTLTDGSGAPRKGEVV
                     LFLVDEGILSLTGYRTPDPWAFFMAKRAQGVSVYDLYDQLLPLESRETPLLKAGGGAG
                     EEAMAALRAGMSPVSARTFRMLSLFLGSVPTDENGAFEATFDVPEFSGKGRLMAVASA
                     GKSFGKGEKFVAIARDITTELSLPRAVSPGDTFHAPLKIFSSASGEKQVSVKVGIDGP
                     LALTGKKEFVANLAAGGEALFSVPFKAGDEAGTTYLTVETQWEDGSFTQNLDLPVRPA
                     FPRIALSGSGVVRGGGQGSIEIPRDWFPGTEEGRLLLSDLPMLDLAGAASFLLQYPYG
                     CLEQTVSGAWPLLVLGDIVRDVDPLLVNEEEQKSALALRIRQISAMQLYSGGFAMWPG
                     SSIPSMWGSLYAAHFLVEAKKGGAPVPDDLLDSALANVRSMLPLAPANESDVAYRENM
                     TLKAYAAYILALRGEPPLGWMAHLNEHSDALRESGKIFLAGAYALASKTSEPLRKLGT
                     GAPSLRSDETTTFESASRNNALKLLMWSEVDPLSATASDLALKLIEEARSSTWRTTQD
                     NGMAVLALGRWMEKTRGARKPFTALVKDTSGATVASFTDGKRVSLSLKEVPQGPLTLS
                     LDGEGTAYFAWTSAGVPMKAPKPSSNGIALKRTWSGRDGKPLAEGAPLSRGDRITATL
                     TMTPSTALSDLVVVDMLPGGMEIENLRLTGEGTSPDQGGIRAELRDDRLILFVDFLTK
                     PVEYKYVIRAVSRGTFTLPPLAAEGMYAPDISAVGTPGTVEIR"
BASE COUNT          934 a         1554 c         1572 g          966 t
ORIGIN      
        1 ccttcgagaa gtaaattctg gaggccgctt cgacgccccg tatgttacct ccgaacggag
       61 cgcggttcag atagagttcc agtatctcct ccttcgtgag catctcctcg agcttctgcg
      121 cggcggtgaa ttccaccagc ttcgtcttca gcgtccgcgg gcgcggaaaa gagaggcgga
      181 cgagctgact tgtgatcgtc gacgctcccg aaataatctt gcctgccgag acgttttgcc
      241 atatagcgcg gagaagcgcg gcatagtcga tcccgccgtg ctccatgaaa cgccggtcct
      301 ctacctcgac ggcgacgaac ggaagccatc gccccatctg ttcgagcgat acgggaaccg
      361 accattcgga ttccggagag agaaaaacgg aaaagacctc ccccctgcgg tcgaggagca
      421 cgggggaggt cggccattca agcacttcgt cgatcggaag cgggagggaa gccttccaga
      481 gaaggccccc tcctgccgcg agaagcagag cgaacagcgt cgcgcagagg acgcggcgga
      541 gggggcgttt cataatcagc gaatctccac ggtccccggg gttcccacgg ccgagatgtc
      601 cggcgcgtac attccttccg ccgcaagggg cggcagcgtg aaggttcccc tggaaacggc
      661 gcggatcacg tatttgtact caacaggttt cgtcaaaaag tcgacgaaga ggatcagcct
      721 gtcgtcgcgc agttccgcgc gaataccgcc ctgatccggg gacgtccctt cgccggtgag
      781 ccggaggttc tcgatctcca taccgcccgg cagcatatcg acgaccacca gatcgctcaa
      841 cgctgttgag ggggtcatcg tgagcgtcgc cgttatccgg tcgcccctgg aaagaggcgc
      901 gccttccgcg agcggcttcc cgtcacgccc gctccatgtg cgtttgaggg cgattccgtt
      961 gctcgatggc ttcggcgcct tcatcggtac gccggccgac gtccacgcga agtaggcggt
     1021 tccctcgccg tcgagcgaga gcgtcagcgg tccctgcgga acctccttca ggctcagaga
     1081 aacccgcttt ccgtcggtga acgaagcgac cgtcgcgccg gaggtatcct tcacaagcgc
     1141 ggtgaacggt ttgcgcgccc cccgcgtctt ctccatccaa cggccgagcg cgagcacggc
     1201 catgccgttg tcctgcgttg ttctccatgt gctgctgcgg gcctcttcga tcagtttgag
     1261 ggccagatcg gaggctgtcg ccgagagagg atcgacctcg ctccacatga ggagtttcag
     1321 cgcgttgttc ctcgacgctg actcgaaggt tgttgtctcg tcgctccgga gcgaaggggc
     1381 tcctgtcccg agtttgcgga gaggttccga tgttttggaa gccagtgcgt acgcgccggc
     1441 aaggaagatc tttccggact cgcggagcgc atcgctatgt tcgttcaggt gcgccatcca
     1501 cccgaggggc ggttcgccgc gcaaagcgag gatatacgcc gcatacgctt tcagggtcat
     1561 gttctcccgg tacgcaacgt cgctctcgtt cgcaggagcc agaggcagca tgctccggac
     1621 gttggcgagg gcgctgtcga gcaggtcgtc gggaacggga gcgccgccct tcttcgcctc
     1681 cacaaggaaa tgcgcggcgt acagggaacc ccacatcgag ggaatcgagc tccccggcca
     1741 catggcgaaa ccgcccgagt agagctgcat cgcggaaatc tgacggattc ggagcgcgag
     1801 cgccgatttt tgctcctcct cgttcacaag gagaggatcc acgtcgcgta cgatatcgcc
     1861 gaggacgagg agcggccatg cgcccgaaac cgtctgttca aggcacccgt acggatactg
     1921 cagaaggaag gaggccgcgc ccgcgagatc gagcatcgga agatcggaga gcagaagccg
     1981 cccctcctca gttccgggga accagtccct tggaatctct atcgatccct gtcctccgcc
     2041 gcgtaccacg cccgaacccg agagcgcgat tctcgggaag gcgggacgca ccggcagatc
     2101 gagattctgt gtgaaagagc cgtcttccca ctgcgtttcg accgtcaggt aggtcgttcc
     2161 cgcttcgtcg ccggccttga acggcacgct gaaaagcgct tcgcctccgg cggcaaggtt
     2221 tgcaacgaac tctttctttc ctgtgagcgc aaggggaccg tcgatcccca ccttcaccga
     2281 cacctgcttc tcgccggacg ctgaggagaa tatcttcagc ggagcgtgga acgtgtctcc
     2341 gggggagacg gcgcgcggca acgagagttc cgtggtgatg tcgcgcgcta tcgcgacgaa
     2401 cttctctccc ttgccgaacg attttccagc ggaggcgaca gccatgagcc gtcctttacc
     2461 gctgaactcg ggcacgtcga acgtcgcctc gaaagcgccg ttttcatcgg tcgggacgct
     2521 cccaaggaag agcgagagca tcctgaacgt ccgtgcggag accggggaca ttcccgcgcg
     2581 aagcgccgcc atggcctctt cgcctgcgcc gccgcccgcc ttgaggagcg gcgtctctct
     2641 tgattccaac gggagcagct ggtcgtacag atcgtagacc gaaactccct gcgcgcgttt
     2701 cgccatgaag aaggcccacg ggtccggcgt cctgtaccct gtgagcgaga ggattccctc
     2761 gtctacaagg aagaggacca cctcgccctt gcggggcgct cccgaaccgt cggtcagcgt
     2821 gccgttcact ttcagcggtt cgtccgggag cgtgcgttcg gggctcagca gagcgatgtc
     2881 gagatggtat tccttacgct ccaccgggat ggagagcgtg ccgatcgccc ggtggtttcc
     2941 ccactcttca ccctcgacga cgggccggat cacggaagcc gttatatagg cgttcggaat
     3001 catgtcgtcg gtgacgggaa cctgaagatt cacctcgcct ttcttcagag agacaacttt
     3061 tcggaaaagt tccctgtttg tttcgacggt aaccaggagg ttcccctcga aggggcttcg
     3121 gagcgtgact gtcgcctttt ctcccggttc gtagctcttc ttgtccgcgg tcaccacaac
     3181 gcggtcgagc agcgtggagc cgcttgcgcc gccgccgtag ggagtccaag cgtagagcgt
     3241 cgacgacgcc atgctgttgg acgtttcgtc ggagattttg acgacgtact cgccgtcgat
     3301 cttcggtgtg aaggagaacg agccgatacc gtccgcgaat tccacgcccc cttgagcgtg
     3361 cgggacaagt tcccgctgca tctgcatcct cgtctggttg cccgtacgga cgaggttata
     3421 gtggcgctgg acaaggaaaa cctcggcaac tgcgatttcg agatccgatg ctttgccctc
     3481 cggcgttact gccgcaactc ggaccgcgat ctccttaccg ggaacgacgt cccctttggg
     3541 cgtctcgata cccaagtaga cggggcaggg gtggaagggg agtctcacgg aggtcgggac
     3601 ccatcgtccg ctttcctcca tcaccctgac gaagaatgag agatcgatag ccgccggggg
     3661 aagccagcct tcaggtccgg agaaggagag cgttcccttc cccgtctcgt cgagtgtgcc
     3721 ggacccgacg aagtccgtca tgctctcgaa cgtgcgctcc atatcgccga aggtgaacgc
     3781 gttccaccct tcggggcgga acggcgctgc cacggcacgc acctcaccct cgaagacgag
     3841 tccggacgca ggagcgccga agagatacga cgaggagacg tcgatggtca gcgtatcccc
     3901 agcggtcagg aaggaggcgt cggggacggc cttcacttcg aggcggggag cgacgaagtc
     3961 ctccacgaaa aactcggttc ttccaagagg cgcgttttct cctccgggaa gggctatcac
     4021 cgcgctgtac gtgcctgtgg gggaggcggg cgcaagacgg gcggagaagg aggctatccc
     4081 ttcggaagag agcagcgcgg ttccgcgcgc gacttcgcgc ccaagagacg aacgcacgac
     4141 gtacaccacg gggaacggct ccgggggcag caggtctttt gtccgcacga gcgcggagaa
     4201 gtccacgttc tcgcccgggc ggaagacgcc cctcggcgcg aagagcaacg cgtcgtactt
     4261 gtcggtccag gggcgtccgt cgatatcgaa gcccgtatcc gccaaaagat tgctctcaaa
     4321 cttcaggaat gagatgtcgt ctcccttttc gaccgtcacg atggccggaa gaagctgatc
     4381 atcccaaggt tcggcgccct gtttcgtcca cagaccgttt tcgtcggtgg aaccgctcgc
     4441 aagaagctgg ttgctcttcg agtagacgcg aaccgtcgcg tctttgacgg gctctatccc
     4501 gaggatggag ttcacccaca cggtgatccc gttcgcccag accctggcga cgatgcccag
     4561 gtcggtcacg gcgaccagct gttcagtgtc cgcccagtat tcgcctgaag actcggacgc
     4621 cgtcagcagg aagacgccct tcgcctctcc ggccagttcg cgcaggtcga tggcgcgccg
     4681 cacggtttcg ttgaggtccc cctcggggcg cgcggtgcga gtggcgactc tccgggcaag
     4741 gtctctgggt atggagtagt tcgacatcgt catggagacc gggatgttgt tctcgtacaa
     4801 acgccacagg ttgagcgtga tctcctcgat gttcactgat tcaatgggaa tccgcagatc
     4861 gcctgcggga gagaggaatg ttccgcctga cgggaaggcg agggatggat agtagtcggg
     4921 gaagatgacc gctctcttga actcctgttc aagagctttg ccgtctttcg aaggaagtcc
     4981 ctttttgatg gtcaattcca ctcgctgttt gggcttgaag tcgccg
//
LOCUS       DAOSWW010000068         4997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11295_length_4997_cov_3.236746, whole genome shotgun sequence.
ACCESSION   DAOSWW010000068 DAOSWW010000000
VERSION     DAOSWW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11295_length_4997_cov_3.236746"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PK442_02295"
     CDS             complement(<1..340)
                     /locus_tag="PK442_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOO86509.1"
                     /translation="MNAAGRSTGDAYGHALISIAASDERIVALEGGDAAWSSPFSESY
                     PERCFNAGGAEQNLILTAAGLALGGDNVFVSSYAAFLIGKGYDQIRTAVAIPSLPVRI
                     AGLGAGLTAGE"
     gene            complement(337..1170)
                     /locus_tag="PK442_02300"
     CDS             complement(337..1170)
                     /locus_tag="PK442_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOO86510.1"
                     /translation="MLREPSFLAEKASEIRRDVVRMIGLARSGRLASSLSIVDILAWL
                     YWDVLSVDPADPWFESRDRFVLSKGRGAPALYATLANRKFFERDELWSYRRLGAMLQG
                     YPQALRIPGVDAPGGGSFGMGLGIANGLAIALRRGRSPCRVFCLAGEEELQEGAFWEA
                     VIAASRFCLDSICLVIDRYRTKEEAGIPSASDEAFCEKIRSFGWTAEACDGHDFSSLG
                     GALRLLGQGAGRPKCLLARTRLGKGVSFLEGDSSGESEVLDRERVDKALRELEKEEDP
                     L"
     gene            complement(1175..2212)
                     /gene="csaB"
                     /locus_tag="PK442_02305"
     CDS             complement(1175..2212)
                     /gene="csaB"
                     /locus_tag="PK442_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOO86511.1"
                     /translation="MRPEREFRVALCGYYGFGNLGDELLAEALIAALERCGIKRGEMA
                     MLSASPEESARAHGVCSVSRWSPRAVFRILRRSETFLLGGGGLFQDVTSLRSPLYYWG
                     ALRLALLAGARPWCAGQSVGPLLTKRGRLLARSALGSCSVRGVRDARSAELLADWGMN
                     SVVTCDPVFSLTPPVTSPRHGSALLVNIRPWTGGLASKTAEESARLASSRSLSLTGFA
                     LAAEDAGVMEELRRSGVFPAERIVLLDAENWRSECGRLFPEADGVVAMRFHASLLALL
                     FGRPLTAVAYDPKVESLASEWSLPSWSGSGALPEPAAPLGPFRPFEKGAEFLKTFSEM
                     CAAAMRRDGKE"
     gene            complement(2209..4182)
                     /locus_tag="PK442_02310"
     CDS             complement(2209..4182)
                     /locus_tag="PK442_02310"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOO86512.1"
                     /translation="MIKSTADFLETSRRNPAIFVALVLILLSAWALLPRLAAERESKD
                     AAIVLDYRDLATFSIREGKSPEMLWPLLSQNGASGLMVSELTGQQLSMGTLPLYYGPA
                     SGLPGAAVKLASGNPLRAAFFIPRTHPGAALLPPLLSARFRGAHVQEYETGTAVLLTR
                     SLEELFFTGVLPDIEGLLIAAKHDIPVFYRVAPALPSDTDASLATLGALLAAFPQIRT
                     VSPSGEVALGYLNLRPLAERIVKEGRSVAMVEFSRQIGAPQLNWLAYPRLVSLHSVTN
                     EEISSRNISRSALYERFVRAVKERAVRLLVFRPSAMEASDAPFERFIEELRTLASGIT
                     ASGAQLRWPEPFQPWRTGFVGALALALAFVFSMLRFLQRFFNGGTDDRKNAEDALAWR
                     TTPRGLAVFSAIVLLAGVIILLLPSAGRFAGALTAVFVVVEASFLALDGWRTPWLGII
                     GSFLFAVAGGLAIAAFFSDPASMLRLRSFSGVKATLFLPPLLVLLADLKRRVHPESLG
                     ELFRRPPLWGEIFLLVLLLGGAGLVLFRSDNVQFVPAFEVRMREFLERALVARPRNKE
                     VFLGYPALLLWYFVRKADMWPRYREVFRLATVIGFSSAVNSFCHFHTPLFFILFRQFN
                     GLWTGVLLGMISIVFLRFVIMPLWYRYGGLVTE"
     gene            complement(4182..4778)
                     /locus_tag="PK442_02315"
     CDS             complement(4182..4778)
                     /locus_tag="PK442_02315"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOO86513.1"
                     /translation="MGGSVNLVLASGSPRRRELLHSLGWSFRIDVPDICEDLLPGEGP
                     EEAARRLSRAKAAEAAARNPGALVVAADTLVAAGGVILGKPSCREESLRMLRMLSGAV
                     HSVFTGVALRRGEEERFAVEETRVEFRPLSEESMKLYVESGEGDDKAGAYAIQGRGAL
                     LVSSLHGDYFNVVGLPLCRLSRLLEEFGCSLADQWRNS"
     gene            complement(4763..>4997)
                     /locus_tag="PK442_02320"
     CDS             complement(4763..>4997)
                     /locus_tag="PK442_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86514.1"
                     /translation="GSSVKWWIIVASLIIGTISGLYLQRFALTEPFFRDILSTGFSLT
                     DVHLGFVDFGLKVWLRWNLGTIIGGVVGIWVAR"
BASE COUNT         1179 a         1614 c         1430 g          774 t
ORIGIN      
        1 cttcgccggc cgtaagccct gctccgagcc ccgcgatgcg caccgggagc gagggaatgg
       61 ccaccgccgt ccgaatctga tcgtaccctt tgccgatgag aaaggccgca taggatgaaa
      121 cgaaaacgtt atcccctcca agagcgagcc cggctgccgt cagaataaga ttctgttccg
      181 cgcctccggc attgaaacag cgctccgggt acgattctga aaacggcgag ctccacgccg
      241 cgtctccgcc ttcaagcgca acgatcctct cgtcggaggc ggctatggag atgagcgcgt
      301 gtccgtaggc gtctccggtg cttcttccag cggcgttcat agcggatctt cctccttctc
      361 aagctctctg agggccttat ccacacgctc ccgatccaga acttcgcttt cgccggagct
      421 gtcgccttcg agaaaggaga cccctttgcc caggcgagtg cgtgcgagca gacacttcgg
      481 acggcccgcc ccttgcccga ggagccgcaa cgcccccccc agagaggaaa aatcgtgtcc
      541 gtcgcacgct tccgccgtcc agccgaagga gcggatcttt tcgcagaacg cctcgtccga
      601 cgcagacggg attccagctt cttccttcgt cctgtacctg tcgataacga ggcagatact
      661 gtcgagacag aagcgggagg ccgctatcac agcttcccag aaggctccct cttgcagctc
      721 ctcttctcct gccagacaga agaccctgca gggcgaccgc ccgcgccgga gcgcgatcgc
      781 cagtccgttg gcaattccca atcccatgcc gaaagacccg ccgccggggg cgtcgacgcc
      841 gggaatgcga agcgcctgcg ggtacccctg aagcatcgct cccagacggc ggtagctcca
      901 cagctcatcg cgctcgaaaa acttcctgtt ggcaagcgtc gcgtaaagcg caggcgctcc
      961 gcgccccttg ctgagcacga atctgtcccg ggactcaaac cacggatcgg ctggatcgac
     1021 ggagaggacg tcccagtaga gccaggcaag tatatccact atcgaaagag aagaagccag
     1081 acgccccgaa cgggccagac cgatcatacg tactacatcg cgccggatct ccgacgcttt
     1141 ttccgcgagg aaggacggtt cacgaagcat accttcactc ctttccatcg cgtcgcattg
     1201 ctgcggcgca catttcggag aaagttttca ggaactccgc gcccttttca aaaggccgga
     1261 acggacctaa cggcgccgca ggctccggaa gagccccgga gccgctccac gaaggaagcg
     1321 accattcgga ggcaagagac tccaccttcg ggtcgtaggc gaccgccgtc agaggcctgc
     1381 cgaagagcag cgcaagaagc gacgcgtgga agcgcatcgc aaccactcca tcagcttccg
     1441 gaaaaagacg gccgcattcg ctcctccagt tctccgcgtc gaggaggaca atacgctccg
     1501 ccggaaaaac tccgctgcgg cgaagctcct ccatgacgcc ggcatcctcc gccgcaaggg
     1561 caaaccccgt gagggagaga gaacgggaag acgccaggcg agcagattct tccgcagttt
     1621 tgctggcgag tcctcccgtc cacggacgga tgttcacaag caacgcgctt ccgtgccggg
     1681 gagaggtaac cggaggagtc agcgaaaaga ccggatcgca cgttacaaca gagttcattc
     1741 cccaatcggc cagaagctcc gccgaccggg catcccgcac accccggacc gagcagctcc
     1801 ccaatgccga ccgcgccagc agtcttcctc tcttcgtcag caacggccct acggactgcc
     1861 ctgcgcacca ggggcgcgcc cctgcgagga gagcaagccg gagcgctccc cagtaataga
     1921 gcggcgaacg aaggctggtg acgtcctgaa acagaccgcc gccgccgagg agaaacgtct
     1981 cgctccgccg aagaatccgg aatacggcgc gcggcgacca gcgggagaca gagcagaccc
     2041 cgtgagcgcg cgcagattcc tccggggagg cggagagcat cgccatttcg ccgcgcttga
     2101 ttccgcaccg ttcaagggct gcgatcaatg cctcggcgag cagttcgtct ccgagattcc
     2161 cgaatccgta atacccgcag agcgcaacgc ggaactcccg ttccggcctc actccgtcac
     2221 caaccctccg tacctgtacc agagaggcat gataacgaag cgaagaaaga cgatacttat
     2281 cataccgagc agcacccctg tccacaatcc gttgaattgc ctgaacagaa taaagaagag
     2341 cggagtgtgg aagtggcaga agctgttcac agccgaagaa aaaccgatga ctgtcgccag
     2401 gcggaagacc tcgcggtagc gaggccacat atccgccttg cggacgaaat accagaggag
     2461 gagcgccggg tagcccagga aaacctcttt gttccgcggt cgggccacaa gcgcccgttc
     2521 caggaattcc cgcatacgga cttcgaacgc ggggacgaac tggacattat cgctgcggaa
     2581 gagaacgagt ccggcgcctc ccagcaaaag aaccagaagg aatatctcac cccacagcgg
     2641 cggccttcga aagagctctc ccagagattc gggatgcact ctccgcttca gatcagccaa
     2701 aagcacgagc aggggaggca agaagagcgt cgctttcacc ccggaaaagc tcctcaaccg
     2761 aagcatcgac gcaggatcgc tgaagaaagc cgctatggcc aaccctccgg cgactgcaaa
     2821 aaggaaactc ccgataatgc cgagccacgg agtcctccag ccgtcaagag cgaggaaaga
     2881 cgcctcgacg acgacgaaga cagcagtcag ggctccggca aagcggcctg cggaaggaag
     2941 aaggagaatg atcacacccg cgaggagaac aatagcggaa aagacagcga gccctctcgg
     3001 cgtcgtccgc catgcaagcg cgtcttcagc gtttttccgg tcatcggttc cgccgttgaa
     3061 aaacctctga agaaagcgga gcatagaaaa gacaaaggcg agcgcaaggg caagggcgcc
     3121 gacgaatccc gtccgccatg gctggaacgg ctccggccag cggagttgcg cgcccgacgc
     3181 ggtaataccc gacgccaggg tacgcaactc ttcaataaac cgttcaaacg gagcgtcgga
     3241 cgcctccatg gccgacggcc ggaagacgag aaggcgaacc gcgcgctctt tcaccgcccg
     3301 gacgaaacgc tcgtacaatg cactacggga gatgttgcgg ctcgaaattt cctcgttggt
     3361 cacgctatgc agcgaaacaa gccggggata cgccagccag ttcagctggg gagcgcctat
     3421 ctgccgggaa aattcgacca tcgccacgga acgcccctcc ttgacgatac gttcggcaag
     3481 ggggcgaaga ttcaggtatc caagagcgac ttcgccggaa ggggagacgg tgcgaatctg
     3541 ggggaaggcc gctaaaagcg ctccgagcgt cgccagagaa gcatccgtat ccgacggaag
     3601 cgcaggcgca accctgtaga aaacgggaat atcgtgcttt gccgcaatca gcagcccttc
     3661 gatatcgggc agaacgccgg taaagaagag ttcttcgaga ctcctggtga gcagcaccgc
     3721 cgttcctgtc tcgtactcct gaacgtgcgc ccctctgaat ctggcgctca aaagcggggg
     3781 aagaagggct gccccgggat gtgttcgagg aataaaaaag gcggcccgta acggattccc
     3841 ggatgcaagc ttcactgcgg ctcccggaag gccggaggct gggccgtagt agagagggag
     3901 cgtccccata gagagctgct gccccgtcaa ctccgacacc atcagcccgg aagcgccgtt
     3961 ttgcgaaaga agcggccaga gcatctccgg gctctttccc tctctgatcg agaaggttgc
     4021 caggtcgcga tagtcgagaa cgatggcggc gtctttcgac tcccgttccg ccgccagccg
     4081 gggcagaaga gcccaggcgg agaggagaat gagtacaaga gcgacaaaaa tcgcgggatt
     4141 ccgccgcgaa gtttcaagaa aatcagctgt ggatttgatc atcacgagtt cctccattga
     4201 tcggccagag agcagccgaa ttcttcaagc aaacggctca gtctgcaaag cggcagccca
     4261 accacgttga agtagtcgcc gtgcaacgag gacaccaaca acgcgccccg cccctggatc
     4321 gcgtaagcgc ccgccttgtc atccccctcg ccgctttcga cgtagagttt catcgactcc
     4381 tccgaaagag gccggaactc caccctggtc tcctcgacag cgaaccgctc ctcttctcca
     4441 cggcgaagtg cgactccggt gaagacgcta tgaaccgccc cgctgagcat ccgaagcatc
     4501 cgaagcgatt cctcccggca ggacggtttg ccgaggataa cgccgcccgc ggcaaccagc
     4561 gtgtcggcag ccactacaag cgcccccgga tttcgtgccg ccgcctcggc cgcctttgcc
     4621 cgcgaaagac gcctcgcggc ctcctcggga ccttctccgg gaagcagatc ctcgcaaata
     4681 tccgggacgt ctatccggaa agaccacccc agggaatgaa gcaactcacg ccgcctgggg
     4741 ctccccgacg caagcacaag atttaccgag ccacccaaat tcccaccacg cctccaataa
     4801 tcgtgcccaa attccatcga agccagacct tcagtccgaa gtcgacaaat ccgaggtgca
     4861 catcagtaag actgaaaccg gttgaaagga tatccctgaa aaagggctcc gtcaaggcaa
     4921 agcgctgaag atagagtcct gaaatagtac ctatgatgag cgaagcgacg atgatccacc
     4981 acttcacgga agagccg
//
LOCUS       DAOSWW010000069         4996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11297_length_4996_cov_3.993726, whole genome shotgun sequence.
ACCESSION   DAOSWW010000069 DAOSWW010000000
VERSION     DAOSWW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11297_length_4996_cov_3.993726"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /gene="miaA"
                     /locus_tag="PK442_02325"
     CDS             <1..865
                     /gene="miaA"
                     /locus_tag="PK442_02325"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOO86515.1"
                     /translation="VYRYLDIGADKISPDIRRRTIHHMIDVADPDQVFTAADFVSGCV
                     DAVERIRARGRIPLFAGGTPFYFEALFGGMLSEELPKDEDLRRELEQIAAEQGAGALH
                     ATLRSVDPESAGRLHENDVRRVVRALEICRLTGKTVAEAWSERKKMTPPKEYDVLYVG
                     LTRERRFLFESIERRVGEQFASGFVEEVEWLLNNGYDERFPSMQGFGYKDILEYLRGR
                     CSREEAAERDIRQTKAFSRRQMTWFGKFSPILWYDTVDCSMTKLVDTIENDVRHYPGR
                     WSFVNGAQEGN"
     gene            843..1763
                     /gene="ispH"
                     /locus_tag="PK442_02330"
     CDS             843..1763
                     /gene="ispH"
                     /locus_tag="PK442_02330"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOO86516.1"
                     /translation="MEHKRVIETASPTGFCFGVQRAIESLEKRIEECASSASGSDGRV
                     YSIGMPIHNPQEVERLSRKGLRVVDRIEDIPCGSAVFVRAHGVPPSVKEELISRCGAE
                     NVTDATCPFVKNAQDKAAFLAGEGYSVLVLGDPGHPEVQAIVGCAGGDVLVASSLSEV
                     KALGKRDRLGVISQTTQKGEFLGSAASILAPQIRELRVFNTICGATTQRQEAVREITS
                     RVDGLLVVGGRNSANTAKLVEIARSRNCDVLWIEHAGELSGEWFSGKHKIGIAAGAST
                     PDWLIEEVKHAIETSQVSRGMEGYDGRNDE"
     gene            1741..3303
                     /locus_tag="PK442_02335"
     CDS             1741..3303
                     /locus_tag="PK442_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOO86517.1"
                     /translation="MTEEMMNEGLRTTDVENAEGREVEETGEAQETMESILEQYGEVE
                     ELHRGKIVTGTVVNVVDGGWLVDVGYKCEGFLPAKEWSHRVLVEDEAEPAIEEKIQIQ
                     VVSIRHGEEAQLLVSRWRCEFDRRWSELESKLAQNEVIPVKGIRKVKGGLMVECCGLE
                     GFVPISHLAEEGRGVNPGKFVGEVFDAKLMEKDKKKHRLVFSRRMIVEETLVAERESF
                     YTDVAEGSVLDGEVSSITSFGVFVNVGPIDGLVHMSELSWKRNIKPKEMFKKGDPVKV
                     RVIGIDRENNRVSLSIKQTENDPWTTASSKWKKNEVTKGVVTNVTDFGAFVEVEPGIE
                     GLIHIGDLSWTRIKHPRDVLRKGQEVEVQVLDVDMEKKRMSLGFKQLNDPWNDIATRY
                     SKDQDLTVKVVRLADFGAFVEIEEGVEGLIHISQLSTKRVEKPGDVLSEGQEVLARII
                     EINPSDRRMRLSISALEEPAGESSARPRDEERRRGKQERGDHDRHPQKNVMSDGEANV
                     TIGEFLKNSLQD"
     gene            3371..3889
                     /gene="def"
                     /locus_tag="PK442_02340"
     CDS             3371..3889
                     /gene="def"
                     /locus_tag="PK442_02340"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOO86518.1"
                     /translation="MNSLRLRVYPDPVLRKETEPVVAFDDDLKDLVAEMIRLMNLHDG
                     VGLAAPQAGVSSRLAVVFHDGMLHVLANPVLLSSEGEQDGEEGCLSFPGIFGNVQRPS
                     KITIRHNDLDGVEHETSVEGFLARAFLHEMDHLDGRLLIDNFSPMRRAMARKKLLRSK
                     EGEDADGGSSRR"
     gene            3886..4833
                     /gene="fmt"
                     /locus_tag="PK442_02345"
     CDS             3886..4833
                     /gene="fmt"
                     /locus_tag="PK442_02345"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOO86519.1"
                     /translation="MKDAFQRNPVLWFFGTGTFAASCLKHLVKNVHFDCIVTSPPSSG
                     GRGMRNILSPVETLCETLHLHVIRSSKVSEEPELLHHLAESPPDCILVVDFGQKILEP
                     FLSAPAWGCLNIHPSLLPSYRGAAPVPRALLNGDAVTGVSLFRLVEEMDAGPILFRKE
                     LTVGETASAGEILEILAEKGSELYLLGVKCLIEGSCKFRDQNSESATYAPKIANKEAE
                     CSWVWPSHRLVSTVRAMNPSPGAYIFIGAKRMKIWEVLHHPSLGRPGEIVDFLDGFPL
                     VGTSDGSVALLCVQPEGKKRLSGAEWARGIRLKKGDFLQ"
BASE COUNT         1155 a         1262 c         1545 g         1034 t
ORIGIN      
        1 ggtttacagg tacctggata tcggcgcgga caagatttcg ccggatattc gacggcgaac
       61 tatccatcac atgatcgacg tggcggaccc ggaccaggtg ttcaccgcgg cggacttcgt
      121 ctcaggctgc gtggacgcgg tggaaagaat acgcgcgcgc gggcggattc ctctctttgc
      181 gggaggcacg cccttctact tcgaagcgct cttcggcgga atgctctccg aggaactgcc
      241 caaggacgaa gatctgagga gagagctgga gcaaatcgcc gcggaacagg gagcgggcgc
      301 gctgcatgcg acgcttcgtt cggtcgaccc tgagagcgcc ggaaggctcc atgagaacga
      361 tgtccggcgg gtcgtccgcg cgttggagat ctgtcgtttg accggaaaga ccgtcgcgga
      421 ggcgtggagc gaaagaaaaa agatgacgcc gccgaaagag tatgacgttc tgtacgtcgg
      481 cctgacgcgc gaacgccgct ttctcttcga aagcatcgaa cggcgggtcg gagagcagtt
      541 cgcctctggg tttgtagaag aggtggagtg gcttctcaac aacggatacg acgagcgttt
      601 tccgtccatg caggggttcg gatataagga tattctggag tatctgcggg ggcgctgctc
      661 gcgcgaagag gcagccgaac gggatatacg gcaaacaaaa gcattttctc gaaggcagat
      721 gacctggttt gggaaatttt ctccaattct atggtatgat actgtagatt gttcgatgac
      781 gaaactcgtc gatacgatcg aaaacgatgt acggcactat cctgggagat ggtctttcgt
      841 caatggagca caagagggta attgagactg cgtcccccac cggtttttgc ttcggtgttc
      901 aacgggccat agaaagtctg gagaaacgca ttgaagagtg cgcttcctcg gcttccggaa
      961 gcgatggaag agtctattcc atcggaatgc cgatccacaa tcctcaggaa gtggagcgtc
     1021 tttcccggaa agggcttcga gttgtcgacc ggatagagga tattccatgc ggctccgctg
     1081 ttttcgtccg tgcgcacggc gttcctccct ctgtgaagga ggagctcatt tcgcggtgcg
     1141 gcgccgagaa tgtcaccgac gcgacctgtc cgttcgtgaa gaacgcgcag gacaaggccg
     1201 cttttcttgc gggcgagggg tactcggtgc ttgtgctcgg cgatcccgga caccctgaag
     1261 tgcaggccat cgtggggtgc gcggggggcg acgttctggt cgcatcctct ctttccgagg
     1321 taaaggctct tggcaaaagg gacaggttgg gagtaatatc tcagacgacc cagaagggcg
     1381 agttcctcgg atccgccgcc tccatacttg cgccgcaaat ccgcgagttg cgggtgttca
     1441 acacgatatg cggcgccacg acgcagcgtc aggaggccgt gcgggagatt acctcgcggg
     1501 tcgacggact gctcgtggtc ggaggccgaa acagcgccaa cacggcgaag ctcgtcgaaa
     1561 tagcccggtc gcgcaactgc gacgtcctgt ggattgaaca tgcaggggag ctttccggcg
     1621 agtggttctc agggaaacac aaaattggaa tagcagccgg cgccagcaca ccggactggt
     1681 tgatagaaga agtgaaacac gcaatagaga catcgcaggt gtcaaggggg atggaaggtt
     1741 atgacggaag aaatgatgaa tgaaggactc cgtacaacgg acgtcgaaaa cgccgaaggg
     1801 cgcgaagttg aggaaacggg cgaagcacag gagacgatgg agagcatcct cgaacagtat
     1861 ggagaggtcg aggagcttca taggggaaag attgtcaccg gtacagtggt gaacgtcgtc
     1921 gacggcggct ggcttgtgga cgtaggatac aagtgcgagg gcttccttcc cgcaaaggaa
     1981 tggtcgcaca gggttctggt cgaggatgaa gcggagcccg caatcgaaga aaaaatccag
     2041 atccaggtgg tgagcattcg tcacggcgaa gaggcgcagc ttcttgtcag ccgttggaga
     2101 tgcgagttcg accgccgctg gagcgaactt gaaagcaaac ttgcccagaa cgaagtcatt
     2161 ccagtgaagg ggatccgcaa ggtcaagggc ggtctcatgg tggagtgctg cggacttgag
     2221 gggttcgtcc ccatttcgca tcttgccgaa gagggcagag gcgtcaatcc cggcaaattc
     2281 gtgggcgagg tcttcgacgc taaattgatg gaaaaggaca agaagaagca ccggctcgtc
     2341 ttctccagac gtatgatcgt cgaggagacg ctggttgcag agcgcgagtc tttctacacc
     2401 gacgtggcgg aaggctcggt tcttgacgga gaagtaagca gcatcacatc tttcggcgtc
     2461 ttcgtgaacg tcggtcccat cgacggactc gtccacatga gcgagctttc ctggaagcgg
     2521 aacatcaagc cgaaggagat gttcaagaag ggcgacccgg tcaaggtgcg cgtcatcggg
     2581 atcgaccgcg agaacaaccg cgtttctctc agcatcaagc agactgagaa cgatccgtgg
     2641 accacggctt cttccaagtg gaagaagaat gaggtcacca agggagttgt cacaaacgtg
     2701 accgacttcg gcgcattcgt cgaggtcgag cccggaatcg aaggcctcat ccacatcggc
     2761 gatcttagct ggacccgcat aaagcatccg agagatgtgc ttcgcaaggg gcaggaggtc
     2821 gaggtgcagg tgctcgacgt cgatatggag aagaagcgca tgagtctcgg cttcaagcag
     2881 ctgaacgacc cgtggaacga tattgccacc aggtattcaa aggatcagga tctgaccgtc
     2941 aaggttgtcc ggcttgctga tttcggcgct ttcgtcgaga tcgaagaggg cgtcgagggg
     3001 ctgatccaca tctcccagct gagcacaaag agggttgaga aaccgggcga cgtgctctcc
     3061 gaggggcagg aagttctcgc ccgtatcatc gagatcaatc cttcggaccg cagaatgcgc
     3121 ctgagcatca gcgcacttga agagccggcc ggagagtctt ccgcccgccc ccgcgacgag
     3181 gaacgccgca gagggaagca ggaacgcggc gatcacgacc gtcacccgca gaagaacgtg
     3241 atgagcgacg gcgaggcgaa tgtcaccatc ggagagtttt tgaagaactc cttgcaggac
     3301 taacgtacgt caaagggtcg ccttaaaaaa ggcggccctt tttatttaca aagaaagtgc
     3361 ggcgaatata atgaattcat tgcggcttcg tgtgtatccc gacccggttc tgaggaaaga
     3421 gacagagccg gttgttgctt ttgacgatga cctgaaagac cttgtcgccg agatgatccg
     3481 gcttatgaat ctgcacgacg gggtaggatt ggcggctccg caagccggag tttcttcccg
     3541 gctcgccgtt gtgtttcacg acggaatgct ccatgttctg gccaatccgg tgctgctctc
     3601 ttcggaaggg gaacaggacg gggaagaggg atgcctgagc tttccgggga ttttcggcaa
     3661 tgtacaacgg ccttccaaga tcaccatacg gcacaacgac ctcgacgggg ttgaacacga
     3721 gacgtctgtg gagggctttc ttgcccgcgc gtttctgcac gaaatggacc atctggacgg
     3781 aaggctgctc atcgataact tctcgccaat gaggcgcgcc atggcgcgaa aaaagttatt
     3841 gcgttcaaaa gaaggggaag acgccgacgg aggttcgagc agacgatgaa ggacgccttc
     3901 caaagaaacc ccgtcctctg gtttttcgga accgggacgt ttgcggcgag ctgcctgaag
     3961 catctcgtaa agaacgttca tttcgattgt atcgtgacct cgccgccctc ttcaggggga
     4021 agaggtatgc gcaacatcct ctctcctgtt gagacactct gcgaaacact tcacctccat
     4081 gtgatccgtt cctccaaggt ttcagaagag ccggaactgc ttcatcacct cgcagagtcg
     4141 cctccggact gtattcttgt tgtggatttc gggcagaaga tactggagcc ctttctctcg
     4201 gcgcctgcgt gggggtgttt gaacatccac ccgtcgttgc ttccttcata cagaggggct
     4261 gcccctgtgc cgagggcgct tctgaacgga gacgctgtaa ccggggtttc tctcttccgt
     4321 ctcgtagagg agatggatgc ggggccaatc ctctttcgaa aagagttgac ggtaggcgaa
     4381 accgcaagcg ctggagaaat tttggagatt cttgcagaaa aaggtagcga actttatctg
     4441 ttgggtgtaa aatgtcttat tgagggaagt tgcaaattta gagatcaaaa ttcagaatca
     4501 gcaacatatg cacctaaaat tgccaacaaa gaggctgaat gttcttgggt ctggccctcg
     4561 catcgtcttg tctccaccgt tcgagcgatg aacccctctc cgggcgctta tatttttatt
     4621 ggcgcaaaac ggatgaaaat ttgggaagtc ctccatcatc catccctggg gcgccccggc
     4681 gaaatcgtag actttctcga cggatttcct cttgttggta cgtcagacgg gtctgtcgcg
     4741 cttctttgtg ttcagcctga ggggaaaaaa cgcctcagcg gcgcggaatg ggcccgcgga
     4801 atccgcctga agaaagggga ttttcttcaa tgaacgcctc tgaacgcgaa caaatcagcc
     4861 agttgatggc gtcctttcaa acatttgcct ccactcttga aaaatggccg aaggcggttg
     4921 ccgtaacagg cgcgctcggc tccggaaaga ccgaatgggt cctcaatctc gctttgggtt
     4981 ttgaagcgat cggcga
//
LOCUS       DAOSWW010000070         4988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11332_length_4988_cov_3.586053, whole genome shotgun sequence.
ACCESSION   DAOSWW010000070 DAOSWW010000000
VERSION     DAOSWW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11332_length_4988_cov_3.586053"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PK442_02350"
     CDS             <1..409
                     /locus_tag="PK442_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOO86520.1"
                     /translation="LGVPEAIVSRQPFPGPGLAVRCLGEITRERLDTLRRADAIFREE
                     LSGWEGVSSVWQSFVALLPVRTVGVMGDSRTYSEAAVLRAVESHDGMTADWVRIPWEI
                     LDRTSRRICNEVHGINRVALDVTSKPPATIEWE"
     gene            419..1531
                     /locus_tag="PK442_02355"
     CDS             419..1531
                     /locus_tag="PK442_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO86521.1"
                     /translation="MGGKGVSSGEGQKTQMSRGWFLPFLFIFLALAYLSFTVSLPLLK
                     PLAWAVLLSFTVYPVFKRMRDRFFPRSITGNWAATIATGLIVLLLVIPSLFTGFVAAR
                     EGVRFYETVADVIAGMESSGESALTAFLPEQIVERMHPWMERYPLLRTGLQQAAGWAA
                     TTAVAASRAVLGSTFTFLYYLAVIIVVSFFLIRDGHLIIEYLKDIMPLPAKESAAFFV
                     RAKQVLQAVVYGVIVTALAQGLAGAVGWRIVGLPSPVFFGALMALFAMIPFLGTALVW
                     VPGALYLFYMSETTQALILLAWGVLVVSMVDSLLRPIFISGGGKVHLLIVFIGVIGGL
                     AAWGILGLFMGPLVVTLFVFLLESYRTMWKTFFVSR"
     gene            1604..2239
                     /locus_tag="PK442_02360"
     CDS             1604..2239
                     /locus_tag="PK442_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HOO86522.1"
                     /translation="MPLLIAVSGFKDSGKTSLARALLTELSGRGLSLAFMKHTDRDVL
                     SPAGTDTGGIESQGIPCAYWGNDGLRMELPAAAPNSDTIAALFPEKDIVIVEGAKSIS
                     MPRIWVGSAESAPPEVKGVFACFDRAAEKGDGRFLYVPGEENELADRIAAFHERFANV
                     RCVRVAVRGKRLPLKPFVAEMIRGSIAGLLLPLKGIDSLKEGADIHLARSE"
     gene            2392..4413
                     /locus_tag="PK442_02365"
     CDS             2392..4413
                     /locus_tag="PK442_02365"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOO86523.1"
                     /translation="MLLFLLTLIVAVTGALALLYAYLTYSQKVLFGCTWFKVSEAPEE
                     APEGEMIEKTARVHELSEIIHKGAMAFLYREYKYLLPFLLVVGVLLIYFIGFAAGFSF
                     IIGGLCSAAAGYAGMIVATRTNGKTALAATRGMNEALLTAFRGGSVMGMSVVGIGVLG
                     IVFCYALFRDPNLITGFGMGASTIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDP
                     RNPAVIADNVGDNVGDVAGMGADLFESYVNSIIAAMALGLALFGWKGVSFPMVMAGVG
                     ILAAIVGTRFVKVEEGGNPAVALRKGTFVTGGIMIVGAFIFSFLMFGALSLFWSVLSG
                     VAVGVLIGFVTEIYTSGDYASVKHIAHASKTGPATVILEGVAVGMKSTVVPVILICAA
                     TLVSYYFAGLYGIACAAVGMLSITGMALSVDAYGPIADNAGGIAEMSHLPPEVRKITD
                     KLDAIGNTTAAMGKGLAIGSAALTALALFAAYAHSVKLTAIDLNDPKVMVGLFLGGML
                     PFLFSALSIQAVGRAAEAMIDEVRRQFREIPGIMEGTGRPEYEKCVDISTAAALKEMI
                     IPGLLAVLSPLAVGFGLGAQALGGLLAGSIVTGVMLAIFMSNAGGAWDNAKKYIEEGN
                     FGGKGTPEHAAAVVGDTVGDPFKDTAGPSLNILIKLMSVVALVLAPLFI"
     gene            4490..>4988
                     /locus_tag="PK442_02370"
     CDS             4490..>4988
                     /locus_tag="PK442_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOO86524.1"
                     /translation="MRSDDVRRVKDRLDIVEVIGDYVRLKKAGNNFLGLCPFHDEKTP
                     SFNVSPSRQTFHCFGCGQGGDVFSFIMEKENLSFREALETLAERAGVQLSSEGARTLR
                     ERSLFDIMERACSHFRSLLEKPEGEHAAAYLARRNLPREAWRNFELGWASPSWDALWK
                     ALANEG"
BASE COUNT          844 a         1478 c         1595 g         1071 t
ORIGIN      
        1 tctcggcgtg ccggaggcaa ttgtctccag gcagcccttc cccggtcccg gcctcgccgt
       61 ccgctgcctc ggagagataa cgcgcgagcg ccttgacacg ctgcgccggg cggacgccat
      121 cttccgggag gagctttccg ggtgggaagg cgtttcttcc gtatggcagt ccttcgtggc
      181 gctgcttccc gtcaggacgg tcggcgtgat gggggattcg cgcacctatt cggaggcggc
      241 ggtgctccgc gccgtggagt cgcacgacgg catgacggcc gactgggtgc ggattccctg
      301 ggagattctc gacaggacct cgcggaggat atgcaacgaa gtgcacggga tcaaccgggt
      361 cgcgctcgac gtgacaagca agccgccggc aaccatcgag tgggagtagg cgacggcgat
      421 gggagggaag ggcgtttctt ccggagaagg acagaagacg cagatgtcga gagggtggtt
      481 ccttcctttt ctctttatct tccttgcgct tgcgtacctc tccttcacgg tctcgcttcc
      541 tctgctcaag ccgctggcgt gggcggtgct gctttcgttc accgtctacc ccgtcttcaa
      601 acggatgcgg gaccgcttct tcccgcgtag tataacgggg aactgggccg ctacgattgc
      661 aacagggctt atcgtcctgc tgctcgtgat tccctctctt tttacagggt tcgttgcggc
      721 gcgggagggc gtccgattct acgaaaccgt cgccgatgtg atcgcgggga tggagtcgtc
      781 gggcgagagc gcgctgaccg cattcctgcc cgagcagatc gtcgagagaa tgcacccgtg
      841 gatggagcgg tatccgctgt tgcgcaccgg attgcagcag gcggccggat gggcggcgac
      901 aaccgccgtc gcggcatcgc gcgcggtttt agggagtacc tttacctttc tctactatct
      961 cgccgtgatc atcgtggtga gtttctttct catcagggac ggccacctta tcatcgagta
     1021 cctcaaggac atcatgccgc ttccggcgaa ggaaagcgcc gccttcttcg tccgcgcgaa
     1081 acaggtgttg caagccgtgg tctacggcgt catcgtcacc gctctggctc aaggcttggc
     1141 cggagccgtc ggatggcgga tcgtcggtct tccgagtccg gtcttcttcg gggctctgat
     1201 ggcgctcttc gcgatgattc cttttctggg gaccgctctc gtttgggttc cgggggcgct
     1261 ctatctcttc tacatgtcgg agacgacgca ggctctcata ttgcttgcat ggggcgtgct
     1321 cgtggtgagt atggtggaca gcctgctccg gccgattttc atctccggcg gcgggaaggt
     1381 acacctgctc atcgtcttca tcggcgtcat cggcggccta gccgcatggg ggattctggg
     1441 gctttttatg gggccgctcg tggtgacgct gttcgtcttt ctgcttgaga gctaccggac
     1501 gatgtggaaa acttttttcg tctcccggta acgccgccgg aacgtgccca atcatcaaga
     1561 aggaggaacg atgagtgaac ggacatatcg aaggaggcgg gccatgcctc tcctgatcgc
     1621 agtctccggt ttcaaggatt cggggaagac gtcgcttgcg cgcgcgcttc tcacggagct
     1681 ttccggacgg gggctctccc tcgccttcat gaaacatacg gacagggacg tgctctcccc
     1741 ggccggcacg gataccggcg gaatcgagtc tcaggggata ccgtgcgcct actgggggaa
     1801 cgacggcctc aggatggaac tgcctgcagc cgctccaaac agtgatacga tcgcggcgct
     1861 ctttccggag aaggacatcg tcatcgtcga gggtgcgaag agcatttcca tgccgaggat
     1921 ctgggttggt tccgcggaaa gcgcgccccc ggaagtgaag ggcgtttttg cgtgtttcga
     1981 cagggcggcc gaaaaagggg acgggcgttt tctctatgtt ccgggcgagg agaatgaact
     2041 ggcggatcga attgcggcgt tccatgagcg atttgcgaac gtgcggtgcg tcagggtcgc
     2101 cgttcgcggg aagcggcttc cgctcaaacc ctttgttgcc gagatgatcc gcggaagcat
     2161 cgcgggactg cttttaccgt tgaaagggat agattccctg aaggaagggg ctgatattca
     2221 tctcgcacgt agtgaataac gctgtttttt agaaatgaac cttgtttttt acattaagga
     2281 ttactataat gtcagttgtt gtatgccgtc cctaactcgc ctcgctgttc gacgaccgga
     2341 cggaaaaaag atggtttttt catatttttc ctattgggag gagtctgtga aatgctgctt
     2401 ttcttgttga cactcattgt cgcggtcacg ggagctcttg cgttgctcta cgcctacctg
     2461 acgtacagcc agaaggtatt gttcggatgc acctggttca aggtgtctga ggcgcccgag
     2521 gaagcgcccg agggagagat gatcgagaag actgcgcggg ttcatgagct ctccgagatc
     2581 atccacaaag gcgcgatggc cttcttgtac agggagtata aataccttct gccgttcctt
     2641 ctggtggtcg gcgtgctcct gatctacttc atcggtttcg cggcgggctt ctccttcatc
     2701 atcggcggtc tgtgcagcgc ggccgcaggg tatgccggaa tgatcgtggc cacgaggacc
     2761 aacggcaaga cggctcttgc cgctacgcgc gggatgaacg aagccctgct caccgccttc
     2821 agaggcggca gcgtcatggg aatgtcggtc gtgggcatcg gcgtcctcgg tatcgtcttc
     2881 tgctacgcgc tcttccgcga tcctaacctg atcacgggat tcggcatggg cgccagtacg
     2941 atcgcgctct ttgcgcgtgt cggcggcggt atctacacca aggcggcgga tgtcggcgcc
     3001 gacctcgtcg gcaaggtcga ggcggggatt cccgaggacg acccccggaa cccggcggtc
     3061 atcgcggaca acgtcggcga caacgtcggc gacgtcgcgg gcatgggcgc ggacctcttc
     3121 gagtcgtacg tgaactccat catcgcggcg atggcgctcg gactcgctct cttcggctgg
     3181 aaaggcgtct ccttcccgat ggtcatggcc ggcgtgggca ttctggccgc gatcgtcgga
     3241 acgcgtttcg tcaaggtgga ggaggggggc aaccccgccg tcgcgctgcg taaggggacc
     3301 ttcgtcacgg gcggcattat gatcgtcggc gcgttcatct tctccttcct tatgttcggc
     3361 gctctttcgc tcttctggtc ggtgctctcc ggagtggcgg tgggcgttct catcggtttc
     3421 gtcaccgaga tctacacgtc gggagactac gcctccgtga agcacatagc gcatgcgtcg
     3481 aagaccggtc ccgcaacggt gattctggaa ggcgtggcgg tcggcatgaa gtcgacggtc
     3541 gttcccgtta ttctgatatg cgccgcgacc cttgtcagct actactttgc aggcctctac
     3601 ggcattgcct gcgcggctgt cggtatgctt tcgattacag gaatggccct gagtgtggac
     3661 gcctacgggc ctatagccga caatgcgggc ggcatcgccg agatgagcca cctcccgccg
     3721 gaggtccgta agatcaccga caagctcgac gccatcggca acacgactgc ggcgatgggc
     3781 aaaggactgg cgatcggctc cgcggcgctc accgcgctcg cactcttcgc cgcgtacgcg
     3841 cactccgtca agctgacggc catcgacctg aacgacccga aggtcatggt cggtctcttc
     3901 ctcggcggca tgcttccgtt cctcttcagc gcgctttcga tccaggcggt cggccgcgcc
     3961 gcggaagcca tgatcgacga ggtgcggcgc cagttccgcg agatccccgg catcatggag
     4021 ggaacgggac ggcccgaata tgagaagtgt gtcgacatct ccaccgccgc cgcgctgaag
     4081 gaaatgatca tccccggcct gctcgcggtc ctctccccgc tggccgtcgg cttcggcctc
     4141 ggcgcccagg cgctcggcgg cctgctcgcc ggttccatcg tgacgggtgt gatgctcgcc
     4201 atcttcatgt ccaacgcggg cggcgcatgg gacaacgcga agaagtatat cgaagagggg
     4261 aacttcgggg gcaaagggac gccggagcac gccgccgcgg tcgtgggcga cacggtcggc
     4321 gacccgttca aggacaccgc cggcccgagc ctcaacatcc tcatcaagct gatgtcggtc
     4381 gtggcgctcg tgctggcgcc gctcttcatc taatccgaaa aagggagtac aatatctgcg
     4441 agagggggag ggcgacctcc cccttatttt ttcttcggag gcgccgctca tgagatcaga
     4501 cgatgttcgc cgcgtgaaag accggctcga tatagtggag gtcatcggcg attacgtccg
     4561 tctgaagaag gcggggaaca actttctcgg cctctgcccc ttccacgacg agaagacgcc
     4621 ctcgttcaac gtctccccgt cgcgccagac gttccactgt ttcggctgcg gtcagggagg
     4681 ggacgtcttc tccttcatca tggaaaaaga aaacctctcc tttcgggagg cgctcgaaac
     4741 cctcgccgaa agggccggag tgcagctctc ctccgagggc gcgcgtacgc tcagggagcg
     4801 cagcctcttc gacatcatgg agagggcctg ttcccacttc cgctccttgc ttgaaaagcc
     4861 cgaaggggag cacgccgcgg cgtacctcgc ccggcgaaat ctcccacgcg aggcgtggcg
     4921 gaacttcgag ctcggctggg cgtccccctc ctgggacgct ctctggaagg cgctcgccaa
     4981 cgaaggcg
//
LOCUS       DAOSWW010000071         4924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11546_length_4924_cov_3.659068, whole genome shotgun sequence.
ACCESSION   DAOSWW010000071 DAOSWW010000000
VERSION     DAOSWW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11546_length_4924_cov_3.659068"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PK442_02375"
     CDS             complement(<1..447)
                     /locus_tag="PK442_02375"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOO86525.1"
                     /translation="MAPVLKIVPEDWAEALSVCRARRPLIYAVTNYIAASFQANAILA
                     VGASPVMSPCIHEAEELARSAEGVLINTGAPSEDRELLIRNALTAAEAKRSATLLDPV
                     GYGAASFRRQLVDSLLRDFRFAIVKGNRGEISLLAGEKGVVKGVDSE"
     gene            complement(432..1244)
                     /gene="thiD"
                     /locus_tag="PK442_02380"
     CDS             complement(432..1244)
                     /gene="thiD"
                     /locus_tag="PK442_02380"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOO86526.1"
                     /translation="MSVYRGIAVSIAGSDSGGGAGIQADLKTCAALKVFGMTVITALT
                     AQNSTAVTDVWNVPANMVRAQMDALWSDFPVAAAKTGMLSSPEIIRTVVQGVRQWSVT
                     NLVVDPVMVAQSGASLIADDAVEALREELLPHALLVTPNIPEAERLAGFSIRSTEEME
                     RASRRIAELGPKAVLVKGGHLLREDSVTDVFFYKGELTLFEDRRIFTENTHGTGCTLS
                     AAITAELAAGIPLEEAVRRGRQYLRMGLEASFRPGKGWGPLGHAVTPPWLRS"
     regulatory      complement(1308..1411)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1436..2116)
                     /gene="pssA"
                     /locus_tag="PK442_02385"
     CDS             complement(1436..2116)
                     /gene="pssA"
                     /locus_tag="PK442_02385"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOO86527.1"
                     /translation="MVTSGNLLCGTMSLILVLHGRVVPAAWLVFMAVFFDFMDGKVAR
                     RLGGGTRFGLEFDSLADVVSFGVAPAVLFYSVYAERLGVMGALAVSFFALCGALRLAR
                     FNVTHVPGPFQGLPIPAGGLFLASFVLAGAHLSPWLAALLALGTGALMVSSVPYSSMK
                     KLRRGYARKKRLLLVGGAAISGAILLENAAPLAGISLYVVSGILRFDWEKWLSVPGAE
                     EERIEKSL"
     gene            complement(2166..2786)
                     /locus_tag="PK442_02390"
     CDS             complement(2166..2786)
                     /locus_tag="PK442_02390"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOO86528.1"
                     /translation="MKLAREGYPLIAFTVFMIIGSVFFAPPVALFLLPVLGLVVWFFR
                     DPERFPDGEGFLSPADGRIVEIEQADLPFTGKAWKIGIFMNPLSVHVNRFPCAGTVEH
                     LEYVPGKKWMAFAPKASEVNERMLVGISTEYGPVLLVQIAGFLARRIVCRLRKGDRLE
                     RGQRYGMIKMGSKVDVYLPAKTRLAVAKGQQVFAGRTVLGGVSCGE"
     gene            complement(2783..>4924)
                     /locus_tag="PK442_02395"
     CDS             complement(2783..>4924)
                     /locus_tag="PK442_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO86529.1"
                     /translation="NFGSVDGDSAAAMRYTEAKLFETGELMLADIDEDTVEWGPNFDE
                     SLKEPLTLPSMIPNLLVNGSSGIAVGMATNIPPHNLCEVVDALEYLIEADGNIEFGEI
                     YARLPGPDFPTGGVILGRNGIIDAYRTGRGKIILRGKTHVEEGKKGKTSIVITEIPFM
                     VNKTLLIETIASCVQEKHIDGISDIRDESDRDGLRVVLELQRDADAELVLRQLYRRTQ
                     LQSTFGVINLALVENSPKELPITDMLSLFLEHRRDVVRRRTQFRLDKALARAHIVEGL
                     VKALDIIDQVIATIRAAQTTQEAREGLVDTLGFSELQAQAILEMRLQRLTGLEREKLD
                     AELAALLADIERFRSILGNAKILDGVIKDELSLLRKKFGSPRRTEIIDSYEEVSIEDL
                     IPEEDIVVILSKDGYLRRKSLDEYVPQARGGKGRKGAALQDEDEIAVFAVTNTHKDIY
                     LFTSSGRVLAIRGHMIPESRSGKGKLVGKLVPLEDGETVVSMYGRSLEGKSYLFFATK
                     NGIAKRLQLSEILRINRGGKRVLTLDEGDEIAQVRLTSGDDELLFMTAAGQALRVPEQ
                     EFRAMGRAARGVKGIRLAKDDSVISCEVVNKERHALVISEKGVAKRTSFEEFTLHHRG
                     GSGVKAMNLGRKTGMLVGSWSVAEEDEIMAITARGRIIRVAVSEIPVLSRTAMGSITV
                     RLDGGDSVADVSVVCGEVCVAAEIPYEEETE"
BASE COUNT         1030 a         1579 c         1311 g         1004 t
ORIGIN      
        1 ctctgaatcc acacccttca cgactccctt ctctcctgcc aaaagagaga tctcgccccg
       61 gttccccttg acgattgcaa aacgaaaatc tcggagaagc gagtcgacaa gctgtctgcg
      121 aaatgaagca gcgccgtatc ccacgggatc aagcagtgta gcgcttcttt ttgcctctgc
      181 ggcggtaagc gcgttccgga taagcagttc tctatcctcc gagggagcgc ctgtattgat
      241 tagcactcct tccgcgctcc tcgcaagctc ttccgcttca tgaatacagg gcgacataac
      301 aggagaagcc ccaaccgcga ggatcgcgtt cgcttggaaa gacgcggcga tataattcgt
      361 gacggcgtaa atcaggggac ggcgagcacg gcagacactc agcgcctccg cccagtcttc
      421 ggggactatt ttcaagaccg gagccatggc ggagtcaccg catgccccag aggtccccac
      481 ccttttccgg ggcgaaaact cgcctccagc cccattcgca gatactgcct tccccggcga
      541 acagcttcct ccaaggggat accggccgca agctcggcgg taatcgctgc gctcagcgtg
      601 catcccgtgc cgtgcgtatt ttccgtgaaa atcctccggt cttcaaagag cgtgagctcc
      661 cctttataaa agaaaacatc cgttacggaa tcctcgcgga gaagatgccc gccctttacc
      721 agcacggctt tcgggcctag ctccgctatt ctcctcgacg cgcgttccat ctcctccgtg
      781 gaacggatcg aaaaaccggc cagacgttca gcctcgggaa tattcggggt cacaaggagc
      841 gcatgaggaa gcaattcctc tctgagcgct tccacggcgt catccgcgat gagcgacgcg
      901 ccgctctgtg cgaccatgac gggatcgacc acgagattcg tcacactcca ttgtcgcaca
      961 ccctgcacca ccgtccgtat gatctcgggc gagctcagca tccccgtctt tgcggcggcg
     1021 accggaaagt ccgaccagag ggcgtccatc tgcgcgcgga ccatatttgc agggacattc
     1081 catacatccg tgacggcagt gctgttctgc gctgtcaagg ctgtgatcac cgtcatgccg
     1141 aacaccttca aagcggcgca cgtcttcaga tcggcttgta ttcccgcgcc ccctcctgaa
     1201 tcgcttcccg cgatggacac cgctattccc cgatacacac tcatcacaca ttccgccttt
     1261 cctcaaataa aaaaggcccg ttcgcaatga acggaccttc tccaggctcg ctgaaaagag
     1321 ccgctcccta cgccggcatt atccggatca ggtagagggt cgaaggcatc caccttcctc
     1381 tcagcccgtt cgggctcccc cgtcactatc gattgtaaaa aaagaaaaaa gacactcaaa
     1441 ggctcttttc gatccgctcc tcctctgctc cgggaacgga aagccacttt tcccagtcaa
     1501 aacgcagtat accgctgacg acataaagcg aaattccggc aagaggcgcc gcgttctcca
     1561 gcaggattgc tccgctgatc gcggcgcccc ccaccaggag aagccgcttc tttcgcgcgt
     1621 accccctgcg cagcttcttc atgctgctgt aggggacgct cgacaccatc agcgcgcctg
     1681 ttccgagcgc aagcaacgcc gcgagccatg gagagagatg agctccggcg agaacaaaag
     1741 aggccagaaa aagcccgcct gctggaatag gcagcccctg gaagggcccg ggcacgtgtg
     1801 tgacgttgaa tcgcgcgaga cgcagggcgc cgcaaagcgc aaagaaggag acggcaagcg
     1861 cgcccatgac gccgagccgc tccgcataca ccgagtaaaa aagaacggcc ggagcaacgc
     1921 cgaagctcac aacgtctgcc aggctgtcga attcaagacc gaagcgcgtc ccgccgccga
     1981 gccgcctcgc aacctttccg tccatgaagt cgaagaaaac cgccatgaaa acgagccacg
     2041 ccgcagggac caccctgccg tggagcacaa gaatgagcga catcgtcccg cagagcagat
     2101 tgccgctcgt gaccatgttg ggcgctatct cgcggaaagg gagaggacgc cgtttctttc
     2161 tgatgctact cgccacagga cacacctccc agaaccgtac gtcctgcaaa aacctgctgc
     2221 ccctttgcaa cggcaaggcg agtcttcgca ggcaaataga catcgacttt cgaccccatc
     2281 ttgatcatcc cgtaccgctg ccctctttcg agccggtctc ccttgcggag acggcagacg
     2341 atgcggcgcg ccagaaaccc ggcgatctgc accaggagga cgggaccgta ctccgtggaa
     2401 atgccgacga gcatccgctc gttgacttcc gaagcctttg gggcgaacgc catccatttc
     2461 ttccccggga cgtactcaag atgctccaca gtaccggcgc aggggaaacg gttgacgtgc
     2521 acgctcagcg ggttcataaa aataccgatc ttccacgcct ttcctgtgaa gggaaggtcc
     2581 gcctgctcga tttccactat acgcccgtcc gcgggagaga gaaatccctc gccgtccggg
     2641 aatcgttcag ggtcgcgaaa aaaccagacg acgaggccaa ggacagggag aagaaagagc
     2701 gccacgggag gggcgaagaa aacgctcccg ataatcatga acactgtgaa tgcgataagc
     2761 gggtatcctt ctcgagccag tttcattcag tctcctcctc gtatgggatc tccgcggcaa
     2821 cgcaaacctc tccgcagaca acgctcacgt ccgctacgct gtcccccccg tcgaggcgaa
     2881 cagtgattga acccatagcc gttctgctta aaacagggat ctccgaaacg gcgacgcgga
     2941 taattctgcc tcgcgccgtg atcgccataa tttcgtcttc ttccgccaca gaccagctgc
     3001 ccacaagcat cccggtcttt cttccgaggt tcatcgcctt cacgccggat ccgcccctgt
     3061 gatggagggt aaactcctca aagctcgtcc tcttggctac ccccttttca ctgatgacaa
     3121 gcgcatgccg ctctttattc acgacttcac aacttattac ggagtcgtcc tttgcaaggc
     3181 gtatgccctt gacgccgcgc gcggccctcc ccattgcgcg gaactcttgt tcgggaacgc
     3241 ggagagcctg tccagcagcg gtcataaaga gcagttcgtc atccccggac gtcaggcgca
     3301 cctgcgcgat ttcgtcgcct tcatcaaggg taagcactcg tttccctccc ctgttaatgc
     3361 gcaaaatctc cgaaagttgc agacgtttcg ctatgccgtt tttcgtcgcg aagaagagat
     3421 agctcttccc ctccaagctc cgtccgtaca tggagacaac cgtctccccg tcctccagcg
     3481 gcacaagctt cccgacgagt ttgcccttgc cggagcgtga ttccggaatc atgtgccccc
     3541 gtatcgcgag cacacgcccg gaggatgtaa acaaataaat gtccttgtgg gtgttcgtca
     3601 ccgcaaaaac ggcgatctca tcctcgtcct ggagcgcggc gcctttccgg ccctttccgc
     3661 ccctcgcctg cgggacgtac tcgtcgaggc tcttccggcg gagatagccg tccttggaga
     3721 gaatcacgac gatatcttcc tccgggatga gatcttctat ggacacctcc tcgtagctgt
     3781 cgattatctc cgtcctcctg gggctgccga acttctttct cagaagactc agttcgtctt
     3841 tgatcacgcc gtccagaatt ttcgcattgc caagaatgct ccggaagcgt tcgatatcgg
     3901 cgagaagcgc ggccagctca gcgtcgagct tttccctttc aaggcctgtg aggcgctgca
     3961 ggcgcatttc gagtatcgcc tgcgcctgaa gctccgaaaa tccgagggtg tcgaccagcc
     4021 cttcccttgc ttcctgcgtc gtctgcgccg cccgaatggt cgcgatgacc tggtcgatga
     4081 tatcaagcgc tttgacgagc ccttcgacga tatgcgcacg ggcgagggcc ttgtcgagac
     4141 ggaactgcgt ccgtctgcgg acgacgtcgc ggcggtgttc gaggaagaga gagagcatgt
     4201 cggtgatggg gagctctttc ggactgttct ccaccagcgc gagattgatg actccgaatg
     4261 tcgactggag ctgtgtgcgg cggtaaagct ggcggagcac aagttccgcg tcggcgtcgc
     4321 gctgcagttc gagaacgacg cgaagtccgt cacggtccga ctcgtcccgg atgtcggaaa
     4381 tgccgtctat gtgcttttcc tgcacgcagg aggcgatcgt ctcgatcaac agcgttttgt
     4441 tcaccataaa ggggatttcg gtgatgacga tcgaggtctt gcctttcttc ccctcctcca
     4501 catgcgtttt gccgcggagg atgatctttc cccgcccggt gcggtacgcg tcgataatgc
     4561 cattgcgtcc gagaatcact ccgccggtcg ggaaatcagg gccgggcagc cgcgcgtaaa
     4621 tctcgccgaa ctcaatattt ccgtcggctt caatcaggta ttcgagtgca tccacaactt
     4681 cgcacaggtt atgcggggga atgttcgtcg ccatgcccac agcgatcccg gaacttccgt
     4741 tcaccaggag attcgggatc atcgagggca gggtcaacgg ctccttgagc gattcgtcga
     4801 agttcggccc ccattccaca gtgtcttcgt cgatatccgc cagcatcagc tctcctgttt
     4861 cgaagagttt ggcctcggtg tatcgcattg ccgcggcgct gtcgccgtct acgctgccga
     4921 agtt
//
LOCUS       DAOSWW010000072         4899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11626_length_4899_cov_3.819983, whole genome shotgun sequence.
ACCESSION   DAOSWW010000072 DAOSWW010000000
VERSION     DAOSWW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11626_length_4899_cov_3.819983"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PK442_02400"
     CDS             complement(<1..437)
                     /locus_tag="PK442_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO86530.1"
                     /translation="MITRMLFQLLRQIRQKVFTNPFPVAQMPDSLTDALQAAEKGWIE
                     LNPPVGVNDHFRGRLNYDKSACIGCKLCIKVCPANATEYLPEEKKIQIHVDRCCFCAQ
                     CVEICPVKCLTMSGEYLISSYDRKAQIVTDTGKPEGAPVAPAA"
     gene            complement(454..1446)
                     /locus_tag="PK442_02405"
     CDS             complement(454..1446)
                     /locus_tag="PK442_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOO86531.1"
                     /translation="MIALLMRLVAGTALMLLVVVLALLFDGADRIISARMQRRLGPPL
                     LQPFLDLLKLLGKENIVPRRAVPWVFNGAPWVAAASMLLVFLYIPVGSVPPVLGVHGD
                     LILILYLLGLSGVAMAVGGFASGNPIANVGAQREMILMMSYELPLAVVASTLAWVAYK
                     TGMPGDPFNLETFAGMSVWGMSGKIGFIGIFCLMVALILVVPGETGKGPMDIPEAKTE
                     ILDGLIIEYSGRNLAMFKITFALRAMALSALITALFFPWSPASFLRLGGIFLFTFDFV
                     WFWVKVFVVQIFVVTLMRTTFGRFKIWQASEFYLVKVAGLSIAGMILASLGLVV"
     gene            complement(1443..>1655)
                     /locus_tag="PK442_02410"
     CDS             complement(1443..>1655)
                     /locus_tag="PK442_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit"
                     /protein_id="HOO86532.1"
                     /translation="APTYANAVSWPLMFRDNELADAPLIINSIDPCISCMERMLVTDA
                     AGGRSVVTKAELLEKSREKTRRLMQK"
     assembly_gap    1656..1755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1756..2821)
                     /locus_tag="PK442_02415"
     CDS             complement(<1756..2821)
                     /locus_tag="PK442_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOO86533.1"
                     /translation="MEKKYTIPIGPVHVGLKEPITAWLDLEGEKIVGATIRPGAIHRG
                     IEFMARDRNPIQVIYLSERICGICSFAHVIAFLRAVEDAAEYPVPVRAQYIRTLVLEL
                     ERLHSHILWAGVACYSIGFDSAFHLGMVLREKVMDVLEALSGNRVNYGVATVGGVRWD
                     ITPEVAKAVREMVKYYKAEFGAFYDVVTADPIAKARMRNVGVLTPDEAVRYNAVGPTA
                     RASGIRCDIRETSPYEAYADFNVKAIVPQDYYGKAYGDVFDRFLVRVLEVQQSLNIVE
                     RVMDGLPEGEIVHEKNLNKVLASLKKAEGTGWAVLEAPRGDNTHVVHLKAGEENVNWW
                     KVRAPTYANAVSWPLMFRDNE"
     gene            complement(2834..3385)
                     /locus_tag="PK442_02420"
     CDS             complement(2834..3385)
                     /locus_tag="PK442_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOO86534.1"
                     /translation="MTFPVKGLNAETGREPEELLARIRELFGEGAVVNVRAHTAGLKE
                     EAQYRDIWLSVEKGRFLEMIDVLAEFDFPMFHVMSGNDDGDVVTLNYHFGIFQCAGRG
                     KRFGVAVSVALPKNDLVIPSLFSRIPGVEYSEREMREMLGITFDGLPNTALVFLPEDW
                     DEAVKPWRRDETGPKPENVRELS"
     gene            complement(3382..3852)
                     /locus_tag="PK442_02425"
     CDS             complement(3382..3852)
                     /locus_tag="PK442_02425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOO86535.1"
                     /translation="MNTTLLEKYLDILPKSLWVTTCNSGSCNGCDIEIVATISPRYDI
                     ERFGMKLVGTPRHADVLIVTGPVTKYMKDRLLRIYNQIPDPKAVVVVGNCGASGDVFY
                     KSYNLDGPVDNIIPVDVYVHGCPPRPEAIIEGVAKAVLKLESLKQQKKAGEADS"
     gene            complement(3865..4203)
                     /locus_tag="PK442_02430"
     CDS             complement(3865..4203)
                     /locus_tag="PK442_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOO86536.1"
                     /translation="MFGTVYTGFGYWDVTAWIVFFAIAAGLTLWLRAQGREDFKEGTD
                     QDEIYWSGNPVPEDGAEISVPASSAYWGFRKAMEPFYKVLTGMHTGIGTDIVGFYVLT
                     VAFMAVFILL"
     gene            complement(4203..>4899)
                     /locus_tag="PK442_02435"
     CDS             complement(4203..>4899)
                     /locus_tag="PK442_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOO86537.1"
                     /translation="MGLASMFFGVTMAVVQHEIKRLIGYHSVSQVGYMLLGLGVGLLV
                     LTDAHGMAEYGFTAMKGGIFHIFNYTMYKGLLFLAAGAIYYATGKRDLNEMGGLARRM
                     PFTTAMFVIAAAAISGLPPFNGFVSKLLIYESVFAVHPALTVVALLTSVLTLASFVKV
                     FQTAFLGPEKSVFTGVREVPSGMLFGMGVLTLAVLGATLFPSWTLSNLVEPAAKALVD
                     QAGYIGAVMGGGL"
BASE COUNT         1035 a         1492 c         1363 g          909 t
ORIGIN      
        1 ttcgccgcag gggcgacggg agcgccctcc ggttttcccg tatcggtgac gatctgggcc
       61 ttcctgtcat acgacgagat cagatactcg ccggacatgg tgaggcactt gacggggcag
      121 atttcgacgc actgggcgca gaagcagcat ctgtcaacgt ggatctgaat cttcttctcc
      181 tcgggcagat actccgtcgc attcgccggg cagaccttga tacagagctt gcaccctatg
      241 catgcgctct tgtcgtagtt cagacggccc cggaagtgat cgttcacacc aacgggaggg
      301 ttcagttcga tccacccttt ttcggcggcc tgcagcgcgt cggtcagcga gtcgggcatc
      361 tgcgccacgg ggaaggggtt cgtgaacacc ttctggcgaa tctgccgcag cagttgaaag
      421 agcattctgg tgatcattcc gttccacccc ttcctatacg accaggccga gggacgcgag
      481 gatcatcccc gcgatggaaa gccccgcgac cttgacgaga tagaactcgg acgcctgcca
      541 gattttgaac ctcccgaacg tcgtccgcat cagcgtcacc acaaaaatct gcaccacgaa
      601 gaccttcacc cagaaccaga cgaagtcgaa tgtaaagagg aagatgcctc ccaggcgaag
      661 gaaggatgcg ggagaccacg ggaagaagag cgccgtgatc agtgcggaga gcgccatggc
      721 gcgcagcgcg aaggtgattt tgaacatggc gaggtttctt ccggagtact cgatgataag
      781 accgtcgaga atctccgtct tcgcttcggg gatgtccatc ggccccttcc cggtctctcc
      841 gggaacgacg aggataagag cgaccatgag acaaaaaata ccgatgaagc ctatcttgcc
      901 gctcattccc cagaccgaca tcccggcaaa ggtctccagg ttgaacggat ctccgggcat
      961 tcccgtcttg taggcaaccc acgcaagcgt ggaggcaacg acggcgagcg gaagttcgta
     1021 gctcatcatc aggatcattt cacgctgcgc gcccacattc gctatcgggt ttccgctggc
     1081 gaagccgccc actgccattg caacgccgga gagccccagg agatagagaa tcaggatcaa
     1141 atctccgtgc accccgagca cgggaggcac cgagccgacc gggatgtaga ggaaaacgag
     1201 gagcatgctc gctgccgcaa cccagggcgc gccgttgaaa acccacggaa ctgccctccg
     1261 cggcacgatg ttctccttgc cgaggagctt cagcaggtcg agaaagggct gcagaagagg
     1321 cggacccagg cggcgctgca tgcgggcgct gatgattctg tccgctccgt cgaaaagaag
     1381 cgccagcacg acgacaagaa gcataagcgc tgtaccggca acgaggcgca taagaagcgc
     1441 gatcatttct gcatcagcct ccttgttttt tccctgctct tttccaggag ctccgccttg
     1501 gtgacgacgc tcctgccgcc ggcggcgtca gtgacgagca tacgctccat gcacgagatg
     1561 caggggtcga tgctgttgat aataagcggc gcgtcggcca gctcgttgtc gcggaacatc
     1621 aacggccatg aaacggcgtt cgcgtacgtc ggggcnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnngctcg ttgtcgcgga acatcaacgg ccatgaaacg gcgttcgcgt
     1801 acgtcggggc tcgcactttc caccagttca cgttctcctc gccggctttc agatggacca
     1861 catgcgtatt gtctcctctc ggcgcttcaa gaacggccca ccctgtgcct tccgctttct
     1921 tcaatgacgc gagcaccttg ttcaagttct tctcgtggac gatctcgccc tcgggaagac
     1981 cgtccatcac ccgctcgacg atattcagcg actgctgaac ctccagcacg cgtacaagga
     2041 aacggtcgaa cacgtcgccg tatgccttgc cgtagtagtc ctgcggaacg atcgccttca
     2101 cgttgaaatc ggcgtacgct tcgtacggcg acgtctcgcg gatatcgcag cggattccgc
     2161 tcgcgcgggc ggtcggcccc accgcgttgt accggacagc ctcgtccggc gtgagcacgc
     2221 cgacgttgcg catgcgcgcc ttagcgatag gatccgccgt gacgacatcg tagaacgcgc
     2281 cgaattccgc cttgtagtat ttcaccattt ccctgacggc cttcgctacc tcgggcgtga
     2341 tatcccaccg cacgccgccg actgtggcga cgccgtagtt caccctgttt ccggagagcg
     2401 cctcaagcac gtccatgact ttctcgcgga gcaccatacc aaggtggaag gcggagtcga
     2461 agcctatgga atagcaggcg acgccggccc acagaatgtg ggagtgcagc cgttcgagct
     2521 ccagaacgag cgtccggatg tactgcgcgc gcacaggcac agggtattcc gccgcatcct
     2581 cgacagcgcg gagaaacgcg atcacgtgcg cgaaggagca gatgccgcaa atcctttccg
     2641 acagatagat cacctggatg ggattccggt cgcgcgccat gaactcgata ccgcgatgaa
     2701 tcgctcccgg ccggatagtg gcgccgacaa ttttttcgcc ttcgaggtcc agccacgcag
     2761 tgatgggctc cttcaagcca acatgaacgg gacctatagg aatcgtatat ttcttctcca
     2821 tcagtcgttc ctcctaagaa agttcccgta cattttcggg cttcggtccc gtctcgtccc
     2881 tgcgccacgg cttgacggcc tcgtcccagt cctccgggag gaagacgagc gccgtgttcg
     2941 gaagcccgtc gaacgtgata ccgagcatct cacgcatctc gcgctcgctg tactcgacgc
     3001 ccggaatacg ggaaaaaagc gacggaatga caaggtcgtt cttcggaagc gccacgctca
     3061 cggccacgcc gaagcgtttg ccgcggccgg cgcactggaa gatcccgaaa tgatagttca
     3121 gcgtgaccac atcgccgtcg tcgtttccgg acatgacgtg gaacatcgga aaatcgaact
     3181 ccgcaagcac gtcgatcatt tcgagaaagc gccccttctc caccgacagc cagatgtcgc
     3241 gatactgcgc ctcttcttta agccctgcgg tatgggcgcg cacattgacg acagcgccct
     3301 cgccgaagag ttcgcgaata cgggcaagaa gttcttccgg ctcccgcccc gtttccgcgt
     3361 tgagtccttt caccggaaaa gtcatgagtc cgcctcaccc gctttcttct gttgcttcaa
     3421 tgattccagc ttcagcaccg ccttggcgac gccttcgatg atcgcctcgg ggcgcggagg
     3481 gcatccgtgg acatagacgt ccacggggat gatattgtcc acagggccgt cgaggttgta
     3541 cgacttgtag aagacgtctc ccgaagcgcc gcagttcccc accacgacga ccgccttcgg
     3601 atcgggtatc tggttgtaga tgcgcagcag cctgtccttc atatatttcg tcacagggcc
     3661 ggtcacaatg agcacatcgg cgtggcgcgg tgttcccaca agcttcatgc cgaaacgttc
     3721 gatgtcgtag cgcgggctga tagtggccac gatttcaata tcgcatccgt tgcacgaccc
     3781 ggagttacag gtcgtcaccc agagcgattt gggcagaata tccagatatt tctcaagcag
     3841 cgtagtgttc atggcgccct ccccctacag aagaatgaac acggccataa aggcgacggt
     3901 caaaacgtaa aacccgacaa tatccgtccc tattcccgtg tgcattcctg tcagcacctt
     3961 gtagaaaggc tccatggctt ttctgaaacc ccaatacgcg gaagaggcgg gaacggatat
     4021 ctccgccccg tcttcaggaa cgggattccc ggaccagtag atctcgtcct gatccgtccc
     4081 ctccttaaag tcctcgcgcc cctgggcacg gagccagagc gtcagaccgg cggcgatggc
     4141 gaagaagacg atccacgcgg tgacgtccca gtagccgaat ccagtgtaga cagtgccgaa
     4201 catcagagcc cacctcccat aacagcgcct atatacccgg cctgatcgac gagagccttc
     4261 gccgcgggct cgacgagatt cgacaaggtc cacgacggga agagcgtagc gccaagaacc
     4321 gcgagcgtca gcactcccat gccgaacagc attcccgacg gaacctccct gacgcctgtg
     4381 aagacgctct tttcgggacc gaggaaggcc gtctgaaaga cttttacaaa ggaagccagc
     4441 gtcagcaccg acgtgagcag cgctacaact gtcagcgccg gatgaacggc gaagactgac
     4501 tcatagataa gcagcttcga tacgaaaccg ttgaacggag gaagaccgga gatcgccgcc
     4561 gccgcgatca caaacatggc ggtggtaaag ggcatgcggc gcgcgagacc gcccatttca
     4621 ttcaagtcgc gcttccctgt cgcgtagtag atggcgccgg cggcaaggaa gagcagcccc
     4681 ttgtacatcg tgtagttgaa gatgtggaag atgccgccct tcatcgcggt gaagccgtac
     4741 tcggccatcc cgtgcgcgtc ggtgaggacg agcagaccca cgccgagtcc gagcagcatg
     4801 tacccgacct gcgaaaccga atggtagcct atcaggcgct tgatctcgtg ctgcaccacg
     4861 gccatggtca ccccgaagaa catggaagcg agtcccatc
//
LOCUS       DAOSWW010000073         4897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11632_length_4897_cov_4.480380, whole genome shotgun sequence.
ACCESSION   DAOSWW010000073 DAOSWW010000000
VERSION     DAOSWW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11632_length_4897_cov_4.480380"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PK442_02440"
     CDS             <1..814
                     /locus_tag="PK442_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86538.1"
                     /translation="KKLKVLHNLSFVEDPTRVIRGVRLEQRLGLTMEDNTLRLIRSCI
                     RGGLLVRLSGFRLRSELELSFREKFPWAAARRMGELGVWDVLFPGIRIDESVRRTFRR
                     LGAFIARISRDFPDFKGRQWLAFFSALLMESSENIRISALDRLNLSESERGIVVKCLS
                     GLGAAEHTLGGRSSPLNSEIAAFLDGHDPLEAFFWSAATERWRVRRRILQYLTRLHRV
                     RPILSGGDLLQLGYAATPRIGVILEKLRILRLDSVVQTREEEEEYVRKHFPL"
     gene            938..1537
                     /locus_tag="PK442_02445"
     CDS             938..1537
                     /locus_tag="PK442_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO86539.1"
                     /translation="MPGIADLLLSVPAVLWAITFHEFCHGYMAYRLGDPTAERAGRLT
                     LNPLRHLDPVGALMLLVFRFGWAKPVPVDPRYFKNPRRDMLLVSIAGVSGNFFTAFVF
                     GMLARFFPYVFLANAALRQFVVLMILINLGLAVFNLIPIPPLDGSKLLYPLLPSFMLE
                     KYFWLERYGFFVLMILMALGVVQAIMGPIVMFFFRLFLF"
     gene            1553..2338
                     /gene="surE"
                     /locus_tag="PK442_02450"
     CDS             1553..2338
                     /gene="surE"
                     /locus_tag="PK442_02450"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOO86540.1"
                     /translation="MKLLITNDDGVFAPGIITLASFLAERGEECTVVAPDRERSSVGH
                     AITLTRPLRLWAIPSGLYSSTCSVYACDGTPSDSVMLGLEEIIPEPDLVLSGINKGPN
                     LGDDLTYSGTVSAAMEALILGRPSLAFSLNCRDADEEQHYETAAAVAGSLLDFLRGSP
                     LPEGVLLNVNVPNVPLPLLKGIKVTRKGVRLYEGKVTRLKDPAGRSYFWVSGRPEDRL
                     EEGSDVWAVAGGFVSVTPVHMDMTHFPSLQTLTANGAEKINFK"
     gene            2654..3880
                     /locus_tag="PK442_02455"
     CDS             2654..3880
                     /locus_tag="PK442_02455"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOO86541.1"
                     /translation="MTKKGKVVLAYSGGLDTSVAVVWLKEQGYDVITMTADVGQQAVD
                     LEAAKSKALRTGAEKAYVLDLKKQFVEKFVWPSLKANGMYQSVYPLNSALSRPLIAEV
                     MAEIAKKEGAVAVAHGCTGKGQDQVRIEVCTNALNPDLAVLAPVRDWHFSREAEMEYA
                     QAHGIPVQATHASPYSIDDNIWGRSIECGILEDPWNEPPSDAYTLTCEPHEAPDQPEY
                     VEILFEKGIPVALDGVRMDGPTLIDKLNCQAGKHGVGRIDMLEDRLVGFKSREVYECP
                     AAITLITAHRALETLTLDKRVLATKKELEVKFGELAYEGYWFSPLKDAINSFIDTTQE
                     YVNGVVKVRLYKGMAVVRGMKSGKSIYSETLATYSENDAFDHSAAVGFIKIWGLPIKT
                     WRQVHESKEAIEIPAQ"
     gene            complement(3931..4452)
                     /gene="purE"
                     /locus_tag="PK442_02460"
     CDS             complement(3931..4452)
                     /gene="purE"
                     /locus_tag="PK442_02460"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOO86542.1"
                     /translation="MVSPEVGILIGSKSDYPLVKKVEDVFAFFGVSWEISVASAHRTP
                     DDVVAYVNNAPKRGIKVLVAAAGLSAALPGVIAAHTRLPVIGIPVNAGTVGGLDALLS
                     VAQMPPGVPVASVGIDGVKNAALFAVRILALVREDLAGKLDAWSKKEEDAVRTARNDL
                     EGAHSAPGEAFTL"
     gene            complement(4460..>4897)
                     /locus_tag="PK442_02465"
     CDS             complement(4460..>4897)
                     /locus_tag="PK442_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HOO86543.1"
                     /translation="NVRFGDPEAQVVLPLLEVDFAEMLFACCEGRLSEFRWEEPSRWA
                     VDVVLASGGYPGTFEKGKKIEGLENVSSMEDFVLFHGGTTFSPEGKILTEGGRVLSAV
                     GLGASLEEALVNAYAGVEQISFENMHYRRDIARRAIIQRRGGI"
BASE COUNT         1104 a         1311 c         1357 g         1125 t
ORIGIN      
        1 aaaaaaactg aaggtactcc ataacctgag cttcgttgaa gatccgacgc gagttatccg
       61 gggcgtgcgg ctggaacagc gcctcggact gaccatggag gacaatacgc ttcggctgat
      121 acgaagctgc atccgaggcg gacttctggt ccgtctttca gggtttcgcc tccggagcga
      181 gctggagctc tcattccggg aaaagtttcc ctgggctgct gcgcggagaa tgggggagct
      241 gggggtttgg gacgttctgt ttccgggtat tcgaattgac gagagcgtcc gcaggacttt
      301 ccgcaggctc ggcgccttca ttgccagaat atccagggat ttcccggatt tcaagggacg
      361 ccaatggctt gcgttttttt ccgcactcct tatggagtcc tccgaaaata tacgtatttc
      421 ggcgctggat cggttgaatc tctctgaatc ggaacgcgga attgtagtaa aatgcctttc
      481 ggggttgggc gccgctgagc acacgctggg aggtaggagc agcccgctca actccgagat
      541 tgccgcattt cttgacgggc acgatccatt agaagcgttc ttttggagcg cagcgactga
      601 gcgctggcga gtccgcagga gaatccttca gtaccttacg cgccttcaca gagtccgccc
      661 gattctttca ggaggtgatc ttctgcagtt ggggtatgcc gcaacccccc gcatcggcgt
      721 catcctcgaa aaattgcgga tcctgcgcct ggacagcgta gtgcaaacca gggaagaaga
      781 ggaagagtac gtcagaaaac acttcccgct gtaacgcgca gcacgcgggc cccctcttcc
      841 ctacggtttg ccgatgagac ggaggagcac gttctgaacg gcgaggtgcg gcaaaaaacg
      901 ttacaacaag ttaaggagag tgactctatt gttacgaatg cctggtattg cagatctgct
      961 tctcagtgtt ccagctgtcc tttgggcaat tacatttcat gagttctgcc atgggtatat
     1021 ggcgtaccga ctcggcgatc cgactgcgga gagggcggga cgtttgacgc tgaatccgct
     1081 ccgtcatctt gacccggtag gagcgttaat gttgcttgtc ttccggttcg gatgggcgaa
     1141 gccggttcca gtagatccga ggtattttaa aaatcccagg agagacatgc ttctggtctc
     1201 catcgccggg gtttccggaa acttttttac agccttcgtt tttggcatgc tcgcgcgctt
     1261 tttcccgtat gtttttctgg caaacgctgc gctcaggcaa tttgtggttc tgatgatttt
     1321 gataaacctc ggcttggcgg tgttcaacct catcccgatt cctcctctcg acggctcgaa
     1381 gctgctttat ccgttgctgc cctccttcat gctcgaaaaa tacttctggc tggagcgtta
     1441 cggcttcttt gtcctgatga tacttatggc tctcggagtt gttcaggcaa ttatgggacc
     1501 gatcgtgatg tttttcttcc gtctctttct tttctaggag aagtcgaatc gcatgaaact
     1561 tcttataacg aacgacgacg gcgtttttgc acccggaata atcaccctcg cctcttttct
     1621 ggcggaacgg ggtgaagagt gcactgtggt agctccggac agggaacgaa gcagcgtcgg
     1681 ccacgctatt actctcactc gccctctccg cttatgggca atcccatcgg ggctctattc
     1741 ctctacgtgt tcagtgtatg cgtgtgatgg gaccccctcc gacagcgtca tgctgggatt
     1801 ggaggaaatc attccagaac cggatctcgt gctttccggc atcaataaag gcccaaacct
     1861 gggcgatgat ctcacctatt ccggcactgt ttccgcggcg atggaggcgc tgattcttgg
     1921 acgcccttcg cttgcattct ctctgaattg cagggacgca gacgaggagc aacattacga
     1981 aacagccgcc gctgtcgccg gatcgcttct cgattttttg cggggttctc cgctccccga
     2041 aggggtgctg ctcaacgtaa acgtccctaa tgtaccgctt cctctgttga aggggataaa
     2101 agtgacgcgg aagggagtgc gtctttacga gggcaaggta acgcgcctga aggaccccgc
     2161 agggcgctcc tatttctggg tctccggaag accggaggac aggctggaag aggggagcga
     2221 cgtttgggcg gttgccggcg gattcgtctc agtaaccccg gtgcatatgg acatgacgca
     2281 cttcccctct ttacagacgc tgacggcgaa tggagcagaa aaaataaact tcaagtgagt
     2341 gatttcaact tacttgacaa atgtttcgca gtcggtataa tatccctact caagcgtatt
     2401 ggcgatgatg aggaagagta ctccgcagca cagcaacaga gaggacgggc cacggctgag
     2461 agcccttccg gcaggtcacg gaggaagaga gccttggagc cggtttcgaa gaaaggcctc
     2521 ggccaagtag aaacggacag agagggcgga aattccgtca aaaagggtgg taccgcgggt
     2581 tcaagactcg tccctggtaa attggggcga gtctttattt atttagttcg actattcttt
     2641 tggagggata aaaatgacaa aaaaagggaa agttgtcctt gcatacagcg gcggtttgga
     2701 tacttcagtg gcggtggtat ggctcaagga gcaggggtat gacgttatca cgatgacggc
     2761 cgatgtaggg cagcaagccg ttgatcttga ggccgcgaag agcaaggctc ttcgtactgg
     2821 cgccgaaaaa gcctatgttc ttgacctgaa gaaacagttt gtggagaaat tcgtatggcc
     2881 ttcattgaag gccaacggga tgtatcagag cgtctatccg ttgaactccg cgctctcgcg
     2941 cccgcttatt gccgaggtta tggcggagat cgcaaaaaaa gaaggcgccg tcgcggtcgc
     3001 ccacggctgc acaggcaaag gacaggatca ggtgcgcatc gaagtgtgca ccaacgcgct
     3061 taatcctgat ctcgcggtgc ttgcccctgt cagggactgg cacttctccc gcgaagccga
     3121 gatggagtat gcgcaagctc acggcatccc cgttcaggcg acccatgcct ccccgtacag
     3181 catcgacgac aatatctggg ggcgctccat cgaatgcgga attttggagg atccgtggaa
     3241 cgaacctcct tccgacgcct acacactgac ctgcgagcct catgaagccc cggatcagcc
     3301 tgaatatgtg gagattctgt tcgagaaggg aattccggtc gctctggacg gcgttcgtat
     3361 ggacggcccg acgcttattg ataaactgaa ctgtcaggcg ggaaaacatg gagtagggcg
     3421 gatcgacatg ctcgaagaca gacttgtcgg tttcaagagc cgcgaggttt atgagtgtcc
     3481 ggcggctatc acactgatta ccgcgcatcg cgcccttgag actctcactc ttgacaaacg
     3541 cgttcttgca acgaagaaag aactcgaagt caaattcggc gaacttgcct acgaagggta
     3601 ttggttctcc ccgttaaagg acgcgatcaa ctccttcatc gacaccacgc aggagtatgt
     3661 caacggcgtg gtcaaagtcc gtctgtataa gggaatggcc gtggttcgcg gaatgaaatc
     3721 cggtaagtct atttacagcg agactcttgc cacttactcg gaaaacgatg cattcgacca
     3781 ctctgcggca gtcggcttca tcaagatctg gggcctgccg attaaaacgt ggaggcaagt
     3841 ccacgagagc aaggaagcca tcgaaatccc tgcacagtaa aaggacaaga aaaaacggag
     3901 gaaccgagga tatcccggtt cctccgtttt ttacagagta aacgcctcgc cgggagcgct
     3961 atgcgccccc tcaagatcgt ttcgcgccgt tcggacggcg tcttcctctt tttttgacca
     4021 cgcatcaagc ttgcccgcaa gatcctctcg tacaagcgcg aggatacgta ccgcgaaaag
     4081 cgccgcattc ttcaccccgt ctatgcccac tgaagccacc ggcacgccgg ggggcatctg
     4141 cgctaccgag agaagcgcgt caagtcctcc gacagtcccg gcgttcaccg gaataccgat
     4201 gacaggaagg cgcgtgtgcg ctgcaataac gcccggaagg gcggcagaga gtcctgcggc
     4261 ggccacgagg actttgattc cgcgtttcgg cgcgttgttc acgtaggcga cgacgtcatc
     4321 cggtgttctg tgagcggatg caacggaaat ctcccaagaa actccgaaaa aagcaaagac
     4381 atcctcgact ttcttgacca agggataatc cgattttgat ccgatcagta tcccaacttc
     4441 tggtgaaacc aacggcatat cagatgcctc ctcttctctg aataatcgcc cttcgggcaa
     4501 tgtctctcct gtagtgcata ttttcaaagg aaatttgttc aacacccgca taggcgttta
     4561 cgagagcttc ctccagcgat gccccgaggc cgacggcgct gaggacccgt ccgccctctg
     4621 tgagaatctt tccctcggga gagaaggttg tgcctccgtg gaaaagaaca aaatcttcca
     4681 tggatgaaac attctctagt ccctctatct ttttcccttt ctcaaacgtc ccggggtatc
     4741 ccccggaggc aagcaccaca tccaccgccc atctggacgg ctcttcccac cggaactctg
     4801 aaagacggcc ttcacagcat gcaaaaagca tttccgcaaa atccacctcc aaaagaggga
     4861 gaacgacctg tgcttcggga tctccaaaac gcacgtt
//
LOCUS       DAOSWW010000074         4897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11634_length_4897_cov_3.771375, whole genome shotgun sequence.
ACCESSION   DAOSWW010000074 DAOSWW010000000
VERSION     DAOSWW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11634_length_4897_cov_3.771375"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(414..1820)
                     /locus_tag="PK442_02470"
     CDS             complement(414..1820)
                     /locus_tag="PK442_02470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO86544.1"
                     /translation="MLSRIERVDVMKEHAEILIVEDSLTQAKRLERLLTQNGYVVYVA
                     RNGAEGLEAAARKKPTVVISDVMMPEMDGYEMCRRIKNTEEIKGTPVVLLTSLSDPRD
                     VIKGLECGADNFLTKPYDGEHLIRRLEHILTNLELRKAGQAQMTVEVFFAGQYHRLTA
                     DRIQIIDLLLSTFEAAVLQSTQLEKLGGDYRNALEDVKRAQANFQTLMETTGDAVIVA
                     GDNGTIYYANPAAENLFDTKASDLVGRPFAFPLDVKGEEHREITISRSDGSKVVGDMR
                     VATSNWDGELVQFATIRDISEMVHLRELLKTEAITDALTGLHNRRGFMLLGEELLRKA
                     LEKESNVLCFFADLDGFKSVNDTLGHEEGDKVLQESAEALRKTFRSSDILARVGGDEF
                     AALVLHDAAGNETDIIGRLRQALDTINSRSLRSYRIVMSAGISLWEPENPISLGELMS
                     DADKKMYREKEERKKVSK"
     gene            complement(1913..2974)
                     /gene="cheB"
                     /locus_tag="PK442_02475"
     CDS             complement(1913..2974)
                     /gene="cheB"
                     /locus_tag="PK442_02475"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOO86545.1"
                     /translation="MADVLIVDDSALLRRILKQMLSKDPGLNVIGCVESGEAALQFLE
                     NTRVDVVTMDIHMPGMGGFDATRRIMESPHPVPVVIISSCWDPNEVEKTFKAMDAGAV
                     AILPKPADISSGDDEYSAELLKTINEAAVSKVGRLRQRKRPMKSDDAPRNPLQPSPSH
                     ANGRSERRISLVGVGASTGGPQALAEFLARLPADFPRPLLVVQHIAAGFTSGLAEWLG
                     KVTPLPVRVAQNGQKAMNGVVHIAPDGFHMGVRQGGFLSLAKDPPEHMVRPSASVLFR
                     SLAAVYGGAAAVILFSGMGKDGAAEMKTLRNMGAATFAQDRESSVVWGMPGEAVRLDA
                     AEHVGSPAAIASLLLDQFR"
     gene            complement(3001..>4897)
                     /locus_tag="PK442_02480"
     CDS             complement(3001..>4897)
                     /locus_tag="PK442_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86546.1"
                     /translation="GSRRPHAEEDKPEGKDEALSLPDRALPSHAVSPAVTEASPQPKE
                     ERSRHTGQETVRIAASKLDSLLLKAEELISVNLALEVRIENIREILSLSAEFKSEWEK
                     TLDALRREEQAIEKLPQKLQGFLQSGRDSFRKILETLRDMEKALRSDQRAASGLVRDL
                     LEGARTVLMLPFSSLLQSFPKIVRDLARELGKEANVNIRGGEVEVDKRILEGLKDPLV
                     HLVRNCVDHGLEKAEDRRAAGKPPEGTITLTVSQTDGGRVEILVADDGKGINAEKLRE
                     IAVKNGLISREEAALLDDAAAMSLIFRSGVSTSPLITDISGRGLGMAIVQEGVEALGG
                     SIVLESKPGKGSSFRLSLPLTLATFRGVLVEEWGRVFVIPSSGVERVARVEKSKIRLI
                     EQRESVSFNGTPLALARLGHILELPSPPERLKIRETLPLVVIGAGGVSVAFGVDKVLN
                     EQDILLKPLGKQLERVRNVSGATVLGSGMVVPVLNTADLIRSVQRSGGGRGFLSDPEE
                     KKTRRNVLIAEDSITSRTLLRNILSAAGFVVQTAVDGQEAWDYLAKGGFHIVVSDVEM
                     PRMNGFELTAKIRSDPATADIPVVLVTSLESREDRERGAEAGADAYIVKSGFDQGNLL
                     DVLRRLL"
BASE COUNT         1029 a         1536 c         1222 g         1110 t
ORIGIN      
        1 ccaccgtgtc ccaacccccg aagaggaaat gccccttttc attgatgtga tcactgttgc
       61 ccaaagtacg cgcacctctt tcttcattcc cggcgaaata tgaaaaatgg cccgtcccaa
      121 tgaagggacg agccatatgt ctcgcggtac caccctgctt ggacgcattt ttgcgtcccg
      181 ctctcggctc tataacggga gcctcccgtg ggcgtacttc cggtcgtgac cgtttcagcc
      241 cccgctccgc agtctttgcg cctttcgccc ttgccgaaga gaccttccag ccttcggatc
      301 tctctctctg gcggcaaccg tgaacgcagg caatctgcat catcgcgtgt acatcttatt
      361 tattcgtgac attatagcca gtacatacgg aaacgtcaac agtaaaaaaa ctgctacttt
      421 gaaacttttt ttctctcctc tttttcacga tacatcttct tgtccgcatc cgacatcagc
      481 tcgccgagag aaatggggtt ttccggctcc caaagcgata ttcccgcgct catcacgata
      541 cggtaggatc gcaggctccg cgaattgatc gtatcaagcg cctgccgcaa cctcccgatg
      601 atatccgttt cgtttcccgc agcatcgtga agcacgagag ccgcgaattc atccccgccg
      661 acccttgcca gtatatccga agaacggaag gtctttctga gcgcttccgc cgactcctgg
      721 aggactttgt ccccctcctc gtgaccgagc gtgtcgttca ccgacttgaa cccgtcgaga
      781 tcggcgaaga agcagaggac gttcgactcc ttttcaagag ccttccgcag aagctcttct
      841 ccgaggagca tgaaccctct ccggttgtgg agccccgtaa gcgcgtcggt aatcgcctcg
      901 gtcttcaaga gctccctgag atggaccatc tcggaaatat cacgaatggt agcaaattga
      961 acaagctctc cgtcccagtt ggaggtcgcc acgcgcatat cgcccacgac cttcgacccg
     1021 tccgaccgtg aaattgtgat ctccctgtgt tcttccccct tgacgtcgag cgggaaggca
     1081 aaaggacgtc ctaccaggtc ggacgccttg gtgtcaaaaa gattctcggc ggcggggtta
     1141 gcgtagtaga tagtgccgtt atcgcccgcc acaatcacgg cgtcgccggt agtttccatc
     1201 agcgtctgaa agttggcctg ggcccgtttc acgtcttcga gggcattccg gtagtccccg
     1261 ccgagctttt caagctgcgt gctctgcagc acggccgcct cgaatgtgga gagcaacagg
     1321 tcgatgatct gtatccggtc cgcagtgagc cgatgatact gccctgcgaa aaaaacctcg
     1381 accgtcatct gcgcctgccc ggcttttctc agctccaaat tggtcagtat atgttcgaga
     1441 cgccggatca gatgctcgcc gtcgtacggt ttggtaagaa aattgtcagc gccgcattca
     1501 agccctttga tcacgtccct cggatcggag agcgaggtga gcagtaccac cggcgtcccc
     1561 ttgatttcct cggtgttctt tatgcgcctg cacatctcgt acccgtccat ctcgggcatc
     1621 atgacgtccg agatcaccac cgtgggcttt ttgcgggcgg cggcttccaa cccctcggcg
     1681 ccgtttctgg ctacgtacac aacgtagccg ttctgcgtaa ggagacgctc caaccgcttc
     1741 gcctgggtca gactgtcctc gacaatgagg atctcagcat gttctttcat cacgtcaacc
     1801 ctttcaatcc tgctcaacat ggagtggtcc ctccatattt tcggtatatt ttccccctgt
     1861 atgaagtgcg tttcactctc gccaaagaat catgcgcgcc accggtctca gattaacgga
     1921 actgatccag aagaagggag gcgatcgccg cgggagatcc tacatgttcc gctgcatcca
     1981 ggcgcacagc ctccccgggc ataccccaga caacggaact ctctctgtcc tgggcgaacg
     2041 tggcggcccc catattccga agcgttttca tttccgcggc gccgtccttt cccattccgg
     2101 agaagaggat gacggccgcg gctcccccat acactgcggc gagactccgg aagagcactg
     2161 acgcggacgg acgaaccata tgctccggcg gatcttttgc aagagaaaga aaacctccct
     2221 ggcgaactcc catatgaaaa ccgtcgggag caatatggac gacgccgttc atcgccttct
     2281 gtccattttg ggcgacacgt accggaagag gcgtcacttt tccaagccac tccgccaatc
     2341 ccgaggtaaa cccggcggct atatgctgca cgacgaggag agggcgcggg aaatccgccg
     2401 gaagccgcgc aagaaactcc gcaagagcct gcggacctcc tgtcgaggcg ccgacgccga
     2461 caagcgatat ccgtcgttca gatcgcccgt ttgcatgaga cggagagggc tggagaggat
     2521 tccgtggagc atcatcgctc ttcatcggcc tttttctctg ccgcagacgt cctacttttg
     2581 atacggcagc ctcgtttata gttttcagaa gctccgcaga gtattcgtca tcgcctgagg
     2641 agatatccgc gggcttgggg agaatagcca cggcgcctgc atccatagcc ttgaacgtct
     2701 tttcgacttc gttcggatcc cagcatgaac tgataatcac cacaggcaca ggatgcggag
     2761 attccatgat ccggcgtgtt gcgtcgaaac cgcccatgcc cggcatgtga atatccatag
     2821 tcacaacgtc gaccctggta ttttcgagga actgcaacgc cgcttcgccg ctctcgacac
     2881 acccgataac gttcagcccc ggatccttac tcagcatctg cttcaagatc ctgcgcagca
     2941 acgcggaatc atcaacaatg agaacatccg ccatgccgaa cgccccctct cttttacact
     3001 ctacagaaga cgacgcaata catcaagaag gtttccctgg tcgaaaccgc ttttcacgat
     3061 atatgcgtcg gctccggcct ctgcgccccg ttccctgtcc tctctggatt caagagatgt
     3121 caccagcacc actgggatat cagccgtcgc agggtcggag cggattttcg ccgtcagctc
     3181 gaacccgttc atccggggca tttcgacgtc ggagaccacg atatgaaacc cgcccttcgc
     3241 aaggtaatcc cacgcctcct gtccatccac ggctgtctgc acaacaaaac ctgcggctga
     3301 gagaatattc cgcaggagag tacgcgaagt aatggaatcc tcggctatga gaacgtttct
     3361 gcgggttttt ttctcttccg ggtcggaaag aaaacctcgt cccccgccgc tcctctgaac
     3421 agaacggata agatcggccg tgttgagcac gggaacgacc attcccgatc cgagcacggt
     3481 ggcgcccgag acgttccgca cccgctccag ctgtttccca agcggtttga gcagaatatc
     3541 ctgttcattg agcactttgt cgacgccaaa cgccacggag acccctccgg ctccaatcac
     3601 aacgagaggt aacgtttccc gaatcttaag ccgttcaggg ggagacggca gctcaagtat
     3661 atgtcccagt ctcgccagcg ccagcggcgt cccgttaaac gacacgctct ctctttgttc
     3721 tataagcctg attttgcttt tctccaccct ggccacgcgc tccacaccag aggatggaat
     3781 aacgaagaca cgcccccact cctccaccag cactccgcgg aacgttgcaa gcgttaacgg
     3841 aagagaaagg cgaaaagaag acccctttcc gggctttgat tcaaggacga tgctacctcc
     3901 aagggcttcg acgccttctt gaacaatcgc cattcctaac cctcgccctg aaatatctgt
     3961 aataagcgga ctcgtggaaa cgcctgacct gaagatgagc gacattgcag cggcatcgtc
     4021 gaggagcgcg gcctcctccc ttgagatcag gccgtttttg acggcgattt cgcgaagttt
     4081 ttctgcattg atcccttttc cgtcgtccgc aacaagaatt tccaccctgc ccccgtcagt
     4141 ctgagagacc gtaagcgtga tcgtcccctc gggaggtttt cctgcggcgc ggcgatcttc
     4201 cgccttttcc agtccgtggt cgacgcagtt gcgcaccaga tgaaccagag gatccttcaa
     4261 tccttcaaga atccgtttgt caacttcgac ttccccgccc cgtatgttca cgttcgcttc
     4321 tttaccaagc tcgcgcgcaa gatcgcggac tattttcggg aagctctgca agagcgacga
     4381 aaacggaagc atcagcacag tcctggctcc ctcgaggaga tcgcggacaa gcccgctcgc
     4441 ggcccgctga tcggaacgca gcgctttttc catgtcccgc agcgtttcga gaatttttcg
     4501 aaaagaatct cttcccgact gtaaaaaacc ctgcaatttc tggggaagct tttcaatggc
     4561 ctgctcttcc ctccggagcg cgtcaagagt cttttcccac tcgcttttga actctgcgga
     4621 aagcgacagt atttcccgta tattctctat gcggacttcc agagcaagat tcaccgaaat
     4681 cagttcttcg gccttcagca gcaacgaatc cagtttcgag gcggcgatac gcacggtctc
     4741 ctgcccggta tgtcgtgagc gctcttcttt cggctgcggg gaggcttccg ttacagccgg
     4801 agagactgca tgagacggaa gagcgcgatc cggaagcgag agcgcttcat cttttccctc
     4861 cggcttatct tcctccgcgt gcggtctccg gctcccc
//
LOCUS       DAOSWW010000075         4876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11696_length_4876_cov_2.981539, whole genome shotgun sequence.
ACCESSION   DAOSWW010000075 DAOSWW010000000
VERSION     DAOSWW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11696_length_4876_cov_2.981539"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1514
                     /locus_tag="PK442_02485"
     CDS             240..1514
                     /locus_tag="PK442_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86547.1"
                     /translation="MKIIARRKRERTTESAEARNEEMVLSSEAEHRAEGGLSPEESSS
                     ETSSDATGCSVPSTPSEEKETIDRDSMWKTIMVGNFDHMLDSILPELAAKVDRSRRPE
                     FLDKEMKDLKALMGGTWQEPDLLAKVPVKEGRDIWLALHVEVQDKGGEDFPERMFYYH
                     SMIRFKYLKRKRYEEEQAAKASVQSDGKRKMAKERRGVVDVVSMAILTARRPKGEAEF
                     YEHASYGNEMRYIYPAVRLWELDSEQLVASFNPFDLALLAAKRMIESGRSDNKRIIFL
                     KHLGSLLDERGWSRERSVALYRFIEWILRPKSEKKYEEYKAWVRKEERKVYITAAEEK
                     GMEKGLEKGVEIGKEIGKEIGKEIGKEIGKEIGKEIGKEIGKGEGILIGKEDTKREAA
                     LRMLRKGMEVSLVAECVDLPEDEVRRLCEERS"
     gene            2078..3061
                     /locus_tag="PK442_02490"
     CDS             2078..3061
                     /locus_tag="PK442_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86548.1"
                     /translation="MNTCSEVFIITHAGRSIGGGHASRCSALAEGFSSLGVAVRWLVN
                     ASAGEIVLRRGATEASVTVMESPFGGGADAVFTAISRSRPALCVVDGYDATPSFLREL
                     RRFAPVVLVDDCRVRPVEYECDVVLNYNVNADLLGYEKGHAGLLLGPEYALLRSEFWS
                     LAPEKGDAVLIIPGASDLLHTGERFVEWWGSDWPRAELVLGPLVESSMAERLSEAAGA
                     LPNLSAVRNPLDLPARMARSRAVLCTSSVTSYEALALRKPLVVFQTAENQAGIGCEIE
                     RRGLGVNLGAWGTWGGERLRRSLEDLPPVPPACVNPRGACAAAEAVLCAVR"
     gene            3287..3484
                     /locus_tag="PK442_02495"
     CDS             3287..3484
                     /locus_tag="PK442_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86549.1"
                     /translation="MTMNFFDAVKNRRTYYSISKKSPVSDERIREIVEQAVKHCPSAF
                     NSQSGVPCCFSGRRATRSGVL"
     gene            3397..3885
                     /locus_tag="PK442_02500"
                     /pseudo
     CDS             3397..3885
                     /locus_tag="PK442_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            4139..>4876
                     /locus_tag="PK442_02505"
     CDS             4139..>4876
                     /locus_tag="PK442_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOO86550.1"
                     /translation="METQSGKGPHFIEQIIMDDLKNGVVKEVHTRFPPEPNGYLHIGH
                     AKSICLNFGLAEQFGGKCNLRFDDTNPAKEETEYVESIMRDVQWLGFRWHTLRFASDY
                     FQQFYEWALELIHAGKAYVDHQTPDEIRETRGTLTAPGVESPYRNRSVGENLALFQQM
                     KDGVLEDGTCVLRAKIDMNHPNINMRDPVLYRILHRHHFRTGDAWCIYPMYDFAHGYE
                     DAIEGVTHSICTLEFEDHRPLYEWFLDN"
BASE COUNT         1039 a         1255 c         1565 g         1017 t
ORIGIN      
        1 tgtaatggtc tgttcctcgt gcacgtcgtt gctcactgcg tcgcctcctt tgcaggtata
       61 atgatagtac atcatgatgc gtgcatactg ggtggtttat ggaaagagtg aaggtaacta
      121 gccaggcgct gtgattgcag aagtccgcct cttttcgtat aatattcgtg aaacacaaaa
      181 cgcacgctat cgagaagtag cggaagaccc cctgcgaggg gcatcattcg cgaggaggct
      241 tgaagattat cgcacggcgg aagagagaac gaacgaccga gagcgccgaa gctcgcaatg
      301 aagagatggt tctctcttcg gaggccgagc accgggcgga gggtggcttg agccctgaag
      361 aatcctcttc ggaaacatct tcggacgcga cgggctgtag cgtaccctcg acgccctcgg
      421 aggagaaaga gacgatcgac cgtgattcca tgtggaagac gattatggtg gggaacttcg
      481 accacatgct cgactccatt ctccccgaac tggccgcgaa ggtcgaccgc tcgcgccggc
      541 cggagttcct cgacaaagag atgaaagatc ttaaggcgct catgggcggc acgtggcagg
      601 agcccgacct tctcgccaag gtcccggtga aggaagggcg cgacatctgg ctcgctctcc
      661 acgtcgaagt gcaggacaag ggcggggagg acttccccga acgaatgttt tactatcatt
      721 ccatgatccg cttcaagtat ctgaagcgga agagatacga ggaggagcag gcggccaagg
      781 cctccgtgca gagcgacggg aagcggaaga tggcgaagga gcgccgtgga gtcgtggacg
      841 tggtctcgat ggctatcctc acggcgcgca ggccgaaggg agaggccgag ttctacgagc
      901 acgcctccta cggcaacgag atgcgttaca tctatccggc ggtcaggctc tgggaacttg
      961 actccgaaca gctcgtagca agtttcaatc ctttcgatct cgcgcttctt gcggcgaaga
     1021 ggatgatcga aagcggccgc tcggacaaca aacggatcat ttttttgaag catctcggaa
     1081 gtcttctgga cgagcgcggg tggtctcgcg aacgcagcgt cgcgctctac cgcttcatag
     1141 aatggatact tcggccgaag agtgagaaga agtacgaaga gtacaaggca tgggtaagga
     1201 aggaggagag gaaagtgtac atcactgcag ccgaggagaa aggcatggag aagggattgg
     1261 aaaagggcgt ggagatcgga aaggagatag ggaaagagat aggaaaagag ataggaaagg
     1321 agataggaaa ggagatagga aaagagatag gaaaagagat aggaaagggc gaaggcatcc
     1381 tcatcgggaa ggaggatacc aagcgtgaag ccgctctcag gatgcttcgc aaagggatgg
     1441 aggtttccct cgttgcggag tgcgtcgacc tcccggaaga cgaagtccgc cgcctttgcg
     1501 aagagaggag ctgagcacga ccgtcgcgtg cggagaatgt gtctggaagc cgtgcttttg
     1561 attacgtgac ggcataacgc cgaattttta agacgtgcct ttcgcgtgtc attttaagcc
     1621 ttgctgtatt ccctggcgag aagccgtcga gtgttataat caagcagagc cacttgctaa
     1681 caaagaggtc tttggattgt ctgttgatgt caaggagcaa aaaactgtat cgattggtga
     1741 gccgtcttcg ccgggaggat gtggagagag tgacgagggg tgagggtgcc gctcagtttt
     1801 tgcgcgattc gaccatctcg tcgagttcct ccggagatat ctccctatga ccagaatctt
     1861 ttcgtgaggg ttacctttgc acagcatctt agcgacaacg gtaaattttt gcttcttctc
     1921 gtctctcttc tcacctaacg tctcccctct ctcctgcgtt ctttcctcga caacgctcaa
     1981 caacaagatt ttccactgta ggtataataa aagcacagcg cgatgcgaat gcggaagcgc
     2041 tgtcagcggc aaaatcagag taaacggagt cggtcacatg aatacttgca gcgaagtttt
     2101 cataattacc cacgcggggc ggtccatcgg gggcgggcat gcgagccgct gttccgcgct
     2161 ggccgagggg ttctcttcgc tcggcgttgc tgtccgttgg ctggtgaacg cttccgccgg
     2221 ggagattgtg ctgcgcagag gggctaccga agcgtccgtg acggtgatgg agagtccgtt
     2281 cggcggcggg gcggatgctg tgtttactgc gatctcacgt tctcgtccag ccctctgcgt
     2341 ggtggacggg tacgacgcaa cgccttcttt tctgagggag ctacgccggt tcgccccggt
     2401 ggtgctggtg gacgactgcc gggtacggcc cgtcgagtat gagtgcgacg tggtgctgaa
     2461 ctacaatgtg aacgcggatc tgctcgggta cgaaaaggga catgccgggc tgctgctggg
     2521 gccggagtac gccctgttgc ggagcgagtt ttggagtctg gctcctgaga agggcgatgc
     2581 tgtgctgatc attcccggcg cgagcgattt gctgcatacg ggcgaacggt tcgtggagtg
     2641 gtggggaagc gattggccgc gtgcggagct tgtcctcggt ccgctggtgg agtcttcaat
     2701 ggcggagcga ctgtccgagg ctgccggcgc gttgccgaac ctttcggcag tgcgaaaccc
     2761 gcttgacctt cctgcccgga tggcgaggtc gcgcgcggtg ctgtgcactt ccagcgtgac
     2821 gtcgtacgag gcgctggcgc tgcggaaacc gctggtggtc tttcagacgg cggagaacca
     2881 ggcgggcata gggtgcgaga tagagcgtcg cggcctgggt gtgaacctcg gcgcgtgggg
     2941 aacatggggc ggcgaacggt tgagacgctc gcttgaagat ctccctccgg tgcccccagc
     3001 ctgcgtcaac ccgcgcggcg catgtgccgc cgccgaggca gtattgtgcg ctgtgcggtg
     3061 acggggtgaa atgtcttcgt tgttgtttca ccgaagagca tttcttttgc cggctttatt
     3121 ttatatggat tcatggtaca atagctttta aagcgatgta aatttataaa aatcatatcc
     3181 gaacaggcgg atggttcgcc taaaaaacgt tccgccgggc ggatgtggca gcaagggagg
     3241 ctgaagcatc ctctccgaat ttgaatatat gcaggaggta tgtgcaatga ccatgaattt
     3301 ctttgacgcg gtgaagaacc gcagaacgta ctacagcatt tcaaaaaaga gcccggtctc
     3361 cgacgagcgg atacgcgaga ttgtcgagca ggcggtgaag cactgcccct cggcgttcaa
     3421 ctcccagagc ggcgtgccgt gctgcttttc ggggcggaga gcgacgcgtt ctggagtctt
     3481 gtgaaggagt cgctccggaa gatcgtcccg gcggagaagt ttgcgccgac ggaggagaag
     3541 atcgacggct tcgcctcggg gtacggcacg gtgctcttct tcgaggacga gcgtcccgtg
     3601 aagggtctcc aggagagctt ccccacgtat gccgagcact tccccgtctg gtcgcaccac
     3661 gcgtcgggga tgcttcagtt catcgtctgg gcggggctgg agtcggaggg gttcggagcg
     3721 tcgctgcagc actacgcgcc tgttgtcgag caggcggtga agcagcggtg gggattcccc
     3781 gccgagtgga agctgatcgc gcagatgccc ttcggaacgc cgacggcagg ccccggcgac
     3841 aaggagtttc agccgctgga ggagcgcttc aaggtattca agtagcgttt tccaatcgtt
     3901 ctttcaaggg tgttccgcga ttcctcaagg gcgtggaaca ccctttttcc ttcatctccc
     3961 tctttctacg gatttttccc cgtagaggga gggagaattt tacatttcgg gctctccgtg
     4021 tgaaaatagg tgcgtgcgcc gcggaaacgg gcgcgctgtt gcctgtatga agcacacgat
     4081 gtttgctgat gttgcggcga acttcaatcc gttagtataa aggagcgttt tctgacctat
     4141 ggagactcag tccggcaaag gcccacattt catcgaacag atcatcatgg acgatctgaa
     4201 gaacggcgtc gtcaaggaag tgcacacccg ctttcccccg gagcccaacg gctaccttca
     4261 catcgggcac gcgaagtcga tctgcctgaa cttcgggctg gccgagcagt tcggcggcaa
     4321 gtgcaacctg cgtttcgacg acacgaatcc ggccaaggag gagacggagt acgtcgagtc
     4381 catcatgcgc gacgtccagt ggctcgggtt ccgctggcac acgctccgct tcgcgtcgga
     4441 ctactttcag cagttttacg agtgggcgct ggaactgatt cacgcgggga aggcctacgt
     4501 ggaccatcag acgcccgacg agatccggga gacgcggggc acgctgacgg cccccggcgt
     4561 cgagtccccc tacaggaacc gctccgtcgg ggagaacctt gcgctctttc agcagatgaa
     4621 ggacggcgtg cttgaggacg gaacgtgcgt cctccgcgcg aagatcgaca tgaaccatcc
     4681 gaacatcaac atgcgcgatc cggtgctcta ccggattctg cacagacatc acttccgcac
     4741 cggggacgcg tggtgcatct acccgatgta cgatttcgcc cacggctacg aggacgccat
     4801 cgagggagtt acccactcta tatgcacgct ggagttcgag gaccatcgtc cgctgtacga
     4861 gtggttcctg gacaac
//
LOCUS       DAOSWW010000076         4846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11796_length_4846_cov_4.086621, whole genome shotgun sequence.
ACCESSION   DAOSWW010000076 DAOSWW010000000
VERSION     DAOSWW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11796_length_4846_cov_4.086621"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /gene="rpsQ"
                     /locus_tag="PK442_02510"
     CDS             complement(<1..253)
                     /gene="rpsQ"
                     /locus_tag="PK442_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOO86551.1"
                     /translation="MEERKPHRKIRTGTVVSDKMEKTVVVRVERFEKHPLYGKRIVRA
                     SKYTAHDEKNECRMGDLVTIGETRPLSKTKRWALLEIVRK"
     gene            complement(260..475)
                     /gene="rpmC"
                     /locus_tag="PK442_02515"
     CDS             complement(260..475)
                     /gene="rpmC"
                     /locus_tag="PK442_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOO86552.1"
                     /translation="MESKNLRDLTLDELNEKHKQYKEELFNLRFQNAIGQLKNTSRIK
                     DVRKTIARVLTIVREKQMAGDQSAGRR"
     gene            complement(478..900)
                     /gene="rplP"
                     /locus_tag="PK442_02520"
     CDS             complement(478..900)
                     /gene="rplP"
                     /locus_tag="PK442_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOO86553.1"
                     /translation="MLMPSRVKYRKPFRRPLKGKAKGGNYVAFGEYGLQTLDCAWITA
                     RQIEATRVAISRKMKKGGKIWIRIFPDHPFTKKPLETRMGKGKGAPEFWVAPVKRGRI
                     LFEIAGVPRDIVEEAFRTASHKLPVKVRLVAREGFGGE"
     gene            complement(907..1626)
                     /gene="rpsC"
                     /locus_tag="PK442_02525"
     CDS             complement(907..1626)
                     /gene="rpsC"
                     /locus_tag="PK442_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOO86554.1"
                     /translation="MGQKVHPIGFRLGVASEWESKWFASGKEYAKNLHEDIALRKYIA
                     KKWENAGISRIEIERVGHVIRFTVWTSRPGVVIGRGGAEIQVMKDELQAKTGAKIMIN
                     VQEIKNPDVVSQLVAVGVASALERRVSFRRAMKQSIFRAMKGGAKGIKISCAGRLGGA
                     EIARTEWYNEGRLPLSTIRADIDYGFAEAKTMYGVIGVKVWIFRDKENERPAQAPRQG
                     RGPRQDRSRGDRAPESSRGRG"
     gene            complement(1640..1972)
                     /gene="rplV"
                     /locus_tag="PK442_02530"
     CDS             complement(1640..1972)
                     /gene="rplV"
                     /locus_tag="PK442_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOO86555.1"
                     /translation="MEAKAVARQVRVSPIKVRRVLCLIRGKKVGEALVALRYSPQKSA
                     KLVSKVLQSAVANAEHNFGMDTDKLRIVAATADQGPVMKRFRPVSMGRAHPYRHRTSH
                     VTVIVAER"
     gene            complement(1986..2267)
                     /gene="rpsS"
                     /locus_tag="PK442_02535"
     CDS             complement(1986..2267)
                     /gene="rpsS"
                     /locus_tag="PK442_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOO86556.1"
                     /translation="MARSRKKGPYVDQKLLRKIEDMNEGTAKKVMKTWSRRSTIVPAM
                     IGHTIAVHNGRIHIPIYISENMVGHKLGEFAPTRKFGGHAGQERSSRVR"
     gene            complement(2281..3108)
                     /gene="rplB"
                     /locus_tag="PK442_02540"
     CDS             complement(2281..3108)
                     /gene="rplB"
                     /locus_tag="PK442_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOO86557.1"
                     /translation="MAIKKFNPYTPGRRFMTVQKNDDLTKKDPERSLLEPLHKKGGRN
                     NRGRITMRHRGGGAKRLYRVIDFKRNKLNVPGKVAAIEYDPNRNARIALIHYLDGDKR
                     YIIAPKDLNVGDKIVAGPESDIRPGNALKLKDIPVGTTVHNLELEPGRGAKLVRAAGT
                     SAQLMAKEGKYAYLRMPSGELRLILQECMATIGQVGNEDFENVSFGKAGKTRWLGRRP
                     MVRGMVMNPVDHPMGGGEGKSKSHKHPVSPWGTPAKGYRTRKKKPSDKMIVRRRYEK"
     gene            complement(3130..3426)
                     /gene="rplW"
                     /locus_tag="PK442_02545"
     CDS             complement(3130..3426)
                     /gene="rplW"
                     /locus_tag="PK442_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOO86558.1"
                     /translation="MNLVAHDIIIRPLITEKSSRLMELNKYSFEVHLSANKIQVRKAI
                     EEIFKVKVTAVHILNIHPKPKRMGAFIGKTRSWKKAIVSLAPGERIDFFEGASI"
     gene            complement(3423..4046)
                     /gene="rplD"
                     /locus_tag="PK442_02550"
     CDS             complement(3423..4046)
                     /gene="rplD"
                     /locus_tag="PK442_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOO86559.1"
                     /translation="MPTLKLVSFQGETVGEVQLSDGVFAAPVHVPAMHQVVVAQLANA
                     RLGTQSAKTRGEVRGGGKKPWRQKKTGRARHGSIRSPLWAGGGVTHAPKPRDYSQKVN
                     KKVRRLAIKSALSLKVREELMTVVKSFDIEKPSTKEMLAFLTAVKARKPLIILPEANE
                     AVLKSARNVPGAKVINMVNINVYDLLNAGTLILTPEVVEKLEEVYVG"
     gene            complement(4074..4700)
                     /gene="rplC"
                     /locus_tag="PK442_02555"
     CDS             complement(4074..4700)
                     /gene="rplC"
                     /locus_tag="PK442_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOO86560.1"
                     /translation="MSIGILGKKLGMAQIYNEQGQATPVTIIQAGPCPVVDLKTPSKD
                     GYSALVLGFGEPNPRKLNKSRKGLFEKAEVEPKKTLREFRVDSVDEYAVGQQIDVTLF
                     TEGETINVSGTSKGKGFAGVVKKYHFAGSNASHGASVVHRRGGSSGASSYPGRVFKGK
                     KMPGRMGGEKVTVKNLVVVAVDSDNHLLLVKGAVPGAKNSLVTLFKKG"
     gene            complement(4734..>4846)
                     /locus_tag="PK442_02560"
     CDS             complement(4734..>4846)
                     /locus_tag="PK442_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOO86561.1"
                     /translation="TRTHKRLIDIINPTQKTMDALMQLNLPSGVDIQIKL"
BASE COUNT          839 a         1487 c         1287 g         1233 t
ORIGIN      
        1 ccttgcgtac aatctccaga agcgcccagc gcttcgtctt gcttagagga cgggtctcgc
       61 cgattgttac aaggtctccc atccggcatt cattcttctc gtcgtgagcg gtatacttgc
      121 tcgccctgac gattctcttc ccgtagaggg ggtgtttttc aaaccgctcc acccgaacca
      181 cgacggtctt ctccatcttg tcgctgacga ccgtacctgt ccggatcttg cggtgaggtt
      241 tccgctcttc cattcggggc tacctccttc cggcggactg atcgccggcc atctgcttct
      301 cgcggacgat cgtcaatacc cgcgcgatcg tcttacgtac atccttgatt ctgctcgtat
      361 tcttcagctg tccgatggcg ttctgaaaac gcaggttgaa cagctcttcc ttgtactgtt
      421 tgtgcttctc gttcagttca tcgagtgtaa ggtcgcgcaa attcttgctc tccatgttca
      481 ttcaccacca aaaccttccc gggccacaag acgcaccttg acggggagct tgtgggatgc
      541 ggtgcggaat gcttcttcca ctatgtctct gggaacaccc gcgatttcaa agagaattct
      601 gccgcgcttc acaggagcca cccagaattc cggcgcgccc tttccctttc ccattctcgt
      661 ctccaagggt ttcttggtga aaggatgatc ggggaagatc cggatccaaa ttttgccgcc
      721 ttttttcatc ttacgtgaga tggcgacgcg cgtggcctct atctgtcgcg cggtgatcca
      781 ggcgcagtcg agggtctgga gaccgtactc gccaaaggcg acatagtttc ctcccttcgc
      841 tttcccttta agggggcggc gaaaaggctt tctatacttc actctgctgg gcatcaacat
      901 cgcgagttac ccccttcccc gggaagactc cggggcgcga tctccgcggg aacggtcctg
      961 acggggcccg cgaccctgtc ggggcgcctg cgcaggacgc tcgttctcct tgtcccggaa
     1021 aatccagacc ttgacgccga taacgccgta cattgtcttc gcctcggcga atccgtagtc
     1081 gatgtcggcg cggatcgtcg aaagaggcag acggccttcg ttgtaccact ccgtgcgggc
     1141 aatttcagct ccgcccaaac ggccggcgca ggatatcttg attcccttcg ctccgccctt
     1201 catggcgcgg aagatggact gcttcatcgc acggcggaag gacactctgc gctcaagcgc
     1261 ggatgcaacg cccactgcca caagctgcga cacgacgtca gggttcttaa tctcctggac
     1321 gttgatcata atcttcgcgc ctgtcttcgc ctggagctcg tccttcatca cctgaatctc
     1381 ggcgccgccc cgtccgataa ctacaccggg ccgggaggtc caaaccgtga agcgaatgac
     1441 gtgcccgacg cgctctatct cgatgcggga gattcctgca ttctcccact tctttgcgat
     1501 atacttccga agcgcgatgt cctcgtgaag attcttcgcg tactccttgc cgctggcaaa
     1561 ccacttggat tcccactcac tggcaactcc cagtcggaat ccgattgggt gaactttctg
     1621 tcccacgagt tccccctcct taccgttccg cgactatcac agtcacatga ctcgtacggt
     1681 ggcggtaggg gtgcgcccta cccatggaga ccggacgaaa acgcttcatc actggcccct
     1741 gatccgccgt agctgctact atacgcagtt tgtcggtgtc catgccgaag ttgtgttccg
     1801 cattggccac tgcgctctgc aacactttgc ttacgagttt cgccgacttc tgcgggctgt
     1861 agcggagagc gacaagcgct tctccaactt tcttgccgcg gataaggcag agcacccgcc
     1921 gtacttttat gggagaaacc cgtacctgtc tggcaacggc ttttgcttcc atcaccatta
     1981 cctcctcacc gaacccttga ggaacgctcc tgacccgcgt gtcctccgaa cttgcgggtg
     2041 ggggcgaatt cgccaagctt atgtccgacc atattttcgc tgatgtagat gggtatatgg
     2101 atccgtccgt tgtgcaccgc gatcgtatgg ccaatcatgg cgggaacgat cgtcgatctg
     2161 cgggaccagg tcttcatgac cttcttcgct gttccttcgt tcatatcttc gatcttccgg
     2221 agcagcttct gatccacata gggccccttc tttctggaac gagccattca gtgtccctcc
     2281 ttacttctcg taccggcgcc gaacgatcat cttatcggac ggcttcttct tccgggtgcg
     2341 gtatcctttt gcgggcgttc cccaagggga gacgggatgc ttgtgcgact tgctcttgcc
     2401 ttcgccgccg cccatggggt ggtccacagg gttcatcacc ataccgcgaa ccattggacg
     2461 tcttccaagc caacgggtct tcccggcctt gccgaaggac acgttctcaa agtcttcgtt
     2521 accgacctgg cctatcgtcg ccatgcactc ctgaaggatg aggcggagct caccgctggg
     2581 catacgcagg tacgcgtact tgccttcctt cgccatgagc tgtgccgaag ttccggcggc
     2641 acggacgagt ttcgctcctc tgccgggttc gagctcaagg ttgtgcacgg tggttcccac
     2701 cgggatgtct ttcagcttca gcgcgttgcc cgggcggatg tccgactcgg ggcccgcgac
     2761 gatcttgtcg ccgacgttca gatccttggg ggcgatgata tatcgtttat cgccgtcaag
     2821 gtagtgaatg agcgcgatcc tcgcgttcct gttcggatcg tactcgatcg cggcaacctt
     2881 cccgggaacg ttgagcttgt tcctcttgaa gtcgatgact ctgtagagtc tcttcgcgcc
     2941 gccgcctctg tggcgcatgg tgattctgcc gcgattgttc ctgccgccct tcttgtgaag
     3001 aggctcgaga agagagcgct ccggatcctt tttggtaaga tcgtcgttct tctgaacggt
     3061 catgaacctg cggcctggag tataggggtt gaacttcttg atcgccattg gtttcctttc
     3121 tccttcccgc taaatgctcg cgccttcgaa gaagtcgata cgctctcccg gagcgaggct
     3181 gactatcgct tttttccatg aacgggtctt tccgataaaa gcgcccattc tcttcggctt
     3241 cggatggata ttcaaaatat gaacggccgt tactttgacc ttgaagatct cctcaatcgc
     3301 cttgcgaacc tggatcttgt tcgcggacaa atggacttca aagctgtact tgttcagctc
     3361 cataaggcgg ctgctctttt cggtgatcag cggacggatg atgatgtcgt gcgctacgag
     3421 gttcatccga catacacctc ctcaagcttt tccacgactt ccggggtcag gatcagcgtc
     3481 ccggcgttga gaagatcgta gacattgatg ttcaccatgt tgatcacctt cgcgcccggt
     3541 acgttgcggg cggacttcaa tacggcttcg ttcgcctcgg gaagaatgat gagcggtttc
     3601 ctggctttga ccgccgtgag gaacgcaagc atctccttcg tggagggctt ttcgatgtcg
     3661 aacgatttaa cgaccgtcat aagctcttcg cggactttca gcgacagcgc gcttttgatc
     3721 gcgagacggc gtactttctt gttcaccttc tggctgtaat ccctgggctt gggggcgtgc
     3781 gtaacgccgc cgccggccca caggggcgaa cggatgctcc cgtgacgggc gcgtcctgtc
     3841 ttcttctgtc tccagggctt cttgccgccg ccccggactt ctccccgcgt cttcgcggac
     3901 tgcgttccga gtctcgcgtt cgcaagctgg gcgacgacta cctggtgcat ggcgggtacg
     3961 tgcacggggg cggcgaaaac gccgtccgaa agttgcactt cccctaccgt ttctccctgg
     4021 aagctgacga gtttcagggt aggcatgtta tttccggcct ccttctctgc cgactacccc
     4081 ttcttgaaaa gggtcaccag gctgtttttc gcgcccggta ccgcaccctt gacaagaagc
     4141 aggtgattgt cggagtccac tgcaacgacg acgagattct tgactgtaac cttctcgccg
     4201 cccattctac cgggcatctt tttgcccttg aaaactcttc cggggtagct gcttgcgccg
     4261 ctggagccgc cgcggcggtg caccacggac gcgccgtggc ttgcgttcga accggcgaaa
     4321 tgatacttct tcacgacgcc cgcgaagccc ttacctttgc tcgtaccgga gacgttgatc
     4381 gtctcgccct ctgtgaagag agtgacgtcg atctgctggc cgacggcgta ctcgtcgaca
     4441 gagtcgaccc ggaactcgcg aagcgtcttc ttcggctcca cttcagcctt ctcgaacaga
     4501 cccttgcggg acttgttcag ctttctcgga ttgggctcgc caaatccaag gacgagtgcg
     4561 ctgtatccat ccttcgaggg cgtcttcaaa tccaccacgg ggcatggtcc ggcctgtata
     4621 atcgtcacag gcgtggcttg gccctgctcg ttgtaaatct gggccattcc gagttttttc
     4681 cccagaatac cgatgctcat tacactccaa ctcctttcag ctcagagctt cccctacaat
     4741 ttaatctgga tatccactcc agaaggaagg ttcagctgca taagcgcgtc catggtcttc
     4801 tgcgtcgggt tgatgatgtc gatgaggcgc ttatgtgtcc tggttt
//
LOCUS       DAOSWW010000077         4826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11873_length_4826_cov_3.237686, whole genome shotgun sequence.
ACCESSION   DAOSWW010000077 DAOSWW010000000
VERSION     DAOSWW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11873_length_4826_cov_3.237686"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..732)
                     /locus_tag="PK442_02565"
     CDS             complement(193..732)
                     /locus_tag="PK442_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOO86562.1"
                     /translation="MGELIAQARGVAIFPSVVKAGFVFGGQYGEGLVLRRDPVKKRWY
                     GPSFVSVTGASWGLQIGAQSIALVLVITNDRGLEGFMGNNVKLGGDLSVAAGPVGRRG
                     EMGTDYKMKASIYSYSMTKGLFAGVSLEGAAINVDRNANQAYWGKPFSVGNALNSAAT
                     KNEIKQLVIELEKIMKRAK"
     gene            1031..1960
                     /locus_tag="PK442_02570"
     CDS             1031..1960
                     /locus_tag="PK442_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86563.1"
                     /translation="MNTTQKIERLLDEAYTTDDPEEMERLARQILNYDKDNVEAIILL
                     ADVTEDSKEKIVLLQKASCLLEKDLEESPVPEGTAFLDDELGMLYAAVLQRLGFSYFS
                     EGENEKALEIAHSIMAFDTEQQTLGRTLYYRILVDMNEYTAILEECLKEREPFPAMLH
                     SKAIAAFRLSGATMPAYRALWEAFAADPDVPFHILGYYEEPDEEAEEEELESYSLAML
                     FEDAWLTEGDAALANWVAGGTILLGLAASLFPAENSEKMLVLADALGLADAAEDIMVK
                     LESRQDWNVLSKQEKVKEALKLLSEGKYLPLKS"
     gene            2225..2704
                     /gene="nuoE"
                     /locus_tag="PK442_02575"
     CDS             2225..2704
                     /gene="nuoE"
                     /locus_tag="PK442_02575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOO86564.1"
                     /translation="MVVETKQDVRSMVEAITAPWKGKHGGVIPVLQETQKQVGFLSRE
                     AMEVVSEELGVPLAELYGVATFYAQFHLKPRGRHIIRVCRGTACHVRGSAKIMDKIMS
                     MLKVKENGTTDDLRFTLEPVACLGCCGLAPVVMIDEDTHGRLTDDEAALQEFLDQYV"
     gene            2707..3249
                     /locus_tag="PK442_02580"
     CDS             2707..3249
                     /locus_tag="PK442_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86565.1"
                     /translation="MLEDLSHHILDIAENGVNAGAESIGVTFDDDGEFIRLTVRDNGR
                     GMSEATARAVVDPFYTTRTERRVGLGLPFLKQLSELCGGEFLLSSAPGKGTTVSASFS
                     KQCVDTPPPGDIPSTLLTLLVGHGHISWKYVHRVGKREFLLDSDELKDALGGGNPFTD
                     PALAVGVKEYIAENIGELYM"
     gene            3287..3673
                     /locus_tag="PK442_02585"
     CDS             3287..3673
                     /locus_tag="PK442_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOO86566.1"
                     /translation="MTEEKKKIRSLDDLRKIKEKTGDLTSARSEVDKVKVIIGMGTCG
                     IAAGAREIMNAVLKELEVRNLHNVAVETTGCIGMCQNEPLLDVIRPGEPRITYCAVTP
                     PDVSRIVSEHIVNGRIVQDKLYGRAD"
     gene            3692..>4826
                     /locus_tag="PK442_02590"
     CDS             3692..>4826
                     /locus_tag="PK442_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOO86567.1"
                     /translation="MALYRAHVLICHGTGCASGGAPKVLDAFKEELAKKGLEKEILVI
                     ATGCHGMCEFGPIVVVYPEGTFYSQVKPSDVAEIVEEHLYKGRIVTRLLYKEPLSAQK
                     VCHYKDIPFYGKQHRIVLSNCGYINPDNIDEYISRDGYQALGKCLVEMTPAAVIDEMK
                     KSGLRGRGGGGFPTGMKWGFCAASPGDKKYVICNADEGDPGAFMDRSILEGDPHAVIE
                     GMMIGGYAMGADEGYIYCRAEYPLAINRLQTAIKNATEYGLLGEDILGTGFNFTLHIK
                     EGAGAFVCGEETALMASIEGQRGMPRARPPFPAVKGLWGKPTNINNVETWANVPRIIV
                     QGAECFAAMGTEKSKGTKVFALTGKVKNTGLVEVPMGTSTSPVF"
BASE COUNT         1257 a         1157 c         1283 g         1129 t
ORIGIN      
        1 tgagcgcaag gtgcaagagg ctatggccaa acagcaggct cagaaaccga agatagacgt
       61 cgcctctccg gctgttctac aacagctgaa cagaatggga ggaaaagggc agggcggagg
      121 aaagcttatt atctaactgt acgatatgct gcgggcgcgg aatcgaagcg ccggcagcat
      181 atcttttcga gcttatttcg cccgtttcat gattttttcc agttcgatga cgagctgttt
      241 tatctcgttt tttgttgcgg ccgaatttaa ggcgtttcct actgaaaacg gtttgcccca
      301 gtacgcctgg ttcgcattcc tgtccacatt gatagctgct ccttcaagag agacacccgc
      361 aaacaaaccc tttgtcatcg aataactata aatcgaggct ttcatcttat agtcggttcc
      421 catttcacct cttcgtccca caggtccggc ggctaccgag agatctcctc ccagcttgac
      481 gttgtttccc ataaagccct ccagccccct gtcgttggtg attacgagaa caagcgctat
      541 ggactgcgct ccaatctgta atccccagga ggccccggta acgcttacga aggatggacc
      601 gtaccatctt ttttttaccg ggtcgcggcg aaggacaagg ccctcgccgt actgtcctcc
      661 gaaaacgaac ccagctttta cgacggaagg gaaaatagcg actcccctgg cctgcgcaat
      721 aagctctccc attgtcgatg catcatcctg tttcgacatt tcacgaagca cttttacgga
      781 atcggcaatt cgaccttttg gagttgaagc gaacgatggc ctttccgtca aattcaagat
      841 aagtaacaag aagacaacac acaaagaaaa agatacgcgc acccgtctca ttccctctcc
      901 tcctagaaaa aaaattcatg gttttttgca cttcttcata ttctacacca acctatttga
      961 ccgggaaaaa atcgtgagct agacttcttg gagagggaag agactgaaaa agcaaacgga
     1021 ggaaatatct atgaatacta cacagaaaat cgaaagactt cttgacgagg cgtatactac
     1081 cgatgatccg gaggaaatgg aacgcctcgc tcgacaaatt ttgaattacg acaaagataa
     1141 tgtggaagca atcatccttt tggccgatgt caccgaggac tcgaaggaga agatcgtcct
     1201 tttgcaaaaa gcaagttgct tattggagaa agatctggaa gaatcgcccg ttccggaagg
     1261 aacagccttt ctggatgacg aactcggaat gctctacgca gcagttcttc aacgcctcgg
     1321 tttttcctat ttctccgaag gcgagaacga aaaagcgctt gaaattgcgc actcaatcat
     1381 ggcatttgac acggaacaac agacgctggg aagaactctt tactatcgta tccttgtcga
     1441 tatgaacgaa tacacggcga tccttgagga gtgcttgaaa gaaagagagc cttttcctgc
     1501 aatgctccac agtaaagcta tagctgcgtt taggctctcc ggagctacca tgccggccta
     1561 tagggcgctt tgggaagcgt tcgcggcgga tcccgacgtt cccttccaca ttctcggcta
     1621 ctatgaagaa ccggatgaag aggctgaaga ggaggaactg gaaagctaca gtctcgcaat
     1681 gctctttgaa gatgcgtggt tgactgaagg agacgccgcg ttggcaaatt gggttgccgg
     1741 agggacaatc ttgcttggtc tggccgcatc gctcttcccg gcagagaatt ccgagaaaat
     1801 gctggtgctt gcagatgccc tcggtctcgc cgacgctgct gaggacataa tggtaaagtt
     1861 ggagagcaga caggactgga atgttctttc aaaacaggaa aaagtgaagg aggcattaaa
     1921 gctgctctct gaaggtaaat atcttccact gaaatcttaa tgctcttatt ttttattgaa
     1981 ttagatgtgt ttttgtaaaa aaggatccag caagaagact acgtatcgag aagatgtatg
     2041 agataaagca aaaacttgct tttttagaga aaaaatcttt tttctgtggt aatatttaca
     2101 tcgggcgccg tttacaggaa aacggtttct ttccccgaag ttgttttttt gtgtagaatc
     2161 aacaagagat agttttttgt tattctatat gttattaaat tcactatact tagggggtcg
     2221 ggcaatggtt gtggaaacaa agcaggacgt gcgttccatg gtagaggcaa ttaccgctcc
     2281 gtggaaaggg aagcacggag gcgttatacc ggtactccag gagacacaga aacaagtagg
     2341 attcttgtcc cgggaggcta tggaggttgt gtccgaggaa ctgggcgttc ctttggcgga
     2401 actttacggc gtggcgactt tttatgccca gttccatctg aaacccaggg gacgccatat
     2461 tatccgagtg tgccgcggaa cagcgtgtca tgttcgcggc agcgcaaaga tcatggacaa
     2521 aatcatgtcg atgctcaagg taaaggaaaa cggaaccacc gatgatcttc gttttacact
     2581 tgaaccagtc gcgtgtcttg gttgctgcgg ccttgctcct gtagtgatga tcgacgagga
     2641 tactcacggc agactcaccg acgacgaagc ggccttgcaa gaattccttg atcagtacgt
     2701 ctgatcatgc ttgaggatct ctcccaccat attctcgata tcgctgagaa tggcgtgaat
     2761 gccggagccg aatcgatcgg tgttacattt gacgacgacg gcgagttcat tcgtttgacc
     2821 gttcgcgata acggcagggg aatgagcgaa gcgacagccc gcgctgtagt ggatcctttc
     2881 tacacaacgc ggacggagcg ccgggtgggt cttggacttc ctttccttaa acaactttct
     2941 gagttatgcg gtggtgaatt ccttctttct tcagcgccag gaaaaggaac aactgtttcg
     3001 gcgtcattca gcaaacagtg cgtagatact cctcctcccg gagatattcc ctcgactctg
     3061 cttactcttc ttgtcggtca cgggcatatc tcctggaaat atgttcacag agttggcaag
     3121 agggagttcc ttctggacag cgatgaactg aaggatgcgc tcgggggcgg gaaccctttc
     3181 acagatcccg cgcttgccgt tggagtaaag gagtacatcg cggaaaatat cggcgagctt
     3241 tatatgtgaa aaaaatggga aataatgcgg acaggaggaa aaagaaatga ctgaggaaaa
     3301 gaaaaagatc cggagtctcg acgatctgcg aaagatcaaa gagaaaacgg gcgatttgac
     3361 atccgcgcgt tccgaggtgg acaaagtcaa ggtcatcatc ggtatgggta cgtgcggtat
     3421 cgccgccggc gcccgcgaaa taatgaacgc ggtgcttaaa gagcttgaag ttcgcaatct
     3481 gcacaacgtc gccgtagaga cgacagggtg catcggcatg tgccagaatg agccgcttct
     3541 ggatgttatc cgtccgggcg agccgaggat aacctactgc gctgtcaccc ctcccgacgt
     3601 ttccaggatt gtctcggagc acatcgtaaa cggccgcatt gtgcaggaca agctgtacgg
     3661 cagagccgac taagcgggga gggataagga tatggcattg taccgcgcgc acgtactcat
     3721 ctgtcacgga acaggatgcg cttccggcgg cgccccgaag gtgcttgatg cgtttaaaga
     3781 agagcttgca aagaaaggtc tcgaaaaaga gatccttgtt attgcaaccg gctgccacgg
     3841 catgtgtgaa ttcggtccga tcgtcgtggt ctatccggaa ggaacgtttt actcgcaggt
     3901 taagccttca gacgttgcag aaatcgtcga agagcatctg tataagggcc gcatcgtcac
     3961 acgtcttctt tacaaagaac ctctgtccgc tcagaaggtc tgtcactaca aggatattcc
     4021 gttctacggt aaacagcata gaatcgtatt gagcaactgc gggtatatca acccggacaa
     4081 tattgacgag tacatctcca gagacggata ccaagctctg ggaaagtgtc ttgtcgagat
     4141 gaccccggca gcggtgattg acgagatgaa aaagtccggg cttcgagggc gtggaggcgg
     4201 cggtttcccg acaggaatga aatggggttt ctgcgcagcg tcccccggcg ataagaaata
     4261 tgtcatctgc aatgcggacg agggagatcc gggcgcgttc atggatcgct ccattcttga
     4321 gggcgacccc cacgccgtca tcgaaggtat gatgatcggc ggctacgcca tgggcgccga
     4381 cgaaggatac atctattgtc gtgctgaata ccccctggca attaatcgcc tgcagaccgc
     4441 tataaagaat gccaccgaat atggcctgct gggcgaagac atcttaggaa cgggcttcaa
     4501 cttcaccctt cacatcaagg agggcgccgg agctttcgtc tgcggcgaag agaccgcgct
     4561 tatggcctcc atagaaggac agagaggcat gccgcgcgcc cgtccgcctt tcccggctgt
     4621 caaaggactc tggggtaagc cgacgaatat caacaacgtc gagacgtggg cgaacgtccc
     4681 aaggattatc gtccagggag cagagtgctt tgccgcaatg ggaaccgaga agtccaaggg
     4741 aaccaaggtt tttgcactca ccggaaaggt caagaatacc ggacttgtcg aagtgcccat
     4801 gggcacttcg acaagtccgg tattct
//
LOCUS       DAOSWW010000078         4825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11875_length_4825_cov_3.972537, whole genome shotgun sequence.
ACCESSION   DAOSWW010000078 DAOSWW010000000
VERSION     DAOSWW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11875_length_4825_cov_3.972537"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK442_02595"
     CDS             complement(<1..201)
                     /locus_tag="PK442_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86568.1"
                     /translation="MITKQFSRPDKDFVFFFPFEFSSCKIFIQRRPYERDFLENRFAS
                     LDLTVRGIVITRSPVTRFLFVRE"
     gene            115..1491
                     /gene="ricT"
                     /locus_tag="PK442_02600"
     CDS             115..1491
                     /gene="ricT"
                     /locus_tag="PK442_02600"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOO86569.1"
                     /translation="MDEDLAAAELEREEENEVLIRARKLLRNHNLSMKLVDVEYLLDK
                     KKLFFYFTSEQRVDFRAYVRDLAKEFKTRIELRQIGVRDEAKTVKGIAPCGRECCCSH
                     WLHRFTPICIKMVKEQNLALNPTKISGICGRLMCCMSYEHTSYSSLWKTLPNPGAKIR
                     TPAGNYIVEGVELSSNSVRVRRPDGGSLLIVIEDFPRFKETVLEGKEWEDSSRTPRDF
                     SSAKTLFDSPASKGTRSFSDDSSRTPRKRAAESGGHEKVQEQGSLAKEKKTEGKPAVS
                     GTEKNKREKEKKLQEGLLAPEEVAKPAKKKRRRKKPSASGDNKTTPVGTQQPRQQERG
                     DGKQTESKYSEEASSGGRPSRKHASPELNQSNLSAEKTDGAPHSPENTTAPSARRSSS
                     SRRRRSGKGGGNRDTHEQNKSPEQKTEGSESKNASSGGAQQKRQKNKPHPRPKEESGE
                     SRKEKGPE"
     gene            1527..2468
                     /gene="ftsY"
                     /locus_tag="PK442_02605"
     CDS             1527..2468
                     /gene="ftsY"
                     /locus_tag="PK442_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOO86570.1"
                     /translation="MSFFEKLKKSLQGVRKKWSSGLSGIFSGSAIDEEFWDRLEEVLI
                     SGDVGVTLSLRLIEDLKKEARARGAKTPEELFQFFADILGARLSAVPLMGQPVTPDKN
                     SLTIVILVGVNGSGKTTTAGKLASQWTKEGFRVVFAAADTFRAAAIDQLKVWGERSGV
                     RVIAQQQGSDAAAVAFDALSAAKSSRAHVLLVDTAGRLHSKHNLMEELGKIYRVLEKE
                     RGDAPLETLLVLDAVMGQNGLAQAQTFNKILPLSGVVLSKYDNTAKGGILLAIAEELK
                     MPVRYVGLGEGEDDLRPFDIHDFISALLEGDKNAGRE"
     gene            3251..3814
                     /locus_tag="PK442_02610"
     CDS             3251..3814
                     /locus_tag="PK442_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOO86571.1"
                     /translation="MSVSTTDKPSRNTAYFYPVKLIVGVLYPDEARWTWTKNALAALW
                     GSPEDESAPYLFSSLTDYYADIAPVLFRRFLSFQTLRDAGELQEWKISACETEKRSGT
                     PRKVNVDPGYVNGARLVLASTKDHAHRIYIGGGIHAEVTLRYRRKRWTPFDYTFPDFA
                     DGRYNDFLSLVRKRWLDEMTARRLRND"
     gene            3807..>4825
                     /gene="purB"
                     /locus_tag="PK442_02615"
     CDS             3807..>4825
                     /gene="purB"
                     /locus_tag="PK442_02615"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOO86572.1"
                     /translation="MIDRYTTKEMNAIWSEENKFRTWLEVELAVCKVWNERGVIPSEA
                     YDEICAKADFRIERINEIEEQVQHDVIAFVSAVAETVGKNGRYIHLGLTSSDVIDTAS
                     SLSLKRSLTVVDEKVVQLISAVNKRAEEFRYTPCAGRTHGIHGEPTTFGLKLMNWQYQ
                     LQRDRQRIALAVEQINYGKISGAVGTYAHCDPDLEKRVCDILGLRPALVSTQILQRDR
                     HANILNTLALLGSALDRFATEIRHLQRTEVMEVLEPFGARQKGSSAMPHKRNPILCER
                     ISGMSRLLRGYALTGMENINLWHERDISHSSAERVVWPDAFHTICYMLSKMKSVVENL
                     RVDTE"
BASE COUNT         1324 a         1214 c         1261 g         1026 t
ORIGIN      
        1 ctctcggacg aacaagaagc gcgttaccgg gcttcttgta atgacgatgc ctctgacggt
       61 caggtcaagg gaggcgaacc gattctccag gaaatctcgc tcgtacggac ggcgctggat
      121 gaagatcttg cagctgctga actcgaacgg gaagaagaaa acgaagtcct tatcagggcg
      181 agaaaactgc ttcgtaatca taacctctct atgaagcttg ttgacgttga atatcttctt
      241 gacaagaaga aactcttttt ctattttacg tccgaacaaa gagttgactt ccgtgcgtat
      301 gtccgggatc tggcgaaaga attcaaaacc cgtatcgaac tccgtcagat aggcgtgcgc
      361 gacgaggcaa aaacagtgaa agggatcgcg ccgtgcggca gagaatgttg ctgcagccac
      421 tggcttcaca ggttcacacc aatctgcata aagatggtaa aggagcagaa tctcgctctg
      481 aatccgacga aaatatcggg catctgcgga aggctgatgt gttgcatgtc ctatgagcac
      541 acatcgtaca gcagcctctg gaaaacgctc ccgaatccgg gtgcaaaaat ccgcactccc
      601 gccggcaatt atatcgttga gggagttgag ctctcatcca actccgtacg cgtacgccgt
      661 ccggacgggg gaagccttct catcgtaatt gaagattttc cgcgtttcaa agagactgtc
      721 ttagaaggga aggaatggga ggactcttct cgtacgcccc gtgatttctc atcggcgaag
      781 actctttttg acagcccggc gtcaaaaggc acacgttcat tctcagacga ctcgtcccgc
      841 actcctcgca agagagcagc tgaatccggc gggcatgaga aagtgcagga acaaggctct
      901 ctcgcaaaag agaaaaaaac ggaaggcaaa cctgcggttt ccggaacaga aaagaacaaa
      961 cgagaaaaag agaaaaagct tcaggaaggt cttcttgcac ctgaagaggt agccaaaccg
     1021 gcgaaaaaaa agagacgaag aaagaaacct tcagcttccg gcgacaacaa aaccacaccg
     1081 gttggaaccc aacagccgcg tcaacaggaa cgcggagacg gaaaacaaac agagtcaaaa
     1141 tactcggaag aagcctcttc cggtggtcga ccgtccagga aacatgcgtc gcctgagctt
     1201 aaccagagca atttatcggc agagaaaacc gatggagctc cccattctcc tgagaatact
     1261 accgcccctt ccgccagacg gtcttcgagt tccagaagaa gacgttccgg aaaaggcgga
     1321 ggaaacagag acacacacga gcaaaataag tctccggaac aaaaaactga aggatccgaa
     1381 tcaaagaacg cttcctcggg cggagcgcaa caaaaacgac agaaaaacaa accgcacccc
     1441 cgccctaaag aagaatctgg agaatcccgc aaagaaaagg ggccggaata acggctgcac
     1501 gtcccgagga ggaggcctga cagtacatgt ctttctttga aaagctcaaa aaaagtctgc
     1561 agggagtacg caaaaaatgg agcagcggac tctccggaat tttttcgggc agcgcaatag
     1621 atgaggagtt ctgggatcgt ctcgaagaag tccttatctc aggagatgta ggcgtcacgc
     1681 tctcactgcg tctgattgag gatttgaaaa aagaggcgcg tgcacgcggc gccaagacgc
     1741 cggaagagct ttttcaattc ttcgcggaca tcttaggagc tcgcctttct gctgttcctc
     1801 tgatgggaca gccggtaacg ccggacaaaa attcgttgac catagtcatt cttgtcggcg
     1861 tcaatggaag cgggaaaacc acaacagcag gtaaactcgc ttcccaatgg acgaaagaag
     1921 gcttccgagt tgtctttgcg gctgccgaca cgtttcgtgc cgccgcgatt gatcaattaa
     1981 aagtatgggg ggagcgttcg ggagtacgcg tcatcgcgca acaacaaggg agcgatgcag
     2041 ccgcagtagc ctttgacgca ctctctgccg caaaatcgag ccgtgcgcac gttcttcttg
     2101 tcgatactgc cggacgtctt cactcgaaac ataaccttat ggaagaactc ggaaagattt
     2161 acagagtgct cgaaaaggaa cggggcgacg ctcctctcga aacgctgctc gtactggatg
     2221 ccgtgatggg acagaacgga ctggctcagg cgcagacgtt caataaaatt ctgcctcttt
     2281 caggcgttgt cctctccaag tacgacaata ctgcgaaagg cggcattctc cttgctatag
     2341 cagaggagtt aaaaatgccg gtacggtatg taggccttgg agaaggggaa gacgaccttc
     2401 gtccattcga tatccatgat tttatttcag cgcttctgga gggagataaa aatgccggac
     2461 gagaatagag cagaagtgcg atacaacact acgctcgaat ctcctctgtc gtcgcttctt
     2521 cgctccctct atctgcacgg agttgagcat tgcgcgcttg cgacatctca aggtttttca
     2581 ctctactcga aggatcagat tgtctctctt cttgaaagag gaaacgccga tacaaaagtc
     2641 ggcgatgcaa tcgcgttact ttcatcaaac ggtttcgcct gcctcgctga acagcgtcta
     2701 atggaacctt taactccggt tttactggtt actgacaggg gtgttgagct cgttcccgcg
     2761 ggaaaaattc cgcaaccttc ccccaaacaa gaatgcacag ctccggacga gggaacaacg
     2821 gactcaggcc tcccatgctg gtggacaata ccgcttcctc tcgctttctt aacggggaaa
     2881 aaaccggttt taaaccctgc tgccgacaaa ctcctcgacg ggtttacact gccttctttc
     2941 gcccggaaaa aaaagcaccc cactgagttc ctcatcgagc tcggcagcaa atcaacgaaa
     3001 aagcgaaata ctatctcaag gcattttctg ttcactgagg tgaaggactc ttttttcctg
     3061 atccaggagg ttacggaaga tctgcagagt gcggaacaaa tggcatggct tgcttccatc
     3121 ggccaagcat ttctttccga tctcaagtgc aaaggaatac cggtccggca gattcgttcg
     3181 cccgaatata cagccgaaga agagaacgga aggcgcctgg cgtgcatatg ggaggaaaaa
     3241 cttcttggat atgtctgtat cgacaacgga caaaccgagt aggaacaccg cgtacttcta
     3301 tcctgtgaag ttgatcgtcg gggttctcta ccccgacgaa gctcgttgga cgtggacaaa
     3361 gaatgcgttg gcagccctgt ggggatctcc cgaagatgaa agcgctccgt accttttttc
     3421 ttcgctcact gattattacg ccgacatagc tccggttttg tttcgacgtt ttctctcgtt
     3481 ccaaactctc cgggatgcag gagagctgca ggaatggaaa atttccgcat gtgagacgga
     3541 gaagaggagc gggacgccga gaaaagtaaa cgtcgatccc gggtatgtga acggagcgag
     3601 actcgttctt gcctctacga aagaccatgc gcacagaatc tatatcggcg gggggatcca
     3661 tgcggaggtg acgttgcggt acaggcgaaa gcgatggacg cccttcgatt acaccttccc
     3721 cgattttgcg gacggacgtt acaacgattt cctctcgctt gtacggaaac gctggctcga
     3781 tgaaatgact gcgaggaggc tgcggaatga ttgacagata tacaaccaaa gaaatgaatg
     3841 ctatttggag cgaggagaac aagttcagga catggctaga agttgaactc gccgtatgca
     3901 aagtgtggaa cgaacgcggc gttatcccgt ctgaagcgta cgacgaaata tgcgcgaaag
     3961 cggattttcg tattgaacgg atcaacgaga tcgaagaaca ggtacaacac gacgtcattg
     4021 ctttcgtctc agccgttgcg gagaccgtcg ggaagaacgg ccgctatatt catctaggac
     4081 tgacgagcag cgatgtcatc gacactgctt cttctctttc actcaaacgt tctctgactg
     4141 tagtggacga aaaagtagtc cagctgattt cggctgtaaa caaacgcgcg gaagagttcc
     4201 gatacacgcc gtgcgccggc agaacacatg ggattcacgg agagccgact actttcggat
     4261 tgaaactgat gaactggcaa tatcagcttc aaagagaccg ccagcgaatt gctcttgcgg
     4321 ttgagcagat aaattacgga aaaatctccg gggcagtggg cacatacgca cactgcgatc
     4381 ccgaccttga aaaacgcgtt tgcgacatac tggggcttcg gcctgccctc gtttccacac
     4441 aaattctcca gcgcgaccgc catgcgaaca ttttgaacac gctcgctctt cttggaagcg
     4501 ccctcgaccg ttttgcaacg gagatccgtc atcttcagag aaccgaggtt atggaagtcc
     4561 tggaaccttt cggcgcacgg cagaaagggt cgagcgccat gccgcataag cggaacccga
     4621 ttctctgtga acgtatttcc ggtatgagca ggctgcttcg aggctacgcg ctcacaggaa
     4681 tggaaaacat caatctctgg cacgaacggg atatcagtca ctcgtccgca gaaagagttg
     4741 tctggcccga cgcttttcac acaatttgct atatgctttc gaaaatgaag agtgtagttg
     4801 aaaatttacg agttgatact gaaaa
//
LOCUS       DAOSWW010000079         4819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11902_length_4819_cov_3.758816, whole genome shotgun sequence.
ACCESSION   DAOSWW010000079 DAOSWW010000000
VERSION     DAOSWW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11902_length_4819_cov_3.758816"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..800
                     /gene="thyX"
                     /locus_tag="PK442_02620"
     CDS             105..800
                     /gene="thyX"
                     /locus_tag="PK442_02620"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOO86573.1"
                     /translation="MRCGVLLLSFTPRPDAVVAAAAKLCYSDVSAADLLSDESSEKMR
                     GLLRHIRASGHFSPFEHASFTFAVDGLSRVTSHQLVRHRIASFSQQSQRYVTMGEPDV
                     VVPPSIAERPELTDGFLEMTRSAHAFYRKLVESGVPKEDARYILPHGWSTRLVMTMNA
                     RELHHFFTLRLCRRAQWEIRNLAREMLLAVREAAPLLFETAGPSCLVRGRCEEKSPCG
                     RPFASLEELLDDN"
     gene            814..1752
                     /locus_tag="PK442_02625"
     CDS             814..1752
                     /locus_tag="PK442_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOO86574.1"
                     /translation="MKRLFASMLLFLAEGLEQKRIPVGGQAVIEGVLMKGPERWGLSV
                     RKPDGEIENETWQSFSRTKRMPWKLPIVRGVVTMVEMMTVGFRALNKSAQIAVGEEEK
                     ITPKEFLLSVLVAIGAVVGLFIALPLWLSDLAVTSWGLTQTGKNILEGCVRGLVFIAY
                     VGGIGLWSEIRQVFRYHGAEHKTINAFESGADMTPENIRKFSRIHPRCGTSFLLIVVA
                     VSIVVFSIAGSGSILWRIDSRIVLLPLVMGISYEIIKGASCAGILGRILMAPAMSFQY
                     LTTREPDEKQIEVALTSLETALGRKLSPVAAEAPAE"
     gene            1749..2825
                     /gene="prfA"
                     /locus_tag="PK442_02630"
     CDS             1749..2825
                     /gene="prfA"
                     /locus_tag="PK442_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOO86575.1"
                     /translation="MNFQDKLSELERDFEETEAKMADPAFHSNMKELQGLAKRHSKLA
                     PVVSKFREFKKVTVDLEEARSLLHCGDPEMESLAKDELSSKEDRLREIEEEMKLLLLP
                     EDPNDEKSVIVEIRAGTGGEEAALFAANLFRMYTRFAEREGWRTEIVDYNETGIGGYK
                     EIIFRIDGDGVYSKLKFESGVHRVQRVPATEAAGRIHTSAATVAVLPEVEDVDVEIRT
                     EDLKIDTYRSSGAGGQYVNMTDSAVRITHLPTGVVATCQDERSQLKNRVKAMAYLRAK
                     LYDLELQKQNDEVASERRGQIGSGDRSERIRTYNFPQNRISDHRIGMTLYKLDQYLDG
                     DLYELVDAMTMADQSQKLHAIEAN"
     gene            2827..3672
                     /gene="prmC"
                     /locus_tag="PK442_02635"
     CDS             2827..3672
                     /gene="prmC"
                     /locus_tag="PK442_02635"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOO86576.1"
                     /translation="MTATEARRRLVRELSAAGVFSPGLEADVILSVACGVSSALLRAR
                     PDFELTEENVSELARITGRRARREPLQYILGSWEFYGRALETPPGVLIPRPETEVLVE
                     KALERLPPEGGVFLDWGTGSGCIALSLLCENASACAIAADCNPRALRVSWTNMKRWGV
                     LRRCLLWHSRTPDDLPVSPASLDMLVSNPPYIPTDRMNTLMEEVRYEPQSALDGGIDG
                     ADCYRRLFIAGERMLRRGGSLLFEAGDGEQIAFLAGIAPECFELDGIFEDFSKSPRVI
                     AWRRV"
     gene            3718..>4819
                     /gene="trxB"
                     /locus_tag="PK442_02640"
     CDS             3718..>4819
                     /gene="trxB"
                     /locus_tag="PK442_02640"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOO86577.1"
                     /translation="MEKRELVIIGAGPAGLSAAIYGRRAGLDVLLLEKGVPGGQINIT
                     EEIENWPGVEHATGPELGRMFRSHAEKFKTEFRDVDVSKVEIRGDAKIVVTNKGEIEA
                     EAVIIATGAYFRRLGCEGEAEHIGQGVSYCAVCDGAFFEGEEIAVVGGGNTAVEEGVY
                     LTNFASKVFIIHRRDEFRADRAAVEHAMSNPKIVPVWNTVVEKIEGDGMVENLVLKNL
                     KTGEVSNLPVAGVFMFVGQSPHDECIRGLVDASKGGWIKTNDAMETSVEGVFAAGDVR
                     DKGLRQVITAASDGAIAAMSASSYINEQVHLRSVLIEPAHVVALFYSSIDREQVKLSD
                     EVEALSQQSGKKVALIDGYKNHRMVEKFGLGSL"
BASE COUNT         1017 a         1342 c         1492 g          968 t
ORIGIN      
        1 cagaagaagt acgagggaat caactacggc cagagagaca acgcggaata agacgacgac
       61 gagggggctt cggtcccctc tttttcggaa aggaagtggg ctgtatgcgc tgcggcgtcc
      121 tccttctctc cttcacgccc cggcctgatg ctgttgtcgc cgctgcggca aaactctgtt
      181 acagcgacgt ttcggctgcg gaccttctct cggatgaatc ttcggagaag atgcgcggtc
      241 tgttgcgtca tatccgcgca agcggtcatt tctcaccgtt cgagcacgcc tccttcacct
      301 ttgccgtgga cggcttgagc agggtaacgt ctcatcagct tgtccgccac agaatagctt
      361 cctttagcca gcagagccaa cggtacgtga cgatgggaga gcccgatgtc gttgtacccc
      421 cttccattgc ggaacgcccc gaactcacgg acggttttct cgaaatgacc agatcggcgc
      481 acgctttcta ccggaagctc gtcgaaagcg gcgttccgaa ggaggatgcc cgctacattt
      541 tgccccatgg ctggagcaca cggcttgtca tgacgatgaa cgcgcgggag ctccatcatt
      601 ttttcacact ccggctctgc cgcagggcgc agtgggagat tcggaatctg gcgcgggaga
      661 tgcttctcgc ggtgcgtgag gcggcgcccc tgctcttcga gacggcgggg ccgtcgtgcc
      721 tggttcgcgg acgctgcgag gagaagtctc cgtgcggccg cccgtttgcg tctcttgagg
      781 aacttttgga tgataattaa gtgtgaggtg aacatgaaga gattatttgc gtcgatgctc
      841 ctcttccttg cggaagggct tgagcagaag agaataccgg tcggcggcca ggcggtcatc
      901 gaaggcgttc ttatgaaggg tccggagcgc tggggacttt cggtcagaaa accggacgga
      961 gagatcgaga acgagacgtg gcaaagcttc tcgcggacga agcggatgcc gtggaagctt
     1021 ccgatagtgc gcggcgtcgt gacgatggtg gagatgatga cggtcggttt ccgcgcgctg
     1081 aacaagtccg cgcagatcgc cgtcggtgaa gaagaaaaaa taacgccgaa ggagtttctc
     1141 ctctcggtcc ttgtcgccat tggggcggtc gtcggactgt tcatcgcctt gccgctttgg
     1201 ctctccgatc ttgccgttac ttcgtggggg ctgacgcaaa ccggaaaaaa cattctcgaa
     1261 ggatgtgtca ggggccttgt ctttatcgcc tacgtagggg ggatcggcct ctggagcgag
     1321 atacggcaag ttttccgcta ccacggggcg gagcacaaga cgatcaacgc ctttgagtcc
     1381 ggggccgaca tgacgcccga gaacatacgg aaattttcgc ggattcatcc gcggtgcggc
     1441 acatcgttcc tcctcatcgt cgttgcggtg agcatcgtgg tcttctccat cgcgggaagc
     1501 ggctccatcc tctggagaat cgacagcagg atcgtgcttc ttccgctggt catgggaatt
     1561 tcctacgaga tcatcaaagg agcctcttgc gccggtattc tgggcaggat cctcatggct
     1621 ccggcaatgt ccttccagta tctcacgacg cgggagccgg acgagaagca gatcgaagtc
     1681 gccctgacgt cgcttgagac ggcgcttggc cgtaagctct cgcctgttgc ggcggaggcg
     1741 cctgctgaat gaacttccaa gacaagctga gcgagctcga aagggatttt gaagaaacag
     1801 aagccaagat ggccgaccct gcctttcact cgaatatgaa ggagctgcaa ggactggcga
     1861 agcgccattc gaagctcgct cccgttgtct ccaagttccg cgagtttaaa aaagtaaccg
     1921 ttgacctcga agaggcgcgg tcgctgcttc actgcggcga tccggagatg gagtcgctcg
     1981 ccaaggatga actctcctca aaagaagatc gtctccggga gatcgaagag gagatgaaac
     2041 tccttcttct gcccgaggac ccgaacgacg aaaagagcgt tattgtggaa atccgcgccg
     2101 ggacgggggg ggaggaggcc gcgctctttg ccgcaaacct cttccggatg tacacacgct
     2161 tcgcggagcg cgaggggtgg agaaccgaga tcgtcgacta caacgagacc ggtatcggcg
     2221 gatacaagga gattatcttc cggatcgacg gcgacggcgt gtacagcaag ctcaagttcg
     2281 agagcggcgt gcacagggtg cagcgcgttc ccgcgacaga ggccgccgga aggatacata
     2341 cctccgcggc gacagtcgcg gtgcttcccg aggtggagga tgtcgacgtg gagatccgga
     2401 ccgaggatct gaaaatagac acctaccgtt cgagcggcgc gggcggccag tacgtcaaca
     2461 tgaccgactc cgcagtaagg attacgcacc tcccgacggg cgtcgtggcc acctgccagg
     2521 acgaacgctc acagctgaag aacagggtaa aggcgatggc ctaccttcgc gccaagctgt
     2581 acgacctcga gctgcagaag caaaacgacg aagtagcctc cgaacgcagg gggcagatag
     2641 gctcggggga ccgttcggag cgtattcgga cctacaattt ccctcagaac agaatctccg
     2701 atcatcgcat cgggatgacc ctctacaagc tggaccagta tctggacggc gatctttacg
     2761 aactcgtcga cgccatgaca atggccgacc agtcgcagaa actccacgct atcgaggcga
     2821 actagcatga ccgcaaccga ggccaggaga aggctcgtcc gcgaactttc agcggcggga
     2881 gtcttttctc ccggcctcga agcggatgtt atcctttcag ttgcgtgcgg cgtttcgtcc
     2941 gcgcttcttc gtgcccgacc cgatttcgag ctgaccgaag aaaacgtttc ggaactcgcc
     3001 cgcatcaccg gaaggcgcgc tcgaagggaa cctctccagt atattctcgg atcatgggaa
     3061 ttttacggcc gcgcccttga gaccccgccg ggtgtcctga ttccgaggcc ggagacggaa
     3121 gtgctcgttg agaaagccct ggagcgcctt ccacccgagg gaggcgtctt tctcgactgg
     3181 gggacgggga gcgggtgcat cgcgctttcc ctcctctgcg aaaacgcttc cgcctgcgct
     3241 atagccgccg attgcaaccc tcgcgcgctt cgagtatcgt ggacgaacat gaagcgctgg
     3301 ggcgttctcc ggaggtgcct cctgtggcat tcgcgcactc ccgacgatct tcccgtctct
     3361 cccgcatccc tcgatatgct cgtaagcaac ccgccctata tcccgacgga ccgtatgaac
     3421 acgctcatgg aggaggttcg gtacgaaccg caatccgcct tggacggcgg tatagacgga
     3481 gcggactgtt acaggcggct tttcatcgca ggagaacgga tgctgcgccg cggagggagt
     3541 cttctcttcg aggcgggcga cggagaacag attgcttttt tagcgggcat tgctccagag
     3601 tgctttgaat tggatggaat ttttgaggat ttttcgaagt cgcctcgcgt aattgcatgg
     3661 cgtcgtgtat aatttcgaaa agagatcatg tgtcttatct ggagggatag tttcgatatg
     3721 gaaaaaagag agcttgtaat catcggtgca gggccggccg gtttatcggc cgcgatctac
     3781 ggcagaaggg ccggacttga cgtgcttctt ctcgaaaagg gcgtgcccgg cggacagatc
     3841 aacatcaccg aggaaattga gaactggcca ggagtggagc atgccaccgg tccggagctt
     3901 ggcaggatgt tcagaagcca cgccgagaag ttcaagaccg aattccgcga tgtcgacgtt
     3961 tccaaggtcg aaattcgcgg cgacgccaag atcgtcgtca caaacaaggg cgagattgaa
     4021 gccgaagcgg ttatcatcgc caccggggcc tatttccgca ggctcgggtg cgagggcgaa
     4081 gccgaacata tcggccaggg cgtgagctac tgcgcagtct gcgacggcgc cttctttgaa
     4141 ggcgaggaga tcgcggtcgt cggcggcggc aacacggccg tcgaagaggg cgtctacctc
     4201 acgaacttcg cttcgaaagt cttcatcatc cacagaagag acgagttccg cgccgataga
     4261 gcggcagtcg aacatgccat gtcgaatccg aagattgtcc cggtgtggaa caccgttgtc
     4321 gaaaagatcg aaggcgacgg catggtcgag aatctggtcc tgaagaacct caagaccggc
     4381 gaggtctcca accttcctgt ggcgggcgtc ttcatgttcg tcggccagtc tccccacgac
     4441 gagtgtatcc gggggcttgt tgacgcgtcg aaaggcggct ggatcaagac gaacgacgcc
     4501 atggaaactt ccgtcgaagg cgtcttcgcg gctggagacg tccgggacaa ggggcttcgc
     4561 caggtgatta cagcggcctc cgacggcgca atcgcggcaa tgagcgcttc ttcgtatatc
     4621 aacgaacagg tccacttgcg ctcggtcctc atcgagcccg cgcatgtggt cgcgctcttc
     4681 tactccagca tcgacaggga gcaggtcaag ctctccgacg aggtcgaggc gctctcgcag
     4741 cagagcggga agaaggttgc tcttatcgac gggtacaaaa atcaccggat ggtggaaaag
     4801 ttcggcctcg gttctcttc
//
LOCUS       DAOSWW010000080         4809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_11938_length_4809_cov_3.737484, whole genome shotgun sequence.
ACCESSION   DAOSWW010000080 DAOSWW010000000
VERSION     DAOSWW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11938_length_4809_cov_3.737484"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK442_02645"
     CDS             <1..387
                     /locus_tag="PK442_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOO86578.1"
                     /translation="TMKNKIDVERYISEDAGLPTLTDILNELEKPGRDPRETFEPFTF
                     QEGVEKIDDLLPGMPLPGIVTNITSFGIFVDVGVHQDGLVHRKRLPHGKEFRPGERVN
                     VSVLEVDRPRKRISLSMREEKKGTNR"
     gene            384..1637
                     /locus_tag="PK442_02650"
     CDS             384..1637
                     /locus_tag="PK442_02650"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO86579.1"
                     /translation="MSAPQIVVIGAGPAGLIAAGRAAEKGARVILVEKNQTVGEKLLI
                     SGKRRCNLTNAEEDMDVFLSKYGPNGKFLYSSFAGFGPRETVSFFQSRGVPLKEERGK
                     RFFPATGGSEGVLAALLNYVREGGVTIYRKRESINLEIRDGRVRRFILRGQEVEGDAF
                     IICTGGKSFAKTGSTGDGYRFARRAGHTVIPPVPALCPVRTVEKWPLASRGMTLRNVS
                     LTLWKNDEVASQRFGELLLTHFGISGPIAMDMSKEIAAAMETGTARLTLDLKPALSLP
                     VLKNRIGRDFEKFSGRKFKDSLGELLPRGLIPAVLALKPLPEDKIVNSASNQEIETLA
                     ELLKAFPLTPDGLLGFDWAIVTSGGVALKEIEPRTMRSKLVENLFFAGEVLDLDGPTG
                     GFNLQMCWSTGYQAGESAADLSLPR"
     gene            2625..4310
                     /locus_tag="PK442_02655"
     CDS             2625..4310
                     /locus_tag="PK442_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOO86580.1"
                     /translation="MREFSIPRSALPPGSIILGDPFYNIQEYTPFIFANLFLLLFLAG
                     LAVYLKRKINRRKKTEEELLRQTEYWKKLFEHSPQGILIYDGAGQVKEANSRFREMFR
                     LSGPAPLPENITSLLSGTEADFSRNDIFPQEENISGTAAPAKETALPDGDGGTLSVSF
                     QAFPLSSNDEETHYCALFHDIRDRKTMEQKLHRRTILQERMTAISSRFVLFQNFRDSM
                     EAALEDILALSGARTASLFVISDSGILIPEMEAFSPDKAGPSLAANFSDGDDFFWKSL
                     LLNDALPSVITIESAVHKVRESKWDFLKEMGIDSITILPLFVKTSFQGFLALTDPLQQ
                     WVDMSNDPLLGLFSDLMAMAIERRKEEDSLRETHERIHDRFTGAITALCQVSELRDVS
                     TSGHQKNVSVLAEDLANEMQLPEEVRMGVRYAGLVHDLGKLYIPAEILGKPSRLSEAE
                     YELVKKHPEFGHDILSLLNFPWPLAEIVLQHHERVDGTGYPFSLKRKDIRIEARILSV
                     ADAFEAMTSDRPYRKRKTAKAALKELMELAGKAYDAKIVKSFVKMILERGENP"
     gene            complement(4418..>4809)
                     /locus_tag="PK442_02660"
     CDS             complement(4418..>4809)
                     /locus_tag="PK442_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOO86581.1"
                     /translation="VPATTGAAKAMVPLFPELKGKMDMISVRVPVADGSLTDIVVHFK
                     KEVTVESVNAALKSAADGKLKGIVEYNDEEIVSADIIGNTHSGIVDAPSTKVIMGKVA
                     KVMVWYDNEYGYSNRMVELAQYIAGKE"
BASE COUNT         1172 a         1307 c         1229 g         1101 t
ORIGIN      
        1 acgatgaaga acaaaattga tgtagagcgg tacatctccg aagacgcggg ccttcctacg
       61 cttaccgaca tactgaacga actggaaaaa cccgggcgcg acccgcggga gacattcgag
      121 ccgttcacat ttcaagaggg cgtcgagaag attgacgacc tgctccccgg catgccgctt
      181 cccgggatcg ttacaaacat cacttctttc ggtatctttg tcgacgtcgg cgtacaccag
      241 gacggactcg tccacaggaa gcgcctgccg cacgggaaag agtttcgtcc cggagagcgc
      301 gtcaacgtct cggtacttga ggtggatcgt ccccgcaaac gtatctcgct ctccatgcgt
      361 gaagagaaaa agggtacgaa ccgatgagcg ctccccagat cgtggtaatc ggcgccggtc
      421 ccgccggact gattgccgcg ggacgcgcgg ctgagaaagg cgccagggtc attctggtcg
      481 agaaaaatca gaccgtcggt gaaaagctcc ttatcagcgg gaaaagacgg tgcaacctga
      541 caaacgccga ggaagacatg gatgtctttc tctcaaagta cggaccgaac ggaaaatttc
      601 tctactcctc tttcgccggc ttcggacccc gcgagacagt ttcttttttc caatccaggg
      661 gagtgccgct caaggaagaa cggggcaaac ggttcttccc ggcaacaggc ggatcggagg
      721 gggtgctcgc agcccttctg aactatgtac gggaaggcgg agtcaccatc taccgcaagc
      781 gcgaatcgat caacctcgag attcgcgacg gtcgggtccg tcgcttcata ctcaggggac
      841 aagaagtgga gggagacgcg ttcatcatct gtacgggggg aaagtccttt gcgaagactg
      901 gatcaacagg cgacggttac cgcttcgcac gcagggcggg acacaccgtc attcctcctg
      961 ttccggcgct ctgcccggtc agaacagtgg aaaagtggcc ccttgcttcc cgggggatga
     1021 cactacgtaa cgtctcgctc actctttgga aaaatgatga agtggcatcg caacgctttg
     1081 gagagcttct gctgacacat ttcggcatca gcggtccgat tgctatggac atgagcaagg
     1141 agattgcagc cgccatggaa acgggcacgg ccaggcttac tctggacctc aagccggcgc
     1201 tttctcttcc tgtgctgaaa aacaggattg gcagggattt tgagaagttc tccggcagaa
     1261 aattcaagga ctctctcggg gagcttctcc caagggggct cattcctgca gtactcgctc
     1321 tgaagcctct accggaggat aaaattgtga actccgcaag caatcaggaa atcgaaacgc
     1381 ttgccgagct gctcaaagcc tttcctctga ctccggacgg acttctcgga ttcgactggg
     1441 caatagtgac cagcggcggt gtcgcgttga aggagatcga gccccgaacg atgcgatcca
     1501 agctggtgga gaacctcttt ttcgctggag aagtgctcga tctcgacggt cctaccggcg
     1561 ggttcaacct ccaaatgtgc tggagcacag ggtaccaggc aggagagagc gcggctgacc
     1621 tctcattgcc gcgctaaaca aaccgagaga ggagatacat agtgccgatc aacatttctt
     1681 tcctacgaaa gcaattccgg tatctcgtac tcgttgcagc gcttcttctc cacggcacac
     1741 tttcatcgtg cgcggctccc agggtagccg gaaagattct actgctcaac tcatactcca
     1801 ttgaagacac gtggactgcc gagacaaatc gaggcatcgc agcagctttt gaggagaacg
     1861 gagccaatgt cgaattcttt gttgaaaacc tcgatatcac ggggacgttc tccgcaatgc
     1921 acctcccgga actccgggag ctctatcgta ctcggtacac ttcaatgcgc ccggatctta
     1981 tcatcacaac ggaaaatgac gctacatcat tcgctctcgg acaaagaggc gtccttttcc
     2041 ccgacgttcc catcatcttc tgctccctta cgaacaaagc gcttctctct attatccccc
     2101 ggacaaaggg aacgggaatc ttcagtgaaa acacctcgct ttttatattg cgggaaataa
     2161 tccgccttca tcagaacgcc ccccgtatcg cgcttgttac ggatatgtcg cctcgcgggt
     2221 ttcgtgatat tgcacaaatg aacgaggcgg caacaatgct ctcgattccg agaaaacgtc
     2281 tgcaaccgct tttcggaatg gatatcgaac agctcacaac atctctttcc gagcttcctc
     2341 cgaattctgt tatcatcctc gggcactaca atattacccg ggagggagat ctcgtttctt
     2401 taccccgtct gatagaggct attcaagaag cttcggatct ccctgtgtac tctctcatag
     2461 aagaaggggc cgcattaggc gttttggcca gcgtctccga aggaggcttt cagaaggggt
     2521 tcgacgcggg agaaattgcg tttgagctat taaaaggatc ttctcccgaa tcgatgcctc
     2581 cgagagaagg acaggctccg aaacttcttt ttaactactc cggaatgcgg gagtttagca
     2641 tcccaagatc ggcccttccc ccgggaagta ttattctagg agatcccttt tataatatcc
     2701 aggagtatac tccttttatt ttcgcaaacc tttttctcct tcttttcctt gcgggattgg
     2761 ccgtttatct gaagagaaag atcaacagac gaaaaaaaac ggaagaggag ctccttcgac
     2821 agacagagta ctggaaaaag ctctttgaac actctcctca aggtatcctt atctacgatg
     2881 gcgcaggcca agtaaaagag gcaaacagcc gtttccggga aatgttccgt ctttcggggc
     2941 cggctccgct tccagaaaat atcacgtcgc ttctctccgg aaccgaagct gatttttcgc
     3001 gaaacgacat ttttccacag gaggagaaca tttcaggtac agccgcgccg gcaaaggaga
     3061 cggctcttcc tgacggggac ggcggaacgc tctctgtctc attccaagcc tttcccctgt
     3121 cgtcaaatga tgaggaaact cattattgcg ctctcttcca tgatataaga gacaggaaaa
     3181 cgatggaaca gaagctccat cggagaacga ttcttcaaga gcgaatgaca gctatttcct
     3241 ctcgattcgt cctcttccag aacttcaggg actccatgga agccgcgctc gaagatatac
     3301 ttgcactttc gggagcgcgt accgcctcgc ttttcgtgat ttccgattca ggaattctga
     3361 ttcctgaaat ggaagcgttt tctccggata aagcgggccc ctctcttgcc gcaaacttct
     3421 ccgacggcga cgatttcttc tggaaatctc tcctattgaa cgacgccctt ccctccgtca
     3481 tcaccatcga atcagcagta cataaagtaa gagagagtaa atgggatttt ctaaaggaaa
     3541 tggggatcga ctccatcacg attctccctc ttttcgtcaa gacaagtttt caaggatttc
     3601 tagcgctcac agaccctctc cagcagtggg tagacatgtc caatgaccct ctgctcggtc
     3661 ttttctccga cctgatggca atggctatcg agcgacgcaa agaggaggac tcccttcggg
     3721 agacgcatga gcgaattcac gacagattca ccggagccat aacagcgctc tgccaggtca
     3781 gcgaactcag ggacgtctcc acatcgggac atcagaaaaa tgtttctgtt cttgcagagg
     3841 accttgcaaa cgaaatgcag ctccccgaag aggttcggat gggggtcagg tacgcaggac
     3901 tcgtgcatga tttaggtaaa ctctatatac ctgccgaaat cctgggaaag ccctcccggc
     3961 tctcggaagc cgagtacgag ctcgtaaaaa aacaccccga attcggacac gatattctct
     4021 ccctgctgaa tttcccctgg ccattggcgg agattgttct tcaacaccat gaacgcgttg
     4081 atggaacggg ctacccgttt tccctgaaga gaaaagatat tcgcattgag gcgcgtatct
     4141 tgtctgtcgc agatgctttt gaggccatga catccgatcg cccctacaga aagagaaaga
     4201 cggcgaaagc cgctctgaag gaacttatgg agcttgcggg gaaagcctat gacgcaaaga
     4261 tagtgaagtc gtttgtgaag atgattcttg agcgtgggga aaatccttaa gagtacaagc
     4321 aactgggaca gcgcgcactg aatctttgta aaaacgcaag cgcccgagct cccatcgggg
     4381 gactcgggcg cttgcaaaac gaactgtgaa agatgggtta ttccttgccc gcgatgtact
     4441 gcgccaactc caccatcctg ttcgaatagc cgtactcgtt gtcgtaccac accatgacct
     4501 tggctacctt gcccatgatc accttggtgg agggcgcgtc gacaataccc gagtgcgtgt
     4561 tgccgatgat atcggcggag acgatctctt cgtcgttgta ctcgacgatt cccttcagtt
     4621 ttccgtcggc tgccgatttc agcgccgcgt tgactgactc tacggtaact tcctttttga
     4681 aatgaacgac gatatctgtc agagatccgt cggccacagg aacacgtaca gagatcatat
     4741 ccatcttgcc cttgagttca gggaaaagag gaaccattgc ttttgccgca cccgttgttg
     4801 caggcacaa
//
LOCUS       DAOSWW010000081         4773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_12062_length_4773_cov_3.090716, whole genome shotgun sequence.
ACCESSION   DAOSWW010000081 DAOSWW010000000
VERSION     DAOSWW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12062_length_4773_cov_3.090716"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="PK442_02665"
     CDS             complement(<1..1278)
                     /locus_tag="PK442_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO86582.1"
                     /translation="MNSLCCAEGATEVDLLRALLQEGGISPVFQPIVDTATGSVYGYE
                     VLSRGKAPLESPPSFFSVARANNLLWETERACRKKALATIRDSEEASALRFFIKVSPG
                     VFTDPRFRDVFTPEALKPYGIAPERVVLEITESIPVADYSELEKVVACFRERGFSIAI
                     DDLGAGHSGLRTLSICAPDFLKLDMALVQGISMDPYKKHVVAFLCSFASAVGGKIIAE
                     GVETWEDMKTLLELGAPLAQGFLFARPAPGFQRPAPFVLERLALLRRAVCKGDCIVGE
                     GLRSLVIRRTTAETGEMTCEELERIFRKNRTLDHLVVLENEAPVGLVTRQNFFLKTGG
                     TFGYQLFQKRPLEDIATASFLKAPVFSSVSTLAKLAMEREPEELYDPVVVVDDCGLFL
                     GTVTMKQIIARAGELEVERAMSCNPLSGLPGNRH"
     gene            1507..3384
                     /gene="typA"
                     /locus_tag="PK442_02670"
     CDS             1507..3384
                     /gene="typA"
                     /locus_tag="PK442_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOO86583.1"
                     /translation="MRRAEHIRNIAIIAHIDHGKTTLIDSIFKAAQVFRDNARVEERF
                     MDNNELEREKGITIRAKHCTVEWKGYRINIIDTPGHADFSGEVERILSTVDSVLLLVD
                     AGEGPMPQTRYVLSHALRMGIKPIVYINKVDRKEADPVLALNATFDLFFELGATDEQA
                     DFPVLYGSGLAGWAVKELSEIEGGESKGMDALFETVIEYVEPPLADEESPFLLQVSTL
                     AWNEYTGRMGCGKILQGKIRKGEEFLRTTTAWEDRADKENENFVIAGKDRTKAVQIFV
                     TRGLERLEVEEAGAGDIVWISGPKEIGIGDTFCSPELENFPLPPLDIEEPTVSMFFLV
                     NNGPFSGQEGQAITLRQLKARLEREMHVNVALRMEDLGRPDGVKVSGRGELQLAILIE
                     EMRREGMEFCVSKPEVITEERDGKTFEPMEDLVVDIPEEYQGVVFEKLSRRKARVQEI
                     QNLRTGLMRIFFAVPTRGLIGYRGEFLTDTRGLGIMSSRFSGYAPWVGEITPRNRGAL
                     VSLDTGEATSYQLENLQERGVLFISPMDKIYSGMIVGENARPGDMPCNPTKRKQATNH
                     RSSTKDFTIKLDVPRKMTLEKALEWISEDELVEVTPKSIRLRKSILDDNDRRKARRIS
                     A"
     gene            complement(3426..3842)
                     /locus_tag="PK442_02675"
     CDS             complement(3426..3842)
                     /locus_tag="PK442_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86584.1"
                     /translation="MKEGLHTPLSRLASQVRSFSGCDVRFSIEGRADVLSLQVRTAVF
                     KIVQQAVMNAVRGGKAKNVKIMAGIGGDALRVKVLDDGIGFDVAKERELAEERGTWGL
                     VNMEERARLIGGSLSIASEPGRGTTVLLEMPLTKKV"
     gene            complement(3757..4554)
                     /locus_tag="PK442_02680"
     CDS             complement(3757..4554)
                     /locus_tag="PK442_02680"
                     /inference="COORDINATES: protein motif:HMM:NF017221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86585.1"
                     /translation="MDELSRKLKEIQDKMSEALHYSIDSISSLREEERERLFEVRTRK
                     EDLIRELNEVIVAADKAESEYRRSREELLVASRSGNEKREKDAYERAMHLMKIRGAFE
                     EREKLLARQRDDLAREERRIEKLLVRSEEMGNRFRVALNLLSMNLEGESPASSDENGL
                     LAGMLLAERESRLAFAGSPRRPYSEVFQHRADDRPLQRVSRQGRFCPCGGGAGTDEGA
                     PGGRSSRSAFFSLPAQPLGAERGPAHPSEPPCFSGTILLGVRRSFFD"
BASE COUNT         1041 a         1495 c         1313 g          924 t
ORIGIN      
        1 atgccggttg cccggcagtc cgctcaacgg attgcagctc atcgcccgct caacctccag
       61 ctcccccgcc ctggcgatga tctgcttcat cgtaaccgtt cccagaaaga gtccacagtc
      121 gtccacgaca accacgggat cgtacagctc ttcgggctcc ctctccatgg cgagcttcgc
      181 caatgtgctc acggagctga aaacaggggc ctttaaaaag ctcgccgtcg cgatatcctc
      241 caggggacgc ttttgaaaaa gctggtaccc gaaggttcct cccgtcttca ggaaaaaatt
      301 ctgtctggtg acgaggccga cgggagcttc gttttccaga accaccagat gatcgagcgt
      361 cctgttcttt ctgaagatgc gctcgagctc ctcgcaggtc atctctcccg tttccgccgt
      421 tgtacgccga atcactagcg aacgaagtcc ttctcccacg atgcagtccc ccttgcagac
      481 agctctccgc aacaacgcca gacgctccag cacgaacggc gcggggcgct ggaacccggg
      541 cgccgggcgt gcgaacaaaa agccttgggc gagaggcgct ccaagctcca gaagcgtctt
      601 catatcctcc catgtttcca ccccctccgc gatgatcttg ccgcccactg ccgaggcgaa
      661 ggaacacagg aacgcaacga catgtttctt gtacggatcc atcgaaattc cctgcacaag
      721 cgccatatcg agtttcaaaa aatcgggagc gcagatgctg agcgtccgca accccgaatg
      781 gcccgcgccg aggtcgtcga tggcgatgga aaaacctcgc tcgcggaaac aggcgacaac
      841 tttctcaagc tcgctgtaat ccgcgacggg gatgctttca gtgatctcaa gaacaacccg
      901 ctccggcgcg ataccgtagg gcttgagcgc ctccggggtg aatacatctc tgaaacgggg
      961 atcggtaaag accccgggac tgactttgat gaaaaaacgc aacgccgacg cttcttcgga
     1021 atcccgaatg gtcgccaaag ctttttttct gcacgcgcgc tccgtctccc agagcaggtt
     1081 gttcgcccgc gccaccgaaa aaaaagaggg gggcgactca agaggcgcct tcccgcgcga
     1141 aagcacctcg tagccgtaca cgctccccgt agccgtatcc acaataggtt ggaagaccgg
     1201 cgagatcccc ccctcttgaa ggagggctcg caacagatcg acttcggttg ccccttccgc
     1261 gcagcacaat gaattcataa acgtccgcct cccggcttga aaatcaactt ttttaccaac
     1321 agacgatagc atcgaagcgt tacaatggtg tggcgttccg gcaaaattta tgtaacactg
     1381 cgcgcggaga ggttaaaatt cagatttccg ccgaatactc ttgcatttga cgcctccagg
     1441 ttgtatcatg ctttccctgc ggttgaaata atcaagccag gaccgcattc ggaggaacga
     1501 agtgtaatga gaagagccga acacatacgc aatattgcaa taatagccca tatcgaccac
     1561 ggcaagacta cccttatcga ttccatcttc aaagccgccc aggtattccg cgataacgca
     1621 cgcgtcgaag aacgctttat ggacaataac gagctggaac gcgagaaggg aatcaccatc
     1681 cgcgccaaac actgtaccgt cgagtggaag gggtacagaa tcaacatcat cgatactccg
     1741 ggacacgcgg acttttccgg cgaagtggaa cgcatcctct ccacggtcga ctccgtgctg
     1801 ctgctcgtgg acgcgggaga ggggccgatg ccccagaccc ggtacgtcct ctcgcacgcg
     1861 ctccggatgg ggatcaagcc gatcgtctat atcaacaagg tggaccgcaa agaggcagac
     1921 ccggtgcttg cgctcaacgc cacgttcgac ctctttttcg aactcggcgc gacggatgag
     1981 caggcggatt tccccgtcct ctacgggtcg ggacttgccg gttgggccgt caaggaactg
     2041 agcgagatcg agggcggcga atccaaaggg atggatgccc tcttcgaaac cgtcattgag
     2101 tacgtggagc ccccgctcgc cgacgaggag tctcctttcc tgttgcaggt gagcacgctc
     2161 gcctggaacg aatacaccgg acgcatgggg tgcgggaaga tcctccaggg gaagatacgc
     2221 aagggggaag aattcctccg cacaacgacc gcctgggagg acagagccga caaggagaac
     2281 gagaacttcg tcatcgccgg aaaagaccgg acaaaagccg tccagatctt cgtcacgcgc
     2341 ggtcttgagc gtcttgaagt ggaagaggcg ggagcgggcg atatcgtctg gatctccggc
     2401 cccaaggaga tcggcatcgg cgatacgttc tgctcgccgg agcttgagaa ctttccgctg
     2461 cccccgcttg atatagagga gccgacggtc tcgatgttct tccttgtgaa taacggcccc
     2521 ttctcggggc aggaaggaca ggccatcacg ctccgtcagc tcaaggcgcg gctggagcgc
     2581 gaaatgcacg tcaacgtcgc cctccgcatg gaagacctgg gacgtcccga cggagtcaag
     2641 gtctccggac gcggggaact ccagctcgcg atcctcatcg aggagatgcg ccgcgagggg
     2701 atggagttct gcgtctccaa accggaggtc atcaccgagg agcgcgacgg caaaaccttc
     2761 gagccgatgg aggatcttgt cgtggacatc cccgaggagt accagggcgt cgtcttcgag
     2821 aagctgtcgc gacgcaaggc gcgcgtgcag gagatccaga atctccgtac gggactgatg
     2881 cgcatcttct tcgccgtccc cacccgcggc ctcatcggct accgggggga attcctgacc
     2941 gacacgagag ggcttgggat catgtcgtcc cggttctccg ggtacgcccc gtgggtaggc
     3001 gagatcacgc cgcgcaacag gggcgcgctg gtcagcctgg acaccggcga ggccacaagc
     3061 taccagcttg aaaatctcca ggagcggggc gtcctcttca tctcgccgat ggacaaaatc
     3121 tactcgggaa tgatcgtggg ggaaaacgcg cgcccgggcg acatgccctg caacccgacg
     3181 aagcgcaagc aggcgaccaa ccacagatcc tccacgaagg actttactat aaagctcgac
     3241 gtcccgagga aaatgaccct cgaaaaagcc cttgaatgga tctccgagga cgaactcgtc
     3301 gaggtgacgc ccaagtcgat ccgtcttcgc aaatccatcc tggacgacaa cgatcgaaga
     3361 aaggcgcgac gcatcagcgc gtaaagtaaa aagcgccgct cctcagacgg ggagcggcgc
     3421 tttttttaca ctttcttcgt cagcggcatt tcaagaagaa cggtcgtccc tctgcccggt
     3481 tcgctggcaa tagagaggct cccaccgatc aggcgagcgc gctcctccat gttgacaagc
     3541 ccccacgtgc cacgttcctc ggccagttcg cgctccttcg ccacatcaaa accgatgccg
     3601 tcgtcaagca cctttacacg aagggcatcc ccgccgatac cggccattat cttcacattc
     3661 ttcgcttttc cgccccggac ggcgttcatc accgcttgct ggacgatctt gaagaccgct
     3721 gtacggacct ggagcgacag aacatccgca cgtccttcaa tcgaaaaacg aacgtcgcac
     3781 cccgagaagg atcgtacctg agaagcaagg cggctcagag gggtgtgcag gccctctttc
     3841 agccccgagg ggttgagctg gaagagaaaa gaacgcgctt cgagaagagc gtccgccagg
     3901 tgcgccctcg tccgtcccag ctcctccccc gcacggacaa aatctcccct gtcgagatac
     3961 tctttggaga ggtcgatcat cagcccgatg ctggaaaact tctgaatagg gccgtcgtgg
     4021 agatcccgcg aaagcgagac ggctctcccg ctccgcaagc aacattcccg caagaaggcc
     4081 gttctcgtcg gaggaagccg ggctctcccc ctccaggttc atgctcagca ggttcagcgc
     4141 aacccggaag cgattcccca tctcctcgct ccgcacaagc agcttctcga tgcggcgctc
     4201 ctcccgggcg agatcgtccc tctggcgggc gagcagcttc tctcgctcct cgaaagctcc
     4261 ccgtatcttc atcaggtgca ttgcgcgctc gtaggcgtct ttctcgcgct tttcattccc
     4321 ggaacggctg gctacgagaa gctcctcccg tgaccggcga tattcgctct ccgccttgtc
     4381 ggcggcgacg atcacctcgt tgagctccct gataagatcc tccttgcgcg tgcggacctc
     4441 gaagagacgc tcccgttcct cctcgcggag cgacgagatg ctgtcgatac tatagtgcag
     4501 cgcctcgctc atcttgtcct gtatttcttt cagcttccgt gacagttcgt ccaagggcaa
     4561 gcgcggcgcc tccttttttc tctttgaacc cggtacgcgc atcgtttctc atatatcgct
     4621 tattgtaccg ttgaaacgga ggtctggcaa ttttcgccgg aacggataca atgaagtgac
     4681 tttctcatcg aaggagatgc atttcatgat cctcgtgctc attcttggct gggcggtgct
     4741 ctacgccgac cgtacggccc tctaccccct gct
//
LOCUS       DAOSWW010000082         4771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_12069_length_4771_cov_2.954835, whole genome shotgun sequence.
ACCESSION   DAOSWW010000082 DAOSWW010000000
VERSION     DAOSWW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12069_length_4771_cov_2.954835"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK442_02685"
     CDS             <1..430
                     /locus_tag="PK442_02685"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOO86586.1"
                     /translation="DEGNKLREKGAEFGATTGRPRRCGWLDLVALRYAVRVNGISSIA
                     LTKLDVLTGQERIPVCTSYMVEGQDEPHFPCALQRLEKASPHYEYLEGWHEDIASCTS
                     YEQLPPQARKYVEHIESATGVPVNLIGVGPGRDQTILRNF"
     gene            506..904
                     /locus_tag="PK442_02690"
     CDS             506..904
                     /locus_tag="PK442_02690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO86587.1"
                     /translation="MEERSSVAPWPRTLIRRDLAGDTPMLYLGAWGKDILLGFAALGP
                     CEKGTELTNIAVLPEYRRKGIGSQLVLGVSEVALSLGYSRIYLHVRVSHEESRTFYGG
                     FDFSPLYVVPGYYLDGEDALFMEALLPLSV"
     gene            complement(928..1731)
                     /locus_tag="PK442_02695"
     CDS             complement(928..1731)
                     /locus_tag="PK442_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO86588.1"
                     /translation="MLTRILFFADVSSFSERALAWAARQVAGDRSDFLILHVVDRAAG
                     LDAPELVREAEGYLEEMCARTLPPESYYKTLVLLGDLLESLPDTIRSEKCTFAIFPLP
                     GGEDGMPLVRATPAPQILLREHEGFFPEEDTFGSLAVALDLEPSRTSLMLDNLREFLA
                     QARKNPKITLLHALAPQSAEDAPEVLNAGRQALEEVRDEISSWGYETSAEIVSGDPSK
                     ELPARIRELAPSLLVIGLPVAGELGELVFGSTAEMLFAGTTCPLLVFPL"
     gene            1856..2671
                     /locus_tag="PK442_02700"
     CDS             1856..2671
                     /locus_tag="PK442_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO86589.1"
                     /translation="MPLTWSVRFGASLGWLLWALDKKKVDEAERRCVRALQVGPTIAR
                     GIVRRSYMNLGRSVAEFAAVPGSGRSMRSIVHVHGEEHLREALDSGNGVIFFSGHIGN
                     WEFGAAVLAEMGYPMNAIGADQRDERVTDLIIGLRAVSGVTTVSKGFDLKAAIRCLKR
                     GEALAILIDQDVRERGVVVPFLGIPASTPYGPAKIAGKIGSVILPSFMLRRGTSEEHD
                     LHILPSPWKNGRPERDESMEEAMARCNDLISEYIRRAPDQWMWLYPRWASTGE"
     gene            2650..3129
                     /locus_tag="PK442_02705"
     CDS             2650..3129
                     /locus_tag="PK442_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOO86590.1"
                     /translation="MGVDGRVAMFLGIDPGRDKVGWALTDAEGELLLSGIFRAGDAAL
                     FFSAFESGALERLAPFILEKTSGEIADFASAELLMGDGTGKEYLLSLAQSASLGVKLV
                     SERETTLLARKLYWKLHPPRGVWRLLSAGLRVPPRDIDDLAAWAIVLRYCGVQSKEK"
     gene            3150..3635
                     /locus_tag="PK442_02710"
     CDS             3150..3635
                     /locus_tag="PK442_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOO86591.1"
                     /translation="MGTEKLATLVNTFGSSLMTVSKEMGVELTLAKSEIAQGVNVQGS
                     CLAALVGVVGGGVQGTAVIMLDEGGFRATVSAMSGGMITPNLEDPVSMSVIGELANMV
                     SGRALIQAALEGTEVTPPQLISGAGIKTIPNQSPGVRSFTLPFKLSAGGTLFLVLSFS
                     V"
     gene            complement(3735..>4771)
                     /locus_tag="PK442_02715"
     CDS             complement(3735..>4771)
                     /locus_tag="PK442_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOO86592.1"
                     /translation="SLVVGAASIGDPRDLGRIGIKTIAFYLVTTAVAITIGLLLGNFL
                     QPGVGLAIGEGAAFKAPEAPSIKNVIMGLFPANPLKAAVDGNMLQIIVFALFLGVSAV
                     LAGEKGKPFINFFDSLAETMYKLTGIVMKIAPYGVFALIAVTVSKYGMSVLAPFAKVI
                     LAVYVGCALQVILVYSGLITVFVKKSPFWFLKGIREAGLTAFVTRSSSATLPITMRNT
                     QENLGVSEKVSSFVLPLGATINMDGTALYQGVCALFVAQAYGLSLGMGAQLSIILTAT
                     LASIGTAGVPGAGLIMLTLVLTAVGLPLEGAALVAGIDAVLDMARTCVNVVGDACVAA
                     VVASTEGEKL"
BASE COUNT          992 a         1275 c         1512 g          992 t
ORIGIN      
        1 cgacgaagga aacaaactgc gggagaaggg agcggaattc ggcgcgacca caggacgccc
       61 gcgccgctgc ggatggctcg acctggttgc tctgcgctat gcggtcaggg tgaacggcat
      121 ctcctcgatc gccttgacaa aacttgatgt gctcaccggt caggaacgaa tccccgtgtg
      181 cacttcatat atggtggaag gacaggatga gcctcacttt ccctgcgctc ttcaacgcct
      241 tgagaaagcc tctccgcact acgaatacct cgaaggctgg catgaggaca tcgcttcctg
      301 cacgtcgtac gaacagcttc ctccgcaggc ccggaagtac gtggagcata tcgagtccgc
      361 gaccggagtt ccggtgaatc tcatcggcgt cggccccgga agggatcaga cgattctcag
      421 gaatttctga cgaagagaag ggattccaat tttcctggct gatattcgat actgttctct
      481 caaggatgcg ccggacgtct gttttgtgga ggagcgctcg tcggttgctc cttggcctcg
      541 aactctcatc cggcgcgatt tggccggaga cacgccgatg ctgtacctcg gggcgtgggg
      601 taaggatatt ttgctgggtt tcgcggctct cgggccttgt gagaaaggga ctgagctcac
      661 gaacatcgcg gtgctccctg aatacaggag aaagggaata ggttcccagc ttgtactcgg
      721 agtatccgag gttgcgctct ccctcggata ctccagaata tacctgcatg ttcgagtttc
      781 ccacgaagaa agccgtactt tttacggggg attcgacttt tctccgctct atgtcgttcc
      841 cgggtactac ctcgacggtg aagacgcgct cttcatggaa gcgctgctgc cgctttccgt
      901 ttaagcgcgg cggaagatat gcacgctcta cagagggaac acaagcagag gacaggtcgt
      961 tcccgcgaaa agcatctccg cggtgctccc gaagacgagc tctcccagct cgcccgcaac
     1021 agggagcccg atgacaagga gcgacggcgc gagttcccgt attctcgccg gaagctcctt
     1081 cgacggatcg ccgctgacga tctccgctga ggtttcatac ccccaggagg agatttcgtc
     1141 acgaacctct tcaagagctt gccggccggc attgagaact tcgggagcgt cctccgcgga
     1201 ctggggagcg agtgcatgga gcagggtgat ctttgggttc tttcgtgcct gcgcaaggaa
     1261 ttcccgaaga ttgtccagca tcagacttgt ccgggaaggt tcaaggtcaa gagcgacggc
     1321 gagggagcca aaggtatcct cctcaggaaa aaatccctcg tgttcccgca ggagaatctg
     1381 aggcgccggc gttgcccgga cgagcggcat cccgtcctcc ccgccgggaa gagggaagat
     1441 tgcgaacgtg cacttttcgg agcggatggt atcgggaaga gactcaagca gatctcccag
     1501 aaggaccagg gtcttgtagt aggattcagg ggggagcgtt cgcgcgcaca tctcttccag
     1561 gtacccctcg gcctccctga ccagctcggg agcgtcgagc cccgcggccc ggtcgactac
     1621 atgaagaatc aggaaatccg aacgatctcc cgcgacttgc ctggccgccc atgccagggc
     1681 tctttcggaa aatgaggaga cgtctgcgaa aaagagaatt cgagtgagca tgtttcatcc
     1741 ctcccatggt attgtatgtg tgctttaagt atagcgcaaa ggggttgcag cggtgaagga
     1801 ttttactgag gtaaaatgga aggccttgtc gggtgttctt cggctcttga agagaatgcc
     1861 gttgacttgg tcggtgcgct ttggagcgtc gctcgggtgg ctgctctggg cgctcgataa
     1921 gaaaaaggtg gatgaggcgg aacggcggtg tgttcgcgcg cttcaggtgg ggccgacgat
     1981 cgccagggga atagtccgca gatcctacat gaacctgggg cgttccgttg ctgagttcgc
     2041 cgcagtgccg ggaagcggta gaagtatgcg ctcgatcgtg catgttcacg gtgaggaaca
     2101 ccttcgcgag gcattggatt ccggaaacgg cgtcattttt ttctcggggc acatcggaaa
     2161 ctgggagttc ggcgcggcgg tgctggcgga gatgggatac ccgatgaacg ccatcggcgc
     2221 ggaccagcgc gacgagagag tgaccgacct tatcatcgga cttcgcgcgg ttagcggggt
     2281 cacaaccgtg agcaaaggat tcgacctcaa ggctgccata cgatgcctca agaggggcga
     2341 agcccttgcc atcctgatcg atcaggatgt tcgtgagaga ggcgtcgtcg tcccgtttct
     2401 ggggataccc gcgagcacac cctacggtcc ggcgaagatc gccgggaaaa tcggctccgt
     2461 catcctgccg agttttatgc tgcggagggg aacctcggag gagcacgatc ttcatattct
     2521 gccctccccg tggaaaaacg ggaggccgga acgggacgaa tcgatggagg aggccatggc
     2581 ccgctgcaac gatctgatca gcgagtatat ccgccgtgct cccgaccagt ggatgtggct
     2641 ctatccccga tgggcgtcga cgggagagta gcgatgtttc tcgggatcga ccccggcagg
     2701 gacaaagtcg gatgggccct tacggatgct gagggagagc ttctcctgtc ggggattttc
     2761 cgggcggggg acgcggctct ttttttcagc gcgttcgaat ccggggcatt ggagcggctt
     2821 gcgccgttca ttcttgaaaa aacctccggt gagattgcgg attttgcgtc cgccgagttg
     2881 cttatgggag acggaactgg aaaagaatac cttctctctc ttgcgcaatc cgcctctctt
     2941 ggagtaaagt tagtgtctga aagagagaca acgctcctgg cgaggaaact ctattggaaa
     3001 ctccatcctc cgcgcggtgt gtggagactg ctttccgccg gtctccgcgt tcctccgagg
     3061 gatatagacg atcttgccgc atgggctatc gttcttcgct actgcggtgt gcagtcgaag
     3121 gagaagtaga aagggaaggg tgaaagcaaa tgggaacgga gaagctggcg acgcttgtta
     3181 atacattcgg gtcttctctg atgacggtca gcaaggagat gggcgttgag ctgactctcg
     3241 cgaagtccga gatcgcccag ggagtgaatg ttcaggggag ctgccttgcc gcgcttgttg
     3301 gcgtcgtcgg aggtggcgtc cagggaaccg ccgtgatcat gttggacgag gggggcttcc
     3361 gggcgacggt atccgcgatg tccggaggta tgattacgcc gaaccttgaa gatcctgtct
     3421 cgatgagcgt catcggcgag cttgcgaaca tggtcagcgg acgcgccctg attcaggctg
     3481 cgcttgaggg gaccgaggtg acgcctccgc agctgatttc cggcgcggga atcaagacga
     3541 ttccgaatca atctcccgga gttcgcagtt tcacgctccc cttcaagctc tcggcggggg
     3601 ggacgctttt cctggtactc tcgttcagtg tatagttcca aacgtgcggg tttttctttg
     3661 aagaaaaaag aggacgggga gataccgtcc tcttcgtgtt tgttggtgag cttcagtagt
     3721 tcgcccacag gcgattacag tttctctcct tcggtggaag ccacaaccgc ggccacgcac
     3781 gcatcgccga cgacgttgac gcaggttctc gccatgtcga gcactgcgtc gatgcctgcc
     3841 acaagcgccg cgccttcgag cggaaggccg acggcggtga ggacgagcgt gagcatgatg
     3901 agcccggccc ctggcacgcc cgccgttccg atcgaggcca gcgtcgcagt caggatgatg
     3961 ctcaactggg ctcccattcc gagggaaaga ccgtacgcct gcgcgacaaa gagcgcgcag
     4021 actccctgat agagagcggt tccgtccatg ttaatcgtcg ctccgagcgg gaggacgaag
     4081 gaagaaacct tttcggagac gccgagattc tcctgggtgt ttctcatggt gatcggaagc
     4141 gtcgcggagc tggagcgagt gacgaaggcc gtcagtccgg cttcacgaat ccccttcaag
     4201 aaccagaagg gagacttctt gacgaatacc gtgatcaggc cggagtacac aaggatgacc
     4261 tggagcgcgc acccgacgta gaccgcaaga atgaccttgg cgaacggagc gagaaccgac
     4321 atcccgtact tggaaacggt cacagcgatg agggcgaaga cgccgtaggg ggcgatcttc
     4381 atgacgatgc cggttagttt gtacattgtt tcggccagtg aatcaaagaa attgatgaac
     4441 ggtttgccct tttctccggc gaggactgcg gaaactccca ggaagagagc aaagacgatg
     4501 atctgcagca tgttgccgtc aacagcggcc ttcagcggat tcgcggggaa gagacccatg
     4561 ataacgttct tgatgctcgg agcttcgggc gccttgaatg ccgcgccttc tcctattgca
     4621 aggccgactc cgggctggag gaagttgccg agaagaagcc cgatggtgat cgcgaccgca
     4681 gtggtgacaa gatagaatgc gatggttttg atgcctattc tgccgagatc gcgaggatcg
     4741 cctatcgaag ccgccccgac gacaaggctg g
//
LOCUS       DAOSWW010000083         4767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_12083_length_4767_cov_3.501698, whole genome shotgun sequence.
ACCESSION   DAOSWW010000083 DAOSWW010000000
VERSION     DAOSWW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12083_length_4767_cov_3.501698"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PK442_02720"
     CDS             <1..818
                     /locus_tag="PK442_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86593.1"
                     /translation="LWKDLGKAMDKFTQILGFLMLALVFYVVFVTKPPVMETVHEMIR
                     PLTSLDILKEYKWLVVLTLVGGTVGGYISFSGAHRIIDAGIVGKENIGQISKGSINGI
                     AITGVMRYLLFLAFLGVVLTGTPIDMTNPVASAFQIGAGALGFRIAGVVLWAAAITSV
                     VGASYTSVSFLRTLFKVVDNYYRYWIIAFIVASTSILTTVGRPVALLIVAGSLNGLIL
                     PLSLGCVLMAAHNKNIVKDYMHPVWMTALGWVIVVFTAWMGFNSFAGIMKLFQ"
     gene            856..1626
                     /gene="pxpB"
                     /locus_tag="PK442_02725"
     CDS             856..1626
                     /gene="pxpB"
                     /locus_tag="PK442_02725"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HOO86594.1"
                     /translation="MNEKKYPRLLTAGDGCVVVEFGDVIDMEINSRVASLAAAVRKAE
                     FPGFLDTVPTYRSLAVYFNPVTTDVDALYSKLGKMASALGTGKGSESRRVIVVPSLYG
                     GEHGPDLADVAEHTGLTPEEVVKRHTANDCYCYMLGFTPGFAYLGGMDPSLETPRLKN
                     PRELIPAGSVGIAGKQTGIYSISSPGGWRLIGRTPMRMFDPDRNPAIFLEAGMWVRFR
                     AVGKAEFDEIEAATAARTYRPEILEEVSPRLVGAGEVL"
     gene            1629..2723
                     /locus_tag="PK442_02730"
     CDS             1629..2723
                     /locus_tag="PK442_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HOO86595.1"
                     /translation="MAALAFSVQMPGMLTTVQDSGRWSYQGKGMPVAGAMDMQSFALG
                     NILVGNDENAAALEVTLLGPTLSVTEGEGVAAVTGAELGFTVNGEPIANWTVVTIREG
                     DILSFDGPRSGCRAYLCVSGGIDVPMVMESRSTYTRAKVGGLEGRALKKGDAVRCGEP
                     APLWNRCEGLVCPEALRPLRDPAAPLRVIPGPQDDLFTEKGLASFYGTEYVISNSADR
                     MGYRMDGEPIEHTGAADIISDAIPLGAVQVPGHGQPIVMLADRQTTGGYTKIGVVCSV
                     DVAALSQRLPGAKVRFSKIAVKDAVGLVRAEARTRDELRKLRAAWRSSARVSSAPVPE
                     AVAAPTGGEAILRVDGKEHVISWEEIGEVE"
     gene            2723..3496
                     /locus_tag="PK442_02735"
     CDS             2723..3496
                     /locus_tag="PK442_02735"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOO86596.1"
                     /translation="MFKIDLNSDLGESFGPWRMGMDREVMEHVSSANVACGFHAGDPE
                     VMIRTVQAAKEASVAVGAHPGHPDLQGFGRRTMGCTPDEVYAFTLYQIGAIQAVCTAQ
                     GVHLEHVKAHGALYNQAAKDLAMAKAIARATRDAGGNLILLGLANSLFEQAAADEGVL
                     YAAEAFADRGYMDDGSLVPRSKPGAFIHDPDEAAMRMVRLVKEGVVRSAEGNDIKLKA
                     HSVCLHGDNPSAVELARHIRKTLQDNGVVISTIREVLEG"
     gene            3493..4290
                     /locus_tag="PK442_02740"
     CDS             3493..4290
                     /locus_tag="PK442_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HOO86597.1"
                     /translation="MKASDYANSSPREVRQLIREGKWTLPTPGMCKGHVQGNLVVLPR
                     DLAYDFLVFAQRNPKPCPILDVTEPGDPEPKIVAPGADISTDIPRYRVWKDGVCIDEP
                     TDVKKYWRDDLVGFLLGCSFSFEGALLEAGIPVRHIEQNRNVPMYVTNIQCTPAGALN
                     GPVVVSMRPIPAAMVPKAVLCTGRFPAVHGSPIHIGDPSQIGIADIDKPDLGDAVDIH
                     PGEVPVFWACGCTPQAAIMAVKPPFCITHSPGHMFVADPKDADYAVF"
     gene            complement(4386..>4767)
                     /locus_tag="PK442_02745"
     CDS             complement(4386..>4767)
                     /locus_tag="PK442_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO86598.1"
                     /translation="GLVKEYQVGWWTMLIGIQVVWFLLGFVIDAWSILMITSPIILPL
                     LPIYGFNPLWLGVLYAVNTQTGYLTPPFGTMLFMMKGIAPKGITMGDIYRSVIPFVIT
                     QLVCLALCIIFPPLITWLPSILFN"
BASE COUNT          901 a         1447 c         1510 g          909 t
ORIGIN      
        1 ttctctggaa ggacctcggc aaggcgatgg acaagtttac tcagatcctc ggctttttga
       61 tgctcgctct cgtcttctac gtcgtcttcg ttacgaagcc tcctgttatg gagacggtgc
      121 atgagatgat tcgccccttg accagcctgg atatcctgaa ggagtacaag tggctcgttg
      181 ttctcaccct cgtcggcggc acggtcggcg gctacatctc cttctccggc gcgcaccgga
      241 tcatcgacgc cggaatcgtc ggcaaggaga atatcggcca gatcagcaaa ggctccatca
      301 acggcatcgc catcaccggc gttatgcgct atctgctttt cctcgccttc ctcggcgtgg
      361 tgctcaccgg aacccccatc gacatgacca accccgtcgc ctcggccttc cagatcggcg
      421 caggcgccct gggcttccgc atcgccggag tcgtcctctg ggccgcggcc atcacttcgg
      481 tcgtcggcgc gtcctacacg tcggtctcct tcctgaggac gctcttcaag gttgtcgaca
      541 actactaccg ttactggatc atcgcgttca tcgtcgcctc cacatccatc ctgaccaccg
      601 tcggccgtcc ggtcgccctg cttatcgtcg ccggttcgct caacgggctg atccttcctc
      661 tgtcgctggg ctgcgttctc atggcggcgc acaacaagaa catcgtgaag gactacatgc
      721 accccgtatg gatgacggcg ctcggctggg tcatcgtcgt cttcaccgcc tggatgggat
      781 tcaactcctt cgccgggatc atgaagctgt tccagtagga agaagaaaga tatttatgaa
      841 ggaggcggtg tccgcgtgaa tgagaagaag tatccgagac tgctgacggc gggcgacggc
      901 tgcgtggtcg tcgaattcgg cgacgtcatc gatatggaaa taaactcgcg cgtcgcctcg
      961 ctcgccgcgg cggtacggaa ggcggaattc cccgggtttc tggataccgt cccgacgtac
     1021 cgctcgctgg cagtctattt caatccggtc acgaccgatg tcgacgctct ctactcgaaa
     1081 ctcgggaaga tggcctcggc gctgggaaca ggtaaaggaa gcgaatcgcg ccgggtcatc
     1141 gtcgtgccct ccctctacgg cggagagcat ggtcccgatc tggcggacgt cgcggagcac
     1201 accggcctga cgccggaaga ggtcgtcaag cggcacacgg cgaacgactg ctactgctat
     1261 atgctcggct ttactccggg cttcgcctac ctcggcggaa tggacccgtc gcttgaaacg
     1321 ccgcggctga agaatcctcg ggagctcatc cctgcgggtt ccgtgggcat cgcaggcaaa
     1381 cagacgggaa tctactcgat ttcaagcccg ggagggtggc ggctgatcgg ccgcacgccg
     1441 atgcggatgt tcgaccccga ccggaatccc gccatcttcc tggaggcggg gatgtgggtg
     1501 cgcttccgtg cggtcggcaa ggccgagttc gacgaaatag aagcggccac ggcggcaaga
     1561 acataccggc cggaaatact ggaagaggtc tcgccccggc tggtcggggc cggggaggtg
     1621 ttgtgattat ggcagctctt gctttttcgg tacagatgcc cgggatgctt accacggttc
     1681 aggactccgg acgatggagc taccagggga agggaatgcc ggtggcgggg gccatggata
     1741 tgcagtcgtt cgcgctcggc aacatcctcg taggcaacga cgaaaacgcc gccgctctcg
     1801 aagtcaccct gctgggaccc accctctccg taacggaggg ggaaggggtt gccgccgtca
     1861 cgggcgcgga gctcggtttc accgtcaacg gcgagcccat cgcgaactgg actgttgtga
     1921 caatacgcga gggagacatt ctctccttcg acggtccgcg ctccgggtgc agggcgtacc
     1981 tctgcgtctc cggagggatt gacgtcccaa tggtcatgga gagccgctcc acatatactc
     2041 gcgcgaaggt gggcggtctg gaagggcgcg cccttaaaaa aggcgatgcg gtgcgatgcg
     2101 gcgaaccggc gcccctctgg aatcgatgcg aagggttggt ttgtcccgag gcgctgcgtc
     2161 ctctccgcga tcccgcagcc ccgctgcgcg tcattcccgg tcctcaggat gacctgttca
     2221 ccgagaaggg gcttgcctct ttctacggca cggagtatgt catctccaac agcgccgacc
     2281 ggatggggta taggatggac ggcgaaccga tagagcatac cggggctgcc gacatcatct
     2341 ccgacgccat tcccttggga gccgtgcagg tccccggcca cggacagcct atcgtcatgc
     2401 tggccgaccg gcagacgaca ggagggtaca cgaagatagg cgtcgtgtgc tcggtcgacg
     2461 ttgccgcgct ttcgcagcgt cttcccggag cgaaggtgcg cttttcgaaa attgcagtaa
     2521 aggacgccgt cggccttgtg cgcgcagagg cgcggacgag ggacgagctg aggaaactcc
     2581 gggcggcgtg gcgctcaagc gcgcgcgtat cctctgcgcc cgtaccggaa gctgtcgcgg
     2641 cgccgaccgg cggcgaggcg attctccgcg tcgacggaaa ggaacacgtg atctcctggg
     2701 aggagatcgg ggaggtggaa tgatgttcaa aattgacctg aacagcgacc tcggcgagag
     2761 cttcgggccg tggcgcatgg gaatggatcg cgaagtgatg gagcacgtca gctccgcaaa
     2821 cgtggcgtgc ggtttccacg cgggcgatcc cgaagtaatg atccgcacgg tgcaggcggc
     2881 caaagaggcc tccgtcgccg tgggagcgca ccccggacac cccgaccttc aggggttcgg
     2941 acggcggacg atggggtgta cccccgatga agtgtacgca ttcacgcttt accagatcgg
     3001 cgcgatccag gccgtctgca ccgcgcaggg cgtgcacctc gaacatgtga aggcgcacgg
     3061 cgcgctctac aaccaggctg cgaaggacct ggcgatggcg aaggctatcg ctcgcgccac
     3121 tcgcgacgcg ggcggcaatc ttatccttct tggtttggcc aactcgctct tcgagcaggc
     3181 tgccgcagat gaaggcgtgc tctacgcagc cgaggcgttc gccgacagag ggtatatgga
     3241 cgacggcagc ctcgtgccgc gcagcaaacc gggcgctttt atccacgatc cggacgaagc
     3301 cgccatgcgg atggtccggc ttgtgaaaga gggagtcgtc cgctccgcgg agggaaacga
     3361 catcaaactg aaggcgcatt cggtctgcct gcacggggat aacccctcgg cggtggagct
     3421 cgcccgtcat atcaggaaga cgttgcagga caacggcgtc gtcatcagta ctattcggga
     3481 ggtgcttgaa ggatgaaagc gtccgattat gcgaacagct cgccccgcga ggtgcgccaa
     3541 ctgatccgtg aagggaagtg gacgcttccc acgccgggga tgtgcaaggg acacgtgcag
     3601 ggcaaccttg ttgtgctgcc tcgcgacctt gcctacgact tcctcgtctt tgcacagaga
     3661 aatccgaagc cctgccctat cctcgacgtc accgagccgg gcgatcccga gccgaagatc
     3721 gtggcgccgg gagcggacat ttcgacggac attccgcgct atcgcgtctg gaaggacggc
     3781 gtctgcatcg acgagcccac cgatgtaaag aagtactggc gcgacgatct cgtcggtttt
     3841 ctgctggggt gttcgttctc cttcgaggga gcgctgcttg aggcgggcat tcctgtgcgg
     3901 cacatcgagc agaaccggaa cgtcccgatg tatgtcacca acattcagtg cactcccgcg
     3961 ggcgcgctca acggccccgt ggtggtgagc atgcggccga ttcccgccgc gatggtcccc
     4021 aaggcggtcc tctgcaccgg ccgtttcccg gcggtgcacg gttcccccat ccatatcggc
     4081 gacccgtcgc agatcggcat cgccgacatc gacaagcccg accttggcga cgctgtcgac
     4141 atccatccgg gagaggtccc cgtcttctgg gcgtgcggct gcacgccgca ggcggccatc
     4201 atggcggtga agcccccgtt ctgcatcacg cactctccgg ggcacatgtt cgtcgccgac
     4261 ccgaaggatg ccgactacgc ggttttttag aaatcggaga agcagtttcc agtacaaaag
     4321 aagaggggac cggaaggtct cctcttcttt tatttgttct ttttcccctt ttgttccccg
     4381 cgctcctaat tgaagagtat gctgggaagc caggtgataa gcggcggaaa gatgatgcac
     4441 aacgccagac acaccaactg cgtgatcacg aaggggatga cggagcggta gatgtctccc
     4501 atcgtgattc ccttgggtgc gatgcccttc atcatgaaga gcatggtgcc gaagggcggc
     4561 gtcaggtatc ccgtctgcgt gttcaccgcg tagaggacgc ccagccacag agggttgaat
     4621 ccatagatag gcagcagggg gagaatgatg ggcgaggtga tcatgaggat gctccacgcg
     4681 tcgatcacga aaccgagcag gaaccagacg acctggatgc ctatcagcat cgtccaccag
     4741 ccgacctgat actccttgac gagtccg
//
LOCUS       DAOSWW010000084         4605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_12698_length_4605_cov_3.560440, whole genome shotgun sequence.
ACCESSION   DAOSWW010000084 DAOSWW010000000
VERSION     DAOSWW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12698_length_4605_cov_3.560440"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PK442_02750"
     CDS             <1..1173
                     /locus_tag="PK442_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO86599.1"
                     /translation="GKRFEEVRAESLGGFLPRLSDDGIDLLFWSSRADGTVAGFLVDV
                     DVLKDRIAGVLPEILTDTRILTVLDERGEPLLAPETKELPDWRRPFVAREISPLLPRW
                     EAGAWLADPTDAASRARFAARAVWLLVGALFFVILCGGALILWMLSSEMRRARRKTTF
                     VANVSHELKTPLTSIRLFAEMLLSGRQKDEERRKEYLRTMVSEAERLSRLVENVLAFS
                     RGGGGGESATEPLDLSELTRETLSQLEPNLLKNGFACSFSTGGPAPVRGSPERLRQVL
                     MNLLSNAEKYSGDVREIGIKCGNESGYAFVEVADRGPGVPSSQKEKIFQEFFRGDNSL
                     TASRSGAGLGLSIARSIARRHGGDVSYMPRSGGGSVFSLRIPLEEAAAGSGQGEEK"
     gene            1170..1877
                     /locus_tag="PK442_02755"
     CDS             1170..1877
                     /locus_tag="PK442_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO86600.1"
                     /translation="MNERILIAEDDFAILTGVADLLESEGYEVETACDGLTALERYRA
                     VRPDLVLLDVMMPGMNRYDVCRAIRTEDRFIPLLMLTAKSSEIDKVIGLELGADDYIV
                     KPFGMAELLARVRSALRRGALSSTARTEGAASSTLSFGDVTVNFESMTGQKNGVEFAL
                     TPKEAALLRFLTEREGKVVSRNTLLDDVWGIDCEVTTRTVDQHVVRLRQKIEDDPSRP
                     VFLCTVHGAGYRFVLEQ"
     gene            2040..4223
                     /locus_tag="PK442_02760"
     CDS             2040..4223
                     /locus_tag="PK442_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86601.1"
                     /translation="MARVHGMHADENSPFSSGHLKRTTPRPIAISWHNLSTQEYYPFF
                     KTDASFRRLLECNPLFARLFGVEGSEAFGSMPAAEVLFPSAALRETILERFEKGFLDR
                     FEAPFRRKDSSSFWGELSAIVSPDGSCICGILRDVTERKTNELWLKEQNSSLSMLAEI
                     SADLMAELDTDSLLQKVLRYATSAMNTEHGSISFVDFTTKERYVRWSQGPILSSFQGI
                     RIPLGMGLGGEVIAAGKRKIVKDYRNFSNRLPDHDFDCIGTTVGVPLRWKDEVFGYLS
                     IYYEGEPREIEDARLEHLDQFAFLASLALHNARLYEDARLEIAERRRLEEELRKATLE
                     AQRANEAKSDFLARMSHEIRTPLNILTGVSDLLLEREVDSELRENLSMISQAGKFLTE
                     MVNDILDFSKIEAGMIEVERMPFSLSALLGDIAPMFVFHAERSGIAFRVSADPTLPDV
                     FTGDSLRLKQILFNLLGNAMKFTKRGFVRLAVTGVPLGEFSYEIRFTVSDTGVGMMPD
                     VLDSIFEPYHQGELSVARRFGGSGLGLPISRKLAELMGGVLSVESALGRGSDFTLSLP
                     LEVARTPSDSKGTMEEEGTALPEKLSILVADDNTVNRTLLSKLLSLPGWTVLMAADGQ
                     EALEIVRKDAPDVIILDLEMPRMDGCAAAKAIRKHEVESGKRSFILGLTGYTRSESAP
                     LCADAGMDDCLTKPARKFTLLAGIANYFSSGGVWNTLEDDEENEE"
     gene            4213..>4605
                     /locus_tag="PK442_02765"
     CDS             4213..>4605
                     /locus_tag="PK442_02765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO86602.1"
                     /translation="MKNKNVLYEQLRLVENNPLNTEALARMIADPEDLHLVWPVAEYP
                     FNHDQWRAELDPANGTRSFLVYEGERMVGHGALAPCRGDEDSVMVRFLYIAPDMRNRG
                     AGRRLLSLLEARAKDAMGARRLILKVRTY"
BASE COUNT          966 a         1201 c         1427 g         1011 t
ORIGIN      
        1 gggaaacgct tcgaagaggt gcgggccgaa tctttggggg gctttcttcc aaggctttcg
       61 gacgacggca ttgatctgct cttctggagt tcgcgtgccg acggaacggt cgccggattc
      121 cttgtggatg ttgatgtgtt gaaagacaga atcgccgggg tgctccccga gatactgacc
      181 gacacccgta tactcaccgt tctggacgaa cggggcgaac ctcttctcgc cccggaaacc
      241 aaagagcttc cggactggcg gcgcccgttc gtcgccaggg aaatttcgcc ccttctgcct
      301 cgctgggagg ccggagcgtg gctcgccgac cctacggacg ccgcttcccg cgcccggttc
      361 gccgctcggg ccgtctggtt gcttgtggga gcgctctttt ttgtgatact ctgcggcggg
      421 gcgcttattc tctggatgct ctcatcggag atgcgtcgtg cacgccgaaa gacaaccttt
      481 gtcgccaacg tctcgcatga gctgaaaacg cctctcactt ccatccgcct cttcgccgag
      541 atgctgctct cggggcggca gaaggacgaa gaacgtcgaa aagagtatct tcggactatg
      601 gtctccgagg cggagcgtct ttcccgtctg gtagagaacg tgctcgcgtt ttcccgcgga
      661 ggtggcggcg gagagtccgc gactgagccc cttgatcttt ccgaattgac gcgcgagacc
      721 ctttcgcagc tggagccgaa ccttttgaaa aacggttttg cctgttcttt ttcaaccggc
      781 ggaccggcgc ctgtgcgcgg gagtccggag cgtctccggc aggtgctgat gaatctgctg
      841 tcgaatgcgg agaagtactc cggcgatgtc cgcgagatcg gcataaagtg cggaaacgag
      901 tccgggtatg ccttcgtgga agttgcggac agggggccgg gggttccctc ctcgcagaag
      961 gaaaagatct tccaggagtt ctttcgggga gacaattcgc tgacggcgtc gagaagcggg
     1021 gccggactgg gactttcgat agcgcggtcg atagcgcggc gccacggagg cgacgtatca
     1081 tatatgccaa gaagcggcgg gggcagcgtc ttttcgctga ggataccgct tgaggaagcg
     1141 gcggcaggct cgggacaagg ggaggagaaa tgaacgaacg cattctgata gcggaggacg
     1201 acttcgccat cctgaccggg gtggcggatc ttctggagag cgaagggtac gaagtggaga
     1261 ccgcctgcga cgggcttacg gcgctcgaaa gataccgggc cgtgcggccc gaccttgtgc
     1321 tcctggatgt gatgatgccc ggaatgaacc gatacgacgt ctgccgcgcc atccgtacgg
     1381 aagaccgctt cattccgctg ctgatgctta ctgccaagag ctcggagatc gacaaggtga
     1441 tcgggctcga actcggggcc gacgactata tcgtcaaacc gttcggaatg gccgagcttt
     1501 tggcccgcgt ccggtcggcg ctgagaagag gcgctctgtc ctcaacggcg cggacagagg
     1561 gagcagcctc ttcaacactc tcgttcgggg atgttacggt gaatttcgag agcatgaccg
     1621 ggcaaaagaa cggcgttgag tttgcgctga ctccgaaaga ggcggcgttg cttcgttttc
     1681 tcacggagcg agagggaaag gtcgtctcca gaaacactct tctggacgat gtgtggggga
     1741 tcgactgcga ggtgacgacg cggactgtcg accagcacgt ggtccggtta cggcaaaaga
     1801 ttgaggacga cccctcgagg ccggtctttc tttgcactgt gcacggggcg gggtatcgat
     1861 tcgttctgga acaatgagaa gaacgttttt acatgcaaag atgttcaaag atgattcctg
     1921 cgaatcatcg aagaggcatt cctgaatcat ttccaatgtg gtattttaat aagcatagtg
     1981 gagagtatgt aaagaaggga attgtttcaa agaatgaaac attacctggg ggtggatgtg
     2041 tggcacgtgt tcatggaatg cacgctgacg aaaactctcc gttctcttcg gggcatttga
     2101 agagaacgac tccccgccct atcgccatat cctggcacaa tctgagtacg caggaatatt
     2161 accctttctt caagaccgac gcatctttca ggcgtcttct ggaatgcaat ccgctctttg
     2221 cccgcctctt cggcgtcgag ggcagcgagg cttttggaag catgcccgca gccgaggtcc
     2281 ttttcccttc cgccgcttta cgggagacga ttctcgaacg tttcgagaag gggtttttgg
     2341 acaggttcga ggcgcccttc agacgaaaag attcttcgtc cttctggggc gagctttcgg
     2401 cgatcgtttc acccgacggc agctgtatct gcgggattct tcgcgatgtg acggagcgca
     2461 aaacaaacga actctggctg aaggagcaga acagttcgct ctcgatgctt gccgagatca
     2521 gcgcggatct tatggccgag ctggatacgg attcccttct ccagaaagtg ttgcgatacg
     2581 ctacatcggc gatgaacacc gagcacggct ccatatcgtt cgttgatttc accacaaaag
     2641 agcgctatgt ccgttggtcg caggggccga tcctctcctc gttccaagga atccggatac
     2701 ctttgggcat ggggctcggc ggcgaggtga tcgcggccgg caaacgcaaa atagtcaaag
     2761 attaccggaa ctttagcaac aggctgcctg atcatgactt cgactgtatc ggaacgacag
     2821 tcggggtacc gctgcgctgg aaggacgagg ttttcggcta cctttccatt tactacgaag
     2881 gagagccgcg ggaaatcgag gatgcgcggc tggagcatct cgatcagttc gctttcctgg
     2941 cttcgttggc gttgcataat gcaagactct acgaagatgc gaggttggag attgcggaac
     3001 gccgccgcct ggaggaggaa cttcggaagg cgacgcttga ggcgcagcgc gcaaacgagg
     3061 cgaagagcga ttttctggcc cggatgagcc atgaaatacg aacgccgctg aacatcctca
     3121 ccggagtgag cgatctgctg cttgaaagag aggtcgattc cgaactgcgc gaaaatctct
     3181 cgatgatctc ccaggccggc aagtttctca cggagatggt gaacgacatt ctggacttct
     3241 caaaaataga agcgggcatg atcgaggttg agcggatgcc tttctccctg agcgcgcttc
     3301 tcggggatat tgcgccgatg ttcgtctttc atgcggagcg tagcggtatc gcctttcgcg
     3361 tgtccgccga tccgacgttg ccggacgtct tcacagggga ttcgctgcgc ctgaagcaga
     3421 ttctctttaa tctcttgggg aatgcgatga aatttacaaa aagaggtttc gtacgtcttg
     3481 ccgtgaccgg agttcctctc ggcgaattca gttacgagat ccggttcacc gtctcggaca
     3541 caggcgtggg tatgatgccg gatgttcttg attcgatctt cgagccgtat caccaaggcg
     3601 agctctccgt tgcccgtcgg ttcggcggtt ctggcttagg gctgccgatc agcagaaagc
     3661 tggcagaact tatgggagga gtcctttcgg tggagagcgc tctcgggaga ggttccgact
     3721 ttacgctttc gcttcccctg gaggttgccc gaacgccatc tgattccaag ggaacaatgg
     3781 aagaggaagg aaccgcgctt ccggagaagt tatcgattct tgtggccgac gataacacgg
     3841 taaacaggac gcttctctca aagctgcttt cgcttccggg gtggaccgtc ttaatggcgg
     3901 cggacggaca ggaagccctg gagattgtac gaaaagacgc ccccgacgtc attatcctgg
     3961 accttgagat gccacgaatg gacggctgcg ctgcggcgaa agccatacgg aagcacgaag
     4021 tcgagtcggg aaagcgttcg tttatcctgg gattgacggg gtatacccgt tccgaaagcg
     4081 cccctctctg cgcggatgcg ggtatggacg actgccttac aaaacctgcg aggaaattta
     4141 cactgctggc gggcatagcg aactactttt cttccggcgg ggtgtggaat acgcttgagg
     4201 atgatgaaga gaatgaagaa taagaacgtc ttgtacgaac agctgcgact tgtagaaaac
     4261 aacccgctga atacggaagc tcttgcccgg atgattgcgg atccggaaga tcttcatctt
     4321 gtttggccgg tagccgaata tcctttcaac cacgaccaat ggcgggccga gttggaccct
     4381 gcaaacggga cgcgctcatt cctcgtgtac gagggggaac gaatggtcgg acacggcgcg
     4441 ctcgcccctt gccggggcga tgaagattcc gtgatggtgc gttttctgta tatcgccccc
     4501 gatatgcgga acagaggggc gggaagacgc ttgctctctc ttctggaagc gcgcgcgaag
     4561 gacgctatgg gagcgcggcg ccttatccta aaagtcagaa catac
//
LOCUS       DAOSWW010000085         4589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_12761_length_4589_cov_2.986326, whole genome shotgun sequence.
ACCESSION   DAOSWW010000085 DAOSWW010000000
VERSION     DAOSWW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12761_length_4589_cov_2.986326"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..216
                     /locus_tag="PK442_02770"
     CDS             46..216
                     /locus_tag="PK442_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86603.1"
                     /translation="MDMLTINSVVMILYYFITTFFLLAMVRNFIVTRDVQEALLYAVV
                     MIPFVLRLLRLK"
     gene            233..1396
                     /locus_tag="PK442_02775"
     CDS             233..1396
                     /locus_tag="PK442_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HOO86604.1"
                     /translation="MNIRTAKLIILLFAGSCAAIAGSLFKEHMEYKEAVVASPFLSEV
                     RKLSDYSDAVKGSVNDANVYIFNGPTPGGTVVLLGGTHPEEPVANLSAQIFTENVHPV
                     QGRVIVINRINTSASMATRMGEAYPRFYHISTPWGVKKWRYGDRLATPLHSWPDPEVY
                     VHYPSGQNLAYMDVRNANRTWPGRPDGLLCERTNYALMELIRKEKADIVMDFHEAELE
                     YAVENTIVVHEKGQSVAAMASMMLASQTFDVSIGMEFSPKALHGLSHREVGDHSDAVS
                     YLVEVAEPMLDRIRGITDEELLMSGKDRFVMKAGEYKLLYSPIDESGWPVEKRTARHV
                     ATLMTILQVNNMLAPEKTIILEGIPDYREIMGNGLGAFFQNPDEAPQERVFYD"
     gene            complement(1494..2738)
                     /locus_tag="PK442_02780"
     CDS             complement(1494..2738)
                     /locus_tag="PK442_02780"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOO86605.1"
                     /translation="MNPQAMFLQQLEQAVPYVTVEPEYLEILRHPKEVLELSLPVRLD
                     GGGVKVFKAWRSHHNNALGPYKGGVRYHAEVSRDEVVALSSWMTIKCAIAGLPYGGGK
                     GGISVDIRELSRNELETLSRAYAFGLARFTGTDYDIPAPDVYTNPQIMSWFLDTYEKV
                     KGWSESSAYTGKPPVLGGSLGRSDATGKGGVFILREGLAKLGLLGKKDLKVGIQGYGN
                     AGTRAHRFVEDDLGLTVVAVSDSGAALYNPKGLPYREVSKHKNNTRSLKGFQGAEELF
                     PDLLFGLDMDILILAALGGAVDAQNAPDIKAHVLLEIANGPVTPDAEALLTDKGVTII
                     PDVLANAGGVIVSCFEWIQGRSGDVWTEEYVFQKLDERLTLSFKDVMKIAEDRKISAR
                     KAAYVKAVGRIVDAMRFRGIWP"
     gene            complement(2921..4198)
                     /locus_tag="PK442_02785"
     CDS             complement(2921..4198)
                     /locus_tag="PK442_02785"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOO86606.1"
                     /translation="MSVVKRTSSNVLLDTALKNFYAAAEEMGLEEGLIDILSHSERKT
                     SVSIPVEMDDGSVKVFDGYRVLHSSAIGPGKGGVRFHPEVNVEECEALAMMMTWKCSL
                     AGIPYGGGKGGVSCEPLELSKKEKERISRTWAARMAPVIGAWTDVPAPDVNTGGPEMV
                     WFMDTISKMKNQLEPAIFTGKPIPLWGSKGRTAATGYGVATCALELLKNLGKDPKAIS
                     VSIQGFGNVGTYAALTMVDAGSKVVAISDITGTYFAEKGLDIKKAIEHVNNHPKHLLE
                     GFTCDGCQKLDLPEALFVNCDILIPAALEGVINGKNADKVKAKYIVEAANGPVTPEGD
                     AILDAKGVLVVPDFLANSGGVIGSYFEWAQDLGGFFWSEEEYNERLIRIMKDNFQRVW
                     GYAKDKNVKMRRAAFMAAIQRVADAVRMRGVFL"
     gene            complement(4392..>4589)
                     /locus_tag="PK442_02790"
     CDS             complement(4392..>4589)
                     /locus_tag="PK442_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86607.1"
                     /translation="SIEDGKPPVEAEFQKIEQDGVVLWVPSFMEFKNNEILIVQRGFL
                     WSAYLMLVGVLSESEESCCNS"
BASE COUNT         1094 a         1280 c         1114 g         1101 t
ORIGIN      
        1 ggtgtacagc aaggagattt cccaagtgat cggaggttga cgtctgtgga tatgcttacg
       61 ataaattcag tggtgatgat cctgtattac ttcatcacaa ctttttttct tcttgcgatg
      121 gttcgaaact tcatcgtgac acgggatgtt caagaggctc tcctgtacgc cgtcgttatg
      181 attccctttg ttttgcgcct tctgcggctc aagtagaaag gggccttaga gaatgaatat
      241 tcgcactgca aaactcatca ttcttctttt cgcaggctct tgcgctgcga tcgcaggatc
      301 tctttttaaa gagcatatgg agtataaaga agctgtcgtc gcgagcccct ttctctcgga
      361 agtcagaaaa ctcagcgatt acagcgatgc agtcaaaggc tcggttaacg atgcgaatgt
      421 atatattttc aacggtccga cccccggcgg gaccgttgtc ttgctcggag gaactcaccc
      481 ggaagagccg gttgcgaacc tttcagctca gatatttacc gagaacgttc atcccgttca
      541 aggacgggtc atcgtcatca accgcatcaa cacaagcgcc tccatggcca cacgaatggg
      601 agaggcgtac cccaggtttt atcatatttc caccccctgg ggagtaaaga aatggagata
      661 tggcgaccgg ctcgcaaccc cgttgcactc ctggccggac cccgaggtgt acgttcacta
      721 cccgagcgga cagaatctcg cctacatgga tgtacggaac gccaaccgga catggccggg
      781 acgccccgac ggcctccttt gcgaacgtac gaattacgcg ctcatggaac tgatccgtaa
      841 agaaaaagcg gatatcgtca tggacttcca cgaagccgag cttgaatacg ccgtggagaa
      901 caccatcgtc gttcacgaaa agggacaatc ggtagcagcc atggcatcta tgatgctcgc
      961 ctcccagact tttgacgtat ctattggtat ggagttctcc ccaaaagcgc ttcacggtct
     1021 ttctcaccgc gaggtcggag atcattccga cgctgtttct taccttgttg aagtggcgga
     1081 acctatgctt gacagaattc gcgggatcac cgacgaagag ctcttgatgt caggaaagga
     1141 ccggtttgtc atgaaggcgg gagaatacaa gctcctctac tcccccatcg atgagagcgg
     1201 atggcctgtg gaaaaaagga cggctcgtca tgtcgctacg ctgatgacca ttctccaggt
     1261 gaacaacatg ctcgccccgg agaaaacgat catccttgaa ggaatcccgg attaccggga
     1321 aataatggga aacggcctgg gggccttctt tcaaaatcca gatgaggcgc ctcaagagag
     1381 ggttttttac gattgatcgt atagagagga acatctatct tcataaaccc ctctctacag
     1441 aagagcggag accgactcaa agaaaagaat ccggcctccg ctcttcttca tttttagggc
     1501 catatccccc tgaatcgcat cgcatcgacg attcttccaa ccgccttgac atatgccgcc
     1561 ttcctggcgc ttatctttct gtcctcagcg attttcatga cgtccttaaa tgaaagagta
     1621 aggcgttcgt caagcttctg aaagacgtac tcctccgtcc atacgtctcc tgatctcccc
     1681 tgtatccatt cgaagcaact gacgataacc cctcccgcat ttgcaagaac atccggaata
     1741 atcgtaacgc ctttatcggt aagcagggcc tcagcatcgg gcgtaaccgg accgtttgca
     1801 atttcgagca gtacatgcgc tttaatgtcc ggcgcattct gagcatccac agcgcctcca
     1861 agtgccgcga ggatcaaaat atccatgtca agtccgaaga gaagatcggg gaagagctcc
     1921 tcagctccct gaaatccttt aagggaacgg gtattgttct tatgtttaga gacttctcta
     1981 tacgggagcc ctttcgggtt atagagtgcc gccccgctgt ctgatactgc aacaaccgtt
     2041 agtccaagat catcttccac aaagcggtga gcacgcgtcc ccgcgttccc gtagccctgt
     2101 attccaacct tcaaatcttt cttccccaga aggccgagct ttgcaagccc ctcacggaga
     2161 ataaagacgc ctcccttccc cgtagcatcc gatcttccca gagaaccccc cagaacagga
     2221 ggtttcccgg tatatgccga cgattcgctc catcctttga ctttttcata cgtatcaagg
     2281 aaccacgaca tgatctgagg gtttgtgtac acgtccggcg ccgggatgtc gtaatccgtc
     2341 cccgtaaatc gggcaagtcc gaaagcatac gctctgctca gagtttcaag ttcgttacgg
     2401 gaaagctccc ttatatcgac agagattcct ccctttcctc ctccgtaagg gagccctgca
     2461 attgcacatt tgatcgtcat ccacgatgaa agggcgacca cttcatccct gctgacctcc
     2521 gcatgatagc gcacgccgcc cttgtaaggg ccaagagcgt tgttgtggtg gctcctccac
     2581 gccttgaaca ccttcacgcc tcctccgtcg aggcgcacag gcaaagagag ttcaagcacc
     2641 tccttcggat gcctgagaat ttccaggtac tccggctcca cagtcacata cggaaccgcc
     2701 tgttcaagct gctgtaaaaa catagcctgc ggattcacct ttcatccctc caatcttctg
     2761 gaaacgaact gctactggac gctcacaact taattgaaca gttggaattc gtgtattatt
     2821 ttatcacaca actcttttcc tgctccattt ttcaatacaa aaaaacgacc ggttgaccgg
     2881 tcgttttcag atgtatcaga aaagcggttt tggctcgaat ttagaggaag acgcctctca
     2941 ttcgcactgc atcggcgact ctctgaatcg ccgccatgaa cgctgcgcgg cgcatcttca
     3001 cgttcttgtc ctttgcgtag ccccacacgc gctggaagtt gtccttcatg atgcggatga
     3061 ggcgctcgtt gtactcctcc tccgaccaga agaaaccgcc gagatcctgt gcccactcga
     3121 agtaagagcc gataacgccg ccggagttgg cgaggaagtc cgggacaacg agaacgccct
     3181 ttgcgtcaag aatagcgtcg ccttccggag tcacggggcc gtttgcagcc tcaacgatgt
     3241 actttgcttt gactttgtca gcattctttc cgttgatgac gccttcaaga gctgcgggaa
     3301 tgagaatatc gcagttgaca aagagagctt cgggaaggtc gagcttctgg cagccgtcgc
     3361 aggtgaagcc ttcaagcagg tgcttgggat ggttgttcac atgctcgatg gcttttttaa
     3421 tgtcaagccc cttctctgcg aagtaagttc cggtgatgtc gctgatggcg acgaccttgg
     3481 atccggcatc gaccatagta agggccgcgt aggttcctac gttgccgaag ccctggatcg
     3541 agacagagat tgctttcggg tctttgccga ggttcttcag cagttcaaga gcgcaggttg
     3601 caacgccgta accggtcgcc gcggtgcgtc ctttggaacc ccacagcgga atgggtttgc
     3661 ctgtgaagat ggcgggttca agctggttct tcatcttgct gatggtatcc ataaaccaga
     3721 ccatttccgg gccgccggtg ttcacgtcag gagcgggaac gtcggtccat gcgccgatca
     3781 cgggagccat gcgggcagcc catgtgcggg aaatacgttc tttttccttc ttcgagagtt
     3841 ccagaggctc gcaggaaacg ccgcccttac cgccgccgta agggataccg gccagcgaac
     3901 acttccatgt catcatcatg gcaagcgctt cgcactcttc aacgttcact tccgggtgga
     3961 aacggacgcc gcccttgccg gggcctatcg cggaagaatg gagcacccgg tagccgtcga
     4021 agaccttcac agatccgtcg tccatctcga cagggatgga cacgctggtc ttgcgttcgg
     4081 aatggctgag aatatcgata aggccctctt caagccccat ctcctccgct gcggcataga
     4141 aatttttgag tgcggtgtct agaagcacgt tcgaggaagt acgctttaca acagacatga
     4201 aacacacctc ctgtatattt acggcctgag ccgtttgaga ccttaataac tatcatagca
     4261 ctctttttcc aaagaacaag cgagaaattg aaatacatca aaataatcct aaatacccgc
     4321 cacaaaacac aaaatctctt taacggagaa acgaagtacc ataggcttgc agcgtttggg
     4381 ttctcagaat ctcacgaatt acagcacgat tcttccgatt cactgagcac cccgacaagc
     4441 atcagatatg cagaccagag aaatccgcgc tgtacaatga gaatttcgtt gttcttgaac
     4501 tccatgaatg acggaaccca gaggacaacg ccatcctgct caattttttg aaactccgcc
     4561 tccaccggag gcttcccgtc ctctatact
//
LOCUS       DAOSWW010000086         4576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_12811_length_4576_cov_3.917496, whole genome shotgun sequence.
ACCESSION   DAOSWW010000086 DAOSWW010000000
VERSION     DAOSWW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12811_length_4576_cov_3.917496"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..105
                     /locus_tag="PK442_02795"
     tRNA            16..105
                     /locus_tag="PK442_02795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Ser,seq:cga)
     gene            168..255
                     /locus_tag="PK442_02800"
     tRNA            168..255
                     /locus_tag="PK442_02800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Ser,seq:gga)
     gene            850..1914
                     /locus_tag="PK442_02805"
     CDS             850..1914
                     /locus_tag="PK442_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86608.1"
                     /translation="MLPLLFTTLGSVTSDGGLFIMPKNRADMLAGYFLESLDETARAT
                     MRFLEQEPPVEEPSEDEENRKKKKERKLESFPKLMEGIRRLKTIAKDEDGSDLVVDRL
                     LSSIDMLFLESLTAYAYKKSALEVVRRKKKLRQEVNDTPFSPEQALLEFMKQHLERIY
                     AVQERACKAFSEENAPEKSGYTPFLLPDSVEYLDILDEEEIQREKKKKAGDLSFERAF
                     LEILSADRGLFTLPRIRSRGLPGILFLPGNAVGAYDKKADFFLLPFSALRERAFEHLL
                     NAFASFRWAWDDSGKVLNLCADLVGSRGAKPQLELEAAFRRAYSSWLQSEIRGRAVDE
                     HGESIKKALRPEGDFARFGE"
     gene            complement(1966..2964)
                     /locus_tag="PK442_02810"
     CDS             complement(1966..2964)
                     /locus_tag="PK442_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO86609.1"
                     /translation="MREILFVSNTGNTTVVIFFVLFTVILVMSGIKIVPQASRVIVER
                     LGKFHKILEPGINFIFPVLDRRKATSWIVGGEIRNTTLIDIREQVFDFPKQNVISHDN
                     VVMEINALLYFQINDPFKAVYEIANLPLALEKLTQTSLRSVMGEMDLDEIFSKRAEIN
                     ERLRVILDEASDAWGVKVTRVEIQDVNPPASVQAAMQRQMEAERSRRAMVTEAEGKKT
                     AQILEAEGHKEAQINRAEGEKQAVILDAEGRARARIRMGEAEAEAVVKVASAVKIAGG
                     DPTQYLIALKYMDTLSSVASGNENKVVYLPYEATAILGSLGGMKELFSKDKTQTNA"
     gene            complement(3041..3517)
                     /locus_tag="PK442_02815"
     CDS             complement(3041..3517)
                     /locus_tag="PK442_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOO86610.1"
                     /translation="MFELVTNWQLWVILGFALLIVEMFTPGFVLACFAIACIPPAIIT
                     YFEDFDLRAQLAVFGVSTLFIFFFMRPAVLRFLSNRKDARTSNAEALIGKKGIVIKTI
                     SPDAAQGGYVKVDGKTWWAFGPEGIEIGEGAGVVVVKVRGASLDVQFEENESSGEE"
     gene            complement(3575..4177)
                     /locus_tag="PK442_02820"
     CDS             complement(3575..4177)
                     /locus_tag="PK442_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOO86611.1"
                     /translation="MQWTAFFTYILITCNTPGPNVVLAMNTARIHGLRKTLPLIAGMM
                     SGLLLLMTFCAIFNLLLARLLPTLLPVLRTGGTVYILWLAWKIAFPKRNSLENAPAED
                     APDFRHGFVLQFLNPKVILLGLTTLSTFILPWTTSKTWLFGCGVFLTLACSNGVFLWS
                     LLGAAQKRLMTEQGRFWDYLMGGLLAWCAFSVSGLKELFF"
     gene            4325..>4576
                     /locus_tag="PK442_02825"
     CDS             4325..>4576
                     /locus_tag="PK442_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO86612.1"
                     /translation="MMKHHQDICFLLSQESERTDTANHNQGANQMTFYDRFSVAGKVA
                     VVTGAARGLGKGYALGLASAGATVLCGDVNENDAKLTADE"
BASE COUNT         1132 a         1163 c         1158 g         1123 t
ORIGIN      
        1 ttattcgggt cgtgcggaga ggtggccgag tggtcgaagg cgattgactc gaaatcaatt
       61 gggcggcttg ccgtcccgtg ggttcaaatc ccaccttctc cgccaataaa aaagtttttc
      121 cggtctggca aacttggaaa actgtgatat aatacctgcc gttatgcgga gaagtggccg
      181 agtggtcgaa ggcgggcgct tggagagcgt ctgaaccgtg aggttccgtg ggttcaaatc
      241 cctccttctc cgccattttt ttcaatatct tcccccctaa taaatctttc tcattcactt
      301 aaaaaacgaa tgtcttgtct gttcgcctgc tctgatgtat cctcttacca gagacgtacc
      361 ttagagatcg gagatgtttc agatgcagac aaaaacaact ctttcctccg ggagcttttt
      421 tatttcgcga actagggccg atatatgctc ggcatgattg gttcctccga atcgctacct
      481 gaagagcagc gtactacatt gactgccctt gcccatttcc acaaaacaag ggttctttcc
      541 ggcttgccct ctcctcaacg tttagcgtct ctccacagat ttctcgtacg ccgttttatc
      601 gatgatacta cgttctcatt gactcaggaa cagtatgagc ttgtcgattc aggagcgtat
      661 agctctgctt cgtctctctc ttcggtgcaa aggagcgacg tgtgtgcggc gctcactctg
      721 actgaatgga ttgaagacac gcgagctgcc tttgaagaag gaaaagaaga ttatgccgtt
      781 tcacacgtaa cgaaagcgat cgcttcatcc gaggcacgtt tgaacgcgct cggggggaga
      841 attcgcacaa tgctgccgct ccttttcaca acacttggga gcgttacttc cgacgggggc
      901 ctttttatca tgccgaagaa ccgtgccgat atgctcgccg ggtatttttt agaatctctt
      961 gacgaaacag cccgggcgac aatgcgtttt ctggagcagg agccgcctgt ggaggagcct
     1021 tcagaggatg aagaaaacag gaaaaagaag aaagagcgga agctggaaag ctttccgaag
     1081 ttgatggagg gcattcgacg gctgaagact attgccaaag atgaggacgg cagcgatttg
     1141 gtggtagaca gattgctttc gagcatcgat atgttattcc ttgaatcact tactgcgtat
     1201 gcctacaaaa aaagcgcttt ggaagttgtg cggcgaaaaa aaaagttacg gcaagaagtg
     1261 aacgataccc ccttctcacc tgaacaggcg cttcttgaat tcatgaagca acatctggag
     1321 agaatctatg ctgttcaaga gagagcgtgc aaagcgttct cagaggagaa cgcaccggaa
     1381 aaaagcggct ataccccgtt tctcctccct gattccgtcg agtatctcga tatactggat
     1441 gaagaagaaa ttcaacgtga aaaaaagaaa aaagcaggag atctgagctt tgaaagagca
     1501 ttcctcgaga ttctttctgc tgacaggggg ctttttactc tcccgcgaat tcgttcccgg
     1561 ggtcttcccg gcattctctt tcttcccgga aacgctgtcg gtgcatacga taagaaagca
     1621 gatttcttcc tccttccttt ttccgcgctc agagaacggg cattcgagca ccttttgaat
     1681 gcgtttgcct ctttccgctg ggcttgggat gacagcggaa aagtcttgaa cttgtgcgct
     1741 gatcttgtgg gcagcagggg agcgaagccg cagctggaac tcgaagctgc gtttcgcaga
     1801 gcctattcgt cgtggcttca gtctgaaatt cgtgggagag cagtcgacga acacggtgaa
     1861 tcgataaaaa aagcgctcag gccggaaggc gacttcgcgc gtttcggaga gtaatttctt
     1921 cagacagaag agagagggtg aataccccct cccttctgta ctgttttaag cgttcgtttg
     1981 tgttttgtcc ttagaaaaaa gttctttcat gccgccgagc gagccgagga tcgctgtagc
     2041 ttcataaggc aggtagacaa ccttgttctc atttccggat gccactgagg aaagggtgtc
     2101 catatattta agagcgatga ggtactgcgt gggatcgcct cccgcaattt tcacggccga
     2161 ggctactttc accacagcct cagcctcggc ttctcccatg cgaattctgg cccgggcgcg
     2221 tccttccgca tcaaggatca ccgcctgttt ttctccctca gcacgattga tttgcgcctc
     2281 tttatgccct tccgcctcaa gtatctgcgc ggtttttttc ccttccgcct cggtgaccat
     2341 ggcccgcctg gagcgttccg cctccatctg gcgctgcatt gcggcctgaa ccgaggcagg
     2401 cggattcacg tcctgaatct ccactctcgt cacctttacc ccccaagcat cgctggcttc
     2461 gtcgaggata acacggagac gttcgtttat ttccgcgcgt ttgctgaata tctcgtcaag
     2521 gtccatctct cccatcacgc tcctgagaga tgtctgcgtc agtttttcca gagccagcgg
     2581 caggttggca atttcgtaca ccgctttgaa cggatcgttg atttggaaat agaggagcgc
     2641 gttgatctcc atcacgacat tgtcatgcga gataacgttt tgctttggga agtcgaaaac
     2701 ctgctccctg atatcaatga gcgttgtatt ccgtatctcc cctccgacaa tccaggaagt
     2761 tgcctttctc cggtcgagaa cggggaagat gaagttaatg ccgggctcga gtattttatg
     2821 aaactttccg agacgttcca ctatgacacg gcttgcctga ggaacgatct tgatgcccga
     2881 catcaccagg atcacggtaa acagaacgaa gaagatgacg accgttgtgt tgcccgtgtt
     2941 gctgacgaac agaatctctc tcattgtttt cacactcccc gtttatcgga tgtgttcctg
     3001 aaaattattc aagaatgatc tagtcagaat accctgtgca ttactcttca ccggatgact
     3061 cgttctcttc gaactgaacg tccaggctgg ctccccgaac ttttacgacg acgacacccg
     3121 ccccctctcc gatttcgatc ccttcgggac caaacgccca ccaggttttc ccgtccacct
     3181 tcacgtatcc cccttgagca gcgtccggcg agatggtctt tattacgatg ccttttttcc
     3241 cgatgagcgc ctcggcgttc gacgtgcggg catctttcct gttcgacaga aatctgagaa
     3301 cggcaggccg catgaagaaa aagatgaaaa gagtagatac gccgaagaca gcgagttgag
     3361 cacgcagatc aaagtcttca aagtatgtga ttatcgccgg agggatgcag gcgatggcga
     3421 aacatgcaag aacgaagccc ggagtgaaca tctcgacgat cagcaaggca aatcccagaa
     3481 tcacccaaag ctgccagttt gtcacaagct cgaacatttt tctcacctcg actttctcga
     3541 attgaacaac gtaccctctc cgatgaaaaa ggcatcagaa aaacaactct ttcagccccg
     3601 aaaccgagaa tgcgcaccag gcaagcaatc cgcccataag ataatcccag aaacgtccct
     3661 gttctgtcat cagtcttttt tgcgctgcgc ccagaagaga ccagagaaaa accccgttgc
     3721 tgcatgcaag agtcagaaag acgccgcaac caaaaagcca cgttttcgaa gttgtccacg
     3781 gaagaataaa tgttgagagt gttgtcagcc ccagaagaat aactttaggg ttgagaaact
     3841 ggagtacaaa cccatgccgg aagtcaggag catcttcggc tggagcgttt tccagggagt
     3901 ttcgtttggg gaaggcgatc ttccaggcaa gccagagaat gtacaccgtg ccgccggtcc
     3961 tgagcacagg aagaagagta ggcaacaggc gcgcaagaag aaggttgaag atggcgcaga
     4021 aggtcatcag aagaagaagg cccgacatca ttccggcaat caaggggagc gttttgcgca
     4081 atccgtgaat cctcgcggtg ttcattgcga gaactacatt cggtcccgga gtgttgcacg
     4141 ttatgaggat gtaggtgaaa aacgccgtcc actgcacaag cgcacctcca aagatgatgt
     4201 ttggcgcggc agaagcccgc tttgattttc atattataca cgacgaacga ttccagcaga
     4261 gcagcttcac aaacaagtag acttacggtt ttcaccgctt gcattgttga aagtttatgc
     4321 taacatgatg aagcatcatc aagacatctg ttttctcctc tctcaggaaa gcgaaagaac
     4381 agacaccgcg aatcacaatc aaggagcgaa tcaaatgact ttttatgatc gattcagcgt
     4441 ggccgggaaa gttgccgttg ttactggagc agcccgggga cttgggaagg ggtatgcgct
     4501 tggattagcg tctgccggcg cgactgtatt atgcggcgac gtgaacgaaa acgatgcgaa
     4561 attgacggct gacgag
//
LOCUS       DAOSWW010000087         4545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_12935_length_4545_cov_4.416704, whole genome shotgun sequence.
ACCESSION   DAOSWW010000087 DAOSWW010000000
VERSION     DAOSWW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12935_length_4545_cov_4.416704"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PK442_02830"
     CDS             complement(<1..1185)
                     /locus_tag="PK442_02830"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOO86613.1"
                     /translation="MNTEICERVSKELKLPRDSVHAVLLLLEKDCTVPFIARYRKEAT
                     GSLDEEAVLAIKERSIRLEELEARRSAIHASLTDRGLLNPELEKAIANAASLTALEDI
                     YLPYRPKRKTRASIATEKGLAPLADLLLRQEGNDPFSLAISFLSEEKGVATEEEALQG
                     AKDIIAERISENREARRQVRILFGRRGKLKSSCIKGKEEEGANFKDWFAWEEPAALAP
                     SHRILAMFRGEREKVLSLSLLPPEEEALRILRDIFVHGVGPDSILVNEAIIDGYRRLL
                     APSMETELRNALKKKADREAIAVFAKNVREILLASPLGPRNILAVDPGFRTGCKFVCL
                     NRQGDLLFNGTIFPHPPRADEAKSAEIMRKAIDQYGIEAVAIGNGTAGRETERFFRSL
                     ELP"
     gene            1303..1926
                     /locus_tag="PK442_02835"
     CDS             1303..1926
                     /locus_tag="PK442_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO86614.1"
                     /translation="MGRNTWLPLYRRLVLGLSLPVDAEALRGKVALHFSDTPSTFYGV
                     MRRLIRFFEPQCIVHTGDLADDVKLEIRPGELPLYRRKLMELTSVLSSVQKSRIVLVT
                     GNHDHEESVREFFPDSSVIIKSGRIEMCGLDFNLSHEPEGLQTPYGHFNLFGHAPVPE
                     EAPSQGQVFLNGLYAIHVLRVDTGEVLALEYPGYVNDARLNKRRVGL"
     gene            1948..2970
                     /locus_tag="PK442_02840"
     CDS             1948..2970
                     /locus_tag="PK442_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86615.1"
                     /translation="MLSIVRGGPRVLFLKGERSALDHALSAAFSVTRHTFESALDCAG
                     EGQTIVALHGKEDDGGREFRIVSSSSDEVLAALVNDSSCRLESVRLLPRILFFRIFGD
                     KQRVLDQIENDLAGGGIPGATVRRGRLRALLGSSRRNEIAVCFTYAPLNGLIAVADML
                     DDVLFVEGTFFYDLNVFLRSRGLWYFSEGLENRSWNEMEIRIYDSWGFFMEHAERLRA
                     LLGSLEIGMILGEGWGKDFAHILMPVQIYRLRLFTFLSTLLVKELLMGLEYAENGDRL
                     ADFDLYSGRDKISWGSLKDRGAGRANLGKTAREKLFSRLSARDRERLTAMEHRLLRLR
                     QGGNNG"
     gene            3255..3515
                     /locus_tag="PK442_02845"
     CDS             3255..3515
                     /locus_tag="PK442_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOO86616.1"
                     /translation="MEVLKVSAKSQPKSVAGAIAAVLREKGAVEVQAVGAGAVNQSVK
                     SIAIARGYVAPNGIDLVCIPAFSKISIDDEERTAIKFQLESR"
     gene            3728..>4545
                     /gene="hisS"
                     /locus_tag="PK442_02850"
     CDS             3728..>4545
                     /gene="hisS"
                     /locus_tag="PK442_02850"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOO86617.1"
                     /translation="MAEMSAPKGVRDILPEESWKWAYVFRMVQETARHFGYSEVHLPI
                     FEHTELFSRGIGDTTDVVEKEMYTFLDKGDRSITLRPEATASMVRAYLEHRMGTMAQP
                     VKMWCAGPMFRYERPQKGRYRQFWQVDFESIGASSPYADVEIIAISLELFRRLGLRHL
                     EVVINSVGCPECRPDYRKRLTDYFESRKDNLCETCLGRLERNPLRILDCKRDGCKAVT
                     EEAPDVYDSLCGECREHFDVVKDSLDALGASYRLDKRLVRGLDYYTKTAYEILSG"
BASE COUNT         1004 a         1130 c         1297 g         1114 t
ORIGIN      
        1 agggagttca aggctacgga aaaaacgttc tgtttcccgc cccgccgttc cgttgcctat
       61 ggcgacggct tcaattccat actggtctat tgccttccgc atgatctccg cgctcttcgc
      121 ttcatcggca cggggaggat gaggaaaaat agtcccgtta aagagcagat caccctgccg
      181 gttaaggcag acgaacttgc agcctgtccg gaaacccgga tcaactgcga gtatattccg
      241 ggggccgagc ggcgaggcga gaagaatctc ccggacgttc tttgcaaaga cagcgatagc
      301 ctccctgtcc gccttttttt tcagcgcgtt gcggagttcg gtctccatgg agggcgcgag
      361 caggcgccga tagccgtcga taatcgcttc attcacaagt atggaatcag ggcctacgcc
      421 atgcacaaaa atatcccgga gaatacgaag cgcttcttcc tcgggtggga gcagagagag
      481 cgaaagcacc ttctcccgtt ctcccctgaa cattgccagt atcctatggg aaggagcaag
      541 cgccgcgggc tcttcccaag cgaaccagtc cttgaaattg gctccttctt cttcttttcc
      601 cttgatacag ctgcttttca gctttcctct cctgccgaaa agaattcgga cctggcggcg
      661 cgcctcccta ttttcgctga tacgttcggc gataatatcc tttgcgccct gcagagcctc
      721 ttcttctgtt gcaactcctt tttcttcgga gaggaaagag attgcaaggc tgaaaggatc
      781 gttcccctcc tgacggagta gcaggtcggc gagtggagca agtcctttct cagtcgctat
      841 ggacgctctc gtcttgcgtt tcggcctgta tgggagatag atatcttcga gcgcagtaag
      901 agaagctgcg ttcgcgattg ccttttccaa ttcaggatta agaagcccac ggtcagtaag
      961 cgaggcgtga atcgcgcttc tgcgcgcctc cagctcttca agacggatcg aacgctcctt
     1021 gatagcaagg acggcctctt catccaatga gcctgtcgcc tctttcctgt acctggcaat
     1081 gaacggcaca gtacagtctt tttcgaggag caggagcact gcgtgcacgg agtcgcgcgg
     1141 cagtttgagt tcttttgaca ctcgttcgca aatttcggtg ttcatcagga atcctccgat
     1201 cccgcaaatg catgtatgag gcctcttgtc ctttcattct agcacgagaa gaaatgcggg
     1261 gaaatatcgt ggtagaatct gttgtgaaga gagggttgct ttgtgggaag aaatacgtgg
     1321 cttccgctgt acagaagact tgttttgggg ctgtctctcc ccgttgacgc agaagcgctt
     1381 cggggaaaag tagcgctgca cttctcggat actccgtcca ccttttacgg agtgatgagg
     1441 cgtttgatac ggtttttcga gcctcagtgc atcgtccata caggagactt ggcggacgat
     1501 gtgaagcttg agattcgtcc gggcgaactc cctctgtatc gaagaaaact gatggagctc
     1561 acttcggtgc tctcatctgt tcagaagagc aggatagttc ttgttacggg gaatcacgat
     1621 cacgaagaga gtgtccgcga attcttcccc gacagttctg taattattaa gagcgggcga
     1681 atcgagatgt gcggtctgga tttcaatcta agccacgaac cggaaggatt gcagactcca
     1741 tacggccatt tcaacctttt cgggcatgcg cctgttccgg aggaagcgcc gagtcaagga
     1801 caggtgttcc ttaatggcct ttatgcgatt catgtcttac gtgtcgacac aggggaagta
     1861 ttggccctgg agtaccccgg gtatgtgaac gacgcccggc tcaataaaag aagagtcggc
     1921 ctttgattac agtagggagg tgtgttgatg ctttcgattg ttcgaggcgg tcctcgggtt
     1981 ctcttcctta agggagagcg aagcgctttg gatcatgcgc tttccgccgc tttctccgta
     2041 acgaggcata cctttgaaag cgccctggac tgcgccggag aggggcagac gattgtcgcg
     2101 cttcacggaa aggaggatga cggagggaga gagttccgga ttgtttcctc ttcctctgac
     2161 gaggtgcttg ccgcccttgt gaacgacagc tcctgccgcc ttgaatcagt ccgtctgctt
     2221 cctcgtattc tctttttccg tatcttcggc gacaagcagc gtgtgcttga tcagattgaa
     2281 aacgatctcg cagggggagg tattccggga gcaaccgtac ggagagggag gctgcgagct
     2341 cttttaggga gttcacgaag aaacgagatt gcggtctgct ttacgtacgc tcctctgaac
     2401 ggtctcatag ccgtggcgga tatgctggac gacgtgcttt ttgtggaagg gactttcttt
     2461 tatgacttaa atgtgtttct ccggagcagg ggcctctggt atttcagcga agggctggaa
     2521 aaccgctcat ggaacgagat ggaaatacgg atatacgatt catggggttt tttcatggag
     2581 cacgccgagc gtcttcgcgc actcctcggc tctctggaga taggcatgat cctcggagaa
     2641 ggctggggga aggattttgc gcatattctg atgcctgtcc agatataccg ccttcgtctt
     2701 tttacttttc tttcgacgct gctcgtcaag gagttgctca tggggctgga gtacgcggag
     2761 aacggcgaca ggctggcgga ctttgatctg tatagcggac gtgacaaaat ctcatgggga
     2821 agtttaaaag accgtggagc aggacgcgcg aacctgggaa aaacagcccg tgaaaagctt
     2881 ttttcccgcc tttcggcgcg tgaccgcgaa cggttgaccg ctatggagca ccgtctgctt
     2941 cgcctgcggc agggtggaaa caacgggtga aaatgagccg tcgcaagaca ggagtccatc
     3001 tccttgcgcc tcgaattact gaaacaggat gatagagcgt ttttttgaaa aaagagaaaa
     3061 aagcgattca ctccttgcaa agaacttgct cctgcgatta tactaattta gaaactttcg
     3121 gagaactcta gtggcagact cttaggcttt ctcgccctga cgaattgaag gagaacttgt
     3181 ttcttcgagc tcatgattcg ccggatgcgt tccgatatgc gcagatcgtc aatctacgaa
     3241 ggaggtcgct acttatggaa gtgctcaagg tctctgcaaa gtcacagcca aagtcggtcg
     3301 cgggagccat tgcagcggta ctgcgggaga aaggggcggt cgaagttcag gcagtgggcg
     3361 ccggagccgt gaaccagtcg gtgaagtcca ttgcaatcgc aagggggtat gtcgctccca
     3421 acggtattga tcttgtgtgt ataccggcct tttcgaaaat ttccattgac gatgaagaaa
     3481 ggacggccat caagtttcag ctggaatcgc gctgaattca cgcggcgtat tcaacgcatt
     3541 tgagcccggc cccgaattga gggtcgggtt ttgttttttt tagatcatct ctggaaaaaa
     3601 aacgaaggaa tgggtatact cgcttgcata atgtattcgc cctcgaggtg tctccatctt
     3661 ttctttgagg tgaaggaatg caacagcgtt tcggcggggc aaaaaccgcc ggtcgggagg
     3721 agaaaacatg gcggaaatga gcgcaccaaa aggagttcgg gatattcttc cggaggaatc
     3781 gtggaaatgg gcgtacgtct ttaggatggt gcaggagacg gcccgtcatt ttggatattc
     3841 ggaagtgcat cttcctattt ttgaacatac ggagctcttc tcaaggggaa tcggcgatac
     3901 aaccgacgtt gtcgaaaagg agatgtacac ttttctcgat aaaggggaca ggagcattac
     3961 gctccgtccg gaggctacgg cgtccatggt gcgcgcatat cttgaacacc ggatgggaac
     4021 gatggctcag ccggtgaaaa tgtggtgcgc cggcccgatg ttccggtacg aacgccctca
     4081 aaagggacgg tacagacagt tttggcaggt cgatttcgag agtatcggcg cttcttctcc
     4141 ctatgccgat gtcgagatta tcgccatctc cctggaactc ttccggcggc tggggcttcg
     4201 tcaccttgag gtggtgatca attccgtcgg gtgtcccgag tgccgccccg attaccggaa
     4261 gcgtctcacc gactacttcg agtcgagaaa ggacaatctc tgcgagacgt gtctcgggcg
     4321 cctcgaaagg aaccccctgc gcatattgga ctgcaagcgg gacgggtgca aagcggtcac
     4381 tgaagaggct cccgatgtct acgacagcct ctgcggcgaa tgccgcgaac acttcgacgt
     4441 ggtgaaagac agccttgatg ctctcggcgc ctcctaccgt cttgataagc gccttgtccg
     4501 cggactggac tactatacga agacggcgta cgaaattctc tcagg
//
LOCUS       DAOSWW010000088         4502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_13094_length_4502_cov_3.363391, whole genome shotgun sequence.
ACCESSION   DAOSWW010000088 DAOSWW010000000
VERSION     DAOSWW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13094_length_4502_cov_3.363391"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PK442_02855"
     CDS             complement(<1..295)
                     /locus_tag="PK442_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86618.1"
                     /translation="MQFPKNEPVTDRPLVVLLPAAMEQSPRTDVADALRGREFVSGIL
                     RAHGYVPAPLDITPEILQSPERIQHIVRSTKTRCVFNIFEGFGCDSGSEHRFRA"
     gene            complement(300..1364)
                     /locus_tag="PK442_02860"
     CDS             complement(300..1364)
                     /locus_tag="PK442_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOO86619.1"
                     /translation="MNEEYGHEPDVPLQPVARVSVVYNLKKQAGSDEPDDKYEEYDPI
                     STVEAVAGAVGAYGFSVSLCEQDDDFAARLAEQKPQFVANIAEGRGEARGREAQVPCF
                     LESAGIPYWGSDAVSMAVALDKLHTSRTLLCDEIPVPLCACFKQEQDLSQLPALFTER
                     SRFVVKPRYEGSSKGIFSDSVAHTPDEAKEKIRRIWGRYAQPALLEEYLPGDEITVGI
                     VGNGRPSVVGMMRIVPVEQREEFLYSLEEKRDYLKRIRYEGPESIPASVRDQVGRFAL
                     KAFQSLELRDMARVDFRLDSDGTPRIIDINPLPGLSPEYSDLPILHRLSGGEYNDLVR
                     SILRSSFSRNGLAFPARGSR"
     gene            complement(1653..2444)
                     /locus_tag="PK442_02865"
     CDS             complement(1653..2444)
                     /locus_tag="PK442_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOO86620.1"
                     /translation="MDILRRSASLLTPEAWEELDEQAKKVLVANLSARKFVDVEGPKG
                     WTYSAHPTGRLEIDASNSGKSVQFGVNKVLPLVETRATFELDIWELDNISRGAKDPDL
                     SALEKAAKEIALFEEQAIYKGLATAGIEGLATAAKGSSVSLESEEPHGIVNGLSAAVF
                     ALREKAVEGPYAFVASPKLWKAIYSATPGYPTSKHVANLVDKVILSTQDESYVVSLRG
                     GDFELVLGQDLSLGFEERNGGKVRLFFAESFTFRVITPEAIVSLA"
     gene            complement(2486..2833)
                     /locus_tag="PK442_02870"
     CDS             complement(2486..2833)
                     /locus_tag="PK442_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOO86621.1"
                     /translation="MQYTEPADKLDQQTVDQAQALKSLMEEVEAVNYYNQRAALTPNA
                     ELKALLAHNRDEEIEHAAMLIEWLRRNMDGWDEELKTYLFTSAPLTQVESGGGAGAES
                     GVSSKPLGIGSLK"
     gene            3126..3770
                     /locus_tag="PK442_02875"
     CDS             3126..3770
                     /locus_tag="PK442_02875"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HOO86622.1"
                     /translation="MDEQGKKTLLSEDIRELLSLKPGENVLFGELAAAVADRGFGILL
                     VLLSLPSALPVPAPGYSTPFGIVLFILGVQMLLGRETPWIPGWAGRREIKRKTADRMI
                     SGAAKFFSFVEIFIRPRFEFVSGKGAGMICSLLLLLMSGLMILPIPLTNTLPAMVIFC
                     IGVALTERDGLAFIAALLFGAAATALYTLAVWVIFYLGAQGIGEAKEIIKGWFF"
     gene            3849..>4502
                     /locus_tag="PK442_02880"
     CDS             3849..>4502
                     /locus_tag="PK442_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO86623.1"
                     /translation="MSIRRKMLVLIAGFLLVTLVLTCGSYVLTGRSVRVQVEDTGRTM
                     VAEIASAMDEYVAKLLSLTGSLAVSLSYISEEGDPDYVELFGRYLEAAQTHGVQTVFM
                     GFESKEFADSTAWEPPEGYDPRTRSWYVETLKKNGVTVTSPYVDLITGELIVSVTAPV
                     RSQSGMLLGVAGLDVSLEHVRRKVLTRRLFGEGFPFLVDRHGFFLAAPFEDWTLAENI
                     "
BASE COUNT          908 a         1185 c         1332 g         1077 t
ORIGIN      
        1 gcgcgcggaa gcggtgttcg gaaccgctgt cgcagccgaa cccctcgaag atgttgaaga
       61 cgcagcgcgt cttggtcgag cggacaatat gttggatgcg ctccggagat tggaggatct
      121 cgggcgtgat atcgagaggc gccgggacgt atccatgagc ccgaagaatg ccggagacga
      181 attcacgccc ccggagcgca tcggcgacgt ccgtgcgcgg agactgttcc atcgcggccg
      241 gaagcaggac gacgagaggc ctgtcggtca caggctcgtt tttcgggaac tgcattcgat
      301 caccggcttc ccctggcggg gaaggccagg ccgttcctgg agaaggagct tctcagaatg
      361 gaacggacaa gatcgttgta ctccccgccg ctgagacggt ggaggatcgg aaggtcgctg
      421 tattccgggg agagccccgg aagcggattt atgtcgatga ttcttggagt tccgtcgctg
      481 tccagccgga agtccacccg cgccatgtcc cgcagttcca gcgattgaaa cgccttcagc
      541 gcgaagcggc cgacctggtc gcggacggaa gcggggatgc tctcggggcc ttcgtagcgg
      601 attcgcttca agtagtcgcg tttctcttcg agagagtaga ggaactcttc ccgctgttcg
      661 actggaacga tgcgcatcat ccccaccacg gaggggcggc cgttgccgac aatcccgacc
      721 gtgatctcat ctcccggaag atattcttcc aacagcgccg gctgcgcata ccggccccat
      781 attctgcgga ttttctcttt tgcctcgtcg ggcgtatgcg cgacggaatc cgaaaagatt
      841 cccttggaag acccttcgta cctcggcttc acgacgaagc gggaacgctc ggtgaagagc
      901 gcggggagtt gcgataggtc ctgttcctgt ttgaaacagg cgcacagagg gacggggatc
      961 tcgtcgcaga gaagcgtcct gctggtgtgc agcttgtcga gcgccaccgc catggaaacg
     1021 gcgtccgatc cccaatacgg gatgcccgcg ctctccagaa aacagggaac ctgcgcctcc
     1081 cgcccccttg cctccccacg tccttcggcg atattggcga cgaactgagg tttctgttca
     1141 gcaagacgcg ctgcgaaatc atcgtcctgc tcgcagaggg agactgagaa tccgtatgcc
     1201 ccaacagcac cggcgactgc ctcgacagtc gaaatcgggt cgtactcctc gtacttgtcg
     1261 tccggctcgt cggagccggc ttgttttttg agattataga cgacggaaac tctggcgaca
     1321 ggttgcaacg gaacgtcggg ctcgtgcccg tactcttcgt tcatagattt tcccctcctg
     1381 aaggatacat gaatagagac ctctggaaac gcggtcgatg cggttttcca tttcttcgaa
     1441 tccgatgttc gggaaccgcc atgtctccct cctccaagag tttgccccct ccgtgcgaag
     1501 agggtctttc agtgcggatt atagaggttt ttgggggaaa tacaagaggg aaattccgtt
     1561 gcgggatagc tttttcgcag gctgtgcgac gcaaaaaaaa aggggctctg gagagcccct
     1621 gcgtcaggtg ttttcaaggg agtcaacaac ggttaggcaa gcgaaacgat ggcttcgggg
     1681 gttataactc tgaatgtgaa tgactcggcg aagaaaagcc gcacctttcc gccgttccgc
     1741 tcctcgaagc cgagtgaaag atcttgtccg agaacgagtt caaagtcgcc gccgcggagc
     1801 gagacaacgt acgactcgtc ctgggtggag aggatgacct tgtcgacgag attggcgacg
     1861 tgttttgatg tcggataccc cggtgttgcg ctgtatatgg ctttccagag tttcggagat
     1921 gcaacgaatg cgtatggacc ttcgactgcc ttctcccgga gagcgaacac tgccgctgaa
     1981 agaccgttga cgattccgtg cggctcctcg ctctcaagag aaacggagct tcctttcgca
     2041 gcggtcgcaa gaccctcaat acctgccgta gccaagccct tgtaaattgc ctgttcctcg
     2101 aaaagggcga tctccttcgc ggccttttca agcgcggaca ggtcggggtc tttggctcct
     2161 ctgctgatgt tgtcgagctc ccaaatatcc agttcaaacg ttgcccgggt ctccaccaga
     2221 ggaagcacct tgttgacgcc gaactggacg ctttttccgg agtttgaggc gtcgatctcc
     2281 agccttcctg tcgggtgtgc gctgtatgtc caccccttgg ggccttcgac gtcgacgaac
     2341 tttcgagccg aaaggttcgc aacgagtact tttttcgcct gttcatcaag ctcctcccaa
     2401 gcctccgggg tcaggagcga tgcggatctt ctcagaatat ccatgagtgt gtacctcctt
     2461 tacatgttcg tctgctctgc aggaactact tgaggctccc gatgccgagg ggcttggaag
     2521 aaacgccgga ttcggcgcct gctcctcccc cggattcaac ctgagtgaga ggggccgagg
     2581 tgaagagata tgttttcagt tcctcatccc atccgtccat atttctccga agccattcga
     2641 tgagcattgc ggcgtgttct atctcttcgt cgcggttatg agcgaggagc gctttaagct
     2701 ccgcgttcgg cgtgagtgca gctcgctggt tgtagtagtt taccgcttct acttcctcca
     2761 tgagactttt gagcgcctgc gcttgatcaa ctgtctgctg atcaagctta tctgcgggtt
     2821 cagtgtattg catggtatct ctcctttctg gaaatcacga atagatgatg ccgaccgtgc
     2881 ggactcggca tttattccaa cattataccg tctgcagagg aaaatcactc gtgtaaaaaa
     2941 gagacggctt taaataaatc tattacaagg tagtttatat tttatgtgag tatataccaa
     3001 cttctaactt tcgtcaagtt tggtcaaatg gaagaatcgc ttcactgtat gatatcatgc
     3061 ggtgcgaagt gtgggggatg tccgctgaaa agcgtatcat gacgagggga aggtttgtgt
     3121 acattatgga tgaacagggg aagaaaacgc tgttatcgga agatattcga gagctgctct
     3181 cgttgaaacc tggagagaac gtcttgttcg gagaacttgc agcagctgta gctgatcgtg
     3241 gattcggcat ccttctggtg ctcctttcgc ttccgagcgc gcttcccgtg ccggcgccgg
     3301 gatacagcac gccgtttgga atagtgctct ttatcctggg tgtgcagatg ctgctgggca
     3361 gagagacgcc gtggattccg ggatgggcgg gacgaaggga gatcaagcgg aagacggcgg
     3421 acaggatgat ctcgggtgcg gcaaagtttt tttccttcgt ggagattttc atacgccccc
     3481 gtttcgagtt cgtctcggga aagggcgcag gaatgatctg ttctttgctg ctgctgctta
     3541 tgagcggact catgattctt ccgattccac tgacgaacac gctccccgcc atggtgatct
     3601 tctgtatcgg cgtggcgctc acagagcgcg acgggctcgc gttcatcgcg gcgctgcttt
     3661 tcggcgcggc tgctacggcg ctgtatactc tggcagtctg ggtaattttt tacctcgggg
     3721 cccagggaat aggcgaggcg aaggagataa tcaagggctg gtttttctga tcccgtactt
     3781 ttctgtacac gtcatgattc gccaatgttt ttcagggttt ccccggaaac gagaggggtg
     3841 atactgctgt gtctattcga cggaagatgc tggtgttgat cgccgggttt cttcttgtaa
     3901 ctttggtatt gacttgcggc agctacgtcc tcaccggaag gagcgtgcgc gtccaggtcg
     3961 aagataccgg gcgaactatg gttgcggaga ttgcctccgc tatggatgaa tatgtagcga
     4021 aacttctctc ccttactggc tcgctggctg tttctctctc atacatcagc gaggagggcg
     4081 atcctgacta tgtagaactt ttcggccgct atctggaggc tgcgcagacg cacggagtgc
     4141 aaacggtctt catgggcttt gaatcgaagg agttcgccga ttctaccgca tgggagccgc
     4201 cggaagggta cgacccccgg acgcgctcgt ggtatgtgga gacgctgaag aaaaacgggg
     4261 tgacggtcac ctctccatat gtcgacctga taaccggaga gctcatcgtg agcgtcacgg
     4321 cgcctgtacg aagccagtcg gggatgttgc tcggtgtggc ggggctggat gtgagcttgg
     4381 agcacgtgcg aagaaaagtg ctcaccagaa ggctgttcgg agaaggtttt ccattccttg
     4441 tcgacagaca cggttttttc cttgcagctc ccttcgaaga ctggacgctt gcggaaaaca
     4501 tc
//
LOCUS       DAOSWW010000089         4479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_13191_length_4479_cov_3.646248, whole genome shotgun sequence.
ACCESSION   DAOSWW010000089 DAOSWW010000000
VERSION     DAOSWW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13191_length_4479_cov_3.646248"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="PK442_02885"
     CDS             <1..1126
                     /locus_tag="PK442_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOO86624.1"
                     /translation="SVDSFWGLIIGVLVIVASFASIRFLGEGIIDEFFDPLFEAWLLP
                     VAERLSLFLGGEGLFHDIVVGRLIDGQIDFEQSFGLLTTGLYVPLAMVLPYVAAFYFV
                     LSLLEDFGYLPRLAVIFDALLHRMGLHGFAIVPSLLGLGCNVPGILATRVLDSDRERF
                     IAATLISVAIPCAGLQAMILGTVGQLGMRWVFAVYATLILSWLILGRLLHSILPGFSP
                     ELIVEIPPYRIPSFKSLGLKLWIRIRGFLLEAVPLVLGGILVVGLLDSTGVTAFLSRL
                     LAPVFNGLLGLPAEASGPILLGILRKDVAMGMLGALGLSPVQLVVATVTLSMTFPCIA
                     TFIVLWKELGGRRMAASMGIMVFSALVAGTAVRLLLSIHF"
     gene            complement(1196..1639)
                     /gene="aroQ"
                     /locus_tag="PK442_02890"
     CDS             complement(1196..1639)
                     /gene="aroQ"
                     /locus_tag="PK442_02890"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOO86625.1"
                     /translation="MKRILMLHGVNHNMFGKRDPAQYGTITLDEIDEKLRTLGAELGV
                     EVVGFQTNSEGAMCERIHQAFQEGMDGVIINAGAWTHYSYAIRDALAILTVPVAEIHM
                     SNIHAREEFRHRSVFAEVVKGQICGFGLDSYLLGLRAVVSAIGGA"
     gene            complement(1720..2517)
                     /locus_tag="PK442_02895"
     CDS             complement(1720..2517)
                     /locus_tag="PK442_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOO86626.1"
                     /translation="MQGHTTDEKTTVTGTGEEKSVRSVERAFSILMCFTLDEDELTLM
                     DIARKMHLPTTTVSRMVNTLVGLGFLEKSPGKTYSLGANLYYLGAVARNHFKLQKIVY
                     PYMKQVRDTTKEAVSLYVLDGDARVCYEHVESLLSMRCVVRVGDRFPLWAGASGKCLL
                     AYADPELVDRELAKAKPITPTTIISREAFLADLETIRKKGHAISYGEREEGITSIAVP
                     VFHPKNKVTTVFSVAGPSVRFTDEIIEELLPVVKAMTEEISRSLSYR"
     gene            complement(2562..3455)
                     /gene="aroE"
                     /locus_tag="PK442_02900"
     CDS             complement(2562..3455)
                     /gene="aroE"
                     /locus_tag="PK442_02900"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOO86627.1"
                     /translation="MFNAKVNTRFFGLLGNPVGQSAAPFMHNSVYQKLGLDMLYVPLE
                     LEMESLETVVRTMQLFNYAGTGVTMPFKTQVHKYVDGLADSARYTEVVNTIVIEKDGR
                     KIGHNTDGTGFVESLEKQAGLNIPDHRYLLLGSGGAATAIACALAMRGATAMRSLCIR
                     QDYAYAETLYTRVNAHFPSVCEIDEMNDTTIRKGLEEFDVIIHATKVGLYPKTGDILF
                     DPALLSSSHLVCDVVYVPMETKLLVEAEKRGCRTLSGLWMNVYQAAEQMRLWAGIEEP
                     PIDFMYQCGIEYLRREGKLTA"
     gene            complement(3478..4305)
                     /locus_tag="PK442_02905"
     CDS             complement(3478..4305)
                     /locus_tag="PK442_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOO86628.1"
                     /translation="MQPQFSLAQLTVLKTSPAEISRIAADCGYDYVSMRQIYMGLPEE
                     PDYDLSKNKKLMAETKAVFADTGIKLLDIELARIFDGMDVKKYEPAMTTAVELGGKHV
                     LSSIWTLDREYAIERFAELCDLAAQYGLTVELEYVPIAGVRTLGGAVDVLRKVNRKNA
                     GLMIDIHHFHRAQDDPAELAKLPEEWFRFAHLCDAPAEIPSEQTEMVRILREARSYVG
                     EGGIDIAPILNAMPVVPYSIELPNLERVAESGYAEHARRCLESAKAFCAGHLKGRSE"
BASE COUNT          824 a         1409 c         1189 g         1057 t
ORIGIN      
        1 ttccgtggac tctttttggg gactgattat aggagttctc gtcatcgtcg cgagctttgc
       61 ctccattcgc ttccttggag agggaatcat cgacgaattt ttcgacccgc tctttgaagc
      121 gtggctgctc cctgtagccg agcgtctttc cttgtttctg ggaggggaag ggcttttcca
      181 tgacatcgtc gtcggccgcc tcatcgacgg gcagatcgac ttcgagcaga gcttcggcct
      241 gctcaccacc ggtttatacg tccccctggc gatggtcctg ccgtatgtcg cagcgtttta
      301 cttcgtcctc agtctcctcg aggacttcgg ttacctcccg cgcctcgccg tcatcttcga
      361 cgcccttctt caccgaatgg gcctccacgg cttcgccatc gtcccctcgt tgctcgggct
      421 tggctgcaac gtcccgggta tcctcgccac cagggtgctg gactccgacc gggagcggtt
      481 catcgccgcg acccttatct cggtcgcaat accctgcgcc ggacttcagg cgatgatact
      541 gggaacggtc ggacagctcg gcatgcggtg ggtttttgcc gtctatgcaa cgctcatcct
      601 ttcgtggctc atacttgggc gcctgttgca ctccattctc cccggattca gtccggaact
      661 tatcgtggag atcccaccct accgcatccc gtcgttcaaa tcgcttggac tgaagctctg
      721 gatacgaatc cgcggatttc tcctcgaagc tgtgccgctt gtccttggag gcattctcgt
      781 cgttggtctc ctcgactcga caggcgttac cgcatttctt tcgagacttc ttgcccctgt
      841 cttcaacggc ctgctcggtc tcccggccga ggcgtccggt cccattctcc tcggtatcct
      901 gcgaaaggac gtggcaatgg ggatgctcgg cgcgctcgga ctctcgccgg tacagcttgt
      961 cgtcgccaca gtgacgctgt cgatgacctt cccgtgcatc gccaccttca tcgttctctg
     1021 gaaggagctc ggcgggcgta gaatggcggc ctccatgggg atcatggttt tctccgctct
     1081 tgtcgccgga acagctgttc gcctgttgct ctcgattcac ttctgaagcg agtcaaataa
     1141 gagggagtgg gaggggcgaa gtcgcccctc ccactccctc tcgaagtgtt gtttgctacg
     1201 cgccgcctat cgcgctgacg accgcgcgca ggcccaacag atagctgtcg aggccgaaac
     1261 cgcagatctg cccctttacg acctccgcaa agaccgaacg atgccggaat tcctccctgg
     1321 cgtgtatatt cgacatgtgt atctccgcca cgggaacagt caggatcgcc aaggcgtccc
     1381 gaatcgcgta gctgtagtgc gtccatgccc ccgcattgat aatgacgccg tccatgccct
     1441 cctgaaaggc ctgatgaatc cgttcgcaca tcgcgccttc ggaattcgtc tgaaagccga
     1501 caacctcgac gccaagctcg gcgcccagcg tgcgcagctt ctcgtcgatc tcgtcgagcg
     1561 ttatcgttcc gtactgcgcc ggatcgcgct ttccgaacat attatgattc acaccgtgaa
     1621 gcatgagtat tctcttcatg gccctgtttc catctccctt tcgttgttcg agtcgactga
     1681 acgttttcta tgatggagcc caacgaagca aataactcgt tacctgtagg aaaggctcct
     1741 tgaaatctcc tccgtcatcg ccttcaccac aggaagcagc tcttcgataa tttcgtcggt
     1801 aaagcgaacc gacggaccgg cgacggagaa cacggttgtc actttgtttt tcgggtggaa
     1861 cacaggaacg gcgatggagg taattccctc ctcgcgttcc ccgtagctga ttgcgtgtcc
     1921 ttttttgcgg atcgtctcaa ggtcggccag gaacgcctcc ctcgatatta tcgtggtcgg
     1981 cgttatcggc ttcgccttcg cgagttcgcg gtccacaagt tcagggtcgg cgtacgccaa
     2041 gaggcacttg cccgaagctc ccgcccacag aggaaaacgg tctccgacac ggaccacgca
     2101 acgcatgctg agaagactct ccacatgttc gtagcacacc cgagcatccc cgtcgagaac
     2161 gtagagcgac accgcctcct ttgtcgtatc gcgaacctgc ttcatatatg ggtagactat
     2221 cttctgcagt ttaaaatggt tccgtgcgac agcccccaaa tagtacagat tcgctccgag
     2281 cgagtaggtc ttcccgggac ttttttcgag gaagccgaga cctactaggg tgttcaccat
     2341 tcgagagact gtcgtcgtcg gaaggtgcat cttccgtgcg atatccatca gcgtcagctc
     2401 gtcctcgtcg agagtgaagc acatcaggat ggaaaacgcc cgttccactg aacggacaga
     2461 cttttcctcc ccggttccgg tcacggtcgt tttttcgtct gttgtatgcc cttgcatgac
     2521 atccccctct agaacgcccc gctcccactc tcgtgggaag ctcaggctgt cagtttacct
     2581 tctcttcgaa gatattcaat accgcactgg tacatgaagt caataggagg ctcttcaata
     2641 cccgcccaga gacgcatctg ctccgccgcc tggtagacgt tcatccacag gccggagagg
     2701 gtgcggcatc cgcgcttctc cgcctctacg agaagttttg tctccatggg gacatagacc
     2761 acgtcgcaca cgagatgtga cgaactcaga agcgccggat cgaagaggat gtcgccggtc
     2821 ttcgggtaga gcccgacttt tgtcgcgtgg atgatgacgt cgaactcctc cagccccttg
     2881 cgaatcgttg tgtcgttcat ctcgtcgatc tcgcagacgc tagggaaatg agcgttcacc
     2941 cttgtgtaga gcgtctccgc ataggcgtag tcctgccgga tgcagagcga acgcatcgcc
     3001 gtcgcccctc tcatcgcgag agcgcaggcg atcgcggtcg cggcgcctcc cgagccgagg
     3061 agaagatacc ggtgatccgg gatgttcagc ccggcctgtt tctcaagcga ctcgacaaaa
     3121 cccgttccgt cagtattatg accgattttt cgtccgtctt tctctatgac gatggtgttg
     3181 accacctcgg tgtaccttgc ggagtcggcc agtccgtcga cgtacttgtg gacctgtgtc
     3241 ttgaagggca tcgtaacccc cgtgcccgca taattgaaga gctgcatggt gcgaaccact
     3301 gtttcaagac tctccatctc cagctcaagt ggaacgtaga gcatgtcgag tcccagtttc
     3361 tggtagacgc tgttatgcat aaagggcgcg gcgctctgtc ccaccgggtt gcccagaaga
     3421 ccgaagaatc gcgtgttcac cttcgcgttg aacatattct cgaactcctt tcccggccta
     3481 ctctgaacgg cccttgagat gtccggcgca gaaggctttc gccgactcca gacagcggcg
     3541 tgcatgctca gcgtaccccg actccgccac gcgctccagg ttcggcagct cgatcgagta
     3601 ggggacgacg ggcatcgcgt tcagaatcgg ggcgatgtcg atccccccct cccccacata
     3661 cgatctggcc tccctgagaa tccgtaccat ttccgtctgt tccgagggga tttccgcggg
     3721 cgcgtcgcaa agatgcgcaa agcggaacca ctcctccggc agcttcgcaa gctccgcagg
     3781 gtcgtcctgg gctctgtgga agtgatgaat atcgatcatc agcccggcgt tcttccggtt
     3841 gaccttccgg agcacatcga ctgcgccgcc cagcgtcctg accccggcga tgggaacata
     3901 ctccagctcg acggtcaggc cgtactgcgc ggcgagatcg cacaactccg cgaagcgctc
     3961 tatggcatac tcccggtcga gcgtccagat gctgctcagg acatgcttgc cccccaactc
     4021 gacagccgtc gtcattgcgg gttcgtactt tttgacgtcc atgccgtcga atatcctcgc
     4081 gagttcgatg tcaagcagct tgatacccgt atctgcgaag acggccttcg tctccgccat
     4141 cagtttttta ttcttcgaga gatcgtaatc gggctcctcg gggagcccca tatatatctg
     4201 ccgcatgctc acatagtcgt atccgcaatc cgctgcaatc cgcgagatct cggcaggcga
     4261 cgtcttcagc accgtaagct gcgccaggga aaattgaggc tgcataatcc gtactctcct
     4321 cctccgaatg taaaaaaacg ttctcgaacg acggcattcg tcatagggag cgccccttgc
     4381 cggggcgctc cgccttctct cgttacggcc tgggaatacc gaattcctcc ggagagtgct
     4441 tcgctccgcc tgcatcgtac agaagccatg cgctgaccg
//
LOCUS       DAOSWW010000090         4388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_13606_length_4388_cov_3.768290, whole genome shotgun sequence.
ACCESSION   DAOSWW010000090 DAOSWW010000000
VERSION     DAOSWW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13606_length_4388_cov_3.768290"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1103)
                     /gene="aroE"
                     /locus_tag="PK442_02910"
     CDS             complement(201..1103)
                     /gene="aroE"
                     /locus_tag="PK442_02910"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOO86629.1"
                     /translation="MKNETALQQSHALGTVEIRKNVELLVGLLGDPVAHTLSPAMQNA
                     AFQALGAGYRYLAFRVRAEDFEHAMEGLAALGAVGTNITVPHKERAFRWARRLDPSAS
                     RTGAVNTVLFSEEGGTGYNTDVAGIRTALAALSPRRDGALILGAGGAARAAVCAMQDE
                     GFSHIAIMNRTRERAEKLQAQLFPAKSCKLPTCEILSFEEAPSFTPDLLVNTTSLGMH
                     NTPWPEELLDTIVSLAKGGVVLDLVYARGRETHLCDRAQQSGIPSVSGQEVLLHQGTA
                     AFKLFTGMAAPVEIMRAALQKGNS"
     gene            1523..2227
                     /locus_tag="PK442_02915"
     CDS             1523..2227
                     /locus_tag="PK442_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOO86630.1"
                     /translation="MKNDTPVSSGNREFLEQYIPLLDFFAEVCGPEYELVLHDVSRPE
                     SSIVAIRNGQISGRTVGSTMSEYAPTFIKLLQSGGYAEDMVAHVDRTKDNRILESHTF
                     FIKDEKGELRGMICANHDVTSLIKLHDTLHEKIRMLNGLSKDLAERTNEDPPFEAILE
                     KESHSNLNGLMDVLIEQASSEFSVAPPQMSPEERIRFVSMLKTRHLFSMKGAVAKVAE
                     RLGVSEATVYRYLKKA"
     assembly_gap    2280..2379
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2460..4217
                     /gene="iorA"
                     /locus_tag="PK442_02920"
     CDS             2460..4217
                     /gene="iorA"
                     /locus_tag="PK442_02920"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOO86631.1"
                     /translation="MTGNEAIARGAWEAGCHVAAAYPGTPSTEILENVSRYKEIYSEW
                     STNEKVSLEVVSGASMAGARAMCAMKHVGLNVAADPFFTMSYIGVNGGLVIVDADDPG
                     LFSSQNEQDNRWYALHAKLPMLEPSDSQECLDFMKAAFDISERFDTPVLLRVTTRICH
                     SKSVVETGERMERLIREYTRNPAKNAMLPVFAKAKHYLVEKRLLDLREFNETSPFISY
                     VEGDKEIGVLTSGVGYQHSREALGDRVSYLKLGMTWPLPEKTIRDFASKVKKLWIVEE
                     NDAFIEGAVRGMGIACSGKDALPLVDELSPALISKAILGTKPSSGPDTGIAPPNRPPL
                     LCAGCPHRGLLFAMSKKKDLVVMGDIGCYGLGSMPPLNVGESTICMGAGFSAVVGFQK
                     AQEKAGRSQKVFGILGDSTFFHSGITGLIDAVVNKSRGVFVILDNRITAMTGQQENPG
                     TGRTLMGEETTALDIPRLCAACGVKEDHIHVVDPYNLKACRDAVDVAYAAEETSVIVT
                     TRPCALIKEVQKSRASLHCVVDAEKCVLCSSCLKLGCPAITKQEGRIVIDSVACNGCS
                     LCVQVCPKQAISREGELHE"
     gene            4210..>4388
                     /locus_tag="PK442_02925"
     CDS             4210..>4388
                     /locus_tag="PK442_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO86632.1"
                     /translation="MNKNGKNVLLVGVGGQGTILASKLLSEGLLALGYDVKMSEIHGM
                     AQRGGSVSTHVRYGA"
BASE COUNT          951 a         1129 c         1252 g          956 t
ORIGIN      
        1 ctccttttgt gataacaaaa gacgagatag aaaacttttt tgaggccttc gaaaacgtgc
       61 ttgccgagta gatgcttcag cgcgcgttct gttgcacctt gcgtgagaca tgctctcttg
      121 gtaacagttt gtcgcgctgg aggcgatctc cgttcgtgag tgcggggcat tcgtaaccgc
      181 ctcgcaccct tcactgagct ttatgaattc cctttttgca gggctgccct cataatctcc
      241 actggagcgg ccattccggt gaacaacttg aaggctgccg ttccttgatg aagcagtact
      301 tcctgaccgg agacggacgg tatgccagac tgttgcgcgc gatcgcagag atgtgtttca
      361 cgaccgcgtg catacacgag atcgaggacg acgcctccct tcgccaggga aacgattgtg
      421 tcgagcagct cctccggcca gggagtattg tgcattccga gcgacgtcgt gttcacaagc
      481 agatcgggag tgaaagaggg agcttcttca aacgagagta tttcgcatgt cgggagtttg
      541 cagctcttcg cggggaagag ctgcgcctga agcttttcag cccgttcacg tgtgcggttc
      601 ataatcgcga tatgtgagaa tccttcgtct tgcatcgcgc agacggccgc gcgcgccgcg
      661 cccccggcgc cgaggatgag agcgccgtct cttcggggag agagtgcggc aagcgccgta
      721 cggatgcccg cgacgtctgt gttgtagcct gtgccgccct cttcggaaaa aagcaccgtg
      781 ttgacggctc ccgtcctgga tgcggaggga tccaagcggc gcgcccagcg gaatgcgcgt
      841 tccttgtgag gtacggtgat gttcgtcccc acggccccaa gagcggccag tccctccatc
      901 gcgtgttcga agtcctctgc acgaacccgg aatgcaagat agcggtaccc tgcgccgaga
      961 gcctgaaatg ccgcattctg catggcaggc gaaagggtat gggcgactgg gtctccaagc
     1021 agcccgacaa gaagctcaac gttttttcgg atttcaacgg ttcccagcgc gtgtgattgc
     1081 tggagtgcgg tctcattctt catggctttc tcctccttgg cctacgggcc ggacgtatac
     1141 taaaaaagcc ggaacccgaa accgggcccg gccttttgtt ttttctctgt cgacggtttt
     1201 gcgctacatg actccgtgga cgctctggct gcgggagaac cggtttccat acgaatgctg
     1261 accgtcggcg taataatacc aacggtcgta gctaaaggaa aaaccggaaa ctccgcgggg
     1321 tgaaagacgt ctcaagatca tgaaaacctg ctttctccca tcaagtgtgt ctgaaagttg
     1381 cgggtaataa tacccccctt tcagtgaggt gtcaatacac gcccgctcaa ctcggccgta
     1441 aaacttcttt acagaatccg caggacgtga tatgaatttt ttatcacaac gttgtgtatt
     1501 ttttgaaggg aaggaacgct tcatgaaaaa tgatacgccg gtctcttcgg ggaaccgtga
     1561 gtttcttgag cagtatatac cccttctgga ttttttcgca gaagtgtgcg gtccggagta
     1621 cgaactcgtg ctgcacgatg tgtcgcgtcc cgagtcgtcc attgtagcca tccggaatgg
     1681 ccagataagc ggaagaacag tggggagcac gatgtcggag tacgcgccga catttatcaa
     1741 actcctccaa agcggcggat acgcggagga tatggtggcc catgtcgaca ggacgaagga
     1801 taatcgaatc cttgagtctc ataccttttt tataaaggat gagaaggggg agctgcgcgg
     1861 catgatatgc gcaaatcatg atgtgaccag ccttataaaa cttcacgaca cgctccacga
     1921 gaaaatcagg atgctgaacg gactttcaaa agatcttgcg gagcggacca acgaggatcc
     1981 gccttttgaa gcgatcctgg agaaagaatc tcattccaac ctgaacggcc tgatggatgt
     2041 gcttatcgaa caggcctcat cggaattttc cgttgctcct ccccagatga gcccggaaga
     2101 gagaattcga tttgtgagca tgctcaaaac gcgccacctc ttctcgatga aaggggcggt
     2161 ggcaaaggtg gcggaacgtc ttggcgtttc tgaagcgacg gtttatcggt atctgaagaa
     2221 agcgtgagca ggctctttat tgaaggctgt gtaaagagcg ttttggagta gcgtctctcn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tattatcaca ccgtcgcttc
     2401 ttcttcgtga agaaggcggt ctgttcggaa ggagtggagc gaagaagatg agagaactga
     2461 tgaccggaaa cgaggccatt gccagagggg cctgggaagc ggggtgccac gtggcggccg
     2521 cctatccggg gaccccgagc accgaaatcc tcgaaaatgt ctcccgctac aaggagatct
     2581 attccgagtg gtccacaaac gaaaaagttt ctctggaggt cgtctccgga gcgtcaatgg
     2641 cgggggcccg ggcgatgtgc gccatgaagc acgtgggatt gaacgtggcg gcggatcctt
     2701 ttttcacgat gtcgtatatc ggcgtcaacg gaggtctcgt catcgtcgac gcggacgacc
     2761 ccggactttt cagctcgcag aacgaacagg acaaccgctg gtatgccctc catgcaaagc
     2821 tccctatgct cgaaccttcg gacagccagg aatgtcttga tttcatgaag gccgcctttg
     2881 atatctctga acgcttcgac actccggttc tccttcgcgt cacgacgcgt atctgccaca
     2941 gcaagagcgt cgtcgagaca ggcgaacgta tggaacgact cattcgtgag tatacccgca
     3001 atccggcgaa gaatgcgatg cttcctgtct ttgcgaaggc gaagcactat ctcgtcgaaa
     3061 aacgtcttct tgatctccgt gagtttaacg agacgtcgcc gttcatctcg tacgtggagg
     3121 gcgataagga aataggcgta ctcacgagcg gcgtcggtta tcaacattcg agagaggcgc
     3181 ttggcgacag ggtgagctat ctgaaactgg gtatgacctg gcccctgcct gagaaaacca
     3241 tccgggattt tgcttcgaag gtgaagaaac tttggattgt tgaggaaaac gacgcattca
     3301 tcgagggggc agtaaggggc atgggtatcg cctgttccgg gaaggatgcg ctccctcttg
     3361 tggatgaact ctctccggct ctgatatcga aagcaattct cggaacaaag ccctcctccg
     3421 gacccgatac cggaattgcg ccgccgaacc gtcctccgct cctctgcgcc ggatgcccgc
     3481 accgtgggct tctcttcgca atgagcaaga agaaggatct ggttgtgatg ggagatatcg
     3541 gctgctatgg acttgggtcg atgcctccgc tcaacgtagg cgagagcacc atttgcatgg
     3601 gagcggggtt ttcagcagtc gtcggtttcc agaaagcgca ggaaaaggcc ggccgctccc
     3661 agaaagtctt cggaatcctc ggtgattcca cttttttcca ttccggtatt acgggcctta
     3721 tcgacgctgt ggtgaacaag tcgaggggtg tgttcgtcat ccttgacaac cggattaccg
     3781 cgatgaccgg acagcaggag aaccccggca cgggaaggac gctcatgggc gaggagacga
     3841 ccgctttgga catcccgcgt ctctgcgccg cgtgcggcgt caaggaggac catatccacg
     3901 tcgtcgatcc atataacctg aaggcgtgcc gcgatgcagt ggacgtagcg tacgccgcag
     3961 aagaaacatc ggtgatcgtg acgacccgtc cctgcgcttt gatcaaagag gttcagaaaa
     4021 gcagggcctc gctccactgc gtcgtcgatg cggagaagtg cgttctctgc tcttcgtgcc
     4081 tcaaactcgg ctgtccggcg atcacgaaac aggaaggccg gattgtcatc gactccgtcg
     4141 cctgcaacgg gtgttctttg tgcgtgcagg tctgcccgaa acaggctatt tcgagggaag
     4201 gtgagcttca tgaataagaa cggaaagaac gtccttctgg tcggcgtagg cggtcaggga
     4261 acgattctcg cgagcaagct cctctcggaa ggacttcttg cactcgggta tgacgtgaaa
     4321 atgtccgaga tccacgggat ggcgcaacga ggcggcagcg tctccacgca tgtccgctac
     4381 ggcgccag
//
LOCUS       DAOSWW010000091         4380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_13642_length_4380_cov_3.313295, whole genome shotgun sequence.
ACCESSION   DAOSWW010000091 DAOSWW010000000
VERSION     DAOSWW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13642_length_4380_cov_3.313295"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK442_02930"
     CDS             <1..433
                     /locus_tag="PK442_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86633.1"
                     /translation="AELSSSLQFQVREAGAALRVGRLPSCRGDAVQLTQVFANLLGNA
                     LKYLDPQRPGEIVVEGRIEQDRAVYLVRDNGIGIAPQHQEKIFQLFHRLNPSANEGEG
                     LGLTIVRQILGRLDGEVVVESTPGTGSTFTITLPAASKKGE"
     gene            454..900
                     /locus_tag="PK442_02935"
     CDS             454..900
                     /locus_tag="PK442_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86634.1"
                     /translation="MHNEVIILIAEDDEGHAGLIRKNLARAGISNHVEHFKDGQEIID
                     FLFRIGNGPHRRSHTPYILLLDIRMPKLDGTEVLKRIKEDHELRKMPVIMITTTDDPR
                     EVERCHELGCSSYITKPVEYDAFVNAVRQLGLYLAVVQVPQVNGVH"
     gene            921..>1431
                     /locus_tag="PK442_02940"
     CDS             921..>1431
                     /locus_tag="PK442_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86635.1"
                     /translation="MDGRAEHGDAPARNVLVVEDNEDLRSLIVRRLHRAGYEAFGVSL
                     GCEAVEKAFERPGTALLIDHNLPDMSGQELVALLKERGAAPPFIIMTGQGDERLAVQM
                     MKLGAFDYLVKNSELLDKLPETLGRLFGELETERRILEDRSILLDNIRTQVWYLTDDH
                     TYGAVNKAHA"
     assembly_gap    1432..1531
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1532..2767
                     /locus_tag="PK442_02945"
     CDS             <1532..2767
                     /locus_tag="PK442_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86636.1"
                     /translation="LRAAKENAEWLGQQAEAASKAKSAFLANTSHELRTPLNGAIGFL
                     ELLADTPLNELQKEYVGYIRTSAYTLLEVISEVLDISKIESDRFDLEYAQSDVFEVMR
                     KAVDAVRGTVLRKELRLELRIEEGTPRYAVVDPLRLKQVLVNLLGNAVKFTEKGFVEL
                     ALQCVALDAGRCAYTFSVRDTGIGIAPEVHRKLFEPFFQADASNTRKYGGVGLGITIC
                     EALLQKMESRLQLESAPGQGSRFFFTLCARCDAEPEETTNGERTNLLPAKGVPLPLRT
                     NRQAVVLLAEDQRLNRRLLRILVSKLVPGARIIEASDGEEAVRFFRSEAPDIVFMDLQ
                     MPLKDGCEAVAEIRSLERASSGGTAKAGIPIIAVTADVQKETRTFCLENGMDDFITKP
                     VDAWSLRCVLERCLESVLL"
     gene            3100..>4380
                     /locus_tag="PK442_02950"
     CDS             3100..>4380
                     /locus_tag="PK442_02950"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HOO86637.1"
                     /translation="MPLRRLFLLLMMLLLALVLVLTSFTSQRSTLKAFDDVERRFFIQ
                     DMARVQNRLDEELRVLRNYAADWGAWDSMYAFVEKGCKHRFDEMFDGQAMLAAGMEAL
                     LIFDTGRRLLFSIFEGECAAERKLPDHVSKGLKDAVPSLMERSSDGAFAEILLLGDAA
                     YLFGVSPVLRTNRTGPSQGVLLIGRSLGKLLPEMRKSLGTKFDLLPGRAADVPDRSAG
                     DVRIVSGEGEGAAVWQGRGNEFSVRIDVPRLIRQRGLEAVESSRLWILLTGAGTLGLV
                     LLLLDRVILRRLRKLREVSERIVAGESLTLRVDEEGPEEIFALSRSFNMLLQTLHDLV
                     MKIPDPFILADADGAITLANDAAHTVLSYGTDLKGTPLSSVLVEKEDDPGAGKKVAEK
                     DPDTKNGQSVFEASLVRADGSLLPVEVHRRSFTFG"
BASE COUNT          906 a         1212 c         1344 g          818 t
ORIGIN      
        1 ggcggaattg agctcctctc tgcaatttca ggtcagagag gccggggcgg cgcttcgcgt
       61 cggcaggttg ccctcgtgca gaggcgacgc cgtgcagctc actcaggttt tcgcgaacct
      121 tctgggcaac gcgctgaagt atcttgatcc ccaacgtccg ggggaaatag tcgtcgaggg
      181 gcggatcgag caggaccgcg ccgtctacct ggtgcgggac aacggtatcg ggatcgcgcc
      241 gcagcaccag gagaagattt tccagctctt ccacaggttg aatccatcgg cgaacgaagg
      301 cgaggggctt ggcctgacga tcgtcaggca gatccttgga agactggacg gggaggtcgt
      361 cgtggaatcg acgcccggaa ctggaagcac gttcacgatc acgctgcccg cagcgtcgaa
      421 aaagggagaa tgattatgga ggaggaagaa cgcatgcaca atgaagtcat catactcata
      481 gccgaggacg acgaggggca cgccgggctg atacggaaaa atttggcgcg cgcgggaatc
      541 tccaaccatg tggaacactt caaggacggg caggagatca tcgattttct ctttcgcatc
      601 gggaacgggc cgcacaggcg gtcgcacacc ccgtacattc tgctgctcga catccggatg
      661 cccaagctgg acggcacgga agtgctcaag cgcatcaaag aggaccacga actccgtaag
      721 atgccggtga ttatgatcac cacgaccgac gatccccggg aagtggaacg ctgtcacgaa
      781 ctggggtgca gcagctacat cacgaaacct gtggaatacg acgccttcgt caacgccgtt
      841 cgccagctgg ggctctacct tgcagtcgtg caggtgcctc aggtgaacgg cgtgcactga
      901 gagcggcgga gacccgaagg atggatggac gcgccgagca cggggacgcc ccggcacgaa
      961 acgttctcgt cgtagaggac aacgaagact tgcgaagcct gatcgtcaga aggcttcaca
     1021 gagcggggta cgaggcgttc ggcgtgtccc tcggatgcga ggctgtggag aaagccttcg
     1081 agcggcccgg aacggcgctc ctgatcgatc ataatttacc cgacatgtcc ggacaggagc
     1141 tggtcgcgct gctgaaagag cgcggtgcgg cgccgccttt tatcatcatg accggacagg
     1201 gagacgagcg actcgcagtg cagatgatga agctgggcgc cttcgattat ctggtgaaga
     1261 actccgaact gctggataaa ctgccggaga cgctggggcg cctcttcgga gagctggaaa
     1321 cggagcggcg aatcctcgaa gaccggagca tcctcctcga caacatccgg acgcaggtat
     1381 ggtacctcac cgacgaccat acctacggcg ccgtcaacaa ggcgcacgcg gnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctccgggcg gccaaggaga acgccgaatg
     1561 gctcgggcag caggccgaag ccgccagcaa agcgaagagc gcttttctgg cgaacaccag
     1621 ccatgaactc cgtacgcctc tcaacggtgc gatcggcttt cttgaactgc tcgcggacac
     1681 gcccctcaac gaactgcaga aggagtacgt cggctacata cggacgtccg cctacacgct
     1741 cctcgaagtc atcagcgaag tgctggacat ctcgaaaatc gaatccgacc ggtttgacct
     1801 tgagtacgcc cagagcgatg ttttcgaggt tatgcggaag gctgtggacg ccgtccgcgg
     1861 gactgtcttg cggaaggaac ttcgccttga actccgcatc gaagaaggga cgccgcggta
     1921 cgcagtcgtc gacccgctcc gtctgaaaca ggtgctggtg aaccttttgg gcaacgccgt
     1981 caagtttact gaaaaaggat tcgtcgagct tgcgcttcag tgcgtcgcgc ttgacgcagg
     2041 acgatgcgcc tatacgttct ccgtcaggga caccggcata ggtattgctc cggaggtcca
     2101 tcgcaaactc ttcgagccgt ttttccaggc cgacgcttcc aacaccagaa agtacggggg
     2161 agtcgggctc ggcatcacta tctgcgaagc gctgctgcag aagatggaga gccgcctcca
     2221 actggaaagc gcacccggcc agggaagccg cttcttcttc accctctgcg cacgatgcga
     2281 cgccgaaccg gaagaaacaa cgaacggaga gcggacgaac ctgctcccgg caaaaggggt
     2341 tcctcttccg ctgcgtacca accggcaagc cgtcgttctc ctcgccgagg atcagcggct
     2401 caaccgaagg ctgctgcgaa tcctcgtctc caagctcgtc ccgggggcgc gaatcatcga
     2461 ggcgtccgac ggagaagaag cggtccgctt cttcagaagc gaagcgcccg acatcgtctt
     2521 tatggatctg cagatgccgc tgaaggacgg gtgcgaagcg gtagcggaaa tacgttcgct
     2581 tgaacgggcc tcctccgggg gtacggcgaa ggccggaatt ccgattattg cagtcaccgc
     2641 cgatgtccag aaggaaacgc gaacgttctg tctcgagaac gggatggatg atttcattac
     2701 caaacctgtg gacgcgtgga gtctcagatg cgttctggag cgttgcctcg aaagtgtcct
     2761 cctctaagcg gagaaaagcg tacgtatctg ctgagagtat ccgtgcgttc cgggagcatc
     2821 gggacgcatt ttttttgcca aaataaagat caataaagat cagtgattac gtactctttt
     2881 ttcatgaacg ttctgggcgg atgggctgtt ttcctaggga gtatgcggcg aagttcgcgg
     2941 tttgtctcgt tgaaaaaaat tagtctttgg gatgagtaaa gtttttgttg cgaagctcgc
     3001 cattcaagga gataataagc aaagtgaaat acttcatatt ctttcaggct gctgtgaccg
     3061 cactctctca gcggaagaaa tttagcgagg agatcgccca tgcccttgcg ccgactgttc
     3121 ttgctcttga tgatgctttt gctggccctt gtactcgtgc tgacctcctt cacctcgcag
     3181 cgaagtacgc tcaaagcgtt cgacgacgtg gagcgccgtt tcttcattca ggacatggcg
     3241 agggttcaga accgcctgga cgaggaactc cgggtgctgc gcaactatgc ggcggactgg
     3301 ggcgcatggg acagcatgta cgccttcgtg gaaaagggct gcaaacaccg gttcgacgag
     3361 atgttcgacg gacaggcgat gcttgccgcc ggcatggagg cgctcttgat tttcgacacc
     3421 gggcggcgtc tcctgttctc catcttcgag ggggagtgcg cagcggagcg gaagctcccc
     3481 gatcacgtgt ccaagggcct gaaggacgct gttccttcgc tgatggagcg ctcatctgac
     3541 ggagcgttcg ccgagattct gctcctgggc gacgcggcct acctgttcgg agtcagcccg
     3601 gtgctgagaa cgaacaggac ggggccgtcg caaggagtgc tcctcatcgg gaggtcgctg
     3661 gggaaacttc tgcccgagat gcggaagagc ctcggcacga agttcgacct ccttccgggg
     3721 cgcgctgcgg atgttccgga ccgctcggcg ggagatgtcc ggatcgtctc cggagaaggg
     3781 gagggcgcgg ccgtatggca ggggagaggg aacgaattct ccgtccgcat cgatgtcccc
     3841 aggctcatcc gccagcgggg gctggaagct gtcgaatcgt cgcgtctctg gattctgctc
     3901 accggcgcgg ggactctcgg cctggtgctg cttctcctcg acagggtgat tctgcgccgt
     3961 ctgcggaagc ttcgcgaggt ctccgagcga atcgtcgccg gagagtcgct cacgctgcgg
     4021 gtggacgaag aggggcccga agagatcttc gcgctctcgc gctcgttcaa catgctgttg
     4081 cagacgctgc acgatctcgt gatgaaaatc cccgatcctt tcatccttgc cgacgccgac
     4141 ggcgccatta cgctggccaa cgacgcggcg cacactgtcc tgagctacgg gaccgacctt
     4201 aaaggaacgc ctctctcctc cgttctcgtc gagaaggagg atgatcccgg cgccggaaag
     4261 aaagtcgcgg aaaaggatcc ggatacgaag aacggacaaa gcgtcttcga agcgtcgctc
     4321 gtcagggcgg acggttcgct gcttcccgtc gaggtgcacc gccggagctt cactttcggc
//
LOCUS       DAOSWW010000092         4373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_13679_length_4373_cov_3.225799, whole genome shotgun sequence.
ACCESSION   DAOSWW010000092 DAOSWW010000000
VERSION     DAOSWW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13679_length_4373_cov_3.225799"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK442_02955"
     CDS             complement(<1..568)
                     /locus_tag="PK442_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO86638.1"
                     /translation="MVRILLADDHPLTRSGLAAWIAKEEGMQLVGETEDGESAWSAIL
                     ELHPDLAMLDIEMPDCSGIDIASRVLKRGLATNVLILTAYSAHQYVMASVRAGAKGFI
                     LKSAPLAQLRKAIFQTAAGEFYLDPSVSLARTEGDVEQLSERELEVLVLAGQGLPGHE
                     VARLLSITERTVAAHLTSVYGKLGAKNKT"
     gene            complement(663..2084)
                     /locus_tag="PK442_02960"
     CDS             complement(663..2084)
                     /locus_tag="PK442_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO86639.1"
                     /translation="MKKHFLLLLMGTVTLFTAGNFFVSLHAFRTQESAMDSMMRSYVL
                     DLAENFSNTASFTGGRGQAQRHGTPGMMRFRMLSADPALQSPEAGGLLVISRERRIIT
                     ASPGAERLLPLWKGTAPEEEPREVLDAKGESYYVVVRPLDSGNVVLAAVSRTYLLAPL
                     TGVWKTWILSAMATSAVVLVGMFLLWYWLAVPLRGIAERLGNVGWGKERSIRLYNGPI
                     SELEALSSVIERLATESFEKEELKKRYVTDLVQAQESTRGQLARELHDGALQSTVAAV
                     KRIQLAKQSVPSDANSPLRAHLDTAEEVVQTAAGEIRSYCETLSPSWVRLGLSSALEE
                     IAGRLAQAYEGVETDVDIGVELEAAVAALPEDHVLALARIFQEAASNSVRHGGARFIQ
                     VELRKEGDALLFSVEDDGSGFDADAYAGTDFEELRTTGHRGLANINERVRLLRGTMRI
                     MSRPGESCRIDILFPPPSPGETR"
     gene            complement(2081..2731)
                     /locus_tag="PK442_02965"
     CDS             complement(2081..2731)
                     /locus_tag="PK442_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO86640.1"
                     /translation="MLRILLADDHPLTRSGLAAWLEAEEGVELVGQAADGESAWTMLL
                     ETQPDLALLDIEMPNGSGIEIAERIMKRKLPTNVLMLTAYSAQQYVMASVRAGAKGFI
                     LKSAPFDQLRQAIADTSRGIFYLDPSVSLVSDEAPAEELSEREKEVLILTAQGLPSSE
                     TARLLSITKRTVAAHLTSIYAKLGAKNKTEAILLALKRGVVLLDQLRFIDGKGESL"
     gene            2972..3496
                     /locus_tag="PK442_02970"
     CDS             2972..3496
                     /locus_tag="PK442_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86641.1"
                     /translation="MKKTTIFALVAVLVLVAASGAFAWGGRGGRGMVAPGTEGTTAPM
                     MQRGMAAPGAPGGTAVPMMGRGMAGRTANGRGAMMGNQQMMKGMRGRGASGGMGGRGM
                     MSVWANVEIPQEIRDKQVEMQKLSVDMRAEMQKKPVDRAKVEELYKKRVELRDALSGW
                     RMKTRLDTIEKLQK"
     gene            3690..>4373
                     /locus_tag="PK442_02975"
     CDS             3690..>4373
                     /locus_tag="PK442_02975"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOO86642.1"
                     /translation="MGTHDRGYKLLFSNKEMMRDLLLEFVTEEWVDGLDLDTLDRVHD
                     SFVTDDLRERFDDIIWRVSFRGRMVYLCLIVEFQSSPDRWIAGRLMAYIGLLYGDIVK
                     RNEIVDGFFPPVLPIVLYSGEGRWTVPLSLEEMLHPASSVLGRYAPHLRYLLLDESAY
                     SEERLAQSKNLAAFLFRMENSRTPDHLLEIVRSLGGVLDGEEHRHLRRSFAVFVRNAL
                     LPKKTRRRNF"
BASE COUNT          778 a         1298 c         1479 g          818 t
ORIGIN      
        1 cggtcttgtt cttcgcgccg agcttgccgt agaccgatgt gaggtgtgcc gcgacggtcc
       61 gttcggtgat ggagagcaga cgtgcgacct cgtggccagg gagcccctgt ccggcgagga
      121 cgagcacctc cagttcgcgt tcggagagct gttcgacgtc gccctccgtc cgggccagcg
      181 agaccgaggg gtcgaggtag aactcgcccg ccgccgtctg gaagatcgcc ttgcggagct
      241 gcgccagcgg ggcggacttg aggatgaacc cttttgcgcc ggccctgacc gaggccatca
      301 cgtactggtg cgcgctgtag gccgtgagga tgaggacgtt cgtggccagt ccgcgcttga
      361 gcacacgcga ggcgatgtcg atgccgctgc agtcgggcat ctcgatgtcg agcatcgcca
      421 ggtcggggtg cagttcaaga atagccgacc atgcggactc tccgtcctcg gtttccccga
      481 cgagctgcat tccctcctcc ttcgctatcc acgcggcgag gccggagcgg gtcagagggt
      541 gatcgtcggc gagcagaata cgtaccatgg caaagcccct ttctgaacgg gattgtgtgt
      601 gcggccgccg catttcgacg ccacggaggc tcgacgctgt tgctggaacg ccgtttctcc
      661 tgtcaacgcg tttctcccgg cgagggggga gggaagagga tatcgatgcg gcagctctct
      721 cccgggcgtg acatgatccg catcgttccg cggagcaggc ggacgcgctc gttgatgttc
      781 gccagaccgc gatgccccgt cgtccgcagc tcttcaaagt cggttccggc gtacgcgtcg
      841 gcgtcgaagc ccgagccgtc gtcctccacg gagaagagga gcgcgtcccc ctccttgcga
      901 agctcgacct gtatgaagcg cgccccgccg tgccggacgg agttcgacgc ggcctcctga
      961 aagatgcgcg ccagtgcgag cacgtggtcc tcggggagtg cggcgaccgc cgcttcaagc
     1021 tccacgccga tgtccacgtc ggtctccacc ccttcgtacg cctgcgcgag acgtccggcg
     1081 atctcctcga gcgcgctgga gagccccagc cttacccagg agggggagag cgtctcgcag
     1141 tagctccgga tctctcccgc cgccgtctgc acaacctcct cggcggtgtc gaggtgcgcg
     1201 cggagggggg agttcgcatc cgaaggcacg gactgtttcg caagctggat gcgcttgacc
     1261 gccgccacgg tcgattgcag cgcgccgtcg tgcagctccc gggcaagctg tccccgggtg
     1321 ctctcctgag cctgcacaag gtcggtgacg tagcgttttt tcagctcctc cttctcgaac
     1381 gactccgttg cgagccgttc gatgacggag gagagcgcct ccagctcgga gatcggcccg
     1441 ttgtagagac ggatggagcg ttccttcccc cacccgacgt tccccaggcg ttccgcgatt
     1501 ccccgcagcg gcacggcgag ccagtaccag aggaggaaca tgccgacgag aacgactgcc
     1561 gaagttgcca tcgcggagag aatccaggtc ttccagacgc cggtcagcgg ggcgaggagg
     1621 tacgtccggg agactgccgc gaggacgacg ttcccgctgt cgagcggtcg cacgacgacg
     1681 tagtagctct cgcccttcgc gtcgaggacc tcgcggggtt cctcttcggg tgcggttcct
     1741 ttccagaggg ggagcagacg ttccgcaccg ggcgacgcgg tgatgatccg cctttcgcgc
     1801 gagatcacga ggagccctcc cgcctcgggg ctctgcagcg cggggtcggc ggagagcatc
     1861 cggaaccgca tcatccccgg agttccgtgg cgctgcgctt ggcctctgcc tccggtgaac
     1921 gaggcggtgt tggagaagtt ctcggcgagg tcgaggacgt acgagcgcat catcgaatcc
     1981 atcgcggact cctgcgtcct gaaggcgtgg agggagacga agaagttccc ggcggtgaag
     2041 agcgtcaccg tccccatcag cagcagaagg aagtgcttct tcatagggat tcgcccttgc
     2101 cgtcgatgaa ccggagctgg tccagcagga cgacgcccct cttgagggcg agcaggatgg
     2161 cctcggtctt gttcttcgcc cccagcttcg cgtagatgga ggtcaggtgt gccgcgaccg
     2221 ttcgcttggt gatggagagc aggcgcgccg tctcggagga gggaagcccc tgcgccgtga
     2281 ggatgagcac ctccttctcg cgctcggaga gctcctccgc gggagcctcg tccgagacca
     2341 gcgagacgga ggggtcgagg tagaagatgc cgcgcgacgt gtccgcgatg gcctgacgca
     2401 gctggtcgaa cggagcggac ttcagaatga accccttggc cccggcgcgc accgacgcca
     2461 tcacgtactg ctgggcgctg taggcggtga gcatgaggac gttggtcggc agcttccgct
     2521 tcatgatgcg ctcggcgatc tcaatgccgc tgccgttggg catctcaatg tcgagcagcg
     2581 caaggtcggg ctgcgtttca agcagcatcg tccacgcgga ctctccgtcc gcggcctgtc
     2641 cgacgagctc gacgccctcc tcggcctcca gccaggccgc gaggccggag cgtgtgagcg
     2701 gatggtcgtc ggcgagcagt attcgcagca tctcggcgtc cccctttcaa aagccgcgtt
     2761 ccgcggtatg cggcgcggcg tgaagattct ctccctatag tatatccgtt ttttgaaccg
     2821 aacgaagaaa gaatacgttc ttcgggtttc ttcggaaatt gtaacttgtt acagtcggga
     2881 gagcggtagt tcggaccatg cgcgtccggc cgcgccgcgg tatcctgtac ccgtcacatc
     2941 aaaacacact tttgaaaggc ggggttcact catgaaaaag acaacgatat tcgcgttggt
     3001 ggcggttctg gtactggtag cggcaagcgg agcgttcgcg tggggcggac ggggaggcag
     3061 aggaatggtt gctcccggaa ccgaaggaac gacggcgccc atgatgcaga gaggtatggc
     3121 cgctcccggc gcacccggcg gcacggcggt tccgatgatg ggaagaggca tggccggacg
     3181 tactgcgaac ggacggggcg ccatgatggg caatcagcag atgatgaaag ggatgcgcgg
     3241 tcgcggagcg agcggcggca tgggcggacg cggcatgatg agcgtgtggg cgaacgtcga
     3301 gattccgcag gagattcgcg acaagcaggt cgagatgcag aaactctcag ttgatatgcg
     3361 cgccgaaatg cagaagaagc cggtcgaccg cgccaaggtc gaggagctgt acaagaagcg
     3421 cgtggagctg cgcgacgccc tttccggctg gcggatgaag acaaggctcg acacgatcga
     3481 aaagctccag aagtaagcga aaaaacggcg cgcctcccga aagcagcgct gcaacgaaga
     3541 gagggcgagg actccggtcc ccgccctctc ttgtgcgaac gtatccggca cggccaaggt
     3601 gcgcaagcgt gcgagcggtc tcttgaagcg gttaagaagc atggcgccct tcgcttgcgg
     3661 attcctgtcg ccgattctat aatgttcgta tgggaactca cgacagagga tacaagcttc
     3721 tcttttcgaa taaagagatg atgcgcgacc ttctccttga attcgtcacg gaggagtggg
     3781 tcgacggtct ggatttggat acgctggaca gggtgcacga ttccttcgtg accgacgatt
     3841 tacgggagcg gttcgacgac attatctgga gggtgagctt cagagggaga atggtctatc
     3901 tctgtctgat cgtcgagttc cagtcctcgc cggaccggtg gatcgccgga cggcttatgg
     3961 cgtatatcgg tttgctctac ggcgatatcg tcaaacggaa cgagatcgtc gacggttttt
     4021 tccctccggt gctgccgatc gtgctctatt cgggagaggg gagatggacc gtccccctct
     4081 ctttggagga gatgctgcac ccggcttcgt cggttctcgg gcgctatgct ccgcatttgc
     4141 gctatctgct tctcgacgag agcgcataca gcgaggagcg gctcgcgcag tcgaagaatc
     4201 tggcggcgtt tctttttcgc atggagaaca gcaggacgcc cgatcacctg ctggagatag
     4261 tgcggtcgct cggcggcgtg ctggatggag aggaacacag gcatctgagg cgctcgttcg
     4321 cggttttcgt gcggaacgcg ctgctgccga agaagacccg cagaaggaat ttt
//
LOCUS       DAOSWW010000093         4335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_13844_length_4335_cov_4.145561, whole genome shotgun sequence.
ACCESSION   DAOSWW010000093 DAOSWW010000000
VERSION     DAOSWW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13844_length_4335_cov_4.145561"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK442_02980"
     CDS             complement(<1..387)
                     /locus_tag="PK442_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86643.1"
                     /translation="MDRSREVVFLEREMEDLTVYIDGDRQVADILARVPTRSGRDVWL
                     LLHAEIQGPGGGDLPTRMFCYNSTLRVTHLKEKSDIADVVSCAVLTAKRPSAEEDRYL
                     RESYGNRMLYEYPMLKLWELDADALEE"
     gene            complement(683..946)
                     /locus_tag="PK442_02985"
     CDS             complement(683..946)
                     /locus_tag="PK442_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86644.1"
                     /translation="MSYFGQQCHFQNLGVRKQELGPILSFPEGLREASFFFRKIHVFG
                     QKRLNTPEGIFIGREETKREAALRMLRRGDERLPGGGGRSSPS"
     gene            complement(1055..1978)
                     /locus_tag="PK442_02990"
     CDS             complement(1055..1978)
                     /locus_tag="PK442_02990"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOO86645.1"
                     /translation="MFINAHRPAALFEEFFWTLNNHFRSTTGLGHRKAKFRFKNRLFS
                     FDSTTISLCLSLFPWANYRRTKGGVKVHVLLDHDDYMPSFISITDAKRHDNNESKQLD
                     LKPGSIVAVDRGYIDYEQFRRWNDKGVFFVTRMKSNASYDVVESREVPQKRNIVFDEI
                     IVLTGETTSEKFPGPLRRIVVHDKKKDEDIVLLTNHLNFGSTTISSIYKDRWEIELFF
                     KTLKQNLKVKTFIGTSENALRIQIWTAMIALLVLKWLHYLSKSGWSFSNLASMLRLSL
                     FTYRELRVWLDKPCDTPPLTPDLAQLRPNLS"
     gene            complement(1980..2294)
                     /locus_tag="PK442_02995"
     CDS             complement(1980..2294)
                     /locus_tag="PK442_02995"
                     /inference="COORDINATES: protein motif:HMM:NF025652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HOO86646.1"
                     /translation="MIKAASLFSQILSTIRTAANFDNLVAKHEAEKNAKGFESKTQLV
                     SMLFCHLAKADSLREIVNGLACCNGMMSHIGLKKAHPPLLSLLCHKQATDEAVSRVES
                     FA"
     gene            complement(2773..2858)
                     /locus_tag="PK442_03000"
     tRNA            complement(2773..2858)
                     /locus_tag="PK442_03000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2823..2825),aa:Leu,seq:caa)
     gene            complement(2999..3328)
                     /locus_tag="PK442_03005"
     CDS             complement(2999..3328)
                     /locus_tag="PK442_03005"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOO86647.1"
                     /translation="MTYEDFCQVAEKMIENLPPKLFEQLNGGVNVVRQKKEDGEYVLM
                     GEYVEDPIMGNTVYLYYGTFAEELKGAKRSEWIEEIEETLLHELRHHVESLAGVDYLS
                     FEEMQDL"
     gene            3466..3936
                     /locus_tag="PK442_03010"
     CDS             3466..3936
                     /locus_tag="PK442_03010"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HOO86648.1"
                     /translation="MNRLIIRHFIPAADSGVRCVPCEETSGYLQSMMESLAPKLVNLD
                     IQLVLQSLEIHEITEANCGKLNYISFFAPELGLEVERPIEEVLNAGVTFERCENCSLA
                     DGTPFAMRTLKTEGSSFAALPPGILSDALIRVVFSTMGSCGKGSCASCAGCGEH"
     gene            3956..>4335
                     /locus_tag="PK442_03015"
     CDS             3956..>4335
                     /locus_tag="PK442_03015"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOO86649.1"
                     /translation="MSEVWITTKGGDKGETSIGNGERVPKDHARVETYGTLDECQAHI
                     GMARSLCEYPAIRDYLCLLEKEMSLLMGYIALYPGLTCPEITRLEEITHVVGEVTGKT
                     FRFVRPGDSTPGAALHIARTVARRA"
BASE COUNT          930 a         1189 c         1131 g         1085 t
ORIGIN      
        1 ctcctcgagt gcgtcggcgt ccagctccca cagcttcagc atcgggtatt cgtagagcat
       61 cctgtttccg tagctctccc gcagatagcg gtcctcctcc gcggacgggc gcttcgccgt
      121 caggacggcg caggagacca cgtcggcgat gtcgctcttt tccttcaggt gcgtcacacg
      181 aagagtggag ttgtagcaga acatccgcgt cggaaggtcc ccgcctccgg ggccctgtat
      241 ctcggcgtga aggagaagcc atacgtcgcg ccccgatctg gtcggcacac gggccaggat
      301 gtccgcgacc tgcctgtctc cgtcgatgta gacggtcaga tcctccatct ccctctccag
      361 aaagaccacc tcgcgcgatc ggtccatgtc ctcggccagc gcggtcatga ttctgcacaa
      421 tacgaggtag aagaagtgct tgatgaccgc gcgccagtgt tcgtcgattc tcctccgctc
      481 cctcttctcc gcgtcctccg tagccgcgct cgcggcgctt tccgtcgcgg cggggcccta
      541 ggacccgaga ctgggattcg ttcgttccat atcggcggcc attttgctcg ccctccttcg
      601 gcttttctcc gcatcgcaag gggtggcgga gcttccttcc gcttctcttc acggtacagt
      661 atagcccgct ctcttttctt cttcaagagg gagaagaacg acctccgccg ccagggagac
      721 gctcatcccc cctgcgcagc atcctgagag cggcctcgcg cttggtctcc tccctgccga
      781 tgaagatacc ctcgggcgta ttcaaccttt tctgtccgaa tacatgaatc ttacggaaga
      841 agaaagacgc ttcgcggaga ccttccggaa aggagagtat cggcccgagc tcctgtttgc
      901 ggacgccgag attctggaaa tgacactgct gtccaaaata actcacgcta agaaattgtt
      961 tccgctgttt gcaggggtct gaatcgcgat tttgaacaaa gttgaaacca tgcccttttt
     1021 ctctgcacct cgaaaattaa accggctgtc caaatcacga gaggttcggt ctcagctgcg
     1081 ccaagtcagg agtcagcggc ggcgtatcgc acggtttgtc cagccagacg cgcaactccc
     1141 gataagtgaa caggctaagc cgaagcatcg acgccagatt cgaaaaggac catccggatt
     1201 tcgacagata gtggagccac ttcaaaacaa gcaaagcgat catggctgtc caaatctgga
     1261 tgcgaagagc gttctcgctc gttcctatga acgtcttcac cttcaggttc tgcttcaagg
     1321 tcttgaagaa cagttcgatc tcccatcggt ccttgtagat ggacgaaatg gtggtcgagc
     1381 cgaagttcag gtggttcgtc agcagcacga tatcctcgtc cttcttcttg tcgtgcacca
     1441 cgattctccg cagcggtccg gggaactttt ccgacgtcgt ttctccggtc aacacgatga
     1501 tctcgtcgaa gacgatgttc ctcttctgcg gaacttccct gctttcaacg acgtcgtaac
     1561 tcgcgttgct cttcattctg gtgacgaaga acacgccctt gtcgttccat ctccggaact
     1621 gttcgtagtc gatgtacccc ctgtccaccg ccacgatgga gccgggcttg aggtcgagct
     1681 gtttcgactc gttgttgtcg tgccgtttcg cgtccgtgat cgagatgaaa gacggcatgt
     1741 agtcgtcgtg gtcgagcagc acgtgtacct tcactccgcc ctttgttctc ctgtagttcg
     1801 cccacggaaa gagcgacaga cagaggctga tggtggtcga atcgaaggag aagagcctgt
     1861 tcttgaagcg gaacttcgcc tttctgtgcc ccagtcccgt ggtcgagcgg aagtgattgt
     1921 tcagagtcca gaagaactcc tcgaagagag cggcgggacg atgcgcgttg atgaacaatc
     1981 taagcgaacg actcgactcg ggaaaccgcc tcgtcggtcg cctgcttatg gcataggaga
     2041 gagaggagcg gcgggtgcgc cttcttcagc cctatgtggg acatcattcc gttgcagcag
     2101 gccaggccgt taactatctc tcgaagcgaa tcggcctttg cgagatgaca gaagagcatg
     2161 gatacgagct gcgtcttcga ttcgaagccc ttggcgtttt tctccgcctc gtgtttggcc
     2221 accaggttgt caaagttcgc tgcggtgcgg atggtcgaaa gtatttgact gaacagactc
     2281 gctgccttta tcatttggtt tgcccccttt ttttgttttg agatcttttt gtgttggcaa
     2341 accaatgata tctcaaagcc ggggggcttt ttaatttttc agaaacctat tttggacaag
     2401 agtgccgtaa gatatgaaag aacggcttgc cgaagaagtg gtccggactg gaaagagtcc
     2461 ggataccttt tgaaaaaacg cctgatgtgg ctctcgtaat cctgaatggt acgctcgctt
     2521 cttccctccg ctcccttgaa aatgagaaaa tagcgcaaag cctcttccca tctggtttcg
     2581 gagctgtttt ggtcttcgtt tttccctaac atttcggggc ctccttttcg ccgttcggac
     2641 gatgaaagaa gaccccgaaa atccgccgca atcccctcgt tttacatcac tacggattcg
     2701 tcatttttga ggggataaaa aacaatgctt ttacacttta aatcggctct ttaagccagt
     2761 catttcaatg attggtgccg ggggggggac tcgaaccccc acgtggtcgc ccacggcgga
     2821 ttttgagtcc gctgcgtcta ccattccgcc accccggcat gcactgcggt atcttaatcg
     2881 aaaaaaacgg acatgtcaac ggagttaaga acaattttgc gcggcgacaa caagaatcga
     2941 agtgtttttc ctcattttaa gatagcgttt ccctccagtg ttttttaggg ggaagtcgct
     3001 acagatcctg catctcctcg aaagagagat agtctacacc ggccagagat tccacgtggt
     3061 ggcgcagttc atggagaagc gtctcttcga tttcctcgat ccattcgctg cgtttcgcac
     3121 cttttaattc ttctgcaaat gtgccatagt aaaggtagac ggtattcccc ataatagggt
     3181 cttcaacgta ctcgcccata agaacatact ctccgtcctc ttttttctgc cgaacgacat
     3241 tcacgcctcc gttgagctgc tcgaagagct tcgggggcag gttttcaatc attttttcag
     3301 ctacctggca aaaatcttcg tacgtcactg cgcacctctc cttgttcttt tcttcatcac
     3361 gctctccttg ttttcagaga ggaatcgtgt ccataccttt tcccttaaaa caccctccga
     3421 gtgtataatg taatgcatgg atcacatcaa ggaggtcgat cgagaatgaa cagactgatt
     3481 atacgacatt tcatacccgc tgcggattcc ggtgttcgct gcgtcccctg cgaagaaaca
     3541 tccggttatc ttcagtcgat gatggagagt ctcgctccga aactcgtcaa tctggatatc
     3601 cagcttgtgc ttcagagcct ggagatccat gagatcaccg aagcaaattg cggcaaactg
     3661 aactatattt ccttcttcgc accggaattg gggcttgaag tggagcgccc gatcgaggag
     3721 gttctgaacg caggagttac ttttgaacgc tgtgaaaact gttctctagc agacgggact
     3781 ccctttgcaa tgaggacgct caagactgag ggcagttcgt tcgcggcgct tcctcccggc
     3841 atactcagcg atgctctgat acgcgtggtc ttttccacaa tgggcagctg cggaaaaggg
     3901 agctgcgctt catgcgccgg atgcggagag cattgagaaa gtctggttta tcccgatgag
     3961 cgaagtctgg atcacgacaa agggcggcga taaaggagag accagcattg gtaacggcga
     4021 gagagtgccc aaagaccatg cccgggtaga aacctacggg actttggatg aatgccaggc
     4081 tcatatagga atggcgcgtt ccctgtgtga atacccggca attcgtgact acctctgcct
     4141 tctcgaaaaa gagatgagcc ttctaatggg gtatatagcc ctgtatccgg ggctgacgtg
     4201 ccctgaaatt accaggcttg aggagattac gcatgttgtc ggcgaagtga caggaaaaac
     4261 ttttcgtttt gtccggccgg gtgactcaac tcctggagcc gcactccata ttgccagaac
     4321 tgtcgccagg agagc
//
LOCUS       DAOSWW010000094         4334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_13847_length_4334_cov_3.084132, whole genome shotgun sequence.
ACCESSION   DAOSWW010000094 DAOSWW010000000
VERSION     DAOSWW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13847_length_4334_cov_3.084132"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /gene="phoU"
                     /locus_tag="PK442_03020"
     CDS             complement(<1..652)
                     /gene="phoU"
                     /locus_tag="PK442_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOO86650.1"
                     /translation="MTKNATIQSFLYATDRERIVSKVVLMGERAAQLLENSVSALLDQ
                     DSALARRIIAEDDAIDQMEGEIDQECLGSIAMRSPGKDELYFVFAVLKIITDLERIGD
                     QAVNIAEKALKVTPAVVFPRRDALLKMLRVVLSMFRQALQAFRNEDKHMAATLWKRDD
                     EVDSMYAQCYADFLHDAVAHSGNDDPQTMEVGALELWTARHLERAGDHVINIVERVY"
     gene            complement(678..2267)
                     /locus_tag="PK442_03025"
     CDS             complement(678..2267)
                     /locus_tag="PK442_03025"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOO86651.1"
                     /translation="MLSPFFAAINLFGGLALFLYGVEETTTAFGSSFGGKSKELLIRF
                     TRRKPMAFLFGAALSAIAQGSTVATSFAIGFVDVGMLSFAGSVVVMMGASVGGTFVTF
                     LLSLDIAAFSPLLFGASFLMVRFGKRTVYRIGCVLQGLSLVLLGMFVLKLGVGPLLQD
                     PRFSDMATVAGSNSLLVGIAAFVATAVLQSSSAVMALAVTLAVSGALPATAILPVVCG
                     AHVGSSVTVLLAGFSGRQNARKLGVCTFVYKFLGVLFILPFFPWIQGFMASGGGLAAK
                     VVQAQVGVTLFNAVVFYPFTDILAHFSARIAEQAGGERLGAPVYLDDELTDVPSLAIL
                     LLSKEMIRLANYIEMYCQVLFAPEKSLEGFEELPFAIRELSEACQEYMFEIHIPSDDD
                     ALRENYSTVSYSMVSFRQMAKILSGELRLLAEAGVAAQFVRELGEKTWRELSRLALTD
                     IRLALRAFALADADFASEAGRYEREFEELDRRIRLRLGVDALNRRELAPLLDFMSQIF
                     LLVKTSLEVARGEARRDREAG"
     gene            complement(2267..3757)
                     /locus_tag="PK442_03030"
     CDS             complement(2267..3757)
                     /locus_tag="PK442_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO86652.1"
                     /translation="MDLFSHLLDGLGALMGITPLLVIMAGAAIGILAGAMPGISPSMA
                     VALLLPFSFGMTPSMGLVLLCAIYLASNYGGSITAVMVNTPGTPSAVVTAFDGYPLTR
                     KGDAGTALGVSLVASVAGGFIGIIILVLFSAPLARMALKFWPAEYFALAILGLSTIAS
                     LGGGRWLESFMAVLLGLLLNTIGLDPLSGVSRFTFDVVRLYDGFSFIPVLIGLFALSE
                     VFSGIESGEVGIPPVQPDKERSPWPTVREYLKLKYSMIRAGVLGTVIGVFPGAGGTIA
                     SFIAYDVEKRLSKEPESFGKGAPAGVAAAEASNSASVGGALVPLLTLGIPGSSSAAVL
                     IGALMIHDLQPGPELFTRHPEVVYTLFSSLFVANIFLLFLGLLGARLWIKAALIPKRL
                     LYPMIFAFSFIGSYAVRSSLFDVGVCLAFGLLGWMLSRSKIPVSPIVLGMILGTMIEK
                     NLRTTIMMGGVSLFFQRPLSLTLLLLAFASVVIPYIRERKRAGGGS"
     gene            complement(3770..>4334)
                     /locus_tag="PK442_03035"
     CDS             complement(3770..>4334)
                     /locus_tag="PK442_03035"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOO86653.1"
                     /translation="GGSMETLLLFLSGADSFPVWILSFLAVIALTAFLTRGKHAPFRG
                     RIIICVSLLFLAFAFFLLSFGLEEIEAVGSSARTAPRLWAAGLAFFTLDQLRRTLLGK
                     APEDPKTGDVQRVLLAAAIVAFSLWGMEIVGYLISSGGMILLLLLLFGERRLFILLSL
                     TGGWMLFSWFVFIRLLHLSLPAGIYFG"
BASE COUNT         1125 a         1272 c         1212 g          725 t
ORIGIN      
        1 aatagacccg ctccacgata ttgatgacat gatctcccgc tcgttccaga tgacgggcgg
       61 tccacagctc cagcgcgcct acttccatcg tctgcggatc gtcgtttccc gaatgggcga
      121 ctgcgtcatg taaaaagtcc gcataacact gagcatacat ggaatccacc tcgtcgtcgc
      181 gcttccacag cgtggcggcc atgtgcttgt cctcgttcct gaaggcctgc agcgcctggc
      241 ggaacatcga aagaaccacc cgcagcatct tcaaaagagc gtcccgccgg gggaagacga
      301 ctgccggcgt tactttcagc gccttctccg cgatgttcac cgcctggtcg ccgattcgtt
      361 ccaggtcggt aatgatcttg agaactgcga agacgaagta cagttcatcc ttaccgggcg
      421 agcgcatagc gatggatccg agacactcct ggtcgatctc cccttccatc tggtcgatcg
      481 cgtcatcctc ggctataatc cgccgtgcga gcgccgaatc ctggtcaaga agcgcgctca
      541 ccgagttttc aagaagctgg gcagcccgct cccccatcaa cacgaccttg gatactatcc
      601 gctctctgtc tgttgcgtac aaaaaagact gtatcgttgc atttttcgtc accgggcgac
      661 ttcacctccc cgagatatca gcccgcttcg cggtcgcgac gggcttctcc cctggcaact
      721 tcaagagacg tcttcacaag cagaaagatc tgcgacatga aatcgagcag aggagccagc
      781 tctcgtctat tgagggcgtc gactccaagt ctgagacgta tccgcctgtc cagctcttcg
      841 aattctcttt catagcgtcc ggcttccgaa gcaaaatcgg cgtccgcaag ggcaaaagcc
      901 ctgagcgcaa gccggatatc cgtcagcgca agcctggaga gttcccgcca ggttttctcc
      961 cccagctcgc ggacaaactg cgccgccact ccagcctcgg caagaagacg cagttcgccg
     1021 gagaggattt tggccatctg acgaaaggat accatagaat aagagacagt ggagtagttt
     1081 tcacgaagcg catcgtcatc ggacggaata tgaatctcga acatgtactc ctggcaagct
     1141 tccgaaagtt cgcgtatggc gaagggaagt tcctcgaacc cttcaaggct tttctccggc
     1201 gcaaagagta cctgacagta catttcaatg tagttcgcga gacgaatcat ctcttttgaa
     1261 agcagcagaa tcgcaagcga ggggacgtcc gtcaattcgt cgtcaagata gaccggcgcc
     1321 ccaagacgtt cccctcccgc ctgctcggcg attcgtgcgg aaaaatgcgc gaggatgtcc
     1381 gtaaacggat aaaagacaac cgcattgaaa agggtaacgc ccacctgcgc ttggaccacc
     1441 ttggcggcaa gcccccctcc tgaagccata aaaccctgaa tccacgggaa gaacgggaga
     1501 atgaagagga cgcctaaaaa cttatagacg aacgtacaca cgccgagctt ccgcgcgttc
     1561 tgcctcccgg aaaagcctgc gagcagcacc gtgacggacg aaccgacgtg ggctccgcag
     1621 actaccggaa gaatcgcggt agcgggaagg gccccggaga ctgcgagggt aacggcaagg
     1681 gccatcaccg ccgagctgct ctggagaacg gccgtcgcca cgaaagctgc tattccgaca
     1741 agaaggctgt tgcttcccgc aacggttgcc atatccgaaa accggggatc ttgcaggaga
     1801 ggtcctaccc ccagcttcag gacgaacatg ccgagaagaa cgagcgacaa tccctggaga
     1861 acgcagccta tccgatagac agttctcttc ccgaatcgca ccatcaggaa actcgccccg
     1921 aaaagcagcg gggagaatgc cgcgatatca aggctgagca ggaaggtaac aaacgtacct
     1981 cccacgctcg cgcccatcat tacgacgacc gaaccggcga acgagagcat gcccacgtcg
     2041 acgaaaccga tggcgaaaga tgtggcgacg gtgctcccct gtgcaattgc agagagcgcc
     2101 gcgccgaaga gaaacgccat cggcttcctt cgtgtaaatc ggatcagcag ctctttcgac
     2161 tttccgccga agctggagcc gaacgctgtg gtcgtctcct ccactccgta gagaaagagc
     2221 gccaggccgc cgaaaagatt aatcgctgca aagaacggag agagcactac gatcccccgc
     2281 cggccctttt tctctccctg atgtaaggaa tcaccacgga ggcgaaagcc agcagaagca
     2341 gcgtcagcga aagaggccgc tggaagaaga gagagacgcc gcccatcatg atggtcgttc
     2401 gaaggttctt ttcaatcatc gtcccgagta tcatacccag gacgataggc gagacgggta
     2461 tcttgcttct ggaaagcatc cacccgagaa gaccgaacgc gaggcagacg ccgacgtcga
     2521 agagcgagga gcgcacggca tagcttccga tgaatgaaaa agcaaaaatc atagggtaga
     2581 gcagacgttt cgggatgagc gcagccttta tccaaagacg ggctccgagc aaaccgagaa
     2641 aaagaagaaa aatattcgcc acgaagaggc tcgagaagag cgtgtacacc acctcaggat
     2701 ggcgggtgaa aagctccggt cccggctgga gatcatgaat catcagagcg ccgatgagca
     2761 cggcggctga ggaacttccc ggaatgccca atgtgagcag gggaacaaga gccccgccca
     2821 ccgaggcgct gttggaggcc tctgcggcgg cgacgcctgc gggagcgcct tttccgaaac
     2881 tctcaggttc tttactgagg cgcttctcga cgtcgtaggc gatgaaggag gcaatcgttc
     2941 cgcccgcccc ggggaagact ccgatgaccg tacccagaac ccctgcacga atcatggagt
     3001 atttcagctt caggtattcg cgtaccgtcg gccaggggga acgctccttg tcgggctgca
     3061 cggggggtat accgacctct cccgattcga tcccggaaaa gacctcgctg agagcaaaaa
     3121 ggcctatcag caccggaatg aaggagaaac cgtcgtacaa gcggaccacg tcgaacgtaa
     3181 agcggcttac accgctgaga gggtcgagcc cgatggtatt caggagcagc cccagaagca
     3241 ccgccatgaa ggactcaagc cagcgtcctc ctcccaaaga ggctatcgtg gagagtccga
     3301 gaattgccag agcaaagtac tccgcaggcc agaacttcag cgccatacgc gccaaggggg
     3361 ccgagaagag caccaggatg atgattccga taaatcctcc ggcaacagaa gcgacaagag
     3421 aaacgccgag cgcggttccc gcatcgcctt ttcgtgtcag aggatagccg tcaaaggcgg
     3481 taaccacagc cgagggcgtc cccggcgtgt tcaccatgac ggcggtgatc gacccgccgt
     3541 agttggaggc aaggtagata gcgcaaagca ggacgagccc catcgagggg gtcattccaa
     3601 aggaaaatgg cagaagcaac gcgacggcca tggagggaga gatgccgggc atggcgcctg
     3661 caagaatgcc gatcgccgcg cccgccataa ttacaagcaa aggagtaatt cccatgagcg
     3721 ctccgagtcc gtcaagcaaa tgggagaaaa gatccattcg acgttccccc tatccgaaat
     3781 agatgcccgc aggaagcgag agatgcagca ggcgaataaa gacaaaccag gagaagagca
     3841 tccagccccc tgtcagtgaa agaaggataa acaaccggcg ttctccaaag agcagcagaa
     3901 ggagaagaat cattccgccc gaggagatca ggtaaccgac gatctccatc ccccaaagag
     3961 agaaagcgac aatggccgca gccagcagaa cccgttgtac gtcaccggtc ttcggatcct
     4021 caggagcctt ccccaggagc gtgcgtcgca gctgatccag agtaaaaaaa gccagtcctg
     4081 cagcccagag cctgggcgcc gttctggcgc tgcttccgac ggcttcgatc tcctcgagac
     4141 cgaatgagag aagaaagaaa gcgaatgcaa ggaaaagcaa cgacacgcag atgattatac
     4201 gccccctgaa cggcgcgtgc ttcccacgtg tcaggaaggc cgtcagcgct atgacggcaa
     4261 gaaacgaaag aatccagacg gggaaactgt cggccccgct gagaaaaaga agcagagttt
     4321 ccatggaacc acct
//
LOCUS       DAOSWW010000095         4330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_13862_length_4330_cov_3.768655, whole genome shotgun sequence.
ACCESSION   DAOSWW010000095 DAOSWW010000000
VERSION     DAOSWW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13862_length_4330_cov_3.768655"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PK442_03040"
     CDS             complement(<1..671)
                     /locus_tag="PK442_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO86654.1"
                     /translation="MSGSLKIKLTLLDNIIWLMLFLFFFACALGIPAFASWNNIVNIL
                     YHTTIMSMLVLAQGFVLLNGNFDLSIDAVLAFAPGVVILASTKWFPVALGNPYLAILL
                     TLLLGGCIGFFNGYCVAKLGMNAFMQTLSLSIILRGMVLFLIPLSIFPLDPVYSFIGK
                     ARFSAVGNLPAAIPVTFTIYFLFHILLRHTVFGRNYLATGGNARASFVAGIDTRKMVI
                     AGFVIA"
     gene            complement(656..2203)
                     /locus_tag="PK442_03045"
     CDS             complement(656..2203)
                     /locus_tag="PK442_03045"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO86655.1"
                     /translation="MEEVCSKPVLLLEGISKVYPGTVALENIHFEALPGEVHGLIGKN
                     GAGKSTLVGILSGLVEPTSGTIMVNGRSFSSFSRSRAKREGISIVPQEPEIVLDLSVA
                     ENLFLGELENCSGFVSWSKIRADAQRILDRFGLDIGADLQARDLSLSERQILLILKAC
                     VVEDANVVVLDEASASLSRNDAAILKRIVGELRESGKAVLYISHHIDELLDICDRLTV
                     LRDGRIVTTRTKNMLDHHSLAELIVGETLSSSGPERKEAGYIGEELLRLENLTRWGNF
                     CNISFSLKKGEILGIAGLRGSGRTELLKAISGIEPADAGKIFFKDKRVSFSTPSEAIR
                     MGVAYLPEDRETEGLIQTFSIRENLLLNSISRFSRNGFVLRREAGRRAEEIFAGVQIQ
                     AFSMEQSAEELSGGNKQKVVIGRIMANAPEVYLLDEPTRGVDIGAKRSILSIIHGPLR
                     AASGVLLTSPGLDDLMDICDRILVLYKGNMVREYQQKDFDEKSLFMDVQGFGYQDQRG
                     GDECRAV"
     gene            complement(2203..3462)
                     /gene="hisD"
                     /locus_tag="PK442_03050"
     CDS             complement(2203..3462)
                     /gene="hisD"
                     /locus_tag="PK442_03050"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOO86656.1"
                     /translation="MIVLKEAERNFQEEGGSVESVVGGILKSVRERGDEAVLAYTEKF
                     DGVRPSSLRIGKEEMRSAYEKVSPSTVKTLEFAAERIRNFALRQKSCMQELRYEISPG
                     VTLGHRLLPMESCGCYVPAGRYPLPSSALMSAIPAKVAGVRRIAACSPAGKGRDGIHP
                     AVLVAMDIAGVDEVYCMGGAQAIGAFAYGTETIKGVDIIVGPGNRYVTEAKRQVSGAV
                     GIDMLAGPSEVVILADESANPEWVAADALARCEHDPHSWTVLVTTSRRLGESVMSAVE
                     TQAPLLSTGEFAFQSWRNNGRVLLAESLVEAVAITNDIAPEHLQVMTSDSPAISSQLV
                     NFGSLFIGENAPVVFGDYASGPNHILPTVRCARFSNGVYVGTFLRVCSFQELTAEGAA
                     YLAGPCADFAEMEGLFGHKRSAEMRAR"
     gene            complement(3565..>4330)
                     /locus_tag="PK442_03055"
     CDS             complement(3565..>4330)
                     /locus_tag="PK442_03055"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOO86657.1"
                     /translation="TFYDRFSVAGKVAVVTGAARGLGKGYALGLASAGATVLCGDVNE
                     NDAKLTAEEIRAFGGRAEGFFLDVTQPGKVEAVFSDAASRLGTLDIVVNNAGVEEIND
                     FVSVTEEQYDKIMSVNLRGVFFTAQAAAKIMIKQRRGKIVNIGSLGSAIGLAQSSVYC
                     GAKGGVVQITRTMAIELAKYNIQVNAMGPGYFRTPMTEPFFQNPEHRRWIEERIPAGR
                     VGTAEDLLGTLVFLCSEASDYLTGQIVYIDGGWLAS"
BASE COUNT         1038 a         1244 c         1089 g          959 t
ORIGIN      
        1 gctatgacaa aacctgctat caccattttt cgtgtatcta tacctgcgac gaagctggcc
       61 cgtgcatttc ccccagttgc cagatagttt ctgccgaaga cggtatggcg tagcagaatg
      121 tgaaagagga agtagattgt aaatgtcaca ggtatagccg cagggaggtt tccaacggct
      181 gaaaaacgcg ccttgccgat aaaggaatag accggatcca acggaaatat cgaaagggga
      241 ataagaaaaa ggaccattcc ccgaagaata attgaaagag aaagcgtctg cataaaggcg
      301 ttcattccca gcttggcaac acagtagccg ttgaaaaaac cgatgcaccc gcccaggagc
      361 agcgtgagga gaatcgccag gtacggatta cccaacgcga cgggaaacca cttcgtcgag
      421 gccaaaataa ctactccggg cgcaaaggcg agcacagcgt cgatagaaag gtcaaaattg
      481 ccgtttaaga gaacgaaacc ttgtgcaagc acaagcatgc tcattatcgt ggtatggtac
      541 agtatgttca caatgttgtt ccatgaggcg aatgccggaa ttcccagggc gcacgcgaaa
      601 aaaaagagaa agagcatcag ccagatgata ttgtccaaaa gagttaactt gatttttaaa
      661 ctgcccgaca ttcgtcgcca cctctttgat cttgatatcc aaaaccttgc acatccataa
      721 aaagagattt ttcatcgaaa tctttttgtt gatattcgcg aaccatgttc cccttgtaca
      781 gaacaaggat tctgtcgcag atatccatca gatcgtccag tcccggcgac gtgagcagta
      841 cgccggaggc ggcgcgcaac ggtccgtgaa ttatggagag gatggaacgc ttcgctccaa
      901 tatccactcc tcgcgtcggt tcgtcgagga gatagacctc cggtgcgttc gccataatac
      961 gtccaattac gaccttctgc ttatttcctc cggaaagttc ctcggcgctc tgttccatgg
     1021 aaaacgcttg aatctgtacc ccagcgaaga tctcttcagc gcggcggccg gcttctcttc
     1081 tcaaaacgaa gccattccgg gagaatctgg aaatgctgtt gagcagcaaa ttttcgcgga
     1141 tgctgaacgt ttgtatcagc ccctccgtct cgcgatcctc cggaagatac gctactccca
     1201 tgcgaatggc ttccgagggg gtggagaaag aaactctttt gtctttgaag aaaatttttc
     1261 ctgcatcggc aggctctatc cccgagatcg ccttcagcag ctcggtccgt ccgctccccc
     1321 gcaatccggc gataccgaga atttctcctt ttttgaggga aaagctgata ttacaaaaat
     1381 tcccccatcg tgtaagattt tcgagacgaa ggagctcttc gccgatatag cctgcttcct
     1441 ttcgttcggg gcccgaagaa gaaagcgtct ctccaactat cagttcagcg agcgaatgat
     1501 ggtcaagcat attcttcgtc cgtgttgtga cgatacgtcc gtcccgaagt acggtaagtc
     1561 gatcgcaaat atcgagcagt tcgtcgatat gatgggagat gtagaggact gccttccctg
     1621 actccctcaa ttcacccaca attcttttca gaattgcggc gtcgttccgg ctgagcgacg
     1681 ccgatgcttc gtcgagcaca acaacgtttg cgtcctcgac gacgcatgct ttcagaataa
     1741 ggagtatctg ccgctcactg agagagagat ctctcgcctg aagatcagcg ccgatatcga
     1801 gtccgaaacg atcaagaatg cgctgggcat ccgcccggat cttgctccat gaaacaaaac
     1861 cggaacagtt ttccagctct cccaggaaga gattctctgc aaccgaaaga tcaagcacta
     1921 tctccggttc ctgtggaaca atggagatcc cctcacgttt tgctcgcgat ctgctgaacg
     1981 atgaaaagga tcgtccattt accatgatgg ttccggaggt cggctccaca aggcccgaga
     2041 gaataccgac gagcgttgac ttcccggctc cgttcttccc gatgagaccg tgcacttctc
     2101 ccggaagcgc ttcgaaatga atattctcaa gagccactgt tcccggatag accttgctga
     2161 tcccctcaag cagcaaaact ggtttggagc agacttcttc catcaccgtg cacgcatctc
     2221 cgctgaacgc ttgtgtccga aaaggccttc catttcggcg aagtcggcgc atggtcccgc
     2281 aaggtaagcg gctccttccg cagtgagctc ctggaaggaa cagactctca ggaaagtccc
     2341 gacgtagacg ccgttcgaaa agcgggcgca cctgacggta ggcaaaatat gattcggtcc
     2401 cgaggcatag tcgccgaaaa cgaccggtgc gttctccccg atgaagaggg aaccgaagtt
     2461 taccagttgt gacgaaattg ccgggctgtc ggacgtcatc acttgcaggt gttcgggagc
     2521 gatgtcgttc gtaatagcca ccgcctcgac cagagactct gcaagcagca cgcgcccgtt
     2581 gttcctccag ctttggaaag cgaattcccc ggtggaaagg agcggcgcct gcgtttcgac
     2641 ggcgctcata acggattcac cgagccgcct gcttgtcgtc acaagtacgg tccacgaatg
     2701 ggggtcgtgc tcgcagcgcg caagagcgtc ggcggcaacc cactccgggt tcgccgattc
     2761 gtcggcgagg atcaccactt cgcttgggcc tgcaagcata tcgatcccta ctgccccgga
     2821 aacctgtcgt tttgcctcgg taacatacct gtttccaggg ccgacgatta tgtcaacacc
     2881 tttgatcgtt tcggttccgt aggcgaaagc gcctatagcc tgcgcgccac ccatacagta
     2941 gacctcgtcc actcctgcga tatccattgc gacaagaact gccgggtgaa ttccgtcccg
     3001 tcctttgccc gccggagaac acgccgctat ccgccgaact ccggcaacct tcgccggaat
     3061 agccgacatc aacgcggaag acggaagagg gtatctcccc gcaggaacat aacaaccgca
     3121 ggattccatg gggagaagcc gatgcccgag cgtcactccg ggagaaatct cgtaccgaag
     3181 ctcctgcata cagcttttct ggcgaagtgc aaagttgcgg attcgttccg cggcaaattc
     3241 aagcgtcttc accgtcgaag gggatacttt ttcataggcg gagcgcatct cctctttccc
     3301 tatacgaaga gaggaaggac ggacgccgtc gaacttctcc gtgtaggcga gaacagcctc
     3361 gtctccgcgt tcgcggacac tcttgagaat tccgcctact acggattcaa cagagcctcc
     3421 ttcttcctga aaattacgtt ctgcttcttt gagtacgatc acttcacatt cctcctgatc
     3481 ggtctggaaa ctcccgtcta ttggagcggg agcaaaaaga gagagccgag aagatgttca
     3541 tctctcctgt acattacaat ttcctcagct tgcgagccag ccgccgtcta tgtagactat
     3601 ctgtcccgtc aaataatcgg aggcttcact gcacagaaag accagcgttc ccagaagatc
     3661 ctcggcagtc ccgactcgcc ccgccggaat ccgctcctcg atccaccggc ggtgctcagg
     3721 gttctgaaag aacggctcgg tcatgggagt acggaagtac ccgggcccca tagcgttcac
     3781 ctggatgttg tacttcgcca gctcgatagc catcgtgcgg gtgatctgca ccactccgcc
     3841 cttggcgccg caatagaccg aggattgcgc cagtccgatg gcgctcccca gcgacccgat
     3901 attgacaatt ttgccgcgcc gctgcttgat catgatttta gcggctgcct gcgccgtgaa
     3961 gaagacgccc ctgagattga ccgacataat cttgtcgtac tgctcctccg taacggagac
     4021 gaaatcgttg atctcctcga cgcccgcgtt gtttacaacg atatcaagag ttccaagacg
     4081 ggaggctgcg tcggaaaata cagcttccac ttttcccggc tgcgtcacat cgagaaaaaa
     4141 gccctctgcc cggcccccga aggctcgtat ctcttcagcc gtcaatttcg catcgttttc
     4201 gttcacatcg ccgcacaata cggtcgcgcc ggcagacgct aatccgagcg catacccctt
     4261 cccgagtccc cgggctgctc cagtaacaac ggcgactttc ccggccacgc tgaatcgatc
     4321 ataaaaagtc
//
LOCUS       DAOSWW010000096         4330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_13863_length_4330_cov_3.704795, whole genome shotgun sequence.
ACCESSION   DAOSWW010000096 DAOSWW010000000
VERSION     DAOSWW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13863_length_4330_cov_3.704795"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1182
                     /locus_tag="PK442_03060"
                     /pseudo
     CDS             154..1182
                     /locus_tag="PK442_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
     gene            1237..2748
                     /locus_tag="PK442_03065"
     CDS             1237..2748
                     /locus_tag="PK442_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HOO86658.1"
                     /translation="MQVKKDNSVFFISMAVTAATVLWGIFSPASFDAAAKGLFNVLIE
                     YFGWGYMLAMNIFVLFPILLCMSRFGKLRLGEPGSKPEFGNISWFAMLFSAGMGVGLV
                     FYGVGEPVYHFMTPPFGAAAGSAKAAEDALRTTFFHWGLHPWAGYAVIALCLAFFQYR
                     KGAPGLMSSMFLPVLGEKGNASMLGKTIDILAIFATIAGLATSLGLGTLQINSGLKYL
                     FNAPQNVTFQLVIIATLAFVYTGTAVTGIEKGIKFISNVNLFLACLLSVSLFFIGPTM
                     AIIESLMTGIGDYLSNVVNQSFHMAPYGGEYKGWLGGWTLYYWAWWIAWAPFVGSFIA
                     RISRGRTIREFVSGVLVVPALGSFTWFAIFGTSGLNLELTGAAKIAEKVAADISIGVF
                     EVYTHYPLGGIMSIIMVALISTFFITSANSATFVLSMYSSQGDMNPPKFRMGIWGVLM
                     AALAFVLLMSGGLQSLQIASIAAAAPFAVIMVIACWCLWKALKKDEATFTELN"
     gene            2910..3896
                     /locus_tag="PK442_03070"
     CDS             2910..3896
                     /locus_tag="PK442_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86659.1"
                     /translation="MEYGSLLLLFDEENPCWTEELVRTAEEDPGPLALMLGEGLLAKE
                     GELFFLTGAGREAFSEEAAANYLPAVPGIPGADKEKDLFRTRLRLLLDKKHIQRWGVK
                     EFVLSARFPTPPLQDDELFTRSSVFEWIWPSSPIIGQMRDDWPVTGLAARKETPPPPD
                     VAAKWFEAKGSYPAYFEADLLHLSRYDFQAYAHIPRSLNDPWGILNADRFFCMRAQAP
                     QTLNPDGFLATIGRFQLALEVLRRMVLPGYIDLDSFDQDGINWLLFLFEREEEAEACV
                     SLLAPFGLDLIRGSMPMDVWALSFEALENFKSKAETIHDLIPVIGRPVVRTP"
     gene            4111..>4330
                     /locus_tag="PK442_03075"
     CDS             4111..>4330
                     /locus_tag="PK442_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOO86660.1"
                     /translation="MNSMFAARMERLAEELRRREADALFVFSSELDNRPAVQYLSGFT
                     GSFAVVVAGVNGGRLVTDSRYFIQAEEES"
BASE COUNT          799 a         1427 c         1300 g          804 t
ORIGIN      
        1 tgaggtcgag gccttgaccc ggagaacggg cgatcccctg acctcccggg tcgccgccca
       61 gctggtggag ctgctcaatg gttctggcga ggaggctgct cttgcccaca ttgctctgcg
      121 ggaactccac gccgccgtca cggcctcgcc gcccgcctcg tcggcctgtg gctgcgcgtc
      181 cccgtgctca cctcgatgaa cgaggaaaac cccggcgtcg agctgttcca caaagacatg
      241 tacatcttcc gcggcggcaa ccgcgccacg ttcatgaagc aggacatgga gaagctcgcc
      301 gccttcgccg acaaaatcgc gcggggcggg aagctgcttc ccgcgccgat ggaggggtac
      361 ttcccgcgcg gcatccgcca cgaagtcttc cttgaggata tcgggcaaga gccggtcatg
      421 gccgccgacc gcgtggtcga catgatgatc gcgaagctga agggcgaacc gtatcagaca
      481 gagctgccaa tgcccgcgct cgaccgcgtc cccgtcgctc cggcggtgaa ggatctgcgg
      541 aaggtgcgcc ttgcgctggt cacctccggc ggcatcgttc cccgcgagaa ccccgaccgg
      601 attcaatcct gctccgccac caagtggggc aagtacgaca tctcggcgct cgacaagggc
      661 ttcaccgcgc ccgagtacaa gaccatccac gccgggtacg accccgagca ggccgacaag
      721 aaccccaacg tcgtcgtccc gctcgacgcc atccgcgcct accagcgcga gggcaggctg
      781 ggcgtggtgg acgagttctt ctacaccacc gtcggcacgg gtaccacgca gggcgaggcc
      841 gcccgcatgg gccgtgagat cgccgcggtg ctccacgagc ggaacgtcga agccgtcgtt
      901 ctcaccgcga cctgaggaac ctgcactcgt tgcgggtcaa cgatcgcgaa agagatagag
      961 cgcagcggca tcccggtggt tgtgatgtgc aacctcacga atatcgcaaa gaccgtcggc
     1021 gtcaacaaga tggtgcccac ggtctccgtc ccgtaccccc tcggcaaccc ccagctccgc
     1081 cccgacgacg aatgggccct ccgctaccac aggacgggcg tggcgctcga cgcgctggaa
     1141 accgacgtcg acggccagac catcttcccg gtgaagatct agcttccgtc cgggggagga
     1201 cctcctcccc cggatatcag tgaaggaggg acgagtatgc aagttaaaaa agataattcc
     1261 gtgttcttca tctccatggc cgtcacagcc gccaccgtgc tctgggggat cttctccccg
     1321 gcgagcttcg acgcggcggc caaggggctt ttcaatgtcc tcatcgagta cttcggttgg
     1381 gggtatatgc tcgccatgaa catcttcgtg ctcttcccca tactcctctg catgagccgc
     1441 ttcggaaagc tccgcctcgg cgaacccggg tcgaagccgg aattcggcaa catctcgtgg
     1501 ttcgccatgc tcttctccgc gggcatgggc gtcggcctcg tcttctacgg cgtcggcgaa
     1561 cccgtctacc acttcatgac gcctcctttc ggcgcggccg ccggaagcgc gaaagccgcc
     1621 gaggacgcgc tgcggaccac cttcttccat tggggcctcc atccgtgggc ggggtacgcc
     1681 gtcatcgcgc tctgcctggc cttcttccag taccgcaagg gcgctcccgg actgatgagc
     1741 agcatgttcc tccccgtgct cggggaaaag gggaatgcga gcatgctcgg caagaccatc
     1801 gacattctcg ccatcttcgc caccatcgcc gggcttgcca catcgctcgg attgggcacg
     1861 ctccagatca acagcggcct gaagtacctc ttcaacgcgc cgcagaacgt caccttccag
     1921 ctcgtcatca tcgcgacgct tgcgtttgtg tacaccggca cggcagtcac cggcatcgag
     1981 aaaggaatca agttcatctc caacgtcaat ctctttctcg cctgcctgct gagcgtctcg
     2041 ctcttcttca tcgggcccac gatggcgatc atcgaatcgc tgatgacggg catcggcgac
     2101 tacctctcca acgtggtgaa ccagagcttc cacatggcgc cctacggcgg cgagtacaag
     2161 gggtggctcg gcggctggac gctctactac tgggcatggt ggatcgcatg ggcgcccttc
     2221 gtcggctcgt tcatcgcccg catctcgcgc ggccgcacca tacgggagtt cgtctccggc
     2281 gtgctcgtcg tacctgcgct gggcagcttc acctggttcg ctatcttcgg gacttccggg
     2341 ctgaacctcg aactcaccgg cgcggcgaag atcgcggaga aggttgccgc ggacatctcc
     2401 atcggcgtct tcgaggtcta cacccactac ccgctgggcg gcatcatgtc gatcatcatg
     2461 gtggcgctga tttcgacctt cttcatcaca tcggccaact cggcgacgtt cgttctctcg
     2521 atgtactcca gccagggcga catgaacccg ccgaagttcc gcatgggcat ctggggcgtc
     2581 ctgatggcgg cgctcgcctt cgtcctgctg atgagcggcg gactccagtc gctgcagatc
     2641 gcctccatcg ccgctgcggc gcctttcgcg gtgatcatgg tgatcgcctg ctggtgcctc
     2701 tggaaggcgc tcaagaagga cgaggctacc ttcaccgaac tgaactgacc tctggacgaa
     2761 ccggccgttt cgcgctacgc ttccgaaggg gaatgcgtac ggctgaagag aaagaggaga
     2821 aaaacgcgtc tcgacgcgcc cctggcatgc tccgccatcc gggggcgttt tttgtacgaa
     2881 aagagaagat tcgagaggag gaagaacaca tggagtacgg aagccttctg ctattgttcg
     2941 acgaggaaaa cccctgctgg acggaggagc tcgttcgtac cgccgaagag gatccagggc
     3001 cgctcgcact gatgctcggc gaagggctgc tcgcaaagga gggcgagctc ttcttcctga
     3061 ccggcgcggg gagagaagcc ttcagcgagg aggcggcggc gaactacctg cccgccgtac
     3121 cgggaattcc cggagcggac aaggaaaagg accttttcag aacgcgcctc cgcctgctgc
     3181 tcgacaagaa acacatccag cgctggggtg tgaaggagtt tgtcctgtcc gcgcgcttcc
     3241 cgaccccgcc gctgcaggac gacgagctct tcacccgctc gtccgttttc gaatggatct
     3301 ggccctcttc gccgattatc ggacagatgc gcgacgactg gcctgtcacg ggactcgccg
     3361 cccgaaagga aacgccgcct cctccggacg tcgccgcaaa gtggttcgag gcgaagggaa
     3421 gctacccggc gtacttcgag gcggacctgc tccacctcag ccgctacgac ttccaggcgt
     3481 acgcgcacat cccccgctcc ctcaacgacc cgtggggaat actgaacgcg gaccgcttct
     3541 tctgcatgcg ggcgcaggcg cctcagacgt tgaaccctga cggatttctc gcgacgatag
     3601 gacgctttca gctcgcgctc gaagtgctcc gccgcatggt gctccccggc tatatcgacc
     3661 tcgacagctt cgaccaggac ggcatcaact ggctgctctt cctcttcgag cgcgaggagg
     3721 aggccgaagc gtgcgtctcg cttctcgccc cgttcgggct cgacctgata cgcggctcca
     3781 tgccgatgga cgtctgggcg ctcagcttcg aggcgctgga aaactttaag tctaaggcgg
     3841 agacgatcca cgaccttatc ccggtcatcg ggcgccccgt cgtccggacc ccctgaagac
     3901 gaagaaaaac agtcgggaga gaagcgccga cgacgctcgt ccgaggcgtc tctcccccgg
     3961 agcacgctcc tgaggcttgt ccggacactt agttgacgaa agaacttttc tccgagtaaa
     4021 agaaaaccct ttcaccctca tcatcgactt ggtataatgc atactgttcc gtatcctatt
     4081 tcaaagaagg ggagcggtcg tgtgcgagag atgaacagta tgttcgcggc gcggatggag
     4141 cgcctggcgg aagaactccg gaggagagag gccgacgccc tcttcgtctt cagcagcgaa
     4201 ctggataacc gtcccgcggt gcagtacctt tcaggattca caggctcctt cgccgtggtt
     4261 gtggcggggg tgaacggcgg gcgtctggtc acggattcgc gctacttcat ccaggccgag
     4321 gaggaatcct
//
LOCUS       DAOSWW010000097         4256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14199_length_4256_cov_4.113782, whole genome shotgun sequence.
ACCESSION   DAOSWW010000097 DAOSWW010000000
VERSION     DAOSWW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14199_length_4256_cov_4.113782"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>4256
                     /locus_tag="PK442_03080"
     CDS             145..>4256
                     /locus_tag="PK442_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86661.1"
                     /translation="MRLQKMFNGEDTTSEAAAIGDLMIEYASELSGSYLTHMLGAGFG
                     SMRGVGGRAGKTIASKIKAGIAGSGLDKVAGAKAFGDTVGKTARRLLAGAAKTGETVR
                     GAFDNSDFMKMITAHGYNGVIGEMLEERFGGFLRGLTGLDGDEYHSYIANVWNATIPK
                     STDQFMAELVAFSLPAAANAVGVQTSKVMAGRADPEYDRFVRIGNLTETARALRRKKT
                     TNVPAPGKNAEGKKSESPIMEPTFDDVVESLVAIDRLHEAEQLRHKEGYGVFGWVLDA
                     VNAAARKVAGMQYITSTDAESLGLQKSGWRSSGYGAAMRAGIVSMANESHASLESDLK
                     TDPEYARLLEVAGRSQSQEAKQALRDRFDRLQRDQAIKTAVALQAYTGLMDLSSQFSP
                     AEIEELQAGGMSTDEVKGLSERLEKLREDPAFLDTLEWDQAAKRVRFTPLALLTLDSP
                     QLAKVLIRRNLTGFALDETSPVVREDVSGLLAALDMVLDADVDGSYQTGLDYAQRLRQ
                     YLSSKDGNANLLSLIVPELSPVLNPEMVRAGSSEAVGLQTGKEQLLELFDRLDPSGEA
                     RSRLHNVSRYTNPAFVSGALTFKAEELTPQDGVPTNPFQVVMSRLAGNDRNVDARTYA
                     TFMAINGFNPASASHRVDFEAGLRGVLTALELGSSPSELWRDKEVVARSRVDGRLKER
                     IGVVSKIKRAKDGAVERVEVTFGQAPAEEFDPKDLSPRSDVIPYVNFAFGPGSLYTTR
                     ANARLRYPDQYNQIKDVDPVRPGVDEDGTPNKDKDLLRLAFGATVGPGGSITVNLFSS
                     SHAVEDILESALRRYNEATGKKGDSPLRVPGFFLEKVRRVRAEFADRLKSANDNEESA
                     GLADDPLYQAFKEVAGAEEERAMLFELYGKLYKRHRLSFKEEIDARGKPVFHALDKAA
                     DRLAALLGEEGTRQLLEEQHAFYLRFMSPEALAGLDYNYRIEQAVEAATPTGQKQRRV
                     DYGPDAAVVYVERDAKGRVAQARPGRGRLVSKGMSGEKWGDAKIQNRLGTYETGFRSR
                     PGSRSVLEVRDVNAAVKSAHGRATAAGKRYAEAQKKAEKTRGEARATLREEYEGLRRK
                     RETLSAERKGAEETLARARADGDAAAESDATKKLVDVQIAYAKAGERLEQIVREEQRL
                     DEEGDSDTDLAAAKLAYDAALQELTAAVAENERALRPLVLDESGAERDEVDDDIPMGD
                     PPAPTRSVIPEGTTTVEQAEALAEAYSSRGEEVPEELSRFIDTLRAARESKPKPEQKP
                     EPESKPEPSKKKKKKPPRVRGRWSKFDGEGGQATTSMLDSGEERLREAARRDGQQADE
                     LEARMRAFKGAPPDRPVDFSALDKMFESKTKQAPAKAMIDALELGPEATAA"
BASE COUNT          890 a         1192 c         1519 g          655 t
ORIGIN      
        1 gtggcaaggg ggacgggaaa agtcgtcggc gcgctgacgg acatgctccc gcaggccacc
       61 atgggcgtgg cgcagaagac cgcgatcgag atgctcggga cagcggcgat gggagaggat
      121 cctaccacgg ccacgcatgc ccgcatgcgg ctgcagaaga tgttcaacgg ggaggacacg
      181 acgagcgagg cggccgcgat cggcgacctt atgatcgagt atgcgtccga gctctccggc
      241 agctacctta cccacatgct gggggcagga ttcggaagca tgcgcggagt cggcggaagg
      301 gccggcaaga cgatcgcatc caagataaag gccgggatcg cagggtccgg tcttgataag
      361 gtggccgggg cgaaggcctt cggcgacacg gtcggcaaga ccgccaggag actacttgcc
      421 ggggccgcga agacggggga gaccgtgcgg ggcgcgttcg acaactccga cttcatgaag
      481 atgattacgg cgcacgggta caacggtgtg atcggggaaa tgctcgaaga gcggttcggc
      541 gggttcctgc gcgggcttac cgggctcgac ggcgacgagt accactcgta catagcgaac
      601 gtgtggaacg ccacgatccc gaagagcacc gaccagttca tggccgaact ggtggcgttc
      661 tctctcccgg cggcggccaa cgccgtgggc gtgcagacct caaaagtcat ggccggcagg
      721 gccgaccccg agtacgatcg cttcgtcagg attggcaatc tgaccgagac cgctcgggcg
      781 ctccgtcgca agaagacgac gaacgtcccc gcccccggta aaaacgcaga gggcaagaaa
      841 tcagaatccc cgatcatgga gcccacgttc gacgacgtcg tggagagcct cgtcgcgatc
      901 gaccgcctgc acgaggcgga gcagctgcgg cataaagaag ggtacggcgt attcgggtgg
      961 gtgctggatg ccgtgaacgc ggctgcgcgc aaagtggcgg gcatgcagta catcacgagt
     1021 acagatgcgg agtcgcttgg tctgcagaag tccgggtgga ggtcttccgg ctacggcgcg
     1081 gcgatgcgcg ccggcatcgt gtcaatggct aacgagtccc atgcgtcttt ggagtccgat
     1141 ttgaagacgg acccggagta cgccaggctg ctggaagtgg cgggcaggag ccagtcccag
     1201 gaagccaagc aggcacttcg cgatcggttt gaccgcctgc aaagagacca ggctatcaag
     1261 accgccgtcg cgcttcaggc gtacacgggg ctcatggacc tgtcttccca gttctcgccg
     1321 gcggagatcg aggagctgca ggccgggggg atgtcgacag acgaggtgaa ggggttgtcg
     1381 gagcggcttg agaagctcag ggaggacccg gcgttcctgg acactttgga atgggatcag
     1441 gccgcgaagc gcgtgcggtt cactccgttg gcgttgctga cgctggattc cccccagctc
     1501 gccaaggtct tgatccgaag aaatttaacc gggtttgccc tggacgagac cagccccgtt
     1561 gttcgggagg acgtgtccgg actcctggcg gctttggaca tggtgctcga tgcagacgtc
     1621 gatgggtctt accagaccgg gctggattac gcgcagaggc taaggcagta tttgtcgtcg
     1681 aaagacggca atgcaaatct gttaagtctc atcgttcctg agctgtcccc cgtgctcaac
     1741 ccggagatgg tccgcgcggg gtcgagtgag gcagtggggc tgcagaccgg aaaagagcag
     1801 ttgcttgagc tgttcgatcg gctggaccct tcaggcgaag ctcgctcccg cctgcacaac
     1861 gtgtcgcggt acaccaaccc ggccttcgtg tccggggcgt tgactttcaa agcggaggag
     1921 ctgacgccgc aggacggcgt cccgacgaat ccgttccagg tcgtaatgtc acgacttgca
     1981 ggcaacgaca ggaacgtgga cgcccgcacg tacgcgacct tcatggcgat caacgggttc
     2041 aacccggcgt cggcttcaca cagagtggac ttcgaggccg ggcttcgcgg cgtcctgacg
     2101 gccttggaac tcgggtcttc cccgagcgag ctgtggaggg acaaagaggt ggtcgccagg
     2161 tcccgcgtgg acggccgtct gaaagagcgg atcggagtcg tgtccaagat caagcgggcg
     2221 aaggacggcg ccgtggagcg ggtcgaggtc acgttcggcc aagcgcctgc ggaagaattc
     2281 gatcccaaag acttatcccc tcggtcggac gtcataccat atgtcaattt cgcgttcggt
     2341 cccgggtctc tgtacacgac gcgggcgaac gcccgcctgc gctatcccga ccagtacaac
     2401 cagataaagg acgtggaccc ggtccgaccc ggagtggacg aagacggcac cccgaacaaa
     2461 gacaaggact tgcttcggct ggcgttcggg gcaactgtag ggccgggggg ctccataacg
     2521 gtcaacctgt tctcctcctc gcacgccgtt gaggatattc tggagtctgc gcttcggaga
     2581 tacaacgaag ccaccgggaa gaagggggac tccccgcttc gagtcccggg attctttttg
     2641 gaaaaggtgc ggcgcgtccg cgctgagttt gcagacaggt tgaaatccgc gaacgacaac
     2701 gaggagtcgg ccgggctcgc ggacgacccg ttgtaccagg cgttcaagga ggtggcgggg
     2761 gccgaggaag agcgggccat gctgttcgag ctgtacggca agctgtacaa gcggcaccgg
     2821 ctaagcttca aggaagaaat agatgcgcga gggaagcccg tgttccacgc cttggataag
     2881 gcggcggacc ggctggccgc gttgcttggt gaagagggga ctcggcagct gttggaggaa
     2941 cagcacgcgt tctaccttcg attcatgtcc ccggaggcgc ttgccggtct cgactacaac
     3001 taccgtatcg agcaggcggt ggaggcggcc acccccaccg gccagaagca aaggcgtgtc
     3061 gactatggcc cggacgccgc ggtggtctac gtggagcgcg acgcgaaggg acgggtcgcc
     3121 caggcccgcc caggacgcgg caggctcgta tcgaagggga tgtccggaga gaagtggggc
     3181 gatgcaaaaa tccaaaaccg cctgggcacc tacgaaacgg gttttcggag ccgcccgggc
     3241 agtcgcagcg tgttggaggt ccgcgacgta aacgccgcgg ttaaatctgc tcacgggcgg
     3301 gcgacggcgg ccggtaaacg atacgcggaa gcacagaaga aggcggagaa gacccgaggg
     3361 gaggcccggg cgactctgcg ggaggagtat gaggggctgc gcaggaaacg agagaccctg
     3421 agcgcggaac gaaaaggcgc ggaagagacg ttggccaggg cgcgggccga cggggacgcg
     3481 gccgccgaga gtgacgcgac gaaaaaattg gtcgacgtcc agattgcgta cgcgaaggcc
     3541 ggagagcggc tcgagcaaat cgttcgagaa gagcagcgcc tcgacgagga aggcgactcc
     3601 gacacggacc tcgcggccgc gaagctcgct tatgatgcgg cgttgcaaga gctgactgcc
     3661 gcggtggcgg aaaacgagcg cgcgctgcgg ccgctggtat tggacgagtc cggcgccgag
     3721 cgggacgaag tagatgacga cataccgatg ggggacccgc cggccccgac ccgtagcgtc
     3781 attccggaag ggactactac cgtagaacag gcggaggcgt tggcggaggc ctattcttcg
     3841 agaggggaag aagttccgga agagctttct cggtttattg acaccctgcg ggctgcgagg
     3901 gagtcgaagc cgaagccgga gcagaagccc gagccggagt cgaagccgga gccgagtaag
     3961 aagaaaaaga agaagccccc tagagtgagg ggccggtggt caaaatttga tggcgagggg
     4021 ggacaggcaa ccacctccat gctggatagc ggggaggagc ggctgcgcga agcggcccgc
     4081 agagacggcc agcaggccga cgagctggag gctcgtatgc gagcgttcaa gggggctccg
     4141 ccggaccgcc ccgtggattt ctctgcgcta gacaagatgt tcgagtcgaa gaccaagcag
     4201 gctccggcca aagccatgat agacgctttg gagctggggc cagaggcgac ggccgc
//
LOCUS       DAOSWW010000098         4255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14204_length_4255_cov_4.010000, whole genome shotgun sequence.
ACCESSION   DAOSWW010000098 DAOSWW010000000
VERSION     DAOSWW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14204_length_4255_cov_4.010000"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PK442_03085"
     CDS             <1..581
                     /locus_tag="PK442_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO86662.1"
                     /translation="ASSMEPILDGFGLENGPVRVEKGCIAVDESMMTSVEGVYAVGDC
                     TGGAMLAHLAEYQALAAVSHMTGGSYRVDLDALPACVFIEPEVATVGLTEEEARARGE
                     EIVSAKAHFGANGMALAMGEGDGFVKVVARAADRTILGVHIMGPEAASLLGEATLAVA
                     KHLTAEDVANTIHAHPTLCECFRDACRRVMEE"
     gene            complement(729..1844)
                     /locus_tag="PK442_03090"
     CDS             complement(729..1844)
                     /locus_tag="PK442_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86663.1"
                     /translation="MASEQAESKGKIMIVDDTVVNLELLQNLLQRRGYSIMAFPQGKL
                     ALKAALKSPPDMLLLDINMPEMDGFEVCSHFKSEPSLAKIPVLFISALEASQDKVRAF
                     AAGGVDYVTKPFQIEELNARVDTHLKIVRMQRELERYNHELEELVQEKVRDIEESHLS
                     TILAMVKLTEYRDVGTGMHIERTRTLCSMLAAKLAEHPEYDGLLSPEYAETIYNAAPL
                     HDVGKVGVPDGILLKPGRLTDDEWEIMKTHSEIGAETLREVRRKYVSNAFVNMGIEIA
                     LFHHEKWDGSGYPEGRSGEAIPLSARIMAVADVYDALRTRRPYKEPFSHEESFAILQK
                     DSGSHFDPLLVEVFAQLEDEIKDLYNRCADLDGSSCR"
     gene            complement(1834..>4255)
                     /locus_tag="PK442_03095"
     CDS             complement(1834..>4255)
                     /locus_tag="PK442_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86664.1"
                     /translation="VSGESVGEFAIMTDITADRNHLRRQVAMMWGIGGTIAAALLILL
                     FTLLRRVDAGILAQQKALRESDALLKKLSRQIPGVLYQYRQEPGGPIGLLFVSDNLTD
                     VCGLEPDEVIRDIGSFFALVHPEDREGLEERLAASSRTMEVWEHEFRVVLPEKGERWL
                     FTIGNPERLPDGSVLMHGYVVDSTERREREDEVRKQAGLIASMLNSIPDIIFYKNTDG
                     VFLGCNRPHEEMTGRSESETVGKTDYDFYDRGIADFYREKDKEALAAERPLHNEEWVT
                     YPDGRRALLDTLKTSFRGKDGELIGLLGISRDITERKRMEEDLRAALEAANAASLAKS
                     AFLASMSHEIRTPMNAILGFSQLLERDPELAPKHMKYVRIINRSGEHLLALINDILDM
                     AKIESGRMTLNRAPFSPFAMLRDIVSLFHTLAEEKSLSLTIEHDDDLRAATLLGDEGK
                     LRQVLVNLVGNGLKFTQQGSVTVRFRTETDFRDASSDERELLLSVEVEDSGVGISPEQ
                     IELIFAPFQQSDSGKKAGGTGLGLTISRKFVEMMGGTLTCESELGKGSCFRFRIPVER
                     VYDVSLRPSQPGGRVLGLKPESGRPLILIADDDDTSRGLLTELLTEAGFDCAEAANGL
                     QAVELCASRTPDAVLMDMNMPLLDGYEATRMIKESDSRNIPVIALTADAFARDAVAAC
                     GADEYLRKPFVADELFAVLQTVLGLEYIYEDVSGRDTAGGEAGSEAGGPGSPKAIDLS
                     LIPGNLLRELHGAVEDGDMYRIRELIRSISAVDEKVGKELLLLAERYDYERLLELVND
                     EGGLSDGE"
BASE COUNT          756 a         1416 c         1228 g          855 t
ORIGIN      
        1 tggcctccag catggagccg atcctcgacg gattcgggct ggagaacggc cccgtccgcg
       61 tggagaaagg gtgcatcgcc gtcgacgagt ccatgatgac gtcggtcgag ggggtctacg
      121 ccgtcggcga ctgcacgggc ggagccatgc tggcccacct cgcggagtac caggcgctcg
      181 ccgccgtgag ccacatgaca ggcggttcgt accgggtgga cctcgacgcc cttccggcgt
      241 gcgtctttat cgagccggag gtcgcgacgg tcggtctcac cgaggaggag gcccgcgcgc
      301 gcggcgaaga gatcgtctcc gcgaaggccc acttcggcgc aaacggcatg gcgctcgcca
      361 tgggcgaggg ggacggcttc gtcaaggtgg tcgcccgcgc agccgacagg acgatcctcg
      421 gagttcacat catggggccg gaggccgcct ccctcctggg agaggccacg cttgcggtcg
      481 cgaaacacct gaccgccgag gacgtcgcga acacaatcca cgcccacccc accctgtgcg
      541 aatgtttcag ggacgcctgc cgccgcgtaa tggaggagta ggcgtcctcc gcagaaacgt
      601 catcgccgga gcggaatctc ctgttccttc acatacacac aacgcggccc ttcgcaagag
      661 tgcgaggggc cgcgtgctgt ctcaccgagc gccgggactt caatagcgag cgccgcttcc
      721 cggcggcgtc agcggcagga agatccgtcg agatcggcgc agcgattgta gaggtccttg
      781 atttcgtcct ccagctgcgc gaacacctcg accagcagcg ggtcgaaatg actccccgaa
      841 tccttttgca ggatggcgaa actttcctcg tgagagaagg gctccttgta cggacgccgc
      901 gtccggagcg cgtcgtagac gtcggcgacg gccatgatgc gcgcgctcag tggaatagcc
      961 tcccccgagc gcccttcggg gtagccgctg ccgtcccact tctcatgatg gaaaagagcg
     1021 atttctatcc ccatattcac gaacgcgttc gagacgtact tcctccgcac ctcccgaagc
     1081 gtctccgcgc cgatctcgga gtgcgtcttc atgatctccc actcgtcgtc cgtcagccgc
     1141 cccggtttca gcaggatgcc gtccggaacg ccgaccttgc cgacgtcgtg cagcggagca
     1201 gcgttgtaga tggtctccgc gtactccggg gagagaagac cgtcgtactc cggatgctcc
     1261 gcgagcttcg cggcaagcat actgcacagg gtccgcgtgc gctcgatgtg cattccggtg
     1321 ccgacgtccc ggtactccgt cagcttcacc atcgccagta tcgtggagag gtgcgactct
     1381 tcgatgtccc tgaccttctc ctgcaccagt tcctccagct catgattgta gcgttcgagc
     1441 tcgcgctgca tccggacgat cttcaggtgc gtatcgacgc gcgcgttcag ctcctcgatc
     1501 tggaagggtt tggtcacata atccaccccg cccgccgcaa acgcgcgcac cttgtcctgg
     1561 gaggcttcga gcgcgctgat aaagagcacg ggaattttcg ccagcgaggg ctccgacttg
     1621 aagtggctgc acacctcgaa accgtccatc tcgggcatgt tgatgtccag caggagcata
     1681 tccggaggac tcttcagggc cgccttcaac gcaagcttcc cctgaggaaa cgccatgatg
     1741 ctgtaccctc tgcgttgcag caggttttgc agcagttcga ggttcacgac ggtatcgtcg
     1801 acgatcatta tcttcccttt gctttcagcc tgttcactcg ccatcggaga gtcctccttc
     1861 atcgttcacc agctccagca gtctctcgta gtcgtaccgc tcggcgagga gaagcagctc
     1921 cttccccacc ttttcgtcca ctgcggatat gctccggatc aactccctga tgcggtacat
     1981 atcgccgtct tccaccgcgc cgtgcaattc tcgcagaaga ttccccggaa tcagggaaag
     2041 atcgattgct ttcggggagc ccgggccgcc ggcttccgac ccggcctcgc ccccagccgt
     2101 atctctgccg gatacatctt cgtagatata ttccagtccg agaaccgtct gcagtacggc
     2161 gaagagctcg tccgccacga agggcttgcg cagatactcg tcggcgccac acgctgcgac
     2221 ggcgtcgcgg gcaaaggcgt cggcggtaag cgcgattaca gggatattcc tgctgtcgct
     2281 ctctttgatc atcctcgtgg cttcgtaccc gtcgaggagc ggcatattca tatccatcag
     2341 cacagcgtcc ggcgtgcggg atgcgcacaa ttcgacggcc tggagaccgt tcgccgcctc
     2401 ggcgcaatcg aatcccgctt cggtgagaag ttcggtcaaa agtccgcggc ttgtatcgtc
     2461 gtcgtccgcg atcagaataa ggggacgccc gctttccggt ttcagcccca gtacgcgccc
     2521 gccgggctgc gacggccgca gggaaacgtc gtacaccctc tccaccggta tccggaagcg
     2581 gaaacacgac cccttgccca gttcgctctc gcaggtcagg gttccgccca tcatctccac
     2641 gaacttccgg ctgatggtga ggccaagtcc ggtgccgccc gccttctttc cggagtcgct
     2701 ctgctggaac ggcgcgaaaa tgagttcgat ctgctccgga gagatgccga cgccgctgtc
     2761 ctcaacctcc accgagagca ggagttcgcg ctcatccgag gaagcatccc ggaaatcggt
     2821 ttccgtgcgg aaacgaacgg tcacgcttcc ctgttgcgtg aacttgagac cgtttcccac
     2881 aagattgacg agcacctgcc gcagttttcc ctcgtcgccc agaagcgtcg ccgcgcgcag
     2941 atcgtcgtcg tgttcgatcg tcagggagag cgacttctct tccgccagcg tatggaagag
     3001 cgagacgata tcccgcagca tggcgaacgg gctgaacggc gccctgttca aggtcatcct
     3061 gccggattct atcttcgcca tgtcgagaat gtcgttgatc agcgcgagca ggtgctctcc
     3121 gctgcgattg atgatccgca cgtacttcat gtgcttcgga gcaagctccg ggtcgcgctc
     3181 caggagctgc gagaagccca gaatcgcgtt catcggcgtc cggatttcat ggctcatgct
     3241 cgccaggaag gcgctcttcg ccaggctcgc ggcgttcgcc gcctcaaggg ctgcgcgcag
     3301 atcctcctcc atccgtttgc gttcggtgat atcgcggctg atcccgagga gaccgatcag
     3361 ctcgccgtcc ttcccccgga aggacgtttt gagcgtgtcg agaagcgcgc gcctcccgtc
     3421 cgggtaggtc acccactcct cgttatgcag cggccgctcg gcggctagcg cttctttatc
     3481 cttctcccgg tagaaatcgg cgatacccct gtcgtaaaaa tcgtaatccg tcttcccgac
     3541 cgtctccgac tcgcttctac ccgtcatctc ctcatgaggc cggttgcacc cgaggaagac
     3601 cccgtcggta tttttgtaaa aaatgatatc gggaatagag ttcaacatgg aggcgatcag
     3661 gcccgcctgt tttctgacct cgtcctccct ctcccgccgc tccgtggagt cgacgacgta
     3721 gccgtgcatc agcacgctcc cgtcgggcag ccgttcggga ttcccaatgg tgaagagcca
     3781 gcgctctcct ttttccggca acaccaccct gaactcgtgc tcccagacct ccatcgtcct
     3841 ggaggaagcg gcgagacgct cttcgagccc ttccctgtcc tccggatgca ccagcgcaaa
     3901 aaaggagccg atgtcccgga tcacctcgtc cggttcgagt ccgcatacat ccgtgaggtt
     3961 gtccgatacg aagagcagac cgattggtcc tccgggttct tggcgatact gatagagcac
     4021 ccccgggatc tgcctcgaca gcttcttcaa cagcgcgtcg ctctcccgga gcgccttttg
     4081 ctgcgcaagg attccggcat ccacccgcct cagcagcgtg aagagaagaa tgagcagcgc
     4141 cgccgcgatc gtcccgccga tgccccacat catcgcgacc tgcctccgaa gatggttcct
     4201 gtccgccgtg atatccgtca tgatcgcaaa ttctccgacg ctttctcccg agacg
//
LOCUS       DAOSWW010000099         4241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14282_length_4241_cov_2.169135, whole genome shotgun sequence.
ACCESSION   DAOSWW010000099 DAOSWW010000000
VERSION     DAOSWW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14282_length_4241_cov_2.169135"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1972)
                     /locus_tag="PK442_03100"
     CDS             complement(<1..1972)
                     /locus_tag="PK442_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOO86665.1"
                     /translation="MAVVEMTGISMVGPREEIERVAAGLLSLGNFEPVPLDFAMEKHP
                     GALSGSGGSRIATFRENPYDELLEVLCEIQALVSDEAAQGRTSGAIFPERGGGASARP
                     QISSGELYDRLLPSFASLPEACGLNRRHESGSWTMPPGGSGERSIVCPSLEEASARVK
                     GLQRRIKFWRERSEKLQEEREKLLAAKVFLDALASAGLDRTKSPPGAGGGKGRPDVAS
                     GPPDAAPGSTAWLERALDTRYLSLTFGRLSTDNYKRLAEMAGITPLLVFPVVTEKDYV
                     LVAIFCSRDYHPEAEKLFRVVYLKEYVLQEILEGSGGAMKEYLEEQLQRSERALRALR
                     EAPRQYLERHRKELELLYCAVYAKQRIYSLCRNRGELGDVFIVSGLVPSPTLPQVEDV
                     IERFGPNTLLLTEKDDALKRRGRRLPTLLKNTFPVRVFQEIVALYSLPSYDDIDPSPL
                     VALSFCIFFGFMFGDVGHGLLISAGAWFLARKGILKKAFSSVLQIAGFSAAAFGFLYG
                     SIFGSEELIPALWTSPMHGINDLILASLVTGTGFLSLGIALNILTLRRKGRIGKMLFD
                     CEGLAGLLFYWTAVAAGAFAFTSGEGVPKPLLFALAALFLIIMCSSLLERLFFGRGEE
                     DEGGVVHTFSVLHALLSFVSNTASFVRLAAFAL"
     gene            complement(1963..3093)
                     /locus_tag="PK442_03105"
     CDS             complement(1963..3093)
                     /locus_tag="PK442_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOO86666.1"
                     /translation="MSFLSAGERAAVTGKARVFRGRLLVPDDYLHLLKCGTVGEVIMY
                     LGRTPSYSSYFHGEYSEDIHRGEIEGILSIVPMLEELPFCRYLGPERASLLHAWGERF
                     DLEVVKRVLRIIATGMGDREALRRRVSSVPITLADAKKLLAAQTFRDVIEAIKGYPLE
                     NVLTEPLRQASETYSGGSVNLFRAKMAMDAFFMTRILSAGEKLPGSEGKGIRSIFGTR
                     ADLINLYWIYRGRRFFAMTPEEALAITLPVRYTLNFETLRSFAFAPDIPAMLRLIQEC
                     PYGAAFTTALGESTLEVSEIILEHNLYRILWRAATKIFASGTSGVHVVLAYLILRELE
                     VKDLFTIIEDIRYDYDKKRAREFLIHPGANAFTSGDRGMSWQ"
     gene            complement(3119..3454)
                     /locus_tag="PK442_03110"
     CDS             complement(3119..3454)
                     /locus_tag="PK442_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86667.1"
                     /translation="MNLSAVLGELLASEDEAVNGVETAKRKASKILREAREHFAADQE
                     ARLAGVRAQSKTIVESALHSAELEAAQIAEMGANGRRKMEEHFAESAPEIVRSLVMET
                     AARYGRLGG"
     gene            complement(3451..4083)
                     /locus_tag="PK442_03115"
     CDS             complement(3451..4083)
                     /locus_tag="PK442_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOO86668.1"
                     /translation="MTQKIAPTRGNMVKFASSLKLARKGRDLLEQKRTILLMELTGKI
                     REARDLQRDLELVFSDAYFSLQMAGLSVGIDNVEELAYSVPEVQDFTIRLHSVMGVEI
                     PKVDPMEKPPAPCYSFLGTSGALDAAYSRAQDVLALIARLAEVETSVYRLAVQIRKTF
                     RRVNALEKVVIPSNKRTLAWIANVLEENDREDFTRMKMARKNTSEGGGAR"
BASE COUNT          793 a         1443 c         1272 g          733 t
ORIGIN      
        1 tcagcgcgaa ggccgcgagt ctgacgaagg acgccgtgtt gctgacgaac gagagcagcg
       61 cgtgaaggac gctgaacgta tgcaccacgc ccccttcgtc ctcctcgccg cgcccgaaga
      121 agaggcgttc cagcagcgag ctgcacatga tgatcagaaa gagcgccgcc agcgcgaaga
      181 gcagcggctt cgggacgcct tcgccggagg tgaaggcaaa cgcccccgcg gcgaccgccg
      241 tccagtagaa gagcagtccc gccagtccct cgcagtcgaa gagcatcttc ccgatccgcc
      301 ccttgcggcg cagcgtcaga atgttgagcg cgatcccgag gctcaggaag cccgtccccg
      361 tgacgaggga ggcgagaatc aggtcgttga tgccgtgcat gggggatgtc cagagagcgg
      421 ggatgagctc ctcggagccg aagatgctcc cgtagagaaa gccgaatgcc gccgcggaaa
      481 aaccggcgat ttgcagaacc gacgagaacg cctttttcag aatgcccttc cgggcgagga
      541 accaggcgcc cgcggagatc aggaggccgt gtccgacgtc gccgaacatg aagccgaaaa
      601 agatgcagaa tgagagcgcc acaaggggcg aagggtcgat gtcgtcgtag gaggggaggc
      661 tgtagagagc cacgatctcc tgaaagaccc gtacaggaaa ggtgttcttc aaaagtgtgg
      721 ggagcctgcg cccccggcgc ttcagcgcgt cgtccttctc cgtcagcaga agggtgttcg
      781 gcccgaagcg ttcgatgacg tcctccacct gcggcagcgt cggggagggg acgaggcccg
      841 aaacgatgaa cacgtccccc agctcgccgc ggttccggca gagggagtag atccgctgct
      901 tcgcatacac ggcgcagtag agcagttcga gctcttttct gtggcgttcc agatattgcc
      961 gcggagcctc gcggagtgcg cggagcgcac gctccgaacg ctggagctgc tcctcgagat
     1021 actctttcat cgcgcctccg gagccttcga ggatctcctg caggacgtac tccttcaggt
     1081 agaccacgcg gaacagcttc tccgcctccg ggtggtagtc gcgcgagcag aagatggcca
     1141 ccagcacgta gtccttctcc gtaaccaccg gaaagaccag cagcggcgtg attcccgcca
     1201 tctccgccag acgtttgtag ttgtccgtgc tcaggcgtcc gaaggtcagg gagagatagc
     1261 gcgtgtcgag cgcccgctcc agccatgccg tcgagccggg cgcggcgtcc ggaggcccgg
     1321 aggcgacgtc gggacgtccc ttaccgcccc ctgcgcccgg agggctcttc gtcctgtcga
     1381 gccccgcgga agcgagcgcg tccagaaaga ccttcgcggc cagaagcttc tcacgctctt
     1441 cctgcagctt ttccgaacgc tcccgccaga atttgattct cctctggagc cccttgacgc
     1501 gggccgacgc ctcttcgaga gagggacaga cgatgctgcg ctcgccggaa ccgccgggcg
     1561 gcatcgtcca cgagccgctc tcgtgccgcc ggttcagtcc gcacgcttcc ggcagggagg
     1621 cgaaggacgg gagcagacgg tcgtaaagct cgcccgagga gatctgcgga cgcgcggacg
     1681 caccgccccc cctctcgggg aagatcgcgc cggaggtcct cccctgcgcc gcttcgtccg
     1741 acacaagcgc ctgaatctcg cagagcacct ccagaagctc gtcgtacggg ttctccctga
     1801 acgtcgcgat ccgcgacccg ccggagcccg acagggcccc cgggtgcttc tccatagcga
     1861 aatccagcgg gacgggctcg aagttcccga gggagagcag acccgccgca acgcgctcga
     1921 tctcctcccg cggaccgacc atcgaaattc ccgtcatctc cactactgcc atgacatccc
     1981 ccgatctccg gatgtgaagg cgttcgcccc ggggtggatc aggaattcgc gcgccctctt
     2041 cttgtcatag tcgtaccgga tgtcctcaat aatagtgaac agatccttca cctccagttc
     2101 ccgcagtatc agatacgcca gcaccacatg caccccggaa gttcccgagg cgaaaatctt
     2161 cgtcgccgcc ctccacagga tgcggtagag gttgtgctcc agaataatct cgctcacctc
     2221 gagcgtcgac tcgccgagcg ccgtcgtgaa cgccgctccg tacggacact cctgtatcag
     2281 acgcagcatc gccggaatgt ccggggcgaa ggcgaacgaa cgcagcgttt cgaaattcag
     2341 cgtgtaccgc accggcagcg tgatcgcaag ggcttcctcc ggcgtcatcg caaaaaaacg
     2401 tcgtccccgg tagatccagt agaggttgat caggtcggcc cgggtgccga agatgctccg
     2461 gatgcccttc ccctcgcttc cgggaagttt ttctcccgcc gacaggatcc gcgtcatgaa
     2521 gaaggcgtcc atcgccatct tcgcccggaa caggttgacc gatcctcctg agtacgtctc
     2581 ggacgcctgc cgcagaggct ccgtcagcac gttctccaaa gggtacccct tgatcgcctc
     2641 gatcacgtcg cggaaggtct gcgctgcgag cagctttttc gcgtccgcca gcgtaatcgg
     2701 tacggaggac acccggcggc gcagcgcctc cctgtcgccc atacccgtcg cgatgatgcg
     2761 cagcacgcgc ttcaccacct ccaggtcgaa gcgctctccc cacgcatgca aaagggaggc
     2821 ccgctcaggc ccgaggtacc ggcagaaagg cagctcttcc agcattggaa cgatcgagag
     2881 gattccttcg atttccccgc ggtgaatgtc ttcggagtac tctccatgga agtacgagga
     2941 gtaggagggg gtgcgcccca gatacatgat gacttcccca accgttccgc acttcaggag
     3001 atgcagatag tcgtcgggga cgagcaggcg tccccggaag acgcgcgcct ttcctgttac
     3061 cgcagcccgt tcgcccgccg aaagaaagga catgcgcacc cctcccccgc gccttcggtc
     3121 atcctcccag tctcccgtag cgcgcggcgg tctccatgac aaggctccgc acgatctccg
     3181 gggccgactc ggcaaaatgt tcctccatct tccgccgccc gttggctccc atctcggcga
     3241 tctgcgccgc ctcaagctcc gccgagtgca gggcggactc cacgatcgtc ttcgactgcg
     3301 cccggactcc ggccagcctc gcctcctggt ccgcggcgaa atgctcccgc gcctcgcgga
     3361 gaatcttgga cgccttccgc ttcgccgtct ccactccgtt gacggcctcg tcctccgagg
     3421 cgagaagctc gcccagcaca gcgctcagat tcatcgcgca cctccccctt ccgaagtatt
     3481 ctttcgcgcc atcttcatgc gcgtgaagtc ctcccggtcg ttctcctcaa gcacgttggc
     3541 gatccacgcc aaggtcctct tgttcgacgg aatgacgacc ttctccagcg cgttcaccct
     3601 ccggaacgtc ttgcggatct gcaccgccag ccggtagacc gacgtctcca cctccgccag
     3661 ccgcgcgatg agcgcaagca cgtcctgcgc ccggctgtag gcggcgtcga gcgctcccga
     3721 cgtcccaaga aaggagtagc agggagcggg cggcttctcc atcggatcca ccttcgggat
     3781 ctccaccccc atcacggagt gcaggcggat ggtgaagtcc tgcacctcgg ggacggagta
     3841 ggcaagctcc tccacgttgt cgatccccac ggagagcccg gccatctgca ggctgaaata
     3901 ggcgtcggaa aagacaagtt cgaggtcgcg ctgcagatcc cgcgcctcgc gtatcttccc
     3961 cgtcagctcc atcagcagga tcgtccgctt ctgctccagg aggtcgcgcc ctttccgggc
     4021 cagcttcagc gacgacgcga acttcaccat gttgccccgc gtgggggcga tcttctgggt
     4081 cacctgccgc acctcccgtt cacatgatga gcactttaaa actccgggga agaaaagtca
     4141 acagtgaagg gagggggctg tgacgagtag aagctccccg gttccgattg cgtggtatac
     4201 tttgtgcagt cgctgcaacg gaaaggggcg gccttccgcg c
//
LOCUS       DAOSWW010000100         4225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14364_length_4225_cov_4.589448, whole genome shotgun sequence.
ACCESSION   DAOSWW010000100 DAOSWW010000000
VERSION     DAOSWW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14364_length_4225_cov_4.589448"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PK442_03120"
     CDS             <1..805
                     /locus_tag="PK442_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86669.1"
                     /translation="STKLEALKERSLKSRFDRLGVELRKESLALRKREFACEEDARRG
                     GAELLEKAAKYGIVAGFSVELEEKAVRGKGRPKKDAPPPATNRTWRAVVEIGGVEEKT
                     WKSAMDRESTFILVYRMEKAVERKNPAEILRTYKNQNVVEQGFRFLKQPIYLGPVLLK
                     KPERVEALGYVFLLVLLLAKYLEYRVRASLEKEGDALRVGGQKLARPTTQTILYHFGT
                     VPLLESGGKRFMPNPPGGNGTRIMDALGFSWELYTHGDCEDRFMERFRS"
     gene            complement(920..1285)
                     /locus_tag="PK442_03125"
     CDS             complement(920..1285)
                     /locus_tag="PK442_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86670.1"
                     /translation="MNIIIQTVEGVLHRFIRAALSIVEYDAFVPGWTCENLIFCTLKK
                     TGGENDDEAELQTFFRTTTLAESCTRILDPVFRTSAKEHLDFPANNGRLQGKAAFPAP
                     WQPNIVANNAPEDSNQPTR"
     gene            complement(1508..1825)
                     /locus_tag="PK442_03130"
     CDS             complement(1508..1825)
                     /locus_tag="PK442_03130"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO86671.1"
                     /translation="MNERYGLPEETIAMIAAVFTRHPEVERVILYGSRAKGNYRSGSD
                     IDLALQGGDGMTERVLYSIVRELDDLPVPYSFDVAIHSMIANEALLEHIRRRGTVVYE
                     RRR"
     gene            complement(1822..2241)
                     /locus_tag="PK442_03135"
     CDS             complement(1822..2241)
                     /locus_tag="PK442_03135"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOO86672.1"
                     /translation="MEDIRWIQRFDSFLRALSQLEEAYALAASRRLSRLEEQGLIQAF
                     EFTHELAWKTLKDFLESRGTQDLYGSKDTTREAFRNGLVNDGDVWMDMIVSRNLTSHT
                     YDEATASRISARILENYFGEFKRFRAAMERFKETAPS"
     gene            2432..2653
                     /locus_tag="PK442_03140"
     CDS             2432..2653
                     /locus_tag="PK442_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86673.1"
                     /translation="MPMTVEREKLAQNILALTDEEVSAVAAFVRNLREDEPPLSDDEL
                     AQMAESDEDIAAGRLTPWKEARRRLEALP"
     gene            complement(2909..3322)
                     /locus_tag="PK442_03145"
     CDS             complement(2909..3322)
                     /locus_tag="PK442_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOO86674.1"
                     /translation="MKSERTTAIYPAIFSHAEDGITITFPDLPGCISCAQTDEEALHM
                     ARDALGAWIVATEDLGEPVPLPSPTSKLRPKKNEAVCLVDVWLPLFREEKRSGSVKKN
                     VTVPLWLNVLAEKAGLNFSQILQAGLKSTLGIQDR"
     gene            3788..>4225
                     /locus_tag="PK442_03150"
     CDS             3788..>4225
                     /locus_tag="PK442_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO86675.1"
                     /translation="MKKFAAVLAACAVLSISGAAFANALVMNGSTTVLPIAQAAAEKY
                     MAANSDVKISVSGGGSGNGIKAIIDGTTDIADSSRFIKTDEVKAAVEKGTYPVPFAVA
                     MDALLPIVHPENPVSDLSIEQLRDIYAGKITNWKDVGGDDKGIA"
BASE COUNT          895 a         1098 c         1239 g          993 t
ORIGIN      
        1 ctcgacgaag ctcgaagcct tgaaggagag gagtctgaaa agccgtttcg acagactggg
       61 cgtcgaactg cgcaaagaga gtctcgcgtt gcggaagcgg gagttcgcct gcgaggagga
      121 cgcccgaaga ggcggagcgg aacttctgga gaaggcggcg aagtatggga tcgtcgccgg
      181 attttccgtc gagctcgagg agaaagccgt acggggcaag ggcagaccga agaaggacgc
      241 tccccctccg gcgacgaacc gtacgtggag ggcggtcgtc gaaataggag gagtcgagga
      301 gaaaacgtgg aaatccgcca tggacaggga gtcgacattc attctcgtgt atcggatgga
      361 gaaggctgtc gagcggaaga atcccgcgga aatcctgagg acgtacaaga accagaacgt
      421 cgtggaacag gggttccgct tcctcaagca gccgatctac ctcggccccg ttctgctgaa
      481 gaagccggaa cgcgtggaag cgctgggata cgtctttctt ctcgtgctgc ttctcgcgaa
      541 gtatcttgaa taccgggtac gggcgtcgct ggagaaggaa ggcgacgcgc tgcgggtcgg
      601 aggacagaaa ctcgcccgtc ctacgacgca gacgatcctg taccatttcg gaaccgtgcc
      661 gctgctggag agcggaggga aacggttcat gccgaatccg cccggaggca atggaacccg
      721 catcatggac gcgctcggtt tttcctggga gctgtacacg cacggagact gcgaggatcg
      781 cttcatggag cgtttccgca gctgagagga gcatcccgtt gtaacgcccc gacgaggcga
      841 agctttctgt agctgcggat ggatggattc cgttatttca acgggatcgt gtgttgtatg
      901 gacttttaca gccctattct catcgagtcg gctggttcga atcctcgggg gcgttgttcg
      961 cgacgatatt cggttgccaa ggtgcaggaa aagccgcttt cccttgaagg cgtccattgt
     1021 tcgcaggaaa atcaagatgt tcttttgctg aggtgcgaaa tacgggatca agaatccgtg
     1081 tacaactctc cgcgagcgtt gtggttctga agaaggtctg caattcagct tcatcatcgt
     1141 tttcacctcc ggtttttttc agggtgcaga agatcagatt ttcgcaagtc cagcctggta
     1201 cgaatgcatc gtattcaaca atcgaaagag ctgctctgat gaagcggtgg agaactcctt
     1261 caactgtttg tattattata ttcatctttt cgctttgtca agacgctgat tccgtccgct
     1321 gttcgcagga aaccggtaca gacgttttta cgagaagaac gccggaaaat tacactgctc
     1381 gaagaccata gagctccagg gagttccgct tcaggttgcc gtctacgggt gggagttgca
     1441 accgtaggct tattgatggc attttttttc tcggcgatga ggatgtgggt gaggaaaacg
     1501 tttgccgtta ccggcgcctt tcatacacca ccgttcctct gcggcggata tgctcaagca
     1561 gggcctcgtt ggcgatcatt gagtggatcg cgacgtcgaa ggagtagggg accggaaggt
     1621 cgtcgagttc gcgcacgatg ctgtagagga cgcgctcggt catgccgtcg cctccctgga
     1681 gcgcaaggtc gatatccgaa ccgcttctat agttgccttt ggctcgcgat ccgtagagta
     1741 tgactcgctc gacttccgga tggcgggtga agacggcggc gatcatcgct atggtttctt
     1801 ccgggaggcc gtaccgttcg ttcatgacgg ggccgtttct ttgaagcgtt ccatcgcggc
     1861 gcggaagcgt ttgaattcgc cgaagtagtt ctccaggatt ctggcggaga ttctggaggc
     1921 cgtggcttcg tcgtaggtgt gcgaagtaag gttgcggctg acgatcatgt ccatccagac
     1981 gtcgccgtcg ttgacgaggc cgttccggaa ggcttcgcgc gtggtgtcct tcgagccgta
     2041 gaggtcctgg gttcccctgc tttcaaggaa gtccttcagc gtcttccatg ccagttcatg
     2101 ggtgaattcg aacgcttgaa taagcccctg ctcctcgagg cgggagagcc gacgcgacgc
     2161 ggcaagcgcg tatgcttctt caagttggga aagcgcgcga agaaagctgt cgaatcgctg
     2221 tatccaccgg atatcttcca tcggtgaagc ccctcctttt tttaaacaac tcccgacgct
     2281 cataatagca tacagccacc ggtctttctt gaacaggagt ccgggaagaa ggccgagccg
     2341 gtttcctgag aggcatcatg ctctaatgga cgctctctct gcggtgcgct atgaagcacg
     2401 ctataatgac gatgaaaggg gggtgacgac tatgccgatg accgtggaac gggaaaagct
     2461 ggcgcaaaac attctggcac ttacggacga ggaagtttct gcggtggctg cgtttgtgcg
     2521 caatctgcgt gaggacgagc cgccattgag cgacgacgaa ctggcacaga tggccgagtc
     2581 cgacgaagac atcgcggcgg gccgtctgac cccttggaaa gaggcaagac gacgtcttga
     2641 ggcgttgccg tgacgttggt aacacactct tttcgtgatc ttcttgaatc agcatggtga
     2701 tgaaaaataa aagcaaagcc tccatatttc cccgggtgtt ttttaaaaaa acagctactc
     2761 tctgctcctg cgccgttgat aagctctttc cagcgcctca tccgcaggta tgcaacgtgc
     2821 catgcccgta tccatctcac gttcccggcg ggaggcttct ttgatccaca tgtcttcaat
     2881 cttgtggtcc gtcattttcc acagctcctc aacggtcttg aattccgaga gtggatttaa
     2941 gccctgcctg gagaatttgg gagaagttca gcccggcttt ttcagccagc acgttcagcc
     3001 aaagcgggac cgtgacattt ttctttacgg agccggaccg cttttcttcg cggaagagcg
     3061 gcagccacac gtcgaccagg cagacggctt cgttcttttt cgggcgtagt ttcgaagtag
     3121 gagagggcag cgggactggt tctcccaagt cctcggtggc gacgatccat gctccgaggg
     3181 cgtctcgtgc catatgcagc gcctcctcgt ctgtttgagc gcaggaaatg catcccggga
     3241 ggtcggggaa ggttattgtg ataccgtctt ccgcatggga gaagatcgcc ggatagattg
     3301 cggttgtgcg ttcgcttttc attatttcgc ctccttcttt tttagcgtat ttctttgatc
     3361 cctgtttgac gtcgtatagc agcaagagta tctttgggaa tatccttgcc gtatggaatc
     3421 gtggttctct gcttcgtctc cggatgaaag aaaaccatgt ggcttccttt gcctcttcct
     3481 gtgctgatcc aaccggcttc cagtagaatt ttgactattt catccctacc gattaccagc
     3541 accccccctt tgaatgataa gtatatttta tactccttta gtgagacgtc aagtatgata
     3601 tatacgtact tgtattttat gcgtataaaa tgatgcatct tcccccccgt tacgaacgta
     3661 acgtcatcgt tactcatctg ttacatactt ttgtaacatc cgttacgctc gcctcctata
     3721 ctgtgcgtgt cgatcgggga tggtccccgg cgcagactaa gacaagaaat caaggaggct
     3781 attcggtatg aagaaattcg cggcagttct ggcggcatgt gcggtcctct ccatttccgg
     3841 cgcggcgttt gcgaacgccc ttgtgatgaa cggttccacc acggtgctcc ccatcgccca
     3901 ggcggcggcg gagaagtaca tggcggccaa cagcgatgtg aagatcagcg tttccggcgg
     3961 cggcagcggc aacggcatca aggcgatcat cgacggcacc acggacatcg ccgactcctc
     4021 ccgcttcatc aagacggacg aagtgaaggc ggcggtggag aagggaacgt acccggtacc
     4081 cttcgcggtg gccatggacg cgctgcttcc catcgtgcac ccggagaacc ccgtgagcga
     4141 cctgagcatc gagcagcttc gcgacatcta cgccggcaag atcaccaact ggaaagacgt
     4201 cggcggcgac gacaagggaa tcgcg
//
LOCUS       DAOSWW010000101         4223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14379_length_4223_cov_3.865643, whole genome shotgun sequence.
ACCESSION   DAOSWW010000101 DAOSWW010000000
VERSION     DAOSWW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14379_length_4223_cov_3.865643"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PK442_03155"
     CDS             <1..834
                     /locus_tag="PK442_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO86676.1"
                     /translation="MAKIEAMYEEANPGVDIQRESGGSAALARKIIDLGGACDAFFSA
                     DYMVIEKLLRPAAADWNILFASNAIVLMYGPKSKYGEEIGAKNWTEVMLRPDVRWGHS
                     EPDADPCGYRSLMVLQLAEKFYGDEALYERAMKHSGRGVRQKAIELVSMVESGAMDYA
                     FEYKSVAVQHGLKYLDLPAEINLMEPSRADDYAAATVELAGKEPGKKMLVKGEPIVYG
                     LSIPKDAPNADGAMKFVEFVLSPKGGLAVFQEMGQDIVGPKAMGAGDSIPAVLKALLK
                     N"
     regulatory      967..1087
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1142..1837
                     /locus_tag="PK442_03160"
     CDS             1142..1837
                     /locus_tag="PK442_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO86677.1"
                     /translation="MDYLSEGFLQGFSLIFSGNEEVWSAVAVTLKLSGLSMLVTLLFG
                     IPAGFALGYFTFPGKSLLRLLVDTLLSIPTVVVGLFVYAFISNRGPLGQFELLFTIPG
                     MAIGQTILALPIVLALTASAVESADSRLRFTLLTLGARGRRLLFSTLWEGRHQILLAA
                     VTAYGRVTAEVGVSMMLGGNIKWHTRTVTTAITLETGKGEFSTGIALGVLLLCLSFVL
                     NWAVSFLRRRGAA"
     gene            1866..2522
                     /locus_tag="PK442_03165"
     CDS             1866..2522
                     /locus_tag="PK442_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO86678.1"
                     /translation="MHKTPLYELTNIVCAYEDEPVLQVDSLAIPDEGPVAFVGHNGSG
                     KSTLLKLLSFLLPQVSGELRFLGETQDKKDDARLHATLLLQEPYLLRRSVFENIAYGL
                     RARGEASLRERVVEALEMVGLSADFARREWYQLSGGEARRVALASRLALRTRVLLLDE
                     PTANVDEENAQLMAEAVLDAWKRWNTTPLIATHDLQWLDTVTQRRIFMRRGKIERIAL
                     "
     gene            2734..3483
                     /gene="modA"
                     /locus_tag="PK442_03170"
     CDS             2734..3483
                     /gene="modA"
                     /locus_tag="PK442_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO86679.1"
                     /translation="MKLYRNRWVFSILAFFLVFGTALPSSAENAETVAAAASLQSLMA
                     KTIPWFNEQRGTGLQGVYGASGNLARQIESGAPFDLFLSADEKWARYVEEKGLLDGEP
                     LPFAAMPLVLWHSGETAPSLEILRSEKLTVAIANPETAPFGRRAKAYLMEKGLFEAIE
                     TSGRLIIGGDVLKTGLAAKSGGADLAILPLSTASSLGEGSWTLLDAEPQTLFGGVVKG
                     RSTPAIEAFWTFLRSPEAVPFLKEFGFLPPR"
     gene            complement(3539..3748)
                     /locus_tag="PK442_03175"
     CDS             complement(3539..3748)
                     /locus_tag="PK442_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86680.1"
                     /translation="MAPPHAPVKHDRPGMSFFAQMLIVLLVLALLLLLVLVADTYLHD
                     IGKDWSHSTLVKMILERREVVPLFE"
BASE COUNT          802 a         1303 c         1296 g          822 t
ORIGIN      
        1 atggcgaaga tcgaagcgat gtacgaagaa gccaaccccg gcgtcgacat ccagcgcgag
       61 tcgggcggca gcgcggcatt ggccagaaag atcatcgatc tcggcggagc gtgcgacgcg
      121 ttcttttcgg ccgactacat ggttatcgag aagctgttgc gcccggcggc ggcggactgg
      181 aacatcctct tcgccagcaa cgccattgtc ctgatgtacg ggccgaagtc gaagtacggc
      241 gaggagatcg gcgcgaagaa ctggacggaa gtcatgctgc gccccgatgt gcggtggggg
      301 cactccgagc ccgacgcgga tccctgcggc taccggagcc tgatggtgct ccagctcgct
      361 gagaaattct acggtgacga ggcgctctac gagcgggcga tgaagcactc cggccgcggc
      421 gtccgccaga aggcgatcga gctcgtctcc atggtggaga gcggcgcgat ggactacgcg
      481 ttcgagtaca agtccgtggc cgtccagcac ggactgaaat accttgatct gccagcggag
      541 atcaacctga tggagccttc cagagccgac gactacgcgg cggcgacggt cgaactcgca
      601 gggaaggagc cggggaagaa gatgctcgtc aagggcgaac cgatcgtcta cggcctctcg
      661 atcccgaagg acgcccccaa cgcggatggc gccatgaaat tcgtcgagtt cgttctctcg
      721 cccaagggcg gcctcgccgt cttccaggag atgggacagg acatcgtcgg cccgaaagcg
      781 atgggcgccg gcgattcgat ccctgccgta ctgaaagcgc tgctgaagaa ctgacaatca
      841 ttcgaagtgc gcagagggaa tgccccacgt tccccctgcg cacttcgccg aaacggctgc
      901 cgttcccctt cccccgacta attttcacat tctttcttgc ttccccgaca gagaaaagat
      961 acaatagcag ggaacttcct ccgagcctca ggaccttcag gcgtttcgcc cacggtcttc
     1021 cggccgaaag ggtgtttccg gaaacggaga cgcctcccgt gtggaaaggg gggacaaggc
     1081 cgccgcttcg acgtgccttc tccgcgcccg cgggggagac gcgacgaagg ggtgtatatt
     1141 tttggactat ctctccgaag gatttctgca agggttctcg ctcatcttct caggaaacga
     1201 ggaggtttgg tcggcggttg cggtgacgct gaaactctcc ggcctttcaa tgctggtgac
     1261 cttgctcttc ggcatcccgg cgggattcgc cctcggctac tttaccttcc ccggcaagag
     1321 tcttctccgt cttcttgtag acacgctcct ctcgataccg accgtggtgg tcggcctttt
     1381 cgtctatgcg ttcatctcga accgggggcc tttgggtcag tttgaactgc tcttcacaat
     1441 tcccggcatg gcgatcggcc agaccatact ggcgctcccc atcgtcctcg cgctcaccgc
     1501 ctccgcggtc gagagcgccg acagccgtct tcgattcacg ctgctcacgc tcggtgcacg
     1561 gggccgccgc cttctcttct ccacgctctg ggaggggcgg catcagatac tgctcgcggc
     1621 cgtcacggcc tacggcagag tgaccgccga ggtgggcgtc tcgatgatgc tcggcggcaa
     1681 catcaagtgg catacgcgga ccgtcaccac cgccatcacg ctggagacgg gcaagggaga
     1741 gttctccacg ggcatcgccc tcggcgtgct gctgctctgc ctctcgttcg tcctcaactg
     1801 ggccgtttcc ttcctgcgcc ggaggggcgc ggcgtgatcc ccgccgaaag gatgtgcgcg
     1861 ctgtgatgca caagacgccg ctctacgaac ttacaaacat cgtctgcgcc tatgaggacg
     1921 agcccgttct gcaggtggat tcgctcgcca tccccgacga agggcccgtt gcgttcgtcg
     1981 gacacaacgg aagcggcaaa agcacgctgc tgaagctgct ctccttcctt ctgccgcagg
     2041 tgtcgggcga gctgcgcttc ctgggggaga ctcaggacaa aaaagacgac gcccgtctgc
     2101 acgcgacgct tctccttcag gaaccctacc tcctccgccg ttccgtcttc gagaacatcg
     2161 cctacgggct gcgggcgcgg ggggaggcat cactgcggga gcgcgtggtc gaggcgcttg
     2221 agatggtggg gctctccgcc gacttcgcac ggagagagtg gtaccagctc tctggaggag
     2281 aggcccgccg cgtcgcgctc gcctcgcgcc tggcgctgcg gacgagagtg cttcttcttg
     2341 acgagccgac cgcgaacgtc gacgaggaga acgcgcagct catggcggag gccgtcctcg
     2401 acgcctggaa gcgctggaac accacgcctc tgatcgcgac gcacgacctc cagtggctgg
     2461 acacggtgac acagcgaagg atcttcatgc gccgggggaa gatcgagcgt atcgcgctgt
     2521 aaaacaaaag ggccttcgcg ctccagacaa ccgggagaaa ttccctgccc tcgaaaagtg
     2581 cggagagggg ctggagaaag agagaagtta gggggaggat tctttgaaat ccttcccttt
     2641 tttcggtcgc catacttttt cgcaaccgag ttgcattttt tgcgaacgaa gcctagaata
     2701 agcgcaactt tgttgcaaaa gggaggaaca ggtatgaaat tgtatcgaaa caggtgggtt
     2761 ttcagcattc tggctttctt tctcgtattc ggcacagcgc tcccctcgtc ggcggagaac
     2821 gcggagacgg ttgcggcggc ggcctcgctg caatccttga tggcaaaaac catcccctgg
     2881 ttcaacgaac agcgcgggac cggactccag ggggtgtacg gcgcttcggg caatctggcg
     2941 cggcagatcg agtcgggcgc gcccttcgat ctttttttaa gcgccgacga aaaatgggcc
     3001 cgttacgtcg aggagaaggg gctgctcgat ggagagccgc tccccttcgc ggcgatgccc
     3061 cttgtgctct ggcactccgg agagacggcg ccctctcttg agattctgcg ctcggaaaag
     3121 ctcaccgtgg ctatcgcgaa tccggaaacg gcgcccttcg gcaggcgggc aaaggcgtac
     3181 ctcatggaaa aaggactttt tgaagcgatc gaaacgagcg gcagactgat catcgggggc
     3241 gacgtgctca agacggggct ggcggcgaaa agcggcggcg cggatctcgc catcctgccg
     3301 ctgagcaccg cctcctcgct cggcgaagga tcgtggacgc tgctcgacgc ggagccgcag
     3361 acgctcttcg gcggggtcgt caagggacga agcactccgg cgatcgaggc gttctggacg
     3421 ttccttcgct cgcctgaagc cgtgcctttc ctgaaggagt tcggcttcct cccgcctcgt
     3481 tgaacactcg gaatcgaaaa gtaaactcac gacttcagtt cacgcaggag cggagagact
     3541 actcgaaaag agggacgacc tccctccgct ccaaaatcat cttcacgagc gtcgagtgcg
     3601 accagtcttt cccgatgtcg tgcagatacg tgtcggcgac gaggacgagc aagaggagca
     3661 gcgcgaggac aagcagaacg atcagcatct gagcgaaaaa ggacattccc gggcggtcgt
     3721 gcttcaccgg agcgtgtggc ggcgccattg cgatcacatc cttgacggca ttcttgaaaa
     3781 atagtcttcc ggagcataca ctccggtacg gcgaaagaac acccgtgaac atgccggaag
     3841 gtcttccgca accactattc tacaacatgc cgcgcgcaat cggcggaacg gcgcgctatg
     3901 ccgtaccgcc tccgtccgtt cttcgatctg aaacgatttt gatgcaacgc cgttttcctg
     3961 ataccatgag cacattctgc attcctggag gtctgccgca tgttcgatga acttttcctc
     4021 tcctctctct tccggatatc ggggctcgcc ccgctctatc ccctctgttc cgggcgctcc
     4081 acggctcgcg cgagcagagc gtgacggcga tgctccctcc gctcctcctc agcctccgcg
     4141 tcctgctgtt cgccgttccc ctgcttgttc tccttggagg agggatcggc tggctgcttg
     4201 cgcgggctaa tttccccggg aag
//
LOCUS       DAOSWW010000102         4220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14397_length_4220_cov_3.627611, whole genome shotgun sequence.
ACCESSION   DAOSWW010000102 DAOSWW010000000
VERSION     DAOSWW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14397_length_4220_cov_3.627611"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..761)
                     /locus_tag="PK442_03180"
     CDS             complement(108..761)
                     /locus_tag="PK442_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86681.1"
                     /translation="MHAALGIVRVLLCSNGSGDDDCRVCSAWSGERHPDLLYCGAPSK
                     PPSIDECREIIDAMAYRPVVSSKRVAVVFSADRMMLPAANSLLKLAEEPPEYVHMLFL
                     LTDEKLFLPTLRSRSWSVSLPVPRSGESMPPPQTEEEWIRWIEKNAKSEVDELIVLFA
                     PWISYEVERGAYDKAAALERIRLLIQTKRLSRTMVLDLIVLALKEGIVFEHSFGDLW"
     gene            complement(893..1327)
                     /locus_tag="PK442_03185"
     CDS             complement(893..1327)
                     /locus_tag="PK442_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HOO86682.1"
                     /translation="MRVKKTSKETLSGGEFSKTSKRSGQLPSRESTASVFSASLEDAE
                     IRDLLDRLDIIGRKLSIFPAEALLIQYRKLVSELVRRAVAGLRVRRDMKWRRGDKNFF
                     VIVEKTDSMLDELESVFAREGERTRMLQLMEEIKGCLFSLLF"
     gene            complement(1324..3798)
                     /gene="tmk"
                     /locus_tag="PK442_03190"
     CDS             complement(1324..3798)
                     /gene="tmk"
                     /locus_tag="PK442_03190"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOO86683.1"
                     /translation="MNLFRLLICALVLLLAPAEQSEASRFTPSVPVLGVENLRPGMKG
                     YALTVVSGREIVRFPVEIVSVIPGKGSPKHLIMIKASGSVIAATGGIAAGMSGSPVFI
                     NDKLIGAIGYGWNFSEHTLGLVTSFSDMASIWSWPEKIVSIPPATALDDADKKKDQED
                     ESQAGQEADNGKEDKEEDKLVVPVAAEQLSSPLFINGISTRKALETAALLGRDNFIPG
                     GGFTDAVPVEMNATMLPGDAITVLLAWGDVTIGATGTLTAVAPDGRFIAFAHPFLGRG
                     AVNYPVARAYIHKVIPSLESPFKVGSPLQIVGTVTQDRPQGIGGRIGYFTPSISATLD
                     FTDAERNTKERKRFHVVPDPFIGAKLTAGVFTGLIDDLWGRKGQGTATLTLNVQGRGL
                     ISGWTRTNMFFSDSDLAAAALKETSELLDVVFLNPFAEVYPLGITVTTEFSPMPKLMY
                     IEGVNLKGESFSSGETVEVEVLIRPYRKKQVKKSFTITIPKDASGICEILVRGGGIEP
                     LNQSAIVQGWKTIENFRQLFTEMSALETNNELIIEFNYEKRSKDGKGRSDEDEPVSKE
                     EQELLSELKQRRLKDGTLQIFKSEFVVEGLLRKLSLSRPATHRNQTSRKRRVNQMFIT
                     FEGIDGCGKTTQAALLANFLEGKYRGNRRVVWTREPGGWDGGSWIRERILEGDFSHPM
                     SELFLFLVDRCEHVRKEILPVLEGGGVVICERYTDSTLAYQSWGRGIPSEKVETLFEW
                     CSFPRPHKTFWLDVPVKTAMERVCLRGGRDRFEAEGYSFLERVREGFSSLAKREPQRI
                     CTVEASSEPQALASRILSLVEVSLSR"
     gene            complement(3827..>4220)
                     /locus_tag="PK442_03195"
     CDS             complement(3827..>4220)
                     /locus_tag="PK442_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOO86684.1"
                     /translation="LKVDTFRASGAGGQHVNRTDSAVRITHIPTGIVVGCQNERSQHM
                     NRQVAMQVLRSRLFEKATQERQQELLSIQGEKKEISWGSQIRSYVLHPYTIVKDHRTG
                     FEVGNVHSVLDGDIDGFIMSFLRWKRQK"
BASE COUNT         1039 a         1157 c          972 g         1052 t
ORIGIN      
        1 gaccgccaag aagagccagt tcagaacctc tcatcgtttc gacaacgagc caggttccct
       61 tggaagggaa aggggcttct atgttgagaa gcccaagata tcgaggttta ccaaagatcg
      121 ccaaatgaat gctcaaaaac aataccctcc ttgagagcca atacaataag gtcgagaacc
      181 atggttcggg aaagacgttt cgtctggatc aaaaggcgta ttcgctctag cgctgccgcc
      241 ttgtcatacg ctccccgttc tacttcatag gatatccatg gagcaaagag cacaatcagc
      301 tcgtcgactt cagacttggc atttttttca atccaccgaa tccactcttc ctctgtttgc
      361 ggcggcggca tgctctcacc cgaacgaggc acaggaagag aaacactcca cgaacggctt
      421 ctcagtgtgg gcagaaagag tttctcgtcc gtaagtaaaa aaagcatatg aacatactcc
      481 ggcggttctt ctgccagttt taacagactg tttgccgcag gaagcatcat tctgtctgca
      541 gaaaaaacaa cagcaactct ttttgatgat accaccggac gatacgccat tgcatcgatt
      601 atctcacgac actcgtcaat agaaggaggt tttgagggag cgccgcaata caaaagatcc
      661 ggatgcctct cgccggacca ggctgaacag accctgcaat catcatctcc cgaaccgtta
      721 ctacagagca gcacgcgaac aattccgagc gctgcgtgca tatggaggtt ctcgtcgatc
      781 actactgcgc acgagtgagg gaaggctcct cttgatgcca aaaacagcaa acgtttccat
      841 gccgaagagt ccttccatga agcgccgcgc tgctcggaga ttccttccgt gttcagaaca
      901 gaagagaaaa cagacatccc ttgatctcct ccattaattg caacatcctg gttcgctctc
      961 cttcccgagc gaaaacagat tccaattcat caagcatgga gtctgtcttt tccacaataa
     1021 cgaagaaatt cttatcccct ctacgccatt tcatatcccg ccgtacacgt aaaccggcta
     1081 ctgcccgtcg caccagctcg gaaacaagtt tacggtactg aataaggagc gcctccgcag
     1141 ggaaaataga aagttttctt ccgataatgt caaggcgatc caaaagatcg cggatttcag
     1201 catcctcaag agaagccgaa aaaacggaag cagtcgattc acgagaaggc aactgcccgc
     1261 ttcttttcga tgttttggaa aattctcctc cgcttagtgt ttctttggaa gtttttttta
     1321 ccctcaacgg gacaaagaca cctccacaag agagagaatt cttgacgcaa gagcttgcgg
     1381 ttctgaagaa gcctcgaccg tgcaaatcct ttgaggctct ctcttggcca gggaagaaaa
     1441 gccctcgcga accctctcaa gaaacgaata cccttccgcc tcgaaacggt cgcgtcctcc
     1501 gcggaggcaa actctctcca tcgcagtttt tacagggaca tcaagccaga atgtcttatg
     1561 cggcctcggg aaagaacacc attcgaacag cgtctctacc ttttccgacg gaattcctct
     1621 tccccaggat tggtatgcca gtgtagagtc tgtgtaccgt tcgcaaataa cgacgccgcc
     1681 gccctcaagt acgggcagaa tctccttgcg aacgtgctcg caacggtcca caagaaagag
     1741 aaacaactcg ctcattggat gagaaaaatc tccttccaaa atgcgttccc gtatccacga
     1801 gccgccgtcc caccctccgg gttctcttgt ccatactacg cgccggtttc cccggtattt
     1861 tccctcaagg aaattggcaa ggagtgcagc ttgcgttgtt tttccgcatc cgtcaatacc
     1921 ttcgaatgtg ataaacatct gattaactct ccgtttccgg ctcgtctgat tgcgatgagt
     1981 tgccgggcgt gacagtgata gttttcgaag aagcccctcg acgacaaatt ccgatttgaa
     2041 tatctgaagc gtcccatctt ttaaacggcg ctgtttcagc tcgctcagaa gctcttgttc
     2101 ttcttttgaa acaggctcgt cttcatcgga acggcctttt ccgtcttttg accgtttctc
     2161 gtaattaaat tcgatgataa gttcattgtt cgtctcaagt gcggacatct cggtgaaaag
     2221 ctgacggaaa ttttcaatgg ttttccagcc ttggactatt gccgattggt tcagcggttc
     2281 tatcccgccg cctcgaacaa gaatttcaca gataccggaa gcatccttcg ggatggtaat
     2341 cgtaaatgat ttctttacct gtttcttcct gtaagggcga ataagaactt caacttcgac
     2401 agtttctccg gaggagaacg attctccttt gagattcacg ccttctatat acataagctt
     2461 cggcatcggg gagaattcgg tagttacagt tatccccagg ggatacactt cggcaaaagg
     2521 gttcaagaac acaacgtcga gcagctcgct tgtctctttt aaagcggccg ctgcaagatc
     2581 gctgtctgaa aagaacatat ttgtcctcgt ccatccgctt atcaaccctc ttccctgcac
     2641 attaagggtg agggtcgccg taccttgtcc ctttcgtccc cacagatcgt cgatcagccc
     2701 ggtaaagact cccgcagtca gctttgcgcc tatgaaagga tccggaacga cgtgaaaacg
     2761 cttcctttct tttgtattcc gttcagcatc cgtaaaatcg agagttgcag aaatggaagg
     2821 cgtaaaatat ccgattcttc cccctattcc ctgcggtctg tcctgagtaa ccgtcccgac
     2881 aatttgcagc gggctcccta ctttaaacgg agactctaaa ctggggatta ctttatggat
     2941 gtatgccctt gccacaggat agtttacagc ccctctcccg aggaaagggt gagcgaaggc
     3001 gataaacctt ccgtcaggcg ccacagctgt taatgtgccg gtagcaccga tggttacatc
     3061 tccccatgcc agaagcactg taatggcgtc cccgggcagc attgtggcgt tcatctcgac
     3121 cggaacagca tcggtgaagc caccgcccgg aataaaattg tcccttccga gaagggcggc
     3181 agtttcaagg gccttgcgag tgctgatacc attgataaag agaggcgagg agagctgctc
     3241 tgcggctacc ggtactacaa gtttatcctc ttctttgtct tcctttccgt tatccgcttc
     3301 ttgacctgcc tgactctcgt cttcctgatc ttttttctta tctgcatcat caagtgccgt
     3361 ggctggagga atggacacga ttttttccgg ccaactccag atgcttgcca tatcgctgaa
     3421 agaggtgacc aatccgagcg tgtgttcgct gaaattccag ccatatccaa tcgccccgat
     3481 cagcttgtcg ttgataaata cgggactgcc gctcatgcct gcggcaattc cgcctgtcgc
     3541 cgcgattaca gagccggaag ccttaatcat tatcaggtgt ttagggcttc cttttcccgg
     3601 gatcacgctt acaatttcaa cagggaagcg cacgatctct ctgccggaaa cgacggtaag
     3661 cgcgtacccc ttcattccgg ggcgaagatt ttctaccccc agcaccggga cggagggagt
     3721 aaaccgcgac gcttcggact gctccgcagg agcgagaaga agaacaagcg cgcatatcag
     3781 taggcgaaat aagttcatgg gcggcgatcc tcccgagtaa attttattac ttttgtcgct
     3841 tccatctgag gaagctcata atgaacccgt caatgtctcc atcaagcacg gagtgaacat
     3901 ttccgacctc aaagccggtc cgatgatcct taacaatagt ataaggatgc agaacatagg
     3961 aacgtatctg gctcccccag cttatctcct tcttctctcc ctgaatcgag agaagttctt
     4021 gctggcgctc ctgagtcgcc ttttcaaaga gacggctccg aaggacctgc atcgcaacct
     4081 gacgattcat gtgctgggat ctttcgttct gacagccgac gacgatcccg gtgggaatat
     4141 gtgtaatacg gactgctgaa tcagttctgt tgacatgctg tccgcccgca ccgcttgccc
     4201 ggaatgtgtc gactttgagg
//
LOCUS       DAOSWW010000103         4206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14472_length_4206_cov_3.471934, whole genome shotgun sequence.
ACCESSION   DAOSWW010000103 DAOSWW010000000
VERSION     DAOSWW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14472_length_4206_cov_3.471934"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PK442_03200"
     CDS             <1..460
                     /locus_tag="PK442_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86685.1"
                     /translation="LRHTVFGRNYLATGGNARASFVAGIDTSKMVITGFVIAGLLSAV
                     AGMLTAGRQDSVSNTMGNGMTLLAFAGALLGGTSMSGGKGSAFGMLGGAILLGMFSNA
                     LNLLGVKVTLIHAAQGALIFVAILTDRFRISLRARLLRNEQLQKLKVAKG"
     gene            528..1586
                     /locus_tag="PK442_03205"
     CDS             528..1586
                     /locus_tag="PK442_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO86686.1"
                     /translation="MNCSFKKAITALLALFVFSAASCASEKQISIGMIIKEPSAPYIQ
                     AFIKAAEETAASLGVNIMIRDGEADSIKIMEIIDTFMIQRIDAFILGGAVDLRTLVPG
                     IVRLNEAGIPVAALDASPEGGVVDFFLSFDLAQSSARAAELFVEGIKSRNSGDIPEGV
                     VLEILGDKADMFSHACTEGFNSVLSKYPQLEIAQGEGKWNNTDSHAVTSDLLTRFGKR
                     VVGIYVQTPDIMGPGVVAAIEAAGLDSADYGICGICIGPEGIDLIKKGKMLGAVAQPA
                     YDSAALAVRFLVDKLRGNPVPRIGDTLTEDGALWSPAKVIKNPWAEDGAFIVLQGPLV
                     PSEVSPDDPRLWENMLTK"
     gene            1644..2438
                     /locus_tag="PK442_03210"
     CDS             1644..2438
                     /locus_tag="PK442_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO86687.1"
                     /translation="MYGKKRVSSVFCVLLVLFLFSIAGQAAAGARLDRVMETKLLRVG
                     TPGDYRPFAMLDKSTGKYEGHDIELAELLASELGVTVEFVPTTWSKLMEDYLAGKFDV
                     AVGGITRSLARMLKGDFLPPYAPNGKVAIIRKADKEKFTSLEAMDVPETTVIVNPGGT
                     NEKYVTANFKSAKVVVHQSNAEIPAMIAEGKGDVMISEVYEAVVYSRKDDRLYGAFTD
                     KPLTKISFMGFFIQQDDPDFLKIMHYLWNDAKLRGDLDALFAKWLK"
     gene            2515..2614
                     /gene="ffs"
                     /locus_tag="PK442_03215"
     ncRNA           2515..2614
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK442_03215"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2634..2972)
                     /locus_tag="PK442_03220"
     CDS             complement(2634..2972)
                     /locus_tag="PK442_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOO86688.1"
                     /translation="MAKDSLIEKLEKLPKDRKAMLNRLRRVEGQLRGIQRMIIEEKPC
                     YDILLQLSAARKAMQKACIEILKNYLHRCVHEAKAPDFDNLEKLIEALIEISPAKPAF
                     PENEEESDEE"
     gene            3185..3988
                     /gene="rpsB"
                     /locus_tag="PK442_03225"
     CDS             3185..3988
                     /gene="rpsB"
                     /locus_tag="PK442_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOO86689.1"
                     /translation="MAVVSMKQLLECGVHFGHQTRRWNPKMKPFIFTERNGVYIIDLQ
                     KTVRGLEKAYDYIREVARNNGTVLFVGTKRQAQDTIKDEAERCGQFFINQRWLGGLLT
                     NFPTIRKRVARMVELRKMEDEGNWSNLPKKEVIILRKELGKLEKYLKGITNMKAIPDA
                     IFLIDPRREENAVLEARKLGVPVIAIVDTNCDPEMIDFPIPGNDDAIRAIKLIAGLMA
                     NAFIEGRQGEDSEAGVLVQGEEGDDTADDGIIEKKERLHEVYDDSVEEA"
BASE COUNT         1012 a         1014 c         1180 g         1000 t
ORIGIN      
        1 gctacgccat accgtcttcg gcagaaacta tctggcaact gggggaaatg cacgtgccag
       61 cttcgtcgcg ggtatcgata cgagcaaaat ggtgataacg ggttttgtca ttgccggcct
      121 tctctccgct gttgccggta tgctgacagc cgggaggcaa gattcggtgt ccaacaccat
      181 ggggaacgga atgacgctcc ttgcattcgc cggagcgctt ctcggaggca cctccatgag
      241 cggagggaaa ggctccgctt tcgggatgct cggcggtgcg atccttttgg gcatgttttc
      301 gaatgctctt aatcttctcg gtgtcaaggt gacgcttata cacgccgctc agggggcgtt
      361 gatatttgtc gccatactta cagaccggtt tcgaatctcc ctccgggcac gattgcttcg
      421 taacgaacag ctccaaaaac tcaaagtcgc gaaaggatga tggcaataat tgagaatgag
      481 gaaagatttt aaagtacgtt catccactac atcgaggagg aatctgcgtg aactgctctt
      541 ttaaaaaagc aataacggcg cttctggcgc tcttcgtttt ttctgcggct tcatgtgcgt
      601 ccgaaaagca aatctccatc ggtatgatca tcaaagaacc ctcggcgccg tacattcagg
      661 catttataaa ggcagcggaa gagacagccg cctcgctggg cgtgaatata atgattcgcg
      721 acggcgaagc cgattctata aaaattatgg aaatcatcga tacttttatg atacagagaa
      781 tcgacgcgtt catccttggc ggcgctgtgg atttgcgcac gctggtgcct ggtattgtgc
      841 gcctcaacga ggctgggatc cccgtggccg cactggacgc atctcctgaa ggaggcgttg
      901 tcgatttctt cctctccttt gacctcgctc agtcgagcgc ccgcgccgcg gagcttttcg
      961 tcgaggggat caagtccaga aacagcggcg atatacccga gggcgtcgtt cttgagatac
     1021 tcggcgacaa agcggatatg ttcagccatg cctgcaccga gggattcaac tccgtgctgt
     1081 cgaagtatcc gcagcttgaa attgctcaag gagagggaaa atggaataat accgactccc
     1141 acgcagtgac ttccgatttg ttgacgcgtt tcgggaaacg ggttgtgggc atttatgtcc
     1201 aaacccctga tataatggga cccggtgtcg ttgcggctat cgaggccgca gggttggatt
     1261 ccgccgacta cggtatctgc ggaatatgca tcggccccga gggaatcgac ctcattaaaa
     1321 aagggaagat gctcggcgct gtcgcacagc ctgcctacga ctcggccgcg ctggcggtga
     1381 ggttccttgt ggacaagctg agagggaatc ctgttccacg gataggagat acgctgacgg
     1441 aagacggcgc tctctggtct cccgcaaaag tcataaagaa cccttgggcc gaagacggag
     1501 cctttatcgt tctccaagga ccgcttgttc cttcagaagt ctcccctgac gacccccgtc
     1561 tgtgggagaa tatgctgacg aagtaaacgg gttttgagat gagagagaat gttacgaaac
     1621 aaaaaacgaa cggaggggta ttgatgtacg gaaaaaagag agtttcgagt gtattttgtg
     1681 tactgctggt gctgtttctt ttctcgattg cgggacaggc tgcagcagga gcgaggcttg
     1741 accgcgtcat ggaaacaaag ctattgcggg tggggactcc cggggactat cgcccattcg
     1801 ccatgctcga caagagtacg ggcaaatacg aggggcacga tatcgaactt gcggaacttc
     1861 ttgcgtcgga gcttggcgtg accgttgaat ttgttcccac cacctggtcg aagcttatgg
     1921 aggattacct tgcaggcaaa ttcgacgtcg ccgtcggagg tataacccgg agccttgccc
     1981 gtatgttgaa gggggacttt ttgcctccgt atgcaccaaa cggcaaggtt gccatcattc
     2041 ggaaggctga caaggagaag ttcacttcgc ttgaagcgat ggacgttccc gagacgacag
     2101 tcattgtgaa cccaggcgga acaaatgaaa aatatgtaac cgcaaacttt aagagtgcga
     2161 aagtcgtagt gcatcagagc aatgccgaga tccccgcaat gatcgcagag gggaaaggcg
     2221 acgtgatgat cagcgaagtg tacgaagctg tcgtgtactc gcggaaggat gaccggctct
     2281 acggcgcctt taccgacaaa ccgctgacga agatcagctt catgggattt ttcattcagc
     2341 aggacgaccc cgacttcctg aagatcatgc actatctctg gaacgacgcg aagctgcggg
     2401 gagatctgga cgctcttttc gcaaagtggt taaagtagtc gctggtaaag tgctcgaagc
     2461 catgctataa tccactgcgc tgaaaacgtg cagaatgaac ggctgcctgc ggggaatcca
     2521 gtcccgtgcg gcggagacct tcgaaccctg tcaggcccgg aagggagcag cagtaaggag
     2581 aattctccgg gcgccacggg gaaactgggt ttcccgcagg cagccgttct gtattactct
     2641 tcgtcagact cttcctcgtt ctccgggaac gcaggcttcg ccggagatat ttcaatgaga
     2701 gcctcaatga gtttctcgag attgtcgaaa tcaggcgctt tcgcttcgtg gacgcatctg
     2761 tggaggtagt tttttagaat ttcaatgcat gctttctgca ttgcctttcg cgctgccgag
     2821 agttgaagga ggatatcata acaaggtttt tcttcaatga tcatcctctg aatgccgcga
     2881 agctggccct ctacgcggcg aagacggttc aacatcgctt tcctgtcctt gggtagtttt
     2941 tcgagttttt cgataagtga atccttggcc attactatgc ccccattcgg ttttttcagt
     3001 agagtaaata ctgggtatat ggtacttcga gacaggttga aggtcaacct gtctttgtgg
     3061 tatactctcc tggtcctcat cacacacaca aactgatggg gtggagggtg ccgtttcaaa
     3121 aaacggtttc cactttgttc gatgtttgtg gagggaaaac caacatattt caggaggggt
     3181 tattttggca gttgtcagca tgaaacagct tttggagtgc ggagtccatt tcggacatca
     3241 gactcgtcgg tggaatccga agatgaagcc tttcatcttc acggagcgca acggagtgta
     3301 tatcattgac cttcagaaga ctgtccgcgg ccttgaaaaa gcatacgatt acatccgcga
     3361 ggttgccagg aacaacggga cggttctttt tgtcggaaca aagcgccagg ctcaggatac
     3421 catcaaggac gaggctgaac gttgcgggca gttctttatc aaccagcgct ggctcggcgg
     3481 tcttctcacc aactttccga cgattcgcaa gcgcgttgcc cgtatggtgg aacttcgcaa
     3541 aatggaagac gagggcaact ggtccaatct ccccaagaaa gaagtcatta ttcttcgcaa
     3601 agagttggga aaactggaga agtatcttaa gggcatcaca aacatgaaag ccatcccgga
     3661 tgccatcttc cttatcgacc ctagaagaga ggaaaatgcc gtcctcgaag cgaggaaact
     3721 gggagttccc gtgattgcaa tagttgacac gaactgcgat ccggagatga tcgacttccc
     3781 gatccccggc aacgacgacg ccatccgcgc gatcaagctt attgccggac ttatggccaa
     3841 cgctttcatt gaaggacgcc agggagagga ttccgaagcc ggcgtgcttg ttcagggcga
     3901 agagggcgac gatacagccg acgacggtat tatagagaaa aaggaacgct tgcacgaagt
     3961 ctacgacgat tctgtcgaag aggcgtaagt tatagagacg aagccgaccc gaccgggaaa
     4021 tcctgttggg tcggttcttc gtgtgccggt ttttcagcca tctgtcagaa gaatacgttc
     4081 ctcaatgtgt ttctgaattg gattttttcc tcgttgagcg gggaagtttg tttaccggaa
     4141 agggaggctt ttttattatg gaaattagcg ccagcgctgt aaaggaactt cgtgaaagaa
     4201 caggcg
//
LOCUS       DAOSWW010000104         4198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14513_length_4198_cov_4.508327, whole genome shotgun sequence.
ACCESSION   DAOSWW010000104 DAOSWW010000000
VERSION     DAOSWW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14513_length_4198_cov_4.508327"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1472)
                     /locus_tag="PK442_03230"
     CDS             complement(192..1472)
                     /locus_tag="PK442_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO86690.1"
                     /translation="MIALMTITFVVCFVMGIPLALVMGITGFSVILAMGVPLQLVAQR
                     MFTGIDSFPLMAVPFFILAGDLMNRGGTTVRIIGFANSLVGHIRGGLAHACIVANMIF
                     AGISGSAVADASAIGSIMIPSMEKNGYDLDFSAALNSAAATIGPIIPPSIIMVIYGVS
                     VNVSVGGLFAAGFIPGIMLGLAMMIVVSRVSRKKNYAMSEGFSGARVRKELKSSVWAL
                     MAPVIILGGILGGVFTPTEAAAVAVIYSFFVGKFVFREITWKDLPGILFQSGITTGAI
                     LLIISLANVFAWVIAANQIPVKLSSMFLSATSNPYVFLLIVNILLLIVGMFMETGAAI
                     ILLAPILAPIATQLGVHPLHFGFMMVLNLAIGMATPPVGVCLFVSCGITGLSLEKVSA
                     AVMRFVLAEIAILALVTYVAPISLFLPRLLGFIR"
     gene            complement(1469..1999)
                     /locus_tag="PK442_03235"
     CDS             complement(1469..1999)
                     /locus_tag="PK442_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO86691.1"
                     /translation="MKFLEGVLACFDKAAFAAVALLMGSMGVVAFAAVFYRFVLHDPI
                     TWSEEAARYMMVWVTFLGAGYAMGKGKHIGVTMFVEKLPEGVRRKVIFVAEAVIMLFL
                     AAVTVQGVKLIVSLWVQTSPAMDLPMWLPYLAIPVGAVYMFLHLLRLMLSRSAQVLAT
                     ADIELDAALKRKGDDR"
     gene            complement(2101..3132)
                     /locus_tag="PK442_03240"
     CDS             complement(2101..3132)
                     /locus_tag="PK442_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOO86692.1"
                     /translation="MKKMFGLMCVVALTGLLAGSALAADFTLKLANAGPADPEDRTVI
                     AVEIFKNHVETKTGGSVKVEVFHASQLGNEKEILEGLALGTIELGTITTGPVPTLFKP
                     IMVFDIPFLFPNQVVAWEVLDGPFGRKLMEEMRKSTGIRTLAISENGYRHFVTGSKQV
                     KSMADMKGLKIRTMENPAHMKMVEALGGAPTPIAFGELYMALQQGVVDGCELPVTLIN
                     NGKYYEVQKHVILNGHLYNPLIMFVNDKVWEKLSQDQKAALYEGAQLFNIAQRAISER
                     QVQSGLLNVQKNGMTVYVPTAAELNEFRSAAQPAVLDYVKAQAGEQWVNEALEAVAAA
                     EAKMAEMLK"
     gene            complement(3217..3825)
                     /locus_tag="PK442_03245"
     CDS             complement(3217..3825)
                     /locus_tag="PK442_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86693.1"
                     /translation="MNASLMPELLERIQKAFRPIEVRPGAPLPKLPGALPYVKELSGY
                     PRFCGSTMGAEILWNMEILEKAASKPNDPDFTAEYTVAGRHTRVLGRNHRYLDYPVNV
                     FLNICMLHDGQVEIFDFCLNLLAEIAPVNWYAQTLPEEMRGCAIGGKDAYAFDRKNET
                     ITLNVPTAVYKLLPLSLTSASGAVGRVMLPLSSGESFYADRG"
     gene            4075..>4198
                     /locus_tag="PK442_03250"
     CDS             4075..>4198
                     /locus_tag="PK442_03250"
                     /inference="COORDINATES: protein motif:HMM:NF018498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HOO86694.1"
                     /translation="MKKAYVVGTCDTKFEELKFVADLIRQSGVEALLVDVGTKPH"
BASE COUNT          914 a         1261 c         1231 g          792 t
ORIGIN      
        1 gaaatacggg agcgtttcga gtcgtggacg aagcccgccc ccaaggtaac cagaggccat
       61 ctcgccctct actcgaagct cgcctcctcc gcggcgaagg gcgcgatcgt gcgcgccgag
      121 tgacggtggg acaataaaaa agggggcgga aaaccccgcc ccctcggttc ttatgcaatg
      181 ctgcgcttcc cctaccggat gaagcccaaa agccggggca aaaagagcga gatcggcgcg
      241 acgtaggtca caagagcaag gatggcgatc tccgcaagga cgaagcgcat caccgccgcc
      301 gaaacctttt ccaggctcag tccggtgatg ccgcagctca cgaagagaca gacccccacg
      361 ggcggcgtcg ccatcccgat ggcgaggttc agcaccatca tgaagccgaa gtgcagcgga
      421 tggacgccga gctgcgtcgc tatgggggcg aggatcgggg ccagcaggat gattgccgcg
      481 cccgtctcca tgaacatccc gacgatgagc agcaaaatat tcacgatgag aagaaagacg
      541 tacggattcg acgtcgcgga gaggaacatc gacgagagct tcaccgggat ctggttcgcc
      601 gcaatcaccc acgcgaagac gttggcgagc gagatgatca aaaggatcgc gccggtggtg
      661 atgccgctct ggaagagaat gcccggcagg tccttccacg taatctccct gaagacgaac
      721 ttcccgacga agaaggagta gatcaccgcg accgccgcgg cctccgtcgg agtgaagacg
      781 ccgccgagaa taccgcccag aataattacc ggggccatca gcgcccacac cgacgacttc
      841 agctctttcc tcaccctcgc cccggaaaag ccctccgaca tcgcgtagtt tttcttcctc
      901 gagacgcgcg aaacgacgat catcattgca aggccgagca tgatgcccgg aatgaacccg
      961 gcggcgaaga gaccgccgac ggaaacgttc accgagacgc cgtagatgac catgatgatg
     1021 ctcggaggga tgatcgggcc gatcgtcgcc gcggcgctgt tgagcgcggc gctgaagtcg
     1081 agatcgtagc cgttcttctc catcgagggg atcatgatgg agccgatggc ggaggcgtct
     1141 gccactgcgg agccgctgat accggcgaag atcatgttcg ccacgatgca ggcgtgggcc
     1201 agtccgcccc ggatatgccc cacaaggctg ttggcgaaac cgatgatgcg gactgtggtc
     1261 ccgccccggt tcatcaggtc gcccgcgagg atgaagaacg gcaccgccat gagcgggaac
     1321 gagtcgatcc cggtgaacat gcgctgcgcc acaagctgca gaggcactcc catcgcgagg
     1381 atgacggaga acccggtgat tcccatgacg agcgcgagag gaatccccat cacgaaacag
     1441 acgacgaagg ttatcgtcat aagcgcgatc atctgtcatc cccctttctc ttcagggcgg
     1501 cgtcgagctc tatatcggcg gtggcgagaa cctgagccga ccgggagagc atcagccgaa
     1561 gaagatgaag aaacatgtac acggcgccca cggggatcgc cagatacggc agccacatgg
     1621 gaaggtccat cgcgggcgac gtctgcaccc acaggcttac gatgagcttc acaccctgta
     1681 cggtcaccgc ggcaaggaag agcatgatca cagcctccgc cacgaagatg accttccgcc
     1741 gcaccccctc cggaagcttt tcaacgaaca tcgtgacgcc gatgtgcttc cccttcccca
     1801 tcgcgtatcc ggcccccagg aaggtcaccc acaccatcat gtaccgcgcg gcctcctcgg
     1861 accacgtgat ggggtcgtga aggacaaagc ggtagaagac cgccgcgaac gcgacgacgc
     1921 ccatgcttcc catcaggaga gcgaccgcgg cgaaggccgc cttgtcgaag cacgccaata
     1981 ctccctccaa aaacttcatt gctccacctc tccttcacag tgaaatggaa aaggggacgg
     2041 ggcaacaacg cgccccttcc ccgatgtgta ttcccgacga taaaagaatc cgtccggaag
     2101 ctacttcagc atctccgcca tcttcgcctc ggctgcggcg acggcttcaa gcgcttcgtt
     2161 aacccactgc tctcccgcct gcgccttgac gtagtcgagc acggcgggct gcgccgcgga
     2221 acggaactcg ttcagttcgg cggccgtggg aacgtagacg gtcattccgt ttttctgcac
     2281 gttcagaagg ccgctctgca cctgccgctc ggagatcgcg cgctgcgcga tgttgaagag
     2341 ctgcgcgccc tcgtagagcg cggccttctg gtcctgggag agcttctccc aaaccttgtc
     2401 gttgacgaac atgatcagcg ggttgtagag gtgtccgttg aggatcacat gcttctgcac
     2461 ctcgtagtac ttgccgttgt tgatcagagt cacgggcagc tcgcatccgt ccacgacgcc
     2521 ctgctgcaac gccatgtaga gttcgccgaa ggcgatgggg gtcggcgcgc cgccgagggc
     2581 ctcgaccatc ttcatgtgcg cggggttctc catcgtgcgg atcttgagcc ccttcatgtc
     2641 ggccatgctc tttacctgct tgctgccggt gacgaagtgg cggtagccgt tctcgctgat
     2701 cgccagagtc cgtatcccgg tgctcttgcg catctcctcc atcagcttgc ggccgaaggg
     2761 gccgtcgagc acttcccatg cgacaacctg gttggggaag aggaagggga tgtcgaagac
     2821 catgatgggc ttgaagagcg tcggcaccgg tccggtggtg atagtgccca gttcgatggt
     2881 cccgagcgcc agaccttcaa gaatctcctt ctcgttcccg agctgcgacg cgtggaagac
     2941 ctcgaccttg accgatccgc cggtcttcgt ttcgacgtgg ttcttgaaaa tctccaccgc
     3001 gatgaccgta cggtcctcgg gatcggcggg ccccgcgttc gccagcttca gcgtaaaatc
     3061 ggccgcgaga gcgcttcccg cgagaagtcc tgtaagagcg acaacacaca tgagtccaaa
     3121 catttttttc attttttacc ctccctctaa actctcataa atttatcccg gcattataaa
     3181 acaaagtttt tcctttgcaa aggggaatgt gttgtttcac ccccgatccg cgtagaagct
     3241 ctcccccgaa gagagaggca gcatcactct tcccaccgcc cccgacgccg aggtgagcga
     3301 aaggggcaga agcttgtaga ccgccgtcgg aacgtttagg gtaatggtct cgttctttct
     3361 gtcaaaggca taggcatcct ttcctccgat agcgcacccc cgcatctcct ccgggagcgt
     3421 ctgggcgtac cagttgacag gagctatttc ggcaaggaga tttaaacaaa aatcgaatat
     3481 ctcgacctga ccgtcgtgga gcatgcagat gttcaggaag acgttgacag ggtagtccag
     3541 gtagcggtgg ttccgtccga gcactctggt atgccttcct gcgacggtgt actcggcggt
     3601 gaagtccgga tcgttcggtt ttgaagccgc cttctccaga atttccatat tccagagaat
     3661 ctcggcgccc atggtggagc cgcagaatct cgggtagccg cttagctcct tgacgtaggg
     3721 aagcgcgccg ggaagcttgg gaagaggcgc tcccggacgg acctctatcg gacgaaaggc
     3781 tttctgtatt ctttcaagca gttccggcat gagactggcg ttcatgacga cacctcctgc
     3841 aaagcaagat aggcacagac tcttttcttt attctagcag tacaccgaaa gaataaccag
     3901 agagttacca aaatgtgtac tgcatccgat agcgcatcgt gaaaagagtt ccggtgcgca
     3961 gctcgatcac cccggaagcg ggaattttca cacagcaggg ggctgttaag ggtataatgt
     4021 ccctgtggtt tttccccgga caggggtatc ctgtgatgca gcaaggagga cggaatgaag
     4081 aaagcctatg ttgtaggaac gtgcgacacg aagttcgagg agctgaagtt cgtggccgat
     4141 ctcattcggc aatcgggcgt ggaggcgctc ctcgtggacg tggggacgaa accccacg
//
LOCUS       DAOSWW010000105         4160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14719_length_4160_cov_4.160292, whole genome shotgun sequence.
ACCESSION   DAOSWW010000105 DAOSWW010000000
VERSION     DAOSWW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14719_length_4160_cov_4.160292"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PK442_03255"
     CDS             complement(<1..950)
                     /locus_tag="PK442_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO86695.1"
                     /translation="MRNHQDTLRNEPVGSLLFRLSFPAALGMAVQASYSVVDAFFVGW
                     GVGHEGIAAVSMAFPLQMLLMAAAQTLGVGGASLLSRSLGAERRKRAERTLGTVFTCT
                     WILGILFAAVAIPSAPFVLYFLGAGEGVLPLASEYARALFRGAPFFAFSIVANNFVRA
                     EGNASFAMMTMLISAGINTLLDPLFIFGFEWGVRGAALATVAAQTATALWLAWYYLAR
                     KSAVRLRMKNLPPRAILLKETLSVGAAAFFRQAASSLSLIVVNGSLAALGGGAAVAAY
                     GIVNRLLLFAVMPVYGIVQGLLPIVGFNYGAKQFCRVLSA"
     gene            1085..2014
                     /locus_tag="PK442_03260"
     CDS             1085..2014
                     /locus_tag="PK442_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOO86696.1"
                     /translation="MQEVKKVITEVGTNEWQVVVFFLGDQTFAINVDKTREILRWPGC
                     RTIPESHPSMIGITSVRGEVLPLLDLRIHLGIEAKTPLDSSKVIIAEFNEIKLGFVVD
                     AVERIYRINSEELDSTLTGTFLGEYVLYVIKRDERNVLLLDYEAIVQMVNPTLSQRYT
                     IDVEKTAELTTPLGDLDQYRILVAEDSPLIRKQIADVLGQGGFHNLVLTSDGKEAWDK
                     LNTLGEHYDLLITDVEMPRLDGLALVRRVKEHNDLKKIPIVVFSSIMAQDIKVKAASV
                     GAEAQITKPEIPDMVEHVCRLLLANKEKKGSSK"
     gene            2011..2682
                     /locus_tag="PK442_03265"
     CDS             2011..2682
                     /locus_tag="PK442_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86697.1"
                     /translation="MTLLPYWDSNVDMEIASWFPLRMDYEPVSVNAHHSRLLSDIAAF
                     SLRMNSEFFCLTSLQSGYVGTLLRDSMIDPGRLNRIELGFTMLFGYSTSMESLLSSLL
                     DDESDVEAGVSFCCLWDRDIMLESFKAIVLVSSNPLFDEDEFLSRLAALLEKNSVPSQ
                     ANLPFFAEKFGYRRFFIPYETDNLVHLEISRLLYEMEFEETRKWVGPSPEVAFPPFFG
                     INLTV"
     gene            2796..3998
                     /gene="rpoD"
                     /locus_tag="PK442_03270"
     CDS             2796..3998
                     /gene="rpoD"
                     /locus_tag="PK442_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOO86698.1"
                     /translation="MENDMLNEKEILEDKEILEEKDVSKKDELSREDMAEYLGRVREL
                     IAEGHGKGFVTQKDIERHIPLENWSSEILDNVVSNLMELGIEVMDDDAKEPAPAQASA
                     AAADDDAVAPPSYDLELGKLDDIPLTDPVRMYLREIGKVPLLDAAEEVELAKRVEAGE
                     DLAKKKIVDANLRLVVSIAKKYIGRGMLFLDLIQEGNLGLIRAVEKFDYRKGFKFSTY
                     ATWWIRQAITRAIADQARTIRVPVHMVETINKMVRISRQLVQRLGREPTDDEIAAEME
                     IEPIKVEEIRRIAQLPVSLETPIGEEEDSQLGDFIEDRELPNPEEAAAGHLLHEQIEE
                     MLDALSEREREVLHYRFGLEDGRSYTLEEVGKRFGVTRERIRQIEAKALRKLRHPSRS
                     KKLKDFLD"
BASE COUNT          935 a         1265 c         1217 g          743 t
ORIGIN      
        1 atcgcggaga gcaccctgca gaactgcttc gccccgtagt tgaaccccac gatcgggagc
       61 agcccctgga cgatgccgta gacaggcatg acggcgaaga gcagcagccg gttcacgatg
      121 ccgtaggcgg ccaccgcggc gccccctccc agcgcggcaa gcgatccgtt caccacgatg
      181 agggagaggc ttgaggccgc ttggcgaaag aacgccgcag ctcccacgga gagcgtctct
      241 ttcaggagga tggcgcgcgg ggggagattc ttcatccgca gcctcactgc gctttttcgg
      301 gcgagatagt accacgccag ccataatgcc gtcgccgtct gcgccgcgac cgtggccagg
      361 gccgcgccgc gcacccccca ctcgaagccg aagatgaaca gcgggtcgag cagcgtgttg
      421 atccccgccg aaataagcat cgtcatcatt gcgaacgacg cgttcccctc ggcccggacg
      481 aagttgttcg ccacgatgga gaacgcgaaa aatggcgcgc ccctgaacag agcccgcgca
      541 tactcggagg cgagcggaag caccccctcc cccgcgccca ggaagtagag gacgaacggc
      601 gcggacggaa tcgcgacggc ggcgaagaga atccccaaaa tccacgtgca ggtgaagact
      661 gttccgagcg tccgttccgc acgcttcctt cgttccgcgc cgaggctccg cgaaagaagc
      721 gacgccccgc cgacgccgag agtctgggcg gccgccatca gcagcatctg aagcggaaag
      781 gccatggaaa cagcggcgat tccttcgtgc cccacgcccc agccgacgaa gaaagcgtcc
      841 accacgctgt acgaagcctg cacggccatg ccgagcgcgg cgggaaagga aagccggaac
      901 aacagcgaac ctacaggttc gttgcgcaga gtatcctggt gattccgcac ccgcattccc
      961 ctttctcaat ttgaattcga ttccaagtat agcagaagta ccgaggaggg gcttgtggtc
     1021 cgagccttca tgggatatag taagcacgga aaacaatccg ttccgttcgg aggagggagc
     1081 gtccatgcag gaagttaaga aagtgatcac cgaggtggga accaacgaat ggcaggtcgt
     1141 ggtgttcttt cttggggacc agacctttgc aatcaacgtg gacaagacca gggagattct
     1201 tcgttggccc ggatgccgca ccatccccga atcccatccg tccatgatcg gcatcacttc
     1261 ggtccgcgga gaggtgcttc cccttcttga tctgcgcata catctcggca ttgaagccaa
     1321 gaccccgctc gactcgagca aggtcatcat cgctgaattc aacgagatca aacttggatt
     1381 cgtggtggac gccgtcgagc ggatctatcg gatcaactcc gaagagctcg actccacact
     1441 gacgggaacc ttcctgggag agtatgtcct ctacgtcatc aagcgcgacg aacggaacgt
     1501 gctgcttctt gactatgagg ccatcgtcca gatggtcaac ccgacgctct cgcagcgcta
     1561 caccatcgac gtcgaaaaga ccgccgagct gacgacccct ctcggcgatc tcgaccagta
     1621 ccgcattctc gtcgcggagg actcgccgct catccgcaag cagattgccg acgtcctcgg
     1681 gcagggaggc ttccacaacc tcgtgctcac cagcgacggc aaagaggcat gggacaagct
     1741 gaacacactc ggcgagcact acgacctgct catcaccgac gtcgagatgc cccgcctcga
     1801 cgggcttgcg ctcgtccgcc gcgtcaagga gcacaacgat ctgaagaaga ttccgatcgt
     1861 cgtcttttcg tccatcatgg cgcaggacat caaggtcaag gccgcgagcg tcggcgccga
     1921 agcgcagatc acgaagcccg aaattccgga tatggtggag cacgtgtgcc gccttctgct
     1981 tgcgaacaag gagaagaagg gatcgtcgaa atgaccctgc ttccctattg ggattccaac
     2041 gtcgacatgg agatcgcttc gtggtttcct ctcaggatgg actacgagcc ggtctccgtc
     2101 aacgcgcacc acagcaggct tttgagcgac atcgcagcct tctctcttcg aatgaacagc
     2161 gaatttttct gccttaccag ccttcagagc ggctatgtcg gcacgctgct ccgggacagc
     2221 atgatcgacc ccggacggct gaaccgcatc gagctgggtt tcaccatgct tttcggctac
     2281 tccacgtcga tggagagctt gctctcctcc ctgcttgacg acgagagcga cgtggaagcg
     2341 ggcgtctcct tctgttgcct gtgggacagg gacatcatgc ttgagagctt caaagccatc
     2401 gtccttgtga gcagcaaccc tctgttcgac gaagacgaat ttctctcacg ccttgcggcg
     2461 ctcctcgaaa agaacagcgt gccgtcgcag gcgaatctgc cttttttcgc cgagaagttc
     2521 ggctaccggc ggtttttcat cccctacgag acggataacc tcgtccacct ggagatcagc
     2581 cgccttctgt acgagatgga gttcgaggaa acccgcaaat gggtcggccc ctctccggag
     2641 gtggcgtttc ctcctttttt tggtatcaat ctgactgtat agtggtataa tatatgtccg
     2701 tcggaaaaga gaagaagaac acacgcgacg agccggtgtc ccgagaggaa cattaggccc
     2761 gtcattgtcg ctttgaagag gggactggtt gttcgatgga gaacgatatg ctcaacgaga
     2821 aggaaattct ggaggataag gaaatccttg aggagaaaga cgtctccaaa aaggacgagc
     2881 tttcgcggga ggatatggcc gaatacctcg gaagagtgcg ggaacttatc gccgaggggc
     2941 acggcaaggg gtttgtaacc cagaaggaca ttgagcggca tattcccctc gagaactgga
     3001 gctccgagat tcttgacaac gtcgtctcca acctcatgga gctcggaatc gaagtaatgg
     3061 acgacgacgc caaggaaccg gctcccgcac aggccagcgc cgcggcagca gatgatgacg
     3121 cagtggcgcc gccctcctac gacctcgaac tcggtaagct ggacgacatc cctcttaccg
     3181 acccggttcg catgtacctc cgcgagatcg gcaaggtgcc gctgctcgac gccgccgagg
     3241 aggtcgaact ggccaagcgc gtggaggccg gagaagattt agcgaagaag aagatcgtcg
     3301 acgccaacct gcgccttgtg gtgagcatcg ccaagaagta catcgggcgc ggaatgctct
     3361 tcctcgacct catccaggag gggaacctcg gcctgatccg cgcggtggag aagttcgact
     3421 accgcaaggg gttcaagttc agcacctacg ccacatggtg gattcgccag gccatcacca
     3481 gggcgatcgc cgaccaggcg cgcaccatcc gcgtccccgt ccacatggtg gagaccatca
     3541 acaagatggt ccgcatctcc cgccagctcg ttcagcgcct ggggcgcgag ccgaccgacg
     3601 acgagatcgc cgccgagatg gagatcgagc cgatcaaagt ggaggagatc cgccggatcg
     3661 cgcagctccc cgtctccctc gaaacgccca tcggcgagga ggaagacagt cagctcggcg
     3721 acttcatcga ggacagggag cttcccaacc ccgaggaggc cgcagccggg cacctgcttc
     3781 atgagcagat cgaggagatg ctcgacgccc tctccgagcg cgagcgcgaa gtgctgcact
     3841 accgcttcgg cctggaagac ggccgttcgt acactttaga ggaagtcggc aagcgcttcg
     3901 gcgtcacacg cgaacgtatc cgccagatcg aagccaaggc gctgcgtaaa cttcgtcacc
     3961 ccagccgaag caagaagctg aaggatttcc tcgactaacc gctgaaagag agcgccgacg
     4021 cgtacccgga tcctctcacg agagtccggg tactttttct cgatacgtgc gccggctgct
     4081 ataataaaac ccggaaacga atgttctctc gagacaagga ggagtgatga agtgaggctc
     4141 ttttccatag tgattatctg
//
LOCUS       DAOSWW010000106         4150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14772_length_4150_cov_3.928205, whole genome shotgun sequence.
ACCESSION   DAOSWW010000106 DAOSWW010000000
VERSION     DAOSWW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14772_length_4150_cov_3.928205"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..488)
                     /locus_tag="PK442_03275"
     CDS             complement(213..488)
                     /locus_tag="PK442_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86699.1"
                     /translation="MILKIDFSRDAAKFYFNLQPKQFKQIAIALHDLTRDPEPNDSLA
                     MKGFPGFRRKDAGEFRVVYRVVNDTLMIDVIDKRNDDEVYKVFKRKM"
     gene            complement(485..736)
                     /locus_tag="PK442_03280"
     CDS             complement(485..736)
                     /locus_tag="PK442_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOO86700.1"
                     /translation="MITMTSTETRRTWGRVVEKARKNPVVVEKDGRPVVAVISYEDFE
                     RFQELEDRIWGERASAVREKGDYIGTEESMKLLQRGLEE"
     gene            complement(854..2953)
                     /locus_tag="PK442_03285"
     CDS             complement(854..2953)
                     /locus_tag="PK442_03285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO86701.1"
                     /translation="MSGKSTDVDALLAKATSPEGRMDMAKRWLDANLRKDPQLARRLI
                     KQLLPEAERNGDVLGKAWLLFQSGWLRLDADQYEQGCALLDSARSLFEAAGERAGVAR
                     CINALGRAYDRQGIFDLAIELYRESINLSEELGRSDIAGAASMNLAQSLFELGESGEA
                     LAVLEHCERHYSIAPHNRAVTHYQKGSAYRALGRLEEAERILMDGVGMAAGASRDAMD
                     MRCLLAETLMDRGRLDIALKLILKGLKDAAAFGERHVWIRFALAKTRFHLLQGSSDEA
                     ISVVQDARRAASELGARKLEAEAERLLYKAYKAAGAHEEALAAFERYSEMKDLARNEQ
                     AASRIFGLHTERARREARHFEALYRQIATISEVGQRIASHLDLESSLATIQNAINNLM
                     DASTVILALVDEEKQLLDYRYVICRGERRESFTRPLDMQSFGCWCVRNRSDIIIGDLE
                     SEYSRYVPSYKGLVFEGVPESSLVYIPLILEERVVGMLSVQSTRQHAYDQQKVKVLHA
                     IGGYIAISIENSRLFSQIERLASTDELTALPNRRTVMEELAKVCRRTRRYGRVSGVVM
                     ADVDHFKRINDMYGHDIGDVALRSIAGALASSVRESDMVGRFGGEEFVVVLQETDSKG
                     ALLLAERLRKTVEGLEISGPHGESLRVTASFGVSVVSQKDPDCHPVLKRADDALYEAK
                     ATGRNRVCSCLPEDGDA"
     gene            complement(3009..3305)
                     /locus_tag="PK442_03290"
     CDS             complement(3009..3305)
                     /locus_tag="PK442_03290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOO86702.1"
                     /translation="MILVSAKFKAKPGMREKIVEISRYAVECTRKEKGNISYTLYTST
                     DDEETLMYFEEWTDIESLRAHLKTQHILDAREARKEMLAAPVEVKVFESKEVAL"
     gene            complement(3573..>4150)
                     /locus_tag="PK442_03295"
     CDS             complement(3573..>4150)
                     /locus_tag="PK442_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO86703.1"
                     /translation="EYVSFGSLEGVDEHVVIMDAVSKRYSACGARVGCLVSRNAGLVA
                     QVLKLCQARTCVGAMDMAGAAALYDTPVSYLKDVNDEYTARRNTVYRALKQIPGVVCE
                     EPKGAFYVVAKLPVDDAEKFIIWMLGSFNVGGETVMMSPAEGFYATPGLGRDEVRIAY
                     VLKNEELAKAMDILKAGLEAYPGRVSPVAAG"
BASE COUNT          860 a         1307 c         1055 g          928 t
ORIGIN      
        1 gagcctcgga gccgtgccat tcgacggcgg ccacggtcac aagcgcgtcg ggcagtatcc
       61 ctcggacgac cgtctcgggg tgcagatctt cgagtcgcaa agcgtcacct tccttgcagg
      121 aatgagttcg cacacaagaa acacgcgttc gctcttttcg atgcggagtt tcaggccctc
      181 ttcttctgag aaaagaggat gatacgaatt ccttacattt ttcttttgaa caccttatac
      241 acttcatcgt cgttgcgctt atcgatcacg tcgatcatga gcgtgtcatt cacaaccctg
      301 tagaccacgc gaaattcccc cgcgtctttt cgacgaaagc ctgggaaacc cttcatcgca
      361 agagaatcgt tgggttccgg atcgcgagta aggtcatgca aggctatcgc tatctgtttg
      421 aactgctttg gctgcaagtt aaagtaaaat ttagcagcat ctctggaaaa atcaatttta
      481 aggatcattc ttccaagccg cgttgcagca gcttcatgct ttcttccgta ccgatatagt
      541 ctcccttttc acggactgcg ctcgctcttt ccccccagat gcgatcctca agctcttgaa
      601 aacgttcaaa atcctcgtag gagatgacgg caaccactgg ccggccgtct ttttccacca
      661 ctacagggtt ttttctggct ttttcgacaa cgcggcccca agtacgccga gtctccgtgg
      721 atgtcatcgt aatcattgtt tatccccctc taatgctcac tatgaacact ttgatcaata
      781 taagcattat agcatcttaa aaaatacatg tacctccgtt cctccatcat gcaaaaagaa
      841 acgagcatgc tcactatgcg tccccatcct cgggcagaca gctacagact cggtttcttc
      901 cagtagcctt ggcctcgtag agggcatcat ccgcacgctt cagcacgggg tggcagtcgg
      961 ggtccttctg cgacaccaca gagacgccga aactcgccgt tacccgcagg ctctcaccgt
     1021 ggggtcccga tatttcgagt ccttcaactg ttttccggag acgttcggct aaaagaagcg
     1081 ctcccttgct gtcagtctcc tgcagaacga cgacgaactc ctccccgccg aaccgcccga
     1141 ccatatcgct ctcccgcacg cttgaggcga gcgcgccggc gatggaacgg agcgcgacat
     1201 cgccgatatc gtggccgtac atgtcgttga tccgtttgaa atgatccaca tcggccatca
     1261 ccacccccga aacccgcccg taacgcctcg tcctccggca gactttcgcc agctcctcca
     1321 tcacagtccg tcggttcgga agagcggtga gctcgtccgt cgaggccaga cgctcaatct
     1381 gactgaaaag acgcgagttt tcgatggaaa tggcgatgta gccgccgata gcgtgaagaa
     1441 ccttcacctt ctgctgatcg taggcgtgct ggcgggtgct ctgcacgctc agcattccca
     1501 cgactctctc ttcgagaata agcgggatgt agaccagcga cgattcagga acgccctcga
     1561 aaaccagtcc cttataggag ggaacgtacc ggctgtactc cgactccaga tcgccgatga
     1621 tgatatcgct ccggtttctg acacaccagc acccgaacga ctgcatatcc agaggacggg
     1681 tgaaagactc gcggcgctct ccacggcaaa taacgtaccg gtagtccagg agctgttttt
     1741 cctcgtccac aagcgcgaga atcaccgtcg acgcatccat caggttattg atggcgtttt
     1801 gaatcgttgc gagcgagctc tccagatcca aatgcgacgc gatgcgctgg ccgacttcgc
     1861 tgatcgtagc gatctgccgg tagagcgcct cgaagtggcg cgcctccctg cgggcgcgtt
     1921 ccgtgtgcaa cccgaaaatt ctgctcgcgg cctgttcgtt acgcgccaga tccttcatct
     1981 cggaatagcg ctcgaaagcg gccagggctt cttcgtgtgc tcctgcagcc ttgtacgcct
     2041 tgtacagaag gcgctccgct tcggcttcca gtttgcgtgc tcccagttcc gacgccgctc
     2101 ttctcgcatc ctgaacaaca gaaattgctt catcagagct cccttggagc aaatggaaac
     2161 gggttttcgc gagtgcaaac ctgatccata cgtgccgttc gccgaaagca gcggcatcct
     2221 tgagcccttt gagtataagc ttcagggcga tatcgagccg tcctctgtcc attaaggttt
     2281 ccgccagaag acagcgcata tccatggcat cacgcgaagc tcccgcagcc atgcccaccc
     2341 cgtccatcag gatgcgttcg gcctcctcca gcctccccag cgcgcggtac gcgctcccct
     2401 tctgataatg cgtaacggcc ctgttatgag gggcgatgga ataatggcgc tcgcagtgtt
     2461 ctaaaacagc gagcgcttcc ccggattcgc cgagttcgaa gaggctctgc gccaggttca
     2521 tagacgccgc cccggctata tccgaacgtc ccaactcttc ggacaggttg atcgactccc
     2581 ggtataattc gatcgccaga tcaaaaattc cttgccggtc gtacgcgcgc cccagggcgt
     2641 taatacatcg agccaccccg gcgcgctccc cggcggcttc gaacagagat cgagccgaat
     2701 caagaagggc gcacccctgc tcgtactggt ccgcatccag acggagccac cccgactgaa
     2761 aaagaagcca ggccttcccc aaaacatctc cgtttctctc tgcttcaggt aaaagttgtt
     2821 taatcaatct acgagccagc tgcggatctt tgcgaaggtt cgcgtcaagc cagcgtttgg
     2881 ccatatccat acgcccctcc ggagaagtcg ccttcgctag cagagcatcc acatccgttg
     2941 actttccact catgtccgtc acctcgcact cctcgcagct gctgccatca tgacaaagct
     3001 ccgctctctt agagcgcgac ctccttcgac tcgaagacct tgacctcaac tggagctgcc
     3061 agcatctcct tccgcgcttc gcgggcgtcg aggatatgct gcgtcttcag gtgcgccctc
     3121 aaggattcga tatccgtcca ctcctcgaaa tacatgagcg tctcttcgtc gtcggtggac
     3181 gtgtacagag tatagctgat gttccctttc tccttccgcg tgcactctac agcgtaccgg
     3241 ctgatttcga caatcttttc cctcattccg ggctttgctt tgaacttcgc gcttacgagt
     3301 atcatgccga ttccaccttt cctggcgagt attctcacgg aggcgcaacg gacgatttta
     3361 ggagtctccg cgatggtaat accgttactg aagcatcata tcatacggtg cgcagcttcc
     3421 ggagattttt tacacactac ttcagggaga ggcgatgacg gggaaaacca aagagcgccc
     3481 cgccccatct tcgggcgagg acgctctctt cggataacaa ctgaatccgg cgccgtcccg
     3541 tggttacgag aggaaacgct gcgaaaacgc ttctaccctg ccgcgacggg cgagactctg
     3601 ccggggtagg cctccagacc tgccttgaga atatccatgg ccttcgccag ctcttcgttc
     3661 ttgaggacat aggcgatgcg gacctcgtcg cgtcccaggc cgggtgtggc gtaaaagccc
     3721 tcggcggggg acatcatcac cgtctctccg ccgacgttga aactgccgag catccagatg
     3781 atgaacttct ccgcgtcgtc cacgggaagc ttggccacga cgtagaaggc gcccttcggc
     3841 tcctcgcaga caacgccggg aatctgcttc aacgcgcggt agaccgtgtt gcgccgggcg
     3901 gtgtactcgt cgttgacatc cttcagatac gaaacggggg tgtcgtagag cgccgccgcc
     3961 ccggccatgt ccatcgcgcc gacgcaggtg cgggcctggc aaagcttcag tacctgcgcg
     4021 acaagtccgg cgttccggct caccaggcag ccgacgcggg cgccgcacgc gctgtagcgc
     4081 ttggagacgg cgtccatgat gaccacatgt tcgtccaccc cttcgaggct gccgaagctg
     4141 acgtactcca
//
LOCUS       DAOSWW010000107         4143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14814_length_4143_cov_3.865460, whole genome shotgun sequence.
ACCESSION   DAOSWW010000107 DAOSWW010000000
VERSION     DAOSWW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14814_length_4143_cov_3.865460"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PK442_03300"
     CDS             complement(<1..200)
                     /locus_tag="PK442_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO86704.1"
                     /translation="MDEQKSEMPIGKVLPLPLVEEIKHSYLDYAMSVIVGRALPDVRD
                     GLKPVQRRILYAMMELGLRSGSS"
     gene            complement(337..864)
                     /locus_tag="PK442_03305"
     CDS             complement(337..864)
                     /locus_tag="PK442_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO86705.1"
                     /translation="MTLRDNSVIRAILSRRSIRKFRSDAVSPEMRDLLLECAFAAPSA
                     HNRRPCHFIVLEDRALLDAVAEVHDSGKMLFQAPLAIAVCAETASYPEGDLAWVEDCS
                     AALENILVAAKGLGLDGVWLKVMGRHPRNELVRPVLGVPESVQIVGIAALGFGAEEKS
                     PCEGIKEDRVHKNRW"
     gene            956..1474
                     /locus_tag="PK442_03310"
     CDS             956..1474
                     /locus_tag="PK442_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOO86706.1"
                     /translation="MSKKIAVLTEETIHDLEFWYPYYRLAEEGYDLVIVGPEAGKSYT
                     GKMGGVIRSEKSPSEISAKDFAGVVVPGGWGPDKLRTHKAIVDFVRDVHANGGVVAAI
                     CHGGSVLVSARILEGVRATSYKSIKDDMILAGAEWVDEAVVVSKNIVTSRTPSDLAHF
                     GKALVETLKKIK"
     gene            complement(1467..2108)
                     /locus_tag="PK442_03315"
     CDS             complement(1467..2108)
                     /locus_tag="PK442_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO86707.1"
                     /translation="MGERENTEARILDAVGTILSRKGFRYLGVNAIAREACVDKVLIY
                     RYFGGLQPLLRTFFTRRKYWPTTSEILETAGEGEADPATRAYAILRGYLRELKKSDAA
                     REVLRGEIAGREDVASETAEERYRQGMELLDLLREPDKETEGLTEMAALLSAGFTFLL
                     LRKDVSSRYLGLDLRNEETWERLEKAIHLLVKLYYKEKAPPEGRGLAGQEETT"
     gene            complement(2174..3835)
                     /gene="ilvD"
                     /locus_tag="PK442_03320"
     CDS             complement(2174..3835)
                     /gene="ilvD"
                     /locus_tag="PK442_03320"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOO86708.1"
                     /translation="MRSDNAKKGAERAPHRSLLKASGYTDWEIARPWVGVVNPYNALI
                     PGHIHLNRIAEAVKSAVYAFGGFPLEFPVIGVCDGIAMNHEGMKFSLPSRELIMDSIE
                     VMVRAHALDALVLVTNCDKIIPGMAMAALSLNIPTIMISGGPMLAGTTGGKDVDLSNI
                     FEAVGKRAAGLISDEELREIEDITCPTCGSCAGMFTANTMNCMIEALGFALPGNGTIP
                     AVYSARDRLAKEAGRQIMTLVERNIKPRDILTEKAFDNAVAVDMALGGSTNTALHLPA
                     LAHAAGLSLTLDRFDEISARTPHLCSMSPGGPHHIQDLYAAGGVQAVMARLVQGGLID
                     GNSLTVTGKTVAENLEGVVVKDDAVIRPLDRPYHAQGGLAVLKGNIAPEGSVVKQSAV
                     DPSMLKHSGPARVFDSEEAASKTILEKGIKDGDVVVIRYEGPKGGPGMREMLTPTSSL
                     AGMGMDKTVALITDGRFSGASRGASIGHVSPEAAAGGMIGLVKEGDIIEIDIPARKLH
                     LAVPEDEIARRRSEWKPVETQVDSLFLNRYREFVTSGSKGAVFSD"
     gene            complement(3871..>4143)
                     /locus_tag="PK442_03325"
     CDS             complement(3871..>4143)
                     /locus_tag="PK442_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO86709.1"
                     /translation="IESPVTNCCSHGNLLHFGVLIAIGLAIGMATPPVAINIYVAGAI
                     TGLPMEQISKPIIPMVFVLIAILLLVTYVPSLILSLPMLVWGPGGI"
BASE COUNT          884 a         1243 c         1150 g          866 t
ORIGIN      
        1 gaggaccctg accggagccc cagctccatc attgcgtaca ggattcgccg ctgcactggt
       61 ttcagaccgt cccgcacatc ggggagcgcg cgccccacga tgacgctcat cgcgtaatcg
      121 agatagctgt gtttgatctc ctctaccaga ggaagaggga gcaccttgcc aatgggcatt
      181 tcactttttt gttcgtccat acgatccttc aacctccaaa gctgattcgc cgtgcaaaaa
      241 gccggcgacg tgtacagcat aacctgtata ttgtatcacg tcaggaggag tagtgagctg
      301 tcccaacacg catgaatgct cttcccgggt gtttatttac caacggtttt tgtgcactcg
      361 gtcttctttg attccttcgc agggactttt ttcctccgca ccgaatccga gcgcggcgat
      421 acccacaatc tgcaccgatt ccggtacgcc gagaacagga cgcaccagtt cgttccgcgg
      481 gtgacgcccc atcactttca gccagacgcc gtcgaggcca agcccctttg cggcgacaag
      541 gatgttctca agagccgcag agcagtcctc cacccaggca agatctcctt caggatagga
      601 tgcggtttcg gcgcagacgg cgatcgccag gggcgcctga aaaagcattt tccccgagtc
      661 gtggacttcc gccaccgcat cgagcaaagc ccgatcctcg agaacaataa agtggcacgg
      721 acgccggttg tgcgccgacg gcgcggcaaa tgcgcactcc agaagcaggt cgcgcatctc
      781 cggcgataca gcatcggagc gaaacttcct gatactcctc cgggagagaa tcgccctaat
      841 cacagaatta tccctcaatg tcattgtcat cgcctcctgt aaaggatgtt ttatgataga
      901 ctataaacgt gaagagtttc aacaatcgta tcaccaaaag aaggagggac agagtatgtc
      961 caagaaaata gccgtactta cagaggagac cattcacgac ctcgaattct ggtaccccta
     1021 ctaccgtctt gcggaagagg ggtacgatct cgtcatcgtc ggcccggaag cgggaaagag
     1081 ctataccggg aagatgggcg gcgtgatccg ctcggagaaa tcaccttcgg agatctccgc
     1141 gaaggacttc gcaggcgtcg tcgtccccgg agggtggggc cccgataagc tcagaacaca
     1201 caaggcaatc gttgatttcg tcagggacgt ccatgccaac ggcggcgtcg tcgcggcaat
     1261 atgccacggc ggcagcgtcc ttgtatcggc gcgtattctg gaaggcgttc gcgccacttc
     1321 ctacaagagc atcaaagacg atatgattct cgccggagcg gaatgggttg acgaggcggt
     1381 agtcgtctcg aagaatatcg tcacaagccg cacgccttcc gatctggcgc acttcggcaa
     1441 ggcgctcgtc gaaacgctca aaaagatcaa gtagtttctt cctgtcctgc aaggcctcgc
     1501 ccctccggcg gggccttttc cttgtaatac aacttcacaa gaaggtgtat ggctttctcc
     1561 agtcgctccc acgtctcctc gttccgcagg tcgagcccga gataccggga ggatacatcc
     1621 tttcgcagca gcaagaaagt gaatcccgca gagaggagcg cggccatctc ggtcagtcct
     1681 tccgtctcct tatcgggttc acgaagaagg tcgagcagct ccatcccctg ccggtaacgt
     1741 tcttccgccg tttccgaagc gacgtcttct cttcctgcaa tttctccccg cagaacttca
     1801 cgggcagcat ccgacttttt cagctcccgg agataccccc tgaggatcgc gtacgccctc
     1861 gtcgccggat cggcctcgcc ttcaccggcg gtctccagga tttccgaggt tgtcggccag
     1921 tacttcctcc gcgtgaaaaa cgtccgcaga agcggttgaa gccctccaaa ataacgatag
     1981 atgagcacct tgtccacaca agcttcccgg gcaatggcgt tcaccccaag gtaccggaaa
     2041 ccttttcttg aaaggatggt ccctaccgcg tcgaggatgc gggcttcggt attctccctc
     2101 tcccccacat tcttttcccc ctttggaaag cgagaagggg cttccggaga aagccccttc
     2161 acagcaaaaa tcattagtcg ctgaacaccg ctcctttgga gccggatgta acaaactcgc
     2221 gatacctgtt gaggaaaaga ctgtcgactt gggtctctac aggtttccat tccgatctgc
     2281 ggcgggcgat ctcgtcttca ggaacggcga ggtgcagttt ccgtgcgggg atgtcgatct
     2341 cgatgatgtc tccttctttc acgaggccga tcattccgcc cgcagccgcc tccggagaga
     2401 catgaccgat agatgcgccg cgtgaagcgc cggagaaccg tccgtcggtg atgagcgcaa
     2461 cggtcttatc catccccatt ccggcgagcg aagaggtcgg agtaagcatc tcgcgcatcc
     2521 ccggtccgcc cttggggcct tcgtaacgga tgacaaccac gtcgccgtcc ttgatcccct
     2581 tctcaagaat ggtcttcgac gccgcttcct cgctgtcgaa gacgcgggcc ggaccggagt
     2641 gcttcaacat tgaaggatca acggcggact gcttgacaac gctcccctcc ggagcgatgt
     2701 ttcccttaag cacggcaaga ccgccctggg cgtggtacgg cctgtcgagc ggacggataa
     2761 cagcgtcgtc ctttacaacg accccctcga ggttttcggc tacggtcttc cctgttactg
     2821 taagcgagtt cccgtcgata agaccgccct gcacaagacg cgccatcacg gcctggactc
     2881 cgcctgcggc gtagaggtcc tggatatggt gcggcccgcc ggggctcatg ctgcacagat
     2941 gcggcgtacg agcgctgatc tcgtcgaacc tgtcgagcgt cagcgaaaga cctgcggcat
     3001 gcgccaatgc cggaagatgg agcgcagtgt tggtagagcc gccgagcgcc atatcgacgg
     3061 caacggcgtt gtcgaaggcc ttctccgtga ggatgtctct gggcttgatg ttcctctcga
     3121 ccagcgtcat gatttgccgt ccggcctcct tggccagtcg gtcccgggcc gagtagacgg
     3181 caggaatggt tccgttcccg gggagagcaa aaccgagcgc ctcaatcatg cagttcatgg
     3241 tattcgcggt gaacataccg gcgcacgaac cgcatgtcgg acaggtgata tcctctatct
     3301 cgcggagctc ctcgtcggaa atcaacccgg cggccctctt gccgacagcc tcgaagatgt
     3361 tgctgagatc gacgtccttg cctcccgtcg ttccggcgag catcggcccg ccgctgatca
     3421 tgatcgtcgg gatgttcagc gagagcgccg ccatcgccat gccggggata atcttgtcgc
     3481 agttggtgac gagaacgaga gcgtcaagcg catgcgcccg caccatcacc tcgatggaat
     3541 ccatgatcag ctcgcggctg gggagcgaga acttcatccc ctcgtgattc atggcgatgc
     3601 cgtcgcagac gccgatgacg gggaattcca gagggaagcc cccgaatgcg tacacagcgg
     3661 atttcactgc ctccgcgatc cggttcagat gaatatgacc gggaataagc gcgttatagg
     3721 ggttgaccac gcccacccag ggacgggcga tctcccaatc ggtatatccg gacgctttca
     3781 acagcgagcg gtgaggcgcc cgttccgcgc ccttctttgc gttgtcgctt cgcataacaa
     3841 ttacctcctc ttgaaattac ttttgaagcg ttatattcct cccggtcccc agacgagcat
     3901 tggaagagag aggatgagcg atggcacata ggtcactaaa agaagaatag cgatgaggac
     3961 aaaaaccatc ggaatgatcg gcttgctgat ctgctccatt ggaagcccgg taatggctcc
     4021 tgcaacgtag atatttatcg cgacaggggg ggtagccatt ccgatggcaa gccctatggc
     4081 gatgagcacg ccgaagtgga gaaggtttcc gtgagaacag caattggtca cgggtgactc
     4141 gat
//
LOCUS       DAOSWW010000108         4135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14862_length_4135_cov_4.594608, whole genome shotgun sequence.
ACCESSION   DAOSWW010000108 DAOSWW010000000
VERSION     DAOSWW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14862_length_4135_cov_4.594608"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..244
                     /locus_tag="PK442_03330"
     CDS             104..244
                     /locus_tag="PK442_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86710.1"
                     /translation="MPRLGRRGLSAVERNARSRLLRLIHNSLLLHGSLVTMARMTAPV
                     GN"
     gene            complement(358..1167)
                     /gene="istB"
                     /locus_tag="PK442_03335"
     CDS             complement(358..1167)
                     /gene="istB"
                     /locus_tag="PK442_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOO86711.1"
                     /translation="MTKRPEKPSNEELHDRILGHFAVLCVPVKGEDIDAAVTQAERHR
                     LSHLEFLESLIGAQANRRRERAIERRIHEARFSAVRLLETFDWEFNRRAIDRTQMEAL
                     ATCDFVRRRENIVVIGQSGVGKSHLMQAIGRRGCVLGYRVRYITSAALLQDLIAGLAD
                     KTLPQRLRRYDRYELLIIDEFGLDKIERQESPVASSLMYKVIDARRCKLSTIVITNID
                     FDAWGSYLGDPPLAMAVLDRVVDNATVIKINGRSYPSAKAKRASAPPAAEK"
     gene            complement(1151..2659)
                     /gene="istA"
                     /locus_tag="PK442_03340"
     CDS             complement(1151..2659)
                     /gene="istA"
                     /locus_tag="PK442_03340"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOO86712.1"
                     /translation="MSLRSKRVPIRGIAKQLGISRNTVRSVLKSVQKERDEGAAHPDL
                     ALAVRRPSCLDAHEERIRELLDRYQDITAQRVLEELREAGFTGGYTIVKERVRELRPR
                     PLTEPSLRFETKPGYQAQMDYSPFTIAFTEEGLRRVYLFSYILGYSRRRYHHWVEAQD
                     FTTTVREHVRAFKYLGGIAEVCLYDGMKVVVLRYEDEEPIYNPRFLAFCTHYGYRPWA
                     CRRRRPQTKGKVERPFLFVDTNLLNGRTFRSLAHLNEVTEWWLANVADVRPNAETNRP
                     PIEMYEEERAHLLALPAHDYDTAEVAYRVVNAEGGVHYLGNFYSVPWQYIGSLLPVKI
                     TETALMVYSPKITEIARHALVPRGITGTRCIDKAHTPGDDLRKKHELLKSRYEEFGQR
                     GVRFLEGLVAHRRYGKDEAWKVLMLLGTYQRADFVAALERAVRFGAYSVHAVERILAA
                     NAVPKPPIEVVGEREARRISDCFGDTPIVPRSTKDYEEIYPPTKDGHHDEAP"
     gene            complement(2643..3638)
                     /locus_tag="PK442_03345"
     CDS             complement(2643..3638)
                     /locus_tag="PK442_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOO86713.1"
                     /translation="MGDGASRGGAWDFMGSWEAGAVHPVALTDIGERYARYRLHDPEA
                     TKVMEKSLRRYGQISPVVVCLRDGGLELVDGFKRLLAARAIPRMSTLAARLLALDERT
                     AKAAIYSLNRVGRRIQELEEAWLVYALVREDGLTQVETAELLGRHKSWVCRRLALLER
                     LAAEAKDGLRLGLLSPTAARQLTRLPAGNQVEVLAAMRREALCAAEVRQVVDLFLASA
                     DTTQREFVLRKPREAIGQAHAEVVPTSDPRLTVAGNRLSKDLAILLDRLARMATWFQF
                     RGLAELSRGDRGILAPHVERLVSEARVMADLAEEFHTEVVRHEAGAAQRDCVLTQ"
     gene            3935..>4135
                     /locus_tag="PK442_03350"
     CDS             3935..>4135
                     /locus_tag="PK442_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86714.1"
                     /translation="MQKLPNSHLEGYPRGALARDRIRSIAAGPAPAYDGPVFVPFDGE
                     TALSGAACPSPWSGISFACAIAA"
BASE COUNT          733 a         1418 c         1184 g          800 t
ORIGIN      
        1 tccacgagaa aacaagcaaa ttcgcattca aaagaagctt gactttttac cctgaagcag
       61 aaataagcgc aaatatcaga ttgatctcca gggagacttg tttatgccaa ggctcggtcg
      121 acgcggtctt tcggcggtgg aacgcaacgc gcgctcccgc ctcctccgcc tcatccataa
      181 ctcacttctc ctgcacggga gcctcgtcac catggcgaga atgacggcgc cggtcggaaa
      241 ctgacccacc ttggcggtga gaattgaccc acctgcgcgg ttgcgtactg gaattcgcgc
      301 ggcggatttg cctgaagggg agccgatggc ggcgctgcgt ccagtgggcg ccgggcgcta
      361 tttctccgcc gccggtgggg cggaagccct cttcgccttt gcggaagggt acgatctgcc
      421 gttgatcttg atgacggtgg cgttgtcgac gacgcgatcg agcaccgcca tcgccaacgg
      481 cggatcgccg agataacttc cccaggcgtc gaagtcgatg tttgtgatga cgatggtcga
      541 gagcttgcag cggcgcgcat cgatgacctt gtacataagg ctcgaggcca ccggactctc
      601 ctgccgctcg attttgtcca ggccgaattc gtcgatgatc agcagctcat agcggtcgta
      661 acgccgcagc cgttgaggaa gcgtcttgtc ggcaaggccg gcgatgagat cctggagcag
      721 cgccgcgctc gttatgtacc ttacccgata acccagcacg cagccccgcc tcccgatcgc
      781 ctgcatcaag tggctcttgc cgacgccgct ttggccgatg acgactatgt tctcgcgccg
      841 gcggacgaaa tcgcatgtcg ccagcgcttc catctgcgta cggtcgatcg cacgccggtt
      901 gaattcccaa tcgaaggtct caagcagcct gactgcgctg aagcgagcct cgtggattcg
      961 ccgttcgatg gcacgttccc gccgcctgtt ggcttgtgcc cctataagtg actccagaaa
     1021 ctccaggtgc gacaaacgat gtcgttcggc ctgtgtgacg gcggcatcga tgtcttcgcc
     1081 cttcaccggc acgcacagga cggcgaagtg ccccagaatg cggtcgtgca gttcctcgtt
     1141 actcggcttt tcagggcgct tcgtcatggt gcccgtcctt cgtaggcgga tagatctctt
     1201 cgtagtcctt cgtcgaacgc ggcacgatgg gtgtatcgcc gaagcagtcg cttatccggc
     1261 gcgcctcgcg ctcacccacg acctcgatcg gcggcttcgg taccgcattg gccgccagaa
     1321 tgcgctcgac ggcgtgtacc gaataggcgc caaaacgcac cgcacgctcc aaggccgcga
     1381 cgaaatcagc acgctggtag gtgccgagaa gcatcagcac tttccaggcc tcgtcctttc
     1441 cataccggcg gtgggccacg agcccttcca ggaaacgcac tcccctctgg ccgaattcct
     1501 cgtaccgtga cttcagaagc tcgtgcttct tacgcagatc atcgccaggc gtgtgtgcct
     1561 tgtcgatgca acgcgttccg gtgataccgc gcggcacgag cgcatggcgc gcaatctccg
     1621 tgatcttcgg gctatagacc atgagcgccg tctccgtgat cttcaccggc aagaggctgc
     1681 ctatgtactg ccaggggacg gaatagaaat tccccaggta gtggacgccg ccctccgcgt
     1741 tcaccacacg gtacgccacc tccgccgtgt cgtaatcgtg cgccggcaac gcgagcaggt
     1801 gcgcgcgctc ttcttcgtac atctcgatcg gcggtcggtt cgtttccgcg ttcggtcgca
     1861 cgtcggccac gttggccagc caccattccg tcacctcgtt caggtgcgcc agcgaccgaa
     1921 acgtgcggcc gttgagcaga ttggtgtcta cgaagagaaa tgggcgctcg accttcccct
     1981 ttgtctgcgg ccgtcttcgc ctgcacgccc acgggcggta gccgtaatgc gtgcaaaagg
     2041 cgagaaacct gggattgtag atcggctcct cgtcctcgta cctcagcacc acgaccttca
     2101 tgccgtcgta caggcatacc tcggcaatgc cacccaggta tttgaaagct cgcacgtgct
     2161 cccgtaccgt cgtcgtgaaa tcctgcgcct cgacccaatg gtggtagcga cgccgcgagt
     2221 acccgaggat gtaactgaag aggtacaccc gccgcagccc ctcctccgtg aaggcgatcg
     2281 taaacggcga gtaatccatc tgcgcctgat accccggctt ggtttcgaaa cgcaacgacg
     2341 gctccgtcaa cggccgcggc ctcagctctc ggacccgttc cttgacgatc gtgtagccgc
     2401 cggtgaagcc cgcttcccgc agctcctcaa gcacccgttg cgccgtgatg tcctgatacc
     2461 tgtccaaaag ctcccgaatc ctctcttcgt gggcatccaa acagcttgga cgccgcacgg
     2521 ccagcgccaa gtccggatgc gcggcaccct cgtcccgctc cttctggacg gacttgagca
     2581 ccgacctgac cgtattgcgg gatattccca gttgcttcgc gatgccgcga atcggcaccc
     2641 gcttactgcg taaggacaca atctcgttgc gcagctcctg cttcatgccg tacgacctcc
     2701 gtgtggaatt cctcggccag gtccgccatc acccgcgctt cgctgacgag ccgttcgaca
     2761 tgtggcgcca gtatgccgcg atcgccccgg gacaattccg cgagccctcg aaactgaaac
     2821 cacgtcgcca tgcgcgcaag ccggtcaagc agaatcgcga gatctttcga gagcctgttc
     2881 ccggcgaccg tcaaacgcgg gtcggacgtg ggcacgactt ccgcgtgcgc ctggccgatg
     2941 gcctcacgtg gcttccggag cacaaactcc cgctgcgtcg tatccgccga cgccagaaag
     3001 agatcgacga cctggcgcac ttccgccgcg cagagtgctt cccggcgcat cgcggcgagc
     3061 acctcgacct ggttgcccgc gggcaaccgc gtcagctggc gcgcggccgt cggcgacagc
     3121 agccccagcc gcaagccgtc tttggcctcg gccgccagac gctcaagcag cgccaggcgc
     3181 cggcagaccc agctcttgtg ccggccgagc agctccgccg tctctacctg cgtcaatccg
     3241 tcttcccgca ccagtgcgta gacaagccac gcctcctcaa gctcttgaat ccgtcggccg
     3301 actcgattga ggctgtagat cgcagctttc gccgtgcgct cgtcgagcgc cagcagtctc
     3361 gccgcgagcg tcgacatccg cggaattgcg cgcgccgcca acaggcgttt gaagccgtcg
     3421 acgagctcga gccctccgtc gcgcaggcag accacgacag gcgagatctg gccgtagcgc
     3481 ctcagggact tctccatgac cttggtcgcc tccggatcgt gcaaccgata ccgcgcgtag
     3541 cgctccccga tatcggtgag cgccacgggg tgcaccgctc ctgcctccca actccccatg
     3601 aaatcccacg ctcctcctcg gcttgcccca tcgcccacta catttgagaa aggaagcggg
     3661 gggacctacg tcaggcccct cccgttccct tctcgtcttt tgggtagctc gacaatacgc
     3721 cccgggcagc agcggccgca atattcgtca cacagcgcag cgatggaatt ccttgccgcc
     3781 gcggctcact cccgccggca gcggtgcata aatccccaac gaatctcgcg ctcgaccacg
     3841 cgacgtaacg cgttgcggca tccgcacgag cagaaacgct gcaagggtga gcgccggtct
     3901 cgggcaaaga gctcgtaaca ccccggccga tcgcatgcag aaacttccga attcgcacct
     3961 ggagggttac ccacgcgggg cgctggcccg cgaccggatt cgttcgatag ccgctggtcc
     4021 tgcccctgct tacgacggtc ccgtattcgt tcccttcgac ggcgagactg cgctttccgg
     4081 tgctgcttgc ccttctccgt ggtccggtat ttcttttgcg tgcgccatcg ccgcc
//
LOCUS       DAOSWW010000109         4122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14942_length_4122_cov_4.488812, whole genome shotgun sequence.
ACCESSION   DAOSWW010000109 DAOSWW010000000
VERSION     DAOSWW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14942_length_4122_cov_4.488812"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PK442_03355"
     CDS             complement(<1..863)
                     /locus_tag="PK442_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOO86715.1"
                     /translation="MNWNDHLPALLLMIPLFGAFAAPLVCLLGKTMRNVLLVGLSFVT
                     LLVALLLWKSVLAGGIAVYVMGGESFNLTLPSGMALPIRIIMEVDAFSAFMVVCGSIA
                     SFAGALFSTNYMEKFTGLGRFVSLYFLLTLGMLGMMITGDFFNFFIFIEIASIASFGL
                     VAFWRDRPEAIEASLKYTLVSQVGSMIFLIAAGALYGKYNALNIAAIGSLLQFGVMEK
                     IALVFIIGTLAMKCGSFPMHMWLPDAYAEAPSGVSCLLVAVSQASLYGLMRVCFSLYG
                     VNLGGAFIPWM"
     gene            complement(860..1207)
                     /locus_tag="PK442_03360"
     CDS             complement(860..1207)
                     /locus_tag="PK442_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOO86716.1"
                     /translation="MIGNAPFFVVGLLFLGGLTAIFIEKNLIKIAIAIGIISSAVNLF
                     LVALGYRAGGTAPIHFLAGERTVMVLPTPQAMTLTAIVISLATTAFLLSLIMLVYRHY
                     GTLDVDEIRRLRG"
     gene            complement(1197..1664)
                     /locus_tag="PK442_03365"
     CDS             complement(1197..1664)
                     /locus_tag="PK442_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOO86717.1"
                     /translation="MNPLSVVVRTGCDLFAWFMVVFGVNVLVHGYMTPGGGFQGGAII
                     ATFTAFLLVAYGGRKLLGWANEKIFDLFEEVGLLAFFFLAFMGFPVTFFYNYLVKPLA
                     AGGVVPPAGTTALMSIAVGLEVSGALSMVILTMYRGIRMFETAPMEEEIGHDR"
     gene            complement(1661..1924)
                     /locus_tag="PK442_03370"
     CDS             complement(1661..1924)
                     /locus_tag="PK442_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86718.1"
                     /translation="MIFVVAALLLGAMVVGAVDNAHPFGFPGKTPMDDFFLADALPNR
                     STENIVTAIVFDYRGFDTIGEAAVLFTALCAISVLFREGGRKR"
     gene            complement(1930..2190)
                     /locus_tag="PK442_03375"
     CDS             complement(1930..2190)
                     /locus_tag="PK442_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOO86719.1"
                     /translation="MTNALHISVLAVMLIAAFFALWFKDLISSVISLAVFSLMMALEF
                     YILQAPDVAIAEAAIGAALSTTIYIIALRACRRSKSEGGRKQ"
     gene            complement(2187..2561)
                     /gene="mnhG"
                     /locus_tag="PK442_03380"
     CDS             complement(2187..2561)
                     /gene="mnhG"
                     /locus_tag="PK442_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOO86720.1"
                     /translation="MVFFILVIAALLVSVFFNSLGAVSLYRFPDVYTRLHGATKCTTF
                     GTIFIAVAVVLYAVGRLLESGENRFWVLIIHTLVAMMVLLVTNATGAHAIARASHRSG
                     LAPAKAVVDRLEESCSRGGASQ"
     gene            complement(2567..2827)
                     /locus_tag="PK442_03385"
     CDS             complement(2567..2827)
                     /locus_tag="PK442_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOO86721.1"
                     /translation="MTVHFVWFVAAVMGILAVAVLGRLISGPTVSDRAVALDTFNTFV
                     VGIMLLLAVGYDSVVMVDVAIVYAALSFVGTMFIARYIEGEV"
     gene            complement(2824..3309)
                     /locus_tag="PK442_03390"
     CDS             complement(2824..3309)
                     /locus_tag="PK442_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOO86722.1"
                     /translation="MFIFFASFLMYLLLVWSGSGIPMIEVVIGLLMATVVTFAYKTWH
                     PEKKLSKRGLDPRRWFGFVAYLFGPFALGLAKANIDVAKRVITGKIRPGIVKVTPALK
                     SDVARTLLADSITLTPGTLTVDIDDAGAFYIHWLYVEDENPSEEQLYGNFAHWARRLA
                     E"
     gene            3643..>4122
                     /locus_tag="PK442_03395"
     CDS             3643..>4122
                     /locus_tag="PK442_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO86723.1"
                     /translation="MRLSPSLKTVIADISLVFVALFWGIGFVAMKDALDTFPTFWLLT
                     LRFGSAALLMGILFRRRLAAITKEDIRAGAIIGVFLFLGFATQTLGLNYTTPGKQAFL
                     TATYVVIVPFLSWGLRRIFPGALSFFASFICLVGMGMLTLQEGVLSIGKGDTLTLVCA
                     "
BASE COUNT         1046 a         1241 c         1073 g          762 t
ORIGIN      
        1 atcatccacg ggatgaaggc tcctccgagg ttgacgccgt acagcgagaa gcacacgcgc
       61 atcaggccgt agagcgacgc ctgactgacc gccaccagga ggcaggagac gcccgaaggc
      121 gcttccgcat aggcgtcggg aagccacatg tgcatgggga aggaaccgca cttcatcgca
      181 agcgttccga tgatgaaaac caacgcgatc ttctccatga cgccgaattg gagcaaactg
      241 cctatcgctg ctatgttgag cgcgttgtac ttgccgtaca gagcgcctgc ggcgatcagg
      301 aagatcatcg accccacctg cgagacaagc gtatatttaa gacttgcctc gatcgcctcc
      361 ggcctgtcac gccagaatgc gaccaaaccg aacgaagcga tggatgcgat ttcgatgaag
      421 atgaagaagt tgaaaaagtc gcccgtaatc atcattccga gcataccgag cgtaagaagg
      481 aaatagagcg agacaaaccg ccccaagccg gtgaacttct ccatgtagtt cgttgagaag
      541 agggcgccgg cgaacgaggc gatcgaaccg cagacgacca tgaaggcgct gaacgcgtcc
      601 acctccatga tgatgcggat cggaagcgcc ataccggagg gaagcgtcag gttgaagctc
      661 tcccctccca ttacatagac cgcgattccc cccgcgagca cgcttttcca gagaagaagc
      721 gcgacaagca gcgtcacgaa agaaagaccg accaacagca cattgcgcat cgtctttccc
      781 aaaagacaga ccaaaggcgc ggcgaaagcg ccgaagagag gaatcatcaa caacagcgcg
      841 ggaaggtgat cgttccagtt catcctctca acctcctgat ttcgtccacg tcaagcgtgc
      901 cgtaatgacg gtagaccagc atgatgagcg agagaagaaa ggccgtcgtc gcaagcgaaa
      961 tcacgatcgc ggtgagcgtc atcgcctggg gcgtcggaag caccatgacc gtccgctcgc
     1021 ctgcgagaaa atgaataggc gccgttcctc cggcacgata ccccaaagcg actaggaaca
     1081 ggttgacagc ggaactgatg ataccgattg cgatggcgat tttgatgaga ttcttttcta
     1141 taaagatcgc cgtcagtccg cccaggaaaa gaaggcctac cacaaaaaaa ggagcgttac
     1201 ctatcatgac cgatctcctc ctccatcggg gcggtctcga acatgcggat ccccctgtac
     1261 atcgtgagaa taaccatcga aagagcgccg ctcacttcga gtcccaccgc gatggacatg
     1321 agcgccgtcg tgcctgcggg cggaacgacg cctccggctg caaggggctt cacaagatag
     1381 ttgtagaaga acgtgacggg gaatcccatg aaggccagaa agaagaaagc aagcaacccg
     1441 acctcttcga aaaggtcgaa tatcttctcg ttcgcccagc ccagcagctt ccgcccgccg
     1501 taggccacca acaaaaaggc ggtgaacgtc gcgatgatcg cccctccctg gaagcctccg
     1561 ccgggagtca tgtacccgtg caccagaacg ttgacgccaa aaacgaccat aaaccatgcg
     1621 aacaggtcgc agcctgtccg gactacaacc gaaaggggat tcaccgtttc cttcctcctt
     1681 ccctgaaaag aaccgaaatt gcgcagagcg cggtgaataa aacagcggct tcgccgatag
     1741 tatcgaatcc tctgtaatcg aagactatcg ccgtgacgat gttctcggtc gatctattcg
     1801 gcagagcgtc cgcaaggaaa aaatcgtcca tcggagtttt ccccgggaag ccgaaaggat
     1861 gcgcattgtc cacggcgccg acgaccattg ctccgagcaa aagcgcagcg acaacgaaaa
     1921 tcatcttttt cactgtttcc gccccccttc actctttgaa cggcgacacg ccctgagcgc
     1981 gattatatag atcgtcgtac tcagagccgc tccgatggcc gcctcggcga tcgcgacgtc
     2041 gggcgcttgc agaatgtaga attccagcgc catcatcaga ctgaagaccg ccaaggaaat
     2101 caccgaagag atgagatcct tgaaccagag ggcgaagaac gcggcaatca gcataaccgc
     2161 cagaaccgag atgtgcagcg cgttcgtcat tgggacgcac ctcctctgga acagctctcc
     2221 tcaagcctgt cgacaacggc ctttgcaggc gccaatccgc tccggtgaga ggcccgcgcg
     2281 atggcgtgcg ctcccgttgc gttcgtgaca agcagcacca tcatcgccac aagcgtatgt
     2341 atgatgagca cccaaaaccg gttctccccg ctttcgagga gcctgccgac cgcatagagc
     2401 accaccgcga ccgctatgaa aatcgtaccg aacgtcgtgc acttcgtcgc gccgtgaagc
     2461 ctagtgtaca cgtcggggaa tcggtagagg gaaaccgcgc cgagagagtt gaaaaaaacg
     2521 ctcaccaaca gagcggcaat caccaaaata aaaaagacca tccgggttac acctcccctt
     2581 cgatatagcg ggcgatgaac atggtcccca cgaaggagag cgcggcgtac actatggcca
     2641 catcgaccat gacgaccgaa tcgtacccga cggcgagaag cagcatgatg cccactacga
     2701 aagtgttgaa cgtgtccaga gcaaccgccc tgtccgagac cgtcggaccg gagataagac
     2761 gcccgaggac ggcgacggcg agaatgccca taaccgccgc aacaaaccac acaaaatgga
     2821 ccgtcattca gccaacctcc ttgcccagtg ggcgaaattg ccgtacagct gttcttccga
     2881 gggattctcg tcctcgacgt acaaccagtg aatatagaag gctcctgcat catctatatc
     2941 cactgtgagc gttcccggcg tcagcgtgat ggaatcggcc agcaacgtcc ttgcgacgtc
     3001 gctcttcaac gccggcgtca ccttgactat gccgggacga atctttcccg tgatgactcg
     3061 tttcgcgaca tcgatattcg cttttgccag ccccaatgcg aacggtccga aaagatacgc
     3121 cacaaagccg aaccaccgcc tcggatcaag cccgcgcttg ctgagcttct tctccggatg
     3181 ccatgtcttg tacgcgaacg tgaccaccgt cgccataaga agacctatca cgacctcgat
     3241 catcggaata ccgctcccgg accagacaag gagaagatac atcaaaaacg aggcgaaaaa
     3301 aataaacaca tactccacct cccgtcacat agaattttga acacccgaat acacccccgc
     3361 ggaacaaaga aatgcatcga atacattata ctacctcttc ggtccttgta aatgaaagtt
     3421 aatactctca caaaatagaa acagcgggaa atggagcaga ggaaaatctc acactcgaaa
     3481 agtgcggtac aacaaccgcg gaagccccct gtttcacgca cacgccgact gaagccgcgc
     3541 aggcaaggct ctcaatgtat cttttttttc gcggaagatg ctatactctt ccggtattcg
     3601 tagtacggga ctcaataatt taccgtttcg ggagtgaatt cgatgcgcct ttctccatct
     3661 ctgaaaacag ttatagcgga catctcgctc gtcttcgtcg ccctcttctg gggcatcggc
     3721 ttcgtcgcga tgaaggacgc gctcgacacc tttcccacgt tctggctgct cacgctgcgc
     3781 ttcggctcgg cggcgctgct gatggggatc ctttttcgcc gccgcctcgc ggctattact
     3841 aaggaagaca tccgggcagg agcgatcatc ggggtattcc tcttcctcgg cttcgcgacg
     3901 cagacgctcg ggctgaacta caccacgccc ggcaaacagg cgttcctgac ggcgacctac
     3961 gtggtcatcg tcccctttct ctcgtggggg ctgcggagga tttttccggg agcgctctcc
     4021 ttcttcgcct ccttcatctg cctcgtcggc atgggaatgc tgacgctcca ggaaggtgtg
     4081 ctctccatcg gcaaggggga tacgctcact cttgtctgcg ct
//
LOCUS       DAOSWW010000110         4117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14965_length_4117_cov_5.552930, whole genome shotgun sequence.
ACCESSION   DAOSWW010000110 DAOSWW010000000
VERSION     DAOSWW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14965_length_4117_cov_5.552930"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PK442_03400"
     CDS             <1..425
                     /locus_tag="PK442_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOO86724.1"
                     /translation="LCGLGFISAMPGTLGSVAAFLFYVIFPVPWYLILVTCFLVVYVS
                     QIYSRRIGVEDPKEVIIDEVVGVWIAMYGMPSGFFLPALFLFRVVDILKPFPVNASEK
                     LPGGWGIMADDIVGGILVNLLLTFIRWLYIGGGWSHIL"
     gene            463..1680
                     /locus_tag="PK442_03405"
     CDS             463..1680
                     /locus_tag="PK442_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOO86725.1"
                     /translation="MKQAVLIALGDELLSGARREGNCGWLANRLALAGWKVLRMEIIP
                     DGLGALEDTLALWAGKTDLIVISGGLGPTHDDCTRKALARFLNAPLQVNAEAYNRILA
                     RYPSEMRENLELCRISQGSVPEGAQAIHNPRGSALGISFLMEGTKICAFPGVPDEFKA
                     MAEQELACEMSVSDCRMRSLIVVGWPESLLKDRIAPVVDDPSLHISILPSSGTVEIIL
                     RGEPVRIECAARTLRELLPGDCLPEGCFSVAEDLLREARKRNVTLAFAESCTGGLLGA
                     ALTDISGSSDVFLGSAVCYSNSAKTHILSVSDELLESFGAVSSECAIAMAKGALNQFG
                     SELALSVTGVAGPGGGSEEKPVGAVWFALAGLCGERAFLHHFPGDRLQIRRSAMNTGL
                     LLLRKTVMELNIL"
     gene            1677..2255
                     /gene="thpR"
                     /locus_tag="PK442_03410"
     CDS             1677..2255
                     /gene="thpR"
                     /locus_tag="PK442_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOO86726.1"
                     /translation="MNGILRSFFCIELPPAVQKKLWQTVDTLRLVLPDLKWVSANALH
                     ITLKFLGEQEQRLLEDLMRSFEKRIASSGQDVIALSSSALGAFPNIRTARTLFAELCG
                     DLVPLKAIASIVEDVSAEHGIPREKRPFKPHITLARTRRTETVIISPKDIPLFSKIEW
                     QATSVTLMKSSLTPAGPIYTPLKIWEFPAKVF"
     gene            2315..3460
                     /gene="recA"
                     /locus_tag="PK442_03415"
     CDS             2315..3460
                     /gene="recA"
                     /locus_tag="PK442_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOO86727.1"
                     /translation="MAKKKTPQTRDDVLELAIEDIRSKFGEGSIMRLGDSFKAAVEVI
                     STGILPLDVALGIGGLPRGRIVEIFGPEGSGKTTVALHAVAEAQKAGGVAAFIDAEHA
                     LDPRLAHNLGVDVQSLYIAQPDSGEQALYVLDTLVRSSAVDIVVIDSVAALTPQAEID
                     GKIGDSQVGLQARLMSYALRRLTSAISKSNCVVVFINQLRAKISTGYSQGPQETTTGG
                     RALKFYSSVRIEVKRGKGVSKGDDTIGHELWMKVVKNKQAPPFKTGHCTLLYGKGLPK
                     GMSILDMAIDFNVVKRKGSWLAYKGETLGQGKDTVAQYIEEHPDLMQEISAEVLAVTS
                     EGLSLALSEHESPLPLEDDENSVPSLSIEDGILELDEEVETGDEEEK"
     gene            3742..>4117
                     /locus_tag="PK442_03420"
     rRNA            3742..>4117
                     /locus_tag="PK442_03420"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1021 a          939 c         1106 g         1051 t
ORIGIN      
        1 ctctttgtgg attgggtttt atctccgcga tgcccggaac gctcgggtcg gttgctgcat
       61 ttctttttta cgtaattttc cctgttccgt ggtatttgat acttgttacc tgctttttag
      121 tagtctatgt gtcacagatc tattcgagaa gaataggcgt tgaggacccg aaggaagtaa
      181 ttatagatga agtcgttggt gtctggatag cgatgtatgg aatgccctcc ggttttttcc
      241 ttccggcgct ctttcttttc cgggttgtgg atatattgaa gccgtttcct gtgaacgcct
      301 cggaaaaact tcccggagga tggggaataa tggcggacga tatcgtcgga ggcattctcg
      361 tcaatctact cctgaccttt atccgctggc tctacatcgg aggcggctgg agtcatatcc
      421 tgtaaaaagg aacgacggct tttcggaaaa aggaggcata ccatgaaaca ggcagtcctc
      481 attgcccttg gtgatgaact tctcagcggt gcacgtcgcg aggggaattg cggatggctc
      541 gcgaatcgtc ttgctcttgc agggtggaaa gttctccgga tggaaatcat acccgacgga
      601 ctcggagcgc tggaggacac tctggctctt tgggctggaa aaacggacct tattgtcatc
      661 tcaggagggc tcgggccaac acatgatgat tgcacaagga aggcccttgc acggtttttg
      721 aacgccccgc ttcaggtgaa cgcggaagca tataacagaa ttttagcacg atatccttcc
      781 gagatgagag agaaccttga attatgcaga atttctcagg gctcagttcc tgaaggcgcc
      841 caggctatac ataacccgcg cggctccgct cttggtatat cctttctgat ggaagggaca
      901 aagatctgcg cctttccggg tgttcccgat gagttcaaag caatggctga gcaggaactt
      961 gcctgcgaga tgtctgtaag tgactgcagg atgcgttcac tcattgttgt cggatggccg
     1021 gagagtcttt tgaaagaccg aattgctcct gtggttgacg atccctccct ccatatttcc
     1081 attctcccct cttccggaac agtggaaatt attctcagag gagaacctgt tcgcatagaa
     1141 tgcgcggctc gcacgttacg agagctcctc cctggcgact gcctgccgga aggctgtttt
     1201 tccgtagcag aggatctcct tcgagaggcc cggaaaagaa acgtcacact tgcctttgcg
     1261 gaatcatgca ctggaggtct tcttggggct gcgctcacag acatctcagg gagttcggat
     1321 gtgtttttag gaagcgcagt ctgttacagt aacagcgcaa aaacacatat actctcagtt
     1381 tcagacgagc tgcttgagag ttttggggct gtaagttccg agtgcgcgat cgccatggcg
     1441 aagggtgctt tgaatcagtt tggctccgag ttggcgctct ctgtcacagg agtcgcggga
     1501 ccaggaggcg gctctgaaga aaaaccggta ggcgccgtct ggttcgctct tgcaggtctt
     1561 tgcggcgaac gtgcattttt gcatcatttc cccggggaca gactccagat acgtagaagt
     1621 gcgatgaata cagggctttt gctgctccgg aaaacggtaa tggagttgaa cattctatga
     1681 atggaatact gcgttctttc ttttgcatcg agttgcctcc tgcagtacaa aagaaacttt
     1741 ggcaaactgt ggacaccctt cgtttagttt tgccggattt gaaatgggta tcggcgaacg
     1801 ccctccacat aacgctcaag tttctcggcg aacaggagca acggctgctc gaggatctta
     1861 tgcgatcatt tgagaaaaga attgcttctt ccgggcaaga tgtgatagct ctttcctctt
     1921 ccgctctcgg tgctttccca aatatacgaa cggcccgaac tctttttgcc gaactgtgcg
     1981 gcgatcttgt cccgctgaaa gctattgcgt caattgttga agatgtgagt gcggaacatg
     2041 gaattccacg agaaaaaaga cctttcaagc ctcacataac acttgcgaga acgcgccgta
     2101 cggaaacagt gataatatcc ccaaaagata tccctctttt cagtaaaata gagtggcaag
     2161 cgacatcggt aacgttgatg aaaagttctc tgacacctgc cggcccaatc tatacccctc
     2221 taaaaatctg ggaattcccg gcgaaagttt tttgatagga acacgacttt tcctgtacaa
     2281 tactactgtt catattctgg aaggaggaat ttgtttggcg aagaagaaaa ctcctcagac
     2341 gcgtgacgac gttcttgaac ttgccattga ggatatccgc agcaaatttg gagaaggctc
     2401 cataatgcgt ctcggagatt cctttaaagc ggctgttgag gttatttcaa caggtattct
     2461 ccctttggat gtggctctgg gtatcggtgg ccttccccgt ggtcgtatcg ttgaaatttt
     2521 cgggcctgaa ggctccggga aaacaaccgt agctctgcat gccgttgctg aagcgcagaa
     2581 agccggagga gttgccgcct ttattgacgc tgaacatgct ctggacccga gacttgcgca
     2641 taatctcggc gttgacgtgc agtccttata tatcgcacag cccgacagcg gagaacaggc
     2701 tctttacgtt ttggacacgc tggttcgcag cagcgccgtt gatattgtgg ttatcgattc
     2761 agtcgctgca ctgacacctc aggcggaaat agacggtaaa attggagata gccaggtagg
     2821 ccttcaggct cgtcttatgt cctatgctct cagacgactg acctcagcaa tttcgaaaag
     2881 caactgcgtc gtggttttta taaaccagtt gcgcgcaaaa atcagcacag gctattcgca
     2941 ggggcctcaa gaaacgacaa ccggagggcg cgctctcaaa ttttatagct ctgtgcggat
     3001 agaagtaaaa cgggggaaag gcgttagtaa aggagacgac acaatcgggc atgaactatg
     3061 gatgaaggtt gtcaaaaaca agcaagcgcc acccttcaaa accggccact gtactctgct
     3121 ctatgggaag ggacttccta agggcatgtc gattctcgac atggccatcg actttaatgt
     3181 tgtaaaaaga aaaggctcat ggcttgctta taaaggagaa acattaggcc aggggaaaga
     3241 taccgttgcg cagtatattg aagaacatcc tgacctgatg caggagattt ccgcagaagt
     3301 acttgctgtt acctcagaag gactctcgct tgcactttcg gaacatgagt ctcctcttcc
     3361 tcttgaggat gatgaaaatt ccgttccttc tctttcaatc gaagacggaa ttcttgaact
     3421 cgacgaagag gtagaaaccg gagacgaaga agaaaaataa caataagggg aaaagcttta
     3481 taaataacca gcttgtgctt ttatagttag atactgtctt taaataatgc taatagcgat
     3541 tcttattctt cgcttattat ctctagcaga ttgcatgact cttcattgca attacaggat
     3601 gtttgcgttg acaacatctg gtacgtcagg tatcatactc tagcactttg gttgaggcca
     3661 agtgtttcgg gtgtgacccg agcagaacct tgacagtttt ataagagcga gaagaagagg
     3721 taaagaggcg ttccgagaaa tatattcgag agtttgatcc tggctcagga cgaacgctgg
     3781 cggcgtgctt aacacatgca agttgaacgg gcgatattgg aagcttgctg aagatatcgt
     3841 tagtagcgga cgggtgagta atgcatgagg acctgcctgc cggaggggga caacagttgg
     3901 aaacggctgc taataccccg tacgccgaga ggtgaaagga atggagacat tccgccggga
     3961 gagggactca tgtcctatca gctggttggt gaggtaacgg ctcaccaagg cgacgacggg
     4021 tagccggcct gagagggcga ccggccacac tggaactgag agacggtcca gactcctacg
     4081 ggaggcagca gtggggaata ttgggcaatg ggggcaa
//
LOCUS       DAOSWW010000111         4113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_14984_length_4113_cov_3.387137, whole genome shotgun sequence.
ACCESSION   DAOSWW010000111 DAOSWW010000000
VERSION     DAOSWW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14984_length_4113_cov_3.387137"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1048
                     /locus_tag="PK442_03425"
     CDS             230..1048
                     /locus_tag="PK442_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOO86728.1"
                     /translation="MVFCFMNGVFLDGDQATLPVSDLALQRGVAVFDTVRSYDGRPFA
                     LKAHLERLSNSARLARIAEPLSAEEIGDIVREGLSRIEGDGLAKIFLTGGDVEEFGSF
                     PSPRFLALFSPVAPIPEEFFTKGIALSLLQEERQLFRIKSINYMNAYRNHLPGTFEAL
                     HCVDGEITESATSSFFGVAKDNLITAPDHRVLRGITREILIDLARKEGYNVEYRCLTL
                     DELPDLTEAFITGSVKEVTPVTRIGDTIIGGGAPGAVTKRMYELFKESVPNHLG"
     gene            1109..1669
                     /locus_tag="PK442_03430"
     CDS             1109..1669
                     /locus_tag="PK442_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOO86729.1"
                     /translation="MGNGVYIVAAIAAGVLVYAVYIYNTLVRRKASVENGWSQIDVQL
                     KRRYDLIPNLVETVKGYAAHEREVFERIASLRSAALSATSPEQAAPLQNQLTGALRSL
                     FAVSENYPELKANQNFLALQEELASTENKIAFARQYYNDAVKAFNIAIDVFPNNLISR
                     RLGYEPRAMWAADDASELQSVQVTWR"
     gene            1674..2591
                     /gene="htpX"
                     /locus_tag="PK442_03435"
     CDS             1674..2591
                     /gene="htpX"
                     /locus_tag="PK442_03435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOO86730.1"
                     /translation="MVSFEGLIAHNKRLSILLFFLLLLLLGGLGASVSYWWGGEWYTG
                     VIVATAVAVIYFLFSWFAGASMILSMSGAREIRHEDAPQLWNVVEELSIAGGLPMPKV
                     YLIEDSAMNAFATGRDPEHASVAITRGLMEKLNREELQAVMAHEMSHVRNFDIRFAML
                     MAVMVGSIVMICDMVFRSVFFGRRRSSRSSDGGKGQAVMAVVALVLAILAPLLATIIQ
                     LAVSRQREYLADASAVELTRNPAGLVSALRKLSGDPNELQRASRGTQHLYIVNPLRKM
                     KQGKNSLFSTHPSLESRIERIEGLSLARA"
     gene            2588..2869
                     /locus_tag="PK442_03440"
     CDS             2588..2869
                     /locus_tag="PK442_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOO86731.1"
                     /translation="MTLIRKRFLVMGTVQGVGFRHFAARMALKFGVTGWVRNLPDGAV
                     EVHAQGEREAMDKMEQWLRKGPSSATVTRLVVLETPLKEGESSFAIRHH"
     gene            2910..4001
                     /locus_tag="PK442_03445"
     CDS             2910..4001
                     /locus_tag="PK442_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HOO86732.1"
                     /translation="MTAFLLLFSVGTAGYLVFKKFALPVPAILGSLFFVSVLNLAGFF
                     PEVQLQWISRYSNIVIGSYVGLRVNKQSLTLLRELPVPALIVSLSMLFLSLAGGSVLY
                     MMSDLSMKTAFLGSTTGGIAEMALLSMSMGADVASVTLLQVFRMLSAILVTPLVCRKW
                     TRWFGAGERSSCSSPSSLSVEPQFSDEEEAAALEPSVAPSAKGYALLAVVSLIGGILG
                     YIFRLPVGILTGAMFAVAATSLLRGEFPPMPNILRTVAQIGIGIIIGSNITISTLHQF
                     GELWLPVVALTAVLLVCSVLLAVLLHRMTGWDYPTCLLSSSLGGLSQMSIISEEMGAD
                     PLKVSILQTIRLLSILIVLPFLFSFLFGA"
BASE COUNT          777 a         1170 c         1236 g          930 t
ORIGIN      
        1 aggtcgtaac cctctcaagg ttaaaacacg ggttcgaatc ccgttggggc taccaaaata
       61 aaccgtacga atgtgcggcg ttatacattg aaagaccgct tgaacgagcg gtcttttttt
      121 attcttcttt actgaaataa ccgattcggc attgagcttc tcgcgtcctg atgttaggat
      181 agtactatca accgaacata ttttttacat aatcgaggtg ctttccaaca tggtattttg
      241 tttcatgaac ggggtgtttc tggatgggga ccaggcaaca cttcccgttt ccgaccttgc
      301 actgcagcgc ggagtggcgg ttttcgacac cgtccgaagc tacgacggcc gtcctttcgc
      361 tctcaaggcc catctggaaa ggctttcgaa ttcggcgcgg ctggcgcgga tcgcggagcc
      421 tctctctgcg gaagagatcg gggatatcgt ccgggagggg ttgtcgcgca tcgaagggga
      481 tggcctggcg aagatcttcc tgaccggcgg ggacgtcgag gagttcggtt cgtttccttc
      541 gccgcgattc ttagcgctct tctcaccggt cgctcctatt ccggaggagt tttttacgaa
      601 ggggatcgcg ctctcgcttc tgcaggagga gcgtcagctt ttcaggataa agagcatcaa
      661 ctacatgaac gcctacagga accaccttcc ggggacgttc gaggcgctcc actgcgtgga
      721 cggagagatt acggagtccg ctacgagcag cttcttcggc gtagcgaaag acaacctgat
      781 cacggctccg gaccatcgcg tgcttcgcgg cattacgcgg gagattctca tagatcttgc
      841 gcgaaaagag gggtacaacg tggagtaccg ctgcctcacg ctcgacgaac tgcccgatct
      901 gacggaggcc ttcatcaccg gaagcgtaaa agaggtgacg ccggtgacga gaataggcga
      961 cacgattatc ggcggcggcg ccccgggcgc ggttacgaag cgcatgtacg aactcttcaa
     1021 ggagagcgtt cccaaccatc tcggctgatt cgcgctggtg tataatgttt ttgttgaaga
     1081 acgtaatccg gaagggaggc tgtcgtccat gggaaatggc gtctatattg tcgctgcgat
     1141 tgctgcaggt gttctcgtgt acgccgtcta tatctacaac acgcttgtgc gcagaaaggc
     1201 gagcgtcgag aacggatgga gccagatcga cgtccagttg aagcggcggt acgaccttat
     1261 cccgaacctt gtggagacgg tgaaggggta cgccgcgcat gagcgggaag ttttcgagcg
     1321 catcgcctct ttacgaagcg ccgcgctttc cgcgacctct cccgaacagg ccgctccgtt
     1381 gcagaatcag ctcaccggcg cgctgcgttc actttttgcg gtctcggaga actaccccga
     1441 gctgaaggcg aatcagaact ttctcgcgct tcaggaggag ctggcctcca cggagaataa
     1501 aatcgcgttc gcccgccagt actacaacga cgcggtaaag gcgttcaaca tcgctatcga
     1561 cgtcttcccg aacaacctga tctcgcggag gctggggtac gagccgcgcg cgatgtgggc
     1621 ggcggacgac gcttccgagt tgcaatccgt tcaggtcacc tggaggtgag agcatggtca
     1681 gcttcgaggg gcttatcgcg cataacaagc gtctgagcat tttgctcttc ttcttgctgc
     1741 tgctgctgct cggcggtctg ggagcttccg tcagttactg gtggggcggc gaatggtaca
     1801 ccggggtgat tgtcgccacg gcggtcgcgg taatctattt cctgttctcg tggttcgcgg
     1861 gggcctccat gatcctctcg atgagcggcg cgagggagat ccgtcacgaa gacgccccgc
     1921 agctctggaa cgtcgtggag gagctctcca tcgcaggagg actcccgatg ccgaaggtct
     1981 atctgatcga ggacagcgcg atgaacgcct tcgccaccgg gcgggatccg gagcacgcga
     2041 gcgtggcgat cacgcgcggg ctgatggaaa agctgaaccg ggaggagctt caggctgtca
     2101 tggcgcacga aatgtcgcat gtgcggaact tcgacatccg cttcgccatg ctgatggcgg
     2161 tgatggtggg ctccatcgtc atgatctgcg atatggtttt ccgcagcgtc ttcttcggcc
     2221 gtcgccgcag cagccgttca agcgacggag gcaagggaca ggcggtgatg gcggtcgtgg
     2281 cgctcgtgct cgcaattctt gcgccgctct tggccacaat catccagctc gccgtcagcc
     2341 gtcagcggga gtacctcgcg gacgcctcgg cggtcgaact gacgcgcaat ccggcgggcc
     2401 tggtatccgc cttgagaaag ctgtccggcg acccgaacga actccagaga gcgagccgcg
     2461 gaacgcagca cttgtacatc gtcaacccgc tccggaaaat gaaacaggga aagaacagcc
     2521 ttttcagcac gcatccgtcc cttgaatcca gaatcgagcg catcgaaggg ctttcgctcg
     2581 cacgggcatg acattgatcc gcaagcgttt tctggtgatg ggaacggtac agggcgtagg
     2641 gttccgccac tttgcggcgc ggatggcgct gaagttcggc gtgaccggat gggtccggaa
     2701 tctgccggac ggcgcggtcg aagtgcacgc gcagggcgag cgtgaagcaa tggacaagat
     2761 ggaacagtgg ctccgcaagg gaccgtcttc tgcaaccgtg acgcgcctgg tcgtgctgga
     2821 aacgccgctg aaggaaggcg agagctcgtt cgctatccgt caccactaaa aaattcgcac
     2881 cgtgcggttc ggaggttcgg ttttctctta tgacagcttt tctcctgctt ttttcagtcg
     2941 gtaccgccgg ctatctggta ttcaaaaagt ttgctcttcc cgtaccggcg attctcggct
     3001 cgctcttttt tgtgagcgtc cttaatcttg ccggtttttt tcctgaagtg cagctgcaat
     3061 ggatctcccg atacagcaac atcgtcatcg gaagctacgt aggattgcgg gtgaacaaac
     3121 agtctctcac gctgctccgg gagcttcccg taccggcgct catcgtttcg ctgagcatgc
     3181 tcttcctctc cctcgcggga ggaagcgtcc tctatatgat gagcgatctc tccatgaaaa
     3241 cggcctttct tggatcgacg acgggaggaa tcgcggagat ggcgcttctc tccatgtcca
     3301 tgggggccga tgtcgcaagc gtgaccctgc ttcaggtctt ccgcatgctc tccgcgatcc
     3361 tggtcactcc cctggtctgc cggaaatgga ctcgatggtt cggcgccggg gagaggagca
     3421 gttgttcctc cccttcttct ctttccgttg aaccgcagtt ttcggatgaa gaggaagctg
     3481 ccgcgctcga accttctgtt gcgccatccg caaaggggta tgcgttgctg gcggtcgtgt
     3541 cgctcatagg cggcatcctc gggtacattt tcaggctgcc cgtgggcatt ttgaccggag
     3601 ccatgttcgc cgtagcggcg acgagtcttc tccgcggaga gtttccgccg atgccgaaca
     3661 tactccgaac ggtggcgcag ataggcatcg gcatcatcat cggctccaac atcacgatct
     3721 ccacgcttca tcagttcggc gagctctggc tgccggttgt cgcgctcaca gcggtcttgc
     3781 tcgtctgcag cgtcctgctt gccgtactgc tccacaggat gacgggatgg gactacccta
     3841 cctgtcttct ctcctcctcg ctcggggggc tctcgcagat gtccatcatc tccgaggaga
     3901 tgggggccga tccgctgaag gtgagcatac tccaaaccat ccgcctgctc agcattctca
     3961 tcgtgctgcc gttccttttt tcctttcttt tcggcgctta gccgcaggag cttcgtaccg
     4021 gcacgccgcg ctgtttcgtc atcttgcgga attgttcgac aaaaggccgg aagcgcgcgt
     4081 gcagctccat atcgggagag aacgtccgcg cgc
//
LOCUS       DAOSWW010000112         4110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15006_length_4110_cov_3.654007, whole genome shotgun sequence.
ACCESSION   DAOSWW010000112 DAOSWW010000000
VERSION     DAOSWW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15006_length_4110_cov_3.654007"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PK442_03450"
     CDS             <1..950
                     /locus_tag="PK442_03450"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO86733.1"
                     /translation="PEEAEEDSRRPPSSLVLRDMSPVLLSALEKGIPFHGTLEGAEEL
                     FSSMCGASACSRSSLDSVVIPVHLRGQFWGFLALCFRERHSAFEGDELGVLRSFACTL
                     AASVSRWESELEVSAGYENLQKTFSDVIRTMGQIVGKKDPYTIEHQERVGTLASAIGA
                     RMNLDAERCEGLRIAGLVHDVGKIEIAGEILNKPGRLSPIEFELVKTHSQSGYEILSG
                     IDFPWPVAEIALQHHERLNGSGYPRGLKGNEILLEARIIAVADVVESMISHRPYRPAL
                     GMDAAREELKARKGEFYDPEVVDACLGLLEERPDIIGAE"
     gene            complement(958..2253)
                     /locus_tag="PK442_03455"
     CDS             complement(958..2253)
                     /locus_tag="PK442_03455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO86734.1"
                     /translation="MRHASPSSRDEARGISSRRAHFLFLCLVGAYFFSFFFRISASVV
                     LPSQAAKLGMSAALTGFISSLYYYAYSVMQSISGALHDRFGPLRVVAGGMLVAAAGTF
                     LLVLHPSEWTLGAWRLLTGFGLAPMYGGALVYQASAFTPDKYVFYSSITISVGAFGAL
                     ASVAPLGLALDTFGLSATFAGLTLLSISMSVLLWKERSFDPVVHRTPFTEQKTQSILS
                     RLAEALRLIRSSAYLRSLLVLWTVSAGALLSFQGLWAVSWYQTAYGVAAATARNWASL
                     VSVGMFIGTAVFGGFCRRPEDRFRGVCFWTLFNVASWGALVSSVALKLPLPVAGTCGF
                     LIGAANGLIAVQASSAAKEQVETAKTGALLGTMNMIVIFVVVLFQWGTGILINLFPGG
                     QAGEFTERGFLVCFLIVVVLLAGSLPALPPLRRADTRRR"
     gene            2562..3257
                     /locus_tag="PK442_03460"
     CDS             2562..3257
                     /locus_tag="PK442_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOO86735.1"
                     /translation="MSVSSRIEDYMETIFAIEISGREATVTDLANTLGVAKATVVAAV
                     RRLVAASLATHERYGTLALTDEGRRRALDIYRRHEHLTFLFHDILGFDPDRAMSIACA
                     MEHELDEKSEGRILGFVDYLVKARREGRSWVKDLLTAMGDERRLPRPLSMLPDGGRGV
                     VTRVTAGGLLRQRLLEMGFVPGTEVCHCSTAPLGDPLVIHVRGCEVALRKTEAVSVWI
                     CPVTGEDSAGASC"
     gene            3280..3504
                     /locus_tag="PK442_03465"
     CDS             3280..3504
                     /locus_tag="PK442_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOO86736.1"
                     /translation="MCPITIMSEGTEVTVARVAGGGESSRRLSEMGLIPGAKVRIVQN
                     GGGPLLLKVGDSRFAIGQGMAHKVFVDGSC"
     gene            3587..3877
                     /locus_tag="PK442_03470"
     CDS             3587..3877
                     /locus_tag="PK442_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOO86737.1"
                     /translation="MGLTCSCSGGNERVLSALEIGEGGVVSKIAGDGSLKRRILEMGL
                     VPGTEIRVERKAPFNDPVSVWFRGYELSLRVDEANAVLVRPAGCAGCSGGSR"
     gene            3874..>4110
                     /locus_tag="PK442_03475"
     CDS             3874..>4110
                     /locus_tag="PK442_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOO86738.1"
                     /translation="MSWTIALAGNPNTGKTSLFNALTGSNQHVGNWPGVTVERKEGRF
                     RSSGDEVTVVDLPGIYSLGAASLDEEIAADYLLRN"
BASE COUNT          899 a         1050 c         1347 g          814 t
ORIGIN      
        1 ctcctgaaga ggccgaagaa gactcgcgca gaccgccttc atccctcgtt ttaagggaca
       61 tgtcgcccgt acttctctct gctctggaaa aaggcattcc gttccatggg actcttgaag
      121 gcgccgaaga gcttttttct tcgatgtgcg gcgcttccgc ctgctcgcgt tcttcgctcg
      181 acagcgtggt gatccctgtg cacctgcgcg gtcagttctg ggggttcctc gcgctgtgct
      241 tccgggaacg gcattccgct ttcgaaggtg atgaactcgg tgtgctgcgg agttttgcgt
      301 gcaccctggc cgcctcggtg agccgctggg agtccgaatt ggaggtgagc gccgggtacg
      361 agaacctgca gaagacgttt tcggatgtca tcaggacgat gggacagatt gttggaaaaa
      421 aagacccata cacaatagag caccaggagc gcgtcgggac gctggcttcg gcgatcggcg
      481 ccaggatgaa cctcgatgcg gagcgctgcg agggtcttcg tattgcaggt ctcgtgcatg
      541 acgtcggcaa gattgagata gccggtgaga tattgaacaa acccgggcgt ctttcaccta
      601 ttgagttcga gcttgtgaag acgcattccc aatcgggata tgaaatcctg agcggaatcg
      661 acttcccctg gcctgtagcc gagatcgcgc tgcaacatca tgaacggctg aacggctcgg
      721 gctatccccg gggactcaag gggaacgaga tccttctgga agcccgtatt atcgcggttg
      781 cggatgtcgt ggagtcgatg atttcgcacc gcccctaccg tcccgcgctg gggatggatg
      841 cggcgcggga ggaactcaag gcgcgaaagg gggagttcta cgaccccgaa gttgtcgacg
      901 cgtgtcttgg tcttcttgaa gagcggccgg atatcatcgg tgcagagtag aggcgctcta
      961 tcggcgtcgt gtgtcggctc gccggagcgg cgggagcgcg ggcagactgc cggccagcag
     1021 cactacaaca atcaggaagc agacgaggaa acctcgttcg gtaaactccc ccgcttgccc
     1081 tccgggaaag agattgatga gtattcccgt tccccactgg aagaggacca caacaaagat
     1141 gacgatcatg ttcatcgttc caagaagagc tcccgttttt gccgtttcca cctgttcttt
     1201 cgccgccgac gaggcctgga cggcaatgag cccgtttgct gcgcctatga gaaatccgca
     1261 tgtcccggcg acgggaagcg gcagtttcag cgctacggaa ctcactaacg caccccagga
     1321 tgccacgttg aagagagtcc agaaacacac cccccgaaaa cgatcctccg gacgccggca
     1381 aaacccgccg aaaacggcgg ttccaatgaa catgcccacg ctgacaagag aggcccagtt
     1441 gcgcgcggtt gcagccgcga ctccgtaggc ggtttgatac cacgagaccg cccagagtcc
     1501 ctgaaaagag aggagcgctc ccgccgagac ggtccagaga acgagcaacg acctgaggta
     1561 ggcggagctc ctgatgagcc ggagcgcttc ggccaggcgg gagagaatcg actgcgtctt
     1621 ttgctccgtg aagggcgtac ggtgcacgac aggatcgaag ctgcgctcct tccagagaag
     1681 cacgctcatg gaaatgctca aaagtgtgag ccctgcgaag gtcgccgaga gaccgaatgt
     1741 gtccagagcg agtccgagag gggcgacgga ggcgagggcg ccgaatgcgc cgacggagat
     1801 ggtgatgctt gagtagaaga cgtacttgtc cggggtgaac gcgcttgcct gataaacgag
     1861 cgcgccgccg tacatggggg ccagcccgaa accggtaagc agacgccatg cccccagcgt
     1921 ccattccgaa gggtggagca caaggagaaa cgtgcccgcc gcggccacca gcatcccccc
     1981 ggcgacgacg cggagcggcc cgaaacggtc gtgcagggcg ccggagatcg actgcataac
     2041 ggagtaggcg tagtagtaaa ggctggaaat aaagccggtg agagcggcgc tcatgccgag
     2101 ttttgccgct tgcgagggaa ggaccaccga tgcggagatc cggaagaaaa atgaaaagaa
     2161 atacgccccg acaaggcaga gaaataaaaa atgagcgcgg cgcgaggata tgccgcgggc
     2221 ttcgtcccgg gatgatggtg aggcgtggcg cataaaaatg agctccttcc tttgccgctc
     2281 ctttatcgcc gctgagaagc gtcgtgtgaa cttggcggtc ggcaagcgtt tcaagcggtt
     2341 gttctgtatc acggtgcatc ttacctcata aacggggaag agggtaggta tgaccgccgt
     2401 ttttaagagg ggctgaagaa gccggagagt gcgtttcgaa aaatataggt tgactgtcct
     2461 tgaatcgtag cgcttcatgc ttgacaggaa ttgaagcgcg aagtaagata agtctaacca
     2521 aaaagtaagt ctgctctcac ttctcgaaag gagggaatgc cgtgtcggtc tcatcccgga
     2581 ttgaggacta tatggagaca atcttcgcca tagagatttc cggacgggaa gctacggtta
     2641 ccgacctcgc gaatacgctc ggcgtggcca aagcaacagt tgttgcggcg gttcgcaggc
     2701 ttgtggcggc gtctcttgcg acgcacgaac gatacggtac tctcgcgttg acggatgagg
     2761 gcaggcggcg agcgctcgat atctataggc gccacgagca tcttaccttc ctttttcacg
     2821 atatccttgg cttcgacccg gaccgtgcga tgtccatagc atgtgcaatg gaacatgaat
     2881 tggacgaaaa gtcggaggga cggattctcg gatttgtcga ttaccttgta aaggcccgca
     2941 gagaggggcg ttcctgggta aaggaccttc tcacagcgat gggagatgag cgaaggcttc
     3001 caaggccgct ttcaatgctt cccgacggag gacggggtgt tgtgactcgc gtgactgcgg
     3061 gaggtttgct tcgccaacgc cttttggaaa tggggtttgt tcccggaacg gaagtgtgtc
     3121 actgcagtac ggcgccgctt ggcgatccgc ttgtgataca cgtacgcgga tgcgaagtgg
     3181 cgttgcgaaa gacggaggct gtctctgtat ggatctgtcc tgtaactgga gaggattcag
     3241 cgggagcttc ctgctgaacg aagagaggag atgggcagta tgtgtccgat aacgatcatg
     3301 tccgagggaa ctgaagtaac cgtagctcga gtggcgggcg gcggagagtc ttcgcggcgc
     3361 ctttccgaga tggggcttat ccccggagcg aaagtgcgga tagtccaaaa cggaggaggg
     3421 cctcttcttt taaaggtggg cgattcgcgt tttgcgatcg gtcaggggat ggcgcacaag
     3481 gtctttgtgg acggaagctg ttaggcggag cgctcaagga ccggcaggtc cttttttcag
     3541 attccgcgtt gagttagagt tagtgaacaa agaacggagg gtcattatgg ggttgacgtg
     3601 tagctgttcc gggggaaatg agcgtgtgtt gagcgctctt gagataggag agggcggcgt
     3661 cgtatccaaa atcgccggag acgggagctt gaagcggagg attcttgaaa tggggctggt
     3721 tccggggacg gagatacgcg tcgaaaggaa agcgcctttt aacgatcctg tctccgtgtg
     3781 gttcagaggc tatgaactga gtctcagagt cgacgaggcg aatgcggtcc tagtgcgccc
     3841 cgcaggctgc gcgggctgct cgggggggtc ccgatgagct ggacgatcgc gctcgcaggg
     3901 aacccgaata cgggaaagac gagtctcttc aatgcgctca ccggttccaa tcagcacgtg
     3961 gggaactggc ccggcgtgac tgtcgaacga aaagaggggc gattccgttc ctcgggagac
     4021 gaggtgactg tggtcgatct tcccggcatt tacagcctgg gggcggcgtc gctcgacgag
     4081 gagatcgcgg cggactatct gttgcggaac
//
LOCUS       DAOSWW010000113         4087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15122_length_4087_cov_3.841766, whole genome shotgun sequence.
ACCESSION   DAOSWW010000113 DAOSWW010000000
VERSION     DAOSWW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15122_length_4087_cov_3.841766"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PK442_03480"
     CDS             complement(<1..271)
                     /locus_tag="PK442_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOO86739.1"
                     /translation="MEHVFQVNDTPRPHREEIKVVGVGGGGGNALNHIIRSGVSGVHF
                     IAANTDVGALHLSQASSKVTLGEKLTKGLGAGANPEIGRAAAQEDR"
     gene            complement(304..1617)
                     /gene="ftsA"
                     /locus_tag="PK442_03485"
     CDS             complement(304..1617)
                     /gene="ftsA"
                     /locus_tag="PK442_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOO86740.1"
                     /translation="MYKEPEILVGLDLGTSKISVVIAERDSRFQEAQIIGVGYAPSQG
                     IRKGLIINLEQAVRSVRQAIKDAENMVGFEIDEATVSFSGMEVASVTSRGMISLGRTP
                     RQVSISDVERVIEAAQSELSIPPNRVTLHTIPVKYSIDGNSGIDDPLGMTGIRLEMEL
                     QSVIVPTTVVQNIINCVESAGIEVNGLVIKSLASALGSLTDEETRAGVISLCIGGGTT
                     GVALYTDGRPVKLSIIPIGGDHITNDLAYVMKIPISKAEEVKKMVSLNPEEIEQQDLD
                     VDVRGRMKTFSIGEAAEVVNCRLEELFGEHVRHQLAEFSLHMFPAGIVLSGGVAKTPG
                     IDVFVSETLNLPVRIAEPLDVYQMPPGRNDCEYTTVAGVVRYMTSRERNPYRYIETPI
                     SELRSGAAQVASSITGKLPRKSGGDRTEGIRGFMDYLKKSVKELF"
     gene            complement(1618..2412)
                     /locus_tag="PK442_03490"
     CDS             complement(1618..2412)
                     /locus_tag="PK442_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86741.1"
                     /translation="MGKWRGLFFLFAFFMGMLFGGEDFFQPFSLRNIRIESEDPVAEL
                     FFWKNISSRALRFWPAFFLEKDDLRKAVEIQAPLFCTISRRGITGVEVQIEPLRPWIV
                     AQWNKREYYVSQDGFAWDTANELNKMIRGIVRPNAPLITFSDDFPSPALENSSAIVSR
                     VVFPLELLQGWLDGLAENRWGARVEQISVSRREGKFLLKLVFDKGGAHVLLWGDRPRW
                     KELSSALSQIMEQLHFLGNNIIIDTTYTDRIIVRGMARGDQEGSGR"
     gene            complement(2505..3926)
                     /gene="murC"
                     /locus_tag="PK442_03495"
     CDS             complement(2505..3926)
                     /gene="murC"
                     /locus_tag="PK442_03495"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOO86742.1"
                     /translation="MDTTISNFDGIHRIHLMGIGGAGMSGLALLLAELGFEVSGCDMI
                     HTSYIDKVLKEEVAVVLGHGHAHMEKFLPDLLIYSSAIPEDNEEILAARARGIAVAKR
                     GEVLSWIFDSRCGIGVAGTHGKTTTSSMISLILEQAGFHPTLAIGGELCDIGVNAKLG
                     EGRFMVAELDESDGSFDKFHPEISVITNVDWDHVNFYPTFESVLEAFSRFFSNLKSDG
                     LLVFCGEDAGLARLLSLGRIPEGAISYGWGASWNWGASNVYHKPGGGVSFTLLHEGQR
                     VGEVELAISGEHNILNTLASCAVAHRLGIPFPVIKRTLRDFKGAKRRLQHVGSFEGID
                     VYDDYGHHPREIAATLGTLEHMFPERPLRVVFQPHRYTRTQALFKDFASVLAVADTLI
                     LLPIYQADEEPISGITSLLISDHLPAEKKQACLMPKTLDDAVFLLEDLCEEGDIVLTI
                     GAGDVSLIGDNLLAKAKTHLVRV"
BASE COUNT         1055 a         1092 c          908 g         1032 t
ORIGIN      
        1 cgcgatcttc ttgagcggcc gcgcggccga tttcggggtt ggcgccggcg cccagcccct
       61 tggttagttt ttctccaaga gtgaccttag aacttgcctg ggaaaggtgc aatgcaccga
      121 catccgtatt cgcagcgatg aagtgaaccc cggaaacacc gcttcgaatt atatgattca
      181 aggcgttgcc gccgccgccc cccacgccga ctactttgat ctcttctctg tggggcctcg
      241 gagtatcatt aacctgaaat acgtgctcca tattcatttc ctccccgaat gcggaatgcg
      301 gcttcaaaaa agctctttaa cagacttctt taaataatcc ataaaaccac gaatgccttc
      361 tgtccggtct cctccgctct ttcgagggag cttccccgta atgctggaag caacctgggc
      421 agctcctgag cgaagttctg aaataggcgt ttcgatgtat ctgtaaggat tgcgttccct
      481 gctcgtcata tatcgtacca caccggccac agttgtatat tcgcagtcgt ttcttcccgg
      541 cggcatctgg tacacgtcaa gcggttcggc aatccgaacg ggcaagttaa gtgtttcgga
      601 gacgaagaca tcaatgccgg gtgtttttgc tactcctcct gaaaggacga tgcctgcagg
      661 aaacatgtgg agactgaact cggcgagctg atggcgaacg tgttcaccaa aaagctcttc
      721 caatctgcag ttcactacct cggcggcttc accaatgctg aaagtcttca ttcgtccgcg
      781 aacgtcgacg tcaagatcct gttgctctat ttcctccgga ttgagagaga ccatcttttt
      841 aacctcttcc gccttactga tcggaatttt cataacgtac gcgaggtcgt tcgtaatatg
      901 atctccgcct atcgggataa tcgacagttt aacagggcgt ccgtcggtat agagggcgac
      961 tccggtagtt cctcctccga tgcacaacga tatcaccccg gcgcgtgtct cttcgtcggt
     1021 taaggatcca agagcactgg caagtgattt tattaccagt ccattgacct caataccagc
     1081 gctttcgacg cagttgatta tattttggac cacagttgtc ggaacaatta ccgattgcaa
     1141 ttccatttcg agacgaatgc ccgtcatacc tagaggatcg tctattccgg aattaccgtc
     1201 gattgagtat ttaacaggaa tggtgtgcaa tgtgacacga ttcgggggta ttgagagctc
     1261 gctctgcgct gcttcaataa cccgttccac atcggagata gagacctggc gaggcgttct
     1321 tccaagagaa atcatccctc ttgaagttac cgaagcaact tccatcccgc tgaaagaaac
     1381 ggttgcttcg tctatttcaa aaccgaccat gttctcagcg tcttttattg cttgacgtac
     1441 ggatcgcaca gcctgttcaa gatttatgat cagtcctttt cggatgcctt gggacggcgc
     1501 gtaccccacg ccaataattt gagcttcctg gaaacgggaa tccctctctg caataacgac
     1561 ggatatttta ctcgtaccaa gatccagccc tactaaaatt tccggctctt tgtacattta
     1621 tctcccgctc ccttcctgat cgccgcgcgc catgccccgg acgataattc tgtcggtata
     1681 tgtggtatct attataatgt tgttgccgag aaaatgtaac tgctccatta tctgcgaaag
     1741 cgcggatgaa agctctttcc aacggggacg gtctccccat aaaagaacgt gcgctcctcc
     1801 tttatcgaaa acgagtttta ataaaaactt tccttctctc ctcgaaacgc ttatttgctc
     1861 aactcgagcg ccccacctgt tttctgccaa gccatccaac caaccctgga gaagctccag
     1921 cgggaaaact actctggaaa caatagcaga agagttttcc aaagcaggag aagggaaatc
     1981 gtcagaaaaa gttatcaaag gtgcattggg acgcacaata cccctgatca ttttgttcag
     2041 ttcatttgcg gtgtcccaag caaaaccgtc ttgagaaaca taatactcgc gtttgttcca
     2101 ttgagctaca atccaaggtc gtagcggctc tatctgaact tcaactcccg tgattcccct
     2161 tcggcttatc gtacaaaaga gaggagcctg tatctcgact gcctttcgca aatcatcttt
     2221 ttccaagaaa aacgcaggcc agaatcgcaa cgcgcgcgaa ctgatattct tccagaaaaa
     2281 caactccgct acggggtcct cagattcaat acgaatatta cgtaaagaaa acggttgaaa
     2341 aaagtcttct cccccgaaga gcattcccat aaagaaggcg aaaagaaaaa ataaaccgcg
     2401 ccatttaccc attatacatc acaaaaatgg ctcgagtaaa aagcaggaaa gcaataagaa
     2461 aagaattcac ttttcctcaa acccctgaac gctggtttgg aaaattacac acgcactaaa
     2521 tgtgtctttg cctttgcaag caaattgtct cctattaacg acacgtctcc tgcaccaatg
     2581 gtcaagacga tgtctccttc ctcgcaaagg tcttccaaaa ggaaaacagc atcatcaaga
     2641 gtttttggca tcaggcacgc ttgctttttc tcggccggta gatggtcgga aattaggagc
     2701 gacgttatcc cagagatcgg ttcttcgtca gcctggtaga tcgggagaag aatcagtgtg
     2761 tctgcgactg caagaacaga tgcgaagtct ttaaacaaag cctgggtcct cgtgtatctg
     2821 tgcggctgga agacaacccg aagcggcctt tcagggaaca tatgttccag cgttcccaag
     2881 gtggctgcga tctctcgcgg gtgatggcca tagtcgtcat agacgtcgat accctcaaaa
     2941 cttccgacat gctggagacg gcgtttcgcc cctttaaagt cccgcagggt tcttttaatg
     3001 acaggaaacg ggattccgag acgatgtgca accgcacagg aagcgagcgt gtttaatatg
     3061 ttatgttctc cagatattgc cagctcaacc tcgcctactc gttgtccctc gtgcagaagc
     3121 gtgaaactga cgccgccgcc cggtttgtga tagacattgc ttgcccccca attccaagaa
     3181 gcgccccagc cgtacgatat ggctccctca ggaatacggc caagggacag caagcgcgca
     3241 aggcccgcat cctcaccgca gaaaacaagg agaccgtctg atttgagatt ggagaaaaaa
     3301 cgtgaaaaag cttcaagaac actttcaaac gtagggtaaa aattgacgtg atcccagtca
     3361 acgtttgtaa tgacggaaat ttcgggatga aatttgtcaa aagaaccgtc gctttcatcg
     3421 agttcggcaa ccataaagcg tccttctcca agttttgcgt tgacgccgat atcacacaac
     3481 tctcccccga tagccagggt gggatgaaaa ccggcctgtt ccaaaataag cgagatcatt
     3541 gaagacgttg tggttttccc atgagttccg gcaaccccta ttccgcaacg cgaatcaaaa
     3601 atccagctca gtacttctcc cctttttgct acagcgatcc cgcgtgctct ggcagcgagg
     3661 atttcttcat tgtcttccgg gatggcgctg ctgtatatta aaagatccgg aagaaacttt
     3721 tccatatgag catgtccgtg tcccagaacc actgccactt cttccttgag taccttgtcg
     3781 atataagaag tatggatcat gtcgcagccg ctcacttcaa aacccaattc agccagcaaa
     3841 agggccaatc cgctcatacc tgcgccgccg atgcccatga gatgaattct gtgaatgcca
     3901 tcaaaattcg atatagtcgt atccacttat aaaatctccc ttccaatact ggacgagatc
     3961 agccgccata aagattcaga ctcatctccc tccgaaagac accgtgctgt tctctgtgta
     4021 ttccgaagaa ttttcagtat gcattcttgc aacgctttcg gggaatcatt ctcctgccag
     4081 atcgcgc
//
LOCUS       DAOSWW010000114         4085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15140_length_4085_cov_3.133499, whole genome shotgun sequence.
ACCESSION   DAOSWW010000114 DAOSWW010000000
VERSION     DAOSWW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15140_length_4085_cov_3.133499"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK442_03500"
     CDS             complement(<1..182)
                     /locus_tag="PK442_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOO86743.1"
                     /translation="MTSFCIVLSGRGSNMEALADAVRDGRLDARVEFVASDAPDAPGL
                     EKAHRRGIDTVVLPYAS"
     gene            complement(179..1177)
                     /gene="purM"
                     /locus_tag="PK442_03505"
     CDS             complement(179..1177)
                     /gene="purM"
                     /locus_tag="PK442_03505"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOO86744.1"
                     /translation="MNLSYKNAGVDISLADKWVDVIKGISKSMPAKKEIIGGIGGFSG
                     LYRLPGGMALAGCCDGVGTKIEIARAAGMFTHIGQDLVAMNVNDLITCGARPLFFLDY
                     IACGKLNIPVLQPIVESVARACGECGCALLGGETAEMPGVYPEDGLDLAGFAVGIVEE
                     EKIIDGKNVAAGDAVVGIPSSGVHSNGFSLVRRALFSEGTPYSLDEAMPRFGGATLAE
                     ILLAPTKLYVNTALRAVSTGKVKAMAHITGGGLADNLRRVVPDGLGLSLDYTAWPRPA
                     IFNLLSEEGVEEEEMRRVFNLGIGYCLIIAQEDVSLVLEQIEAQREQGIIIGEVVA"
     gene            complement(1161..2534)
                     /gene="purF"
                     /locus_tag="PK442_03510"
     CDS             complement(1161..2534)
                     /gene="purF"
                     /locus_tag="PK442_03510"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOO86745.1"
                     /translation="MCGVFGAFSPDGKKVLEDVYLGLYALQHRGQEAAGVAWTNATGG
                     ISSRKGNGLVHLALDQADLAAVESSCAIGHVRYSTTGGSELSNAQPLTASTSKCPIAV
                     AHNGNLTNAQGLKLLLQNRGAIFHSTTDTEVILHLIAHQPHKQPLDALLDSLVRLRGA
                     YSLAMILGNRLVAARDPWGFRPLVLGRRDNIHYVSSETCALNLVGASVVREIEPGEVI
                     IIGEDGVHSIRLPVEPKRRFGCAFEFVYFARPDSVISGRSVYAVRKELGKKLAKRAPC
                     REASLVTGMPDSGTIASLGYAEESGVPYEQSVVRNRYVGRTFIEPTSRVRRLGVRIKL
                     NPITEVLQGKNVVVVDDSIVRGTTCERMISMIKSCGANQVHVRISSPPVRFPCYYGID
                     TPTRVELAAARMNLQQLEQEVGADSLAYITEEDLLEAIGLPAQSVCTACFSGDYMEGG
                     EEYEPQL"
     gene            complement(2535..>4085)
                     /locus_tag="PK442_03515"
     CDS             complement(2535..>4085)
                     /locus_tag="PK442_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOO86746.1"
                     /translation="SKTGRDGIAGAAFASVELSEDNKASRPSIQIGDPFGEKLLIEAC
                     LEMNEKKLIVSMQDMGAAGIISSSSEVAAKSGVGMILHFDKIPLRAENMEPWEIALSE
                     SQERMLLIVDRSYLHEVMEVAEKWELDCAEFGETVEGDEYTILFHGERAASLPATLIG
                     DRCPLIPWPSRRPADLEKKWEFDVDDIPLPEDWNETLLSLLADPSLASKKRIYEQYDS
                     MVQLNTVIGPGHPVSMLRIKGRDSLIAMTLDADPWKCSLDPFRGGAETVARTVRDLAV
                     AGARAAGLTDCLNFPSPEVPEQYWVLEECVKGMAEACGAFDCPVVSGNVSLYNESPDG
                     AILPTPVVGTVGIIPSIDAALSAGTWEEGDVLFLVGPCNPSLAGSRYLLSQGKLAGRP
                     LPFYPEAEKDFVERALKTASAKAAHSGRALSGGGLAAALAKESMESGIGASISLSIPT
                     RKDILLFGEGCARALYAVPMSRVPLFKVLWNGYPCIQLGRVGGERLSVEGAFSLTVSE
                     LLSKWRNG"
BASE COUNT          873 a         1236 c         1125 g          851 t
ORIGIN      
        1 gaggcgtacg gaagaacaac cgtgtcgatt ccccgtctgt gcgccttctc aagtccgggg
       61 gcgtcgggcg cgtcggatgc aacaaattcg accctcgcat caagccttcc gtcgcgcacc
      121 gcgtcggcaa gcgcctccat gttgctcccc cgtcctgaga ggacgatgca gaaggacgtc
      181 atgcaacaac ctctccgata atgattcctt gctctctctg cgcttcaatc tgctctagaa
      241 ccagcgacac atcttcttgc gctatgatca ggcagtagcc aataccgagg ttgaagacgc
      301 ggcgcatctc ctcctcttcg accccctctt cggagagcag gttaaaaata gcgggcctcg
      361 gccaggctgt atagtccagg gagaggccga gtccgtccgg aacgacgcgc cgaaggttat
      421 ctgcaagccc gccgccggta atgtgcgcca tagccttcac ttttccagtg gagacggccc
      481 gaagggctgt gttgacgtag agctttgtcg gagcaaggag aatttccgca agcgtggcgc
      541 ctccgaagcg cggcatcgct tcgtcaaggc tgtacggcgt accttcagaa aagagagcgc
      601 ggcgaaccag ggaaaaaccg ttgctgtgaa cgccggagct gggaataccg acgacggcgt
      661 ctcccgccgc aacatttttc ccgtcgatta tcttttcttc ttcgacgatg ccgaccgcaa
      721 acccggcgag gtcgagtcca tcctcggggt agacgcccgg catctcggcg gtttcaccgc
      781 caagcagtgc gcacccgcac tcgccgcacg ccctggcgac actttcgacg atcggttgaa
      841 gaacggggat attcagtttc ccgcaggcga tgtagtcaag aaagaagagc ggacgggctc
      901 cgcaggtgat cagatcgttg acgttcatgg cgacaagatc ctgcccgata tgggtaaaca
      961 ttcccgcggc cctggcaatt tcgatttttg tgcctacgcc gtcgcaacag ccggctagag
     1021 ccattcctcc agggagtctg tacagaccgc tgaaccctcc tatgcctccg atgatctcct
     1081 tctttgcagg cattgatttg gaaattccct tgataacgtc gacccactta tccgcgaggg
     1141 agatatcgac tcccgcgttt ttatagctga ggttcatatt cttcgccgcc ttccatgtaa
     1201 tctcccgaaa aacaggctgt gcagacgctc tgcgcgggaa ggccaatagc ttccagaaga
     1261 tcctcttcgg tgatgtacgc cagcgaatcg gcgccgacct cctgctcaag ctgttgaaga
     1321 ttcatgcgcg cggctgccag ttccacccgc gtgggggtgt cgatcccata gtagcaaggg
     1381 aagcgcaccg gaggagagga gatacgtaca tggacctggt tcgcgccgca cgacttgatc
     1441 atggagatca tccgttcgca tgtcgtcccg cgcactatcg agtcgtcgac gacgacgacg
     1501 ttcttccctt ggagcacctc ggtgatggga ttcagtttaa tccgcactcc gaggcgccgc
     1561 accctgcttg tcggttcgat gaaggtccgt cccacgtacc ggttgcggac aacgctttgc
     1621 tcgtagggta cgccgctctc ttccgcgtag ccgagagaag cgatcgttcc gctgtcgggc
     1681 attccggtga ccagagaggc ttcccggcaa ggcgctcttt tcgcaagctt tttccccagt
     1741 tccttacgta cggcatagac cgaacggccg ctgatcacgc tgtccggacg cgcgaaatag
     1801 acaaactcaa acgcgcagcc gaatcgtctc ttgggctcga caggaaggcg tatcgaatgt
     1861 accccgtctt ctcctattat gataacctct ccaggttcaa tctcccgcac gacgctcgct
     1921 cccacaagat tgagcgcgca cgtctccgaa gaaacatagt gaatgttatc ccggcggcct
     1981 agtaccagcg ggcgaaagcc ccaagggtcg cgagcggcta ccagccggtt tcccaggatc
     2041 atcgcgagag agtacgctcc ccggagacgt acaagagaat ccagcagcgc gtccagaggc
     2101 tgcttgtggg gttgatgggc gatgagatgg agaatcactt ccgtatccgt cgtcgaatga
     2161 aagatggcgc cgcggttttg aagaagtagc ttgagtccct gcgcattggt cagatttccg
     2221 ttatgcgcca cggcgattgg gcacttcgat gtgctcgcgg tcaggggttg cgcgtttgaa
     2281 agctccgatc ctccggttgt ggagtaacgg acgtgcccga ttgcgcacga agattccaca
     2341 gcggcaaggt ccgcctggtc gagcgcaaga tgaacaagtc cgtttccctt acgggaggat
     2401 attccgcccg tagcgttcgt ccaggcaacc cccgcggctt cctgccccct gtgctgaagc
     2461 gcgtaaagcc caaggtacac atcttccaga actttttttc catcgggtga gaatgcgccg
     2521 aagacgccgc acattcaacc attcctccat ttcgagagaa gctccgaaac agtaagcgaa
     2581 aaagctccct cgacggagag gcgctctccc cctacacgtc cgagttgaat gcaagggtac
     2641 ccgttccaaa gcactttaaa aagaggaacc ctgctcattg gaaccgcata aagcgcgcgt
     2701 gcgcagcctt cgccgaagag caaaatatct ttgcgcgtag ggatggaaag agaaatagaa
     2761 gcgccgatac cgctctccat gctctctttg gcaagggctg ccgcaagccc ccctcccgaa
     2821 agggcgcgcc cgctgtgtgc ggcttttgcg gacgcagtct tcagggctct ctcaacaaag
     2881 tctttttcag cttccggata gaaggggagc gggcgtcctg caagctttcc ttgtgagaga
     2941 aggtagcgac tcccggcgag cgatggattg cacgggccga cgaggaagag aacatcgccc
     3001 tcttcccacg taccggccga aagcgcagca tcgatcgacg gaataatccc gactgttcca
     3061 accacgggag tcgggaggat ggcgccgtcg gggctttcgt tgtataaact cacatttccc
     3121 gagaccacag gacagtcaaa ggcgccgcac gcctcggcca tccccttgac gcactcttcg
     3181 agcacccagt actgctccgg aacctccggg gaaggaaagt tcagacagtc ggtaagccct
     3241 gcggcgcgtg cgccggcgac tgcgagatca cggacggttc tcgcgacagt ttcggccccg
     3301 ccgcggaaag gatcaagcga acacttccag ggatcagcgt caagagtcat tgcaatgagc
     3361 gaatcccgcc ccttaatcct gagcatgctc acaggatgcc ccgggccgat aacggtgttc
     3421 agctggacca tcgaatcgta ctgctcgtag atccttttct tcgaagcgag cgaaggatct
     3481 gctagaagag agagcaacgt ttcgttccag tcttcgggaa gaggaatatc atcgacgtcg
     3541 aattcccatt tcttttcaag atccgcaggg cgtcgggaag gccagggaat aagagggcac
     3601 cggtctccga tgagcgtcgc agggagtgaa gcggctctct ccccgtggaa gagaatcgtg
     3661 tactcgtcac cttcgactgt ctcgccgaat tcggcgcagt ccagctccca cttttccgca
     3721 acctccatca cctcatgcag ataagaacgg tcaacgatca gcaacatgcg ctcctgcgat
     3781 tcggagagag cgatctccca cggttccata ttctccgccc tgagaggaat cttatcaaag
     3841 tgcaaaatca tcccgacgcc gcttttcgcc gcgacttcgc ttgaagagga gataatcccc
     3901 gccgcaccca tgtcctgcat actcacgatt agttttttct cattcatttc gaggcacgcc
     3961 tcgatcagga gcttttctcc gaacggatcg cctatctgga tcgatggtct gctcgccttg
     4021 ttatcctcgg agagctctac cgaggcgaac gcagctccgg cgatcccatc ccttcccgtc
     4081 ttcga
//
LOCUS       DAOSWW010000115         4083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15147_length_4083_cov_3.662115, whole genome shotgun sequence.
ACCESSION   DAOSWW010000115 DAOSWW010000000
VERSION     DAOSWW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15147_length_4083_cov_3.662115"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK442_03520"
     CDS             complement(<1..888)
                     /locus_tag="PK442_03520"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOO86747.1"
                     /translation="MKKILCFFLAAAGLFMLIRTTAFSPERWPFPLPSGSGELLPVMV
                     QTGMNARQAADEFVAAGIVEKGRAASFARWMVRFSIDRRLRPGVYHVRKGSPWEVAMQ
                     MKTAEPGFLSLTLVPGTDIYSFGTLFPDKSLSGESLEKLLADESLYPSEMRNLLPETT
                     EGRLAFLLPETYHLAFLNERELVSAAAALWWRRIGSSIPPEQRSDEVFEELAILASLV
                     EREGFRDDERPRIAGVLSNRMEKGMQLQIDATVVYAWRRKGRDITRVLFKDLEIDSPY
                     NTYKIQGLPPAPICTSSEIS"
     gene            complement(914..1930)
                     /gene="amrS"
                     /locus_tag="PK442_03525"
     CDS             complement(914..1930)
                     /gene="amrS"
                     /locus_tag="PK442_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOO86748.1"
                     /translation="MKNAEGSKETVREAEWWRFEGEDVRCMLCPHVCLISPGKAGRCG
                     VRIHVKGEGLRLSNYGMAASLAADPIEKKPLYHWKPGTSVLSLGSVGCSMHCPFCQNW
                     QLAECSASVSLQRIAPEDPARLAMQHGTPSVAFTYNEPLTWFEFLKDSCRLLREEKIP
                     VVLVSNGMINAAPLEEIVPYIAAANIDLKAFTPDAYRVMGGELDAVKRTIRTLSAAGT
                     HVEVTFLLVPGINDDAFSFAEMASWLASLHPVPVLHISRYFPNREWRVPATPLERIKE
                     FERIAHERLPWVYPGNIDGESCTRCLECGRVLLRRRRYTLLEKNIDEKGRCAFCESPT
                     PVTL"
     gene            complement(1927..3249)
                     /gene="amrA"
                     /locus_tag="PK442_03530"
     CDS             complement(1927..3249)
                     /gene="amrA"
                     /locus_tag="PK442_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOO86749.1"
                     /translation="MWYWECFTPHPPVIVPEVGRGRERDAFQTLEGMNAVSSLASTNP
                     PDTILLLSPHAPFSGGLTISLAEQYSGNFAQFAAPRTRLSLRGASEEGEGLARTLEKS
                     FPVVASRRKECPLDHGSLVPLFFLQKSGIPLPSLVVANPIGLSPSEAFRLGQALLKAP
                     LHGSWALVASGDLSHRVTPDAPAGYTPFGREFDRMVVEAFLTNDPAQLLALGEREMDE
                     AGECGLRSALLFLGLGEKRQIRSLSYEAPFGVGYATAFASLHSAPLLARTVLSEGVRS
                     GREAAKRAAASFHEFPELQKRSACFVSLKIRPGRTEDEQLRGCIGTILPSSSSLHEEI
                     VENTLAAAQRDPRFMPVTPEELENISLSVDILSTPEPVSSLDMLDPRKYGVLVEKNGL
                     RGVLLPDLEGVDTVEQQLSIAARKAGILSLERASLSRFTVRRLKEDAE"
     gene            complement(3290..>4083)
                     /gene="queA"
                     /locus_tag="PK442_03535"
     CDS             complement(3290..>4083)
                     /gene="queA"
                     /locus_tag="PK442_03535"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOO86750.1"
                     /translation="LLKALNPEWSEWEALVKPGRRLRSGTGIILSDGTAITVGERFEG
                     TEGVRRVSFPQGVKVLSLLTEIGETPLPPYITRSTAPRSAYQTVFAARDGSAAAPTAS
                     LHFTEELLEELRREIGLSVAWVTLHVGLGTFRPVQCSDIRKHVIHEEYCVLPLETKKL
                     VEETKRRGGRVIAAGTTVARTLESMAAEDGEMRWGEMNTQLFIYPGFEYKVVDALVTN
                     FHLPKSSLLMLVAAFAGYEPVMKAYETAVSMKYRFFSFGDAMFIC"
BASE COUNT          991 a         1192 c         1075 g          825 t
ORIGIN      
        1 ggagatctcg gaggaggtgc atatcggtgc aggaggaaga ccttgtattt tatacgtatt
       61 atagggagaa tctatttcca ggtccttaaa gagcacgcgc gttatatcgc gtccctttct
      121 tctccaagca tagacgactg ttgcgtcgat ttggagctgc atccccttct ccattctatt
      181 ggaaagtacg ccggcgatgc gcggacgttc gtcgtcccgg aatccttcac gttctacaag
      241 ggaagcgagg atcgccaatt cctcaaaaac ctcgtcgctc ctctgctccg gtggtattga
      301 ggaacctatt cgtctccacc agagcgcggc agccgcagag accagctctc tttcattcaa
      361 gaaagcgagg tgatacgttt cgggaaggag aaaggcgagc cggccttcag ttgtttcggg
      421 gagcaaattg cgcatttccg atggatacag gctttcatcc gccaacagtt tttccagcga
      481 ctctccggaa agtgacttgt ccgggaagag cgtgccgaaa gaatagatat cggtaccggg
      541 tacaagcgtc aaagaaagaa agcccggctc tgcagttttc atttgcatcg ctacttccca
      601 aggagagccc ttccgaacat ggtacactcc gggccgcaga cgacggtcga tggaaaagcg
      661 caccatccag cgagcaaatg aggcggcccg gcccttctca acaatgcccg ccgccacaaa
      721 ctcatccgct gcctgccgcg cgttcatccc cgtctggacc atcaccggaa gcagttcgcc
      781 gcttccgctc gggagaggaa acggccaccg ttctggagag aacgcagtgg tacggatcaa
      841 catgaagagt ccggcagcag cgaggaaaaa acacagtatt tttttcatag aaatgcattc
      901 ctcctagaga cagctaaaga gtgaccggcg tcggcgactc gcagaaagcg caccgcccct
      961 tttcgtcaat attcttttca agaagagtat agcggcgtct tcgaagcaaa actcggccgc
     1021 actccaaaca acgcgtacaa ctctctccgt cgatatttcc gggatacacc cacggaagtc
     1081 gttcatgtgc aatacgctcg aactccttga tacgttccaa aggagtcgcg ggaacccgcc
     1141 attctcgatt cgggaagtag cgggaaatat ggagcacagg aaccgggtga agagaagcga
     1201 gccaagacgc catctccgca aaggagaaag cgtcatcgtt gattccaggc acaaggagaa
     1261 aagtgacttc tacgtgcgtt cccgcggccg aaagagttct gatcgtcctt tttaccgcgt
     1321 cgagctctcc ccccataaca cggtacgcat ccggcgtaaa ggctttcaag tcaatgtttg
     1381 cggcggcaat gtacggaacg atctcctcca atggagctgc gttgatcatt ccgttgctta
     1441 ccagcacgac ggggatcttt tcctctcgaa ggagacggca ggaatccttg agaaactcga
     1501 accaggtcaa aggctcattg taggtgaacg cgacggaggg cgttccgtgc tgcatcgcga
     1561 ggcgcgcggg atcctcaggg gctatcctct gcaatgaaac cgacgctgaa cactccgcaa
     1621 gctgccagtt ctgacagaag ggacagtgca tcgaacaccc tacggatccc agggacagta
     1681 ccgaagttcc cggcttccag tgatagagag gttttttttc gatgggatcc gctgcgaggg
     1741 aggccgccat gccgtaattc gagaggcgaa gaccttctcc cttcacgtgg atacgcacgc
     1801 cgcaacgccc cgccttcccc ggagaaatga gacacacatg aggacagagc atacaccgta
     1861 catcttctcc ttcgaagcgc caccactcag cctcacgcac tgtttctttc gaaccctccg
     1921 cgttcttcac tccgcatcct ccttgagcct gcgaacagta aaacgagaga gagacgccct
     1981 ctccagagag agtattcccg ctttccgggc agctattgaa agctgctgtt cgacggtatc
     2041 tactccctcc agatcaggga gcaagacccc tcgcaaaccg ttcttttcga cgagcacacc
     2101 gtatttacgc ggatcgagca tgtcaagaga ggacaccggc tccggagttg agagtatgtc
     2161 gactgaaaga gaaatgttct cgagctcttc cggggttaca ggcatgaaac gagggtcgcg
     2221 ctgcgccgcg gcaagcgtgt tctccactat ctcttcatga agcgaagaag aggaaggaag
     2281 aatcgttccg atacacccgc gcagctgttc atcctctgtt ctccccgggc gaatcttcag
     2341 gctgacgaaa caggcggatc tcttctgaag ctccgggaat tcatgaaaac tcgccgccgc
     2401 tctttttgca gcctctcgtc cggaacgcac accttcggag aggactgtac gcgccagcaa
     2461 cggcgcgctg tgcaacgatg caaaggcagt cgcgtaccca acgccgaagg gagcctcgta
     2521 tgagagactc cgtatttgcc gtttctcgcc caacccgagg aaaaggagcg ctgaacgcaa
     2581 accgcattct ccggcctcgt ccatttcgcg ttctccaaga gcgaggagct gtgcgggatc
     2641 gttcgttaga aaagcttcga caaccatcct gtcgaactcg cgcccgaaag gggtgtagcc
     2701 ggcgggagca tcaggggtaa ccctgtgcga aaggtcgccg cttgcgacaa gcgcccagct
     2761 cccatgaaga ggcgccttca ggagagcctg tcccagccgg aacgcctcgg aaggagaaag
     2821 cccgatcggg tttgcgacaa caagtgaggg aagaggaatc ccgctctttt ggaggaaaaa
     2881 aaggggaacg aggctcccgt ggtcgagcgg gcactccttt cgcctgctcg ctactacagg
     2941 gaacgatttc tccagagtac gagcaagacc ttcgccctcc tctgaggccc ctctcaatga
     3001 aagacgagtc cggggagccg caaactgtgc aaaattgccc gaatactgct cggccagcga
     3061 gatcgtgagc cctcctgaaa aaggcgcgtg cggagagagc agaagaatgg tgtcaggcgg
     3121 attcgtgctc gcaagggagg aaaccgcgtt cattccttcg agcgtttgaa aggcatcgcg
     3181 ctctcttccc ctaccgacct ccgggacaat caccggagga tgaggcgtaa aacactccca
     3241 gtaccacatg acattcacct cctgaagagt cccatccggg ccgcgtactc taacagataa
     3301 acatcgcgtc cccgaacgag aaaaaacggt atttcatcga aacagcggtc tcatatgctt
     3361 tcattaccgg ttcatacccg gcaaatgcgg cgacaagcat cagtaatgag cttttgggaa
     3421 gatgaaaatt cgtcaccagt gcgtcaacaa ctttatattc aaaccccgga taaataaaaa
     3481 gttgtgtatt catctcaccc cagcgcatct ctccatcctc ggcagccatt gattcaaggg
     3541 tccgggcaac tgtcgtcccc gcagcgatta ccctgcctcc acgtcttttc gtctcctcca
     3601 caagtttttt cgtctccaga ggaagcacgc aatactcctc gtgaataaca tgcttacgaa
     3661 tatccgaaca ctgaaccgga cgaaatgtcc cgagcccgac atggagcgtc acccaggcga
     3721 ccgaaagacc gatctcccgc ctgagctctt ccagaagctc ctcggtgaaa tggagactcg
     3781 cagtcggcgc cgccgcggaa ccgtcccgtg cggcgaagac cgtctgatat gccgaacgag
     3841 gcgctgtaga gcgggtaata tacggaggaa gaggcgtctc cccgatttcc gtcaacaatg
     3901 aaagtacctt gacgccttga ggaaaagaga ctctccgcac cccttccgta ccttcaaaac
     3961 gttcgccaac agtaatcgct gttccatccg agagtatgat tcccgttccg gatcgaagcc
     4021 tccggccagg cttgacgagg gcctcccact cgctccactc cgggttgagc gctttcagaa
     4081 gga
//
LOCUS       DAOSWW010000116         4082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15152_length_4082_cov_3.791160, whole genome shotgun sequence.
ACCESSION   DAOSWW010000116 DAOSWW010000000
VERSION     DAOSWW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15152_length_4082_cov_3.791160"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PK442_03540"
     CDS             complement(<1..815)
                     /locus_tag="PK442_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO86751.1"
                     /translation="MRCGALRLRNFKNITTETILWGEGLNLLVGKNGAGKTNCLEALH
                     LLLGWGPLGDRKDIASWGSAEKKTYVTGDFFGEEEAFLAVGIGGSTVVKYNGKRCSFP
                     EVRSVAPSLAFLPSDMELLDGSPARRRSFLDRLCALLWPLYARRLVEFRRAVRHRIVL
                     LRQGGNLVALSKAMAPLASWLWGARASAVAALSEGLAALPDLLPLPVQLTHVRGGAGR
                     VKDPLEDWWESLDARREKERLCCSPLVGPHRDDLDVRCGDRSAASCLSRGQKRR"
     gene            complement(825..2003)
                     /gene="dnaN"
                     /locus_tag="PK442_03545"
     CDS             complement(825..2003)
                     /gene="dnaN"
                     /locus_tag="PK442_03545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOO86752.1"
                     /translation="MRLQISKGEFMKNWQIAERSTSSKSTINSLTGVLLRTADADNSV
                     LMEATDLKTSIKCISQGVLVEEEGEAVLPVKLVGELFKKAPTNVFTVSITDGKGIIIA
                     GRNKYKFTTYSPREFPKLPVSESAAFFCTATASELLRAVSEGTVACTTGEEFPKYLGT
                     AFFQLRDGEMRVVATDGRRLSLSKCSPVRTGENCEFLLPITGVRELQRLLGALDGESP
                     IEILVESTLVFFQMGSMEFSVRRVDTAFPNYENILNPQTTTTMDIDRHELIAALERVD
                     VIVRDFSHMVLVRLSPEGDLLLTGKAPETGAVQEVLDAKIEGESLTVAFNVSFLIEGL
                     KALYGDRVFISFNGPDGQMTMLRPGEKDFLYMAMPIKINESDLLHGEMDEENETEIPE
                     "
     gene            complement(2231..3559)
                     /gene="dnaA"
                     /locus_tag="PK442_03550"
     CDS             complement(2231..3559)
                     /gene="dnaA"
                     /locus_tag="PK442_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOO86753.1"
                     /translation="MVDNDLQHLWDHILDEAGSQLPAGATETWLRTCAPTEFTDGVLV
                     LDVPNVFVKEQISSRFLKELIHIFTETGGGRSIELRVGAETRKEERERAERAVSAGNL
                     ESRSGLNPGYLFNSFVVGKSNRLAHAASLAVAETPGEAYNPLFIWGGVGLGKTHLMHA
                     IGHYVLDRNSNSRVTYVSSEKFINEFIQSIKNNKTQEFKSKYRSVDILLIDDIQFLGN
                     KGSSQEEFFHTFNQLHTSKKQIIICSDRPPKEIQSIEDRLVSRFEWGLVTDIQSPDLE
                     TRIAILQKKAQLRRYNIPDDVINFLAINIPSNIRELEGALNRVVACAELAAEPINEEN
                     TREWLKDVIRKDLRGPVSIDSIQNTVADFFSMSVDELLSSKRTSDLAMARQIAMYLCR
                     KITESSLQQIGMAFKKKDHTTVLHAHRKIAQMIKEQPRIKQIVDTIEGKL"
BASE COUNT          823 a         1196 c         1148 g          915 t
ORIGIN      
        1 cgccgtttct gtcctctact cagacaggag gcggcggaac ggtcgccgca acggacgtcg
       61 agatcgtctc tgtgcggccc gacaagcgga gagcagcaga gacgctcctt ctccctccgt
      121 gcgtcgaggg actcccacca gtcttcgaga gggtctttca ccctccccgc gcctcccctg
      181 acgtgcgtca gctgcactgg aagcggcaga agatccggaa gcgccgcaag tccctccgaa
      241 agtgcggcga cagcggacgc tctcgccccc cagagccacg aggccagcgg ggccatcgcc
      301 ttggagagcg cgacgaggtt tcctccctgc cggagcagca cgatcctgtg ccgcaccgcc
      361 cgccggaact cgacgagacg ccgcgcatag agcggccaga gcagcgcgca cagacggtcg
      421 agaaacgaac gcctccgggc gggagatccg tcgagcagct ccatgtcgga aggaagaaac
      481 gcgagcgacg gagcgacgga acggacttcg gggaaggagc agcgtttccc attgtacttg
      541 acgacggtgc ttcctccgat gccgacggcg agaaacgcct cctcctcgcc gaagaagtcg
      601 ccggtgacgt acgtcttttt ctcggcgctg ccccaggaag caatatcttt cctgtcgccc
      661 agcggtcccc agccgagcag cagatgaagc gcctcaagac agttcgtctt cccggcgccg
      721 ttcttcccga cgagcagatt caatccttcg ccccacagaa tcgtctccgt cgttatattc
      781 ttgaagtttc gaagacgaag cgccccgcag cgcattcagc tcccctattc gggaatctcc
      841 gtctcgtttt cctcgtccat ttcgccgtgg agtaaatcgc tctcgttaat cttgatcggc
      901 atcgccatgt agaggaagtc cttctccccc ggtctgagca tggtcatctg tccgtccggg
      961 ccgttgaagc tgatgaagac gcggtcgcca tacagggctt tcagcccttc gatcaggaag
     1021 gagacgttga aggccacggt cagcgattct ccctcgatct tcgcgtcgag aacctcctga
     1081 acggctccgg tctcgggcgc cttccccgta aggaggagat cgccttccgg cgaaagacgg
     1141 acgagcacca tgtgactgaa atcgcgcacg ataacgtcca cgcgttcgag cgcggcgatc
     1201 agttcgtgcc tgtcgatatc catcgtcgtt gtcgtctgcg gattcagtat gttttcataa
     1261 ttcgggaatg cggtatccac tctgcggacg gagaactcca tagagcccat ctgaaagaac
     1321 acgagcgtgc tttcaacaag gatctcgatg ggagactcgc cgtcaagcgc gccaagaagc
     1381 cgttgcagct cgcggactcc cgtgatggga aggagaaatt cacagttttc tccggtgcgt
     1441 acgggggaac actttgacag ggagagacgt ctgccgtccg tggcgacgac gcgcatttct
     1501 ccgtctcgaa gctggaaaaa agccgttcca aggtacttcg gaaactcctc gcccgtagtg
     1561 caggcaacgg ttccctcgga gaccgcgcga agcagctccg acgccgtcgc cgtacagaaa
     1621 aaggccgcgc tttcggagac cggaagcttg ggaaactcac ggggagagta cgtggtgaac
     1681 ttgtacttgt tccgccccgc gatgatgatc cccttgccgt cggtgatgga gacggtgaag
     1741 acgttcgtcg gcgctttctt gaagagctcg ccgacgagtt tgaccggaag aacggcttct
     1801 ccctcctctt cgacgagaac gccctgcgag atgcacttga tcgaggtctt cagatccgta
     1861 gcctccataa gtacgctgtt gtccgcgtcg gcagtccgca gtaaaactcc tgtgagggaa
     1921 ttgatcgtac ttttagagct tgtgctcctt tcggcgatct gccagttttt catgaattct
     1981 cccttgctga tttgcagtct catgcctttc cctcccgggt ctcatctttt attgttcttt
     2041 atcttttagt agcagtaatg gtaataaggg gtgtgcgaat tgtggataac cgtccccgga
     2101 cctttggaag acgaacgact tcactgtgga cagacctggg cgattcattc agaaattatc
     2161 cacattgtcc acaggacatt ccgtacattt cttcattctg cgagagttat ccacatatcg
     2221 accccgagtt tcacagcttg ccctctattg tatccacaat ctgtttgatc cgcggctgct
     2281 ctttgatcat ctgcgctatc tttctgtgcg cgtggagcac cgtcgtgtgg tccttcttct
     2341 tgaacgccat gcctatctgc tgcaggctcg actccgtgat cttccggcag aggtacatcg
     2401 cgatctggcg ggccatcgcg agatcggacg tcctcttcga gctgagcagt tcgtccaccg
     2461 acatgctgaa gaaatcggcg acggtattct gaatggagtc gatgctcaca gggccgcgca
     2521 ggtcttttct gatgacgtcc ttcagccact cccgcgtgtt ctcctcgttg atgggctcgg
     2581 cggcgagttc tgcgcaggcc acgacgcggt tgagcgcgcc ctcgagctcg cggatgttgc
     2641 tcgggatgtt gatcgcgagg aagttgatca cgtcgtccgg gatgttgtag cggcggagct
     2701 gcgccttctt ctggaggatc gcgatgcgcg tctccaggtc gggcgactgg atgtcggtca
     2761 ccagccccca ttcgaaacgg ctcacaaggc ggtcctcgat gctctggatc tccttcggcg
     2821 gccggtcgga gcagatgatg atctgctttt tcgatgtgtg gagctggttg aacgtgtgga
     2881 aaaactcctc ctggctgctt cccttgtttc cgaggaactg gatgtcgtcg ataagcagga
     2941 tgtccacgct ccggtacttc gacttgaact cctgcgtctt gttgttcttg atcgactgga
     3001 tgaactcgtt gatgaacttc tccgagctga cgtacgtcac gcgcgagttc gagttccggt
     3061 cgagcacgta gtgcccgatc gcgtgcatga gatgcgtctt ccccaaaccg acgccccccc
     3121 agatgaagag cgggttgtag gcctctcccg gcgtttcggc cacggcgagg cttgcggcgt
     3181 gcgccagccg gttcgacttc cccacgacga acgagttgaa gaggtacccc gggttcaaac
     3241 cgctgcggga ctccaaattc ccggcggaga cagcgcgttc ggcccgttcc cgctcttcct
     3301 tccgggtttc ggcgcccacc ctgagttcga tcgaccgtcc cccgcccgtc tccgtaaaga
     3361 tgtggataag ctccttcaga aaccgggagg atatctgctc ctttacgaag acattgggga
     3421 cgtcgaggac gagcacgccg tccgtaaact cggtgggagc gcaggttctg agccacgttt
     3481 ccgtcgcccc cgcggggagt tgcgaaccgg cctcgtcgag aatatgatcc cacagatgct
     3541 gtaaatcgtt gtccacaata cttcacctca tgctgagaat ggcaccatat tatcacagag
     3601 actgtggata agctgcacat tcgcgcctga aacgggaaaa agccgccctt tcaatccatt
     3661 gtcgccttgg aaagagatac gttgtccaca aaagttatcc acatatccac aaaatgagtt
     3721 tcgttttgtg gatagggaga atggcggatg tggacggatg taaagaagtt gttacaaaat
     3781 tcgcggtctg tccacaaact cctctgcgct tgcaggaagc aaaatgagct ctttatccac
     3841 aaactttcca ccgttgtgga taaagggtgt ggatgcctgt gaatctacga attgctgttt
     3901 ctccaggtga accggggcgg gaaaaagaga cgccgccgtc cgtccggaaa ttcagttgcc
     3961 cgacagtttc aaagagcgga cggaaagggt agaatacaat acggagtctt tttcttcaca
     4021 ccatcgccgg acgacaccct gttccgtcaa aaaaggagaa taccaaatgg atcactcgct
     4081 gc
//
LOCUS       DAOSWW010000117         4078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15173_length_4078_cov_3.802386, whole genome shotgun sequence.
ACCESSION   DAOSWW010000117 DAOSWW010000000
VERSION     DAOSWW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15173_length_4078_cov_3.802386"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1362)
                     /locus_tag="PK442_03555"
     CDS             complement(211..1362)
                     /locus_tag="PK442_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO86754.1"
                     /translation="MKKAPWILAVLLVATLVTGSCAEEVIKVGYLAGITGDFAAYGQP
                     EVNAVKLAADEINAKGGVLGKKIELVIYDYKSRPEDAVNAARRLIDHDKVVAILGSAA
                     SGANIATAPLVTRAGIPQIGTLSTNPLVTVDESGNVRPYMFRICFIDPYQGRLIGYFA
                     AKELGKKKAAILYDVGSDYSHGLREHFISSYEEFGGTVVAEHAFRGGQDVDFRAQLTQ
                     IKQTDAEILVLPNIGKDLALPIKQARELGMEDIIFIGGDGYGEFMVEIAGEALEGSYW
                     MYHLARQDPAMKPFFDAYKEKYNGECQEFAIAVLADDSLYWLVDAIERAGSADSKAIR
                     DALEATKDLQLHHATLTMDPKTHNPVDKDAVVLTVQGGKSIFYKKIKPE"
     gene            1577..2806
                     /locus_tag="PK442_03560"
     CDS             1577..2806
                     /locus_tag="PK442_03560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOO86755.1"
                     /translation="MNAHASPVLLPGRGSERRNEIRRWCFLVLSVLLLFLSCTGRAVA
                     AAGLLPAEHAEESGSPEKISRFSFLAVGDALIHKGVYEAARKGKKQFDFRPMFRLVKE
                     RVQAHDVAFYNQETILGGVDLRLSTYPCFNSPQEVGDGMIDAGFNLVSLANNHTLDRG
                     EKAILASHEYWAGQDGVLTAGSYASLEDRETPRLIEVNGITCAFLAYTTLTNGLKAPK
                     DKEFYVAIYDKDSVEADVKRAREAKADVVIVSMHWGSEYVFTPGAQQKEIAGHLASLG
                     VNLVVGHHPHVVQSVQMVKDTLVIYSLGNFISSQKELFKLIGLMVSLDVVKKERGKEK
                     TISFENVTGTLLYNPRRSPLGRYAVVPFDLMKPDILKDFDKVHKKYAAYVKLDETLSI
                     RPPAKMPPKPPAKRRKK"
     gene            2876..>4078
                     /locus_tag="PK442_03565"
     CDS             2876..>4078
                     /locus_tag="PK442_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO86756.1"
                     /translation="MSIRLEEVIGKRLAGDQSQRVIWWKGTWWDAEKVEQKTEECASI
                     LREAGFQPGSRLAAFLPNSPLVLLLSMAAWRLGGTFVPLNPRGGVEWILRILDHLDPF
                     AVVVGEEMTPLEEAIRGAGIPVVAAPSDGVPPAFTGKRDRIGQDPDIAVIFATSGTTG
                     APKAVPLTHENLYDNTKGVHETIEGFDKGRVLMNVLPNFHSFGYTVCSVLPLLWGLPE
                     ALLPGFMPLKNLFEGLRDSGTGILIAVPTMLPFILGAASKGEKLPSELRYILTGGGKL
                     DPLLEKRFRDQLGVITYEGYGLTECSPVVSCNPSDALRKTGTVGPALPSYTVEVRGED
                     GHPVPPGQDGVLWLKGPSVAGGYFRDPELSAERFHDGWIDTGDMVRVDEDGYISILDR
                     VSDMIIVGG"
BASE COUNT          822 a         1163 c         1222 g          871 t
ORIGIN      
        1 ctacaaaatt cacgaggaag acgagtgctg gtcggtggag gatatcatga agaacgctca
       61 tatcatggcg aaagttctcg tcgaactcgc cgagaggaag taacctgcgc tcgcaggggg
      121 cgtagtacac agcacgcgta caaagagggg agagccggta ttcccgggct ctcccctctc
      181 cttgttcatg ctctgctgcc gaatctgatg ttattccggc ttgatttttt tgtagaatat
      241 gctctttccg ccctggacgg tgagcacgac ggcgtccttg tccacggggt tgtgggtctt
      301 cgggtccatc gtcaacgtcg catggtgaag ctgcagatcc ttcgttgctt caagggcgtc
      361 gcggatggcc tttgaatccg cgcttccggc gcgctcgatc gcgtcgacga gccaatagag
      421 gctgtcgtcg gcgagcacgg cgatggcgaa ctcctggcac tcgccgttgt acttctcttt
      481 ataggcgtcg aagaagggtt tcatcgccgg gtcctgacgg gcgagatggt acatccagta
      541 cgaaccctca agagcctccc cggcgatctc gaccatgaac tcgccgtaac cgtcgccgcc
      601 gatgaagatg atgtcttcca ttccaagctc cctcgcctgc ttgatcggaa gcgccaggtc
      661 cttcccgatg ttcggaagaa ccagtatttc ggcgtccgtc tgcttgatct gcgtcaactg
      721 ggcccggaag tcgacgtcct ggccgccgcg gaacgcgtgc tccgcgacaa ccgtgccgcc
      781 gaactcttcg tagctgctga tgaagtgctc gcgcaggccg tgggagtagt cgcttccaac
      841 gtcgtagagg atcgcggcct ttttcttgcc gagctccttg gctgcaaagt atccgatcag
      901 acgcccctgg tagggatcga tgaagcagat acggaacatg tacggacgga catttccgct
      961 ctcgtcaacg gtgacaagcg ggttagtgga gagcgtgccg atctgcggaa ttccggcgcg
     1021 cgtgacgagc ggcgcggtcg cgatattggc gccgctggcc gcggaaccga gaatcgcaac
     1081 aaccttgtcg tggtcgatca gtcttctggc ggcgttgacc gcatcttcgg gacgggattt
     1141 gtagtcgtag atgaccagct cgatcttctt gccgagaacg ccgccctttg cgttgatctc
     1201 gtccgccgcg agcttgaccg cgttcacttc cggctgaccg tacgctgcaa agtctcctgt
     1261 gattcctgca agatacccta ccttgataac ttcttcagca cacgaacctg ttacgagggt
     1321 tgctactaaa agtaccgcga gaatccatgg cgcctttttc atgaagcact cacctccggg
     1381 aattattgcg gttatgcgaa aatagagctc cgcacgaccg atccgatata gtatccgata
     1441 ttcgatatgc taatctctga gactctcttt gtcaatggag aagttcgata agttggattc
     1501 gcaacggaag agaagcctat ttctccgttc atgagggtat aatagacata gagcttttgc
     1561 cggaggtggt cctttgatga atgcacacgc ttcccccgtc ctgttgccgg ggagagggag
     1621 cgaacgccgg aatgaaatcc ggcgatggtg ttttctcgtt ctctcagttc tccttctctt
     1681 tctttcgtgc acgggacggg cggttgccgc cgccggtctt cttcctgcag agcacgcgga
     1741 ggagagcggg agccctgaga aaatctcacg cttttccttc ctcgccgtcg gcgacgcgtt
     1801 gattcataaa ggcgtgtacg aagcggcgcg aaaggggaaa aaacagttcg atttccgccc
     1861 gatgttccgg cttgtaaaag aacgggttca ggcgcacgac gttgccttct acaaccagga
     1921 gactatcctc ggcggcgttg acttgcgcct gtccacgtac ccgtgtttca actctccgca
     1981 ggaagtgggc gacggtatga tcgacgccgg cttcaacctc gtctcgctgg cgaataacca
     2041 cacgcttgac cggggggaga aggcgatcct cgcctcgcac gaatactggg cggggcagga
     2101 cggagtcctg acggccggaa gctatgcgtc gctggaggac cgggaaacgc cgaggctcat
     2161 cgaggtaaac ggcatcacct gcgccttcct cgcgtacacc acgctcacga acggtcttaa
     2221 ggcgcccaag gataaagagt tctacgtagc catctacgac aaggattcag ttgaggcgga
     2281 cgtgaagcgg gccagggagg cgaaagcgga tgtcgtcatc gtctccatgc actggggaag
     2341 cgaatacgtc tttacgcccg gagcgcagca gaaggagatc gccgggcatc tggcctcgct
     2401 cggagtgaat ctcgtcgtcg ggcatcaccc tcacgtggtg cagtccgtcc agatggtgaa
     2461 ggataccctt gtgatctact cgctggggaa tttcatctcc tcccagaaag agctcttcaa
     2521 actgatcggg ctgatggtgt cgctcgacgt ggtgaagaaa gagcgcggga aagagaagac
     2581 catctccttc gagaacgtga cggggacgct gctttacaac ccgaggcgtt cgccgctcgg
     2641 ccggtacgcc gtagtgccgt tcgacctgat gaaacccgat atactgaaag atttcgacaa
     2701 ggttcataaa aagtacgcgg catacgtgaa gctggacgag acgctttcga tccgtcctcc
     2761 tgcaaaaatg ccgccgaagc ctccggcgaa acggagaaaa aagtgatatg cgtctgttta
     2821 ttctgaaggg agccctcgac agagtgcgcc ttcgccgaga cagggagggt tttgtgtgtc
     2881 cattcgactt gaagaagtaa tcgggaaacg tcttgccggg gatcagtcgc agcgggtgat
     2941 ctggtggaag ggaacatggt gggatgcgga gaaggttgag caaaagaccg aggagtgcgc
     3001 gtccatactc cgtgaagcag ggttccaacc gggaagccgg cttgcggcgt ttcttcccaa
     3061 cagcccgctg gtcctccttc tctccatggc tgcctggcgg cttggaggga cttttgtgcc
     3121 cctgaatccg cgcggcggcg tcgagtggat tcttcgtatt ctcgatcacc tggatccgtt
     3181 cgcggtcgtc gtcggagagg aaatgacgcc tcttgaagaa gccatccggg gcgcagggat
     3241 acccgtcgtc gcggctccct cggacggcgt tcctcctgcg tttaccggca aacgcgacag
     3301 aatcggccaa gatcccgata ttgcggtgat ttttgctacc tcggggacga ccggcgcgcc
     3361 gaaagcggtg ccgctcaccc atgaaaatct gtacgacaac acgaagggcg tccacgaaac
     3421 cattgagggt tttgacaagg gacgcgtcct gatgaacgtc ctcccgaact ttcactcctt
     3481 cggctacacg gtgtgcagcg tgctcccgct gctctgggga cttccggaag ccctgctccc
     3541 cggcttcatg ccgttgaaaa acctcttcga aggactgcgc gactccggga ccggaatcct
     3601 gatcgccgtt cccacgatgc ttccgttcat cctgggcgcc gcctcaaagg gggagaagct
     3661 cccgtccgag ctgcggtaca tcctcaccgg cggcggaaag ctcgatccgc tgctcgaaaa
     3721 acgcttccgc gaccagctcg gcgtcatcac ctatgaaggg tacgggctta ctgaatgctc
     3781 ccccgtggtc tcgtgcaatc cgagcgacgc gctccggaag acgggaacgg tcggccccgc
     3841 gctgccgagc tatactgtgg aagttcgagg ggaggacggc catcctgtcc ctcccgggca
     3901 ggacggcgtg ctctggctta aaggaccgtc ggtggccggg ggctatttcc gcgatcctga
     3961 actcagcgcc gaacgcttcc acgacgggtg gatcgacacc ggagacatgg tcagggtgga
     4021 cgaagacggg tacatctcca tcctcgacag ggtctccgac atgattatcg tcggcggt
//
LOCUS       DAOSWW010000118         4077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15183_length_4077_cov_2.734709, whole genome shotgun sequence.
ACCESSION   DAOSWW010000118 DAOSWW010000000
VERSION     DAOSWW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15183_length_4077_cov_2.734709"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PK442_03570"
     CDS             complement(<1..990)
                     /locus_tag="PK442_03570"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO86757.1"
                     /translation="MRVLKDVDVDELHLFRGRVVEDVVSSTGSLLLPKGSEIQAVLES
                     VPGITKTLKRWGVYKVMLDVSFNISEEDFNKIIENIEPKIRVLEAALAQNTITQVDEV
                     YRRISENGSLGEGAYMLARQAALLTEEVTRAPQILLCLGRVRDSDEYTFVHSLNVGLL
                     CGYLAGRLKPGDREFAASMTYGGLLHDLGKAKVPQQVLNKPGKLTEKEYEMMKAHSIY
                     GDEIARSSGISDVRVLSVIRNHHERWGGHGYPDGLQKNSIPLFARIAAVADVFDALTA
                     KRVYKNPLSSREAVSMILESSENDFDKVVVRELLLSVGLYPPGTLVELSDFSVG"
     gene            complement(1027..1869)
                     /locus_tag="PK442_03575"
     CDS             complement(1027..1869)
                     /locus_tag="PK442_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO86758.1"
                     /translation="MEHKFSQVIHQLGGAYFANTLMNSFMAQLDEVLVSRVRAVEEEI
                     DATSERFVQLSALLGDLLKEFEKSGKETRESVGRITQMNEDLGEELKRSGTDLDGMSE
                     NVSRTVDATFGTLESFLEIEKMSKEIQRIAKQTNLLALNASIEAARAGEHGRGFAIVA
                     KNVQELAAETKAASESIGSKVGEISGAVQNAMEDIRRVSEMFDVIRRSLSTFTEFLST
                     NKSFMERMEHTMADAGDKVRRSSEEMDNSVEVMGNASKKFGSMAATISAIVLAQKNLK
                     QIRL"
     gene            complement(1887..2660)
                     /locus_tag="PK442_03580"
     CDS             complement(1887..2660)
                     /locus_tag="PK442_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO86759.1"
                     /translation="MQHVSSLTLYDQGDHKFIMLGWEEQEEEVAVQTNQYVISSGGEV
                     LLLDPGGAHVFPRVLANVAEMFELKDIRHIFYSHQDPDVSSGITLWLSMAEKATVYIS
                     GLWVRFLPHFGIYDVKRIKAIPDKGDAISLSNGKKMTVIPSHFLHSTGCFSLYDPTSK
                     VLFSGDIGAAIFPRGTRYPVAEDFDAHLRYMEGFHKRYMASNAFCRRWVKTVSALDVE
                     HIAPQHGALIKGRENVKKFLAWFENLACGVDVVDDIYGR"
     gene            complement(2737..>4077)
                     /locus_tag="PK442_03585"
     CDS             complement(2737..>4077)
                     /locus_tag="PK442_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO86760.1"
                     /translation="DAQIELRSNYDYLTGLPNKEMFQSRLSGELAHRERNSERLALLV
                     FDINRFKNINDTLGHFVGDSVLRKVGSLLSECIGSGGLVSRIGGDDFHILLPDVHTPD
                     EIVGQVKRVFECLSDPVDVEGHRLFLSASVGISVFPEDGRDVDDLLKRADIAMGKAKE
                     SGISSYRFFTEELGRRASLKLTLENALREGLERNEFHLHYQPKYCIRRGAFSGAEALL
                     RWNSPEGPVPPSDFIPLAEETGLILSLGEEVFRKVCRQIAEWRSRGYSPGEIAVNLSA
                     RQFHQKGLLSRMKGILGEYDVPPSLLGIEITESGIMENLMDSIAVLSGMKDLGMTVYV
                     DDFGTGYSSLNYLKRLPIDVLKIDKSFIDGVLEDPNDAAITRAVVGIARSLGLRVVAE
                     GVETPEQLDFLRRNGCDEIQGYLLSRPLPPDAFEKFGESEIPLCFNGLSGYNHC"
BASE COUNT          833 a         1282 c         1102 g          860 t
ORIGIN      
        1 tcccacgctg aaatccgaaa gctccaccag cgtacccggt gggtacaacc ccacggagag
       61 caacagctcc ctaacgacga ctttgtcgaa atcgttttcg gaggattcca gaatcatcga
      121 caccgcttcg cggctcgaca gcggattttt gtacactctc tttgctgtga gcgcgtcgaa
      181 gacatcggca accgctgcga tgcgggcaaa cagcgggata ctgttctttt gcagaccgtc
      241 ggggtaccca tgccctcccc aacgctcatg gtgattccgg atgacagaaa gcacgcggac
      301 gtcgctgata ccagatgacc tggcgatttc atctccgtag atggagtgcg ccttcatcat
      361 ctcgtactcc ttctccgtca gttttcccgg cttgttgagc acctgctgcg gaaccttggc
      421 tttaccgagg tcgtgaagga gtccgccgta cgtcatcgag gcggcgaact cgcggtcgcc
      481 gggtttcaga cgtccggcaa gataaccgca gagcagcccc acgttgagtg agtggacgaa
      541 tgtgtactcg tcgctgtcgc gcacccgccc caaacagagc aaaatctgcg gagcgcgcgt
      601 aacctcttcg gtcagcaacg cggcctgcct cgccagcatg taggcgcctt ccccaaggga
      661 accgttctcg gagatccgcc gatagacttc gtcgacctgc gtaattgtat tctgtgcgag
      721 cgcggcctcc aatacacgga tcttcggctc gatattctcg attatcttgt tgaagtcctc
      781 ctccgaaatg ttgaaggaga cgtcgagcat aaccttatag acgccccagc gcttgagcgt
      841 ctttgtaatc ccgggaacgg actccagaac ggcctgaatc tcgctcccct tcggtaaaag
      901 aagcgaaccg gtcgacgaca ccacatcttc gacgacccgt ccgcggaaca ggtgtaattc
      961 gtcgacatcc acatccttca gcacccgcac ggcaaccccg ccttctctcc cgccgaaacc
     1021 tccggcttaa agtcgtatct gcttcaggtt cttctgggca aggacgatcg cggatatggt
     1081 cgccgccatc gaaccgaact tcttcgacgc gttccccatc acctcgacgg agttgtccat
     1141 ctcctcggaa ctgcggcgga ccttgtcacc cgcgtcggcc attgtatgct ccatacgctc
     1201 catgaacgat ttgttggtcg acaggaactc ggtgaacgtc gaaagcgaac gccggatgac
     1261 gtcgaacatc tcgctcaccc gccggatatc ctccatggcg ttctgcaccg cgccggagat
     1321 ctcgcccacc ttggagccga tggattcgga agccgccttg gtctccgcgg cgagctcctg
     1381 gacgtttttc gccacgatgg cgaacccccg cccgtgctcg ccggcccgtg cggcctctat
     1441 ggaagcgttg agcgccaaca gattcgtctg cttggcgatg cgctgtatct ccttggacat
     1501 cttctctatt tccaggaagg actccagagt gccgaacgtg gcgtctaccg tccgggagac
     1561 gttctcgctc atgccgtcga ggtcggtgcc ggagcgtttc aactcctccc ccaggtcctc
     1621 gttcatctgg gtgatgcgtc cgacgctctc tctggtctcc ttcccgctct tttcgaactc
     1681 tttcaacaga tccccgagca gggcgctcaa ctggacgaag cgctccgagg tcgcgtcgat
     1741 ctcctcctcg actgccctga cgcggctcac cagcacttcg tcgagctgcg ccatgaagga
     1801 gttcataagc gtgttggcga agtacgcccc tccaagctga tggattacct gtgaaaattt
     1861 atgctccatc ccccgaccct ccccggctag cgaccgtaga tgtcgtcgac gacatctacg
     1921 ccgcaggcga ggttttcaaa ccacgcgagg aacttcttca cgttctccct ccccttgatc
     1981 agcgcgccgt gctgcggggc gatgtgctcc acgtcgagcg ccgataccgt cttcacccat
     2041 ctgcggcaga aggcgttcga cgccatatag cgcttgtgga acccctccat gtacctcagg
     2101 tgcgcgtcaa aatcctcggc tactgggtag cgggttccgc gcgggaagat cgccgcgccg
     2161 atatcgcccg agaagagaac cttcgacgtc gggtcgtaca gggaaaaaca tcctgtagag
     2221 tggaggaaat gagaagggat gaccgtcatc ttcttgccgt tggagaggga gatcgcatcc
     2281 cccttgtcgg gaatggcctt gattcgtttc acgtcgtaga tgccgaagtg cggaaggaag
     2341 cgcacccaca gcccggagat atacaccgtc gccttctcgg ccatcgacag ccagagcgtg
     2401 atgcccgagg agacgtccgg atcctgatgg gagtagaaga tatgccggat atccttcagt
     2461 tcgaacatct cggccacatt cgccaacacc ctcgggaaga cgtgcgctcc gcccggatcg
     2521 agcagcaaca cctcgccgcc ggaagagatg acgtactggt tcgtctggac ggcgacctct
     2581 tcttcctgct cctcccagcc gagcatgatg aacttgtgat ccccctggtc gtagagggta
     2641 agcgaactca cgtgctgcac tccaaccaac tccttccgta tgcagaggag acggacgttt
     2701 ctccggggta tattctattc tacggagcac cccgtctcaa cagtgattat aacctgagag
     2761 tccgttgaaa caaagaggaa tttcactctc cccgaatttt tcaaaggcat ccggcggaag
     2821 aggccggctc agcaagtacc cctggatctc gtcgcatccg ttgcggcgca aaaaatcaag
     2881 ctgctccgga gtctccaccc cttccgctac aacgcgcagc cccagcgaac gggctatgcc
     2941 gacgacggcc ctggtgatgg cggcgtcgtt gggatcctcc agaacgccgt cgataaaact
     3001 cttgtctatc ttcagtacgt cgatgggaag gcgtttaaga tagttcagcg acgaataccc
     3061 ggtgccgaag tcgtccacgt acacggtcat ccccaagtct ttcatcccgg aaagcacagc
     3121 gatggaatcc atgaggttct ccatgatgcc ggactcggtg atctcgatcc cgagcagaga
     3181 gggcggcaca tcgtactcgc caagaatacc tttcatcctc gagagaagcc ctttctgatg
     3241 aaactggcgg gccgaaagat tcacggcgat ctcgcccgga gagtagccgc gactcctcca
     3301 ttcggctatc tggcggcaca ccttcctgaa gacctcttct cccagagaga ggatgagccc
     3361 cgtctcctcc gcaagcggaa taaagtcgga gggcggcacg gggccttccg gactgttcca
     3421 gcgcaggagc gcttccgccc cggagaaagc cccccggcga atgcagtact tgggctgata
     3481 gtgaaggtga aactcattcc tttcgagccc ctcgcgcagc gcgttctcca gggtgagctt
     3541 cagcgacgcc cgtcttccaa gctcttccgt gaagaatcgg tagctgctga ttccgctctc
     3601 ctttgccttc cccatcgcga tatccgcccg tttgagcagg tcgtcaacat ccctgccgtc
     3661 ctcgggaaag acgctgatcc cgacgctcgc gcttaaaaag agacgatgcc cttcaacatc
     3721 cacggggtcg ctgagacatt cgaagacccg tttgacctgg ccgacaattt catccggggt
     3781 atggacatcc gggagcagaa tatgaaaatc gtccccgccg atacgggaga ctaatccgcc
     3841 tgaaccgatg cactcggaga gcagactgcc gacttttcgc agtaccgaat cgccgacgaa
     3901 atgaccgagc gtatcgttga tattcttgaa acggttgatg tcgaagacaa gcagcgcgag
     3961 ccgctcgctg tttctctccc ggtgggcgag ttcgccggag agacggctct ggaacatctc
     4021 cttgttcggc agaccggtga gatagtcgta gttggagcgg agctctatct gggcgtc
//
LOCUS       DAOSWW010000119         4057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15305_length_4057_cov_3.158921, whole genome shotgun sequence.
ACCESSION   DAOSWW010000119 DAOSWW010000000
VERSION     DAOSWW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15305_length_4057_cov_3.158921"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..978
                     /gene="cbiB"
                     /locus_tag="PK442_03590"
     CDS             52..978
                     /gene="cbiB"
                     /locus_tag="PK442_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOO86761.1"
                     /translation="MEVFVVLFAAAVWDLLLGEPSSKLHPVVWMGKYIGAFWNRRPSG
                     AEDRKMLIFGGVLVVSGALLFSLPLRFLKLLPGLTALLLTIPLLKSVFSISALINAGE
                     SILRALELGDVPEARRLLSWSLVSRNTEDLSQEEVVGAAIESLAENLTDSLASPLFFY
                     AIFGLPGAFFYRFCNTCDSMIGYRGGDTEWGGKIAAKLDDVLNWIPARVTSILLLGAG
                     VLAKEDVIKGAEALRDERGNTASPNAGWTMSVMAGMLRVTLEKRGQYALAGGTEPLCT
                     QALRRCVKVVRIAAALILAFVAALGGLHALFG"
     gene            962..2056
                     /locus_tag="PK442_03595"
     CDS             962..2056
                     /locus_tag="PK442_03595"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOO86762.1"
                     /translation="MPFSVRKELLLAAETVHGGLDYAKLRSEGVEPESILDFSVSTNP
                     LPPHPEVERAVARAAFDRYPDSSSGALRRAIAEANGLSPDEVLVTNGLAQAIVLLAFA
                     FCDRGSRALVASPAFGEYEAASRLAGAEVLFVPAREEEEFAFPAERLMQAVRAHRPAL
                     VWVCSPNNPTGAAPSREEAVSLLRVCEDEGALLLLDEAYVNFAPPGVSARGLFPSPNL
                     LLLRSMTKDYGLTGLRLGYILGATGLLRPLRALQPPWSVNACAQAAGEEALRQNAYYE
                     AQWREVRSLTERMASKLRALGYAPSPPSANFILFKVGDTAALKEFLWRERILVRDCAS
                     FGLPGYVRVGVRSEAENNRLIECISSFKER"
     gene            2062..2610
                     /gene="cobU"
                     /locus_tag="PK442_03600"
     CDS             2062..2610
                     /gene="cobU"
                     /locus_tag="PK442_03600"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOO86763.1"
                     /translation="MGKITFVLGGARSGKSAWAEARALEMERSGSSVVYLATCEYRAD
                     DAEMCDRISRHRSRRPSAWRTVEEPYGIEDVVARLDAGSVALVDCLSLWVSNLLLMTP
                     EQGDSGIADSVLGRVRAFLDAAKRSSGDVIVVSNETGCGVVPSSHLGRLYRDILGWAN
                     QEAAQAADEVWFLVAGLPQRLK"
     gene            2696..3778
                     /gene="cobT"
                     /locus_tag="PK442_03605"
     CDS             2696..3778
                     /gene="cobT"
                     /locus_tag="PK442_03605"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOO86764.1"
                     /translation="MTTLQQTIDKIRPLCAESMEAARRRQDVLTKPKGSLGRLEELSV
                     RLAGIQRTVHPVIRDKVIMTMAGDHGVVSEGVALYPKEVTVQQVLNFLQGGGGVNVLG
                     RHAGARVVLVDMGVDYDFEPMEGLQIRKVARGTASMLRRPAMTREQAVQALENGIAAV
                     YEEAVKGMDILGTGEMGIGNTTPSTAIACAVTGVAVPLATGRGAGLTDEGLKRKIEVI
                     EKSLALHSPDPKDGIDVLSKVGGFEIGGIAGAILGAASLGIPVVVDGFISTAGAVVAA
                     LLCPRAKEYMIYSHNSVEIGHEHMFSFLEARPLLDLGMRLGEGTGAALAISIVEGAAK
                     VLNEMATFESAGVSGPVDSEGVADEG"
     gene            3780..>4057
                     /locus_tag="PK442_03610"
     CDS             3780..>4057
                     /locus_tag="PK442_03610"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOO86765.1"
                     /translation="MRILEGFFRALSFLTILPCPKRESTVEEYGRASGWFPVIGAVLG
                     AILYASALLFHRLFSPLPASALVVAIWAFVTRGFHLDGLADTFDGIGGG"
BASE COUNT          661 a         1208 c         1423 g          765 t
ORIGIN      
        1 cctggcggcc gggggaccgc ggtaggatcc gttcgaaagg aaggtattcg catggaagta
       61 ttcgtcgttc tcttcgccgc cgcagtctgg gatctcctgc tcggcgagcc ctcgtcgaaa
      121 ctgcatccgg tggtctggat gggaaagtac atcggcgcgt tctggaaccg tcgtccgtcg
      181 ggagcggagg acaggaagat gctcatcttc ggcggcgtgc tcgtggtgtc gggggcgctg
      241 ctcttctccc ttccgctgcg ttttttgaag ctccttccgg ggttgacggc gctgctgctc
      301 accattccgc tgctgaagag cgtcttttcc atctccgcgc tgatcaacgc aggagagagc
      361 atcctgcggg cgcttgaact cggcgatgtg ccggaggccc ggcggctgct ttcgtggagc
      421 ctggtcagcc ggaacacgga ggatctgtcg caggaggagg tcgtcggcgc ggcgatagag
      481 tcgctggccg aaaacctcac cgacagcctg gcttcgccgc tcttcttcta tgcgatcttc
      541 ggccttccgg gggccttctt ttaccgattc tgcaacacct gtgactccat gatcggctac
      601 cggggagggg atacggagtg gggagggaag atcgccgcga agctcgacga cgtcctgaac
      661 tggattccgg ctcgcgtgac ctcgattctg ctccttgggg cgggcgtgct cgccaaggag
      721 gacgtcatca aaggcgccga ggcgctgcgc gatgaacggg ggaacaccgc cagccccaac
      781 gcggggtgga ccatgtcggt gatggcgggg atgctcaggg tgacgctgga gaaacgcggc
      841 cagtacgcgc ttgccggagg gaccgagccg ctgtgcacgc aggcgctgcg ccgctgcgta
      901 aaggtggttc gcatcgccgc cgcgttgatt ttggccttcg tcgcggcgct gggagggctc
      961 catgcccttt tcggttagaa aggaacttct gctcgccgcg gagacggtcc acggcgggct
     1021 cgactacgcg aaactgcgga gcgaaggggt ggagccggag agcattctgg acttcagcgt
     1081 ctcgacgaac ccgttgcccc cgcaccccga ggtggaacgg gccgtcgcgc gggcggcgtt
     1141 cgaccggtac cctgactcct cgtcgggggc gcttcggcgc gccatagccg aggcgaacgg
     1201 tctctcgccc gacgaagtgc tggtgacgaa cggattggcc caggcgatcg ttctccttgc
     1261 cttcgcgttc tgcgacaggg ggagccgcgc gctcgtcgcg tcgccggcct tcggggagta
     1321 cgaggccgcc tcccgtcttg ccggggccga ggttctgttc gtcccggcga gggaggaaga
     1381 ggagttcgcc tttcccgccg aacggctgat gcaggctgtg agggcgcatc ggcccgcgct
     1441 ggtctgggtc tgctcgccga acaatccgac gggcgcggcg ccgtcgcggg aagaggccgt
     1501 ctcgctgctg cgcgtctgcg aagatgaggg ggcgctcttg ctgctcgacg aagcctacgt
     1561 caatttcgct cctcccggtg tttccgcgcg cggtcttttc ccgtcgccga acctgctgct
     1621 gcttcgttcg atgacgaagg actacggtct gacggggctg cgactcggct atatcctggg
     1681 agcgaccggc ctgctccgcc cgttgagggc gctgcaaccg ccgtggagcg tgaacgcctg
     1741 cgcccaggct gcgggtgagg aagccctccg gcagaacgcg tactacgagg ctcagtggcg
     1801 cgaggtgcgt tcgctgactg aacggatggc ctcgaagctg cgcgctcttg ggtatgcgcc
     1861 gtcgcctccg tcggcgaact tcatcctctt taaggttggc gatacggcgg cgctcaagga
     1921 attcttatgg agagagcgca tcctcgtccg cgactgcgcc tccttcggcc tgcccggata
     1981 cgtacgggtc ggcgtgcgtt ccgaggccga gaacaaccgg ctgatcgaat gtatttcctc
     2041 gtttaaagag aggtgacgtc catgggaaaa ataaccttcg tcctcggcgg cgcgcggagc
     2101 gggaagagcg cgtgggccga ggcgcgggcg cttgagatgg aacggagcgg ttcttccgtg
     2161 gtttatctgg cgacctgcga ataccgcgcc gacgacgcgg agatgtgcga caggatctcg
     2221 cggcaccgtt cgcggcgtcc gtccgcgtgg aggaccgtgg aagagccgta cggaatagag
     2281 gacgtcgttg cacggctgga cgcggggagc gtcgcgctcg tggactgcct gagcctctgg
     2341 gtgagcaacc tccttctcat gacgccggag cagggcgact ccggaatcgc agattccgtt
     2401 ctgggacgag tccgggcgtt tctcgacgcg gcgaaacgct cctccggcga cgtgatcgtc
     2461 gtctccaacg agacggggtg cggcgtcgtt ccttcttctc accttggaag actctaccgc
     2521 gacattctgg gctgggccaa ccaggaggcg gctcaagccg ccgacgaggt ctggttcctc
     2581 gtcgcgggac tgccgcagcg gctgaaataa cgtagcacgc aacggaaacg tgcgtactca
     2641 cgcacgtcct tgcgcaatca tctcccgtgc acgcgggaaa ttcttaggag gcatcatgac
     2701 aactcttcaa cagacaatcg ataaaatccg cccgctgtgc gctgaatcaa tggaggcggc
     2761 gcgccgccgc caggacgtgc tcaccaagcc gaaggggagc ctcggcaggc tcgaagagct
     2821 ttcggtcagg ctcgccggta ttcagcggac tgtccacccg gtgatccgcg acaaggtcat
     2881 tatgactatg gcgggggatc acggcgtcgt ttccgagggc gtggcgctct atcccaaaga
     2941 ggtgactgtc cagcaggtgc tcaacttcct gcaaggcgga gggggcgtga acgtgctcgg
     3001 ccgtcatgcc ggagcacgcg tcgttcttgt cgacatgggg gtcgattacg acttcgagcc
     3061 tatggaaggc ttgcagatac gcaaggtggc gcgcggaacg gcgagcatgc tcaggaggcc
     3121 ggcgatgacg agggagcagg ctgttcaggc tcttgagaac ggaatcgccg ccgtgtacga
     3181 agaggcggtc aaagggatgg acatcctcgg aaccggggag atgggcatcg gcaacaccac
     3241 gccatcgacg gcgatcgcct gcgccgtgac gggcgtcgcc gttcccctcg ccacgggcag
     3301 gggagcgggg ctcaccgacg aagggctgaa acgcaagatc gaggtcatcg aaaagtcgct
     3361 cgccctgcac agtcccgacc cgaaggacgg tatcgacgtg ctctccaagg tcggagggtt
     3421 cgagatcggc ggtatcgccg gggcgattct cggcgcggcg tcgctgggta tccctgtggt
     3481 cgtggacggc ttcatctcca ctgcgggcgc ggtggtcgcg gcgcttctct gcccgagggc
     3541 gaaggagtac atgatctact cgcacaactc ggtggagata gggcacgagc acatgttcag
     3601 cttcctcgaa gcgaggccgc tgctcgacct cggcatgcgg ctgggcgagg gaaccggagc
     3661 ggcgctcgca atttcgatcg tcgaaggcgc cgcgaaggtg ctcaacgaga tggccacatt
     3721 cgagagcgcg ggcgtctcgg gaccggtcga ctccgaagga gttgccgacg agggctgata
     3781 tgcgaattct tgagggcttt ttccgtgcgc tgagcttcct gacgattctt ccgtgtccga
     3841 agcgcgagag cacggtggag gagtacgggc gggcgagcgg atggttcccg gtgatcgggg
     3901 cagtgctcgg ggcgattctc tacgcctctg cgctgctgtt tcaccgtctg ttttcgccgc
     3961 tgcccgcgtc ggctctcgtt gtggccatct gggcgttcgt aacgaggggg tttcacctcg
     4021 acgggctggc ggacactttc gacggcatcg gaggcgg
//
LOCUS       DAOSWW010000120         4052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15327_length_4052_cov_3.832624, whole genome shotgun sequence.
ACCESSION   DAOSWW010000120 DAOSWW010000000
VERSION     DAOSWW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15327_length_4052_cov_3.832624"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2125
                     /locus_tag="PK442_03615"
     CDS             <1..2125
                     /locus_tag="PK442_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOO86766.1"
                     /translation="QGCGMLFGTCLWDQPPLAVDKVRHAGEPVAVVLAETGNQAAAAL
                     KEIAVEYKKLPFVLDPVEAAAPGAPLIHEKNGEYRRVEYSVHPIPGSNVFHHYKLRKG
                     NEQEGFAKADVTVEDEFEFPLSSQAPLEPHGAICRFGTDGDIEIWASNQAPFVLRDVL
                     ADMFGIPATKVRVHIPYLGGGFGGKSDVSIEPMVAYAASFVPGYAVKLVLTRGEVISG
                     ALIGRGMKGRMRIGAKLDGTFTALKAEMYFADGAYGDTGWPVDTVAGHNCTGPYEFPN
                     AIVDVYGVYTNSPIVGAYRGYGHPEGQFMSSRLIDMLARKLDMTPAEIMRKNFLCEGR
                     KNALGQTIKKSNGDLFGCLDAVEKALAEEPLPPNDERYVYGRGIASMMKSPKMAANAA
                     SCCHVQVNADGSAFVNLAGIEMGQGVHTVFAQMAAEALELPFEKVRIYFDVDTQYSPW
                     EWQTVASMQTYRGGRAIQDACRALVEKAKKTAATAWNCAPGMVEYREGVCVHPNTGAT
                     LSLGTLARGFMAANGLTVGEPLAATGWYRVPEITEPDPETGMGNAAGSWTFGAQGCAL
                     RVDRQTGEVKILHFASAFDVGKAVNPAATRGQVTGGVVQGMGAALMEKILFTDEGVAK
                     NVTFPAYKIPRLHDAPEKQTVVLLETPNEEGPWSAKPMAEHPIVAVAPTILNALRDAT
                     GVEFTAIPVTPERILEALKTRKEDK"
     gene            2127..>2596
                     /locus_tag="PK442_03620"
     CDS             2127..>2596
                     /locus_tag="PK442_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO86767.1"
                     /translation="MRVLNFTVNGTPRTVHLEEGRVMLASVLRDSLGLTGTKVGCGTG
                     QCGSCTVVMDGKAVTSCTVPAEKAEGTSVLTIESLAEGGVLHPIQQAFVEAGAIQCGF
                     CTPGIVMRLYALFTQNPDADDETIMKELDKHLCRCTGYENIVKAAHLAQEKLRAA"
     assembly_gap    2597..2696
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2719..3927
                     /locus_tag="PK442_03625"
     CDS             2719..3927
                     /locus_tag="PK442_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO86768.1"
                     /translation="MAFSLLIKNARTRFSQGKTLSIGIEGEKIAAVGENLPETGAAQI
                     IDAGGKLVTESFVNGHLHLCKVYTLEMVGQDALSSYHGGSMGGAMTAIEQASRVKDHY
                     DEKWIIENVRKACRLAQKYGNTHIRAFADTDTKAKLEGIKALLKAREEFKDTVDLQVV
                     AFPQDGVVRDPGADGYIRSALELGADVVGGIPWIEYTDADMQAHIDSMFALAKEFGKD
                     VSMLIDDAGDSGLRSLEMLAVKTIKEGWQGRVTAQHCRAMAMYQEPYFRKVLALLQKA
                     KIGLVSDPHTGPLHARVRDLYDAGVAVALGQDDIADAYYPFGRNNMLEIAFLAVHLLW
                     MTTFSDMEILYDLITTNAAKAMGIKGHVLEPGGAADLVVLNAEDVYHAIWEHEAPLHV
                     IRKGKDVTLK"
BASE COUNT          794 a         1295 c         1298 g          565 t
ORIGIN      
        1 gcaggggtgc ggaatgctct tcggcacctg cctctgggac cagccgcccc tggccgtcga
       61 caaggtccgc cacgcgggcg agcccgtcgc ggtcgtcctt gccgagaccg ggaatcaggc
      121 ggccgcggcg ctcaaggaga tcgccgtcga gtacaagaag ctcccgttcg tcctcgaccc
      181 tgtcgaagcg gcggctcccg gcgcgccgct catccacgag aagaacggag agtaccggag
      241 agtggaatac tccgtccatc cgatccccgg aagcaacgtc ttccaccact ataagctccg
      301 caaggggaac gaacaggagg ggttcgccaa agccgacgtc acggtcgagg acgagttcga
      361 gttccccctg tccagccagg cgccgctcga gccgcacggc gcgatctgcc gcttcggcac
      421 ggacggcgat atcgagatat gggcctccaa ccaggccccg ttcgtcctcc gggacgtcct
      481 cgccgacatg ttcggcatcc ctgcgacaaa ggtgcgcgtg cacatcccct acctcggcgg
      541 cggtttcggc gggaagtccg acgtgagcat cgaaccgatg gtcgcctatg cggcgagctt
      601 cgtccccgga tacgccgtca agctggtact cacgcgcggc gaggtcatct ccggagcgct
      661 gatcggccgc gggatgaagg ggcgcatgcg gatcggcgcg aagctcgacg gcacgttcac
      721 cgcgctgaag gccgagatgt acttcgcgga cggcgcgtac ggcgacacgg ggtggcccgt
      781 ggacaccgtc gccggacaca actgcaccgg cccttacgaa tttcccaatg cgattgtcga
      841 cgtctacggc gtctacacca attcgccgat cgtcggcgcg tacagagggt acggacaccc
      901 cgaggggcag ttcatgtcca gccgcctgat cgacatgctt gcgcgaaagc tcgacatgac
      961 gccggcggag atcatgcgga agaacttcct ctgcgagggg cggaagaacg ccctcggaca
     1021 gacgatcaag aagagcaacg gagatctctt cggctgcctc gacgccgtcg agaaggctct
     1081 cgcggaggag ccgctcccgc ccaacgacga gcgctacgtc tacggcaggg gaatcgcctc
     1141 gatgatgaag tcgcccaaga tggcggcgaa cgcggcgtcg tgctgccacg tgcaggtcaa
     1201 cgccgacggc agcgccttcg tgaaccttgc gggaatcgag atggggcagg gcgtccatac
     1261 ggtcttcgcg cagatggcgg ccgaggcgct cgaactgccg ttcgagaagg tgcgcatcta
     1321 cttcgacgtg gacacgcagt actccccgtg ggagtggcag acggtggcct ccatgcagac
     1381 ctatcgggga ggacgcgcca ttcaggacgc ctgccgcgcg ctcgtcgaaa aggcgaagaa
     1441 aacggcagca acggcatgga actgcgcccc cggcatggtc gaatacagag aaggcgtatg
     1501 cgttcacccg aacaccgggg cgacgctctc cctcgggacg ctcgcgcgcg gcttcatggc
     1561 cgcgaacggc ctcacggtcg gcgaaccgct tgccgcgacc ggatggtacc gcgttccgga
     1621 gatcacggag cccgaccccg agaccggcat ggggaacgcc gccggaagct ggaccttcgg
     1681 cgctcaaggg tgcgcgctcc gcgtcgacag gcagaccggc gaggtgaaga tactccactt
     1741 cgcctcggcg ttcgacgtcg gcaaggcggt caaccccgcc gcaacgcgcg gacaggtcac
     1801 gggcggcgtc gttcagggaa tgggcgcggc gctgatggag aagatcctct tcaccgacga
     1861 aggtgtggcg aagaacgtca ccttccctgc gtacaagatt ccgcgtctcc acgacgcgcc
     1921 ggagaagcag accgtcgtcc tgctggagac gcccaacgaa gaggggccgt ggagcgcgaa
     1981 gcccatggcg gaacacccca tcgtcgcggt ggcgcccacc atcctcaacg cgctgcgcga
     2041 cgcgacgggc gtcgagttca cagcgattcc ggtcacgccg gagagaatac tggaagccct
     2101 gaaaacccga aaggaggaca aatagcatgc gcgtcctcaa cttcacagtc aacggaacac
     2161 cccggacggt ccatcttgaa gaggggcgcg tgatgctcgc ctccgtgctg cgcgactcgc
     2221 tcgggctgac gggaaccaag gtggggtgcg gcacggggca gtgcggctcg tgcaccgtgg
     2281 tgatggacgg gaaggccgtg accagctgca ccgtccccgc ggaaaaggcg gaagggacga
     2341 gcgtcctcac catcgagtcc ctcgccgagg gcggcgtcct ccacccgatc cagcaggcct
     2401 tcgtcgaggc cggggccatc cagtgcggct tctgcacgcc ggggatcgtc atgcgcctct
     2461 acgcgctctt cacccagaat ccggacgccg acgacgaaac gatcatgaaa gagctcgaca
     2521 agcacctctg ccgctgcacc ggctacgaga acatcgtcaa ggcggcgcac ctcgcgcagg
     2581 agaagctccg cgcggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa
     2701 tgaaaggaga atgaactcat ggcattcagt cttctgatca agaacgcacg gacccgcttt
     2761 tcacagggaa agaccctctc catcggcatc gagggggaga agatcgccgc cgtcggcgag
     2821 aacctcccgg agacgggcgc tgcgcagatc atcgacgcgg gcgggaagct ggtcacggag
     2881 tccttcgtga acggccatct ccacctgtgc aaggtgtaca cgctggagat ggtggggcag
     2941 gacgcgctct cctcctacca cggcgggagc atgggcgggg ccatgacggc catcgagcag
     3001 gcctcgcgcg tgaaggacca ctacgacgag aagtggatca tcgagaacgt ccgcaaggcg
     3061 tgccgcctgg cccaaaaata cggcaacacc catatccgcg ccttcgccga caccgacacc
     3121 aaggcgaagc tggaggggat caaggcgctg ctgaaggccc gcgaggagtt caaggacacc
     3181 gtggacctcc aggtggtcgc cttcccgcag gacggcgtgg tgcgcgaccc gggcgcggac
     3241 gggtacatta gatcggcgct cgagctggga gccgacgtcg tgggcggcat tccgtggatc
     3301 gaatacaccg acgccgacat gcaggcgcac atcgactcga tgttcgccct cgccaaggag
     3361 ttcggcaagg acgtctccat gctgatcgac gacgcgggcg actccgggct ccgctcgctc
     3421 gaaatgctcg ccgtcaagac gatcaaggag gggtggcagg ggcgcgtgac cgcgcagcac
     3481 tgccgcgcca tggccatgta ccaggagccc tacttccgca aggtgctcgc cctgctgcag
     3541 aaagcgaaga taggcctcgt cagcgacccg cacaccggcc cgctccacgc gcgcgtgcgc
     3601 gacctgtacg acgcgggcgt ggccgtcgcg ctggggcagg acgacatcgc cgacgcctac
     3661 taccccttcg gccggaacaa catgctggag atagccttcc tcgccgtcca cctgctctgg
     3721 atgaccacgt tcagcgacat ggagatcctc tacgacctga tcaccacgaa cgcggcgaag
     3781 gccatgggga tcaaggggca cgtcctcgag cccggcggcg ccgccgatct cgtcgtactc
     3841 aacgcggagg acgtctacca cgccatctgg gagcacgagg cgcccctcca cgtcatccgc
     3901 aagggcaagg acgtcacgct gaagtaggcg ccgcaagaga tcgcaagaaa gcacgccgcc
     3961 cccggagaag atttccggga gcggcgcgtt gtcgatcata tcaatgtata tcaaggaaca
     4021 tccaaaaggg catccgggat gcaaaccggc tg
//
LOCUS       DAOSWW010000121         4042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15377_length_4042_cov_3.144720, whole genome shotgun sequence.
ACCESSION   DAOSWW010000121 DAOSWW010000000
VERSION     DAOSWW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15377_length_4042_cov_3.144720"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="PK442_03630"
     CDS             complement(<1..1833)
                     /locus_tag="PK442_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO86769.1"
                     /translation="MKKNIKVTLNGKTVYGYEGQRILDLCTECGVEIPTLCYDPHLSL
                     HGGCSVCLVEVEGAKTLLRACANTISQGMIIKTETERAVSARRTALELLLSDHVGDCR
                     PPCTLTCPANGNVQAYINLAAQGKYDESLDVLHHHVTLPACIGRVCPAPCEKKCRRNF
                     VDDAPVSIREIKRFVGDWAIRNEKMGFIPEIEENGKRIAIVGGGPAGISAAYYLRLKG
                     YAPVIFEKEMLLGGMMRYGIPDYRLPQQVLQAEIDWLLAHGVEVRTGMTLGENITLEA
                     LRKEFDGVILAMGCWKSSPMRVPGEDLEGVLGGINFLYDVKTNPSVTIGRRVAVVGGG
                     NTAMDACRSARRLGAEQVSVLYRRSREEMPADNIEIEEATEEGVDFIYLAAPKAIEGN
                     GKVERILCERMQLGEPDASGRRSPVPTGETFILEVDTVIAAVGQGIDFSTVPAELHDG
                     RKMKVGKDYDTPLPGVFVCGDQQTGPKIAIEAIGNGHWAADSLDHYLTHGTPKKPFFY
                     DIVQTDLGPEDFADVERTTQEHVAHVSGETRLAAPFKEYSKGLTEEQTLRDAKRCMEC
                     GCPDVFECKLRKFATTHEVQPEKLAGAHIEKFEDANQYYVRNMDK"
     gene            complement(1861..3627)
                     /locus_tag="PK442_03635"
     CDS             complement(1861..3627)
                     /locus_tag="PK442_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOO86770.1"
                     /translation="MTKTIVKVGLASCGVAAGAVPIYETLLSALKGHDDVEIKKVGCI
                     GLCYMEPIVEIERDGKSIAYGKVDTELAKEIAESHALRGEIVQRAVILDPSSKACENE
                     RMDSQVRIVLRNSGLIDPEKIDEYVARDGYGALKKALSMTPEQVIEEVLASGLRGRGG
                     AGFPTGLKWKFARGAKGDKKYFVCNADEGDPGAFMDRSVLESDPHSIIEGMAIGGYSI
                     GSDEGIIYCRAEYPLAIKHLNVALEQAREKGYLGKNILGSNFSFDIHIKEGAGAFVCG
                     EETAMIASIEGRRGMPRPRPPFPANSGVFGKPTNINNVETIANIPWIILNGAAKFSEY
                     GIGKSRGTKVFALAGKIKKGGLAEVPMGMPLREVIFNVAGGIAENKKFKAVQLGGPSG
                     GCIPESLLDTPVDYESINATGAIMGSGGMVVMDETTCIVDVAKFFLAFVQKESCGKCP
                     FCRIGTKRMLEILERISEGKGVMEDLDTLLDLAYQVKEGSLCGLGQTAPNPVLTTLKY
                     FREEYEAHIRDKKCPAKVCTSLIHYVIDPAVCIGCTKCARNCPVSCIAGEVKKPHVID
                     DEKCIRCGQCKKVCPVGAISVE"
     gene            complement(3646..>4042)
                     /locus_tag="PK442_03640"
     CDS             complement(3646..>4042)
                     /locus_tag="PK442_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOO86771.1"
                     /translation="EMGYIAPEAVDFIAHELDIPAAEMFGVATFYAMFRLQPEGKHVI
                     RLCRGTACHVQGSARIGEQIQRHLSVTDGGTTSDGVFTLQFVACLGCCSLAPVMMVDE
                     EVFGLLTPEKAVDVLESVRSASSGKGAAD"
BASE COUNT          732 a         1225 c         1170 g          915 t
ORIGIN      
        1 cttgtccatg ttccgtacgt agtactggtt ggcgtcctcg aacttctcga tgtgcgcgcc
       61 cgccagcttc tcgggctgta cttcatgcgt agtcgcgaac ttgcggagct tgcattcgaa
      121 gacgtcgggg cagccgcact ccatgcagcg cttcgcgtcg cggagggtct gttcttcggt
      181 cagcccttta ctgtactcct tgaagggggc ggccagccgt gtttctcctg atacgtgcgc
      241 gacgtgctcc tgggttgtgc gttcgacgtc ggcgaagtcc tcggggccga ggtccgtctg
      301 gacgatatcg tagaagaacg gtttcttggg cgttccgtgc gtcaggtagt ggtccagcga
      361 atccgccgcc caatgtccgt tgccgatcgc ctcgatggcg atcttgggtc ccgtctgctg
      421 gtcgccgcag acgaagacgc cgggaagcgg cgtatcatag tctttgccga ccttcatttt
      481 tctgccgtcg tgcagctctg ccggaacagt ggagaagtcg attccctgac ctactgccgc
      541 gatgacggtg tcgacctcga gaataaaggt ctcgcccgtg ggaacggggc tccgtctacc
      601 ggaggcgtcg ggttcgccga gttgcatgcg ttcgcagaga attcgctcga cctttccgtt
      661 tccttcgatt gccttcggcg cggcgagata gatgaagtcg accccttctt cagtagcctc
      721 ttctatttct atgttgtcgg ccggcatctc ctcgcgggag cgccggtaga gcacggagac
      781 ctgctccgcg ccaaggcggc gggccgaacg gcatgcgtcc atcgcggtgt ttccgccgcc
      841 gacgaccgcg actcttctgc cgattgtgac ggagggattt gtcttcacat cgtacaggaa
      901 gttgatgcct ccgagtacgc cttcaagatc ctctcccgga acgcgcatgg gggaggactt
      961 ccagcacccc atggcgagga tcacgccgtc gaactccttc cggagcgctt cgagtgtgat
     1021 attttcgccg agggtcatcc ctgtgcgcac ctccacgccg tgcgcgagca gccagtcgat
     1081 ctccgcctgg aggacctgct gcggcaggcg gtagtcgggg atcccgtacc gcatcatacc
     1141 gccgaggagc atctcctttt cgaagatgac cggggcgtac cccttcagcc gaagatagta
     1201 agcggcggaa ataccggcgg ggcctccgcc cacgatagcg attctctttc cgttttcttc
     1261 gatctcggga atgaatccca tcttctcgtt tctgatcgcc cagtcgccga cgaatcgttt
     1321 gatttcccgt attgagacag gcgcgtcgtc gacaaagttt ctacggcatt tcttctcgca
     1381 gggggcgggg cagaccctgc cgatgcacgc agggagcgtg acatggtgat ggagcacgtc
     1441 gagtgactcg tcgtactttc cctgcgcggc gagattgata taggcctgca cgttgccgtt
     1501 cgcagggcag gtgagcgtac atggaggacg gcagtccccc acgtggtcgg agagcagcag
     1561 ctcgagtgcg gtacggcgcg cgctgactgc acgctcagtc tcggttttta tgatcattcc
     1621 ctgcgagatg gtgttggcgc acgcgcgcag gagcgtcttc gcaccttcga cttcgacgag
     1681 acagacagaa cagcctccgt gcaggctcag atgcggatcg tagcagagcg tgggaatctc
     1741 cacgccgcac tcggtacaga ggtcgagaat ccgttgcccc tcgtagccgt agacagtttt
     1801 tccgttgagt gtgactttta tgtttttctt catggcagct cgaaatcctc ccgacaatgc
     1861 ttattccacg gaaatggcgc ccacagggca gaccttttta cactgaccgc accggatgca
     1921 cttttcgtcg tcgataacgt ggggcttttt tacttctccg gcgatacagg agacaggaca
     1981 gttccgggcg cactttgtgc agccgatgca cacggcgggg tcgatcacgt agtgaatgag
     2041 actcgtacag accttcgcgg ggcacttctt gtccctgatg tgtgcctcgt actcttcgcg
     2101 gaagtatttc agcgtggtaa gcacggggtt cggagctgtc tggccaagac cgcagagaga
     2161 gccctccttt acctgatagg caagatcaag gagcgtatca aggtcttcca tgacgccctt
     2221 cccctcggag atgcgttcca ggatctcaag catccgcttc gtcccgatcc ggcagaaggg
     2281 gcacttcccg caggactcct tctgaacgaa ggcgaggaag aacttggcaa cgtcgacgat
     2341 gcaggtcgtt tcgtccatga ccaccattcc gccggagccc atgatggcgc ccgtggcatt
     2401 gatggattca tagtcaacag gggtgtcgag cagcgattcg ggaatacagc ctcccgaagg
     2461 acctccaagc tggacggcct tgaacttctt gttttctgcg ataccgcccg ccacgttgaa
     2521 gatgacctcg cgcagcggca ttcccatcgg aacctccgcg aggccgcctt tcttgatttt
     2581 tcctgcgagg gcgaagactt ttgtcccccg gctcttgccg attccgtatt cggagaactt
     2641 cgccgctccg ttcagaatga tccagggaat attcgcgatg gtctccacgt tgttgatatt
     2701 cgtcggtttg ccgaagacgc cgctgttcgc agggaacgga ggccgcggcc gcggcatgcc
     2761 gcgccggcct tcgatggaag cgatcatggc agtctcctcg ccgcatacaa aggcgcccgc
     2821 gccttctttt atgtgaatgt cgaaggagaa gttcgacccc aaaatgttct tcccgaggta
     2881 ccctttttca cgcgcctgtt cgagcgcgac gttcaggtgc ttgatggcga gcggatactc
     2941 cgctctgcag tagatgatcc cctcgtccga accgatggag tagccgccga tggccattcc
     3001 ctcgatgatg ctgtgcggat cgctctccag aacggagcgg tccatgaacg cgccggggtc
     3061 gccctcgtcg gcgttgcaga cgaagtattt tttgtccccc ttggcgccgc gagcgaactt
     3121 ccatttgagt cctgtgggga atcccgcgcc gccgcgcccg cggaggccgc tggcgagaac
     3181 ctcttcgatt acctgctccg gcgtcatgga cagcgccttt ttcaatgcgc cgtagccgtc
     3241 gcgggcgacg tattcgtcga tcttttccgg gtcgatgagt ccgctgttcc tgagaacgat
     3301 acgtacctga ctgtccatac gctcgttttc acaggctttg gatgaaggat cgagaatcac
     3361 cgcacgctga acaatctctc cgcgaagcgc gtgcgactcg gcgatctcct tcgcgagctc
     3421 ggtatccacc ttaccgtagg cgatggattt cccatcacgt tcaatttcaa cgataggctc
     3481 catgtagcag agtcctatgc agccgacttt ttttatctcg acgtcgtcgt gtcctttcag
     3541 ggccgagaga agagtttcat atatcgggac ggcgccggca gccacgccgc agctggccag
     3601 accgaccttc acgatggttt tggtcacctt gcacacctcc atgagctagt ccgcagcgcc
     3661 ttttcccgag gaggcgctgc gcacggattc aagaacatcg acggcttttt cgggcgtgag
     3721 cagcccgaag acttcctcgt ccaccatcat aaccggcgcc agactgcaac atcccagaca
     3781 ggcgacgaac tgaagcgtaa acactccgtc ggaggttgtc ccgccgtcgg tcaccgaaag
     3841 atgccgctga atttgctctc ctatacgcgc cgatccctga acgtgacatg ccgtcccccg
     3901 gcaaaggcgg atcacatgct ttccctccgg ctgcaggcgg aacatggcgt aaaatgtggc
     3961 gacgccgaac atctccgcag caggaatatc cagctcgtgt gcaataaaat ccaccgcttc
     4021 cggagcgatg taccccatct cc
//
LOCUS       DAOSWW010000122         4028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15456_length_4028_cov_3.315631, whole genome shotgun sequence.
ACCESSION   DAOSWW010000122 DAOSWW010000000
VERSION     DAOSWW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15456_length_4028_cov_3.315631"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /gene="fliD"
                     /locus_tag="PK442_03645"
     CDS             <1..1169
                     /gene="fliD"
                     /locus_tag="PK442_03645"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOO86772.1"
                     /translation="AFTLSGDTSILSFLQLDGSNTTDPLVTAQDAKFKIDGLTVTRSS
                     NQVDDLLEGVKLTINGPGTVIMDITQDAEQAVTGVQDFVTAYNDALDWINIRLSESTQ
                     EDADDDFSKKFGLLHGNSMLWQSKSQLRMLMTSSVTAKYSTKTGSTVLGTMASRGLSS
                     NSTFELTVGVRTAQIEVTPSDTLSTIASKINTSYEMLHDEQGRTYPIPMASAKVVNNQ
                     LVIEASPGRKFSLSASDDVLDTLGLGTPFTLLSQLGISTESADYGKSGKLEFDTDKFM
                     EALREDPEGVAAIMNTMMTSMDDYIGNMVDTSQVQVGDTTAPKGRIASQIYTFRSEIT
                     TLDKRISDLERRLEVRARGLYESFANAEVRLAKLQQQASWLSSTLSQLTGGSGN"
     gene            complement(1280..1966)
                     /locus_tag="PK442_03650"
     CDS             complement(1280..1966)
                     /locus_tag="PK442_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO86773.1"
                     /translation="MEQIQVLIADDHKLFRDGVKKLLELESDIVVIGEAGDGEEALLL
                     IREHEPDILLFDINMPRVDGIQLVRELNNIPHKMRYVAISAYDDEDCLSALSAAGVLG
                     FVLKASGKVELLSAIRSAARGQPYVDPRVAGKLLTSFSRRKEENDQLYELTPREKEIL
                     YWLSQGLSNTEVADRMVLSEKTVKNHVSHVLKKLDLRDRTQAAILAWRIGFAQLSPDT
                     LAHILESEVR"
     gene            2112..2912
                     /locus_tag="PK442_03655"
     CDS             2112..2912
                     /locus_tag="PK442_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86774.1"
                     /translation="MTEKNTSTLPFSIRKKMGLAITEFQMITSGDRIMIGLSGGKDSL
                     MLATTLSGLRRRSPVKFSLSACTVDITGGETNTESLRAFCESLDISYSVKTHPIVNII
                     NIRNERSPCSLCANIRRGILNAAVNESGCNVLALGHNLDDAVETALMNLFHTGRFKSF
                     QPKFWQSRSGVTVIRPLILVEERRILKEVSRLELPVLPYICPFSLETERARAKKIIDQ
                     LAAGNPRIKYNIIHALKSIDEGDRWELSAHRKKQEHSPGSGSFVPILD"
     gene            3002..>4028
                     /locus_tag="PK442_03660"
     CDS             3002..>4028
                     /locus_tag="PK442_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOO86775.1"
                     /translation="MGKNEEYSVQLPPSGQFSPSEKSASFTENEEIVRRYFRWEPLDD
                     PSFAHLIVGKGSIGGKGRSLLFAMAKLRDSGVETLKRVVFPPSLFIATDSFDSVVSQI
                     PNLEALKKGSPETIEEAFLATKLPDSVLGAIRKFLEKITDPIVVRSSSVLEDSLKYSF
                     AGKYLSTFEFNGGDESLEERAAHVESDIKKIYARTFFPVAVAYRQKHGLGDDRMGIIL
                     MRVAGKWRGRYYYPTMAGVGFSRYYRRWTTRIKSEDGIIRLVFGMGTTSTKRGYARTF
                     ALTLPSLRPEGQNPFNIMRHSQEHFQVIDREQKALVTVDVKQIWKDIFPYHSCFPDYA
                     QIYSPDEE"
BASE COUNT          991 a         1117 c         1044 g          876 t
ORIGIN      
        1 atgcgttcac tctttccggc gatacgagta ttcttagttt tcttcagctc gacggcagta
       61 atacgacaga tcctttggtt accgctcaag acgcgaaatt caagattgac ggtctcacag
      121 taactcgctc ctcgaaccaa gtcgacgacc tccttgaagg agtcaaactg acgatcaacg
      181 gcccgggaac ggtcattatg gacattaccc aggatgcgga gcaagccgtg accggagtac
      241 aggattttgt gactgcgtac aacgacgctc tcgactggat caacatccgc ttatcggaga
      301 gcacgcagga agatgccgac gacgacttct caaagaagtt cggattgttg cacggcaact
      361 ccatgctctg gcagagcaaa tcgcagcttc gaatgcttat gacaagctcg gtaacggcga
      421 agtactcgac caagaccgga agcacggttc tcgggaccat ggcttcgagg ggattgagtt
      481 cgaattccac gttcgagctc acagtgggcg tgcgaacagc ccaaatagaa gtcacgcctt
      541 cagatactct gagcacgatc gcaagcaaga tcaatacttc ctacgaaatg ctccacgacg
      601 agcaggggcg tacgtacccc attccgatgg ccagcgcaaa agttgtgaac aaccagctgg
      661 tgatcgaagc gtcgccggga agaaaattct ccctctccgc ctccgacgac gtgcttgaca
      721 ctctcggttt gggcacgccc tttacgcttt tgtcccaact tggtatctcc accgagagcg
      781 cggattacgg caagagcgga aagctcgaat tcgataccga caagttcatg gaagcgctac
      841 gggaggatcc ggaaggagtt gccgccatca tgaacaccat gatgacgagc atggatgact
      901 atatcggcaa catggtggac acaagccagg tgcaggttgg cgacacgact gcacccaagg
      961 gacggattgc cagccagata tacacctttc ggtcggaaat tactactctt gacaaacgta
     1021 tttcagacct ggagcgccgg cttgaagtac gggcacgcgg gctctacgag tcgttcgcaa
     1081 acgccgaggt tcgtcttgcg aagctccagc agcaggcaag ctggctctcc tccacattga
     1141 gccagctgac cggcggttcc ggcaactaaa atagatactg aggcttttcg tttcctctac
     1201 acaaaacagg gttccgcggt accaactgcg gagccctgtt ttttcgttct cttgaggcat
     1261 aaggaaacag atttgattcc tatcgcactt cgctctccaa aatatgcgcc agcgtatcag
     1321 gagaaagctg cgcaaagccg atacgccacg cgaggatagc cgcctgcgtc ctgtcgcgga
     1381 gatcgagctt cttgagcaca tggctcacat gattcttcac cgttttctcg gagagaacca
     1441 ttctgtcggc cacctcggta ttgctcaagc cctgagagag ccagtagagg atctccttct
     1501 cgcggggagt cagctcgtac agctggtcgt tttcctcttt cctgcgtgag aatgaggtga
     1561 gcagcttccc ggcgacgcgc ggatcgacgt acggctggcc tctggcggcg gagcgaattg
     1621 cggagagcag ctctaccttt ccggaggctt ttaagacaaa cccaagaacg cccgccgccg
     1681 aaagagcgga aagacagtct tcgtcgtcgt aggcgctgat cgcaacgtac cgcattttat
     1741 gcgggatgtt gttcaattcg cgtacaagct ggatgccgtc cactctgggc atattaatgt
     1801 cgaagagcag gatgtccggt tcgtgctccc tgatgagaag aagcgcttct tctccgtcgc
     1861 cggcctctcc tatcacaacg atatccgact ccagctccag aagcttttta acaccgtcgc
     1921 ggaataactt gtgatcatcc gcaataagaa cctgtatctg ctccatgtgg acacctccgt
     1981 ccgagtctgc gaaaacgcgc tccgttatgc tatagtgaag aaaggtacgc gcggtcccgt
     2041 gccgaatccc tagcatggga cttaagaact attctaacac gatatcgacc gtttttcgag
     2101 gaggaacaca ggtgaccgaa aaaaatacat ccactcttcc tttttcgatc cgaaaaaaga
     2161 tggggcttgc cattaccgag ttccagatga taacctccgg agacagaatc atgatcggtc
     2221 tctccggagg gaaggacagc ttgatgcttg cgacgacgct ctccggattg cggcgaagaa
     2281 gccccgtcaa gttttccctc tccgcctgta ctgtggatat caccggagga gagacgaata
     2341 cagaatcttt gcgagcgttc tgtgaatccc tcgacatttc gtactcggta aaaacacatc
     2401 ccattgtcaa tattatcaat atcagaaacg agcgttcacc ttgcagcctg tgcgcgaaca
     2461 tccgcagagg aattttgaac gcggcggtga acgagtccgg gtgtaatgtg ctcgctctgg
     2521 gtcacaacct tgacgacgcc gtcgaaactg cacttatgaa tctcttccac accggcagat
     2581 tcaagtcttt tcagccgaaa ttctggcaaa gccgctccgg cgtcacggtc atccgcccgc
     2641 tcatcctggt agaagagcgg cgaatcttga aggaagtctc gcggctggaa ctcccggttc
     2701 ttccctacat ctgccccttt tctctggaga ccgaaagagc gcgggcgaaa aaaatcatcg
     2761 atcaacttgc tgccggaaat ccaagaatca agtacaatat aatccacgca ctcaagtcca
     2821 tcgacgaagg agaccgatgg gaactttctg ctcatcggaa gaaacaggaa cattctcccg
     2881 gctccggttc ttttgttccg attcttgatt aaacaggtct tcagcctccg gcatgtcgaa
     2941 accggatgaa cctggtcgct cgcgtctcaa ggagcgacaa tgctcttcaa ggagagatga
     3001 catgggaaaa aatgaagaat actcggtaca gcttccgcca tcgggacagt tctccccatc
     3061 agagaagagc gcgtcgttta cggagaacga agaaattgtc aggagatact tccggtggga
     3121 gcctttggac gatccttcgt tcgcacacct gatcgtcggc aagggcagta tcggcggtaa
     3181 agggcgatcc cttctttttg caatggccaa actccgggac agcggcgttg agacgctgaa
     3241 gcgggtggtc tttccgccgt ctctttttat tgccaccgat tctttcgaca gcgtggtctc
     3301 gcagatcccg aaccttgaag cactgaagaa aggatcgcct gaaactatcg aagaagcgtt
     3361 tctggcgacg aagcttcctg acagcgtact cggcgcgatc agaaaattcc tcgaaaaaat
     3421 caccgatcct atcgttgttc gcagcagcag cgtcctggag gattctctca aatactcctt
     3481 cgccgggaaa tacctctcga cgttcgaatt caacggaggg gacgaatctc tcgaagaacg
     3541 agccgctcat gtagaaagcg acatcaaaaa aatttacgcg cgcacgtttt tcccggttgc
     3601 ggtcgcctat cgccaaaagc acggactggg cgacgacaga atgggaatta tcctgatgcg
     3661 cgtcgccggc aagtggcgcg gccgctacta ctacccgacc atggcgggcg ttggtttctc
     3721 ccggtactac cgacgatgga ccacgaggat taaatccgaa gacggcatca tccgcctcgt
     3781 cttcggcatg ggaaccacca gcacgaaacg agggtacgcc cgcacgttcg cactgacgct
     3841 cccttcgctc cgtccggagg gtcagaatcc tttcaacatc atgcggcact cccaggaaca
     3901 cttccaggtg atagacagag aacagaaggc gcttgtcaca gtggacgtca aacagatatg
     3961 gaaggatatc ttcccttacc acagctgttt ccccgactac gcgcagatct acagcccgga
     4021 tgaggagg
//
LOCUS       DAOSWW010000123         4009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15557_length_4009_cov_4.495448, whole genome shotgun sequence.
ACCESSION   DAOSWW010000123 DAOSWW010000000
VERSION     DAOSWW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15557_length_4009_cov_4.495448"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK442_03665"
     CDS             complement(<1..225)
                     /locus_tag="PK442_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86776.1"
                     /translation="MTLQHVGKSVPRVDAVRKATGEAVFVADIVLPRMLHAKVLRAGV
                     PHAKILSIDTSKAEAMPGVKKVVTGQGCGML"
     gene            complement(377..1321)
                     /locus_tag="PK442_03670"
     CDS             complement(377..1321)
                     /locus_tag="PK442_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO86777.1"
                     /translation="MEQVMTTAFITGLLAAMMRMATPIIFGTLGEILCERAGVLNLGI
                     EGIMLMGAMTGFLTTLTTGSLWLGVAAAALVGVLLSLFMAFLAVYLGLSQHVSGLGIT
                     LFSTGLAMFIYRLAVGSPVNPPTVRPFTQTAIPFLSGIPILGTALFNQYVLVYIALLL
                     VPLIWFLLYRTSWGLAIRTVGENPFAADTVGINVNLTRTLCLAAGGALMGVGGAFLTL
                     AHQNMFLIDVVGGRGWIAIAMVIFGNWDPAKGMAGALIFGFLDALQLRLQGLGFDIPF
                     HLFLIIPYLMTVIALVGVSRRAAAPAGLLKPYRREEKG"
     gene            complement(1321..2373)
                     /locus_tag="PK442_03675"
     CDS             complement(1321..2373)
                     /locus_tag="PK442_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO86778.1"
                     /translation="MIVSKRAPLPGWIQLLVPVGAVLATLLLSAIPILIAGGDLWLSY
                     ASLFKGALGTRYNFLETCVKAAPLTFTGLAVAFAFRAKFWNIGAEGQLLAGAIAAAWV
                     GIYAPPLPKALVLLLVCAAGFLAGGAWATIPALLKTKYRVDDVVTTLLLNYVMWHLMG
                     YLLFGPLQMPGSSWPRSPAVAEIARFPVLLIRSRFHLGIVIALVAVLVVWFVNSKTVF
                     GYQSRAVGVNPRAAAFGGINVNAVIMKTALLSGGLAGLAGVGEVVAIHFHLLMDISPG
                     FGYSGIVIAMLGRLHPVGTVLSAFFFSVIIVGAQSMSRLTGVPTYIAEVIQGMALIVM
                     LIALLLTEYRVKAVRD"
     gene            complement(2370..3914)
                     /locus_tag="PK442_03680"
     CDS             complement(2370..3914)
                     /locus_tag="PK442_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO86779.1"
                     /translation="MSRPDTPRTHLEERTPVVEMRSIRKTFLDVVANSDVDFTLYKGE
                     ICALLGENGAGKTTLMNILFGYYAADSGEIRIGGEKVSLSSPRDAITRRIGMVHQHFT
                     LVPSQTVLENTVVGSCGGFFLDMGKARRKLLELQRRFGLHVDPDAPVWTLPIGGQQKV
                     EILKALYRDARILILDEPTAVLAPLETMELFGTLRALAAEGCSVVFISHKLYEVMEIA
                     DRVVVLSKGSVAAERETRDTDERELANLMVGRELAVQRHSGSQRRGDPALEIRSLSVR
                     NDKELEAVKELSLAIRSGEILGMAGVSGNGQRELAEALFGLRVPDGGDVLLGGEPLPP
                     GRPKTAVDRKMARIPEDRMTTGLLLELTVEENLVLENHASFRSFGMLDHAAIGRHADR
                     LIGEFGITTDGRRAVARTLSGGNLQKIILARELSASPRAVVAAQPTRGLDVGAIEYVH
                     KRILEARESGAAILLISEDLDEIFLLADRIAVMYEGRIMGVAERDEASREQVGLWMSG
                     VNEPCA"
BASE COUNT          733 a         1325 c         1318 g          633 t
ORIGIN      
        1 gagcattccg cacccctgcc ctgtgacgac tttcttgacg ccgggcatcg cctccgcctt
       61 cgacgtgtcg atcgagagaa ttttggcgtg agggaccccg gccctcagca ccttcgcgtg
      121 gagcatccgc ggcagaacga tgtcggccac gaagacggcc tctcccgtcg ccttccggac
      181 cgcgtccacg cgcggaacgc tctttcctac atgctgtaac gtcatgcttt cccctctctt
      241 tcacaaagag atgcgatgaa aaagttgctc cgaaatggag cgagcccgaa aagtgcgcag
      301 ggcgccgccc cccggaagta gtgacgccgg acacgcacgg gcagaaggaa cgcgcccgtg
      361 ctccccggag tttcctctac cccttctcct ccctccggta cggcttcagc agccccgccg
      421 gagccgccgc gcgccgcgaa acgccgacga gcgcgatgac cgtcatcagg taggggatga
      481 tcaggaagag gtggaacgga atgtcgaagc ccagcccctg gaggcgcagc tggagcgcgt
      541 cgaggaagcc gaagatcaac gcgcccgcca tccccttcgc cgggtcccag ttgccgaaga
      601 tcaccatggc gatggcgatc caccccctgc cgcccaccac gtcgatcagg aacatgttct
      661 ggtgggccag ggtcaggaac gcgccgccga cgcccatgag cgcgccgccc gccgcgaggc
      721 agagcgtccg cgtcaggttg acgttaatgc ctaccgtgtc cgcggcgaag gggttttccc
      781 cgacggtgcg gatggcgagc ccccacgagg tgcggtagag caggaaccag atcagcggca
      841 cgaggagcag cgcgatatag accagaacgt actggttgaa gagagccgtc cccaggatgg
      901 gaatgcccga gaggaacgga atcgccgtct gcgtgaaggg gcgcaccgtg ggcgggttca
      961 ccggggagcc gaccgcgagc ctgtagatga acatggccag ccctgtcgag aagagcgtga
     1021 tcccgagccc cgagacgtgc tgcgagagcc ccaggtagac ggcgaggaag gccatgaaga
     1081 gggagagcag cacgccgacg agcgccgcgg ccgctacgcc gagccagagc gagcccgtgg
     1141 tcagcgtagt gagaaagccc gtcatcgccc ccatcagcat gattccctcg ataccgaggt
     1201 tgagcacccc ggcgcgttcg cagaggatct ccccaagcgt gccgaagatg atgggcgtcg
     1261 ccatgcgcat catggccgcc agaagtcccg tgataaaagc ggtggtcatc acctgttcca
     1321 tcagtctctc accgccttta cgcggtattc ggtcagcagc agggcgatga gcatcacgat
     1381 gagcgccatt ccctgtatca cctccgcgat gtaggtcggg acgccggtca ggcggctcat
     1441 cgactgcgcc cccacgatga tcacgctgaa gaagaacgcg gagaggacgg ttccgaccgg
     1501 gtgcagccgc ccgagcatgg cgatgacgat gccggagtag ccgaagccgg gggagatgtc
     1561 catcagcagg tggaaatgga tcgcgaccac ctcgcccacg cccgcgagcc ccgcgagccc
     1621 cccggagagg agcgcggtct tcatgatgac ggcgttcacg ttgatgccgc cgaaggccgc
     1681 cgccctcggg ttcaccccga cggcgcgcga ctggtagccg aagaccgtct tgctgttcac
     1741 gaaccagacg acgagcacgg cgacgagcgc gatgacgatg cccagatgga agcgcgagcg
     1801 gatcagcagc accgggaagc gcgcgatctc cgccacggcg ggggagcggg gccagctcga
     1861 ccccggcatc tgcagcgggc cgaagagcag gtagcccatc aggtgccaca tgacgtagtt
     1921 cagcagcagc gtggtgacca cgtcgtccac ccggtacttc gtcttgagca gcgcggggat
     1981 cgtcgcccac gctcctccgg ccaggaatcc ggcggcgcag acgagcagca ggacgagcgc
     2041 tttcggaagg ggcggcgcgt agatgccgac ccaagcggcg gcgatggctc ctgcgaggag
     2101 ctgtccttcc gccccgatgt tccagaactt cgcccggaag gcgaaggcca cggcaagccc
     2161 ggtgaaggtc agcggcgccg ccttcacgca ggtctccagg aagttgtagc gcgttcccag
     2221 cgctcccttg aaaagggagg cgtacgagag ccacaggtcg cctcccgcga tcagaatggg
     2281 gatagccgag agaagcagcg tcgccaggac ggcccccacc ggcacgagaa gctgtatcca
     2341 tccgggcaga ggcgcccgct tcgacacgat catgcgcacg gttcgttcac cccgctcatc
     2401 cagagaccga cctgctctct gctcgcttca tcccgctcgg cgacgcccat gatacgccct
     2461 tcgtacatca cggcgatccg gtcggcgagc aggaagattt cgtcaaggtc ctccgagatc
     2521 aggaggatgg ccgcgccgct ctcgcgggct tccagaatcc gcttgtggac gtactcgatg
     2581 gccccgacgt cgagtccgcg cgtcggctgg gccgcaacga cggcgcgcgg ggaggccgat
     2641 agttcgcggg cgaggatgat cttctgcagg ttgcccccgg agagcgtccg ggcgacggcg
     2701 cgccgtccgt cggtcgtgat gccgaactcg ccgatcagcc tgtccgcgtg cctgccgatc
     2761 gccgcgtggt cgagcatgcc gaacgagcgg aaggaggcgt ggttttcgag gacgaggttc
     2821 tcctccacgg tgagttcgag cagcagtccc gtggtcatcc tgtcctccgg gatacgggcc
     2881 atcttccggt ccactgcggt cttcggcctc ccgggaggaa gcggttctcc gccgagaagc
     2941 acgtcgccgc cgtcggggac gcggaggccg aagagggctt ccgcaagctc ccgctggccg
     3001 ttgccggaga ccccggccat cccgagaatc tccccggaac ggattgcgag ggagagctcc
     3061 ttgaccgcct ccagctcctt gtcgttcctt accgagagcg agcggatctc gagcgcgggg
     3121 tcgccccgcc gctgcgagcc gctgtgccgc tgtacggcga gttcccgtcc caccatcagg
     3181 ttcgccagct cgcgctcgtc cgtgtcgcga gtctcccttt cggcggccac gctccccttc
     3241 gagagaacga ccaccctgtc ggcgatctcc atgacctcgt agagcttgtg cgaaatgaag
     3301 acgaccgagc acccctcggc ggccagtgcg cgcagcgtgc cgaagagttc catcgtctca
     3361 agaggagcga gcacggccgt cggctcgtcg aggatgagaa tgcgcgcgtc gcggtagagc
     3421 gccttcagga tctccacctt ctgctggccg ccgatgggga gggtccacac gggcgcgtcc
     3481 gggtcgacgt gcaggccgaa tcggcgctgc agttcgagca gcttccggcg cgctttcccc
     3541 atgtcgagaa agaagccgcc gcagctcccc acaacggtgt tttcaagcac tgtctgcgaa
     3601 gggacgaggg tgaagtgctg gtgcaccata ccgatgcgcc gggttatggc gtcgcggggg
     3661 gaggagaggg agaccttctc gcccccgatg cggatctcgc cgctgtccgc ggcgtagtat
     3721 ccgaagagga tgttcatgag cgtcgtcttc cccgcgccgt tttcgccgag aagggcgcag
     3781 atctcaccct tgtacagagt gaagtccacg tcgctgttcg cgacgacgtc gagaaaggtc
     3841 ttccggatcg agcgcatctc aacgaccggg gtgcgctctt caaggtgtgt gcgtggtgtg
     3901 tcgggccggc tcatcggcag ccattccccc tttcgggtgt ttccgtcgaa aaggggaacg
     3961 gaggcatccc cccgttcccc aaaaaaacat cagtccgtct tcaattccg
//
LOCUS       DAOSWW010000124         3985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15684_length_3985_cov_3.275827, whole genome shotgun sequence.
ACCESSION   DAOSWW010000124 DAOSWW010000000
VERSION     DAOSWW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15684_length_3985_cov_3.275827"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PK442_03685"
     CDS             complement(<1..668)
                     /locus_tag="PK442_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO86780.1"
                     /translation="MYCTLKERYRLRGWEQLPYAIQDVASGGVVFLDEKAFQAASFCN
                     GMVDVASPLLLPAHRRAVSLLKEQGVVEECAFGEGLKEEQKYRLIPCRYMRKVHWSIT
                     GRCNLKCRHCFMSAPHAKYGELSLEQCLNIVRQISEAGILDVSLTGGEPLVRSDFFEI
                     VDALLDERIMISQVYTNGVLVDEALLNKFEARGIRPEFLLSFDGIGCHDWLRGVPGTE
                     RQTI"
     gene            1054..2298
                     /locus_tag="PK442_03690"
     CDS             1054..2298
                     /locus_tag="PK442_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO86781.1"
                     /translation="MKKRPLLYVVFLLPLILPCAAFSAHMDEMQYLALVMERNNDLAA
                     ARQEIEARLFSARAELAIQRPSLSLSAETSKWLDKNRGDQQADLSLSHRVNLSGAYGL
                     QERDLLLGVEILRNQYADAVNERLALAASAYRQAVMAALNREAMEQIVEKRRESLTIT
                     KEKFDKELVSLLELLRAQSQVDDGEAFLLQARQKYEQQLVEMSALAGGAPAIPSVALP
                     ELGGMNLVVDEEKAWDNRPDVAALLLSKERASVQRSLAAKGLSPFVDLSVGLRVLEDH
                     IFPQERGGEVFVRAVLTVPLADGGKTSNSTEAATLMLRKAEYELAARRDSLKREADMV
                     RERWNRALGLTAADGHDFKRADKELEIAELMYREGMGSQLDLINAQEADQKSRTEFLS
                     AVQELWLVLAEADRVMGRRAPR"
     gene            complement(2365..2661)
                     /locus_tag="PK442_03695"
     CDS             complement(2365..2661)
                     /locus_tag="PK442_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HOO86782.1"
                     /translation="MSIESAKAFYERLETDTELQEKLKKLNTKENIEPYVKGELGYEF
                     TLEEMQKVIFEKNPEMTDEELEAVVGGSVIGTIGLILLAGPALFLGLAAGIAAA"
     gene            complement(2717..>3985)
                     /locus_tag="PK442_03700"
     CDS             complement(2717..>3985)
                     /locus_tag="PK442_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HOO86783.1"
                     /translation="ELPYRSLPTWHKEILFALARGVAVVGAAAVGAHRAVELAPNGME
                     GIGGIFERVASGELERDDEVLCDWEETPGGIRRLTLPLVTMRDALSAAKDAGVLSEKS
                     VALLLSYAEGLFWKKRTWKALLAASEDGADGVPQDELRAFESWLPNAPDPVKEDAEAL
                     LRRVEELASGNGESGRTVRNDSAGKGDPGEASALFKSLDQRDRPVPTSNGTIRQWMIG
                     DMVSFAHPEAEDLNGGALNRRLALLLAALWGVVPRSEELEAEEKRLRRRFKLKSDEAF
                     AAWKKENDLDEEEMARLLEDEALLHKLHRWYMQGRIHGKNTGAVLDELKLRGGYAEWK
                     ERAARRDALLHGRPEVLREERERACSTPFMTLVREHLRADGFPWHGAMLPEAIPETGL
                     EPRDLVEELLLARAARRILGETTQFGTGEK"
BASE COUNT          724 a         1364 c         1152 g          745 t
ORIGIN      
        1 tcgatcgtct gcctctccgt cccgggcacg ccgcgcagcc agtcgtggca gccgatgccg
       61 tcgaaactga gcaaaaactc cggacgaatt ccccgggcct cgaacttgtt caggagcgcc
      121 tcgtccacca gcacgccgtt ggtgtagacc tgtgagatca tgatgcgctc atcgagcagg
      181 gcgtccacta tctcgaagaa gtcgcttctg accagcggct cgcctccggt cagcgacacg
      241 tccaggatgc ccgcttcgga aatctgccgg acaatgttca ggcactgctc cagcgacagc
      301 tccccgtact tcgcgtgcgg cgcggacatg aagcagtggc ggcacttcag gttgcatctc
      361 cccgtgatcg accagtggac cttgcgcata tacctgcacg gaatcagcct gtacttttgc
      421 tcctccttca gcccttcgcc gaaggcgcac tcttcgacca ccccctgttc tttcagcagc
      481 gagacggccc ggcgatgcgc ggggagcagc agcggagagg ccacgtccac catgccgttg
      541 cagaaggagg cagcctggaa cgccttctcg tccagaaaga cgacgccgcc actcgcaaca
      601 tcctgaatcg cgtacggcag ctgttcccac cctctgagcc tataccgttc ctttagcgtg
      661 caatacaccg cctcatcctc tcccctcgcg ttccatcatc ttcttcggtt caggtactgg
      721 acgacgaccg aaagatcgcc ccgggacgac gaaagacgga aaggctttgc atcgtcgact
      781 tcagacgcag catacacctt tacacaatgt caaagtcaag cggcattttt caaaagagtc
      841 gcaacctgca ccgaattcta tcatccgacg gagaaaaaag ggggcggttt attttctccc
      901 cctcggaaac gcagtatcga caagtctttg tagaattacc gaaagaatgc tttccattcg
      961 gcccgaatga gcaacgtatg gttttcgacg ggccgctggg gtagaatgat cgcagtgaac
     1021 gactattcat tcgatccatt tcaaggaggg tccatgaaaa aacgcccgct cctctatgta
     1081 gtgtttttgc ttccgctcat tcttccctgc gccgcctttt ccgcgcacat ggacgagatg
     1141 cagtatcttg cgctcgtgat ggagcggaac aacgacctcg ccgccgccag gcaggagatc
     1201 gaggcgcgcc tcttctccgc ccgggcggaa ctggccatcc agcggccgtc gctttcgctc
     1261 tccgccgaga cgagcaagtg gctcgacaag aaccggggcg accagcaggc cgatctctcc
     1321 ctctcacacc gcgtcaacct ctccggagcc tacggcctcc aggagcggga tctgctgctc
     1381 ggcgtcgaga tccttcggaa ccagtacgcc gatgcggtca acgaacgcct cgccctcgcc
     1441 gcgtccgcct accgccaggc ggtgatggcc gcgctgaacc gggaggcgat ggaacagatc
     1501 gtggagaagc gccgcgagtc gctcacgatc accaaggaga agttcgacaa ggagctggtc
     1561 tccctgctgg agctgctgcg ggcgcagtcg caggtagacg acggcgaagc cttcctcctc
     1621 caggcccggc agaagtacga gcagcagctc gtggaaatga gcgctctcgc cggaggggct
     1681 ccggcgattc cgtcggttgc gcttcccgaa ctcggcggaa tgaatctcgt cgtggacgag
     1741 gagaaggcgt gggacaaccg ccccgacgtc gccgccctgc tgctctccaa ggaacgggct
     1801 tcggtgcagc gctcgctcgc cgcaaagggg ctctcaccgt tcgtcgacct ctccgtcggg
     1861 ctgagggttt tggaggacca catttttccc caggagcggg gcggagaagt cttcgttcgc
     1921 gccgttctca cagttcctct tgccgacggc gggaagacat ccaactccac cgaagcggcg
     1981 acgctgatgc tccgtaaggc cgagtacgag ctggccgcaa gacgcgacag tctgaaacgg
     2041 gaggccgaca tggtgcggga gcggtggaac cgcgcgctcg gcctgacggc ggcggatggc
     2101 cacgatttca agcgcgcaga taaagagctc gaaatcgccg agctgatgta ccgcgaggga
     2161 atgggctccc agctcgacct gatcaacgcc caggaagccg accagaagag ccggacggag
     2221 tttttaagcg ccgtgcagga gctctggctc gtcctcgcgg aggccgacag ggtcatgggc
     2281 cgccgggcgc cgcggtaaag ctttcattcc ctccgcactt aagaagtccc gaaggcgcgt
     2341 ccgcctacgg gacttcttaa gtgatcaggc tgctgcaatc ccggcggcaa gccccaggaa
     2401 caacgccgga ccggcaagaa ggatcagccc gatcgtgccg atcaccgagc ccccgacgac
     2461 ggcctcaagc tcctcgtcgg tcatctccgg attcttctcg aagatcacct tctgcatctc
     2521 ctccagcgtg aactcgtacc ccagttcgcc cttcacgtac ggctcgatgt tctccttcgt
     2581 gttcagcttc ttcagcttct cctgaagctc ggtatccgtc tccaagcgtt cgtaaaacgc
     2641 cttcgccgat tctatcgaca tgcacgacac ctccctcgaa ttcgcctctc cctcgcggaa
     2701 ggggcggcag actccgtcac ttctctccgg ttccgaactg cgttgtttcc cccagaatcc
     2761 tccgggcggc gcgggcaagc agcagctcct ccaccaggtc gcgcggttcc agccctgtct
     2821 ccggaatcgc ctccggcagc atcgccccgt gccacgggaa gccgtccgcc cgcagatgtt
     2881 cgcgcaccag cgtcatgaag ggcgtggaac acgcgcgctc acgctcttca cggagcactt
     2941 ctggccgtcc gtgcagcagc gcgtcgcgcc tcgcggcgcg ctccttccac tccgcgtacc
     3001 cgccccggag cttgagctcg tcgagcaccg ctccggtgtt cttgccgtga atacgcccct
     3061 gcatgtacca ccggtggagc ttgtgcagca gcgcctcatc ttcaagaagt cgggccatct
     3121 cctcttcgtc gaggtcgttc tccttcttcc atgcggcgaa cgcttcgtcg ctcttgagtt
     3181 tgaagcggcg gcgaaggcgc ttctcctcgg cctccagctc ctcgcttcgg ggaaccacgc
     3241 cccacagagc ggcgagcagc agggcaagac gccggttcag ggctccgccg ttcaagtcct
     3301 ccgcctcggg gtgggcgaag ctcaccatgt cgccgatcat ccactgacgg atcgtgccgt
     3361 tcgacgtcgg caccgggcgg tcgcgctggt ccagcgactt gaagagcgcg gacgcctcgc
     3421 cgggatcgcc tttccccgcg gaatcattcc ggacagtcct tccggactcc ccgttcccgg
     3481 acgcgagttc ctccacgcgg cgcagcagcg cttcggcgtc ctccttcaca gggtcggggg
     3541 cgttcggcag ccacgattcg aacgcgcgca gctcgtcctg cggaacgccg tccgctccat
     3601 cttccgatgc ggcgaggagc gctttccaag tccgtttctt ccagaagagc ccttcggcat
     3661 acgagagcaa caaagcgacg ctcttctcgg agaggactcc ggcatccttc gccgccgaca
     3721 aggcgtcgcg catcgtcacc agcggcaatg tgagacggcg gattccgccc ggcgtctctt
     3781 cccaatcgca gagcacttca tcgtcgcgct ccagttcgcc ggaggcaacg cgttcaaaga
     3841 tccctccgat accctccatg ccgttcggcg ccagctcgac ggccctgtgc gccccgactg
     3901 cggcggcgcc gacgaccgcc actccccggg cgagggcgaa gaggatctcc ttgtgccacg
     3961 tcggcaggct ccggtagggg agttc
//
LOCUS       DAOSWW010000125         3969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15787_length_3969_cov_3.009964, whole genome shotgun sequence.
ACCESSION   DAOSWW010000125 DAOSWW010000000
VERSION     DAOSWW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15787_length_3969_cov_3.009964"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PK442_03705"
     CDS             complement(<1..994)
                     /locus_tag="PK442_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86784.1"
                     /translation="MICSHDVKQEITRQEEHVFLDGLSQKEKDGEVQSALLPRGSAEK
                     FFFSGLFFLGLWLLAQGLSAQPVLEILGRLPETMDSGDLLSAASMLVLMNSLRAIALY
                     LGWFLLGSGLGALHPSVSFLSWFVPVVAIPVTYFILPSLGEGVQLHFGFPAVLSVSSV
                     LVLRYLTRGIADWGYKAIALALFVFSFQWLDVIPALTSWGAGWGELSSAVKTAALLME
                     REYLLNRAGIAVFGGLFCAGVITTELLVSYSARLYSLALLRDRENKVALLREENLRNR
                     SLVEMQQLVHDLKRPLTTIMGLADVIASGKSAPSAGKYGAVIVEAGRSMEEMISE"
     gene            complement(991..2055)
                     /gene="pgsW"
                     /locus_tag="PK442_03710"
     CDS             complement(991..2055)
                     /gene="pgsW"
                     /locus_tag="PK442_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOO86785.1"
                     /translation="MNGLSARSRAFARRARWGLLIPACVLAFLWFFQGVSFLSEEEEA
                     LWRKVRAAQTSLAAWRDASQLPALYPEYDFSKTDPWRSGFIGIEWSPVTTTLGPIEAK
                     RTAADPLWSVYALREFRKFGLKEGETIAMISSSSFPGLVYSLLAAAEHLGLRVLWIHS
                     LGASTWGANIPEMSWPKIGELLRNGGFLSRKPDWYTLGGGNESGGGMSPEGRKALLAA
                     ALHDGVPMLEAATLQEMIELKTKHILSVKPRLVLAVGGSHSTFGQADIVPPGGGWYTP
                     EDTERVDMGEGVFRAVLLSGVPVLHFLNLRPLARSAGIPFDVPPRSRFGGGAGDAACA
                     AGLLFYVVFLFRFHRWRRDE"
     gene            complement(2052..2489)
                     /locus_tag="PK442_03715"
     CDS             complement(2052..2489)
                     /locus_tag="PK442_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HOO86786.1"
                     /translation="MTEHSLLLVGLGICLGLFFFHRTGYSPGGIITPGFLALELGSPE
                     RVAAAFVIGGCVAALLSLVVRVTGAYGRQRTGIALLLALAFRLFAGGGTTLSYLWIGW
                     VVPGLIGADMQRQGAIPTIGAALSTAFASAMAARLLISAGALL"
     gene            complement(2486..3541)
                     /locus_tag="PK442_03720"
     CDS             complement(2486..3541)
                     /locus_tag="PK442_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOO86787.1"
                     /translation="MNSIHSIPIRILVTGSRGKSSLVRLLSAALVSFGLNVRGRITGV
                     LPRELLPGDGILSPLKETLLLRSGPASVEEMRWWLSTLPRGTDAVVMENSAVAPELQA
                     LAFRWLQPCCTVLTNVRPDHEEVWGRGEENAASALCSGIGDPGGMTCPEHPVVLPRSV
                     AEKQCVQRLLSAQHCRLLPCGEGNAFKETHLALVQEVCRLFGFDERQSRAAAAALPPD
                     VADFAFFREGDGVLAAAFSANDTESTEQLFVSTGWSREETTLLFNARKDRVRRFRSFL
                     PWIRSFPWKKVCVTGGRPLFLSSPAVYSPLLDAPSLAGFIRSTGRVFGCGNVAGTPLE
                     YLESKFLQFRERQDGVS"
     gene            complement(3635..>3969)
                     /gene="deoC"
                     /locus_tag="PK442_03725"
     CDS             complement(3635..>3969)
                     /gene="deoC"
                     /locus_tag="PK442_03725"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOO86788.1"
                     /translation="SVREDIRAVVQAAEGALVKVIIETCLLTDEQKKLACCLAEEAGA
                     HFVKTSTGFSTGGATVEDVALMRRVVGDRLGVKASGGIRTREDAERMIAAGASRIGAS
                     ASVGICAR"
BASE COUNT          950 a         1214 c         1130 g          675 t
ORIGIN      
        1 tctcggagat catctcctcc atcgaacgtc ccgcctcgac aatgacggcg ccgtatttcc
       61 ccgcgctcgg ggcgcttttt cccgaggcga taacgtcggc cagccccatg atcgtcgtca
      121 gggggcgctt cagatcgtga acaagctgct gcatttcaac aaggctccgg ttccggaggt
      181 tctcctcccg caaaagagcc actttgttct cccggtcccg cagaagagcc aagctgtaga
      241 gacgggcgct gtagctgacg aggagttccg ttgtgatgac tccggcgcag aaaagacctc
      301 cgaagactgc aattcccgct ctgttcagca gatactcccg ttccatcaga agcgctgcgg
      361 tcttcacggc ggaggagagc tcgccccaac ccgcccccca ggatgtgagc gccggaatga
      421 cgtcgagcca ctgaaaagag aaaacgaaaa gggcgagcgc gatggctttg tacccccagt
      481 cggcgattcc tctggtcaga tagcggagga caagcacgct gctcacgctc agcacggcag
      541 ggaagccaaa atggagctga accccctccc ccaacgacgg aagaataaaa tatgtcaccg
      601 gaatggcgac aacaggcacg aaccacgaga gaaaagagac cgaggggtga agcgctccca
      661 ggccgcttcc cagaaggaac cagccgagat agagcgcgat ggccctgagc gagttcatca
      721 ggacgagcat ggacgcggcc gagagaagat cgccgctgtc catcgtctcg ggaagcctgc
      781 cgagaatctc aagcacaggt tgggcggaaa gcccctgcgc cagcagccac aaccccagaa
      841 aaaaaagacc ggaaaagaaa aatttttcgg cgcttccgcg aggaagaaga gcgctttgga
      901 cttctccatc tttctctttc tgagagagac cgtccaagaa cacatgctct tcctgccggg
      961 tgatttcctg cttcacgtca tgggaacaga tcattcgtct ctcctccaac ggtgaaagcg
     1021 gaaaagaaaa acaacgtaga acaggagccc tgcggcgcac gcagcatctc cggccccgcc
     1081 gccgaaacga gagcggggag ggacgtcgaa aggaatccct gcgcttcgtg caagcggccg
     1141 gaggtttaaa aagtgaagca ccggtacgcc cgaaagaaga acagcacgga acaccccttc
     1201 gcccatgtcg actctttccg tgtcttcagg cgtgtaccac cctccgccgg gagggacaat
     1261 atcagcttgc ccaaaggtac tgtgcgaccc tccgactgca agtacaagcc gcggtttcac
     1321 ggaaagaata tgcttggttt tcagctcgat catctcctga agcgttgcgg cttcgagcat
     1381 cggaacaccg tcgtgcagcg ctgcggcaag aagcgccttt cttccttccg gcgacatacc
     1441 gccgccggat tcgttccctc cgcccaaagt ataccaatca ggctttcgtg agaggaagcc
     1501 tccatttcta aggagttctc ctatcttcgg ccaggacatt tccggaatgt tcgcgcccca
     1561 agtggaggcg ccaagcgagt ggatccacag gacgcgaaga ccaagatgct cggcagcggc
     1621 aagaagcgaa tacaccaggc cggggaaaga ggacgaagag atcatggcga tagtctctcc
     1681 ttctttgagc ccaaacttcc ggaattcccg caaagcgtag acgctccaga gaggatccgc
     1741 agctgtccgc ttcgcttcta tcggtccaag agtcgtcgtc accgggctcc attcgatacc
     1801 gatgaaaccg cttctccacg gatctgtttt gctgaagtca tattccgggt acagggcagg
     1861 gagctgcgac gcatcgcgcc acgcagcaag gctcgtctgg gcggcgcgca ccttccgcca
     1921 caaagcctct tcctcctccg aaagaaacga aacgccttga aaaaaccaga gaaaggcgag
     1981 cacgcacgcc ggtatgagta atccccatcg tgcccgacga gcaaaggcgc gggaccgcgc
     2041 gctcaatccg ttcataagag cgctccggcg gaaatcagca aacgggcagc catcgcggag
     2101 gcgaacgccg tggaaagcgc agcgccgatg gtcggaatcg ctccctgccg ttgcatatcg
     2161 gcgccaatca gcccgggaac gacccatcct atccacagat acgagagcgt cgtccccccc
     2221 ccggcgaaca atctgaaggc aagcgcaagc agcagggcga tccccgttcg ctgacggcca
     2281 tacgctccgg ttacacgtac gacgagcgag aggagggccg caacacatcc tccgatgaca
     2341 aatgctgcag cgactctttc cggcgagccg agttcaaggg cgagaaaacc gggcgtaatg
     2401 atgccccccg gggagtaccc tgtgcggtgg aaaaagaaca agcccaggca gatccccagt
     2461 ccgaccagaa gcagactgtg ttccgtcacg atacgccatc ctgcctttca cgaaattgca
     2521 gaaatttcga ctccagatac tcaaggggag tccccgccac gttgccgcac ccgaagaccc
     2581 tgcccgttga acggataaaa cccgcaaggg acggggcgtc aaggagcggc gaatacacgg
     2641 caggggatga taaaaagaga ggtcggccgc cggtaacgca gactttcttc cacgggaagg
     2701 aacgaatcca tggaagaaat gacctgaaac gcctcaccct atcctttctt gcattgaaaa
     2761 gaagagtggt ctcctctctg ctccatccgg tggatacaaa aagctgctca gttgattcgg
     2821 tgtcgtttgc agaaaaagct gccgcaagca ccccgtctcc ctcgcggaaa aaggcgaaat
     2881 cagccacatc cggaggcagc gcggcggcgg cggcacggct ttgccgctcg tcgaagccga
     2941 agagacggca gacctcttgg acaagggcga ggtgcgtttc tttaaaagcg tttccttcgc
     3001 cgcagggaag aagacggcaa tgttgtgctg agagcaggcg ctgcacacac tgtttttcgg
     3061 cgacagaacg gggaagaacg acaggatgct ccggacaggt catacccccg ggatcgccta
     3121 ttcctgaaca gagcgcagac gcggcgttct cttcgcctct tccccaaacc tcctcgtggt
     3181 cgggacgaac atttgtgagc acggtgcagc acggttgaag ccaccggaag gcaagggcct
     3241 gcaattccgg ggcgaccgcg ctgttctcca tcacgacggc gtccgttccc ctgggaaggg
     3301 ttgaaagcca ccagcgcatc tcctcgacgc tcgccgggcc ggaacgaagc agaagagtct
     3361 ccttcagagg agataagatc ccgtcgccgg gcagaagttc tcttgggaga acccctgtga
     3421 tccgtccgcg gacgttgaga ccgaaggaga ccaaagcagc cgaaagaagg cggacgaggg
     3481 agctttttcc ccggctcccg gtaacgagta ttcggatggg aatcgaatga atgctgttca
     3541 tgaagtatat tgtacacctc gcgccgaaag aagaaaaaga aaacggggcc cccgaaagcg
     3601 gcggaaccgc tccgggaacc ccgtgcggaa ttcgttatcg ggcgcagata ccgacggaag
     3661 cggaggcccc gatacgcgaa gctccggcgg cgatcatacg ttcggcgtct tctctcgtgc
     3721 ggataccgcc cgacgccttt acgccaagcc tgtcgcccac gacgcgccgc atcagcgcga
     3781 catcctcgac cgtcgcgcct cccgttgaaa aacccgtcga ggtcttcacg aagtgcgctc
     3841 ccgcctcctc ggcaagacaa caggccagct tcttttgctc gtccgttaac agacaggttt
     3901 ctatgatcac cttcacaagt gcgccttcgg cggcctgtac cacggcgcgg atgtcctcac
     3961 gtacggagg
//
LOCUS       DAOSWW010000126         3963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15826_length_3963_cov_4.313460, whole genome shotgun sequence.
ACCESSION   DAOSWW010000126 DAOSWW010000000
VERSION     DAOSWW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15826_length_3963_cov_4.313460"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PK442_03730"
     CDS             <1..924
                     /locus_tag="PK442_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOO86789.1"
                     /translation="DGAGKAVFLDDVPAEKRGVTLSSAAAIMSFFFNKEVREEEYVLL
                     EDVELFQSISTGLDFLPFRLMPQKMILACNAGGWAPQIAEHTVALLLALTRRIVPLHN
                     DLVKGVFGRHSYSPASLRGKVLTVVGYGGIGRRTADLLRAFGIKIYALNTSGRTNDDV
                     HFMGTLKELPEILPETDVLLLSIPLDKHTKGLIGKRELDLMKKNAILLNVARAPLVVE
                     EDLYNHLKANPEFQAGFDVWWKEPTWGGGDFVLDHPFLDLPNVVGSPHNSNRCENDME
                     NAVMAAAANVAAFFSGEKISGVIRREDYIED"
     gene            938..1723
                     /gene="xth"
                     /locus_tag="PK442_03735"
     CDS             938..1723
                     /gene="xth"
                     /locus_tag="PK442_03735"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOO86790.1"
                     /translation="MARISIATFNVNSVRMRLPILEKWLKDRSIDVLCLQETKVVDED
                     FPVAAFKEMGYRAVFRGEKSYNGVAILTKVPMDGFAIGFGDGEEPECDTRLVRGRIGD
                     FCFINTYVPQGKAVDHADYVFKHRFLDRMKALLKREYLTSEKVVWVGDMNVAPTDIDV
                     TSPEKKRDHPCFAPDIQKHFEDVKSWGFVDLFRKYRPGEGEFSFFDYRVKNSLDRNIG
                     WRIDHVLVTPSLESAAADCFIDRDPRGWEQPSDHTPVVAVFDL"
     gene            complement(1796..3154)
                     /locus_tag="PK442_03740"
     CDS             complement(1796..3154)
                     /locus_tag="PK442_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO86791.1"
                     /translation="MFSWKRIRYGVMFAFLAYISWVGYQHQVLGGGPAGIPTVDALCP
                     LGGLESIYSYLTSGVWLRRVAPSALILFIAVAGITLLLGRVFCGWICPLGTIGEFSNL
                     AAKKLGIRQRELPPALDRGLKMLKYVVLAVILYFTWTWGTLAWREYDPWVSWMHLASG
                     WEEMVASPWGYVVLFFVVIGAGLFIERFWCRYLCPLGAALGILQKFSLTKVRRSKETC
                     ISCSKCNRSCPMGITPMALEKVTDADCIACGRCTESCPVEKTLQFSVAGKKVLSALAV
                     GLLGVGLYFAAYGTAKITGYWQTFASTSVEALKDPVDGIFGWMTIEQVAAQVHLPSAK
                     ILEIAGLPAESPTNVSIKQMEGVNDEELKEQLKAYFAANPAKAPEPKTVVPSNPDELK
                     GSFTLAEVASGYGLELSAILEKAGWPADADPNVKMRDVANTIGRDSSDIRNAVKELLE
                     NR"
     gene            3501..>3963
                     /locus_tag="PK442_03745"
     CDS             3501..>3963
                     /locus_tag="PK442_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86792.1"
                     /translation="MSVSPMAGKRPDHTMLANIPALVASYYTVRPDTDDARSLVAFGT
                     SGHRGSSLKGSFNEAHIIAVSAAIVEYRKSAGITGPLFVGKDTHALSEPAFRTAVEVF
                     AAAGVRTVIQKGHGYTPTPVISRAILQWNKGRSEGLADGVVITPSHNPPEDG"
BASE COUNT          811 a         1171 c         1197 g          784 t
ORIGIN      
        1 gacggcgccg gaaaggccgt cttcctcgac gatgttcctg cggagaagag gggagtgacg
       61 ctctcttccg ccgcagcgat catgtccttc ttctttaata aagaagttcg cgaggaggag
      121 tatgttcttc ttgaagatgt cgaactgttc cagtccatct cgaccggact cgacttcctc
      181 cccttccgcc tgatgccgca gaagatgatt ctcgcatgca acgcgggggg gtgggcgcct
      241 cagatcgcgg agcacaccgt ggcgcttctg ctggcgctga cccggcgtat cgtgccgctc
      301 cataacgact tggtgaaggg agttttcggc cggcactcct actcgccggc gagcttgcgc
      361 ggaaaggtgc tcacggtcgt ggggtacggc ggcatcgggc ggcggaccgc cgatctgctg
      421 cgcgccttcg gcatcaagat ctacgcgctg aacacgagcg gccggacaaa cgacgacgtg
      481 catttcatgg ggacgctcaa ggagcttccg gagattctcc cggagacgga cgtcctcctg
      541 ctctcgatcc ctctcgacaa gcacaccaaa gggttgatcg gcaagcgcga gctcgacctg
      601 atgaaaaaga acgcgattct tttgaatgtg gccagagccc cgctcgtcgt ggaggaggac
      661 ctgtacaatc acctgaaggc gaatccggag ttccaggccg ggttcgacgt ctggtggaag
      721 gagccgacat ggggcggcgg ggattttgtc ctcgatcatc cgttcctgga tctgccgaat
      781 gtcgtcggtt cgccgcacaa ctcgaatcgc tgcgagaacg acatggagaa cgcggtcatg
      841 gctgccgccg ccaatgtcgc ggcctttttc agcggagaga agatcagcgg cgtcattcgc
      901 cgggaagatt acatcgaaga ctaaggagag tgccgtcatg gcaaggattt cgatagctac
      961 attcaacgtc aactcagtcc gtatgcgtct tccgatcctt gagaaatggt tgaaagaccg
     1021 ttcgatagac gttctctgcc tccaggagac gaaggttgtg gacgaggatt ttcccgtcgc
     1081 cgcgttcaag gagatggggt atcgggccgt ctttcgcgga gagaagtcct ataacggggt
     1141 cgccatcctg acgaaagtcc ccatggacgg atttgccatc ggcttcggcg acggcgagga
     1201 gcccgagtgc gacacccgcc ttgtccgcgg gcgcatcggc gacttctgct tcatcaacac
     1261 ctacgttccc cagggaaagg cggtcgacca tgcggactac gtcttcaagc accggttcct
     1321 cgacaggatg aaggcgttgc tgaaacggga atacctgact tcggagaagg tcgtctgggt
     1381 gggggacatg aacgtggccc ccaccgacat cgacgtcacc agcccggaga agaagcgcga
     1441 tcacccgtgc ttcgccccgg acatccagaa acacttcgag gacgtgaaga gctgggggtt
     1501 cgtcgatctc ttccggaagt accgcccagg cgaaggcgag ttttcctttt tcgactatcg
     1561 ggtgaagaat tccctcgacc ggaacatcgg ctggagaatc gaccatgtgc tggtgacgcc
     1621 ctctctggag agcgctgcgg cggactgttt catcgaccgc gatcccagag ggtgggagca
     1681 gccctccgac catacgccgg ttgttgctgt attcgacctg taagctcata ggtacgaaaa
     1741 agcgggggag agattccccc gctttcttat tccgtttccg tcctcccgtg ttcgatcagc
     1801 gattctccag cagctccttc accgcgttcc ggatgtccga ggagtcgcgc ccgatcgtat
     1861 tcgccacgtc gcgcatcttg acgttcggat cggcgtccgc aggccaccct gctttttcga
     1921 ggatggcgga gagttccaga ccgtaccccg acgccacctc cgcgagcgtg aagcttccct
     1981 tcagttcgtc cgggttggac ggcacgactg tcttcggttc aggagccttg gccgggtttg
     2041 ccgcgaagta ggccttgagc tgctccttga gctcctcgtc gttcacgcct tccatctgct
     2101 tgatcgagac gtttgtgggc gactcggcgg gaagcccggc tatctccaga atttttgccg
     2161 acggaaggtg tacctgcgca gccacctgtt cgatggtcat ccacccgaag atgccgtcca
     2221 cgggatcctt cagcgcctcg accgaggtgg aggcgaacgt ctgccagtag ccggtgatct
     2281 tcgccgtgcc gtaggccgcg aagtagaggc ccacgccgag cagtccgacc gcgagcgccg
     2341 ataacacttt cttcccggcg acggagaact gaagcgtttt ctcgaccggg cagctctccg
     2401 tgcagcggcc gcaggcgatg cagtccgcgt cggtgacttt ttcaagcgcc ataggcgtta
     2461 ttcccatcgg gcacgagcgg ttgcacttgg agcaggagat gcaggtctct ttgctgcgcc
     2521 gcaccttcgt caaactgaac ttctgcagga tgccgagggc tgcgcccaga gggcagagat
     2581 agcggcacca gaaacgttcg atgaagagcc ccgcgccgat gacgacaaag aagagaacga
     2641 cgtaccccca cggagaggca accatctcct cccatccgga ggcgaggtgc atccacgaga
     2701 cccatgggtc gtattcgcgc catgcaagcg ttccccaggt ccatgtaaag tagagaataa
     2761 cggcgaggac gacgtacttc agcatcttca aaccacggtc aagcgcgggc ggaagctccc
     2821 gctgccgtat cccgagcttt ttggccgcga gattactgaa ttctccgatg gtccccaggg
     2881 ggcagatcca cccgcagaag accctgccga gcagcagcgt gatgcccgcg acggcgatga
     2941 agaggatcag cgcgctgggg gcgacgcggc ggagccagac gcccgaagta aggtagctgt
     3001 agatgctttc aaggcctccc agaggacaga gggcgtcgac ggtcggtatt cccgccggtc
     3061 ctcctcccaa tacctgatgc tgatacccca cccaactgat gtacgccaga aaagcgaaca
     3121 tgacgccgta ccggatacgc ttccatgaaa acatgcagat cctccccctc gatgagaatt
     3181 ttctaataga atagcaccat ccggactgga atactattgt ttctttatac actccggggg
     3241 tacgtcatat tacggagagc agagaggagc atccgtaggc tcatgcacag aatgcgcgca
     3301 gtgcaatccc ctcttcgcct gcggattcgg gcaggtacgg aagtgaatga aagagaattt
     3361 tctagtataa tatcctttga atctcctggt attgttgctt cgttatcccg tgcggcagct
     3421 gttgcgcgcg gaagacttcc ggccgtgtcg aaaacccgtc ccgggccgac atgtatgttg
     3481 atgaacaggg gggaatcacg atgagcgtat cgccgatggc cggcaaacgc ccggaccata
     3541 cgatgttggc aaacataccc gcgctggtcg catcctacta cacagtccgt ccagacacgg
     3601 acgatgcgcg ctcgctcgta gccttcggaa cgtcggggca caggggatct tccctgaaag
     3661 gttccttcaa cgaggcgcac attattgcgg tcagcgcggc gatcgtcgag taccggaaga
     3721 gcgcggggat tacagggccg ctcttcgtcg gcaaggatac gcacgccctc tccgagccgg
     3781 cgttcaggac ggctgtggaa gtttttgccg ccgccggcgt ccggacggtg atccagaaag
     3841 gacacggata cacgccgacg ccggtaattt cgcgcgccat tctccaatgg aacaagggga
     3901 gaagcgaggg acttgccgac ggcgtcgtca tcacaccatc gcacaacccg ccggaggacg
     3961 ggg
//
LOCUS       DAOSWW010000127         3953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_15895_length_3953_cov_4.160595, whole genome shotgun sequence.
ACCESSION   DAOSWW010000127 DAOSWW010000000
VERSION     DAOSWW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15895_length_3953_cov_4.160595"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK442_03750"
     CDS             complement(<1..307)
                     /locus_tag="PK442_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86793.1"
                     /translation="MYCRFKCVPAILFLFLLLLVLPPSGAESATPAPKNEVTRAEFFL
                     KKFEDRVNRFRGQSFRLGFEDNEALKRIRDLKEKYPEDPAVEDLYQRARMALMKSKGD
                     "
     gene            complement(497..1930)
                     /locus_tag="PK442_03755"
     CDS             complement(497..1930)
                     /locus_tag="PK442_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO86794.1"
                     /translation="MRIYKTLAAAAGMGLLFAWMESVFLQYGLLQRLAAIEYEGMNAV
                     VIFLAVHALTYLFAAFFAPGEKGERPRCAIAACLMLSLPLLIGLAQYLPASFSAVAAL
                     SGMIFSSVGAALLIGLWALFLSSLIPDDVALVFGLAPLVAAFVMRFAALSHILLLILG
                     APLFSALLLLLPSSDAHDESPDAIQSSSFPFWRLALFLFCLYAANGFLLSLMPELFPS
                     PFPRTDQSLDWIPTISALGVAVVFRFYPRAELRTFYKGAFPFIAASLFLLLFSPLKTL
                     SRFFMETGLAFLDLYAWLLLIYFASRAGEKRNAVIHGGIFLIVLAGATAHLHLLLRNG
                     AGGAIHGASELLIVVIPGVCLLLIMLALWDGREIPLQWERQDTRSFPERDSGEETNFD
                     HEKMPHPHEESASEKEEEMLLRMKLMEFNLTRQETEIALLLLQGLKDRNICSLLYISQ
                     NTLKYHLRNIYRKTGSANRLELKNNVD"
     gene            2085..2639
                     /locus_tag="PK442_03760"
     CDS             2085..2639
                     /locus_tag="PK442_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOO86795.1"
                     /translation="MSVKNAMTAEFLRSAYGGESMAHMRYLGWADKADKEGFPKIGRL
                     FRAVAYAEQVHATNHFVVLNGDVADTALTAGAVFGMKETAENLVGGIMGEEHEVEQMY
                     PVYYEAAKFQGEKDAMRTFNFALQAEKQHAELFRAARECALAKKDMEFEAVYVCPVCG
                     HTVTDEAPEKCPVCGVAKEKYARF"
     gene            2726..3409
                     /gene="sdaAB"
                     /locus_tag="PK442_03765"
     CDS             2726..3409
                     /gene="sdaAB"
                     /locus_tag="PK442_03765"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HOO86796.1"
                     /translation="MSFLDIVGPVMIGPSSSHTAGAARLGRLAKACWGTDTLNEVTVF
                     LRGSFAFTSKGHGTDKAVVAGLMGMLPDDERIRNALMLAKEAHFPYRLTTEDVDGAHP
                     NSARFSFLGKNGRRLDVVGASIGGGAVEIQEIDGFSVAINGELPTLITFHKDTRGVVA
                     AVAALMAGREINIASMTLHRKTRGGVATLVTELDMNAPPDEQLCNDVLQAHPAILRVA
                     SLFGGGNNS"
     gene            3406..>3953
                     /locus_tag="PK442_03770"
     CDS             3406..>3953
                     /locus_tag="PK442_03770"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOO86797.1"
                     /translation="MRSLAEIFAVCASSGSSFTHAAIEIETSESGCSVEMLRKGMLSR
                     LKDMEFSVNEALSGSWRGLLVPEEAYLLHEYGADAASFSSPFVLTAARIAIAVSTYNA
                     AMGRIVAAPTAGSCGILPGILFSYRDHFRCDDETLLSGLFAAAAVGSVTAARATLAGA
                     SGGCQAECGAATAMGAAALVQMR"
BASE COUNT          945 a         1089 c         1129 g          790 t
ORIGIN      
        1 aatcgccctt gctcttcata agcgccatgc gcgctctctg atagagatct tcgacagcag
       61 gatcctccgg gtacttctcc ttaagatccc gaatacgttt cagcgcttcg ttgtcctcga
      121 atccgaggcg gaacgactgt cctctgaaac ggttcaccct gtcttcgaat tttttaagga
      181 agaactcggc gcgagtcacc tcgttttttg gagcgggcgt cgcggactcc gctccggagg
      241 gcggcaatac aagtaggagg aggaaaagga aaagaatcgc cggcacacac ttgaatcgac
      301 aatacattgt gcatcatctc ctctcgtttc cacggttcgt ctatacaagt ggattgtagc
      361 atcaaagaga gaaagtcacc cccccgccgc gaactaaaag gggtgggatt tcccacccct
      421 tttaggggtg atattcccca cctttttttc tatcgcatgg ggtgaccggc atcacccttg
      481 atgattccct ttttcgttag tcaacgttat ttttcagttc gagacgatta gcggaccctg
      541 tcttgcgata gatattgcgg aggtggtact tgagggtatt ctgcgagatg taaagtaatg
      601 aacagatatt cctgtctttg agcccctgaa gaagaagaag ggcgatttcg gtttcctgcc
      661 gtgtaagatt gaattccatc agcttcatgc gaagaagcat ctcctcctcc ttctccgatg
      721 cgctttcttc atgaggatga ggcatttttt catgatcgaa atttgtttcc tctccactat
      781 cccgttccgg aaaggagcgt gtatcctgac gctcccactg aagagggatt tcccttccat
      841 cccagagcgc aagcatgatg agaagaaggc aaacaccggg gatgacaacg atcaggagct
      901 ccgacgctcc atggattgca ccaccggcgc cgttacggag cagcaaatga agatgcgccg
      961 tagcgccggc cagtactata aggaaaatgc ctccgtggat aaccgcgttc cgtttctccc
     1021 ctgccctgga ggcaaaatag ataagaagca gccaagcgta caggtcaaga aacgcgagtc
     1081 ccgtctccat aaagaagcgg gacagcgtct tcaagggaga gaagagcaaa aggaagagcg
     1141 acgctgcgat gaacgggaac gcgcctttgt agaacgtacg cagttcggct ctcgggtaaa
     1201 agcgaaaaac cacggcaacc cccaaggcag atatcgtcgg tatccagtct aaagactggt
     1261 ctgtccgggg aaaaggcgag ggaaagagct ccggcatcaa actgagaagg aaaccgttgg
     1321 cggcataaag acagaaaagg aagagcgcaa gccgccagaa agggaaagaa gaggactgta
     1381 tggcgtcagg agattcatcg tgggcatcgc tgctcgggag tagcaggagc aatgccgaga
     1441 agagcggcgc tcctagaatc agcagcaaga tatgagaaag cgctgcaaaa cgcattacga
     1501 atgcagcgac aagcggcgcc aaaccgaaga cgagtgcaac atcgtcagga atgagtgagc
     1561 tgaggaaaag agcccacaat ccgataagaa gggctgctcc gacggatgag aaaatcattc
     1621 cggaaagcgc tgcaacggcg gaaaacgaag caggaagata ttgagcaagg ccgatcagga
     1681 gaggaagcga gagcataagg catgcagcaa tcgcacaccg gggacgctcc cccttctcgc
     1741 cgggcgcgaa aaaagcggca aaaagatagg tcagggcatg cacggcaaga aaaatgacga
     1801 ccgcgttcat tccttcgtac tctattgctg caaggcgctg caataatccg tactgaagaa
     1861 agacggattc catccaggca aaaagcaacc ccatgcccgc cgctgcggcg agagtcttat
     1921 atatcctcac cggaagcctc cttccggaac agagtattgt gctgcattga tgataccata
     1981 ttccgtcttt ttacagggta tcttctcacc cctttccaca tgcttaaaaa atgtgatatc
     2041 ctttctttca agatacctat tatttcaaag ggggatgtgt tttcatgtcc gtaaaaaacg
     2101 ccatgaccgc agagtttctc cgttccgcct acggcggaga gagtatggcc cacatgcgct
     2161 acctcggctg ggctgacaag gcggataaag agggctttcc aaagatcgga cgacttttca
     2221 gggcagtcgc ctacgccgag caggtgcacg cgacgaatca cttcgtcgta ctgaacggcg
     2281 atgtcgccga taccgcgctc actgccggag ccgtcttcgg aatgaaggag accgcggaga
     2341 atctcgtcgg cggcatcatg ggcgaggagc acgaagtcga gcagatgtac cctgtgtact
     2401 acgaggccgc aaagttccag ggcgaaaagg acgcgatgcg gacattcaac ttcgcgctcc
     2461 aggccgagaa gcagcacgcg gaacttttcc gcgccgcccg cgagtgcgcc cttgccaaga
     2521 aggacatgga gttcgaggcc gtgtacgtct gtcccgtgtg cgggcatacc gtcaccgacg
     2581 aagcgccgga aaagtgcccg gtgtgcggcg tagcgaagga aaaatacgcc cgattctaga
     2641 agttcttcca aggtagcctg ttccaggggg aggagcgcag cttcctcccc ctgcttgcta
     2701 taacgcacgg aaagggaggc gagccatgag ctttctcgat atcgtcggcc cggtgatgat
     2761 aggcccctcg tcgagtcata cggcgggagc ggcccgcctg ggcagacttg caaaggcatg
     2821 ctgggggacc gacactctta acgaagtaac cgtctttctc cgcggcagtt tcgccttcac
     2881 cagcaaagga cacggcacgg acaaggccgt cgttgccggt ctgatgggaa tgctgcccga
     2941 tgacgaacgc atccggaacg cgctcatgct ggcgaaagaa gcacactttc cttatcgcct
     3001 gacgaccgag gacgtggacg gggcgcaccc caactccgcc cgtttctcct tcttggggaa
     3061 aaacgggcga agactcgacg tcgtcggcgc ttcgatcggc ggaggcgctg tcgaaattca
     3121 ggagatcgat ggtttctcgg tcgccatcaa cggcgagctg cctacgctca tcacatttca
     3181 taaggatacg cgcggcgttg tggcggcagt tgccgcgctc atggcgggac gggagatcaa
     3241 catcgcctcc atgacgctcc atcgaaagac gcggggcgga gtggcaaccc tcgtcacaga
     3301 gctcgatatg aatgcacctc ctgacgagca gctgtgtaac gacgtactac aagcgcaccc
     3361 ggcgattctc cgcgtcgcat cgctgtttgg cggaggaaac aattcatgag atccctggcc
     3421 gagatcttcg cagtatgcgc atcgtcaggg agctccttta cacacgccgc catcgaaatc
     3481 gaaacttcgg aatccggctg ttccgttgag atgctgcgga agggaatgct ctcccggctg
     3541 aaggatatgg agttctctgt caacgaggcg ctctccggct cctggagagg gcttctcgtt
     3601 cccgaggagg catatcttct gcacgaatac ggcgcggacg cggcatcttt ttcaagcccg
     3661 ttcgtcctca ccgccgcccg tatcgccatc gccgtctcca cgtacaacgc ggcgatggga
     3721 cgcatcgtcg ccgctccgac cgccggaagc tgcggtattc tgccggggat actcttctcg
     3781 taccgggatc atttccgctg cgacgacgaa acgctcctct ccgggctttt cgccgcagct
     3841 gcggtcggtt cagtgactgc tgcgcgcgca acgctcgcgg gagcctccgg aggctgtcag
     3901 gccgagtgcg gcgcggctac ggcgatgggc gctgccgcct tggtacagat gcg
//
LOCUS       DAOSWW010000128         3910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16154_length_3910_cov_2.894423, whole genome shotgun sequence.
ACCESSION   DAOSWW010000128 DAOSWW010000000
VERSION     DAOSWW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16154_length_3910_cov_2.894423"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PK442_03775"
     CDS             <1..894
                     /locus_tag="PK442_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO86798.1"
                     /translation="YMPAEPEEKELALHDGVELMELLAPVKYDGKTLLCEKMQLGEKD
                     ASGRRGVTGTGEMVPVAADTVIGATGAKVDSEIFGANGIALDAKGRVVMNEENETSVS
                     NVYVAGDCKAGPATVVKGIADAKTVTKSILAKEGLSPDFAVTELVQDLASLYEKKGVL
                     KEAEKDQNDGARCLACDVVCELCCDVCPNRANVLVVVDGKHQIVHLDGMCNECGNCGI
                     FCPHTGDPYKDKVTLFWTEEDFKESTNKGFFRVEGDRYSVRVEDGSVVSWRRGEPGVS
                     LEMAKTLGAVLERYPYYMMNL"
     gene            907..2235
                     /gene="ssnA"
                     /locus_tag="PK442_03780"
     CDS             907..2235
                     /gene="ssnA"
                     /locus_tag="PK442_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOO86799.1"
                     /translation="MLLIGNGKVITRDENNTVIENGAVVIDGAHIKAVGKTEDLKKAY
                     PGATFKDAKGRLVMPGLINVHMHYYSTFARGIALDSPPATKFSEILEGLWWRLDKCLT
                     LEDVYYSVMGPGIDQIKCGVTSVIDHHASPFAVRGSLAKIAEASAELGLRSNLCYEVS
                     DRDGEQSRVDGITENIEFIKACAAKKDDMLKGMFGLHASMTISDKTLNESVEQMAGLD
                     AGFHVHVAEGIEDLEDSKAKYGLGVVERWHKHGVLGPKSLAIHCIHISEKEMDLLKES
                     DVAVAHNPESNMGNAVGLSPVLPMMKKGVLVGLGTDGYTADMFESYKVANCLHKHGAK
                     LPSVAWTEPPTMLFENNKKIMNRQIDGEVGTLKPGAYADVVIVDYDPPTPLHGGNING
                     HLLFGVCGRHVSTTIANGKVLMDERKLVHVDEEKIMAESRALAKKLWERI"
     gene            2384..>3910
                     /locus_tag="PK442_03785"
     CDS             2384..>3910
                     /locus_tag="PK442_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOO86800.1"
                     /translation="MTERLNVVGANVPRHDALAKVTGRARYVADLPCDGAWVGGALWS
                     RTPRARVRNIVFSPDFDWSRVTVITARDLPGPNEVHMIKDDYPILAGDEISYIGQAIA
                     LVAAPDKQTIEEALGAMRPDLEELPPVLSVEASLAKEAVIWGEDNVQAHYRIEHGDLE
                     EAFSRSDVIIEETYRTHHQEHLYLETNGMFARPRPDGGVIVEGSLQCPYYVARALAHA
                     LGLPLEKVTMRQTETGGAFGGKEDYPSVLAIQTALLSMKSGKPVRMVLERREDIDVTT
                     KRHPSVIRHRTGVTRDGVILAAEIDIIFDGGAFSTMSPVVLSRGVLHAWSAYRLPNAR
                     IVGRALATNTAPNGAFRGFGAPQTVFAMERQMDRIARELGLSPLEVRRKNLLKTGDTL
                     PCGQVLRSAYAGLVLERALDISGYEEKHGKQQEDDRILRGVGVSVFLHGGGFTGAGEE
                     KISGRAAVEVADDGIVEVLVSSTEMGQCAATVLPQIAAEVLSLAAWKVRHPNPDTSRV
                     P"
BASE COUNT          875 a         1085 c         1235 g          715 t
ORIGIN      
        1 tatatgcctg ccgaaccgga agagaaggaa ctggcgctgc acgacggggt ggagctcatg
       61 gagctgcttg cccctgtgaa gtacgacggg aagacgctcc tctgcgaaaa gatgcagctc
      121 ggcgagaagg acgcctcggg gcggcgcggc gtgaccggta cgggcgaaat ggtcccggtg
      181 gcggcggaca cggtcatcgg cgcgaccggc gcgaaggtag actcggaaat cttcggagcc
      241 aacggcatcg cgctcgacgc caaggggcgc gttgtaatga acgaagaaaa cgaaacctcc
      301 gtttcgaacg tctacgtcgc aggtgactgc aaggcaggac cggcaacggt ggtcaagggg
      361 atagccgacg cgaaaaccgt gacgaagagc attcttgcaa aggaggggct ctcccccgat
      421 ttcgcggtca cggagcttgt gcaggatctg gcgtcgttgt acgagaaaaa aggcgtcttg
      481 aaagaagccg agaaagatca gaacgacggc gcccgctgcc tcgcatgcga tgtcgtctgc
      541 gaactctgct gcgacgtctg ccccaacagg gccaatgtcc tcgtggttgt ggacggaaaa
      601 caccagatcg tgcatcttga cggtatgtgc aacgagtgcg gaaactgcgg cattttctgc
      661 ccgcataccg gagatcccta caaggacaag gtcacgctct tctggaccga ggaggatttc
      721 aaggagagta caaacaaggg attcttccgc gtcgaaggcg accggtactc tgtgcgtgtc
      781 gaagacggtt cggtcgtctc ctggcgccgc ggcgaaccgg gcgtctccct tgaaatggcg
      841 aagacgctcg gagcagtgct cgagcgctac ccctactaca tgatgaatct ctaggaggtg
      901 cacggcatgc ttttgatcgg caacggaaaa gtcatcacaa gagacgagaa caacacagtc
      961 atagagaacg gcgcggtcgt gatcgacggc gcgcatataa aggcagtggg gaagaccgag
     1021 gatctgaaga aggcctaccc cggcgcgacg ttcaaggacg cgaaagggcg gcttgtgatg
     1081 cccggcctta tcaacgtcca catgcactat tacagcacgt tcgcgagagg catcgcgctc
     1141 gacagtcctc ctgcgacgaa attctccgaa attcttgaag ggctctggtg gaggctcgac
     1201 aagtgcctta cgctcgaaga cgtctactac agcgtcatgg ggccgggaat cgaccagatc
     1261 aagtgcggcg tcacgtcggt cattgatcat cacgcgagcc cgttcgcggt tcgcggcagt
     1321 cttgcgaaga tcgccgaagc ttcggcggag ctgggccttc ggagtaatct ctgctacgaa
     1381 gtctccgacc gcgacggaga gcagagcagg gtggacggca ttaccgagaa tatcgaattc
     1441 atcaaggcgt gcgcggcgaa gaaggacgac atgctcaagg gtatgtttgg actgcacgcg
     1501 tccatgacca tctcggacaa gacgctgaat gaaagcgtgg aacagatggc ggggcttgac
     1561 gcgggattcc acgtgcacgt cgccgaaggc attgaagacc tcgaggacag caaggcgaag
     1621 tatgggctgg gcgtggtcga gcgctggcac aagcacggcg ttcttggtcc caagagtctc
     1681 gcaatccact gcatccatat ctccgaaaag gagatggatc tgctgaagga atcggatgtc
     1741 gctgtcgcgc acaacccgga gtcgaatatg gggaacgcgg tcggcctctc tccggtgctg
     1801 ccgatgatga aaaaaggcgt cctcgtgggt cttggtaccg acgggtacac agccgatatg
     1861 ttcgaatcgt acaaagtggc gaactgcttg cacaaacatg gggcgaagct cccgagcgtc
     1921 gcgtggacgg agccgccgac gatgctcttc gagaacaaca aaaagatcat gaaccggcag
     1981 atcgacggcg aggtcggaac gctgaagccc ggcgcatacg ccgacgtcgt catcgtggat
     2041 tacgatcctc cgacgccgct tcacggaggc aacatcaacg gacaccttct cttcggcgtc
     2101 tgcggcaggc atgtcagcac cacaatagcc aacgggaaag tcctgatgga cgagcggaaa
     2161 cttgtccacg tggacgagga aaaaataatg gccgagagcc gcgctctggc caaaaaactc
     2221 tgggagcgga tttaacgaaa agacgatcgt tgccggaggg gtgagggagc gccctttgaa
     2281 gcgactatgc gtcgcgccgg agggcgcttc ctcacgggct gtacctgtcg gcggcgttca
     2341 gtcttctcgt agataatccc atttttaagg aggatgcatg cccatgaccg aaaggctgaa
     2401 cgtggtcggc gcaaatgtgc cgcgacatga cgcccttgcg aaagtaacag gccgcgcccg
     2461 ctacgtcgcc gatctgccct gcgatggcgc atgggttggg ggagcgctgt ggagcagaac
     2521 tccgcgtgcc cgggtgcgaa atatcgtatt ttcccccgat tttgactggt cacgcgtgac
     2581 ggtgattacc gcgcgcgatt tacccggccc caacgaagtg cacatgatca aggacgacta
     2641 tccgatcctc gccggcgacg aaatctccta tattggtcag gcgatagctt tggttgccgc
     2701 gcccgataaa caaaccattg aagaggcgct cggcgcgatg cggccggatc tggaggaact
     2761 ccccccggtg ctcagtgtgg aggcttctct tgcaaaagaa gccgttatct ggggcgagga
     2821 caatgtccag gcgcactacc ggatagagca cggagacctc gaagaggcgt tttcccgttc
     2881 ggatgtgata atcgaagaga cctacagaac gcatcatcag gagcatctgt accttgagac
     2941 aaacggtatg tttgcccgcc ctcgaccgga tggcggggtc atcgtcgaag gttctcttca
     3001 gtgcccgtac tacgttgcca gagcgcttgc gcatgccttg gggcttcctc tggaaaaggt
     3061 gacgatgcgc cagaccgaga ccggcggcgc tttcggcggt aaggaggact atccctcggt
     3121 actggccatt cagactgcgc tgctttccat gaaaagcgga aaacccgtac gaatggtgct
     3181 tgaacgcaga gaggatatcg acgtcaccac aaagcgccac ccgtccgtca tccgccatcg
     3241 cacgggagtc acgcgcgacg gcgttatcct cgcagctgaa atagacatca tctttgacgg
     3301 cggcgcgttc tccaccatga gcccggtggt tctctcacga ggcgtgctcc atgcgtggag
     3361 cgcctacagg cttcccaatg cccgtattgt cggcagggcg ctggcgacga ataccgcccc
     3421 caacggagcg ttccgcggtt tcggcgctcc gcagactgtt tttgccatgg aacgtcagat
     3481 ggacaggata gcccgcgagc ttgggctctc tccgcttgaa gtgcgacgga aaaacctttt
     3541 gaaaacagga gatacgctcc cgtgcggcca ggtgctgcgt tcggcctatg ccggccttgt
     3601 gctcgaaagg gcgctcgaca tatccggata cgaggaaaaa catggaaagc agcaagagga
     3661 tgaccgcatt ctgcgcgggg taggcgtctc cgtcttcctg cacggcggtg gattcaccgg
     3721 ggcgggtgag gagaagatct ccggccgcgc cgctgtggaa gtagccgacg acggaattgt
     3781 cgaggtgctc gtcagcagca ccgagatggg ccagtgcgcc gcgacggtgc ttccgcagat
     3841 agcggcggaa gtcctctctc ttgccgcctg gaaggtgcgg catccgaatc ccgatacttc
     3901 gcgcgtcccc
//
LOCUS       DAOSWW010000129         3901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16210_length_3901_cov_3.214249, whole genome shotgun sequence.
ACCESSION   DAOSWW010000129 DAOSWW010000000
VERSION     DAOSWW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16210_length_3901_cov_3.214249"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PK442_03790"
     CDS             <1..669
                     /locus_tag="PK442_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86801.1"
                     /translation="KMTADTMQYDSASGYFTATENVKIVREGVTATSRKAEGNMNGGE
                     LNLIGDVHIFGVWEDEKVDIKGQILTGYLQDPRGLSFKGTVQGVWGSKQVDAEDLSTR
                     GDTFSVAKLRRFADSAEGFSFSCNSIKGKLKDGEIVEFTAVGAFRFVSSQKKGTPTEI
                     RGHRAVYSKARGSLVVNGNVSAVQDGRSLRSDSLVYFPVKNRIEATGKPQLIFKIDEG
                     SKKQ"
     gene            666..1391
                     /gene="lptB"
                     /locus_tag="PK442_03795"
     CDS             666..1391
                     /gene="lptB"
                     /locus_tag="PK442_03795"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOO86802.1"
                     /translation="MSKMLSARGLSKAYKGRTVVSSADLDVAMGEVVGLLGPNGAGKT
                     TSFYMIVGLIKPDAGVVTVDSRDLTDLPVYRRARLGVGYLPQEASVFRNLSVRENIEL
                     VLQESPPPGENPSIVADRLIEDFGLEKIAHVRGYSLSGGERRRVEIARSLAILPNFLL
                     LDEPFSGIDPIAVYDIQQIILGLRAKGYGILLTDHNVRDTLAITDRTYLIHQGKIVIE
                     GSPDEVAESEVARKFYLGEQFSW"
     gene            complement(1364..1843)
                     /locus_tag="PK442_03800"
     CDS             complement(1364..1843)
                     /locus_tag="PK442_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOO86803.1"
                     /translation="MRHTVLWCVGNPLLQDDGAGPALFTILEGAAPEQIRVVNCKTTP
                     ENYIAPLTRAIRNDSVHLLIVDAAEMGLPGGAVRRMRLDEFAGVAFGTHGIPLPLLLA
                     PLLPELEVTVLGIQPARRGFGDSLSPEVSLAVETLAPLLLEGRWDEVEAYQENCSPR"
     gene            1902..3488
                     /gene="murJ"
                     /locus_tag="PK442_03805"
     CDS             1902..3488
                     /gene="murJ"
                     /locus_tag="PK442_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOO86804.1"
                     /translation="MAKSTHTMVRHALSMMLGTFASRVLGLVREIITAAWFGASGVLD
                     AFNVSFTLANLARQLLAEGALSASFVPVFSRVLAAKGKESAERLARQAFSVLLVATIL
                     SVAAGVVFSPLLVKIMAPGFDPVKAELATAMTRWMFPFLVLVSLAALAMGVLNSMGSF
                     LVPALAPALSNLVYIVLVVFLASFYGVWGLVIAVLAGGACQFLLQWAWSVRMGVTLLP
                     ERPQLKDPDLRTMLALFLPYAAGLSLNQVNPVISRMLASFLQEGAISVLNYANRVIQL
                     PLGIFVIAISQAVLPQLSRCPAEDTGEFRDIMRDSLRFTLFVVFPATLGLALVSDEIV
                     HLLFVRGAFGEWAWKGTSVSLAMYSLGLPGMAFSTVVMRGLYARSLPRGALIVTLSSV
                     AATLLFSLALMGPLSFGGLALATSLAFSLSGWVGVRILSRHLGMRFGIFSVPWTAKMG
                     VSLAFTGLIVVAWRWFFPYSPESSGILRAAWVGGVFCAAASGYAAVTALLRFQEWRWL
                     FGALRRKDRRETPGQNAKEE"
     gene            3492..>3901
                     /locus_tag="PK442_03810"
     CDS             3492..>3901
                     /locus_tag="PK442_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86805.1"
                     /translation="MTFRKLLPCVCVLALLLPVQSFAGDVESLLEKRSAILWFEGEEF
                     GELIIGARAQFAFVYVDGALSEAVVKEPGAPDWLRDNIYYYGSKETRKKALFIVRLRT
                     VKNLDLQLSMIKIGTHTLTPEDVLTNKHYVPVGEL"
BASE COUNT          698 a         1159 c         1214 g          830 t
ORIGIN      
        1 aagatgacgg cggataccat gcagtacgat tccgcaagcg gatacttcac ggcgaccgaa
       61 aatgtgaaga tcgtccggga gggcgtgact gcgacctcca gaaaagccga gggaaacatg
      121 aacggcggag aactcaatct tatcggcgac gttcatatct tcggagtgtg ggaagacgaa
      181 aaagtcgata taaaggggca gatcctgacc ggatacctcc aggatccgcg cggtctttcc
      241 tttaaaggaa ccgtgcaggg cgtctggggg agcaaacagg tggacgccga ggatctgagc
      301 acgcgcgggg atacgttctc cgtagcgaaa cttcgccgtt tcgccgattc cgccgagggg
      361 ttctcctttt cgtgcaactc gatcaagggg aagctcaaag acggggagat cgtcgagttt
      421 accgccgtcg gcgccttcag attcgtctcg tcccaaaaaa aaggaacgcc gacggagatc
      481 cgcggccaca gggcggtcta ctccaaggca aggggatcgc tcgtggtgaa cgggaacgtc
      541 tcggccgtcc aggacgggcg ttcgctccgc tccgattcgc tggtctactt tcctgtaaag
      601 aaccgcatcg aagccacggg gaagccgcag ctgatcttca aaatagacga aggttcaaag
      661 aaacaatgag caaaatgctc tcggctcgcg gcctgtccaa agcctataaa ggcaggacgg
      721 tcgtctcctc ggcagacctg gacgtcgcaa tgggcgaagt ggtgggactg ctcggaccta
      781 acggggccgg aaagacgacg tcgttctata tgattgtcgg gcttatcaaa ccggacgccg
      841 gagtggtcac cgtggattcg cgggatctga cggatcttcc cgtctaccgg cgggctcgtc
      901 tcggcgtggg gtaccttccc caggaggcgt cggtttttcg caatctttcc gtccgtgaaa
      961 acatcgaact cgtcctccag gaaagtccgc cccccggaga aaatccgtcg attgtagccg
     1021 accgcctgat cgaagatttc ggcctggaga agatcgccca tgtccggggc tactccctca
     1081 gcggcggcga acggcgcagg gtggagatcg cccgcagcct cgccattctg ccgaacttcc
     1141 tccttctcga cgagcctttc agcggtatcg accccatcgc cgtctacgat atacagcaga
     1201 tcatcctcgg cctccgcgcg aaggggtacg gtattctcct gacggatcac aacgtccgcg
     1261 acacccttgc catcacggac aggacgtacc ttatacacca ggggaagatc gtcatcgaag
     1321 gctctcccga tgaagtggcg gagagcgagg ttgcgcggaa gttctacctg ggagagcagt
     1381 tctcctggta ggcttccacc tcgtcccacc gtccttccag gaggagggga gcgagcgtct
     1441 cgacggccag ggaaacttcc ggtgaaagcg aatcgccgaa acctctccgg gcgggctgaa
     1501 tccccaggac agtcacttcg agctccggaa gcagcggtgc aaggagcagc ggcagcggta
     1561 ttccgtgcgt tccgaaggcg acgccggcga attcgtccag ccgcatcctc cgtacggctc
     1621 ctccgggaag ccccatctcc gccgcgtcga cgatgagaag atgcacgctg tcgttccgta
     1681 tggcccgcgt cagcggagca atatagtttt cgggcgtcgt tttgcagttc acgacgcgaa
     1741 tctgttcggg ggcagcgccc tccaagatgg tgaaaagtgc gggccccgct ccgtcgtcct
     1801 gaagaagcgg gttcccgacg caccacagta cggtgtgccg cattcgcgca ttcgctcctt
     1861 tcgaaaaaaa cttttaaagt tgaaacaagg aggccggttc cgtggccaag tcaacgcata
     1921 cgatggtacg ccacgctttg tcgatgatgc tggggacgtt cgcgagcagg gtgctcggtc
     1981 ttgtccggga gatcatcacg gcggcgtggt tcggcgcatc gggggtgctc gacgcgttca
     2041 acgtctcctt cacgctcgcg aacctggccc gccagctttt ggcggaggga gcgctttcag
     2101 cctccttcgt cccggtcttc tcaagggttc tggcggccaa ggggaaagaa agcgcggagc
     2161 gcctcgcgcg tcaggctttt tccgtgctgc tcgtcgctac gattctctcc gtcgcggcgg
     2221 gcgttgtgtt ttccccgctg ctcgtaaaga ttatggctcc ggggtttgat cctgtcaaag
     2281 ccgaattggc gacggcgatg acgcgctgga tgtttccctt ccttgtcctg gtgtcgcttg
     2341 cggcgctcgc gatgggggtg ctcaacagca tgggctcctt tctcgttccc gcgcttgccc
     2401 cggcgttgag caatctcgtc tacatcgtgc tggttgtgtt ccttgcctct ttctacggtg
     2461 tttggggcct cgtgatcgcg gtgctcgccg gtggcgcctg ccagtttctt ctccaatggg
     2521 cgtggtcggt acggatgggg gttacgctgc tccccgagcg gccgcaactg aaggatccag
     2581 atctccggac gatgctcgcc ctcttccttc cgtacgccgc cggactctcg ctgaaccagg
     2641 tgaatccggt gatcagcagg atgctcgcct cctttctcca ggagggcgcc atctctgtgc
     2701 tgaactacgc aaaccgcgtg atccagctcc ccctgggaat tttcgtcatc gcgatatcgc
     2761 aggccgtcct gccccaactc tcccgatgcc cggcggaaga taccggggag ttccgcgaca
     2821 tcatgcgcga ctcgctcagg ttcacgctct tcgtcgtctt tcccgcgacg ctgggcctgg
     2881 cgcttgtctc ggacgaaatc gtccatctgc tcttcgtcag gggagcgttc ggcgagtggg
     2941 cctggaaagg gacctcggtt tcactcgcga tgtactctct cgggcttccc ggaatggcgt
     3001 tctccacggt ggtcatgcgg gggctctacg cccgaagcct gccgcgcggg gcgcttatcg
     3061 tcactctctc cagcgtggcg gccacgctgc tcttttcgct tgcgctgatg gggccgctct
     3121 cgttcggagg cctcgcgctc gcaacgtcgc tggcgttcag cctctccggg tgggtgggcg
     3181 tccgcatact ttcccgccac ttagggatgc ggttcggtat cttctccgtc ccctggacgg
     3241 caaagatggg cgtgtcgctg gcttttacgg ggctgatcgt cgtcgcgtgg cgatggtttt
     3301 tcccgtattc gccggagtct tcagggattc tcagggctgc ctgggtcggc ggcgtctttt
     3361 gcgcagctgc gtcgggatat gccgccgtga ccgctctgct tcgcttccag gagtggcgct
     3421 ggcttttcgg cgccctccgg aggaaggacc ggcgggagac gccgggacag aatgcaaagg
     3481 aggaatgaat tatgacgttt cgtaagctgc ttccgtgtgt gtgtgtactc gcgctgctcc
     3541 ttccggtaca gtcgtttgcg ggagatgtgg agtcgctcct cgaaaagaga agcgcgattc
     3601 tctggttcga gggcgaagag ttcggtgaac tcattattgg cgccagggcg cagttcgcct
     3661 tcgtctacgt cgacggcgcg ctctcagagg ccgtggtgaa agagccgggc gctcccgact
     3721 ggctgcggga caacatctac tactacgggt cgaaggagac gcggaagaaa gcgctcttca
     3781 tcgtccggct ccgaacggta aagaatctcg atcttcagct ctcgatgatc aagatcggca
     3841 cgcacacgct cacgccggag gatgtgctca cgaacaaaca ctatgtgccg gtaggggagc
     3901 t
//
LOCUS       DAOSWW010000130         3887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16309_length_3887_cov_3.912317, whole genome shotgun sequence.
ACCESSION   DAOSWW010000130 DAOSWW010000000
VERSION     DAOSWW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16309_length_3887_cov_3.912317"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..784)
                     /gene="tsaB"
                     /locus_tag="PK442_03815"
     CDS             complement(107..784)
                     /gene="tsaB"
                     /locus_tag="PK442_03815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOO86806.1"
                     /translation="MKEYTLSVDCCSAWTTLGLAEDEKVRGEINIDAGKNQSALLPGL
                     LQHFLKAFSLTPEDISLFSVVTGPGSFTGIKVGISFVTFLAWAAGKSIVPLSSLECMA
                     FEKIRRSGGLAASVLWGGGGKVYGGLFKGEGDTLPPLSLFRSGSFTPELFLEAFSGTK
                     LRHQDVFWLTDAPEKVAPLFPSFGGSFEKIIPTGSATVELTRRHKGRARSAFEIHADY
                     FRDPDLG"
     gene            complement(1516..2379)
                     /locus_tag="PK442_03820"
     CDS             complement(1516..2379)
                     /locus_tag="PK442_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOO86807.1"
                     /translation="MSSFSAFLPQRPASIHKGDRGGVLIVGGSSLYRGAPVLAARGAL
                     RSGCGLAVIFSEEVVCAGAASSLPECIVQAGLFTSSRKEIINVLESWESRIECLVLGP
                     GLGRSPEAGKIVRLVLEKWEKSMILDGDGIFWLSQYAGFFERRASLLLTPHEGEAACL
                     LGASIESVRCARGESARRIAVRDGIVLLKGPQTLVDDGVRCEMIDEGNQALAIPGSGD
                     VLSGSIAAFSAAGLPLFESACLGAVVHGRAGRLVSRKLGIDGVLASEVADALPLILQK
                     MRMRERETKTN"
     gene            complement(2376..2753)
                     /gene="acpS"
                     /locus_tag="PK442_03825"
     CDS             complement(2376..2753)
                     /gene="acpS"
                     /locus_tag="PK442_03825"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOO86808.1"
                     /translation="MIQGIGVDLCSISRMEKAVESAHFIHRVFSPEEIQYACGKARPA
                     RHFAGCFAAREAFSKASGISMYSLAFGGGMWIERTGSAPIIRCSHTVLDLLPHKGTER
                     IHLSLSHDGDYAVAMVVLEIGFQ"
     gene            complement(2811..>3887)
                     /locus_tag="PK442_03830"
     CDS             complement(2811..>3887)
                     /locus_tag="PK442_03830"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86809.1"
                     /translation="KKESPQRAEKVKVHSAWRVRRFFTWFFFFLSVLMLGAGAFFFFA
                     ASDLMERAKAGDASAQRSIGRLYEIGGILGSDQKAAEWYLEAAKQGDASAQFALGRLY
                     SAGALGAPDFTAAAEWYEKAAELGHVNAQFALALSFENGRGRARDDALAVRWYRRAAE
                     QGDVRAQFNLGNMAEQGRGMNQSDTQAADWYHRAGSQGHTEAQLRLARMYEQGRGVSQ
                     SEDSAARWYRSAAESGEVSAQFIVGTMYEEGRGVTPDPAQAAKWYRKAAEQGNAAAQY
                     SLGRLYTNGLGGKQDDVEAASLYMKAAEQGYAPAQFSLGWSYEFGRGVFRNEARAAEW
                     YRRAAAQGHTLAHIALDRLLSGDE"
BASE COUNT          937 a         1145 c          919 g          886 t
ORIGIN      
        1 tgctcttctg atgagtagga tagcttgaac ctcaacacag ttcaacatca aatataataa
       61 tttctttttt atctcttcga ttttctaaag aaaaaaatga taaaatctat ccgaggtctg
      121 gatccctaaa ataatcggcg tgtatttcga aagcagaacg tgcacggcct ttgtggcgcc
      181 gcgtcaattc gactgtcgcg gaaccggttg gaatgatttt ttcgaaggac ccgccgaaag
      241 aagggaaaag aggggctact ttctcaggag cgtctgtgag ccaaaagaca tcttgatgac
      301 gaagcttcgt cccggaaaaa gcttcgagga ataactccgg agtaaaggat ccgctgcgga
      361 aaagactgag cggaggaagc gtgtctcctt ctcctttgaa aagaccgcca tacactttcc
      421 cacctcctcc ccaaagtacc gaagcagcca gaccgccgct tcttctgatc ttttcgaagg
      481 ccatgcattc aagcgaagac aacggaacaa tactttttcc cgcagcccac gcaagaaagg
      541 tcacgaaaga aattccaacc ttgattccgg tgaaggaacc ggggccggtg acaacgctga
      601 aaagagagat gtcctccggt gtgagggaga atgctttcag aaagtgttga aggagccccg
      661 gaaggagagc cgactgattt tttccggcat cgatgttgat ctctccgcgt accttctcgt
      721 cttccgccag cccgagtgtg gtccatgccg agcagcagtc aactgaaaga gtgtattctt
      781 tcattatttt ttcttcctct ccttgagttt tcccagaaaa gattccaggg ctttctctgc
      841 gcgggcgcct tgggccgaaa aagagagcac tcttcgtccc tcgtcctctt cttcgtcttc
      901 atatcgaaaa cgaattctcc atatttcgct gaagtctccg ggtacccctc ggtctgccca
      961 ttccacgaag agaacactcc ggtcatccaa ataactctct acatcgagtt cggtaggatc
     1021 gaccgaagac aggcgataaa ggtcggagtg cactacggtt ttctctccgc acgaataacg
     1081 attaaccagt gtaaatgaag ggctgcgcac gccgatgtat ccgtctgccc ggcaaaaccc
     1141 tcttacaagc gtcgtttttc ctgttcccaa atcgccctcc aggagaaata caagtcccgg
     1201 aaaggagtgt tccgccataa gctctccaat ttgcatggtg ctttcctcgg aaaaagagat
     1261 gtattcacag catgagatcg aatccatcgg ttaagagtcc cctatcattt ctgttttctc
     1321 cgcatcaaca tgataaaaag aagagtcccg gcggccacaa agagaagcac caaaattaga
     1381 ggtcccatgg aaagactctt tcttcccatt cgtcctacgc cgaaaatcca gaacgcggca
     1441 agcgcaaaac tcgcgatgct gaagcgaaga cctttccagc gaacttgttc ggtttttcta
     1501 atttcttcat cccagtcaat tcgttttcgt ctcacgttct ctcatcctca ttttctgaag
     1561 gatcagcgga agagcgtcgg cgacttccga agctaacact ccgtcgatcc ctagttttcg
     1621 tgatacgagc cttccggcgc gtccatggac tacagcgccg aggcatgcgc tctcgaagag
     1681 cggcaaccct gcggccgaaa aggctgcaat ggagccggaa agaacatctc ctgaaccagg
     1741 aatcgcaagc gcctggtttc cctcgtcaat catttcgcag cggactccat catctacaag
     1801 cgtttgcggt cctttcaaaa gaacaatacc gtcccggacg gcgatccttc gggcggattc
     1861 accacgagcg caacggaccg attcgatcga agcgccaaga aggcacgctg cttctccttc
     1921 gtgcggagtc aggagaagag aagcgcgacg ttcaaaaaaa cctgcatact gcgaaagcca
     1981 aaagatgccg tctccatcaa gaatcatgga tttttcccac ttttccagta caaggcggac
     2041 aattttcccg gcttccggtg atcttccaag acccgggcca agcaccaggc attctatacg
     2101 ggattcccag gattccaaga cattgattat ctcttttcgt gaggaagtga aaagaccggc
     2161 ctgaactatg cattcaggaa gagaagacgc agcgccggcg caaacgactt cctcagaaaa
     2221 aataaccgct agaccgcatc cggagcgaag cgctccccgc gccgcgagga ctggcgctcc
     2281 tctgtacagt gaactgcccc cgacgataag caccccgcct ctgtcgcctt tatgaatact
     2341 cgccggcctt tggggaagga atgcggaaaa actgctcatt ggaagcctat ctccaggacg
     2401 accatcgcga ccgcatagtc tccgtcatga gagagcgaga ggtggatcct ttccgttcct
     2461 ttgtgcggca acagatccag gacggtatga gaacatctga tgatcggagc ggatccagta
     2521 cgctcaatcc acatgccgcc accgaaagcc agggaataca tggaaatgcc agaggccttc
     2581 gaaaaggctt cgcgcgcggc aaaacagcct gcaaaatggc gtgctggacg agccttcccg
     2641 caagcgtact gaatctcctc aggggagaaa accctgtgaa tgaagtgtgc ggattctacg
     2701 gccttttcca tccgtgaaat gctgcataaa tcaacaccta ttccttgaat catattctac
     2761 ctccaaaaac gccttagaaa attgagttcg acgacctcat aaagtgaaga ttattcatct
     2821 cccgaaagaa gccggtcaag agcgatgtgc gccagcgtgt gaccctgtgc ggcggctctc
     2881 ctgtaccatt cggccgccct ggcctcgttt cggaacactc ccctcccgaa ctcatagctc
     2941 caccccaaac tgaactgcgc gggagcgtac ccttgttccg cagccttcat gtacaaagaa
     3001 gcggcctcga catcgtcctg cttcccgcct agtccgttcg tgtacaatcg ccccaggctg
     3061 tactgagccg ccgcgttccc ctgctcagca gcctttctat accactttgc ggcttgagca
     3121 ggatcaggtg tgactccccg cccctcttca tacatagtac cgacgataaa ctgggcggag
     3181 acctctcccg attctgcagc gctccggtac cagcgtgccg ctgaatcttc gctctgagaa
     3241 acgccgcgtc cctgctcata catccttgct agtctgagct gcgcttcagt gtgcccctgc
     3301 gaccctgccc tgtgatacca gtcggcggcc tgtgtatcgc tttgattcat gccgcgtccc
     3361 tgttccgcca tgtttccaag gttgaactgc gcgcgtacat ctccttgttc tgctgctcta
     3421 cggtaccatc gaacagccag agcatcatcc cgcgctcttc cccgtccgtt ttcgaaagag
     3481 agcgcgagag cgaactgggc gttgacatga ccgagttccg ccgctttttc gtaccactcc
     3541 gccgctgccg taaaatcagg agctcccagc gctcctgcgg aatacagtct tccaagggcg
     3601 aactgggcag aggcatctcc ctgtttcgcg gcttccaaat accattccgc agccttctgg
     3661 tcgctcccga ggattccccc gatctcgtac agccttccga tgctcctttg tgcggaagcg
     3721 tcccccgctt ttgcccgttc catcaggtcg cttgccgcga aaaagaaaaa ggcccccgct
     3781 ccgagcatga gcaccgaaag gaagaaaaag aaccatgtga aaaaccttcg tactctccac
     3841 gccgaatgca ccttcacctt ctctgcgcgc tgcggacttt ctttctt
//
LOCUS       DAOSWW010000131         3887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16313_length_3887_cov_2.996868, whole genome shotgun sequence.
ACCESSION   DAOSWW010000131 DAOSWW010000000
VERSION     DAOSWW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16313_length_3887_cov_2.996868"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..995)
                     /locus_tag="PK442_03835"
     CDS             complement(204..995)
                     /locus_tag="PK442_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO86810.1"
                     /translation="MDMFLYIDKETPVHRLDPRTKLLLMLLSFVLALSFRNFAILSSV
                     LVGVLLYGAAGGALSNLRRIRVVLLMIALFSMGIWSVATPSDSRFLIFSLEGLTYGVM
                     VAIKTDLMIISGMIFLSTTKIEEIAGGLVKLKLPYRGAFAFSTAIRLVPMIVATSYTI
                     IQAQKSRGLDLDSGSMLQRARKYVPLVIPTLVSVIRGTNVFSMALESKGFGYSDTRTN
                     LLELRMKRNDWLTLGVALLVVAAAFFFNMDSVAYGACLFSNIAGS"
     gene            complement(995..2707)
                     /locus_tag="PK442_03840"
     CDS             complement(995..2707)
                     /locus_tag="PK442_03840"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOO86811.1"
                     /translation="MNDEKAIVVRGLTFSYRGDEDVRAVKSLDFEVDRGEFVVIMGPS
                     GAGKSTLANSLNGLIPRFLRGKYEGSVLVNGLDAAKHSVSRMADEIGLVFQDFEAQLF
                     STNVKLEVAFGPENFAVPREEIAERIGRVLKTVNLEGVEGRSPATLSGGQKQRLAIGS
                     VLAMHPDIVCMDEPTTDLDPLGKMGVFAIAKELHEQGALTLLIIEHETEEALHADRLV
                     LMKDGEIAAQGKPGDILRDVPFTDGVGIMPLQIPKYFFELKLCEREELPLLPKDGAEF
                     FRRKGLVLEQSRYKWLIEGDRARERHYGKTIVKAEGVTHVYPDGNRAVDDVGLEIRER
                     EFVAILGHNGSGKTTLVKHFNGLLQPTSGKVTVCGKNTAEHSIYEIGKEIGYVFQNPD
                     HQIFAETVFEEVAFSPKIRGCPEEELAVRVREALEAVDLAGYEKKDPFSLSKGERQRV
                     AVASVLSARPGIIILDEPTTGLDYTEQRKMMELVRKLNESGQTVIMITHTMWVVAEYA
                     HRVVVVKDGRVLLDGATRDVFAREPELEASHLRPPHIVSMSNLLGHTLLSVEEMKYCT
                     GGVS"
     gene            2975..3874
                     /locus_tag="PK442_03845"
     CDS             2975..3874
                     /locus_tag="PK442_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO86812.1"
                     /translation="MYLAGVLYCCAAAVCWALGPVFLKKGLALMSHSEMGAARTFGFV
                     GAALFFVMLEPGVAVGWNYPLPYLAVIFVSILIGNIVGDLAYFRSIEMIGVGRAVGTT
                     SCYPLFVTAISSVWLGEAVTLPLVLGTLVMIAGLVLLKSGGKQSAETRSVSGAGFFFA
                     IVAAVSWAGMMVLQKWLISVHSVHPASITLWRSFFLFSLSWLYWGTSSLRRGTNPMRI
                     FAMSPKAWGWAIAAGTFGLAAGGYFFTKGIQLIPVSIATPITASSPLLAAAMGVLFFH
                     ERMRPVQWAGILCILSGVLAVNS"
BASE COUNT          703 a         1317 c         1072 g          795 t
ORIGIN      
        1 tgagcacgaa ccctttgaaa gggatcagca gggctgcgta caggccggcg cagagcgcca
       61 caagcactat catcttcgtt ttcttccaca tcgagaatac ttcgttcaca aagaccactc
      121 cttcaaggtt tgactccgcg tttcgccata tccgccgcta aggccgcggc gctccgccct
      181 cgctccgcaa cgatgggcgc gcttcagctc cccgcaatgt tactgaaaag acacgctccg
      241 tacgctaccg aatccatatt gaagaaaaac gccgccgcga cgacaagcag cgcgacgccc
      301 agcgtcagcc agtcgttccg cttcatccgc agttcgagaa gattcgtccg cgtgtcgctg
      361 tatccgaacc ccttcgactc cagcgccatc gagaagacgt tcgtcccccg gatcaccgaa
      421 accagcgtgg gaatgaccag cggcacgtac ttgcgcgccc gctggagcat cgatcccgaa
      481 tcaaggtcaa gcccgcgcga cttctgcgcc tggatgatcg tgtagcttgt ggccacgatc
      541 atcggcacaa ggcggatcgc ggtggagaac gcgaacgccc cccggtacgg cagcttcagc
      601 ttcaccagac ccccggcgat ctcttcgatc ttcgtcgtgc tcaggaagat catccccgag
      661 atgatcatca aatcggtctt gatcgccacc atcacgccat aggtcagccc ttcgagggag
      721 aagatcagaa aacgggagtc cgagggggtg gcgaccgacc agatacccat ggagaagagc
      781 gcgatcatca gcagcacgac gcgtatccgc cgcaggttgg agagcgcgcc ccccgccgcg
      841 ccgtagagca gcacgccgac gagcaccgac gacagaatag cgaagttcct gaacgagagc
      901 gccaggacga aactgaggag catcagcagc agcttcgtcc gcgggtccag ccggtgaacg
      961 ggcgtctctt tgtcgatgta gaggaacata tccatcacga aacgcctccc gtgcagtact
     1021 tcatctcctc caccgagagc agcgtatgcc cgagcagatt gctcatcgag acgatgtgcg
     1081 gcggccgcag gtgcgacgcc tccagctccg gctctctggc gaagacgtcg cgggtcgccc
     1141 cgtcgagcag cacccgcccg tccttcacca cgaccaccct gtgggcgtac tccgccacca
     1201 cccacatcgt atgcgtgatc atgatcaccg tctgccccga ctcgttgagc ttccgtacca
     1261 gctccatcat cttccgctgc tccgtgtagt cgagccctgt ggttggttcg tcgaggatga
     1321 tgattcccgg ccgcgccgag agcacggaag cgacggcgac gcgctgtcgc tctcccttgc
     1381 tcagcgaaaa ggggtccttc ttctcgtatc ccgcgaggtc caccgcttcg agcgcctcgc
     1441 gcaccctgac ggcgagctcc tcctccggac accccctgat cttcggactg aacgcaacct
     1501 cttcgaagac ggtctcggcg aatatctggt ggtccggatt ctgaaagacg tacccgatct
     1561 ccttgccgat ctcgtagatc gagtgctccg cagtattctt cccgcagacc gtcaccttgc
     1621 cggaggtggg ctgcaacagg ccgttgaagt gcttcaccag cgtcgtctta ccgctgccgt
     1681 tgtgccccag tatggccacg aactcccgct ccctgatctc caggccgacg tcgtccaccg
     1741 ccctgttgcc gtccgggtag acgtgcgtca ccccctcggc cttcacgatc gtcttcccgt
     1801 aatgccgctc ccgcgcgcgg tctccctcaa tgagccactt gtagcgggac tgttccagaa
     1861 caagcccctt tcgccggaag aactccgccc catccttcgg gagcagcggc agttcttccc
     1921 gttcgcagag cttcagctcg aaaaaatact tcggaatctg cagaggcatg atgcccactc
     1981 catcggtaaa cggcacgtcg cgcaggatgt cgcccggctt cccctgcgcc gcgatttccc
     2041 cgtccttcat cagcacgagg cggtcggcgt gcagggcctc ctccgtctcg tgctcgatga
     2101 tgagcagcgt gagggccccc tgttcgtgca gctccttcgc gatggcgaac acccccatct
     2161 tccccagcgg atcgaggtcg gtggtcggct cgtccataca gacgatatcg gggtgcatcg
     2221 cgaggaccga gccgatggca aggcgctgct tctgcccgcc ggagagcgtc gcggggctgc
     2281 gcccctccac tccttcaaga ttcacagtct tcagcaccct gccgatgcgc tccgcgattt
     2341 cctcccgggg gaccgcgaaa ttctccgggc cgaacgcgac ttcgagcttt acgttggtcg
     2401 aaaagagctg cgcctcgaag tcctgaaaca caagacctat ctcgtcggcc atccggctga
     2461 cggagtgttt cgccgcatcc agaccgttta cgagcacgct tccctcgtac ttcccgcgca
     2521 gaaaacgggg gatcagcccg ttcaggctgt tcgccagagt cgacttcccc gcgccgctcg
     2581 gtcccatgat cacgacgaac tcgcccctgt ccacttcgaa atcgagcgac ttcaccgccc
     2641 ggacgtcctc gtcccctctg taggaaaaag taagccctcg taccactatg gctttttcat
     2701 cattcattac ctaaatcccc gcccctttcg gtcggtagcg ctcttggaat gcgcacatcg
     2761 gccgcgtttc cttcgcagga gacacgaatg cgccaccccg gattatacca gagggggccg
     2821 gctctttccc tgaaaaaccg aagggaaccg actccctttt tcacagagga cccggtctcc
     2881 actctctttc gcacgaaaag aagtttctgt gctatcattg cgtgcaatgt cacatcgata
     2941 ttccatggtc cgccggaccg aggaggctac tcaagtgtat cttgcaggtg ttctgtattg
     3001 ctgcgccgca gctgtgtgct gggctctcgg gcctgttttt ctgaagaaag ggctcgcgtt
     3061 gatgagccat tcggaaatgg gagcggcgcg aaccttcggc ttcgtcggcg cggcgctgtt
     3121 tttcgtcatg ctggaacccg gcgtggcagt cgggtggaac tatccccttc cctatcttgc
     3181 cgtcatcttc gtcagcatcc tcatcggcaa catcgtcggc gacctcgcct acttccgctc
     3241 gatcgagatg atcggcgtcg gcagggctgt gggaaccact tcctgctacc ccctcttcgt
     3301 caccgcaatc tcctcggtct ggctcggcga agccgtcacg ctccccctcg tgctgggtac
     3361 tctggtgatg atcgccgggc tcgttttgct gaaatcgggg gggaaacagt ctgcggaaac
     3421 gcgctcagtc tccggggcgg gcttcttttt cgccattgtc gcggcagtat cctgggcggg
     3481 gatgatggta ctccagaagt ggctcatatc ggtccactcc gtccaccctg cctccatcac
     3541 cctctggcgc tccttctttc tcttctctct ctcatggctc tattggggaa caagttccct
     3601 gcgccgggga acaaacccga tgcggatctt cgccatgagc ccgaaggcct ggggctgggc
     3661 aatcgccgca ggaacgttcg ggcttgccgc gggggggtac ttcttcacga aggggattca
     3721 gctcatcccg gtctccatcg ccacgccgat cacggcgagc agcccgctgc tggccgccgc
     3781 gatgggcgtt ctcttcttcc acgagcggat gcgccccgtc cagtgggcgg gcatcctctg
     3841 catcctgagc ggcgtcctcg cggtgaattc gtaggggacc tcagaga
//
LOCUS       DAOSWW010000132         3881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16350_length_3881_cov_4.133298, whole genome shotgun sequence.
ACCESSION   DAOSWW010000132 DAOSWW010000000
VERSION     DAOSWW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16350_length_3881_cov_4.133298"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK442_03850"
     CDS             <1..181
                     /locus_tag="PK442_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86813.1"
                     /translation="LVEASKDRIQKTVSALKEYAPELVAAGHCTGFRAQAALYAAFDE
                     KFATMGAGAVFAFKA"
     gene            complement(345..1655)
                     /locus_tag="PK442_03855"
     CDS             complement(345..1655)
                     /locus_tag="PK442_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86814.1"
                     /translation="MKQTERPSFMPWKTQYSRCATETPRPSPTQKNTAAEESVFSVAA
                     LCAETLKALHQRIRKRKPRIELDIDSSIPRLARGNGAQIENILHLLLSNVIALSLDAA
                     VKLSVVTRRFTAEGLEVLFSIEWAHSGVALSADPVIEGNAPPGLSYSICSSVIAAMGG
                     RLHVKKQEENMVISFFIPLSTAGISEDAEKGGAPVLLVEDNAMNRKIIASSLEHGGYS
                     VFTASSGQEALNALFDKEWSIILMDIQMPGMDGIQTTHAIRAEEKKKGRRTPIVAVTA
                     YARPEDRERCLAAGMDDFLAKPVKLETLLSTVERVTGIPSARKSAVRGPDAASAILSF
                     TEGDAELARKIIGEFLALAPPLLLSMRDAFDRKDRETLRVLSHRLKGNLAYFGEGEAF
                     LLAEALHRELDNLSDEAVAATLLRLLSESEYLIEALENKEEAAR"
     gene            complement(1652..2377)
                     /locus_tag="PK442_03860"
     CDS             complement(1652..2377)
                     /locus_tag="PK442_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO86815.1"
                     /translation="MTKRRLPVRVLVAEDDPMVCSLHVQTIQQVHGFTVAGTVGDGIE
                     MVRFFESSSADLIVMDIFMPRLSGLDALKKLRAEGNVADVILVSAGKHPQLVSQAKLL
                     GAFDYLIKPYSLKRFRTSLEAYLENRTKMPSETEEATQETLDSLFEIASANRSPGGSE
                     KLPKGLQPGTLSLVMKELTDDKGLSAEEICTRLSISRSTAWRYLDHLSKTGAVRIQFE
                     YNGPGRPLKRYLLRESIRSELLV"
     gene            2687..2857
                     /locus_tag="PK442_03865"
     CDS             2687..2857
                     /locus_tag="PK442_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86816.1"
                     /translation="MTNFAQSVAPYILYTCSLVLLPLGVNRFRAGSPVKGFLDFVGAG
                     VIAVMAWSLANP"
     gene            2977..>3881
                     /locus_tag="PK442_03870"
     CDS             2977..>3881
                     /locus_tag="PK442_03870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO86817.1"
                     /translation="MNRFRKVCVSSLLLLVLLASVAVATETKPEPAKAEPSPAAVEEK
                     APVADPETVLATVGSAEIKQKDVTAIIGRLDPQRAAMYDNEMGRRAILEELVNMELFS
                     LLGAELAVEKDPKFIETMNEVRKDVTRQFAVEAIMKDVAVTDEEIAAYYEKNKENFKV
                     PETVRASHILVAGEDEMKKVRADLDAGMSFEDAARKHSSCPSKEQGGDLGYFSKEQMV
                     PEFGNAAYAMKVGDISAEPVKTQFGLHLIKLVDKKEASVRPLDEVRAQLTESLLNEKK
                     AGVYNEQLAKLREKHQVVIVGEKKE"
BASE COUNT          819 a         1035 c         1138 g          889 t
ORIGIN      
        1 cctcgtggag gcgtcgaaag atcgcattca gaagacggtt tcggcgctga aggagtacgc
       61 tcctgaactt gtcgctgcgg ggcactgtac agggttccgc gcacaggcgg cgctctacgc
      121 ggcgttcgat gaaaaattcg caactatggg agccggcgcc gtcttcgcct tcaaggcgta
      181 atggcctgtt ccattggaaa aaatgtaccg tgctgcttcc gactgtcgcg ggactgatcg
      241 cagggaagct tgcgggcggt atgaggaagg gctagaccaa aaagccggtt gtagatgata
      301 ccagccggca ggtaataaag aaaagcaagg ggaagcgtcc gaagttatcg ggctgcttcc
      361 tctttgtttt ccagtgcttc aataaggtat tcgctctcag aaagaaggcg gagcaaggtt
      421 gccgcgacgg cttcgtcgga gaggttgtcg agctcccggt gaagcgcttc cgcaaggaga
      481 aaggcttctc cttcgccgaa ataggccaga ttccccttga gtctgtgaga gagcacacgg
      541 agcgtctccc tatccttccg gtcgaaggca tcgcgcatcg agagcagcag cggcggggcg
      601 agcgcgagga actcgccgat gattttccgc gccagctcgg catccccttc cgtaaaggag
      661 agaatggccg aggcggcgtc cggtcctctt accgcgctct ttctggcgga ggggatgccg
      721 gtgactctct cgacggtgga gagaagcgtc tccagtttga ccggttttgc gaggaagtcg
      781 tccatccccg cggcaaggca gcgctctcta tcctcgggtc tggcgtacgc ggtgacggcg
      841 actatcggag tgcgccgccc ctttttcttc tcctcggcgc ggatggcgtg agtcgtctgt
      901 atcccgtcca ttcctggcat ctggatatcc atcagaatga tgctccactc tttgtcgaag
      961 agggcgttaa gcgcttcctg accggaagaa gcggtgaaaa ccgagtaccc tccgtgttcg
     1021 aggctggacg cgatgatctt cctgttcatc gcgttgtcct ccacgagcag aaccggcgcg
     1081 ccgcctttct ccgcatcctc ggagataccg gcggttgata acggaataaa aaacgagatg
     1141 accatgttct cttcctgttt tttcacgtgc aaacggccgc ccattgcagc gatgaccgac
     1201 gagcaaattg aataactcag gccgggcggc gcgtttcctt caataacggg atcggccgac
     1261 aaagcaacgc cgctgtgtgc ccattcgatg gaaaaaagaa cttccagccc ctcggctgta
     1321 aaccttcgtg tgacaacgga gagttttacg gcggcgtcca aactcaacgc gatgacgttc
     1381 gagagaagaa ggtgaagtat gttctctatc tgcgctccat ttcccctcgc caaacggggg
     1441 atggaggagt cgatgtcgag ctcgatgcgc ggcttccttt ttcgaatgcg ttgatgaagc
     1501 gctttcaggg tttcggcaca gagagcggcg acagaaaaaa cgctctcttc cgccgcggta
     1561 tttttttgtg tgggcgaggg gcgcggcgtc tcggttgcac accggctgta ctgggtcttc
     1621 cacggcataa aggatggacg ttcggtctgt ttcatacaag taactccgat ctgatgctct
     1681 cgcgcaggag atagcgtttc agcggccttc cggggccgtt gtattcgaac tggattctga
     1741 cggcgcctgt tttggagagg tggtcgaggt acctccatgc ggtggaacgt gagatagaaa
     1801 gacgagtaca tatctcttca gcagaaaggc ctttgtcgtc ggtgagctct ttcattacaa
     1861 gggagagcgt tcccggctgc agccccttgg gcagcttctc gctccccccg ggagatctgt
     1921 ttgcggaggc aatttcgaag agagagtcca gcgtctcctg ggtcgcctcc tcagtctccg
     1981 acggcatctt tgtcctgttt tcaaggtagg cttcaagaga tgtccggaag cgtttcagcg
     2041 aatagggctt gatcaggtag tcgaacgctc caagcagctt ggcctgggat accagttgcg
     2101 gatgcttgcc ggccgataca agaatgacgt cggcgacgtt cccctcggcg cggagttttt
     2161 tcagcgcgtc gagaccggaa agacggggca tgaaaatatc catgacgata agatcggctg
     2221 aagacgactc gaaaaaacgc accatctcaa tgccgtcgcc gacggttcca gctacggtaa
     2281 agccgtgcac ttgctgaatc gtctgtacat gaaggctgca taccatcgga tcgtcctcgg
     2341 ctacgagaac tctgacgggt agtcttcgtt tcgtcatttt cctcacccct tgattgaatt
     2401 atatgctctc gctccgcgtt cgtaaacaca atgaacaaaa agaaaatcaa tgaacaaaac
     2461 tcttgctttt tttaggtttg tgattcagaa tgaatggtac ataaattgca ttcccggttc
     2521 ggttgatgaa cttccccacg cactggttcc gcttcccgaa aacctgcccc gggttttcga
     2581 cgaggagcgg aacccccttt tttctctctg gcttcggaaa cgctcatccc gcttgacttt
     2641 cttctccatg cactgcatta ttaacacgtc caaaggaggg gtctcgatga cgaattttgc
     2701 gcagtcagtc gcgccgtaca tcctgtatac gtgttcgctc gttcttcttc cgctcggggt
     2761 caatcgcttc agggcgggca gtcctgtgaa ggggtttctc gatttcgtcg gcgcgggggt
     2821 cattgccgtc atggcctgga gtcttgcgaa cccctaggcg ctgatggcat gatacggggt
     2881 ttcagcattt tgtacgtttc tttttttctt ttctatggta cactctgtcc gttttgttcc
     2941 aaattccgtg aaggattatt ttagggaggt ttttgaatga atcgtttcag aaaagtatgc
     3001 gtgtcatcgc tgttgctcct tgttcttctg gcgtccgtcg cagttgcgac cgagacgaag
     3061 ccggaaccgg caaaggcgga gccgtctcct gcggcagtcg aagagaaggc cccggttgct
     3121 gatcccgaga ccgtgctcgc tactgtcgga tcggccgaga tcaagcagaa ggatgttacc
     3181 gcgattatcg gccgtctcga tccccagagg gcggccatgt acgacaacga gatggggcgc
     3241 agagcgattc tcgaggaact ggtcaacatg gagctcttct ctctgctcgg ggccgagctc
     3301 gctgtggaga aggatccgaa gttcatcgag accatgaacg aagtccggaa ggacgtcacg
     3361 cgccagttcg cagtcgaggc gatcatgaaa gacgtcgccg tcaccgacga ggagatcgcc
     3421 gcgtactacg agaagaataa ggagaacttt aaggttcccg agaccgtccg cgcgagccat
     3481 attctggtcg ccggcgagga cgagatgaag aaggtgcgcg cggatctcga cgcgggaatg
     3541 tccttcgagg atgctgcgcg gaaacactct tcctgccctt cgaaggagca gggcggcgac
     3601 ctgggctact tctccaagga gcagatggtg ccggagtttg gcaacgccgc ctacgccatg
     3661 aaggtcggcg acatctctgc cgaacctgta aagacgcagt ttggtctgca tctgatcaag
     3721 cttgtcgata agaaagaggc ctccgtacgc cccctcgacg aagtccgggc gcagcttacc
     3781 gagtccctcc tgaacgaaaa gaaggccggc gtctacaacg aacagctggc gaagctccgt
     3841 gagaagcacc aggttgtcat cgtgggagag aagaaagaag a
//
LOCUS       DAOSWW010000133         3876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16381_length_3876_cov_3.368490, whole genome shotgun sequence.
ACCESSION   DAOSWW010000133 DAOSWW010000000
VERSION     DAOSWW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16381_length_3876_cov_3.368490"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PK442_03875"
     CDS             <1..898
                     /locus_tag="PK442_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO86818.1"
                     /translation="TVLVNASGLFAADVCRMAGITEYSIYPCRGEYYVLDKRLDGTLR
                     ALIYPAPKKNAPGLGIHLTPTVDGNILIGPSAEYLNDPEDHSCTYDVMAELRREGKEL
                     LGEIRMTDYIRNFAGIRPKQTPPEVGGNKDFVIEDRKDAKGFVNLVGIESPGLTSAPA
                     IAEMVRGMVEKHIPLLPDPAFNPVRPGSPFFFSELPADERAAMIAENPNYGEIICRCE
                     KITKQEILDAIKNPLGARTLVSIKYRARASMGRCQGGFCVPRITRILRDEFGWNPEDF
                     LKRGGSSPMFVGNVRPKGGVSR"
     gene            895..2157
                     /locus_tag="PK442_03880"
     CDS             895..2157
                     /locus_tag="PK442_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO86819.1"
                     /translation="MNVKTSALETTKRDVVVIGGGPAGLAAAIAAKEAGCGDVLLIER
                     DRILGGILNQCIHDGFGLHTFKEALSGPEYAARYIDRFHELGIEAMEETIVLSLTPDR
                     VVEVSTRGTLRRIRAGAVILAMGCRERTRGAISIPGNRPSGVYTAGAAQNFMNLENIM
                     VGRKVCILGSGDIGLIMARRMTLEGASVEAVFEILPYSSGLPRNIQQCLNDYGIPLFL
                     NTTVTEIVGKGRLEGIRVASVGEDRAPIPDTERFIACDTLLLSVGLIPENELSRDAGV
                     AMDPVTGGAVVNDSFMTSVDGIFACGNVLHVHDLVDWVSVEAAEAGKFAAGYVRSGRE
                     ASSCRIPVRPGSGVRYTLPQSVSGERDCILSLRVAAPWRNRCIVVKSGEREVARKKEM
                     RLHPAEMIRIPLKKEALSGCSSLEVTVE"
     gene            2154..2546
                     /locus_tag="PK442_03885"
     CDS             2154..2546
                     /locus_tag="PK442_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOO86820.1"
                     /translation="MSTRELTCVVCPGGCTIVVDVDNDETPVVTRIQGNNCPRGEAWA
                     RQEVENPMRTIASSVPVRGGDFPLVSVRTLRPIPLTKIREVMEEIRTLSLEAPVEIGS
                     VVLASPAGCDTEVVATRRVKAAAPCAGA"
     gene            2610..3620
                     /locus_tag="PK442_03890"
     CDS             2610..3620
                     /locus_tag="PK442_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOO86821.1"
                     /translation="MKKVLIAVLVLFALCAAIGGMTSEAEAKSYRINIATATTGGAYY
                     PIGNAMAQIWSRNLEDVRRASAQSTAGTPQNVELMKDGEVQIAFGQNGICYYAYYGKG
                     IYDGKPPYTDLRGMFTLYPNVMHWLVRKGSGIQSIADFKERKFVPGQVASATEINSRE
                     MMEAYGLNYMKDAGEVNVTADFVGYHEAADLLKNAQTDGTHIAGGLPTAAIMDALSSG
                     VAEMISIEEDKIKEICEKYPWYFPFTIPAGTYPNQEKEIKTLALANILFTDAKQDEEL
                     IYMLTKATYDYHEDLVNAHRATAYTVLENSLKGMTVPLHPGSVKYFKEMGVDVPEQLI
                     VK"
     gene            3686..>3876
                     /locus_tag="PK442_03895"
     CDS             3686..>3876
                     /locus_tag="PK442_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86822.1"
                     /translation="MTQQNDLLEQLPELDAERAAEKVLEKADAEARFRKYGGRWAVAV
                     TVIAVAMSIFHLYTSGFGT"
BASE COUNT          819 a         1125 c         1217 g          715 t
ORIGIN      
        1 tacggttttg gtgaacgctt ccggactctt cgcggcggat gtctgcagaa tggccgggat
       61 taccgaatac tcgatctacc cctgtcgtgg agaatactac gtcctcgaca agcggctcga
      121 cgggacgctc cgcgcgctca tttacccggc gccgaaaaag aacgcgccgg gtctcggcat
      181 tcatctcacc cccacagtgg acggaaacat cctcatcggc ccgagcgccg aatacctgaa
      241 cgatccggaa gaccactcct gcacgtacga cgttatggcg gagctgcgcc gcgaggggaa
      301 ggaactcctg ggcgagatac gcatgacgga ctacatccgc aacttcgccg gtatccgccc
      361 gaagcagacg ccccccgaag tcggcggtaa taaggacttc gtcatcgaag acagaaagga
      421 cgcgaagggg ttcgtcaatc tcgtcggcat tgaaagcccc ggtttgacga gcgcccccgc
      481 gatcgccgaa atggtgcggg gaatggtgga gaagcatatc ccgctccttc ccgaccctgc
      541 gttcaacccc gtgcgtcccg gcagtccgtt cttcttctcc gaactgccgg ccgatgagcg
      601 cgcggcgatg attgcggaaa accccaacta cggcgagatc atctgcaggt gcgagaagat
      661 cacgaagcag gagattctcg acgccatcaa gaatcctctc ggcgcacgga cgctcgtaag
      721 tataaagtac cgcgcccgcg cctcgatggg gcgctgccag ggtggcttct gcgtgccgcg
      781 cattacccgg atcctgcgcg acgagttcgg ctggaatcct gaggactttc tgaagcgggg
      841 cggctcctcg cccatgttcg tcggcaacgt ccggccgaaa ggaggcgtat cccgatgaac
      901 gtgaagacga gcgcactgga gacgacgaag agagacgtcg tggttatcgg cggaggacct
      961 gcgggccttg ccgcggccat cgcggcgaaa gaggcggggt gcggcgacgt gctcctgatc
     1021 gagcgcgacc ggattctcgg cggtatcctc aaccagtgca tccacgacgg gttcgggctg
     1081 cacacgttca aggaagccct ctcgggaccg gagtatgcgg cgcggtacat cgacaggttc
     1141 cacgaactcg gcatcgaggc gatggaggag acgatcgtcc tctcgctcac tcctgaccgt
     1201 gtggtcgagg tgagcacgcg cggcacgctg cgccgcatcc gcgcgggagc ggttatcctc
     1261 gctatggggt gccgcgaacg gaccaggggc gccatctcga ttcccggaaa ccgcccgtcg
     1321 ggcgtgtaca ccgcaggcgc cgcccagaac ttcatgaacc tggagaacat catggtgggg
     1381 cggaaagtct gtattctcgg ttcgggagat atcggactga tcatggcgcg gcggatgacg
     1441 ctggaagggg cctctgtcga ggcggtcttt gaaatactgc cgtactcgag cggtctgccc
     1501 cggaacatcc agcagtgcct gaacgactac ggcatccccc tcttcttgaa taccacggtg
     1561 acggaaatcg tcggcaaggg gcgccttgag ggaatacggg tcgcctccgt cggcgaggat
     1621 cgcgcgccca tacccgatac ggaacgcttc atcgcctgcg atacgctgct cctgtccgtc
     1681 ggactgatcc cggagaacga gctctcccgg gatgcgggcg tcgcgatgga tcccgtcaca
     1741 gggggagccg tggtgaacga ctccttcatg acgtccgtgg acggtatctt cgcctgcgga
     1801 aacgtgctcc acgtccacga cctggtcgac tgggtctctg tggaggccgc ggaagcaggg
     1861 aagtttgcgg ccgggtacgt tcgcagcgga cgggaggcgt cgtcctgtcg tatcccggtg
     1921 aggccgggtt cgggcgttcg ctatacgctg ccgcagagcg tctctgggga acgcgactgt
     1981 atactttccc tcagggtggc cgccccgtgg cgcaatcggt gtatagtagt aaagagcggc
     2041 gagcgggagg ttgcccggaa gaaggagatg cgcctccacc ccgccgagat gatccgtatt
     2101 ccgctgaaga aagaagcgct ttcgggctgt tcttcgctgg aggtgacggt ggaatgagta
     2161 cccgcgagct gacctgcgtc gtctgtccgg gcggctgcac gatcgttgtc gatgtcgata
     2221 acgacgaaac gcctgtcgtt acgaggatac aggggaacaa ctgccctcgc ggagaggcgt
     2281 gggcgcgcca ggaagtggaa aaccccatga gaaccattgc gtcaagcgtt ccggtgcgtg
     2341 gaggagattt tcctctcgtc agcgtcagaa cgcttcggcc gataccgttg acgaagatac
     2401 gggaggtgat ggaggaaatc cgtacgctgt ctctggaggc tccggtggag atagggagcg
     2461 tggtgctcgc gtcccccgcc ggctgtgata ccgaggtcgt tgcgacccgg agagtgaagg
     2521 ctgcggcccc ctgcgccggg gcgtaaggca gggtgcgcat gcccgaactg aatggcgaag
     2581 aagttataca tcagaaggag ggaatcgttg tgaaaaaagt tcttattgca gttcttgtcc
     2641 tgtttgctct gtgtgcggca atcggtggta tgaccagcga ggccgaggcg aaatcgtacc
     2701 gcatcaacat cgcgaccgcc actaccggcg gagcctacta ccccatcggg aacgcgatgg
     2761 cgcagatctg gtccagaaac cttgaagacg tccgcagggc gtccgcacag tctacagcgg
     2821 gcactccgca gaatgtcgaa cttatgaagg acggcgaagt gcagatcgcc ttcggacaga
     2881 acggtatctg ctactatgca tactacggca agggaatcta cgacggtaag cccccgtaca
     2941 ccgacctccg gggtatgttc acgctctacc ccaacgtaat gcactggctc gtccggaagg
     3001 ggtccgggat tcagtcgatc gccgatttta aggagagaaa gttcgttccc ggacaggttg
     3061 caagcgcaac ggagatcaac agccgggaga tgatggaagc ctacggcctg aactatatga
     3121 aggacgcggg agaagtgaac gtaaccgccg acttcgtcgg atatcacgaa gccgccgacc
     3181 ttctgaagaa cgcccagacc gacggaacgc atatcgccgg cggccttccc acagccgcga
     3241 tcatggatgc gctctcctcc ggagtggccg aaatgatctc catcgaagag gacaagatca
     3301 aagagatctg cgagaaatac ccctggtact tccccttcac aattcctgcg ggtacctatc
     3361 cgaatcagga aaaggaaatc aagacgcttg ctcttgcgaa catccttttc acagacgcca
     3421 agcaggatga agagcttatc tacatgctca ccaaggcgac gtacgactac cacgaggatc
     3481 ttgtgaacgc gcacagggcg accgcctaca ccgttctcga gaactccttg aaaggaatga
     3541 ccgttccgct gcatccgggc tctgtgaagt atttcaaaga gatgggcgtc gacgttcctg
     3601 agcagcttat tgtgaaatag caaccgactg aaagcgggga ggaggctccg ccttctcccc
     3661 atctgtgtat tcggaggtag agagtgtgac acaacaaaac gatcttcttg agcaactccc
     3721 tgaactcgac gcggagcgcg cggctgaaaa ggtcctcgaa aaggccgacg ccgaagcgcg
     3781 cttccggaag tacggcggcc gctgggccgt cgcggtgact gttatcgctg tagccatgtc
     3841 catcttccat ttgtacacct ccggtttcgg gacgtt
//
LOCUS       DAOSWW010000134         3870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16413_length_3870_cov_3.041940, whole genome shotgun sequence.
ACCESSION   DAOSWW010000134 DAOSWW010000000
VERSION     DAOSWW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16413_length_3870_cov_3.041940"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PK442_03900"
     CDS             <1..871
                     /locus_tag="PK442_03900"
                     /inference="COORDINATES: protein motif:HMM:NF018032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HOO86823.1"
                     /translation="VEYIAPGLAPVISSLGLPLAEGKTASVDDLERLSRRMAAFFDEL
                     MGLAPRSSLFESMLTAHDFRSGDLKVKHVSFSGGVADGIAGVPDGGPFVYGDIGILLG
                     QAVKWTETYRNLDVVQAEETIRATVVGAGSHSLEISGSTIGFSDESVFPLKNLPILKL
                     TEEDERNSLGHALSEKLHWFKDSSGRQRTVAVSFRGRENPSFDEVQRIAEDLHDGLSE
                     YLTHSDKVILVVENDMAKALGYALRSRLGDGKKVVSIDGIKVENGDYIDIGKPLAKGR
                     VLPVVIKTLVFGG"
     gene            906..2255
                     /locus_tag="PK442_03905"
     CDS             906..2255
                     /locus_tag="PK442_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOO86824.1"
                     /translation="MSDILEKIRNAGVVGAGGAGFPTHVKFARACEYILLNGAECEPM
                     LEVDQQIASVHTKKLFGAMTALVELTGAKGGIFALKEKYHDAIRNVEAALPAFPKLCH
                     HVLSNSYPAGDEHVLVYEVFKRIVPEGGIPLDVGVVVLNVETLYNIASALEDRPVIEK
                     YLTVAGEVKNPATLKVPVGVSFGEVIEACGGPTVKEWVAIDGGPMMGRLVSDPQEPVS
                     KTTKGILLLPRDHPLIISKGRDMHEMMRLAKIACCHCMLCTDVCPRYLLGHKLRPDKL
                     MRLAAYNSTCEHDAAATEAFLCCECGLCETACIMGLQPWKLNKELKVRMNAAGIKNPH
                     HERPERVNPFREYRLFPVTKLTHRLGLSKYIGRKAPLREEFPSPVEKVVLKLKQHIGA
                     PAEPIVAPGDVVERGQMVARGEGPVSAHVHSSISGVVRAVEKDRIIVEATTDGRENE"
     gene            2252..2800
                     /locus_tag="PK442_03910"
     CDS             2252..2800
                     /locus_tag="PK442_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOO86825.1"
                     /translation="MSKAIGLVEYKTIPKGIEAADAMLKASEVQLLFSSPICPGKYVT
                     IVGGEVDSVKTAVDKAVAVGDIFTVDSHVLTSIHPSVLPAITATVEIKKVEALGVVET
                     ISAVAAIQAGDVAAKAGNVQLVEIRIARGLGGKGFVLFTGDLGSVQTSVRACEKKLGE
                     EGGIVSSAVIPSPHKDLISSVL"
     gene            3102..3836
                     /locus_tag="PK442_03915"
     CDS             3102..3836
                     /locus_tag="PK442_03915"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOO86826.1"
                     /translation="MWSYGLCIWTGICIASLLYWYRYGEDPRTVPSLFWGYIGIGGGF
                     FLVHWFKMLSQYGIGVFLQLRKEIWIPSDDLAIIPGLLAGGAVIVLLYRLRRIPVLET
                     MDKQAPYLAIAHGFARIGCFFAGCCYGKPAPEGWGVIFPADSPAALHYGASVAVWPTQ
                     LMESLFMFLLSALLFTLPENRRLIGYGVGYGAGRFVLEFFRGDARVGVSFFSVSQLLA
                     AGVLMGGLLLLRFRIQIECRGKFRRR"
BASE COUNT          733 a         1091 c         1269 g          777 t
ORIGIN      
        1 agtggagtac atcgcgccgg ggctcgcccc ggtgatctcc tcgctgggcc ttcctctggc
       61 cgagggaaaa accgcgtcgg tggacgattt ggagcgcctt tccaggcgca tggccgcctt
      121 cttcgacgag ctgatggggc tcgccccgcg aagttcgctt tttgagagca tgctcaccgc
      181 gcacgacttc cggagcggcg acctgaaggt aaaacacgtc tccttttcgg ggggcgtcgc
      241 cgacggcatc gccggcgttc cggacggtgg cccgttcgtc tacggcgata tcggcatcct
      301 gctcggccag gcggtgaagt ggacggagac ctaccggaat ctcgacgtcg ttcaggcgga
      361 ggagaccatc cgcgcgacgg tggtgggggc ggggagccac tcgctggaga tcagcgggag
      421 caccatcggc ttctcggacg aatcggtctt tccgctgaaa aacctcccca tcctgaagct
      481 cacggaggag gacgagcgga acagcctcgg ccacgcgctt tcggagaagc tccactggtt
      541 caaggactcc tcgggacggc agcggacggt cgccgtctcc ttccggggga gggagaaccc
      601 ctccttcgac gaggtgcagc ggatcgcgga agacctgcac gacggactgt cggagtatct
      661 gacgcactcg gacaaggtga tcctcgtcgt cgagaacgac atggcgaagg cgctgggcta
      721 cgcgctccgc agccgtctcg gggacgggaa gaaggtcgtg agcatcgacg gcatcaaggt
      781 ggagaacggc gactatatcg acataggcaa accgttggcg aaggggaggg tgcttcccgt
      841 ggtgatcaag accctcgtct tcggaggatg aacggcccgg aacggggaac agggggaggg
      901 gctcgatgag cgacatactt gagaaaatac ggaacgcggg agtcgtcggc gcgggcggag
      961 cgggatttcc cacccacgtc aagttcgccc gcgcctgcga atacatactg ctgaacggcg
     1021 cggagtgcga gccgatgctg gaggtggacc agcagatcgc ctcggtgcac acgaagaagc
     1081 ttttcggcgc gatgaccgcg ctggtggagc tgacgggggc caaggggggc atcttcgccc
     1141 tcaaggagaa gtaccacgac gccatccgca acgtggaggc ggcgcttccg gcctttccga
     1201 agctctgtca tcacgtgctc tcaaacagct atccggcggg cgacgagcat gtgctcgtct
     1261 acgaggtctt caagcgcatc gtccccgagg ggggcattcc gctcgacgtg ggcgtggtcg
     1321 tactcaacgt ggagacgctc tacaacatcg cttcggcgct ggaggaccgt cccgtgatcg
     1381 agaagtacct gacggtggcg ggcgaggtga agaatccggc gacgctcaag gttcccgtcg
     1441 gcgtctcgtt cggcgaggtc atcgaggcgt gcggcggccc gacggtgaag gagtgggtcg
     1501 ccatcgacgg cggcccgatg atgggccgtc tggtgtccga tccgcaggag cccgtgagca
     1561 agacgacgaa gggcattctg ctgcttcccc gcgatcaccc gctgataatc tcgaaggggc
     1621 gcgacatgca cgagatgatg cgtctggcga agatcgcctg ctgccactgt atgctctgca
     1681 cggacgtctg tccccgctat ctgctggggc acaagctccg cccggacaag ctgatgcgcc
     1741 tggccgccta caacagcacc tgcgagcacg acgcggcggc gacggaggcc ttcctctgct
     1801 gcgagtgcgg cctctgcgag acggcgtgca tcatgggact ccagccctgg aagctgaaca
     1861 aggagctgaa ggtccgcatg aacgctgccg gcatcaagaa tccccatcac gagcgcccag
     1921 agcgggtcaa cccgttcagg gagtaccgcc tcttcccggt gacgaagctg acgcaccgct
     1981 tgggactgtc gaagtacatc ggacggaagg cccccttgcg ggaggagttc ccctctccgg
     2041 tcgaaaaagt cgtgctgaag ctgaaacagc acatcggcgc gcccgcggaa cccatcgtgg
     2101 cccccggcga cgtcgtggaa cgcggccaga tggtcgcgcg gggggagggg cccgtttcgg
     2161 cgcacgtgca ctcgtcgatc tccggcgtcg tgcgcgcggt cgagaaggac agaatcatcg
     2221 tcgaggcaac gaccgacggg agggagaatg aatgagcaaa gccataggac tcgttgaata
     2281 caagacgata ccaaagggta tcgaggcggc ggacgccatg ctgaaggctt cggaggtgca
     2341 gctgctcttc tcgtcgccca tctgccccgg caagtacgtc accatcgtcg gcggcgaggt
     2401 ggactccgta aaaaccgccg tggacaaggc cgtcgcggtc ggcgacatct tcacggtgga
     2461 ctcgcacgtg ctcaccagca tccacccgtc ggttcttccc gccatcacgg ccaccgtgga
     2521 gattaaaaag gtggaggcgc tcggcgtggt cgagaccatc tccgcagtcg ccgcgattca
     2581 ggcgggcgac gtggcggcga aggccggcaa cgtccagctt gtagaaatcc gtatcgcgcg
     2641 cggcctgggc gggaagggat tcgtcctctt caccggcgac ctgggaagcg tgcaaacatc
     2701 ggtgcgcgcc tgcgagaaga agctcggcga agagggcggc atcgtcagct ccgccgtcat
     2761 cccctccccg cacaaggacc tgatctcctc ggtgctgtag gaaaaaatcg aattgtacct
     2821 cctggccgcg ttccgttgga gcgcggtttt tttgttatga ggatattgta cgttcagtat
     2881 atctctgttt gttctgtgaa aatggacttg tctgtgaaaa ggatgagtgc tatattattg
     2941 aaaaacaatc aaaatagata tcatgtcata ttaagtggat agagaagagg aaggtgctcg
     3001 tttcgccttt ttttctctct tgtgttgctc tccatggaaa ggggacgtgt ccgctcgtgc
     3061 atccaatcgt ctgcattttg ccttttgggg gatgaacacc tgtgtggtcc tacggcctgt
     3121 gtatatggac cggaatatgt atcgcgtcgt tgctctactg gtaccggtac ggcgaagatc
     3181 cccggacggt tccttctctt ttttgggggt atatcggcat cggcggaggt tttttcttgg
     3241 tgcactggtt caagatgctc tcgcagtacg gcattggcgt tttccttcaa ctaaggaaag
     3301 aaatctggat tccatcggac gatctggcga ttattccggg gcttcttgca ggaggcgcag
     3361 tgatcgttct actgtaccgt ttgcgaagga tccctgttct ggagacgatg gacaaacagg
     3421 cgccgtatct ggcgattgcg cacggttttg cgcgcatagg ctgtttcttc gccggctgct
     3481 gctacggaaa acccgccccg gaagggtggg gcgtcatttt tcccgcagat agccctgccg
     3541 ccctgcacta cggcgcatcc gtcgccgtat ggccgacgca gcttatggag tctcttttca
     3601 tgtttctgct gtcggcgctg ctctttacgc tgccggaaaa ccggaggttg atcgggtatg
     3661 gggtcggata cggcgcgggg agatttgttc tggagttctt ccggggagac gctcgcgtgg
     3721 gcgtttcctt tttctcagtt tcgcagctgc tcgcagcagg agtgttgatg ggcggcctgt
     3781 tgctgcttcg attcagaatt cagatagaat gcagggggaa atttcggagg aggtgagcgt
     3841 cttcttttct actctggata atccgtgaaa
//
LOCUS       DAOSWW010000135         3866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16433_length_3866_cov_3.554972, whole genome shotgun sequence.
ACCESSION   DAOSWW010000135 DAOSWW010000000
VERSION     DAOSWW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16433_length_3866_cov_3.554972"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PK442_03920"
     CDS             complement(<1..830)
                     /locus_tag="PK442_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86827.1"
                     /translation="MRKAGRILLLLLIAAIFIALSATAFMLAASTESASRVRLDWAHM
                     ANISAERVLSPADRSSPRAAMESFMNHMKEGYERLLKAKEFVSSAPGLLHQPPEALEE
                     AGRALYNFERAMHVLDLSQIPQVVRLQNARENILLLKEILDRVGIPRYDQIPGAQEVE
                     EQGLVRWTIPKTEIRLHRVESGIDEGRWLVTPASVAELGRSYRRVRHLPYLPGATENA
                     YEYYTASPGAFFPPKWAYFIPAWLTHRTYGQALWQWSILIISSILFILLLLAVRKLSF
                     "
     gene            1184..1930
                     /locus_tag="PK442_03925"
     CDS             1184..1930
                     /locus_tag="PK442_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOO86828.1"
                     /translation="MKKEEYRWFLKDTVRTTVDFSQTQQNLGYPPPPVQKPRPEGSHI
                     IPLPGPDEGGAPRDISVAEAIRRRSSHRKYSAKPLAVEELSFLLWATQGVRGDRDPVR
                     SYRTVPSAGCRHPFETYLAVFRVESLQKGIYRYLPFDHALLPLGCPSDLESRMSRAAH
                     NQPFAGKSAVTFIWTALPERTEWRYSEASVKVIALDAGHVCQNLYLACEAADAGTCAI
                     AAYDQELADSLIGVDGENEFVLYMAPVGKL"
     gene            2022..3491
                     /locus_tag="PK442_03930"
     CDS             2022..3491
                     /locus_tag="PK442_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO86829.1"
                     /translation="MLLKKNGVLLLVLAVCAAAVLGAATVFAEQSETVVFEVTADTPV
                     VQTGEARKVVVRALVRPYTRRETVRAPLAVALVLDKSGSMASDGKMDNAKRGALEALK
                     MLDSRDVATIIVYDDNASVLVRARSVGNGAKEPAFLRAISRVRSGGMTALHDGVVLGA
                     EQLEPFIDEGFIPRIVLLSDGIANVGPSSTEELARLGRGLARREMTITTIGLGLDYHE
                     DLMTALAAESGGNAYFARNARMLPEIFTRDMEDAVTLTARRVRISLRALGGARPARVI
                     GRPGNLTGDLLEASIDNLYSGEKYALFEMELPEGEEGAQLEAAEVVLEYIDPVTGDTV
                     RKSAPLTFSFTADGAEVEKRQRTDIVSQAALARNAEIREEAVRLADEGKAQEAAQMLG
                     DRTKQLRMLAPSVGTAAPQMDKESEYFEDLAESLLSTGGLSNVERKEVLNDAYMQKTQ
                     QAPVEVVSGDLPGDKPEDTPEDEAEEEEKEKEGEGNASK"
     gene            3588..>3866
                     /locus_tag="PK442_03935"
     CDS             3588..>3866
                     /locus_tag="PK442_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86830.1"
                     /translation="MEKMRDVLKRLTTPFSLTLLFSLAVLLPSVVLSMLALRAADREA
                     LHIERNLESALLSEVNLAAHRVADLLGSIATELMGEASEIPSNPAFLEQ"
BASE COUNT          823 a         1008 c         1229 g          806 t
ORIGIN      
        1 ttaaaactca gtttccggac ggccaaaagc aggaggatga acagaattga cgagataatg
       61 agaatgctcc actgccagag ggcttgaccg taagtacgat gcgtcagcca cgccggaatg
      121 aaataggccc acttgggggg aaagaaagct cccggagatg ctgtatagta ttcataagcg
      181 ttttcggttg ctcccggtag gtatggcaga tgccgtaccc ttcggtaaga acgtcccagc
      241 tcggcaacgg aggccggcgt gacgagccat cgcccctcgt cgatcccgga ttctacacgg
      301 tgcagacgta tttcagtctt gggtatggtc cagcggacga gtccctgttc ttcgacttcc
      361 tgggctccgg gtatctgatc gtaccggggg atgcccaccc ggtcgagaat ctccttcaag
      421 agaagaatgt tttccctggc gttctgaaga cggacgacct gggggatttg tgagaggtcg
      481 aggacgtgca tggcgcgttc aaagttatac agggcgcgtc ccgcttcttc cagcgcctcg
      541 ggcggttgat ggaggagtcc cggagcggag gagacgaact ccttcgcctt gagcagtcgt
      601 tcgtagcctt ctttcatatg gttcatgaag gattccattg ccgcgcgagg agacgatctg
      661 tctgcgggag agagcacgcg ctccgcgctg atgttcgcca tatgggccca gtcgagtcga
      721 accctggagg cgctctccgt cgaggcggca agcatgaagg ccgttgctga aagagcgatg
      781 aaaatcgccg cgatgagaag gagcagaaga atccggccgg cttttctcat gagttgaacc
      841 acttcctttc gtgagctgcg tatgcctgaa atgtggacga aatgaaaaaa gtcgtttgca
      901 caataaaggc tgaaacggcg ggtattctaa gagttttgcc gtgtgcacaa attgcagagt
      961 ttttgctgtg tgcaatacat tttatcagaa aatgcattct caagcaactt cggacggtgc
     1021 attcgtctgc gtcgatggat tcaggaggtt actgtttcgg ctttgccgct acagcgtcgc
     1081 agtatataat caaggagtga agtgaacgcc aagaactcaa gtgaacacca ggaactgcga
     1141 ccttcgcatg tattccgggt gttgccgggg agggatgatg agaatgaaaa aagaagagta
     1201 tcgctggttt ttgaaggata cagtacgcac gaccgtcgat ttttcgcaga cgcagcagaa
     1261 tcttgggtat ccgccgcctc cagtgcaaaa accgcgcccg gagggcagcc atatcattcc
     1321 gctgcccgga ccggatgagg ggggagctcc ccgtgatatt tccgtggcgg aggccatccg
     1381 tcgccgcagc tcccatagaa agtattcggc gaagcctctg gccgttgaag agttgagctt
     1441 tttgctgtgg gcgacgcagg gagtcagagg cgaccgcgat cccgtccgct cttaccggac
     1501 cgtaccctcc gcgggatgcc gtcatccgtt cgagacgtac ctcgctgttt ttcgggtgga
     1561 atcgctccag aagggaatct acaggtacct tccttttgac cacgcgctgc ttcccctcgg
     1621 atgtccgtcc gaccttgaat cgaggatgag cagggctgcg cacaatcagc cgttcgccgg
     1681 gaaaagcgca gtcacgttca tctggacggc gcttccggag cgtaccgaat ggcgctattc
     1741 cgaggcctcc gtgaaggtga tcgcgctcga cgcggggcat gtctgccaga acctctatct
     1801 tgcatgcgaa gccgcagacg ccggaacgtg cgccattgcc gcctacgacc aggaacttgc
     1861 cgacagtctc atcggtgttg acggagagaa cgagtttgtc ctctacatgg cgccggtagg
     1921 gaaactctga tgatttttac atttttgtga catccgcttg acggggcgat gacattctgc
     1981 cggttttttc gctgtatcct tcggaccagg aggtggtgaa gatgttgttg aaaaagaatg
     2041 gagtcctgtt gctggttttg gcggtgtgcg ccgcggcggt tctcggcgct gcaactgttt
     2101 ttgcggaaca gtctgagacg gtggtctttg aggttaccgc ggataccccc gtcgtgcaga
     2161 ccggggaggc tcggaaggtt gttgtcaggg cgcttgttcg tccatatacc agaagagaga
     2221 cggttcgggc cccgcttgct gtcgctctgg tgctcgacaa atcaggatcg atggcgtccg
     2281 acgggaagat ggacaatgcg aaacgtggag ctctcgaagc cctcaagatg cttgacagcc
     2341 gggatgtggc gacaataatc gtgtacgacg acaacgcatc cgttctggta cgggctcgct
     2401 ccgtcggaaa cggtgcgaaa gaaccggctt ttctcagggc gatttcccgg gttcgctccg
     2461 gaggaatgac agcgctgcac gacggcgtcg tccttggtgc ggagcagctc gaacccttca
     2521 tcgacgaggg gtttatcccc cgtatcgtgc tgctttccga cggaatcgcc aacgtaggtc
     2581 cctcgtcgac cgaggagctg gcgcgtctcg gcaggggatt ggcccgcagg gagatgacga
     2641 ttacgacgat agggctcggg cttgattacc acgaggatct tatgacggcg ctcgctgcgg
     2701 agagcggggg gaacgcctat tttgcccgca atgcgagaat gctgcccgag atcttcaccc
     2761 gggatatgga ggatgcggtg acgctgacgg cccggcgggt gcgcatttcg ctccgcgctc
     2821 tcggcggcgc gcgtccggcg cgtgtcattg gccggccggg aaacctcacc ggcgatcttt
     2881 tggaggcctc catcgataac ctctacagcg gagaaaaata cgcgctcttt gaaatggagc
     2941 tccctgaagg ggaagagggc gcccagcttg aagcggcaga agttgtcctt gagtacatcg
     3001 atcccgtgac cggggacacc gtgcggaaga gcgctccatt gaccttctcc tttaccgccg
     3061 acggcgccga ggtggagaag cgccagagga cggatatcgt ctcgcaggct gctctggccc
     3121 ggaacgcgga gatcagagag gaggccgttc gcctggccga cgaggggaaa gcgcaggagg
     3181 cggcgcagat gctgggagat cggacgaaac agcttcggat gcttgcccct tccgtcggga
     3241 cggcagctcc gcagatggac aaggaatcgg agtatttcga ggatctggcg gagagtctgc
     3301 tctcgacagg cgggctttcg aacgtggagc ggaaagaagt tcttaacgac gcctatatgc
     3361 agaagaccca gcaggctccg gtagaggtcg tgagcggcga tcttccaggg gacaagccgg
     3421 aggatacgcc ggaggatgaa gcagaggaag aagaaaaaga aaaagaggga gagggaaacg
     3481 cctccaaata aaaggcgatc cttccggaga acaggagata gtatgaagaa aaaggccggg
     3541 gacgttttcg tccctggcct tcacgtaaag ggaaggagga cggcgcaatg gagaagatga
     3601 gagacgtgct caaaaggttg actaccccgt tttctctgac gctgctcttt tccctcgccg
     3661 tactgctccc gagcgtcgtc ttgagcatgc tcgcgcttcg cgctgcggac agggaggcgt
     3721 tgcacatcga acgcaacctg gagagcgccc tgctctcgga ggttaatctc gccgctcacc
     3781 gggtggccga cttgcttggg tcgattgcaa cggaactgat gggggaggcg tcggagatcc
     3841 cttccaatcc cgcctttctg gagcag
//
LOCUS       DAOSWW010000136         3859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16477_length_3859_cov_3.266036, whole genome shotgun sequence.
ACCESSION   DAOSWW010000136 DAOSWW010000000
VERSION     DAOSWW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16477_length_3859_cov_3.266036"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PK442_03940"
     CDS             complement(<1..905)
                     /locus_tag="PK442_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOO86831.1"
                     /translation="MIFPNYWMLFAILFPMTAAFILQLLHHFAPSKTGGWILLSCGIT
                     SLLTFGAAVSAISGVRVLRLGGWSADLGILLVSDPLSAVFLFISAFGGAAAVWTTFTN
                     ISKSGPSAEQWRLCGLFFILWASLNGILTTGDLFNLFIFYEILAVAAYVLTGASKTRE
                     ALEAGLKYLVLGTVGALLILLATSMLYMGGGILNFALLSSAVGGIPHGALAVASGFMV
                     AGLGLKMGMVPFHFWLPDAHSSASTGVSMFLSGVVVKASFYSLLRVTTLLFPAEQAGF
                     SIPDTLLVLGLLSLFTGHISAQRQT"
     gene            complement(979..1314)
                     /locus_tag="PK442_03945"
     CDS             complement(979..1314)
                     /locus_tag="PK442_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HOO86832.1"
                     /translation="MAVMSFLTVSLLFALGLFTVLSQRNVFKTVIGLTLMESAVLLLL
                     ISWAYIPGGSAPILPLQGKGVDPLPHSFTLTAIVIGACDIALAFAFIMKLHRFERSVN
                     VDDFRRLKG"
     gene            complement(1302..2297)
                     /locus_tag="PK442_03950"
     CDS             complement(1302..2297)
                     /locus_tag="PK442_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOO86833.1"
                     /translation="MNEYLLAFLYFFILLTAVVAVFLDDLLSSFISLSVTGVLMATVF
                     ALLRAPDVALTQAIINSGLVTSLFLVAYSRTQRTPPPDGECRECPFWLRWPGAALVSA
                     LFAAVLFRGLFVVSGERILGRASAHYLSHTLEETGALNVVAAILLDYRAFDTLGETTV
                     IFTAVFGISLLLSGGRYVHSGHGLSFIVKRGMALLTPFILLYAASILFLGHLTPGGGF
                     QGGSVFATVAILICVVYGTSFEAARISPKTKETFEAGGAVLFVFIGLLGIAWGGGFLA
                     NLFGPFSAGTPGSLFSGGNMLLLNLAVGMKVGAGLSSIFYTMIKILELEDDSWPS"
     gene            complement(2294..2620)
                     /gene="mnhG"
                     /locus_tag="PK442_03955"
     CDS             complement(2294..2620)
                     /gene="mnhG"
                     /locus_tag="PK442_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOO86834.1"
                     /translation="MIPLLRFLGGISIFAGVFFSFAAVVAILRFPDVYTRLHGGTKAL
                     SGGAILILLGSMPYAGGVAAALKLFLIAVFFLVSNPMASHAIARACHRRTLANRPIDE
                     KKRDDV"
     gene            complement(2617..2874)
                     /locus_tag="PK442_03960"
     CDS             complement(2617..2874)
                     /locus_tag="PK442_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOO86835.1"
                     /translation="MILAALWGMVISGLIGFVRVVTGPSPADRLVAADALSVIFTMVL
                     VLLSLYIETPFLLDVALAFSMLSFADMLLMAKYFEHGELHK"
     gene            complement(2871..3410)
                     /locus_tag="PK442_03965"
     CDS             complement(2871..3410)
                     /locus_tag="PK442_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOO86836.1"
                     /translation="MKTFFRERMVLMPFRSGVSVLAVALFAVWMALSGSLSPAFLLVG
                     LLVAFLIARVSWRPFFGRHIRFSPKNFLRRTVAFVSFVPFFLFAMVKASWDVALQAFR
                     PELSISPGTFVYTPSPAFKGSIVALANAVTLTPGTLTVDILHGRRSLLVHALMAEGGE
                     ETLRNDVRRLEEGIAGFTE"
     gene            complement(3545..>3859)
                     /locus_tag="PK442_03970"
     CDS             complement(3545..>3859)
                     /locus_tag="PK442_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86837.1"
                     /translation="PVLLFGFFPGWIPRFLGVSEPELFSAAKCGESVLVFFIGALLFF
                     LLRTLLKPAEKNIPDADLLFAATGCAVRKAIVFVRRLQGGNLREYIFFSLLAILMLVL
                     LL"
BASE COUNT         1104 a         1073 c         1077 g          605 t
ORIGIN      
        1 ctcgtctgcc gctgcgccga aatatgcccg gtgaagagcg agagcagccc cagcacaaga
       61 agggtgtcgg gtattgaaaa acctgcctgt tcggccggaa agagcagcgt cgtcaccctg
      121 agcagagaat agaaagacgc cttgacgaca acaccggaga ggaacatgct tactccggtc
      181 gaagccgacg agtgcgcatc cggcaaccag aaatggaacg ggaccatgcc catcttgagc
      241 ccgagccccg caaccatgaa tccggacgca acagcaaggg ctccgtgagg aataccgccc
      301 acggctgaag agagcagcgc gaaattcagt attccgcccc ccatgtacag catgctcgtc
      361 gcgagaagaa tcagcagcgc gccgaccgtt cccagaacga ggtatttgag cccggcttca
      421 agggcctcgc gcgttttcga ggctcctgta aggacgtagg cggctacggc aagaatttca
      481 taaaaaataa agaggttgaa gagatcgccg gttgtaagaa tcccgttgag cgaagcccag
      541 aggatgaaaa aaagaccgca gagacgccac tgctccgctg acggccctga tttggagatg
      601 ttcgtaaaag tcgtccacac ggcagccgct cctccgaaag cggagatgaa aagaaaaacc
      661 gccgacagag gatccgaaac aaggagaatt cccaaatcgg cgctccatcc cccgagccgg
      721 agcaccctga cgccggaaat cgcggaaacg gccgcaccga acgtcagcaa cgaggtgata
      781 ccacaggaaa gaaggatcca tcctccggtc ttcgagggtg cgaaatgatg cagcagttga
      841 agaataaagg ccgccgtcat cgggaagagg atagcgaaca gcatccaata gtttggaaag
      901 atcatggccg caacaatctc cggtctgcat cgcagacagg gatgtcttca ggagagaaga
      961 agcgcaaggg ttctctcatc atcctttgag cctcctgaaa tcatccacgt tcacactgcg
     1021 ctcgaaacgg tgcagtttca tgataaaggc aaaggccaac gcgatatcgc acgcgccgat
     1081 gacgatcgcc gtcagggtaa acgaatgcgg aagcgggtcc accccttttc cctgcaacgg
     1141 cagaataggg gccgagccgc ccgggatata cgcccatgaa atcaagagca aaagcaccgc
     1201 cgattccata agcgtcaaac cgatcacggt cttgaaaacg tttctctggg aaaggacggt
     1261 gaagagcccc agcgcaaaga gcagcgagac ggtaagaaag ctcatgacgg ccatgagtcg
     1321 tcctccagtt cgagtatttt tatcatggtg tagaagatcg aggaaaggcc cgcccccacc
     1381 ttcataccta cggccaggtt gagcagcagc atgtttccgc cgctgaacag agaaccgggc
     1441 gtacctgcgg aaaacggacc gaaaagattg gccaggaagc ctcctcccca cgcaattccc
     1501 agaagtccga tgaagacgaa gagcaccgcg cctcccgctt cgaacgtctc tttggtcttt
     1561 ggggagatac gcgcagcctc gaagctcgtg ccgtagacaa cgcagatgag aatcgccact
     1621 gtggcgaaga cgctcccccc ctggaatcct cccccgggcg tcagatgacc gaggaagaga
     1681 atgcttgcgg catacagtaa aataaaagga gtcagaagcg ccattccgcg cttgacgatg
     1741 aaagagagcc catgcccgga gtgaacatat cttccgccgg acaaaagcag cgagataccg
     1801 aagaccgccg taaaaatgac tgtggtctct cccagcgtat cgaaggcccg gtagtcaaga
     1861 aggatggcgg cgacaacatt gagcgccccg gtctcttcaa gagtatgcga gagatagtga
     1921 gcggacgctc tccccaagat gcgttcgccc gacactacaa ataagccgcg gaagagcacg
     1981 gcggcgaaga gagcgctgac aagggcggct cccggccagc gaagccagaa aggacactcc
     2041 cggcactctc cgtcgggagg aggcgtacgt tgcgtccggc tgtatgccac aagaaacagc
     2101 gatgtaacaa gtcccgagtt gataatggcc tgcgtcagcg caacgtcggg cgcacgcaaa
     2161 agagcgaaga ccgtcgccat aagaaccccc gtcacagaga gggagatgaa cgagctgaga
     2221 aggtcgtcga gaaatacggc aacaaccgct gttaacagaa taaagaaata gagaaaggcc
     2281 agaagatact cgttcatacg tcgtccctct tcttttcatc gatcggcctg ttcgcaaggg
     2341 ttcttctgtg gcatgccctt gcgatggcgt gcgacgccat cgggttcgaa accaggaaga
     2401 agactgcgat gaggaagagt ttcagcgcgg ccgcaacgcc tccggcatac ggcatggagc
     2461 caaggagtat caggatcgcc cctcccgaaa gagctttcgt tcctccgtgc aggcgggtat
     2521 acacatcagg aaagcggaga atggccacca cggcggcgaa gctgaaaaaa acgcccgcaa
     2581 agatgctgat tcctccgaga aaccgaagaa gaggaatcat ttgtgcagtt ctccgtgttc
     2641 aaaatacttc gccatcagca gcatatcggc aaaggagagc atcgagaagg caagcgctac
     2701 gtccaaaaga aaaggggtct cgatatacag gctaagaagc acgagcacca tggtaaagat
     2761 caccgagagc gcatccgcag cgacaagtct gtccgcaggg gacggcccgg tcactacccg
     2821 tacaaaccct atcaaacccg atatcaccat accccacaga gcggccagaa tcattcggta
     2881 aaccccgcta ttccctcctc cagccgccgg acgtcgttgc gaagagtctc ctctccccct
     2941 tccgccatga gggcatggac gagcagagaa cgcctgccgt gcaggatatc gacggtaagc
     3001 gttcccgggg tcagggtcac ggcgtttgcg agagcaacta tggagccttt gaaagccgga
     3061 gaaggcgtat atacaaatgt gccgggggat atcgagagct ccgggcgaaa cgcctggagg
     3121 gcgacatccc atgaggcttt gaccattgca aaaagaaaga acgggacaaa ggagacaaaa
     3181 gcgacggtcc gccgaaggaa gtttttcgga gagaagcgga tatgccgccc gaaaaacggt
     3241 ctccaggaga cacgcgcgat aagaaaggct acgagcagtc caacgagcag aaaagccggg
     3301 gatagcgatc cgctaagagc catccacacc gcaaagagcg caacagcaag aacggaaaca
     3361 ccggagcgaa agggcatcag caccatcctt tctctgaaaa aagttttcat aataatacca
     3421 tccttctctt gctacgcgag caacaaagta aagaagaaat ccgcatcgaa agctttccga
     3481 ggtcatggag agatcgccgg atcaaccgac cgggcggcat tcggaaagtc cgccccgcga
     3541 acggtcatag caacaggacg agcatcagaa tggcaagcag cgaaaagaag atatactctc
     3601 tgagatttcc tccctgcaga cgccgcacaa acacaatggc ttttctgact gcgcatcccg
     3661 tcgctgcgaa gagaagatcc gcatcgggaa tattcttctc ggcaggtttc aggagagttc
     3721 tgagcaaaaa gaaaagaagc gctccgatga aaaacacgag cacgctctct ccgcacttcg
     3781 ccgcagaaaa aagctccggc tccgatacgc cgagaaagcg aggaatccac cccggaaaaa
     3841 aaccgaaaag gagtaccgg
//
LOCUS       DAOSWW010000137         3830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16671_length_3830_cov_3.140397, whole genome shotgun sequence.
ACCESSION   DAOSWW010000137 DAOSWW010000000
VERSION     DAOSWW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16671_length_3830_cov_3.140397"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1405)
                     /locus_tag="PK442_03975"
     CDS             complement(182..1405)
                     /locus_tag="PK442_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO86838.1"
                     /translation="MSDWDFFHLCGSGAPADIRAALEEGANADISVKEYGWSALMNAA
                     RYNSHPEVVTILVKAGADVHVIDANGWTALMLALRYNPNPEVTKALLRAGGDVWAKTV
                     NVKNSEGWTAMMYAACYKDDETVVALYKAGADARAQDAEGVSVLMRAAAVNPDPVAVR
                     AVVDAVPGTGGFDSLGRSPLMWAAARNPNPEVTRALLSAGANVYARDKENASVLMHAA
                     AYNPNPDVCLEILKAKTNVHLRDKYGRNALMHAARSNPNPEVATLLPHAGVDPAAKDG
                     AGRTALMWAARENPNPAMIAALVRFGAAVGVRDKNGVDALMFAAGYNENPEMTMAVMH
                     AGAAVNAKDANGTTPLMYAAKYSLHPQVLDVLLNAGANPKLRDKQGKRALDYASFNGQ
                     IKGTEAHRRLYQVSR"
     gene            complement(1733..1808)
                     /locus_tag="PK442_03980"
     tRNA            complement(1733..1808)
                     /locus_tag="PK442_03980"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1773..1775),aa:Thr,seq:ggt)
     gene            complement(1824..1908)
                     /locus_tag="PK442_03985"
     tRNA            complement(1824..1908)
                     /locus_tag="PK442_03985"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1872..1874),aa:Tyr,seq:gta)
     gene            complement(1924..1999)
                     /locus_tag="PK442_03990"
     tRNA            complement(1924..1999)
                     /locus_tag="PK442_03990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1964..1966),aa:Thr,seq:tgt)
     gene            complement(2037..3002)
                     /locus_tag="PK442_03995"
     CDS             complement(2037..3002)
                     /locus_tag="PK442_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOO86839.1"
                     /translation="MIQHIARGDIRKTLLYPLLSTGVALLFIVLLPVLQRGLSPEISR
                     FLLRGAKIFLVASVAWLSIGAVFLLEIVFHKKYDVSVADNLNARKFQTQFVIFKRLLL
                     TMIVFVAFSIILLSIDDFRRIGTSLLASVGVVGIVVGLAAQKTVATILAGIQLAVSQP
                     IRIDDVVVAEGEWGRVEQITFTYVVLRLWDQRRLVLPTTYFLERPFQNWTRISADLIA
                     TAYFRVDFSAEVEKLRERFFSILGSTPLWDGKARALQVTDCGERSLEIRALMSAANGS
                     SAWDLRCLVREEMTAWLRDEHPEWLPKVRLLPVEEEKNLFSPSNG"
     gene            complement(3063..3362)
                     /locus_tag="PK442_04000"
     CDS             complement(3063..3362)
                     /locus_tag="PK442_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86840.1"
                     /translation="MKMVWLHCSETLAQETTEILDAAGIEYYSVWRNVLGKDNRGNRT
                     RWDDAVFPGKNWALQFLCTDEAIEELKRRFDELSGDPYVKESGIAMYVAEAERAL"
     gene            complement(3359..>3830)
                     /locus_tag="PK442_04005"
     CDS             complement(3359..>3830)
                     /locus_tag="PK442_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO86841.1"
                     /translation="GQYEAYLDPFVIMFSIPFALTGVVFAFLLTGLYISLQAMLGVVM
                     LVGIVVNNAIVLVDYVNLLRARGTPLRRSLIEAGGRRLRPVLMTTLTTFFGMLPMAVS
                     NAIGSEVWKPLAVSVMGGLVVSTLITLLLVPVMYSFFEEKIRRKGRFAEAVNER"
BASE COUNT          839 a         1176 c         1008 g          807 t
ORIGIN      
        1 ttgccgccgt caaggcagaa cccggtagcg ctcgccctgg acacactctc agcgtgtgga
       61 ttataccaaa gaaaacgaaa aaaggaatac aaaaaaatct ttcgtcgcga tgttgcaact
      121 gttccacaag acatccggaa aaatgccgag ctgccctctc atgcaagaac aacctggcga
      181 gtcaacggga aacctggtac agccgacgat gcgcctcagt gcctttgatc tgcccgttga
      241 acgaagcata gtcaagcgct cgttttccct gcttatcccg aagtttcgga ttggctccgg
      301 cgttcagcag aacatcgagc acctgcgggt gcagcgaata cttcgccgcg tacatcagcg
      361 gcgtcgttcc gttcgcatct ttggcattga cagcggcgcc cgcatgcatt acggccatgg
      421 tcatttcggg gttctcgttg taccccgccg caaacatcag tgcgtccacg ccatttttat
      481 ccctcacacc gacggcggcg ccaaatcgta ccagcgcggc aatcatggcc ggattcggat
      541 tctctcttgc cgcccacatc agcgcggttc ttccggcccc gtccttcgcg gcagggtcta
      601 ccccggcgtg cggaagaagc gtcgcaactt caggatttgg attgctccgg gctgcgtgca
      661 tcaacgcatt cctcccgtat ttatccctca agtgtacgtt cgttttcgct ttgagaatct
      721 ccagacacac atccggattc ggattgtacg cggccgcgtg catcagtacg cttgcgttct
      781 ccttgtctct ggcatacacg ttcgctccgg cgcttaacag cgcgcgggtc acttcgggat
      841 tcggattccg ggctgccgcc cacataagcg gacttctccc aagactgtca aatcctcctg
      901 taccgggcac ggcgtcgaca accgccctca ccgcaacagg atccgggttg acggcggcgg
      961 cgcgcatgag cacgctcacc ccttccgcat cctgagcgcg ggcatcggcg cccgccttgt
     1021 acagagcgac gacggtctca tcgtctttgt agcaggcggc gtacatcatt gcggtccagc
     1081 cttcgctgtt cttgacgttc accgttttcg cccatacatc tcccccggct cgcagaagcg
     1141 ctttcgttac ttcaggattc ggattgtagc gcagtgccag cataagcgcg gtccagccgt
     1201 tcgcgtcgat aacatggaca tcggcgccgg ccttcacgag aatcgtcact acttcggggt
     1261 gcgagttgta gcgcgcagcg ttcatcaacg cgctccatcc atactccttg acgcttatat
     1321 ccgcattcgc gccctcttcg agcgccgctc ggatatctgc cggagcgccg ctcccgcaga
     1381 ggtggaaaaa gtcccagtcg ctcatggtgg caaacgccgc attggttacc aatagcacga
     1441 atgctgcaac tagcgaaaag cacgcgcttc tccgacgcat acaatcacat cccctgaata
     1501 gaatttgcga tatcatgctg aattgtaaca caaaagcatc tcaacataaa tttttttctt
     1561 caaacagggg agatttcttc gtcggagaat cgcgcgcgtg gcttccagca attccttaag
     1621 gcgattctac tcttctttac cggaggggtc tttggaaatg gatgccttgg cgcacatccg
     1681 gaccaacaaa aacagggctt cagcatgtag ccgaagccct gaatctcttc actggagccg
     1741 acttccggac ttgaaccgga gaccccatcc ttaccatgga tgtgctctac ctgctgagct
     1801 aagtcggcat tgatctaaaa aagtggtggt gggagaagga ttcgaacctt cgtagacggt
     1861 gtcggcagat ttacagtctg ctgcctttga ccactcggcc atcccaccac gtaattgtca
     1921 agctggagcc ggcacccgga cttgaaccgg ggacctgctg attacaagtc agctgctcta
     1981 ccaactgagc tacaccggct caacgaaggt atattatagc gggtgaaacg aagatgtcaa
     2041 ccgttggacg gcgaaaaaag atttttttcc tcttccacgg gcaacagtcg cactttaggc
     2101 agccactccg gatgttcatc gcggagccat gccgtcattt cttcacggac aaggcagcga
     2161 agatcccatg cggaagagcc gttggccgcg ctcatcaacg cgcggatttc cagcgagcgc
     2221 tccccgcagt cggtcacctg aagcgccctg gccttgccgt cccacagcgg agtcgatccg
     2281 agtatcgaga aaaatcgctc gcggagtttc tcgacttccg ccgaaaaatc gacgcggaag
     2341 taggcggtgg cgataagatc cgccgagatg cgcgtccagt tctggaatgg acgctccaaa
     2401 aagtaggttg tgggcaacac aagccgccgt tgatcccaca gcctcaggac aacgtaggtg
     2461 aaggtgatct gctcgacgcg cccccactcc ccctccgcga cgacgacgtc gtcgattcgg
     2521 atcggctgcg acaccgcgag ctgaattcct gcaagaatag tggcgaccgt tttctgcgcc
     2581 gccaagccga cgacgattcc gacgacgcct accgacgcca gcagcgacgt cccgatgcgc
     2641 ctgaagtcgt cgatgctgag caggatgatg gaaaacgcga cgaagacgat catcgtcagc
     2701 agaagccgct taaagatgac gaactgcgtc tggaactttc gcgcattgag attgtcggcg
     2761 acggaaacat cgtacttctt gtgaaagaca atctccagca aaaacacggc tcctatggag
     2821 agccatgcga ccgatgcaac aagaaatatc ttcgcgccgc gcagcaaaaa acgagagatt
     2881 tcgggcgaga gtcccctctg gagcaccggg agcaatacga tgaagagcag cgcgacccct
     2941 gtggaaagca gcgggtacag aagcgtcttc cgaatgtcgc ccctggcgat gtgctgtatc
     3001 atgagcgtcc ctcctttcgg aacttccttt cattctacac caaccgtcgc ccctcccccc
     3061 atttaaagcg ctctctccgc ttcggcgaca tacatggcga tcccggactc tttgacgtac
     3121 ggatcgccgc tcagttcatc gaatcgcctc ttcaactcct cgatggcctc gtcggtgcag
     3181 agaaactgca gcgcccagtt ctttcccgga aagacggcgt cgtcccaacg tgtcctgttt
     3241 ccacggttgt ccttccccag aacgttcctc catacgctgt aatactctat gccggccgcg
     3301 tcgagtattt cggtagtctc ctgggcgagc gtttcgctgc agtgcagcca taccatcttc
     3361 atcgttcgtt caccgcctcc gcgaagcgtc cttttcttcg tattttttcc tcgaagaagc
     3421 tgtacatgac cggaaccaac agcagtgtga tcagggttga gacgacaagc ccccccatga
     3481 ccgagacggc gagcggtttc cagacctcgg agccgatcgc gttgctcacg gccatcggca
     3541 acatgccgaa gaaggtggtg agcgtggtca tgagaacagg ccgaagacgc cgtccccccg
     3601 cttctatcag cgaccttcgg aggggggttc cccgcgctcg cagcagattc acgtagtcca
     3661 cgaggacgat ggcgttgttc accacgatgc ccacgagcat cacgacgccc agcatcgcct
     3721 ggagagagat gtagagcccc gtaagcagaa aggcgaagac cacgccggta agggcaaacg
     3781 ggatgctgaa catgatgacg aacggatcga gataggcttc gtactgcccc
//
LOCUS       DAOSWW010000138         3828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16679_length_3828_cov_4.103101, whole genome shotgun sequence.
ACCESSION   DAOSWW010000138 DAOSWW010000000
VERSION     DAOSWW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16679_length_3828_cov_4.103101"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..868
                     /gene="cdaA"
                     /locus_tag="PK442_04010"
     CDS             56..868
                     /gene="cdaA"
                     /locus_tag="PK442_04010"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOO86842.1"
                     /translation="MFDTLRWQDFLDILVIALIVHRLLLLIVGTRAMQLVKGLLILGV
                     VAAAARLLDLRTLSWFLGQTLGILLIAIPIVFQPELRKMLEELGRGGGLWRRKRAAEK
                     AEAFSHEVSRAIGYLKGQKIGALIVLERNTGLKDFWRTAIKLNSEISQELLITIFWEG
                     TPLHDGAVILDRERIIAASCYLPLTENSDLSRWIGTRHRAALGVTEVSDAIALVVSEE
                     RGEVSLAINGRLSKNLKEAQVHKLLSHYFSGEEAEHKPLMERLQEDLRALWS"
     gene            894..2120
                     /locus_tag="PK442_04015"
     CDS             894..2120
                     /locus_tag="PK442_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOO86843.1"
                     /translation="MSESGKIDAILASPLFLKIMAGVIALALWFYVSGNRSTETTRTL
                     VVPLEYLNVSPQTTLKTAVQEVEIVLSGAQYVLSAIPQDGVVCEVDLRGVGVGKYRIP
                     VRSIVPKDVKIMDVKPTHVDVELARFIDRLIPVEIAVDKGLPPGLFLDMVEILPKNVS
                     VKGSERDIAKIDAVKIVPTLDELKAGGEHTLAVEVVKSEAFEDEVVVEPKTVKLSAVL
                     AEGVPKKSVPVNARIIGEPGGDFKLKAVVVEPAVVTVEGPLAKLNAVTRVDTETIDLT
                     GISKEQNIVIPLRAPEDQMVTIVGAASVRINVLLTPFTVTRLLSRLNVEVDGRSVYPG
                     WKVEPNIVNVTVEGVPSEVNALDEKDVVIRPFVNVTNVVSRRLTVPVQIQNKSGGKLK
                     VVKIDPASVTVIADIR"
     gene            2152..3522
                     /gene="glmM"
                     /locus_tag="PK442_04020"
     CDS             2152..3522
                     /gene="glmM"
                     /locus_tag="PK442_04020"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOO86844.1"
                     /translation="METGEKKVRCLFGTDGVRDVANRGVMTPEMSLRLGRSYILFLTE
                     RGIPRPRIVVGRDTRRSGHMLEAALSAGMASAGAEVFLLGVLPTPGVSFAVKKMSVHG
                     GAVISASHNPAEYNGIKFLDSSGKKLSDDVEGAIEEYMGDNLTDDWRPTGASVGDIHR
                     ATHLLDEYSEWIASLWGEETPFPSAGVIDCAHGAAVETAEKVFRRLGGEWRFVGNRPD
                     GLNINEGVGVMHMAHIAEAVRTNALPIGIAYDGDADRALLTDCRGRVLNGDIILWVLA
                     RWLAERNMLGSGVVATVMSNMALEDHLRKENIPVFRCPVGDRYVMERMSKEGSLLGGE
                     QSGHIILSSFVNTGDGLCTGLFFLKACLELGEDISTLADRFAPYPQILRNVEVSRDGK
                     VSGDFLRDLSSEAETLLQGAGRILIRPSGTEPLMRILVEARDEKLMEHVSEKLVNAIK
                     EQCRYQ"
     gene            3548..>3828
                     /locus_tag="PK442_04025"
     CDS             3548..>3828
                     /locus_tag="PK442_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOO86845.1"
                     /translation="MTSSISKCLFPVAGLGTRFLPATKEIAKEMLPLVDRPIIEYGVE
                     EALAAGCKEMVMITGRAKKAIEDYFDRSFELEELLKSRNKQTLYDEVISL"
BASE COUNT          828 a         1049 c         1161 g          790 t
ORIGIN      
        1 cctgtccgca ggggcgcgtt ccctgatact tctccgcccc ggagggcgat tgtatatgtt
       61 tgatactctg agatggcagg acttcctgga tatactggtc atcgccctga ttgtgcacag
      121 attgcttctg ctgatcgtgg gcactcgtgc gatgcagctc gtcaaggggc ttctcatcct
      181 cggcgtcgtt gctgcggcgg caagattgct cgacctgcgg acgctctcat ggtttttggg
      241 gcagacgctg ggtatcctcc tgatcgccat tccgatagtc tttcagccgg agctgcgaaa
      301 aatgctcgaa gagcttggca gggggggcgg cctctggagg cggaaacgag ctgctgaaaa
      361 ggccgaggcc ttcagccatg aagtatccag ggcgatcggt tatctcaaag ggcagaagat
      421 cggcgcgctg atcgtccttg aacgcaacac cgggctcaag gacttctgga gaactgccat
      481 taagctgaat tcggagataa gccaggagct tctcatcacc atcttctggg agggaacgcc
      541 tttgcacgac ggggcggtga tcctcgaccg cgagaggatc atcgctgcaa gctgctattt
      601 gccgctcacg gaaaactccg atctgtcgcg atggatcggc acccgtcaca gggcggcgct
      661 cggcgtgacg gaagtttccg acgcgatcgc ccttgtcgtc tccgaagaac ggggagaagt
      721 ttcgcttgca atcaacggcc gcctttccaa aaacctgaag gaggcccagg tgcataaact
      781 gctctcccac tatttcagcg gtgaagaagc tgaacataaa ccactcatgg aacgcctgca
      841 ggaagatctg cgggcgctgt ggtcatgaac gggaagaagg tgcgcaggcg cttatgtcgg
      901 aatccggaaa gatagacgcg atcctggcgt cgccgctgtt cctgaaaata atggccgggg
      961 tgatcgcgct cgctctctgg ttttacgtct ccggcaacag atcgaccgag acgacgagga
     1021 cattggttgt tcctctcgag tatctcaacg tctcgccgca gacaacgctc aaaaccgctg
     1081 tgcaggaagt ggagatagtc ctctcgggcg cgcagtatgt tctctccgcc attcctcagg
     1141 acggcgtggt ctgcgaggtc gacctcaggg gagtgggcgt cggaaaatac cggatacctg
     1201 tccgttccat cgttcccaag gacgtcaaga taatggacgt caaacctacg cacgtcgacg
     1261 tggaactcgc tcgcttcatc gaccgcctca ttccggtgga aatcgccgtc gacaaaggcc
     1321 ttcctccggg gctttttctc gatatggtcg agatacttcc taaaaacgtc agcgtcaagg
     1381 ggagcgaacg cgatatcgcg aagatagacg ccgtcaagat agttcccacc ctcgatgagc
     1441 tgaaggcggg aggggagcat acgcttgccg tcgaggtcgt caagtcggag gcgtttgaag
     1501 atgaggttgt tgtggagccg aaaaccgtca agctcagcgc ggtgctcgcc gagggagttc
     1561 cgaagaagag cgtcccggtg aacgcccgga ttatcggcga accgggcggc gacttcaaac
     1621 tgaaggccgt tgtcgttgaa cctgccgtcg tcacagtcga ggggcctctg gcgaaactca
     1681 acgccgtgac gcgggttgat accgaaacga tcgacctgac ggggatctcc aaggagcaga
     1741 acatcgtcat cccgttgcgc gctccggagg atcaaatggt gaccattgtc ggcgccgctt
     1801 cggtgcggat caacgttctt ctgaccccct tcaccgtcac ccgcctcctc tcccggctga
     1861 acgtcgaggt cgatgggcga agcgtctacc ccgggtggaa agtggagccg aacattgtga
     1921 acgtcactgt ggagggagtc ccctccgaag tgaacgcact cgacgaaaaa gacgtggtga
     1981 tacgcccctt tgtcaacgtc accaacgtcg tttccagacg cttgaccgtt cctgtgcaga
     2041 ttcagaacaa gtcgggcggc aaattgaagg tagtaaaaat tgatcctgcg agcgtcactg
     2101 taattgcgga tatcaggtaa tctcttatac gggcggggat ggtgaggctg catggaaact
     2161 ggtgagaaaa aagtacgttg tcttttcggg accgatggtg tgcgggacgt ggccaatagg
     2221 ggagtgatga ctcccgagat gtcattgcgg ctcggtcgct cctacattct tttcctgacg
     2281 gagaggggca tccctcgtcc aaggatcgtc gtcggaaggg acaccaggcg ttccgggcac
     2341 atgctggaag ccgctctttc cgccggcatg gcttcggccg gcgcggaggt ctttctcctc
     2401 ggagtgttgc ctactccggg cgtcagtttc gccgtaaaga agatgtcggt tcacggcgga
     2461 gcggtgatca gcgcctccca caaccctgcg gagtacaacg gaataaaatt cctcgattca
     2521 tccgggaaaa aactttccga cgacgtcgag ggggcgatcg aagagtacat gggggataac
     2581 ctcaccgacg attggcgccc caccggcgct tccgtgggag atattcatag agccacgcat
     2641 ctgcttgatg aatattccga gtggattgcc tcgctctggg gagaagagac ccccttcccg
     2701 tcggcggggg ttatcgactg cgcgcacggc gcggcggtgg aaacggctga aaaagtcttc
     2761 cgcaggctcg gcggcgagtg gcgctttgtt ggaaaccggc ccgacggact gaatatcaac
     2821 gaaggcgtcg gcgttatgca catggcgcac atagctgaag cggtgagaac gaacgcgctc
     2881 ccgataggca tcgcctacga cggagacgcc gaccgcgcgc tgctgacgga ttgcaggggg
     2941 agagtgctca acggcgatat catcctctgg gtactcgcgc gctggctcgc cgagcgcaat
     3001 atgctcggct cgggagtcgt tgccacggta atgagcaaca tggcgctcga agatcatctc
     3061 cggaaagaaa atatccctgt ttttcgctgc cctgtgggcg accgatacgt tatggagagg
     3121 atgtccaagg agggctcgct gctcggaggg gaacagtcag gccacatcat tctttcctcg
     3181 tttgtgaaca caggcgacgg actctgtacc ggccttttct tcctcaaggc gtgcctggaa
     3241 ctcggtgaag atatttcgac cctcgcagac cgtttcgccc cgtatccgca gattctgcgc
     3301 aacgtcgaag tttcccgcga cgggaaggtg agcggcgact tcctgaggga cctctcctcc
     3361 gaagcggaga cgctgcttca gggcgccgga agaattctca tccgcccctc gggcacggag
     3421 cctctgatgc gaatccttgt tgaagcgcgg gatgaaaagc tcatggagca cgtttccgag
     3481 aagctcgtga acgcaattaa agagcagtgc cgctatcaat aacgatcgtg tgaaaggtgg
     3541 ttgccgtatg acttcttcga tatcaaaatg cctcttcccc gtggcgggac tgggaacgcg
     3601 tttcctcccc gcgacaaaag agatcgcgaa agaaatgctg ccgctcgtcg acagacccat
     3661 cattgaatac ggcgttgaag aagcgcttgc cgcgggatgc aaagagatgg tcatgatcac
     3721 cggccgggca aagaaggcaa tcgaagacta cttcgaccgc tcttttgaac ttgaagagct
     3781 gcttaagtca cggaacaagc agaccctcta cgacgaggta atctcgct
//
LOCUS       DAOSWW010000139         3817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16758_length_3817_cov_3.605529, whole genome shotgun sequence.
ACCESSION   DAOSWW010000139 DAOSWW010000000
VERSION     DAOSWW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16758_length_3817_cov_3.605529"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PK442_04030"
     CDS             complement(<1..433)
                     /locus_tag="PK442_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86846.1"
                     /translation="MKFVDFTRISVRGGRGGNGCLSFRREKFIPKGGPDGADGGRGGD
                     IILEAAPGALTLADFEYEKKFQAGNGEPGKGGMKSGGAGNDKIVIVPCGTIVYDDDTG
                     QMLTASLMDYALPQFGVIDGFTTLLDQSVPTARNPLGAKGVG"
     gene            complement(593..880)
                     /gene="rpmA"
                     /locus_tag="PK442_04035"
     CDS             complement(593..880)
                     /gene="rpmA"
                     /locus_tag="PK442_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOO86847.1"
                     /translation="MKWNFDLQLFAHKKGQGSSTNGRDSEGKRLGIKIYAGQTVQAGN
                     ILVRQRGTKIHPGKNVGLGKDYTIFATSAGTVRYETKGSRKIVSVVLQAAE"
     gene            complement(864..1205)
                     /locus_tag="PK442_04040"
     CDS             complement(864..1205)
                     /locus_tag="PK442_04040"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOO86848.1"
                     /translation="MTEISVSFDEERPVALVAEGHSGYAERGEDIVCAGVSALLQALL
                     FGFEKVVSSPGFSFSINEDSARMVLDWRRSPPEQTAVLVETIVGSLKEIARVYPEHVR
                     ILEVPVDEMEF"
     gene            complement(1208..1525)
                     /gene="rplU"
                     /locus_tag="PK442_04045"
     CDS             complement(1208..1525)
                     /gene="rplU"
                     /locus_tag="PK442_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOO86849.1"
                     /translation="MYAIVETGGKQYRVQEGDLLKVESLGLEEGASVVFDKVLLLGRD
                     DAVVVGAPYVKGATVTASVTGNGREPKILVFKFKSKKNYRRMRGHRQNYSEIRIDTLT
                     AGV"
     gene            complement(1634..3364)
                     /locus_tag="PK442_04050"
     CDS             complement(1634..3364)
                     /locus_tag="PK442_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO86850.1"
                     /translation="MIEEISIKNVGGIASISMRLETGFTVITGESGAGKSSLVRALEL
                     IGGKRSQALFIRSGEEEASVEAVFSGSAERVSKTLQPRDEDGLLFARRILSRGGRNRC
                     FFQDKATPFSTFASLMNDRMRIQSQFAQIELLDPKRQMDILDFCCGAQAEHLRAALEN
                     TFDEALRCDRELRAVKSKEQELKLRFRDAEAIVEALRPLQISPGCDLLWERELDEKNR
                     TFQQARKVRENLLELTGGASNDGMIDRLESCALELIRRLFLEKTDVEASFNEGLSFLH
                     SFISSIEEKASALSPEKIERERDLIEKKLGILRKIKRTTGTRTAEELVSWTSDAQEAI
                     GWLREEGRLSSELLLCAKKLRREASSLAMELRALRQRAADSLEKEVNRHLQELGMAES
                     VFSVKLLPLDKIRSSGADEISFTLSSGGSEEMPVNKSASGGELSRILLALQLALPEET
                     LPPTLIFDEVEAGLGGKAALLAGYKLLALSRKTQVILVTHEATIASLADHHFLVKKTT
                     DCVEVKKLERDDRIGEVARMLSGDASLQEAREHAERLLYSAESTECSQSGNISVLAGE
                     GDLAALRGKV"
     gene            complement(3370..>3817)
                     /locus_tag="PK442_04055"
     CDS             complement(3370..>3817)
                     /locus_tag="PK442_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOO86851.1"
                     /translation="LAKGALARVMHIEVHIGDKLLNTLPADGIIVSTPTGSTAYALSA
                     GGPIIPPHVPCMLVVPICAHTLYSRPVVAGEDDTVTLVPRGNHRDLLLTQDGQLGYEI
                     LPGDRIEISLSRTQKIDVLLLPSRNYFDLLQEKLRWGQGIVTFEKE"
BASE COUNT          853 a         1104 c          919 g          941 t
ORIGIN      
        1 cgccaacccc cttggcaccg agcggattgc gcgcggtggg cacggactgg tcgagcaggg
       61 tcgtgaagcc atcgatcacg ccgaactgcg gcagcgcata gtccatcagg ctcgcggtca
      121 gcatctggcc ggtatcgtcg tcatagacaa tggtaccgca gggaacgatg acaattttgt
      181 cgttccccgc tccgcctgat ttcataccgc ccttgcccgg ttctccgttt ccggcctgga
      241 atttcttttc atattcaaaa tcggcaagcg tcaacgctcc cggagccgcc tccagaatga
      301 tgtcgccccc tcttcctccg tcggcgccgt caggaccgcc tttcgggata aacttttccc
      361 tacggaagct aagacacccg ttccccccgc gacctccgcg gacagatatt cgtgtaaaat
      421 caacaaattt catgtgatca ccggccgaaa ggccttttcc tttatcattt caaaaaatca
      481 agggccttga tgatccccta agggcgagag ccgacgtaaa aagcagacgg tctgttctta
      541 aggtgatgcg aaggcccttg ttgaatgcgt cgtgttcaaa aagtccggag aattactctg
      601 ccgcttgcag aactactgaa actattttcc tgctcccttt tgtctcgtaa cgcacggttc
      661 cagcgcttgt cgcaaaaatc gtatagtcct taccaagacc gacgttcttg ccaggatgga
      721 tttttgttcc tctttggcga accaaaatgt tgcccgcctg taccgtctgg ccggcatata
      781 tctttatccc gagcctcttt ccttcgctgt ctcttccatt cgtactgctt ccctgccctt
      841 tcttgtgggc aaagagctgc aggtcaaaat tccatttcat caacaggcac ctccagaata
      901 cggacatgct ccgggtatac ccgggctatt tccttcaagg atcccactat agtttccacg
      961 agaaccgccg tctgttccgg aggggatcgt ctccagtcca ataccattct cgcgctgtcc
     1021 tcattgatgc tgaaagaaaa accgggagaa gaaacaacct tctcaaaacc gaaaagaagc
     1081 gcctgtaaaa gtgcggagac gcccgcacat acaatatctt cgccccgttc ggcatacccc
     1141 gaatgacctt ctgcgacaag agcgacaggg cgctcctcgt cgaaggaaac ggagatctcg
     1201 gtcaaaatca aactccagca gtcagagtat cgattcgtat ctcagagtaa ttctgtctgt
     1261 gccctctcat acgccgatag tttttcttgc tcttgaactt aaaaacgagg atcttcggtt
     1321 ctctgccatt ccccgtcacc gaagcggtca ccgtagctcc cttgacatag ggggcgccga
     1381 ctacaacagc gtcatccctt cctagaagaa ggaccttatc aaaaaccacc gaggcgcctt
     1441 cttcaagacc gagtgattcg actttcaaaa gatcgccctc ctgaacacgg tactgtttgc
     1501 ctcctgtttc aacgatcgcg tacattgttt tcctcctccc gccgggaaga ccaggcgctg
     1561 ctcagcagct tcaacctgtc ccgtgcgatt taaacatcca accgtagtat tatacggagc
     1621 gaaaaggtta aagtcaaact tttcctcgca gggcagcgag atcaccctct ccggcaagga
     1681 cgcttatatt gcctgattgg ctgcactccg tactctccgc ggagtagaga agccgttcgg
     1741 catgttctcg agcctcctgc aaagaggcgt ctcctgagag catccgtgct acttctccga
     1801 tgcggtcgtc tctttccagc tttttgactt ctacacaatc cgtcgttttc ttgacgagaa
     1861 agtgatgatc cgcgagcgat gcaatcgttg cctcgtgcgt tacaaggatc acctgtgttt
     1921 ttctggagag agcgagcagc ttgtatccgg ccagaagcgc agcctttccg ccaagcccgg
     1981 cctccacttc atcgaagata agagtcgggg ggagcgtttc ctcaggcaac gcaagctgga
     2041 gcgcgagcaa tattctactc agttcgcctc cggaagcaga tttattgaca ggcatttctt
     2101 cagagcctcc cgaggagaga gtaaatgaaa tttcgtcagc cccgctgctg cgtattttgt
     2161 ccaacggcaa aagtttcacc gaaaaaacgc tctcggccat gccgagctcc tgaaggtgcc
     2221 tattcacttc tttttccagt gaatccgctg cccgttgccg gagtgcgcgc agctccatcg
     2281 ccaatgagct tgcctcgcgg cgtaattttt tcgcacatag aagcagctcc gaagagagac
     2341 gcccttcctc cctcaaccag ccgatagcct cttgcgcgtc cgaagtccag gaaacaagct
     2401 cttcggcggt gcgggttcct gtcgttcgtt taatcttccg aagtattcct agttttttct
     2461 caatgagatc cctttcgcgc tcaatttttt ccggtgagag agcggaggcc ttctcttcga
     2521 tggagctgat aaaagagtgc aggaaagaca acccttcgtt gaaagacgct tcgacgtcgg
     2581 tcttctccag aaagagacgg cgtatgagtt ccagcgcaca cgattcgagt ctgtcgatca
     2641 ttccgtcgtt ggaagcccct ccggtcaatt caaggagatt ttccctgact tttcgggcct
     2701 gttggaaggt gcggtttttt tcatcaagct ctctttccca aaggaggtcg caacccgggg
     2761 agatttgcaa cgggcgaaga gcttctacta ttgcctccgc atcgcgaaaa cgcagcttca
     2821 actcttgttc tttcgatttc accgctctca attcccggtc gcaacggagc gcttcatcaa
     2881 atgtattttc gagcgcggca cgcaaatgct cagcttgagc gccgcagcag aagtcgagaa
     2941 tatccatctg acgctttgga tcaagaagct ctatttgggc aaactggctt tgaatcctca
     3001 tcctgtcgtt cattaaagat gcaaatgtag agaagggagt cgctttgtct tgaaagaaac
     3061 atcggtttct tcctcctctt gaaagaatac gccgcgcaaa gagcagtccg tcctcgtctc
     3121 gcggttgcag cgttttcgaa actcgttcgg cagatccgga aaagacggcc tccaccgacg
     3181 cctcttcctc gccggagcga ataaaaagcg cttgactcct cttcccgccg atcagttcca
     3241 aagccctgac aaggctgctc tttccggcgc cgctctctcc ggtgatcact gtgaagccgg
     3301 tctccaggcg catcgatatg gaggctatac ctcctacgtt cttaatggag atctcttcga
     3361 tcaaccccgt cactccttct caaaggtgac aatgccctgt ccccagcgga gtttctcctg
     3421 aagcagatcg aagtaattcc gtgacgggag gagaagaaca tcgatctttt gggtgcggct
     3481 caacgagatt tctatacgat cccccggaag gatctcatag cctagctgac catcctgagt
     3541 aagaagcaaa tccctgtgat ttcctctggg aaccagggta actgtgtcat cctctcctgc
     3601 gactactggt ctggaataga gcgtatgagc gcatattggc acgacaagca tgcacggaac
     3661 gtgcgggggg ataatcggac cgcctgcgga gagggcatac gcggtcgatc cagtcggcgt
     3721 cgacacgatt atgccgtcgg ccgggagcgt gttcagaagt ttgtcgccaa tgtgcacttc
     3781 aatatgcatt accctggcaa gcgctccttt agccagg
//
LOCUS       DAOSWW010000140         3809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16812_length_3809_cov_3.521577, whole genome shotgun sequence.
ACCESSION   DAOSWW010000140 DAOSWW010000000
VERSION     DAOSWW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16812_length_3809_cov_3.521577"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /gene="glyS"
                     /locus_tag="PK442_04060"
     CDS             <1..1535
                     /gene="glyS"
                     /locus_tag="PK442_04060"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOO86852.1"
                     /translation="GIESGSTTRGHRFMGAKRIVVDDAANFMERLYDNHVILDQEKRR
                     QKMIAGISALENDLDGAVELDPEVVNENLYLVEYPIPFFGSFSKKFLEIPEEVLVTSM
                     KKNQKYFSVRGKNGKLLPYFVGVSNNLASNMNVVREGNERVLRARLEDAAFFWAEDLK
                     KPLAANVERLKTIVYQEKLGTLHDKVMATRDLALWLCAETGMKDVLQLLDRAALLAKA
                     DLVTNMVYEFPDLQGVMGREYALRNGEPERVARALYEQYLPKAAGSELPEDIIGALLG
                     LAERVHIITSCFKVGFEPTGSQDPYALRRAARCINEILWGKAMDVNLGRLVARSCEQL
                     AIQEDVRERIVAFLQQRLHVQIREKGYGHELSTLAVSVAGSRPLQVLRFLEVFSKLQA
                     EQWFVDLVTSAVRVRNILAKSGDASAAVDESLFAKEAESKLYAEIVRINPLVEEALAG
                     QDWKALAGYLSELSPFVTSFFEDVLVMDPDERVKCNRIHLLGLCNALFLKVGDLGILK
                     GV"
     gene            1570..2376
                     /locus_tag="PK442_04065"
     CDS             1570..2376
                     /locus_tag="PK442_04065"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOO86853.1"
                     /translation="MESLNLFIVSDSTGETAEHVALAAISQFNTDFANIYRYRYVEGE
                     ARIDEILAKASEAPSMVICTLVDKKSRSALAAKCAQHDTPFVDLLGPVIDALEIRLER
                     APLQAPGLLRKLDEDYFQRIKAVEFAIQCDDGRSPESLPEADLVILGVSRTGKTPLSM
                     YIANKGFRAANIPLIPEVDPPEILKTIPSSRIVGLIISGERLSQIRQERLRLLGLEPK
                     ASAYANRERIERELQTAREYLQSLGARIYDVTDRAVEETAQEILDFLRSK"
     gene            2402..3229
                     /locus_tag="PK442_04070"
     CDS             2402..3229
                     /locus_tag="PK442_04070"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOO86854.1"
                     /translation="MERVDTAPTCELFYLSDYLGEEMDSAASWITRRFEKIDIEISRY
                     GLLRTEGAVQKVLDEAARRDAFVLHGFLSGAFRRIVVERCAELGLDEYDVLRSLFSRL
                     SRIAGEEPRRDPSLVHPMDNEYFKRVKAIEYTIAADDGGNSSILKEADVVIIGVSRTG
                     KSPLCMYLAHKGIKAANIPLVPEIEPPVQLFQIEPARIIGLTRSSHSLEHIRTTRMHM
                     MGFDPDRSSYVKFERILSEIDYASGIMKRLGAVVFDVTARAIEETAHEILKLLRDGH"
     gene            3268..>3809
                     /locus_tag="PK442_04075"
     CDS             3268..>3809
                     /locus_tag="PK442_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOO86855.1"
                     /translation="MTKEKYIYSFKEGNADMKALLGGKGANLAQMVTYGLPVPPGFIL
                     TTHACLEYGKNPAFLDGIWDDVVASMKTLEKEAGKTFGTGSNPLLVSVRSGAPISMPG
                     MMNTILNLGLNDESVESLAKAADDRRFAFDSYRRFIQMYGNVVHGVDGEAFEEVLTHT
                     KKQLGLTFDNEIPAKDLEAIV"
BASE COUNT          895 a         1062 c         1084 g          768 t
ORIGIN      
        1 gtggaatcga gagcggttcc acgaccaggg ggcaccgctt catgggcgca aagcggatcg
       61 tggtggacga cgcggcgaac tttatggagc gcctctacga caaccacgtg attctcgacc
      121 aggaaaaacg ccgccagaag atgatcgccg gtatcagcgc gctcgaaaac gaccttgacg
      181 gtgcggtcga gctcgatccg gaggttgtca acgagaatct ctatctggtc gaatacccga
      241 ttcccttctt cggaagcttc agcaagaagt tcctcgaaat accggaggaa gtgctcgtca
      301 cctcgatgaa gaagaaccag aagtacttct ctgtgcgggg aaagaacggg aaactccttc
      361 cctatttcgt cggggtaagc aacaatctcg catcgaatat gaacgtcgtc cgcgagggaa
      421 acgagcgggt ccttcgcgca cgcctcgagg acgcggcgtt cttttgggcg gaggatctga
      481 aaaagcctct cgcagcaaac gtcgagcggc tgaaaacaat cgtctaccag gaaaaactcg
      541 gcactctcca cgacaaggtg atggcgaccc gcgatcttgc gctctggctc tgcgcggaga
      601 cggggatgaa agacgtgctc caacttctgg accgggccgc tcttcttgca aaggccgacc
      661 ttgtgacaaa catggtctat gagttccccg acctccaggg agtcatggga agagagtacg
      721 ccctccgcaa cggagagcct gaacgggtgg ccagggcgct ctacgaacag tatcttccca
      781 aggccgcggg gagcgagctg cctgaagata tcatcggcgc ccttctcggt ctggccgaac
      841 gtgtgcatat catcacaagc tgcttcaagg tcgggttcga acctacaggc tctcaggatc
      901 cgtacgccct ccgaagggcg gcccgctgca tcaatgagat cctctgggga aaggcaatgg
      961 atgtcaatct cgggcggctg gtggccagaa gctgcgagca gttagcaatc caggaggatg
     1021 tccgcgaaag gatcgtcgcc ttcctacagc agcggctcca cgtgcagatt cgcgagaagg
     1081 ggtacggcca cgagctttcc acgctcgccg tttcagtggc gggaagccgt ccgcttcagg
     1141 tgctgcgctt cctcgaagtc ttctccaagc ttcaggcgga gcagtggttt gtcgatctcg
     1201 tcacatcggc tgtcagagtt cgcaatatcc ttgccaagag cggcgacgcc tccgcagccg
     1261 tcgatgaaag ccttttcgcc aaggaggcgg aaagcaaact ctacgccgag atcgtcagaa
     1321 tcaatcccct cgtcgaagaa gcgctcgccg ggcaggattg gaaggcgctt gccgggtatc
     1381 tgtcggaact ttcgccattt gttacgtcat ttttcgaaga cgtccttgta atggaccccg
     1441 atgaaagggt aaaatgcaac aggatacatc ttctaggtct gtgcaacgcc ctcttcctga
     1501 aggtcgggga cctgggaata ctcaagggcg tgtagcctga gagcggcgtc aacaaaggag
     1561 tgtgaagcaa tggaatcctt gaatttgttc atcgtatccg attccactgg cgagacggcg
     1621 gagcacgtcg ctctggcggc catcagtcaa ttcaatacag attttgccaa catttaccgt
     1681 taccggtatg tcgaagggga ggccagaatc gacgaaatac tcgcaaaggc gtcggaagct
     1741 ccctccatgg tgatctgtac gctggtggac aaaaagagcc gctccgcgct cgccgccaag
     1801 tgcgcccaac acgacacccc tttcgttgat ctgctggggc ctgtcatcga tgcgctcgaa
     1861 attcgcctcg aaagggctcc gctccaggcg ccgggacttc tcagaaagct cgacgaggac
     1921 tattttcagc ggatcaaggc cgtggaattc gccatccaat gcgacgacgg acgcagcccc
     1981 gaatcgctgc cggaggcgga cctggtcatc cttggagtct cccgtaccgg gaagacgccg
     2041 ctctcgatgt acatcgcgaa caaaggattc agggcggcga atatccccct cataccggaa
     2101 gtcgatccgc ctgagattct taaaaccatc ccgtcatcga ggattgtagg gctgattatc
     2161 tccggagaaa ggctctcaca gatccgtcag gagcgtcttc gccttctggg attggaaccg
     2221 aaggcgtcgg cctatgcaaa ccgggagcgg attgaaagag aacttcaaac tgccagggag
     2281 tatctccagt cgctcggcgc ccgcatttac gacgttaccg acagggccgt cgaagagacc
     2341 gcgcaggaga tcctcgattt cctgcgttcg aaatagattg agagaagatc tttccggcag
     2401 tatggaacgt gtcgataccg cgccgacgtg cgaactcttc tacctctccg actacttagg
     2461 agaagagatg gattcggccg cgtcgtggat tacgaggcgc tttgaaaaga tcgatatcga
     2521 aatatcccga tacggtcttc ttcgtaccga aggcgccgtg cagaaagtgc tcgacgaagc
     2581 tgcccgaaga gacgccttcg tgcttcacgg attcctctcc ggcgcatttc gccgcattgt
     2641 cgtcgaaagg tgcgccgaac tcggacttga cgaatacgat gtgctccggt ctctgttctc
     2701 ccgcctctcg cgaatcgccg gagaagagcc caggcgcgat ccgtccctgg tacacccgat
     2761 ggacaatgag tatttcaagc gggtaaaggc gatcgagtac actattgccg ccgacgacgg
     2821 cggaaactcg tcgatcctga aagaagccga cgtcgtcatc atcggcgttt ccagaaccgg
     2881 gaagtcccct ctgtgcatgt accttgccca caagggaatt aaagctgcca acataccgct
     2941 cgtccctgaa atcgagcccc cggttcagct ctttcagata gagccggcaa gaattatcgg
     3001 cctgacacgt tcgagccatt ctctggaaca tatccggacg acgcgtatgc acatgatggg
     3061 gttcgatccg gacagaagca gttacgtaaa attcgaacgg attttgagtg aaatagacta
     3121 cgcatcgggt ataatgaaac gtcttggagc ggttgttttc gacgtcactg ccagagctat
     3181 agaggagacg gcgcacgaaa tactgaagct gcttcgcgac ggacactgaa agtatcttat
     3241 ccaaacctac ggaggtgcag tatagagatg acgaaagaaa agtacatcta cagtttcaaa
     3301 gaaggcaacg cggacatgaa ggcgctcctc gggggcaaag gggccaacct cgcccagatg
     3361 gtgacgtacg ggctcccggt tcctccgggc tttattctga cgacgcacgc atgcctcgaa
     3421 tacggcaaga atccggcatt tctcgatgga atatgggacg acgttgttgc gtcgatgaaa
     3481 acgcttgaga aagaggccgg aaaaacattc ggcacaggaa gcaatccgct tcttgtctcg
     3541 gtccgttccg gcgcgcccat ttcgatgccc ggaatgatga acaccattct caatctcggc
     3601 ctgaacgacg agtccgtcga atcgcttgcc aaagccgccg acgaccgccg tttcgcgttc
     3661 gacagctacc gccgctttat ccagatgtac ggcaacgtgg tgcacggcgt cgacggcgag
     3721 gcgtttgagg aagtcctgac gcacacgaag aagcagctcg gtctcacgtt cgacaatgaa
     3781 atccctgcga aggatctcga agccattgt
//
LOCUS       DAOSWW010000141         3809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16814_length_3809_cov_3.001598, whole genome shotgun sequence.
ACCESSION   DAOSWW010000141 DAOSWW010000000
VERSION     DAOSWW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16814_length_3809_cov_3.001598"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /locus_tag="PK442_04080"
     CDS             <1..1522
                     /locus_tag="PK442_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOO86856.1"
                     /translation="PAEALFDGIDYCPAHPAVLLGHHFASIAGAGPIVGPITAAAMFG
                     WLPAYLWCLIGSAFLGGPHDMGALVASMRHDGKSVGEVVDKWIGRKGKILFLCFTILA
                     LVLVVAVFLQLSAGSFAADPAVAFSASLYIGMALLFGVLIYKYHVPLWLMTVVMVPIV
                     IYACWYGNQAAWVAEMFKLPMETWRWILAGYIFFASVLPVWLLLQPRDYLASYFLYFA
                     VIIGTIGMLMGKGEGFEVVLPAFKGFVAGNQFLWPMLFVIVACGAISGFHSLVGSGTT
                     SKQLRKEKDTVLVGYGSMLLEGLVAVIAIATIMITGTIAQGGPTITYAQGFGKFAAII
                     GIDPKVGMSLGLLAINSFLLTSLDTATRLTRYQIQELSNMKIDKYTATVIAVAAGMAL
                     LLTKAHGPTGNVIPAWAAIWPIFGASNQLVAALALLTIGVWVGKALKKDNRFAMYPMW
                     FMLVTTVAALGFLIKDNMAYEHPNYILVVPSIILLVLAIMMVCESLKALKNPDIKA"
     gene            1978..3723
                     /locus_tag="PK442_04085"
     CDS             1978..3723
                     /locus_tag="PK442_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOO86857.1"
                     /translation="MIFPFSGEVKEVSCVLSGAAGLGIQTVEDMLARIVVDSGFSVFG
                     SREYMSRVRGGNNSTELRIAPFRVDALVDRMDILVALSRNVRENIRERITPSTLIIGD
                     KEELKGELEDAGALFVDVPLSRLAAEAGGRIYSNSVAAGILLGILGLGTEKAEAFFEE
                     RFSGKKGDVAGKNTMACRSGYELGEKLVKGKGLLSGMQADLQVGKALINGSDAVSLGA
                     MAAGCNFVTAYPMSPATGVLSFFAKHAEKAGAAVEQVEDELAAINMAVGASYTGARPL
                     VTTSGGGFALMGEGLSLAGVMETPVVVHLSQRPGPATGMATRTEQADLELALYSGHGE
                     FPRAIYAPGSIESAFSLTQEAFRVALKYQTPAIVLTDQYFVNSFYNVRPDDLFFSEPP
                     SPVRETGEGYRRYEETASGCSPFGVPGWGSGIVGADSHEHDEVGHVYENFKLRKRMQE
                     KRMRKLEGMREEALAPKLYGAEEFTDLVLCWGSTIPIFKEALTLLDLKQTALLAFEQV
                     WPLHSSVDALLKRARRIVAAEGNSTAQFARLLRAQTGVAATDTVLKYNGLQFSVEEAA
                     SRLSEVFSGRGGRER"
BASE COUNT          744 a          971 c         1106 g          988 t
ORIGIN      
        1 tcccgccgaa gcgttgtttg acggaatcga ctactgcccg gcccatcctg cggtgctttt
       61 gggccatcac ttcgcgtcca ttgccggcgc cggtcccatt gtcggcccca ttacagcagc
      121 cgctatgttc ggctggcttc ccgcctacct gtggtgtctc atcggttccg ccttccttgg
      181 cggcccgcat gacatgggag cgcttgtcgc ttcgatgcgc catgacggaa agtccgtcgg
      241 cgaagtcgtc gacaagtgga ttggccgcaa agggaaaatc ctcttccttt gcttcacgat
      301 ccttgccctt gttcttgtgg ttgcagtatt cctgcagctt tccgccggtt ctttcgcggc
      361 ggatcccgcc gttgcttttt cagcctcgct ttatatcggc atggctctgc tttttggtgt
      421 acttatttat aagtatcacg tccccctgtg gctcatgact gtcgtcatgg ttcccattgt
      481 tatttatgct tgctggtacg gtaatcaggc tgcatgggtc gcggaaatgt tcaagcttcc
      541 tatggagacg tggcgctgga ttcttgccgg gtatattttc tttgcctctg ttctgccggt
      601 atggcttctt cttcagcctc gcgactatct ggcatcctat ttcctttatt tcgccgtcat
      661 catcggaacg atcggcatgc tgatgggcaa gggcgagggc tttgaggtag tacttcccgc
      721 tttcaagggc ttcgttgccg gcaaccagtt cctttggccc atgctctttg ttatcgttgc
      781 gtgcggcgct atctcgggct tccactctct ggtcggcagc ggaactacct ccaagcagct
      841 gcggaaagag aaggacactg tgcttgtcgg atacggctct atgcttcttg aaggtctggt
      901 agcggttatt gctatcgcaa caatcatgat taccggcacc atcgcgcagg gcggcccgac
      961 gatcacctat gcgcagggat tcggaaagtt cgccgcgata atcggcatcg acccgaaggt
     1021 cggtatgtct ctcggccttt tggctattaa ctccttcctt ctgacgtcgc ttgatacggc
     1081 aacccgtttg acccggtacc agattcagga gctttcgaac atgaagatcg acaaatatac
     1141 cgccaccgtg atcgcagttg ccgccggtat ggctctgctt cttacgaagg ctcacggccc
     1201 cacgggaaac gtaattcccg cgtgggcggc gatctggccg atcttcggcg cttccaacca
     1261 gcttgttgcg gcgcttgcgc tgttgactat cggcgtttgg gttggtaagg ctctgaagaa
     1321 ggacaatcga ttcgcgatgt acccgatgtg gttcatgctt gtcacaactg ttgcggctct
     1381 tggtttcttg atcaaggaca acatggcgta tgagcatcct aattacatct tggttgttcc
     1441 ctccattatc cttttggttc tggcgatcat gatggtctgc gaatctctga aggcgcttaa
     1501 aaatccagat atcaaggcgt agctgagctc tagttcaagg gttgtacaga aacgaacgct
     1561 gcaaaaatag aggaaggcgc cggttttttc ggcgccttcc tctatttggc cttttagaca
     1621 gattattttg tgcttttccc tacgctgaag gattttgcgc tccaggattt gccccgtttc
     1681 ctgttttcgg cgtcaattac ctcaaggaat ctgctgccac acctctggca tcgcatggtc
     1741 gagttttttt cgtccaactc gaattcgctt ttgcacgata gacatctaaa tttccccata
     1801 aaaacacctc cgtattttct tggaaaatca aagttaccct atatccatcg tccatacttc
     1861 atgagatatt atagcatgtg gtcgaaaaga agtggatttg ctgaacgagc gcttcgggaa
     1921 gtagaaagct gtataattta aaacagggta acgtactttt ttgaagggag gacgactatg
     1981 atctttccat tttcgggaga ggtgaaggaa gtatcctgtg ttctctccgg cgctgctggt
     2041 ctgggaattc aaacagttga agacatgctc gcccgtattg tcgttgattc cggtttttcc
     2101 gttttcggat cacgcgagta catgtccagg gtaagaggag ggaacaactc gacggagctg
     2161 cgtatagcgc cgtttcgtgt cgatgcgctt gtggacagga tggatatcct ggtggccctg
     2221 agccggaacg tgcgggagaa tattcgagag cgtatcacgc cttccacgct gatcatcggc
     2281 gataaggagg aactgaaggg agagcttgag gacgccggag cgctgtttgt tgatgtcccg
     2341 ctttcccggc ttgcagccga ggcaggaggt cgtatttact cgaattctgt agcggcgggt
     2401 attcttctgg gaattcttgg gctgggaacg gagaaggcgg aagcattctt cgaggagcgt
     2461 ttctccggga agaaaggcga tgtcgcgggg aaaaatacga tggcctgccg gtcggggtac
     2521 gagctcggcg agaagctggt aaaggggaag gggcttctct cgggaatgca ggcggatctt
     2581 caagtcggga aggctctgat caacggaagc gacgcagtct ctttgggggc tatggccgcc
     2641 gggtgtaatt ttgtgacagc ctacccgatg tctccggcca ccggcgtgct ctccttcttc
     2701 gcgaagcatg cggagaaggc tggcgctgcc gtagagcagg tagaggacga actggctgcg
     2761 atcaacatgg ctgtaggcgc ctcctatacc ggcgcgcgtc ccttggttac cacgtcagga
     2821 ggcggctttg cccttatggg cgagggactg agtttggcag gtgtcatgga gactcctgtt
     2881 gtagtccatc tttcgcagcg gccaggtcct gctaccggga tggcgacgag aacagaacaa
     2941 gcggatctcg aacttgcgct gtactcgggg cacggcgaat tcccacgggc gatctacgcg
     3001 ccggggagca tcgaaagcgc tttctcgctg acacaagagg cgttcagggt cgcgctgaag
     3061 taccagacgc ccgccattgt gcttaccgat cagtattttg tgaattcttt ctataatgtg
     3121 cgccccgacg acttgttttt ctcggaaccg ccgtctcctg tccgggagac cggggagggg
     3181 tacagacgct atgaagagac cgcatccggc tgttccccgt tcggcgttcc ggggtggggg
     3241 agcggtatcg tcggcgcgga cagtcatgaa cacgacgagg tcggacacgt ctacgagaat
     3301 ttcaagcttc ggaaacgaat gcaggaaaaa cggatgagaa agcttgaggg catgcgtgaa
     3361 gaagcgcttg cgccgaagct gtacggggcc gaggaattta cggaccttgt actctgttgg
     3421 gggtcgacga ttccgatttt caaggaggcg ttgacgctcc tggatttgaa gcaaacagct
     3481 cttctggctt tcgaacaggt ttggccgctt cattcctcgg tagatgcgct gttgaaacga
     3541 gctcgtcgca tcgtcgccgc ggagggaaac agcactgcgc agttcgcgcg cttgctccgc
     3601 gcgcagactg gcgttgccgc gacggacacg gtattgaaat ataacggcct tcagttctcg
     3661 gttgaagagg ctgcgagcag actgtcggaa gttttttccg gaagaggagg gagagagcga
     3721 tgagtacgtc cttcgacctt cccggcgtcg aaatttcctg gtgtccgggg tgcggcgatt
     3781 ttaaaattct cgatgcaatc aaacaggcg
//
LOCUS       DAOSWW010000142         3807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16825_length_3807_cov_4.008262, whole genome shotgun sequence.
ACCESSION   DAOSWW010000142 DAOSWW010000000
VERSION     DAOSWW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16825_length_3807_cov_4.008262"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PK442_04090"
     CDS             complement(<1..263)
                     /locus_tag="PK442_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOO86858.1"
                     /translation="MTVHLVGAGCGGPLWLTLKGKALLEGAEHIVYDSLIHPDLLQLA
                     PRACAFHPVGKRKGRPSPKQSEISALLVELGQTGKSVVRLKGG"
     gene            complement(260..1252)
                     /gene="hemC"
                     /locus_tag="PK442_04095"
     CDS             complement(260..1252)
                     /gene="hemC"
                     /locus_tag="PK442_04095"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOO86859.1"
                     /translation="MKLLTRGSTLALVQTERVASSLRALGIDVDIERVSTKGDRDTIS
                     PLCSFGGSGAFSSCIEEALLRGMGDGAVHSLKDLPSCCREGLEIAAVCPRDSVEDLLL
                     CREEQNLENLPRGAVVGTSSPRRRAQLLRRRPDLSVRELRGNVATRLQKLHDGLYDAI
                     VLAHAGLERLDIRTPNTTPLPFLPAPCQGIIALEAPLNGALFPLGERITDRETHLCSI
                     AERSLLRTLGVGCHVPFAALAELHGETMLLRAEILECDGRDSVSLTFERTVGTDEEAL
                     DAGRSLGGLFRNEPRAARLLKACAVPPPPRKEERTQRAGGPSVCTAALAGGDLR"
     gene            complement(1249..1743)
                     /locus_tag="PK442_04100"
     CDS             complement(1249..1743)
                     /locus_tag="PK442_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO86860.1"
                     /translation="MICLDASAGPILVVGGGATGLRKIRTLLDAGMAVTLISPEIVPE
                     LEEIVREGRVAWERRIAERRDFEEHVFALLALPRSESETVLTLAEGTRCLVNCCGAGD
                     LGAWSLAAQFRVASSVAALPEDVPSPAESHWYTVGVASGGRSPAGSAALKRRLQHSLE
                     GDRS"
     gene            complement(1781..3040)
                     /locus_tag="PK442_04105"
     CDS             complement(1781..3040)
                     /locus_tag="PK442_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86861.1"
                     /translation="MTRLVCISLDYTAANTAERASAWNEDRLRALKDEGRILEGLLLT
                     TCNRSEVYLRLPPDAETPSELTHPKARVYSGTELSLHLLRVLLGLESLACGESHIAAQ
                     VKACYAEGNDLCGPWLHRLFQSSLKMAKLLRTCYHPGMEPSIPRLMVDMLAPSSPPSP
                     LSVLVLGAGEMGEETSRLLLSLPLSGSPQREAKAATDGEAAPRAALKPAVGSLVLSNR
                     TPDRTEALFSRLLPRLERESGPKVATLPWEQWREASRRFDAIFFCTGSPVPLFSMEDA
                     RPGQQIFDLGSPPQVVRSAEVAGARLVAIDDLARTAEEATKRYRKKLCRLEEEATNAA
                     VSVMSELETLSGETYKRLVLHRAERIVADRAERTSKRTGTDEETLRRMGWSIVKALLS
                     PAFAESDPHARRLWKILAGELEESDDV"
     gene            complement(3037..>3807)
                     /locus_tag="PK442_04110"
     CDS             complement(3037..>3807)
                     /locus_tag="PK442_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HOO86862.1"
                     /translation="PETGTKRGALFILGTGPGVGGSVTIDARQALEEADAVVGYALYV
                     DLLPQEWLQGKVVERYSMGEEEKRVERAVALAEEGNVVALLSGGDPVLFGLAGLALRT
                     ANGRVPAKVFPGVTAAQAAGALLGAPYVNGVALLSLSDYLQPWSAVLRALEGAALSGL
                     TVALYNPVKRDLEEKLREVRRVFSAQGYETAYLVRDAGRDAPSVRKLPLAELGADHLD
                     MRTLILLPGSSVEEFAGLLLDRRGYRRETDKHKEESAQ"
BASE COUNT          623 a         1250 c         1325 g          609 t
ORIGIN      
        1 tcgccgccct tgagccggac gacgctcttc cccgtttgtc ccaactcgac gaggagcgcg
       61 ctgatctcgc tctgtttcgg cgacggcctc cccttgcgct tcccgaccgg gtgaaaggcg
      121 cacgcccgag gggcgagctg aaggaggtcg gggtggatca ggctgtcgta cacgatatgc
      181 tcagcgcctt caagcagcgc tttccccttc agcgtcagcc agagaggccc gccgcacccc
      241 gcgccgacaa gatggacggt catcggagat cgcctccggc gagagcggcg gtacagaccg
      301 aaggcccccc ggcgcgttga gtgcgttctt ccttcctagg gggaggcgga acggcgcacg
      361 ccttcagcag acgggccgca cgcggttcgt tcctgaagag accgccgagc gaacgcccgg
      421 cgtccagcgc ctcttcgtcc gtcccgaccg ttctctcgaa ggtcagagaa accgagtccc
      481 tgccgtcgca ctcaaggatc tccgcgcgca gcagcatcgt ctctccgtga agctccgcca
      541 gcgcggcgaa ggggacgtgg cagccgacgc cgagcgtccg gagcagggat cgttcggcga
      601 tcgaacagag gtgcgtctcc ctgtcggtga tccgttcacc caacgggaag agcgcgccgt
      661 tcaggggcgc ttcgagggcg atgattccct ggcagggcgc gggaaggaac ggaagcggcg
      721 tcgtgttcgg cgtccggatg tctaggcgct cgagcccggc gtgcgcgagg acgatcgcgt
      781 cgtagagtcc gtcgtgcagc ttctggaggc gggtggcgac gtttccgcga agctccctca
      841 cggagaggtc ggggcggcgg cgcagcagct gggcgcgtcg gcgggggctt gaagtgccca
      901 cgaccgcgcc gcggggaagg ttctccaggt tctgctcctc acggcagagc aagagatcct
      961 ccacggagtc gcgggggcag acggcggcga tctccaaccc ttcgcggcag catgagggga
     1021 ggtccttcag gctgtggacc gctccgtcgc ccatgccgcg cagcagcgcc tcctcgatgc
     1081 agctcgaaaa cgcccccgag ccgccgaagg agcagagcgg actgatcgta tcccggtccc
     1141 ccttggtgga gacgcgctcg atgtcgacgt cgatcccgag cgcacgaaga gaggacgcga
     1201 cccgttcggt ctggacgagg gcaagggtgc ttccgcgtgt gagcagtttc atgaacggtc
     1261 tccttccaga gagtgctgca gtcggcgctt cagcgcggcg gagccggccg gagagcgtcc
     1321 gccgctggcg acgccgactg tgtaccagtg gctttcggcg ggagagggaa cgtcttccgg
     1381 aagcgccgcg acggaagatg caacgcggaa ctgggcggcg agcgaccacg ccccgaggtc
     1441 accggcgccg cagcagttca cgaggcagcg cgtcccctcg gcgagggtga ggaccgtctc
     1501 agattcggag cgggggagcg cgagaagcgc gaagacgtgc tcctcgaagt cccgccgttc
     1561 ggcgattctc cgttcccagg cgacgcgccc ctcccgcacg atctcctcca gctccgggac
     1621 gatctcggga gagatcagcg ttacagccat tcccgcgtcg agcagcgtcc ggattttgcg
     1681 cagtccggtc gcgccgccgc cgactacgag tatcggtcct gcggaggcgt cgagacagat
     1741 cataagggag gctcgctccc gggagcgccg cgacggagcg ttacacatcg tcggactcct
     1801 ccagctcgcc cgcgaggatc ttccagagcc ttcgggcgtg ggggtccgac tcggcgaaag
     1861 cgggggagag gagcgccttc acgatgctcc accccattct gcggagcgtc tcctcatcgg
     1921 ttcccgttcg cttcgaggtc cgctcggccc tgtccgcgac gattcgttca gccctgtgga
     1981 gaaccagtcg tttgtacgtc tcgcccgaga gcgtttcgag ctccgacatg accgagacag
     2041 cggcgttcgt cgcctcctcc tccagtctgc aaagcttctt cctgtagcgc ttcgtcgcct
     2101 cctccgccgt ccgggcgaga tcgtcgatgg cgacaaggcg ggcgccggcc acttccgcgg
     2161 agcgcacgac ctgcgggggc gaaccgaggt cgaagatctg ctgtcccggt cgcgcatcct
     2221 ccatggagaa gaggggaacg ggggagccgg tgcagaagaa gatcgcgtcg aaacgcctcg
     2281 acgcctcgcg ccactgttcc cagggcagcg tcgcaacctt tgggccggat tcccgctcaa
     2341 ggcgcggcaa gaggcgggag aacaacgctt ccgtgcggtc gggagtccgg ttcgacagga
     2401 cgagcgaacc aacagccggt ttgagggcgg cgcggggcgc ggcttcgccg tccgtcgcag
     2461 ctttggcttc ccgttgcggc gagccggaga gcggcagtga aagaagcagc cgggaggttt
     2521 cttctcccat ctcgcccgcc ccgaggacga ggacggaaag aggagaaggc ggggaggaag
     2581 gggcgagcat atcgaccatc aggcgcggaa tcgagggctc catgcccgga tggtagcagg
     2641 tccgcagcag ctttgccatc ttgagggagc tttggaacag acgatggagc cacgggccgc
     2701 agaggtcgtt tccttctgcg tagcacgcct tgacctgcgc cgcgatatgc gactcgccgc
     2761 aggcgaggct ctccagcccc aggaggacgc gcagcaggtg gagggaaagc tccgtcccgg
     2821 aatagacgcg agccttcggg tgcgtcagtt cggacggcgt ctcggcgtcc ggcgggaggc
     2881 gcaggtagac ctccgaacgg ttgcatgtgg tcagcagaag tccctcaagg atccgcccct
     2941 cgtctttcag ggcgcggagc cggtcttcgt tccatgcaga agcgcgttcc gccgtgtttg
     3001 ccgcggtgta gtcgaggctg atgcagacaa gccgcgtcat tgcgcggact cctccttgtg
     3061 cttgtccgtc tccctgcgat atccgcgtct gtcgaggaga agaccggcga actcctccac
     3121 ggagcttccc ggaaggagga tcagcgtgcg catgtcgagg tggtccgcac cgagttcggc
     3181 gagcggcagc ttccggacgg agggcgcgtc ccgtcccgcg tcgcggacca ggtacgccgt
     3241 ttcgtacccc tgtgccgaga agacgcgacg gacctcgcgg agcttctctt caagatcgcg
     3301 cttcacgggg ttgtagaggg cgacggtgag cccggagagg gccgctcctt cgagggcccg
     3361 gaggactgcg ctccacggct ggaggtagtc cgacagtgag agcagcgcga cgccgttgac
     3421 gtagggagcg cccagcagcg cgccggcggc ctgcgccgcg gtaacgccgg gaaagacttt
     3481 cgcgggaacc ctgccgtttg ccgtgcgcag cgcgagcccg gcgaggccga agaggacggg
     3541 atccccaccg gagaggagcg cgacgacgtt cccctcctcg gcgagcgcaa ccgcgcgttc
     3601 cacccgcttt tcctcctccc ccatggagta gcgttccacg acctttccct gtaaccactc
     3661 ctgcgggagc agatcgacgt agagggcgta gccgacgacg gcgtccgctt cctccagcgc
     3721 ctgtctggcg tcgatcgtga cggaaccgcc cactcccggg cctgtgccga gaatgaagag
     3781 cgcgccgcgc ttcgtccccg tctccgg
//
LOCUS       DAOSWW010000143         3805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16838_length_3805_cov_4.294400, whole genome shotgun sequence.
ACCESSION   DAOSWW010000143 DAOSWW010000000
VERSION     DAOSWW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16838_length_3805_cov_4.294400"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PK442_04115"
     CDS             <1..878
                     /locus_tag="PK442_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO86863.1"
                     /translation="AGFMAIGAYVCSILILSPAQKEAMWILEPLIWPLSVIKAPFFVA
                     VLSGGLIAAFFALLIAIPVLRLGGDYLGIATLGFAEIIRVVLTNLSFITNGALGIKGI
                     PPYANIWWNYAWLLLTLFFIIRLVNSNFGNVLKAIRDDEIAAKNMGINTFFYRVLAFT
                     IGAFFAGVGGALMGSLITTIDPRMFMFVLTFNILMIAVAGGLGSITGSIIGSIVITIL
                     LEWLRFVENPLVIGSFEIPGIPGMRMVIFSLALILIILFRREGIMGMREITWDTFFSR
                     GRRSPEHEEKGGKMK"
     gene            875..1669
                     /locus_tag="PK442_04120"
     CDS             875..1669
                     /locus_tag="PK442_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO86864.1"
                     /translation="MTTPASILRTENLTMRFGGLTAVGNFSIDVPQGKIVGLIGPNGA
                     GKTTVFNMITGFYRPTEGRIFFDGQDITGFQPHMVCRSGISRTFQNIRLFTNETVLEN
                     VMIGCHVRRKSKWWMAPLGLPSFRAEEEAIREKSMDLLKSVGLEDFADEPSASLSYGA
                     QRRLEIARALATDPKFLLLDEPAAGMNPQESQELMSFIRNIQKRFNLTILLIEHDMKV
                     VMGVCEYIWVLDYGVKIAEGNPDAIQSDQKVIVAYLGEEYVKYVEN"
     gene            1656..2384
                     /locus_tag="PK442_04125"
     CDS             1656..2384
                     /locus_tag="PK442_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO86865.1"
                     /translation="MLRIENLHVYYGAIHAVKGINLEIPEGAIVTLIGANGAGKSSTI
                     RAVAGLVKNVKGKILYTPSGGDAAGQVNLIGKTPEEMVKLGIAMSPEGRRILPHLTVE
                     ENLQLGAFIRNDKDGIERDRDHVYELFPRLKERSWQKGGTLSGGEQQMLAVGRALMSQ
                     PKLVMLDEPSLGLAPLLVKEVFEIIGEINALGKTVLLVEQNAFAALKIASYAYILEVG
                     NIVLEGKGEELLKDARVKEAYLGG"
     gene            2494..3063
                     /gene="pgsA"
                     /locus_tag="PK442_04130"
     CDS             2494..3063
                     /gene="pgsA"
                     /locus_tag="PK442_04130"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOO86866.1"
                     /translation="MGKKVWNIPNTLSLLRVFLAPLVLIFLTFKFTYPVYSVEDLGIL
                     VTVGDVLAGSVFILAALTDTADGYIARKKGMVTNLGKFIDPLADKILVMAALIALVEL
                     HRIPAWIVVVILTREMIVTGVRMVAAAEGIVIAASNAGKLKTVFQIVAIAMIIFNIPF
                     GIHVMWVAMLMTLWSGMDYLIKGKKLFTE"
     gene            3204..>3805
                     /locus_tag="PK442_04135"
     CDS             3204..>3805
                     /locus_tag="PK442_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOO86867.1"
                     /translation="MLEMLKKALGLDPNERALKRYTSVVEQINALEPALLSLSDEELR
                     EKGASFKKRLTEGETRDDMLPEVFAVVREVSRRTIGLRHFDVQLMGGMALHEGKITEM
                     KTGEGKTLVATLAVVLNALSGEGVHLVTVNDYLARRDAEWMGPIYTFLGLSVGVIYAY
                     MDPEERLASYRADITYGTNSEFGFDYLRDNMAVNESHLVQ"
BASE COUNT          865 a          943 c         1053 g          944 t
ORIGIN      
        1 atgcggggtt catggcaata ggcgcatacg tatgctcaat tcttattctt tccccggccc
       61 agaaagaggc tatgtggatt cttgagccct tgatatggcc tctttcagtc atcaaggcgc
      121 cgtttttcgt cgcggtgctt tcgggcggtt tgattgcggc tttcttcgcg ctccttatcg
      181 ccatacctgt tctgcgcctt ggcggagatt accttggcat cgccacgctt ggcttcgcgg
      241 agatcatccg tgtggtgctc acaaacctga gctttattac caacggcgcc ctcggtatta
      301 agggaattcc cccctatgcg aatatctggt ggaattatgc ctggctcctt ctgactctct
      361 tctttatcat ccgcctcgtg aacagtaact tcggaaatgt cctgaaagcg atccgcgatg
      421 atgaaatcgc agcgaaaaac atgggaatca ataccttctt ctacagggtg ctcgcgttca
      481 caataggcgc tttttttgcc ggtgtaggcg gcgctctcat gggaagtctc atcaccacga
      541 tagatccgag gatgttcatg ttcgtcctca cgttcaatat cctcatgatc gcggttgcgg
      601 gaggtttggg gtccattacg ggcagcatca tcggaagtat cgtcatcacc attcttcttg
      661 aatggcttcg ctttgtcgag aaccccttag tgatcggctc ctttgagatt ccgggtattc
      721 ctggaatgag aatggtgatc ttttccctcg cgctcattct tatcatcctc ttccgcagag
      781 aggggattat gggaatgcgt gagataacat gggatacgtt tttctctcgc ggaagacgaa
      841 gccccgaaca tgaagaaaag ggaggaaaaa tgaaatgacg actcccgcat ccatacttcg
      901 gactgagaat ctcaccatgc gcttcggagg cctcactgcg gtggggaatt tcagcatcga
      961 cgtgccgcag ggaaaaatag tcggtcttat cggaccgaac ggggcgggca agacgaccgt
     1021 tttcaatatg attacaggct tttaccgtcc taccgaagga cgtatcttct ttgacggaca
     1081 ggatataacg gggtttcagc cgcatatggt ctgccgttcg gggatatcga ggacattcca
     1141 gaatatccgc ctcttcacaa acgagacggt tctggaaaac gtcatgatcg gctgtcacgt
     1201 ccgcagaaag tcgaaatggt ggatggctcc gctggggctt ccctcgttca gggcggaaga
     1261 ggaggccatt cgcgaaaaat cgatggatct cttgaagtct gtgggcttgg aggattttgc
     1321 ggacgaacct tcagcttccc tctcctacgg agcgcagcgc cgccttgaga ttgcacgcgc
     1381 tcttgcgacc gatcccaaat ttctgctgct cgacgaaccc gcagccggta tgaatccgca
     1441 ggaatcgcag gagctcatgt cgttcatccg gaatatccag aagcgcttta acctgaccat
     1501 ccttcttatc gaacacgata tgaaagttgt catgggcgtc tgcgagtata tctgggttct
     1561 tgactacggc gtaaaaatcg ccgagggcaa ccccgacgct atccagtcgg accagaaggt
     1621 gatcgtggcc taccttggag aggagtatgt aaaatatgtt gagaattgag aatctccatg
     1681 tgtattacgg cgccattcac gctgtgaaag ggatcaacct tgaaattcct gaaggcgcca
     1741 tcgtcactct tatcggagcg aacggcgcgg gaaagagcag caccatccgc gctgtggcgg
     1801 gacttgtaaa aaacgtcaag gggaagattc tctacacgcc ctccggcggc gatgctgcag
     1861 ggcaggtaaa tcttatcggc aagactccgg aagaaatggt gaagcttggg attgcaatga
     1921 gtcccgaagg gcgtcgtatt ctgccacacc ttactgtgga ggaaaatctt cagctcggcg
     1981 cctttatccg gaatgacaag gacggaatag aacgagacag ggatcatgtc tacgagctct
     2041 tcccccggtt gaaagagcgc tcttggcaga agggcgggac tctgtcggga ggagagcagc
     2101 agatgctcgc cgtgggccgc gcgcttatga gccagccgaa gctcgttatg ctcgacgagc
     2161 cctcgctggg actcgcgccg ctccttgtga aagaggtctt cgaaataatc ggcgagataa
     2221 acgctctggg aaaaacagtg ctccttgtgg agcagaacgc ctttgcagcc ctaaagatcg
     2281 cctcctacgc ctatatactt gaggtcggga atattgtatt ggaaggcaag ggagaagagc
     2341 tcctcaagga tgcccgtgta aaagaggcct atctgggggg ataaattgtt tttttatgca
     2401 gtgagaaggg tagccgggac tttttccggt tgcccttttt tgctttatca actacactgt
     2461 acacgcaaat catctttcag gaggaacatt ttcgtgggaa aaaaagtatg gaatatacct
     2521 aacacactca gtctccttcg tgtttttctc gcgcctctcg ttttgatttt cctgacgttc
     2581 aaatttacgt accctgtcta cagtgtcgaa gatctcggga ttttggtgac ggtcggcgat
     2641 gttttggccg gctccgtgtt tattctcgca gccctgacgg atacggccga cggctatatc
     2701 gcccggaaga aaggaatggt tacaaacctt ggcaagttta ttgatccgct cgcggacaag
     2761 atacttgtca tggcggctct tatagctttg gtcgagcttc accgtattcc ggcctggata
     2821 gtggttgtca ttctcacccg ggagatgatc gtcacgggag tcaggatggt cgccgcagct
     2881 gaaggcattg tcattgcggc ctccaacgcg ggcaaactga agacggtctt tcagatcgtg
     2941 gcaattgcga tgatcatctt caacattccc ttcggaatcc atgttatgtg ggtggctatg
     3001 ctgatgacgc tctggtcggg gatggactat ctgataaagg ggaaaaaact ctttaccgag
     3061 tagccgtgcg tttttcagtc ctgacgcgcc gtttccggga ggtccgcata aatactttga
     3121 ccacgacggg ttcgggacat ataatgaccc ggcggtatgt gccgtgcgcg ctgcacggcc
     3181 gaattttttg ttgaggtgac gctatgctcg aaatgctcaa aaaggccctc ggtctggatc
     3241 ctaacgaacg ggccctgaag cgctatacaa gtgtcgtaga acagatcaat gctcttgagc
     3301 ctgccctttt atcgctctcc gacgaagagt tgcgcgagaa aggcgcttca tttaaaaaac
     3361 gcctcacgga gggtgaaacc cgtgatgata tgctcccgga ggtcttcgcg gtagtgagag
     3421 aagtgtccag gcgcaccatt ggactgcgtc atttcgatgt ccagctgatg ggtggtatgg
     3481 cgctgcacga gggaaagatc accgagatga agacgggaga gggaaaaacg ctcgttgcaa
     3541 ctcttgccgt tgtgttaaac gctctttcag gggaaggagt tcacctggtg acggtgaacg
     3601 actacctcgc cagaagggac gccgagtgga tggggccgat ctacacattc ctcggactct
     3661 ccgtcggcgt catttatgcg tacatggacc cggaagagcg tcttgcctcc tacagagcgg
     3721 atatcacata cggaacaaac agcgaattcg gtttcgacta tctgcgcgac aacatggcgg
     3781 tgaacgagtc gcatcttgtg cagag
//
LOCUS       DAOSWW010000144         3800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16874_length_3800_cov_3.225901, whole genome shotgun sequence.
ACCESSION   DAOSWW010000144 DAOSWW010000000
VERSION     DAOSWW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16874_length_3800_cov_3.225901"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK442_04140"
     CDS             complement(<1..294)
                     /locus_tag="PK442_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86868.1"
                     /translation="MIIGRDDLRSAAKKGILSEEQADSLWDFLSEEMREVPSFRMAHI
                     LYYLGGLIAIGAMTLFMNRGWELFGGWGIFAISLAYALVGLFFLAYFMKKPGLS"
     gene            495..920
                     /locus_tag="PK442_04145"
     CDS             495..920
                     /locus_tag="PK442_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO86869.1"
                     /translation="MIAKKGSKVKVHYTGTLKNGDVFDSSEGRSPLEFTVGAGQMIPG
                     FDKGVEGMNAGEKKTITLPPKEAYGEKRDDMLFRVSRDVLPEGYTPSIGDPLRVATDD
                     GGVLNVFIAEMDEDGITLDGNHRLAGEELTFEVTMVEIA"
     assembly_gap    1466..1475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1476..2420
                     /locus_tag="PK442_04150"
     CDS             <1476..2420
                     /locus_tag="PK442_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HOO86870.1"
                     /translation="IPAWIVSPKKEYSAATFRRKVHPLLDSYLEEYPLFPELPPRESI
                     ETPLFLSLEDAAARVATETAEAPLSPYSPGSSAARAAMRRFFSERLNDYDTRRNNPAL
                     SALSDLSPWLHFGQLSPQRVALEALKTPQSPAREAFLEELIVRRELADNFCFYEPMAG
                     TYDGLPEWGRKTLALHAGDRRPRLYTMAELEEARTGDPLWNAAQKEMVWKGKMHGWAR
                     MYWAKKILEWTETPEEAFERALILNDRYELDGRDPSGVVGVSWAIGGLHDRPWAERSV
                     FGMVRYMNDKGAARKFRVADYIESVNAFISNRTGENLS"
     gene            complement(2640..3230)
                     /locus_tag="PK442_04155"
     CDS             complement(2640..3230)
                     /locus_tag="PK442_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOO86871.1"
                     /translation="MRYKGWILAGFFAGLTVVGTFLRIPFPLVPLTLQVLMVLLAGFT
                     LGPKYGMLSQVIYLFIGLAGLPVFSGGGGIGTVFSPTFGYLLSYPAAAWIVGRISSKE
                     GANAIRFVVASCAGIAFIYLLGASVLYLNLKFLAGKSIGVYQVVKIGVLPFLLPDLLK
                     GGVAALFALKIRPLLGTSGAELRRAPLHGRLTRYKP"
BASE COUNT         1011 a         1053 c          920 g          806 t
ORIGIN      
        1 ggagaggccg ggttttttca tgaaatacgc cagaaagaag agacctacaa gcgcataggc
       61 aagtgagata gcaaagatgc cccatcctcc gaaaagttcc caaccccgat tcatgaagag
      121 cgtcatcgca ccgatcgcaa tcagtccgcc aagatagtag agaatatgcg ccatgcgaaa
      181 agaggggact tctcgcatct cttcggaaag aaaatcccat agggaatccg cctgttcttc
      241 ggagagaatc ccttttttcg ctgcacttct caaatcatct ctgccgataa tcatctctca
      301 ctcacctccc gggaaagatt tgccgtcata ttcgtattct acctcataat cggacgcttt
      361 ccagagcttc ttcaaaatac ttcgacggta gatttttttc tccggatgaa atagactggt
      421 tgaagagagc gcatcggggt tctttcatcc gggaaatgcg cacttccggt tcggaccgga
      481 gaaaggagca acgaatgatt gcgaagaaag gttcaaaagt gaaagtccac tatacgggca
      541 ctctgaaaaa cggggatgtc ttcgactcct cagaaggacg atcccctttg gagttcaccg
      601 ttggcgccgg acagatgatc cccgggtttg acaagggggt tgaagggatg aacgcaggag
      661 agaaaaagac catcacactc ccccccaaag aagcctacgg ggagaagaga gacgacatgc
      721 tcttcagagt ctcgcgcgac gtcctcccgg aaggatacac gccctccatc ggcgatcctc
      781 tcagagtagc gacagacgac ggcggcgtac tcaacgtctt tatcgccgag atggacgagg
      841 atggaatcac tctcgacggg aatcaccgcc ttgccggcga agaactcaca tttgaagtaa
      901 caatggtaga aatagcctga gcaacgccac caaataaaag aggaggccgc agcgaggaga
      961 cacgccgcgc ctcctctttt atttcccggg aaatacaact gaaaggagag atcaaatatg
     1021 ccctttcaat tttctgaacc attttcacga agagcacatc tgcttcaaaa cggaagtcaa
     1081 ggcaaaggtc ctgttctcta ttggatgagc cgcgagcagc gcgcagaaga cagctggcct
     1141 ctcctcgccg cggcgcacgc ggctgcttcg cttgaccgcc ctctcatctg ccttttctgt
     1201 ttcgctcctt ccttcgccca cgctcagaag cgacactatg ccttccttat acaaggtcta
     1261 aaagaaacat cccgacaact acgcaaatat aacacgcaaa tgaaaatact atacggagaa
     1321 ccaggcccta ctgtcgctgc actcacggcg acgctcgatg cagcgcttgt cttcatcgat
     1381 ttcgacccgt tcccggagaa gcagccgtgg agaaaggaat tcctcgacag agtttcgaca
     1441 acggtctacg aggtcgacgg gcacannnnn nnnnnatccc ggcgtggatc gtatcaccga
     1501 agaaggagta cagcgccgcg acatttcgca gaaaagtaca ccctctgctt gattcctatc
     1561 tcgaagagta ccctctgttt ccggaactcc ctccgcgaga gagtatagaa acgccgcttt
     1621 ttctctctct tgaagatgct gccgcccgag tcgcaacaga aaccgcagaa gcgccccttt
     1681 cgccttactc gcccggctct tccgctgccc gcgctgccat gcgccggttc ttttcagaaa
     1741 gattgaatga ctacgacact cgaagaaaca atccggcgct ttctgccctc tccgatcttt
     1801 cgccgtggct ccacttcggc cagctctccc cgcaaagagt cgcactggag gcgctgaaaa
     1861 caccccaatc ccccgcgagg gaagcctttc tcgaagaact catagtccgt cgagagctgg
     1921 cagataattt ctgtttttat gagccaatgg ccggaaccta cgatggactc cctgaatggg
     1981 gccggaaaac gctcgcgctt catgcgggcg accggagacc tcgtctttac acaatggcgg
     2041 agcttgaaga agcccgaacc ggcgatcctc tctggaacgc tgcgcaaaaa gagatggtct
     2101 ggaagggaaa gatgcacggg tgggcgagaa tgtactgggc gaagaaaatc ctcgaatgga
     2161 cggagacccc ggaagaggcc tttgaacgtg cgctcattct caacgaccgg tacgagcttg
     2221 acggacgcga ccccagcggc gtcgtaggcg tttcatgggc aatcggcggt cttcacgaca
     2281 gaccttgggc tgaacgctct gttttcggga tggtacgcta tatgaacgac aagggagcgg
     2341 cgagaaagtt tcgtgtagcc gactatatcg agagcgtcaa cgcgtttata tcgaaccgaa
     2401 cgggagagaa tctcagctaa gatcagtatt agtcaacgtc tatgaacact actttgttta
     2461 aaattctgct catcggaagc atgtagatcg tgatattgga gacacgctcc tctttctgca
     2521 acgaagatga gggcggcccc ccccatttca aacataacac aatgccgccg gagaaaggcc
     2581 tgagtcctct caactggcgg cattgtcatt cgttcattga acaccgggat tccggtctgt
     2641 tacggcttgt atcgtgtaag cctcccgtgc agaggcgctc tccgcaactc tgcaccgctt
     2701 gtaccaagaa gcggacgtat tttcaaagca aacagcgcgg ccactccccc cttgagaaga
     2761 tcagggagta agaagggcag aacaccgatc ttcacaacct ggtacacacc gatgctcttt
     2821 ccggccaaaa acttcaggtt gagatagagc actgaagcgc cgagcaaata aataaacgct
     2881 atccccgcac atgaggcaac aacgaaacgg atcgcgttcg ctccttcctt ggaagagatc
     2941 cggccaacga tccatgcggc cgcaggatat gagagaaggt agccaaacgt cggagaaaag
     3001 accgttccga tccctccccc gccgctgaaa accggtagtc ccgcaagtcc gataaacagg
     3061 tagatgacct gtgatagcat gccgtatttc ggtcccagcg taaaaccggc gagaagcacc
     3121 attaatacct gaagcgtcag cggaaccaga gggaaaggaa tacgaagaaa cgttccaaca
     3181 actgtaaggc cggcgaaaaa accggcaaga atccaccctt tgtatctcat cgagcacccc
     3241 ctcagctatg tcacccggat attttagtgg ttgacatagc tttttgcaac gtatttccgc
     3301 ctcctcgtct gcttctctct taaaggaagc catattccgt tgtcagcgaa caggcgaagt
     3361 atatgttctc tgggaaaaat ctttcacgtt cctgtaaaca gtctctctga tttctagtct
     3421 tcgcgagaaa gaaacaccat aattgcgtat gaagaagctg atctatactg acctacaccc
     3481 tccaaatagg tctctttgta tcgaaaggat gttccaacag ttgacaacgt tcaccctcac
     3541 cagtctgaag gcacacttcc ggaatagcgt acaaccgcta atttgcagcg catgcaatca
     3601 cctgtaacaa ttcacaaagt ctccaactac aaagaggccc ccaacgtgcc tctcaaagca
     3661 gcgcactata acaaagatac agagcatttt ccgcgatgtc ttgggtacag ccgatcccgg
     3721 ccgcgcatgc aattgataca atcggccgca tgaaaggata aatgggtttt cggactcttt
     3781 tgtctttcga accacacatt
//
LOCUS       DAOSWW010000145         3792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_16929_length_3792_cov_2.885737, whole genome shotgun sequence.
ACCESSION   DAOSWW010000145 DAOSWW010000000
VERSION     DAOSWW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16929_length_3792_cov_2.885737"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..713)
                     /locus_tag="PK442_04160"
     CDS             complement(195..713)
                     /locus_tag="PK442_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86872.1"
                     /translation="MNKWTKFFTLAVMAGLLCLFGTSAFAELPKGSIAVLTKGPSVQH
                     SAAVASILTRELLAKGHKVVDPKRLDAIRRSKAAALALEGNADAIIKLSKQYGFSTMI
                     TAQLQAGKPVLNEFQLYTGTSSLAIMVMSSGGQMIYADTVMGKQVGYTPDEAAQKSIE
                     AAAKLAVQKMLQ"
     gene            complement(706..1215)
                     /locus_tag="PK442_04165"
     CDS             complement(706..1215)
                     /locus_tag="PK442_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86873.1"
                     /translation="MKKQVRRLRREKAPRPFRFFLVVCVCIATAGALTAAVPALFSGN
                     GDPGAEAQLVGEENPEMPRDPGIPETVNSGLLLREQGIATAEGVGRIPEDTAQGRLLG
                     RRAALTDARRNLLVLRQKLLDGKFDARSVSGKIAEVRIYSEKTDGALYYLKVDVPLYR
                     LMKGDVPVE"
     gene            complement(1187..2356)
                     /locus_tag="PK442_04170"
     CDS             complement(1187..2356)
                     /locus_tag="PK442_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86874.1"
                     /translation="MRIRNFFFVFLMILLSAAPAFSAGVQAAGELIKVEAEGFAPIKG
                     GDKNAAREAAKRSAYRDALEKALGAYVTGITEMQNFEVVKDKVFSQTTGIVKKFDIVR
                     EKVGDDGILYITAICHVAQAALDGVLGPAVLDALGNPRVMVLLDERIGDKRSFLSTAE
                     SETMRVFEKAGYLLVDSTQASILKNIDLDAVRESADPEVFRQLARDFQADVLIYGKAY
                     ASSFANQVVSGVRIYGVRSTVQLKAVLANTAYQLGSDAVEEKTKGVSVEDGAIKGLQP
                     AAMKASGSIVHKIAYTLISGSAGGIPGRTIKVKISNMTFKDAKELKESLQDVQGVTAV
                     YQRLFRSKRLELDVVSDMTAEEIASLLSDLGYDIEDLSSVTVEGVLNEKTGEKTP"
     gene            complement(2360..3190)
                     /locus_tag="PK442_04175"
     CDS             complement(2360..3190)
                     /locus_tag="PK442_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86875.1"
                     /translation="MKRLLACVLMMSIFLPGSISFATEVEIELHPITTEKEKGLIDWT
                     QNHIEATGMAVAPKNMKGAQAKALARRGAIVDLQRNLLEFMGGVQVDARTTMDDFMAE
                     DRVRSEVHGMIKNVELLKGEWDGEAYTVTGRIKLAEVRSIVCPRVPVDTIIIETERKT
                     PAPKASGKYTGLVIDVRHLPLIPSVTFRVFDESGRAVYGVEFANQASMLQSGICSYYN
                     NINYAQGELRVAPNPVKTKAIRLSTGNVDIVIPNSAAAKIRGSSFDFRKECKVLLVVK
                     "
BASE COUNT          771 a         1119 c          986 g          916 t
ORIGIN      
        1 tcatctcggt gatcgccgcc gccggaatac cgcaccccgc cttgttgtcg aacttgcgca
       61 cggaaagcct cggtctctgg gagagcggcg cagcgagcgc agcccccgcg aagatcgaga
      121 ggcagacgat cgcaagaacg cacgcgaaaa gtttcttttt catgagattc tccttcgctc
      181 cggcgcgttc cgcactattg gagcatcttt tgcaccgcga gtttcgcggc ggcctctatt
      241 gatttctgcg cggcttcgtc cggcgtgtat ccgacctgct ttcccatcac tgtgtcggcg
      301 tagatcatct ggccgcccga actcatcacc atgatcgcga gggacgaggt tccggtgtag
      361 agctgaaact cgttcagaac gggctttccc gcctgaagct gggcagtgat catcgtgctg
      421 aaaccgtact gtttgctcaa cttgatgata gcgtccgcat tcccttcgag agccagagcg
      481 gcggccttgc tgcggcggat tgcatcaagg cgtttcggat cgactacctt gtgccccttc
      541 gcgagaagct ccctggtcag tatggaggcg acggcggccg aatgctgaac tgacggcccc
      601 ttcgtcagca cggcgatcga tcctttcgga agctccgcaa atgcgctcgt accgaaaagg
      661 cacagcaaac cggccattac cgcaagcgta aaaaactttg tccatttatt caaccggaac
      721 atcccccttc atcagtctgt acaacggaac gtcgaccttc aggtagtaga gggctccgtc
      781 cgttttctcc gaataaattc gtacttcggc gatcttcccc gaaaccgacc gcgcatcgaa
      841 ctttccatcg agcagttttt gccgtagcac cagcagattt cgcctcgcgt cggtcagcgc
      901 ggcccttcgg ccgagcagcc ttccctgagc cgtatcttcc gggatgcgac cgacgccctc
      961 cgctgtggca atcccctgct cacggaggag aagcccggaa ttcaccgttt ccggtatccc
     1021 cgggtcccgc ggcatctccg gattttcttc cccgaccaac tgcgcctccg cgcccggatc
     1081 cccgtttccc gaaaagagcg ccgggaccgc cgccgtcaac gctccggcag tcgctatgca
     1141 gacgcacacg acgaggaaaa acctgaaagg acgcggcgct ttttctctac ggagtcttct
     1201 cacctgtttt ttcattcagg acgccctcca ctgtaacgga cgacaggtct tcaatatcgt
     1261 atccaaggtc ggagagcaag gaggcgatct cctccgcagt catgtcggac acgacgtcca
     1321 attcgagtcg cttgcttctg aacagcctct gatagacggc agtcaccccc tggacatcct
     1381 ggaggctctc cttgagctct tttgcgtctt tgaacgtcat gttggagatc tttaccttga
     1441 tcgtccttcc cggtatgccg cctgcggagc cggaaatgag cgtgtaggct atcttgtgca
     1501 cgatggagcc gctcgctttc atggcggcgg gctgcaatcc cttgatcgcc ccgtcctcga
     1561 cggagacgcc cttcgtcttc tcctcgactg cgtccgatcc cagttgatat gctgtgttcg
     1621 ccagcaccgc cttcagctgg accgtgcttc gcacaccgta gattctgacc ccgctgacga
     1681 cctggtttgc gaaggaactg gcataggcct tcccgtagat caggacatcc gcctggaagt
     1741 cgcgggccag ttgtcgaaag acctccggat cagcgctttc acgaacggcg tcgaggtcga
     1801 tgttcttcag gatgctcgcc tgcgtcgaat cgacgagcaa atagccggcc ttctcgaaga
     1861 cgcgcatcgt ttcgctttcg gcggtggaga gaaaagatct tttgtcgccg attcgctcgt
     1921 cgagcagcac catcactctc gggttgccga gagcgtcgag cacggcgggc ccgagaaccc
     1981 cgtcaagagc cgcttgcgca acatggcaga tcgccgtgat gtagagtatc ccgtcgtctc
     2041 cgaccttttc cctgacgata tcgaacttct tgacgattcc cgtcgtctgc gaaaaaacct
     2101 tgtccttgac cacctcgaag ttctgcatct cggtgattcc ggtgacgtac gctccaagag
     2161 ccttttcaag cgcatccctg taggcggaac gcttggcggc ttccctcgcg gcgttcttgt
     2221 cgccgccctt tatcggagcg aacccctcgg cttcgacttt gatgagctcc cccgcggcct
     2281 gaactcctgc ggagaacgca ggagcggccg acaagaggat catcagaaaa acaaaaaaga
     2341 aattccgtat acgcatgtcc tatttcacga caaggagcac tttacactct ttgcggaagt
     2401 cgaacgagct gccgcggatc ttcgccgccg ccgagttcgg gatgacgata tcgacgttcc
     2461 cggtcgaaag gcgaatcgct ttggtcttga cgggatttgg agcgacgcgg agttcgccct
     2521 gggcgtagtt gatgttgttg tagtaggagc agattcccga ctggagcatc gaggcttggt
     2581 tcgcgaactc gacgccgtag acggctctgc cgctttcgtc gaagacgcgg aaggtcaccg
     2641 agggaatcag cggaaggtgc cgtacgtcaa tgacgaggcc ggtgtacttt ccggaagcct
     2701 tgggcgcggg cgtctttcgt tcggtctcga taatgattgt atcgacggga accctggggc
     2761 atacgatgga acgcacttcg gcgagtttga tccgcccggt aacggtatag gcctccccgt
     2821 cccactcgcc cttgagaagc tccacattct tgatcatgcc gtgcacttcg gaacggactc
     2881 tgtcctcggc catgaaatcg tccatggtcg tcctcgcgtc gacctgcact ccgcccatga
     2941 actcgagcag attccgctga aggtcgacga tcgcgccgcg tcgggccagt gccttcgcct
     3001 gtgcgccctt catgtttttc ggtgcgaccg ccattcccgt ggcttcgata tggttctgcg
     3061 tccagtcgat aagccctttt tctttttccg tggttatggg gtgcagctca atttccactt
     3121 ctgtcgcaaa agatatgctg ccgggaagaa atatactcat cataagtacg cacgcaagta
     3181 gacgcttcat agactcatcc ccctatcagt tgttcgaaca tgatgaaact actctgtcgc
     3241 agactgtcca acccctcctt ctttagatta aaattaacag acaacatgca gcatcacagg
     3301 attcgagtag aagagaatcc tcttcttttg tgtaccgaag cactattagc gaaaaaaacg
     3361 gattgcaaat tcgagcgaaa aaataaagaa ggcaaacaaa cctgttcgaa cactctgtgc
     3421 attattttct tgataaagat tgtgagggaa acacctaaga aaaacaaggt ttttgtgcat
     3481 tgattttatt tttattcatt ctgttcattg tattttttgt gtatttcact ctgcaaaaaa
     3541 gagccgaaga gagggaaacc tgcaccggct tcccctctct tcggctctta tcttaacaac
     3601 tgtttgcgtt atttgttgcg cgtactatgg ttctggaaat ttactgtctt acggcttcac
     3661 cttcacgaag aacactgcct gcttcttttc aatatctgac ttcaggatga tgccgtcctt
     3721 atccttgggg ttgtggaact cgtccatggt gagcgtcgcg tgcataagct tcagatcctt
     3781 cgtcgcctca ag
//
LOCUS       DAOSWW010000146         3760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17132_length_3760_cov_3.585695, whole genome shotgun sequence.
ACCESSION   DAOSWW010000146 DAOSWW010000000
VERSION     DAOSWW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17132_length_3760_cov_3.585695"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PK442_04180"
     CDS             <1..1393
                     /locus_tag="PK442_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO86876.1"
                     /translation="TPPPLREQFAVFFLRTMKSRLANKSYRVSALLQPLLLALAAGLL
                     FRGSWGGEYVFRNNNNLPGYFFISTVIAVFLGMALSADEINRDRKILERERLLRLSRG
                     AYLASKFAWLLLAAALQTGIYTLLGNGLLHIPSSGFATWAILFSTAYCAAMIGLILSD
                     TLSSSSAIHILIPVLLAPQIMLGGPTIPFDELLRKDAGNRNVPLIAEIMPTRWGYEAL
                     MVEHYRNNPFQKTFFHDETREKWFDFLTGVYIPEVRGLASYPFSPTLDIAGIPADADK
                     AGRRLKILRNELDYLERFTGLQWRGEGLNDGDLVLERYTREVQRKVMARLKEAEAVLK
                     PLREETAQRRVATEDALRGMLGHKGFEEFKNRSFNREVEKQLLGISAEDAIVISGPRL
                     VPKVLPVVMEPESRFGRAHLFAPFKRLGDFKVQTPFFNIGVLWLLSALLAAALRTRLV
                     PRAATLFRRFTGR"
     gene            complement(1444..>3760)
                     /locus_tag="PK442_04185"
     CDS             complement(1444..>3760)
                     /locus_tag="PK442_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHLP bacteriocin export ABC transporter
                     permease/ATPase subunit"
                     /protein_id="HOO86877.1"
                     /translation="QTEELLRRGEFAAVFDAFCEMIFSSLAMNARLRAADDLGALRQM
                     QKSSRKALSSALGTLGEALSGKRRKEAASREESPLRRAFSTVAAFLGRSVAAPKGEEL
                     ASERGESLRDLARASNLQLRRVTLAGPWWISDGAPLIAYVQENAEKSSGGGEMSGTPV
                     ALLPSGRGYRLVDDEGERRVDERIAATLGQDAIQVYRPLPGSPLGGMDLLRFGLAGTK
                     RDMGAVLVISVCLALLGMISPEVNRQIFGEVIPQAERGRMLQLFGILVSVALSSGLLH
                     LANGTAFLRTESVMDHDVSAGIWDRLLRLPVGFFRKTTSGDLANRALGLFMLRETASR
                     TVKTLLVQSVFLIFFLGQSFWYDWRLALSGLACLFVPAALTVVVNVVQLRFQRKMIGL
                     QNRLLALTFQIMTGISKIRVAGAEERSFARWAELFGAQRTLGTSARRLEVFLNTVMAL
                     FPLSVSIVILFALVRWSAADASYETGRFMAFWSAFGALQGAFFQMVSSVTTTLNMLPM
                     VENLAPILQAEPETGDLKTDPGEITGRIDLEGVVFRYLKEGPPVLKGLDLHIEPGEFV
                     AVVGPSGAGKSTLIRLLLGFDQPESGGIFYDNKDIAELDLGKLRRQIGVVLQSGGLLS
                     GDIASNVRCSRNMTVEQIEDALRTAGMEEDIAAMPMGIHTVITDGANTLSGGQKQRLL
                     IARAVAGKPRVLIFDEATSALDNRTQAMISRSLESIQATRIVVAHRLSTVIGADRICV
                     LEDGRLVEEGKYDELMAKDGLFAELVRRQTV"
BASE COUNT          719 a         1241 c         1151 g          649 t
ORIGIN      
        1 cacccctccg ccgctgcgcg agcagttcgc cgtcttcttt ctgcggacga tgaaaagccg
       61 tctcgcaaac aagagctacc gcgtttcggc gctcctccag ccgctgctgc tcgcactggc
      121 cgcaggcctc ctcttcagag gttcatgggg gggcgagtat gtcttcagga acaacaacaa
      181 ccttccggga tatttcttca tcagcacggt gatcgccgtc tttctcggga tggccctcag
      241 cgcggacgag atcaacaggg acaggaagat cctcgaacgc gagcgcctgc tccgattgag
      301 caggggcgcg tatctcgcct ccaagttcgc gtggctcctc ctcgcggcgg cgctccaaac
      361 aggaatctac acgcttctgg gcaacggctt gctccacatc ccctcgtcgg ggttcgccac
      421 gtgggctatc ctcttcagca cggcatattg cgcggccatg atcgggctga tcctgtcgga
      481 cacgctgtcc tcgtcctcgg ccattcacat tctcatcccc gtgctgctgg cgccgcagat
      541 catgctcgga gggccgacga tccccttcga cgagctgctc cgcaaggacg ccggaaaccg
      601 aaacgttccc ctgatcgcgg agatcatgcc gacgcggtgg gggtacgagg cgctcatggt
      661 ggagcactac aggaacaacc cgttccaaaa gacgtttttt cacgacgaga cgcgggagaa
      721 gtggttcgat ttcctcacag gggtgtacat ccccgaggta cgcggcctgg cgagctaccc
      781 gttctctccg acgctcgaca tagccgggat ccccgcggac gccgacaaag cgggccgacg
      841 gctgaaaatt ctgcggaacg agctcgatta tctggagcgc tttacgggcc tgcaatggcg
      901 gggcgaggga ctgaacgacg gcgatctcgt tttggagagg tacactcgcg aggtccaacg
      961 gaaggtgatg gcgcggctca aagaggcgga agcagttctc aaacccctcc gcgaagagac
     1021 cgcgcaacgc cgggtcgcga ccgaagacgc gcttcgcgga atgctcggac acaaggggtt
     1081 cgaagagttc aagaacagga gtttcaacag agaggtcgaa aaacagctcc tcggcatctc
     1141 cgccgaggac gcgatcgtca tatccgggcc gcgcctcgtc ccgaaagtcc tccccgtcgt
     1201 catggagccc gagtcgcgct tcggcagggc gcaccttttc gctccgttca aacggctcgg
     1261 agatttcaag gtacaaacgc cgtttttcaa tatcggcgtc ctctggctcc tctccgcact
     1321 tctggccgcg gctctgcgga cgcgcctcgt tccgcgcgcg gcaacgctct tccggagatt
     1381 cacagggaga taacgccacg tccccaaaaa cgagccgcag gaatgccttc agtccgtttt
     1441 cttctacacc gtctgtcttc ggaccagttc ggcgaagaga ccgtccttcg ccatcagttc
     1501 gtcgtacttc ccctcctcga caagacgccc gtcctcgagg acgcagatac ggtcggcgcc
     1561 gatgaccgtg gagagccggt gcgccacgac gatgcgcgtc gcctggatgc tctccaagct
     1621 gcggctgatc atcgcctgcg tccggttgtc cagcgcgctc gtggcctcgt cgaagatgag
     1681 gacgcgcggc ttcccggcga cggcgcgggc gatcaggagg cgctgcttct gccctccgga
     1741 gagtgtgttc gcgccgtccg tgatgaccgt atgaatcccc atcggcatcg cggcgatatc
     1801 ctcctccatc cccgccgtcc gcagcgcatc ttctatctgc tcgaccgtca tgttgcgcga
     1861 gcagcggacg ttggaggcga tatccccgga gagcagcccg ccgctctgga gaacgacgcc
     1921 gatctgccgc cgcagcttcc ccaggtccag ctcggcgata tccttgttgt cgtagaagat
     1981 gccccccgac tccggttgat cgaacccgag cagcagacgg atgagggtgg acttgccggc
     2041 cccggatggg cccaccacgg cgacgaattc ccccggttcg atgtggaggt cgagcccctt
     2101 cagcacggga ggcccttcct tcaggtagcg gaagaccacc ccttccaggt caatccgtcc
     2161 cgtgatctct ccgggatcgg tcttcagatc gccggtctcg ggctcggcct ggaggatggg
     2221 ggcgaggttc tccaccatcg ggagcatgtt caacgtagtg gtcaccgaac ttaccatctg
     2281 gaagaaagcg ccctgcagcg cgccgaaggc ggaccagaag gccatgaaac gccccgtttc
     2341 gtacgaagcg tccgcggcgc tccagcggac aagggcaaag agaataacaa tggagacaga
     2401 gagaggaaag agcgccataa ccgtgttcaa aaagacctcc aggcgtctcg ccgaagtccc
     2461 cagcgtccgc tgcgccccga acagctccgc ccatcgtgcg aaggagcgct cttcggcgcc
     2521 cgccacccgg atcttcgata tgccggtcat gatctggaag gtcagcgcaa gaagcctgtt
     2581 ctgcagcccg atcatcttgc gctggaagcg aagctgcacg acgttcacca ccacggtcag
     2641 cgcggccggg acgaacaggc acgccaggcc ggagagggcg agcctccagt cgtaccagaa
     2701 cgactgcccc agaaagaaga tcaggaaaac gctctggacg agcagcgtct tgacggttct
     2761 cgacgcggtc tcgcggagca tgaagagacc aagcgcccgg ttcgcgagat cgccggacgt
     2821 cgtcttccgg aagaagccca ccggcaggcg aagcaagcgg tcccagattc ccgccgaaac
     2881 gtcgtggtcc atcacgctct ccgtgcggag aaaggccgtt ccgttcgcca ggtggagcag
     2941 gcccgacgaa agcgcgacgg agacgagaat accgaagagc tggagcatcc ttccccgttc
     3001 ggcctgcgga atgacttcgc cgaagatctg gcggttcacc tcaggggaga tcatccccag
     3061 aagggccagg cagaccgaga tgacgaggac ggcccccatg tctctcttcg tgccggcaag
     3121 gccgaaacgg agcagatcca tgccccccag cggcgagccg gggagcggac ggtagacctg
     3181 gattgcatcc tgcccgagag tcgcggcgat tcgctcgtcg acacgacgct ctccctcgtc
     3241 gtcgacaagg cggtatccgc gtccggacgg aaggagcgcg acgggcgtcc cgctcatctc
     3301 tccccctccg gaagactttt ccgcattctc ctgtacgtag gcgatcagcg gcgcaccgtc
     3361 cgagatccac cagggaccgg cgagcgtcac gcgacggagc tggaggttgg aagcgcgcgc
     3421 gaggtcgcga agcgactccc cgcgttcgga ggcgagttcc tctcctttcg gagcggcgac
     3481 gctccgcccc aaaaacgcgg cgaccgtgga aaaggccctc cggagcggcg actcctcacg
     3541 cgaggctgcc tctttccgcc gttttcccga aagcgcctcg cccagcgttc caagagcgga
     3601 ggaaagagct ttcctgcttg atttctgcat ctgccggagc gccccaaggt cgtcggcggc
     3661 ccggagccgg gcgttcatcg ccagcgagga gaagatcatc tcgcagaacg cgtcgaagac
     3721 ggcggcgaac tcgccccttc ggagcagctc ttccgtctgg
//
LOCUS       DAOSWW010000147         3755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17173_length_3755_cov_6.288919, whole genome shotgun sequence.
ACCESSION   DAOSWW010000147 DAOSWW010000000
VERSION     DAOSWW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17173_length_3755_cov_6.288919"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /gene="cas6"
                     /locus_tag="PK442_04190"
     CDS             complement(<1..730)
                     /gene="cas6"
                     /locus_tag="PK442_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOO86878.1"
                     /translation="MRIAVTFSIPFGSSIPVNYNHILTGLVYDLAGKEAPEWAARLHE
                     EGFQGTGRRPYKFFTFSQLFGGPGSTKVENGRLVFTTDTLRWTFASCLDVMATIFMDA
                     LLSSGTVEIGGMKAKVASVTSEPPRSFESGKGRFTCISPLVGSVYDSQKAHKYLSPND
                     PSFWKVISINISRKWAALTGEETEGEVRFEPDREYMERKRTSKAITIKPGEVVIGHML
                     PFTAFGPSELLALGYESGFGSRNSL"
     gene            1147..2562
                     /locus_tag="PK442_04195"
     CDS             1147..2562
                     /locus_tag="PK442_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO86879.1"
                     /translation="MNPILQQLPSDDDTLSSSAAEFFHTFDVGRLFTRSARSKKGVGA
                     FKILQFLFALVFLKKSFFDMMQQKSLPWSKDTFYRFLSESRINWRRFLLLVATRIIDR
                     FLHHLTEEKKDRVLVLDDSTYSRDRSKKVEMLSKCYDHCDHKYFKGFRMLTLGWSDGR
                     SFLPLAFSLLGSAKEENRVFEAGGNDGRTSGGRRRKEAVMKTPEASLALLDSALANGV
                     KADFLLFDSWFATNRFLHSVRTRRIHVIAMLKDFNSMQFLVKREDGTKKRMKLGGLYE
                     KVSAGFGKKNILGSALVEVDNGKDSSETPLSVKVVFVRNRKSDAKREWLAILCTDASV
                     PDDEIVRLYGKRWSIEVFFKTCKSMLRLAKEFQTRSYDSLVAHTSIVFLRYMMLCFEQ
                     RRAKDDRACGELFRCCCEELEDISFVASLALLLETLKTAVRSGVAFTEETLEKLFGEF
                     VKAMPAFLRKSLGVSLAFSLS"
     gene            2917..>3755
                     /locus_tag="PK442_04200"
     CDS             2917..>3755
                     /locus_tag="PK442_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HOO86880.1"
                     /translation="MEHGRPLRSGVAPRGKEYKAEAEISDTDRRGVGGVHSIDDGADN
                     TTRPREGTLLHPRLHERTGDLLHELSGSEEKARAFQRNLYRKAKREKEFRFYSLYDKI
                     WRRDILEYAWKRCRANGGSPGIDGKSFKAIEEEGVGRLLEEIARELREKTYRPSPVKR
                     VYIPKADGSKRPLGIPTIKDRVVQTACLIVVEPIFEADFEDISYGFRPKRSAHGAIEE
                     IVEHVERGFTAAYDADLTKCFDTIPHDVIMEGLKRRVSDGNVLSLVLKFLRTPVVEPD
                     GPV"
BASE COUNT          856 a         1032 c         1103 g          764 t
ORIGIN      
        1 ccaggctgtt ccggctgccg aatccggatt cgtagccgag ggcgagcagt tcggacggtc
       61 cgaacgccgt gaaaggaagc atgtgtccta tgacaacctc tcccggtttt atcgtgatcg
      121 ccttgctcgt tctttttcgt tccatgtact ctctgtcggg ttcgaagcgt acttctcctt
      181 ccgtttcctc tccggtaaga gcggcccatt ttctagaaat gtttatagag ataaccttcc
      241 aaaaagatgg atcgttcggc gaaaggtact tgtgcgcctt ctgggaatcg taaacgctgc
      301 cgacaagagg ggagatgcac gtgaaacgtc ccttcccgct ctcgaacgat cggggcggtt
      361 cgcttgtgac ggaggcaacc ttcgccttca tgccgccgat ctccaccgtg ccggatgaga
      421 gaagggcatc catgaagatg gtcgccatca cgtccaggca ggaagcaaat gtccagcgga
      481 gcgtgtcggt tgtgaagacg aggcgaccgt tctccacctt cgtcgagccc ggaccgccga
      541 aaagctgact gaacgtgaag aacttgtacg gtctccgtcc cgttccctga aatccctcct
      601 cgtgcagacg ggcggcccat tccggcgcct ctttcccggc aaggtcgtag acgagaccgg
      661 tgagaatatg gttgtagttg acaggaatgc tcgatccgaa cgggatagag aacgtcactg
      721 ctatccgcat gaagaattcc tcctctgatg aatggttggt ctctcaacct caatgaaaac
      781 atgtcgtttc caagatgaca gactcagcgt gtttcccgtg gggtacacag ttgcgatagc
      841 attaaaacga ttcggcaggc agctttagat agctcattgt ttcctttttc cgattccggc
      901 ggcggatttt gccgcgaagg cgctttaccc gtcgcctggc ggatgtcttc gatgttgtgt
      961 atgtccagga atcaatcttt ccttcgggct tcataaggaa gcgccccctt tcttttatac
     1021 tcaactttcg caggaccttg aaaaccgaaa accgttgtca tgactgaaaa aaaatgacag
     1081 gaaggcctct gttttgtaaa attgtagtcg cgacacaaac aaaactacaa gacagggggc
     1141 cttcccgtga atcccattct acagcaactt ccgtccgacg acgataccct ttcttcttcc
     1201 gccgccgaat ttttccatac cttcgacgtc ggcaggctct tcacccgttc ggccaggtcg
     1261 aaaaaaggcg tcggggcgtt caagattctc cagttcctct tcgccctcgt cttcctgaag
     1321 aagagcttct tcgacatgat gcagcagaag agtctgccct ggtccaagga tacgttctac
     1381 cgctttctct ccgaatccag gatcaactgg agacgctttc tcctgctcgt agcgacgcgg
     1441 atcatcgaca gattccttca ccatctcacg gaggagaaga aagaccgggt gctcgtcctc
     1501 gacgactcca cctactccag ggacagaagc aagaaggtgg agatgctctc gaagtgctac
     1561 gaccactgcg atcataagta cttcaagggg ttccgcatgc tcaccctcgg ctggtcggac
     1621 gggagatcgt ttcttcctct cgccttctct cttctcggct ccgcaaagga agagaaccga
     1681 gtcttcgaag cgggcggcaa cgacggacgg acttcggggg gaagacggcg gaaagaggcg
     1741 gtgatgaaga cgccggaagc atctctcgct cttctcgatt ccgcgctcgc caacggcgtg
     1801 aaggccgact ttctcctctt cgactcctgg ttcgcgacga accggtttct ccactccgtg
     1861 cgcacgaggc gcatccacgt catcgccatg ctcaaggact tcaacagcat gcagtttctg
     1921 gtgaagcggg aggacggcac gaagaagcgg atgaagctcg gaggtctgta cgagaaggtc
     1981 tccgccggct tcgggaagaa gaacattctc ggctccgcgc tcgtcgaagt ggacaacggg
     2041 aaggactctt cggaaactcc cctgtccgtg aaagtcgtgt tcgtcaggaa caggaagagc
     2101 gacgcgaagc gggagtggct ggcgatcctc tgcaccgacg cgtccgttcc cgacgacgag
     2161 atcgtgcggc tctacgggaa gcgctggtcg atagaggtct tcttcaagac atgcaaaagc
     2221 atgctccggc ttgcgaagga gtttcagaca cgcagctacg actctctcgt ggcgcacacg
     2281 agcatcgtct tcttgagata catgatgctc tgcttcgagc agcggcgtgc gaaggacgac
     2341 cgcgcctgcg gagagctttt ccgctgctgt tgtgaggagc tggaggacat atcgttcgtc
     2401 gcgtcgctcg cgttgcttct ggagacgctg aagacggccg tccgctcggg ggtggcgttc
     2461 acggaggaga cgctggagaa gctattcggg gaattcgtga aagcaatgcc ggccttcctg
     2521 aggaaaagtc tgggcgtttc tttggctttt tccctgtcat gacaaccatt ttcggttttc
     2581 aaggtcctgc gaaagttgag gtgcgccgtg aaaagtcata tgcgaaccag aagggtgcga
     2641 gtcccttccg cggaatatcc cgttcaccgt ggaaccgccg gttggcgtgc aggggcgacc
     2701 cgaagcgtca agcaagccgg aaaaggctct tgaacgagtc gggtgaagat gcgggccgca
     2761 gcgcaagcga acccgtggtg gcctcgaaaa cgcagttgtc gtcggcgatc ccgcctcaac
     2821 agggagaagc ctgacaccga tgtacgaagc aacgggaaga agcagacatc gggacggccg
     2881 tggtgattag gggcggcacg catcgaagga gcgcgtatgg aacacgggag acctctccgg
     2941 tccggggttg cgccccgagg gaaggaatat aaggcggagg ccgaaatttc cgataccgac
     3001 cggagaggag tcggaggcgt ccatagtatc gacgacggcg cggacaacac aacccgcccg
     3061 agagaaggga cgctgcttca tccacgattg catgaaagga cgggagatct attgcacgag
     3121 ctatcaggat cggaagaaaa ggcccgcgcc ttccagcgca acctgtaccg aaaagccaag
     3181 cgggaaaagg agtttcgatt ctacagcctg tacgacaaga tatggagacg cgacatactg
     3241 gaatacgcct ggaaacgatg cagagcgaac ggaggaagcc cgggaatcga cgggaagagc
     3301 ttcaaggcca tcgaggagga gggtgtgggg cgactcctcg aagagatagc gcgcgaactc
     3361 cgggaaaaga cctatcgccc ctcgccggtg aagagagtgt acataccgaa ggcggacgga
     3421 agcaaacggc cgctggggat accgacgatc aaggacagag tggtgcagac ggcgtgcctc
     3481 atagtcgtcg agccgatctt cgaagcggac ttcgaagaca tttcctacgg attccgcccc
     3541 aagaggagcg ctcacggagc catagaggag atagtcgaac acgtggagcg gggattcacg
     3601 gccgcctacg acgcggacct gacgaaatgc ttcgacacca tcccgcacga cgtcatcatg
     3661 gaaggcctga aaagacgcgt atcggacggc aacgtgctct ctctcgtcct gaaatttctc
     3721 aggacacccg tggtggaacc cgacggaccg gttca
//
LOCUS       DAOSWW010000148         3749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17209_length_3749_cov_3.560097, whole genome shotgun sequence.
ACCESSION   DAOSWW010000148 DAOSWW010000000
VERSION     DAOSWW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17209_length_3749_cov_3.560097"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK442_04205"
     CDS             complement(<1..313)
                     /locus_tag="PK442_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOO86881.1"
                     /translation="MVKLRNVSIGKRLGFGFGTVVLIALALGITGILGISRLSNDLEY
                     ITTNRISDLRSLAGLNYQRMAIRAETLGVVITEDKSNRDEELLKILERRKKASDRIDE
                     HW"
     gene            complement(332..877)
                     /locus_tag="PK442_04210"
     CDS             complement(332..877)
                     /locus_tag="PK442_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO86882.1"
                     /translation="MEISADALDQERMDAVLRKRAKKLSREFLKESADEEDLLEIVEF
                     RLGQERYGVASLHVGEVIPLRERAVLPCTPPFVAGILNLRGRIVSLIDLKFFFDIPAT
                     EISKETRVIVLRSGEMELGILADALVGIRMISLRNIHPPLPTMAGVRSAYLLGVEGED
                     TALLDGGRILKDESLIVNEEV"
     gene            complement(896..2368)
                     /locus_tag="PK442_04215"
     CDS             complement(896..2368)
                     /locus_tag="PK442_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOO86883.1"
                     /translation="MTQSSRFILSPEAEQDLRRLGLLVEKRLGLVFSEKRSRDLWQAM
                     LSLAALAQRSPTELMHKWANEGLSEAATTALAEYLTIGETYFFREPKTLRAFEELVLK
                     RFTSGKKPERFRIWCAGCSTGEEAYTLSIILHRLLPDIRSMDVSILGTDINPRVLEKA
                     RRGAYSSWSFRGLEKSLKERFFEPLDDGSWSVKHLFRQRVSFSLLNLADDEWRLWDDG
                     SHPDVLFCRNVLIYFSADQRKNVVFRFHSLLPPEGWLVVAPCESSALLASKFTPVHHD
                     GATLYKKQAVPEPTERDVSFPEPLFSALPVGAMLDEAPMEEEPAETGVPYEEPPFLAA
                     ETFLGTVPEYQEDEEPGEALSESVGACLQNARILADKGRHDEALQWALKAKDLDRTAP
                     EPLYLAALISSETGDEREAAASLRSALFLAPDFVMAHYALASLAMKSGKDADSERHLR
                     NAEALLSSLPPDAPLREGDGLCAGDLLQTVRGLRRNSIFE"
     gene            complement(2365..2874)
                     /locus_tag="PK442_04220"
     CDS             complement(2365..2874)
                     /locus_tag="PK442_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO86884.1"
                     /translation="MTEKKEAFYLIFRVGERRFAIAAAEIVRVLAAVEVTPLPDTPSF
                     LRGVINCGSVLVPVIDLRVRFGLPVRRVRPSDRLIFVRNAASAFQKDGRLCSFLVEDV
                     EGVFPLAPETIALPESSSGLFNAALAGLESGDDRVVCIQTADTLFSMTEAEILQVERA
                     SPGTEDILL"
     gene            3088..>3749
                     /locus_tag="PK442_04225"
     CDS             3088..>3749
                     /locus_tag="PK442_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO86885.1"
                     /translation="MKKILFALFALLVLGTAGWGNVMEKDALLVGTESTYPPYEFRDA
                     NNQLKGFDIEMMETIAAKIGKKIEWVDMPFDSLIPSLLAKKIDIVAAGMSATEERAKR
                     VAFSTNYEISISAFIVAEGNDSMKVLEDMKGKTIAVQLGTVQETYSQSLEGVVVKSFQ
                     KFDDCVREVILGRVDATLMDTPVAKSYVEHKDFQGKIKIAFEQEITGSGKALAMNLGE
                     TEL"
BASE COUNT          912 a         1054 c          986 g          797 t
ORIGIN      
        1 tccagtgctc gtcgatccga tccgaggctt ttttccgccg ttccagtatt ttcagcaact
       61 cttcatcccg gttcgatttg tcttcggtta ttacaacgcc cagagtctcc gctctgatgg
      121 ccatccgctg atagttcaac ccggcaagcg aacgcaaatc ggagatgcgg ttcgtcgtta
      181 tgtattcaag atcgttcgag agccttgaaa tacccagtat accggtaatc ccaagggcca
      241 acgcaatcaa tacgaccgtg ccgaaaccga aaccgagtct cttgccaatg cttacattcc
      301 gtaatttcac catgtcgtcc tcctcactgc tttaaacttc ctcgttgacg atcagcgatt
      361 catccttgag tattcttcct ccgtcaagca gagcggtgtc ctctccctcc actccgagca
      421 gatatgcaga gcgcacgccc gccatcgttg gcagcggcgg atggatattc cggagagaga
      481 tcatgcgtat cccgaccaaa gcgtcggcca gaattcccag ctccatctct cccgaacgca
      541 gcactataac cctcgtttct tttgagatct ccgtcgccgg gatgtcgaaa aaaaacttca
      601 gatcaattaa agaaacgatg cgtccccgga gattgagtat tcccgccaca aaagggggag
      661 tgcatggtag aaccgcacgt tcacgcaacg ggataacttc accgacgtgc agcgaagcga
      721 ctccgtacct ctcctgccct agacggaatt cgacgatttc caaaagatcc tcttcatccg
      781 cgctctcctt caagaattcc ctggaaagct ttttggctct tttccggagg acggcgtcca
      841 tccgttcttg gtcaagcgca tcggcgctta tttccattct ccgtctcacc accctctatt
      901 caaaaatgga gtttcgccgc agcccgcgaa cggtctgcag gaggtctcct gcacagagtc
      961 cgtcgccttc ccgcagcgga gcgtccggcg gaagagaaga gagaagcgcc tccgcgttcc
     1021 taagatgacg ttccgaatcg gcatccttgc cgcttttcat cgcaaggctg gcgagagcat
     1081 agtgcgccat gacgaaatca ggagcaagaa agagggcgct ccggagcgat gccgcagcct
     1141 ctctttcgtc tcccgtctcg gagctgatca gggcagccag ataaagcggt tcaggggctg
     1201 tccggtccag atctttggct ttcagcgccc attggagcgc ctcgtcatgt cttcccttgt
     1261 cggcgagaat ccgggcgttt tgcaggcatg cgccgacgga ttcggaaagc gcttccccgg
     1321 gttcttcatc ctcctgatac tccggaacag ttccaagaaa ggtttcagcg gcaaggaacg
     1381 gaggttcttc gtaaggaacc cccgtctctg cgggttcctc ctccatcgga gcctcgtcga
     1441 gcatcgctcc tacaggaaga gccgaaaaaa gaggttccgg gaaagagacg tctctttccg
     1501 tcggctccgg gacagcctgt tttttgtaaa gggtcgcccc gtcatgatgc acaggggtaa
     1561 acttcgacgc aagtaaagcc gaggattcgc acggggcaac cacgagccag ccttcggggg
     1621 gaagaagcga gtggaaacga aagacgacat tcttgcgctg atcggcggaa aaatagatca
     1681 gcacattccg gcagaagaga acatcggggt gcgagccgtc gtcccagagt cgccattcgt
     1741 cgtcggctag attgaggagg gaaaaagaaa ctcgttggcg aaagagatgt tttacagacc
     1801 agctcccgtc gtcaagaggc tcgaaaaacc tctctttcag agacttttca agccctctga
     1861 aggaccagga agagtacgct ccccgccggg ccttttcgag gactcgagga ttgatatcgg
     1921 tccccaggat cgaaacgtcc attgagcgaa tatccggaag caatcggtgc aaaataatag
     1981 agagcgtata cgcctcttct cccgtactgc atccggcgca ccatatcctg aaacgctccg
     2041 gtttcttccc ggatgtgaaa cgcttcagaa ccagttcttc aaatgcgcgc agtgttttcg
     2101 gctcccgaaa gaaataggtc tctccgatcg taagatactc agccagcgcc gtagtggcgg
     2161 cctctgaaag cccctcgttc gcccacttgt gcatgagctc ggtcggagac cgctgtgcga
     2221 gggcggcaag ggagagcatt gcctgccaga gatcacgaga tcgtttttca gaaaatacga
     2281 gcccaagacg cttttctacc aaaagaccga ggcgccgaag atcctgctcc gcctccggag
     2341 aaagaataaa acggctcgac tgcgtcataa aaggatgtcc tccgtaccgg gggaggccct
     2401 ctcgacttgc agaatctccg cttcggtcat agaaaagaga gtatctgccg tctgaatgca
     2461 cacgacgcgg tcatcgccgc tttcgagccc ggcaagcgcc gcattgaaaa gaccgctgga
     2521 ggattcgggc agagcgatcg tttcaggcgc gagagggaac actccttcca catcttccac
     2581 gagaaacgag caaaggcgcc catccttttg aaatgcggaa gctgcgttcc ggacaaagat
     2641 gagacggtcg ctcgggcgca ctcttctgac aggaagcccg aaacgcaccc ggagatcaat
     2701 cacgggaaca agtacgctac cgcagttaat cacaccgcga aggaacgacg gagtatccgg
     2761 caagggtgtg acttccactg ccgcaagcac tcgcacgatc tccgccgccg caatggcaaa
     2821 ccgtctttct cctactctga aaataagata gaaagcctct tttttctccg tcatctcttt
     2881 ctcccagcct tcccttgatc cgaatacctg ctcgtcctcc aggcgactgg agaaagaact
     2941 cctgtccgga gaaattatag cagtctttgg gaaaaggagg aaagcggtct tgacagggaa
     3001 aaacgtggcg tgtatgctcg caagtacaat gtttatcaca cactgggaaa gtacttaaca
     3061 tctgttcata ttggaggaga gaaaacgatg aagaaaattc tgttcgcact gtttgcgctg
     3121 ctggttcttg gtaccgccgg atggggcaat gtcatggaaa aggacgcttt gctcgtcggc
     3181 acggagagca catacccccc ttatgagttc cgtgatgcaa acaatcagct caaaggcttc
     3241 gatatcgaaa tgatggagac gatcgccgca aaaataggga agaaaatcga gtgggtcgat
     3301 atgccgttcg acagcctcat tccttcgctt ctcgcgaaga agatcgacat cgtcgccgcc
     3361 ggaatgagcg ccaccgagga gcgggcgaaa agggtcgcgt tctccacgaa ctacgagatc
     3421 tcgatcagcg cgttcatcgt ggccgagggc aacgattcga tgaaagtcct cgaagacatg
     3481 aagggaaaga cgatcgccgt ccagcttggg acggtacagg agacctacag tcagtctctt
     3541 gaaggcgttg ttgtaaaatc cttccagaag ttcgacgatt gcgttcgcga agtcattctg
     3601 ggacgcgtcg acgcaacatt gatggatact cccgtcgcga agagctacgt cgagcacaag
     3661 gacttccagg gaaagatcaa gatcgccttc gagcaggaaa tcaccggctc agggaaagct
     3721 cttgccatga atctcggcga aacggaact
//
LOCUS       DAOSWW010000149         3748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17219_length_3748_cov_3.285946, whole genome shotgun sequence.
ACCESSION   DAOSWW010000149 DAOSWW010000000
VERSION     DAOSWW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17219_length_3748_cov_3.285946"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PK442_04230"
     CDS             complement(<1..1370)
                     /locus_tag="PK442_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO86886.1"
                     /translation="MTFGLILITLIVIIIFTTVAALFARYKKCPSDKILVVYGKVAKV
                     DGQHQSSKCIHGGAAFIWPVIQAYHFLDLTPLSIEVNLRNALSKQNIRVDVPSRFTVG
                     ISTEPGIMQNAAERLLGLQLAEIQELSKDIIFGQLRLVIATMDIEEINTDRDKFLANV
                     TANVEAELKKIGLRLINVNVTDINDESGYIQALGKEAAAKAVNDAKKSVAEQDRDGSI
                     GEANAQRDQRIQVAQANAASVEGENLSKITIAQSEAERREKQAESNRLAEAAEKVQAA
                     KALEESYKAEEEAELARARREKATQEADVLVHAGIEKERIQIRADADAEEMRRTAAGE
                     ADAIFAKMSAEARGLREILVKQAEGFERLVKAAGGDTDKAIMMMIADKLPELVKTQVE
                     AIKGIKIDKVVVWDSAGGTSDKTSTANFLSGLMKSIPPLQDMFKMAGMELPSYMAGGQ
                     KNQAPWP"
     gene            complement(1402..2010)
                     /locus_tag="PK442_04235"
     CDS             complement(1402..2010)
                     /locus_tag="PK442_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOO86887.1"
                     /translation="MGEWWSGMLMFEKVFWYIAIPFTALFFIQLVLTFTGMGGEDADG
                     GMEGDAGGEDFDVEGDGDGDIGEAGGFQIFTVKNFITFFTVFGWSGITLHNAGANEYL
                     VLLGSLLIAVAVMLLVALLFVSITRLAQSGTMDVKNALGRTGEVYLPIPAGRAGRGKI
                     SITLQGALREVDAVTAGEALPRGTSVRVVDVAGNGVLLVEKA"
     gene            complement(2053..3486)
                     /locus_tag="PK442_04240"
     CDS             complement(2053..3486)
                     /locus_tag="PK442_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOO86888.1"
                     /translation="MKSIQILCTFLFLFVFLSALPALGAEVVSSTEADQTGVEVTVYN
                     TDLALVKDVRSILLKEGQGELRFMDVASSIRPETVHVKSLNNPPSLAILEQNYEYDLI
                     SPEKLLEKFVGRTVKIEVWNDYQGKKEIVEGTLLSTNNGLVFDIGGQIYLNYPGLKIV
                     PSIPDNLYARPTLAWLYANSAKEKHSVEVSYLTSGMTWKADYIFVLDDADTAADLTGW
                     VTLNNRSGAAYRDATLKLIAGEVQTVADVLPPPMMRMAMGKGVPEAAPAFEEEAFFEY
                     HIYNLARKTTIGNNQTKQISLLEAFGTKIGKEYITRGERRYFTSSLQRGLLKQKVDVV
                     ISFKNSEENNLGMPLPKGIIRMYKKDSKGSLQFIGEDRIDHTPRNEEVRLKVGEAFDV
                     VAERRQTRYKKLADNLYETSWELIVRNRKKDESVVVSFNEPVWGDWEVIASTHPYEQT
                     DAFTLRFDVPAKPDEEVVVKFSLRIRS"
     gene            complement(3521..>3748)
                     /locus_tag="PK442_04245"
     CDS             complement(3521..>3748)
                     /locus_tag="PK442_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86889.1"
                     /translation="LFRLYSTQIALGGLSVMFGSSMLAPGIIPGLLIGVILAANGGVL
                     LYLMRLLLLLDALAESAGGTQPESDEQSVCS"
BASE COUNT          614 a         1170 c         1149 g          815 t
ORIGIN      
        1 ggccagggag cttggttctt ctgtccgccc gccatgtagg agggcagctc catccccgcc
       61 attttgaaca tgtcctggag cggggggatg gacttcatca ggccggagag gaagttcgcg
      121 gtggatgtct tgtcgctcgt tcctccggcg ctgtcccaga cgacgacctt gtcgatcttt
      181 atccccttga tggcctcgac ctgggtcttc accagttcgg gaagcttgtc ggcgatcatc
      241 atcatgatag ccttgtcggt gtcgccgccc gcggctttga ccagccgctc gaagccctcc
      301 gcctgcttga cgaggatctc gcggagtccc ctggcctcgg cgctcatctt cgcgaagatg
      361 gcgtcggcct cgcccgcggc cgtccggcgc atctcctcgg cgtcggcgtc ggcccgaatc
      421 tggattctct ccttctcgat tccggcgtgc accagcacgt cggcctcctg agtggccttc
      481 tcgcgcctgg cgcgggcaag ttcggcctcc tcctccgcct tgtacgactc ttccagcgcc
      541 ttggccgcct gcaccttctc cgcagcttcc gcgagcctgt tggactcggc ctgcttctct
      601 cggcgctcgg cctccgactg cgcgatggtg atcttcgaca ggttctcccc ctcgactgag
      661 gcggcgttcg cctgggcgac ctggatccgc tggtccctct gcgcgttggc ctcgccgatg
      721 gagccgtcgc ggtcctgttc ggcgacgctc ttcttcgcgt cgttgacggc cttggccgcc
      781 gcctccttgc caagcgcctg gatgtagccg gattcgtcgt tgatgtcggt gacgttgacg
      841 ttgatcaggc gcagaccgat cttcttcagt tcggcctcca cgttggcggt gacgttggcg
      901 aggaacttgt cgcggtcggt gttgatctcc tcgatgtcca tggtggcgat gacgaggcgg
      961 agctggccga agatgatgtc cttggacagc tcctgtatct ccgcgagctg gagacccaga
     1021 aggcgctcgg cggcgttctg catgatgccc ggctcggtgg aaatgccgac tgtgaagcgc
     1081 gaggggacgt cgacgcggat gttctgcttg ctgagcgcgt ttctgaggtt gacctcgatg
     1141 gagaggggcg tcaggtcgag aaagtggtag gcctggatga cgggccagat gaacgccgcg
     1201 cctccgtgga tgcacttgga ggactgatgc tgcccgtcga ccttggcgac ctttccgtag
     1261 accacgagga tcttgtcgga ggggcacttc ttgtagcgcg cgaagagcgc cgctactgtg
     1321 gtgaagatga tgatgactat cagggtgatc agaatcagac cgaacgtcat gcgaatcctc
     1381 tcctttcaga tgttgggtgt attacgcttt ctcgaccagc agcacgccgt tgccggcgac
     1441 atccacgacc cgcaccgagg ttcccctggg gagcgcctcc cccgccgtca cggcgtcgac
     1501 ctcccggagc gcgccctgca gcgtgatgga gatttttcca cggccggcac gtccggcggg
     1561 gatgggaagg tagacttcgc ccgttctgcc gagcgcgttc ttgacgtcca tcgtcccgct
     1621 ctgcgccagc cgcgtgatgg agacgaagag cagcgcgacg agcagcatga ccgcgacggc
     1681 gatgagcagc gatcccagga ggacgagata ttcgttcgct ccggcgttgt ggagcgtgat
     1741 gccgctccag ccgaagacgg tgaagaaggt gatgaagttc ttcacagtga agatctggaa
     1801 acctccggcc tcgccgatat ccccgtcgcc gtcaccctcg acgtcgaagt cctctcctcc
     1861 tgcgtcgcct tccattccgc cgtccgcgtc ctccccgccc atgcccgtga aggtgagcac
     1921 gagctggatg aagaagagcg cggtgaaggg aattgcgata taccagaaga ctttttcaaa
     1981 catgagcatg ccgctccacc actcccccac tcacaccgcc tcctttgcgc cgacagggtt
     2041 tcgcatgtgt cgtcagctgc ggattcgcag gctgaacttg accacgacct cttcgtcggg
     2101 tttggcgggg acgtcgaagc ggagcgtgaa ggcgtcggtc tgttcgtacg gatgggtcga
     2161 tgcgatcact tcccagtcgc cccagacggg ctcgttgaag gagacgacga cagactcatc
     2221 ctttttgcgg tttctgacga taagctccca cgacgtctcg tagaggttgt cggccagctt
     2281 cttgtagcgc gtctgacggc gttccgcgac cacgtcgaag gcctcgccca ccttcagacg
     2341 gacctcctcg ttgcgcggcg tgtggtcgat gcggtcttcg ccgatgaact gaaggcttcc
     2401 tttggagtct ttcttgtaca tcctgatgat ccccttggga aggggcatgc cgaggttgtt
     2461 ctcctcggaa tttttgaagg agatgacgac gtcgaccttc tgcttcagca gcccccgctg
     2521 gagcgaggag gtgaagtagc gccgctcgcc gcgggtgatg tactccttgc cgattttcgt
     2581 gccgaacgcc tccagcaggc tgatctgctt ggtctggttg ttccctatcg tcgtctttcg
     2641 cgccagattg tagatgtggt actcgaaaaa agcctcttct tcgaaggcag gcgcggcttc
     2701 gggtaccccc ttccccatcg ccatccgcat catgggcgga ggaagaacgt cggcgaccgt
     2761 ctgcacttcg ccggcgatca gcttgagcgt cgcgtcccgg tacgccgcgc cgcttctgtt
     2821 gttcagcgtg acccagccgg tgaggtcggc cgctgtatcg gcgtcgtcga ggacgaagat
     2881 gtagtccgcc ttccacgtca tcccgctcgt caggtaggag acctcgacgc tgtgcttctc
     2941 cttcgccgag ttggcgtaga gccacgccaa agtggggcgc gcatagaggt tgtcagggat
     3001 ggagggaaca atcttcagtc cggggtagtt caggtagatc tggccgccga tgtcgaagac
     3061 gagtccgtta ttcgtgctca gaagcgtgcc ctccacgatc tccttcttcc cctggtagtc
     3121 gttccacacc tcgatcttca ccgtgcgccc gacgaacttc tccagcagct tctccgggct
     3181 gatgaggtcg tactcgtagt tctgctccag aatcgccaga gaaggcgggt tgttgagcga
     3241 cttcacatgc accgtctccg gccggatgga agaagccacg tccatgaagc ggagctcccc
     3301 ctgcccctct ttgagcagga tgctcctgac gtccttcacc agagcaagat ccgtgttgta
     3361 caccgtgacc tcgacccccg tctgatctgc ttccgtgctc gagaccacct ccgcccccag
     3421 tgcaggcaga gcggagagaa agacgaacag aaaaaggaac gtgcacagta tctgaatgct
     3481 tttcatttca cagacctcct tttttagttt gccgcaattt ttaggaacac acgctctgtt
     3541 catcgctctc cggctgcgtc ccgcccgcgg attccgcgag ggcgtcgagc agaaggagca
     3601 ggcgcatcag gtagagcagc acgccgccgt tcgcggcgag gatgacgccg atgagcaggc
     3661 cggggatgat cccgggcgcg agcatcgacg agccgaacat gaccgagagc cccccgaggg
     3721 cgatctgcgt cgagtacagc ctgaagag
//
LOCUS       DAOSWW010000150         3746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17234_length_3746_cov_3.489840, whole genome shotgun sequence.
ACCESSION   DAOSWW010000150 DAOSWW010000000
VERSION     DAOSWW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17234_length_3746_cov_3.489840"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PK442_04250"
     CDS             complement(<1..938)
                     /locus_tag="PK442_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO86890.1"
                     /translation="MTRRFGSPWFLVFIFFGVVIWRLTSLQLFPDPRVENQSRRQYWI
                     HVPVSTNRGFIYDRNGNALAVSVPSFSFFLDPAFWNPSDAVHLKGILPDSLVDTLSKP
                     LSGRFLWLARKVDIETAEKIRSLKIKGIFEIEEKKRLYPSGSLLSHVLGFCDIDDKGL
                     SGIELMWDKALFSPPGLRVLAKDSSGRTLDISRNDHDHTPTEGGNVSLTIDSRIQFIV
                     ERRLEEGIREHSARWGSIICMDPQTGAILSMASWPTFNPNVRTDLSKTQNLLNNSIGR
                     TYEPGSTFKPVVLGISMERGSVHPNESFHCPYRIKIA"
     gene            complement(970..1389)
                     /locus_tag="PK442_04255"
     CDS             complement(970..1389)
                     /locus_tag="PK442_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86891.1"
                     /translation="MRFREDIKKETVSDSFHKISPYIIVFTLCAFGLFMGLGVLRLYS
                     FRLECKLNSVNRQIESYKANRIVLKHELSSLVSPARIYGFSKQEMGMTFASNVRILRV
                     EEHLLAEASFESSPVVASKQENEGWFFFFLEGAMAGE"
     gene            complement(1397..2299)
                     /gene="rsmH"
                     /locus_tag="PK442_04260"
     CDS             complement(1397..2299)
                     /gene="rsmH"
                     /locus_tag="PK442_04260"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOO86892.1"
                     /translation="MEHVPVLLKEILAFLQEHPSLRSVLDCTLGLGGYSEAILESFES
                     TEVYAVDQDSHALSFAKERLLPFGARFHPEKGNFASLPPAVTCNAPFDAMLFDLGVSN
                     LQLVDEDRGFSFQSDGPLDMRMDREFRYSAADLVNSYSEREIADIFWKYGEERFSRQL
                     ARGIVRYREESGKIERTSTLVDIVRKTLPAPVQRKMGGHPARRIFQALRIAVNDEISI
                     LETGLHNIPDVAAPGALLVVVSYHSLEDRIVKNLFKKWREEGKGSIVTKRPLVPSDEE
                     IEANHKSRSAKMRVFSFKPKRMEV"
     gene            complement(2299..2724)
                     /gene="mraZ"
                     /locus_tag="PK442_04265"
     CDS             complement(2299..2724)
                     /gene="mraZ"
                     /locus_tag="PK442_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOO86893.1"
                     /translation="MLVGTYEHKIDSKARIVLPSKFREELGESVFATIGIDKCVSLYS
                     PEEWKKVMERLQELSFSKSKSRGLLRLMLSSAHELPVDGAGRILLPPVLRDYAEFIQE
                     VVFVGVSDHVEMWDKDKWNDYRKQVLDDLPEIAEGVEGF"
     gene            complement(2868..3200)
                     /gene="rnpB"
                     /locus_tag="PK442_04270"
     ncRNA           complement(2868..3200)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK442_04270"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3324..>3746
                     /locus_tag="PK442_04275"
     CDS             3324..>3746
                     /locus_tag="PK442_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOO86894.1"
                     /translation="MIEKTSYDSPEYDVFKSNVKKLTGLDLNSYKNQIHRRVHMLMQR
                     WSVSSYDIFLKTIKENDQKLREFLDYLTINVSEFFRNPNKWTELQDVVIPRLIQSRGK
                     KQLKLWSAGSATGEEPYSLAILSMEAKLAPQTPVLASDI"
BASE COUNT         1022 a          903 c          868 g          953 t
ORIGIN      
        1 gcaattttga tcctataggg gcagtggaat gattcattcg gatgaacgga gcctctctcc
       61 atgcttattc caagaacaac gggcttaaac gtcgagccgg gttcatacgt acgcccgata
      121 gaattgttta ataaattctg cgtcttcgat agatccgttc gcacatttgg gttaaacgtc
      181 ggccaactcg ccatcgaaag aatggcgccc gtttgaggat ccatacagat aatgctgccc
      241 caccgagcgc tatgttcgcg aataccctcc tcaagacgcc gctccacgat aaactggatt
      301 ctggagtcta ttgtgaggga gacattccca ccttccgtag gtgtgtggtc atggtcattt
      361 ctgctgatat caagagttcg tcccgacgaa tccttggcaa ggacacgaag acccggggga
      421 gaaaacaacg ctttatccca catgagttca atacctgaaa gccctttgtc gtcgatatcg
      481 caaaatccga ggacatgtga gagaagagag ccgctcggat agagtctttt tttctcttct
      541 atctcgaata tacctttaat tttgagcgag cgaattttct cagccgtttc aatatcgact
      601 tttctcgcta accataaaaa acggccagaa agaggtttcg agagcgtatc gactaaacta
      661 tcggggagta tccctttgag gtgtactgca tctgaaggat tccaaaaagc gggatcaaga
      721 aaaaaactga atgaaggaac ggaaaccgca agggcgttcc cgttcctgtc ataaataaag
      781 cctctattgg tactgacagg aacatgaatc cagtactgtc ggcgcgactg attctcaacc
      841 ctcgggtcag ggaagagctg aagagatgtc agcctccaga taacgacgcc aaaaaagata
      901 aaaactagaa accaagggga tccgaaacgt cgcgtcacaa aaacaccctc cgttcaggat
      961 acacgggaat tactctccgg ccattgcgcc ttcaagaaag aaaaagaacc agccttcatt
     1021 ctcctgtttc gaagcgacaa caggagaact ctcgaaagaa gcctcagcta aaagatgctc
     1081 ttctacgcgg aggatgcgga cattagatgc gaatgtcata cccatctctt gctttgaaaa
     1141 accgtagatg cgagcagggg aaacaagtga tgagagttca tgtttaagta caatacgatt
     1201 tgctttgtat gattctattt gcctgttgac agaattcagt ttgcattcca gacggaaaga
     1261 ataaagacga aggactccga gtcccataaa gaggccaaac gcacagagcg taaaaacaat
     1321 aatatatgga gagattttgt gaaaggaatc agaaacggtt tcttttttta tatcttcgcg
     1381 aaatcgcatg tcctttttac acctccattc ttttcggttt gaatgaaaaa accctcattt
     1441 tggcgcttcg cgacttatga ttcgcttcaa tctcttcatc tgaaggaacg agaggccgct
     1501 ttgtcacaat acttcctttc ccctcttcac gccatttttt aaacagattc tttacaatcc
     1561 tgtcttcaag agaatggtag ctcaccacga caagaagtgc gcccggcgct gcgacatctg
     1621 gaatattgtg taaaccggtt tcgaggattg agatttcgtc gtttactgct atacgtaggg
     1681 cttggaaaat tcttcttgcc ggatgccccc ccatttttcg ttggaccggg gctggcaatg
     1741 tttttcgaac gatatctaca agtgtggacg tgcgttcgat tttgcctgat tcttctctgt
     1801 aacggacgat tcctcttgcg agctgtcttg aaaagcgctc ctccccgtac ttccaaaaga
     1861 tatctgcaat ctctctttct gaataagagt tcacaaggtc ggcggcagaa taacgaaatt
     1921 ctcggtccat ccgcatatcc aaggggccgt ctgactgaaa cgagaaccct ctgtcttcat
     1981 cgacaagctg gaggttcgat actccaagat cgaacaacat cgcatcaaat ggtgcgttgc
     2041 aagtaactgc aggaggaagg gaggcaaaat tccctttttc agggtgaaaa cgggcgccga
     2101 aaggaagaag ccgttctttt gcaaacgaaa gggcatggct gtcctgatct acggcatata
     2161 cctctgttga ttcgaaagac tcgagtatcg cttccgagta gcctccgaga ccaagagtac
     2221 agtcaagaac agagcgaagg gaagggtgtt cctgaagaaa agcgagaatt tcttttaaga
     2281 gaacgggaac atgctccatc agaacccctc cactccttcg gcaatctcag ggagatcgtc
     2341 aaggacctgt tttctatagt cgttccattt gtctttgtcc cacatctcta catgatcact
     2401 gacgcctacg aacactacct cctggatgaa ctcggcataa tcgcgtagta ccggagggag
     2461 cagtattctg ccggcgccgt cgacgggaag ctcatgagca ctcgaaagca taagacggag
     2521 caatcctctg ctcttgcttt tcgagaaaga gagttcctga agacgttcca tcactttttt
     2581 ccactcttcg ggagagtaaa gggagacaca tttgtcaatg ccgatggttg cgaagacgct
     2641 ttccccgagc tcttctctga acttcgacgg aagtacaatt cttgccttgc tgtcgatttt
     2701 atgttcatat gttcccacca acatgccagt gcctcccact ttgttacact ctctcccact
     2761 agaacccaca gtatcattga ttgcaaaaga tacgtaggcc tcaggaccct tgaataatac
     2821 aattaatatg ggtcctgagg cctatttgtt tggtgagaaa aattgtaaag agggagcatc
     2881 ctgtaagccg ggttctgtcg tgggtgacca tttatctgaa gaacaccttg cggcgttctt
     2941 ttcgcgaccg tacccgaggg tcggcgggca gcctcgtccc ctcctattcg gtcttgctcc
     3001 ggacggggtt ttcctggcat ggaaatcact ttccacccgg tgggctctta ccccaccttt
     3061 tcacccttac cctgaaaagg gcggtatatt tctgtggcac tttcctttgg tttgcaccaa
     3121 ctgggtgtta cccagcgtcc tgccctgtgg agcccggact ttcctctctt caaaaaagag
     3181 cggccaccaa ggatactccc ggaggcacat tatatcatct tttccgagag agaacgaaat
     3241 ggtttttcga gcacaaaatg cgatacaata gagaacgaag cgcgaaacgg gtgtgcatta
     3301 tcgagcgaag gagccagagt gtaatgatag agaaaacgag ttacgattcc ccagaatacg
     3361 atgtattcaa gagtaatgta aaaaaattga cagggcttga ccttaattcc tacaaaaatc
     3421 agattcatcg ccgcgtacac atgctgatgc aacggtggag tgtgtcttcc tacgacattt
     3481 tcctcaaaac tatcaaagaa aatgatcaga aactgcgcga gttcctcgat tatcttacaa
     3541 taaatgtttc ggagtttttt cggaatccga acaagtggac ggaattgcag gatgtagtaa
     3601 tcccaaggct gatccaatca agagggaaga aacaactgaa gctttggagc gcggggtcgg
     3661 caacaggcga agagccgtat tcgcttgcga ttctgtcaat ggaggcaaaa cttgcacccc
     3721 agactcccgt tctcgcaagc gatatc
//
LOCUS       DAOSWW010000151         3743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17258_length_3743_cov_3.074024, whole genome shotgun sequence.
ACCESSION   DAOSWW010000151 DAOSWW010000000
VERSION     DAOSWW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17258_length_3743_cov_3.074024"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PK442_04280"
     CDS             <1..693
                     /locus_tag="PK442_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOO86895.1"
                     /translation="VSEAGVSPLEGARQLVVVVAEDWSSVPAVLQRYERDGDTAPWRL
                     VGKRIQVSLGRSGQGWGIGLHPEEARTPGEPEKREGDGRAPTGVFELVSCFAYAPEEL
                     AGTAMPVLKADSELVCVDDSSSRYYNEVFPGNVPDKDWNSAEDMLRKDGLYRYGVVVA
                     HNQNPPLPGRGSCIFLHVERSPGAPTAGCTAMKQSAVKELILWLDPAAKPMLAQFPKD
                     VYERLKEEWAMP"
     gene            918..1475
                     /locus_tag="PK442_04285"
     CDS             918..1475
                     /locus_tag="PK442_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86896.1"
                     /translation="MLITSVLFRRKLVPLFALFVLLFGVFPANAELNRITEAEILDAQ
                     KGWAEGVVSIGRAYVDKKDYKAAAAEFVDAFYGYDQGPVLFKPTKASAVKFRLTRGEA
                     LSYFVKGSVPEDHGFVLQPWSNVRFENAGIVIDGDSALAMGVYFFTDANTSEEVEVEY
                     TFGYFKDESGKIRIRLHHSSVPYSE"
     gene            complement(1596..2387)
                     /locus_tag="PK442_04290"
     CDS             complement(1596..2387)
                     /locus_tag="PK442_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOO86897.1"
                     /translation="MKTTEAYPSHLLIGQFSKLSGLSCRMLRFYAEQNILVPAYTDCS
                     TGYRHYLASQLTEAKLLKQLRQADFSLGDIAEILNGLKTTQWEMCVAAQRESIARKIE
                     SMEMALEELEQLEKYQGPDECILAHVAPMRVRSAPLAGERLRLREIARSEAKHIFGED
                     GGSSPLLCVYDLQTGPASATICIPAEEPDGEVMPGCVLAVSLNRGAYETIPATLEQLL
                     AWVERNQYMTNGKVVERYFPCGEEDHPHGRVEIAVALDDDSHTSQ"
     gene            complement(2384..2653)
                     /locus_tag="PK442_04295"
     CDS             complement(2384..2653)
                     /locus_tag="PK442_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86898.1"
                     /translation="MDEFGRSLRSFGLNPDKVRDALRCGDIREVLDSLKKEEETLLLR
                     RVEIADLRERLLAMAGEERLRIHDEISDDDLELMSAAGQEPRNIP"
     gene            2939..3217
                     /locus_tag="PK442_04300"
     CDS             2939..3217
                     /locus_tag="PK442_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HOO86899.1"
                     /translation="MSVEAAAAFYDRLENDLALMGKLKELGDKERLVLYVKDELGYDF
                     SREEMRKVLFERNPELSEEELQSVVGGAGGNGGSDGLSVANVIVTAGL"
     gene            3299..>3743
                     /locus_tag="PK442_04305"
     CDS             3299..>3743
                     /locus_tag="PK442_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86900.1"
                     /translation="MRKYRGMPMVLVVLLMTAVMFGGGCGGGSDRMPAHPGEIQPGAE
                     YEGVLSVQDGDEDRVLMRTRYEAHNIETGDSMEPVVALFVKEEEHLDEATGTMRVLSA
                     SEISRPTPEQVKAFLKDFWDAEHGGPADEGVNAREFLDFLAAHNMS"
BASE COUNT          777 a          950 c         1178 g          838 t
ORIGIN      
        1 gtttcggagg ccggtgtctc gccgcttgaa ggagcccggc agcttgtggt tgtggttgct
       61 gaggactgga gcagtgtgcc cgccgtgctg cagcgctacg agagggacgg ggatacggct
      121 ccgtggcggc tggtcgggaa gagaattcag gtgagtctgg gccgcagcgg tcaagggtgg
      181 ggaataggac tgcatccgga ggaggcgaga acgcccggcg aaccggagaa gcgcgagggc
      241 gacggccggg ccccgactgg ggtgttcgag ctcgtttcct gtttcgccta cgctccggag
      301 gaactcgccg gaacggcgat gcctgtcctg aaggcggatt cggagctggt gtgcgttgac
      361 gattcctcat cccgctatta caacgaggtg tttcccggga atgtcccgga caaggactgg
      421 aacagcgcgg aagacatgct ccgcaaggac gggctgtacc gctacggcgt cgttgtggcg
      481 cacaaccaga atcccccgct tccgggcaga ggatcgtgca tcttcctcca tgtggagcgc
      541 tctcccggcg cgccgacggc ggggtgcacg gcgatgaagc agagtgcggt gaaggagctg
      601 atcctgtggc tcgaccccgc ggcgaagccg atgctggcgc agttcccgaa ggatgtgtac
      661 gagcgcctga aggaagagtg ggcgatgccg tagggcgggg tttttctctg taggagcctt
      721 gggtgaagag cggaaaaaga ataagaaaat ttactgaaat aaaaaattcc gaaaagtgcg
      781 cttgacagtc atactccagt atggtataaa aattatacca taagagcttg cggctcttct
      841 tacatcgtta catccagaaa cgggtccggc gaatgcgacc ggacgatgaa gaggtggagt
      901 acgaggaggt gttccgtatg ttgataacga gcgtgctttt ccggagaaaa ctggttcccc
      961 tgtttgcgct gtttgttctt ttattcggcg tgtttcctgc aaatgctgaa ctgaatcgaa
     1021 tcaccgaggc ggagatcctt gatgcgcaaa aaggatgggc ggaaggcgtc gtctccatag
     1081 gcagagccta cgtcgacaag aaagactaca aagccgcggc ggcggaattc gtcgatgctt
     1141 tttacgggta cgaccaaggc cctgtactgt tcaagccgac gaaggcttcg gcggtgaagt
     1201 tccgtttgac gagaggagag gcgctctcct atttcgtgaa gggaagcgtc cccgaggatc
     1261 acggattcgt tcttcagccg tggagcaacg tgcgctttga gaacgccgga atcgtgatcg
     1321 acggcgactc cgccctggcg atgggcgtct atttttttac cgacgcgaac acatccgaag
     1381 aggtggaagt cgaatatact ttcgggtatt tcaaggatga gagcggaaag attcgtatca
     1441 gactccatca ttcctctgtt ccgtattcgg aatagagcag gaaaaacgat tcgggagata
     1501 gcataacgaa tggccggaga ggatgcgagt ccgctccggc cattcgtttc gtcttcaggt
     1561 gagaatcacc cgtttggttg atgtatttgt accgtctact gcgaggtatg agagtcgtcg
     1621 tccagcgcga cggcgatctc gaccctgccg tgggggtgat cttcttctcc gcaggggaag
     1681 taccgctcga caactttccc gttcgtcatg tattggttcc gttccaccca cgcaagaagc
     1741 tgctccaggg tggccggaat cgtctcgtag gcgcctctgt tgagcgagac ggccagcacg
     1801 catcccggca tgacctctcc gtcgggttct tccgccggga tgcagatcgt cgcactcgcc
     1861 ggacctgttt gcaggtcgta gacgcacaaa agaggcgagc tgccgccgtc ctctccgaag
     1921 atgtgctttg cctcggagcg tgcgatctcg cgaaggcgca accgttctcc ggccaacggg
     1981 gcggaacgta cgcgcatcgg ggcgacatgc gccagtatgc actcatccgg cccctggtat
     2041 ttttcgagct gttccagttc ctccagtgcc atctccatcg actcgatctt ccgggcgatt
     2101 gactcgcgtt gtgctgcgac gcacatttcc cactgtgttg tcttcaatcc attcagaatc
     2161 tccgcgatgt cgccgagtga gaaatctgct tggcgaagct gcttcaggag cttcgcttcg
     2221 gtgagctgag aagcgagata gtgtcggtag cccgtcgagc agtcggtata tgccggaaca
     2281 aggatgttct gctctgcgta gaagcggagc atacgacagg aaagcccgct taactttgag
     2341 aactgcccga tgagcagatg agacgggtaa gcttccgtcg tcttcatggg atgtttctcg
     2401 gctcctggcc cgccgcgctc atcagttcaa gatcgtcgtc ggaaatctcg tcgtgtattc
     2461 tgagtctctc ttctccggcc atggcgagca gccgctcgcg gaggtcggca atctcgacgc
     2521 gtctcagcag gagcgtctct tcctcttttt tcaaggaatc cagaacttcg cggatatcgc
     2581 cgcaacgcag ggcgtcgcga actttatccg gattcaatcc gaacgaacgc agcgaccgac
     2641 cgaactcatc catttttttc ccccctttgc ggtacccgat tttcggcgtt atcgtacacc
     2701 ttcacatagt gtgagagtca aggctccgaa tattcttcgg agagccgccg ttgatacagg
     2761 gcgccgtcaa agtacacaaa gtatggcacg aaggcgcgcc tccttcaagt aggggcggca
     2821 ctatttttta ttttcgcact tgacattgac acagtgtcaa tgtttatact gtcggcagta
     2881 agacggggac tcctgttttt cctgcacgct attctacgta cgatgggggg tggtctgtat
     2941 gtcggttgaa gctgcggcgg cgttttacga ccgtctcgaa aacgacttgg cgcttatggg
     3001 aaaattgaag gagttaggcg acaaggagcg attggtgctc tacgtgaagg acgaactcgg
     3061 gtacgacttc tcacgggaag agatgcggaa ggtgctcttc gagcggaatc cggaactgag
     3121 cgaagaggaa ctgcaatcag tcgttggagg cgcggggggc aacgggggat ctgacgggct
     3181 ttctgttgca aatgtgatcg ttacggcagg tctgtaactc ggattcctct tcgttttccc
     3241 gaagaagaga gcatgtgtgt gaatcgctgg ttattttgat tgaaaggagt ggttgctcat
     3301 gaggaaatac cgcggaatgc ccatggtgtt ggtagtgctc ttgatgacgg cggtgatgtt
     3361 cggaggggga tgcggcggcg ggtcggacag aatgccggcg catcctgggg agattcagcc
     3421 gggagcggaa tacgagggtg tgctttcggt tcaggacggc gacgaggacc gggtgctcat
     3481 gcgcacccga tatgaagcgc ataatatcga gaccggcgat tcgatggagc cggtggtcgc
     3541 gctctttgta aaagaggaag agcaccttga cgaggcgacg ggtacaatgc gcgttctgtc
     3601 cgcgtccgaa atctcaaggc cgactcccga acaagtgaag gcgtttctga aagacttctg
     3661 ggacgcggag cacggcggcc ctgcggacga aggcgtcaac gcgcgtgaat ttctggattt
     3721 tcttgccgcg cataacatgt ccc
//
LOCUS       DAOSWW010000152         3704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17540_length_3704_cov_3.846259, whole genome shotgun sequence.
ACCESSION   DAOSWW010000152 DAOSWW010000000
VERSION     DAOSWW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17540_length_3704_cov_3.846259"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK442_04310"
     CDS             <1..203
                     /locus_tag="PK442_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOO86901.1"
                     /translation="VNKPSTPPEPRRYRQGGYIRRLPVDPWGSQYVYRMPGDHAEFDL
                     FSKGPDGEEGGEGAGKDISNWE"
     gene            458..829
                     /locus_tag="PK442_04315"
     CDS             458..829
                     /locus_tag="PK442_04315"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HOO86902.1"
                     /translation="MKKNFAVLLTVALVCVLSLYAFAGAAEQPLSVTGGEPVVYDCNN
                     GDEVVARYYMLSDESLRFVKLLLPDGTEYTLPQVLSGSGARYTDDIELLWWIKGDDAY
                     VEKRDETGEWRPLYSECRARP"
     gene            complement(996..1238)
                     /locus_tag="PK442_04320"
     CDS             complement(996..1238)
                     /locus_tag="PK442_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86903.1"
                     /translation="MASFPQATARVAVGDLIGKIRAHYGKSVETNIYDPRCLFWFFDL
                     VRFNIRAEPTWVFDGKLLFRGIPNWEELREKMDATL"
     gene            complement(1332..2111)
                     /locus_tag="PK442_04325"
     CDS             complement(1332..2111)
                     /locus_tag="PK442_04325"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOO86904.1"
                     /translation="MKESSVDLYFFSGTGNTLLAARAVAEGLRAGGKSVRLLRLEKGI
                     SAPAGDTALGVAVTTACFSTYPFVWEALEQLPSGEGRSAFAISTMGGYSGGLRSPLRK
                     LLHKKGYRTLGYAEFVMPSNYANKSIPQEANRVKIQKCEAKAREFALALLEDRATWRS
                     GGPFSPFFNWMARKKAPWRMMKRSFPLEIDKSRCIQCGKCARLCPAKNIVMEEYPRFL
                     DHCVVCQRCIAFCPPAAIGVHGKKYEQYRAVEYDTLVSDKL"
     gene            2257..2388
                     /locus_tag="PK442_04330"
     CDS             2257..2388
                     /locus_tag="PK442_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86905.1"
                     /translation="MRILESLLLFALFFSAEKAYAAFRPPLDESAPEVFQTASFALG"
     gene            2404..2961
                     /gene="msrA"
                     /locus_tag="PK442_04335"
                     /pseudo
     CDS             2404..2961
                     /gene="msrA"
                     /locus_tag="PK442_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
     gene            complement(2972..3400)
                     /locus_tag="PK442_04340"
     CDS             complement(2972..3400)
                     /locus_tag="PK442_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOO86906.1"
                     /translation="MEPAALLRAREIRPTPLRKEALALLFAAGVPLSLKTLSERFQGK
                     PPDRVSLYRTLTLLELKGIVHKVLGADGAWRYCAHPPEESGCPGNHAHFLCLECGSMF
                     CLQDQPIPVLTLPDGCVVEGKQLLAYGLCKECAELRTKGK"
     gene            complement(3400..>3704)
                     /locus_tag="PK442_04345"
     CDS             complement(3400..>3704)
                     /locus_tag="PK442_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOO86907.1"
                     /translation="SVDADDRTGVAIAQVSDSVVFNGVPALPGARLMLALQDKTKIIG
                     APACVVHDEWTSMDILLNRLFAGLIPTLSEVRRWGAGGLCRKCRTCNYPVCAFAAR"
BASE COUNT          797 a         1008 c         1065 g          834 t
ORIGIN      
        1 ttgtgaataa gccttccacc cccccggaac cgcgtcgcta ccgccagggc gggtatatca
       61 gacgcctccc tgtcgatccc tggggaagcc agtatgtgta ccgcatgccc ggggatcatg
      121 ccgagtttga tctcttctcg aagggaccag acggagaaga gggcggcgaa ggagcaggaa
      181 aggatatttc gaactgggaa tagacaacta tgtgtatccg ggtcgcctca cgaaacagag
      241 gcggcctttt ttatgcgcac tcgtccataa acgggccatt gagggaaaaa ccgggaggat
      301 gtgtcgtttc acggggagat agccgcaggt tcactctcca ggaacccgaa tagggctgcg
      361 agttttgctt cttgcaacgc acccgggaag tgcgtattat tgaataacgc ttgagacggc
      421 gattcaaatc cctttcgggg gagaaggagg gggaagcatg aaaaagaact tcgcagttct
      481 gcttacggta gcgctggtct gcgtcctttc gctgtatgct tttgcgggcg ccgcggagca
      541 accgctgtcc gtcacggggg gcgaacctgt ggtgtacgat tgcaataacg gggatgaggt
      601 tgtcgctcgg tattacatgc tctctgacga aagtctccgt ttcgtcaagc tgctcttacc
      661 ggacggaacc gagtatacgc tgccccaggt gctttccggc tcgggagccc ggtacactga
      721 cgatatcgaa ctgctctggt ggataaaagg agacgacgca tacgtcgaaa agcgggacga
      781 gacgggcgaa tggcgtcccc tgtacagcga gtgccgcgca cggccgtaac ggttttgaac
      841 tgacggaaga agctccctgc acggtttgcc ctggatcaat gcgccgagtt ccgtgcacgg
      901 gagcttcctt tctggagacg aagcgtatga acctgatgtt gtcatgaccg cgttcggtag
      961 tgtggagtac ccaacttgct gcttcgcagc gcggttcaaa gcgtcgcatc catcttttcc
     1021 cgcagttctt cccagtttgg aatccccctg aagagcagtt tcccgtcgaa aacccacgtc
     1081 ggttctgctc ggatattaaa ccgcaccagg tcgaaaaacc agaaaaggca tcgggggtcg
     1141 tagatatttg tctcgacgct cttgccgtaa tgcgctcgaa tcttgccgat cagatctccc
     1201 accgccacac gggcggttgc ctgcgggaag gaggccatct cctcgcttcc gtacagaaat
     1261 ccgcttcagc agacggagga aatcggtttt tccatgtaga ttttgagacg atgccgcata
     1321 ggcttcactc cctatagttt gtcggatacg agcgtatcgt attccaccgc cctgtactgt
     1381 tcgtacttct tcccatgaac gccgatggcg gcaggagggc agaaagcgat gcatcgctgg
     1441 cagacgacac agtgatcaag gaaacgcggg tactcctcca tgacgatgtt tttcgcaggg
     1501 caaagacggg cgcactttcc gcactgaata cacctgctct tgtcgatttc gagcgggaac
     1561 gagcgtttca tcattctcca tggcgctttc tttcgcgcca tccagttgaa aaacggcgaa
     1621 aacggtcccc cgctgcgcca tgtggcgcga tcttcgagaa gcgcgagcgc aaattcgcga
     1681 gccttcgcct cgcatttctg gatttttacc ctgttcgctt cctgcgggat actcttattc
     1741 gcgtagtttg acggcataac gaattccgca tacccgagcg tcctgtaccc ctttttgtgg
     1801 agcagtttac gaagaggcga tcgcagcccc cctgaatacc ctcccattgt ggagatcgcg
     1861 aatgcgctcc gtccttctcc tgaaggaagc tgctccagag cctcccagac gaacggatag
     1921 gtcgagaaac atgcggtcgt tacagcgacg ccgagcgccg tatcccccgc cggcgccgat
     1981 atcccctttt cgaggcggag caagcggacc gattttcccc ctgcgcgaag cccttcggct
     2041 accgctcgtg cagcgagaag tgtgtttcct gtaccagaga aaaaatacag atccacggat
     2101 gattctttca ttgtgtctcc tttcttcttt ccttacggag agtgatgatg aagtaattct
     2161 atcacttccg ccataatgct gatcttttac agggtacaat ctgagctaaa gtagtgtatc
     2221 atagtggatg aaacaaagaa tttggaggtg agagagatga gaatattgga gtcgttgctt
     2281 ctttttgcgc tgttcttctc cgcggagaag gcgtacgctg cttttcgtcc tcctctggat
     2341 gaaagcgctc cggaggtttt tcagacagcg tcctttgctc ttggctgatt ttggggaccg
     2401 gatgcccggt tcgggcttct tgaaggcgtg atccgtaccc gcgtcggcta tgcgggagga
     2461 acgacgccgg accctaccta cgaagagata ggagatcatt cggagacaat tcagatcgat
     2521 tacgatcccg aagcgctctc gtacgaagag ttgctgagtg tcttctggag cagtcataac
     2581 ccgtcggctc cctcctggtc gacgcagtac cgttccgcca tctttgtagc aaacgaagag
     2641 cagcgtcgtg cagcggagga aagccgtgaa aaggtgaaaa cgtctcgcgg aagtggagtg
     2701 cagacttcca tagagccgct gaagcgcttc tatccggcgg aagactatca ccagaagtac
     2761 tacctgcggc aggagcacgt gttgatgacg gatttcatga ataactacgc cacttcggag
     2821 gattttatga actctcccgc ggcggcgaag gtgaacggct tcctttccgg gactggttca
     2881 gaggagtatt taaaaaaaat actccccggt cttggtcttt cggaagaagg ggagaagaga
     2941 ctcaggcttc gcgtccgttg aacaggagcg tttatttccc ctttgtgcga agttctgcgc
     3001 actccttgca gagaccgtag gcgagcagct gtttgccctc taccacgcag ccgtccggta
     3061 gcgtaagcac aggaatgggc tggtcttgaa ggcagaacat ggagccgcat tccaggcaga
     3121 gaaagtgcgc gtgatttccg gggcagccgc tctcttccgg agggtgcgca cagtatcgcc
     3181 aagccccgtc cgctccaagg actttgtgga cgattccttt cagctcaagg agcgtcaatg
     3241 tcctgtacaa cgaaaccctg tcgggcggct tcccctggaa gcgctcggac agggttttga
     3301 gagaaagagg gactcccgct gcgaaaagaa gcgcgagcgc ttcttttcgc agcggcgttg
     3361 gacgaatttc tcgtgccctc aggagcgcgg cgggttccat tacctcgcgg caaaggcgca
     3421 cacgggatag ttgcatgtac ggcactttct gcaaagaccg cctgcgcccc atcggcggac
     3481 ttcggagagc gtggggatga gccccgcgaa gagacggttg agaaggatat ccatgctggt
     3541 ccattcgtca tgcacaacgc atgccggagc tccgataatc ttcgtcttgt cctgaagcgc
     3601 aagcatcagg cgggcgcccg gaagtgccgg aacgccgttg aacaccacgc tgtcggagac
     3661 ctgggcgatt gccaccccgg tccggtcgtc cgcgtcgacg ctca
//
LOCUS       DAOSWW010000153         3665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17813_length_3665_cov_4.217729, whole genome shotgun sequence.
ACCESSION   DAOSWW010000153 DAOSWW010000000
VERSION     DAOSWW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17813_length_3665_cov_4.217729"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..2455)
                     /locus_tag="PK442_04350"
     CDS             complement(209..2455)
                     /locus_tag="PK442_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HOO86908.1"
                     /translation="MKTPAVAPHCRLQTVPGEGALALSERGAKAFYGAAYELVVPLID
                     GCRSADDIADALAGSVDAATVYYVLNILEQRGCVAEATPEIPPALSAFWHEAGVEPRA
                     AMEALRRSSVAVFVAGNLDSSPLETALKEAGVRTGPKESADLWVVVADDYQRDELREI
                     NGEALAARRPWLLLRPTGPEAWIGPLFLPGETACFRCLLSRLERNRSAHALAKRLGGG
                     KAVPSTSVAALLSTLASAYGMAATEAARFLAGASRTLAGKLLSLDWTDWSARTHALVR
                     HPYCPACGTEAPREAKPLPLEPGRATFNKDGGRRAVSPERTLKRYEHLISPITGVVKE
                     LRPVRSDDDVAVVYAAGHNTALKMDRLDSLKRSLRSSSSGKGASEAQARASALCEAVE
                     RYSGEWTGGELRIPRALNDWSEGEAIHPNDVMRFSERQYAEREVWNSRGSRFNRVPEP
                     FDPERIVDWTPVWSLTEERHKYLPTQLLYYRAPARDGDETVYSIGCSNGAASGNTLAE
                     ALLQGFFELVERDAVAVWWYNMLSRPGVDAESFGDPWIVKLAAYYRERWGRETWALDI
                     TSDLGIPAFVAVSRRIGEREERLFFGLGCHLDARIALQRAFAEMNQMLSVGSGGGEDD
                     LAGLQDKETLSWMRTATLANKPYMAPDPSKPLRTFSDYPVRHTDDFLEDIAYCRRLIE
                     AKGMEMLALDQTRPDTGMPVIKVVVPGLRHFWARFAPGRLYDVPAATGLLKKTLKEEE
                     LNPVPFFF"
     gene            complement(2526..2810)
                     /locus_tag="PK442_04355"
     CDS             complement(2526..2810)
                     /locus_tag="PK442_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HOO86909.1"
                     /translation="MDRKEMRKVLAKLIAKAWADARFKAKLMTDPASVLRAEGVAIPE
                     GVKIDVFENTDERIHLVIPKKPDELSDEALDIAAAGGQYSWDDNNCFITW"
     gene            complement(2890..3168)
                     /locus_tag="PK442_04360"
     CDS             complement(2890..3168)
                     /locus_tag="PK442_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HOO86910.1"
                     /translation="MDRKEMYKATGKLIAKAWVDAQFKAKLMADPASVLRAEGFSIPE
                     GMRIDVFENTDERIYLVIPKKPDQLSDEELDIAAAEGQNDVFCSCDFY"
     gene            3390..>3665
                     /locus_tag="PK442_04365"
     CDS             3390..>3665
                     /locus_tag="PK442_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86911.1"
                     /translation="MKMHEEARRAGTEKNAPAGEGEISAPLFDPESLAVSLKRGVRLH
                     ETDGRAVLVDADGVALALPLSPETRRIFLSALDEGVCTADELGDAALH"
BASE COUNT          652 a         1170 c         1132 g          711 t
ORIGIN      
        1 ttgctgcgcg tgcggcaagc gtggatattg aagcggcgcg gaacgacgtg cgtccttttc
       61 tccgtgatcc ccgggcgctc tccttctgga gcaacgagct tttcgcgtcg cttgttgaac
      121 gatttcgctt ctcgaagcac ccctctgcga aagactgatt cagcgtcgca aaaagcgtct
      181 gtttaagtgg aaaggaaaag caacggaatt aaaaaaagaa cgggaccggg ttcagttctt
      241 cctctttcaa cgtttttttc aagaggcccg tcgccgcagg gacgtcgtag agcctccccg
      301 gagcgaagcg cgcccagaag tggcgaaggc ccggcacgac gaccttgatc acgggcattc
      361 cggtatcggg gcgggtctgg tccagcgcga gcatctccat ccccttcgcc tcgatcaggc
      421 ggcggcagta cgcgatgtcc tcaagaaagt cgtccgtatg ccgcaccggg tagtcggaga
      481 atgttcgcag aggcttcgac gggtccggcg ccatgtaggg cttgttggcg agcgtcgccg
      541 tacgcatcca ggagagcgtc tccttgtctt gcagtccggc gaggtcgtcc tcgcccccgc
      601 ccgagccgac ggagagcatc tggttcatct ccgcgaaggc tcgctggagc gcgatgcgtg
      661 cgtcgagatg gcaccccagt ccgaagaaga ggcgttcctc ccgttctccg atacggcgcg
      721 acactgcgac gaaggcgggg atgccgaggt cggaagtgat gtcgagcgcc catgtttccc
      781 gcccccatcg ctcccggtag tacgccgcaa gcttcacgat ccacggatcg ccgaagctct
      841 ccgcgtccac tccgggacgt gagagcatgt tataccacca gacggcgacg gcgtcgcgct
      901 ccaccagctc gaagaagcct tgcagcaagg cctcggcgag agtattgccg gaggcggcgc
      961 cgttggagca gccgatggag tagaccgtct cgtcgccgtc ccgcgccggg gcgcgatagt
     1021 ataacagttg cgtcggcagg tatttgtgcc gttcttcggt gagcgaccag acgggcgtcc
     1081 agtccacgat acgttccggg tcgaacggct ccggcaccct gttgaagcgg gagccgcggc
     1141 tgttccagac ctcccgttcg gcatactggc gttcgctgaa gcgcatgacg tcgttggggt
     1201 ggatcgcctc tccctcggac cagtcgttca gcgcgcgcgg aattcgcagt tcgccgcccg
     1261 tccactcgcc gctgtagcgt tcgacggcct cgcagagcgc gctcgccctg gcctgggcct
     1321 cggaggcgcc cttgcccgag ctggagctgc gcaggctgcg cttgagcgaa tccagccggt
     1381 ccatcttcag cgcggtgttg tgtccagcgg cgtagacgac cgcgacgtcg tcgtcgctgc
     1441 gcacgggcct cagttccttc accacccccg tgatcgggct gatcaggtgt tcatagcgtt
     1501 tcagcgtccg ttcgggggag actgcgcggc gccccccgtc cttgttgaat gtagctctcc
     1561 ccggttccaa cggcagcggt ttcgcctcgc ggggcgcctc cgtgccgcag gcggggcagt
     1621 aggggtggcg cacaagggcg tgggttcggg cgctccagtc ggtccagtcg aggctcagca
     1681 gcttccctgc aagcgtgcgg gacgctccgg cgaggaagcg cgccgcctcc gtcgccgcca
     1741 tgccgtacgc cgaggccagc gtcgacagga gagccgctac ggaggtcgag ggaactgcct
     1801 tcccgcctcc gagccttttc gccagagcgt gcgcgctgcg gttgcgttcg agtctcgaca
     1861 gaagacagcg gaagcaggcg gtctctccgg gcaggaagag cgggccgatc cacgcctccg
     1921 gccccgtggg acggagcagc agccaggggc gacgcgccgc gagcgcctcc ccgttgatct
     1981 cgcgcagctc gtcccgctgg tagtcgtccg cgacgacgac ccacaagtcc gccgactctt
     2041 tcggccccgt ccgcacgccc gcttctttca acgccgtctc cagcggcgaa ctgtccagat
     2101 tccccgcgac aaacacggcg acggaactcc tccgaagcgc ctccatcgcg gcgcgcggtt
     2161 cgacgcccgc ttcgtgccag aaagcggaca gggcgggagg tatttccgga gttgcctccg
     2221 cgacgcaccc ccgctgctcc aggatgttca agacgtagta gacagtcgct gcgtcgacgc
     2281 tgccggcgag cgcgtccgcg atgtcgtcgg cgctgcggca tccgtcgatc agcgggacga
     2341 caagttcgta ggcggcgccg taaaaggctt tcgcccccct ctcggagagc gcgagcgctc
     2401 cttcgccggg aaccgtttgc aggcggcagt gcggcgcgac cgccggggtc ttcatcgtgc
     2461 gctttccgaa cagtacggga tgaacaatct cccgtaccgg atgggagccg cgccggaagt
     2521 ttccgttacc acgttatgaa gcaattgttg tcatcccatg agtactgccc gcctgcggcg
     2581 gcgatgtcga gcgcctcgtc cgagagttcg tcgggttttt tcggaatcac aaggtgtatg
     2641 cgctcgtccg tattttcgaa gacgtcgatt tttacccctt ccggaatcgc cactccctcg
     2701 gccctcaaca ccgacgcggg atcggtcatc agcttcgcct tgaaccgagc gtccgcccac
     2761 gccttggcga tcagcttcgc caatactttg cgcatctctt ttctgtccat accgtacccc
     2821 tcccgttaaa taaagtagcg cttcggcgcg cgtccgccgc tcatgggcgg acgggcatac
     2881 catgaaggct cagtagaagt cgcacgagca gaagacgtcg ttctgcccct cggccgcggc
     2941 gatatccaac tcctcgtcag agagttggtc gggcttcttc gggataacaa ggtatatgcg
     3001 ctcgtccgtg ttttcgaaga cgtcgatccg cattccctcc ggaatcgaga atccttcggc
     3061 ccgcagaacc gacgcgggat cggccatcag cttcgccttg aactgcgcgt caacccacgc
     3121 cttggcgatc agctttcccg ttgccttgta catctctttt ctgtccattt tcgttcaccc
     3181 tccacactga aattttagaa atccgagcta ttctgaatta taaactctga catagtgtga
     3241 gagtcaaggc tgctttatgc tttttttcga aaaaatgata aagtggtgtc caaagaaaga
     3301 ccgcttcgat gaacacgctg cctctgcatt ggcgagcgag cggaacggac agacgaaaag
     3361 aaaaaagggc aagagaaaag ggtgaaataa tgaaaatgca cgaagaagcg cgccgcgcag
     3421 gcactgaaaa aaatgctccc gcaggggagg gagaaatatc cgccccgctc ttcgacccgg
     3481 aatctctcgc agtctctttg aagagaggcg tccgtctgca cgaaacggac ggacgcgccg
     3541 tacttgtcga cgcggacggc gttgcgctcg cccttccgct ttctcccgaa acgcggcgga
     3601 ttttcttgtc ggcgctggac gagggagtat gtaccgcgga cgagctgggg gacgcggcgc
     3661 ttcac
//
LOCUS       DAOSWW010000154         3656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17871_length_3656_cov_3.607331, whole genome shotgun sequence.
ACCESSION   DAOSWW010000154 DAOSWW010000000
VERSION     DAOSWW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17871_length_3656_cov_3.607331"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PK442_04370"
     CDS             complement(<1..440)
                     /locus_tag="PK442_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HOO86912.1"
                     /translation="MDEQRTRRHGAEFKTENGEEPVRKRDRDLSPSEIEEKSFRILEG
                     LLGDFQAPAPEREVMKRVAHATTDVEWAKTFRFSPNAVQAGIEAVRRGAPVVTDVEMV
                     SAGVRRSAAEARRFRNAQDGNETLCFLNDPDVAEEAKRSGTTRAR"
     regulatory      complement(642..757)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            932..1603
                     /locus_tag="PK442_04375"
     CDS             932..1603
                     /locus_tag="PK442_04375"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO86913.1"
                     /translation="MSLKETYKTKSGFVYELLRERIVAGEWPQGTVIVVASVARELGV
                     SAIPVREAMKQLQAEGLVELEPHRGARVTVFDPKKIVEVMSIRGALEGYAARVALPFI
                     DDAALADLRRKVDDMGTFTVAGDTARLVAANKEFHRKLYSMGPFPMLNDMIFKLWDGG
                     AFSSIVFALRPDRIRTVYEEHLAILAAIERRDAEELERLVRAHKSGFAEALCEISARK
                     RLETL"
     gene            1646..2413
                     /locus_tag="PK442_04380"
     CDS             1646..2413
                     /locus_tag="PK442_04380"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOO86914.1"
                     /translation="MKVFRYSVCAALIVCLCLALAVPVLADPAEMLLAKITGTNVNVR
                     AEPSRTGRLTGRFTGGEHAVVLEERSGADPFPWSRVIAFASGGTLVEGWVYGQYVEPL
                     PQDDWSNSAEGDPGERFSRFFDKTLERIGDTPEEAAARFGAPAAREDTRLPGRHDPAY
                     MVDYFRLEYPAFELLYFKTKDSYGLIGVKIVEGEAVIGEGIRLGADAASVVRELGVPL
                     YQKGSAFAWTDEPGYGVLTLTFAEGRVLNIDLDVELD"
     gene            2419..3123
                     /locus_tag="PK442_04385"
     CDS             2419..3123
                     /locus_tag="PK442_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO86915.1"
                     /translation="MAGRFFSGERVCVDTFFLEVFFAMRVQTSDVLKFLFLCVLFAAS
                     VSSPALCFDDSILPPLPEDELAQPHPQEDLPESGDVSGLDPSEVKTTGGVLAGVSSDI
                     FLPPLDADPEARARAVAIWNGAAELQRGKKYEEALEKYREGLAVCEDPVVREHIAKLE
                     AFLELQRKKAEASSARARAVALWNEAAELQLAKKYEEALEKYREGLAIASDPVVEEHV
                     KKLREFIEKRKARGKQ"
     gene            complement(3299..>3656)
                     /locus_tag="PK442_04390"
     CDS             complement(3299..>3656)
                     /locus_tag="PK442_04390"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO86916.1"
                     /translation="KGLGRPKDSMKAFYWFDRAARNGYIDAMVNLGVLYEKGDGAVFS
                     PEKAIRWYTEAAEKGSFLGMAYLGEAYYQGRGVRKDEKKAVGLLEQAASSGLPYAQRL
                     LYRIDRIKYMRYRDVQ"
BASE COUNT          698 a         1040 c         1116 g          802 t
ORIGIN      
        1 cgcgcacggg tcgttccgct ccgtttcgcc tcctcggcga cgtcgggatc gttcaggaag
       61 cagagcgttt catttccgtc ttgggcgttc cggaatcgcc gcgcttccgc tgcggaacgg
      121 cgaacgcccg cgctcaccat ctcgacgtcg gtcacgacgg gagcgccgcg ccgtacggcc
      181 tcgatgcccg cctgcactgc gttcggcgag aagcggaacg tcttcgccca ttcgacgtcg
      241 gtcgtggcgt gggcgactct cttcatcacc tcgcgctccg gagcgggagc ttgaaagtcg
      301 cccagcagtc cctcaagaat ccgaaagctc ttttcttcta tttccgaggg ggagagatcg
      361 cggtcgcgct tccggacagg ttcttccccg ttttctgttt tgaattccgc gccgtgccgc
      421 cgcgtcctct gttcgtccat ggaatctcct ccattcatac ggatacgcgg agcgcgcacc
      481 gctgaagaca aaaataaggc gaacccccgt gtgaggttcg ccggcgccgg tcatatatat
      541 gcgacgagtg caaaatccgg aaaaacctgt ccgtttgccg caaaaaatac atgaaaagcc
      601 gacccgaacc ctcgcaggtg tcgcacagat gcgcttcttc agggtcttct ggcttccttc
      661 atcctcgttc accccttccc ggcaaaaacc ggtggtcgat gaactcgtcg ggtacacagc
      721 ggcgggaccg cgccggaatt tcaccgggct tccccttgaa gacgagcgcg ttcatatagt
      781 attcgatgaa catctccatc ctaatcttcc ggtgtcgacc tgtcaatcgg atttcgtgga
      841 acgggtgcat ttcttcgttt gactaggtat aatatgcatc atatattgtg cttttaaaaa
      901 gggcttgatt tttcaaaatc aggaggtttc catgtcgctc aaagagacgt ataagacaaa
      961 gtcggggttc gtatacgaac tgctgcggga gagaattgtc gccggagagt ggccccaggg
     1021 aacggtgatc gtcgtcgctt cggtcgccag agagctcgga gtctcggcga tcccggtgcg
     1081 ggaggcgatg aagcagcttc aggccgaagg acttgtcgaa cttgagccgc accggggggc
     1141 gagagttact gtattcgatc cgaagaagat cgtcgaggtc atgtcgattc gcggcgcgct
     1201 cgaagggtat gccgcccggg tcgcccttcc attcatcgac gacgcggcgc ttgcggattt
     1261 gcgccgcaag gtggatgata tgggaacgtt tacggtcgcc ggcgatacgg ctcgtctggt
     1321 tgcggcgaac aaggagttcc atcggaagct ctattccatg ggccccttcc cgatgctgaa
     1381 cgacatgatc ttcaaactct gggacggggg cgccttttcg agcattgtct tcgctctcag
     1441 acccgaccgt atcaggacag tgtacgaaga gcaccttgca atccttgccg cgatagagcg
     1501 gcgcgacgca gaggaactgg aacgcctggt acgggcgcac aagagcggtt ttgccgaggc
     1561 gctttgtgag atttctgcga gaaagcggct cgagactttg tagcggcagt atccggtact
     1621 ttcatcccga aaggagttgg aattggtgaa ggtttttcga tattcagtat gtgcggcgtt
     1681 gattgtgtgc ctgtgtctgg cgcttgccgt gcccgtcttg gccgacccgg cggagatgct
     1741 cctggcgaaa attaccggga cgaatgtgaa tgtgcgagcc gaaccatccc ggacaggccg
     1801 tctcacgggg cgttttacag ggggcgagca tgcggttgtc ctcgaagaac gctccggcgc
     1861 ggaccccttc ccgtggtcgc gggtcatcgc attcgcttcc ggagggacgc tcgttgaggg
     1921 ctgggtctac ggccagtacg ttgaaccgct gccccaggac gactggagca atagcgccga
     1981 gggagatccc ggcgaacgct tttcacgttt ttttgataaa accctggaac gcatcgggga
     2041 tactccggag gaggcggccg ccaggttcgg cgctcctgct gcgcgtgagg ataccaggct
     2101 tcccggccgg cacgaccccg cctacatggt ggactatttc cgccttgagt acccggcctt
     2161 cgaacttctc tatttcaaga cgaaagacag ttacggtctg ataggagtga agatcgtcga
     2221 gggcgaggcc gtgatcggcg agggcattcg tctcggcgcc gacgccgctt ccgtcgtccg
     2281 ggaactcggc gtcccgttgt atcagaaagg ctctgccttc gcatggaccg acgagcccgg
     2341 gtacggcgtc ctcacactta ccttcgcgga ggggcgcgtt ctcaatatcg acctggacgt
     2401 cgaactggat taggaggcat ggcggggcgc tttttctcgg gagagcgcgt atgcgtcgat
     2461 actttttttc tggaggtgtt ttttgccatg agagtacaga cctccgatgt tctgaagttt
     2521 ttgttcctgt gcgtgctgtt tgcggcgagc gtatcttctc ctgcgctctg cttcgacgac
     2581 agtattttac cgccgcttcc cgaggatgaa ctcgctcagc ctcatcctca ggaagacctc
     2641 cctgagagcg gcgatgtttc cggactcgac ccttccgagg tgaagacgac gggaggcgtt
     2701 ctcgcaggag tatcgtcgga cattttttta cccccgctgg acgcggaccc ggaagcccgt
     2761 gctcgcgccg ttgcaatttg gaacggcgct gcggagttgc agcgtgggaa aaagtacgaa
     2821 gaggcgctgg agaagtaccg cgaggggctt gcagtgtgcg aggatccagt tgtccgcgag
     2881 cacatagcaa agctggaggc atttcttgag cttcagcgga aaaaggcgga agcctcctct
     2941 gcacgtgcgc gcgccgttgc gttgtggaac gaagccgctg aattgcagct cgcaaagaag
     3001 tacgaagagg cgctggagaa gtaccgcgag ggactcgcca tagcatccga ccctgtcgtc
     3061 gaggagcatg tcaagaagct ccgggagttc atcgagaagc ggaaggcccg cggaaagcag
     3121 taaacaagga gcgccccgat gaaacaattt tatcgaatgc tctccgaaac gccgctccgg
     3181 cggaagtgtt ccgtgtgcgg aagaagagac ctcatcccga tgaaggggat gaggtctcta
     3241 taactatggt cgggcgccga aagcctgccg cacttccggg gacagcgtgt atgtcgggct
     3301 attgaacgtc gcggtaccgc atgtacttga tcctgtcgat ccggtagagc agtctctgcg
     3361 cgtagggcag ccccgacgag gccgcctgtt cgaggagccc caccgctttc ttttcgtctt
     3421 ttctgacgcc cctgccctgg tagtaggcct ctccgaggta cgccatacca aggaaagacc
     3481 ccttctccgc cgcttccgtg taccagcgga tcgctttctc cgggctgaag acggcgccgt
     3541 cgcctttctc gtagagcacc cctagattga ccatggcgtc gatgtacccg ttccgggcag
     3601 ctctgtcgaa ccagtagaag gccttcatcg agtccttggg gcgtccaagc cccttg
//
LOCUS       DAOSWW010000155         3647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17935_length_3647_cov_3.769766, whole genome shotgun sequence.
ACCESSION   DAOSWW010000155 DAOSWW010000000
VERSION     DAOSWW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17935_length_3647_cov_3.769766"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PK442_04395"
     CDS             complement(<1..631)
                     /locus_tag="PK442_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO86917.1"
                     /translation="MLHHLAGKRIWICTLGCRSNQYEGEALANAFTEAEAILSDTPDC
                     DAAVLVSCTVTSVADKKCRQMIGRVRRTSPGAIIVACGCWAQHLPEEEAQSLGIHLLV
                     GNRKKQHVPLLLAQLFGDNSRRFSVCREDVLHSTEWDSLFLAKPLLHTRAFVKVQDGC
                     IHFCSYCAIPYARGFPVSRSPDDVLEEVRSIVSSGCREVVLTGVHLGLYG"
     gene            complement(609..1496)
                     /locus_tag="PK442_04400"
     CDS             complement(609..1496)
                     /locus_tag="PK442_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOO86918.1"
                     /translation="MNSSAESFWWYVTLEGRPGSEEVLASLADMSGSIGSEETAGEAS
                     VLLRAYFRSSQDLGFWLERLGGALSPWPEVRIADMGKIENKQWHTDWKEAFPPLAVGE
                     GLVVLAPWHKGNEPEGRMPLYIYPGSAFGTGYHESTQIVLTLLERTVVPGITAADIGA
                     GSGILSIAAVKLGAGHVRARDLDPAVLSEISHNLGLNEIPSGTVDLEEGNLLDGMDET
                     VDLLMANIIIEPLLEMLPNVPARLKEKGRALFSGLLVKERERFCAALEATGLRVIDEL
                     SLGDWWGVTVEPYAAPSRR"
     gene            complement(1634..2779)
                     /gene="dnaJ"
                     /locus_tag="PK442_04405"
     CDS             complement(1634..2779)
                     /gene="dnaJ"
                     /locus_tag="PK442_04405"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOO86919.1"
                     /translation="MYSGGETDDLYQILGVPRDASAADIKKAYRQLVRKYHPDANPGN
                     KDAEERFKKINMAYEVLSDSQKRTQYDQFGTVGDMPGGGSPFEGFGGMGDIFGDIFDS
                     VFGGGMGRRRANPNAPRRGADLEMEVEVTLLEAATGAAKEFQIPRWESCGACGGTGAK
                     AGTSPESCSQCGGRGQVESRQQTPFGQFVSVNACPRCNGTGKIIKEKCPECGGQGRVR
                     TQHKVEVKIPAGVDTGTRLRISGEGEPGVNGGTSGDLYLVVSVQEHPDFKREGGDLHT
                     RLSISFPQAALGCTVDVPVLSGEPETLDIPAGTQPGKVFTLRGRGVPRLRGPRGKGDL
                     FVHVTVDVPTKLTEKQRALLEELAREMNVTVEDSGVFGKFFRNLFEG"
     gene            complement(2795..>3647)
                     /locus_tag="PK442_04410"
     CDS             complement(2795..>3647)
                     /locus_tag="PK442_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO86920.1"
                     /translation="KIKELLGKDPTKGINPDECVAAGAAIQGAILAGDHKDIVLVDVT
                     PLSLGLETLGGVFTKIIERNTAIPVSKSQTFTTAGNNQTQVEISVLQGERAMAADNVK
                     LGKFVLDGIPPAPRGIPQIEVTFNIDVNGILNVSAKDKGTGKAQNISIHSSNLSKDDI
                     ERMKNQAEEFEEEDKKKRETAETKNEADGAAYAAERLLSENGDKMTPEEKGKITSGME
                     ELRRLLSGDDLASIRSAKESLEKALQEFSTRLYSQAQQQGDSSGTAGSASDGDTVDAE
                     FSDQGQA"
BASE COUNT          707 a         1170 c          985 g          785 t
ORIGIN      
        1 ctccgtacag tcccagatgc accccggtga gcacaacctc ccggcagccg gaagagacaa
       61 tagacctaac ctcctccaga acgtcgtccg gagagcggct gacgggaaat cctcgagcat
      121 agggaattgc acagtaagaa cagaaatgaa tgcagccgtc ctgcaccttg acgaaggcgc
      181 gggtatgaag cagcggcttc gcgaggaaaa gagaatccca ctccgtactg tggaggacat
      241 cctcccggca taccgaaaaa cgccgggaat tatccccgaa gagttgtgca agaagaagcg
      301 gaacatgctg ttttttccgg ttgccgacga gcagatgaat cccgagcgac tgcgcctctt
      361 cctccggaag atgctgcgcc cagcacccgc acgcgacgat aatcgcgccg ggggacgttc
      421 ggcgtacccg cccgatcatc tgccggcatt ttttatccgc aaccgaagtg acggtacagc
      481 tcaccagaac cgccgcatcg cagtcggggg tgtcgctcag aattgcttcc gcctcggtga
      541 aggcattggc aagagcctcg ccctcgtatt ggttggaccg gcatccaagc gtgcagatcc
      601 agattctctt accggcgaga tggtgcagca tagggttcta ccgttacccc ccaccagtcg
      661 ccaagtgaga gttcgtcgat aacgcgcaat cctgttgcct caagcgcagc gcagaaacgc
      721 tcacgctcct ttacaagcaa acctgaaaaa agagcgcgcc ccttctcctt cagtcgtgcg
      781 gggacattcg gaagcatttc gaggagcggt tcgataataa tattcgccat gaggaggtcg
      841 acggtttcgt ccattccgtc gagaagattc ccctcctcaa ggtccaccgt tccggagggg
      901 atttcgttca gtcccagatt atgactgatt tcggagagca cagcgggatc gaggtcccgc
      961 gccctgacat gtccggctcc cagcttgacc gcggcaatgg agaggatgcc ggaacctgct
     1021 ccgatgtcgg cggcagtgat tccggggacg acagtacgtt ccagaagcgt cagcacaatt
     1081 tgcgtgctct cgtgataccc ggtgccaaac gcggacccgg gatagatata taagggcatc
     1141 cggccttcgg gttcgttccc tttgtgccag ggagccagaa cgacgagccc ttctcctact
     1201 gcaagaggag ggaaggcttc cttccagtcg gtatgccact gtttgttctc tattttcccc
     1261 atatcggcga tcctgacttc cggccacggg gacagcgccc cgccaaggcg ttcgagccaa
     1321 aaacccagat cctgggagct gcgaaagtat gcccgcagga ggacggaagc ttctccggca
     1381 gtctcctctg aaccaatgct tcccgacata tctgcaagag aggccagaac ctcctcgctc
     1441 ccgggccgcc cttccagagt gacataccac cagaagcttt ctgctgaact gttcatgccg
     1501 aattcctccc ggaagatgaa ataagggagg ctagtgcagc ctcccttttc gtgctgataa
     1561 agagtgtagc agaagaagcg ccgtttttac agggcgaata ctcttttact gaacgagcgt
     1621 ctcgaaaaaa aacttaccct tcgaagagat tcctgaaaaa tttcccgaaa accccggaat
     1681 cctccaccgt gacattcatc tccctggcca gctcctcaag aagagcgcgc tgcttctccg
     1741 tcagcttcgt cggcacatcc accgttacat ggacaaacag atctcctttg cctctgggac
     1801 cgcgaaggcg cggaacgccc ctgccgcgga gagtgaatac ctttcccggc tgtgttccgg
     1861 cagggatgtc cagcgtctca ggctcgccgg aaagcacggg tacatcaact gtgcacccca
     1921 gagcggcctg agggaaagat atggaaagac gggtatgcag gtctccgccc tccctcttga
     1981 aatcggggtg ctcctgaacg ctcacgacga gatagagatc gcccgaggtt cctccgttta
     2041 cccccggctc gccctctccg gatattcgta gcctggttcc ggtatccacg cccgccggaa
     2101 ttttcacctc gaccttgtgc tgcgtgcgaa cccttccctg gcctccgcac tccggacact
     2161 tctccttaat gatctttccc gtgccgttgc accggggaca ggcattcacc gagacaaact
     2221 ggccgaaagg cgtctgctgc ctgctctcca cctgtccgcg cccgccgcac tgtgagcagc
     2281 tctcgggcga cgtaccggcc ttcgcccctg tcccgccgca cgcgccgcac gattcccagc
     2341 gcggaatctg aaattctttc gccgccccgg tcgccgcctc caacagcgtg acttcgacct
     2401 ccatctccag gtcggccccc cgtcggggag cgtttgggtt cgctcttctc cgccccattc
     2461 caccgccgaa gacgctgtcg aatatatcgc cgaagatgtc ccccataccg ccgaagcctt
     2521 cgaacggact ccctccgccc ggcatatcgc ccacggttcc gaactgatcg tactgggtcc
     2581 gcttctggga gtcgctcagc acttcgtacg ccatattgat cttcttgaaa cgctcctcgg
     2641 catctttgtt gcccggattg gcgtcggggt ggtactttcg taccagctgg cggtaggctt
     2701 tctttatgtc agcggcggaa gcgtcccggg gcacccccag tatttgatac aggtcgtcgg
     2761 tctctcctcc gctgtacata cacggtcacc tcccctacgc ctggccctgg tcgctgaact
     2821 cagcgtccac ggtgtcgccg tcggaggcgc tccccgccgt tccggaagag tcgccctgct
     2881 gctgcgcctg agaatagagc ctggtggaga actcctggag cgccttttcg aggctctcct
     2941 tcgccgagcg gatggaggcg agatcatcac cggagagaag cctgcggagc tcctccattc
     3001 ccgacgtgat tttccccttc tcttcagggg tcatcttgtc tccgttctcc gagagaaggc
     3061 gctccgccgc gtatgcggcc ccgtccgctt cgttcttcgt ctcggccgtc tcacgcttct
     3121 tcttgtcctc ttcttcaaac tcctccgcct gattcttcat ccgctcgatg tcgtccttcg
     3181 agaggttcga cgagtggatg gagatattct gcgccttgcc cgtccccttg tccttcgcgc
     3241 tcacattgag aatgccgttg acgtcgatat tgaaggtgac ctcgatctgc ggaatgccgc
     3301 ggggcgccgg gggaatcccg tcgaggacga atttgcccag cttcacgtta tcggcggcca
     3361 tggctcgttc tccctggagc acgctgatct cgacctgggt ctgattgttc cccgcagtgg
     3421 tgaacgtctg gctcttcgag acagggatgg cggtgttgcg ttcgatgatc ttcgtgaaca
     3481 cgccgccgag cgtctccaat ccgagggaga gaggcgttac gtcaacgaga acaatatcct
     3541 tgtggtcgcc cgcgagaatg gctccctgaa ttgcagcccc cgccgcaacg cactcgtcgg
     3601 ggttgatacc ctttgtagga tccttcccga gaagctcttt aatcttc
//
LOCUS       DAOSWW010000156         3646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17944_length_3646_cov_3.139794, whole genome shotgun sequence.
ACCESSION   DAOSWW010000156 DAOSWW010000000
VERSION     DAOSWW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17944_length_3646_cov_3.139794"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PK442_04415"
     CDS             complement(<1..1088)
                     /locus_tag="PK442_04415"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HOO86921.1"
                     /translation="MNYTVMQFGEGNFIRSFFDWMLKRITDATGEEHKVFLVQPISNG
                     RVKEIVEAGEYHVLLKGFEAGEYKEVLESVPVIAGGVNPFERGGMKALLDAALSPELR
                     VVTSNTTEAGIFFEEKMEPHNFPSLLAAVLRARAKEGAPPLHILPLELIESNGTALKK
                     CVEQYGRLWGCEPVFFSYLDSCTFYDTLVDRIVPGFPAKDAPEIFRRLGKEDHNLAMG
                     ELFHLFVIQGDKAILDVLPFHKAGLNVIVTADKLPFYRDRKVRILNGVHTASVPVGLL
                     AGVELVKDFVKDERFAPMLRRLVHDEIVPAFSDDQEAHRYGDDVLERFSNPALEHAFR
                     SIALNSVAKSNTRLRPTLEGYFEKFGELP"
     gene            complement(1164..2654)
                     /locus_tag="PK442_04420"
     CDS             complement(1164..2654)
                     /locus_tag="PK442_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HOO86922.1"
                     /translation="MECTHVGARCIVLRENDSVAVLPEGGGAGSRLLFAEQELVLKEV
                     IAPGHKIALRDIKQGERVVKYGFPIGVASRPIAAGEHVHVQNVRSALGHEWSPSWSPS
                     PARLLSPLSPVPSFQGYRRTEGPPGIRNELWIIPTVGCVNEYLKFLAGAWTAPSWIEN
                     VRVLSHPYGCSQLGEDLERTAAVLAGLARNPCAAGVIVAGLGCENLQRVFLETRLAGV
                     EKVRFLSLQEEDDDAGALYALLDELAADAPKEREPFPLSGVTLGVKCGGSDGFSGLSA
                     NPLVGEVADRMSAWGGKILATEIPEMFGAEDVIASRIADRQVFDAFAETIRWFRNYYV
                     EHKQPVYENPSPGNKDGGITTLEEKSLGAVNKCGSGAVTDVLAMGEQAKRPGVSVVFG
                     PGNDLVSSTALAAGGAQIILFTTGRGTPFSTVVPTIKISSNTRLFGRKPRWIDFDAGP
                     LLSGTPRHLLAEALTGLVLEVASGRRTRNEENRSGEIAIFKDGVTL"
     gene            complement(2849..>3646)
                     /locus_tag="PK442_04425"
     CDS             complement(2849..>3646)
                     /locus_tag="PK442_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOO86923.1"
                     /translation="RQIANAHTIFNVTNTLLFLPFVPVLVWVIRRIVPDKGGAVVAGP
                     MYLDLKLISASPAAAVDAVKKELLHMGDLARSMLTDVRRAFTENNPKMIGEVTQTEKA
                     VNEINRAISSYASEVWQGGLSSELSTVLGSYVNGVGDIERIGDHATNLIELYEYKLDH
                     GVEFSTLARGEFDDMFDLVERAVRLSFESLNEEDVIKTKEVDALEDEVDRKEKILRKN
                     HILRLNKGECTPAGGVIFIDILSNLERVCDHAHNISLIAFDISKLHR"
BASE COUNT          746 a         1120 c         1045 g          735 t
ORIGIN      
        1 ggcagctcgc cgaacttctc gaagtacccc tccagcgtag ggcggaggcg ggtgttgctc
       61 ttcgcgacgg agttcagcgc gatggagcgg aaggcatgtt ccagcgcggg gttgctgaag
      121 cgctcaagga cgtcgtcgcc gtagcggtgc gcctcctggt cgtcggagaa tgcaggaaca
      181 atctcgtcat gaaccagccg gcgcagcatc ggcgcaaagc gttcgtcctt cacaaagtct
      241 ttgacgagtt ccaccccggc gagaagaccg actggaacgg acgccgtgtg caccccgttg
      301 agaatccgca cctttcgatc tctgtagaac ggaagtttgt cggcggtgac gatcacattc
      361 aatcccgcct tgtggaaggg caggacatcg agaatcgcct tgtccccctg gatcacgaac
      421 agatgaaaga gctcccccat ggcgaggttg tgatcctctt tccccagacg ccgaaagatt
      481 tcgggggcgt ccttggcggg gaagcccggg acgatacggt ccaccagcgt atcgtagaag
      541 gtacaggagt cgagatagga gaagaatacc ggttcgcacc cccagagacg tccgtactgc
      601 tccacgcact ttttcaaagc cgttccgtta ctctcgataa gctccaacgg aaggatgtgc
      661 agcggaggcg ccccctcctt tgcgcgggcg cgcagaactg cggccagcaa cgacggaaag
      721 ttatgcggct ccatcttctc ctcgaagaag atcccggcct cggtggtatt cgacgtcacc
      781 acccgaagct ccggagaaag cgcggcgtcg agcaacgctt tcattccgcc gcgttcgaag
      841 gggttgacgc cccccgcgat gaccgggacg gactcaagaa cctctttata ctcccccgcc
      901 tcgaatcctt tcagaagaac gtggtattct cccgcctcga cgatctcttt cactcttccg
      961 ttggaaatag gctgcacaag aaagaccttg tgctcttcgc ccgtcgcgtc ggtaatgcgc
     1021 ttgagcatcc agtcgaaaaa cgaccgtatg aagttcccct cgccgaactg catcactgta
     1081 tagttcatcg tatcctctcc ttcgtcgcga gcatttctgt gcatcttggt ttcgcacggc
     1141 gtcctcgttg aaatcggggc ctcttagagc gtcacgccgt ctttgaatat ggcgatctcc
     1201 ccggagcggt tctcctcgtt tcgggtacgg cgccccgacg caacttcaag cacaagcccc
     1261 gtcaaggctt cagcgagaag gtgcctcggc gtcccggaga gcagcggccc ggcgtcgaag
     1321 tcgatccagc gcggtttccg cccgaaaagg cgcgtgttcg aggaaatctt tatcgtcgga
     1381 actaccgttg agaacggcgt cccgcgtccc gtagtgaaaa ggatgatctg tgcgcccccg
     1441 gcggccagag cggtcgacga cacaaggtcg tttcccgggc cgaacacgac gctcacaccg
     1501 gggcgcttcg cctgttcgcc catggcgagg acgtctgtta ccgcaccgga accgcacttg
     1561 ttcaccgcgc cgagcgactt ctcctccaac gtagtgatgc cgccgtcctt gttccccgga
     1621 gacgggttct cgtacaccgg ctgcttgtgt tccacatagt agttccggaa ccagcgaatg
     1681 gtctccgcaa aggcatcgaa gacctgcctg tcggctattc gagaggcgat aacatcctcg
     1741 gcgccgaaca tctcaggtat ttcggtggcg aggatcttcc ccccccacgc gctcattctg
     1801 tcggcgactt cgccgacgag aggattggct gaaagaccgg agaagccgtc gctgccgccg
     1861 cacttcacac cgagcgtcac cccggagagg gggaatggtt ctctctcttt cggagcatcc
     1921 gccgcaagct catcgaggag agcgtacaag gctccggcat cgtcgtcctc ctcctggagc
     1981 gagaggaatc ggactttttc cactcctgca aggcgggtct ccagaaagac gcgctggagg
     2041 ttttcacacc cgagccccgc gacaatcacc ccggccgcgc acggattgcg cgcaagcccg
     2101 gcaaggacgg ctgccgtccg ttccaaatcc tccccgagct gggagcagcc gtaggggtgg
     2161 gaaagcacgc ggacgttttc gatccaggaa ggggccgtcc acgctccggc gaggaatttc
     2221 aggtactcgt tgacgcaccc gaccgtcgga atgatccaga gctcgttccg tatgcccgga
     2281 ggcccctccg ttcttcggta tccttgaaat gaaggaacgg gcgatagcgg ggagagaagg
     2341 cgcgcaggag aagggctcca cgacggcgac cattcgtgtc cgagagcgga acggacgttc
     2401 tgcacatgca catgctcgcc tgcggcgatc ggacggctcg caacgccgat cggaaaaccg
     2461 tacttcacca cccgctcccc ctgcttgatg tcccggagag caatcttgtg tccgggagcg
     2521 ataacctctt taagaacgag ctcctgctcg gcaaagagaa gccgcgatcc ggcgcctcct
     2581 ccctcgggaa gaaccgcgac gctgtcgttc tcccggagga cgatacagcg cgctccgacg
     2641 tgtgtgcatt ccatagcctc tcctccttgt tttatccatt aaaacaaaaa tttaccgagt
     2701 aaaacaagaa ccgtctacag aatagcgaat gcacccggta tgtcaaggag ggagcatgaa
     2761 aaaactcgtt caaaaaaccg gcgaggagcc tccccgccgg tttcaagaca taccaatatt
     2821 cgtccacctc aagaagcccg gtcgtccgtc atctgtgcag ctttgatata tcgaacgcga
     2881 tgagcgagat attgtgggcg tgatcgcaca ctcgttcgag attgctcagg atgtcgatga
     2941 agatgacgcc cccggcggga gtgcactccc ctttgttgag acggaggata tggtttttcc
     3001 ggagaatttt ctccttgcgg tccacctcgt cctcaagggc gtcgacctcc ttggtcttga
     3061 tgacgtcctc ctcgttgagc gactcgaaac tcagacgaac cgcccgttca acgaggtcga
     3121 acatgtcgtc gaactcccct cgggcgagcg tggagaactc aacgccgtga tcaagcttgt
     3181 attcatagag ctcgatcagg ttggtcgcat gatccccgat gcgttctatg tcgccgacgc
     3241 cgttgacgta cgagccgagt accgtcgaaa gttcgctcga aagccctccc tgccagactt
     3301 ccgacgcgta ggaggagatg gcgcggttga tctcgttcac ggctttctcc gtctgtgtca
     3361 cctcgccgat catcttcggg ttgttctccg tgaaggccct gcgaacatct gtcagcatcg
     3421 aacgggcaag atcccccatg tggaggagct ccttctttac cgcgtccact gcggctgcgg
     3481 gagatgccga tatgagcttc aagtccaggt acatcggacc cgcaacgacg gcgcccccct
     3541 tgtcggggac gatccggcgt atgacccaaa caagaacagg tacgaaggga aggaagagca
     3601 gtgtgttcgt cacgttgaag atggtgtgcg cgttggctat ctgccg
//
LOCUS       DAOSWW010000157         3644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_17954_length_3644_cov_4.028142, whole genome shotgun sequence.
ACCESSION   DAOSWW010000157 DAOSWW010000000
VERSION     DAOSWW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17954_length_3644_cov_4.028142"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PK442_04430"
     CDS             complement(<1..1296)
                     /locus_tag="PK442_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO86924.1"
                     /translation="MSKSIVAKLGLLVAASMILLAASLAFLAQRGVDQMSRSVEQVAE
                     RMLNEDIKNKNANDERSAETYGKAMSGYLASIAATPLWDFDEESLQNYAKDMLNVPNV
                     AYAAIFAADGAPMAGEKKEAAHIKAFTSDILREGKTIGSVEIGLDTTYLAELTKASEQ
                     TRDQLIASFSAEASDAESAIVRRILLFAIGIVAAVLLLNVIGLLRVVAPLRLMTDVVR
                     DLGEGEGDLTVSIAVKTKDEVGRLGMSMNQFIEKLARLVIDVIGIARRVGGETRMLSD
                     LSGKSMDSIELVNGAVEQIVSLSETNAAAVEETNAGVTEMSATAAAVAEAAGQCVQSS
                     AKTSEFTKDVARRMEEVANDIDAVSLRSQENRTKMSALAEAVDSIAGFVGAITGFADQ
                     TNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEESNRAA"
     gene            1655..2269
                     /locus_tag="PK442_04435"
     CDS             1655..2269
                     /locus_tag="PK442_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO86925.1"
                     /translation="MAGNGKHLPAEERRAVIVETVLELAAEGNPAEITTAAIAERMRL
                     TQGALFRHFQSKDAVWSAVMEWVAERLLLLVSRAMKGASTPLAALEAAFTANVRFAAE
                     HPGAPRLLFSELQRTGESVPRDIARALMKRYAERLRLIVEEGKERGEIRNDVSSEASA
                     HLFIGVVQGLVLQSLLAGDAQAILRLAPEAFAIFRRGIEEAGRS"
     gene            2266..>3644
                     /locus_tag="PK442_04440"
     CDS             2266..>3644
                     /locus_tag="PK442_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO86926.1"
                     /translation="MRRGSMKGRTLLLVLLLLPLAGLFVYVMTNSGPLAPVPVTVAEV
                     ESRSISPALYGIGVVESRAVHRIGATSPGRLLWLDAQVGEMVAAGQVLGELDPVDLND
                     RIEGQKAAVRRLESLVAAADSRVAETKARKEYAEAQATRYENLLKKKAVSVEAADAKR
                     QERRTTAAAWSSARADAEAARAEQARAGSELKGLLARKEDLKLVSPVQGLLAAREAEP
                     GSTVVAGQSVFQVVETEGLRISVRFDQSMASGLRAGLPAEIVLRSRADEVFAGNILRV
                     EPIADAITEELVAKAVFEQTPGTLPPLGELAEVTVRLPALPEGPVVPDAALRRFDGRL
                     GVWMMENGSISFRPVVAGRRDLNGMVRILEGLEPGERVAIVGHTGAGKTTIINLLSRF
                     YDIQQGAILVDGIDVRDYEKTSLRRQIGIVLQDVFLFSGSVRNNIGLGNSSLSEERMK
                     ACSEYVNAAP"
BASE COUNT          671 a         1049 c         1180 g          744 t
ORIGIN      
        1 cgcggcgcga ttggactcct cggcgagctt ccggacctcg tcggcgacca ccgcgaaacc
       61 gcgtcccgcc tctcccgcgc gggcggcctc gatggccgcg ttgagggcca gcagattggt
      121 ctggtcggca aaaccggtaa tggcgccgac gaaccctgcg atcgagtcca ccgcttctgc
      181 aagcgcggac atcttcgtcc tgttctcctg agagcggagg ctcacggcgt cgatgtcgtt
      241 cgccacttcc tccattctcc gggcgacgtc cttcgtgaac tcggacgtct tcgcggagga
      301 ttgcacgcac tgtcccgcag cctcggcgac cgcggctgcg gtggcggaca tttcggtgac
      361 gcccgcattc gtctcttcga ctgcggccgc gttcgtctcc gagagcgaga cgatctgctc
      421 caccgcgccg ttgacgagtt caatggagtc catcgacttt ccggagaggt ccgagagcat
      481 gcgggtttcg cccccgaccc tgcgggctat tccgatcacg tcgatcacca ggcgggcgag
      541 tttctcaatg aactgattca tggacatgcc cagtcgtccc acttcatctt tcgtcttgac
      601 ggcgatcgag acggtaaggt cgccctctcc ctctccgagg tcgcggacca cgtcggtcat
      661 caagcgcagc ggggcgacca cccgaaggag tccgatgaca ttgaggagga gcaccgcggc
      721 gacgatgccg atggcgaaga ggaggatgcg cctgacgata gcgctttcgg cgtcggatgc
      781 ttcggccgag aaggaggcga tgagctggtc cctggtctgt tcgctggcct tcgtgagttc
      841 cgcaaggtag gtcgtgtcga gtcctatctc caccgaacct atcgtcttcc cttcacgcag
      901 gatgtcggag gtgaacgcct tgatgtgcgc cgcctccttc ttctcgccgg ccatgggagc
      961 gccgtctgcg gcgaagatgg ccgcataggc gacgttcggt acgttgagca tatccttcgc
     1021 gtagttctgg agcgactcct cgtcgaagtc ccaaagaggc gtggcggcta tggaggcaag
     1081 atagccggac atggcctttc cataggtctc ggcgcttcgt tcatcgttcg cgttcttgtt
     1141 cttgatgtcc tcgttgagca tccgttcggc aacctgttca accgaacgcg acatctggtc
     1201 cactcctctt tgcgccagga aggcgagcga agcggccaga agaatcatgc tcgccgccac
     1261 cagtaaaccg agctttgcca cgatgctttt cgacacacga attaccccct cgagaatttt
     1321 tgacgcgacg gaaagtgtcc gacggcatcg aacgtgctgc gggaaaaggt attcccgggc
     1381 agggaatacg tcttggcgta ctttgagaga aaggaaaaag aaattgtaat gagctttgta
     1441 tccttctatg agagttgtac caagtacttc ccccaattta aagaatacac ttaaattctc
     1501 tttgctgcaa gctttgtctt tgcctcgttc cgctttttct ccgatgtaca gtaaatatgg
     1561 agcaggcaga gcgtgtcagg ggcttgacag aatgaatgcc ggcgcataag atagtaatca
     1621 attacttact tttgaaacga gagaggggat cagcatggcg ggcaacggaa aacatcttcc
     1681 tgccgaggaa cggcgcgcgg ttatcgtcga aacggtgctt gaactggcgg ccgagggcaa
     1741 tcccgccgag atcaccactg cggccatcgc cgagaggatg cggctgacgc agggggcgct
     1801 gtttcgccat tttcagagca aggatgctgt gtggagcgca gtcatggagt gggtagcgga
     1861 gcgcctgctt ctcttggttt cgcgggcgat gaaaggcgcg tcaacgcctc ttgccgccct
     1921 ggaggccgcc ttcactgcga atgttcgctt cgccgcggag catccgggag cgccgaggct
     1981 gctcttcagc gagctgcagc gcacgggcga gagcgttccc cgcgatatcg ccagggcgct
     2041 gatgaaacgg tacgccgagc gtctccggct gatcgtcgaa gaggggaagg agcgagggga
     2101 gatccgtaat gacgtctctt ccgaagcctc ggcgcatctc tttatcggcg tcgtccaggg
     2161 gcttgtgctc cagtcgcttc tcgcggggga cgcgcaggcg atactccggc ttgctcccga
     2221 agcgtttgcg attttccgca gaggcataga ggaggcggga cgctcatgag aagaggatcg
     2281 atgaagggac ggacgttgct gcttgttctg ctgctgcttc ctcttgcggg gctctttgtc
     2341 tatgtgatga cgaactccgg gcctcttgcc ccggtccccg tgacggtggc cgaggtcgaa
     2401 agcagatcga tctccccggc gctctacggt atcggcgtcg tggagtcccg tgcggtgcac
     2461 agaataggcg cgacatctcc ggggaggctg ctctggcttg atgcgcaggt cggagaaatg
     2521 gttgcagcgg gccaggtctt gggtgagctg gatccggtgg acctgaacga ccggatagag
     2581 gggcagaaag cagccgtacg gcggctggag tctctcgtag cggcggctga ttcccgggtg
     2641 gccgagacga aagcgaggaa agagtacgcc gaggcgcagg ccacacgcta cgagaacttg
     2701 ttgaagaaaa aggcggtaag cgtcgaggct gccgacgcca agcgccagga gcggcggacg
     2761 accgccgccg cgtggtcgtc cgcgcgcgcc gatgcggagg ccgcccgggc ggagcaggcg
     2821 cgtgccggat ccgagctgaa ggggctgctc gcccgcaagg aagatctgaa gctcgtgtcg
     2881 cctgtacaag gccttctggc ggcccgcgaa gcggaaccgg ggagtacggt tgttgcagga
     2941 cagagcgtgt ttcaagtagt ggaaaccgag ggcctcagga tctccgtccg gttcgaccag
     3001 tcgatggctt ccggactgcg ggctggactt cctgcggaga tcgttctccg ctcccgtgcg
     3061 gacgaggtct ttgccgggaa tatcctccgc gtcgagccga tcgcggacgc gatcaccgag
     3121 gagctggtgg cgaaggctgt tttcgagcag actcccggaa cgctgccccc tctcggcgag
     3181 ctggccgagg tgacggtgcg cctccccgcg ctgccggaag ggcctgtggt ccccgacgcg
     3241 gcgctccggc gtttcgacgg acgcctcggc gtctggatga tggagaacgg ctccatctcg
     3301 ttccgtccgg tggttgccgg tcggcgcgac ttgaacggca tggtgcgcat tcttgaaggg
     3361 ctcgaaccgg gagagcgcgt cgccatcgtg ggtcacaccg gcgcgggaaa aaccaccatc
     3421 atcaatctgc tcagccgttt ttacgatata cagcaaggag ccatattggt ggacggtatc
     3481 gatgttcgcg actatgagaa aacctccttg cgtcggcaaa tcggcattgt gttgcaagac
     3541 gtatttctct tttccggttc ggttcgtaac aacataggtc tgggaaattc atcgttaagc
     3601 gaagaacgga tgaaggcctg ctccgagtac gtgaatgcag cgcc
//
LOCUS       DAOSWW010000158         3637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18016_length_3637_cov_3.865159, whole genome shotgun sequence.
ACCESSION   DAOSWW010000158 DAOSWW010000000
VERSION     DAOSWW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18016_length_3637_cov_3.865159"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PK442_04445"
     CDS             <1..1276
                     /locus_tag="PK442_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOO86927.1"
                     /translation="SMLLNLSGAHGPYFTRNVVLLTDSEGRTGVGEVPGGEKIAGGLE
                     QARDLVVGSRTGEYKNTLLAVKKLFAGREDDVRGQQTFDLRTGVHVLTGIEAPLLDLL
                     GQYLGVPAASLLGDGQLRERIPVLGYLFYVADRKKTDLPYISESGSSVEWYRLRREEA
                     TTPEAIVALAKASRDLYGFSDFKLKGGVFPGSEEMEAVRTLKRAFPDGRITIDPNGGW
                     LLKEAVELCRGMHGILSYCEDPCGAEGGFSGREVMAEFRRATGLPTATNMIATDWRQM
                     AHSAMLQSVDIPLADPHFWTMEGSVRVGQFCEVFGLTWGSHSNNHFDISLAMFSHTAA
                     AVPGRITAVDTHWIWQEGTERLTKEPLTIRDGFIDLPAAPGLGIEPDMEAIEAAHRLY
                     MEHCLGARDDAAAMQFLIPGWKFDPKRPCMVR"
     gene            1334..2353
                     /locus_tag="PK442_04450"
     CDS             1334..2353
                     /locus_tag="PK442_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOO86928.1"
                     /translation="MKFRKALLALLVLALLAVPAVAGANPFAGMNVRVVIGSTSTAGD
                     SYLIAETVSRHLAKALGANMKVDAVGAAVALDAMQTSRPDGKTIMMFHDMTYLGVSFK
                     AYDDIYKLENMEIGPRVAQNPGAAWAAYLDAPYNTMAEIPEYLKANPEAKVRMACEAG
                     GVSHIGFIAFWEWVESKYGKDVADRIVVIVGGSTSDKIQLLIDRNADVIFADYTSLLD
                     YTKVGDKKIAMKFVGLLDNVEGIDVPSYADQGITLADGSEFRFSKDFLIYLPKNFPAE
                     MVKALDDGMKAVSEDPQFKADMAKMSYRGGYLDSAAAKKFIYQKRDSLQGLIDSAPSL
                     DDLVM"
     gene            2438..2893
                     /locus_tag="PK442_04455"
     CDS             2438..2893
                     /locus_tag="PK442_04455"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOO86929.1"
                     /translation="MFRVSYKPSTSHWLFPPIILGILALLLAVMIAQRAIECRRTGRP
                     FINREAMRFFEPGCDKLKLFGSVALFVLYIYAMEFAGFLASSIVVVFLYSVLFFGVDR
                     LKELPAAIASGRPWANGAFKSVCVSLFLSVLFSTSVWFLFARVFKITLP"
     gene            2910..>3637
                     /locus_tag="PK442_04460"
     CDS             2910..>3637
                     /locus_tag="PK442_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO86930.1"
                     /translation="MIDFGLLHLLMAFVGVVVGIVFGALPGMTATMAIAVFLPLTYAY
                     DLHVSLFLLLGLYVGGISGGLVPAILVNIPGTPSSICTGFDGYPMAKRGEAVRALRIG
                     ITASLFGGLFSLLLLFLFTPPLARVALSFSSVEKFLIILFALSIIAALSKGNMVTGVF
                     AGFLGVFFSLIGQFADNNKMRMVPDFLSVDLRNGFQLLPVLIGLFALVQILQEAEGGM
                     RDDAMGADLSREVRGFRFSDFKGQV"
BASE COUNT          615 a         1138 c         1164 g          720 t
ORIGIN      
        1 cagcatgctc ctcaacctca gcggagccca cggtccgtac ttcacgcgca acgtggtgct
       61 gctgacggac agcgagggga ggacgggcgt cggagaggtt ccggggggcg agaagatcgc
      121 cggggggctc gaacaggccc gcgacctcgt cgtcggctca cggacggggg agtacaagaa
      181 cacgctgctc gccgtgaaga agctcttcgc ggggcgggag gacgatgtgc ggggacagca
      241 gacattcgac ctgaggacag gggtgcatgt actcaccggc atcgaggcgc cgctgctcga
      301 tctgctgggg cagtatctcg gcgtgcccgc ggcgtcgctc ctcggcgacg ggcagctccg
      361 ggagaggatt cccgtcctgg ggtacctctt ctacgtggcg gacaggaaga agaccgatct
      421 cccgtacatc tcggagtccg gttcctccgt cgagtggtac aggctccgcc gcgaagaggc
      481 gacgacgccg gaggctatcg tcgcgcttgc aaaggcgagc cgcgatcttt acggtttttc
      541 ggatttcaag ctcaaggggg gcgtcttccc cggaagcgag gagatggagg ccgtgaggac
      601 gctgaagcgc gcgttccccg acgggcggat taccatcgac cccaacggag ggtggctgct
      661 gaaggaggcc gtcgaactgt gcagagggat gcacggtatc ctctcctact gcgaggaccc
      721 gtgcggggcc gagggaggct tttcggggcg cgaggtcatg gcggagttcc gccgcgccac
      781 ggggctgcct accgcgacga acatgatcgc caccgactgg cggcagatgg cccactcggc
      841 gatgcttcag tccgtggata tcccgctggc cgaccctcac ttctggacga tggagggttc
      901 ggtacgggtg gggcagttct gcgaggtttt cggcctcacg tggggctctc actcgaacaa
      961 ccacttcgat atctctctgg ccatgttcag ccacaccgcc gccgcggttc cgggcaggat
     1021 aacggccgtc gacacgcatt ggatatggca ggaggggacg gaacgcctca cgaaggagcc
     1081 tctcacaatc cgggacggat tcatcgacct ccccgcggct cccggtctgg gaatcgagcc
     1141 ggatatggag gcgatcgagg cggcgcaccg gctctacatg gagcactgcc tgggcgcgcg
     1201 ggacgacgcg gcggcgatgc agttccttat tccgggctgg aaattcgatc cgaagcgccc
     1261 ctgcatggtt cgctagggcg cttggagcac taaaaaccgg ggcctgcgct ccggaacaaa
     1321 aggaggagtt cacatgaagt tcagaaaggc tctgcttgct ctgctggtac tcgcgctgtt
     1381 ggccgttccc gccgtcgcgg gggcgaatcc gttcgccggg atgaacgtcc gcgtggtcat
     1441 cgggtccacc tcgaccgccg gcgattcgta cctgatcgcc gagactgtct cccggcacct
     1501 tgcgaaggcg ctgggcgcga acatgaaggt cgacgcggtc ggcgctgcgg tggcgctcga
     1561 cgcgatgcag acctcgcgtc ccgacgggaa gacgatcatg atgttccacg acatgaccta
     1621 cctcggcgtc tccttcaagg cctacgacga catctacaag ctggagaaca tggagatagg
     1681 cccccgggtc gcgcagaacc ccggcgccgc atgggcggcg tatctggacg ccccgtacaa
     1741 caccatggcg gagatccccg agtacctgaa ggcgaatccg gaagcgaagg tccgcatggc
     1801 gtgcgaagcg ggcggcgtct cccacatcgg tttcatcgcc ttctgggagt gggtcgagag
     1861 caagtacggc aaggacgtcg ccgacaggat cgtcgtgatc gtgggcggct ccacctccga
     1921 caagattcag cttctcatcg accggaacgc ggacgtgatc ttcgccgact acacatctct
     1981 ccttgactat accaaggtcg gggacaagaa gatcgccatg aagttcgtcg gccttctgga
     2041 caacgtggag ggcatcgacg ttccctcgta cgccgaccag gggatcacgc ttgccgacgg
     2101 gagcgagttc cgcttctcca aggatttcct catctacctt ccgaagaact tcccggccga
     2161 gatggtcaag gcgctcgacg atggcatgaa ggcggtctcc gaggatccgc agttcaaggc
     2221 ggacatggcg aagatgtcct accgcggcgg gtacctcgac tcggcggcgg cgaagaagtt
     2281 catctatcag aagagagatt ccctgcaggg gctgatcgat tccgccccgt ccctcgacga
     2341 cctggtgatg taacgcttcc cggacggagc ggcgcggaga acgcttttcg tttccccgcg
     2401 ccgctccctt tcgatactgc ctccggagga ttgagcgatg ttcagagtga gttacaaacc
     2461 ttccacgtcc cactggctgt ttccgcccat catcctgggc attctggcgc tgctcctcgc
     2521 ggtgatgatc gcgcagcggg cgatcgaatg ccgcaggaca gggcggcctt ttatcaacag
     2581 ggaagcaatg cgcttcttcg agcccggctg cgacaaactc aagctgttcg gctcggttgc
     2641 gctcttcgtc ctgtatatct acgcgatgga gttcgccggt tttctggcgt cgagcatcgt
     2701 cgtcgtcttt ctgtacagcg tactgttttt cggcgtcgac cgcctcaaag agcttccggc
     2761 ggccatcgcc tcggggaggc cgtgggcgaa cggcgcattc aaatccgtgt gcgtctcgct
     2821 gttcctctcc gttcttttct cgacctcggt ctggttcctc tttgcccggg tgttcaagat
     2881 aacgctgccg taaagaggag gaaacgcaca tgatagactt cggccttctt catctgctca
     2941 tggcgttcgt cggcgtggtc gtcggcatcg tcttcggcgc gctgccgggg atgacggcca
     3001 cgatggccat cgcggtcttt cttccgctca cgtacgccta cgacctgcac gtctccctct
     3061 tcctcctgct cgggctctac gtggggggaa tcagcggagg gcttgttccc gccatccttg
     3121 tgaacattcc gggaaccccg tcctccatct gcaccgggtt cgacggctac ccgatggcca
     3181 agcgcggcga ggcggtcagg gcgctgcgca tcggcatcac tgcctccctc ttcggcggcc
     3241 tcttcagcct gctgctgctc tttctcttca ccccgcccct cgccagggtc gccctcagct
     3301 tttcctcggt ggagaagttt ctgatcattc tcttcgcgct ctcgatcatc gccgccctct
     3361 ccaaggggaa catggtcacg ggcgtcttcg cggggttcct cggcgtcttc ttctccctga
     3421 tcggccagtt tgccgacaac aacaagatgc gcatggtgcc ggacttcctc tcggtggacc
     3481 tccgcaacgg cttccagctg cttcccgtgc tcatcggcct cttcgccctc gtacagatat
     3541 tgcaggaggc ggaggggggg atgcgggacg acgccatggg ggccgacctc tcccgcgagg
     3601 ttcgcggctt caggttctcg gactttaagg ggcaggt
//
LOCUS       DAOSWW010000159         3630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18063_length_3630_cov_3.381538, whole genome shotgun sequence.
ACCESSION   DAOSWW010000159 DAOSWW010000000
VERSION     DAOSWW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18063_length_3630_cov_3.381538"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PK442_04465"
     CDS             <1..1191
                     /locus_tag="PK442_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO86931.1"
                     /translation="RSLGFDSVVIDSYGNVVGEIIFSAEGPRLLLTAQMDHVDAGDVA
                     EWSKYPFGAFIEENRIYGRAASDQKGALASMMLAASLLKRDRSGVLQGRLFVAGAVHQ
                     ETFERVASKAIGDFADPDCVIVGEASGLLLERGQRGRAEIRVDIFGRMAHSSHPEFGV
                     NAADVMVEFLSFLKSRFTPPRDEFLGEGILVLTNLFSSPSYNRESSQREARPDSSAIP
                     ERCTAVFDRRLLLGETLEGVVRQFEEIVDEAVRVLPELRAKVSYPVMEDRCYTGAPIR
                     GSHYAPAWTIPSDAPFLRVLSAALEEAGLCPDVSARPGFGTNGCHYAAERLLPTVVYG
                     PSRRELVHTVDEYIEIDALVDSCRGYFAMAERLLASRTLCGQGKKRVSVKEEKRRKVH
                     GAAS"
     gene            1175..2422
                     /locus_tag="PK442_04470"
     CDS             1175..2422
                     /locus_tag="PK442_04470"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOO86932.1"
                     /translation="MAQPVELKCVLCGASYAPGEVEYTCPVCGLDGTLDVLYDYESAA
                     RTMTKGALAGRERSLWRYEEILPIVDKGSIPHLAVGWTPLYECPAVAEEYGVREFMVK
                     DDGRNPTGSLKDRASAVGVARAIDINQRTIACASTGNAASSLSGFAAVGGLRSFIFVP
                     EKAPVAKVTQLLVYGATVVLVKGDYADVFNLATAAINKYGWYNRNCAINPYLVEGKKT
                     CALEIAEQTGWDLPDRVFISVGDGCCIGGLYKGFSDLLRMGLISRMPKIVGVQAQGAK
                     PIYDAFKSKAERVAFGPADTVADSISVGAPRNWAKALRAVRESEGDMVAVSDEEILSA
                     IPELARKTGVFGEPAGAAAFAGFRKMAKEGLLGRGERIAVVVTGNGLKDIESARKSVG
                     APMLVEPTIEDFSGQLERSGFPC"
     gene            2416..>3630
                     /gene="glyA"
                     /locus_tag="PK442_04475"
     CDS             2416..>3630
                     /gene="glyA"
                     /locus_tag="PK442_04475"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOO86933.1"
                     /translation="MLEKGQEFLRDADPEIFAAIGEELARQRNGIELIASENFVSRAV
                     LSAMGSVMTNKYAEGYPHARYYGGCHVVDKAEDLARDRAKELFGCDHVNVQPHSGSQA
                     NMAVYFSVLQPGDTILAMNLAHGGHLTHGSPVNFSGKLYNIIPYGVDKTTERIDFAEV
                     ERLAVEHKPKMIVCGASAYPREIDAEHFRAIADKVGALLFFDIAHIAGLIAAGCHKDP
                     VPFCHFLTSTTHKTLRGPRGGMIMTTNEYAKAIDKNIFPGMQGGPLMHVIAAKAVAFR
                     EALKPSFKEYQKKVVANAKALAEEFLSLDYHLVSGGTDNHLILVNLMTKGVTGKAAEA
                     ALDKAGMTVNKNAVPFDTQSPFITSGIRVGTPAATTRGFGVDDMKQIARWIDRAVRNV
                     ENESELATIKAEV"
BASE COUNT          701 a         1027 c         1201 g          701 t
ORIGIN      
        1 cgttccctcg ggtttgactc cgtcgtgatc gattcatacg gcaacgtcgt gggagagatc
       61 atattctccg cggaggggcc gaggcttctc ctcacggcgc agatggatca tgtggatgcg
      121 ggagacgtcg ccgagtggtc caagtacccc ttcggcgcgt tcatcgagga gaatcgcatt
      181 tatggaagag ccgcctccga ccagaagggc gcgcttgcct cgatgatgct tgccgcgtct
      241 ctgctgaaac gagaccggag cggcgtgctg cagggcaggc tctttgtcgc cggagcggtg
      301 caccaggaga cgtttgaacg ggtcgcttcg aaggctattg gagattttgc cgacccggac
      361 tgcgtgatcg tcggcgaggc ctccggcctc ttgctcgaac gggggcagag ggggagggcg
      421 gaaatacgcg tcgatatctt cggcagaatg gcccactcgt cgcatcccga gttcggggtg
      481 aacgcggcgg acgttatggt cgagtttctt tcctttctca agagccgttt caccccgccg
      541 cgcgacgaat tcctcggcga gggaattctt gtcctcacca atcttttttc ctcaccgtca
      601 tacaacagag agagttctca acgggaggcc cgtcccgaca gcagcgcgat tcccgagcga
      661 tgcacagctg ttttcgacag gcggctgctg ctcggggaga cgctggaggg cgtcgtcagg
      721 cagttcgagg agatcgtgga cgaggctgtt cgtgttcttc cggagctccg ggcgaaggtc
      781 tcctaccctg tgatggagga tcgctgttat acgggggccc ccatcagggg gagccattac
      841 gcccctgcgt ggacgatccc ctcggacgcg ccttttttgc gcgtgctttc ggccgcgctg
      901 gaggaagcgg ggctctgtcc ggatgtgtcg gcgcgtcctg gcttcgggac caacggctgc
      961 cattacgcag ccgagcggtt gcttccgacc gtggtgtacg gaccctcccg gcgcgagttg
     1021 gtgcacacag tagatgagta tatagaaata gacgcgcttg tcgattcgtg cagagggtac
     1081 tttgcgatgg cggaacgcct cttggcgtcc cggactctct gcgggcaagg aaagaaacgc
     1141 gtttccgtga aggaagaaaa aaggaggaaa gtacatggcg cagccagttg agttgaaatg
     1201 tgttctgtgc ggagcttcgt atgctccggg agaggtggag tacacctgcc cggtctgcgg
     1261 actcgacggc acgctcgacg ttctgtacga ttatgaatcg gcggctcgta cgatgacgaa
     1321 gggggcgctt gcaggacgcg agcggtcgct gtggcgttac gaggaaattc tgccgatcgt
     1381 ggacaagggg agcatccccc atcttgcggt tggttggact ccgctgtacg aatgtcctgc
     1441 ggtcgccgag gagtacggcg tgagggagtt catggtgaag gacgacgggc ggaatccgac
     1501 agggtcgctc aaagaccgcg ccagcgcggt gggcgttgcc cgcgcgatcg acatcaacca
     1561 gaggacgatc gcctgcgcct ccaccggaaa cgccgcgagt tcactctcgg ggttcgcggc
     1621 ggtggggggg cttcgcagct tcatcttcgt tcctgagaag gcgcctgtcg ccaaggtgac
     1681 gcagcttctt gtctacggcg ccacggttgt cctggtgaag ggcgactatg cggacgtctt
     1741 caacctggcg acggcggcta tcaacaagta cggatggtac aaccggaact gcgcgatcaa
     1801 tccgtacctg gtggaaggga agaagacctg cgctctcgaa atagctgagc agacgggctg
     1861 ggatctcccg gacagggtgt tcatctctgt gggcgacggc tgctgcatcg gcggattgta
     1921 caaggggttc agcgatctgc ttcgcatggg actgatctcg cggatgccga agatcgtcgg
     1981 cgttcaggcg cagggcgcga agccgatcta cgatgcgttc aaaagcaagg cggagcgcgt
     2041 ggcattcggg cctgcggata cagtagcgga cagcatatct gtcggcgcgc cgcggaactg
     2101 ggccaaggcg cttcgggcgg tacgagagtc cgagggcgac atggtggcgg tctccgacga
     2161 ggagatcctg tcagcgattc ccgagcttgc ccggaagacc ggcgtttttg gggaaccggc
     2221 aggagccgcc gcgttcgccg ggttcaggaa gatggccaaa gaagggcttc tcggacgcgg
     2281 cgaacggatt gccgtagtcg tgaccgggaa cggtctgaag gatatagaga gcgcgcggaa
     2341 gtcggtgggg gcgccgatgc ttgtcgagcc gaccatcgaa gacttttccg gacaacttga
     2401 aaggagtggg tttccatgtt agagaagggg caggagtttc ttcgggacgc tgatcctgag
     2461 atttttgcgg caattggcga ggagctggcc cgccagcgga acggtatcga actgatcgcg
     2521 tcggagaact tcgtctcccg cgcggtgctt tccgccatgg gctcggtaat gacgaacaag
     2581 tacgccgaag ggtacccgca cgccaggtac tacggcggct gccacgtcgt cgacaaggct
     2641 gaggacttgg cgcgcgaccg ggcaaaagag ctcttcgggt gcgatcacgt caacgtacag
     2701 ccgcactccg gatcgcaggc gaatatggcg gtctacttct ccgtgctgca gccgggagat
     2761 actattcttg ccatgaacct cgcgcacggc ggccatctta cccacggatc gccggtgaac
     2821 ttctccggaa agctgtataa catcattccg tacggcgtcg acaagacgac cgagcggatc
     2881 gacttcgccg aggtcgagcg cctcgccgtc gagcacaaac ccaagatgat cgtctgcggc
     2941 gcttcggcct acccccgcga gatcgacgcg gagcatttcc gcgctatcgc ggacaaggtc
     3001 ggcgcgctgc ttttcttcga catcgcccat atcgccggac tgatcgccgc cggatgccac
     3061 aaggatccgg ttcccttctg ccacttcctt acctcgacga cgcacaagac gctccgcgga
     3121 cctcgcggcg gcatgatcat gaccacgaac gagtatgcga aagccatcga caagaacatc
     3181 ttccccggca tgcagggcgg tcctctgatg cacgtgatcg ccgccaaggc cgtggcgttc
     3241 cgcgaagcgc tcaagccgtc cttcaaggag tatcagaaga aggttgtggc gaacgcgaaa
     3301 gcgctcgcgg aagagttcct ctccctcgat tatcatctgg tctccggcgg tacggataac
     3361 cacctgattc tggtgaacct gatgacaaag ggcgtcaccg gaaaggctgc ggaagccgcg
     3421 ctggataagg ctggaatgac ggtaaacaag aatgctgtgc cttttgatac ccagagcccc
     3481 ttcatcacca gcggcatccg ggtcggcacg cccgcggcca cgactcgcgg cttcggcgtc
     3541 gacgacatga agcagatcgc ccgctggatc gaccgcgctg tgcggaacgt cgaaaatgaa
     3601 tccgagcttg cgacgatcaa ggccgaggtt
//
LOCUS       DAOSWW010000160         3601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18274_length_3601_cov_3.915962, whole genome shotgun sequence.
ACCESSION   DAOSWW010000160 DAOSWW010000000
VERSION     DAOSWW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18274_length_3601_cov_3.915962"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..729
                     /locus_tag="PK442_04480"
     CDS             73..729
                     /locus_tag="PK442_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO86934.1"
                     /translation="MMTYWNRVFYFFVKWFFFIALKIYNRLSIRWTSSLPQRNVIVVA
                     NHASNLDPVIMGAVFPRRLRYLAKEELFKPFIFGNMVRALGSIPVRKSDSQSAGTALR
                     TFLKLLESGENVLIFPEGSRSLNGELQPLEGGVALIALKSGAPIIPSFISGTFEAMPP
                     GSRWVKPLHLSIVFGDTIDSAKYIPFGKEGREMLLKDLNEVFISLSTADMPGKSRNNS
                     "
     gene            726..1829
                     /gene="hflX"
                     /locus_tag="PK442_04485"
     CDS             726..1829
                     /gene="hflX"
                     /locus_tag="PK442_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOO86935.1"
                     /translation="MRKAILAAIELPKQEFAPTVLLDELTLLLENLGIQTAGTVIQRR
                     ERPDSAFLLGKGKVDEIALFCKAAGADLVVCNESLTPGQKSNLQHTIGLEVWDRPFVI
                     MKIFEKRARTSEAKLQVEMALCRYEIPHLKGLGAQMSRLGGGIGTRGPGETEFERHRR
                     KLERRVRDIGRKLEAMKHKRALQRDRRRKMDVPVVSLVGYTNSGKSSILRALSGDTSL
                     IAENRLFSTLDTFMRRVDLPSGRTVLLSDTVGFIRDLPPGLVTAFRTTLEEIVASTFL
                     IVVLDISTTDVQEIRDVVEHTLHEIGAGEIPRLFVLNKIDLTSPEYLDATTARLGIEH
                     SQSIATSVLEKRGLHELLDSMDLFLEKMEVSQI"
     gene            1826..2710
                     /locus_tag="PK442_04490"
     CDS             1826..2710
                     /locus_tag="PK442_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOO86936.1"
                     /translation="MIRSMTGFTRETRAFEWGTLTVELSSVNHRYQEISPRLPREMAS
                     LEPLITGRLRQGVGRGKIRCSVELHWAPQFRTATLDATVLSNYHTQLAELAERLGTGE
                     TLPLASLLSLPGVFESPSVLAGIEDELNAALESLLEQAIEHLQAMRSTEGTNLLHAIE
                     EYLNAFESLLNSLEKAWDPKRDQAIEDMRSRITNLLEAMSIEVDQNRVAQEVVILSDK
                     WDIAEEFVRSRSHLKQFAAVLNGKSSEGRKLDFLLQEMNREINTMGSKISDAELRWMV
                     VEAKSLLEKIREQVQNVE"
     gene            2718..2987
                     /locus_tag="PK442_04495"
     CDS             2718..2987
                     /locus_tag="PK442_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOO86937.1"
                     /translation="MTGRLVHIGFGNMLVAERIVAIVHPSSAPIKRLKEEAREDGRLI
                     DATQGRKTRAVLITDSNHVVLSAIQPETIVNRYEEEAEDEKTNDG"
     gene            2965..3552
                     /gene="gmk"
                     /locus_tag="PK442_04500"
     CDS             2965..3552
                     /gene="gmk"
                     /locus_tag="PK442_04500"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOO86938.1"
                     /translation="MKKRTTGSLFVLSGPSGAGKGTLRKKMFEKVPGITFSISCTTRE
                     PREGEREGIDYHFISADEFQSRLERGEFLENADVHGHLYGTLREDAVRVLNSGSDILL
                     EIDVQGALQVREKMPGSILLFVAPPSFEELEQRLRDRGTESEERLQIRLRNARWEMEK
                     APLYDYVIINDDIERAAEELEGIILEQRKNREARR"
BASE COUNT          940 a          847 c          950 g          864 t
ORIGIN      
        1 acgctctttc tatcgaagaa gccgtagcga cgctcgcttc gatcatctca aaacgaatag
       61 gagcgtgacc ggatgatgac gtattggaac cgcgttttct atttttttgt caaatggttt
      121 ttcttcattg ccttgaagat ctacaaccgt ttgtccatca gatggacgtc ttcactccct
      181 caacgcaacg tcattgttgt cgccaatcat gcgagcaatc tggatcccgt aatcatgggc
      241 gctgtattcc cgcggcgtct tcgctatctt gcgaaggaag agctcttcaa accgttcata
      301 ttcggcaata tggtcagggc gcttggctcc atacccgtac gaaagagcga cagccagagc
      361 gcagggacag ccctccgcac tttcctgaaa ctcctggaaa gcggggagaa cgtattgatc
      421 tttccggagg gaagccgttc tctaaacggg gaacttcagc ctctcgaagg aggggtggcg
      481 ctcattgcgt taaaatccgg agcgccgatc attccctctt tcatctccgg tactttcgag
      541 gcaatgcccc ccggaagccg ctgggtaaaa cctcttcatc tttcgattgt ctttggggat
      601 accattgatt cggcgaaata catccctttc gggaaagaag gaagagagat gttgctcaag
      661 gatcttaacg aggttttcat ctctttgagc actgctgata tgcctggcaa atccaggaac
      721 aattcatgag aaaagcaatc cttgcggcga ttgaacttcc aaagcaggag tttgctccga
      781 cagtcctcct cgacgaactg acgcttcttc ttgaaaatct tggaatccaa acggcaggga
      841 ctgtaataca gcgccgcgaa cgaccggatt cagcgtttct tcttggcaag gggaaagtcg
      901 acgaaatcgc tcttttttgt aaagctgcgg gggctgacct cgttgtttgt aacgaaagcc
      961 tcaccccggg acagaagagt aatctgcagc atacaattgg cctggaagtc tgggaccgtc
     1021 cttttgtcat catgaagata ttcgaaaagc gcgcgaggac gtccgaagca aagctgcagg
     1081 tcgagatggc gttgtgcaga tacgaaattc ctcatctcaa agggttggga gcgcagatgt
     1141 cgcgccttgg aggaggtatc ggaacacgcg gtccgggtga aaccgaattt gaaagacatc
     1201 gtcggaaact tgaacgaaga gtccgggata tcggcagaaa acttgaggcg atgaaacaca
     1261 agagggcgct ccaaagagat cgccgcagga agatggatgt accggttgtt tctttggtcg
     1321 gttatacaaa cagcggaaaa tcttcgatcc tgagagcatt aagcggcgac acctcgctta
     1381 tcgcggaaaa caggcttttc tcaacactgg atacgttcat gcgcagggtt gatctcccct
     1441 ccgggaggac agttcttctg tccgatacgg tcgggtttat acgagatctt cctcccggtc
     1501 tggtaacagc gttccgtacg actctcgaag agatagtcgc atctacattt ctcatagttg
     1561 ttctcgatat ctctacaacc gacgttcaag aaatccggga cgtggtagag cataccttgc
     1621 acgaaatagg cgcaggggaa attcctcgtc ttttcgtctt gaacaagatc gacctgacct
     1681 caccagaata tttggacgcg acaacggctc gcttaggcat tgaacactcg cagtcaatcg
     1741 cgacaagcgt ccttgaaaag cgaggattac atgaactact cgattcaatg gatctttttc
     1801 ttgaaaaaat ggaggtctcc caaatatgat acgcagcatg accggattta cgcgggaaac
     1861 ccgcgctttt gaatggggaa cgctcaccgt ggaactctcc tcggtaaatc atagatatca
     1921 ggaaatttca cctcgtctgc ctcgggagat ggcgtcattg gagcctttga tcacaggaag
     1981 actgagacaa ggcgttggca gaggtaaaat tcgttgttct gtggaattgc actgggctcc
     2041 gcagtttcga accgccacac ttgacgcaac tgtgctttcg aattatcata cacagcttgc
     2101 ggagttggcc gaacgtctcg gaactggaga aactcttcct cttgcgtcgc ttttatcgct
     2161 gcccggcgtt tttgaatctc cttccgttct cgcaggcatc gaagatgaac tgaacgcagc
     2221 gcttgagtct ctgttggaac aagccataga acatcttcaa gcgatgcgaa gtacggaggg
     2281 aacaaatctc ttgcacgcca ttgaagagta cctcaacgcg tttgaatccc tcctcaattc
     2341 tttggagaaa gcatgggacc ccaagcgaga tcaggctatc gaggatatgc gatcaagaat
     2401 tacgaacctt ctggaagcca tgtcgataga ggttgaccag aaccgggttg ctcaggaagt
     2461 tgttattctc tcagacaagt gggatattgc cgaggagttt gttcgttcac gaagccacct
     2521 gaaacagttt gctgccgtgc tcaacggaaa atcatcggaa gggcgcaaac tcgattttct
     2581 tcttcaagag atgaaccggg aaattaacac catggggtcg aagatttccg acgctgaact
     2641 ccggtggatg gtcgttgaag cgaagtcgct ccttgaaaag atcagagaac aagtacaaaa
     2701 cgtggagtga taagaatatg accggaaggc tcgttcacat tggttttggc aatatgcttg
     2761 tcgctgaacg tatcgtcgct attgtgcatc cgtcctccgc tcctataaag cgcctgaagg
     2821 aagaggcaag agaggacggt cgcctgattg acgcgacgca aggccgaaag acacgggcag
     2881 ttttgattac agacagcaat catgtcgtcc tctctgcgat tcagccagag acgatagtca
     2941 atcgatacga agaagaggct gaagatgaaa aaacgaacga cgggtagtct ttttgtcctc
     3001 tcaggtccta gcggggcagg gaaaggaaca ctccggaaaa aaatgttcga gaaggtgccc
     3061 ggtatcacct tctcgatttc gtgcactacc cgcgagccgc gcgaaggtga gcgcgaagga
     3121 atcgactatc attttatttc tgcggatgag ttccagtcac gtcttgagag aggcgaattt
     3181 cttgaaaatg cggatgtcca cggccactta tacggtactt tgcgcgaaga tgcggtgcgt
     3241 gtgctgaatt caggaagcga catcctcttg gaaatcgacg ttcagggggc tttgcaggtc
     3301 agggagaaga tgcccggaag tatcctgctg ttcgtcgcgc caccctcgtt tgaagagctt
     3361 gaacagcgtc ttcgtgacag aggaacggaa tcagaggaac gtttacaaat tcgccttcga
     3421 aatgctcgat gggaaatgga aaaggcgccg ttatatgatt atgtgataat taacgatgat
     3481 attgagaggg ccgcagaaga actcgaaggt atcatccttg aacagagaaa gaacagggag
     3541 gcgcgaagat gaaattttat gatttggatt ctctcgcaga aaagcagggt ctggataaca
     3601 a
//
LOCUS       DAOSWW010000161         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18300_length_3598_cov_3.804403, whole genome shotgun sequence.
ACCESSION   DAOSWW010000161 DAOSWW010000000
VERSION     DAOSWW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18300_length_3598_cov_3.804403"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>354)
                     /locus_tag="PK442_04505"
     CDS             complement(<1..>354)
                     /locus_tag="PK442_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO86939.1"
                     /translation="GYAINQDVRHLTGAVYDEQCLRDLGKLAVEKDITILYDEIYERL
                     VYGNAVHHQILNLVPEAREHTIIINGVSKAFAMTGWRIGYALGPSSIMGYLGDLQGHV
                     TSNACSVAQWASVGAL"
     assembly_gap    355..364
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<365..901)
                     /locus_tag="PK442_04510"
     CDS             complement(<365..901)
                     /locus_tag="PK442_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO86940.1"
                     /translation="MYVSTRARKMEPSATLAVTAKAKSLKREGKPVISFGAGEPDFNS
                     PPSALRFAEEAMKKGQTHYTPGTGIAELREAVCAYYKEHFGLEYKPADVVVGAGAKPL
                     LYEALGCLLDPGDEVLVFTPAWVSYVEQIRFFDGKAVLIDTSKTDFVPRIEDVGKATT
                     ARTRAMIINTPNNPTGAVY"
     gene            1044..1580
                     /gene="raiA"
                     /locus_tag="PK442_04515"
     CDS             1044..1580
                     /gene="raiA"
                     /locus_tag="PK442_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOO86941.1"
                     /translation="MDIRFVTRGVEMKDELKDYMENKMSKLERFFNRIIDSQIVVSFG
                     RGMYTVEITSNANGVIMRGQENDSDMRKAFDQALKNLERQIKRHKSYLKDRAQLKTHD
                     ISFEIPVVEPELPIPPEEEIVKVKRFPLRPMSAKEATMQMDLLGHEFFLFKNAESGIV
                     NVVYKRKDGGYGLLEPSE"
     gene            1770..2111
                     /gene="hypA"
                     /locus_tag="PK442_04520"
     CDS             1770..2111
                     /gene="hypA"
                     /locus_tag="PK442_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOO86942.1"
                     /translation="MHELSLADAIVSALLKMCEDEAWGRVKKVRLRVGALRQVFPEML
                     EFAFSTVVKDTALSGALLEIEEIPLEWRCLECGNRWREDSGVCPLCGSFNRETLAGME
                     LEIHSVEVEET"
     gene            2108..2773
                     /gene="hypB"
                     /locus_tag="PK442_04525"
     CDS             2108..2773
                     /gene="hypB"
                     /locus_tag="PK442_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOO86943.1"
                     /translation="MNKIVTLQQSVMAEDEKFAAAIRDRNTRMGLLMVNIIGSPGCGK
                     TTLLEKTALHAGLSMAVIEGDLATDRDAERLARLGVPVVQINTKGGCHLEANVVEKAM
                     ESLPLDTLDVLFIENVGNLVCPAEFDLGEDFKVAVLSAPEGADKPLKYPYLFTSAKAV
                     LLTKTDLLPHLSFDMEMFAEDTRKLNPAAPVFHVDSLSGAGIDQWVGFIKLKAKEKKA
                     NVR"
     gene            2763..>3598
                     /locus_tag="PK442_04530"
     CDS             2763..>3598
                     /locus_tag="PK442_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO86944.1"
                     /translation="MFAEQRPNKGLKMAEAERDLLEKLNPRQREAVAYCDGPLLVLAG
                     AGSGKTRVLTHKIAYLIAAGAAKPWDFLAVTFTNKAANEMKDRVRSLVGEGGGDMQVC
                     TFHSFGLRFLFRNRSHLPSVGLREGFAIFDRGDSRSLVKEIIESFNLDPKQFEAAALL
                     DRISTVKNDCRSPSSGQSPLLEGVYGDIFTAYNEALRRQNAVDFDDLLLLPLQLLSGD
                     EELRSREQSRLSWILVDEYQDVNRPQYLLLRKLVGDRNKIMVVGDPDQSIYGWRGADM
                     GM"
BASE COUNT          843 a          926 c          984 g          835 t
ORIGIN      
        1 caacgcaccg acggaggccc actgggcaac agaacacgcg ttcgatgtga cgtgtccctg
       61 aaggtcgccc aaatacccca tgatactcga agggccaagg gcgtatccga tacgccagcc
      121 ggtcatcgca aaagccttgc tcacgccgtt gatgatgatg gtatgctctc gcgcctccgg
      181 aaccaggttc aaaatctggt ggtgcacagc gttcccgtag acaaggcgtt cgtagatctc
      241 gtcgtaaagg atagtaatat ccttctcgac agcgagtttg ccgaggtctc gaagacactg
      301 ttcgtcatac accgcgccgg tcagatgacg cacatcctgg ttgatggcgt aaccnnnnnn
      361 nnnnatacac agcgccggtg gggttgttcg gcgtattgat aatcatggcg cgcgttctcg
      421 ccgtcgttgc ttttccgaca tcctctatcc gggggacaaa atcggttttg ctcgtgtcga
      481 tgagcaccgc tttcccgtcg aaaaagcgaa tctgctccac gtaactcacc catgcaggcg
      541 taaaaacaag cacttcatca cccgggtcga gaagacagcc gagcgcctcg taaagcaaag
      601 gcttggcccc ggcgcccaca acaacgtcgg ccggtttgta ctcaagcccg aaatgctctt
      661 tgtaataggc gcatacagct tctctcagct cggcgattcc tgttcccgga gtgtagtgcg
      721 tttgcccctt tttcatcgcc tcttccgcga agcgaagagc agagggaggc gaattgaaat
      781 caggctctcc tgcgccgaag gagataacgg gtttgccctc ccgcttgaga ctttttgcct
      841 ttgccgttac agcaagggtc gccgaaggtt ccatcttccg ggccctagtc gaaacataca
      901 tttcacagta cctcctgaat gatatttttt tgtgtgatgt atactcattc tttaaaaaac
      961 cccgagtgaa agcctttgtt gcccttctcc ttacccggtg ctagaataat accatattac
     1021 agagtctgaa tggagggatt tgcatggaca tacgtttcgt gacccgtggc gtcgaaatga
     1081 aagatgaact caaggattac atggagaaca aaatgtccaa gcttgagagg tttttcaacc
     1141 gcatcatcga cagccagatc gtggtgagtt tcggacgtgg tatgtacact gtcgaaatta
     1201 cctccaacgc caacggggta ataatgagag ggcaagagaa cgattccgat atgaggaaag
     1261 ccttcgacca ggcgctcaaa aaccttgaac gccagatcaa acgccacaag agttatttga
     1321 aggacagagc gcagctgaaa acgcacgaca tctcctttga gattcctgtc gtggagcctg
     1381 agcttcctat ccctcccgaa gaggaaatcg tgaaggtgaa acgtttcccc ctgcgtccta
     1441 tgagcgcaaa agaagcgacg atgcagatgg atcttcttgg gcatgagttt ttcttgttca
     1501 agaacgccga gtccgggata gtgaacgtgg tgtacaagcg gaaggacggc ggatacggcc
     1561 ttcttgagcc ttcggagtga ttttcagtcg cagcgggcgc ccctcgcgcg ggggcttcgg
     1621 atcgcggtaa cttgatctgt accggtgaat gtgtgtattc tccggttttt tcccccgcct
     1681 gaacgggtat ggcaggtttt tccccgtccg taaccgtcga tgcgaaaagg acccgaaagg
     1741 gtcctttttt tctggaaaag aggaacggca tgcatgaact ctctcttgcc gatgcgattg
     1801 tatcggccct tttaaaaatg tgcgaggacg aagcatgggg cagggtaaaa aaagttcgcc
     1861 ttcgggtcgg cgcccttcgc caggtttttc ccgaaatgct cgaattcgca ttcagtacag
     1921 tggttaaaga tacggcgctt tccggggcgc tcctcgaaat cgaggagatt cctctggaat
     1981 ggagatgcct tgaatgcggg aatcgctggc gggaggattc cggagtctgt ccgttatgcg
     2041 gctctttcaa cagggagacg cttgcaggga tggaactgga aattcattct gtggaggtcg
     2101 aggaaacatg aataagatag ttacgctgca acagtccgtg atggcggagg atgagaaatt
     2161 cgccgccgcg atcagggaca gaaatacccg aatgggcctt ctgatggtca atatcatcgg
     2221 ttctcccggt tgcgggaaga caaccctgct ggaaaaaaca gcgcttcatg caggactctc
     2281 gatggctgta atcgaaggag atcttgccac agaccgcgat gcggaacgat tggctcgcct
     2341 tggcgtaccc gtcgtgcaga tcaacacaaa aggcggatgc caccttgaag ccaacgttgt
     2401 tgaaaaagcg atggaatcgc ttccgctgga tacgctggac gttcttttca tcgagaacgt
     2461 cggcaacctt gtctgcccgg cggaattcga cctcggtgag gatttcaagg ttgcggtgct
     2521 gagcgctccg gaaggtgccg ataaaccgct gaaatacccg tatctcttca cttccgcgaa
     2581 agcggtgctg ctgaccaaaa ccgatctgct tccccacctc tctttcgaca tggagatgtt
     2641 tgcggaagat acgcgaaaac tcaatcctgc cgcgcccgtg tttcacgtcg attctctttc
     2701 gggcgccggt atcgatcagt gggtcggctt tataaagctc aaagcgaagg agaaaaaagc
     2761 gaatgttcgc tgaacagcgc ccaaacaaag gattgaagat ggcagaagct gaacgggatc
     2821 tgttggaaaa gctgaatccc cgccagcggg aggctgtggc gtactgtgac ggccctcttc
     2881 ttgtgctcgc cggcgccgga agcggaaaaa cgcgcgtcct cacgcacaag atagcctacc
     2941 ttattgccgc cggagccgcc aaaccatggg actttcttgc cgttaccttc acgaacaaag
     3001 cagcaaacga gatgaaagat cgcgtccggt ctcttgttgg agaagggggc ggcgatatgc
     3061 aggtgtgcac attccactct ttcggattgc gtttcctttt ccgaaacagg tcccatctgc
     3121 cttccgtcgg gcttcgggaa ggattcgcta tcttcgaccg gggagacagc cgctccttgg
     3181 tgaaggagat tatagaaagt tttaaccttg atccgaagca gttcgaggct gccgcgctgc
     3241 ttgataggat ttccaccgta aaaaacgact gccgttcccc ttcgtcgggg cagtctcctc
     3301 ttctagaggg agtctacggc gatatcttca ccgcgtacaa cgaagcgctc cggcgtcaga
     3361 atgcggtcga cttcgacgat cttcttttgc tccctttgca acttctttcg ggagacgagg
     3421 agctgcgttc acgagaacag tcgagacttt cctggatatt ggtggatgaa tatcaggatg
     3481 taaaccggcc tcaatatctt cttttacgaa agcttgtcgg cgaccgaaac aaaataatgg
     3541 tggtcggaga tcctgatcag tcgatctacg ggtggcgcgg cgccgatatg ggaatgat
//
LOCUS       DAOSWW010000162         3594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18332_length_3594_cov_3.594236, whole genome shotgun sequence.
ACCESSION   DAOSWW010000162 DAOSWW010000000
VERSION     DAOSWW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18332_length_3594_cov_3.594236"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1456)
                     /locus_tag="PK442_04535"
     CDS             complement(173..1456)
                     /locus_tag="PK442_04535"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO86945.1"
                     /translation="MAATIFAVFTLLLVIRMPVSFSLAISAAVALVFAGSMDPLIVVQ
                     RMYTSVESFSLIAIPFFILSGGFMEHGGISRRLISFSSSLVGHIRGGLSMISIVAAMF
                     FAGISGSTAADTAAVGSVLIPAMEKKGYGKDLATSVVATAGAIGIIIPPSIPMIILGI
                     TGSISIGGLFLGGVFPGILMGLALMVTSSLFAKKRNLPSEVRATWTERRHAFTDSILA
                     LMTMVIIMGGILSGVFTATEASVVAAFYSFIVGRFVYGELKWSAIPEILVKTGITTGV
                     VVLCIATASSFGWILAAEQIPDKIAKAVFALSENRLVILFLMNALMLFMGTFLDISPI
                     IIILVPILFPIARQLGVTPIHFGIMTIVNMAIGQCTPPVGISLFVATGIAKAKLGEIL
                     GAYMKYLLTMIVVLAIITIFPQIITFLPQMVMGLK"
     gene            complement(1458..1940)
                     /locus_tag="PK442_04540"
     CDS             complement(1458..1940)
                     /locus_tag="PK442_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO86946.1"
                     /translation="MERVDALVGRMERWMLILLLTAMVALGLLQIISRFVIKAPITWS
                     EALLMYMFVWFSFIGASLAVKDLAHFEVEIFMVRLPQPLQKTVAVVVYCLIFAFALFM
                     VWKGVFLVKLNQRQLMAMMPFTMSVPYVILPLSGACMAIHSLKHVVGLIGRHSSKWSD
                     "
     gene            complement(1946..2812)
                     /locus_tag="PK442_04545"
     CDS             complement(1946..2812)
                     /locus_tag="PK442_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOO86947.1"
                     /translation="MEKKNGVLLQAPAETKESGVSVPVFSGDDAESVQRESGASIRVL
                     DRAVAVLGFMAEQKRQVGITEIAEATHLSKATVHRILSTLRDYSVVLKDESGRYQMGP
                     SVLYWADAYRKHSGLAELARPYLHTLWEIARETVHLFVFEAGEAYYIDKLDSPHPVGM
                     RSRIGARRDLYSTSGGRAILSFLPEAELKSYLDRTELRPRTRRTITDRKVLEEFLAEC
                     RKRGFCEEVEENEEGIRCVGAAVLDLRGYPVGAVSVSAPAYRFDDRASLATGERIRET
                     AMAISRELGYRG"
     gene            3090..>3594
                     /locus_tag="PK442_04550"
     CDS             3090..>3594
                     /locus_tag="PK442_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86948.1"
                     /translation="MSRSIVTRLALFMIMGMLVLGGALAFLARMGVDELSRAMESTTD
                     RILDEDIRNKVLNDEQTAEAYGQAMAGYLAWIALPPLWDYNYKNLSDYAAGMLNVPNV
                     LAAVIYKEDGSIAAGEMMIGSKNGGKDSRVFTSDIVREGQKIGSVELVLSTAHLEQLR
                     QKSEETRE"
BASE COUNT          803 a         1097 c         1028 g          666 t
ORIGIN      
        1 tttttcgaca taatgctacc tccttcgggt atcagctgat ggactgctgt tctttccctc
       61 gtcgccgccg aggggtgaat cctgttcgcg ctgttcttag caaactccgc cgcctgttcc
      121 caccgccctt cgttccatgt ccggccgggc gagaagcccc gccgtcgcag aattacttca
      181 atcccatcac catctgcggg aggaacgtaa tgatctgcgg gaagatggtg atgatcgcaa
      241 gcacgacgat catggtaagc aggtacttca tgtacgcccc caggatctcc cccagcttcg
      301 ccttcgcgat gcccgtcgcc acgaagagag agatgcccac aggaggcgta cactgaccga
      361 tcgccatgtt cacgatggtc atgatgccga agtgaatcgg cgtcacgccg agctgacggg
      421 ctatcgggaa gaggatgggg acgaggatga tgatgatcgg ggagatgtcg aggaaggtcc
      481 ccatgaagag catcagcgcg ttcatcagga agaggatgac cagccggttc tccgagaggg
      541 cgaagaccgc cttcgcgatc ttgtcgggga tctgctccgc ggcaagaatc cacccgaagc
      601 tcgacgccgt ggcgatgcac agcaccacca cgccggtggt gatccccgtc ttcaccagaa
      661 tctccggaat cgcgctccac ttcagctcgc cgtagacgaa ccgccctacg ataaacgagt
      721 agaacgccgc caccacggac gcctccgtcg cggtgaagac ccccgagaga atgcctccca
      781 tgatgatgac catcgtcatc agggcaagaa tggagtcggt gaacgcatgc cggcgttccg
      841 tccatgtagc gcgtacttcg gaaggaaggt tgcgcttttt ggcgaagagc gaactggtga
      901 ccatcagggc gagccccatc aggatgccgg ggaagacgcc gccgaggaag agcccgccga
      961 tggagatgct ccccgtgatg ccgaggatga tcatcgggat gctcggcggg atgatgatgc
     1021 cgatagctcc cgccgtcgcc accacgctgg tcgccagatc cttgccgtac ccttttttct
     1081 ccatcgcggg gatgagcacg ctgcccacgg cggcggtgtc tgccgccgtg gagccggaga
     1141 ttcctgcgaa gaacatggcc gcgacgatgc tgatcatgga gagcccgccc ctgatatgcc
     1201 cgacgaggct cgacgagaag gagatcaggc ggcgcgagat cccgccatgc tccataaatc
     1261 ctcccgagag aatgaagaac gggatggcga tcagggagaa cgactccacc gaagtgtaca
     1321 ttctctgaac gacgatgagc gggtccatgc tccccgcaaa gacgagggca acggccgccg
     1381 aaatggcaag cgaaaaggag accggcatac ggatgacgag cagcagcgtg aaaaccgcga
     1441 aaatagtggc agccatgtta atcactccac ttcgaagaat gacggccgat aaggccgacg
     1501 acgtgtttca gcgaatgaat cgccatgcag gcgccgctca gcggcagtat cacgtagggg
     1561 acgctcatcg taaagggcat catcgccatg agctgcctct ggttgagctt caccagaaaa
     1621 acccccttcc agaccatgaa gagggcaaag gcgaagatca aacagtacac gacaacggca
     1681 accgtcttct gcagcggctg cggaagacgg accatgaaaa tctcgacctc gaagtgcgcc
     1741 agatccttca cggcgagact tgcgccgatg aagctgaacc agacgaacat gtacatgagg
     1801 agggcctccg accacgtgat cggcgctttg atgacgaacc ggctgatgat ctggagaagc
     1861 cccagcgcca ccatcgccgt cagaagaagg atcaacatcc agcgttccat gcgtcctaca
     1921 agagcgtcta cacgttccat gagttttacc ccctataccc cagctcgcgg gagattgcca
     1981 ttgccgtctc ccgtattcgt tcccctgtcg ccagagaggc tctgtcgtcg aaacggtagg
     2041 ccggagcgct cacgctcacc gcccccacgg ggtagccccg cagatcaagc acggcggccc
     2101 ctacgcagcg aatcccctcc tcgttctcct ccacctcctc gcagaagccg cgtttgcggc
     2161 attcagcgag aaactcttcc agcaccttcc tgtccgtgat ggttctcctg gttcggggac
     2221 ggagctccgt cctgtcgagg taggatttca gctccgcctc gggaaggaag gaaaggattg
     2281 cgcggccgcc ggacgtgctg tagagatccc gccgcgcgcc gatgcgggaa cgcatgccca
     2341 cagggtgcgg gctgtcgagc ttgtcgatgt agtacgcctc ccccgcctcg aagacgaaga
     2401 gatgcaccgt ttcccgggcg atctcccaca gcgtgtgcag atacggacgc gccagctcgg
     2461 ccagccccga gtgtttccgg tacgcatccg cccagtagag gacggagggc cccatctgat
     2521 accttcccga ctcgtccttc agaacgacgg agtagtctcg gagcgtcgag agaatacggt
     2581 gcaccgtcgc ttttgaaaga tgcgtcgcct ccgctatttc cgtgatacct acctgccgtt
     2641 tctgctctgc catgaagccg agcacggcaa ccgcacggtc aagcaccctg atggaggctc
     2701 ctgactcccg ctgcacgctc tcggcgtcgt ctcccgaaaa aacaggaacg ctgacgccgc
     2761 tctcctttgt ctccgcagga gcttgtaaca gtacgccgtt cttcttctcc ataataattc
     2821 cacctgccaa aatagcgatt ctttggatga aacaacacta aggtacgata caggggcgag
     2881 gatgtcaact ctttttcagt ggaacttttc cgttgaatcc aagaagtcga acaaaaagaa
     2941 aaaactttca ctgatcgctt ccctgctgta caatgtctga aaccagcgct gcatctcccc
     3001 tgcgcgaaga tccggcgttt ggtatcgtat tccgaaagac aagggcaact cccgcctttt
     3061 tgcgcgcaac cggacaggag gagtgctgta tgtccagaag tatcgtcaca cgactcgccc
     3121 tttttatgat catgggcatg cttgttcttg gaggagctct ggcgtttctc gcccgaatgg
     3181 gcgtggacga actctcccgc gctatggagt ccactaccga ccgcattctg gacgaggaca
     3241 tacggaataa agtgctgaac gacgagcaga ccgccgaagc ctacggacag gccatggcgg
     3301 ggtacctcgc ctggatcgcg ctccctcccc tgtgggacta caactacaag aacttgtccg
     3361 actacgccgc aggaatgctc aacgtcccca acgtcctcgc cgcggtcatc tacaaggaag
     3421 acggttcgat cgcggcgggc gagatgatga tcggttcgaa gaacggcggg aaagactccc
     3481 gggtgttcac atccgatatc gtgcgcgagg ggcagaagat cggctccgtg gagctcgtcc
     3541 tcagcaccgc acaccttgag caactgcggc agaagagcga agaaacgcgc gaag
//
LOCUS       DAOSWW010000163         3594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18334_length_3594_cov_2.823962, whole genome shotgun sequence.
ACCESSION   DAOSWW010000163 DAOSWW010000000
VERSION     DAOSWW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18334_length_3594_cov_2.823962"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..966)
                     /locus_tag="PK442_04555"
     CDS             complement(67..966)
                     /locus_tag="PK442_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HOO86949.1"
                     /translation="MLSVSDVVFSFSPEHPPVARVSPGDMVRFMTKDCFCDQVTGASD
                     LVTAIDFDRVNPATGPVFVEGAEPGDTLCAEILSIDVAEKGIACTLPDSGPLIGWCEL
                     RTKVIEVRDGKAFFNDLVFPVRPMVGVIGTAPAEGAVVCGQPGAHGGNLDCKFMTAGA
                     KAYFPVRTRGALFQLGDLHASMGDGEIGGTGIEIGGTVLVRLHLIKNLPLDWPMLETG
                     DMIHTLASGPEFVAVQREAARQMQRLIAAAWGWDETDAALYLSVRGDSAICQTCQPST
                     FDMVLRMGIPKEPGKARLLPMPG"
     gene            complement(1087..1671)
                     /locus_tag="PK442_04560"
     CDS             complement(1087..1671)
                     /locus_tag="PK442_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOO86950.1"
                     /translation="MRNGIWGAALCAILSAAIFLAGVFAVKLYAQEIPGTSVGRKAAI
                     GIMSREISAAMTPWKIKGPIRKATEYSRHIWDACELYKVDPFLVAALVCVESEGRADA
                     VSHSRVGLMQVNVQENMAWITKHFPKIKTVDDLLKARNNVMAGAFALSAAMRSSEDVR
                     TALFKYLGTEDERQVGKILDVVGRMNTAAQAGRK"
     gene            1842..2084
                     /locus_tag="PK442_04565"
     CDS             1842..2084
                     /locus_tag="PK442_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOO86951.1"
                     /translation="MSDITATEARKRLFQLLDEVADTHVPVRIAGKRNAAVLVSESDW
                     RAMEETLFLCSIPGMRDSIIEGMATPVEECTEDIGW"
     gene            2078..2347
                     /locus_tag="PK442_04570"
     CDS             2078..2347
                     /locus_tag="PK442_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOO86952.1"
                     /translation="MVGSWRVVFTKAALKDAEKLLSAGLKPKAEQLLALLREDPYRSP
                     PPFEKLVGDLSGACSRRINIRHRLVYQVIETEHTVKVLRMWIHYE"
     gene            2510..3520
                     /locus_tag="PK442_04575"
     CDS             2510..3520
                     /locus_tag="PK442_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO86953.1"
                     /translation="MKRYGKLVCIATAALLAFAFLGGAAFAKELVVYSSVDEENAKNL
                     LDEFSKATGITVNMVFLSSGPAMSRIEAEQANPQADVWFGAPNENHIIAKDRGLTQPY
                     VSAAAGDLASGFKDPEGYWHAFYMNPLGFGVLPEELKADGKPVPASWKDLLDPAYKGM
                     IQMPSPQSSGTAYAMIMTLIETFGENEAYAYMKELNPNIQTYTQSGTGPSKNLAIRET
                     SIAIQFTPAFLKLVDEGYPAKVVFPAEGVGYEAAALSIIKGAKNLDSAKALVDWIVSK
                     EGQQVLSAKKTYFFPVRTDVSAGEGLPPLSEIKLIDYDRLEAAREKKRLVDRWVTEVL
                     GQ"
BASE COUNT          731 a         1133 c         1049 g          681 t
ORIGIN      
        1 aacggcgtat gcaggcatac ggaaattcga gagaggcctc ccggacggga agcctctctt
       61 ttcactttat cccggcatgg ggagaaggcg cgccttcccc ggttctttcg gtattcccat
      121 gcgcagcacc atgtcgaatg tgctgggctg gcaggtctgg cagatcgcgg aatcgcccct
      181 cacggaaagg taaagcgccg cgtccgtttc gtcccatccc caggccgccg cgatcagccg
      241 ctgcatctgg cgtgcggcct cccgctgtac agcgacgaac tccggaccgc tggcgagcgt
      301 atggatcatg tcgcctgtct ccaacatagg ccagtcgagc ggcagatttt tgatcaagtg
      361 cagccggaca agcaccgttc ccccgatttc gatccccgta ccgccgatct cgccgtcgcc
      421 catcgaagcg tgcagatcgc cgagctgaaa gagcgcgccc cgtgtccgaa cggggaaata
      481 cgccttggcg cccgcggtca tgaacttgca gtcgaggttg cctccgtgcg ctccgggctg
      541 gccgcagacc accgcgccct cggcgggagc cgttccgatg acgccgacca tcggacgcac
      601 cgggaagacg aggtcgttga agaacgcttt cccgtcgcgg acctcgatca ccttggtacg
      661 cagctcgcac cacccgatca gcggcccgga atccggcagc gtgcaggcga ttcccttttc
      721 cgcgacgtcg atggaaagaa tctccgcaca cagcgtatcc cccggctcgg cgccctccac
      781 gaagaccggg cctgtggcgg ggttcaccct gtcgaaatca atcgccgtca cgaggtcgga
      841 cgctcccgtc acctgatcgc agaaacagtc cttcgtcatg aagcgcacca tatccccggg
      901 actcacccgc gctacgggcg gatgttcggg cgaaaaggaa aagaccacgt cggaaactga
      961 aagcatggcg gaacctcctt ctggtgtgtt tcttcacatt gtagcaggga gaagcgcgca
     1021 aggaaaggaa cacgcggacg gggtcccccc gtacttcaac acaccgcgat ttccgtattc
     1081 tttctcttat tttcttccgg cttgcgccgc cgtgttcatt cgaccgacga cgtcgagtat
     1141 cttcccaacc tgccgttcat cctccgtacc gaggtatttg aacagcgccg tccggacgtc
     1201 ctccgaactc cgcatcgcgg cggagagggc gaacgccccc gccatcacgt tgttccgcgc
     1261 cttgaggaga tcgtccacgg tcttgatctt cgggaagtgc ttcgtgatcc aggccatgtt
     1321 ctcctgcacg ttcacctgca tcaggccgac ccgtgaatgc gaaacggcgt ccgcccgccc
     1381 ctccgactcc acgcagacca gcgcggcgac cagaaagggg tcgaccttgt agagttcgca
     1441 ggcgtcccaa atatgccgcg agtactccgt cgccttccgt attggcccct tgatcttcca
     1501 cggggtcatc gccgcgctga tctcgcgcga catgatgccg atggcggctt ttcttccgac
     1561 cgacgttccc gggatttctt gagcatagag ttttacagca aacactcccg cgagaaaaat
     1621 cgccgccgac aggatcgcgc acaaagcagc gccccatatt ccgtttcgca tgagcacacg
     1681 ctccttcgca tgttttcgag catgatagca tcgaagaaag aggaaggcat cgtgacaaat
     1741 acggcttctt gggaagagcg aacggtggtg acgctgcact ccggcgtttg acctcgccgc
     1801 agacatcatg taccatagaa cgtaccacag gaggtgaaga tatgagcgac atcacagcaa
     1861 cggaagcccg aaaacggctc tttcagcttc ttgacgaggt ggcggatacc cacgttccgg
     1921 tgcgcatcgc gggaaaacgc aacgcggccg tgctggtcag tgagtccgac tggagggcca
     1981 tggaagagac gctctttctg tgcagcatcc ccggaatgcg cgactccatc atcgaaggaa
     2041 tggcaacacc ggtggaagag tgcaccgagg atatcggatg gtaggatcgt ggagagtcgt
     2101 tttcaccaag gcggctctga aagatgcaga aaagctgctt tcggcggggc tgaaacccaa
     2161 ggcggaacag cttctcgcgc tactgcgtga agacccctac cgatcccctc cgccttttga
     2221 aaagctcgtc ggcgatcttt caggcgcatg ttcccgccga atcaacatcc gccatcgtct
     2281 ggtctaccaa gtgatcgaaa cggagcatac ggtaaaagtg ctccgcatgt ggatccatta
     2341 cgaatagcgt acaactacgg gagggtaccg ggctcctcgg aagtccgcag attggaaccc
     2401 ctaatccttt gtgctcctcg cttttcgctc ttctctttgt gttacaatac tgcaagattt
     2461 ccgcaacact tgggcagtcc tacactctga ttcgggaggg atttgaagaa tgaaacggta
     2521 cggaaaactg gtgtgcatcg cgaccgcggc gcttctggcg ttcgcgttcc tcggcggcgc
     2581 ggcgttcgcg aaggaactgg tggtctactc cagcgtggac gaggagaatg cgaagaacct
     2641 cctcgacgag ttctcgaagg cgaccggcat cacggtgaac atggtctttc tctcctccgg
     2701 tccggcgatg agccgcatcg aggccgagca ggccaatccg caggcggacg tctggttcgg
     2761 cgctcccaac gaaaaccaca tcatcgcaaa ggaccgcggc ctgacgcagc cgtacgtctc
     2821 cgcggcggcg ggcgaccttg cttccggctt caaggatccc gaggggtact ggcacgcgtt
     2881 ctacatgaat ccgctcggct tcggcgtgct ccccgaggag ctgaaagccg acggcaagcc
     2941 cgttccggcg tcgtggaagg atctgcttga cccggcgtac aaggggatga ttcagatgcc
     3001 ctcgccgcag tcgtccggca cggcctacgc catgatcatg accctgatcg aaacgttcgg
     3061 cgagaacgag gcgtacgcgt atatgaagga actcaatccg aacattcaga cgtacacgca
     3121 gagcggcacc ggccccagca agaacctcgc catccgcgag acgtccatcg ccatccagtt
     3181 caccccggcg ttcctgaagc tggtggacga ggggtacccg gcgaaagtcg tcttccccgc
     3241 cgagggcgtg gggtacgagg cggcggcgct ctccatcatc aagggggcga agaatctcga
     3301 cagcgcaaag gcgcttgtcg actggatcgt ctccaaggaa gggcagcagg tgctcagcgc
     3361 gaagaagacc tacttcttcc ccgtgcgcac cgacgtctcg gcaggcgaag ggcttccccc
     3421 gctctccgag atcaagctga tcgactacga ccgtcttgag gccgcgcgcg agaagaagcg
     3481 cctcgtcgac cgctgggtga cggaagtcct cggtcagtag ccgccgcgct ttcgacgaac
     3541 cgggggaagg ggctcgcctc ttcccccttt tgcttaggag gtgtcgatca acag
//
LOCUS       DAOSWW010000164         3589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18367_length_3589_cov_4.032824, whole genome shotgun sequence.
ACCESSION   DAOSWW010000164 DAOSWW010000000
VERSION     DAOSWW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18367_length_3589_cov_4.032824"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /gene="rplM"
                     /locus_tag="PK442_04580"
     CDS             complement(<1..367)
                     /gene="rplM"
                     /locus_tag="PK442_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOO86954.1"
                     /translation="MLGNRTYMATKEKVERKWYIVDATDKPLGRIASQVAKVLMGKNK
                     PTYTPHVDTGDFVIIINAEKAKLTGNKRQTSTVHHYTGWLGGFRSATYGEMMEKKPVQ
                     LMERVVKGMLPRNKLKFHRK"
     gene            complement(564..1589)
                     /locus_tag="PK442_04585"
     CDS             complement(564..1589)
                     /locus_tag="PK442_04585"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOO86955.1"
                     /translation="MESFSDVRDFRPEGGRLYSATHEEILGGCTTDVYFVKTRDVLRK
                     SGKLSTDVTAEIFARKNGVFAGLDEALLLLKGKDLAVESLREGDPFGPKETLMRIQGS
                     YELFGTYETVLLGMLASASAWATAAKACVEAARGKPVLCFGARHVHPSIAPVMERTAV
                     RVGGCSGASCILGAKLAGIEPVGTVPHAAVLIMGDTVDLAMAYDAEMPSGDPRIVLVD
                     TFKDEAEESLRVARALGDKLSGIRLDTPGERGGVTPDLVREIRYRLDMAGFSKVRIIA
                     SGGLSPERIRELDQAGADSFGVGSYISHADPIDMTMDIKEIDGEPVAKRGRLPGRMDN
                     PRLTRVV"
     gene            complement(1644..1868)
                     /gene="secG"
                     /locus_tag="PK442_04590"
     CDS             complement(1644..1868)
                     /gene="secG"
                     /locus_tag="PK442_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOO86956.1"
                     /translation="MKTFLGIVHILLCIALSGAILLQQRKQGGFSGIFGGGTQADMGG
                     SQWQRFTGLSKVTVILTAIFMVTSIVLVLL"
     gene            complement(1914..2513)
                     /gene="rdgB"
                     /locus_tag="PK442_04595"
     CDS             complement(1914..2513)
                     /gene="rdgB"
                     /locus_tag="PK442_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOO86957.1"
                     /translation="MRAPGLERLLFASGNRGKYVEVIDLFAPLGVDILYGPDVVVLDV
                     EETGSSYGSNAFLKAKAYAAASGMPALADDSGMEVRALNWEPGIFSARVAPTDGERIE
                     TILGRMRGVADRYARYVAAFALYFPSGSCIVTEGVCPGTLGEAPAGDRGFGYDPVFSP
                     SGYDRTFGELPDEVKRKISHRAVAGYRLMDILSGNCVVE"
     gene            complement(2524..3213)
                     /locus_tag="PK442_04600"
     CDS             complement(2524..3213)
                     /locus_tag="PK442_04600"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOO86958.1"
                     /translation="MGRKYDDFEDGFEVRRDEAPRSSRRSAANREGGRKAPLFIRLIA
                     WTGVLVFCFAAGYVGTSVALRMLNRQDILQREDVAANRGEAEKILNEAPKEIRLNARK
                     VEFTLYYPKDGTISSEKAEVLSGIMEDDIRQVIGKILSTVSGKGASDVKLLNLFRSGD
                     TLFLNFNAHFLSLLKAQGEQGSALLITSVVRTMNENFSPIVQVRFLVDGKEQKGGAPV
                     DLSVPWRLPRG"
     gene            complement(3235..>3589)
                     /locus_tag="PK442_04605"
     CDS             complement(3235..>3589)
                     /locus_tag="PK442_04605"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOO86959.1"
                     /translation="FENRELAEDNGDSTKAADKRTQTLLAILGNMQQNAKISESTGFA
                     EYLFNGGKAGGLKMRRVAQAPFYVLRGAAMPAVLVELGFLTDRQEAKLLADKSYQRKM
                     ADALAGGIREYLKKN"
BASE COUNT          732 a         1111 c          953 g          793 t
ORIGIN      
        1 gttttcggtg gaacttcagc ttgtttctgg gaagcattcc cttgacgacc cgttccatga
       61 gctgcacggg tttcttctcc atcatctcgc cgtaggtcgc cgaacggaac ccgccgagcc
      121 atcccgtata gtggtgcacg gtgctcgtct ggcgcttgtt gcccgtcagc ttcgcctttt
      181 cggcattgat gatgatgacg aaatcccctg tatcgacatg cggcgtgtag gtgggtttgt
      241 tctttcccat cagaaccttg gccacctggg atgcaatccg gccaagaggc ttatctgtgg
      301 cgtctacgat gtaccacttt cgctccacct tttctttggt ggccatatag gtgcggttac
      361 ccaacatggt atttcctcct tcaaaagctt cttttacaat tcaggcggtt ctttgatcta
      421 tgtaaaaatc atctcttctt caacgaatga aaaacgcttt ccgggggaaa cgtggctggg
      481 gtctcccagg gcgcatcttt tcatcgaaga aggagcgatc tggacaaaaa taccgtactt
      541 ccggctgaaa ccagaaatcc gatttacacc acgcgcgtca gccgcggatt gtccattcgt
      601 ccgggcagtc tgccgcgctt cgctaccggc tcgccgtcaa tctccttgat atccatcgtc
      661 atatcgatag ggtcggcatg agatatataa cttcctacgc cgaaggagtc cgcgcccgcc
      721 tggtcaagct cgcgtatacg ctccggagag agaccgccgg aagcgatgat acgcaccttg
      781 gagaatccgg ccatatcgag cctgtatcgg atctccctga caagatcggg cgtaacgccc
      841 ccccgttctc cgggggtatc gagacggata ccgctcagtt tatcgcccaa ggctcgggca
      901 acacggagcg actcctcggc ctcgtccttg aaggtatcga cgagaacaat cctcggatct
      961 ccggagggca tctccgcgtc gtacgccatc gccaaatcga cggtatcgcc cattatgagg
     1021 actgccgcgt gcggcactgt gcccacaggc tcgataccgg cgagcttcgc gcccagaata
     1081 cagcttgcgc cggaacatcc gcccacacgc acagctgtac gttccatcac gggcgcaatc
     1141 gaaggatgga catgacgagc cccgaaacag agtacaggtt ttccccgggc cgcttcgaca
     1201 cacgctttcg cagctgtcgc ccatgcggag gcgctcgcca gcattccaag gagaacggtt
     1261 tcgtacgttc cgaagagctc gtacgagccc tgtatccgca tgagggtctc tttcggtccg
     1321 aaggggtctc cctcgcggag ggattccacg gcgagatcct tccctttgag gagcaaaagc
     1381 gcctcgtcaa gcccggcgaa gacgccgttc tttcgtgcga agatctccgc ggtcacatcc
     1441 gtggacagtt ttccgctctt ccggagaacg tcccgagtct ttacaaaata gacgtcggtc
     1501 gtgcatccgc ccaggatttc ctcgtgtgtg gcggaataca aacgacctcc ttcaggacgg
     1561 aagtcacgga cgtcggaaaa actttccatc accggacaca ccggacagga ttgccctctg
     1621 ctgcgggatt ccatgggctt ctcttacaga agaaccagga cgatcgaagt taccatgaag
     1681 attgcagtca aaatcacggt gaccttggaa agccccgtga aacgctgcca ctgacttccg
     1741 cccatgtctg cctgggtacc tccgccgaaa ataccggaga aaccgccctg ctttctctgc
     1801 tgcagaagga tggctccgga cagagctatg cagagaagaa tgtggacgat accaagaaac
     1861 gtcttcaagt cgtgcacctc ctaaaaaaat gggaaccaaa tccaaacgaa aagctattct
     1921 accacacaat tccctgaaag gatatccatc aatcgatatc ctgccactgc ccggtgagaa
     1981 atttttcttt tcacctcgtc cggcaattcg ccgaaggtcc ggtcgtaccc ggagggcgaa
     2041 aagaccgggt cgtacccgaa cccgcgatct cccgcgggag cttctccgag cgttccggga
     2101 cagacgccct cggtcacgat gcagctgccg gaggggaaat agagcgcaaa ggccgccaca
     2161 taccgcgcat agcggtccgc cacgccgcgc atacgtccga gaatcgtttc tattctctct
     2221 ccgtcagtcg gtgcgacgcg ggctgagaaa atacccggtt cccagttcaa cgccctgact
     2281 tccatgccgc tgtcgtcggc cagcgcgggc attccggacg cagccgcata ggccttcgct
     2341 ttgagaaagg cgttgctccc gtagctgctc cccgtttcct cgacgtcaag gacgacgacg
     2401 tcgggtccgt agagaatgtc gacgccaagc ggcgcgaaaa gatcaatcac ttcgacgtac
     2461 ttgcctctgt tgccgcttgc gaagaggagg cgttccagtc cgggagctct catgtgcgcg
     2521 gcgtcatcct ctcgggagac gccacggcac gctgaggtcg acgggcgcgc cgcccttctg
     2581 ctccttgccg tcgacgagga agcgcacttg aacgatcggc gaaaagtttt cgttcatcgt
     2641 ccgcactaca gaggtgatga ggagcgcgct cccctgctct ccctgcgctt tgagcagcga
     2701 aagaaagtgt gcgttgaagt tgagaaagag cgtatcgccc gagcggaaga ggttgagcag
     2761 cttgacgtcg gaggctccct ttcccgaaac cgtcgacagt atcttcccta tcacctggcg
     2821 gatgtcgtcc tccatgatac cggagagcac ctccgccttc tcggacgaga tcgtcccgtc
     2881 tttcggatag tacagcgtaa actccacctt gcgggcgttc aggcggatct ctttgggcgc
     2941 ctcgttcagt attttttcgg cttctccgcg gttcgccgct acatcctctc tttggaggat
     3001 atcctggcgg ttgagcatgc gcaacgcgac ggaggtgccc acataccccg ctgcaaaaca
     3061 gaaaaccagt acgccggtcc acgcaatcag tcggatgaag agcggcgcct tccgtccgcc
     3121 ttctctgttt gcggcagatc gcctcgagga acggggagcc tcatccctcc gcacttcgaa
     3181 accgtcttcg aaatcatcat actttctccc catcaaatga tcgtcctcct tggatcagtt
     3241 ctttttcaga tattcccgta tcccgcctgc gagagcatcc gccatcttcc gttgatagga
     3301 tttgtcggcg agaagcttcg cctcctgcct gtccgtcaaa aagcccagtt cgacgagcac
     3361 ggcgggcatc gccgcccccc tgagcacgta gaacggcgcc tgggctacac ggcgcatctt
     3421 gagtcctccc gcttttccgc cgttgaagag atactcggca aacccggtgc tctcgcttat
     3481 cttcgcattc tgctgcatgt ttccgaggat ggccagaagc gtctgggtcc gtttgtccgc
     3541 ggccttcgtc gaatcgccgt tgtcctcggc caactccctg ttttcgaag
//
LOCUS       DAOSWW010000165         3586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18391_length_3586_cov_3.421410, whole genome shotgun sequence.
ACCESSION   DAOSWW010000165 DAOSWW010000000
VERSION     DAOSWW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18391_length_3586_cov_3.421410"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..946
                     /locus_tag="PK442_04610"
     CDS             167..946
                     /locus_tag="PK442_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO86960.1"
                     /translation="MGRFFTAFLLLVLLGSTACVSVASAGETVVRITNGDWPPFLGEH
                     LPENGAISQVVREAFALKGIETRYGFFPWARALALAAEGKWDASVGWAFSEERARDFF
                     FSDALVDLPVLLFSLNEKKVEWKEWQDLAGLLIGVTRGYHYGNEFEKASEEGLFRTDP
                     SSDDETSLRKLLGKRVDAVAVDRYVGIELINRGFALEADRFWYCETPVRSAPYSLLFS
                     KKAPNAEELLAAFNEGLAELKASGRYQEILDDAASGKYSTP"
     gene            complement(1269..2018)
                     /locus_tag="PK442_04615"
     CDS             complement(1269..2018)
                     /locus_tag="PK442_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO86961.1"
                     /translation="MEFAGRTALVTGAGTGNGEAIAERLFTGGASVGLVSRRLESVDA
                     VCRRIDPEGERTLAIEADVRDPKAMEAAVQRIVERFGSLDLAVNNAGITGPAGAPVQD
                     IESETWLEVIATDLTGVFYSMKYEIPAMLANGSGAIVNMSSANGLVGLPGMAAYTAAK
                     HGIIGLTRSAALELAESKIRVCAVAPGYVATPRILDSGKEVTDWMAGVHPMKRLAARE
                     EVADLVAFLLSDSASFITGSVHSIDGGYTAQ"
     gene            2338..3213
                     /locus_tag="PK442_04620"
     CDS             2338..3213
                     /locus_tag="PK442_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOO86962.1"
                     /translation="MNEFSFFATQGPLSRPGAHAELFDSLPADIGELCAVVQGVTVHP
                     FWAERYGVTLAAERGAEVQLRTMEKRLARTLELDSRPLTVPRPLELKTVGNCRDHTLL
                     LVSMLRSQGVPARARCGFGTYFLPGHFEDHWVAEYWNESEKRWILVDAQLDAFQRGVL
                     NVSFDPLDVPRDRFIGGGAAWRMCREGVADPNAFGIFDMRGMGFVGSNLVRDVAALNK
                     VELLPWDCWGMMLERDTDDSDLALLNRLAELTEGDVPDFGAVCELYESDPRLKAGATI
                     QSFVQGEMKVVELEL"
BASE COUNT          695 a         1058 c         1043 g          790 t
ORIGIN      
        1 catcatgtct taaatttctt ccgaaaaaaa cgagcccgct gaaggggtgc ttgacaccca
       61 taacatttcg gcctacaatt tcaagaaatc aatacaaatt gtatttttta gtgctatttt
      121 ccatttgcga atttcgcatt ataccatcac gcgggaggtg ttgctcatgg ggcgattttt
      181 cacagcgttt cttcttctcg ttcttcttgg ttcgactgcc tgcgtctccg ttgcttcggc
      241 cggagaaacg gtcgtccgca ttacgaacgg cgactggcct ccgtttctgg gggagcatct
      301 tcccgaaaat ggagcgatct cgcaggttgt gagggaagcc tttgccctta agggcatcga
      361 aacgaggtat ggattcttcc cgtgggcgcg cgctcttgct ctcgccgcgg aggggaagtg
      421 ggacgcttcc gtcgggtggg ctttttcgga agaacgagcg cgggactttt tcttcagcga
      481 cgcgcttgtc gatctgccgg tgcttctctt ctccctgaac gaaaagaagg tcgagtggaa
      541 ggagtggcag gaccttgcag ggctcttgat cggagttacg cgggggtatc actacgggaa
      601 cgagttcgaa aaagcgagcg aagaaggtct cttccgaacc gatccatctt cggacgacga
      661 gacgagcctc aggaagctcc tcggaaaaag agtggatgcc gtcgcagtcg acagatatgt
      721 aggtatcgag ctgatcaacc gcggatttgc cctggaagcc gaccggttct ggtactgcga
      781 aacccccgtc cgctcggcgc cgtactctct gcttttttcg aaaaaagcgc cgaacgccga
      841 ggaattgctg gcagcattca atgaggggct cgccgagctg aaggcaagcg gccgatacca
      901 ggagatactg gacgacgcgg cgtccggcaa gtattcgact ccgtgacgtt ttcgcccgta
      961 cgagccgtgg agacgacgga acgactctac aatcatcggt ttggcatttt ggaaccgttg
     1021 cgttacgttc ttcaccggtt actcgtgaag ccacggggcg tagctcaagc cgatcaacac
     1081 cccttcgggg ctgatgaacc gcgcgacggt ctgcccccac ggttccgtcc gcgcctcgtg
     1141 aacgaattca cgccgccggg tctttcgtaa tgacggcgaa acccgctacg ctctcgattt
     1201 ttatcggtcc gttcattgta tttcctcctt ttgtactcat agagtcgaac gaggtgtatg
     1261 tgatcacatt attgcgcggt gtacccgccg tcgattgaat gaacgctccc ggttatgaag
     1321 gacgcgctat cgcttaacaa gaaggcgacc aaatcagcga cctcttccct tgcggcgagg
     1381 cgcttcatgg ggtgcacccc cgccatccag tccgtgactt ctttgccgga atccagtatc
     1441 cgcggggttg cgacgtaacc cggcgcgacg gcgcatacgc gtatcttgga ttcggccaat
     1501 tcgagcgccg cggagcgggt gagcccgatg attccgtgtt tggccgctgt gtacgcagcc
     1561 attcccggca agcccaccaa gccattggcg gacgacatgt tgacgatcgc gccggacccg
     1621 tttgcgagca tcgcgggtat ctcatacttc attgagtaga agacgcccgt cagatcggtt
     1681 gcgatcactt ccaaccacgt ttccgattct atgtcctgaa ccggcgcccc ggccggaccg
     1741 gtgatccccg cgttgttcac ggccagatcg agcgagccga agcgttcgac gatgcgttgc
     1801 accgcagcct ccatcgcctt gggatcgcgc acatcggctt cgatagccag cgtccgttca
     1861 ccttcagggt caatccgccg gcagactgcg tccactgatt caagacggcg actcaccagc
     1921 ccgaccgacg ctccgccggt gaagagccgc tcggctattg cttcgccgtt ccctgttccg
     1981 gcgccggtaa ccagcgctgt tcttcctgca aattccatat cgcacctcca ggctgcgtca
     2041 ggtgtttcct cattgttgca cgatggttat gccatgactc ccaatcattc tgcttcttga
     2101 caataaaact tctttagaaa atagtaccac gaatggcgcc ttctcggaga cctcaccctc
     2161 cgtttttgaa agagggccgg gtggtgtctg caccgaggcg ccgggccgag gcaactgata
     2221 caatacatac cgtcggacgg gagtcgatcc ccgttcccga atactgttag acgcgatccg
     2281 ccggacgctg cggcgttcga agcggggtac gccgctctct gtttaaggag gaaacctatg
     2341 aacgaattct cgtttttcgc gactcaaggg cctctcagca ggcccggagc gcacgctgaa
     2401 cttttcgatt ctctccctgc ggacatcggg gagttgtgtg cggtcgtcca gggcgtcacc
     2461 gttcacccct tctgggccga acggtacggg gtgacgctcg ccgccgagag gggcgccgag
     2521 gtgcagctcc gtaccatgga gaaacgtctg gcccgcactc tggaactcga ttcgcgccca
     2581 ctgaccgttc ctcggccgct ggaattgaag acggtcggca actgccgcga ccacacgctg
     2641 ctgctggtgt ccatgctgcg cagtcaaggg gtcccggccc gcgcgcgctg cggattcggg
     2701 acgtacttcc tgcccggaca cttcgaggat cactgggtcg ccgaatactg gaacgaatcc
     2761 gagaaacgct ggatactggt cgacgcgcag ctcgacgctt tccagcgcgg cgtgctgaac
     2821 gtttcattcg atccgctgga cgtgccccgg gaccgcttca tcggcggcgg cgcggcctgg
     2881 aggatgtgcc gcgagggggt tgcggacccg aacgccttcg gcatcttcga catgcgggga
     2941 atgggtttcg tgggaagcaa tctcgtccgc gacgtcgcgg cgctcaacaa ggtcgaactg
     3001 ctgccgtggg actgttgggg gatgatgctc gaacgcgaca cggacgactc cgacctggcg
     3061 ctgctcaatc gtctggccga gctgaccgag ggcgatgttc cggacttcgg ggctgtatgc
     3121 gaactctacg agtcggaccc gcgcctgaag gccggcgcga cgatccaaag tttcgtgcag
     3181 ggcgaaatga aagtggtcga gctggaactc tgaaacgcat ccatcgtggg aggacgggag
     3241 ggagcctctt cacgcaggcg cgcatcctct atccgacgga cgaggggttc cgagtacatc
     3301 cttgaagtga aagacggacg gaaagcggct tttttcggta gtattcttgc ctcgaagcat
     3361 tcccgagact ccatgcgccg ctctccgtct ccgccgaaag cctcaaaaac ctcacatcgc
     3421 cgaagaattt tttgcaggcg aagcgcaacg agacgttttt tcaaccagac gaatttcgac
     3481 gtcgcaccct actgcttttg catacttcct cagcgtctgg acggaaggag aatgctttcc
     3541 ccccgcttcc aggcgggaaa ctgcgctttt acttgttccc atggac
//
LOCUS       DAOSWW010000166         3585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18403_length_3585_cov_3.937960, whole genome shotgun sequence.
ACCESSION   DAOSWW010000166 DAOSWW010000000
VERSION     DAOSWW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18403_length_3585_cov_3.937960"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1110
                     /gene="era"
                     /locus_tag="PK442_04625"
     CDS             166..1110
                     /gene="era"
                     /locus_tag="PK442_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOO86963.1"
                     /translation="MNNPFAGIGFRAGNVVLAGRPNVGKSSLINRLLDYKVSIVSEKP
                     QTTRNVIRCVYNGEDFQIIFSDTPGIHLPKHKLGRTLVDAAVESLEEADLVCYVVEAE
                     DRTVAPEDEEILSVLGKVRTPVLLVVNKCDVVLDRKGGGEAALAAVRSIYEARISLCG
                     VVFVSARMGRGLDTLIAAIRPHLPEGPPFYDEDILVDRPEKFIAAEIIREKVLLMTHR
                     EVPHSVAVEIEEYKSPDEYPERDVLFIRATLYVEREGQKRIVIGDSGARLKEIGRRAR
                     MDIETLTGHKVYLDLWIKVRKDWRQSDADLRMLGIQER"
     gene            1116..1913
                     /locus_tag="PK442_04630"
     CDS             1116..1913
                     /locus_tag="PK442_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOO86964.1"
                     /translation="MRAADIFVSSGGEYQDIPNRRSFPSYSGDSIPQGLVRLSGVVLR
                     RDVSAEGNVSLYLFLKETGPVWVSAPGASRGRVRFGGAIEPLVWANFNLYKGARRFYL
                     QSAEVKEDFWSLRSDSGKIRRMLEWDGLLCEHLVPGLPCDDVLALFYWSGVLLKEDVH
                     PLVAEWRFLWKWLYNWGIAPSPDLCGECGAALSSARLTASGFLCPACARTHEGTALSG
                     DELSLLRRSLEIPVKKMIEYFPSKPFPGDSPWKEVNGRLRLYFSMMQ"
     gene            1972..2859
                     /locus_tag="PK442_04635"
     CDS             1972..2859
                     /locus_tag="PK442_04635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOO86965.1"
                     /translation="MNFQEIVFRLERFWAEQGCVVQQPYDIEVGAGTMNPATCLRVLG
                     PEPWRVAYVEPSRRPTDGRYGENPNRLQHYYQYQVIIKPAPSNIIDLYLQSLAALGIE
                     PEAHDIRFVEDDWESPTVGAWGLGWEVWLDGMEVTQFTYFQQVGGIDMDLVPAEITYG
                     IERIAMFVQKVENVYDLQWVDNVKYGDVHHKGEVEYSHYNFELADTKMLFQLFGMYEA
                     EADRVLAKGFVLPAYDYVLKCSHTFNLLDARNAISVTERTGYIGRIRMLASRCCAAYA
                     EQRREMGLPFTGKFDETKR"
     gene            2865..>3585
                     /locus_tag="PK442_04640"
     CDS             2865..>3585
                     /locus_tag="PK442_04640"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOO86966.1"
                     /translation="MTYKNVLLEIGTEEIPSRFLPDTLEFLEKTAKDDFKNARIGFKS
                     LSVFATPRRIALMVKDVDDQQQDLVNTFKGPAWTAAFDMNGNPTRAAFGFAKSKGVSI
                     EQLVGVEVDGVKYAHAEVSEPGKPVLEILPELLPALIKKIVFPKNMYWNIPSVRFARP
                     IRWILAMADTNVIPFQYGGIESGSTTRGHRFMGAKQIVVDDAANFMERLYDNHVILDQ
                     EKRRQKMIAGISALENDLDGAV"
BASE COUNT          762 a         1039 c         1079 g          705 t
ORIGIN      
        1 aaaaaggggt gaggtttgtc tgccgtgcgg cgcgtgccgg cagttccttg cggagttcaa
       61 tccggaaatg ctgctagtct ttgaacaggg agattcgttc gtggcgacca ctttagcgga
      121 gcttttcccg gccccgttcc ttctgacgga gcagagcggg gaacgatgaa caaccccttc
      181 gcagggatcg gcttccgtgc ggggaacgtt gtcctcgcag gccgcccgaa cgtcgggaag
      241 tcctccctga tcaaccgact tcttgactac aaggtttcca tcgtttccga gaaaccgcag
      301 acaacgagaa acgtcatccg ttgcgtctac aacggggaag actttcagat catcttctcc
      361 gacactccgg ggatccatct cccgaagcac aagctgggca gaacgctggt ggatgcagcc
      421 gtagaatcgc tggaggaggc cgacctcgtc tgttacgtcg tcgaggcgga ggacagaacc
      481 gtcgcccccg aggacgaaga aatcctctcc gtcctcggaa aggtgcgcac ccccgtgctc
      541 ctcgtcgtga acaagtgcga cgtcgttctc gaccgaaaag gaggagggga ggcggcgctt
      601 gccgccgtgc gctccatcta cgaggcgcga atctctctct gcggggtcgt cttcgtctcc
      661 gccagaatgg gaagagggct cgatacgctt atcgccgcaa tccgtcccca ccttccggag
      721 gggcctccct tctacgacga ggatatcctt gtcgaccggc ccgagaagtt catcgccgcg
      781 gagatcatac gggaaaaagt gctgctgatg acgcaccggg aggtgccgca cagcgtcgcc
      841 gtcgaaatag aggagtacaa aagccctgac gaataccccg agcgcgacgt gctttttatt
      901 cgcgccacgc tctacgtgga gcgcgaaggg cagaagcgca tcgtcatcgg cgattccgga
      961 gcgcggctga aggagatagg cagacgggcg cggatggata ttgaaacgct caccggccac
     1021 aaggtctatc ttgacctctg gatcaaggtg cgcaaagact ggcgtcaatc cgacgccgat
     1081 ctgcggatgc tcggcattca ggaaaggtga cgggggtgcg tgcagcggat attttcgttt
     1141 cgtccggcgg ggagtaccag gacattccga accgccggtc ctttccgtcc tatagcggcg
     1201 attccatccc ccaggggctc gtcagactgt ccggggttgt gctccgcagg gacgtctccg
     1261 cagagggaaa cgtctctctc tatctctttc tgaaggagac ggggccggtc tgggtctctg
     1321 cgccgggagc gtcgagaggg agagtccgtt tcggcggcgc catcgagccg ctcgtctggg
     1381 cgaatttcaa cctgtacaag ggggcgcgcc gcttctacct tcagtccgcg gaggtaaaag
     1441 aggacttctg gagcctgagg tccgattccg gaaaaatccg gaggatgctc gaatgggacg
     1501 gactgctctg cgaacacctc gtacccggac tcccctgcga cgacgtgctt gcgctctttt
     1561 actggtcggg cgtgctgctg aaagaagacg tccatccgct tgtcgcggag tggcgcttcc
     1621 tgtggaagtg gttgtacaat tggggcatag cgccttcacc cgatctttgc ggcgagtgcg
     1681 gcgccgcgct ctcttcagcc cgtctgacgg cttccggatt tctctgcccg gcctgcgccc
     1741 gtacgcacga agggacggcg ctttccgggg atgaactctc cttactgcgt cggtcgctgg
     1801 aaattccggt gaaaaagatg atagaatatt ttccctcgaa accgtttccg ggagactcgc
     1861 cttggaagga agtgaacggg cgtctgcgcc tgtactttag tatgatgcaa taatagtgga
     1921 aaaagaatag agcttccggc agccggaagt ttttctcaag gagggatata tgtgaatttc
     1981 caggaaattg tctttcgcct cgaacgcttc tgggcggagc aggggtgtgt tgtacagcag
     2041 ccgtacgaca tagaggtcgg ggccgggact atgaatcccg cgacctgcct tcgcgtcctc
     2101 gggcccgaac cgtggcgcgt cgcctacgtc gaaccctcgc gccgtccgac cgacggccgc
     2161 tacggagaga acccgaaccg gcttcagcac tactaccagt atcaggtgat catcaagccc
     2221 gccccctcca acatcatcga tctctacctt cagagcctcg cggctctcgg gatagaaccg
     2281 gaggcgcacg atatacggtt tgtcgaggac gactgggagt cgcccaccgt cggcgcgtgg
     2341 gggctcgggt gggaggtctg gctcgacggc atggaggtga cgcagttcac ctacttccag
     2401 caggtcggcg gcatcgacat ggaccttgtc cccgcggaga taacctacgg gatcgaacgc
     2461 atcgccatgt tcgtccagaa ggtcgaaaac gtctacgatc tccagtgggt ggacaacgtg
     2521 aagtacggcg acgtccacca taagggcgag gtcgagtact cgcactacaa cttcgaactt
     2581 gccgacacga agatgctctt ccagctcttc ggaatgtacg aggcagaagc cgacagggtg
     2641 cttgcaaaag gattcgtcct tcccgcgtac gactatgtgc tcaagtgttc gcacactttc
     2701 aacctgctcg acgcgcgcaa cgcgatcagc gtgaccgaga ggacggggta catcggaaga
     2761 atacgcatgc tcgcaagcag atgctgcgcg gcctacgccg agcagcgccg ggagatgggg
     2821 cttcccttca ccggcaaatt cgacgagacg aagaggtgag gaacatgacc tataaaaacg
     2881 tactgctcga aatcgggacc gaggaaattc cctcacgttt cctccccgat acgctcgaat
     2941 tcctcgaaaa aacggcgaag gacgacttca agaacgcccg cataggcttc aagagtctct
     3001 ccgtctttgc gacgccgcgc cgaattgcct taatggtgaa agacgtcgac gatcagcagc
     3061 aggacctggt gaacacgttc aaaggaccgg catggacagc cgcgttcgac atgaacggaa
     3121 acccgacgcg cgcggccttc ggctttgcca agagcaaggg cgtctccatc gaacagcttg
     3181 tcggcgtcga ggtggacggc gtcaagtacg cccacgccga agtttcggaa cccggaaagc
     3241 cggtgctgga aatccttccc gaacttcttc cggcgctcat taaaaaaatc gtctttccaa
     3301 agaatatgta ctggaacata ccctccgtcc gctttgcgcg ccccatccga tggattctgg
     3361 caatggccga taccaacgtc atcccgtttc agtacggtgg aatcgagagc ggttccacga
     3421 ccagggggca ccgcttcatg ggcgcaaagc agatcgtggt ggacgacgcg gcgaacttta
     3481 tggagcgtct ctacgataac cacgtgattc tcgaccagga aaaacgccgt cagaagatga
     3541 tcgcaggtat cagcgcgctc gaaaacgacc ttgacggtgc tgtcg
//
LOCUS       DAOSWW010000167         3573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18498_length_3573_cov_3.190449, whole genome shotgun sequence.
ACCESSION   DAOSWW010000167 DAOSWW010000000
VERSION     DAOSWW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18498_length_3573_cov_3.190449"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..822
                     /locus_tag="PK442_04645"
     CDS             124..822
                     /locus_tag="PK442_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO86967.1"
                     /translation="MLSLEALSRETGESVREWAFRTILCNLVNLVLLPGAAVSENDVA
                     SALGISRTPVRETFIRLAQEGLLDVYPQKGTYVSLVDEQRVEEGRFMRLCLEGRTVEL
                     ACEEFSSESMVALRANLQLQRLAISEKNASRFFALDEEFHRIIFATCGKERIWEAIRK
                     MGSHFNRVRVLSLLATDWEKVFSQQERIASSIFEKKIGEARDAMNEHLTKVNYDLGRL
                     RQEYPSYFKTEKGR"
     gene            922..1905
                     /locus_tag="PK442_04650"
     CDS             922..1905
                     /locus_tag="PK442_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO86968.1"
                     /translation="MKKIALLIAVALLASLVFSTAALAEKKPEFTLKLGHLANTEHTW
                     HKGSVKFAELVEEKTGGRVVVQVFPSEQLGNEMEVIGNIQSGIVDMLITGESMMNWAP
                     LCGLIAVPYMIRDSAHMEKIVEGEVGQAIEKEVIEKVRLRPVAWFERGARNLTSNREV
                     KKPEDLNGLRLRVPNVPLFVATWESLGAKPTPMAFSEVFTSLQQGIIDGQENPYDLIK
                     SASFFEVQKFVNKTEHVRGWIYLVIGEDKFQSMPEDIRKAVLEAGKEAQAYERQLFRA
                     EQDQLIKDLQDKGMTFVEVDKEAFMKKTEPAVLSFLSPELQEMYKKIVAIQ"
     gene            1984..2463
                     /locus_tag="PK442_04655"
     CDS             1984..2463
                     /locus_tag="PK442_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO86969.1"
                     /translation="MKKPFNLFTRIMEFCVIVTFLGLVAVVLLQVYARLFLPRSPHWT
                     EEASRFLFAYTVSFAAGLAVKEKAYVNVDVFTNLLPEMLKQWLEVLLDLLVAAFMAVV
                     FYYSLKNLGIGRMRTSPSLTIPMHYIFASMSVMSGSIVLYSLAEVIRDTRALLKGGN"
     gene            2474..3496
                     /locus_tag="PK442_04660"
     CDS             2474..3496
                     /locus_tag="PK442_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO86970.1"
                     /translation="MLFIVFLILLFAGLPIAFCLGISSLVYLWMAGIPLIVIPQKMYS
                     GIDVFVLLCIPGFILAGNLMNRGGISDRIIRFCNALVGHIQGGLAQANIVASMIFAGI
                     SGTAVADTASLGAILIPAMKKEGYDAEFSCAVTAASSTIGPIIPPSMPMIIAGTLTGL
                     SVGRLFLAGAIPGVLLGLGLMVVSWFISKKRNYPKGVKVPRRLLLKEFFGAFWALMLT
                     VLILYGIVGGFFSPTEAAMVACVYAVVIGMFVYKDLTLKELPAVILETAITTASIIVL
                     VGLANLFAWILTSEQIPQMVADLLLSITSNKYLIILLINFLLLFVGRSWRRSPRCSRS
                     SRRSSP"
BASE COUNT          732 a          994 c          987 g          860 t
ORIGIN      
        1 gggtgcgccg ggaaatcctt cttgacaagg aacggctcct gcgggatagt aagaacggga
       61 agacgttctt aaccttttct tgtagaaacg catcttgtat actagtattc tggaggaggc
      121 cgtatgcttt cgctcgaagc gctgtcgaga gaaaccggag agagtgttcg tgaatgggcg
      181 tttcggacca ttctctgtaa tctcgtgaat ctcgttctgc ttcccggcgc cgccgtttcg
      241 gagaacgacg tcgcctccgc tctcggcata agcaggactc cggtgcgtga gacgttcatc
      301 cgcctggcgc aggaaggcct cctcgacgtc tacccgcaga aaggaacgta tgtctcgctt
      361 gtggacgaac agcgggtgga agaggggcgt tttatgcgtc tctgccttga agggcgtacc
      421 gtcgagcttg catgcgaaga gttttcatcc gaaagcatgg tcgcgcttcg ggcgaatttg
      481 cagctccaac gtctggccat aagcgagaag aacgcgtcac gatttttcgc cctcgatgaa
      541 gagttccacc gcatcatctt cgcgacctgc gggaaggagc gcatctggga ggcgatccgg
      601 aagatggggt ctcatttcaa ccgggttcgt gtgctcagtc tcctggcaac ggactgggaa
      661 aaagtgtttt ctcagcagga acgcatcgcc tcctcgattt tcgaaaaaaa aatcggtgaa
      721 gcgcgcgacg cgatgaatga acatctcacg aaggtgaatt acgacctcgg aagactccga
      781 caggagtacc cgtcctattt caagaccgaa aaagggaggt gagcgccggc cggcagcgta
      841 cgatttgttt cgcggcgtga atcgcccccg gttcgttcgg gggaagacct ggtatcgtat
      901 tgatgaaagg agattgttcc gatgaaaaaa atcgcactct taatagcagt tgcgcttctg
      961 gcgtccctcg ttttctctac cgccgcactg gcggagaaga aacctgagtt cactttgaaa
     1021 ctcggccacc ttgccaacac cgagcacaca tggcacaagg gatccgtcaa gttcgccgaa
     1081 cttgtcgagg aaaagaccgg cggccgcgtt gtcgtccagg ttttccccag cgagcagctt
     1141 ggaaacgaaa tggaagtcat cgggaatatc cagtccggca tcgtcgacat gctcattacc
     1201 ggagagtcca tgatgaactg ggctcctctg tgcggactta tcgccgtccc gtacatgatc
     1261 cgcgactctg cgcacatgga gaagatcgtg gagggcgagg taggccaggc aatcgaaaag
     1321 gaagtcatcg agaaagtccg ccttcgtccc gtggcgtggt tcgagcgcgg cgcccggaac
     1381 ctgacttcaa accgcgaagt gaaaaaaccc gaagacctga acggcctgag acttcgcgtc
     1441 ccgaacgtcc ctcttttcgt ggctacctgg gagtctctcg gcgccaagcc cactcccatg
     1501 gcgttctccg aagtgttcac ctcgttgcag caggggatca tcgacggtca ggagaacccc
     1561 tacgacctga tcaagagcgc cagcttcttc gaggtccaga agtttgtcaa caagacggag
     1621 cacgtccgcg gatggatcta tctcgtaatc ggtgaggaca agttccagag catgcccgaa
     1681 gatatccgga aagctgttct tgaagccggc aaggaagcgc aggcctatga gcgccagctt
     1741 ttccgcgccg aacaggatca gctgatcaag gatctgcagg acaaggggat gacctttgtc
     1801 gaagtcgaca aggaagcctt tatgaagaag accgagccag cagtgctcag cttcctctct
     1861 cccgaacttc aggagatgta caagaagatc gtcgctatcc agtagcacta tttcttccgg
     1921 gccccggtct tccgggagcc cggattcctt tttctttttt tgagagacga gggcgaccgt
     1981 catatgaaaa aaccattcaa tctcttcacc cgtatcatgg agttctgtgt aatcgtcact
     2041 tttctcggcc ttgtggccgt ggttcttctc caggtgtacg cccgcctctt tcttccccgc
     2101 tctccccact ggacggagga ggcttcccgt tttttgttcg cgtacacggt gagcttcgct
     2161 gcgggattgg ccgtaaagga aaaagcgtat gtgaacgtgg atgtcttcac caatttactt
     2221 ccggagatgt tgaaacagtg gcttgaagtt ttgctcgacc ttcttgtcgc ggcctttatg
     2281 gctgtcgttt tttactactc gctgaaaaac ctcggaatag gcaggatgcg gacgtcgccc
     2341 tccctgacca tccccatgca ctatatattc gcctccatgt ccgtcatgtc gggttccata
     2401 gtgctctact cgctggcgga agtcattcgg gatacgcgtg ctctattgaa ggggggcaac
     2461 tgacgtggtc gcgatgcttt ttatcgtctt tctcattctt ctctttgcgg ggctccccat
     2521 cgctttctgc cttggtatct cttctctggt ctatctctgg atggcaggca ttccgctcat
     2581 agtgattccg cagaagatgt actcggggat cgatgtcttc gtactgctct gtatcccggg
     2641 attcatcctt gcggggaacc tgatgaaccg cggcggtatc tccgaccgga tcatccgctt
     2701 ctgtaacgcg ctcgtcgggc acatccaggg cggactcgcc caggccaaca ttgtggcttc
     2761 gatgatcttc gcaggcatct ccggaaccgc tgtcgcagac accgcaagcc tcggcgccat
     2821 tctcatcccg gcgatgaaaa aagaggggta cgacgccgag ttctcctgcg ccgtcaccgc
     2881 agcatcctcg acgatcggtc cgatcattcc gccgagcatg ccgatgatca ttgcgggaac
     2941 gctcaccggc ctctccgtcg gccggctctt ccttgccgga gccattccgg gggtattgct
     3001 ggggcttggg ctgatggtgg tctcctggtt tatatcgaag aaacggaact accccaaggg
     3061 agtgaaagtg ccccgccgac tgctgctgaa ggagttcttc ggcgccttct gggcgttgat
     3121 gctcacagtg ctcatcctct atggcatcgt cggaggtttt ttcagtccca ccgaggctgc
     3181 gatggttgcc tgtgtctacg ccgttgtgat cggcatgttc gtctacaagg atctcacgct
     3241 caaggagctt cctgcggtga ttctcgagac cgcaatcacg acggcatcga ttatcgtgct
     3301 tgtcggcctg gcgaaccttt ttgcctggat tctcaccagc gagcaaattc cccagatggt
     3361 ggccgatcta ttgctgagta tcacgagcaa caaatattta attatactgt tgatcaactt
     3421 cctgctcctt tttgtaggac gttcatggag acgatcgccg cgctgctcac gctcttcccg
     3481 acgctcctcg ccgtagcgct gcgtgtgggg atagacccga ttcattttgc tatgatcgcc
     3541 gtgctcaatc tgatgatcgg attgaccacc ccc
//
LOCUS       DAOSWW010000168         3554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18647_length_3554_cov_3.498428, whole genome shotgun sequence.
ACCESSION   DAOSWW010000168 DAOSWW010000000
VERSION     DAOSWW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18647_length_3554_cov_3.498428"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..3548
                     /locus_tag="PK442_04665"
     CDS             204..3548
                     /locus_tag="PK442_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOO86971.1"
                     /translation="MKKTFFRSSHKRAASLADILVVVLLLAAGFFLGEWRVSKAELGM
                     RHNLVRRARLFAESVNPRAVKRLSGSESDRVSPNYRRFKEQSVSLLSAFPDCRFISLL
                     GRRADGSIFIYFDSEPEGSPDESPPGERYVPPDPVLLEAFDKGKSVVAGPFMDIWGSL
                     VAAWVPVADPDTGKPAFVAALDIDGGKWRSDARRAAYVPECAAVLLAALFIVWRILTL
                     RRALRRRTSYRWYHRYADALFVAATGLVLTFSILLVSSEVERSTREDTFHMLADSESG
                     KLHNSLKSIRDIGVEGAAAFFSYSDFVDFEEFKGYARHLEELPEVRGATWLPLVPHEE
                     REAFERLGRSLVTEDFVIRDYGEGGRRGPAPERPFYYPVFYRIPFEGSERILGADFAQ
                     VPSRRAAIDEARASRLPAATEAIPLLAAPGTLKGIVVFQPVFSRNETENFMGVLAIVV
                     ELNALLNRMRTEEAHVERAVLAGDLWRLYGAGVHEHVVSTGEGAILPPAWPSVGDLFS
                     VRPLLFFGRTFAVILRPGPGFRALYPPRAGWMSLFAGIMVTAVLTMLAVTVANRREEL
                     ERQVAMRTDELRRSKDELFLMNAALVDTTRKTQEIARRAEAASEAKGRFLANMSHEMR
                     TPLNGFLGMNRLLLDTRLTEEQREYAVAARKSGEALLTLISDALDIATIDEGRYRLVE
                     EDFDLDELLSKLETIFSAAAHEKGLRFTSVRPSVPLRLHGDGRCLYRILRSLLDNAVK
                     FTVRGEVRFSVSALEESDRTVRLRFSVEDTGVGIPSEQQGCVFERFWQGDDSTTKSYQ
                     GAGLGLPMAKELAALLGGELEMQSEEGRGTTFSFESLFHKGASPAAPSIQDESVTGRR
                     ILLAEDNPVNRKFALAMLRKLGYSVASAENGKEAVDYLSSNECDLVVMDVQMPVMDGV
                     EATRMIRSGASGALDPSIPVVALTAYVSPEDRERCMVAGMNAFLPKPAAPEELDAAIR
                     RLLYGEAGGERAETHVPAEYAGKAAPVFDRAAALKRLMGDEDALAELAEEFIEEMPKD
                     LEKFSALVSEGDAAAAGRCAHSIKGAAAGIGGEALRAAAFEAEKAGKAGDANALLKLE
                     PEVREQYELLEAALRQLISSRGGKNE"
BASE COUNT          633 a         1072 c         1168 g          681 t
ORIGIN      
        1 gcgcgaccgc cctcctctca gggaacggat ccgcgccata acgcgcctcc gccctctcga
       61 aggagactga acggctccgt cttaaaaagt agcgaacggt cgggcggaga atagagtata
      121 cttagaggaa tcccgtgaaa ctctagcggg gcctacggcc ccgttgccgc gggacatgaa
      181 gagccggaat cccggaggcg cctatgaaga agaccttttt ccgttcatcg cataaacgcg
      241 ccgcgagtct cgccgatatt cttgtcgtcg tgctcttgct ggcggctgga tttttcctcg
      301 gcgagtggcg cgtgtcgaag gcagagctgg ggatgcgcca taacctggtc cgccgagcgc
      361 gccttttcgc cgaaagcgtc aatcctcgcg cggtcaagcg cctctcaggt tccgaatcgg
      421 accgcgtttc tccgaactac cgccgcttca aagaacagag cgtttcgctc ctctccgcct
      481 ttcccgactg ccgtttcatc tcgctccttg gccgccgtgc cgacgggtcg atcttcattt
      541 acttcgattc cgagcccgaa ggctcgccgg acgagtcgcc ccccggagag aggtatgttc
      601 ctccggatcc cgtactgctg gaggccttcg acaaggggaa gagcgttgtc gccggtccct
      661 ttatggatat ttggggaagc ctcgtcgcgg cgtgggttcc cgtcgccgac ccggataccg
      721 ggaagcccgc gttcgtcgcc gcgctcgata tcgacggcgg gaagtggcgg agcgacgccc
      781 gacgcgccgc atacgttccg gaatgcgcgg cggtccttct ggccgctctc tttatcgtat
      841 ggcggatact gacgctgcgc cgcgccctga ggaggagaac ttcctaccgt tggtatcacc
      901 gctacgccga cgctctcttc gtcgccgcga ccgggctcgt gctgacattt tcgatacttc
      961 tcgtctcatc ggaggttgag cgctcgacgc gtgaggatac ctttcacatg cttgccgact
     1021 cggagtcggg gaagctgcac aacagcctga agagcatcag ggatatcggc gtcgaggggg
     1081 cggcggcgtt cttctcgtac agcgatttcg tcgacttcga ggaattcaag gggtacgccc
     1141 gtcatctgga ggagttgccc gaggtgcggg gcgcgacgtg gcttccgctc gttccgcatg
     1201 aagagcgcga ggcgttcgaa cgtctcggac gctcgctcgt tacagaggat ttcgtcatcc
     1261 gcgattacgg ggagggggga aggaggggcc ccgctcctga gcgtcccttt tattatccgg
     1321 tgttctaccg catacccttt gaggggagcg agcgcatact gggggctgac tttgctcagg
     1381 ttccctccag aagagccgcc atcgacgagg cgcgcgcttc ccgccttccc gcggcgacgg
     1441 aggcgatccc tctgctcgcc gccccgggaa cgctgaaagg gatcgtcgtc tttcagccgg
     1501 tattttcacg caacgaaacg gagaatttca tgggcgtgct cgccatagtc gtcgagttga
     1561 acgctctcct caaccggatg cgcaccgaag aggcgcatgt ggagcgcgcc gtgctcgccg
     1621 gtgatttgtg gcgcctgtat ggcgcgggag ttcacgaaca cgtcgtctcc acgggggaag
     1681 gcgccatttt gccgcctgca tggccgtccg tcggggatct cttctcggtg cgcccccttc
     1741 tcttcttcgg caggacgttc gccgtcattc tgcgtcccgg acccggcttt cgtgcgctct
     1801 atccgccccg tgccggttgg atgagccttt tcgccggcat catggtgacc gctgtgctga
     1861 ccatgctcgc ggttacagtc gcgaaccgca gggaggagct tgagcgccag gtcgcgatga
     1921 gaacggatga gctgcgccga agcaaggacg aactcttcct gatgaacgcg gccctcgtgg
     1981 atacgacgcg caagacgcag gagatagcgc gccgcgccga ggcggccagc gaggcgaagg
     2041 ggcgcttcct cgcgaacatg agccatgaga tgcggacgcc gttgaacggc ttcctcggga
     2101 tgaaccggct gctgctcgac acccgtctga cggaggagca gcgggagtac gccgttgcgg
     2161 ctcgaaagtc cggcgaggcg ctgctgacgc tgataagcga cgcgcttgat attgcgacca
     2221 tcgacgaagg ccgctaccgt ctcgtcgagg aagatttcga cttggacgaa ctgctttcga
     2281 aactggaaac gatcttcagc gcggcagcgc acgagaaagg acttcgtttc acctccgtcc
     2341 gtccgtctgt tccgctgcgg ctgcacggcg acggtcgctg tctttacaga atactgagaa
     2401 gcctcctgga caacgcggtg aagttcaccg tccgcgggga ggtgcgtttt tccgtctccg
     2461 cccttgaaga gagcgatcgg accgttcgcc tgcgcttttc ggtggaggac acgggcgtcg
     2521 gtatcccgtc ggagcagcag gggtgcgtct tcgaacgctt ctggcaaggc gacgactcga
     2581 ccacgaagtc gtaccagggc gcggggcttg gcctccccat ggcgaaggag cttgccgcgc
     2641 ttctcggcgg ggagcttgaa atgcaaagcg aggaggggag gggaacgacg ttttctttcg
     2701 agtcgctctt ccacaagggg gcttcccctg cggcgccttc catacaggac gaatccgtga
     2761 caggcagaag aatactgctt gcggaggaca accctgtcaa ccggaagttt gcgctggcga
     2821 tgcttcgcaa gctggggtac tcggtcgcct ccgctgaaaa cggcaaagaa gccgtggact
     2881 acctctcctc gaacgaatgc gacctggtgg tgatggacgt gcagatgccc gtcatggacg
     2941 gggtggaggc cacgcggatg atccggagcg gcgcgtcggg cgcgctcgac ccgtcgatac
     3001 ccgtcgtcgc gctcactgcc tatgtctctc ctgaggacag ggagcgctgc atggtcgccg
     3061 gaatgaacgc gtttcttccg aagccggcgg ctccggaaga actggatgcc gcaatacgcc
     3121 ggctgcttta cggagaagcg ggaggagagc gcgccgaaac tcacgtgccg gccgaatacg
     3181 ccgggaaagc agcgcccgtc ttcgatcgcg cagccgctct gaaacgcctt atgggcgacg
     3241 aggacgcgct ggcggagctt gcggaggagt tcatcgagga gatgccgaag gatttggaga
     3301 aattttccgc tctggtctcc gaaggggacg ccgccgctgc gggaaggtgc gcgcactcga
     3361 taaagggcgc cgccgccggt atcggcggag aggcgctcag agccgcggcg ttcgaggccg
     3421 agaaagccgg aaaggccggg gacgctaacg cgctcctgaa attggagccg gaagtgcgcg
     3481 agcagtacga gcttcttgag gcggcgctcc gtcaactgat ctccagccgg ggaggaaaga
     3541 acgaatgaac gagc
//
LOCUS       DAOSWW010000169         3538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18766_length_3538_cov_3.003732, whole genome shotgun sequence.
ACCESSION   DAOSWW010000169 DAOSWW010000000
VERSION     DAOSWW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18766_length_3538_cov_3.003732"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PK442_04670"
     CDS             <1..865
                     /locus_tag="PK442_04670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOO86972.1"
                     /translation="EDRDPWPAVREALRQLGVRRLAVEDDKLSLERGRALENLLPSLI
                     GTTRLVQQLRAVKDAEELELLRRSARIAAEAFEAFLPAIRPGRSEAELAADLVHEIRR
                     RGAQQMAKGHFVVASGPRGARPHGIFSDRVVQPGDFITMDFGAVYNGYVSDMTRTVGV
                     GDVDPKLVEIYHIVREAHDRAIAAVSPDATGSDVDEAARSLIRGRGYGEFFSHSTGHG
                     IGLELHELPVVNRMNGEKLPVGSVVTVEPGIYIDGLGGVRIEDDVIVTPHGAEVITSA
                     SGTELRLLSPA"
     gene            972..2480
                     /locus_tag="PK442_04675"
     CDS             972..2480
                     /locus_tag="PK442_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO86973.1"
                     /translation="MKKFRLIALALLAVAALALPAAAAELRIAQVADISSLDPHKVSD
                     IYSANVIRQIYSNLVQVDEHMEIVPDLAESWENPDNLTWIFHLRKGVTFHNGDPLTAA
                     AVKFSLERIKDPATASPGATHLREVASVEAIDEHTLKIVTKRPFAPMLMSLTRYETSI
                     VSERAVKEAGADYGSKAAVGTGPFKFMSWSRGDKVTLEKFDGYFRGPASLDTVVYRGI
                     PEDATRLVELESGGVELIPGNFPYQDFVTIEESGKFLTYQTPAMSTLYMGLNTEIKPF
                     DNKLVRRAMNYAVDKQAIVDAIYFGFGVPAKGPLSPVIWGFDPTRGPAYPYNPDKAKE
                     LLKEAGFPDGFDMTIFSDNRTERRSAAELIQAYLSEVGIRAQVELMEWSALLSTSAKG
                     IGGAFMLGWTGTGDADGGMTPVYHTANIGSSNRFRYSIPALDALLEKGAATLDQTERR
                     KIYAEAQQLAVDECPLVFMISQELLGASSKKVQGFKLYPNMISPLYGVTVAK"
     gene            2556..3491
                     /locus_tag="PK442_04680"
     CDS             2556..3491
                     /locus_tag="PK442_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO86974.1"
                     /translation="MLRYFFKRMMFLIPVLFGVSILIFSIVHLAPGDPAEVMLGPAAT
                     QEDIANLREQLGLTKPLYVQYGIFLGNALKGDLGRSIKSNAPVMEEIMERFPATLTLA
                     LLGMTFAIVIGLPLGILAALKQNTWVDALCSFLALVGFSIPNFWIALMLMLLLSIYIP
                     ILPSSGFDSWKNLIMPTIVLGIQTMAVIARMTRSSMLEVIRQDYVRTAKAKGISDRLV
                     LTRHILSNVMIPVVTIAGLYFGHQLGGVVITETVFSIPGLGRLIVDAIRAQDYPVVQG
                     GVLFFAFCVGIVNLLVDLLYAVLDPRIKAQYRAAK"
BASE COUNT          676 a         1194 c         1073 g          595 t
ORIGIN      
        1 ggaggaccgc gacccgtggc ccgccgtgcg ggaggcgctc cggcagctgg gcgtgcggcg
       61 gctcgcggtg gaggacgaca agctcagcct cgaacggggg cgcgcgcttg aaaacctgct
      121 cccctcgctg atcggcacaa cgcgccttgt acagcagctc cgcgccgtca aggacgccga
      181 ggagctggag ctactgcggc gctcggcccg gattgcggcg gaggccttcg aggccttcct
      241 tccggcgata cgccccggca gaagcgaggc ggagctcgcc gccgacctcg tccacgaaat
      301 ccgccgcagg ggagcgcagc aaatggcgaa ggggcacttc gtcgtggcct ccggacctag
      361 gggcgcgcgt ccgcacggca tcttctccga cagagtcgta cagccgggcg acttcatcac
      421 aatggacttc ggcgcggtgt acaacggcta cgtctcggac atgacccgca ccgtgggcgt
      481 cggcgacgtc gacccgaaac tcgtcgagat ctaccacatc gtccgcgagg cgcacgaccg
      541 cgcgatcgcc gcggtttctc ccgacgcgac gggaagcgac gtcgacgagg cggcgcggag
      601 cctcatccgc ggccgcgggt acggcgagtt cttttcgcat tcgacggggc acggcatcgg
      661 gctggagctg cacgagctgc cagtggtcaa ccgcatgaac ggcgaaaagc tccccgtcgg
      721 ctccgtggtg accgtcgaac cgggcatcta catcgatggc ctgggcggcg tgcgcatcga
      781 ggacgacgtg atcgtcacgc cgcacggcgc ggaggtgatc acaagcgcct ccgggacgga
      841 gctgcgtctg ctctccccgg cgtagaaaaa agtaccgggg ttctccccgg tgaagcatag
      901 atagattgcg cacccactcg aagacgccgg aaaaagcggc gtcgaaaaaa acagaaaggt
      961 cgtgatgcac agtgaaaaag ttccgtctca tcgcactcgc gcttctcgcc gtcgcagcgc
     1021 tcgctcttcc cgccgccgca gccgagctgc ggatagccca ggtcgccgac atctccagcc
     1081 tcgacccgca caaggtcagc gatatctact ccgcgaacgt gatccggcag atctacagca
     1141 atctcgtgca ggtcgacgaa cacatggaga tcgtccccga cctcgccgag agctgggaga
     1201 accccgacaa cctcacgtgg atcttccacc tccgcaaggg cgtgaccttc cataacggcg
     1261 atccgctcac cgccgccgca gtgaagttct ccctcgaacg gatcaaggac ccggcgaccg
     1321 catcccccgg cgccacccat ctgcgcgaag tggcctccgt cgaggcgatc gacgagcaca
     1381 cgctgaagat cgtcacgaag cggccgttcg cccccatgct gatgagcctg acccgctacg
     1441 agacctccat cgtaagcgag cgcgccgtca aggaagcggg cgcggactac ggcagcaaag
     1501 ccgccgtggg caccggtccc tttaagttca tgagctggtc tcgcggcgac aaggtcacgc
     1561 tcgaaaagtt cgacggctac ttccgcggcc ccgcgtccct cgatacggtg gtctacagag
     1621 gtattcccga ggatgcgacg cgcctcgtcg aacttgagtc cggcggcgtc gaactcattc
     1681 ccggcaactt cccctaccag gacttcgtaa caatcgagga gagcggcaag ttcctcacct
     1741 accagacgcc cgcgatgtcc acgctctaca tggggctgaa caccgagatc aaacccttcg
     1801 acaacaagct cgtccgccgg gcgatgaact acgcggtcga caaacaggcc atcgtcgacg
     1861 ccatctactt cggcttcggc gtcccggcga agggacctct ttcgcccgtc atctggggct
     1921 tcgacccgac gcgcgggccg gcctacccct acaatccaga caaggccaaa gagctgctga
     1981 aagaggccgg attccccgac ggattcgaca tgaccatctt ctccgataac cgtacggagc
     2041 gccgcagcgc cgccgagctg atccaggcgt acctctccga ggtcggcatc agagcgcagg
     2101 tcgagctgat ggagtggagc gcgctgctct ccacctccgc aaagggtatc ggcggcgcgt
     2161 tcatgctcgg ctggacaggc acgggcgacg ccgacggcgg catgaccccc gtctatcaca
     2221 ccgcgaacat cggctcctcc aaccgcttcc gctactccat ccccgcgctc gacgcgctgc
     2281 tggagaaggg cgcggccacg ctcgaccaga ccgagcgcag aaagatctac gcggaggcgc
     2341 agcagctggc cgtcgacgag tgtccgctcg tcttcatgat cagccaggag cttctgggcg
     2401 cgtcctcgaa gaaggtgcag ggattcaagc tctatccgaa catgatctcc ccgttgtacg
     2461 gagtgaccgt cgcgaaataa ggcggacgaa cgcgccggag ttcccgcctc gcggcggcag
     2521 aactccggcg atatttcccc tggaggactg gttgcatgct tcgatacttt ttcaagagaa
     2581 tgatgtttct catccccgtg ctcttcggcg tctccattct catcttctcc atcgtccacc
     2641 tcgcccccgg cgaccccgcc gaagtcatgc tcggccccgc agcgacgcag gaggacatcg
     2701 ccaacctgcg ggagcagctc ggcctgacga agcctttata cgtccagtac ggcatctttc
     2761 tgggcaacgc gctcaagggc gacctgggcc gctcgatcaa gtcgaacgcc ccggtcatgg
     2821 aagaaatcat ggagcgattc ccggcgacgc tcaccctggc gctgctggga atgacgttcg
     2881 ccatcgtcat agggctgccg ctgggcatcc ttgcggcgct caagcagaac acgtgggtgg
     2941 acgccttatg cagcttcctg gccctcgtcg gtttttcgat accgaacttc tggatcgcgc
     3001 tgatgctcat gctgctgctc tccatctaca tcccgatcct tccgtcgtcc gggttcgact
     3061 cgtggaagaa cctcatcatg ccgaccatcg tgctcggcat ccagacaatg gccgtcatcg
     3121 cgagaatgac ccggtcgagc atgctcgaag tcatccggca ggactacgtc cgcacggcga
     3181 aggcgaaggg catatccgac cggctcgtac tcacgcggca catcctgagc aacgtgatga
     3241 tcccggtggt cacgatcgcg gggctctact tcggtcacca gctcggcgga gtggtcatca
     3301 ccgagaccgt cttctccatc cccgggctgg ggaggctcat cgtcgacgcc atcagggcgc
     3361 aggactaccc ggtggtgcag ggtggcgtgc tcttcttcgc cttctgcgtc ggcatagtga
     3421 atctgctcgt ggacctgctg tacgccgtgc tcgatccccg gatcaaggcg cagtacaggg
     3481 ccgcgaagtg acggagagga gcgaacactc atgaacagtg aaaggcaatc gatcgtca
//
LOCUS       DAOSWW010000170         3537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18775_length_3537_cov_4.009477, whole genome shotgun sequence.
ACCESSION   DAOSWW010000170 DAOSWW010000000
VERSION     DAOSWW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18775_length_3537_cov_4.009477"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..639)
                     /locus_tag="PK442_04685"
     CDS             complement(70..639)
                     /locus_tag="PK442_04685"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOO86975.1"
                     /translation="MEKEKLFHRMLDVIEGDIVPLTARGVSIGSKVFGAAVLRRDDLS
                     LVVAGTNHEAFSPLWHGEVYTIKLFYELQGRPDPSECLFLSTHEPCSMCISALSWAGF
                     KDIYYFFGYEQTQDDFNIPHDLRMLREVFGCATPTKENIYYTSRSLMDIAEGLEDLAA
                     ARARIARIKETYAGLSAVYQAGEKKMVLK"
     gene            complement(800..1129)
                     /locus_tag="PK442_04690"
     CDS             complement(800..1129)
                     /locus_tag="PK442_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86976.1"
                     /translation="MRKIVLIAVLWGAMFGSAFAEPAPAAWKGDLRHVVERGGVSYSI
                     YADRTRLVEDCVPGAEQVLETFVHLVIESQNLVEIQQWNIRQDGSGYRVQDMYDYTLD
                     TFGMVDQ"
     gene            1254..1682
                     /locus_tag="PK442_04695"
     CDS             1254..1682
                     /locus_tag="PK442_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO86977.1"
                     /translation="MTVKYLRVVNRDQCIGCFSCMYACSRMVKNAAGSGKAALRVKAY
                     TGVEGAFSIRVCARCEEPDCAAACPTGALTKAPGGGVRLKKELCISCMACVKACAIGA
                     LQWDEEEKRPLPCIHCGQCVKYCPNNVLALCEKEEECDPR"
     gene            1679..3412
                     /locus_tag="PK442_04700"
     CDS             1679..3412
                     /locus_tag="PK442_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOO86978.1"
                     /translation="MNGAILHVDMTTGTHRMIDDPELFGEFLGGTAVATELLFRHGDP
                     KGDPLAPESPVIFAIGPFSSLFPVATKTVAMFKSPLSGELGESHAGGRMAMSLHEAGI
                     KALVITGKADTLSYLVIENDSAELKSASTFRGQSAAATERILREAEGKSGRKVSILRI
                     GPAGERLSPMACATVDGSRHFGRLGLGAVLGSKNLKAIVISGTRTLPLARPKEFKAVY
                     DILYDRVVKSKEMKKYHDLGTPANVRPLSLIRGLPTRNFSQGNFEGAEAISGERFAKD
                     FLVQHTACAHCQCGCIHMAELREEFAPYHYSTNRVSYDYELIYAMGSMLSLSSPEDIL
                     KLLLFTEKQGWDAISLGCTLAWATESFLHGVLSLDDTGGVSLSFGDCGAYLEVLERMA
                     KGESEFYRDLEMGCAFCAKKRGGLEWAIQYGGVEPGGYMTGENFAVTCLLGVRHSHLD
                     DNGYSIDQNLLNAPKPLEEQVRAQVKEARWRMVLNSLVICLFARGVYDEEIILQGLDA
                     LGAQWDAGKLRELAAKALLRKHEWKRLCGFDPAALVVPEKMYTVQTSTGMIDKESLQQ
                     RIHLYLEEIRR"
BASE COUNT          725 a         1146 c         1029 g          637 t
ORIGIN      
        1 tcctcgcggc gcagggggat ttttcgttca tcggattgta ccgccgacgc gcatcttgca
       61 catcccgcgc tacttaagca ccatcttctt ctcgccagcc tggtagacgg cggacaaccc
      121 ggcgtacgtc tctttgatcc tggcgattct cgccctggcc gccgccagat cctccaaccc
      181 ctccgcgata tccatgagcg aacggctggt atagtagata ttctccttcg tcggcgttgc
      241 gcagccgaag acctcgcgca gcatccgcag gtcgtgcggg atgttgaagt cgtcctgcgt
      301 ctgctcgtag ccgaagaagt agtagatgtc tttgaatcct gcccacgaca gcgccgaaat
      361 gcacatcgag cacggctcgt gcgtcgagag gaagaggcac tccgaagggt ccgggcgccc
      421 ctgcagctcg tagaagagct tgatggtgta cacctcgccg tgccacagcg gggagaaggc
      481 ttcgtggttc gttcccgcaa ccaccagcga gagatcgtcc ctccgcagca ccgccgcgcc
      541 gaacaccttg ctcccgatgc tcaccccccg cgccgtcagc ggaacgatat ccccctcgat
      601 gacgtcgagc atacgatgaa acagcttctc tttctccaca aaaacagctc ccttcaaaga
      661 gtatcggaac gcatgaacgg attccggcgc agagtcaatc gcctcgctcc gctacaatca
      721 tctgtgcagc ccggataaca tcccgctgat acgagtcctc ctccacgggc ctccacgagg
      781 cccactcggg caggacgatt cactgatcca ccatgccgaa cgtatccagc gtatagtcgt
      841 acatatcctg aacgcggtac ccgcttccat cctgccgtat gttccactgc tggatctcga
      901 ccaggttctg cgattcgatc accagatgca caaacgtttc gagcacctgc tccgcgcctg
      961 gaacacagtc ctccaccaga cgggttctgt ccgcatatat ggaataggag accccgccgc
     1021 gctccacaac atgccgcaga tctcccttcc acgccgcagg agccggctcc gcaaacgcgc
     1081 ttccaaacat cgcgccccaa aggacggcga tcaacacaat tttcctcatc gctcccacct
     1141 ccgtttattc gaaagggctc gaattctttt ctctttgttc attgtagcag ttgacaggga
     1201 gaaaaagaca ggggattatt gcggagagag aaaaaaagtg agaggtggaa cgcatgactg
     1261 tcaaatattt acgagttgtc aaccgtgacc agtgcatcgg ctgcttcagc tgcatgtacg
     1321 cgtgcagccg catggtcaag aacgcagccg gcagcggcaa agcggccctg cgggtcaagg
     1381 cctataccgg cgtggaaggc gcgttctcaa tccgcgtctg cgcacggtgc gaggagccag
     1441 actgcgccgc ggcatgtccg acgggagcgc tgacaaaggc cccgggcggc ggagtgcgcc
     1501 tgaaaaaaga gctctgcatc tcctgcatgg cgtgcgtcaa ggcgtgcgcg atcggcgcgc
     1561 tccagtggga cgaggaggag aagcgccccc tcccctgcat ccactgcggc cagtgcgtta
     1621 aatactgccc caacaacgtc ctcgcgctct gcgagaaaga agaggagtgt gatccgcgat
     1681 gaacggggct attcttcacg tcgacatgac gacgggaacg caccgcatga tcgacgatcc
     1741 cgaactcttc ggcgaatttc tgggaggaac cgccgtcgcc acggaactgc tcttccgcca
     1801 cggagatccg aagggcgacc cgctcgctcc cgagagcccg gtcatcttcg cgatcggtcc
     1861 gttcagctcg ctctttcctg tggcgacgaa gaccgtcgcg atgttcaaat cccccctctc
     1921 cggcgaactc ggggagtcgc acgcaggcgg ccgcatggcg atgtcgctgc acgaagcggg
     1981 aatcaaggcg ctcgtgatca ccggcaaagc ggacacactc tcctacctcg tcattgagaa
     2041 cgactccgcc gaactcaagt ccgcctccac cttcaggggg cagtccgccg ccgccaccga
     2101 gcgtattctg agagaggctg aggggaagag cggacgcaag gtctccatcc tccgcatcgg
     2161 ccccgccggc gaacggctct cacccatggc gtgcgccacg gtggacggtt cgcggcactt
     2221 cggcaggctg ggacttggcg cggtcctcgg cagcaagaac ctcaaggcga tcgtcatcag
     2281 cggaaccaga accttgccgc tcgcccgccc gaaggagttc aaggccgtct acgatattct
     2341 ctacgaccgg gtggtcaagt cgaaggagat gaagaagtac cacgatctcg gcaccccggc
     2401 caacgtccgg ccgctctccc tcatcagggg gcttccgacg cgcaacttct cgcaggggaa
     2461 cttcgagggg gccgaggcga tctccgggga gcgcttcgcg aaggacttcc tcgtccagca
     2521 caccgcctgc gcccactgcc agtgcggctg catccacatg gccgagctgc gcgaggagtt
     2581 cgccccctac cactacagca caaaccgcgt gtcctacgac tacgagctga tctacgcgat
     2641 ggggagcatg ctcagcctct cctcccccga ggatatcctc aagctgctgc tcttcaccga
     2701 gaagcagggg tgggatgcga tatcgctcgg ctgcaccctc gcctgggcca cggaatcctt
     2761 cctccacggc gtcctctcgc tcgacgacac cgggggagtt tcgctctcct tcggcgactg
     2821 cggagcgtat ctcgaagtcc tcgaacgcat ggcgaaagga gagagcgagt tctaccgcga
     2881 ccttgaaatg gggtgcgcgt tctgcgcgaa gaagcgcggc gggcttgaat gggccatcca
     2941 atacggcggc gtggagcccg gcggctacat gacgggcgag aactttgcgg tcacctgcct
     3001 cctcggcgtg cgccactccc atctcgacga caacggttac tccatcgacc agaatctgct
     3061 gaacgcgccc aaaccattgg aggagcaggt ccgcgcgcag gtgaaggagg cgcgctggcg
     3121 catggtgctc aactcgctgg tgatctgcct cttcgcgcgc ggcgtctatg atgaggagat
     3181 tatccttcaa gggctcgacg ccctcggcgc gcagtgggac gccgggaagc tcagggagct
     3241 ggcggcgaaa gcgctgctgc ggaagcacga gtggaaacgg ctgtgcggat tcgatcccgc
     3301 cgcgctcgtc gtgcccgaga agatgtacac ggtacagacc agcaccggaa tgatcgacaa
     3361 agaaagcctg cagcaacgta ttcatctcta tcttgaagaa ataaggaggt aagcgcagag
     3421 gctatcgtcg acctgagcac gcgggaagac gaaggcgcca gcctctctca actggcaaag
     3481 gccgatccgg agcgggtgat catcctcagg tcggtttcca acttcgccat gcagtgg
//
LOCUS       DAOSWW010000171         3536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18782_length_3536_cov_4.131284, whole genome shotgun sequence.
ACCESSION   DAOSWW010000171 DAOSWW010000000
VERSION     DAOSWW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18782_length_3536_cov_4.131284"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK442_04705"
     CDS             <1..655
                     /locus_tag="PK442_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO86979.1"
                     /translation="MTGLGQVIAMNIIMFAGGKLWAALILCMIAAIILGMGLPATPCY
                     IITATIAAPALQQMGVPPLAAHFFAFYYGTMSAVVPPVALTSYTAAGLAGARPFKVAM
                     SALGLAVSGLLIPYLFVYNPDLLFVDFVLTKYVLDLSIAIIGIFSLSCGIIGMLRREM
                     EFWERALFVAAGILMVNPLRVLRISSLGFFALILAFHLFSSRGANAQRSGGETKEEK"
     gene            870..1487
                     /gene="infC"
                     /locus_tag="PK442_04710"
     CDS             870..1487
                     /gene="infC"
                     /locus_tag="PK442_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOO86980.1"
                     /translation="MNREITSREVLIIDENGVKLGTLPTAEGIRMAEERMLDLVEVAP
                     QATPPVCRILDYGKYRYQLQKKEKDARKKQKVQTLKEMKMRPKIDEHDYDFKVRAIKS
                     FLEDGHRVKVSVFFRGREMSFLDKGEAVLARVMRDCDGLGKSEGNPRMEGRYMRIMLT
                     PVPQAQIKPTPKPAAKNDVPKAEKKPDETKVNPAPEGAESRQVSE"
     gene            1590..1787
                     /gene="rpmI"
                     /locus_tag="PK442_04715"
     CDS             1590..1787
                     /gene="rpmI"
                     /locus_tag="PK442_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOO86981.1"
                     /translation="MPKLKSHSGAKKRFSFTGTGKLSYRRVGRGHHLTVKGPARLRKL
                     RKTGYVDATRIESLKKLLPYN"
     gene            1822..2193
                     /gene="rplT"
                     /locus_tag="PK442_04720"
     CDS             1822..2193
                     /gene="rplT"
                     /locus_tag="PK442_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOO86982.1"
                     /translation="MPRVKGGSASDKKRKKLFSITKGYWGRKKNVYRRAREAYLHSLS
                     RAYVDRKRKKRDFRRLWITRINAATRSCGMSYSAFMNGLKKSGILINRKMLSELAIND
                     MAAFPVDGSSKERTGQVDQRL"
     gene            2524..>3536
                     /gene="pheS"
                     /locus_tag="PK442_04725"
     CDS             2524..>3536
                     /gene="pheS"
                     /locus_tag="PK442_04725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOO86983.1"
                     /translation="MQTELESIEQRFTEQLSGIASSEELQQVKVAFLGKKGAVTSFLK
                     TLGSLSPEERPVAGQAVNSLRDRMEAILDEKKSFLEEKESDAAERAYRIDVTLPPRGR
                     SIGGIHPVAQLTQDVVEILAGLGFSVALGPEIEDDFHNFEALNIPASHPARDMQDTFY
                     FPDGKLLRTHTSPVQVRSMLRYGAPLRIVCPGKVYRRDSDPTHSPMFNQLEGLLVEKD
                     ISVADMKGCLETLMAAIFSRPLKARYRASYFPFTEPSLELDIECVECSGKNPSCRICK
                     GTGWLEVAGLGMVHPNVIRSGGIDPDVYNGFAWGIGLDRIAMLKYGLTDLRVLFDGNV
                     PYLL"
BASE COUNT          841 a          952 c         1007 g          736 t
ORIGIN      
        1 aatgacgggc ttggggcagg tcattgcgat gaacatcatc atgttcgcag gcggcaagct
       61 ctgggcggcg ctcatcctct gcatgatagc ggccatcatc ctcggcatgg ggctgcctgc
      121 gacgccatgc tacatcatca ccgccaccat tgccgccccc gccctgcagc agatgggagt
      181 ccccccgctt gccgcgcact tcttcgcgtt ctattacggt accatgtccg cggtggtacc
      241 gcctgtcgcc ctgacaagct acacagccgc cggtcttgcc ggagcgcgtc cgttcaaagt
      301 tgcgatgtcc gcccttgggc tggccgtttc cgggttgttg attccgtacc tctttgtcta
      361 caatccggat cttctgttcg tcgacttcgt tctgacgaaa tacgttctgg atctgtccat
      421 agcgatcatc ggcattttct cgctctcgtg cggaatcatc ggaatgctgc ggcgggagat
      481 ggaattctgg gagcgagcgc tcttcgtcgc cgcgggaatc ctgatggtca atccgctgag
      541 agtcctcagg atatcaagtc tcggattctt tgcgctcatt cttgcctttc acctcttttc
      601 aagcagagga gcgaatgcgc agagaagcgg gggagagacg aaggaagaaa aatagaattt
      661 gcgtacttcg ggtgttcgtg ctataatgcc ttagcttaaa aatattgaga agaagagggg
      721 ccaccctctc accttgcgga gcgctatttc gcctggtttg aagagaagtc ttcgcgtatg
      781 cggggccggt ggttttccat cggccccttt tatatttcta ccggaggtga aaggctatag
      841 taacacgaaa agacgatgga gaaccccgag tgaaccggga aattacctca cgagaagtcc
      901 tgattatcga tgagaacggc gtgaaactgg gcaccctgcc cacggccgag ggaattcgga
      961 tggccgagga gagaatgctc gaccttgttg aggtggcgcc gcaggcaacg cctcccgtat
     1021 gcagaattct cgattacggt aaataccggt accagttaca gaaaaaagag aaggacgccc
     1081 ggaagaagca gaaagtgcaa accctgaagg aaatgaagat gcggcccaag atcgacgagc
     1141 acgactacga cttcaaggtc agggccatca aaagtttcct tgaggacgga cacagagtca
     1201 aggtctccgt cttcttcagg ggaagagaga tgtccttcct tgacaaggga gaggcagttc
     1261 tcgcccgggt aatgcgagat tgcgacggac tcggcaagag cgagggaaat ccccgcatgg
     1321 aggggcggta tatgcggatc atgctgactc ctgtacctca ggcccagata aagccgacgc
     1381 ccaagcccgc tgcgaaaaac gatgttccca aggctgaaaa gaagccggac gaaacaaaag
     1441 tgaaccccgc ccccgaggga gcggaaagcc ggcaggtttc agaataagtc cgaacaggac
     1501 gcttggaatg cgatgacgtt caatccgcct gtcgcgaact gcggcgggct tttatcttga
     1561 tgaatatccg aggaaggagg aatgctcaaa tgccaaagct taaatcccat tcgggagcca
     1621 aaaaaaggtt ttcgttcaca ggaacgggga agctctccta ccggagagtg ggaagaggcc
     1681 atcatcttac ggtgaagggt cccgctcgcc ttcgcaagct gcgcaaaaca gggtatgtcg
     1741 acgcaacccg tatcgagagt ctgaagaagc ttctcccgta caactgagaa caagtttatt
     1801 ctatgaaaga ggtgacgtgc gatgccccgc gtcaaaggtg gtagcgcaag cgataaaaag
     1861 agaaaaaaac ttttttcgat aaccaaagga tactggggac ggaaaaagaa tgtgtatagg
     1921 agagcgagag aggcgtacct tcactccctt tccagggcgt acgttgacag aaaacgcaag
     1981 aagagagatt tccgccgcct gtggatcaca agaatcaacg ctgcaaccag atcctgcgga
     2041 atgtcctaca gcgccttcat gaacggcctg aaaaaatcgg gaatcctcat caacaggaag
     2101 atgctttccg agctggcgat caacgacatg gctgcgttcc cagttgacgg ttcaagcaaa
     2161 gaacgcactg ggcaagtaga ccagcgcctt taaaagcgcg agacaggttg tgcggcgcac
     2221 tacacgcaac cgcgcagcct cctaaaaggc catttcgagg gtttctgccg ccacgcagcg
     2281 gcgggaaccc tttttctttg cctgtggagc ctcggcggat gtggtgatat aatccactct
     2341 gaagtgaaat tttcgaacga agtataatgt ttctgtcctt cttttctacc aaagacgctg
     2401 cgctctcccc gtcccgatcc gatcgtccat gcgggagatt tgccgttgca ggcctgtgtg
     2461 gaggaggata caatcgcttt gaccggaata ttaggagaag gaggagaacg gcacgtgaca
     2521 tccattcaga ctgaactgga aagcattgaa caacgtttta cggaacaact atcgggaatc
     2581 gcttcttccg aggaacttca gcaggtgaag gtcgcttttc tcggcaagaa gggcgccgtg
     2641 acctcctttt tgaaaacgct tggttccctt tctccggagg aacgcccggt tgccgggcag
     2701 gcggtgaact ccctccgcga cagaatggaa gcaattttag acgaaaagaa gagcttcctc
     2761 gaggagaagg aatccgacgc cgcggagcgg gcataccgca tcgacgtcac tctgcccccg
     2821 agggggagga gcatcggcgg aattcacccg gtcgcgcagc tgacgcaaga tgtggtggag
     2881 attctcgcgg ggcttggttt ttccgtagcc ctcggtcccg agatagagga cgactttcac
     2941 aacttcgagg cgctcaacat cccggcgtcc caccctgcaa gggatatgca ggacacgttc
     3001 tatttcccgg acggaaaact gctgcggaca catacgtcgc ctgtgcaggt acggtcgatg
     3061 ctccgctacg gagctccgtt gcgaattgtc tgccccggca aggtgtaccg gcgcgacagc
     3121 gaccccacgc actcgcccat gttcaaccag ctcgaaggcc tgctcgtaga gaaggacatc
     3181 tccgtggccg acatgaaggg gtgccttgaa acgctcatgg cggcgatctt ttcccgcccc
     3241 ctcaaagcgc gctaccgggc gagctacttc ccctttaccg agccgtcgct cgaactcgac
     3301 atcgagtgcg tcgagtgttc cggcaagaac ccctcgtgca gaatctgcaa agggactggg
     3361 tggctcgaag tcgcgggtct cggcatggtg cacccgaacg ttatccgttc cggaggcatc
     3421 gatccggatg tgtacaacgg ttttgcctgg ggaatcggcc tcgaccgcat cgccatgctc
     3481 aaatacggac tgactgatct gcgcgttctc tttgatggca acgtgcccta tcttct
//
LOCUS       DAOSWW010000172         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_18916_length_3516_cov_4.568911, whole genome shotgun sequence.
ACCESSION   DAOSWW010000172 DAOSWW010000000
VERSION     DAOSWW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18916_length_3516_cov_4.568911"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PK442_04730"
     CDS             complement(<1..712)
                     /locus_tag="PK442_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO86984.1"
                     /translation="MSDEKRKLLAASPLFFTFFSVLTGLLVGALVLLISGYSPLKAYH
                     VIIDGIFSRPSYIVYTVIYSTPLIMTGLSVAFAFRTGLFNIGAEGQFIMGALGAALAG
                     AFLHLPPVLHAIVAALAGIAAAAVWGGIAGFLKARFGVNEVIATIMLNWTALYLNNFI
                     VTLPSIKRTATNATAFILDSARIEILPLWKVSEAGRTWRAMHPFWHDIFRTPFNAGIP
                     VALFLAFFCWWILNRTTLG"
     gene            complement(705..2249)
                     /locus_tag="PK442_04735"
     CDS             complement(705..2249)
                     /locus_tag="PK442_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO86985.1"
                     /translation="MPERKKNAAELAVEMKQITKRFGNFVANDSIDFDVRRGEVHALL
                     GENGAGKSTLMNQLYGLYLPSSGEIFINGKKVRITGPKIAIARGIGMVHQHFMLVQPF
                     TVAENIVLGCESVRAFGILDTTRAFQEVEVLSRRYGLAVDPGARIQDISVAMQQRVEI
                     LKCLYRGAEILILDEPTAVLTPQEIDFLAEILQNLTAEGKTVIIITHKLREIKKMAHR
                     CTVIRRGVKIGTVDVASRSESELAEMMVGRAVSFDVEKTPARVQEPVLLLTDIYALNN
                     RGLPALKGISLVVKRGEILALAGIDGNGQSELVEVLTGLRPVESGSIRFRNTEITDFP
                     SGKRIDMGLAAIPEDRQKRGLIGPFTIAENLILEERSLPPFSRSGILDLQAISRHAKT
                     LIDRFDIRPPREQYLATSLSGGNQQKVILAREITRDPELLIASQPTRGLDVGAIEYVR
                     KRLVQVRDAGKAVLLISLDLDETLALADRIAVIYEGRIVAVLDREETDERTLGLLMAG
                     GGAVHV"
     gene            complement(2318..3313)
                     /locus_tag="PK442_04740"
     CDS             complement(2318..3313)
                     /locus_tag="PK442_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO86986.1"
                     /translation="MKALRVLFALIVLLVFALSSGASQLSIAMITDVGGVNDQSFNQS
                     AWEGLQRAEKDLGVKAAYIESRQDADYGPNLETLLDAGTDLIWGIGFKMADAVLEAST
                     NNVDQKYAIIDFAWEKTPENLIGVVFKAEEASFLVGYIAAKMTKTGKVGFIGGMSFPV
                     IWGFEYGFRAGVAQAGKNVEILSQYAESFTDAAKGKSIANSMYQQGADIIFHASGGVG
                     DGLIEAAKEQGKWAIGVDRDQNYLAPDNVLTSAMKRVDNAIFNIAKELIEGKWQGGHT
                     VSYGLAEGGVDIAPTTSKHVPAELLAEVEAVKARIIAGEVVVPFSKETFASFEVK"
BASE COUNT          794 a          924 c          945 g          853 t
ORIGIN      
        1 aaccgagcgt cgtcctgttc aggatccacc agcagaaaaa agcaaggaag agcgcgaccg
       61 gtattccggc attgaagggt gttctgaaaa tgtcgtgcca gaatggatgc atagcgcgcc
      121 aggttcttcc cgcttcgctt actttccaca gcggcagtat ttcgattcgc gccgagtcca
      181 gaatgaatgc cgtcgcattt gttgcagttc gtttgattga cggcagcgta acaataaagt
      241 tgttcaggta gagggctgtc cagttgagca taattgtggc gatgacttcg ttgacgccga
      301 agcgcgcttt aagaaagccc gcgatgcctc cccagacggc agccgctgcg atgcccgcca
      361 gtgcggcgac gatggcgtgg agcacaggag gcaggtgtag aaaagcgcct gcgagtgccg
      421 cgcccaaggc gcccatgatg aactgtccct ccgccccgat attgaaaaga cctgtccgga
      481 aggcgaaagc gactgagagc cccgtcatga tgagcggcgt ggagtaaatg actgtgtaga
      541 cgatatagct cggtctggag aaaatcccat cgatgatcac atgatacgcc ttgagagggc
      601 tgtatccgct gatcagaagc acgagggcgc cgacaagaag ccctgtgagc actgaaaaaa
      661 aagtgaagaa gagaggcgaa gctgcaagta gcttcctttt ttcgtcagac atgcactgct
      721 ccacctcctg ccatgagaag cccgagcgtg cgctcgtctg tctcttcccg gtcaagaacg
      781 gcaacgatgc ggccttcgta gatgactgcg atacggtcgg cgagagcaag cgtttcgtcg
      841 agatcgagag agataaggag aacagctttc cctgcgtcgc gaacctggac gagtcttttg
      901 cgtacgtatt caatggcgcc cacgtcaagt ccccttgtcg gctgcgacgc gataagcagc
      961 tccggatcgc gtgtgatttc ccttgcgagg atgaccttct gctggtttcc tccggagagc
     1021 gacgtagcga gatactgttc gcggggaggg cgaatatcga aacgatcaat gagcgttttg
     1081 gcatgacggc tgatcgcttg cagatcaaga ataccgctgc gcgaaaaagg cgggagtgag
     1141 cgctcctcaa ggataaggtt ctcggcgatc gtgaaggggc cgatgaggcc gcgtttttgg
     1201 cgatcctccg ggattgccgc caagcccatg tcgattctct ttccggatgg gaaatccgtg
     1261 atctcagtgt tgcggaaacg gatggagccg gattcgacgg ggcggaggcc ggtcaagacc
     1321 tctacaagct cgctctgccc gtttccgtcg attcctgcaa gagccagaat ctcacctcgt
     1381 tttactacga gcgatatacc tttgagcgca ggaagtcccc tgttattgag ggcataaata
     1441 tcagtaagca agagcacagg ctcttgcact cttgcaggcg ttttctcgac atcgaaagaa
     1501 acggcgcgtc cgaccatcat ttcggccagc tcgctctcgg agcgggacgc aacgtcgaca
     1561 gtccctatct ttacgcccct cctgataacg gtgcagcgat gcgccatttt ttttatctct
     1621 cgtagtttat gggtgataat gatgactgtt ttcccctcag cagtcagatt ttggagtatc
     1681 tcggcaagaa aatcaatctc ctggggagta agaactgccg tcggttcgtc gagtatgagg
     1741 atttctgccc cacggtagag gcacttcaaa atttccacgc gctgctgcat agccacagaa
     1801 atatcctgaa ttcgtgcgcc aggatcgaca gcaagtccgt aacggcgcga aaggacttcc
     1861 acctcttgga acgcacgtgt ggtatcaagg atgccgaatg ctcgaaccga ttcacagccg
     1921 agaacgatgt tctcggctac ggtaaacggc tgcacaagca tgaaatgctg atgcaccatt
     1981 cctattcccc tggcgatggc tatcttgggg ccggttatcc ggactttttt accattgatg
     2041 aaaatctctc ccgaagaagg aagataaagg ccgtagagct ggttcatcag cgtgcttttt
     2101 cccgcgccgt tttcaccgag gagcgcgtgg acctcgccgc ggcgaacatc gaaatcgatt
     2161 gagtcgtttg cgacgaagtt tccgaatcgc tttgtaattt gtttcatctc taccgccagt
     2221 tcggcagcgt tctttttacg ttccggcaca accccacctc ctcagaatag aaaaggtgcg
     2281 cggaggattt tttgatgtac ttcccccgcg ctcgtttcta tttcacctcg aaggaagcaa
     2341 aggtctcttt gctgaatggg acgacaactt ctccggcgat gattcgcgct ttaacagctt
     2401 ccacttccgc gagcagctcc gctggaacgt gttttgatgt tgtgggggca atatctactc
     2461 cgccctccgc gagtccgtaa gaaaccgtgt gcccgccttg ccatttaccc tcgatcagtt
     2521 cctttgcgat gttgaatatc gcgttgtcaa cgcgtttcat ggcgctggtg agaacattgt
     2581 cgggagcaag atagttttgg tctctgtcaa cgccaatcgc ccacttcccc tgttccttag
     2641 ccgcttcgat cagaccgtcg ccgacgcccc ctgatgcatg gaagatgatg tcggcgccct
     2701 gctggtacat gctgttggcg atggatttgc cctttgcagc gtcagtgaag gattctgcgt
     2761 actggctcag gatttcgacg ttctttccag cttgcgcaac acctgcgcgg aacccgtatt
     2821 cgaatcccca gatcacaggg aatgacatgc cgccaatgaa gccgaccttt cctgtcttcg
     2881 tcatcttcgc cgctatgtaa ccgacgagga aggatgcttc ttcagctttg aagacaacgc
     2941 cgataaggtt ttccggtgtt ttttcccagg cgaagtcgat aatggcgtat ttctgatcga
     3001 cgttgttcgt ggaagcttcc agtactgcgt cggccatttt gaaaccaatt ccccagatca
     3061 ggtccgttcc tgcgtcgagc agggtttcaa ggtttgggcc gtagtcggcg tcttgacgcg
     3121 attctatata ggctgccttc acacccagat ctttttcagc tctttgaagc ccttcccatg
     3181 ctgattggtt gaaggattga tcgttcacgc cccctacgtc tgtgatcatt gcaatcgata
     3241 gttgtgatgc ccccgaagac agcgcaaaaa caagaaggac aatcaaagcg aacagcaccc
     3301 gtaaagcttt cattagacca actcctctct tgggatagta ttacgtgccg gaagaaaaac
     3361 cggcttcaat atttcaacaa tcttacatcc ctggaaaaat ttccgcaagc gccatccgcg
     3421 gctctcgaag cgtgttttca atgggataat cagttttgat gaaaagagga tactgtaaag
     3481 agcagattgt ctttgtgtga aggagggtag tgtgta
//
LOCUS       DAOSWW010000173         3496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19090_length_3496_cov_3.032549, whole genome shotgun sequence.
ACCESSION   DAOSWW010000173 DAOSWW010000000
VERSION     DAOSWW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19090_length_3496_cov_3.032549"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2972)
                     /locus_tag="PK442_04745"
     CDS             complement(<1..2972)
                     /locus_tag="PK442_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO86987.1"
                     /translation="MRRERVIKQLGRSLPAFSAAALLFLCIAVCAACLFAFPSDASLM
                     EDFRSSGEAVELSGEERDYLAALGPVTVCPDPDWMPFEHVDEQGNFTGIAADLLELIA
                     GRLGIEFSYVFPKDWSEAVALSKSGDVLILPFLNKTPAREEWLLFTEPLLVDPNVFIA
                     REEHPYIYDASQLMGVLKSEPRLRDILNKGIAAITPSELEAIVNRHVNITVVRQMDYW
                     FVIRIAAALGLFVAFSFYWNARLRKSRAALEESERSKSVLIANLPGIAYRCRYDEEWT
                     MEFVSEGCLRLTGYASDDLLFNKKISFNKLMLPEYREYARAVWERAMAERVPAALEYR
                     ILTAEGEEKWVFEQGVPLFDETGAVEFLEGLIIDISSRKALEDRLQSTLGLQGLLAEI
                     SASFINATLANIDEKIDAMLERCGEFLKVDRTFLFQLSEDCTLMSNTHEWCAPGIPAV
                     KDDVQNYPVADVPLIEKTICAQEMLFIPDVSELSEEWSAEKRELERRQVRSVLCLPLI
                     KGGRFLGYFGFDAVRATKQLDDETVEVLKILANTLADALESNRIEKEMLEAKTRAEDA
                     TRAKSEFLANMSHDIRTPMNGVLGMTALLLDTQLSEEQRRFAETVRSSAESLLGILND
                     ILDFSEIEAGKLDLENLDFNLSALLDDLLAGLKVHAEQKGVTLRSDAEQDVPAILRGD
                     PGRLRQILVNLVGNAVKFTHEGSVDVRVSRAEERDEGVLLRFSVKDTGIGIPEEKLGA
                     LFDKFSQADASTARKYGGAGLGLAISKQLAELMGGSVGATSTEGKGSEFWFTALFDRS
                     MEGADDNALAPSSGAAGFKNAKNSRMSRPRFERRGIRILLAEDNLTNQQVAVGILKKF
                     GLWTDVVADGEEALAALENVRYDLVLMDLQMPRMDGYEASRRIRAREGGARRVPVIAM
                     TAHTMVGDREKCLDAGMDDYIAKPISPEVVLDVLERWLPAGESGAHEYVSVTEGARLE
                     GGAQPVSPQAPFSPSAWDS"
     gene            complement(2972..>3496)
                     /locus_tag="PK442_04750"
     CDS             complement(2972..>3496)
                     /locus_tag="PK442_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOO86988.1"
                     /translation="DTLKKWLPEREKERTSPEKEGGPPVAKPKKTNAPKQKKTALRVP
                     AAGAPAAPEQQAWNRGLLLERLMGDEELVMEVLQDFLSELPRELRKLGDVLSSDNLPL
                     MERHAHSIKGMSACTGAEALASVAFKIEERAREEDAEGARSFLPALKKEWKRLKTELE
                     EERAPRNHSRRTRT"
BASE COUNT          651 a         1106 c         1007 g          732 t
ORIGIN      
        1 gagtcccagg ccgaaggcga aaagggcgcc tgcggagaaa caggttgcgc gcctccttcg
       61 agccgcgcgc cttcggtgac ggacacatac tcgtgagcgc cgctttctcc ggcgggcaac
      121 caccgttcga gcacgtccag cacaacctcc ggagaaatgg gttttgcgat atagtcgtcc
      181 attccggcgt ccagacactt ctccctgtct ccgaccatcg tgtgcgccgt catggcgatg
      241 acgggaacgc gccgtgcgcc gccctccctc gcgcgaattc tgcgtgacgc ttcgtagccg
      301 tccatccggg gcatctgcaa gtccatcagc acgaggtcgt aacgcacgtt ctccaatgcg
      361 gcgagcgcct cttcgccgtc cgcgacgacg tccgtccaca ggccgaattt tttcaggata
      421 cccacggcga cctgctgatt ggtgaggtta tcctcggcaa ggaggatgcg gattcccctt
      481 cgttcaaacc ggggacgcga catccgggag ttcttcgcgt tcttgaagcc ggcggccccc
      541 gaagagggag caagcgcatt gtcatcagcg ccttccatgc tccgatcaaa aagcgcggtg
      601 aaccaaaact ccgacccctt tccttccgtg ctcgttgcac cgacggaacc tcccatcagt
      661 tcggcaagct gcttgctgat ggcgagcccc agcccggcgc cgccgtactt ccgtgccgtc
      721 gaggcgtccg cctggctgaa cttgtcgaag agggctccga gcttctcctc agggatgccg
      781 attccggtgt ccttcaccga aaaacggagc agcacgcctt cgtcgcgttc ttcggcacgc
      841 gacacgcgga cgtcgacgct tccttcgtgg gtgaatttga ccgcgttccc gacaagattg
      901 acgaggatct ggcgcagacg tccggggtcg ccccgaagta tcgcgggaac gtcctgctcc
      961 gcatcgctgc gaagcgtcac ccctttctgt tccgcatgca ccttcaaccc ggccagaaga
     1021 tcgtccagga gagcggagag gttgaaatca aggttctcca gatcgagctt tccggcttcg
     1081 atctcggaga aatcaagaat atcgttgaga atgccgagga gcgactccgc gctcgaacgg
     1141 acggtttcgg cgaaccggcg ctgctcttcg ctcaactgtg tgtccagcag aagcgccgtc
     1201 atgccgagca ccccgttcat cggcgtgcgg atgtcgtggc tcatgttggc gaggaattcg
     1261 ctcttcgcgc gggtcgcgtc ctcggcgcgg gttttggcct ccagcatctc tttttcgatc
     1321 ctgttgctct ccagagcgtc ggccaatgtg ttcgcaagga tcttgagcac ttcgacggtc
     1381 tcgtcgtcga gctgcttcgt cgcgcggacc gcgtcgaaac cgaaatatcc gaggaaacgg
     1441 ccgcctttga tcagcggcag acagagcaca gaacgcacct gccggcgttc gagctcgcgc
     1501 ttctccgcgc tccactcctc cgacagctcc gagacgtccg ggatgaagag catctcctgt
     1561 gcgcagattg ttttctcgat cagcgggacg tcggcgacgg ggtagttctg cacgtcgtcc
     1621 ttcaccgcag gtattccggg agcgcaccac tcgtgcgtgt tggacatgag cgtacagtcc
     1681 tccgaaagct ggaagaggaa ggttctgtcc accttgagaa actcgccgca acgttcgagc
     1741 atggcgtcga tcttctcgtc gatgttggcg agcgtggcat tgatgaaaga ggcggaaatc
     1801 tccgcgagca gcccttggag tccaagagtg ctttgaagtc gatcctcaag agctttccgc
     1861 gaagagatgt cgatgatgag tccttcaaga aactcgaccg ctcccgtctc gtcaaagagg
     1921 ggaacgccct gttcgaagac ccacttctcc tccccctccg ccgtcaggat tctgtactcc
     1981 agcgccgccg gaacgcgctc cgccatcgct cgttcccaga cggcgcgggc gtactccctg
     2041 tactccggca gcatcagctt gttgaacgag atttttttgt tgaacagcag atcgtcgctg
     2101 gcgtacccgg tcaaacggag acacccttcg gagacgaact ccattgtcca ctcttcgtcg
     2161 tagcggcaac gataggcaat ccccggcagg ttggctatca gcactgattt gcttcgttcg
     2221 ctctcttcaa gcgcggcgcg gcttttccgg aggcgcgcgt tccagtagaa ggagaaagcg
     2281 acgaaaagcc ccagggccgc ggcaattctg atgacgaacc agtaatccat ctgccggacg
     2341 accgtgatgt tcacatgtcg gttgacgatc gcctccagct cgctcggcgt gatcgcggca
     2401 attcctttgt tcaggatgtc gcgcaaccgc ggctcgctct tcaggacgcc cattagttgc
     2461 gaagcgtcgt agatataggg gtgttcttcg cgggcgataa agacgttcgg gtccaccagc
     2521 agcggttcgg tgaagagaag ccattcctcc cttgcgggag tcttgttcag gaagggaaga
     2581 atcagaacgt cgcccgactt ggagagcgcc acggcctcgc tccagtcctt cgggaaaacg
     2641 taggagaatt caatgccgag gcgtccggcg ataagctcca gcagatccgc cgctattccc
     2701 gtgaagtttc cctgttcgtc gacgtgctcg aacggcatcc agtccggatc gggacagact
     2761 gtgacgggac cgagcgccgc cagatagtcc cgctcttccc cggagagttc gacagcctcc
     2821 cctgacgagc ggaagtcctc catgagcgac gcatccgagg gaaaagcgaa gagacaggct
     2881 gcgcacacgg cgatgcacag gaaaagaagc gcagcggcag agaacgccgg aagagaacgc
     2941 ccaagttgct tgatgacgcg ctcccgtcgc attacgttct cgtcctcctg ctgtgattgc
     3001 gcggcgctcg ttcttcttcc agctcggttt tcagccgctt ccactccttt tttaacgcgg
     3061 gaaggaagct tcgcgcaccc tccgcgtctt cttctctcgc ccgctcctct atcttgaaag
     3121 caacggaggc gagagcctcg gccccggtgc atgcgctcat ccccttgata ctgtgcgcat
     3181 gccgctccat caaggggagg ttgtcagagg agagcacatc cccaagcttc cggagttcgc
     3241 ggggaagctc cgacaggaaa tcctgaagca cttccattac aagctcctcg tctcccatca
     3301 ggcgctccag gagcagcccc ctgttccagg cttgctgctc cggtgcagcg ggcgcgccgg
     3361 ccgccggaac gcgaagagct gtcttctttt gcttcggcgc gtttgtcttc ttcggcttcg
     3421 cgaccggagg cccgccctct ttctccggag acgttcgttc tttttcacgt tcaggaagcc
     3481 attttttaag cgtatc
//
LOCUS       DAOSWW010000174         3477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19250_length_3477_cov_3.973407, whole genome shotgun sequence.
ACCESSION   DAOSWW010000174 DAOSWW010000000
VERSION     DAOSWW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19250_length_3477_cov_3.973407"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..2298
                     /locus_tag="PK442_04755"
     CDS             439..2298
                     /locus_tag="PK442_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOO86989.1"
                     /translation="MYGYWGKLLRVDLTHRTHRIEDIPEEVFDLLLGGSALGAKILLE
                     ETPANVDPFSPENKLIFSVGPFQGVNFPGNGKWSVITKGPLTGTYLESAGTGHWAPYF
                     KKCGYDAVVFEGKASSPVYVVLRDDSVEFRDASFIWGKDTVETGELIKEDLGDKRINA
                     LNIGPAGEIGNPIACITCDGHSFAGRGGAGAVMGSKNLKAVAAWGTKEVPVFDKEKAS
                     EYSKEIFKLLHEQGEGFRQHGTPVVMVPLEEIGDVPIKYWRGDENKYGAYLLGAPRYT
                     EYLNVKPLPCINCPVGCHRHIHFEFPDGTVLDGNGPEYETIGMMGTNLLMEDLSAVAV
                     ANDIANRMGIDTVSCGAWIGFLMECWEYGWITPKETDGVTIKWGDPEVLVELTQQIAS
                     MSGVGEWFREGVRGAAKKIGKDAEKITVEVKNLDYPAHDPRAVYGLGVNYAVGTRGAC
                     HERGNPQASALGLFFPELGQDAPYDRQDPKFAAKTAILHQDVSALHNCMTLCKFMMGG
                     AGLTLTEMNTAFKHLTGKDVPVPEFVKYGERAWQLQRLINVRDGMNRKDDTLPLKMTI
                     PAMVGPRAGKAPTPHDEILDDYYAQRGWDKNGVPTKERLEALGLGAYVKYLGR"
     gene            2324..>3477
                     /locus_tag="PK442_04760"
     CDS             2324..>3477
                     /locus_tag="PK442_04760"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOO86990.1"
                     /translation="MMQAKVGWSTAGSPLAAGKEAAQAAKVDGAQIALLFGSVDYNAE
                     ELLKGVRETLGDVPVIGCTSYTGVLTPGGFITSPDGYCALMALSDEDMAVSVGAAEKK
                     ECAVAAGEDAALAALEAAGMDMAPEFFYMVAPPGEEEFYLQGIQSVIGRVPFFGGSAA
                     DNDLSGKWLVYGNGVVKNGVAVAFFWNKAYGNKYTGAYRGTGKRGIITKVENNRVLKE
                     IDGKPALDVLAGWLGSTPADLQGFNLLGATIHHPIGITDFAGGHVWIRHPMVGNADGS
                     ANIGNNLAEGVAIELMEATTDELVASVESTAKAAVEMLGDEGLGALVAVHCGGRRGGI
                     GDRMDETVTAMKNAIGETPFIGVFTFGEYGFEGDLANGCGGLMLSFMALG"
BASE COUNT          804 a          916 c         1106 g          651 t
ORIGIN      
        1 agctgtggtc gaagaagtga agtctgaacc tgtttccggg gcgcctgttg ctgtggagga
       61 accgaagcct gaagaacccg ctgccgaagc ggagtaggaa acagtagtgg aagaggcggc
      121 aaaagaagcg gcagaggttc cggcgcctga gagccctgcc ccggagaccg tacagcagga
      181 caagcagacg gaaaagccgg caggggcaac agcagctccc tgcgactgag ctccagaaca
      241 ctcaccaaaa gaacctgttt tgattgcaac aagaagagat tcattgaaaa gcctccgtgt
      301 ttcctgcgga ggcttcttcg tacaaaaaaa atataatagt tgcaaagttg gagctttgcc
      361 tgtagactga tattgtttgt tgaactgttc atctaataga gttccaacat actgtaccga
      421 ttttcaggag gtggagcaat gtacgggtat tggggtaaac tgcttcgggt cgacctgaca
      481 catcggacgc acagaatcga ggatattccc gaggaggtct tcgatctgct gctgggcggc
      541 tctgcgctag gagccaaaat tctgctggag gaaactccgg cgaacgtgga tcccttttca
      601 ccggagaaca agcttatttt ctccgtcggt ccgttccagg gggtgaattt cccggggaac
      661 ggcaagtgga gcgtgatcac caaaggtcct cttaccggaa cgtacctgga gtcggccggt
      721 accggacact gggcgcccta cttcaagaag tgcggctacg atgcagtagt cttcgaagga
      781 aaggcgagca gccctgtcta tgtagtgctc cgcgacgatt ccgtcgagtt ccgcgacgcg
      841 tcctttatct gggggaagga caccgtcgag accggcgaac tgatcaagga ggatctgggc
      901 gacaaacgta tcaacgcgct caatatcgga cctgccgggg agatcggcaa tcccatagcc
      961 tgcattacct gtgacgggca cagtttcgca ggcaggggag gcgcaggcgc ggtcatgggt
     1021 tccaagaact tgaaggccgt tgccgcatgg gggacgaagg aagttcccgt tttcgacaag
     1081 gagaaggctt ccgagtactc caaggaaatc ttcaagctcc ttcacgaaca gggggagggg
     1141 ttccgtcagc acggaacgcc ggttgtcatg gtcccgcttg aagaaatcgg cgacgtgcct
     1201 atcaagtact ggcgcggaga cgagaataaa tacggggcct acttactggg agctccccgc
     1261 tacaccgagt atctcaacgt aaaacctctg ccgtgtatta actgtccagt cggctgtcat
     1321 cgtcacatcc atttcgagtt tccggacgga acggtactcg acggcaacgg tcccgagtac
     1381 gaaaccatcg gcatgatggg aacgaacctg ctcatggaag acctgagcgc cgtcgccgtc
     1441 gccaacgata ttgcaaaccg catggggatc gacaccgtct cctgcggcgc atggatcggt
     1501 tttctcatgg agtgctggga gtacggctgg atcacgccga aagagaccga cggcgtcacg
     1561 atcaagtggg gagatcctga agttctcgtc gaattgacgc agcagatcgc ctccatgagc
     1621 ggcgtaggag agtggttccg cgagggcgtc cgcggcgcgg cgaagaagat cggcaaggat
     1681 gcggagaaga tcacggttga ggtgaagaat ctcgactatc cggcgcacga ccctcgcgca
     1741 gtctacggat tgggcgtgaa ctatgccgtc ggaaccaggg gagcatgtca cgaacgaggc
     1801 aatccccagg cgagcgcgct tggactcttc ttccccgaac tgggacagga tgctccgtac
     1861 gaccgccagg atccgaagtt cgcagcaaag accgcgatac ttcatcaaga cgtgagcgcg
     1921 ctgcacaact gcatgacgct ctgtaagttc atgatgggcg gcgcagggct tactcttacc
     1981 gagatgaata cggccttcaa acatcttacc ggaaaggacg ttccggttcc cgagttcgtg
     2041 aagtacggcg agcgcgcctg gcagcttcaa cgcctcataa acgtccgcga cggcatgaac
     2101 cggaaggacg acacgctccc gttgaagatg accatcccgg cgatggtagg gccaagagca
     2161 ggaaaggcgc cgactcctca cgacgaaata ctggacgatt actatgcgca gcgcggatgg
     2221 gacaagaacg gcgtcccgac gaaggaacgc ttggaagcgc tcgggcttgg agcatacgtg
     2281 aagtatttgg gccgataggc ggagcgggga ggtcgagaag attatgatgc aggcaaaagt
     2341 tgggtggagt acagccggat cgcccttggc tgcgggaaaa gaggcggcgc aggcggcgaa
     2401 ggtcgacggc gcgcagatcg cgttgctctt cggaagcgtc gattacaacg ccgaggagct
     2461 cctgaaagga gtgcgtgaaa cgctgggcga cgttcccgtc atagggtgta catcctatac
     2521 cggcgttctt accccgggag gttttatcac atctcctgat ggctactgcg ctctgatggc
     2581 tctctcggat gaggatatgg cggtttccgt cggagcggcg gaaaagaaag aatgcgcggt
     2641 tgccgcggga gaagatgcgg ctttggccgc gctggaagcc gccggtatgg atatggcgcc
     2701 cgagttcttc tacatggtcg cccctccggg agaggaagag ttctaccttc agggaattca
     2761 gtccgtcatc ggcagagttc ctttcttcgg cgggagcgcg gccgacaacg acctgtccgg
     2821 aaagtggctc gtctacggca acggcgtggt caagaacggg gtcgcggtgg ctttcttctg
     2881 gaacaaggcg tacggcaaca agtacacggg cgcctacaga ggcacaggaa agcgcggcat
     2941 catcacgaag gtggagaaca accgagttct caaggagatc gacggcaaac ctgcgctcga
     3001 cgtgctcgcc ggatggcttg gaagcacccc cgccgatctg caggggttca acctcttggg
     3061 agccaccata catcacccca tcggcatcac cgatttcgcc gggggacacg tctggatccg
     3121 ccatccgatg gtcggcaacg cggacggcag cgcgaatatc ggaaacaacc tcgccgaggg
     3181 cgtggcaata gagcttatgg aggcgactac cgacgagctt gtggcgtctg ttgagagcac
     3241 cgcgaaagcc gccgtggaaa tgctcggaga cgaggggctc ggagcgctcg tggccgttca
     3301 ctgcggcgga cgtcgcggag gtatcggcga ccggatggac gaaaccgtaa cggcgatgaa
     3361 aaacgccata ggcgagactc cgttcatcgg tgtcttcaca ttcggcgaat acggctttga
     3421 aggagatttg gccaacggtt gcggcggcct catgctctcc tttatggcgc tggggaa
//
LOCUS       DAOSWW010000175         3477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19252_length_3477_cov_3.543250, whole genome shotgun sequence.
ACCESSION   DAOSWW010000175 DAOSWW010000000
VERSION     DAOSWW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19252_length_3477_cov_3.543250"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PK442_04765"
     CDS             complement(<1..953)
                     /locus_tag="PK442_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOO86991.1"
                     /translation="MIGKILRWAIVLALLVILISGSVAFYTVFFGGKDLVIPPLREMS
                     VLDAVESAERMGLEVRIEQIDSSVPVGTVLAQWPDPGTKVRKDKTVILKVSKGGNRQA
                     ITDLRGLEQGQALRMIEEQGFSAGEILRVHDGNRPAGTVIAQNPASPAMIEGGRKIEL
                     LISMGPLPKDGRIPVPDLAQRDEKTARELLLQSGLRVGGVEYVNTQHTPDGMVMATRP
                     KTGAMIRQGDAVVIQVATSRRKEPELVPETRAATPTPPPQGEVVVEQPQTPVRQIGPA
                     GPVQTLTPEQQELAGAIGLPVDRPPAAATPTPSTPPAVRPGP"
     gene            complement(950..2281)
                     /locus_tag="PK442_04770"
     CDS             complement(950..2281)
                     /locus_tag="PK442_04770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOO86992.1"
                     /translation="MRGIEAALHVWSEVREGKFASEVIRRLADSMTPGDLMLASTLVY
                     TALRRQFLWKEIYAHFLKASPAGVSRLTDDALCIGTAGLLELRKFAPRVLVNALVQYM
                     KDKGDERGARLVNAVLRRVSEEGRNELARLESSGNSKEQALVAGIPPWVASYWRNTIG
                     DEAKKLLKMARIRPYSSFRILPEDKMDAILEQAKNSGICCWPSPLVPYSIRLSSTIYP
                     GNFPGYSEGWSTPQSESSMLVGEVMKKVYKGGAILEMCSGRGVKTGHIARLFPEAHIE
                     CVELSQGRVGAAVREMKRLNIRRLPVFTAADALHAEPASSPAFISLDAPCSGSGTWNR
                     HPEGKWRLSAEKLESLALLQRNLLKRAVSLLAPGGIVVYSTCSLLKTENENVVAGILS
                     EDASLVELSFPFSAKYFRRGRPWGVYIWPELPWLDGFYMAVIMKRSGGKDC"
     gene            complement(2278..3033)
                     /locus_tag="PK442_04775"
     CDS             complement(2278..3033)
                     /locus_tag="PK442_04775"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HOO86993.1"
                     /translation="MVWEMERLSRSLHFISSGRKDQNPMPLLFFLIFIFIFPWFFLLL
                     VLIFFVLIPLGFSIQSFLWILTRPAQLLRIVTSRQVRKNHALAHGTLRILEEEYGRLN
                     AEGMPVEDGFSLRGGVDPETALAAAKTALDRIRRGDRSLAFFHRCGITVVMANTLFAF
                     ILFFVLRMLGLFSFLSAILCLVAVYFAGSRLSYLAQVYGTISPDLDDMEITGVDVGNE
                     QFALGGLSFLTPSSIFIRTRRAGEPVIAEVVSP"
     gene            complement(3061..>3477)
                     /locus_tag="PK442_04780"
     CDS             complement(3061..>3477)
                     /locus_tag="PK442_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOO86994.1"
                     /translation="AAIGVAAHEVGHAIQDSVNYSPLKIRNAIVPVVGIGSTLAFPLF
                     FIGILLRGQMMMDLGIVLFLGVILFHLVTLPVEFDASNRALRVLADTGMLSTDEIGGA
                     GAVLRAASWTYVAATVMAFAQLIRLLFLRGMYGSRD"
BASE COUNT          922 a          979 c          836 g          740 t
ORIGIN      
        1 ggtcctggac gaacagcggg aggggtagaa ggcgtcggtg tcgctgctgc cggcggtctg
       61 tctacaggca aacctatggc tccggcaagt tcctgctgct ccggggtcaa tgtttgtaca
      121 ggcccggccg gtccaatttg gcgaacgggc gtctgcggtt gttctacaac gacttcaccc
      181 tgcgggggcg gagtcggcgt ggccgctcga gtttccggaa ccagttccgg ttccttccgc
      241 ctgcttgttg ccacctgaat aacaactgcg tctccctggc gtatcattgc cccagttttc
      301 ggcctggtcg ccatgaccat accatccggg gtgtgctgcg tattcacata ttcaacaccg
      361 cccacgcgaa gaccgctttg cagcaaaagt tctcttgcag ttttctcatc gcgttgcgct
      421 aaatccggaa cgggaatacg tccgtctttg ggcagaggcc ccatgctgat aaggagttca
      481 atttttcttc ctccttcaat catagcagga gaagcgggat tctgagcgat tacagtgcct
      541 gcaggacgat ttccgtcgtg gacacggaga atctcacctg cgctgaaccc ctgctcttca
      601 atcatccgca acgcttgccc ctgttctagt cctcgcaaat cagtgattgc ttgcctgttt
      661 ccgcccttac tgaccttcag tataaccgtt ttatcttttc gcacttttgt accgggatcc
      721 ggccactggg caagaaccgt accgacggga acagatgaat caatctgttc aattctgact
      781 tccaagccca tgcgctcagc gctttctacc gcatcaagca ctgacatctc ccgcagcggc
      841 gggatcacca ggtctttccc tccaaaaaaa acagtgtaaa aagcgacgct tccggagata
      901 agaatgacca acaacgccag tactatggcc cagcgtaata tttttcctat caacagtctt
      961 tccctcccga tctcttcatg atcaccgcca tgtaaaaacc atcaagccaa ggcagttcag
     1021 gccagatgta gactccccag ggacgtcctc tacgaaagta tttcgcggaa aaaggaaaag
     1081 aaagttcgac aagagaagcg tcctccgaaa gaattccggc gactacattt tcattttctg
     1141 tcttcaaaag actacatgta gagtagacca caatccctcc cggcgcaaga agagaaactg
     1201 ctctttttaa aaggttccgc tgaagcaggg ctaaagactc caatttttct gcggaaaggc
     1261 gccacttgcc ctccggatgc cgattccatg tgccgcttcc tgaacaggga gcatcaagtg
     1321 aaatgaaagc gggagaactc gcaggttctg catgcagggc gtccgccgca gtaaaaacag
     1381 gaagacgtct gatattgaga cgcttcatct cccggaccgc ggctccgaca cgtccttgag
     1441 acagttccac gcactcaatg tgagcttcgg gaaaaagtcg cgcgatgtgt ccggttttca
     1501 cgccgcgtcc agagcacatc tccaaaatag ctccgccctt gtatactttt ttcatcacct
     1561 cgccgacgag catcgaggac tccgactgag gcgtagacca tccctcggaa taccctggga
     1621 agttgcccgg gtatatggtg gagctgaggc gaattgaata cgggacaaga ggggaaggcc
     1681 aacagcaaat gccgctgttt ttagcttgtt ccaggatagc gtccattttg tcctccggaa
     1741 gaatgcgaaa cgaagaatat ggacgaatac gcgccatttt caggagtttt tttgcctcat
     1801 cgccgatagt gttcctccaa tacgaagcaa cccacggggg gatacctgcc acaagcgcct
     1861 gttccttgct gttgccggaa gactcgagcc gcgcaagttc atttctccct tcttcagata
     1921 cccttcgaag aacggcgttc accagccgag cgcctctttc gtccccttta tctttcatat
     1981 actgcacaag agcatttacc aaaacacgcg gagcaaactt cctcagctca agcaaacctg
     2041 cggtcccaat gcaaagagca tcgtctgtaa gacgagagac gccggcggga gacgccttca
     2101 aaaaatgcgc gtagatctct ttccagagga actgcctcct gagggctgta tacacaaggg
     2161 tcgacgcaag cataagatct ccaggcgtca ttgaatctgc caggcgtctg ataacctcgg
     2221 aagcgaattt gccttcccgc acttcactcc agacatgaag agcagcctca atccctctca
     2281 aggcgagacc acctcggcaa tcacaggctc gccggctcgt cttgtccgaa taaaaatact
     2341 tgatggagta agaaaggaga gcccccccag cgcaaattgc tcgtttccta catccactcc
     2401 cgtaatctcc atatcatcca gatcaggcga aatcgtgccg taaacttgag cgagataact
     2461 gaggcgagat ccggcaaagt acaccgctac aaggcagaga atagctgaaa ggaaactgaa
     2521 cagaccaagc atgcgcaaca caaaaaaaag gatgaaagca aaaagcgtgt ttgccatcac
     2581 gacagttatc ccgcaacgat gaaaaaacgc cagcgaccga tcaccccttc ggatacgatc
     2641 caaagcagtc ttcgcagccg cgagtgcagt ttccggatcg actcctcctc tgagagaaaa
     2701 accgtcttcg acgggcatac cttcagcgtt caatcgtccg tactcctctt caaggatacg
     2761 gagcgtgcca tgagcaaggg cgtggttttt gcgaacctga cggcttgtca caatacgcaa
     2821 aagctgtgca ggccgtgtaa gaatccagag aaacgactgg atcgaaaaac caagaggaat
     2881 aagcacaaaa aagatgagga caagcagcag aaaaaaccaa gggaaaataa aaataaaaat
     2941 caagaaaaaa agcaaaggca tgggattctg gtcctttctc cccgaggaaa taaaatggag
     3001 cgaccgggaa agccgctcca tttcccagac catcgcctct cggcgaagct gaatacgttt
     3061 ttagtctctg cttccataca taccacggag aaagagaagc cgtataagct gggcgaatgc
     3121 cattacggtc gccgcaacat aggtccacga tgctgcgcgc aaaaccgctc ctgctcctcc
     3181 tatttcatcg gttgacagca ttcccgtatc ggcaagaaca cggagcgcgc gattacttgc
     3241 gtcgaactca accggcaagg taaccagatg gaagagtatc accccaagga agagaacaat
     3301 cccaagatcc atcatcatct gcccccgaag aagaattcct atgaagaaca ggggaaaggc
     3361 aagagtcgag ccgatcccga caaccggcac aatagcattc ctgatcttca aaggtgagta
     3421 attcacactg tcctggatcg catgcccgac ttcgtgtgcg gcgactccaa tagcagc
//
LOCUS       DAOSWW010000176         3460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19382_length_3460_cov_3.859031, whole genome shotgun sequence.
ACCESSION   DAOSWW010000176 DAOSWW010000000
VERSION     DAOSWW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19382_length_3460_cov_3.859031"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /gene="nuoF"
                     /locus_tag="PK442_04785"
     CDS             complement(<1..1687)
                     /gene="nuoF"
                     /locus_tag="PK442_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOO86995.1"
                     /translation="MTIFRSHVLICGGTGCTSSGAAEVIGSFKQELARRKLDGEVLVV
                     PTGCHGMCEMGPIVVVYPEGTFYCKVTANDVPEIVEEHIYKGRPVQRLMYEGSGDMPA
                     LPHYKDIPFYGKQHRIALANCGYINPENIDEYIARGGYEALGKAITSMSPAQVIDEVK
                     TSGLRGRGGGGFPTGLKWSFCAAAGGEKKYVICNADEGDPGAFMDRSILEGDPHSVIE
                     GMMLGAYAMGADEGYIYCRAEYPLAIERLKQAITAAEGYGLLGDSIMNSSFSFHLHIK
                     EGAGAFVCGEETALMGSIEGRRGMPRPRPPFPANSGLWGYPTNINNVETWANVPQIIS
                     NGGEWYASMGTEKSKGTKVFALTGKVKHTGLVEVPMGITLREIVFDIGGGILNDKKFK
                     AVQIGGPSGGCLTEEHLDTPVSYESLTALGAIMGSGGLVVMDEDNCMVDVAKFFLEFT
                     QKESCGKCVPCREGTKHMLLLLEKITEGKGTMEDLDTLEELAHMVRDMSLCGLGQTAP
                     NPVLTTLRYFREEYIAHIRDKRCPAGACTALLRYTILQDVCKKCGICAKNCPVH"
     gene            complement(1706..2080)
                     /locus_tag="PK442_04790"
     CDS             complement(1706..2080)
                     /locus_tag="PK442_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOO86996.1"
                     /translation="MEQKKITSLDDLRKIRENSKDLSSARSGGGTTVIVGMGTCGIAA
                     GAREILNEVMSELARRNLDDIAVQTTGCIGMCQDEPLLDVIRSGQRVTYGRVKPEDVP
                     TIIAEHVVNGRVVEKLAIGRAD"
     gene            complement(2097..2567)
                     /gene="nuoE"
                     /locus_tag="PK442_04795"
     CDS             complement(2097..2567)
                     /gene="nuoE"
                     /locus_tag="PK442_04795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOO86997.1"
                     /translation="MAFSVEEIREKTRKITEPWRERNGGLIPILQAVQAQLGYLPQEA
                     LETISRELKIPPSEIYGVATFYAQFHLKPRGRHVVRVCRGTACHVRGSLKILEKVKNL
                     IGVGENETTEDLRFTIEPVACLGACGLAPVMMVDTQTFGRLVPDMVKGILDKFE"
     gene            2759..>3460
                     /locus_tag="PK442_04800"
     CDS             2759..>3460
                     /locus_tag="PK442_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOO86998.1"
                     /translation="MARIEYVNIEDALGMALSHDMTQIDVKSGYKGARFKRGHIVKPE
                     DLPVLRSMGKNSISVLYLEEGDVHEDDAALRLAHRLAGEGLRMEGPEEGKVSLFAEWN
                     GLIVYDEESIHTINEDVDWIAVTTPNKVPVKKGEPAAGIRVVPLTMREEQIRKAEAAA
                     LPLTVFPFHPLKTALVTTGREIVEGRIRDAFAPKLVRKLAFYGSTLMEQVITGDEKDE
                     IARAIQSFIDKGAELI"
BASE COUNT          740 a         1071 c          896 g          753 t
ORIGIN      
        1 agtggacagg gcagttcttg gcgcagatac cgcacttctt gcacacgtct tgaagaatag
       61 tgtaccggag cagcgcggta caggccccgg ccgggcatct cttgtcccgg atatgcgcga
      121 tatactcctc cctaaagtag cggagggtcg tcagcaccgg gttcggcgca gtctgcccga
      181 gtccgcacag ggacatgtcg cgcaccatgt gcgcaagctc ttccagcgtg tcgagatcct
      241 ccatggttcc cttgccctcg gtgatcttct ccagaagcag cagcatgtgc ttcgttccct
      301 ctctgcaggg aacgcacttg ccgcatgact ctttctgcgt gaattcgagg aagaacttcg
      361 ccacgtcgac catacagttg tcctcgtcca tgaccaccag cccgcctgat cccatgatcg
      421 cgccgagcgc cgtaagcgac tcgtagctca ccggagtgtc cagatgctcc tcggtcagac
      481 agcctccgct gggaccgcct atctgcacgg ccttgaactt cttgtcattg agaatgccgc
      541 cgccgatgtc gaaaacgatt tcccggaggg taatgcccat cggtacctcg acgagcccgg
      601 tgtgcttcac cttgcccgtc agggcgaaga ccttcgtccc tttcgacttc tccgttccca
      661 tcgaggcgta ccactcgccg ccgttactga ttatctgcgg aacgttggcc cacgtctcga
      721 cgttgttgat gttcgtcgga tacccccaaa ggccggaatt cgccgggaaa ggaggtcgag
      781 gacgaggcat gccgcgccgc ccttcgatgg accccatcag cgcggtctcc tcgccgcaga
      841 cgaacgctcc ggcgccttcc tttatatgca ggtgaaagga aaaagagctg ttcataatgc
      901 tgtcgccgag cagtccgtac ccctcggccg cggtgatcgc ttgtttcaga cgctctattg
      961 ccagcgggta ttccgcccgg cagtaaatgt acccctcgtc ggcccccatc gcataggcgc
     1021 cgagcatcat gccctcgatc acgctatgag gatcaccctc aagaatggaa cggtccatga
     1081 acgcgccggg atcgccttcg tcagcgttac agataacata ctttttctcc ccgccggcag
     1141 cggcgcagaa actccatttc aaccctgtgg ggaatccccc gccccctctt ccgcggaggc
     1201 cggaggtttt cacctcatca attacctgcg ccggagacat cgaggtgatc gccttgccga
     1261 gggcctcgta ccctcccctg gcgatatatt cgtcgatatt ctccggattg atatagccgc
     1321 agttcgcaag ggctatcctg tgctgcttcc cgtagaaagg tatatccttg taatgcggca
     1381 atgcgggcat atcgcccgaa ccttcgtaca tgaggcgctg caccggacgt cctttgtaga
     1441 tgtgttcctc gacgatctca ggaacatcgt tcgccgttac cttgcagtag aacgttcctt
     1501 cagggtacac gacaacgatg ggccccatct cgcacatgcc gtgacatccg gtcgggacga
     1561 ccaggacctc tccgtcgagc ttccgccgcg caagctcctg tttaaaggag ccgatgacct
     1621 ccgcggcgcc gctggaagta catccggtac cgccgcagat cagaacatgg gatcggaaaa
     1681 ttgtcattcg tccttctcct tccgctcagt cggcccgccc gatggccagc ttctccacga
     1741 cgcgtccgtt cacgacgtgt tccgcgatga tcgtcgggac atcctccggt ttcacgcggc
     1801 cgtacgtcac tctctgccct gaacggatca catcgagcag gggttcgtcc tggcacatgc
     1861 cgatacaccc cgtcgtctgc accgcaatat catcgaggtt acgcctggca agctccgaca
     1921 tcacctcgtt gagtatctcc cgagctcctg ccgcgatacc gcaggttccc ataccgacaa
     1981 taacggtcgt tccgcctccc gaacgcgccg aggagaggtc tttcgagttt tcgcgtatct
     2041 ttcgaaggtc atcgagactc gtgatctttt tctgctccat tcgctcttcc cccgttctac
     2101 tcgaacttgt caagaatgcc cttcaccatg tcgggaacga gccttccgaa tgtctgcgta
     2161 tcgaccatca ttacaggggc aaggccacag gcgccgagac aggccacagg ctctatagta
     2221 aaccggagat cctcagtggt ctcgttctcg ccaaccccga taagattctt caccttctcg
     2281 agaattttga gacttccccg aacgtggcaa gccgtccccc tacagacgcg cacaacatgt
     2341 cgtccccgag gcttcaggtg aaattgggcg taaaacgtcg caacgccgta aatttcagac
     2401 ggcgggatct tcagctcccg ggaaatagtt tccaacgcct cttgcggcaa atacccaagc
     2461 tgcgcctgaa cagcctgtaa aatcgggata aggccgccgt tcctctctct ccagggttct
     2521 gtaatctttc gtgtcttctc gcggatttct tcaacgctga aagccatgat tcccctcctt
     2581 tttcaacaga acataggatt gtgcacaata tcacaatgaa aaatatcata tcataggcag
     2641 gaaccaaaaa caagtaattg aaaagtgtat actttatatt gccgatacat ctccccgaca
     2701 tgctataata gccgcggttt tcatcgtaaa gatgttttat aaagagcgag gggaaagaat
     2761 ggccagaata gagtacgtaa acattgaaga tgcgttggga atggctcttt cgcacgacat
     2821 gacccagatc gatgtcaagt ccggttacaa gggcgcccgc ttcaagagag gacacattgt
     2881 caaacctgaa gatcttccgg tgctcaggag catggggaag aattccattt cggtcctcta
     2941 cctggaggaa ggcgacgttc acgaagacga cgccgccctc agacttgcac accgccttgc
     3001 aggcgagggc ctccgtatgg agggtcccga ggagggaaaa gtctcgctct tcgccgaatg
     3061 gaacggactg atcgtgtacg acgaagagtc cattcacaca atcaacgagg atgtcgactg
     3121 gattgccgtc acaacgccga acaaggttcc cgtcaaaaag ggagaacccg ccgccggtat
     3181 ccgcgtcgtt ccgttgacga tgcgcgaaga gcagatccgc aaggcggaag cggctgcgct
     3241 tccgctcaca gtctttccgt tccaccctct gaaaacagcg cttgtcacca caggacgcga
     3301 gatcgtcgaa gggcgcatcc gcgacgcttt cgcgccgaag ctggtgcgca agctggcgtt
     3361 ctacggatcg accctgatgg aacaggtcat tacaggcgac gaaaaggacg aaatcgcccg
     3421 tgccattcag tccttcatcg acaagggggc cgagctgatc
//
LOCUS       DAOSWW010000177         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19447_length_3454_cov_3.906737, whole genome shotgun sequence.
ACCESSION   DAOSWW010000177 DAOSWW010000000
VERSION     DAOSWW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19447_length_3454_cov_3.906737"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PK442_04805"
     CDS             complement(<1..649)
                     /locus_tag="PK442_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO86999.1"
                     /translation="MRFLSDTPLFDLHNHTLASGHAYGTIRESAEAAADRGLAVLGFS
                     EHAWTVTGAPSRSYFLNFKEIPSRICGVRIVNGIEANILNESGEIDVDEQLGSRLDFV
                     IASLHPSPYYSGPIAKKEIIRAYIETMRRNPFVRIIGHPEDGRFPVDYRSLAEAASDR
                     GVALELNNSGLREKHGRQNGAANAEELLEECACCGAFVVMGSDSHWGGDAGALANC"
     gene            829..2427
                     /locus_tag="PK442_04810"
     CDS             829..2427
                     /locus_tag="PK442_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOO87000.1"
                     /translation="MAGRIFLTGDVHGDVTSARLGKRLFPEGEGLSKEDFLVVLGDFG
                     LFWHTPRTPEERRCLRSLADRPWTTLFIDGNHENFDLLDALPTEERWGAPVGVAAPGV
                     YHLRRGFVYDVAGLSCFVFGGGRSVDKSVRTPGIDWWERENPGPEERTLGLENLERHG
                     WKVDLVWTHVAPTRACDRLLSDHYAFAHTGRGTAHDPLSDYLDDVAERLSFKLWSFAH
                     YHVSARPFFAGSSGLFTAEYETFREIPIRSGPLPEPKEESAANAEGMDMQLFFFTNKG
                     NVRDANQDALLAGERLVAYEPGKPSHCMERVEAVRSTGNRVLLAVIDGMGGYAGGELA
                     SRIVAESLLDRFPEVISAASAEAAKEYVVRALGTAAELMNELSAEYESLESMGATLAG
                     LVLGKERALLFNVGDCRVYRLRGGVLERVSRDHSEVQNLVDAGIIKEEEMRFHPRKSI
                     VTSAIQAGVGLASTPLFFKEFVPREGDAFFLCSDGVWEALSREEIEKCLLMPDEKAGC
                     EELRERLLDTECRDNVSFVCMRMN"
     gene            complement(3273..>3454)
                     /locus_tag="PK442_04815"
     CDS             complement(3273..>3454)
                     /locus_tag="PK442_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87001.1"
                     /translation="FAEKRYYLRPSTRVDGEKYLRKHFPQNHIIIIDLSKKLFLSNPS
                     PLIALQNVLRPLKRA"
BASE COUNT          744 a          898 c         1063 g          749 t
ORIGIN      
        1 cgcagtttgc gagcgctccc gcgtcgcctc cccagtgcga atcgctcccc atgactacga
       61 acgcgccgca gcacgcacac tcttcaagaa gttcctcagc attggcggcg ccgttttgcc
      121 gtccgtgttt ctcgcgaaga ccggagttgt tcagttcaag agcgacgcct cggtccgaag
      181 ctgcttcggc gagcgaccgg taatcgaccg ggaaacgtcc gtcctccggg tgtccgataa
      241 tcctgacgaa ggggttgcgg cgcatcgttt cgatgtacgc ccggattatc tcctttttcg
      301 caatcggccc cgaatagtaa ggagacgggt ggagcgacgc gataacgaaa tcaaggcgcg
      361 atccaagctg ctcatcgacg tcgatctctc cgctttcgtt caaaatgttg gcttcgattc
      421 cgttcacgat gcggacaccg cagatccggg aaggaatctc cttgaagttg aggaagtacg
      481 agcgggaagg agcgccagtg acagtccacg catgttcgga gaaaccgagc acggcgagtc
      541 cccggtcagc ggctgcttcg gcggactcgc ggattgttcc gtacgcgtgg ccgctggcca
      601 gagtatggtt gtgcaggtcg aagaggggcg tatccgagag aaatcgcatc ggtttcaaac
      661 caacctttcc ggcgattgaa ggccgcatgc gccgaggtgc gcggcggccg tttccttgat
      721 ctgtccctca ttctaacaac aggttcgttc aggtacaagg tttcattagc gttctgaacg
      781 tgcgacaata gttgtgatcc tgcaagaacg gaaaaggagg cggcgaggat ggccggacgg
      841 atatttctga ccggagacgt gcacggggac gtgacttcgg cgaggctcgg caaacggctc
      901 tttcccgagg gggaggggct gagcaaagag gattttttag tcgtgctggg agacttcgga
      961 ctcttctggc acacccctcg gacacccgag gagcggcgct gcctgagaag ccttgccgac
     1021 agaccgtgga cgacgctctt catcgacggc aaccacgaga acttcgacct gctcgacgct
     1081 ctcccgacgg aggagcgatg gggcgctccg gtcggcgtcg ccgctcccgg cgtctaccat
     1141 ctcagaaggg ggttcgtcta cgacgtggca ggcctctcct gcttcgtctt cggaggaggc
     1201 agaagcgtcg acaagagcgt ccgcactccg ggaatcgact ggtgggaaag agagaacccc
     1261 ggtccggagg agcgaacgct cggattggag aatctcgaac ggcacggatg gaaggtggac
     1321 ctcgtctgga cgcacgtcgc gccgacgagg gcgtgcgaca gactgttgag cgaccactac
     1381 gcctttgcgc acaccggaag gggaaccgcc catgatccgt tatccgacta cctcgacgat
     1441 gtcgccgaaa ggctctcctt caaactctgg agcttcgcgc actaccacgt ctcggcgcgt
     1501 cccttcttcg ccggttcaag cggccttttc accgccgagt acgagacctt ccgtgaaatc
     1561 ccgatccgtt ccggtcccct ccccgaaccg aaggaagaaa gcgccgcgaa cgctgaaggg
     1621 atggatatgc agctgttttt cttcaccaac aagggaaatg tgcgcgacgc caaccaggac
     1681 gctcttctgg ccggagaacg gctggtagcg tacgaacccg ggaagccatc gcactgcatg
     1741 gaacgtgtcg aggcggtccg ttccaccgga aaccgcgttc ttctcgcggt gatcgacggg
     1801 atgggcggat acgccggggg agaactcgcc tcgcggatcg tcgcggaatc ccttctcgac
     1861 cgctttccgg aggttatctc agcagcatca gcggaagctg ccaaagagta cgtcgttcgg
     1921 gcgcttggga cggctgcgga gctgatgaac gaactctcgg cggagtatga gtctctcgag
     1981 tcgatgggag ccacgcttgc cggactggtt ctcggaaagg agcgcgccct tctcttcaac
     2041 gtgggcgact gccgcgtcta tcgccttcgg ggaggcgttc tcgaacgggt gagccgcgac
     2101 cactccgagg tacagaacct tgtggacgcg gggataatca aagaagaaga gatgcggttc
     2161 catccccgga agagtatcgt gacctctgcc atccaggccg gtgtcggtct cgcctccacc
     2221 cctctttttt tcaaagagtt cgttcctcgt gagggggacg ctttctttct gtgcagcgac
     2281 ggcgtctggg aggcgttgtc gcgggaagag atagagaagt gcctcctcat gcccgatgaa
     2341 aaagctgggt gcgaggaact acgggaaaga ctgctggata cggagtgccg ggacaacgtc
     2401 agcttcgtct gtatgcggat gaactaataa aaaaagcgaa aggattttgc ggcacaaaga
     2461 gcctgatgtg aaccgcctaa agtactttga ggaagagaaa ctcaatggaa gacatggccg
     2521 gcctaagcca cggcgtttgg gaatgcaaat acaaataggt atggatagcg aaatttagag
     2581 ggatgtttgt tcggtcagat ccggaaggag ttcggtgaag ttttcatcgg ttgacggagc
     2641 agaaaggtgc tcggattcac gaaggtcgtc ttgcatcgga ccgcgtgcac atgtacgtat
     2701 cgatactaac gaagtattca gcggcgaagt tggtgggttt tgcgaagggc aggggagcgg
     2761 tgcatatagc gaggacgtat ggaggcaaag ccggaatttt tccggtggta attttctggc
     2821 acgaggatct ttagtagcgg cggtggggcg tgacgaacag gtgatacgag agcatattcg
     2881 gaggcagaag gaaaaggaaa aaaatttttg gatcagtcga ggctgtttga ggagccgtct
     2941 ttaggcggct cctcatttgt cacccatctt gggtgccaaa tgattctcaa cagttttgag
     3001 cggttctaaa aacccctgct tttggtgggg gctgtgacta aaaagttttt tccgtgcggg
     3061 atatgtggga ggtttctctc ggtctttttt cctcaagggg gacatcgtcc actgacgtgg
     3121 acgatgtccc aatccagtcc gctttgcgga ctggatgcac aacggacccg atatcgatcc
     3181 actgtctttt tacatcctcg cctcaattct ttacggacga acggtgcaat aacaccgttc
     3241 tattacgact gtttacgatt attccgtgcg tcctacgccc tcttaagagg gcgtaggacg
     3301 ttttgcaacg cgatcagggg agagggattt gagagaaaaa gttttttgga aaggtcgatt
     3361 atgataatat gattttgtgg gaagtgtttc cgtaaatatt tttcaccgtc cacgcgcgtg
     3421 gacggcctca ggtaatatct tttttctgcg aaga
//
LOCUS       DAOSWW010000178         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19448_length_3454_cov_3.011474, whole genome shotgun sequence.
ACCESSION   DAOSWW010000178 DAOSWW010000000
VERSION     DAOSWW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19448_length_3454_cov_3.011474"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PK442_04820"
     CDS             complement(<1..726)
                     /locus_tag="PK442_04820"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOO87002.1"
                     /translation="MNTVRGIFAPIATAFDASGEVNYDVFAENVRSFGATKLAGLVVL
                     GSNGEFTLLSHDEKVKLVETARKHLPSDKKIIAGTGCESLKETVELTKQCAAAGADVA
                     LVVTPNYYKKDMNEAALEKFYTMLADASPVPVMIYNMPGNSGVNVPSPLTLKLAAHPN
                     ITGIKDSGGNIVQISEVLAKAPSDFSVFAGSGSYLLATLVLGGVGGTLAVANVVPDYC
                     AEIQERFEKGDLDGARKMQLALLP"
     gene            857..1294
                     /locus_tag="PK442_04825"
     CDS             857..1294
                     /locus_tag="PK442_04825"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO87003.1"
                     /translation="MTDFFETLSPAEKDTGGSIPEETVRLIAERCECDERVLAVYLLG
                     SAARGTMRPDSDIDIGVLPAPGEFFSALELAEISAEISFETGYVVDAGLVDGTDLVYA
                     NQALDGVRLFVREGTTTELREAALRAMYLRFCEDRKEVVDAYS"
     gene            1281..1697
                     /locus_tag="PK442_04830"
     CDS             1281..1697
                     /locus_tag="PK442_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOO87004.1"
                     /translation="MPTVDDVCRNKGSIIERTLRRMKEEYAADPELKSFTHIDAMTLN
                     IERACQAAIDAAMHIAARDHLGMPRNSADVFRLLHRAGRLSETTARAMVAMTGFRNVA
                     IHEYRELDMSVLRAIAADRWTSLVDFCREIGLTIRP"
     gene            1711..>3454
                     /locus_tag="PK442_04835"
     CDS             1711..>3454
                     /locus_tag="PK442_04835"
                     /inference="COORDINATES: protein motif:HMM:NF023835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase-associated domain-containing
                     protein"
                     /protein_id="HOO87005.1"
                     /translation="MKALHISLLNGNPVVWCEGGSLFDALEAIEFSLSRSAPLSAMTL
                     WLPGRKGVPFPSSSLLGEAEGRGKVALVPFRTQGRSPSTFAEAAALAYRFEGSSPRGS
                     GVLFGSSVRWFAAAMRAVRLLTAGESVLPAAARTEGVWEARWIPAPDDSAQRLLEELA
                     ASMPHVCRSIASDEQGPPDHSPRRVLAAFVGRFVDLAMRGEERRKEEAPKGRKKKTAS
                     SDAGSLHDAWREALSGADARILWSDENELSEFARALSAWRRPVEFASKAAYRFCFRLE
                     EPEEGDDWRLVYLVQPKADPSLLLPLDALWEQTPPPKKSRKKGPAPSPLPAARDVSAE
                     FLLTMLGQAAALSPRVAQSLKQPAPSSIRMSSEEAFSFLGGEAQTLADAGFQMMLPRW
                     FVKGPGERIKVKAVVKAPTMQSASGMSLESVMEFDYALSLAGETLAPEELEMLVMLKG
                     DFVRFRGQWMKVDRESIASGLRLFSQKKETRATGRDLLMKAVGGEGALSGVSVDSAEL
                     HGWPAELLAKLVEKRSDDLPSPEGLRGVLRPYQQRGYSWLAFLKEWGLGACLADDMGL
                     GKSVQALALVQRERE"
BASE COUNT          623 a         1072 c         1102 g          657 t
ORIGIN      
        1 cggcagcaga gcgagctgca tcttccgcgc tccgtcgagg tcgcccttct cgaagcgttc
       61 ctgaatctcg gcgcagtagt cggggacgac gttggccacg gcaagggtgc cgccgacgcc
      121 tccgagaacg agggtggcca gaaggtagct ccccgacccg gcaaagaccg aaaagtccga
      181 gggagccttg gccagcacct cggagatctg cacgatgttg ccgccgctgt ccttgatgcc
      241 ggtgatattc ggatgagcgg caagcttgag cgtcagcggg gaggggacgt tcacgccgga
      301 gttgccgggc atgttgtaga tcatcacggg gaccggggag gcgtcggcga gcatcgtgta
      361 gaacttctcc agcgcggcct cgttcatatc ttttttatag tagttcgggg tgacgaccag
      421 cgccacatcc gcgcccgcgg ccgcacactg tttcgtcagt tcgacggtct ccttcagcga
      481 ctcgcacccc gttccggcga tgatcttctt gtcggagggg aggtgttttc gagccgtctc
      541 caccagcttc accttttcgt cgtgcgagag cagcgtaaac tcgccgttgc tccccagaac
      601 cacaagcccc gcaagcttcg tcgcgccgaa agagcgcacg ttctccgcga aaacgtcgta
      661 gttcacctct cccgaagcgt cgaacgccgt agcgatgggg gcgaaaattc cgcgaaccgt
      721 attcataccc gatctctcct ttagaatgca ttatgtttct tatatagtac ttcaaaaaac
      781 cgtttccgtg aagaggctct cgattttgtg cgggccgatg cgttacaatg ttggtgaagc
      841 aaaaggggga agcggtatga cggacttttt tgaaactctt tctccggcgg agaaagatac
      901 agggggctcg atccccgaag agacggtccg gttgatagcg gagcgttgcg aatgcgacga
      961 gcgcgtgctt gcggtctatc tgctcggcag cgccgctcgg gggacgatgc gccccgacag
     1021 cgatatcgac atcggagttc ttcccgcccc cggagagttc ttttccgctc ttgaactcgc
     1081 cgaaatctcc gccgaaatct ccttcgaaac gggatacgtt gtcgacgccg ggctcgtcga
     1141 cgggaccgat cttgtctacg ccaaccaagc gctggacggg gtgcgtcttt tcgtccggga
     1201 agggacgacg acggaactgc gcgaagcggc gctgcgcgcc atgtaccttc gcttttgcga
     1261 ggacagaaag gaggtcgtcg atgcctacag ttgacgatgt atgcaggaac aagggctcca
     1321 tcatcgagcg gacgctgcgc cggatgaagg aagaatatgc cgccgacccg gagctgaaga
     1381 gcttcacgca catcgacgcc atgacgctga acatagaacg cgcctgccaa gccgccatcg
     1441 acgcggcaat gcacattgcg gcgcgggatc acctcggcat gccccgcaac agcgcggacg
     1501 tcttccgtct gctccatcgg gccggacgcc tctccgagac gacggcgcgg gcgatggttg
     1561 cgatgacggg gttccgcaac gtcgcaatcc atgaataccg cgaacttgat atgtcggtgc
     1621 ttcgcgccat cgcggccgac cggtggacgt cccttgtgga cttctgccgg gagatcggcc
     1681 tgaccatccg cccttgagga gctgaacccc ttgaaagcac tgcacatctc gctgttgaac
     1741 ggaaaccctg tcgtctggtg cgaaggtgga tctcttttcg acgcgcttga agccatcgaa
     1801 ttttcccttt cccgctcggc ccccctgagc gccatgaccc tttggctgcc ggggagaaag
     1861 ggcgtcccgt tcccctcgtc atctctgctg ggagaggcgg aagggcgcgg aaaggtcgcg
     1921 ctcgtcccct tccgtacgca ggggcggtct ccgtctacgt tcgcggaggc ggcagcgctc
     1981 gcgtaccggt tcgagggaag ctctccgcga ggctcaggcg ttctcttcgg ctcgtccgtc
     2041 aggtggttcg ccgcggcgat gcgggccgtc cggctgctca cggccggaga atccgtcctc
     2101 cctgcggcgg cgcggacgga gggcgtatgg gaagcccgct ggattccggc gcccgacgac
     2161 tccgcccagc gtctgctcga agagctggcg gcctcgatgc cccatgtctg ccggagcatc
     2221 gcttccgatg agcaaggccc gcccgaccac tctccccggc gcgttctggc ggcctttgtg
     2281 gggcgcttcg tcgatctggc gatgcgcggc gaagaaagac ggaaggaaga ggcgccgaag
     2341 ggcagaaaga agaaaacagc gtcgtcggac gccggaagcc tccacgacgc gtggagagag
     2401 gctctttccg gagcggatgc ccggatcctc tggtccgatg aaaacgaact ctcggagttc
     2461 gcacgcgcgc tttcggcatg gcgccgccct gtggaattcg cctcgaaggc cgcgtaccgc
     2521 ttctgtttcc gcctcgaaga gcccgaagag ggcgacgact ggcggctcgt ctacctggtc
     2581 cagccgaagg ccgacccgag ccttcttctt cctctcgatg cgctctggga gcagactccg
     2641 cctccgaaaa aaagccgcaa gaaggggccc gctccctcgc cgcttcccgc ggcccgagac
     2701 gtctctgcgg agtttctcct cacgatgctc ggacaggctg cggctctttc tcctcgggtc
     2761 gcgcagagcc tgaagcaacc cgctccttct tcgatccgca tgtcctccga ggaggcgttt
     2821 tccttcctcg gaggggaggc gcaaacgctt gccgacgcgg ggttccagat gatgcttccc
     2881 agatggttcg taaaaggccc cggagaacgc atcaaggtaa aggcggtggt gaaagcgccc
     2941 accatgcaga gcgcatcggg gatgtcgctc gaaagcgtga tggagttcga ctacgcgctc
     3001 tcgctcgccg gcgagacgct cgcgcccgaa gagctggaga tgctcgtaat gctcaaggga
     3061 gatttcgtcc gtttccgcgg tcaatggatg aaggtggacc gcgagagcat tgcttccggg
     3121 ctccgtctct tttctcaaaa gaaagaaacg cgggctaccg gccgcgatct gctcatgaag
     3181 gcggtgggcg gcgagggggc gctctccggc gtctccgttg attccgcgga gctgcacggc
     3241 tggcctgcgg agctgctcgc gaagctcgta gagaagagga gcgacgatct cccctctccg
     3301 gaaggactac gcggcgtcct tcgcccctac cagcaacggg ggtactcgtg gctcgccttc
     3361 ctgaaggagt gggggctcgg cgcctgtctc gccgacgata tggggctcgg gaaaagcgta
     3421 caagccctgg ccctcgtcca gagggagcgc gaac
//
LOCUS       DAOSWW010000179         3445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19545_length_3445_cov_3.489086, whole genome shotgun sequence.
ACCESSION   DAOSWW010000179 DAOSWW010000000
VERSION     DAOSWW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19545_length_3445_cov_3.489086"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..652)
                     /locus_tag="PK442_04840"
     CDS             complement(110..652)
                     /locus_tag="PK442_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO87006.1"
                     /translation="MEKKITIAGFGGQGVMAIGQMLGYASCKAGGNALFLPKYGPEQR
                     GGTANCTVTLADVEIGAPESRYVDTLIALNQPSLERFLGAVRPGGLVLLNSSLCRWTG
                     GREDVKLAEIPADDIAREIGSPKVANIVVIGAYIGLSGVLTERQMLDAIEEKLGKRPE
                     LLEMNRLALKRGMESATPRT"
     gene            complement(655..1395)
                     /locus_tag="PK442_04845"
     CDS             complement(655..1395)
                     /locus_tag="PK442_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO87007.1"
                     /translation="MDALYERPKSLTNRATAYCPGCLHGVATRLIAELIDELDARADT
                     VAVLPVGCGTLGMFHFNTDIVVSPHGRTPAVATGVKRSAPEKLVFSYQGDGDLAAIGL
                     AEIMHAANRGENFTVIFINNGTYGMTGGQMAPTTLVGQKSTTTPEGRNPAETGYPMMM
                     CEILSQLRAPRYIARFSLDCPANVIQAKKGLRKSFELQRAGKGFTFVELLSNCPTNWG
                     LSPLECLEWMRENTMKAFPLGVFRDRED"
     gene            complement(1397..2473)
                     /gene="vorB"
                     /locus_tag="PK442_04850"
     CDS             complement(1397..2473)
                     /gene="vorB"
                     /locus_tag="PK442_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOO87008.1"
                     /translation="MEKKFLKGNEAVAEAAILAGCRFFAGYPITPQNEIPEYMARRMP
                     QVGGVFLQGESETASVCMVYGAAAMGKLAMTSSSGPGISLKAEGISTLAGAQLPAVIV
                     NVMRGGPGLGTIQAAQSDYLHATKAMGHGGFRVLVLAPSTVQEAIDLTYKAFGLAVKY
                     QNPAVVLMDGWIGSMMEPVTLPEAKEPVKNAYTGWGTRYYNPSGSDVDVVTSCLLEPD
                     RQELLNIHFGEMYETWARDEVMVEEYMLGDAEYVIVAYGTAARAARTAIGSLREKGVK
                     AGMLRPISLYPFPYDALKRLDKSRVRCILDTEMTLPVQMKDDIRLGVTDAIPVHSCGR
                     SGGVVLTNEDIEDALLRIIEEEGK"
     gene            complement(2525..2725)
                     /locus_tag="PK442_04855"
     CDS             complement(2525..2725)
                     /locus_tag="PK442_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOO87009.1"
                     /translation="MKITINAEFCKSCGLCIDVCKRDVLEIGPETNSKGYRAVVAAHP
                     EHCVGCSVCAVMCPEGAIALKK"
     gene            complement(2728..3378)
                     /locus_tag="PK442_04860"
     CDS             complement(2728..3378)
                     /locus_tag="PK442_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87010.1"
                     /translation="MRNAVFLGPSGGGKSEISINMALRAAAEDGPAVHFFDMDQTKPL
                     FRSRACRDLLERSGVVFHSGAEFLDSPVIPDGVADFLRDPACRCILDVGGNPAGARPL
                     GQLAALREADAAAFYVINVYRPFSDEQSLREDMERIVASARIPAPCVVSNPNLGRETT
                     LDDVLEGHRRLLPLLERLDAPLDRLCVRLPLLESVRPLVDCPVAGIALHLDGREVK"
BASE COUNT          586 a         1115 c         1116 g          628 t
ORIGIN      
        1 aagcgttgcg ctggtgtacc cgcaacgcaa catcatactg catgccgcgg aaaagagcgt
       61 cccggcggcg cggcgaatcg caagcccgtc cacggggacg gacgttgtcc tacgtcctcg
      121 gcgtcgccga ctccattccg cgcttcagcg cgaggcggtt catctcgagc agttcggggc
      181 gcttccccag cttctcctcg atggcgtcga gcatctgccg ctcggtgagg acgccgctca
      241 gtccgatgta cgcgccgatg acgacaatgt tcgccacctt cggcgagccg atctcgcgcg
      301 cgatgtcgtc ggccggaatc tcggcgagct tcacgtcctc ccgccctccg gtccagcggc
      361 agagcgagct gttgagcagg acgagccccc ccggacggac tgcgccgagg aaacgctcca
      421 gcgacggctg gttcagcgcg atcagcgtgt cgacgtagcg cgactccggc gcgccgatct
      481 caacgtcggc gagagtcact gtgcagttgg ccgttccgcc gcgctgctcg gggccgtatt
      541 tcggcaggaa gagcgcgttg ccgcccgctt tgcaggaggc gtaccccagc atctggccga
      601 tggccatcac tccctgaccg ccgaagcccg cgatggtgat cttcttctcc atgatcagtc
      661 ctccctgtcc ctgaagacgc caagcgggaa ggccttcatg gtgttctccc gcatccattc
      721 caggcactcc agcggcgaaa gtccccagtt cgtcggacag ttcgaaagca gctcgacgaa
      781 ggtgaagccc ttccccgcgc gctgcagctc gaaggacttc ctgagcccct tcttcgcctg
      841 aatgacgttc gccgggcagt cgagcgagaa acgcgcgatg tagcgcggcg cgcggagctg
      901 cgacaggatt tcgcacatca tcatcgggta gccagtctcg gcggggttgc gcccctccgg
      961 cgtcgtggtc gacttctggc cgacgagcgt cgtcggggcc atctgaccgc ccgtcatgcc
     1021 gtaggtgccg ttgttgatga agatcacggt gaagttctcc ccccggttcg cggcgtgcat
     1081 gatctccgcg aggccgatgg cggcgaggtc gccgtccccc tggtaggaaa agacgagttt
     1141 ctccggagcg gagcgcttca cgcccgtggc tacggcggga gtacgtccgt gaggcgagac
     1201 gacgatatcc gtgttgaagt ggaacatccc cagcgtgccg cagccgacgg gaagcaccgc
     1261 gacggtgtcg gcgcgcgcgt ccagctcgtc gataagttcg gcgatcaggc gggtggccac
     1321 gccgtgcaga cagccggggc agtaggcggt cgctctgttg gtcagcgact tcgggcgttc
     1381 atagagcgcg tccattttac ttcccctcct cttcgatgat ccgcagcagc gcgtcctcga
     1441 tgtcctcgtt cgtcagcacc acgcctccgg agcgcccgca ggagtgcacc ggaatggcgt
     1501 cggtcacgcc gaggcggatg tcgtccttca tctggaccgg cagggtcatc tccgtatcca
     1561 gaatgcagcg gacgcggctc ttgtccaggc gcttcagggc gtcgtacgga aaggggtaga
     1621 gggagatcgg gcgaagcatc cccgccttga cgcccttttc gcgcagagag ccgatcgcgg
     1681 tgcgggccgc gcgggccgca gtcccgtagg cgacgatgac gtactccgcg tcgccgagca
     1741 tgtactcctc gaccatgact tcgtcgcgcg cccaggtttc gtacatctcg ccgaagtgga
     1801 tattcagcag ctcctggcgg tcgggctcca gcaggcagga ggtgaccacg tccacgtcgc
     1861 ttcccgaggg gttgtagtag cgcgtccccc accccgtgta cgcgttcttc acgggctcct
     1921 tcgcctcggg gagcgtcacg ggttccatca tcgagccgat ccagccgtcc atcaggacga
     1981 ctgcggggtt ctggtacttc acagcgaggc cgaaggcttt ataggtaagg tctatagcct
     2041 cctgcaccgt actcggcgcg aggacgagca cgcggaatcc gccgtgtccc atcgccttcg
     2101 tagcgtggag gtagtcggac tgcgccgcct ggatggtgcc cagtccgggc cctccgcgca
     2161 tcacgttgac gatgacggcg ggcagctgcg cccctgcgag cgtggagatc ccctcggcct
     2221 tcaggctgat ccccggtccc gaggaggagg tcatggcgag cttccccatg gccgccgcgc
     2281 cgtagaccat gcagacggag gctgtttcgc tctctccctg caggaagacc ccgccgacct
     2341 gcggcatccg ccgcgccatg tactccggga tctcgttctg gggcgtgatc gggtagcccg
     2401 cgaagaagcg gcacccggcg aggattgcag cctcggcgac ggcttcgttt cccttcaaaa
     2461 atttcttttc catagtctca atcctttcgt gtggcggagt gcggggagcg tccttccgtt
     2521 cccgtcattt cttgagcgca atagccccct cgggacacat gaccgcgcat acggagcagc
     2581 cgacgcagtg ttcgggatgc gccgcgacga ccgcccggta tcctttcgaa ttggtctccg
     2641 gcccgatttc cagtacgtcc cgtttgcaga cgtcgataca gaggccgcag gacttgcaga
     2701 actcggcgtt gatggtgatt ttcaccgtta tttcacctcc cttccgtcaa ggtggagggc
     2761 gatccccgcg acggggcagt cgacgagagg acgcaccgat tccagaagag ggagccggac
     2821 gcagagacgg tcgagcggcg catcgagccg ctccagcaga gggagcagcc tgcggtgtcc
     2881 ttcaagaacg tcgtcgagcg tcgtctcccg tcccaggttc ggattgctca ccacgcacgg
     2941 agcggggatg cgggccgatg cgacgatgcg ttccatgtcc tcccggagac tctgttcgtc
     3001 ggagaacggg cggtagacgt tgatcacgta gaaagcggcc gcgtcggctt cacgcagggc
     3061 ggcgagctgt ccgagcggac gagctcccgc agggttgccc cccacgtcga ggatgcagcg
     3121 gcaggcgggg tcgcgcagaa aatccgcgac gccgtccgga atgacgggag agtcgagaaa
     3181 ttcggccccc gaatggaaga cgacgccgga gcgctccaga aggtcgcggc aggcccggga
     3241 gcggaagagc ggcttcgtct ggtccatgtc gaagaagtga acggcaggcc cgtcctctgc
     3301 ggctgcgcgc agcgccatgt tgatggagat ctccgacttg cctccgccgg aagggccaag
     3361 aaagaccgcg ttgcgcacgc catcgcctcc tagataaaaa tcaaaatatt ttatatttag
     3421 gcataaactg tgaaatcaag acggt
//
LOCUS       DAOSWW010000180         3444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19552_length_3444_cov_3.858365, whole genome shotgun sequence.
ACCESSION   DAOSWW010000180 DAOSWW010000000
VERSION     DAOSWW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19552_length_3444_cov_3.858365"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..522
                     /gene="rbfA"
                     /locus_tag="PK442_04865"
     CDS             109..522
                     /gene="rbfA"
                     /locus_tag="PK442_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOO87011.1"
                     /translation="MTTFRMQRINKQIQREVSLLLELRIKNETAKKAIITDVDCSRDL
                     EHAKVYFTTVDRSEREEVGKALGTVAGALRSQLGKLLTIRQIPALSFFFDTSEEYGRS
                     IDVLLDSLKTEDSVIERDEDDETEDVSDTDELPEA"
     gene            539..1549
                     /locus_tag="PK442_04870"
     CDS             539..1549
                     /locus_tag="PK442_04870"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOO87012.1"
                     /translation="MNDSYASSDGFPALIRILSEASEWIVLSHVKPDGDALGSASAFA
                     SVGRKLGKTVVWGGPDEYPSRYLFLEGSGEFRPNLKLSSLALTEGSVVLALDTSTRAR
                     SVEDLDALPENVPLLNVDHHADNELFGTVNYVDPSSSSTGEIAWLLLREWGIPLEIDV
                     LEAVYTALATDCGNFAFSCTTPRTHRVAAELLDGGVSPAKLDERIRCNASIPGLRLRA
                     VALSRAFVAGGFAAFTWLRNDDFLRNGSERSATEFLVNDLLTLKDVSFAAFFVEDEEC
                     VRVSLRSRGVVLAAEIARVFGGGGHPQAAGCKLPLPLSDAVESVRSLVEERYAERIAP
                     AE"
     gene            1521..2435
                     /gene="truB"
                     /locus_tag="PK442_04875"
     CDS             1521..2435
                     /gene="truB"
                     /locus_tag="PK442_04875"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOO87013.1"
                     /translation="MPSGLLLLNKPAGVRSAVCVAIAKRRLNREFKIGHGGTLDSTAE
                     GLLVLLIGGATRTSDLVMSLPKLYEAAFTLGEERSTDDYSGDTVFEGRVPNDARARIE
                     AALPSFLGVRLQLPPGISAVRVGGRRAHAVVRSGEELSLRSRPVFVRSISLLPGEEMK
                     NVFTIRVLCGKGTYIRSIVRDIGRMLGCGAYVLRLIRKQSGLLSLGQALPFEEFQNGD
                     ADLRSHIFPLARFAENFYCYEADEERTARLRDGLAVPLSSLRFSHEGILSPDQGAAVL
                     GKTLFCYGRVLKEGLFKPKINIDLEGTE"
     gene            2432..3373
                     /gene="ribF"
                     /locus_tag="PK442_04880"
     CDS             2432..3373
                     /gene="ribF"
                     /locus_tag="PK442_04880"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOO87014.1"
                     /translation="MIVVLGAFDGFHRGHALLLDRARELSGRHSRSAANGEWAVVTFS
                     PHPDSVVRGGMFSLFSEEERDLLVRFFNVPHIEKIPFDETLSRTSAEGFIALLESLLP
                     VTGVIVGEDFRFGRDREGDAAYLREVARRKGWLFDKLDSFVLEGAKVGSSSIKKHVIS
                     GDVDRARNLLGYPFFFRGRVVSGDGRGRTLGFPTANIEYPEKKILPQGGVYAASVLIG
                     NEWWPGALNIGFNPTFLSSVENPRFETYVLDLNESLYGRTITVFLESLLRPEIKFESS
                     DALLHQMQLDVLRVRDCFREMHARHGDVYCHLSKAFS"
BASE COUNT          722 a          863 c         1000 g          859 t
ORIGIN      
        1 gggttgctat ggcggaggag cggctccagg cggtgtactc ctttttaacc aacgaggagg
       61 agaccgggga gttctcgatc ctatatcact ggcgcgaggt aacacgttat gacgacattt
      121 cgaatgcaga gaatcaacaa gcagatccag agagaagttt cgcttctgct ggagctgcgc
      181 ataaagaacg aaacggcaaa gaaagcgatc atcactgatg tcgactgttc tcgtgatctt
      241 gaacatgcaa aagtgtactt tacgacagtc gaccgttccg agcgggaaga agtagggaag
      301 gcgctcggaa cggtcgcagg ggctctgcgg agtcagctcg gaaaactttt gacgatacgg
      361 cagattcccg ccctttcctt ttttttcgat accagcgagg agtacgggcg cagtatcgat
      421 gtgctgctgg attcgttgaa gaccgaggat tccgttattg agagggatga agacgacgag
      481 accgaagatg tctccgacac ggatgaactg ccggaggcgt agtgacgggg aggatgaaat
      541 gaacgattcg tatgcttcgt cggacggctt ccccgcgctc attcgcattc tttctgaagc
      601 ctcggaatgg attgttctct cacatgttaa acccgacggc gatgcgctcg gttccgcgag
      661 cgctttcgct tcggttgggc ggaaactcgg aaagactgtc gtctggggcg gcccggacga
      721 gtacccttcc cgttacctgt ttcttgaagg ttccggggag ttccgtccga acctgaagct
      781 ttcctcgctg gcactgacgg agggaagcgt ggttctcgcg ttggatacgt cgacccgtgc
      841 gcgttctgtg gaggatcttg acgctcttcc cgaaaacgtg cccctgctca acgtggatca
      901 ccatgcggac aatgaattat tcgggactgt caactatgtg gatccctcct cctcttcgac
      961 cggcgagata gcctggctgc tccttcgcga gtgggggata cccttggaaa tcgatgtgct
     1021 ggaagctgtg tacactgctc tcgcgactga ctgcgggaac tttgcgtttt cgtgtactac
     1081 gccgcggacc cacagggttg cagcggaact tcttgacgga ggggtttctc cggcaaagtt
     1141 ggatgagcga atccggtgca atgcatccat tcccggtctt cgtttaaggg cggtggcatt
     1201 gtccagagcc ttcgttgcag gcggttttgc ggcgtttaca tggttgagga acgatgattt
     1261 tctccgtaac ggcagcgaac gctctgcaac tgagttcctg gtaaacgatc tcctcacgct
     1321 caaggatgta tcgtttgccg ctttctttgt ggaggacgag gagtgcgtcc gcgtatcgct
     1381 ccggtcgaga ggcgtcgttc tggcggctga aatagcgcgc gtattcggtg gaggagggca
     1441 tccccaggcc gcggggtgca agcttcctct tccattatcg gacgctgtgg agagtgtacg
     1501 ctctctggtg gaggaacggt atgccgagcg gattgctcct gctgaataaa cctgccggcg
     1561 tacgaagcgc cgtctgtgta gccatcgcga aaaggcgcct gaacagagag ttcaagatag
     1621 gtcacggcgg cacccttgat tctacagctg aaggcctgct tgtgctcctg attggaggag
     1681 ctacccggac gagcgatctt gtgatgtccc tgccaaaact ctacgaggcc gccttcacgc
     1741 ttggagagga gcgctcaacc gacgactact ccggcgatac tgttttcgaa gggcgcgtcc
     1801 cgaatgatgc acgggctcgt atagaggcgg cgctcccgtc atttctcgga gttcggctcc
     1861 aacttcctcc gggaatatca gccgttcggg taggaggaag acgggctcat gctgtcgtgc
     1921 gttcgggaga agagctctct cttcgcagcc gacctgtttt tgtacgttcc atttcgctcc
     1981 ttcccggcga agagatgaaa aatgtgttca caatacgcgt tctctgcggg aaaggaacat
     2041 atattcgcag tatcgttcgc gatataggaa gaatgctcgg gtgcggggca tacgtcctcc
     2101 ggcttatccg aaaacagtcc ggattactct ctttggggca ggctctccct tttgaagagt
     2161 tccaaaacgg ggatgccgat cttcgatcac acattttccc gctcgctcgt tttgcggaga
     2221 atttttactg ctatgaagcc gatgaagaac gaacagcccg tctgcgcgac ggcctcgcag
     2281 tgccgctttc ctcccttcgt ttttcgcatg aaggtatact ttctcctgac cagggagcgg
     2341 cggtcctggg aaaaactctt ttttgctacg gacgagttct taaggaagga ttgttcaagc
     2401 cgaaaatcaa tattgatctg gagggaaccg aatgattgtc gtgcttggag ctttcgacgg
     2461 attccacaga ggacacgctc ttcttctcga tagagccaga gagctttccg ggcgacatag
     2521 ccgttctgct gcaaacggag agtgggcagt agttactttt tctccgcacc cggattcggt
     2581 tgtccggggg ggaatgtttt ccctcttttc cgaggaggag cgcgatcttc tcgttcgctt
     2641 cttcaacgtt ccgcatattg aaaaaattcc atttgacgag acgctctcac ggacctcggc
     2701 ggagggattc attgccctgt tggagtcgct tcttcctgtg actggagtta tagtgggaga
     2761 agattttcgc ttcgggcgcg accgggaggg ggacgcggca tacttacggg aggtggccag
     2821 acggaaggga tggctctttg acaagttgga ttcctttgtt cttgaaggag caaaagtcgg
     2881 tagcagcagt ataaaaaagc atgtcatttc aggagacgtc gatcgtgcca gaaatctgct
     2941 tggatatcct ttctttttca ggggaagggt tgtctctgga gatggcagag ggcgtacttt
     3001 aggcttccct accgcaaaca tcgaatatcc cgagaaaaaa attctgcccc aaggaggagt
     3061 ctacgccgcc tcggtactga tcggaaacga atggtggccg ggagccctga atattggttt
     3121 taacccgaca tttctttcct ccgtagaaaa ccctcgtttc gaaacctatg tgctggacct
     3181 gaacgaatcc ctctacggga gaaccataac cgtcttcctt gagtcgcttc tgcgtcccga
     3241 aatcaaattt gaatccagcg acgctcttct tcatcagatg cagctcgatg ttcttcgagt
     3301 gagagactgc ttcagggaaa tgcatgcccg tcacggtgat gtctattgcc atttgtcaaa
     3361 agcattttct tgacgtgtgc gccttgcgac ttgaaaaatc ggggagaggg tggtactatt
     3421 cctcgtgctg gcgattttcc ggca
//
LOCUS       DAOSWW010000181         3438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19612_length_3438_cov_3.231451, whole genome shotgun sequence.
ACCESSION   DAOSWW010000181 DAOSWW010000000
VERSION     DAOSWW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19612_length_3438_cov_3.231451"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PK442_04885"
     CDS             <1..1168
                     /locus_tag="PK442_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87015.1"
                     /translation="YLNSAFESQISTFDATLPAGGSTRTAFAVEEGTELLRVSLVSDG
                     EISPKTLVFSATPPKGKASAALHDKETGKLEILQKKPAAGKWAVTLKNNSKGTRRVRI
                     SAFSRTQSRTLYLGNALTVRGGEANSAVIYASLTGNVTPHTGIGIKGTLRYPSGKNVP
                     LEFQDMGYGPDATQGDGVYSALAENCSEPGNYRVTVAFSNTGQAVETNQRYAFLPPPG
                     IKDGEAYVVAEKGHALPHKLERVVATTFQIEGGKVTDDSRGIIAREKPIDFERVKSYL
                     ERGEKALKRGAYDDAEDYAKSVLRMDPNNAAAYRLIGEAKDARNDFAGAVEPYAKAVE
                     LEPGEPSHYFKLGQALANIRALDEAKKIYSIMQQAFPNNKWTNQLEMAIRFMSR"
     gene            1482..1865
                     /locus_tag="PK442_04890"
     CDS             1482..1865
                     /locus_tag="PK442_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87016.1"
                     /translation="MRTTVANRLLYYRNTVADTGRMNPDIATLRKENLLLLLSREEAM
                     SGVPEDAEAAKAFLERSLKTPREGASGGDDDGRVAGVLCPWIYTPRVVHGAAAEGAPF
                     ALLCVPAILSSEGRLLPSGEPPWIP"
     gene            1941..>3438
                     /locus_tag="PK442_04895"
     CDS             1941..>3438
                     /locus_tag="PK442_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87017.1"
                     /translation="MESGSVETWEELAATCSRYIAAVTGSETPPEIEGYLIDGRAAFF
                     AADTIKATLHLAKLYDALLADKGSFPLLERMIEGANTSEPLLSPEEEIEVSRGHVGCM
                     SPKFPLSPSQRKALHHFMRTGRGEVLALNCHPGTGKTTMLQSVVAQLWVDAALRKTEC
                     PLIVASSTNNQAVTNIIDSFGKVARDENDPLYERWLPGITGFGLQMAREERWKEGFHA
                     YDNPFRNGKPETSGLFEKIERPEWLEEAEALFLEKASERYGPLTNVASASERLHAELW
                     ECAESVGSLLDALLRTAPSSPDAKIGEETRRRLGELEESLESAAKELENGRRLLARAE
                     ETRILWEKHLASESFLVALLSFLPPVRKRKEAADRAFLLERSIACESADREKIREALA
                     RQREEAAKGASEAEARLNERTRALSSLEESWSKAASLASLARINLDAEDVSLNEATER
                     LDTSVRAKAFRVASHYWEAEYLRELRGNIESKYRDSAAPDKRARMYRRF"
BASE COUNT          747 a         1067 c         1055 g          569 t
ORIGIN      
        1 ctatctcaac agcgccttcg aaagccagat atccacgttc gacgcaacgc ttcctgcagg
       61 cggctcgacg cggacggcgt tcgccgtgga ggaaggcacg gaactgctgc gggtctctct
      121 tgtttccgac ggagagatct cgcccaagac gctcgtattc tccgcgactc ccccgaaggg
      181 caaagcttcc gccgcgcttc acgataagga aaccgggaag ctggagatcc ttcagaagaa
      241 acccgcggcc gggaaatggg ccgtcacgct gaagaacaac tcgaaaggaa cgcggcgcgt
      301 aagaatctcc gcattctcac gaacgcaatc cagaacgctc tacctgggga acgccctcac
      361 cgtgagaggc ggggaagcga actccgcagt catatacgca tccctcacgg gaaatgtaac
      421 ccctcatacg ggaatcggca tcaaggggac gcttcggtac ccttcaggga agaatgttcc
      481 tcttgaattt caggatatgg gctacggccc cgatgccaca cagggggacg gcgtctacag
      541 cgctctcgct gagaactgct cggaaccggg gaattacaga gtcacagtcg ctttttccaa
      601 taccgggcag gcggtggaga ccaaccagcg ctacgccttt ctgcctcctc ccggcataaa
      661 ggacggcgag gcgtacgtcg tggccgaaaa aggacacgct ttgcctcaca aactcgaacg
      721 cgtcgtggcg acgactttcc agatagaggg gggaaaggtg accgacgatt ccagagggat
      781 aatcgcacga gagaagccga tagactttga aagggtcaaa tcgtatctcg aaaggggcga
      841 aaaagccctg aaacggggtg cgtacgacga cgccgaagat tacgcgaaga gcgtgcttcg
      901 tatggacccg aacaacgccg ccgcgtaccg cctgataggc gaggccaaag acgcccgcaa
      961 cgatttcgcc ggagccgtgg agccgtacgc aaaagcagtg gaactggaac cgggagagcc
     1021 ttcgcactac ttcaagctgg ggcaggcgct ggcgaacatc cgcgccctcg acgaggcgaa
     1081 gaagatctat tcgatcatgc agcaggcttt ccctaacaac aagtggacga accagctcga
     1141 gatggcgata cgcttcatgt cgcgatagcg gagaggaaaa aagcgtcgag agagcccttg
     1201 cgccccaccg atccttggaa tgctctcgtt cgtcggtcga ttggaaagaa cgccgcgcgg
     1261 cgtcacgaac cgaagccgtc ggtatcggtt tattcgagaa agaagacgag ggggggattc
     1321 ttccctttct tgtcttcttt ctcgaaaacg cagactctcc ttgagcgcac cccttcgaga
     1381 ggccttccga acatcgcttg agtcgccttc cggaacaccg tgcgatcctc catgatgagc
     1441 tacaatagta cgaaatctct cccccgaaag gatgatcgcg cgtgcgaacg accgtcgcga
     1501 accggctctt gtactaccgg aacaccgtcg ccgacaccgg cagaatgaac cctgacatag
     1561 caacgcttcg aaaggagaac ctccttctgc tcctctcccg cgaggaggcg atgtcgggcg
     1621 ttccggagga cgccgaggcg gcgaaggctt ttcttgaacg ctccctgaag actccgaggg
     1681 agggcgcttc gggcggggac gacgacggaa gagtcgcagg cgttctctgc ccgtggatct
     1741 acaccccccg cgtcgtccac ggagccgctg cggagggcgc ccccttcgcc cttctctgcg
     1801 ttccggcaat tctctcctcc gaaggccgcc tccttccatc cggcgagcct ccctggattc
     1861 cctgaaccgt cctgcagccg ctgaacagcg aggggccgac cgtcggatcg gtcgaggact
     1921 ccgaccgttt cctcgcttcc ttggagagcg gttcggtcga gacatgggag gagcttgccg
     1981 cgacctgctc gcgctacata gccgccgtca ccgggagcga aacgcctccg gagatcgaag
     2041 ggtacctgat cgacgggcgc gcggccttct tcgccgcgga tacgatcaag gcgacgctcc
     2101 acttggccaa actctacgac gcccttctcg cggacaaggg ctccttccct ctgctcgagc
     2161 ggatgatcga gggggcgaac acctccgagc ccctcctctc gccagaggag gagatcgagg
     2221 tttcccgagg acacgtcggc tgcatgtccc cgaagtttcc tctctccccc tcgcagcgca
     2281 aggcgctcca tcacttcatg aggacgggga ggggagaggt gctcgcgctg aactgccatc
     2341 cgggaacagg caagaccacg atgctccaga gcgtcgtcgc gcagctttgg gtggacgccg
     2401 ccctgcgaaa gaccgaatgt cccctgatcg tcgcctcctc gacgaacaac caggcggtga
     2461 ccaacatcat cgacagcttc ggcaaggtcg caagggacga gaacgatcca ctctacgagc
     2521 gctggcttcc cggtataacc ggcttcggcc ttcagatggc gcgcgaggag agatggaagg
     2581 aggggttcca cgcctacgac aatccgttca gaaacggcaa gccggagacg agcggcctct
     2641 tcgagaagat agagcgtccc gagtggctcg aagaggccga agcgctcttt ctggagaagg
     2701 cgtcggagag gtacggcccc cttacgaacg tcgcctcggc gtcggaacgg ctgcacgcgg
     2761 agctttggga gtgcgcggag agcgtcggtt cgctcctcga cgccctcctt cgaacggcgc
     2821 cttcctcccc ggatgcgaag atcggcgagg agacgcgccg ccgcctcggg gaactcgaag
     2881 agagtctgga aagcgccgcg aaagaactcg agaacggaag acgtctcctc gcccgcgcag
     2941 aggagacccg tattctctgg gagaagcacc tcgcctccga gtcctttctc gtggcgctcc
     3001 tttcatttct gccacccgtg agaaaaagaa aagaggcggc ggaccgcgcc tttctgctcg
     3061 agcggagcat cgcctgcgaa tccgccgaca gagagaaaat ccgggaggcg cttgcccgcc
     3121 aaagagagga ggccgcgaag ggggcgtcgg aggcggaagc gagactgaac gagcgcacga
     3181 gggcgctctc gtcgctggag gagagctggt cgaaagctgc gtctctcgcc tcactcgccc
     3241 ggataaacct cgacgccgaa gacgtctcgc tgaacgaagc gaccgagcgt ctggacacat
     3301 ccgtccgggc gaaggccttc cgcgtagcga gccactactg ggaggccgag tacctgaggg
     3361 agcttcgagg caacatcgaa tcgaagtatc gggacagcgc cgcgcccgac aagagggcgc
     3421 gcatgtaccg ccgctttg
//
LOCUS       DAOSWW010000182         3423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19736_length_3423_cov_3.320368, whole genome shotgun sequence.
ACCESSION   DAOSWW010000182 DAOSWW010000000
VERSION     DAOSWW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19736_length_3423_cov_3.320368"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..788
                     /locus_tag="PK442_04900"
     CDS             258..788
                     /locus_tag="PK442_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO87018.1"
                     /translation="MKRRLGLLSDTHGHEDAWMKASLLWGNSLDAVLHAGDVLSSDVD
                     TGLAGMIRTAPFPVIISRGNCDYPEDEKLLHRPILSPYAFVWWNGKSILMAHGNAFEQ
                     FRSLALRSRVDLAVYGHTHVASVVREEGTLFVNPGSASLPRGRDPASAALIDEDGVVI
                     FTLDGAELHREQWIYS"
     gene            826..1350
                     /locus_tag="PK442_04905"
     CDS             826..1350
                     /locus_tag="PK442_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO87019.1"
                     /translation="MNPTLATKETQDLKAREVESRGTERITLVFALYEEDFGLDVTYV
                     REIVRVPPFITRVPNSPDYIRGVINLRGSIVPVFDMELKIGMMQKELTEEARIVVVSW
                     NEILFGIIVDSVREVCTIYDSQIESAAKLNTTMDKKYLLGVAKHEDGRLIVLLDLAAL
                     FDIDQILDEEAFNG"
     gene            1362..2234
                     /locus_tag="PK442_04910"
     CDS             1362..2234
                     /locus_tag="PK442_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO87020.1"
                     /translation="MIRIDLHLHSTFSDGSMTPEALVAHGRSKGVTVMSLTDHDTVDG
                     LPSFMAACARWNMAGIPGIELSADHARTVHILGYRLDLRRAGSASSASAQGNTPSMGM
                     KKMLEEVRRGRDERNAAMCENLRRMGLDITLDEVALEAGGDVVARPHMARVLVRKGYA
                     QDIVSAFARYLNTSSPAYVERYRLSPEDCIRMIADAGGLAVLAHPAQTTGDLSELREI
                     VRMLKDAGLWGIECVSSRASAEQIFRYLSLAAEFSLFPTAGNDFHGANRPSAMMGVAV
                     AEDFLPWARLGVSL"
     gene            2414..>3423
                     /gene="ltaE"
                     /locus_tag="PK442_04915"
     CDS             2414..>3423
                     /gene="ltaE"
                     /locus_tag="PK442_04915"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOO87021.1"
                     /translation="MFPVDLKSDTVTKPSEEMRKAMYAAEVGDDVYGEDPTVNRLEET
                     AAALTGMEAALFLPSGTMGNLVAVLTHCGRGDGAILGADCHILNYEGGGLAALGSVVP
                     LVADDSSGVISPEEVRTHCRPSNVHFAPARLLCLENTHNRRGGLALSPEAVRSSAQEA
                     HRLGLSVHIDGARIFNASTAWSCSVKKYAESVDSLQFCISKGLGAPVGSLLCGRRSFV
                     DAARHWRKRLGGGLRQSGILAAAGLYALEHNIERLREDHENAELLAHLLSEGTVVKVE
                     HNEKPTNMVYCAVPDGACADLHLRCADRGVLFNGVAENRFRLVTHLDVSREQVTRAAA
                     IIL"
BASE COUNT          684 a          928 c         1039 g          772 t
ORIGIN      
        1 gtgacagact ccaccagacg ggcggttacc agcccttcgg agtccagaag cctcctcacc
       61 tcttgaataa gcttgtgttc attacgttct tcagtcatgg gatacgctcc ttttgtattt
      121 tttcatactc tctccgtaag cgatgaaaaa gcccggagca tgctacaata aatgagtatc
      181 ggggcttttg ttcctctcca ttttagtaca ctttgccgta ttggggaaca tcccgttgtc
      241 aggaggggaa ccggataatg aaacgtcggc ttggtctgct gtcggacacg catgggcatg
      301 aagatgcctg gatgaaggca tccttgctgt ggggtaattc tcttgacgct gtcctgcatg
      361 caggcgatgt gctctcttcg gatgtcgata ccggattggc cgggatgatt cgcaccgcgc
      421 cgtttcctgt tataatttcg cggggaaact gcgattatcc cgaggatgaa aagttgttgc
      481 accggcccat tctttcgccg tacgcctttg tatggtggaa cgggaaaagt atccttatgg
      541 cgcatggaaa cgctttcgag cagttccgga gtcttgcgtt gcgaagcagg gtcgaccttg
      601 cggtgtacgg acatacgcac gttgcctccg tggtgcgcga ggaagggacc ctttttgtca
      661 accccggttc cgcgtctctt ccgaggggga gggatcccgc cagcgccgca ctcatcgacg
      721 aagacggcgt cgtcatcttc acgcttgacg gtgcggagct gcacagagaa cagtggatat
      781 actcatgata tattcgagag agattctacc gggaggggaa agaaaatgaa tccgacgctt
      841 gcgactaaag aaacacagga tttgaaagcc cgtgaggtgg aaagcagagg aacggagcgt
      901 atcacgcttg tgttcgctct gtatgaggaa gactttggtc ttgatgtgac gtacgtcagg
      961 gagatagtcc gggttccccc gtttatcacg agagttccca actctcccga ctatatccgg
     1021 ggcgttatca acttgcgggg atccatcgtt ccggtcttcg acatggagct gaagatcggc
     1081 atgatgcaga aggaactgac tgaggaggca aggatcgtcg tggtctcctg gaacgaaatc
     1141 ctcttcggca tcattgtgga ctccgtgcgg gaagtatgca ctatctacga cagtcagatc
     1201 gagtccgccg ccaagctcaa cactaccatg gacaagaagt atctcctcgg tgtggcgaag
     1261 catgaagacg ggcgtctgat cgtgctgctc gatcttgctg ccctgttcga catcgaccag
     1321 attctcgacg aagaagcatt caacgggtga gtcggggccg tatgatccgt atcgacctgc
     1381 atctccacag caccttctcg gacggctcga tgacgccgga agccctcgtg gcccacggca
     1441 gaagtaaagg cgtgaccgtc atgagtctga ccgatcacga tacggtcgac gggctccctt
     1501 cgttcatggc ggcgtgcgcg cgatggaaca tggccggtat tccgggaata gaactctcgg
     1561 ccgatcatgc gcgtaccgtc cacatcctgg ggtatcgatt ggatcttcgc cgcgcaggga
     1621 gcgcatcttc cgcttccgcg caggggaata cgccttccat gggcatgaaa aaaatgcttg
     1681 aggaagtgcg tcggggaagg gacgagcgga atgccgcgat gtgcgagaat ctgcggagga
     1741 tggggctcga cattactctg gatgaggttg ccctggaggc gggcggcgat gtcgtcgcgc
     1801 gcccccacat ggcaagagtg cttgtgcgaa aaggatacgc tcaggacatt gtttctgcct
     1861 tcgcacgtta cctgaacaca tcctctccgg cgtatgtgga acgctatcgt ctttcgccgg
     1921 aggactgcat ccggatgatc gccgatgcgg gagggctcgc cgttctcgcc caccccgcgc
     1981 agactaccgg cgatctttcg gaactccggg agatcgtccg gatgctcaag gacgccggat
     2041 tgtggggaat tgaatgcgtc tcctcgcggg cttccgcgga gcagatcttc cgctaccttt
     2101 cacttgcagc ggaattttct ctcttcccga cggcgggcaa cgatttccac ggcgccaacc
     2161 gtccgtccgc catgatgggc gtggcggttg cggaggattt cctcccttgg gcgaggctgg
     2221 gcgtttctct gtaacgtcct gcttgcaggg gcttcgatga agctgcgggc gaatttcggc
     2281 gggaactacc ctccggaatt cgctcttttg atggtattat atcagccgga gcttttgtat
     2341 aatgttgatt tgagaatgta attgccacac ggcatattcg tgaacgctct ggtttaaagg
     2401 aggaattgga cacatgtttc cagttgatct taaaagcgat actgtaacga agccttcgga
     2461 ggagatgcgg aaggctatgt atgctgcaga agtcggcgat gacgtctatg gcgaagatcc
     2521 caccgtcaat cgcctggagg agacggccgc cgcgctcacc ggcatggagg ccgccctgtt
     2581 tctgccctca ggaaccatgg ggaacctggt ggcggtgctc acgcactgcg gcagagggga
     2641 cggcgcgatt ctcggcgcgg attgccacat actgaattat gaaggcggcg gtctggccgc
     2701 tctcggctcc gtcgttccgc tggttgccga cgacagctcg ggggtgatct ctccggagga
     2761 agtgcgcaca cactgtcgtc cgtcgaatgt tcacttcgcc cctgccaggc tgctctgcct
     2821 tgagaacacg cataaccgca gaggaggact ggcgctctct cccgaggccg ttcggtcttc
     2881 ggcgcaggaa gcccatcgtc tcggtctctc cgtgcatatc gacggagcga ggatcttcaa
     2941 cgcatccacg gcatggagct gttctgtgaa gaagtacgcg gaaagcgtgg attcgcttca
     3001 gttctgcatc tcaaaggggc ttggcgcacc ggtcggaagc cttctgtgcg ggaggcgcag
     3061 ctttgtcgac gccgcccgcc attggaggaa acgtcttggc ggcggactgc ggcagtccgg
     3121 cattcttgct gccgccggtc tgtacgcgct ggagcataat atcgaacgtc tccgggagga
     3181 tcacgagaat gcggaactgc tcgcgcatct tctctccgag ggaaccgtcg tcaaggtcga
     3241 gcacaatgag aaaccgacga acatggtgta ctgcgccgtt cccgatggcg cgtgcgccga
     3301 tctgcatctc cgctgcgccg acagaggcgt cctgttcaac ggcgttgcgg agaaccgttt
     3361 ccgccttgtg acgcatctcg acgtgtcgag agaacaggta acccgggccg cggcgatcat
     3421 cct
//
LOCUS       DAOSWW010000183         3414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_19826_length_3414_cov_3.529622, whole genome shotgun sequence.
ACCESSION   DAOSWW010000183 DAOSWW010000000
VERSION     DAOSWW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19826_length_3414_cov_3.529622"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PK442_04920"
     CDS             <1..409
                     /locus_tag="PK442_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO87022.1"
                     /translation="VEALASRPMGELSQGQRQRVLIARALASEPKVLLLDEPLASIDP
                     ETRDMLLTELAALRARMTVIMVSHDISAIPGRATAVACVNRNVFYHDSGEILPGMFEK
                     VWGTCPVELVAHGIPHRVLAVHTHDHSEDCAHD"
     gene            402..1202
                     /locus_tag="PK442_04925"
     CDS             402..1202
                     /locus_tag="PK442_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOO87023.1"
                     /translation="MIELLQFEFMRNALWAALLCSGLCGILGTVVVVKRYVILAGGVA
                     HAAYGGVGIALYLGISPQLGAVGFSLALSLLMAHVMLKREARADSIIGVLWAAGMAAG
                     VIFADLTPGYGADLMSYLFGSILTVAKADLFMMGGLLAVTLVLGFVWRRQIAAFSFDE
                     DFARTRGVPVTLLHFGAVVLLSMAVVLIIRIVGLILVIALLTIPPSVAEKFSRSLWSM
                     MGISCVLSALFSVAGLALAVQFNLTAGAVIIAVASAVYLVSLLLPGRR"
     gene            complement(1271..1672)
                     /locus_tag="PK442_04930"
     CDS             complement(1271..1672)
                     /locus_tag="PK442_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOO87024.1"
                     /translation="MPEICSQITFFYYKNFERARDFYERIMGFELADDQGTCRIYRVQ
                     GTSFLGIVDEKHGHCSAPRSESNVLVTLATNDVKGWWEHLRKEGVKITSELLWKPEIS
                     IEAFFFEDPEGYALEVQSFLKPELRKIFGQE"
     gene            complement(1711..2724)
                     /locus_tag="PK442_04935"
     CDS             complement(1711..2724)
                     /locus_tag="PK442_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87025.1"
                     /translation="MRTDTNAHTPPAATLLSPEAFLYSASIPGFFSALPLDERSSLSV
                     ATGRSALAKALDTASQKALTGRAWLPSLCCASLAPAFLQRGFSISFYSTAPFIPPRLE
                     RGDVFLYIHFCGFPNRIAERELGTLPEERRPIVVEDCVHALFTRGTGRFGDFVLYSFR
                     KFLPVPDGGLLLSREPLDDTLSPPLERFISLKTAGLLSESRRLLEEGEEALDADDEAR
                     EPSVMGALLLDWLPWTHIPEARRRNYAFLAERLRLPPLDDDTVPLGAPLRISAGKAEL
                     ERRLRASGFEPPLSWDAPRDAPSDEADRLVVLPCDERMEERELARLVRICKTFSAERL
                     AAR"
     gene            complement(2738..>3414)
                     /locus_tag="PK442_04940"
     CDS             complement(2738..>3414)
                     /locus_tag="PK442_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOO87026.1"
                     /translation="VLDAPREVALLLPNFTGWNGLPIRKMLSSKIGLPVELENDANCY
                     ALGEGWGGAARGMTDYILLTLGTGIGGGIVIGGKILKGFHGMAGEPGHLVVGSGEPCG
                     CSSHGHLEGISGADALERTAKSMGLQPDLKYLWTRRTDPAVAPLWDRGLDHLAKGIAS
                     AIHLFDPQAVILGGGMSRGEGFIDVLKPRVVDYLALPFRKTLDLRPSLLGNDAPTIGA
                     AALTRK"
BASE COUNT          553 a         1071 c         1151 g          639 t
ORIGIN      
        1 ggtggaggct ctggcctccc ggccgatggg ggaactctcc caggggcagc ggcagcgtgt
       61 gctcatcgcg cgggcgctgg cttcggagcc gaaggtgctg ctgctcgacg aaccgttggc
      121 gagcatcgac ccggagacgc gcgacatgct gctgacggag ctggccgcgc tccgcgcacg
      181 gatgacggtg atcatggtga gccacgacat ctccgccatt ccggggcgcg ccacagccgt
      241 ggcgtgcgtc aaccggaacg tcttctacca cgactcgggg gagattctcc cgggcatgtt
      301 cgagaaggtc tgggggacgt gtcccgtgga gctggtggcg cacggcatcc cgcacagggt
      361 gctcgccgtt cacacgcacg accattccga ggactgcgct catgattgag ctgctccagt
      421 tcgagttcat gcggaacgcc ctctgggcgg ccctgctgtg cagcgggctc tgcggcatcc
      481 tcgggaccgt ggtcgtggtg aagcgctacg tcatcctcgc ggggggcgtc gcgcatgcgg
      541 cgtacggcgg cgtgggcatc gcgctctacc tcgggatttc accgcagctc ggggccgtgg
      601 gcttctcgct ggctctttcg ctgctgatgg cgcacgtgat gctgaagcgg gaggcgcggg
      661 cggactccat catcggtgtg ctctgggccg caggaatggc ggcgggcgtc atcttcgccg
      721 accttacgcc ggggtacgga gccgatctga tgagctatct cttcggcagc atcctcaccg
      781 tcgccaaggc cgacctcttc atgatgggcg gactgctcgc ggtgacgctc gttctcggat
      841 tcgtctggcg caggcagatc gccgccttct cgttcgacga ggacttcgcc cgcacgcggg
      901 gcgttcccgt cacgctcctc cacttcggtg cggtggtgct gctctcgatg gccgtggtgc
      961 tgatcatccg catcgtcggg ctgattctgg tcatcgcgct gctcaccatc cccccatccg
     1021 tggcggagaa gttctcccgc tcgctttggt cgatgatggg catttcgtgc gttttgagcg
     1081 cgctcttctc cgtcgccggg ctcgccctcg cggtacaatt caacctcacc gcgggcgcgg
     1141 tgatcatcgc ggtggcctcg gcggtctatc tggtgtcgct gctactgccg gggagacggt
     1201 aactcccctc ctccgctctt tgtacttcgg aggggagcta ccgcttcacg tttcgccgcg
     1261 tagcgccgta tcactcctgc ccgaaaatct tccgcagctc cggcttcagg aaagactgga
     1321 cttcgagggc gtacccctcc ggatcctcga agaagaacgc ctcgatagaa atctccggtt
     1381 tccaaagcag ctcggaggtt atcttgacgc cttccttccg caagtgctcc caccacccct
     1441 tgacgtcgtt cgtcgccagc gtgacgagga cgttgctctc cgagcggggc gcggagcagt
     1501 gcccgtgctt ctcgtcgacg attccgagga agctcgtccc ctgtacccgg tagatacggc
     1561 acgtcccctg gtcgtccgcg agttcgaacc ccatgatccg ctcgtaaaag tcgcgcgccc
     1621 tctcgaagtt cttgtagtaa aaaaaagtga tctgtgaaca gatttcaggc atgaagcatt
     1681 cctcccattg atttctgcct tgctgcgccg ttatcgtgca gcaaggcgct ccgctgagaa
     1741 cgttttacag atgcgcacga gccgcgcaag ctcccgctcc tccatccgtt cgtcgcacgg
     1801 caaaacgacg agcctgtcgg cctcgtccga gggagcgtcg cggggagcgt cccatgagag
     1861 aggcggctcg aagcccgacg cccgaaggcg gcgttcgagt tcggcttttc ccgcggatat
     1921 gcggagcgga gccccgagcg gaaccgtgtc gtcgtcgagc ggcggcaggc gcaggcgttc
     1981 cgcgaggaag gcgtagttcc gtctgcgagc ttcgggaatg tgcgtccacg ggagccagtc
     2041 gaggaggagc gctcccataa cagaaggctc tctggcctcg tcgtccgcgt cgagcgcctc
     2101 ctctccctct tcgagaaggc ggcgcgattc cgacagaagc cccgccgtct tgagggagat
     2161 gaagcgttcg agcggcggag agagcgtgtc gtcgagcggc tcgcgggaga ggagcagccc
     2221 accgtccggg acggggagaa acttgcggaa gctgtagagc acgaagtcgc cgaagcgtcc
     2281 tgttcctcgt gtgaagaggg cgtggacgca gtcctcgacg acgatcgggc ggcgctcctc
     2341 cggcagcgtg ccgagctccc gctcggcaat cctgttcggg aagccgcaga agtggatgta
     2401 caggaagacg tcccctcgtt caagcctggg ggggataaac ggcgccgtac tgtagaagga
     2461 gatggagaac cctctctgca aaaaagcggg ggcgagcgac gcgcagcaga gcgaagggag
     2521 ccacgccctc cccgtaagcg ctttttggga agcggtgtcg agcgcctttg caagcgctga
     2581 tcttcctgtg gcgacggaga gcgaggagcg ttcgtcgagc gggagggcgg agaaaaaacc
     2641 ggggatggag gcggagtaca aaaaagcctc cggagacagc aacgtggccg ccggaggcgt
     2701 atgagcgttt gtatcggtgc gcatgtctgc gaggcgctta cttccgagtg agggccgcgg
     2761 cgccgatggt gggcgcgtcg ttgccgagga ggctcgggcg cagatcaagc gtcttgcgga
     2821 aagggagcgc aaggtagtcc actactcttg gcttcaggac gtcgataaac ccctcgccgc
     2881 ggctcatgcc gccgccgagg atgaccgcct gcgggtcgaa gaggtgaatc gccgaggcga
     2941 tccccttcgc caagtggtcc agccctcggt cccacagagg cgctacagcc gggtcggtcc
     3001 tgcgggtcca gaggtacttc aggtcgggct gcaaacccat cgacttcgct gtgcgctcca
     3061 gggcgtccgc gccgctgatg ccttcgaggt gcccgtggga ggagcagccg cacggctctc
     3121 cggagccgac gacgagatgt cccggttcgc ccgccatgcc gtggaagcct ttgagtattt
     3181 tgccgccgat aacaatgccg ccgccgatgc cggtgccgag cgtgaggagg atgtagtcgg
     3241 tcatcccccg ggctgcgccg ccccagcctt cgccgagcgc gtagcagttg gcgtcgtttt
     3301 cgagttccac cggcagccct atcttcgatg agagcatctt cctgatcggc aggccgttcc
     3361 agccggtgaa gttcgggagg agcagcgcaa cctcgcgcgg ggcgtcgaga acgc
//
LOCUS       DAOSWW010000184         3395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20010_length_3395_cov_3.381138, whole genome shotgun sequence.
ACCESSION   DAOSWW010000184 DAOSWW010000000
VERSION     DAOSWW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20010_length_3395_cov_3.381138"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1790
                     /gene="rfaE2"
                     /locus_tag="PK442_04945"
     CDS             360..1790
                     /gene="rfaE2"
                     /locus_tag="PK442_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOO87027.1"
                     /translation="MILHSEKFKDIRIAVVGDVVLDKYVSGIARRISSEAAIPVLLAR
                     RIGHVAGGAANVAANLSGIGCSVSVYGAVGDDGNSVLLSELLDEKKIEHKLQQINGRP
                     TTIKTRIVAGNQQIARYDIEESSALSKHDEDLLFSSLLEEMDGLDGIILSDYGNGVLK
                     GSLCSRIIEAAATKGTPVFVDPRGNNWNRYRGAACATPNEAELALVSEGAEIDEQTLS
                     LHAHSARKRYGLRNLLVTRGAKGMALFLEDGEEFFLPAFCVREVFDVSGAGDTVIAVL
                     AASLIAGATWKQAADWANRAAGVVVTRAGTSPVSLEDLLALNQVERSGKVVSFEEATA
                     RVEDWKREGKTVVFTNGCFDLLHSGHVHLLRHAAREGDRLVVGVNSDASVQRLKGPSR
                     PVASQEDRLSVLSALECVDLVVLFEEDTPLRLIQALRPSVLVKGADYTLPQVVGRDVV
                     EEAGGRVVLAPLLEGRSTTNIIKSCR"
     gene            complement(1774..2748)
                     /locus_tag="PK442_04950"
     CDS             complement(1774..2748)
                     /locus_tag="PK442_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOO87028.1"
                     /translation="MSTLHHEDMIFVAGHRGLVGSAIVRKLREKGYDNLLLKTRSELD
                     LTDQAAVRAFFKSERPDHVVLAAAKVGGILANSTYPADFIYQNLAIEVNIIHEAWLAG
                     VKKLLFLGSSCIYPKLAPQPLKEEYLLTSSLEPTNEAYAIAKIAGLKMCEFYNRQYGA
                     RFISVMPTNLYGPGDNFDLNTSHVLPAMIRKFHEAKTRGDGSVTLWGTGTPRREFLHV
                     DDLADACVFLLERYEDPAFLNIGVGEDVTIRELAELVRRIVGFGGDILWDSAKPDGTP
                     RKLLDVSKLKALGWKPAISLEEGIEDTRRWYLRNEAEPALRPGVIGTT"
     gene            complement(2764..>3395)
                     /locus_tag="PK442_04955"
     CDS             complement(2764..>3395)
                     /locus_tag="PK442_04955"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOO87029.1"
                     /translation="YAAAKLYGYWITVNYREAYGMFACNGILFNHESPLRGETFVTRK
                     ITRAAARIALGLQEKLHLGNLDAKRDWGHARDYVEAMWLMLQQDEPDDYVVATGETHT
                     VREFAERAFAEAGIPLAWKGTGVDEQGVHAESGAVLVEVDPRYFRPTEVELLLGDPSK
                     ARERLGWKPRVAFRELVAEMVRADLDLFRRDLICRDAGYSVCAAAEDRC"
BASE COUNT          684 a         1034 c         1013 g          664 t
ORIGIN      
        1 cgtacgccaa ccatgtcaca tgacttcggg ggtacctctc cttgagcgcc ctgcaataag
       61 gcgtaactat cagaatatcg ccgagcgcgc tgtaacgcag aaagagaatg cgttcccctt
      121 cgccgtcttt cgaagggggg gtataggtcc tgcaagaacg gatcatgcac ggaaccagga
      181 acaaaaaaac aaatgaagaa ccgccacgga acagtcactc cttcgataac gccacgtaga
      241 aagaaccggc ggggaaaatg cgctccgctt cgtattctat caaagatatt gtaaaatgga
      301 gcttgaaaaa accgaacgtg aatttgccca tctttcgata cttcaaggag cgagtcgcaa
      361 tgatactcca tagtgaaaag ttcaaggaca ttcgtatcgc cgtcgtggga gacgtcgttc
      421 tcgataagta cgtcagcgga atcgcgcggc gtatatcctc cgaggccgcc attccggtgc
      481 ttctcgctcg aagaataggt catgtcgcgg gcggagcggc gaacgtcgcg gcgaacctgt
      541 cggggatcgg gtgttcggtc tccgtgtacg gcgctgtcgg cgacgacggg aacagcgtcc
      601 tgctgagcga gctcctcgat gaaaaaaaga tagagcacaa gctgcagcag atcaacggcc
      661 gccccacgac gattaaaacc cgcatcgttg ccgggaatca gcagatcgcg cgctatgata
      721 tcgaagagtc ctcggcgctc tcgaaacacg acgaagacct gctcttctca tccctgctgg
      781 aagaaatgga cggtctcgac ggcattatcc tctccgatta cgggaacggc gttttgaaag
      841 gttctctctg ctcaagaatc atcgaagcgg ccgcaacgaa gggaaccccc gtcttcgtcg
      901 accccagggg gaacaactgg aaccgctatc ggggcgccgc ctgcgccacg ccgaacgagg
      961 cggagctggc cttggtctcc gagggcgcgg agatcgacga acagacgctc tcgcttcatg
     1021 cgcattcggc acggaaacgc tacgggctcc gaaatctgct ggtgacgcga ggggccaaag
     1081 gaatggcact gttcctcgag gacggggaag agttcttcct tcccgcgttt tgcgtgcgcg
     1141 aggtgttcga cgtttccgga gcgggcgata cggtcatcgc cgttcttgcg gcatcgctga
     1201 tcgcgggggc gacctggaag caggccgccg actgggcgaa ccgggccgcc ggcgtcgtgg
     1261 tcacgcgcgc cgggacctcc cccgtctccc tggaggattt gcttgcgctc aaccaagtgg
     1321 aaagaagcgg caaggtcgtc tccttcgaag aagcgaccgc cagagtcgag gattggaagc
     1381 gagaggggaa aaccgtggtg tttacgaacg gctgcttcga cctgttgcac tccgggcatg
     1441 ttcatctgct ccggcatgcc gcgcgcgaag gcgatcgcct tgtcgtcggc gtcaattcgg
     1501 acgcgtccgt gcaacggctc aaagggcctt cccgccccgt ggcttcccag gaagatcgcc
     1561 tttccgttct ttccgctctg gagtgcgtcg atcttgtcgt cctcttcgag gaggacacgc
     1621 cccttcgtct tatccaggcg ctccggccgt ccgtcctggt caaaggtgcg gactatacac
     1681 tgccgcaggt cgtgggaaga gatgtcgtgg aagaagcggg aggccgcgtg gtgctggccc
     1741 cgcttcttga agggcgaagc acgacgaata tcatcaagtc gtgccgatga cgccgggacg
     1801 aagagcgggt tcggcttcgt tccggaggta ccaccgccgc gtatcttcaa tgccttcttc
     1861 gagagaaatg gccggtttcc agccgagcgc tttcaatttc gacacgtcga gcagcttccg
     1921 cggcgtcccg tccggcttcg cggagtccca gaggatatct ccgccgaaac cgacgatccg
     1981 ccgaaccagc tccgcaagtt cgcgtatcgt cacgtcctcg ccgacgccga tattgagaaa
     2041 agcggggtct tcgtagcgct ccagcagaaa gacgcaggcg tcggccaggt cgtccacatg
     2101 caaaaactcc cttcggggag ttcccgtccc ccaaagagtc accgagccgt ctcccctcgt
     2161 cttcgcctcg tgaaatttcc tgatcatcgc gggcagcacg tgcgacgtat tcaaatcgaa
     2221 gttatccccc gggccgtaga ggttggtggg cataacggag ataaaacggg cgccgtactg
     2281 ccggttataa aactcgcaca tcttgagtcc ggcgatcttc gcgatggcgt acgcttcgtt
     2341 cgtcggctca agcgacgagg tcagaaggta ttcctctttt aaaggctgcg gcgccagctt
     2401 cggatagatg cacgagctgc cgaggaagag aagcttcttc acgcccgcga gccacgcctc
     2461 gtggatgata ttgacctcga tcgcgaggtt ctggtagatg aaatccgcag gatatgtcga
     2521 attggccagt attcccccga ccttcgccgc cgccaggacc acgtgatcgg ggcgttccga
     2581 cttgaagaag gcgcgcaccg cagcctggtc ggtcaggtcg agctcgctgc gggttttgag
     2641 caggagattg tcatacccct tttcacgcag cttcctgacg atcgccgagc cgacgaggcc
     2701 cctgtgcccc gcgacaaaga tcatatcttc atggtgaaga gtcgacatcg tgttcacctc
     2761 cgttcaacag cggtcttccg ccgccgcgca gacgctgtac cccgcgtcgc ggcaaataag
     2821 gtctctccgg aagagatcga gatcggcgcg caccatctcg gcgacaagct cccggaaggc
     2881 gacgcggggc ttccacccca gtcgttcccg tgccttcgac ggatcgccga gcagcagttc
     2941 gacctcggtc gggcggaagt acctcggatc cacctcgacg agcacggctc cgctttctgc
     3001 gtgcacccct tgttcgtcga cgccggttcc tttccatgcg agagggatgc ccgcctcggc
     3061 gaaggccctt tcggcgaatt cgcggaccgt gtgcgtctcg ccggtcgcca cgacgtagtc
     3121 gtccggctca tcctgctgaa gcatcagcca catcgcctcg acataatcgc gcgcgtggcc
     3181 ccagtcgcgc ttcgcgtcaa ggttgcccag gtgcagcttc tcctgcaagc cgagggcaat
     3241 gcgcgccgct gcccgtgtga tcttgcgggt gacgaacgtc tccccgcgca ggggcgattc
     3301 gtggttaaac aaaataccgt tacaggcgaa catcccgtat gcctcgcggt agttcacggt
     3361 gatccagtac ccgtacagct tcgccgcggc gtagg
//
LOCUS       DAOSWW010000185         3389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20070_length_3389_cov_3.576185, whole genome shotgun sequence.
ACCESSION   DAOSWW010000185 DAOSWW010000000
VERSION     DAOSWW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20070_length_3389_cov_3.576185"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PK442_04960"
     CDS             complement(<1..1394)
                     /locus_tag="PK442_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOO87030.1"
                     /translation="MEKWNEELLKSIESIASEAGVRPDGKDSGTMEGLKPEELHSNDI
                     VFRSTSRAVVVCRMLFNARGIPVDFLLESVNPAFCRIFGIAPESAAGRSGKEFFMPDG
                     KEIPALDLFDNALKEQQAFSFMFSSEKLSKVFDVLAFSLENGTFCMAITDATSQVAAE
                     RERRENERFTEKVFGSVDVWIVLLDERGRVVFWNAAAERLSGISADEALSGSFDFRSV
                     FCPGEEDREDAIGKMDACRRDGATEVRLETCVCAAEGRSLRVEWTVRPWHFEDVSSDG
                     LLLLGRDVTQDRHTEEQLLESERRYAAAFKETMAPMLLVDPETGAVCDCNAAALALYG
                     YSREEMLALTNRDFNLLPAEDLRKNMEEATARLRNRFVFQHRLKNGDIRTVEVFASPT
                     PFQGKTFLFSILHDITEKQRLEEQLHLVTFDCALQAKILGGILESSPDYLYVFDRMRN
                     FRFSGKKGAALFERT"
     gene            complement(1426..2946)
                     /locus_tag="PK442_04965"
     CDS             complement(1426..2946)
                     /locus_tag="PK442_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOO87031.1"
                     /translation="MSDMRMANIDKLTLEIEKLLADAEVTGALPQVEDRDASWEVRIS
                     DDGLTAYLDMYPAVGNGVSPDPLGICTELEEMGIRRVDRDRVMALVTLCDSGYPASGE
                     DAVVARGVPPESPSPGRIEFLVLMERAKRSEDDEASVDWKNLWLTPSVHEGDVIAKIY
                     PPKEGTDGVDVYGNPLPAASHTFFRIRYGDGVNVSEGEDGTVEIASARTVGQPVFRDN
                     VLDVAPLLVINGDVNISTGSIDFTGSVLVSGSVLEGFSVKAERDVNVQGWVYNARVQA
                     GGSCSVKGGVTGERSLLLAGEDVRVGVVEYAEISASRKVEVFGYALFAMLEAGESIYV
                     QGRNRRGVVGGTCIAGACIEALSAGSPMESSTQLEAGRDPFQARILQELDRKKEALLA
                     MQKKIEQAILSIKGTGPNFDIDTLTEEEKGKLFLLVQYHGKMKSSVAELAARIDDEKR
                     ASIAEKRIMPRIRIRDRVYPNVTLKIWGQTLVVKQQEFHVSFFMDKDRGVIARGGF"
     gene            complement(3073..>3389)
                     /locus_tag="PK442_04970"
     CDS             complement(3073..>3389)
                     /locus_tag="PK442_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOO87032.1"
                     /translation="LHHRRQYPEDAPGGVTVEAWVYILRCADGTLYTGWTFDTEARLC
                     VHNSGKGARYTASRLPVEMVYRERVASKSEALRRERRIKRMRRSAKERLIAGAFSVSL
                     PE"
BASE COUNT          713 a         1071 c          885 g          720 t
ORIGIN      
        1 gttctttcaa agagcgccgc ccctttcttt ccggaaaaac ggaaatttct catgcgatca
       61 aaaacgtaga gatagtccgg cgaactctcc agaataccgc cgagaatctt cgcctgcagg
      121 gcgcagtcga aggtgaccag atggagctgt tcctcgagac gctgtttctc ggtgatatcg
      181 tgaagaatag aaaagagaaa ggtcttcccc tgaaacggag tcggcgaagc aaagacctcc
      241 actgtgcgga tgtcgccgtt cttgagacga tgctggaaaa cgaaccggtt cctgagccgc
      301 gccgtcgcct cttccatgtt cttgcgcaga tcttccgccg gaagcagatt aaaatctcgg
      361 ttcgtcaagg cgagcatctc ttctctggaa tagccgtaga gggcaagggc ggcggcgtta
      421 cagtcgcata cggcgcccgt ttccgggtcg acgagaagca taggggccat ggtctccttg
      481 aaggcggcgg cataccggcg ctcgctttcc aaaagctgct cctcggtgtg gcgatcctgc
      541 gtcacatcgc gcccaaggag cagaagaccg tcggaggaga catcctcgaa atgccacggc
      601 cgaaccgtcc actccacacg aagcgatctt ccctcagccg cgcacacgca ggtttccagc
      661 cggacctccg tcgcaccgtc ccttcggcag gcatccatct tcccgatggc atcttcacga
      721 tcctcttctc cgggacaaaa aacgctccgg aaatcaaagc tccccgagag cgcttcgtcg
      781 gccgagatgc cggacaagcg ctccgcggcg gcgttccaga agacgactct tccccgttcg
      841 tcgagcagca cgatccagac atccacgctt ccgaagactt tttccgtaaa ccgttcgttc
      901 tcccgccgct ccctttccgc ggcaacctga ctggtcgcat ccgttatggc catacaaaac
      961 gtgccgtttt cgagagagaa cgcaagaacg tcaaaaacct tgctcaattt ttctgaggaa
     1021 aacatgaaag aaaaggcctg ttgttctttg agagcattgt caaaaaggtc cagggcggga
     1081 atctccttgc catcgggcat gaagaactcc tttccgctcc tgcccgccgc gctctccggg
     1141 gctatgccga aaatcctgca aaaagcgggg ttgacgctct ccagcagaaa atctaccggt
     1201 atcccccgcg cattgaagag catacggcag acaaccactg cgcgtgaggt agaacggaaa
     1261 acgatatcgt ttgaatgcaa ctcttcaggc ttcaaaccct ccatagttcc tgagtctttc
     1321 ccgtcgggac gcactccggc ttcggaggct atcgactcga tgctcttcag cagctcctcg
     1381 ttccacttct ccatcacgca ctcctccttc cggctgttcc cgctctcaga aaccgcctct
     1441 tgcgatcacg ccgcggtctt tgtccataaa gaaagacaca tggaattcct gctgcttgac
     1501 caccagcgtt tgtccccaaa ttttcagggt gacgttgggg tagactctgt cgcggatgcg
     1561 gatgcggggc atgatgcgct tttccgctat ggacgcgcgt ttttcatcgt cgatccttgc
     1621 ggccagctcc gctacggaac tcttcatctt gccgtggtat tgaacgagga gaaaaagctt
     1681 gcccttttcc tcctcggtaa gagtgtcaat atcaaaattc gggcctgttc ctttaattga
     1741 aagaatcgct tgctctatct tcttctgcat cgcaaggagc gcctctttct tccggtcaag
     1801 ctcctggagt attctggcct ggaacgggtc tcgtcccgcc tcaagctgcg tggacgactc
     1861 catcggagag cctgcggaga gcgcctcgat gcaggcgccg gcgatacagg ttccgcccac
     1921 gacgcccctg cggttcctcc cctggacgta gatcgattcg cccgcctcaa gcatcgcaaa
     1981 gagcgcgtag ccgaagactt cgaccttccg tgaggcgctt atttccgcgt attcaacaac
     2041 gccgacgcgc acgtcttcgc ctgcgagaag caacgagcgt tctcccgtca cgcccccttt
     2101 tacactgcac gagccccccg cctgcactcg cgcgttatac acccacccct gcacattgac
     2161 gtctctctcc gccttgacgg aaaaaccttc gagcaccgaa ccggagacaa ggacggaacc
     2221 ggtaaaatca atgctgccgg tggagatgtt tacgtcgccg ttgatgacca gcagaggcgc
     2281 cacatccagc acattatccc ggaagacggg ttggccgacc gtccgtgccg atgcgatttc
     2341 aaccgtgccg tcttcaccct ccgagacgtt cacaccgtcg ccgtaacgaa tgcggaaaaa
     2401 cgtgtgtgaa gcagcaggaa gaggattccc gtagacatcc accccgtcgg ttccctcttt
     2461 cggaggataa atcttcgcga tgacgtctcc ttcgtgcacg gagggggtga gccagagatt
     2521 tttccagtcg acggacgcct cgtcgtcctc ggagcgcttc gccctttcca taagaaccag
     2581 aaactcgatc cggcccgggc tcggcgactc cggcggaaca cctctcgcga cgacagcgtc
     2641 ctcccctgag gccgggtatc cggaatcgca cagagtaacc agcgccatca ctctgtcgcg
     2701 gtcgactcgg cgtattccca tctcttcaag ctccgtgcag attccgagcg gatcggggga
     2761 aacgccgttt cccactgcgg ggtacatgtc gagatacgct gtcaagccat cgtcagaaat
     2821 acgcacctcc cacgacgcat ccctgtcctc cacctgaggg agagctcctg ttacctccgc
     2881 gtcggcgaga agcttctcga tttccaaggt cagtttgtcg atgttggcca ttcgcatatc
     2941 gctcatacgg aagaccccct ctcattttcc acaacacaag agcataccca gcttcagaat
     3001 actctttttt ctccggaagg gcaagaagag ggttcgaacc gctgggtatt ttgcgaagaa
     3061 gaataacaag ggctattccg ggagactcac cgagaaggcg ccggcaataa gccgctcttt
     3121 cgcgctgcgg cgcatcctct tgatccgccg ttcccggcgc agcgcctcgc tctttgaggc
     3181 cacgcgttcc ctgtacacca tttcgaccgg aaggcgcgat gcggtgtacc gcgcgccctt
     3241 gccgctgtta tggacgcaga gccgagcttc cgtatcgaag gtccagccgg tgtagagcgt
     3301 cccgtcggcg cagcggagga tgtagaccca cgcctccacg gttacgccgc caggtgcatc
     3361 ctcaggatat tggcgccgat gatgcagag
//
LOCUS       DAOSWW010000186         3386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20108_length_3386_cov_4.709697, whole genome shotgun sequence.
ACCESSION   DAOSWW010000186 DAOSWW010000000
VERSION     DAOSWW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20108_length_3386_cov_4.709697"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PK442_04975"
     CDS             complement(<1..756)
                     /locus_tag="PK442_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOO87033.1"
                     /translation="MPCSICLHGHFYQPPRENPWLEEVEIQESAAPWHDWNERISEEC
                     YGPNSAARIFDDAGWIRRIVNNYSRISFNFGPTLLSWMERKRPQYYQAVLEADRLGSA
                     RFSGHGPAIAQAYNHMILPLANRRDKITQIVWGIEDFQKRFQRAPEGMWLPECAVDSE
                     TLDILAEHGIRFVILAPRQAQAVREKGASSWTDVTGERVDTHRAYICPLRSGRALSLF
                     FYQGELAQKVAFGDALRNGGDFAKMLIGALPESA"
     gene            817..2337
                     /gene="malQ"
                     /locus_tag="PK442_04980"
     CDS             817..2337
                     /gene="malQ"
                     /locus_tag="PK442_04980"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOO87034.1"
                     /translation="MYNKEQKTEVFFLTRSCGILLPVSSLPGRWGIGDLGPGARSFVD
                     FLSRSGQKTWQILPLSETDGALGNSPYSSPSAFAGNHLFISPDDLFKCGLLTAADTAE
                     LPPFPGEKVDYPTVRAVKEKLLARAFERFAPSEEYETFLQQNEHWVFDYALFSALKQR
                     FGGLSWNQWPDELKLRDGNALRTAATELRESVDFLLFKQYLFFSSMERLRRESDEKGV
                     ELLGDLPIYVNFDSADVWAHPNLFHLDENLLPVEVAGVPPDYFSETGQLWGNPLYNWE
                     ALREDDFSWWIKRLQHSLSMFHLVRIDHFRGLLAYWAVPFGDRTAEKGVWRDVPSEAF
                     FASLRKNIPSASFLAENLGVITPDVTDAMERMSFPGMAVVLFAFGGGMKDNPHIPHNY
                     SATLAAYSGTHDNNTALGWYRDDASEEERNTLGRYLGRMPDEKEVPDIIVRMVLSSVA
                     QRAIIPLQDYLGLGSEARMNTPATPSGNWEWRADELCFTEELSIRIKQLSEIYGRN"
     gene            2524..>3386
                     /gene="glgP"
                     /locus_tag="PK442_04985"
     CDS             2524..>3386
                     /gene="glgP"
                     /locus_tag="PK442_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOO87035.1"
                     /translation="MMSLHHQRELGSSIRKLMETDPAFRPVAYLSMEIGIKESLPTYS
                     GGLGILAGDILKSAADLGVPMVALTLLYRKGYFQQSFNEDGWQTEKPVLWQPVQELTL
                     LPNQVSLTLQNREVHVRVWQYEIVGNTGHPLPVYFLDTDFDNNHPDDRKLSWQLYGGD
                     QLYRLCQEMVLGVGGLRMLRDLGYKNIETFHMNEGHAGFLTLELMREQGYFDIEKIRE
                     QVIFTTHTPVPAGHDFFRFDLVDKVISQEALSNLKRMLPNSDGVSMTELGIRFSRYVN
                     AVSKKHAEVSR"
BASE COUNT          757 a         1001 c          911 g          717 t
ORIGIN      
        1 cgcgctttcg ggaagcgccc ctatgagcat cttcgcgaaa tctcctccgt ttctcagggc
       61 atcgccaaag gcaaccttct gggcgagctc cccctggtag aaaaagagcg aaagcgcacg
      121 accggaacgc aggggacaaa tgtatgctcg atgcgtatcg acccgctccc ccgtcacgtc
      181 cgtccatgat gaagcgccct tttcccgaac agcctgcgcc tggcgggggg cgaggatgac
      241 gaagcgtatg ccatgctcgg cgagaatatc aagcgtctcg gaatcgacgg cgcactcggg
      301 tagccacatc ccctctggcg cccgctggaa gcgcttctgg aagtcttcaa tcccccagac
      361 gatctgtgta atcttgtcgc ggcggttcgc aagaggaagg atcatatggt tatacgcctg
      421 ggctatggcg ggaccgtgtc ccgaaaaacg tgccgatccc aagcggtccg cctcgaggac
      481 agcctgatag tactgggggc gttttctctc catccatgaa agaagcgtcg gtccgaaatt
      541 gaaactgatc cgcgaatagt tattgacgat acgccggatc caccccgcgt cgtcaaaaat
      601 acgcgcggcg gagttcggcc cgtagcactc ctcggaaata cgttcattcc agtcatgcca
      661 gggagctgcg gattcttgaa tttccacctc ttctagccag gggttctccc tgggcggctg
      721 gtaaaagtga ccatgcaaac agatcgaaca gggcaagaaa aaccctcctt tcttccggga
      781 taaattatac cctttcttca cctctctttt cgcttggtgt acaataagga gcaaaaaacg
      841 gaggtgtttt ttctgacgag atcttgcggt attctccttc ccgtttcctc tcttccagga
      901 aggtggggca tcggcgacct tggtccggga gccagatcgt ttgttgactt cctctcgcgt
      961 tcgggccaaa aaacatggca gatactcccc ctctccgaaa ccgacggagc gctcggcaac
     1021 tctccgtaca gctcgccttc cgcgtttgcc ggaaatcatc ttttcatctc accggacgac
     1081 ctttttaaat gcggactgct cactgccgcc gatacggcgg aactgcctcc gttcccggga
     1141 gagaaggtcg actatccgac agtccgtgcg gtgaaggaga aacttttagc gcgagcgttc
     1201 gaaaggttcg ctccctcaga ggagtacgaa acctttctac agcagaacga gcattgggtc
     1261 ttcgactatg cgctcttttc cgcattgaaa caacgcttcg gcggtctttc gtggaatcag
     1321 tggcccgacg agctgaaact ccgggacggg aacgctcttc gaaccgccgc aacagagctg
     1381 cgcgaatcag tggacttctt gctcttcaag cagtatctct tcttctcgtc tatggagcgt
     1441 ctgcgcagag agagcgatga aaagggcgtc gaactgctgg gagatctgcc gatctacgtc
     1501 aacttcgaca gcgcggacgt atgggcgcat cccaatcttt ttcacctcga cgaaaatctg
     1561 ctgccggtgg aggttgcggg agttcccccc gactacttca gcgaaacggg ccaactctgg
     1621 ggcaacccgc tgtacaactg ggaagctttg cgggaagacg acttttcatg gtggataaag
     1681 cgcttgcagc actcgctctc catgttccac ctcgtccgga ttgaccattt ccgcggactc
     1741 cttgcctact gggcagtccc tttcggcgac aggaccgctg agaaaggcgt ctggagagat
     1801 gtgccgtcgg aagcgttctt tgcttcactc cgaaagaaca tcccctccgc ttcattcctg
     1861 gccgaaaacc tgggcgtcat tacacccgat gtcacggacg ccatggaacg aatgagcttt
     1921 cccggaatgg cagtcgttct ttttgcgttc ggaggcggga tgaaggacaa tccgcacatt
     1981 ccgcacaact actccgcaac gcttgcagcc tacagcggaa cacacgacaa caacaccgcg
     2041 ctcggctggt accgcgacga cgcctcggag gaagaacgga acacgctggg gcgctatctc
     2101 ggccgaatgc cggacgagaa ggaggttccg gacattatcg taaggatggt actctcctcg
     2161 gttgcacagc gcgcaatcat cccgctccag gactacctgg gacttgggag cgaagcgcgc
     2221 atgaacacgc ccgcgacccc ctcggggaac tgggagtggc gcgctgacga attgtgcttc
     2281 accgaagaac tttcaatcag aataaagcaa ctctccgaaa tttacggaag aaattgatgt
     2341 tcccccttca aagaagaggg ctttttctgg tactattatg aatcaaaggc ttcgtgggtt
     2401 cctttttcgg gcacggcttc cggcggatga tacgcaagat aaatccagag cgtttcttct
     2461 ccggtcgcaa cacgaaagcc ccaacgtttg aatctgtact acttgagagg aggagtcgct
     2521 cccatgatgt cactgcacca tcagagagag cttggcagtt caatccgcaa actaatggaa
     2581 accgacccgg cgtttcgtcc ggtagcatat ctttccatgg agatcggcat aaaagaatct
     2641 ctcccgacct attcgggagg tctgggcatc cttgccggag acatcctgaa aagcgcggcc
     2701 gaccttggcg ttcccatggt cgccctcacg cttctttaca ggaaagggta tttccagcag
     2761 tcgttcaacg aggacggctg gcagacggaa aaacccgtgc tctggcagcc ggtgcaggag
     2821 ttgacgcttc tgcccaacca ggtgtcgctc acgctgcaaa accgcgaggt ccacgtacgg
     2881 gtatggcagt acgagatcgt cggcaacacg ggacatccgc tcccggtcta cttcctggac
     2941 accgatttcg acaacaatca ccccgacgac cgcaaactct cgtggcagct ctacggaggg
     3001 gaccagctct accgcctctg ccaggagatg gtgctcggcg tgggcggtct ccgcatgctc
     3061 cgcgacctcg ggtacaaaaa catcgagacg ttccacatga acgagggaca cgccggtttc
     3121 ctcaccctcg aactcatgag ggaacaaggc tacttcgaca tcgaaaagat ccgtgaacag
     3181 gtcatcttca cgacgcatac cccggttccg gcagggcacg acttcttccg tttcgacctt
     3241 gtggacaagg tcatctcgca agaggcgctc tccaatctca agaggatgct cccgaactcg
     3301 gacggcgttt ccatgaccga actgggcatc cgtttcagcc gctacgtcaa cgccgtctcg
     3361 aaaaaacacg ccgaggtcag ccggaa
//
LOCUS       DAOSWW010000187         3382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20141_length_3382_cov_3.736700, whole genome shotgun sequence.
ACCESSION   DAOSWW010000187 DAOSWW010000000
VERSION     DAOSWW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20141_length_3382_cov_3.736700"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PK442_04990"
     CDS             <1..957
                     /locus_tag="PK442_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO87036.1"
                     /translation="SRSGVPLRGLAEALNAPVLLTCAGKGVLSEEHPLCLGARLHFPA
                     VRELLAKADCLLALGTEISPTDLWTDEFEFSGTVVSVDNDEGHRASRNGVFLHGRCEE
                     VLPLLLAGVAKKGAARWAEKDALLARCSQELGATLGVADELPLLAETTRALRTSLGDD
                     GTLWADMTGPAYYAISEYAAALPGRFLHPVGFGTLGAALPAALATKCAFPERRVAALA
                     GDGGFQFTLPELAVGVQEKLSLPVILWNDGGFGEIRRCQQAKGGPLIAVDHWNPDFRA
                     LAASYGIPYFAPEEGALGDALEEAFAAPVPSLIEIRARKECC"
     gene            957..2072
                     /gene="hisC"
                     /locus_tag="PK442_04995"
     CDS             957..2072
                     /gene="hisC"
                     /locus_tag="PK442_04995"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOO87037.1"
                     /translation="MIAGVRKAVGAATPYVPGKTVEEVRRELGLEKIVKLGSNENPYG
                     PFPAARRAMTEEIGRLNTYPDVSFREIKSLIGERFGLGPEWVAISHGAEGMLQTLGKV
                     FLEPGDEAILPCATYGLYREISLVMGATLKEIMLKDDLSVNLREMERAITGRTKLVWL
                     NNPNNPTGMLLPKKSVETLIKRLPRGVWLVLDEAYAEFASPEELPSVAKLLEAECSLI
                     VVRTFSKAWGLAGARIGYALARPEMVRIIDTVSEPFNANRIAIAGASASLKDDEPAFN
                     QALAATVRERERVTKALRDMGGETLPSHTNFVFVTFQTDAGEISEKLLRKGVIVRPCG
                     GWGFGCSLRVTIGTPEQNDFFLEALRGVLSGDGEGGA"
     gene            2069..3259
                     /locus_tag="PK442_05000"
     CDS             2069..3259
                     /locus_tag="PK442_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163919.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HOO87038.1"
                     /translation="MSTERIASGDGVVFTDVCKIYPDGTRAVDMLNLDIRRGELVSLI
                     GPSGCGKTTTLKMINRLEEPTSGSIVVGGEDVRSSDAVALRRRIGYVVQEIALFPHLT
                     VAENINVVPELLGWSAAKMRSRADELLEMAGLAPERYRYRLPDQLSGGQKQRIGVLRA
                     LAADPQVILMDEPFGALDPISRETLQNELVSLQKNLRKTIVFVTHDMDEAIRISDRIA
                     ILRKGRVEQIGTPEEIQTAPGNDFIRNFIGEDRLSQMSPDDSVGMLLEEPWAVAAPSE
                     SASAVLERMEENDREFIHVVDPKRGVWLGAALLGRVRRAAVAGKSVVESLSRDRKLYL
                     EDAVLRDVAEMLADRDISIPVLDSGERLLGVITSAGLARLAMNRLRGNRTARAAGEKK
                     EGTR"
BASE COUNT          605 a         1043 c         1140 g          594 t
ORIGIN      
        1 tcccgctccg gcgtcccgct tcgcggactg gccgaggctc tgaacgctcc ggtcctgctc
       61 acgtgcgcgg gaaagggcgt tctctccgaa gagcatccgc tctgcctcgg cgcgcgcctt
      121 cactttcccg cggtcaggga gctgcttgcg aaagccgact gcctcctcgc gctcggtacc
      181 gagatctccc cgaccgatct ctggacggat gagttcgagt tctccggaac tgtggtctct
      241 gtggataatg acgaggggca tcgcgcctcc cgcaacggag tgttcctgca cggaaggtgc
      301 gaggaggtgc ttccgctgct gctggccgga gtcgccaaaa agggcgccgc gcggtgggcg
      361 gagaaggacg cgcttcttgc ccgctgctcg caggagctgg gcgcaacgct tggcgtcgcg
      421 gacgagttgc cgctgctcgc agagacgacg agggcgctca ggacctcgct cggcgacgac
      481 ggaacgctct gggcggatat gacgggcccg gcgtactacg cgatcagcga gtacgccgcc
      541 gcgctccccg ggcgtttcct ccacccggtc ggcttcggga cgctcggcgc ggcgttgccc
      601 gccgcgttgg cgacgaagtg cgcctttccg gaacgccgcg tcgccgcgct cgcaggcgac
      661 ggaggttttc agttcacgct tccggagctc gcggtgggtg tccaggaaaa actgtcgctc
      721 ccggtgatct tgtggaacga cggcggcttc ggcgagatca ggcgctgtca gcaggcgaaa
      781 ggcggtccgc tcatcgccgt cgatcactgg aaccccgatt ttcgcgcgtt ggcggcatct
      841 tatggaatcc cctatttcgc gcctgaagaa ggcgcgctcg gcgacgcgct cgaagaggcg
      901 ttcgcagccc ccgtcccgtc gttgatcgaa atccgcgccc gaaaggagtg ttgctgatga
      961 ttgcaggagt cagaaaagcc gttggagcgg cgaccccgta tgttccgggg aagaccgtcg
     1021 aagaggtccg cagagagctg gggctcgaaa agatcgtgaa gcttggctcg aacgagaacc
     1081 cgtacggccc cttccccgca gcccgcaggg caatgacgga ggagataggg cggctcaaca
     1141 cctacccgga cgtctccttc cgcgaaatca agagtctcat cggcgagcgc ttcgggctcg
     1201 gcccggagtg ggtggcgatc tcgcacggcg ccgaagggat gctccagacg ctcggcaaag
     1261 tctttctgga gcccggcgac gaggctattc ttccgtgcgc cacttacgga ctctaccggg
     1321 agatctcgct cgttatgggg gcgacgctga aagagatcat gctcaaggac gatctctccg
     1381 tcaatctgcg ggagatggaa cgcgcgatca ccgggcgcac caagctggtg tggctgaaca
     1441 acccgaacaa ccccaccggg atgctgctcc ccaaaaagag cgtcgagacg ctgataaagc
     1501 gccttccccg cggggtctgg ctcgtcctcg acgaagcgta cgccgagttt gcctccccgg
     1561 aagagctgcc ttcggtcgcg aagctgcttg aggcagagtg cagcctgatc gtcgtgcgga
     1621 ctttttcaaa ggcgtgggga ctcgccgggg cgcgtatcgg gtatgcgctg gcgcgtccgg
     1681 agatggtgcg gatcatcgac acggtgagcg agccgttcaa cgccaacagg atcgccatcg
     1741 ccggcgcctc ggcgtcgctg aaggatgacg aacccgcgtt caaccaggcg ctcgcggcga
     1801 cggtgcggga gcgggagcgg gtaaccaagg cgcttcgcga tatgggaggc gaaacgcttc
     1861 cttcgcacac caactttgtc ttcgtcacct tccagacgga cgcgggcgag atctcggaaa
     1921 aactgctccg caagggcgtc atcgtgcgcc cctgcggcgg ctgggggttc ggttgttcgc
     1981 tccgcgtgac gatcggcacg ccggaacaga acgacttctt ccttgaggcg cttcgcggcg
     2041 ttctttcggg agacggggag ggaggagcat gagcacggag cgaatagctt caggagacgg
     2101 cgtcgtcttc acggatgtct gcaagatcta ccctgacgga acgagggcgg tcgacatgct
     2161 gaacctcgac attcgcaggg gggagctggt cagcctcatc ggtccgtcgg ggtgcggcaa
     2221 gacgacgacg cttaagatga tcaaccgcct cgaagaaccg acgtccggga gcatcgtcgt
     2281 cggcggggag gatgtccggt cgagcgatgc tgtggcgctc cgtcgccgta tcgggtacgt
     2341 ggtccaggag atcgcgctct ttccgcacct caccgtggcg gagaatatca acgtcgttcc
     2401 cgagctgctc ggatggtctg cggcgaaaat gcggagcagg gcggacgaac tgcttgagat
     2461 ggccggactt gcgccggaac gctaccgcta ccgtctcccc gaccagctga gcggcgggca
     2521 gaagcagcgc atcggcgttc tccgcgcgct ggcggccgat cctcaggtca tcctgatgga
     2581 cgagccgttc ggagcgctcg atccgatcag ccgtgagacg ctgcagaacg agctggtatc
     2641 gctgcagaaa aatctccgga agaccatcgt cttcgtcacc cacgacatgg acgaggcgat
     2701 ccgtatctcc gaccgcatcg ccattctgcg gaaagggaga gtcgagcaga taggaacgcc
     2761 cgaggagatt cagacggcgc ccgggaacga cttcatacgg aacttcatcg gcgaggacag
     2821 gctctctcag atgtcgcccg acgactccgt cgggatgctt cttgaagagc cgtgggctgt
     2881 cgctgcgccg tcggaaagcg cttcggccgt gctcgagcgg atggaggaga acgaccgtga
     2941 gttcatccac gtcgtcgatc cgaagcgcgg cgtctggctc ggcgccgcgc tgctcgggcg
     3001 ggtgcgccga gccgcggtcg cgggcaagag cgttgtcgaa agcctctccc gcgacaggaa
     3061 gctctacttg gaggacgcgg tgttgcgcga cgttgcggag atgctcgccg acagggatat
     3121 ctccattccc gtcctcgatt ccggggagcg gctcctgggc gtcattacat cggccgggct
     3181 cgcccgtctt gccatgaacc gtcttcgcgg aaacagaacg gcccgtgcag ccggagagaa
     3241 gaaggaggga acgcgatgag ccaaaagagc ctgatcgacc tgttcatcac cttcgtcggg
     3301 aggaactggc agcggatact ctggctcacc ggagagcacc tgctcatctc tctgatcgcc
     3361 gtcggcatca ccgttaccgt ct
//
LOCUS       DAOSWW010000188         3370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20243_length_3370_cov_3.369231, whole genome shotgun sequence.
ACCESSION   DAOSWW010000188 DAOSWW010000000
VERSION     DAOSWW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20243_length_3370_cov_3.369231"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /gene="ygfK"
                     /locus_tag="PK442_05005"
     CDS             complement(<1..1905)
                     /gene="ygfK"
                     /locus_tag="PK442_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOO87039.1"
                     /translation="MSDIMRMIPFGNLMDWMLVEHGKEGSIFGIRKNKFYKNATGKQI
                     VVCGESISSPIGPAAGPNSQLAQNIIAAYLAGSRFIELKTVQIMDGEELRKAVAKPCI
                     NAQDEGYNVEWSTELTVQEAFDEYVKAWFAIQVMAKELGISAQRDFAYNMSVGYDLKG
                     IQSPKIDGFIEGLKNASGSGIWKTCSQWLRDNLSKFSNVNASDVDAIESRLCSSITLS
                     TLHGCPPEEIERISRYLFEEKGLHVFVKCNPTLLGYETARDLLDRMGYTYIDFTDHHF
                     KHDLQYSDGVAMFKRLQEFAKERGLEFGLKLTNTFPVNIKNEELPGEQMYMSGRSLFP
                     LTITLASKLSREFGGKLQISYSGGADFFNIDSILNVGIQPISMATTILKPGGYERMKQ
                     IAEKAEPYLNGAFRGVDVKALDALAASVFEKRFHKKSARPVGSRKTESRLPLFDCAKA
                     PCQDGGCPINQQVPTYLRLVNEKKYAEAFDVIAMDNSSPAVTGTICDHQCQHVCTRLD
                     YEQPLEIREMKKIAVLNAQDEYIARMQPAKIVSKKKAVVVGAGPAGVGTAFFLRRNGI
                     ETTVLERRAEPYGIVKYVIPEFRIGEDMIARDVALAKKAGVQFVFGVDGKIDIEALKK
                     RYEIDVYESEE"
     gene            complement(1942..>3370)
                     /locus_tag="PK442_05010"
     CDS             complement(1942..>3370)
                     /locus_tag="PK442_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO87040.1"
                     /translation="RVRIKTGVKNDGSITALDMSDVMNTGAYGAHALTVLSNAGSKVL
                     PMFNKIENMNFVGRAVYTNLPIGGAYRGYGATQGYFAFNQHMDGIARRLGVDFLDFVK
                     KHHIREGETSEVFKAIGEGTEGVSQIIRSCKLSECIDKGAEAIGWSSLRGKKIANGPV
                     VKGIGAAVSMQGSGIPQVDMASASMKMNDDGSFNLYVGATDIGTGSDTILSQIAAETL
                     NVPLEMILILSSDTDLTPFDTGAYASSTTYVSGQAVLRCAEKVRSQILSVAEKMTGAA
                     PESLVFKESRVVNTATGEETSFSDIACFAMYSHDQFQIQASASFTGSESPPPFMAQFA
                     EVDVDTETGKVRVVKFVSVGDCGQPLNPKLVEGQIEGATMNGISYALTEQYIFNSKGK
                     MTNNSFWDYKLFGTLDMPEMKTIVVDSDEPTGPYGAKSISEIGINGPAPAIANAIYDA
                     VGVRIYDLPLTPEKVLKAIRARTDG"
BASE COUNT          688 a          983 c          892 g          807 t
ORIGIN      
        1 ctcttcggac tcatagacat ctatttcata acgcttcttc agcgcttcta tatctatttt
       61 tccgtcgacc ccgaagacga actgtactcc agccttcttc gcgagagcga cgtctcgggc
      121 aatcatgtcc tcgcctatac ggaactctgg aatgacatac ttcacgatgc cgtacggttc
      181 ggcccggcgc tcaagcaccg tcgtctcgat gccgttccgc cgaaggaaaa acgctgtccc
      241 cactccggca ggaccggcgc cgacaacaac cgcctttttc ttcgagacga tttttgcagg
      301 ttgcatccgg gcaatatact catcttgcgc gttgagcacg gcaatcttct tcatctcgcg
      361 tatctcaagc ggctgttcgt agtcgagccg ggtacagacg tgctggcact gatggtcgca
      421 gattgttccc gtgactgccg gcgaactgtt gtccatcgcg atgacatcga acgcttcggc
      481 gtacttcttc tcgttgacca gacggagata cgtcggaacc tgctggttaa tggggcaccc
      541 gccgtcctgg cagggggcct tggcgcagtc gaaaagcgga agacgcgatt cggtcttcct
      601 tgatccaaca gggcgggcgc tttttttgtg gaagcgcttt tcaaagacgc tcgccgcgag
      661 ggcgtcaaga gccttcacgt ccactccgcg gaacgccccg ttcaggtacg gctccgcttt
      721 ctcggctatc tgcttcattc tttcgtatcc gccgggcttg agaattgtag tggccatgct
      781 gatcggctga ataccgacat tcagtatgga gtcgatatta aagaagtcgg cgccgcctga
      841 gtacgagatc tggagctttc cgccgaattc gcggctcagc ttgctcgcca aggtaatcgt
      901 cagggggaaa agcgaacggc cggacatgta catctgttcg ccgggcagct cttcattttt
      961 aatattcacg gggaaggtgt tggtaagctt cagaccgaac tcgagtcccc gctccttcgc
     1021 gaactcctga agcctcttga acatcgcaac gccgtcgctg tactgaaggt catgcttgaa
     1081 gtgatggtcc gtgaagtcaa tataggtata ccccatccgg tcaagaagat ctcgggccgt
     1141 ttcatacccc agaagcgtcg gattgcactt cacaaagacg tggagcccct tctcctcgaa
     1201 cagataccgt gaaatacgtt cgatctcctc cggcgggcag ccgtgaagcg tagaaagcgt
     1261 tatcgacgaa cataaccggc tttcgatagc atccacatcc gaagcattga cgttgctgaa
     1321 cttcgaaagg ttgtcccgca gccactggga acacgtcttc cagatgccgc tcccggaggc
     1381 gttcttcaac ccttcgatga acccgtcgat cttgggggac tgaattccct tcaaatcgta
     1441 gccgacgctc atattgtagg cgaaatcgcg ttgtgcgctg attccaagct cctttgccat
     1501 cacctggatt gcgaaccagg ccttgacata ttcgtcgaac gcttcctgga cagtgagttc
     1561 cgtagaccat tccacgttgt acccttcgtc ctgagcgttg atacagggct tggctaccgc
     1621 cttccgaagt tcttctccgt ccatgatctg cacggttttc agctcgataa agcgtgaacc
     1681 ggccagatag gctgcaatga tattctgcgc aagctgggag ttaggaccgg ctgcagggcc
     1741 gatcggcgag ctgatggatt cgccgcagac cacgatctgc tttcccgtag cgttcttata
     1801 gaatttgttt ttacggatac cgaagatgga gccttccttg ccgtgttcga ccagcatcca
     1861 gtccatcagg tttccgaaag gaatcattct cataatgtcg ctcattcctg cacctccgca
     1921 atcataatcg ctggattttg cttacccatc cgtacgcgcg cgaatcgcct tgagcacctt
     1981 ctcgggcgtc aggggaagat cgtaaatccg gacgccgacg gcgtcataaa tcgcgttcgc
     2041 gatagccgga gccgggccgt tgataccgat ttcagaaatg gacttcgccc cgtacggccc
     2101 cgtcggctcg tcggaatcga ccacaatggt cttcatctca ggcatatcca gcgtgccgaa
     2161 cagtttgtaa tcccagaagg agttattggt catcttcccc ttcgagttga aaatgtactg
     2221 ttcagtcagc gcgtagctga tcccgttcat tgtggcgccc tcgatctgcc cttcgacaag
     2281 cttggggttg agcggctggc cgcagtctcc cacggacacg aacttgacaa cgcgcacctt
     2341 tcctgtttcg gtatcgacgt cgacctcggc gaactgcgcc atgaaaggag ggggcgactc
     2401 cgagcctgtg aacgacgccg aagcctgaat ctggaactgg tcatgactgt acatggcaaa
     2461 gcaggcaata tcggagaagg acgtctcttc gcctgttgca gtattgacca cgcggctctc
     2521 cttgaataca agactctcgg gagcagcgcc cgtcatcttt tccgcaacgg agagaatctg
     2581 cgaacgcacc ttctctgcgc atcggaggac cgcctgcccg gagacgtacg tggtggacga
     2641 agcgtaggct ccggtatcga acggcgtcag atccgtatcg gaggagagaa tcaggatcat
     2701 ttcaagcggc acgttcaaag tttccgccgc gatctgggag agaatcgtat ccgaacccgt
     2761 gccgatatcg gtggcgccga cgtagaggtt gaacgaaccg tcgtcgttca tcttcatcga
     2821 ggcgcttgcc atatcaacct gaggaatgcc cgagccctgc atggatactg ccgcgccgat
     2881 tcccttcacc acgggaccgt ttgctatctt tttgccgcga agactgctcc agccgatcgc
     2941 ttccgctccc ttgtcgatgc actcgctcaa cttacacgac cggattatct ggcttacacc
     3001 ttctgtgcct tcgccgatgg ccttgaagac ttcggatgtt tcgccttccc ggatgtggtg
     3061 cttcttaacg aaatcgagaa aatcaacgcc gagcctccgg gcgattccat ccatgtgctg
     3121 gttgaaggcg aagtagccct gggttgcccc gtagccgcga taggcgcctc cgatggggag
     3181 attcgtgtag accgctcttc ccacgaaatt catattttcg atcttattga acatcgggag
     3241 caccttggaa ccggcgtttg agagcaccgt cagcgcgtgc gctccgtatg ccccggtgtt
     3301 catcacgtcg ctcatatcga gcgcggtgat cgatccgtcg ttctttacgc cggtcttgat
     3361 gcgaacgcgc
//
LOCUS       DAOSWW010000189         3354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20370_length_3354_cov_4.151258, whole genome shotgun sequence.
ACCESSION   DAOSWW010000189 DAOSWW010000000
VERSION     DAOSWW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20370_length_3354_cov_4.151258"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PK442_05015"
     CDS             complement(<1..1344)
                     /locus_tag="PK442_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOO87041.1"
                     /translation="MKKQEIGSFIEKKRDVFIELSDKIWAFAETAFVEFQSADLLCEA
                     LEKEGFSVERGTGGVATAFCGSWGSGSPVIAFLGEYDALSGLSQKGGVALKEPLVPGG
                     NGHGCGHNNLGAGAFAAAVAFREYLKSSGKSGTVRYYGCPGEEGGSGKAFMARAGVFD
                     DVDAALTWHPGSVNAVFPFSSLANFQVAYRFKGKSAHAAACPHLGRSALDAVELMNVG
                     VNYLREHVIPEARMHYAITDSGGFSPNVVQPKAEALYLLRAPRTPQVEEIYERVNKIA
                     RGAALMTETELEIRFIKACANVVQNNVLERVLERNFAELGVPEYTEEEISFARAIFDG
                     IAPSERSSDLDQYSAPGGAAGKESAKRLRERPIADEVLPYGESDFVLPGSTDVGDVSW
                     IVPTCQAWVATWPNHTPGHSWQIVSSGVTPLAHKGMLHAGKILAAAAADLFDDPSL"
     gene            complement(1364..1765)
                     /locus_tag="PK442_05020"
     CDS             complement(1364..1765)
                     /locus_tag="PK442_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOO87042.1"
                     /translation="MKPTALSSSRAPAPKGFYSQAVRVGSLLFTAGQLPVDPESGDFV
                     RGTVEAQTERVIENIRGILEENGSSLSLVVKATVYIRDIASWGSVNSVYSRYFEGTVP
                     PARTVVGGVDIHYGLDVEMDVVAYVEETGQE"
     gene            complement(1941..2972)
                     /gene="lsrB"
                     /locus_tag="PK442_05025"
     CDS             complement(1941..2972)
                     /gene="lsrB"
                     /locus_tag="PK442_05025"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF011937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HOO87043.1"
                     /translation="MKKFAVALAVVMLLVGAAAAVAAPKDVSVVFIPKLTGNMFFESA
                     AEGALAMAKEVGFECKYDGSPEASVANQVQIINSAVNQGFNAISVSSLSPTGLSQALQ
                     NAKKRGVKVVTWDSDVDPKDRAIYVNQGTPEILGAMLVDMAYDQMNDEQKKSAKFAFF
                     YSSPTVTDQNAWVDFAKKYIAEKHPGWEMLTVQYGEQDAQKSLQIGQSILQTYPDIDA
                     IICPDSTALPAMAQAAKNLEKAGKVIITGFATPSSMRGFVKDDTVKAFGLWDCKLQGA
                     IGAYIAYWLADGNTFKVGDFIDVPGVGKLKVEPNSIQGYDYEAEDSGIVLLPERVVFT
                     KENIDNYNF"
     gene            complement(3084..>3354)
                     /locus_tag="PK442_05030"
     CDS             complement(3084..>3354)
                     /locus_tag="PK442_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87044.1"
                     /translation="PIVTAVVLGGVSITGGRGTVVGVAIAGVVVGLLRFGLQMAGVSS
                     QYITVGTGMLLVVSAAVRGLAFRFSLLPRRSGGGDNIKGGGARRG"
BASE COUNT          560 a         1131 c         1055 g          608 t
ORIGIN      
        1 cagcgatggg tcgtcgaaca ggtcggcggc cgccgccgcg aggatcttcc ccgcgtggag
       61 catccccttg tgcgcaagcg gggtgacgcc cgaggagacg atctgccacg agtgccccgg
      121 agtgtggttc ggccacgtcg ccacccaggc ctggcaggtg ggaacgatcc agctcacgtc
      181 gcccacgtcg gtcgatccgg gaaggacgaa gtcgctctct ccgtagggaa gcacctcgtc
      241 ggcgatcgga cgttccctga ggcgtttcgc cgactccttg cccgccgctc cccccggcgc
      301 ggagtactgg tcgaggtcgg agctgcgctc ggagggcgcg atcccgtcga agatcgcccg
      361 ggcgaaggag atctcctcct ccgtgtactc cggcacgccg agctcggcga agttccgctc
      421 gagtacgcgt tcgagcacat tgttctgcac cacgttggcg cacgccttga tgaagcggat
      481 ctcaagctcc gtctccgtca tcagggccgc gccgcgggcg atcttgttca cccgctcgta
      541 gatctcctca acctgcggcg tgcgcggggc gcggagcagg taaagcgcct cggccttcgg
      601 ctgcaccacg ttgggggaga agcccccgga gtcggtgatg gcgtagtgca tccgcgcctc
      661 ggggatgacg tgctcgcgca ggtagttcac acccacgttc atcagctcca ccgcgtcgag
      721 cgcgctccgc ccgaggtgcg ggcaggcggc ggcgtgggcg ctcttcccct tgaaccggta
      781 ggcgacctgg aagttggcca gcgagctgaa ggggaagacc gcgttcacag agccggggtg
      841 ccacgtaagc gcggcgtcca cgtcgtcgaa gacgcccgcc ctcgccatga aggccttccc
      901 ggagccgccc tcctcgccgg ggcagccgta gtagcgcacc gtgccgctct ttccgctgct
      961 cttcaggtac tccctgaagg ccacggccgc ggcaaaggcc cccgcgccca ggttgttgtg
     1021 gccgcatccg tggccgttcc cgccggggac cagcggctcc ttcaatgcga cgccgccctt
     1081 ctggctcagc ccggagagcg cgtcgtactc gcccaaaaag gcgatcaccg gcgagccgct
     1141 cccccacgag ccgcagaaag cagtcgccac gccgccggtc ccgcgctcca cggagaaccc
     1201 ctccttttcg agggcttcgc agagtaaatc cgcggactga aattccacga acgccgtctc
     1261 ggcgaacgcc catatcttgt cgctcagttc gatgaatacg tcccgttttt tctcgatgaa
     1321 acttccgatt tcctgcttct tcatacccgg ttcctcccct ctgctactcc tgccccgtct
     1381 cctccacgta cgccacgacg tccatctcca catcgagccc gtagtggatg tccacgccgc
     1441 cgaccactgt tctggcggga ggcacggttc cctcgaaata gcggctgtac acgctgttca
     1501 cgctccccca cgaagctata tcgcggatgt agaccgtagc cttgactacg agcgacaggg
     1561 aggagccgtt ctcctccaga atcccgcgga tgttctcgat gacgcgttcg gtctgcgctt
     1621 ccaccgtgcc gcggacgaag tcgccgcttt ccgggtccac agggagctgc cccgcggtga
     1681 agaggagcga tccgacgcgg acggcctgcg agtagaaccc cttgggggcc ggcgcccgcg
     1741 acgaggagag tgctgtaggc ttcattccgt attccctccc gagagaaaaa tatacttgta
     1801 ccaattgagt atgacatttc cggagacagg acgcaagagc agacggaaag gcggcggccc
     1861 gggagcgctt ggggcttgcc gggtatgaaa aacgcgcccc cgaggcgccg ggagcgcgtg
     1921 gcgaagccgt gcagcgatgg ttagaagttg tagttgtcga tgttctcttt ggtgaagacg
     1981 acccgctcgg ggagcaggac gatgccggag tcctcggcct cgtagtcgta cccctggatg
     2041 gagttgggct cgaccttcag cttgcccacg ccggggacgt cgatgaagtc gccgaccttg
     2101 aaggtgttgc cgtcggccag ccagtaggcg atgtacgcgc cgatcgcgcc ctggagcttg
     2161 cagtcccaca ggccgaacgc cttgacggtg tcgtccttca cgaagccgcg catcgagctg
     2221 ggggtggcga atccggtgat gatgaccttt ccggccttct caaggttctt ggcggcctgc
     2281 gccatcgcgg gaagagcggt ggagtcgggg cagatgatcg cgtcgatgtc ggggtaggtc
     2341 tgaaggatgg actggccgat ctgaagggac ttctgcgcgt cctgctcgcc gtactgaact
     2401 gtcagcatct cccagccggg gtgcttctcg gcgatgtact tcttcgcgaa gtcgacccat
     2461 gcgttctggt cggtgacggt cgggctggag tagaagaagg cgaacttcgc gctcttcttc
     2521 tgctcgtcgt tcatctggtc ataggccatg tcgacgagca tcgcgccgag gatctcgggc
     2581 gtcccctggt tgacgtagat ggcgcggtcc ttggggtcga cgtcggaatc ccacgtcacg
     2641 accttgacgc cgcgcttctt cgcgttctgc agcgcctggc tcagccccgt gggggagagc
     2701 gaggagacgg agatggcgtt gaagccctgg ttcacggcgc tgttgatgat ctgcacctgg
     2761 ttggcgacgg acgcctcggg ggagccgtcg tacttgcact cgaagccgac ttccttggcc
     2821 atggccagcg cgccctcggc cgccgactcg aagaacatgt ttccggtgag cttgggaata
     2881 aagacaacgc tgacatcctt cggcgccgca accgcagccg ccgcaccgac cagaagcatc
     2941 actaccgcaa gcgctaccgc aaatttcttc attacaagtc cctcctcttt tcttttcggg
     3001 gccgcaggcc ccggttcatt cccctcctga tccggcgacg ctccgccgaa ccgctcaatc
     3061 caccgcgcat caaccaatcc gcatcagccg cgccgggcgc cacctccctt gatattgtcc
     3121 cctccgccgg aacggcgggg aagcagtgaa aaccggaagg cgagccctct gaccgccgcc
     3181 gagacgacga gcagcattcc cgtccccacg gtgatgtact ggctggacac cccggccatc
     3241 tgcaatccga agcgcagcag ccccaccacg acgcccgcga tggccacgcc gacgaccgtc
     3301 cccctgcccc ccgtgatgga gacgccgccg aggacgaccg cggtaacgat gggc
//
LOCUS       DAOSWW010000190         3352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20392_length_3352_cov_5.586594, whole genome shotgun sequence.
ACCESSION   DAOSWW010000190 DAOSWW010000000
VERSION     DAOSWW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20392_length_3352_cov_5.586594"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PK442_05035"
     CDS             complement(<1..309)
                     /locus_tag="PK442_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87045.1"
                     /translation="MNEKNDLRSVIATLAGVLGSPYFSKGDLAELRRMTCDSPPLPFW
                     RALYDYVPESLRMNEAMENHWITILNGMAVMAPGIHSDAPSHSVGATFSLFPIQRMNH
                     F"
     gene            505..996
                     /locus_tag="PK442_05040"
     CDS             505..996
                     /locus_tag="PK442_05040"
                     /inference="COORDINATES: protein motif:HMM:NF040103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HOO87046.1"
                     /translation="MPESCFFCEGPFRFHGFRKRRCILLGEKRPFFVKRFRCCVCRKT
                     FTVLKKFMLPFKQHAASEIEEALSGEKESGTHADERTIGRWRKAFHTVLPALCALLED
                     LAEAVFGSKVSLSASPESPLKRLRRASETLGTYLPGESALARAMFLESRHRLCLGWQS
                     FRL"
     gene            1054..2454
                     /locus_tag="PK442_05045"
     CDS             1054..2454
                     /locus_tag="PK442_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOO87047.1"
                     /translation="MKEIELDGDREEGLRRFALVAPLLEEGLSSSEATERRLRILERE
                     EISERTLRRWVAAYRSGGFEGLVKKPRKDKGTCRAISPEALALAEECRRELPRRSAGL
                     IRDWLGTQGHEVARSTLERHLRANGLSGRDLRAEREAKSGSRRFVRIGRNSLWQADLK
                     YGPYIPDPEHPGRKRRTYLLAILDDATRKVTHAEFYDNQKLPVLEDAMKKAIQRHGSP
                     KRIYVDNGKIFVSTWMKLACAKLNIKHINASPYNPEGKGKIERFNRTVEEFLAEYALQ
                     EAGTLEGLNSFFRSWLSERYQHKAHGALNGKTPAEMFAEDATPLRFHSLETLRDAFLH
                     EGERTVDKSGCLKLEGDLYDAGTDYLRKKVTIRYDPFDREIVQLWYKGTRRSDIRKAR
                     IGESNATLKAPAEKLDAGGESRVLNTYREEHRKRFVKTLGAYGLRNAGKSGESRAGEH
                     AAGEERSGSGEEKLRD"
     gene            2447..3247
                     /locus_tag="PK442_05050"
     CDS             2447..3247
                     /locus_tag="PK442_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO87048.1"
                     /translation="MIESFFGLHGIPFGRSVPVAELLRTDGWDELHARMLHTARARGF
                     GVFTGDTGTGKTTSLRRFAGELDPNRYRVLYVCDSFLTPRNFYWEVLHQLGCTPRFYR
                     GDAKRQFQKALREVNEERKNPVVVVDEAHLLSREMLEEIRFLLNTGMDSESSLALLLV
                     GQSELKDALKLQVNQAIAGRVDIRFHLGAMDKGQTASYVKRHMDSVKAPRDIFTSTAL
                     GMIHEYAGGIPRKVNKVASSCLMTAAVRKETLVDDHLVQEVLESEFEI"
BASE COUNT          734 a          906 c         1104 g          608 t
ORIGIN      
        1 gaaatggttc atgcgttgga tcggaaacag ggagaatgtt gctcccacag agtgcgacgg
       61 agcgtcggaa tggattcccg gggccatgac ggccataccg ttgaggatcg ttatccagtg
      121 gttttccatg gcttcgttca tacgtagaga ttccgggacg tagtcgtaga gcgctctcca
      181 gaaaggaagc ggcggagagt cgcatgtcat gcgtctgagt tccgctaggt cgcctttgga
      241 gaaatacggg ctgcccagta ctcctgcaag ggtagcgatt acgctccgga ggtcgttttt
      301 ttcgttcatc gtcatatcct gtttcggccc ggtcttttcg accggaatag gctttacatg
      361 ggagtccgtc cggagtaatc tgtatacaca ggctgaagag aaaggagagt cccccggacg
      421 acctctccgc caagacagga tcgaaccggg ggactatcgt gaaaaaccat tcgaattata
      481 ccagaagaca acgaagattt tcccctgccc gagtcctgtt ttttctgcga aggcccgttt
      541 cgtttccacg gcttccgcaa gcgacgctgc attcttctcg gagagaagcg tccgttcttc
      601 gtgaagcgct tccgttgctg cgtctgccgg aagacgttca ccgtcctcaa gaagttcatg
      661 ctccccttca agcagcatgc cgcttccgag atcgaagagg ccctttccgg agaaaaggag
      721 agcgggacgc atgcggacga gcgcaccatc ggacgttgga ggaaggcttt tcacacggtt
      781 cttcccgctc tttgcgccct tttggaggat ctcgcggaag cggtgttcgg ttcgaaggtt
      841 tccctgtccg cgtcgccgga atctccattg aagcgtctgc gccgcgcgtc ggagacgctg
      901 gggacgtatc ttcccggtga atccgctctg gcgcgggcga tgtttctcga atcccgccac
      961 cgactttgcc ttggatggca atccttccgc ctgtagactc cgttccgtgc tcccggacga
     1021 ggagaaaaaa cggagaggaa ggacgtggcg gggatgaagg aaatcgaact ggacggcgac
     1081 agagaggaag ggctccggag attcgctctg gtggcgccgt tgctggagga ggggttgtcg
     1141 tcttcggaag ccacggagcg gcgtctgagg attctcgagc gcgaggagat ttcggagcgg
     1201 acgttgagga gatgggtcgc ggcgtaccgg agcgggggct tcgaagggct ggtgaagaag
     1261 ccgcgcaagg acaaggggac gtgcagagcc atatcgccgg aggcgctcgc tctcgcggag
     1321 gaatgccgca gggaacttcc ccggcggagc gcgggtctta tccgcgactg gctgggtacg
     1381 caggggcacg aggtcgcccg gtcgacgctg gagcggcatc ttcgggccaa cggactctcc
     1441 ggaagagacc tgcgcgcgga gagggaggcg aaatcgggga gcaggcgttt cgtcaggatc
     1501 ggaagaaaca gtctgtggca ggcggatttg aaatacggac cgtacatccc cgacccggag
     1561 catcccggaa gaaagcggcg aacgtatctt ctggccatcc tcgacgacgc caccaggaag
     1621 gtgacgcacg cggagttcta cgacaaccag aaacttcccg tgttggagga cgcgatgaag
     1681 aaggcgatcc agagacacgg ctcgccgaag cgcatctacg tcgacaacgg aaagatcttc
     1741 gtctcgacgt ggatgaagct ggcgtgcgcg aagctgaaca taaagcacat caacgcctcg
     1801 ccgtacaatc cggagggaaa gggcaaaatt gaacgtttca acaggacggt cgaggagttt
     1861 ctggcggaat acgcgcttca ggaggcgggg acgctcgagg ggctgaattc gttcttccgt
     1921 tcctggctgt cggaacgcta ccagcacaag gcgcacggtg cattgaacgg aaagacgccc
     1981 gcggagatgt tcgcggagga cgcgacgccg cttcgattcc acagcttgga gacgctgcgg
     2041 gacgcatttc tccacgaggg cgagcggacg gtggacaaaa gcggctgcct gaagctggaa
     2101 ggcgatctct acgacgccgg gacggactat ctgcgcaaga aggtgacgat ccgctacgac
     2161 ccgttcgaca gggagatcgt ccagctctgg tacaagggaa cgcggcggag cgacatccgg
     2221 aaggcccgca tcggagagag caacgcgacg ctgaaggcac ccgccgagaa gctggacgcg
     2281 ggaggagaat cccgtgtgct gaacacctac agggaggagc accgcaagcg tttcgtgaag
     2341 acgctcggtg cgtacggcct gaggaacgcc gggaaaagcg gggaaagccg cgcaggggaa
     2401 catgccgccg gagaagagcg ctccggaagc ggagaggaga agctccgtga ttgagtcgtt
     2461 cttcggactt catggaatcc ccttcggccg cagcgttccc gtcgccgaac tcctgcggac
     2521 ggacggctgg gacgaactgc acgcccggat gcttcacacc gcccgcgcca gaggattcgg
     2581 ggtcttcacc ggagatacgg gaacggggaa gacgacgtcg ctcagacgct tcgcgggcga
     2641 actggacccc aaccgatacc gcgtgctgta cgtctgcgat tccttcctga ctccccgcaa
     2701 cttctactgg gaggtgctgc accagctcgg ttgcactcca cgcttctatc gcggagacgc
     2761 gaagcggcag ttccagaagg ctctgagaga agtgaacgag gagaggaaga acccggtggt
     2821 cgtggtggac gaggcgcatc tgctctcccg cgaaatgctc gaggagatcc gttttctcct
     2881 caacaccgga atggactccg agagctcgct ggcgctcttg ctggtgggac aaagcgaact
     2941 gaaggacgcg ctgaaactcc aggtgaacca ggccatagcg ggaagggtgg atatccgctt
     3001 ccatctggga gccatggaca aagggcagac cgcttcgtac gtcaaacggc acatggactc
     3061 ggtgaaagcc cccagagaca tttttacgtc gacggcgctt ggaatgatcc acgagtacgc
     3121 cggaggaatt ccgcggaagg tgaataaagt cgcgtcgtcg tgtctgatga ctgcggcggt
     3181 caggaaagag actcttgtgg acgaccatct tgtgcaggag gttttggaga gcgaattcga
     3241 aatttgagcc ggaggggcgg tatccttccg ccccttacgg acacgaatgg aaagctgtct
     3301 ccgcggtcat cgaaaaagag tcgaccccgg acaaggagat caagccgcta ca
//
LOCUS       DAOSWW010000191         3325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20647_length_3325_cov_3.827829, whole genome shotgun sequence.
ACCESSION   DAOSWW010000191 DAOSWW010000000
VERSION     DAOSWW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20647_length_3325_cov_3.827829"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..591)
                     /locus_tag="PK442_05055"
     CDS             complement(103..591)
                     /locus_tag="PK442_05055"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOO87049.1"
                     /translation="MAQKVYKGSGADFITAVCLVLFGAAFSIAALRMRVFNNSLLVSP
                     GLFPLILGAVFILLGLLLLRSAAKRGGWAQASHVLGKEHLHTFATSPRVRKAVVLLLF
                     IVAYVAAVAHFPFLWATVGYLLVTFIYLKAMKLHWILLVSFLAAYAITFAFRDLFQIP
                     MP"
     gene            complement(593..1648)
                     /locus_tag="PK442_05060"
     CDS             complement(593..1648)
                     /locus_tag="PK442_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOO87050.1"
                     /translation="MKKFVSMVAIALVIVFVIAGGAVAAEKVWEKGTPTVIVGFGAGG
                     GTDTSVRPVVALMSKYLNETINVANMPGAASAVAAEHVLGLPADGYTMFATGSGCFGG
                     FKVKETSNSGWEEWSSWHPFQGPAAILVAANGELKTFQDVIDALKTGKINMGASGFGN
                     GPHVIMQAIAEVAGIKEINYVTFDGDRSIATAVLAGEIEAGIVTFSSAIDMAQAGTIK
                     AVVVMTREPMKITDTITIDPIVKVLPGSDKIPDLGETWPICIRRDAPQEIKDKITEAF
                     LWAIKQPEIEEYTKKKGLNIVGLTGEDADRFISLQISGYAWVLHNSGMTEKSPEEFGI
                     PKLADWNWETEKKKLTK"
     gene            complement(1783..2682)
                     /gene="garR"
                     /locus_tag="PK442_05065"
     CDS             complement(1783..2682)
                     /gene="garR"
                     /locus_tag="PK442_05065"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HOO87051.1"
                     /translation="MEAILMSTIGFIGLGIMGKPMAKNLLAGGLSLVVYDVVSAAVDE
                     VVAAGAAKGTSPKDVAEKSDIVVTMLPNSPHVREVVCGKNGILEGTKKGQIVVDMSSI
                     APLASRELGAMLAEKGAEMLDAPVSGGQEKAEKGTLAIMVGGSEASYEKVKDILALMG
                     KSTLVGELGAGQTTKLVNQTIVAINIAAVAEGMVFAKKAGVDPEKVFQAIRGGLAGSQ
                     CMEDKAPRMFEGRYNPGFRINLHIKDLTNVLETSRSLHMSMPLAAQLMEMFQNLAGEG
                     HETLDHGALGLFYEKLNSLSLKK"
     gene            complement(2696..>3325)
                     /locus_tag="PK442_05070"
     CDS             complement(2696..>3325)
                     /locus_tag="PK442_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOO87052.1"
                     /translation="AFAIDRGGLVGGDGATHQSFEDLATMRVLPHMTVLCPADGIEVK
                     WAVRAAAAVDGPVYIRLNRNDLPVLFDEKTSFEIGKPLVVKEGNDVALVCTGTMTSIA
                     LDAAALLEQQGISARVVHLGTIKPLCGKSLREALGNVRGIVSCEEHTIYGGLGSAVAE
                     ALCEAPLPMKMVGIQDRYGQSAVCYEELLECYGLTKEAVAKAAEALLKK"
BASE COUNT          587 a         1033 c         1011 g          694 t
ORIGIN      
        1 caggccgaga atggtcgggt tgagcgctcc cagcagggag cctcccacct gtacagcttc
       61 gtcagcgaga agatgcagta tctccatgta tccgtccctc ctttacggca tcgggatctg
      121 gaagagatcc cgaaaggcga atgtgatggc gtaggccgcc agaaaggaga ccagcagtat
      181 ccaatgcagc ttcatcgcct tgaggtagat gaaggtgacc aggaggtagc cgacggtagc
      241 ccagagaaag ggaaagtgcg ccactgcggc cacgtacgcg acgatgaaga gaagaaggac
      301 gaccgctttg cggactctcg gcgacgtcgc gaacgtatga aggtgttcct ttcccagaac
      361 gtgcgaggcc tgtgcccacc ctcctcgttt cgctgcggag cggagaagga gaagcccgag
      421 gaggataaag accgctccga gtatcagagg gaaaagcccc ggcgaaacga ggagggagtt
      481 gttgaacacg cgcatcctca gggcggcgat ggagaacgcc gccccgaaga gcaccaggca
      541 aacggccgta atgaaatcgg ccccggatcc cttgtaaact ttttgcgcca tgctacttcg
      601 tcagcttctt cttttcggtc tcccagttcc agtcggccag cttgggaatg ccgaattcct
      661 cgggcgattt ctcggtcatt cccgaattat gaagcaccca ggcgtatccg gagatctgga
      721 gcgaaatgaa gcggtccgcg tcttcgccgg tgaggccgac gatattgagg cctttcttct
      781 tcgtgtactc ctcgatttcg ggctgtttga tggcccagag gaaggcttcg gtaattttat
      841 ccttgatctc ctgaggggcg tcgcggcgga tgcagatggg ccacgtctcg ccgaggtcgg
      901 gaatcttgtc gcttccgggg agcaccttga cgatcgggtc gatggtgatc gtatcggtga
      961 tcttcatcgg ctctctcgtc atcacgacga ccgccttgat ggttcctgcc tgcgccatgt
     1021 cgatggcgga tgagaaggtc acgatgccgg cctcgatctc gccggcgagg accgccgtgg
     1081 cgatgctccg gtcgccgtcg aaggtgacgt agttaatctc tttgataccc gccacttcgg
     1141 cgatcgcctg catgatcacg tgagggccgt tgccgaagcc ggaagcgccc atgttgatct
     1201 tgccggtctt cagggcgtcg atgacgtcct ggaaggtctt cagctcgccg ttcgccgcga
     1261 cgaggatggc tgcggggccc tggaagggat gccaggagct ccactcctcc caccctgagt
     1321 tggaggtctc tttcaccttg aagccgccga agcagcccga accggtggcg aacatcgtgt
     1381 atccgtcggc gggaaggccg aggacgtgct ccgcggcaac cgcgctcgcc gcgccgggca
     1441 tgttggcgac gttgatcgtt tcgttcagat acttcgacat cagggcgacc accggacgga
     1501 cggatgtgtc cgtgccgccg cctgcgccga agccgacgat gacggtgggc gttccctttt
     1561 cccatacttt ctccgcggcg actgcgccgc ccgcgatgac gaagacgatg acaagtgcga
     1621 ttgcaaccat tgaaacgaac ttcttcacaa cgaccactcc tttttgatat gaaaggttgt
     1681 gttgctcgtg ttccggaagc ttttcgaacg gtgcgcgact ggcttgtcac aatttttccc
     1741 ggcgcatccc ccctagcata gtctgtccgc gtgcgcggac ggttattttt tcagcgacag
     1801 gctgttcagc ttctcgtaga agagaccgag ggcgccgtgg tccagcgtct cgtgcccttc
     1861 tccggcgagg ttctggaaca tctccatgag ctgggccgca agcggcatcg acatgtgcag
     1921 cgaacggctg gtttcgagga cgttggtgag gtccttgata tgcaggttga tgcggaagcc
     1981 cggattgtag cgcccttcga acatgcgggg cgccttgtcc tccatgcact ggctgccggc
     2041 aaggccgccg cggattgcct ggaagacctt ttccgggtcc actccggcct tcttcgcgaa
     2101 gaccatcccc tcggccaccg ccgcgatgtt gatcgccacg atggtctggt tgacgagttt
     2161 cgtcgtctgt cccgcgccga gttcgccgac gagcgtgctc ttgcccataa gcgcgaggat
     2221 gtccttcacc ttctcgtaag aggcttcgga gccccccacc atgatggcca gggttccctt
     2281 ctcggccttt tcctgccctc cgctgaccgg ggcgtcgagc atctccgctc ccttttcggc
     2341 gagcatggct cccagttcgc ggctcgccag cggcgcgatg gagctcatgt cgacgacgat
     2401 ctgccctttc ttcgttcctt ccaggatgcc gttcttgccg cagacgacct cgcggacgtg
     2461 cggggagttc ggcagcatgg taacgacgat gtccgacttt tccgcgacgt ctttcggtga
     2521 cgtccccttc gcggcgcccg ccgcgacgac ttcatccacc gcggcggaaa ccacgtcgta
     2581 aacgacgagt gacagccctc cggccagaag attttttgcc atgggcttcc ccatgatgcc
     2641 gagcccgatg aatccgattg tgctcattag aatcgcctcc atatatgaat atcttttact
     2701 ttttgagcag tgcttccgcc gccttcgcaa ctgcctcttt ggtaagaccg tagcactcca
     2761 gcagctcctc gtagcagacg gccgactggc cgtaccggtc ctggatgccc accatcttca
     2821 tcggcagggg cgcctcgcaa agggcctccg cgaccgccga ccccaatccg ccgtagattg
     2881 tgtgctcctc gcagctgacg atcccccgga cgtttccgag agcttcccgg agcgacttcc
     2941 cgcagagggg cttgatcgtc ccgagatgga cgacgcgggc ggagatgccc tgctgttcga
     3001 ggagcgccgc cgcgtcgagc gctatcgagg tcatcgtccc cgtgcagacg agggcgacat
     3061 cgttcccctc tttgaccaca agcggcttgc cgatctcgaa ggacgtcttc tcgtcgaaga
     3121 gcaccggcag gtcgttgcgg ttcagacgga tatagaccgg cccgtcgacg gccgccgcgg
     3181 cgcgcacggc ccatttcacc tcgatcccgt ccgccgggca gagaacggtc atgtgcggaa
     3241 ggacgcgcat cgtggcgagg tcctcgaacg actggtgcgt cgcgccgtcg ccgccgacca
     3301 ggccgccgcg gtcgatcgcg aacgc
//
LOCUS       DAOSWW010000192         3321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20680_length_3321_cov_4.491733, whole genome shotgun sequence.
ACCESSION   DAOSWW010000192 DAOSWW010000000
VERSION     DAOSWW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20680_length_3321_cov_4.491733"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK442_05075"
     CDS             complement(<1..215)
                     /locus_tag="PK442_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOO87053.1"
                     /translation="MFPFFFDPTMILLIPALLLAMWAQMRVKSVFANYSRVGSRRGVT
                     ASAVARALLDRFGLRDVPVQRVGGNLT"
     gene            complement(329..1462)
                     /locus_tag="PK442_05080"
     CDS             complement(329..1462)
                     /locus_tag="PK442_05080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO87054.1"
                     /translation="MATKIPTLDLVRNYSRIKDEILEAVHGVLETQHFILGPDVSLFE
                     KECEQYLGGVSAVACASGTDALILALMSLDIKEGDEVITTPYSFFATASCITRLGAVP
                     VFVDVDPATFNVDMNKVLEATTPRTKVFLPVHIFGQMAPLEDGMKELTSRGITVVEDA
                     AQAFGAWRSVEGDILRAGAVGRIGCFSFFPTKNLGGYGDGGMVVSTDTQLADRLRKLR
                     VHGAGTTYFHDEVGLNSRLDSLQAAILRVKLRHLEEWNEERRIVAGRYYALFAEKGLL
                     ETVQPPVELEGNHHIYHQYVVRVPQRDDLQRFLEEEGVVSRVYYPLSLHLQRCFSFLG
                     YKQGDFPVSERLSKETLALPVFPELSLEEQRRIVAAIGKFYGK"
     gene            complement(1628..2287)
                     /locus_tag="PK442_05085"
     CDS             complement(1628..2287)
                     /locus_tag="PK442_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO87055.1"
                     /translation="MREPRLYTTSADYAYQELRHRIITKQLKPGQRLPEVNIAVQMGV
                     SRTPVREALRRLASEGLVLIIPNSGARLAAPTIREIEDTFLVREQLECLAIALAAERI
                     GERHLRRIEEALVEEARAIEDKDLETYLEVNEAFHKTVADASGNRVLAEYVENILART
                     NAYVVFYDPFYHVETSPSPDDHRDILASLRAHDKEKSVALMKAHLEEAVAGLGKIVVE
                     E"
     gene            complement(2709..>3321)
                     /locus_tag="PK442_05090"
     CDS             complement(2709..>3321)
                     /locus_tag="PK442_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOO87056.1"
                     /translation="ILSVRRKKGFFILEDDSYGELRYGEKSIPALKAMGEGEGVVYVG
                     SFSQALFPGMRLGYTLLPPFLKEEYLRISSLRQGYVSSLVQLVVARFLEDGELAAAVE
                     RARTILSVRMETLYSLLKSHFSTVPLRKPEGGVFLWFPTGKTDGSEAALLAERHGVRV
                     APGSRFSLRKRKIAAVRFSITGVPDELLNEAVARLKKAWGKIL"
BASE COUNT          834 a          885 c          770 g          832 t
ORIGIN      
        1 tccgtcaagt tgccgcctac tctttgaaca gggacatctc tcaaaccaaa tcgatcaagc
       61 aaggccctcg cgacagccga tgcagtaact ccccgacggg agccgacgcg gctgtagttt
      121 gcaaagacgc tcttcactct catctgcgcc cacatagcca gtaaaagcgc tggaatcagc
      181 aatatcatcg tcggatcaaa gaaaaaagga aacactgttt ctcacctccg ctatatgctc
      241 tcattctacg aggaaagggt acactgcgtt cccctttcct ccatcggtat tttacacggt
      301 tgtatgagaa agagcaatat tggtcagact attttccgta aaatttcccg attgctgcaa
      361 caatacgccg ctgctcctct agagagagtt cagggaagac cgggagagca agagtttcct
      421 tgctaagccg ttctgaaacc gggaaatcgc cttgtttata accaaggaaa gagaaacacc
      481 tttgcagatg aaggctcagc ggataataga ccctgcttac tactccttct tcctcaagga
      541 aacgctgaag gtcgtctctt tgcggaaccc gtacgacata ctgatgataa atatgatgat
      601 tgccttcgag ttccactggc ggttgcaccg tttccagaag acctttttcc gcaaaaagag
      661 cataatatct tccggcgacg atacgccgct cttcgttcca ctcctcaagg tggcggagtt
      721 ttacacgcaa aatggccgct tgaagcgagt cgagacggct gttcaagcca acctcatcat
      781 ggaaataggt agttccggcg ccgtgaaccc tcagcttgcg aagacgatct gccagttgcg
      841 tatccgtcga gacaaccatc ccgccgtcgc cgtatcctcc caggtttttg gtagggaaga
      901 atgaaaaaca accgatgcgc ccgactgctc cggctcgaag aatgtcccct tcaacagaac
      961 gccaggcccc aaaagcttgc gctgcgtctt cgacgactgt aataccgcgg gaagtaagct
     1021 ctttcatgcc atcttcgagg ggcgccatct ggccgaaaat atgcacggga agaaaaactt
     1081 ttgttcgcgg cgtcgtcgcc tcaagaactt tgttcatatc gacattgaat gtcgcaggat
     1141 cgacgtcgac aaaaacgggg acagccccaa gacgtgtaat acagcttgcc gttgcaaaga
     1201 agctgtacgg cgttgtgatt acctcatccc cctcttttat atcgagcgac atgagcgcaa
     1261 gaatcagcgc atctgttcca gacgcgcacg caactgctga cacgcctcca aggtactgct
     1321 cgcactcttt ttcaaagaga gagacatccg gtccgagaat gaaatgctgt gtctcaagca
     1381 ctccgtgcac cgcttcaaga atctcgtctt ttattcttga ataattacga acaagatcta
     1441 gcgtgggtat tttcgtcgcc atgaaaaaag tacctcccgt aaaagtgagc gaaacagcgt
     1501 ttcgctctca taagaactcc attgaatatc ttcacttcat tcggcgaatt ttggcattga
     1561 gcaaaaaaac aagcgaacgc tccgtccggg gcgatcgcct aaacaacttg agaatgaaga
     1621 aagtagtcta ctcttcgacg acaatcttcc caaggcccgc aacagcctct tccaagtggg
     1681 ctttcatcaa agcgacgctc ttctctttgt cgtgagctct gagacttgcc aggatgtctc
     1741 tgtggtcgtc agggctcggc gaagtctcca catgataaaa ggggtcgtaa aacacgacgt
     1801 aggcattcgt ccgtgcgagg atattctcaa cgtactccgc gaggaccctg ttcccgcttg
     1861 catccgccac tgttttatgg aacgcttcgt tgacctccag gtaggtttca agatctttat
     1921 cttcgatcgc tctggcctct tcgaccaggg cctcctctat gcggcgaaga tgacgttctc
     1981 caatacgttc ggcggcaagc gcaatggcaa gacattccaa ctgctcgcgc acaagaaatg
     2041 tatcttctat ctcccggata gtaggagccg caagccgggc gccgctgttc ggtatgataa
     2101 ggacaagccc ttcgcttgca agacggcgaa gagcttctct taccggggtt ctgctaaccc
     2161 ccatctggac tgctatattt acttcgggca gacgctggcc cggttttagt tgttttgtga
     2221 tgatacgatg gcgaagttcc tggtatgcat aatcagcaga cgtggtgtac aaacgcggct
     2281 ctctcatgga aggacctccc tgtaattttc taaattgatc gagagtattt caatcattgt
     2341 ataacagtct gtattatata tcttgaacca ctttatcaca ttcaatgcaa aaccacaaga
     2401 tgcggctgct ttttttttga tgctataatt gcctttttga aaagaaaaga ggggacgaac
     2461 gtgcgatttt tggtgttggt gctttccatg tccgctgtag tgtttttcgg atacgcggtg
     2521 gctggtcttg ccggctttct tcttggaaga ggaagatctg actatattat attcggcctg
     2581 ctcctcggtt tcgcatctac cgcagcgtcg cttctacttt ggaggcattg gctctcactc
     2641 cttgagaaag aggcgctgca caccgcagat aatacgggag aaaaactctc ctgacggcat
     2701 gcccagtttc agagaatttt tccccatgct tttttcagac gtgcaacagc ttcattcaac
     2761 aattcatcag gaacgccggt tatagaaaaa cggacagcgg ctattttcct ttttcgtaga
     2821 gaaaaacggc tcccgggagc gacgcgtacg ccatgtcttt cggcaagcaa tgcagcctca
     2881 gatccatctg tcttccctgt agggaaccag agaaacactc cgccctcagg tttcctgaga
     2941 ggcactgtac tgaaatgact ctttaaaaga ctgtaaagcg tttccatgcg gaccgaaaga
     3001 attgttctgg ctctttcaac cgcggccgcc agctctccat cctcaagaaa acgtgcgact
     3061 accagctgta cgagagagga aacgtatccc tgtctcaaag aacttatccg caaatattcc
     3121 tccttgagaa aaggaggaag cagcgtgtat cccagccgca ttcccgggaa gagcgcctgg
     3181 ctgaaggagc ctacataaac gacgccttcc ccttccccca tcgcctttaa ggccgggatg
     3241 gatttttctc catatcggag ttctccgtaa ctgtcgtcct caagaatgaa aaaacccttt
     3301 tttctcctga cagagagtat c
//
LOCUS       DAOSWW010000193         3316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20719_length_3316_cov_4.462435, whole genome shotgun sequence.
ACCESSION   DAOSWW010000193 DAOSWW010000000
VERSION     DAOSWW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20719_length_3316_cov_4.462435"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PK442_05095"
     CDS             complement(<1..899)
                     /locus_tag="PK442_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO87057.1"
                     /translation="MEKENTQYLKMIGMPVGKLILSLSVPTIVSMLVTAIYNIGDTYF
                     VSQLGTSASGAVGIVFSLMAIIQAVGFTVGMGSGAQISRLLGAKKVDEANTVAASGLF
                     AAIGLGSLVALFGLLFLDDLMRVLGATETILPYARDYASYILYAAPVMASSFLLNNIL
                     RAEGKAKFAMVGLTTGGLLNLALDPLFIFGFGWGIAGAAIATAISQCVSFGILFSWFL
                     RGKTIVRLSAASISRSPLEYLRIFKNGFPSFCRQALASIATIALNVNAAVYGDAAVAA
                     MSIVGRLFMFVFVVNLGIGQGYMP"
     gene            1144..1389
                     /locus_tag="PK442_05100"
     CDS             1144..1389
                     /locus_tag="PK442_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87058.1"
                     /translation="MPTLQVRDLPEELYQKMLYLAEKEHRSLTQTTIVLLKEGLERRL
                     ADTERRRDLLRNFSGIGPDAAGLPDAEILVREDRDRR"
     gene            1386..1784
                     /locus_tag="PK442_05105"
     CDS             1386..1784
                     /locus_tag="PK442_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOO87059.1"
                     /translation="MIVVVDASAAVEIVLGKPSANRFREELLDAALTLAPDTYASEIT
                     NVFWKYRAYSGLPEQACLEGIEFCLGIVDEYIPSVQLWREAFFEASKAKHPVYDMLYL
                     IVARRHNARLLTKDGKLAELASQSGIFVVS"
     gene            complement(1814..2458)
                     /locus_tag="PK442_05110"
     CDS             complement(1814..2458)
                     /locus_tag="PK442_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HOO87060.1"
                     /translation="MEEIRMETWDRREHFEFFLRNDLPFYNVNFNADITGLREYAKGA
                     SVSLSSALIYLVTKSLNMVPNFLYRVLGGKVVRYERLHPQFTCLRGDEELFRLVTVEF
                     CDDLAEFDRSVKRAIEESDAYFNVGMLAHRSDFVFISSLPWIPFTGIDHTLSLNKEDA
                     IPRVSWGRFFEQDRRTLLPCNIRVNHIFIDGLHVGRFYETFQEETRRLADSSAR"
     gene            complement(2533..2940)
                     /locus_tag="PK442_05115"
     CDS             complement(2533..2940)
                     /locus_tag="PK442_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOO87061.1"
                     /translation="MILVDTSVLVGYLKGSAGAVFDRFDEMIAGGVPFGINGYIYQEV
                     LQGARDEREYALLAEYLSSLPFYSLLHGRASFESAARIFFLCRRSGVTVRSTVDLLIA
                     QIAIENELYLFHNDRDFSNMAKIAPELKLIDYF"
     gene            complement(2943..3140)
                     /locus_tag="PK442_05120"
     CDS             complement(2943..3140)
                     /locus_tag="PK442_05120"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOO87062.1"
                     /translation="MRTNIVIDDELMEEAFTVSGGVSTKKELVELALREFVDTRKRRD
                     LRELRGRVAFADEYDYKSMRQ"
BASE COUNT          746 a         1036 c          905 g          629 t
ORIGIN      
        1 ggcatgtacc cctgcccgat ccccagattg accacgaaga cgaacatgaa gagcctgccg
       61 acgatggaca tcgcggccac ggcggcgtct ccgtacacgg cggcgttcac gttgagcgcg
      121 atggtcgcga tgctggcgag cgcctgcctg cagaaggagg ggaagccgtt cttgaatata
      181 cgaaggtact ccaacggact gcgcgagata ctcgccgcgc tcaaacgtac gatggtcttc
      241 ccccgaagga accaggagaa caggatgccg aagctgacgc actggctgat cgccgtcgct
      301 atagcggccc cggcgatacc ccacccgaag ccgaagatga acagggggtc gagcgccagg
      361 ttgagcagac cgccggtcgt cagcccgacc atggcgaact tcgccttccc ttccgcccgg
      421 aggatgttgt tcagcagaaa ggacgacgcc ataacgggcg cggcgtacag aatatagctc
      481 gcgtagtccc gcgcgtacgg cagaatcgtc tccgtcgcgc ccagcacgcg catcagatcg
      541 tcgagaaaga gcaaaccgaa gagcgcaacc agcgacccga gcccgatcgc cgcaaagaga
      601 ccgcttgccg ccaccgtatt cgcttcgtct accttcttgg cgcccagcag ccgcgagatc
      661 tgcgcgccgg aacccatgcc cacggtgaag cccaccgcct ggatgatcgc catcagcgaa
      721 aagacgatgc ccaccgcgcc cgaagcgctt gtcccgagct gcgagacaaa gtaggtatcc
      781 cctatgttgt agatcgccgt caccagcatg ctgacaatcg tcggaaccga cagcgacaga
      841 atcagcttgc cgaccggcat ccctatcatc ttgagatact gcgtgttttc tttttccatt
      901 gatcgaactc ttctttcttt tgcgctgtgc gaaaaatctg cggggatgag ctcacccacc
      961 gcctattgta tcgactcacg gtgacttttt ctgtattcat tccctcgggg tacacttgaa
     1021 atatcgcttg aagacgtgcg cttagaatcc ttcggaaaca cgccatgttg caatgcgcgc
     1081 tccgaggagc ccttgattcg catgcaaaaa acatctagaa tgcatgaaag ggaggtggga
     1141 atcgtgccga cgcttcaggt tcgggattta cccgaagagc tgtatcaaaa aatgctctac
     1201 cttgcggaga aggagcaccg aagccttacg caaacgacga tcgttctgct gaaggagggg
     1261 ctcgaacggc gccttgccga cacggagcgc cgccgcgatc tgctccggaa cttttccgga
     1321 atcggccccg acgccgcagg tctgccggat gcggagattc tcgtccgaga ggacagggac
     1381 cgtcgatgat cgtcgtcgtc gacgcaagcg ccgcagtgga gatcgtcctc ggaaaacctt
     1441 cggcgaaccg gttccgcgag gaacttctcg acgccgcgct gacgctcgcg ccggatacct
     1501 acgcctccga aataacgaac gtcttctgga agtaccgcgc atactccggg cttcccgagc
     1561 aggcgtgtct ggagggaatc gaattctgcc tcggcatcgt cgacgagtac atcccctcgg
     1621 ttcagctctg gcgggaggcg tttttcgagg catcgaaggc gaagcacccc gtgtacgaca
     1681 tgctctatct catcgttgcg cggcgacata acgcccgcct gctcacgaag gacggaaagc
     1741 tcgccgaact cgcctctcaa agcggaatct tcgtcgtttc gtaaacagcc gactcccgca
     1801 agggaaaccg gcctcagcgg gccgacgaat ccgccagtct gcgcgtttct tcctgaaacg
     1861 tctcgtagaa acgccccaca tgaagcccgt cgatgaagat atgattcacc cggatattgc
     1921 acggcagcag cgtccgcctg tcctgctcga agaaacgccc ccacgaaacg cggggaatcg
     1981 cgtcctcctt gttcaggctc agcgtgtggt cgatccccgt aaacggtatc cacggcaggg
     2041 aggagatgaa gacgaagtcg ctcctgtgcg ccagcatccc cacgttgaaa taggcgtcgc
     2101 tctcctcgat cgcgcgcttg acgctccggt cgaactccgc aaggtcgtcg caaaactcca
     2161 cagtgaccag ccggaagagt tcctcgtcgc cccgaagaca ggtgaactga gggtgcagcc
     2221 gctcgtagcg caccaccttg ccgccgagca ctctgtagag gaagttcggc accatgttca
     2281 gcgacttcgt caccaggtag atcaacgcgg agctcagcga gacggaagcc cccttcgcat
     2341 actccctcag gccggtgata tccgcgttga agttcacgtt gtaaaagggg agatcgttcc
     2401 tcaggaagaa ctcgaaatgc tctcgtctgt cccacgtctc catacgaatc tcttccatgc
     2461 aaacctcctc cggacgcccc cgacacgtaa gaacgacacg tcaaaaaact tcgacggaat
     2521 tccgtcgaag cgctaaaaat agtctatcag cttgagttcg ggggcaatct tcgccatgtt
     2581 tgaaaaatct ctgtcgttgt gaaagagata cagttcgttt tcgatggcga tctgagcgat
     2641 cagcagatcg acggtgctgc gcacggtgac gccggaacgc ctgcacagaa agaagatacg
     2701 ggctgctgac tcgaacgacg cccgcccgtg aagcagcgaa tagaaaggca aagaagacag
     2761 atattcggca aggagagcat actctctttc atccctcgcc ccctgaagca cctcctgata
     2821 gatgtacccg ttgattccaa acggaacgcc gccggcgatc atttcgtcga acctgtcgaa
     2881 aacggcgccc gcgctccctt tgaggtaccc cacaaggaca gacgtatcca ccaggatcat
     2941 actcactgcc tcatggactt gtagtcgtac tcgtccgcaa aggcgacccg cccccgaagc
     3001 tcgcggagat cccttctctt tctcgtgtcc acgaactccc tcagggcgag ttccacaagc
     3061 tcctttttcg tcgaaacgcc gccggaaaca gtgaaggcct cttccatcag ctcgtcgtcg
     3121 atgacgatat tcgtgcgcat acacatcacc tcatacacat tataatacac acgaacgccc
     3181 cgagcaatca gggcaggagt gtcccgtttg ttcagggcgt gtcggggagc cgggaagagg
     3241 tgtatcgcgc tccaccccca ccgcgcgctt ctccgggttc agatcgtcgg cgcgatgacg
     3301 tagtcgacac gctcgt
//
LOCUS       DAOSWW010000194         3311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20770_length_3311_cov_3.585995, whole genome shotgun sequence.
ACCESSION   DAOSWW010000194 DAOSWW010000000
VERSION     DAOSWW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20770_length_3311_cov_3.585995"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3311)
                     /locus_tag="PK442_05125"
     CDS             complement(<1..>3311)
                     /locus_tag="PK442_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOO87063.1"
                     /translation="HADGPATVFLQVKEWNDNVADPSSYILGTFFEPCDQLDAQGRND
                     SPDTASPLELNEWTRGNLLPNGDNDWFRIDVDHPGILRLEGTTPPGIWVRLALHGGNA
                     EQLADVGYSRPSSEFTWENPVLPGAYFIRLREWNNNASNPGIYSLRAVLQRSEPEESV
                     PLAEDAVRTLTPGEARPFFIDHGGDRDVFLFDIAQQGPFWLKIWKPGNIWIRARLYDD
                     RTGAELKKIDLSGASYNGKFEAEGPTRYRLELGEWNNNAYSVQPGFIQVRPDDAQITA
                     DEIKAVPDAVTPTLVTFTRSAWAHMQIPRARTIEVDATGDGKTDFSFRPGDTATWRYP
                     AEGLYKAAALMQGEGGATTRLPFWVDVSGPRERTGIHLTVDAPGEGQRVEDEAPARAR
                     AMSYSGSPIARIEASVDGTQLPPAYTPPYTFEVPWKSLPRAAKEHLFTFKAVDGRGES
                     ATVERRVSISEYFGLRPADGAVLSGNQVRVSWSSGEFSPTKVRYRAKGTEEWTEIVGE
                     SGREHAVFLEDLESGTAYEFQPLGGSEEGPVRTLTRVKGLAFGKSRYGASIERTYDQR
                     FPISVRNHAEEAMKVVLTSGKLEEEGLFIGFVGEGSEGEPFDLAPGEEREFLLVLNAQ
                     NAVKPKHVFPVKISSANGFADEAEVEVEVRLPVVKLEWEDLGEAPKGPGRRLRLVNRG
                     DSLTDLSVFTDTGELKVSPAINHGFLPSGQSVELTVTPRLYEGFREARGAVTAKSIAT
                     ESSFPVHLALPEGKEIFGVNLMPKMPEETADEDERVLLEARALSAAFLTPDYIDASMW
                     GNGIDSNGDGRIDRWSFIDQEERILWVGEDASGDGEIDFVRADVGFDGRFDYSALKGA
                     KGWEETNLLDAWLEMTFTLPWNRGTYEPHDLDIVMNGQVIGTVKNAIPEGNYRFPVPA
                     SAIRFGASGEPEGNGVEIVSRHLRGGHYVVNSDFRLVTRLNAARAWTVAESREEAEAA
                     ARSMEGVIADRAKISISSQGISLPEGGSLKAGAETTLTATLRNLGAMPGYNVGVVLSI
                     ADPNGTERELRRSYIPFVPVTGEIPVRFTFSLPPGKHRLKLSLNPDNRPENSAYIWAE
                     VGGDDQRPTLDLLEP"
BASE COUNT          517 a         1133 c         1043 g          618 t
ORIGIN      
        1 aggctccagc aggtcgagcg tcggccgttg gtcgtcgccg cccacctcgg cccagatgta
       61 cgccgagttc tcagggcggt tatccgggtt cagcgagagc ttcaggcggt gtttgcccgg
      121 aggtagtgag aaggtaaagc gaaccggtat ttctcccgtc accggaacga aggggatgta
      181 gcttctccgc agctctcgct ccgtcccgtt cggatcggcg atgctcagaa cgacgccgac
      241 gttgtacccc ggcatcgcgc caaggttccg cagcgtcgcg gtcagcgtgg tttcggcccc
      301 ggctttgagc gagccgccct cgggaagcga gattccctgc gaggagatgc tgatcttcgc
      361 cctgtcggcg attacgccct ccatggagcg ggccgccgcc tccgcttcct cgcgcgactc
      421 cgcgacggtc catgcgcgcg ccgcgttgag gcgcgtcacc agccggaagt cgctgttcac
      481 gacgtagtgg ccgcccctca ggtggcgcga gacgatctcg acgccgttcc cttcaggctc
      541 gccgctcgct ccgaaacgga tcgccgacgc gggaaccggg aagcggtagt tcccctcggg
      601 aatcgcgttc ttgactgtgc cgatcacctg accgttcatc acgatgtcga ggtcgtgcgg
      661 ctcgtaggtt ccgcggttcc atggaagggt gaaggtcatt tcgagccaag cgtcaagcag
      721 gttggtctcc tcccacccct tcgcgccctt gagcgccgag tagtcgaagc ggccgtcaaa
      781 gccgacgtcg gcgcggacga agtcgatctc tccgtccccg ctcgcgtcct ctccgaccca
      841 gaggatgcgc tcctcctggt cgataaagct ccatcggtcg atcctcccgt cgccgttgga
      901 atcaatcccg ttcccccaca tggaggcgtc gatgtagtcg ggcgtcagga aggccgcgga
      961 gagcgccctg gcctcaagga gcacacgctc atcttcatcc gccgtctcct ccggcatctt
     1021 cggcatcagg ttgacgccga agatctcctt cccttcggga agcgccaggt ggacagggaa
     1081 cgacgactcc gtggcgatgg acttcgccgt caccgcgccc cgcgcctcgc ggaacccctc
     1141 gtagagcctc ggcgtcacag tgagctccac agactgcccc gaagggagaa atccgtggtt
     1201 gatcgccgga gagaccttca gctcgcctgt gtccgtgaag accgaaagat cggtaaggct
     1261 gtcccccctg ttcaccaggc gcagacggcg tcccggcccc ttcggcgctt cgccgagatc
     1321 ctcccactcc agcttcacga cggggaggcg cacctccacc tccacctccg cctcgtcggc
     1381 gaagccgttc gcggagctga tcttgacggg gaagacgtgc tttggcttca cggcattctg
     1441 cgcgttgagg acgagcagga actcccgctc ctcgcccggc gccagatcga acggctcccc
     1501 ctccgacccc tcgccgacga agccgatgaa gagcccctcc tcctctagct tgccggaggt
     1561 gagcacaact ttcatcgcct cctcggcgtg gtttctgacc gaaatgggga accgctggtc
     1621 gtaggtgcgc tcgatggacg cgccgtagcg gcttttgccg aaggcgagcc ccttgacccg
     1681 cgtgagggtc cgcaccggcc cctcctcgct ccctccgagc ggctggaact cgtacgccgt
     1741 tccggattcc agatcctcaa ggaatacggc gtgttcgcgg ccgctctcgc ccacgatctc
     1801 cgtccactct tccgtcccct tcgccctgta gcggaccttc gtcggcgaga actccccgct
     1861 cgaccagctc acgcggacct ggttgccgga aagcacggcc ccgtccgccg gacgcaggcc
     1921 gaagtactcg gagatcgaga cgcggcgctc gacggttgcg gattcgcccc gcccgtcgac
     1981 ggctttgaac gtgaagagat gctccttcgc cgcgcgggga aggctcttcc acggcacctc
     2041 gaacgtatag gggggcgtgt aggcgggcgg gagctgcgtt ccgtcgaccg aggcctcgat
     2101 gcgcgcaatg ggcgagccgc tgtagctcat ggccctggcg cgcgccggag cctcatcctc
     2161 cacgcgctgc ccctctcccg gagcgtcgac cgtcaggtga atccccgtcc gctcccgtgg
     2221 gccggagacg tccacccaga agggaagccg cgtcgtcgcg ccgccctcgc cttgcatcag
     2281 cgcggcggcc ttgtacagcc cctccgcggg gtagcgccac gtggccgtgt cgcccggacg
     2341 gaatgaaaag tcggttttcc cgtcgcccgt cgcgtcgact tctattgtgc gtgcgcgggg
     2401 gatctgcatg tgcgcccacg cgctccgggt gaaggtcacg agcgtcggcg tcacggcgtc
     2461 gggaaccgcc ttgatctcgt cggcagttat ctgcgcgtcg tccggacgca cctgaatgaa
     2521 gccgggctgg acggagtacg cgttgttgtt ccactccccc agttcaagcc ggtagcgcgt
     2581 cggtccctcc gcttcgaact ttccgttgta ggaagcgccg gagagatcta tcttctttag
     2641 ctccgctccc gtcctgtcgt cgtagaggcg cgcgcggatc cagatgttcc caggcttcca
     2701 tatcttcagc cagaacggcc cctgctgtgc gatgtcgaag agaaagacgt ccctgtcgcc
     2761 tccgtgatcg atgaaaaacg ggcgcgcttc tcccggcgtg agcgtgcgga ccgcgtcctc
     2821 ggccagcggt acgctctctt ccggctcgga ccgctggagc accgcacgga gagagtagat
     2881 gccggggttg gaggcgttgt tgttccactc ccggaggcga atgaaataag ctccggggag
     2941 aacggggttc tcccatgtga attcagagga ggggcgtgaa tatcctacgt cggcgagctg
     3001 ctcggcgttc ccgccgtgga gagcgagccg cacccagata cccggaggcg tcgtcccctc
     3061 cagacgcagg attcccggat ggtccacgtc gatgcggaac cagtcgttgt cgccattcgg
     3121 gagcaggttg ccgcgcgtcc actcgttcag ttcgagcgga gaggccgtat ccggcgagtc
     3181 gttccgcccc tgggcgtcga gctgatcgca cggttcaaaa aacgtcccca ggatgtagga
     3241 cgacgggtcg gcgacgttgt cgttccactc ctttacctgg aggaatactg tcgcaggtcc
     3301 gtcagcgtgc c
//
LOCUS       DAOSWW010000195         3307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20816_length_3307_cov_3.166052, whole genome shotgun sequence.
ACCESSION   DAOSWW010000195 DAOSWW010000000
VERSION     DAOSWW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20816_length_3307_cov_3.166052"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="PK442_05130"
     CDS             complement(<1..1714)
                     /locus_tag="PK442_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOO87064.1"
                     /translation="MDILAGYAGKVLRVDLSSGAVSTELLTEEKLRPFLGGTGYAARI
                     LYDELKAGTDPLGPDNLFVLATGPLTDNLVPGGGSLSFCCKSPLTGGWAEARVGCDMG
                     GTLKKAGYDFVVLSGRAERPVHLVITDGRAELESAEHLKGRTVLEKNALLNEGGYSEY
                     DSLVIGPGGERLVLFASVMVGHRAAGRVGCGAVLGSKNVLAVSVRGSGKVVPADAAGY
                     MKAVREAHRIVKESPNTYGFTELGTMGGYEACDAMGDLPTKNWQSNSWGKGAQIASHF
                     VEENFTGGTGCYKGCPMKCGRKVRVKDGEWKTPEHDGGEYETIGSFTAYMLNEDVDCA
                     VHCGYLCNELGIDTISTGGAIGFLMECAEKGLLSPEMTQELDLSWGNASVLPKLVRMI
                     ALREGVGDILADGVRRAAERIGGGSEAFAVHVKGLEGPCHDPRAAKTLGVNYGTGNRG
                     MCHIHPLETVAYDASKFDFGLRPFGLQDPEELPRWEERGKGTAIRILQDAGALPEILG
                     TCKFYMYVGITPREYAEMLSALTGWNVTGEELMETAERATNLQRLFNIREGFSREDDM
                     LPGRV"
     gene            complement(1854..2312)
                     /locus_tag="PK442_05135"
     CDS             complement(1854..2312)
                     /locus_tag="PK442_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO87065.1"
                     /translation="MKKVVVPIDGSDTSKGVVDYAVYYANREHDANMLFLHVITVMEH
                     EPVFYGEGEVVLPPSDEKVTAEFTKFIEDRMEAAGMRIPRMSVSVRSGKVYEQIVRFA
                     EEQDADMIMIGHRGLSPMQRFFLGSVAAKVVAQAPCSVYVHRPKERRGKA"
     gene            complement(2505..>3307)
                     /locus_tag="PK442_05140"
     CDS             complement(2505..>3307)
                     /locus_tag="PK442_05140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOO87066.1"
                     /translation="TMDVWLSLLHPEDLGQVGDILAGHVRGELENYDHEVRMQHRDGH
                     WVWMHCKGRIISRTPDGEPLWMYGTYIDVTDRKQAEKELLEANRRLLAMQKVLKREAT
                     RDSLTGVLNRRAILEHLNEELARCRRDGTCLSAAICDIDHFKEVNDTYGHQTGDDVLC
                     GLVRLLRSNLREYDKIGRIGGEEFLLVLPGEAGRSAGFHERLVKSVAEHSIQTRSGAV
                     RITLSLGAITVSGDADTDEILARADKAMYKAKAEGRNRVVFISDAKAP"
BASE COUNT          551 a         1179 c          889 g          688 t
ORIGIN      
        1 gcacccttcc ggggagcatg tcgtcctccc tggaaaagcc ctcccggatg ttgaaaaggc
       61 gctggagatt cgtcgcgcgt tccgccgtct ccataagttc ctctcccgtg acgttccatc
      121 cggtaagcgc cgaaagcatc tccgcgtact cccgcggggt gatgccgacg tacatgtaga
      181 acttgcaggt ccccagaatc tccggcagcg cccctgcatc ctggaggatc cgaatcgccg
      241 ttccctttcc ccgctcctcc cagcggggca actcctccgg atcctggagg ccgaagggac
      301 gcaggccgaa atcgaacttc gacgcgtcgt aggccactgt ttcaaggggg tggatatggc
      361 acatcccgcg gttgccggtc ccgtagttca cgccgagcgt tttcgccgcc ctcgggtcgt
      421 ggcacggacc ttcaagcccc ttgacgtgga cggcgaacgc ctccgacccg cctccgatgc
      481 gttctgcagc ccggcggacg ccgtcggcga ggatatcccc cacaccctcc cggagcgcga
      541 tcatccgcac aagcttcggg agcacggagg cgttccccca ggagaggtcg agctcctgcg
      601 tcatctcagg cgagaggagc cccttctcgg cgcactccat caggaagccg atggcccctc
      661 ccgtggaaat cgtgtcgatt cccagctcgt tgcagaggta gccgcagtgg accgcacagt
      721 cgacatcctc gttgagcatg tacgcggtga acgagcctat ggtttcgtat tcgccgccgt
      781 cgtgctccgg cgtcttccac tccccgtcct tcacgcgcac ctttctgccg cacttcatcg
      841 ggcacccctt gtagcaccct gttccgccgg tgaagttctc ctcgacgaag tgagaggcaa
      901 tctgcgcacc cttcccccag gagttcgact gccagttctt cgtggggagg tcgcccatcg
      961 cgtcgcaggc ctcgtagccg cccatcgttc cgagctcggt gaaaccgtag gtgttggggc
     1021 tctccttcac gatgcggtgc gcctcgcgga cggccttcat atatccggcg gcgtctgcgg
     1081 ggaccacctt tccgctccct ctgaccgaaa cggcgagcac gtttttcgag ccgaggacgg
     1141 ccccgcaccc gacccggccc gcagcccgat gcccgaccat gacggaggcg aacagcacaa
     1201 ggcgttcgcc gcccgggccg atgacgagcg aatcgtactc cgaatagccg ccctcgttca
     1261 gcagcgcatt tttctccagc accgtcctgc ctttcaggtg ctccgcgctt tcgagctctg
     1321 cccgaccgtc cgtgatgacg aggtggacgg ggcgttccgc tcttccggag agaacgacga
     1381 agtcgtagcc cgccttcttc agcgttcctc ccatgtcgca cccgaccctg gcctcggccc
     1441 accctccggt caacggcgac ttgcagcaga aggagaggct tccccctccc gggacgaggt
     1501 tgtccgtcag cggccccgtc gcgaggacaa agagattgtc aggcccgaga ggatcggttc
     1561 cggccttcag ttcatcgtac agaatgcggg cggcgtaccc cgtccctccc aggaacgggc
     1621 gcagcttctc ctccgtcagc agctccgtcg aaacagctcc ggaggagagg tcgacgcgca
     1681 gcacctttcc ggcatatccg gcaagtatat ccattcaatc gtctcccttc ttctctcgtt
     1741 tctccatcct cctaccatac cacacgacgg gcggagagac accccgcccg tcgtttttta
     1801 tacattccgc gtcgaaagaa caggcatccc ttccggcccg cctgtcgggt tcctcatgct
     1861 ttgccccgcc gctccttcgg ccggtggacg tagacgctgc agggcgcctg cgccaccacc
     1921 ttcgcggcca cgctcccgag gaagaagcgc tgcatcggac tcagccccct gtggccgatc
     1981 atgatcatat cggcatcctg ctcctcggca aaacggacga tctgctcata gaccttgccc
     2041 gagcgcactg aaacggacat tctcggaatc ctcattcccg ccgcttccat ccggtcttcg
     2101 atgaacttcg tgaattccgc cgttaccttc tcgtcggacg ggggaagcac cacctccccc
     2161 tcgccgtaga agacgggctc gtgttccatg acggtgatca catgaaggaa gagcatgttc
     2221 gcgtcatgct ctctgttcgc gtagtatacg gcgtagtcca cgaccccttt gctggtatcg
     2281 ctgccgtcga tgggtaccac aacttttttc atccgcaacc cctcctttac gcccgtttcg
     2341 ggcatccgaa ctccagtccc gtcagcgccg gaaggtcttg agtgaagtat agcattcccc
     2401 agtctattta gtcaatttta cgacaacttt tccctctcct gctcaaaaag ctctccagcg
     2461 tcctcccgaa cggccgatac atcttgctcc cggaaagcgc ctgttcaggg agccttcgcg
     2521 tcgctgatga agaccacccg gttgcgcccc tccgccttgg ctttgtacat cgccttgtct
     2581 gcgcgcgcaa gaatttcgtc cgtgtcggcg tcgccggaaa cggtgatcgc tccgaggctc
     2641 agcgtaatgc gcacggctcc cgaacgggtc tggatggagt gttccgcaac gcttttcacc
     2701 agccgttcat gaaaccccgc gctccgcccc gcctctccgg ggagaaccag caggaattcc
     2761 tccccgccta ttcttccgat cttgtcgtac tccctgagat tgctccgcag cagccggacc
     2821 agcccgcaca ggacgtcgtc gcccgtctga tgcccgtacg tatcgttgac ctccttgaag
     2881 tggtcgatat cgcatatcgc cgcgctcagg cacgttccgt cccgccggca acgcgccagt
     2941 tcctcattca gatgctccag tatcgctctc cgattgagca cgcccgtcag ggagtcgcgc
     3001 gtcgcctccc gtttcagcac cttctgcatc gccagaagcc tgcggttggc ctccagaagc
     3061 tccttttccg cctgtttccg gtctgtgacg tcgatatagg tgccgtacat ccacaggggt
     3121 tcgccgtccg gcgttcgcga gatgatccgc cccttgcagt gcatccagac ccaatgcccg
     3181 tccctgtgct gcatccggac ctcgtggtcg tagttctcca gttctccccg cacgtgcccg
     3241 gcgaggatat ccccaacctg ccccaggtcc tcggggtgca gcaaactcag ccacacatcc
     3301 attgtga
//
LOCUS       DAOSWW010000196         3301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_20875_length_3301_cov_3.894023, whole genome shotgun sequence.
ACCESSION   DAOSWW010000196 DAOSWW010000000
VERSION     DAOSWW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20875_length_3301_cov_3.894023"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PK442_05145"
     CDS             <1..866
                     /locus_tag="PK442_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO87067.1"
                     /translation="ADASGSIVTRHTVRDAVSSFDGGRARILLAEDNITNQHVALGIL
                     KKFGLRADAVADGEEALAALSAVPYDLVLMDCQMPRMDGYEAARRVRMMEGAVRDVPI
                     IAMTASAMAGDREKCLEAGMNDYVPKPVSPGVLAGALKKWLPSRGERGGAREASSKES
                     AANGSPVLQSKEQNAESRPWNREAFLERLRGDHELAAEIVGDYLSEIPKEIDALREAL
                     ATGDAEAGERCAHSVKGVSASVGGERVRDVAFRMERAAAAGDLEMARASLPRLEEEFE
                     LLKDAMKRSGH"
     gene            980..2599
                     /locus_tag="PK442_05150"
     CDS             980..2599
                     /locus_tag="PK442_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO87068.1"
                     /translation="MRKGIAAVLGIALLLSLSGAAALAAPREVVIGMPGDVHSMDPYT
                     LNETINNAINNHLFDTLVETDNNLQPKPGLAESWEVSEDARTWTFHLRKGVTFHNGNP
                     FTVDDVIYSFDRSKDREVGASFVHVLSTVESYEKIDDHTLKVTCKSPNALLLAHFKDL
                     LILNKAHCEGQGNDWIALNPNGTGRYKLVEHVRGDRLVLERNPAFWGEQPIVEKVTFK
                     PITNEGTRTANMLSGAVDLVVNVPVRDVKMLEKNKNVTVIAQPGLRAIYLNMAGWSDT
                     PSKDAKMPLKSPDGSNPFKDIRVREAMYRAINIDEIVEKIMNGFAKPMATYSPEGYNG
                     FNPDVKRLAYDPALAEKLLDEAGYKRQSDGYRFEVTLDAPNDRYINDGPVATAIAGYF
                     EKVGIKVNPNLMSRSVFFSYISTTNKAGDNTHLCMTGWSDSGGESALIGLDMLYSITQ
                     DGEVKEGYGGVNRGFYCNPEVDKLIDEAIATGDPAQRGEIMKKAWQLAADDVSYIPLY
                     FEVDLYAHGKKITYTPRKDKYVFAWDVSFND"
     gene            2724..>3301
                     /locus_tag="PK442_05155"
     CDS             2724..>3301
                     /locus_tag="PK442_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87069.1"
                     /translation="MFRFIVKRLFQLVLVLFAVSLIVFLFTSVMGNPVYLMVKENAPA
                     ADIQAVTAALGLDKPLPVQYGIFIKNALQGNFGKSYMNHLPALGLIVERFPATLEIVV
                     VALLLSACIGIPCGVISGAYPKSRFSQVIMAFSIAGISMPSFWIGMVLIYYFSLHLGV
                     LPVSGRGAVGTILGIKTSLATLDGWQHIILPSV"
BASE COUNT          696 a         1022 c         1000 g          583 t
ORIGIN      
        1 atgcggacgc ctcgggctcc atcgtcaccc gccacacggt gcgggatgcc gtctcctcct
       61 tcgatggagg aagggcgcgc attctgcttg cggaggacaa catcaccaac cagcacgtcg
      121 ccctcggcat actcaagaag ttcggcctga gagcggacgc ggtcgccgac ggagaagagg
      181 cgcttgccgc actctcggcc gtaccgtacg atctcgtcct gatggactgc cagatgccgc
      241 gcatggacgg gtacgaagcc gcgcggagag tccgcatgat ggagggggcg gttcgtgacg
      301 tgccgatcat cgccatgacg gcgagcgcca tggcggggga cagggagaag tgcctggagg
      361 ccggaatgaa cgactacgtt cccaagcccg tttcgccggg cgtccttgcc ggagcgctga
      421 agaaatggct gccgtcccgc ggtgaacgcg gcggcgcccg ggaggcttcg tcaaaagaga
      481 gcgcggcgaa cggctctccc gtactgcaaa gcaaggagca gaatgccgag tcgcgtccgt
      541 ggaacaggga ggccttcctg gaacgcctgc gaggagacca cgagctggcg gcggagatcg
      601 tgggcgatta tctctccgag attccgaagg agatcgacgc gctccgggag gccctcgcaa
      661 cgggagatgc ggaagccgga gagcgctgtg cgcacagcgt gaaaggcgtc tccgccagcg
      721 tcggcggcga aagagtccgc gatgtagcct tccgcatgga gagggccgcg gctgcgggcg
      781 acctggagat ggcaagggcg tctttgcctc gcctggaaga agaattcgaa ctgctgaaag
      841 acgcgatgaa acgatcggga cactgaaact gtcgaaaaag tgcgattgac tttgccttcg
      901 gagcactata aaatgtatcc gcaaaggtat acttagcctt gcattcggaa tgtcaccaca
      961 gaaaagggag gaatgtatcg tgaggaaagg aatcgccgca gtactcggaa tcgccctgct
     1021 gctctcgttg tcgggagcgg ccgcgctcgc agcgccccgg gaggtcgtga tcggcatgcc
     1081 gggggatgtt cactcgatgg acccctacac gctgaacgaa acgatcaaca acgccatcaa
     1141 caatcatctc ttcgacacgc tggtcgagac ggacaacaat ctccagccca agcccggcct
     1201 tgccgagagc tgggaagtct ctgaggacgc ccgcacctgg acctttcacc tcagaaaggg
     1261 agttaccttc cataacggca atcccttcac cgtcgacgac gtcatctact ccttcgaccg
     1321 ttccaaggac agagaggtcg gcgcttcctt cgtgcacgtg ctctccactg tggagagcta
     1381 cgagaagatc gacgaccata cgctgaaagt cacctgcaaa tcgcccaacg ctttgctgct
     1441 ggcgcacttc aaggatctgc tgatcctgaa caaggcgcac tgcgaaggcc aggggaacga
     1501 ctggatcgcg ttgaacccca acggcaccgg ccgctacaag ctggtggagc acgtgcgcgg
     1561 agaccggctc gtgctggagc gcaaccccgc cttctggggc gagcagccga tcgtcgaaaa
     1621 ggtcacgttc aagccgatca ccaacgaggg aaccaggacc gccaacatgc tttcgggcgc
     1681 ggtcgacctg gtcgtcaacg tccccgtgcg cgacgtgaag atgctggaga agaacaagaa
     1741 cgtcaccgtc atcgcccagc cggggctgcg cgccatctac ctcaatatgg cggggtggag
     1801 cgacacgccg tcgaaggatg caaagatgcc gctgaagtcc cccgacggga gcaatccgtt
     1861 caaggatatc cgcgtccgcg aggcgatgta ccgcgccatc aacatcgacg agatcgtcga
     1921 gaagatcatg aacggcttcg cgaagccgat ggccacctac tcgcccgaag ggtacaacgg
     1981 attcaatccg gatgtgaagc gccttgcgta cgaccccgcg ctcgcggaga agctgctcga
     2041 cgaggccgga tacaagcggc agagcgacgg ctaccgtttt gaagtcaccc tcgacgcccc
     2101 caacgaccgc tacatcaacg acggtcccgt ggccacggcg atcgcgggct acttcgagaa
     2161 ggtcggcatc aaggtcaacc cgaacctcat gtcccgctcg gtcttcttct cctacatctc
     2221 caccaccaac aaggcggggg acaacacgca cctctgcatg accggctggt ccgattcggg
     2281 cggcgagagc gcgctcatcg ggctcgatat gctctacagc atcacccagg acggagaggt
     2341 caaagaaggg tacggcggcg tcaacagggg cttctactgc aaccccgagg tcgacaagct
     2401 gatcgacgag gcgatcgcca ccggcgatcc ggcgcagcgc ggcgaaatca tgaagaaggc
     2461 ctggcagctg gccgccgacg acgtctccta catccccctc tacttcgagg tggacctgta
     2521 cgcccacggc aagaagatca cctacacgcc ccgcaaggac aagtacgtct tcgcgtggga
     2581 cgtctccttc aacgactaag acgaatgtgc gacgaggttc ccggcgcgcc ctatccggcg
     2641 ccgggaattt ttgaaaagag cggcggacct tgctctgacg gagggtccgc cgtattctgt
     2701 accataggta tggaggtgga accgtgtttc gtttcatcgt caaaagattg tttcagctcg
     2761 tcctcgtgct cttcgcagtc tcgctcatcg tctttctctt caccagcgtc atggggaatc
     2821 cggtctatct catggtcaag gagaacgctc ccgccgcgga tatccaggcg gtcacggcgg
     2881 ccctcggact ggacaagccg ctccccgtgc agtacggcat atttatcaag aacgcgcttc
     2941 aggggaactt cggcaagtcg tacatgaacc accttcccgc cctcggcctg atcgtcgaac
     3001 gcttcccggc cactcttgaa atcgtcgtcg tcgcgctgct tctgtcggcg tgcataggga
     3061 ttccctgcgg cgtcatatcg ggcgcttatc cgaagagccg cttcagccag gtcatcatgg
     3121 ccttttcgat cgcgggcatc tccatgcctt cattctggat cggcatggtg ttgatctact
     3181 atttcagcct tcaccttggc gtcctcccgg tttcgggacg aggcgccgtg ggaacgatcc
     3241 tgggaatcaa gaccagcctc gccacgctcg acggctggca gcacatcatc cttccctcgg
     3301 t
//
LOCUS       DAOSWW010000197         3283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21078_length_3283_cov_2.932776, whole genome shotgun sequence.
ACCESSION   DAOSWW010000197 DAOSWW010000000
VERSION     DAOSWW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21078_length_3283_cov_2.932776"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PK442_05160"
     CDS             complement(<1..1149)
                     /locus_tag="PK442_05160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO87070.1"
                     /translation="MSEMLKKYGEVVGFHAMDSLMVIADMLRGKKIVHVNSTKEGGGV
                     AEILSSMVPLSKELGLDVSWEVIEGNPDFFACTKTLHNSLQGMPGGLPEQQIEAYVET
                     NRTNAEKLRGVVEDADYVFIHDPQPAPLLGYFPKRKGKWIWRCHIDVSSPNRSVWRFV
                     KKHVEGYDASIFSLPDFAQTLPHPQFLITPSIDPLSEKNIALPKSEVAKTYEELGIPM
                     DKPVILQVSRFDRFKDPLGVIESFRIAREHVDIRLVLAGGGATDDPESGEVLARVQEA
                     AAGDPDIHVLLLPADAHRTINALQSGADVVMQKSTKEGFGLTVTEGLWKKKPVIGGDV
                     GGIRLQIINRFNGFRVRSPEGAAIRIRYLLTHKWKRERMGVNARQYVLE"
     gene            complement(1152..1826)
                     /locus_tag="PK442_05165"
     CDS             complement(1152..1826)
                     /locus_tag="PK442_05165"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HOO87071.1"
                     /translation="MKEHGTTLPFRFMECALLTLSTGVRAQSIRELRTALPQTPLSSI
                     YYHFWGRMLRPHIAESEFNNDFASWADSGLGDIELAEALSAINPVACADLGEIRSILE
                     DILDARLENGDFMSWKKSDREFYFIACTKMMFDTGREVSSLKDFPLAVKTASRQSFFH
                     HFIDGRRRSPEGKDDFSLWLEQFGEETEPLRKSLRAIDSYLFSLGELKGQVVSLLEKN
                     LHRRGK"
     gene            complement(2004..2873)
                     /gene="secF"
                     /locus_tag="PK442_05170"
     CDS             complement(2004..2873)
                     /gene="secF"
                     /locus_tag="PK442_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOO87072.1"
                     /translation="MKIMFIDFMKHRRAALLVSLVCVVVSIGLVFTKGLNLGIDFTGG
                     NVVQVEFAQGVSVGDVRDVLASVGQGNAVIQSYSDIGFIIRISANEDEARKMVVDALR
                     AKYPDMKTVRLEKVGPVVGAELRKEAFVALFIALAGILAYITVRFQFRFAVVSVLALM
                     HDAIITLGVFSLTGMEISSSFIAAILTIVGYSLNDTIVVLDRVRENWKTLRGNGMLAL
                     LNNSINETISRTINTSMTTLLPVIALFLWGGPVLRSFSFALLIGITVGTYSSIYIASG
                     VLAEWWIKAPQKA"
     gene            complement(2893..>3283)
                     /locus_tag="PK442_05175"
     CDS             complement(2893..>3283)
                     /locus_tag="PK442_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOO87073.1"
                     /translation="VLLVGLMSLFEATLTLPGIAGIVLTIGMAVDGNVLIYERMKEEL
                     RAGKTPLAAIDAGFKKALVTIFDANITTLIAAAVLFYFGSGPVRGFAVTLSIGIVSSV
                     FCNVLVTRSLLQYFMGGRATSSLAHRS"
BASE COUNT          686 a          985 c          923 g          689 t
ORIGIN      
        1 ctcgaggaca tactgccgtg cgtttactcc catgcgctcc cgcttccact tgtgtgtgag
       61 aagataccgg atacgaatgg ccgcgccttc gggggagcgc acgcggaacc cgttgaaacg
      121 gttgatgatc tgcaggcgta ttccgccgac gtcccctccg ataaccggct ttttcttcca
      181 caagccttcg gtcactgtaa ggccgaagcc ctccttggtc gatttctgca tcactacgtc
      241 ggcgccggac tgcagggcgt tgatcgtcct gtgcgcatcg gctggaagaa gcaatacgtg
      301 gatatccggg tcgcccgccg ccgcctcctg aaccctggcg agcacctcgc ccgattcggg
      361 gtcgtcggtc gccccgcccc cggcgagcac aaggcgaata tccacgtgct cgcgggcaat
      421 tctgaagctt tcaataaccc cgagggggtc cttgaagcgg tcgaaccgcg agacctgaag
      481 gatgaccggt ttatccatag ggataccgag ttcctcgtag gtcttcgcaa cctcgctttt
      541 cggcagcgcg atattctttt cgctcagggg gtcgatgctc ggcgtgatca gaaactgggg
      601 gtgcgggagc gtctgggcga aatcgggaag cgagaagatc gacgcgtcgt acccctccac
      661 gtgcttcttg acaaaccgcc agaccgagcg gttcggcgag ctgacgtcga tatggcagcg
      721 ccaaatccac tttccctttc tcttcggaaa atacccgagc agcggcgcgg gctgcggatc
      781 gtggataaag acataatctg catcctcaac cacgccccgc agcttctccg cgtttgtacg
      841 gtttgtctcg acgtacgctt cgatctgctg ttccggcagt ccccccggca tcccctgcaa
      901 agagttatga agggtttttg tacaggcgaa aaagtcggga ttcccttcga tgacctccca
      961 ggagacgtcg agtccgagct ccttcgagag cgggaccatc gaggagagaa tctccgccac
     1021 gcctcccccc tctttcgtcg agttcacgtg gacgattttc ttcccccgca gcatatccgc
     1081 gatgaccatc aggctgtcca tcgcatggaa gccgacaacc tcgccgtatt ttttcagcat
     1141 ctcggacatc gttatttccc cctcctgtgc agattttttt caagaaggga aacaacctgc
     1201 cccttcaact caccgagtga gaagagatac gaatcgatcg ccctgagcga cttccgaagc
     1261 ggctccgtct cttcgccgaa ctgctcgagc cagagagaaa agtcgtcctt cccctcgggg
     1321 gagcgtctcc tgccgtcgat gaagtgatga aagaagctct gacggctcgc cgtcttcacc
     1381 gcaagcggga agtccttcag cgaggagacc tcgcgccccg tgtcgaacat catcttggtg
     1441 caggcgataa agtagaattc ccggtcgctc ttcttccagc tcataaaatc gccgttctca
     1501 agacgcgcgt ccagaatgtc ctcaaggatc gagcgtatct cgccaagatc ggcgcacgct
     1561 acggggttga tcgcgctgag cgcctcggcg agctctatat ctccaaggcc gctgtcggcc
     1621 cacgaggcga agtcgttgtt gaactcggat tcggcgatat gggggcgcaa catcctcccc
     1681 cagaagtggt agtagatgga actgagcggc gtttgcggaa gcgcggttcg aagctctcgt
     1741 atggattgcg cgcgcactcc ggtactcagc gtgagcagag cgcattccat gaatctgaac
     1801 ggaagtgttg ttccgtgttc tttcatttct gtttcctcct tttcccggcc gatctcatct
     1861 ccttttatgt tgtggccatg acattattgt aagagtataa cacataaacg ccttgttcta
     1921 caaattctca tgcccaaaaa agcgctcgcc actgttctgg cgagcgcttc gtaatgacaa
     1981 tactccaaaa agcagaacag cggctacgcc ttctgaggcg cctttatcca ccattcggcg
     2041 agcacgccgg aggcgatgta aatggagctg tacgtgccga ccgttatgcc gatgagcaga
     2101 gcgaacgaga agctgcgcag cacagggcct ccccagagga agagcgcgat gacaggcagc
     2161 agggtcgtca tggacgtgtt gatcgttctg gagatcgttt cattgatcga attgttcagc
     2221 agggcgagca tcccgtttcc gcgcagcgtt ttccagttct cgcggaccct gtcgaggacg
     2281 acgatcgtgt cgttcaggga gtagccgacg atcgtgagga tcgccgcgat aaacgacgag
     2341 gagatctcca tgccggtaag actgaagacg ccaagcgtga tgatcgcgtc gtgcatcagc
     2401 gcgaggacgc ttaccacggc aaaacggaac tggaagcgga ccgtgatata cgcaaggata
     2461 ccggcaagcg cgatgaagag cgccacgaag gcctctttcc gcagctcggc gccgaccacg
     2521 ggtccgacct tttcaagccg gacggttttc atatcgggat actttgcccg aagggcgtcg
     2581 acaaccattt tcctggcttc gtcctcgttg gcgctgatcc ggatgatgaa gccgatatcg
     2641 ctgtaggact ggataacggc gttcccctgg cccaccgaag ccaggacgtc gcggacatcg
     2701 cccacggaga cgccctgcgc gaactcgacc tgcaccacgt tgcctcccgt gaaatcgatt
     2761 cccaggttca gtcccttcgt gaagaccaga ccgatgctga cgacgacgca gacaaggctg
     2821 accaggagcg cagccctgcg atgtttcata aagtcaatga acatgatttt catagtcttt
     2881 tctccacctc tcttaggaac ggtgcgccag ggagctcgtc gcccgtccgc ccataaagta
     2941 ctggaggaga gagcgcgtga cgaggacgtt gcagaagacg ctcgacacaa taccgatgct
     3001 gagcgttacc gcaaaacccc tgacagggcc gctgccgaag tagaagagga ccgccgccgc
     3061 gatgagcgtc gtgatattcg cgtcgaagat cgtcacgagg gcctttttga atccggcgtc
     3121 gatggccgcg agcggcgtct tgccggcgcg cagctcctcc ttcatgcgtt catagatgag
     3181 cacgttgccg tccaccgcca tgcctatggt aagcacgata cccgctatgc cgggcagggt
     3241 cagcgtcgcc tcgaagagcg acatcagacc cacgagcagc acc
//
LOCUS       DAOSWW010000198         3278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21130_length_3278_cov_3.583928, whole genome shotgun sequence.
ACCESSION   DAOSWW010000198 DAOSWW010000000
VERSION     DAOSWW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21130_length_3278_cov_3.583928"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PK442_05180"
     CDS             <1..966
                     /locus_tag="PK442_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO87074.1"
                     /translation="LMGKGSFPETHPLALGMAGMHGTPGANVAMSQADVILAVGARFS
                     DRTTGKVADFAKNACVIHIDLDDAEIDKIVPCAVPLVGDAGAVLALLADALPEVTWRE
                     WTDRLREQVEEMPLLRPGETDFVPGAIFEAVRRRADEKEIAVTDVGQNQMWAALFWKT
                     EHPRTFLSSGGLGTMGYALPAAIGASLAHGKAPVLCFAGDGGFLMNIQELETCARYQI
                     PVKIFLLNNGCLGMVRQWQELFWGERYAATTQNPVCNFPALAEAFGVQGRACETLDDL
                     ESALDDLFETPGPALVDCRIPQEELVMPMVPAGTALKDFMYRVRV"
     gene            1074..1349
                     /locus_tag="PK442_05185"
     CDS             1074..1349
                     /locus_tag="PK442_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOO87075.1"
                     /translation="MPIIKPISDLRNNFAAISETCHREGEPVFITRNGEGDLVVMSMA
                     AYEQREATLDLYAKLLEARERKARGEASVARADAMSELRARVRGKKA"
     gene            1333..1650
                     /locus_tag="PK442_05190"
     CDS             1333..1650
                     /locus_tag="PK442_05190"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO87076.1"
                     /translation="MGKKHSVLLLSAAMDDLRGIADYVARDDEERALEVIDEIDAALD
                     RLEDFPESGAVPRDAALARMGYRVLVVSSFLVFYTIEEHVVEVHRVIHGRRRYAHLFG
                     NGA"
     gene            1725..2120
                     /locus_tag="PK442_05195"
     CDS             1725..2120
                     /locus_tag="PK442_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO87077.1"
                     /translation="MTTAIERTLTDFFEEEQSVRLAFLMGSFAKETARPDSDVDVAAL
                     FDRTPDFMETLDLRERLSALLRREADLVVLNDAGPIIGMQALKTGIRLFARKNEYEEF
                     FVRTLNEYDDLKRLRAPIEEAVLKRRIYA"
     gene            2113..2310
                     /locus_tag="PK442_05200"
     CDS             2113..2310
                     /locus_tag="PK442_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87078.1"
                     /translation="MPDKDVVLAKVATIQRCLARIRDVTSLDPARLYDGYRAMAMDAE
                     REKEAAEWCDALSRGATDEAR"
     gene            complement(2328..>3278)
                     /locus_tag="PK442_05205"
     CDS             complement(2328..>3278)
                     /locus_tag="PK442_05205"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOO87079.1"
                     /translation="KIPTLSFYEYCDLLQLPERPRLEAGIQPTRLHTMDKGDFSHLMA
                     GLSPLQRHFNRYLTVGGFPELALSQDDAFSQRMLREDVVDKVIKRDILALFNVRNPLQ
                     LEKVFLYLCMHSSGIVNMTTMSKELDGMNKSTLTNYIQFLKEANLIYISDPVGVDGKG
                     VLKGRPKIYVADAAIRSAVLMLENAIADAGETGIMVETTVYKHIAAFYSAANARVGYY
                     RRVKDGEKEVDVVVELPAGRILCEVKYRENTHVKGTDAIIELADDERENVLGALIITK
                     RAEDYGVAAHATRTPVVRIPAPAFLYLLGNAEKEGDAAKM"
BASE COUNT          649 a         1007 c         1007 g          615 t
ORIGIN      
        1 ctgatgggca agggcagctt ccccgagacg cacccgctcg cgctcggcat ggcgggaatg
       61 cacggcacgc ccggagcgaa cgtcgccatg tcgcaggccg acgtcatcct cgccgtcggc
      121 gcccgcttca gcgaccgcac caccggcaag gtggccgact tcgcgaagaa cgcctgcgtc
      181 atccatatcg acctcgacga cgcggagatc gacaagatcg ttccgtgcgc cgtcccgctc
      241 gtcggcgacg ccggcgccgt gctcgcactc cttgcggacg cgctgccgga agtaacctgg
      301 agagagtgga ccgaccgcct ccgcgagcag gtcgaagaga tgccgctgct ccgccccggc
      361 gagaccgact tcgtccccgg cgccatcttc gaggccgtcc ggcgcagagc cgacgagaag
      421 gagatcgccg tcaccgacgt ggggcagaac cagatgtggg ccgcgctctt ctggaaaacg
      481 gagcacccgc gcacgttcct gagttcgggc gggctgggga ccatggggta cgcccttccg
      541 gcggccatcg gcgcctccct cgcgcacggc aaagccccgg tgctctgctt cgccggggac
      601 ggcggcttcc tgatgaacat ccaggagctg gagacctgcg cgcgctacca gattccggtg
      661 aagatcttcc ttttgaacaa cggctgcctc ggcatggtcc gtcagtggca ggagctcttc
      721 tggggcgagc gctacgccgc caccacgcaa aatcccgtct gcaacttccc cgccctcgcg
      781 gaagccttcg gcgtccaggg gcgcgcctgc gaaacgctcg acgacttgga gagcgccctc
      841 gacgacctct tcgagacgcc cggtccggcg ctggtcgact gccgcatccc gcaggaggag
      901 ctggtcatgc ccatggtccc ggcgggaacg gcgctgaagg attttatgta tagggtgagg
      961 gtgtaagaga accgcaaatc aaacacagcg cgccgcagcc tccggatggg gggctgcggt
     1021 ttttctcatg ataatgatat aatacgatca ttattgtgaa agagggtgtc cgcatgccga
     1081 tcataaagcc gatttcggac ttacggaaca acttcgccgc gatctccgaa acctgtcatc
     1141 gggaaggaga gcccgtgttc atcaccagaa acggcgaagg cgatctcgtg gtgatgagca
     1201 tggccgcgta cgaacagcgc gaggccacgc tcgatctcta cgcgaagctt ttggaggcgc
     1261 gggagcgcaa agcgcgcggc gaagcctccg tagcgcgcgc cgatgcgatg agcgagctga
     1321 gagcgcgggt ccgtgggaaa aaagcatagc gtccttctcc tctcagccgc gatggacgat
     1381 ctccgcggca tcgcggacta cgtcgcccgc gacgatgaag agcgcgccct cgaagtgatc
     1441 gacgaaatcg acgccgcgct cgaccggctc gaagacttcc cggaaagcgg cgccgtcccg
     1501 cgcgatgcag cgctggcgag gatgggctac agagtgctcg tcgtgagcag ctttctggtt
     1561 ttctatacga tagaagaaca cgtagtggaa gttcacagag tgattcacgg acgccgccgc
     1621 tacgcccatc tgttcgggaa cggagcgtag agtcagactg ccgcgaacgc tttgtgctat
     1681 gcttctatgg aaatggagat ttttcggtaa agaggcgata cgacatgacg acggccatcg
     1741 agcgaacgct cacggacttc ttcgaggagg agcaatccgt ccgcctcgct ttcctgatgg
     1801 gttccttcgc caaggagacg gcccgtccgg acagcgatgt ggacgtcgcc gcgcttttcg
     1861 accggactcc ggacttcatg gagacactcg atctgcggga gcggctttcg gccctgctcc
     1921 ggcgcgaggc cgatctggtg gtgttgaacg acgcgggacc gatcatcggg atgcaggcgc
     1981 tgaagaccgg gatcaggctc ttcgcccgga aaaacgagta cgaggagttc ttcgtccgca
     2041 cccttaacga gtacgacgac ctgaagcgcc tccgcgctcc gatcgaagaa gccgtgctga
     2101 agaggagaat ctatgcctga caaagacgtg gtgttggcaa aagtcgcgac catccaaagg
     2161 tgtctggcgc ggatacgcga cgtcacgtcg ctcgatccgg cacgtctcta cgacgggtac
     2221 agggccatgg caatggatgc ggagcgcgaa aaagaggcag cggaatggtg cgacgcgctt
     2281 tcgaggggcg cgaccgatga agcacggtaa gcgctgaaag cgagactcta catcttcgcg
     2341 gcatcccctt ctttttcggc gtttccgagg aggtagagaa atgcgggggc gggtatgcgc
     2401 acgacgggcg tccgcgttgc atgggcggcg actccgtaat cctcggcccg tttcgtgatg
     2461 atcagcgctc ccagtacatt ctcgcgctcg tcgtccgcga gttcgatgat cgcgtccgtc
     2521 cctttgacat gcgtattctc gcggtatttc acttcgcaca gtatccgacc cgccggaagc
     2581 tccaccacca cgtccacctc tttttctccg tccttgaccc tcctgtagta gccgacgcgc
     2641 gcgttcgccg cggaatagaa cgcggcgatg tgcttataga ccgttgtttc caccatgatc
     2701 cccgtctccc cggcgtcggc gatcgcgttc tccagcatca gcacagcgct tcgtatagcg
     2761 gcatcggcca catagatctt cggtcgcccc ttcagtacac ccttcccgtc gacacctacg
     2821 ggatcgctga tatagatcag gttcgcctct ttcaggaact gaatatagtt cgtcaacgtg
     2881 ctcttgttca ttccgtccag ctccttggac atcgtcgtca tgttcacgat gccggaagag
     2941 tgcatgcaga ggtacaagaa gactttttcg agctggagag ggttccgtac attgaaaaga
     3001 gccagaatat cccgtttgat caccttgtcg acaacatcct cgcgcagcat cctctgagaa
     3061 aaggcgtcat cctgcgacag cgcaagttcc ggaaagccgc cgaccgtcag gtacctatta
     3121 aagtgacgct gaagcggcga aagtccggcc atcagatggc tgaaatcgcc cttgtccatc
     3181 gtgtgcagcc tcgtcggttg aatacccgct tcaaggcggg gccgctcggg gagttgcagc
     3241 agatcgcagt actcgtaaaa ggaaagcgtc gggatctt
//
LOCUS       DAOSWW010000199         3267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21220_length_3267_cov_3.749689, whole genome shotgun sequence.
ACCESSION   DAOSWW010000199 DAOSWW010000000
VERSION     DAOSWW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21220_length_3267_cov_3.749689"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PK442_05210"
     CDS             complement(<1..778)
                     /locus_tag="PK442_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87080.1"
                     /translation="MSEVILKNVTKKFSTRTLGEVTAVDDFNLTIREGECFSLLGPSG
                     CGKTTTLRMIAGFEDLSDGEIWIGDKPVSAKKRNLYVQPEDRGLGMVFQAFAVWPHLN
                     VFENVAFPLRVRNVGRREMDSRVAEALKHTGLGGLDRTFPSDLSGGQLQRIALARAIV
                     MNPKVLLLDEPLSNLDPKLRESMRFEIKELQRRFNFTIIYVTHDQAEAMALSDRMLVM
                     DMGVIQQIDTPTNMYNNPANKFVYGFLGLSNFVDVNISDGK"
     gene            complement(775..2475)
                     /locus_tag="PK442_05215"
     CDS             complement(775..2475)
                     /locus_tag="PK442_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO87081.1"
                     /translation="MKMFGRREGKLDLNQILMLICLTILIITVAMPMIMIVYNTFFYG
                     FTFDWSMFTNVVMDPSNVDAMWNTIVIAFWVTVVGTIVGVFYAWLIGRTNIPLKGLMK
                     TLFVIPYMFPPFIGAMAWGLLLSPRSGYINKLWMLMMGTREPLMNINSIAGIVFVEVC
                     YYFPFVFLQVVSALERMDPTLEESARISGANQLYVIRKITLPLVVPAVAAGAMLILIS
                     SLSHFGVPAMLGFSRGIFTLPTKIYELINRSAGSFEGIRQGAALSILLVVVVVIALWL
                     QRKILKAGHYDIIRGKSMRPMLINLRTSKFPLLGVCLASLFVIVILPLYIIFAVGGLK
                     AYGLPLNLANMTLDNYYHVLFSSRMAQDAIKNSFFLAISSAIIAMFVGTMIAYVVVKV
                     KVKGKSLLEILALLPYSVPGIVLAIGVILMWSGTFYLNLYNTIWIILIAYIARYVAFA
                     MKSSSASLEQVHYSLEEAARSCGATHLESLRDITIALIRPGMIAGFFLIFLPAMRELT
                     TSILLYGPFSRTLGVAIYAINAEGNITQSAALSGIAILIIMLGNLALRFVTKDRRSVS
                     "
     gene            complement(2472..>3267)
                     /locus_tag="PK442_05220"
     CDS             complement(2472..>3267)
                     /locus_tag="PK442_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87082.1"
                     /translation="FENVYYPLKKRKVPKDEAKKRALDAIEQVRMKGYEQRMPAQLSG
                     GQQQRVALARALVSSNQLMVLDEPISNLDANLREEMRFEIKELQRRTGITVIYITHDQ
                     EVALAIADRMAILDRNGYIRQIGVPEDVYRNPKDSYIYKFLGVSNFIPVERRGQEYVL
                     DGTEGKQAFPYEIPEALRAERLFAACRPVDITVGGDGPVQGFVKHVVYLGNLFEYRVL
                     LGGREIRVQQDSHEAFRAGVPEEGRPCALSFRNLRFYQEDEGGPAS"
BASE COUNT          699 a         1028 c          927 g          613 t
ORIGIN      
        1 ccttcccgtc gctgatgttc acgtcgacga agttggagag gcccaggaag ccgtagacga
       61 acttgttcgc cgggttgttg tacatgttcg tcggcgtgtc gatctgctga atcacgccca
      121 tatccatcac cagcatcctg tcggagagcg ccatcgcctc cgcctgatcg tgcgtcacgt
      181 agatgatggt gaagttgaat cgccgttgca gctccttaat ctcgaagcgc atcgactccc
      241 gaagcttggg gtcgagattc gacagcggct cgtcgagcag cagcaccttc gggttcatga
      301 cgatggcccg ggcgagcgcg atccgctgga gctgccctcc ggacaggtcc gagggaaacg
      361 tccggtccaa accgccgagt ccggtatgtt tgagggcctc cgcgaccctg gaatccatct
      421 ccctcctccc gacgttccgc acccgcagag ggaaggcaac attttcgaag acgttcagat
      481 gcggccaaac ggcgaacgcc tggaagacca tccccagccc ccggtcctcc ggctgcacgt
      541 agagattgcg cttcttcgcg gacaccggct tgtcgccgat ccagatctct ccgtcgctga
      601 gatcctcgaa ccccgcgatc atccggagcg tggtcgtctt tccacatccc gacggcccca
      661 acagggagaa gcactcgccc tccctgatcg tcaaattgaa gtcgtcgacc gcggtcacct
      721 ctcccaaagt cctcgtggag aatttcttcg tgacgttctt caggataacc tcgctcatga
      781 gacgctcctc ctgtccttgg taacaaaccg gagcgccagg ttaccgagca tgatgatcag
      841 tatggcgata ccggacagcg ccgccgactg ggtgatgttc ccctcggcgt tgatggcgta
      901 gatcgccacg cccagcgtgc gggagaacgg cccgtagagc agaatcgagg tggtcagctc
      961 cctcatggcc ggcagaaaga tgaggaaaaa tcccgcgatc atacccggcc gtatcagcgc
     1021 gatggtgatg tcccggagcg attcgaggtg cgtcgcgccg caagagcggg ccgcctcctc
     1081 aagagagtag tgcacctgct ccagcgaggc ggacgaactc ttcatcgcga acgccacgta
     1141 ccgcgcgata taggcgatca ggatgatcca gatggtgttg tacaggttca gatagaacgt
     1201 gccgctccac atcaggatca cgccgatggc gagcacgatc cccggcaccg aatagggaag
     1261 cagcgcgagg atctccagaa gcgacttccc cttgaccttc acctttacca ccacgtaggc
     1321 gatcatggtg cccacgaaca tcgcgataat cgcggacgaa atggccagaa agaagctgtt
     1381 cttgatagcg tcctgcgcca tccgcgagga gaagagcacg tgatagtagt tatccaacgt
     1441 catgttggcg aggttgagcg gcagcccgta cgccttcagc cctcccaccg cgaagatgat
     1501 atagagcggc agaatgacga tcacgaacag gctggcgagg cagacgccca gcagcgggaa
     1561 cttggacgtc cggaggttga tcagcatggg tcgcatactc ttgccgcgga taatatcgta
     1621 gtgcccggcc ttcagaatct tccgctgcag ccagagtgcg atgacgacca ccaccacgag
     1681 cagaatggag agcgccgccc cctgccggat tccctcgaag ctcccggccg accggttgat
     1741 cagctcatag atcttggtgg gaagggtgaa tatcccccga gagaagccga gcatcgccgg
     1801 aacgccgaaa tgcgacagcg aggagatcag aatgagcatc gcgccggccg cgacggcggg
     1861 cactacgagc ggcagcgtga tcttccggat cacgtacagc tggttcgccc cggaaatccg
     1921 ggccgattcc tccagcgtcg ggtccatgcg ctcaagcgcg ctgaccacct ggaggaaaac
     1981 gaacgggaag tagtagcaga cctcgacgaa gacgattccg gcgatgctgt tgatgttcat
     2041 aagaggctct ctcgtcccca tcatcagcat ccacagcttg ttgatgtacc ccgaacgcgg
     2101 agagagcagc agcccccacg ccatcgcccc gatgaaggga gggaacatgt acgggatgac
     2161 gaagagcgtc ttcatcagcc ccttgagggg gatgttcgtc cggccgatca gccaggcgta
     2221 aaaaaccccg acgatggtcc cgaccaccgt gacccagaac gcgatcacga tcgtgttcca
     2281 catagcgtcg acattcgagg ggtccatgac gacattcgta aacatactcc agtcgaacgt
     2341 aaaaccatag aaaaacgtgt tgtagacaat catgatcatg ggcatagcca ccgtgatgat
     2401 caaaatcgtc aggcagatca gcatgagaat ctggttcaga tcgagcttgc cctctctgcg
     2461 accgaacatc ttcatgaggc tggcccccct tcgtcttcct ggtagaagcg caaattcctg
     2521 aaagagagcg cgcacggacg cccttcctcc ggcactccgg ccctgaaggc ttcatgggag
     2581 tcctgctgca cgcgaatctc ccgccctccg aggagcacgc ggtattcgaa gagattcccc
     2641 aggtacacca cgtgcttcac gaagccctga accggcccgt cgccgcccac cgtgatatcc
     2701 acagggcggc acgcggcgaa cagcctctcc gcacggagcg cttcgggaat ttcatacgga
     2761 aacgcctgct tcccctccgt accgtcgagg acgtactcct gtccccgccg ttcgaccggg
     2821 atgaagttgg agacgcccaa aaacttgtag atatagctgt ccttggggtt cctgtagaca
     2881 tcctccggta cgcctatctg ccggatgtag ccattccggt ccagaatggc catccggtcg
     2941 gcgatggcca gagccacctc ctgatcgtgg gtgatgtaga tcaccgtgat gcccgtacgc
     3001 cgctgcagct ccttgatctc aaagcgcatc tcctcgcgca ggttcgcgtc caggttggag
     3061 atgggctcgt cgagcaccat cagctgattc gaagaaacca gcgcgcgcgc caaagcgacc
     3121 cgctgctgct gcccccccga gagctgggcg ggcatacgct gttcgtaccc cttcatgcgc
     3181 acctgctcga tcgcgtccag cgctctcttt ttcgcctcat ccttcggaac cttccgcttc
     3241 ttcagggggt agtagacgtt ctcgaac
//
LOCUS       DAOSWW010000200         3266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21231_length_3266_cov_3.883525, whole genome shotgun sequence.
ACCESSION   DAOSWW010000200 DAOSWW010000000
VERSION     DAOSWW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21231_length_3266_cov_3.883525"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PK442_05225"
     CDS             <1..484
                     /locus_tag="PK442_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOO87083.1"
                     /translation="GMAEAASRGDIFLTLTGNTKVIRKEHFEKMKDGVLLGNAGHFDV
                     EISKPDLDSLTESVEETRPGIRTYVLKDGRRLHLLGEGRLVNLACADGHPIEIMDLSF
                     ALQLESAIYMAKNSMKPGLYDVPAEIDRSVMEAKLASMNITLDTMTPEQEAYMKSWVE
                     "
     gene            486..1766
                     /locus_tag="PK442_05230"
     CDS             486..1766
                     /locus_tag="PK442_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO87084.1"
                     /translation="MATLFKNFLAVDGEMAEGRYCSLRVEDGRITAVADKLAALPGDE
                     VVDGERRMAIIPGFVNAHTHAAMSLLRGIGEEAPLMEWLQKKIWPAEANLTREHIYWG
                     TMAALLEMAACGTTAFADMYFEMDAVVEASLEAGMRCAACRGIVGGERGKFEEGLRLA
                     ETWKAHRDMITVQLGPHAPYTVPLQLMQEICAAAAERAMGVHFHFLETEWELSYLRDE
                     LKMTPKEYLESSGILAAPYGILAHCVWLDAETVGAVDFSRMAIAHNPNSNLKLGSGVM
                     DLTGLGGKTKKIALGTDGAASNNRLDIWGEMRCAALLHKGIRRDPMALSACDALRMAT
                     FEGASALGFKKKGLLKEGWVADLVLVDLDQPHYIGIGTENAAPFLVYAGSSADVYATM
                     VNGAWVYRDRVFPGMDSEKILAKAGEMRTDLLGR"
     gene            1896..>3266
                     /gene="alaS"
                     /locus_tag="PK442_05235"
     CDS             1896..>3266
                     /gene="alaS"
                     /locus_tag="PK442_05235"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOO87085.1"
                     /translation="MQWRSGREIRSLFLDFWVSKGSRHVPSFSLIPDDPSLLFTIAGM
                     VPFKPYYLGIQTPESPTAVTSQKCVRTNDIENVGRTARHHTFFEMLGNFAWGDYFKKE
                     AITWAWEFLTEVIGLEPERLSATIYKDDEEAYDVWHNIVGLPDERIFRFDKDANFWFM
                     GTQGPCGPCSEIMYDQGPEYSCGRPECAVGCDCDRYLEIWNLVFTQFDLQKDGSLVPL
                     PKKNIDTGMGLERLTSVVQRVRTDFETDLFRPMIDRACSLGGIQYGYSKKTDLAVRVI
                     ADHIRSVVFMIADGILPSNDGRGYVLRRLLRRAARFGRLLGVDRPFLLDFVPDVIALM
                     GDPYREIVDNRLMVEQIIDVEEKRFGRTLQQGTELLDAEMANLKRSGSAMLSGAVAFE
                     LYDTFGFPLELTSEIVEENGFTVDREGFDREMTAQRERARASSKQKRSEMTGDVYTEL
                     NEALGDT"
BASE COUNT          624 a          898 c         1150 g          594 t
ORIGIN      
        1 cggaatggcg gaggccgctt cgcggggcga tatcttcctg acgctcaccg gcaacacgaa
       61 ggtgatccgc aaggagcact tcgagaagat gaaggacggc gtgctgctgg ggaacgcggg
      121 gcacttcgac gtggaaatca gcaaaccgga tttggacagc ctgacggagt ccgtcgagga
      181 gacgcgcccc ggcatacgga cgtatgtgct gaaggacggc cggagactgc accttctcgg
      241 cgaaggacgt ctggtgaacc ttgcgtgcgc cgacgggcac cctatcgaaa ttatggatct
      301 gagcttcgcg ctgcagcttg aatccgccat ctacatggcg aaaaacagca tgaaaccggg
      361 gctgtacgac gttcctgcgg agatcgaccg gtcggtgatg gaggcgaagc tggcctcgat
      421 gaacatcacg ctcgacacga tgactcccga gcaggaagcg tacatgaaga gctgggtgga
      481 gtagcatggc gaccctcttc aagaacttcc tggccgtcga cggcgagatg gcggaggggc
      541 gctactgctc tctccgcgtc gaggacggca gaataacggc cgtcgcggac aagctggcgg
      601 cgctgcccgg cgacgaggtg gtcgacggcg agcggcggat ggcgatcatt cccgggttcg
      661 tgaacgcgca cactcatgcc gcgatgtcgc tgctgcgggg gatcggcgag gaggcccccc
      721 tgatggagtg gctccagaag aagatctggc ctgccgaggc caatctgacg cgggagcaca
      781 tctattgggg gactatggcg gcgctgcttg aaatggcggc ctgcgggacc actgcgtttg
      841 cggatatgta cttcgagatg gacgcggtgg tggaggcgtc gcttgaggcg ggaatgcgct
      901 gcgcggcttg caggggtatc gtgggcggcg agcgggggaa gtttgaagag gggctgcgtc
      961 ttgcggagac gtggaaagcg catcgcgaca tgatcacggt gcagctgggg ccgcacgcgc
     1021 cgtacaccgt gccgctgcag ctgatgcagg agatctgcgc ggcggcggcg gagcgcgcga
     1081 tgggggtgca cttccacttt ttggagacgg agtgggagct ctcgtacctc cgggacgagc
     1141 tgaagatgac gccgaaggag tacctggagt cctccgggat tctggcggct ccgtacggga
     1201 ttctggcgca ctgcgtctgg ctggatgcgg agactgtggg cgccgttgat ttttccagaa
     1261 tggcgatcgc ccataacccc aacagcaact tgaagctggg gagcggcgtg atggatctca
     1321 ccggcctcgg cgggaagacg aagaagattg ctttaggaac ggacggggcg gcgagcaaca
     1381 accgcctgga catctggggc gagatgcgct gcgccgcgct gctgcacaag ggaatccgcc
     1441 gggatcccat ggcgctttcc gcgtgcgacg cgctccggat ggccacgttc gaaggcgctt
     1501 ccgccctcgg cttcaagaag aagggactgc tcaaggaagg ctgggttgcc gaccttgttc
     1561 tcgtcgacct cgatcagccg cactatatcg gcatcgggac ggagaacgcc gcccccttcc
     1621 ttgtgtacgc cggatcctcg gccgatgtgt acgcgacgat ggtgaacggc gcttgggtct
     1681 accgcgacag ggtattcccc ggtatggatt cggagaaaat cctggcaaag gccggggaga
     1741 tgaggacaga ccttctgggc agataagggg aatctggcac gctgaaggtt gccggggccg
     1801 atcgggcgtt cccggccaag aactcccccg agcggggagg aagatactac cgctcacgga
     1861 aatgattccg ggggctacgg ggaggaagag tgtatatgca gtggagatcg ggtagggaga
     1921 tacgttcttt gtttctggat ttttgggtgt cgaaggggag ccggcatgtt ccaagcttct
     1981 cgctcattcc cgacgatcct tcgctgcttt tcaccatcgc ggggatggtc cccttcaagc
     2041 cgtactatct ggggattcag acgccggagt cgcccacggc ggtgacgtcg cagaagtgcg
     2101 tgcgtacaaa cgacatcgag aacgtcggac ggacggcgcg ccatcatacc ttcttcgaga
     2161 tgctggggaa cttcgcgtgg ggggactact tcaagaagga ggcgatcacc tgggcgtggg
     2221 agttcctgac ggaagtgatc ggcctggagc cggaacgcct ctccgcgacg atctacaagg
     2281 acgacgaaga agcgtacgac gtttggcaca atatcgtcgg tcttcccgac gagcgcatct
     2341 tccgcttcga caaggacgcg aacttctggt tcatggggac gcaggggccg tgcggtccgt
     2401 gttccgagat catgtacgac caggggccgg agtattcgtg cgggcggccg gagtgcgccg
     2461 tggggtgcga ctgcgacagg tacctcgaaa tctggaatct ggtgtttacg cagttcgact
     2521 tgcagaagga cggctctttg gttccgttgc cgaagaagaa tatcgacacc ggtatggggc
     2581 ttgagcgtct gacttcggtt gtgcagcggg tgcgcaccga ttttgagacg gatctcttcc
     2641 ggccgatgat cgaccgggcg tgcagcctgg gcggcattca gtacggctac agcaagaaga
     2701 ccgacctcgc cgtgcgcgtc atcgcggacc acatccgttc agttgtcttc atgatcgcgg
     2761 acggcatcct gccgtcgaac gacgggcggg ggtacgtgct ccgccggctg cttcgacgtg
     2821 cggcgcgctt cggccgtctg ctcggcgtcg accgcccctt cctgctcgac ttcgtccccg
     2881 acgtgatcgc gctgatgggc gatccctacc gtgagatagt ggacaaccgc ctgatggtgg
     2941 agcagatcat cgacgtggag gagaagcgct tcggccgcac gctgcagcag ggaacggagc
     3001 ttctggacgc ggagatggcg aacctcaaac gctccgggag cgcgatgctt tcgggcgccg
     3061 tcgccttcga gctgtacgat acgttcggct tcccgctgga gctgacgtcc gagatcgtcg
     3121 aggaaaacgg cttcaccgtg gaccgcgaag gcttcgaccg ggagatgacg gcccagcgtg
     3181 agcgtgcgcg ggcgtcgagc aagcagaaac gcagcgagat gaccggcgac gtgtacaccg
     3241 aactgaacga ggcgcttggg gatacg
//
LOCUS       DAOSWW010000201         3259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21307_length_3259_cov_3.238764, whole genome shotgun sequence.
ACCESSION   DAOSWW010000201 DAOSWW010000000
VERSION     DAOSWW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21307_length_3259_cov_3.238764"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..559)
                     /locus_tag="PK442_05240"
     CDS             complement(95..559)
                     /locus_tag="PK442_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO87086.1"
                     /translation="MAEQQLVVFALGKEEFGIDISRVREIVRLQNITTIPQSMDFVEG
                     IVNLRGQIVPIVDLCKRFLVADRSGSSDTERRIIVVNMSGQNIGILVDGVSEILRIPD
                     ESIEPTPPIVASGVSSDFIRGVAKVDGRLIIFLDLDRIFSVEEQEALYQSVE"
     gene            complement(682..2727)
                     /locus_tag="PK442_05245"
     CDS             complement(682..2727)
                     /locus_tag="PK442_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO87087.1"
                     /translation="MTIRNKLLVMALVVLAMIGTMAGVTYYRAGSIIEGLVDTAGVEI
                     VKSAAENVDARLNKVEAIVLTAAETVRDAMLRLNVTDEDGVEKIVAALTARTKNEGIM
                     DLYMGHEATGKFSDGTGWKEPDDYDCRTRSWYKEAVAAGKGKIIFTEPYVDGITKQVV
                     ISTATAIYDDAGKLLGVVGGDMDISGLSDYVVGLQIFGKGSGLMLLKSGIFVAYNEKD
                     DVLKANMMTDEKFAEPIRNIARKMVSGETGYESYVYQGEERQMFFAPTQRAFYLGILF
                     PAAEINAMVNALTFILLGLAVVAVLVTGGTIFFIARGLTKSIRSMESVTEKLGEGDLT
                     VSYEASGKDEIAHISGILNGMVFSLREVMGSIRNESQETAARAETLASLSEETLASME
                     EVSSSIEKVQDMMEHSSSALQETNASIEEMAAGAQTAARATNEGADGATNASDAANAS
                     LQEVGRAINNIRNAGSESAKSIDRIKELGKSVEAISGFVTTITSIADQTNLLALNAAI
                     EAARAGEAGRGFAVVAEEVRKLAEESARAAHEVSKLINELQKHSDDSIAATEKTGTIL
                     DDTMKGAEEMRQKLKGAVDAIVKIAEAVQNIAAVSEEQAASSEEMTSAIQSVTESTGQ
                     VVSSVGSIRGASHETTKAAESIATEAQAMAVTSEKLQTLVSRFIVDDARRSGLALKK"
BASE COUNT          584 a         1062 c          863 g          750 t
ORIGIN      
        1 ggcgagattt tcggccggta gggagacgtt cccaaaaaaa gcgaataaaa aagaccccgt
       61 atcgctacgg ggtccgccat gcattgggtt cgcgctactc aaccgactgg tagagcgctt
      121 cctgctcctc gaccgagaag atgcggtcga gatcaaggaa gatgatcagc ctgccatcga
      181 ccttggccac accccggatg aagtccgaac tgacgccgct ggccacgatg ggaggcgtcg
      241 gctcgatcga ctcgtccggg atgcgaagta tctcggagac tccgtccacg aggatgccga
      301 tgttctgtcc cgacatgttg acgacgatga tccggcgttc cgtatcgctg gagccgcttc
      361 tgtccgcgac cagaaacctc ttgcacagat cgacgatcgg gacgatctgc ccccgaagat
      421 tgactatccc ctcgacgaag tccatggact gggggatggt ggtgatgttc tgcagtctga
      481 cgatctcccg gactcgggag atgtcgatgc cgaattcctc cttgccgaga gcgaacacga
      541 caagctgttg ttccgccatt ttttttcctc cttccgcaag atcctgctga ataaactctt
      601 caggacaatt ctaacagata ttgtgatgaa gagcacccga atcccgcaaa atcacacaat
      661 ggattccggc gcacacccgc gtcacttttt gagcgcgagt ccgctccgcc tcgcatcgtc
      721 cacgataaat cgcgagacaa gcgtttgcag cttctcggac gttaccgcca tcgcctgcgc
      781 ctcggtcgcg atcgattcgg cggccttcgt cgtttcgtgc gacgctcccc ggatagagcc
      841 gaccgagctc acaacctgtc cggtggattc ggtgacgctc tgaatcgccg aggtcatctc
      901 ctcgctcgac gcggcctgct cctccgagac ggcggcgatg ttctgcaccg cttccgcgat
      961 cttgacgatt gcgtccacgg cgcccttgag cttctgccgc atctcttccg cgcccttcat
     1021 cgtatcgtcc agtatagttc ccgtcttttc cgtggctgcg atggagtcgt ccgaatgctt
     1081 ctgaagctcg ttgatgagct tgcttacctc gtgcgccgcc ctggccgact cctcggcgag
     1141 cttgcggacc tcttcggcca ccaccgcgaa gcctcttccg gcctcgccgg ctctggccgc
     1201 ttctatcgcc gcgttcagcg ccagcaggtt cgtctggtcc gctatcgacg tgatggtcgt
     1261 cacgaatccg gagatagcct ccaccgactt gccgagctcc ttgatccggt cgatgctctt
     1321 cgcggactcg ctccccgcgt ttcgtatgtt gttgattgcg cgtcctacct cctgcagcga
     1381 agcgttcgcg gcgtcggagg cgttcgtcgc gccgtccgct ccttcgttgg tcgcccttgc
     1441 cgcggtctgc gcgcccgccg ccatctcctc tatcgacgca ttcgtctcct gcagcgcgga
     1501 ggagctgtgc tccatcatgt cctggacttt ttcaatggag ctggagacct cttccatgga
     1561 cgcaagcgtc tcctcggaga gggaagcgag cgtctcggcg cgcgcggccg tctcctgcga
     1621 ctcgtttcgg atcgatccca tgacttcgcg cagcgagaag accatgccgt tcagaatgcc
     1681 ggaaatatgc gcgatctcgt ccttgcccga ggcctcgtac gagaccgtca ggtcgccctc
     1741 tcccagcttc tccgtcacac tctccatcga gcgtatcgac ttcgtcaggc cgcgcgcgat
     1801 gaagaagatc gtccctcccg tcaccaggac ggcgacgacc gcgagaccga gcagaataaa
     1861 ggtgagcgcg ttcaccatcg cgttgatctc cgcggcgggg aagaggatgc cgaggtagaa
     1921 agcgcgctgc gtaggggcga agaacatctg ccgctcctcc ccctggtaga cgtagctctc
     1981 atatcccgtc tcgccggaga ccatcttccg cgcgatattc cgtatcggct cggcgaactt
     2041 ctcgtcggtc atcatgttcg ccttcaaaac gtcgtctttc tcattgtagg caacgaagat
     2101 gccgcttttc aggagcatca ggccgcttcc cttgccgaaa atctggagcc cgacgacgta
     2161 gtcgctcaga ccgctgatgt ccatgtcgcc cccgacgact cccaggagtt ttccggcgtc
     2221 atcgtagata gccgtggcgg tggagatgac gacctgcttc gtgatcccgt cgacgtaagg
     2281 ctcggtgaag atgatctttc cctttcccgc cgccaccgct tccttatacc acgagcgggt
     2341 tctgcagtca tagtcgtcgg gctccttcca gccggtgccg tcggagaact ttcccgtcgc
     2401 ctcgtgcccc atgtagagat ccatgatccc ctcgtttttc gtccgggcag tcaaggcggc
     2461 gacgatcttt tccacgccgt cctcatccgt cacgttcagc cggagcatcg cgtcgcggac
     2521 cgtctccgcg gctgtaagca cgatcgcctc gaccttgttc agacgcgcgt cgacattctc
     2581 ggcggcgctc ttcacgatct ccacgccggc cgtgtccacc aggccctcga taatactccc
     2641 ggcccggtag tacgtcactc ccgccatcgt ccctatcatg gcgagcacca caagcgccat
     2701 caccagcagt ttgttcctga tcgtcaattt ttttccccct cttccgtgat attatcggaa
     2761 cttcctctcc gcgcacatga agcctaaagc agcgagcgga gcatttcccc ccggcacgtt
     2821 cccctgaaac gcttctcagc aatgaagaaa agaatttcct cctttcagaa aagcttctac
     2881 tttgttcaat tcaacacaag gattgctttc attcgtcttt tgaaaggcag tcaaacaggc
     2941 ttttttttgt atcggccctt tctttccgcc aaagaagttt ctgaagtttt aatctataga
     3001 acgggagctt gcgcagccgt ttccggcggt cattcctgac ccgccccgag tcctcgaaga
     3061 ggcttcgctt atcgccgaaa tcgcgccgcg gagaaccgtc ataaaaaagg ggggagaagg
     3121 catacgtttg cctcctcccc cctcgtttct acgttgtgaa agcccaggtc actttttcag
     3181 cgcgagtccg gttccttccg ccgttgtctt gaagcgggct acggagcgct ggagctcctc
     3241 cgagagatcg acaaggtct
//
LOCUS       DAOSWW010000202         3259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21312_length_3259_cov_2.600811, whole genome shotgun sequence.
ACCESSION   DAOSWW010000202 DAOSWW010000000
VERSION     DAOSWW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21312_length_3259_cov_2.600811"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK442_05250"
     CDS             complement(<1..387)
                     /locus_tag="PK442_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87088.1"
                     /translation="MFELHRRIREFAARIQWEMKGRPIPLPNALKQVILLEHAQMHGI
                     RVFVETGTYEGKTLAAMVPYFDRLYSVELGEALYERACARFASVPKVRLFQGDSGKRL
                     EDILALERERRCLFWLDAHYSRGDTAR"
     gene            complement(401..1417)
                     /locus_tag="PK442_05255"
     CDS             complement(401..1417)
                     /locus_tag="PK442_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87089.1"
                     /translation="MKYKRLVRLILCSSLLVFAGQFLLFRASGVYDLALKSYLYDNAS
                     WVKEPPRVLLMGSSRCFHQLVPSVIAEQNGLKITDVVNAGQVAAGPFEMLHTYTQHID
                     VFGNVEFIFYVLDADFFFESLHIDKPYEKILLSRRQWEIIADEHGNFYHLPVVLFANA
                     VRGSPRKTGAEMGFEGLPHMDFLNIVMEDRSARYKKDLEHFPLSDFQLKSMVQLKEAF
                     EKNGGTLVFVLTPFWDIRLLGDDFFERTRIAARALDETLGPVRLIGSWKRDAYGLGYT
                     DFLDNSHLSLSGAKKLTASLFKNIASIRSASPIFLEERLFGKAGEYEETTGTRFHGHI
                     HEQR"
     gene            complement(1432..2871)
                     /locus_tag="PK442_05260"
     CDS             complement(1432..2871)
                     /locus_tag="PK442_05260"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO87090.1"
                     /translation="MSIWRRSMLFNSYVFLFLFLPATWFSFRLACSRRATNAALAVLL
                     VASLLFYSYWNPPFILLILFSILFNYAWGRGIERAVLQDRNVKAWLFSGLGVNLALIA
                     YFKYANFFMANLALLCGREWLHRDIFLPLGISFFTFQQMAYLVDCSKGLVGGHSFTHY
                     ALFVSFFPQLIAGPIVRYDQIIPQFSRLRTFALSYRNIAIGLTLLSLGLFKKLVVADT
                     LAPWAAAVFDTTGVPPVLETWVGVLCYTFQIYFDFSGYSDMALGLGRLFNIELPVNFA
                     SPYKACSMVEFWRRWHISLSTFLRDYLYIPLGGNRKGTLRRYGNLMTTMLLGGLWHGA
                     SWNFVIWGGLHGLYLCANHSLAAVAPMRAFPRLQRFLGWLVTFFSVVVAWVFFRAHTL
                     GRALELFSGMFGLNGGCALPGSPGTDRLVVLGACFLLVVAAPNSQEWTFTRFREGTFT
                     ARWAVAVGGVLLISVCFLNRVSEFLYFQF"
     gene            2987..>3259
                     /locus_tag="PK442_05265"
     CDS             2987..>3259
                     /locus_tag="PK442_05265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO87091.1"
                     /translation="MIGFHRKKQERYRRFHERGKAAVLLKILQILPELHEGGVERHVL
                     WLSNGLAAGGHEVVVVSAGGKMERELRGVEHVRLPVHRKNPATAAWA"
BASE COUNT          736 a          916 c         1004 g          603 t
ORIGIN      
        1 tctcgccgtg tcaccgcggg agtagtgcgc gtcgagccag aagaggcagc gtctctccct
       61 ctcaagcgca aggatgtcct caagtctctt gccgctgtcc ccctggaaga ggcgcacctt
      121 gggtacggag gcgaaacgag cgcaggcccg ttcatacagc gcttctccca gctcgaccga
      181 gtagaggcgg tcgaaatacg ggaccatggc ggcgagcgtt ttcccctcgt aggtccccgt
      241 ctcaacgaac acgcggatgc cgtgcatttg ggcgtgctcc agaagtatca cctgtttgag
      301 ggcgttcggc aggggaatcg gcctgccctt catttcccat tgaatacgtg ccgcgaactc
      361 gcggatgcgc cgatgcaatt caaacatacc gaaagtctcc ttatctttgc tcatggatgt
      421 gcccgtgaaa tctcgtgcct gttgtctctt cgtattcccc ggcctttccg aagagacgct
      481 cctccaaaaa gatcggcgaa gccgagcgga tcgatgcgat gttcttgaaa agcgaggcgg
      541 tcagcttctt cgcgccggag agcgacaggt gcgaattgtc caggaagtcc gtatacccca
      601 gcccgtaggc gtcccgcttc cagcttccta tgagcctgac gggtccaagc gtttcgtcga
      661 gagcacgggc tgcgatgcgc gtccgttcga aaaaatcgtc gccgagcaga cgtatgtccc
      721 aaaaaggcgt gagcacgaac accagggttc cgccgttctt ttcgaaggct tcctttaact
      781 gtaccatact tttcagctgg aaatcggaga ggggaaagtg ctccaggtcc tttttatacc
      841 gggcgctcct gtcctccatg acgatgttca gaaagtccat gtgcggcagc ccctcgaacc
      901 ccatctccgc ccccgttttg cgcggcgatc ccctgaccgc gttcgcgaag aggacaacgg
      961 gaaggtggta gaagttgccg tgttcgtccg cgatgatctc ccactgcctt ctgctcagca
     1021 gaatcttttc gtagggcttg tcgatgtgca aggactcgaa gaaaaagtcg gcgtcgagga
     1081 cgtagaagat gaactccacg ttgccgaata cgtcgatgtg ttgtgtgtag gtgtgcagca
     1141 tctcgaacgg ccccgccgcg acctgccccg cgttgacgac gtccgttatt ttcagcccgt
     1201 tctgctccgc gatgacggag gggacgagct ggtggaaaca gcggctgctt cccatcagca
     1261 gcacgcgcgg gggttccttt acccatgaag cgttgtcgta caggtatgat ttcagggcca
     1321 ggtcgtagac gccggaagcg cgaaagagca ggaactgtcc ggcgaagacc aggagagagg
     1381 agcagagaat aagacggacc agacgtttgt atttcatggg aaccctctct tctagaattg
     1441 gaaatagaga aactcggaga cccggttcag gaaacagacg ctaatgagca gcacgcctcc
     1501 gaccgcgacg gcccagcggg cggtgaaggt cccctcccgg aagcgtgtga aggtccattc
     1561 ctggctgttc ggcgccgcaa caaccagtaa aaaacaggcc ccgaggacga cgagccggtc
     1621 ggtccccggc gatcccggga gcgcacaccc tccgttcagg ccgaacatgc cgctgaagag
     1681 ctccaacgct ctcccgagcg tgtgcgcccg gaagaagacc catgcgacga caacggaaaa
     1741 aaaggtgacg agccatccga gaaagcgttg cagacgcgga aacgctctca tcggcgccac
     1801 tgccgcgagc gagtggttcg cgcagaggta cagaccgtgc agccctcccc aaatgacgaa
     1861 gttccacgaa gcgccgtgcc agagcccccc gagcagcatc gtcgtcatca ggttgccgta
     1921 gcgccggagc gttcccttcc ggttcccccc cagcgggata tacaggtagt ccctcagaaa
     1981 ggtcgagagc gagatgtgcc atctgcgcca gaattccacc atcgagcacg ccttgtaggg
     2041 agaggcgaag ttgacgggta gttcgatatt gaacagtcgt ccgagcccga gcgccatatc
     2101 cgagtagccc gagaagtcga agtagatctg gaacgtgtag cagagcaccc cgacccaggt
     2161 ctcaaggacc gggggaacgc cggtcgtgtc gaagacggcc gccgcccacg gcgcaagcgt
     2221 gtccgcgacg accagcttct tgaagagccc gagcgagagt agcgtgagtc ctatcgcgat
     2281 gttccggtag ctcagcgcaa acgtgcggag ccgggagaac tgcggaatga tctgatcgta
     2341 gcggacgatc ggcccggcga tgagctgcgg gaagaaggag acgaaaaggg cgtagtgcgt
     2401 gaaggagtgt cctccaacca gccccttcga gcagtcgaca aggtaggcca tctgctgaaa
     2461 ggtgaaaaag gagatgccaa ggggaagaaa gatatcgcgg tggagccact cgcggccgca
     2521 gaggagcgcc aggttggcca tgaagaagtt cgcgtacttg aaataggcga tcagcgccag
     2581 gttgacgccc aagccgctga agagccatgc ctttacattc ctgtcctgta aaacggcccg
     2641 ttcaatgccg cgtccccagg cgtaattgaa gaggatcgaa aagagaatga ggaggatgaa
     2701 cggagggttc cagtacgaat aaaaaagcag cgacgcaaca agaagcacgg ccagggcggc
     2761 gttcgtcgct ctcctggagc atgcgaggcg gaaactgaac caggtcgcgg gcaggaagag
     2821 aaacaggaaa acataggaat taaagagcat gcttcttctc catatcgaca tgcggcccgt
     2881 tcttccgatt ctgttctgga gagccgcatc ggatgttgcg tattatatcc gatactttcc
     2941 gatttgtaat tcggcgctcc ggtcttttgg acaaggagag ggaaatatga taggcttcca
     3001 tcggaaaaaa caggagcgct accggcgctt ccatgagcgt ggaaaggcgg cggtcctctt
     3061 gaagattctc cagattcttc ccgaactgca tgaaggcggc gtcgagcggc acgtgctgtg
     3121 gctctccaac ggccttgcag ccggggggca tgaggttgtc gttgtctcgg ccggtggaaa
     3181 gatggagagg gagctgcggg gcgtagagca tgtccgcctt ccggtgcaca ggaaaaaccc
     3241 ggcgacggcc gcgtgggcg
//
LOCUS       DAOSWW010000203         3255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21347_length_3255_cov_3.086875, whole genome shotgun sequence.
ACCESSION   DAOSWW010000203 DAOSWW010000000
VERSION     DAOSWW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21347_length_3255_cov_3.086875"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PK442_05270"
     CDS             complement(<1..609)
                     /locus_tag="PK442_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOO87092.1"
                     /translation="MKNERDFPQSTAGEERTVPTVPALVDTHCHVHADDYGAETAAVV
                     ERAAQAGVARMLVIGSDIRTSRLAVEAAEQFASSGVFAAVGIHPHEAATEPSCSVEGK
                     GIPEELRGLAAHPRVAAIGETGLDYHYDHSPRDQQRNSFREHIRFSAEAGKPLVIHGR
                     ESYDDLRSILHEERGERIRAVVHCFSGTEDDARDFLEMGYFLS"
     gene            complement(766..2745)
                     /gene="metG"
                     /locus_tag="PK442_05275"
     CDS             complement(766..2745)
                     /gene="metG"
                     /locus_tag="PK442_05275"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOO87093.1"
                     /translation="MKEQKFAGGIANMNEKKSFYLTTPIYYVNDIPHIGHAYTTIAAD
                     AMCRYKRMKGFDVFFLTGTDEHGQKIQQSAAAKGLTPRELADRTVRNFQDLWKALNIS
                     NDDFIRTTEERHYKTVQSIFKKLLDQGDIYKGKYEGWYCVPCETYVPESSMGEDKTCP
                     DCKRPLQMMEEESYFFRASKYVDQLLAYYEKNLKGVMPRSRYNEIISFLKGGVRDQSI
                     SRTTLKWGIPVPGDDAHVVYVWFDALINYVTACGYLDDRKKFDTFWPEVRHLVGKDII
                     RFHCVIWPIMLLALGVNPPVSVFAHGWWTVEGEKMSKSKGNVVDPFEMVERYGADPFR
                     YFLLREVPFGLDGDFSESAVVGRINSDLANDLGNLLNRTLQMIDNFFQGSLPASCRRG
                     EREEEIVQRAAAVLAEVDEKIEDFAFDDALKSIWSFISRGNKYIDETMPWKLAKEGKT
                     EELSSVLNTLYDILRLSAMLVAPFIPETAARIWEQLGLDGEPVNMRFDGFSWGDRAPG
                     HTVKKGKVLFPRIDLEEWKKAREAKAPAPAQPASEEPAIVHEELVGIDSFKAVELRVA
                     QILNVEEIPKSKKLYKLTVDLGYEKRTIVSGIKEFFTPDELLHKKIVVVVNLQPAKLC
                     GVESNGMLLAAGDGKKTTLALLTPDKDIPLGSRVS"
     gene            complement(2832..>3255)
                     /locus_tag="PK442_05280"
     CDS             complement(2832..>3255)
                     /locus_tag="PK442_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA (cytidine-2'-O-)-methyltransferase"
                     /protein_id="HOO87094.1"
                     /translation="YGFLPDKQGERERAILSVASVPWTLLFYVSPHKAEKHLLHLCAL
                     LGDRRAALVREISKIYEEARRGVLSALAESVRGGVKGELVLVVEGSSGERGPSAELPE
                     WRERAVALASEGVSSKEVVKIVSEEYSVPKNEVKSFLF"
BASE COUNT          559 a         1034 c          985 g          677 t
ORIGIN      
        1 ggagaggaag taccccatct ccagaaaatc ccgcgcatcg tcttccgtcc cggagaagca
       61 gtggacgacg gcgcgtatcc gctccccgcg ctcctcatgg agaatgctgc gcaggtcgtc
      121 gtaggactcc cgcccgtgga tgacaagcgg cttgcccgcc tccgcggaga agcgaatatg
      181 ctcgcggaag ctgttccgct gctggtcgcg cggcgaatgg tcgtaatggt agtcgagtcc
      241 tgtctcgccg atcgccgcaa cgcgggggtg ggcggccaat cctcgaagct cctccgggat
      301 cccctttccc tccacggaac aggagggttc cgtcgccgcc tcgtgcgggt gaatccccac
      361 ggcggcgaac accccggagg aagcgaactg ttccgcggcc tccacggcaa ggcggctcgt
      421 ccgtatgtcg cttccgatga cgagcatgcg cgccaccccg gcctgcgcgg cgcgctccac
      481 taccgcggcg gtttccgcgc cgtagtcgtc cgcgtgcacg tggcagtgcg tatccacgag
      541 cgccggaacg gtgggtacag tacgctcttc gcccgcggta ctctgcggga aatctctctc
      601 atttttcatc tgcttcatcc tcattttctg ttcgttatca cgctgttccg cagtgttcgg
      661 cggatacagg aatcagggag cctccccgga tttccggaga ggctccccgt cactgatacc
      721 tgccgggact ccccgctgcg ggagcggcgt cccggatgcg ttctcttagc tcacgcggct
      781 gccgagaggg atatccttgt cgggggtaag cagcgcgagc gtggttttct ttccgtcccc
      841 ggcggcgagc agcatgccgt tgctctccac gccgcagagc ttcgcgggct ggagattcac
      901 caccacaacg attttcttgt gcagcagctc gtccggcgtg aagaactcct tgatgccgga
      961 gacgatggtc cgcttctcat atccgaggtc gacggtcagc ttgtagagct tcttcgactt
     1021 cggtatctcc tccacgttca gaatctgcgc cacgcgcagc tcgacggcct tgaagctgtc
     1081 gatcccaacc agctcctcgt ggacgatcgc gggctcctcc gaagcaggct gcgcgggggc
     1141 cggcgctttc gcctcccgcg ccttcttcca ttcttcaagg tcgatgcgag ggaagaggac
     1201 ctttcccttc ttcaccgtgt ggccgggcgc ccggtcgccc cacgagaatc cgtcgaagcg
     1261 catgttcacc ggttcgccgt caagtccgag ctgctcccag atccgcgccg cagtttccgg
     1321 aatgaagggg gccacgagca tggcgctcag acgaaggatg tcgtagagtg tgttgagcac
     1381 ggaggagagc tcttcggtct tcccctcctt ggcgagcttc cacggcatgg tctcgtcgat
     1441 atacttgttc ccgcgcgaga tgaagctcca gatgctcttc agcgcgtcgt cgaaggcgaa
     1501 gtcctcgatt ttttcgtcga cctcggcgag gacggcggcg gcccgctgta cgatctcctc
     1561 ctcgcgctcc cccctcctgc atgatgcggg aagcgatccc tggaagaagt tgtcgatcat
     1621 ctggagagtt ctgttgagca gattccccag gtcgttcgca agatcggagt tgatccgccc
     1681 cactaccgcg ctctcggaga agtcgccgtc gaggccgaac ggaacctcgc ggagcaggaa
     1741 gtagcggaag gggtccgcgc cgtagcgctc caccatctcg aaggggtcca cgacgttccc
     1801 cttcgatttc gacatcttct ccccctcgac ggtccaccag ccgtgggcga acacggaaac
     1861 gggagggttg acgccgagcg cgaggagcat aatgggccag atcacgcagt ggaagcggat
     1921 gatgtccttg ccgacgagat ggcgcacttc cggccagaag gtgtcgaact tcttccggtc
     1981 gtcgaggtag ccgcaggccg tcacgtagtt gatcagcgcg tcgaaccaga cgtacaccac
     2041 gtgcgcgtcg tcgccgggca ccgggatccc ccacttcagg gtggtccggg agatggactg
     2101 gtcgcgcacg ccccccttga ggaagctgat gatctcgttg tagcggctgc gcggcataac
     2161 gcccttcaaa ttcttttcgt aataggccag gagctggtcg acgtacttcg acgcgcggaa
     2221 gaagtagctc tcctcctcca tcatctggag agggcgtttg cagtcgggac aggttttgtc
     2281 ctcgcccatg ctgctctcgg gaacgtacgt ctcgcagggg acgcagtacc acccctcgta
     2341 cttcccttta tagatgtcgc cctgatcgag gagcttcttg aagatggact gcaccgtctt
     2401 gtagtgacgc tcctcggtgg tgcggatgaa gtcgtcgttg gagatgttga gcgccttcca
     2461 gagatcctgg aagttgcgca ccgtcctgtc ggcaagttcg cgcggcgtga gccccttggc
     2521 tgcggcgctc tgctgaatct tctgtccgtg ctcgtcggtc ccggtgagga agaaaacgtc
     2581 gaaccccttc atccttttgt acctgcacat cgcgtcggcg gcgatggtcg tgtaggcatg
     2641 cccgatgtgc gggatgtcgt tgacgtagta gatcggggtg gtgaggtaga agctcttctt
     2701 ttcgttcatg tttgcgatac ctcctgcaaa tttctgctcc ttcacgagaa cggactgagc
     2761 gcggaacggt tcacgccgtc cggctgaata accgcccgcg atgcggaacg ttattgattt
     2821 ccgccggact cctagaaaag gaacgatttg acttcgtttt tcggtacaga atactcctcc
     2881 gagacaattt tgacaacctc tttagaggag actccctccg acgcaagcgc tacggcgcgc
     2941 tctctccact ccggcagttc cgctgaaggc ccgcgctccc cggacgatcc ctcgacaacg
     3001 aggacgagct ctcccttcac gccgccgcgc accgactccg caagcgccga aagaacgccg
     3061 cggcgcgcct cctcgtagat cttgctgatc tcgcggacaa gcgccgccct gcggtccccc
     3121 agaagtgcgc agaggtgcag aaggtgtttc tccgccttgt gcggcgagac gtaaaagagg
     3181 agcgtccacg gtacggaggc cacggagagg atcgcccgct cgcgttcccc ctgtttgtcc
     3241 ggaagaaaac cgtag
//
LOCUS       DAOSWW010000204         3254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21359_length_3254_cov_3.811816, whole genome shotgun sequence.
ACCESSION   DAOSWW010000204 DAOSWW010000000
VERSION     DAOSWW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21359_length_3254_cov_3.811816"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1847
                     /locus_tag="PK442_05285"
     CDS             <1..1847
                     /locus_tag="PK442_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO87095.1"
                     /translation="KLDLEVLPAYMSAPEAMTDDAIEIHPGTPNVYFTQKIRKGEETA
                     PIFSRPDVITVEDDFYVGRQPHMPVEPDVGFAYTDHEGRLVIHSKSIGVHLHLAMIAP
                     GLGVEPEKLVLVTNPAGGTFGYKFSPTMEALVGAAALATGHPVALKYTWKQQQQYTGK
                     RSPFFVNMKFAADKEGKLLAMETDFSVDHGPYSEFGDLLTLRGIQFMGAGYDIPNIRG
                     EGRTVCTNHAWGSAFRAYGSPQSLFSSEVLMDELAEKLGMDPLEIRYKNAYRPGSTNP
                     TGQAPESYSLPKMLEALRPKYELAKKRAAEGSTTRFKKGVGLSVGVYGCGLDGPDGSE
                     ARLDMNPDGTITVCTAWEDHGQGADAGAIGTAHEALRPLGISPDKLKFTWPNTAKCPN
                     SGPAGGSRSQVMTGNAIRVACETLLRETAKPKTGFLKRDGGFMTYNELVAAGKPTSFT
                     GKWSAVEGTACNEDGQGKPFVIYMYGVFMAEITVDTETGKTSVDRMTLMCDCGKINNR
                     LVVDGQNWGGMAQGIGLALTEDFEDIEKHSTMPGAGFPYIKDIPDDMEIIYFEEHRED
                     GPHGAAGIGELPLSSPHASIINGIYNATGVRITRLPAYPEKVLAGLKK"
     gene            1905..>3254
                     /locus_tag="PK442_05290"
     CDS             1905..>3254
                     /locus_tag="PK442_05290"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO87096.1"
                     /translation="MEKARLLEFESKCVQEEPPYCQAACPLHLDGRALCAFVREGRID
                     DARKLIERTLPLPEALARICDAPCREACLRRELGGAIELGALERACVEQGPLRRAPLL
                     IPKNGKRVAVVGGGLSALCAASEGIRKGYSVSVFCAEDRAAALTALSPLITDEIARRE
                     LAWLEKGGVLFLPLEDLPPLSELSVRFDAAYAGLDDPFAACATAEATASVNPVSLETS
                     VRGLFAGGAAPSFILRAADGRRGMKSAERFLQGASLFSSREKEGPYATRLFTSTEGMA
                     SAEPEAVPQEGYSPEEAIREAKRCILCECMECVKHCAYMQHYKGYPKKFAREIYNNLS
                     VIQGTRLANGMINSCTLCGRCERICPNGFSMADLCREARREMLRQEKMPPSAHDFALE
                     DMRFNNSPRATLLRHEPGKETSAYLFLPGCRLAGCTPEQTTALYAFLRDRLEGGVGFW
                     "
BASE COUNT          624 a         1093 c         1013 g          524 t
ORIGIN      
        1 tgaagctcga cctcgaagtc ctgccggcgt acatgagcgc gcccgaggcg atgaccgacg
       61 acgccatcga gatccacccc ggaacgccga acgtctactt cacgcagaag atcaggaagg
      121 gcgaagagac tgctcctatc ttctcacgcc ccgacgttat cacggtcgag gacgatttct
      181 acgtcggccg tcagccgcac atgcccgtcg agcccgacgt cggcttcgcg tacaccgatc
      241 acgagggacg ccttgtcatc cactcaaagt ccatcggcgt ccacctgcac ctcgccatga
      301 tcgcccccgg actcggcgtc gagcccgaga agctcgttct tgtgacaaac cccgccggag
      361 gcaccttcgg ctacaagttc agcccgacca tggaggcgct cgtcggcgct gcggccctcg
      421 ccacgggaca cccggtcgcg ctgaagtaca cgtggaaaca gcagcagcag tacaccggca
      481 aacgctcccc cttcttcgtc aacatgaagt tcgccgccga taaagagggc aaactgctcg
      541 ccatggagac ggatttctcg gtcgatcacg gcccctactc cgagttcggc gacctcctca
      601 cgctccgcgg tatccagttc atgggcgccg ggtacgacat cccgaacatc cggggcgagg
      661 gacggacagt ctgcacgaac cacgcgtggg gctccgcctt ccgcgcgtac ggatcgccgc
      721 agtcgctctt ctcctccgag gtgctgatgg acgagctggc ggagaagctg ggaatggacc
      781 cgctggagat ccgctacaag aacgcctacc gccccggaag caccaatccg accggccagg
      841 cgcccgagtc ctactcgctg ccgaagatgc tcgaagcgct ccgcccgaag tacgaactgg
      901 cgaagaagcg cgccgcggag ggctccacaa cacgcttcaa gaagggcgtc ggcctttcgg
      961 tcggcgtgta cggctgcggc ctcgacgggc ctgacggctc cgaagcgcgg ctcgatatga
     1021 acccggacgg gacgatcacg gtctgcaccg cgtgggaaga ccacggacag ggcgccgacg
     1081 ccggcgcgat cggcacggcg cacgaggcgc tgcgtcccct gggaatctcc cccgacaagc
     1141 tgaaattcac ctggccgaat acggcgaagt gccccaattc cggccccgcg ggcggctccc
     1201 gttcccaggt catgacgggg aacgccattc gcgtcgcctg cgagacgctg ctgcgggaga
     1261 ccgcgaaacc gaagacaggc ttcctgaaac gggacggcgg ctttatgacc tacaacgaac
     1321 tggtcgccgc cggaaagccg acctccttca ccggcaagtg gtccgccgtc gagggaacgg
     1381 cctgcaacga ggacggacag ggcaagccgt tcgtgatcta catgtacggc gtcttcatgg
     1441 cggagatcac cgtggacacg gaaaccggaa agacctcggt cgacaggatg acgctgatgt
     1501 gcgactgcgg aaagatcaac aaccgcctcg tcgtcgacgg tcagaactgg ggcggcatgg
     1561 cccagggcat cggcctcgcg ctcaccgagg acttcgagga tatcgagaaa cactccacca
     1621 tgccgggggc gggattcccg tacatcaagg acatccccga cgatatggag atcatctact
     1681 tcgaggaaca tcgtgaagac gggccgcacg gcgcggcagg catcggcgag cttccgctct
     1741 ccagcccgca cgcatccatc atcaacggca tctacaacgc gacgggcgtt cgtatcacca
     1801 ggctgcccgc atacccggag aaggtactgg ccgggctgaa aaagtaacag tatccgggga
     1861 ggcgttcctc gcggggcgcc tccctccgtt ccgggaggtg cgccatggaa aaagcacggc
     1921 tgctggaatt cgaatcgaag tgcgttcagg aggagcctcc gtactgccag gcggcttgtc
     1981 cgctgcatct cgacggacgg gccctctgtg cttttgtccg cgaaggccgg atcgacgacg
     2041 cccggaagct gatcgagagg acgctccccc tgcccgaagc gcttgcgcgt atctgcgacg
     2101 cgccgtgcag ggaagcctgc ctccgccgcg aactcggcgg cgcgatcgag ttgggcgctc
     2161 ttgaaagagc ctgcgtcgaa caggggcctc tccggcgcgc tccgctcctt attccgaaga
     2221 acggaaagag ggtcgccgtc gtcggaggcg gactgagcgc gctctgcgca gcctcggaag
     2281 gcattcgcaa agggtactcg gtctccgtct tctgcgcgga ggaccgggcc gcggcgttga
     2341 ctgccctctc ccccctgatc accgatgaga tcgcgcgccg cgagcttgcc tggctcgaaa
     2401 aaggcggagt cctctttctg ccccttgagg acctccctcc gctttcagag ctttccgtac
     2461 gtttcgacgc cgcctacgcc ggtctggacg acccgttcgc ggcctgcgcg actgcggaag
     2521 cgacggcttc agtgaacccc gtgagccttg agacttcggt cagaggactt ttcgccggag
     2581 gcgccgcccc ctccttcatc cttcgagccg ccgacggacg gcggggaatg aagtcggcgg
     2641 agcgttttct ccagggcgcg tccctcttct cctcacggga gaaggagggg ccgtacgcca
     2701 cccgtctgtt cacctccacc gaggggatgg cttcagcgga gccggaagcg gttccgcagg
     2761 aaggctactc ccccgaagag gccatccgcg aggcgaaacg ctgcatcctc tgcgagtgca
     2821 tggagtgcgt gaaacactgc gcctacatgc agcactacaa agggtatccc aagaagttcg
     2881 cgcgggagat ctacaacaac ctctccgtga ttcagggaac gaggctggcg aacggcatga
     2941 tcaactcgtg caccctctgc ggccgctgcg aacgcatctg ccccaacggc ttctccatgg
     3001 ccgatctctg ccgcgaagca aggcgggaga tgctccgcca ggagaagatg cccccttcgg
     3061 cgcacgactt cgcgctggag gacatgcgct tcaacaactc tccaagggcg acgctgttgc
     3121 ggcacgagcc tggaaaggag acgagcgcct acctcttcct cccgggatgc cggctcgcgg
     3181 gctgcactcc cgaacagacg accgccctct acgccttttt gagggaccgt ctggagggcg
     3241 gcgtcggttt ctgg
//
LOCUS       DAOSWW010000205         3248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21421_length_3248_cov_4.502975, whole genome shotgun sequence.
ACCESSION   DAOSWW010000205 DAOSWW010000000
VERSION     DAOSWW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21421_length_3248_cov_4.502975"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PK442_05295"
     CDS             complement(<1..206)
                     /locus_tag="PK442_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HOO87097.1"
                     /translation="MHISPTIFREYDIRGIADVDLTDQVVSAIGQAFGTWLVRQGVRK
                     IVVGGDVRVSTRRISGALVEGLLRT"
     gene            357..1283
                     /locus_tag="PK442_05300"
     CDS             357..1283
                     /locus_tag="PK442_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOO87098.1"
                     /translation="MNELLIIAPIAAVILLGRLLGTSGAVSPQTFRETNKILYWISIP
                     ALLLRLTVRANLDALGGWNLFFAVYGTYFLLPLIAFGIGRLFKEDRKRLAISALTSMR
                     SNQVFMGIPAVSIAMGNPGLEALSLFLAMSLVGYHVISIASSQLVVTRELSVRSLLGT
                     ISKVVRNPMVCACLVGILCSSLGFREFPGWIDTTLKVLGDIGTGLALLALGASIKVVS
                     LKGVFRSTWRDCTIKLFLHPLTLFLFFSLLPVEKTMMQVVVFCSAMPVAVNSLVVAQG
                     MGMDEHYAAELIAVSTMLSVFTLPLWIRFLGI"
     gene            complement(1300..2184)
                     /locus_tag="PK442_05305"
     CDS             complement(1300..2184)
                     /locus_tag="PK442_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO87099.1"
                     /translation="MEKRHLKGSTFFFPGKVNVGYFQKNEEVWLVDTGLDDEAGRKIA
                     RFLETENKKLRCIVGTHSNADHCGGNAFLKKRTGCLIAATPLEAAVMENTFMEPLLLW
                     TAFPFAEISNRFLQAKPSSVDMLIRHSGVFDESGLEAVPLPGHFLEMIGVRTPDGVFF
                     VADALFSETLLEKYHIIFVLDVQAALETLTFLDKQEADWFVPSHAPAVEDIRPLAEAN
                     RRSLMRVSQAVLDSCSEPIGKDEIVKRVGALFGISMSITEFLLYSAAISAHLTWLRKN
                     ALISPFVEDGLLLWRRTP"
     gene            2349..2477
                     /locus_tag="PK442_05310"
     CDS             2349..2477
                     /locus_tag="PK442_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87100.1"
                     /translation="METVRKENQGEKVFAEKGEKDDRFDEVMADMNQAAYRALLGQ"
     gene            2595..>3248
                     /locus_tag="PK442_05315"
     CDS             2595..>3248
                     /locus_tag="PK442_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOO87101.1"
                     /translation="MSNNGNDSASSSFIPALEVRDLRKSFGNVSVLEGISLSVREGEV
                     ISVIGPSGSGKSTLARCICRLEEIDGGEVLLYGDRIDNGKHSNRDIARLVGMIFQQFN
                     LFPHLSVLENIILSPVRILGNSPKAAEDKAMSLLERVGLCEKKSARPNELSGGQRQRV
                     AIARALAMNPKIMLFDEPTSALDPELVGEVLDVIADLAQSGMTMMIIPDCARSAITSS
                     "
BASE COUNT          758 a          859 c          810 g          821 t
ORIGIN      
        1 gtacgtagca gcccctcgac aagagcgccc gaaattctcc gggtggaaac gcggacatcg
       61 cccccaacca cgatttttct tactccctga cggaccaacc aggttccgaa agcctgtcct
      121 atagctgaga ctacctggtc ggtcagatcg acatccgcta tgccgcgaat atcgtactcc
      181 cgaaaaatag tcggcgaaat atgcattcta cttcctcctt ttgacacgtc gctcttgtcg
      241 tccgcagcgt ctcgctgtat catcgaaacg cgtgtgcgta tacccttata atcagggtat
      301 acaaactaaa agtcaagatt cggtatgcgt attgcataga aaaggatgtg ctcctcgtga
      361 atgaactcct cataatagcg cctattgcag cagtgattct ccttgggaga ttgctcggta
      421 cgagcggcgc agtttctccg cagaccttcc gggaaacgaa caagattctc tactggatct
      481 ctattccggc gcttctgctc cgtctcacgg tccgcgccaa ccttgacgct ctcggaggat
      541 ggaatctttt cttcgcggtg tacggaacgt actttttgct accgctcata gccttcggaa
      601 tcggcagatt atttaaggag gatcggaaac gtcttgcaat atccgctctg acatccatgc
      661 gttcaaatca ggttttcatg ggaatccccg cagtatcaat cgctatggga aacccggggc
      721 ttgaagcgct gtcgcttttc ctggccatga gcctggtggg gtatcatgtc atttccattg
      781 cttcttccca acttgttgtt accagggagc tttctgtgcg ttctctcctc ggaacgatat
      841 caaaagtcgt acgaaacccg atggtatgtg cctgcctcgt cggaatactc tgctcctccc
      901 tcggcttccg tgagttcccc ggatggatag ataccacact gaaagtcctt ggcgatatcg
      961 gaaccggtct cgctctgctc gccctgggag cgagtatcaa agtcgtctcc ctcaaagggg
     1021 tttttcgttc cacgtggaga gactgcacaa tcaagctctt ccttcatccc ttgactcttt
     1081 tcctcttttt ctctctcctc cccgtcgaaa agacaatgat gcaggtcgtt gttttttgct
     1141 ccgcaatgcc ggtggccgtc aactctctgg tagtcgccca gggaatggga atggacgaac
     1201 actacgccgc cgagctcatc gccgtgtcga caatgctctc tgtgttcacg cttcccttgt
     1261 ggatccgttt tttagggatc tgaggaatta cgccgcgtgt cagggggtac gcctccacaa
     1321 gagcagcccg tcctcaacaa acggagaaat gagcgcgttt tttcgtaacc aggtcaggtg
     1381 cgccgatatc gccgcgctgt agagaaggaa ctccgtgatc gacatggaga tgccgaagag
     1441 agcgcctacc cttttgacta tttcgtcctt ccctattggt tccgagcagc tgtcaagaac
     1501 cgcctgactc accctcatca agctccggcg gttcgcttcg gccagaggtc gaatatcctc
     1561 aacggcaggg gcatgactcg gaacgaacca gtcagcctcc tgcttatcga ggaatgtaag
     1621 cgtctcaagc gcagcctgta cgtcgagcac aaaaataata tgatacttct ccagaagcgt
     1681 ttccgaaaaa agagcatccg caacaaaaaa cacaccgtca ggggtgcgta ctccgatcat
     1741 ttcaaggaaa tgcccgggaa gaggaactgc ttccagtccg ctctcgtcga ataccccgga
     1801 atgccgtata agcatatcca cagaggatgg tttcgcttgg agaaatctgt tcgatatctc
     1861 ggcgaaagga aatgcagtcc acagcagcag aggctccata aatgtatttt ccataaccgc
     1921 cgcctccaat ggggtggcag ctatcaaaca gcccgttcgc tttttgagaa aggcgtttcc
     1981 tccgcagtga tccgcatttg agtgcgtccc gacgatacag cggagttttt tattttctgt
     2041 ctctaaaaaa cgtgcgatct tccgccccgc ttcatcatcc aagccggtat ccacaagcca
     2101 gacttcttcg tttttctgga aatagcctac attcactttg ccagggaaaa aaaacgtcga
     2161 gcctttgagg tggcgtttct ccacttgatc acttcctttc gtgttttctt tattctctcg
     2221 tgaagaagaa atgtttgcaa gcctcttgcg tcgtgcgaaa aaagcgctat aataaaaaac
     2281 aactatggta cggaccataa atatattata cacaacagac tgtgttgcac tgtaagggag
     2341 gtaaaatcat ggaaactgtt cgcaaggaaa atcaaggaga aaaagtgttt gcggagaaag
     2401 gcgaaaaaga tgaccggttc gatgaagtga tggccgatat gaaccaggcg gcctacaggg
     2461 ctcttctggg gcagtagaac acgctcttcg tttcgggaga atatctgcgc gccggcattc
     2521 gccggctttt ctgttttttg ccgactcgtc ctccggtaga gtataataac ggcagctttc
     2581 gggaggtgag aggtgtgtcc aacaacggca acgatagtgc gtcatcatcg tttatacccg
     2641 ctcttgaggt gcgggatctt cggaaatcat tcggcaatgt atctgttctt gagggcatct
     2701 cgctttccgt ccgtgaagga gaagtcatct cggtgatagg tccgagcggt tcaggaaaga
     2761 gtacgcttgc acggtgtatc tgccgtcttg aggaaatcga cgggggggag gttttgctct
     2821 acggagacag gattgacaac ggcaagcatt cgaatcgtga tattgcccgc ctcgtaggga
     2881 tgatttttca gcagttcaat cttttccccc atctgtcagt tcttgagaat atcattttga
     2941 gccctgtccg cattttggga aactctccga aggctgcgga agataaagcg atgtcgcttc
     3001 tggagcgtgt aggactctgc gagaaaaagt cagcgcgtcc caacgagctc tccggaggac
     3061 aacgtcaacg tgtagcaatc gcccgcgccc tcgcaatgaa tcccaagatt atgctcttcg
     3121 atgagccaac cagcgccctc gacccggaac ttgtggggga ggtgcttgac gttatcgccg
     3181 atctagcaca gtccggaatg accatgatga tcattccgga ctgtgctaga tcggcgataa
     3241 cgtcaagc
//
LOCUS       DAOSWW010000206         3247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21438_length_3247_cov_3.965539, whole genome shotgun sequence.
ACCESSION   DAOSWW010000206 DAOSWW010000000
VERSION     DAOSWW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21438_length_3247_cov_3.965539"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2312)
                     /locus_tag="PK442_05320"
     CDS             complement(<1..2312)
                     /locus_tag="PK442_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOO87102.1"
                     /translation="MPSLCSLKKTLFRSSAPLIAGVVSLLFILGCVSRADAGQQKKNI
                     LVLHSFHQGYEWTDSVQQGILDELAPRRKDVSLSVEYLDSIRSPEASQLEAARELFRR
                     RYAHGKTLFDVILCSDDEALGFLLEYGGELFGDVPVVFCGVNNFSQGRLGGRKNITGV
                     NEAISVRETIEIALKLRPSAKTVAVVAGFRPAEIRNLEMAKAAEPFFKGRVAFEYLHG
                     FEPEEIANRLKELSSEDIVLYLSYLLSPSGKRLSVEENRTFVVGATDAPVFSCWDTLL
                     SYGFVGGKVVHGRSQGEIAASLALQILDGKRAEDIPVVFDSPNRYAFNASALKTFGVP
                     ESLLPPYSFLLQTTGDGLAATWDELVVRRFFSEELFSRHGAPMLIIDPESGLILAGNQ
                     AAHSFYGYPELQGMTIGSINALSSQQVQDEMQKATRLRKNHFDFRHRLADGSVRDVRV
                     YSYPIELDGREVLFSMVIDETERTIAEASLHRKERSFITVLSVAVLLQLAALFLLARS
                     AARRKKAEKELAEAYENQSILLDNIPTQIWLLTDDHTYGAVNRAHADFNGMRAEEMSF
                     RDMYEFLPGEVVEVCRRSNAEVFADARPVRTDEKIRDASGEERLLSIWKTPRLRADGS
                     VEYVICAGEDVTEQKRTEDSLRAALRELEQKTELANEMAERAQAANVAKSAFLANMSH
                     EIRTPMNGILGMTGLLLETGLTQEQRGFAGSLMSSAESLLSLLNDILDFSKIEAGKLE
                     LETLDFNLETLLKDFTSVMAVVARKKGLELR"
     gene            2590..>3247
                     /locus_tag="PK442_05325"
     CDS             2590..>3247
                     /locus_tag="PK442_05325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO87103.1"
                     /translation="MRRDYLRMLDTLKEGVYFVDLDRKITFWNKGAERITGFRREEVV
                     GSSCMDNILVHVDCKGTQLCKEGCPLLYTMKDGSSREAEVFLHHRRGHRVAISVRTTP
                     LEDDEGNIIGGIELFSELNPEESLRQRMEELERCAMIDPLTGVPNRSYLNPELERLFR
                     LWEANRIPFGLLFFDIDHFKRFNDTYGHDVGDQVLRTVAQSLSSSVRNYDSVGRWGGE
                     E"
BASE COUNT          729 a          962 c          901 g          655 t
ORIGIN      
        1 cgcagttcca gccccttctt ccgggcaacg acggccatca cggaggtaaa atccttcagg
       61 agcgtctcca ggttaaaatc aagcgtttcc agctccagct ttccggcctc aatctttgag
      121 aagtcgagaa tatcgttgag aagagagagc agcgactcgg cgctcgacat caaactcccg
      181 gcaaaccccc gctgctcctg cgtcaaaccg gtctccagca gcaggccggt cattccaaga
      241 atgccgttca tgggcgtgcg tatctcgtgg ctcatattcg ccagaaacgc gcttttcgca
      301 acgtttgccg cctgcgcgcg ctcggccatc tcgttcgcca gctccgtctt ctgttcgagt
      361 tccctcagcg cagcgcgcaa cgaatcctcc gttcgctttt gctcggtaac gtcctccccg
      421 gcgcagatga catactccac actgccgtcg gcacggagcc gcggcgtttt ccaaatggag
      481 agaagccgct cttctccgga agcgtcgcgg atcttctcgt cggtacggac agggcgggcg
      541 tcggcaaaga cctccgcgtt cgatctccgg cagacctcga cgacctcgcc cggcagaaat
      601 tcgtacatgt ccctgaacga catctcctcc gcccgcatcc cgttgaagtc ggcgtgcgca
      661 cgattcaccg cgccgtatgt gtggtcgtcg gtcagaagcc agatctgcgt cggaatgttg
      721 tcgagaagga tactctggtt ttcgtaggct tctgcaagct ctttctccgc tttctttctc
      781 cgcgcggcgc ttctcgcaag gagaaagagc gccgcaagct gcaagagtac cgcaacggac
      841 aacaccgtga taaacgaacg ctccttgcga tgaagagagg cttctgcgat cgtcctctcc
      901 gtttcgtcga tcaccatgga gaagagtacc tctctcccgt cgagctcgat cgggtaggag
      961 taaacccgta catcgcgcac cgaaccgtcg gcaagcctgt ggcgaaaatc gaagtgattc
     1021 ttccgcagcc tggtcgcttt ttgcatctcg tcctgcacct gctgcgaaga gagtgcattg
     1081 atgctgccga ttgtcatccc ctgtagctcc gggtaaccgt agaagctgtg cgctgcctga
     1141 ttccctgcca gaatgagtcc gctctccgga tcgatgatga gcatgggcgc cccgtgtctg
     1201 gagaagagct cttcgctgaa gaagcggcgg acgacaagct cgtcccatgt cgcggccaat
     1261 ccgtccccgg ttgtttgaag aagaaacgaa tacggcggaa gaagcgactc gggtacgccg
     1321 aacgttttga gcgcggaggc gttgaaggcg taccggttgg gactgtcgaa aacgaccggg
     1381 atatcctccg ccctttttcc gtccagaatc tgcagcgcaa gcgacgcggc gatctccccc
     1441 tgagaccgcc cgtgcaccac ctttccgccg acgaaaccgt acgaaagcag cgtatcccag
     1501 cagctgaaga cgggggcgtc cgttgccccg acgacgaacg ttctgttctc ctcgaccgag
     1561 agccttttcc ctgaagggga gaggaggtag gagaggtaga ggacgatatc ttcggatgaa
     1621 agctccttca gccgatttgc gatctcctcc ggttcgaaac cgtgcaaata ctcgaacgcc
     1681 actcttccct tgaagaaagg ttccgcggcc tttgccatct ccaggttgcg aatttcggcg
     1741 gggcggaaac cggccaccac ggcgaccgtc ttcgcggaag gacgaagctt cagggcaatc
     1801 tctatcgtct ccctgacgga gatcgcttcg ttgacgcccg taatgttttt ccttccgccg
     1861 agacgcccct gtgaaaagtt attgaccccg cagaagacga cgggaacgtc gccgaagagc
     1921 tctccgccgt actccagaag aaagccaagc gcttcgtcgt cgctgcagag gatgacgtcg
     1981 aaaagcgttt tcccgtgtgc gtaccggcgg cggaagagtt cgcgcgccgc ctccagttgg
     2041 ctcgcctccg gagaacgtat ggagtccaga tactccacgg agagcgatac gtccttccgc
     2101 cggggcgcca gctcgtcgag gatgccttgc tggacggaat cggtccactc gtacccctga
     2161 tggaacgagt gcagcacgag gatatttttc ttttgttgcc cggcgtcagc ccgcgaaacg
     2221 catccaagaa tgaagagcag cgacacaacg cccgcgatca gcggtgctga agaacgaaac
     2281 agagtttttt tcaaggaaca aagagatggc aagaacacct cttcaccagc tttcagttat
     2341 aataaaagcg tcaataagcg ctatggcgtg cggcttccta aaaagcgaat tgtcatgtac
     2401 tattcatacc aagcatttgt aaatctcact atatcgcaca aaatctttga tgtaaagaaa
     2461 acatcgggaa agaacacacg aagacgctgc ctggagcaaa cggtgaatcg gcgcaaacaa
     2521 aaccagagta tccttctgta ctttttagaa ataaaattga aaacagcctc ctacgaaagg
     2581 atgaaaagaa tgcggcggga ctatcttcgg atgctggata cgctcaagga aggagtctac
     2641 ttcgtcgacc tcgacagaaa gatcaccttc tggaacaagg gcgcggagcg aatcaccggg
     2701 tttcgcagag aggaagtcgt cgggagctcg tgcatggaca acatcctcgt gcacgtcgac
     2761 tgcaagggaa cccagctttg caaagagggg tgtccgctcc tctacaccat gaaggacggc
     2821 tcctccagag aggccgaggt ttttctgcac cacaggagag gacacagggt cgccatctcc
     2881 gtgcgcacaa ccccgcttga ggacgacgag ggaaacatca tcggcggcat cgagctcttc
     2941 agcgagctca accccgagga gagcttgcgt cagcggatgg aggagcttga acggtgcgcg
     3001 atgatcgacc cgctcaccgg cgtgccgaac aggagttacc tgaatccgga gctggaacgc
     3061 ctcttccgct tatgggaggc taaccgcatc ccgttcggac tgctcttctt cgatatcgac
     3121 cacttcaagc ggttcaacga cacctacgga cacgacgtcg gcgaccaggt gctgcgaacg
     3181 gtcgcgcaat cgctctcatc ctccgtcaga aactacgaca gcgtcggacg ctgggggggc
     3241 gaggagt
//
LOCUS       DAOSWW010000207         3237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21544_length_3237_cov_4.549026, whole genome shotgun sequence.
ACCESSION   DAOSWW010000207 DAOSWW010000000
VERSION     DAOSWW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21544_length_3237_cov_4.549026"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PK442_05330"
     CDS             complement(<1..729)
                     /locus_tag="PK442_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOO87104.1"
                     /translation="MVHKIVRYSVLLFILVPLLTWFASRGADMVALARWNTEDSAIVI
                     LEKVLGREDDVESGAEGNWETERVHIRITYRNGAFAGTTEEIEILQLADSRLELVPGR
                     EYLFLVDTFDDGTHQYSISDRYRIPAVAGFITLACAALAVLAGTSGVKALCGLFLSLF
                     LLLGWFVPMLAEGYTPVPFAILAIAGVSLLTIVFVVPKRALWPIPFIGAVGGAIGASV
                     TGWIMVGLWQLTGLENDSAVLLFSV"
     gene            complement(756..2597)
                     /locus_tag="PK442_05335"
     CDS             complement(756..2597)
                     /locus_tag="PK442_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOO87105.1"
                     /translation="MEMKIARVNLTESTIEIFSAEDELLRKYLGGSGLGTYYLFKYGS
                     PKCAALSPENPLLFMNGPFQGTGIPTSGRHQVLAKSPLTDMFGESDCGGAFGYHMRKA
                     GFDGIILLGKAPSPVYIALLDGKIEIRSASSLWGKDTFETEEFLLELEGKPAGVACIG
                     PAGENQVLLANIMHDGRNARAAGRGGMGAVMGSKNLKAIVARGSAKVLMADEKAVREK
                     SAEKAKYYMEKLPAMTRFGTAGGVALAEQNGDLPLKNWSMGSWTEDVKSITGEAMAET
                     ILTGNWGCMSCPIRCGRDVEFDGIKGAGPEYETIGMLGSNCLISDLKAIARGNDLCNR
                     MGIDTISGGSAVAFAMELYERGIISEEEIGYPLLWGDGESMIRLLSDIVEKRGFGALL
                     SEGTKKAAERIGKGAERYAIHSKGMDFPAHDPRCYKSLATGYATSNRGACHLSGFSYS
                     FERSMTYPEFGVDTVMDRTVDEGKGAVNIPFQDMMGVLDSLKMCKFPFAITRIDDILQ
                     WINGITGWNMSGEELLLAGERIFNLKRLFNVACGASALDDTLPERILTEPRGSGGSAN
                     TLPDLKLQLKEYYPERGWSEDGIPTEEKLKSLDLEEFLCCVNGPALE"
     gene            complement(2624..3157)
                     /locus_tag="PK442_05340"
     CDS             complement(2624..3157)
                     /locus_tag="PK442_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOO87106.1"
                     /translation="MSVLGTLALVFARIGLGAFGGGLATIPFIHFELVASRAWLSERG
                     FAEVVSLAQMTPGPLAVNAATFVGYRIAGVAGASVATFSVIAAPLLVLGLALFLISRA
                     SEKWKTRVDRLQRALRPAVSGMLFAAFWMVARSLTGDWRLWVMTAGVFMLIGQEYFKR
                     YPQLLLLIAGMAGVLFL"
BASE COUNT          799 a          962 c          763 g          713 t
ORIGIN      
        1 aacggaaaaa agaagcacgg cgctatcgtt ctccagtccg gtcagctgcc acagcccaac
       61 cataatccag cccgtaaccg aggctccgat tgctcctcct acggccccga tgaaaggaat
      121 tggccaaaga gcgcgtttgg gaacgacgaa gactatcgtc agcagagaaa ccccggctat
      181 ggccaagata gcgaaaggca caggcgtata cccttcggca agcatcggaa caaaccatcc
      241 aagtaacagg aaaagcgaca gaaacagacc gcagagagcc tttacccccg acgtacccgc
      301 aagaacggca agcgctgcgc aggccaaggt gataaagccg gcgacagcgg gtatcctgta
      361 tctgtcgctt atagaatact gatgtgttcc gtcgtcgaac gtatcgacaa gaaagaggta
      421 ttctctccca ggaacaagct ccagccggct atccgcaagc tgaaggattt ctatctcctc
      481 agtcgtgccg gcgaacgcac cgttacggta ggtaattcgt atgtgtacgc gctctgtctc
      541 ccagttgcct tcggctccac tttcaacgtc gtcctccctg ccgagtacct tttcaaggat
      601 gacgatggcg gaatcttccg tattccacct ggcgagcgca accatgtcgg ctcctcgcga
      661 cgcaaaccag gtgagcagcg gtacgagaat aaacaataga acggagtaac ggacaatctt
      721 atgtaccata attcactcct tttttccaaa gcgtatcatt cgagagcagg cccgtttaca
      781 cagcataaga actcttccaa atcaagagat ttcagttttt cctcggtagg aataccatct
      841 tcagaccagc ctctttcagg ataatactct ttgagctgca acttcagatc cggaagagtg
      901 ttcgccgaac cgccgcttcc gcgaggttcc gtaagaattc tctccggaag agtatcgtca
      961 agcgcggaag cgccgcaggc tacattgaaa agccgcttga ggttgaaaat cctctctccc
     1021 gcaagaagaa gctcttcgcc actcatattc caccccgtga ttccgttgat ccattgaaga
     1081 atatcatcta tccttgtgat tgcgaacggg aacttgcaca ttttcaagga atcaagaaca
     1141 cccatcatgt cctggaaggg aatattcaca gcccctttcc cttcatcgac cgtcctatcc
     1201 atcaccgtat caacgccgaa ctccggatag gtcatgctcc gttcaaacga ataggaaaaa
     1261 ccggaaagat ggcaggctcc ccggttcgat gttgcgtatc ccgttgccaa gctcttataa
     1321 cagcgggggt cgtgcgcggg aaaatccatt cccttggaat gaatggcata ccgttccgcc
     1381 ccttttccga tacgctccgc agcctttttg gttccctcgg acagcagcgc gccgaatcct
     1441 cgcttttcta caatatccga tagaagacgt atcattgact ccccgtcacc ccacaaaagc
     1501 gggtagccaa tttcctcttc cgaaattatc cctctttcgt acaactccat ggcgaaagcg
     1561 acagccgaac ctcctgaaat cgtgtcgata cccattcggt tacacaggtc gtttccacgc
     1621 gcaatggcct tcagatcgct gatgaggcag ttgcttccca gcataccgat cgtttcatac
     1681 tccggcccgg cgccctttat tccgtcgaac tcgacgtccc ggccgcatcg tatcgggcag
     1741 gacatgcagc cccagttgcc ggtcagtatc gtctcggcca ttgcttcacc ggtaatggac
     1801 ttcacatctt ccgtccagga gcccatactc cagttcttca ggggaagatc tccgttctgc
     1861 tcagccaacg caactccccc ggcggtaccg aaacgggtca tcgcgggaag cttttccata
     1921 tagtacttcg ccttttctgc agacttctct cggacagcct tttcgtccgc catgagcact
     1981 tttgcgctgc cgcgggcaac tatggctttc aaatttttgg agcccattac cgcccccata
     2041 ccgcctcttc ccgcagctct ggcatttcga ccgtcgtgca tgatatttgc aagcaagacc
     2101 tgattctctc ctgcaggacc tatacaggca acaccggccg gttttccctc cagctccaac
     2161 aaaaactcct ccgtctcgaa cgtgtccttt ccccagagag aggacgcgct gcgaatctcg
     2221 atttttccgt caaggagggc tatgtacacc ggagagggcg ccttgccaag aagaatgatg
     2281 ccatcaaaac cggccttgcg catatgatac ccaaaagcgc ctccgcagtc ggattctccg
     2341 aacatatcgg taagcgggga tttcgcgagc acctgatgtc ttcccgaagt aggaattcct
     2401 gtcccttgga aaggaccgtt catgaaaagc agaggatttt caggggagag cgccgcgcac
     2461 ttcggcgagc cgtacttgaa aaggtagtac gtcccaagtc cggatcctcc aaggtacttt
     2521 cgtaaaagtt cgtcctcggc agagaaaatt tctattgtcg attccgtgag attaacgcga
     2581 gcgattttca tctccattct ccatcatccc tttctttttt cgttcagagg aagagcactc
     2641 cagccatccc cgcaataagg agcagtaatt gaggatatcg cttgaagtac tcttgcccga
     2701 tgagcatgaa tactcctgct gtcatcaccc agagcctcca gtctcctgtc agagagcggg
     2761 ctaccatcca gaaggcggcg aagagcattc ccgatacagc agggcgaagt gcacgctgta
     2821 atcgatcgac tctcgttttc catttctccg atgctctgga aatcaggaaa agcgccaagc
     2881 cgagcacaag aagcggagct gcaatgacag agaatgttgc aacagatgcg cccgcaacgc
     2941 ctgcgatcct gtagccaaca aaggttgcag catttacggc gagagggccg ggcgtcatct
     3001 gcgccaatga aacaacttca gcgaagcctc tctctgaaag ccacgctctg gaggcgacaa
     3061 gttcaaaatg aatgaagggt atggtcgcca agcctccgcc gaacgccccc aggcctatgc
     3121 gggcaaaaac aagtgcgaga gttccaagaa cactcatccg gcatcctctt ccccgaggag
     3181 cagacgaaca gtcactcccc ccgcgagggc aaaaaacggg tgtacatcag tgctgat
//
LOCUS       DAOSWW010000208         3233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21593_length_3233_cov_3.051919, whole genome shotgun sequence.
ACCESSION   DAOSWW010000208 DAOSWW010000000
VERSION     DAOSWW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21593_length_3233_cov_3.051919"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            603..968
                     /locus_tag="PK442_05345"
     CDS             603..968
                     /locus_tag="PK442_05345"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOO87107.1"
                     /translation="MESILSISKALGEKNRLRVFLALIHTKELCVCEVGELLGLAPAT
                     VSRHMSILSRAKLVDSRKRGKWVYYCIKPAIAPELLQWIKNSVVDSFSVAEDVKRIEI
                     LVRERQDKDCTKACVSEER"
     gene            975..1430
                     /locus_tag="PK442_05350"
     CDS             975..1430
                     /locus_tag="PK442_05350"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOO87108.1"
                     /translation="MSKKPKVLFLCTGNSCRSQMAEGWTRHFHSAAIEAHSAGIETHG
                     LNPFAVEAMKEAGADISGQRSKNVMELLDVSFDYVVTVCGDADERRPFFPGETRVLHV
                     GFDDPPKLALLEETEEGKMNCYRRVRDEIRAFIKDLPGFLENLPEEKRS"
     gene            1438..2244
                     /gene="arsM"
                     /locus_tag="PK442_05355"
     CDS             1438..2244
                     /gene="arsM"
                     /locus_tag="PK442_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOO87109.1"
                     /translation="MDDIRNYVKERYAQAIKNKSSCCGSGGCCCGTTGASGIPSMTRG
                     NYDGATLEKTPEQMADQSFGCGNPFTEARIHPGETVLDLGSGAGLDLFLASEMTGPYG
                     KVIGLDMTDEMLATAAENLKGIENVSLVKGYIEEMPVESDSVDVIISNCVINLSPDKG
                     KVLKEAFRVLRPGGRFCVSDTVFLRPVTERAVKNLAAWSGCISGALRETEYAEKMREA
                     GFEEVEVRRTKVYSFPDAMAAMAFPELSGEERNEINGSLASAIIMGKKPL"
     gene            2399..>3233
                     /locus_tag="PK442_05360"
     CDS             2399..>3233
                     /locus_tag="PK442_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO87110.1"
                     /translation="MKRPIAFALVLTIVCFLSGSAWSANGKFNAESYSSPKIRDLYLV
                     IIRDPDAQLLALQRGQADILGDLHRPVDVDTLADDPRVNLSLASGFHGFFLGFNVRTF
                     PWNRLELRQAAWQALPRERVVRDLFAGYAEPLSTFLPPVSPYYEPNVAAYPFDPDAAK
                     KRLADAGWTWGEDGVLISPEGEKIPDQRLLSPTASVAPTTAELAARAADALSAIGIPV
                     RSEPMDFSTMLSRLDERNFQMYVIAWQMTRDPDSLYALYSSKMDIPGGYNIPGISNPE
                     LD"
BASE COUNT          727 a          915 c          938 g          653 t
ORIGIN      
        1 cggacgtacg ctcacaaatc tcccgatggt taaggggatt cgtgaaaacc ttaaagggaa
       61 actttaccgg agcatgtcgg cggcgaaggg gaaaatcata aatcatgtcg cgctggtgaa
      121 gataataata cctttctaac gaaagatatc cacggcgtcc actacgcttc gccattcctt
      181 atttgtcccg gcagggctcc ggggaaatcg ctccttgccg cacaccaccg cttgaagcga
      241 cgaaatgccg cgaaaggatt gggttgtttt accggagccg gcacggtctt ctgattctcg
      301 cgcaatcttc ggaagtatgt tgctgcttca cccgtgcgat gatataccat ttttcgccac
      361 attgtcaacg agtgaaataa aaaaaaggaa attgtaaatg agctaacata atttacggta
      421 tacattttaa tgtaagtatg tgcggatacg cctctaactt gtacaaaatg catgatgtct
      481 cttcgatttg acgagcgctc acgtccagga aaagaaacta cccgacgaag cctcttgaca
      541 aagagggcgc ccccgcttat catttcgtca tgtgtaaaat taacgaaata aaggagtcag
      601 ctatggaatc catactttcc atttcaaaag cgctcggaga aaagaacagg ctccgcgtct
      661 ttctggcgct tatccacacg aaggaacttt gcgtctgcga ggtgggggaa cttctcggcc
      721 ttgcccccgc gacagtgtcg cgccacatga gcattctctc ccgggcaaag ctcgtcgact
      781 cccgtaaacg aggcaaatgg gtctattact gtatcaagcc tgcgatagcg ccggagttac
      841 tccaatggat aaaaaattcc gtcgtcgatt ctttttccgt tgcggaagac gtgaagagaa
      901 tcgagatcct cgtcagggaa cgccaggaca aagactgtac aaaagcctgt gtatccgaag
      961 agaggtgaaa taacgtgagc aaaaagccaa aggtgctctt tctctgcacc gggaactcgt
     1021 gccgcagcca aatggccgag gggtggaccc gtcatttcca tagcgccgcc atagaagcgc
     1081 attctgccgg aatcgagacg cacggcctga atccgttcgc cgtggaggct atgaaggagg
     1141 cgggagcgga catctccgga cagcgctcca aaaacgtgat ggagctgctc gacgtttcat
     1201 tcgactacgt cgtcaccgtg tgcggcgacg cggacgagcg gcgcccgttc tttccggggg
     1261 agacgagagt cctccacgtc ggcttcgacg acccgccgaa gctggcgctc ctcgaagaga
     1321 cggaggaggg aaaaatgaac tgctaccgcc gggtgcgcga cgagatacgc gcattcatca
     1381 aagaccttcc cggctttctt gaaaatctac cggaggaaaa aaggagctag atcaagtatg
     1441 gacgatattc gcaactacgt gaaagagcgg tatgcacagg cgatcaagaa caagtcctcg
     1501 tgttgcggct cgggagggtg ctgctgcgga acgacgggcg catccggcat cccgagcatg
     1561 acgcgcggca actacgacgg cgcgacgctc gaaaaaactc cggagcagat ggccgaccag
     1621 tccttcggct gcggcaatcc gttcaccgag gcgcgtatcc atccgggcga gaccgtcctc
     1681 gacctgggaa gcggcgcggg gctcgacctc tttctggcgt cggagatgac cggcccttac
     1741 ggcaaggtca tcgggctcga catgacggac gagatgcttg cgaccgccgc cgaaaatctc
     1801 aaggggatcg aaaacgtctc cctggtgaaa gggtacatcg aggagatgcc ggtcgagagc
     1861 gactcagtcg atgtcatcat ttcaaactgc gtgatcaacc tttccccgga caagggaaag
     1921 gtgctgaagg aggctttccg cgttctccgt cccggcggga ggttctgcgt ctccgacacc
     1981 gtcttcctcc gtcctgtgac ggagcgcgcc gtcaagaacc tggcggcgtg gtccgggtgc
     2041 atttccggag cgctccggga gacggagtac gcggagaaga tgagggaggc gggcttcgag
     2101 gaggtcgaag tacggcgcac gaaggtgtac tccttccccg acgcaatggc ggcgatggcc
     2161 ttccccgaac tctccggcga agaacgaaac gagatcaacg gctcgttggc cagcgcgatc
     2221 atcatgggga aaaagccgct ttagggcgtt cgtcaggttc gcggaaatca gaaagcgccg
     2281 gtaccggggg actccctctt ctggttccgg cacttttttt cagacgttgt ttcgccgcgt
     2341 cttctggtaa aatacttgaa aacatactcc tcagggatac gggaaggatg ggaaagagat
     2401 gaagagaccg atcgccttcg cacttgtcct tacaatcgtc tgttttcttt ccggaagcgc
     2461 atggagcgca aacggaaagt tcaacgcgga gagctactcc tctccgaaaa tccgggatct
     2521 ttacctggtg atcatcaggg atcccgacgc ccagcttctg gcgcttcagc gagggcaggc
     2581 ggatatcctc ggagatctgc accgccccgt cgacgtcgat acgctcgccg acgatccgag
     2641 ggtgaacctc tcgctcgcat ccggttttca cggcttcttc ctcgggttca acgtccgcac
     2701 cttcccgtgg aaccggctcg aactgcggca ggcggcgtgg caggcccttc cgagggagcg
     2761 cgtcgtgcgc gatcttttcg cggggtacgc ggagccgctc tcgacgtttc ttcccccggt
     2821 ctccccgtac tacgagccga acgtcgcggc gtatccgttc gaccccgacg cggcgaagaa
     2881 gcgcctcgca gacgccgggt ggacatgggg ggaggacggg gtgctgatct ccccggaagg
     2941 tgagaaaatc cccgaccagc gtcttctctc gccgacggcc tccgttgcgc caaccactgc
     3001 ggagctcgcg gcgcgggctg cggacgcgct ttcggcgatc ggcattcctg tgcgttcgga
     3061 gccgatggac ttctccacca tgctttcgcg gctcgacgag cggaacttcc agatgtacgt
     3121 catcgcgtgg cagatgacgc gcgatcccga ctccctctac gcactttact catcgaaaat
     3181 ggacattccc ggcggataca acattcccgg catttccaat ccggaactgg acg
//
LOCUS       DAOSWW010000209         3207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_21893_length_3207_cov_4.000000, whole genome shotgun sequence.
ACCESSION   DAOSWW010000209 DAOSWW010000000
VERSION     DAOSWW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21893_length_3207_cov_4.000000"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK442_05365"
     CDS             complement(<1..193)
                     /locus_tag="PK442_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO87111.1"
                     /translation="MENVKVKTKIYDQVVDRIKQRISEGELPQGSALPPERQLIDELN
                     VSRSSLREGFRILEMMGMIE"
     gene            287..1933
                     /locus_tag="PK442_05370"
     CDS             287..1933
                     /locus_tag="PK442_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOO87112.1"
                     /translation="MAFTIEVANGPVFSSDVPVTGKSVLSESRPDGEEKTIVAWRVNN
                     YLRSLDWVIEDNARVEFIDTSSFEGMEVYRRSLSFLLVLACKRGLAKDVVLRHSISEG
                     YYWEFVEGEVTDEDLAVIKATMADLVGKDLPFIRKVVPLDKARRIFDRQGSGEVARLF
                     QWGAVDPVELYRCADMYGYYYAPLAPSTGYLKLYDLKKLGPGMALQFPTISYPSSLPP
                     FRASKNLSGVFLDYAKWLEVLDLRTMDSLHKKVAEGRSQEVILIAEAFHAQKLGRIAD
                     EIASKTKVKVAAIAGPSGSGKTTFSERLKIQLLVCGRNPVTLAMDNYFVNREDTPRDE
                     QGNLDFEVVEALELELLEDHLRRLLAGEEVVTPQFSFIEGKKKPGKKIKLGPRDILIM
                     EGIHGLNDRVTGAIAKDELYGIFVSPLTGVNFDNHNRTSTTDNRLLRRLVRDARTRGH
                     SAESTLTLWPKVIRGSMQFVFPYQKHADVMFNSSLPYELAALRAYVDPILHTVPENSP
                     VYGEAQRILSMIKFVPMIPTEIIPNNSIIREFIGGSCFDV"
     gene            1965..2804
                     /locus_tag="PK442_05375"
     CDS             1965..2804
                     /locus_tag="PK442_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO87113.1"
                     /translation="MEDVARFAGVNKATVSRALKGDPRISSVTSEKVWNAAKTLGYRI
                     DRTASGLSGGKTGLAAVVLESLRPWFSPLFFSGLNRVLGNAGMDILLKMPGFSDDALE
                     ASLVSRRVDCILLAGSGEGMAPSRFLLPQIPVVTAGFSIAETPSVFVSEQETLSRLRS
                     VSSGRPLRFVSGTSPLFPFLSRSITDSEAAPEEYFWIMDGCAPEDVGRTVRFGCVCTL
                     PEEPVRTGFFRIEWPAFEMGVAVGRLLMKASSGKDVFPTRLMPVPLVRDPAGEKILFG
                     KMN"
BASE COUNT          738 a          813 c          847 g          809 t
ORIGIN      
        1 tctcgatcat gcccatcatt tcgagaatgc gaaacccttc ccgaagcgaa ctgcggctga
       61 cgttcagttc gtctataagc tggcgttccg gcggaagagc ggaaccttgg ggaagttccc
      121 cctcggaaat acgctgttta attctatcga ccacctggtc gtatattttc gttttcactt
      181 ttacgttttc catgtgtgtc tccttcctgt gttccgtatg gtaatataag cataatctac
      241 tttcaaatac agcgcaaccc gtttatcgaa gggaggagaa cgttctatgg cattcactat
      301 tgaagttgca aacgggcctg ttttttccag cgacgttccg gtcacaggga aatccgttct
      361 ttcggagagc cgccccgacg gcgaagagaa gaccatcgtc gcgtggagag taaacaatta
      421 ccttcgttca ctcgactggg ttattgaaga caatgcacgg gttgagttca ttgatacatc
      481 ctcctttgaa ggcatggaag tgtatcgccg ctccctgagc ttcctcctcg tgcttgcatg
      541 taaacgaggc ttggcgaaag acgtcgttct ccgccactcc atcagtgaag ggtactactg
      601 ggagtttgta gagggagaag tcacggatga agaccttgcg gtcatcaagg ccactatggc
      661 tgatcttgtc ggaaaggatc tgccgtttat tcgaaaagtt gtccccctcg ataaggccag
      721 gagaattttc gaccggcagg gaagcgggga agttgcacgg ctctttcagt ggggcgccgt
      781 cgatcctgtg gaactctatc gttgcgcaga tatgtacgga tactactatg ccccgctcgc
      841 cccgtcgacc gggtatttga agctctacga tctcaaaaag ctcggacccg ggatggcgct
      901 tcaattcccg acaatcagct atccttcctc tctgcctcca ttccgcgctt cgaaaaatct
      961 ctccggagtt ttcctggact atgctaaatg gcttgaagtg cttgatctcc ggacaatgga
     1021 cagtctgcat aaaaaggttg cggagggcag aagtcaagaa gttatcctta tcgcagaggc
     1081 attccatgcg cagaaattgg ggcggatcgc cgatgaaata gcctcaaaga caaaagtgaa
     1141 ggtagcggcg attgcagggc cttcaggctc cggaaagaca actttttccg aaaggctgaa
     1201 aatacagctt ctcgtctgcg gcagaaatcc cgtgacgctt gctatggata actactttgt
     1261 gaaccgggag gatactccgc gcgacgagca aggcaacctc gattttgaag tagtggaggc
     1321 gctcgaattg gaactgctgg aggaccacct tcgccgcctt ctcgccggag aagaggttgt
     1381 cacccctcag tttagtttta tcgagggaaa gaaaaaaccc ggtaaaaaaa taaagctcgg
     1441 tccccgtgat atcctgataa tggagggaat acacggcttg aatgacaggg tgaccggcgc
     1501 gattgcaaag gacgaactgt acggaatttt cgtctctccg ctcacaggtg tgaacttcga
     1561 taaccataac cggacgagta ccacggacaa ccgtcttctt cgccgtcttg tacgcgatgc
     1621 aagaacgaga gggcattctg ctgaatcaac gttaaccctc tggccgaagg tcatcagggg
     1681 gagcatgcag tttgtgttcc cctaccagaa acatgcggac gtgatgttta actcctctct
     1741 accgtacgaa ttggcggctc tgagggcgta tgtcgatccg atacttcata cggtcccgga
     1801 aaactcccct gtctacggtg aagcgcagcg aattctctcg atgatcaagt ttgttcccat
     1861 gattccaacg gagatcatac cgaacaactc gattatcagg gagttcatcg gcggctcatg
     1921 tttcgacgtg tagcgccctc ctccctattg tgacgcgagc gacgatggaa gatgtggccc
     1981 ggttcgccgg tgtgaacaag gccacagtca gcagagcgct gaaaggagat cctcgcatct
     2041 cttccgtcac gagtgaaaaa gtgtggaatg ctgcgaagac tctcgggtat cgtattgacc
     2101 gtaccgcaag cgggctttcc ggaggcaaga ccggccttgc ggcagttgtg cttgaaagcc
     2161 ttagaccctg gttcagtccg cttttctttt ctggattgaa ccgtgttctc ggcaacgcgg
     2221 gaatggatat tcttctcaag atgcccggtt tcagcgatga cgcgctggaa gcttcgcttg
     2281 tatcacgccg tgtcgactgt attcttcttg caggaagcgg ggaggggatg gcgccctccc
     2341 gctttttgct accgcagata cctgttgtaa cggccggttt ttccattgct gaaacacctt
     2401 cagttttcgt ttcagagcaa gagacgctct cacggcttcg ctccgtctct tcggggcgtc
     2461 cgcttcgttt tgtttcggga acgtctcctc tttttccctt tctctcacgc agtatcacag
     2521 attcggaagc agccccggag gaatactttt ggatcatgga tggttgcgca ccggaggacg
     2581 tcgggcgtac ggttcgtttc ggatgtgtct gtactctccc tgaggaaccg gtacgtacag
     2641 gtttctttcg tatcgaatgg ccggcttttg aaatgggagt tgccgtcggg cgccttctta
     2701 tgaaagcgtc gtcggggaaa gatgtcttcc caacccgcct aatgcctgtt ccgcttgtcc
     2761 gggatcccgc gggagaaaaa atactcttcg ggaaaatgaa ctaaggtttt tttgaataga
     2821 aaagctaaac gggtgttgac ttatgaaaaa agtcgtggta taacatcctc gtgaaagaaa
     2881 cagtgtcagt cgctgccaag cagacaaaaa cacgaggagg ttttcaacat gtggatgaaa
     2941 gatacaaacg ggaattggtt ctgtgacgct cctctttgca gcggatgctg caccccatcc
     3001 ctcgtatggg ataaaaatga aaatggcgcg tggttccttt cggaacccgg cgccatctct
     3061 gcggcaatat catcatttcc tgtaaaacac cagctctccc tggcggtgta gcttaaaaac
     3121 cggaggttac ctggcatcct cccgttcgta tttcaaaacc gaagctctac gggcaatctt
     3181 ccttcagcgg gggcatttcc tcggagt
//
LOCUS       DAOSWW010000210         3188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22102_length_3188_cov_3.102777, whole genome shotgun sequence.
ACCESSION   DAOSWW010000210 DAOSWW010000000
VERSION     DAOSWW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22102_length_3188_cov_3.102777"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PK442_05380"
     CDS             <1..709
                     /locus_tag="PK442_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO87114.1"
                     /translation="FIPWWGYILAVGLLSWYSSKKFVASIVLAALPFLIGMFGLWAMA
                     LESLSIIITAVLMALIIGLPVGVLMAEVRIIATILRPILDGMQTMPSFVYLIPAMMLF
                     GLGRVPAVLATLIYALPPVIRLTALGLNQVPKPVEEAALAYGATRWQLIKEVRLPLAM
                     PSILAGVNQTTMMALAMVVISSMIGARGLGQEVLLSINRIDIGRGFEAGISVVFLAIV
                     IDRLTQNMAKRWEPPKR"
     gene            716..2458
                     /locus_tag="PK442_05385"
     CDS             716..2458
                     /locus_tag="PK442_05385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/carnitine O-acyltransferase"
                     /protein_id="HOO87115.1"
                     /translation="MTEKKEKPAGTFEFQGSLPRLPVPALEETLGKFLLWTAPLLDER
                     GQKETREAVDAFLAPDGAGKTLQRRLEKWARETPESWLAGFWLRTYLDSDSPLPINSN
                     VFSLLDLPPVTGSSPRARRAAVLIAAALSLKKSIDDETLPPDTERGTPLCMSQYKNMF
                     GSTRIPGISGDELFVAPNSRHIAVFYRGRAWSLEVLSPEGEGASYDSIVARLDEISAS
                     VQQGEGIGVGTTLPRRHWGALREKLFSFCPENRRNMEMIESALFVLSLDPKNPEPLRE
                     KGFHYLAGTGANRWYDKSFQILVDEQGETGLNFEHSALDGSQHARLAAAILKTGERAS
                     AGEWKAPPALPLSFLLSEELAHELKEAERSFERLRENTRLRVLEFTSFGREKIKRLGM
                     SPDAFCQTAMQATIFDLYGKFLNAYEAVMTRQFLLGRTEAMRPVTPEIAAFVHARTAE
                     HLKAASSAHTGRIAECREGRGVDRHLFGLKKMMELFGQEIGVEETPALFSSPGYLALS
                     RNLLSTSSGPTECFRIYGFGPVDDEGFGVRYLMFPDKLVFTMTSRTVMEHRLLAFRER
                     LSENLEKKAALLSE"
     gene            complement(2551..>3188)
                     /locus_tag="PK442_05390"
     CDS             complement(2551..>3188)
                     /locus_tag="PK442_05390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO87116.1"
                     /translation="VALADPEMTATMPKNLTAFTGMDALVHAIEAYTCRVATPFSDAP
                     AITAMELIGKYLRRAWKDGGDLEAREGMLLGSLLAGMAFSHSDVAAVHCLAEALGAIY
                     DAPHGACNSIALPAIMEFSMDACVERYARVATCLGLSFSTPEEGAKKAVEEVKQMARD
                     LELPSISVFGVKEEDFEEIAAKSEANSSNPDNPKILSKADYVHILRKLTTA"
BASE COUNT          618 a          943 c          940 g          687 t
ORIGIN      
        1 ttttattccc tggtgggggt acatccttgc ggtaggcttg ctttcatggt attcgtcgaa
       61 gaaattcgtc gcatccatcg ttttggcggc gctccccttt ctcatcggga tgttcggcct
      121 ttgggccatg gcgcttgaga gtctctccat catcatcacc gccgtgctga tggcgttgat
      181 catcggtctt ccggtcggcg ttctcatggc ggaggtgcgc atcatcgcga cgattctccg
      241 gccgattctc gacggaatgc agaccatgcc gagctttgtc tacctcattc cggcgatgat
      301 gctcttcggt ctcgggaggg ttcccgcagt cctcgcaacg cttatctacg cgctgccccc
      361 ggtgatccgc cttacggctc ttggtctcaa ccaggtgccc aaaccggtcg aggaagccgc
      421 gctggcctac ggggcaactc gctggcagct gatcaaagaa gtccggcttc cgctcgccat
      481 gccgagcatt ctcgccggcg tgaaccagac gacgatgatg gcgctcgcca tggtggtcat
      541 ctcgtcgatg atcggcgcgc gggggctggg acaggaagtg ctgctttcga tcaaccgcat
      601 cgacatcgga cgcggtttcg aggcgggaat aagcgtggtc ttcctcgcca tcgtcatcga
      661 ccggctcacc cagaacatgg cgaagcgatg ggaaccaccc aagaggtgat cgtacatgac
      721 ggagaaaaaa gagaaacccg ccggcacgtt tgaattccag gggagccttc caaggctccc
      781 cgttcccgcg ttggaagaaa ctctcggcaa atttctgctc tggacggccc ctctgctcga
      841 cgaacgaggt caaaaagaga cgagagaggc tgtcgacgcg tttttggcgc cggacggcgc
      901 aggaaagacg ctccagcgac gcctcgaaaa atgggcccgg gagactcccg agagctggct
      961 cgcaggtttt tggctgcgaa cctaccttga ttcagattcc cccctgccga tcaacagcaa
     1021 tgttttttca ctgctcgatc ttccgcccgt aaccggttcg tcgccgcgtg cccggcgggc
     1081 tgctgttttg atcgcggcgg ctctctcgtt gaagaagagc atcgacgacg agacgctccc
     1141 cccggatacg gagcggggaa cgcctctctg catgagccag tacaagaaca tgttcggcag
     1201 cacgcgtatt cccggcatct caggagacga actcttcgtc gcgccgaaca gccgccacat
     1261 cgcagtcttt tacaggggga gagcatggtc gcttgaggtt ctctcgcctg agggggaagg
     1321 cgcttcgtac gacagcattg tcgcccgact cgacgaaatc tcggcatcgg tgcagcaggg
     1381 agagggtatt ggcgttggta cgactctgcc ccgcaggcac tggggagcgc ttcgggagaa
     1441 actcttctca ttctgcccgg agaaccgcag aaacatggag atgatcgagt ccgccctttt
     1501 tgttctctct cttgatccga aaaaccccga gccgcttcgc gagaaggggt tccattatct
     1561 cgcgggaacc ggagcaaacc gctggtatga caagtcgttc cagatactcg ttgatgaaca
     1621 gggagagacc ggcttgaact tcgagcactc cgccctggac ggttcccagc acgcacggct
     1681 tgctgcggct attctcaaaa cgggagagag ggcgagcgcg ggggaatgga aggctcctcc
     1741 ggctcttccg ctctcctttc ttctttcgga agaactcgct catgaactga aagaggctga
     1801 gcgctcattt gaacgtctgc gtgaaaacac aaggcttcgc gtgctggaat tcacatcctt
     1861 cgggcgcgag aagatcaaac gtctcggcat gagtccggac gctttttgtc agacggcgat
     1921 gcaggcgacg atcttcgatc tgtacgggaa attcctcaac gcgtatgaag cggttatgac
     1981 aaggcagttc cttctgggcc gcaccgaggc catgcgtccc gtcacgccgg agatagcggc
     2041 ttttgtgcat gcacgaacgg cggaacactt gaaggcggct tcctcggcgc acaccgggcg
     2101 aattgccgaa tgcagggagg gacgcggcgt cgaccgccac ctcttcgggc ttaaaaagat
     2161 gatggaactc ttcggacagg agatcggggt ggaagaaacg cctgcccttt tctcttcccc
     2221 ggggtatctc gcgctctcga gaaatctgct ctccacgagc tccggtccta cggagtgctt
     2281 ccgcatctac ggtttcggac ctgttgacga cgaggggttc ggcgtcaggt acctgatgtt
     2341 ccccgataaa ctggtcttta caatgacaag ccgtacggta atggaacacc gtctgctcgc
     2401 cttcagggag aggctgtcgg agaaccttga aaagaaggcc gctctcctct ccgaataaag
     2461 aggagcgaag gaggcggtcc tcccgctcac ccttcgctcc tctttttctc catttatttc
     2521 acctttttct catacattcc gcggtaactg ctatgccgtt gtcagcttcc tgagtatatg
     2581 gacgtaatcg gctttcgaga gaatcttcgg gttgtcgggg tttgacgagt tcgcctccga
     2641 cttcgccgca atctcctcga agtcctcttc cttgacgccg aagacggaga tcgaaggcag
     2701 ttcaagatcg cgcgccatct gcttgacctc ctccaccgcc ttcttcgcac cctcctccgg
     2761 cgtcgagaag gagagtccga ggcaggtggc gacgcgggcg tagcgttcca cgcacgcatc
     2821 catggagaac tccatgatcg cgggcagagc gatcgagttg catgcgccgt gcggcgcgtc
     2881 gtatatagcc ccgagagctt cagcaaggca gtggacggct gccacgtcgg agtggctgaa
     2941 ggccattccc gcaagaaggc tccccaggag cattccctca cgcgcttcca aatcgccgcc
     3001 gtctttccaa gccctccgca ggtatttgcc gatgagttcc atcgccgtga tcgccggggc
     3061 gtcggaaaaa ggcgtggcga cgcggcaggt gtaggcctct attgcgtgaa cgagagcgtc
     3121 catgccggta aatgcggtca ggttcttcgg catggtggcc gtcatctcgg ggtcggcgag
     3181 cgcaacct
//
LOCUS       DAOSWW010000211         3183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22153_length_3183_cov_3.789322, whole genome shotgun sequence.
ACCESSION   DAOSWW010000211 DAOSWW010000000
VERSION     DAOSWW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22153_length_3183_cov_3.789322"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PK442_05395"
     CDS             <1..811
                     /locus_tag="PK442_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOO87117.1"
                     /translation="LTPHIGANTEEAQSAVAAIAASNLAAALLGKPYEHAVNLPFSEQ
                     LLSDGSRAYLSLARKMGYLAANLLREPVREIKVALRGPLFTCGDEPVCFEVPYHYSPF
                     TVAGLKGMLEFSHGPEVNYMSAPLLAEDKGIAVEETRADGGTYRNLLELSITSSGGRE
                     TVTVCGTVTEEGRQRVVKLDGYPIEFVPEGVVLLFSNHDRPGVIGKVGTLLGEAGSNI
                     ANFALGRKNGSGLAVAALQIDNDVPDKVVETFKADADLLWAVKVDFSEAAR"
     gene            808..1578
                     /locus_tag="PK442_05400"
     CDS             808..1578
                     /locus_tag="PK442_05400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO87118.1"
                     /translation="MSCGHDHEKEEMNGMKPENSGERSPEVKEAAGTSPLRPTGAAGK
                     RIFLVRHGETSWNREGRFQGQIDVPLNDAGLRQADAVANTLKDVPFDRIISSNLSRAL
                     RTALPLASRMRLGMGVETDDDLKEIAHGLWEGRTAGEVEAMYPGALALWHTRPETVVM
                     PEGESLADVQRRAWPALLRIAEGKGETVAVFTHDAVLKVLLMKALDCPLSSFWRFQLA
                     NGSITLLELGNKGWRIPLMGEAGHLGSVFGKRAEQKGL"
     gene            complement(1694..2356)
                     /locus_tag="PK442_05405"
     CDS             complement(1694..2356)
                     /locus_tag="PK442_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HOO87119.1"
                     /translation="MFNQVDDLLEYAQGKTYRTILADPPWQFSNRTGKMAPEHKRLNR
                     YPTMPLGEICSLPVKEIAADTAHLYLWVPNALLPDGLTVLSAWGFEYKTNLIWYKTRK
                     DGGPDRRGVGFYFRNVTEMILFGVKGKNARTLQPGRSQENIITAQKREHSRKPDEQYR
                     LIESCSEGPYLELFARGPRPGWDVWGNQSEEYAPDWATYANHSQAKRDNPQQLTLIET
                     QA"
     gene            complement(2349..2945)
                     /locus_tag="PK442_05410"
     CDS             complement(2349..2945)
                     /locus_tag="PK442_05410"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HOO87120.1"
                     /translation="MPIELIPLNIRENYEVHEWKHAVAILSQDFPDEWTDILAVLESF
                     RLKKSWITDPGGRKSKVSEHIDSFLYRRGWVEKEFQTKVVVDDLSMSSPTHKVDCFKN
                     KVALEIEWNNKDPFYDRDLNNFRLLFDLRAISAGVIITRCDDLQDIFNDLGRGPSYGA
                     STTHMRKLLPKIEGGSGGGCPVLVFGITKHLYEEDDHV"
BASE COUNT          581 a         1033 c          920 g          649 t
ORIGIN      
        1 cctcacgccg catatcggcg cgaacaccga ggaggctcag tccgccgtcg ccgcgatcgc
       61 cgcgtcgaac ctcgccgccg cgctgctcgg gaagccgtac gagcacgccg tcaacctccc
      121 cttcagcgaa cagctcctct ccgacggcag ccgcgcctac ctctccctcg cccgcaagat
      181 ggggtacctc gccgcgaacc tcctgcgcga gcccgtccgc gagatcaaag tcgccctccg
      241 cggaccgctc ttcacctgcg gcgacgaacc cgtctgcttc gaagtcccct accactactc
      301 ccccttcacc gtcgccggac tcaaggggat gctggagttc tcgcacggcc ccgaggtgaa
      361 ctacatgtcc gcgccccttc ttgccgagga caagggcatc gccgtcgagg agacccgcgc
      421 cgacggcggt acctaccgca acctgctgga gctctccatc acttcctccg gagggcgcga
      481 gaccgtcacc gtctgcggca ccgtcaccga agaggggcgg cagcgcgtcg tcaagctcga
      541 cggctacccg atcgagttcg tccccgaagg agtggtgctg ctcttcagca accacgaccg
      601 ccccggcgtc atcggcaagg tcggcaccct gctcggcgag gccggatcga acatcgcgaa
      661 cttcgccctc ggccggaaga acggaagcgg cctcgccgtc gccgcgctcc agatcgacaa
      721 cgacgtcccc gacaaagtcg tcgagacgtt caaggccgac gccgacctgc tctgggccgt
      781 caaggtcgac ttttcggagg cggcgcgatg agctgcgggc atgatcatga gaaggaggag
      841 atgaacggta tgaagccgga aaattcaggg gagcgttccc cggaggtgaa agaggctgcg
      901 gggacgagtc ccctgcgacc aacgggagct gcggggaagc gcatcttcct cgtccggcac
      961 ggcgagacgt cgtggaaccg cgaagggcgc ttccaggggc agatcgacgt cccgctcaac
     1021 gacgccggac tgcgccaggc cgacgccgtc gccaacaccc tgaaagacgt cccattcgac
     1081 cggataatct ccagcaacct ctcccgcgcg ctccgcaccg ccctccccct cgcctcccgc
     1141 atgcgcctcg ggatgggcgt cgaaaccgac gacgacctca aggagatcgc ccacggcctc
     1201 tgggaggggc gcaccgccgg cgaagtggag gcgatgtacc ccggagccct cgccctctgg
     1261 cacacccgcc ccgagaccgt cgtcatgccc gaaggcgaga gcctcgccga tgtgcaacgc
     1321 cgagcctggc ccgccctgct gcgcattgcc gagggaaagg gcgaaaccgt cgccgtcttc
     1381 acccacgacg ccgtgctcaa agtcctgctg atgaaggcgc tcgactgccc cctctccagc
     1441 ttctggcgct tccagctcgc caacggcagc atcaccctgc tggagctggg aaacaagggg
     1501 tggcgcatcc cgctgatggg cgaggcgggg cacttgggaa gcgtcttcgg gaagagggcg
     1561 gagcagaagg ggttgtaaca aatcagaccg atgaacgtct tttccgacag taggctccac
     1621 cggacccaaa ccttcgcctc gaatatgagc aaaactccct gctgcgtctc cgaggcgaag
     1681 gttcagtatt gcatcacgcc tgtgtttcga tcaaggtgag ctgctgcggg ttgtctcgct
     1741 tggcctggga gtggttcgcg taggtcgccc agtcgggggc gtactcctct gactggtttc
     1801 cccacacgtc ccatccgggg cgcggaccgc gcgcgaagag ttccagatac ggcccttcgc
     1861 tgcacgattc gatcaggcgg tactgctcgt ccggctttct gctgtgctct cttttctgcg
     1921 ccgtgatgat gttctcctga gagcgtcccg gctggagggt ccgggcgttc ttgcccttca
     1981 ctccgaagag gatcatctcg gtgacgttcc ggaagtaaaa gccgactccg cggcggtcgg
     2041 ggcctccgtc cttccgcgtt ttgtaccata tcagattcgt cttgtattcg aagccccatg
     2101 ccgaaagcac cgtcagcccg tcgggaagga gcgcgttggg aacccataag tagagatgcg
     2161 ccgtatcggc ggcgatctct ttcaccggaa ggctgcaaat ctcgccgagc ggcatcgtcg
     2221 gatagcggtt gagccgttta tgttcgggcg ccatcttgcc ggtgcggttc gagaactgcc
     2281 agggaggatc ggccaggatt gtcctgtatg tcttcccctg cgcatattcc agcagatcgt
     2341 ctacttgatt aaacatgatc gtcttcctcg tagaggtgtt tggttatgcc gaacaccagc
     2401 acaggacatc cgccgccgct tccgccttcg attttgggta gcagctttct catgtgggtg
     2461 gtcgacgcgc cgtaggaggg gcctcgtccg agatcgttga aaatatcctg caggtcgtcg
     2521 catcgggtga tgataactcc cgcgctgatt gcccgcaggt cgaaaagcag ccggaaattg
     2581 ttcaaatcac ggtcgtagaa ggggtctttg ttgttccatt cgatctccag tgccactttg
     2641 tttttgaaac agtcgacttt atgcgtgggc gacgacatcg acaagtcgtc cacgaccact
     2701 ttggtctgga actccttttc aacccagccg cgcctgtaga gaaagctgtc gatgtgttca
     2761 gacaccttcg attttcgccc gcccggatcc gtaatccatg attttttcaa acgaaaggat
     2821 tccaggacgg ccagaatatc cgtccactcg tcggggaaat cctgcgaaag gatggcgacg
     2881 gcgtgcttcc actcgtgaac ttcgtaattt tctctgatgt tgagcggtat caactctatc
     2941 ggcatgtttt cacctcttcc gcatcgtacc atattttcac gaatttgtgg cgcctgtcgt
     3001 gggggaaata cgtccgttgc gctctgcgta caatcagcct ttttctctca atcgacgttt
     3061 ttcctaaaat tttcttcggt ggacgtcctg catcaccctc cgagaatcgc gtcgatgaac
     3121 tctgcttttt tcttcagccc cttgccggga attcccttct tcttcatgat ctctttgagt
     3181 acg
//
LOCUS       DAOSWW010000212         3174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22242_length_3174_cov_4.051298, whole genome shotgun sequence.
ACCESSION   DAOSWW010000212 DAOSWW010000000
VERSION     DAOSWW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22242_length_3174_cov_4.051298"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PK442_05415"
     CDS             <1..360
                     /locus_tag="PK442_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO87121.1"
                     /translation="GEGIYSLAGKLSGGNQQKVCIARAITLAPNLLFVSEPTRGIDIG
                     AKKLILDHLVKLNRENGLTIIMTSSELAELRSICDRIAIVYQGKLEGILLPADSDRDF
                     GLMMAGEYHKFHEKEAV"
     gene            362..1402
                     /locus_tag="PK442_05420"
     CDS             362..1402
                     /locus_tag="PK442_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87122.1"
                     /translation="MGNSLLNRIGIPRLIISLFLAFLVASAFSYGLPMGQIFGAMLTR
                     FGMNALLVLAMIPTIQAGAGPNFGLPFGIICGLIGATLAIELDLRDFTALFFAIGVSV
                     PLGAFAGWLYGLLLNRVKGQEMTVGTYMGFSIVSLMCIFWLMAPYTSPEMVWPYGGDG
                     LRVTVVLDGRMEQLLDKLWSFTIGTVRIPTGLLVVTAICCVLLWIFMKTRTGLAMSIV
                     GSNPRFAEASGLNVNKYRLIASVISCAMGAAGIVIYSQSYGFIQLYQAPLYMALYSVS
                     AILIGGASLQRATITQALIGTFLFNALLVIALPVANVAMDSDISEIMRVIISNGIILY
                     ALTRKEVGGDAR"
     gene            1399..2472
                     /locus_tag="PK442_05425"
     CDS             1399..2472
                     /locus_tag="PK442_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87123.1"
                     /translation="MKLIQKNIVPIVFIILCTIGFQYSGLSWIFFANDLMTRLARNSF
                     LVISLIIPVLAGMGLNFGIVLGAMAGQFAAFITVVHNLTGIGGFFAACVMAIPFAILF
                     GWLTGILFNKAKGKEMITGLILGFFANGIYQLVCLIFIGWLIPISNKALLLPSGVGFV
                     NTLDLKIWQYAIDKFYVFRARGIDIPVLKYLNNINIPVLTLTIVVLLCIAVVLLFRTK
                     LGQDFRAVGQNRHIAEIAGIKVDRVRIIAVIFSTVLAAWGQLIFLQNIGNVQVYGSHV
                     QVGTFAVAALLIGGATVSKATIGQALLGTVLFHALFIVSPLAGKNLMGSAQVGEYFRV
                     FIAYGVIGIALALHAWQRLVKKQ"
     gene            2687..>3174
                     /locus_tag="PK442_05430"
     CDS             2687..>3174
                     /locus_tag="PK442_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO87124.1"
                     /translation="MSENGFACYSLREELLLALNKKGFSIPTPVQEKVLSMDRFDTDL
                     IVRAKTGSGKTLAFLLPLLQEMELPASAPLILVLSPTRELAQQTAREAEWLSRYLDIS
                     VATLVGGLDMTTQIRSLKEGAVVVVGTPGRTLDHVNRGSLKTEKIHTVILDEGDHMLD
                     MG"
BASE COUNT          660 a          887 c          823 g          804 t
ORIGIN      
        1 ggtgaaggca tttactccct tgcagggaag ctctctggag gcaatcagca aaaagtctgc
       61 atcgcccgcg ccatcacgct tgcgccgaat cttctcttcg tctccgaacc tacgcggggt
      121 atcgacatcg gcgccaagaa gctcatcctc gatcatcttg tcaaactgaa cagagagaac
      181 gggcttacta taatcatgac ctcttctgag ttggcggagc ttcgctccat ctgcgaccgg
      241 atcgcaatcg tctaccaggg gaaactggaa ggcatactgc ttcccgcgga cagcgaccgc
      301 gattttgggc ttatgatggc aggagagtat cataagttcc atgaaaagga ggctgtctga
      361 tatggggaac agtcttctga accgcatcgg gattccacgc ctcatcatct cccttttcct
      421 cgcatttctt gtcgcttcgg ctttcagtta cggccttccc atgggacaga tcttcggagc
      481 tatgcttacg cgtttcggga tgaacgcact tttagtgctc gcaatgattc cgaccattca
      541 ggccggagct gggccgaatt tcggccttcc gttcggtatc atctgcggac ttatcggcgc
      601 cacgctcgct atcgaactcg atctgcgaga cttcacagcg ctcttcttcg cgatcggggt
      661 ctccgtacct ttgggggcat tcgcgggctg gctttacggc ttgctgctca acagggtcaa
      721 aggacaggag atgacagtcg gcacctatat gggattctct attgtgtcgc tgatgtgcat
      781 cttctggctc atggctccct atacaagccc tgaaatggtc tggccctatg gtggagatgg
      841 tctccgggtc accgttgtgc tcgatggcag gatggagcaa ctcctggaca agctgtggag
      901 cttcacaata ggcacagtac ggattcctac cggcttgctt gttgtgacgg cgatctgctg
      961 cgttcttctc tggatcttta tgaaaacacg tacgggtctt gccatgtcca tcgtcggctc
     1021 caaccctcgc ttcgcggaag cgtcggggct gaacgtcaat aaataccgtc tcatcgcttc
     1081 ggtcatttcg tgtgcaatgg gcgccgccgg tattgtcatc tactcgcaga gctacggttt
     1141 catccagctc taccaggccc ccctctacat ggcgctctat tcggtatccg ccattctcat
     1201 cggcggcgct tcgctgcagc gggcgacgat cacgcaagcg ctcataggca cattcctctt
     1261 caatgcgctc ctggtcatcg cgctcccggt agccaatgta gccatggaca gcgacatctc
     1321 ggagatcatg cgggtcatca taagcaacgg catcattttg tacgcgctca cccgcaagga
     1381 agttggaggt gacgccagat gaagctcata cagaaaaaca tcgttccgat agtgttcatt
     1441 atcctctgca ccataggatt ccagtactcg gggctctctt ggatattctt cgccaacgac
     1501 ctcatgacgc ggctcgcccg gaactccttc ctggtgatct ccttgatcat tcccgtcctt
     1561 gcgggcatgg gattgaattt cggaatcgtc ctcggagcga tggccggaca gtttgcggcg
     1621 ttcatcacgg tcgtgcataa cctcacgggg atcggcggtt tcttcgcggc gtgcgtgatg
     1681 gcgattccat tcgccattct cttcggctgg ctgacgggca tcctcttcaa caaggccaaa
     1741 gggaaagaga tgatcaccgg actgattctt ggcttctttg ccaacggtat ctaccagctg
     1801 gtctgcctga tctttatcgg atggctgatt cccatctcga acaaggcgct tctcctgcct
     1861 tccggtgtcg gttttgtgaa cacgctggac ctgaagatat ggcagtacgc gatcgacaag
     1921 ttctacgtct tccgcgcccg cggcatagac ataccggtgc tcaaatatct gaacaacatc
     1981 aacattccgg tattgaccct gacgatagtt gttctcctgt gcatcgccgt cgtccttctt
     2041 ttcaggacaa agcttgggca ggacttccgg gctgtgggac agaacaggca tatagcggaa
     2101 atcgcgggaa tcaaggttga cagggtacgt attattgccg tcatcttttc cactgttctg
     2161 gctgcatggg gacagctcat cttcctccag aacatcggaa acgtgcaggt ttacggttct
     2221 catgtgcagg tcgggacatt tgcggttgcg gcgcttctca tcggaggcgc aaccgtctcg
     2281 aaagccacta taggtcaggc gcttcttgga accgtactct ttcacgccct tttcatcgtc
     2341 tccccacttg ccgggaaaaa tcttatggga agcgcacagg taggcgaata cttccgtgtt
     2401 ttcattgcct acggcgtcat tggcatcgct ctggcgcttc acgcctggca acgattggta
     2461 aaaaaacagt aagattctcg tcacacagat tcgcgcctcc atattgacat attctgaaat
     2521 tcatgtataa tttcagtttg tcaatggagg gaagaatttg agtggacccc cggaacgggg
     2581 tccactcttt tttggggaac gcgggccgaa cacagaacgg caagtactat ttgatgcatt
     2641 attccccagg atttgccctc ctaatttgag gaggaataat atactcatga gtgaaaacgg
     2701 tttcgcgtgc tattctctgc gggaagaact tcttctcgcg ctcaacaaga aaggcttttc
     2761 aatccccaca ccggtccagg aaaaagtgct ttcgatggac cgtttcgata ccgacctgat
     2821 cgtccgggca aagacgggat cgggcaaaac gctggcgttc ctgctccctc tgctccaaga
     2881 aatggagctt cccgcatcag cccctctcat cctggtgctt tcacccaccc gtgagcttgc
     2941 acagcaaaca gctcgtgagg cggagtggct ttcacgttac ctcgatattt ccgtcgccac
     3001 tctcgtcggc gggctggata tgacgactca aatccgttcg ctcaaagaag gcgcagtcgt
     3061 cgtcgtcggg actcccggaa gaacgcttga tcatgtcaac cgcggttcgc tgaagacgga
     3121 gaaaatccac actgtcatcc tggacgaggg cgatcacatg ctcgacatgg gatt
//
LOCUS       DAOSWW010000213         3158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22412_length_3158_cov_4.014502, whole genome shotgun sequence.
ACCESSION   DAOSWW010000213 DAOSWW010000000
VERSION     DAOSWW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22412_length_3158_cov_4.014502"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..167
                     /locus_tag="PK442_05435"
     CDS             15..167
                     /locus_tag="PK442_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO87125.1"
                     /translation="MCKKCGICAKNCPVHCIPGDRTSGYRIETDKCIRCGTCFEKCPF
                     GAIEKK"
     gene            187..2202
                     /locus_tag="PK442_05440"
     CDS             187..2202
                     /locus_tag="PK442_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO87126.1"
                     /translation="MSEITLVIDGVECRASKGETILQVARKNNIYIPTLCWMEGLTPI
                     GSCRMCVVEVAGNPKLLTACTTPAENNMQVSTKTEKLGKYRRQILELLFAGRNHFCMF
                     CSQTGDCELQRLAIEHGMDNVRYPFLYETFHNDASDPNIQIDHNRCILCLRCIRVCAE
                     KVGAHTLDLEKRGWGTNIMADFGRQLGESDSCVSCGACAQVCPTGTITLRDFVYRGRR
                     HDCDDQVESICPLCSLGCKIKAYVRTGSVVRVEGISGDSPDGGQLCHMGRWWLPKSTE
                     RERLSVPMIRTGSSFREASWEEALDLIAERMAPALKADRAGALVSELSTDEELTVVSS
                     FFRDTLEMKKMDGFYGNMLRGFVRGLKPFADQGVRPFTGAHNILASDAIVVVEANPQE
                     EAPVAASYIRVSTVIERAKLINVSSGKNPFDGISDQDLRTTEKNMLSLLTALRKAVEG
                     DEDGAAPEVKEIASILRNAKKPVVVIGSKLAEENPLLVTEAVNLAVASKAFFEDGLGV
                     VPMLRAGNSLGALNTITGESDWVSDGHLDFLYVLSTGMVAEDKRSLEAMTRARFVVVH
                     TPFMVHPLVNMADVLLPAPAWFERSGHYCTLEGERRRMNLVTAPTGNIKGLSVIMKEL
                     AGRLGVEMQKASAAPCENVYEAKAAPDTARTVRFAPGASVPFVKEVR"
     gene            2205..2744
                     /locus_tag="PK442_05445"
     CDS             2205..2744
                     /locus_tag="PK442_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOO87127.1"
                     /translation="MAKPKIASVWLEACAGCHMSFLDIDERIVDLLDKVELTATPITD
                     IKEFPEVEVGVIEGALGNEEELHLAKELRERCKILIAWGDCAVFGGINCMRNFMPAEQ
                     VLREGYVETVSTVNPEGVIPGKDLPALLPYAIPVDRAVKVDVYVPGCPPDADTIYYVF
                     SEILEGRIPSVPTDIMRYD"
     gene            2759..>3158
                     /locus_tag="PK442_05450"
     CDS             2759..>3158
                     /locus_tag="PK442_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOO87128.1"
                     /translation="MENKKNTIVINPITRIEGHGKITVDLTDDGAVDNVRFHVTQYRG
                     FEVFSKGRDFREMPVITPRICGICPVSHHLASAKACDAILGVTPPAPAAKLREVMHMG
                     QFIQSHALSFFHLSSPDLLFGFDADPAVRNV"
BASE COUNT          681 a          893 c          959 g          625 t
ORIGIN      
        1 ccatacttca ggacgtgtgc aaaaagtgcg gtatatgcgc aaagaattgc cctgtgcact
       61 gcatccccgg cgaccggact tctgggtacc gtattgagac cgacaagtgt atccgctgcg
      121 ggacgtgctt cgagaagtgc cccttcggcg ccatcgagaa aaagtagtcg tggaggagga
      181 aaactcatga gtgaaatcac cctcgtcatc gacggagtgg agtgcagggc gtcgaaagga
      241 gagactatcc tccaggttgc ccggaagaac aatatatata tcccgaccct ttgctggatg
      301 gagggactga ctcctatcgg ttcctgcaga atgtgcgtcg tcgaggtggc gggcaacccc
      361 aagctcctta ccgcgtgcac aacgccggcc gagaacaaca tgcaggtttc gacgaagacc
      421 gaaaagcttg gaaagtatcg acggcagata ctggagctgc tcttcgccgg gcggaaccac
      481 ttctgcatgt tctgctcgca gacgggagac tgcgagctgc agcgccttgc catcgaacac
      541 ggcatggaca atgtccgtta tccgttcctg tacgagacgt tccataacga cgcttcggac
      601 ccgaacatcc agatagacca caaccgttgt atcctctgtc ttcgctgcat cagggtctgc
      661 gccgagaagg tcggcgcgca tacgctggac ctcgaaaagc gcggatgggg gaccaacatc
      721 atggcggact tcggcagaca gctcggcgag agcgattcct gcgtctcgtg cggcgcgtgc
      781 gcacaggtct gccccaccgg cacgatcaca cttcgcgatt ttgtctaccg cggccgtcgt
      841 catgactgcg acgaccaggt ggagagcatc tgtccgctct gttcgctcgg gtgtaagatc
      901 aaagcgtacg ttcgcacggg gagcgtcgtc cgggtagaag gtatctccgg cgacagtcct
      961 gacggcggac agctctgcca catggggcgc tggtggcttc cgaaatccac ggaacgcgag
     1021 cgtctttccg tccctatgat ccgtaccggt tcctccttcc gggaggcgtc gtgggaagaa
     1081 gcattggatc tgatcgctga aagaatggct cccgcgctga aggccgacag ggccggagcg
     1141 cttgtgagcg aactttcgac cgacgaagag ctgacggtgg tctcatcctt cttccgcgat
     1201 acgctggaga tgaaaaagat ggacggcttc tacggcaata tgctccgggg cttcgtaaga
     1261 gggctgaaac ccttcgccga ccagggagtc cgtccgttca caggcgctca taatattctg
     1321 gcgagcgacg ctatcgtcgt cgtcgaggcg aacccgcagg aagaagctcc ggttgctgca
     1381 agctatattc gtgtatcgac cgtcattgag cgagcgaagc tcatcaacgt ctccagcgga
     1441 aagaatccgt tcgatggcat ttctgatcag gatctcagaa caacggaaaa gaacatgctc
     1501 tctcttctta ccgctctgcg caaggccgtg gagggcgacg aggatggagc ggctccggag
     1561 gtcaaggaga ttgcgtccat ccttcggaat gcgaaaaaac ccgttgtggt catcggctcg
     1621 aagcttgccg aggagaatcc tctgcttgtt accgaggcag tgaacctggc tgtcgcctcg
     1681 aaggcgttct tcgaggacgg actcggagtc gtcccgatgt tgcgggccgg aaacagcctg
     1741 ggagcgttga acacaatcac gggggagagc gactgggtga gcgacggaca tctcgacttc
     1801 ctctatgtgc tctcgacggg gatggtcgcg gaggacaaac gttcgctgga agccatgacc
     1861 cgtgcgcgct tcgtcgtggt gcacacgccc ttcatggtgc atcctctggt gaacatggcg
     1921 gacgtgttgc tgccggctcc ggcatggttt gaacgaagcg gtcactactg caccctggaa
     1981 ggggaacgcc gcagaatgaa ccttgtgacc gcgcccaccg gcaatataaa agggctgtcg
     2041 gttattatga aggaactcgc cggaaggctc ggggtcgaaa tgcagaaggc ctccgccgct
     2101 ccctgcgaga atgtctacga ggcgaaggct gccccggaca cggcccgaac ggtccgcttc
     2161 gctccgggag cttctgtacc ctttgtgaag gaggtacgat gattatggcg aagccgaaaa
     2221 tcgcgtcagt atggcttgaa gcctgcgcgg ggtgccacat gtcctttctc gacatagacg
     2281 agcgcatcgt agaccttctg gacaaagtgg agcttaccgc cacgcctatt accgacatca
     2341 aggagtttcc ggaagtggag gtgggcgtta tagaaggagc gctcggcaac gaggaggagc
     2401 tgcacctggc gaaagagctc cgcgaacgct gcaaaattct catcgcatgg ggagactgcg
     2461 ccgttttcgg aggcattaac tgtatgcgca actttatgcc cgccgagcag gtgctcaggg
     2521 aaggatatgt ggagacggtc agcacggtga accccgaagg ggtcatcccc gggaaagacc
     2581 tccccgcact gctcccgtac gcaattcccg tggaccgggc ggtgaaggtg gacgtctacg
     2641 ttccggggtg ccctcccgac gccgacacca tctattatgt cttcagcgag attctggaag
     2701 ggcggattcc ctccgtcccg acggacatca tgcgctacga ttagcggagg accgaagaat
     2761 ggagaacaag aaaaatacga tagtgatcaa tcccattacc cgcatcgagg gccatggcaa
     2821 aatcaccgtg gacctcacgg acgacggagc ggtggacaat gtccgcttcc acgtgacgca
     2881 gtacagaggg ttcgaagttt tttcaaaggg tcgcgatttc cgcgagatgc ccgttatcac
     2941 gccgcgcatc tgcggtatct gcccggtgag ccaccatctt gcgtccgcaa aagcatgcga
     3001 cgcaatcctc ggcgtgacgc ctcccgctcc ggcggcgaag ctccgcgagg tgatgcacat
     3061 ggggcagttc atccagtccc atgcgctctc cttcttccac ctctcgagtc ccgatctcct
     3121 tttcgggttc gatgccgacc cggctgtacg aaatgtag
//
LOCUS       DAOSWW010000214         3158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22417_length_3158_cov_2.892362, whole genome shotgun sequence.
ACCESSION   DAOSWW010000214 DAOSWW010000000
VERSION     DAOSWW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22417_length_3158_cov_2.892362"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK442_05455"
     CDS             complement(<1..268)
                     /locus_tag="PK442_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87129.1"
                     /translation="MSKSEKWVFSLLFLSLVLIHPLAAAAAPGNEIALTVDDEQVSVD
                     EILYLLALQSGAGEAAAPLLAAQMTPTEREAFLEQVSRAILFSKG"
     gene            complement(286..1113)
                     /locus_tag="PK442_05460"
     CDS             complement(286..1113)
                     /locus_tag="PK442_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOO87130.1"
                     /translation="MDGSSLWFYFLLFFVVFPYLKQQSLRWRRFTALKNCEKRRRSRI
                     ITLIHRQESMGFWGMFSRNFINIEDSEEVLRAIRLTSDDTPIDLIIHTPGGLLLAAEQ
                     IARALKHHPAKVTVIVPHYAMSGGTLIALAADEILMDRHAVLGPVDPQIGQYPAASIL
                     KAVEEKPVSEVDDNTLILADISRKAIRQTKNFVFELLSERMGEEKGMELAEALTEGRW
                     THDYPIGVDEATALGLPVVLNDYDSFCNIMGLYHQSVTERPSVQYIPVPYPGRPSGN"
     gene            complement(1153..>3158)
                     /locus_tag="PK442_05465"
     CDS             complement(1153..>3158)
                     /locus_tag="PK442_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOO87131.1"
                     /translation="TPTLDQLALDLSERSRNGELDPVIGRSKEIRRIVQILSRRTKNN
                     PVLIGEPGVGKTAIVEGLAQKIMEGDIPELLKDKKVMQLNMGNLVAGTKYRGEFEERM
                     RKLVKELSEGRDVILFIDEIHTIVGAGGAEGAVDAANILKPSLSRGEFQVIGATTLEE
                     YRKHIEKDAALERRFQPVFVQEPSVDDSIRILEGLRDRYESHHKARIQDDALEAAARL
                     SARYIMDRRLPDKGIDLIDEAAARARIKTMEPPEELKTLEQRLEEIRKEKEAAVDGQE
                     FERAAELRDAERLLFEKLEEKRKNWRSGRNHSQPVITGEDIADVVAEWTGIPVFQITG
                     EESLRLLGMEREIEKRLVGQSEAVASVARAIRRARSGLKDPKRPIGSFLLLGPTGVGK
                     TELARRLASFLFGTDDSMIRLDMSEFMEKHEVSKLIGAPPGYVGYEDGGKLTEAVRRR
                     PYSVVLFDEIEKAHPDIFNLLLQILEDGRLTDGHGRTVDFRNTVILMTSNVGARDAAK
                     GSPLGFAGSGNDDPFDWSRLKSTILDEVNRTFRPEFINRVDESIVFRPLGRESVGAIV
                     DIMFADVCSRLSGQEVTLTVGEEVKNLVCEKGYQPKFGARPLRRTIQALVEDPLADYL
                     LENGASKGLHVEAFVSEGHVTFSVVPAPVVESSEDRARETVHQ"
BASE COUNT          708 a          920 c          770 g          760 t
ORIGIN      
        1 cccccttcga gaagagaatc gcccgcgaaa cctgttcaag aaacgcctct ctctcggttg
       61 gagtcatctg cgcggcaagc agaggggcag cggcttcgcc ggcccctgac tgaagagcta
      121 aaagatagag tatttcatca acagaaactt gctcgtcatc aacagtaagg gcaatttcat
      181 tccccggcgc tgccgctgcg gcaagcggat gaattagtac gagagaaaga aaaaggagac
      241 tgaaaaccca cttttccgac ttgctcattc gaacacaccc gcctttcaat ttccggaagg
      301 gcgtcccggg taggggacgg gaatatattg gacggaaggc ctctcggtaa cggactgatg
      361 gtacaacccc atgatgttgc agaaggagtc ataatcattg agcacaaccg gaagcccgag
      421 agcggtcgct tcgtctactc ctatcgggta gtcatgagtc catcggcctt ccgtgagagc
      481 ctccgcgagc tccatcccct tctcttcacc cattctttcg gaaaggagct caaaaacgaa
      541 gttcttcgtc tggcggatcg cctttcggga gatatcggca agtatcagcg tattgtcatc
      601 aacctcgctg acgggttttt cttctactgc tttcagtata gaggccgccg ggtactgccc
      661 tatctgagga tccacaggac cgagaaccgc atggcgatcc atcaggattt cgtcggctgc
      721 gagagcgata agcgtcccgc ctgacatggc gtaatgcggg acgataacag taacctttgc
      781 cggatgatgc ttcaaagcgc gtgcaatctg ttccgctgcg agaaggagac cgcctggcgt
      841 atgtatgatc aggtctatgg gggtatcgtc cgaggtcaag cgaatagctc gaagaacctc
      901 ctcggagtcc tcaatattga taaagttccg ggagaacatc ccccagaatc ccatgctctc
      961 ctgtcggtga atcaaagtta taatccgtga acggcgtctc ttttcacagt ttttgagcgc
     1021 agtaaaacgc ctccaccgaa gcgactgctg ctttaagtaa ggaaagacta caaaaaagag
     1081 caggaagtaa aaccaaagcg atgatccgtc catgacagcc tccttccaga aacaacgagc
     1141 acaagcgaat aactactgat gcacagtttc acgcgcacga tcctccgaag actctacaac
     1201 gggcgcaggg acgacgctga aagtgacatg tccctcggaa acaaacgcct cgacgtgcaa
     1261 cccctttgaa gccccgtttt caagaagata atcagccaag gggtcttcga caagcgcttg
     1321 tatcgtcctt ctgagaggcc gggccccgaa ctttggctga taccctttct cgcacactaa
     1381 gttcttcact tcttctccca ctgtcagcgt gacctcctgg cccgacaatc gcgaacagac
     1441 gtctgcaaac ataatatcaa caattgcgcc gacgctctct cttcctaagg ggcgaaagac
     1501 aatactctcg tcgacacgat ttatgaattc gggacggaag gttcggttca cttcgtcaag
     1561 gatagtgctt ttaagacggc tccagtcgaa cggatcgtcg tttcccgatc cggcgaatcc
     1621 aagaggagac cccttcgcgg catctctggc gccgacgtta ctggtcatca ggattacggt
     1681 attgcggaaa tccactgttc gtccgtgccc gtcagtcaat cggccatctt ccagtatctg
     1741 gagaagcagg ttgaatatgt ccggatgcgc tttttcaatt tcatcgaaaa gcaccacgga
     1801 ataggggcgg cgccgcactg cttccgtgag tttcccccca tcttcgtacc caacgtaccc
     1861 gggaggagcg ccaataagct ttgagacttc atgtttctcc atgaactcgc tcatatcgag
     1921 acgaatcatt gaatcgtccg tcccgaagag gaaggatgcc aggcgtctcg ccagctcagt
     1981 cttgcccact cctgtaggcc caagcagaag aaagcttcca atgggccgct tcgggtcctt
     2041 cagaccgctt ctggcgcgcc tgatagccct cgcaaccgac gcgacagctt cgctctgtcc
     2101 tacgagcctc ttttctatct cgcgttccat tccaagaaga cgcaaagact cttcccctgt
     2161 gatctgaaac accgggatgc cggtccattc tgcaactacg tccgcaatat cttcacctgt
     2221 gatcactggc tgagaatgat ttctccccga cctccagttt ttccgtttct cttccagctt
     2281 ttcgaaaagc agacgctcgg cgtcgcgcag ttccgcagcc ctttcaaatt cctgcccgtc
     2341 cacggcggct tccttctctt tacgaatttc ttccagacgc tgttcgagcg tctttagttc
     2401 ttcggggggc tccatcgtct tgatcctcgc tctggcggcg gcttcgtcga taagatcgat
     2461 tcctttatcg ggaagacgtc tgtccataat atagcgagca gaaagccggg cggccgcttc
     2521 cagggcgtca tcctgtattc tggccttgtg gtgcgattca taccggtctc tgagtccctc
     2581 cagaatacgg atggagtcat ccacggacgg ctcctgaaca aataccggct ggaagcgtct
     2641 ttcgagcgca gcgtccttct cgatatgctt cctgtactct tccagggtag tggcgccgat
     2701 aacctggaat tcccccctgg aaagactcgg cttcaaaatg ttggccgcat ccaccgcgcc
     2761 ttctgcgccc ccggcgccga caatagtgtg gatttcgtcg atgaagagaa tgacgtcgcg
     2821 tccttcggag agttccttca ccagtttacg catgcgctcc tcgaactctc ccctgtattt
     2881 cgtgcctgca acaagattcc ccatgttgag ctgcataacc ttcttatcct tcagaagttc
     2941 ggggatatct ccctccataa ttttctgcgc cagtccctcg acaattgcgg tctttcctac
     3001 cccgggttct ccgataagca ccggattgtt ctttgttctt cgggagagga tctggacgat
     3061 tctacgaatt tctttgcttc ttccaataac cggatcgagt tcaccgtttc tgctccgctc
     3121 actgagatcc aacgcgagct ggtccagtgt aggcgtct
//
LOCUS       DAOSWW010000215         3157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22424_length_3157_cov_4.277885, whole genome shotgun sequence.
ACCESSION   DAOSWW010000215 DAOSWW010000000
VERSION     DAOSWW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22424_length_3157_cov_4.277885"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3157)
                     /locus_tag="PK442_05470"
     CDS             complement(<1..>3157)
                     /locus_tag="PK442_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87132.1"
                     /translation="ADILAKLGVFHDPLVSSIKEELKSVGKPSYAKVVGKEFDRMRKA
                     ARIVRSPMLKETERQRFESMHPQSLVTMERMYKEAKRRLDEAKAEPVLGLGLGERIDK
                     ASLSEQRHLVASGLQKILEVKASPEWRNRMRRVRAGEEDLFSSRVFDEVGLQENLAKS
                     LRPDITNWEAAGPVFAAGGWLLAKAAERTGDSWSAKDQKIRALVADVAAAAAVGDFAA
                     AHREVELERNLSRKDRFGLDRAIVRASLDYDVRRIGDNSRTDNDMTTGSLVGHWELIS
                     MYSAQRAAIGGVDYLKEEIPRLLGLVSAWENRLNMVIRAEGRKFGEGRERRTIVRMVK
                     NEKDSKKLGRDVYDYQDKIYNRAKDNLRELTDAELNEQELLGYLVMAIIEGKEYLTFG
                     TDSYRGAKTELGEDGEERPSEYVWVFNGEVVAEDRAGAKRIRQVDGWDAKEHRPTSAF
                     MSVDLAIKKLMESDYFKTLQEYGRPLSMIDIRRGENGSLEGGLVEYAKTIQKELRDAM
                     ADYRDIEAWTGRAASEGVVGEIQRYLPHVFGGHGFEAAGRRKAREAVHRQVRESAGPR
                     AGGQRRLPPAALKASFKDVGTDKEAVPEQEALYVQLSDELDHLYRKKKKKAPRMALAT
                     EKERKDKDSPWSKIMYNRALLRLATRVETERAELVGAEHYKIGDPTQGGTEGTAELIR
                     GLQQSIRDYSEREWTARRGKREYPSYYDAFIQSTNDKEKVSALMPAISDPLRLAETSI
                     KDMMDTLTREVLVNQFMLAEDAAQNPFVIAIPREGVKDTDPANRGRDIRSMLREDTLR
                     KQLRMLERKAGRAGRGDTLHEKLRSLVSGNERAVNLDNYELVKSDYESIQAFYVLKKE
                     AGYDSRARYWFQHVVGRPAQWNVEIGEEFEHDVLKTLSKVNMFMKHMAVAWSLFFATA
                     GVESTVAATPLNRNMANIFQKQFWENWASVARLGRRVRRGDLEIAMDLYKYHKAGIMI
                     SERNISDGSSSVVDEVISKVSRSLGERFGGEDDAKRAKYEGRARTALRTLTGRKLSEW
                     LMGDFFGGLKLWSTQFLIDEF"
BASE COUNT          516 a         1059 c          811 g          771 t
ORIGIN      
        1 tggaactcgt ctataagaaa ttgcgtcgac cacaatttca gtccgccgaa gaagtccccc
       61 atcaaccact cagacagttt gcgtccggtc agcgtgcgca gcgcggtccg cgcccggccc
      121 tcgtacttgg ctcttttggc gtcgtcttct cctccgaacc gctctccaag cgagcgggac
      181 actttggata ttacttcgtc caccaccgag ctggagccgt cagagatgtt gcgctccgag
      241 atcatgatgc cggccttgtg gtacttgtac aggtccatgg cgatctcgag gtctccgcgc
      301 cgcacccttc ggccgagcct tgctaccgac gcccagtttt cccagaactg cttctgaaat
      361 atattggcca tattcctatt cagaggggtg gcggccacgg tcgactcgac gccggcggtc
      421 gcgaagaaca ggctccacgc aacggccatg tgcttcatga acatgttgac cttcgacagc
      481 gtcttcaaca cgtcgtgctc aaactcctcc ccgatctcca cgttccattg cgccggcctc
      541 ccaacgacgt gctggaacca ataccgcgcc cggctgtcgt accccgcctc tttcttcaac
      601 acgtagaaag cctggataga ttcgtaatcg ctcttgacca gctcgtagtt gtcgaggttt
      661 acggcgcgct cgttcccgct gaccaggctg cggagcttct cgtgcagcgt gtccccgcgc
      721 ccggcacgcc ccgccttgcg ctccagcatc cgaagctgtt ttcgaagcgt gtcctcccgc
      781 agcatgctgc ggatgtctct tccgcgattc gccgggtcgg tatctttgac cccctctctc
      841 gggatcgcga tgacgaacgg attctgcgcg gcgtcctcgg ccagcatgaa ttgattcacg
      901 aggacttccc tcgtgagcgt gtccatcatg tctttgatgg acgtctctgc gagacgcagc
      961 gggtccgata tcgcgggcat cagcgcggac actttttcct tgtcgtttgt ggactgtatg
     1021 aaagcgtcgt agtatgacgg gtactcccgc tttcctcgac gagccgtcca ctcccgttcg
     1081 gagtagtctc gtatggactg ctgcagcccc cggataagtt ctgcagttcc ttcggtgccg
     1141 ccctgcgtcg ggtccccgat tttgtagtgc tccgccccga ccagctccgc ccgctccgtt
     1201 tctacgcggg tagccaagcg gaggagcgcc cggttgtaca tgatcttgct ccacgggctg
     1261 tccttgtctt tgcgctcctt ctccgtggcc agcgccattc tcggggcctt ctttttcttc
     1321 tttcggtaca gatggtccaa ttcgtcggag agctgaacat ataaagcttc ctgctcgggg
     1381 acggcttctt tgtcggttcc aacgtctttg aagcttgcct tgagcgctgc cggcggaagc
     1441 cttcgctgcc caccagctcg agggcctgcg ctctctcgca cttgcctgtg caccgcctcg
     1501 cgcgccttgc gccgcccggc ggcttcgaat ccatgcccgc cgaagacatg cggcaggtag
     1561 cgttggattt ctccgacgac gccttcgctg gcggcgcgac cggtccacgc ttctatatcc
     1621 cggtagtccg ccatggcgtc gcgaagttct ttctgtatgg tctttgcgta ctcgacgagc
     1681 ccgccctcga ggctgccgtt ctcgccgcgc cggatatcta tcatggacag cggacgcccg
     1741 tattcttgga gggtcttaaa gtaatccgac tccatcagtt tctttatggc caagtcgacg
     1801 ctcatgaacg cagaggtcgg cctgtgctct ttcgcgtccc atccgtcgac ttgccgtatg
     1861 cgctttgcac ccgccctgtc ttctgccacc acctcgccgt tgaacaccca gacgtactct
     1921 gacggccgct cttcgccatc ttcccccagt tcggtcttgg ctccgcggta gctgtccgtc
     1981 ccaaaggtga ggtattcttt gccctcgatg atagccatta ccaggtagcc cagcagctct
     2041 tgttcgttca gctcagcgtc agtcagctcc cgcaggttgt cttttgcccg gttgtatatc
     2101 ttatcctgat agtcatagac gtccctcccc agcttcttgg agtccttctc gttcttcacc
     2161 attcgaacga tagtccggcg ctcccgcccc tccccgaact tccggccctc cgcacgaatg
     2221 accatgttga ggcggttctc ccaagcggac acgagcccga gcagccgcgg aatttcttct
     2281 ttcaagtaat ccaccccgcc gatggcagct cgctgagcgg agtacattga aatgagctcc
     2341 caatgcccaa ccaaagaccc cgtcgtcatg tcgttgtcgg tacgtgaatt gtcgcctatt
     2401 ctccgtacgt cgtaatccag cgaagcacga acgatcgccc ggtccagccc gaaccgatct
     2461 tttcgcgata gatttcgttc gagttctact tctctgtgcg cggcagcgaa gtcaccaacc
     2521 gcagccgctg cagccacatc agctaccagc gcgcgtatct tctgatcttt tgccgaccac
     2581 gagtccccag ttcgctccgc agctttcgca agaagccacc cgccggcggc gaacaccggg
     2641 cccgcggctt cccagttcgt gatgtctgga cgaagcgact tggccaggtt ctcctggagg
     2701 cctacttcgt cgaacacgcg gcttgaaaac aggtcttctt cccctgcgcg cacgcggcgc
     2761 attcgattgc gccactccgg gctcgcctta acttccaaaa tcttctgcag cccactagcg
     2821 accaagtgcc tttgttctga cagactcgcc ttatctatcc tctctccgag cccaagccca
     2881 agcacgggct ccgctttcgc ttcgtccaag cgtcgcttgg cttctttata cattcgttcc
     2941 atggtgacaa gggactgcgg gtgcatagat tcgaaacgct gtcgctctgt ctctttgagc
     3001 atcggcgatc gaacgatccg ggccgccttg cgcattctgt cgaattcttt cccgacgact
     3061 ttcgcatacg acggcttgcc tacgctcttg agctcctctt tgatggagct taccagcggg
     3121 tcgtggaaca ctccgagctt ggccaggata tccgcct
//
LOCUS       DAOSWW010000216         3152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22488_length_3152_cov_3.309655, whole genome shotgun sequence.
ACCESSION   DAOSWW010000216 DAOSWW010000000
VERSION     DAOSWW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22488_length_3152_cov_3.309655"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PK442_05475"
     CDS             complement(<1..519)
                     /locus_tag="PK442_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO87133.1"
                     /translation="MKERTSKIVAELFAPGDALEGIRESALQAGELLRDSFAGGGKLL
                     VCGNGGSWADGEHIVGELMKGFLLKRPLPDDAKRMLTLLWGDEGRTIGEALQGALPAI
                     SLGSHGALMTAFANDADGRFAFAQQAMGYACPGDVALGISTSGNAENVRFALMAAKSR
                     GATTVALTGADGG"
     gene            complement(516..1508)
                     /locus_tag="PK442_05480"
     CDS             complement(516..1508)
                     /locus_tag="PK442_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOO87134.1"
                     /translation="MYAIGVDIGGTKCAVCLGKARGDTLRVVHKCELRKTAEYSPAEM
                     LDALLADVRECRSRIPARETVIGTGISCGNPLDSRAGVILSPPNLPGWDRIEVVDWFR
                     SGTGLPAWLCNDANAGALAEWRYGAGRGCEHLIFLTFGTGFGAGLILNGRLYAGACDA
                     AGETGHVRAAAHGPVGYGKIGSYEAFCSGGGIAQIARTYALRDIQNGKPSALCPDVAG
                     LESLTAESTARVAREGNPTAKEVFELSGAEFGRALSMLIDLFNPQRIVVGGIFSRCRD
                     LIWPAAERVIAEEALPDSRKACTVLPCELSESIGDMAALSVAVYYWEHGGEGKR"
     gene            complement(1613..2521)
                     /locus_tag="PK442_05485"
     CDS             complement(1613..2521)
                     /locus_tag="PK442_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO87135.1"
                     /translation="MKKAVAVFLGCVLLAGLFAGGAVAAEKHKVYLITMDQMDQHWVT
                     VNAGAEKAVAELGTIDYKWLAPDVKDDAKQIEAVNNAVAGGAEVLLVAANGPTAISAA
                     LKEASEAGVKIVYVDSPADFPAIQTLATDNEAAGVTAGKEMLKALEAAGVAEGKIGIV
                     NVNAATASTVAREKGFRAAFEGTKFELLETQYCDGDAARAKDIASNYITQGCVGIFGA
                     NEGSTVGTGNAIQEAGGKVVGVGFDKSNMILSLIKNGFLVATMAQNPDVMGYEGVKTA
                     AKALAGEPTGAEYVDTGVSVLTKNDL"
     gene            complement(2635..>3152)
                     /locus_tag="PK442_05490"
     CDS             complement(2635..>3152)
                     /locus_tag="PK442_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87136.1"
                     /translation="GRLGGFFNTIWIALALWLVFNFLLSRTRLGRHIYATGSNKEAAR
                     LSGVDVVASTTAAYMVSAVCSCVVGLIVCATSGMGTMDAGNMYELYAVAASVIGGVST
                     LGGQGLLLGTVMGASIWGVLQNGLQFAGAPVAIRNIVIGVIVVVSVLLDVVVRSEKRR
                     RKKERKEEAKG"
BASE COUNT          526 a         1125 c          909 g          592 t
ORIGIN      
        1 ccccccgtcc gcaccggtca gcgcgacggt cgtcgcgccg cggctcttcg ccgccatcag
       61 ggcgaaacga acgttctccg cattccccga cgtgctgata cccagcgcga catctccggg
      121 gcacgcgtac cccatcgcct gctgcgcgaa ggcgaaccgt ccgtcggcgt cgttggcgaa
      181 ggcggtcatc agcgctccgt ggctcccgag ggagatcgcc ggaagcgctc cctgcagcgc
      241 ctcaccgatc gtgcgccctt cgtcccccca gaggagagtt aacatgcgct tcgcatcgtc
      301 cgggaggggg cgtttcagaa ggaatccttt catcagctcg ccgacgatgt gctccccgtc
      361 cgcccagctc ccgccgttgc cgcagaccag cagcttcccc ccgccggcaa aagagtccct
      421 cagcagctct cccgcctgca gcgcgctctc gcgaatgcct tccagcgcgt cgcccggcgc
      481 gaacagctcc gcgacgattt tcgatgtacg ctctttcatc gttttccctc gcctccgtgt
      541 tcccaataat acaccgcgac ggacagggcc gccatatccc cgatggattc cgacagctcg
      601 cagggaagca ccgtgcaggc tttccgggaa tcgggaagcg cctcctccgc gatcacgcgc
      661 tcggcggcgg gccagatgag atcgcggcag cgtgaaaaga tgcctccgac gacgatacgc
      721 tgaggattga agaggtcgat gagcatcgag agcgcgcgtc cgaactccgc cccggagagc
      781 tcgaaaacct ctttcgccgt tggatttcct tcgcgcgcga cgcgggctgt cgactccgcg
      841 gtcagcgatt ccaggccggc gacatccggg cacaacgcgg acggcttccc gttttggata
      901 tcgcgcaggg cataggtccg ggcgatctgg gcaataccgc ctccgctgca gaacgcctcg
      961 tacgacccga tttttccgta cccgacggga ccgtgcgccg cagcgcggac gtgccccgtc
     1021 tcccctgcgg cgtcgcacgc tccggcgtag agacggccgt tgaggatcag ccccgcgccg
     1081 aacccggtgc cgaaggtcag gaagatcagg tgttcgcacc ccctcccggc gccgtaccgc
     1141 cactccgcca gcgcacccgc gttggcgtcg ttgcagagcc acgccggaag cccggtcccg
     1201 ctccggaacc agtcgacaac ttctatgcgg tcccaaccgg gcaggttcgg gggggacaat
     1261 atcacccccg cccggctgtc gaggggattg ccgcagctga ttcctgtgcc gatgaccgtt
     1321 tccctcgccg gaatcctcga tctgcactcc cgaacgtcag cgagcagtgc gtcgagcatc
     1381 tccgcaggcg agtactccgc ggtcttcctg agctcgcact tgtgcacgac gcgcaaagta
     1441 tctcctctgg ccttcccgag gcatacggcg cacttcgttc ctccaatatc gacgcctatc
     1501 gcgtacattc ccatccctcc gagctcacgg gagctcccgc aagggcgctc ctactacaga
     1561 aggcccccgc cctgagggac gggggccttc ttcatactcc ggtacgctcc ttctacaggt
     1621 cgttcttcgt cagaacggag acgccggtgt ccacatactc ggctcccgtg ggctctcctg
     1681 caagagcttt ggccgcggtc ttcacacctt cgtaccccat gacgtcgggg ttctgcgcca
     1741 tggtggcgac gaggaagccg ttcttgatca ggctgaggat catgttcgac ttgtcgaagc
     1801 ccacaccgac cactttgccg ccggcctcct gaatggcatt gccggttccg acggtcgagc
     1861 cctcgttggc tccgaagatg ccgacgcacc cctgggtgat gtagttggag gcgatgtcct
     1921 tcgcccgtgc ggcgtcgccg tcgcagtact gcgtctcgag cagctcgaac ttcgtccctt
     1981 cgaacgccgc gcggaagccc ttctcgcggg cgaccgtcga cgcggtggcc gcgttgacgt
     2041 tgacgatgcc gatcttgccc tcggcaactc cggccgcttc aagcgccttc agcatctcct
     2101 tgcccgcggt gacaccggcg gcttcgttgt ccgtcgccag cgtctggatc gcggggaagt
     2161 ccgcgggaga gtcgacatag acgatcttga cgcccgcctc ggaagcttct ttcagggccg
     2221 cgctgatcgc cgtcggtcca ttggccgcca cgaggagcac ttcggctccg ccggcaaccg
     2281 cgttgttgac cgcttcgatc tgcttggcgt cgtccttcac atcgggagcg agccatttgt
     2341 agtcgatcgt gccgagttcc gccaccgctt tttcagctcc ggcgttcacc gtcacccagt
     2401 gctggtccat ctgatccatg gtgatcagat acaccttgtg cttctccgcc gccaccgcgc
     2461 cgccggcaaa aagcccggcc agcaaaacgc atcccagaaa caccgctact gcttttttca
     2521 ttcgtccgtt cccccttgaa gatatgatgt gaaaccgttg tatcaaaaga agcggagagt
     2581 tccgtctctc cccggaagcg ctgcgtcgaa caggcctcgc tccggggaag agcatcagcc
     2641 cttcgcctct tctttccgtt ccttttttct gcgccgcttc tcgcttctga cgacgacgtc
     2701 gagaagaacc gagacgacca cgatcacgcc gatgacgatg ttgcgtatcg ccacgggggc
     2761 ccctgcgaac tggagtccgt tctgcagcac gccccagatc gaggcgccca tcaccgttcc
     2821 cagcagcagc ccctgtcctc cgagcgtgct cacgcccccg atgaccgagg ccgccacagc
     2881 gtacagctcg tacatattgc ccgcgtccat cgtccccatg ccgctggtgg cgcagacgat
     2941 cagcccgacg acgcaggaac agacggcgct gaccatgtac gcggccgtcg tcgacgcaac
     3001 gacgtcgacg cccgagagac gcgccgcctc cttgttgctc ccggtggcgt agatgtgccg
     3061 ccccaggcgt gttcttgaaa gaagaaagtt gaagaccagc cagagagcca gggcgatcca
     3121 gattgtgttg aaaaagccac ccagcctgcc gt
//
LOCUS       DAOSWW010000217         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22536_length_3148_cov_3.612997, whole genome shotgun sequence.
ACCESSION   DAOSWW010000217 DAOSWW010000000
VERSION     DAOSWW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22536_length_3148_cov_3.612997"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="PK442_05495"
     CDS             <1..2134
                     /locus_tag="PK442_05495"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO87137.1"
                     /translation="LCERMQLGAPDASGRRSPVPTGETFVIEVDTVIAAVGQGIDFST
                     VPAELHDGRRMKVGKDYDTPLPGVFVCGDQQTGPKIAIEAIGNGHWAADSLDHYLMHG
                     TPKKPFFYDIVQTDLGPEDFADVERTTQEHAAHVSGETRLAAPFKEYSKGLTEEQTLR
                     DAKRCMECGCPDVFECKLRKFATTHEVQPEKLAGAHIEKFEDANQYYVRNMDKCVLCA
                     KCVRACDEISGFHAIDFAKRGFESVLTPHFFNDMEHSDCTFCGLCTQVCPVGALMEKR
                     VERWPHLEEPTIVKTTCMLCPVGCELDLNLDRKRSRIVRVTTDLDNPEAPTGGSCCVK
                     GRYAFKDVADDGAFYPALKGACSSWSDAVSAFEELASGNSPAFVLGPSLTLEEVRALK
                     EYTALRAPNAEMYAIWDAGFISLLNEAAKRKDVVRATYASMTGPSPSSDIRCGEADAF
                     LLVGTNTDEDQPVLTSWLRRSARHRKSAVVYIGETPGLLDKGEAVVLHPEAGKEAQVL
                     TALTSAVKAITAKESVASTNLLSGTGVLPEDLEKAAGLLAGAKHPVTLIGAEASAKEV
                     FDAAAALAGGRYMLLFKGSGSVALISAIPGILPAATLRTVQKNMGALLFVGTTPEDAG
                     FTADSLTERKYAVLSSVSSPLAEKADVLLPLLPWIEKDGSITNLEGRTLSVRRGPLTK
                     KTGKSLSALLAEAALPYEGRIHANPVAR"
     gene            2305..>3148
                     /locus_tag="PK442_05500"
     CDS             2305..>3148
                     /locus_tag="PK442_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO87138.1"
                     /translation="MHSGIFCVIFALVLLFAAASSAATPSGDPIKIGYLATLTGDGAT
                     WGQHERDGALLAVKELNEAGGVLGRPLELVYYDVRGRQEDAIQAARRLIHEDKVVAIG
                     GTNYSGLNIAIASVVNSGEVPQIGTASTNPAVTVDPKTNTVRPYTFRLCYTDPYQGKV
                     MADYLVNKLGVKSTAVITDIGSDYSEGLKEFFLARIEELGCEMKGVWGFRGGDVDFRA
                     QLTEMKATGAEAVALPILYKEMGLIMKQAAELDWKPIFIGGDGFSPSMHEIAGDAMEG
                     SYWVY"
BASE COUNT          707 a          951 c          899 g          591 t
ORIGIN      
        1 cctctgcgaa cgcatgcaac tcggcgcccc cgacgcttcc ggcagacgga gccccgtccc
       61 cacgggagag acgtttgtca ttgaagtaga caccgtcatc gctgcagtcg gtcagggaat
      121 cgacttttcc accgtgcccg cagagttgca cgacggcagg aggatgaaag taggcaagga
      181 ttacgatacg ccgcttcccg gagtttttgt ctgcggcgac cagcagactg gccccaaaat
      241 cgccatcgag gcgatcggca atgggcattg ggcggcggat tcgctggacc actacctgat
      301 gcacggaacg ccaaaaaaac cgtttttcta cgatatcgtc cagacggacc tcggccccga
      361 ggacttcgcc gatgtcgaac gtacaaccca ggagcacgcg gcgcacgtat caggagaaac
      421 gcggctggcc gctcccttca aggagtacag caaagggctg accgaggagc aaaccctccg
      481 cgacgcaaaa cgatgcatgg agtgcggttg tcccgacgtc ttcgaatgca agctccgcaa
      541 attcgcgacc acgcacgaag tgcagccgga gaagctggcg ggcgcgcaca tcgagaagtt
      601 cgaggacgcc aaccagtact atgtgcggaa tatggacaag tgcgtcctct gtgcgaagtg
      661 cgtccgcgca tgcgacgaaa tctcaggttt ccacgccatc gactttgcga agcgcggatt
      721 cgagtccgta ctcacaccgc acttcttcaa cgacatggaa cactccgact gcactttctg
      781 cggcctctgc acccaggtct gcccggtcgg ggcgcttatg gaaaaacgcg tcgaacgctg
      841 gccgcacttg gaagagccta caatcgtcaa gaccacatgt atgctctgcc cggtcggctg
      901 cgagcttgac ttgaaccttg acaggaagcg ttcccgtatt gtccgcgtta ccacagatct
      961 cgacaatccg gaggcgccga caggcggttc ctgctgcgta aaggggcgct acgccttcaa
     1021 agacgtcgcc gacgacggcg ccttctaccc tgcgctcaag ggagcttgct cctcctggag
     1081 cgacgccgtc tcggctttcg aggaactcgc atccgggaac agccctgcgt tcgtactcgg
     1141 tccatcgctc acactcgaag aagttcgcgc tcttaaggag tacacggccc ttcgcgctcc
     1201 gaacgcggaa atgtacgcga tttgggatgc cgggttcatt tcccttctca atgaagcggc
     1261 gaagcgtaag gatgtggtca gggcgaccta cgcctccatg accggcccct ccccgtcaag
     1321 cgacatacgc tgcggagagg ccgacgcctt cttgctcgta ggaacgaaca ccgacgaaga
     1381 ccagccggtg ctcacatcct ggctccgcag gtctgcccgt caccgcaaat cagcggtggt
     1441 ctacatcggc gagactccag gccttctgga caaaggcgaa gccgtagttc tgcacccgga
     1501 agccggaaaa gaagcgcagg tcctcacagc gctcacatcc gcagtcaagg cgataacggc
     1561 caaggaaagc gtcgcgtcga ccaatctcct ctcaggaacc ggcgttcttc ccgaagacct
     1621 tgaaaaagcg gccggactcc ttgccggtgc gaaacatccc gtgacgctca tcggcgcgga
     1681 ggcgtctgca aaagaagttt tcgacgcggc tgcggcgctc gcaggaggac gatatatgct
     1741 tctcttcaag ggatcgggaa gcgttgcgct catctcggcc atacccggga tacttcctgc
     1801 ggcaactctt cgcacagttc agaagaacat gggcgccctg ctcttcgtag ggacgactcc
     1861 cgaggatgcc ggcttcaccg cggacagcct gacagagaga aagtatgcgg tcctttcgtc
     1921 cgtatcctct ccgctcgccg agaaggccga cgttctgcta ccgcttctcc catggattga
     1981 aaaggatgga agcatcacga acctcgaagg acgaacgctc tcggtccgac gggggcctct
     2041 gactaaaaaa acaggaaaga gcctctccgc ccttcttgcg gaagccgctc tgccttacga
     2101 gggaagaatt cacgccaatc ctgttgctcg ctgatacaaa aacacaaaaa aagaacgagc
     2161 gtcatcaaaa ttcacagggc cttccggccc tgtgaatttt tgtttctttt tattagccgt
     2221 attgacacaa agggcaacgg cctttagaat taaccaaata tttttttaac acatatcagg
     2281 gaggttgaga aattcttgag acgaatgcac agtggcattt tttgcgtcat cttcgcgctg
     2341 gtactgcttt tcgctgccgc ttcttctgcg gcaacaccgt cgggagatcc gatcaagatc
     2401 gggtatctgg cgacgctcac cggagacggc gccacttggg gccagcatga acgcgacggc
     2461 gcgctgctcg cggtcaaaga gctgaacgag gcgggcggcg ttcttggacg gccgctcgaa
     2521 ctggtctatt acgacgtccg cggacgacag gaggacgcta tccaggcggc ccgccgtttg
     2581 attcacgaag acaaagtcgt tgcgatcggg gggacgaact acagcggcct caatatcgcc
     2641 atagcctccg ttgtgaacag cggcgaagtc ccgcagatag gtaccgcgag caccaacccc
     2701 gcagtcaccg tcgatccgaa gacaaacaca gtgcgcccct acacgttccg tctctgctac
     2761 accgacccct accagggaaa ggtcatggcc gactacctcg tcaacaagct cggcgtcaaa
     2821 agcacggccg ttatcaccga cattggaagc gactactccg aagggcttaa ggagtttttc
     2881 ctcgcacgca ttgaagaact cggctgcgaa atgaagggtg tttggggttt ccgcggcggc
     2941 gacgtcgact tccgggccca gctgacggag atgaaagcga ccggcgccga ggcggtcgcg
     3001 ctccccattc tctacaaaga gatgggcctg attatgaagc aggcggcgga actcgactgg
     3061 aagccgattt ttatcggagg cgacggcttc agcccgagta tgcacgagat cgccggcgac
     3121 gcgatggagg ggagctactg ggtctaca
//
LOCUS       DAOSWW010000218         3140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22639_length_3140_cov_2.906969, whole genome shotgun sequence.
ACCESSION   DAOSWW010000218 DAOSWW010000000
VERSION     DAOSWW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22639_length_3140_cov_2.906969"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..532)
                     /gene="ilvN"
                     /locus_tag="PK442_05505"
     CDS             complement(41..532)
                     /gene="ilvN"
                     /locus_tag="PK442_05505"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOO87139.1"
                     /translation="MERTIGVVAENRPGVFSRISGLMERRGFQVKGVTAGVTQQPGRS
                     RFTLVIAGDDRKASQAVRQIRKMVETVEVSDLSSGFCVERCMMLMKFSLPAERRAELF
                     DAMNAFPHTVASEHGDTVILETTGTGENLDACLETVRHLGLVEAVKSGPLALDARTTT
                     TTF"
     gene            complement(669..878)
                     /locus_tag="PK442_05510"
     CDS             complement(669..878)
                     /locus_tag="PK442_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87140.1"
                     /translation="MPAIRTARNERPSRRTPWYHTVGKDDIEAKEETPVKNMSTTTHS
                     ALTFVVAGAIIIIRCGDSPGALLVV"
     gene            957..2048
                     /gene="pheA"
                     /locus_tag="PK442_05515"
     CDS             957..2048
                     /gene="pheA"
                     /locus_tag="PK442_05515"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOO87141.1"
                     /translation="MDVEKLRECREKINEIDGSIAALLSQRADVAREVGRAKGDAPVY
                     DPAREGEVIARAAEACPGMDRSAVERIYREIISVCRGVQHRPKVAFLGPEGSFSHEGA
                     LAAFGGAIDLLPCLSFVDVFTAVEKRSADWGVVPAENSLEGTVVPTMDAFASQAGLLD
                     SSSHNNICIHSEVSIAIDHHLASPEKNLDAIREVFSHPQALGQCREWLRTNLPSASQI
                     PAGSTSAAAASVAGAPGRAAVCSALAARNNGLNILAEKIQDRGRNTTRFWTVGFGTPR
                     KGERNKTSFLFSVEHRPGSLFRAMEPLYLNGLNLTHIQSRPLSGSVFEYYFFIDVEGH
                     IDEPHVRKALDVMRERTAFLRLLGSYPCL"
     gene            complement(1993..2556)
                     /locus_tag="PK442_05520"
     CDS             complement(1993..2556)
                     /locus_tag="PK442_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOO87142.1"
                     /translation="MHSGNICIGGFMCSGKTSVGRELSRRLGWEFLDTDVVIEQRAGM
                     PVPRIFAELGEPVFRRFEFEVVSEAVGGSNRIVSLGGGALVNTELRNRVLSNACLVIL
                     DVRAETVLCRAERQKGARPLLDSGTVHSLMAKRTSAYASCHFRVATDEFPVNAVADRI
                     LANIPLTGTLTGTDTIRAAAETLCVPA"
     gene            complement(2549..>3140)
                     /locus_tag="PK442_05525"
     CDS             complement(2549..>3140)
                     /locus_tag="PK442_05525"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOO87143.1"
                     /translation="DEETLKKRILQAAEEGDSLGGTFVLSITGLPPGVGSFAEWDERL
                     DGLLGQAILSIPGVKGVEFGEGFHSADLPGSRLHDPIVVDGGRWTRPSNNAGGLEGGM
                     SNGGEIRLRAVMKPIPTMKKGLPSFDTVSDRPCAGHSERSDVCAVPSACVVAEAMAAW
                     TVACAVTAQFGNDRMDDLAQRFASYKQRAERWNLHA"
BASE COUNT          541 a          985 c         1009 g          605 t
ORIGIN      
        1 ctttgccata atcagtccaa ctccttttcg aatgtgtgct ttagaatgtg gtggtcgtcg
       61 tccgcgcgtc gagggccagg gggccgctct tcaccgcttc gacgagcccg agatgccgaa
      121 ccgtttcgag gcacgcgtca agattctcgc ccgtgccggt ggtttcgagg atcaccgtgt
      181 cgccgtgctc gcttgccacg gtgtggggga aggcgttcat cgcgtcgaag agctccgcgc
      241 gccgctccgc cggaagggag aacttcatca gcatcatgca gcgctccacg cagaagcccg
      301 agctgagatc ggagacttcg acggtctcca ccatcttgcg tatctgccgc accgcctggg
      361 acgccttgcg gtcgtcgccc gcgatgacga gggtgaagcg ggaacgcccc ggctgctgcg
      421 tgactccggc ggtaacgccc ttcacctgaa atcccctgcg ctccatgagc cccgatatgc
      481 gtgaaaaaac tcccggtctg ttttccgcga cgacgccgat ggtccgttcc atgccgctct
      541 cctcctctta tcttgtgtat gcccctctcg ggagctctct cttcgcctgc gaaggcggta
      601 tctgcgcata aaaaaagccg gggttcccgt agagtgcggg aaccccggcc ggaaggctct
      661 tcgtcggtct acacgacgag aagcgctccg ggggaatccc cgcagcgaat aataataatg
      721 gcgccggcta cgacaaatgt aagggcggag tgcgtcgttg tggacatatt cttcacaggg
      781 gtttcctcct tcgcttcgat atcgtctttc ccgaccgtgt ggtaccatgg agttctccga
      841 gaggggcgct cgttccgcgc cgttcggatt gcgggcaatc ttataggcct ccttcgaaaa
      901 tgtcaagggg ggttgaaata aaagataatt ctaaaatttt cagagggggt tgttctatgg
      961 acgttgaaaa gcttcgtgaa tgccgggaaa agatcaacga gatcgacggg agcatcgccg
     1021 cgcttctttc gcagcgggcg gacgtcgccc gcgaggtggg gagggcgaag ggggacgctc
     1081 cggtgtacga ccccgcgagg gagggcgagg tgatcgcgcg cgcggccgaa gcctgccccg
     1141 ggatggaccg gagcgcggtc gaacggatct accgcgagat catctccgtc tgcagaggcg
     1201 tgcagcaccg cccgaaggtc gccttcctcg gccccgaggg ctccttctct cacgagggag
     1261 cgctcgcggc cttcggcgga gcgatcgatc tgctcccctg cctctccttc gtcgacgtct
     1321 tcaccgccgt ggagaagcgc tcggcggact ggggggtggt tccggcggag aactcgctgg
     1381 aggggacggt ggttccgacg atggatgcgt tcgcgtctca ggcggggctg ctggattcat
     1441 cttctcataa taatatttgc atacacagtg aagtttcgat tgcaatagac catcatctcg
     1501 cctctccgga gaaaaatctt gacgcaattc gggaagtttt ttcgcatcct caggccctcg
     1561 ggcagtgcag agagtggctg cggacgaacc tgccgtctgc gtcgcagatc cccgcgggaa
     1621 gcacgagcgc ggctgcggcg tcggtcgccg gggcgccggg gcgcgccgcg gtgtgcagcg
     1681 cgctcgcggc acggaacaac ggcctgaata tcctcgcgga gaagattcag gacagggggc
     1741 ggaacacgac gcgtttctgg actgtgggat tcggaacccc ccggaagggg gagaggaaca
     1801 agacgtcgtt tctgttcagc gtggagcacc gcccgggaag tcttttccgg gccatggagc
     1861 cgctctatct gaacgggctg aaccttaccc acattcagtc gcggcccctt tcgggaagcg
     1921 tcttcgagta ctacttcttc atcgacgtcg aggggcacat cgacgagccc cacgtgcgga
     1981 aggcgctcga cgtcatgcgg gaacgcacag cgtttctgcg gctgctcgga tcgtatccgt
     2041 gcctgtaagc gtccccgtca gggggatgtt cgccagaatc cggtcggcca cggcgttcac
     2101 gggaaactcg tccgtggcca cccggaagtg gcacgacgcg taggcggacg tccgtttcgc
     2161 catcagggaa tggacggtgc cgctgtcgag cagggggcgc gcgcccttct gccgttccgc
     2221 ccggcagagc accgtctcgg cccgcacgtc gaggattacg agacaggcgt tcgagagcac
     2281 tctgttccgt agctccgtat tcaccagcgc gccgccgccg agcgaaacga tgcgattgct
     2341 tccgccgacc gcttcggaga ccacctcgaa ctcgaaccgc cggaagaccg gctcgccaag
     2401 ctcggcgaag attctgggga cgggcatccc cgcccgctgc tcgatgacga cgtccgtgtc
     2461 caggaactcc catccgagtc tgcgggaaag ctcccgcccg acgctcgtct tgcccgagca
     2521 catgaagccg ccgatgcaga tattcccgct atgcatgaag attccacctt tccgctcgct
     2581 gtttgtagga tgcgaaacgc tgcgcgaggt cgtccatcct gtcgttgccg aactgtgcag
     2641 tcaccgcgca cgccaccgtc cacgccgcca tcgcctccgc gacgacgcat gcagacggaa
     2701 cggcgcagac gtcgcttcgc tccgagtgtc cggcgcaggg ccgatccgat acagtgtcga
     2761 acgagggaag cccctttttc atcgtcggga tcggcttcat cacggcgcgg aggcggattt
     2821 cgccgccgtt gctcatgccg ccttcgagtc ctcccgcgtt gttgctcggg cgggtccagc
     2881 gtccgccgtc gacgacgatg gggtcgtgga ggcggctgcc gggaaggtcg gcgctatgga
     2941 agccctcgcc gaactcgacg cccttgacgc cggggatgga gaggatcgcc tgtccgagca
     3001 gcccgtcgag ccgctcgtcc cactccgcga agctcccgac gccggggggg agtccggtga
     3061 tcgaaaggac gaaggtcccg ccgaggctgt cgccctcttc ggcggcttga aggatgcgct
     3121 ttttcagcgt ctcttcatcc
//
LOCUS       DAOSWW010000219         3138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22657_length_3138_cov_3.759325, whole genome shotgun sequence.
ACCESSION   DAOSWW010000219 DAOSWW010000000
VERSION     DAOSWW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22657_length_3138_cov_3.759325"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PK442_05530"
     CDS             complement(<1..1248)
                     /locus_tag="PK442_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HOO87144.1"
                     /translation="MARDIGVTECDVPMEGEAVLRKATKECAYRTEMDPMWTKAIVVL
                     SCVFILYHLVTSRFGMPPMFKHRAIHLGFILCLVWMYYPATSRSIQKRPSFVDMGLMV
                     ASIAVCGYTVLNVDAFALRGGVALPRDYYFGAVAILLVLEACRRVAGKGLLILAVVFL
                     LYAWLGRYIPGVLSHRGYSIQRIIYQMYLTTEGIYGLPIGIAATYLVLFIVFSSFLEK
                     SGLGTLFNDVSLALGGRLVGGPAKVAVIGSALVGMISGSAATNVATSGAFTIPLMKKI
                     GYKPYFAGAIEAAASTGGQIMPPIMGTGAFIMAEFLGVPYISIAAAAVIPAVLYFAAV
                     FFQVDLRARKIGLTGLSKEELPDVKKTLLRYGHMFLPIIILVVLLMQHFTPLYAAFYS
                     ILVTWLLSFLRKETRMGVGKLVPM"
     gene            complement(1460..2434)
                     /locus_tag="PK442_05535"
     CDS             complement(1460..2434)
                     /locus_tag="PK442_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOO87145.1"
                     /translation="MVRIIGRDDIKTAAGRISGRVRRTPVLRGDKLDALFGAEVYFKP
                     ENLQLTGSFKIRGAMNKILSLSDEERAKGIIASSSGNHAQGVAYAAKMLGIKATLVLP
                     ENAPKSKIEGTRALGADVVLHGFDSIQRYKKLYEIKAEKGYTLVHSFNDPELIAGQGT
                     SGLEIIEDLPDVDTVVVPLGGGGLLAGVATAIKETRSSVRIVAVEPESIQRYAESRKA
                     GKPVEVPMGATLADGLMITMTGEHTYPLIDKYVDEIVPASDEFIRKALMEIVFKSKLL
                     VEPSASVGVAAALQGSFKVKPGEKICFFLSGGNIDPEKLASFLPRETA"
     gene            complement(2543..2743)
                     /locus_tag="PK442_05540"
     CDS             complement(2543..2743)
                     /locus_tag="PK442_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO87146.1"
                     /translation="MAVHVNKRLCKGCGLCISVCPKNVYEISNETNQKGFTVVAAVRE
                     GDCIKCKRCEITCPDMALWIEE"
     gene            complement(2750..>3138)
                     /locus_tag="PK442_05545"
     CDS             complement(2750..>3138)
                     /locus_tag="PK442_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOO87147.1"
                     /translation="VGKTLTDAGIGQSTVVALGGGPIWGTNQREVVELFNADPETEII
                     VLLGEIGGGTEIAAAEYIAEYVRKPVVSLIVGRAAPEGKSLGHAGAIIRGNKGTAASK
                     MEALEKAGAHLATSPAQVVEIIRKLG"
BASE COUNT          627 a          963 c          885 g          663 t
ORIGIN      
        1 catggggacg agtttgccga ctcccatgcg cgtttccttt cgcaggaacg agagcagcca
       61 ggtgaccagg atcgagtaga atgccgcata gagcggtgtg aagtgctgca tgagcagaac
      121 gacgaggatg atgatcggca ggaacatgtg cccgtaccgg aggagcgtct ttttcacgtc
      181 gggcagttcc tccttggaaa gccccgtcaa accgatcttg cgcgcccgga ggtctacctg
      241 gaagaagact gctgcaaaat agaggaccgc cggaataacc gccgccgccg ctatcgaaat
      301 gtaaggaact ccgagaaact ccgccatgat gaacgctccg gtccccatga tcggcggcat
      361 gatctgccct cctgttgagg ctgccgcttc aatagctccg gcaaaataag gcttataccc
      421 gatttttttc atcagcggga tggtgaaggc gcccgacgtt gccacgttgg tggccgcgct
      481 gccgctgatc ataccgacaa gagcgcttcc gataacggca acctttgcgg gcccgccgac
      541 cagacggcct ccgagcgcga gggagacgtc gttgaagagc gttccgagtc cgcttttctc
      601 aaggaaagag gagaagacga tgaagaggac aaggtatgtc gcggcaatgc cgatggggag
      661 tccatagata ccttccgtgg tgaggtacat ctggtagatg atgcgttgga tcgagtagcc
      721 ccggtgggag agaacgccgg gaatgtaccg gccgagccag gcgtacagca ggaagacaac
      781 ggccagaatg aggagtcctt tccctgcgac tctgcggcag gcctcaagga cgagcagtat
      841 cgccaccgcg ccgaagtagt agtccctggg aagcgcaacg ccgcctctga gcgcgaaggc
      901 gtcaacgttg agcaccgtgt agccgcagac ggcgatggag gcgaccatca gccccatgtc
      961 gacgaaactc ggtctcttct ggatggagcg gctcgtcgcg gggtagtaca tccacaccag
     1021 acagagaatg aatccgagat gaatcgccct gtgcttgaac atgggaggca tcccgaacct
     1081 ggaggtgaca agatgataga gtataaacac gcatgacagg acaacgatcg ccttcgtcca
     1141 catcgggtcc atctccgtcc tgtacgcgca ttccttcgtg gcctttctca gaacggcttc
     1201 cccttccatg gggacatcac actcggtgac accgatatct cgcgccattc atttccctcc
     1261 actcctgaaa tatttactcg ttcatgcttt atgcattata tattgtatat gaagctcaaa
     1321 ggcattgcaa agtgcatact gtattcttga gtggtacagt gaactctctt tacgatacgt
     1381 ctggcaaaca ctggcggaaa agcggcggga aggggtgcgt tttcatggcc ggctcgttcc
     1441 gcttcgccgc ccctgaatat tacgccgtct ctcgtggtag aaaggatgca agcttctccg
     1501 ggtcgatgtt gccgccggag agaaagaagc atatcttctc gccgggtttg accttgaagg
     1561 aaccctgaag cgccgcagcc acgccgaccg aagcggaggg ctcgaccagc agtttgcttt
     1621 tgaagacgat ctccatgagc gccttccgga taaactcgtc cgacgcggga acgatctcgt
     1681 ccacgtactt gtcgatcagc ggataggtat gctctcctgt catcgtgatc atcaggccgt
     1741 cggcgagcgt cgcccccata gggacttcca cgggcttccc cgccttgcgg ctctcggcat
     1801 accgctggat ggattccggt tcaacagcga cgatgcgtac ggaggaacgg gtctccttga
     1861 tggccgtagc cacacccgcg agcagtccgc ccccgccgag cggcacgact acggtgtcga
     1921 cgtccggaag gtcctcgatg atctccagcc cggacgttcc ctgcccggca atcagctcgg
     1981 gatcgttgaa ggagtggacc agcgtgtacc ccttttcggc cttgatctca tagagcttct
     2041 tgtatcgctg tatggagtcg aaaccgtgga gcacgacgtc cgcaccaagc gctctcgttc
     2101 cttctatctt cgatttggga gcgttctccg gaagcacgag cgtcgccttg atgccgagca
     2161 tcttcgccgc ataggcgact ccctgcgcgt ggttccccga ggacgatgcg ataatacctt
     2221 ttgcccgttc ctcgtcggag agggaaagga ttttgttcat cgcgcccctg atcttgaagg
     2281 accctgtaag ctgaaggttc tccggtttga agtagacctc cgcgccgaag agcgcgtcga
     2341 gtttatctcc ccgcagaacc ggcgtccgcc gaactctccc ggagatcctc ccggcagctg
     2401 ttttaatgtc gtcacgtccg atgattcgta ccatattccc tttccgcctc cttgcgcccc
     2461 cgaggggcga aatgcactcc ttcaaaagcc cggatacggc cggaagaagc ttcccgcgcc
     2521 gccccgttct cgtaccgcta ttctattcct ctatccacag cgccatgtcg gggcaggtga
     2581 tctcgcaccg tttacacttg atgcagtctc cctcgcgaac cgcagccacg acggtgaacc
     2641 ccttctggtt cgtctcgttg gatatctcgt agacgttctt cgggcagacc gagatgcaca
     2701 gtccgcaccc tttgcagaga cgcttgttca catgaacggc catcttcact caccccagct
     2761 tccggataat ctccactacc tgggcggggg acgtcgccag atgcgccccc gctttttcca
     2821 aggcttccat tttcgacgcg gcggttccct tgttcccccg gatgatcgcc ccggcgtgtc
     2881 ccaaggactt tccttcggga gcggccctgc cgacgatcaa cgagacgacg ggtttccgca
     2941 cgtactcggc gatgtactcc gccgcggcga tctccgtgcc tccgccgatc tctccgagca
     3001 ggacgatgat ctccgtctcg gggtccgcgt tgaacagctc gaccacctcg cgctggttcg
     3061 tcccccaaat cgggccgccg cccagcgcga cgaccgtgga ctggcctatc cctgcgtccg
     3121 tgagcgtctt gccgactt
//
LOCUS       DAOSWW010000220         3130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22736_length_3130_cov_4.422114, whole genome shotgun sequence.
ACCESSION   DAOSWW010000220 DAOSWW010000000
VERSION     DAOSWW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22736_length_3130_cov_4.422114"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..477)
                     /gene="rpsT"
                     /locus_tag="PK442_05550"
     CDS             complement(196..477)
                     /gene="rpsT"
                     /locus_tag="PK442_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOO87148.1"
                     /translation="MPNKKSAEKRMKTSERNRLYNRFWTTRCKNAVKKVLEAVEGQDE
                     ALAQTRLAIAQSVIDKAVVKGVMHGNTAARRKSMITNRVKALSGEKQPA"
     gene            588..2558
                     /locus_tag="PK442_05555"
     CDS             588..2558
                     /locus_tag="PK442_05555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOO87149.1"
                     /translation="MMTIPSMDEFFGPEGLLNKRFSSFEYRKEQQDLAEEVHKALSAE
                     GEFILAAEAPPGVGKTFALLVPAMLRAAETGETVLVLTAGIPLQEQLIQKDLPLLNEL
                     LGLSLPFGLLKGKGNYACRSRAEEVFEGGEGRQGYLSHRDGGSSSRTVEEWLRTTTTG
                     DLSELSLPPNSPSVAGIAASSRSCRGFRCPRRGECFVQRVLREAREWRVVVANYHLFF
                     SYLLGAGKPFPAPFDLLICDEAHHLPEAARSSMTVSVSDDDVVRLLRSRVFTDTLGRL
                     EKSGRGVQNAAALSAEIRQESARFFELLDVLLPGRKENITVRNEEMLRQQRILSGKML
                     ELYNVFVPLVSDDETPDVSEDGGLSSWMEECGRIRRSLAWCAEVEKYPSWAYWKSERS
                     LLSAPVSPGEELSSGFFTTSAEKMIFISATLALGGKTDFWERETGIHPDRLFISGSPF
                     DLEQQMEILVVDTGLDVMNPRYDDTVCRIVEKLVEANGGSTLVLLASHRLLGKVGRAL
                     SSKPRGYRVLVQGEGPRSELLAAFRSDPSSVLVGSASFREGVDVPGDGLTQVIIDRIP
                     FPHPGDPLVQARNALEGAGAFVRVTLPEAKMLLKQAAGRLIRSRSDRGRVAILDGRVL
                     GRPEWKIPSALPKVKYRRLVVARSAVAPSGGV"
     gene            2631..2765
                     /gene="rpmH"
                     /locus_tag="PK442_05560"
     CDS             2631..2765
                     /gene="rpmH"
                     /locus_tag="PK442_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOO87150.1"
                     /translation="MKQTFQPHNRPRKRKMGFLARSSSPTGRRILRNRRRKGRARLAV
                     "
     gene            2769..>3130
                     /gene="rnpA"
                     /locus_tag="PK442_05565"
     CDS             2769..>3130
                     /gene="rnpA"
                     /locus_tag="PK442_05565"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOO87151.1"
                     /translation="MRFFFPRSSRLRRGWEFDSVFRTGDRLRGELVRLLFLKAEDGVT
                     RIGLAVGKRQGKSHDRNRGRRILKEGFRRILPWIRPGVWIVAGLSGTGMKAGSTDVYE
                     ELARLLKGGGFLEPEWPGA"
BASE COUNT          611 a          837 c         1002 g          680 t
ORIGIN      
        1 ttcggccttc ttgtgctttc gtggaaggaa aagacctctt ttgccgaggg gtggccgggg
       61 ttcgagagcc tactcatgac ttgtatggca tccgcggaga agagacaata aaaaaagccg
      121 aacagcggcg tcgcgcgttc agctttatcg tgttcaaaat ttcaaaggga agaaaaggaa
      181 gagtgtacga aagaactacg cgggctgctt ctccccggaa agcgccttca ctctgttcgt
      241 gatcatggat tttcttcgtg ccgctgtatt gccgtgcata acgcccttta cgactgcctt
      301 gtcgatgacg ctctgggcga tggcaaggcg cgtctgcgcg agagcctcgt cctggccctc
      361 cacagcttcg aggaccttct tcacggcgtt cttgcaccga gtcgtccaga accggttgta
      421 aagcctgttc ctttcgctcg ttttcattct tttttctgcg gatttcttgt taggcatgta
      481 cctcacctcc tcccgataca accacgacat tctagcacgg tttctgttat attagcaaga
      541 gaggcggaga aatttcccgg gaatttcgag tttcagaaag agggtacatg atgacaatcc
      601 catcgatgga cgagtttttc ggccctgaag gtctgttaaa caaaagattt tcgtcattcg
      661 aatacagaaa agagcagcag gatcttgcag aggaagtcca caaagcgctt tccgcggaag
      721 gtgagttcat ccttgcggcg gaggcgccgc cgggggtcgg caagacattc gcgctccttg
      781 ttccggcgat gctccgcgcc gccgaaacgg gggagacggt gctcgtcctc acggcgggca
      841 tccctctcca ggagcagctg atacagaagg accttcccct gctgaacgaa ctcctcggac
      901 tttcgctccc cttcggtctg ctgaagggga aggggaatta cgcctgccgg tcacgggcgg
      961 aggaggtttt cgaaggagga gaaggcaggc aggggtatct ttcgcacagg gacggaggga
     1021 gttcgtcgcg taccgtcgag gagtggctgc ggacgacgac gacgggcgat ctctcggagc
     1081 tctcgcttcc tccgaactct ccgtccgtcg cgggtatcgc cgcgtcgtcg cgctcgtgcc
     1141 gaggattccg ctgtccgagg cggggagagt gctttgtgca gcgggtgctc cgtgaggcgc
     1201 gagagtggcg cgttgtcgtc gccaactacc atctcttttt ctcgtacctc ctcggcgcgg
     1261 gaaagccttt cccggcgccg ttcgacctcc tgatttgcga cgaggcgcat catcttccgg
     1321 aagccgcccg atcctcgatg accgtttcag tgtcggacga cgatgtggtc aggctgctcc
     1381 ggagcagggt tttcaccgat actctcggaa gactggagaa gagcggacgg ggggtacaga
     1441 acgcagcggc gctgtcggcg gagattcggc aggagtcggc acgtttcttc gagcttttgg
     1501 acgttcttct gcccggcagg aaggagaata tcaccgttcg aaacgaggag atgctccggc
     1561 agcagaggat cctctcggga aagatgcttg agctttacaa tgtctttgtt ccgcttgtct
     1621 ccgacgatga aacgcccgat gtgagcgagg acggcggtct ctcctcctgg atggaggagt
     1681 gcgggcgtat caggagaagc ctggcgtggt gcgccgaggt ggagaaatac ccttcgtggg
     1741 cctactggaa gagcgagcgc tccctcctca gcgccccggt gtctccggga gaggaacttt
     1801 cgagcggttt tttcacaacc tccgccgaga agatgatctt catctccgca acgcttgctc
     1861 tggggggaaa gaccgacttt tgggaacggg agacgggaat tcacccggac cgtcttttta
     1921 tcagcggttc tcccttcgac ctcgaacagc agatggagat cctcgtggtc gataccggac
     1981 tcgacgtgat gaatccccgc tacgacgata cggtatgccg catcgttgaa aagctggtgg
     2041 aggcgaacgg ggggagcacg ctggtgctcc tggcgtcgca caggctgctc ggaaaagtcg
     2101 ggcgcgctct gagctcgaaa cccagggggt accgcgtcct tgttcagggg gaaggtccgc
     2161 gcagcgagct tcttgcggca ttccggagcg atccgtcctc cgtgctcgtg gggagcgcct
     2221 ctttccggga gggagtggat gttcccggag acgggttgac ccaggtcatc atcgaccgga
     2281 ttcccttccc tcatccgggg gatcccctgg tgcaggcgag gaatgcgctc gaaggggcgg
     2341 gagcgtttgt tcgcgtcacg cttccggagg cgaagatgct tttgaaacag gccgccggaa
     2401 ggctgatccg aagcagaagc gaccggggcc gcgtcgcaat tctcgacggc cgggtgctcg
     2461 gccgccctga atggaagatc ccgtctgcgc tgccgaaagt gaaatatcgt cgtcttgttg
     2521 ttgcgcgttc cgctgttgct ccaagcgggg gtgtgtgata tagtatcaag gctgaaaaaa
     2581 agccgttgat cagcggctgg ttcttctatt gccgtcagga ggtgtcattg gtgaaacaga
     2641 cgttccaacc ccacaacaga ccacgaaaaa gaaagatggg tttcctcgcc cggtcgagtt
     2701 cgcccacagg ccgtcgtatt ctccggaatc gcaggagaaa gggccgcgcc cggctggccg
     2761 tttaatcctt gagatttttt tttcctcggt cttcacgcct tcgaagaggg tgggagttcg
     2821 atagtgtctt ccgcaccggt gatcggctga gaggcgaact ggtgcggttg ctgtttctga
     2881 aggcggagga tggcgttaca cgcatcggcc tggcggtggg caaacgacag gggaagtccc
     2941 acgaccggaa taggggacgg agaatattaa aagaaggctt ccgcaggatt cttccctgga
     3001 tccggccggg agtatggata gtcgcgggac tcagcggcac gggaatgaaa gcgggctcga
     3061 cggatgtcta cgaagagctg gcccgacttc tcaaaggggg agggtttctg gagccggaat
     3121 ggccgggagc
//
LOCUS       DAOSWW010000221         3123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22818_length_3123_cov_3.058996, whole genome shotgun sequence.
ACCESSION   DAOSWW010000221 DAOSWW010000000
VERSION     DAOSWW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22818_length_3123_cov_3.058996"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PK442_05570"
     CDS             <1..412
                     /locus_tag="PK442_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87152.1"
                     /translation="MYYDGVAVDEDLETALALATDAAEEEEALAQGLLALMYAEGHGV
                     ERDVKSAEGWARRAADQGNEFGWFVLGSLYMRGEVVKKDPAMAWAYLDLAAKRSVANA
                     DERLEELEKTISSRDKKRAQELQKQWRKEWGLSR"
     gene            434..2245
                     /locus_tag="PK442_05575"
     CDS             434..2245
                     /locus_tag="PK442_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOO87153.1"
                     /translation="MSLLSGGMGWRRKGGTKMRAHSRLAAATVAVSMLAGAGGGIASA
                     DAKSSLAESMNLAPRRNADGNYNIIFITTDQERYMQDYPAGTAYEARELLASLGATFE
                     KHYACATMSTSSRSVIFTGKHITDTKMIDNTDFPWQGPLSKEMTTVGDRMRTAGFYSA
                     LKGKWHLGNASTLEDVEEELTDLDEHGFSDWDVDGDYIGTMWEGYNKDEEIVTDTVAW
                     LRNVGAVQNAAGKSFFLAANLINPHDIMYFCVDEEFVGPVPVGGAPDDPLYTKTYDEP
                     LPSSWNRDVSDGTVPAAVSIFKNFMNKQTGVLSTEEEWKETQDYYFNCIQDNDNHLID
                     LLLAVRDMGMLDNSIIFFTSDHGEMGGEHGLKGKGGLLFERNMHVPLIVYHPDYEGGR
                     SVNAVTSHIDLATTFIHMSGLSAERKAEVAEGLPGKNLMELIDGTSQSVRRGALFCNE
                     LISTTMARAVRDVNGAIQYYTFDTTVRGLVRAAITERYKFARYFGGNFNTPTTMEALL
                     RDNDIELYDLENDPGELNNLALDPEANAELIMEMNALLNELIASEIGEDNGDEFHEMI
                     AAYNATLRKESGGCSTASVPAGLLLLLALLPLLRRRK"
     gene            2250..>3123
                     /locus_tag="PK442_05580"
     CDS             2250..>3123
                     /locus_tag="PK442_05580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO87154.1"
                     /translation="MTGNDSRTFAVMAKVVGGFCNLQCEYCYYTEKTKLLAQTRRLMP
                     DDVLEAYVVENLRIHGKDAVVEFAWHGGEPLLAGIDFFRRAMDLERKYGKGRKIVNTV
                     QTNATLLNDEWRQFFAKHDFLLGVSVDGPEALHDAYRRGPDGGSFSRAMAGIELLRKH
                     GIRFNTLTTVNAANEKHPEEVYDFLRRYTDYMQFLPVVESDAAFFEIEDGQRFATPPG
                     IRSMFLQHSKAPFSASAEGYGAFLAGVFDRWKELDYGRKHVQLFETTAGNMRGVPGSL
                     CVHNPLCGHGASVEV"
BASE COUNT          678 a          906 c         1021 g          518 t
ORIGIN      
        1 gatgtactac gacggcgtcg cggtcgacga ggatcttgag acggccctgg cgctggcgac
       61 ggacgcggcc gaggaggaag aggctctggc gcaggggctc ctcgcgctga tgtacgccga
      121 gggacatggg gtggagcgcg atgtgaagag cgccgaaggg tgggcgcgga gggcggcgga
      181 ccaggggaac gagttcggct ggttcgtcct gggatccctg tacatgcgcg gcgaggttgt
      241 gaagaaggac cccgccatgg cgtgggccta cctggacctg gcggcgaagc gctccgtggc
      301 gaacgccgac gaacggctcg aagagctgga aaagaccatc tcctcccgcg acaagaaacg
      361 ggcgcaggag ctgcagaagc agtggcggaa ggagtggggc ctgagtcgct gaacagggcg
      421 cggaggccgg ggcatgtcgt tgctgtccgg cggcatggga tggcgtagaa agggaggaac
      481 gaagatgcgt gcacactcta gattggcggc cgctactgtt gccgtttcca tgctcgcggg
      541 cgcgggaggc ggcatagcct ccgcggatgc gaagtcttcg cttgcggaga gtatgaacct
      601 tgctccgaga cggaacgccg acgggaacta caacatcatc ttcatcacca ccgaccagga
      661 acgctacatg caggactacc ccgcgggtac ggcctacgaa gcgcgggagc tgctggcctc
      721 gctcggcgcc accttcgaga agcactacgc ctgcgccacg atgtccacct cgtcccgttc
      781 cgtcatcttc acaggaaaac acatcaccga tacgaagatg atcgacaaca cggattttcc
      841 gtggcagggc cctctttcca aggagatgac gacagtcggc gacaggatgc gtacggcggg
      901 attctacagc gcgctcaagg ggaagtggca cctggggaac gcaagcacgc ttgaagatgt
      961 ggaggaagag ctgaccgacc ttgacgagca cggattctcc gactgggatg tcgacggcga
     1021 ctatatcggc acgatgtggg aggggtacaa caaggacgaa gagatcgtca ccgatacggt
     1081 cgcatggctt cgcaacgtgg gggccgttca aaatgccgcc gggaagtcct tcttcctcgc
     1141 ggccaacctg atcaatccgc acgacatcat gtacttctgc gtcgacgagg agttcgtcgg
     1201 tcccgttccc gtcggcgggg ctccggacga ccccctgtac acaaagacct acgacgagcc
     1261 gctgccgtcc tcatggaaca gggatgtgag cgacgggacg gttcctgcgg ctgtttccat
     1321 cttcaaaaac ttcatgaaca agcagaccgg tgttctgagc accgaagagg agtggaagga
     1381 gacgcaggac tactacttca actgcatcca ggacaacgac aaccacctga tcgacctcct
     1441 tctggccgtg agggatatgg ggatgctgga caactcgatc atcttcttca cctccgacca
     1501 cggagagatg ggcggagaac atggactgaa agggaagggc ggtctgctct tcgagcgaaa
     1561 catgcacgtc ccgctcatcg tctaccaccc ggactacgag ggagggcgtt ccgtgaacgc
     1621 ggtcaccagc catatcgacc tcgcgacgac cttcatccac atgagcggct tgagcgccga
     1681 acgaaaggcg gaggtcgccg aagggctccc cggaaagaat ctgatggaac tgatcgacgg
     1741 tacgtcgcag tcggtgcgga gaggcgcgct cttctgcaac gagctgatct ccacgacgat
     1801 ggcgcgcgcc gtcagggatg tgaatggcgc tatccaatac tacaccttcg acacgaccgt
     1861 ccgcggcctt gtgcgcgcgg cgatcacgga gcgctacaag tttgcccgct acttcggcgg
     1921 aaacttcaac acgccgacca caatggaagc gctgctgcgc gataacgaca tcgaactgta
     1981 cgatctggag aacgatccgg gcgagctgaa caaccttgct ctcgacccgg aggcgaacgc
     2041 ggagctgatc atggagatga acgcgctgct gaacgagctg atcgcatcgg agataggcga
     2101 ggacaacggc gacgagttcc acgagatgat cgcggcgtac aacgcaacgc tgcgtaaaga
     2161 aagcggcggg tgctccacgg cctccgttcc ggcgggactt ctgctcctgt tggcgctgct
     2221 gccgctcctc cgccgcagga agtaaataga tgacgggaaa cgattcgagg accttcgccg
     2281 tgatggccaa ggtcgtcggc ggtttctgca atctgcaatg cgagtactgc tactacaccg
     2341 aaaagacgaa gctcctcgcg cagacgcgcc gcctgatgcc cgacgacgtg cttgaggcat
     2401 acgtcgtgga aaacctgcgt atccacggga aggacgccgt ggtcgagttc gcctggcacg
     2461 gcggcgagcc ccttcttgcc gggatcgatt tcttcagaag ggcgatggac ctggaacgaa
     2521 agtacggaaa gggcaggaag atcgtcaaca ccgtgcagac caacgcgacg ctgctgaacg
     2581 atgagtggcg ccaattcttc gcgaagcacg actttctgct gggggtgagc gtcgacggcc
     2641 cggaagcgct ccacgacgcc taccggaggg gaccggacgg aggttcgttc agccgcgcga
     2701 tggcgggaat cgaactgctt cggaaacacg gaatacgctt caacacgctg acgaccgtca
     2761 acgcggcgaa cgaaaaacat ccggaggagg tctacgactt cctccgcaga tacaccgact
     2821 acatgcagtt tctgcccgtg gtcgagagcg acgccgcctt tttcgagatc gaagacgggc
     2881 agcggttcgc gacgcctccg gggatcagga gcatgttcct ccaacactcg aaggcgccct
     2941 tttcggcaag tgcggagggg tacggcgcct ttcttgccgg agtcttcgac cggtggaagg
     3001 agctggacta cggacgaaag cacgtccagc tgttcgagac gacggccggc aatatgcgcg
     3061 gcgtccccgg cagtctgtgc gtccacaacc ccctgtgcgg gcacggagcg tccgtcgagg
     3121 tcg
//
LOCUS       DAOSWW010000222         3119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22866_length_3119_cov_2.890339, whole genome shotgun sequence.
ACCESSION   DAOSWW010000222 DAOSWW010000000
VERSION     DAOSWW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22866_length_3119_cov_2.890339"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PK442_05585"
     CDS             <1..672
                     /locus_tag="PK442_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO87155.1"
                     /translation="FAFGTSAEIGPKNVFTYMPQSEETGYLSGLIAGMTTKKNVVGLV
                     GPVDGGDAARYNRGFVLGVHAVNPEAKIMVAHTGSFADFVKAGEVAETQIKAGADVLT
                     GSSQQALGALRAVADHKDAPIWWVGQDIAQIHITEGYKCIAASSYNYAAVIVGLVAKL
                     DAGVRGGECIPMNFSNNGFVFAFNETLKDMYTGAIEQKVNEALEKFRATPETLNWSAV
                     DYSKL"
     gene            740..2278
                     /locus_tag="PK442_05590"
     CDS             740..2278
                     /locus_tag="PK442_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87156.1"
                     /translation="MEPNTRKITSLRVEHITKRFPGVLACNDISLSVAEGEVLALVGE
                     NGAGKTTLMNILMGLYRPDEGRILHGGVRQGVGQRGDAFAAGLGMVHQQYMLVANMTV
                     LENIALGFTQAWSPLKLHLDMVRARIDEVAAKYGLAVDPDAFVWQLSVGEQQRVELVK
                     TLCLGARFLILDEPTSALTPQETDELILLLKRMTSELSIVFISHKLHEVKNLSDKVTI
                     LRHGSVVFEGSTADHSSGDIAALMTGREIHLPRNEAPTCEGNPALEIRGLKVRSDRGF
                     LAVDGLDLSVCAGEIVGLAGVSGNGQRELAEAINGLRKVEGGEILLFGQELANRSPRA
                     VIDAGMGYIPEERNTEGIVPSFSMKENFILKDADEAQFSARSFLRLKEIDRNAEALRT
                     EFDIRSPDTKVAAGSLSGGNIQKVILAREISRKPRFLIAVYPTRGLDMGAAEFIHKRL
                     LEKRLEGIGILLISEELEEILDLSDRVAVIFKGRIIDTLDRRSADSRKIGILMAGVHN
                     DEAV"
     gene            2262..3020
                     /locus_tag="PK442_05595"
     CDS             2262..3020
                     /locus_tag="PK442_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87157.1"
                     /translation="MTKQFKALYKTVVILLAAAAAMLLGALILWTIGADVTKTYSVII
                     LEPLKNKIQIGEVLIRAIPLTIIALGISVAYRSGIINIGAEGQMAMGILAATAVALAL
                     PGLPKYALLPLAMIAGTLGGAAWGFIPGILKAKLHVSELLSTVMLNYIAAQFYTFCLR
                     GPMLDPAEITMGSGTPQSMRLTKAIWLDRLVPGTRIHTGVYIALAMAFIIWLLLWRTS
                     WGYKMRAAGASERAARYGRPGTAASAWRGISSWP"
BASE COUNT          580 a         1043 c          994 g          502 t
ORIGIN      
        1 ttcgccttcg gcaccagcgc cgaaatcggc ccgaagaacg tcttcaccta catgccccag
       61 agcgaggaga ccggctatct ctccggactg atcgcaggca tgacgacgaa aaagaacgtc
      121 gtcggactcg tcggcccggt cgacggcggc gacgcggcgc gctacaaccg cggcttcgtc
      181 ctcggcgtgc atgcggtcaa ccccgaggcg aagatcatgg tcgcccacac cggctcgttc
      241 gcggacttcg tgaaggccgg agaggttgcg gaaacccaga tcaaggcggg cgcggacgtg
      301 ctcaccggct cttcgcagca ggccctcggc gctctgcgcg ccgtcgcgga ccacaaggac
      361 gcccccatct ggtgggtcgg ccaggacatc gcccagatcc acatcaccga ggggtacaag
      421 tgcatcgcgg cgtcctccta caactacgcg gcggtcatcg tcggccttgt cgccaagctt
      481 gacgccggag tacggggcgg cgagtgcatc ccgatgaact tctccaacaa cgggttcgtc
      541 ttcgccttca acgaaacgct gaaggacatg tacacgggcg cgatcgagca gaaggtcaac
      601 gaggccctcg aaaagttccg ggcaaccccg gagacgctca actggagcgc cgtcgactac
      661 tccaagctgt aatccgcatg tatgtcctgc gggggcgccc cctccgcagg actttttttt
      721 ctctcgggag gtctcccgca tggaaccgaa tacccgcaaa ataaccagtc ttcgagtgga
      781 acacatcacc aagcgcttcc ccggagtgct cgcctgcaac gacatctcac tctccgtggc
      841 cgagggcgaa gtgcttgccc tcgtcggaga gaacggcgcc ggaaagacga cgctgatgaa
      901 catcctgatg gggctctacc gccccgacga ggggcgcatc ctgcacggcg gcgtgcggca
      961 gggcgtcggt cagcgcggcg acgccttcgc cgccggactg ggcatggttc accagcagta
     1021 tatgctggta gcgaacatga cggtgctgga gaacatcgcc ctggggttca cgcaggcctg
     1081 gtctcccctg aagctgcacc tcgacatggt gcgcgcccgc atcgacgagg tggcggcgaa
     1141 gtacgggctc gccgtcgacc cggacgcctt cgtctggcag ctttccgtcg gcgaacagca
     1201 gcgcgtggag ctggtgaaga cgctctgcct cggcgcgcgc ttcctcatcc tcgacgagcc
     1261 gaccagcgcc ctcacgccgc aggagacgga cgagctgatc ctcctgctca aacggatgac
     1321 gagcgaactc tccatcgtct tcatcagcca caagctccac gaggtcaaga acctttcgga
     1381 caaggtcacc atcctccggc acggctcggt cgtcttcgag ggcagcaccg ccgaccactc
     1441 ctcgggcgac atcgcggcgc ttatgaccgg acgcgagatt cacctcccca ggaacgaggc
     1501 gccgacgtgc gagggcaatc cggctctcga aatccgcggg ctcaaggtca ggagcgaccg
     1561 cggcttcctc gccgtcgacg ggctcgacct ctcggtctgc gcgggcgaga tcgtcggact
     1621 cgccggggtc tccggcaacg ggcagcgcga gctggccgag gcgatcaacg gcctgcgaaa
     1681 agtcgagggg ggcgaaatcc tcctcttcgg acaggagctt gcgaaccgct ctccacgcgc
     1741 cgtgatcgac gcggggatgg ggtacattcc cgaggagcgc aacaccgagg ggatcgtccc
     1801 ctccttctcc atgaaggaga acttcatcct gaaggacgcc gacgaagcgc agttttcggc
     1861 gcgctcgttc ctcaggctca aggagatcga ccggaacgcc gaagcgctgc gaacggagtt
     1921 cgacatccgc agccccgaca cgaaggtcgc ggcgggctcg ctctcgggag ggaatataca
     1981 gaaggtcatc ctcgcacggg agatctcccg caaaccccgc ttcctcatcg cggtctaccc
     2041 cacgcgcggc ctcgacatgg gggctgcgga gttcatccac aagcgtctcc tggagaaacg
     2101 cctggagggg atcggtattc tgctcatcag cgaggagctg gaggagatcc tcgacctctc
     2161 cgaccgcgtc gccgtcatct tcaaggggcg catcatcgac acgctcgaca gacggtcggc
     2221 ggacagcaga aaaatcggca tcctgatggc aggtgtgcac aatgacgaag cagtttaagg
     2281 cgctctacaa gaccgtcgtc atcctcctcg cggcggccgc ggcgatgctt ttgggcgcgc
     2341 tcatcctctg gaccatcggc gcggacgtga cgaagaccta ctcggtcatc atcctcgaac
     2401 cgctcaagaa caaaattcag atcggcgaag tgctcatccg ggccatcccc ctgacgatca
     2461 tcgcgctggg aatctcggtc gcctaccgga gcggcatcat caacatcggc gccgaggggc
     2521 agatggcgat gggcatcctc gccgcgacgg ccgtggcgct cgccctcccc ggcctgccga
     2581 agtacgcgct gctcccgctg gcaatgatcg cgggaacgct cggcggggcg gcctggggct
     2641 tcattccggg catcctcaag gcgaagctcc acgtcagcga gctcctgtcg acggtcatgc
     2701 tgaactacat cgccgcgcag ttctacacct tctgcctgcg cgggcctatg ctcgaccccg
     2761 ccgagatcac gatggggagc ggcacgccgc agtcgatgcg cctgacgaaa gcgatctggc
     2821 tcgacaggct ggtccccggc acgcgcatcc acaccggggt ctacatcgcg ctggcgatgg
     2881 ccttcatcat ctggctgctg ctctggagga cgtcgtgggg gtacaagatg cgcgccgccg
     2941 gagcgagcga acgggcggcc aggtacgggc ggccaggtac ggcggcatcg gcgtggcggg
     3001 gtatctcgtc gtggccatga tgatcagcgg cgcgttcgcc ggactcgcag gggccgtgga
     3061 ggtcgcgggc gtccaccgcc gcgccatcga aggaatcacc ggcggctacg gcttcagcg
//
LOCUS       DAOSWW010000223         3108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22962_length_3108_cov_3.545365, whole genome shotgun sequence.
ACCESSION   DAOSWW010000223 DAOSWW010000000
VERSION     DAOSWW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22962_length_3108_cov_3.545365"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PK442_05600"
     CDS             complement(<1..697)
                     /locus_tag="PK442_05600"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162312.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOO87158.1"
                     /translation="MNDAHVRIFDTTLRDGEQAAGINLNKEEKLQIAMQLAKMKTDII
                     EAGFPAASPGDFEAVKLIAQQVDGPIIAGLARTRREDILAAAEAVKWSKKPRIHTFIA
                     TSPIHMEFKLKMTPEQVLAEVRQAVTYARSLVEDVEFSAEDASRSDPAFLGEVFRTAV
                     ECGATTLNIPDTVGYSTPDEFGEFVREVIEATGNPVGVIWSVHCHNDLGLAVANSLAA
                     VRAGVRQVECTVNG"
     gene            1177..1464
                     /locus_tag="PK442_05605"
     CDS             1177..1464
                     /locus_tag="PK442_05605"
                     /inference="COORDINATES: protein motif:HMM:NF023159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO87159.1"
                     /translation="MNPEKVNRRTVRRLTDLPNVGPATERDLRLLGVEKPEDLAGRCP
                     FEMYDELCEKTGVRQDPCVIDVFMSVTDFMNGSAPRAWWTFTEERKRKTAR"
     gene            complement(1481..2326)
                     /locus_tag="PK442_05610"
     CDS             complement(1481..2326)
                     /locus_tag="PK442_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO87160.1"
                     /translation="MDAGRFTALIDSFLRSSGWNTVAAGRAINEEMTGLRIFDAPLTG
                     VASADDPAFRDLRRESVVGPHFLLPEEWLPGARAVVSFFLPFSENVRRANRADRDEPA
                     PEWLHGRIEGQECIDRLSAFLVERLLEEGFDAVAPSVDERFRSCTTTNTVFLDGDGAP
                     LPVSFNSNWSERHVAYVCGLGSFGLSKGLITPKGVAGRFGSVVTSAPIPPDNVVASAV
                     YENCTMCGACVRNCPVGAITIEKGKDHVRCAEFLTLTKEKYRPRYGCGKCQTGVPCER
                     GIPPR"
     gene            2636..>3108
                     /locus_tag="PK442_05615"
     CDS             2636..>3108
                     /locus_tag="PK442_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO87161.1"
                     /translation="MAMNAEYTPLFRARGITKIYHPGTETEVRALDGVDLDLYEGELA
                     VLLGASGSGKSTLLNILGGLDVPTDGTLSYRDEELTGAGERELTLFRRNVAGFVFQFY
                     NLIPSLTARQNVALITEIAPDPMEPEEALGLVGLSDRMDHFPSQLSGGQQQRVAIA"
BASE COUNT          590 a          963 c          941 g          614 t
ORIGIN      
        1 gtccgttgac agtgcactcc acctgtctga ctccggctct gacggccgcg agcgagttcg
       61 ccaccgcgag tccgaggtcg ttgtggcagt ggaccgacca gatcacgccc acggggttcc
      121 cggtggcctc gatgacctcg cggacgaact cgccgaactc gtccggagtc gagtagccta
      181 cggtgtcggg gatgttgagc gtcgtcgcgc cgcactcgac cgccgtccgg aagacctctc
      241 ccagaaaagc gggatccgag cggcttgcgt cctccgcgga gaactcgacg tcctcgacga
      301 gactgcgcgc gtacgtcacg gcctgccgca cctcggcgag cacctgctcc ggggtcatct
      361 tgagtttgaa ctccatgtgg atggggcttg tggcgatgaa cgtgtggatg cgaggtttct
      421 tcgaccactt gaccgcctcg gcggctgcga ggatatcttc ccgccgcgtc cgggcaagtc
      481 cggcgataat cgggccgtcc acctgctgcg cgatgagctt gaccgcctcg aagtcgccgg
      541 gggaagcggc ggggaacccc gcttcgatga tgtccgtctt cattttcgcc agctgcatag
      601 cgatctggag tttctcctcc ttgttcaggt tgatccccgc cgcctgctct ccgtctctca
      661 gggtggtatc gaaaatccgt acgtgtgcgt cgttcataat tctctctcct ttcaaaatca
      721 tccgtgaaat aaaagtacaa aaaaaggccg gaacctctaa aaaagaggtt ccggccttgg
      781 aatgccgtta cgcagcttac cgggctgccg gcgttccagg cgggaaccgg caaccgagga
      841 ggaggcacag ggaggaagcg ttcatgatgc gtgaacgtac ttctttagcg ccagttgcat
      901 gcgctataag tgatcgacac agctttttac tcgcttcgct cgtgttcgcc ggtcgtctac
      961 ttaacataac tgattccctc ctctcctcat ggctcggtaa gattggtaca ttttgaggga
     1021 gtacccctgg aatgtcaata ctcaaacaaa agaaaaacaa aaggcgttta ttctgataaa
     1081 aggctataac tttgaccggg tcgcgtggaa aatcccggcg tggtagaatg cttcgcaaag
     1141 ggcgctccgg cgccctttcg aggagaggtg cgtgcgatga accccgaaaa agtgaatcgt
     1201 cggacggtcc ggcggctgac cgatctgccc aacgtcggac cggccacgga aagagatctc
     1261 cgtcttctcg gcgttgagaa gccggaagac cttgccggtc gctgtccgtt cgagatgtac
     1321 gacgagctct gcgagaagac gggagtccgg caggacccct gcgtgatcga cgtctttatg
     1381 tcggtgaccg acttcatgaa cggttccgct ccgcgcgcgt ggtggacgtt cacggaggag
     1441 cggaagcgga aaacggcccg ctgacgcggc ccgcgcggga ttacctcggc ggtatcccgc
     1501 gctcgcacgg gacgccggtc tggcacttcc cgcacccgta tcgcgggcgg tatttttctt
     1561 tcgtcagagt taaaaactcc gcgcagcgga cgtgatcctt ccccttttcg atggtgatgg
     1621 cgccgaccgg acagtttcgg acgcacgcgc cgcacatggt gcagttttca tacacggccg
     1681 aggcgaccac gttgtccgga ggaatcggcg cgctcgtgac cacgctgccg aatctccccg
     1741 cgacaccttt cggcgtgatc agccccttgg agagcccgaa cgaccccagt ccgcagacat
     1801 aggcgacatg ccgctccgac cagttgctgt tgaacgatac cggcagagga gcgccgtcgc
     1861 cgtccagaaa caccgtgttc gtcgttgtgc aggagcggaa gcgttcgtcg acggacggag
     1921 cgaccgcgtc gaatccttct tccagaagac gctcgacaag gaacgcggaa agacggtcga
     1981 tgcactcctg cccttcgatc ctgccgtgca gccactcggg ggccggttcg tccctgtcgg
     2041 cgcggtttgc gcggcgcacg ttctccgaga acgggagaaa gaacgaaacc acggcgcgcg
     2101 cgccgggaag ccactcctcc ggcaaaagga agtgcggccc cacaacgctc tccctgcgaa
     2161 gatcccggaa ggcggggtca tccgccgagg cgacgcccgt aagcggcgcg tcgaagatcc
     2221 ggagtccggt catctcttcg ttgatggcgc gcccggctgc gaccgtgttc caaccggaac
     2281 tccgcagaaa actgtcgatc agcgcagtaa accgtcctgc atccatagag atctccgtcc
     2341 tttccatatc acggcgggga gcttttaata caacgcgctc accgcctcgt tcaccgcggg
     2401 aatccgcgtc ttccctccct cgtttgcagc ctctatacta cgtgaaaagg ccctgttttc
     2461 caagatctgt cggtccatgc cagtcttttt ccgcaaagcg tccgatgaaa aatctgcgga
     2521 aatcttccgc tattgtttga ctgatgcagc ccattgggat ataacgttgc aggaacttcc
     2581 cgctgccgat actgcgtgca gggggtgcag cgcgtgtttt tcaggaggaa cgttgatggc
     2641 gatgaatgcg gagtacaccc ccctctttcg agcgcggggg atcacgaaga tttaccatcc
     2701 ggggacggag acggaggtgc gcgccctcga cggcgtcgat ctcgatctct acgaggggga
     2761 gctggccgtg ctcctcggcg cctcggggag cgggaagtcc acgctgctca acatcctcgg
     2821 cgggctggac gtcccgacgg acgggacgct ctcgtacagg gacgaggagc tcaccggcgc
     2881 gggcgagcgg gagctcacgc tcttccgcag gaatgtcgcg gggttcgtct tccagttcta
     2941 caacctcatt ccgagcctca ccgcgcgcca gaacgtggcg ctcatcaccg agatcgcgcc
     3001 cgatcccatg gagccggaag aggcgctcgg tctcgtcggt ctttcggaca ggatggacca
     3061 cttcccctcg caactctccg gagggcagca gcagcgcgtc gccatcgc
//
LOCUS       DAOSWW010000224         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_22989_length_3106_cov_3.340872, whole genome shotgun sequence.
ACCESSION   DAOSWW010000224 DAOSWW010000000
VERSION     DAOSWW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22989_length_3106_cov_3.340872"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK442_05620"
     CDS             complement(<1..198)
                     /locus_tag="PK442_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87162.1"
                     /translation="MSTENSERNKQPWSQNIPRTVLVFAPRILGIGFILFMSLFALDV
                     FSEDADAWSMCLGFMIHMIPSA"
     gene            complement(223..1014)
                     /locus_tag="PK442_05625"
     CDS             complement(223..1014)
                     /locus_tag="PK442_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOO87163.1"
                     /translation="MDGYSRAVRLWQSYGIASAADLDKYLHSFRILFAYHSGKIENEE
                     ITYHDTREIFENGRVTGYTGSPRALFEQQNQKLCYEFLKEKIVSLEPLGMELVKEVHR
                     LLTAGTYDERRYIDNGERPGEFKKHDYVTGVHEVGSSAEDVEKEVAELIAEIDAYEGD
                     DVLKAAAYFHARFEYIHPFADGNGRVGRTLLNYYLMIRGHPTLIVHDEEKGRYYAALQ
                     TYDEAEDLAPLYEFFRYETEKTWAKALTLLDGTKPDRKGLSDLLN"
     gene            complement(1017..1499)
                     /locus_tag="PK442_05630"
     CDS             complement(1017..1499)
                     /locus_tag="PK442_05630"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO87164.1"
                     /translation="MEHVIEECGDPRSIPRDLLYLADPSDEMLNAYVDVSYAFVCRID
                     REIVGALLMMPTRPFTMEIMNISVREPYWNQGIGRKLIQGAVKKARETGMKKVEIGTG
                     NPGVVQMLLYQKCGFRITGVEFDYFRKRCAEKIIENGIECRDMIRMEMHLDEETQKEG
                     "
     gene            complement(1563..2891)
                     /locus_tag="PK442_05635"
     CDS             complement(1563..2891)
                     /locus_tag="PK442_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87165.1"
                     /translation="MAMYRAAIEKLKRWKAKEAPKPLIIRGARQVGKTWLMKEFGRSA
                     YENTVYIGFENNRQMKELFSGDMAIDRIVTGLELYAGRKIDPENTLLVFDEIQEVPAA
                     LTALKYFNENAPQYRIVCAGSLLGVALHPGTSFPVGKVEFLDLFPLSFSEFMTAMGKE
                     RFAELLQKGDFELATTFKYEFIDLLKHYYYVGGMPEAVASFAGNRDFNEAREIQRRIL
                     AAYEQDFSKHAPNEIVPRIRMLWNSVPAQLTKENKKFVYGVVKEGARAKEYEMAMLWL
                     ADCGLVYKVHRATAPRLPLKAYEDLKAFKLFLLDVGLLSCMVRLDQRLLLEGSALFTE
                     FKGALTEQYVLQQLKTIEDVDTYYWTNERGGAEIDFLVDAGDEVVPVEVKAETNLKAK
                     SLKTFCEKFRPKSAVRVSMADYKREERLLNLPLYAVETIGNGGVRPEGGK"
BASE COUNT          625 a          916 c          782 g          783 t
ORIGIN      
        1 cgcggagggg atcatatgaa tcataaatcc caaacacatc gaccatgcat cggcatcttc
       61 agaaaagaca tcaagagcaa agaggctcat gaataaaata aatccaatcc cgagtatccg
      121 cggagcaaac acaagcacgg tccgcggtat attttgagac caaggctgtt tgttacgctc
      181 cgaattttcc gtcgacatgg ctgtattctc ctttccgcaa tcctaattta ataaatccga
      241 cagacctttg cgatcgggct ttgtgccgtc aagcaaggtc aaggctttcg cccacgtttt
      301 ttcagtctca tacctgaaaa actcgtacag aggggccaaa tcctccgctt cgtcgtacgt
      361 ttgcagggcc gcgtaatatc ttcctttctc ctcgtcgtgg acgataaggg tgggatggcc
      421 gcggatcatg aggtagtagt tcagaagggt tctgccgacg cgtccgttgc cgtcggcgaa
      481 ggggtggata tattcgaacc tcgcgtgaaa ataggcggcc gccttgagga cgtcatcgcc
      541 ctcgtaggcg tctatctcgg cgatcagctc cgcgacttct ttttccacat cttcggcgga
      601 cgacccgacc tcatgaactc cggttacgta atcgtgcttt ttgaattcac ccggccgctc
      661 accgttatcg atatacctgc gttcgtcgta tgttccggcg gttagcaagc gatgcacttc
      721 cttcaccagt tccataccga gcggttcgag gctcacgatt ttctctttca gaaattcgta
      781 gcacagcttt tgattctgtt gctcgaagag agcgcggggg cttccggtat agcccgtcac
      841 tctgccgttt tcgaagatct cccgcgtgtc gtgataggtg atctcctcgt tctctatctt
      901 tccggaatgg tacgcgaaca gaatgcggaa gctgtggagg tatttatcca aatcggcggc
      961 cgacgcgatt ccgtacgact gccacaggcg cactgcacgg ctgtatccgt ccatattcat
     1021 ccctcttttt gcgtctcttc gtccaggtgc atctccatcc tgatcatatc ccggcactct
     1081 atcccgtttt cgataatctt ttctgcacag cgctttctga agtaatcgaa ttcgacgccc
     1141 gtaattcgaa agccgcactt ctggtagagc aacatctgga cgacgccggg atttcccgtc
     1201 cctatctcga ctttcttcat gcccgtctcg cgcgctttct ttacggcccc ttgaatcagc
     1261 tttcttccta tcccttgatt ccagtacggc tcgcgcaccg atatattcat gatctccatc
     1321 gtgaacgggc gcgtcggcat catcagcagc gcgccgacga tctcgcgatc gattctgcac
     1381 acgaacgcgt aacttacgtc cacataggcg ttcagcattt catcggacgg gtcggcgaga
     1441 tagagcagat cccgcggaat gctccgggga tcgccgcact cttcgattac gtgctccact
     1501 ttcgatacac ctctctttca tacatggtcg agccacttcg atccggcccg acaaccgcaa
     1561 ttctacttcc caccttccgg gcgcacacca ccgtttccga tcgtttccac ggcgtagagt
     1621 ggcagattga gcagccgctc ctctcttttg taatcggcca tggaaacgcg gacggcgctc
     1681 ttcgggcgga atttttcaca aaaggtcttc aggcttttcg ccttgaggtt cgtctcggcc
     1741 ttgacttcca cggggacgac ttcgtcgccg gcgtccacga ggaagtcgat ctccgcgccg
     1801 cctcgttcgt tggtccagta gtatgtgtcc acatcttcga tcgttttcaa ctgctgcaga
     1861 acgtactgtt cggtcaacgc gcccttgaac tccgtgaaaa gagcgctgcc ttccaacagt
     1921 agccgttgat caagtcgtac catgcacgaa agaaggccga cgtcgagcag aaaaagcttg
     1981 aacgccttca ggtcttcgta ggctttcagc ggaaggcggg gggccgtcgc acgatgaacc
     2041 ttgtagacca gtccgcagtc ggcgagccag agcatggcca tttcgtactc cttggccctc
     2101 gctccctcct tgacgacacc gtagacgaat tttttgttct ccttcgtcag ttgcgccggg
     2161 acgctgttcc agagcatgcg gattcggggc acgatttcat tcggcgcgtg cttcgagaaa
     2221 tcctgttcat aggcggccag aatgcgccgc tgtatttcgc gtgcctcgtt gaagtcgcga
     2281 tttccggcga aactcgccac ggcttcgggc attcccccga cgtagtaata gtgcttcagc
     2341 aggtcgataa actcgtactt gaaggtggtg gccagctcga agtcgccctt ttgcagaagc
     2401 tccgcgaatc gctccttccc catcgccgtc ataaactcgc tgaaagagag cggaaagagg
     2461 tcgagaaact ccacctttcc gacgggaaag gacgtccccg gatgcaacgc gacgccgagc
     2521 agcgagcccg cgcagacgat tctgtactgc ggggcgtttt cgttgaagta cttgagggcg
     2581 gtcagggctg cgggcacttc ctgaatctcg tcgaagacga gcagcgtatt ctccggatcg
     2641 atctttcgtc cggcgtacag ctccagcccc gtgacgatac ggtcgatcgc catatcgccc
     2701 gagaacagct ctttcatctg gcggttgttt tcgaatccga tgtagactgt gttttcgtag
     2761 gcgctccttc caaactcctt catcagccag gtcttgccga cctgacgcgc tcccctgatg
     2821 atgagcggtt tcggcgcttc tttcgccttc catcgtttca gcttttcgat cgcggcacgg
     2881 tacatagcca tcacctcgcg attgtgatta tacacccgac gcacatattt tacgacgaat
     2941 acgcgggcgt ataacacata ttttacggtg aaaatcgaag cgacgaaagg gctattccac
     3001 tacgggagct ctcttctctt caagcgtttc cattcgttcg ttcacgcgcg aacctcctcg
     3061 gttttcttcg cgaggcccta ctcggggcgg cgggcgacga aaaatt
//
LOCUS       DAOSWW010000225         3102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23031_length_3102_cov_3.625533, whole genome shotgun sequence.
ACCESSION   DAOSWW010000225 DAOSWW010000000
VERSION     DAOSWW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23031_length_3102_cov_3.625533"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="PK442_05640"
     CDS             complement(<1..1668)
                     /locus_tag="PK442_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOO87166.1"
                     /translation="MKVPPVAGTRFFPVNRGEMEERGWAELDFLFVSGDAYVDHPAFG
                     TAVIARVLEAAGFRVGILAQPDWRSRDAFLSMGRPRYAALVSAGNLDSMLNKLTASKK
                     SRSTDSYSPGGQAGMRPDRATIAYCSRVKECWKDLPVIIGGVEASLRRFAHYDYWSDE
                     VRRSILFDSQADLLVYGMGELQIRQIADLLSRGVPVGRLQDLRGTVYRTNEFPEIRGK
                     FVEIPSYEDVLSDKKRFAEAFRMQDAEQDAFHGKTVVQRHGASFIVQNPPSPPLSTEE
                     MDAVYALPYTRTYHPMYEAAGGVPAIAEVRFSLTSHRGCFGSCSFCAIHYHQGRIIQS
                     RSHDSLLREARILTELPDFKGYIHDVGGPTANFRIPACDQQLVRGACRNRQCLSPTPC
                     PKLRADHSDYIDLLRKLRALPKVKKVFIRSGIRFDYLMADKKGNFLEELCAHHVSGQL
                     KVAPEHVSSRVLALMGKPGCEVYEKFRLAYAEMNEKLGKKQYLVPYFIASHPGATLED
                     AVELAEYLRDIHFQPEQVQDFIPTPGSLSTCMYYTGLNPFTGEAVPSA"
     gene            complement(1683..>3102)
                     /locus_tag="PK442_05645"
     CDS             complement(1683..>3102)
                     /locus_tag="PK442_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87167.1"
                     /translation="IPGKEERGGDRLCPECGSRVENSFRFCPGCGSFVPEYASVRQVP
                     GAQRKETEYEDDSEDDGVVSVACGPGGNEREVEGAFAYEQPRSNADRAGTSFREFNAA
                     IQDRRGRRDRKGGIFLLLLFFMFVAALGGGVYWFLQQAEQIPWENDVREKPAADPTPV
                     AQENRDPDLPPLATPGGAPADVTPALPLSPPVQADALEISRPTKGVVIGSGVNLRGSH
                     TVSSPVVGKVTTGNEVEVLESWTSDEGAEVVALVNVELVADDGKTAKIVRGRGLSVIS
                     GPDSQGMLRVALPNDRSKKQYSVAANTMSNPHSWPWYKIRHGGGKEGWIFGKFITVFN
                     PRENTLSPTVLDRALHTFGSTKEALEGALGKPRKTASRKVTRSGVAADELTLTFNGVT
                     AVLQERGGASEVKSLTLSSSKHSLDGGFAVGSDRLAVLSILGLPNALDKGSEVYRLDA
                     NSGIRIHYESYKVRTIHVGMLK"
BASE COUNT          567 a         1006 c          952 g          577 t
ORIGIN      
        1 cgccgacggc acggcctccc ccgtgaacgg gttcagcccc gtgtagtaca tgcaggtcga
       61 caggctcccc ggcgtgggaa tgaagtcctg cacctgctcg ggctggaagt gaatgtcgcg
      121 cagatactcc gccagctcca cagcgtcctc aagcgtcgct ccggggtggg aggcgatgaa
      181 gtacggcacg aggtactgct tcttccccag cttctcgttc atctcggcat aggccagacg
      241 gaacttctcg tacacctcgc atccgggctt ccccatcagg gccagcacgc gggacgaaac
      301 gtgctcgggc gccaccttca actgcccgct cacgtggtgc gcgcacagct cctcaaggaa
      361 attgcccttc ttgtccgcca tgagatagtc aaagcgaatg ccggagcgga tgaagacctt
      421 cttcaccttc ggaagcgcgc gcaacttacg gagcaggtcg atgtagtccg agtggtcggc
      481 gcggagtttc gggcaggggg tcggggagag gcactgcctg tttctgcacg cccccctgac
      541 gagctgctgg tcgcaggcgg ggatgcggaa gttcgcagtc gggccgccga cgtcgtggat
      601 gtaccccttg aagtcgggca gctcggtcag aatgcgcgcc tcccgaagca gggagtcatg
      661 gctgcggctc tggatgatcc gcccctggtg gtagtggatg gcgcagaagg aacagcttcc
      721 gaagcagcct cgatggctgg tcaggctgaa gcgcacctcg gcgatcgcgg gaacgccccc
      781 cgccgcttcg tacatcgggt ggtaggtccg tgtgtacgga agcgcgtaga cagcgtccat
      841 ctcctccgtc gaaagcggcg gcgagggagg gttctgtact ataaacgacg cgccgtgacg
      901 ctgcaccaca gtcttgccgt ggaacgcgtc ctgctcggcg tcctgcatgc ggaaggcctc
      961 ggcgaaacgt ttcttgtcgg agagaacatc ttcgtaggag gggatctcca cgaacttgcc
     1021 gcggatttca gggaactcgt tcgtcctgta gacggttccg cgaagatcct ggagccgtcc
     1081 gacagggacg cccctggaga gcaggtcggc gatctgtcgt atctgaagct cccccatccc
     1141 gtagaccagc agatccgcct ggctgtcgaa gagaatggaa cgccgcacct cgtcggacca
     1201 gtagtcgtaa tgggcgaacc gccgaaggct cgcctctacg ccgccgataa tcaccggaag
     1261 gtctttccag cactccttca ccctgctgca gtaggcgatc gtcgccctgt ccgggcgcat
     1321 ccccgcctga ccgccgggag agtagctgtc ggtactccgg ctcttcttcg aggccgtcag
     1381 tttgttgagc atcgagtcaa gattgcctgc ggagacgagc gccgcatagc gcggtctccc
     1441 catggagagg aacgcgtccc gggagcgcca gtcgggctgc gccagaatac ccacgcgaaa
     1501 acccgcggcc tccagcacgc gggcgatcac cgcagtaccg aaggccggat ggtcgacata
     1561 ggcgtcgccg ctgacgaaga ggaaatccag ctcggcccag ccgcgttcct ccatctcccc
     1621 cctgttcacg gggaagaagc gcgtcccggc aacaggggga accttcatcg ggggaaaagc
     1681 gttcacttca gcataccaac atgaatcgtc ctgaccttgt agctttcgta gtggatacgg
     1741 atgccgctgt tcgcgtcgag acgatagact tcgctcccct tgtcgagggc gttcggaagc
     1801 cccagaatcg agaggacggc caagcggtcg ctcccaacgg cgaatccgcc gtcgagagag
     1861 tgtttcgagg aggagagcgt gagcgatttg acttccgacg cacccccccg ctcctgcaac
     1921 accgcggtca cgccgttgaa ggtcagcgtg agttcgtccg ccgcgacgcc cgagcgagtg
     1981 accttcctcg aggcggtttt ccgcggttta ccgagcgcgc cttcaagcgc ttcctttgtg
     2041 gaaccgaaag tatgcagcgc acggtccaga actgtcggcg acagggtatt ctcgcggggg
     2101 ttgaagacag tgatgaactt gccgaaaatc cacccctcct tgcctccgcc gtggcgaatc
     2161 ttgtaccagg gccatgaatg cgggttgctc atcgtattcg cggccaccga gtactgtttc
     2221 ttcgaacggt cgttgggcag cgccacacgg agcattccct gcgaatccgg cccggaaatc
     2281 acggaaagcc cgcgcccccg gacgattttt gctgtcttgc cgtcgtccgc gacaagctcg
     2341 acgttgacca gggcgacgac ttccgccccc tcgtcgctcg tccacgactc cagtacctcg
     2401 acctcgttgc ccgtggtcac cttccccacg acggggctgc tcaccgtgtg cgagccgcga
     2461 aggttcacac cggagccgat gactaccccc ttcgtcggac gggagatttc gagggcatcc
     2521 gcctgcacgg ggggggacaa gggcagtgcg ggagtcacat cggcgggcgc ccctccgggc
     2581 gtcgccaacg gcggcagatc aggatcgcgg ttctcctgcg ccacaggcgt cgggtcagcc
     2641 gcgggctttt cacgcacgtc gttctcccag ggaatctgct ccgcctgctg aaggaaccag
     2701 tagacgcctc cgccgagcgc cgccacgaac ataaaaaaga ggagaagcag gaaaatgccg
     2761 cccttcctgt ctcttctgcc tcggcgatcc tgaatggctg cgttgaactc acgaaaactt
     2821 gtgcctgccc tgtcggcgtt ggaacgaggc tgctcgtacg caaacgcccc ctcgacctcc
     2881 ctctcgtttc cgcccggtcc acacgctacg gagacgacgc catcatcttc actatcgtct
     2941 tcgtactccg tttctttgcg ctgtgcaccg ggaacctggc gtacagaggc gtattcaggg
     3001 acgaacgagc cgcatccggg gcagaagcga aagctgttct ccactctgct cccgcattcc
     3061 ggacaaaggc ggtcgccgcc ccgttcttct tttccgggaa tc
//
LOCUS       DAOSWW010000226         3102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23034_length_3102_cov_2.979652, whole genome shotgun sequence.
ACCESSION   DAOSWW010000226 DAOSWW010000000
VERSION     DAOSWW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23034_length_3102_cov_2.979652"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PK442_05650"
     CDS             <1..490
                     /locus_tag="PK442_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87168.1"
                     /translation="VVLGIRDGIALAGFLSPESLEKAPELSQGTEKLLGGNNSMLFYL
                     DADVVRRIVVQIFDPENPIAALFLADDDDFLEAAPFLFEGLKATAGFKGIQMNFSGME
                     RLDFAMLFDTPNSEIIAALDGLAAKWQEFMETQEEEEEAEEEEAEEEEKIEEEAKEAP
                     KS"
     gene            570..2525
                     /locus_tag="PK442_05655"
     CDS             570..2525
                     /locus_tag="PK442_05655"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOO87169.1"
                     /translation="MRKCLWILVFACLVWGFAAAPLFAEERILRFDVEATVEEGASLI
                     VTERIAFVAEGNDIKRGLYRTFPTDFTDTEGVRRRAGFDLLSAELDGAPTPSLVERVG
                     ENLDIRLGDLEKLLAHGEHQFTITYRTSGQLGFFGEHDELYWNVTGNEWAFPIEKVSF
                     RLRLPGRDFGADFSSIEFYTGKKGERGQDAFVTKEGTVESTRLLSQGEGLTVVYTWPK
                     GMVAPPAEPAPVLEKWTPSPYRVAHLAMPVVLALVMTLLWILWGKDPPAKAVFPRFAP
                     PQGIEAGFSRYVWSMRMDDQAFAAMVLGMAVKGPLTIEERSLVAEAAKQTGKDASQAS
                     MGMKLLSKLVGKSYVLRLNREKLSNTNLTIDERVLVDELFGSTRSDIHLSSADRPVIQ
                     DAFGQLGKRFKERAKPLLKTNTGKWLAGVALFEIYAVVMLVLMILSGEGRFEPVLALM
                     AGPFLLLPFAIPVPSGKGSMMSKFFLRVFFPGIFLFVTAGAVLAGSTSGIEVDLLSVP
                     GPFLCVLMLLLFKPLMRVRSEEGARLNEELEGLRMFMTATEKHRLELENPPEETPKLF
                     EELLPYAFALDVAETWANRFENILAAAHYTPSWYRGDMHTFSQTAGVAAFASGFAGAV
                     ASGTRSSGRGGSGSSGGGGGGGGGRGW"
     gene            2559..>3102
                     /locus_tag="PK442_05660"
     CDS             2559..>3102
                     /locus_tag="PK442_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOO87170.1"
                     /translation="MRVVKICAVLASILLLSVPLAANPTVESFEWPKSGMLVGERVNF
                     RSGPATSSKVIGRFVDENEGGELLVTGRSEGREEFPWYRVVSHKFGEGWLYGKFIAVE
                     EPEDPVRRYALKIREDFGLSPAQAVKMYGEPMKRSREKLHIPDFNVTAEIVTLEFHGH
                     TAIYWDGVLQSVEIPAGFMGF"
BASE COUNT          675 a          780 c          933 g          714 t
ORIGIN      
        1 cgttgtcctt ggcattcgcg acggtatcgc gcttgccgga ttcctcagtc cggaaagcct
       61 ggagaaggcg ccggaattaa gtcagggtac agagaagctt ctcggcggaa acaattctat
      121 gcttttctac ttggatgcag acgttgttcg ccgtatcgtg gtgcagattt tcgatccgga
      181 gaaccctatc gccgcgctct tcctcgctga cgacgacgac ttcctggagg ccgctccgtt
      241 tctcttcgag ggattgaagg ctacagccgg attcaagggt atccaaatga acttcagcgg
      301 aatggagcgt cttgatttcg cgatgctctt cgatacgccg aattctgaga taatagccgc
      361 tctcgacgga cttgccgcaa aatggcagga gtttatggag actcaagaag aggaagaaga
      421 agcagaagag gaagaagcag aagaagagga aaagatagaa gaggaagcga aggaagcgcc
      481 gaagtcctag ttcacgaatt tcaagaagct ccggggatat ttgacctcgg agcttctttt
      541 tcctctgcca tgcaaggagg tggaaggcca tgagaaaatg tttatggata cttgtattcg
      601 cgtgtctggt atggggtttt gcagcagcgc cgctttttgc agaggagcga atactccgtt
      661 tcgacgtcga agcaacggtg gaagagggag cctcccttat cgtaacagag cgcatcgcgt
      721 ttgtggcgga gggaaacgac ataaagcgtg gactctacag aactttcccg actgatttta
      781 ccgatacgga aggcgttcgc agaagggctg gttttgatct tctttccgcg gagcttgacg
      841 gggcgccgac cccatcgttg gtagaacgtg ttggagaaaa cctcgatatt cgcctcggcg
      901 acctcgaaaa actccttgct cacggcgagc atcagtttac aataacatac aggacatcgg
      961 gacagctcgg ttttttcggg gaacacgacg aactgtactg gaacgtcaca ggaaatgaat
     1021 gggcgttccc gatagaaaag gtatcatttc gtctacgact ccccggaagg gatttcggcg
     1081 ctgatttttc gagcatagag ttctataccg gaaaaaaggg agagcggggg caggatgcat
     1141 tcgtaacgaa agaagggacg gtggagtcta ctcggttgct ttcgcaaggc gagggcttga
     1201 cagtagtcta cacatggccg aagggcatgg tcgcccctcc ggctgaaccc gcgcccgttc
     1261 tggaaaaatg gacgccttcc ccgtacagag tagcgcacct tgcaatgccc gttgttcttg
     1321 ccctggtgat gacgctgctc tggatactgt ggggcaaaga tccccccgca aaagcagtat
     1381 tcccccggtt tgcccctcct cagggaatcg aggccggttt ttcccgctac gtatggtcga
     1441 tgcgcatgga cgaccaggcg ttcgccgcaa tggttctggg gatggccgtc aagggaccct
     1501 tgacgatcga ggagcgttct cttgttgcgg aggcggcgaa gcagacggga aaagatgctt
     1561 cgcaggccag tatgggtatg aagctgcttt caaagcttgt cggaaaatcc tatgttcttc
     1621 gcctgaacag ggagaagctc tcgaatacca acctcaccat cgatgaacgg gttcttgtcg
     1681 acgagctttt cggctccacc cgaagcgaca tccatctctc aagcgcggac cgtcctgtaa
     1741 ttcaagatgc tttcggacag cttgggaaac gcttcaagga gagggcaaaa ccgcttctga
     1801 agaccaacac cggaaagtgg ctcgctggag tcgctctttt cgagatctac gccgtcgtca
     1861 tgcttgttct catgatcctc tccggcgagg ggcgttttga acctgtgctt gctctgatgg
     1921 ccggaccgtt tctcctgctt cctttcgcca ttccagttcc gtcgggaaag ggaagtatga
     1981 tgtcgaaatt tttcctcaga gtttttttcc ccggcatctt cctcttcgtt acggcagggg
     2041 ctgtgctggc gggttcgacc agcgggatcg aggtcgacct cctctcggtt cccggtccgt
     2101 tcctgtgtgt gctgatgctg cttctcttca aacccctgat gcgcgtccgc tccgaggaag
     2161 gcgccaggct gaacgaggag cttgaaggtc ttcgcatgtt catgacggca acagagaaac
     2221 accggcttga attggaaaac cctcctgaag agacgccgaa gctctttgag gagcttcttc
     2281 cctacgcctt tgccctcgac gtcgccgaga cgtgggcgaa ccgttttgag aacattcttg
     2341 ccgccgcgca ctatacgccg tcgtggtaca gaggggatat gcataccttc tcacagacgg
     2401 ccggcgtcgc cgcgttcgct tcaggttttg caggcgcagt cgcgtccgga acgcgctcct
     2461 cggggagagg cggaagcggc tcctcgggag gaggaggcgg cggaggcgga ggacggggct
     2521 ggtagtattc tttctttatc gaaaggaggg aacggataat gagagtagta aaaatctgcg
     2581 cggttttggc gagtatcctc cttctctctg ttccgttggc cgcaaatccg acggtggaga
     2641 gcttcgaatg gccaaagtcg ggcatgctgg ttggggagag ggtgaatttt cgttcagggc
     2701 cggcaacttc atcgaaggtc atcggccgtt tcgtggacga aaacgaaggg ggcgaactct
     2761 tggtgacggg gagatccgaa ggtcgcgaag agttcccgtg gtatcgtgta gtctctcaca
     2821 aattcggaga agggtggttg tatggcaaat tcattgccgt cgaggaaccc gaggatccgg
     2881 tgcgccgtta cgcgctgaaa atccgcgagg acttcgggct ctctcctgct caagccgtga
     2941 agatgtacgg cgaaccgatg aagcgttctc gtgagaaact gcatatcccc gacttcaatg
     3001 tgacggcgga gatcgtgacg cttgaattcc acggccatac cgcgatctat tgggacggtg
     3061 tgctccagtc ggtggagata ccggccggat tcatggggtt cg
//
LOCUS       DAOSWW010000227         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23042_length_3101_cov_4.071898, whole genome shotgun sequence.
ACCESSION   DAOSWW010000227 DAOSWW010000000
VERSION     DAOSWW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23042_length_3101_cov_4.071898"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..287
                     /locus_tag="PK442_05665"
     tRNA            210..287
                     /locus_tag="PK442_05665"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Pro,seq:ggg)
     gene            351..1187
                     /gene="rsmA"
                     /locus_tag="PK442_05670"
     CDS             351..1187
                     /gene="rsmA"
                     /locus_tag="PK442_05670"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOO87171.1"
                     /translation="MGREQKSFKNKKSLGQNFLVDRNILRHILEQADLCVDDTVLEIG
                     AGQGVLTRELAASPCSFVFSVEIDEGLSEYLRDITLLYPNTFSLLWGDALAVDYSSLS
                     PVPTKVVANIPYNITTPLLWRLLEQLPEAGTGYFLLMVQKEAAERITAPPGTKERYPL
                     GITLELMGKARSVRTVHPQSFRPVPAVESSLLEIRLSGMHATLPKDSLWRNLLRSSFA
                     QRRKKLSNNLRGFYPSVSWSEAFERTEIHENARAEELTGEQWLALWFFLKTFEKKSLS
                     PE"
     gene            complement(1220..1504)
                     /locus_tag="PK442_05675"
     CDS             complement(1220..1504)
                     /locus_tag="PK442_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOO87172.1"
                     /translation="MYTVTKAELITEVAKATGLNKKESGAAVGAVFEAIEAALEKGEK
                     IQLVGFGTFEVRERAAREGRNPQDPKKVIKIPAKKIPVFRPGKALKDKVE"
     gene            complement(1634..2950)
                     /gene="der"
                     /locus_tag="PK442_05680"
     CDS             complement(1634..2950)
                     /gene="der"
                     /locus_tag="PK442_05680"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOO87173.1"
                     /translation="MPVVSIIGRPNVGKSSLFNRLLGRREAIVDDMPGVTRDRLYGEA
                     EWRDRKFFVVDTGGFLARDENEFVEGMRVQVKLAIEESDLVLFVIDGREGPVWMDEDV
                     ADMLRKSGKPVIVVANKIDDGVHEDDVFQAYTLGFEEVIGISAEHKRYIYDLQDLIVS
                     KLPPEKEQPSETGEIRVAIVGRPNVGKSSILNSLAGEERALVSNIPGTTRDALDTLVT
                     INGTVFNFIDTAGLRRKSRVENDIEYYSFVRTLQAIDRCDVALLVLDAEEPFTDQDKK
                     LAALVVERGKGIVLLLNKWDLLGNNEKLGDSMRKKFRDEMVFLQYAPFAFISALTGRG
                     MHRISEQILHVAENRNRRIGTTQLNRLVRDILAFDTLPTDQKGRPFKIYYCTQSGVEP
                     PTFIFFVNHPDLASPAFENHMEKALRGLEDFSGTPIRIFWRGKEEK"
BASE COUNT          741 a          795 c          741 g          824 t
ORIGIN      
        1 tgtctccgaa tgtcaatgac tgctgctctt ttcatagcgt ctcctccttg aaatgttcgg
       61 agggcacttt ttgaagcggc gatttttgtg taactgccgg ggtggaaaaa agggtgattt
      121 tcagctgatg ttcgcaacat ggaatatttc cactttaatt tggatcattg accgcaagcg
      181 cgtttcaatg tagaatacca aggtcgaaac gggacgtagc gcagcctggt tagcgtacct
      241 gcatggggtg caggtggtcg gaggttcgaa tcctctcgtc ccgaccagaa tattaaagcg
      301 tgggtatacc tccgcttttt ttgtattttt ttgaggagcg aaaagacaga atgggaagag
      361 aacaaaagag ttttaaaaat aaaaaaagcc tcgggcagaa ctttcttgtc gaccggaata
      421 tacttcgcca tattctggaa caggccgatc tttgcgtgga cgataccgtt ctggaaattg
      481 gagccggcca aggagtgctg acaagggaac ttgcggcctc tccgtgctcg ttcgttttct
      541 ctgtagaaat cgatgaaggg ctttcggagt atctgcggga catcactctg ctctatccaa
      601 acacgttttc tcttctgtgg ggagatgcgc tcgccgtgga ctattcttcc ctttctcccg
      661 ttccaacgaa ggttgtagca aacatcccat acaacattac cactcccctg ctctggaggc
      721 tcctggagca actcccggag gccgggacgg gctatttcct ccttatggta cagaaagagg
      781 cggcagagag gattacagcc cctccaggaa caaaggaacg ctaccccctt gggataacgc
      841 tcgagcttat gggaaaggcg cgaagtgttc ggaccgtcca tcctcagtct ttccgtccgg
      901 ttcctgctgt tgaatcatcg cttcttgaaa tccgtctttc cggaatgcat gcaactctcc
      961 cgaaggatag cctctggagg aatctcctac gttccagttt tgctcaaagg cgtaagaagc
     1021 tctcgaacaa tctgcgcggt ttttatcctt cagttagctg gagcgaggct tttgaacgta
     1081 cggagataca tgaaaatgca cgggccgagg aactgacggg agaacagtgg ttggcgttat
     1141 ggtttttttt aaagaccttt gaaaaaaaga gcctctcccc ggagtgagcg ggaagaggca
     1201 aaaaaggaag gaaagaggcc tactctacct tgtccttcaa agctttgccg ggccggaaaa
     1261 caggaatctt cttcgcgggg atcttgataa ctttcttcgg atcctgggga ttacgtcctt
     1321 cacgagccgc tctttcccgg acctcaaacg taccgaaacc aacgagctga atcttctcgc
     1381 ccttttcaag agcggcttcg atcgcctcaa aaaccgctcc taccgccgcg ccggactcct
     1441 ttttgttcaa gccggtcgcc ttcgctactt ctgtgatgag ttctgccttg gtcaccgtat
     1501 acagcctcct ccaaatatga tatgaatctg aaactgactc cgaacctggt tcgttttgat
     1561 aaagccttgc ggtatccgaa cctatcagtt ctttattatt accagcaact gcaattccgg
     1621 tcaagcggaa tcttcatttt tcctcttttc cacgccaaaa aatgcgaatt ggtgtgccag
     1681 aaaagtcctc cagacctctc aaggccttct ccatatggtt ctcaaaggcc ggagaggcta
     1741 ggtccggatg gttaacgaag aagataaatg taggaggttc tacgccggat tgcgtgcagt
     1801 aataaatctt gaacggccgt cctttttggt cggtgggaag cgtatcgaac gcaagaatgt
     1861 cccgtacaag acggttcaat tgagtggtac cgattcttct gtttctgttc tctgcgacat
     1921 gaagaatctg ttcgcttatt ctgtgcatcc ctcttccagt taatgcggaa atgaatgcaa
     1981 acggagcgta ctgtagaaag accatttcat cgcggaattt ttttctcatt gaatccccga
     2041 gtttttcgtt attccccagc agatcccatt tgttgagcag aagtacgatc cctttaccgc
     2101 gttcgacgac cagagctgcg agttttttgt cctgatcggt aaaaggttct tcggcgtcga
     2161 gaacaagtaa agcgacatcg cagcgatcaa tcgcttgcag ggttctgaca aacgagtagt
     2221 attcaatgtc gttctccact ctgctttttc tgcggagtcc ggcagtatcg atgaagttga
     2281 aaacagttcc gttgatggta acgagcgtat ctaaagcgtc gcgagtcgtg ccggggatat
     2341 tgctcaccaa cgcccgctct tctccggcca aactattcag tatgcttgat ttgccaacat
     2401 ttggacgacc gacaatcgca acgcggattt ctccggtttc cgaaggctgc tccttttcgg
     2461 ggggcagttt tgaaacgata agatcctgaa gatcatagat ataccgtttg tgctcggcgc
     2521 taatacctat gacctcttcg aagccgagag tgtaggcctg aaagacgtcg tcctcatgaa
     2581 cgccgtcgtc gatcttgtta gcaacgacga tgactggttt tcctgatttc cgaagcatgt
     2641 ccgccacgtc ctcgtccatc cagacgggtc cttccctacc gtctatgacg aagagtacaa
     2701 ggtcgctctc ttcgattgcc agcttcacct gcacgcgcat tccttctacg aactcattct
     2761 cgtcccgcgc aagaaatcca ccggtatcaa cgacgaaaaa ctttctgtcg cgccattccg
     2821 cctctccgta aagcctgtct cttgttactc cgggcatatc gtccacgatt gcctctcgtc
     2881 ttcctagaag gcgattgaac agcgacgatt tgccaacatt cggtcttcca attattgata
     2941 ctactggcat tgcctcttcc tccccgataa agctcaatca atccagacgg taacttcagg
     3001 aaaaccattt ctggaacagc ctatggaaaa aaacggagcg atgatatctt gaacatccga
     3061 taccgagcac acagtcaccc aaaaatgaag cggagggtcg c
//
LOCUS       DAOSWW010000228         3100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23058_length_3100_cov_3.601314, whole genome shotgun sequence.
ACCESSION   DAOSWW010000228 DAOSWW010000000
VERSION     DAOSWW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23058_length_3100_cov_3.601314"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /gene="glgP"
                     /locus_tag="PK442_05685"
     CDS             <1..892
                     /gene="glgP"
                     /locus_tag="PK442_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOO87174.1"
                     /translation="EVSRNMFTMPEIDWVTNGVHPGTWVSTGMRRLFNTHIPGWENDP
                     GRLVQAVKIPPEDIWSAHQASKLRLLAGIMEQTGQQFEPDILTIGFARRAATYKRADL
                     LFSNMKRLLEVTSGKIQLVFAGKAHPKDDGGKAVLQRLLSASQKVGEMIPIVFLENYD
                     MEKGAMITQGVDLWLNTPLRPREASGTSGMKCTLNGVMNFSVLDGWWIEGWIEDVTGW
                     SIGPDPTESEMVHYDESLDANDLYDKLENKIIPTYYNNREKWIWMMQRTIAMNGSFFN
                     THRVVREYCEKAYNISFRGM"
     gene            899..2365
                     /locus_tag="PK442_05690"
     CDS             899..2365
                     /locus_tag="PK442_05690"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOO87175.1"
                     /translation="MATLHASREIKVLHVSLEAVPFCKVGGLADVAGSLPPSLRKSGT
                     DCRLLLPAWDGVLDTARKRGLKLVRIPRSVDVAIRWKVYNGTLWMSSGEDFPVYFLES
                     PFFEKKQIYPQQLDPESVLPFVFLSLAALELADATEWDADVLHCHDWGTAPLISALLW
                     HRYYRFMFSQYKTVFTIHNLAHQGLLPLHSLRDWGLVDDAFTLNGLEFYGGANLMKGA
                     ITGASAVTTVSPTYSHEIQTENGGVGLGGLLRSQNSKIRGILNGLDTEYWNPATDSLL
                     FQNYSPDDLSGKTRCRNALLDRLGWKEDGRPLFTSVGRMVDQKGFDILLPAIPRMVEW
                     GCRLFLVGSGQREYETAIQEAAAKWPDAVASFVGYDEVIAHQAYAAGDFFLMPSKFEP
                     CGLSQLISLRYGALPVVRSVGGLADSVFEYGSPEGNGFLFSEYSAEGLLSAVGRSLDV
                     FRNDAAGYRSVQKKAMRSDFSWDRSAPLYIDLYRSLLS"
     gene            2438..>3100
                     /locus_tag="PK442_05695"
     CDS             2438..>3100
                     /locus_tag="PK442_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOO87176.1"
                     /translation="MQTGKYGRTMGMVLAGGKGERLMPLTKYRAKPAVHFAAKYRIVD
                     FALSNLVNSGIFAIYVLVQFKSQSLNEHIERGWQFGGALGGRDFLITLVPAQMWRGEH
                     WFQGTADAIYQNLHLITLYDADRICIFAADHIYKMDVGQMLEYHMNKQADVTVAANVV
                     PSSEASAFGCIHVDDSGRIIDFVEKPAHPPEIPGKPGCSYVSMGNYIFEREILEESLI
                     EDA"
BASE COUNT          693 a          862 c          867 g          678 t
ORIGIN      
        1 cgaggtcagc cggaacatgt tcaccatgcc ggagatagac tgggtcacca acggcgtgca
       61 tccgggcaca tgggtgagca ctggtatgcg caggctcttc aacacgcata ttccgggctg
      121 ggagaatgat cccggacggt tagtccaggc agtgaagatc cctcccgagg atatctggag
      181 cgcgcaccag gcgtcgaaac tgcgcctcct cgccggaatc atggaacaaa ccggacagca
      241 gttcgaaccg gatattctga ccataggctt tgcacggaga gccgcgacgt acaaacgtgc
      301 ggatcttctt ttttccaata tgaaacgcct tcttgaagtg acctcaggca aaatacagct
      361 cgtcttcgca gggaaagcgc acccgaagga cgacggaggc aaggctgttc tccagcgtct
      421 tctgagcgct tcccagaagg ttggagagat gatccccatc gtcttccttg agaactacga
      481 catggaaaag ggagcgatga tcacacaggg cgtcgatctt tggctgaata ccccgctccg
      541 cccgcgggaa gcgtccggaa caagcggcat gaagtgcacg ctgaacgggg tcatgaactt
      601 ttccgtcctc gacggatggt ggatagaagg gtggatcgag gacgtcaccg gatggtcgat
      661 aggccccgat cccacggagt cggagatggt ccactacgac gaatcgctgg acgcaaacga
      721 cctctatgac aagctggaga acaaaatcat tccgacctac tacaacaacc gcgaaaaatg
      781 gatctggatg atgcaacgga ccattgccat gaacgggagc tttttcaata cgcatcgcgt
      841 ggtccgcgag tactgcgaaa aggcgtacaa catcagtttc cgggggatgt gatccgcagt
      901 ggcgacactt catgcttcaa gagaaatcaa ggttcttcac gtaagcctcg aagccgtccc
      961 gttctgtaag gtgggagggc tggcggacgt tgccggctcg ctgccccctt ccctccgaaa
     1021 gagcggtacg gactgccgcc tgcttcttcc cgcgtgggac ggcgttcttg atactgcgcg
     1081 caagcgcggc ctaaaactcg tccggattcc tcggagcgtc gatgtcgcca tccgctggaa
     1141 ggtgtacaat ggcacgctat ggatgtcctc cggcgaggat ttccccgtct attttctcga
     1201 gtctccgttc ttcgagaaga aacagatcta tccgcagcag cttgacccgg agtccgtgct
     1261 tccgtttgtc tttctttccc tggcggcgct tgaactcgct gatgccacgg aatgggatgc
     1321 cgacgtgctg cactgtcacg actggggaac agccccgctg atcagcgcgc tgctctggca
     1381 caggtactac aggttcatgt tttcgcagta caagacagtc ttcaccatac ataatcttgc
     1441 gcatcagggc cttctccctc tccactcgct ccgcgactgg ggacttgtcg acgacgcttt
     1501 tacgctcaac ggattggagt tctacggggg cgcaaacctc atgaagggcg cgatcacagg
     1561 cgcttccgct gtaactaccg tcagcccgac ctattctcat gagatccaga ccgagaacgg
     1621 aggggtcggt ctcggggggc ttctcaggag tcaaaactca aagatacgcg gcattctcaa
     1681 cggcctggat actgagtact ggaacccggc cacagacagc ctcctttttc aaaactattc
     1741 tccggatgat ctctcgggga aaacccgatg ccggaatgcc cttcttgacc ggctcggatg
     1801 gaaagaagac ggtcggccgc tctttacatc ggtcggcagg atggtggatc aaaaggggtt
     1861 cgatattctc ttgcccgcga tcccgaggat ggtcgaatgg gggtgcagac tctttcttgt
     1921 cgggagcggg caacgtgaat acgagaccgc aattcaggaa gccgccgcaa aatggcccga
     1981 cgccgtcgca tcatttgtag gatacgacga agtgattgca caccaggcct acgccgccgg
     2041 agactttttt ctcatgccct ccaagtttga accgtgcggt ctctcgcagc ttatttctct
     2101 gcgatatggc gcccttcctg ttgtccgctc cgtaggaggg cttgccgatt cggtcttcga
     2161 gtacggctct cccgagggca acggctttct tttctccgag tactcggcgg aaggtcttct
     2221 ctccgccgtg gggaggtcgc tcgacgtttt taggaacgac gcggcaggat atcgttccgt
     2281 acagaaaaaa gcgatgcggt ccgacttttc ctgggatcgt tcggcgccgc tctacatcga
     2341 tctctatcgt tcacttctgt cctgagcgtg aactacagac gggaaaacgc agtacgacat
     2401 tcgtcaacgc catcttgaga aggagggggg aaacagcatg cagacaggaa aatacggccg
     2461 tacaatggga atggtgcttg ctggagggaa aggggagcgg cttatgccgc tgacgaaata
     2521 ccgggcaaaa cccgcagtcc attttgcagc aaagtaccgt atcgttgatt ttgcgctttc
     2581 caatctggta aacagcggca tttttgctat ttatgtactg gtgcagttca aaagccaatc
     2641 cctgaacgaa cacatcgaaa gaggatggca gttcggcggc gcccttggcg gacgcgactt
     2701 tttaattacg ctcgtcccgg cgcagatgtg gcgcggcgag cattggttcc agggtactgc
     2761 ggacgccatc tatcaaaacc tccatcttat tacgctctac gatgcggaca gaatttgcat
     2821 ctttgccgcc gaccatatct acaagatgga tgtgggccag atgctcgaat atcacatgaa
     2881 caaacaggca gacgttaccg tagctgcgaa tgtcgtaccg tcgtcggagg cttccgcctt
     2941 cggatgcatt cacgtcgacg attcgggacg catcatcgac ttcgtcgaaa aaccggctca
     3001 tcctcccgag atccccggca aacccggatg ttcatacgtc tccatgggga attatatctt
     3061 cgagcgcgaa attcttgaag agtccctcat cgaagacgcg
//
LOCUS       DAOSWW010000229         3099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23066_length_3099_cov_4.071616, whole genome shotgun sequence.
ACCESSION   DAOSWW010000229 DAOSWW010000000
VERSION     DAOSWW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23066_length_3099_cov_4.071616"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1445
                     /locus_tag="PK442_05700"
     CDS             171..1445
                     /locus_tag="PK442_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOO87177.1"
                     /translation="MISSRRWEAPLAERMRPRILDEFSGQEHLLGKDAPLRKNLESSV
                     VPSCILYGPPGVGKTTLVRLMAAITSREILEINAVSAKVAELRDLVKEAENLKTLQSG
                     RSAIAFVDEIYHFNRSQQNALLPSVEKGDIILVGTTTENPWFEINKTLLSRMVVFQLN
                     PLSETDLSELLRRALGDDERGLGQLGIQTDDDALRTLASMAGGDARQALTRLEFICSA
                     VAARGGSALTREFILETLPLAAVRYDKSSDDHYAVISALIKSIRGSDPDAAVYWLARL
                     LSGGEDVRFICRRLLISAAEDIGLADPQALVVASAATQAVEMTGMPEARIILSEAVLY
                     LAAAPKSNSAYLAVSAAMKGVESGDLQSVPFHLRPDGSGYIYPHDDPRHWVPQQYMTE
                     QKRFYFPGDLGSERSIGERLRKFWRRFSTHSE"
     gene            complement(1452..2048)
                     /locus_tag="PK442_05705"
     CDS             complement(1452..2048)
                     /locus_tag="PK442_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOO87178.1"
                     /translation="MKVLVSPLEDPFGFTLPAPQGKSVVLVLSGGVWTNGEDFQMSAE
                     TLQRFVAGVRAADALGCALLFSGGFPGRATEGQIYEMVARAAGDMKYNGPLLVEGTSR
                     TTWENMKFSAPYIKELKAEDVVLVTSAYHIKRSLFFAGRFFKGYRIHPYPSGRLSELG
                     KVTVMDFLPSPASFQYISAAFRELVGMAVYRLLPEQFE"
     gene            2059..2223
                     /locus_tag="PK442_05710"
     CDS             2059..2223
                     /locus_tag="PK442_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87179.1"
                     /translation="MEKKYKEKARMNKKTEAPKRLVPSADFLQRKKKALQKTAAIKNP
                     GGVTKAPRAL"
     gene            complement(2246..2944)
                     /locus_tag="PK442_05715"
     CDS             complement(2246..2944)
                     /locus_tag="PK442_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO87180.1"
                     /translation="MESNSLSPAMNQGLRQIVYEKLKEAIVDGTIKPGSKLSEIELAE
                     RMAVSRTPVREAIRQLAQTGLVTLTPRRGAFVTMPTLKDASDLYELRTELEILAVANL
                     CSLVHSSELIDDLHRFRDVFSAMTNNTSAEKYVNEDKAFHAMIYQAVTNRFLAMMLHN
                     ISDLIHLCRPFSVENSPITVFTWGHLAIIDAILEGDLEKAKEETRKHIVVSKEGLLSF
                     LKNRASKNGEVAEA"
BASE COUNT          772 a          813 c          797 g          717 t
ORIGIN      
        1 ggcaatctca caacagaaca gtggttcaat ctcgcaggtc tctctcccgt atggctggac
       61 cagccggtaa aggaggtttt gtgccatttg ctgcacgccc gatatcctct cgcgcgattg
      121 gtcgatgata ccgacgattt tcttcttctg ccggaaacgg tgatggtaca gtgatctcat
      181 ccagacggtg ggaagcccct ctggctgaaa gaatgcgccc gcggatactc gacgagttct
      241 cggggcagga acacctgctt ggaaaggatg ctccgctgcg aaagaacctg gagagctctg
      301 tcgtgccaag ctgtatcctc tacggacctc ctggcgtagg gaagaccacg cttgttcgtc
      361 tcatggcggc gataacctca cgagagatac tcgaaatcaa tgcggtctcc gcgaaggttg
      421 cggaacttcg cgatcttgta aaggaagcgg aaaaccttaa gacactccag tccggaagaa
      481 gcgccatcgc tttcgtggac gaaatatatc atttcaaccg aagtcagcag aacgcccttc
      541 taccgtccgt cgaaaaaggg gatattatcc ttgtaggtac gacgaccgaa aacccatggt
      601 tcgaaatcaa caaaacgctt ctctcaagga tggtcgtctt tcagttaaat cctctttccg
      661 aaacggatct ttctgagctg ctgcgcaggg cactgggcga tgacgagagg gggttggggc
      721 agctgggcat tcagacggat gatgatgccc tccggacact cgcttcgatg gcaggaggcg
      781 atgcgcgtca ggcgctaacg cgccttgaat ttatatgttc agccgtggcc gcgcgcggag
      841 gctccgcgct caccagggag tttatccttg aaacgctccc cctggcggca gtcaggtacg
      901 ataaatcctc agatgatcat tatgctgtta tctcagcgct cataaaaagt atccgcggct
      961 ctgatcccga tgcggccgtg tactggctgg cgcggctcct ttcaggaggt gaagacgtgc
     1021 ggtttatctg ccgccgcctt ctcatctccg cagccgagga cattggtctt gcagaccctc
     1081 aagcgctcgt cgttgcctcc gcagcaactc aagctgtgga aatgaccgga atgcctgaag
     1141 cgcgcatcat tctttcagaa gcggttctat accttgctgc cgctccgaag agcaacagcg
     1201 cctatcttgc ggtgagtgca gcgatgaagg gcgtggaaag cggagatctt caatctgtcc
     1261 ccttccatct tcgtccggat ggctcaggat acatctatcc gcacgatgac ccgcgccact
     1321 gggtgccgca gcagtacatg acggaacaga aacgttttta ttttcccggg gatcttggaa
     1381 gcgaaagaag tattggagag cggctcagaa agttctggag acgtttcagt acccattccg
     1441 agtaatgttt attattcaaa ctgctccggg agcagtctgt agactgccat tccgaccagt
     1501 tcccgaaacg cggcagaaat gtactgaaaa gaagcggggg aggggagaaa gtccattacg
     1561 gttaccttcc caagctcaga aagccgcccg gaagggtatg ggtgtatacg gtaccccttg
     1621 aaaaaacgcc ctgcgaagaa gagcgaccgt tttatatgat acgcggacgt gaccaaaacg
     1681 acgtcttccg ctttcaattc ctttatataa ggcgccgaaa atttcatatt ttcccacgtc
     1741 gtcctggaag tgccttctac cagtaaagga ccgttatact tcatgtcgcc tgctgcgcgc
     1801 gcaaccattt cgtagatttg cccttccgtg gctctgccgg gaaaacctcc agagaagaga
     1861 agggcgcacc ccaacgcatc tgcagcccgc actccggcga caaaacgttg caatgtttct
     1921 gcgctcatct ggaaatcttc tccgtttgtc catacccctc cggagagtac caataccaca
     1981 cttttcccct gaggggccgg cagagtaaat ccgaaaggat cttcaagcgg ggacacaaga
     2041 accttcacgc tccagggaat ggagaagaag tacaaagaaa aagccagaat gaataaaaaa
     2101 acagaggcgc cgaagcgcct tgtaccgtca gcagacttcc tgcaaagaaa aaagaaagcg
     2161 ctgcagaaaa cagctgcaat aaaaaatccg ggaggggtaa caaaggctcc cagggctttg
     2221 taaatgaagt acagccaggt catgcttaag cctcagctac ctcgccgttt ttcgacgcgc
     2281 gattcttcaa gaaactcaga agaccttcct tgcttactac aatgtgcttg cgggtttctt
     2341 cttttgcttt ttccagatcc ccttcaagga tggcatcaat aattgcaagg tgtccccatg
     2401 taaaaaccgt gatcggcgag ttctcgacag aaaagggtct gcacaggtga ataaggtcgg
     2461 atatgttgtg cagcatcatc gcgaggaatc tatttgttac cgcctgatag atcattgcgt
     2521 gaaacgcttt atcctcgttg acgtacttct ctgcgcttgt gttgttcgtc atggcagaaa
     2581 agacgtcgcg aaaacgatgt aaatcatcga tgagttcact ggaatgtaca agagaacaga
     2641 gattcgcgac agccagaatc tcaagttcgg tccggagttc atacaggtcc gaagcgtcct
     2701 tcaaggtagg catcgtgacg aaagctcccc gacggggagt cagggtcaca agccccgtct
     2761 gggcaagctg acgtattgct tccctgacgg gagtcctcga aacagccatt ctttcggcga
     2821 gttcaatttc cgaaagtttt gatcctggtt tgatcgtgcc gtcaacaatc gcttccttca
     2881 acttttcgta gacaatctgt cgaagacctt gattcatcgc cggcgataat gagttgcttt
     2941 ccacagataa cgacctccca tgcagaacaa tgtacccatt atactgcatg ggaagtgctt
     3001 tcaaaacccc atttcatgtg ttaggttcaa aggcaggaaa tattctctcc tctgcgctcg
     3061 cgttccagat ggtcgtacca gagagaaagc gcatagatg
//
LOCUS       DAOSWW010000230         3094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23121_length_3094_cov_3.680158, whole genome shotgun sequence.
ACCESSION   DAOSWW010000230 DAOSWW010000000
VERSION     DAOSWW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23121_length_3094_cov_3.680158"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK442_05720"
     CDS             <1..220
                     /locus_tag="PK442_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87181.1"
                     /translation="GVAAMFFGGANPFWGWIGCLIFGFADSLGSRLQSLGLPSQFILM
                     IPYVVTVVVLTLAMMRKKRAETARKSAI"
     gene            288..1295
                     /locus_tag="PK442_05725"
     CDS             288..1295
                     /locus_tag="PK442_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOO87182.1"
                     /translation="MATTAKKPVKIIHDCDPGHDDAVAILMAAVHPMIDLKAVTVVAG
                     NQTLEKTVRNALNVCSASKRCADIPIAAGMSRPMVREQVIAGDIHGESGLDGPVFGEP
                     ELGLDTRHAVDLIIDLLLDSDDPITLVPTGPLTNIAMAIRKEPEIVEKIERIVLMGGA
                     YQHGNVTPSAEFNIYADPEAAHVVFTCGRPIVMMGLDVTRKANATAEMVAKVRSLGNP
                     QAILFAEMMEFFAKSQKEVFGWDAPPLHDPTCIAWLIDPTCFTTKPMRVEIELRGSLT
                     YGRTSCDYFGLEHQEGPGKELYEGGGALARPTGPNAEVAVDVDFEKFWKIIYDCFALY
                     K"
     assembly_gap    1344..1443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1538..2833
                     /locus_tag="PK442_05730"
     CDS             1538..2833
                     /locus_tag="PK442_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HOO87183.1"
                     /translation="MDTLNKTLRLHRDEYIARLLELASLDTAVIGHGIEGGGEERGQL
                     WMEALLREMGAETVREPLTEEIIQDGLARFGEGNPGHVYSGDGFSRWNLIGHFKGGEG
                     RSLLFDGHIDTMPSGPRELWSFDPLDAQMRDGKIWGLGTCDMKGGLLASVLGVKLLQD
                     AGIPLPGDVHILSVVDEEGGGNGSLAAMLSGHRADAAVVCEPSNGSVTVAHMGFVFFK
                     VEVRGVALHSGSKWKGVNAIEKAVLLMGALNDLEHEWLMRYKHPLLPPPTLNIGVIEG
                     GTAGSTVPDRCVFKLCLHYHPETMSHDSVVKEVTDRLLLRAAGDTWLRDNPPEISIYQ
                     AGGAFEMEREHSFVRTVEESIASVRKEAPPVVGSPAGNDARLLRNIGTMPVVITGPGR
                     QEQCHSPDEYIEANEYLEYIEIYARLILDWCGRHKEERS"
     gene            2830..>3094
                     /locus_tag="PK442_05735"
     CDS             2830..>3094
                     /locus_tag="PK442_05735"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOO87184.1"
                     /translation="MNKRVLIAGESWVKHISHIKGFDIFTSCEYETGITWLKEALEKH
                     GFEVVHLPGHEVADGFPFTAKELAAYGAVALSDIGANSLLLSGD"
BASE COUNT          558 a          903 c         1019 g          514 t
ORIGIN      
        1 gggcgtggcg gcgatgttct tcggcggcgc caaccccttc tgggggtgga tcggctgtct
       61 gatcttcggt ttcgccgact ccctcggatc gcggttgcaa tcgcttggtt tgccgtcgca
      121 gttcatcctg atgatcccgt acgtcgtcac cgtggtggtg ctcaccctcg ccatgatgcg
      181 caagaagcgc gcggagacgg cgcggaagag cgcgatataa cgtttttttc ggaaacggat
      241 tttcaccgcg cctcccgcgc ggaagtttga gaaaaggcgg tgctgcaatg gctacaactg
      301 cgaagaaacc tgtaaagatc attcacgact gcgaccccgg acacgacgac gccgtcgcca
      361 tcctgatggc ggcggtccac ccgatgatcg atctcaaggc cgtgaccgtg gtggcgggca
      421 accagacgct cgaaaagacc gtcaggaacg cccttaacgt ctgcagcgcg tccaaacgct
      481 gcgcggacat ccccatcgcg gcgggcatgt cccggccgat ggtgcgcgaa caggtgatcg
      541 cgggcgacat ccacggcgag tcggggctcg acggccccgt cttcggcgag ccggagctgg
      601 ggcttgacac gcgccacgct gtggacctga tcatcgacct gctgcttgac tccgacgacc
      661 cgatcacgct ggttcccacg gggccgctga cgaacatcgc aatggccatc cgcaaggagc
      721 ctgagatcgt cgagaagatc gaacgcatcg tcctcatggg tggggcgtac cagcacggca
      781 acgtcacccc atcggccgag ttcaacatct acgccgaccc cgaggcggcg cacgtcgtct
      841 tcacgtgcgg acgcccgatc gtgatgatgg ggctcgacgt cacgcgcaag gcgaacgcga
      901 ccgccgaaat ggtggcgaag gtccgctcgc tcggcaatcc gcaggcgatt ctcttcgccg
      961 agatgatgga gttcttcgcc aagtcgcaga aggaggtctt cggctgggac gcgccccctc
     1021 tgcacgatcc cacctgcatc gcgtggctga tcgacccgac gtgcttcacg acgaagccga
     1081 tgcgcgtgga gatcgagctt cgcggctccc tgacctacgg gcggacgtcg tgcgactact
     1141 tcggccttga acatcaggag gggccgggga aggagctgta cgagggaggc ggcgcgctcg
     1201 cgcggccaac cggccccaac gcggaggtcg ccgtggacgt tgacttcgag aagttctgga
     1261 agatcatcta cgactgcttc gcgctctata agtagacgac cggcgaacat gagcgaagcg
     1321 aataagaagc cgcgtcggtc actnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnntaagtag acgaccggcg aacatgagcg aagcgaataa gaagccgcgt cggtcactta
     1501 gagcgcatgt ttctggcgct cggagacgat caaagtgatg gatactctaa ataagactct
     1561 tcgcctgcat cgggacgaat atatcgcgcg tctgctggag ctggcatcgc tggatactgc
     1621 ggtgatcggc cacggcattg agggcggcgg cgaggagcgg ggccagctct ggatggaggc
     1681 cctgctgcgg gagatgggcg ccgagactgt ccgcgaaccg ctgaccgagg agatcatcca
     1741 ggacggtctc gcccgcttcg gcgaggggaa tcccggccac gtctactcgg gcgacggctt
     1801 ctcccggtgg aacctgatcg ggcatttcaa gggcggggag gggcggtcgc ttctcttcga
     1861 cggacatatc gacaccatgc cgtccggccc gagggagctg tggagcttcg accctctcga
     1921 tgcgcagatg cgcgacggga agatatgggg gctcgggacg tgcgatatga agggggggct
     1981 cctagcctcc gtgctcgggg tgaagctgct gcaggacgcg gggattccgc tccccggcga
     2041 cgtgcacatc ctctccgtgg tggacgaaga gggcggaggg aacgggtcgc tggctgcgat
     2101 gctctccggc cacagggcgg acgcggcggt ggtctgcgag ccgtcgaacg gctcggtcac
     2161 cgtggcgcac atggggttcg tcttcttcaa ggtggaggtg cgcggcgtcg cgctgcactc
     2221 ggggagcaag tggaagggcg tcaacgccat cgagaaggcg gtcctgctga tgggggcgct
     2281 caacgacctc gaacacgagt ggctgatgcg ctacaagcac ccccttctcc cgcccccgac
     2341 gctcaacatc ggcgtcatcg aggggggaac ggcgggctcg accgtgcccg accgctgcgt
     2401 ctttaagctc tgcctccact atcacccgga gacgatgagc cacgactccg tggtcaagga
     2461 ggtgacggat cgcctgctgc tccgtgctgc gggcgacacg tggctgcggg acaacccgcc
     2521 tgagatctcc atctaccagg cggggggcgc gttcgagatg gagcgggagc attcctttgt
     2581 gcgcaccgtc gaagagagca tcgcgtccgt gcgcaaagag gctccgcccg tggtcggctc
     2641 cccggcgggg aacgacgccc ggctgctgcg gaacatcggc acgatgcccg tggtgatcac
     2701 ggggccgggg cggcaggagc agtgccactc tccggacgaa tatatcgaag cgaacgaata
     2761 tctggagtac attgaaatct acgcccgatt gatcctcgac tggtgcgggc gtcacaagga
     2821 ggagcgttca tgaacaaacg cgtgctgatc gcgggagagt cgtgggtgaa gcacatctcc
     2881 cacatcaagg ggttcgacat cttcacgagc tgcgagtatg agacgggcat cacatggctg
     2941 aaggaagcgc tggagaagca cggcttcgag gtcgtccatc tgccggggca cgaggtcgcg
     3001 gacggttttc cattcacggc gaaggagctt gccgcgtacg gcgcggtcgc cctctccgac
     3061 atcggggcga actcgctgct gctctcgggc gaca
//
LOCUS       DAOSWW010000231         3094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23124_length_3094_cov_3.329385, whole genome shotgun sequence.
ACCESSION   DAOSWW010000231 DAOSWW010000000
VERSION     DAOSWW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23124_length_3094_cov_3.329385"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PK442_05740"
     CDS             <1..826
                     /locus_tag="PK442_05740"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOO87185.1"
                     /translation="SQAPYLLPSQRWGQRMGNGQVVDYMVADGLTDIFNNYHMGITAE
                     NIVEQYGFTREEQDQLSLNSQKRAEEAIKSGRFKEEIVPVSIPQRKGDPVVVDTDEHP
                     KFGTTMETLSKLRPAFKKDGTVTAGNASGINDGAAALVVMSAEKARELGVKPLAEIVS
                     YASGAVDPKIMGTGPIPASKKALSKAGLAIEQIDLVEANEAFAAQALSVAKDLNFDMS
                     KVNVNGGAIALGHPIGASGARILVTLLFEMEKRGNSTGLATLCIGGGQGIALIVKR"
     gene            844..1500
                     /locus_tag="PK442_05745"
     CDS             844..1500
                     /locus_tag="PK442_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOO87186.1"
                     /translation="MNKMISLEDAVNKIQDGMTVMVGGFLGCGTPHRIIDKLVEKGTK
                     NLTLICNDTGFPEIGVGKMVVNKQLKKVIVSHIGTNPETGRQMHDEEIEVVLVPQGTL
                     AEQIRAAGAGLGGVLTPTGLGTVVEQGKDLVEVDGVTYLLEKPLKADVALIAGEIADT
                     YGNIVYSGSTRNFNNIMATAAEIVIVEAEQVVEAGTLDPNHVVTPGIFVTHIVDGGAK
                     "
     gene            1500..2159
                     /locus_tag="PK442_05750"
     CDS             1500..2159
                     /locus_tag="PK442_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOO87187.1"
                     /translation="MDKNQVKECIAKRVAKELKDGDVINLGIGLPTLVANYVPEGVNI
                     FFQSENGFIGVGPTPAPGTEDPRIINAGGQPVTILPGGVFFDSATSFGIIRGGHVNAT
                     VLGALQVDEQGNLASWMVPGKMVPGMGGAMDLVTGAKKVIIAMEHTAKGNPKILKQCS
                     YPLTAAHQVDYIVTEMGFMKVTPDGIVLQEINPEFSVEEVQAATEAELIIPDDLKQME
                     V"
     gene            2231..2947
                     /locus_tag="PK442_05755"
     CDS             2231..2947
                     /locus_tag="PK442_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOO87188.1"
                     /translation="MKIQDVKKIGIAGAGTMGSGIAQVFAAKGCKVVVTDLGEADLEK
                     SRRLVKLFNANLVEEGLLTEDATKNIVDNISYSTDKKVFSEADLIIEAIIEKMEIKQA
                     FWGEVELIARDDAFFATNTSGLSINEMCSKLQNKKRFIGMHFWNPPHIIPLVELIRAD
                     GTADETVDDLKELMYAINKEPVVVRKDAPGFIGNRLQFAVFREALHILEEGIAGVDDV
                     DLSIDHVEVVPDRPAPAPAH"
BASE COUNT          766 a          742 c          948 g          638 t
ORIGIN      
        1 gagccaggcc ccgtatcttc ttccctcgca gagatggggg cagcggatgg gcaacggaca
       61 ggtcgtggat tatatggtcg ccgacgggct gaccgatata ttcaataatt atcatatggg
      121 catcaccgcc gagaatatcg tcgaacagta tggattcaca agagaggaac aggaccagct
      181 ctcgctgaac agccagaaac gagccgaaga ggcaataaaa agcggccgtt tcaaagaaga
      241 gatagtcccc gtgagcatcc cccagaggaa gggcgatcct gttgtcgtgg ataccgatga
      301 acaccccaaa ttcggtacga cgatggaaac gctttcgaaa cttcgtcctg cattcaagaa
      361 agacggtacg gtaacggcgg gcaatgcgtc ggggatcaac gacggagcgg cagctctggt
      421 tgtcatgagt gcggaaaaag cccgggagct tggtgtgaag ccccttgcgg aaatcgtcag
      481 ttatgcttcg ggcgctgtgg atcccaagat tatgggaacg ggcccgatac ccgcttcaaa
      541 aaaggcgctg agcaaagcgg ggcttgcgat cgagcagata gatcttgtcg aagccaatga
      601 agcgttcgcg gcgcaggctc tttccgttgc aaaggatttg aatttcgaca tgagcaaggt
      661 caatgttaac ggcggagcga tcgcgttggg acaccctata ggcgcctcgg gagccagaat
      721 tcttgtaacg ctgcttttcg agatggagaa acgaggaaac tcgacaggac tggcgacgct
      781 ctgcatcggc ggcggtcaag gcatcgcctt gatcgtgaag cgatagaaag gggatagtcg
      841 gtcatgaaca agatgatttc tcttgaggat gcggtgaata agatacagga cgggatgacc
      901 gtcatggtcg gagggtttct ggggtgcggc acgcctcatc gtattatcga caagctggtg
      961 gaaaagggga cgaagaatct tacgttgatt tgcaacgata ccggcttccc ggagatcggc
     1021 gtaggaaaga tggttgtaaa taaacaactt aaaaaggtaa ttgtctcaca tataggaacc
     1081 aatcccgaga cgggtcgtca gatgcacgac gaggagatcg aagtcgttct ggttcctcag
     1141 ggtacgttgg cggagcagat acgcgccgca ggggcgggac tgggaggagt gctgactccc
     1201 accggcctgg gaaccgttgt ggagcaggga aaggatcttg tcgaagtgga cggcgtcacc
     1261 taccttcttg agaaaccgtt aaaggcggat gtggcgctta tcgcgggaga aatcgcggat
     1321 acatacggca acatcgtgta ttccggttca acgcgcaact tcaacaatat tatggcgaca
     1381 gcggcggaga tagttatcgt cgaagcggaa caggtggtgg aggcgggaac gctcgatccg
     1441 aaccatgtgg tgactccggg tatctttgtg acccacatcg tcgatggagg tgcgaagtga
     1501 tggataaaaa ccaagtgaag gagtgcatcg cgaagagagt tgcgaaagag ctgaaggacg
     1561 gagacgtgat caaccttggg atcggtttgc ccacgctggt ggcgaattac gttcccgaag
     1621 gcgttaatat tttcttccag tccgagaacg gattcatcgg cgtcggcccc actccggctc
     1681 cgggcactga agatcccagg ataatcaatg cgggaggaca gcctgtgacc atactccccg
     1741 gaggagtctt cttcgactcc gccacgtcgt tcggtattat tcgcggaggt catgtcaatg
     1801 ctactgtact cggagctctc caggtggacg agcaaggcaa tctggccagc tggatggttc
     1861 ccgggaaaat ggtgcctgga atgggggggg cgatggatct cgttaccgga gcgaaaaaag
     1921 tgatcatcgc catggaacat accgccaagg gaaacccgaa gattctcaag cagtgcagtt
     1981 atcctttgac ggcggcgcat caggtggatt atatcgttac cgaaatgggg ttcatgaaag
     2041 tgacgcctga cggtattgtt ctgcaggaaa ttaatccgga gttttctgtc gaggaagttc
     2101 aggcggccac ggaggccgag ctgatcatcc cggacgatct gaagcagatg gaagtttaaa
     2161 cgaccggaca gggggctgtt ttgcacaaag agccccctta ttttacttga ttcgggagga
     2221 gcggcgataa atgaagattc aggacgtaaa gaaaatcggt atcgctggag ccggtacgat
     2281 ggggtccggt atagcccagg ttttcgcggc gaaaggctgc aaagttgttg tcacggacct
     2341 tggagaagcc gacctggaaa aatcaagacg tctcgtgaaa ctgttcaatg ccaaccttgt
     2401 cgaagagggg ctcttaacgg aggatgcaac aaaaaatatt gtcgataaca tctcgtactc
     2461 gaccgacaaa aaggtgtttt ccgaagccga tttgatcata gaagcgatta tcgaaaaaat
     2521 ggagatcaag caggccttct ggggcgaggt cgagctgatt gcccgggatg atgcgttctt
     2581 tgcgacgaat acctctggtc tgagcataaa cgagatgtgc tcgaagctac aaaacaaaaa
     2641 acgttttatt ggaatgcatt tttggaatcc ccctcacatt attcccttgg tcgagttgat
     2701 tcgcgctgat ggaaccgctg acgagaccgt cgacgacctc aaggaactta tgtatgcgat
     2761 caataaggag ccggttgtcg ttcggaaaga cgcccccggt ttcattggga atcgccttca
     2821 gttcgccgta ttcagagagg cgcttcacat tttggaagag gggatcgccg gagtcgacga
     2881 tgtcgacctc agcatcgacc acgtggaggt cgtgcccgac cgccccgcgc ccgcccccgc
     2941 gcactgaccc gttccgtcag ccccggccca gcacccgctc cacgctgatc tcgatcgcga
     3001 cgcgctgcgg gttgtcgcgc ggttcgcggt accgcgcgcg gtagcggccg acggcctcgg
     3061 cgaccgcgtc gggatcgtcg accacgcgcg cggg
//
LOCUS       DAOSWW010000232         3057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23560_length_3057_cov_3.551632, whole genome shotgun sequence.
ACCESSION   DAOSWW010000232 DAOSWW010000000
VERSION     DAOSWW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23560_length_3057_cov_3.551632"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..509
                     /locus_tag="PK442_05760"
     CDS             321..509
                     /locus_tag="PK442_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87189.1"
                     /translation="MIAVKDEVRTLLERLPDDCSFEDIQYHLYVLEKVRRSAARVEQD
                     GALEHDEVEKRLHRWLSV"
     gene            494..802
                     /locus_tag="PK442_05765"
     CDS             494..802
                     /locus_tag="PK442_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO87190.1"
                     /translation="MALRLKWSPDAVEDLETIAEYIARDSSSYASSVVSKIVTVAKTI
                     PDFPRSGRIVPEIGDHAIREHVVFSYRLIYRVEPDHITLLAVNHGKRLLEKNPAWCDE
                     "
     gene            complement(863..1705)
                     /locus_tag="PK442_05770"
     CDS             complement(863..1705)
                     /locus_tag="PK442_05770"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOO87191.1"
                     /translation="MNEIRSIRRIVTGKPATDGAGVKLVRVIGYNDTQDFDPFLMLDA
                     FDSTNPEDYVKGFPWHPHRGIETITYLIRGDIAHGDSLGNKGRILDGECQWMTAGSGI
                     LHQEMPKASERMLGTQLWLNLPAKDKMTEPKYNDIVGENVPTVAEEGLNIRIISGNYK
                     GTDGAFRGEYADALYLDAEMAPGAEWLLETDPEATLFIYIVQGAGFFGPSGEGVAEKH
                     AVLFDKGNAFRAKASEAGARIFLMAAKPLGEPIAWGGPIVMNTREELDLAFRELQEGT
                     FIKR"
     gene            1935..2465
                     /locus_tag="PK442_05775"
     CDS             1935..2465
                     /locus_tag="PK442_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO87192.1"
                     /translation="MRYIALLGSPRKDGNTAALLASFLEGAASKGHSGELIRLHEKKI
                     LPCAGCNACKTEGKCILSDDMTALYPLVEEAEVIVHAFPVYWWSMSAQTKLFQDRLYA
                     MDYTHFKGKQLFLLTTYGGEDPNSGPRLCEQSFRDICEFLDMQFRDFIGVCSGTTPVR
                     DNKRALERAFLAGASI"
     gene            2531..>3057
                     /locus_tag="PK442_05780"
     CDS             2531..>3057
                     /locus_tag="PK442_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOO87193.1"
                     /translation="MRSLRIRVILLSVCAFLLTSGIAAGAPSREELLERPAAGVVAGS
                     NVNVRDRPSTRGKSLGRLGEDGYWEELVVVDAAPGDDGQIWYKILSEKLGEGWMHGRY
                     LRFEGLDDPLGRFFVLLRRDFGTNPAMTQQRLGKPLKTERKPFRPEDYPEDVTSVELR
                     YPGGSVVFWIMRKVP"
BASE COUNT          613 a          905 c          890 g          649 t
ORIGIN      
        1 cctcgtcgaa gcgggccgtg tggcacgacg aatagagcga ttccgcgcgt gcggtgatac
       61 cggttttttc aaaaagcatg cggctccgac caaggcgggg ccgcatgctt tttgagacag
      121 gcgtttccca agctgattct tcgcagtttt tcgagcgatg aaagaagccg aaggaacgaa
      181 aagccctgcg atcatcgtga tacaataaat caagaaaatt cgccggatcg gaacttcgcg
      241 gcgatctccc gcgtccggtc gtgttatgag aagttccccc ttgagcggga gcaagggacc
      301 gaaaaggagc gtgaacctcg atgattgcag taaaagatga agtgcgcact cttctggagc
      361 ggcttcccga cgattgttcc tttgaagata tacagtacca tctctatgtt cttgaaaaag
      421 ttagaagaag cgctgcgaga gtcgaacagg acggtgcatt ggagcatgac gaggttgaga
      481 agagattgca tcgatggctc tccgtctgaa atggtctcct gacgcggtgg aagacctcga
      541 gaccatagcg gagtatatcg cgagagactc gtcttcgtac gcctcttctg tagtatccaa
      601 aattgtaact gtcgcaaaaa ctatccccga ttttccgcgg agcgggcgaa ttgtcccgga
      661 aataggcgat cacgcgatac gcgagcatgt agttttcagt tatcgcctga tctatcgcgt
      721 cgaacccgat catatcacgc tgcttgccgt gaaccatgga aagcgattgc tggaaaaaaa
      781 ccccgcatgg tgcgacgagt agccccgtta caccagtgtg catatggtcg aatggcaaaa
      841 aagcccggag tttctggaac ggctatctct tgatgaacgt cccctcctga agctcgcgga
      901 acgccaggtc cagctcttcg cgagtgttca tcacgatcgg accgccccac gctatcggct
      961 ccccaagcgg tttcgcggcc atcagaaaga tgcgcgcgcc cgcttctgag gcctttgcgc
     1021 ggaacgcgtt ccccttatcg aagagcacgg cgtgtttctc cgcgacgccc tctcctgaag
     1081 ggccgaaaaa ccctgcgccc tgaacgatat agatgaagag cgtcgcttcc ggatcggtct
     1141 ccagcagcca ctccgccccc ggcgccatct ccgcgtcgag gtagagggcg tccgcgtact
     1201 ctccccggaa ggcgccgtcc gtccccttgt agtttccgga gatgatgcgg atgttcagcc
     1261 cctcctccgc caccgtcggg acgttctccc cgacgatgtc gttgtacttc ggttccgtca
     1321 tcttgtcctt cgcgggcaga ttgagccaga gctgcgtccc cagcatccgc tcgctcgcct
     1381 tcggcatctc ctggtggagg atacccgatc cggccgtcat ccactggcac tccccgtcga
     1441 gaatgcgccc tttgttgccg aggctgtcgc cgtgcgcgat atcgccccgg atcagatagg
     1501 tgatcgtctc gatcccccgg tgcggatgcc aggggaagcc tttgacgtag tcctccgggt
     1561 tcgtcgagtc gaaggcgtcc agcatcagga acgggtcgaa atcctgcgtg tcgttgtacc
     1621 cgatgacacg aaccagcttc acccccgcgc cgtccgtcgc gggttttcct gtgacgattc
     1681 tgcggatgct tcgtatttcg ttcataactc gttaccctcc ttcggctgca ccgtatgctc
     1741 attgcgatta tagcatttca ttggaagcac accgggaagc cccttgcggc tcgccgacgg
     1801 agttgtgtgg aaatttacca aaacatcgtg tatccttacg tgaaggcaac gatccgccga
     1861 ggtgcgtccg gtcccttcgt ggtctttgtc cagttgcaat actatttcct accctatctg
     1921 aaggagatga tgccatgcgc tacatcgcac tactcgggag ccccaggaaa gacgggaaca
     1981 ccgccgctct gctcgcctcg tttctcgaag gcgcggcttc caaaggacat tcgggggagc
     2041 tcatccggct ccacgagaag aagatactcc cgtgcgcggg gtgcaacgcc tgtaaaacgg
     2101 agggaaagtg cattctctcc gacgacatga cagccctcta ccccctcgtc gaggaggccg
     2161 aggtcatcgt ccacgcattc ccggtgtact ggtggtcgat gtccgcgcag accaagctct
     2221 ttcaggacag gctctacgcg atggactaca cgcatttcaa ggggaaacag ctcttcctgc
     2281 tgacgacgta cggcggcgag gaccccaact ccggtccgcg cctctgcgaa cagtccttcc
     2341 gcgacatctg cgaattcctg gacatgcagt tccgcgactt catcggcgtc tgctccggga
     2401 cgacgcccgt ccgagacaac aaacgagccc tcgaaagggc gtttctcgcg ggcgcgtcga
     2461 tctagcgttc cgtcgacgga catgtcggtc gcgttcgctt caggaaggtg atagaaagga
     2521 ggaaggaacc atgcgctctc tgcgaatacg agtcattctg ttgtctgtgt gcgcgtttct
     2581 tctgacatcg ggcattgccg ccggggcccc ctcgcgtgag gagctgctcg aacggcccgc
     2641 ggccggagta gtcgcaggca gcaacgtcaa cgtccgggac cgcccttcga ccaggggaaa
     2701 gagcctcggg aggctggggg aggacggcta ctgggaggag cttgtcgtcg tggacgccgc
     2761 tccgggtgat gatggtcaga tatggtacaa gatcctctcc gagaagctcg gcgaggggtg
     2821 gatgcacggc cgctatctcc gtttcgaggg actcgacgat ccgcttgggc gcttctttgt
     2881 tctgctccgt agggatttcg ggacgaaccc ggctatgacg cagcaacggc tggggaagcc
     2941 cttgaagaca gagcggaagc ctttccgccc ggaggactac cccgaggacg tgacgtcggt
     3001 cgaactgcgc taccccggcg ggagcgtcgt cttctggatc atgcggaaag taccctt
//
LOCUS       DAOSWW010000233         3054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23595_length_3054_cov_3.898633, whole genome shotgun sequence.
ACCESSION   DAOSWW010000233 DAOSWW010000000
VERSION     DAOSWW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23595_length_3054_cov_3.898633"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..468)
                     /gene="mce"
                     /locus_tag="PK442_05785"
     CDS             complement(67..468)
                     /gene="mce"
                     /locus_tag="PK442_05785"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOO87194.1"
                     /translation="MKPTVVDHIGIAVKSIDEALAFWQSSLGIQCTGVEEVAEQKVKT
                     AFLPIGDTEVELLEATSDESPVAKFIEKKGEGIHHLAIRVDNLEKALEEMKAKGIKLI
                     DESPRYGAGGAKIAFVHPKSTGGILLELSER"
     gene            702..1091
                     /locus_tag="PK442_05790"
     CDS             702..1091
                     /locus_tag="PK442_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87195.1"
                     /translation="MSLITCKLCGRLYDATRGKICSQCLDELDELYPRVREFIRDHSK
                     TSFSVDSLAEDMDVDILRMQSLVDLGYLDRDVPGISGADEEKKKKEELLQQLKGTLNT
                     SAPRERHSDEKASKMYGQERYGTGKKK"
     gene            complement(1238..1696)
                     /locus_tag="PK442_05795"
     CDS             complement(1238..1696)
                     /locus_tag="PK442_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO87196.1"
                     /translation="MFKKILVAVDMSKMSEEVFAYGCSLALRLGAEATFLHVLPHPTL
                     WRGYEPWLPPEIDVEVAEIARKKLDYYFRKAKENNPELENTNYTMVVREGNPSDVIIN
                     YAKEHDMHLVVIGYRGQSTLERLVVGSTASNVGRYAHCSVLIYRPGEQAI"
     gene            1937..2707
                     /locus_tag="PK442_05800"
     CDS             1937..2707
                     /locus_tag="PK442_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOO87197.1"
                     /translation="METALLIVFFSFLGASFGSFLNVVALRSISGKSWSGGERSVCVS
                     CGTVLEWRDLVPLVSWCMLRGKCRRCGTRIGIRYLAVEVIGALAGGLLAWRWGVSYTL
                     LLALAATFGLFLNALTDVEDGYVFDVFPLTMGIIGVALRLPGGWDALFDGLLGAGAGF
                     AVIACIILISRGGMGWGDATLAAGTGSIMGWKMLLLTLYSGFMAGGILALLLLLAGKV
                     RRKDAIPLVPFLALGGVFTLLAGPRILMFFGAAPGWPW"
     gene            complement(2670..>3054)
                     /locus_tag="PK442_05805"
     CDS             complement(2670..>3054)
                     /locus_tag="PK442_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87198.1"
                     /translation="GLEPITLPGVDALVISGSGDRIRAEASAGLALPILNSLGSSEVI
                     HTAASKVSLSFALFFYGGCDGTFDEESSRRILRSLRLDEEKKEFHILPGVDHAFRTVN
                     GTPSFEPLEQMLGITMATLERRRRT"
BASE COUNT          675 a          802 c          789 g          788 t
ORIGIN      
        1 tctcacgctt cagcaacagc tcctcgcaga gctcttctat cgttcgctgc gacataaaaa
       61 aacctcctac ctttcgctga gttccaaaag aataccgccc gtgctcttcg ggtggacgaa
      121 agctattttc gcccctcccg cgccataacg gggcgactcg tctatcagct tgattccctt
      181 cgctttcatc tcctccaggg ccttttcaag attatccacc cggatagcaa gatggtggat
      241 accctcgcct ttcttttcga taaacttggc gacagggctc tcatcggagg tagcctccag
      301 aagctctacc tccgtatcac cgatggggag aaatgccgtt ttcactttct gttcggcgac
      361 ttcttccaca cctgtacact gaattccaag gctcgactgc cagaatgcaa gcgcctcgtc
      421 gattgatttg accgcgattc cgatatgatc cactacagta ggtttcatga gtatttttct
      481 cctttcgata ctttttaccg gatgaaacag aagcatacag gatatcttaa cagaaaagat
      541 ggggagaaac tctctcagaa gaagagaata tttgtataca tgtagtcaga cgacgccaat
      601 tacaccactc cgttctttgg cttttttcct cagccctctt tttcctgccg ctaaagagtg
      661 atatagtatt cggcaagaac acgcagaagg gaggcgtcat cgtgagcctg attacatgca
      721 aactttgcgg gaggctttac gacgcaacac gcggaaagat ctgttctcag tgccttgatg
      781 aactcgacga attatacccc agggtgagag agttcatccg ggatcattca aaaaccagtt
      841 ttagcgtcga tagtctcgcc gaagatatgg atgtggacat cctgagaatg cagtcgcttg
      901 tggatctcgg ctacctcgac cgcgacgttc ccgggatatc cggcgcagac gaagaaaaga
      961 aaaaaaaaga ggagcttttg cagcagctga agggcacgct caatacctcc gctccgagag
     1021 agagacactc agacgaaaaa gcatcgaaga tgtacggaca ggagcgctac ggcacaggga
     1081 aaaagaagta aaaacagcgc cggaaagaac aggtttcccc agttcttttt tccaccgctt
     1141 caacgcattc catggagaga tatgacgaac ccgggacaac cgttttccgc tgtcccgggt
     1201 cgagtttttc ttggcttcac gtatcacagt tcgtttctta gatggcctgc tcccccgggc
     1261 gatagatgag caccgaacag tgcgcgtatc gtccgacgtt cgaggcagtg ctcccgacca
     1321 caagacgctc aagagtgctc tgtcccctgt atcctatgac aaccaggtgc atgtcgtgtt
     1381 cttttgcata gttgatgatc acgtcggagg ggttcccctc tctgacaacc atcgtatagt
     1441 tcgtgttctc cagttccgga ttgttctcct tggccttccg gaagtagtag tccagctttt
     1501 tacgggcaat ttccgctacc tctacatcga tctcgggggg cagccagggt tcgtaccctc
     1561 tccagagcgt gggatgagga agcacgtgaa gaaaggtcgc ctcggctcca aggcgtaagg
     1621 cgagagaaca gccgtaggcg aagacttctt ctgacatttt gctcatatca accgcaacca
     1681 gtatcttctt gaacatggct cttcctcctt tcacatttca ccgaatgaat cggtcgccct
     1741 ttgagaatat tatacaacaa aatactgaaa ccggaatata gaacgcatct ttcttttcat
     1801 tctcttgctc gatctgcggg ggaagctctt ctgcgaaaga aagtttggct gaatgcgctc
     1861 cttgtgctat atatagagat aaggagtgcc tctgcatcaa actggcagcc aattccttcg
     1921 tgaaggattg atgaatatgg aaaccgcgct tctcatcgtt tttttttctt ttttgggcgc
     1981 atctttcggt tcgtttctga acgtcgtcgc gttacggagc atctctggaa aatcctggag
     2041 cggaggagaa cgatccgtgt gcgtctcctg cggaacagtt ctggagtgga gagatcttgt
     2101 gccgctggtt tcatggtgta tgcttcgtgg aaaatgccgt cgctgcggga cgaggatcgg
     2161 cattcgttac ttggcggtag aggttatagg cgctcttgcc ggagggctgc ttgcctggag
     2221 atggggcgtt tcttatacgc tcctcctcgc tttggccgct actttcggtc tttttctgaa
     2281 cgcgctgacc gacgtcgaag acgggtacgt tttcgacgta tttcctctta ccatgggaat
     2341 catcggcgtc gccctgcgtt tgccgggagg ctgggatgcg ctgtttgacg gtctcctcgg
     2401 ggcgggagca ggttttgcag taatcgcctg tatcattctc atttccagag gaggcatggg
     2461 gtggggagat gctacgcttg ctgccggtac agggagcata atggggtgga aaatgctcct
     2521 cctcaccctc tactcaggct tcatggcagg cggaatcctc gcgcttctcc ttctgcttgc
     2581 gggaaaagta cgccgcaagg atgcgattcc gcttgttccg ttcctcgcgc tgggcggtgt
     2641 ttttactctc cttgccggcc cccggattct catgttcttc ggcgccgctc cagggtggcc
     2701 atggtgattc caagcatctg ttccagcggt tcaaaagagg gcgttccgtt cactgtacgg
     2761 aatgcatggt caactccggg cagtatatga aattcttttt tctcctcgtc aagccggaga
     2821 ctgcgtaaaa ttcttctcga cgactcttcg tcgaaagttc catcgcatcc accgtaaaag
     2881 aaaagagcga aggaaagcga tactttcgat gcagccgtgt gaataacttc gctggagcca
     2941 agggagttca gtatcggaag cgcgagcccc gccgaggcct cggcacggat ccggtcgccg
     3001 cttccggaga tgacgagggc gtcgacgccg ggaagcgtta ttggctccag ccca
//
LOCUS       DAOSWW010000234         3053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23613_length_3053_cov_3.056037, whole genome shotgun sequence.
ACCESSION   DAOSWW010000234 DAOSWW010000000
VERSION     DAOSWW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23613_length_3053_cov_3.056037"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PK442_05810"
     CDS             <1..942
                     /locus_tag="PK442_05810"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO87199.1"
                     /translation="LSHMRSESDDVVASVRETIAVAENLGIPGQVLHHKACGRRNRGR
                     VRETMRLLEEARERGVDMTADQYPYAAGSTTLRSILPPWIHEGGVEKLIERLSGLALR
                     ERLKREIPEAEGWENMLRNSGGPEGVMMLYCPKTPDFEGKRLTEIGAMTGKDPLDAAF
                     DIIIENRGSDTACFFQLAEEDVQFVMKHPLVMVASDSIPPAPGAKCHPRTNGTFPRVL
                     GKYVREEKTLRLEEAVWKMSGFPAARLGLSKKGLIREGMDADLVLFDPETVVDGATFE
                     DPFGEPKGISHVFVAGRLAVKNGEYTGAAAGKVLRKV"
     gene            complement(1017..1547)
                     /locus_tag="PK442_05815"
     CDS             complement(1017..1547)
                     /locus_tag="PK442_05815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO87200.1"
                     /translation="MRYTTALPGLIIRGAVEQDVPLILSFIRELAVYEKMLDDVVATE
                     DGLKEHLFGERRVAEVLIAEWEGAPVAFALFFHNFSTFLGRPGIYLEDLYVKPEMRGK
                     GIGKAMLSHIANTAKKRGCGRFEWSVLDWNEPAITFYRSIGAVAMDEWEHFRLSGEAL
                     ETVAEMCAPENVYPKQ"
     gene            1828..3006
                     /locus_tag="PK442_05820"
     CDS             1828..3006
                     /locus_tag="PK442_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO87201.1"
                     /translation="MISVTKLLLGREYSGDRLRYRRGTNSGGAADGLGPVVVWNCTRA
                     CNLLCRHCYASATPYACDAELSTGEAKDFLDTLAAFNVPALLLSGGEPLLRRDLFELL
                     EYARTLGLRTALSTNGTLIDERTAARIMELGVSYAGISLDGSPEVNDAFRGVDGAYNR
                     TITGFRNCRKAGQKAGLRLSMTRSTLKEMPEIFRLVEDEGIARVCLYHLVPSGRGAEL
                     SNEEPSPKEKRAALDFLAEKTLDFAARGVDTEILTVDNHCDGIYLYIREKERGGSRAA
                     EMLDLLSASGGNRSGVAIGAVDWEGDVHIDQFTQEVILGNVREKDFPEIWRGKSDSFL
                     HRLRDRKRHLRGRCRTCGWLEQCNENLRARALAAGDFWGPDPSCYLTDDEIAHGGTSC
                     "
BASE COUNT          601 a          886 c          969 g          597 t
ORIGIN      
        1 ctctcccaca tgcgcagcga gtcggacgat gttgtcgctt ctgttcgtga aaccatcgcc
       61 gtggcggaaa acctcgggat tccggggcag gtgctccacc acaaggcctg cggcaggagg
      121 aaccggggca gggtccgtga gaccatgaga ctgcttgagg aggccaggga gcgcggcgtc
      181 gacatgacgg cggatcagta tccgtatgcg gcggggagca ccacgctccg gagcatcctt
      241 cccccctgga ttcacgaggg aggggtggag aagctgatcg aacgtctctc cggtctcgcg
      301 cttcgcgagc gtctgaaacg ggagatcccg gaggccgaag ggtgggagaa catgctgcgc
      361 aacagcggcg gtccggaggg tgtgatgatg ctctactgcc cgaaaacgcc ggacttcgag
      421 ggaaaacgcc tgacggagat cggtgcgatg acggggaaag atccgctcga cgcggcattc
      481 gatatcatta ttgaaaacag gggttccgac acggcatgct tctttcagct tgcggaggaa
      541 gacgtgcagt tcgtcatgaa gcaccctctc gtcatggtgg cttccgacag cattcctccg
      601 gcgcccggag cgaagtgcca cccacgtacg aacgggacgt ttccccgcgt tctcggcaaa
      661 tacgtccggg aggaaaagac ccttcgtctc gaagaggccg tttggaagat gagcggcttc
      721 ccggcagcac ggctcgggct gtcaaaaaaa ggactcatca gagaaggaat ggacgccgat
      781 ctcgtcctct tcgacccgga gaccgtcgtc gacggagcga ccttcgagga ccccttcggc
      841 gagccgaagg gcatctccca cgtctttgtg gcggggcgtc tcgccgtgaa gaacggcgag
      901 tacaccggcg ccgcggcggg gaaggtgctt cggaaggtgt aagcgtagga tgcaacaaag
      961 agcggggttc cctcggatgg gaccccgctc tttgtcgtat cgagtgagcg ttttcttcac
     1021 tgtttgggat acacgttctc cggtgcacac atttcggcga ccgtttccag agcctccccg
     1081 ctcaggcgga agtgctccca ctcgtccatc gccactgctc cgatggagcg gtagaatgtg
     1141 atcgccggtt cgttccagtc gagcaccgac cactcgaaac ggccgcaccc gcgtttcttt
     1201 gcggtgttcg cgatgtggga gagcatcgcc ttcccgatcc ccttcccgcg catctccggc
     1261 ttcacataga ggtcctccag gtagattccc ggacgtccaa ggaaggttga gaagttgtgg
     1321 aagaagagcg cgaaggcgac gggagcgccc tcccactcgg ctatgagaac ttctgcgacg
     1381 cggcgttcgc cgaacaggtg ctctttcagt ccgtcctctg tggcgacgac gtcgtcgagc
     1441 attttttcat aaacggcaag ttcccggatg aacgagagga tgagcggaac atcctgttcc
     1501 acggcacccc ggatgatcaa gccgggcaga gcggttgtgt agcgcattcg gtaccacctt
     1561 tcacggcgat gaacggcttt aaggccgagg ttcgccatcg gaatttgcgg caattatatc
     1621 aaaagagcgt gtgatttttg cccctgtttc agtggtatga acggcgcttc gcgctttaca
     1681 tccgggaaag ggacaaatac aatgcttgtg gcgtcgtgat ggaacatgtt tgtcccgatg
     1741 atcgaatgac tgctcatcgg gtaatttatc cgtacagcgc cgttggctga tacagctcgc
     1801 cgaatacttt cctcaaggag ggtttcgatg ataagtgtga caaaattgct gctcggacgc
     1861 gagtattccg gcgatcgact gcggtaccgc cgcggaacga acagcggggg cgctgccgac
     1921 ggtttgggac cagttgtcgt ctggaactgc acccgcgcgt gtaatcttct ctgtcgccac
     1981 tgctacgcct cggcgacgcc gtatgcctgc gacgccgagc tttcgacggg cgaggcgaag
     2041 gattttctgg ataccctcgc cgccttcaac gtccccgcct tgctgctctc gggcggagag
     2101 cccctgttgc ggagggacct cttcgagctt ttggagtacg cgcgcacgct ggggttgcgc
     2161 accgcgctct cgaccaacgg gacgctcatc gacgaacgga ctgcggcgcg cataatggaa
     2221 ctcggcgtga gctatgccgg tatcagtctc gacggatcgc ctgaggtcaa cgacgccttc
     2281 cgcggcgtcg acggagccta caacaggacg atcaccggat tcaggaattg ccggaaggcg
     2341 gggcagaagg cgggattgcg cctctccatg acccggagta cgttgaagga aatgcccgaa
     2401 atctttcgcc tcgtcgaaga cgaaggaata gcacgcgtct gcctgtatca cctcgtccct
     2461 tcggggcgcg gtgcggaact ctccaacgag gagccttcgc cgaaggagaa gcgagccgct
     2521 ctggattttc tcgccgaaaa gacgctcgac ttcgccgctc ggggcgtgga taccgaaatc
     2581 cttaccgtgg acaaccactg cgacggcatc tatctctaca ttcgggagaa agaacgggga
     2641 gggagccgcg ccgcggagat gctggatctg ctctccgcga gcggaggcaa tcgctccggc
     2701 gttgcgatcg gcgcggttga ctgggagggg gacgtccata tagaccagtt cacgcaggag
     2761 gtcatcctcg gcaacgtccg tgagaaggac ttccccgaga tctggagggg aaagtccgat
     2821 tcatttctgc atcgtctgcg cgaccggaaa cggcatctcc gcggtcgatg ccgtacctgt
     2881 ggatggctgg agcagtgcaa tgagaatctc cgggccaggg cgctcgccgc cggggatttc
     2941 tggggccccg atccttcctg ttacctgacc gacgacgaga tcgcgcacgg aggcacgtcg
     3001 tgctgatctc ctggaacacg acgcgccgct gcaacctctt ctgcaggcac tgc
//
LOCUS       DAOSWW010000235         3050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23640_length_3050_cov_3.575960, whole genome shotgun sequence.
ACCESSION   DAOSWW010000235 DAOSWW010000000
VERSION     DAOSWW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23640_length_3050_cov_3.575960"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK442_05825"
     CDS             complement(<1..370)
                     /locus_tag="PK442_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87202.1"
                     /translation="MAGTVESLENPKDTAEHRGTSEILLKGTGYGVRMILPETKTDED
                     LLQEALAFSGEAARLTRGIGVVLDFQGRTISRAFLLRFLSEFVWTGAFRVLSWMSYNA
                     DTLELFRASGFSTGEPTAARS"
     gene            complement(473..2218)
                     /gene="mrdA"
                     /locus_tag="PK442_05830"
     CDS             complement(473..2218)
                     /gene="mrdA"
                     /locus_tag="PK442_05830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOO87203.1"
                     /translation="MSDHRPEMNLRLLLWKLFLMFSLLLLAGGLYFFQIVNADAYVRL
                     AANNRLRFIRFAPIRGEIFDRNGVPLAANVTTFDIMGYPLDIEKNNMVENLALLLSRH
                     GIPVSAEDLRRDVKRQFWAPYRVVRVVSNITLAQMADLVADPEFPRQLFPIPVLRRTY
                     PAGSIASNVTGFVGEINENELRQMEGKSYVGGDQIGKTGIERQYEEILRGEPGGESVE
                     VDARGRRVRQIDTYPAGRGEDLHLTLDLGAQRLAADLLQGYRGALIVMDVHDGDVLVL
                     ASSPSYDNNPLSWGVSAKEWNALLSDPQKPMLDRSIAGLYPPASTFKILVGLAALVEK
                     EVTPSTTFHCGGAFTLGNRTFRCWRRSGHGTVNLTGALQNSCDVYFYQVGLKLGITRL
                     LKWVELFGLGRPTGIDLPGEAGGVVAGPDWKKARFKEGWYRGDTVNYSIGQGFLLTTP
                     LQLATMYAVIANGGKLVRPRLANSLPVQAAELNLSPSHMNVIRKGLDYVVRRGTARRA
                     GGFGITVAGKTGTAQNAHGDDHALFVGYAPMDNPKYVAVAMLEAGLHGGSVASPMVGE
                     MLSYLLVPESRMKTP"
     gene            complement(2211..2669)
                     /locus_tag="PK442_05835"
     CDS             complement(2211..2669)
                     /locus_tag="PK442_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87204.1"
                     /translation="MIYLLAWYAQDFLWVLGAGKVLVPEIFLLTLVFLSLFRSEDGVR
                     VVWAAFLGGVLWDLRWTGFPGISSLIYVLSLMSVRWIWHSIPASGRTITLFGFLLWSA
                     NMPIALGRIFLWGARGIEVWRAYLLQQSHIVPLVLLACAAYAWRLKNQDV"
     gene            complement(2666..>3050)
                     /locus_tag="PK442_05840"
     CDS             complement(2666..>3050)
                     /locus_tag="PK442_05840"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOO87205.1"
                     /translation="GRVSSVEDDFAWVELLSSSSLMIPVVIEETRDLGVVAGDGEGGV
                     WLLYVPENRMLKSGMRVSTAMVSEALPPGIPLGSLGEERRETSGGYTEWRILPGASLS
                     QLYALEVLGLRTPSQEKPAGGRSGL"
BASE COUNT          668 a          992 c          833 g          557 t
ORIGIN      
        1 cactcctcgc ggcagtcggt tcgccggtgg aaaaaccgga tgcgcgaaag agttcaagcg
       61 tatccgcatt gtaggacatc cacgaaagaa cgcggaaagc ccccgtccag acaaattccg
      121 aaaggaaacg gagcaggaaa gcccgggaga tcgttctccc ctgaaagtcg agaacaacgc
      181 cgatgccgcg ggtcagacgg gcagcctctc ccgagaaggc aagcgcctct tgcagaagat
      241 cttcgtccgt cttcgtctcc gggagtatca ttcggactcc gtaccccgtt cctttcagga
      301 gaatctcgga cgtcccgcgg tgttcggcag tatccttcgg attttcaaga gattccactg
      361 ttccagccac agtaccccct ccttcggtaa aaagctcgct tctccatttt ccacctctgc
      421 ggtatgttcg gcgggaaata ccgaactccc cgggcggctt ccatcatctc ctttacggag
      481 ttttcatcct ggattcggga acgaggagat acgagagcat ctcccccacc atgggcgagg
      541 ccacggaacc tccgtggagc ccggcctcca gcatggccac agcgacatat ttcgggttat
      601 ccatcggcgc gtaccctacg aagagcgcgt gatcatcccc gtgcgcgttc tgagcggttc
      661 cggtctttcc cgcgaccgta atgccgaatc ctcccgccct ccgggccgtc cctctccgga
      721 cgacatagtc cagccccttg cgtatgacgt tcatatggct cggtgaaagg ttcagttcag
      781 ccgcctgcac cggcaaactg ttcgcgagcc gcggccggac aagcttgcct ccgttggcga
      841 tcacagcgta cattgtggcg agctggagcg gcgtcgtcag cagaaatccc tgccctatgg
      901 aataatttac ggtatcgcct ctgtaccagc cctccttgaa acgggccttc ttccagtcgg
      961 ggccggccac gacgccaccg gcctctccgg gaagatcgat tcctgtgggc ctccccaatc
     1021 cgaaaagctc aacccacttc aaaaggcggg taatcccgag cttcagtcca acctggtaaa
     1081 aatagacgtc gcaggaattc tgaagcgccc ccgtcaggtt caccgtcccg tgtcccgaac
     1141 gtctccagca gcgaaacgta cggtttccca gggtgaaagc gcccccgcag tggaaggtcg
     1201 tcgacggagt gacctccttc tctacaagag cggcaagccc gacgagtatc ttgaaggtcg
     1261 acgccggcgg gtaaagcccg gcgatactcc tgtcgagcat cggcttctgc gggtcggaga
     1321 ggagcgcatt ccactccttc gccgaaacgc cccacgagag ggggttgttg tcgtacgaag
     1381 gggaagaggc cagtacaagg acgtccccgt cgtgcacgtc catcacgatc agcgctccgc
     1441 ggtatccctg gagaagatcg gccgccaggc gctgcgcccc gagatcgagc gtcaggtgaa
     1501 ggtcctcgcc gcgccctgcc ggataggtat ctatctgccg gaccctgcgc cccctggcgt
     1561 ccacctcgac ggattctcct ccgggctccc cgcgaaggat ctcctcgtat tgcctctcaa
     1621 taccggtctt cccgatctgg tctcccccga cgtagctctt cccctccatc tgacgcagct
     1681 cgttctcgtt gatctcgccg acaaatcctg tgacgttcga cgcgatactt cccgccggat
     1741 aggtccttcg caggacgggg atcggaaaga gctgccgcgg gaactcggga tcggccacca
     1801 ggtcggccat ctgcgcgagg gttatgttgg agaccacgcg caccaccctg tagggcgccc
     1861 agaactgccg cttgacatcc cggcgaaggt cctccgccga taccggaata ccgtggcggg
     1921 agagcaaaag cgcaagattc tccaccatgt tgttcttttc gatatcaagc gggtacccca
     1981 ttatatcgaa tgtagtcacg ttcgccgcca gcgggactcc gttcctgtcg aaaatctctc
     2041 cccgtatggg ggcgaaccgg atgaaccgga ggcggttgtt cgccgcgagg cgaacgtacg
     2101 cgtcggcgtt gacgatttga aaaaagtaaa gccctcccgc gagcagaagg agcgaaaaca
     2161 taagaaacag cttccacaaa agaaggcgaa gattcatctc cggacgatgg tcagacatcc
     2221 tggtttttca acctccacgc ataggcggcg caggcaagga gcaccaacgg cacaatgtga
     2281 ctctgctgaa gcaggtacgc ccgccacact tctattcccc gcgctcccca caggaaaatt
     2341 cttccgagcg cgataggcat gttcgcggac cagaggagga aaccgaagag cgtgatcgtc
     2401 cttcccgagg ccgggatgga atgccaaatc caccggacgg acatcaatga cagaacgtaa
     2461 atcaaggagc tgatacccgg aaatccggtc catcgtaaat cccagaggac gcctccgagg
     2521 aaagcggccc aaaccacccg aactccgtcc tcggaacgaa agagcgaaag aaagacgagg
     2581 gtgagcaaaa aaatctccgg gacgaggacc ttcccggcgc cgagaaccca caaaaaatcc
     2641 tgagcatacc acgcgagcag atagatcata aaccggacct ccccccggcc ggtttctcct
     2701 gcgatggagt gcgcaggcca agaacttcga gcgcatagag ctgcgagaga gacgcgcccg
     2761 gaagaatccg ccactcggta tatccgccgc tcgtttcgcg gcgctcttcg ccgaggctgc
     2821 ccaatggaat cccgggaggc aacgcctcgc tgaccatggc ggtgctcacg cgcatcccgc
     2881 ttttgagcat ccggttctcc ggcacgtaca acagccagac tcccccttcg ccgtctcccg
     2941 ccacgacgcc gagatcccgt gtctcctcga taaccacagg aatcatcagg gatgatgacg
     3001 agagcagctc gacccacgca aaatcatcct ccacactgct gacccttccg
//
LOCUS       DAOSWW010000236         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23654_length_3049_cov_3.724783, whole genome shotgun sequence.
ACCESSION   DAOSWW010000236 DAOSWW010000000
VERSION     DAOSWW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23654_length_3049_cov_3.724783"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PK442_05845"
     CDS             complement(<1..428)
                     /locus_tag="PK442_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87206.1"
                     /translation="MAGVTFKNITISYDAKVIIDNLSLEVDDGECFAILGPSACGKTT
                     LMRALCGFKNLDSGQIYIGDKLVGTKERWKSVAPEKRNVGVVFQDYAVWPHYSVFENV
                     YYPLKKRKVPKEEAKKRALDAIEQVRMKGYEQRMPAQLSGG"
     gene            complement(613..1599)
                     /locus_tag="PK442_05850"
     CDS             complement(613..1599)
                     /locus_tag="PK442_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO87207.1"
                     /translation="MKKRVFSLLAVLVLVALMVPAFASAKEVMLYSSMKDSQLSALKD
                     AFIAKYPDIRMDYYTAGTGSVMTKLATEQQAGGIRADVIWVGDPTNYISFKEQGLLYA
                     YESPEAATMPANFKDPDNCYVAGRVIMLGFVYNTSALSANDVPKTWKELAEFKGYTAM
                     TDPTFSGTTLYTVAGLVQHPDFGWDFLQKLKDKGMLLERGSSAVVTKVGSGEYDVSVG
                     VDYIARTMIAQGSPIAFAYPQDGIPIILSPIAIVKNTANIEEAKLLFDFILSLEGQGI
                     LMKEYSAPVRPELSLEGALSVEEAAKKILPVDDNRLVAEKDELLKKFDSIFK"
     gene            1936..2859
                     /locus_tag="PK442_05855"
     CDS             1936..2859
                     /locus_tag="PK442_05855"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOO87208.1"
                     /translation="MKIAVFGIGGVGGIVGGALAKGHPDTYFYVRGDNLDAIRRNGLK
                     VESAVFGDFTVRPKLASDKASELGAMDVVFVSCKGYGLKAACEAISPMVRPDTVVIPL
                     LNGVVVSDLMEPLLPPCILADGTIRVFSRLEKPGHIVQSTEMCSIVFGMKDGSRPAVL
                     DRIAAVLNKAGLKTTVSENILVDSWSKYVMMCGNSVLFCYYDGPAGKVREDPQYRKVF
                     DAVTGELIAVAAAKGVVLPAETAEKVAAAFSNFPPDAMSSLYRDLSGGKPARETELDH
                     IIGRMVEMGRQTGVPTPYHTIAYERFASRQD"
BASE COUNT          645 a          906 c          850 g          648 t
ORIGIN      
        1 ccccccgaga gctgggcggg catacgctgc tcgtacccct tcatgcgcac ctgctcgatc
       61 gcgtctagcg ctctcttttt tgcctcctcc ttcggaacct tccgcttctt cagggggtag
      121 tagacgttct cgaacacgct gtagtggggc cacaccgcat agtcctgaaa aaccaccccg
      181 acgttgcgct tctccggggc gacggacttc caccgttcct tggttccgac cagcttgtcg
      241 ccgatgtaga tctgccccga atccagattc ttgaagccgc aaagcgcccg catcagcgtg
      301 gtcttcccgc aggcggacgg accgaggatg gcaaagcact ccccgtcgtc gacctccagc
      361 gacaagttgt cgatgatgac ctttgcatcg tagctgatgg tgatgttttt aaatgttact
      421 ccggccatta cgttcacctc gcactgcttt ccaaaaaatg catcgaaaag cgggtataaa
      481 aaagcggccg gacaatctcc gggagaacgc ctgaaaggcc gcttcccaaa tattcctccg
      541 gccggcgacg ccgcttcgat ggtactgaag gggaagggcg tttcaggccc ctccccgcga
      601 aactctgtcg acctacttga aaatgctgtc gaacttcttc agcagctcgt ccttctccgc
      661 caccaggcga ttgtcgtcca cgggaagaat cttcttcgcc gcctcttcca cggagagggc
      721 cccttcgagg ctcagctccg gacggacggg ggcgctgtac tccttcatca ggattccctg
      781 accttcaagg gagaggatga agtcgaagag cagcttcgct tcctcaatgt tcgcggtgtt
      841 cttgacgatc gcgatggggc tcaggatgat gggaatcccg tcctgaggat aggcgaacgc
      901 gatgggagag ccctgggcta tcatggtgcg ggcgatatag tccacgccca cgctcacgtc
      961 gtactcgccg gaaccgacct tcgtcaccac cgcgctggag cctctctcaa gcagcatacc
     1021 tttgtccttc agcttctgca ggaagtccca accgaaatcg ggatgctgca ccagtccggc
     1081 gaccgtgtag agcgttgtgc cggagaaggt cgggtcggtc atcgcggtgt accccttgaa
     1141 ctccgcgagc tccttccagg tcttgggcac gtcgttggcg gaaagcgccg acgtgttgta
     1201 cacaaagccc agcatgatta cacgtcccgc aacgtagcag ttgtccggat ccttgaagtt
     1261 cgcgggcatg gttgccgcct cgggagactc gtacgcgtac agaagcccct gctctttgaa
     1321 ggagatatag ttcgtcggat ctcccaccca gatgacgtcg gcccggatgc ctccggcctg
     1381 ctgctcggtg gccagcttcg tcatcacgct gccggtaccc gcggtatagt agtccatacg
     1441 gatatccgga tacttcgcga taaaggcgtc tttcaacgcg gacagctgag agtccttcat
     1501 cgaagagtac agcatcacct ctttcgccga agcgaacgcg ggaaccatca gcgcaacaag
     1561 tacaagaacc gccagaagac taaaaacacg ctttttcatt gtattgcccc ctttgtgtat
     1621 aaaatgcttg gcaacgcggc aaagagccct tttcgacccc ttgcgtacat gcgtgcgttg
     1681 ccctgttaca gaattgtaac attcacacgc cccgtttgcc agtgttgctg gagtattgat
     1741 gcaacggagg tcctttcgcc gccgtccgcg gcgtcgctca cacatgcttt tcccggctcc
     1801 gtgcgtcgtg ttgctcccgt atggaaactg atagggcgcg gtcccgattt gatccgagga
     1861 agaaaacggc atatactgaa agccggaaaa gcgcgcttca cgcgcttact tgcagaactt
     1921 gggaggtgtc atactatgaa aatcgcagtg ttcggaatag gcggggtcgg cggaatcgtc
     1981 ggcggcgcgc tggcgaaagg ccatcccgat acctatttct acgttcgcgg agacaacctc
     2041 gacgccattc gcaggaacgg actgaaagtg gaatccgctg tcttcggcga ttttaccgtt
     2101 cgcccgaaac tcgcctcgga caaggcttcg gagctcggcg ccatggacgt cgtttttgtt
     2161 tcctgcaagg ggtacggcct gaaagcggcc tgcgaggcga tctccccgat ggtgcggccg
     2221 gataccgtcg tgatccctct gctcaacgga gtcgtcgtct ccgacctgat ggagccgctg
     2281 ctgcccccct gcatcctcgc cgacgggacg atccgcgtct tcagccgtct ggagaagccg
     2341 gggcacatcg tccaaagcac ggaaatgtgc agcatcgtct tcggcatgaa ggacggaagc
     2401 agacccgccg tattggaccg aatcgcggct gtactcaaca aggctggctt aaagacgacg
     2461 gtatcggaaa atatactggt ggacagctgg tccaagtacg tcatgatgtg cggcaacagc
     2521 gtgctgttct gctactacga cggcccggcg ggcaaggtgc gcgaggaccc gcagtatcga
     2581 aaagtgttcg acgccgtaac gggcgaactg atcgcggtgg ccgccgccaa gggggtcgtc
     2641 ctgcccgcgg aaacggcgga aaaagtcgcg gccgcttttt ctaatttccc gccggacgcc
     2701 atgtcctccc tctaccgcga cctcagcggc ggcaaaccgg cgcgcgaaac cgaactcgac
     2761 catatcatcg gaagaatggt ggagatggga cggcagacag gcgttcccac cccgtaccac
     2821 acgattgcct acgaacggtt cgcctcacgg caggattaac gcccgaaagc cgagaacaaa
     2881 gagaagggcc ctaagaaaac aattttctcg gactcctttt tttggggaac agagcagcgc
     2941 gaaatattga tctgcaggga tttttcgatg aatttaaaaa ctctccggat actcttcatt
     3001 tccgtgacaa atattattaa cacgtgttat aatcttgacg aaaggaatg
//
LOCUS       DAOSWW010000237         3036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23819_length_3036_cov_4.102650, whole genome shotgun sequence.
ACCESSION   DAOSWW010000237 DAOSWW010000000
VERSION     DAOSWW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23819_length_3036_cov_4.102650"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="PK442_05860"
     CDS             complement(<1..963)
                     /locus_tag="PK442_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOO87209.1"
                     /translation="MSTYISQEQKLKIQDKFKEYLKKEHPEWVERTVYTNVSDSFYVF
                     NNDIGMNPWSCFVSDALMEEAREVLTEYFRVHDKPGNPETRANGYIKSMQLLKLFLDR
                     NYPGLASEQSAHTHAPSCWIFQCNPKYYDLLRALREKETLRWSINQYAKRIQAGDKVY
                     LWMSGSDGGIVASGKVDSAPEKRAPDLNDAYWMDGYRPTEEYLCVDISLERKFADSVI
                     SRSVLSADERTKKLGILLFPQGTNFHVAREQEAVIESILEGAYVRVPATSDDATAEGK
                     RYWMYAPGKGAEKWDEFHKHGIMAVGWDELGDLSRYSSKQNIKEK"
     gene            complement(1194..1613)
                     /locus_tag="PK442_05865"
     CDS             complement(1194..1613)
                     /locus_tag="PK442_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOO87210.1"
                     /translation="MSVRFVVDTSVVMSWCFEDEGNGYAETVLESLESAEAIAPAIWP
                     LEVGNVLLVAERKKRLGQAASVRFLSLLGALPITVEQEPSERMLKEILSLARTLGLST
                     YDASYLDLAMRLDLPLATLDAALANAAKKSGVQIYRP"
     gene            complement(1613..1852)
                     /locus_tag="PK442_05870"
     CDS             complement(1613..1852)
                     /locus_tag="PK442_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOO87211.1"
                     /translation="MEKIGVYEAKTHLTQLMDRVSKGEKVTITKHGVPVAILQPADFS
                     KTEPVREIIEQLKQFRSGRRLDGLSIRDMIEEGRH"
     gene            2062..2391
                     /locus_tag="PK442_05875"
     CDS             2062..2391
                     /locus_tag="PK442_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOO87212.1"
                     /translation="MVNATVNIRLEAEDKKEAEKIFATLGLTTSEAIRLFYRQTILRR
                     GLPFSVDIPNDVTVAASWKPAVSKIWQLVCRLPLRGLYDRLGDGKNHDAVVRGETRVR
                     TPLARSC"
     gene            2476..2748
                     /locus_tag="PK442_05880"
     CDS             2476..2748
                     /locus_tag="PK442_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOO87213.1"
                     /translation="MPVVVNATEFKQRFGAYVEHAQKEPVEITRSGRSSAVLLSADEF
                     RRMREHEDKYWAMLAEEAKRGGFAGTEESAEWLAEGMRRCDRHEEA"
     gene            2735..2995
                     /locus_tag="PK442_05885"
     CDS             2735..2995
                     /locus_tag="PK442_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO87214.1"
                     /translation="MRKLNLTKDFMKFCDVLPPKQFKQVVAKTLALASNPRPHDSIEL
                     KGSSGQFRVDIGEYRVIYEFDKEEVRVCVIDKRNDGQVYRSG"
BASE COUNT          648 a          803 c          844 g          741 t
ORIGIN      
        1 tttctccttg atgttttgtt tggaggagta ccgagaaaga tcgcccagtt cgtcccaacc
       61 gaccgccatg atcccgtgtt tatggaattc atcccacttc tccgctcctt tgcctggagc
      121 gtacatccag taccgcttcc cttccgcggt ggcgtcgtcg ctcgttgccg gtacacgaac
      181 ataggcgcct tccagtatgc tctcgatgac ggcctcctgc tcccgagcga cgtgaaagtt
      241 cgttccctgc gggaagagca ggatccccag ttttttcgtt cgctcgtcgg cggaaagaac
      301 gcttctggaa atgacagagt cggcgaactt gcgttccaaa gaaatgtcga cacacagata
      361 ttcctcggtc gggcgataac cgtccatcca gtaagcgtcg ttcaaatcgg gcgccctttt
      421 ctccggagct gaatctacct tccccgaggc gacgatgcct ccgtcggaac cggacatcca
      481 gagatacacc ttgtctccgg cctgaatccg tttcgcgtac tgattgatgc tccagcggag
      541 cgtctccttt tcccgcaggg cccgaagaag gtcgtagtat ttggggttgc actggaatat
      601 ccagcaggag ggggcgtgcg tgtgcgctga ctgttcggag gcgaggccgg ggtagttgcg
      661 atcaaggaac agcttgagga gttgcatgga tttgatatag ccgttcgcgc gtgtttcggg
      721 atttcccggc ttgtcatgta ctcgaaaata ctccgtgagc acttcccggg cctcttccat
      781 caaggcgtcg ctgacgaagc aggaccaagg gttcatcccg atatcgttat tgaagacata
      841 gaaggagtcg ctgacgttcg tatacactgt tctttcaacc cattccggat gttctttctt
      901 cagatattct ttgaacttgt cttggatttt cagcttttgc tcctgtgaga tataggtgct
      961 catacgtgtg cctccctcat ttaaggcgaa gcgctcccgt aaacgccggc gatgccctga
     1021 tttttccgta tccggcattg gtatcccgac ctgtttttca tcatgtgagg agctgtattg
     1081 agacgctcgc agaatgcaac ggagagcctc gttttccaca gtccaatata tacgttgaag
     1141 tcttattgtc aagtatttcg tccggcctcc gcttccggga gcactcttcg ccgttacggc
     1201 ctgtagatct gtactccgct tttcttcgcc gcattcgcga gggcagcgtc cagcgtggcg
     1261 agcggaagat cgagccgcat ggcaagatcc aggtaggatg cgtcgtaggt ggacagtccg
     1321 agggtacggg cgagcgaaag gatttctttc aacatgcgtt cggagggttc ctgttccaca
     1381 gtgatcggca gcgcgcccag gagggagaga aagcgaacgg acgcagcctg accgagtcgt
     1441 tttttccttt cagccacaag cagcacattc cctacttcaa gaggccagat cgcgggtgcg
     1501 atggcctccg ccgattccag actttcgaga acggtttccg catacccgtt cccttcgtcc
     1561 tcgaagcacc atgacatgac gacggaggta tccaccacga agcgcacgga catcagtgcc
     1621 ttccctcctc gatcatgtcg cggatggaga gtccgtcgag ccggcggccg cttcggaatt
     1681 gcttcagttg ttcgatgatc tcccgtacgg gttccgtctt cgaaaagtca gcgggctgca
     1741 aaatcgccac gggcacgccg tgcttcgtga ttgtaacttt ttcacccttg gaaacgcgat
     1801 ccataagttg cgtgagatgt gttttcgcct cgtagactcc gattttttcc atattctccc
     1861 ctccgtttaa accagtttaa gaccagtcta tatcataccg tcggaaaatt caagagagtg
     1921 agtctcactg tagagactat ttctggattt cctcgaggtt ttgatcatcg gaaaaagaag
     1981 actgccagcg acgccagtat cgggtatcac ccttgagttg taacgcaaaa agcaatacaa
     2041 tgtgtcctgg gggtgagcgg tatggtgaat gcgaccgtaa atatcagatt ggaagccgag
     2101 gataagaaag aggccgagaa gatctttgca acactcggct tgacgacgag tgaagcgatt
     2161 cgtcttttct acaggcagac gattctgcgc cgggggcttc ctttttcagt cgatatcccc
     2221 aacgacgtca ccgtcgcggc gagctggaaa ccggcagttt cgaagatctg gcaactcgtt
     2281 tgtcggttac ctctgcgcgg cctctatgat cgccttggcg acggcaagaa tcatgatgct
     2341 gtcgtccgtg gtgaaacccg tgtccgcacc ccccttgctc ggtcctgttg acgtaccccc
     2401 ttcgaaagag tatccttgta gaggcgaaat gatcgttatg atcgttttaa tcataataat
     2461 taagaggtga gtagaatgcc cgttgtggtg aatgcgacgg aattcaagca gcgtttcggc
     2521 gcgtatgtcg agcatgctca aaaggagcct gtcgagatca ctcgttccgg ccgttcttcg
     2581 gctgtgctgc tctccgccga cgaattccgt cgaatgcggg agcatgaaga caagtattgg
     2641 gctatgcttg cggaggaggc gaagcgcggc ggtttcgccg gaacggagga gtccgcggag
     2701 tggctggccg agggaatgcg ccgttgtgac cggcatgagg aagcttaacc tgacaaagga
     2761 cttcatgaag ttctgcgacg tccttccgcc gaaacagttc aaacaggtcg tggcgaagac
     2821 tttggcgctc gcatcgaacc ctcgcccgca cgacagcatc gagctgaagg gatcctcagg
     2881 gcagtttcgc gtcgacatcg gtgaatatcg agtaatctac gagttcgaca aggaggaagt
     2941 gcgcgtctgc gttatagaca aaaggaacga cggacaggtg tacaggagcg gataggatag
     3001 tcctgcggaa tgaaaagcgg tccgctcata cagcgt
//
LOCUS       DAOSWW010000238         3033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23863_length_3033_cov_2.840497, whole genome shotgun sequence.
ACCESSION   DAOSWW010000238 DAOSWW010000000
VERSION     DAOSWW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23863_length_3033_cov_2.840497"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..2112)
                     /locus_tag="PK442_05890"
     CDS             complement(181..2112)
                     /locus_tag="PK442_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO87215.1"
                     /translation="MKKTAFSLTRKLVFLCLSAILFLSCASCGLAAEKEERARWVPGE
                     VIVTYRGSGAQNTLQQRASSADGGSARVFGSLSSASGNTVAVLRIPGKSTEELLKEFS
                     ADPNMISISPNYVIRLLLRTPNDPRFSEQWGLYNTGQSGGTGGADISAPQAWDLRTSA
                     LSDRVVAILDTGIAKNHEDLRANLWSGPGGSHGYDFVQNDDDPEDDHGHGTHVAGIIG
                     AVGDNGSGVAGVAWNVKLMAVKMLDADGYGTSAMELAAYNWVLARKQAGVNIVAINAS
                     FGDSNPVQASKDAIETLGRNGILFIAAAGNDGANSDSTPSYPASYGLTNIISVAATDK
                     NDRLTSYSNYGKNTVHLGAPGGDTGGGAGAILSTWTGYTPRAGDLFFDDMEQGKGNWI
                     TAAEGASQNLWDIIDTTRSGVPTKAWSDSPGRHYPHNQRTFLRLNRDIDLSGTFGESI
                     MFGMQVRLDLESGSDFLVLQFSADGGATWRDMHSFTGGGDTWRAFITSVPPAFRTNRF
                     RFGFRFESDSSNLIFRYAGVQIDDTGVGRAETSSYNRIAGTSMATPFVTGATALVASH
                     FPNDGAATIRSRILNSVDKIDSLNNKLATGGRLNLYKALQTDSDIGDEDGVGGGCSLS
                     SSPFALLLLLPLFVFRRRR"
     gene            complement(2203..>3033)
                     /locus_tag="PK442_05895"
     CDS             complement(2203..>3033)
                     /locus_tag="PK442_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOO87216.1"
                     /translation="LTKRFLPAFFILCVLLVGCAFAASSEQPKTNLPFTVIAADVDEV
                     IIYEGDDEEVQPLYAVLMGVGEERAIKGLVSGDNVTTQRIRWSIDNWGVARIVGQENE
                     GDVIEANEIAVEAWGNGTATIIATSVQDPTISEFCTVYVQNSPVPVEEVTIDKEEIGI
                     LSGYFGELTASVLPPQADFRHISWHSSNPEIVAIDPTMRTGNRTRLLTAGLHTGTAII
                     SAVSNYSGGRGGERPYATCMVHHYVEGPRSTEGGCSAGSAPGLLGLLLLAPLLIFLKK
                     "
BASE COUNT          601 a          889 c          867 g          676 t
ORIGIN      
        1 catcgtgacg agcgctcttt cgagcccatg ggaggcggtt gccttccctt tgccagagaa
       61 gcccgttctt tttacctcgg tcaactgaca tcccgacctg cttcaaagag gacaagtaac
      121 aagagggcgt cccgcagttt ttctggacgc cctcttgttg tacattgtga cgcgtagcag
      181 ctaccttctt cgccgaaata cgaacagcgg cagaagcaac agcagtgcga agggggacga
      241 cgagagggag cagcctcctc cgacaccgtc ctcgtcgcca atgtccgaat cggtctggag
      301 cgccttatac agattcaggc gtcctccagt agcaagcttg ttgttcagcg agtcgatttt
      361 atcgacgctg ttcaggatac gggagcggat cgtggccgca ccgtcgttcg ggaagtggga
      421 ggccacgagg gctgtcgctc ccgtgacgaa gggcgtcgcc attgacgtac ccgcgatccg
      481 gttataggaa gacgtctccg ctctgccgac gcccgtgtcg tcgatctgta cccctgcata
      541 gcggaagatc aggttgctcg agtcgctttc aaagcggaag ccgaagcgga atctgttcgt
      601 tctgaaggcg ggcggcaccg aagtgatgaa tgccctccac gtatcaccac ctccggtgaa
      661 gctgtgcatg tcgcgccacg tcgcgccccc gtcggcggaa aactgaagga ccagaaaatc
      721 cgagccgctt tcgaggtcga gacgcacctg cataccgaac atgatggact ccccgaaagt
      781 gccggagagg tcgatgtccc tgttcaggcg gagaaacgtt cgctggttgt gcggatagtg
      841 ccttcccgga ctgtccgacc acgccttcgt cggcacgccg gaccgcgtcg tgtcgatgat
      901 atcccagaga ttctgcgagg cgccctcggc ggcggtgatc cagtttccct tgccctgctc
      961 catgtcgtcg aagaaaagat cgcccgctcg cggcgtatag cctgtccagg tgctgaggat
     1021 ggcccccgcg cctcctccgg tatcgcctcc cggcgcgccc agatggacag tgtttttccc
     1081 gtagttcgag tagctcgtca gcctgtcgtt cttgtccgtc gcggccacgg aaatgatgtt
     1141 ggttaggccg tacgatgccg gatagcttgg agtgctgtcg ctgtttgctc cgtcgttccc
     1201 tgcggcggcg atgaagagaa tgccgttgcg gccgagggtt tcgatggcgt ccttgcttgc
     1261 ctgcacaggg ttggaatctc cgaacgaggc attgatcgcc acgatgttga ctcccgcctg
     1321 tttcctagcc agcacccagt tgtaggccgc cagctccatt gcgctggtgc cgtagccgtc
     1381 ggcgtcgagc atcttcaccg ccatcagctt cacgttccac gcgactcctg cgacgccact
     1441 gccgttgtcg ccgacagcgc cgatgatgcc cgctacatgc gtgccgtgcc cgtgatcgtc
     1501 ctccggatcg tcgtcgttct ggacgaagtc gtatccgtga cttccaccgg gaccggacca
     1561 caagttggcg cggagatcct cgtgattctt cgcgatgccc gtgtcgagga tggcgacaac
     1621 acggtcggag agagccgatg ttcgcaaatc ccaggcttgc ggggcggaga tgtccgctcc
     1681 gccggttcct ccgctctgcc cagtgttgta caacccccac tgctcggaga aacggggatc
     1741 gtttggcgtg cgcaacaaca ggcggatgac atagttcggg gagatgctga tcatgttcgg
     1801 gtcggctgaa aactccttca gcagctcctc agtggacttt ccaggaatac ggagcacggc
     1861 gacggtattt ccgcttgccg aggagagcga tccaaaaacg cgcgcgctcc cgccgtccgc
     1921 tgaagatgcg cgttgttgca aagtattctg cgctccgctc cccctgtagg tgacaatgac
     1981 ttcgcctgga acccatctgg cgcgttcttc cttttcggcg gcaagaccgc atgaagcaca
     2041 tgacaaaaag agaatcgctg aaaggcaaag aaaaacgagc ttcctcgtca atgagaatgc
     2101 tgtttttttc atgatgtatt cacctctttc cgctgtgatc aatcatacct tctttatgtg
     2161 aaaaagggcc ccccgtatga agggaggccc tcggaaaacg ggttacttct tcaagaaaat
     2221 gagcagaggc gccaggagca gaagaccgag aagccccgga gcgctccccg cgctgcaacc
     2281 gccctcggtc gaacgggggc cttccacgta gtgatggacc atgcatgttg catagggacg
     2341 ttcgccgccg cgtcctccgc tgtaattcga gacggcggag ataattgctg tacctgtgtg
     2401 gagtcctgca gtcaaaaggc gtgtgcggtt acctgttcgc attgtagggt ctatagctac
     2461 aatttccgga ttgctcgaat gccaggaaat atgacgaaaa tctgcttgag gcggcaagac
     2521 gctcgctgtc agttctccga agtagccgct gaggataccg atctcttcct tgtcgatggt
     2581 gacttcctct accgggactg gcgaattttg cacgtacacg gtgcaaaatt cggaaatagt
     2641 gggatcctga acggaagttg caattatggt ggcagttccg tttccccacg cttccaccgc
     2701 gatctcgttt gcttcgatca cgtcgccttc gttctcctgt ccgacaatcc ttgcgactcc
     2761 ccagttatcg atagaccagc gtatgcgctg tgtcgttaca ttgtctcccg atacaagccc
     2821 cttgatcgcc cgctcttccc cgacgcccat cagaacagcg tagagaggtt gtacttcttc
     2881 gtcgtctccc tcgtagatga taacttcgtc aacatcggcg gcaattactg tgaaagggag
     2941 gtttgtcttt ggctgttccg aggaagccgc gaatgcacag ccgaccaaca gcacgcagag
     3001 gataaaaaac gcaggaagaa acctttttgt cag
//
LOCUS       DAOSWW010000239         3030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23892_length_3030_cov_4.430588, whole genome shotgun sequence.
ACCESSION   DAOSWW010000239 DAOSWW010000000
VERSION     DAOSWW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23892_length_3030_cov_4.430588"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..675
                     /locus_tag="PK442_05900"
     CDS             148..675
                     /locus_tag="PK442_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOO87217.1"
                     /translation="MRSVAEKRESREAVCRKLDEMGISYEMIEHEAAYTVEDMDRVGF
                     DEKTTVCKNLFVRDQKKKATHWLVVLLKDKRADLDNLAEQLGTKHLSFASADRLKKYL
                     GLAQGEVTPLGVLNDAAHEVIVVFDKDLIGRERLGVHPNDNSATLLLSFGDIKRFVEQ
                     NGNTVRYVTLKAETA"
     gene            complement(796..1521)
                     /locus_tag="PK442_05905"
     CDS             complement(796..1521)
                     /locus_tag="PK442_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOO87218.1"
                     /translation="MLLRRERELVVEHCRMLATHKLTKGTGGNISALDREKRLFAISP
                     SGMDYFAMQPEDVVVLDLDGKTVDGERRPSSEMDMHRLLYVDREDINAVVHAHSTYAT
                     TLACLHWSLPALHYLIGYAVSRGPTNTSEASKESCQSLSAHASSARGDGVRCTPYAPF
                     GSQELAEIARDGMKGRYAVLLGNHGLLAAGPNMQYAFNAAEEIEFVCELYWRAKSVGD
                     PVILSEEDMNVVLEKFKTYGQRA"
     gene            complement(1555..>3030)
                     /locus_tag="PK442_05910"
     CDS             complement(1555..>3030)
                     /locus_tag="PK442_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HOO87219.1"
                     /translation="GRDFYAIVSIDRAGKNPEQEPFNVRFELYRDGVLAPLRTVTSTV
                     DDGTGLTPLNAIKTDYPHGWTPGTALDILASPPPDLVYNPALPVSFYDAAIKAVVTRH
                     YAAALIQGGHTKTFDSNYAAIYTRDLEEGAYTLKATAVGAAGAELATVSVPLTFGSVP
                     DKIISRFSPENHMENVTAFAQKNNSHIYNDLFPGYWDASSLPHGGIPGTLFYEIVRRW
                     RPNDLLEYMNGTIRAVVYNVHVSSATQSVELGGLAYAGRLGSNSILWYHYDSGEVSLA
                     YDLGNGQAASKLGTIVPFPDGKRLVLVRAEIRGDGQTEPPTSDYVYTPELADKTVDWN
                     VADGVAVKPKQLLSLFGVVKPIQPALNDVVANDDGTYTINNRIATVRYTLLDGTQEML
                     TFDAKRVELTRNGINNRPSIYEFRHDIPVPATFNKPWTVRVAAFDIYGTLVPGTEMSF
                     TLAPYSPGGGGGGGGCSVGAAPFALLLLLPLLVLAGKRGRT"
BASE COUNT          571 a          930 c          935 g          594 t
ORIGIN      
        1 tcaagagcgc gcgccccaca ccccgcttca tttcggacaa tcgtttcttc gtttgacctg
       61 acggcctttt cgggtatcat agacgcggtt ctttcagcag gaatagaagc aacaacctga
      121 gatctctcaa acaaggaagg tgctgcaatg cgaagcgtcg ccgagaagcg tgaatcacgg
      181 gaagcggtct gccggaagct cgatgaaatg ggcatatcct acgagatgat cgagcacgag
      241 gcggcctaca ccgtcgaaga catggaccgc gtggggttcg acgagaagac gaccgtctgc
      301 aaaaacctct tcgtccgcga ccagaagaaa aaggcgaccc actggctcgt cgtgctgctc
      361 aaggacaagc gggccgacct ggacaacctg gcggaacaac tggggacgaa gcacctcagc
      421 ttcgcctccg cggaccgcct caagaaatac ctcggcctcg cccagggcga ggtcaccccc
      481 ctcggcgtgc tcaacgacgc agcccacgag gtcatcgtcg tcttcgacaa ggaccttata
      541 ggcagggagc gtctgggcgt ccacccgaac gacaacagcg cgacgctgct cctgtcgttc
      601 ggggacatca agcggttcgt cgagcagaac ggcaatactg tgcggtacgt tacgttgaaa
      661 gcggagacgg cgtagcgcgc aacgaaaaac cccgcttcgg aacctgcccg ccgcattcct
      721 ccgttctccg gaacaaacaa accgggagcc cgaaattcag ggctcccggt tttcatgcgt
      781 atttttccga acggttcagg cccgctggcc gtaggtcttg aacttctcca gcacgacgtt
      841 catgtcctct tcgctcagta tgacggggtc gcccacgctc ttcgcgcgcc agtagagctc
      901 gcagacgaat tcgatctcct cggcggcgtt gaaggcgtac tgcatgttcg gcccggcggc
      961 gaggaggccg tgattgccca gaaggacggc gtaccgcccc ttcatcccgt cgcgcgcgat
     1021 ctccgccagc tcctgcgatc cgaacggcgc gtagggggtg caccggacgc cgtctcctct
     1081 agcgctagat gcatgcgcac taagcgactg acacgactcc ttactcgctt cgctcgtgtt
     1141 cgtcggtcct ctgcttacag cgtatccgat caggtagtgg agcgccggaa ggctccagtg
     1201 gaggcaggcg agcgtcgttg cgtaggtgga gtgcgcgtgg acgacggcgt tgatgtcctc
     1261 cctgtccacg tagagcagcc ggtgcatatc catctcgctc gacggccttc tctccccgtc
     1321 gacggtcttc ccgtcgaggt cgaggacgac gacgtcctcc ggctgcatcg cgaaatagtc
     1381 catcccgctc gggctgatcg cgaagagcct cttctccctg tccagcgcgc tgatgttgcc
     1441 gccggtcccc ttggtcagct tatgggtcgc caacatcctg cagtgttcca caacaagttc
     1501 acgctcacgc cggagcagca ttcgtcgttc ctccattgca atacaagcgt aggcctaggt
     1561 ccttccgcgc tttcccgcaa gaacaagcaa cggcagaagc agcagcagcg cgaacggcgc
     1621 ggcgccgacc gagcatccgc cgcccccgcc gccgcctccg ggcgagtagg gagcgagcgt
     1681 gaagctcatc tccgtacccg gcacgagcgt tccgtagatg tcgaacgccg cgacgcggac
     1741 cgtccacggt ttgttgaagg tcgccggaac aggaatgtca tgccgaaatt cgtagatgga
     1801 cggtcggttg ttgattccgt tgcgggtcag ctcgacgcgt ttggcgtcaa atgtaagcat
     1861 ctcctgcgtt ccgtcgagca gggtgtagcg gactgtcgcg atcctgttgt tgatggtgta
     1921 ggttccgtcg tcgttggcaa ccacgtcgtt aagggcgggt tgaatgggct tcaccacgcc
     1981 gaaaaggctg agaagctgct tcggcttcac cgcgacgccg tcggccacgt tccagtccac
     2041 cgtcttgtcg gcaagctccg gagtatagac atagtccgac gtcggaggtt cggtctgtcc
     2101 gtcgccgcgt atctccgcgc ggactaggac aaggcgcttg ccatccggga aggggacgat
     2161 ggttcccagt ttggacgccg cctggccgtt cccgagatcg taggcgagcg acacctcccc
     2221 gctgtcgtag tgataccaga ggatcgaatt ggaacctagc ctgcccgcat acgccaatcc
     2281 gccgagttcg accgattggg tcgctgaaga gacgtggacg ttgtacacga cggccctgat
     2341 tgtgccgttc atgtactcca gcaggtcgtt cggacgccag cgccgcacga tctcgtagaa
     2401 gagcgtcccc ggtatgcctc cgtggggcag agaggatgcg tcccagtatc cggggaagag
     2461 atcgttgtag atgtgcgagt tgttcttttg cgcgaaggcc gttacatttt ccatgtggtt
     2521 ctcgggagaa aagcgggaga tgatcttgtc cggcacgctc ccgaaggtca gaggtacgct
     2581 gacggtggcc aactctgcgc ccgctgcgcc gacggccgtc gccttgaggg tgtaggctcc
     2641 ctcctccaga tcgcgagtgt atatggccgc gtagttgctg tcgaaggtct tcgtatggcc
     2701 tccctggatg agggcggcgg cgtagtggcg cgtcaccacg gccttgatcg ccgcatcgta
     2761 aaaggaaaca ggcagcgccg gattatagac gaggtcggga ggcggcgaag cgaggatgtc
     2821 cagcgcggtt cccggagtcc atccgtgcgg atagtccgtc ttgatagcgt ttaacggcgt
     2881 caaccccgta ccgtcgtcca ccgtgctcgt cactgtgcgc agcggggcga gcaccccgtc
     2941 gcggtacagc tcgaatcgga cgttgaaggg ctcctgctcg ggatttttgc ccgcgcgatc
     3001 gatggagacg atcgcgtaga agtcgcgtcc
//
LOCUS       DAOSWW010000240         3025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_23949_length_3025_cov_3.874074, whole genome shotgun sequence.
ACCESSION   DAOSWW010000240 DAOSWW010000000
VERSION     DAOSWW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23949_length_3025_cov_3.874074"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..616
                     /locus_tag="PK442_05915"
     CDS             293..616
                     /locus_tag="PK442_05915"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOO87220.1"
                     /translation="MDVFKAVDMAVKGNIYEGGKVTSRTFWTADGERKTVGIMLPGSY
                     SFSTSTQEKMEMTNGIVEVRLDPDGEWSRYKEGEFFMVPAGVTFEIRCAGVAEYVCSY
                     LNESK"
     gene            647..>3025
                     /locus_tag="PK442_05920"
     CDS             647..>3025
                     /locus_tag="PK442_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO87221.1"
                     /translation="MAFRKMWLKINGANRIFICDPEKDSLADVPRRLGLTSVKIGCGT
                     GVCGSCSVILDGALVRSCTRKIGKIPEYSEVITLEGIGTPDHLHPLQVAFMKLGAVQC
                     GFCTPGFIVSSYALFLENKNPTRKEVRTWFQKHKNVCRCTGYKQIVDAVMEAAKVVRG
                     EARIDEIGPSYSTEDGYYGKPLVRPTALAKVCGLADYGDDQELKMPAETLHVAMVQPR
                     VAHHAKILDIDVSEAEKMPGVAKIILAADIRAAGGTNIMAEGQMHERTTVMAPSREVL
                     NEKKIFRYGDVVALAVADTKDHARAAAAKVKVTIEQLPEYLNFLDAVMPDAMRIHEDT
                     PNIFCIQPLLKGAGLEDASKVAGIIDSSPYSAEGSFYSARQPHLSIEGDSIQAYFDED
                     GYLTFQCKSQGVYSSIGRIGNSIGMPKDKLRVVMNPTGASFGWSTNAGDLCLAGAAAM
                     VMKAPVSLSMSYEEHQHFSGKRCPCHSNGRAACDENGKITAVEFDFGMDHGAYSWGGD
                     DIVTKQPRFAFFPYYVPHAAGLARIANTNHTFGTAYRSYGSPQAYTLSEALMDMLAEK
                     AGIDPFEFRWRNIAREGQTNINSYPFRQYPMEEMMKKMRPHYERAVAEAKAADTPEKR
                     RGVGISWGGFNVTEGGTDKATVAIELNPDGTFTKYDTYQELGQGGDIGSLMLTLEALK
                     PLGVTPEQIKQVQNDTKLCPDAGMSGASRTHFMSGNATIIAAGKLIAAMKKPDGTYRT
                     YEEMKAEGLETKYFGAYSNTSVPGLCRLDPNTGIGDPTPAFTYCLNLAEVEVD"
BASE COUNT          688 a          942 c          894 g          501 t
ORIGIN      
        1 tcgccggggc cattatactc catttccctc tgcattgcca acaaaaaaac atttttctga
       61 acgcatccgt ctctttacac acccctctcc ggaacgcctc cccgccttgg cgccggacac
      121 actacggggg aaggcgcgcg ttgtcataag tgagcaaaac cgaccaaaaa gaaacacaag
      181 cagtcacaaa tatcaaaata ttgcatatct ccccgtactc ttcatatact gagtggacta
      241 tgaagcgccc ctctcttccg gaggcgcgcc tatccgagag gagctggttc atgtggatgt
      301 gttcaaggcg gtcgacatgg cggtgaaagg gaacatctac gagggcggga aggtcaccag
      361 cagaaccttc tggacagccg acggggaacg gaagacggtc gggatcatgc tgccggggag
      421 ctattcgttc tcaacctcga cgcaggagaa gatggagatg acgaacggca tcgtcgaggt
      481 ccggctcgat ccggacggag aatggtccag gtacaaagag ggagagttct tcatggttcc
      541 ggcgggtgtc acgttcgaga tccgctgcgc cggggtcgcc gaatacgtgt gcagttatct
      601 gaatgagagc aagtagtacg gaagaaacca taaagggggt accgctgtgg cttttcgaaa
      661 gatgtggctc aagatcaatg gcgcgaaccg gatattcatc tgcgatcccg agaaggactc
      721 tctggcggat gtgcctcgcc ggctcggcct gacgagcgtc aagatcggat gcgggaccgg
      781 cgtctgcggc tcctgttccg tgatcctcga cggagcgctc gtgcgttcct gcaccagaaa
      841 gatcggcaag atccccgagt acagtgaagt catcacgctc gaaggaatcg gaacgccgga
      901 tcacctgcac cctctgcagg tcgccttcat gaagctgggc gcggtacagt gcggcttctg
      961 tactccggga ttcatcgtct cgtcctacgc gctctttctc gagaacaaaa acccgacgcg
     1021 caaagaggtc cggacatggt tccagaagca taagaacgtc tgccgctgca caggctacaa
     1081 gcagatcgtc gacgcggtca tggaagcggc gaaggtcgtc cgcggcgagg caagaatcga
     1141 cgagatcggc ccctcctact cgaccgagga cgggtactac ggaaagcctc tcgtccggcc
     1201 gacggcgctt gcgaaggtgt gcggcctcgc ggactacggc gacgatcagg agctgaagat
     1261 gcccgcggag acgctccacg tcgccatggt gcagccccgc gtcgcgcacc atgcgaagat
     1321 cctggatatc gacgtttccg aagccgaaaa gatgcccgga gtggcaaaga tcatccttgc
     1381 cgccgacatc agggccgcgg ggggcacgaa catcatggcc gagggacaga tgcacgagcg
     1441 caccacagtt atggcgccga gccgcgaagt gctcaacgag aagaagatct tccggtacgg
     1501 cgacgtcgtc gccctcgccg tggcggacac gaaagaccat gcccgcgccg cagccgcgaa
     1561 ggtcaaggta acgatcgaac agctcccaga gtacctcaac ttcctcgacg ccgtcatgcc
     1621 cgacgcaatg cgcatccacg aggacactcc gaatattttc tgcatccagc cgctcctcaa
     1681 gggcgcgggg cttgaagacg cctcgaaggt cgccgggatt atcgattcct ctccctacag
     1741 cgccgaggga agtttctatt ccgccagaca acctcatctc agtatcgagg gggacagcat
     1801 acaggcctat ttcgacgagg acggctattt gacgttccag tgcaagtcgc agggcgtcta
     1861 ctcctccatc gggcgcatcg gcaactccat cggcatgccg aaggacaagc tgcgcgtggt
     1921 gatgaacccc acgggcgcca gcttcggctg gtccaccaac gccggagacc tctgcctcgc
     1981 cggagcggcg gccatggtca tgaaggcccc ggtttccctg tcgatgagct acgaggaaca
     2041 ccagcacttc tccggaaagc gctgcccctg ccactcgaac ggacgtgccg cgtgcgacga
     2101 aaacgggaag atcaccgcgg tcgagttcga tttcggcatg gaccacggcg cgtactcctg
     2161 gggcggcgac gacattgtca ccaaacagcc ccgtttcgcc ttcttccctt attacgtgcc
     2221 gcacgcggcg gggcttgccc gcatcgccaa cacgaaccac accttcggca cggcctacag
     2281 gagctacggt tccccgcagg cctacacgct gagcgaggcg ctgatggaca tgctcgccga
     2341 aaaagccggc atcgacccgt tcgagttccg ctggagaaac atcgcccgcg agggacagac
     2401 caacatcaac agctacccct tccgccagta cccgatggaa gagatgatga aaaaaatgcg
     2461 accgcactac gagcgcgccg tcgccgaggc gaaagccgcc gatacgcccg aaaagcgccg
     2521 aggcgttggc atctcctggg ggggattcaa cgtcaccgag ggcggcaccg acaaggccac
     2581 ggtcgccatc gaactgaacc cggacggcac gttcacgaag tacgacacct accaggagct
     2641 cggtcagggc ggcgacatcg gctccctgat gctcacgctg gaggcgctca aacccctcgg
     2701 cgtcaccccc gaacagatca agcaggtaca gaacgacacg aaactctgcc cggatgcggg
     2761 gatgtccgga gcgagccgca cgcacttcat gagcggcaac gccaccatca tcgccgccgg
     2821 gaagctcatc gccgccatga agaagcccga cggaacctac agaacctacg aggagatgaa
     2881 ggccgaaggg ttggagacga agtacttcgg cgcctactcg aacacttcgg tcccgggttt
     2941 gtgcagactc gacccgaaca ccggcatcgg cgatccgacg cccgccttca catactgcct
     3001 gaaccttgca gaggtcgagg tggac
//
LOCUS       DAOSWW010000241         3019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24026_length_3019_cov_3.130229, whole genome shotgun sequence.
ACCESSION   DAOSWW010000241 DAOSWW010000000
VERSION     DAOSWW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24026_length_3019_cov_3.130229"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..865)
                     /gene="speE"
                     /locus_tag="PK442_05925"
     CDS             complement(20..865)
                     /gene="speE"
                     /locus_tag="PK442_05925"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOO87222.1"
                     /translation="MEIRQKRTNELWFTEEQSVNMKLSLRVSDVLLNITSPYQDILLI
                     QTAEYGRMLVLDGAIQITERDEFCYSEMMAHVALCSHPCPKRVLIVGGGDGGVLREVL
                     RHKCVEKATLVDIDEEVINASKKFLPTINVALEDPRGYVRPMDAIEFIASSDDEFDVA
                     IVDSTDPVEFAAGLFRSPFYRGIFKALKKDGMVVAQTESPFTDASVVKGAFKEMSAVF
                     PIVRMCWGAMPTYPSGMWTYTIGSKTVDPADIRRPSPDGTKYYTEDIHKAAFVLPPFV
                     KDLLK"
     gene            complement(969..2144)
                     /locus_tag="PK442_05930"
     CDS             complement(969..2144)
                     /locus_tag="PK442_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOO87223.1"
                     /translation="MENEFQFDLERYMSQANFNRIKDFAQDKETPFLIVDLKKVEEKY
                     DELLDSLPFAKIYYAVKANPLEQVLRMLVQKGSCFDIASIYELDHILALGASPDRISY
                     GNTIKKARNIAYAYSKGVRLFATDSESDLRKLAKNAPGARVFFRILTDGSGADWPLSR
                     KFGAHPDTIFKLILLAAKLGLEPYGISFHVGSQQRDIGQWDNAISACKYLFDCAEAEG
                     ITLKMLNLGGGFPATYISPTHETSVYAAEIIRFLKEDFGENLPEIILEPGRFMAGDAG
                     VLVTEVVLISKKSEANQYSWVYLDAGKFGGLIETLDESIKYPIYSEKKGPVQETILAG
                     PTCDSMDILYENEKYALPASLEEGDRLYFFTTGAYTQTYSSVCFNGFPPLKAYVLEK"
     gene            2338..>3019
                     /locus_tag="PK442_05935"
     CDS             2338..>3019
                     /locus_tag="PK442_05935"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOO87224.1"
                     /translation="MFYGKRKVPEWAKQVAVLIVLLVLTVSAWQWYRILEHRTEQEFS
                     APVPAPSPAPTPVPAPSPEPRPDLEPGPLPTAEPPAVIQPPVAPHEARIDEAWLHVVK
                     GQYRMYFYRGETVERVYQIAIGANGGQKLRVGDHRTPVGSFSVQQIQDSRKWTYDFGD
                     GKGAIRGAYGPWFIRLKTPGWKGIGIHGTHDPDSIGRMITQGCVRLKNEDLEELRDKV
                     FVGMKVIIS"
BASE COUNT          727 a          747 c          792 g          753 t
ORIGIN      
        1 atattataga gtatacgcct tatttcagca agtctttaac aaacggcgga agcacgaaag
       61 ccgctttgtg aatgtcctcc gtatagtatt ttgttccatc gggagaaggt ctgcgtatat
      121 cggccgggtc gacagtcttc gagcctatgg tgtaggtcca cataccgctt ggataggtgg
      181 gcatcgctcc ccagcacatc cgcacgatgg ggaaaacagc gctcatttcc ttgaaagccc
      241 ccttgacaac ggatgcgtcg gtgaatggtg attctgtctg ggcgactacc ataccgtcct
      301 ttttcagcgc cttaaaaata cccctgtaga aaggggaacg gaacaacccg gcggcaaact
      361 cgaccgggtc ggtactgtca acgatggcga cgtcgaattc gtcgtcgctt gatgcgataa
      421 actcgattgc gtccatagga cgcacatagc cgcggggatc ctcaagcgcc acgttgatcg
      481 tgggcagaaa tttttttgat gcgttgatca cttcctcgtc gatatcaacg agcgtcgctt
      541 tttcgacgca tttgtggcgg agcacttcgc ggagcacacc gccgtctccg ccgccgacaa
      601 taagcactcg tttcggacat ggatgggagc agagcgcaac gtgcgccatc atctccgaat
      661 agcagaactc gtcgcgttca gtaatctgga ttgcgccgtc aagcaccagc atccttccgt
      721 actcagcggt ttgaatgagc aagatatcct gataggggct ggtgatattc aaaagaacat
      781 ccgacacgcg aagagagagt ttcatgttta cactctgttc ctcggtgaac caaagttcgt
      841 ttgtccgttt ttgacgaatt tccacgggac acgcctcctt tcacgaagaa cgccgtcaat
      901 agaacatcgg gggcgggtat cttcccgtcc ccgatggaca ttattatgtc agtataagag
      961 aacttccttt atttttcaag aacgtacgct ttaagaggag gaaagccgtt gaaacagacg
     1021 gaggagtatg tctgcgtata cgctccggtg gtgaaaaagt agaggcggtc gccctcttca
     1081 agagaggccg gaagggcgta cttttcattt tcgtaaagaa tatccatgct gtcgcatgtg
     1141 ggtcccgcga gaatggtctc ctggacgggg cctttcttct ccgagtaaat cgggtatttg
     1201 atggattcat caagcgtctc aatcaatccg ccaaacttgc cggcgtcgag atatacccag
     1261 gaatactggt tcgcttccga tttcttcgat ataaggacta cctctgtgac aaggacgcct
     1321 gcgtctcctg ccataaatct ccccggttcg aggatgattt ccggaagatt ttctccgaag
     1381 tcttccttga gaaaacggat gatttccgca gcatatacgg aagtctcatg cgtcggcgag
     1441 atgtatgtcg cagggaatcc cccgccgaga ttgagcattt ttaaagtaat tccttcagct
     1501 tcggcgcaat cgaagaggta tttgcaagcg gagattgcgt tgtcccactg accgatgtcc
     1561 cgctgctgag aaccaacatg gaaagaaatg ccgtacggtt cgagcccgag ttttgcagcc
     1621 aagaggataa gcttgaaaat agtgtcagga tgcgcgccga attttctgga gagaggccag
     1681 tcggcgccgc ttccatccgt caggatgcgg aaaaaaactc gtgctccggg ggcgtttttc
     1741 gccaactttc gcaggtcgct ctcagaatcc gtggcgaaaa gacggactcc tttggagtat
     1801 gcgtacgcga tgtttcgcgc tttttttatg gtattgccgt aactgattct gtccggcgaa
     1861 gcgccgagag caagaatgtg atccagctcg tagatggaag cgatatcaaa gcagctcccc
     1921 ttctgcacaa gcatacggag cacctgttca agggggttcg cctttacggc ataatagatc
     1981 ttcgcaaatg gcagcgaatc aagaagttca tcgtactttt cctcgacctt tttcaagtcc
     2041 acaataagaa aaggcgtttc tttgtcttgt gcaaaatcct ttatcctatt gaaattagct
     2101 tgggacatgt aacgttcgag gtcgaactga aactcgtttt ccacgtctct acctcctcct
     2161 taggggtaat ttaaaaactt atgcagtgta cgaccttcga gtcagaaatg caagggggaa
     2221 ccgaaggaaa acgagcaaaa acggtaagta ttttcctctc tttcttcttt ccgtttccat
     2281 ggtagaatca aattctgttg aaaaacttcc gcaccgaacg atgaggaggc tcctttcatg
     2341 ttttatggca aaagaaaagt acctgagtgg gcgaagcagg tagctgttct gatcgtcctg
     2401 ttggtactta ctgtttccgc gtggcagtgg taccggatac ttgagcatcg cacagaacag
     2461 gaattctccg ctcccgtccc ggcgccttct ccggcgccga ccccggttcc cgccccttca
     2521 ccggagccta ggcctgacct tgaaccgggg cctctcccca ctgccgaacc tccggcggtg
     2581 attcagccgc ctgttgcccc ccatgaggcg cggattgatg aggcgtggct ccacgttgtc
     2641 aaagggcagt atcgaatgta cttctataga ggagagactg tcgaacgcgt ataccagatt
     2701 gccattggag cgaacggcgg acagaaactg agagtggggg atcatcgtac gcctgtcggg
     2761 agtttttctg tacagcagat ccaggattca agaaaatgga cgtatgattt cggcgatgga
     2821 aaaggagcta ttcgaggcgc gtacgggccc tggttcattc gactgaaaac ccctggttgg
     2881 aagggaatcg gcattcacgg tacccacgat cctgattcta tcgggaggat gatcactcag
     2941 gggtgtgttc gcctaaagaa cgaggatctc gaagaattaa gagataaagt cttcgttgga
     3001 atgaaagtta ttatttcgg
//
LOCUS       DAOSWW010000242         3018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24048_length_3018_cov_2.618967, whole genome shotgun sequence.
ACCESSION   DAOSWW010000242 DAOSWW010000000
VERSION     DAOSWW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24048_length_3018_cov_2.618967"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PK442_05940"
     CDS             complement(<1..1074)
                     /locus_tag="PK442_05940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO87225.1"
                     /translation="MDRLIVSLMIINFAIHSYSNTFFFLAPYLSTQGYSPASAGVLVS
                     VFYAATTAVRPVGSWITERFGIRGSMIASSLLCLACAAVLHIYQGSFSFFVGLRIVMG
                     IGFSVFMVALTTYQALAVEEAKRGLAFALVAIGSIVPMFTIVPLTDFLIRNNLGRIYL
                     LLPVAASALALMLAIRLNTKQTTGFEETNSTMKWGSYRDLFRETPAWRMLCSCFFFGL
                     CDSSIVFLGSMLLEKGLVPSWFVFAMAGGALFVRTAGRNLFNRYPRTVFAGPSFLLMA
                     ACLIWLSFVDSPAGMLLSGFLYGIGVGYGYPAHLALTADLAPARLRAKASALVHFSMD
                     LSWFLLPLYMGFGSTLLGTLLAFR"
     gene            complement(1270..1440)
                     /locus_tag="PK442_05945"
     CDS             complement(1270..1440)
                     /locus_tag="PK442_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO87226.1"
                     /translation="MAKAVVDKDACVGCETCVGTCPVEAISMVDGKALVDESCIECGS
                     CVSVCPVSAITQ"
     gene            complement(1560..2585)
                     /locus_tag="PK442_05950"
     CDS             complement(1560..2585)
                     /locus_tag="PK442_05950"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOO87227.1"
                     /translation="MAEITVFGGGTWGTALAVSAAKSGHSVMLWCRRAEQARAINATS
                     HNPDYLQSIALPEAISSTSDPEEAALFSQYWILALPTQTVRGFLPALAPFCGKETEVC
                     NVAKGIEIGTGARISAIVSETLPEVRYSVLSGPSFAGEVAQGLPTALTAASRHETSAL
                     LWQSALNTQRLRIYTSSDVIGTETGGAVKNIMAIASGMASALGLGENARAALVTRGLA
                     EIMRLGEALGAHPLTLAGLAGMGDLVLTCYSTQSRNFRLGLALGRGLSLDEAKLEVGQ
                     VAEGAFTVRAVAESALALSVELPVSQGVHRLLYEGSSPQDELERLIARDPKAEYPPAM
                     LWGSGRG"
     gene            complement(2610..>3018)
                     /locus_tag="PK442_05955"
     CDS             complement(2610..>3018)
                     /locus_tag="PK442_05955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO87228.1"
                     /translation="LWGRLSDRFGRRKIAALVLPASGVALFLLTRVQGAPLIVSTLLL
                     FGIFSNTAFSPVAVAWIGDIVSKRHPGSMSAAVGFFNATIMSSAVIAPLVSGFLRDMT
                     GSLVPAILTGSLLMFFGTVMLLLTPDAARAGAE"
BASE COUNT          656 a          876 c          971 g          515 t
ORIGIN      
        1 gcgaaatgcc aaaagcgtgc cgaggagcgt cgagccgaac cccatgtaga ggggaagaag
       61 gaaccagctc agatccatgg aaaagtggac cagcgccgac gccttggccc tgagacgagc
      121 cggagcgaga tccgcggtga gcgcaagatg cgcggggtag ccgtacccga cgccgattcc
      181 gtagaggaat ccggagagca gcatcccggc aggtgaatcg acgaaggaga gccagatgag
      241 acacgctgcc atcagcagga acgaggggcc ggcgaagacg gttctcgggt agcggttgaa
      301 caggtttctc cctgctgtac ggacgaagag cgccccaccc gccatagcga agacgaacca
      361 tgacgggaca agccctttct ccagcagcat gctgccgaga aagacaatgg aggagtcgca
      421 gaggccgaag aagaaacagg agcagagcat cctccacgca ggagtctccc ggaagaggtc
      481 gcgataactc ccccacttca tggtcgagtt tgtctcttcg aagccggtcg tttgctttgt
      541 gttgaggcga atcgcgagca taagagcgag cgccgacgcg gcaaccggaa gaagcaggta
      601 gattctgccg aggttgttcc ggataagaaa gtcggtcagc gggacgatag tgaacatggg
      661 gacgatgctg ccgatggcga ccagggcaaa tgctagtccc cttttcgctt cttccacggc
      721 gagcgcctgg taggttgtaa gcgcgaccat gaagacgctg aagccgattc ccatgacgat
      781 acgaagcccg acgaagaagg agaacgaacc ctggtagatg tggagaaccg ctgcgcaggc
      841 gaggcagagc aaagacgatg cgatcatgct tcccctgatc ccgaatcgct ccgtgatcca
      901 gcttcccacg gggcggaccg cggtcgtcgc cgcgtagaac acgctcacca gcacgcctgc
      961 ggatgcgggg gagtacccct gtgtgctcag gtagggcgcc aggaagaaga acgtgttgga
     1021 gtacgagtgt atcgcgaagt tgatgatcat cagggaaacg atgagccgat ccatggaagc
     1081 ctccggaaga gagattttgc tgacgctgtt tcgcggcgga ttgtccgcct gttcgggagg
     1141 gcgtcgcgcg gaggttagtg tataccgtaa ccgcttttta ttcaatctcc tgtatcgtaa
     1201 agaaaacgcc cctccgcgga atggaggggc gtttcgaaaa aacgtgttcg taacgagatc
     1261 agagacttac tactgggtga tcgcgctcac gggacagacg gacacgcagc ttccgcactc
     1321 gatgcaggac tcgtcaacga gagccttgcc gtcgaccatc gaaatagcct cgaccgggca
     1381 ggtgcctacg caggtctcgc aacccacgca tgcatcctta tcaacaactg cctttgccat
     1441 aaatacattc ctcctcagca agggatattc tgacgctatt gaacgacttg gattatacat
     1501 cactttctta cctcgggcaa cttttgacgc aactccgtga ataaccggag atacggaggt
     1561 caccccctcc cgcttcccca gagcatcgcg ggcgggtact ccgccttcgg gtcgcgggcg
     1621 atcagccgtt ccagctcatc ctgaggagaa gagccttcgt agagaaggcg gtgcactccc
     1681 tgcgagaccg gaagttccac ggagagcgcg agcgcgcttt cagcgacggc gcggaccgtg
     1741 aacgctcctt cggcgacctg accgacctca agtttcgctt catcgaggga gaggccccgg
     1801 ccgagcgcga gcccaaggcg gaagttgcgc gactgcgtgc tgtagcaggt gaggacgaga
     1861 tcccccattc ccgcgagccc agcgagcgtc agcggatggg ctccgagcgc ttcgccgagg
     1921 cgcatgattt ccgcaagtcc gcgggttacc agcgctgcgc gcgcgttttc tcccaaacca
     1981 agggcggaag ccattccgga ggcgatcgcc atgatatttt tcaccgcgcc ccccgtttcg
     2041 gtaccgatga cgtcggacga cgtgtagatg cgcagtctct gggtattcag cgcagattgc
     2101 cagaggagcg cggacgtctc gtgcctggag gcggcagtca gtgcggtcgg aaggccttgc
     2161 gccacctccc cggcgaaact cggtcccgaa aggacggagt agcggacttc cggaagcgtc
     2221 tccgaaacga tagcgctgat tctcgctccc gtgccgatct caatcccctt tgccacgttg
     2281 cagacttcgg tctctttgcc gcagaagggg gcgagcgccg gaaggaaccc ccgcacggtt
     2341 tgcgtcggca gcgcgaggat ccagtactga gagaagaggg ccgcttcctc gggatcggat
     2401 gtcgacgaga tcgcctcggg aagagcgatc gactggagat agtccgggtt atgcgaggtc
     2461 gcgttgatcg ctcgcgcctg ttcggcgcga cggcaccaga gcatgacgga gtgcccgctc
     2521 ttggcggcgg agacggcgag cgccgtaccc caggttcctc cgccgaatac tgtgatttca
     2581 gccatgacgg gaattgctcc tttcgtgtat tactccgcgc ccgcccgggc ggcgtcggga
     2641 gtcagcagca gcattacggt accgaagaac atcagcaggg agcccgtgag gatggcgggt
     2701 accagggagc ccgtcatgtc gcgcagaaag ccggagacga gaggcgcaat caccgccgag
     2761 gacatgatcg tcgcgttgaa gaagccgacc gcggcgctca tcgagccagg gtggcgtttg
     2821 ctcacgatgt cgcctatcca tgcgactgca acgggcgaga atgccgtgtt gctgaagatg
     2881 ccgaagagca gcagcgtgga aacgatgagc ggcgctccct gaacgcgggt gagaaggaag
     2941 agcgccacgc cgctcgccgg cagcacgagc gcggcgatct ttctgcggcc gaagcggtcg
     3001 gagagcctcc cccagagc
//
LOCUS       DAOSWW010000243         3015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24076_length_3015_cov_6.125000, whole genome shotgun sequence.
ACCESSION   DAOSWW010000243 DAOSWW010000000
VERSION     DAOSWW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24076_length_3015_cov_6.125000"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK442_05960"
     CDS             <1..193
                     /locus_tag="PK442_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87229.1"
                     /translation="DPNNDTGFSSVVKMNPVALVPQVVFDEASAKVFFNPFYPNITRM
                     SKNTLPHSFLSTERLSLSQ"
     gene            complement(270..1739)
                     /locus_tag="PK442_05965"
     CDS             complement(270..1739)
                     /locus_tag="PK442_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO87230.1"
                     /translation="MPRKTAPFNFESAPEQRSLTAKPGGILLAELFHASGCAAAADAI
                     GVRSSRGYSDADHVLSSVLLNALGFDAGDDLEQLRSDGALVSAVHLLNEHKGKERRTV
                     NAFPSPSAFRAFLTDAGSTEERVRGFSGLCSVPVRCAQEMKPQEKITVDMDATIIPTE
                     RGDDVRCNYKGERSHMAFNSYVPELDMVVHSEFCPGNVSPRDDQLGLLRRTLSKLPEG
                     VKEVETRIDSAGYCMDLIKYCCGGESPYGVIRFSISVPMYKTYREEIERLSEKAWSPF
                     VDENGREETDRRWAEVNIVPTSLVSSEPYPEIRFIAVRERMKHPKLKERMDAAQMELA
                     IEEIEGEHPKTERLHLTCMDGTVWKVFLMVTNDFERNGQHALEHHWKRCGKSEEVHGI
                     LKNDLAGGHVPSADFGSCAVWWYLCVLSYNLQRLMTHRLFPEEWARAHQKRLLATIYS
                     TPAKMVRHGKQIVIRVFGPIGRLIEAAHWKVLRLGRLNI"
     gene            2415..>3015
                     /locus_tag="PK442_05970"
     CDS             2415..>3015
                     /locus_tag="PK442_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO87231.1"
                     /translation="MNTLRGRLLGIFLVLSLGGLAIIGFLAVNSSSRALVSSAEKEGL
                     ALGKGLAVETDMFFRERLNFLNLQSERNVVRTMKWETQKQGLEEAANLYGDLVDIWVI
                     SPDGSENHLTGTLAGFEGKEGLRKVLSDGKTYVSPVTVLKSAGKNVVVFGVPVESDGQ
                     RVGVLSAAFDAAGLSKFIDPVRWGTTGYADATDRNGVILA"
BASE COUNT          630 a          866 c          853 g          666 t
ORIGIN      
        1 cgacccgaat aatgatacag gattttcgag cgttgtcaag atgaatccag tcgccctcgt
       61 tccacaggtt gtttttgacg aggcttccgc aaaggttttt ttcaacccct tttatccgaa
      121 cattacccgg atgtcgaaaa acaccctccc gcattcattt ttatccacag aaaggctgag
      181 tttgtcgcaa tgaaagtaca ctccatccgc gccgcgctcc actcggcggc ggcgtcttcg
      241 ctatcgcatc acccctccct ccgtgaatat caaatattca ggcgaccgag ccgcagcacc
      301 ttccaatgcg cggcttcgat cagtctccca atagggccga acactcgtat cactatctgc
      361 tttccgtggc gcaccatctt cgcaggcgtc gagtagatcg tcgccaacaa ccgcttctga
      421 tgcgcgcgcg cccactcctc cggaaagaga cgatgcgtca taagccgctg aaggttgtac
      481 gacaggacgc acagatacca ccagaccgcg caggagccga agtcggcgct cggcacgtgt
      541 cccccggcga ggtcgttctt caggattccg tgcacctcct cggacttccc gcatcgcttc
      601 cagtgatgct cgagcgcgtg ctgtccgttc cgctcgaagt cgttggtcac catcaggaaa
      661 accttccaga ccgttccgtc catgcaggtc aggtgcagtc gctccgtctt cggatgctcc
      721 ccctcgatct cttctatcgc gagctccatc tgcgcggcgt ccattctctc cttcagcttc
      781 ggatgcttca tcctctcccg cacggcgatg aagcggattt ccggataggg ctcgctcgaa
      841 acgagcgagg tcggcacgat gttcacttcg gcccaccgcc ggtcggtctc ctccctgccg
      901 ttttcgtcca cgaaggggct ccaggccttc tcggaaagtc tttcgatctc ctcccggtac
      961 gttttgtaca tgggaacgga gatggagaag cgtatgacgc cgtacgggct ctctccgccg
     1021 cagcagtact tgatgagatc catgcagtat ccggcgctgt cgatgcgcgt ttcgacctcc
     1081 ttcaccccct cgggcagctt cgagagcgtt cttcgaagca gtccgagctg gtcgtcgcgg
     1141 ggcgatacgt ttccgggaca aaattcgctg tgaaccacca tgtccagctc gggaacgtag
     1201 ctgttgaacg ccatgtgcga tctctcgccc ttgtagttgc accggacatc gtcgccgcgc
     1261 tccgtaggga tgatggtggc gtccatgtcg acggtgatct tctcctgcgg tttcatttcc
     1321 tgcgcgcaac ggacggggac ggagcacagc ccggaaaacc cgcgcactct ctcctccgtg
     1381 cttcccgcgt cggtcagaaa ggcgcggaag gccgaagggg agggaaaggc gttcacggtg
     1441 cgtctctcct tccccttgtg ctcgttcaga agatgtacgg cggagacgag cgccccgtcg
     1501 gagcggagct gttccagatc gtcgcccgcg tcgaagccga gagcgttcag cagcaccgac
     1561 gaaagcacat ggtcggcgtc ggagtagccc ctgctgctcc gaacccctat tgcgtccgcg
     1621 gccgccgcgc agcccgatgc gtggaagagt tccgccaaaa ggattcctcc cggcttcgcg
     1681 gtaaggcttc tctgttcggg ggcggattcg aagttgaacg gagcggtctt tcgtggcata
     1741 atacggtcac ctctctggtg gagtttgttt gtggtgaacc gactttacac ccgttttcgc
     1801 aacgggacca gaggggtttt ttgtatttat tgcgtttttc tcgtatcttc cgctcctctt
     1861 ttcttttccg ccgcaagcga tatcttcgca gcaattcctc atccggcgaa cagttcgctt
     1921 tcctgatgtt tccacggtcg cagcattttt cgtaagccgt tctgcccgcc tcgaactcga
     1981 aaactcctcc cgaaaggtgg aagttgcgaa tcccgccgat tgagtcgccg caatcaatca
     2041 gacgaagatc ctatcggcaa cctgcggaac aaggggaccg gaatgaagtc tcaagaaacg
     2101 aaaaatttgg tacagaaaga ccggcggggc gatgtggggg aggagaaaag atgtcccggg
     2161 catatctgcg gccggactgt tctccggtca tctcgtgttt tccacggtac ggtccggaga
     2221 ggggtaaatg cataaaaaaa taaaaagtga agcggagtga aaataaacaa aatgagcaaa
     2281 acctcaattc tcaaaagaga acgtaagata aatatataac catattgatg atgtgggctg
     2341 tggtatgata aagtactaaa atcgaacatt ctgcagaatc gaaaatccgt agaggaaaaa
     2401 gggagaggat agagatgaac accctgagag gaaggctgct tggaattttt cttgtattga
     2461 gtcttggagg gctggcgatc atcggtttcc tggcggtgaa cagctcgagc cgggcgctgg
     2521 tatcgtcggc ggagaaggaa gggctcgccc tgggcaaggg gctggcggtt gaaacggaca
     2581 tgttcttccg ggagcggctg aatttcctca atctgcagtc tgaacggaac gtcgtcagga
     2641 ccatgaagtg ggagacccag aagcaggggc ttgaggaagc ggcgaacctc tacggcgacc
     2701 tggtcgacat ctgggtgatc tctcccgacg gttcggaaaa ccatctcacc ggaacgctgg
     2761 ccgggttcga aggaaaagag ggactgcgga aagtgctttc cgacggcaaa acctatgttt
     2821 ctcccgtgac ggttctgaag agcgccggaa agaacgtcgt ggttttcggc gttcccgtcg
     2881 aatcggacgg acagcgggtc ggtgtccttt cggccgcctt tgacgcggcg ggactcagca
     2941 aattcatcga tcccgtccgc tggggaacca cggggtacgc cgacgccacc gacaggaacg
     3001 gcgtcatcct tgccc
//
LOCUS       DAOSWW010000244         3006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24182_length_3006_cov_2.975601, whole genome shotgun sequence.
ACCESSION   DAOSWW010000244 DAOSWW010000000
VERSION     DAOSWW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24182_length_3006_cov_2.975601"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PK442_05975"
     CDS             complement(<1..246)
                     /locus_tag="PK442_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87232.1"
                     /translation="MITPNGTQELQEISTPSPGQPLCPACFSPSAGSDVPCPVCGYSA
                     SQPEEGGWNRLKPGTIIHARYIVGQPLGQGGFGITYLG"
     gene            complement(305..1144)
                     /locus_tag="PK442_05980"
     CDS             complement(305..1144)
                     /locus_tag="PK442_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOO87233.1"
                     /translation="MMIRPGNYQHIGARKEQQDAFGFSDLSDVAFVRHGGVLAIVADG
                     MGGLQEGAEASRIAVRAFLESYGGKNTDERIEDALDRSLREANNSVYAFSVERNLEGN
                     CGTTIVAVVVHESGVYWTHAGDSRLYMTDGQSLTPLTEDHIYANNLKKAVEKGLLRQE
                     DALVHPERDALTSYIGDRTIKIVGKGFLKSSPDGLPPNGFMLLLCSDGLYKTLSEKEI
                     LEVYSPDPEKWARALVKSAIAQKYRFQDNVTVLCLAAGALVPRRSEDTLNNSHAIESD
                     DVE"
     gene            complement(1157..1972)
                     /locus_tag="PK442_05985"
     CDS             complement(1157..1972)
                     /locus_tag="PK442_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOO87234.1"
                     /translation="MHYIALTGGGMLIVGCLLPWISLGTILVQQAPDTVFVAVALLSG
                     LLSTGVAIHNIVKKQNRMKYAYLAIGGICLMIGLYGLLRVRDIRTTAEFANVLQKRIT
                     AEADMPVATGIINTGLWFVAIGGLILFGTGLVNVFSQRMYAAGRGASGPRGGANGGLP
                     TFASGAQRAAILNGIAGPYAGLQIPVPLEGIIIGRDPAYSNLVIQSPAVSRRHARIMQ
                     GPSPDSCTLEDLNSTNGTFVMERNAWVRLTAPAVLTIFKRFRLGDEHTEFEIR"
     gene            complement(2012..>3006)
                     /locus_tag="PK442_05990"
     CDS             complement(2012..>3006)
                     /locus_tag="PK442_05990"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOO87235.1"
                     /translation="EAQQLLNELGYDAGPVDGKLGPLTVAAIRVFQQSRGVAWDGSVS
                     KELLGVLRTMAKKQKDAALASIGEFSRRSGGQLYRAGTSPFTSIFDSVAASLDNALAL
                     HITLEGLVPDATMKRLEITFFDGTRSLSDGIEFRLFGAQAERMPVMLPEETKESSATI
                     TDYSVWAYLLAIAAIITLPLSVWLIQKRMRTAKRESAAPDSSEKQSILPKDESSPTIV
                     LSEAAQRASLHVELTPSGARGTDTTLKAVITDRIVLGRNRGPSALSILGDATVSARHC
                     ELVFSKGRLFVNDLQSSNGTMVNGVPIQGHYPLEDGDRLTIGKTELRIRILEVK"
BASE COUNT          564 a          814 c          907 g          721 t
ORIGIN      
        1 gcccaggtag gtaatgccga agcccccttg ccccagcggt tgtccgacga tatagcgggc
       61 gtggatgatc gttcccggtt tcagacggtt ccatccgccc tcttcgggtt gagaagcgga
      121 gtacccgcag acagggcacg gaacatcaga tcccgcagat ggggagaaac acgccgggca
      181 aagaggctgt ccgggactcg gtgtggaaat ctcctgcagc tcttgtgttc cgttgggtgt
      241 gatcatgctc tccactcctg gtgtgcggct ttttatcacg gcgtcgtcat ggcacatact
      301 gctcttattc tacatcatcg gattcaatgg catgagagtt attcagggtg tcttcgctcc
      361 ggcggggtac aagtgcgcct gcggcaaggc agagtacggt cacattgtcc tggaatcgat
      421 atttttgtgc tatggcgctt ttgacgagcg cccgggccca tttctccggg tctggcgagt
      481 agacttcgag gatttccttc tcggagaggg ttttgtaaag gccgtctgag cagaggagaa
      541 gcataaagcc gttcggcggt agtccgtcgg gagagctttt gaggaaaccc ttcccgacga
      601 tcttgatcgt cctgtcgccg atgtagcttg tgagggcgtc tctctccggg tgcacgagcg
      661 cgtcttcctg tctcagcaat cctttttcaa cagccttttt tagattgttc gcatagatat
      721 ggtcttcggt gagaggcgtc aggctctgcc cgtctgtcat gtacagcctg ctgtctccag
      781 cgtgcgtcca gtaaacgccg ctttcgtgga cgacgacggc gacgatggta gtaccgcagt
      841 tcccttcgag gtttctctcg acggagaagg cgtagacgga gttgtttgcc tcccggagag
      901 agcggtcaag tgcgtcctct attctctcgt ccgtgttttt tcccccatac gactccagga
      961 aagcgcgtac cgcgatacgg gaggcttccg ctccttcctg gagtccgccc attccgtcgg
     1021 cgacaattgc aagaactcct ccgtgccgta cgaaagctac atcagaaaga tcgctgaatc
     1081 cgaaggcgtc ctgctgttct tttctggcgc cgatgtgctg gtagttccca gggcggatca
     1141 tcattctcgg cggctcctag cggatttcga attccgtgtg ttcgtctccc agtcggaagc
     1201 gcttgaagat ggtaaggacg gcgggcgcgg tcagtcgcac ccaagcgttt ctttccataa
     1261 caaaagtgcc gtttgtggag ttcaaatctt ccagcgtaca gctgtcgggg gaggggccct
     1321 gcattatgcg ggcgtgtctt cgggaaaccg cgggcgactg gatgacgaga ttggagtacg
     1381 ccggatctct tccgattata atgccttcga ggggcacggg gatctgcagg cctgcgtacg
     1441 gtccggcgat tccgttgagt atggccgccc gctgtgcgcc cgaggcgaag gtcggaaggc
     1501 cgccgttcgc tcctcctctg gggcctgatg cgccccttcc ggcagcgtac atcctctggg
     1561 agaatacgtt gacgaggcct gttccgaaga gtataagccc cccaatggcg acgaaccaga
     1621 ggccggtgtt tatgattccc gtcgcgaccg gcatatctgc ttctgcggtt atccgttttt
     1681 gcagtacatt ggcgaactct gctgttgtgc gaatatcacg tacacggagg aggccgtaga
     1741 ggccgatcat gaggcagata ccgccgatgg ccaggtaggc gtatttcatc ctgttttgct
     1801 tttttactat gttgtgaatg gcaacccccg ttgagaggag tccagagaga agtgcgaccg
     1861 ctacgaagac ggtatccggc gcctgctgaa cgagaatcgt tcccagcgaa atccagggca
     1921 ggaggcagcc gacaataagc attcctcctc ctgtcaacgc gatgtagtgc atgatgcgtc
     1981 atctcccttg cgatagtgta cgaaaaaacg gttatttcac ctcaagaatg cggatgcgaa
     2041 gctctgtctt ccctattgtc agtctgtcgc cgtcctccaa agggtagtgt ccctgtatgg
     2101 ggacgccgtt gaccatcgtt ccgttggagg actggaggtc gttcacgaag agtctgcctt
     2161 tggaaaaaac gagttcgcag tgccttgctg agacggttgc atcgccgagt atggagagcg
     2221 cggatggccc tctgtttctc ccgaggacta tcctgtcggt gatgacggcc ttgagagtcg
     2281 tgtcagttcc cctggctccg gaaggtgtca gctcaacatg cagtgaagcc ctctgcgctg
     2341 cctctgaaag gacgatggtt gggctgcttt cgtcttttgg caggatcgac tgtttctcgg
     2401 aggagtccgg ggctgctgac tctctcttcg cggtacgcat ccttttttgt atcagccaaa
     2461 cagagagagg aagagtgatg atcgcagcga ttgcgaggag gtaggcccac actgaataat
     2521 ctgtaatcgt tgcggaggat tccttagttt cttcgggaag cataactggc atccgctcgg
     2581 cctgagcgcc gaacagacgg aattcgatgc cgtccgaaag ggagcgcgtg ccgtcgaaaa
     2641 atgtaatttc gagccgtttc atagttgcgt cgggaacgag cccctcgagc gtgatatgca
     2701 gggcgagagc gttgtccagg gatgcggcga cggagtcgaa gatggacgtg aagggagagg
     2761 tccctgcgcg gtagagctgt ccgccggagc gcctggagaa ctcccctatg cttgccagcg
     2821 cggcgtcctt ctgttttttc gccatcgtcc gaagcactcc cagcagttct ttggacacgg
     2881 agccgtccca tgcgactccc ctgctctgct gaaaaacccg gatggccgcg accgtgagcg
     2941 gtcccagttt tccgtcgacg ggcccggcgt cgtaccccag ttcgttcaaa agctgctgcg
     3001 cttcgg
//
LOCUS       DAOSWW010000245         3004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24200_length_3004_cov_3.422177, whole genome shotgun sequence.
ACCESSION   DAOSWW010000245 DAOSWW010000000
VERSION     DAOSWW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24200_length_3004_cov_3.422177"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK442_05995"
     CDS             <1..573
                     /locus_tag="PK442_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOO87236.1"
                     /translation="GSCQAKALASRIGGESVFACKYDSGSIVSKTPLLKSQGNAVITK
                     LPMIGMKEHVLSKGVKKTLLEVEFDDFTLFLTHLPLGYAARSVQLGEIAERCIDAKKP
                     VILAGDGNTYRGERELRSFFRKTGFQNANPTNTPTFPSRIPALALDFVLYGPGIEMER
                     FDVPRVRYSDHLPLVCDFTVARPKKERRSQ"
     gene            570..2015
                     /gene="cls"
                     /locus_tag="PK442_06000"
     CDS             570..2015
                     /gene="cls"
                     /locus_tag="PK442_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOO87237.1"
                     /translation="MTVTMLLYSAYELSSIAIRLIMLCYIPLKHTPSTATAWLLAIYF
                     WPWLGFLLYSLFGSTSLPQERIDRHETMLRSLDEDKRMFRRIFDEEAHWLPDELVRFG
                     ALGRKLGDMCITRGNDFLLIDREEAFIRSLAADVENAQRSVHLLFYIFARDAVTEPLF
                     RALEGAAARGVACRVLVDALGAKPFLKRDAERLRAAGIRVEKALPPRLLRRTPTTARY
                     DLRNHRKIAVIDGRVGYTGSHNVTEPSYGGKAKGLLWRDLTLRLVGPVVLQLQGVFME
                     DWFVETREMLLSDEAFPAPEYAGEAVVQTVPSGPSYPWQNYQRLVVSALHGAKKRVVI
                     TTPYLIPDDELLHALETAVLGGVRIQLVIPEKSDQFLVGNAAKAYFDALLEAGVEICL
                     FQDGILHSKTMTVDHDLAFLGTSNFDIRSFALNFELNLVLYGRGETEAIRKAQDVYIA
                     SSRRLTAEEWMERPRAFRVLHGVTKLFSPLL"
     gene            2118..2789
                     /locus_tag="PK442_06005"
     CDS             2118..2789
                     /locus_tag="PK442_06005"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HOO87238.1"
                     /translation="MKRIHLLRAAVIAALLFAFAGNAAGESAEYSDPARWLSLPSYTL
                     MDTDIFYLYPTACRNEEAAIRGVDDPCMAEGALELFERELPVFEKLGDVYAPYYSQAS
                     AYRSSLPRERRRELVNGAPRRDVFAAFDYYMEHYNLGRPFILVGSCQGAEVLIQLLSE
                     YISKDSPIYGRMRAAYLSDCDVPEDFLLSNPHLRLARTPDGDGFVISSAGDVSAVPGS
                     EPQKQ"
BASE COUNT          540 a          950 c          921 g          593 t
ORIGIN      
        1 ggctcctgtc aggcgaaggc cttggcctcc cggatcgggg gcgagtcggt tttcgcatgc
       61 aagtacgact ccggctccat cgtttcgaag acgccgctgc tgaaatcgca ggggaacgcc
      121 gtcatcacga agctccccat gatcgggatg aaggagcatg tgctgagcaa gggtgtcaag
      181 aagacgctgc tcgaagtcga gttcgacgat ttcacgctct tcctgacgca tcttcccttg
      241 gggtacgccg cacggtccgt gcagctcggc gagatcgcgg agcgctgcat cgacgcgaag
      301 aaaccggtta tcctggccgg cgacggcaat acctacagag gtgagcggga actgcgctcg
      361 ttcttccgga agaccggttt tcaaaacgcc aatccgacga acacgccgac attccccagc
      421 aggattccgg cgctcgcgct cgactttgtt ctctacggtc cggggatcga gatggagcgt
      481 ttcgacgtgc cgcgggtccg ttattcggac catctgccgc tggtgtgcga cttcacggtc
      541 gcccgcccta aaaaggagcg acgctctcaa tgaccgtcac gatgctcttg tattcggcct
      601 atgaactttc ttcgatcgcc atccgcctca tcatgctctg ctacatcccg ctgaagcaca
      661 ccccctccac ggcgaccgca tggctgctcg ccatctactt ctggccgtgg ctgggcttcc
      721 tgctgtactc tctcttcggc tcgaccagcc tgccccaaga gcgaatcgac cgccatgaaa
      781 cgatgcttcg cagcctcgac gaggacaaac ggatgttccg ccgcatcttc gacgaagaag
      841 cccactggct tccggacgaa cttgtgcgct tcggcgcgct cggcagaaag ctcggcgaca
      901 tgtgcatcac ccgggggaac gacttcctcc ttatcgaccg ggaagaggcc ttcatccggt
      961 cgcttgccgc cgatgtggag aacgcgcagc gctccgtcca cctgcttttc tacatttttg
     1021 cccgcgatgc ggtaaccgag cctctcttcc gcgcgcttga aggcgcggcg gcgcggggcg
     1081 ttgcgtgccg cgtactcgtc gacgcgctcg gggccaagcc attcctgaag cgcgacgccg
     1141 aacgccttcg agcagcggga atcagggtgg agaaggcgct gcctcctcgg ctgcttcgcc
     1201 ggacgccgac caccgcccgc tacgacctcc gcaaccaccg gaagatcgcc gtgatcgacg
     1261 gccgcgtagg gtacaccggg tcgcacaacg tcacggagcc ttcctacggc ggaaaggcga
     1321 aggggctgct ctggcgcgac cttacgctcc ggctcgtcgg ccccgtggtg ttgcagcttc
     1381 agggggtctt catggaggac tggttcgtcg agacgcggga gatgctcctc tccgacgagg
     1441 cgtttccggc tcccgaatac gcgggcgagg ctgtcgttca gacggttccg agcgggccgt
     1501 cctatccgtg gcagaactac cagcggcttg tcgtctcggc gctccacggc gcgaagaagc
     1561 gcgtggtcat cacgacgccg tacctcattc ccgacgacga actgcttcac gcgctggaga
     1621 cagcggtgct cggcggcgtg cgtatccagc tcgtcatccc cgagaaaagc gaccagttcc
     1681 tcgtggggaa cgccgcgaag gcctactttg acgcgctgct tgaggccgga gtggagatct
     1741 gcctcttcca ggacggcatc ctgcactcga aaactatgac ggtggaccac gacctcgcct
     1801 tcctcgggac gagcaacttc gatatccgct cctttgcgct gaatttcgag ttgaaccttg
     1861 tgctctacgg aagaggcgag acggaggcta tccggaaggc gcaggacgtc tatatcgcct
     1921 cttcgaggcg tctgaccgcg gaggagtgga tggagcgtcc ccgcgcgttc cgcgtgctcc
     1981 acggagtgac gaagctcttc agtccgctgc tgtaaaccga gtcgtcgtga acctgttgac
     2041 gaaatgagga aatgacatat acttcgccgt ggatcagcgg atttaaagaa agaaacaagg
     2101 aggaacaacg taccgtgatg aagcgaatac atctcttgcg ggcagccgtt attgccgctc
     2161 tgctgtttgc ttttgcaggg aatgccgccg gcgaaagcgc cgagtattcg gatcctgcgc
     2221 gctggctctc gttgccgagc tatacattga tggataccga tattttctat ctttacccta
     2281 ctgcatgcag gaatgaagaa gctgcgattc gcggcgtcga cgatccgtgc atggcggaag
     2341 gggcgctgga gctcttcgag cgggagctgc cggttttcga aaagctgggg gatgtgtacg
     2401 ccccctacta cagccaggcg tcggcctacc gcagctcgct gccccgggag aggcggcggg
     2461 aacttgtgaa cggcgctccc cggagggacg tcttcgcggc gttcgactac tatatggagc
     2521 actacaacct cgggcggccg ttcatcctcg tcgggagctg ccagggcgcg gaagtactga
     2581 ttcagctgct ttccgaatac ataagcaagg attcgccgat ttacggaagg atgcgcgccg
     2641 catacctcag cgactgcgac gtccccgagg acttcctgct gtcgaacccg caccttcggc
     2701 ttgcacggac gcctgacggg gatggctttg tcatctcgtc cgcaggggat gtctccgccg
     2761 ttccggggag tgagccgcag aaacagtaac gcggagcacc cctctttggc atacaacgcc
     2821 gcgcccggag tttccttcag ggcgcggcgt ttacgttccc ggcagctcga tctcgacggc
     2881 gagcccgccc tccggacggt ttcgcgcgct gataacgccg ccgtgcagcg tcgccgccct
     2941 gtgcgcgatg gcgagcccaa gccccgtgcc ttcgcttgcg gatatcctct cgcgggcgcg
     3001 ctcg
//
LOCUS       DAOSWW010000246         3001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24234_length_3001_cov_3.811270, whole genome shotgun sequence.
ACCESSION   DAOSWW010000246 DAOSWW010000000
VERSION     DAOSWW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24234_length_3001_cov_3.811270"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PK442_06010"
     CDS             complement(<1..654)
                     /locus_tag="PK442_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87239.1"
                     /translation="MDIQSKSVTLEQVSKIFKDPQSGKDVHAVDGADFVIAPGELVTL
                     LGPSGCGKTTTLRMIAGFELPTKGKIRIGAEDITFLPPNKRDTAMVFQSYGLFPHMNV
                     FDNVAYGLRLRKIPENEIQEKVMRFLGMVGLDTLAKRPPSRLSGGQQQRVALARSLIV
                     EPAVLLLDEPLSNLDALLREQMRVEIRRIQKSLGITAAYVTHDRVEAMSLSDRIIVMN
                     "
     gene            complement(667..2802)
                     /locus_tag="PK442_06015"
     CDS             complement(667..2802)
                     /locus_tag="PK442_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO87240.1"
                     /translation="MNNALTGSFQSIAENHYRKSAVAAMGSVPSNDDAVSGWLRRIAP
                     SSGDFALIFLKGFPEDEEGMKVFTGAPELEAFFEMHRESDAFRKGIESSMYDEIFQPA
                     ERLQFAGEERRILFAPAHDGETGDVTGTAVFLFEPAGFDKFLVLMRGLFILALVIFTA
                     VSAVASYSRDPVLGYVILGLFAIVGVFVAYPLFEAVRLSFIQDGKFTLDTWKSILTAR
                     QYLTALQGSITLGVFTATASTIVGFLFAFVISRTGIGGKKFFSAMATLPIISPPFSLT
                     LSIILLFGNNGLITKRILALEGFSIYGLGGLTLVQTMGMFPIAFLTMIGVLEAIDSTL
                     EDAAMNLRATKWETFRTVTFPLAVPGLLSAWLLVFTNSLADFANPLILAGSFRVLSVE
                     SYMEVTGMNRLGHGAALSLLLLLPTLSAFLVQRYWVSRKSYVTVTGKPSGRMTDLVSP
                     GLKKVLVALMCLIVLFLLSLYGTIIAGCFVKNWGIDYSFSLENIREAFERGWSVLLDT
                     LTLAGAATPIAGLLAMITALVLARKKFPGKRLLDMLILTPFALPGTLVGISYILAFNK
                     APLILVGTGAIIVISYVIRELPVGVEAGIASLKQIDPAIEEAAADLGADTPTVFRTIV
                     LPLIRPAFISSLSYTFVRSMTAVSAVIFLISAKWYHLTVLIYNFSENLRFGLASVLAS
                     TLIVIVLLTFGLLRLLVRKNENLAKTMSL"
BASE COUNT          613 a          818 c         1023 g          547 t
ORIGIN      
        1 gttcatgacg atgatcctgt cggagaggct catcgcctcc acccggtcgt gggtaacgta
       61 tgcggcggtg atcccgagcg acttctggat acggcggatc tccacgcgca tctgctcgcg
      121 gagcagcgcg tcgagattgg agagcggttc gtcgagcagc aggacggcgg gctccacgat
      181 gagggagcgc gcaagggcca cgcgctgctg ctggcctccg gagagacggg acggggggcg
      241 cttggcgagc gtgtcgagcc ccaccatgcc gaggaagcgc atgaccttct cctggatttc
      301 gttctccggg attttccgaa ggcgcagccc gtaggccacg ttgtcgaaga cgttcatgtg
      361 cgggaagaga ccgtagctct ggaagaccat cgccgtatcc cgtttgttgg gagggaggaa
      421 ggtgatgtct tcggcgccga tgcggatctt ccccttcgtc ggcagttcga accccgcgat
      481 catgcggagc gtcgtcgtct tgccgcaccc ggacggaccg agcagcgtga ccagctctcc
      541 cggtgcgatg acgaagtcgg cgccgtcgac ggcatgcacg tcctttcccg actgcgggtc
      601 cttgaatatc ttgcttacct gttcgagtgt gactgatttc gattggatgt ccataactat
      661 cccaccttag agactcatgg tcttcgcgag gttttcgttc ttccgaacca gaagacggag
      721 gaggccgaac gtcaggagca cgatgacgat gagcgtgctc gccaaaacgc tggcaaggcc
      781 gaagcgcagg ttctcggaga agttgtagat cagcacggtg aggtggtacc acttggcgga
      841 gataaggaag atgaccgcgc tgaccgccgt catggagcgg acgaaggtgt acgacagact
      901 cgagatgaag gcgggcctga tgagcgggag gacgatggtg cggaagacag tgggcgtgtc
      961 cgccccgagg tccgcagcgg cctcctcgat cgccgggtcg atctgtttca gcgaggcgat
     1021 gcccgcttcg acccccaccg gcagctctcg tatgacgtag ctgatgacga tgatggcgcc
     1081 cgtcccgacg aggatgagcg gggccttgtt gaaggcgagg atgtagctga taccgaccag
     1141 cgttccggga agggcgaagg gcgtcaggat gagcatatcg agcagccgct tgccggggaa
     1201 cttctttctg gcgagcacga gagccgtgat catggcgagc aggcctgcga tgggtgtggc
     1261 ggccccggcg agggtcagcg tgtccagcag gacgctccat ccgcgttcga acgcctcccg
     1321 gatgttctca aggctgaagg agtagtcgat gccccagttc ttcacgaagc accctgcgat
     1381 gatggtgccg tagaggctca gaaggaagag gacgatgagg cacatcagcg cgacgaggac
     1441 ttttttcagc cccggggaga caagatcggt catcctcccc gacggcttgc ccgtcacggt
     1501 gacgtacgac ttcctgctca cccagtagcg ctggacgagg aacgccgaga gcgtcggcag
     1561 cagaaggagc agcgagagcg ccgcgccgtg gccgagccgg ttcatcccgg tgacctccat
     1621 gtacgattcc actgaaagga cgcggaagct ccccgcgagg atcagcgggt tggcgaagtc
     1681 ggcaagcgaa ttcgtgaaga cgagcagcca cgcgctcaat agccccggga ccgcgagcgg
     1741 gaaggtgacg gttctgaagg tttcccactt ggtggcgcgg aggttcatcg ccgcgtcttc
     1801 cagggtggag tcgatggctt ccagaacgcc gatcatggtg agaaaggcga tggggaacat
     1861 ccccatggtc tgtaccagcg tcagtccgcc gagcccgtag atgctgaatc cttcgagcgc
     1921 gagtatcctc tttgtgataa ggccgttgtt gccgaagaga aggatgatcg agagcgtcag
     1981 ggagaagggg ggcgagatga tcgggagcgt cgccattgcg gagaagaact tctttccgcc
     2041 gatcccggtc ctggagatga cgaaggcgaa gaggaagccg acgatggtgg aagccgtggc
     2101 ggtgaagacg ccgagtgtga tgcttccttg cagggcggtg aggtactgcc gtgcggtcag
     2161 gatggacttc cacgtgtcga gcgtgaactt cccgtcctgg atgaaggaga gtcggacggc
     2221 ctcgaagagc gggtaggcga cgaagacgcc gacgatggcg aagaggccga ggatgacgta
     2281 cccgagaacg gggtcgcggc tgtaacttgc cacggcggag acagcggtga agatgacgag
     2341 ggccagaatg aagagccctc tcatgaggac gagaaacttg tcgaagcctg cgggttcgaa
     2401 aagaaagacc gctgtgccgg tcacatcgcc cgtctcgccg tcgtgggccg gggcgaagag
     2461 aatgcgccgc tcttcgccgg cgaactggag gcgctcggcg ggctggaaga tctcgtcgta
     2521 catgctgctt tcgatcccct tgcggaaggc gtcgctttcc cggtgcattt caaagaatgc
     2581 ttcgagttcg ggagcgccgg tgaagacctt cattccctcc tcgtcctcgg ggaagccctt
     2641 gaggaagatc agggcgaaat cgcccgaaga gggagcaata cggcggagcc aaccggaaac
     2701 ggcgtcgtcg tttgaaggca ccgaccccat cgcggcgacg gccgatttac ggtagtggtt
     2761 ctccgcgatc gactggaagg agcctgtaag cgcgttgttc atccaaaaaa gtagcgttgc
     2821 gaaaagaaga acggacagaa acaacgagag aaatcttttc gaaggaatgt tcctctcctc
     2881 ctttccattg cgtttgaaga aaaggcgaag gagccccctt gggagcccct tcgtattttc
     2941 acagtgcggc ggatgctacc tggcggagcc gatctccttg ttccagcgtt ccagcagacg
     3001 t
//
LOCUS       DAOSWW010000247         2994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24326_length_2994_cov_3.880572, whole genome shotgun sequence.
ACCESSION   DAOSWW010000247 DAOSWW010000000
VERSION     DAOSWW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24326_length_2994_cov_3.880572"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..1556
                     /locus_tag="PK442_06020"
     CDS             321..1556
                     /locus_tag="PK442_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOO87241.1"
                     /translation="MDAAGKLSILAEAAKYDVSCSSSGNGGRRGAVGNTSLGGICHSW
                     SDDGRCISLLKILFSNDCAFDCAYCVNRRSNDVPRASFTPRELADLVIGFYRRNYIEG
                     LFLSSAVVGSPDDTMIQLIRTAKILRDEYRFGGYIHAKAIPGASPRLVTALGFLADRM
                     SVNIELPSEVSLRALAPQKTKESIFRPMSLIGDSIAERKEKRLVSTRSLAPFVPAGQS
                     TQLIVGASPETDLSILSLAGGLYHRFGLKRVYYSAYVPVSANPLLPAITTTPPLLREH
                     RIYQADWLLRFYGFRPDEVLSPEQPNLDESLDPKTSWALRHLDLFPLDINRASYEELL
                     RTPGIGVLSAKRILTARKGRRLDEDDLKKLGVVMKRARYFLACKGFSLPSLDARALRR
                     LLTPPPPPAWKTRQLSLEF"
     gene            1560..2303
                     /locus_tag="PK442_06025"
     CDS             1560..2303
                     /locus_tag="PK442_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HOO87242.1"
                     /translation="MATWVYDGSFNGFLCLLFESARAGEPPEAIEHAGSGQPFLWPPK
                     EARTDEQLAKRTGDVLRRRLSAPVFTRGYYAFLSALKEREMSVYTYYNLAWTLGKSVD
                     RLLTDPRVLPVHEASRAVLRERHRFLGLLRFTEIGGVLYAPFEPEADLLQLLAGHFST
                     RLGNEKWIIHDLGRGKAAIYAPRLWRIADFSLPEGIAPSRREEAFRDLWKEYFHSIAV
                     RSRENPRLQRQFMPKKYWKHLPEKEPPAE"
     gene            2390..>2994
                     /locus_tag="PK442_06030"
     CDS             2390..>2994
                     /locus_tag="PK442_06030"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOO87243.1"
                     /translation="MAKKFLDKDFLLHSKAAQKLYHDYAAPMPIFDYHCHIPAEEIAG
                     DTQFASLAEAWLGGDHYKWRAMRACGVDERFVTGDASPEEKFAAWAKVVPQTIGNPLY
                     HWTHLELQRIFGVETLLNADTAAEIHDHCSKMLRKKEFSVRGLIRKFNVAALCTTDDP
                     ADSLEHHEAIKKSGFETLVAPAFRPDRALDAHLPDQFNPWL"
BASE COUNT          623 a          993 c          807 g          571 t
ORIGIN      
        1 cacgtaccag tgaatagatt ccatgcgtcc ggtacgcttc ggagaatcgg cgttcaacga
       61 ggaaaaagcc ttcaaagcgt tcatcctccc agcaaccaga tacgtgcggc acttcttcag
      121 ctcggactcc ggtagccatt atagcaaact tttcagcgca gacgaggcat ggatacaaaa
      181 caccctcctt tttttcgtac gcacacacgc aatgctgttc aacgatctgc ccacttcccc
      241 agttttccgg agggcttttc atttccgcga aataatgata acatttggtt agcataacct
      301 tgaggaaggg ggcgaaaaga atggatgccg ccggtaagct gtcaattctt gccgaagccg
      361 cgaaatacga cgtttcctgc tcctccagcg gaaacggagg acgaagagga gcggtcggca
      421 acacatcgct tggcggcatc tgccacagct ggtcggatga cggacggtgc atctcgctgc
      481 tcaagatcct cttctccaac gactgcgcct tcgactgcgc ctactgcgtc aaccgaagaa
      541 gcaacgacgt gcctcgggca tcattcaccc cgcgtgagct cgccgacctc gtcatcggat
      601 tctaccgcag gaactacatc gaggggctct tcctgagttc tgcggtcgtc ggttcccccg
      661 acgacaccat gattcagctc atccgcacgg cgaagatcct ccgcgatgag taccggttcg
      721 gaggctacat ccacgcgaag gccattccgg gtgcgtctcc caggttggtc accgcgctcg
      781 gtttccttgc cgacaggatg agcgtcaaca tcgaactccc ttcggaggtc agcctgcggg
      841 ccctcgcgcc ccaaaagacg aaggagtcca tctttcgtcc gatgagcctc atcggcgact
      901 ccatcgccga acggaaggag aaacgcctcg tctccacacg ttcgctcgcg ccgttcgtcc
      961 ctgcaggaca gagcacgcag ctcatcgtgg gcgcctctcc ggagaccgac ctttccatcc
     1021 tcagcctggc gggagggctc tatcaccgtt tcggtcttaa gcgcgtctac tactccgcat
     1081 acgtccccgt ctcggcgaac ccgctcctcc ccgcgatcac cacaacgccg ccgctcctgc
     1141 gcgaacacag aatctaccag gccgactggc tgctccgctt ctacggattc cgtccggacg
     1201 aagtcctctc gccggagcag ccgaatcttg acgagtcgct cgacccgaag acgtcatggg
     1261 cgctccgcca tctcgacctc tttccgctgg acataaaccg cgcctcctac gaggagctgc
     1321 tccgaactcc gggcatcggc gtcctctcgg caaaacgcat tctgacggca cggaagggac
     1381 gacgcctcga cgaggacgac ctgaagaagc tcggagtggt gatgaagcgg gcgcgctact
     1441 tcctcgcctg caagggattc tccctcccct cccttgatgc gcgggcgctg cgccgtcttc
     1501 tcactcctcc tccgcccccc gcgtggaaga cccgccagct ctcgctggag ttctgacgag
     1561 tggccacctg ggtctacgac ggcagcttca acggattcct ctgtctgctc ttcgaaagtg
     1621 cgcgcgcagg agaaccgccc gaggcgatcg agcacgcggg cagcggacag ccgtttctct
     1681 ggccgcccaa agaagcccgg acagatgagc agcttgcgaa gcgaaccggc gacgttctgc
     1741 gccgtcgtct ttcggcccct gtattcaccc gggggtacta cgcctttctc agcgcgctca
     1801 aagaacgcga aatgtcggta tatacctatt acaatctcgc gtggacgctc ggcaagagcg
     1861 tggacagact gctgaccgat ccgcgcgtcc tgccggtcca cgaagcgagc cgtgccgtcc
     1921 tgagagagcg ccaccgtttc ctggggcttc tccgcttcac cgagatcggc ggcgtgctct
     1981 acgctccgtt cgagcccgag gccgatctcc ttcagctgct cgccgggcac ttctcaacga
     2041 ggttaggaaa cgagaagtgg atcatccacg acctcgggag gggaaaagca gcgatctacg
     2101 ctccgcgcct ctggcgcatc gccgacttca gcctccccga aggaatcgcc ccgtcccgcc
     2161 gggaggaagc attccgcgac ctgtggaaag agtatttcca ctccatcgcc gtccggtcga
     2221 gggagaaccc gagactccag agacagttca tgccgaagaa atactggaaa caccttccgg
     2281 aaaaggagcc tcccgccgaa tagttcctac gatttccccg tgcaaaaatt cttatcatag
     2341 agtaaaatac attttcagag tatgaaacca tttgcaagga ggagacgcta tggcgaaaaa
     2401 gtttctggat aaggatttcc ttctgcattc caaggcggcg caaaagctct accacgacta
     2461 cgccgccccg atgccgatat tcgactacca ctgccatatt cccgcggagg aaattgcggg
     2521 cgatacacag ttcgcctccc ttgccgaggc gtggctggga ggagatcact acaagtggcg
     2581 cgctatgagg gcgtgcggcg tcgacgagcg cttcgtcacc ggcgacgcct cccccgagga
     2641 gaagtttgcc gcgtgggcga aggtagttcc tcagaccatc ggcaacccgc tctaccactg
     2701 gacgcacctc gaactgcagc gaatcttcgg cgtcgagacg ctgttgaacg cggacacggc
     2761 cgccgaaata cacgaccact gttcaaagat gctccgaaag aaggagttct cggtccgcgg
     2821 gttgatccga aaattcaacg tcgccgccct gtgtaccacc gacgaccccg ccgactcgct
     2881 tgaacaccac gaggccatca aaaaaagcgg cttcgagacg ctcgtcgcgc ccgcttttcg
     2941 ccccgaccgc gccctcgacg cccacctccc ggaccagttc aatccgtggc tcga
//
LOCUS       DAOSWW010000248         2992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24360_length_2992_cov_4.288049, whole genome shotgun sequence.
ACCESSION   DAOSWW010000248 DAOSWW010000000
VERSION     DAOSWW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24360_length_2992_cov_4.288049"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PK442_06035"
     CDS             complement(<1..273)
                     /locus_tag="PK442_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87244.1"
                     /translation="MRKLKEMFMMNIRQYAMMLALLFIVLLFQYLTDGVLLRPLNLTN
                     IIQQNGYILILAIGMLLCILTGNIDLSVGSVAAFVGAVGGTLMTVWK"
     gene            complement(273..1817)
                     /gene="gguA"
                     /locus_tag="PK442_06040"
     CDS             complement(273..1817)
                     /gene="gguA"
                     /locus_tag="PK442_06040"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO87245.1"
                     /translation="MAKTLLRMKSITKTFPGVRALNDVCFDVEEGEIHALVGENGAGK
                     STLMNILSGVYPFGSYEGEIVFDGETCVFNTIKDSEKIGIVIIHQELALVPYLSIAEN
                     VFLGNERARRGVIDWSETYARTDDLLKQIRLHENPATITKDLGVGKQQLVEIAKAISK
                     DVRLLILDEPTAALNDEDSNNLLNLLLDLKKKGITSIIISHKLNEVEKIADRITVIRD
                     GAVIETLDNATKSIREDRIIKGMVGREIVDRFPKRPPHARGDLALEVRDWTVGDPLFF
                     DRKKIKGVSFNVHKGEVVGLVGLMGAGRTELAMSLFGRSYGSDFSGCLFLGGQSATLH
                     SPKEAIARGMAYVTEDRKSAGLILIDDIRHNISLASLGKLSDKRGVLDENKEITVAED
                     LRKKMSIKTPSVFQKVGNLSGGNQQKTILARWLFANPDVLLLDEPTRGIDVGAKYEIY
                     CIINELAAMGKAIVLISSEMPEVLGMSDRIYVMNEGRFVGELDRESASQEAIMKCIMQ
                     SNREVA"
     gene            complement(1906..2958)
                     /gene="chvE"
                     /locus_tag="PK442_06045"
     CDS             complement(1906..2958)
                     /gene="chvE"
                     /locus_tag="PK442_06045"
                     /inference="COORDINATES: protein motif:HMM:NF040907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO87246.1"
                     /translation="MKKMLLALFALVLLFSATAAFAADVLVGVAMPTQSLQRWNEDGA
                     NMKKILEGKGYKVDLQYAENNVSVQISQLENMITKGVKVMIIASIDGSALSDVLAKAA
                     EAKIPVIAYDRLIRQSPNVDYYATFDNFKVGVIQGKFIEEKLGLKDGKGPFNIEFFGG
                     SPDDNNAFFFNGGAMSILQPYLDSGKLVCHSGQTKMEQIGILAWKSEDAQARMDNLIS
                     RSYTDKKLDAVLSPNDSLAYGISASLENNGYIPGKDWPIITGQDCDRANVKNMIAGKQ
                     SMSVFKDTRALATKVAEMVDDILKGKQPEVNDTKTYDNGMKVVPSYLLEPVFADVNNY
                     KELLLDSGYYKEEDLK"
BASE COUNT          554 a          819 c          907 g          712 t
ORIGIN      
        1 cttccagacc gtcatcaggg ttccgccgac ggccccgacg aaagcggcca ccgagccgac
       61 cgagaggtcg atgtttccgg tgagaatgca gagcagcatg ccgatcgcca ggatgagaat
      121 gtagccgttc tgctgaatga tgttcgttaa gttcagcgga cggaggagca cgccgtccgt
      181 gaggtactgg aaaagaagca cgatgaagag gagcgcgagc atcatcgcgt attgtcggat
      241 gttcatcatg aacatctctt tcagcttgcg catcaggcca cctcccgatt gctctgcata
      301 atgcacttca tgatcgcttc ctgcgaggcg ctctcacgat ccaattcgcc gacgaagcgc
      361 ccctcgttca ttacgtatat ccggtcgctc atgccgagca cctccggcat ctccgacgag
      421 atcaggacga tcgccttgcc cattgccgcc agttcgttga tgatgcagta tatttcgtac
      481 ttcgccccca cgtcgatgcc gcgcgtcggt tcgtcgagca gcagcacgtc ggggtttgcg
      541 aagagccagc gggccagaat ggttttctgc tggtttccgc cggagagatt gcctactttc
      601 tgaaagacgc tcggggtctt gatggacatc ttcttccgca ggtcttcagc caccgtgatc
      661 tctttgttct cgtcgagaac gccccgcttg tcgctcaact ttccgaggct ggcgagggag
      721 atgttgtggc ggatgtcgtc gatgagaatc agtcctgcgg atttccggtc ctcggtgacg
      781 tacgccatgc cgcgggcgat cgcctccttg ggggagtgaa gcgttgccga ttggccgccc
      841 agaaagagac agccggaaaa atcgctcccg taggagcgtc cgaagaggct catggcgagt
      901 tctgtccgcc cggcgcccat caggccgacg agtcctacga cctcgccctt atggacgttg
      961 aagctgacgc ctttgatctt tttgcggtcg aagaagagag ggtcgccgac agtccagtca
     1021 cggacttcaa gagcgaggtc gcctctcgcg tgaggagggc gtttcgggaa tcggtcgacg
     1081 atctcccgcc ctaccatccc cttgatgatg cggtcctccc tgatgctttt tgtcgcgtta
     1141 tccagcgtct cgatgacggc gccgtctcgg atcaccgtga tgcggtctgc gatcttctcc
     1201 acttcgttga gcttgtgcga gatgatgatg gacgtaatgc ccttcttctt caaatccaga
     1261 agcaggttca gcagattgtt gctgtcctcg tcgttgaggg ctgcggtcgg ttcgtcgaga
     1321 atgagcaggc gcacgtcttt tgagatggct tttgcaatct ccacgagctg ctgcttcccc
     1381 acgccgagat cttttgtgat agtcgccggg ttttcgtgca gtcgtatctg tttgagaaga
     1441 tcgtcggtgc gcgcgtaggt ctcgctccag tcgatcacgc cccgccgggc ccgttcattg
     1501 ccgagaaaga cgttctcggc aatcgagagg taggggacga gcgcgagctc ctggtggata
     1561 atgacgatgc cgatcttttc gctgtctttt atcgtattga agacgcaggt ctccccgtcg
     1621 aagacgatct ccccttcgta ggacccgaag gggtagacgc cggagagaat gttcatcagg
     1681 gtggattttc ccgcgccgtt ctcgccgacg agcgcgtgaa tctccccctc ttcgacgtcg
     1741 aaacagacgt cgttcagagc gcggactccg ggaaaggtct tcgtgatgct cttcatccgc
     1801 aggagtgttt tggccatgct ctcatctcct gttcctgatt caagggggac ggagcgcgat
     1861 ggccccgtcc cctccgtcac gatatgctgc gtgagtgttc ccggattact tcagatcctc
     1921 ttccttgtag tagccggaat cgaggagcag ttccttgtag ttgttgacgt cggcgaagac
     1981 gggctcaagc aggtaggaag gaacgacctt catgccgttg tcgtaggtct tcgtgtcgtt
     2041 cacttcgggc tgtttgccct taagaatgtc gtcgaccatc tcggcgacct tcgtagcaag
     2101 cgcgcgggtg tccttgaaga ccgacatcga ctgcttcccg gcgatcatgt tcttcacgtt
     2161 tgcgcggtcg cagtcctggc cggtgatgat gggccagtct ttgccgggga tgtagccgtt
     2221 gttctcaagg gaggcggaga tgccgtaggc gagcgagtcg ttgggggaga gaacggcgtc
     2281 gagcttcttg tcggtgtacg acctggagat gaggttgtcc atacgagcct gagcgtcctc
     2341 ggatttccat gcgagaatgc cgatctgctc catcttcgtc tggccggagt ggcagacaag
     2401 cttgccgctg tcgaggtagg gctgcaggat gctcattgcg ccgccgttga agaagaacgc
     2461 gttgttgtcg tcgggggaac cgccgaagaa ctcgatgttg aagggaccct tgccgtcttt
     2521 cagtccaagc ttctcttcga tgaacttgcc ctggatgacg ccgaccttga agttgtcgaa
     2581 cgtggcgtag taatccacgt tgggggactg gcggataagg cggtcgtagg cgatgacggg
     2641 gatcttcgct tccgcggcct tggcgaggac gtcggagaga gccgaaccgt cgatggaggc
     2701 gatgatcatg accttgacgc ctttggtaat catgttctcg agctgggaga tctgaacgct
     2761 gacgttgttc tcggcgtact gcagatcgac tttatagcct tttccctcga gaattttctt
     2821 catgttcgcg ccgtcttcat tccagcgctg gagcgactgc gtgggcatgg cgacgcccac
     2881 aagaacgtcc gctgcgaaag cggctgtggc ggagaagaga aggacaagcg cgaaaagggc
     2941 gagcagcatt ttcttcataa aaaccacact cctttgaaga tggtaacgaa gg
//
LOCUS       DAOSWW010000249         2991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24372_length_2991_cov_4.194823, whole genome shotgun sequence.
ACCESSION   DAOSWW010000249 DAOSWW010000000
VERSION     DAOSWW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24372_length_2991_cov_4.194823"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PK442_06050"
     CDS             complement(<1..501)
                     /locus_tag="PK442_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOO87247.1"
                     /translation="MDAVGVNKCIIITGMSGAGKSTALKILEDQGMFAIDNIPPVLLS
                     QLLFLLEKHRAAVRWGVAAVVDIRGDSLLDDFPAALALLRKQTRHVHILFFDAKDEIL
                     LRRFETTRRRHPLEPDGSFLDGIAKERERLQPILDYADTIIDTSSLSLSAIRKELISR
                     FFEDAAA"
     gene            complement(569..1303)
                     /locus_tag="PK442_06055"
     CDS             complement(569..1303)
                     /locus_tag="PK442_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOO87248.1"
                     /translation="MNGTEKPFFLLSPGEREKLPEKSVGFVLSKDALKFSYFLSSGED
                     VLFRILEGNPLPRLSVEQGGFISVSHRMQLLRPISVGILTVSDKGSRGEREDLSGPAL
                     ARSVKPLGMFVEEVAIVPDEIGIISDTLTKWSDTGRLNLILTTGGTGLSKRDVTPEAL
                     LSVAEKHVPGIGETMRSVSMKITSKAMLSRGCAVIRGETLIIALPGSEKAAEECFAAI
                     APALRHGIEILCGWDSECGSRPHHHH"
     gene            complement(1398..1922)
                     /gene="moaC"
                     /locus_tag="PK442_06060"
     CDS             complement(1398..1922)
                     /gene="moaC"
                     /locus_tag="PK442_06060"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOO87249.1"
                     /translation="MSIIEDEKNELTHLDEQGRPLMVDVGEKPFSARTAEAEGWVLLD
                     QAICDLLGDKGLSKKGDVLRIAETAGIMAVKKTWELIPLCHSIRVDSVRVQCELLADI
                     RKIHIKSIVKANDVTGVEMEALTGVSVAALTVYDMCKAVSKGMVIDGIRLLRKTGGKS
                     GDYIASTPIQVEDV"
     gene            complement(1930..2988)
                     /gene="moaA"
                     /locus_tag="PK442_06065"
     CDS             complement(1930..2988)
                     /gene="moaA"
                     /locus_tag="PK442_06065"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOO87250.1"
                     /translation="MGRIMRDPYGRTLDYVRISITDRCNFRCLYCMPANGIAWIPHED
                     IMTYEDIFFLVSVLSELGVKRLRFTGGEPFVRKGFVSFLQEIRQRFPSIDVAVTTNGA
                     FLERDASALSALRLHSVNVSLDTLHPQKFADITRTGELGTVLRGIRALVAKGGVSVKI
                     NTVVMRDFNLNEISGLVRFAKENDVLLRFIEFMPLDKDVWSNQQYVSASEILSLLPDA
                     LQWSPSPKSSVTTQNTSSAIPNGPAKYFRNAKTGQDIGIISAVSDHFCHTCNRLRITS
                     TGEVRPCLFSNRGVAIIDALRRKDPDAARDGILMAVKNKPHEGGTGIPEERQNPEDAP
                     QKRTDLFQEERHMSRIGG"
BASE COUNT          708 a          793 c          754 g          736 t
ORIGIN      
        1 ggccgcggca tcctcaaaaa acctggagat cagctctttc cgaattgcag aaagagagag
       61 cgacgaggta tcgatgattg tgtcggcgta gtcgagaatc ggttgtaggc gttcgcgttc
      121 ctttgcgata ccgtcaagaa aagacccgtc gggttccaga ggatgacggc gccgtgtggt
      181 ttcaaaacgc cgcagcaaaa tttcatcctt ggcatcgaaa aagagaatat gcacatgacg
      241 cgtctgtttt ctcagcaagg cgagggccgc cggaaaatca tccagcagcg aatctccgcg
      301 aatatccacg accgctgcga ctccccagcg aacggctgcc cgatgttttt ccagaagaaa
      361 gagcagttga gaaagcagaa caggaggtat gttgtcaatt gcaaacatac cttgatcttc
      421 gaggattttc agcgcggtgg atttccctgc gccggacata cccgtgataa taatgcactt
      481 gttcacaccg acggcatcca tatccacgct tcgtctccct tcatacagat ttgcgcccca
      541 atgtatattt tctctgttat cgggctgctc aatgatgatg atgcgggcgg gagccgcatt
      601 cactgtccca tccgcacagt atctctatac cgtggcgcag tgcgggagct attgcagcga
      661 agcattcttc cgcagccttt tcgctccctg gaagggcaat aataagcgtt tctccacgga
      721 taacggcgca gcctctgctc agcatggctt ttgaggttat cttcatcgaa acgctgcgca
      781 ttgtttcccc gatgcccgga acatgttttt cggcaacgga aagaagcgcc tcgggagtaa
      841 catctctctt ggacagaccg gttccgcctg tagtcagtat gagattgaga cggccggtat
      901 cgctccactt cgtgagcgta tccgagatga ttccgatctc atcagggaca atcgcaactt
      961 cttcgacaaa catcccgagc ggcttgacgc tacgggctag agccggaccg gagaggtcct
     1021 ccctctctcc cctgctccct ttgtcactca cggtgagtat gccgaccgaa atcggacgga
     1081 gcagctgcat tctatgtgaa acggagatga aacctccctg ttcaacgctc aatcgcggca
     1141 acggattccc ttccaatatg cgaaagagaa catcctctcc ggaagagagg aaataggaga
     1201 attttagcgc gtctttggag agaacaaagc cgacgctctt ttccggaagt ttttctctct
     1261 ctcccggaga gagcagaaag aacggctttt ctgtcccgtt caaggcgctt tctccggctt
     1321 tgttaatgct tacgtaactg agcgtgtgcg ggagaaaggt acgggtagac ctttcaataa
     1381 tagcgaggac tttcatgtca aacgtcctcc acttgaattg gagtcgaagc gatgtagtcg
     1441 ccgctttttc ctccggtttt acggagaaga cggatgccgt caataaccat ccctttcgag
     1501 accgctttgc acatatcgta caccgtgaga gccgctactg aaacgccggt aagcgcctcc
     1561 atttcaactc ccgtaacgtc atttgccttc actatgctct tgatatggat ttttcggatg
     1621 tccgccagca gctcgcactg cactcgtaca ctgtcgactc gaatcgagtg acataaaggg
     1681 atcagctccc acgttttttt cacggccatt attccggcag tttcggcaat acgaaggaca
     1741 tctccttttt ttgagaggcc tttgtcgccc aggagatcac agattgcctg atccaacaaa
     1801 acccatcctt ccgcctccgc tgtgcgggcg gaaaagggct tctcgccgac atcgaccatt
     1861 agaggccgcc cctgttcatc cagatgcgtc aattcatttt tttcatcttc aataatgctc
     1921 acagagagtt catcctccga tccgggacat gtgacgttct tcttggaaga gatctgtacg
     1981 cttttgagga gcgtcttccg ggttttgccg ctcttcggga atacccgtac ccccttcatg
     2041 gggtttattt tttaccgcca tgagaatgcc atcgcgggcg gcgtcgggat ccttccttcg
     2101 caaagcgtcg atgatggcga cgcctctgtt gctgaagagg caagggcgca cttcaccagt
     2161 tgatgttatc cgaaggcgat tgcacgtatg gcagaaatga tcggagacag ccgagatgat
     2221 accgatatcc tgccctgttt tcgcattcct gaaatacttt gctgggccgt taggaattgc
     2281 agagcttgtg ttctgcgttg tcacagagct cttcggtgag ggggaccact gaagagcgtc
     2341 cgggagaagc gagagaatct ccgaggcgga gacgtactgc tggtttgacc agacgtcctt
     2401 gtcaagcggc atgaattcga taaacctgag cagtacatcg ttttctttgg cgaagcgaac
     2461 gagcccggaa atctcattga ggttgaaatc acgcattacc actgtgttta tctttacaga
     2521 aactcctccc tttgcaacga gggcgcgaat tccgcggagc actgttccga gttcccccgt
     2581 cctcgtaatg tcagcgaact tctgcgggtg caacgtatca agactcacat tcaccgaatg
     2641 aaggcggaga gcagaaagtg ctgatgcatc ccgttccaga aacgctccgt ttgtcgttac
     2701 cgcgacgtcg atggaaggga atctttgccg tatttcttgc agaaacgaaa cgaatccctt
     2761 cctgacaaaa ggctctccgc cggtgaaacg aagcctcttt actccaagtt cagaaagaac
     2821 ggagacgaga aagaatatgt cttcgtacgt cataatatct tcatgaggaa tccaggcaat
     2881 accgtttgcc ggcatgcagt aaagacagcg aaaattacag cggtcggtga tagaaatacg
     2941 aacgtagtca agcgttcttc cataaggatc acgcattatt ctgcccatcg g
//
LOCUS       DAOSWW010000250         2990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24391_length_2990_cov_3.680409, whole genome shotgun sequence.
ACCESSION   DAOSWW010000250 DAOSWW010000000
VERSION     DAOSWW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24391_length_2990_cov_3.680409"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PK442_06070"
     CDS             complement(<1..498)
                     /locus_tag="PK442_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO87251.1"
                     /translation="MGPIKRVRLSERVTDAIETMIAEGAFAPGDRFLSENDLVKKLEV
                     SRSSVREAVRILEVRGRVIVKHGKGIFIAEPPDEDRDPFTGWLKDNKAPIFDHFEVRL
                     IIEPKAARRAAEKADDESVKGIEKAYSAFAVSAERGDTAAMIRFDGEFHRLVAKATKN
                     RTLYHL"
     gene            complement(602..>2990)
                     /locus_tag="PK442_06075"
     CDS             complement(602..>2990)
                     /locus_tag="PK442_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87252.1"
                     /translation="ARLRGDMATHIGEHVRHYLHIPHIINRLNADALGGGMLNVLDER
                     SLEFHFRKQIDVFPSVSSIYFGNTSGGLVNSGREAPFDSRYVIGTIGSKAGVFRKYSI
                     DKDGNRTGLLAEVPDFDARTRGWYSRAQKSGGEGVWSDVYVLFTGQDMAITASRPVYD
                     AHGGFLGVAAVDLSLSQLTAFLQALPLGEGTAFIMERSGLMLASSRGEPPFVFYEGGE
                     ARRLHAADSVHAEISGAALFMASKLEKNGLLTDGEIAFESGGERCYLRTESLHGPGDL
                     DWIIAVTAPEREFMDRIDKNNRTTTLVILFAVLLSFGAALLLARRMAQPVLRLNAAAR
                     AITRGENPPELPVDCEILEVGELSASFNRMAEALRETIRGLNEEIGVRKETESRVRAA
                     MERAEDLRKEAENANRAKSEFLANMSHEIRTPLNGVVGFLDMLRGPGLDQEQLSYVDN
                     AKVSAYALLEIISNILDISKIEAGKLELETVRTDIPKLLEESLVIAGHGSVSRGVRVF
                     SSVHPPFPRSAYVDPLRLRQVLVNLLGNAVKFTEEGEVELSASFQPSAGAEHMGLFTF
                     SVRDTGIGIRKEDREKLFRPFSQADASNTRKYGGTGLGLVISNGILKKMGSALELESE
                     PGNGSRFSFTVSARYEEDVSLPDTPRESKGEVRQEQAFSELSSRAFSLLIVEDLRMNR
                     TLLRLLASRLLPSAAVFEAENGERAVEIFRTHAPDIVFMDMHMPVMNGFEATAAIREF
                     EKSRGTHTPIVALTADVLSETRERCFQAGMDDFLPKPIEAESLRAVLERYLSLSF"
BASE COUNT          635 a          888 c          796 g          671 t
ORIGIN      
        1 caggtgatag agcgtccggt tcttcgtagc ttttgcgacg agtctgtgaa actctccgtc
       61 gaaacgtatc attgccgcgg tgtctccccg ctccgcgctc acggcaaatg cgctgtatgc
      121 cttttcaatg cctttgacgc tttcgtcgtc ggctttttca gcggcgcgcc gcgcggcttt
      181 gggttctatg atcaggcgca cctcgaaatg atcaaaaatc ggggccttgt tgtccttcag
      241 ccatcctgtg aaggggtcgc gatcctcgtc cggaggttcg gcaataaaaa tacccttgcc
      301 gtgcttcacg atcacccttc cgcgaacctc cagaattcgg acggcctcac ggacggagga
      361 acggctcacc tcaagttttt ttacaagatc gttctcggaa aggaatctgt ccccgggggc
      421 gaaagccccc tctgcgatca tcgtctcaat cgcgtctgtt accctttcgg acaggcgtac
      481 tctttttatg gggcccatag tttcacccgc ttttcgcaag atgagaacat agcggacatc
      541 ttcttatcag acaagttgat tgtacccctg ttttccgcac cctgtcaaga gagtaaaaag
      601 ttcagaaaga caaagaaaga taccgctcca ggacggcgcg cagcgattca gcctctatcg
      661 gttttggaag aaagtcgtcc atacccgcct gaaaacagcg ttcgcgagtt tcggacagca
      721 catccgccgt cagggctact atcggcgtat gcgtcccgcg ggatttctca aactctctga
      781 tggcggcggt cgcctcgaag ccgttcatca ccggcatgtg catatccatg aagacgatat
      841 ccggcgcgtg cgtacggaat atctcaacag cccgctcccc gttttccgcc tcaaaaacag
      901 cggcggaggg gagcaatcgc gacgcaagca gcctcaggag cgtcctgttc attctgagat
      961 cttccactat gaggagcgag aaggctcgtg aagaaagctc ggaaaacgcc tgctcttgtc
     1021 gcacttcacc cttcgactcc cgcggggtgt ccggcaaaga gacgtcctcc tcgtaccgtg
     1081 cggaaaccgt gaaagaaaag cggcttccat tcccgggctc gctctcaagt tcgagagcgc
     1141 ttcccatttt ttttagaata ccattggaga taacaagacc gagtcccgtt ccgccgtact
     1201 ttctcgtgtt cgacgcgtcc gcctgggaaa acggacggaa gagcttctct ctgtcttctt
     1261 ttcggatgcc tattccggta tcacgcacgg aaaaggtgaa aagccccatg tgttcagcgc
     1321 cggctgaggg ctgaaaagag gcggagagtt cgacttctcc ctcctccgtg aatttgacgg
     1381 cgttccccag aagattgaca agcacttgcc gcaaacgcaa gggatccaca tacgcagagc
     1441 gcgggaaggg agggtgaacg ctcgagaaga ctcgaacgcc tctgcttacc gacccgtgac
     1501 ctgcgatgac caaagattct tcgagcagct ttggaatatc cgtccgaacg gtctccagct
     1561 caagtttacc ggcctctatt ttagaaatat cgagaatgtt gcttattatt tccaggagcg
     1621 cgtaggcgga cactttcgcg ttgtcgacgt aagagagctg ttcctggtcg agccccgggc
     1681 cgcgcaacat atccagaaag ccgacaaccc cgttgagcgg cgttctgatc tcatggctca
     1741 tgttcgccag aaattcgctt ttcgccctgt tcgcgttctc cgcttctttt ctgaggtctt
     1801 ccgccctttc catcgcggcg cgcacacggg attccgtctc ttttcgcacg ccgatctctt
     1861 cgttcaagcc gcggatggtt tcacgcagcg cctccgccat cctgttgaac gacgcggaaa
     1921 gctcgcccac ctcgagaatt tcgcagtcga caggaagctc gggcggattc tcccctctgg
     1981 tgatcgcacg cgcggcggca ttcaggcgaa ggaccggctg cgccattctt cgggcaagaa
     2041 gcagcgccgc gccgaaagag aggagcacgg caaagaggat aaccagcgtc gtcgtgcggt
     2101 tgtttttatc aatccggtcc atgaactctc tctccggagc cgtaacggcg atgatccagt
     2161 caagatctcc cgggccgtgc agggattctg tgcgaagata gcagcgttcc ccgccgcttt
     2221 caaaagcgat ctccccgtct gtgagcaaac cgttcttttc gagtttactc gccatgaaaa
     2281 gagcggctcc actgatttcg gcatgcacgc tgtcggcggc atgcaggcgc cgggcttctc
     2341 ctccttcgta gaagacaaat ggaggttccc ctctcgaaga ggcaagcatc agtcccgagc
     2401 gctccatgat gaacgcggtt ccctcgccga gcggcagcgc ctgcaaaaat gccgtcagct
     2461 gcgaaaggga gaggtccacg gccgctacgc cgagaaaacc tccatgagcg tcgtacaccg
     2521 ggcgactggc ggtaatggcc atatcctgac ctgtaaagag aacatacacg tcgctccaga
     2581 ctccctcgcc tccgctcttt tgagcacggg aataccatcc tctcgtccgc gcgtcaaaat
     2641 cggggacttc ggctaaaagc ccggtgcggt tgccgtcttt gtcgatggag tacttccgga
     2701 agacgcccgc tttgctaccg atcgtgccga tgacgtagcg ggaatcaaaa ggagcttctc
     2761 tgccgctgtt gaccagtcct cctgacgtat tgccgaagta gatgctcgaa actgaaggga
     2821 aaacatctat ctgcttcctg aaatggaatt ccagcgagcg ctcgtcgaga acattcagca
     2881 ttcccccgcc caaagcatcc gcgttcaggc ggttgataat atgaggtatg tggagataat
     2941 gacgcacgtg ctcgccgata tgcgtcgcca tatcgcctcg cagcctggcg
//
LOCUS       DAOSWW010000251         2981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24517_length_2981_cov_3.616541, whole genome shotgun sequence.
ACCESSION   DAOSWW010000251 DAOSWW010000000
VERSION     DAOSWW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24517_length_2981_cov_3.616541"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2981
                     /locus_tag="PK442_06080"
     CDS             <1..>2981
                     /locus_tag="PK442_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87253.1"
                     /translation="LLTAKFPSLLATEGLRPGQPALLKVDEARFGRRTSRVANLPAPA
                     RVSLALRFADRAPDKSGVAWMPVNPGTREALYLVGMRTEALDKLADELLERYPNPPAE
                     IGEQLSGHTQQGRFIAAQWAMVASEFGEEAAGAALRIPDITKRIGMINGIPLSVAAPG
                     EKIKGFVRDAVLVDGVELPKAASDGWNYALAKHLTSQLGAGDGAYKINVAGHSDGSVK
                     GASQITAAKPAGISDAVLEEHAEAQAELRRLMGDNAFDASGLDSQILAIDAEIRAQLD
                     EEYRGFVMDAGGEKVGGTEGAVELNFTLGGKNITIRGRKAWFASEHLSKASDVDAKAD
                     RYQSAFGVSALFNIGVFAPKSAERIRGLLRLGYENLAKMPEEAYAKLLGAAMASSAAK
                     STPASVYVVEASGLAMDNHMLQSGYAGAALVREEARPRVQALYNLVAPSMQTFEEAVS
                     GASPYLKAAVNGQPARIRLNDTRDDAMFSVWTTKEFSEKFTTEAEQRKALREFLTTLS
                     EFRINTLSARERRTMPEEALKLAEAEQKKRADKLRGMSRYFRDFTQAQSLTLLEDASG
                     FFRADGSFREETIYAAIGRDGETRLVAAPRQGMAERFPGAEGMAARDAPLVTAPATLA
                     VRSVRVLGEDGRIKRVRRVASGTQNLLVMDVASLKNRGIDNDADKHKMNFAVRPRGAA
                     PSLSEVKKRASAGELTKNDFAWLGYWVASEFIDALDGQPDFVANAALEPDFGNGALLT
                     IPAWLKRVAPAEFEGRDKATGREMLETKLHPLAALSYPVWEARGMAAAEASDRGHVVQ
                     ANDGMAAVAEHRMDLSAEPGKFGADLVVKDIAPGLVLGVSGARWADLDDKLQRVYDLG
                     NNVMNATIDAEKNWTEFLHGVMADTSPMKYALVASWTPRFSRAEELKKREAFITEALV
                     AGKKKEEGVEAYESWARSTPGTTLHFEQSWVDFVETDPGMRLFLETARERGARRGRRC
                     GRLGRRAVLAEVEVEVEAEVE"
BASE COUNT          574 a          882 c         1064 g          461 t
ORIGIN      
        1 cattgctcac cgccaagttc ccaagtctct tggcgactga ggggcttcgt cccgggcagc
       61 ccgctctgct gaaggtcgac gaggccaggt ttgggcgcag gacgtcccgc gtagcaaatc
      121 tcccggcccc cgcgcgagtc agcctagccc tgcgattcgc tgacagggcc ccggacaagt
      181 ccggcgtggc gtggatgccg gtgaaccccg gcacacgcga ggcgttgtac ttggttggca
      241 tgcggacgga agcgctggac aagctggcgg acgagctgtt ggagcggtat ccgaaccccc
      301 ctgccgagat cggggagcaa ctgtccgggc acacacagca gggaagattc attgccgccc
      361 agtgggccat ggtggccagc gagtttggtg aagaggccgc cggggcggcg ctgcgaatcc
      421 ccgacatcac gaagcggatc ggaatgatca acgggattcc tctgtccgtc gcagccccgg
      481 gggagaagat caagggcttc gtccgggacg cggtgctcgt cgacggggtc gagctgccaa
      541 aggccgcgtc cgatggctgg aactacgccc tcgcgaagca tcttacgagt cagctcggcg
      601 cgggcgacgg cgcctacaag atcaacgtgg cggggcactc cgacggctcc gtgaagggcg
      661 cctcgcagat caccgcggcc aagcccgccg gtatctccga cgccgtgctg gaagagcacg
      721 cggaggccca ggcagagctg cgtaggctaa tgggcgacaa cgcgttcgac gcctccgggc
      781 tggatagcca gatcctcgcc atcgacgcgg agatccgggc ccagttggac gaggaatacc
      841 gcgggtttgt gatggacgcc gggggcgaaa aagtcggtgg gacggaggga gccgtcgagc
      901 tgaacttcac gctcgggggt aagaatataa cgattcgcgg gcgcaaggcc tggttcgcaa
      961 gcgagcacct ttcgaaagca tcggacgtcg acgcgaaggc agaccggtac cagtccgcct
     1021 tcggggtaag cgcgctgttc aatatcggag tgttcgcgcc aaagtcggct gagcgcattc
     1081 gcgggctgct tcgcctcggg tacgagaacc tagccaagat gccggaagaa gcgtacgcca
     1141 agctgctcgg agcggccatg gcgtcgtcgg ctgcgaagtc gactccggct tccgtatatg
     1201 tcgtcgaggc gtccggactc gcgatggaca accacatgct gcagtccggg tacgccggcg
     1261 ccgcgctggt tcgcgaagag gcccgcccgc gtgtccaagc attgtacaac ctggtggctc
     1321 cttccatgca gacgttcgaa gaggcggtat ccggggcgtc gccgtacttg aaggcggcgg
     1381 tcaacggaca gcccgcccgg atacggctca acgacacacg ggacgacgcc atgttctcgg
     1441 tgtggacgac gaaagagttt tccgaaaagt ttacgacgga agcggagcag cggaaggcgc
     1501 tgcgggagtt cctgacaacg ctgtccgagt tccgtatcaa tacgctatcc gccagagagc
     1561 gacgcacgat gccggaagag gcgctgaagc tggccgaggc ggagcagaag aaacgcgcgg
     1621 acaagctccg cggcatgagc cggtacttcc gggacttcac gcaggcccaa tcgctgaccc
     1681 ttctggagga cgcttcggga ttcttccggg cagacggctc cttccgggaa gagactatct
     1741 atgcggcaat cggccgggac ggggagacga ggcttgtcgc cgccccgcgc cagggaatgg
     1801 ccgaacggtt ccctggggcc gaggggatgg ctgcgagaga cgccccgctt gtcacggctc
     1861 ctgctacgct cgcagtgcgc tccgtccggg tgctggggga agacggccgc atcaagcgtg
     1921 tccgccgggt ggcgtctggg acgcagaacc tacttgtgat ggacgtggct tctctgaaga
     1981 accgcggcat cgacaacgac gccgacaagc acaagatgaa tttcgcggtg cgccccaggg
     2041 gcgcggcgcc gtctttgtcc gaagtgaaga agcgcgcctc cgcgggcgag ctcaccaaga
     2101 acgactttgc gtggctggga tactgggtgg cgtccgagtt catcgacgcg ctcgacggcc
     2161 agccggattt cgtggcgaac gcggccctgg agccagattt tgggaacggg gcgctgctca
     2221 ctatcccggc ctggctgaag cgtgtggccc cagccgagtt cgaaggccgc gacaaagcga
     2281 ccggccggga aatgctggag accaagctcc accccctggc ggcgctatcc tatccggtat
     2341 gggaagcgcg tggtatggcg gcggcggagg cgagtgatcg tgggcacgtg gtgcaggcca
     2401 acgacggcat ggccgctgtg gcggagcacc ggatggacct gtctgcggag cccggcaagt
     2461 tcggtgcgga cctcgtcgta aaggacatcg cccccgggct tgttctcgga gtcagtggcg
     2521 cgcgttgggc cgacttggac gacaagctgc agcgcgtgta cgacctcgga aacaacgtga
     2581 tgaacgcgac catcgacgcg gagaagaact ggacggagtt cctccatggg gtgatggcgg
     2641 atacgagccc gatgaagtac gcgctcgtcg cttcgtggac gccgcgcttc tccagggccg
     2701 aagagctgaa gaaacgagag gccttcataa cggaggccct ggtcgccggg aagaaaaaag
     2761 aagaaggagt ggaggcctac gagagctggg cccggagcac gccggggacc accctgcact
     2821 tcgaacagtc gtgggtcgac ttcgtggaga cggaccccgg aatgcggctc ttcctggaga
     2881 cggcgaggga gcgaggcgcc cggaggggca gaagatgcgg gcggctgggc cgccgcgccg
     2941 ttctcgccga ggtagaagta gaggttgaag ccgaggtaga a
//
LOCUS       DAOSWW010000252         2980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24534_length_2980_cov_2.532650, whole genome shotgun sequence.
ACCESSION   DAOSWW010000252 DAOSWW010000000
VERSION     DAOSWW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24534_length_2980_cov_2.532650"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1316)
                     /locus_tag="PK442_06085"
     CDS             complement(33..1316)
                     /locus_tag="PK442_06085"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO87254.1"
                     /translation="MLWLFLGILFFFTIFGVPIVFCLGIANIVMLLVMDIPLMALPAK
                     VIQGADSFPLLAIPFFMFVGEVMNRGGIARRLVDFADALVGHITGGLGHVSILSSMFF
                     GGICGSAVAETAAIGGLLIPPMKKQGFPAPLAAAICASAAVIGIIIPPSIPFILFGII
                     TSTSIARIFLGGIIPGIVVGLALMVTTYFITKKKGIARPAPDRKFSVKRVGQTLLSAG
                     WALSIPVIIVGGILGGIFTATEAGAISAFVAMILGLFVYKEITFKDLPGIFLSTGKTT
                     ATVLYLCGMAMCTAWLLTRARVPFELAAMVQSITDSQLGVLLMTNVLLFLVGFVMDLT
                     PAMLILAPILLPVMQQAGVDPVYFGVIMSVNLGIGLITPPVGTVLYVASGVANVKFEE
                     LVKSIAPFVVAELAVLILLIFFPQIVMFIPNLVLP"
     gene            complement(1321..1815)
                     /locus_tag="PK442_06090"
     CDS             complement(1321..1815)
                     /locus_tag="PK442_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO87255.1"
                     /translation="MKRLLEVGASFYKLLLTIMTVALFIIVGVSVFTRYCLNSSLGWS
                     DELSRFIFIWVTFLGAAYAYGLDEHIGLDFVVDRIRSDRVRTVVRLLSEIAIGCVILV
                     ITWHGWEVAFSATNLSPALDIPMTWVYIVVPLTGCLMVVMNILKIGKWIGMLLATPSA
                     PAGR"
     gene            complement(1812..2081)
                     /locus_tag="PK442_06095"
     CDS             complement(1812..2081)
                     /locus_tag="PK442_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87256.1"
                     /translation="MVDRNEIEDLTMAAHIVHVGRIIRDKGRGIMVCPCIDDATSKKL
                     GFFPAATPQQALDLAFSMTDPDATVIVLQNGGDLLPIHEPRRDAR"
     gene            complement(2051..>2980)
                     /gene="larA"
                     /locus_tag="PK442_06100"
     CDS             complement(2051..>2980)
                     /gene="larA"
                     /locus_tag="PK442_06100"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOO87257.1"
                     /translation="VRRALAEPIASPGLKDMVKQGDSALLLIDDNTRNTPASRILPLV
                     LEELAAGGVQIQDTAILIALGTHRHMTDAEVEAKVGAELAGRLRIFQHDAHDESGLAH
                     IGVTEHGTDVWVNKLLLEFDHVIAVGHITPHRVAGFSGGAKMVQPGVSGVVTTGQTHW
                     ISALYDGAEIMGTIDNPVRREMNAVAKKAGLSFIVNVVLDREERIYRCVCGDPEKAHA
                     AGCEASREIFGVLFPEYADIVVTDTFPADSELWQAAKGIYAGDLPLKRGGVLITVTPC
                     PEGVAQGHPELTEIGTSPSPKSRKWWTETRSRT"
BASE COUNT          595 a          905 c          975 g          505 t
ORIGIN      
        1 ccccttccgg atgtcagaaa aaatgacgcg ctctacggaa gcacaagatt cgggatgaac
       61 atgacgatct gcgggaagaa gatgagcagg atgagcaccg ccagttccgc gacgacgaag
      121 ggagcgatgg atttcaccag ctcctcgaac ttcacgttcg cgactccgct cgctacgtag
      181 aggactgtgc ccacaggcgg agtgataagc ccgatgccga ggttcaccga catgatcacg
      241 ccgaaataga ccggatcgac ccccgcctgc tgcatcaccg gaagcaggat cggcgcgagg
      301 atgagcatcg ccggggtgag atccatgacg aagccgacga ggaagaggag cacgttcgtc
      361 atcaggagga cgccgagctg cgagtcggtg atcgactgga ccatcgccgc gagctcgaag
      421 ggaacccgcg cgcgggtgag gagccacgca gtgcacatcg ccataccgca gaggtagagc
      481 accgtggcgg ttgtcttgcc tgtcgacaaa aagatgccgg gcaggtcttt gaacgtgatc
      541 tccttgtaga cgaagagacc gaggatcatg gcgacgaacg ccgagatcgc gcccgcctcc
      601 gtcgcggtga agatgccgcc gagaatgcct cccacgatga tgaccggtat ggagagcgcc
      661 caccccgcgc tcaggagagt ctggccgacg cgtttgaccg aaaacttccg gtccggagcg
      721 ggcctcgcga tccccttttt cttcgtgatg aagtaggtgg tcaccatcag ggccagtccg
      781 acgacgatgc ccggaatgat gccgccgagg aagatgcggg cgatcgaggt gctcgtgatg
      841 atgccgaaga ggatgaaggg gatgctcggc ggaatgatga tgccgatgac cgccgccgac
      901 gcgcagatcg ccgccgcgag cggggcgggg aagccctgct tcttcatcgg cgggatgagc
      961 agaccgccga tcgccgccgt ctcggctacc gccgaaccgc agatgccgcc gaagaacatg
     1021 ctgctcagga tggagacgtg ccccaggccg cccgtgatgt gccccacgag ggcgtccgcg
     1081 aagtccacaa gcctgcgggc gatgccgccc ctgttcatca cctcgcccac gaacatgaag
     1141 aaggggatgg cgaggagcgg gaagctgtcg gctccctgga tgaccttcgc ggggagcgcc
     1201 atcaggggga tgtccatcac gagcagcatc acgatgttcg cgatgcccag gcaaaagacg
     1261 atgggaacgc cgaagatggt aaagaagaag agaatgccga gaaagagcca gagcatggcg
     1321 ttacctcccc gccggggcgg acggcgttgc gagcagcatg ccgatccact tcccgatctt
     1381 caggatgttc atgaccacca tcaggcaccc cgtaagcggg acgacgatgt agacccaggt
     1441 cattgggata tcgagcgcgg gagagaggtt cgtcgcggag aaggcgacct cccagccgtg
     1501 ccaggtgata acgaggatga cgcagccgat ggcgatctcg ctcaggaggc ggacgacggt
     1561 gcggactcgg tcgctcctga tgcggtccac gacgaagtcc agtccgatgt gttcgtccag
     1621 tccgtaggcg tacgccgccc cgaggaaggt cacccagatg aagatgaagc gggacagctc
     1681 gtccgaccat ccgagcgagc tgttgaggca gtagcgcgtg aagacgctca cgccgacgat
     1741 gatgaagagc gccacggtca tgatggtgag caggagtttg tagaatgacg cccccacctc
     1801 cagcagccgc ttcatcgtgc gtccctccgg ggttcgtgaa tcgggagcag atcgccgccg
     1861 ttctgcagga cgatcaccgt agcgtccggg tcggtcatcg agaaggcgag atcgagcgct
     1921 tgctgcggcg tcgcggcggg gaaaaagccg agtttcttcg aggttgcgtc gtcgatgcag
     1981 gggcagacca taatcccccg ccccttgtcg cggatgatcc gcccgacgtg gacgatatgc
     2041 gcggccatcg tcaagtcctc gatctcgttt ctgtccacca tttccttgac ttcggagaag
     2101 gggaggtacc gatctccgtg agttccgggt gcccctgcgc gacgccctcg gggcaggggg
     2161 tgaccgtgat taaaacgccg ccgcgcttca acgggaggtc tcccgcgtag atccccttcg
     2221 cggcctgcca cagttcgctg tctgcgggga atgtgtccgt aaccacgatg tcggcgtact
     2281 cggggaacag aacgccgaag atctcccgcg acgcctcgca gcctgcggcg tgcgctttct
     2341 ccgggtcgcc gcagacgcag cggtagatac gctcctcccg gtcaaggacc acgttcacga
     2401 tgaaagagag ccctgctttc ttcgccacgg cgttcatctc gcggcgcacg gggttgtcga
     2461 tggtccccat gatctcagcg ccgtcgtaga gcgcgctgat ccagtgcgtc tgccctgtgg
     2521 tgaccactcc ggagacgccc ggctggacca tcttcgcccc tccggagaaa cctgcaaccc
     2581 tgtgcggggt gatgtgtccc actgcgatta cgtggtcgaa ctcaagaagc agcttgttca
     2641 cccagacgtc ggtaccgtgt tcggtgaccc cgatatgggc cagcccgctc tcgtcgtggg
     2701 cgtcgtgctg gaatatccgt agacgtccgg cgagttccgc gccaaccttt gcctccacct
     2761 cggcgtccgt catgtggcgg tgcgttccga gggcgatcag aatggccgtg tcctgaatct
     2821 gcacaccgcc tgcggccaac tcctccagga caagcgggag gatacgagag gcgggggtgt
     2881 tgcgggtgtt atcgtcgatg agcagcaacg ccgaatcccc ctgcttcacc atgtccttca
     2941 gcccggggga ggcgatcggt tcggcaagag cccgtcgtac
//
LOCUS       DAOSWW010000253         2976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24573_length_2976_cov_2.991784, whole genome shotgun sequence.
ACCESSION   DAOSWW010000253 DAOSWW010000000
VERSION     DAOSWW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24573_length_2976_cov_2.991784"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PK442_06105"
     CDS             complement(<1..309)
                     /locus_tag="PK442_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOO87258.1"
                     /translation="MSGIRKSMSPLEWLLLLFLSVLWGGSFFFVGIVVHALPRFTIVA
                     LRVGLAALALHILLLFRGERIPLDKRLWGAFFVIGLLNNVIPFSLIIQGQKHIASGLA
                     S"
     gene            complement(552..1031)
                     /locus_tag="PK442_06110"
     CDS             complement(552..1031)
                     /locus_tag="PK442_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87259.1"
                     /translation="MSGRDDDLLAKELEKLAAAGGKIAAKISGKSSPAVTAEDSEIMR
                     KAARRLDTDQYREERMIEGDARSVLQLAYDALKNTGRILEENERESSVNPRISGVVGA
                     GFFGKNPAVVHAEIVGADERRCTLRLTGAAKEGLIKQGTARKAVEKVLREFDSLKSS"
     gene            complement(1070..2257)
                     /locus_tag="PK442_06115"
     CDS             complement(1070..2257)
                     /locus_tag="PK442_06115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO87260.1"
                     /translation="MTSSDFEYENDPQLRESIERSILLGNLRRMQRLLWGLALFLALV
                     LLCSPFLYEKSVQHFGSAPPLEVQILWRSAWLAFDLFALFGIRRILSHPPERIDLFHR
                     AVETGVPLMNMLFVVFLLTAGYAYNPSLEILYIALFAMAPLIRLNTAKTFIVVGMPLG
                     LFVALMDRRAAGVAHMSNMISLTAVVILAAIVFREMYRSTVREMEQQAIIERQLKIME
                     TFANTDGLTGLANRRSLDENLESEWRRGMREHNFLAVVMLDIDLFKSYNDQYGHPSGD
                     ECIRNIASVIKGYARRAGDIAARYGGEEFVIVLPGVDLGDAASIAEKIRLEIFSANRP
                     HAGSPEGRVTVSAGVASAPAASLDSGEALLRSADLALYRAKAEGRNRVVAASESARVV
                     AEK"
     gene            complement(2581..>2976)
                     /locus_tag="PK442_06120"
     CDS             complement(2581..>2976)
                     /locus_tag="PK442_06120"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOO87261.1"
                     /translation="LDVIGKRLEQPFSGPRELHAAMTRRLELTPDQKGKVEAITKETA
                     PKMHAFAKTLFDQVKSEHAALANGQMSDEQLAESAGKIADLAEGALMEMGRVRAQVWE
                     TLTPEQREEMGKMRHPMGGMERRPGRMRK"
BASE COUNT          619 a          964 c          798 g          595 t
ORIGIN      
        1 cgaggcaaga ccgctggcga tgtgcttctg cccctggatt atcaggctga aggggatcac
       61 gttgttcaga agccctatca cgaagaaggc gccccagagc cgtttgtcca aaggaatgcg
      121 ctcccccctg aagagaagaa ggatatggag cgcaagcgcc gccaggccga cgcggagcgc
      181 gacgatggtg aagcgcggca gggcgtggac cacgatgccg acgaaaaaga acgagccgcc
      241 ccaaagaacc gaaagaaaga gcagcaacaa ccattcgagc ggactcatcg actttctgat
      301 gccgctcatg acgccgctcc ttccccgagc cacggcctgc aggctgccga gcgcccacaa
      361 gaacaacaaa agagacaccc ggtcggaaga agagacgtca acagaggacg gatgcggcgc
      421 gttcgcttca tgcccgctct gtatcggaca ctgtttcgtt tcactcgcat aggaacctcc
      481 cgaaatagag aatgatgctt gattataccc tccgtgcaca cggaacggga ggcgtcctcc
      541 gggagattcc tctacgaaga cttcagggag tcgaactccc gcaatacctt ctcgactgct
      601 tttcttgcag tcccttgctt gatcagtcct tccttggccg ccccggtgag gcgcagagta
      661 caacgcctct catccgcacc gactatctcc gcgtggacca ccgcaggatt tttcccgaag
      721 aaacccgcgc ccaccacacc ggaaatccgt ggattcacgg agctttcacg ctcgttttcc
      781 tccaggattc ggccggtgtt tttcaaggcg tcgtaggcga gttgcaacac gctcctcgca
      841 tccccctcga tcatacgctc ctctctgtac tgatcggtat ccaatcgccg agccgctttg
      901 cgcatgatct cggaatcttc cgcggttacg gcaggacttg acttgcccga tatcttcgcc
      961 gcgatcttcc cgcccgccgc cgcaagcttt tcaagctcct tcgccagaag atcatcatca
     1021 cgtccagaca tacgcgacac ctccatcagc gttgttcaca ctccatgcct cacttttccg
     1081 caacaacacg cgcactctcg cttgccgcga ccaccctgtt gcgcccttcc gccttcgcac
     1141 ggtacaacgc caggtccgcg gaacgaagaa gcgcttcgcc ggagtcgaga gaggccgccg
     1201 gagcggaggc cacccccgcg ctgacggtca cccggccttc cggcgacccg gcatgcggac
     1261 ggtttgcgga gaatatctcc aggcgaatct tctccgctat cgacgcggcg tccccgagat
     1321 cgacgcccgg cagcacgatc acgaattcct cgccgccgta gcgcgcggct atgtcccccg
     1381 ctctccgcgc ataccccttg atgacggagg cgatgttcct gatacattcg tctccggacg
     1441 gatgtccgta ctgatcgttg tagctcttga aaagatcgat gtccagcatg acgactgcca
     1501 ggaagttatg ttctcgcata ccccttctcc attcgctctc cagattctcg tcgaggctcc
     1561 tgcgatttgc gagcccggtc agtccgtccg tgtttgcaaa cgtttccata atcttgagct
     1621 gacgctcgat gatcgcctgc tgctccattt cgcggacggt ggaacggtac atctctctga
     1681 agacgatcgc cgcgagaatg acgacggcgg tcaggctgat catgttggac atatgcgcga
     1741 ctccggccgc gcggcggtcc atgagcgcca caaacagccc cagcggcatc ccgacgacga
     1801 tgaatgtttt ggctgtattc aaccgtatga gaggcgccat tgcgaaaagg gcgatataca
     1861 gaatttccag agaggggttg tacgcatacc ccgccgtcaa cagaaaaacg acgaagagca
     1921 tgttcatgag cggaacgccc gtctccaccg ctctgtggaa aaggtcgatc cgctccggcg
     1981 gatgcgaaag aatgcggcgg ataccgaaaa gcgcgaagag gtcaaaagca agccaggcgc
     2041 tgcgccagag aatctgcact tcaaggggag gtgcggatcc gaagtgctgc accgactttt
     2101 cgtacagaaa aggactgcag aggagcacca gcgccagaaa gagcgcaagc ccccaaagaa
     2161 ggcgctgcat ccttcgcaga ttgcccagga gaatgctgcg ctcgatcgac tcccggagtt
     2221 gcggatcgtt ctcgtattcg aagtcggagg aggtcatcgt atcgctcctc cccacggagg
     2281 gaacgccgcg tcgctcccgg tatgttcaca gcccctattc ataacacgcc ccccttgaag
     2341 tatcttctgt gcacagtgta tctttggagg tgtgcgctgt caaacaaagg aatatctccc
     2401 aaatactcag gtagaggcgc cgccggagac gagggaaata tgcaaaagcg tccgaggaac
     2461 ctctccctcg aacgcctttc aaaaaacgaa cggaacgctt ctcgcgcgtc gttttttcag
     2521 tctgtacgcg gcgcggatca agatcccgcg ccgcgcgcag cacttcgttc cgcgaagctg
     2581 ctacttccgc atcctccccg gcctgcgctc cattccgccc atgggatggc gcatcttccc
     2641 catctcttcg cgctgctcgg gagtcaacgt ctcccacacc tgcgccctca ctctgcccat
     2701 ctccatcaga gctccttcgg caagatcggc gatttttccg gccgattccg cgagctgctc
     2761 gtcgctcatc tgtccgttcg caagcgccgc atgctccgac ttcacctggt cgaacagcgt
     2821 cttcgcgaag gcgtgcatct tgggcgcggt ctccttcgta attgcctcga cctttccttt
     2881 ctggtcgggc gtcagttcaa ggcggcgcgt cattgcggcg tgcagttccc gaggcccgct
     2941 gaaaggctgc tccaaccgtt tcccgatgac gtcgag
//
LOCUS       DAOSWW010000254         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24696_length_2967_cov_4.258929, whole genome shotgun sequence.
ACCESSION   DAOSWW010000254 DAOSWW010000000
VERSION     DAOSWW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24696_length_2967_cov_4.258929"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..499
                     /locus_tag="PK442_06125"
     CDS             59..499
                     /locus_tag="PK442_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87262.1"
                     /translation="MDNVLRGSGTHFPRWMRWTGMGTGIFLLADGMRRLFAGGGDLSG
                     VFAYILIGTACIYIAGYEKKIFLSEKGFEREILFWGRGRKEQVLWMDMEELILVPSGK
                     GTAVLIPLKQRGWRAFFPGIREDDVRKFVARFREDLPVRKGSGS"
     gene            577..1680
                     /gene="tal"
                     /locus_tag="PK442_06130"
     CDS             577..1680
                     /gene="tal"
                     /locus_tag="PK442_06130"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOO87263.1"
                     /translation="MVQQNRILQALALGQSMWCDFLSRDLLRSGGLDRMIEQGVRGVT
                     TNPSIFENAIARTSDYDTDIQAMMREKKTEEEIYTALTIADVQEGADRFKTLFETSNG
                     VDGFVSLEVSPLLCHDEKGTVEEALRLFEQLDRKNVMIKIPATSAGMKAIRRCISLGV
                     NVNATLIFSRAQYRQVAEAWLSGLEERAAAGESVKGIASVASLFVSRVDTAVDKLLET
                     TGEKDLAGKIAVDNARAAYSDFEAIAASARWKTLAEKGALLQRPLWASTGTKNRAYSP
                     TLYVDSLIGPHTVNTLPPATLEAFLESGEAALTVTSDKEGRSERLARLASCGVNLESV
                     TDTLLQEGLVAFEKAYRVLLEEIGKKGNLLSSK"
     gene            1732..1807
                     /locus_tag="PK442_06135"
     tRNA            1732..1807
                     /locus_tag="PK442_06135"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1765..1767,aa:Ala,seq:ggc)
     gene            1896..2309
                     /gene="speD"
                     /locus_tag="PK442_06140"
     CDS             1896..2309
                     /gene="speD"
                     /locus_tag="PK442_06140"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOO87264.1"
                     /translation="MSPKGFHFIVEASGCGPIISQVDRLQEILVEAAKRANTQVWSVS
                     FHRFPPDGVSGVVVISESHLSIHTWPEVGYMALDIYTCGEESKPQIAVDYVLEQIGAK
                     HTHITEITRGLDDGDMEYYHSFVTWQEVLQRNSNN"
     gene            2552..>2967
                     /locus_tag="PK442_06145"
     CDS             2552..>2967
                     /locus_tag="PK442_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO87265.1"
                     /translation="MNEKIRSLTKKYEEKMLELRRRIHKHPELSGEERETAALVASVL
                     EESGVEVQSDVGGHGVVGVLRGSAKGPCVALRADMDALPMEEKTGLPWASEIKGIIHA
                     CGHDSHTAMLLGAMLVLAEMKNELPGTVKCIFQPAE"
BASE COUNT          741 a          711 c          831 g          684 t
ORIGIN      
        1 caaggctcgg tatcggtata ccttggattg aagccggcga aaagaaggct gaataggcgt
       61 ggacaacgtg cttcggggga gcggaacgca tttcccccgc tggatgcggt ggactggaat
      121 ggggacgggc atctttctcc tggcagacgg aatgcgccgt ctctttgccg gcggcggcga
      181 tctctcgggt gtttttgcct atattctgat cggcacagcg tgtatctaca ttgccggata
      241 cgaaaagaag atcttcctct cagagaaggg gtttgagaga gagattcttt tctggggacg
      301 gggccgcaaa gaacaggttc tctggatgga tatggaggaa ctcatcctcg ttccatcggg
      361 gaagggaacc gccgttctta tccccctgaa acagagaggg tggagggctt ttttcccggg
      421 aattcgcgag gatgatgtga gaaaattcgt agctcgcttc agagaagacc ttcccgttcg
      481 gaaagggagc ggttcataag aaaattgtga acggcgcgga ggaggagact tcccgtctcc
      541 tccaatttct ccactctcta tgaaaggata gattccatgg ttcaacagaa taggatattg
      601 caggcgctgg cgcttggaca gagcatgtgg tgcgacttcc tgagcagaga tctgctccgt
      661 tccggaggtc ttgacaggat gatcgaacaa ggagtgcggg gagtaacaac gaatccctcc
      721 atcttcgaga acgcaatcgc caggacatcc gattacgaca cggatattca agcgatgatg
      781 cgcgagaaaa aaacggaaga ggagatctat actgcactca ccatcgccga tgtgcaggaa
      841 ggagccgacc gtttcaagac gcttttcgag acaagcaacg gggttgacgg ttttgtaagt
      901 cttgaggtaa gtcctttgct ctgtcacgat gaaaaaggaa ctgttgaaga agccttgcgt
      961 ctcttcgaac agcttgacag gaaaaacgtc atgatcaaga taccggcgac atctgccggt
     1021 atgaaagcca ttcgtcgctg tatctctctg ggagtgaacg tcaacgcaac gctcattttt
     1081 tcccgggcgc agtaccgaca ggttgcagaa gcatggcttt cgggtcttga agaaagagct
     1141 gcggcaggcg aatcggtaaa gggcatcgcc tcggttgcct ctctttttgt cagtcgagtt
     1201 gacaccgcgg tggacaagct tctcgaaaca acgggagaaa aggatcttgc cggtaaaatc
     1261 gcagtcgaca acgcccgtgc cgcatattcc gactttgaag cgatagcggc atccgcacga
     1321 tggaaaactc ttgcggaaaa gggtgctttg cttcagcgcc cattatgggc cagcacggga
     1381 acgaaaaacc gcgcctactc cccgacgctc tacgttgact ctcttatagg tcctcatact
     1441 gtgaatacgc tgcctccggc gacccttgaa gcgttccttg agagcggaga ggccgcgctg
     1501 acggttactt ccgacaagga aggacgatcg gaacgattgg ctcgtcttgc ctcatgcggt
     1561 gtcaatctgg aatccgtaac ggataccttg ctccaagaag ggcttgtcgc ttttgaaaaa
     1621 gcgtacagag ttcttctgga ggagatcggg aaaaaaggaa atcttctttc ttccaaataa
     1681 gctgttgaca tattaggcgc cgctccttat aatactcccg tttgcgcata cggggctata
     1741 gctcagttgg gagagcgctt gaatggcatt caagaggtca ggggttcgaa tccccttagc
     1801 tccaccattt acgcaggatc aggccgtccg gtgagatcgg acggccattt ttcttccctt
     1861 tgattacaaa tacactcaga aagaggagga aaacaatgtc accgaaaggg ttccatttca
     1921 ttgtggaagc atcagggtgc ggaccgatta tttctcaggt ggacaggctg caggagatac
     1981 ttgtcgaagc cgcaaaacga gcgaatactc aggtctggtc ggtctccttt catcgctttc
     2041 ctcccgacgg cgtgagcggt gtcgtcgtca tcagcgaatc acatctctct attcatacat
     2101 ggcctgaagt cggctacatg gcgctggata tttacacgtg cggcgaggaa agcaagccgc
     2161 agatcgccgt tgattatgtg cttgaacaga taggcgcgaa gcatacgcat attacggaga
     2221 ttacacgcgg cctcgacgac ggcgatatgg agtattatca ttcctttgtt acctggcaag
     2281 aggttcttca gcgcaacagc aacaactaat agaaagaccc cgtggaaacc ttcaggtttg
     2341 tacggggtct ttttctgtat acccgcctcg cgcatcatgc atcgttgaaa atatccggcg
     2401 tctatgttac cttatcacgg aagtctttca aaattgtcca ttagcggttc aacatccggg
     2461 attgcgctga aaaccgctgt cagctgtctt tcgcacgttg aacagccttc cgtgaaaaaa
     2521 gcagaaatga attcaacagg aggaaccccc agtgaacgag aaaatacgtt cgttaacaaa
     2581 aaaatacgaa gagaagatgc tggagctccg ccgccgcatt cacaaacatc ctgaattgag
     2641 cggtgaggag cgcgaaaccg cggcgctggt agcgtccgtt cttgaagaat ctggagtcga
     2701 agtgcaatcc gatgtcgggg ggcatggcgt tgtcggtgtg cttcgaggga gcgcaaaagg
     2761 tccctgcgtc gctcttaggg cggatatgga cgccctccca atggaggaga aaacagggct
     2821 tccctgggcg tcggaaataa aaggaataat acacgcatgc gggcatgatt cgcataccgc
     2881 aatgcttttg ggtgcgatgc ttgtacttgc cgagatgaaa aatgagcttc ctggtacagt
     2941 caagtgtatc tttcagcccg ctgagga
//
LOCUS       DAOSWW010000255         2959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24788_length_2959_cov_3.723485, whole genome shotgun sequence.
ACCESSION   DAOSWW010000255 DAOSWW010000000
VERSION     DAOSWW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24788_length_2959_cov_3.723485"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PK442_06150"
     CDS             complement(<1..748)
                     /locus_tag="PK442_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87266.1"
                     /translation="MSQGKFQMQKMLTRYGTILALIVLCAGFSFAVPGFLAGENGMNI
                     LRQIALLAVISEGFTMCLIVGELDLAFPHIASLSGALVAGLIFGGMAPLPAVLLSLAT
                     GLLFGFFSGLLVTKIGIPSLITTLATGIIAGGMVYMYTRGVSFYGQMPPSFLALGRGM
                     VGPVPSLIFIMFVVVAVAQVFISKTRTGKYMQATGANQAASRLAGINTDRCKILALSL
                     SGLAAAFTGILLTSRLGSANPEGASGFMMDA"
     gene            complement(735..2231)
                     /locus_tag="PK442_06155"
     CDS             complement(735..2231)
                     /locus_tag="PK442_06155"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO87267.1"
                     /translation="MSILETRNLTKEFPGVRALSDFSFTLEKGEIHGLVGENGAGKST
                     LVKILAGVYTPTSGSFLLGGREMRFRSPRDAAERVGVVHQERELIPHFSGWENLFLGM
                     EFSSLGFLRKRRMREEALRFVEKYSMNIDLDLPASELGSGRQEMLAILKVLFRDPEVV
                     IFDEPTAPLSVKECESLFALIRDLKAKGLPILYISHHLSEVLHLADRITVLRNGRKVS
                     TVHAKDADEQTLIRLMIERDMESQYPKRSVSAGEDVFTMERASSRKGRVRDVSIHVRR
                     GEIVGFAGLVGAGRTELAKAVFCGSRFEEGRMTLAGKPFSSRGPGESIRKGIAMIPEN
                     RREEGVVADMTVGENLVLPLLKQLASLGFLRSKQCDLHANSVVDKLAIKISGLSQETR
                     TLSGGNQQKVSVGKWFGTPSELWIFDEPTQGIDVDAKREVYTIMGDLAERGAGVWFIS
                     SDLRELLAIADRIYVMKDFRIAGEFLPPFDDEEILGSMMGEGLRNVAG"
     gene            complement(2228..>2959)
                     /locus_tag="PK442_06160"
     CDS             complement(2228..>2959)
                     /locus_tag="PK442_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOO87268.1"
                     /translation="PLYDGSAGMREIRDTAMRDAEALVEAGFDGIVFSNEGDRPYLSS
                     VDHVTVAAMSRVISETAATFDVPFGLSVLADPEAAVAIGAAVEADFVRIFLSWVFVGD
                     WGIVDPDAGKLQRLRASLAPSMKVFANISGHTEPLGGRKLEDIARGAVKFGLADAVCL
                     AGTTAGSEIPEEDLRGARRGAEGYPVIAGTGVCVENVERMLSLADGVIMGTSVKVDRD
                     TFKPVDPLKAAEFIRCAKDARDKMQ"
BASE COUNT          607 a          996 c          817 g          539 t
ORIGIN      
        1 acgcgtccat cataaacccg gaggctccct cggggttggc cgacccgaga cgggatgtga
       61 gcaagatgcc ggtgaaggcc gcggcaagac cggaaagtga gagcgccaaa atcttgcacc
      121 ggtccgtgtt gataccggca agccgggaag cggcctgatt cgcccccgtg gcctgcatgt
      181 acttccccgt gcgggtcttc gagatgaaca cctgcgcgac cgccaccacg acgaacatga
      241 tgaaaatgag ggaggggacc ggcccgacca tcccccgccc gagcgcgagg aacgaagggg
      301 gcatctgccc gtagaaagag acgccgcgcg tgtacatata caccatcccc ccggcgataa
      361 tgcctgtggc cagcgtggtg atgagcgacg gaatgccgat cttcgtcaca aggagccccg
      421 agaagaaacc aaagagaagt ccggtcgcca gagagagcag caccgcggga agcggcgcca
      481 ttcccccgaa gatcagcccc gccaccagcg cgccggaaag gctggcgatg tgcggaaagg
      541 ccaggtccag ctctcccacg atgaggcaca tcgtgaatcc ttccgagatc accgccaaaa
      601 gggcgatctg cctgagaata ttcattccgt tctcacccgc caaaaacccc ggcaccgcaa
      661 aagaaaaccc cgcgcacaac acgatcaggg cgagaatcgt cccgtaccgc gtcagcattt
      721 tttgcatttg aaatttaccc tgcgacattt cggagcccct cccccatcat gcttccaaga
      781 atttcttcat cgtcgaacgg agggaggaac tcccccgcaa tacgaaaatc cttcatgacg
      841 tatatccggt cggcgatggc aagcagctcg cgaagatccg agctgatgaa ccacacgccc
      901 gcccctcttt ccgcgagatc gcccatgatg gtgtagacct cgcgttttgc gtccacgtcg
      961 atcccctgcg tcggctcgtc gaaaatccag agctccgaag gggtcccgaa ccattttcca
     1021 acggagacct tctgctggtt tcctccgctg agcgtcctcg tctcttgcga gagaccggaa
     1081 atttttatgg cgagcttgtc cacgacgctg ttcgcatgca gatcgcactg cttcgatcga
     1141 agaaagccca gcgacgcgag ctgcttcaga agagggagca cgaggttctc ccccaccgtc
     1201 atatcggcga cgacgccctc ttcgcgcctg ttctccggga tcatcgcaat cccctttcgt
     1261 atgctctcgc ccggaccgcg ggaggaaaag ggtttccccg ccaacgtcat ccgcccctcc
     1321 tcgaagcgag agccgcaaaa gaccgctttc gccaattccg tgcggcctgc gccgacaagc
     1381 ccggcgaacc cgacgatctc gcctctgcga acatgtatcg aaacgtcgcg cactcttccc
     1441 ttccgggagg aggcccgttc catcgtgaac acgtcctccc ccgcggaaac gctccgtttg
     1501 gggtactggc tctccatatc ccgctcgatc atcaaacgga tcagcgtctg ttcgtccgca
     1561 tccttcgcgt gcaccgtgga aacctttctc ccgttccgga gaaccgtgat ccggtcggcg
     1621 aggtgcaaca cctccgaaag gtggtgggag atatacaaga tggggagccc tttcgccttc
     1681 aggtccctga tcagagcgaa gagactctcg cactccttca cgctcagcgg cgccgtgggt
     1741 tcgtcgaaaa tgacgacctc cggatcacgg aagagcacct tgaggatggc gagcatctcc
     1801 tggcggccgc tgccaagctc cgaagcggga agatcgaggt cgatgttcat cgaatacttc
     1861 tcgacgaaac gaagcgcctc ttcgcgcatt cgtcgtttcc gcagaaaacc gagcgaggag
     1921 aactccatcc cgagaaagag attctcccat ccggaaaagt gcgggatcag ttcccgctcc
     1981 tgatggacaa cgcccaccct ttccgcggcg tcgcgcggcg accggaaacg catctctctc
     2041 ccgccgagca ggaacgaccc ggaagtcggc gtatacacgc cggcgagtat cttcaccagg
     2101 gtggacttcc cggcgccgtt ctctcccaca agaccatgaa tctccccctt ctccagcgtg
     2161 aaggagaaat cggaaagcgc ccggactccg gggaattcct tggtgaggtt ccgggtctcg
     2221 agaatactca ttgcatctta tccctggcgt ccttcgcgca gcgtatgaac tccgccgctt
     2281 tcagcgggtc gaccggcttg aaggtgtccc tgtccacctt gacgctcgtc cccatgatga
     2341 cgccgtcggc aagggagagc attctttcga cgttctccac gcatactccc gtcccggcga
     2401 tcacgggata cccttccgct ccgcgcctgg cgccgcgcag atcctcctcg ggaatttcgc
     2461 tccccgccgt ggttcccgcg aggcagaccg cgtcggcgag tccgaacttg accgctcctc
     2521 tcgcgatatc ctccagtttc cgccctccca gaggctccgt atgccccgag atgttggcga
     2581 acactttcat cgagggagca agcgacgccc gcaggcgctg gagctttccc gcgtcggggt
     2641 ccacgatccc ccagtcgccc acgaaaaccc atgagaggaa gatgcgtacg aaatcggcct
     2701 ccacggccgc gcctatcgcc acggccgcct ccggatcggc cagtacggag aggccgaacg
     2761 gaacgtcgaa cgtggccgca gtctcggaaa tcaccctgct catcgccgcc accgtcacgt
     2821 gatccacgga ggagaggtac ggtctgtcgc cttcgttgct gaagacgatt ccgtcgaatc
     2881 ccgcttcgac gagcgcctcg gcgtcgcgca tggcggtatc gcgaatttcc ctcattcccg
     2941 cgcttccgtc atacagagg
//
LOCUS       DAOSWW010000256         2952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24882_length_2952_cov_2.974802, whole genome shotgun sequence.
ACCESSION   DAOSWW010000256 DAOSWW010000000
VERSION     DAOSWW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24882_length_2952_cov_2.974802"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PK442_06165"
     CDS             <1..777
                     /locus_tag="PK442_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO87269.1"
                     /translation="CVQLHAAAGKWLMEYIDSFRDENGTLHPLMEVKRTHSLRVRDNA
                     SKIAAELGWSGGLLDCALTAALLHDTGRFPQFAEWGTYYDGVSLDHGDLGEKKLREFF
                     PDEKAAIYGRWEDILLAVRLHNKKELPAGIAPAAAPLVRIVRDSDKLDVFRLVRMHVE
                     EDRINELLPRIIPSGGYSEALVREVSSERRASYSNVRSLSDFLLVQLSWVFDLNYAPS
                     FRMLRRDGVLSWMERVLPQDASVNGFIQSALHHALLGESA"
     gene            complement(845..2143)
                     /locus_tag="PK442_06170"
     CDS             complement(845..2143)
                     /locus_tag="PK442_06170"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOO87270.1"
                     /translation="MFTVKEFLKCEVKPALGCTEPGAVALAVARAKEELPGDVENVVV
                     TVSDSIYKNGVDVGIPGTRGLRGNNVAAALAVLCGRSEYGLEVLKDCAEEDAAKALEL
                     LGKGCIKVIPDMERHGVFVEAKIQSGGKTALCRIEGSHTNIVEVLLDDKIRFSTVCPE
                     STNISEQAKSISEEVSRMGFSELVSMVDSMDEEDIDYVLKGVEMNMHIAEYGFSPDHD
                     SGLNLGKTIRKHSGTGFDSDDLSLKIKSYAAAAADARMSGAPLPVMSSAGSGNHGVTA
                     ILPVAIFAAHLGADKEETAKAVAFSHLATSYVKSRTGRLTPTCGCTVAAGAGAAAGMT
                     YLKTKDIARAAQAMLIILGNLVGMVCDGAKYTCALKVGTGALEAYHAALLVFDGHYPE
                     PQGLVDETIEKTVNNMVQVSVHGMQNLDRAIIDVISHRFN"
     gene            2445..>2952
                     /locus_tag="PK442_06175"
     CDS             2445..>2952
                     /locus_tag="PK442_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOO87271.1"
                     /translation="MGVAISGPSLAGHVAKCGGVGTIASVGLAANSPHYNGRNYFDAN
                     QLAVKDAIADARKIAGPGGVIAVNCMVALTDYDRQTLSACQGGVDVIISGAGLPLRLP
                     EYTRDFPDVALVPIVSSLKAAALITRRWEKTYGRVPDGFVVETPKYAGGHLGVTKMDQ
                     VDDEQFTLE"
BASE COUNT          599 a          805 c          861 g          687 t
ORIGIN      
        1 tgcgtgcaac ttcacgccgc agcgggaaag tggttgatgg aatatatcga ttcattccga
       61 gatgagaacg gaaccctgca ccccctgatg gaggtgaaac gtacgcatag tcttcgagtt
      121 cgggacaacg cgtcgaaaat cgctgctgaa ctcggatggt ccggagggtt gcttgattgc
      181 gccctcacgg cggctctgct gcacgatacc gggcgctttc cccaatttgc cgaatgggga
      241 acctactatg acggcgtatc tctggatcac ggcgatctgg gggaaaagaa gctaagagag
      301 ttttttccgg atgagaaagc cgcaatatac gggcgctggg aggacatcct gcttgcggta
      361 cggctccata ataaaaagga gcttcccgca ggcattgctc ctgcggcggc acctttagtc
      421 cggattgtcc gcgacagcga caaactcgac gtctttcgcc ttgtccggat gcatgtggag
      481 gaggacagaa tcaacgagct gttgcccaga attattccct cgggaggcta ttcggaggcg
      541 ctggtacgtg aagtatcctc tgaaaggaga gcctcctaca gcaatgtccg ttccctctct
      601 gattttcttc tggtacagct ctcctgggtg ttcgacctta attatgctcc gtccttccgt
      661 atgctgcgga gagacggggt tctctcgtgg atggaacgcg ttctgccgca ggatgcgtct
      721 gtgaacggtt ttattcagtc ggctcttcac catgctttac tcggggagag cgcgtaaggc
      781 agaaaaagag ccaacgaagc ggcccggaga gcgagcgatc ttccgggccg cttcgtgacg
      841 tccgttaatt gaagcggtgt gagatgacgt cgatgattgc cctgtcgaga ttctgcatgc
      901 cgtggacgga aacctggacc atattgttca cagttttttc gatggtttcg tctacaagcc
      961 cttgaggctc ggggtagtga ccgtcgaaga caagcagcgc tgcgtgatac gcctccagcg
     1021 cgccggttcc tactttcagt gcgcaggtgt atttcgctcc gtcgcagacc atgccgacga
     1081 ggttgccgag gatgatcagc atggcctgag cggccctggc gatatctttt gtcttcaggt
     1141 aggtcatccc cgccgcagcg ccggcgccgg cggcgacagt gcagccgcag gtcggcgtca
     1201 ggcgtccggt gcggcttttt acatagcttg tggcgaggtg gctgaatgcg acagctttcg
     1261 cggtttcttc cttgtccgcg cccaggtgcg cggcgaagat cgccaccgga aggatggcgg
     1321 tgactccgtg gttgccgctg cctgcgctgc tcataacggg gaggggagct cccgacatgc
     1381 gggcgtccgc cgcagccgcg gcgtaggatt ttatcttcag gctgaggtcg tcggaatcga
     1441 atcctgttcc ggagtgcttt cgaatggttt taccgagatt gagtcccgaa tcgtggtcgg
     1501 gggagaaacc gtattccgcg atgtgcatat tcatctccac gcctttgagg acatagtcga
     1561 tatcctcttc gtccatggag tccaccatcg acacgagctc cgagaatccc atccgcgaca
     1621 cttcctcgga gatgctcttt gcctgttccg aaatgtttgt ggattcgggg catactgtgg
     1681 agaagcggat tttgtcgtcg agcagaactt ccacgatgtt ggtgtggctt ccttcgatgc
     1741 ggcagagagc ggttttcccg cccgattgga tttttgcttc gacaaagact ccgtgccgct
     1801 ccatgtcggg gattaccttg atacacccct tgcccagcag ctccaaagcc tttgctgcgt
     1861 cctcttctgc gcagtccttc agaacttcga ggccgtactc cgatcgtccg cagaggacgg
     1921 cgagcgctgc ggcaacattg ttccctcgaa gtcccctcgt ccccggaatg cccacgtcca
     1981 cgccgttttt gtagatgctg tcgcttacag tgacgacaac gttttccaca tctcccggca
     2041 gctcttcttt cgcccgggcg acagccaatg ccaccgctcc gggctcggta cacccgaggg
     2101 cgggttttac ttcgcacttc aaaaactcct taacagtgaa cattttgtcc cctccacgca
     2161 gtaaaatttg agaatcaggg tatatactag acaaatggta ccgtgcaacg aaaagcacgt
     2221 caatcgtttc ttggtgggga cagtgcgaaa cacgggaaag aagctatatt tttttcttgc
     2281 tcgtgttgtt cactataaga tacaatagaa gaatatgata gccctttgtt tgaagggcaa
     2341 gatccagaga acactttgca gaggagagaa tctttttgag tatacaactg cccgttttaa
     2401 agttaggaaa acatacgccc cggtatccct tgatacaggg cggcatggga gttgcgattt
     2461 caggacccag tctcgcgggg catgtggcga aatgcggcgg ggtgggaacg atcgccagcg
     2521 taggacttgc ggcaaactcc ccacactaca acggtcgcaa ctattttgac gcgaaccagc
     2581 tggcagtcaa agacgccata gcggatgcga ggaaaatcgc cggtcccggc ggagtgatcg
     2641 ccgtcaactg catggtggcg cttacggact acgaccggca gactctttcc gcctgccagg
     2701 ggggcgttga cgtcatcatc tccggagcgg ggcttcctct ccgcctgccc gagtacactc
     2761 gcgacttccc ggatgtggcg ctcgttccga tcgtcagctc gttgaaagcc gccgcgctga
     2821 tcacccgcag atgggagaag acgtacgggc gtgttcccga cggttttgtc gtcgagacgc
     2881 cgaagtacgc cggaggacat ctcggagtga cgaagatgga ccaggtggat gacgagcagt
     2941 ttacccttga ac
//
LOCUS       DAOSWW010000257         2947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_24949_length_2947_cov_4.484440, whole genome shotgun sequence.
ACCESSION   DAOSWW010000257 DAOSWW010000000
VERSION     DAOSWW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24949_length_2947_cov_4.484440"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PK442_06180"
     CDS             complement(<1..1140)
                     /locus_tag="PK442_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOO87272.1"
                     /translation="MEQMKLLTEWKYTPAKLNKGYTRQFLSVDLGKADGAFTCETHPI
                     SDEMVEKFTGGRGFGLRILWDKVTSKSKWNDPENAMVISGGPLSGTTQYPGMGKFYAV
                     FLSPLTQQTYNSNAGGYFGPFLKAAGFDALSVSGKAQKDVVVYIDGDEGAVRIFEWAP
                     ENRNSYTVTEALHEFFAENEEDKRNISVVSAGEAAEHSWWGAMNVSFYDPRRALPRLK
                     QAGRGGGGTVLRDKKILALVVKKKGFSGTENAPADLSTLQKVGTKLHREIRQNDDVQC
                     LMRKVGTAHLNLVMNDYDILPVNNFKFGRNEDIHNIDPDAYEKLFTQGYADGCWYGCS
                     MACAKAVDGFELTTGPDKGKKVTVDGPEYETASSLGSNVGIFDPLW"
     gene            complement(1145..1540)
                     /locus_tag="PK442_06185"
     CDS             complement(1145..1540)
                     /locus_tag="PK442_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO87273.1"
                     /translation="MKFLKPLLDKCVMCGACMSACSKAWAKQDNPAMSRIAVKNVTGL
                     PDIRVCDQCGGCIAVCPTMALQRDKNGVVQIDRKKCTSCLICVEFCPSASMFFHADID
                     SPFKCVACGICVKACPEGALSIVEQKQEG"
     gene            complement(1576..2724)
                     /locus_tag="PK442_06190"
     CDS             complement(1576..2724)
                     /locus_tag="PK442_06190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOO87274.1"
                     /translation="MEWKSTAEVVIVGGGIIGVSTAYYLAKQGVKDIVLVEKGTICGG
                     STGRCGAGIRAQWGTEMNCRLGIAALDIFEQLDQELGMSVGLNQGGYLLVAYNEKELE
                     TLRNGMLLQNSLGIQSRMVTLTEAREICPGLAAEDAVGFFFHGRDGHADPFLTTFAYL
                     EAAKRLGVTCYRNTACTGVIVRDNTVQGIETTNGKISAPIVINCAGAYAQIVARMAGI
                     ELPNWAERHEIMITEPVAPGVCPCMLMSFSGNYYIQQRPHGSIICGMSPQGHPEDFEN
                     KTTWQFIEAMSKTLVKLLPRTKGIRIARQWSGLYDMTPDAQPVIGETDVKNFYHSTGY
                     SGHGFMLAPVAGKILAQHITGQKPDIDFSMLDYRRFSRGEHIRESNVV"
     gene            complement(2724..2945)
                     /locus_tag="PK442_06195"
     CDS             complement(2724..2945)
                     /locus_tag="PK442_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO87275.1"
                     /translation="MDEWIDRGYDTVEELKRVLRVGMGPCQGRGCQDILMREIAKKTG
                     KPISEVAPATVRPPVKPIKIGLLAEGGEE"
BASE COUNT          616 a          826 c          754 g          751 t
ORIGIN      
        1 ccacaacggg tcaaaaatac caacattaga gccgagactt gacgctgttt catattccgg
       61 gccatctacg gtgacttttt tccctttatc gggtccggtc gttagctcaa agccgtctac
      121 agcctttgca caggccattg aacagccgta ccagcaaccg tcagcgtatc cttgagtgaa
      181 aagtttttcg taggcgtcag gatcaatatt gtgaatgtcc tcgtttcttc cgaatttgaa
      241 gttgttcacc ggaagaatgt cgtagtcgtt cataacgagg ttaaggtgcg ctgtgcccac
      301 ttttctcatg agacactgta cgtcgtcgtt ctggcgaatt tcacggtgga gttttgtccc
      361 gactttctgc aaagtggaca gatcagccgg ggcgttttcg gtaccagaaa agcccttctt
      421 tttgacgacg agcgcgagga tctttttgtc cctgagcaca gtgccgccgc cgccgcgtcc
      481 agcttgcttc aagcgaggaa gcgctctcct tgggtcatag aaactgacgt tcatagcccc
      541 ccaccagctg tgttccgcag cttctccagc cgagacaacc gagatgttgc gcttgtcttc
      601 ttcgttctcc gcgaagaact catgaagcgc ttccgtgacg gtataggagt tacggttctc
      661 aggcgcccat tcgaagatac gtaccgcgcc ttcatcgccg tcgatataca ctacaacgtc
      721 tttctgcgct ttgccagata cagaaagggc gtcaaacccg gcagccttca ggaatggccc
      781 gaagtaccct cctgcattgc tgttgtacgt ctgttgcgta agcggcgaaa ggaataccgc
      841 gtaaaacttg cccatgcccg gatattgcgt ggtgccggaa agcggccctc cggagatgac
      901 catcgcgttt tccgggtcgt tccacttgct ctttgaagtg accttgtccc agaggatacg
      961 aaggccgaaa ccgcgtccgc cggtaaactt ctccaccatc tcgtcggaaa tagggtgtgt
     1021 ttcacaggtg aacgcgccgt cggccttgcc gagatccaca gaaaggaact ggcgtgtata
     1081 ccctttgtta agtttcgcag gagtgtattt ccactcggtg agaagcttca tctgttccat
     1141 tctcctatcc ctcctgtttt tgctcgacta tagacagagc gccctcgggg cacgccttta
     1201 cacaaattcc acaggcgacg cacttgaacg gagagtctat gtccgcatgg aagaacatgg
     1261 aggcggaggg acaaaactcg acgcaaatga ggcaggatgt gcactttttc cgatctatct
     1321 ggacgactcc attcttgtcc ctctgcagcg ccattgttgg acagacggcg atgcagccgc
     1381 cgcactgatc gcataccctg atgtcgggca gccctgtcac gttcttgacg gcgatgcgcg
     1441 acatcgcggg attgtcctgc ttggcccacg ccttcgagca tgcagacata catgctccgc
     1501 acatgacgca cttgtcgagt aaaggtttca gaaacttcac aacaaacact ccttttctgg
     1561 tgatctatct ttccatcata ccacgtttga ttctctgatg tgctctccgc ggctgaagcg
     1621 acgataatcg agcatcgaaa aatcgatgtc cggtttttgt cctgtgatat gctgggcgag
     1681 aattttcccg gctacggggg cgagcatgaa tccgtgtccg gaatatcccg tagagtgata
     1741 gaaattcttg acgtcggtct ctccgatcac cggctgcgca tctggagtca tatcgtacag
     1801 tcctgaccac tgccgcgcta tccttatgcc ttttgttctc ggaaggagct tgacgagcgt
     1861 tttcgacatt gcttcgatga actgccatgt cgttttgttc tcgaaatcct cggggtgccc
     1921 ttggggactc ataccgcaga tgatggagcc atgcggacgc tgctgaatgt aatagtttcc
     1981 ggagaaactc atcagcatgc aggggcagac tcccggagcg accggttcag tgatcattat
     2041 ctcatggcgc tcggcccagt tgggaagctc gatacctgcc atacgggcga ctatctgagc
     2101 atatgctccg gcgcagttga tgacgatcgg ggcgctaatt tttccgttag tggtttcgat
     2161 cccctggaca gtgttgtccc ggacgatgac tccagtgcat gcagtgtttc tatagcatgt
     2221 tacgccgagg cgcttggcag cttcaaggta agcgaatgtc gtaaggaacg ggtcggcatg
     2281 cccgtctctg ccgtggaaaa agaaaccgac cgcgtcttcc gctgctaggc ccggacagat
     2341 ttcgcgggct tcggtgagag tgaccatgcg cgactgtatt ccaagtgagt tctggaggag
     2401 catcccatta cggagcgttt ccagctcctt ttcgttatac gcaacaagga gataccctcc
     2461 ctgattcaat ccgacggaca tcccaagttc ctggtcgagc tgctcgaata tgtccagagc
     2521 ggcaataccc agacggcagt tcatttcggt tccccactgc gcacggattc ctgcgccgca
     2581 acgtcccgta gaacctccgc agatagttcc cttttcaacg aggacgatgt ctttaacccc
     2641 ttgtttcgca agatagtagg cggtggaaac tccgatgata cctccgccta cgattacaac
     2701 ctcagcggtt gatttccact ccatcactcc tcacctcctt ctgcaaggag ccctatctta
     2761 ataggtttta cgggggggcg aaccgttgcc ggggctactt ctgaaatggg ctttcctgtt
     2821 tttttcgcga tctccctcat gagtatatcc tggcacccgc gtccctggca tgggcccatt
     2881 ccgactcgaa gaacgcgttt caattcctcg acggtgtcgt atccgcgatc aatccactcg
     2941 tcgatct
//
LOCUS       DAOSWW010000258         2942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25011_length_2942_cov_3.434361, whole genome shotgun sequence.
ACCESSION   DAOSWW010000258 DAOSWW010000000
VERSION     DAOSWW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25011_length_2942_cov_3.434361"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PK442_06200"
     CDS             <1..1186
                     /locus_tag="PK442_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO87276.1"
                     /translation="DANKKKAAFIANLSHEIRTPLNSIVGVVNLFQGTKLDLEQKKFV
                     EMLSSSSRDLLLLVEELLDLSRLETGQLELKSDPVPLRSCCSQAVRPLAMTAQERRLQ
                     MELSIAPDIPEFLCGDSARLRQVLRNIVGTAIAFTAKGKIDIRVSIVHETRETITLQF
                     SVAHSAEGIAGERINAMLDSSALDRSEFSRAYEGVGLGLIIAKGLAEAMGGGLRAESV
                     GNEHNIFHFTVAMKRTAENKQTSEEPAAQPEQETPDALLALPGRLAILVVDDNKFNRS
                     LTRTILRKKGGPEWDISLAEDGAEALKKISGEHFDLVFMDIQMPGINGLDTTRAIRAR
                     ERETVAQRLPVIAMTAYAMEGDRQMCLDAGMDDYIAKPVDPDELVEVIIRNLRMITPD
                     ER"
     gene            complement(1213..2274)
                     /locus_tag="PK442_06205"
     CDS             complement(1213..2274)
                     /locus_tag="PK442_06205"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO87277.1"
                     /translation="MKNALQTSSPFPLALEVAGFRFNGMNLLDMFGESLVMWETATGH
                     IVYMNEAARVLYDYTLAETAALSIEEIFPSRDEVADRSFSVITTSHRKKSGIFFPVQA
                     TCRTLAQDTEEIMMMVVADISPENLARDDVALASDIQRGFLPGDLVGEPDVEVRSIYR
                     PMFLISGDMFGYHWDPSARVLNGYIYDLMGHGVPAALQTSALMVLFRQAFEDEDRIGS
                     SLTEKLQWVNDTAAERFLSDSFAAAICFRLDLRKKKLSYCAAGINAFLHGVNGELKKV
                     PTPGSLLGLSSRVEFGSGEVSVKPGDYFLFTSDGFLDLLEGTETLRMEFPDAYDHLFQ
                     IGYTGASRDDLSALCVHIR"
     gene            complement(2284..2610)
                     /locus_tag="PK442_06210"
     CDS             complement(2284..2610)
                     /locus_tag="PK442_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87278.1"
                     /translation="MRLFVAVNEAVNNALFHANRDLPEACVELVAVDEGASLRISVRS
                     EGDGFEHDLRADPGVFSDFLRESGRGVQIILHVVDEVSIEESGSIVTLRMGKDDSQRR
                     NSSIPS"
BASE COUNT          703 a          869 c          791 g          579 t
ORIGIN      
        1 ggacgcgaac aagaaaaaag ccgcgttcat cgcaaatctg agccatgaga tacgaacgcc
       61 cctcaattca atcgttggag ttgtcaacct gtttcaaggg acgaaactcg acctcgaaca
      121 aaaaaaattc gttgaaatgc tctcgtcatc ctccagggat ttactcctgc tcgtcgagga
      181 gctgcttgat ctttcgcgcc tcgaaacagg gcagcttgaa ctaaaatcgg atcccgtacc
      241 tcttcgatcc tgctgctcgc aggcggtacg ccccctggca atgacggccc aagagcggcg
      301 tcttcagatg gaactctcga tagcgcccga tattcccgaa tttctctgcg gagacagcgc
      361 ccgactgcgg caggtactcc gaaatatcgt cggtaccgca atcgcgttta ccgcgaaagg
      421 caaaatcgac atcagggttt ccatcgtcca cgaaacccgc gaaacgatta ctctccagtt
      481 ttctgttgcc cactcggcgg agggaatcgc cggcgagaga atcaacgcga tgctcgactc
      541 ttcggccctg gaccgcagcg aattctcccg cgcctacgaa ggggtgggtc ttggactgat
      601 catcgccaag ggattggccg aggccatggg gggcgggctc cgggcagaga gtgtcgggaa
      661 cgagcataat attttccatt tcacagtagc aatgaaaagg actgccgaga acaaacagac
      721 cagcgaggaa ccggcagcac agccggagca ggaaaccccg gacgcgctcc tcgccctgcc
      781 ggggaggctc gccatcctcg tcgtcgacga taataagttc aaccgcagcc tcacccggac
      841 aatcctgcga aaaaagggcg gcccggaatg ggacatttcg ctggcggaag atggcgccga
      901 agcgttgaaa aaaatttccg gcgagcattt cgacctggtc ttcatggaca tccagatgcc
      961 cgggataaac ggcctggata caaccagggc catacgggca cgcgaaaggg aaactgtagc
     1021 gcagcgtctt ccggtcatcg ccatgaccgc ctacgccatg gagggagaca gacagatgtg
     1081 ccttgacgcc gggatggacg actatatcgc gaagccggtc gaccctgacg aactggtgga
     1141 ggtcatcatc cgcaacctcc gcatgattac tccggacgag cgataacctt gtgagccttg
     1201 ctgtgcgccg gcttagcgga tatggacgca gagggcggaa agatcgtccc tgctggctcc
     1261 tgtatagcca atttggaaga gatggtcgta ggcatccgga aattccatcc gcagcgtttc
     1321 cgttccctcc agcagatcaa ggaatccgtc gcttgtgaat aaaaagtagt cccccggttt
     1381 cacagagacc tctcccgatc cgaactctac ccggctggaa agcccgagga gcgaccccgg
     1441 agtgggcacc tttttcaact ccccgttcac tccgtgcagg aacgcgttga ttcccgccgc
     1501 gcagtaggag agtttcttct tgcgcaggtc aaggcggaaa cagatcgccg cggcgaacga
     1561 atccgaaagg aagcgctcgg cagccgtatc gttcacccat tgcagcttct ccgtcaggct
     1621 ggagccgatt ctgtcctcgt cctcgaacgc ctgccggaag aggaccatca gggccgacgt
     1681 ctggagcgca gcgggaacgc cgtgtcccat gaggtcgtag atgtagccgt tcaaaacccg
     1741 tgcggaagga tcccaatgat agccaaacat atccccgctg atgagaaaca tcggccggta
     1801 gatgctccgc acttcgacat cgggctctcc cacaagatct ccgggaagaa atccccgctg
     1861 aatgtccgag gcgagcgcaa cgtcgtcccg cgccagattt tccggcgaga tgtcggcgac
     1921 aaccatcatc atgatttctt cggtgtcctg cgccaatgtg cggcacgttg cctgcacggg
     1981 aaagaagatg ccgctcttct ttctgtgcga ggtcgtaatc acggagaacg acctgtcggc
     2041 aacttcatcg cgggaaggaa aaatttcctc gatggaaagg gccgcggtct ccgccagcgt
     2101 gtagtcgtag agcaccctcg ccgcctcgtt catgtacacg atatgccccg tcgccgtctc
     2161 ccacatcacg agagattctc cgaacatatc cagcagattc attccgttaa aacgaaaacc
     2221 ggcaacttcc agagccaagg ggaagggcga agatgtctgg agagcgtttt tcatgcgatc
     2281 acctcatgac ggaatcgagg agtttctcct ctgcgaatca tcttttccca tacgaagcgt
     2341 gacgatactt cccgactctt caatgctcac ctcgtcaacg acgtggagaa taatttgcac
     2401 tccccggccc gactccctga gaaaatcgga aaagacgccg ggatcagcac gcaagtcgtg
     2461 ttcaaaaccg tcgccttcgc tccgtacgga tatacgtaag ctcgcgccct catcgacggc
     2521 aacaagctcc acgcacgctt caggaaggtc cctgtttgcg tgaaaaagcg cattgttgac
     2581 cgcctcgttt accgctacaa aaagacgcat gcctctctgc gaaccgcaca ttgagcacat
     2641 aagcgttaaa agaccgtttc tttgaggggc gaattcctct attgtggaga atacaatcga
     2701 tatcaagttc tcctctctgc acataccatc actcctcgcc tccggaaata ggggacgccg
     2761 ttcgtcttct tacggccata atagcacaga gaaagagcga ggaaaagaaa aaggaggccg
     2821 catatcgcag cctcccgctc tggagaaaac ctaaatcggc gctatttcag cgctttggcc
     2881 agagtttcgg cccggtccgt ccgctcccat gcgggcagcg gatcgaggtc gatacggccg
     2941 at
//
LOCUS       DAOSWW010000259         2939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25051_length_2939_cov_2.939667, whole genome shotgun sequence.
ACCESSION   DAOSWW010000259 DAOSWW010000000
VERSION     DAOSWW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25051_length_2939_cov_2.939667"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..2532
                     /locus_tag="PK442_06215"
     CDS             214..2532
                     /locus_tag="PK442_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87279.1"
                     /translation="MTLKIEEHILQSPLIGFAHHRILLDDFGKPVDYEYLAVNATFEK
                     LTGLDAASLLHRTIRQVVPGFEKTGSDWIRIFGEVALGGGEREFEQFSEPLGKWFRVH
                     VYSAERLYFTTLFLDVTESKKQAEELEGFFRVNLDLLCIADIDGNFVKTNAAWGEILG
                     YSSEELDGNRFLDFVHPDDLPATLDAMSALGEGTKVMNFVNRYRCKDGSYRFIEWRSH
                     PKGRLIYAAARDITEHRLIEDELRAATMRAEEMARKAKAASIAKSEFLANMSHEIRTP
                     MNGVLGMTELLLAGALSEEQRQYAQTIRASAESLLDILNDILDISKVEAGKLELKTLD
                     FNLDMLREEFESSMRAVAEHKGLSLFCSVDPDVPRCLRGDPGRLRQIMTNLTGNAVKF
                     THRGEVRTNASVVEQDDRSVLLRFTVRDTGIGIPADKLGTLFEKFTQVDASSTRQFSG
                     TGLGLAISRQFAELMGGSIGVTSEEGKGSEFWFTVRLEKQPEGACRDEVHRHAAATAP
                     RVERRGVRVLLAEDNAVNRKVALGMLKKLGFTADSVADGAQVLSALSERTYDLVLMDC
                     QMPVMDGYEATRRIRALEKPGGKRIPVVAMTAYAMSGDRERCIEAGMDDYLAKPISIE
                     ALSAALASWLPHREGPGGTDGSPRRSEPPTPRKETETCSKSAVWRKDELLNRLLGDEE
                     MTREILEAFLEDIPREIDSLAAAVAVGNTPTVELLAHTIKGAAGNVGSEGLRSAAFKM
                     ESAAKKGDLSGAQSLLKSIRQEFEHLKAAIRG"
     gene            complement(2649..>2939)
                     /locus_tag="PK442_06220"
     CDS             complement(2649..>2939)
                     /locus_tag="PK442_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87280.1"
                     /translation="RVRLKLRLESRLESRLESRLAAKVVLLIESQEAGKAELARELGH
                     KSVSGELHKQIRRLIALDWIEMTVPEKPNSRLQKYRLTDEGKKALDIEKEQK"
BASE COUNT          630 a          893 c          900 g          516 t
ORIGIN      
        1 atggaaagag gttcacagat ctctcgtgaa aacttgctgc ggcgctcgta cgtctttcgc
       61 agggttggtt ggcgttgaaa agcccgcccg gagaagcggt atgatatgat tgtatcgagt
      121 ataaatcgac aaagtaatca tgataaagaa cggtgcatcg cccggacagt cccggtgctg
      181 ctgattcaca cacacaacgg aggcgcttcc agcatgacgt tgaaaataga agaacatata
      241 ctgcagagcc ccctgatcgg cttcgcccat caccgcatcc tgctcgacga cttcgggaag
      301 ccggtggact acgagtatct cgcggtgaac gccaccttcg agaagctgac cggactcgac
      361 gcggccagtc tgctgcaccg cacgatacgt caggtcgttc cggggttcga gaaaaccggg
      421 tccgactgga tccgcatttt cggcgaggtc gccctcggcg gcggagaacg cgagttcgag
      481 cagttctccg agcccctcgg caagtggttc cgcgtccacg tctactccgc cgagcggctc
      541 tacttcacga cgctcttcct cgacgtcacc gagagcaaga aacaggcgga ggagctggag
      601 ggcttctttc gagtgaatct cgacctgctc tgcatcgccg atatcgacgg caacttcgtg
      661 aagaccaacg ccgcgtgggg ggagatactg gggtactcat ccgaagagct ggacgggaac
      721 agatttctcg acttcgtcca tccggacgac ctcccggcga cgctggacgc catgagcgcg
      781 ctcggcgaag gtacgaaagt gatgaacttc gtcaaccgct accgctgcaa ggacggctcg
      841 taccgcttca tcgaatggcg ttctcacccc aaaggcaggc tgatctacgc cgctgcgcgc
      901 gacatcaccg aacacaggct catagaggac gagcttcgcg cggcgacaat gcgggcggag
      961 gagatggcgc ggaaggccaa ggcggccagc atcgccaaga gcgagttttt agccaacatg
     1021 agccatgaaa tccgcacgcc catgaacggc gtgctcggaa tgacggagct gctgcttgcc
     1081 ggcgctctca gcgaggaaca gcgacagtac gcccagacga tcagagcaag cgccgagtct
     1141 ctgctggaca tcctcaacga catcctcgac atctcgaagg tggaagccgg aaagctggag
     1201 ctcaaaacgc tggacttcaa cctcgacatg ctcagggaag aattcgagtc ctccatgcgc
     1261 gcggtcgcgg aacacaaagg gctctccctc ttctgcagcg tcgaccccga cgttccgcgc
     1321 tgcctgaggg gcgacccggg ccgattacgg cagatcatga cgaacctgac ggggaacgcc
     1381 gtgaagttca cgcatcgcgg agaggtacga accaacgcct ccgtagtcga acaggacgac
     1441 cgctccgtcc tcctccgctt cacggtgcgc gacaccggca tcgggattcc ggccgacaaa
     1501 cttggaacgc tcttcgagaa gttcacgcag gtggacgcct cctccacccg ccagttcagc
     1561 ggcacgggac tcggccttgc gataagcagg cagttcgccg agctgatggg cggctccatc
     1621 ggggtaacga gcgaagaggg gaaggggtcg gagttctggt tcacggtccg tttggagaag
     1681 cagccggaag gcgcctgccg cgacgaggtc caccgccatg ccgccgcgac cgccccgcgc
     1741 gtggagagaa ggggcgtgcg cgtcctgctc gcggaggaca acgccgtgaa cagaaaagtc
     1801 gcccttggga tgctgaaaaa gctcgggttc acagccgact ccgtcgcgga cggggcacag
     1861 gtcctctccg cgctctcgga acgcacgtac gatcttgtgc tcatggactg tcagatgccc
     1921 gtgatggacg gctacgaggc aacccgtcgc atccgcgctc tggagaagcc cggcggaaag
     1981 cgaataccgg ttgtggcgat gaccgcgtac gcgatgagcg gcgaccggga acgctgcatc
     2041 gaggcgggaa tggacgacta tctcgcaaag ccgatctcga tcgaggcgct gtcggccgcc
     2101 cttgcgtctt ggttgccgca cagggaagga cccggcggaa cggacgggag cccccgccga
     2161 tccgagccgc cgacgcctcg gaaagaaacg gaaacgtgct cgaaaagcgc cgtctggaga
     2221 aaggacgaac tgctgaacag acttctggga gacgaggaga tgacccgcga aatcctggag
     2281 gcgtttcttg aggatattcc ccgggagata gattcgctcg ccgccgccgt ggctgtgggc
     2341 aacacaccca ccgtggagct gctcgcccac accatcaagg gcgcggcggg gaacgtggga
     2401 agcgaggggc tgcgctcggc ggccttcaaa atggagtcgg ccgccaagaa gggcgatctg
     2461 tcaggagcgc agtcgctcct gaagagcata cgacaggagt tcgagcacct gaaagcggcg
     2521 attcgaggat aaacgcgcgc cccgctacgc caaaccggcc ggttcgtaga cgggagagaa
     2581 aagacccgga tggcctgccg cacggcgtcg cgggtggtct tcttgtcctt cggatgaatc
     2641 gaactgtttc acttttgttc tttctctata tccagcgcct tttttccttc atcggtcagg
     2701 cgatacttct gcaaccgact gttcggcttt tccgggacgg tcatttcgat ccagtcaaga
     2761 gcaatcaacc gcctgatctg cttgtgaagc tcacccgaga cgctcttatg acctagttcc
     2821 cgcgctaatt ccgcctttcc tgcttcctga gattcgatca acaagacgac tttagccgcc
     2881 agccgcgact ctagccgcga ctctagccgt gactctagcc gcaactttaa cctgacccg
//
LOCUS       DAOSWW010000260         2931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25146_length_2931_cov_4.266342, whole genome shotgun sequence.
ACCESSION   DAOSWW010000260 DAOSWW010000000
VERSION     DAOSWW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25146_length_2931_cov_4.266342"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="PK442_06225"
     CDS             <1..1486
                     /locus_tag="PK442_06225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO87281.1"
                     /translation="RESVKRVQGLIPLKAAVIYLVGDDASFYPAYCSPPDALPELEKR
                     VATLIEDRTFVWAIKRRKPVLTEFKEKRAPLLLHVLSTISRTRGMLVGVMDKGWEEIS
                     DYSLSLLSVALTASAGALESFELYRNLRSLNRDLSSKMAQLEDSRNELERYHVVLEEQ
                     VETRTRELAQANDEMKMEIAERMRAEEELRSSESMLKHALEGVGEGIWMWDVASGEVD
                     LSPLSMEILGCAADVAACPPEMRWMEGWERLIHPDDVEKVRYKKQKCLAGDTQGYRVE
                     YRVPGGDGAVRWILERARVVLRDDARKPLRIAGTHSDVTERRELEDQIRFQATHDYLT
                     GLPNRYLFYDRFSQAIAYARRKKRKVSLLFIDLDNFKVVNDTYGHDVGDDLLKEAAER
                     LRKVFREMDTICRLGGDEFTVILPETGDRAEVQRIAERLLEFFDTPFPMNSGSIRLQI
                     SVGISIFPDDGDDIEMLLKKADLAMYRTKKHQRSAFAFAADVHD"
     gene            1648..2931
                     /gene="larA"
                     /locus_tag="PK442_06230"
     CDS             1648..2931
                     /gene="larA"
                     /locus_tag="PK442_06230"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOO87282.1"
                     /translation="MRVSIPYGTSALSCDIPDSRLKAVLVSSLHELKADAPERQIVRK
                     SLENPIGSLGLRELAEGKKNVVIISSDHTRPVPSAIIMPLLLEEVRAGNPDAEITILV
                     STGGHRATTPGELLKKYGTEIVKNERIVVHDSHDDASLVHVGTLPSGGDLILNKLAVE
                     ADLLVAEGFIEPHFFAGFSGGRKSVLPGVASYTTVMANHCAEFIAHERARTGILEGNP
                     IHGDMVFAARKVGLAFVCNVVIDAEKHIAAAFSGDMEAAHEEGCAFVGKYGRVKAVPA
                     DIVVTSNAGYPLDQNLYQAVKSMTAGEASCTKGGVIIVAAECSDGHGGEAFYRTFETI
                     PTPKGIMDMIMARGRNQTEPDQWQIQIFARVLMDFTVIMVTSAPKEMAEHLNMQWAPS
                     LEEALSMAERILGKPDASVTVIPDGVAVIVDAFSS"
BASE COUNT          641 a          812 c          906 g          572 t
ORIGIN      
        1 ccgggagtct gtcaagcgag tgcaggggct tatccctctg aaagcggccg tgatatatct
       61 cgtcggcgac gacgcttcat tctaccccgc gtactgctcc ccgcccgatg cgcttcccga
      121 gctggagaag cgtgttgcta ctctcatcga ggacagaacg ttcgtctggg cgatcaagag
      181 gcgcaaacct gtcctcaccg aattcaagga aaagcgcgct ccgctgctgc tccacgtgct
      241 ctccaccatc tccaggacgc ggggcatgct ggtaggggtc atggacaagg ggtgggagga
      301 gatctcggac tactctctct ctcttctctc cgtcgctttg actgcaagcg cgggggctct
      361 ggagagcttt gaactgtacc ggaatcttcg cagtctcaac agagatctct cctcgaagat
      421 ggcgcagctg gaggactcgc ggaacgagct tgaacggtat cacgtggttc tggaggaaca
      481 ggtggagact cgtaccagag agctggcgca ggcgaacgac gagatgaaga tggagatcgc
      541 cgaacggatg cgcgccgagg aagagcttcg ttccagcgag agtatgctca aacacgccct
      601 tgagggtgtc ggcgaaggaa tatggatgtg ggacgtcgct tcgggagagg tcgatctttc
      661 gccgctttcg atggagattc tcggatgcgc cgccgacgtc gccgcctgcc cgccggagat
      721 gcggtggatg gaggggtggg aacggctcat tcacccggat gatgtggaga aggtccggta
      781 caagaagcag aagtgccttg ccggagatac gcaggggtac agagtcgagt accgcgtgcc
      841 gggcggcgac ggggctgtca ggtggatttt ggagcgcgcc cgcgttgttc tcagggacga
      901 cgcgagaaaa ccgcttcgga tagccggcac ccactccgat gtcaccgaac ggcgggagct
      961 ggaggaccag atccgcttcc aggcgacgca cgactatctg accgggctgc cgaaccgcta
     1021 tcttttttat gatagattct cccaggcgat tgcgtatgca cgccgaaaaa agagaaaggt
     1081 ctcgctgctc tttattgacc tggacaattt taaggtcgtc aacgatacgt acggtcatga
     1141 tgtcggcgac gacctgctga aggaagccgc agaacgtctg agaaaggtct tccgggagat
     1201 ggataccatc tgccgccttg gcggtgacga attcacagtg attcttcccg agacggggga
     1261 ccgcgctgaa gtgcagcgca ttgcggagcg gctcctggag ttcttcgata ctcccttccc
     1321 catgaattca ggttcgatac ggctccagat atccgtgggg atcagcatct tcccagatga
     1381 cggcgacgat atcgagatgc tcctgaaaaa ggcggacctc gccatgtacc gcacgaagaa
     1441 acaccaaaga agcgcgtttg cctttgcggc agacgtgcat gattgaccag cctttcaccg
     1501 gaaactcaaa aaggaaaacg ccctaataaa gtcaattctt gcatgcgcct cttgacctct
     1561 tgttcaattg tcttaataat gtttattgga attttcaaaa agtagcgtgc ggcagcagaa
     1621 tgatttttca acaggaggcg agtgcacatg cgagtatcca ttccgtacgg aacgtcggct
     1681 ctttcctgcg atatacccga cagccgtttg aaagcggttc tcgtttcgag tcttcacgaa
     1741 ctgaaagccg atgcgccgga gcgccaaatc gtcagaaaat ccctcgaaaa cccgatcgga
     1801 tccctcggcc tgcgagagct cgcggaggga aaaaagaacg tcgtgattat ctcaagcgac
     1861 catacccgcc ccgttccaag cgccattatc atgccgctgc tcctcgaaga ggtgcgggcg
     1921 gggaaccccg atgccgaaat caccatcctt gtctctaccg gaggacaccg cgccaccacc
     1981 ccaggggagc tcctgaagaa gtatgggacc gagattgtga agaacgagcg cattgttgtc
     2041 cacgacagcc atgacgatgc gagtctcgtt cacgtaggaa ccttgccttc cggaggcgat
     2101 ttgatactca acaagctggc ggtcgaggcc gacctgctcg tcgcggaggg gttcatcgag
     2161 ccgcacttct tcgccggttt ctcgggaggc cgcaagagcg tgcttcccgg cgtggcaagc
     2221 tacacgacgg tgatggcgaa tcactgcgcg gagtttattg cgcacgaaag ggccagaacc
     2281 ggaattctcg agggcaaccc catccacggc gacatggtct tcgccgcccg gaaggtcggg
     2341 ctggcgttcg tctgcaacgt cgtcatcgac gcggagaagc atatcgcggc ggccttcagc
     2401 ggcgacatgg aagcggcgca cgaggaaggg tgcgcgttcg tcggcaagta cggcagggtc
     2461 aaggccgttc ccgccgacat tgtcgtcacg agcaacgcgg ggtatcccct ggatcagaac
     2521 ctctatcagg cggtgaagag catgaccgcg ggggaggcgt cgtgcaccaa ggggggcgtc
     2581 atcatcgtcg ccgccgaatg cagcgacggt cacggcggcg aagccttcta ccggaccttc
     2641 gagacgattc cgacgccgaa ggggatcatg gatatgatca tggcgagggg aagaaatcag
     2701 acggagccgg atcagtggca gatccagata ttcgccagag tgctgatgga tttcaccgtg
     2761 atcatggtga cctcggcccc caaggagatg gcggagcatc tcaacatgca gtgggccccg
     2821 tccctggagg aggccttgtc gatggcggag cgaatcctcg gaaaaccgga cgcatcggtc
     2881 accgtcatcc cggacggcgt ggcagtgatc gtcgatgcgt tctcttcctg a
//
LOCUS       DAOSWW010000261         2920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25292_length_2920_cov_3.537522, whole genome shotgun sequence.
ACCESSION   DAOSWW010000261 DAOSWW010000000
VERSION     DAOSWW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25292_length_2920_cov_3.537522"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2605
                     /locus_tag="PK442_06235"
     CDS             <1..2605
                     /locus_tag="PK442_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87283.1"
                     /translation="GRSESFEMEYPCHSPDEKRWFLMRVTPLLFPEGGAVVAHIDVTK
                     EKVATQTVKESEQRLLAVLNSIDALVFVTDVDTFEILMINDFGRKIFGDVVGRRCWEA
                     IQKGQTGPCEFCAEAPLLGDDGEPAGVRRWQVFNERTKRWYDCRDSAIRWTDRKYVRL
                     EIAMDITETKKIEEELRVHRDELDRLVAVRTEELSESNRELERQTEFAQEMALKAEAA
                     NEAKSAFLANVSHEIRTPINAVIGFTELLLDSTLSSEQRRQAETVRASAETLLFLINE
                     ILDFAKIEAGKLELEVVNFDLRNTVEGFAGTLALQAYTKGIELACGVEPSLPSKVRGD
                     PVRIRQILMNLGSNAVKFTQEGETVISVSKESETEETLWIRFSVRDTGIGIPGEKMGL
                     LFTKFTQLDASTTRQYGGTGLGLAISKHLVERMGGSIGVESEHGNGSTFWFVLPFEKN
                     GRGAEAPEAPEGVAGLRILVVDGNAASQAFFLRQFESWGMRAVVSSRTEALDVLRRGF
                     SDEDPFRVVLDADSGRSGEHSLGDAIMNDESLSETVLITLVPLGIRHGINPPDGERRY
                     SLVKPVQSAELAAALTAIALGGTVFSVAHEDGKESSSWLLSCGENARILLVEDNAVNR
                     QVALALLKKAGVRADTAENGLEALEALRSNDYDLVLMDLQMPVMDGLAATRAIRAGRG
                     DVRNPDVPVVAMTAHALQSDREACMHAGMNGYITKPIRPAELLCELERRLCAETAPAR
                     DNRREYVTRTGPRTFNFDDLVERLGDEETAGLIMEAFLKEMPEAMSALEKSLRTGDFL
                     LSGRNAHTIKGVAGNIGGEALREAALQFERAAKDGDTKLLHALRERVHAEYCALKDEI
                     ERMLRTLRPPE"
BASE COUNT          647 a          742 c          925 g          606 t
ORIGIN      
        1 cggccgttcc gaatcgttcg agatggagta tccttgccac tcgcccgacg agaaacgttg
       61 gttcctgatg cgcgtcacgc cgcttctctt ccccgaaggg ggggctgtgg tagcgcatat
      121 cgacgtgacc aaagagaagg ttgcgacgca gacagttaaa gagagcgaac agcgccttct
      181 ggcagtactc aacagcatcg acgcgcttgt ctttgtgacg gatgtcgaca cgttcgagat
      241 tttgatgatc aacgatttcg gcaggaaaat cttcggcgac gtcgtcggca ggagatgttg
      301 ggaggctatt caaaaaggac agacggggcc gtgcgagttc tgcgccgaag ctccgcttct
      361 cggcgacgac ggagaaccgg cgggagtacg gcgttggcag gtattcaacg agcgtacgaa
      421 gcgctggtac gactgccgcg attctgcgat acgctggacc gaccgcaaat acgtacgcct
      481 tgagattgcg atggatatta cggaaacgaa aaagatcgaa gaagagcttc gcgtgcatcg
      541 cgacgaactg gacaggttgg ttgcggtgcg gacggaggaa ttgagcgaga gcaaccgtga
      601 actggaacgg cagacggagt ttgcacagga gatggcgctc aaggcggaag cggcaaacga
      661 ggcgaaaagc gccttccttg caaacgtaag ccacgagatc cgcaccccga tcaacgccgt
      721 gatcgggttt accgaactgc tgctcgactc cacgctttct tccgagcaac gccgccaggc
      781 ggagaccgtc agggcgagcg cggaaacgct gctcttcctt atcaacgaaa ttcttgactt
      841 tgcaaagatt gaagcgggaa agctcgaact ggaagttgtg aatttcgacc tgagaaatac
      901 tgtggagggc tttgcgggaa cgcttgccct tcaggcctac acgaagggga tcgaactcgc
      961 ctgcggcgtc gaaccgtcgc ttccgtcgaa ggtgcgcgga gatcccgtgc gcatccggca
     1021 gattctgatg aatttgggaa gcaacgcggt gaagttcacg caggaaggcg agacggtgat
     1081 ctctgtctcg aaagagtccg aaaccgagga aacgctgtgg atccgttttt cggtgcggga
     1141 tacgggaatc ggtatccccg gagagaaaat ggggcttctt ttcaccaagt tcacccagct
     1201 tgacgcttcg acaactcgcc agtacggagg gacaggcctt ggattggcca tatcgaagca
     1261 tcttgtggag aggatgggcg gttccatcgg tgtggagagt gaacacggaa acggttctac
     1321 gttttggttt gtgcttccct tcgaaaaaaa cggccgcggc gcggaagctc cggaagctcc
     1381 cgaaggcgta gcgggtctcc gtattctcgt cgtcgacggc aacgcggcct cgcaagcctt
     1441 tttcttgcgc cagttcgagt cctggggcat gagagccgtc gtctcctcgc ggacagaggc
     1501 gcttgacgtt ttacggcgtg gtttttcgga cgaagatccc ttccgtgttg tgctcgacgc
     1561 agattcaggc cgttcaggag agcattcgct cggggatgcg atcatgaatg acgagagcct
     1621 ctcggaaact gttctcatca cgcttgtccc tctcggaata cggcacggta tcaatcctcc
     1681 cgacggagag aggcgctatt cattggtgaa gccggtccag agcgccgagc ttgctgcggc
     1741 gctcaccgcg attgccctcg gaggcacggt gttttctgtt gcacacgagg atgggaagga
     1801 atcttcctca tggcttcttt catgcgggga gaacgcgcgt atactgctcg ttgaggacaa
     1861 tgcagtaaac cggcaggtgg cgcttgctct actgaagaag gcgggtgtgc gcgccgatac
     1921 ggccgaaaac ggtctggaag ccttggaagc tctgcgttcg aacgattatg atctggtgct
     1981 tatggacctt cagatgcctg ttatggacgg tctggcggcg acccgggcga tacgggccgg
     2041 gcggggcgat gtgcgcaatc cggacgtgcc ggtggttgcg atgacggctc atgcgctcca
     2101 gagtgacaga gaagcgtgca tgcacgcagg aatgaacggc tacatcacca aacccatcag
     2161 gccggcggag cttctttgcg agcttgagcg caggctctgc gcggaaacgg ccccggcgcg
     2221 agacaatcgg agagaatacg ttacgcggac gggaccgcgc acattcaatt tcgacgatct
     2281 tgtggaacgc cttggcgacg aggagacggc cggcctcatt atggaagcct tcctcaaaga
     2341 gatgccggaa gccatgagcg ccctggagaa gagtctccgg acgggggatt tcctcctctc
     2401 ggggaggaat gcccatacga tcaaaggcgt ggcgggaaac atcggaggag aggcgcttcg
     2461 ggaagcggcc cttcagtttg agcgagcggc caaagacggt gatacgaagc ttcttcatgc
     2521 tcttcgggag cgggtgcatg cggagtattg cgcgctgaag gatgaaatcg aaagaatgct
     2581 gcgaacgctt cgccccccgg agtgagcccg cggggtaccc ttagcaagat catacggaaa
     2641 atggagcgtt catggagttt ctccggagcg ctccattttt atttttaaag ggagagcaag
     2701 aaagacttga caaacgcaca ctcaatactg aaaatatgaa cgagcatttg atggaatagt
     2761 gttttgcttg ttgaaacact ccgtgccgtt tattctctgg ctacgcccgc ctacgggttt
     2821 ccctggcgga ttcaaggcaa caccatcata aaggaggggt ttttgtgaaa cgaggaattc
     2881 tggtagtgtg cgcgctcgcg ttcgtgtttc tgttcgcgct
//
LOCUS       DAOSWW010000262         2916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25353_length_2916_cov_3.927298, whole genome shotgun sequence.
ACCESSION   DAOSWW010000262 DAOSWW010000000
VERSION     DAOSWW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25353_length_2916_cov_3.927298"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PK442_06240"
     CDS             <1..1066
                     /locus_tag="PK442_06240"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOO87284.1"
                     /translation="DLPVLFSSLWVAAVTGNRYALDLLPKQIPLNLAALPAATAEGAP
                     APEKRVDIPEDHLRAVVAAMPGAISSLREKLSAEPESAWFSSIGTAWMHLLGTLASEF
                     GKGYPLYMQSPLFAAKQLETQLGSYTELRHDTILYEKPNYAEMGDGGDEEPPKPLPKG
                     FVEPNLPFWNELLRVVDYIQNGFEKNVLFPMDMEEYGALTQFRQTVQRCGKIAAKQLQ
                     GKALTEEEYEFIRLFSLDYMAAPADGYGAVPPDELFQSGLIVDIQTMNLDPIAIGDPA
                     ILYQATAEPSIMLVLVGNENTPRITIGMAFDHREFTAPHGRRITDSVWKKRVYDKYYD
                     GDPKGLPLPAKNFWYDGLRP"
     gene            1047..1967
                     /gene="amrB"
                     /locus_tag="PK442_06245"
     CDS             1047..1967
                     /gene="amrB"
                     /locus_tag="PK442_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOO87285.1"
                     /translation="MTACVRKLAVLCICLFFAASPLFSEEPPHLQKLRERPFPAAYPG
                     GLFADAIEAAKSTVPASGRVSGLVLPHHLLAADLIAKGFAHVREGGYRRIVILSPDHF
                     SRGKTSFSVPGRDFLTSLGTVPLDKEWTAALLRSELVSESSLFSHEHGVQALLPFIAR
                     HFPGAFVLPVAIGKRSTPEEWRVLAALLAPLVLDDTLIVQSTDFSHYLTREETAKHDA
                     ESLAVLLSADPERVVSLRQPSHIDSRGALFVQMTLQKSLDANPVLLAHASSFEYNPAE
                     AEKTGGTSYIVLAYRHADGEEDDSILAPSP"
     gene            complement(2045..2821)
                     /locus_tag="PK442_06250"
     CDS             complement(2045..2821)
                     /locus_tag="PK442_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87286.1"
                     /translation="MTKRICVLGVCAAAIFGLVLWTAGNASANERNSFMDAVFSAIES
                     AENLNLSDGNMKIASYEGPVSNKDEKFMGLLRAKLRLAHIFRKLEYPEENLSVLKGYE
                     VVRLMIAAASSGSLNTQGAPFDLGDYIVFQLILPLQLEPLIRELPVYYDSMKSKEDWQ
                     EKFDLILETGDEYGPSLNRGKTFHLAGFPKVNTKKEYTMAIDVEFKDESLYYSEADSS
                     IEGWLYSFWLRRYQEGSLETVKVILDWLNTKLDEVEKAVG"
BASE COUNT          563 a          992 c          797 g          564 t
ORIGIN      
        1 ggatctgccg gtgcttttct ccagcctgtg ggtggccgcc gtcaccggga accggtacgc
       61 attggacctc ctcccgaagc agatcccgct gaatctcgcc gcgcttcccg cggccacggc
      121 ggaaggcgcg cccgcccccg aaaaacgggt cgacatcccc gaagaccacc tgcgcgccgt
      181 cgtcgcggcg atgccgggcg cgatctcatc cctgcgcgaa aaactttcgg cagagcctga
      241 gtccgcatgg ttcagctcca taggaacggc ctggatgcac ctgctgggaa cgctcgcctc
      301 cgagttcgga aaggggtacc cgctgtacat gcagagcccg ctcttcgccg ccaaacagct
      361 tgagacgcag ctcggctcgt acaccgagtt gcggcacgac accattctct acgaaaagcc
      421 gaactacgca gaaatgggcg acgggggcga cgaagagcct cccaagccgc ttcccaaggg
      481 cttcgtcgag ccgaatcttc ccttctggaa cgagctgctc cgcgtcgtcg actatatcca
      541 aaacggtttt gagaaaaacg tcctcttccc gatggatatg gaggagtacg gcgcgctcac
      601 ccagttccgg cagacggtgc agcgatgcgg caagatcgca gcgaagcagc ttcagggcaa
      661 ggcgctcacc gaagaagagt atgagttcat ccgtctgttt tcgctcgact acatggccgc
      721 cccggcggac ggctacggcg cggtgcctcc ggacgagctc ttccagtccg gactcatcgt
      781 cgatatccag accatgaacc tcgacccgat cgctatcggc gacccggcca tcctctacca
      841 ggcgaccgcc gagccttcga tcatgctggt gctcgtcggc aacgaaaata cgcctcgaat
      901 cacgatcggc atggcgttcg accaccgcga gttcaccgcc ccgcacggac ggcgcatcac
      961 cgacagcgtc tggaagaagc gcgtctacga caagtactac gacggcgacc ccaaggggct
     1021 gccgctaccg gcgaagaact tctggtatga cggcctgcgt ccgtaaactc gccgtcctct
     1081 gtatctgcct gttttttgcg gcgtctcctc tcttttcgga ggagccgccg caccttcaaa
     1141 aactgcgcga acgcccgttc cccgcggcgt accccggcgg cctcttcgca gacgccatcg
     1201 aggccgcgaa atcaacagtg cccgcttccg gccgcgtctc cggactcgtc ctcccgcacc
     1261 atctcctcgc ggccgacctg atcgcgaagg ggtttgcgca cgtgcgcgag ggagggtacc
     1321 ggcgcatcgt catcctctcc cccgaccact tcagccgggg aaagacgtcc ttctccgtcc
     1381 cgggacgtga ctttctcacg tctttgggaa ccgtcccgct cgataaagag tggacggccg
     1441 cgctgctacg gagcgaactt gtctccgaat ccagcctctt ctcacacgaa cacggcgtcc
     1501 aagcgctcct tccgttcatc gcgcgccact tccccggcgc tttcgtgctg ccggtcgcca
     1561 tcggcaaacg gtcgaccccg gaggagtgga gggtgttggc ggcgctcctc gctccgttgg
     1621 ttctggacga taccctgatc gtccaaagca ccgacttctc acactacctc acccgagagg
     1681 agaccgcgaa acacgacgcg gagagcctcg ccgtcctcct ttcggccgac ccggagcgcg
     1741 tagtctccct gcggcagccc tcgcatatcg actcccgggg agcgctcttc gtgcagatga
     1801 cgctccagaa gtcgctggac gcgaatcccg tactcctcgc gcacgcttcc tccttcgagt
     1861 acaacccagc cgaggccgaa aagacgggcg ggaccagcta catcgtgctc gcctacagac
     1921 acgctgacgg cgaggaagac gacagtattc ttgcgccttc cccctgaacg accgcacccc
     1981 gccttcgacg ggaagacggg gtgcgtgcag tgctgtcgaa gagtttccac agcgggaacc
     2041 gttgttagcc caccgctttt tcaacctcgt cgagcttcgt gttcagccag tccaggatga
     2101 ccttcaccgt ctccagcgag ccctcctggt agcggcgcag ccagaaggag tacagccacc
     2161 cctcgataga ggagtccgcc tccgagtagt agagcgactc gtctttgaac tccacgtcga
     2221 tcgccatcgt gtactccttc ttcgtgttca ccttcgggaa ccccgcgaga tggaaggtct
     2281 tccctcgatt caaagaaggc ccgtactcgt cccccgtctc gaggatcagg tcgaattttt
     2341 cctgccagtc ctccttcgat ttcattgaat cgtagtatac gggcagttcc ctgatgagag
     2401 gctcaagctg cagcggaaga atgagctgga aaacaatata atctcccaga tcgaaaggag
     2461 cgccttgcgt gttcagcgat ccgctggacg cggcggcgat catcaggcgc acgacctcgt
     2521 accccttgag caccgacagg ttttcctccg ggtactccag ctttcgaaag atgtgggcca
     2581 gacgaagctt cgcccgcagc agccccatga acttctcgtc cttgttcgag acgggcccct
     2641 cgtaacttgc tatcttcatg ttgccatcgc tcaagttcag attttccgcg ctctcaatag
     2701 cggagaacac cgcgtccatg aacgagtttc gctcatttgc agatgcgttc ccagccgtcc
     2761 agagcacaag accgaaaatc gccgccgcgc atacaccaag aacacagatt ctctttgtca
     2821 ttgttccacc agcctttcca atgataggag cagtgggccc ccttgctata tcgcgattat
     2881 tctatcatga cgccctcgct cggcggaacg gtgggg
//
LOCUS       DAOSWW010000263         2916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25362_length_2916_cov_2.905278, whole genome shotgun sequence.
ACCESSION   DAOSWW010000263 DAOSWW010000000
VERSION     DAOSWW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25362_length_2916_cov_2.905278"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PK442_06255"
     CDS             <1..671
                     /locus_tag="PK442_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOO87287.1"
                     /translation="AVLATAAYQPFFFTGTAKTGVAVGTLVTLASAPVVSGIVGFFAL
                     GERPQRVWYLSSLLAIGGCSLLTLSGDAESVVHPVGVHFSRGAGFSYAIFVLGSKYLL
                     KEHTPDAVNGVVFSGAGLVLFPVLLMTPPMWLAEPRGLLVALHLGLIATGLAYFLFLK
                     GLSSVPASTSVTLSMAEPLTASLLGVFLLGERMNFLGGIGVFLTFAGVALLALGGSRS
                     AGDS"
     gene            695..2233
                     /locus_tag="PK442_06260"
     CDS             695..2233
                     /locus_tag="PK442_06260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOO87288.1"
                     /translation="MTFDIKTVVSLWLLLSVISALTVVSMYFRYRERFQGIAFWALHM
                     GLYTAGVSLLVLRGGVPDVLSVLLANYLLILSLFFLLNGMERFVGRPGRHFHNYALVA
                     VYALAFWYFFDVRPDMTLRTVLTSLTASAFFGQVTFLLLIRTPSNLRDITRTSAFVMI
                     SYAVLSLARALLLIFSPLETNEFFEAGGLTSVALLCYVGLSVCWLVAIILMIPRRLLE
                     EVSVQEEKFAKIFRSSPGAIMLTRKADGRIFDVNGGFLEMTGYAFEEIVGATVFATKL
                     WGDDKEYADIFSALSRDQGVRGIESVLRKKSGESMSVLFSSDIVVIDGEQCMLSTFSD
                     ITEQSRLRRQLHEMATHDPLTGLPNRRLFDERATDVFARATRQKRKFAIMSFDIDHFK
                     DINDTYGHAVGDEVLRQAAVRLKESLRITDTVARLGGDEFVALIEEVRGRSDASRVAF
                     KLLELFRRPFIVEGHEVLIRVSIGVALYPENGETVEVLLKKSDDALYTAKEMGRDTLK
                     MAED"
     gene            2328..>2916
                     /locus_tag="PK442_06265"
     CDS             2328..>2916
                     /locus_tag="PK442_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOO87289.1"
                     /translation="MDQGFRMEKDSLGEVQVPASKLWGAQTQRSLQNFRIGAEKMPEE
                     IIAAFALLKKAAARTNARLGLLDEVRMRAICGAADEILERTHDDQFPLVVWQTGSGTQ
                     TNMNVNEVIARRATELCGGETVIHPNDHVNRSQSSNDTFPTAMHVSAVLALEDYLLPT
                     LESLRSTLGKKATQFADVVKIGRTHLQDATPLALGQ"
BASE COUNT          581 a          757 c          854 g          724 t
ORIGIN      
        1 tcgccgtgct tgcgacggcg gcataccagc ccttcttctt tacaggaacg gcgaagacag
       61 gggttgctgt tggaacgctg gtcacgctcg ccagcgcgcc ggtcgtctcg ggcatcgtcg
      121 gtttcttcgc tctcggtgag cggccgcaga gagtgtggta cctttcaagt ctccttgcca
      181 tcgggggatg ctcacttctg accttgtccg gagacgccga aagcgttgtg catcctgtcg
      241 gcgtgcattt ctctcgcggg gcggggttct cctatgccat ctttgtcctc ggcagcaagt
      301 acctgttgaa ggaacacacc cccgacgccg tcaacggggt ggtcttttct ggagcggggc
      361 ttgtgctgtt tcctgtgctt ctcatgaccc ccccgatgtg gcttgccgaa ccgcggggat
      421 tgcttgtcgc gcttcacctg ggactcattg ccaccgggct cgcgtatttt ctctttctta
      481 aaggactctc ctccgttccc gcctcgacca gcgtcacgct ctccatggcg gagccgctga
      541 ccgcttctct gctcggggtc tttctgcttg gcgagcgaat gaacttcctc ggtggaatcg
      601 gcgtcttcct cacgttcgcc ggggtggcgc tgcttgcgct cggaggaagc cgcagtgcag
      661 gagattcctg actcgggacg aagcggaggt tcccatgacg tttgacatca agaccgtagt
      721 gtcgttatgg ctcctcttga gcgtaattag cgctctcacg gttgtttcca tgtacttccg
      781 gtatcgcgaa cgttttcaag gaattgcttt ttgggctctc catatggggt tgtacacagc
      841 cggtgtgtcg ctgctggttc ttcgcggcgg cgttccggat gttctttcag tacttcttgc
      901 caattatctt cttatcctct ccctgttttt tcttctgaat ggaatggaac gatttgtcgg
      961 aagaccggga cgtcatttcc ataactacgc tctggtagcg gtgtatgctc tggctttctg
     1021 gtactttttc gacgtccggc cggatatgac gctccgtact gtgctgacgt cgctcacagc
     1081 ctctgcgttt tttggccagg ttactttcct tctgctgata cgaacgcctt cgaatctgcg
     1141 cgatattacc aggacgagcg cgttcgtcat gatttcttat gcagtgttga gccttgcccg
     1201 ggcgcttctc ctcatttttt ctcccctgga aacgaacgaa ttcttcgaag ctggagggct
     1261 cacttcagtt gcgctgctct gttatgtcgg tctgagcgtc tgttggctcg tcgcgattat
     1321 cctgatgatt ccccgccgcc tgctcgaaga ggtcagtgtc caagaggaaa agttcgcgaa
     1381 gatattccgt tcatcccctg gcgcgatcat gcttacgaga aaagcggacg ggagaatttt
     1441 cgacgtgaac ggcggttttc ttgagatgac aggatatgcg tttgaagaga ttgtaggcgc
     1501 cactgtcttt gcaaccaaac tgtggggaga tgacaaggaa tatgccgata ttttctctgc
     1561 cttgtcaaga gaccagggcg tacgggggat cgagagtgtc ctccggaaaa aatcgggaga
     1621 gagcatgagc gttcttttct cttcggatat tgttgtgatc gacggcgagc agtgtatgct
     1681 gtcgactttc tcggatataa cggagcagag ccggctgcgg cggcagctgc acgaaatggc
     1741 cacgcatgat cccctgacag gactgcccaa tcgcaggctt ttcgatgaac gggcaaccga
     1801 cgtcttcgca cgtgcgacga ggcaaaaacg caagtttgcc ataatgtcct tcgacatcga
     1861 ccacttcaag gacattaacg atacctacgg acacgcagtc ggggatgaag tgttgcggca
     1921 ggcagccgtt cgtctgaaag aatctctgcg tataaccgat accgttgccc gccttggcgg
     1981 cgacgagttc gtcgctttga tagaggaggt tcgtggacga agcgacgcgt caagggtggc
     2041 cttcaaactc ctggaacttt tccggaggcc gttcattgtg gaggggcatg aggtgctcat
     2101 cagggtgagt ataggcgttg cgctttatcc ggaaaatggc gaaactgttg aagtcctttt
     2161 aaagaagtcc gacgatgcgc tctatactgc aaaggagatg gggcgggaca ctttgaaaat
     2221 ggcggaagac tgagaaaaca aggatgcttt tgggttcttt tggggtgaac ggtaccaatg
     2281 ggcgcgaaac gaaaaagagc gtgaagccgc gcaaggaggg tatgcttatg gatcaggggt
     2341 ttcgaatgga gaaggactct ctgggagaag tgcaggtacc tgcttcaaaa ctgtggggcg
     2401 ctcagaccca gagaagtttg cagaatttta gaataggcgc ggaaaaaatg cctgaggaga
     2461 tcatcgccgc atttgctctg ctgaaaaaag ccgccgcgag aacgaacgcc cgtctcggtc
     2521 tgcttgacga agtacgtatg cgggctattt gcggagcggc cgacgaaatc ctggagagga
     2581 cgcatgacga tcagttcccc ctcgtcgtct ggcagaccgg cagcggaacc cagacgaata
     2641 tgaacgtcaa cgaagtgatc gcgcggcgcg ccacggaact ttgcggcggg gagacggtca
     2701 ttcacccaaa cgatcatgta aatcggtcgc aaagctccaa tgacaccttc ccgacggcca
     2761 tgcacgtttc ggcggtattg gcattggagg attatcttct tccgactctt gaaagtcttc
     2821 ggtcgacgct cggcaaaaag gcgactcagt tcgccgacgt cgtgaagata ggaagaacgc
     2881 acctgcagga cgctactccc cttgcgctcg ggcagg
//
LOCUS       DAOSWW010000264         2912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25410_length_2912_cov_3.871894, whole genome shotgun sequence.
ACCESSION   DAOSWW010000264 DAOSWW010000000
VERSION     DAOSWW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25410_length_2912_cov_3.871894"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="PK442_06270"
     CDS             complement(<1..1313)
                     /locus_tag="PK442_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOO87290.1"
                     /translation="MPLFLGVDLGTSSLKAAVLDEKGNIVARAKVPSVMNSPEKDFYE
                     VDAEATWWDGFLSLCSEAGKIVSLREIAAVCVSSVCASFVPVDEGGVPMHDAILYGID
                     KRASRQIERLNRRYGASLADRLGGMFTTHSILPKAIWLKEERPDAFRRTKYFMEPNNF
                     VTFRLTGERAWDYPSAAAAGLVDREKLTWAEDIFNEQGVSARSFAELRWPLSVLGSIS
                     EDASARTGLPAGIPVITGACDINAEAAAAGALSVGECVVVFGSTVSILLNTERPVRLP
                     GFVSGISVVEGMYRLGAATSSGSRFLQWVHSVIRGGDGKSVSSPTGILMVPWLDGART
                     PFHDPMAKMLFQGMDSGTSGGALLRAGKEALGYELDSLFAMLEQAAPVPETISVSGGL
                     SNDVELMEIVSAITGKKLRLHPSVDAACGDALLAAAGSGYVSLAD"
     gene            complement(1395..2324)
                     /locus_tag="PK442_06275"
     CDS             complement(1395..2324)
                     /locus_tag="PK442_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOO87291.1"
                     /translation="MKKYGVVVAVACLAALLVTGVAAAKQITVGFIATNFSAEAQARV
                     ANAFEKMAKEKNWDVKMLNSTGSIETQSNQLENLYQMKVDAVVMAMAHPLEIRPALDK
                     VLSANIPVITIDSGYVEGVVADITADNFAMGAKASTFLMDSIGGQGNIVVIKFEKHYG
                     TRRRGKVLDVVLSEYPGVKVLAEYSVVATKRFMDDTRAAMETYVTRFGDQIDGVWCAF
                     DQLAYVAGDVLQERGIKAVVVGTDGNQETMRRIGAGQMSATVAQPFEAMAEKAVELVQ
                     AIAAEGKSIEEATGGRKIFYMDAPLVDKTSLPE"
     gene            complement(2375..>2912)
                     /locus_tag="PK442_06280"
     CDS             complement(2375..>2912)
                     /locus_tag="PK442_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87292.1"
                     /translation="LPIRVFEMPPSFLALGRGMVGPVPSLIFIMFVVVAVAQVFISKT
                     RIGKYMQATGANQSASRLAGINTDRCKILALSLSGLAAAFTGILLTSRLGSANPEGAS
                     GFMMDAFASALLGMTVLSVGRANPFGTFIGALMIGVINNGMTLAGAPYYTQDIVKGLI
                     ILLSVTMTSLQAKRMAGN"
BASE COUNT          597 a          953 c          809 g          553 t
ORIGIN      
        1 caatccgcaa gcgaaacata ccccgagccc gccgccgcaa gcagcgcgtc gccgcaggcc
       61 gcgtcgaccg aagggtgcag gcgcagtttt tttcctgtga tcgcggatac aatctccatc
      121 aactcaacgt cgttgctcag accgcccgag acgctgatcg tttccggcac gggagccgcc
      181 tgttccagca tggcgaagag cgagtcgagt tcgtacccca acgcctcctt gccggcgcgc
      241 agaagcgcac ctcccgaagt cccgctgtcc attccctgaa acagcatctt cgccatcgga
      301 tcgtgaaacg gcgtccgcgc tccgtccagc caggggacca tcagaatgcc ggtgggagaa
      361 gatacagatt ttccatcccc gccccgaata acggagtgca cccattgcaa aaagcgcgac
      421 cccgaagagg tggccgcgcc gagccggtac atcccttcca caacggatat tcccgaaacg
      481 aaccccggca accggacagg gcgctccgtg ttcagaagaa tgctgaccgt cgagccgaag
      541 acgacgacgc actctccgac gctcaaggcg cccgccgcgg cggcctccgc gttgatgtcg
      601 cacgcgcctg tgattaccgg tatccctgcg gggagaccgg ttcgcgccga cgcatcttcc
      661 gaaatgcttc cgaggacgga gagcggccac cggagctccg cgaaggaacg tgccgaaacg
      721 ccctgttcgt tgaaaatatc ctccgcccac gtcagtttct ctctgtccac aagtccggca
      781 gcggcggcgg aagggtaatc ccacgcgcgt tcgccggtca gccggaaggt gacaaagttg
      841 ttcggttcca tgaagtactt cgtccgcctg aacgcgtcgg ggcgctcctc cttcagccaa
      901 atcgccttgg gaaggatgga gtgcgtggtg aacatgcctc cgagacgatc ggccagcgag
      961 gcgccgtagc gccggttgag ccgttctatc tggcgcgagg cccgtttgtc gataccgtag
     1021 agaatggcgt cgtgcatcgg aacgccccct tcgtcgacgg gaacgaacga agcgcagacg
     1081 gagctgacgc acaccgcggc gatctcgcgc aaagaaacga tttttcccgc ctccgaacaa
     1141 agcgaaagaa aaccgtccca ccaggtcgcc tcggcgtcca cctcgtaaaa atctttttcg
     1201 ggggagttca ttacgctcgg gacctttgcc cgcgcaacga tattcccctt ttcgtccaga
     1261 acggccgcct tcagcgacga cgttccgagg tccactccaa gaaaaagagg cactgcatac
     1321 acctcctgaa aaacggcgcg gagagcatgt gatcgccatc cgcgccgaaa tgctgcgtac
     1381 gtggaagaaa ggaactattc cggaagcgac gtcttgtcga cgagcggcgc atccatgtag
     1441 aatattttcc gcccgccggt agcttcttca atcgattttc cttcggccgc tatggcctgc
     1501 acaagctcaa cggctttctc cgccatcgcc tcgaaaggct gcgccacggt tgcggacatc
     1561 tggccggccc ctatcctccg catggtctcc tggtttccgt ccgttccgac caccacggcc
     1621 ttgattcccc gctcctgcag cacgtccccc gcgacgtacg caagctggtc gaaggcgcac
     1681 catacgccgt cgatctgatc gccgaagcgg gtaacgtacg tctccatcgc cgctctggta
     1741 tcgtccatga agcgtttcgt cgcgacgacg ctgtactcgg cgagcacctt cacgcccgga
     1801 tactccgaga gaacgacgtc gagcaccttc cccctgcgcc tggttccgta gtgtttctcg
     1861 aacttgatga cgacgatatt cccctggccg ccgatgctgt ccataaggaa cgtggaggct
     1921 tttgccccca tagcgaagtt atcggcggta atatcggcga cgacgccctc cacgtacccg
     1981 gagtcgatcg tgatcaccgg aatgttcgcg gagagcacct tgtccagcgc gggacggatc
     2041 tccagcggat gcgccattgc catcaccacg gcgtccacct tcatctggta gaggttctcc
     2101 agctgattcg actgcgtttc gatggacccc gtcgagttga gcatcttgac gtcccagttc
     2161 ttctccttgg ccatcttttc gaacgcgttg gccacccgcg cctgcgcttc ggcggagaaa
     2221 ttcgtggcga tgaaccccac ggttatctgc ttcgccgccg cgactcccgt gacgagcaac
     2281 gctgccagac acgctacagc aacaacaacc ccgtactttt tcattccctt tccctccttg
     2341 aattgtggtt tcctgtctat aaaagaaggc gaggtcagtt ccccgccatt ctttttgcct
     2401 gaagggaggt catcgtcacc gaaagcaata tgatcagtcc cttcacgata tcctgagtgt
     2461 agtacggcgc gcccgcgagc gtcatgccgt tattgatcac gccgatcatc agcgcgccga
     2521 tgaacgtccc gaagggattc gcccgtccca cgctgagcac cgtcatccca agcagcgctg
     2581 aggcaaacgc gtccatcatg aacccggagg ctccttcagg gttggccgac ccgagacggg
     2641 aggtgagcaa gatgccggtg aaggccgcgg caagaccgga aagtgagagc gccagaatct
     2701 tgcatcggtc cgtgttgatg cccgcaagtc gggaagcgga ctgattcgcc ccggtagcct
     2761 gcatgtactt gcctatacgg gtcttcgaga tgaacacttg cgcgaccgcc actacgacaa
     2821 acatgatgaa aatgagagag ggtaccggcc cgaccatccc ccgcccgagc gcgaggaacg
     2881 aagggggcat ctcgaagacc cgtataggca ag
//
LOCUS       DAOSWW010000265         2911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25417_length_2911_cov_5.111695, whole genome shotgun sequence.
ACCESSION   DAOSWW010000265 DAOSWW010000000
VERSION     DAOSWW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25417_length_2911_cov_5.111695"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(412..1149)
                     /locus_tag="PK442_06285"
     CDS             complement(412..1149)
                     /locus_tag="PK442_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87293.1"
                     /translation="MNQWIAFWNILLKDMRTYYMKPPNISWGLLFPLAWTGMFFIRSG
                     SGLESIPALLPGVTALSILFGSTSVLAVTVTFEKKNRSFERLLLAPISFELLMLAKTS
                     GAILFGVLNAFVPVLMASFIADLSPIDWRAFVPAVVLLSTASTFLGLFIAVAVSEVFE
                     AQTFSNFFRFPMIFLCGLFFPIERLPVFLRPLSYALPPTYGVDMLRGAVGGVHTMPYL
                     LNLAALCVFCAGLFALSLRTIRRSWIA"
     gene            complement(1163..1570)
                     /locus_tag="PK442_06290"
     CDS             complement(1163..1570)
                     /locus_tag="PK442_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOO87294.1"
                     /translation="MQKNTLLASDALIYKDLVESSPDLICRFLPDTTVTFANEALCRA
                     LEIDRSELLGRHFSELIPPEEVQPAREQVASLSADGHADILQRRHERLDGSGYPQGLK
                     SAEIPLEARSVAVADTVEATAPHPRSCTNERPA"
     gene            complement(1790..>2911)
                     /locus_tag="PK442_06295"
     CDS             complement(1790..>2911)
                     /locus_tag="PK442_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO87295.1"
                     /translation="GAEEGDPFHVAVIDMHMPLMDGETLGRTIKADPQLAKTCMIMLT
                     SLGMRGDARRFADAGYSAYLTKPVRCRELMGVVVPALRERYWSETESAPLLTRHTVRK
                     QLEPCDTKVRVLLAEDNVTNQQVAVGILEKFGIEVEIARNGEEAVEAVRRRSFDLIFM
                     DIQMPLMDGLEATKRIRGMHAASGEPAVPIVAMTAHAMEGDRERCIEAGMNDYLSKPV
                     KPAAMFAMLRKQLPAKDLLPCTYTASSAVDDKKVDADVSVWNRTPFYDRLLEDRELAH
                     RILEDFLIDIPSQIRQLRATLKKGDSEGARRRAHAIKGAAASVNGEFLRLAALELERR
                     ALAGEQQELAFLADRLEASFEDLRAAMLKELRNRTKSHP"
BASE COUNT          598 a          851 c          857 g          605 t
ORIGIN      
        1 aacggcgtcc cgtccggccg cgtgacgacc gaggcgacgc ccgtcgcgtc cgagaactcg
       61 tcctgcagac gctggatgtc ggcgaggtcg aacatgtcct cgaagcggat ttcgtaagag
      121 ctgtccgtta caaaatcgat gtccatggaa aagacctccg gagatagtcg tttattcacg
      181 ctacttcctt ccgggagaga tgcggcaatt ccccaaagag cgcgcagaga gagttttctg
      241 cggcgccccg tatgcgcata cacaatcttc cgatggtttg tattatagac tgtcaagggg
      301 tatttcgcac cttttgcctt ccaaggcgag cgcaagaaag agtgtgcgct tccaaaaaag
      361 aggaggtgca agagaatcac gcagttattc gagagaaaag gaggcggcgc gctacgctat
      421 ccagctcctc cggatggtcc gcaggctgag ggcgaagagc cccgcgcaga agacgcagag
      481 cgcggcgagg ttcaggaggt acggcatggt atggacgccg ccgacggccc ctctgagcat
      541 gtccactccg tacgtcggag ggagcgcgta ggagagcggg cgcaagaaga cgggcagcct
      601 ctctatgggg aagaagagtc cgcagaggaa gatcatcggg aagcggaaga agttggaaaa
      661 cgtctgcgcc tcgaagacct cgctgaccgc caccgcgatg aagagcccga gaaacgtgga
      721 cgcggtcgac agcagcacga ccgccggcac gaacgctctc cagtcgatcg gcgataaatc
      781 cgcgatgaag ctcgccatga gcaccgggac gaaggcgttc agcacgccga agagaatggc
      841 cccggacgtc ttcgccagca tcagcagttc gaaggatatg ggcgcaagca gcagccgttc
      901 gaacgagcgg ttcttcttct cgaacgtcac cgtcaccgcg agcaccgagg tggagccgaa
      961 gaggatggag agtgccgtca cccccggaag cagcgcggga atactctcca ggccgctccc
     1021 cgaccggatg aagaacatgc cggtccaggc gagtggaaag agcagccccc agctgatgtt
     1081 gggcggcttc atgtagtacg tgcgcatatc cttgagcagg atgttccaga acgcgatcca
     1141 ctggttcacg gctattcctc ccttatgcgg gtctctcgtt agtgcaggag cgcgggtgcg
     1201 gcgccgtcgc ttccaccgtg tcggccacgg cgacgctcct cgcctcaagg ggaatttccg
     1261 cgctcttcag tccttgcgga tacccggagc cgtccagccg ttcatggcgc cgctgcaaga
     1321 tatcggcgtg tccgtcggcg gagagggagg ccacttgttc acgggcgggc tgcacttcct
     1381 ccggagggat caactccgaa aagtgacggc caagcagttc gctccggtcg atttcaagcg
     1441 cccggcagag cgcctcgttg gcgaaggtga cggttgtatc gggcagaaaa cggcagataa
     1501 ggtccggact cgactcgacg agatctttat agatgagagc atcggaagca agaagagtgt
     1561 ttttttgcat agtgaaacct ccgcgtcgag ttcggtttga gctccggata ctgttctccc
     1621 tttttcaaaa acaaaaacga ggcgttccag aacgcctccg gtcgtcggct cataccttgt
     1681 atgacatttc tatcacattt aatgtatttt cacaatatgt ctttatgttc tttcgttcaa
     1741 cttccctgaa cctagaagta aaaagagtga gtccttcatt tggatagtgc tatggatgcg
     1801 attttgttct gttccgcaac tccttcagca tcgctgcccg caaatcttca aaactggctt
     1861 cgagacggtc cgccagaaat gcgagttcct gctgttctcc ggcgagcgcg cggcgttcca
     1921 gttccagagc ggccagccgt aggaactctc cgttgacgct tgcggcggcg cccttgattg
     1981 cgtgcgctct tctgcgcgcc ccttcggaat cgcctttttt cagcgttgcg cgcagctggc
     2041 ggatctggct cggaatatcg attaaaaaat cctccagaat tctgtgcgcg agctctctgt
     2101 cctccagcag acggtcgtaa aacggcgtcc ggttccagac ggatacatcg gcgtcgactt
     2161 tcttatcgtc aacagcggaa gaggcggtgt acgtgcaggg gaggagatcc ttcgcaggga
     2221 gctgttttcg cagcatagcg aacatcgccg ccggttttac aggtttggaa agatagtcgt
     2281 tcatccccgc ttcgatgcag cgctcccggt ctccttccat cgcgtgcgct gtcatcgcga
     2341 cgatcgggac ggcgggctct cccgaggcgg catgcatacc gcggattctt tttgtggctt
     2401 cgagcccgtc catgagcggc atctgaatgt ccatgaagat caggtcgaag ctgcggcgcc
     2461 ggacagcctc gacagcctcc tcgccgttcc gggctatctc gacctctatg ccgaacttct
     2521 ccagaatacc caccgccacc tgctggtttg tcacattatc ttcggcgagc aaaacacgta
     2581 ctttcgtatc gcagggctcc aactgctttc ggacggtatg gcgcgtcaga agaggcgcgc
     2641 tctccgtctc gctccagtat ctttcccgga gcgccggaac aacgaccccc ataagctctc
     2701 tgcaacgcac cggcttcgtc aggtacgcgg agtaccctgc gtccgcgaag cggcgtgcgt
     2761 cgcctctcat tcccagcgag gtgagcatga tcatgcacgt tttggcaagc tgcgggtccg
     2821 ctttgatcgt ccggccgagc gtctctccgt ccatcagggg catgtgcata tcgatgacgg
     2881 cgacgtggaa cggatcccct tcctccgctc c
//
LOCUS       DAOSWW010000266         2909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25447_length_2909_cov_4.736861, whole genome shotgun sequence.
ACCESSION   DAOSWW010000266 DAOSWW010000000
VERSION     DAOSWW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25447_length_2909_cov_4.736861"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PK442_06300"
     CDS             <1..554
                     /locus_tag="PK442_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO87296.1"
                     /translation="LHTIRRNPGITNLVFNNMVYGLTKGQASPTSPRGFRTSVQVDGV
                     HAQPFNPLATALVQGAGFVARAYAGDVEKTKDVIKEAVQYKGYALVDIFQPCVSFNSV
                     NTYAWFKENTYWLDGHDPSDFSKALSLALSEEPFPLGVLFRGEERTTFEGSLAAYRND
                     KNPLFRRAAPREVVKKLLDGMCR"
     gene            568..1029
                     /locus_tag="PK442_06305"
     CDS             568..1029
                     /locus_tag="PK442_06305"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:691..693,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOO87297.1"
                     /translation="MEGKPVPGFLLQDGSGKEFSHKDFSGSWLALFFYSKDNTSGUTN
                     EVKDFADRMSAFEELNCRIVGVSPDSVESHRKFAAKLDISFPLLSDPEHEFIEACGFW
                     KQKKMYGKEYMGVERSTLLVTPDGIVARLFSKVKVAGHAEDVLHALKERAG"
     gene            complement(1073..1810)
                     /locus_tag="PK442_06310"
     CDS             complement(1073..1810)
                     /locus_tag="PK442_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87298.1"
                     /translation="MRDGKLLLRITLVLVLCLLARQSSAYVKILTAPGHPVSLIVEHD
                     EGRIRKAFFRSPEGVRPLEHIEQHLLLSDSLTFLCADDDILDDLVWKIDLLQPKTEKM
                     LSLWITSVTEASTAWLALAPAGAGFWESLPRAPLPREDVFLYIAPHLPAYQELGQRSG
                     PEILTFIYTMLLTKNGPKLAAVPEIYRQFLPLTALVCRAQENKDLKAAYTALHQDFER
                     MGQGGMPSREAIDNFLWKRILTVRWKR"
     gene            complement(1970..2617)
                     /locus_tag="PK442_06315"
     CDS             complement(1970..2617)
                     /locus_tag="PK442_06315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO87299.1"
                     /translation="MNEHTVRTTILLVRHGECRGNVDGLFRGRVDFPLNETGLLQAEE
                     VAGALASYRPEAVFSSPLLRALQTAESITAASSSHLFIEDGFNNICLGTWEGRPKKEI
                     AALFPEMWRTWLENPEALSVEGAESLNDVLVRSKKSLDRIVAEYQGKTVVAVTHRTVV
                     KPLLAGCLGIPSPYFWKTHMDTAAYSVLHFDDLHGYSLFCLNQTNHLSRFTTEWI"
BASE COUNT          685 a          719 c          823 g          682 t
ORIGIN      
        1 ttttacatac gatacgaaga aatccgggga tcacgaacct ggtattcaac aacatggtgt
       61 acggtctgac caaggggcag gcttccccga ccagtccgag gggttttcgt acgtccgtgc
      121 aggtggacgg cgtccatgcg cagcctttca accccttggc cacggcgctt gttcagggag
      181 ccggtttcgt cgcaagggcc tatgcgggcg acgtcgaaaa gacgaaggac gtcatcaagg
      241 aagccgttca gtacaagggg tatgcccttg tcgatatctt ccagccgtgc gtctccttca
      301 attcagtgaa cacctacgcg tggttcaagg aaaacacata ctggctggac gggcacgatc
      361 cttcggattt ctcgaaggct ctctcgcttg ccctgtccga ggagcctttt ccgctgggag
      421 ttctttttcg cggagaagaa cggacgacct tcgaggggtc gctggccgca tacaggaacg
      481 acaaaaatcc gctcttccgc cgcgctgcgc cgcgagaggt cgtgaagaag ttactcgacg
      541 gaatgtgccg ttaaggaggg aaaaagaatg gaagggaagc ccgtgccagg ttttctcctt
      601 caggatggat cagggaagga gttttcgcat aaagattttt cagggagctg gctggcgctc
      661 tttttttaca gcaaggacaa tacgtcgggg tgaacaaacg aagtcaagga ctttgcggac
      721 aggatgtccg cttttgaaga gctgaactgc cgaattgttg gtgtgagccc tgattcggtc
      781 gaaagtcacc gcaagtttgc tgcaaaactt gacatatcct ttcccctttt gagcgatcct
      841 gagcatgagt tcattgaagc atgcggtttc tggaagcaga agaagatgta cgggaaggag
      901 tatatgggag tcgaacgttc tacgttgctt gtcacccctg acgggatcgt tgcccgtctc
      961 ttctcaaagg tgaaagtagc cggtcacgca gaagacgttc ttcatgccct gaaagaacgt
     1021 gcagggtaaa aaacggggcg ccgccgaaac ggggcgcccc gcacttcttc cgtcatcgtt
     1081 tccatcgaac agtaagaatg cgtttccaca gaaagttatc tatggcctcc cgtgaaggca
     1141 ttcctccttg ccccatccgc tcaaagtcct gatgcaaggc cgtgtaggcc gctttgaggt
     1201 ccttgttttc ctgtgcccgg cagacaagag cggtcagggg aagaaactgt ctgtatattt
     1261 ccgggacggc cgccagtttc gggccgtttt tcgtgaggag catcgtataa atgaacgtca
     1321 ggatttccgg tccgcttcgc tggccgagct cttggtacgc agggagatgc ggcgcgatgt
     1381 agagaaaaac gtcttccctg gggagcggcg cccgaggaag cgattcccaa aagccggcgc
     1441 ctgccggcgc cagtgcaagc caggcggtag atgcttcggt gacggatgtt atccagagag
     1501 agagcatctt ttcggtcttc ggttgcagga ggtcgatttt ccacaccaag tcgtcaagga
     1561 tgtcgtcgtc tgcgcacaga aaagtaagcg aatccgaaag aagaagatgc tgttcgatgt
     1621 gttccaaggg acgtactccc tccggcgaac ggaagaacgc tttccggata cgtccctcgt
     1681 catgttcgac aatcaaagat acggggtgtc cgggggccgt cagtatcttt acgtaagcgg
     1741 aactctgccg tgcaaggagg cagaggacga gtacaagtgt gatgcgaagc aaaagttttc
     1801 cgtctctcaa gaaggcagcc tcccggatga aaacatgagt ccgaatgaag cgccggagca
     1861 cttgcagtcg ggttttgcgg ttcttcttct ccgtatcgta taatactata tttttcgaaa
     1921 aaggtggagt atagctggtc ttttcttttc aatgccgccg tttcccgcct taaatccact
     1981 cggtagtaaa acgtgaaaga tgattcgttt ggtttaaaca aaagaggcta tacccgtgaa
     2041 gatcgtcgaa atggagcact gagtaggcag cggtatccat atgggttttc cagaagtagg
     2101 gagaaggaat accgaggcat cctgcgagaa gcggtttgac aacagttctg tgtgtgactg
     2161 cgacgacggt tttcccttgg tattcggcaa ctattcggtc aagacttttt tttgagcgca
     2221 caaggacatc gttcaaagat tccgccccct cgactgaaag cgcttccggg ttttcaagcc
     2281 aggtcctcca catctcaggg aatagtgcgg ctatctcttt cttgggacgc ccttcccaag
     2341 tcccgagaca gatgttgttg aacccatcct ctataaacaa gtgagaagat gacgccgcgg
     2401 ttattgattc ggcggtctgc aaggctctga gaagaggact cgaaaagact gcttccggcc
     2461 gataagaagc gagggcgccg gcaacttctt cggcttgcag gagtccggtt tcgttcaaag
     2521 gaaaatcaac ccttccccga aaaagcccgt caacatttcc tcggcactcc ccgtgacgca
     2581 cgagtaagat ggtggtgcga acggtgtgtt cgttcatttt tccctccgtc tccgcagtct
     2641 cgtggagtcg acgacggaag agagcttcat cgtggatctg ccgcgatagc gggaaaaaaa
     2701 ataaaagcct ttgaccagtc ttgacaatca tatcatggat tgttactcta ccttccggga
     2761 gcgatgcatt ggcactcaca ttgaaagagt gctaacgcaa cgaggaggtg tcgaaatgct
     2821 tacggagagg cagctcgaag ttgttttttc cgttgtctac gagtatatcc agaccggaga
     2881 gcccgcgggc tcccggacga tttcgaaga
//
LOCUS       DAOSWW010000267         2907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25481_length_2907_cov_3.941445, whole genome shotgun sequence.
ACCESSION   DAOSWW010000267 DAOSWW010000000
VERSION     DAOSWW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25481_length_2907_cov_3.941445"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PK442_06320"
     CDS             <1..851
                     /locus_tag="PK442_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOO87300.1"
                     /translation="LLPPKEKVFSLSDLDTYVSCPFRYYCDRVASLDTPPEELHRFDL
                     AGSALHLLWQKVWERRLSTGEQLDFLVNGLFDEAMDEKYARLLASENLKRDRKELLRR
                     AVRLARVQQTMEDGGLASGRLEQKREYTLPEFERGGVRFKGRCDRVDFLKGGTAAIFD
                     YKSGPSSNYKESLQLAAYGIALAASGIRTVASAFLCLADGKEKGASVEGAPSVFASFT
                     TPLAPLESDAEEALERVAESFATGLFPPLYESKSCRFCAYSSLCRRRDFHSAESEEEG
                     EENGGR"
     gene            838..>2907
                     /locus_tag="PK442_06325"
     CDS             838..>2907
                     /locus_tag="PK442_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO87301.1"
                     /translation="MAGGDLLARLKAHIASEGALLPAQEEAVFSDAALTLVGAGAGTG
                     KTHTLSWRFIRALLREDVRPRDILTLTFTDKAANEMRERIGELFTKLRTVLDPGGNEL
                     ASVAAELQEAQISTIHAFALNIIREEALFLPSGLGARPVSPPEAELFTARAEKALDTL
                     DFGWFTRNLPQGTSSGDFLDTAAGEYLADAVTEYSPEAAVSFALSLADLLESRGESPR
                     ELLQRADDPHYFDAVKNRIRDICLPLCGEVAGEWERVFAQLPSALPGKGAFNERLDAL
                     RKEWPEDALPPEEPDGLLAFATRLFDGILGNLRGATTSQSGKLAQEALGCSFAEHRER
                     FGTLWTGLRFLSQEPPEFDVRLRSVLLRTSAMVWETYREFRRRRGLLSYDDMIRLAAD
                     IAASENSARSVRTFREILVDEYQDTNPLQDRLIDSVAAEGCRRFLVGDPKQSIYRFRH
                     ADPTLFGERIASPSEETRYIPLQTSFRTRPALLKEINGLFGGIWRNGIASTLPTPYEE
                     LLFPENPEMRELREETSLPPLLPVFRRQRENEPVAETRKRVALALGEKLLELHGQPVW
                     NKKNKQMLPAEWRDMTILVPSRSSFAALEETLHPLFGIPVAFEKGKRYFDRGEIGDLT
                     TAIRMLAFPDDRAAALGFLASPFSGLNMEQFARFLGPEAPSLAEVFPETAARIEELRS
                     VARYSGLF"
BASE COUNT          475 a          947 c          985 g          500 t
ORIGIN      
        1 ccctcctccc tccgaaggag aaggtgtttt cactgagcga cctggacacg tacgtctcgt
       61 gcccgttccg ttactactgc gaccgcgtgg cgtcgctgga tacacccccc gaggaactgc
      121 acaggttcga cctcgccgga agcgcgctcc atctgctgtg gcagaaggtg tgggagcggc
      181 gcctttcgac cggggagcag ctggacttcc tggtcaatgg acttttcgac gaggcgatgg
      241 acgagaagta cgcccgcctc ctggcgagcg agaacctcaa gcgcgaccgg aaggagctgc
      301 tgcggcgcgc agttcgcctg gcgcgcgtgc agcagacgat ggaggacggc gggctggcct
      361 ccgggaggct ggagcagaag cgggagtaca cgcttccgga gttcgagagg gggggcgtcc
      421 gcttcaaggg gcgctgcgac cgggtcgact tcctgaaggg cggcacggcg gccatcttcg
      481 actacaagag cggaccgtcg tcgaactata aagaaagcct ccagctcgca gcctacggga
      541 tcgccctcgc cgcgagcggc atccggactg tcgcgtcggc gttcctctgc cttgcggacg
      601 gaaaggagaa gggggcctcc gtggagggcg cgccgtccgt cttcgcgtcg ttcaccacgc
      661 cgctcgcgcc gctcgagtcg gacgcggagg aggcgctcga acgggtggcg gagtcgtttg
      721 ccacagggct gttccccccg ctgtacgagt cgaaaagctg ccgtttctgc gcctattcgt
      781 cgctctgccg gaggcgcgac ttccatagcg cggaatccga ggaggagggt gaagagaatg
      841 gcgggcggtg atctgctggc gcgtctgaag gcgcatatcg cgtcggaagg ggctctcctt
      901 ccggcccagg aggaggccgt cttctcggac gcggcgctga ccctcgtcgg cgcgggcgcg
      961 gggacgggaa agacgcatac gctttcgtgg cgctttatca gggcgctgct ccgcgaggat
     1021 gtgcgccccc gggacatcct gacgctcacc tttaccgaca aggcggcgaa cgagatgcgg
     1081 gagcgcatcg gcgagctctt tacgaaactg cgcaccgtgc tggaccccgg cggaaacgag
     1141 ctggcctcgg tcgccgccga gctgcaggag gcgcagatct ccaccatcca cgccttcgcg
     1201 ctgaacatca tccgcgagga ggcgctcttc ctcccctcgg ggctcggcgc tcgcccggta
     1261 tcgcccccgg aggcggagct cttcacggcg cgggcggaaa aggcgctgga cacgctggat
     1321 ttcggctggt tcacgaggaa tcttccgcaa ggaacgagct cgggcgattt tctggataca
     1381 gctgccgggg agtacctcgc cgacgcggtg accgagtatt cgcccgaggc ggcggtatcg
     1441 ttcgcgctct cgctggccga cctgctggag agccggggag agtctccccg agagctgttg
     1501 caacgcgcgg acgacccgca ctacttcgac gcggtgaaga atcgcattcg cgatatctgc
     1561 cttcccctct gcggcgaggt cgccggggag tgggagaggg tctttgcaca gcttccgtcc
     1621 gctctgccgg ggaaaggcgc gttcaacgag cggctcgacg ccttgcggaa ggagtggccc
     1681 gaggacgctc tgccgccgga agagccggat ggactgctcg ccttcgcgac tcggctcttc
     1741 gacggcatcc tcggcaatct caggggtgcg acgacgagcc agagcgggaa gttggcgcag
     1801 gaggcgctcg gctgctcctt tgcggagcac cgggagcgct tcggcacgct ctggacggga
     1861 ctgcgcttcc tgtcgcagga accgccggag ttcgacgtca gactgcgctc cgtgctgctg
     1921 cggacctcgg ccatggtctg ggagacctac agggagttcc gccgcagacg agggctgttg
     1981 tcgtacgacg acatgatccg tctggcggct gatatcgccg cgtccgaaaa ttcggcgcgc
     2041 tccgtgcgca cgttccgcga gatcctcgtg gacgagtatc aggatacgaa cccgctgcag
     2101 gaccgtctga tcgactccgt cgccgccgaa gggtgcaggc gctttcttgt cggcgacccg
     2161 aagcagtcga tctaccgctt ccgccacgcc gacccgacgc tgttcggaga acgtatcgcc
     2221 tcgccgtccg aggagacgcg ctacatcccg ctgcaaacga gcttccgcac ccgccccgcg
     2281 ctgttgaagg agatcaacgg actcttcggc ggcatctggc ggaacggcat cgcctccacg
     2341 cttcccaccc cctacgagga gctgctcttc ccggagaatc ccgagatgcg ggagctgcgc
     2401 gaggagacgt ccctccctcc gctccttccg gtctttcgcc gtcagcggga aaacgaaccg
     2461 gtcgcggaga ctcgaaaacg ggtggcgctt gctctcggag agaagctgtt ggagctgcac
     2521 ggccagcccg tctggaacaa aaagaacaaa cagatgctcc ctgctgagtg gcgcgacatg
     2581 accattctcg tgccgtcgcg ctcctccttc gccgcgctcg aggagacgct gcaccctctt
     2641 ttcggcatcc ccgtcgcctt cgagaaggga aagcgctact tcgacagggg agagatcggc
     2701 gatctgacga ctgccatccg gatgctcgcg ttccccgacg acagggcggc cgcgctcggt
     2761 tttctggcct cccccttctc cgggctgaac atggagcagt tcgcccgctt cctcgggccg
     2821 gaggccccct cgcttgcgga ggtctttccg gagacggcgg ccaggatcga ggagctgcgg
     2881 agcgtcgcgc gctactccgg cctcttc
//
LOCUS       DAOSWW010000268         2907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25484_length_2907_cov_3.679523, whole genome shotgun sequence.
ACCESSION   DAOSWW010000268 DAOSWW010000000
VERSION     DAOSWW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25484_length_2907_cov_3.679523"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PK442_06330"
     CDS             complement(<1..537)
                     /locus_tag="PK442_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO87302.1"
                     /translation="MRNNVGEEIVHRLAVSGQEFSLYIHLPFCARKCPYCSFFSVVPR
                     NGEIEEYCRLLHSEIRFFGERLAGSGAPAGTIYFGGGTPSLLAPADWERLFKCLRKHI
                     LFDHKVEISVEANPDSLSESHVRLWKEQGVSRISVGVQSFIDGELRWLGRLHDADRAR
                     MALAMCVHSGISVSADFMF"
     gene            complement(527..2335)
                     /gene="lepA"
                     /locus_tag="PK442_06335"
     CDS             complement(527..2335)
                     /gene="lepA"
                     /locus_tag="PK442_06335"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOO87303.1"
                     /translation="MEQKRIRNFCIIAHIDHGKSTLADRLLELTQTVESRDMKHQMLD
                     TLDLERERGITIKLVPVRMKYRAEDGEDYILNLIDTPGHVDFGYEVSRSLAACEGALL
                     VVDATQGVEAQTLANAYMAVDQGLEILPVINKIDLPSSRPDYVIKEIEDVVGLDCDKV
                     ILASAKEGKGVPEILERIVHGVPAPSGNPEAPLQAMIFDSKYDNYKGVICYIRVMNGT
                     LRPGRNIRFMATGGVFPLEEVGVLRPNFEPCDELTAGEVGYIVSNIKTVAEAHVGDTI
                     TEDARPATAALPGYRKVKPVVFCGFYPVERDDYPQLRDALEKLTLNDSSISFEPETSL
                     ALGFGFRCGFLGLLHMDVSKERLRREYSVELVATSPNVIYELTTKSGEIIEAHRPSDF
                     PEIGDIEEVREPMIKLTLFVPSDYVGKVMKLCQDKRGTYISMDYLAPDRVRLIYEVPL
                     AEFIIDFHDKLKSHTRGYASLDYEHTGLKASDLVRVDVLINGEAADAFSFICHREAAY
                     NRGQAVVRKLKELIPSQLFEVPLQASIGKKVIVRQNVKPLRKDVLAKCYGGDITRKRK
                     LLEKQKEGKKRMKQIGRVSIPQEAFLAFLKVEEDEE"
     gene            complement(2387..>2907)
                     /locus_tag="PK442_06340"
     CDS             complement(2387..>2907)
                     /locus_tag="PK442_06340"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOO87304.1"
                     /translation="DILLSVAPRQVSYVSTVLSGIISLYGIRTVEDRLPVEKSPSEVD
                     EKRSGPLAGIVAGLEACSSEWLFVCACDMPRVSEAVVRTLWKHGDKSASVIVPFLGGF
                     YEPLHAFYRKTCLPAATALLDSGRRKTTAFYDAVRVSPVGDEYFSHLPGYRRSFENLN
                     NKSDLEKMTGFL"
BASE COUNT          687 a          813 c          740 g          667 t
ORIGIN      
        1 gaacataaaa tctgcgctca ccgaaatgcc tgagtgaacg cacattgcca acgccatcct
       61 ggcgcggtcc gcgtcatgga gtcgtccaag ccagcgaagt tcgccgtcaa taaagctctg
      121 cacaccgacg ctgattctgg agacaccctg ctctttccag agacgcacgt gactttcaga
      181 aagggagtcg ggatttgcct ccacgctgat ctctaccttg tgatcgaaaa ggatatgctt
      241 gcgtaaacat ttgaaaagac gctcccaatc agccggagcg agcagcgacg gggtgccacc
      301 tccgaagtaa atagtccccg cgggtgctcc gcttcccgca agacgctctc cgaaaaaacg
      361 gatctccgaa tgcagaagcc tgcagtactc ttctatttcc ccgtttcgcg gaaccacgct
      421 gaaaaacgaa cagtagggac atttccttgc acagaacggc agatgaatat aaagagaaaa
      481 ctcctgtccg gagacggcga gccggtgaac aatctcctcg ccgacgttat tcctcatcct
      541 cttctacctt cagaaatgcg agaaaagctt cctgggggat agacacgcgc ccaatctgct
      601 tcatgcgctt ctttccctct ttctgctttt caaggagttt ccgttttcgg gtaatgtcgc
      661 ctccgtagca cttcgcaagc acgtcttttc gaagcggctt tacattctgt cgaacaatga
      721 ctttttttcc tatggacgcc tgaagaggca cttcaaaaag ttggctcgga atcagttcct
      781 tgagttttcg caccacagcc tggccccgat tgtaggcggc ttccctgtgg cagatgaagg
      841 agaacgcatc ggcggcttct ccgttgatca gcacatcgac acgaaccagg tcggacgcct
      901 tcagaccggt atgctcgtag tcaagggacg cataccccct tgtatgggac ttcagcttgt
      961 cgtggaaatc aatgataaac tccgccaacg gcacttcata aatcaagcgt acacggtccg
     1021 gggcaagata gtccatggag atatacgttc cccgtttatc ctgacagagc ttcatcacct
     1081 ttccaacata gtcggacgga acgaagagag tcagtttgat catcggctcc cgcacttctt
     1141 cgatatcacc gatctcagga aaatcggagg gacggtgggc ttcaatgatc tcgccgctct
     1201 tcgtcgtcaa ttcatatatg acgttcggtg aagtggcgac gagctccacg ctgtactcgc
     1261 ggcgaagacg ttcctttgac acatccatgt ggagaaggcc gagaaacccg caacggaacc
     1321 cgaatccgag agcaagcgaa gtctccggtt caaaggagat cgaggagtcg ttaagcgtca
     1381 gcttttcaag agcgtcacga agctgagggt agtcgtcgcg ttccacggga tagaagccgc
     1441 aaaaaacgac aggtttgacc tttctgtagc cggggagcgc cgccgtcgcc ggacgcgcat
     1501 cctccgtgat ggtgtctccc acgtgcgctt ctgccacggt cttgatgttc gatacgatat
     1561 agccaacctc tcccgccgtc agttcgtcgc acggttcgaa gttcgggcgc agcacgccga
     1621 cttcctccag gggaaaaacg ccgccggtcg ccataaaccg gatattgcgg ccggggcgca
     1681 gcgttccatt cataacgcgt atgtaacaga taacgccttt gtaattatcg tactttgagt
     1741 cgaagatcat cgcctgcaac ggcgcttcag gattgcccga cggcgcgggt actccgtgaa
     1801 cgatgcgctc cagtatctcg ggaactcctt tgccctcctt ggcgcttgcg agtatcacct
     1861 tgtcgcagtc aagcccgaca acatcttcta tttccttaat tacatagtcg gggcgggatg
     1921 acggaaggtc gatcttgttg atcaccggga gaatctccaa cccttgatca accgccatat
     1981 acgcgttggc gagagtctgc gcctcaacgc cctgtgtggc gtcgacaacg agcagcgctc
     2041 cttcacaggc ggcaagagag cgggagacct catatccgaa gtccacatgc cccggagtat
     2101 cgatcaggtt gagaatatag tcttctccgt cttcggctcg atatttcatg cgtaccggaa
     2161 caagcttgat ggtgatcccc cgttctcttt caagatcgag ggtatcaagc atctggtgtt
     2221 tcatgtcccg tgattcaacg gtttgcgtca attccagcag tctgtcggca agggtagatt
     2281 ttccatgatc aatatgggcg atgatgcaga agttgcgaat gcgtttctgt tccatacaac
     2341 gtctccttcc aaaaatggcg gtgcttcagt ctcaatactg gctcctttaa aggaaacctg
     2401 tcattttttc cagatcagat ttgttgttga ggttttcaaa agatctcctg taaccgggaa
     2461 gatgagagaa gtattcatct cccaccggag acactctaac ggcgtcataa aacgccgtgg
     2521 tctttctccg tccgctatcc agaagcgctg tcgcggcggg caggcatgtt ttcctgtaga
     2581 aagcatgaag cggttcataa aagcccccga ggaacggaac aattaccgaa gcgctcttgt
     2641 cgccgtgttt ccagagcgtt cgtacaacgg cctccgagac tcgcggcata tcacaggcgc
     2701 agacaaaaag ccattcggaa gaacacgcct ccaacccggc aacgatgcct gccagcgggc
     2761 cgcttctctt ttcatcaacc tccgaagggc ttttctcgac aggaaggcga tcctcgacgg
     2821 tacgaatgcc gtacagagag attatacccg acaacacagt ggaaacatac gaaacttggc
     2881 ggggggcgac tgaaagcagg atgtcct
//
LOCUS       DAOSWW010000269         2901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25567_length_2901_cov_4.864371, whole genome shotgun sequence.
ACCESSION   DAOSWW010000269 DAOSWW010000000
VERSION     DAOSWW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25567_length_2901_cov_4.864371"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PK442_06345"
     CDS             <1..1373
                     /locus_tag="PK442_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOO87305.1"
                     /translation="HYLLHPDKNAHLPEAVIPGYESAALAERGAAILAEYGRMERELA
                     KYPGLSDLMRTVDIPLIPVLVDMEQYGISCCSQTFGALEKDLSERLSGIEEEIASTAG
                     ERINLNSPKQVAWLLFEKLGLPAGKKTKTGYSTDISVLEGLSALGRPFDEVPNLLIEY
                     RELSKMLSGFVQPLTKAGEAGEGVIHGIFEPAVTGTGRLSSREPNLQNLPTFGEWSKR
                     VKEGLQPHGEDRMFVAADYSQIELRILAHLCGDKRLLDAFASGRDIHTETASWVFSTE
                     PAFVTPELRRVAKMINFGLLYGMSAFGLAQRLAIGRQEASDIVKRYFEALPGVKEYLD
                     AGYIEAKERGYTRTVAGRIRPLAEVSVSPRDRDALRRVAVNTPIQGSAADIARKAMVD
                     FARRFPKEGSVRLALQVHDSLVCECARDEAPAVESELVKIMEGAATLSVPLKVEAKKG
                     GNLAEV"
     gene            1449..2555
                     /locus_tag="PK442_06350"
     CDS             1449..2555
                     /locus_tag="PK442_06350"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOO87306.1"
                     /translation="MRTLRSQVRWIMIAIVVLFVLSIFGMYGFDGARRGRPQSGESGD
                     YVVAEIDGKPVMRSALDQSVRNYVERMNVREIKPEDIPHLYQSTLESLVINHRLALEA
                     EQSGLKATDEELDAAVREVSEQFPTKEAFMQYLDQSGVKMAAFRENLATQIVQQKMVE
                     RASAGIAVTEEEALDFYEKTKVLFFHSPAGYTMDYARLKNEAAAAKIVEAVRQGAEWN
                     AVWSSVTSDDLVERTPESGPVFVPETAFKEQLAAFADLEPGTIGGPAQIASDDFFVGV
                     KREKVEESTVAYNEVSGDVKALLTEEKRREAQTSFFKELRGKASVIIHDFDLFPKPEA
                     PKDAAGVPVSADKAPASGPVSADQAPVSGDAEKK"
     gene            2623..2717
                     /locus_tag="PK442_06355"
     tRNA            2623..2717
                     /locus_tag="PK442_06355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2658..2660,aa:Ser,seq:gct)
     gene            2821..2897
                     /locus_tag="PK442_06360"
     tRNA            2821..2897
                     /locus_tag="PK442_06360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2855..2857,aa:Arg,seq:acg)
BASE COUNT          595 a          802 c          939 g          565 t
ORIGIN      
        1 cgcactacct gctgcacccc gacaagaacg cgcacctccc ggaggcggtc atccccggct
       61 atgaatcggc ggcgcttgcg gagaggggag cggcgatcct tgcggaatac ggcagaatgg
      121 aaagggagct ggcgaagtat cccggtttgt ccgacctgat gcgcaccgtg gatattccgc
      181 tgatccccgt gcttgtggat atggagcagt acggcatctc ctgctgttcg cagacgttcg
      241 gcgcgctgga aaaggatctt tcggagcgcc tctccggcat cgaggaggag attgcgtcga
      301 cggcggggga gcggatcaac ctgaattccc cgaaacaggt ggcgtggctg ctcttcgaga
      361 agctcggcct tcccgccggg aaaaagacca agacggggta ctcgacggat atctccgtcc
      421 tggaggggct ttccgcactc ggtcggccct tcgacgaggt gccgaacctc ctcatagagt
      481 acagggagct ctcgaagatg ctctccggtt ttgttcagcc gctgacgaaa gccggcgagg
      541 cgggggaggg cgtgattcac ggcatcttcg agcccgcggt gaccggcacc ggaaggttga
      601 gcagcaggga accgaacctc cagaaccttc ccacgttcgg cgaatggtcg aagagggtga
      661 aggaggggct tcagcctcac ggagaggatc gaatgttcgt cgccgcggac tattcgcaga
      721 tcgaactccg catcctcgcg cacctctgcg gcgataaacg gttgctggac gccttcgcct
      781 cgggacggga cattcatacc gagacggctt cgtgggtctt ctccaccgag cccgcgtttg
      841 taacgccgga actgcgccgc gtcgccaaga tgatcaactt cggtctgctc tacggaatga
      901 gcgcgttcgg ccttgcgcag cgcctcgcca tagggcggca ggaggcttcg gacatcgtca
      961 aacgctactt cgaggcgctt cccggagtca aggagtacct cgacgcagga tatatcgagg
     1021 cgaaggagcg agggtacacc cgtaccgtcg ccgggcgtat ccgtcctctg gccgaggtct
     1081 ccgtcagtcc tcgcgaccgg gacgccttgc ggagagtcgc ggtgaacacc ccgatccagg
     1141 ggagcgccgc cgatattgcg cgcaaagcga tggtcgactt cgcgcgccgc tttccgaagg
     1201 aggggagcgt acggctggcg ctccaggtgc acgactcgct cgtctgcgaa tgcgcgcgtg
     1261 acgaagcgcc tgcggtagag tcggagcttg taaaaatcat ggagggcgcg gccactctct
     1321 ccgttcccct caaagtcgag gcgaaaaaag gaggaaatct cgccgaggtg taagcgaaaa
     1381 cgttcttctg tggtacaatt ctcccttgga gtttggatat tcgaaagaga ggaagatgtc
     1441 cgttgttgat gagaaccctg agatcccagg tgcggtggat catgattgca atcgtggtat
     1501 tgttcgtgct ctctattttt ggtatgtacg gttttgacgg agcccggaga gggcgccccc
     1561 agagcggtga aagcggagat tacgttgtgg ctgagatcga cggcaagccg gtgatgcgtt
     1621 ccgcgctcga tcagagcgtc aggaactacg tggagaggat gaacgtccgg gagatcaagc
     1681 cggaggatat tccgcacctc taccagtcga cgctggaaag cctcgtgatc aatcatcgcc
     1741 tggcgctgga ggcggagcag tccggcctga aggcgacgga tgaagagctt gacgccgctg
     1801 tcagggaagt aagcgagcag ttcccgacca aggaggcttt catgcagtac ctcgaccagt
     1861 caggggtaaa aatggccgct ttccgcgaga accttgccac gcagattgtc cagcagaaga
     1921 tggtggagcg cgcttccgcc ggtatcgccg tgacggagga ggaggcgctc gacttttatg
     1981 aaaagacgaa agtgctcttc ttccactctc ccgcagggta cacgatggac tacgcgaggc
     2041 tgaagaacga agccgccgcc gcgaagatcg tcgaagccgt ccgccagggg gccgagtgga
     2101 acgccgtgtg gagttcggtg acatcggacg atctggtgga gcgcacgccg gagtcaggtc
     2161 ctgtgtttgt tcccgaaacc gcttttaaag aacagctcgc cgccttcgcc gatctcgaac
     2221 cgggtacgat cggcggtccg gcgcaaatcg ccagcgacga cttttttgtc ggggtcaagc
     2281 gtgaaaaggt cgaggagtcg acggtcgcct acaacgaggt gagcggcgat gtgaaggcgc
     2341 ttcttacaga ggagaagcgc agggaagcgc agacctcttt cttcaaggag cttcgcggga
     2401 aggcgtccgt gattatccac gacttcgatc tcttccccaa gccggaggct cccaaggatg
     2461 ccgcgggcgt tcctgtatcc gcagataaag cgcctgcgag cgggcctgtt tccgccgacc
     2521 aggcgcctgt gagcggagat gcggagaaga aataatcgtc ttttcgtgct ttgcctattg
     2581 acataaattc cggcctttta tagaatagag cgcgctgttt gtggtgggtt ggccgagtag
     2641 gtcgaaggcg ctcgcctgct aagcgagtag gggggcttaa acctccctcg agggttcgaa
     2701 tccctcaccc accgccactt acacgcagcg tttcttttct ccggggatat tgccaagcgc
     2761 aaaagaaagt gctaaaatcc tccgggtgtt cagaggagca gggttccttt gaacaagtac
     2821 gcgccggtag ctcagatgga tagagcggcg gtctacggaa ccgcaggtca cgagttcgaa
     2881 tcttgtccgg cgcgccattt t
//
LOCUS       DAOSWW010000270         2896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25644_length_2896_cov_3.285463, whole genome shotgun sequence.
ACCESSION   DAOSWW010000270 DAOSWW010000000
VERSION     DAOSWW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25644_length_2896_cov_3.285463"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK442_06365"
     CDS             <1..487
                     /locus_tag="PK442_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO87307.1"
                     /translation="LLFVRTPELFSIVLAGGFIASVAMLILGMTVVPRLSKIVLVPKR
                     ILMPLVTVLCVVGTYAVNSSMFDVGLMFCFGLAGFLMRSRGYPPAPLVLGLVLGEMMD
                     SNLRRAINMALAGDNFFLSLFGRPTSVILAVLVILSVLAAVPGVKDRVLSVFSRRERV
                     E"
     gene            complement(528..1820)
                     /locus_tag="PK442_06370"
     CDS             complement(528..1820)
                     /locus_tag="PK442_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO87308.1"
                     /translation="MIQRHFKDVCCDILILGGGIAGAAAALAAAREGASVVLAEKSGR
                     LGGIATNGLVSTLCGCYSSLPGRRKIIGGFRDLLAAELHRTGGILEGVDRDTHRNDTC
                     DPELLACALDTLMQRHDVQLLFSSCVIGADFSEPLLRSVEVVNTESGTRCSITPRLAV
                     DASGGGLLSRLADPASIVSGGEGGKLQAATMVFRMDGVDGDRARLVTKDEIRALLAEA
                     RRNGDVVGERGNGVLTVLPGNSSAIVNANWVQANMSVPEDVTRGLVEGREKALENARF
                     FRKYVPGFGNAVLSSIAASLGVREASRIKGKYVLSQEDVMEGRRFEDAISLGSWPMEY
                     HDYEQNRLVYLWSENDYDIPFRCLLPEGLDNVIAAGRNISVSSMAYASTRVMGPCMAI
                     GQAAGTATRLCLKMNALPKEVDIHLLRKTLLNEGAILE"
     gene            2185..>2896
                     /locus_tag="PK442_06375"
     CDS             2185..>2896
                     /locus_tag="PK442_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO87309.1"
                     /translation="MISTSRRMELEKELLEQFHLQEQIFSLVPVPLVMKDANSVYLHV
                     NQAFADFLGLPREQVLGKGPFDLFSPEFAQRIFEEDQEIIRNGVPATDLERRIKDGHG
                     KMVWLKSYKGPVFNSSGGVIGIVGGNMDITAVKNAEEALRKSEERWKFAVEGAGDGVS
                     EWNLETGDVFYSKQWKAMLGYKDDEIAPTFEEQKKRIHPDDLSEVMFALEQHLRGETP
                     SFSHEFRLATKNGGWIWVL"
BASE COUNT          598 a          813 c          845 g          640 t
ORIGIN      
        1 gctgctcttt gtccggacgc ctgaactctt ctccatcgtc ctcgcagggg gatttatcgc
       61 ttccgtcgcc atgctgattc tcgggatgac cgttgtcccg cggctctcga agatcgtgct
      121 cgtcccgaag agaatcctga tgccgcttgt gacggtgctc tgcgtggtcg ggacctatgc
      181 ggtgaactcc agcatgttcg acgtgggatt gatgttctgc ttcggactgg cggggtttct
      241 gatgcggagc cgggggtatc cgcccgcgcc gcttgtcctc ggtctcgtgc tgggggagat
      301 gatggactcg aatcttcggc gtgcgatcaa tatggcgttg gcgggggata actttttcct
      361 ctcgctcttc ggcagaccga caagcgtgat cctcgcggtt ttagtgattc tctccgttct
      421 cgccgccgtg ccgggagtga aggaccgggt gctctcagtt ttttcaagaa gagagcgggt
      481 agaataaagc agaataatga aggggaggcc gaccagcctc cccttcatta ttccagaatc
      541 gccccctcgt tcagaagcgt ctttcggagc aggtgtatgt ccacttcctt ggggagcgca
      601 ttcatcttca ggcagaggcg cgtcgccgtt cccgcagcct ggccgatggc catgcacggt
      661 cccatcactc gggtggaggc gtatgccatc gacgataccg agatgttccg ccctgctgcg
      721 atgacgttgt ccagcccctc ggggagcaga cagcggaacg gaatgtcgta atcgttttcg
      781 ctccacaggt agacgagcct gttctgttcg tagtcatgat actccatggg ccaggagccc
      841 agcgaaattg cgtcttcgaa gcgccgcccc tccatgacgt cctcctgcga gaggacgtac
      901 ttgcccttga tacgcgacgc ctcgcgcacg ccaagtgaag ccgcgatgga ggagaggacc
      961 gcgttcccga agcccgggac gtacttccgg aagaaacggg cgttctcaag cgccttttcc
     1021 cggccttcca caagcccgcg cgtcacatct tccggaaccg acatattggc ttgcacccag
     1081 ttggcgttga cgatggccga gctgttgccg ggaagcactg tcaagacgcc gttgccgcgc
     1141 tccccgacga cgtcgccgtt ccggcgggcc tcggcgagca gcgcgcgtat ctcgtccttg
     1201 gtgacgagac gagccctgtc tccgtcgacg ccgtccatgc ggaagaccat ggttgcagcc
     1261 tggagtttgc ctccttcgcc gcccgagacg atggatgccg ggtcggccag ccgggagagg
     1321 aggcctcctc cggaggcgtc gacggccaga cggggagtta tcgaacaccg cgtccccgat
     1381 tccgtgttga cgacctcgac ggaacgaaga aggggttccg agaagtccgc gccgatgacg
     1441 catgaggaga acaacagctg cacatcgtga cgctgcatca gagtgtcgag ggcgcacgcg
     1501 agcaattccg gatcgcaggt gtcgtttctg tgcgtgtctc tgtcaacccc ttccagtatg
     1561 cctccggtac ggtgaagttc cgccgccagg agatcgcgga atccgccgat gattttgcgc
     1621 cttcccggca gtgaagaata gcagccgcag agcgtgctca caagaccgtt cgtcgcgatg
     1681 ccgccaagtc gcccgctctt ttccgccagg acgaccgacg ctccctcgcg ggctgctgcc
     1741 aaagctgccg ccgcacctgc gatgccgcct cccaggataa gaatatcgca gcaaacgtct
     1801 ttgaaatggc gttgaatcat tatggcctcc tcatttcttc gcttccgaac agttttacag
     1861 gaagcggcga ttcttcagtc gagaggttaa gtgtaacgga tacgacgtac gcgaagcaat
     1921 ctcagaaaac ttttttcaga gaatcaggca aattattttt ttgtttcccc ctttgtgata
     1981 gtattgtttc attcacgcga ctccgcagac ttgagaaatc ccgagaaata cccgggtttt
     2041 ttgcatcgtt tccacacaca agggggaaat tattcagtgt cctgcaaaaa aagtaccaca
     2101 ccggacatgg tgcttcaaaa aaaaggaccg gctcctcttc gtcagaagac aaaaaaggaa
     2161 ttcgagtctt tatccggaga acctttgatt tcaacgtctc gccggatgga gctggagaag
     2221 gagctattgg agcagttcca tctgcaggaa caaatttttt ccctggttcc cgttcccctg
     2281 gtcatgaaag atgcgaattc cgtctatctc catgtcaatc aggcgttcgc cgattttctt
     2341 gggcttccca gggaacaggt gcttggtaaa ggacccttcg atcttttttc tcccgagttc
     2401 gcgcaacgca ttttcgaaga ggatcaggaa atcatacgaa acggcgttcc cgcgaccgat
     2461 ctggaacgcc gtattaagga cggccacgga aagatggtat ggctgaaatc gtataaaggc
     2521 cctgtgttca acagcagcgg cggcgtgatc ggtatcgtcg gaggcaacat ggacattacg
     2581 gcggtcaaga atgctgaaga ggcgctccgc aagagcgaag agcgatggaa gttcgccgtc
     2641 gagggggcgg gcgacggagt cagcgagtgg aatctggaga ccggcgatgt gttttattca
     2701 aaacagtgga aagcaatgct cgggtataag gatgacgaga ttgcgccgac cttcgaggaa
     2761 cagaaaaagc gcatacaccc cgacgacctc agtgaggtga tgttcgcgct ggaacagcat
     2821 ctgcgggggg aaaccccttc tttttcccac gaattccgtt tggccacgaa aaacggcggc
     2881 tggatttggg tcctcg
//
LOCUS       DAOSWW010000271         2895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25657_length_2895_cov_4.681338, whole genome shotgun sequence.
ACCESSION   DAOSWW010000271 DAOSWW010000000
VERSION     DAOSWW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25657_length_2895_cov_4.681338"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK442_06380"
     CDS             <1..372
                     /locus_tag="PK442_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOO87310.1"
                     /translation="SDPKSVAAKFNEGYGFNSVIDAAGPAATLKLSMELVMPAGIINK
                     VAWGPKPVDFSLDPLLSKGATLQGSFSHSWDIWEKVLVIMGQGQMPLDALITHELPLE
                     GWKEGFDLIESRKGLKVVLKP"
     gene            complement(535..1428)
                     /gene="otsB"
                     /locus_tag="PK442_06385"
     CDS             complement(535..1428)
                     /gene="otsB"
                     /locus_tag="PK442_06385"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOO87311.1"
                     /translation="MKKKKIDSPAGAGFNAPPGNGERCAAEEILQTGDPLPSALDGFD
                     AIARKAEGKRIAIFLDYDGTLSPIVETPEQAIMAADMREAVVKLAECTPLGIISGRDL
                     EDVRDKVKIDGIVYAGSHGFDITGPDGLKFENTVGEDFLPALDSAENALSEKIGAIEG
                     LFLERKKFAIAIHYRRVAPENIERIEAAVDETTSDHPDLRKAYGKKIFELQPKADWHK
                     GKALFSLMRTLKLDRDDVLLVFIGDDATDEDAFRALQGRGVGIVVREKAYETAAAYSL
                     KDPDEVREFLLRLIPLCRGSS"
     gene            complement(1475..>2895)
                     /locus_tag="PK442_06390"
     CDS             complement(1475..>2895)
                     /locus_tag="PK442_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOO87312.1"
                     /translation="RIDVVPFDSANKFMATLDRLPGGGVRILFKGAPDRLLERCGQQR
                     GADGTVQPLDRESWEQRMEELGGEGLRVLAAAFKDADEGKSGLDMEDVENDMVFLGLV
                     GIIDPPRPEAIEAIKICRDAGIKVKMITGDHAGTASAIGREMGITGTGLAVTGEELEK
                     ASDEELRTIVRDADIFARTSPEHKLRLVQALQANGEVVAMTGDGVNDAPALKRADVGV
                     AMGIKGTEATKEAADIVLADDNFISIERAVEEGRTIYDNLRKAILFILPTNGAEALVI
                     LAAVVFGLSLPLTPVQILWVNMVTAVTLALALAFEPAEPGVMQRPPRSPDAPILGGLF
                     LWRIAFVSVLIGGATIAVFLVERRLGMPLETARTLAVNTLVCGQAFYLFNSRYLRESS
                     IAPDRVFANRAAWIAVGVLAVLQIVFVYAPFMQLWFGSAPLELRHWLIPLSLGAAVFI
                     VVEAEKNVTRRVRAANGRRSGV"
BASE COUNT          533 a          955 c          847 g          560 t
ORIGIN      
        1 tcggacccga agagcgtggc cgcgaagttc aacgaggggt acggcttcaa ctccgtcatc
       61 gacgcggcag gacccgccgc gacgctgaag ctctcgatgg agctcgttat gcccgcagga
      121 atcatcaaca aggtagcgtg ggggccgaaa cccgtggatt tcagcctcga cccgctgctc
      181 tcgaagggcg cgacactcca ggggtccttc agccactcgt gggacatctg ggagaaggtg
      241 ctcgtcatca tgggacaggg gcagatgccg ctcgacgccc tcatcaccca cgaactcccg
      301 ctggaggggt ggaaagaagg attcgacctc atcgaaagcc gcaaagggct gaaggtcgtg
      361 ctcaagccgt agcggctgaa aaagctattt ttttggaggg agggcgttcg cacgtcctcc
      421 ctttcaaaat tcaagagagt ccattcgcag gatatcgctg ttccgtgttt ccgacgattt
      481 tccgaacaag gccccgaaga caatggctct tctgtgcgag cacatcccac gccatcatga
      541 cgaccccctg cacaacggaa tcaaacggag aagaaactcc cggacttcat cgggatcctt
      601 caggctgtat gcagcggcgg tctcgtaggc tttttcccgg accacgatgc cgacgcctct
      661 tccctgcaac gcgcggaacg cgtcctcgtc ggtcgcgtcg tctccgataa agacaaggag
      721 cacgtcgtcc cggtcgagtt tcaacgtgcg catcagcgag aagagcgcct tccccttgtg
      781 ccagtccgcc ttcggctgga gctcgaagat cttcttgccg taggctttcc tgagatcggg
      841 gtggtccgac gtcgtttcgt cgacggcggc ctctatccgc tcgatgtttt ctggagcaac
      901 ccgccggtag tggatggcga tggcgaactt ttttctctcc aaaaaaagcc cttcaatggc
      961 gccgatcttc tccgacaggg cgttctccgc gctgtcgagc gcggggagaa aatcctcccc
     1021 tacagtattt tcgaacttta gtccgtcggg gcccgtgatg tcgaaaccgt ggcttcccgc
     1081 gtagacgatg ccgtcgatct tcaccttgtc gcgcacatct tcgagatccc ggccgctgat
     1141 gatcccgagc ggagtacatt cggcgagctt gacgaccgcc tcccgcatat ccgcggccat
     1201 aatcgcctgc tccggagtct ccacgatcgg agagagcgtg ccgtcgtaat caaggaagat
     1261 cgcaatccgc tttccctccg ccttgcgggc gatcgcgtcg aaaccgtcca gagcggaggg
     1321 aagcggatcg ccggtttgaa ggatctcctc ggccgcgcac ctctctccgt tccccggtgg
     1381 cgcgttgaaa ccagcccccg ccggggagtc gattttcttt tttttcattt ctatacctcc
     1441 gcttcttcga tatctcttcc aacgcctgtt cggactacac gccgctccgg cgtccgttcg
     1501 cggcgcgcac acggcgcgtc acgttctttt ccgcttcgac gacgatgaag accgccgcgc
     1561 ccagagagag gggaatcagc cagtggcgaa gctccagagg cgccgagccg aaccagagct
     1621 gcataaacgg agcgtagacg aagacgatct ggagtacggc cagcacgcct accgctatcc
     1681 acgcggcgcg gtttgcgaaa accctgtcgg gcgcaatact cgactcccgg agataccggc
     1741 tgttgaaaag atagaacgcc tgtccgcaga cgagcgtatt gaccgcgagc gtccgcgccg
     1801 tctccagcgg cattccgagc cggcgttcca caaggaagac ggcgattgtc gcccccccga
     1861 tcagcacgga gacgaacgcg atgcgccaca ggaagagtcc gccgagaatc ggggcgtcgg
     1921 gcgagcgcgg agggcgctgc atcactcccg gctcggccgg ctcgaaggca agagcgaggg
     1981 cgagcgtgac ggcggtcacc atgttgaccc agaggatctg cacgggcgtc agcggaaggg
     2041 acagcccgaa gaccacggct gcaagaatga ccagcgcctc ggcgccgttc gtcggcagga
     2101 tgaagagaat ggccttccgc agattgtcgt agatggtgcg cccctcctcc accgcgcgct
     2161 cgatggagat gaagttgtcg tccgccagca cgatgtccgc cgcctccttg gtcgcctccg
     2221 tccctttgat ccccatggcc acgccgacgt cggcccgctt gagcgcggga gcgtcgttca
     2281 cgccgtcgcc ggtcatcgcg acaacttcgc cgttcgcctg aagcgcctgc acaagccgca
     2341 gcttgtgctc cgggctggtg cgggcaaaga tgtccgcgtc ccgtacgatc gtgcgcagct
     2401 cttcgtcgga agccttctcc agttcctcgc cggtaacggc aaggcctgta ccggtaatgc
     2461 ccatctcgcg tccgatggcg cttgcggtcc ccgcatggtc gccggtgatc atcttcacct
     2521 tgattcccgc gtccctgcat atcttgatgg cctctatcgc ctccggacga ggcgggtcga
     2581 taatgccgac gaggccaagg aacaccatgt cgttttcgac atcctccata tccagcccgc
     2641 tctttccctc gtccgcatct ttgaaggcgg cggcaagcac gcgaagccct tcgccgccca
     2701 gctcctccat ccgctgttcc cacgactccc ggtccagcgg ctgaacagtt ccgtccgcgc
     2761 ccctctgctg tccgcacctt tccagcagcc tatccggggc gcccttgaac aggatcctga
     2821 cgcctcctcc gggaagccgg tcgagcgtcg ccatgaactt gttggccgag tcgaacggca
     2881 cgacgtcgat gcggc
//
LOCUS       DAOSWW010000272         2882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25876_length_2882_cov_3.476477, whole genome shotgun sequence.
ACCESSION   DAOSWW010000272 DAOSWW010000000
VERSION     DAOSWW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25876_length_2882_cov_3.476477"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..>706)
                     /locus_tag="PK442_06395"
     CDS             complement(237..>706)
                     /locus_tag="PK442_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOO87313.1"
                     /translation="NARESLWLCTPYLIPGKTLSTALTVASMSGVDVRVIIPHAADHA
                     MVHWASLSSVEELLRAGVKVYSYRKGFHHAKIMIADDHTVSVGSANFDARSLEINFEV
                     QAFLYDKKVAKQFRREFLNDLQECSQFRLHDWLRRSLRSRILESAGRLWSSQL"
     assembly_gap    707..716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<717..1667)
                     /locus_tag="PK442_06400"
     CDS             complement(<717..1667)
                     /locus_tag="PK442_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOO87314.1"
                     /translation="MNFLKEYSFSFLWAYGIFIAGIIFLEERNPDRTIAWLLVLLLLP
                     VIGFLIYFFFGPDLRERGNFRKIKKKRRKNRKGPLFVSHEHNEPCAFLGEHTKKTASL
                     LTKSAKARVSTHNSVEMLVNGEATFASIKHALRQAKRYIHLEYYSIDNDAIGGEIRDI
                     LLEKAAEGVDVRLIYDSVGSWKLGKKFIDTLKHGGVHVHSFLSVSFPMLRRDLNFRNH
                     RKIIVIDGITGYLGGLNIGDSYIHGEPGLGIWRDTHLKIEGEAVACLDEIFAADWTFC
                     DLKAPPAHDIPNAVHNEITPVQIVSSGPDSNWKSILHGYFS"
     gene            1957..2040
                     /locus_tag="PK442_06405"
     tRNA            1957..2040
                     /locus_tag="PK442_06405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1990..1992,aa:Leu,seq:tag)
     gene            complement(2045..2611)
                     /locus_tag="PK442_06410"
     CDS             complement(2045..2611)
                     /locus_tag="PK442_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO87315.1"
                     /translation="MNLVLETIKKRRSVRSFRPDQITDDELNSIIESGLWAPSGHNTQ
                     PWHFLVVQDKSKIDQMSRSTIEKMANSPIDWVRKLAAKEGYHLFHGAPTVIIVSGRQS
                     GDTFLPVVADCAAAIQNMLLGAESLNIGTCWIGLTSFLFEDSEETASLKIPEGYAPYY
                     SIAVGYKSAGFIPAPPTRKEGTVSTFGK"
     gene            complement(2625..>2882)
                     /locus_tag="PK442_06415"
     CDS             complement(2625..>2882)
                     /locus_tag="PK442_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87316.1"
                     /translation="SDAQHRMVFSARREGCCKSWSRLEKDGKDQGYQETKNGIISDSH
                     SVAAPFCREKTAAEGEKENAQWHFPHFWDIVAYTHRQKHFF"
BASE COUNT          665 a          702 c          710 g          795 t
ORIGIN      
        1 gcagctgact cttaatcagc gggtcgtggg ttcgagtccc tcacagtcca ccattaaaat
       61 aaaagtgcga aaagctttta aaagcttttc gcacttttat tttttgctct gacaggtcat
      121 cgtattgatc ggatgcgccc tgtacttttt ttattcaacc gccgttttct taagagagac
      181 gttctcttca atcctctctt gtcctgcgtt cctgcgatct ttgtttcgct ttcggtttat
      241 aattgcgagg accacaggcg ccctgccgat tccagaatgc ggcttctcag ggagcggcga
      301 agccagtcgt gcagtcgaaa ctgggagcac tcttgaaggt cgttcagaaa ctccctccgg
      361 aactgtttcg ccaccttttt atcataaaga aatgcctgga cctcaaagtt aatttcaaga
      421 ctcctcgcgt cgaaatttgc cgacccgacg gagacggtgt gatcgtcggc gatcattatc
      481 tttgcgtgat ggaaaccttt tctgtagcta tacactttga ctcccgctct caaaagttct
      541 tctacggaag aaagagatgc ccagtgcacc atcgcgtggt cggcggcgtg aggaatgatt
      601 acccgtacat cgacgccgct catggatgcg actgtcagag cggtcgaaag ggtttttccc
      661 gggataaggt acggggtgca gagccaaaga gactctcttg cgttggnnnn nnnnnnggag
      721 aagtagccgt gcagaataga tttccagttt gaatcaggtc cgctggaaac gatctgcaca
      781 ggagtgatct cgttgtggac agcgttcggt atgtcatgcg ccggaggggc ttttaaatcg
      841 cagaaagtcc agtctgccgc gaaaatttca tcaagacatg ctaccgcttc accttcaatc
      901 ttcagatgcg tatcccgcca gattcccagt cccggctccc cgtgaatata cgaatcgcct
      961 atgttcaagc ctcctagata tccggtgatg ccgtcgatga caatgatctt cctgtggttt
     1021 cggaaattga gatcgcggcg aagcatgggg aaggagactg aaagaaaaga atgaacatgg
     1081 acaccgccgt gtttgagggt atcaatgaat tttttcccca gcttccagct gccgacactg
     1141 tcataaatca ggcgcacatc gacgccttca gcagcttttt ccaaaaggat gtctctgatt
     1201 tctccgccga ttgcgtcgtt gtcgatagag taatactcaa ggtgaatgta tcttttcgcc
     1261 tgccggagcg cgtgttttat agaggcgaag gtggcttcgc cgttcaccag catttctact
     1321 gagttatgag tcgaaacccg cgccttggcg ctttttgtca gcaaggatgc cgtttttttc
     1381 gtgtgttccc ctaagaaggc acagggttcg ttgtgttcat gagagacgaa gagggggcct
     1441 ttcctgtttt ttcttcgttt cttttttatt tttcggaaat ttcctcgttc tctgaggtcc
     1501 ggaccgaaga aaaaatagat gagaaatccg atgaccggca gcaggaggag gacgagcagc
     1561 caggcgatgg tcctgtcggg attgcgctct tcgagaaaga taatgcccgc aataaagatg
     1621 ccgtatgccc agagaaaact aaaggaatac tctttgagga aattcattac cttctctgga
     1681 atgcggtgag gcgcttggcg ccagaaatca ccgtcaagga gcagtctgat attgtctccg
     1741 atcgactccg catttgagag gatgaagaga aacgtcgcaa gtccgaaaag cagccagaag
     1801 aatgtcgatg tacgccgtat ttttttcaga gccgtctcct ccctcttttc cactcaggtt
     1861 tgaaagtgtg aatgaataaa cattcgttta cgctattcgt tttttagcag ggcttcacaa
     1921 cgccccttta tgatggtatt attccacggc atgcgtgcga gagtggtgga acggcagaca
     1981 cgctggactt aggatccagt gcctttgggc gtgggggttc aagtcccccc tctcgcacca
     2041 tcttttattt gccgaacgtg cttactgttc cctctttccg ggtaggagga gcgggaatga
     2101 atccggcgct tttgtatccc actgcaatgg agtagtacgg agcatagcct tccggtatct
     2161 ttaaagaagc cgtttcttca gaatcctcga agagaaagct cgtcaggcct atccaacagg
     2221 ttcctatgtt cagggattct gcgcccagca acatgttctg aatagctgct gcgcagtccg
     2281 cgacgacagg aaggaatgta tctcccgact gccttccgga aacgatgatg accgtaggcg
     2341 cgccatggaa cagatggtat ccttctttcg cagcaagctt ccgcacccaa tcaatcggcg
     2401 agtttgccat cttttcgatc gtggaacggc tcatctggtc aatttttgat ttgtcctgca
     2461 caacaagaaa gtgccacggt tgggtgttgt gtccgcttgg ggcccataac ccgctttcga
     2521 taattgaatt cagctcgtcg tccgtaattt ggtccggtcg aaaacttcgt acgctacggc
     2581 gtttctttat tgtctccaaa acaaggttca ttgtaatttc ctccttaaaa gaaatgtttt
     2641 tgtcgatgtg tatatgctac aatatcccaa aaatgcggaa aatgccactg tgcattttct
     2701 ttttcaccct ctgcggcagt tttttctctg cagaaaggag cggcaacact atgagaatcc
     2761 gaaataatac cgttttttgt ttcttgatat ccctgatctt ttccatcttt ttcgaggcga
     2821 gaccagctct tgcagcaccc ttctcgacga gccgagaata ccatgcgatg ctgagcgtca
     2881 ga
//
LOCUS       DAOSWW010000273         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_25931_length_2879_cov_2.946176, whole genome shotgun sequence.
ACCESSION   DAOSWW010000273 DAOSWW010000000
VERSION     DAOSWW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25931_length_2879_cov_2.946176"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..981
                     /locus_tag="PK442_06420"
     CDS             121..981
                     /locus_tag="PK442_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HOO87317.1"
                     /translation="MNTLHSARTFRGVVKIGGARGNTFLPLLEELAARVKRGEQWALV
                     HGASSMMEDLSRAVGTEPLYVTSPGGFRSRFVGEKELAIFEAACCRFSVDLVSALGRL
                     GVRAVPLYPSSAKGASAKRKDSLRSVENGKVRMLRGNYSGSIVSFDPSDVFAVWESGG
                     LPLLPPLAADEAAAGSNLNVDGDRMAAAAAAAVNADVLAILSNVPGLLRDPSDPSSKV
                     ESGTLSEWETIELFAQGNMKRKLLAAKEALEGGAETVLIADSRSAQPIEAGLSGGGTL
                     LCRGSMAAAG"
     gene            951..2075
                     /locus_tag="PK442_06425"
     CDS             951..2075
                     /locus_tag="PK442_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO87318.1"
                     /translation="MSGIYGSRGISIASGKGASVRDSEGREYLDFYCGSGAALFGHAH
                     PVLKQALVEAAELPWTIGLGMGSAVRDSFKARLNDILPDRSVFYCNSGTEAMEAALKL
                     ATSLRPGKKKVLALRRAFHGRTIGALSLTFNPQYRKEWAHLLPPVQHVKPEELPGAVD
                     ADTAVVFVEPVQGEGGVHPLAPSLGEAITEACRTTGALLVSDEIQSGWGRCGALSASP
                     LAGLEPDILCFAKGVAGGMPIGLMLWKKELGDFSASGHGSTYGGNPLSLAVANASLKL
                     LDDEGYPEKARAGGEFFRTLLAEIDSPLISEIRGMGLLNGVELTVKAVPVVKRMQEMG
                     VLALPAGPSVVRFLPPFPAGDADFLKAASVFAEALKEEGR"
     gene            2072..>2879
                     /locus_tag="PK442_06430"
     CDS             2072..>2879
                     /locus_tag="PK442_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO87319.1"
                     /translation="MNHAVELLSNLVAIPSLSGREEHACAFLADALPAAGWERVEIDG
                     AGSVVASRGSGSRELILMGHIDTVPGGPAYALDGDILRGRGSVDAKGPLCSFAVAGGR
                     APIAPDWKLTLIAATGEEADSRGALHRIPKHAPAACIIGEPSGADGVTIGYRGSLRIA
                     LSAEDGGAHRSGDAGPITAVVRAAADILSFVESADSPELPVIQRPSGAVVSMHGEENG
                     RRLGRSDLDIRLPEGSDPQQWIERIVELAGRRGVHARTLAVTPAHVADKNN"
BASE COUNT          463 a          959 c          996 g          461 t
ORIGIN      
        1 gctgcgggaa gcgcggtcca gtcggcgaac attctgctcg gcctgcccga aaccgaaggg
       61 ctcaacatgc tcccggtcta cccggcgtag cgcatcactc tatagaaagg agcttctgcc
      121 atgaacactc tgcattcggc acggacattc cggggcgtgg tcaagatagg cggcgcgcgc
      181 gggaatacat ttcttcctct tctggaggag ctggcggcgc gcgtcaagcg cggagagcag
      241 tgggcgctcg tccacggcgc aagctccatg atggaggacc tctcccgcgc tgtcggcacg
      301 gaaccgctct acgtcaccag cccgggcggc ttccgcagcc gtttcgtcgg cgagaaggag
      361 ctggccattt tcgaggcggc ctgctgccgc ttctcggtcg acctcgtgag cgcgctcggc
      421 cgtctcggcg tccgcgccgt ccctctctac ccctcgtcgg cgaagggagc gtcagccaag
      481 cggaaggact ctctccggag cgtcgagaac ggcaaggtca ggatgcttcg cgggaactac
      541 agcggctcca tcgtgtcgtt cgacccttcg gacgtcttcg ccgtctggga gtcgggcggt
      601 ttgcccctcc ttcctccgct cgcggcggac gaggccgcag cgggatcgaa cctcaacgtg
      661 gacggcgacc ggatggccgc ggccgcggct gcggcggtga atgcggacgt cctcgccatc
      721 ctcagcaacg ttccggggct gctccgcgat ccctccgacc cgtcctcgaa ggtggagagc
      781 ggcacgctct cagagtggga gacgatcgaa ctcttcgcgc aggggaatat gaagcggaag
      841 ctgctggccg cgaaggaagc cctcgaaggg ggcgcggaaa cggtgctcat cgcggacagc
      901 cgaagcgcgc aaccgataga agccggtctt tcgggaggag gtactctgct gtgtcgggga
      961 tctatggcag ccgcgggata agcatcgcgt ccgggaaggg cgcgagcgtc agggacagcg
     1021 aaggtcggga gtacctcgac ttctactgcg gcagcggggc ggcgctcttc ggccacgcgc
     1081 atcctgtact gaagcaggcg ctcgtcgagg cggcggaact gccgtggacc atcggccttg
     1141 gcatggggtc cgcagtgcgc gacagcttca aggcgcgcct gaacgacatc ctcccggacc
     1201 gttccgtctt ctactgcaac agcggcacgg aggccatgga ggccgcgctg aaactcgcga
     1261 cgtcgctccg tccggggaaa aagaaggtgc ttgcgctgcg gcgcgccttc cacgggcgga
     1321 cgatcggggc gctctcgctc acgttcaacc cgcagtaccg gaaggagtgg gcgcatcttc
     1381 tgccgcccgt ccagcacgtg aagccggaag agctccccgg cgcggtggac gccgatacgg
     1441 ccgtcgtctt cgtcgagccg gtacagggcg aagggggcgt ccatccgctt gccccgtcgc
     1501 tcggggaggc gatcacggag gcctgccgta caacgggcgc gcttcttgtc agcgacgaga
     1561 tacagagcgg ctgggggcgc tgcggcgctc tctccgcgag tccgctcgcc gggctggaac
     1621 cggatattct ctgcttcgcg aagggcgtcg cgggggggat gccgatcgga ctcatgctct
     1681 ggaagaagga actcggcgac ttctccgcct ccggacacgg ctccacgtac ggcggcaacc
     1741 cgctctccct cgccgtggcg aacgcttcgc tgaagctgct tgacgacgaa ggctaccctg
     1801 aaaaagcgcg tgccggcgga gaattcttca gaacgttgct ggcggagatc gactcgccgt
     1861 tgatttcgga aatccggggc atggggctgc tgaacggcgt ggagctcacc gtcaaggccg
     1921 ttcccgtggt gaagcggatg caggagatgg gcgtgctcgc cctccctgcc gggccgtccg
     1981 tcgtccggtt tcttcctccc ttccccgcgg gcgacgcgga cttcctgaag gccgcgtccg
     2041 tcttcgccga agcgctgaag gaggaagggc ggtgaaccat gctgtcgaac tgctgtcgaa
     2101 ccttgtcgcc atcccgagtc tgagcgggcg ggaagagcac gcgtgcgcct ttcttgccga
     2161 cgcgcttccg gcggccggat gggagcgcgt ggagatcgac ggcgcgggga gcgtcgtcgc
     2221 ctccagggga tcgggaagcc gcgaactcat cctcatgggg catatcgaca ccgtccccgg
     2281 cggcccggcc tacgcgctcg acggggatat tctccgcggc cgcgggagcg tcgacgcgaa
     2341 ggggccgctc tgctccttcg ccgtcgcagg cggacgcgcg ccgatcgctc ccgactggaa
     2401 gctcacgctc atcgccgcaa ccggcgagga ggccgactcc agaggcgcgc tccaccgcat
     2461 ccccaaacac gcccctgccg cctgcattat cggcgagccc tccggggcgg acggcgtcac
     2521 catcggctac agggggagcc tccgcatcgc gctctcggca gaggacgggg gcgcgcaccg
     2581 gagcggcgac gcgggcccca taaccgcggt cgtgcgggcg gcggcggata tactctcgtt
     2641 cgtggagtcc gccgactccc cggagttgcc ggtcattcaa cggccttcgg gcgccgtcgt
     2701 ctccatgcac ggcgaggaga acggccgccg gcttggcagg agcgacctcg acatccgcct
     2761 tccggaggga agcgatccgc agcagtggat cgaacgcatc gtcgaactcg ccggacgccg
     2821 gggcgtacat gcacgtaccc tcgccgtcac tcccgcgcac gtcgccgaca agaacaatc
//
LOCUS       DAOSWW010000274         2864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26142_length_2864_cov_5.170167, whole genome shotgun sequence.
ACCESSION   DAOSWW010000274 DAOSWW010000000
VERSION     DAOSWW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26142_length_2864_cov_5.170167"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PK442_06435"
     CDS             <1..201
                     /locus_tag="PK442_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87320.1"
                     /translation="IGEWVGSLEGLGYLMMQANARLRVDMVFACVVLLSCIGLLLWGG
                     VSRLEARYVRWNQDTKEIQTNK"
     gene            217..1155
                     /locus_tag="PK442_06440"
     CDS             217..1155
                     /locus_tag="PK442_06440"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO87321.1"
                     /translation="MRTKICCIAMFFLLLATSAAKGTEKLVLMLDWFPNVDHVPIYAA
                     IESGFFEKRGLFVEIQSPSDSADPLKLAAAGHADIALSYEPQILIAASEGIPLKALGK
                     LIGTPLTTLLFLETSGIRVPSDLEGKTIGYTVPGMMDHLAEAFASVNAIRNYKLLNVG
                     FSILQSLTSGTADAVMGPFKTYEPVLLEMEGYESRFFEIDHFGIPTYDELVFVTGDRT
                     YKEKKESIMNFLAAIDEALVFTAAHPSEALALYFRAVPEAPAEMERTAFDRTLPFFAP
                     DTTLSLDQWETFSEFARTCGMIEKKVDLNALLVSDR"
     gene            1130..1657
                     /gene="thiW"
                     /locus_tag="PK442_06445"
     CDS             1130..1657
                     /gene="thiW"
                     /locus_tag="PK442_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HOO87322.1"
                     /translation="MRFWFQTDKTGDRSRVEKLTLAGLFVAAAILLSGVHFPMGPTRC
                     YPFQHTVNVLAGAFLGPWWAAGVAFITSVIRNMTGMGTLFAFPGSIPGAIAAGLAFKF
                     FSRPVSALAEPLGTGPVGAALSALLLGPAMGKPVGFLTLQTAFLISSIPGALLGMGIL
                     HIFQKAPFSRVLRGR"
     gene            1817..2794
                     /locus_tag="PK442_06450"
     CDS             1817..2794
                     /locus_tag="PK442_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87323.1"
                     /translation="MNPVIQLNVLKCILSIRRTKTEKTNIFFYEILSPAGLFASGIAF
                     SPDDDEKSKKINVEVGAEAFLSFTEELSTRQEAFGKLLSPSVLSSLERLLGEILTAEA
                     VREALENPRDNMKTLEECLREEISSRLGYRATAYLKADRRGEEALAAFLKKDGAVLSS
                     EDANKESAPENISFLVPCAPYIDPVKGTPVASLQKGDFVLVRLPEDEAAPIREKLQRA
                     DPDFDGTVRGEVISVHKDRHGNFTLLVRLSDEFNGVTTLEGNSRIRTAPLAQPATVLP
                     GTAAGSKEKEETPDLPQPSPGDTPGWVYFFVFGAMATLVVILFFIRNFL"
BASE COUNT          644 a          762 c          772 g          686 t
ORIGIN      
        1 ataggcgaat gggtcggctc gttggagggg ctcggatatt tgatgatgca ggccaacgct
       61 cgcctgcgag tcgacatggt cttcgcgtgc gtggtacttc tttcgtgtat agggctcctg
      121 ctctgggggg gcgtttcccg ccttgaggcc cgctatgtcc ggtggaacca agacacgaaa
      181 gagattcaga cgaataagta aacgggagga atgtatatgc gaaccaagat ttgttgtata
      241 gcgatgtttt ttcttctgct ggcaacctct gctgcaaagg ggacggaaaa gctggtcctg
      301 atgctggact ggttcccgaa cgtggaccat gtgccgatct acgcggcgat tgaatcgggc
      361 ttcttcgaaa aacgcgggct cttcgtcgaa atacaaagcc cttcggacag cgccgaccct
      421 ctgaaactgg cggcggccgg tcatgccgac attgcgctct cttacgaacc gcaaatattg
      481 atcgccgcct cggagggtat tcctctgaaa gccttgggaa agctcatcgg aactcccctg
      541 acaacccttc tctttcttga aacaagcggg attcgcgttc cctctgatct tgagggaaag
      601 acgatcggct acactgttcc ggggatgatg gatcaccttg cagaggcttt cgcttcggtg
      661 aacgcaatcc gcaactataa actgctgaac gtcggtttct caatacttca gtcgctaacc
      721 tcaggaacag cagacgcggt tatgggcccg ttcaagacgt acgagcccgt tctgctggaa
      781 atggaaggat atgagtcccg tttttttgaa atagatcact tcgggatacc gacatacgac
      841 gagctcgtct tcgttaccgg tgatcggaca tacaaagaaa agaaagagag catcatgaat
      901 tttctggcag ccatcgacga ggcgctcgtc tttacggcgg ctcacccctc ggaagccctt
      961 gcactctatt tccgggctgt tccggaagct cccgccgaga tggaaagaac agcatttgac
     1021 aggacgctgc ctttttttgc gccagataca acgctttcgt tggatcagtg ggagacgttt
     1081 tctgaatttg cgcgcacctg cggaatgata gaaaagaagg tggatctgaa tgcgcttctg
     1141 gtttcagaca gataaaaccg gagacagaag ccgggtcgaa aaactgaccc tcgcaggtct
     1201 ttttgtggcg gcggcgattc ttctttcagg agttcatttc cctatggggc cgacgcgatg
     1261 ctatccgttt caacatacgg tcaacgtgct tgcgggggcg tttctcggtc catggtgggc
     1321 tgcgggagtc gcctttatca ccagcgttat ccgaaatatg acgggcatgg ggacgctgtt
     1381 cgcgtttccc ggaagcatcc cgggcgcgat cgctgcgggt ttggctttca agtttttctc
     1441 ccggcctgtc agcgcgcttg ccgaaccgct cggtaccggt cctgttggag ctgcgctctc
     1501 ggcgcttctg cttggtcccg ccatgggaaa gcctgtcggc tttcttacgt tacagactgc
     1561 atttcttatc agcagcatac ccggggcgct tctcggaatg ggtattcttc acatctttca
     1621 gaaggcgcct ttcagcaggg tactccgagg gcggtaagaa aactacggag cgattccttc
     1681 cattcttctc agggacgaac ccgctccgta gttttcgaat gcatattcca gcctatttgg
     1741 cgtccggggc tttcgcgaac agagaaaaca acgtataatg ccattctcgg aatcaatacc
     1801 gaaagggaga aaacctgtga atcctgtaat acagttgaac gttttgaagt gcatcctctc
     1861 aatacggagg acaaaaacgg aaaagacaaa catctttttc tacgaaattt tatcccctgc
     1921 gggactgttc gcctccggta tagcgttttc accggacgat gacgaaaaga gtaaaaaaat
     1981 taacgtcgaa gtgggagccg aggcatttct cagcttcacc gaagagctct ccactcgtca
     2041 agaagctttc gggaagctgc tctctccctc tgtcctctcc tctctggagc gcctcttagg
     2101 cgaaatactc accgcagagg cagtaagaga ggcgttggaa aatccgcgcg ataatatgaa
     2161 aaccctggaa gagtgcctcc gggaagagat ttcttcccgg ctcgggtatc gagcaactgc
     2221 gtatctcaag gcggatcgca ggggagagga ggcgctcgct gcgttcctta aaaaagacgg
     2281 cgccgttctt tcctccgaag atgcaaacaa agaatcggcg cccgagaata tctcatttct
     2341 cgttccttgc gctccctata ttgatccggt aaaggggact ccggtagcct cgttacaaaa
     2401 aggggatttc gtgctggtac gtctccccga agatgaagcg gctcccatcc gggagaagtt
     2461 acagcgtgcc gaccccgatt tcgacggtac agtacgcgga gaagtcatat cggtccacaa
     2521 agaccggcac ggcaacttca ctctgctggt ccgactctcg gacgaattca acggggtaac
     2581 aaccttggaa ggcaacagcc gcatacgcac cgctccgctc gcgcagcccg caaccgttct
     2641 tccgggaacg gccgcaggct cgaaagaaaa agaggaaacg ccagatcttc ctcagccttc
     2701 tcccggcgat actccgggtt gggtgtattt tttcgtgttc ggcgctatgg ctacccttgt
     2761 cgttatcctc ttttttattc gtaacttcct ctgataacag ccttcgcgag gagagacgcg
     2821 gctgcttgta tatcttcctg tccgactacc gaagcgacga ccga
//
LOCUS       DAOSWW010000275         2862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26184_length_2862_cov_3.677948, whole genome shotgun sequence.
ACCESSION   DAOSWW010000275 DAOSWW010000000
VERSION     DAOSWW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26184_length_2862_cov_3.677948"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PK442_06455"
     CDS             <1..367
                     /locus_tag="PK442_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HOO87324.1"
                     /translation="ARLLAMLRPKTAIISCGKENPYGHPHREAVALLEEAGVDLFTTI
                     GGDVVVETDGIEFTVRKVLESEKSGQEKERFYIGNRSSRVFHTSACDGLPSERNRVYF
                     KSAEEAVKAGYRPCGRCVQ"
     gene            364..579
                     /locus_tag="PK442_06460"
     CDS             364..579
                     /locus_tag="PK442_06460"
                     /inference="COORDINATES: protein motif:HMM:NF022659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HOO87325.1"
                     /translation="MRAFIDSVHEGVARVLLGDDESLAVEIPVSWLPEGAAEGKALRV
                     LWELDEEETFCARKAVEDLYDELGDMP"
     gene            complement(656..2431)
                     /locus_tag="PK442_06465"
     CDS             complement(656..2431)
                     /locus_tag="PK442_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87326.1"
                     /translation="MKKHVLRIMLAALTLLLVSSACWADMGEIQNFTFRGVGEDVLGP
                     GEDPTPDGKQDYFFSVTVSGIGALSGFILQNEDGNSVWDTVAGNNVPGMTVKDSSGNI
                     IVHSGGSMPLVPLILGAGFTISVHDDGSIARGGKFALLARFADGSRSQSVIEIPASEQ
                     PADAAASVKILSAEWTGTGTRDLTRGDEQLRGDGKADMTARISVQGKGVLTGITIRSV
                     EGEKGAWDTVPANRAPLIAVTQGNKTLNAADGNIEIPLDGKYTFDLLLTDTGAIARGK
                     SRFEVVLVFSDGSVVAKEIAKGKSEEPSSGEFSGSAVFAGPGNRDLVGRNEQRRGNGS
                     PDWKIDLKIETLGTISGIKVFNSTGPAGEWDTIPGNGRWLVAVTGADGKLLNTADGSV
                     RIAVPNPLTLQLWVEDNGSLEDPNVRSKVTLFYDDGRELTREIVSSGSKEDKTPTPTP
                     RVRPDRRRERVKREIFFSNPRQASSSDYVGSKDKIGKSGKKDWLFEFRIEGNGAVKQI
                     TVEAVDASFIWDTVPKNRNWVIGVLAPGKGLLNKKDGSVSFEVPPHNNLQLFVEDNGV
                     LKQGASQFEITVTWSDGATSKVSNF"
     gene            complement(2469..>2862)
                     /locus_tag="PK442_06470"
     CDS             complement(2469..>2862)
                     /locus_tag="PK442_06470"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOO87327.1"
                     /translation="VLLALAEARGTRSELIPNIATGFCSGMARTCGTCGALTGAIMGI
                     GLALGRSAAAESVEPAYRAVRQLIAEFEEEFGARDCHLLLGCDLGTPEGQAVFREKGL
                     RDRCAEYTGKAAEIAARVIAEIEEERRP"
BASE COUNT          519 a          881 c          844 g          618 t
ORIGIN      
        1 cgcgcggctt ctcgcaatgc tccgcccgaa gacggccatc atctcctgcg gcaaggagaa
       61 tccgtacgga cacccgcacc gggaagccgt ggcgctgttg gaagaggccg gcgtcgacct
      121 gttcacgacg atcgggggcg acgttgtcgt cgagacggat ggaatcgagt ttaccgttcg
      181 gaaagtcctt gaatcggaga agtccggtca ggagaaagag cgtttctata tagggaaccg
      241 ttccagccgg gtgttccata catccgcgtg cgacggtctc ccttccgaga gaaaccgtgt
      301 gtacttcaaa agcgcggaag aagctgtaaa agccggatac cggccgtgcg ggaggtgcgt
      361 tcagtgaggg cgttcatcga ttcggtgcat gagggggtag cgcgcgttct gctcggcgac
      421 gacgagtcgc tcgccgtgga gatacctgtc tcctggctgc ccgagggggc ggccgagggg
      481 aaggcgctcc gggtgctgtg ggagcttgac gaagaagaga cgttctgcgc gcggaaggct
      541 gtggaagatc tctacgacga gcttggcgac atgccgtgag gaaatgcgct gaacggatgg
      601 gagagctaca gaaaaaggga gaaggcgctc gtccttctcc ctttgttacc acagattaaa
      661 aattggatac ttttgaagtg gcgccgtcgc tccaggtcac cgtgatttca aactgcgacg
      721 ctccctgttt caacaccccg ttgtcctcca cgaaaagctg aaggttgttg tgcgggggaa
      781 cctcgaagga gaccgatccg tccttcttgt tcaacagccc cttccccgga gccagtacgc
      841 cgataaccca gttcctgttt ttcgggacgg tgtcccagat gaacgaagcg tccacggcct
      901 ctaccgtgat ctgcttgacg gcgccgtttc cctcgattct gaattcgaag agccagtctt
      961 tcttgccgct cttacctatc ttgtccttcg atccgacgta gtcgctgctt gaggcttggc
     1021 ggggattcga gaaaaagatc tcccgcttca ctctctcacg ccgtctgtcg gggcgcactc
     1081 tcggagtggg cgtcggcgtc ttatcctcct tcgagccgga ggagacaatt tctctggtga
     1141 gctctcttcc gtcgtcatag aacagtgtga cttttgagcg gacgttcgga tcttcgaggg
     1201 aaccgttgtc ctctacccag agttggagcg tcagggggtt cggaacggct atccggacgg
     1261 agccgtccgc ggtgttcagg agttttccgt ccgctcctgt gacggcgacg agccagcggc
     1321 cgtttccggg gatcgtgtcc cactcgcccg caggtccggt cgagttgaaa acttttatcc
     1381 cgctgatcgt cccgagcgtc tcgatcttga ggtcgatctt ccagtcgggg cttccgttgc
     1441 ccctgcgctg ctcgtttctc cctacaagat ctctgtttcc cggaccggca aaaacggcgg
     1501 aacccgagaa ctcgccggaa gatggctcct cgctctttcc ctttgcaatc tctttggcga
     1561 cgacagatcc gtccgagaag acgaggacga cttcaaagcg cgacttcccg cgggcgatcg
     1621 ctcccgtgtc cgtcagcagc aggtcgaaag tgtatttgcc gtcgagagga atttcgatgt
     1681 ttccgtccgc tgcgttcagc gtcttgttcc cctgcgttac tgcgataaga ggcgcgcggt
     1741 tggcaggtac ggtatcccag gcgcccttct ccccttcgac gctccggatg gtgatgccgg
     1801 tgagcacgcc cttgccctgg acggaaattc gggccgtcat gtcagccttt ccgtcgccgc
     1861 ggagctgttc gtctccgcgg gtaagatcgc gcgtccccgt tcccgtccat tcagcggaga
     1921 gtatcttgac cgaagccgcc gcgtccgccg gttgctcgga agcgggaatc tcgatgacgg
     1981 attggctccg cgaaccgtcg gcgaatcgcg ccagcagggc gaactttccg cctcgcgcaa
     2041 tagatccgtc gtcgtgcacg gagatcgtga atcccgcccc gaggatcaac ggaaccaacg
     2101 gcatgctgcc gccgctgtgg acgatgatat tgccgctgct gtccttcacc gtcattccgg
     2161 ggacgttgtt cccggcgacg gtatcccaca cggagttccc gtcctcgttt tgcagtatga
     2221 acccggaaag ggcgcctatc cctgaaacgg tcaccgaaaa gaaataatcc tgtttcccgt
     2281 ccggcgtagg gtcttcgccg gggccgagca catcctcgcc gacacccctg aacgtgaagt
     2341 tctgaatttc tcccatgtca gcccagcagg cggacgaaac cagcaaaagt gtcaatgccg
     2401 cgagcatgat gcgcagaaca tgttttttca cctgaaccat ctcctttgta ggtatttccc
     2461 cgttattgtt atgggcggcg ctcttcctcg atttccgcta tgacgcgcgc cgcaatctcc
     2521 gcggctttcc ccgtgtactc ggcgcagcga tcgcgaagcc ccttctcccg aaaaacagcc
     2581 tgtccttccg gcgtcccaag gtcgcacccg agcagcaggt gacagtcgcg cgcgccgaac
     2641 tcctcttcga actcggcaat gagctgcctg accgccctgt aggcaggctc caccgactcg
     2701 gccgccgcag accggcccaa cgcgagtcca atccccataa ttgcgcccgt taacgctccg
     2761 caggtcccgc aggtacgcgc catcccgctg caaaagcctg tcgcgatgtt cgggattagt
     2821 tcggatcgcg tcccccgggc ttcggccagg gcgagtagca cg
//
LOCUS       DAOSWW010000276         2860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26214_length_2860_cov_3.767914, whole genome shotgun sequence.
ACCESSION   DAOSWW010000276 DAOSWW010000000
VERSION     DAOSWW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26214_length_2860_cov_3.767914"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PK442_06475"
     CDS             complement(<1..653)
                     /locus_tag="PK442_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HOO87328.1"
                     /translation="MKKMTTHLRALIARPGALVVPGVYDALSARICEMVGFEAVFHSG
                     YGTSAVHLGQPDIGLLDMTEMSSQVRSIARAVRIPVLGDSDHGFGNAVNAYRTVQEYL
                     WAGAAGLFMEDQVSPKRCGHMEGKQVISKEEMMGKLRAALDARNELDPDFVVMYRTDA
                     IHVTWFEDALERAKAAVDVGVDMVFVEALETREQMERVVAEVKVPLMLNLIEGGKTPL
                     "
     gene            complement(650..1549)
                     /locus_tag="PK442_06480"
     CDS             complement(650..1549)
                     /locus_tag="PK442_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HOO87329.1"
                     /translation="MEKRGAQLRKLLARREILVAPGAYDGLSARLVESAGFEAVYVTG
                     YGLSASVLGKPDTGLLTLTEMASRVSAIAETVSVPVIADGDTGYGNAVNVARTVREYE
                     KAGAACIQLEDQVAPKKCGHMLGREVIPAAEMAGKIRAACDARRDDDFLVMARTDART
                     THGIDEAIERGALYEEAGADILFIESPESVEEMRRITSSFGVPVIANMVEHGRTPFLK
                     TSELEEIGYDIVIFPVGPLYAAAKAVGAVLEKLKRAGTTADCIKDMIPFAEFNALMGL
                     DGIRDMEKKYATGRDGRTEEENA"
     gene            complement(1563..2858)
                     /locus_tag="PK442_06485"
     CDS             complement(1563..2858)
                     /locus_tag="PK442_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO87330.1"
                     /translation="MIGSLLSRLYFVVVLLIGILFMGLPVAFSLGGTSLVLIWLFELP
                     MKVVGATIFTSLEGFVLLAIPLFVLMSQILLDGRIGDDLFDVMNAWVRHLPGGLAIAT
                     ILACAFFAAITGSGAATAATIGMVAYPAMTERGYDKRFTLGLLAAGGTLGILIPPSIP
                     LILYGAITEESVGKLFIAGVVPGLVLTALFIVYAVYKSKRGGFTPMERTSWSERLRVT
                     RKNIWGIFLPFLIIGGIYSGAFTPTEAAAVGLVYSLFITLFVYRTLSFRDIPAICLRS
                     VTTSCMVAIIIAGAILFGRVMTLLRIPQILTELVISNNFSVLMFIIAMNVLMMILGCI
                     LETVSIILLTMPLVTPILHALGIDPIWYGIVVTVNMTLALITPPVGMNLYVINGLRSD
                     IRMGEVIQGVTPFIVIMALFLIGVIAFPAMSTWLPSIMQ"
BASE COUNT          589 a          933 c          826 g          512 t
ORIGIN      
        1 agcggcgtct tgcccccctc gatgaggttg agcatcagcg gaaccttgac ctccgcgacg
       61 acccgctcca tctgctccct ggtctccaag gcctcgacaa agaccatgtc cacgccgaca
      121 tccaccgccg ctttcgctct ttcaagcgca tcttcgaacc acgtcacgtg gatggcgtca
      181 gtccggtaca tcacgacgaa gtcgggatcc agctcatttc tcgcgtcgag cgccgcccgc
      241 agcttgccca tcatctcctc tttggagatg acctgcttcc cctccatatg cccgcaccgc
      301 ttcggcgaca cctgatcctc catgaagagg ccggccgccc ccgcccagag gtactcctgc
      361 accgtccggt acgcgttgac cgcgttgccg aacccgtggt ccgagtcgcc caaaacggga
      421 atccgcacgg ctctggcgat cgagcgaacc tgggaggaca tctccgtcat gtcgagcagc
      481 ccgatgtccg gctgtccgag atggactgcc gacgtgccgt atcccgagtg aaagaccgcc
      541 tcgaagccga ccatctcgca gatgcgcgcg ctcagcgcgt cgtacacccc cggaacgaca
      601 agcgctccgg gacgcgcgat cagtgcgcga agatgcgttg tcatcttctt catgcgttct
      661 cctcctccgt ccggccatcg cggccagtgg cgtatttttt ctccatatcg cgaataccgt
      721 ccagccccat caatgcgttg aactccgcga agggaatcat gtccttgata caatccgccg
      781 tggtcccggc gcgcttcaac ttctccagca ccgcgccgac ggctttcgcc gcggcgtaga
      841 gcggtccgac ggggaagatg acgatgtcgt acccgatctc ctccagctcg gaagtcttca
      901 gaaaaggagt ccgtccgtgc tccaccatgt tggcgatcac cggaacgccg aagctcgacg
      961 tgatgcgccg catctcctcc accgactccg gcgattcgat gaagaggata tcggctcccg
     1021 cttcctcgta gagcgctccc ctctcgatag cctcgtcaat gccgtgcgtc gtccgcgcgt
     1081 ccgtccgcgc catgacgaga aagtcgtcgt ccctccgggc gtcgcaggcc gccctgatct
     1141 tgcccgccat ctcagccgcg gggatcactt ctcgtcccag catgtgcccg cacttcttcg
     1201 gagcaacctg atcctccagc tgaatgcagg ccgccccggc cttctcgtac tcgcggacag
     1261 tccgcgccac gttgaccgcg ttgccgtacc ccgtatcgcc gtcggcgatg accggaacgg
     1321 aaaccgtctc cgcgatcgcc gacactctcg aggccatctc cgtaagggtg agcagtccgg
     1381 tatcgggctt tcccagcacg cttgcagaga gaccgtaccc cgtcacgtag acggcctcga
     1441 acccggcgga ctcgacgagc cgggcggaaa gaccgtcgta agctcccggc gcgacgagaa
     1501 tctcccgccg cgccaggagc ttgcgtagct gcgctcctct tttttccatg atcatccgtc
     1561 cgttactgca tgatcgacgg cagccacgtg ctcatcgccg ggaaggcgat gactccgatc
     1621 aggaagagcg ccatgatgac gatgaacggg gtgacgccct ggatgacctc tcccatccgt
     1681 atatccgacc tcagtccgtt gatgacatag aggttcatac ccactggagg cgtgatcagc
     1741 gccaatgtca tgttcaccgt gaccacgata ccgtaccaga tcgggtcgat accgagcgcg
     1801 tggagtatcg gggtcaccaa tggcatggta agcaggatga tggagaccgt ctccagtatg
     1861 cagccgagga tcatcatcag cacgttcatc gcgatgatga acatcagcac ggagaagttg
     1921 ttgctgatga cgagttcggt gaggatctgc ggaatccgca gcagcgtcat cacgcgtccg
     1981 aagagaatgg cgccggcgat gatgatcgcc accatgcacg acgtagtaac ggagcggagg
     2041 cagatcgccg ggatgtcgcg gaaactcagc gtcctgtaga cgaagagcgt gatgaaaagg
     2101 ctgtagacaa gccccacggc tgcggcctcg gttggagtga atgcgccgga ataaatacct
     2161 ccgataatga ggaaggggag gaagataccc cagatgttct tccgtgtaac cctgaggcgc
     2221 tcgctccagg aggttcgctc catgggggta aaaccgcccc gcttgctctt gtagactgcg
     2281 tacacgataa agagcgccgt caggacgaga ccggggacaa cccccgcgat gaagagcttt
     2341 ccgacggact cctcggtaat cgcgccgtag aggataaggg gaatgctcgg cggaatgagg
     2401 atgccgagcg tgccgcccgc ggccagcaga ccgagagtga atcgcttgtc gtatccgcgc
     2461 tccgtcatcg ccgggtaggc caccatcccg atcgtcgccg cggttgccgc gccggagccg
     2521 gtgatggcgg cgaagaaagc gcacgcaaga atcgtcgcga tggcaagccc gccgggaagg
     2581 tgccgcaccc acgcattcat gacatcgaaa aggtcgtcgc cgatccgtcc gtcgagcagt
     2641 atctggctca tcagcacgaa gagcggaatg gcgagcagca caaaaccttc gagcgatgtg
     2701 aatatggtcg cgcccacgac tttcatggga agctcgaaaa gccagatcag cacaagcgac
     2761 gttccaccga gagaaaaggc caccggaagt cccatgaaga gaattccgat gagcagcacg
     2821 acgacgaagt acaaccggct gagcaacgag ccgatcacga
//
LOCUS       DAOSWW010000277         2859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26234_length_2859_cov_2.794223, whole genome shotgun sequence.
ACCESSION   DAOSWW010000277 DAOSWW010000000
VERSION     DAOSWW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26234_length_2859_cov_2.794223"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PK442_06490"
     CDS             complement(<1..426)
                     /locus_tag="PK442_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOO87331.1"
                     /translation="MQTLFRGKHFITLQEWTKEEVDTLLDVSYDLKKDFAMDRPTNYL
                     PNKTVFLMFFEQSTRTRNSMEAGITQLGGHAHFLDTSNMQIAHGEVPKDTAVILSRMG
                     HAIACRNCFWKEGNAYLREMATWSPVPIISMQDDLYHPLQ"
     gene            complement(475..1683)
                     /locus_tag="PK442_06495"
     CDS             complement(475..1683)
                     /locus_tag="PK442_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOO87332.1"
                     /translation="MIPFEKVLSKAEEYKPKMSRFLRDMIALPSESCGEKEVIHRIKA
                     EMEACGYDRVEIDPMGNVLGFIGNGKHLIALDAHIDTVGIGDRTLWTYDPYEGYEDDE
                     IIVGRGASDQEGGMASMVYAGKIIKDLGLPGDFTLMVVGSVQEEDCDGLCWQYIINED
                     KYTPEFVVLTEPTSCNIYRGHRGRMEIKVSTRGLSCHGSAPERGDNAIYKMAPILQEL
                     RALHENLHFDPFLGKGSLTVSEVFFSSPSRCAVADGCTISVDRRLTHGETWEMALQQI
                     RNLPAVKAAGAEVSMYSYDRPSWTGLVYPTECFFPTWVLEENHAACRSMVDGYRDLFK
                     SEPLVDKWTFSTNGVSIMGRYGIPCVGFGPGHEDQAHAPDERTWKSELVKAAALYSVV
                     PSIYTGKYVG"
     gene            complement(1718..>2859)
                     /locus_tag="PK442_06500"
     CDS             complement(1718..>2859)
                     /locus_tag="PK442_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO87333.1"
                     /translation="VDMFTLMAMPFFILAGEIMNKSGITGRLVKFANVLVGHLQGGLA
                     HANILASVFFAGITGAAVSDTAAIGSMLIPAMVEEGYDKDFSAAVTASSSIIGPTIPP
                     SNIMVIYGAFMQVSIAGLFLAGVIPGLLVAAALMVLTARISKKRGYPVGERRATFKEM
                     AVAFKEAFVALLMPAIILGGILSGIFTPTEAASVAVAYAMFIGFFVYRNLTLKDMWPI
                     FMKMARTTGVVFLVIAAASILGWVLTIEQIPEKVATLMLSVSTNKWVVMGMILLLLLF
                     IGMFMDIAAALIILGPILHPLAVKIGFHPLHFGIIMVLALNIALMTPPVGACLFVACG
                     ISKLTIEQLSKEIFPFILVVVAVLLLITFVPEIPLFLPRLMGMVH"
BASE COUNT          584 a          852 c          852 g          571 t
ORIGIN      
        1 ctgcaggggg tggtacaggt cgtcctgcat gctgatgatg ggcacgggag accacgtggc
       61 catctcccgg agataggcgt tcccttcctt ccagaagcag ttcctgcacg caatggcgtg
      121 acccatgcgg gacaggatga cggccgtgtc cttgggcacc tcgccgtggg cgatctgcat
      181 gttgctcgtg tcgaggaagt gggcgtgccc gccgagctgg gtgatgccgg cctccatcga
      241 gttgcgggtg cgggtggact gctcgaagaa catcaggaag accgttttgt tgggaaggta
      301 gttcgtcggt ctgtccatgg cgaagtcctt cttcagatca taggagacgt cgagaagcgt
      361 gtcgacctcc tctttcgtcc actcctgaag cgtgatgaaa tgcttgcccc tgaaaagcgt
      421 ctgcattgtt ttttttcctc cttgagttgt actctaaacg agtcgttcgg ctctctagcc
      481 gacgtacttt cctgtgtaaa tcgacggcac gacggagtag agcgcggcgg ccttcaccag
      541 ctcgctcttc catgtgcgct cgtcgggggc gtgcgcctga tcctcgtgcc ccggtccgaa
      601 accgacgcag gggatgccgt agcgccccat gatggagacg ccgttcgtgg agaaagtcca
      661 cttgtcgacg agcggttcgc tcttgaaaag atccctgtac ccgtccacca tggacctgca
      721 cgccgcgtga ttctcctcga gcacccacgt cgggaagaag cattccgtgg ggtacaccag
      781 tcccgtccag gaaggacggt cgtaggagta catggaaacc tccgcaccgg cggccttcac
      841 cgccggaaga ttgcgtatct gctgcagcgc catctcccac gtctcgccgt gcgtgagtct
      901 gcggtccacg gagatggtac agccgtccgc caccgcgcag cgcgacggag agctgaagaa
      961 gacctcagac acggtaaggc ttcccttgcc gagaaacggg tcgaagtgca ggttctcgtg
     1021 aagggcgcga agctcctgaa gaatcggcgc catcttgtag atcgcgttgt cgccccgttc
     1081 cggagcggac ccgtggcagg agagccccct cgtgctcacc ttgatctcca tacggccgcg
     1141 gtggccgcgg tagatgttgc acgacgtggg ctcggtaagc accacaaatt ccggcgtgta
     1201 cttgtcctcg ttgatgatgt actgccagca gaggccgtcg caatcctcct cctggacgga
     1261 gcccaccacc atcagcgtga agtcgcccgg aagccccaga tccttgatga tcttccccgc
     1321 gtagaccatg gaggccatgc cgccctcctg gtcgctggcg cctctaccga cgatgatctc
     1381 gtcgtcctcg tacccctcgt aggggtcgta ggtccaaagg gtgcggtcgc cgattccgac
     1441 ggtatcgatg tgcgcgtcga gggcgatcaa gtgtttgccg ttcccgatga agccgagcac
     1501 gttccccatg gggtcgatct cgactctgtc gtacccgcag gcctccatct cggccttgat
     1561 gcgatggatc acttccttct ccccgcagct ctcgctggga agagcgatca tgtcccgaag
     1621 gaagcgggac atcttcggct tgtactcttc cgccttgctc aaaaccttct cgaagggaat
     1681 catggaatac cttctttcat gcgatattct taataactca gtgaaccatt cccatcagcc
     1741 tcggcaggaa gagcgggatc tccggtacga aggtgatcag aagcagcacc gccaccacga
     1801 cgaggataaa gggaaagatc tccttgctta actgttcgat cgtcagttta ctaatgccgc
     1861 aggcgacgaa gagacacgcg cctacgggag gcgtcatcag cgcgatgttg agcgcgagca
     1921 ccatgatgat gccgaagtgc agcggatgga aaccgatctt caccgcgaga gggtgcagga
     1981 tcgggccgag gatgatgagc gccgccgcga tgtccatgaa catgccgatg aagaggagga
     2041 gtagcaggat cattcccatg accacccatt tgttcgtgct cacgctgagc atgagggtag
     2101 ccaccttctc cggaatctgc tcgatggtga gcacccagcc gagaatcgag gcggcggcga
     2161 tgacaagaaa gaccacgccg gtggtgcgcg ccatcttcat gaagatcggc cacatgtcct
     2221 tcagcgtcag gttccggtag acgaagaacc cgatgaacat cgcataggcg acagcgacgg
     2281 aagccgcctc ggtcggcgtg aagatgcctg agagaatacc ccccaggatg atggcaggca
     2341 tcagcagcgc cacgaacgct tctttgaaag ctaccgccat ctctttgaat gtcgcccggc
     2401 gctctcccac aggatacccg cgcttttttg aaatcctcgc cgtgagaacc ataagggccg
     2461 cggcgaccaa aaggccggga atgacgcccg cgaggaagag tccggcaatg gacacctgca
     2521 tgaaggcgcc gtagataacc atgatgttcg acggaggtat cgtcgggccg atgatggacg
     2581 atgacgcagt gactgccgcg gagaagtcct tatcgtaccc ttcctctacc atcgccggga
     2641 tgagcatcga gccgatggcg gcggtatcgc tcaccgccgc ccctgtgatc ccggcgaaaa
     2701 agacggacgc caggatgttg gcgtgcgcca gtcctccttg aagatggccg acaagcacat
     2761 ttgcgaactt gacaaggcgg cccgtgatac cgctcttgtt catgatctct cctgccagaa
     2821 tgaaaaacgg catcgccata agggtgaaca tgtccaccc
//
LOCUS       DAOSWW010000278         2857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26260_length_2857_cov_3.047109, whole genome shotgun sequence.
ACCESSION   DAOSWW010000278 DAOSWW010000000
VERSION     DAOSWW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26260_length_2857_cov_3.047109"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2167)
                     /locus_tag="PK442_06505"
     CDS             complement(<1..2167)
                     /locus_tag="PK442_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO87334.1"
                     /translation="MLTANLIGPPRFFLDDRELFFPIRKAQALLCYLLVEERASREAL
                     SSLLWGGSEDMLASQSLRNALYLLRKGLPEGLVGTGRQWITVDMSRVDLDLLLLERLD
                     DAPLERLPALCRPLLEGFSLPNAPEFDDWLRTARYQWAGRIRSAFLALSRRCMEAEEW
                     EAALCPLNLVAAGDEIDEESYRLIMRAHAARREWGRVAEAWRVLEGRLKNELGVEPSS
                     ESLALAASLLKKRDTRTQVFEKTQPAPRKDFFFGRSSELRSVREFCGAVSASPRCIFV
                     EGEAGVGKSLFVKTALPLSFPAEPLILRCTAGNPEAAYPLLPWDELLSSLFRSVDRES
                     LALPQTLWTILGQSFPSIGSRKTSVQPRNPRRLGAMLGDLFASAGATRPLALVFEDFQ
                     WFDDPSMDVLEHLLRSSPGNLTLFFTSRAASSFRGVSLVRDAARNGRIALLEIRLSCF
                     SRRESDDFCAALLPSRPFSTEELELVFRQTEGLPLFLSELLRAVEKGLPLYEAPRHLG
                     DAIEGHLCHIDEEQRRLLEAISIFPQGAEWTVVRRLLGLSGARLAALSESLRGRGLLD
                     ENRGEGESLLLEFRHGKVREHVYAFMSEGRRRTLHESAARLLMDEFSVRPWEDLACSR
                     IIHHCKRGGLRLEELEYMIRRLKYHIKLNYELFPLLDDHILKACATSLDNRSSTADDL
                     EAARELLRALRKEKEGATPRFAALERAYFALRGGYYLWWG"
     gene            2393..>2857
                     /locus_tag="PK442_06510"
     CDS             2393..>2857
                     /locus_tag="PK442_06510"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO87335.1"
                     /translation="MIKSEHDSKQDGAFKVTAIMEALCASSSSLTVREIEERTGIPRS
                     TAHRFLVSLEERRWVFSDAATEGYRPGIRFFHLSSKSSFYDELVKKAEPGMFRLMTST
                     GNTAVLSVAEGALGSCIHSVEPLSPVKFTAHRGMTIPLHAGATGKILLAFCSQ"
BASE COUNT          671 a          865 c          790 g          531 t
ORIGIN      
        1 cgccccacca gaggtagtac ccgcctcgaa gcgcgaagta cgcccgctca agagcggcaa
       61 agcgcggcgt ggcgccctcc ttttccttcc ggagcgcgcg gagcagctcc cgtgcggctt
      121 ccagatcatc cgccgtgctc gagcgattgt ccagagaggt cgcacaggct ttcagaatat
      181 ggtcgtcgag aaggggaaag agttcgtagt tcaacttgat atgatacttg agcctccgga
      241 tcatatattc gagttcctcc aggcgcagcc ctcctcgttt gcagtgatgg atgatccttg
      301 agcaggcgag gtcctcccac gggcgaacgg aaaattcgtc catgagaagg cgggctgcgc
      361 tctcgtgcaa cgttcgcctc cgtccttcgg acatgaaagc gtacacatgc tctcttactt
      421 tgccgtgacg aaactccaaa aggagcgatt ctccctcgcc gcggttttca tcgagcaatc
      481 ctctccctct caaactctcg gacagcgcag ccaatcgtgc gccggacagc ccgagaagtc
      541 ttcgcaccac tgtccactcg gcgccctgcg gaaagatgga gatcgcctcc agaagcctgc
      601 gctgttcttc gtctatatgg cacagatgcc cctctatggc atctccgaga tgccggggcg
      661 cttcgtaaag cggcaggcct ttctcgacag ccctcagaag ttcggagagg aagagcggca
      721 gcccctcggt ctggcggaag accaattcca gttcctctgt agaaaatggg cgcgaaggaa
      781 gcaacgctgc gcagaagtca tcggactctc ttcgggagaa gcacgagagc cggatttcga
      841 ggagggcgat acgcccgttt cgggcggcat cccgcacaag ggaaacgccg cggaaggagg
      901 acgccgccct cgaggtgaag aacaacgtga gattccccgg tgacgaacgg agcagatgtt
      961 caagcacatc catcgaagga tcgtcaaacc attggaagtc ctcgaacacc agcgccagag
     1021 gacgcgttgc gccggccgaa gcgaagagat ccccaagcat ggctccgaga cgacgggggt
     1081 ttctgggctg gaccgacgtc ttgcggcttc cgatagaagg gaaactctgc cccagaatag
     1141 tccagagcgt ttggggaaga gcaagactct ctctgtcgac tgaacggaag agagacgaca
     1201 ggagttcgtc ccatggaagc aagggatacg cagcctccgg atttccagcg gtacatcgca
     1261 gtattaacgg ctcggccggg aaagaaagag ggagcgccgt cttgacgaag agactcttgc
     1321 cgacaccggc ctctccttcg acgaaaatgc atcgcggcga cgcgctcacc gcaccgcaaa
     1381 attcccgaac agagcgcaac tctgaggaac gtccaaaaaa gaagtccttc cttggagcag
     1441 gctgcgtttt ttcaaatacc tgcgttcgtg tatcccgctt tttaagaaga ctcgcggcga
     1501 gagccagact ctcgctcgac ggctccacgc ccaactcgtt cttcaggcgg ccttcaagta
     1561 ccctccacgc ctccgccact ctgccccact cgcgccgcgc cgcatgagcg cgcattatca
     1621 ggcggtacga ctcttcgtca atttcgtccc ctgccgctac aaggttcagc ggacacagcg
     1681 cagcctccca ctcctccgcc tccatacacc gccgagacaa tgcaagaaaa gcggagcgaa
     1741 ttcttccggc ccactggtag cgtgcggtcc tgagccagtc atcgaactcc ggcgcgttcg
     1801 gcagcgaaaa tccttcaagg agcggacggc ataaagcggg aagacgctcg agaggggcgt
     1861 cgtcaaggcg ttccaaaaga agcagatcaa ggtccacacg ggacatatct acggtaatcc
     1921 actgccggcc cgttcccacg agcccctcgg ggaggccttt ccgaagaagg tagagcgcat
     1981 tcctcaggct ctgggaagca agcatgtctt cgctcccgcc ccagagcaac gacgagagcg
     2041 cctcacgaga agctctttcc tcgaccagga gatagcaaag gagcgcctgg gccttccgga
     2101 tcggaaagaa cagctctctg tcgtcaagaa aaaaccgcgg agggcctatc aggttggcgg
     2161 tgagcatgaa cggctcccct ttccctcggg aaatacgaaa ttgttactga aaaactatga
     2221 acgattatac catcgacctc ctcccggaac ccaggaacac cggaaaaaca agaactcaaa
     2281 agatgaatgg agcctctcct cacacgcccc tgtcgggacc ctgccgtagc gattgacaaa
     2341 ccggcacgct catgataata atgggacaca ttgtcccatt ttggaggtcg cgatgataaa
     2401 aagcgagcac gattctaagc aggacggggc attcaaagtg acggcaatca tggaggcgct
     2461 ctgcgcatcc tcttcgtcgc tcactgtccg cgagatcgag gaacggacag gaatacccag
     2521 aagcacagcc cacaggtttc tcgtctctct tgaagaacgc cgttgggtct tcagcgacgc
     2581 tgcaaccgag gggtaccgac cgggcatccg tttctttcat ctcagcagca aatcctcttt
     2641 ctacgacgaa ctcgttaaaa aagcagagcc agggatgttc cgtcttatga caagcacggg
     2701 caatactgcg gtcctgagcg ttgcggaagg ggcgttgggg tcgtgcatcc actcggtcga
     2761 gcctctctcc cctgtaaagt tcaccgcgca cagaggcatg acgattcctc ttcacgcagg
     2821 agccactggg aaaatattgc tcgccttctg ttctcaa
//
LOCUS       DAOSWW010000279         2856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26278_length_2856_cov_2.985005, whole genome shotgun sequence.
ACCESSION   DAOSWW010000279 DAOSWW010000000
VERSION     DAOSWW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26278_length_2856_cov_2.985005"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1283)
                     /locus_tag="PK442_06515"
     CDS             complement(99..1283)
                     /locus_tag="PK442_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOO87336.1"
                     /translation="MRSEEVYAKSLELHKAKKGKVAIASKMPVNSMADLALAYTPGVA
                     EPCREIGRNKDALYEVTSKGNMVAVITDGTAVLGLGDIGPDAALPVMEGKSILFKRFA
                     DIDAFPICINSKDVDEIVRTSALIASSFGGVNLEDIAAPRCFEVEKKLQEICDIPIFH
                     DDQHGTAVIVLSGILNALKVVGKKLDAVKIVMTGAGAAGTAICKFLMSAGAKNVILCD
                     RSGAIYEGRTEHMNWAKEEIAKASNPQKEKGSVADVIKNADVYLGLSGPGIINGDMVK
                     TMAPKAIIFAMANPTPEIYPDEALAAGAAIVATGRSDFPNQINNCLGFPGIFRGALDV
                     RASVINEEMKLAAAHALASLVPDDELRADRIITEVMDDRVVPAIAKAVAEAARKTGVA
                     RI"
     gene            1701..>2856
                     /locus_tag="PK442_06520"
     CDS             1701..>2856
                     /locus_tag="PK442_06520"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HOO87337.1"
                     /translation="MWRRSGKIDSRTILVGDGGKKLVLFLFGILFCVVGFLFEPLQRI
                     LQGMACILIEPGNLFSDYFCIGGRGASFFNSGVLTILFTSILVYYEIPIRGISIASIF
                     TVAGFAFFGKNLVNVWFILAGVQIYAWSQKESFLKYVYIAFFGTALSPLVSQLMFALD
                     LPFLPRIALGASAGISAGFFLPSLTATFLSLHRGYNLYNIGFSAGMLGTLFVSLLRSH
                     GLVFEMRFVWSTEPCVLLLPFFAGLFSLMVFAGYFLNGMSFKGVGELWKHSGRLLADF
                     VDIFDFPPVLVNMGANGLIAISYLWLVGGDFNGPTIGGVLTIVGFGAMGKTPRNIIPI
                     LIGVTLGGATRGSSLNDPSLQMAALFGTTLAPIAGEFGWKAGLFAGYVHSS"
BASE COUNT          539 a          716 c          793 g          808 t
ORIGIN      
        1 atctctttac tttttctcaa ccaaccctat tgacttttga tagctggtct cctctatgat
       61 gtgagtatgc cattacagcc tctttgggag ccgcccggtc agatacgcgc cacccctgtc
      121 ttgcgcgcgg cttcggcgac cgctttcgcg atggcgggaa cgacgcggtc gtccatgacc
      181 tcggtgatga tacggtcggc tcgcagctca tcgtcgggga cgagcgatgc aagcgcgtgg
      241 gccgcggcga gcttcatctc ttcgttgatc accgaagcgc ggacatcgag cgcgccgcgg
      301 aagataccgg ggaagcccag acagttgttg atctgattgg ggaagtcgct gcgcccggtg
      361 gcgacgatgg cggctccggc ggcgagcgcc tcgtcggggt agatttcggg cgtcgggttg
      421 gccattgcaa agataatagc cttcggcgcc atcgtcttca ccatgtcgcc gttgatgatt
      481 ccggggccgg agagaccgag atagacgtcg gcgttcttga tgacgtcggc aacggagccc
      541 ttttccttct gaggattcga cgcttttgca atctcctcct tggcccagtt catgtgttcg
      601 gtccgccctt cgtagattgc gccgctacgg tcgcaaagaa tcacattctt cgccccggcg
      661 gacataagga atttgcagat ggccgtcccc gcggcgcctg cgccggtcat gacgatcttt
      721 acggcgtcca gtttcttccc gacaaccttc agcgcgttga ggattcccga gaggacgatg
      781 acggcggtac cgtgctggtc gtcgtggaag atggggatat cgcagatctc ctgaagtttt
      841 ttctccacct cgaagcagcg gggagccgcg atatcttcga ggttgacgcc gccgaaggag
      901 gaggcgatga gggcggatgt tcgcacaatc tcgtccacgt ctttcgagtt gatgcagatg
      961 gggaacgcgt cgatatcggc gaagcgcttg aagaggatgg atttcccttc catgaccgga
     1021 agagccgcgt cgggaccgat gtcgcccagg cccaataccg ccgtgccgtc ggtgatgacg
     1081 gcgaccatgt tccctttgga cgtgacctcg tagagcgcgt ccttgttgcg gccgatctcg
     1141 cggcagggct ctgcgactcc gggcgtgtac gccagggcca gatcggccat ggagttcacc
     1201 ggcatcttgc ttgcgatggc gaccttgcct tttttcgcct tgtgcagttc gagtgatttt
     1261 gcgtagactt cctcgcttct catgaaacag cctcctttaa gagatgaaaa taccgatatt
     1321 tggcttagcg catgcgcttt ttcatgcgat cgaaaaatat tgtatcacgt ccttctctgt
     1381 acaggagaag gttcgtgtat ctttttttct tgcaagtcga gatgtaaatt atagattact
     1441 tataaaaagt gtagaaaata tctccttctt tgaggtgagc tgttcattac ttgaacgcat
     1501 ggaaccgtgg tgaaaatgtt attttacata tggtgcgcat cggcattatc ataaaaataa
     1561 ttagatagta tttctcgacg cattcaatgc aaagcgagga gggcgaccaa tgggagtaat
     1621 cgtgacgcag tatatcggag ctttccaggt catgttgcga atctgccatt ggaacgtttc
     1681 acaataagtg gtgcgttatt atgtggcgcc gttcaggaaa gattgattct cgaaccatcc
     1741 tcgtcggcga cggcggaaaa aagctcgtcc tttttctttt cggtattctt ttttgtgttg
     1801 ttggatttct cttcgagcct cttcaacgca ttctccaagg gatggcgtgc attctgattg
     1861 aaccaggcaa tctgttttcc gattacttct gcataggcgg acgaggggca tcttttttta
     1921 acagcggtgt cctcacgatt ctctttacgt cgattctcgt atactatgaa atccctattc
     1981 gaggcatctc gatcgcttcg atatttaccg tagcgggatt tgcttttttc gggaaaaacc
     2041 ttgtaaatgt atggtttatt cttgcaggcg tccagatata tgcatggagc caaaaagaat
     2101 cgtttttgaa gtatgtgtac atcgcttttt tcggaacggc actctcgccg ctggtgagtc
     2161 aactgatgtt tgcccttgat cttccgttcc ttccgcgaat tgctttaggg gcgagcgcgg
     2221 ggatctccgc cggttttttc cttccttccc tcacggctac ttttctttct ctgcacaggg
     2281 ggtacaacct ttataatatt ggtttttccg caggaatgct cggaacgctt tttgtctccc
     2341 ttctgcggtc tcacggactt gttttcgaga tgcgtttcgt ctggtccacg gagccgtgcg
     2401 tcctcttgtt gcctttcttt gcggggcttt tctctcttat ggttttcgcc ggatattttc
     2461 tcaacgggat gagttttaaa ggtgtagggg agctgtggaa gcattcgggg cgtctccttg
     2521 cggatttcgt cgatatcttc gatttcccgc ccgttctcgt caatatggga gcgaacggtt
     2581 tgattgcaat ttcatatctt tggttggtcg gaggcgattt caacggcccg acgataggag
     2641 gcgtgcttac catcgtcggg ttcggtgcga tgggaaaaac tccccgaaac atcattccta
     2701 ttcttatcgg ggtgaccttg ggaggggcga caagaggttc gagtctaaac gacccttctc
     2761 ttcagatggc tgcgcttttc ggtacgacgc ttgcgcccat agcgggtgaa ttcggctgga
     2821 aggcggggct cttcgcggga tatgtgcatt cctcgg
//
LOCUS       DAOSWW010000280         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26289_length_2855_cov_3.364286, whole genome shotgun sequence.
ACCESSION   DAOSWW010000280 DAOSWW010000000
VERSION     DAOSWW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26289_length_2855_cov_3.364286"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK442_06525"
     CDS             complement(<1..311)
                     /locus_tag="PK442_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87338.1"
                     /translation="MQPLRLSCTLLLLLSLLFALPSAASAVDLIIEMDAVVSVSGNPF
                     PLSEAARLSGDPALVQKTGALLFDIPPDGLLTRDTLLNTLVAKGIGGIRLKLVMPPEM
                     R"
     gene            complement(355..1143)
                     /gene="flgG"
                     /locus_tag="PK442_06530"
     CDS             complement(355..1143)
                     /gene="flgG"
                     /locus_tag="PK442_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOO87339.1"
                     /translation="MLRALWTSASGMIAQQTNLDVTTNNLANVNTSGFKKKRADFADL
                     LYQINREPGAPVEPASMVPTGVQVGLGVRVVGTPRIMTQGNLQVTDRPLDITIEGDGF
                     FQVLQPNGEVAYTRAGGWNLDGEGQVVNTDGLLIEPAILVPEDATEIMISPEGLVSVK
                     IDGQPEPEEIGQIELARFVNSGGLRAIGKNLFLETAASGAPIVGNPGDEGLGQVHQGI
                     LEMSNVQVVDEMVNLIIAQRAYEANSKGVQTADELLRIANGLKR"
     gene            complement(1160..1969)
                     /locus_tag="PK442_06535"
     CDS             complement(1160..1969)
                     /locus_tag="PK442_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOO87340.1"
                     /translation="MHRGLYAAASAMIVQESMHDVVANNLANVNTSGFRRRIPVNKSF
                     PEVLMDRIEKKSEDGEIKLIAPPFQLGLKGKFLIGDLSLANVISETYMTTESGAIQVT
                     DNPFDAAIVGEGYFAVQDGAGNIYYTRSGHFQKNGEGQLVTEDGMLVLGDGGPIEVGD
                     ASRFAITENGNVFADGAVVDMFQVVTFENPTYLRQVGRTALAVTPQSGAPVPVEAIRL
                     EPGVLEMSNVNVVEEMVRMVEAHRAYESASKVLMTHDELTGKLISSYGRTS"
     gene            complement(2024..>2855)
                     /locus_tag="PK442_06540"
     CDS             complement(2024..>2855)
                     /locus_tag="PK442_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOO87341.1"
                     /translation="YTMTEAMMHHFMRRVLKGFNRFFRNRVMICVPSGATDVERRAVL
                     EAAVEVGAKEAYLIEEPMAAAIGANLNVEEPRGKMVVDIGGGTTDIAVISLGGIVVSK
                     SLRIGGDRFDEGIIRFLRKQYNLAIGEQTAENLKIMIGTCLQEAPDTKMILKGRDLVQ
                     GLPRQIEVSSASVSMAIGEMVQALIDGVRNVLELTPPELSADIIDRGIVLTGGGALLR
                     GLTELITRQTGINCFTADDPMECVALGTGKALAEIDKLQASGRSGILVNRRKGRKKKL
                     "
BASE COUNT          531 a          838 c          862 g          624 t
ORIGIN      
        1 atacgcatct cgggaggcat gacgagcttg agtctgatgc cgccgatgcc ctttgcaaca
       61 agggtgttga gtaaggtatc gcgcgtcaga aggccgtcgg gagggatatc gaaaaggagc
      121 gctccagtct tctgcaccag ggcaggatca ccggagaggc gggccgcctc cgaaagagga
      181 aaggggttgc ccgaaacgga gacaacggcg tccatctcta tgatgagatc gacggcggac
      241 gcggccgaag gaagagcgaa aagaagcgag aggagaagaa ggagggtgca ggaaaggcgg
      301 aggggctgca caccgctccg ccttaggaga ttgtgaatga aagacgccac tagcctacct
      361 cttgaggccg ttggctattc ggagcagttc gtccgctgtc tgcactccct tggagtttgc
      421 ttcgtacgct ctctgtgcga tgatcaggtt gaccatctcg tcgaccacct ggacgttgga
      481 catttcgagg atgccctgat gcacctgtcc gagcccttca tcgcccgggt tgccgacgat
      541 gggcgcgccg ctggccgctg tttcgaggaa gaggttcttt ccgatggcgc gaagtcctcc
      601 cgaattgaca aaccgggcga gctctatctg gccgatctcc tccggctccg gctgtccgtc
      661 tattttgacc gagaccagcc cttccgggct gatcatgatc tcggtagcgt cctcgggaac
      721 aaggatcgcg ggctcgatga gcagtccgtc cgtgttgacg acctgtcctt ccccgtcgag
      781 attccagccg cctgcgcggg tataggccac ttcgccgtta ggctgaagta cctgaaagaa
      841 accgtccccc tctatggtga tgtcgagagg acggtccgtc acctggaggt tcccctgcgt
      901 catgatgcgg ggcgttccga cgacccggac gccgagtcca acctggacgc cggtcggcac
      961 catcgacgcg ggttcgacgg gtgcgccggg ctccctgttg atctggtaga gcagatccgc
     1021 gaagtcggcc cgcttcttct tgaagccgga agtattcacg ttcgcaaggt tattggttgt
     1081 gacgtcgaga ttggtctgct gcgcgatcat cccgcttgcg cttgtccata aggctctgag
     1141 catttctttc tacctccggt taactcgtcc tgccgtagga cgagatcagt tttccagtga
     1201 gttcgtcgtg cgtcatgagc actttggagg ccgactcgta cgcgcggtgc gcctcgacca
     1261 tccggaccat ctcctcgacc acgttcacgt tggacatctc caacaccccc ggttcaaggc
     1321 gaattgcttc gaccggaacg ggagcgccgg actggggcgt gactgcaagc gcggttcgcc
     1381 ctacctgcct gagataggtg gggttttcga aggtcacaac ctggaacatg tcgaccacgg
     1441 cgccgtcggc aaaaacattg ccgttttcag taatggcaaa acgggacgcg tcgccaactt
     1501 cgatcggtcc cccgtctccg aggacaagca tcccgtcttc cgtgacgagc tgcccttccc
     1561 cgttcttttg aaagtgaccg gatcgggtgt agtagatgtt tccggcgccg tcctgaacag
     1621 cgaagtaccc ttcgccgacg atcgccgcgt cgaaggggtt gtcggtgact tggattgcgc
     1681 ctgactcggt ggtcatgtag gtttccgaga taacgttggc gagactgagg tcgccgatga
     1741 gaaactttcc tttgagcccg agctggaaag gcggggcgat gagtttgatc tcgccgtcct
     1801 cggatttctt ctcgattcgg tccataagaa cctcgggaaa cgacttgttg acgggaatcc
     1861 gtctccggaa ccccgaagtg ttgacattcg cgaggttatt ggccacgaca tcgtgcatac
     1921 tctcctgaac gatcatcgcg gatgcggcgg catagagtcc ccgatgcata ttctttcctc
     1981 cctcgatgct ccgccctggc ggaagttgct tccgcgcggc ggatcagagc ttcttcttcc
     2041 ggcctttcct gcggttcacc agtatgccgc tccgccccga ggcctgaagc ttgtcgatct
     2101 cggcgagggc ctttcccgtg ccgagcgcga cgcattccat cgggtcgtcg gcggtgaagc
     2161 agttgatgcc ggtctggcgg gtgatcagtt cggtgaggcc ccgcaaaaga gcgcctcctc
     2221 cggtaagtac aatccccctg tctattatat cggctgagag ttccggcggc gttagctcca
     2281 aaacatttcg aactccatca atcagcgcct gtaccatttc tccgatggcc atcgagacgc
     2341 tcgcgctgga gacttctatc tggcgaggaa gcccctggac caggtctcgc cccttgagaa
     2401 tcatctttgt atcgggagct tcctgaagac aggtgccgat catgatcttg aggttttccg
     2461 ccgtctgttc gccgatggcg aggttatact gcttgcgcag aaaacggata attccctcgt
     2521 cgaagcggtc gccccctata cgaagcgact tcgagacgac gatgcccccg agggagatga
     2581 cggcgatatc cgtagtgccg cctccgatgt cgacgaccat ttttccgcgg ggctcctcga
     2641 cgttgaggtt cgcgccgatc gccgcggcca tcggctcttc gatgaggtag gcttccttcg
     2701 cgccgacctc gactgcggct tcgagaaccg cgcggcgctc gacgtccgtg gcgcccgaag
     2761 gaacgcagat catgacccgg ttgcggaaga aacggttgaa gcccttgagc acacggcgca
     2821 tgaagtggtg catcatggct tcagtcatag tgtag
//
LOCUS       DAOSWW010000281         2853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26318_length_2853_cov_3.726948, whole genome shotgun sequence.
ACCESSION   DAOSWW010000281 DAOSWW010000000
VERSION     DAOSWW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26318_length_2853_cov_3.726948"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PK442_06545"
     CDS             complement(<1..248)
                     /locus_tag="PK442_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87342.1"
                     /translation="MERIGDSLSRGMDICSSKDGGAPMATPHVAGAVTTLDRRRVSGV
                     SSGLFRCPSLLRRCSAGWMPSSSRASSSKRPESRASTGR"
     gene            377..652
                     /locus_tag="PK442_06550"
     CDS             377..652
                     /locus_tag="PK442_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="HOO87343.1"
                     /translation="MDGKEMRKLLRKLLAKAWADAKFKARLIADPASVLRTEGMEVPE
                     GMKIHVVENTDDRLYLVIPRSPEELADEELDFLSAEGLSARRTAPDR"
     gene            complement(684..2159)
                     /locus_tag="PK442_06555"
     CDS             complement(684..2159)
                     /locus_tag="PK442_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOO87344.1"
                     /translation="MALLLCPASQATAVAMWNVTSGADDPSNPAVGTFRYTALLASPG
                     DTVRFTGAGTTVVLADQVLIDRTLTLNGPATIRQGAVNKRVLEVTATCTMNNITITGG
                     NFENQGLSALKGAGIYNRTTGVLTMNSCTLTGNTRGGAIENYGNLTMNSCTLTNNTNS
                     LAFGSAVTNAYLGGGLHASAALNGCTIENNDVAGSLGGAIYNGDILVMSNTKVCNNTT
                     GKGSGLQSRKGGGLYVGSRSNTTLTNGCRVTGNDPSQLYKEETYFSTPVFTYDSTCVI
                     GDAPNKSATAFSGYTGETAPEPRSIKDDADVAEVKRKLADSGSDLFATVSRALSSDLG
                     RTPDGTSATLAGLTASLYYANTFENVGVESQDLVVEYTASWPENARYYALFARADNAG
                     YELAERGIQFELKAGQSLPDGATPPDFYEEGEGLMTWRNVVTDGGSFDLEPAPGVVSF
                     RVCSVRAAEAQTASSGGSGCSALGASPFVLLLGLPLLLRKR"
     gene            complement(2429..>2853)
                     /locus_tag="PK442_06560"
     CDS             complement(2429..>2853)
                     /locus_tag="PK442_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HOO87345.1"
                     /translation="GEGKLEVEFTASWPKNVRYYAALAEYADAAAGARAVKGYVIPER
                     GVQFEIKPGQSLPGGMEPPDFYVEGEGLMTWRNVVEDNGPYDHNPQDGVVTFRVTSIR
                     AEAQTAPTGGSGCSSATASPFALLLGVPLLLLRKTRRI"
BASE COUNT          498 a          893 c          909 g          553 t
ORIGIN      
        1 cgcccagtag acgctcggct ctccgggcgc ttcgaagaag aagcccgaga cgacgacggc
       61 atccaccccg ctgagcacct cctcaagagc gatgggcagc ggaaaaggcc cgatgatacg
      121 ccggaaacgc gccgacggtc aagcgtcgtc accgcgccgg cgacatgggg cgtggccatg
      181 ggggcgccgc cgtcttttga agagcagata tccatgccgc gggagaggga gtctccgatt
      241 ctttccattc gggctgaatc acgaagcgct cttccatcgg cacggcgagg tagtcgtttc
      301 cgggtgagaa aacctactcc tcacgcagaa aaagcgtata attccgaata ggatatgtat
      361 tcgggaggga ttggctatgg acgggaaaga gatgcgtaag cttctgagga aactgctcgc
      421 caaggcgtgg gcggacgcga agttcaaagc gaggctgatc gccgatcccg cgtcggtttt
      481 gaggaccgag gggatggagg ttccggaggg catgaaaatc catgtggtcg agaacacgga
      541 cgaccggttg tatctggtca tcccgagaag ccccgaggaa ctggccgacg aggaactcga
      601 cttcttgtct gcggaaggcc tatcggcccg tcgaacggcg ccggaccgat aggcgaataa
      661 ttacggcgtc gtttagggat gtcttaccgt tttcgcagca gtagcggcaa tcccagaagg
      721 agcacgaaag gcgacgcgcc taaggccgag cagcccgaac cgccggagga ggccgtctgc
      781 gcctcggcgg cgcgcaccga acacacgcgg aagctgacga cgccgggcgc cggttcaagg
      841 tcgaacgatc cgccgtccgt cacgacgttc ctccaggtca tcagcccttc gccctcctcg
      901 tagaagtccg ggggcgtcgc gccgtcgggc aggctctgcc cggccttcag ctcgaactgg
      961 atgccgcgtt ccgcaagttc gtaccccgca ttgtcggcgc gggcgaagag cgcgtagtac
     1021 cgcgcgttct cgggccacga cgccgtgtac tcgacaacga gatcctgact ctcgacgccc
     1081 acgttctcga acgtgttcgc gtagtagagg gacgcggtca gacccgcgag cgtcgcgctc
     1141 gttccgtcgg gagttctgcc gaggtccgaa gagagggccc ggctgacggt cgcgaagaga
     1201 tcgcttccgg aatcggccag tttgcgcttc acttcggcga catcggcgtc gtccttgatc
     1261 gagcgcggtt ccggcgccgt ttctcccgtg tatcccgaga acgccgtcgc cgacttgttc
     1321 ggcgcgtcgc cgatcacgca cgtgctgtcg tacgtgaaga ccggcgtcga gaagtacgtc
     1381 tcctccttat agagctgcga cggatcgttt ccggtcactc ggcatccgtt cgtgagcgtt
     1441 gtgttcgagc gagaacccac gtagagtcct cctcccttgc ggctctgcag cccgctgccc
     1501 tttccggtcg tgttgttgca aactttggtg ttgctcatga cgaggatgtc cccgttgtag
     1561 atcgcgccgc cgaggctccc cgcgacgtcg ttgttttcga tcgtgcagcc gttcagcgcg
     1621 gcgctcgcat ggaggccgcc gccaaggtac gcgttcgtga cggcggaccc gaacgccaag
     1681 gagttcgtgt tgttcgtcag cgtgcacgag ttcatcgtca aatttccgta gttctcgatg
     1741 gcgccgccgc gcgtgttgcc cgtcagcgtg cacgagttca tcgtgaggac tcccgtcgtg
     1801 cggttgtaga tcccggcccc cttcagcgcc gacaacccct ggttctcgaa gttgcctccc
     1861 gtgatcgtga tgttgttcat cgtgcacgtc gccgtcactt cgaggacgcg cttgttcacg
     1921 gctccctgcc ggatcgtcgc ggggccgttg agcgtgagcg tcctgtcgat gagcacctgg
     1981 tccgccagaa cgaccgtcgt tcctgcgccg gtaaaccgga cggtgtcccc cggcgaagcg
     2041 aggagcgccg tgtaccggaa ggttcccaca gcgggattcg acgggtcgtc cgccccgctc
     2101 gtcacgttcc acatcgcgac ggccgtcgcc tgcgacgcgg ggcagagcag taatgccatc
     2161 accgttatca tcctcaaagc ccgaaaaccg aaacaacctc gttgcaccat agtcttcgct
     2221 tcctctcgtt gagtattcgc gttctgcgaa aaaagtatgc gtcgtaaagt aaagcaaatt
     2281 tgaattcgtt tatagacata aggaagcgga aggctggttg tacgcgtttc cgccttctag
     2341 gaggcgcgga tggcgccgga gaattctctc gcggcgccgc ttcccgtgcc gtcggtcgct
     2401 tccggcgagc gtcggttata gcgcgccttc agattcgccg tgttttccgc agaaggagca
     2461 gcgggactcc caacaagagc gcgaagggcg acgcggtcgc tgacgagcag ccggagccgc
     2521 ctgtgggagc cgtctgggcc tcggcgcgga tggaggtcac tcggaaggtc accacgccgt
     2581 cctgcgggtt gtggtcgtac ggtccgttgt cctccacgac gttccgccac gtcatcagcc
     2641 cttcgccttc cacgtagaag tcgggaggct ccatgccgcc gggaaggctt tgccccggtt
     2701 tgatctcgaa ctgcacgccg cgctcgggga tgacgtaccc cttcacggcg cgggctcccg
     2761 cagcggcgtc cgcgtactcg gcaagcgcgg cgtagtagcg gacgttcttc ggccaggagg
     2821 ccgtgaactc cacctcgagc ttcccctctc cgg
//
LOCUS       DAOSWW010000282         2853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26325_length_2853_cov_3.022516, whole genome shotgun sequence.
ACCESSION   DAOSWW010000282 DAOSWW010000000
VERSION     DAOSWW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26325_length_2853_cov_3.022516"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PK442_06565"
     CDS             complement(<1..683)
                     /locus_tag="PK442_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOO87346.1"
                     /translation="MDIGISKRLAKLYEEGKEAVLCMVVEESGSTPRSMGSSMLVFPG
                     GKIEGTVGGGITEHRVIEKALELLGKGTGSMMYRETLSATEAALDGAACGGSMGIYLE
                     VIGRKRELVIFGAGHVGKAIARLGDMTGFAVTIWDERAEFANEENIPWGRTVCCPLEN
                     FFDHVAPFHGDTYVIIVTRGHVLDADVVKLVEGMTAAYMGMIGSRKKIAFVRERLLGQ
                     GVSAGHIDR"
     gene            complement(683..1789)
                     /gene="mnmA"
                     /locus_tag="PK442_06570"
     CDS             complement(683..1789)
                     /gene="mnmA"
                     /locus_tag="PK442_06570"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOO87347.1"
                     /translation="MNIRPSTGFSSSSPSFGQGTVVVLMSGGVDSSVTAWLLQKAGWH
                     VVGVTMKIPGADLSPGSCCGRDAASVALGLGIPHYTAFIEREFGQLVIEPFVESYLRG
                     STPNPCADCNALLKFGLLWDELEDAFGETRLATGHYARVEQEGERFFLSRGKDPSKDQ
                     SYFLYGVARRRLPRLLLPLGGYSKEDVRALAREAALPVAEKSESMEICFAGEGDYRRL
                     LDGRSLPPGLIVDEDGEPLGAHRGIANYTVGQRKGLGISSRDGLHVLRIDPGRNAVIV
                     GPRERAFRRRVRASNVNILFPEPPAKGALLFGKTRSRGEPLPCDLLRMDENVLEVLFH
                     EPQFAPTPGQRLVLYDAGGRVAAGGVIEYSEEAE"
     gene            1878..2708
                     /gene="larE"
                     /locus_tag="PK442_06575"
     CDS             1878..2708
                     /gene="larE"
                     /locus_tag="PK442_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOO87348.1"
                     /translation="MPYHAAMKKMERVEALLREMGSAAVAFSGGVDSTLLAAAASRAL
                     GERAVAVTAASPTLPSWEIDDAEQYAALIGIRHVSIPLSELEEPRFAANDRDKCYYCK
                     RFRFELLLKWAKKEGFRWVLDGSNTDDLNDYRPGMRALSELEGVRSPLLEAGLSKSEI
                     RAVSKQWGLPSWQKPAAACLASRIAWGVPITAERLRQVERAEEIVRSFCPPNTQIRVR
                     HHGALARIETPPSALSLLAAPETAARLAPAVLALGFSFVTLDLSGYKMGSLNALFTDG
                     "
BASE COUNT          547 a          925 c          827 g          554 t
ORIGIN      
        1 atccggtcga tgtgcccggc ggagactccc tgtcccaaca gccgttcgcg cacgaaggcg
       61 atcttctttc tggagccgat catccccatg tacgcggcgg tcattccttc gaccagcttc
      121 acaacgtccg cgtcaaggac atgccctcgc gtaacgatga tgacgtaggt gtccccgtgg
      181 aaaggggcga catgatcgaa aaaattctca agcggacagc agacggtcct tccccacggg
      241 atgttctcct cgttggcgaa ctccgctctt tcatcccaga tggtgacggc gaatccggtc
      301 atgtccccga gacgggcgat cgctttcccg acgtgccctg ccccgaagat gacgagttcg
      361 cgctttctcc cgatgacttc gaggtatatt cccatggaac ctccgcaggc cgccccgtcg
      421 agcgcggctt ccgtagcgga gagcgtctct ctgtacatca tgctgcctgt tcctttgccg
      481 agaagttcga gggctttttc gatgacgcgg tgctcagtga taccgccgcc gactgtccct
      541 tctatctttc ccccgggaaa gacgagcatc gaggatccca tgcttcgggg agtcgacccg
      601 ctctcctcca ccaccataca gagcaccgct tcttttcctt cttcgtagag cttcgccagc
      661 ctcttcgaaa tgccgatatc cattattccg cctcctcgct gtattcaatg acgccccccg
      721 cggccactct gccgccggcg tcgtagagta ccagccgctg ccccggagtc ggggcgaact
      781 gcggctcgtg aaataagacc tcaagcacgt tctcgtccat gcggagcaga tcgcacggaa
      841 gcggctcccc ccgcgagcgg gtcttgccga aaaggagcgc ccctttcgcc ggcggttcgg
      901 ggaagaggat attcacattg ctcgcgcgta cgcgccgcct gaaagccctc tcccgaggac
      961 cgacgatgac ggcgtttcgc ccgggatcga tccgcagcac gtgcagtccg tcccgcgagg
     1021 agattccaag cccttttctc tgccctacag tgtagtttgc tatccccctg tgcgcgccga
     1081 gtggttcgcc gtcctcgtcc actatcagtc ccggcggaag cgaacggccg tcgagcagtc
     1141 ggcggtaatc cccctcgccc gcgaaacaga tctccatact ctcgctcttc tcggccacag
     1201 gaagggcggc ctccctcgcg agagcgcgca catcctcctt ggaataccct cccagcggaa
     1261 gcagaagtcg cggaaggcgg cggcgcgcca ctccatagag aaaatagctc tgatccttgg
     1321 aagggtcctt tcctctggag agaaagaagc gctctccctc ctgttctacg cgcgcatagt
     1381 gcccggtggc gaggcgcgtc tctccgaacg catcctccag ctcgtcccac aggagaccga
     1441 acttcagcag cgcattgcag tcggcgcatg ggttgggagt gctcccgcga aggtagctct
     1501 ccacgaacgg ttcgatgacg agctgtccaa actctctttc gataaaggcc gtatagtgcg
     1561 gaattccgag cccgagggcc accgacgccg catcgcgtcc gcagcagctt ccgggcgaca
     1621 aatccgctcc cgggatcttc atggtgacac ccacgacatg ccacccggct ttttgcagca
     1681 gccaggccgt cacggagctg tccacgccgc cgctcatcag gacgacgact gtgccctggc
     1741 cgaaggaggg cgatgaggag ctgaatccgg tcgatggtcg aatgttcatg aggaacttcc
     1801 ctccgggcat tggaatgtct tctcttctca tgtagtatag agaagaacaa agttcttcac
     1861 aagggaggat cgagagcatg ccgtaccacg ccgcgatgaa aaagatggag agagtggagg
     1921 cattattacg ggagatggga agcgcagccg tggcgttttc gggcggagtg gacagcacgc
     1981 tcctcgccgc agcggcttct cgcgcccttg gagagagggc cgtggccgtc accgccgcgt
     2041 cgccgacgct tccttcatgg gagatcgacg acgcagagca gtacgccgct ttgatcggca
     2101 tccgccacgt gagcattccg ctctcggaac tggaggaacc gcgctttgcc gcaaacgaca
     2161 gggacaagtg ctactactgc aagcgattcc gtttcgagct gctgctcaag tgggcgaaaa
     2221 aagaggggtt ccgctgggtg ctcgacggct ccaacaccga cgacctgaac gactaccgtc
     2281 cgggaatgcg cgccctctcc gagcttgagg gggtacggag cccgcttctc gaagccggat
     2341 tgagcaaatc ggagatacgc gccgtctcaa aacagtgggg gcttccgtcg tggcagaaac
     2401 ccgcggcagc ctgccttgcg agccggatcg catggggcgt gcccatcacc gcggagcgct
     2461 tacgccaggt agaacgggcg gaagagattg tacgctcctt ttgccccccc aatacgcaaa
     2521 tcagggtccg tcaccacgga gcgctggcga gaatcgaaac tccgccctcc gcgctctcgc
     2581 tgctcgccgc gccggagact gcggcacggc tcgcgcccgc tgtgctcgcc ctcggctttt
     2641 ctttcgtcac cctcgatctc tccggctata aaatggggag tctgaacgcg ttatttactg
     2701 acggctaacc gctcgggaac gctccatacc ccgtcttcgt acacttcaag agcgcaggtg
     2761 tcgatgtgga aaaacaagag ggaggccgat acggagcctt cgtcggccat cttcttgaaa
     2821 agcggatact gctcaacgcg gcgaagctgg cgg
//
LOCUS       DAOSWW010000283         2851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26369_length_2851_cov_2.535408, whole genome shotgun sequence.
ACCESSION   DAOSWW010000283 DAOSWW010000000
VERSION     DAOSWW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26369_length_2851_cov_2.535408"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(481..909)
                     /locus_tag="PK442_06580"
     CDS             complement(481..909)
                     /locus_tag="PK442_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOO87349.1"
                     /translation="MNRLTPFLFFAAGLSADVLSKEWALRTLPPPTLHAGGDFFALAL
                     HFNRGAAFSLLRSVPGAALLLSVAGVCLLAAMYRSLPAFRRSPAWGLLFAGAAGNMAD
                     RLLHGYVVDWFRAGPLHMNAADILLCAGGALLMLQMLRKK"
     gene            complement(941..>2851)
                     /locus_tag="PK442_06585"
     CDS             complement(941..>2851)
                     /locus_tag="PK442_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOO87350.1"
                     /translation="IKGWLPDGMLLPDELLTDEWVVNDLLDIYGRSLLTGVRTIQGER
                     VFAAIRLDMNELDVFGDHALLPVISDSLGQIVWMGATQETPALSKHLKARGFILRKQI
                     PDQRWAIALSHQGSRVMMRQEQLPYGFTFTIAYPLASLMISSLSGLAVAAMLFLFALL
                     VILLLWALWNRGVYKSIATIANLADEMGDRLSQIDARDPLRGAETLFTLTKRFSGFKE
                     TFVRETNSFVRDLRNLFEVILRQQEELTAFNEETEAMNQELEQVNNQLISREALWERT
                     LEFSRAFARNQDVDAAISSTLDTLRRDVKAFGVLLSSVEGNSFRLTAWSGYNGELTNF
                     VVSKDGIAASESIISRSPVWVEDVRAHPGAHPVHPSVKSELLIPLFQAGEEEGVLEVA
                     FDRPMRRDSFLIETLTPVASFLGGLVHGEKMRRELKASYAYLAEKLQFVTGIYHDKTE
                     AHIARIGEYCKLLAREIGRDASEQEDIALFARLHDIGKLRIPYGILVKPGALDSDEFN
                     QIKRHPQWGADILGDAAWLQMARNICLTHHEKWDGSGYPRGLRGEEIPWEGRVATIAD
                     IYDALRSSRTYKPAMSHELAGSILLEGDERVRPEHFDPKVLTAFRRISGKFEEIFDSN
                     CDERDSPDCFLA"
BASE COUNT          509 a          888 c          928 g          526 t
ORIGIN      
        1 gcttgcaacg ttgatccggt agagcgtgtc caggtcttgt ccgtagacgg acgctccgtc
       61 cgcagaccag aagggggagt agatcccctt ttgctccggc gcgaggacgc ggaagctccc
      121 gtccgctatc gtcgctaccc cgaacaccca gtcggaactc cgctcgtccc agtggttgaa
      181 gaggagcgac ttcccgtccg gcgaccagcg cgggccgtag ctgttctcgc cggggatcgt
      241 cttcaggacg gttgctttct tcgacgccgc ctcgacaacg ccgcgcgcac tcccctggaa
      301 atcatcgcta ccgcctcctt catgaatgat tgaacggacg tccgctgtgg acagccgtta
      361 ccctcggatg caggttatgc acttgttgtg gatacgttgt ccacaataaa gcgtctgttt
      421 tccccttttc tatgatcgta tcgcccggaa acactgcgca gcgtgcggcg tttttagaag
      481 ttactttttc cgcagcattt ggagcataag caacgcgccg ccggcgcaga ggaggatgtc
      541 ggcggcgttc atgtggaggg ggcctgcgcg gaaccagtcg accacgtagc cgtggagcag
      601 cctgtccgcc atgtttcccg ccgcgcccgc gaagaggagc ccccaggcgg gggagcgccg
      661 gaaggccggg agcgacctgt acatcgcggc gagcaggcag acgcccgcca cgctcaacag
      721 cagcgcagcg ccggggacgg agcggagaag ggagaatgcg gcgccccggt tgaagtggag
      781 ggcgagcgcg aaaaaatccc ctcctgcatg gagcgtcgga ggggggagcg tccgaagggc
      841 ccactccttg gagagcacgt ccgcggaaag tcccgcggcg aaaaaaagaa acggcgtcag
      901 gcggttcatg atgtgcgagg cgcccgggtc atcttctccg ctacgcgagg aagcagtccg
      961 gcgagtcgcg ctcgtcgcag ttggagtcga agatctcctc gaacttcccg gagattcgcc
     1021 tgaacgcggt gagcaccttc gggtcgaagt gctccgggcg caccctctcg tccccctcaa
     1081 ggaggatact gcccgccagt tcatgcgaca tcgcgggctt gtacgtgcgg gacgaccgga
     1141 gcgcgtcgta gatgtccgcg atggtggcca cccgcccctc ccagggaatc tcctcgccgc
     1201 gcagtccgcg ggggtatccg ctgccgtccc acttttcgtg gtgcgtcagg cagatattcc
     1261 tcgccatctg cagccaggcc gcgtcgccga ggatatcggc tccccactgc gggtggcgct
     1321 tgatctggtt gaactcgtcc gagtcgagcg cccccggttt caccaggatg ccgtacggaa
     1381 tccgcagctt gccgatgtcg tgcagacggg cgaagagggc gatgtcctcc tgctcgcttg
     1441 cgtccctgcc gatctcgcgc gcgagcagct tgcagtactc gccgatgcgg gcgatatgcg
     1501 cctccgtctt gtcgtggtaa atccccgtga cgaactggag cttttccgcg aggtaggcgt
     1561 aggaggcctt cagctcgcgg cgcatcttct ctccgtgaac cagtccgccg aggaaggagg
     1621 ccaccggagt gagcgtttcg atgaggaagg agtccctccg cataggccgg tcgaaggcga
     1681 cttcgagcac gccctcctcc tcaccggcct ggaagagcgg gatcagcagc tcgctcttga
     1741 ccgaagggtg caccggatgg gccccggggt gcgcgcggac gtcctccacc cagacgggcg
     1801 accgggagat gatgctctcc gaggcggcga tgccgtcctt cgagacgacg aagttcgtga
     1861 gctctccgtt gtagccgctc catgcggtca gccggaagct gttcccctca accgaggaga
     1921 ggagcacccc gaacgccttc acgtcccttc gcagcgtatc gagcgtggag gagatcgccg
     1981 cgtccacgtc ctggttgcgg gcgaaggcgc gggagaactc cagagtgcgc tcccacaggg
     2041 cttcccggct tatgagctgg ttgttcacct gttcgagctc ctggttcatc gcctcggtct
     2101 cctcgttgaa cgccgtcagc tcctcctgct ggcggagaat gacctcgaag aggttgcgga
     2161 ggtcgcggac gaaggagttc gtctcccgga cgaacgtctc tttgaagccg gagaagcgct
     2221 ttgtaagggt gaagagcgtc tccgcgcccc gcagcggatc cctcgcgtcg atctgcgaaa
     2281 gccggtcgcc catctcgtcg gcgagattcg cgatggtcgc gatgctttta tacacgcctc
     2341 tgttccagag agcccagaga agcaggatga caaggagagc gaagaggaag agcatggccg
     2401 cgacggcgag accggagagc gacgagatca tgagcgatgc aagcgggtag gctatggtga
     2461 aggtgaatcc gtagggaagc tgctcctggc gcatcatcac gcgggagccc tggtgcgaaa
     2521 gggctatcgc ccatcgctgg tcgggaattt gcttcctgag gatgaaccct ctcgccttca
     2581 agtgcttcga gagcgcgggg gtttcctgcg tcgcacccat ccagacgatc tgtcccaggc
     2641 tgtcggagat gaccggcaga agcgcgtggt cgccgaagac atccagttcg ttcatgtcga
     2701 gacggatcgc cgcgaagacc ctttctccct gaatcgtgcg caccccggtg agaagactgc
     2761 ggccgtaaat gtcgagcagg tcgttcacga cccattcgtc tgtcagcagt tcgtccggga
     2821 gcagcatccc gtccgggagc caccccttga t
//
LOCUS       DAOSWW010000284         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_26654_length_2830_cov_4.106667, whole genome shotgun sequence.
ACCESSION   DAOSWW010000284 DAOSWW010000000
VERSION     DAOSWW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26654_length_2830_cov_4.106667"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PK442_06590"
     CDS             complement(<1..526)
                     /locus_tag="PK442_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87351.1"
                     /translation="MDCYWYISDSLRPWEAAVVGTLARALIREGVKLHSFCGGGTESL
                     SIPGLLSWHSLNATERALIVGTKGKLWHLWGTPPSWWKFIRLRSRTIHTLFENPAKDW
                     KGHPTVLSATACSSGESYIPPAFEVKMNWSADEGKESFEETGTPVFLLSGGSAEEFEV
                     FAEGAAKASSTLRNF"
     gene            complement(556..1836)
                     /gene="tyrS"
                     /locus_tag="PK442_06595"
     CDS             complement(556..1836)
                     /gene="tyrS"
                     /locus_tag="PK442_06595"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOO87352.1"
                     /translation="MFSDALHVLKERGHVEWCSNEEELHSLFTSEMVTAYVGFDPTAD
                     SLHVGHLIPIMALAWIQRCGHRPIPLAGVGTGLIGDPSGKSKERNLLTIEKVQENVIG
                     VKKQLARFLDFDCGGASAQIINNYDWLGKLTFLDVLREVGKHFSVNSMVSREYVKSRL
                     DDPEKSISYTEFSYVLLQAYDFLHLFQNYGCRLQMGGNDQQGNIISGIDLIRKKTGGE
                     AFGATQPLLLTSSGTKFGKTEEGAVWLDPQKTSPYKFYQFWMNTEDQAVEKLLKLFTF
                     LSLEEIEEIMQVHRSAPERREAQRRLAWEVTGIVHGQDNAATTKKASGILFGESFEPR
                     ELSLSMLDTLAAEVPSGTVSRVFPALLTDILAEAGACSSKSEVRRLLKGGGLSVNGVK
                     VTEEDASVSEDGILAGKYLFVRLGKKRFFMILFQ"
     gene            complement(1890..>2830)
                     /locus_tag="PK442_06600"
     CDS             complement(1890..>2830)
                     /locus_tag="PK442_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87353.1"
                     /translation="WKLERRWPNHIFFSDGGFFSGIASMEGLPAAKENLLVTAAWRDE
                     DDGGRMEWHLEGLDTIFPPSLLKEFREAHWGGRYTFPEPLVAAVGFTIPERFAAFLED
                     GRMSEAFGLWENADELIKGLLAGPCILSLCGSSKFLVYSLPGVLLQLPERGREGETFA
                     EAFWKKEWSALVPGVEKLDGFPVGGTTAIPFSIVAAANFRMLQLGLIDRDALRHERFR
                     SIYDYAPLLSDIENAFFWSYLDIPKIASALQDLAKIGQVAAKMGKNLRVSPNALFQAA
                     DALKEMGGVTIVMPVPEEGFLEWRITKDAAPTEEME"
BASE COUNT          665 a          794 c          673 g          698 t
ORIGIN      
        1 caaagtttcg gagagtgctg gacgctttcg ccgctccttc tgcaaacacc tcaaactctt
       61 cggcgctacc tcccgagaga agaaaaacag gggtcccggt ttcttcaaag gattcttttc
      121 cctcgtctgc gctccagttc atcttcactt cgaaggcagg cgggatatag ctttctcctg
      181 aagaacatgc cgtggccgag agtacagtgg gatgcccctt ccagtctttc gccggatttt
      241 cgaaaagcgt atggattgtc ctcgatcgta acctgatgaa tttccaccac gagggcggag
      301 ttccccaaag gtgccacagc tttcccttcg tgccgacaat gagcgcacgc tctgtagcgt
      361 tcaacgaatg ccacgagagt agaccgggaa tactgagcga ctccgttcct cctccgcaga
      421 atgaatggag cttgacgcct tcccgaatca acgctctcgc gagagttccg acaaccgcgg
      481 cttcccacgg gcgaagagag tcgcttatgt accagtagca atccatcagg tatcccctcc
      541 ccttttaccc ggctcctatt gaaaaagaat catgaagaag cgtttctttc caaggcgaac
      601 gaagaggtac ttcccggcga gaataccgtc ctcggaaaca gacgcgtctt cctccgttac
      661 ttttactccg ttgacggaga gccctccgcc tttgaggaga cgccgtactt cgctcttcga
      721 agaacatgcg cccgcttcgg cgagaatatc ggtcagcagt gcggggaaca cgcgggatac
      781 cgttcccgaa gggacttcgg cagcaagagt atcaagcatg gagagtgaaa gctcccgggg
      841 ttcgaacgac tctccgaaga ggattccgct ggcttttttc gtcgttgcgg cgttatcctg
      901 tccatggacg atgccggtta cttcccaggc cagccttcgc tgcgcttcac gacgttcggg
      961 agcgcttcgg tgcacctgca ttatctcctc tatttcttcg agagagagga atgtgaaaag
     1021 cttcaggagc ttttccacag cttggtcttc agtattcatc cagaactggt aaaacttgta
     1081 gggggacgtt ttttgaggat caagccagac tgcgccctct tcagtttttc caaatttcgt
     1141 tccagatgaa gtcagaagga gcggttgcgt tgctccgaaa gcttctcctc ctgttttctt
     1201 ccggatgagg tcaatgccgg agatgatatt tccctgctgg tcattccctc ccatctgtaa
     1261 acggcacccg tagttctgga ataggtgcag gaagtcgtag gcttgtagaa gaacatacga
     1321 aaactccgta taggaaatgc tcttctccgg atcgtcgagc ctgcttttta catactccct
     1381 tgagaccatg gaattgacgg agaagtgttt acccacttct ctgagaacat ccagaaacgt
     1441 cagcttccct agccagtcat agttgttgat aatttgagcg gatgcccctc cgcagtcgaa
     1501 atcaaggaag cgagcaagct gttttttcac tccaatcacg ttttcctgca ccttttcgat
     1561 agtgaggaga tttcgttctt tgctctttcc cgaaggatct ccaatcaatc cggtaccaac
     1621 ccctgcaaga gggatcggcc gatggccgca tcgttgaatc caggcaagag ccatgatcgg
     1681 aatcaggtgg ccaacgtgca ggctgtcagc cgtaggatca aaccccacat acgccgttac
     1741 catctcgctt gtgaaaagag agtgcaactc ttcttcgttg ctgcaccatt cgacatgtcc
     1801 tctttctttg agaacatgaa gcgcgtctga aaacatgtac ttcccctccc gcttttctgt
     1861 aatgaaccgc aatccgccga tagtgaattc tactccattt cctcagttgg tgcagcgtct
     1921 tttgttattc tccattcgag gaatccttct tcggggacag gcataacgat tgttacgcct
     1981 cccatttctt ttagggcatc ggcggcttga aaaagcgcat tcggactcac tctcaagttc
     2041 ttgcccattt ttgccgctac ttgacctatt tttgcaagat cctgaagcgc cgaggctatc
     2101 ttcggaatat ctaaataaga ccagaaaaaa gcattttcaa tgtccgaaag taacggcgca
     2161 tagtcataga tcgaacgaaa tctctcgtgc cgtaacgcgt ccctgtcgat caatccgagc
     2221 tgaagcatac gaaaatttgc agcggccact atggaaaaag ggatggctgt agtgcctcct
     2281 accggaaatc catccaactt ttctaccccg ggaacgagag cgctccattc tttcttccag
     2341 aaggcctctg caaatgtttc tccttcccgc cctcgttccg gtagctgcag aaggactcct
     2401 ggaagggaat aaacaaggaa cttactgctg ccgcacaagg aaagaataca aggccccgca
     2461 agcagcccct tgatcagttc atccgcattt tcccacagac cgaaagcctc gctcatgcgg
     2521 ccgtcctcga ggaaggccgc aaacctctcg gggatcgtaa atccgactgc agcgacgaga
     2581 ggctcgggga aagtataacg ccctccccag tgagcttccc gaaactcttt cagcaaggaa
     2641 ggggggaaaa tagtatcaag cccttccaaa tgccattcca tgcgtccgcc gtcatcttcg
     2701 tctctccatg ccgctgttac gaggaggttt tctttagctg caggcagccc ctccatcgag
     2761 gcaatcccgg aaaaaaaccc tccatcggaa aagaaaatgt gattaggcca ccgtcgttcc
     2821 aatttccagg
//
LOCUS       DAOSWW010000285         2806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27014_length_2806_cov_3.405671, whole genome shotgun sequence.
ACCESSION   DAOSWW010000285 DAOSWW010000000
VERSION     DAOSWW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27014_length_2806_cov_3.405671"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PK442_06605"
     CDS             complement(<1..428)
                     /locus_tag="PK442_06605"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOO87354.1"
                     /translation="MSVFDNDASTYDQWYTTPMGRHVDDVETSCALALLQPFSGMKIL
                     DVGCGTGVLSLAMYSQYPDADILAVDRDALALAFTEMNCRENGFQITTASSLGCAGVF
                     DRTFDLVMSNIPAKAGLPVIATMLTDFSRLAGPKGTAAVV"
     gene            complement(520..1392)
                     /locus_tag="PK442_06610"
     CDS             complement(520..1392)
                     /locus_tag="PK442_06610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO87355.1"
                     /translation="MTNSEFSLLIGYRDIDPARFSFNELARKTFGLDFERWYRSGYWT
                     KQYIPYSLAKEGQVVANVSVSFMPVNVDGAPMTMAQIGTVMTDPAFMGRGLCRILMET
                     VLEEWTPKVDDVYLFANDATLDFYPKFGFRKSAEACASAAVRTSGPARMQKLDMDSAE
                     NLAKVERAVRGTRGVSRLRVNNAGLVMFHLTGRLRENVYSDPLTNAIIVAEPEGENLR
                     VDDVFCEDAVNLDDAIGAFGERVKRAAFGFPPTQDENMERTRCTEVNTTLFVLKNAVD
                     FFEKGLMFPVLSRA"
     gene            complement(1426..2301)
                     /locus_tag="PK442_06615"
     CDS             complement(1426..2301)
                     /locus_tag="PK442_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO87356.1"
                     /translation="MNAHYVKFISACLLFGSNGIVASHILMSSHEIVFLRTLIGSLFL
                     LAVFVLTGGKFSGLRNVRHLAYLILSGVAMGASWIFLFEAYQRIGVGMATLAYYCGPV
                     IVMALSPLLFKEKLTPARICGFIAVLLGMFLVNRDALMVDGLSWGLLCGVLSAVMFAL
                     MVILNKMAASITGFENAVWQLATSFVTVAIYTVLRQGFAIDIPSGSFAPVLLLGLVNT
                     GVGCYLYFSSMQRLPAQSVAICGYLDPLSALVFAAVFLAERMSPEQMAGAALILGGAA
                     FGEYFGRKRLPDVSW"
BASE COUNT          659 a          875 c          717 g          555 t
ORIGIN      
        1 atgaccactg ccgcggtccc cttcggaccg gcaagacggg agaaatccgt cagcatggtc
       61 gctatgaccg gcaatcccgc tttcgccgga atgttggaca taacgaggtc gaatgtacgg
      121 tcaaaaaccc ctgcgcatcc caatgacgac gcggtggtta tttgaaaccc gttctcccgg
      181 caattcattt cagtaaacgc aagcgcgagc gcatcccggt ccacggcaag aatatccgcg
      241 tccggatact gtgagtacat ggcaagcgaa agcacaccgg taccgcaccc cacgtcgaga
      301 atcttcatac cgctgaacgg ctgtaaaaga gcgagagcac agcttgtctc cacatcatcg
      361 acatgccgcc ccatgggagt cgtataccat tgatcgtacg ttgatgcgtc attatcaaaa
      421 acgctcatcg gcgacctcct ttcgttccgt cacactccgc gctcttccgc aggatcgccg
      481 ttcagtctgt cgggcaacga gaaaaagaag acggttccct caggcacggg agaggacggg
      541 gaacatcagc cctttctcaa aaaaatccac cgcgttcttc aggacgaaga gggtcgtatt
      601 cacctccgta cagcgcgtcc tttccatgtt ctcatcctga gtcgggggaa agccgaacgc
      661 cgctcttttc actctctcgc cgaaagcgcc gatcgcatcg tccagattca ccgcgtcctc
      721 gcagaagaca tcgtcgaccc gcaggttctc cccctcgggc tccgcgacga taatagcgtt
      781 cgtcaggggg tcggagtaga cgttctcacg caaacgcccg gtaaggtgga acatgacgag
      841 cccggcgttg ttcacacgaa gcctgctcac gccgcgcgtt ccgcgtactg ctctctccac
      901 tttcgccagg ttttcggcgg agtccatatc cagcttctgc atccgcgccg gccccgacgt
      961 gcggacggcg gcggaagcgc acgcttccgc gctctttcga aatccgaact tcggatagaa
     1021 atcaagcgtt gcgtcgttgg cgaaaagata gacatcatcg accttaggcg tccactcctc
     1081 cagcacggtc tccataagga tccggcacag tccccgcccc atgaaggcgg ggtcggtcat
     1141 caccgtgcct atctgcgcca tcgtcatcgg cgcgccgtcg acgttcaccg gcatgaagct
     1201 caccgacacg ttcgcgacaa cctgcccctc cttcgccaga gaatagggga tgtactgctt
     1261 cgtccagtac ccagaacggt accatctttc gaagtcaagc ccgaaggtct tccgggcaag
     1321 ctcgttgaac gagaatcgtg cagggtcgat atcccggtag cctatcagaa gtgaaaactc
     1381 gctgttggtc atcgtcttct ccttgaacag acgaatttga cttattcacc aagaaacatc
     1441 cggaaggcgc ttcctgccga aatactctcc gaacgcggcg cctcccagaa tcagcgccgc
     1501 cccggccatc tgctccggag acatgcgttc ggctaggaaa accgcagcaa agaccagcgc
     1561 cgagagcgga tccagatagc cgcagatcgc gacggattgc gccggaagcc gctgcatcga
     1621 cgaaaagtac agatagcacc cgacgcccgt gttcacaagc cccagcagaa gaacgggcgc
     1681 gaaacttccg gagggaatgt cgatcgcaaa cccctgcctg agcactgtat agatcgccac
     1741 cgtgacgaag ctcgtcgcaa gctgccacac ggcattttcg aaccccgtga tgctcgcggc
     1801 catcttgttg aggatcacca tgagcgcgaa catgaccgcc gagagcacgc cgcacagcag
     1861 cccccatgaa agtccgtcca ccatcagcgc gtcccggttg acaagaaaca tccccagaag
     1921 aacggcgata aagccgcata tccttgcggg agtcagcttt tccttgaaca acaacggcga
     1981 gagcgccatc acgatgaccg gtccgcagta gtacgccagc gtcgccatcc cgacgccgat
     2041 cctttggtac gcctcgaaga gaaaaatcca gctcgcgccc atggcgaccc ccgacaggat
     2101 caagtacgcg aggtggcgca cattccgcag cccggagaac ttcccccctg tcaggacgaa
     2161 aacggcgaga agaaacagac tgccgatcag cgtccgcaga aagacgatct catgactgct
     2221 catcagaata tggctggcca cgatcccgtt cgagccgaac agcaggcacg ccgatatgaa
     2281 tttcacgtag tgtgcgttca ttacacttca cctctcttca agtcaaaaag ttcggagact
     2341 ccttagatac agcgctattc tctctcatat gctcgaatta tcgagtggcg ccatcaaaga
     2401 acttcttcag tgcatcttcg gcggttcacg gcacaaggcc cactctttaa tatgataatg
     2461 attatcagtt gcatgtttga ctgttttgtg gcatcatgtc aacagaacac atagctccat
     2521 ttttcgggtc agaatgcgcg gaaaggctgc ttctccgctt taccggtttg cacaccattc
     2581 tacgcttttt aagacgcccg gtcatcttgt cgaggaggga atatagctgt gaatacaatc
     2641 gtcgaagagc ttatcgagta cggactctcc cattccaagg gagagcgcgt ggaagacgtc
     2701 cggatcggac tgggctatac cgcggtcaag cttgactccg caaaggccgg agttgcgtgc
     2761 atgctccgac atcgcctcgg aaagacaacc tgctcccttc tgccac
//
LOCUS       DAOSWW010000286         2794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27190_length_2794_cov_2.839357, whole genome shotgun sequence.
ACCESSION   DAOSWW010000286 DAOSWW010000000
VERSION     DAOSWW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27190_length_2794_cov_2.839357"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PK442_06620"
     CDS             <1..1085
                     /locus_tag="PK442_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOO87357.1"
                     /translation="LSFKISEGRSPVFKQLLKLTAPFSLALGAIAMVWVIEEQINITG
                     TLYLLLINVLTAVEWLGWTWVVVRAGELLAELIILSPRIESGSVDASLVRTLSRLASI
                     AAAIGILMFGASELGVPLMTLITGFGVAGLAVSLAARPTLENIIGGLILFTDRSVKVG
                     EFCRFGDKAGTVLHIGLRSTKIEALDQTVISIPNADFSQMQITNVSRRSKALLQKTIN
                     LRYETTRDQLRWILASIRDVLHAHPKIYADPAPFARFEGFGESSLDVLIFAYVKTKKW
                     SELMAIQEDILFRIGEIVEKSGSGFAFPSTTAYIAKDDGLDKEKGAAVEKEVQQWREE
                     DAYPFPDTPLERMSTLLGTIKYPPKA"
     gene            1390..>2794
                     /locus_tag="PK442_06625"
     CDS             1390..>2794
                     /locus_tag="PK442_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOO87358.1"
                     /translation="MAHISAGRALAPADRSSPRATMESFINHMNSGHLRLQRAFELSA
                     ADPSLFNHPQEALEEAGRALYDFERAMHCLDFSAIPQIVRLQEARESVLLLKEILDRV
                     GIPRYDQIPGAGEVAEQKLVRWTIPKTEIRLYRVSSGEEEGNWIVDAESVAELERAYR
                     RVRHLPYLPGATENAYYQFITTPGSFFPPKWVQRLPEWSTALVYEQTLWQWIALIAWH
                     LIFIAALSGAWILCRRLAERSTPFVSQALRIVPPVALALGTFLMGFIIESRLNITGAL
                     YLSLRNIFAVIQLMGWAWLIIRLAELIGEMIVFSPRVDPSGVNASLVRTISKLLGITA
                     AAGVIMHGASEMGFSLATILTGFGVAGLAVSLAAQPTIENIIGGIILFSDQSVKAGEF
                     CRFGDKAGTVLSIDLRTTKLEALDQTIISIPNAHLAKMQITNVSRRSKSLLQKTINLR
                     YETTRDQLRWILASIRDV"
BASE COUNT          573 a          867 c          767 g          587 t
ORIGIN      
        1 aactgagttt taaaatctcg gagggcaggt cgcctgtctt caaacaactc ctgaagctga
       61 ctgccccgtt ttcgctcgcc cttggagcaa tcgcaatggt ctgggtcatc gaagaacaga
      121 ttaacatcac cggaacgctc tatcttttgc tgatcaacgt ccttacagct gttgagtggt
      181 taggctggac gtgggtggtc gtccgggcgg gcgagctgct cgccgagctc attattctct
      241 cgcccagaat cgagtccggg agcgtcgacg ccagcctggt gcggacgctg agccgtctcg
      301 caagcatcgc cgctgccatc ggtattctga tgttcggagc ctccgagctc ggcgtcccgc
      361 tgatgaccct catcaccgga ttcggtgtcg ccggtttggc cgtctcgctg gcggcccgtc
      421 cgactcttga gaacatcatc ggaggcctca ttctcttcac cgaccggtcc gtcaaggtcg
      481 gcgagttctg ccgtttcgga gacaaggcgg gaaccgtact tcacatcggc ttgcggagca
      541 cgaagatcga agcgctcgat cagacggtga tctccattcc gaacgccgac ttctcgcaga
      601 tgcagatcac gaacgtcagc cgcaggagca aggcgctgct gcaaaagacc atcaaccttc
      661 gttacgagac gacgcgcgac cagctccggt ggatactggc ctcgattcgg gacgtgctcc
      721 acgcgcaccc gaagatctac gcggacccgg cccccttcgc ccgcttcgag ggcttcggag
      781 aatcatcgct cgacgtgctc atcttcgcct acgtcaagac gaagaagtgg tccgaactga
      841 tggcgataca ggaggacatc ctcttccgga tcggcgaaat cgtcgagaag tccggctccg
      901 gcttcgcctt cccctccaca accgcctaca ttgcgaaaga cgacggtttg gacaaggaga
      961 agggggccgc agttgaaaag gaggtgcagc agtggcgtga ggaggacgcc tacccgttcc
     1021 ccgacacacc gcttgagcgc atgtccacgc ttttagggac catcaagtac cctccgaaag
     1081 cctaaaaagt gcactgttca cgaaacggcc ctgatgttat gggccgtttc gcaccttttc
     1141 gtcaagcgtt caccaaagat gatgcgcacc ataaaaacag agctgtttca cagccgcgtc
     1201 attaccgcta aactcctgct atcgaaagag taccctgtga tctgaaaaga ggctgatcat
     1261 ccgatgaaag catctcgtta cgttgcgctt cgaattgtgt ttatagctct gtgcatactc
     1321 ttcgggaccc cttttctgct gtctgcatcc acggattcct cttcgcgtct ccgcctggaa
     1381 tggtccggaa tggcgcacat aagcgcggga agagcgcttg ccccggccga cagatcatcg
     1441 ccgcgagcaa caatggaatc cttcataaac cacatgaatt ccggtcatct gcgtttgcaa
     1501 agggccttcg agctctccgc cgccgatccg agtcttttca atcatccaca agaggcgctc
     1561 gaagaggccg gccgggcgct ctatgatttt gaacgtgcca tgcactgtct tgacttctcc
     1621 gccattcctc agattgtgcg ccttcaggag gcacgggaga gcgtgctgct tctaaaggag
     1681 atcctggacc gtgtcggcat cccgcggtac gaccagatac cgggcgcagg agaagttgct
     1741 gagcagaagc tcgtccgctg gacaattcca aagaccgaga tacgcttgta ccgggtctct
     1801 tcaggcgaag aggagggaaa ctggatcgtc gacgccgagt cggtagccga actggaacgc
     1861 gcctaccgca gggtgcgcca tcttccgtac ctgcccggag caaccgagaa tgcgtactat
     1921 caattcatca ccacccccgg gtcgtttttc cccccaaagt gggtgcagcg ccttccggaa
     1981 tggtccaccg cccttgtcta tgaacaaact ctctggcagt ggatcgccct gatcgcctgg
     2041 catctgattt tcatcgccgc tctgtcaggc gcctggatac tctgccggag actcgccgaa
     2101 cgctcgacgc ccttcgtcag ccaagcgctc agaatcgtcc ctccagtcgc tctcgctctc
     2161 gggacattcc tcatgggctt catcatagaa tcgcggctga acatcacggg ggcgctctac
     2221 ctctcgctga ggaacatctt cgcagtcatt cagctcatgg ggtgggcatg gctcattatc
     2281 cgtctggcgg agctgatcgg ggagatgatc gtcttctccc cgcgtgtcga cccctccggg
     2341 gtcaacgcaa gccttgtccg gacgattagc aaactcctcg gcatcaccgc ggcagcgggc
     2401 gtcatcatgc acggcgcctc tgaaatgggc ttctcgctcg caacgatcct caccggattc
     2461 ggagtcgccg gtttagcagt ctcgctggcg gcgcagccga ccattgagaa catcatcggc
     2521 ggcatcatcc tcttcagcga tcaatcggtc aaggcaggcg agttctgccg cttcggcgac
     2581 aaggcgggaa cagtgctctc catcgatctc cgcacgacga aactggaggc gctcgatcag
     2641 accatcattt cgatcccgaa cgctcacctt gcgaagatgc agattacgaa tgtcagccgc
     2701 cggagcaaat cgctgctgca gaagaccatc aacctccgct acgagacgac gcgcgaccag
     2761 ctccggtgga tactggcctc gattcgggac gtgc
//
LOCUS       DAOSWW010000287         2790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27260_length_2790_cov_4.239854, whole genome shotgun sequence.
ACCESSION   DAOSWW010000287 DAOSWW010000000
VERSION     DAOSWW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27260_length_2790_cov_4.239854"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PK442_06630"
     CDS             <1..429
                     /locus_tag="PK442_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOO87359.1"
                     /translation="VLPYASEGKENAERRLSGLCSARGVEWIVLAGFMRILSPEFVTA
                     HAGRIVNIHPSLLPSFPGRDGIGDAWRFGVAVTGVTIHLVDAGIDSGRILAQRAVEIF
                     PEDSLENLEEKIHTTEHALYWKTLSELFRTGVPAERRWRR"
     gene            426..1955
                     /gene="purH"
                     /locus_tag="PK442_06635"
     CDS             426..1955
                     /gene="purH"
                     /locus_tag="PK442_06635"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOO87360.1"
                     /translation="MKVRRALLSVYDKTGITELAKTLTDLGWELLSSSGTAEHLRKAG
                     LLVTEVADVTGYPHILGGRVKTLHPKVFGGILARRENGGDLKDVEEHDLPLIDMIVCN
                     LYPFEQAAKKHLPLDDLLENIDIGGVSLLRAAAKNYRQVVALMEPEDYPTVAEELRSE
                     GDVTLPTREQLAIKAFAGTASYDGMIVSGLKETTGYASPALPAKIPLILQKQQTLRYG
                     ENPHQEAALYLPALADLPWEQLSGKPLSYNNILDADCAMRGCALLQNCCGALVIKHTT
                     PCGMACGETPFEAYERAYGCDPVSAYGGVVGISRKVDMETVLALADRFTDVLVAPEYD
                     EETVALLAEKKPALRVLKWKGGRVLTDQITGTWSGLLVQADSLPPVPELEKGEWVGAP
                     RPDLWDDLLLAWKAAAISKSNAIALVKNGEAIGIGRGCCSRLHAVDFAVRQAGQKAKG
                     SVLGSDAFFPFTDGVELAADAGVAAIIQPGGSVRDKDVCAAVERRGISMFITGWRTFR
                     H"
     gene            1975..>2790
                     /gene="purD"
                     /locus_tag="PK442_06640"
     CDS             1975..>2790
                     /gene="purD"
                     /locus_tag="PK442_06640"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOO87361.1"
                     /translation="MTGKKNILVIGSGAREHALVHTLSRSGTVQEIHAAPGNPGMLPL
                     AKLHPVSDAACASILPLVKRHGIDLVVVGPEAPLVAGLADELRSAGVEVFGPGRAGAL
                     LEGSKVHAKKFMDRWGVPTAPWDECTSLQDVEEALAKRSAPFIVKADGLAAGKGVFVT
                     KTREEAFLAAENLLVRGALGEAGKRIIIEDALSGEELTVLAVSDGATYRILPPSQDHK
                     RVYEEDKGPNTGGMGAYSPVPWVGEELLERVRRSILYPTFDGLRHDGVPYCGVL"
BASE COUNT          607 a          793 c          836 g          554 t
ORIGIN      
        1 gttcttccgt acgcctcaga aggaaaggaa aacgcggagc gccgactgtc cgggctttgc
       61 tccgcccgag gcgtcgaatg gatcgttctc gccgggttca tgcgaattct gtctccggag
      121 tttgttacgg cgcatgccgg gcgtatcgtg aacatccatc cgtcgctcct cccgtccttc
      181 cccggaagag acggcatcgg cgacgcgtgg agattcggag tcgccgtaac cggcgtcacg
      241 attcaccttg tcgacgcagg tattgattcc ggccgtatcc ttgcacaacg ggcggtagaa
      301 attttccccg aagacagcct tgaaaacctt gaagaaaaaa tacatacgac cgaacatgct
      361 ctatactgga aaacgctttc ggaactcttc cgaacggggg ttccggctga aaggagatgg
      421 cgacgatgaa agtgcggcgc gcgcttctct ccgtctatga caagacgggg ataacagaac
      481 tggcgaagac gctgaccgac ctgggctggg aactgctctc cagttcgggg actgcggaac
      541 acctgcggaa agcagggctt ctggtaacgg aagttgctga tgtgacgggg tacccccaca
      601 tcttgggagg tcgggtaaaa accctccacc ccaaagtttt cggaggcatt cttgcccgga
      661 gagagaacgg cggcgatctg aaggatgttg aggaacacga tcttcccctt atcgacatga
      721 ttgtctgcaa cctctaccct ttcgagcagg cggccaagaa acaccttccg ctcgacgacc
      781 tgttggagaa tatcgatatc gggggagttt ccctgctgcg cgctgccgcg aagaactacc
      841 ggcaggtcgt cgcgcttatg gaacccgagg actacccgac cgtcgctgaa gaactgcggt
      901 cggaaggaga tgtaacgctg ccgacacgag aacagcttgc gataaaagcc tttgcaggaa
      961 ccgcatccta tgacggaatg atcgtttccg gtctgaagga gacgacaggg tacgcctctc
     1021 cggctctccc tgccaaaata ccgctcatac tccagaaaca acagacgctc cgatatggag
     1081 agaatccgca tcaggaagcg gcgctctatc ttcccgcact cgccgatctt ccctgggagc
     1141 agctctccgg caaacctctt tcgtacaaca atattctgga cgccgactgc gcgatgcgcg
     1201 gatgcgcgct tctccagaac tgctgcggag ctctggtgat caagcataca acgccgtgcg
     1261 gcatggcctg cggggaaact ccttttgaag cgtatgaacg tgcctacggc tgcgatcctg
     1321 tctccgcata cggcggagtc gtcggcatct ccagaaaagt cgacatggag accgttcttg
     1381 ccctggcaga ccggtttacg gacgtgctcg tcgctccgga gtacgatgag gagaccgtcg
     1441 ctctgcttgc ggagaaaaaa ccagcccttc gcgtgctcaa atggaaggga ggcagagtgc
     1501 ttaccgacca gattaccgga acgtggagcg ggctgcttgt ccaggcagat tctctgccgc
     1561 ctgttccgga actcgaaaag ggcgaatggg tgggcgcacc ccgtccagat ctttgggatg
     1621 acttgctcct cgcgtggaag gccgccgcga tttcaaagag caacgccatc gctctcgtca
     1681 aaaacggcga agccatcggc atcggcagag ggtgctgcag caggctgcac gcggttgatt
     1741 tcgcggttcg tcaagcaggt cagaaggcga agggatccgt gctcggttcc gatgcattct
     1801 tcccatttac cgacggagtc gaactcgccg cagatgcggg tgtcgccgca atcatccaac
     1861 ccggcgggtc cgtgagggac aaagacgtat gcgccgcagt cgaacggcgg ggcatctcca
     1921 tgttcattac cggatggcgt actttccgcc actgatgaaa gagacgatat cgctatgacg
     1981 ggaaagaaga atatcctcgt tatcggaagc ggcgcacggg agcatgctct cgtgcatacg
     2041 ctttcccgct ccggaaccgt ccaggagatc catgcggctc cgggcaaccc cggtatgctg
     2101 cctttggcaa aactccaccc cgtcagtgat gcagcatgcg catcgatact tcctcttgtg
     2161 aagcggcacg ggattgatct tgtggtcgtc gggccggagg cgcctctcgt cgccggtctg
     2221 gccgacgaac tccgctcggc cggcgtcgag gtcttcggcc cggggcgtgc gggcgcgctt
     2281 cttgaaggga gcaaagtcca cgcgaaaaaa ttcatggaca gatggggagt tccgaccgct
     2341 ccctgggatg aatgcacttc gcttcaagac gttgaagaag cgctggccaa acgaagcgcc
     2401 ccttttattg tcaaggccga tggtcttgcc gcaggaaaag gcgtatttgt aacaaaaacg
     2461 cgtgaggaag cgttcttggc ggcggagaac ctgcttgtac gaggcgcgct cggcgaggcg
     2521 ggcaaacgca ttatcattga agacgcactc tcaggagagg aactcacagt tttggccgtg
     2581 agcgacgggg caacctacag gattctgcct ccgagccagg accataagcg cgtctacgaa
     2641 gaagacaaag gaccgaatac aggcggtatg ggtgcatact cacccgttcc ctgggtcggc
     2701 gaagagcttc tggagagggt gcggaggagc attctctacc ctacgttcga cggtcttcgt
     2761 cacgatggcg tgccctactg cggcgttctt
//
LOCUS       DAOSWW010000288         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27351_length_2785_cov_3.317216, whole genome shotgun sequence.
ACCESSION   DAOSWW010000288 DAOSWW010000000
VERSION     DAOSWW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27351_length_2785_cov_3.317216"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK442_06645"
     CDS             <1..372
                     /locus_tag="PK442_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOO87362.1"
                     /translation="AESVRAGGYSLNSFFEAQAVKNPADEIFDTKAQDASYTVAFTLL
                     IFDGVTNDLSRLVQPLEGAGIIVFDGLKDGKYADPLILAAPRPKNAQTQGTSGCATSA
                     GGISPALLLLPGALLLLRRRK"
     gene            complement(436..1107)
                     /gene="prxU"
                     /locus_tag="PK442_06650"
     CDS             complement(436..1107)
                     /gene="prxU"
                     /locus_tag="PK442_06650"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048998.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(913..915),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOO87363.1"
                     /translation="MGAEAAPAEEVKKEAVRMGVTVGMKAPDFTAPAFYKGGFTTVKL
                     SQFAGKWVSLCFYPGDFTFVUATEVSAVADRYAEFQALGVEVISMSVDSQFVHKVWND
                     VELSKMIEAGGVPFHMGSDGGGAIGTVYGVYSPAQGVDFRGRFLIDPDGVIQAMEILT
                     PPVGRNVDETLRQVQAFQLVRKTGGKEVTPSGWTPGGKVLNPGPDLVANVWKVWNPKD
                     VYGKK"
     gene            1418..>2785
                     /locus_tag="PK442_06655"
     CDS             1418..>2785
                     /locus_tag="PK442_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO87364.1"
                     /translation="MGIASDLILVVLAGLVGGLIARRLNQPLILGYILAGIVVGPYTG
                     GITVADVDRIENLAEIGVALLLFSLGLEFSLKQILPIKRIALGGTALQALLTIALGFA
                     IGHFMGWAPLASLWFAVGIISSSTAVILKTLSSRGQLGTLSSRVMLGMSIVQDIAVIP
                     LMVLLMGLDDAVFSVLSIFVPVVKVVLFVSAMLYIGAKFIPLLLKHVARWESRELFLL
                     TVTGIGLGIGYLTYALGLSFAFGAFMAGLVLSESDYGRRALSDLIPVRDLFGLLFFVA
                     IGMLLDPAFLLDHIGVTLGLVAAVAVGRGVILSATVRLFGFRNVIPAAVFFGMIPISE
                     IAFILLQAGLDSGAVPRDVYSFALNTVILSMLLGPPATALTAPVYALLKRRRKVDAVQ
                     TVNISPDGLSGHVIVAGGGIFARHIGLVLHSMRLPYVVIEPNHALFLEAKKKGLRLLL
                     GDPGQD"
BASE COUNT          520 a          907 c          758 g          600 t
ORIGIN      
        1 gcggagagcg tccgggcggg aggctactct ctgaacagct tcttcgaggc acaagccgtg
       61 aagaaccccg ccgacgaaat cttcgacacg aaggcacagg acgcctccta tacggtcgcg
      121 ttcacgctcc tcatcttcga cggcgtcacg aacgacctct cgagactggt ccaaccgctt
      181 gagggcgccg gcattatcgt gttcgacggc ctgaaggacg ggaaatacgc cgatccgctc
      241 attctcgcgg cgccgcgccc gaagaacgcg cagacacagg gaacaagcgg atgcgctaca
      301 tccgcgggag gaatctcgcc ggccctcctg ctccttccgg gagctcttct cctgctgcgg
      361 cgaagaaaat aagcagctga aaaagcgccc cttcatcaag aaggggcgct tttttgtcgc
      421 tctattccaa acggcttact tcttgccgta gacgtccttg ggattccaca ctttccagac
      481 gttcgccacc aggtcgggac cggggttgag caccttgccg cccggcgtcc atccggaggg
      541 agtaacctct ttgccgccgg tcttacgcac aagctggaac gcctgaacct ggcgcagcgt
      601 ctcgtcgacg ttcctgccca cgggaggagt gagaatttcc atcgcctgga tcacgccgtc
      661 cgggtcgatg aggaagcgcc ctctgaaatc gacgccctga gcggggctgt acacgccgta
      721 gacggttccg atggcgccgc cgccgtcgga gcccatgtgg aagggaactc cgcccgcctc
      781 gatcatcttg gagagctcga cgtcgttcca taccttgtgg acgaactgac tgtccacgct
      841 catggagata acctccacgc ccaatgcctg aaactccgca taacggtcgg caactgccga
      901 cacttcagtc gctcagacga aggtgaagtc gcccggatag aagcagaggg aaacccactt
      961 tcccgcaaac tgcgatagct tcaccgtggt gaatccgccc ttgtaaaacg ccggcgcagt
     1021 gaaatcaggc gccttcatgc caaccgtaac acccattcga acagcctcct tcttcacctc
     1081 ttcggcgggc gcagcctcgg cgcccattcc ttcgacctcc gcagcgggaa ccgtgcatcc
     1141 taccttttcc tcgctcatga agactcctcc tttcataccg taaagcataa cattaaaaga
     1201 gacgggacaa gagagaaaaa caatcttcgt gcaaaaaaac attctttgaa accgcacgcc
     1261 tcttttattt gcttattagt atatgggaag tcatttaaaa gtcaaggatt tttattgtct
     1321 tttttacacc gcataccagt ggatgcaaga aatgctttgc actttttctc cgaggatgca
     1381 agaataagag caagaaaacc ggaaagaagg agatcgcatg ggcatcgcat cggatcttat
     1441 tctcgtcgtt ctcgcggggc tcgtcggcgg actcatcgcc agacggctga atcagccgct
     1501 catcctcggg tatatactgg cgggcatcgt cgtcggcccc tacaccggag ggatcaccgt
     1561 cgcggacgtc gaccgcatcg aaaatcttgc cgaaatcggc gtcgcgctcc ttcttttctc
     1621 cctggggctc gaattttccc tcaagcagat tctcccgatc aagcggatcg ccctcggtgg
     1681 gaccgccctc caggcgttgc tgacgatcgc gctcgggttc gccatcgggc atttcatggg
     1741 gtgggccccc ctcgcgtcgc tttggttcgc ggtagggatc atctcgtcga gcaccgcggt
     1801 catcctcaag accctctcct cccggggaca gctcggcacg ctgtcgagca gagtgatgct
     1861 gggcatgtcc atcgtgcagg acatcgcggt gattccgctg atggtgcttc tcatggggct
     1921 ggacgacgca gtcttttccg tcctctccat cttcgtcccc gtcgtcaagg tcgtgctctt
     1981 cgtctccgcg atgctctaca tcggcgcgaa gttcatcccg ctcctcctga agcatgtggc
     2041 gcggtgggag tcccgggaac tctttcttct cacggtcacc ggcatcggcc tcgggatcgg
     2101 gtacctgacc tacgcgctcg ggctctcctt cgccttcggc gccttcatgg caggccttgt
     2161 gctcagcgaa tcggactacg gccgccgcgc attgagcgac cttattcctg tccgcgacct
     2221 cttcggactg ctcttcttcg tcgccatcgg catgctgctc gaccctgcct ttctgctgga
     2281 ccacatcggc gtcaccctcg gactcgtggc tgccgtcgcc gtaggccgag gcgtcatcct
     2341 ttcggcgacg gtgaggctgt tcggcttccg caacgtcatc cccgccgcag tcttcttcgg
     2401 catgatcccg atctccgaaa tcgcgttcat cctgcttcag gcagggttgg actccggcgc
     2461 ggttccgcgg gacgtctatt ccttcgccct gaacacggtc atcctctcca tgttgctggg
     2521 tccgcccgcc actgcgctga ccgcgcccgt gtacgcgctc ctcaaaaggc ggcgaaaagt
     2581 agacgcggtc cagacggtca acatctctcc cgacgggttg tccgggcacg tgatcgttgc
     2641 cggcggaggc atcttcgccc gacacatcgg gctcgtcctc cacagcatgc ggcttcccta
     2701 cgtcgtcatc gagccgaacc acgccctctt tcttgaggcg aaaaagaagg ggttgcgact
     2761 gcttctcggc gatcccggac aggac
//
LOCUS       DAOSWW010000289         2778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27476_length_2778_cov_3.803158, whole genome shotgun sequence.
ACCESSION   DAOSWW010000289 DAOSWW010000000
VERSION     DAOSWW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27476_length_2778_cov_3.803158"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PK442_06660"
     CDS             <1..732
                     /locus_tag="PK442_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOO87365.1"
                     /translation="GEEGYPAYLGTRLASFYERAGRAIVLGGDKREASVSAIGAVSPP
                     GGDLSEPVSQNTLRVAKVFWGLDATLAYQRHFPAINWLLSYSLYTEKLDEFWDVKFDD
                     EWSPTRIEAMTLLEDEDKLREIVRLVGVDALSKEERMVLETAKSLREDFLHQNAFHEI
                     DTYASMEKQFKMLKTILAFHHISMDALKKGASMNEVFNLPVREAIARMRYLEEKDIGK
                     IDDIEGQIKNEVAGVTPVGGESDAA"
     gene            722..2140
                     /locus_tag="PK442_06665"
     CDS             722..2140
                     /locus_tag="PK442_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOO87366.1"
                     /translation="MLPKEYKTISELSGPLLLVNKTKNVRYDELVEIELASGERRRGR
                     VLEITSDKALVQVFEGTDGIDIKSTKMLFLGKVLTLPVSKDMLGRVFNGRGEPIDDGA
                     PILAEKKLDINGMPMNPFSRDYPSEFIQTGISTIDGMNPMVRGQKLPIFSGSGLPHNR
                     MAAQIARQATVISGHEDFAVVFAAMGITFEEASFFMEDFRKTGAIQRTVMFVNLADDP
                     AIERITTPRLALTAAEYLAFEQDMHVVVILTDLTNYCEALREISAARKEVPGRRGYPG
                     YLYTDLATMYERAGRLKGKKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSRTL
                     HRKGIYPPVDVMPSLSRLKDKGIGAGKTREDHADLMNQLFAGYARGKEAKELAVILGE
                     GALTEEDKAFAKFADMFEDQYVRQGEYRNRTYEETLELGWDLLTVIPVKELKRVRDAY
                     IAKYLQPRLKSEEKAQAVSGKV"
     gene            2153..2776
                     /locus_tag="PK442_06670"
     CDS             2153..2776
                     /locus_tag="PK442_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOO87367.1"
                     /translation="MARMNVNPNRMELSRLKKRLVVAKRGHKLLKDKQDALIKAFLER
                     ARQGKTLREELEAELAACYKSFLLARAQTLPAMLEQALMISGAKCDLQVGFQNVMSVL
                     VPVYRVEQGGSPLNYGLATSLGSLDVALERFAKLIPKLIELAAKEKAIRLMAVEIEKT
                     RRRVNALEHVLIPGFIETIRYITMKLDEQERSTLSRLMKIKEIVRSH"
BASE COUNT          724 a          716 c          757 g          581 t
ORIGIN      
        1 ggtgaagagg gctaccccgc ctatctcggt actcgactgg cctccttcta cgaaagagcg
       61 ggcagggcga tcgtcctggg cggagacaag agagaggcat ccgtcagtgc aataggcgca
      121 gtttcacctc cgggaggaga cctttccgaa ccggtaagtc agaatacgct tcgcgtagca
      181 aaggtattct ggggattgga tgcgacgctt gcttaccaac gccacttccc ggcgatcaac
      241 tggcttttga gctattcgtt gtacacggaa aaactcgatg aattctggga tgtcaaattc
      301 gacgatgaat ggagtcccac ccgtatcgaa gccatgacgc ttcttgagga tgaagacaaa
      361 ctgcgtgaaa ttgtgcgcct tgtcggcgtc gacgcactct ctaaagagga acggatggta
      421 ctggaaacgg caaaatcgct ccgagaggac ttccttcatc aaaacgcatt ccacgagatt
      481 gacacgtacg cttccatgga aaagcagttt aagatgctga agacaattct tgcattccat
      541 catatctcga tggatgccct caaaaagggc gcctctatga atgaagtttt caatcttcct
      601 gtcagggagg ctatcgcccg catgcgttat cttgaagaga aggacatcgg gaagatcgac
      661 gacatcgaag gccaaattaa gaacgaggtc gccggagtca ctcccgttgg aggtgaaagc
      721 gatgctgcct aaggagtata aaaccatcag cgaactttcg ggtccgcttc tgctggtaaa
      781 taaaacgaaa aacgtccgtt acgacgagtt ggtagaaata gaactcgcaa gcggtgaacg
      841 ccgccgtgga agagttctgg agatcacctc ggacaaggcg cttgtgcagg tgtttgaagg
      901 aaccgacggc atcgatatta aaagcaccaa gatgctcttt cttggcaagg tgctcactct
      961 tcccgtaagc aaggatatgc tcggcagagt ctttaatggc cgcggcgagc ctattgacga
     1021 tggagctcct attctcgcgg agaaaaagct ggatataaac ggtatgccga tgaacccgtt
     1081 ctcaagagac tatccttccg agttcattca gacgggtatc tcgacgatcg acggaatgaa
     1141 tccaatggtt cgcggccaga agctgccgat tttctccggc agcggcctgc cgcataacag
     1201 aatggccgcg cagatcgccc gtcaagccac tgtcatcagc ggacacgagg acttcgcggt
     1261 cgtcttcgcc gcgatgggaa tcacatttga ggaagcttca ttcttcatgg aggacttcag
     1321 gaagacagga gccattcaac ggacggttat gttcgtcaac ctggccgacg accccgcgat
     1381 cgagcgtatc acaacgccta gacttgcgct taccgccgcg gaataccttg cttttgagca
     1441 ggacatgcac gtcgtggtca tcttgaccga ccttactaac tactgcgaag cgttgcgcga
     1501 gatctctgcg gcgcgcaagg aagtccccgg ccgcagaggc tatcccggct acctctacac
     1561 cgaccttgcc acaatgtacg agcgcgccgg acgtctgaag gggaaaaagg gttccatcac
     1621 gcagattccc atcctcacca tgcccgagga cgacaagacg catccaatac ccgacttgac
     1681 cggatacatc accgaaggtc agatcatcct cagccgtact ctccacagaa aaggtatcta
     1741 tccccccgtg gacgttatgc cttctctgtc ccgactgaaa gataagggaa tcggcgccgg
     1801 caagacgcgg gaagatcacg cggacctcat gaaccagctc tttgccggat atgcgcgcgg
     1861 gaaagaagcg aaggaacttg cagtcatcct tggcgaaggc gctcttacag aagaggataa
     1921 ggcgttcgcg aaatttgcgg acatgtttga agaccagtac gtccggcaag gcgagtaccg
     1981 gaacaggact tatgaagaga ctctcgaact cggatgggat cttctgacag tcattcctgt
     2041 gaaagaactg aagcgcgttc gggatgcgta catcgcgaag tacctgcagc cgaggttgaa
     2101 gagcgaggaa aaggctcaag ccgtctccgg gaaagtataa gggggggtca ttatggcccg
     2161 tatgaatgtc aaccccaaca ggatggagct ctcgaggctg aagaaacgtc tcgtcgttgc
     2221 caaacgaggg cacaaactcc tgaaggataa gcaggatgct ctgatcaagg cgtttctcga
     2281 aagagcacgg cagggaaaaa cgctccgtga agagctggaa gctgagcttg ctgcatgcta
     2341 caagagtttc cttcttgcgc gtgctcaaac gctgcccgcg atgctggaac aggccctcat
     2401 gatatcggga gcgaagtgcg accttcaggt tggattccag aacgtgatga gcgtacttgt
     2461 accggtatat cgagtagaac aaggcggctc tccgctgaac tacggccttg cgacttctct
     2521 tggcagtctc gacgttgcgc ttgaacgttt tgcgaaactg attccgaaac tcatcgaact
     2581 tgctgccaaa gaaaaagcca ttcgcttgat ggctgtcgag atcgagaaaa cacgaagacg
     2641 cgttaacgct ctggaacacg ttctaattcc aggtttcatc gagacaatcc gttatatcac
     2701 gatgaagctt gacgaacagg agcgctctac actgagccgt ctgatgaaaa tcaaggaaat
     2761 agtcagatct cactgacc
//
LOCUS       DAOSWW010000290         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27540_length_2774_cov_3.895550, whole genome shotgun sequence.
ACCESSION   DAOSWW010000290 DAOSWW010000000
VERSION     DAOSWW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27540_length_2774_cov_3.895550"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PK442_06675"
     CDS             complement(<1..424)
                     /locus_tag="PK442_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOO87368.1"
                     /translation="MASRLTKTRTLAAVAVAALVGAGYFIWGAVPAKREQTEVARVAR
                     PVKTMAVRAAGGGGERIFPGKVLASQKVNLAFRVSGQIAELPAVKGTFVEPGTLLARL
                     DPRDFEVQLANAKSELGNAKARLEAMKAAADEGCRSTLD"
     gene            912..2681
                     /locus_tag="PK442_06680"
     CDS             912..2681
                     /locus_tag="PK442_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03768 family metallophosphoesterase"
                     /protein_id="HOO87369.1"
                     /translation="MNSLYPYKIMGFIFFTCMLFFFFPGRSECAVNSQSEPEGYPIAS
                     DVQTTVQRTIMPGATPTTAISLREISKYEQNGYGDWTYGPGLPHDRRLDLMPKEYNSN
                     GATQKTRLLNFFTISDIHITDKESPNQLIHLQNLHPESRYGSSLYSGVMLYTTHVLDA
                     AIQTVNALHKQNPLDFGISLGDACNTSQYNELRWYIDVLDGKVIRPSSGAHLGAETID
                     YQKPYKAAGLDTSIPWYQTMGNHDHFWMGSFPLEHRLRKDLRQSYVSDDVFATGDLLS
                     DPRKIGSRDYYMGVLDGSTPYGDVKYAGPVGDFKTQPKVAADPDRRSILRTEWMQEFF
                     NTSSLPAGHGFDMPGAKDGFACYSFVPKPNVPLKMIVLDNTQRDDSGSLDIHGHGFLD
                     AARWEWLKKELADGTAAGQLMVIASHIPISVEVTAPNSSMGWWTDPKNAVTLPDLLAE
                     LHSHPNLVLLMAGHRHLNTVKAFVSPDPAGAPENGFWQVETSSLHDFPQQFRTFEIFL
                     NSDDTISIVTTNVDPAVLEGTPAAISRRYAIAAAQILKNMEGIEHWNPTNDPTVKPMP
                     AGSYNAELVVPLSPAMREKLKNF"
BASE COUNT          604 a          831 c          770 g          569 t
ORIGIN      
        1 tgtccagcgt tgaccggcag ccttcgtccg ccgcggcctt catcgcttcc aggcgggcct
       61 ttgcgttccc cagctcgctc ttcgcgttgg cgagctgcac ctcgaagtcg cgtgggtcga
      121 gacgggccag cagcgtgccc ggctcaacaa acgtcccttt caccgcgggc agctccgcga
      181 tttgaccgga gacgcggaag gccaggttca ccttctgcga cgccagcacc ttgccgggga
      241 agattcgctc tcctccgccg cctgcggcgc gaaccgccat cgtcttcacc ggacgggcga
      301 cgcgggcgac ctcggtttgc tcgcgcttcg cggggacggc gccccaaatg aaataccccg
      361 cgccgaccag cgcagcgacc gcgaccgccg cgagagtgcg cgtttttgtg agtctgctcg
      421 ccatttttaa accacccttt cgcatcaaga aatagtttat aagaaaaaga gaagccctct
      481 tttcgctatt ataaccgaca cacaaaaaac ggggaccatg gtcttgtccc cggaaagagc
      541 gtcttcaacc tttcactccg cagcgcggtg tattttccgc gtctacttga gaagatccgc
      601 cagtgttctg atccagcccg acttcatttt ctccatcatc cagtcctcgc cgacgattgt
      661 ggcgacgtat atccctcccc ggacgtcggc gtatggtgtg cagactgaaa ttagggtttg
      721 ccgacgcacg taatcgactg tacaatgcag tcggaggaga tgcgggatgg ggagcggcgc
      781 agagtattcg ggctttgggt tcggtgtttc gacgcatcgg gcagagactg gcggagacgg
      841 gcggccgatg aacctgccga tgccttcgca acagattcac aaattctttg gtaatcaggg
      901 agagtgttgg tatgaattca ttgtatccgt acaaaataat gggtttcatc ttcttcacat
      961 gtatgctgtt tttctttttt cccgggcgtt cggaatgcgc cgtgaacagt caaagcgagc
     1021 ccgaaggata cccgatcgcc tccgacgtgc agacaacggt tcagaggacg atcatgccgg
     1081 gggcgacccc gacgactgcg atcagtctcc gggagatttc caaatacgaa cagaacggct
     1141 acggcgattg gacctacggc ccgggacttc cccacgacag acggctcgat ctcatgccga
     1201 aggagtacaa cagcaacggc gccactcaaa aaacacggct gctgaatttc tttaccatat
     1261 cggatatcca tatcaccgac aaagaatctc ccaatcagct gatccatctc caaaacctgc
     1321 atcctgaatc acggtacgga tcttctctgt attccggggt catgctgtac acaacgcacg
     1381 tgctcgacgc cgccatccag accgtcaacg cgctgcacaa gcagaaccct ctggactttg
     1441 gtatttctct tggagacgcc tgcaacacct cgcagtacaa tgaacttcgg tggtatatcg
     1501 acgttctcga cggcaaggtt atccggccca gctccggcgc gcatctggga gccgaaacca
     1561 tcgattatca gaaaccctac aaagcggcgg ggctcgacac gtccattccc tggtatcaga
     1621 caatggggaa ccacgatcat ttctggatgg gctccttccc gctggagcac agactgcgca
     1681 aggatcttcg gcaatcctac gtcagcgatg acgtcttcgc cacgggagat ctcctgagcg
     1741 acccccggaa gatcggcagc cgagactact atatgggggt gctcgacggc tcgacgccct
     1801 acggagatgt caaatacgcg ggccctgtcg gcgatttcaa gacccagccc aaagtcgccg
     1861 ccgatccgga tcgccgttcg attttgcgaa cggagtggat gcaggagttt ttcaacacgt
     1921 cgtcgcttcc cgccggtcac gggttcgaca tgcccggcgc caaggacggc tttgcatgct
     1981 acagcttcgt tccgaaaccg aatgttccgc tcaagatgat cgtgctggac aatacgcaaa
     2041 gggacgacag cggctccctc gatattcacg ggcacggctt tctggatgcg gcgcgctggg
     2101 aatggttgaa aaaggagctt gccgatggca cagcggccgg tcagctgatg gtcatcgcct
     2161 cgcacatccc gatcagcgtt gaagtgacag cgcccaattc ctcgatgggc tggtggaccg
     2221 acccgaaaaa tgccgtcaca ctgcctgatc tgctggcgga actgcacagc catccgaacc
     2281 tcgtcttgtt gatggccggg catcgtcatc tgaataccgt gaaagccttc gtgtcgccgg
     2341 atcctgcggg cgcgcccgaa aacggcttct ggcaggtgga aacgtcgtcg cttcacgatt
     2401 ttccgcagca gtttcgcact ttcgagattt tcctcaacag cgacgacacc atttccatcg
     2461 tgacaacgaa cgtcgatccc gcagttcttg aagggacgcc cgccgcgatc tcgcgcagat
     2521 atgccattgc ggcagcgcag attctcaaga acatggaagg aatcgagcat tggaatccaa
     2581 caaacgatcc cacagtgaag cccatgccgg ccgggtcgta caacgcggag ctggtggttc
     2641 ccctgagccc ggcgatgcgc gagaagctga aaaacttttg acggagaaga atggaccccg
     2701 aaaaccgggg gggagggtgc gcccttaaat aatcagagcg cgtcggaact tttcagcgga
     2761 ggtcaaagcc gtag
//
LOCUS       DAOSWW010000291         2766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27662_length_2766_cov_3.432682, whole genome shotgun sequence.
ACCESSION   DAOSWW010000291 DAOSWW010000000
VERSION     DAOSWW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27662_length_2766_cov_3.432682"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="PK442_06685"
     CDS             complement(<1..1980)
                     /locus_tag="PK442_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOO87370.1"
                     /translation="MFCMMSKNDWFPDNEWFPGDDSSSPKRPVFRLPKVNKGLLLLGG
                     AVLLVMVLFPALAEFYTDWLWYDTEGYGSVFLTRILARLPLFAAGFLLFASAIWVNLA
                     FVRRNLLALHPEFTPLLASKAAGWAIGAVALFLGVSNGAAASTEWAMVLRFFNATPFG
                     TTDPIFGNDTGFYVFALPFWTFLHTRLLNILFLCVVTAAAAYGAIILPRNRDFSMITI
                     PQSVRTHLTLLASAGAFLWGIGYLLDRYGILFSPRGVTFGAGYTDVHAELFALNVLAV
                     ISILIAAALLVSLFRKMWKLSLGMVGLLVVSSVLLRGVYPAIVQKYFVEPNEFERERR
                     FIDYNIAATLHAYNLHNVELKTITPDDAITWRDIEENRDTTDNIRLWDHGPLLRSYKQ
                     LQEIRTYYDFANVDIDRYKFPSVSGTGSSEFRQVMLAARELDIRGLQNPTWINTRLEF
                     THGYGIVMNPVNEVAGSGLPRLWVRDLPPRSDVELPITHPQIYYGEKPSSYIFVRTTV
                     QEFDYPMGDSNVRTVYEGKGGVPMGSFFRRILFALKFADMKILLSDVFTEESRVKFHQ
                     NIQERLMRVAPFLYFDRDPYLIVSEGRLVWMQDAYTAADSYPYAQPVSLGRGQNINYI
                     RNSVKATVDAYDGTMRFYAADPDDPVLKAWSGIFPE"
     gene            2261..>2766
                     /gene="dctP"
                     /locus_tag="PK442_06690"
     CDS             2261..>2766
                     /gene="dctP"
                     /locus_tag="PK442_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO87371.1"
                     /translation="MFKKSFATTAVMLLALSLFCTAAFAKVEIKLSNQLPPSHHISKG
                     LVVFSEKVAEYSGGDVTVRIFDSAQLFKDTEIVEALQDGLVEAGLVPVNKWSGMIPAA
                     DIFEMPFVFKDFESPKKFLNSGADKLLDGEFQKKGVKNLFWVDYGYIQFFNNKRPLKS
                     PEDFKGLT"
BASE COUNT          602 a          750 c          874 g          540 t
ORIGIN      
        1 ctccgggaag atgccgctcc acgccttcag caccgggtcg tcggggtctg cggcgtagaa
       61 gcgcatcgtg ccgtcgtagg cgtcgactgt ggccttcacg ctgttgcgta tgtagttgat
      121 gttctgtccc cgtccgagcg agaccggctg cgcgtagggg tagctgtccg cggcagtata
      181 ggcgtcctgc atccagacga ggcgtccttc cgagactata aggtacggat ccctgtcgaa
      241 gtagaggaac ggcgcgacgc gcatgaggcg ttcctggata ttctggtgga acttcacgcg
      301 gctctcctcg gtgaagacgt cggagagaag gatcttcata tcggcgaact tcagcgcgaa
      361 gagaatgcgg cggaagaagg aacccatcgg cacgccgccc ttcccctcgt acaccgtgcg
      421 gacgttggag tcgcccatcg ggtagtcgaa ctcctgcacg gtcgtccgga cgaagatgta
      481 ggagctgggc ttttcgccgt agtagatctg cgggtgcgtg atgggaagct ccacatcgct
      541 tctcggagga aggtcgcgca cccagagacg gggaaggccg ctcccggcaa cctcgttcac
      601 cgggttcatc acgatgccgt agccgtgggt gaattcgagg cgggtgttga tccacgtcgg
      661 gttctgcagg ccgcggatgt ccagctcacg cgccgcgagc atcacctgcc ggaattcgct
      721 cgagcccgtt cccgatacgg agggaaactt gtagcggtcg atatccacat tcgcgaagtc
      781 atagtaggtg cggatctcct ggagctgctt gtagctccgc agcagcgggc cgtgatccca
      841 caaacggata ttgtcggtgg tgtccctgtt ctcctcgatg tcgcgccacg tgatggcgtc
      901 gtcgggcgtg atcgtcttga gctccacatt gtgcaggttg tacgcgtgga gcgtcgcggc
      961 gatgttgtag tcgatgaagc ggcgttcgcg ctcgaactcg ttcggctcga cgaagtactt
     1021 ctggacgatc gccgggtaga cgcctctcag gaggacgctg gagacgacga gcagtcccac
     1081 catgccgagc gagagcttcc acatcttgcg gaagaggctt acaagcagcg ctgcggcgat
     1141 gaggatggag atgaccgcga ggacgttcag cgcgaagagc tccgcgtgga catccgtgta
     1201 ccccgcgccg aaggtgacgc cgcgcgggga gaaaagaatg ccgtagcggt cgagcaggta
     1261 gcctataccc cagagaaacg ctccggcgga tgcgagcagc gtcaggtgcg tccgcacgct
     1321 ctgcgggatg gttatcatcg agaagtcgcg gtttcgcggc aggatgatcg cgccgtaggc
     1381 ggccgcagcg gtgactacgc agaggaagag gatattcagc agccgcgtgt gaagaaacgt
     1441 ccagaagggc agggcgaaga cgtaaaagcc cgtgtcgttg ccgaagatgg gatccgtcgt
     1501 gccgaacggc gtcgcgttga agaagcgcag caccatcgcc cactccgtgg atgccgcggc
     1561 gccgttggaa acacccagaa agagcgcgac ggctccgatg gcccacccgg ccgctttcga
     1621 cgcgagaagc ggtgtgaatt cgggatggag agccagcaga ttccgccgga cgaaggccag
     1681 gttgacccag atggccgagg cgaagaggag gaagcccgca gcgaacaggg gaagcctggc
     1741 gaggatgcgg gtcagaaaga cggacccgta cccttcggtg tcgtaccaca gccagtcggt
     1801 ataaaattcc gccagggcgg ggaacagcac cattacaagg agaacggcgc ctccaagcag
     1861 caggagacct ttgttcacct tcggaagccg gaagaccgga cgcttgggag acgatgaatc
     1921 gtcgcccggg aaccactcgt tgtcgggaaa ccagtcgttt ttactcatca tacaaaacat
     1981 tgtaacggac tttctacctt tgtcaaagag tgaaaaacag cgcagcgctg gaggatatgc
     2041 catccgttga tgtcgaaaga gaaatatgcc gcgccgagtt cttttatttc aaagggtgga
     2101 attgaaggtg ggggagtaat ttgaaaatac cgctccggag cgatttatct tgacagagcg
     2161 ccgatgcgta tggtaacaag agtaatccgc gtatcaacac ggggcacgtt atttgttcag
     2221 gaaagtttca acacatttcg gtaaaggagg aaatcaaagt atgttcaaaa agtcatttgc
     2281 aacaacggca gttatgctcc tcgcgctctc cctgttctgc accgccgcgt tcgccaaggt
     2341 ggagatcaag ctttccaacc agctcccccc ctcgcatcat atctcgaagg ggctcgtcgt
     2401 tttttccgaa aaggtggcgg agtattccgg aggagacgtt acggtccgga tcttcgactc
     2461 ggcccagctc ttcaaggata cggaaatagt ggaggcgctt caggacggtc tcgttgaagc
     2521 gggtttggtg ccggtcaaca agtggtccgg catgatcccc gcggcggaca tcttcgagat
     2581 gcccttcgtc ttcaaggatt tcgagtcccc caagaaattc cttaactctg gcgcggacaa
     2641 gctccttgac ggcgagttcc agaagaaggg cgtcaaaaac ctcttctggg ttgactacgg
     2701 gtacattcag ttcttcaaca ataagcgtcc cctgaagtct ccagaggact tcaaagggct
     2761 cacaat
//
LOCUS       DAOSWW010000292         2763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27728_length_2763_cov_2.887740, whole genome shotgun sequence.
ACCESSION   DAOSWW010000292 DAOSWW010000000
VERSION     DAOSWW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27728_length_2763_cov_2.887740"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PK442_06695"
     CDS             complement(<1..399)
                     /locus_tag="PK442_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOO87372.1"
                     /translation="MSDVFDKDARSKIMRKVRSKENRSTELRLMKLFKDNGITGWRRN
                     YPVVGHPDFVFPEYKVAVFVDGCFWHGHDCRNTRPQDNEEYWQKKRERNIRRDKEITA
                     RFESRRWTVLRIWECELRKNRIEETLARIRR"
     gene            complement(406..1677)
                     /gene="dcm"
                     /locus_tag="PK442_06700"
     CDS             complement(406..1677)
                     /gene="dcm"
                     /locus_tag="PK442_06700"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOO87373.1"
                     /translation="MLQTVLPLVDPGKIGLSSAPWTMHEFFAGSGLVAYGLKGMFAPL
                     WANDICPQKASVYEANFGSEHFLLNDIKNVRGADLPFAHLSWASFPCQDLSLAGAIGG
                     IEACRSGLVWEWLRILDEMPAKPRVLLVENVSGLLSAGSGAHYRKLHSALVDRGYDCG
                     AIMLDASRFVPQSRPRVFVIAVERGRRIPEELVGSAPCWLHSKAAAELGKTLPGWIWW
                     RSEEPPKRHTRLEDILEPDVPFDKDDVLRLIPERHREKLDEHATVYATGYRRTRQGRQ
                     QLELRFDGIAGCLRTPEGGSSKQFIVLKKDGEVHARLLTVRETARLMGAPDSFRLPGS
                     ANDGYKAMGDAVALPVARFIGEQFLLKIAEAAYDDRQASNADLGRRVVYEHSLFDETN
                     RSAAIAKIVDRYNDIVAAAETDPSLRIEFER"
     gene            complement(1872..2516)
                     /locus_tag="PK442_06705"
     CDS             complement(1872..2516)
                     /locus_tag="PK442_06705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOO87374.1"
                     /translation="MNATIVIRNEKEADYKRVEEITRKSFWNLYVPGCTEHYLVHVLR
                     THEDFLPELDFVIEVDGRIIGNIMYTKTLLVDESGEEKNILTFGPVCIVPEYQRKGYG
                     KRLMERSFERAVALGYDVIVIFGNPNNYVSRGFKSCKKFNVCLENGTYPAPMMVKELQ
                     PGALDGRKWVYHQSSVFEIDEHEAARFDESLEPWEKKYEPSQEEFYIHCHSVIQ"
BASE COUNT          522 a          832 c          749 g          660 t
ORIGIN      
        1 ccgccgtatt cgcgccagcg tttcctcaat acgatttttc ctcaactcgc actcccaaat
       61 tcgcaggacc gtccaccttc ggctttcgaa acgggcggtt atctccttgt cgcggcgtat
      121 gttgcgctcc cgcttctttt gccagtactc ctcgttgtct tgagggcgcg tattccggca
      181 gtcgtgcccg tgccagaagc agccgtccac aaagacggcc actttgtatt cgggaaagac
      241 gaaatcggga tgtcctacaa ccgggtagtt gcgccgccac cctgtaattc cgttgtcttt
      301 gaatagtttc atcaaacgca attccgtcga ccggttttct tttgatcgca ccttccgcat
      361 tatcttcgag cgagcgtctt tgtcgaatac gtcgctcacg gcgcgttacc tctcgaactc
      421 gatgcgaagg cttgggtcgg tctcggcagc ggcgacgatg tcgttgtacc tgtccacgat
      481 cttcgcgatc gccgcgctgc ggttcgtctc gtcgaaaaga ctgtgctcgt agacgacgcg
      541 tcttcccaaa tccgcgttgg acgcctgtcg atcatcgtac gccgcctccg ctatcttcag
      601 caaaaactgt tcgcctatga agcgcgcgac gggaagcgct acggcgtcgc ccatggcctt
      661 gtacccgtcg ttggcgctgc cgggaagcct gaaactgtcg ggcgcgccca tcagacgggc
      721 tgtttcgcgg acggtgagca gtcgtgcatg aacctcgccg tcctttttga gcactataaa
      781 ctgcttgctg cttccgccct ccggcgtcct caagcagccg gcgatgccgt cgaagcgcag
      841 ttcaagctgc tgacgtccct ggcgggtcct ccggtatccg gtcgcgtaga ccgtggcatg
      901 ttcgtcgagc ttttccctgt gtctctccgg aatcagccgg aggacgtcgt ctttgtcgaa
      961 cggaacgtcc ggttcgagga tgtcctccag tcgcgtatgg cgcttcggag gctcttccga
     1021 acgccaccat atccagccgg gcagcgtctt ccccagctcc gcggccgcct tgctgtgcag
     1081 ccagcacgga gcggaaccga caagttcctc cggaatgcgg cgtccgcgct cgacggcgat
     1141 gacgaacacc ctggggcgcg actgcggcac gaaacgggac gcgtccagca tgatcgcgcc
     1201 gcagtcatac cctctgtcga cgagcgccga atgcagtttt ctgtagtgtg cgccgcttcc
     1261 tgctgaaaga aggccggaga cgttttcgac cagcagaacg cgcggcttcg ctggcatctc
     1321 gtcgaggata cggagccact cccagaccag cccgctgcgg caggcttcga taccgcctat
     1381 agcgcctgcc agcgacaggt cctgacaggg gaagctggcc caggacaggt gcgcgaacgg
     1441 caggtcggcg ccgcggacgt tcttgatgtc gttcaggaga aaatgctccg aaccgaaatt
     1501 cgcctcgtag acgctcgcct tctgcgggca gatatcgttc gcccaaaggg gagcgaacat
     1561 gcccttcaga ccgtaggcga ccagaccgct tccggcgaag aattcgtgca tcgtccacgg
     1621 cgcggacgaa aggccgatct ttccggggtc cacaagcggc aagacggttt gcagcatacg
     1681 cgttcactcc ttttcggcgt ttgtctatgt tccttgcggg attacgtatc gcccggccgg
     1741 cggaggaaga cgccgcagcg ggggatactc ctgatcatac taccgttatc cgcccgcttt
     1801 atcaacatgt tgaaacgcct gacgcgactc ccggaaccaa agcgacagcg caaaatgccg
     1861 cgaaagtgca gtcactgtat aacggaatgg cagtgaatgt aaaattcttc ctggctgggt
     1921 tcgtactttt tctcccatgg ttccaagctt tcgtcgaagc gtgcggcttc gtgttcgtct
     1981 atctcgaaca cggagctttg atggtatacc cactttcttc cgtccagggc gcccggctgc
     2041 agctccttta ccatcatcgg cgcggggtat gtcccgtttt caaggcagac gttgaacttt
     2101 ttgcagcttt tgaaaccgcg gctgacgtaa ttgttggggt tgccgaaaat gacgatcacg
     2161 tcgtacccga gcgcaaccgc gcgttcaaag gagcgctcca taagtctttt tccgtaccct
     2221 tttctctggt attcgggcac gatgcacacc gggccgaagg taaggatgtt tttttcttct
     2281 ccggactcgt cgacgagcag tgttttcgta tacatgatat tcccgatgat tcgaccgtcg
     2341 acttcgatta caaaatccag ctccggcaga aagtcttcgt gggtccgtag gacatggact
     2401 aaatagtgct ccgtacaccc cggaacgtat aaattccaga aagatttcct ggtgatctct
     2461 tcgactctct tataatcagc ctctttttcg tttctgatga cgatcgttgc gttcatagaa
     2521 agcctccact atttttatgc ccccgaatca gtagcgcaag gatattcgat gaacatcctt
     2581 gcctttccgg gagaagttcg cttgcgggac gctggaaggc ctcggcaagc gacaattttt
     2641 tattatactc ccgttctgct catgctacgg gcggagcgtt ttctctccct gcctgtgtaa
     2701 aaaaacgatc tccgatctct ccggaacaat cgagctcttc ggcggattca gctccacctt
     2761 ggg
//
LOCUS       DAOSWW010000293         2761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27751_length_2761_cov_3.199926, whole genome shotgun sequence.
ACCESSION   DAOSWW010000293 DAOSWW010000000
VERSION     DAOSWW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27751_length_2761_cov_3.199926"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PK442_06710"
     CDS             <1..768
                     /locus_tag="PK442_06710"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HOO87375.1"
                     /translation="IGGLMIAGGAYLSFIQKKKSEGLVTELKFMKAVSLGELKESWQS
                     LSDEGMADGFRDMVETNGKAGTDGEMRAPYSNTPCAYFEATVTREFEKEERSTDKDGK
                     VQSTRTRSSEVVSSRKSPSPLYIADGDIRVGIDMDGATLHLKDGEDRFEPYESNKTYT
                     FFGVQFSAPSGVRTLGFRYKEKLIPLGHPLYVVGEARHSAGALRIGKPSEKGKPFIVS
                     VKSEDEVVSGMQGSVKFVFYLGIALMIGGAAVAVFFR"
     gene            800..2023
                     /locus_tag="PK442_06715"
     CDS             800..2023
                     /locus_tag="PK442_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87376.1"
                     /translation="MKKGIVARVLVAVLLVLAVVSTGFAADPKYSKSSTFGPMITGGV
                     TDEFLQMVREDPDIKKGKPLRLGKDITDDDLKKVATLADVLTGVLIERNDTITDLSPL
                     SSLSGLTYMKLDGLKGVKTLAPVGALVKMKQLEIRQVEYPDLDFLTGMNDLESLIFFM
                     QPKQIEDISPLKGKAKLKTLRLYGGAGQDGISDISVLEELTELEDLSLYMTKVEDLSP
                     ISGLTKLKRLDLYGIPATDMSPVENLTELRYIWIYATKFEDYSPLANLTKLEEFRAGI
                     SKLDTLEYLESMPKLKIVSMLREDIQDFSPLAKCPNLTEVNLEAVKGVIDLSVFAEHK
                     KLKNLFVRNAEVKNAEAIVALPRLEWLILIDTKGIADVSIFKDLPKLNRVSVKEGQFP
                     AEQVEALGKKASVQK"
     gene            2486..>2761
                     /locus_tag="PK442_06720"
     CDS             2486..>2761
                     /locus_tag="PK442_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87377.1"
                     /translation="MKKRVSAVVWALLLAVLVVSPVSANTLNALPAWPDSTLYGVANI
                     EEPKEFINALVDSKLFKMAAAMEQDMQMAADWLKQFPVQSASVVVGLT"
BASE COUNT          643 a          718 c          835 g          565 t
ORIGIN      
        1 atcggcggtc tgatgatcgc agggggcgcg tatctctcgt tcattcagaa gaagaagtct
       61 gaagggcttg tgaccgagct gaagtttatg aaggccgttt cgctcggcga gctgaaggag
      121 tcgtggcagt cgctctccga cgagggaatg gccgacggat tccgagacat ggtcgaaacc
      181 aacggcaagg ccgggactga cggagagatg cgtgcgccgt acagcaacac tccgtgcgcc
      241 tactttgaag cgacggtcac tcgtgaattc gagaaggaag agcgttcgac cgacaaggac
      301 ggaaaggtgc agagcacgcg taccagaagt tccgaggtcg tctcgtccag aaagagccct
      361 tcgccgctct acattgctga cggagacatc cgcgtcggca tcgacatgga cggagcgacg
      421 cttcatctca aggacggaga ggaccggttc gaaccgtacg aaagcaacaa gacgtatacg
      481 ttcttcgggg tgcagttctc cgctccctcg ggagttcgga cgctcggctt ccgctacaag
      541 gagaagctca ttcctcttgg acacccactg tacgtcgtcg gggaggcgcg ccattctgcg
      601 ggagcgctgc gcatcggcaa gccttctgaa aaggggaagc ccttcatcgt ctcggtgaag
      661 tcggaggacg aggttgtctc cgggatgcag gggtctgtga agttcgtgtt ctatctgggg
      721 atcgcgctga tgatcggcgg ggcggctgtg gccgtcttct tccggtagcg cgaaacgctc
      781 tgcgaggagg ggaaattcga tgaaaaaggg aatagtggca cgcgttctgg ttgccgtgtt
      841 gctggtactg gcagtggttt cgacggggtt tgccgcagac cccaagtact cgaagagctc
      901 gacgttcggt cccatgatta ccggcggtgt gacggacgag ttcctccaga tggttcggga
      961 ggacccggac attaaaaagg ggaagccgct ccgtctcggg aaggacataa ccgacgacga
     1021 tctgaagaaa gtcgcgacgc tcgccgatgt gctgacagga gttctgatcg agaggaacga
     1081 cacgatcacc gacctttcgc cgctctcctc gctctccggg ctgacctaca tgaagctcga
     1141 cggcctcaag ggtgtcaaga cgctcgcgcc ggtcggagcg ctcgtgaaga tgaaacagct
     1201 tgaaatacgc caggtcgagt accctgatct ggatttcctt accgggatga acgatctcga
     1261 atcgctcata ttctttatgc agcccaagca aatcgaggac atctccccgc tcaaggggaa
     1321 ggcaaagctg aagacgctac gcctctacgg aggggccggg caggatggca tatcggacat
     1381 ctccgtgctg gaggagctga cggagcttga agatctcagc ctctacatga ccaaggtgga
     1441 ggacctctcc cctatctccg gcctgacgaa gctgaagcgg ttggatctct acggcattcc
     1501 cgcaaccgac atgagccctg tcgaaaacct cacggaactc cggtacatct ggatctatgc
     1561 gacgaagttc gaagactact cgccgctcgc caacctgacg aagctcgagg agttccgcgc
     1621 cggaatttca aagctcgata ctcttgaata cctggagagc atgcccaagc tgaagatcgt
     1681 ctccatgctc cgcgaggaca ttcaggactt ttcgccgctg gcgaagtgcc cgaacctgac
     1741 cgaggtcaac ctggaagctg tgaaaggagt tatcgacctg agcgttttcg cggaacacaa
     1801 gaagctgaag aatttgttcg tccgcaatgc agaggtcaag aacgccgaag ccatcgtcgc
     1861 cctgccgagg ctggagtggc tgatcctcat cgacacgaag ggtattgccg acgtctctat
     1921 tttcaaggat cttcccaaac ttaacagggt ctccgtgaag gaggggcagt tcccggccga
     1981 acaggtcgag gctttgggta aaaaagcgtc ggtgcagaag tgatgccgcg gaaggaggcc
     2041 gctaccgcct ccttcgacgg ccttcgaggg ggattgatcc gcatgaagag gtctcttgag
     2101 gaggctcccg aacagagtgt accgcaaggt cggcgcagta aaaaactctc ccgtgccgaa
     2161 cttcgtatcc ggttgcggct cgaacccttc aacctcacaa aaaaagagac ggaaatagcc
     2221 gtgtttcttg tggagggatt ggagagaaac gagatactgt ttcgatgtga tattaccaat
     2281 aatacattaa aaacgcatat tcgtcggatc tatcggaaac ttgaggtgag aaaaaggagc
     2341 gaaatcgctt caagactcgc gctgacgtga caaaacgata ttggaggagt actcttatat
     2401 gcaccccggt atcatccggg gaaaactgtc ggagcggcga gccgtgttca acatggccgt
     2461 tcgttcccat cgaggagggg ttcctatgaa aaagagagtt tcggcagtag tatgggcgtt
     2521 gctattagcg gttcttgttg tatctccggt gagcgcgaac accttaaacg cacttccggc
     2581 atggcccgac agcacgctct acggagtagc gaatatcgag gagccgaagg aattcatcaa
     2641 cgctctggtc gattccaagc tcttcaagat ggcggctgct atggagcagg atatgcagat
     2701 ggcggcggat tggctgaaac agtttcctgt gcagtccgcg tccgttgttg tcggccttac
     2761 c
//
LOCUS       DAOSWW010000294         2756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_27822_length_2756_cov_3.312477, whole genome shotgun sequence.
ACCESSION   DAOSWW010000294 DAOSWW010000000
VERSION     DAOSWW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27822_length_2756_cov_3.312477"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PK442_06725"
     CDS             <1..645
                     /locus_tag="PK442_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOO87378.1"
                     /translation="ILYDGGRIMDTSTGRAVSETSMDPALALEIVQAGWEHPVELQII
                     GDERAVCRSADAVTRGFFAEAGVPVDSSLESPSVPQEVFRVIFHGIPGSIRSLERDLK
                     SRFAGRAEIVQAGEGFLDVLPLGVSKGAALRGWLATLPELPEIVVAAGDHHNDMEMLK
                     EADIAVVPEDAADEVLALSDVIMPSADKDGFVFLADWLLKGDIHRDENASPVVL"
     gene            692..1189
                     /locus_tag="PK442_06730"
     CDS             692..1189
                     /locus_tag="PK442_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOO87379.1"
                     /translation="MPEIKKWELVQLDFPSDCNIILGQSHFIKTVEDLFEALATSSPA
                     LQFGIAFCEASGDCLIRFDGNNEELVKIAVDNMNSLGAGHSFLIVLKNGYPINVLNRI
                     KECQEVCRVFAATANPIQVIICESEQGRGVAGVIDGFSPKGVETESDIEGRKTLLRRI
                     IGYKR"
     gene            1288..2478
                     /locus_tag="PK442_06735"
     CDS             1288..2478
                     /locus_tag="PK442_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87380.1"
                     /translation="MSLLRIPVLLVLFLLFVPALPAISAPSDLIVGVEDGWIWQVALL
                     PPDDGWESDAGRSALGAVRYAERKVQESPDGVSGRDIRFHQEESLDEASAAARVEDWR
                     KRGISAVLSFAGGREIAFLRPHLGEAGPVFLSAFGETANIYNGEGVPDTMVFALDLFR
                     DFRIAAFTAYAAELFPSGSVLAVLGDKLDPALERYSRNLGDMFFDRGFLVAHFWIPGG
                     GMDSFKMIESEAIASGAKALVFWAGSMVVRDVWRSTRRKSNAFEIWYGGVPQPLLLSF
                     NGVVTADQDYPVRTDSALKSLGRDIWRRHNVTVKDPAIAGRAYAACQWLFDAFKRAGS
                     PVPAALAGMLPLAEGLVFGSQALSINPATHRPAERAVAFMIVEGREFHPVKTLLVRGP
                     DYLP"
     gene            2529..>2756
                     /locus_tag="PK442_06740"
     CDS             2529..>2756
                     /locus_tag="PK442_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOO87381.1"
                     /translation="MKLRDIASQIRAEVLSGEELLDDIDIECAYGADLMSDVLAFARP
                     GSLLLTGLTNIQIVRTAQMLDLPAVVFVRGKK"
BASE COUNT          528 a          787 c          867 g          574 t
ORIGIN      
        1 atcctctacg acggggggcg gatcatggac acttccaccg ggagagctgt ttccgagacc
       61 tccatggatc cggcgctggc gctggagata gtgcaggcgg ggtgggagca ccccgtggag
      121 cttcagatca tcggcgacga acgtgccgtc tgccgaagcg ccgacgcggt gacccggggc
      181 tttttcgcag aggcgggcgt tcccgtcgat tcctcgcttg aatcgccctc cgtccctcag
      241 gaggtattca gggtcatctt tcacggcatt cccgggagca taaggtcgct cgagcgggat
      301 ctgaagagcc gcttcgccgg aagagcggag atagtgcagg ctggggaagg gtttctcgat
      361 gttcttcctc tcggcgtttc aaagggagcg gcccttcgcg gatggctcgc gacgctgccg
      421 gagcttccgg agatcgtggt cgcggccgga gatcaccata acgacatgga gatgctgaaa
      481 gaggccgata tcgccgtcgt tccggaggac gcggcggacg aggtgcttgc gctttcggat
      541 gtgatcatgc cgtcggcgga caaggacggc tttgtcttcc tcgcggactg gctcctcaag
      601 ggagatattc atcgtgacga aaacgcatct cctgttgtat tatagatagg ccgctacaat
      661 tacccctgaa aagaagaggg agggacaatt catgcctgaa attaagaagt gggagcttgt
      721 gcagcttgat tttccgagcg actgcaacat tattctcgga cagagccatt tcatcaagac
      781 cgtcgaggac ctgttcgaag cccttgcgac ctcctctccg gcgctgcagt tcggtatcgc
      841 tttctgcgaa gcctccggag actgcctcat acggttcgac ggcaacaatg aagagctcgt
      901 caaaatagcc gtggacaaca tgaactccct gggagcggga cattcctttc tcatcgtcct
      961 caagaacggg tacccgatca atgtcctcaa caggatcaag gagtgtcagg aagtctgccg
     1021 cgtctttgcg gccacggcca atcccattca ggtcatcatc tgtgaaagcg aacaggggag
     1081 aggcgtggcg ggcgtcatcg acggcttctc tcccaagggc gtggagaccg aaagcgacat
     1141 cgaggggaga aaaacactgc ttcgccggat aatcggctac aaacggtaat ggtcgcgcat
     1201 caatctcgga gagcgtgcgc ctcggacggt cggaagggcc tgacgaagag aagacctgta
     1261 tgcgggaccg gtcgttctcc tctctccgtg agccttctgc gcatccctgt tcttttggtg
     1321 ctctttttgc tgttcgtacc tgcgcttccc gccatttccg cacccagcga cctgatcgtc
     1381 ggcgtggagg acgggtggat atggcaggta gcgcttctcc cgccggatga cgggtgggag
     1441 agcgacgcgg ggcgttccgc gctcggagcc gtacggtacg ccgaacggaa ggtgcaggag
     1501 agtccggacg gagtgagcgg ccgcgacatt cgcttccacc aggaggaatc gcttgacgaa
     1561 gcttccgctg cggcgcgggt tgaggactgg cggaagcgcg gaatctccgc agtgctttcc
     1621 tttgccggag ggagagaaat cgcctttctc cggccgcatc tcggagaagc gggacccgtc
     1681 tttctctctg ctttcggaga aaccgcgaac atttacaacg gcgaaggcgt ccccgataca
     1741 atggtcttcg cgctggatct gttccgcgac ttcagaatcg ccgcgttcac cgcgtatgcg
     1801 gcggagctct tcccttccgg gagcgttctt gcggtcctcg gcgacaagct tgaccccgcg
     1861 cttgagcggt attcccggaa cctcggcgat atgtttttcg ataggggctt ccttgtggcg
     1921 cacttctgga ttcccggcgg aggaatggat tccttcaaga tgatagaatc ggaagccatt
     1981 gcttcaggcg ctaaagccct cgtcttctgg gcggggtcga tggtggtcag ggacgtctgg
     2041 cgaagcaccc gtcgcaagag caacgctttc gagatctggt acggcggcgt tccgcaaccg
     2101 cttcttctct ccttcaacgg cgttgtgacg gccgaccagg actaccccgt ccgcactgat
     2161 tcggctctga agtcgctggg cagggacatc tggagacggc ataacgtcac cgtaaaagac
     2221 cccgcaatcg cggggcgcgc gtatgcggcc tgccaatggc ttttcgacgc cttcaagcgg
     2281 gcgggctctc ccgtcccggc cgccctggcg ggtatgctgc ccttggccga ggggcttgtc
     2341 ttcggttctc aggcgctttc aatcaatcct gcaacacacc gtcccgccga gagggccgtg
     2401 gcgttcatga tcgtggaggg gagagagttt caccccgtca aaacgctcct tgtcagagga
     2461 ccggattacc tgccgtaaag gcccctgtcc cggggaagtg ttcaagataa tctagaggag
     2521 gaagtagaat gaaactgcgt gatattgcgt ctcagatacg cgcggaggtg ctcagcggcg
     2581 aagagctgct cgacgatatc gacatagagt gcgcatacgg ggccgacctg atgagcgacg
     2641 tgcttgcgtt cgcccgcccc ggatcgctgc tcctgaccgg attgaccaac atccagatag
     2701 tgcggaccgc gcagatgctc gaccttccgg ccgttgtctt cgtccggggg aaaaag
//
LOCUS       DAOSWW010000295         2739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28071_length_2739_cov_6.323398, whole genome shotgun sequence.
ACCESSION   DAOSWW010000295 DAOSWW010000000
VERSION     DAOSWW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28071_length_2739_cov_6.323398"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1211
                     /gene="tuf"
                     /locus_tag="PK442_06745"
     CDS             9..1211
                     /gene="tuf"
                     /locus_tag="PK442_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008426562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOO87382.1"
                     /translation="MAKEKFERSKPHLNIGTIGHIDHGKTTLTAAITKCLATSNYANF
                     TAYDMIDKAPEERERGITINISHVEYQTDHRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHVLLARQVNVPALVVFMNKTDQVDDPELLDLVEMEVRELLSK
                     YEFPGDDIPVVRGSALKVMEEGNGTREDPISKCVWELMDACDSYIPEPIRDVDRPFLM
                     PIEDVFTITGRGTVVTGRVERGIIKGGEEVAIIGMKRDITKTVATSLEMFRKILDSAM
                     AGDNVGVLLRGVDKEGVERGQVLAKPNSIKPHTKFKAEVYVLKKEEGGRHTPFFAGYK
                     PQFYFRTTDVTGSIKLPEGVEMVIPGDNATFEVELIVPIAMDEGLRFAVREGGHTVGA
                     GVVTEILE"
     gene            1237..1386
                     /gene="rpmG"
                     /locus_tag="PK442_06750"
     CDS             1237..1386
                     /gene="rpmG"
                     /locus_tag="PK442_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOO87383.1"
                     /translation="MADIVGMACTECKRRNYSTTVNKKKQAKKLELNKFCKWCGKHTL
                     HKEGK"
     gene            1408..1483
                     /locus_tag="PK442_06755"
     tRNA            1408..1483
                     /locus_tag="PK442_06755"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1441..1443,aa:Trp,seq:cca)
     gene            1792..2334
                     /gene="nusG"
                     /locus_tag="PK442_06760"
     CDS             1792..2334
                     /gene="nusG"
                     /locus_tag="PK442_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOO87384.1"
                     /translation="MDSIEARRWYIVQTYAGYENRVKANLEQRIATMGMEDSIFGVLV
                     PVEERVSIKEGKSKKVTRKLFPSYVLVEMRLNDQSWYVVRHTPGVTGFVGSGNHPIPL
                     TEKEVKEIMGKVGKDQAKPKIEINFRPGDVVKVKSGPFEGHVGPVVEIFPEKGKVKFS
                     VTVFGRETSVETDYTELEKL"
     gene            2443..>2739
                     /gene="rplK"
                     /locus_tag="PK442_06765"
     CDS             2443..>2739
                     /gene="rplK"
                     /locus_tag="PK442_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOO87385.1"
                     /translation="MAKKVIAEIKLQLPAGKATPAPPVGPALGQHGVNIMEFVKQFNA
                     KTADQAGLVIPAVITVYADRSFTFVLKTPPASVLLKKAAGLESGSGEPNKKKVAT"
BASE COUNT          690 a          651 c          859 g          539 t
ORIGIN      
        1 tttctacaat ggcgaaggag aaatttgaga ggtcgaagcc gcatttgaac ataggtacga
       61 tcggacatat cgaccacggg aagacgacgc tgacggcggc gatcacgaag tgtcttgcga
      121 cgtcgaacta cgcaaacttc acagcgtacg acatgatcga caaggcgccg gaagagagag
      181 agcgcggcat cacgatcaac atatcgcacg ttgagtacca gacggaccac cgtcactacg
      241 cgcacatcga ctgcccgggg cacgcggact acatcaagaa catgatcacg ggagcggcgc
      301 agatggacgg agcgatcctc gtggtgagcg cggccgacgg cccgatgccg cagacgcgcg
      361 agcacgtgct gcttgcccgt caggtgaacg tgcctgcgct ggtggtgttc atgaacaaga
      421 ccgaccaggt ggacgatccc gagcttctcg accttgtcga gatggaagtg cgggagcttc
      481 tgagcaagta cgagttcccc ggcgacgaca tccctgtggt tcgcggatcg gcgctgaagg
      541 ttatggaaga ggggaacgga acgcgcgagg atccgatcag caagtgtgtc tgggagttga
      601 tggacgcatg cgacagctac attccggagc cgattcgcga cgtagaccgt ccgttcctga
      661 tgccgatcga agacgtgttc acgatcacgg gccgcggcac ggtggtcacg ggcagagttg
      721 agcgcggaat catcaagggc ggcgaagaag tagcgatcat cggaatgaag agagacatca
      781 cgaagacggt ggcgacctcg cttgaaatgt tccggaagat ccttgacagc gcgatggccg
      841 gagacaacgt cggcgtcttg ctgagaggcg tggacaagga aggcgtggag cgcgggcagg
      901 tattggcgaa gccgaactcg atcaagccgc acacgaagtt caaggcggaa gtctacgttt
      961 tgaagaagga agagggcggg cggcacacgc cgttcttcgc gggatacaag ccgcagttct
     1021 atttccgtac gacggacgtg acgggaagca tcaagctgcc cgagggagtg gagatggtca
     1081 ttcccggaga caacgcgacg ttcgaggtcg agctgatcgt gccgatagcg atggacgaag
     1141 ggcttcgttt cgcagtgcgc gaaggcggcc acacggtcgg cgccggcgtc gtcaccgaaa
     1201 ttctagagta gttcaatatc gaggaggcac gtgtaaatgg ctgatatcgt aggaatggcg
     1261 tgcaccgagt gcaaacggcg taattacagc acgacggtga acaagaagaa acaggcgaag
     1321 aagctcgagc tcaataagtt ttgtaagtgg tgtggaaaac acaccttgca taaagaaggg
     1381 aagtagtgta gaatactctc tgcttgcagg tccgtagctc aattggcaga gcactggtct
     1441 ccaaaaccgg gggttgcagg ttcgagtcct gccgggcctg ccattttttt attgtcaaaa
     1501 acaagatggg gggcagaggt tgtgggaaat atcctcacct tcgttcggga agcccgggcc
     1561 gagttgaaga aagtaacgtg gccggggaaa aagcaagtct ggtattcaac aatagtcgtt
     1621 atcgctttta cgcttttggt ttcggcctat ctcgggatag ttgatatggt acttacagga
     1681 gtcttttcca ggctctttag ttaaaagccc gctgccggtc caggcagttg ataccgggat
     1741 catttcagag atcggcaggg aggtgagggg gccgcaagac ccccttaaag tatggacagt
     1801 attgaagcgc gccgctggta tatagtacaa acgtatgcag ggtacgaaaa tcgcgtcaag
     1861 gcaaatctcg aacagagaat tgccacgatg ggtatggagg acagtatttt cggcgtcctc
     1921 gtccctgttg aggagcgtgt ctccataaaa gagggaaaga gcaaaaaggt gactcgcaag
     1981 ctttttccca gttatgttct tgtcgagatg cgtcttaacg accagtcgtg gtatgtcgtt
     2041 cggcataccc ccggcgtgac tggctttgtc ggctccggaa accatccgat tcctctcact
     2101 gaaaaagaag tgaaagagat catggggaag gtcggtaaag accaggcgaa acccaaaatc
     2161 gagattaatt tccggcctgg cgatgtcgtc aaggtaaaat ccggaccctt tgaaggtcat
     2221 gtggggccgg ttgtcgagat tttcccggaa aagggaaaag tgaagttctc tgtgacggtc
     2281 ttcggccggg agacatctgt agaaactgac tatacggagc ttgagaagct ctagatatat
     2341 atcagcgcga aggcccgcat tccagggagg caagagaact cttggctcct gtttttccct
     2401 gtgtgcggaa gtgctttcag taacgagagg agactttgag gaatggcgaa gaaagtaata
     2461 gcagagatca agttgcagtt gcccgcaggc aaggctacgc ctgcgccccc cgtgggacct
     2521 gctcttggtc agcacggagt gaatatcatg gagttcgtga agcagttcaa cgcgaaaaca
     2581 gcagatcagg ccgggcttgt aattcccgcc gtgatcacgg tgtacgcgga cagaagcttc
     2641 accttcgtgc tcaaaacacc gcccgcgagc gtgctgctca agaaggcggc cggattggaa
     2701 tccggctccg gcgaaccgaa caagaagaag gtagccacc
//
LOCUS       DAOSWW010000296         2737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28110_length_2737_cov_3.462342, whole genome shotgun sequence.
ACCESSION   DAOSWW010000296 DAOSWW010000000
VERSION     DAOSWW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28110_length_2737_cov_3.462342"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PK442_06770"
     CDS             <1..337
                     /locus_tag="PK442_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOO87386.1"
                     /translation="DGGTLIPWAVIPVAAICGATLEVVRAGLTRGVRHTSNALGYLKA
                     VLPGYADEEFYVDRPAVADGNLITAGGLDRIEFAYEIVKRLEIFDEAEAGELYEMFKN
                     GVVPARYMA"
     gene            complement(433..1161)
                     /locus_tag="PK442_06775"
     CDS             complement(433..1161)
                     /locus_tag="PK442_06775"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOO87387.1"
                     /translation="MFNILETREKLLKKFVEQGNEPRILLKGAGYGIRVILQDDAAED
                     ELLEEARASVEELARIAGDTGLVIDCKMRKLTPDFLSRLLCDFVWAGGFRVLSWTSDD
                     TETLELFRNTGFTTGEPVSGRPDRERVESGSLILTQSLRSGQMVEHDGDVMLLGHMNE
                     GAEIRASGNIFVHGRLKGVVHAGTRGGEHGVWAERFEATQVRIGGKICSSLGRESAWW
                     GKAVLIALENGQLVARELKLDFAS"
     gene            1403..2308
                     /locus_tag="PK442_06780"
     CDS             1403..2308
                     /locus_tag="PK442_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOO87388.1"
                     /translation="MEVQNNEWQVVVFFLGGQAFAISVDKTREILRWPGSRPIPESHP
                     AMIGITSVRGEVMPLLDLRAYLGITPKVPLESSKVIIAEFNESKLGFVVDGVERIYGI
                     SAAELDSTLTSAVLGKSILYVIKRNESNVLIPDYEAIIQAAAPSLDRSLSIDLKKVAE
                     LAAPLGDLDRYRILVAEDSPLIRTQISDVLTQGGFHNVTLVADGKEAWERLAMKNERY
                     DLLVTDVEMPRLDGVALTKQVKEHPELKRMPVVVFSSIMVQDVRLKISGTGADAHVTK
                     PELPRMVEKVCRLLLDSKEKAAATK"
     gene            2369..>2737
                     /locus_tag="PK442_06785"
     CDS             2369..>2737
                     /locus_tag="PK442_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOO87389.1"
                     /translation="MKVLVSACTIGCNCKYNGKNNRNEAVVEYLRDKEVISICPEALG
                     NMGTPRPCAEIVEGVAMDEHGKNVDAEFRNAVELALEQIKDEAIDLVILQSRSPTCGV
                     NRIYDGTFSGKLIPGQGLFAQ"
BASE COUNT          559 a          820 c          836 g          522 t
ORIGIN      
        1 ggacggcggc acgctcatcc cgtgggcggt cattcctgtc gccgcgattt gcggggcgac
       61 gctggaggtc gttcgggccg ggctgacgcg cggcgtccgg catacgagca acgccctcgg
      121 ctacctcaag gcagtgctgc ccggatacgc cgacgaggag ttctacgtgg accggccggc
      181 ggtcgccgac ggaaatctga taacagcggg aggactcgac aggatcgagt tcgcgtacga
      241 aatcgtcaag cggctggaaa tcttcgacga agccgaggcg ggcgagctgt acgagatgtt
      301 caaaaacggc gtcgtcccgg caaggtatat ggcgtaacat gcgaaaggga gagcgcggcg
      361 gccgttgcgc tctccccttc atggagttcc gcgacgccgc agagcgcctt caggggcgcg
      421 ggcgtctttt ttttatgacg cgaagtcgag tttcagctcc cgtgcgacca gctgcccgtt
      481 ctccagcgcg atcagcactg cctttcccca ccaggcgctc tccctgccga gggaggaaca
      541 gatcttcccc ccgatgcgca cctgcgtcgc ctcgaagcgc tccgcccaca cgccgtgctc
      601 gccgccgcgc gttcccgcgt ggactacgcc cttgagcctg ccgtggacga agatgttccc
      661 ggacgcacgg atttccgcgc cctcgttcat gtgccccaga agcatgacgt cgccgtcgtg
      721 ctccaccatc tgccccgagc gaagcgattg cgtcaggatg agcgagcccg attcgacgcg
      781 ctctctgtcc gggcgtccgg agaccggctc tccggtggtg aaccccgtat tgcgaaaaag
      841 ctccagcgtc tccgtatcgt ccgacgtcca tgaaagaacg cggaaacctc cggcccatac
      901 gaagtcgcag agcagtcggg aaaggaaatc cggcgtgagt ttgcgcatct tgcagtcgat
      961 caccaggccg gtgtcgccgg cgatgcgggc cagttcttcg acggaggcgc gcgcctcctc
     1021 caggagttcg tcctccgcgg cgtcgtcctg caatatgacg cgaatgccgt acccggctcc
     1081 cttgaggaga atccgcggtt cgttcccctg ctcgacgaac ttcttcaaca gcttttccct
     1141 ggtttccagg atattgaaca tggcgttcac tccctttcgc gttctccccg gtgatttctc
     1201 tcttgagaaa cagcctacac cttgacatag tgtgagagtc aagcggagga gcgcaatctt
     1261 ttgattccag agcgaatttt taaatgtggg agcggcgcgt aacgggcgac cttcgctcac
     1321 gggacttgtg gtccggggct gaatggcata tcatattgta atgaactgag agattccgtt
     1381 ccggccagag gagggagcgt tcatggaagt acagaacaac gagtggcagg tagtggtctt
     1441 ctttttggga gggcaggcgt tcgccatcag cgtggacaag accagggaaa ttcttcgctg
     1501 gccgggcagc cgtcctattc cggagtccca ccccgccatg atcggcatca catccgtccg
     1561 cggagaggta atgcccctgc ttgacctcag ggcgtacctc ggcatcacgc cgaaagtccc
     1621 gctggaatcg agcaaagtaa ttatcgcgga gttcaacgag agcaaactgg ggttcgtcgt
     1681 cgacggcgtc gaacgcattt acggaatcag cgccgcggag ttggattcca cattgacgag
     1741 cgcggtgctc ggcaagtcca ttctctacgt catcaagcga aacgagagca acgtcctgat
     1801 ccccgattac gaggcgatca tccaggcggc cgctccttcc ctcgacagaa gcctctccat
     1861 cgacctgaag aaggtcgcgg aactcgccgc gcctctcgga gaccttgacc ggtaccgtat
     1921 cctcgtcgcg gaggactcgc cgctcatccg gacgcagata tccgatgtgc tcacccaggg
     1981 cggcttccat aacgtgacgc tcgtcgccga cggcaaagag gcctgggagc gtctggcgat
     2041 gaagaacgaa cgctacgatc tcctcgtcac cgacgtcgaa atgccccgcc tcgacggcgt
     2101 cgccctcacc aagcaggtga aggagcaccc cgaactgaag cggatgccgg tcgtcgtctt
     2161 ctcctccatc atggtgcagg acgtccggct caaaatctcc ggcaccggcg ccgatgcaca
     2221 cgtcaccaag cccgaactcc cgcgtatggt cgagaaggtc tgccgcctcc tgctggactc
     2281 gaaggagaag gctgcggcga cgaagtaagg gctgtctgaa caccgtttcg gagggcgttc
     2341 gcgccctccg tttttatggg gaggaactat gaaagtcctg gtaagcgcat gcacgatagg
     2401 gtgcaattgt aaatataacg ggaaaaacaa caggaatgaa gcggtggtcg agtatctgcg
     2461 ggataaggaa gtcataagca tctgccctga agcgttggga aacatgggga cgccccgccc
     2521 gtgcgctgaa atcgtcgagg gcgtggcgat ggatgaacac ggcaagaatg tggatgcgga
     2581 atttcggaac gcggtcgagc ttgccttgga acaaataaag gatgaagcga tcgacctggt
     2641 gattctgcaa tcacgaagcc ccacgtgcgg agtgaaccgg atatacgacg gaacattttc
     2701 agggaagttg atccccggac aggggctctt cgcgcag
//
LOCUS       DAOSWW010000297         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28199_length_2731_cov_3.426383, whole genome shotgun sequence.
ACCESSION   DAOSWW010000297 DAOSWW010000000
VERSION     DAOSWW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28199_length_2731_cov_3.426383"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PK442_06790"
     CDS             complement(<1..1149)
                     /locus_tag="PK442_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87390.1"
                     /translation="MRIRRLPYIVVIIASILWTFAAPSFVEAWSQQPHSQINSEAITL
                     FVRRMAGKEKFRLGPFVDNALQQPLRGVAVTSSSLTASGFVSAEAEYSMRRWIVMGGD
                     WADEPHLYSSVRHFYDPLRITGVAYLTDQSWAHGHYDSPQIDARTWGLDHPDNPFTLF
                     TALAAYKAALEVRDDVPLPSSISAPHFKSTMNLVARDHEDQRKLHLSRAYRALGESMH
                     MLGDMTQPAHVRNDSHPADEPIEVATFPEHVRRAAANPFIDSRIRPFIASAGGTLQAP
                     AELFDQVARFTNSTFYSMDTIYDKPLGILPNNRQTPYPSPQFKDLVEVKTTVRGFVIP
                     RTVKKLYGVFDGKRVAMAQERLSFHWFDPDQTVFGGFDNLKEKTGRGIA"
     gene            complement(1181..1990)
                     /locus_tag="PK442_06795"
     CDS             complement(1181..1990)
                     /locus_tag="PK442_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87391.1"
                     /translation="MLWLLFLLLAALLPVSRNEARAQTEGGREARSPSNETLRERIAL
                     LEKALSFRGEGERMEQGGDFLGALLSFKRSHTLYPDPALERKIRELTAGEPSSPRPDL
                     QPLRDRIALLEELLAGLGTLDSLFGPEETPVSSGPVVAVPPEEEFTGSENPDWKNYPD
                     LPEEKAAIEESFDELRNALKEGDIERAVSVIDEDRREEYAALFAHKPEAMPSFAELLE
                     NAEMSFFSAAERADPATSTALRTSEYAVVLDGFTFYVRWIKVDGKWVLFDF"
     gene            complement(2262..>2731)
                     /locus_tag="PK442_06800"
     CDS             complement(2262..>2731)
                     /locus_tag="PK442_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87392.1"
                     /translation="KWRSLARTQAVLLLTDLDRAACPPGLISEWFGEHTRMPDRLLVR
                     VAVREAESWLLADHEAMRSLLDIHSGLPDAPDTLPDPKQTLLTLARQAPREVRLGLVR
                     QEGTRLRQALRYNSLLSEWVFTEWEPERAAKRSESLRRAIHRIKERALEFSGK"
BASE COUNT          549 a          838 c          826 g          518 t
ORIGIN      
        1 cgcgattcct ctcccggttt tctccttcag gttgtcgaaa ccgccgaaca ccgtttggtc
       61 cgggtcgaac cagtgaaaac tgagacgctc ctgcgccatg gcgacgcgct ttccatcgaa
      121 cacgccgtag agtttcttca cggtgcgcgg tatcacaaac ccccgcaccg tcgtcttcac
      181 ctccaccaga tccttgaact gcggcgaggg atagggcgtc tgcctgttgt tgggaagtat
      241 gcccaggggc ttgtcgtaga tggtgtccat cgagtaaaag gtcgaattcg tgaagcgggc
      301 gacctggtcg aagagctcgg ccggcgcctg gagcgtccct cccgcgctcg caatgaacgg
      361 gcggatacgc gagtcgataa acgggttcgc ggcggcgcgg cggacgtgct cggggaaagt
      421 ggcgacctcg atcggctcgt ccgcggggtg ggagtcgttc cgcacgtgcg cgggctgcgt
      481 catgtcgccg agcatgtgca tcgactcgcc gagggcgcgg tacgcccttg agagatggag
      541 cttcctctgg tcctcgtgat cgcgtgcgac gaggttcatg gtcgacttga agtgcggcgc
      601 ggagatcgac gaaggaagcg ggacgtcgtc gcggacttcc agcgcggcct tgtacgcggc
      661 aagggcagtg aagagcgtga acggattatc cgggtgatcc agcccccacg ttctagcgtc
      721 gatctgcggg ctgtcgtagt ggccatgcgc ccagctctgg tcggtcaggt acgccacgcc
      781 cgttatccgc agcgggtcgt agaagtggcg cacagacgag tagagatgcg gttcgtcggc
      841 ccagtcgcct cccatgacga tccagcggcg cattgaatac tccgcctccg cggagacaaa
      901 accggaggcc gtcagcgaag aacttgtcac tgcaacgccg cgcaggggct gctgcagcgc
      961 attgtcgacg aaggggccga gccggaactt ttcctttccc gccatccggc ggacgaaaag
     1021 agtgatcgcc tccgagttga tctggctgtg cggctgctgg ctccacgctt cgacgaagga
     1081 cggcgcggcg aacgtccaaa ggatggacgc gatgatgact acgatatacg gcagtctgcg
     1141 aatacgcacg gtactttccc tccttttccg gccggagacg ttaaaagtcg aagagaaccc
     1201 acttgccgtc gacctttatc caccggacat agaacgtaaa accgtcgagg acgacagcgt
     1261 actccgatgt gcgaagcgcc gtggaggtcg ccggatcggc gcgctccgcg gcgctgaaaa
     1321 aactcatctc cgcgttttcg aggagttccg cgaacgaagg catggcctcg ggcttgtgtg
     1381 cgaagagcgc ggcgtactcc tcccggcggt cctcgtcgat cacggagacg gcccgttcga
     1441 tatcgccctc tttcagcgcg ttccgtaact cgtcgaagga ctcctcgatc gccgccttct
     1501 cctcgggcag atccgggtag tttttccagt ccggattctc gcttccggta aactcttctt
     1561 ccgggggaac ggcgacaacc gggccggaag aaaccggcgt ctcctccggg ccgaacagag
     1621 aatcaagcgt accaaggccg gcaagcagct cctcgagcag ggctatcctg tcgcgcagag
     1681 gttgcagatc agggcgcggc gaagaaggct ctcccgccgt cagctcacgg attttccgtt
     1741 ccaatgccgg atcggggtag agcgtatgac ttcgtttgaa tgaaagcaac gcgccgagaa
     1801 agtcgccccc ctgctccatc cgctcgccct cgccgcggaa agagagcgcc ttttcaagca
     1861 gcgcgatacg ctcgcggagc gtctcgttcg acggagaacg cgcttcgcgt cccccttccg
     1921 tctgtgcacg cgcctcgttt cgggaaacgg gaagaagcgc cgcgagaaga agaaacagca
     1981 gccagagcaa gatacttccc ccccggcgca cgcgcgcccc gcgaaacacc cgggccggta
     2041 ctgcgctttt tccggaagac atgatctccg cacctccttc agaacacacg gaatgcaggc
     2101 aggattcctt ctgtaaaaag aggccgcgcc cgcaatttca tactgcgctt tgttgacgca
     2161 cagcttattc taccaagctt gaaatgtcaa acagggagga ctgcgatgaa ggctacaaga
     2221 aaatatggat tttcacttac ttgcaaatat ttatagagct ttcacttccc ggagaactca
     2281 agagcgcgct cctttattct gtgaatcgca cgccgcaaac tctcggaacg tttcgctgcg
     2341 cgctcgggtt cccattcggt aaaaacccat tccgaaagaa ggctgttgta tcgaagcgcc
     2401 tgccgcagac gagttccctc ttgccgcacc agaccgagac gaacctcgcg gggcgcctgc
     2461 cgtgcaagcg tgagcaacgt ctgtttgggg tcgggcagcg tatccggggc atcgggcaat
     2521 ccggaatgaa tatcaagcag agagcgcatc gcctcgtggt ccgcaagcag ccagctctcc
     2581 gcctcccgga cagcgacacg tacgagaagg cgatcgggca ttctggtgtg ctctccgaac
     2641 cattcggaga taagtcccgg aggacatgcg gcccggtcca ggtccgtcaa caacagaacc
     2701 gcctgagtcc gtgccaaact tcgccacttc c
//
LOCUS       DAOSWW010000298         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28215_length_2730_cov_3.574579, whole genome shotgun sequence.
ACCESSION   DAOSWW010000298 DAOSWW010000000
VERSION     DAOSWW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28215_length_2730_cov_3.574579"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PK442_06805"
     CDS             complement(<1..525)
                     /locus_tag="PK442_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOO87393.1"
                     /translation="MASPVTMPKLGLTMNTGVVSQWKKKEGEPVRKGEILMVVATDKL
                     TFDVESPSEGVLLKVLVPEGKDVPVGEVLAYVGAAGETVAGAAPEMPAAAPAAEEKKA
                     AAPSEVRSAPAGGIRATPLARKTAREAGIDLSLVAGSGPSGRIVRKDVDAAAASGGGA
                     VKASPVAAKMAAELG"
     gene            complement(596..1894)
                     /locus_tag="PK442_06810"
     CDS             complement(596..1894)
                     /locus_tag="PK442_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO87394.1"
                     /translation="MESLLREPAFWTIVLFLVPLLLRVPIAVALGSAAIFVVWNWDLG
                     YQMLSYSFFSGIAKFPLLAIPFFILAGVIMEKVGIAERIIALIKQLVGDMTGGLAVAT
                     VIVAAFWGAVSGSGPATVAALGLILIPGMAEAGYDKAFATAVVSVSAGLAIIIPPSIS
                     FIVYGDIMQQSVGTLFAAGVVPGIVVAVFLCVTVWLISRKHGYRGAPRGTSKEVLFAL
                     KDAFWGLFTPVIILGGIYGGVFTPTEAAAVAVFYGLFVGVFIYRSLTLRILYDILRET
                     VAGTSVVMIVVTCAGLYSWLGATVGLIDKIAALLLGISENPLVILLMINIILLFAGML
                     LDAISIAYVFLPILAPVIVTFGWDPIWFGVMMTVNMAIGQVTPPVAVNLYVGARISGL
                     TMEQITRPVIPLLVASIIALAVITIFPGITLYLPRLLGMM"
     gene            complement(1907..2377)
                     /locus_tag="PK442_06815"
     CDS             complement(1907..2377)
                     /locus_tag="PK442_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO87395.1"
                     /translation="MVKKLFDNFEELLGSLILIVMAVVTFANVITRYFIFYALSFTEE
                     ITIYLFVWLVMLGTAIAFKKGANLSMTFLYDKLPRKGKKFCFYVSAGMSLVFFGLLAY
                     LGYVQVMDEIDLAVTSPSLAIPIYYYTIALPVFSALIFIRVLQATMETLRKNEF"
BASE COUNT          640 a          821 c          812 g          457 t
ORIGIN      
        1 gcccagctcc gcagccattt tcgccgcgac cgggcttgcc ttgacagcgc cgccgcccga
       61 ggcggccgcg gcgtccacat ccttgcggac gatccggccc gacggtcccg aaccggcgac
      121 gagcgagagg tcaatccccg cctcacgcgc cgtcttacgg gcaagcggcg tggcgcggat
      181 tccgcccgcc ggagcgctgc gaacctcgga aggagcggcg gccttcttct cctccgccgc
      241 gggcgccgct gcgggcattt ccggagctgc gcccgcaact gtttcgccgg cggcgccgac
      301 gtaggcgagc acctcgccta cgggaacatc ctttccttca gggacgagga ctttcagcag
      361 aacgccttcg gagggcgatt ccacgtcgaa cgtcagctta tccgtggcga cgaccatcag
      421 aatctcgcct ttccggacgg gctctccctc cttcttcttc cactgggaga caacccctgt
      481 gttcattgtc agcccgagct tgggcattgt tacgggtgaa gccattcaaa aacccctcct
      541 tttgaggaac gaaagaacat acgctgtaca acgacaggca gatacgctgt ccgcgctaca
      601 tcatgcccag aagtcggggc aggtagagcg tgatccccgg gaatatggta atgacggcaa
      661 gcgcaatgat cgacgccacg aggagcggta tgaccggtcg ggtgatctgc tccatcgtga
      721 gtccgctgat tctcgcgccg acatagaggt tcaccgccac cggcggggtg acctggccta
      781 tggccatgtt gacggtcatc atcacgccga accagatggg atcccaaccg aaggtgacga
      841 taaccggcgc caggatcggg aggaagacgt aggcgatcga gatcgcgtca agcagcatcc
      901 cagcgaagag caggatgatg ttgatcatga gcagaatgac cagcggattc tcggagatgc
      961 ccagaagaag cgccgcaatc ttgtcgatga gtcccactgt ggcgccgagc caggaataaa
     1021 gccctgcgca ggtgaccacg atcatcacca cggaagtccc ggcgaccgtc tcccggagta
     1081 tgtcgtagag aatgcgaagc gtgagactgc ggtagatgaa gacgccgacg aacagaccgt
     1141 agaacaccgc aaccgccgcg gcctccgtgg gcgtgaagac ccctccgtaa atgcctccga
     1201 ggatgatcac cggtgtgaag agcccccaga aagcgtcctt cagcgcaaaa agaacttcct
     1261 tggaagtacc cctcggagcg cccctgtagc cgtgttttct actgatcagc cacaccgtga
     1321 cgcagaggaa aacggcgacg acgatgcccg gtacgactcc ggcggcgaag agcgtgccca
     1381 ccgactgctg catgatgtcg ccgtacacga tgaaggagat gctcggcgga atgatgatcg
     1441 caagccccgc ggacacggag acgaccgcgg tggcgaacgc cttgtcatac ccagcctccg
     1501 ccatgccggg gatgagaata agtccgagag ccgccaccgt cgccggaccg gagccgctca
     1561 cggcgcccca gaacgcggcc acgatgaccg tcgcaacggc aaggccgccg gtcatatcgc
     1621 ccacgagctg cttgatgagg gcgataatcc gctcggcgat gccgactttt tccatgatga
     1681 ctccggcaag aatgaagaag ggtatcgcaa gaagaggaaa cttcgcgatg ccggagaaga
     1741 agctgtacga aagcatctga tacccgagat cccagttcca gaccacgaag atggcggccg
     1801 aaccgagggc tacggcgatg gggaccctca acaggagagg gaccagaaag agaacgatcg
     1861 tccaaaatgc tggttcacga agcaaagact ccatctctcg tccctcctaa aactcgtttt
     1921 tgcggagcgt ttccatagtc gcctgcagaa cgcggatgaa gatgagcgcg gagaagacgg
     1981 gcagagcgat cgtatagtag tagatcggta tggcgagcga cggcgaggtg acggcgaggt
     2041 cgatctcgtc catcacctga acatacccca ggtacgccag aagaccgaag aacacgagcg
     2101 acatgccggc ggaaacgtag aaacagaact tctttccctt acgggggagc ttgtcgtaga
     2161 gaaaggtcat gctcaggttg gcgcccttct tgaaggcgat cgctgttccc agcatcacca
     2221 gccagacaaa aaggtagatg gtgatctctt ctgtgaatga aagcgcataa aagatgaagt
     2281 agcgggtaat aacgttggcg aacgtgacca ccgccatcac aataagaatg agagagccga
     2341 gaagttcctc gaagttatcg aataattttt ttaccattta ggccaatccc ttctgaagaa
     2401 aaaagactct gtgatttcct cccccacgcg tcccgcagac gccctcctcg aagacaaacg
     2461 aagaagcgtg cggggaggaa ggaagagaag gcccccgggc aaacgagccg agggctcggg
     2521 gggagccccg tccccccgag gtcgcaaaca aaagacgtct acttcgccac agccgccata
     2581 tccgcctcgg cggcgtcgac gatggcgtcg ccgatcttgg aacgccactc tttgacgacg
     2641 ggcgccgtct tgtcggcgaa cgccttcgtc tcttcagccg acaggatact cacggtcatg
     2701 ccgacattgg agagctcttt gtaccagtcg
//
LOCUS       DAOSWW010000299         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28308_length_2724_cov_4.172724, whole genome shotgun sequence.
ACCESSION   DAOSWW010000299 DAOSWW010000000
VERSION     DAOSWW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28308_length_2724_cov_4.172724"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PK442_06820"
     CDS             complement(<1..1075)
                     /locus_tag="PK442_06820"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOO87396.1"
                     /translation="MANVRKGALEGLRVLDLTRVLAGPFCTMMFADMGAEVIKIEEAG
                     KGDDTRQMGPFKNGESAYFMNLNRNKKGITLNLKNPKGKAIFLDLVKKADIVIENFRP
                     GTMEKLGLGYEELKKLNPAIIYAAISGFGHTGRYSQRPGYDIISQAMSGLMSTTGWPD
                     GEPTRTGTAMGDVLGGLSCAIGILSAVFNRVSTGLGQKVDVALVDSAVASLEIINMIY
                     LVEGRIPQRIGNRYESTYPYDSFRASDGSLVIGVANDKLWAQLCEAMDLQHLINDIRF
                     ARVRDRVANHAELKPLIEEWTTRFTVDEVCEKVSAAGVPCAPILNIEQVVNDPHIGGD
                     REMFVEMEHPVAGKLKVTGSHIKL"
     gene            complement(1123..1818)
                     /locus_tag="PK442_06825"
     CDS             complement(1123..1818)
                     /locus_tag="PK442_06825"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO87397.1"
                     /translation="MLKTHSFRHSTLGEEVASYLGKELLLSENYTPGSFIREEELAKA
                     LNVSRAPVREGIKILEGFGLLRTIPQKGAMVVDFSPEEVEELYDIRYALEEIVFREII
                     EKGLFTRDDYDIFRKKLDRMLLLGMNVDSKETILLEFSRIDMDFHTSLAEKSGRRWTL
                     RMLEMVYRQIQQAVLKDLAAENDMKALVDEHIRILDALLAGDLEELKKGRFFSYFQRR
                     IAPGREDRLASGV"
     gene            complement(1971..2558)
                     /locus_tag="PK442_06830"
     CDS             complement(1971..2558)
                     /locus_tag="PK442_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO87398.1"
                     /translation="MEAVSRCNTRRLVWTALLSALAAVLMYIDMALPIFPRFLKIDIS
                     DLPALVASFSYGPVAGVFTELIKNLIHMFSTSSAGIGEAANFLMGSALVVPAGLIYRT
                     NRTKKGAVAGLVAGTLCMTVTASFANYFILLPFYSHLMPLEKIISMSSAVIPAIHDKF
                     TLILYAVIPFNLLKGFLVSIVTIRLYKRISAMLCR"
BASE COUNT          552 a          746 c          777 g          649 t
ORIGIN      
        1 aaagcttgat gtggcttccg gtgaccttca gttttcctgc gacggggtgc tccatctcga
       61 cgaacatctc tctgtcgccg cctatatgtg ggtcgttcac gacctgttcg atattcagaa
      121 tcggcgcgca cggcacaccc gccgcggaga ccttctcgca gacttcgtcc actgtgaagc
      181 gggtggtcca ctcctcaatc aacggtttca gctcggcgtg gttcgccacc cggtcccgca
      241 cccgcgcaaa gcggatgtcg ttgatcagat gctgcaggtc catggcctcg cagagctgcg
      301 cccagagttt gtcgttggcc acgccgatga cgaggctgcc gtcggaggcg cggaaggagt
      361 cgtacggata ggtggactcg tagcggttgc cgatacgttg cggtatccgc ccctcgacga
      421 ggtagatcat gttgatgatc tccaggcttg ccaccgccga gtcgaccagc gcaacgtcga
      481 ccttctgtcc aaggcctgtg gagacgcggt tgaatactgc cgaaagaatg ccgatggcgc
      541 aggagagccc gcccaatacg tcgcccatgg ctgttcctgt gcgggtcggt tcgccgtcgg
      601 gccagccggt ggtgctcatg aggccgctca ttgcctggga gatgatgtcg taccccggcc
      661 gctggctgta gcgcccagtg tggccgaagc cggaaatagc ggcgtagatg atcgccgggt
      721 tcaatttttt cagctcttcg tatcccaaac cgagcttttc catcgtgccc gggcggaagt
      781 tctcgattac gatatcggcc ttcttcacaa gatcgagaaa gatcgccttt cccttggggt
      841 tcttcaggtt cagcgtgatt cccttcttat tccggttcag gttcatgaag tacgcgctct
      901 caccgttctt gaaagggccc atctgccggg tgtcgtcgcc cttgccggcc tcttcgatct
      961 tgatgacctc tgcgcccatg tcggcgaaca tcatcgtaca gaacggtccc gcgagcacgc
     1021 gggtaagatc gaggactcga agtccttcaa gcgctccttt tcgtacattt gccattctta
     1081 ttcctcccct tgagtgaatt cgttctttcc cgcgcaggtg tttcatactc cggaagccag
     1141 tcggtcttct cttccggggg cgattctcct ctgaaaatag ctgaaaaaac gtcctttttt
     1201 cagttcttcc agatcgccgg ccagcaacgc gtccagaatg cgtatatgct catctaccaa
     1261 agctttcata tcgttctcgg cggcaagatc cttcaacacc gcctgctgaa tttgtctgta
     1321 gaccatttcg agcattctca acgtccatcg tctcccggat ttctccgcga gggatgtgtg
     1381 gaagtccatg tcgattctgc tgaattcaag aaggatggtt tcctttgagt cgacattcat
     1441 tccaaggaga agcattctgt cgagtttttt cctaaagatg tcgtagtcgt cgcgggtaaa
     1501 cagccctttt tcgataattt cacggaagac gatttcttcg agagcgtaac ggatatcgta
     1561 gagttcttcc acctcttcgg gggagaaatc gacgaccatc gctcccttct gcggaatagt
     1621 gcggagaagg ccgaatcctt ccagtatctt gattccttcg cggaccggcg ccctgctcac
     1681 gttcaacgct ttcgcgagct cttcctccct tatgaaggag ccgggagtgt agttttcgga
     1741 gagcagcagc tctttgccga ggtaggaggc cacttcctct ccaagcgtac tgtgtctgaa
     1801 tgaatgtgtt ttgagcacgc cgaaacctcc ttcaggatgc gatatgttaa atgtcgacat
     1861 ttgagatatt gacatgatta tacagactgc ggtgaaggtg tcaagaaaaa acaaacccgc
     1921 cgcaaggcgg gtttgtcgcg tgatcgaggt gttctgtgtt caggagaata ctagcggcac
     1981 agcatggcgc tgatacgttt gtacaggcgg atcgtcacga tggagaccag aaatcccttc
     2041 aggagattga acgggatgac ggcgtagagg atgagtgtga acttgtcatg gatcgcgggg
     2101 atcaccgccg aggacatcga aataatcttc tcgaggggca tcagatgcga gtagaagggg
     2161 aggaggatga agtagttcgc gaaggatgct gtgaccgtca tgcagagggt tcctgctacc
     2221 agtccggcga cggcgccttt tttcgttcgg tttgtccggt agatcagccc tgcggggacg
     2281 acgagggccg agcccatcag gaaattcgcc gcctcgccga ttcccgcgga ggatgttgag
     2341 aacatgtgga tgaggttctt gatcagctct gtgaagactc cggcaaccgg gccgtaggag
     2401 aacgaggcga ccagcgccgg aagatcggag atgtcgatct tcagaaaacg gggaaatata
     2461 ggcagcgcca tgtcgatgta catcagcacc gcggcaagtg cggagagcag cgcggtccag
     2521 acgagacggc gcgtgttgca acgggatacg gcttccatag tatgatcaag tcctttcaaa
     2581 aaaagtagcc ccggacgcga cgcatccggg gcacgtgtgc agtcttcgac accctcttcc
     2641 atcctgactc cggtccgtcc ggaaggacat ccttacagtc ggctccggat tcgcaccgga
     2701 tcggcctttc ggctcgcggg cttg
//
LOCUS       DAOSWW010000300         2722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28339_length_2722_cov_3.482565, whole genome shotgun sequence.
ACCESSION   DAOSWW010000300 DAOSWW010000000
VERSION     DAOSWW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28339_length_2722_cov_3.482565"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK442_06835"
     CDS             <1..314
                     /locus_tag="PK442_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87399.1"
                     /translation="RLKALQDLLGEIHDCDVWLDFLPRFLEEEAARTLKYYGHVRVFP
                     PLKTGILAFLEKKRRLRGEDFDRFLDEWERLGKEAFWDNLNTAAETLRAYRGEEGADH
                     S"
     gene            311..1597
                     /locus_tag="PK442_06840"
     CDS             311..1597
                     /locus_tag="PK442_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOO87400.1"
                     /translation="MKIILLGDVHGNLPALEAVLEDGRRRGGDIVLNTGDYVGQGPFP
                     DETVSLLSSVESVAVAGNFDRNVLRAKQGKTGEKAELHLWNRGRLSRESRKYLKNLPR
                     TKKFLLAGKQFLLTHGSPEDIEENIGPDTPDERLCEIARLASSDIVLTGHTHAPLSKK
                     IGDTWFINPGTAGRPLGDDLRACYALLHIQPGYFRVNHYLVDYDMAKTADAALFRGMP
                     GDFASSFLPGRAVAGDIPMEDEAERILETTIPVHADHSRHVASIALALFDRLEELHGL
                     DREARFQLQIASTLHDIGWLKGQKEHHKVAMEFILGLDGVIPDERERILIASVARYHR
                     KADPDDSHPFFERLSPGDRETVRKLAAILRIADGLDWTHSSVVKGVECLVGDGAVTVL
                     CSTSGDAEAERARALEKGALFEKVFGKKLDIEWVRS"
     gene            1582..>2722
                     /locus_tag="PK442_06845"
     CDS             1582..>2722
                     /locus_tag="PK442_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOO87401.1"
                     /translation="MGPQLKSGHVVGLIDLGTNSVRLFVVRINENKSYTILNQHKIMV
                     RLGEGEFVKQRLKNEAMERTILVLGRFAEMARNLGAEEILAVATSATRDAENKDVFLE
                     RVRKEAGIDLKVISGLEEARLIYLGIASGMHLGDKTALFIDIGGGSTETIVGNQAEYF
                     HLDSLKLGAVRLASLFFLPGETDPVSPDRYALLQQYVRNASLRSVQRLREFPVDLVVG
                     SSGTLETLAEMAFRRGNKKQKGASAEKESSERTMTRRQLEELTKLLCSLSLEERKRLP
                     GMVPERADIIVAGASIIHTLMEDLELDKIRISTRSLRNGLLVDYLMRGKWGYLDAAFS
                     VREQSVLQLARSCGFHEEHSRHLGDLCMQLFDSGREAGLHSFGESA"
BASE COUNT          551 a          870 c          855 g          446 t
ORIGIN      
        1 agaggctgaa ggccctccag gacctccttg gagagattca cgactgcgac gtctggcttg
       61 atttcctccc ccgttttttg gaggaagaag cggcgcggac gctgaagtac tacgggcatg
      121 tccgggtctt tcccccgctg aagacgggca tcctcgcttt cctggagaaa aaaaggaggt
      181 tgcggggaga ggatttcgat cgttttctcg acgagtggga acgcctcggg aaggaagcgt
      241 tctgggacaa tctgaacacc gccgcggaga cgcttcgagc gtaccgcgga gaggaaggag
      301 cggatcattc gtgaagatca tactgcttgg agacgtccac ggcaatctcc ccgcgctgga
      361 ggccgtcctg gaggacggac gcagacgagg aggcgacatc gtgctcaaca ccggcgatta
      421 cgtcggacag gggccgtttc cggacgagac cgtctcgctg ctctcctcgg tggaatcggt
      481 ggccgtcgcg gggaacttcg accggaacgt cctccgcgcg aagcagggaa agacggggga
      541 gaaggccgaa ctgcacctct ggaacagggg acgcctttca agggaaagcc gcaagtacct
      601 caaaaacctc ccccggacga agaaattcct gctcgcgggc aagcaatttc tgctcacgca
      661 cggctccccc gaggacatcg aggagaacat cggcccggac actcccgatg agcgtctttg
      721 tgagatcgcc cgcctggcct cctccgacat cgtgcttacc gggcataccc acgcccctct
      781 ctcgaaaaag ataggcgaca cgtggttcat caacccggga acggccggac gcccccttgg
      841 agacgacctc cgtgcctgct acgcgctgct gcacatccaa cccggctact tcagggtgaa
      901 ccactacctc gtcgactacg atatggcgaa gacggccgac gcggcgctct tccgggggat
      961 gccgggggac ttcgcctcct cctttctgcc gggacgggcc gtcgcggggg atatcccgat
     1021 ggaggacgag gccgaacgca tcctggagac gaccattccg gtgcacgccg accacagccg
     1081 ccacgtggcg tccatcgccc tcgccctctt cgatcgcctt gaagagcttc acgggctcga
     1141 ccgggaggcg cgcttccagc tccagatcgc ctcgacgctc cacgacatcg gctggctgaa
     1201 ggggcagaag gagcaccaca aggtcgccat ggagttcatc ctcggactcg acggcgtcat
     1261 ccccgacgag cgggagcgca tcctgatcgc ctccgtcgcc cgctaccaca gaaaagccga
     1321 ccccgacgac agtcacccct tcttcgagcg tctctcaccc ggggacagag agaccgtacg
     1381 gaagctcgca gcgatcctca gaatcgccga cgggctcgac tggacccact cgtcggtcgt
     1441 caaaggggtg gagtgtctcg tcggcgacgg ggccgtgacc gtcctctgct cgacgtcggg
     1501 agacgccgag gcggagagag cgcgggccct ggagaagggc gcgctcttcg agaaggtctt
     1561 cggaaagaag ctggatatcg aatgggtccg cagctgaagt cggggcacgt ggtcggcctc
     1621 atcgacctcg ggacgaactc cgtgcgcctt ttcgtcgtgc gcatcaacga gaacaaatcc
     1681 tacacgatac tgaatcagca caagatcatg gtccgcctcg gcgaggggga gttcgtcaag
     1741 cagcgcctca agaacgaggc catggagcgg acgatccttg tgctcggcag attcgccgag
     1801 atggcgcgaa acctcggcgc ggaggagatc cttgcggtgg cgacctccgc cacgcgagac
     1861 gcggagaaca aggacgtctt cctggaacgc gtgcgcaagg aggcgggaat cgacctgaag
     1921 gtcatctccg gactggagga ggcccgcctg atctacctcg gcatcgcgag cggcatgcac
     1981 ctcggcgaca aaacggcgct cttcatcgac atcggaggcg gcagcacgga gacgatcgtc
     2041 ggcaaccagg cggagtactt ccacctcgac tccctcaaac tcggcgcagt ccgcctcgcg
     2101 tcgctcttct tcctccccgg ggaaaccgat ccagtctctc cggaccgcta cgcgctgctg
     2161 cagcagtacg tccggaacgc ctcgcttcga agcgtccaga ggctccgtga atttccggtc
     2221 gatctcgtcg tgggaagctc cggcacgctg gaaacgctgg cggaaatggc gttccggcgc
     2281 gggaacaaga aacaaaaagg cgcctccgcc gagaaggaaa gctccgaacg gaccatgacc
     2341 cggcgccagc tcgaggaact gacaaagctc ctctgctccc tctcgctcga agagcggaaa
     2401 cgtcttcccg gcatggttcc tgaacgtgcg gacatcatcg tcgccggcgc ctccatcatc
     2461 cacacgctga tggaggatct tgagctggac aagatacgga tcagcacgcg aagcctccgg
     2521 aacggtctgc tggtggacta cctgatgcgc ggaaagtggg ggtatctcga cgccgccttc
     2581 tcggtgcgcg aacagagcgt cctccagctt gcgcgatcat gcggcttcca cgaggagcac
     2641 tcgcgccacc tcggagacct ctgcatgcag ctcttcgaca gcgggagaga ggccgggctg
     2701 cactccttcg gcgagagtgc ga
//
LOCUS       DAOSWW010000301         2721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28352_length_2721_cov_4.762566, whole genome shotgun sequence.
ACCESSION   DAOSWW010000301 DAOSWW010000000
VERSION     DAOSWW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28352_length_2721_cov_4.762566"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PK442_06850"
     CDS             <1..929
                     /locus_tag="PK442_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO87402.1"
                     /translation="MAIPFLVSLGYDKVYSAALVATAGSLGVIIPPSIPFVTYGVITG
                     VSIGSLFIAGIIPGLVIGLSLMGYALFYAKVHGEDRKLISEKVKALRSGGLLRLFRES
                     FWALLTPVIILGGIYSGIVTPTEAAAVSVFYALLVCLFVYKSIAFKELGTILRDTVKS
                     YAPIVVLLSLAIVFGRVLALLQAPAVLSNFILNNFAGNKYIFLIALNIILLVLGMFMD
                     VGPAIAILAPMMLAAATALGISPVHLGVIMVINLAVGMVTPPFGVDLFVAAPLINEHV
                     MKVGIKAIPFIIAFIFALILITFIPQMSLLFL"
     gene            1055..2122
                     /locus_tag="PK442_06855"
     CDS             1055..2122
                     /locus_tag="PK442_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO87403.1"
                     /translation="MSGIMEEHNLKKEGYEMKQVRVFSLLLGIAFVVSGMFVCAGPLA
                     AQEYTLQLAGAYPSTGETPRALATEKIKELIEKKSNGKIKVQIYLDNQLGGDREILEG
                     CQLGDIAMVSQTTAPQVGFIPELAIFDIPMLYENLDVARKALSGTFRAKLEEWYDRAG
                     LKLLLFEPIYYRETTTSKPINTVADFGGVKIRTMENEYHMAFWKALGANPTPLSFAEL
                     YVALQQGTVDAQENPYEIIWSSKFYEVQKYLVNTHHIAFILSININKGIYDGMSDEYK
                     KIIDESMKEASEYLFDLAKAKNDAMLASLKQSGMTIIDPDPALQAELKKAAAETEKLI
                     RKNVGDAVVDEVLKSAEEAKK"
     gene            complement(2224..>2721)
                     /locus_tag="PK442_06860"
     CDS             complement(2224..>2721)
                     /locus_tag="PK442_06860"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HOO87404.1"
                     /translation="TTLQEGDFLWRLSSANVGESGPFSTFPGYDRVLFLLSGNGLELT
                     VNGETVPLRSPFDSLRFRGEHDASCMLTDGPVTDFNIFCRREILTCEYRHLFSKKDIE
                     RLFLPGDTTILLCLQGEATAEIEGKHPLKEMEMLVVTDHSWGQPLTLQYGEKARLLFI
                     TLTKR"
BASE COUNT          640 a          702 c          710 g          669 t
ORIGIN      
        1 gcatggcaat cccctttttg gtctcactgg ggtatgacaa ggtctacagc gccgcgctgg
       61 tggccacggc gggaagcctc ggtgtcataa ttcctcccag cattcctttc gtcacctacg
      121 gagtgatcac cggcgtatcc atcgggagtc tctttatcgc ggggatcatt cccggactcg
      181 tcatcggact cagcctcatg ggctatgctc ttttctacgc caaggtgcac ggtgaagaca
      241 gaaaactcat ttctgaaaaa gtgaaagccc ttcgttcagg cggtttgttg cggctgttca
      301 gagagagctt ctgggctttg ctcactccgg tcatcattct cggcgggatc tacagcggga
      361 tcgtgacccc cacggaggcc gccgccgttt ccgtcttcta cgcccttctg gtatgtcttt
      421 tcgtctacaa aagtattgca ttcaaggaac tggggacaat actcagagac actgtcaaat
      481 cctacgcccc catcgtcgtc ctcctgtccc tggctattgt gttcggccgg gttcttgccc
      541 tgctacaagc ccctgccgtt ttgagcaact ttatcctgaa taattttgcc gggaacaagt
      601 atattttcct catcgcgctc aatattattc tcctggtcct gggcatgttc atggacgtgg
      661 gaccggccat cgccattctt gcgccaatga tgctcgccgc cgccactgcc ctcggtatca
      721 gccccgttca cctgggagtt atcatggtga tcaaccttgc agtcggcatg gtcacgcctc
      781 ccttcggcgt ggatcttttt gtggcggcgc cgcttattaa tgagcatgtg atgaaagtgg
      841 gcataaaagc aatccccttt atcatcgcgt tcatctttgc gctcattctg attaccttta
      901 taccgcagat gagcctcttg ttcctgtagg atagggatct ggacggtcgt ttttttgaca
      961 atacaaagcg ggagtttttc atccattcta cgctctcaag ggaggtgaaa aagacaaaga
     1021 ccttttcagc tcgatagaga gaaggccgtt cggtatgtcg ggaatcatgg aagagcacaa
     1081 cttgaaaaag gaggggtatg aaatgaagca ggtcagggtg ttttcgcttc tattgggaat
     1141 tgcttttgtt gtgtcgggaa tgttcgtgtg tgcaggtccg ctggcggccc aggaatatac
     1201 gctgcagttg gccggggcct atccatccac cggagaaact cccagagcgc tggctacgga
     1261 aaagatcaag gagcttatcg agaagaaatc gaacggcaag atcaaggtgc agatttacct
     1321 cgataatcag cttggcggcg accgggagat actcgaagga tgccagctgg gcgacattgc
     1381 catggtctct cagacgacag ctccccaggt aggattcata cctgagctcg ccattttcga
     1441 tatccccatg ctgtatgaaa acctggatgt ggcgaggaaa gcgctttccg ggacgttccg
     1501 ggcaaagctc gaggaatggt acgacagggc gggactcaag ctcctccttt tcgaacccat
     1561 ctattaccgg gaaaccacca ccagcaaacc catcaatacc gttgccgact ttggcggagt
     1621 caagatccgc accatggaga acgaatacca catggctttc tggaaagctt tgggggccaa
     1681 tcctacccct ctgagcttcg cggagctgta cgtcgctctt cagcagggaa ccgtcgatgc
     1741 tcaggaaaat ccctacgaga tcatatggtc cagcaaattc tacgaagttc aaaaatacct
     1801 cgtcaatacc catcacatag cgtttattct gagcatcaac atcaacaagg gtatctatga
     1861 tggcatgtcc gatgaataca aaaagatcat cgacgaaagc atgaaggaag cgtcggagta
     1921 tctttttgac cttgccaagg cgaaaaatga cgcgatgctg gcttccctga aacagagcgg
     1981 gatgaccatc atcgatcctg atcccgccct tcaggcggaa ttgaaaaagg ctgctgcgga
     2041 aaccgaaaag ttgatccgta aaaatgtagg ggacgctgtc gtcgatgaag tgttgaagtc
     2101 agccgaagaa gcgaaaaaat agcgattcgc tccttgtttt tctgaaagaa gaagcccggg
     2161 tcgttgggat ccgggcttct tctttcagct atttcagcgc atggagtttt tccttacacg
     2221 tttttagcgt tttgttaaag taatgaaaag aaggcgtgcc ttctccccgt attgaagggt
     2281 caacggctgt ccccaagagt ggtccgtgac gacaagcatt tccatctcct tcagaggatg
     2341 ctttccttca atctccgccg tagcctctcc ttggagacaa agaagaatcg ttgtgtcccc
     2401 gggaagaaaa agacgttcaa tatccttctt cgagaagagg tgacggtatt cgcacgtgag
     2461 gatctccctc ctgcagaaaa tgttgaaatc cgtcaccgga ccgtcggtca gcatacagga
     2521 cgcgtcgtgt tcgcctctga accgaagcga gtcgaaggga gagcggagcg ggactgtctc
     2581 tccgtttacg gtgagttcca ggccgtttcc ggagaggagg aagaggacac ggtcgtatcc
     2641 cggaaacgtt gaaaacggac cggattcgcc cacattcgcg gaactcagcc gccaaaggaa
     2701 atctccttct tgaagcgttg t
//
LOCUS       DAOSWW010000302         2721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28360_length_2721_cov_2.518380, whole genome shotgun sequence.
ACCESSION   DAOSWW010000302 DAOSWW010000000
VERSION     DAOSWW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28360_length_2721_cov_2.518380"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /gene="dnaX"
                     /locus_tag="PK442_06865"
     CDS             <1..1632
                     /gene="dnaX"
                     /locus_tag="PK442_06865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOO87405.1"
                     /translation="VVEIDGASNNGVEEIRELKTHVSLTPFSAKWKVYIIDEVHMLSI
                     SAFNALLKTLEEPPPFVVFILATTEPHKVPVTIRSRCQHIPFHRIELRAICSRLEAVA
                     EKEGVPADPRALREIARHADGALRDALSLMEQALSLGNGEVSMEAADRLLGGGTLSDL
                     ERWIASLKENNVQPFLFLEEMFQKGASPQRVVEGVFLLFRNLWAVKRWGRPVLEALSL
                     AGSEMEFLCEEAASWKESQLSEMMLFCSKLIPQVRAGLRSDVLSGLLAAKGLECRSFA
                     EGPRANVKEEAPALRPEPPVVSPARPAQRVEDVTEAGIRSAPPFELPSKPVALPSKTE
                     ASSRLPAMIAEPSPEESVPFEGGPSEAGWRELEKRLFEKNLPLYCLLVGSSIAVEDNV
                     LEIRFPEEAGYCFTVLSSERNCHILASEAKKLLGPQTELFLLWRGDRRPCSGNGGTKD
                     DEDSPSWNEPASPPYLFKPPEDLQEKENERSLSGTAEGGEPPRSSGAAAAKEAGLPFE
                     GLVNEVLKWSEGEVILVKREEHEEEPPPAGETEQI"
     gene            1652..>2721
                     /locus_tag="PK442_06870"
     CDS             1652..>2721
                     /locus_tag="PK442_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO87406.1"
                     /translation="MTRQFVHLHVHTEYSLLDGAIRCGDLAAKAVEFGMPAVAMTDHG
                     AMYGTVEFYDKCTAAGIKPIIGCEVYVDPEGHTSREKRGKNHHLILLAENNEGYRNLV
                     KLCSIAYTDGFYSKPRIDHALLAQYKNGLIVSSACLAGEIPSLILEGREKEALERAFL
                     YRDIVGKENFFLEVMYNAIPEQAVANKALIRMAREHGFPLIATNDAHYLLKEDYDWHE
                     ILLCVQTKSSLDDENRMSFSSNDFYLRSPEEMDSIFGAELPDALDNTVAIAERCNVTL
                     PLGERDYKLPRLDLPEGETLESNLEKEAIVGLSERVGDPVPEEYRKRLDFEIGVINSM
                     GFAGYFLIVAGIIKAAKKRKIP"
BASE COUNT          573 a          810 c          829 g          509 t
ORIGIN      
        1 gtcgtcgaga tcgacggagc ctccaacaac ggagttgagg agatacgcga gctcaagacg
       61 cacgtttctc ttactccgtt ttccgcaaaa tggaaggtgt acatcatcga cgaagtgcac
      121 atgctctcca tctcggcctt caacgcgctg ctgaaaacac tcgaggagcc gcctcccttc
      181 gtcgtcttca tcctcgcgac cacggagccg cacaaggtgc cggtgacaat acgttcccgg
      241 tgccagcaca tccccttcca caggatagag cttcgggcca tctgctccag gcttgaggcg
      301 gtcgcggaga aagaaggggt tcctgccgat ccgagggcgt tgcgcgagat cgcgcggcac
      361 gccgacggag ctttgcggga cgcgctctcc ttgatggagc aggcgctctc gctcggtaac
      421 ggcgaggtct ccatggaagc cgccgatcgt ctgctcggcg gagggacgct ctccgacctc
      481 gaacggtgga tcgcctccct gaaggagaac aatgtccaac cgtttctctt cctggaggag
      541 atgttccaga aaggagcatc tccgcagaga gttgtcgagg gcgtctttct tctcttccgc
      601 aacctctggg cggtgaaacg atggggcagg ccggttcttg aggcgctttc actcgccggg
      661 agcgagatgg agtttctgtg cgaagaggct gcgtcctgga aggaatcgca gctctccgag
      721 atgatgctct tctgttccaa actgatcccg caggtgcgcg cagggcttcg ctcggacgtc
      781 ctctccggcc tgctggccgc gaaaggactt gaatgccgca gctttgcgga agggccccgc
      841 gcgaacgtga aagaggaggc gcctgcgctt cgtcctgaac ctcccgttgt ttctcctgcc
      901 cgcccggcgc aaagggtcga ggacgttact gaagccggga ttcgcagcgc gcctccattt
      961 gaacttccat caaaacccgt tgcacttcca tcaaaaaccg aagcgtcgtc gcgcctcccc
     1021 gccatgattg cggagccctc cccggaagaa tccgttccgt tcgaaggcgg accgtccgag
     1081 gcggggtgga gagagctgga gaagcgtctc ttcgagaaaa acctgccgct ctactgcctt
     1141 cttgtcggct cgtcgatcgc tgtggaggac aacgtcctgg agattcgttt cccggaggag
     1201 gccggatact gcttcaccgt gctttcctcg gagagaaact gtcatatcct cgcatccgag
     1261 gcgaagaagc tgctcgggcc gcagacggaa ctctttcttc tttggagagg cgaccgccgt
     1321 ccgtgttcgg gaaacggcgg aacgaaagat gatgaagact cgccgtcctg gaacgaaccg
     1381 gcatcaccgc cgtatctttt taaaccgccc gaagaccttc aggagaaaga aaacgagcgc
     1441 tccctctccg gaacggcgga gggcggcgag cctccccgtt cgtccggagc cgcggcggcg
     1501 aaggaggcgg ggctcccctt cgaggggttg gtcaacgaag tgctcaagtg gtccgagggc
     1561 gaggtcatcc ttgtaaagcg ggaggagcat gaagaggagc cgcctcctgc gggagagacg
     1621 gagcagatat gaaacgggga atttcaagac tatgacaaga cagtttgtcc atttgcacgt
     1681 tcatacggag tacagtcttc ttgacggcgc aatccggtgc ggcgatctcg ccgccaaggc
     1741 cgtcgaattc ggcatgcccg ccgtggcgat gacggaccat ggcgcgatgt acggaaccgt
     1801 cgagttctac gacaagtgca ctgcggccgg aatcaagccg atcatagggt gcgaggtcta
     1861 cgtcgatccc gaagggcaca catcgaggga gaagcgcgga aagaaccacc acctgatcct
     1921 cctggccgaa aacaacgagg ggtaccgcaa cctcgtcaaa ctctgttcca tcgcctacac
     1981 cgacggtttt tacagcaagc cccggataga tcatgcgctg ctcgcgcagt acaagaacgg
     2041 gctgatcgtt tcgtccgcct gcctcgccgg ggagatcccg tcgctgattc ttgaggggcg
     2101 tgaaaaggaa gccctggagc gcgcgtttct ctaccgggac atcgtgggga aggagaactt
     2161 tttccttgag gtcatgtaca acgccatccc ggaacaggcc gtcgcgaaca aggcgctgat
     2221 caggatggcg agggagcacg gcttcccgct catcgcgacc aacgacgcgc actacctgct
     2281 gaaagaggac tacgactggc acgaaattct gctctgcgtc cagacgaaga gcagcctcga
     2341 cgacgaaaac aggatgtcgt tctcaagcaa cgacttctac ctccggtctc cagaggagat
     2401 ggactccatc ttcggcgcgg agctccccga cgcgctcgac aacacggtag ccatcgcgga
     2461 gcgctgcaac gtgacccttc ccctgggaga acgcgactac aagctcccgc gcctcgatct
     2521 tcccgaggga gaaacgctcg agtcgaacct cgaaaaagag gcgatcgtcg gcctgagcga
     2581 gcgcgtcggc gatcctgtac ccgaggagta cagaaagcgt ctcgattttg agatcggcgt
     2641 gatcaattcg atggggttcg cgggctactt cctgatcgtc gcgggcatca tcaaggccgc
     2701 gaagaagcga aagattccga t
//
LOCUS       DAOSWW010000303         2719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28389_length_2719_cov_4.611486, whole genome shotgun sequence.
ACCESSION   DAOSWW010000303 DAOSWW010000000
VERSION     DAOSWW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28389_length_2719_cov_4.611486"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PK442_06875"
     CDS             complement(<1..331)
                     /locus_tag="PK442_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOO87407.1"
                     /translation="MDGKCLFCEMASGAVHVEKVFEDDAVFVIRDISPQAPHHLLVIP
                     KEHVESAADVQNPEVWSKVLDCAVRIASSFGLDNEGYRLVVNCGAKAGQTIPHLHIHL
                     LAGRSFRW"
     gene            complement(360..1352)
                     /locus_tag="PK442_06880"
     CDS             complement(360..1352)
                     /locus_tag="PK442_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOO87408.1"
                     /translation="MSVVIELTDCFEILGVQPGAHLREIRSAFRRLALSCHPDIAGPQ
                     KAKQFETIAAAYAQLKSATPAQISESLKKKKSHGDAGKGSPFSWKKTPPRRPKNDRRE
                     EAERESSKRVQDLLLEKALVEAELSMARILEKATRKDEKRTVSSLSKRLLSTHPGVRV
                     LALGALAGRPVEKNVFSSLIEMLRMWSPDDDTVEHLLLLDFSEEQKGDIAAALSARSL
                     LLSEASALALLRWASILPVKMSVVRKLLSHPSPRVLSQALSRWSEKSLPDDLTLIRLL
                     KREEDEVLAPLLRILKDRKVPVWAYGRVKMLAENHVSSAVRVWAQSIVRAQNLV"
     gene            complement(1355..>2719)
                     /locus_tag="PK442_06885"
     CDS             complement(1355..>2719)
                     /locus_tag="PK442_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO87409.1"
                     /translation="STTWWDMKRKLGSPWRARCGRASYTAEDVLVPLLTLLREDAEAF
                     LGTFISSCVLTVPAFFSFAERSAMGRTARSSGFSSVRIVNEPTAAALAFGIMGKFLIL
                     DYGAGTVDISVVESEGGVWQVLESAGLPASGGRDFDILLASSLNEKMKLPRLEDNSAL
                     WRALLSEAESLKIALSSCASCEWTPPAGVSCPSLRVTREEFEDLIRPSIEQVTTAVRR
                     LWMRFKPSKLLLVGGGSRIPLLRALLEERVARPEHLNQCPDESVVTGAALYGISPTKE
                     RLLIDVLSQDLGILAADGTPVTLLEKGLHLPVRAERHFYSVGNGPFTMSVFQGEGSSR
                     RIIASVQAPEARKDEEITLSFSVDSDGLLRIDIIRSDGRVSSIAPLELGEGVPPSPTE
                     TTEEAKGLERRFARLSVSLSPAQQARGAALLRTMKTLGDGDYSSEAFDSLERMISEME
                     RVVR"
BASE COUNT          650 a          796 c          716 g          557 t
ORIGIN      
        1 gccagcggaa actacgtccc gccaacaggt gaatatgaag gtggggaatc gtctgtcccg
       61 cttttgctcc gcagttgacc acaagcctgt atccctcgtt gtcaagacca aaggatgaag
      121 cgatgcggac ggcgcaatca aggactttcg accacacttc ggggttttga acgtcggcag
      181 ccgattcaac atgctctttc ggtattacga gaagatgatg cggagcctga ggggagatat
      241 cgcgaatcac aaatacagca tcatcctcga agactttttc cacatgcact gctcccgaag
      301 ccatttcgca gaaaagacat tttccgtcca aaaatcccac ctcacaacgt gacagtattc
      361 tataccagat tttgagcgcg cacaatagac tgcgcccaaa ctcgtacggc agatgacaca
      421 tgattttcag cgagcatctt cacccggcca tacgcccata cgggaacttt cctgtctttg
      481 agaatccgca acagcggcgc caagacttcg tcttcttctc tcttgagcaa ccgaatgagc
      541 gtcagatcgt cgggaagcga cttctccgac catcgggaga gcgcttgcga aagaacccgc
      601 ggagaagggt gggacaaaag ttttcgtaca acggacatct tcacgggcag aatagacgcc
      661 caccgcagca gagcgagcgc ggaagcctcc gagagcagga gacttcgtgc gctcaaggcg
      721 gcggcaatat cccccttctg ctcctcggag aaatccagga gaagaaggtg ctccactgta
      781 tcgtcgtcgg gagaccacat tcgaagcatc tctatcaagg aggagaaaac gtttttctcc
      841 acagggcgtc cggcaagagc tccaagcgca agcacacgca ctccggggtg agtgctcagg
      901 agacgtttcg acagcgatga aactgtcctt ttttcatcct tccgcgtcgc tttctcaaga
      961 atcctggcca tggagagctc cgcttcgacc agcgcctttt cgagaagcag gtcctgaacc
     1021 cgtttcgagc tctctcgttc cgcctcttca cgccgatcgt tcttcggccg ccggggagga
     1081 gtttttttcc acgaaaaggg cgaacctttt ccggcgtccc catgcgactt cttttttttc
     1141 aacgactcgg aaatttgcgc gggagttgcg gacttcaact gcgcgtaggc tgcggcgata
     1201 gtttcgaact gtttcgcctt ttgcggtccg gcaatatccg gatggcagga gagcgcaagc
     1261 ctgcggaacg ccgagcggat ttccctcaga tgcgctccgg gctggacccc tagtatctcg
     1321 aaacaatccg tcaactcgat aacaacgctc atttctatcg cacaaccctc tccatttccg
     1381 aaatcatccg ttccagcgag tcaaaagcct cggaggaata atctccgtct ccaagggttt
     1441 tcatcgtgcg cagcaacgcc gctcctcgcg cctgctgcgc cggggaaagc gatacggaaa
     1501 gacgcgcaaa ccgcctttcg agccccttcg cctcctcggt cgtctccgta ggtgaaggag
     1561 ggacgccctc tccaagctca agaggcgcga tagaggaaac tcttccgtcg gaacgtatta
     1621 tatcaattct taagagcccg tccgagtcta ccgagaagga gagcgttatt tcttcgtctt
     1681 ttctcgcctc gggagcctgt acggaagcaa tgatccttcg tgaggagcct tcgccctgga
     1741 aaacgctcat agtgaacggg ccattgccga cactgtagaa atgcctttcc gcacgaacag
     1801 gaagatgcaa gcctttttcc aggagcgtca ccggagtacc gtcggcagcc aatattccca
     1861 agtcctgcga gaggacgtcg atgaggagac gctcctttgt aggagatatg ccgtagagag
     1921 cagcgcccgt aacgacggac tcgtcggggc attgattgag atgttcaggc ctggcgacac
     1981 gctcttccag gagagcgcga agaagcggta tcctgcttcc tccacccacc agcaggagct
     2041 ttgaaggctt gaacctcatc cagagacggc gcaccgccgt cgtcacctgc tcgatggatg
     2101 gacgaatcag atcttcgaac tcctcgcgcg ttacgcgaag agaagggcag gaaactcccg
     2161 ccggaggcgt ccattcacac gaagcgcagg aggaaagagc aattttaaga gattccgctt
     2221 cagaaagaag cgctctccac aaggcggagt tgtcttcaag acgcggcagc ttcatcttct
     2281 cgttcaaact ggatgcaaga agaatgtcaa aatcccgccc tcccgaggca ggcaaaccgg
     2341 cgctctcaag cacctgccat acgccgcctt cgctctccac aacagaaatg tccacagtcc
     2401 ccgcgccgta atcgaggatg agaaatttcc ccattatgcc gaatgcaagc gcagctgccg
     2461 tcggttcgtt tactatgcga acggatgaaa agcccgagga acgcgccgtt cttcccatgg
     2521 cagatcgctc cgcgaagctg aaaaaagcgg gaacggtaag aacgcaggag gagataaagg
     2581 ttccgaggaa cgcctcggcg tcctcccgca acaacgtcag cagaggaaca agcacgtctt
     2641 ccgccgtgta ggaagcgcga ccgcatcgtg cccgccatgg agaaccaagt tttcgtttca
     2701 tgtcccacca ggttgtgga
//
LOCUS       DAOSWW010000304         2715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28474_length_2715_cov_2.616917, whole genome shotgun sequence.
ACCESSION   DAOSWW010000304 DAOSWW010000000
VERSION     DAOSWW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28474_length_2715_cov_2.616917"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1445)
                     /locus_tag="PK442_06890"
     CDS             complement(213..1445)
                     /locus_tag="PK442_06890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO87410.1"
                     /translation="MNGEQTIPELEERIVFLTMERRAAMEALEVALDISSFTASLTGI
                     ESVEEILRESAVKIRALLPFEALAFFLADEDDDMALAPAYADPPEKMHVIEQEKKPLI
                     DDRTVAWALRRKKPVIVATQDRSGRIFLHALASPSRVLGLFIGILGKEEEAQQTPSYP
                     DFLSIVLSATSGVIGTNMLLRKINLLNLALQEKVHFLEKSKEELSLYRNNLEREVAAR
                     TKELARRNEDLRREIVERKRMESEIRYRAWHDALTGLANRELFHSRLLEAVAKGEPIA
                     VFFMDLDGFKNINDTLGHDSGDSLLQEAARRIAREMGEENTVARMGGDEFTVIISFPT
                     SRELLAECAERLLHGISEPISLEGASVSITASIGISRFPEDGATTQDLMKCADVAMYK
                     AKERGKNRYCFWSDIRGK"
     gene            1607..1738
                     /locus_tag="PK442_06895"
     CDS             1607..1738
                     /locus_tag="PK442_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87411.1"
                     /translation="MSEVLRRKGRVLSNASLRPLFEKTGAGKALLQALRATKYGGGL"
     gene            1738..2256
                     /locus_tag="PK442_06900"
     CDS             1738..2256
                     /locus_tag="PK442_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOO87412.1"
                     /translation="MDYVGLLLIFCARIVDVSCGTVRILFLVRGRRALAAMIGFVEVM
                     IYLTALGHIIGGGREMNWIQMVVYCAGFSTGNFIGSLLEEKLMNAYVFLELIMDRTAE
                     TKEMIDNIRADGFGATVIYGRGRDGVRTIVKIICRRRDVPVIVAHTHNKGFICISDVK
                     GCSGGQFRFQRK"
     gene            2302..>2715
                     /locus_tag="PK442_06905"
     CDS             2302..>2715
                     /locus_tag="PK442_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87413.1"
                     /translation="MKDSLGVVRERNVGEIGGRQAGTCPEGYCEKTLRLEKELTRWKR
                     EEAVLRDIERRYLALLDSPLLILLILSEGRVLFMNRRGEEFFGFALRERPRFLLSEYA
                     ASGYASSVESLFTPGSDGAVRGGRLDFAVRSESGRE"
BASE COUNT          558 a          683 c          828 g          646 t
ORIGIN      
        1 ggcgaagctc cgtgaaaagc accaggttgt catcgtggga gagaagaaag aagaggccaa
       61 agtagaggca aaagaagagg caaaagaaga gcccaaagaa gaggccaaaa aagaagagaa
      121 gaagtaattc aaagctgcct cgtagatttg gagaataagg gcagggggcc gtgcagcatc
      181 ggttcccgcc ctttttttcg ctcacgttcc ggtcattttc ccctgatatc ggaccagaag
      241 cagtacctgt tttttccgcg ctctttggct ttgtacatgg ctacgtctgc gcatttcatc
      301 aaatcctgcg tggtggcgcc gtcctccggg aagcggctga tgccgatgct cgcagtgatg
      361 gagacggagg ccccttcgag gctgatcggt tccgatatgc catgcagcag cctttcggcg
      421 cactcggcga gcagttcccg cgatgtggga aaggagatga tcactgtgaa ttcatctccg
      481 cccatccgtg caacggtatt ctcttctccc atttcccggg caatcctcct ggcagcttct
      541 tgcagcaggc tatcgccgct gtcgtgtccg agggtgtcgt tgatgttctt gaagccgtcg
      601 agatccataa agaagacggc aatcggttct ccctttgcga ccgcttcgag caggcgggag
      661 tgaaagagtt ctctgttcgc aaggcctgta agcgcgtcgt gccacgcgcg atagcggatc
      721 tccgattcca ttcgtttcct ttcgacaatt tcccggcgaa ggtcttcgtt tcttctggcg
      781 agttcctttg ttcgtgcggc tacttctctt tccaggttgt tgcgatagag cgacagctct
      841 tccttcgact tttccagaaa gtgaactttt tcctggagcg cgagattgag cagattgatt
      901 tttcgaagaa gcatgttcgt gccgatcacg ccggatgttg ccgacaggac gatggagaga
      961 aagtcggggt aggaaggcgt ttgttgtgct tcttcttcct tcccgagaat cccgataaag
     1021 aggccgagta ctcgcgaggg cgaggcgagc gcatgaagga aaattctgcc ggagcggtct
     1081 tgcgttgcaa cgatgaccgg tttctttctg cgaagcgccc acgcgacggt gcggtcgtct
     1141 atgagcggtt ttttttcctg ttcgatgacg tgcatttttt ccggcggatc ggcgtacgcg
     1201 ggagcgagcg ccatgtcgtc gtcctcgtcg gcgaggaaga atgccagcgc ctcgaacggg
     1261 aggagcgcgc gtatttttac cgcggactcc cggaggatct cttccacgga ctctatgccg
     1321 gtaagcgacg cggtgaagga gctgatgtcc agggcgacct caagcgcctc cattgcggcg
     1381 cgacgctcca tggtgagaaa aacgatccgt tcttcgagct cggggatggt ctgttcgccg
     1441 ttcatagcgt ctctcctttt acgcgaaaga attgcgtttt tgctcatttg ccgtttatta
     1501 tactctgccg gagaggtatg ataggatagt aacggtatgt tgtatcaaaa attgctcgtc
     1561 ccggcatgca ctgagtgtct ttaccgggaa gcaaggaggt ttcggaatgt ctgaagtgtt
     1621 gcgaaggaag ggacgcgttc tttcaaacgc ctctcttcgt cctctctttg aaaagaccgg
     1681 cgccgggaaa gctcttctgc aggcgctccg ggcgaccaag tacggaggag ggctctgatg
     1741 gattatgtcg gacttcttct tatcttctgc gcccgtattg tcgacgtaag ctgcggaacg
     1801 gtgcggattc tcttcctcgt cagagggaga agagcgctgg cggcgatgat cggttttgtc
     1861 gaggtgatga tctatcttac cgcccttggg cacatcatcg ggggcgggag agaaatgaac
     1921 tggatccaga tggtggtcta ctgcgccggt ttttccacgg gaaatttcat cggctccctg
     1981 ctggaagaaa aactgatgaa cgcctatgtc ttcctggaac tcataatgga ccgaacggcc
     2041 gagacgaaag agatgatcga caatatccgt gcggacggtt tcggggcgac ggtgatctac
     2101 ggccgaggta gggacggcgt ccgcaccatc gtgaagataa tctgccgcag aagggacgta
     2161 cctgtcatcg tcgcgcacac acataataaa gggttcatct gcataagcga cgtcaagggg
     2221 tgctccggcg ggcagttccg tttccaacgc aagtaacttt ggttcatacc cctggatcac
     2281 ggaaggaagg gatggggctt catgaaggac agtctgggag ttgttcgcga gaggaacgtc
     2341 ggagagatcg gggggcgtca agcgggaaca tgtccggagg ggtactgcga aaagaccctt
     2401 cggctggaaa aagagttgac tcgctggaaa cgcgaggagg ccgttctgcg cgacattgaa
     2461 cggcgctatc ttgcgctgct tgacagcccg ctcctgatcc tgctgatcct ctccgagggg
     2521 agagtgctct ttatgaacag gcgcggcgag gagtttttcg gatttgcgct tcgcgaacgc
     2581 ccccgatttc tcctttcgga gtatgcagcg tccggatatg cttcatcggt agagagcctc
     2641 ttcactccgg gttccgacgg cgccgttcgc ggagggcgtc tcgacttcgc cgttcgttcg
     2701 gagagcggac gggag
//
LOCUS       DAOSWW010000305         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28512_length_2712_cov_4.191193, whole genome shotgun sequence.
ACCESSION   DAOSWW010000305 DAOSWW010000000
VERSION     DAOSWW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28512_length_2712_cov_4.191193"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK442_06910"
     CDS             complement(<1..423)
                     /locus_tag="PK442_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO87414.1"
                     /translation="MTRYDFDTVIDRRNTDCAKWDGMKPLFGTNDLLPMWVADMDFKA
                     PPEVIEALRERVEHGIFGYTRRFEPYREAIAGWFLRRHGWEIRTEWIRHAPGVVPALA
                     AAIMAFTQPGEGILVQPPVYYPFFSTIKGRGRTVVENPL"
     gene            833..>2712
                     /locus_tag="PK442_06915"
     CDS             833..>2712
                     /locus_tag="PK442_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOO87415.1"
                     /translation="MSVSYKERFRSSFSSSAPAGGERLSENALWLLDQRYFARRYDPV
                     LRGIRKEKSFEEFARRVARTVACAETSYLAGGEEDLQWLRRLERNIFDDILNRRFLFN
                     SPCLFGAGAGLTVDPGMAERIYCDPEELTFEDYSLLHRSKTKGQQLFACFVIEVPDSI
                     DGIFDSVKDAAVISKYGGGVGGNFGHLRERSSVINGGIGGQASGPVSFMETWNTMGAV
                     VVQGGRRRAALMGMLFDDHPDIFDFIDAKVDEGKLPYFNISVCVSDKLMKSAQTGADF
                     NLVSRSNGSVVRSLNGAELWSKLCENAWKRGDPGIFFIDRANADNLLKLDETWRIEST
                     NPCGEQPLPNYTSCNLGSVNVEAFVERREDGSVRFDFSAFSDQVFRSMYYLDLVIDAC
                     SYPLEKIGERTRRIRPVGLGIMGLADAAILLRMRYGSERFKVFCRTLADSMGSAALCA
                     SVGMAEEMGKEPFPDAHLVEELFRRFAAEQGEELFPESWFASLDRESMQSLVEKMRSS
                     PTIPYTLANAFESFFNAEGLGNEEERLALAAKVLQAFARGRLRTSRRLSIAPTGSISM
                     LIDASAGIEPNFAWSWSRRIAKADGDGQETREFHHKLLSPLQAAELHDTGALSDPVFV
                     TA"
BASE COUNT          559 a          750 c          791 g          612 t
ORIGIN      
        1 cagggggttc tctacgacgg tgcgccccct acccttgatg gtggagaaga aggggtagta
       61 gacgggaggc tgcaccagta ttccttcccc gggttgtgta aaggccatta tggcggccgc
      121 gagcgccgga acgactccgg gggcgtgacg aatccactcg gtgcgtattt cccagccgtg
      181 cctgcgaagg aaccatcctg cgatcgcctc gcgatacggt tcaaaacggc gggtgtagcc
      241 gaaaataccg tgttctacac gctcccggag ggcttcgatt acctcggggg gcgctttaaa
      301 atccatatcc gccacccaca tcggaagcag atcgtttgtc ccgaagagcg gtttcatgcc
      361 gtcccacttg gcgcagtcag tatttcttct gtcgatcacc gtatcgaagt cgtagcgcgt
      421 cataatgcga acctcgcttt caacaagttg ctactatcat atcatttttt taaacgagga
      481 gaagatgctg cattgagaaa aactgctttt ccttccccca aaagcgaaca attccagagt
      541 tatactaaaa atgcaaagct ttacgattta cgcccaggga aatagtctca cggagtcttc
      601 atttggaacg aattcataac cttgatggaa agttcataac aaaatgtgtg caaaactgct
      661 tctacaaaga gagggcttag gcgccatgtt cttccaattc tgaagtcacg cgtcccctct
      721 tgtaggaaag tggaagaagt ggtagtctga tagtccaatc gcaatttcac tcaacatctt
      781 gtgtgtgaaa cttttgtcat tgtatatata gtgtttttta ggaggggttt acgtgtcagt
      841 ttcctacaag gaacgctttc gttcttcctt ttcatcctct gcgccggcag gcggtgaacg
      901 tctctccgag aatgctctgt ggcttctcga ccagcgctat tttgcccgcc ggtatgaccc
      961 ggttctacgt ggaatccgga aggagaaatc gtttgaggag tttgcccgtc gggtagcgcg
     1021 tactgtcgca tgcgcggaga cgtcgtatct cgcaggaggg gaagaggatc tgcagtggct
     1081 tcgcaggctg gaacggaata ttttcgacga tatcctgaac cgccgcttcc tgttcaactc
     1141 cccgtgtctc ttcggagcgg gggcgggttt gaccgtggac cccgggatgg cggagaggat
     1201 ctattgcgat cctgaggaac tgacgtttga agactactct ttgctccatc gttcgaagac
     1261 gaaggggcag cagctcttcg cctgctttgt catagaggtc cccgacagta tcgacggcat
     1321 tttcgactcc gtcaaggacg cggccgtgat cagcaagtac ggcggcggcg tcggaggaaa
     1381 cttcggccat cttcgcgagc gcagctccgt catcaacgga ggcatcggcg gccaggcgag
     1441 cggtccggtg tcgttcatgg aaacgtggaa cacaatgggc gcggtggtgg tgcagggagg
     1501 ccgcaggcgc gccgcgctca tggggatgct cttcgacgac caccccgaca tcttcgactt
     1561 catcgacgcg aaggtcgacg aggggaagct tccgtacttc aacatctccg tctgcgtctc
     1621 cgataaactg atgaagtccg cgcagaccgg cgccgacttc aacctcgttt cccgaagcaa
     1681 cgggagtgtt gtccgctcgc tcaacggcgc cgaactgtgg agcaaactct gcgaaaacgc
     1741 gtggaagcgc ggcgatccag gcatcttttt catcgatagg gcgaacgccg acaatctgct
     1801 gaagctcgac gaaacgtggc gtatcgagtc tacaaatcca tgcggtgaac agccgctgcc
     1861 aaattatacc agctgtaatc tcggctcggt gaatgtagag gccttcgtgg agcgcaggga
     1921 ggacgggagc gttcgtttcg acttctccgc tttttcagat caggttttcc ggtcgatgta
     1981 ctatctcgac ctcgttatcg acgcctgctc ctacccgctg gagaagatcg gggagcgcac
     2041 gcggcgaatc cgtcccgtgg gactgggtat catgggactg gccgacgccg ccatcttgct
     2101 gaggatgcgg tacggctcgg agcgtttcaa ggttttctgc aggacgctcg ccgattccat
     2161 ggggagcgcg gcgctctgcg caagcgtcgg gatggccgag gagatgggga aggagccgtt
     2221 ccccgacgcg caccttgtcg aggagctctt ccgcagattt gcagctgaac agggggagga
     2281 gctcttcccc gaatcgtggt ttgcttccct tgatcgtgaa tcaatgcagt cgcttgttga
     2341 gaagatgcgt tcgtcgccga cgattcccta cacccttgcg aatgcgttcg agtccttctt
     2401 caacgccgag gggctcggca atgaagaaga gcgccttgcg cttgccgcga aggtccttca
     2461 ggcgttcgcg agagggcgcc tccgcacaag cagacgtctc tccatcgcgc ccaccggctc
     2521 gatctccatg ctcatcgacg ccagcgctgg tatcgaaccg aactttgcct ggagctggag
     2581 ccgccgtatc gccaaagccg acggcgacgg tcaggagacg agagagttcc atcacaagct
     2641 gctctcgcct cttcaggctg cggagctgca cgacacgggg gcgctctcgg accccgtctt
     2701 cgtgaccgcc ta
//
LOCUS       DAOSWW010000306         2710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28545_length_2710_cov_3.285499, whole genome shotgun sequence.
ACCESSION   DAOSWW010000306 DAOSWW010000000
VERSION     DAOSWW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28545_length_2710_cov_3.285499"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PK442_06920"
     CDS             <1..323
                     /locus_tag="PK442_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HOO87416.1"
                     /translation="QRVKVRIDFLDPVPPGYRLGDRFRIQVRIILRQNDDVLQIPLGA
                     LFRRGGDWVAFAVEEGRAVARKLEIGQNDGIYAEVLSGLSAGDRVILHPSEHVSEGAE
                     VTWK"
     gene            403..1119
                     /locus_tag="PK442_06925"
     CDS             403..1119
                     /locus_tag="PK442_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO87417.1"
                     /translation="MKILALNGSPRGAAGNTERMVQPFLEGARSAGAETEVLYVKDLK
                     IGFCKGCFACWTATPGRCVQRDDMDMVLEKMDAADTLLWATPLYHFGMTAMLKAVMER
                     TLPLADPHIVKAGDVYTHPGRSGKRFPQYLLFSNCGFPDQGHFEGLRAHFESLARASG
                     EHGTIPAVFRAAGELLRQKEVRPHIQWYFDALRQAGKEYVEHGGISGETMEVLHRDLV
                     PQEMYISQANAHWDSVLKGK"
     gene            1210..1797
                     /locus_tag="PK442_06930"
     CDS             1210..1797
                     /locus_tag="PK442_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87418.1"
                     /translation="MNPLGMIISFAPWLVLKILTSIPFSDPLTRLKTALVIAILICIY
                     QSRSAVKGFIFWGTIAFFSFSFVTVVLMTNMWVIDRLGLLSQLTMSAIAWGSILFKRP
                     FTLDYSKQYVPSEFWTHPAFLRKNYLITGVWGCYFLLGVAINEIRIYEPQISHLFLEV
                     LDNASMLAIILYTSHASKRRSVSASSENGQDTPVE"
     gene            complement(1987..>2710)
                     /locus_tag="PK442_06935"
     CDS             complement(1987..>2710)
                     /locus_tag="PK442_06935"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO87419.1"
                     /translation="PLAPSGRMLGTYTIFQDISLQAANEKRIGSIIRELNRTVAALKK
                     SWEQTVLVLAAITEVRDPYTSGHQRRVACLAAAIAGELGMDRKKVRQVELAAMIHDIG
                     KIEIPSEILSKPGKLSDIEYRLIQTHPAAGHRILQTIEVPWALAEIIHQHHERMDGSG
                     YPRGLSGSDILMEARIIAVADTVEAMSSHRPYRAGKGIEAALGEVASQRGKGFAPDVA
                     DACLRLFREKEFRFDEEPGRIG"
BASE COUNT          522 a          776 c          803 g          609 t
ORIGIN      
        1 agcagcgggt gaaggtccgg atcgacttcc tcgatcccgt gccgccgggg tatcgcctcg
       61 gagaccggtt ccggattcag gtgcgcatca ttctgcgcca gaatgacgat gtgctgcaaa
      121 taccgctggg cgcgcttttc cggcgcggcg gcgactgggt cgctttcgcc gtggaggagg
      181 gaagggccgt cgctcggaag ctggagatcg gccagaacga cggtatctac gccgaagtgc
      241 tctccggtct gtcggcgggg gacagggtga ttctgcaccc atcggagcac gtttcggagg
      301 gcgccgaggt gacatggaag tagcggcggc gaagaaattg ggatacatac cggcgcgtat
      361 cctccgcgcc ggaagagaaa actgaagaag gagcgtgaaa atatgaagat tctggccttg
      421 aacggcagcc cgagaggcgc tgctgggaac acggaacgga tggtgcagcc gtttctggag
      481 ggggcccgct cggcgggcgc ggagacggag gttctctacg tcaaggattt gaagataggc
      541 ttctgtaaag ggtgtttcgc ctgctggacg gcgactccgg gcagatgcgt ccagcgcgac
      601 gacatggata tggtgctcga gaagatggat gccgcggata cgctcctctg ggcgacgccg
      661 ctctaccact tcggcatgac cgccatgttg aaggccgtca tggagcgcac gctccccctc
      721 gccgatccgc atatcgtcaa ggccggcgat gtgtacaccc accccggccg ctccgggaag
      781 cgctttccgc agtacctcct tttttccaac tgcggcttcc ccgaccaggg gcatttcgaa
      841 ggtctcaggg ctcattttga atcgctcgcc agggcgagcg gagagcacgg gacaattccg
      901 gcggtctttc gcgcggcagg agagctctta cggcagaagg aggtccggcc gcacatacag
      961 tggtacttcg acgcgctgcg ccaagcggga aaagagtacg tcgagcacgg cggcatctcc
     1021 ggcgaaacaa tggaggtatt gcaccgggat ctcgtcccgc aggagatgta catttcgcag
     1081 gcgaacgcgc actgggactc ggtgttgaag gggaaataag aaggcgactg tgctattatt
     1141 accctgccgt aagcgagcgt accgcctttc gtccgtcggc cctacactat tttcttggga
     1201 ggttgtactg tgaatccttt gggtatgatt atttccttcg ctccgtggct tgtgctcaag
     1261 atcttgacca gcattccttt ttccgatccg ctgaccaggc tgaaaacggc gcttgtcatc
     1321 gcgattttga tatgcatcta ccagtcgcga agcgcggtga agggctttat tttctgggga
     1381 accatcgcgt tcttctcctt cagcttcgtc accgtagtgc tgatgacgaa catgtgggtc
     1441 atcgatcgcc tcggtctgct gtcgcagctg accatgtccg ccatcgcctg gggatcgatc
     1501 ctctttaaac ggccctttac gctcgactac tccaaacagt acgtcccgtc cgagttctgg
     1561 acgcaccccg cctttctccg gaagaactac ctgatcacgg gcgtatgggg gtgctacttc
     1621 cttctgggcg tcgcgatcaa cgagatacgg atctacgagc cgcagataag ccacctgttt
     1681 ctcgaagtgc tcgacaacgc ctcgatgctc gccatcatcc tctatacgtc gcacgcttcg
     1741 aagcggcgca gcgtttcagc ttcgtcggaa aacggacagg atactccggt agaataatga
     1801 ttgtgtgtta tgctgtttga ggaaccattt ttattaaagt ctcgtaaaat atcccttttg
     1861 tcggaataca ctccgagcgt aagaagagtt tagatgatga tgaaacggcg gacaatttgt
     1921 ccgccgtttt ggtttcgcta ttcgccgtcg taacgcggtc cgggaggagt ggtaagtttt
     1981 tccgtctcag ccgattctcc ccggttcctc gtcgaaacgg aattctttct cccggaaaag
     2041 acgcagacaa gcgtccgcga catcgggggc gaaccctttc cctctttgcg aggccacctc
     2101 tcccagggcg gcttctattc ctttccccgc cctgtaggga cggtgcgacg acatggcctc
     2161 caccgtatct gcgaccgcga tgatgcgcgc ctccatcaga atgtcggagc cgctgagccc
     2221 gcgggggtac cccgaaccgt ccattcgctc atggtgttga tggatgatct ccgccagcgc
     2281 ccatgggacc tcgatcgtct gaagaatcct atgcccggca gccgggtggg tctgaatcag
     2341 ccggtattcg atatcgctca atttgcctgg tttcgagagt atctccgagg ggatctcaat
     2401 cttcccgatg tcgtgaatca ttgccgcaag ctcaacctgc cgtactttct tcctgtccat
     2461 gcccagttcg ccggcgatag ccgccgccag acacgccacc ctgcgctgat gtccggacgt
     2521 gtacggatcg cgcacctccg ttattgctgc cagaacgagg acggtctgct cccatgattt
     2581 tttgagcgcg gcgacggttc tgttgagctc ccggatgatg gagccaatac gcttttcgtt
     2641 cgccgcctgt agagaaatgt cttggaagat cgtgtacgta ccgagcatcc tccccgaagg
     2701 agccagaggg
//
LOCUS       DAOSWW010000307         2700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28709_length_2700_cov_3.756144, whole genome shotgun sequence.
ACCESSION   DAOSWW010000307 DAOSWW010000000
VERSION     DAOSWW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28709_length_2700_cov_3.756144"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PK442_06940"
     CDS             <1..1217
                     /locus_tag="PK442_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOO87420.1"
                     /translation="AVLPENAVEAALRGEAFSARAQSGGKIVLAAIGKAAWRMAKAAA
                     ETLGDRLDGGVVVTKYGHDNGSIKGVEIFEAGHPFPDENTIKGTARALERVGGLSAED
                     TVLFLVSGGGSALFEKPGEGITLRDLVGVTDQLLACGADIVEINTIRKRLSSVKGGRF
                     AQFVAPAHVFSVVLSDVLGDRLDSIASGPAYPDGSTAADALRIVGKYGLSLSPVLQKA
                     LEKETPKSLENVTTLITGSVTALCKAAEREVAALGYAPLLLTTTLNCEAREAGAFIAA
                     MAKEIRSSGNPASPPCALLLGGETIVRLKGKGKGGRNQEMALAAAFGIKGLADTAVIS
                     VGSDGTDGPTDAAGGVVDGGTIGALSGLGLDAEALLADNDSYHALDACGGLVKTGPTG
                     TNVNDLSILLCR"
     gene            complement(1297..2556)
                     /locus_tag="PK442_06945"
     CDS             complement(1297..2556)
                     /locus_tag="PK442_06945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO87421.1"
                     /translation="MERCGGVHFEKVVNKSPVTSFIWLPSENGKPCFVSEAIRSFGYA
                     PEDFTSGKIRYADLLHPDDVAAFSFSPGSEPQIYRISDSEGAWRWVFHRSCLIGEEAG
                     YPEELLWNISDITYYKEADEELRKNESKLKALMKAIPAILLVIDEDGRYIDVSGSSEA
                     ALVTASSELIGKTVYESFPNEKAEELMAPLKRCVETGAPQFAAYDIDIGGKKYFQEAR
                     ISLLPELVEGKRTVICVALDVTAQKELEEEILLQTGHNPIDGSSNRTHFNRAFLFSLA
                     EAQRTGGTLAFFMIDLDHFNTVNETIGREMADLLLKGIGQRLQKVCRQDDILYRMEGV
                     NYYLILPKPGTREDMGAVAERFLETIRGASSGYTGSFKLSATIGISVFPEDGQDAKTL
                     LRTAEAALSAGKAEGGNCYRFAEKPAR"
BASE COUNT          495 a          784 c          858 g          563 t
ORIGIN      
        1 aggccgtgct ccctgaaaac gccgtggagg cggcgctgcg cggagaggcg ttttcagcac
       61 gtgcgcagtc cggagggaag atcgtgctcg cggccatcgg caaagcggcg tggcggatgg
      121 cgaaggccgc cgctgaaacg ctgggagatc ggctcgacgg cggtgtcgtc gtgacgaagt
      181 acggacacga caacggctcg ataaagggcg tcgagatttt cgaagcgggg cacccgtttc
      241 cggacgagaa cacgatcaag ggcacggccc gtgcgctcga aagggtcggc ggactctccg
      301 cggaggatac tgtgctcttc ctcgtctccg gcggcggctc ggccctcttc gagaagccgg
      361 gcgaggggat taccctccgg gatctcgtcg gagtgaccga ccagctgctc gcatgcggcg
      421 ccgacatcgt cgagatcaac acgatacgaa agcggctctc ctcggtcaag gggggacggt
      481 tcgcccagtt cgtcgctccg gcgcacgtct tttcggtggt gctctccgac gttctcggcg
      541 acagactcga cagcatcgcc tccggcccgg cctatccgga cggttcgacg gccgccgacg
      601 cgttgcggat tgtcgggaag tacgggctgt cgttgagccc ggtacttcag aaagctttgg
      661 agaaggagac cccgaaatcg cttgaaaacg tgaccacgct cattaccggg agcgtgacgg
      721 cgctctgcaa agcagcggag cgcgaggtcg ccgcactcgg ctatgcgccg ctcctgctga
      781 cgacgacgct gaactgcgag gcccgcgaag cgggggcctt catcgccgct atggcgaagg
      841 agatccggtc ttccggaaac ccggcgtcgc ctccgtgcgc cctgctcctc ggcggggaga
      901 cgatcgtccg gctcaaaggg aagggaaagg gcggacggaa ccaggagatg gctctggccg
      961 cggccttcgg catcaagggg cttgcggata cggcggtgat ctccgtcggt tccgacggca
     1021 cggacgggcc gacggacgcg gcgggtggcg tggtggacgg cgggacgatc ggcgcgcttt
     1081 ccggtctcgg acttgatgcc gaagctctgc ttgccgacaa cgactcctat catgcgctcg
     1141 atgcctgcgg aggccttgtc aaaacggggc ccaccggaac gaacgtgaac gacctctcca
     1201 ttctcctctg caggtagtcg ctctttcggg tgcagacaga acgaggcccc tgaaaagggg
     1261 cctcgcgtca tattcatgtg cagtggcgga gaaaaattac ctggcgggtt tctcagcgaa
     1321 tctgtagcag ttgccgccct ccgcttttcc tgcggaaaga gccgcttccg ccgttctgag
     1381 cagcgttttc gcgtcctgcc cgtcttccgg gaagacgctg atgccaatgg tggcggaaag
     1441 cttgaatgag ccggtgtatc ccgaggacgc tcccctgatt gtttccagga agcgctccgc
     1501 aacggccccc atgtcttccc tggttcccgg cttcggcagt atgaggtagt agttgacgcc
     1561 ctccatgcgg tagagaatgt cgtcctgccg gcacaccttc tgaagccgct gccctatacc
     1621 cttgagtaga aggtcggcca tctctctccc gatggtctcg tttacagtgt tgaaatggtc
     1681 gagatcgatc atgaagaaag ccagcgttcc gcccgttcgc tgcgcctcgg cgagggagaa
     1741 aaggaacgcc ctgttgaaat gcgtcctgtt cgagcttccg tcgatcgggt tgtgtccggt
     1801 ttggaggagg atctcctcct caagctcttt ctgcgcggtg acgtcgaggg cgacgcagat
     1861 cacggtccgt ttgccctcca caagctccgg caggagggag atgcgggctt cctgaaaata
     1921 ttttttcccg ccgatgtcga tgtcataggc tgcgaactgc ggggcgccgg tctcgacgca
     1981 gcgtttcagc ggcgccatca gctcctccgc tttttcgttc gggaacgatt cgtagacggt
     2041 tttcccgatc aactccgagg acgctgtgac gagggcggct tcggaggaac ccgagacgtc
     2101 gatgtaccgt ccgtcttcat ctatgacaag gagaatcgcc gggattgcct tcataagcgc
     2161 cttgagcttc gattcatttt tccgcagctc ttcgtcggcc tctttgtaat aggtaatatc
     2221 cgagatattc cacaagagct cttccgggta gcccgcttct tcgccgatga gacacgaacg
     2281 gtgaaagacc cagcgccacg ccccttccga gtcggagatc ctgtagattt ggggttcgga
     2341 gccgggcgag aatgagaaag cggcgacatc gtccgggtga agaagatcgg cgtatctgat
     2401 tttcccggat gtgaagtcct ccggcgcgta gccgaacgag cgtatggctt ccgatacaaa
     2461 acacggtttc ccgttttcgg aggggagcca gatgaaagaa gtcacgggac tcttgttgac
     2521 aaccttttcg aagtgtactc cgccgcatcg ctccatgccg gctttcatct ccctttagag
     2581 gtatgtcccg gagaatccgg aatgtttttc ttttcctcaa ggtattgtag catatatgaa
     2641 cctgccgaaa cagtttttcc cggatgtgcg gcgttttcaa cgggatgtgt tgttgggggc
//
LOCUS       DAOSWW010000308         2699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28725_length_2699_cov_3.209531, whole genome shotgun sequence.
ACCESSION   DAOSWW010000308 DAOSWW010000000
VERSION     DAOSWW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28725_length_2699_cov_3.209531"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PK442_06950"
     CDS             complement(<1..498)
                     /locus_tag="PK442_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOO87422.1"
                     /translation="MRRSGECSRTYCVFPGLLLLLGGAALWRLLSGDMAIPLSGALKA
                     VLGLEGVLTPQQALVIQAVRLPRLLASLGAGASLAVSGVVLQGILVNPLAEPYTLGIA
                     SGAALGASLGISLGGAWIPAAAFAGALAALFLSLLLAWRSGGFSPLHMVLSGIVIGSV
                     LSAGVT"
     gene            complement(568..1461)
                     /locus_tag="PK442_06955"
     CDS             complement(568..1461)
                     /locus_tag="PK442_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HOO87423.1"
                     /translation="MRKMSCAVFVFVVCLFGLLSASAFASEHAKPEGKQGILLVAFGT
                     SEPDARSAIHGIVDAVRKAFPDAEVRLSYTSNIIRRKILKEEGLVVDTPPIALAKMQD
                     EGFTSVVVQSLHIISGEEFHQLVGIVRAFSSIRGKYGFERLSLGRPLLDSMEDYRKTV
                     DMLKAKYASFAADGGVVTFMGHGTHHAANAAYSKMQLLFDEEGLPFVMGLVEAFPDIE
                     SVKRRLKELKPSRVTLVPFMVVAGDHAKNDMADTEDPESWVRVLASEGYAVEPLLKGL
                     GDGEGLAELFIGHIREALEDR"
     regulatory      complement(1685..1895)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2005..2478
                     /locus_tag="PK442_06960"
     CDS             2005..2478
                     /locus_tag="PK442_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOO87424.1"
                     /translation="MTEKSFNMKDALRYGIHAEIEAKNFYHTWAESVQEEHLKKELSA
                     LSDWESEHEASLMNYYEKLFGAQCPIDPNMVVEPELHVQTKDFGDTTSLLRVASAAYL
                     SEMRAMEFYERLEKESTGETAAMFRKIKDMEKEHMDITKKRYMKIREDVVGFRAF"
BASE COUNT          613 a          855 c          767 g          464 t
ORIGIN      
        1 cgtcaccccg gcggagagca ccgagccgat cacgatgcca gagagcacca tgtgcagcgg
       61 agagaatcct ccagaacgcc atgcaaggag gagcgaaaga aaaagagctg caagcgcccc
      121 ggcgaaggcg gcagctggaa tccatgcgcc gccgagggag atccccagcg aagcgccgag
      181 cgccgctccc gaggcgatgc cgagcgtgta cggctccgcc agcgggttca ccagaatccc
      241 ctggagcacc acccccgaca cagcgagcga ggcgcccgct cccagcgacg cgagaagccg
      301 cggcagccgg accgcctgta tgacgagcgc ctgctgcggg gtaagaacgc cttccaatcc
      361 aagaacagcc ttgagcgcgc cggaaagggg tattgccatg tctccggaga gcagacgcca
      421 cagggccgct cctcccagaa ggaggagcag ccccgggaaa acacagtacg tccgcgaaca
      481 ttcaccggaa cgtcgcatac tgaatagccg ctcctccggg gcggcgctcc cggacaaatc
      541 cggagagccc tcggccgcgc ttcgttctca cctgtcctcc agagcctcgc gtatgtggcc
      601 gataaagagc tccgcaagcc cttcgccgtc tccgagcccc ttcaaaagag gctcgacggc
      661 atacccttcg ctcgcgagca ctctgaccca ggactccggg tcctctgtat ccgccatatc
      721 gttcttcgcg tgatccccgg ccaccaccat gaagggaacg agcgttaccc gcgaaggttt
      781 cagctccttc agccgccgtt tcacgctctc gatgtccggg aaggcctcga cgagtcccat
      841 gacgaaaggc agcccctcct cgtcgaagag gagctgcatc ttgctgtacg ccgcattcgc
      901 cgcgtggtgc gtaccgtgcc ccatgaaggt cactacgccg ccgtcggccg caaaggaggc
      961 gtacttcgcc ttcagcatgt ccaccgtctt tctgtaatcc tccatgctgt cgagcagcgg
     1021 tctgccgagg gaaagacgtt cgaatccgta cttcccgcgt atggacgaga aggcgcgcac
     1081 tatcccgaca agctgatgga actcctcgcc cgagatgata tgcagcgact gcacgacgac
     1141 cgacgtaaag ccttcgtcct gcatcttcgc cagcgctatc ggaggcgtgt cgacgacgag
     1201 cccctcctcc ttgagtatct tccggcggat gatgttcgaa gtgtaggaaa gccggacctc
     1261 ggcgtccgga aaggccttcc gcaccgcatc gacgatgccg tggatggccg aacgggcgtc
     1321 gggctccgac gtcccgaagg cgacaagcag tattccctgt ttcccctcgg gcttcgcgtg
     1381 ctccgaagcg aacgcgcttg ccgaaagcag gccgaacaga cacacaacaa acacaaagac
     1441 cgcacaactc atttttctca tcctttaccc ctcccgtgga gtgatagccg ggagaatgct
     1501 ccccggacgc catgggctcc cggaggaaac gagcgcacac cgccgtgcag ttggcgggga
     1561 taatacaaag gaccgttcca cacaggaaac ggcccctccc agtgatcttc ttccaacgcc
     1621 gctccccgtc gagtctccgg ggaatgcgtg ggaaacactt cggcggcctc gattcctcgc
     1681 gagtcttccc gcagtttcga aagaccggtc tcctggctcg ccttcatcct cccccacccc
     1741 ttcccggaac ccttcgtacg cagtgctgcg ccggttcgct ccagtggctc ggtggcttcg
     1801 tcaggcttac agtggcgggt ccgcgccgga ttttcaccgg acttcccgaa catcttccga
     1861 aagaaggcgc acgcgccttc gttctgatag tagtccgacg ggaagggttt gtcaaggcga
     1921 ggaaaagctc ctgcgttttt ggaatttttt cgggaatggg gtataataca ggaaagaatc
     1981 taatcttgca caggagggat gacgatgaca gaaaagagtt ttaacatgaa ggatgcgctc
     2041 cgctacggga tccacgctga aatcgaggcg aagaactttt accacacgtg ggcggagagc
     2101 gtccaggaag agcacttgaa gaaagagctg tcggccctct ccgattggga gagcgaacac
     2161 gaggcaagtc tgatgaacta ctacgaaaag ctcttcggag cgcagtgccc catcgacccg
     2221 aacatggtcg tggaaccgga gctgcacgtt cagacgaagg acttcggcga cacgacatcg
     2281 ctgctccgcg tcgcctccgc cgcatatctc tccgagatgc gcgcgatgga gttttacgag
     2341 cgcctcgaaa aggagtccac gggcgaaact gccgccatgt tccgcaaaat caaggacatg
     2401 gaaaaggagc acatggacat cacgaagaaa cggtacatga aaatccgcga ggacgttgtc
     2461 ggcttccggg cattctgaag ccgtactccc gtaaggaaaa catcccgata aagagacgga
     2521 ctccgggaag agcgcggagt ccgtctcttt ggtccagcag ctgaagaaaa cgctcaaaag
     2581 cctctattgc atcatcctcg gcaggaagag aatgatatcg gggaagaagt tcaagacgac
     2641 tactgtagct atcagcgccg caaccaaggg aacaatctcc ttcaccaacg actccaact
//
LOCUS       DAOSWW010000309         2699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28735_length_2699_cov_2.083964, whole genome shotgun sequence.
ACCESSION   DAOSWW010000309 DAOSWW010000000
VERSION     DAOSWW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28735_length_2699_cov_2.083964"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2699
                     /locus_tag="PK442_06965"
     CDS             <1..>2699
                     /locus_tag="PK442_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO87425.1"
                     /translation="YYFALATDPAKVRRMVASVPSGEDRPELFFSDDLKADRLARSLP
                     AVHCRDCGAMGWASSEEKGGSVEIRDLKAFYSAFFSYSPKIRYLFPGEGGAEHRGLAG
                     RTRKLCSSCMALSDVRDGEDCPFCGNSPLLPVFVPDSQFKKTYENGDNRVFSHNDCPF
                     CGSSRGLSIFGARSTTLSSVMISQTMASRGNDDKKMLAFSDSVQDAAHHAGFFGARTY
                     RFTLRTAMAKYLADGGEGKTLSGFAGELLRYWAFRLSMEECVALFLAPDQAWRRDFAR
                     LKETGTPPGAEFMELLSKRLGWEAFTEFGLSAHIGRTLEKSGVAVASPGPALFGHSAA
                     LLAEALKNELPKMTPPEEREISFFLSGLIQRMKSKGAIHHPELEEYIRRGGEGYLLSK
                     ERKGWMPSYGKRTRRPLFLSTAADGDFDRLSFSESRNWYCRWMMKCFGENNPMMLSSA
                     ADFWRTVLGVLSSEPSGILRRLSWPRGRGSEEPVWALEPSALRMTCGVAEFRCSTCGA
                     SSFAGADGAEDWNGLPCLRDSCDGRLAESSISGEYYGRLYRTGDVCRLFPAEHTGMLP
                     KGRRALLEAAFKQREGRKPWDPNLLSCTPTLEMGIDIGDLSTLMLCSVPPDTASFRQR
                     VGRAGRRDGNSLAFTVANGRPHDLYFFGEPLEMIVGDVEPPGVFLGASAVLERQFAAF
                     CIDCWVRSGITAKDLPEKTSTVLNQLDSSEKNCFPHSFLKFIDRERSPLLDRFFALFG
                     GEIADDVKRHIEKYASSPEDGLTFSLLEGLRRMQKQRASLQHKAKALRKALGKLKEDP
                     AAKPEEVEALSREKRALERLFRDLGELPLFNLLTDEGLIPNYAFPESGVVLRSVIYRK
                     LEDGRLESDIFKYERAAGAALRELAPSSTFYAGGRKVKIEQVD"
BASE COUNT          463 a          821 c          916 g          499 t
ORIGIN      
        1 cctactattt cgccctcgcc accgatccgg cgaaggtgcg gcggatggtg gcgtcggttc
       61 cctcggggga ggatcgcccg gaactcttct tctccgacga tctgaaggcg gaccggctcg
      121 cccgctccct tcccgcggtt cactgcaggg actgcggggc gatgggatgg gcctcgtccg
      181 aggaaaaggg cggttccgtg gagatacggg atctcaaggc gttctacagc gcctttttca
      241 gctacagccc gaagatccgc taccttttcc ccggcgaggg aggggccgag catcgggggc
      301 ttgctggaag gacgagaaag ctctgttcat cgtgcatggc gctttccgac gtgcgggacg
      361 gagaggactg ccccttctgc ggaaactccc ctctgctgcc cgtcttcgtt cccgactcac
      421 agttcaagaa gacctacgag aacggcgaca acagggtatt ctcacataac gactgccctt
      481 tctgcggttc gtcaaggggg ctctccatct tcggcgcccg gagcacgacc ctgtcgagcg
      541 tcatgatctc gcagaccatg gcgtcgcggg gaaacgacga caagaagatg ctggccttct
      601 ccgactcggt gcaggatgcc gcccatcacg ccgggttctt cggggcgagg acctacagat
      661 tcacgctgcg gaccgccatg gcgaagtacc ttgccgacgg cggggagggg aagacgctct
      721 ccggtttcgc cggggagctg ctccgctact gggctttccg cctgtccatg gaggagtgcg
      781 tcgcgctctt tctcgcccct gaccaggcgt ggagaaggga tttcgcccgt ctgaaggaga
      841 ccgggacgcc gccgggcgcg gagttcatgg agttgctgtc gaagcggctc gggtgggagg
      901 cgttcaccga gttcggcctg agcgcccaca tagggcgcac cctggagaaa tcgggcgtgg
      961 ccgtcgcctc gcccggcccg gcgttgttcg gccattccgc cgccctcctc gcggaggcgc
     1021 tgaaaaacga actgccgaag atgacgccgc cggaggagcg ggagatctct ttttttctct
     1081 ccgggctgat ccagaggatg aagtcgaagg gggcgatcca tcaccccgag ctggaggagt
     1141 atatccggcg cggaggagag gggtacctgc tctcgaaaga gcggaaaggg tggatgccct
     1201 cctacgggaa gcggacgcgg cggcctcttt tcctctccac ggcggcggac ggcgatttcg
     1261 atcgcctctc cttttcggag tcgagaaact ggtactgccg gtggatgatg aaatgcttcg
     1321 gggagaacaa ccccatgatg ctctcgtcgg cggcggattt ctggcggacg gttctcggcg
     1381 ttctctcgtc cgaaccttcc ggcatcctga ggaggctttc ctggcccaga ggaaggggga
     1441 gcgaagagcc tgtatgggct cttgaacctt ccgcgctccg catgacttgc ggcgtggccg
     1501 agttccggtg ctcgacgtgc ggggcgtcct cttttgcggg ggcggacggc gcggaggact
     1561 ggaacgggct tccctgcctc cgggactcct gcgacggcag actcgccgaa agctctattt
     1621 cgggagagta ctacggaagg ctgtaccgca cgggagacgt gtgccgcctt tttcccgcgg
     1681 agcacaccgg tatgctgccc aaggggcggc gggctctgct ggaagctgcg ttcaagcagc
     1741 gggaggggcg gaagccgtgg gatccgaacc tcctttcgtg cacgccgacg ctggagatgg
     1801 gcatcgacat cggcgacctt tccacgctga tgctgtgttc cgttccgccc gatacggcct
     1861 cgttccgcca gcgggtgggc agggcgggaa gacgggacgg aaattccctc gccttcacgg
     1921 tagccaacgg ccgcccccac gatctctatt tcttcggcga gcccctcgag atgatcgtgg
     1981 gcgacgtgga gccgccggga gtcttcctgg gcgcgtccgc cgtgctcgag cgtcagttcg
     2041 cggcgttctg catcgattgc tgggtccgat cggggatcac ggcgaaggac ctccccgaaa
     2101 agacctcgac ggtgctcaac cagctcgaca gcagcgagaa gaactgcttc ccgcacagct
     2161 tcctgaagtt tatcgacagg gagcggtcgc cgctcctgga ccgcttcttc gcccttttcg
     2221 gaggggagat cgcggacgac gtgaaacgcc atatcgagaa gtacgcatcc tctccggaag
     2281 acgggcttac cttcagcctg ctggaggggc tccgcaggat gcagaagcag agggcctccc
     2341 tgcagcacaa ggcgaaggcg ctgcggaaag cgctggggaa gctgaaggag gaccctgccg
     2401 cgaagccgga ggaggtcgag gcgctgtcga gggagaaacg cgccctggaa cggctcttcc
     2461 gcgacctcgg cgagcttcct cttttcaacc tgctcacgga cgaggggctg ataccgaact
     2521 acgcctttcc cgagagcggg gtcgtgctcc gctccgttat ctacaggaag ctggaggacg
     2581 gcaggctcga gagcgatatt ttcaaatacg agcgcgcggc gggcgccgcc cttcgggagc
     2641 tggcgccttc gagcaccttt tacgccggag ggcgcaaggt gaagatcgag caggtggat
//
LOCUS       DAOSWW010000310         2698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28740_length_2698_cov_4.753689, whole genome shotgun sequence.
ACCESSION   DAOSWW010000310 DAOSWW010000000
VERSION     DAOSWW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28740_length_2698_cov_4.753689"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="PK442_06970"
     CDS             complement(<1..1798)
                     /locus_tag="PK442_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87426.1"
                     /translation="MYQYQPMQLPDFSQAGREMVSGLGALVMMQQARESERRASSEAA
                     LGRAERAADREAKLRAQMLEDARLRRQDDRQAFADLMTLRKYERESVVSDLQNIQVLQ
                     QMEKRKGQEQLELVHQGDISLALNAGSPAESALAIGRMASDGVLSGLRPELQQQLLTL
                     QSGQHSTPIPIAGEAAIPLSAIDGMLRSSDEDQKARALAVYFSQNGSEQALGSYGLSP
                     ATIAMGKRLAGITGNHEAAKQAGAIQKQFQNLSQQRALLLGYIQSSIGSTDPAVRGEA
                     EKAKEQLRALKPAMQSLQTLFENLPKTSGMSLDPNTGSIEFDSGVPAAMLVAEGFSDS
                     FRTKGVFAPFEQQLASLAEDAKAGKLDRDTFMKRAQQLKNELPNPQDFTVSMDRLFVL
                     ARAKSEGRLDPYSDEAVGLLSSTDWSKGIGQVETAVGGNPVAFVSRVLGKDFVEAAAN
                     YAAYKENVADFERKGELPGLTKKAPRVTRDQALQELRDLKTLEELLPEDEFSGVQRSR
                     LRFLEDALEMTLDPATGDYLHVSQVSAPGAFPEEARALHTEAYRSGITDAIFANPRYE
                     DELLGDATKEAKAVFRSATIVGSPRQQALTLAR"
     gene            complement(1809..2474)
                     /locus_tag="PK442_06975"
     CDS             complement(1809..2474)
                     /locus_tag="PK442_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87427.1"
                     /translation="MEGFGGQKQWEAIEQHGLTDASQIGVLPEDVKAQTDSLYGGTMD
                     ALSKMEDSINRMSEANASLLKGEIPADVSSAVRRAAAESSMAGGVFGSSARNLSARDL
                     GKTSFDIQQTGFANEQAIQSGYDALGKAKESIREYSLNREATLQELQVKARQVNMSGI
                     DLERSRIATNIDSNLKILDMMTNMIVNQQSIAASAAANDVDPGNIIASIDGWLTKFNE
                     KLA"
     gene            complement(2479..>2698)
                     /locus_tag="PK442_06980"
     CDS             complement(2479..>2698)
                     /locus_tag="PK442_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87428.1"
                     /translation="KPRPGLVYAGAQPGLRLWLLQFFSVFRRFRQQVFRRFRQQVFRR
                     RRGAGPGSHSQRGCSRFRRGSHFVTEGF"
BASE COUNT          483 a          858 c          791 g          566 t
ORIGIN      
        1 cgcgcgccaa agtgagtgcc tgctgccggg gggacccaac tatagtggcc gaacgaaaca
       61 ccgccttcgc ttccttcgtc gcatcgccaa gcagctcatc ttcatatcgg gggttagcga
      121 aaatagcgtc ggttattccg gaacggtacg cctccgtgtg gagggcgcgc gcctcctcag
      181 ggaacgcgcc gggtgcggaa acttgcgaaa cgtgcaggta gtctcctgtg gccgggtcta
      241 gcgtcatctc caacgcatcc tcgagaaacc gcagccggga gcgctgcact ccgctgaatt
      301 cgtcttcggg aagaagctcc tcaagcgttt tcagatccct aagttcctgt agcgcctgat
      361 cacgcgtcac gcggggggcc ttcttcgtga ggccgggaag ctctcccttc ctctcgaaat
      421 cggccacatt ctccttgtat gccgcgtagt tcgcggcggc ctcgacaaaa tccttgccaa
      481 gaacacgact tacgaaagca accggattcc ctcctacggc agtctccacc tgtccaatgc
      541 ccttcgacca gtcggtgctg ctgaggagac ccaccgcctc gtcactataa gggtcgagcc
      601 ggccctcaga cttggctctt gcgaggacaa acagacggtc catcgatacc gtaaagtctt
      661 gaggattcgg tagctcgttt ttaagttgtt gggcccgctt cataaaagtg tcgcgatcaa
      721 gcttccctgc cttcgcgtct tcggcgagcg aggcgagctg ctgctcgaac ggcgcgaaca
      781 cgcccttcgt gcgaaacgaa tcggagaacc cctccgcgac aagcatcgca gcaggaaccc
      841 cggagtcaaa ctcgattgag cctgtattcg ggtccagaga cataccggac gtctttggaa
      901 gattctcaaa caacgtctgc agactctgca tggctggctt taacgcgcgg agctgctcct
      961 tggccttctc tgcttctccg cgcaccgccg gatccgtaga accgatggag ctctggatgt
     1021 agccgaggag aagcgcccgc tgttgggaaa gattttggaa ttgcttctga attgccccgg
     1081 cctgcttcgc cgcctcatga tttcctgtga tcccggccag ccgcttgccc atagcgatgg
     1141 tggccgggga cagcccgtag gatccgagcg cctgctcgct gccattctgg ctgaagtaca
     1201 cggcgagagc ccgtgccttc tgatcttcat cggacgagcg gagcatcccg tcgatcgcgc
     1261 tcaacgggat cgcagcctcc ccggcgatcg gaatcggcgt gctgtgctgc ccgctctgga
     1321 gcgtgagcag ctgctgctgc agctcgggtc ggaggccaga caaaactcca tctgacgcca
     1381 ttcgtccgat ggccagcgcg gactccgccg gactcccggc gttcagagcc aggctgatgt
     1441 cgccttggtg cacaagttcc agctgctcct ggcctttccg cttctccatc tgttggagca
     1501 cttggatatt ctgaagatcg gacacgacgg actcgcgctc gtactttcgg agggtcatca
     1561 aatcggcgaa cgcctgccgg tcgtcctggc ggcggagccg ggcgtcttca agcatctgtg
     1621 cacgaagctt cgcctcccga tccgcggcgc gttcggcacg gccaagggcg gcctcgctgg
     1681 acgctcgccg ctcggactcc ctggcctgct gcatcatgac aagtgcgccg aggccggaga
     1741 ccatctctct cccggcctgg ctgaaatcgg gaagctgcat cggttggtac tggtacatgg
     1801 tcgatctcct acgcgagttt ttcgttgaac ttggtcagcc atccgtcaat agacgcgatg
     1861 atgtttccag ggtcaacgtc attggccgcg gcgcttgcgg cgatgctttg ctgattaaca
     1921 atcatgttcg tcatcatgtc aagtatcttc aaattcgaat cgatgttggt tgctatccgg
     1981 ctccgctcca aatcgatccc ggacatgttt acctgccgcg ccttgacctg cagctcctgc
     2041 agggtggcct cgcggttgag ggagtactcc cgaatgcttt ccttcgcttt ccccagggcg
     2101 tcgtacccgg actggatcgc ctgctcgttc gcgaaccccg tctgctggat gtcgaacgag
     2161 gtcttcccga ggtctcgggc ggacaggttc ctggcagagg atccgaatac cccgccggcc
     2221 atcgacgact cggccgcggc gcggcggact gcggagctga cgtctgcggg gatctctccc
     2281 ttcagcaacg aagcgttcgc ctccgacatg cgattgatcg agtcctccat tttggacagc
     2341 gcatccatgg ttccgccgta cagagagtcc gtctgggcct tcacgtcctc cgggagaacg
     2401 ccgatctggc tcgcatcggt cagcccgtgc tgttcaatgg cttcccactg cttttggccc
     2461 ccgaagcctt ccatgacatt agaagccttc tgtgacgaag tggctacctc ggcggaagcg
     2521 ggagcacccc cgttggctgt gcgagccggg gccggcgccg cggcgccgcc ggaagacctg
     2581 ctgccggaac cgacggaaga cctgctgccg gaaccgccgg aagaccgaga agaactggag
     2641 gagccagagc cgtaaacctg gttgagcccc tgcataaaca agtccgggtc gaggcttc
//
LOCUS       DAOSWW010000311         2694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28820_length_2694_cov_3.765063, whole genome shotgun sequence.
ACCESSION   DAOSWW010000311 DAOSWW010000000
VERSION     DAOSWW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28820_length_2694_cov_3.765063"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..935)
                     /locus_tag="PK442_06985"
     CDS             complement(192..935)
                     /locus_tag="PK442_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87429.1"
                     /translation="MLEHETVNKLKQMKLTGIAEALKEQNDDEGYREMGFHDRFSLLV
                     DREFCKRQHGRLQRLINGAKFENPTACIEDIRYDDDRKLDRNFILELASCNYVPHARN
                     IVIVGPTGCGKSFLSQALGHAACRRLLKTRYVQLPDLLDELKMARTKGMEAFDRLRKQ
                     FIKYDLLIIDEWLLFPISMEDTQILLSLVDRRHNHQATIIASQFEPAEWLDQIPVPVA
                     AEAITDRLCSQAYKIVIKGKKSMRETVRI"
     gene            complement(928..2481)
                     /gene="istA"
                     /locus_tag="PK442_06990"
     CDS             complement(928..2481)
                     /gene="istA"
                     /locus_tag="PK442_06990"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOO87430.1"
                     /translation="MSDFLTIVRAVYSGLSQRKVAATHGVSRNTVSLYLRKARDQGWL
                     TLKDLDAVDDNAVAQCLQQIAAPSRDATFKMPDFDYVHAELAKAHVTLKLLWEEYVEQ
                     CRQSNERFYMETQFRRYYHLHAKVHKATIRLEHKPALSLEVDWAGTRIAFFDAESGKM
                     SQASLFVAVLPCSGIIYVEPFRDEKLPSWIAGHVHAFQYLGGVPKTLVPDNLKSGVKR
                     PNFYEPDLNKTYQEMAAYYGTVVLPARVRKPRDKASVENAVLISSRKIIATLRNVQIL
                     SFQDLQQHVRAALEHVNSAPLTGKSESRWTSFLAEEKEFLLPLPESPYELAQWGKAKV
                     QMNCHILCQRKFYSVPFEYLGEDVDVRATQATVEIFYQHQRIASHIRLWGKSDYATVA
                     EHMPPGKLFFADWNRDRFLRWAEKTGVATRRVVEAILDRAVVEQQAYRSCLGVLSLRE
                     KFGPLRLERACGVIASRTSSPTYQQLKNILEKKMDIPRTETPEEGAKKPQGRGFQRGA
                     EYFGGNDHA"
BASE COUNT          498 a          768 c          814 g          614 t
ORIGIN      
        1 gcggcggttc gcaccttttc agcgaaccgc cgcgttgcgt cttcccggac aatgttgcaa
       61 agagcgtttt ttaagagcca cctgctcgtc gatatgagaa ccgcctccgc gctataatag
      121 agccaccggc gcgccggttg agagccaccc ctcagaaacg gaagtggctc tgctccttga
      181 aaacctgaag atcaaatgcg gactgtttcc ctcatggact ttttgccctt gatgacgatc
      241 ttgtacgcct gagaacagag acgatccgtt atcgcctcgg cggcaaccgg gaccggaatc
      301 tggtccagcc attcggctgg ctcgaactgg gacgcgatga tcgtcgcctg gtggttgtgc
      361 cgtcggtcga cgagcgagag cagtatctgg gtgtcctcca tggagatggg aaacagaagc
      421 cattcgtcga tgatcaggag gtcgtacttg ataaactgct ttcgcaagcg atcaaaggct
      481 tccatcccct tggtcctggc cattttcaac tcgtccagca gatcgggcag ctgaacgtag
      541 cgggttttca gcagacgtct gcaggcggca tgtccgagag cctgggacag gaacgacttc
      601 ccgcaacccg tcggtccgac gatcacgatg ttgcgtgcgt gcgggacgta gttgcacgac
      661 gcgagttcca agatgaagtt gcggtcgagc ttccggtcgt cgtcgtatcg gatgtcttcg
      721 atgcaggctg tcggattttc gaatttggcc ccgttgatca ggcgctgcag ccgtccgtgc
      781 tgccgtttgc agaactcgcg gtcgaccagc agcgagaagc ggtcgtggaa gcccatctcc
      841 cggtagcctt cgtcgtcgtt ctgctccttg agcgcctcgg cgatccccgt cagcttcatc
      901 tgcttaagct tgttcacggt ttcatgctca agcatggtcg ttgcctccga agtattccgc
      961 cccgcgctgg aatccgcgcc cttgcggttt cttcgcgcct tcttcgggcg tctcggtccg
     1021 gggtatgtcc atcttcttct caaggatgtt cttgagttgc tggtaggtcg gagatgaagt
     1081 tctcgaagca atgacgccgc aggcccgttc gagccgcagc gggccgaact tctcccgcag
     1141 gctcagcacg ccgagacagg agcggtaggc ctgctgctcg acgaccgccc tgtcgagaat
     1201 ggcttctacc acccgtcgtg tggcgacgcc ggttttctcc gcccagcgca ggaagcggtc
     1261 gcggttccag tcggcgaaga agagcttgcc ggggggcata tgctccgcga cggtcgcata
     1321 gtcgctcttc ccccaaagcc tgatatgcga cgcgatcctc tgatgctggt agaatatttc
     1381 cactgtcgct tgagttgctc gaacgtcgac atcttctccc aggtattcga acggaacgga
     1441 gtagaatttg cgttgacaga gaatgtgaca gttcatctgg accttggctt tcccccactg
     1501 tgccagttcg tagggggatt cgggaagcgg gagcaggaac tccttttctt cggcgagaaa
     1561 ggatgtccag cgactctcgc tcttcccggt caagggggcg gagttgacat gttccagagc
     1621 agcgcgtacg tgctgctgca gatcctggaa ggagagaatc tgaacattgc ggagagtggc
     1681 gataattttt cgggacgaaa tcaggacggc gttctccacg gacgctttat ccctgggttt
     1741 gcggacgcgc gcgggcagta cgaccgttcc gtaataggct gccatctcct gatacgtctt
     1801 gttcaggtcg ggttcgtaga aattgggccg cttgacgccg cttttgagat tgtccggaac
     1861 aagcgtcttg gggacaccgc cgagatattg gaaggcgtgg acatgaccgg cgatccagga
     1921 cggcagcttt tcgtcgcgga acggttcgac atagatgatc ccgctacacg gcagaacggc
     1981 gacgaacaac gacgcctgag acatcttccc gctctcggcg tcgaagaacg cgatccttgt
     2041 gccggcccag tccacctcca gcgagagcgc gggtttgtgc tccagacgga tggttgcctt
     2101 gtgcaccttg gcgtgcaggt gatagtaccg gcggaattgc gtctccatgt aaaagcgttc
     2161 gttgctttga cggcattgtt cgacgtactc ctcccagagc agcttgaggg tgacgtgcgc
     2221 cttcgccagt tcagcatgca catagtcgaa gtcgggcatc ttgaaggtcg cgtcgcggga
     2281 cggcgccgcg atttgctgca agcattgtgc aacggcgtta tcgtccactg cgtccagatc
     2341 cttcagggtg agccagccct gatcccgtgc ctttcgcaga tacagcgaca ccgtgtttct
     2401 ggatactccg tgcgtggcgg cgaccttgcg ctggctcagg ccgctgtaaa cggcccgaac
     2461 tatggtgaga aaatctgaca atgtaatccc tcctgagatg aattccgctt tccggcggta
     2521 cttcgatctt acaggagttc gggagaccgg ctcagtgtcg gcacgtcggg tggctcttca
     2581 ggtccgcgcc aatggctcta acactccgcg ggggcggctc agtttcgccg tgttttccag
     2641 aaggtcagcg catcggcaag cggctgcgta atctgaacgc cggtcagccc gatg
//
LOCUS       DAOSWW010000312         2693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28843_length_2693_cov_3.549280, whole genome shotgun sequence.
ACCESSION   DAOSWW010000312 DAOSWW010000000
VERSION     DAOSWW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28843_length_2693_cov_3.549280"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PK442_06995"
     CDS             complement(<1..711)
                     /locus_tag="PK442_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO87431.1"
                     /translation="MIFLTGDTHGLLEIDRLGTANFPLGERLGRNDLVVILGDFGLLW
                     SDPPRAEERLALRRLEESPWTTLFVDGNHENFSMLDALPEEERFGAPVGVVSPHVLHL
                     RRGYVYTLAGKRCFVFGGARSVDRKGRTRGRSWWPEEIPSAEEYRRGLDSLEASEWSV
                     DWILTHTAPNHILQALDLHKHLHGDPVSAYLDEIFKEVSYQKWFCGHLHRNSRFAKDR
                     IHVLRENIVNGDTEETVSV"
     gene            806..2074
                     /gene="pfp"
                     /locus_tag="PK442_07000"
     CDS             806..2074
                     /gene="pfp"
                     /locus_tag="PK442_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOO87432.1"
                     /translation="MTNPDQKTLAIVCGGGPAPGINSVISSVTIEAKKSGWEVYGIYD
                     GFSNLGKGEKKVTPLTIDAVSRIHSDGGSILRISRFNPTKSDESLRKVVDTLIELGVT
                     HLVTIGGDDTAYAASRVADFAKNNLGLTINFVHVPKTIDNDLPLPEGIPTFGFETARS
                     LGTQIVSNLMEDAKTTGRWYLVVAMGRVAGHLALGIGKSAGATLTLIPEEFPGKEKIP
                     LSLVVDIVTGSIVKRLASGRNYGVAVTAEGLIEKIRLEDLEAADCLEYDDHGHIRYAE
                     INFSDVLKKELLVKLSGMGLKMTVNNKEVGYEVRCAPPNAFDIEYTRNLGYGAFEFLR
                     DGGTNALITIQNNQIVPIPFDQIIDPVTKKTRIRMVNTQSIQYRIARQYMIRLEKKDF
                     NPGIEFERLAVAAKMPPDEFRKQFQYVTDY"
     gene            complement(2078..>2693)
                     /locus_tag="PK442_07005"
     CDS             complement(2078..>2693)
                     /locus_tag="PK442_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOO87433.1"
                     /translation="KGIEVARGRCVRDVLDDPHMPSLLERSGKLVVKPCCSGSTVGVS
                     ILSGTDRLAEALEDAFRYDPRVLVEEYVSGRELTVTVYEKEGVPRCLPVIEIQPVTGF
                     YDYSSKYTPGASEYLVPAPLSEFVLRRVEAASLAAHAALGCAAYSRVDLRLDEEGFPS
                     VLEVNTVPGMTATSLVPKAAAAAGISFGEFLEEVVESSLAARRG"
BASE COUNT          544 a          858 c          828 g          463 t
ORIGIN      
        1 caccgagacg gtctcttccg tatcgccgtt cacgatgttc tcccggagca catggatacg
       61 gtctttcgcg aaacggctgt tgcggtgcag gtggccgcag aaccattttt ggtaggacac
      121 ttctttgaaa atctcgtcca ggtaggccga gacggggtct ccgtgcaggt gcttgtgtag
      181 gtcgagcgcc tgcagaatat ggttgggagc cgtgtgggta agaatccagt cgacgctcca
      241 ctccgaagcc tccagcgagt cgagcccccg gcggtactct tcggccgacg gaatctcctc
      301 gggccaccag gatctccccc tcgtccgccc cttcctgtcc acgctgcgcg cgccgccgaa
      361 gacgaagcag cgcttccccg cgagcgtgta gacgtacccg cggcgcagat ggagaacgtg
      421 cggcgagacg acgccgaccg gcgcgccgaa ccgctcctcc tcggggaggg cgtcgagcat
      481 ggagaagttt tcgtggttac cgtccacgaa aagggtggtc caggggctct cctcaaggcg
      541 gcgcaaggcg agcctctctt ccgcgcgggg agggtcgctc cacagaaggc cgaagtcgcc
      601 caggatgacg accaggtcgt tccgcccaag cctctctccc aaggggaaat tcgccgtccc
      661 gagccgatcg atctcgagaa ggccgtgcgt gtctccggta agaaaaatca tgcgcatccg
      721 ctccttcgcc gtttattata ccgtgcgatg tgctaaaatt ttacgaagtc tttcttttta
      781 tctttcatat tgaaggaggc gcctcatgac gaatccggat caaaaaacac tcgccatcgt
      841 ctgcggagga ggtcccgccc cggggatcaa cagcgtgatc agctccgtca ccatagaagc
      901 aaagaagtcc gggtgggaag tgtacggcat ctacgacggt ttctccaacc tggggaaggg
      961 ggagaagaag gtgacccctc tcaccatcga cgccgtcagc cgaatccact ccgacggagg
     1021 gagcatcctg cggatctccc ggttcaaccc cacgaagagc gacgagtcgc tgcggaaggt
     1081 ggtggacacg ctcatcgagc tgggagtcac gcacctcgtg accatcggag gcgacgacac
     1141 cgcatacgcg gcctcgcgcg tcgccgactt cgcgaagaac aacctcggtc taaccatcaa
     1201 cttcgtccac gtccccaaga cgatcgacaa cgaccttccg ctccccgagg gcatccccac
     1261 cttcggcttc gagactgcgc gttcgcttgg gacgcagatc gtctccaacc tgatggagga
     1321 cgccaagacg acggggcgct ggtacctggt ggtcgcgatg gggcgcgtgg cgggccatct
     1381 cgccctcggc atcggcaaga gcgcgggggc gacgttgacg ctcatccccg aggaattccc
     1441 ggggaaggag aagatcccgc tctccctcgt ggtggacatc gtcacgggct cgattgtgaa
     1501 gcggttggcc tcggggcgca actacggcgt cgcggtcacc gccgaggggc tgatcgagaa
     1561 gatccgcctg gaggatcttg aggccgcgga ctgcctggag tacgacgacc acgggcacat
     1621 ccgctacgcc gagatcaact tctcggacgt cctcaagaaa gagctgctcg tcaagctttc
     1681 cgggatgggc ctgaagatga cggtcaacaa caaagaagtc ggatacgagg tccgctgcgc
     1741 gcccccgaac gccttcgaca tcgagtacac gcggaacctt gggtacgggg cgttcgaatt
     1801 cctccgcgac ggagggacga acgcgctcat caccattcag aacaaccaga tcgtgcccat
     1861 cccgttcgac cagatcatcg acccggtcac gaagaagacc cgtatccgca tggtgaacac
     1921 acagtccatc cagtacagga tcgccagaca gtacatgatc cggcttgaga agaaggattt
     1981 caaccccggc atcgagttcg aacgcctcgc ggtggcggcg aagatgccgc cggacgagtt
     2041 ccgcaagcag ttccagtacg tgacggacta ctgagtctca gccgcgccgg gcggcgaggg
     2101 aggattcgac aacttcttcg agaaattcgc cgaaggagat ccccgccgcc gccgcggcct
     2161 tgggaacaag gctcgtcgcg gtcatcccgg ggacggtgtt cacttcgaga acggagggga
     2221 acccctcctc gtcgagcctg agatcgaccc tgctgtacgc cgcgcacccg agcgccgcgt
     2281 gcgcggcgag ggaggccgct tccaccctgc ggaggacgaa ctccgacagc ggcgccggaa
     2341 cgaggtattc ggaggctccc ggcgtgtact tcgagctgta gtcgtagaat ccagttacgg
     2401 gctgaatctc gatgaccggc aggcaacggg gaacgccctc tttctcatac acggtcaccg
     2461 tcagctccct cccggagaca tactcctcca caagcacccg cgggtcgtag cggaaagcgt
     2521 cttcaagcgc ttcggcgagc cggtcggttc ccgagaggat ggagacgccg acggtgctcc
     2581 ccgaacagca gggcttgacc acgagtttcc ccgagcgttc aagaagagag ggcatgtgcg
     2641 gatcgtccag cacgtcccgg acgcagcgtc cccgcgcgac ctcgatcccc ttc
//
LOCUS       DAOSWW010000313         2687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28923_length_2687_cov_8.457827, whole genome shotgun sequence.
ACCESSION   DAOSWW010000313 DAOSWW010000000
VERSION     DAOSWW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28923_length_2687_cov_8.457827"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1852
                     /gene="istA"
                     /locus_tag="PK442_07010"
     CDS             323..1852
                     /gene="istA"
                     /locus_tag="PK442_07010"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOO87434.1"
                     /translation="MLAMTRINDIRKAYFDEGCTISAIAAKFGIDRKTARKYIEQDDF
                     NKVPPVQSKRNRWPKLEPYMKRIDEWLEDDVKDRSKQRHTAKRVHARLEGTYPDFDCS
                     YRTVASYVKEKKREIYSDVRAALPLEHKAGEAQVDFGSADFYENGTLCSGKYLTVSFP
                     YSNAGYLQLFKGENRECLFEGLKAIFEHLGGVPTRLWFDNMSTVVKKVLKGGKRELCD
                     AFLRFKEHHGFDAAFCNANAGHEKGNVENKVGYHRRNLLVPIPEFERLDRYNEELLRL
                     CDEDHRREHYKRNSTIEALHREDKETLRPLPAVEFDCASYETMKTDLWGKFRISGVHT
                     YSTAPKHAGGRVLVRFTANSVVPLDENHRPITTHRRLYGSLKQEEMDWLPYLTQLSRN
                     PGAMKYSGIWEMLPDDLRGYLEGKEKSGQRDVLRTLALLSERDGFERAVKSISEAVRR
                     GIEDLDSLVALHGHLHGEHPADSMRIDETKLGSLPKLPEVRFAAKVYDGFLSAGGRLP
                     C"
     gene            1846..2589
                     /gene="istB"
                     /locus_tag="PK442_07015"
     CDS             1846..2589
                     /gene="istB"
                     /locus_tag="PK442_07015"
                     /inference="COORDINATES: protein motif:HMM:NF038214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOO87435.1"
                     /translation="MLKNEMKDELLACCKRLKLSRNMADNSELVEAEDREEYLLRLLK
                     LEVEHREKSRKDRLLKAAGFYSWKTFDGYRFDDVKLPSGVTPEYLARVGFVEEKKNLI
                     LYGNVGTGKTHLATAIGIEACRRGLSTGFYRTANLVNRLSEAKKTGRLGKLMTSLSKL
                     DLLICDEWGYVPLDREAAQLLFQIISDSYERRSVIITTNLEFSRWVGIFYDEQMTAAI
                     IDRLVHHSYLLLFDGPSNRVRDSLMRREG"
BASE COUNT          677 a          717 c          821 g          472 t
ORIGIN      
        1 tcagtagcac cgtcctgtaa aactgcgccg ttatttttcc cagcatgttt tccggccttg
       61 ttgcgctctg aaaaccattg ccacaacgat gacgagcagg ttgaaaaggc tttcgagccg
      121 aacaggggct tccctttctt ttgtttttcc gctgtatgat accatattta ttgcataata
      181 gaaacggcag aagttgcaaa gtagaattcc ccagttgtgc aagggagaaa tccccggaaa
      241 tgcaaagggg ccattgaaag caggaccgaa ccccgttacc ctttgagtgt tatccagaca
      301 ctttaagggg agggaatgga ggatgctcgc aatgacccgc atcaatgata tcagaaaagc
      361 gtatttcgac gagggatgca ccatcagcgc catcgcggcg aagttcggaa tcgaccggaa
      421 gaccgcgaga aagtacatag aacaggacga cttcaacaaa gttcctcccg ttcagtccaa
      481 aaggaatcgt tggccgaagc tggagcccta catgaagcgg atcgacgagt ggctcgaaga
      541 cgacgtcaag gatcggagca aacagcggca cacggcgaag agggtgcacg cacggctgga
      601 ggggacatat ccggatttcg actgctctta ccggaccgtt gcctcctacg tgaaggagaa
      661 gaagcgggag atctactccg acgtccgcgc cgctctcccg ttggagcaca aagcgggaga
      721 ggcgcaggtg gatttcggga gcgccgactt ctacgaaaac gggacgctct gctcggggaa
      781 gtatctcacc gtttccttcc cctacagcaa cgcaggatat ctccagctct tcaagggtga
      841 gaaccgggaa tgcctcttcg agggtctgaa ggcgatcttc gagcatctgg gaggggtgcc
      901 gacgcggctg tggttcgaca acatgtcgac cgtcgtgaag aaggtgctca agggaggcaa
      961 gcgcgagctg tgcgacgcct tcctccgctt caaggagcac cacggcttcg atgcggcctt
     1021 ctgcaacgcg aacgcggggc atgaaaaagg gaacgttgag aacaaggtcg ggtaccacag
     1081 acgcaacctt ctcgtgccga tccccgagtt cgagagactg gaccggtaca acgaggagct
     1141 tctgcggctc tgcgacgagg accaccgcag ggagcactac aagagaaaca gcaccataga
     1201 ggcgcttcac agggaggaca aggaaacgct gcgcccgctc cccgccgtcg agttcgactg
     1261 cgcgagctac gagacgatga agacagacct gtgggggaag ttccggatat cgggagtcca
     1321 cacctactcc acagctccca agcacgccgg aggaagagtg ctggtaaggt ttaccgcgaa
     1381 cagcgtggta ccgctggacg aaaaccaccg tccgataacg acgcaccgca gactgtacgg
     1441 ctcgctgaag caggaggaga tggattggct gccctacctg acgcagctct cccgcaaccc
     1501 cggagccatg aagtacagcg gtatatggga gatgctgccg gacgatcttc gcggatacct
     1561 cgaaggaaag gagaagagcg gacaaagaga tgtattgcgg acgctcgccc tgttgagcga
     1621 gcgtgacgga ttcgagcggg ccgtgaaaag catatcggag gcggtaaggc ggggaataga
     1681 ggaccttgac agcctggtgg ccctccacgg acacctgcac ggcgaacacc cggccgacag
     1741 catgcggatc gacgagacga agctggggag ccttcccaag cttccggagg tgcgcttcgc
     1801 ggcaaaggtt tacgacggat tcctttccgc cggaggaaga ctgccatgtt gaaaaacgag
     1861 atgaaggacg aactcctcgc atgctgcaaa cggctcaaac tgagccggaa catggcggac
     1921 aacagcgaac tggtggaggc ggaagacagg gaggagtacc tcctgcgcct cctgaagctc
     1981 gaagtggaac acagggagaa gagcagaaag gaccgtcttc tcaaggctgc cggattctac
     2041 agctggaaga ccttcgacgg ctaccgtttc gacgacgtga agcttccgtc gggcgtgacg
     2101 cccgagtacc ttgcgagagt cggcttcgtc gaggagaaga agaacctcat tctttacggc
     2161 aacgtgggaa ccggaaagac ccaccttgcc acggcgatag ggatagaggc gtgcagaagg
     2221 gggttgagca ccggattcta ccgcacggcc aacctggtga accgcctctc cgaggcgaag
     2281 aagactggca ggctgggaaa gctgatgacg tcgctctcaa aactcgacct actgatctgc
     2341 gacgaatggg ggtatgtccc cctggaccgg gaggccgcgc agctgctctt ccagataatc
     2401 tcggacagct acgaacgaag gagcgtgata atcacgacga accttgaatt cagcagatgg
     2461 gttggaatct tctacgacga gcagatgacg gcggcgataa tagaccgcct tgtacaccac
     2521 agttacctgc tgctattcga cggcccgagc aaccgggtga gagactccct gatgcgcaga
     2581 gaggggtgac aaaattcccc acccccctgc tggggaaaaa tctttgcaac ttgggggtgc
     2641 ggacaaaatc ttggactctt tttcagagac gggaggatgt ccatgta
//
LOCUS       DAOSWW010000314         2687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28927_length_2687_cov_3.494681, whole genome shotgun sequence.
ACCESSION   DAOSWW010000314 DAOSWW010000000
VERSION     DAOSWW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28927_length_2687_cov_3.494681"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /gene="eno"
                     /locus_tag="PK442_07020"
     CDS             complement(<1..1278)
                     /gene="eno"
                     /locus_tag="PK442_07020"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOO87436.1"
                     /translation="MSSIIGVHGREILDSRGNPTVEVEVWLDSGASGRAAVPSGASTG
                     TYEAVELRDGGPRYLGKGVLNAVNNVNEKIAPELMGFDADDQAEVDAALIELDGTPNK
                     GDLGANAVLGVSLAVARAAADDHDLPLWSWIGGLGPFSLPTPMMNVVNGGAHADNNVD
                     IQEFMLVPHGAETFPEALRMGVETYHTLKKAIHARGYSTAIGDEGGFAPDLKSNREAI
                     DLILEAVEKAGYAPGKDISIALDPAASEFFKDGKYHFAGEKKSFTPEEMVDYYEQLCK
                     DYPILSIEDGMAEDDWAGWSVMTKRLGSKIQIVGDDLFVTNPEKLSRGIEEGVANAVL
                     IKLNQIGSLSETLNVIALAKRYGYATVISHRSGETDDSFISDLAVATGAGQIKTGAPA
                     RIDRVAKYNQLLRIAEELDRAPYARLSQFRGNPN"
     gene            complement(1401..1679)
                     /locus_tag="PK442_07025"
     CDS             complement(1401..1679)
                     /locus_tag="PK442_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOO87437.1"
                     /translation="MRLDKFLKLARLVKRRTVAQEMIEVGAVRINGRQCKPSAEVRVG
                     DSVEIAYPSRFLSLEVLCADEAALKRMPPEEAWSLREERRVDPSERPW"
     gene            complement(1748..>2687)
                     /locus_tag="PK442_07030"
     CDS             complement(1748..>2687)
                     /locus_tag="PK442_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOO87438.1"
                     /translation="TAASLGTSLTEEQALLIRRLTDRCFICYDSDIAGQEASIRGMYV
                     LQKAGLDVRVVEIPEGKDPDDLLSLPDGKALFSKALEDARPLVLHHIHLRREMLRTPE
                     KRRKASEDIISGIAMLSPVDASPYLPAVEAALGVLHHEFIPMLEEQRRKHSSQAREPV
                     RDVPPDEETGEEQQPPDLVEAALCALLWRDCDLRRSADPVEIVSMLSDARLQTLASAL
                     LSASSAEDMEIYWHDIGDTFPMKAIAAGGVYCDELERAHDPYTILVDILSVRKHKREY
                     DLLKTKLSRGTAANDELMRFQKLAMILKSGKGKGTL"
BASE COUNT          491 a          854 c          789 g          553 t
ORIGIN      
        1 gttcggattt cccctgaact gcgagagacg cgcgtacgga gcgcggtcga gctcttcggc
       61 gatgcgaaga agctggttgt acttcgcgac cctgtcgatg cgcgccgggg cgcccgtctt
      121 gatctgtccg gctccggtgg ccacggcgag atcgctgata aaggaatcgt cggtctcgcc
      181 ggaacggtgc gagatgacgg ttgcgtagcc gtagcgcttg gcgagcgcga tgacgttgag
      241 cgtctcggag agcgagccga tctggttgag cttgatgaga acggcgttgg ccactccctc
      301 ctcaatacct ctcgacagct tctccgggtt ggtgacgaag aggtcgtcgc cgacgatctg
      361 gatcttggag ccgaggcgct tcgtcatgac cgaccatccg gcccagtcgt cctccgccat
      421 gccgtcctcg atggagagaa tcgggtagtc cttgcagagc tgctcgtagt agtccaccat
      481 ctcctcggga gtgaaggact ttttctctcc cgcgaaatga tacttcccgt ccttgaagaa
      541 ctccgacgcg gcggggtcga gcgcgatgga gatgtccttg cccggcgcgt atcccgcctt
      601 ctccacggcc tcaagaatca ggtcgatggc ctcgcggttg ctcttcagat cgggggcgaa
      661 gccgccctcg tcgccgatgg ccgtgctgta cccgcgggcg tggatggcct tcttcagggt
      721 atggtacgtc tcgacgccca tacgcagcgc ctcggggaag gtctcagcgc cgtggggaac
      781 cagcatgaac tcctgaatat cgacgttgtt gtccgcgtgc gctccgccgt tgaccacgtt
      841 catcatcggc gtcggcagcg agaagggccc gagacctccg atccacgacc acaggggaag
      901 atcgtggtcg tccgccgccg cgcgggctac cgccagcgac acgccgagaa cggcgttggc
      961 gcccaggtcg cccttgttgg gcgtgccgtc aagttcgatg agcgccgcgt cgacctccgc
     1021 ctggtcgtcg gcgtcgaacc ccatcagttc gggcgctatt ttttcattga cgttgttgac
     1081 cgcgttcagc actcccttgc cgagatagcg gggaccgccg tcgcgaagct ccaccgcctc
     1141 gtacgttcct gtggacgccc ctgacggaac agccgctctt ccggaagcgc cgctgtcaag
     1201 ccacacttca acttcaaccg ttggattgcc gcgagaatcg agtatctccc ggccgtgcac
     1261 tccgataata ctgctcatac acctcaactc cttttcaagt acgctcttcg cccgaagatg
     1321 cttttgacgc accggacgat tccaaaacgc cacacacccg gcccgctggg gaggaactgt
     1381 tcagcttgcc gggcgcagta tcaccacggt ctttccgatg gatccacacg ccgctcctcc
     1441 cgcagagacc atgcctcttc aggcggcatt cgcttcaacg ccgcctcgtc ggcgcacagg
     1501 acctccagcg agagaaaacg cgaggggtac gctatctcaa cggaatcgcc cacacggact
     1561 tccgccgaag gcttgcactg tcttccgttg attcgaaccg ccccgacttc gatcatctcc
     1621 tgagcgactg ttctccgttt gacgagcctt gcaagcttca gaaatttatc caatcgcatc
     1681 gcttcgttcc tccagacaca agaccacagg gaagtatatc aaaaaagccc cctgaatttc
     1741 aattctttca caaagtccct tttcctttcc cgctctttaa aatcatggcc agcttctgga
     1801 agcgcatcaa ttcatcgttt gcggctgttc ccctggagag cttcgtcttg agcagatcgt
     1861 actcccgttt gtgtttccgt acggagagaa tgtccaccag aatcgtgtag ggatcgtgag
     1921 cccgctcaag ttcgtcgcag tagactcctc cggcggcgat cgccttcatc gggaacgtat
     1981 cgccgatgtc gtgccagtag atctccatgt cctcagcgga agacgccgag agcagcgcgg
     2041 acgcaagcgt ctgcagccgc gcgtcggaga gcatcgacac aatttccaca gggtcggccg
     2101 atcggcgaag gtcgcagtcc ctccaaagga gcgcgcacaa cgcagcctcc acaaggtcgg
     2161 gaggctgttg ttcttcgccg gtctcttcgt ccggagggac gtcgcggaca ggctcccgtg
     2221 cctgcgaaga gtgcttcctt cgctgctcct ccagcatcgg gatgaattca tgatgcagca
     2281 cgccgagcgc cgcttcgaca gccggaagat acggcgacgc gtccacaggg gagagcatcg
     2341 cgattccgga gataatgtcc tccgacgctt ttcggcgctt ctcgggagta cggagcatct
     2401 ccctccggag gtggatatgg tggagtacca gcggacgggc gtcttcgaga gctttggaga
     2461 agagcgcctt accgtccgga agcgagagca gatcgtccgg atcctttccc tcgggaattt
     2521 cgacgacgcg gacgtccagt cccgccttct gcagcacgta cattccgcgg atggacgcct
     2581 cctgccccgc gatatccgag tcgtagcaga tgaagcagcg gtcggtgagg cgtcggatca
     2641 gcagcgcctg ttcctccgtg agggaggtgc ccagagaggc cgcagtc
//
LOCUS       DAOSWW010000315         2686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28943_length_2686_cov_3.511973, whole genome shotgun sequence.
ACCESSION   DAOSWW010000315 DAOSWW010000000
VERSION     DAOSWW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28943_length_2686_cov_3.511973"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..492)
                     /gene="fliJ"
                     /locus_tag="PK442_07035"
     CDS             complement(37..492)
                     /gene="fliJ"
                     /locus_tag="PK442_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOO87439.1"
                     /translation="MKEKVDRFERILKTRVKGREEEQLLLAGQKSEEEQIVTRLDTLR
                     SQKERALVSFSMQQGTLISPQDMWFQRKSIDVIEKHICDGDSSLCDVRQEIEATEVRL
                     VEKHREVQIMEKYISSIVEEWRTTLQKNEQTEMDDIAGIRHGSHGRKNQ"
     gene            complement(566..1894)
                     /gene="fliI"
                     /locus_tag="PK442_07040"
     CDS             complement(566..1894)
                     /gene="fliI"
                     /locus_tag="PK442_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOO87440.1"
                     /translation="MTTKTADDELLQILEHRLGSVQLTRINGRIVQVVGLVAESQGPD
                     VRVGDLCSIRYRNSESSLSAEVVGFRGDRVLLMPLGNLKDVGPGCDVLSMERPLGVRV
                     GQALLGRVLDGLGNPLDDKGPIAASDFYPLYAEPPHPLRRKMITSPLPVGVKVIDGML
                     TLGTGQRIGIFAGSGVGKSTLLAMMARYTEADINVIALVGERGREVREFLDRDLGEEG
                     KKRSVLVIATSDQPPLIRLKAALTATAIAEYFRDCGKNVLLMMDSVTRVARAQREVGL
                     AVGEPPTTRGYTPSVFELLPRLLERAGAGEKGSITGVYTVLVEGDDMNEPVADTVRGV
                     LDGHVVLSRKIAARNFYPSVDLLESVSRVMPSIVDEEHLMAAGRVRELLAVYAEAEDL
                     ISIGAYKSGSNPRVDWAVTHLQKVRAFLRQSVGEHVPFDVMAQELKSLVP"
     gene            complement(1875..>2686)
                     /locus_tag="PK442_07045"
     CDS             complement(1875..>2686)
                     /locus_tag="PK442_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOO87441.1"
                     /translation="VEDEQEERVSSPPAAQRKEPDELKELQEEVQRLTSALRDAEREN
                     RDLAARVTLSEKELAKEKERLAVERREMEAAQKSEYESLRAKAAAEGAEEGRKSGREE
                     GIRTAREEIAREYEQKVSSLVALLEKVHSTLAERTEELAALGMPRLIRLWELMLSKML
                     HREAQVSEGAVFPVLRSLLERLSDRDRILVYLNPEDSEQTAERKDVFGDLLRGVKHLE
                     FIPDANVEKGSCIVETNLGIYDARWQTQLEQIHREIEHLFLEGRKNDDENG"
BASE COUNT          472 a          824 c          770 g          620 t
ORIGIN      
        1 ttccctgaac gcgaacaata cccgagagat tcggtctcat tgattcttcc tcccgtgaga
       61 gccgtgacga atgccggcaa tatcatccat ttcggtctgc tcgttcttct gaagcgttgt
      121 ccgccactcc tcaactatcg atgagatata tttttccata atctgcacct cgcgatgttt
      181 ctccaccagg cgcacctccg tcgcttctat ttcctgccgg acatcgcaga gagaggagtc
      241 gccgtcgcag atatgctttt ctatgacatc gatcgacttc cgttggaacc acatatcctg
      301 gggggaaatc aaggttccct gctgcatact gaaggagacg agcgcccgct ccttttggga
      361 gcggagcgta tcgagacgcg ttacaatctg ctcctcttcg ctcttttggc ccgcgagaag
      421 cagctgttcc tcttcccggc cttttactct ggtcttcagt attctttcaa atctatccac
      481 tttttctttc atggcgtcct ccggaataca tcattttcat tccttgtttc ggcactcaac
      541 gactttttct tgattcatcg cggtcttacg gcacgaggct cttcagctcc tgcgccatga
      601 cgtcgaaagg cacatgttcc cctactgatt ggcgaagaaa ggcgcggacc ttctgcagat
      661 gcgttacggc ccagtcgacc ctggggttgc ttccgctctt gtacgcgccg atgctgatca
      721 ggtcttccgc ctcggcgtag actgcgagca gctcgcggac cctcccggcg gccatgaggt
      781 gttcttcgtc gactattgag ggcatgacgc ggctgacgct ctcaagcaga tccaccgacg
      841 gatagaagtt cctcgcggcg attttccgcg agagcacgac gtgtccgtca agaacgccgc
      901 ggactgtgtc ggcgacgggc tcgttcatgt cgtctccctc aacgaggacc gtatagactc
      961 ccgtgatgct ccctttctcg ccggcgcccg ccctttcgag gagacgcggc agaagctcga
     1021 agacggacgg cgtgtatccg cgcgttgtcg ggggctcgcc gacggccagg ccgacctcgc
     1081 gctgcgccct cgccacgcgg gtgacggagt ccatcatcaa gaggacgttt ttgccgcagt
     1141 cgcggaagta ttcggcgatc gccgtggcgg tgagggcggc tttcagacgg atgagcggag
     1201 gctggtcgga cgttgcgatg acaaggacgg agcgtttttt tccttcttct ccgagatcgc
     1261 ggtcgagaaa ctcgcgcacc tccctccccc gttcgccgac gagggcgatg acgttgatat
     1321 cggcctcggt gtagcgcgcc atcatggcga gaagggtgct ctttcccact ccggaaccgg
     1381 cgaagatgcc gattcgctga cctgttccca gtgtgagcat gccgtcgatg accttgacgc
     1441 ctacaggaag cggggacgtt atcatcttgc ggcgcagcgg gtggggaggt tctgcgtaga
     1501 gagggtagaa gtcggaggcg gcgatgggcc ccttgtcgtc aagcgggttt ccgagtccgt
     1561 cgagcacccg cccgagaagg gcctgcccga cgcggactcc gagtggtcgt tccatggaga
     1621 ggacgtcgca ccccgggccg acgtccttga ggttgccgag aggcatcaaa agcaccctgt
     1681 ccccccggaa accgacgact tcggcggaga gcgacgactc gctgttcctg taccgtatgg
     1741 agcagaggtc ccctacgcgg acgtcggggc cttgcgattc ggcgacaagg ccgacaacct
     1801 ggacgatgcg cccgttgatg cgggtgagct gaacgctccc caaccggtgt tccagtattt
     1861 gaagcagttc gtcgtcagcc gttttcgtcg tcatttttcc gtccctcaag gaacagatgc
     1921 tctatctctc tgtgaatctg ctcaagctgc gtctgccacc tggcgtcgta gattcccagg
     1981 ttcgtctcga cgatgcagct ccccttttcc acattggcgt cgggtatgaa ctcaagatgc
     2041 ttgactcccc gaagcaaatc gccgaagacg tcttttcgtt cggcggtctg ctcggagtcc
     2101 tctggattca ggtagacgag aattcggtcg cggtcgctca accgctcaag aagactgcga
     2161 agaaccggga aaacagctcc ttcggagacc tgcgcctcgc gatggagcat tttagagagc
     2221 atgagctccc aaaggcggat aaggcgaggc atgccaagag ctgcaagctc ttccgtccgt
     2281 tcagcaaggg tcgagtgcac cttttcgagg agggcgacga gcgaggaaac tttttgttcg
     2341 tattcacggg caatctcttc ccgcgccgtc cggattcctt cctcgcgacc tgactttcgg
     2401 ccctcctcgg cgccttccgc cgcggccttg gcgcgcagag actcatactc ggacttttgc
     2461 gccgcctcca tctcccgacg ttcgacggcg agacgctcct tctcctttgc aagctccttc
     2521 tcggagaggg tgacgcgagc ggcaaggtcg cggttttccc gttcggcatc ccggagagcg
     2581 gacgtaaggc gttgcacctc ttcctgaagc tccttcagtt cgtccggttc tttcctttga
     2641 gccgcgggcg gcgaggaaac gcgctcctcc tgttcatcct ctacag
//
LOCUS       DAOSWW010000316         2685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_28966_length_2685_cov_3.405703, whole genome shotgun sequence.
ACCESSION   DAOSWW010000316 DAOSWW010000000
VERSION     DAOSWW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28966_length_2685_cov_3.405703"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PK442_07050"
     CDS             complement(<1..570)
                     /locus_tag="PK442_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOO87442.1"
                     /translation="MAKKELTEAQRQTLETVFARARAAEKIIENYDQERVDRMCRCIA
                     WAAANPRTFDRFCKMGVEESGAGDWSGRYGKRHKILGVLRDALRQKSVGIVEVNPEKG
                     LVRYGKPAGLITSLIPMTNPELTPIVTAIYALKARDVVVFSPHPRTRKTTFEVVESMR
                     QGLKSIGEPEDFLQCITEVNMAIVEELMKM"
     gene            complement(649..2448)
                     /locus_tag="PK442_07055"
     CDS             complement(649..2448)
                     /locus_tag="PK442_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOO87443.1"
                     /translation="MRKDLVAFQLVKFLEARGVEKVFGLCGHTVIGFLDALKESKITF
                     ITVRHEQIAAHAADGYSRGKGCRVPGVLLTHLGPGLANATTGVAEAGLNSIPMVVIAG
                     DVPSCYFGRHPHQEVNLHADASQYEIYRPFVKRAWRVDRPELLPEVMDKAFRLAVTGR
                     PGPVLVSVPMDIFSMEIDTRFFEQRMHNMPLLPRPGLDVKTAEDIAALLAEAKSPVLY
                     PGGGVISSGAASALTELATFLEIPVLYTLMGKGSIPDDHPLAVGMTGFWGTEFNNSAA
                     MKADVMMAVGTRLSEADCSSWYFGETFDVPPTKLIHIDINQEEIGRNYTTEIGAVCDA
                     KMALDAIFAAAKRKYPNGVKRPELIGEIAEAKAAYRATIAEAQKSDQLPMRPERILAD
                     LREALPRDGFVVADVGWNKNGVGQQFDIYEPGTFVAPGGLATMGYGPSAALGVKVACP
                     DRKVVALIGDGGMGANVSPFATAAVENIGVVWVVMNNCAFGTIAGLERQHYDHEFGTL
                     FEREGKPYSPDFAAIARAYGIDGYTVERAEDFKPMLEKALASDKPCVLDVRMENAPVV
                     TKGCWNINDIFRKRGEEKPWRVWSWEEIEPWRM"
BASE COUNT          520 a          890 c          692 g          583 t
ORIGIN      
        1 catcttcatg agctcctcca cgatagccat attgacctcg gtgatacact gcagaaaatc
       61 ctcgggttcg ccgatcgact tcagtccttg gcgcatgctc tcgacaacct cgaacgtcgt
      121 ctttctggtt ctggggtgcg gagagaagac gaccacatcc cgagctttca gcgcgtaaat
      181 cgccgtgacg atgggcgtca attcaggatt cgtcatcgga atcagggatg tgatcaggcc
      241 cgcgggcttt ccgtaacgga ccagtccttt ctccgggttg acttcgacga tcccgacgct
      301 cttctggcgc aatgcgtcgc gcagcacccc gaggatcttg tgcctctttc cgtagcggcc
      361 gctccaatct cccgcgccgc tctcttccac gcccatcttg cagaatcggt cgaaggtccg
      421 aggattggcc gccgcccagg caatgcagcg acacatcctg tccacacgct cctggtcgta
      481 gttttctatg atcttctccg ccgcgcgcgc tctcgcgaag accgtctcca gagtctgtcg
      541 ctgcgcttcg gtcagttctt ttttcgccat agcatcgtca tctccttcat ccattcgccg
      601 cgttcagtcc ggcgtacagc tcgaaatttg ccgtggtcgc ttgccggtct acatcctcca
      661 cggctcgatc tcctcccagg accataccct ccacggtttc tcctcgccgc gctttctaaa
      721 aatatcgttg atgttccagc accccttcgt caccacggga gcgttttcca tccggacgtc
      781 gaggacgcag ggtttatcgg acgcgagcgc cttttccagc atcggcttga aatcctccgc
      841 ccgctcgacc gtgtatccgt cgatgccgta cgccctcgca atggcggcga aatcagggct
      901 gtacggcttg ccttcgcgct cgaagagcgt gccgaactca tgatcgtagt gctgtctctc
      961 aaggccggcg atcgtaccga aggcgcagtt gttcatcaca acccagacaa cgccgatatt
     1021 ctccaccgcc gcggttgcaa acggcgagac gttcgctccc atacccccgt cgccgatgag
     1081 cgcgacgacc tttctgtcag ggcaggcgac ctttacgccg agcgccgccg agggaccgta
     1141 ccccatagtc gccaaaccgc cgggtgcgac aaaggttccc ggttcgtaaa tatcgaactg
     1201 ctgcccgacg ccgttcttat tccagccgac gtccgccacc acgaagccgt ctcggggaag
     1261 cgcttcccga agatccgcca ggatgcgctc cggccgcatc ggcaactgat ccgacttctg
     1321 cgcctctgcg atcgtcgcac ggtacgcagc cttggcttcg gcgatttctc cgattaactc
     1381 cgggcgtttc acgccgttcg gatacttccg cttcgcagcg gcaaagatag cgtcgagcgc
     1441 catcttcgcg tcgcacaccg cgccgatctc cgtagtgtag ttacggccga tctcctcctg
     1501 gttgatgtcg atatgaataa gcttcgtcgg gggcacgtcg aaggtctcgc cgaagtacca
     1561 cgagctgcaa tccgcctccg aaagcctcgt accgaccgcc atcatcacgt ccgccttcat
     1621 cgcggcgcta ttgttgaact ccgtccccca gaaaccggtc atgcctacgg ccagcggatg
     1681 atcgtccgga atggagcctt tgcccatgag cgtatagagc accggtatct caagaaatgt
     1741 tgcgagctcc gtcaacgcgg acgccgcacc agaagagatc acgccgcccc ccgggtagag
     1801 caccggactc ttcgcctcgg cgaggagcgc ggcgatatcc tccgctgtct tcacgtcgag
     1861 ccccggcctg gggagaagcg gcatattgtg catccgctgc tcgaagaatc gggtatcgat
     1921 ctccatcgag aagatgtcca tcggcacgga gacgagaacc ggtcccggac gtcctgtgac
     1981 ggcgagacgg aacgccttgt ccatcacctc gggaagcagc tccggtctgt ccaccctcca
     2041 ggcgcgcttc acaaacggcc tgtagatctc gtactggctg gcatccgcat ggaggttgac
     2101 ctcctgatga ggatgacggc cgaaatagca gcttgggacg tcgccggcga tgacgaccat
     2161 agggatagag ttgagccccg cttccgcgac accggtcgtc gcgttggcca gccccggtcc
     2221 caggtgtgtc agcagaacac ccggtacccg gcaccccttt ccgcgggaat agccgtcggc
     2281 cgcatgcgct gcaatctgtt catgccgtac cgtaatgaac gtaatcttgc tctctttcag
     2341 agcatccaga aaaccgatga cagtgtgtcc gcaaagaccg aagaccttct ccacaccccg
     2401 ggcctccaga aacttcacga gctgaaacgc taccaggtct tttcgcatat ccttccgcac
     2461 ctccgttgaa aagttcgcaa gcaaccgtct ttatgatggt tttccccgta cgcgcaagaa
     2521 aggagaattc cgtagaacgg aaacaaaaac catctctaag aaatgagtac acaataatct
     2581 atcgccgtcg cctttgtcaa tacttcctct tctttttttc tgtcacccgg aaaacacctt
     2641 cgttgtatcg gcgcttctcc atcacttcga attcccattg aacgg
//
LOCUS       DAOSWW010000317         2681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29044_length_2681_cov_3.367098, whole genome shotgun sequence.
ACCESSION   DAOSWW010000317 DAOSWW010000000
VERSION     DAOSWW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29044_length_2681_cov_3.367098"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PK442_07060"
     CDS             <1..352
                     /locus_tag="PK442_07060"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOO87444.1"
                     /translation="VKEPRLQELAERAKELLKATKSSLSWVPREENKDADALSNEALD
                     GPAKPAQSCLTGKGADDPAAFPAERLERVTGSIYIAHGSEDYAVDLLHKACTCPSFQH
                     RKRCKHLEAAERLA"
     gene            complement(378..1196)
                     /locus_tag="PK442_07065"
     CDS             complement(378..1196)
                     /locus_tag="PK442_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87445.1"
                     /translation="MNRVLLDIRKLTIDFPPSRKRKAVRALKEFSLRLEAGESVSIVG
                     ESGSGKTTLIRCISGHVTPVSGSVSLFGADLSEASNQELVALRRRCGVVSQDPYGSLP
                     PTLTVLEAVMEPWLLVRGREARQAGEHKARELLDELGIGREEILSSKVRLSLSGGQRQ
                     RVAIARALVLGPELLLCDEPTSMQDASTRGDILRILQRRQETGMSMIFVTHDLFLARK
                     AASRGLVLYRGEAVEEGLCRDFLASPQHPYTKALVAALPKLSSSATYAGESGGR"
     gene            complement(1193..1987)
                     /locus_tag="PK442_07070"
     CDS             complement(1193..1987)
                     /locus_tag="PK442_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87446.1"
                     /translation="MISIRNLSITYGRMTSRGRSGETEAVKNISLSVEKGKLFALAGE
                     SGSGKSSVLMAIPGLLPEGTRVSGEILLDGRNLTALSEEELNALRWRRIALIPQGAMN
                     SFTPVLTVGRHIEEVLDVHLGLSKRAARERTQELFVRAGLDGALASRYPHELSGGQKQ
                     RAAIALALACDPDWLLCDEPTTALDVITQKGIVDTLKDLVETKGVGMLLVSHDLPLAA
                     SVCSRIFIMKGGTIVEEGAPREIVAHPKNPHSAALVNALLELEREE"
     gene            complement(1984..>2681)
                     /locus_tag="PK442_07075"
     CDS             complement(1984..>2681)
                     /locus_tag="PK442_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87447.1"
                     /translation="GRWGLLGTDQRGRDVAALFVAGVRVSLIVGIFATLLATFLGTSF
                     GLAAGYLGGWTDSILMRIVDLLLSIPLLPVLMALAALWGKGLWQLVLILSVFSWMGTA
                     RTVRALVLTLRDAPYIEGLRGLGAPPGYILRKHLLPETLPVLLATMTLGVPGAILSEA
                     GLSFLGLSDPRVISWGRMLHEAHSFGAFTAGAWWMLLPPGLGIALLCLVFLDLGKFLE
                     ENVDPRLRKGVRP"
BASE COUNT          470 a          870 c          893 g          448 t
ORIGIN      
        1 ggtgaaggag cctcgtctgc aggagcttgc tgagcgtgcc aaggagctgc tcaaggctac
       61 gaagagctcg ctctcgtggg ttccgcggga agagaacaaa gatgccgacg cgctctccaa
      121 cgaagcgctc gacggtcctg caaagcctgc tcagagctgt ttgacgggga agggcgcgga
      181 tgatcccgcc gcatttccgg ccgaacgcct ggaacgggtt accggctcca tctatatcgc
      241 ccacggaagc gaagactacg ccgtcgatct gctccacaag gcgtgcacct gtccttcgtt
      301 ccagcacagg aagcggtgca aacacctgga ggctgcggag agattggcgt agcgcgcgcc
      361 gtgaaccggc cgcgccgtca tcttccgccg gactccccgg cgtacgtcgc tgaagaggag
      421 agtttcggaa gcgccgctac cagcgccttt gtgtacgggt gctggggcga agcgaggaag
      481 tcgcggcaga gcccctcttc gaccgcctct ccgcggtaga gtacgagccc tctcgacgcc
      541 gccttccgcg cgaggaagag atcgtgggtg acgaagatca tcgacatccc cgtctcctgc
      601 cgccgctgga gaatccgcag aatatcccct ctcgtcgagg cgtcctgcat gctcgtgggt
      661 tcgtcgcaga gcaggagttc cggtccgagc acgagcgccc tggcgatggc gacccgttgg
      721 cgctggccgc cggagaggga gagcctgact ttggacgata aaatttcctc ccggccgatt
      781 cccagttcgt cgagcagttc ccgcgcctta tgctccccgg cttgccgcgc ttcgcgtcct
      841 ctgacgagaa gccacggctc catcaccgcc tccagcacgg tgagggtagg agggagcgag
      901 ccgtacgggt cctgcgagac gacgccgcat cggcgtctga gcgcgacaag ctcctggttc
      961 gaggcttcgg agaggtcggc gccgaagagc gacacgctcc ccgagaccgg cgtcacgtgt
     1021 cccgagatgc agcggatgag cgtcgtcttc ccgctgccgg actcgccgac gatcgagacg
     1081 ctctccccgg cctcaaggcg aagagagaac tccttgagcg cccgcaccgc ctttcgtttc
     1141 cgcgagggcg gaaagtctat cgtcagcttc cggatgtcga gcagcaccct gttcactcct
     1201 ctctctccag ttcaagcagc gcgttcacca gcgccgccga gtgggggttt ttcggatggg
     1261 cgacgatctc gcggggcgct ccttcctcga cgatcgttcc ccccttcata atgaagatgc
     1321 gcgagcagac cgacgcggca agggggaggt cgtgcgagac gaggagcatt ccgaccccct
     1381 tcgtttcgac gaggtctttc aacgtgtcga cgattccttt ttgtgtgatg acgtcgagcg
     1441 ccgtggtcgg ctcgtcgcag agcagccagt cggggtcgca cgcgagcgcg agcgcgatgg
     1501 cggcccgctg cttctgccct cccgaaagct cgtgggggta tcgggatgcc agggcgccgt
     1561 cgagtcccgc tctgacgaac agctcctgcg tccgttcacg cgccgctctt tttgagagcc
     1621 cgaggtggac gtcgagcacc tcctcgatgt gccgcccgac ggtgagcacc ggcgtgaagg
     1681 agttcatcgc cccctgggga atcagcgcga tgcgcctcca gcggagggcg ttcagctcct
     1741 cctcggagag cgccgtcagg ttcctcccgt ccagcagaat ttcccctgaa acgcgcgttc
     1801 cctccggcag cagccccgga atcgccatga gcacggagct ctttccgctt ccggactctc
     1861 cggccagcgc gaagagcttt cctttttcga cggagagcga gatgttcttc accgcctcgg
     1921 tctcgccgct tcgtccgcgg gacgtcatcc gtccgtacgt gatcgagagg ttgcgtatgg
     1981 agatcatggg cggactcctt tccggagccg ggggtcgacg ttctcttcaa ggaattttcc
     2041 gaggtcgaga aagaccaggc agagcagggc gatgccgagc ccgggaggga gcagcatcca
     2101 ccacgcgccg gcggtgaacg cgccgaagga gtgggcttcg tggagcatcc tgccccagga
     2161 gatgaccctc gggtcggaga gcccgaggaa ggagagcccc gcctcggaga gaatggcgcc
     2221 ggggacgccg agcgtcatcg tcgccagcag caccgggagc gtctccggaa gcaggtgttt
     2281 tcgcaaaatg taccccggag gcgcgcccag gccccgcagc ccctcgatgt acggggcgtc
     2341 gcggagcgtg aggacgaggg cgcggacagt ccgcgcggtc cccatccacg agaagacgga
     2401 gaggatgagg acgagctgcc acagcccctt tccccagagc gcggcgagcg ccatgagcac
     2461 cgggagcagc gggatggaga gcagcaggtc gacgatgcgc atcaggatgc tgtcggtcca
     2521 ccctccgagg tagcctgccg cgagtccgaa ggaggtcccg aggaaggtgg ctaacagggt
     2581 ggcgaagatg ccgacgatga gcgagacccg tacccccgcg acgaagagcg cggcgacgtc
     2641 ccttccgcgc tggtcggttc caaggaggcc ccagcgccct c
//
LOCUS       DAOSWW010000318         2678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29100_length_2678_cov_3.069005, whole genome shotgun sequence.
ACCESSION   DAOSWW010000318 DAOSWW010000000
VERSION     DAOSWW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29100_length_2678_cov_3.069005"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PK442_07080"
     CDS             <1..510
                     /locus_tag="PK442_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87448.1"
                     /translation="DGFMTSVNETAVLARRVNEMLRRYMKVQEDLFKPSLRKILRIPG
                     IYRPIDYAENLRALEELLRELAEVKAAIRREEPDAASPEGKFLGVLRGYVSLMTSAVE
                     KLENICSRLKERSEGGAYGKDEYKSDMAALREIQKKHLESGVALNEMMKTLSRSDPPK
                     ETQDDESKS"
     gene            741..1394
                     /locus_tag="PK442_07085"
     CDS             741..1394
                     /locus_tag="PK442_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO87449.1"
                     /translation="MREKKKDQAYETIRTLVLQRRSSDSFVISENSLAAELNMSRTPI
                     REALQRLQMEGFIEIHPHRGIVIPEVSVIEVNETFALRMAIEEFVIKEIIPFMTEQRT
                     AEMDRNLALQKEAVDKGDVSEYLRHDKEFHDHFLRIYSNALIFKTVQRVRDRFISMGA
                     NVLREPGSVQRSFAEHCAISDAVRRGDRGGAAEAMHRHLITGRNNVLSMPGNLGAGI"
     gene            1557..2561
                     /locus_tag="PK442_07090"
     CDS             1557..2561
                     /locus_tag="PK442_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOO87450.1"
                     /translation="MYAQPVRKEATLSPAKYEKDLLGYFYKTMVTIRSFEQMIEKFFL
                     EGEIPGFVHLYIGEEAIATGVMANLRKTDYIQSTHRGHGHTIAKGADLKRMMAELFGK
                     STGSCKGKGGSMHIADFSVGMLGANGIVGGGFTMATGAALAHKMQKTDGVAVTFFGDG
                     ASNRGTFHEAANMAAAWNLPVIFVCENNQWASTTPYRTTTSVDDIADRAVAYGMPGVI
                     VDGNDVFSVCEAAKELVERARRGEGPSILECKSYRIKGHFVGDPEKYRTKEEVQKVFG
                     ETDPIPRFESKVLKAKAMTKKELETIRNTVEEELKETLEFARTSPVPEESALFEGLYV
                     "
BASE COUNT          639 a          700 c          820 g          519 t
ORIGIN      
        1 gacggattca tgacatccgt aaacgaaaca gccgttcttg cgcggcgcgt taacgagatg
       61 ctccgaaggt acatgaaggt gcaggaggat ctattcaaac cgtctctgcg caagatactg
      121 cgtataccgg ggatctacag gccgatcgac tacgcggaga atcttcgcgc gctcgaagag
      181 ctgttgcggg agctcgccga agtcaaggcc gctatccggc gcgaagaacc cgatgcggcg
      241 tctccggagg ggaagtttct gggggtgcta cgggggtatg tctccttgat gacctcggct
      301 gtcgagaagc tggagaacat ctgctcccgc ctgaaggaac gaagcgaggg gggggcgtac
      361 ggcaaagatg aatacaagag cgacatggcg gcgctgcgcg aaattcagaa aaaacatctt
      421 gagtccggcg tcgcgctgaa cgaaatgatg aagacgctct cccgcagcga cccgcccaaa
      481 gaaacgcagg acgacgaatc gaagagctga aaacaaagcc cccggagaaa cgctccgagg
      541 gttttttcat tgccttcctt ccctctgccc tgaaagcggc gcatctgttt tcccccttcg
      601 tttctgcggg cacttttttt aaactttatt ttatccttga gaccctcttg acatttccgt
      661 tacacaaagg gtatcctatc aaatgtatga agcttgtatt ttgcttgtat acaggaaagc
      721 gtttcgaggg ggagagcgcc gtgcgggaga agaagaaaga ccaagcctac gaaacaattc
      781 ggacgctggt actccagcgc agatcctccg attccttcgt catctccgag aactccctgg
      841 ccgccgagct gaacatgagc agaacgccga tacgggaagc gctgcagcgt ctccagatgg
      901 aggggttcat cgagatccac ccgcaccgcg gcatcgtcat tcccgaggtc tcggtaatcg
      961 aagtgaacga gacgtttgct ctccggatgg cgatcgagga gttcgtgatc aaagagatca
     1021 tcccctttat gacggagcag cggacggcgg agatggaccg caatctcgcg cttcagaaag
     1081 aggccgtcga caagggcgac gtctcggagt atctccgcca cgacaaagag tttcacgacc
     1141 atttcctccg tatctattcc aacgctctta tcttcaagac agtacagcgt gtaagggacc
     1201 gtttcatctc catgggggcc aacgtcctcc gcgagccggg aagcgttcag cggtcgttcg
     1261 cggagcactg cgccatttcc gacgccgtcc gcaggggcga taggggcggc gccgcggagg
     1321 ctatgcaccg tcatctgatt acaggcagaa acaatgttct ctctatgccg ggaaatctcg
     1381 gcgcagggat ctgacagctc tacgcggccg cctcgttctt cgtgaccggg agaggccgcg
     1441 gcggattcga ggaagggggg gcgctttttt cgaagaaccg cgcatgaaca agaagaagag
     1501 ggccgcgtaa agggccgtat tcggaaaaag accagacttt caacgaggtg atttgtatgt
     1561 atgcgcagcc tgtgcggaaa gaggcgactc tttctcccgc aaaatatgaa aaagatctgt
     1621 tgggctattt ctacaagaca atggtgacga tcaggagttt cgagcagatg atcgagaagt
     1681 tctttcttga aggcgagatt ccgggattcg tccacctgta tatcggcgag gaggcaatcg
     1741 cgacaggcgt catggccaac ttgcgaaaga ccgattatat ccagagcacg caccgcgggc
     1801 acggccatac catcgccaag ggagcggatc tcaaacggat gatggcggag ctcttcggca
     1861 agagcaccgg aagctgcaag ggaaagggcg gttcgatgca catcgccgac ttcagcgtgg
     1921 ggatgctcgg cgcgaacggc atcgtcggcg gcggattcac aatggcgaca ggcgccgcgt
     1981 tggcgcacaa aatgcagaag accgacggcg tcgcggtcac tttcttcggc gacggcgcat
     2041 ccaaccgggg aactttccac gaggcggcga atatggccgc ggcgtggaat ttgccggtga
     2101 tcttcgtctg cgagaacaac cagtgggctt caacgactcc gtatcgcacc acgacttctg
     2161 tggacgacat cgccgaccgg gccgtcgctt atggaatgcc gggcgttatt gtggacggga
     2221 acgatgtttt ctctgtctgc gaggcggcga aggagctcgt cgaaagagcg cgccgggggg
     2281 aggggccttc tattctggag tgcaaatcgt accgtatcaa gggacacttc gtcggcgacc
     2341 ccgagaagta ccgtacgaag gaagaggtcc agaaggtctt cggcgagacc gatcccatcc
     2401 cccgcttcga gagcaaagtg ctcaaggcga aggcgatgac gaaaaaagaa cttgaaacca
     2461 tcaggaatac ggttgaagaa gaactgaaag aaaccctgga gtttgcacgc acctctcccg
     2521 taccggagga gtcggcgctc tttgaagggc tctacgtgta aggcggtgga caagatgaga
     2581 acaatcagtt tttcccaggc gacgctggaa gcgatgcagg aggagatggc ccgggacgag
     2641 aaggtcttcg tcatgggtga ggatatcgta cgtcaggg
//
LOCUS       DAOSWW010000319         2674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29155_length_2674_cov_3.714013, whole genome shotgun sequence.
ACCESSION   DAOSWW010000319 DAOSWW010000000
VERSION     DAOSWW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29155_length_2674_cov_3.714013"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PK442_07095"
     CDS             complement(<1..1245)
                     /locus_tag="PK442_07095"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOO87451.1"
                     /translation="MKRVSILNHVIGPVMRGPSSSHTAGPWRIGRMARELLGARPVLA
                     RFYFHPSSSLAVCYHDQGSDRAFIAGLLEIPLTSSDFECMLEHAPLLGLDARFELHPF
                     PEADHPNSSLLILTGENGKEITLHSRSVGGGSFEIASLNGYHVSITGENFETLIETSS
                     GSDSSVLSIFGERASSSRIHHTNKGVLFHLSTSSPVEPVMLDTLAALPGTVRLFSAPP
                     VMHPLGGDMLFSSAEEAIIMAERDGLTLGEAALRYEAALLGRDESSILSEMEERLEIM
                     LNAVHLGLSGDFSPMKLLLPVAGTLAAAERKGALFLGGPHARAAIRSMAALHTNASGG
                     VVCAAPTGGAAGVLPGVLTTLTEDLKISRKAVLMALWAAGGIGLFHDIRSTFAAEVAG
                     CQVEIGAAGAMAAAAVVEAAGGS"
     gene            1417..2565
                     /locus_tag="PK442_07100"
     CDS             1417..2565
                     /locus_tag="PK442_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87452.1"
                     /translation="MKMFKILMIVVVILGCASVSVGAENEDDSIFAKMWSKAMPKLEQ
                     ALELFERGETLPESKIFGADKQSNNAKYEKLLNEVFDILLDSEFHASRGEYRSLEERI
                     EKSEQKIAELRKERIGAPEESWIPFRQTRSKIDEEVDELKEDIARFRKEKEAIRSSMV
                     AALSAMGVSVDGAKLDILLASVSGDDVIALLLAADSIKKINLQIVDLLRKGEGNIHMA
                     RKYTGVHMVLVDIYLHAHHDVLEKIASEYSPRLDSITSSAQLLKKQAEQMLAATPRGG
                     SQREVLETNVKANERTVQTASLYRDYLRTQQNKLRISAEKLTSDLSVARNTYQTVKTG
                     GDLISLINISMRTLESVFGFVLPDLSTVYDQEVLKEFERITGQIRKGS"
BASE COUNT          666 a          673 c          755 g          580 t
ORIGIN      
        1 gcttcctccg gcggcctcaa caactgcggc tgcggccatc gctcccgcgg cgcctatctc
       61 tacctggcat cccgcaactt ctgcggcgaa agtacttctg atatcgtgaa agagtcctat
      121 gcctccggca gcccaaagcg ccatgagtac agcttttctg ctgattttca ggtcctctgt
      181 gagcgttgtc agaacgccgg gaagcacgcc ggccgcgccg cctgtgggag ctgcgcagac
      241 cactcctcca gaagcgtttg tatgcagcgc cgccatagag cggatagcag cccgtgcgtg
      301 aggccctcca aggaagaggg ctcccttgcg ttccgcggct gcaagcgttc ccgcgacagg
      361 caagagaagc ttcataggtg agaagtctcc ggaaagaccg agatgtactg cgttgagcat
      421 gatctccaac cgctcttcca tctccgatag tatcgaggat tcatcgcgcc caagcagggc
      481 cgcctcgtaa cgaagcgccg cttctccgag tgtgagtcca tctcgctcgg ccatgataat
      541 tgcctcttcc gcactggaga aaagcatatc ccctccaagc ggatgcatta ctggaggcgc
      601 agaaaaaaga cgaactgttc ccggcagagc ggcgagtgtg tcgagcatga ccggctcaac
      661 cggcgaagat gtagacaggt gaaagagaac ccctttgttt gtatgatgga tccgactgga
      721 ggaggctcgt tctccgaaga tggaaagcac cgaagaatcg cttccggatg aggtttcaat
      781 gagcgtttca aagttttcgc cggtgatcga gacgtgatag ccgttcagcg aggctatctc
      841 gaaagaaccg ccgccgaccg atctgctgtg aagcgtgatc tctttcccgt tttctccggt
      901 aaggatgagc agcgagctgt tgggatgatc ggcctcggga aaaggatgca gctcaaaacg
      961 cgcgtcgagc ccgagaagag gcgcatgctc cagcatgcat tcaaaatcgg aagaggtaag
     1021 cggtatttca aggagtcctg caatgaatgc gcgatcgctt ccctgatcgt ggtaacagac
     1081 cgccagagag cttgaaggat ggaagtaaaa gcgcgccagc acagggcggg cgccgagcag
     1141 ttctcgcgcc attcttccga ttcgccatgg acctgccgtg tgggaacttg aaggcccgcg
     1201 cataaccggg ccgatgacat gattcagtat gcttacccgt ttcaaaagct tcacctccgg
     1261 aatgacgttt tgcttaaaaa gagtatatca tttgaagcgc gagcttatgg aaaacgcctg
     1321 atcgtcgagg tcgcacgaaa ctttttttgc ggtacgcagg aatgacgaac aaagaaaagc
     1381 ttttttgtga tagattactc tcacgaggtg ataactatga agatgttcaa gatactcatg
     1441 attgtagtag ttatactggg gtgcgcttcg gtaagcgtcg gggcggaaaa cgaagacgat
     1501 tccatctttg ccaaaatgtg gagtaaggct atgccgaaac tcgaacaggc tctcgagctc
     1561 ttcgagcgtg gggaaactct gcctgaatca aagatattcg gcgccgataa acagtccaat
     1621 aacgcaaagt atgagaagct cttgaatgag gtcttcgaca tccttctcga ttcggagttc
     1681 catgcctcca gaggcgagta ccgttctttg gaagagagaa tagagaaatc ggagcagaag
     1741 attgcggagc tgcgaaagga acgcatcggc gccccggagg agagctggat tcccttcagg
     1801 cagacacgtt ccaaaattga cgaggaagtt gatgagctca aggaggatat tgctcgcttc
     1861 aggaaagaaa aagaggcgat tcgttcttca atggtggctg cgttaagcgc catgggagtt
     1921 agcgtcgacg gggccaaatt ggatatcctg cttgcaagcg tgagcggcga cgatgtcatt
     1981 gctcttctgc ttgccgcgga cagcataaag aagattaatc tccagatagt agatctcctg
     2041 cgcaagggag aagggaatat ccacatggcc agaaagtata ccggcgttca catggttctt
     2101 gtggatatct atcttcatgc gcatcacgac gttcttgaga agatcgcctc ggagtactcg
     2161 ccgcggcttg attcgatcac aagttctgcg cagctcttaa aaaagcaggc ggagcaaatg
     2221 ctggcagcaa caccaagggg cggcagtcag cgcgaagtgt tggagacgaa tgtaaaggcg
     2281 aatgaacgaa cggtccagac ggcgtccctg tacagggatt accttcgaac gcagcagaat
     2341 aagcttcgta tctcggcaga gaaactcacg agcgatcttt cggttgcgcg gaatacatac
     2401 cagacagtaa aaaccggcgg cgatctgata tccctgatta acatctccat gcggacgctc
     2461 gaaagcgtct tcggttttgt gcttccggat ctttccacgg tgtacgacca ggaagtgctc
     2521 aaggagttcg aacgtattac agggcagata cgcaaaggga gttgagcctg aagcgccgtt
     2581 tcccggatga aacagcatct gtaagcggcg cttgcttttt acttgtacgc ctctcaagtg
     2641 ttctgcagcc ctcggattct tgacaatatg cagc
//
LOCUS       DAOSWW010000320         2671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29210_length_2671_cov_3.946101, whole genome shotgun sequence.
ACCESSION   DAOSWW010000320 DAOSWW010000000
VERSION     DAOSWW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29210_length_2671_cov_3.946101"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..700)
                     /locus_tag="PK442_07105"
     CDS             complement(62..700)
                     /locus_tag="PK442_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO87453.1"
                     /translation="MNDDRTPYDALGTLFAKILEPVALYRALFDERGAFRDVVYIDVN
                     PAYEQVMGIRKEDILGRPFREIWSEREREWQDIILQVATTGIYARYEGPSYDTGKYLH
                     AIAFSPTRSVVAVIFLDMTDWKRAEEALQEKEVLLTEYRQELRTLAARLSLAEEHTRR
                     EISVKLHDRVGYSLVSLLTRLRTLAEKHDPDDIAPAIEEVEGLLSDTRSLTL"
     gene            1020..2327
                     /locus_tag="PK442_07110"
     CDS             1020..2327
                     /locus_tag="PK442_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOO87454.1"
                     /translation="MKLEIGKFFVKEMVFGEKTGYSKGVLTINKEEALAVVKEDSHIT
                     EAELHIACPGDEVRMVPVKEAIEMRCKVSGGVMFPGVTNPVMPAGQGRTHALKDCSLI
                     VVGKHWGSFQDGLIDMAGPFQRNTIFGSLKNLVLVGDTDEEFERHEQQKKNRALRWAG
                     HRLAEYVGKAVAALEPEEIEIWELEPVNRRDASVQGLPSVVYVLQPQTQMEAMGYNTL
                     VYGWDGNHMLPTFMHPNELLDGALISGSFMPVSSKMSTYEHCNNPVVKRLMKEHGKTL
                     NFLGLVMSNLNVAMDQKQRSAYMVAQMAKNLGADGAIVAEEGYGNPDVDYIQTIVELE
                     KVGIKTVGISNECTGRDGGSQPLVALDPAANALVSTGNVSQYFELPPMKTVLGELESM
                     ARDGLSGGWEGCVHPDGSLVMENNGMFCSDHISGFSVKTCMDY"
     gene            2357..>2671
                     /locus_tag="PK442_07115"
     CDS             2357..>2671
                     /locus_tag="PK442_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HOO87455.1"
                     /translation="MLKAIHYINQFFGQVGGEDMADYEPEIRTGTVGCTQIFNTLAKD
                     VEVTHTIICGDNFMASRTDEALERIMGMLEGKEFDIFIAGPAFNAGRYGAACGQICKA
                     VKE"
BASE COUNT          540 a          823 c          839 g          469 t
ORIGIN      
        1 gttcggcgag cgaggcgagc gccggttcca ggccgacttc atagagcagc ggcgagctga
       61 tctacagggt cagtgagcgc gtgtcggaga gcagcccctc cacctcctcg atggccgggg
      121 cgatgtcgtc ggggtcgtgc ttttcggcga gggtacgcag gcgcgtgagc agcgagacga
      181 gcgagtaccc cacccggtcg tgcagcttga cggagatctc tctgcgggta tgctcctccg
      241 cgagggagag acgcgcggcg agggtgcgca gctcctgccg gtactccgta aggagaacct
      301 ccttctcctg aagcgcctcc tcggcccgct tccagtcggt catgtcgagg aagatcaccg
      361 cgacgacgga gcgagtgggg ctgaaggcga tggcgtggag gtacttcccc gtgtcgtagc
      421 tgggtccttc gtaccgggcg tagatccctg tggtggccac ctggaggatg atgtcctgcc
      481 actcccgctc cctctccgac catatctcgc ggaaggggcg gccgaggatg tcctctttgc
      541 ggatgcccat cacctgctcg tacgcgggat tcacgtcgat gtagaccacg tcgcggaacg
      601 cgcctcgttc gtcgaagagc gcccggtaga gggccacggg ttccagtatt tttgcgaaga
      661 gggttccgag ggcgtcgtat ggggtccggt cgtcgttcat gccgctcact ccttcccggc
      721 ttgttttctc ccctcgattc tacacgctgc ggtgaacatg aggcaactgc taccatgccg
      781 acggaggttc tccgcccgta ctccccgcgc aacgcgcgtc gaacgccgca aagaggcatg
      841 acaagcggga gcagcgcgct ttgattcctc attgattcat aaaatcgtcc aaagttcagc
      901 ctgagaattc ataatttgcg ctacgctaca atcatacttt gcacgatgcg cgcccctccc
      961 cccttcccct agactactca acggaggaat atgctattcc attccgggag ggtgagccta
     1021 tgaaactgga gataggaaag ttttttgtga aggagatggt cttcggcgag aagaccggct
     1081 acagcaaggg cgtactgacc ataaacaagg aagaggctct tgcggtggtg aaggaagaca
     1141 gccacatcac cgaggctgag ctgcacatcg catgccccgg agacgaggtc cgcatggtcc
     1201 ccgtgaagga ggccatcgag atgcgctgca aggtctccgg gggcgtaatg ttcccgggcg
     1261 tgaccaaccc cgtcatgccc gcagggcagg gaagaacgca cgcgctgaag gactgctcgc
     1321 tgatcgtggt cggcaagcac tgggggagct tccaggacgg cctgatcgac atggccggcc
     1381 cctttcagag gaacaccatc ttcggctcgc tgaagaatct cgtcctcgtg ggcgacacgg
     1441 acgaagagtt cgaacggcac gagcagcaga agaagaaccg cgcgctccgc tgggccggac
     1501 accgtctggc tgagtacgtc ggcaaggcag tcgccgccct cgaaccggag gagatcgaga
     1561 tatgggagct tgagccggtg aaccgccgcg acgcctccgt acagggactc ccttccgtgg
     1621 tctacgtact gcagccgcag acgcagatgg aggctatggg gtacaacact ctcgtctacg
     1681 gctgggacgg caaccacatg ctccccacct tcatgcaccc caacgagctg ctcgacggcg
     1741 cgctgatctc aggcagcttc atgcccgtct cgtcgaagat gtccacctac gagcactgca
     1801 acaaccccgt cgtgaagcgc ctgatgaagg agcacggcaa gacgctcaac ttcctcggcc
     1861 tcgtcatgtc gaacctcaac gtcgccatgg accagaagca gcgctccgcc tacatggtgg
     1921 cgcagatggc caaaaacctc ggcgcggacg gcgcgatcgt cgccgaagaa gggtacggca
     1981 accccgacgt ggactacatc cagaccatcg tcgaattgga gaaggtcggc atcaagaccg
     2041 tcggcatctc gaacgagtgc accggccgcg acggcgggtc gcagcccctg gtggccctcg
     2101 atccggcggc gaacgccctc gtctccaccg gcaacgtctc gcagtacttc gagctgcccc
     2161 cgatgaagac cgtcctcggc gagctggagt cgatggcccg cgacggcctc tccggaggct
     2221 gggaggggtg cgtccatcct gacggctcgc tcgtcatgga gaacaacggc atgttctgct
     2281 cggaccacat cagcggcttc tccgtgaaga cctgcatgga ctattagcga ccagaagagg
     2341 aaagggagga agcagcatgc tcaaggcgat tcattacatc aaccagttct tcgggcaggt
     2401 gggcggcgag gatatggccg actacgagcc cgaaatccgc accggaaccg tcgggtgcac
     2461 ccagatattc aacaccctcg ccaaggacgt cgaggtgacg cacaccatca tctgcggcga
     2521 caacttcatg gcgtcgcgta ccgacgaggc cttggagcgc atcatgggga tgctcgaagg
     2581 caaagagttc gatatcttca tcgcgggccc ggccttcaac gccggacgct acggcgcggc
     2641 gtgcggacag atctgcaagg ccgtgaagga g
//
LOCUS       DAOSWW010000321         2671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29214_length_2671_cov_3.016820, whole genome shotgun sequence.
ACCESSION   DAOSWW010000321 DAOSWW010000000
VERSION     DAOSWW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29214_length_2671_cov_3.016820"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /gene="pdxA"
                     /locus_tag="PK442_07120"
     CDS             complement(<1..932)
                     /gene="pdxA"
                     /locus_tag="PK442_07120"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOO87456.1"
                     /translation="MKRTVIAVSMGDPAGVGPEIALASMADAEVWERGTPLLVGDLSV
                     LEAVKEALGMKMRLRPVEDPADAAGSPESLPVIDLKMVPDLTALSVGKVSALGGKASV
                     AYIQKCVDLCMTGQAAGIATTPINKESLKAAKVHYIGHTEMLAEMSGAPKSYTMFIVD
                     KLRILFHSRHLSLRRAIETLDTDDAVHSIETAAKCLDSIGLPSGTIALAGLNPHSSDG
                     GLFGDEEARILAPAVEEAKRRGINVVGPVPADSVFYFGLQGKYDVVVSLYHDQGHIAS
                     KTYDFYRTVSVTFGLPFIRTSVDHGTAFDIAWRS"
     gene            complement(968..2188)
                     /locus_tag="PK442_07125"
     CDS             complement(968..2188)
                     /locus_tag="PK442_07125"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOO87457.1"
                     /translation="MNGFAVLCDDLTGSSVQSILLKDRGVPVRQIIRLSGSPPSPPGE
                     GEALVINCDTRRRSREEAEKIFMEILGSFPPDIGIAKRIDTTLRGHLGSETEAILKTR
                     PGAVAMVVPAYPSSGRTTVGGYHLLNGSLLERTEVARDPLWPISTSYVPSYFKGLLIA
                     RMHMECVKRSEEEVIVELRKLLSEGARVVVADAMTDSDIEKLASASFASKEDIIPVDP
                     GPFTAALLSRHLSRRSPGTVLAVIGSTSEKTRKQIAWLDGKIPSEEFVFSPSETTEKA
                     ASRLREFLTSLDRTVSFLLLRPDPRVEKGSEERTARTLAFLGKEALTVLGGHVRGILL
                     SGGDTAAMFFEAAGATALLPETEIQPLMMGGKILDGAFAGLKTVTKGGLVGEEDAIYK
                     AALWLRLAPEATRP"
     gene            complement(2193..>2671)
                     /locus_tag="PK442_07130"
     CDS             complement(2193..>2671)
                     /locus_tag="PK442_07130"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO87458.1"
                     /translation="AGISTSVVFFVIATSQSMSWLITVSRVSAELAAWIVSVSSNPFV
                     LVTMINFILLFLGVFLETQAIILLMAPLLLPITASIGMNPLMLGIIMVVNTSVGMITP
                     PMAVNLFVAVSLVKDHNVRIEQITRKILGFLAAELIAIMLISNFPVLSLGLVQLLK"
BASE COUNT          544 a          809 c          793 g          525 t
ORIGIN      
        1 tccgatctcc atgcgatgtc gaaggcggtt ccatggtcga ccgacgtgcg gatgaacggc
       61 agtccgaagg tgacgctcac cgtccggtag aagtcgtagg tcttcgaggc gatatgcccc
      121 tggtcatgat agagcgagac cacgacgtcg tactttccct gaaggccgaa gtagaagacg
      181 ctgtccgccg gaaccggccc gactacgtta atccctctcc ttttggcctc ctcgactgcc
      241 ggagcaagga tgcgcgcctc ctcgtcgccg aagagccccc catccgacga gtgcgggttg
      301 agcccggcga gcgcgatcgt acccgacgga aggccgatgg agtcgaggca cttcgccgcc
      361 gtttcgatgg aatgaaccgc atcgtccgta tcgagcgtct caatggcgcg ccgcaacgag
      421 aggtgacggc tgtggaagag gatgcggagc ttgtcgacga tgaacatcgt gtagctcttc
      481 ggagcgcccg acatctcggc gagcatctcc gtgtgcccga tgtagtggac cttggcggct
      541 ttcagcgact ccttgttgat cggggtggtc gcgatgcccg cagcctgccc tgtcatgcag
      601 aggtcgacgc acttctgaat gtatgctacg gaggctttgc ctccaagtgc ggaaaccttt
      661 cctacggaga gagccgtaag atctggaacc atcttcaggt cgatcaccgg aaggctctcc
      721 ggcgatcccg ccgcgtccgc agggtcctcc acgggccgga gtcgcatttt catacccagc
      781 gcttccttca cggcttcaag gaccgagagg tcgccgacga gcagcggcgt tccccgctcc
      841 caaacttcag catcagccat gctcgcaagg gcgatctccg ggccgactcc tgcggggtcg
      901 cccatgctga ctgcgatcac tgtacgtttc atgaaatatt actccctttc cttccccctc
      961 cgctctttca gggacgagtt gcttcggggg cgagtctcaa ccagagagcg gccttgtaaa
     1021 ttgcgtcttc ttcgccgacg aggccgccct tggtgactgt tttcaaaccg gcgaacgcac
     1081 cgtcgagaat tttgccgccc atcatcagcg gctgtatctc ggtttcggga aggagcgcgg
     1141 tcgctccggc cgcctcgaag aacattgccg ccgtgtctcc tccggagaga agtatacccc
     1201 gaacatgacc tccgagtaca gtaagggctt ctttcccgag gaaggccagg gtacgggcgg
     1261 tccgctcctc cgagcctttt tcgacgcgcg gatccggtcg aagcaggagg aatgagaccg
     1321 tcctgtccaa actcgtcaga aactcgcgga gacgagatgc agccttctcc gtcgtctccg
     1381 aaggcgagaa gacgaactct tccgacggaa tcttgccgtc gagccaggcg atctgttttc
     1441 tggtcttctc cgacgtactt ccgatgactg cgagcacagt acccggactt ctccgggaga
     1501 gatggcgcga aaggagagcc gccgtgaaag ggcccgggtc cacaggtatg atatcctcct
     1561 tggaggcaaa cgaggctgat gccagctttt caatatcgct gtccgtcatg gcgtccgcca
     1621 cgacgacgcg cgccccttca gagagaagtt tcctcagctc gacgatgact tcttcctcgc
     1681 tcctcttgac gcactccatg tgcatccggg cgatgaggag ccccttgaag taggatggaa
     1741 cgtagctggt agagatcggc cagagggggt cccgcgcgac ctcagtccgc tccagcaggg
     1801 agccgttcaa aagatggtac cctccgaccg tcgtcctccc ggaggagggg taggcgggga
     1861 cgaccatggc gactgctccg gggcgggttt tcaggatcgc ctccgtttcg ctcccgaggt
     1921 gtcctctgag cgtggtgtcg atacgttttg cgattccaat atccggcgga aaagacccca
     1981 gaatctccat gaagatcttc tcggcctctt cccgtgatcg tcgccgcgta tcacagttga
     2041 tcaccagcgc ctccccctcc ccgggaggag aaggcggact gccggagaga cgtattatct
     2101 gacggacggg aactccgcgg tccttcaaca aaatggactg gacggaagaa ccggtgaggt
     2161 cgtcgcagag cacagcaaag ccgttcatag cgctatttca acagctgaac aagccccagg
     2221 gaaagaacgg ggaaattact gatgagcatg atcgctatca gttccgccgc gaggaagccg
     2281 agaattttcc gcgtgatctg ctctatgcgc acgttatgat ccttcaccag cgagaccgcg
     2341 acgaacaggt tcaccgccat tggcggagtg atcattccta ccgatgtgtt caccaccatg
     2401 atgatcccga gcatcagcgg attcatgccg atggacgcgg tgatgggaag gagcagcggc
     2461 gccatcagca ggatgatcgc ctgcgtctcc aggaagacgc cgaggaagag gaggataaag
     2521 ttgatcatcg taaccaggac aaacgggttc gacgacacgg aaacgatcca tgcggccagc
     2581 tccgccgata ccctgctcac cgtgatcagc cacgacatcg actgcgacgt ggcgatgacg
     2641 aagaagacga cggaagtgct gatgcccgcc t
//
LOCUS       DAOSWW010000322         2664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29337_length_2664_cov_3.852051, whole genome shotgun sequence.
ACCESSION   DAOSWW010000322 DAOSWW010000000
VERSION     DAOSWW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29337_length_2664_cov_3.852051"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(445..951)
                     /locus_tag="PK442_07135"
     CDS             complement(445..951)
                     /locus_tag="PK442_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87459.1"
                     /translation="MRLLRNGRRLRESETGKRCERWLGGVWCALGGRDYESGKRWSYR
                     EVVSAIRDIRRRRLIFSLLYASVLAGTLTFLKLQGVQSDESASRILRTLFSIAPVVFA
                     LYIKILIARSYDYLSGADAEIRGALSQPLSASKSIFDATLVFLLWNPIMDIRSLEHLG
                     EILRMLIR"
     gene            complement(1152..1565)
                     /locus_tag="PK442_07140"
     CDS             complement(1152..1565)
                     /locus_tag="PK442_07140"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HOO87460.1"
                     /translation="MTAIDRLLEIEARFAEAVEKIVPLEFDGDALKIVLHLKDGGTLR
                     VVEQWKNARLEKYSYYWLAPDNSLRIGWDNVPHHKHVATFPHHTSRRPTRYESSRSLG
                     FFIKHVGTRDTLRPSEETSLEDVLRVVLDMKSERS"
     gene            complement(1566..1898)
                     /locus_tag="PK442_07145"
     CDS             complement(1566..1898)
                     /locus_tag="PK442_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87461.1"
                     /translation="MRGIAEVDEKIENAFMSLPSEDKSAVISHGAAIRLSELNKRAFL
                     AREKVRSFEEKYAVKLPEIEKTGLPDDAGYEMHEDYIMCSHWSDVIEKTEKQIELLRP
                     LLEYGVLR"
     gene            complement(2003..2482)
                     /locus_tag="PK442_07150"
     CDS             complement(2003..2482)
                     /locus_tag="PK442_07150"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HOO87462.1"
                     /translation="MEQYEQHGSNRAEYGARLLDNISSKLIGSGMEGVAARSLRQYRQ
                     FFATYPEIWQTLSAKSSEALIPSSIRQTLPLESPVSSPAREPISVERWDVFRKLSFSH
                     FAEMIAITDETKRLFYEVECIRGNWSIRELKRQIAFEEGMIARWLERLREIDETPVS"
BASE COUNT          568 a          751 c          729 g          616 t
ORIGIN      
        1 gatgatacag atcatcggcc gaacgaagtg cgtcgagtat cgcttggaaa accgatccag
       61 tcatggcagc tccttatcgt gtgcgaaatc cgcatgaaga gctttcgtgc gaggataaca
      121 tctccataag tatagtacat caagttcggt ttttgccaag agggacattt aaattcaaaa
      181 aaaatcgtgc ataaacagtg cgggctgggg aacgcggaag aagcgccgaa accgccgtca
      241 tctccgcttc gcgtatctga ttttcgatga ggagtggaac cagaggttga gatttggcag
      301 acaccgtctg ccgaatctct gggaatatcg aaaaaaaact gtacgggtgc aataaacggg
      361 cgcttttctg tgacgggcgc ttttctcgca gcaaggggta gagaacgcgc ttgacggtgt
      421 gttgcagaaa gggaaaaaga gcggctatct tatcagcatt cgcaggatct ctcccagatg
      481 ttccagagag cggatgtcca tgatcgggtt ccacagcaga aagaccagcg tcgcgtcgaa
      541 gatgctcttg ctcgccgaga gcggctgaga gagcgctcct cgtatctcgg cgtccgcgcc
      601 cgagagatag tcgtagcttc tcgcgatcag aatcttgatg tagagggcga agacgaccgg
      661 cgctatcgag aagagcgttc tcagaatcct cgaagcgctc tcgtcgctct gcacgccctg
      721 aagcttcagg aaggtgagcg ttccggcgag aaccgaggcg tagagcagcg agaagatcag
      781 tcttcttctc cggatgtccc gtattgcgga cacgacctcg cggtagctcc agcgcttgcc
      841 gctctcgtag tcgcgtcccc cgagggcgca ccagacacca ccgagccatc gttcgcaacg
      901 ttttccggtt tcgctttcgc gaaggcgtct tccgttgcga agaaggcgca tcggccatgt
      961 ctccttccgc tcgtcgtagg tccgccgcca tcgggggagt ttcatcggaa accgggcacg
     1021 gcagaatggg ccgccgtctc ttccattcgg gagcagctcc ttgcctgttc atcgagcaag
     1081 ctttttccaa gccgctgaaa acgtgcaagg ttcaccgctg cggcttcggg cattctcatt
     1141 cggccgggct cctaggaacg ttcacttttc atgtcgagca caacgcgcag cacgtcctcc
     1201 agggatgttt cctcggacgg ccgcagcgtg tcccgggtgc cgacatgctt gatgaagaaa
     1261 ccaagcgatc gactcgactc gtacctcgtc ggtcgcctgc ttgtatggtg cggaaaggtt
     1321 gccacgtgct tatgatgagg gacgttgtcc cagccgatcc gaagcgagtt gtccggtgcg
     1381 agccaatagt agctgtactt ctcgaggcgg gcgttcttcc actgctcgac cactcgcagc
     1441 gtgccgccgt cctttaggtg gagaacgatc ttgagcgcat cgccgtcgaa ctccaacgga
     1501 acgatcttct ctacggcttc ggcgaatcga gcctcgatct ccagcagccg gtcgatggcc
     1561 gtcatttatc gtagaactcc gtactcaagc agaggacgca gcagctctat ctgcttctcc
     1621 gtcttctcga tcacatcgct ccagtggctg cacatgatat agtcctcgtg catctcgtat
     1681 ccggcatcgt cgggaaggcc cgtcttttca atctccggca gcttcacggc gtatttttcc
     1741 tcgaaggagc ggaccttctc tcttgccaga aaggcgcgct tgttcagctc ggagaggcga
     1801 atcgccgcgc cgtggctgat caccgcactt ttatcctcgg acggcagcga cataaaagcg
     1861 ttctctatct tctcatcgac ctcggcgata ccgcgcattg ttcatcctcc tcctcgttgt
     1921 cgcggatacc cacagcatcc gattatttca cagtatatca cgatgacttt atcggaggac
     1981 cgtctcccgg atggaaagag cgtcacgaaa cgggcgtctc gtcgatctcc cgcaggcgct
     2041 caagccacct tgcgatcatt ccttcctcga aggcgatctg gcgcttcaat tcgcgaatcg
     2101 accagttgcc gcggatgcac tcgacttcgt agaaaagacg cttggtttcg tccgtaatcg
     2161 caatcatttc tgcaaagtgg ctgaacgaga gtttccgaaa aacatcccag cgctcgaccg
     2221 agattggttc ccgggcgggc gaagaaacgg gtgattctaa aggtaacgtc tgccgaatgg
     2281 aagatggaat cagggcttcc gatgatttgg cagacagtgt ctgccaaatc tcggggtaag
     2341 tcgcaaaaaa ctgcctgtat tgacgcaatg atctggcggc gactccctcc atgccgcttc
     2401 cgattaactt tgacgagatg ttgtccagta gtctcgcgcc gtattcggcc cggttggatc
     2461 catgctgttc gtattgctca atatagcagc ctattctatt tcatgtcgcc agaaaaacat
     2521 accacactga cgtctcatcg ctcttgtagc gacctccccc ttcatgctat tgttctggcc
     2581 gggaagagta tgatcatcgg tacgccggcc gctcgaggag cagggggatt ctctggaaga
     2641 aagatacggt cctgcttcca tgat
//
LOCUS       DAOSWW010000323         2658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29430_length_2658_cov_3.396081, whole genome shotgun sequence.
ACCESSION   DAOSWW010000323 DAOSWW010000000
VERSION     DAOSWW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29430_length_2658_cov_3.396081"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK442_07155"
     CDS             complement(<1..264)
                     /locus_tag="PK442_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87463.1"
                     /translation="MRETENRLPRSETTFVFPQRLSARLDGISAFPVTIVEAPSGFGK
                     TTAVREFLKSLQHNSRQSWYTCFGEHPSKVWEGVCRLFGNVDID"
     gene            complement(445..1638)
                     /locus_tag="PK442_07160"
     CDS             complement(445..1638)
                     /locus_tag="PK442_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87464.1"
                     /translation="MKKTPLLCASALLFVLTCTLPAFATEEQWTTYVNERFGYSVEYA
                     DIYTERDEPENGDGVWLSASEGKYALTLSGGYNVLEEDGERRLRSRLENVAHIVPGSE
                     ESGPDWYRVIYSDDGGKDGNERLFHEYGSINANNWASFILVYPVEERERFAPIVACLE
                     ETLMIAPEDAGGKELRTDAFALVNGRVQKDGTPLDCEVYDIPSGLDNGIANWAVIGTG
                     TSDAITEKETGVWFFGSEGEFITFIPLDNENEYQDLLWSPAGDRLILVRGSGARPDMF
                     FDVYAEGMEKQAEFSGLREEIAWLDDGMRIVFTRIDGIREGGAFPGLSYGLQLSVILY
                     DSAVEETIPLKEASVTKNFSFVSVSEDGEKITILETSVRSPKDWAGEEKINEREISVP
                     VPAAG"
     gene            complement(1750..2298)
                     /locus_tag="PK442_07165"
     CDS             complement(1750..2298)
                     /locus_tag="PK442_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87465.1"
                     /translation="MREKNRLGLFAGIALLLLLAFSSAALAKPDWVTVKYTGTQLRKQ
                     KNTKVGDGNHLLLVHKFDIINNSKNGDIITAIFDRKIRWNGVFTIVQMTSTTQYFFDG
                     KNSTTWKPAHWNVKFGMTGTNPYKGEWYPGQVYKYEHTIPLNKLIKPYDGSWKTTNEA
                     AFKKFNFKFDKWSLDFQVRSHR"
     gene            complement(2392..>2658)
                     /locus_tag="PK442_07170"
     CDS             complement(2392..>2658)
                     /locus_tag="PK442_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87466.1"
                     /translation="RIDDVRETENGTFSMAALRLSAVMYDTVAEELVVLKEATTMKNY
                     WYGGLSDDGGSFVIQEDSVKSEKDWGDKEKITTREIKVEIPAAG"
BASE COUNT          542 a          757 c          657 g          702 t
ORIGIN      
        1 gtcgatatcc acattcccga acagtctgca cacaccttcc cacaccttcg aggggtgttc
       61 gccgaagcat gtgtaccagc tctgtctgct gttgtgctga agactcttta aaaattctct
      121 gacggctgtc gtcttgccga agccggacgg cgcttccact atggtgaccg ggaacgcgga
      181 tattccgtcc aggcgtgccg acaggcgttg agggaagacg aacgtcgtct cgctacgggg
      241 taatcggttt tctgtctctc gcatggcgtc tctctccgtt catcccgata gtaaaatgca
      301 aaactttttg gagacgctcc ggctataatg tcggagcgtc tccaaaaagt ttacagcaaa
      361 cgccgggaat gtgcacagcc ttctgattgt cgctcgttgt tgcaggtgcg caacggcgtc
      421 aacgcggaga aatcgcggga ctctttatcc cgcggcaggc acgggaacgg atatttcccg
      481 ttcgttaatt ttttcttcgc cggcccagtc tttgggagag cggaccgaag tttccaggat
      541 cgtgatcttt tccccgtctt ccgaaacaga tacaaacgag aagttttttg tgacggaagc
      601 ctctttcaaa ggtatcgttt cctccacggc cgaatcatac aggataacgg acagttgcag
      661 accgtaggaa aggccgggaa acgcaccccc ctcgcgaata ccgtcgatcc tggtaaagac
      721 gatgcgcatt ccgtcgtcca gccacgcgat ttcttcgcgc aggccgctga actcggcttg
      781 tttctccatg ccctccgcgt acacgtcgaa gaacatgtcg ggtcttgctc cgcttccccg
      841 gacgaggatc aggcgatcgc ccgcagggct ccaaagaaga tcctggtatt cgttttcgtt
      901 gtccagaggg atgaaggtta taaattctcc ttcggatccg aaaaaccaga cgcccgtttc
      961 tttttccgta atcgcgtcgg atgttcccgt cccgattacg gcccagttcg cgatcccgtt
     1021 gtccagtccg gatggaatat cgtacacttc gcagtcaaga ggcgttccgt ctttctgcac
     1081 gcggccgttt acgagagcaa aagcgtctgt ccggagttcc ttgccgccgg catcctccgg
     1141 tgcgatcatc aacgtttcct ccaggcacgc gactatggga gcgaaacgtt cccgctcttc
     1201 aacggggtag acgaggatga acgaagccca gttgttcgca ttgatacttc cgtattcgtg
     1261 gaagaggcgt tcgttcccgt ccttcccgcc gtcgtcgctg tagatcactc tgtaccagtc
     1321 gggaccgctt tcttcggagc cgggcacgat atgcgccacg ttttcgagtc tgctccgcag
     1381 cctgcgttct ccgtcttctt caaggacgtt gtagcctccc gagagcgtca gcgcgtactt
     1441 cccttccgaa gcgctcagcc agacgccgtc tccgttttcc ggctcatctc tttccgtgta
     1501 gatatcggcg tattccacag agtagccgaa gcgctcgttg acgtaggttg tccactgttc
     1561 ctcggttgca aaagcgggga gcgtgcacgt caaaacaaag agcagcgccg aagcgcataa
     1621 caagggtgtt tttttcaaga ttcatttctc ctctcgatta ttgagacgcc cgggagcatc
     1681 ctttccagcg aaactggttt tctcggcgaa gagcgcagcg gaagaagctc ttttgataca
     1741 acctcaaaac tacctatggc ttcgaacctg aaaatcgaga ctccatttgt cgaacttgaa
     1801 attgaatttt ttgaacgccg cttcgttggt cgtcttccag ctgccgtcat acggcttgat
     1861 aagtttattg agaggtatcg tatgttcgta tttatacacc tgtccggggt accattcgcc
     1921 tttgtaggga ttcgtcccgg tcatgccgaa ctttacgttc cagtgtgccg gtttccatgt
     1981 tgtagaattc ttgccgtcaa aaaaatactg cgtagtactc gtcatttgca cgatcgtgaa
     2041 aacaccgttc cagcgaattt ttcgatcaaa aatcgccgtg atgatgtcgc cgtttttgct
     2101 gttgttgatg atgtcgaatt tgtgcaccag aagcaggtga ttcccgtcgc cgactttcgt
     2161 gtttttttgc ttccgcagtt gcgttcccgt gtacttgacc gtcacccagt cgggttttgc
     2221 cagagcggcg gaagagaatg ccagcaacag cagcaacgct atgcccgcaa acagtccgag
     2281 cctgttcttc tctctcattt atttcccccc atttttataa aaaatcgtat ttgctgcggg
     2341 aaactcctct cccgcagcaa atacgcccaa aaacaggcgt tccgttacgc tttaccctgc
     2401 cgcgggaatc tctactttta tttcccgcgt tgtgattttt tccttgtcgc cccaatcctt
     2461 ttccgacttc acggagtctt cctgaatgac aaacgagccg ccgtcgtccg ataatccgcc
     2521 gtaccagtag ttcttcatcg tagtcgcttc tttcagaacg accagctcct ccgctacggt
     2581 gtcgtacatc accgccgaga ggcgaagagc ggccattgaa aaagtcccgt tttccgtctc
     2641 gcgaacgtcg tcgatccg
//
LOCUS       DAOSWW010000324         2654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29508_length_2654_cov_2.651789, whole genome shotgun sequence.
ACCESSION   DAOSWW010000324 DAOSWW010000000
VERSION     DAOSWW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29508_length_2654_cov_2.651789"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PK442_07175"
     CDS             complement(<1..710)
                     /locus_tag="PK442_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOO87467.1"
                     /translation="MKRVLFSALAVLLLFASAAAAGPVSVFVSILPQKYFVEQVGGER
                     VDVSAMVEKGRDPHVFEPLPAQMEGLSKAKAYFAVGLPFEESLLPRAASLNPGLKIYR
                     IDEGIDKIAGGREHHDEHEDEPEQNDGKHDHHVHEPHREGADPHVWNSPREAILMAGK
                     ILEGLIEIDPDGREEYARRHADFVERLKTLDAEFAGMFRGRQGATFLVFHPAWAYFAR
                     NYGLVEVAIETEGKEPKAA"
     gene            842..2380
                     /locus_tag="PK442_07180"
     CDS             842..2380
                     /locus_tag="PK442_07180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO87468.1"
                     /translation="MNSLLITGGTLWTGRTFVRGYALHIEGERIAAVGPEEEVRAGAP
                     SECRELRLSGEAVLPGFTDAHLHLTTWAKQKTLLDLGGATSLAEVLELVREEARDTPS
                     DRWIRGWNYNETLWPEGRSVTKDDLDALGIPHPVLLQRVCTHVNVANSRALALVGLES
                     PDGILEERDAIPALRAMERSVFSREGLREALKSACFELASVGVTCVHPCGADDYGMEE
                     DLSLYDELRRRGELPVRVFTYHDALPYPVMPSGFGGEWIRYQGLKIFLDGSLGGRTAA
                     LTAPYADEPAQTGRLNWTDEQVLGMLRAARGKGIQTLLHAIGDRALDQALDCIGKVDE
                     EFGPAALKDRVNHLIVCRPEQRRRLAELGLFCDIQPSFIPSDIAMAPLRLGPERMAWT
                     YRWRSILNECGPLSASSDAPVESVNPMHSIWALVERRGKDGGAPLSPEERLTVEEALP
                     LFTSNPYKAVGRNDAGRLEAGCLADVVVLDRDLRGLSGDDLYSVGVRCTIAGGRATHE
                     EMEA"
     gene            complement(2409..>2654)
                     /locus_tag="PK442_07185"
     CDS             complement(2409..>2654)
                     /locus_tag="PK442_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87469.1"
                     /translation="NMRKIERINVPVLIMASIDDEITPFEHGQRLFEEAAEPKFFLTM
                     NGRHRSHFYRTEQEYVDAIRDFLEFVFGAEQPDLQES"
BASE COUNT          446 a          896 c          838 g          474 t
ORIGIN      
        1 gctgccttgg gctccttgcc ctccgtttcg atggcgacct cgacaaggcc gtagtttctg
       61 gcgaagtaag cccatgcggg gtggaagacg agaaaggtcg ctccctgcct gccgcggaac
      121 atcccggcga attcggcgtc cagcgtttta aggcgctcca cgaagtcggc gtggcgtcgc
      181 gcatactcct ccctgccgtc ggggtcgatc tcgatcagtc cttcgaggat cttccccgcc
      241 atgaggatcg cttcacgggg gctgttccag acgtgcgggt cggcgccctc gcggtggggt
      301 tcatgtacgt gatggtcgtg ctttccgtcg ttctgttccg gctcgtcttc atgttcgtca
      361 tgatgttcgc ggccgcccgc gatcttgtcg atcccctcgt cgatgcggta gatcttcagc
      421 ccggggttca ggctcgccgc cctgggaagc aggctttcct cgaagggaag cccgaccgcg
      481 aagtacgcct tcgccttcga cagcccctcc atctgcgcgg gaagcggctc gaaaacgtgc
      541 ggatcgcgcc ccttctccac cattgccgag acgtcgacgc gctcgccgcc gacctgctcg
      601 acgaagtact tctgcggcag aatggagacg aagacggaaa ccggccccgc cgcagcggcc
      661 gacgcaaaaa gcagcagcac tgccagcgct gaaaaaagaa ctcttttcat aaaaaacacc
      721 tcccgaagat tgcggcatga ttctatcacg attgcccctt tctccacatg ctttcagcgt
      781 gcgccgcgct gttcgtatcc gctataataa caccggaatc ttttgtgtaa ggagggcgcc
      841 catgaacagt ctgctcatca ccggaggaac cctctggacg ggaagaacat tcgtccgggg
      901 atacgcgctg catatagaag gagaacgaat cgccgcggtc ggtccggagg aggaggtccg
      961 cgcgggcgcg ccgtcggagt gccgcgagct tcgtctgtcg ggtgaggcgg tgcttcccgg
     1021 cttcaccgac gcgcacctcc acctgaccac ctgggccaaa cagaagacgc tgctcgatct
     1081 cgggggggcg acgtcgctcg cggaggtcct cgaactggtc cgcgaagagg cgcgggacac
     1141 tccttccgac cgctggatcc gcgggtggaa ctacaacgag acgctctggc cggaggggcg
     1201 ctccgtgacg aaggacgatc tggacgctct cggcatcccg caccccgtgc tgctccagcg
     1261 cgtctgcacg cacgtcaacg tcgcgaacag ccgggctctt gctctcgtcg ggcttgaaag
     1321 tcccgacggc atcctcgaag agcgcgacgc catccccgct ctccgcgcga tggagcggag
     1381 cgtcttctcc cgcgaggggc tccgggaggc gctcaaatcc gcctgcttcg agctggcctc
     1441 cgtgggcgtc acctgcgtcc acccgtgcgg cgcggacgac tacggaatgg aggaggacct
     1501 ctcgctctac gacgagcttc gccgccgggg agagctgccg gtgcgcgtct tcacctacca
     1561 cgacgccctc ccctaccccg ttatgccctc gggcttcggc ggggagtgga tccgctacca
     1621 ggggctcaag atcttcctcg acggcagcct cggcgggcgc accgccgcgc tcaccgcccc
     1681 gtacgcggac gaaccggccc agaccggacg gctgaactgg acggacgagc aggtcctcgg
     1741 gatgctccgc gccgcgcgcg ggaaggggat tcagacgctc ctccacgcca tcggcgaccg
     1801 ggcgctcgac caggcgctcg actgcatcgg aaaggtagac gaagagttcg gccccgccgc
     1861 gctgaaggac agggtcaacc acctcatcgt ctgccgtccg gagcagcgcc gccgtctcgc
     1921 cgaactgggg ctcttctgcg acatccagcc ctcgttcatc cccagcgaca tcgccatggc
     1981 gccgctacgc ctcggcccgg aacggatggc gtggacctac cggtggcgct ccatcctgaa
     2041 cgagtgcggt cctctctccg cctccagcga cgcccccgtc gagtcggtca accccatgca
     2101 ctcgatatgg gcgctcgtcg aacggcgcgg gaaggacggc ggagcgccgc tctcgcccga
     2161 agagcgcctg accgtcgaag aggcgcttcc gctcttcacg tcgaacccgt acaaggccgt
     2221 cggcagaaac gacgccggac gcctcgaagc ggggtgtctg gccgacgtcg ttgtgctcga
     2281 cagggatctg cgcggcctct cgggggacga cctgtactcc gtcggcgtgc gctgcaccat
     2341 cgcgggaggg cgcgcgacgc acgaagaaat ggaggcgtag atacccgcac tcacggtcgg
     2401 gacgccgttc agctttcttg aaggtcgggt tgctccgctc cgaagacgaa ctccagaaag
     2461 tcccggatcg cgtcgacgta ttcctgttcc gtgcggtaga agtgcgaccg gtgccgcccg
     2521 ttcatcgtta aaaagaactt gggctccgcg gcctcttcga agaggcgctg tccgtgttcg
     2581 aacggggtga tctcgtcgtc gatgctcgcc attatcagga cgggcacgtt gatgcgttct
     2641 atcttccgca tgtt
//
LOCUS       DAOSWW010000325         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29571_length_2651_cov_4.174884, whole genome shotgun sequence.
ACCESSION   DAOSWW010000325 DAOSWW010000000
VERSION     DAOSWW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29571_length_2651_cov_4.174884"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..984)
                     /locus_tag="PK442_07190"
     CDS             complement(181..984)
                     /locus_tag="PK442_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO87470.1"
                     /translation="MRRMSLLFLCVAACMLSLGSVARAVEMRIAYEDNEQPPYIYGNS
                     AAIPEKPGLSVEMILMLPEKIPGLEIKLVRMPWLRCIVELENNEVDGIFNASYKKERL
                     RIGRYPTVDGTPDGPVDVDSRIAGMTYSFYVLRKNVGSFQWDGVHFDTIRNAGTIGTP
                     LGYSIAGDLREQGVAVDESGGPETNLAKLAGGRFVAAALLEATVEGLIRSNALYSEIV
                     KVNPPLETKPYYLMLSRAFVEEHPELSRRIWGALREIRDTKTAEILTRY"
     gene            1298..1471
                     /gene="lysW"
                     /locus_tag="PK442_07195"
     CDS             1298..1471
                     /gene="lysW"
                     /locus_tag="PK442_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HOO87471.1"
                     /translation="MSTKVQCVVCEGDVMLPAEVMEGELLTCPDCGTELEVVSLNPLT
                     VAEAPEVQEDWGE"
     gene            1472..2317
                     /gene="lysX"
                     /locus_tag="PK442_07200"
     CDS             1472..2317
                     /gene="lysX"
                     /locus_tag="PK442_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HOO87472.1"
                     /translation="MAHFFIFYTRLRTEEKLLFKAAEERGIPFSAVNVSDGMWPDIPG
                     ASGDVALCRCVSQTQNSALAVLLEARGVLTVNSSEVMALCADKIVTAAKLDAAGIPQP
                     EYAVAFSPEGAIEGAKRLGYPLVIKPATGSWGRLLAKINDRDALEAVAEHKAHMGAAH
                     SVLFMQQYVEKKGFDLRATLLGGEPITVIKRCSEHWITNTARGATPEKYPLSPEIKAL
                     LGRVQDAIGGEVLAVDLFETPEGGWMVNEVNGQPEFRSSDGELTGVDIAGRLVEHVWS
                     LAAQK"
     gene            2337..>2651
                     /locus_tag="PK442_07205"
     CDS             2337..>2651
                     /locus_tag="PK442_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOO87473.1"
                     /translation="MFRAVVWGASGMAGGEVLRILAAHPSIEAVCAVSRGKQGQPVWH
                     THPHLRASFPELAFSAPEETLMHEADVAFLALPHGTSQPLIKEYLDRGVKVADLSGDV
                     RLK"
BASE COUNT          549 a          762 c          801 g          539 t
ORIGIN      
        1 ccgctccacc atggatgccc gcgaagcgtt cgaatccgtt acggaacagg cgaacaccct
       61 tctggaaggt atggaagaga tggagcgtca gctgggccag ttcaaggtgt aagtcaagcc
      121 ggcttttcta ttgaagagaa aggggagcgg ggctgttttt cggccccgct cccctttctt
      181 tcagtacctc gtaagaattt ccgccgtctt tgtatccctg atctcccgga gagcgcccca
      241 aattctccgc gagagttccg ggtgttcttc tacgaaggcc cttgagagca tgaggtaata
      301 cggttttgtc tccagcgggg gattgacttt gacaatttcg ctatagagag cgttggagcg
      361 aatcaaacct tcgacagtcg cttcaaggag cgccgcagcc acaaaccggc ctccggcgag
      421 cttggccagg ttcgtctcgg gccctccgga ctcgtcgacg gcgacgccct gttcacggag
      481 atcaccggca atggagtagc cgagcggggt gccgatagtc cccgcgttcc tgatcgtgtc
      541 gaaatggact ccgtcccatt gaaaggaacc gacgttcttg cgcagtacat agaacgaata
      601 ggtcatgccg gcgatcctgg aatcgacgtc gacaggcccg tcgggcgtgc cgtcgacggt
      661 cgggtatcgt ccgattctca gccgttcttt tttgtagctt gcgttgaaga tgccgtccac
      721 ctcgttgttc tcgagctcca cgatacaacg aagccacggc atgcggacaa gcttgatttc
      781 aagccctgga atcttctccg gaagcatgag gatcatctcc acggaaagcc ccggcttttc
      841 cggtatcgcc gccgagtttc catagatata aggaggctgt tcgttgtctt catacgcgat
      901 cctcatttcg acggcacgag caaccgaccc gagagacagc atgcaggcgg caacgcaaag
      961 aaataacagc gacatccttc gcatacgaaa acacctctct ttttcagggt attgcgctca
     1021 gttttctgcg tcttccgatt gccgagcaca aaggttccat ggttttggac gccctctaaa
     1081 aagaatattt tcattataac accaaaagac gctttcaatt cacaaagtaa agacctcttt
     1141 cagtgcagct cctccaatgc cgcacagagc gtaggattct cgccggaaca cccagtctaa
     1201 aatacagaaa aaaggaacat tatttcccta cttgacgaaa atcaaactca ggtttaaaat
     1261 ccctccattg ttaaattttg ttttggaggg atgttttatg tcaactaaag ttcaatgcgt
     1321 tgtctgcgaa ggagatgtca tgctgcccgc cgaggtcatg gagggcgagc tgcttacctg
     1381 ccccgactgc ggaacggagc ttgaagtggt ctccctgaat ccgctcacgg tcgccgaggc
     1441 ccccgaggtc caggaggact ggggagagta gatggcacac ttcttcatct tctacacccg
     1501 tctccgcacg gaggaaaagc tgctgttcaa agccgccgaa gagcggggaa ttcccttctc
     1561 ggcggtgaac gtgagcgacg gcatgtggcc cgatattccc ggcgcttccg gcgacgtggc
     1621 gctctgccgg tgcgtgagcc agacgcagaa ctccgcgctc gctgtgctgc tggaggcgcg
     1681 cggcgtgctc acggtgaact cctccgaagt aatggccctc tgcgccgaca agatcgtcac
     1741 cgccgcgaag ctcgacgccg ccggaattcc gcagccggag tacgcggtgg ccttctctcc
     1801 ggagggggcg atcgaggggg cgaagcgcct cggctatccg ctggtgatca agcccgcgac
     1861 aggcagctgg gggcgtcttc ttgcgaagat caacgaccgg gacgcgctgg aggccgtcgc
     1921 agagcacaag gcgcacatgg gcgcggcgca ctccgtcctc ttcatgcagc agtacgtcga
     1981 gaagaagggg ttcgacctcc gggcgacgct tcttggcggc gagccgatca cggtgatcaa
     2041 gcggtgcagc gaacactgga tcacgaacac cgcgcgcggc gcgacaccgg agaagtaccc
     2101 gctctcgccc gagatcaagg cgcttctggg cagggtgcag gacgccatcg gcggcgaggt
     2161 gctcgctgtc gaccttttcg agacgcctga aggcggctgg atggtgaatg aagtgaacgg
     2221 acagcccgag ttccgcagct cggacggcga actcacgggc gtcgatatcg cggggcggct
     2281 cgtggagcac gtctggtccc tcgcggcgca gaaatgattc ggaagggatg aagacgatgt
     2341 ttcgagcggt tgtctgggga gcaagcggca tggcgggagg cgaggtcctc cggattttgg
     2401 cggcacatcc gtccatcgag gcggtctgcg cggtgtcgag aggcaaacag gggcagccgg
     2461 tctggcatac gcacccgcat ctgcgggcgt cgttccccga gctggcgttc agcgcgccgg
     2521 aagagactct gatgcacgag gcggacgtcg ccttcctggc gctcccgcac ggaacctctc
     2581 agcctctgat aaaagagtat ctcgaccgtg gggtcaaggt ggccgacctt tccggggacg
     2641 tccgtctgaa g
//
LOCUS       DAOSWW010000326         2644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29700_length_2644_cov_2.577829, whole genome shotgun sequence.
ACCESSION   DAOSWW010000326 DAOSWW010000000
VERSION     DAOSWW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29700_length_2644_cov_2.577829"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..188)
                     /locus_tag="PK442_07210"
     CDS             complement(30..188)
                     /locus_tag="PK442_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87474.1"
                     /translation="MEIKEDVSRILEIPKILNVFASSVRGELGLFALDRLKPQPSLRS
                     LLESQQAG"
     gene            complement(178..762)
                     /locus_tag="PK442_07215"
     CDS             complement(178..762)
                     /locus_tag="PK442_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOO87475.1"
                     /translation="MNVASVFDIVMLLIVITLVLRGLFRGFSGEFFSLLGTVGGIILA
                     WKYSDPLAEKLAEIWPQGGSALLSVSAMALIYIAAVVGAALMCKMVRAFLKFTSLAFI
                     DRILGAGAGLLKGAALILFLYVGITTYSPIIPSEWMGDSVVMRGAHAAWPVVQARMRE
                     WNIFPENFTLPELNLPSLFPQAGEETDGGSSDGN"
     gene            complement(759..1001)
                     /locus_tag="PK442_07220"
     CDS             complement(759..1001)
                     /locus_tag="PK442_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87476.1"
                     /translation="MTSSLRDVTIKVGRSVYHVRTSLDDESLLRVTTLMAEFTGELEH
                     TLDQEKILLLLCLQLGWTLEKIRSKIDGISEELEEQ"
     gene            complement(998..1321)
                     /locus_tag="PK442_07225"
     CDS             complement(998..1321)
                     /locus_tag="PK442_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87477.1"
                     /translation="MTTIGNIEHLVDKLCATVSSLRTERDQLRAQVDELKSKFAEKDL
                     ELIRTGKENQRTLEVLEREKLTLQKEKNQIEGQLKAIYDRISTLMPELSGTLSGAGGE
                     RSARG"
     gene            complement(1334..>2208)
                     /locus_tag="PK442_07230"
     CDS             complement(1334..>2208)
                     /locus_tag="PK442_07230"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOO87478.1"
                     /translation="RGYVELVNYSFLSPSFVELLRLPAADRRARPLLLANPMSVEQSR
                     MRTTLLPGMLRSVEQSVASGWKTAVRVFELGRVFFPLDEGGHEEIERICGVAYGGKDS
                     RLPYGPADADDLFSLKADILVLAESRGVVLRFVQGEEHFGHRGQTVLLYCGEKAVGYL
                     LRLKPSIEKELDCAPLFAFELDLEPFETSALPEFREPSSFPPVYRDISLFVPLAESME
                     RVAEHIREEGGSLLRDVRLFDMYSGKGVPEGFRGLAFSLAYKRDDRTLTDKEVDEVNE
                     RVRSRMSSSGYVLR"
     assembly_gap    2209..2218
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2219..2471)
                     /locus_tag="PK442_07235"
     CDS             complement(<2219..2471)
                     /locus_tag="PK442_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOO87479.1"
                     /translation="MKEASAILSRLGLVEISSTSETATYSVPSWRPDISIEEDLIEEV
                     GRIRGYNETLESRLPQALYGRGDIGRTTRLKGEIRSTLLS"
BASE COUNT          566 a          770 c          732 g          566 t
ORIGIN      
        1 taatatgtcc aaagctttat ccgggcctac tatccagcct gttgtgactc cagcaggctg
       61 cggagcgaag gctgcggctt cagacgatcc agcgcgaaca gtcccagttc gccccggacg
      121 gacgacgcaa agacgttgag tatcttgggt atctcgagaa ttcgtgagac gtcttcttta
      181 atttccatcc gaggagcctc cgtccgtctc ttctcccgcc tgcgggaaga gggatgggag
      241 gttcagctcg ggcagcgtaa agttctccgg gaagatgttc cactccctca tacgcgcctg
      301 gacgaccggc caggcggcat gcgcgccccg catcaccacg ctgtcgccca tccattccga
      361 agggatgatc ggcgaatagg tcgtgatgcc gacatacagg aagaggatga gcgcggcgcc
      421 cttgagaaga ccggcgccgg cgccgaggat cctgtcgatg aacgcgagcg atgtgaactt
      481 caaaaaagcg cgtaccattt tacacatgag cgccgcgccg acgaccgccg cgatatagat
      541 tagcgccatg gccgagaccg agagcagcgc gctccctccc tggggccaga tctccgccag
      601 cttctctgca agcggatcgg aatatttcca tgcgaggatg atgcctccca cagtccccag
      661 caaagagaaa aactctccgg aaaagcccct gaagagcccc cgaagcacca gggtgattac
      721 tatcaacagc attacgatat caaaaactga agccacattc attgttcttc aagctcctcc
      781 gaaattccgt ctattttgga gcgtatcttc tcaagggtcc agccgagctg gagacacagg
      841 agaagaagaa tcttctcctg atcaagcgtg tgctccagct cgccggtgaa ctcggccatg
      901 agcgttgtta ccctcagcag gctctcgtcg tcgagagatg tcctgacgtg gtagacgctc
      961 ctgccgacct tgattgttac atcgcgaagg gatgacgtca ccctctggcg ctcctttccc
     1021 ctccggcgcc ggatagtgtg cccgacaact cgggcatgag ggttgaaata cgatcataga
     1081 tggccttcaa ctgaccttct atctggttct tctccttctg gagggtaagc ttctcccttt
     1141 cgagcacctc cagcgtccgc tgattttcct tccccgtcct gatcagttcg agatccttct
     1201 cggcaaattt gcttttgagc tcgtcgacct gggcgcgaag ctggtcgcgc tcggtccgca
     1261 gcgacgacac ggtcgcgcag agcttgtcga ccagatgctc gatgttgccg atcgtcgtca
     1321 ttcagccccc tccttatcga aggacgtatc cggacgagct cattctgctg cgaactcgct
     1381 cgttgacttc gtccacttcc ttgtcggtga gcgtcctgtc atcccgtttg tatgcgagag
     1441 agaacgccag cccccggaag ccttcgggaa ctcctttgcc tgagtacatg tcgaagaggc
     1501 gcacgtcgcg gaggagtgag cctccttcct cccggatgtg ctccgccacc cgctccatcg
     1561 actccgccag cgggacgaag agggagatat ccctgtagac cggagggaaa gaagaaggtt
     1621 ctctgaattc agggagcgca gacgtttcaa acggctccag atccagttca aaagcaaaga
     1681 gcggagcgca gtcaagctcc ttttcgatcg acggcttgag gcgaagcagg tatcccacgg
     1741 ccttctctcc gcagtacagg agcacggtct gccccctgtg gccgaagtgc tcctctccct
     1801 ggacgaaacg gagaacaacg ccgcggctct cggcgaggac gaggatatcc gctttgagcg
     1861 aaaagagatc gtccgcgtcg gccggtccgt acggaagccg ggagtccttt ccgccgtagg
     1921 cgacgccgca tatacgttca atctcctcgt gcccgccttc atcaagagga aagaagaccc
     1981 tgccgagctc aaagacgcgc accgcggtct tccaccctga agcaacgctc tgctcgacgc
     2041 ttcgaagcat tccgggaaga agcgtcgtac gcatcctcga ctgctccacg ctcatcgggt
     2101 tggccagcaa cagcgggcgg gccctgcgat ccgcggccgg gagacgaagt aactcgacga
     2161 acgaagggga gagaaagctg tagttcacga gctccacgta tcccctggnn nnnnnnnntg
     2221 gagagcagcg tcgatcgtat ctctcccttg agtctggtcg tgcggccgat gtctcctcgc
     2281 ccgtagagcg cctgcggaag acgggactcc agcgtttcgt tgtacccgcg gatcctgccg
     2341 acctcttcga tgaggtcctc ctcgatggag atgtcgggac gccacgaggg aacggaatat
     2401 gtcgccgttt cggacgtcga ggaaatttcg acgaggccga ggcgtgaaag aatcgcggac
     2461 gcctctttca tgtcttctgt gaaattttct tcgattaatc tatcgttatt ttgaggtagg
     2521 gaagtttccg ataagtctat taattcaaaa ttagactgaa ttaataaata tttaagaact
     2581 tcaggatgga ctggtttgct gtgtgttggg tcttgataga acgaattagc aaaaatattg
     2641 agag
//
LOCUS       DAOSWW010000327         2641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29748_length_2641_cov_4.281516, whole genome shotgun sequence.
ACCESSION   DAOSWW010000327 DAOSWW010000000
VERSION     DAOSWW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29748_length_2641_cov_4.281516"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1347
                     /gene="trmD"
                     /locus_tag="PK442_07240"
     CDS             85..1347
                     /gene="trmD"
                     /locus_tag="PK442_07240"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOO87480.1"
                     /translation="MHVTIVTAFPEFFEKFLSTSMIGRGIKNGLLHVDVVDLRNFGLG
                     NYRQIDDYAFGGEGGMVLLPEVLERALAAIRESSGTKGYVVYPSPQGDLFSQEIVETL
                     AAQEHIILFCGHYEGLDERFVSRCVDREVSMGDFVLTGGEIPAMAIIDAMARLVPGVV
                     GKETAVVEDSFFRGMLDHPHCTRPAEWNGECVPEELLSGDAAAIENWRRNAAVERTLA
                     RRPDLISRANIRPYLPKGIYVELVHFPVLDRRGEKSTAAVTGLDLSDIARSCKTYGID
                     RFFVTTPLASQRDLVKTMTRHWTEGGGANVNPERAEAFRLLKLFPSVAKSVEWILKRE
                     KEIPLIVGTTARKHPGAFHWIEAKRRMLREKRPILLLFGTGSGLHEEVLAECSMVLSP
                     LSGGGGEYNHLSVRTAAGAVLDRFFGWR"
     gene            1503..1850
                     /gene="rplS"
                     /locus_tag="PK442_07245"
     CDS             1503..1850
                     /gene="rplS"
                     /locus_tag="PK442_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOO87481.1"
                     /translation="MNVLNLVEKKFLRSDVPDFRSGDTVRVHVKVKEGSRERIQVFEG
                     VVIARKHGGLSETFTVRKISSGVGVERIFPLHCPSISQIEVKRLGKVRRAKLYYLRDR
                     SGKSARIKERREF"
     gene            1902..1988
                     /locus_tag="PK442_07250"
     tRNA            1902..1988
                     /locus_tag="PK442_07250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1936..1938,aa:Leu,seq:aag)
     gene            2146..>2641
                     /locus_tag="PK442_07255"
     CDS             2146..>2641
                     /locus_tag="PK442_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87482.1"
                     /translation="MNMDWESWLRGEEFPPLKQEDPNKNSGAPLAELLGLPVVLPEET
                     AENRSPAASAGEPLQVSQTGGSVDVEVSDADGTMSSLPQATEIQGESSGQQDVMFPVV
                     SEVVEKTETRRHVPASVSEGEPQETKVSEATEEGSSSDTENTDQTALPETERHDDDFV
                     SSRKA"
BASE COUNT          619 a          601 c          774 g          647 t
ORIGIN      
        1 cccggcggtg aaacaggttg tcggcgaagg gaaccttgaa aaacaagaga tacgtattct
       61 gctcatcgag gggctgtggg agtcgtgcat gtaacgattg ttacagcatt cccggagttc
      121 tttgaaaaat ttctgtccac aagcatgatc ggcagaggca tcaaaaacgg tcttctgcat
      181 gtcgatgtgg tggatctcag gaacttcgga ctcggaaact atcgccagat agacgattac
      241 gctttcggcg gcgaaggcgg aatggtgctt cttccggaag tgcttgagag ggctcttgct
      301 gcaattcggg agtcttcggg tacaaaaggg tatgtggtct atccatcccc tcagggcgac
      361 ctcttttctc aggaaattgt ggagaccctg gcggcacagg agcacatcat tcttttctgc
      421 ggtcattacg aggggctgga cgaacgcttc gtctcacgat gtgtcgacag ggaggtctct
      481 atgggtgatt ttgtcctgac cgggggagag atcccggcga tggcgatcat cgacgcaatg
      541 gcccgccttg ttcccggcgt tgtggggaaa gaaacggcag tagtcgagga ttcatttttc
      601 agaggaatgc tggatcaccc acattgtacg cgccctgcgg aatggaatgg agaatgcgtt
      661 ccggaggaat tgctctccgg cgatgccgca gccattgaga actggcggag aaacgccgcc
      721 gttgaacgta ctctcgcacg ccgtcctgat cttatttccc gtgcgaatat ccgcccctat
      781 ctcccaaagg gaatatatgt ggagcttgtc cactttcccg ttcttgacag aagaggagag
      841 aagagcaccg ctgcggttac cgggcttgat ctttcggata ttgcccgttc gtgcaaaacg
      901 tacggaatcg accgtttctt cgtaaccacg ccgttggcga gccagcgtga tttggtgaag
      961 acgatgactc gtcactggac ggaaggcggc ggggcaaacg tcaatcctga acgagccgaa
     1021 gcgtttcgtt tgctcaaact ctttccctcg gtagcgaaaa gcgtcgagtg gattttgaag
     1081 agagagaaag agattcctct catcgtcggg acaaccgcca gaaagcatcc cggcgcattc
     1141 cactggattg aggcgaaaag acggatgctc cgtgagaaac ggccaattct tttgctcttt
     1201 ggtacaggtt cagggctcca tgaggaagtt ttagcagagt gttcgatggt actgtcgcct
     1261 ctttccggcg gagggggaga gtataatcat ctttccgtga gaaccgccgc aggcgccgtt
     1321 ctggatcggt tttttggatg gcgctagaag gaaatcaaca agttccatgc tcggtgcaag
     1381 aacgatcact tgaggcctgt cggactttat tttgttcagg gagttgtgtt ttcgccgtat
     1441 gtctgcttga gattgtgtaa agataatcga gttttgtgaa agctcaagga gggttcattg
     1501 aaatgaacgt gttaaatctt gtcgagaaga agtttttgag gagcgatgtc cctgattttc
     1561 gttccgggga tactgttcgg gtacatgtga aggttaagga aggttccaga gaacgtattc
     1621 aggtctttga gggagtcgtt attgcgcgta aacacggtgg tcttagcgaa acattcaccg
     1681 tccgcaagat ttcgagcgga gtcggagtgg agcgtatctt cccgctgcat tgcccttcga
     1741 tcagccagat tgaagtgaag cgccttggaa aggtccgccg cgccaagctg tactatctgc
     1801 gtgacagaag cggaaagtcc gcccgaatca aagagagaag agaattttag ttttcctctc
     1861 ttgactttgg gaaaaagccc gcttataata tccgtcgttg cgcgggggtg gcggaacagg
     1921 tagacgcgca aggctaagga ccttgtggct gcaaggctgt gggagttcga gtctccccct
     1981 ccgcaccatg aaatcaaaag ccctcttcct gtatgggaag agggctttct tgtatgccgt
     2041 ggtttttctt cgataaaaac gggagaaaag tttttttccc attgcttgaa gtgcttttct
     2101 tttggtacac ttctttggat ggcagtacgt cgagggtggg tggttttgaa catggattgg
     2161 gaaagctggt tgagggggga agagtttcct cctttgaaac aggaagaccc gaacaaaaat
     2221 agcggtgcgc ctctggcgga gcttcttggg cttcctgttg tcctgccgga agagactgcg
     2281 gaaaaccgta gcccagcagc gagcgccgga gagcctcttc aagtttctca aaccggcggc
     2341 tccgtggatg ttgaagtatc cgacgcagac ggaacaatgt cttctctccc tcaagcgact
     2401 gaaatccagg gtgagtcctc tggtcaacag gacgtgatgt ttcctgttgt ttcagaggtt
     2461 gttgaaaaaa cagaaaccag gcggcatgtt ccagcttccg tatcagaggg agaacctcaa
     2521 gaaacaaaag tctcggaagc gacggaagaa ggaagttcct ctgatacgga gaacacagat
     2581 caaaccgcgc tccccgagac ggagagacac gatgatgact tcgtttcctc gagaaaagcg
     2641 g
//
LOCUS       DAOSWW010000328         2641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29754_length_2641_cov_3.678654, whole genome shotgun sequence.
ACCESSION   DAOSWW010000328 DAOSWW010000000
VERSION     DAOSWW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29754_length_2641_cov_3.678654"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PK442_07260"
     CDS             complement(<1..493)
                     /locus_tag="PK442_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87483.1"
                     /translation="MNAVNEKTRYTKRGLLLRGMLPVFVAAVLLCFAGAARAATFTVT
                     RGDDGGAGTLRNAIGNANPGDTIVFDPSVSVVNVNSYLFVNKPLTITGPVTIKQNVSG
                     QRVLYVTGDCTFNQLTVTGGNTGTGSGSQGAGVFSYANLTMNDCTVSGNAGGGGIHNE
                     KTMT"
     gene            843..1112
                     /locus_tag="PK442_07265"
     CDS             843..1112
                     /locus_tag="PK442_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOO87484.1"
                     /translation="MLAVNYSTYRNKLKEYCDRVTDDNETVIVTRKSEKNVVVMSLDE
                     YNNMMESLRILKNPSYFIELFQSLQQLQQGGIVVKTIEELEAAEQ"
     gene            1109..1372
                     /locus_tag="PK442_07270"
     CDS             1109..1372
                     /locus_tag="PK442_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002452576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOO87485.1"
                     /translation="MNIIFSQKAWEEYVAWQNEDKKIVIKINALIKDIARNGMLSGLG
                     QPELLKHALSGLYSRRISLEHRLIYAQDTHGNLLIVKCSGHYE"
     gene            complement(1406..2167)
                     /locus_tag="PK442_07275"
     CDS             complement(1406..2167)
                     /locus_tag="PK442_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO87486.1"
                     /translation="MELQLIRNATVKMSFAGKMLLLDPYLGEKGSGPSYTGKEHSPVV
                     SLPIALEEVLDGVDAVVVSHVHSDHFDTTAREILPKDLPLLCQPEDEQTMRDLGFTDV
                     HPVAGTLWLDEISVSRTSGRHGSGAVLKDMGVVSGFFFEAPGEPSVYWAGDTVLTDEI
                     RELLRVRMPDVVLTHSCGAVWGEGTLILMDDAQTAEVCRISPESTVVAIHMEAVDHAT
                     VTRKQLRETARKSGLSDERLLIPLDGETVQIWARS"
     gene            complement(2323..2478)
                     /locus_tag="PK442_07280"
     CDS             complement(2323..2478)
                     /locus_tag="PK442_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87487.1"
                     /translation="MTQERKKAEAKLEDTLKPEELKKKKKLGEQDDAELDGISGGEGM
                     DTGKIIP"
BASE COUNT          572 a          758 c          724 g          587 t
ORIGIN      
        1 gcgtcatggt tttttcgttg tgaatgccgc ctccgcccgc gttcccgctc acggtgcagt
       61 cgttcatcgt caggttcgcg tagctgaaca cgcccgcgcc ctgcgacccg cttcccgtcc
      121 ccgtgttgcc gccggtgacc gtcagttggt tgaacgtgca gtcgcccgtc acgtagagaa
      181 ctctctgccc gcttacgttc tgcttgatcg tgacgggacc ggtgatcgtc agaggcttgt
      241 tgacgaagag atagctgttt acgttgacga cggataccga tggatcgaag acgatcgtgt
      301 cgccggggtt cgcgtttccg atggcattcc gaagcgttcc cgcgccgccg tcgtctcccc
      361 tcgttaccgt gaatgtcgcc gccctcgccg ctcccgcgaa gcagagcaga accgccgcaa
      421 cgaataccgg cagcatgccc cgcaacaaaa gccctctctt cgtgtaccgc gttttttcgt
      481 ttactgcgtt catcgttttc cccctctttt catggtcggg ttcatgccgc tccggccaag
      541 cacgataaga agagcgtccg tcctcttttg ggtcgttccg cgagctatca taaaccttca
      601 cacggtgtaa aagtcaagga ggagttttaa agattcgccg tttttaaaat aaacattccc
      661 ggtcaatccg gagtgtaagg ttgcctgctg tcgtcttggg aggtccttca aacgacgtta
      721 ttgaaatgta aaaatttcca catgaccgac tccagttttc agcctgcttg cttttcaact
      781 cgaataaacg tacaataact tgtacaatac ttgtaagaaa acccaaagaa ggaggagagg
      841 acatgctggc agtgaactac tccacgtatc ggaataaatt aaaagagtat tgcgaccggg
      901 tgacggacga caatgaaacc gtgatcgtca ctcgaaagag tgaaaagaat gtcgtcgtca
      961 tgagcctcga cgagtataac aacatgatgg agagcttgag aatcctgaag aatccgtcgt
     1021 acttcatcga actctttcag tcattgcagc agcttcaaca gggtggaatc gtcgtcaaaa
     1081 ccatcgagga gctggaagcg gccgagcaat gaatatcatt ttttcgcaaa aagcatggga
     1141 agagtatgta gcgtggcaga atgaagataa aaagatcgtc attaaaatca acgcgctgat
     1201 caaggatatt gccagaaacg gcatgctgag cgggttagga caacccgagc tcctgaagca
     1261 cgctctttcc ggcctgtata gcagaagaat ttcactggaa caccgtttga tatacgctca
     1321 ggacacgcac ggcaatctgc tgatcgtgaa gtgctcaggt cactacgaat aatccccatt
     1381 cacgaaagaa catgagtttt cctgattagc tgcgggccca gatttggacg gtctctccgt
     1441 cgagcgggat caggaggcgc tcgtcggaga gaccgctttt ccgcgccgtc tcgcggagct
     1501 gcttgcgggt cactgtggcg tggtccaccg cctccatgtg gatggcgacg accgtgcttt
     1561 ccggcgaaat gcggcagacc tcggcagtct gcgcgtcgtc catcaggatg agcgttccct
     1621 cgccccagac cgcgccgcac gagtgcgtca ggacgacgtc gggcatacgg acgcgaagca
     1681 actcgcggat ctcgtcggtg aggacggtgt cccccgccca gtagacgctc ggctctccgg
     1741 gcgcttcgaa gaagaagccc gagacgacgc ccatgtcctt cagcaccgcg cccgaaccgt
     1801 ggcgccccga tgtgcgggag acggaaattt cgtccagcca aagcgttccg gcgacggggt
     1861 gtacatcggt gaagccgagg tcgcgcatgg tctgttcgtc ctcgggctgg cagagcagcg
     1921 gaagatcttt cggaagaatc tcccgggccg ttgtgtcgaa gtggtcggag tgcacatggg
     1981 agacgacgac ggcatccacc ccgtcgagca cctcctcaag agcgatgggc agcgaaacga
     2041 ccgggctgtg ctccttcccg gtgtaggaag gccccgaccc cttctccccc aggtacgggt
     2101 ccagcaacag catttttcct gcgaacgaca tttttacggt ggcattgcgg atgagttgca
     2161 gctccaaaat ataccgcctc cttctgtgcc cgatgatacg ccgcagggag acggacggtc
     2221 aagagaaatt ttcaacgagc aggttctttg gacgactgga gcaaacgcac cctaaaaaag
     2281 ctggaaagag atcgcgaatc tctttccagc tttcgaaaaa cgctacggta tgatcttccc
     2341 cgtatccatc ccctcgccgc cggagattcc gtccagttca gcgtcgtctt gctcgccgag
     2401 cttctttttc ttcttgagtt cctccggctt cagggtatcc tccagtttgg cttccgcctt
     2461 cttccgttcc tgcgtcatcg ctgatcccct ccttgtgatg tgcggtgtgt tttccgctgc
     2521 attttccgca gaagaagcag cggtatcgcc agaagcagcg ctaaaggcga cgcaacgtcc
     2581 gtcgagcagc ctgatcctcc cgaggaggcc gctggcgcct cggccgcgcg gaccgagcac
     2641 a
//
LOCUS       DAOSWW010000329         2638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29803_length_2638_cov_3.980256, whole genome shotgun sequence.
ACCESSION   DAOSWW010000329 DAOSWW010000000
VERSION     DAOSWW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29803_length_2638_cov_3.980256"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK442_07285"
     CDS             <1..358
                     /locus_tag="PK442_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOO87488.1"
                     /translation="CTGLGKILLAWLPEPKILEIVGPSPFPRYTENTVTSTEDLLAQL
                     REIRDREIAWDNEEYVEGLFCIAAPVRGNSGDVVAAMSVALPKFLYAGESRKIEAVGD
                     LLLDVARRFSRSLGYK"
     gene            494..1426
                     /locus_tag="PK442_07290"
     CDS             494..1426
                     /locus_tag="PK442_07290"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOO87489.1"
                     /translation="MKTVILGSGAMGSLYGGMLAEAGFDVTLVDVWKEHIDAVNASGL
                     SIEGIGGDRVIRSIRGTTDPAQAGKADLVIVFVKATATEQAMKGALSLLGPETVVLTL
                     QNGLGNVEKLNETAGAERVMAGITGHGCTLLGPGKIRHAGQGDTILGEPRGQISDRLK
                     RVGGMFEKAGFSVKLSENVIGLIWGKLFVNVGINALTAVTGLKNGRLVELQETEELLE
                     LAVREALEVAQAAGIRIETENPVEHTKKVARLTAANRSSMLQDVTNRRQTEIDVINGA
                     IVNEGKKLGVPTPVNLALTNLVRVRQKTYDEFSV"
     gene            1473..2462
                     /locus_tag="PK442_07295"
     CDS             1473..2462
                     /locus_tag="PK442_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO87490.1"
                     /translation="MKLKGMVVMALACAMVFSLVTFGEAAKYEFKLGHAVSDQHPYHL
                     GAQKFKEIVEKETNGEIEISLFPNNQLGTGERDLLEGLQLGTVDLYVGSTGPMGGFEK
                     RFMMFDFPFLFKDKAQAYAVLDGKIGQYVMGLLDRIGITGLAWYENGFRHFTNSKRPV
                     NSPEDVKGLKLRTMENKIHMALWRAMGADPTPMAWGEVFTALQQGTVDGQENPVPIIF
                     VQKVYEVQKHLALTGHVFSPAMIAIAKAKFDALPEGHKEIIRKAAQESALFQREQITK
                     MESEQVAKLKELGMEVTTPDVTPFREATKAVYDEFKSELGDDAKLLDEILSGK"
BASE COUNT          605 a          664 c          843 g          526 t
ORIGIN      
        1 ctgtacggga cttggaaaga tccttctcgc gtggcttccg gagccgaaga ttcttgagat
       61 tgtaggccca tctcccttcc caaggtatac ggagaacacc gtgacatcga ctgaagatct
      121 gcttgcccag ctccgggaga tcagggatcg cgagatagcc tgggacaacg aggagtatgt
      181 cgagggtctc ttctgtatcg ccgcgccggt acggggtaat tccggggatg tcgtggccgc
      241 gatgagcgtt gcgcttccga aattcctcta cgctggagaa tcccgtaaga ttgaagcggt
      301 aggagatttg ttgctggatg tcgcgagaag attctcccgt tcacttgggt ataaataatc
      361 tacgacgtcg tcctcataag agacggtttt tttatggagc tgtccgcccg attagagagg
      421 caattcggaa ggatgtgcgt atcgcacact ccgtcctcta gggaagaaga acgaattcaa
      481 gtaggaggag aaaatgaaga ccgttatcct gggttcaggc gcaatgggtt ctctttacgg
      541 cggcatgctc gccgaagcgg gattcgacgt aacgcttgtc gacgtctgga aggagcatat
      601 cgacgcggtg aacgcatcgg ggctttcgat agaaggcatc ggcggcgacc gcgttatccg
      661 ctccattcgg ggcacgaccg atcctgcgca ggcggggaaa gccgatctcg tcatcgtctt
      721 cgtaaaagcg accgctacag agcaggcgat gaagggcgct ctgtcgcttc tcgggccgga
      781 gaccgttgtg cttacgctcc agaacggtct cggtaacgtg gagaaactga acgaaacagc
      841 gggagccgag cgggttatgg ccggaatcac ggggcatggc tgtacgcttc tcggtcccgg
      901 aaagatacgc catgcggggc agggggatac gatccttggc gagcctcgcg ggcagataag
      961 cgacaggtta aagagagtcg gcgggatgtt tgaaaaagcg ggtttttccg tgaaactctc
     1021 cgagaacgtc atcggactca tttgggggaa gctgtttgtg aatgtcggaa tcaacgcctt
     1081 gaccgccgtg accggcctca agaacggcag actcgtcgag cttcaggaaa ccgaagagct
     1141 gctggagctt gctgttcggg aggcgttgga agtagcgcaa gcagcgggga tacgcattga
     1201 gacggagaac cccgtcgagc acacaaaaaa ggtggcgcgc ctgacggcgg cgaaccgttc
     1261 ctcgatgctg caggacgtaa caaaccggag gcagacggag atcgacgtga tcaacggcgc
     1321 gatcgtgaac gaagggaaaa agctgggggt gcccactccc gtcaacttgg cgctgacgaa
     1381 cctcgtaagg gtccgtcaga agacctatga cgaattttcc gtgtaactta ccgcggggga
     1441 gacgtccaat tcacgtaagg aggggaagga atatgaagtt gaaaggcatg gttgtgatgg
     1501 cgttggcgtg tgcgatggtt ttctcgctcg tgacgttcgg ggaggcggcg aagtacgagt
     1561 tcaagctcgg acacgccgta agcgatcagc atccgtatca tttgggagcg cagaaattca
     1621 aggagattgt cgagaaggag accaacggcg agatcgagat ctcccttttc ccgaacaacc
     1681 agcttggcac gggggagcgg gatctgctgg aagggcttca gctcggaacg gtcgacctct
     1741 atgtaggatc gacagggccg atgggcggct tcgagaagcg cttcatgatg ttcgacttcc
     1801 cgttcctctt caaggacaag gcgcaggcat acgccgtcct cgacgggaag atcgggcagt
     1861 acgtcatggg gctcctcgac aggatcggca tcaccggtct tgcgtggtac gagaacgggt
     1921 tccgccattt caccaactcc aaacggcctg tgaactcccc cgaggatgtg aaggggctca
     1981 agctgcggac catggagaac aagatccata tggcgctctg gagagccatg ggagccgacc
     2041 cgacgccgat ggcgtggggc gaggttttta ccgcgctgca gcaggggacg gtcgacggac
     2101 aggagaaccc ggtgcccatt attttcgtcc agaaggtgta cgaggtgcaa aaacaccttg
     2161 cgcttacagg tcacgtcttc tctcccgcga tgatcgccat cgcgaaggcg aagttcgacg
     2221 cgcttcccga aggacacaag gagatcatcc gtaaggctgc gcaggaatcg gcgctcttcc
     2281 agcgagagca gattacgaag atggagagcg aacaggttgc caagctgaag gagctcggca
     2341 tggaagtgac gacgcccgac gtgactccct tccgtgaggc gacgaaagcc gtctacgacg
     2401 agttcaagag cgagcttggc gatgacgcga aactcctcga cgaaattctg agcgggaagt
     2461 aatcttctcc cggtattgaa ccggtattcc gccgaaggga gagttgtcct tcagtcttcc
     2521 ttcggcgggc ctttccggag attattgtct gagcgaaagg ggggcattta tgtccttttt
     2581 cagaaaaaca ctcgacaggg tgaacgccct ggcggaatac gcagtctgcg ctctcctc
//
LOCUS       DAOSWW010000330         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29840_length_2636_cov_3.294847, whole genome shotgun sequence.
ACCESSION   DAOSWW010000330 DAOSWW010000000
VERSION     DAOSWW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29840_length_2636_cov_3.294847"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2474
                     /locus_tag="PK442_07300"
     CDS             <1..2474
                     /locus_tag="PK442_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO87491.1"
                     /translation="TIYVEIDRSRMAQLGIGTQEIFNTLQAQNAVAGAGSVEVGPEYI
                     RIAPTGYFSSVDELSNLLLRGGDGRSIRLGDFATVTRGYADPPSSAMRYNGKPALGLG
                     ISNVDGGNVIRIGEAVHKRIMELVPRTPVGMELGLIYYQPDTVADSIRNFVVNLLEAL
                     AIVIGILLVFMGFRSGFLIGFILLLTILATFIVMKIMAIDLHSISLGALIIALGMLVD
                     NAIVVADGILVRMQEGKDGRAAAVEVVKQTQIPLLGATVIAAIAFAPIGLSPDSTGEF
                     CKSLFQVVSISLLLSWVLAVTVTPLAAVSILKGNAAGSEVDPYDTRLYRAYRGFLSFC
                     LARRKFVLLVVAVLMVASFAAFGFVDKSFFPASTSPMFTVDFWTPRGTSVQATLAETR
                     TMERYLLSLPETESVAAYAGEGALRFILTYTPGDPASNYGHLIVTAKDSEAAEALKGK
                     LDAFVRANQPHLDPRIRSFAKGTGGGAKVQVRFLGKDPLALRRMAEQATRVFASDPDA
                     VNIRNNWGERTKVIRPELNESVQLLGLTRRDVANALKMAFTGVSAGLYREGEKLLPIV
                     ARLPQSERGSVGSLEETQIWSGLNRRYIPLSQFVSRVDTVAEDPYVYRINREKALTVE
                     CDSGSERPGALFARLRPKIEALSLPLGTEMEWGGEYENSQKAQGGLMGMIPLGFLGIV
                     VILVMLFNGYRQPLIILLCLPLSVMGLSVGLLVMGKSFDFMALLGFLSLTGMLIKNAI
                     VLIDQIDLEMREGKAPLDAIIDSGVSRTRPVMMAALTTVLGMVPLYFDVLFSAMAVTI
                     MFGLTFATALILVVVPVLYGEALKA"
BASE COUNT          447 a          914 c          797 g          478 t
ORIGIN      
        1 agaccatcta cgtggagatc gaccgctccc gcatggcgca gctgggcatc ggcacgcagg
       61 agatcttcaa cacgctccag gcgcaaaacg ccgtcgcggg cgcgggctcc gtcgaggtag
      121 gaccggagta catccgcatc gccccgacgg gctacttctc ctctgtggac gagctctcga
      181 acctgctgct ccgcggaggc gacgggcgct ccatccgcct cggcgacttc gcgacggtga
      241 ctcgggggta cgccgacccg ccgtcctcgg ccatgcgcta caacggcaag cccgcgctcg
      301 gtctgggcat ctccaacgtc gacgggggca acgtcatccg catcggcgag gccgtccaca
      361 agcgcatcat ggagctcgtc ccccgcacgc ccgtcggcat ggagctcggc ctgatctact
      421 accagccgga caccgtcgcc gactccatcc gtaacttcgt cgtcaacctg ctggaggccc
      481 tcgccatcgt catcggcatt ctgctcgtct tcatgggctt ccgcagcggc ttcctgatcg
      541 gtttcatcct gctgctcacc atcctcgcca ccttcatcgt gatgaagatc atggccatcg
      601 acctccacag catctcgctc ggcgcgctca tcatcgcgct ggggatgctg gtggacaacg
      661 ccatcgtcgt ggcggacggc atcctcgtcc ggatgcagga gggcaaggac ggcagagccg
      721 ccgccgtcga agtggtcaag cagacgcaga tcccgctgct gggcgcgaca gtcatcgccg
      781 ccatcgcctt cgcccccatc ggcctctcgc cggacagtac gggggagttc tgcaagagcc
      841 tcttccaggt tgtcagcatc tcgctgctgt tgagctgggt actcgccgtc accgtcaccc
      901 cgctcgcagc cgtttccatc ctcaagggga acgcagcggg ctcggaagtc gacccgtacg
      961 acactcgcct ctaccgcgcc taccggggct tcctctcctt ctgcctcgcc cggcgcaaat
     1021 tcgtgcttct ggtggtagcg gtgttgatgg tcgcctcctt cgccgccttc ggcttcgtgg
     1081 acaaatcctt cttccccgcc tccacaagcc ccatgttcac tgtggacttc tggaccccgc
     1141 gcggaaccag cgtgcaggcc acgctcgcgg agacgcggac gatggagcgc tacctgctct
     1201 ccctgccgga aaccgagtcc gtcgccgcat acgccgggga gggagcgctc cgcttcatcc
     1261 tcacctatac gcccggcgac ccggcctcca actacggcca cctgatcgtc accgcgaagg
     1321 acagcgaggc ggcggaagcc ctcaagggca agctcgacgc cttcgtccgg gccaaccagc
     1381 cccacctcga cccgcgcatc cgctcgttcg ccaagggaac cgggggcggc gcgaaggtac
     1441 aggtgcgctt cctggggaag gacccgctcg cgctgcggcg aatggccgaa caggcgacgc
     1501 gcgtcttcgc ctccgacccg gacgcggtga acatccgcaa caactggggg gaacgcacga
     1561 aggttatccg ccccgagctg aacgaatccg tccagctcct cggactaacc cgccgcgacg
     1621 tggcgaacgc gctcaagatg gccttcaccg gagtgagcgc ggggctctac cgcgaggggg
     1681 aaaagctatt gcctatcgtg gcgcgccttc cgcagtccga acgggggagc gtggggtcgc
     1741 tcgaagagac gcagatatgg agcggcctca accgacggta catcccgctg tcgcagttcg
     1801 tctcgcgcgt cgacaccgtg gcggaggatc cgtacgtcta ccggatcaac cgggaaaaag
     1861 cgctcaccgt ggagtgcgac agcggctccg agcggccggg agcgctcttt gcacgactgc
     1921 gccccaagat cgaggcgctc tccctgccgc tggggacgga gatggagtgg ggcggcgaat
     1981 acgagaattc gcagaaggcg cagggcggcc tgatggggat gatccctctc ggcttcctcg
     2041 gcatcgtggt cattctcgtc atgctcttca acggctaccg acagccgctt atcatcctgc
     2101 tctgtctgcc gctctcggtc atggggctct cggtggggtt gctcgtgatg ggaaagtcgt
     2161 tcgacttcat ggccctgctg ggcttcctga gcctcaccgg aatgctcatc aaaaacgcca
     2221 tcgtcctgat cgaccagatc gacctggaga tgcgggaagg gaaggccccg ctcgacgcca
     2281 tcatcgactc gggcgtcagc cgcacccgcc ccgtcatgat ggccgcgctc accaccgtgt
     2341 tggggatggt cccgctctac ttcgacgtcc tcttctcggc gatggccgtc accatcatgt
     2401 tcggcctcac cttcgccacg gcgctcatcc tcgtggtcgt cccggtgctc tacggagaag
     2461 cgctgaaggc ataacagcgg aaatactttc gggaacacaa acgaggcggt tcgcaatttt
     2521 cctgcgaacc gcctcgttgc gccttttcgg agaatttcgt tccgatatac cggttttcag
     2581 ttcgtcttcg acgcggcgtc gagttccctg aagaaaaaga tcgcgagcgg caggca
//
LOCUS       DAOSWW010000331         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29844_length_2636_cov_2.769469, whole genome shotgun sequence.
ACCESSION   DAOSWW010000331 DAOSWW010000000
VERSION     DAOSWW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29844_length_2636_cov_2.769469"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK442_07305"
     CDS             <1..598
                     /locus_tag="PK442_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO87492.1"
                     /translation="GHDRLEGITVVKVDENRRPIPGTDRFVACDTMLLAVGLIPENEL
                     SRMAGVEIHPVTGGPVLNEFYQTTRPSVFAAGNVVIVYDLADWVSQEGFAAGRNAALY
                     SAGTLRSSERRIAIKGGKNVRLFSPQFITGCCDATVYFRVTHPIEQRCRVFAEHGLFE
                     QKHRYVRPGEMNEVHLKGAALQALPESVAEITIDAEEV"
     gene            604..987
                     /locus_tag="PK442_07310"
     CDS             604..987
                     /locus_tag="PK442_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOO87493.1"
                     /translation="MEERLLNMICVGCPVGCDIKVAVEDTKVLSVEGNNCPRALEFAK
                     AEVANPTRVFATTVRVSGGKLPVCPVRSRQAVPKNRLFDISREVARLVVPAPVEVGQV
                     ILPDACGTGVDIVASRDLKTEEESA"
     gene            987..1244
                     /locus_tag="PK442_07315"
     CDS             987..1244
                     /locus_tag="PK442_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOO87494.1"
                     /translation="MEKQVVVRNPHGLHARPAAKFVQAASSVPEDVIIEKNGKTANAK
                     SILSIMSLGVSKGDTVTLTVAGESGEALLQTLEGILVSEAE"
     gene            1368..2378
                     /locus_tag="PK442_07320"
     CDS             1368..2378
                     /locus_tag="PK442_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HOO87495.1"
                     /translation="MKRRVFLSFAVLVCVIFAAAGAGMQAEASDYKLVLRLSHVFHPD
                     EQLTKSMDWVAERILKRTNGAIEIQTFPQGQIAVYKDGMEQVVRGADFISVEDPSYVG
                     DYVPDFIALVGPMLYNSYDEYVEIIKTDLVQDMIKRAEEKGIKILALEYVFGFRNVIT
                     NKVVKTPEDLKGVKLRTPGSQLFIQTLTAMGATVTPLPWAETLPGVQQGIVDGIEGSE
                     FTNIGTKVYELLKNVARTQHFLGTCGVYISTQVWEKIPEEYRAIIAEEFAKGALEMVE
                     IGKRDLGDVTAKLESYGVKFNEVDKKAFTEATKSIYETFPGLTPGIYDKLQAELEKIR
                     AK"
BASE COUNT          569 a          780 c          806 g          481 t
ORIGIN      
        1 cggtcatgac cgcctcgaag gaatcaccgt cgtcaaggtc gacgagaacc ggcgtcccat
       61 tccgggaacc gaccgtttcg tcgcttgcga cacgatgctg ctcgccgtcg gcctcatccc
      121 cgaaaacgag ctctcccgca tggcgggcgt cgaaatacac cccgtcacgg gggggccggt
      181 tctcaacgag ttctatcaga cgacccggcc ttcggttttc gccgccggaa acgtcgtcat
      241 cgtgtacgac ctcgccgact gggtcagcca ggaagggttc gccgcagggc gcaacgcggc
      301 cctgtactcc gccggaacgc tccgcagttc ggagcggcgg atcgccatca agggagggaa
      361 gaacgtccgg ctcttctcgc cgcagttcat caccggatgc tgcgacgcca ccgtctactt
      421 ccgcgtaacg cacccgatcg agcagcgctg ccgggtcttc gccgaacacg gactctttga
      481 acagaagcat cgctacgtcc ggcccggcga gatgaacgaa gtccacctca agggcgccgc
      541 actgcaggcc ctgcccgagt ccgtcgcgga aatcaccatc gacgccgagg aggtctgatc
      601 gccatggaag aacgtctgct gaacatgatc tgcgttggct gccccgtcgg gtgcgacatc
      661 aaagttgcgg tcgaagatac gaaggttctc tccgtcgagg ggaacaactg cccgagggcg
      721 cttgagtttg caaaggccga ggtggccaac ccgacgagag tcttcgcgac gacggtgcgc
      781 gtttccgggg gaaaactgcc cgtctgcccc gtcaggagcc gccaggcagt gccgaaaaac
      841 cgtctgttcg atatctccag ggaggtagcg cgcctcgttg ttcccgctcc cgtagaagtc
      901 ggacaggtta ttctccccga cgcctgcggg acgggcgtcg acattgtcgc aagccgcgac
      961 cttaagaccg aggaggagtc cgcataatgg aaaaacaggt cgttgtccgc aatccccacg
     1021 ggctgcacgc ccgccccgca gcaaagttcg tccaggcggc gtcgtccgtc ccggaggacg
     1081 tgatcatcga aaagaacggg aagactgcga acgcgaagag cattctctct attatgtccc
     1141 tcggcgtctc caagggagac acagtcaccc tgaccgtcgc cggagagagc ggcgaagccc
     1201 tgctgcagac cctcgaagga attttggtga gcgaggcgga gtagtttctg agaaaatcga
     1261 ttgacagaag cccggaaaga cgatagatat gaatacgggc ggttcgcgcc ggttgcggcg
     1321 gactgcccgg aggaagcgtc ctatcatact gaaggagagt gttgcgaatg aaacggagag
     1381 tgtttttgag tttcgctgtg ctggtgtgcg tcatctttgc ggcggcaggg gccggaatgc
     1441 aggcggaagc ctccgactac aagctggtgc tgcgtctgag ccacgtcttc catccggacg
     1501 agcagcttac caagtccatg gactgggtgg cggagcggat tctcaagagg acgaacggcg
     1561 ccatcgagat ccagacgttc ccccaggggc agatcgccgt ctacaaggac ggcatggagc
     1621 aggttgtccg aggagccgac ttcatctccg tcgaggaccc gtcctacgtc ggcgactatg
     1681 ttcccgattt catcgcgctg gtcggcccga tgctctacaa ctcctacgac gagtacgtcg
     1741 agatcatcaa gaccgatctg gtccaggaca tgatcaagag ggccgaggag aaggggatca
     1801 agattcttgc gcttgaatac gtcttcggct tccgcaacgt cattaccaac aaggtcgtca
     1861 agacgcccga agacttgaaa ggcgtgaagc tgcgcacccc gggaagccag ctcttcatcc
     1921 agacgctcac agcgatgggc gcgaccgtga ccccgctccc ctgggccgag actcttccgg
     1981 gagtgcagca gggaatcgtc gacggtatcg aaggctccga gttcaccaac atcggcacca
     2041 aggtgtacga gctgctgaag aacgtcgccc gcacccagca cttcctcggc acttgcggcg
     2101 tctacatctc cacgcaggta tgggagaaga tccccgagga gtaccgggcg attatcgccg
     2161 aggagttcgc gaagggcgcg cttgaaatgg tggagatcgg caagagagat ctcggcgatg
     2221 tcactgcgaa gcttgagtcg tacggcgtga agttcaacga ggtcgacaag aaggccttta
     2281 ccgaggcgac gaagagcatc tacgagacgt tccccggcct gacgcccggc atctacgaca
     2341 agctgcaggc ggagctggag aagatccggg cgaagtagtc gccggatttc cttcacgcga
     2401 atgaaataaa cgtacggcgc ggcagggaga gggaacgtgg tttctctccc tgccgctgat
     2461 tcactcgacg agaggttatc gaccatgaaa aaaatcgtac atacactctt cagctatgcg
     2521 gaggaaatcg tcagcgcatt cttcatcagc attaccgtct gcatggtcat cgtcaacgtc
     2581 tttttccgtt acgtgctcaa catggggatc ttctggtccg aagaggtcgc gaccat
//
LOCUS       DAOSWW010000332         2632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29901_length_2632_cov_3.014358, whole genome shotgun sequence.
ACCESSION   DAOSWW010000332 DAOSWW010000000
VERSION     DAOSWW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29901_length_2632_cov_3.014358"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..2014)
                     /locus_tag="PK442_07325"
     CDS             complement(101..2014)
                     /locus_tag="PK442_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO87496.1"
                     /translation="MKERFFPLFPDGRRGVSIAAKLALMIVAGVGAVFLAIVFYGYST
                     SREALEEEFQGRMENLGRAAESQFLQIPMVAEAVTRDLATMITLFEPPPEKMPEMMKE
                     LLASHSQVTGLYVGFSKQATNPLFRNFCPLVFRQGNSIQYKNLAGEMDWTVQDWYQLP
                     LQMGKPLWTEPFFADERAESMMISYGIPLYDANWRYLASIGASLELNWLADIISTMEV
                     GGHGYVFLVTSNGAFVSHPRRELLMRETLFTVAEMENRPDLIPLGHRMIRGEKGVASL
                     YDPLRGDLLVFFRPVGNIGWSLGIVFPEEEVLDDIIHLRRVQTFLGLGGVFAMLLMVF
                     FISNRISGPIRKLKDATMLLASGDLHAPLPPVRGRDEVALLTGSFASMRENLLLHLER
                     LKTETAARERIASELDIARSIQMSLVPRTFPPFPKDGRIDLFAKLEPAREVGGDFYDF
                     FHTDEDHLLLVVGDVSGKGIPAALFMAVTRSFVKALAISSGSCVPPGELMAAVNDRIA
                     EGNDACMFVTLCFVLVDLRDGSFSYACGGHPFPRLLGGDAALPLAPVKGPLVGAMEGM
                     FFEEGGGRLKEGEGIFLFTDGVTEAFDVDESLLSEGLMDRWLGEMRTLGSEARPGDAE
                     SDYSLRRRGGAVR"
     gene            complement(2011..>2632)
                     /locus_tag="PK442_07330"
     CDS             complement(2011..>2632)
                     /locus_tag="PK442_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO87497.1"
                     /translation="IRTIKDLNGRKMSLWGDHFSGGYKKAFSDNGIWPVIFPQFISVE
                     VFLRGGVDACSAMSYNEYLRILHTGRRPEDLTVISLRDEGYDFPEDGVYCTEETWRKN
                     PQLARNLREASLEGWRYAAEHPEEAVELILGEADKAGYIVNRLLLRRMLDTILPSIFP
                     EDGSTWTPGVLSRADYERTSEMMKSVFVDMTETAPYDVFFPGGDER"
BASE COUNT          537 a          825 c          790 g          480 t
ORIGIN      
        1 ggccttttcg gacgccctcc gttgtgcgcg ccatcctttc cgttccctct cctatacctt
       61 tttgttccct cccgtgtagc ggaagcagag cagcgtaata tcatcggact gctccgcccc
      121 tccggcgaag cgagtaatcc gattccgcat ctcccggacg cgcctcgctc ccgagcgtcc
      181 gcatctcgcc gagccagcga tccatgagcc cttcgcttaa cagcgactcg tcgacatcga
      241 acgcctccgt cacgccgtcg gtgaagagga agatgccctc cccttccttc agtcgtccgc
      301 ctccctcttc aaagaacatc ccctccatcg ccccgacgag cggccctttc accggagcta
      361 acggcagcgc agcgtcgccg cccagcagtc tggggaaggg atgccctccg caggcgtagc
      421 tgaacgaacc gtcccgaaga tccacgagga cgaagcagag cgtgacgaac atgcaggcgt
      481 cgttcccctc ggcgatgcgg tcgttgacgg cggccatcag ctctccggga ggaacgcagc
      541 ttccggagga gatggcaagc gccttgacga agctccgcgt cacggccatg aagagcgcgg
      601 cggggatccc cttgccggag acgtcgccga cgacgagcag cagatgatct tcatccgtgt
      661 ggaagaagtc gtaaaaatcg ccgccgactt cgcgcgccgg ttcgagcttg gcgaagagat
      721 cgatgcggcc gtccttcggg aacgggggga aggtgcgcgg aacaaggctc atctgaatgg
      781 accgggcgat gtccagctcg ctggcgatgc gctcgcgcgc cgcggtctcg gtcttcagac
      841 gttcaaggtg aagcagaaga ttctcccgca tggaggcgaa cgagccggtg agcagcgcga
      901 cctcgtcgcg gcccctcacg ggagggagcg gcgcgtggag atcgccggag gcgaggagca
      961 tggtggcgtc tttcagtttt cggatcggcc cgctgattct gttcgagatg aaaaaaacca
     1021 tcagcagcat ggcgaatact ccccccagac cgaggaaagt ctgcacccgg cgcagatgga
     1081 taatgtcgtc cagaacctct tcttcgggaa agacgatgcc gagcgaccag ccgatatttc
     1141 ccaccggacg gaaaaagacc aggaggtcgc cgcggagggg atcgtacagg gacgctaccc
     1201 ccttctcgcc ccgtatcatt ctgtggccga gagggatgag gtcggggcgg ttctccatct
     1261 cggccacggt gaagagcgtt tcgcgcatca gcagctcccg ccgcggatgg gagacaaacg
     1321 cgccgttgga cgtcacaaga aagacgtagc cgtgacctcc gacctccatc gtggagatga
     1381 tgtcggccag ccagttgagt tcgagcgacg cgccgataga ggccaaataa cgccagttcg
     1441 cgtcgtagag gggaataccg taactgatca tcatcgattc ggcgcgttcg tcggcgaaga
     1501 acggctccgt ccacaaaggc ttgcccatct gcaacggaag ctggtaccaa tcctgcaccg
     1561 tccagtccat ttcgccggca aggttcttat actgaatgga attcccctgg cgaaaaacca
     1621 acggacagaa attgcgaaag agcggattcg tcgcctgctt tgaaaagccg acgtacagcc
     1681 cggtgacctg cgaatgggac gcaagaagct ccttcatcat ctccggcatc ttctccggag
     1741 gcggctcgaa gagggtgatc atggtagcaa gatcgcgagt cacggcctcc gccaccatgg
     1801 gaatctggag gaactgactc tctgcggccc tgccgaggtt ctccatccgc ccctggaact
     1861 cctcctccag cgcttcccgc gatgtggagt agccgtagaa gacgatggcg aggaaaacgg
     1921 cgccgacgcc cgcgacaatc atcagcgcca gcttcgccgc tatggagacg cctctgcgtc
     1981 cgtcgggaaa gagagggaaa aaacgctcct tcatcgctcg tcacccccgg ggaaaaagac
     2041 gtcgtaaggg gctgtctccg tcatatcgac aaagacgctt ttcatcattt cggaggtgcg
     2101 ctcgtagtcg gcgcgagaca gaacgccggg cgtccatgtg gagccgtcct ccgggaagat
     2161 cgaggggagt atcgtgtcga gcatccgccg cagcagaagc cggttcacga tatacccggc
     2221 tttatcggcc tctccgagaa tcagctcaac ggcctcctca gggtgctcgg cggcgtagcg
     2281 ccacccttcg agggaggcct cgcggaggtt gcgggccagt tgcggattct ttctccacgt
     2341 ctcctccgta cagtagacgc cgtcctcggg gaagtcgtac ccttcgtccc ggagcgagat
     2401 caccgtcaga tcctccgggc ggcgtcctgt gtgcagaatg cgcagatatt cgttgtagct
     2461 catcgcggaa caggcgtcca cccctccgcg caggaagacc tccacggaga tgaactgagg
     2521 aaagatcacc ggccagatgc cgttgtcgct gaaagccttt ttatatccgc cggagaaatg
     2581 gtcgccccac aggctcatct tccgtccgtt cagatctttg atcgtgcgga ta
//
LOCUS       DAOSWW010000333         2631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_29920_length_2631_cov_3.273292, whole genome shotgun sequence.
ACCESSION   DAOSWW010000333 DAOSWW010000000
VERSION     DAOSWW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29920_length_2631_cov_3.273292"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PK442_07335"
     CDS             complement(<1..630)
                     /locus_tag="PK442_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87498.1"
                     /translation="MEQNFEEARSPVDPDAQVSEDYPPSFPEQEAKEPKRTLIDSLPS
                     ESVQELCVLFGCTREALEAILSTSSGDPAHIISLVQSLSPAFIAVKLLFETRKRGELG
                     GAACIVARGSSGEFLDQTLWVSSRELPATFSINADWEAVRNGIQKMGNNPDRSLFPKV
                     LKLLRDVFSPTAVNALFSSPSAKERTIALLEKAFIELYHSEVVIEMKTET"
     gene            complement(657..1151)
                     /locus_tag="PK442_07340"
     CDS             complement(657..1151)
                     /locus_tag="PK442_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOO87499.1"
                     /translation="MEKAHHVGMADLIVVSSPEMLMTLGLGSCIGLVLYDQTAKVAGM
                     VHIMLPDSRGAGAKAAEKPGKFADTAVPALINAVCAKGALRTRLKAKLAGGSQMFALP
                     GAETDFLAVGSRNAKETIDLLKKTGIPLVNSDVGGNKGRTVEFSTVTWMLTVKVLGKG
                     KTEI"
     gene            complement(1155..1781)
                     /locus_tag="PK442_07345"
     CDS             complement(1155..1781)
                     /locus_tag="PK442_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOO87500.1"
                     /translation="MDMENFSNMQLDAMREVGNIGAGNAATALSVMLSRLVDMDVPKA
                     ELVSIYELAEYYGDPLKPVSAVFVRSEGEFTCSLIFFQDEEDAQSLVDLLISQQMAGM
                     ALDDIPPEMRDSALTEVGNIILSSFLNAINVLMGGTHSISVPGVAHDMMGSILQVIAS
                     IFGQTGEYALIVDTTLKVGDIEKNVSGKVVMLPDPGSLEILFRKLQVL"
     gene            complement(1810..>2631)
                     /locus_tag="PK442_07350"
     CDS             complement(1810..>2631)
                     /locus_tag="PK442_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO87501.1"
                     /translation="MVHIIRNSIDHGIEKTEDRKKAGKPSKGIIKISAYQEGSGVIIE
                     VTDDGKGIDAAKVRAKALANGVITEEESAAMSDEEVVHLIFLPGFSMAEKITDVSGRG
                     VGMDAVKTKVESLGGQFDISTELGVGTRVFVRLPLTLAIVLALLIKVGEETYALPLEN
                     VEETILVKKENVKTVHGTPATLLRGEVLTLCDLSKMLHTPRASGEESDEYPVVVVKTG
                     KTRVGFIVDELIGQQDIVIKSLGRFLSKIKGIAGATILGDGNVALILDVAGLHGR"
BASE COUNT          547 a          790 c          709 g          585 t
ORIGIN      
        1 cgtctcggtt ttcatttcga tgacaacttc actgtggtag agttcgataa aggccttttc
       61 aagcaaggcg atggtcctct ccttggccga cggcgaggag aaaagagcgt tgactgcggt
      121 cgggctaaaa acatcccgca gaagcttgag caccttggga aagaggcttc tatccgggtt
      181 atttcccatc ttttgaatgc cgtttcgaac agcttcccaa tcggcgttga tactgaaggt
      241 ggcgggaagt tccctggaac tgacccataa cgtctggtcg agaaattccc cggaacttcc
      301 cctggcgacg atgcatgccg caccgccaag ttcccctctt tttcgggtct cgaaaagtag
      361 tttgacggcg ataaaggccg gggagagaga ctgcaccagg gagataatat gggcaggatc
      421 gcccgaagag gtggagagga tggcctcgag cgcctctcgg gtgcaaccga agaggacgca
      481 cagctcctgc accgactcgg aaggaagcga atcgataagc gtgcgcttcg gctctttcgc
      541 ttcctgctcc ggaaaagaag gaggatagtc ttcagaaacc tgagcgtcag ggtccacggg
      601 ggaccgggct tcctcaaaat tctgttccat gcgctcctct cccttcgaga attccgtcaa
      661 atctcggttt ttccctttcc gagaaccttg acggtgagca tccacgtcac cgtggaaaat
      721 tcaacggtac gccccttgtt ccctcccaca tcggagttca cgagaggaat acccgtcttt
      781 ttcagcagat cgatcgtctc ctttgcattc cggcttccga cagcaagaaa atccgtctcg
      841 gcgcccggaa gagcaaacat ctgcgaaccg ccggcaagct ttgccttcag ccgcgtgcga
      901 agcgctccct tcgcgcacac cgcgttgatc aacgccggca ccgccgtatc ggcgaacttc
      961 cccggttttt ccgccgcctt cgctccggcg ccccggctgt cgggcagcat gatatgcacc
     1021 atgccggcga cctttgccgt ctggtcgtaa aggacaagac cgatgcacga acccagtcca
     1081 agcgtcatca acatctctgg ggaggagaca acgatgagat ccgccattcc cacatgatgg
     1141 gctttttcca tggttcagag cacctgcagc tttctgaaga ggatttcaag cgaccctggg
     1201 tcaggaagca tgacaacctt tccggagaca tttttttcga tatcgccgac cttgagcgtc
     1261 gtatcgacga taagggcgta ctcgcccgtc tgcccgaaga tggaggcgat cacctggagt
     1321 atggacccca tcatgtcgtg cgcaactccg ggaacggaga tgctgtgcgt tccccccatg
     1381 aggacgttga tggcgttcag gaaagagctc aggatgatat tcccgacctc ggtgagcgcg
     1441 ctgtcccgca tctccggcgg aatatcgtca agcgccatac cggccatctg ctggcttatt
     1501 aagagatcca cgaggctttg cgcgtcctcc tcgtcctgaa agaaaataag actgcacgta
     1561 aactcccctt ccgagcggac aaaaacggcg gaaaccggct tgaggggatc cccgtaatac
     1621 tccgccagtt cgtagatcga gaccagttcc gcttttggaa catccatatc cactaagcgg
     1681 gagagcatga cggagagtgc ggtcgcggcg tttcccgcac cgatgttgcc gacttcgcgc
     1741 atggcatcca gctgcatgtt gctgaaattt tccatatcca tgaggtatgc cgcgtccttc
     1801 cttctgtatt tatctgccgt gcagaccggc cacgtccaga atgagcgcca cgttgccgtc
     1861 gcccaaaatg gtggcgcccg caatcccctt gatcttcgag aggaaccggc cgagcgactt
     1921 gatgacgata tcctgctggc cgatcagctc gtcgacaatg aagccgactc gcgtcttccc
     1981 tgttttgacg acgaccacgg ggtactcgtc gctctcctcg ccggaggctc gcggcgtatg
     2041 gagcattttc gaaagatcgc agagtgtgag cacttctccc cgaagcagcg tggcaggagt
     2101 accgtggacc gtcttgacgt tttccttctt cacgaggatg gtctcctcga cgttctccag
     2161 gggcagcgcg tacgtctcct caccaacctt gatcaaaagc gcgaggacga tcgcaagcgt
     2221 caatggcagt ctgacgaaaa cgcgcgtacc gacgcccagc tcggtcgaaa tatcgaactg
     2281 cccgccgagc gattcaacct tcgtcttgac cgcgtccatt cccacgcccc tgccggagac
     2341 atccgtgatc ttctccgcca tgctgaaacc gggaaggaag ataaggtgga caacctcctc
     2401 gtcgctcatg gcggcagact cttcttcggt gatgacgccg ttcgcaagcg ccttcgcacg
     2461 gaccttggcg gcgtcgatcc ccttgccgtc gtcggtgacc tcgatgataa ccccgctccc
     2521 ctcctggtac gcggatatct tgatgatacc cttggagggc ttgcctgctt tcttacggtc
     2581 ctctgtcttt tctataccgt ggtcgatgga gtttctgatg atatgcacca t
//
LOCUS       DAOSWW010000334         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30014_length_2626_cov_3.827694, whole genome shotgun sequence.
ACCESSION   DAOSWW010000334 DAOSWW010000000
VERSION     DAOSWW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30014_length_2626_cov_3.827694"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2216)
                     /locus_tag="PK442_07355"
     CDS             complement(<1..2216)
                     /locus_tag="PK442_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO87502.1"
                     /translation="MKISEFFVQNFGIFSGSGTSLSKGLTIFHGENESGKTTLMNFFR
                     RILFPREKYSRTRGNIYEPLSGARHGGSARVRMEDGREFVLTLDGKKNFVLPASGGSA
                     APLPSNFFSIGKDVYESVFAMGLNDMQSMESLNKADIAARFFSAGSGLGSASLPRLLS
                     SLEERGNELYRPGLAKGASAVNRLLASMKETDERIRELRMASGSWGERRDALDSAERA
                     LEQRREELAELQRRLSFLELLEKGLAPRVALGEIERSLGELSALPPFPEGGLDKLERL
                     KDERKRLEASCVRLESEIRAKEEEAAAPASDPLLLCTEYETEIRALEQEGEQLRAALA
                     RKNLLERELLASRRSFLRNIEDLCPWWTEEHLMSADISADAIAFARRTAERKEVLERK
                     RGEGEKSLAQWNRLREDRRSEAASLEKEMGVVEKRAKRASERWGLITRLRSVFTELCG
                     QEDELNSLEEARSTLLAERDRALEQEPIPPGIMVVLISAFLLLVGAGAALQAFLASDY
                     LWAFGSATLFMASLLAWFAHKDLSKKYATARGQWVKRLEDLDSRLEETTLLVESQKTR
                     VDRLRTQREELGSSLGINAPRFESAMEHLLAEGEGDNSAQERYTFLSERNRQMATVLA
                     RMDAEGEAMEQEVARTVKELETLNEAWSRWLTEHRFDRNLAPRDMEGIVPRILQLRSE
                     EASLAARAAEGRELDDAVEAIRKRIQELAGRLRPSFEALGEPAPSAADPASIRAFST"
     gene            complement(2213..>2626)
                     /locus_tag="PK442_07360"
     CDS             complement(2213..>2626)
                     /locus_tag="PK442_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87503.1"
                     /translation="RASVGGRGKLSALLRRPGFLSGPGGLVESLNQGEEGRSDFVYIE
                     SVVDDTASPFELEALSSGNHFVGDFLKEVSAFERRGNLREGLMEILSARGVREKLPRE
                     VFDRVDALPDDEILSLLRRGTTLALAGLLEGEDDE"
BASE COUNT          431 a          936 c          795 g          464 t
ORIGIN      
        1 gtcgaaaagg cgcggatcga cgcgggatct gccgccgagg gcgcaggttc gccaagggcc
       61 tcgaaggagg gcctgagacg tcctgcgagc tcctgaatcc gtttccggat ggcttcgacg
      121 gcgtcgtcca gctccctccc ctcggccgcc cgcgcggcaa gagaggcctc ctccgagcgc
      181 agctgaagaa tcctcgggac gatcccctcc atgtcgcggg gggcaaggtt acggtcgaac
      241 ctgtgttcgg tcagccagcg gctccacgcc tcgttcagcg tctccagctc cttgacggta
      301 cgcgcgacct cctgttccat ggcctcgcct tcggcgtcca tgcgcgcaag aacggtcgcc
      361 atctgtctgt tccgttcgct gaggaaggtg tagcgctcct gcgcgctgtt gtccccctcg
      421 ccctcggcga ggaggtgctc catcgcgctc tcgaagcggg gcgcgttgat gccgagactg
      481 ctccccagct cttccctctg cgtccgcagg cgatcgaccc tcgtcttctg gctctcgacg
      541 agcaaggtcg tctcctcgag cctggagtcg agatcctcca gccgtttcac ccactgaccc
      601 ctggcagtcg cgtacttctt cgacagatcc ttgtgcgcga accacgccag cagcgaggcc
      661 atgaagagcg tcgccgagcc gaaggcccac aggtagtccg acgccaggaa ggcctggagc
      721 gcggcgcccg cgccgaccag caggaggaat gcggagatca ggacgaccat gattcccggc
      781 ggaatcggct cctgttcgag ggcgcggtcc cgctccgcca agagcgtcga acgcgcctcc
      841 tcaagggagt tcagttcgtc ctcctggccg cagagctcgg tgaagacgct ccggaggcgc
      901 gtgatgagcc cccagcgttc ggaggcccgc ttcgcccgct tctcgacgac gcccatctcc
      961 ttttccagcg aggcagcctc gcttctgcgg tcctcgcgaa ggcggttcca ctgggcgagc
     1021 gacttctctc cctcgccccg cttccgttcg agcacctcct tgcgctccgc agtcctgcgg
     1081 gcgaaggcga tcgcatccgc ggagatgtcc gcgctcatca ggtgctcctc cgtccaccac
     1141 ggacagagat cctcgatatt gcggaggaag ctcctgcgcg aggccagcag ttcgcgctcc
     1201 agcagattct tgcgtgcaag cgcggcgcgc agctgctccc cctcctgctc cagcgcgcgg
     1261 atttccgttt cgtactccgt gcagagaagc agcgggtcgg aagccggcgc cgcggcctcc
     1321 tcctccttcg cgcgaatctc ggactcaagg cggacacagg aggcttcaag ccgcttccgc
     1381 tcgtctttca agcgctccag cttgtcaagc cctccctcag ggaacggagg aagggcggag
     1441 agttcgccga gggatcgctc gatctcgccg agcgcgacgc ggggcgccag ccccttctcc
     1501 agaagctcca gaaaggagag ccgccgttgc agttccgcaa gttcctcgcg ccgctgttcc
     1561 agcgcgcgct ccgcgctgtc cagcgcatcg cggcgctcac cccacgaacc gctcgccatg
     1621 cgaagctcgc ggatccgctc gtccgtctcc ttcatcgacg ccagcaggcg gttcacggcc
     1681 gaggcgccct tggcgagtcc gggccggtag agctcgttcc cccgctcctc caatgaggag
     1741 agcagcctgg gaagcgatgc cgaacccagg cccgaaccgg cggagaagaa gcgcgccgcg
     1801 atgtcggcct tgttcagcga ttccatcgac tgcatgtcgt tcagccccat cgcgaagacg
     1861 ctctcgtaga cgtccttgcc gatggagaag aagttcgacg gaagcggcgc ggcgcttcct
     1921 cccgacgccg gaaggacgaa gttcttcttc ccgtcgagcg tgaggacgaa ctccctgccg
     1981 tcctccatgc ggaccctggc ggaaccgccg tgccgcgcgc cgctcagcgg ctcgtagatg
     2041 ttccctcggg ttcgcgagta cttctccctc gggaaaagaa tccgccggaa gaagttcatc
     2101 agcgtcgtct ttccgctctc gttctcgccg tgaaagatcg tcagcccctt ggagagagag
     2161 gttcccgacc cggaaaaaat accgaagttc tgtacaaaaa actcgctgat cttcattcgt
     2221 cgtcctcccc ctcaagcagt cccgcaagcg caagcgtcgt ccctctgcgc agcagcgaga
     2281 ggatctcgtc gtcggggagc gcatccaccc tgtcgaacac ctcgcggggg agcttctccc
     2341 tgacgcccct cgcggagagt atctccatca acccttccct gagattgccg cggcgctcga
     2401 aggccgagac ttccttgagg aagtcgccga cgaaatggtt ccccgaggac agcgcttcga
     2461 gctcgaacgg ggacgcggtg tcgtcgacga cgctttcgat gtagacaaag tcgctccgcc
     2521 cctcctctcc ctgattcagg ctctccacaa gtccaccggg accggagagg aacccgggac
     2581 ggcgcagaag cgcgctcagc ttgccgcgcc cgccgaccga cgcccg
//
LOCUS       DAOSWW010000335         2625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30032_length_2625_cov_4.100778, whole genome shotgun sequence.
ACCESSION   DAOSWW010000335 DAOSWW010000000
VERSION     DAOSWW010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30032_length_2625_cov_4.100778"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PK442_07365"
     CDS             complement(<1..659)
                     /locus_tag="PK442_07365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO87504.1"
                     /translation="MVEVTRRCDLRCPVCFASAGEGEDPPFEHMAGIFKKIAARAPGS
                     IVQISGGEPAMRDDLPELVAEAAGMNFAGVQLNTNGLRLASEPGFARTLKEHGLSWVY
                     LQFDGTDDAVYERLRGRPLFDLKRRAVERCAEAQLGVVLVATVRRGTNDHLLGDIIRF
                     GTAHAPTVRGVHFQPMAFFGRYPEAPKDGDRTTLADVLRSVCEQYGFFRMEDFSPCGG
                     L"
     gene            complement(666..923)
                     /locus_tag="PK442_07370"
     CDS             complement(666..923)
                     /locus_tag="PK442_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87505.1"
                     /translation="MSFPKRTRSLCPVCMKPVDAVYQPEGRDIFLEKQCPEHGRFRTI
                     VWRGPLSLDEWSGGEIPEHPFTPSSRCPLDCGACEAHEAFG"
     gene            complement(920..1378)
                     /locus_tag="PK442_07375"
     CDS             complement(920..1378)
                     /locus_tag="PK442_07375"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOO87506.1"
                     /translation="MDVFDLDDLQMEMLSLEGKGYHCSQILILLALRQTGGERNDSLI
                     RSMGGLSLGLGHSNGTCGALLGGACLLGFYAGKGNDGETEHPMFRMMVRRLTEWFRDD
                     LCASGSVLCGDILNEHGRARCPQLVRSVWGKAMELLLENDIDPTEGRPEE"
     gene            complement(1378..1593)
                     /locus_tag="PK442_07380"
     CDS             complement(1378..1593)
                     /locus_tag="PK442_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOO87507.1"
                     /translation="MKELIKSFEKYTKTECGACGRFLELQKTEITYLGSVFTLDLPVC
                     PSCGTVFVPEELATGKMLEVEKILEDK"
     gene            complement(1590..>2625)
                     /locus_tag="PK442_07385"
     CDS             complement(1590..>2625)
                     /locus_tag="PK442_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOO87508.1"
                     /translation="AGCNPAQTTALYAFLRDRLDGGVGFWLRCCGAPADWAGRDEELR
                     REAAKITEEWESMGSPVVVAACTSCLQTFRKATPSIKAVSLWEILEGTELPEGALPLE
                     QTLAVADPCTAAQAPEIRNAVRNVASRTGVRFEEPSLSGEFSFCCGFGGLQESANPTL
                     AALSAKTRGEESSLDFLVYCAMCRNLFAQAGKPAVHLLDILFPQGDEDPAGRPAIGYS
                     EQQENRVGLVRALRASLWKEEDIVNEPHESIRLVIPPDVEKTLAKRRILADTVRRAIW
                     SAERSGRKMKQINGALVASLRPASVTYWVEYAPKGDGSFEVRNGWSHRMFVAGAAENL
                     VPDASGGDGA"
BASE COUNT          452 a          829 c          855 g          489 t
ORIGIN      
        1 tgcagaccgc cgcaagggga gaagtcctcc atgcggaaga agccgtactg ttcgcacacg
       61 ctcctcagta cgtcggcgag cgttgttcgg tcgccgtcct tcggggcctc ggggtatctg
      121 ccgaagaagg ccatcggctg aaagtggact ccgcggaccg tcggggcgtg cgccgtgccg
      181 aaccggatga tgtcgccgag caggtggtcg ttcgtgccgc gccgcacagt ggcgacgagg
      241 acaacgccga gctgcgcctc ggcgcatcgt tcgacggcgc gccgtttcag gtcgaaaaga
      301 gggcgtcccc tgagtctttc gtagacggcg tcgtcggttc cgtcgaattg aaggtacacc
      361 cacgaaaggc cgtgctcctt cagggtgcgt gcgaagccgg gttcggaggc gagacggaga
      421 ccgttcgtgt tgagctggac tcccgcgaag ttcatcccgg cagcctcggc gacgagttcg
      481 gggaggtcgt ccctcatggc gggctccccg ccggagatct gcacaatgct cccgggagcg
      541 cgcgcggcga tttttttgaa gatgccggcc atgtgctcaa acggaggatc ctctccttcc
      601 ccggcggagg cgaaacagac ggggcagcga aggtcgcagc gacgtgtgac ctcgaccacg
      661 aaagatcagc cgaaggcctc gtgcgcctcg catgcgccgc agtcaagggg gcagcgggat
      721 gagggggtga acgggtgttc cggaatttcg ccgccgctcc actcgtccag ggagagcggt
      781 cccctccaga cgatggtccg gaagcggccg tgttcagggc actgtttttc caggaaaata
      841 tcgcgcccct ccggttggta gacggcgtcg acgggcttca tgcagacggg gcagaggctg
      901 cgcgtgcgtt tcgggaagct cattcctccg gccgtccttc ggtcgggtcg atatcgttct
      961 ccaaaagcag ctccatcgcc ttgccccaga cggagcggac gagctgcgga cagcgggcgc
     1021 gtccgtgttc gttgagaatg tcgccgcaga ggacgcttcc ggaggcgcac aggtcgtccc
     1081 ggaaccactc cgtcagtcgg cggaccatca tccggaacat cgggtgctcc gtttcgccgt
     1141 cgttcccctt cccggcgtag aatccaagga ggcatgcgcc gccgagcagc gcgccgcacg
     1201 tgccgttgct gtgaccgagc ccgagggaga gccctcccat cgaacggatc agcgagtcgt
     1261 tgcgctctcc tcctgtttgg cgaagcgcca gcagtatgag aatctggctg cagtggtacc
     1321 ccttcccttc gagggagagc atctccatct gcaggtcgtc gagatcgaaa acatccatta
     1381 cttgtcctcc agaatcttct ccacttcgag catcttcccc gtggccagct cttcggggac
     1441 gaaaaccgtg ccgcaggatg ggcagaccgg taaatccagc gtgaagacgc tgccgagata
     1501 ggtgatctcg gtcttttgca actccagaaa acggccgcat gcgccgcatt cggtcttcgt
     1561 gtacttctcg aacgacttga tcagctcttt catgctccgt ctcctccgga agcgtcgggg
     1621 acaagattct ccgcggctcc ggcgacgaac attctgtggc tccacccgtt gcgcacctcg
     1681 aaagagccgt cgcctttggg agcgtactcc acccagtagg tgaccgacgc cgggcgcagc
     1741 gaagcgacca gcgcgccgtt gatctgtttc atcttgcgtc cgcttcgttc cgccgaccag
     1801 atggctctcc ggacggtatc ggcaagaatt cgccgcttcg ccagagtctt ttccacgtcg
     1861 ggcgggatga caagccggat ggattcatgc ggttcgttca cgatgtcttc ctccttccag
     1921 agagatgcgc gcagcgcgcg gaccagtccg acccggttct cctgctgttc ggagtagccg
     1981 atggctggac gcccggccgg gtcttcgtct ccctgcggaa agaggatatc cagcaggtgg
     2041 acggcgggtt tccccgcctg tgcgaagaga ttccggcaca tggcgcagta tacgagaaag
     2101 tcaagagaac tctcctcccc ccgggttttg gcggaaagag cggccagggt cgggttggcg
     2161 ctctcctgga gtccgccgaa gccgcagcag aaggagaatt ctcctgaaag tgaaggctct
     2221 tcgaatcgca cccccgttcg ggaggcgacg tttcgcacgg cgttcctgat ttcgggcgcc
     2281 tgtgcggcgg tgcaagggtc ggcgacggca agggtttgct cgagagggag cgccccctcc
     2341 ggaagctccg tgccctccag gatctcccac agtgagacgg ccttgattga cggcgtcgcc
     2401 ttgcggaagg tctggaggca gctcgtgcag gcggccacga cgaccgggga gcccatggac
     2461 tcccactctt ccgtgatctt cgcggcttcc cggcggagtt cctcgtcgcg ccccgcccag
     2521 tccgcaggag cgccgcagca gcggagccag aagccgacgc cgccgtccag acggtccctc
     2581 aaaaaagcgt agagggcggt cgtctgtgcg ggattgcagc ccgcc
//
LOCUS       DAOSWW010000336         2624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30054_length_2624_cov_2.808486, whole genome shotgun sequence.
ACCESSION   DAOSWW010000336 DAOSWW010000000
VERSION     DAOSWW010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30054_length_2624_cov_2.808486"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PK442_07390"
     CDS             complement(<1..652)
                     /locus_tag="PK442_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO87509.1"
                     /translation="MKQYAIGLVPGPVSVPEEFRRAYLENFGSADLEEEFFLLYEENE
                     SLLRKLLKTKNAVTIQSGEAMSVLWGALKSILLPGDRLLAVSNGVFGRGFGEMGMMLG
                     MEVRIVEAPDGEFPDLDSIRTAAAEFRPRMISAVHCETPSGILNPVAGVGEIARETGA
                     LFCVDYVASAGGADVRTDEWGIDIGLLGSQKALSLLPDLSIIAVSDRAWETAERTNY"
     gene            complement(763..2229)
                     /gene="xylB"
                     /locus_tag="PK442_07395"
     CDS             complement(763..2229)
                     /gene="xylB"
                     /locus_tag="PK442_07395"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOO87510.1"
                     /translation="MNVYLGLDIGTTNIKMLLVDERGSVVKSMSHPLELSAPRPAWAE
                     QDPEDWWNGVWTLLSSVPSDMNVLRIGLSGQMHTLVPVDSEGNVLRKAILWCDQRTSA
                     ECDEATAAVGGEEKIIEMTGNLIYPGFTLPKILWIRKNEPEVYSRISTCFIAKDYIGW
                     RLTGERAAEWSDASGSSCYDVSARRWRSDLLRTLGIDEAWLPSVKGSSDIRGKLRPEL
                     ASKLGWKQAEVVCGGADNAVAALGLGVCSPGDCMISIGTSGTVLAATESTSPDPTGRL
                     HFFNTVLPGQSYYMGCMLAAAASLNWFKDTMGKSLSWDEIEKGINEIPAGAEGTLWLP
                     YLNGERTPHRNPNARGVLYGLSSMSDERHVFRAVMEGITYGLRDSFELVKAATPVKRV
                     LLVGGGAKNATWRRMIAANVKAPVSVPLVDEGGAYGAAMLAALGGGVSLDEVKGWIKI
                     HETVEPDSEESAAYDRVYPQFRALYADLKERFDATAGM"
     gene            complement(2249..>2624)
                     /locus_tag="PK442_07400"
     CDS             complement(2249..>2624)
                     /locus_tag="PK442_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOO87511.1"
                     /translation="DQALPLLEVGDLLGLLVELLTAGLSLGTPTYDTYINSCGICHYF
                     QATRLDLFGNEEKIKAHVASILEKLRESPKAAEKERIYIHGEKEFERRKKSMEEGVYL
                     DPATWERLDEFADKFGMRRLEP"
BASE COUNT          429 a          831 c          820 g          544 t
ORIGIN      
        1 gatagtttgt ccgctccgca gtctcccatg cccggtcgct caccgcgata atcgagaggt
       61 ccggcagcag cgagagcgcc ttctggctcc cgagaagccc gatgtcgatt ccccattcat
      121 cggtgcggac gtccgcgcct cccgcgctcg ccacgtagtc gacgcaaaag agcgcgccgg
      181 tctccctcgc tatttcgccg acgcccgcca ccgggttcag aataccgctc ggcgtttcgc
      241 agtggacggc ggagatcatc ctgggacgga attcggccgc cgccgtccgt atcgagtcga
      301 gatcggggaa ttccccgtcc ggagcctcga cgatgcgcac ttccatgccg agcatcatcc
      361 ccatctcgcc gaatcctctg ccgaaaactc cgttcgagac ggcaagcagc cggtcgccgg
      421 gcagcaggat gctcttcaga gcgccccaga gcacggacat cgcctctccg ctctggatgg
      481 tcacggcgtt tttcgtcttc agaagttttc ggagcagcga ctcgttctcc tcgtagagga
      541 ggaagaactc ctcttccagg tcggcgctgc cgaagttctc caggtaggct ctccgaaact
      601 cctcgggaac cgatacgggc cccggtacga ggccgattgc gtactgtttc atgcgttgca
      661 tcctccgttt ccgactgatt gagtgtgtgt tccgtgaaga aaagaggagg cccgggagcc
      721 tcctccgtgt tgcttttttc tttttgagta tccgcggatt ggctacatgc ctgccgtggc
      781 gtcgaaacgc tccttcagat cggcgtagag cgcccgaaat tgcgggtaca ccctgtcgta
      841 cgccgcagat tcttcggagt ccggttcgac cgtttcgtgg attttgatcc accccttcac
      901 ctcgtccagg gaaacgccgc cgccgagcgc cgcgagcatc gccgcgccgt aggcccctcc
      961 ttcgtccacc agcggaaccg agacgggcgc cttgacgttg gcggcgatca tgcggcgcca
     1021 cgtggcgttc ttcgcgccgc cgccgacgag gagcacccgc ttcacagggg tggccgcctt
     1081 caccagctcg aaggagtcgc gcaggccgta ggtgatcccc tccatcacgg cgcggaagac
     1141 gtgccgctcg tccgacatcg aggagagccc gtagaggacg cccctcgcgt tcgggttccg
     1201 gtggggcgtc ctttcgccgt tgaggtaggg gagccagagc gttccctccg ctcccgcagg
     1261 tatttcgttg atgcctttct cgatttcgtc ccaggagagc gattttccca ttgtgtcctt
     1321 gaaccagttg agcgaggccg ccgctgcgag catgcacccc atgtagtagg actgcccggg
     1381 aaggacggtg ttgaagaagt gcagtcttcc cgtaggatcc ggagatgtgg actctgtggc
     1441 ggcgagcacc gttcccgaag tgccgatgga gatcatgcag tcgccgggcg agcagacgcc
     1501 gagcccgagc gcagcgaccg cgttgtccgc gccgccgcac acaacctcgg cctgtttcca
     1561 gcccagtttc gaggcaagtt ccgggcggag tttcccgcgt atgtccgagg agcccttcac
     1621 cgagggcagc cacgcctcgt cgatgccgag cgtgcgcagc aggtcgctcc gccagcgccg
     1681 ggcggagacg tcgtagcacg aacttccgga ggcgtccgac cactcggcgg cccgctctcc
     1741 ggtgagccgc cagccgatat agtccttggc gataaagcat gtactgatgc ggctgtagac
     1801 ttcgggttcg ttcttgcgga tccagaggat cttcggcaga gtgaatccgg ggtagatcag
     1861 gttcccggtc atctcaatga tcttttcctc tcctccgacg gcggcggtcg cctcgtcgca
     1921 ttcggcggac gttctctggt cgcaccagag gatcgccttg cgaagaacgt ttccctcgct
     1981 atcgacgggg acgagcgtat gcatctgccc cgagaggccg atgcggagga cgttcatgtc
     2041 ggaggggacg gaagagagga gcgtccatac gccgttccac cagtcttccg ggtcctgctc
     2101 ggcccaggcc gggcggggcg cggagagttc gagtgggtgc gacatgctct tgacgacgct
     2161 cccccgttcg tccacgagga gcatcttgat gttcgtggtg ccgatgtcca ggccaagata
     2221 gacgttcata cggtcccctc cctttggttt acggttcgag acggcgcata ccgaacttgt
     2281 cggcgaattc gtcgagacgc tcccatgttg ccgggtcgag gtagacgccc tcttccatgg
     2341 atttcttccg gcgctcgaac tctttctcgc cgtggatgta gatacgctcc ttttccgctg
     2401 ccttggggct ttcgcgcagc ttttccagga tggaggcgac gtgcgccttg atcttctcct
     2461 cgttgccgaa gaggtcgagt cgtgtggcct ggaagtagtg gcagattccg cagctgttga
     2521 tgtacgtgtc gtacgtcgga gttccgaggg agagccctgc ggtgagcagc tcgacgagca
     2581 ggccgagaag atcgcctacc tcgaggagtg gaagcgcctg atcg
//
LOCUS       DAOSWW010000337         2622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30086_length_2622_cov_3.322945, whole genome shotgun sequence.
ACCESSION   DAOSWW010000337 DAOSWW010000000
VERSION     DAOSWW010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30086_length_2622_cov_3.322945"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK442_07405"
     CDS             complement(<1..277)
                     /locus_tag="PK442_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOO87512.1"
                     /translation="MKRLGALIAVFILFSSLFATIGSAAEPAGYAATIAAAREIVWKT
                     LTTGGGSSATVAVMDGGKIVYSEQFGAAERATNRPADRETRFNIGSTS"
     gene            complement(297..>2622)
                     /locus_tag="PK442_07410"
     CDS             complement(297..>2622)
                     /locus_tag="PK442_07410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO87513.1"
                     /translation="GRREYGALVYLMDVRAAASALPGDEFRVRSSDFQYPFDERRPLL
                     LGPFAHDDGSFFAALVPLVEPVTRRVIAVFLLETDARNWRREPYRAALVPWGATILLL
                     MVLFLGYRFSPRLRDSRVFVYAEVLWVASMSLLLAGSCAWLVGAHEYRGRTKAFSMLS
                     DFGSSALIQSLQELRDCSIEGLGRFFESSQLVDAEEFAGFARHLIAIPHVDEVLWAPA
                     VPSLAAESGDLSEAGLLSSGDLTEGPSMPSSSSYALSFPVLYSVSADRRILPPGTDLR
                     SIPSLKHAIELSVRNGMKTASEAATSDVGSDGACRSVLFRPVRSAGGDLMGVVGVVLN
                     LEVLLNDTMLRDEGAQQHVMEVELKRLLPGGEERLLLSMPSGADALPRERNAESFHSL
                     RPFFVFGRAFVLETRSGEGFASMHPERMGKIVLFFGIAVSALITLLAELFLNWRRTLL
                     RFVTIKTVSLAQSEFRSRKLAHLYRSITEGVVITDAEGLIEECNDALEELTGFALEEM
                     QGRSLAIFSAREGQYDHEFLRSLGQNGVWQGEVWNRKRDGEVYPVWLTIAAVQDDSGQ
                     TTHYSGVMRHIGGIKSEQERLNRMAYYDFLTGLPNRALFTDRLEVAMARAKREGSMVA
                     LVFLDLDRFKEVNDAFGHEAGDALLIAVADRLSGAFREQDTVARLAGDEFVLLFEGLR
                     SDGELDPLHERLEALFQEPFTADGRSISMEASFGVAFFPEDGADVRSLLDIADGKMYA
                     EKTARRKRRGAASGSDEEENGRSGTFDGNAEEEG"
BASE COUNT          549 a          843 c          771 g          459 t
ORIGIN      
        1 tgctggtgga gccgatgttg aagcgcgtct ctctatccgc ggggcggttc gtcgcgcgct
       61 ccgccgcgcc gaactgctcg gagtagacga tcttccctcc atccatgacg gccacggtcg
      121 cggaacttcc tccgccggtc gtgagcgtct tccagacgat ctccctggcg gcggcgatgg
      181 tcgccgcgta tccggctggt tcggccgccg agccgatagt cgcgaacaac gaggagaaca
      241 ggatgaaaac tgcaataaga gcgccaagcc ttttcacgat tctttcaccc ttccctttac
      301 ccttcttctt cggcgtttcc gtcgaaagtc cctgaacggc cgttttcttc ttcgtcggat
      361 ccggaagccg ctcccctgcg ctttctgcgc gctgtttttt cagcgtacat ctttccgtcc
      421 gcaatgtcca gaagagaacg gacatctgct ccgtcctccg ggaaaaacgc aacgccgaag
      481 ctcgcctcca tcgaaatgga acgcccatcc gcagtaaacg gctcttgaaa aagcgcctcc
      541 aggcgctcat gcagcggatc gagttcacca tcggaacgca acccctcgaa gaggagcaca
      601 aattcgtccc ccgcaaggcg tgcaaccgtg tcctgttcgc ggaacgcgcc gctcaaacga
      661 tccgccaccg cgataagcag agcgtccccg gcttcgtgcc cgaaggcgtc gttgacctcc
      721 ttgaagcggt cgagatcaag aaagaccagc gcgaccatcg aaccctcgcg tttcgccctc
      781 gccatggcga cctccaggcg gtcggtgaag agcgcgcggt tgggcagccc ggtcaggaaa
      841 tcgtagtatg ccatcctgtt caggcgctcc tgctcgctct tgatgccgcc gatgtgccgc
      901 atcacgcccg agtagtgcgt cgtctgcccc gagtcgtcct gaaccgccgc gatggtgagc
      961 cagacagggt atacttctcc gtccctcttc cggttccaca cctcgccttg ccataccccg
     1021 ttctgcccga gcgagcgcag gaactcatga tcgtactgcc cctccctggc ggagaagatg
     1081 gcaagcgatc tcccctgcat ctcctcaagc gcgaagcccg tcagctcttc gagcgcgtcg
     1141 ttgcactcct cgatgaggcc ctccgcatcc gttatcacca ctccctccgt gatggagcgg
     1201 tagaggtgcg cgagcttgcg ggaacgaaat tcgctctgcg ccagagagac cgtcttgatc
     1261 gtcacgaagc ggagcagcgt ccgccgccag ttgagaaaga gctcggcgag cagcgtaata
     1321 agcgccgaga cggcgatacc gaagaagaga acgatcttcc ccattcgttc cgggtgcatc
     1381 gaggcaaatc cttccccgga gcgcgtctcc agcacaaaag cccgaccgaa cacgaagaag
     1441 gggcgcagag agtgaaaaga ctccgcgttc cgttcccttg gaagcgcatc cgctcctgat
     1501 ggcatagaga gcaacaggcg ctcttccccg ccgggcagga ggcgcttgag ttcgacctcc
     1561 atgacgtgct gttgcgcccc ctcgtcacgc agcatcgtat cgttcagcaa aacctccagg
     1621 ttgagcacga cgccgacaac gcccatcagg tcgcccccgg cgctccgcac aggacggaaa
     1681 aggacggaac ggcatgcccc gtcggaaccg acgtcgctcg tcgccgcttc ggaggcggtc
     1741 ttcatgccgt ttcgtacaga gagctcaatg gcatgtttta aagagggaat ggatcgcaga
     1801 tcggtccccg gaggcagtat cctccggtcg gcggataccg aatagagcac cgggaaggag
     1861 agcgcgtagg aagatgaaga aggcatggaa ggtccttccg tcaaatcccc ggaagagaga
     1921 agtccggctt cggacaggtc gccgctttcg gctgctaaag agggcacggc cggcgcccac
     1981 agcacttcgt caacatgggg tatggcgatc agatgtctgg cgaagcccgc aaattcttcg
     2041 gcgtcgacaa gctggctgct ctcgaaaaaa cgtcccaatc cttcgatgct gcaatcccgc
     2101 agctcctgga gcgactgtat caaggcgctg gagccgaagt cggagagcat ggagaacgcc
     2161 ttcgttcgcc cgcggtattc atgcgcgccc acgagccatg cgcacgatcc ggcgaggaga
     2221 agactcatcg aggcaaccca gagcacctcc gcgtacacga aaaccctcga gtcgcggagt
     2281 cggggggaga agcggtagcc taaaaaaaga accatgagga ggagtatcgt cgctccccac
     2341 ggaacaagcg ccgccctgta cggctcgcgc cgccagttcc gggcgtcggt ttcaagaagg
     2401 aatacggcga tgacacggcg cgtcaccggt tccaccaaag ggaccagcgc cgcaaaaaaa
     2461 gagccgtcgt cgtgagcgaa aggaccgagc agaagcggcc gtctttcgtc gaaagggtat
     2521 tggaagtcgg aggaccggac acggaattca tcgccgggga gcgcggaagc ggcggcgcgt
     2581 acatccatca ggtagacgag ggcgccgtac tcccgcctcc cg
//
LOCUS       DAOSWW010000338         2619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30152_length_2619_cov_3.572543, whole genome shotgun sequence.
ACCESSION   DAOSWW010000338 DAOSWW010000000
VERSION     DAOSWW010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30152_length_2619_cov_3.572543"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK442_07415"
     CDS             complement(<1..413)
                     /locus_tag="PK442_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOO87514.1"
                     /translation="MNTDFIKGVIPPIVTIVDENEKIDEKRMRRQVDFVIEGGVHGIL
                     AFGSNGEFYMLDEDEMERGLGIILDQTKKRVPVYFGMGAVKTRKCVQLARMAADMGAD
                     GVSVLQPMFLKPTEDELYLHFKTIAVAVPETPVLL"
     gene            complement(540..2051)
                     /gene="garD"
                     /locus_tag="PK442_07420"
     CDS             complement(540..2051)
                     /gene="garD"
                     /locus_tag="PK442_07420"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR03248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HOO87515.1"
                     /translation="MIPVIRVDERDNVAIPVADLPEGSEAGWGVVAREQIPQGHKIAL
                     EDIPEGGAVIRYGVALGHLLAGVPQGGLITEKMLRLPDSPDLDSLEFATDIDAELPEA
                     PVKSFEGFEVEGSLFAGTRNILGIMPTVQCVAGVLNGAVKRMRRELLPRFPHVDDIVA
                     LNHPYGCGVAIDAPEAVVPIRTLRNILRNPNFGGEIMVVGLGCEKLTADRLLEPEEIR
                     PGNVLMLQEHAGFVKMTDALMEMAERKLRRLEDRRRTTLPFSRLCVGMQCGGSDAFSG
                     ITANPAAGYASDLLVGAGATVLFSEVTEVRDGVEQLAARCVNSAVGRRLVEEMRWYDE
                     YLRAGGVDRSANPTPGNKKGGLANIVEKAMGSIAKSGRAPISEALAPGERPSAGGLVF
                     AATPASDMVCGTCQLASGMTLQVFMTGRGTPYGLAAAPVIKVSSRSNLKDMWSDLIDV
                     DAGVIASGGADIRTVGERLFREIVEVASGRTHPAAERYRLFNDICLFNPAPIT"
     gene            complement(2048..>2619)
                     /locus_tag="PK442_07425"
     CDS             complement(2048..>2619)
                     /locus_tag="PK442_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO87516.1"
                     /translation="PLLSLGIPGDSTTAVLVGAFMLQGVQVGPLFITNNLDLWHVILS
                     ALLLCNFLMFFVMFYPIKYIASVIKLPKYRIFPVIILLCVIGAYSSRNGNMFDVWTML
                     FFGLLGYFFVKIKLPIVPFLIGFILGKDLEKYFVDSIKGVGGSLSVFFTRPMGWVIWG
                     LIVASIAWAVYDSKREARRAAAGNGGAQA"
BASE COUNT          477 a          875 c          806 g          461 t
ORIGIN      
        1 tacagcagca ccggcgtctc cgggacggcc acggcgatgg ttttgaaatg gaggtacagc
       61 tcgtcctccg tcggcttcag gaacatcggc tgcagcacgg agacgccgtc cgctcccatg
      121 tccgcggcca tgcgggcgag ctggacgcac ttcctcgtct tgacggcgcc catgccgaag
      181 tagacgggga cgcgcttctt cgtctggtcc agaataatcc cgagcccccg ctccatctcg
      241 tcctcgtcca gcatgtagaa ctcgccgttg ctcccgaagg cgaggatacc gtggacgccg
      301 ccctcaatga cgaagtccac ctgccggcgc atcctctttt cgtcgatttt ttcgttttca
      361 tccactatgg tcactatggg ggggatgacc cccttgatga agtccgtgtt cacaggtacc
      421 agtcctttcg cgtcgcccgc cgttcaatac ggcgggagta tggtcttgca acattcgtcc
      481 ggggcggcgc gtcgttccgt tccgcgagcg ccccgccgga aatctcctga tggagcattc
      541 tacgtgatcg gggccgggtt gaaaaggcag atgtcgttga acagccggta gcgttcggcc
      601 gccgggtgcg ttcgcccgct tgcgacctcg acgatctcgc ggaagagccg ttcgcccacg
      661 gtccggatat ccgccccgcc ggacgcgatt actcccgcgt ccacatcgat caggtcgctc
      721 cacatgtcct tcaggttcga ccgcgaggag accttgatca caggcgcggc ggccagtccg
      781 tagggcgtcc ccctccccgt catgaacacc tggagcgtca tgcccgaggc aagctggcag
      841 gttccgcaga ccatgtcgct cgcgggcgtg gcggcgaaga cgagcccccc ggcggacggg
      901 cgctctccgg gggcaagcgc ctccgaaatc ggagctcgtc ccgacttggc gatcgagccc
      961 atcgccttct cgacgatgtt cgccagcccg cccttcttgt ttccgggagt ggggttggcg
     1021 ctgcggtcca cgccgcccgc acggaggtac tcgtcgtacc atcgcatctc ttcgacgagc
     1081 cgcctcccca cggcggagtt cacgcagcgc gcagcgagct gctccacgcc gtcccgcact
     1141 tcggtgacct cggaaaagag caccgtcgcg cccgccccga ccagaaggtc cgacgcgtac
     1201 ccggccgccg ggttcgcggt aatgccggaa aaggcgtcgc tccctccgca ctgcatcccg
     1261 acgcagaggc gagagaaggg cagcgtcgtc ctgcgcctgt cctccagcct gcggagcttc
     1321 cgctccgcca tctccatcag ggcgtcggtc atcttgacga acccggcgtg ctcctggagc
     1381 atcagcacat tcccggggcg gatctcctcc ggttcgagca gcctgtccgc ggtgagcttc
     1441 tcgcacccca gtccgacgac catgatctcc cctccgaagt tcgggttccg cagtatgttc
     1501 cgcagcgtcc tgatgggaac gacggcctcg ggggcgtcga tggcgacccc gcagccgtag
     1561 gggtggttca gcgccactat gtcgtccacg tgcgggaagc gcggaagcag ctccctccgc
     1621 atccgcttga ccgcgccgtt gagcacgccc gcgacgcact ggaccgtggg catgatgccg
     1681 agaatgttcc gcgtaccggc gaagagggag ccctcgacct cgaacccctc aaagctcttc
     1741 acgggcgcct cgggaagctc cgcgtcaatg tcggtggcga actcaagact atcgaggtcg
     1801 ggagagtcag gcagacgaag catcttctcg gtgatcagtc cgccctgcgg aacgcctgcg
     1861 agcagatgcc cgagcgccac gccataccgg atcaccgcgc caccctcggg gatgtcctcc
     1921 agagcgatct tgtgcccctg ggggatctgt tcacgggcga ccacacccca tccggcctcc
     1981 gaaccttcgg gaaggtcggc gacggggatg gcgacgttgt ccctctcgtc caccctgatg
     2041 accggtatca tgcctgcgcg cctccgtttc cggccgcggc gcgccgggcc tctcttttcg
     2101 agtcgtaaac cgcccacgcg atggaggcga cgatcaaccc ccagatcacc caccccatcg
     2161 ggcgcgtgaa aaagaccgag agggagccgc cgaccccctt gatggagtcg acgaagtact
     2221 tctccagatc cttcccgagg atgaagccga tgagaaaggg aacgatcggg agcttgatct
     2281 tcacgaagaa ataccccagg aggccgaaaa agagcatggt ccacacgtcg aacatgtttc
     2341 cgttccgcga agaatacgca ccgatgacgc agaggaggat gatcacgggg aatatcctgt
     2401 acttgggaag cttgatgacc gaagcgatgt acttgatcgg gtagaacatg acgaagaaca
     2461 tcaggaagtt gcacagcaaa agcgcggaga ggatgacgtg ccagaggtcg agattattcg
     2521 tgatgaagag cggaccgacc tgcactccct gaagcatgaa ggcgccgacg agcactgccg
     2581 tggtgctgtc cccggggatg ccgagggaga gcagcggaa
//
LOCUS       DAOSWW010000339         2616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30203_length_2616_cov_4.007029, whole genome shotgun sequence.
ACCESSION   DAOSWW010000339 DAOSWW010000000
VERSION     DAOSWW010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30203_length_2616_cov_4.007029"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /gene="rpsD"
                     /locus_tag="PK442_07430"
     CDS             complement(<1..378)
                     /gene="rpsD"
                     /locus_tag="PK442_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOO87517.1"
                     /translation="MSRYIGPACRLCKAEGTKLFLKGDRCYTAKCAITKKEGATVKGK
                     RGRPRTKVSEYGIRLREKQKLRRFYGMTELQFKRFFEIANKQSGQTGHNFLSLLERRL
                     DNVVYRLGFANSRKQARQLVLHGH"
     gene            complement(412..804)
                     /gene="rpsK"
                     /locus_tag="PK442_07435"
     CDS             complement(412..804)
                     /gene="rpsK"
                     /locus_tag="PK442_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOO87518.1"
                     /translation="MAKRTVRRGKKKERKNISYGVAHIYSTFNNTIVCVSDKGGNVLA
                     WASGGNVGFKGTRKSTPFAAQIAASQVAKAVQEHGVQEIDVVVKGPGPGRESAIRSLQ
                     AAGLQVNMIKDSTPVPHNGCRPPKRRRV"
     gene            complement(837..1214)
                     /gene="rpsM"
                     /locus_tag="PK442_07440"
     CDS             complement(837..1214)
                     /gene="rpsM"
                     /locus_tag="PK442_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOO87519.1"
                     /translation="MARIAGVDIPREKRVEIALTYIFGIGLPTSKKILAVTGVSPDTR
                     VKDLTEEESQKLRREIEDHYKVEGDLRREVSMNIKRLMDIGCYRGQRHKLGLPVRGQK
                     TKTNARTRKGPRRTVAGKKMATK"
     gene            complement(1221..1346)
                     /gene="rpmJ"
                     /locus_tag="PK442_07445"
     CDS             complement(1221..1346)
                     /gene="rpmJ"
                     /locus_tag="PK442_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOO87520.1"
                     /translation="MKVKPSVKPMCEFCRVIRRKGVVRIICSRNPRHKQRQGARR"
     gene            complement(1458..1679)
                     /gene="infA"
                     /locus_tag="PK442_07450"
     CDS             complement(1458..1679)
                     /gene="infA"
                     /locus_tag="PK442_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOO87521.1"
                     /translation="MSNKDEVIEVRGKVIEPLPNAMFKVELDNGHKILAHVSGKMRLH
                     FIRILPGDRVLVEISPYDLSRGRIIYRYK"
     gene            complement(1658..2083)
                     /locus_tag="PK442_07455"
     CDS             complement(1658..2083)
                     /locus_tag="PK442_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HOO87522.1"
                     /translation="MGMGLNELSDGGMCTIGQVVRSRKGKDAGRWYVVVGVAEDCGPS
                     RRTQGARVLVADGKKFTVGKPKAKNMIHLQRTRWVLDEIAHSILSKGNFDAGRFQALL
                     TGLANKNSIGGSVGCDPGNTASGASSGEDEETACPIKTK"
     gene            complement(2068..>2616)
                     /gene="map"
                     /locus_tag="PK442_07460"
     CDS             complement(2068..>2616)
                     /gene="map"
                     /locus_tag="PK442_07460"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOO87523.1"
                     /translation="INSEIVHGIPSRDRFLEEGDIISIDVGTCFSGYYGDAACTYPVG
                     RVSPVREKLLSVTKKSLEEALLVAQAGRTLGDIGHAVERCVLESGYGIVRDYSGHGIG
                     KYLHEPPQIPNYGQPGRGITLKSGMTLAIEPMVMTGSEDVVTGSDQWVVKTADGSDAA
                     HFEKSVLVKDGDPEILTPWAWA"
BASE COUNT          489 a          790 c          659 g          678 t
ORIGIN      
        1 atggccatgc aggacaagct gcctcgcctg ttttctgctg ttggcgaatc cgaggcggta
       61 caccacgttg tccagtcgtc gctcaagcag gctgaggaag ttgtggcccg tctgccccga
      121 ctgcttgttg gcaatctcga agaagcgctt gaactgcaac tccgtcatgc cgtaaaaacg
      181 gcgaagcttc tgtttttcac gaagacgaat accgtactca ctgactttgg tacggggacg
      241 tcctcttttt ccttttactg tagcgccttc ttttttggtg atggcgcact tagcggtgta
      301 gcagcggtct cccttgagga agagctttgt tccctccgcc ttgcacaaac ggcacgcagg
      361 tccgatatat ctgctcatta gaaataattc cctccttgac caaaaccagg gctagactct
      421 gcgccgcttc ggcgggcggc agccgttgtg gggcactggc gtcgaatcct ttatcatgtt
      481 gacctgcagg cctgccgcct gcagagaacg gattgccgat tcccggccgg gtcccgggcc
      541 cttgacgacg acgtcgatct cctggacgcc gtgttcctgc acagccttgg cgacctgcga
      601 cgcggcgatc tgcgccgcga agggggtgga ctttctggtg cccttgaatc cgacgttccc
      661 gcctgaagcc caggcgagga cgtttccgcc cttgtcgctc acgcacacga tagtgttgtt
      721 gaatgtggag tagatgtgcg cgacgccgta gctaatattc tttctttcct tcttcttacc
      781 tctgcggact gttcgcttgg ccacttgttc cagcctcctt gttctctatc cgaaggctac
      841 ttcgtagcca tctttttgcc cgccaccgtg cggcgagggc ctttgcgggt gcgcgcgttc
      901 gtcttggtct tctgtccgcg gaccggaagg ccgagcttgt ggcgctgtcc tctgtagcag
      961 ccgatgtcca taagacgttt gatgttcatg gacacctctc tgcgaaggtc gccttccacc
     1021 ttgtagtgat cctcaatctc cctgcggagt ttctgggact cctcctcggt aagatccttc
     1081 acgcgcgtgt ccggacttac tccggtgacg gcaagaatct tcttcgaggt gggaaggccg
     1141 attccgaaaa tatatgtcaa cgctatctcc acacgcttct cgcggggaat atcaactccg
     1201 gcgatacggg ccattcgcgc ttacctcctt gcaccctgtc tctgcttatg ccggggattc
     1261 ctgctgcaga taatccgcac gacaccctta cgccggatta cccggcaaaa ctcgcacatc
     1321 ggctttaccg atggttttac tttcattgat tccacgctcc tctgaaagaa ctgaaacaga
     1381 ggcccttcag ggcttctgaa aatatctttt ctccaagcgg tttattatac aggagatcgg
     1441 acaatatgaa aatactctta tttatatcta tagatgattc tgcccctgga caggtcgtac
     1501 ggagagattt ccaccagaac cctgtccccc gggaggatgc ggataaaatg aaggcgcatc
     1561 tttcccgaaa cgtgggcgag tatcttgtgt ccgttatcga gttccacctt gaacatcgcg
     1621 tttggaagcg gctcgattac ttttcctcgt acttctatta cttcgtcttt attggacatg
     1681 cggtctcctc atcctccccg gaggaggcgc cggacgctgt gtttcccgga tcgcagccga
     1741 ctgacccccc gatactgttc ttatttgcaa gccccgtaag cagagcctga aaccttcctg
     1801 cgtcaaagtt acctttcgac aggatactgt gcgcaatttc gtcaaggacc catcgcgtcc
     1861 gctggaggtg gatcatgttt tttgccttgg gtttcccgac agtgaacttc tttccgtccg
     1921 ccaccaaaac cctcgctccc tgcgttcttc gagatggccc gcagtcctct gcaacgccga
     1981 ctacgacgta ccatcttccg gcgtcctttc ctttccgaga tctgaccact tggccaattg
     2041 tgcacatccc gccatcggaa agttcattca ggcccatgcc cacggcgtca gtatctccgg
     2101 atccccgtct ttgacgagaa ccgacttttc aaaatgagca gcatccgagc cgtcggccgt
     2161 tttgacaacc cactggtcgc ttccggtgac aacatcttcg gaacccgtca tcaccattgg
     2221 ttcgatggcc agcgtcatcc cggacttgag ggtgatccct cttccgggct gaccgtagtt
     2281 cggtatctgg ggcggttcgt gcagatactt ccctataccg tgtcccgaat agtcccggac
     2341 aataccataa ccgctctcca acacgcagcg ttccaccgcg tgtccgatat cgccgagcgt
     2401 tctgccggcc tgcgcaacga gaagcgcttc ttcaagactt ttcttcgtta cggagagaag
     2461 tttctccctc accgggctca ctcttcccac ggggtaggta catgcggcgt cgccgtaata
     2521 tccggaaaaa caggttccga catcaatact gatgatatcg ccttcctcca ggaagcggtc
     2581 gcggctgggt attccgtgga cgatctcgga gttgat
//
LOCUS       DAOSWW010000340         2614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30249_length_2614_cov_2.778038, whole genome shotgun sequence.
ACCESSION   DAOSWW010000340 DAOSWW010000000
VERSION     DAOSWW010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30249_length_2614_cov_2.778038"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK442_07465"
     CDS             <1..318
                     /locus_tag="PK442_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87524.1"
                     /translation="VESVGPSSSDAVSKEEGAAEERSPGREAQEAFGGEDDGVENEEG
                     SGSEWSGDVPDLENMTVMELRRLARTVGLVTMTRKQIRFAGKAELLRELMAFWKNRGG
                     ESE"
     gene            315..1784
                     /locus_tag="PK442_07470"
     CDS             315..1784
                     /locus_tag="PK442_07470"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HOO87525.1"
                     /translation="MTQLQDRDLLSVQEVRNLVAKAKEAQKRLASMSQGKIDELVAAV
                     AEAACRHAERLGRMAHEETGFGKPEDKTLKNLFAAKSVYESIKDLRTVGIVREDTKNR
                     MFEVAVPVGVIAGLIPSTNPTSTAIYKAMVALKAGNGIVFSPHPSAKGCILETIRVLS
                     EAVCAAGGPEGIFGCMTIVTLEGTEELMHSKDVALILATGGSGMVRAAYSSGTPAIGV
                     GPGNTPAFIERSADIPQAVRRIISSKTFDYSTICASEQSVVAESCIEQVVRAEFEKQG
                     AYFLPPEDAAKVARAIFTPSGGMNPRTVGKSPKFIAEKAGVAIPEGTKLLLADGMGVG
                     KEYPFSHEKLCPALAFYSADGWEKCCELCIELLAFEGAGHTLAIHSSNDEVIREFGLK
                     KPVSRVVVNAGSSLGAVGATTNLVPSMTLGCGAVGHNATSDNVGPLHLLNIRRVAWGV
                     REIEDLRPAKQAGPAMLNGDQLDRLVERIVEELAGKLKL"
     gene            1852..2139
                     /locus_tag="PK442_07475"
     CDS             1852..2139
                     /locus_tag="PK442_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOO87526.1"
                     /translation="MNNGMQALGMIETKGLVGAIEAADAMVKAANVTLIGKVMVGGGL
                     VTVMVRGDVGAVKAATDAGAAAAGKVGELISVHVIPRPHGEVEFILPKLEG"
     gene            2188..>2614
                     /locus_tag="PK442_07480"
     CDS             2188..>2614
                     /locus_tag="PK442_07480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HOO87527.1"
                     /translation="MNPETRPGQCSGETPGMGAEALELLVGRVVAEVRKRLSFEVEAS
                     GRHVHLNREHVDALFGKGHTLTDSRGLSQPGQFLCSERVTLAGPKGAIENVAILGPER
                     KQSQVELSITDATMLGVKAPVRDSGDLEGSAPLRLRTPKG"
BASE COUNT          489 a          754 c          960 g          411 t
ORIGIN      
        1 gtggagtccg tcgggccgtc ttcctccgat gccgtttcca aggaggaggg cgctgcggag
       61 gagcgttccc ccggtcgcga agcgcaggaa gcgttcggcg gcgaggacga cggcgtcgaa
      121 aatgaggagg gttccggttc cgaatggagc ggcgacgtcc ccgacctcga gaacatgacg
      181 gtgatggagc ttcgccgcct ggcccgcacc gtcgggctgg tgacgatgac acgaaagcag
      241 atacgcttcg ccggaaaggc ggagctgctg cgcgaactca tggcgttttg gaagaaccgg
      301 ggaggtgaga gcgagtgacg cagctacagg accgggatct gctctccgta caggaggtcc
      361 gcaacctcgt cgcgaaagcg aaggaggcgc agaagcgcct cgcctccatg agtcagggca
      421 agatcgacga actcgtggcg gcggttgccg aagcggcgtg ccggcacgcg gagcggctgg
      481 ggaggatggc gcacgaggag accggcttcg gcaagcccga ggacaagacc ctcaagaacc
      541 tcttcgccgc gaagagcgtg tacgaatcga tcaaggacct ccggacggtc ggcatcgtcc
      601 gcgaggatac gaagaacagg atgttcgagg tcgccgtccc cgtcggcgtc atcgcggggc
      661 tgattccctc gacgaacccg acctcgacgg cgatttacaa ggcgatggtc gcgctgaagg
      721 cggggaacgg catcgtcttc tccccgcacc cgagcgcgaa ggggtgcatc ctggagacga
      781 tacgcgtgct ctccgaggcg gtctgcgcgg ctggaggccc ggaagggatc ttcggctgca
      841 tgacgatcgt caccctcgaa ggaacggaag agctgatgca ctcgaaggac gtggcgctga
      901 ttctggccac gggcggatcg gggatggtcc gggcggcgta cagctccgga actccggcaa
      961 tcggcgtcgg cccgggaaat accccggcct tcatcgagcg gtcggcggac attccgcagg
     1021 ccgtccgcag aatcatttcg agcaagacgt tcgactacag caccatctgc gcctcggagc
     1081 agtccgtggt ggcggagtcg tgcatcgaac aggttgtgag agccgaattc gagaagcagg
     1141 gcgcatactt cctgccgccc gaagacgcgg cgaaggtggc ccgcgccatc tttacgccgt
     1201 cggggggaat gaacccccgc acggtgggga agagcccgaa gttcattgcc gagaaggcag
     1261 gggtcgccat cccggagggg acgaagctgc tgctcgcgga cggtatgggc gtggggaagg
     1321 agtacccctt ctcgcacgag aagctctgcc cggcgctcgc cttttactcg gcggacgggt
     1381 gggagaagtg ctgcgagctg tgcatcgagc tgctcgcgtt cgagggcgcg gggcacacgc
     1441 tggcgattca ctcgtcgaac gacgaggtca tccgggagtt cgggctgaag aagcccgtgt
     1501 cccgcgtggt ggtgaacgcg ggctcctctc tgggagccgt gggcgcgacg acgaacctgg
     1561 tcccctccat gacgctcgga tgcggcgcgg tagggcacaa cgcgacgtcg gacaacgtgg
     1621 ggccgctcca tctgctgaac atccggcggg tggcgtgggg cgtgcgcgag atcgaggatc
     1681 tgcgccccgc aaaacaggcg ggtcccgcga tgctgaacgg cgatcagctc gaccgtttgg
     1741 tcgagcgtat cgtagaagag ttggctggga agcttaagct gtaaaaaaga gcgcgttaaa
     1801 gcgcgcgaca ttctttgatt cacacatact tccgagggag gttgcagaga gatgaataac
     1861 ggaatgcagg cgcttggaat gatcgaaacg aagggacttg tcggcgcgat cgaggctgcc
     1921 gacgcaatgg tgaaggccgc gaacgtgacg cttatcggca aggtgatggt gggcggcggt
     1981 ctcgtgaccg tgatggtgcg cggcgacgtc ggcgcggtga aggccgcgac ggacgcgggc
     2041 gctgcggcgg ccgggaaggt cggggagctt atctccgtcc acgtgattcc gcgtccgcac
     2101 ggcgaagtcg agttcattct tccgaagctg gaaggctaat tccggagccc cgtgctccgg
     2161 agtcggcccc ggaagggagc ggcagccgtg aaccctgaaa cgcgtcccgg acagtgctcc
     2221 ggggaaacgc ccggaatggg cgctgaagcg cttgaactgc tcgtcgggcg ggtggtcgcc
     2281 gaggtccgga agcgcctctc cttcgaggtg gaggcctccg gacggcatgt ccatctgaac
     2341 cgcgagcatg tcgacgcgct cttcgggaag gggcataccc tcaccgactc gcgcgggctt
     2401 tcgcagccgg ggcagttcct ctgctccgag cgcgtgacgc tggccgggcc gaagggcgcg
     2461 attgaaaacg tagcgatact cggccccgag cggaagcaga gccaggtgga gctttccatt
     2521 acggatgcga cgatgctcgg ggtgaaagcc cccgtccgcg actccgggga tctggagggg
     2581 agcgcgccgc ttcggctgcg gactccgaag gggg
//
LOCUS       DAOSWW010000341         2612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30283_length_2612_cov_3.879155, whole genome shotgun sequence.
ACCESSION   DAOSWW010000341 DAOSWW010000000
VERSION     DAOSWW010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30283_length_2612_cov_3.879155"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1511)
                     /locus_tag="PK442_07485"
     CDS             complement(108..1511)
                     /locus_tag="PK442_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO87528.1"
                     /translation="MEKNLNNSHAMGTDSITRLMWRFSLPAIVGMLANALYNIVDRLF
                     VGQTVGAAGIGAISVVFPFMILVISFDLLVGVGAGSLISISLGEKRRARAERAMGNAI
                     MLLVGGGILLVLLGVTFTESILELSGASATLMPLCLEYMNIAVWGVFFSTLAFGLNFF
                     IRAEGNPRYAMFTLIIGAGANVLLDALFILVFDMGVGGAALATLFSQMISALWAALYY
                     IRRMGTLRFRMKNLAPDWVITRRILAIGSAPALTEVSFTYVLALFNRLLSFYGGDLAI
                     SAMGIFFSLDSLFFLPVLGLAGGVQPIISYNYGAKDYDRVIRSVLAAIKVGALFFTVS
                     FTLVMLFPAPLIRIFNSDDPELLALGVRGMRLAYSGVIFASVSLIASHTFQAIGKAKT
                     GLFLTLSRHFIFILAPLYFLPPILGMDGIWLSLPLSDLGGALTGTWFLRREFRIMRNA
                     ERLQQISAPGECSVSPD"
     gene            1606..2163
                     /locus_tag="PK442_07490"
     CDS             1606..2163
                     /locus_tag="PK442_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87529.1"
                     /translation="MTEAFLFPLRLHLLCAPSHRRGEYLLERKFAQAFAASNGIPLDF
                     DALMATLREWCASQGVVRNGQTASFSGKSADKKYSGTATRFRDELSILIHVDGEGRKR
                     FRILGVWNDFSWLVLYQEPLLGEWRSWPGAAKDPEGMEKDRTDERSAREGFEWVCRRK
                     IISRAQLLRGGEVTTEYYSPSYRKR"
     gene            complement(2192..>2612)
                     /locus_tag="PK442_07495"
     CDS             complement(2192..>2612)
                     /locus_tag="PK442_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOO87530.1"
                     /translation="LVIESYANPFRTYPLAVDYERFKTLFDDGVACYILNTGEFMGKK
                     VKPADTLGILEAIVEGKAEFVSFGPFSDMETMALDRFPIDFTDEQYRRELFSRMRDRL
                     AFVTSRETEKGGMDRLPPEAVAALRKALDEMGYPVQE"
BASE COUNT          595 a          753 c          801 g          463 t
ORIGIN      
        1 ctcctccgta gagacctcgc aaaccccttc tggaaacgga tgtacggatg cgcgcactcc
       61 cttcactcct cactcatttg tgaaataaag ctccaccggt attatactca atccggggag
      121 acgctgcact cgccgggagc ggaaatctgc tgcagacgtt ctgcattcct catgatacgg
      181 aactcccgcc gcaggaacca cgttccggtg agcgcgcctc caaggtcgga gagcggcagc
      241 gacagccaga tgccgtccat tccgagaatc ggcggcagaa agtacagcgg ggcgagaatg
      301 aagataaagt gccttgagag cgtcagaaag agccccgtct tcgccttgcc gatcgcctga
      361 aaggtatgcg acgctatgag gctcaccgag gcaaagatga cgccgctgta ggcaagtcgc
      421 attccgcgca cgccaagggc cagaagttcg gggtcgtccg agttgaaaat gcggatgagc
      481 ggcgcgggaa agagcatgac gagcgtgaag ctcaccgtga agaagagggc cccgaccttg
      541 atcgcagcca ggacggagcg gatgacccgg tcgtagtcct ttgcgccgta gttgtagctg
      601 atgatcggct ggacgccgcc cgcaaggccg aggaccggca gaaaaaagag actgtcaagg
      661 ctgaagaaga tgcccatcgc cgagattgcc agatcgcctc cgtaaaagct caggagccgg
      721 ttgaagaggg cgagcacgta cgtaaaactg acctcggtga gcgcaggcgc cgaaccgatt
      781 gcaagaatcc tgcgggtgat cacccagtcg ggggcaaggt tcttcatcct gaaccggagc
      841 gtccccattc tgcggatgta gtacagcgca gcccacaggg cggagatcat ctgtgaaaac
      901 agcgtcgcga gagcggcccc tccgacgccc atgtcgaata ccagtatgaa gagcgcgtcg
      961 agaaggacgt tcgctcccgc gccgatgatg agcgtgaaca tggcgtagcg cgggtttccc
     1021 tcggccctga tgaagaagtt gagcccgaag gcaagcgtgg agaagaagac gccccatacg
     1081 gcaatgttca tgtactccag acagaggggc atcaaggtag cgctggcgcc cgaaagttcg
     1141 agaatagact ctgtgaacgt gacgcccaaa agaaccagaa ggattccgcc gcctacaagg
     1201 agcatgatcg cgttccccat cgcccgttcc gccctggcac gccgcttctc gccgagggag
     1261 atggagatga gcgagcctgc gccgacgccc accagcagat cgaaggagat cacaagtatc
     1321 ataaagggga agaccacgct gatcgcgccg atgcctgcgg cgcccaccgt ctgtccgacg
     1381 aagagccggt cgacgatgtt gtagagcgcg ttggcgagca taccgacgat ggcgggaagc
     1441 gaaaatcgcc acatgagacg agtgatcgaa tcagtcccca tcgcgtgcga gttgttcaga
     1501 tttttttcca taagagcctt cctccgcgta caaatttgtt tgaggtatga ttatagcaga
     1561 gagcgccccg caataaaggg gcgagaatat ctccggggag gattcatgac ggaagcgttt
     1621 cttttcccgc tgcgtctcca tctgctctgc gccccctcgc accggcgggg cgaatatctg
     1681 ctcgaacgga aattcgcgca ggcattcgcc gcgtcgaacg gcattcctct ggacttcgac
     1741 gcgctgatgg caacgctgcg cgaatggtgc gcctcgcagg gggtcgtccg caacggacag
     1801 acggcgtcct tctccgggaa gagcgcggac aaaaagtact cgggaacggc cacgcgcttc
     1861 cgcgacgagc tgagtattct catccacgtc gacggagagg ggcgaaagcg cttcagaatc
     1921 cttggcgtct ggaatgattt ttcatggctg gtgctctacc aggagcctct tctgggagag
     1981 tggcgttcat ggcccggtgc ggcgaaagat cccgagggga tggagaagga caggacggac
     2041 gaacggagcg ctcgcgaagg gttcgaatgg gtttgcaggc ggaagatcat ctcccgtgcg
     2101 cagcttcttc gcggcgggga ggtgacaacg gagtactatt ctccctcgta cagaaaacga
     2161 tgagccgggg acatgccccg gctcaaaaac gctactcctg aacgggatac cccatctcgt
     2221 cgagcgcctt ccgcagcgcg gccaccgctt ccggaggcaa gcggtccata ccgcccttct
     2281 ccgtctcgcg ggacgtgacg aacgcgagcc tgtcccgcat ccgggaaaaa agttctcttc
     2341 tgtactgctc gtcggtgaaa tcgatcggga agcgatccag cgccatcgtc tccatatcgc
     2401 tgaaggggcc gaacgacacg aactccgcct tcccctcgac gatagcctca aggatgccca
     2461 gtgtgtcggc gggctttact ttcttcccca tgaactcgcc ggtattcagg atataacacg
     2521 caaccccgtc gtcgaagagc gtcttgaagc gctcgtagtc gacggcaagc ggatacgttc
     2581 ggaacggatt ggcgtaggat tcgatgacga gc
//
LOCUS       DAOSWW010000342         2606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30397_length_2606_cov_3.581341, whole genome shotgun sequence.
ACCESSION   DAOSWW010000342 DAOSWW010000000
VERSION     DAOSWW010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30397_length_2606_cov_3.581341"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK442_07500"
     CDS             <1..761
                     /locus_tag="PK442_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOO87531.1"
                     /translation="DNSKGDLGFSVTSFVGAAGKGEDPFKAPAKNAVVMSNIYTQFTY
                     FIMRKDFAEQHGIKTLGDIVTKKLPVRFATLKPGTASEVTIRALFKKGYNVGWDDFRS
                     WGGKVEFASYDDGANLLADNHLDIFAFSVGRVASIVMQIENQVDVVLLPVEQSALDAL
                     TAAYGTETFMIEPGIYKSVTEPTPTVGDYTCVVIRKDLPEDLVYELSKALWANRESLA
                     LAVKDVEELDPKMALPTAVEAHPGAVKFWKELKK"
     gene            855..>2606
                     /locus_tag="PK442_07505"
     CDS             855..>2606
                     /locus_tag="PK442_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HOO87532.1"
                     /translation="MRQLRGNTQKLFYVYVVAMGLFHLYTAIFGNYEAYLQRSIHLTW
                     VLPMCFVLYPISSKSPKEYVPLYDWILAFISTLPGIYNMINYTHIIERIAQVDPLTTT
                     QLVLGTLLLVILLEATRRVVGVPLTIIAAFFAGYMYFGHHMPGIMKGLSFTFEEVIEH
                     IYLTGEGIFSVPLGVSAAFVMIFLIFGGFLEKSGVGEYFMHLAEAFTGTQAGGPAKIA
                     VVSSALFGSISGSAVANVYGTGTFTIPLMKRIGYPAHFAGAVEAVASAGGQIMPPIMG
                     AGAFIMASFLGRPYSEIMIAATLPAILYYGAVIFMVHLGALKNGLQGLEPDELPSKKE
                     VLKKLYFILPIIGLVYMLLDGYTPMWAATIGIGLTWAISMLNPGKRMTPIDAIDAIYT
                     GAKNIPMVCIACATAGIVVGSVGLTGFGFKFVGFIFAFAKDTPFVALLLVMFVSLILG
                     MGLPTTGAYILAAALGVPALAKLGFPTLSAHLFVFYFAIVSNITPPVALAAYAASSIA
                     GSSPNKTGFTAMRLGILAFIVPFAFCYDTGLLLNAGFFNNITALAGGVAATFALGYAM
                     IGFTRRPLALWQRAFFCI"
BASE COUNT          515 a          879 c          660 g          552 t
ORIGIN      
        1 ctgacaatag caagggcgat ctcgggtttt cggtaacatc gttcgtcggc gcggcgggca
       61 agggagagga tcccttcaag gcccccgcga aaaacgcggt cgtcatgtcg aacatataca
      121 cccagttcac ctacttcatc atgcgcaaag atttcgcgga acagcacgga atcaagacgc
      181 tcggcgacat cgtcacgaag aagcttcccg tacgcttcgc cacgctgaag ccgggaaccg
      241 cctccgaagt taccatccgc gccctcttca aaaaggggta caacgtaggc tgggacgatt
      301 tccgcagctg gggcggcaag gtcgagttcg cctcctatga cgacggcgcg aaccttctgg
      361 ccgacaacca cctcgacatc ttcgccttct ccgtcggccg cgtcgcatcc atcgtcatgc
      421 agatcgaaaa ccaggtcgac gtcgttctcc tcccggtcga acagagcgcg ctcgacgctc
      481 ttacggcagc atacggcacc gagacattca tgatcgaacc gggcatctac aagagcgtca
      541 ccgagcccac cccgaccgtc ggcgactata cctgcgtcgt catccgcaaa gacctccccg
      601 aggatctggt ctacgaactc tccaaggcgc tgtgggcaaa tcgtgaatca cttgccctgg
      661 ctgtgaagga cgtcgaggaa ctcgacccca aaatggcgct cccaacggcc gtcgaggcgc
      721 atccgggcgc ggtgaagttc tggaaggaac tcaagaagta gctccttcat aatctccgca
      781 ctgagcgcgg gcggtggcga atcaccgccc gcttttaatt ttgaaaagaa cgacctagga
      841 agtgagtgta cacaatgaga caactacgag gaaacaccca aaaactcttt tacgtgtacg
      901 tagtcgccat gggactcttt cacctgtata ccgccatctt cggcaactac gaagcgtacc
      961 ttcagcgaag catccacctg acatgggttc tcccgatgtg cttcgtcctc tatccgatat
     1021 cctccaaatc tccgaaggag tacgtcccgc tctacgactg gattcttgca tttatctcga
     1081 cgctgcccgg catctacaac atgatcaact atacccacat catcgagcgc atcgcccagg
     1141 tggatcccct gaccacaaca cagcttgtcc tgggcacact gctgcttgtc atcctgctcg
     1201 aagccacccg gcgcgtcgtc ggcgttccgc tgacaatcat cgcggccttt tttgcgggct
     1261 acatgtactt cggccaccac atgcccggca taatgaaggg cctctctttc acattcgaag
     1321 aggtgatcga gcacatctac cttaccggcg aaggaatttt ttcggtaccg ctcggtgtct
     1381 ccgcggcgtt cgtcatgata ttcctcatct tcggcggctt ccttgaaaaa agcggcgtcg
     1441 gcgagtactt catgcacctt gccgaagcct tcacaggcac gcaggcaggc ggcccggcga
     1501 agatcgcggt cgtcagctcg gccctcttcg gctccatctc gggctccgca gtcgccaacg
     1561 tttacggaac gggtactttc accatccccc tgatgaagcg catcggctac cccgcgcact
     1621 tcgccggagc cgtagaggcc gtcgccagcg ccggagggca gatcatgcct ccgataatgg
     1681 gagccggcgc cttcatcatg gcatccttcc tcggccgccc ctacagcgag atcatgatcg
     1741 ccgcaacact ccccgcaata ctctactacg gcgccgtcat cttcatggtg catcttggcg
     1801 cactcaagaa cggcctgcag ggcctggaac cggatgaact cccctccaaa aaagaagtgc
     1861 tgaagaagct ctacttcatc cttcccatca tcggcctcgt ctacatgctg ctcgacgggt
     1921 acacgccgat gtgggcggca actatcggca tcgggctcac atgggcgatc tccatgctca
     1981 accccggtaa gcgtatgacc cccatcgacg ccatcgacgc gatctacacc ggcgccaaga
     2041 acattcccat ggtgtgcatc gcgtgcgcca ctgcgggtat cgtcgtcggc tcggtcggtc
     2101 tcaccggctt cggcttcaag ttcgtcgggt tcattttcgc ctttgccaag gacaccccct
     2161 tcgtcgcgtt gctgcttgtc atgttcgtct cgctgatcct cggcatgggg cttccgacga
     2221 caggcgccta catcctcgcc gccgccctcg gcgttcccgc cctggcgaag ctcggtttcc
     2281 cgacgctctc cgcacacctc ttcgtcttct acttcgccat cgtctcaaac atcacccctc
     2341 cggttgcgct tgccgcatac gccgcaagct ccatcgccgg gtcttccccc aataaaacgg
     2401 gctttacggc aatgcgtctc ggtattctcg ccttcatcgt ccccttcgct ttttgttacg
     2461 acacgggact gctgttgaac gccgggttct tcaacaacat caccgcgctc gcgggaggcg
     2521 tcgccgctac cttcgccctg gggtacgcca tgatcggctt cacgaggcga ccgctcgccc
     2581 tctggcagcg cgcgttcttc tgcatt
//
LOCUS       DAOSWW010000343         2596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30611_length_2596_cov_3.917355, whole genome shotgun sequence.
ACCESSION   DAOSWW010000343 DAOSWW010000000
VERSION     DAOSWW010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30611_length_2596_cov_3.917355"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PK442_07510"
     CDS             complement(<1..1137)
                     /locus_tag="PK442_07510"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HOO87533.1"
                     /translation="MQIQIWSDMNWRLILTLVLLSGVLAYLGDVLGMRIGKRRISLFG
                     LRPRDTSRVITAFTGILISIAILITMTVISDNVRTALFSMKFIQGQLQTLTRDLQKSR
                     DEAELMAINLVGSETKLQEQQKKLAGVQAELESVAPLLDEARASLVRTQTEKEQVEQE
                     KAALSRSVEELKEEAEALRKGLIQVRSGRISVFAMEILPQAPVEPMSSRSDVERIFQE
                     LRKQAEFVVAARTGQPSADVLLSVDLEKEAERIAECSGQPSRKFVRAIAEGNAVFGED
                     IRVVYEVYDSVLVYRKGELLTRRTLKPYEGINAETELHLILRMVNRKAVADDIKPDPV
                     TGNVGTLDGAQFFEAVDRLTRADGPVLVEVTAAEDIHTEGPVRVS"
     gene            complement(1201..1617)
                     /locus_tag="PK442_07515"
     CDS             complement(1201..1617)
                     /locus_tag="PK442_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOO87534.1"
                     /translation="MWWEEGLRFTCLGCGRCCRGEPGAIFFTPEEEEKIATFLSLPVS
                     EFRRRYVTSRWRAPSLKEKNNGDCVFYDAAADRCFIYRVRPLQCRLFPFWPALLESEE
                     AWNQAAALCPGMNQGDFHTAREIRGLLGLNPFPDLL"
     gene            complement(1618..1983)
                     /locus_tag="PK442_07520"
     CDS             complement(1618..1983)
                     /locus_tag="PK442_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87535.1"
                     /translation="MQIDPRFASFVPSNRGIAESSVALKGTKEREKLKEACQQFESIL
                     LAEIWKKMNANARSIGGKSDSDRPFGPMEDLAVEMSAEQLAKEGGAGMWRMLYDQLVV
                     HLDSAEELARRRRPEEASE"
     gene            complement(1984..>2596)
                     /locus_tag="PK442_07525"
     CDS             complement(1984..>2596)
                     /locus_tag="PK442_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOO87536.1"
                     /translation="TVGRIPGGAIVERNVQTTFSAGRQINLLLRNPDFTTSERMAAAI
                     NSAYGGIASPVDAGRVVVNVPPQYASSPAAFLAHIEGISLTPDTAARVAVNERTGTVV
                     MGGNVRIGAVAVAHGNLTVRVAENPEVVQPQPFGGGTTAVQPRTDIGINESPGQFVAL
                     DSTSTVQELVDALNSVGASPRDVIAILQAIKEAGALHGELVVM"
BASE COUNT          464 a          778 c          787 g          567 t
ORIGIN      
        1 ggagacgcgt accggacctt ccgtatgaat gtcctcggcc gcggttacct cgacaagcac
       61 cggtccgtcc gcccgtgtga gacggtccac ggcttcaaag aactgcgccc cgtcgagcgt
      121 tcccacattt ccggtgacgg ggtcgggttt gatatcgtcg gcgactgctt ttctgttcac
      181 catccggagg atgaggtgca gctcggtctc cgcgttgatt ccttcgtagg gtttgagcgt
      241 ccgccgggtc aaaagctctc cctttctata gacgaggacg ctgtcgtaca cttcgtagac
      301 gacgcggata tcctccccga aaaccgcatt tccctcagct atcgcgcgga cgaacttcct
      361 cgacggctga ccggaacact cggcgatacg ctccgcctct ttttccagat cgacggaaag
      421 gagcacgtcg gcggacggct ggccggttct ggcggcaacg acgaactccg cctgttttcg
      481 cagctcctgg aagatgcgtt cgacgtcgct gcggctgctc atcggctcga cgggcgcctg
      541 agggagtatt tccatggcga agacggagat gcggccgctg cgtacctgga tgagcccctt
      601 cctcagggct tccgcttcct ccttgagctc ttcgacggag cgtgagagcg cggccttctc
      661 ctgctccacc tgttcttttt ccgtttgagt gcggacaagg cttgcgcggg cctcgtcgag
      721 caggggcgcg acgctctcca gctccgcctg gactccggcg agcttcttct gctgctcttg
      781 gagtttggtc tcgctgccga cgaggttgat cgccatgagc tccgcttcgt cgcggctttt
      841 ttgaagatct cgtgtgagcg tctgaagctg tccctgaatg aatttcatac tgaagagggc
      901 ggtgcggacg ttgtccgata tgaccgtcat ggtgataagg atggcgatgg agatgaggat
      961 gccggtgaag gcggtgatca ccctgctggt gtcgcgtggg cgaagcccga agagagagat
     1021 gcggcgtttg cctatacgca ttccaaggac atccccgagg tacgcgagga cgccgctgag
     1081 gaggaccagc gtgagaatga gcctccagtt catgtccgac cagatttgga tctgcatgcg
     1141 cactcccctc gatcttcttc cgctgaaacg tccggacgcg tgttcgcgtc aacgaaacta
     1201 ttatagcagg tcgggaaagg gattgagccc gagcagcccg cgaatctccc ttgccgtatg
     1261 aaaatctccc tggttcatgc ccggacacaa cgcggctgct tgattccacg cctcttcgga
     1321 ttcaagcagg gcgggccaaa agggaaagag acggcactga agcgggcgga cccggtagat
     1381 gaagcatctg tcggcagccg cgtcgtagaa gacgcagtcc ccgttgtttt tctccttcag
     1441 gctgggcgct ctccatctcg aggtgacata ccggcggcga aactcactca ccggaagaga
     1501 gaggaaagtt gctatctttt cctcctcttc cggcgtgaaa aagatcgctc ccggttctcc
     1561 gcggcagcag cggccgcatc cgagacaggt gaaacggagt ccttcctccc accacactta
     1621 ttcggacgcc tcttccggac ggcgtcttct tgcaagctcc tcggctgaat ccagatggac
     1681 gacaagctgg tcgtagagca tccgccacat tcctgcgccg ccctccttcg ccagctgttc
     1741 cgcggacatt tccacggcaa gatcctccat tggtccgaag ggacggtcgg agtcgctttt
     1801 gccgccgatg ctgcgcgcgt tggcgttcat ttttttccag atctccgcca gaaggatgga
     1861 ttcgaactgc tggcaggctt ctttcagttt ttcccgttct ttcgttcctt tcaacgctac
     1921 ggatgattcc gcaattcccc tgttggaggg aacaaaagaa gcaaaacgcg gatcgatctg
     1981 catttacatc accaccagtt ccccgtgaag cgccccggcc tctttgatgg cctggagaat
     2041 cgcgatgacg tcccgcggcg atgcgcctac ggaattcagc gcgtccacca gctcctgcac
     2101 agtgcttgtg gagtcgaggg cgacgaattg accgggagat tcgttgattc cgatgtcggt
     2161 acgaggctgc acggcggtcg ttcccccgcc gaacggctgc ggctgcacga cctcagggtt
     2221 ttccgccacg cggaccgtga ggtttccgtg agcgacggcg acggcgccga tacgcacgtt
     2281 tcctcccata acgaccgtgc cggtgcgttc attgaccgcg acccgtgccg cggtgtcggg
     2341 ggtgagcgaa attccttcga tatgggcgag aaaggctgcc ggcgacgatg cgtactgggg
     2401 tgggacattc acgacgaccc tgcctgcgtc cacaggggag gcgatgcctc cgtaggcgga
     2461 gttgatcgcc gcggccatgc gctcagaggt cgtaaagtcg gggttgcgga gcaacaggtt
     2521 gatctgccgt ccggcggaga atgtggtctg gacgttccgt tcaacaatgg cgcctccggg
     2581 aattctgccg acggtg
//
LOCUS       DAOSWW010000344         2588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30749_length_2588_cov_4.266088, whole genome shotgun sequence.
ACCESSION   DAOSWW010000344 DAOSWW010000000
VERSION     DAOSWW010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30749_length_2588_cov_4.266088"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="PK442_07530"
     CDS             complement(<1..174)
                     /locus_tag="PK442_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HOO87537.1"
                     /translation="MFSESGGTGTRTGDRLPIIAIVGPTAVGKTAVSLLLAERLGAEI
                     ISVPVDQPYGARDY"
     gene            complement(180..1988)
                     /gene="mutL"
                     /locus_tag="PK442_07535"
     CDS             complement(180..1988)
                     /gene="mutL"
                     /locus_tag="PK442_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOO87538.1"
                     /translation="MYSRIAAGEVVERPVSVVKETVENALDAGATEISVALHEGGKLR
                     IVIEDNGEGMSFDDLPLAVARHATSKIRTVEELETVRSLGFRGEALASIAAVSRLEIR
                     SRKAGEEQGALLSIEGGRTVRHVPTPCRPGTRISVEDLFYTLPARRKFLKTAAAEARR
                     VSALVRDLAVAYPAVAFLEQHDGKQVFSSPGDGDRESLLRAMWGDPRQAPRTSSGRAL
                     DAGFSGAELRMCEAAATNLSLECWWMPFPGKTRSSISTFVNGRTVTDSVIRGAVGALC
                     RSLVGNWIFFFALSPELLDANIHPAKAEVRFRYPGEVFDVVQQAVLTLSGKPQTIAPF
                     SAGVSSDGFDARRNAPLLGHQGNASGPSRSWQRQDASDGELFNRVAEAPFSRETATAP
                     PQSEESMGIFPTEQEAQRGEERDSVRLLGRMESGYVVFEDGDALAVMDPHAAHERIGY
                     ERARRLSKDSVTRQRCAVPLPLPPSFSMSVREHMEALEDIGFSFDEREGLLLLTVYPA
                     LQGGIGEDPVRLLRSVLLEWTEDRKSPLEDILWRKLATLACGLSVKLGDRLTASECLA
                     LWRGLMQCEQPWSCPHGRPTVLLLPRNKLESFFGRE"
     gene            complement(1993..>2588)
                     /locus_tag="PK442_07540"
     CDS             complement(1993..>2588)
                     /locus_tag="PK442_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOO87539.1"
                     /translation="TANILNNLTERSLVILDEVGRGTSTYDGMSIAWAVLEHLAEGCG
                     CSPKVLFATHYHELTCLDERYPGIENLSMAVKEDSDGIFFLHQVVKGSADRSYGIEVA
                     RLAGLPRPVLKRAFELLQRFEKDEKCAAPPQHAAKRKEIPGIRQMTLFESARSGIVEE
                     IAALTPDTLTPFRALELLYSLVEKSREAIRSENLSSS"
BASE COUNT          486 a          895 c          763 g          444 t
ORIGIN      
        1 atagtcgcgg gcgccgtagg gctgatccac cggcaccgag atgatctccg cgccgaggcg
       61 ttccgcaagc agaaggctga cggcggtttt tcccaccgcg gtcggcccta caatggcgat
      121 gatcggcaga cggtcgcccg tcctcgttcc ggtaccgccg gattctgaaa acatgcagtc
      181 tactcccttc cgaagaacga ttccagttta tttcgcggca gaagcagcac ggtcggccgt
      241 ccatgagggc acgaccacgg ctgttcgcac tgcatcaggc ccctccataa ggcgaggcac
      301 tcggacgctg tcagccggtc ccccagcttg acggagagac cgcaggcaag cgtcgccagc
      361 tttctccaga gaatatcctc cagcggactc ttccggtcct ccgtccactc cagaagcacc
      421 gagcgcagaa gacggacagg gtcctcccct atacctccct gcagcgccgg gtagaccgta
      481 aggaggagaa gcccctcgcg ctcgtcgaag gagaagccga tatcctcaag agcctccatg
      541 tgctcgcgga cggacatcga gaaggacggc ggaaggggga gcggaaccgc gcagcgctgc
      601 cttgtcaccg aatccttcga gagcctgcgc gcccgctcat atccgatccg ctcgtgcgcc
      661 gcgtgcgggt ccatcaccgc aagagcgtcc ccgtcttcga agacgacgta ccccgactcc
      721 atacgcccca acagccgcac ggagtcgcgc tcctcgcccc tctgcgcttc ctgttcggtc
      781 gggaagattc ccatgctttc ttccgattga ggaggagcgg ttgcggtctc tcgggaaaac
      841 ggcgcttccg cgaccctgtt gaagagttcg ccgtccgaag catcctgacg ctgccatgag
      901 cggctcggcc ccgaggcgtt cccctgatgc ccaaggagcg gagcgttccg acgcgcatcg
      961 aatccgtccg aagacactcc cgcagagaac ggagcgatgg tctgcggttt tccggagagc
     1021 gtgagcacgg cctgctgcac cacgtcgaag acctctcccg gataccggaa gcgcacctcg
     1081 gcctttgcag ggtggatatt cgcgtccagc agctcgggcg aaagagcgaa aaagaaaatc
     1141 cagttgccca ccagcgatct gcacagagcg cccaccgcgc cccgaatgac ggaatccgtc
     1201 accgtccgtc cgttcacgaa tgtcgaaatg gagcttctcg tcttcccggg gaagggcatc
     1261 caccagcact ccagcgaaag attcgtcgcc gccgcctcgc acatacggag ttccgcgccc
     1321 gagaacccgg cgtcgagcgc tctgccggag gaagtacgcg gcgcctgacg gggatccccc
     1381 cacatggcgc gaagcagcga ctctctgtcg ccgtcccccg gagaggagaa gacctgcttg
     1441 ccgtcgtgct gttccaaaaa agcgacggcg gggtacgcca cggcgagatc gcgcaccagc
     1501 gccgaaactc gccgcgcctc cgccgccgca gttttcagga attttctgcg cgccgggagc
     1561 gtgtaaaaaa gatcctcgac ggagatgcgc gtccccggac ggcagggcgt cggcacatgg
     1621 cgaacagtgc gccctccctc gatcgagagc aacgcgccct gctcctcccc ggctttccgc
     1681 gaacgaatct ccaggcggct cacggcggcg atgctcgcca gcgcctcccc ccgaaaaccg
     1741 agcgagcgga cggtctccag ctcctcgacg gtgcggattt tactcgtcgc gtgcctggct
     1801 acggccagag gcagatcgtc gaacgacatt ccctcgccgt tgtcctcgat gacgatacgg
     1861 agctttccgc cctcgtggag agctacggaa atctccgtcg ctcccgcgtc aagcgcgttc
     1921 tccaccgtct ccttcaccac ggaaacgggg cgttcgacca cctctcccgc cgcgatgcgg
     1981 gagtacacgt cctcaggaag aagagagatt ttcagagcga atcgcctccc tgcttttctc
     2041 gaccagcgaa tagagtaatt ccagcgcgcg gaagggtgtc agcgtatccg gcgtcagcgc
     2101 cgcgatctcc tcgacgatgc cggagcgcgc ggactcgaac agcgtcatct gccggattcc
     2161 ggggatctcc ttccgtttgg cggcgtgctg cggcggcgcg gcgcatttct catccttctc
     2221 gaagcgctgc aacagctcga acgccctctt gagcacaggg cgaggcaatc ccgcaagacg
     2281 cgcgacttcg ataccgtacg accggtccgc gcttcctttc accacctgat gcagaaagaa
     2341 gatgccgtcc gaatcctcct tcacggccat gctcaggttc tcgatccccg gatagcgctc
     2401 gtcgaggcag gtcaactcgt ggtagtgcgt cgcgaagagc accttcggcg agcagccgca
     2461 cccctccgcc agatgctcaa ggacggccca ggcgatgctc atgccgtcgt aggtggacgt
     2521 cccgcgcccc acctcgtcga ggatgaccag actcctctcc gtcaggttgt tcaggatgtt
     2581 cgccgtct
//
LOCUS       DAOSWW010000345         2587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30773_length_2587_cov_2.729463, whole genome shotgun sequence.
ACCESSION   DAOSWW010000345 DAOSWW010000000
VERSION     DAOSWW010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30773_length_2587_cov_2.729463"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PK442_07545"
     CDS             complement(<1..544)
                     /locus_tag="PK442_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO87540.1"
                     /translation="MKEVVVGVVGGGFASHFHSRCFARVSGFSVRLKAIADVEIEKAK
                     AVAEAYRYEEYHDDYSALLRDPEIDVVDICTPPFLHPRITMEAMEAGKHVICEKPLAG
                     YFGSPGDIEPIGDTVPKSRMYQSVLTEIETMKAALEGSDRLFMYAENYVYSPNILKAA
                     EIVRAKKSKILFMKGEESLKG"
     gene            complement(881..2161)
                     /locus_tag="PK442_07550"
     CDS             complement(881..2161)
                     /locus_tag="PK442_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO87541.1"
                     /translation="MTGVLFGSLVFFFLLNVPIAVAIGLASLSAILWSGAIPPVVLVQ
                     KMFTATDSFPLMAVPFFILAGSLMEFGGISRRLVDFANSIVGRFSGGLAFVAIIASMF
                     FGAISGAAVACVAAIGTILIPAMVRRGYDTPYATAVQATAGTLGVMIPPSIPMIIYGV
                     LTGVSIGALFMGGILPGILVGGSLMLVAWMIARKRGYKGDEKASGAKIWATFKDAILA
                     LLMPVIILGGIYGGVFTPTEAAVVAVAYGFIIGFFVYRELSLSKLKDILVNTSVGTAT
                     IMFIIATSSVFSWILTAQRVPEAVAEAILSISTNPIVILTLINLLLLFIGTFMETVAS
                     IIILVPVLLPVVTQIGVDPLHFGIIIVVNLAIGMVTPPLGVCLFVGCGISGITLESIS
                     KAVWPFILSMIVAVLLLTYIPWISIILPRLAGIY"
     gene            complement(2158..>2587)
                     /locus_tag="PK442_07555"
     CDS             complement(2158..>2587)
                     /locus_tag="PK442_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO87542.1"
                     /translation="VMVVVVFLQVIFRFIIRSSLPWSEELSRYVMVWIVFLGASIGIK
                     RKSHIGVEAVVAMLPDSMRRWTAILVNALCCVFFVFMVHYGRMILRVVSSQLSPAMEI
                     SMAIPYSAVFVGGTLMFVHCFFQMVELMVEALSGRAVKSA"
BASE COUNT          676 a          741 c          676 g          494 t
ORIGIN      
        1 atcccttcag gctctcctcg cccttcatga agagaatttt gcttttcttc gcccgcacta
       61 tctcggcggc cttcagaatg ttcggcgagt agacgtagtt ttcggcgtac ataaagagcc
      121 tgtcgcttcc ttcaagcgcc gccttcattg tttctatttc cgtcaacacg gactggtaca
      181 ttctggactt gggaacagta tcgcctatcg gttcgatgtc tcccggagag ccgaagtacc
      241 cggcgagcgg tttttcacaa atgacatgct tccccgcctc catcgcctcc atcgtgattc
      301 gcgggtgcag aaaaggggga gtgcatatat cgacgacgtc gatctccgga tcgcgcaaaa
      361 gcgcgctgta atcgtcatgg tactcctcgt aacgataggc ttccgcaacg gccttcgcct
      421 tttcgatctc gacgtccgcg atggctttca aacgcacgga aaaccccgaa acccttgcga
      481 aacatctgct gtgaaaatgc gaggcgaatc cgcctccgac aacgcccaca acaacttctt
      541 tcattctatg tcacctcgcg attgtgaaga aaaagtaaga aggaaaagag agccctccga
      601 ccgaggggca gggattctcc gctcaaaaaa gcgcaccata tcccgctcta cgggaataac
      661 atccgctaaa aggacttcga ggttacaata caaaacgcgg cataccctgt caagactatt
      721 tccatattca aagatcagca taacgagagc gatttgttga tttatctaca cgcttgcgct
      781 gacttggcgt atccgttcga gtatcgcgat ctctttagtc caattgaaaa agcgccccga
      841 aacgatacgt ttcgaggcgc ttttgcgtgc agcaagtttt ctaataaatc cccgccagac
      901 gaggaagaat gatgcttatc caagggatat acgtcagcag tagcaccgcg acaatcatgc
      961 tcaggatgaa cggccacacc gctttggaga ttgattccag cgtgatcccg ctgattccgc
     1021 aaccgacgaa gaggcagacg ccgagcggag gggtgaccat tcctatggca aggttcacca
     1081 caataatgat gccgaagtgg agcggatcga cgcctatctg cgtcactacc ggaagaagaa
     1141 cggggaccag gatgatgatg gaggcaaccg tctccatgaa ggtgcctatg aagagcaaaa
     1201 gaaggtttat gagcgtgagg atgacgatcg ggttcgtcga gatcgagagg atagcctcgg
     1261 ccacagcctc aggtactctc tgcgccgtca gaatccagct gaagaccgac gaggtcgcga
     1321 tgatgaacat gatcgtcgcc gtgccgaccg aagtgttcac caggatatcc ttgagcttcg
     1381 agaggctcag ttcccggtag acgaaaaatc cgatgatgaa gccgtaggcc accgcgacga
     1441 ccgccgcctc cgtcggggta aagactcctc catagatgcc gccgagaatg atgaccggca
     1501 tcaggagcgc gagaatcgca tccttgaagg tcgcccatat ttttgcgccg cttgcctttt
     1561 catcgccctt gtaccccctc ttgcgcgcaa tcatccacgc aacgagcatc agggatcccc
     1621 cgacgaggat accggggagg atgccgccca tgaacaacgc gccgatggag acgcccgtga
     1681 gcacgccgta aatgatcatc ggaatgctcg gcgggatcat gacgccgagc gttcccgcag
     1741 tcgcctgcac cgcagtggcg tagggcgtat cgtatccgcg ccggaccatt gccggaatga
     1801 ggatcgttcc gatggcggct acgcaggcta ctgcggctcc ggagatcgcg ccgaaaaaca
     1861 tgcttgcgat aatggcgacg aaagcgagtc cgcctgaaaa cctgccgacg attgagtttg
     1921 cgaaatcgac caggcggcgg gagattcctc cgaactccat caacgacccg gcaagaatga
     1981 agaagggaac cgccatgagg gggaaggaat cggtcgcggt gaacatcttc tgcacaagca
     2041 ccacgggcgg gatcgcgccg gaccagagga tcgccgagag cgaggccaat ccaatggcca
     2101 ccgcaatcgg aacgttcaac agaaagaaaa agacaagact tccgaagaga actccggtca
     2161 tgcgctcttc accgcccttc ccgaaagagc ttcgaccatc agttccacca tttgaaagaa
     2221 acagtgaacg aacatcaacg ttccgccgac aaaaaccgcg ctatagggaa tcgccatcga
     2281 tatctccatt gcaggagaga gctgcgaact cacaaccctg agaatcatcc tgccgtaatg
     2341 gaccatgaaa acgaagaaaa cacagcacag cgcgttgacg agaatagccg tccagcggcg
     2401 catcgaatcc ggcaacatcg cgaccacggc ctccacgccg atatgggatt tccgcttgat
     2461 gccaatgctc gctcccagga aaacgatcca taccataacg tatcgggaca gctcttccga
     2521 ccacggaagc gaagagcgaa tgatgaaacg gaaaataacc tgaaggaaca ccaccacgac
     2581 catcacc
//
LOCUS       DAOSWW010000346         2584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30828_length_2584_cov_3.046659, whole genome shotgun sequence.
ACCESSION   DAOSWW010000346 DAOSWW010000000
VERSION     DAOSWW010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30828_length_2584_cov_3.046659"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..718
                     /locus_tag="PK442_07560"
                     /pseudo
     CDS             437..718
                     /locus_tag="PK442_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            828..>2584
                     /locus_tag="PK442_07565"
     CDS             828..>2584
                     /locus_tag="PK442_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO87543.1"
                     /translation="MAFGGWTPPASDVAADPLAIACKSLSDAGILLVAAAGNEGQNIA
                     RAGGGYKGNTIYPAAFAFPEMVTVAAITQNGALAKFSNFGSSVDIAAPGYEVKSTALG
                     GAYASRNGTSVASAHVAGAAALLAAASPELTAGQIKTRLLEYAAGHQTLSGKVASGGS
                     LNIGAAIKNERGSLKVLIEGPASARWTLNGNGSYPSGQVLTDLPAGKHTVSFSDANLW
                     KKPANIAVSITSDSQAEATGKYRQQNASIWVTIEGPEEARWSLNKKGEYTSDQTLGKA
                     AGKRTVSFSEVPGWDAPAPQSVTVPRNAPLAVSGVYTRQTGSVTVTFEGPAEARWSLD
                     GQGSYSSGETVENVPVGNRTVSFAKIEDWDTPQDVVVLVSKGASVAAEGKYIHQVGSI
                     KVTLEGPKEASWNVNGRGLFASGETASNIPVGEHTIAFSAVQDWDHPAAVPVTVVRDE
                     TVEISRAYSKHLGSVSVTIKGPSDARWSLDGIDGMFESGAVRADLPVGEYAVVFKDVE
                     NWYAPEKMNVTVVKDEVAQREASYAEHRGSVIVEIDGPAEARWSLDGEGSYASGESAK
                     DVVVGKHTVSFSDIADWDKP"
BASE COUNT          591 a          701 c          788 g          504 t
ORIGIN      
        1 actgggcgag gtttttattc gatctatatc acagcacgaa tggatggtga tgggaatgaa
       61 aaaaacgaaa cttgcgttga tcctgctttt cctgttcctg tgcgccggct ccgcctttgg
      121 ccagccgaaa gcgccgagcg acgaatatgt ccggggagat gttcttgttg ttcttgaagc
      181 gcccgggggc gcttccgagg tagagagcca agcgaaccgg atcgccgaag cgacctcttc
      241 gcgggtgatt cgcacctata gcgcgattgc cgccgcaaca gggaaaaata tcgtgcagct
      301 caaggcggac ggtaaatcag cggaagaact gctcaccgca gttcgccaaa ttcccggagt
      361 gctgggcgcg acgccgaact acatcttcaa aatcctccga acgccgaatg atccagagtt
      421 tccgtcctcc tggggtatgg agtctataaa cgcatccgga gcatgggaaa tatccaccgg
      481 cagcgaggag gtctatgtag ctcttcttga tacgggaata cagtacgacc atgaggatct
      541 ggcagccaac atgggacgcg acaaagacgg caacttcgga agaaacctgg tgagcgcggc
      601 gaacgcgaca aatccgatgg acgataacgg ccacggaacg caccttgcgg gtattatcgg
      661 cgcagcggga gacaacgggc tgggcgcagc cgggatcaac tggactgtca aactgctggc
      721 ggtcaaggtc cttgacggga acggcgcggc tagtgtcggt cacatcatcg ccggactcga
      781 ctacgtgctg gagcagaaaa gcaaaggact caacccgggt tgcgaatatg gccttcggag
      841 gatggactcc tccggcatcc gacgtcgctg ccgatcctct ggcaatagcc tgcaaatccc
      901 tctccgacgc aggtatcctc ctggttgccg cggcagggaa cgaggggcag aacatagcaa
      961 gagcgggcgg cggatacaaa gggaatacga tctatcccgc agcctttgcg ttcccagaga
     1021 tggtgacagt agctgcgatt acacagaacg gagctcttgc taaattttct aactttggtt
     1081 cttctgtgga tattgccgcc ccaggctacg aagtaaagag cacggccttg ggtggagcat
     1141 acgcttcgcg caacggtacg tcggttgctt ccgcgcatgt cgcgggcgct gcggcccttc
     1201 ttgcggcggc gtccccggag ctgacggcgg ggcagatcaa aacacgtctt cttgagtatg
     1261 ccgcgggcca tcagacgctc tccggtaagg tagcgtccgg cggttccctg aatatcggcg
     1321 ccgcgataaa gaatgagaga ggttctttga aagtcctcat cgaagggcct gcatcggctc
     1381 gctggacctt gaacggaaat gggagctatc caagcggcca ggttctgaca gacctccccg
     1441 ccggaaagca tacagtctcc ttctcggacg cgaacctctg gaaaaagcct gcgaatattg
     1501 cagtatcgat aacgtccgat tctcaagccg aagcgacagg aaaataccgc cagcagaacg
     1561 cgtccatctg ggtcaccatc gaaggacccg aggaggctcg ctggagcctg aacaagaagg
     1621 gcgagtacac aagcgaccag actctgggga aggccgccgg aaagcgaact gtttccttct
     1681 ccgaggttcc cggatgggac gctcctgcgc ctcagtccgt aaccgttccc aggaatgcgc
     1741 cgctcgccgt gtccggagtc tacacgcgcc agaccggttc tgtcacggta acattcgagg
     1801 gaccggccga agctcgctgg agtcttgacg gccagggatc ctattcgagc ggggaaactg
     1861 tagaaaatgt tcctgtgggc aaccgtacgg tgagctttgc caagatagag gactgggata
     1921 cgcctcaaga cgtagttgtt cttgtttcta agggcgcttc cgtcgcagcg gaagggaagt
     1981 atatccatca ggtcggatca ataaaagtga ctcttgaggg tcctaaggag gccagttgga
     2041 atgtgaacgg cagagggctt tttgcgagcg gcgagacagc ctcgaacatt cctgtcggtg
     2101 agcacacgat cgctttttcc gccgttcagg actgggatca tcccgctgcc gtcccggtga
     2161 cggtggtccg tgacgaaaca gtggaaatct caagggcgta ttccaagcac ctcggctcgg
     2221 tctccgtcac gatcaaggga ccctccgatg ctcgctggag cctcgatggg atcgacggaa
     2281 tgtttgagag cggcgcagtt cgcgcggatc tcccggtcgg cgagtatgcc gttgtcttca
     2341 aggacgttga aaactggtac gctccggaga agatgaacgt taccgttgtc aaagacgagg
     2401 ttgctcaacg agaagcctcc tacgccgaac acagaggatc cgtcatcgtt gaaatcgacg
     2461 gtcccgccga agctcgttgg agcttggacg gcgaggggag ctacgcaagt ggagagagcg
     2521 caaaagacgt cgttgtcggg aagcacacag tctccttctc cgatattgcc gactgggaca
     2581 agcc
//
LOCUS       DAOSWW010000347         2580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30905_length_2580_cov_3.807129, whole genome shotgun sequence.
ACCESSION   DAOSWW010000347 DAOSWW010000000
VERSION     DAOSWW010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30905_length_2580_cov_3.807129"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PK442_07570"
     CDS             <1..1671
                     /locus_tag="PK442_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOO87544.1"
                     /translation="AAWATAALAFAGLPVVSFGAVGAMIMALHITGRYIESRLRDKAS
                     KEIRALLSIQAREARVLDGEGKEIMIPIEAVKEGMTLLVKPGERLPSDGELLDGTTSV
                     DESMITGESLPVVKNAGDGVTGGSLNLTGAVRIRATRIGEDSFLAQMVSLIQEAQGAK
                     VPIQAFADRVTNYFVPGVATLAVISGVFWYFQAGRLAPFLDGAARFLPWVTSLRDPLS
                     LSIFAFITTIVIACPCALGLATPMALITGTGAASKKGLIIRNAEAIQTARDVTVAVLD
                     KTGTITEGKPTVVETDLSPEALAAVASLEAFSNHPLAKAIAAASDERLPVTDLEEITG
                     EGVRGKVAGTTWFVGRPGDASKYDAHLALGQTVVEVRRRDAVTGFVAVEDPLREDAIE
                     GIRRLSEMGISCVMATGDNEKTARAVAARVGITEVRAGVRPDGKLDLVRNLQAKGGKV
                     LMTGDGMNDAAALKGADIGVAIGSGTDLAIDSADIVIVKGGISRLADAVAISRKTFTV
                     IRQNLFWAFAYNVIAIPLAMAALLHPVIAEGAMAFSSISVILNSMRVK"
     gene            1813..>2580
                     /locus_tag="PK442_07575"
     CDS             1813..>2580
                     /locus_tag="PK442_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO87545.1"
                     /translation="MKLRSILKENAVDIVLFLAAALLAGGYFLYGTGGSATWYVAGLL
                     LLFILMFFKSIFSCSGPAAVVSEWIGRLSRNESVDLRKSVDVPQGSKLSSVANALNAF
                     VQVMRTHFLRIMQGLHKFTFNFYRLERQLGEFFSAFSAMSAGVKEGISSGQNVSHATE
                     SQYASSEEISATAQGLAQLAAELNNAVASAGDRADAGNRRLREMERTFASVVEETKTL
                     SEEARILSGKVDVIQGVVHTITGIAEQTNLLALNASIE"
BASE COUNT          454 a          815 c          859 g          452 t
ORIGIN      
        1 gcggcgtggg cgacagcggc cctcgcgttc gcggggcttc cggtggtgtc gttcggcgct
       61 gtgggcgcga tgatcatggc gctccacatc accggacggt acatcgaatc gcgcctgcgg
      121 gacaaggcgt cgaaggagat ccgcgcgctg ctctccatcc aggcgcgcga ggcgcgcgtg
      181 ctcgacggcg aggggaagga gatcatgatc cccatcgagg ccgtcaagga ggggatgacg
      241 ctgctggtga aaccgggcga acgcctcccg agcgacgggg agctgctcga cgggacgact
      301 tcggtggacg aatccatgat cacaggagaa tcgctccccg tggtgaagaa cgcgggcgac
      361 ggcgtgacgg gcggctcgct aaacctcacc ggggccgtcc gcatccgcgc cacccgcatc
      421 ggcgaggaca gcttcctcgc gcagatggtc tcgctgatcc aggaggccca gggggcgaag
      481 gtgccgatac aggccttcgc cgaccgcgtg accaactact tcgtgccggg cgtggcgacg
      541 ctcgcagtga tcagcggcgt cttctggtac ttccaggccg gacggcttgc ccccttcctc
      601 gacggcgcgg cgcgttttct cccgtgggtg acctcgctgc gcgatccgct ctcgctctcc
      661 atcttcgcct tcatcaccac catcgtcatc gcgtgcccgt gcgcgcttgg gctggcaacg
      721 ccaatggccc tgatcaccgg cacaggcgcg gcctcgaaga aggggctcat catccgcaac
      781 gccgaggcca tccagacggc gcgggatgtg acagtagccg tgctggacaa gacgggcacc
      841 atcaccgaag gaaaaccgac cgtcgtggag acggaccttt ctcccgaggc gctcgcggcc
      901 gtggcatcgc ttgaagcctt ctcgaaccac ccgctcgcca aggcgatcgc cgccgcgtcc
      961 gacgaacgcc tccccgtgac cgacctggag gagatcaccg gcgagggcgt gcgaggaaag
     1021 gtcgcgggta cgacctggtt cgtcgggcgt cccggggacg cctcgaagta cgacgcccac
     1081 ctcgcgctcg ggcaaacggt ggtcgaggtc cggcgccgcg acgccgtaac aggcttcgtc
     1141 gcggtggagg acccgctgcg tgaggacgcc atagaaggaa tccgccgcct ctcggagatg
     1201 ggcatctcct gcgttatggc gacgggcgac aacgagaaga cggcccgtgc cgtggccgca
     1261 cgcgtgggca tcaccgaggt gcgggcgggc gtccgccccg acggaaagct cgacctggta
     1321 cggaacctcc aggcgaaggg cggcaaggtg ctgatgacgg gcgacggcat gaacgacgcc
     1381 gccgcgctca agggcgccga catcggcgtg gcgatcggct ccgggacgga tctcgccatc
     1441 gacagcgcgg acatcgtcat cgtcaagggc ggcatctcgc gtcttgccga cgctgtggcc
     1501 atctcgcgca agaccttcac ggtgatccgg cagaatctct tctgggcctt cgcctacaat
     1561 gtgatcgcca tcccgctcgc catggccgcg ctgctccacc ccgtcatcgc cgagggcgcg
     1621 atggcgttca gctccatctc ggtgatattg aactcgatgc gggtgaagta gcgtgtgtcc
     1681 gcgaacggag agaggcggtc cgaacagatg aagccgccgt tccggtgtgc gggtttgatg
     1741 aaaacgcttt gttttcgata agaggtatgg tatcgtttca tggaaacaat acgatgaagg
     1801 tggggtcatt caatgaagct ccgaagtata ctcaaagaaa acgcggttga tatcgtcctc
     1861 tttcttgcgg cggcgctcct tgcgggcggt tatttcctgt acgggacggg cggaagcgcg
     1921 acctggtacg tggctggact cctcctgctt ttcattctta tgttcttcaa gagcattttc
     1981 agctgctccg gccctgccgc tgtcgtctcc gagtggatcg ggcggctttc gcggaacgag
     2041 tcggtcgatc tgcgcaagag cgtcgacgtg ccccagggct ccaaactgag ctcggtggcg
     2101 aacgccctca atgcgttcgt tcaggtcatg cggacgcact ttctgcgcat catgcagggg
     2161 ctgcacaagt tcacattcaa cttctaccgt ctggagcggc agctcggcga gttcttttcg
     2221 gccttctccg cgatgtccgc aggcgtgaag gaggggatct cctccgggca gaacgtctcg
     2281 catgcgacgg agtcgcagta cgcctcgtcc gaggagattt cggccaccgc gcaggggctg
     2341 gcgcagctgg cggccgaact gaacaacgcc gtcgcctccg ctggcgacag ggcggacgcc
     2401 gggaacagac gcctccgcga gatggagcgc accttcgcct ccgtcgtcga ggaaacgaag
     2461 acgctttcag aggaagcgcg tatcctctcg ggcaaggtgg acgtcattca aggcgtggtg
     2521 catacgatta ccgggatagc ggaacaaacc aacctgcttg cgctcaacgc ctcgatcgag
//
LOCUS       DAOSWW010000348         2580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30910_length_2580_cov_2.709307, whole genome shotgun sequence.
ACCESSION   DAOSWW010000348 DAOSWW010000000
VERSION     DAOSWW010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30910_length_2580_cov_2.709307"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PK442_07580"
     CDS             complement(<1..597)
                     /locus_tag="PK442_07580"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOO87546.1"
                     /translation="MMTPSAIAPCRRYPKHASGSGGAKRSLSAVFRGPPVRQGGSARG
                     DRYAPLFACPPIWRRLAGALALLLICLFPLASGAEEKILSFVSAATVFKDSSLEVRED
                     ISVNVEGRRIQRGIYRDFPTKYSDSTGRSVILGFSVKSALLGGREVPYTVRSIADGVR
                     VYLGDPDGRAPIGEQTYTLVYVVAGALGFFDDHDELYWN"
     gene            complement(753..950)
                     /locus_tag="PK442_07585"
     CDS             complement(753..950)
                     /locus_tag="PK442_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HOO87547.1"
                     /translation="MQGTERADIKPGMKVLVVQKQDQRSGALTEGVVQSLLTKSPHHP
                     HGIKVRLENGIVGRVKEIIEE"
     gene            1261..2025
                     /locus_tag="PK442_07590"
     CDS             1261..2025
                     /locus_tag="PK442_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87548.1"
                     /translation="MKRTDRIFALLLFFSLLLSGLPPLEASENADPSEWTFSTTSYVW
                     FSGLNGTLGVKGHKARVNLSFSDVLSNMDVSGMLTFQGHKGKQYFFFDGMYLDMSASE
                     QVSPQLFTDVGATTTRLQAAWGVRVKEWESSSLHVFGGLRYWNLKNELTFRTASAPLR
                     HFSESESWIDPLVGIRFATQLSPNLTFLAILDAGGFGIGSDFTWGGMLDLSWKLSERT
                     SLELGYRYLYVDYEKDGFVMDAYNDGIFIGFTWKLR"
     gene            2077..>2580
                     /locus_tag="PK442_07595"
     CDS             2077..>2580
                     /locus_tag="PK442_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO87549.1"
                     /translation="MRKTGIMSIMLLSSLLCPATLCFASDYAFTLDESGIPALAGERS
                     AIVVISDLHLGMDDRYAETQKNRAPLVDFLQKLRRAPNIKELVIAGDLVDEWFIPAGV
                     DTYAGKTQKEFAMAVAANNEEVFDAFNSIIQDGKIKVTYVPGNHDLLVDSESIQTILP
                     GISETRDV"
BASE COUNT          569 a          793 c          693 g          525 t
ORIGIN      
        1 gttccagtag agttcgtcgt ggtcgtcgaa aaagccaagc gccccggcaa cgacatagac
       61 gagcgtatac gtctgttcgc cgataggcgc tcttccgtca ggatcgccaa ggtagacccg
      121 tacgccgtcc gcaatggaac gcaccgtgta tggcacttcc cgtccgccga gcagggcgct
      181 cttcacggaa aagccgagga taacggagcg tccggtcgaa tcgctgtact tcgtcgggaa
      241 atcccggtag atgccgcgtt gaatccgccg cccctcgaca ttcaccgaaa tatcctcgcg
      301 cacttcgagc gaagaatcct taaacacagt cgccgcagaa acgaacgaga ggattttctc
      361 ctccgccccc gaggcaagcg gaaacaggca gatcaagagc agcgcgagcg ctccggcgag
      421 acgtctccaa atcggaggac aagcaaaaag cggagcatac ctgtcgccgc gggcgcttcc
      481 tccttgccgg acaggcggcc cccgaaaaac tgcggagaga ctgcgcttcg ctcctccgct
      541 cccgctcgca tgtttcgggt atctcctgca gggggctatc gcggacggcg tcatcatgcc
      601 tcccctccct catgatagaa tgatgaacct cttgtaaagt attctatcat gttcgtgcac
      661 gtcatccatg cgcgtacaca caatgcccgg cgtcagaagc gagcagcgcc agccgtgcgt
      721 ctcgttgcgc cgtacccttg gttacggcgc tgttactcct ctattatctc cttcacacgc
      781 cccacgatgc cgttttcgag gcggaccttg atgccgtgcg gatggtgcgg gctcttcgtc
      841 agcagcgact gcaccactcc ttcggtaagc gcgcccgagc gctggtcctg cttctgcacc
      901 accaacacct tcattccggg cttgatgtcg gcccgttccg ttccttgcat agactcacct
      961 cctgtcaatt tttcgctctc aggggaaaca agccgctgaa tgcgcctgaa aagaacacca
     1021 tcttacaaca gcggaaacaa tcccgaaagg cggcaggcgc ttttagagag cgcctgcgca
     1081 tcacccccgc ggccgcacga aaaaagaata catataaaaa tacattaaac ataaaaaagg
     1141 ctttgaacat atggacaaaa tcacgcacat agcgtatagt ttataggatt gtgcgcacaa
     1201 agagcgcacg agagcgcgca ctgctcagcg aacaaaaaaa gtgcagaggg agtgacgttt
     1261 atgaaacgaa cggaccgtat ttttgctttg cttctcttct tctcccttct tctttccgga
     1321 cttcctccgc ttgaggcctc ggagaacgcc gatccctccg agtggacctt ttccacgacg
     1381 tcgtacgtct ggttctccgg cctcaacgga acgctgggcg tgaaggggca caaggcgcgc
     1441 gtcaacctga gcttctccga cgttctttcg aacatggacg tctcgggaat gctcaccttt
     1501 caagggcaca aggggaaaca gtacttcttt ttcgacggta tgtaccttga catgtcggca
     1561 agcgagcagg tatcccccca attgttcacg gacgtcggcg cgaccacgac ccggcttcag
     1621 gccgcatggg gagtgcgcgt caaggagtgg gagtcgtcct ccctccacgt tttcggcggc
     1681 ctgagatact ggaacctcaa aaacgagctg acgttcagga cggcgtcggc cccgctccgg
     1741 cacttcagcg agagcgaatc ctggatcgac cccctcgtcg gcatccgctt cgcgacacag
     1801 ctttcgccga acctgacctt tctcgccata ctcgacgcgg gcggcttcgg catcgggtcg
     1861 gacttcacct ggggaggaat gctggatctc tcgtggaagc tctcggaacg gacgtcgctc
     1921 gagctgggct accgctatct ctatgtcgat tacgagaagg acggcttcgt gatggacgca
     1981 tacaacgacg gcatcttcat cggcttcacg tggaaacttc ggtagagcgc cgtcgcgctt
     2041 ttaccgatca tatcacgcac gaaagggaat gagactatga gaaaaacggg tatcatgagt
     2101 atcatgctgt tgtcttcgct gctgtgcccc gcaactctat gcttcgcgtc cgactacgcc
     2161 ttcacccttg atgagagcgg cattccggcg ctcgccggcg aacggtccgc tatcgtcgtc
     2221 atcagcgacc tccacctggg aatggacgac cggtacgcgg agactcaaaa gaaccgcgct
     2281 ccgctcgtcg actttcttca gaaacttagg cgcgctccga acatcaagga actcgtcatt
     2341 gcgggcgacc tcgtcgacga gtggttcatc cccgccggcg tagacacgta cgccggaaag
     2401 acccaaaagg agttcgcgat ggccgtcgcc gcgaacaacg aagaggtgtt cgacgccttc
     2461 aacagcatca tacaggacgg gaagatcaag gtgacctatg ttccggggaa tcacgacctg
     2521 ctggtcgact ccgaaagtat ccagaccatc ctgcccggca tatcggaaac tcgcgatgtc
//
LOCUS       DAOSWW010000349         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30925_length_2579_cov_2.759509, whole genome shotgun sequence.
ACCESSION   DAOSWW010000349 DAOSWW010000000
VERSION     DAOSWW010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30925_length_2579_cov_2.759509"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PK442_07600"
     CDS             complement(<1..503)
                     /locus_tag="PK442_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO87550.1"
                     /translation="MKHSEHRRKGFSEIGIITTILLFLVPGGKFFVTVPTAMEEFIEG
                     DKMEEMEEVFRKQSIELDDYVQNAALNPPADTAAASTGNLLKGLPLGLFDSLVLTTEE
                     KALMGDIEAQNELGWMYDTGEGMPENVEEAVKWYRKAAEQGNSVAQANLAWMYEKGRG
                     VPLDLQLA"
     gene            complement(597..2288)
                     /locus_tag="PK442_07605"
     CDS             complement(597..2288)
                     /locus_tag="PK442_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87551.1"
                     /translation="MKKCASWYNNATMQARYAQRRTLSFQNPPSCETHSEGASFHSHE
                     YGFRRSGPSIREGGIPVIRKGCFVFFTLLWGLWCVAPLPALAGVHAVDDVEYSTPHGW
                     VQQESPGAKAYHVLMYQGHPVGELFLMKESLFEQKSPATVLQEGIRKNAAGFAGYQAI
                     GMRNISLSGTEAVMHDFLYYPPGSPVQFAGRVVVLVVDKSAYTFFFNTTINTAAMLEG
                     PFSEVVSSVRVIRKPVAPPRKVQEGVFSIELAPGWREANDLKEEGVHCYDYYGDTGED
                     TVLISLLAFGRSKEDESTALSAILQKKNPLEAVLNAMIDMHKRRGKYVPLQTKTRSVA
                     GCDAIVHDFSIQKPDSNFVYRLCIIAVKQQADTPSRVFAPAMYDFTFSSFEMDRFNKG
                     KAAVDALLDSMTLIQPLPALSPPPPFSDLQDERIFRSPTEHFTLTLPEGAKQDAMEQL
                     DPVDVLGESFTYTIEGKKDTIILLSIFYSALDGEFAHEELLDELEAKESGQATWEIQG
                     RNVPVGLYSARGGKALVTALFADDDLLLAVLLPKKEYGAAQGWIKELINGVRFEK"
     gene            complement(2371..>2579)
                     /locus_tag="PK442_07610"
     CDS             complement(2371..>2579)
                     /locus_tag="PK442_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOO87552.1"
                     /translation="VSVTDAEALDAFSRLCRKEGIIPAVESSHALAYALKRLPSMRKD
                     QILLVNLSGRGDKDLETVLPMLGL"
BASE COUNT          506 a          746 c          733 g          594 t
ORIGIN      
        1 gcgagctgca ggtccagcgg gacgcctctg cctttttcgt acatccaggc cagattcgcc
       61 tgggcgaccg agttgccctg ttcagcggcc tttcggtacc acttgaccgc ttcttccacg
      121 ttttccggca tcccttcgcc ggtgtcgtac atccagccga gttcattctg cgcttcaata
      181 tcccccataa gcgccttctc ctccgtggtc aacaccagag agtcaaaaag ccccaaagga
      241 agccccttca agagattgcc cgttgatgcc gcggcagtat ccgccggagg gttcaaagcg
      301 gcattttgga catagtcgtc cagttcaatg gactgcttcc ggaagacctc ctccatctct
      361 tccattttgt ctccttcgat aaattcctcc attgctgtgg gaacagtgac aaagaattta
      421 cctcccggga caagaaacaa aagaatggtc gtgattattc cgatttcaga aaaccctttc
      481 cgccggtgtt cgctgtgttt catgtctcac tccttctttc tgtcgggggt tttagggaag
      541 accttatcgg ttttcccgta atgccgttct tttcaaagag agcgcttccg cagaggctac
      601 ttttcaaagc ggacgccgtt aatcagctct ttgatccacc cctgcgcagc cccgtactct
      661 ttcttcggca gcaggacggc gaggaggagg tcgtcgtctg cgaagagcgc cgtgaccagc
      721 gccttcccgc cgcgcgcgga atacagtccg acggggacgt ttcttccctg gatttcccag
      781 gtcgcctgcc cgctctcctt tgcttccagc tcatcgagga gttcctcgtg cgcaaactcc
      841 ccgtccagcg cactgtagaa gatggacaga agtatgatcg tgtccttctt cccttcaatc
      901 gtgtaggtga acgactctcc gaggacgtcg accgggtcga gctgttccat ggcgtcctgt
      961 tttgctcctt cggggagcgt cagtgtgaag tgttcggtag gagaacggaa gatgcgttcg
     1021 tcctgcaggt cggagaaggg cggaggggga gagagggcgg gaaggggttg aatcagcgtc
     1081 atcgagtcga gcagcgcgtc gaccgccgcc ttccctttgt tgaaacggtc catttcaaac
     1141 gaggaaaagg taaaatcgta cattgcggga gcgaataccc gcgaaggcgt atccgcctgc
     1201 tgtttgaccg cgatgatgca cagacggtag acaaaattcg aatccggctt ctggatggag
     1261 aaatcgtgga cgatcgcgtc gcagcctgcg acagaacggg tcttcgtctg cagagggacg
     1321 tatttccccc ttcgtttgtg catgtcgatc atggcgttca aaacggcttc cagcggattc
     1381 ttcttctgca aaattgccga gagagcggta gactcatcct ccttgctgcg cccgaaggcc
     1441 agtagcgata tcagtacagt gtcctcgccg gtatcgccgt aataatcgta gcagtgaacc
     1501 ccctcttcct tcagatcgtt cgcttcgcgc catccagggg cgagttcgat ggaaaaaacg
     1561 ccttcctgga cttttcgcgg gggcgcgacc ggtttccgga taacccggac ggaggagacg
     1621 acttcgctga acggtccttc gagcatggcg gcggtattga tcgtcgtatt gaagaaaaag
     1681 gtgtaggcgg atttgtccac aacgagcacg acgacccttc ccgcgaactg cacggggctc
     1741 cccggcgggt agtagaggaa gtcgtgcatc acagcctcgg ttccggagag cgagatgttt
     1801 ctcatgccga tcgcctggta ccccgcaaat cctgcggcgt tcttccggat gccttcctgg
     1861 aggactgtcg cggggctttt ctgttcgaag agggactctt tcatcaggaa gagctcgccg
     1921 accggatgcc cctggtacat caggacatgg tatgccttcg ctcccggaga ttcctgttgc
     1981 acccatccgt gcggcgtgga gtattcaacg tcatcaacgg catgcacccc cgcgagcgcc
     2041 ggaagcggcg caacgcacca cagaccccag agtaacgtga agaatacgaa acaccctttg
     2101 cgaatcaccg gtattccccc ttcccgtatc gacggtccgc tgcgcctgaa gccgtactcg
     2161 tgagagtgga acgacgcccc ctccgagtgc gtttcacagg agggaggatt ctgaaagctc
     2221 aaagtgcggc gttgcgcata tcgggcctgc atcgtcgcat tattgtacca cgatgcgcac
     2281 tttttcatca ggttgtcttt ggcgggaaga gcgtagtttt ttgagagaag tgagcgctgc
     2341 cggagggact ctccggcagc gctcgcatgt ctacaggccg agcatcggca gcacggtctc
     2401 cagatctttg tctccgcgtc cggagaggtt gaccagcagt atctgatcct ttcgcatcga
     2461 ggggaggcgt ttcagcgcgt acgccagcgc gtgcgagctt tccacggcgg ggatgatccc
     2521 ttccttcctg cagagacggc tgaatgcgtc cagcgcttcg gcgtccgtga cggagacgt
//
LOCUS       DAOSWW010000350         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_30974_length_2576_cov_3.894090, whole genome shotgun sequence.
ACCESSION   DAOSWW010000350 DAOSWW010000000
VERSION     DAOSWW010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30974_length_2576_cov_3.894090"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..89)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(281..832)
                     /locus_tag="PK442_07615"
     CDS             complement(281..832)
                     /locus_tag="PK442_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOO87553.1"
                     /translation="MTNNTRTDETVQAFLEKLKDRGLRLTAQRETLFRVIAEDLGNPT
                     SVQNIWDRTREVDPSIGIATIYRTLNLLGEMGVVNIIYLSDGEFRLDVPLQKIHLTAF
                     CRHCGALFPLPAEDEKQHTLEKWLEGTGLELLPQSVAVAGLCTECREALKSPDALPAR
                     RGPLGQQGPCLRRRCRRMGRVQE"
     gene            1005..1289
                     /locus_tag="PK442_07620"
     CDS             1005..1289
                     /locus_tag="PK442_07620"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOO87554.1"
                     /translation="MPGRNGTGPMGQGSMTGWGRGPCGRGVAAGGRFFRGRGCFGWGM
                     RMGMGMGRGGVWAEPYTGADEQTLLEQELAMTEARAQELRARLGKGGDAE"
     gene            1286..1648
                     /locus_tag="PK442_07625"
     CDS             1286..1648
                     /locus_tag="PK442_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO87555.1"
                     /translation="MTKAAIAMDSGVISEHFGKTKEFLFITFEGKNEISREILPPPAE
                     EHVPGLFPNWVKKMGADVVVAGGMGTQAKRMFESLGVGVCTVPSMGVEQAVEALLSGS
                     LQGVDMECTHGHDHSCGK"
     gene            1645..2487
                     /locus_tag="PK442_07630"
     CDS             1645..2487
                     /locus_tag="PK442_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87556.1"
                     /translation="MKIAVASGKGGAGKTSIAASLVLAKRGVLAMDLDVEEPNLGILL
                     GCSPGESCPVHQPVAYVEPDLCSGCGRCAQACAYGAIAWFGSGLPVVNESLCHGCGVC
                     PLVCPTGAMREREKVIGEIRSGFVDGAPFLEGRLEVGGVSTVRLIEATIQAGESLHAD
                     DWIMDAPPGASCPVSAVLREADAAILVVEPTPFGLSDVAGVLEVVADMKKPAGIVANK
                     TGMGDLSLDDLCRRFSVEVLARYPFTRSAAEAGARGVCPYRSEETWVWKTESLWRAIE
                     RRFS"
BASE COUNT          457 a          727 c          879 g          513 t
ORIGIN      
        1 cgcctgagag tttcagccgt tctttcgaaa cggccttgcc ccttcggcgt ctcccctcat
       61 tgcggaggag actctcttgc ggcgttcata cggaaagtac attcattgta gcgaatgaac
      121 cgaaagtgtc aagcggcgcg gcggaatagt gcgacggaat tttattataa taaatttgtt
      181 cttgtgaaca aagagtgcgc cgatgaagac tgctccggtt gcacgcgcag aaggggttcg
      241 cgcccgcgct gctttccccg gcgtagtatt cggcggtgac tcactcctga acacgcccca
      301 ttcttcggca gcggcggcgg aggcaggggc cctgttgtcc caaggggcct ctgcgggcgg
      361 ggagcgcgtc ggggcttttc agcgcctccc tgcattccgt gcagagccca gccacggcta
      421 cggactgcgg aagcagttcg agtcctgtcc cctccagcca tttctccaga gtgtgctgtt
      481 tctcatcttc ggcggggagg gggaagagcg cgccgcaatg cctgcagaac gccgtgagat
      541 ggatcttctg cagcgggacg tcgagccgga attcgccgtc gctcaggtag atgatgttca
      601 caacgcccat ctcccccaga aggttgagcg tcctgtagat cgtcgcgatg ccgatcgagg
      661 ggtccacctc gcgcgtcctg tcccagatgt tctgaaccga cgtggggttg cccaaatcct
      721 cggcgatgac ccggaagagc gtttcacgct gtgcagtgag gcgaagcccc cggtccttca
      781 gcttttccag gaaagcctgt actgtctcat ccgtgcgcgt attgttcgtc atggtgtgtt
      841 ccctccctgg ttttgaggaa aattataccc ctccggcggt accggcacgt cccggcgttt
      901 tctccgcctg ccgacccgcg agtatgcatc ttccccttga cataatgcca caggatagcg
      961 tattatgcaa atgataatta ttatcattaa aggaggagat cgagatgccg ggaagaaacg
     1021 gaacgggacc catgggacag gggtcgatga ccggatgggg gcgcggccct tgcgggcgcg
     1081 gcgtcgcagc aggcggacga ttttttagag ggcgcggctg ctttggttgg ggcatgcgta
     1141 tgggaatggg tatgggcaga ggcggcgtgt gggccgagcc gtataccggg gccgacgaac
     1201 agacactgct cgaacaggag cttgcaatga ccgaggcaag ggcgcaggag ctgcgcgcca
     1261 gactgggaaa gggtggggac gcagagtgac gaaagcggcg attgcaatgg acagcggcgt
     1321 tatttccgag cacttcggca agacgaagga gtttcttttc atcaccttcg aggggaagaa
     1381 cgagatctcg cgcgagatcc ttcctcctcc cgccgaagag catgttcccg ggctcttccc
     1441 gaactgggtg aagaagatgg gggccgacgt ggtggtcgcg ggaggcatgg gaacgcaggc
     1501 gaagcggatg ttcgagtcgc tgggcgtcgg tgtctgcacg gttccctcca tgggtgtgga
     1561 gcaggctgtg gaggcgctcc tctccggttc tctgcagggc gtcgatatgg agtgcaccca
     1621 cggacacgac cactcctgcg gaaaatgaag atcgccgtgg cgagcggcaa ggggggcgcg
     1681 ggcaagacca gcatcgcggc ctcccttgtt cttgcaaagc gaggggtgct cgcgatggat
     1741 ctcgacgtgg aggaacccaa tctggggatt cttctggggt gttcgcccgg tgaatcgtgc
     1801 ccggtgcatc aacctgtggc gtatgtggag cccgatctct gctcgggctg cggacgatgc
     1861 gcacaggcgt gcgcgtacgg cgccatcgca tggttcggct ccggccttcc cgtggtgaac
     1921 gaatcgctct gtcacgggtg cggcgtctgt ccgctcgtct gcccgacggg ggcgatgcgt
     1981 gagcgcgaga aggtgatcgg cgagatccgc tccggattcg tcgacggggc gcccttcctc
     2041 gaaggacggc ttgaggtcgg aggcgtgagc acggtacggc ttatcgaggc gacgatccag
     2101 gcgggcgagt cgctgcacgc cgacgactgg atcatggatg cgcctccggg cgcttcctgt
     2161 cccgtgtcgg cggtgctgcg cgaggcggac gccgccatcc ttgtcgtcga gccgacgccg
     2221 ttcggtcttt cggatgtcgc gggtgtgctc gaagtcgtcg ctgacatgaa aaaaccagcg
     2281 ggaattgtcg cgaacaagac cggcatgggc gatctatcgc tggatgacct gtgccgccgc
     2341 ttctccgtcg aggtgctcgc gcgctacccc ttcacccgct ccgccgcgga ggcgggggca
     2401 aggggagtct gcccgtaccg ctccgaggaa acgtgggtat ggaagacaga gtccctctgg
     2461 cgtgccattg aaaggagatt ctcatgagtc cgcgcgagat cgttgtcgtc agcggcaagg
     2521 gcggcacagg gaagacgacc ctcacggcct gttttgcagc gcttgcggag gggccg
//
LOCUS       DAOSWW010000351         2564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_31216_length_2564_cov_2.620566, whole genome shotgun sequence.
ACCESSION   DAOSWW010000351 DAOSWW010000000
VERSION     DAOSWW010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31216_length_2564_cov_2.620566"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK442_07635"
     CDS             <1..200
                     /locus_tag="PK442_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HOO87557.1"
                     /translation="RLLKEHMGGNFLFLNVLLMGGMCGAGVGAPSFDETASAVKALSP
                     AKFEDANMKVFKLGYEAIANS"
     gene            271..720
                     /locus_tag="PK442_07640"
     CDS             271..720
                     /locus_tag="PK442_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87558.1"
                     /translation="MIVVLLVLTILSACIAPALTSSYINVSSPERVRREGERAASWLQ
                     RVFHKALLSGRCFTLSPSSLRSRPNIVVQWTAPVEDEVFRGDGSAFFINNALESPRSF
                     YVPKWNILTPALTIRVSDSEGPRRTAKPLWYIVVSPYGRVSLRDTPP"
     gene            861..1400
                     /gene="lepB"
                     /locus_tag="PK442_07645"
     CDS             861..1400
                     /gene="lepB"
                     /locus_tag="PK442_07645"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOO87559.1"
                     /translation="MAAAKPWWRETIETILWALVLALILRTFVVQAFWIPSGSMIPTL
                     EVGDRVLVAKFWYHFQEPKRGQLFVFKYPVDPKRDFVKRIIGLPGDMLEVRNGAVLIN
                     GQPLQEEYVKNHDSFSLTAGPVFPEVPVKIPANSYFAMGDNRPNSQDSRFWGFVPKQN
                     IRGPVFFRYWPLNRIGMVD"
     gene            1401..2222
                     /locus_tag="PK442_07650"
     CDS             1401..2222
                     /locus_tag="PK442_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOO87560.1"
                     /translation="MGRTVWYPGHMAKGKRKLSELAGKLDIIIEVRDARAPAQTSSPL
                     VGELSSVCPIAVALSKRDLADEKATAKWLSYFAASGQKAWALDLLKPRMDQVRRDLAI
                     FGPAHREVRLAVVGIPNVGKSMFLNALVGKSSAQVGGIPGITRGVSWYKGKGFLAVDS
                     PGILDPKADDAVQRCLAWLGSSKAEVIGGYENVALDLIAVLRRKNLWGMVEAKWNVAA
                     PAETSDEDVLEALGRRLGCLVSGGAVDLSNAARRFVEAFSTGRLGRVTLELPEES"
     gene            2222..>2564
                     /locus_tag="PK442_07655"
     CDS             2222..>2564
                     /locus_tag="PK442_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOO87561.1"
                     /translation="MELKEFGRIAGTDEAGRGPLCGPVVAAAVILTETQRDELLALGL
                     ADSKKLSPRKREALFDRMLSHGVLWRAQAASHERIDRMNILRASLWAMRRSLMKLPPL
                     FDTVFVDGTFTL"
BASE COUNT          473 a          741 c          781 g          569 t
ORIGIN      
        1 atcggctttt gaaagagcac atgggcggga atttcctttt cctgaacgtc ctcctcatgg
       61 gcggaatgtg cggcgcgggc gtaggagctc cttccttcga cgagacggca tcggctgtca
      121 aggcgctttc gccggccaag ttcgaggacg ccaatatgaa agtattcaag ctggggtatg
      181 aggcgatcgc caactcctga acctgtctgt tgtcctgcga ggggaaccct ttcccggggt
      241 tcccctcgca gcgcgttctc ccttgccgag ttgatcgtcg ttcttctggt tctgacaatc
      301 ctctcggcct gtattgctcc tgcgctcaca tcctcgtata tcaacgtctc ctctccggag
      361 cgcgtccgga gagagggcga acgagcggcg tcatggctgc agcgggtttt tcacaaggcg
      421 ttgctctcgg gaagatgttt cactctctcc ccgtcttcgt tgcgctcgcg gccgaatatt
      481 gtcgtgcagt ggactgcgcc cgtcgaggac gaggtctttc gcggagatgg aagcgccttt
      541 ttcatcaaca atgctcttga atccccccgc agtttttacg taccgaaatg gaacatccta
      601 acgcccgccc tgacgatacg ggtgtccgat tccgaagggc cgcgcagaac ggcgaagccg
      661 ctctggtata tcgtcgtctc gccgtacggt cgggtttcgc tgcgcgatac gccgccgtga
      721 tgcggaaagc ggcgtttttg tgtgataatt ttactgtggg attgaaactc ttgctttttc
      781 ggggtataaa agaggccgat actggaaaca tgaaccctct tttccccatc ggttcttcac
      841 tttgaaacgg aggttattgt atggcagcgg cgaaaccctg gtggcgcgag accatcgaaa
      901 caattctctg ggctcttgtt ctggccctca ttctcaggac gtttgttgtg caggcgttct
      961 ggattccaag cggttccatg attcccactc ttgaggtggg agacagggtg ctggtggcca
     1021 agttctggta tcacttccag gagccgaaga gagggcaact cttcgtcttt aagtaccccg
     1081 tggaccccaa gcgggatttc gtcaaacgaa tcatcgggct tccgggcgat atgctcgaag
     1141 tgcgcaacgg cgccgtcctc atcaacggac agcctcttca ggaagagtac gtcaagaatc
     1201 acgacagctt ctcgttgact gcggggccgg tatttccgga ggttccggtc aagattccgg
     1261 cgaactccta cttcgcaatg ggcgacaaca ggccgaattc acaggacagt cgtttctggg
     1321 ggttcgtccc gaagcagaac atccgcggtc ccgtcttttt ccgctactgg cctctgaaca
     1381 ggatcgggat ggtggactag atggggcgta ccgtctggta tcccggccat atggccaagg
     1441 gaaagcgaaa gctttccgaa ctcgccggga aactggacat catcatcgag gtgcgcgacg
     1501 cgcgggctcc ggcgcagaca tcctctccgc tcgtcggaga gctctcttcc gtgtgcccca
     1561 tagccgtcgc cctttcgaag cgcgacctcg cggatgaaaa ggccaccgca aagtggctct
     1621 cttactttgc cgcatcgggg cagaaggcgt gggcgctcga tctgctcaag ccaagaatgg
     1681 accaggtcag gcgcgatctc gccattttcg gaccggcgca ccgcgaggta cggctggcgg
     1741 ttgtgggcat tccgaacgtc ggcaaatcga tgtttctcaa cgcgcttgta ggaaaatcct
     1801 ccgcgcaggt ggggggcatt cccggaatta cgcgcggcgt ttcatggtac aaagggaagg
     1861 gattcctcgc ggtggattcg cccggaatcc tcgacccgaa agcggacgac gccgtgcagc
     1921 gctgccttgc atggcttgga agcagcaagg cggaagtcat cggagggtat gagaacgtcg
     1981 ctcttgacct tatcgccgtc cttcgccgaa agaacctgtg ggggatggtc gaggccaaat
     2041 ggaatgtcgc cgcacccgct gaaacgtccg acgaagatgt tctcgaggcg ctcgggagac
     2101 gtcttggctg tcttgtttcc ggcggtgcgg tggatctatc gaacgccgca cggcgttttg
     2161 tcgaggcctt ttcgaccggg cgtcttggcc gcgtgacgct ggagcttccg gaggagagct
     2221 gatggaactg aaggagttcg gacgtatcgc cggaaccgac gaggcgggga gaggacctct
     2281 ctgcggaccg gtcgtcgccg ccgcggtcat cctgacggaa acgcagcgcg acgaactgct
     2341 cgcgctcggc ctcgccgact cgaagaagct gtcgccccgg aaacgcgagg cgctcttcga
     2401 caggatgctt tctcacggcg tgctctggcg cgcgcaggcc gcctctcatg aacggatcga
     2461 ccgcatgaac atcctgcgcg cttccctgtg ggcgatgcgg cggagcctga tgaagcttcc
     2521 ccctctcttc gacacggtct tcgtcgacgg cacgttcact ctcc
//
LOCUS       DAOSWW010000352         2559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_31306_length_2559_cov_2.561502, whole genome shotgun sequence.
ACCESSION   DAOSWW010000352 DAOSWW010000000
VERSION     DAOSWW010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31306_length_2559_cov_2.561502"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PK442_07660"
     CDS             complement(<1..882)
                     /locus_tag="PK442_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOO87562.1"
                     /translation="MNSTGKIVAINGPVIRASGMRSFAMREMVQAGRLLLLGEIIRLD
                     GDDALVQVFEDTTGLRTGEPVTGTGGPLSMSLGPGLMGSIFDGIGRPLGKLLEREGPF
                     VTRGVSIPQLDPDAMWDVTPLGKVGDVVTPGAPLALVKETSLVEHRILTPCDIEGEIL
                     SILPPGLHKASSVVARVRDGHGRERSIPMIRKWPVRTPRPIAERLLPDEPLLTGQRII
                     DGLFPISKGGVAAIPGGFGTGKTVTQHQLAKWSDAQIVVYIGCGERGNEMTQVLEEFP
                     VLEDPRSGKPLMERTVLI"
     gene            complement(893..1609)
                     /locus_tag="PK442_07665"
     CDS             complement(893..1609)
                     /locus_tag="PK442_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOO87563.1"
                     /translation="MSNSISGDGRGRELQNPAKRAAEEQNLLEMQRTPVRDDSNRKLL
                     ALKNLLLKKGDEEREVILDSARREATAWLKEQNEGLDRMVEQIHADAVKNAEEIAKRQ
                     IARAESLRAKDRLRLQNTLRDEAMAMLQRALTSLREREDYPLVLAGLVLEAAKDLPAG
                     TAALISLAVDDAPLGDALAERVGKKRPDMTFTFDPSPVPISGGAWLSAPDGSWRAAAD
                     LREVAAELKDSLAERLLAAL"
     gene            complement(1606..1920)
                     /locus_tag="PK442_07670"
     CDS             complement(1606..1920)
                     /locus_tag="PK442_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOO87564.1"
                     /translation="MKGFLISDNHDSLVLLRLAGIPGVVVHGEEETTTAVIDVLRNRP
                     DTGVLVMTERAAAEVPLLVKSLREREDPPLLVEIPDRHGSLREPDFLTRYIRNAIGVK
                     IE"
     gene            complement(1934..2347)
                     /locus_tag="PK442_07675"
     CDS             complement(1934..2347)
                     /locus_tag="PK442_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOO87565.1"
                     /translation="MTGCVLLIAGTLLTCGTGWYLRGKAVRRPRAVLAGFMIGLNALL
                     LSAILVSLSAGSAAAAEGAAAVEGAGLGFLGASLATGMACLGAGLAVAIVGAAALGVV
                     GEKPSMLGTTLIYLGLAEGIAIYGVIVSLLILGKL"
     gene            complement(2410..>2559)
                     /locus_tag="PK442_07680"
     CDS             complement(2410..>2559)
                     /locus_tag="PK442_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOO87566.1"
                     /translation="LLAGNAVIVGLEGLIVFIQTLRLEYYEFFGKFYHGGGIPFEPAT
                     WRSRE"
BASE COUNT          439 a          941 c          752 g          427 t
ORIGIN      
        1 gatcaagacg gtgcgctcca tcagcggctt ccccgagcga ggatcctcaa gcaccggaaa
       61 ctcctccagc acctgcgtca tctcgttgcc gcgctcgccg cagccgatgt acaccacgat
      121 ctgcgcgtcg ctccacttgg cgagctggtg ctgcgtcacc gtcttccccg tgccgaagcc
      181 gcccggaatg gcggcgactc cgcccttcga tatggggaag agcccgtcga tgatccgctg
      241 tccggtcaag agcggctcgt cgggcagcag gcgctccgct atcggacgcg gcgtgcgcac
      301 cggccacttg cggatcatgg ggatgctccg ctccctcccg tgcccgtcgc gcacgcgcgc
      361 gaccacggac gacgccttgt ggagtccggg ggggaggatg gagagaatct ctccttcgat
      421 gtcgcacggc gtcagaatac ggtgctccac cagcgacgtc tctttgacca aagcgagggg
      481 agcgccgggc gtcaccacgt cgccgacctt ccccagcggg gtcacgtccc acatcgcgtc
      541 gggatcgagc tgcgggatcg aaacgccgcg cgtgacaaac ggaccttccc gttccagcag
      601 cttccccagg gggcggccga tgccgtcgaa gatcgagccc atcaggccgg gccccagcga
      661 catcgagagc ggtcctcccg ttccggtcac cggctcgccc gtccggagcc ccgtcgtatc
      721 ctcgaagacc tggaccaggg cgtcgtcccc gtccagacgg atgatctctc ccaggagcag
      781 gagccgtccc gcctgcacca tctcccgcat ggcgaaggag cgcattcccg acgccctgat
      841 cacggggccg ttgatcgcga cgatctttcc ggtgctgttc atctgccgcc cctcacagcg
      901 ccgcgagaag ccgttccgcg agagagtcct tcagctccgc ggcgacttcg cgcagatccg
      961 cagcggcacg ccacgagccg tccggagcgg aaagccacgc gccgccggaa atgggaaccg
     1021 gcgacgggtc gaaggtgaac gtcatgtcgg ggcgcttctt tccgacccgt tccgcaaggg
     1081 cgtcgcccag aggggcgtcg tcgaccgcga gcgaaatcag cgccgccgtc cccgccggaa
     1141 gatccttcgc cgcctccagc accagccccg cgagcaccag tgggtagtcc tcccgctcgc
     1201 gcagcgaggt cagcgccctc tgcagcatcg ccatcgcctc gtcgcgcagc gtgttctgaa
     1261 gacgaaggcg atccttcgcc cgcagcgact cggcccgcgc gatctgccgt ttcgcgatct
     1321 cctccgcgtt cttcaccgca tcggcgtgaa tctgctccac catgcggtcc agcccttcgt
     1381 tctgctcttt cagccacgcc gtcgcttccc gccgcgccga atccaggatg acctcccgtt
     1441 cctcgtcgcc cttcttcagc agcaggttct tcagtgcgag cagcttgcgg ttggaatcgt
     1501 cccgaacggg cgtccgctgc atctcgagaa gattctgctc ctcggccgcc cgcttggcgg
     1561 ggttctgcag ctcccgcccc cttccgtctc cggaaatgga attgctcatt ctatcttcac
     1621 tccaatcgcg ttgcgaatgt accgcgtcag aaaatcaggt tccctcaggc tgccgtgcct
     1681 gtcgggaatt tccaccagga gcggcgggtc ctctctctcc cggagcgatt tgaccagaag
     1741 gggaacctcg gcggccgcgc gttccgtcat gaccagcacc cccgtatccg ggcggttgcg
     1801 caggacgtcg atcaccgcgg tcgtcgtctc ctcctctccg tggaccacta cgcccggaat
     1861 gccggcaaga cgcaacagca ccagagagtc gtggttatcg ctgatgagaa agcctttcat
     1921 cgccggcgcc gcgttacagc ttgcccagaa tcagaaggga gacgatgact ccgtagatgg
     1981 cgattccttc ggcaaggccg aggtagatca gcgtcgtacc cagcatggag ggcttttcgc
     2041 cgaccacgcc gagcgccgcc gcgccgacga tcgccacggc gagtcccgct cccaggcagg
     2101 ccattcccgt cgcgaggctc gcgccgagaa agcccagccc ggccccctcg accgccgccg
     2161 ccccttcggc cgcggcggcg ctccctgcgg aaagggagac aagaatcgcc gacagcagca
     2221 gcgcgttcag accgatcatg aatcccgcga gcaccgcccg gggccgccgc accgccttcc
     2281 cccgcagata ccaccccgtc ccgcaggtca gcagcgtccc cgcgatcaaa agcacacatc
     2341 cagtcataaa aaaccctcct tgaaaagatc gtgtaaaaaa tcgttcggcg ccggaaacgc
     2401 ggcgccctct tactcgcggg atctccatgt cgccggttcg aacggaatgc cgcctccatg
     2461 atagaacttg ccgaagaact cgtagtactc aagccgcagc gtctggatga agacgatcag
     2521 cccttccagc cccacgatca ccgcgttgcc cgccagcag
//
LOCUS       DAOSWW010000353         2548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_31526_length_2548_cov_2.728841, whole genome shotgun sequence.
ACCESSION   DAOSWW010000353 DAOSWW010000000
VERSION     DAOSWW010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31526_length_2548_cov_2.728841"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PK442_07685"
     CDS             complement(<1..591)
                     /locus_tag="PK442_07685"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOO87567.1"
                     /translation="MTTIRRPDTVLGVLGGMGPAAAAEFLRLLAERAPVSSDQEHAIT
                     YLLSDPQVPDRSSAILGVGEDPTARLKNDLLTLTRWGANLLAVPCNTAHFFIDRFKDE
                     LPVPLVHIIEETVHAAAALSPKGAWLTATLGTMGSGLYQKEAERQGYSFFIPPEEAQR
                     QVQRSIELVKSGKKDEGGLLLKGVVEGLWRERDLPVC"
     gene            733..1563
                     /locus_tag="PK442_07690"
     CDS             733..1563
                     /locus_tag="PK442_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOO87568.1"
                     /translation="MNVKKRVWEIVEVAKPGDTVSRVFDIFILTLIIVNVISVIIGSV
                     NQIYDRWGRALDLFEFLSVVIFTGEYVARLWACTVDPRYSGAVRGRIRFALRPMSIID
                     LLAILPFYLPFWSVDLRSMRVLRLLRILRIAKAGRYFTSFGLIKDVLYTNKEELILTT
                     AMMGLLLVVSSSILYYCENEAQPEAFASIPHTMWWAIATLTTVGYGDIAPITGIGRTF
                     ASIIAVIGIGMFALPAGILGAGFVEAIHRRKKREEAAQKKEEDEERGNYCPHCGKRLR
                     "
     gene            complement(1603..2001)
                     /locus_tag="PK442_07695"
     CDS             complement(1603..2001)
                     /locus_tag="PK442_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOO87569.1"
                     /translation="MKKDIRIYPALLSEDGKFVCVRFPDLPGCNTFGEDYADAIASAK
                     DALGGHLLCMEEDNDPIPSPTPVNKLQPEEDEIAVLIDVRMDILREEERKKSVSKNVT
                     IPAWLNKMAIEHKINFSSALQETLRDRLGV"
     gene            complement(2030..2212)
                     /locus_tag="PK442_07700"
     CDS             complement(2030..2212)
                     /locus_tag="PK442_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOO87570.1"
                     /translation="MKSSEIIKILESDGWYWVNTVGSHHHFKHSTKPGKVTVKHPRKD
                     VPPKTFGSIMKQAGLK"
BASE COUNT          532 a          788 c          674 g          554 t
ORIGIN      
        1 gcacaccgga agatcgcgct cgcgccacag cccctccaca acgcccttca gcagcagccc
       61 cccctcgtcc ttcttgccgc ttttcaccag ttcgatgctt cgctgcacct gtcgctgcgc
      121 ctcctccgga ggaatgaaaa acgagtaccc ctgccgttcc gcttccttct gatacaggcc
      181 gctgcccatc gttccaagcg tcgccgtcag ccaggccccc ttcggactca gcgccgccgc
      241 cgcgtgcacc gtttcctcga tgatgtgtac aagaggaact ggaagctcgt ccttgaaacg
      301 gtcaatgaaa aagtgtgccg tattgcacgg cacggccaaa agattcgccc cccagcgcgt
      361 cagcgtcagc agatcgttct tcaagcgcgc ggtcggatcc tctccgacgc cgagaatcgc
      421 gctgctccgg tcgggaacct gcggatccga aagaagatac gttatagcgt gctcctggtc
      481 ggacgaaacg ggagcgcgtt ccgcaagcag ccgcagaaac tccgccgccg ccgcaggtcc
      541 catcccgccg agaaccccca gaaccgtatc cggtctgcgt atggtcgtca taaaaacacc
      601 tccggaaaat atgctccgtg tattatacat ggtatacgtt tttcaggaag agggagcaag
      661 aaaaaactct ccttgactcc tttcgcgcac atgctgtatc tttggcgcac ttcgggagag
      721 gagaggatcg cagtgaacgt caaaaagcgg gtctgggaaa tagtcgaagt cgccaagccg
      781 ggcgataccg tcagcagggt gttcgacatc tttattctta cgctcatcat cgtcaacgtg
      841 atctccgtca tcatcggctc cgtcaaccag atctacgaca ggtggggaag ggcgctcgac
      901 ctgttcgaat tcctctccgt cgtcatcttc accggggagt acgtcgccag actgtgggcc
      961 tgcaccgtcg acccccgcta ctccggcgcc gtccggggac gcatccgctt tgcgctgcgc
     1021 cccatgtcca tcatcgacct gctcgcaatc cttcccttct acctcccctt ctggagcgtc
     1081 gaccttcgct ccatgcgcgt gctgcgcctc ctccgcatcc tgcggatcgc caaggcgggg
     1141 cgctacttca catcgttcgg cctcatcaag gacgtgctgt acacgaacaa agaagaactc
     1201 atcctcacca ccgccatgat ggggctgctg ctcgtcgtct cgtcgagcat cctctactac
     1261 tgcgaaaacg aggcccagcc ggaagccttc gccagcatcc cgcacaccat gtggtgggcc
     1321 atcgccacgc tcactaccgt cggatacggc gacatcgccc cgatcacagg catcggaaga
     1381 accttcgcca gcatcatcgc cgtcatcggc atcggcatgt tcgctctccc cgcgggcatc
     1441 ctgggcgcgg gattcgtcga ggccatccat cggcgcaaga agcgcgaaga ggccgcgcag
     1501 aaaaaagagg aggacgaaga gcggggcaac tactgcccgc actgcggcaa gcgcctgcgg
     1561 tgaggcggga aagccggcta cgctgatcag gggcgatgca cgttacaccc ctagcctgtc
     1621 tctgagcgtc tcttggaggg cgctggagaa attgatctta tgctctatgg ccattttgtt
     1681 gagccatgct ggaatggtga cgttcttgct aacggatttc tttctttcct cttcgcggag
     1741 aatgtccatg cgcacgtcga tcaaaacggc gatttcgtct tcttccggtt gcagcttgtt
     1801 aaccggcgtt gggctcggaa tggggtcgtt atcctcttcc atacagagca aatgtcctcc
     1861 gagagcgtcc ttggcgctgg cgatggcgtc ggcgtagtct tccccgaagg tgttgcagcc
     1921 cggaaggtcc gggaagcgga cgcacacaaa ttttccatct tcggagagaa gcgcgggata
     1981 tatccggata tcctttttca ttgaagtcgt ctcctttccc gaggcgggcc tatttcaacc
     2041 ctgcctgttt catgatactg ccgaaggttt tcggcggaac gtctttccgg gggtgcttca
     2101 ccgttacttt gcccggcttg gtggaatgct tgaagtggtg atggctgcct acggtattca
     2161 cccagtacca gccgtcgctt tcgagaatct tgattatctc gctggatttc atagcatccc
     2221 ctcctgatgc gcaaagtata gtgcgcatta acatgcgcgt caagcgagtt aaaaacggct
     2281 gattggaaaa aaaccaatca catgcggaga aaaaaatgag tctatccgcc gtgcgcagtc
     2341 cagcggaatt acccgaagac gtcgccgaac acgattaccg tcggtacaga tcctccaggg
     2401 tcagaagccg cacgcctccg aagtctctcc aggctttcat cacccctaca aaaactgttc
     2461 tcttgctcta aaggaggaag tggtatcgcg cttttgaaga ctagttttac gcgcattgta
     2521 tgcgcatgat ataataaaaa ccagaaaa
//
LOCUS       DAOSWW010000354         2546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_31567_length_2546_cov_2.985949, whole genome shotgun sequence.
ACCESSION   DAOSWW010000354 DAOSWW010000000
VERSION     DAOSWW010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31567_length_2546_cov_2.985949"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      570..741
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1043..2407
                     /locus_tag="PK442_07705"
     CDS             1043..2407
                     /locus_tag="PK442_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HOO87571.1"
                     /translation="MNLKTKLFGRVYQFKNVKEVLAKANEEKSGDRLAGIIAESMEER
                     IAAKHVLANLTVADLRNNPVIPYEQDEVTRIIQDDVNERIYSEIAQWTLSDLREWILC
                     DETSGDDIKRVSRGLTSEVVAGVAKLMTNLDLVYGASKIRIQAHCNTTIGLPGTLSAR
                     LQPNHPTDDIDGITTSVFEGLSYGVGDAVIGLNPVDDTVDNVKKILFKFQEIKEKFEI
                     PTQMCVLAHVSTQMEAVRQGAPTDLFFQSVAGSQKGNEAFAISGDLLSEAMELAAKQG
                     TGVGPNFMYFETGQGSELSSDSHFGADQVTLEARCYGFAKRFKPFMVNTVVGFIGPEY
                     LYDSRQVIRAGLEDHFMGKLTGISMGCDACYTNHMKADQNDIENLAVLLTSAGCNYFM
                     GVPHGDDVMLNYQCTGFHEAAALRQLLRVRPVAAFEKWLEKMGIMENGILTKKAGDAS
                     IFIR"
BASE COUNT          542 a          727 c          780 g          497 t
ORIGIN      
        1 gaatccgttc aggcgtcgcc acggacgagc cgccgaactt gagtactaca ccttctccat
       61 ctttccacac aaccgccgac ctcccttatg cgtacaaaaa aagccgggag ccttcatcta
      121 cgaagacccc cggctgaagc tatcgagcac gtggtactaa gcgccgaccg ctccaggagg
      181 gtcgccgcta gtcatcgtca ttgcacatgc gaacagaact gccgcgcgga tctttttcgc
      241 attggtcata gtgcggggac ctcctttcga atcggtttag gttgggcagg ataatacgcc
      301 ccttccggtg acttgtcaag cgcaggaagt gaaaaaaatc cgcagggtgg aaaaatgcgc
      361 gcctcccttt cccctctccg tcgctgcaag agcgggcggg gaggggaaat aacgggtggc
      421 aggagtcttg tctgccttta caaggatgtc gatttttaaa ttatatagaa attatttcta
      481 aatggaggac ttctgttgac tctttgtgaa gaaccgttga catttttcta tccatgatat
      541 actctgattg ctggtgaaaa agttgctcta gtttattttt ttgtcgaggt gcgcgcgatg
      601 ctcgggaagc cggttaaaat ccggcgcggg cccgccactg tacgcctgac gaagccgcgg
      661 ggttccgccg gagtcttccg ggaaggagcg gtctgaggat gaaggcaagc caggagaccg
      721 attgcgcgca tcgggcagaa acgccttgcg gggacttgag gccgtttacg gagaagaccg
      781 gcggtgaaca ccggtgtttt tcgcggatgc gccccgggtc tttttttgta cgcgaaattc
      841 cccgggtgcg ggaagcgcgc ggcgccgtat tgctacgaaa ggggggtggt tcgggagagt
      901 tgcactgatg ccgtatgctc ttcgggggcg gtttgttccc ggagacgtat cgccggttgc
      961 gcacgtccgt ccgcgttcct ggccgctctt cggggctccg gcgcgtgagg acgcatgaaa
     1021 acacattgga ggtgaaatga cggtgaacct caaaaccaag ctctttggtc gtgtgtacca
     1081 gtttaaaaat gtcaaagagg ttcttgccaa ggcgaacgag gagaagtccg gcgaccgcct
     1141 cgccggaatc atcgccgagt cgatggagga gcggatcgcg gcgaagcacg tgctcgccaa
     1201 cctgaccgtc gcggatctcc ggaacaatcc ggtgatcccg tacgaacagg acgaagtcac
     1261 ccgtatcatc caggacgacg tgaacgaacg gatttactcc gaaattgcac aatggacgct
     1321 ctccgacctg cgcgagtgga tcctgtgcga cgaaacttcc ggagacgaca tcaagcgggt
     1381 cagccgcgga ctcaccagcg aagtggtagc gggcgtggcc aagctgatga ccaacctcga
     1441 cctcgtctac ggagcgagca agatccgtat ccaggcgcac tgcaacacca cgataggcct
     1501 tccggggacg ctttccgccc ggttgcagcc caaccacccc accgacgata tcgacggcat
     1561 cacaacgtcc gtcttcgagg gcctctccta cggagtgggc gacgcggtga tcggcctgaa
     1621 ccccgtcgac gacacggtcg acaacgtcaa gaaaatcctc ttcaagttcc aggagatcaa
     1681 ggaaaagttc gaaatcccca cccagatgtg cgtcctcgcg cacgtgagca cccagatgga
     1741 ggccgtccgc cagggcgctc cgaccgacct cttcttccag agcgtcgccg gttcccagaa
     1801 ggggaacgag gcgttcgcca tctccggcga tctgctgagc gaggcgatgg agctggccgc
     1861 gaagcaggga acaggcgtcg gcccgaactt catgtacttc gagacggggc agggctccga
     1921 gctgtcgtcc gactcgcact tcggcgccga ccaggtgacg ctggaggccc gctgctacgg
     1981 cttcgcgaag cgcttcaagc cgttcatggt gaacacggtc gtcggcttca tcggccccga
     2041 gtacctgtac gacagccggc aggtcatccg cgcgggattg gaggaccact tcatgggcaa
     2101 gctgaccggc atctccatgg ggtgcgacgc ctgctacacc aaccacatga aggccgacca
     2161 gaacgatatc gaaaacctgg ccgtcctgct cacctcggcg gggtgcaact acttcatggg
     2221 agttccccac ggcgacgacg tcatgctgaa ctaccagtgc accggtttcc acgaggcggc
     2281 ggcgctgcgt cagctgctcc gcgtccgtcc ggtcgccgcg ttcgagaagt ggcttgaaaa
     2341 gatgggaatc atggagaacg gaatcctgac gaagaaggcc ggagacgcgt ccatcttcat
     2401 acggtaggcg ggttctttcc ggttttttcg gaaggggaga ggcatgaaac tgaagactgt
     2461 tcttggagga accgcctggt cgttccgcga tctgaaggac gtgctggcga aggccaatga
     2521 ggagaagtcc ggggatacgc tgctcg
//
LOCUS       DAOSWW010000355         2545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_31585_length_2545_cov_3.655020, whole genome shotgun sequence.
ACCESSION   DAOSWW010000355 DAOSWW010000000
VERSION     DAOSWW010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31585_length_2545_cov_3.655020"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2514)
                     /locus_tag="PK442_07710"
     CDS             complement(<1..2514)
                     /locus_tag="PK442_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOO87572.1"
                     /translation="MESIHWYVVFVLLLVGAVFLVMELLHARAVRKQELFFSDRQYLQ
                     TTMSRLALEDRVSSVLMQERFLLEHLLLEPANGSFPPLSDIQRALQSAIYNTADILGF
                     YFAPHPLYPGAQRFSSFRSTPTGDAAREFAGSLEDSFDFSTPRPDDLYHFSGSVRASS
                     RVRMMALVEAAVSEKGFMGYFITIIDLTPFLVRYVLPMAQGAGSAVFLIDREGVVVEH
                     RDSSLVGGNAEKLSGGEGDALRVAWSRVRESFSGMEAIELFDVSSGMPQRKRLAWNSA
                     RVGENRLFVGVYAPESDINPVVSELRVQRFILGGVLALLLVMIAIAFIRKRSDFEVRR
                     SESTFRAIFNSAASGIAILDANGVILSCNTTMENMTGRPSAQLRMRTLFDLAAPSLQE
                     EKEKVREALRSGALSARAELLFSRKGAEATSPENGSFWGDVSLNRLERDSSTVQMLAV
                     VTDISGLKRAEDLLRQNAETLEARKKELEHVAASQSALLEMFTFFSECVTIEELFQVL
                     SEHLFPVIPFRCQTLFIKRSREAASFEVLDSGGDVLRSMQTDFLEKKKGILGYVANTG
                     KAYLMGDVSIDPYFIPHSPEVRSAVVAPVMYKGFLWGAIVLDSDRKFAFGIRERDMLS
                     IVGSYVALHLEEFFARQELDKKATQLRFLHRVIQQIAAERNNEALARKIVDVLFDELG
                     FPGAGFYTPPPKGENAPVLVAGRSRAERETCSPECELEAGAAMRSRTPVEREVNGRAL
                     VLSIPVTFEGEVFGALAARHDAGFSAADRELLEITAEHMTTFWALNNLLAQRRHESLV
                     DPLTQVWNRRYIMHRLDEESSRINRTEGRGAVILVDLGDF"
BASE COUNT          583 a          773 c          697 g          492 t
ORIGIN      
        1 gaaatccccg aggtcgacga gaatcacggc gcctctgcct tcggtcctgt tgatgcgaga
       61 actctcttcg tccagacggt gcatgatgta ccgcctgttc catacctgcg tcaaaggatc
      121 gacaagcgac tcgtggcggc gttgcgcaag cagattgttc agcgcccaga aggtcgtcat
      181 gtgctctgcc gtgatctcga gcagctcccg gtccgctgcg gaaaaaccgg cgtcgtgccg
      241 ggcggcgaga gcgccgaaga cctctccttc aaaagtgacc ggaatggaga gaacgagcgc
      301 tctgccgttc acttcccgct cgacgggggt gcggcttctc atagcagctc ccgcctcgag
      361 ttcgcactcc ggggaacagg tttctcgctc cgccctcgat ctgccggcaa ccagaaccgg
      421 agcgttctct cccttcggag gaggagtgta gaatccggct ccggggaaac cgagctcgtc
      481 gaagagcaca tcgacaatct tgcgtgcgag cgcctcgttg tttcgctctg cagcgatctg
      541 ctggatcacc ctgtggagaa agcgcagctg cgtcgccttc ttatccagtt cctgtctggc
      601 aaaaaactcc tcgaggtgga gcgcgacgta ggaacccacg atgctcagca tatccctctc
      661 ccgtatgccg aaagcaaact tcctgtcgct gtccagcaca atagctcccc agaggaatcc
      721 cttgtacatc acaggggcga cgacggccga ccgcacctcg ggcgaatggg gtatgaaata
      781 gggatcgatg gacacatcgc ccatgaggta ggccttgccc gtgttggcga cgtatccgag
      841 aatacccttc ttcttctcga ggaaatccgt ctgcatcgac cgcagcacat ctcctcccga
      901 gtcgagcact tcgaacgaag cggcttcacg cgagcgcttg atgaagagcg tctggcagcg
      961 gaacggaatc accgggaaaa gatgttcgga gagcacctgg aaaagttctt ctatcgtcac
     1021 gcactcggaa aagaacgtaa acatctccag cagggcgctt tgactggccg caacatgctc
     1081 aagctccttc ttccgcgctt ccagcgtttc cgcgttttgc cggagcaagt cctccgcccg
     1141 tttcaatccc gaaatatcag tcaccaccgc gagcatttgg acggttgacg agtctctttc
     1201 caggcggttc agcgaaacat ctccccagaa ggatccattc tcgggacttg tagcttcggc
     1261 tcccttcctt gagaaaagga gttcagccct cgccgagagc gcgccggatc gcaaagcctc
     1321 ccgcactttt tccttctcct cctgcaacga cggcgccgcg aggtcgaaga gcgtgcgcat
     1381 cctcaactgc gcggaggggc gccccgtcat gttttccatc gtcgtattac aggaaaggat
     1441 aaccccgttc gcatcgagaa tcgctatccc ggacgcggcg ctgttgaaaa ttgccctgaa
     1501 tgtcgattcg ctccgccgga cttcgaagtc ggaccgcttt cgaataaagg caatagcaat
     1561 cataaccagc aagagcgcga gtacccctcc gagaatgaag cgctgcaccc gcagttcgga
     1621 aaccaccggg ttgatatcgg actcaggagc gtagacgccg acaaacaacc tgttctcccc
     1681 taccctggcg gaattccatg ccagccgttt cctctgcggc atcccggaag agacatcaaa
     1741 aagctcgata gcctccattc cggaaaaaga ctccctgacc cgcgaccacg ccacccgaag
     1801 ggcatccccc tctcctcccg agagcttttc cgcgttgccc cccaccagag aagaatcccg
     1861 atgttcgaca accactccct ccctgtcgat gaggaagacc gcgctccctg ccccctgggc
     1921 catggggagc acatatctga caaggaaagg ggtaagatcg atgatcgtga tgaagtaccc
     1981 cataaagcct ttttccgata cagccgcctc aaccagcgcc atcatcctga cgcgcgagga
     2041 cgcccggacg cttcctgaaa aatggtagag atcgtcggga cgaggagtgg aaaaatcaaa
     2101 agaatcttcc agagaaccgg caaattccct ggctgcgtct ccggtcgggg tcgaacggaa
     2161 cgaactgaac cgctgcgcgc cggggtacag cgggtgagga gcaaaataga atcctaggat
     2221 gtcggcggtg ttgtaaatgg cgctttgaag cgcgcgttga atgtcggata gaggagggaa
     2281 ggacccgttt gcaggctcta gaagaaggtg ctccaaaaga aaccgttctt gcatgagaac
     2341 ggaggagacc cgatcctcta acgcgagacg cgacatggtg gtttgaaggt actgacggtc
     2401 gctgaaaaac agttcctgct tgcggactgc gcgggcatgg agcagctcca tgaccaaaaa
     2461 tacagcccct acaagcagca agacgaaaac cacgtaccag tgaatagatt ccatgcgtcc
     2521 ggtacgcttc ggagaatcgg cgttc
//
LOCUS       DAOSWW010000356         2535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_31795_length_2535_cov_3.129435, whole genome shotgun sequence.
ACCESSION   DAOSWW010000356 DAOSWW010000000
VERSION     DAOSWW010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31795_length_2535_cov_3.129435"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK442_07715"
     CDS             <1..483
                     /locus_tag="PK442_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87573.1"
                     /translation="GLELAHEYHLPRVIRSFIAEHHGTTALAYFLRKARNMGLTIPRE
                     QFCYPGPKPQSRETGLMMLVDSVEAAVRAESRAGSESILDVRDTINRVIDSKITEGQL
                     DDVDFTLRDLSVIRETLLSALRTVYHTRKVKEIQEKESPQHTTEKQEDDVSEASVASR
                     "
     gene            458..988
                     /gene="ybeY"
                     /locus_tag="PK442_07720"
     CDS             458..988
                     /gene="ybeY"
                     /locus_tag="PK442_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOO87574.1"
                     /translation="MKLQLRLDSSEDADPASTAFSYLVERAEPAFTRALCDLFPEGTL
                     PAKVEISLSFLSLGEMKAQNRKYRGLDEPTDVLSFPLWEEDGVFRPGRSLPTLPLGDI
                     LICPEYVRAAVSSEEEFQREMALMLAHGFLHLLGWDHDTDERRSAMEVRQELIRKELL
                     AFPEGAASGTAEEELR"
     gene            985..2289
                     /locus_tag="PK442_07725"
     CDS             985..2289
                     /locus_tag="PK442_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOO87575.1"
                     /translation="MSSLVAQSAALLLVLLFLSAFFSASETAITSSGRGKILALIERY
                     PYQKRFFEWLLKDVQRALTIVLISNNLVNIAASAVGTTLAIATIGQGGVLLAVPLMTA
                     LIVIFGEVFPKSVAIIQSDFVLVFSLPFLRLLDILLAPFVWLMIAAVRLLGLLFKVDL
                     KARHPFVTREEFEQMVNIGEESGALEEVERRMIHGIISFEETRVYEIMVPRTDMDAVP
                     SNCTVAEAMKVFQEHGHSRVPVFEENLDDIVGILYVKDTIPNLLAGELETPVSSIMRN
                     ALFVPESMRVVELFNNMKGKHVHMAIVVDEYGGVAGIATLEDLLEEIVGEIQDEYDKE
                     KPSFTPEEDGSYLVQGHVSLEDLSDLLEASFESEDAESLGGLVLSISGDFPSPGEKIH
                     YRSPDGAGNWEIEVLEVEDHRIKLLRLLPKERGYFIPPEVVE"
     gene            2286..>2535
                     /locus_tag="PK442_07730"
     CDS             2286..>2535
                     /locus_tag="PK442_07730"
                     /inference="COORDINATES: protein motif:HMM:NF019818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87576.1"
                     /translation="MTLFTSPVSWPASWPSPQKLLDAAREAQRASYSPYSGFPVGAAL
                     FVEGMDDLILACNVENGSYGLSICAERSAAVAMIARGGK"
BASE COUNT          526 a          728 c          745 g          536 t
ORIGIN      
        1 gggctggagc ttgctcatga ataccacctg cccagggtta tccggtcgtt catcgcggag
       61 caccacggaa ccaccgcgct tgcgtacttc cttcggaagg cgagaaacat gggactcact
      121 attccccggg agcagttttg ttacccgggt ccgaaaccgc agagcaggga gaccgggctg
      181 atgatgctgg tcgattcggt ggaggcggct gtccgggcgg aatccagagc aggttccgag
      241 tcgatactcg acgtccggga tacgatcaac cgggtgatcg actcgaaaat caccgaagga
      301 cagctcgacg acgttgattt taccctgagg gatctctcgg tgatccggga gacattgctc
      361 tcagcgctgc ggacggtgta tcatacccgc aaagtaaaag agatacagga gaaagagtct
      421 cctcaacaca ccacagaaaa acaggaggac gatgtcagtg aagcttcagt tgcgtctcga
      481 tagcagcgag gacgccgatc ccgcaagtac cgcgttcagc tatctcgtag aaagggcgga
      541 gccggcgttt acgcgcgccc tttgcgatct ttttccggag gggactctcc ccgcgaaggt
      601 cgagatatcc ctgtcctttc tctctctcgg cgaaatgaag gcacaaaacc ggaaataccg
      661 cggactggac gaaccgacgg acgtcctctc cttccccctt tgggaagagg atggagtctt
      721 ccgtccgggg cggtcgctcc cgacgcttcc tttgggcgat attctgatat gcccggagta
      781 cgttcgcgcc gccgtctctt cggaagagga attccagaga gaaatggcgc tgatgcttgc
      841 tcacggtttc ctccacctgc tcgggtggga tcacgatacc gatgagcgga gaagcgctat
      901 ggaagtacgg caggagctga tacggaaaga actgctggcc tttcccgaag gtgcggcttc
      961 cgggaccgcg gaggaggagc tccggtgagt tctcttgtag cgcagtcggc ggcgcttttg
     1021 ctggtactgc tcttcctatc tgcctttttc agcgcctctg aaacagcgat cacatcctca
     1081 ggcagaggaa aaatccttgc gctgatagaa cggtatccgt atcagaagcg ttttttcgag
     1141 tggctcctca aggatgtgca gcgtgcgctc accatcgtct tgatttcgaa caatctcgtc
     1201 aacatagccg caagcgcggt cggcacaact ctggcgattg ccacgatagg tcagggaggc
     1261 gtcctgctgg cagttccgct gatgacggcg ctcatagtga tcttcggcga ggtgtttccg
     1321 aagagtgtcg ctataataca atcggatttc gtgctcgttt tttcgctccc gtttttgagg
     1381 ctgctggata tcctcctcgc ccccttcgta tggctcatga tcgcggcggt ccggctgctc
     1441 ggtcttctct tcaaggtgga cctgaaagcc cggcacccct tcgtaacgag agaggaattc
     1501 gagcagatgg tcaacatagg ggaggaatcc ggcgcgctcg aagaggtgga gcgccggatg
     1561 attcacggca tcatctcgtt tgaggagacc agggtctacg agatcatggt cccgcgcacc
     1621 gacatggacg ccgtgccttc aaactgcacc gtcgcggagg ccatgaaggt ctttcaggag
     1681 cacggacact cccgcgtccc cgttttcgaa gagaacctcg acgacatcgt cggcattctc
     1741 tacgtcaagg acactattcc gaacctcctg gccggggagc tcgaaacccc cgtctcctcc
     1801 atcatgagga acgcgctctt cgtgcctgag agcatgcgcg tcgtcgaact cttcaacaac
     1861 atgaagggga aacacgtcca catggccatc gtcgtcgacg agtacggcgg cgtggcaggc
     1921 atcgccaccc ttgaagacct ccttgaggag atcgtcggcg agatacagga cgaatacgac
     1981 aaggaaaaac cttcctttac ccccgaagag gacggcagtt acctcgttca ggggcacgtc
     2041 agcctggagg atctcagcga ccttctggaa gcgtcgttcg aatccgagga cgccgaaagt
     2101 ctcggggggc tggtgctctc catatcggga gattttccct ccccgggcga gaagatacac
     2161 taccgctcgc cggacggcgc aggcaactgg gagattgaag ttcttgaagt ggaagaccat
     2221 cgaattaagc tcctccgtct ccttccgaag gagcgggggt attttatccc accggaggtt
     2281 gtcgaatgac cctgtttacg tcgcccgttt cctggcctgc atcatggccg tcgcctcaaa
     2341 agctccttga cgcggcgcgt gaggcgcagc gcgcatcata ctccccctac tcaggcttcc
     2401 ctgtcggggc ggctctgttc gtagagggta tggacgatct tatccttgcg tgcaacgtgg
     2461 agaacggctc ctacggactc tccatctgcg ccgagcggag cgccgccgtc gcgatgattg
     2521 cccgcggagg caaaa
//
LOCUS       DAOSWW010000357         2530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_31910_length_2530_cov_3.637980, whole genome shotgun sequence.
ACCESSION   DAOSWW010000357 DAOSWW010000000
VERSION     DAOSWW010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31910_length_2530_cov_3.637980"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="PK442_07735"
     CDS             complement(<1..1748)
                     /locus_tag="PK442_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO87577.1"
                     /translation="MDLLLIKSHRSVCGYIPGSNLGMNILIQLLRQKKYTAEMFQGYA
                     KEALSHVKMLMEQEDRPKVIGFYCDFDNVHWVRLFAREVKRNYPEVILLAGGPQTVDM
                     DADFLKDTGILAVCIGEGEETVPELMEYCIHRRGSLETIQGIAFLDASSQLVKTPPRT
                     PPEALDSIPWPDITLTNDYKHYSLLPILTGRGCPFGCTFCYEGANAKRVRRHSVPSLL
                     HEIRANFERNPSIKYINFLDDTFTLDHKRVDEICEGIKELRKEYDFVWFASAHISLIG
                     KHRDMARTMAEAGLKKIFFGLESGSDAVLQSYNKKITRKMAVEVIAHCVDSGIHAISG
                     NIILGGPHETRETVQESEDLIMELLYRAPGQFETAYFSFLPFPQTPITLNPQKFGMEI
                     YESLIDCCNEDIPLSGTEALDFIELTKIRLQANKRLQNEMRTIYLDGKIPESVILDAY
                     RLGERYGVFTRWNDYVYKNLPIDDAYWRMRAFGEYVTGSQLGAWEENAIPHRTFELWL
                     YTDISGAKPRVVDTPLEEIDYTLLKLCSGKLSKREVIRQGKAALDPAGTQPDFYKRAE
                     EALHSMEQKKWILYQKP"
     gene            complement(1833..2096)
                     /locus_tag="PK442_07740"
     CDS             complement(1833..2096)
                     /locus_tag="PK442_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HOO87578.1"
                     /translation="MDGAKALAAKVLTDEELAKKLGEAKSEAEFNAVVAKLGYKCTAA
                     EFEAAFKEAQGKAPLSDEQLDQASGGLSIVGVDYAFVAVQTAK"
BASE COUNT          544 a          668 c          636 g          682 t
ORIGIN      
        1 ggtttctggt agagaatcca tttcttctgt tccatcgaat gcagcgcttc ttccgcccgt
       61 ttgtaaaagt cgggctgcgt tcccgcgggg tcgagcgccg ctttcccctg ccgaatgact
      121 tcccgtttcg agagcttccc gctgcataat ttgaggagcg tgtagtcgat ttcttccagc
      181 ggcgtatcga caacccgcgg ttttgccccg ctgatgtcgg tgtacagcca caactcgaag
      241 gtccggtgcg gaatggcgtt ctcttcccaa gcgcccagtt gactgcctgt gacatactcg
      301 ccgaacgccc gcattctcca gtaggcgtcg tcgatgggca ggtttttgta tacatagtcg
      361 ttccatcggg taaatacgcc gtagcgctcc cccagacggt atgcgtccag aatcaccgat
      421 tcgggaattt tgccgtcaag gtagattgtt cgcatttcat tctggagcct cttgttggcc
      481 tgaaggcgta tctttgtaag ctctatgaaa tccaatgctt ccgttccgga aagcggaata
      541 tcttcgttgc agcagtcgat gagggactcg taaatttcca tcccgaattt ttgaggattc
      601 agggtgatcg gcgtttgagg aaacggaaga aaggagaagt aggcggtttc aaactgcccg
      661 ggcgccctat acagcagctc catgatcaga tcttcggact cctgaacggt ttccctggtt
      721 tcgtgaggcc ctcccagaat aatgttgccg gaaatagcgt gaatccccga gtcgacgcag
      781 tgggcgatga cctccaccgc catcttccgc gtaattttct tgttatagct ttgcaaaacg
      841 gcgtcgctgc cggactccag gccgaaaaaa atttttttca gccccgcctc cgccatagtg
      901 cgggccatgt cccggtgttt gccgatgagg gagatatgag cgctggcaaa ccatacgaaa
      961 tcgtactctt tgcggagttc cttgattccc tcgcagattt catcgacccg tttatgatcc
     1021 agcgtaaagg tgtcgtcgag aaaattgatg tacttaatgg aggggttccg ttcaaagttt
     1081 gccctgatct cgtgaagcag cgagggaacg gagtggcggc gcacccgttt tgcattggcc
     1141 ccctcatagc agaaggtgca tccgaaagga cagccccgtc cggttaaaat ggggagaagc
     1201 gagtagtgtt tatagtcgtt ggtcaaggtg atatcaggcc aaggaatgga atccaaggct
     1261 tcaggcgggg ttctcggggg tgttttcaca agctgtgaag aagcatccag aaaagctatt
     1321 ccctgaattg tctccagtga ccctcggcga tggatacaat actccatgag ctccggaact
     1381 gtttcttccc cttcgccgat gcagacggca aggattcccg tgtctttcaa aaaatcggcg
     1441 tccatgtcga cggtttgcgg cccgcctgcc agcagaatca cttccgggta gtttcttttc
     1501 acttcccggg cgaagagcct gacccagtgg acattgtcaa aatcgcaata gaatccgatg
     1561 actttgggcc ggtcttcctg ctccatgagc atttttacat ggctcaaggc ttctttggca
     1621 tagccctgaa acatctcggc tgtatatttt ttttgccgta aaagctgtat taaaatattc
     1681 attcccagat tgcttccggg aatgtacccg cagacagagc ggtgtgattt tatgagcaac
     1741 agatccacgt ggtttcctcc gcctaaaaga ggagaccgga aaaattccgg cctcctcttt
     1801 catcatccgt gaaggagaag gggctccttc gtttattttg ctgtttgcac agctacaaac
     1861 gcgtaatcca cgccgactat gctcaaaccg cctgaagcct gatccaactg ctcgtcgctg
     1921 agcggcgcct tcccctgggc ttccttgaag gcagcctcga attccgccgc agtacacttg
     1981 taccccagtt ttgcaaccac cgcattgaat tccgcttcgg atttcgcctc tcctagtttc
     2041 ttcgccagct cctcgtccgt tagaaccttt gcagccaatg ctttcgcgcc gtccatgatt
     2101 ttcttctcct ccttgtgtgt ctataaggta ggtgatacgc acctcgtatg aaaatataga
     2161 aattattata cagcccaccg ggatattgtc aaatgagctc tttggggaga ccttgtcaga
     2221 agttgcgatg cgcccgaaac acggaagagc ggcgtcgttg tggcggcgat acagtctgtt
     2281 ccattgaatg aactcctctt gagacaagtt gtttcaaatg tcaccgacag gtaacaaaaa
     2341 tctcttccgc gtgatataat gcgaacgact tttttccgca aggagttttc tgcaaacata
     2401 atctcctgac aaaaggagtg gtataggttg cgacattctc ttcgtatcct cagcgtttcg
     2461 tttcttcttc tttttcttac cagttcgtcg ctcttcgggg cgggattcgc gctgtatgac
     2521 ttcagcgcgc
//
LOCUS       DAOSWW010000358         2519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_32144_length_2519_cov_2.848620, whole genome shotgun sequence.
ACCESSION   DAOSWW010000358 DAOSWW010000000
VERSION     DAOSWW010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32144_length_2519_cov_2.848620"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PK442_07745"
     CDS             complement(<1..527)
                     /locus_tag="PK442_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOO87579.1"
                     /translation="MGKKVREGVFVYSVVLVMLLFAAQAQAGGVRLSPLNPEFAGASA
                     LSAGASAGAAQIRTPWGDVLPVGRRPSPLNLEHLKHPEAGASARTKRALPAKYDLRTL
                     NRLSPIRDQDPYGSCWTFGAMAAAESPLLPVEVRDFSEWHLGYWAYMTNPEGFPAFTQ
                     WSDPANPFPDERARI"
     gene            complement(663..1202)
                     /locus_tag="PK442_07750"
     CDS             complement(663..1202)
                     /locus_tag="PK442_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOO87580.1"
                     /translation="MAGLDPNMVYGLVTGLLFGFLLQRGRVIRYDKQLGALRVQDFTI
                     LKFMLSSIVVAMVGVYILLDMGMVKLSIKPMIAGGVVVGGLLFGVGWGLLGYCPGTSV
                     AAVGEGRLDALWGIAGGLVGAALYAEAFPWVSRTLLTMGDYGKITIPQALGMNHWYVI
                     GGAAVLTLLFFMVSEKKGL"
     gene            complement(1183..1716)
                     /locus_tag="PK442_07755"
     CDS             complement(1183..1716)
                     /locus_tag="PK442_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1324..1326),aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOO87581.1"
                     /translation="MKEEGGWNPYLAGGLSGLVSIGSVWFAGKYLGASTTFVRATAAV
                     EQAVLPERAATLEYLVKTAPKMDWQAWFLVGIFIGSLVAAALFGDFKFELVPPMWKKR
                     FGDGVLKRGVVAFIGGVVAMFGARLADGUPSGHGLSGSLQLAVSGIVAFAAFYIAGMV
                     TAKLVYGGGERNGGTGS"
     gene            complement(1860..>2519)
                     /locus_tag="PK442_07760"
     CDS             complement(1860..>2519)
                     /locus_tag="PK442_07760"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOO87582.1"
                     /translation="IAAARQGKKVGILDADITGPSIPTFFGLHTFPFGNADGIEPPAS
                     RSGIRVISMNLLLDDPAKPVIWRGPLLGNAVTQFFNDVQWGELDVLFVDLPPGTADAV
                     LTVMQTIPLDGFLLVTSPQELASLVVRKAAKMAEMLDIPLVGVVENMSSLRCPHCGER
                     IELFGGSHAEALINDVKATFWGSLPLDTEMAALGDEGHLEEYTNTEVFSVLEKGLANL
                     L"
BASE COUNT          551 a          834 c          710 g          424 t
ORIGIN      
        1 tagattcttg cccgctcgtc cgggaaggga ttcgccgggt cgctccattg ggtaaatgcg
       61 gggaagcctt ccgggttcgt catatacgcc cagtacccca ggtgccattc ggagaaatcg
      121 cgaacttcga cgggcagcag gggggattcc gcagccgcca tagcgccgaa ggtccagcag
      181 gaaccgtacg ggtcctgatc gcgaatcgga gaaagcctgt tcagcgtccg caggtcatac
      241 ttcgcaggga gagccctctt cgtccgcgcg gacgcccctg cctcaggatg ctttagatgc
      301 tccaggttca gcggcgacgg ccgtcttcca acaggcagca catcacccca gggcgtgcga
      361 atctgcgcag ccccggcgga ggctcccgcg gagagcgcgg acgcccctgc aaactccgga
      421 ttcagcggcg acaagcgaac tcctcccgcc tgcgcctgcg ctgcaaagag cagcatgacg
      481 agaacaacag aatacacgaa aaccccttca cgcacttttt tccccataaa ctccccgtcc
      541 tttcaaattc aactccacac ccataaagca tacactcttt tcggccggca gggaatgaaa
      601 aaagcggcct ccggaaaagt ttatccggag accgctcggg gtgcaaagca aaaaacgtct
      661 ttctatagcc ccttcttctc cgaaaccatg aagaaaagaa gcgtgaggac cgcagctccg
      721 ccgatgacgt accagtggtt catccccagc gcctgcggaa tggtgatctt tccgtagtct
      781 cccatcgtga ggagcgtccg gctcacccac ggaaaggcct cggcgtaaag cgccgcgccg
      841 acgaggccgc ccgcgatccc ccagagggcg tcgagacgcc cctccccgac cgccgccacg
      901 gaagtgccgg ggcagtaacc cagcagcccc cagccgacgc cgaagagaag gccgccgacc
      961 acgacgccgc ccgcgatcat gggctttatg gaaagtttca ccattcccat gtcaaggagg
     1021 atgtagacgc cgaccatcgc gaccacaatg gacgacagca taaacttcaa tatggtgaag
     1081 tcctgcacgc gaagggcgcc gagctgcttg tcgtaccgga tcacgcgccc cctctgcagc
     1141 aggaagccga agagaagccc cgtgacgagg ccgtaaacca tattaggatc cagtcccgcc
     1201 attgcgctcg cctcctccgt acacaagttt cgccgtaacc atgccggcga tgtagaacgc
     1261 cgcaaacgcg acgatgccgc tgaccgcaag ctggagcgaa ccgctcagcc cgtgcccgct
     1321 gggtcacccg tccgcgaggc gcgcgccgaa cattgcgacg acgccgccga tgaaggcgac
     1381 aaccccgcgc ttcaacacgc cgtcaccgaa ccgcttcttc cacatcggcg gaacgagctc
     1441 gaacttgaag tcgccaaaaa gtgcggccgc aaccagcgaa ccgatgaaaa tgcccacaag
     1501 gaaccacgcc tgccagtcca tcttcggggc cgtcttcacc aggtattcaa gcgtcgccgc
     1561 ccgctccgga agcaccgcct gctccaccgc cgcagtcgcc cggacaaagg ttgtggacgc
     1621 ccccagatac ttcccggcga accagaccga gcctatggaa acaagcccgg acaatcctcc
     1681 ggcgaggtag gggttccatc cgccctcttc tttcatcgta tcccctccat tctccagata
     1741 atattacaaa tactgcactt ccttacaatt taattatatc tgcttatatt gcattgtcaa
     1801 cctttcacat aaaaaaaaga gccccggagg gctcttcact cttcttgccg gagagagcgc
     1861 tacaacaggt tggcaagccc cttctccaac acggagaaga cttcggtgtt ggtgtactcc
     1921 tcaaggtgcc cctcgtctcc cagcgcggcc atttccgtat ccagaggaag gctcccccag
     1981 aaggtggcct tcacgtcgtt gatcagcgcc tcggcgtggc ttccgccgaa aagctcgatc
     2041 cgctcgccgc agtgcgggca gcgaaggctg ctcatgttct ccaccacgcc gacgagggga
     2101 atatccagca tctccgccat cttcgcggcc ttgcggacca cgagggaggc aagctcctgc
     2161 ggcgaggtca ccagcaggaa gccgtcgagc gggatagtct gcatcaccgt gagcacggca
     2221 tcggcggtgc cgggaggaag gtcgacgaag agcacgtcga gttcgcccca ctggacgtcg
     2281 ttgaagaact gggtcacggc gttcccgagg agagggcccc gccagatcac cggcttcgcc
     2341 ggatcgtcga gtagcaggtt catcgagatg acgcgaattc cggaacgcga cgcgggcggt
     2401 tcgatgccgt cggcgttgcc gaaggggaaa gtgtgcaggc cgaaaaacgt ggggatggac
     2461 ggcccggtga tgtcggcgtc caggatgccg accttctttc cctgacgagc ggcggcgat
//
LOCUS       DAOSWW010000359         2514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_32249_length_2514_cov_3.252135, whole genome shotgun sequence.
ACCESSION   DAOSWW010000359 DAOSWW010000000
VERSION     DAOSWW010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32249_length_2514_cov_3.252135"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PK442_07765"
     CDS             complement(<1..853)
                     /locus_tag="PK442_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOO87583.1"
                     /translation="MLLFPAAEYAERLAKTKERMHEHGVEVLIVSHPANMNYLTGYDG
                     WSFYVHQGLIVSLDCDEPIWFGREQDGNAARILTSLKEENIKGYPDHYVMSKLRHPMQ
                     FVADILRERGWDARHVGVEMEGYYFTAEGFQTLRRELPKGVVKDGTLVVNLVRRIKSA
                     REIEYVRQAARIAENVMKTALDVIAPGVRENDAAAEVYKAQIAGTPEFGGDYTSLCPI
                     MPSAERTSSAHLTWCADRKYREGDIVLLELAGARHHYHCPISRTLFLGEPPAELRKTA
                     DAVVKGLN"
     gene            1195..1686
                     /locus_tag="PK442_07770"
     CDS             1195..1686
                     /locus_tag="PK442_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87584.1"
                     /translation="MKKLFALAAVLALALLVSASAFAGTFTLPKAGIDFTLPDSWQPS
                     DSKLGTMFVSPGSDMVIFFGETNSEAGLDGAMELVEQVVSEVLTDIELGEDTEHDING
                     IQVFALEGTGKSDGANAFVSISILNAEGDSLGIMVSFGDEGADQKNAADLQALVGSLK
                     IAQ"
     gene            complement(1771..2373)
                     /locus_tag="PK442_07775"
     CDS             complement(1771..2373)
                     /locus_tag="PK442_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87585.1"
                     /translation="MKRVPVVLSLLVLLLFASCAQAALVELKPGADELKRMSVFLSNF
                     TEVGFFEIEDVSWLTGDDLVYFGIRHNYINNFKSRIRPCKVKNCPYGSMTIEAKWVAE
                     SVKKYFDIDFKQHDDAEEPLKAHYDGKLYHFDGSDGDVVYYAQVQTVARDGNVFHFKG
                     HLYNVSDKKEIPALFRATAKAHTFGGKRTWALLSIETVWN"
BASE COUNT          465 a          758 c          732 g          559 t
ORIGIN      
        1 cgttcaggcc cttgacgacg gcgtccgctg tcttccgaag ctccgcgggc ggctccccga
       61 ggaagagcgt gcgcgatatg gggcagtgat agtgatggcg cgcccccgca agttcgagga
      121 ggacgatgtc cccctcccgg tacttcctgt cggcgcacca ggtgaggtgt gccgacgagg
      181 tgcgctcggc cgacggcatg atcgggcaga gcgaggtgta gtcgccgccg aactccggcg
      241 tgcccgcgat ctgcgccttg tagacctcgg cggccgcgtc gttctcgcgg actccggggg
      301 cgatgacgtc gagcgccgtc ttcatgacgt tctcggcgat gcgcgccgcc tgccggacgt
      361 actcgatctc gcgcgccgac ttgatcctgc ggacgaggtt gaccacgagc gtcccgtcct
      421 tcacgacacc cttcggaagc tcgcgccgga gcgtctggaa gccctcggcg gtgaagtagt
      481 acccctccat ctcgacgccg acgtggcgcg cgtcccatcc gcgttcgcgg aggatgtcgg
      541 cgacgaactg catcgggtgg cgcagcttcg acatgacgta gtggtcgggg tagcccttga
      601 tgttctcctc tttgagggag gtgaggatgc gcgcggcgtt gccgtcctgt tctcgcccga
      661 accagattgg ctcgtcgcag tcgagggaga cgatcaggcc ctgatggacg tagaaggacc
      721 agccgtcgta cccggtgagg tagttcatgt tggcggggtg ggagacgatc agcacttcca
      781 ctccgtgctc gtgcattcgt tccttcgttt tcgcgaggcg ctccgcgtac tctgcagccg
      841 gaaagagcaa catcttgtca ccgccttgtg gaagttttcg tttttatgtc gtttgtcgac
      901 aataaaacaa taccccgtaa aaagcggttt gtcaagacgc gggagcccgt gtattttttt
      961 tgtatgtaac aaaagttttt cttgccaaag ttcgcttccc gtgctattct tccgcttgta
     1021 ctgtgttttt gtacgcgttt cctgttttct cttgaactga tgcttcaagt atatttgaat
     1081 cgagtctatg tatcttttgc gtaaaaaatg gaagaacatg gaaggaagca gcttcgtgtc
     1141 ttctcgtttg agcagcacat cataatatca ttcacaaggg ggattcaccg taccatgaaa
     1201 aagcttttcg cacttgcagc tgttcttgct cttgcgcttc tcgtctccgc gtcggcgttc
     1261 gccggaacct tcaccctgcc gaaggccggg atcgacttca cgcttcccga ttcatggcag
     1321 ccttccgact ccaagctcgg cacgatgttc gtctctcccg gcagcgacat ggtcatcttc
     1381 ttcggtgaaa cgaacagcga agcgggactc gacggcgcga tggagctcgt cgagcaggtt
     1441 gtgagcgaag tcctgaccga catcgaactc ggcgaggaca ccgagcacga catcaacggc
     1501 atccaggtct tcgccctcga gggaaccggc aagtccgacg gcgcgaacgc cttcgtcagc
     1561 atcagcatcc tgaacgccga aggcgactcg ctcggcatca tggtctcctt cggagacgag
     1621 ggcgccgacc agaagaacgc cgccgacctt caggctcttg tcggctcgct gaaaatcgcc
     1681 cagtaagaac taaaagcaca cggaaacagc gaaggaggag ccgcgaaggc tcctcctttt
     1741 ttatgccgta ctccaccctc cggggagggg ttagttccag acggtctcta tcgagagcag
     1801 cgcccaggtc ctctttccgc cgaaggtgtg cgctttcgcc gtggcgcgga agagggccgg
     1861 tatctccttc ttgtcggaga cgttgtacag atgccccttg aagtggaaga cgttcccgtc
     1921 gcgggcgacc gtctgtacct gcgcgtagta gaccacgtcg ccgtcggatc cgtcgaagtg
     1981 gtagagcttg ccgtcgtagt gcgccttcag cggctcctcc gcgtcgtcgt gctgcttgaa
     2041 gtcgatgtcg aagtattttt tcaccgactc cgccacccac ttcgcctcga tggtcatcga
     2101 cccgtagggg cagttcttca ccttgcaggg gcggatccgg ctcttgaagt tgttgatgta
     2161 gttgtgccgg atgccgaagt agaccagatc gtcccccgtc agccaggaga cgtcctctat
     2221 ctcgaagaaa ccgacctccg tgaagttgct caagaagacg ctcatcctct tgagttcgtc
     2281 ggcgcccggc ttcagctcga cgagcgccgc ctgtgcgcag gacgcgaaca gcagcaatac
     2341 aagcagcgag agaacgaccg gtactctttt catgacacgc acctccctga gttatgtaac
     2401 ctccgctccc cggctacctt gaatagacgc cgtcgagaag aacgccctga ccgcagaatc
     2461 cgctctcctt gaaagcgtcg tccgtgctca caaccgcctt tttcccgtcg aaga
//
LOCUS       DAOSWW010000360         2514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_32254_length_2514_cov_2.889386, whole genome shotgun sequence.
ACCESSION   DAOSWW010000360 DAOSWW010000000
VERSION     DAOSWW010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32254_length_2514_cov_2.889386"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PK442_07780"
     CDS             <1..509
                     /locus_tag="PK442_07780"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868776.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOO87586.1"
                     /translation="ITTDHILPAGSRIMSLRSNLPAISEYCFNIVDPSFPERARNAGG
                     GILVAGENYGQGSSREHAALSPLFLGVKAIITVSYARIHRQNLVNAGILPLLFEDRAD
                     LEKIAQGDRLELVNLHSCLEGEFVEVRNVTTGACIRTRHGLSRRQREILLAGGALNAA
                     RVALTKEP"
     gene            531..1610
                     /locus_tag="PK442_07785"
     CDS             531..1610
                     /locus_tag="PK442_07785"
                     /inference="COORDINATES: protein motif:HMM:NF019824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HOO87587.1"
                     /translation="MFGLEERALVSPREMEERSRLLASRGLTVPGNESVILGFFEEER
                     LVATGSLVGNILQGIAVDRELEGEGVAATVTSALLKKAMQMGRRQLFLYSKPDEAKRF
                     EALGFTLLATASVERTGLGASLLEWGSEGIDKWRAAIATKAEGGPEGAGVAVVNCNPF
                     TLGHYCLLEYAAARTPRLYVLVVEEDRSLFPFEVRFRLVKEGVSELKNVVALRGGPYV
                     ISSATFPSYFTRIESGEEERITELYAALDIELFGRYVASALKATERFVGTEPYCPVTS
                     IYNRMMKERLPKGGEGWPPVRVHEMPRFEMEGMPVSASRVRSLIREDRMEDVRPLVPE
                     STWKWLVSDEALPVIEKIRNSESRH"
     gene            1649..1918
                     /gene="citD"
                     /locus_tag="PK442_07790"
     CDS             1649..1918
                     /gene="citD"
                     /locus_tag="PK442_07790"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOO87588.1"
                     /translation="MNGVKNSVAGSLESSDVLVTVGPAEGAENTLDVESIVFKQFGAR
                     IRSVAEEVMTASGVRGADVRIQDRGALECTLRARLETALERALKA"
     gene            1918..>2514
                     /locus_tag="PK442_07795"
     CDS             1918..>2514
                     /locus_tag="PK442_07795"
                     /inference="COORDINATES: protein motif:HMM:NF015742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HOO87589.1"
                     /translation="MTSPLDARETRWRKRIDLASALPPGCSLLSFTLRMPAPLRLGGE
                     YDRTAGEWFDELRQFLAKEGMSAPSYDFYVSADGPEGFCTVRGEGKDVKRRAVAFEET
                     HPCGELVDVDVTCPDGSVPGRRELGLPPRRCVVCGGEAVPCVRGRAHTAEELRSAVER
                     IAEGWRACRRERRSGLNAPASDERSARGVGEGRTFGTCN"
BASE COUNT          442 a          710 c          921 g          441 t
ORIGIN      
        1 acatcaccac ggaccacatc ctccctgcgg gcagtcggat tatgtcgctg cgctcgaatc
       61 tcccggctat ctcggaatac tgtttcaaca tcgtcgatcc ctccttcccg gagcgagcgc
      121 gcaacgccgg aggaggaatc ctcgtcgcgg gggagaacta cgggcagggg tcgagcaggg
      181 agcatgcggc cctctcgccg ctctttctcg gcgtcaaggc gatcatcacc gtctcgtacg
      241 caaggatcca caggcagaac ctggtgaacg cggggattct tccgctgctc ttcgaggaca
      301 gggccgatct tgagaagatc gcgcaagggg accggctgga gctggtaaat ctgcactctt
      361 gcctcgaagg ggagtttgtc gaggtgcgga atgtgacgac gggagcttgt atcaggacgc
      421 gccacgggct ttcgcggaga cagcgggaga ttctgctcgc cggcggggcg ctcaacgccg
      481 cccgtgttgc gctgacgaaa gaaccgtaac ggtcgaaagg gggagcgcgc atgttcggac
      541 tggaggagcg ggcgctggtc tcgccgaggg agatggagga acgctcgcga ttgctggcgt
      601 cgaggggcct cacggttccg ggaaacgaat ctgtgatcct gggatttttc gaggaagagc
      661 gtcttgtcgc gaccggctcc ctggtgggga atattctgca ggggatcgcg gtcgatcggg
      721 aacttgaggg ggagggcgtc gccgcgacgg tgacctccgc tctgctgaaa aaagcgatgc
      781 agatggggcg caggcagctc ttcctgtact cgaaaccgga cgaggcgaag cgcttcgagg
      841 cgctcggctt cacgctgctg gcgactgcat cggtggagcg gacgggtctt ggagcttcgc
      901 tgctggagtg gggctccgaa ggaatagaca aatggagagc ggcgatagcc acgaaggcgg
      961 agggcggccc ggagggggcc ggcgtcgcgg tggtgaactg caacccgttt acgctggggc
     1021 actactgcct gctggagtat gccgccgcac gaacgccgcg gctctatgtc ctcgtcgtgg
     1081 aggaggaccg gtcgctcttc cccttcgaag tccgcttccg ccttgtcaag gagggagtgt
     1141 ccgagctgaa gaacgtcgta gcgcttcgcg gcgggccgta cgtcatctcg tcggcgacct
     1201 tcccgtcgta tttcacgcgg atcgaatccg gcgaggagga acggattacg gagctctacg
     1261 ctgcgctcga catcgaactc ttcggacggt acgtcgcttc ggcgctcaag gcgacggagc
     1321 gtttcgtggg gacggagccg tattgccccg tgacctccat ctacaataga atgatgaagg
     1381 agcgcctgcc gaagggcggc gagggatggc cgcccgtgcg cgtccatgag atgccgcgtt
     1441 tcgagatgga gggcatgccc gtcagcgcgt cgcgagtgcg ctccctgatc cgagaggacc
     1501 gtatggaaga cgtcaggccg cttgttcccg agtccacgtg gaagtggctc gtttcggatg
     1561 aagcgctccc tgtgatcgaa aaaatcagga acagcgagtc gagacattga atacggacgc
     1621 ttcgagtccg ccgaaaggag agggttgtat gaacggtgtg aagaacagcg tcgcggggtc
     1681 gctcgaatca agcgacgtgc tggtgacggt aggccccgcg gagggggcgg agaacacact
     1741 cgacgtggag agcatcgtct tcaaacagtt cggagcgcgt atccgctccg tcgccgagga
     1801 ggtcatgacc gcctcgggcg tcaggggcgc agatgtgcgg attcaggata ggggcgcgct
     1861 ggagtgcacg ctccgcgccc gtctggagac ggctctggag cgggcgctca aggcctgatg
     1921 acgtcccctc tcgacgccag ggaaacgcgg tggcggaagc ggattgatct tgcctccgcg
     1981 ctcccgcccg gctgttcgct gctctccttc acgctcagga tgcccgcgcc gctgcgcctc
     2041 ggcggcgagt acgaccggac tgccggggag tggttcgacg agcttcggca gtttctggcg
     2101 aaggagggga tgagtgcgcc ctcatatgac ttttacgtct ccgccgacgg gccggagggg
     2161 ttctgcaccg tccggggcga ggggaaggac gtgaagcgcc gcgcggtcgc cttcgaggag
     2221 acccatccgt gcggcgagct cgtcgacgtc gacgttacgt gtccggacgg ctccgtgccg
     2281 gggagacggg agctggggct tcctcctcgg cgctgcgtcg tctgcggcgg cgaggccgtg
     2341 ccgtgcgtga ggggacgcgc gcataccgcg gaggagctgc gaagcgctgt ggagcgtatc
     2401 gcggaggggt ggcgggcgtg ccggagggaa aggcgcagcg gactgaatgc gcctgcttcc
     2461 gatgaacgaa gcgcccgggg tgtcggggaa gggcgaacat ttggaacctg caac
//
LOCUS       DAOSWW010000361         2505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_32452_length_2505_cov_3.843265, whole genome shotgun sequence.
ACCESSION   DAOSWW010000361 DAOSWW010000000
VERSION     DAOSWW010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32452_length_2505_cov_3.843265"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            496..771
                     /locus_tag="PK442_07800"
     CDS             496..771
                     /locus_tag="PK442_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter permease"
                     /protein_id="HOO87590.1"
                     /translation="MAVHMTRSNEIQGSAAGRLLEMLLPRPTSTFLLRCADGPDSILV
                     VDRSLPPSEGKKAVLSTEKGLRLATLGSAFDSSRVWGVVTWVIREPS"
     gene            747..2036
                     /locus_tag="PK442_07805"
     CDS             747..2036
                     /locus_tag="PK442_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOO87591.1"
                     /translation="MGHPGAFLKPCEELLALCDCNNFFVSCERLFRPDLEGRPVVVLS
                     SNDGVIISRSNEAKTLGIKMGEPYFRVRAFLERRGGAVFSSNFQLYEEISARVMEVLR
                     RSTPSIEVYSVDEAFLELPPAARERPVEWGRELRAAVLKETGIPVSVGVAPAKTAAKL
                     AAERGKKRPETGGVFALLPGEAWDDILRDVPVEDVWGIGRQWSDFLRRRGVSTALRLR
                     DTPDDWLEKYLGVRGLRTAWELRGIRCFPIEEYEKPQKSIHSSRSFSTPVRSRESLAE
                     AVTEFALTAGRRLRSQRSLAGCLSVSISTSRFRSPFYGAREEVVLETPTDSDIALIRA
                     ATGALSSIYKDGCDYEKAEVGLGRLSSADTVQLRLFGGRDDRGSALSRAADRLNEQAG
                     RRLLAPAALLGEKEWRPRKDRHSGVRLEDLNTLPKIS"
     gene            complement(2058..2414)
                     /locus_tag="PK442_07810"
     CDS             complement(2058..2414)
                     /locus_tag="PK442_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87592.1"
                     /translation="MKIRYKIAVGGFQLFVAALIFAAEKGFSFAGSVFLVVGVLMLTR
                     PYAVFDESRSALTFTALLGPHSTENEAFLEGGEIMLLRKGKKRKLPRAFAWFANAKDF
                     EEMRGKLRESPLSGAE"
BASE COUNT          470 a          800 c          758 g          477 t
ORIGIN      
        1 cgtaatccac ggaaacggaa ggttcccccg cgtttgcggg tcgggcggaa accagaaacg
       61 ctccgaacgc caggagcgca catacaacca cgatcatatt tttcatgaca tctctcctcg
      121 tgagtggaaa aaatctttcc ttccttcata tcggcacgcc tcaactcccc ggcgcataca
      181 caggacacga tgatgttccg gcagccgctt tctcatgtcc ggcggcaaca gatacatcgg
      241 agcgctgccg ctgcttttga aattatacac cgcaatgatc cgcccgcagc agtctttaca
      301 gcgtcatacc ctctcggaac gcacttccgt ctgtgaatac tctcaaccag cgtttcctcc
      361 gcgacaggct ctcaaataga tactttaaga aatctataca aggtattgac tttctatgtg
      421 tatattatta tactgacacg caaagactca ctgtaatcat tatacacaaa aacagtctga
      481 aggagagtgc attctatggc cgttcacatg acgcgaagca acgagattca gggcagcgcg
      541 gccggtcgcc tgctggagat gctgcttccc cggccgacct ccacatttct tctgcggtgc
      601 gccgacgggc ccgactccat cctcgtggtg gaccgctcgc tccccccttc ggaggggaag
      661 aaggccgtcc tctccacgga gaaggggctc aggctcgcca cattgggctc cgcgttcgat
      721 tcttcaaggg tgtggggggt ggtgacatgg gtcatccggg agccttcctg aagccttgcg
      781 aggagctgct cgccctctgc gactgcaata acttcttcgt ctcgtgcgaa cggctcttcc
      841 gccccgacct tgaggggcgt cccgtggtgg tgctctccag caacgacggg gtgatcatct
      901 cccgctccaa cgaggcgaag actctcggta tcaaaatggg ggagccctac tttcgggtgc
      961 gcgcctttct ggagcggcgc gggggcgcgg tgttctccag caactttcag ctctacgagg
     1021 agatatcggc cagggtcatg gaggtcctcc ggcggagcac gccgtccatc gaggtctatt
     1081 ccgtggacga ggcgtttctg gagcttccgc ccgcagcgag ggaacgcccg gtcgaatggg
     1141 ggcgcgaact ccgggccgcc gtgctgaaag agacggggat tcccgtctcg gtgggcgtcg
     1201 ctcccgcgaa gacggcggcg aagctggccg ccgaacgggg caagaagcgg ccggagacgg
     1261 gcggggtctt cgcccttctg ccgggagagg catgggacga cattctccgg gatgttcccg
     1321 tggaggacgt gtgggggatc gggcggcagt ggagcgactt cctccgcaga cggggcgtgt
     1381 ccacggccct tcgcctgcgg gacacgcccg acgactggct ggagaagtat ctcggcgtcc
     1441 gggggttgcg gacggcgtgg gagctgcggg ggatacgctg ttttcccata gaagaatatg
     1501 aaaaacccca gaagtccata cactcctccc gctccttctc cactcccgtc cggagccggg
     1561 aatctctcgc cgaggcggtg acggagttcg cccttactgc ggggcggcgg ctccgctccc
     1621 agcgctccct ggcgggatgc ctctccgtga gcatctccac cagccgcttc cggagcccct
     1681 tctacggcgc cagggaggag gtcgtcctgg aaacgcccac cgacagcgac atcgccctca
     1741 tcagggccgc gacgggagcg ctttcgtcca tttacaagga cgggtgcgac tacgaaaagg
     1801 cggaggtggg gctgggacgc ctctcctccg ccgacacggt gcagcttcgc ctcttcggcg
     1861 gaagagacga caggggatcg gccctctccc gggcggcgga ccggctgaac gagcaggccg
     1921 gccgcaggct gctggcgcct gcggcgctgc tgggggaaaa ggagtggcgc ccccgcaagg
     1981 acaggcactc gggagtgcgc ctcgaagacc tgaacacgct cccgaagatt tcgtgagagg
     2041 cgccgttcgc ccgttcctca ctccgcaccg gaaaggggcg attcgcgcag ctttccgcgc
     2101 atctcctcga agtccttggc gttggcgaac caggcgaacg cgcgcggcag tttgcgcttc
     2161 ttccctttgc gaagcagcat gatctcgccg ccctccaaaa acgcctcgtt ctccgtcgag
     2221 tgcggcccca gaagcgccgt gaacgtcagc gcgctccggc tttcgtcgaa cacggcgtag
     2281 ggccttgtaa gcatcaacac ccccacgacg agaaataccg atcctgcgaa cgaaaagcct
     2341 ttttccgccg cgaagatcag cgcagcgaca aaaagctgaa agcctcctac ggcgatcttg
     2401 tagcgaattt tcatatcaac gcctcctttc cgtgccttcc gcacagtgcg gagagaggga
     2461 aggcgcaggc ctcccgcccg ttccttccct ctctcttcat ccgct
//
LOCUS       DAOSWW010000362         2498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_32606_length_2498_cov_3.260336, whole genome shotgun sequence.
ACCESSION   DAOSWW010000362 DAOSWW010000000
VERSION     DAOSWW010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32606_length_2498_cov_3.260336"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PK442_07815"
     CDS             complement(<1..783)
                     /locus_tag="PK442_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87593.1"
                     /translation="MRNALSVQNLVKRFGTFTAVNNVSFDVEDGKFFSILGPSGCGKT
                     TLLRMIAGFYQQDEGTILFNGQDMEGVAPNKRPVNLVFQNLALFPMMNVHENVAFGLR
                     RRGEKGAAVERKVKDMLERVGLPESGKKEVSQLSGGQKQRIAIARCLVLEPTVLLLDE
                     PLGALDAKLREHMKVELKTLQAKVGTTFDYITHDQSEAMVMSDRVAVMNNGAFEQIGT
                     PQELYHAPSSSFVAQFVGANNKLSVSLRRGEGGELLARYANGL"
     gene            complement(867..1937)
                     /locus_tag="PK442_07820"
     CDS             complement(867..1937)
                     /locus_tag="PK442_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO87594.1"
                     /translation="MKRFLIVLCVFALMFSVLAPASSEASVLRVITWSGYAPQELLDK
                     FTAETGIKVEVTLSNNEEMISKLRATRGGGFDLAQPSQDRISSVVEQYKIYQPIDFSK
                     IDEAQIDPSLLEAVKKNTLVDGKSYAVPHVYGTEGLVVNKAKAPNIKDFKDLLDPQYA
                     GRVSYRLKRPTLIGMGFSLGYDPFSLYTKTEEYQKFLDEMEKVLIAGKPAVKTYWDNG
                     DQLLQLLRSGEVWAASAWEQAGWKLHAENPDIDYIAPTSGALAWVDTFAIPARSENLE
                     AAYAYINFMLRPENAAFFTNRETYGTASKDAVKHLDPEIKANFERGLPPEVLAKINWY
                     PTVPAGVEEMEGKTLDKVKAAR"
     gene            2230..>2498
                     /locus_tag="PK442_07825"
     CDS             2230..>2498
                     /locus_tag="PK442_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO87595.1"
                     /translation="MEKKTILVLTGSPRKGGNSDMMAEAFIEGATSVGHEVLRFDAGR
                     NTVNGCRACGACWAKGSACVFEDGFTELAQLLVKADVLVFSTPLY"
BASE COUNT          434 a          754 c          817 g          493 t
ORIGIN      
        1 aagcccgttc gcgtaccgtg cgagcagctc gcccccttcg cccctgcgga gcgatactga
       61 gagcttgttg ttggcgccga cgaactgcgc cacgaacgag gaagatggcg cgtggtagag
      121 ctcctgcggc gtgccgatct gctcgaaggc gccgttgttc atcacggcga ccctgtcgct
      181 catcaccatc gcttcggact ggtcgtgcgt gatgtagtcg aacgtcgtcc ccaccttggc
      241 ctggagcgtc ttcagctcca ccttcatgtg ctcgcggagc ttcgcgtcga gcgcgccgag
      301 cggttcgtcg agcagcagca ccgtcggctc cagcaccagg cagcgcgcga tggcgatgcg
      361 ctgcttctgt ccgcccgaga gctgactgac ttccttcttc ccggactccg gcaggccgac
      421 ccgttcgagc atgtccttca ccttccgctc cacggcggcc cccttctcgc cgcgacgccg
      481 caggccgaac gcgacgtttt cgtggacgtt catcataggg aagagggcga gattctgaaa
      541 gacgaggttc acggggcgct tgttcggggc cacgccctcc atgtcctgcc cgttgaagag
      601 gatggttccc tcgtcctgct ggtagaaccc ggcgatcatc cggagcaggg tggtcttgcc
      661 gcaccccgag gggccgagga tcgagaagaa cttgccgtcc tccacgtcga aggagacgtt
      721 gttcaccgcg gtaaaagtgc cgaatcgttt cacgaggttc tgtactgaaa gagcgttgcg
      781 catacggcgg tcacctctga atgtgcagca ggcggcggcc tcttcgcgaa gaccgccgcc
      841 tgccggcagg atagatgacg gctgcgttat ctggccgcct ttactttatc gagcgtcttt
      901 ccttccatct cctccacgcc cgccggaacg gtggggtacc agttgatctt cgcgagcacc
      961 tcgggcggca agccgcgctc gaagttcgcc ttgatctccg ggtcgagatg cttcacggcg
     1021 tccttggagg ctgtgccgta ggtctcgcgg ttggtgaaga aggccgcgtt ttcggggcgg
     1081 agcatgaagt tgatgtaggc gtaggccgct tccagattct cggagcgggc cggaatggcg
     1141 aaggtgtcga cccaggcgag cgcgccgctc gtgggggcga tgtagtcgat gtcggggttc
     1201 tcggcgtgga gcttccatcc ggcctgctcc cacgcggacg cagcccagac ttcgccggag
     1261 cggagcagct ggagcagctg gtcgccgtta tcccagtacg tcttcacggc gggcttgccg
     1321 gcgatgagga ccttctccat ctcgtcgagg aacttctgat actcttcggt cttggtatac
     1381 agggagaacg ggtcgtaccc cagcgagaag cccatgccga tcagggtggg gcgcttcagc
     1441 cggtaggaga cgcgcccggc gtactgcgga tcgagcaggt ccttgaagtc cttgatgttc
     1501 ggggccttcg ccttgttgac cacgagcccc tccgtgccgt agacgtgcgg cacggcgtag
     1561 gacttgccgt cgaccagcgt gttcttcttc accgcttcca gcagcgacgg gtcgatctgc
     1621 gcctcgtcga tcttcgagaa gtcgatgggc tggtagatct tgtactgctc cacgacggag
     1681 gagatgcggt cctgcgaggg ctgggccaag tcgaagccgc cgccgcgggt cgcgcgcagc
     1741 ttgctgatca tctcttcgtt gttgctcagc gttacctcga ccttgattcc cgtctcggcg
     1801 gtgaacttgt cgagcagctc ctgcggggcg taccccgacc acgtgatcac ccgaagcacg
     1861 gacgcctcgg acgacgcggg ggcaagtacg gagaacatga gcgcgaaaac gcacagcacg
     1921 atcaaaaacc tcttcattca tatccctcct gttgtagtgt ggtgaactgt ggaactcccg
     1981 aaaggcctgc gggttccctc ccctgcgcgg gggtctgtaa cgaacccgta catacgattc
     2041 tagtagttca ggggggcgaa gtcaacagaa atttgtttca aatcggttac aacacgacgc
     2101 cggaaagagt ggacggcgat cccgaaatgc tcgcggcaaa gagtataata gcggtcgggg
     2161 ttcgccccgg aacgaccgga aagcgtcgcc gggaagagcg ttcgatacag ccgaagggga
     2221 gtgcatactg tggaaaagaa gacgattctc gtcctgaccg gaagtccgcg caagggcggc
     2281 aacagcgata tgatggcgga ggccttcatc gagggcgcaa cgagcgtcgg gcatgaggtg
     2341 ctcaggttcg acgcggggcg gaacacggtg aacggctgcc gcgcgtgcgg cgcctgctgg
     2401 gcgaagggga gcgcctgcgt cttcgaggac ggcttcacgg agctcgcgca gctgctggta
     2461 aaggcggatg tgctcgtctt ctccacgccg ctctactg
//
LOCUS       DAOSWW010000363         2496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_32650_length_2496_cov_3.433019, whole genome shotgun sequence.
ACCESSION   DAOSWW010000363 DAOSWW010000000
VERSION     DAOSWW010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32650_length_2496_cov_3.433019"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK442_07830"
     CDS             complement(<1..453)
                     /locus_tag="PK442_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOO87596.1"
                     /translation="MEQQETFEKRMRPAWRSFLTWWILSVAVAALAVFLSVRYTAPWW
                     TVAVALIPVVVIMLKRCSTEFIVKHDEVTLEKGLFAKNSVEVGMPQIRTIEVRQTVFQ
                     RMLGIGDIMIASSGTDTYEIIVSGIEEPKELRDVIQGFQRRAAKTLNDI"
     gene            complement(764..1954)
                     /locus_tag="PK442_07835"
     CDS             complement(764..1954)
                     /locus_tag="PK442_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO87597.1"
                     /translation="MSSKTFNLLRPESSGFKLSLLSTGHFFNDLYASFLPTFIPTIIS
                     RLGITMAQAGFFSTFLGIIHIIFQPIIGYLSDRSANPWLVIWGPIMTCLGATMIPLSP
                     SYGAALFFVGLWALGSAMFHPQGHGGVGHVVPREKLTVSLALFAVAGTAGVTVSPLFA
                     VALVNTVGFRLMPVVAIIPVLLLGLFTWRTMPSISHESGDAMTPQKGLLATLKSVFVV
                     IYPVWALSTVRDAASQGVRMFLPIRIASEGGEIGMVGTVLFLIMLSSTISMVLIGKIA
                     DRWGKKRVLLGVMALTPLSLLAGVYTENWVSIFCFLIGTSAINATLPVTAAIAQEMVP
                     NSRGMASSIVMGLSWGFGNMLMAPLGKIGDLFGVQTTLLVVAFLPLIALPLLYTKQFR
                     EMAE"
     gene            complement(2148..>2496)
                     /locus_tag="PK442_07840"
     CDS             complement(2148..>2496)
                     /locus_tag="PK442_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87598.1"
                     /translation="EAAMELSEAIMGKVADDNIESAIFLMRPFFRLPDAEFKILLEQS
                     KLQIPIIKSRFGTPLEHELIEQRFIGKSLMRIVHLQKYKYHAMRWVFVFYNPDGSWYI
                     NSFNFDDKIKELF"
BASE COUNT          706 a          680 c          598 g          512 t
ORIGIN      
        1 gatgtcattc agggttttcg cggctctcct ctgaaaaccc tgaatgacat cgcgaagctc
       61 tttgggctcc tctatgccgg aaacaataat ttcatacgtg tccgttcctg aggaagcgat
      121 catgatatcg cctataccaa gcattctctg aaaaaccgtc tgccggactt caatggtacg
      181 gatctgcggc ataccgactt caacagaatt ctttgcaaac agccccttct ccagagttac
      241 ctcgtcatgc ttcacgatga actccgtcga acagcgcttc agcatgatga caacaacagg
      301 aatcaatgcc actgcgactg tccaccacgg cgctgtgtaa cgcacactga gaaaaacagc
      361 gagcgcggcc acagcaacac tcagtatcca ccacgtcaaa aaacttctcc atgctggacg
      421 cattctcttc tcgaacgtct cttgctgctc cataaataaa cccctcccgt cacaaatttt
      481 atagcttctt cgaaatcaac cgataaacac gcgccaacgg ggtaccgcca ttcagagcat
      541 ggatgcttat tatctcatac gtgaaggctc gttttcctga aacgactctg cgctccaaaa
      601 aaacatttcc gacgcgctgt aaggacgacg catcggaaac attttacacc accttggcat
      661 tccatggaag aagaaagcag caaaagtacg caaaaaaacg aaacgagcaa gaacaaactt
      721 cagatgcgag cgtttcccct ccacttacct caacagaaaa atactattcc gccatttccc
      781 ggaattgttt tgtatagagc aacggcaacg caatgagcgg aagaaaggcc accacgagaa
      841 gcgtcgtctg cacgccgaag aggtcgccga tcttccccag cggcgccatc agcatgttgc
      901 cgaatcccca tgagagcccc atgacaatgg agctcgccat accgcgggag ttggggacca
      961 tctcctgcgc tatggcggcc gtgacgggga gcgtggcatt gattgcggaa gttcctataa
     1021 gaaagcaaaa aatactcacc cagttctcgg tgtacacgcc tgcaaggaga gagagcgggg
     1081 tgagcgccat cacacccagc agcactctct tcttccccca cctgtcggcg atcttgccga
     1141 taaggaccat gcttatcgta ctcgagagca tgatgaggaa aaggacagtg cccaccatgc
     1201 cgatctcccc tccttcggag gcgatacgaa tgggcagaaa catacggacg ccctggctcg
     1261 ccgcgtcgcg gacagtagag agagcccata cagggtagat gacgacgaag acggacttca
     1321 gcgtcgccag cagccccttc tgcggcgtca tagcgtcgcc gctctcatgc gaaatcgacg
     1381 gcattgtcct ccaggtgaac aagccgagta gaaggacagg gattattgcc accacgggca
     1441 tcaggcgaaa acctactgtg tttaccaagg ccaccgcgaa gagcgggctg accgtaacac
     1501 ccgccgtccc ggcaactgcg aagagagcca aagagacagt aagtttttcc cggggaacga
     1561 cgtgccctac cccgccgtgc ccctgcgggt ggaacatagc gcttccaagc gcccagagtc
     1621 cgacgaaaaa gagcgccgcc ccgtaggacg gagaaagcgg gatcatcgtc gccccgaggc
     1681 acgtcatgat cggcccccag ataacgagcc aggggttcgc cgagcggtcg gagagatacc
     1741 ctatgatcgg ctgaaagata atatggataa ttccgaggaa agtgctgaaa aagcccgcct
     1801 gcgccatcgt aatccccagc ctggagatga tcgtcgggat gaacgtaggc aaaaaagacg
     1861 cgtagaggtc attgaaaaag tgccccgtcg agagcagcga aagcttgaaa cccgaactct
     1921 cggggcgcaa cagattaaaa gtttttgacg acataaaaca aactcactcc ttgtgattat
     1981 actttaagct aaatactatc atacaccatg cagaatgtaa tagggggagg aaggtgaaaa
     2041 tacataatgc aaaaacggcc tttgcggaag gccgttttta cagcaacgga aagacgatcg
     2101 aaagtggaat tttaatcagg ctttcaagac ctttcagcga aaaaacctca aaaaagttct
     2161 ttgatcttat cgtcaaagtt aaaggaattg atataccatg atccatcggg attatagaaa
     2221 acaaacaccc agcgcatcgc gtggtacttg tatttctgaa ggtggactat tctcatcaac
     2281 gatttcccta tgaatcgctg ttcaatgagt tcatgttcca atggagtccc gaaacggctt
     2341 ttgataatag gaatctgcaa tttactctgt tccagaagta ttttgaactc tgcgtcgggg
     2401 aggcggaaaa acggccgcat cagaaaaata gcactctcga tattatcatc ggcaaccttt
     2461 cccataattg cttcggataa ctccatggcg gcctcc
//
LOCUS       DAOSWW010000364         2492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_32747_length_2492_cov_2.878539, whole genome shotgun sequence.
ACCESSION   DAOSWW010000364 DAOSWW010000000
VERSION     DAOSWW010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32747_length_2492_cov_2.878539"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1859
                     /locus_tag="PK442_07845"
     CDS             <1..1859
                     /locus_tag="PK442_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO87599.1"
                     /translation="IGRYNPKGPTIQRCLSGGLWDAGAGISVGGKHVANWLIGQVRDE
                     SQNEEAIRAYAREIGADEEEAAAAFREVPAMSRDRFQKIARMLFTLVQQLSELAYQNV
                     RQTLFIAEKNRAETIRKVQHELAEVMATAGSEIEVFDMARRELAGLMETKNFLIVDYD
                     ETTRMLSTRMHLGVDEKKLPVRWSADKALTGIMMAARKAMHFSRTEIIRLVDEGLIEF
                     VGDRAETWVGVPLQNEKKVFGAMILQSYTDPYAYDDWCVEILQSVGNQLSVYLEKKRA
                     EGALRESEEMFRAFFEQNSVGFAMINTDGSWLRFNDKLCEMTGYSREEMQEMNWRDFT
                     PPEEHQRERPLLNDLISGKIDSYTIEKRCIRRDGSTIDIFVLTKPLRRPDGEFRYFAS
                     IIQDISERKKNERELARHQEAIIGSMAILAERRDHGTGEHIQRTKFYVQRLLELSGGE
                     RFYPLEHLPLLAQAAILHDIGKVGVPDSLLLKPGKLTPEEFEVVKQHTVIGGDVLAAA
                     ARILGEAPFISYARQIIEYHHEKWDGTGYPHGLKGEEIPFIARIMAIADVYDALVSER
                     PYKGPIDHEKAVAIIVEGAGTHFDPVLVEAFLACADEFKSISQSGDTPVPGR"
     gene            1944..>2492
                     /gene="zupT"
                     /locus_tag="PK442_07850"
     CDS             1944..>2492
                     /gene="zupT"
                     /locus_tag="PK442_07850"
                     /inference="COORDINATES: protein motif:HMM:NF003243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOO87600.1"
                     /translation="MEIENVLFAFGLTLFAGLSTGIGSALAFFTKRTNTRFLAFSLGL
                     SAGVMIYVSMVEIFFKAQKALQADLGERMGAWVTVVSFFLGILGVMLIDKFVPSHENP
                     HEMHRIEEIRGRAGELGQEHSLMRMGILTALAIAIHNFPEGIATFMAAVQDPRLGVPI
                     AVAIAIHNIPEGIAVSVPIYYAT"
BASE COUNT          541 a          740 c          751 g          460 t
ORIGIN      
        1 tcatcgggcg gtacaacccc aagggaccga cgattcagcg ctgcctcagc ggagggctct
       61 gggacgcggg cgctggtatc tccgtcgggg ggaagcacgt cgccaactgg ttgatcggac
      121 aggtccgcga cgagagccag aacgaggagg cgatacgcgc ctacgcccgc gaaatcggcg
      181 cggacgagga agaagcggct gctgctttcc gcgaggttcc cgccatgtcc agggaccgat
      241 tccagaaaat tgcacgaatg ctcttcacgc ttgtacaaca gctctccgaa ctcgcctacc
      301 aaaacgtccg gcagacgctc ttcatcgcgg agaaaaaccg cgcggaaacc atcaggaagg
      361 tacagcatga acttgccgaa gtcatggcga cggccggctc ggagatcgag gtgttcgata
      421 tggctcgaag agagctcgcc gggctgatgg agacgaagaa ttttttgatc gttgattacg
      481 acgaaacgac aagaatgctc tcgacgcgta tgcatctggg cgtggatgaa aaaaagctgc
      541 cggtacgatg gtccgcggac aaagcgctga cgggcattat gatggccgcc agaaaggcga
      601 tgcacttttc ccgaactgag attatacggc tcgtcgacga ggggctcatt gaattcgtcg
      661 gtgatcgggc cgaaacatgg gtcggcgtgc cgctgcagaa cgagaaaaaa gtcttcggcg
      721 ccatgatcct tcagagttac acagacccgt atgcgtacga cgactggtgc gtcgagattc
      781 tgcaatcggt agggaaccag ctcagcgtct acctggagaa gaaacgcgcc gagggagcgc
      841 tccgcgagag cgaggagatg ttcagggcat tcttcgagca gaacagcgtc ggctttgcca
      901 tgatcaacac ggacggttcg tggctgcgct tcaacgacaa gctgtgcgag atgacgggct
      961 actcccggga ggaaatgcaa gagatgaatt ggcgcgattt cactccgccc gaggaacatc
     1021 agcgcgaacg ccctttgctg aacgatctga tctccggaaa gatcgattcg tacacgatcg
     1081 agaaacgctg tatccgtcgg gacggttcga cgatcgacat cttcgtgctt acaaaacctc
     1141 tgcggcgccc cgacggggaa ttccggtact tcgcctccat cattcaggac atctcggagc
     1201 gcaaaaaaaa cgaacgagag ctggcacgcc accaggaggc gatcatcggc agcatggcca
     1261 tcctggcgga gcgccgcgac cacggcacag gggagcacat tcagcgcacg aaattttacg
     1321 ttcagcggct gctcgaactt tcagggggcg agcgcttcta cccccttgag caccttccgc
     1381 ttctggcgca ggcggccatt ctgcacgaca tcggcaaggt cggcgtcccc gacagcctgc
     1441 tgctgaagcc cggcaagctg acgcccgagg agttcgaggt cgtcaaacag cataccgtga
     1501 tcggcggcga cgtgcttgcc gccgcggcaa ggatactcgg cgaggcgccg tttatcagct
     1561 atgcgcgcca gatcatcgag taccaccacg agaagtggga cggcacgggg tatccgcacg
     1621 ggctgaaagg agaggagatc ccctttatcg cgaggatcat ggcgatcgcc gacgtctacg
     1681 acgcgctcgt gagcgaacgc ccctacaagg gccccattga ccacgagaag gctgtggcga
     1741 tcatcgtcga gggggcggga acccatttcg accccgtcct tgtcgaggcc tttttggcct
     1801 gcgccgacga atttaaaagt atctcccaat ccggagatac ccccgtaccc ggccgctaaa
     1861 gaaacggcct cgcttgacgc cgccgtttcc tttcggtatt gtgtgtcggg gtacatgtga
     1921 catcatatca aggaggtcgc cacatggaaa ttgagaatgt actcttcgca ttcggtctca
     1981 cgctgttcgc cggcctctcg accggaatcg gcagcgcgct cgcattcttc acgaagcgca
     2041 ccaacactcg ctttctggcc ttttcgctcg gcctctccgc cggggtgatg atctacgtct
     2101 cgatggtgga gatcttcttc aaggcgcaga aggcgctcca ggccgatctg ggagagagga
     2161 tgggcgcctg ggtcaccgtc gtctccttct tcctcggcat tttgggagtg atgctgatcg
     2221 acaagttcgt cccctctcac gagaacccgc acgagatgca ccggatagag gagatacgcg
     2281 gccgtgccgg agagctgggg caggagcact cgctgatgcg catgggaatc ctcacggcgc
     2341 tcgccatcgc catccacaac ttccccgagg gaatcgccac cttcatggcc gcggtacagg
     2401 atccccgcct gggcgttccc atcgccgtcg ccatcgcaat ccacaacatc cccgagggga
     2461 tcgccgtctc ggtgcccatc tactacgcca cg
//
LOCUS       DAOSWW010000365         2485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_32903_length_2485_cov_3.489712, whole genome shotgun sequence.
ACCESSION   DAOSWW010000365 DAOSWW010000000
VERSION     DAOSWW010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32903_length_2485_cov_3.489712"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1324)
                     /locus_tag="PK442_07855"
     CDS             complement(113..1324)
                     /locus_tag="PK442_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOO87601.1"
                     /translation="MKFSERVLAMQSSPVRRLLPYAEEARAKGKKIYPLNIGQPDIKT
                     PESFLKAIKDFSQEVIAYATSQGNNDLRDAISRYYKEFNIPIEREDLIVTNGGSEALL
                     FAFMTMCDPGDEVLIPEPFYANYNAFAQSVNVKVVPITTKAEEGFHLPPKEQVEALVT
                     PRTRAILVANPGNPTGTIYTRDEMDMLADLALRHNLYIVADEVYREFVYDGLKYTSFG
                     HLPEIQDRVIIVDSVSKRYSACGARIGCIACKNKDFMAQVLKIAQGRLCVPTLEQVGA
                     TALYSTPKSYLDEVNKEYQSRRDTLYAALKKMPGVICSEPKGAFYVMVKLPVDDAEKF
                     IIWMLQNFDVDGETVMASAGEGFYATPGLGKQEFRLAYVLKNDELVKAMDILKLALEA
                     YPGRIEPVAAR"
     gene            1474..>2485
                     /locus_tag="PK442_07860"
     CDS             1474..>2485
                     /locus_tag="PK442_07860"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOO87602.1"
                     /translation="MKRMDILKSLATDNGKKMILLVMDGIGGLPGPEGKTELEAAATP
                     NMDALAKKSELGLLDMVDVGITPGSGPGHLSLFGYDPMEFSIGRGILEAMGVGALVGP
                     GDVCARGNFCTRSDDDVITDRRAGRIPTEKSALVVKKLAESIKEINGVRVTFYPGKEH
                     RFVVVFSGEGLSENVADADPQQEGSPMRWARPTVSDGERMADVANAFIKKVAEVLKGE
                     APANGCLLRGFSSAPDIPLLGDVYKIKPVAVATYPMYKGLAKLVGMNVVEAGETLDKL
                     FDSVGRLWSDYDYFYVHVKYTDSRGEDGDFEGKKKVVETVDALLPRLLALSPDVLVIT
                     GDH"
BASE COUNT          558 a          632 c          726 g          569 t
ORIGIN      
        1 acttcctctc ttcgttaaat gatctgcgga aaacataata acataccctg cggccgccgt
       61 ttttccggac cgcagggtat ggaaaggcgg agaagccttg tttacggaaa aatcagcggg
      121 cggctaccgg ctcgatacgt cccgggtagg cttcaagggc aagcttgaga atatccattg
      181 ccttgacaag ttcatcgttc ttcaggacgt acgcaaggcg gaactcctgt tttcccagtc
      241 ccggagtcgc gtagaatcct tcgccggcgg aagccatgac ggtttcgccg tcaacgtcga
      301 agttctgaag catccagatg atgaacttct ccgcgtcgtc gacggggagc ttcaccatga
      361 catagaaggc gcccttcggt tccgagcaga taactccggg catcttcttc agcgcggcgt
      421 agagcgtgtc gcggcgggac tggtattctt tgttcacttc atcaaggtac gattttggcg
      481 tactgtacag cgctgtagcg ccgacctgtt ccagcgtggg aacgcagagg cggccctgcg
      541 cgatcttgag aacctgcgcc atgaagtcct tgttcttgca cgcgatgcac ccgatgcggg
      601 cgccgcaggc gctgtagcgc ttggacacgg aatcgacgat gatgactctg tcctgtattt
      661 ccggcaggtg gccgaagctt gtgtatttca gtccgtcgta cacgaactcc cggtagactt
      721 cgtccgcgac tatgtagaga ttgtgtctga gcgcaaggtc cgcgagcata tccatctcgt
      781 cgcgggtata aatcgttccg gtcggatttc cagggttcgc cacaaggatt gcccgtgtcc
      841 gcggcgtaac gagcgcttcg acctgctcct tggggggaag atggaagcct tcttcggctt
      901 tggtcgtaat tgggacgact ttgacgttca cgctctgtgc aaacgcgtta taattggcgt
      961 agaatggttc agggatgagc acttcgtctc cggggtcgca catcgtcata aacgcaaaaa
     1021 gaagagcttc gctgccaccg ttggtgacaa taagatcttc tctctcgatt gggatgttaa
     1081 actctttgta gtagcggctt attgcatctc tcagatcgtt gttcccctgg gaggttgcgt
     1141 acgcaataac ctcctgcgaa aagtccttga tagccttgag gaacgattcg ggtgttttga
     1201 tgtccggctg cccgatgttg agaggataga tcttcttccc cttcgctcgc gcctcctccg
     1261 cgtacggcag tagtctgcgc accggagacg attgcatcgc caggactctt tcggaaaact
     1321 tcaccttgat gacccccttg aattgaaatt aagtggatgg accgctgaag aaaatgataa
     1381 cataaaatgc agaggttttt gcagaaaaaa acgagcactc gagacaaatc gatccattag
     1441 tgaaatgaaa acttttcgaa ggtggcgaga aatatgaaaa gaatggatat attgaagagc
     1501 ctggcgactg acaacggcaa gaagatgatt cttcttgtca tggacggtat aggaggtctt
     1561 cccggccccg aaggaaaaac ggaactcgaa gcagcggcga cgccgaatat ggacgccttg
     1621 gcaaaaaaga gcgaactcgg ccttctggac atggtcgatg tcggcataac tccgggaagc
     1681 gggcccgggc atctctccct cttcgggtac gacccgatgg agttctccat agggcgagga
     1741 attctcgaag ccatgggcgt tggcgcgctt gtaggtccgg gagacgtatg cgcgcggggg
     1801 aacttttgca cccgttccga cgacgatgtg atcaccgaca ggcgcgcggg gcgcatcccc
     1861 accgagaaga gtgcgcttgt cgtgaagaag cttgctgaat cgatcaagga gattaacgga
     1921 gtccgtgtaa ctttttaccc gggaaaggaa catcgtttcg tcgtcgtctt ctcgggagag
     1981 gggttgagcg agaacgtggc cgacgccgat cctcagcagg aaggcagtcc gatgcgctgg
     2041 gcgcggccga cggtttcaga cggcgaacgg atggcggacg ttgcaaacgc cttcataaag
     2101 aaagtcgcgg aggtgctgaa aggcgaagct ccggcgaacg gctgtcttct gcgcggattt
     2161 tcatccgctc ctgatatccc gcttttggga gacgtttata aaatcaagcc tgtggcagtt
     2221 gcaacctacc ctatgtacaa ggggctcgcc aaactggtcg ggatgaacgt cgtcgaagcg
     2281 ggcgagacgc ttgataagct tttcgacagc gtggggcgcc tttggagcga ctacgactac
     2341 ttctacgtcc acgtgaaata tacagatagt cgcggcgagg acggcgattt tgaaggaaaa
     2401 aagaaagttg tggaaacagt ggacgcgctc ctgccgcgtt tgcttgcgct ctcgcccgac
     2461 gttcttgtca ttaccggaga ccaca
//
LOCUS       DAOSWW010000366         2483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_32952_length_2483_cov_2.475700, whole genome shotgun sequence.
ACCESSION   DAOSWW010000366 DAOSWW010000000
VERSION     DAOSWW010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32952_length_2483_cov_2.475700"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..920
                     /locus_tag="PK442_07865"
     CDS             99..920
                     /locus_tag="PK442_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87603.1"
                     /translation="MKKSIFVLTVVCIFALLIVSGASAAEKSKEETLDAAYEHLVKVM
                     NPEEMKKLFALFDTMKDVGLSWNEDLLLPLRDIIACKTKEQLYVIWGMGSMNISYAML
                     FDKNPEGVWEGGREIDERIGFPRAELREESLAFAKSREGQASSSVDFIHAMLHRAKTD
                     QAVLNTLVGGFYGSTLELFYLFSSLGLASGVTEEFVQFVNAHLPQLTIAQEMLKAYSE
                     NGELAQLLDTGNRKAVIDSIVDIITKDMGKLTEDDLKQILSIVKAVRDPLTVPCN"
     gene            1012..>2483
                     /locus_tag="PK442_07870"
     CDS             1012..>2483
                     /locus_tag="PK442_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO87604.1"
                     /translation="MSMTEIVLNALLNLFALRAASAPSNLRGAVRKRVGFYLREHLGL
                     DAIGSYLELYDLTLDLQNEAPSELLLSRSAAVASGLRPQLPRDEQITILAMLLEAVPE
                     DAPSENEVLRAFAVERQFLVGYALFSGASFPWDGAGLDDRFLLPTDTPPQETRSSPFR
                     ILRRPQFKRAFAVFRAEEEGPWFIRASSRGALFLDGIPFDGGIRRLRQGSVVSNRQRN
                     RIHFGEIAAAFSALSATARPHILQGDHLDFRYDGAPDTGLHDFSFSVRGGELVAIMGG
                     SGSGKSTLLSLLTGKLRPRSGRLLLDGRDVAGDAMLRRGILGYVPQDDLLFEDLTVFE
                     NLYYSARLGLPDLPDAEVRRRCERMLDGLNQLDVAHIRVGSPLDKTISGGQRKRLNIA
                     LELIREPAVLLVDEPTSGLSSADSLHVVSLLKAQAAAGRLVVAVIHQPSSDVYKMFDR
                     LWMLDTGGRPIYDGNPLDALVHFRSASYRAGEQEYACPRCG"
BASE COUNT          515 a          784 c          717 g          467 t
ORIGIN      
        1 agtcggaaaa ggaaggggca taaagcggaa cggcgagcag cacgaatgca caaacgtaaa
       61 aaacaatcaa aaaaacaaaa taggaagggg aaacgtcaat gaaaaaaagt atttttgtac
      121 tgacagttgt gtgcatcttc gcactcctga ttgtctccgg agcttcggct gccgaaaaga
      181 gcaaggaaga gactctcgac gccgcgtacg agcatctggt caaggtaatg aatccggaag
      241 agatgaagaa gctcttcgcg ctcttcgaca ccatgaagga cgtcggtctt tcgtggaacg
      301 aggatcttct tcttccgctc cgggatatca tcgcctgtaa aaccaaagaa cagctctatg
      361 ttatctgggg tatgggttca atgaacatca gctacgcgat gctcttcgac aaaaatccgg
      421 aaggagtttg ggaaggcgga agggaaatcg acgaacgaat cggcttcccc agggcggaac
      481 tccgagaaga aagcctcgcg ttcgcaaaaa gccgcgaggg gcaggcgagc agttcggtcg
      541 acttcatcca cgccatgctg cacagggcga agaccgatca ggccgtgctc aacacccttg
      601 tgggaggctt ctacggcagc acgctggagc ttttctatct cttctccagc ctcggcctcg
      661 cttccggagt cacggaggag ttcgttcagt tcgtcaacgc ccaccttccg cagctgacca
      721 tcgcgcagga gatgctcaag gcttattcgg agaacggaga gttggcgcag ctgctcgaca
      781 cgggaaatcg aaaggccgtc atcgactcca tcgtggacat catcacgaag gatatgggga
      841 agctgacgga ggatgacctg aagcagattc tttctatcgt caaagccgtc cgcgatcctc
      901 tgaccgtccc ctgcaactga tccacgcgcc ggggcgtcct tcgcggacgc tccggcggaa
      961 ccgggcaacg ccgaaaacac tgctcttcac ttcggccaag gagactaagc catgtcgatg
     1021 accgaaatcg tcttgaacgc gctgctgaat ctcttcgcct tacgggccgc gtcggctccc
     1081 tcgaacctca ggggcgcggt ccggaaacgc gtgggtttct atctgcgcga acatctcggg
     1141 ctcgatgcca taggctccta tttggagctg tacgacctga cgctcgacct gcagaacgaa
     1201 gcgccttccg agctcttgct ttcacgaagc gccgcagtcg cctccggact gaggccccag
     1261 cttccccgcg acgaacagat cacgatcctc gcgatgctgc tggaagcggt tcctgaagac
     1321 gctccttccg aaaacgaggt gctccgcgca ttcgccgtgg agcgacaatt tctggtcggc
     1381 tacgcgctct tctccggcgc ttcattcccg tgggacgggg ccggactcga cgacaggttt
     1441 ctcctcccta cggacacccc cccgcaggag acgcgctcct ctccgttccg aatcctccgc
     1501 cgtccgcagt tcaaaagggc gttcgcggtc ttccgcgcgg aggaagaggg gccgtggttt
     1561 atcagggcct cttccagggg agcactgttt ctggacggca ttcccttcga cggcggtatc
     1621 cgccgcctga gacagggatc ggtcgtctcg aaccggcaac ggaaccgcat ccacttcggc
     1681 gagatcgccg ccgcgttctc cgccctctcc gcaacggccc gcccccacat cctccaggga
     1741 gatcatctgg acttccgcta cgacggcgcc cccgacaccg gccttcacga cttcagcttc
     1801 tccgtgcggg gaggcgagct tgtcgcgata atgggaggga gcggctccgg aaaatcgacg
     1861 ctgctctcgc tgctcacggg caagcttcgg cctcgctccg ggaggctgct gctcgacgga
     1921 cgcgacgtcg caggcgacgc gatgctccgg cgaggcattc tggggtatgt tccgcaggac
     1981 gatcttctct tcgaggatct caccgtcttc gaaaatctgt actacagcgc gcgtctcgga
     2041 ctgccggacc ttccggacgc agaggtccgg cgccgctgcg aacggatgct cgacggcctg
     2101 aaccagctcg acgtcgccca tatccgtgtc ggatctccct tggacaagac catcagcgga
     2161 gggcagagaa aacgcctcaa catcgctctg gaactcatca gggaacctgc ggtgctcctc
     2221 gtggacgagc ccaccagcgg gctctcctcc gcggactcgc tgcatgtcgt ctcgctgctc
     2281 aaggcccaag ccgccgccgg gagactggtc gtcgcggtga tccaccaacc atcgtcggac
     2341 gtctacaaga tgttcgacag actctggatg ctcgacaccg gagggcgtcc catctacgac
     2401 ggcaaccccc tggacgcgct cgtccatttc cgaagcgcct cctaccgcgc gggcgaacag
     2461 gagtacgcct gcccccgctg cgg
//
LOCUS       DAOSWW010000367         2470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_33245_length_2470_cov_3.086128, whole genome shotgun sequence.
ACCESSION   DAOSWW010000367 DAOSWW010000000
VERSION     DAOSWW010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33245_length_2470_cov_3.086128"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2262)
                     /locus_tag="PK442_07875"
     CDS             complement(<1..2262)
                     /locus_tag="PK442_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87605.1"
                     /translation="MIPDILKKTKGGASGSSLPNDELLVHQAESLILSGDLAAATEKI
                     GGAVSGESYAVRALLALLDDRRKDALALFEEGLKRLRKERGVRRIVFDSFSALYYPPL
                     LIASGDVKKAATYAELARQRSPYEPTYELLLCLAGFHSGKGSDKFFSVFSPARCKKEK
                     YKKGFLFHLALCLSWSSPEALIDPEYRSLFLNALDELRKSGNTFLLYCVELAEMLSLP
                     PEKQIPGRKLPLRDLIPRKEEWMVALGALTTLGKGTGGAKKKRERRILWEVDWDTEEN
                     GTIVSPEFIPVEQVMQSSGKWSKGRQVALKRLQRELVKEPWMTRQDLAAAGAVEEVGG
                     YDGWYYKRWFQFDGVKIFSALAGHPYLVRSGDGRPMEIVQEEPRLDLARTVGGYRVRL
                     SPHPAEGKYANVFLEEETPCRIRVVPFDEKHRRIAEILGKEGLFVPERGKEHILETLE
                     SLAETVPITSEIEGVEGSAVAVEPDSRIVVQLAPGEDGFFVSLAVRPLGTGTHLCRPG
                     EGGKTMMGRSDGQRIQTLRDLDAERRNRDEALVLCPALSEGEQTDENRWELDNPELCL
                     EFLLQLAGMGERVVVEWPKGGSMSVRSAVSPNAVKLSVATGRDWFALSGEIRVDEQTV
                     LGIKEACEMLAESKGRFLPLGNGEFLALTAELRKRLEEIGSFGDWRGKELRFSPLSAP
                     LFRKLEKETEGFGGDGEWAKQLALVEEAESIAPALPSTFKGVLRDYQTEGFQWLCRLA
                     HWGAGACLADDMGL"
     gene            complement(2278..>2470)
                     /locus_tag="PK442_07880"
     CDS             complement(2278..>2470)
                     /locus_tag="PK442_07880"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOO87606.1"
                     /translation="GHIASKTYDFYRTVSVTFGLPFIRTSVDHGTAFDIAWKGIANPV
                     SMKEAMLACFDIAPRYRPF"
BASE COUNT          446 a          801 c          745 g          478 t
ORIGIN      
        1 aagccccata tcgtccgcga gacatgcgcc tgcgccccag tgcgcgaggc ggcagagcca
       61 ctggaatcct tcggtctgat agtcgcgcag cacgcctttg aacgtcgagg gaagcgcggg
      121 agcgatcgac tcagcttctt cgacgagcgc gagctgtttg gcccactcgc cgtcgccgcc
      181 gaaaccctcc gtttctttct ccagcttccg gaaaagcggc gccgagaggg gggagaagcg
      241 cagctccttt ccccgccagt cgccgaacga gccgatctct tcaagccgct tccgcagctc
      301 cgcagtgagc gcgagaaact cgccgttccc gagggggagg aagcgtcctt tgctttcggc
      361 gagcatctcg cacgcctctt tgatgcccag aacggtctgt tcgtccacgc gaatctcccc
      421 ggagagcgcg aaccagtcgc gccccgtagc cacggagagc tttaccgcgt tcggggagac
      481 ggcggagcgg acgctcatcg agcctccctt gggccactcg acgacgacgc gctctcccat
      541 tcctgcaagc tggagcaaaa attcgaggca gagttcgggg ttgtcaagct cccatctgtt
      601 ctcgtccgtt tgttcccctt cgctcagagc agggcagagc accaacgcct cgtcgcgatt
      661 gcgccgctcg gcgtcgagat cccggagcgt ctggatgcgt tgcccgtcgc tccggcccat
      721 catcgtcttg ccgccctcgc ccggcctgca gaggtgcgtg cccgtcccga gagggcggac
      781 ggcgagcgag acgaaaaaac catcttcgcc gggggcgagc tggacgacga tgcgcgagtc
      841 cggctccacc gcgacggcgc tcccttccac gccttcgatc tcggaggtta tgggaacagt
      901 ctccgcaagc gactccaaag tctccagaat atgctccttc ccccgctccg ggacgaagag
      961 accttccttg ccgaggatct ccgcgatgcg gcggtgcttc tcgtcgaagg gaaccacacg
     1021 tatccggcag ggagtctcct cttccaggaa gacgttggcg tacttgccct cggcagggtg
     1081 cggagagaga cggacgcggt agcccccgac cgtcctcgcc aggtcgagtc tcggctcctc
     1141 ctgcacgatc tccatcggcc tgccgtcccc gctccggaca agatacgggt gccccgcgag
     1201 cgccgagaag attttcaccc cgtcgaactg gaaccatcgc ttgtagtacc agccgtcgta
     1261 gccgccgacc tcctcgaccg ctcccgccgc tgcgagatcc tggcgtgtca tccacggttc
     1321 cttgacgagc tccctctgaa gacgcttcaa tgcgacctgc cgtcccttgc tccactttcc
     1381 ggaggattgc atcacctgtt caacggggat gaactccggg gagacgatcg tgccattctc
     1441 ttcggtatcc cagtccactt cccagagaat gcgccgctcc cgcttttttt tcgctccgcc
     1501 ggttcccttc ccgagcgtcg tcaacgcccc gagcgccacc atccactcct ccttgcgggg
     1561 gatgaggtcg cgcaacggga gtttccttcc ggggatttgc ttctccggcg gaagcgagag
     1621 catctcggca agttcgacgc aatagagcag gaacgtattg ccggacttgc gcagttcgtc
     1681 gagggcgttc aagaagaggg aacggtactc cggatcgatc agcgcttccg gggaagacca
     1741 tgagagacag agcgcgagat ggaagagaaa tcccttttta tatttttcct ttttgcaacg
     1801 ggcgggagag aatacggaga agaatttgtc gctccccttg ccggagtgga agcctgcaag
     1861 gcagagcagc agttcgtagg ttggttcgta cggggaacgc tgtctggcaa gttcagcgta
     1921 cgtcgccgct ttcttgacgt ctccggaggc gatcagcagc ggcgggtagt aaagggcgga
     1981 aaaagagtcg aagacgattc gtctgacgcc gcgttccttc cgcagacgct tcaggccttc
     2041 ttcaaaaagc gccagggcgt ccttgcgccg gtcgtcgaga agagcgagca gcgcccggac
     2101 agcgtacgat tcgcccgaca cggcgccccc gatcttttcc gttgccgccg ccaagtctcc
     2161 ggagaggatg agcgattccg cttgatgtac gagaagctcg tcattgggaa gggaggagcc
     2221 ggaggcccct cccttcgtct tcttcagaat atccggtatc acgtcgcgcg ctcccggcta
     2281 gaacggacgg tagcgggggg cgatgtcgaa gcaggccagc atggcctcct tcatgctcac
     2341 cgggtttgcg atccccttcc atgcgatgtc gaaggcggtt ccgtgatcca ccgatgtccg
     2401 gatgaacggc agtccaaagg tgacgctcac cgtccggtag aagtcgtagg tcttcgaggc
     2461 gatatgcccc
//
LOCUS       DAOSWW010000368         2463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_33384_length_2463_cov_4.498754, whole genome shotgun sequence.
ACCESSION   DAOSWW010000368 DAOSWW010000000
VERSION     DAOSWW010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33384_length_2463_cov_4.498754"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PK442_07885"
     CDS             <1..860
                     /locus_tag="PK442_07885"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712825.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HOO87607.1"
                     /translation="TFSFMSVDWDGKIRMDCSSPYAMAGLVELKDRYDIAWGNDSDFD
                     RHGIVVPDRGLLPPNAYLAVAVEYLFTHRPGWGSDAAVGKTLVSSSLIDRVAHSVGRK
                     CVEVPVGFKWFVEGLLNGSMGFGGEESAGASFLRRDGTVWSTDKDGIILNLLAAEMLA
                     VTGEHPGKRYDAITARLGEPFYARIDAPASTERKKLLKTLSPEAVKASTLAGEPIVAR
                     LTAAPGNGAPIGGLKVTSENGWFAARPSGTEDVYKIYAESFLGAEHLTRIQSEAREIV
                     DGVFAAAGV"
     gene            complement(976..1188)
                     /locus_tag="PK442_07890"
     CDS             complement(976..1188)
                     /locus_tag="PK442_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87608.1"
                     /translation="MQNTRLNEASPVLSVLAVQGGRVTGKTAKERPKRDGRKRGFDDY
                     LRKAGYESRFDQELEDRLAESIDTLA"
     gene            complement(1728..>2463)
                     /locus_tag="PK442_07895"
     CDS             complement(1728..>2463)
                     /locus_tag="PK442_07895"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HOO87609.1"
                     /translation="EHALDFMELHMEQGRVLFEAKEPLGVVTAIAAPTRLKVTVLGRQ
                     DHSGATPMDLRKDALCAAADIILATEAAGRDEMRFATVATVGVANVVPNALNVVPGSV
                     ELRIDIRGIQRESIRRTVGKVQRAIEEIAASRGLSCATETISSMDPVTLAPSMIDSLA
                     EAASGTGVPFRKMASGAGHDAMKVAPVVPTGMLFIPCDEGISHNSAENASLNDIALGA
                     EVMLRSISLRSKHLSTADRSQPFSKL"
BASE COUNT          494 a          681 c          743 g          545 t
ORIGIN      
        1 cgacattttc cttcatgtcc gtggactggg acggcaagat ccgtatggac tgctcctctc
       61 cctacgcgat ggccggactg gtggagctga aggacaggta cgacatcgcc tggggaaacg
      121 actcggattt cgacaggcac ggcatcgttg ttcctgacag ggggctgctt ccgccgaacg
      181 cgtaccttgc cgtcgccgtg gagtatctct tcacgcaccg tccggggtgg ggttcggacg
      241 ccgccgtcgg gaagacgctc gtctccagtt cgctgatcga cagggttgcg cacagcgtcg
      301 gaaggaagtg cgtcgaggtt cctgtcgggt tcaagtggtt cgtcgaaggg ctgttgaacg
      361 gatcgatggg attcggcggc gaggagagcg cgggggcgtc cttccttcgc agggacggga
      421 cggtctggag taccgacaag gacggtatta tcctcaatct gcttgcggcg gagatgctcg
      481 cagtcaccgg agagcatccc gggaaacgtt acgacgccat cacggcccgc cttggagagc
      541 ctttttacgc gcgcatcgac gcgcctgcgt cgacggagcg gaagaagctg ctgaagacgc
      601 tctctcccga ggcggtgaaa gcgtcgacgc tcgccggaga gccgatcgtt gcccggttga
      661 cagcggcccc cggaaacggg gcgcccatcg gcggcctgaa agtgacgtcc gaaaacgggt
      721 ggttcgcggc tcggccgtcg gggaccgagg acgtgtacaa aatctatgcg gagagcttcc
      781 tgggggcgga gcatcttacc cgtatccaga gcgaagcgcg cgagatcgtg gacggagtgt
      841 tcgcggcggc cggagtctga tactcggatt tttcagcaag cggcctccgc gcacgatcgg
      901 acgcggaggc cgctgttctc tttctatgtt ccgaactctt ccggagacgt cttcctctgt
      961 tcgctgttcc gttgtttatg caagagtgtc gatgctttcg gcgagacgat cttccagctc
     1021 ctggtcaaag cgggactcat acccggcttt gcggagatag tcgtcaaaac cgcgttttct
     1081 tccgtctctt ttcgggcgct ccttcgccgt ctttccagtg acccgccctc cttggactgc
     1141 gagaactgaa aggactggag acgcctcgtt cagccgcgtg ttctgcatgg cgctcccccc
     1201 ttatagaaat gtatcggtag tgcgcgaaga gtttgttaat tccggatcag gagggggata
     1261 acacggaagg cggctctcat gagccgcctt cctacgcgtg cagaaaaaaa cgatgtggaa
     1321 aaaatctgta gagtagagag gaagaatgac tacgcttcct cgttgtttct gcttgcagag
     1381 cgttttttta ctacaagcat gacgacgccg ataacagcca tgatcccaag ccagatgaca
     1441 gtggcggtgt tcatagataa cacctccttt ttcaaaactt tggaagagct gtggctcaaa
     1501 tacacctcag aaaacccgcg cggaaaaacg aggctgaaaa aagtgaaaga gagtcggaga
     1561 actgcatcga tgtttcgttc ttaaactacc ataataggca gtttaattca agtcaaaatg
     1621 ataaatttca tcaatttact ttttttgctc ttcagatagt aatctttatt tatgtacttg
     1681 tagtgtattg ggaaaaattg cccgcgacgc tcggttttcg atatcctcta cagcttcgaa
     1741 aacggttgag agcgatcggc agtagaaaga tgtttgctcc tgagggagat gcttcggagc
     1801 ataacctctg cgccgagcgc aatatcgttg agcgaggcgt tttctgcgga gttgtggctg
     1861 atcccctcgt cgcaggggat aaagagcatg cccgtcggta cgacgggggc caccttcatt
     1921 gcgtcatgcc ccgcgccgct cgccatcttc cggaagggaa cgcctgtccc ggaagctgcc
     1981 tcggcgagcg aatcgatcat tgagggagcg agcgtcaccg gatccatgga ggagatcgtc
     2041 tccgtcgcac acgaaagacc tcgcgaagct gcaatctcct cgatggcgcg ctggactttc
     2101 ccgaccgttc tcctgatgct ctcgcgctgt attcccctga tgtcgatccg gagttccacg
     2161 ctcccgggga cgacgttgag cgcgttcggg acgacgttcg caacgcccac ggttgcgacg
     2221 gtagcgaagc gcatctcgtc acgccctgcc gcttcggtcg cgagaataat atcggcagcc
     2281 gcgcagagcg cgtccttacg caggtccatc ggggttgctc cggagtgatc ctggcgtccg
     2341 agaaccgtta ccttgaggcg cgtgggtgcg gcgatcgccg tgacgacgcc cagcggttct
     2401 tttgcctcga agagcacccg gccctgctcc atatgcagct ccatgaagtc gagcgcatgt
     2461 tcc
//
LOCUS       DAOSWW010000369         2461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_33434_length_2461_cov_3.187864, whole genome shotgun sequence.
ACCESSION   DAOSWW010000369 DAOSWW010000000
VERSION     DAOSWW010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33434_length_2461_cov_3.187864"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..121
                     /locus_tag="PK442_07900"
     CDS             2..121
                     /locus_tag="PK442_07900"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HOO87610.1"
                     /translation="MLYVGTLHAGVNLYNNGFAGGLVAAMLVPIFEAFGLKRD"
     gene            479..1240
                     /locus_tag="PK442_07905"
     CDS             479..1240
                     /locus_tag="PK442_07905"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF009093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HOO87611.1"
                     /translation="MLKEKVALVTGGASGIGLAIAESFAKDGAKVVVADINEAKLREE
                     AGRFGAEYFKADLSKREECKALVEYALQKFSKVDILVNVAGIQTVSPIENFPEDKWDF
                     MISLMLTAPFLLTRYVWPSMKAQGWGRIINLNSIHGLVASEFKSAYVSAKHGLAGLTK
                     TTALEGGAYGITVNSICPAYVRTPLVDNQIADQAKSHGISREEVIEKIMLQKASVKKL
                     IEPDTIADVAKFLCSDAGAHITGSTLTIDGGWTAA"
     gene            1309..>2461
                     /locus_tag="PK442_07910"
     CDS             1309..>2461
                     /locus_tag="PK442_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HOO87612.1"
                     /translation="MLGILIGLVLLMYLAYRGYSIIWVAPLTALVVAFTGDLPLIAAY
                     RDTYMSGFVGFTKSWFPVFMLGAIFGHLMDYTGAARSIAHWLTGIFGAKRAILGVIVG
                     CAVLTYGGISLFVVVFAMYPLALALFREANISRKLIPGCIALGSFTFTMTAIPGTPQI
                     QNLIPMQYFKTTPTAAPIMGITAAIFMFALGYAYMIWKERRFTARGEVFVEPAKKGEE
                     SESLYTDLPSPVISIVPLLTVILTLNLLKWDNVVALLAGIVLAMILNFKHYKGFTKTI
                     SGGATGSVLAIINTSAAVGFGSVVRAVPGFQTLTEFVLGIKGSPLISEAVAVNVLAGA
                     TGSASGGMGIALAALGDKYYQLALETGISPEAFHRVASLASGGLDTLPHN"
BASE COUNT          583 a          554 c          660 g          664 t
ORIGIN      
        1 tgtgctgtat gtaggaacgc ttcatgccgg agtcaattta tataataatg gttttgccgg
       61 agggcttgtt gcagcgatgc ttgtccccat ttttgaagct ttcgggttaa aaagagatta
      121 gaatgcatgt taagtgtcgt cgtttcatct tggagaaggg cgtttttttg cttaaatgaa
      181 gttaagaagt ttaataggaa tccattacat aaaaacatgc atcaagtcac tagaaagcat
      241 tttacatata gcctgtctat aatgtatgat ctcgacattc aaattatatc ttaatagccc
      301 tgaaatctct taagaatggc cgtgtcttaa gagccggtgt ttctatttgc ttgaaatccg
      361 caatagcatt tgtaaacgag gcttttttct ttgtattaag cgatcaatca tttgatcgtg
      421 cagttgttct tttgtttatt aaaaagacta atgaaacatc aaatagggag ggagttatat
      481 gttaaaggag aaggtggctt tggttacggg cggcgcaagc ggtatcgggt tggcgatcgc
      541 cgagtctttc gccaaggatg gtgcgaaagt cgtcgttgcg gacatcaacg aagcaaagct
      601 tagagaggag gcgggacgtt tcggagccga gtattttaag gcggatctca gtaaaagaga
      661 agagtgcaag gcgttggtgg agtatgctct gcagaaattt tcgaaggtcg atattctggt
      721 gaacgtggcg ggtattcaaa cggtatctcc catcgaaaat ttccccgaag acaaatggga
      781 tttcatgatc agcttgatgc tgaccgcgcc gtttcttctc accaggtacg tatggccctc
      841 catgaaggcg caaggctggg ggcggatcat caaccttaat tcaatccacg gtcttgtcgc
      901 gtcggaattc aaatccgcct atgtttcagc gaagcatgga cttgccggac taactaaaac
      961 aaccgcattg gaaggggggg catacggtat cacggtgaac tccatctgcc cggcgtacgt
     1021 cagaactccg ttggtcgata accagatagc ggatcaagcg aaaagccacg gtatttccag
     1081 agaagaggtt atagagaaga tcatgctcca gaaggcttcg gtcaaaaagc tgatcgagcc
     1141 ggatactatt gcggacgttg ccaagttcct gtgttcggac gcaggcgcgc atattacggg
     1201 gtcgacgttg acgatcgacg gtgggtggac ggcggcataa aacgaggttt tcaaacgaga
     1261 gaaaaagagc tttgccggct tttttataaa aagtgaggag ggagtttcat gcttggaatt
     1321 ttgataggtc ttgtgctgtt gatgtacttg gcgtacagag ggtattctat tatatgggta
     1381 gctcctttga cggcgttggt agtggcgttc acaggcgatc ttccgcttat cgccgcgtat
     1441 cgcgatacgt acatgagcgg cttcgtaggc tttacgaaat cctggtttcc tgtattcatg
     1501 ctgggggcga tattcgggca ccttatggac tataccgggg ccgccagatc cattgcccac
     1561 tggctgaccg gtatcttcgg cgctaaacgt gcgattctgg gcgtcatcgt cggatgcgcc
     1621 gtgcttacct atggcggcat cagccttttt gtcgtcgttt tcgcgatgta tccattggcc
     1681 ctcgctcttt tcagggaggc gaacatctcc agaaagctga tccccggatg tatcgctctc
     1741 ggttcgttca ccttcaccat gacggcgatc ccgggaactc ctcaaataca gaaccttatc
     1801 ccgatgcagt atttcaagac gactccgacg gcggccccca tcatgggaat tacggccgcc
     1861 attttcatgt ttgcgctcgg ttacgcgtac atgatatgga aggagcgacg atttacagcc
     1921 agaggcgagg tcttcgtcga acctgcgaaa aaaggagagg aatccgaaag cctgtacacc
     1981 gatctgccca gtcctgttat ctccattgtt cccctactta cagtcatttt aacgcttaat
     2041 ctgttgaaat gggataatgt cgttgccctg cttgccggaa ttgtgctcgc gatgattttg
     2101 aattttaaac attataaagg tttcacgaag actatcagcg gaggagcgac cggttccgtc
     2161 cttgcgatca tcaacacgag cgctgctgtc ggttttggat cggtggtcag agcggtaccc
     2221 ggttttcaga cgcttacgga gtttgtgctt gggatcaaag gatctccgtt gatttccgaa
     2281 gcggtcgccg tcaacgttct tgcgggagca acaggctccg cttccggcgg aatgggcatc
     2341 gcccttgccg ccctcggaga taagtactac caactggcgc tcgagacggg aatcagccct
     2401 gaagctttcc acagagtcgc ctcgcttgct tcgggcggtc tcgataccct tcctcacaac
     2461 g
//
LOCUS       DAOSWW010000370         2448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_33741_length_2448_cov_3.402424, whole genome shotgun sequence.
ACCESSION   DAOSWW010000370 DAOSWW010000000
VERSION     DAOSWW010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33741_length_2448_cov_3.402424"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PK442_07915"
     CDS             <1..694
                     /locus_tag="PK442_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOO87613.1"
                     /translation="KLPHIVIVVDELADLMFTAQKDVEDYICRLAQMARATGIHLVLA
                     TQRPSVNVITGLIKANIPARVAFTLPSQTDSRTIIDVSGAERLLGKGDMLFVSSKYPR
                     PLRLQAPFIEDGKSLEIIEYLGNIFGPPEYVDIEAQGAGESTNNGDTSFAEDPLLEEA
                     VDIVMETGIASASRLQRQLRVGFTRAARLVDTMEQIGIVGPPEGSKPREILVDDETAR
                     SLLTRVLSGEGE"
     gene            696..1406
                     /locus_tag="PK442_07920"
     CDS             696..1406
                     /locus_tag="PK442_07920"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOO87614.1"
                     /translation="MFSLSLPNGKATFSNAFQEPLLVVAGGRKPAASWLAEIFRSFTT
                     RWCADSGLVYCLEAGILPDRLVGDGDSTPPDEWRRAVEAGVSVTKHPVDKDLTDLQLA
                     LFTAGKELHGKPVLVTGCWGGRFDHLWANIFSALWAAEQDIRILVFADEKECLFLLEG
                     EEALEICVSGRENVVVSLLPLTPECAGVDLQNVRWELKDSLLLQKHPYTVSNRTEGNK
                     NISVRLQKGILGVYCGWL"
     gene            complement(1423..1617)
                     /gene="rpmB"
                     /locus_tag="PK442_07925"
     CDS             complement(1423..1617)
                     /gene="rpmB"
                     /locus_tag="PK442_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOO87615.1"
                     /translation="MAKVCECCGRGPTTGNAVSHSNRHTRRRWLVNLRSVKADVGGGE
                     SLRMRICTRCLRSGKVKRAV"
     gene            1729..1959
                     /gene="xseB"
                     /locus_tag="PK442_07930"
     CDS             1729..1959
                     /gene="xseB"
                     /locus_tag="PK442_07930"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOO87616.1"
                     /translation="MSFSAKMDELEGLVARIEKEALPLEDALALFEKGVGLVRECREY
                     LSEAKQRILLLTEDSSTVPFVATYNNDEESSR"
     gene            1956..>2448
                     /locus_tag="PK442_07935"
     CDS             1956..>2448
                     /locus_tag="PK442_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOO87617.1"
                     /translation="MTQNTRSFQALFSEAGEFVEARLQELAEMLPVPAPRRLRESMIY
                     SLTAGGKRLRPILCLKAAEVFGMSWDVSIPMALGFEMIHTASLIHDDLPAMDNDVLRR
                     GKATNHVLFGESLAILAGDALMAWAFEYPLSALSKSNVPAERICEAMRILANSLGPFG
                     ICGG"
BASE COUNT          540 a          603 c          707 g          598 t
ORIGIN      
        1 caagctgcct catatcgtta tcgtggtcga cgagctggcc gacctcatgt tcacagcgca
       61 gaaggatgtt gaagattata tttgccgcct cgcacagatg gccagggcga cgggtattca
      121 ccttgtcctt gccactcagc ggccgtccgt gaatgtcatc acggggctga tcaaagcgaa
      181 cattccggcg agagtagcat ttacccttcc gtcgcagacg gattcgcgta ctattatcga
      241 cgtatccggg gcggagcgtc tcctggggaa gggcgacatg cttttcgtca gttcaaagta
      301 tcctcgcccc ctccgtcttc aggctccctt catcgaagac ggcaagagcc ttgagatcat
      361 agagtacctg ggaaatatct tcggcccgcc tgagtacgtc gatatcgaag cgcagggcgc
      421 tggagagagc acgaacaacg gcgatacctc ttttgccgag gacccccttc ttgaagaggc
      481 cgtcgatatc gttatggaaa caggtatcgc atcggcaagc agattgcagc ggcagctccg
      541 cgtaggcttt acacgcgctg cgcgtctcgt ggataccatg gagcagatcg ggattgtcgg
      601 acctcctgaa gggtcaaagc ctcgtgaaat acttgttgac gacgaaacgg cgaggagcct
      661 cctcacacgg gtgctttcgg gagagggaga gtagggtgtt ctctctctct cttcccaatg
      721 ggaaggcaac cttctcaaac gctttccaag agcctcttct ggtcgttgca gggggaagaa
      781 aaccggctgc ttcctggtta gctgagattt ttcgctcgtt tacgacgcgc tggtgcgctg
      841 attccggcct tgtgtattgt cttgaagcgg gtattctgcc ggacagactt gtgggagacg
      901 gagacagtac tcctccggat gaatggaggc gtgctgtaga ggcgggagtt tccgtaacga
      961 aacaccctgt ggataaggat ttgacagatc ttcagctggc gctcttcacg gcgggaaaag
     1021 agctccacgg aaaaccggtt ctggtaaccg gttgctgggg agggcggttc gaccatctct
     1081 gggcgaatat attctccgcg ttgtgggcgg ccgaacagga cattcgaatt cttgtatttg
     1141 cggatgaaaa ggagtgtctg ttcctcttgg agggggaaga agctctcgaa atttgcgttt
     1201 ccgggcgtga gaatgtcgta gtgtcgctgt tgccgcttac tccggaatgc gccggtgttg
     1261 atctccaaaa tgttcgttgg gagttgaagg attcccttct actgcaaaaa cacccctata
     1321 cagtgagcaa cagaaccgag gggaacaaaa atatctcagt aaggttgcag aagggaatcc
     1381 ttggtgtata ctgcggatgg ctctgaaaag caaaagaggg ctttacaccg cccgtttcac
     1441 tttgccggaa cggagacagc gtgtgcagat tctcatgcga agagattcgc cgccgccaac
     1501 gtcagccttg acgctccgaa gattgacaag ccagcgcctg cgtgtatgcc ggttagaatg
     1561 acttacggca tttccggtgg taggcccgcg accgcaacat tcacatactt ttgccattga
     1621 tttcgccccc ttgctgcgat tggtttcgat aaaacagaag aattttagca cagaggggtg
     1681 catggagcaa attttttttg tagaattcct cggggaggat tggacaaaat gagttttagc
     1741 gcgaaaatgg atgaactaga ggggttggta gcccgcatag agaaggaagc cctgcctttg
     1801 gaagatgccc tcgccctctt tgaaaagggg gttgggttgg tacgggagtg ccgggaatat
     1861 ctttcggaag caaaacagag aattcttctc cttacggagg atagcagtac cgtacctttt
     1921 gttgcgacct acaataacga tgaggagtca agccgatgac ccagaataca cgttcttttc
     1981 aagcgctttt ttcggaagcc ggggagtttg tcgaggcgcg cttgcaagaa cttgccgaga
     2041 tgcttcccgt ccctgccccc cgcaggctca gggaatcgat gatttattca ctgactgccg
     2101 gtgggaaacg tcttcgtccc attctctgcc tgaaggccgc ggaggttttc ggtatgtcgt
     2161 gggacgtgtc catacctatg gcacttggct ttgagatgat tcatacggcc tctctcattc
     2221 atgacgatct ccccgctatg gataatgatg ttttacggag agggaaggca acgaatcatg
     2281 tcctcttcgg cgaatccctc gccatactcg ccggagatgc gttgatggcc tgggcgtttg
     2341 aataccctct ctctgcgctt tcgaagtcga atgttcccgc tgagagaatc tgtgaggcaa
     2401 tgcgcatatt ggcaaactca ttggggccgt tcggtatttg cggcggac
//
LOCUS       DAOSWW010000371         2443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_33845_length_2443_cov_3.291457, whole genome shotgun sequence.
ACCESSION   DAOSWW010000371 DAOSWW010000000
VERSION     DAOSWW010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33845_length_2443_cov_3.291457"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1224
                     /locus_tag="PK442_07940"
     CDS             247..1224
                     /locus_tag="PK442_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO87618.1"
                     /translation="MKKWILVLTALALVFAALPAAADTVVLRLGETHVADYPTTKGNY
                     EFARLVEERTGGRIKIEVYHSSQLGQEKAVIEGVQFGAIDFTRVSISPLSAFAPAFDA
                     LQMPYLYRGEEHMWKVLNGSIGEEFLASLEPANFVGLAWYDSGARNFYNSKKEIKSVA
                     DLKGMKIRVQESQLMMGLVSSLGAVPTPMPFGEVYSALQTGVIDGAENNWPSYFSTSH
                     YEVAKYFTLDGHTRVPEILIASKISMDKLSKEDQEIIKQAAKDSMPYQIKLWKEFEKV
                     SEEKVRAAGSIITELTPEAVVEFQNAMQPMYDALSPELQEVVKKIRDVQ"
     gene            1287..1808
                     /locus_tag="PK442_07945"
     CDS             1287..1808
                     /locus_tag="PK442_07945"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO87619.1"
                     /translation="MNAVTSFFRLLHTLLVILAKTLLAAMVLLIAANVFMRYVLNSGI
                     TWSEEVALVFVVWFTFIALALGVKQRLHISLCLLPEKISPKIDFLLSKLTDIVILFMG
                     YIMIHYGWILVQFTSRSILPATGVPASVLYFPLVLSGVLVLYEGGMNLLGLDKGDANM
                     DDKFSGRRCASDV"
     gene            1801..>2443
                     /locus_tag="PK442_07950"
     CDS             1801..>2443
                     /locus_tag="PK442_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO87620.1"
                     /translation="MSDPTIATWILLGSFALFLVLKVPITFSLAVSSVLTAIYLKIPL
                     MAIVQQMVQGVNSFSLLAIPFFIIAGEMMGEGGISRRLIAFSNLLVGRVRGGLAQVNV
                     LASMFFGGISGSAVADVSSIGTILIPMMKEKGYDADYSVAVTVTSACQGIIIPPSHNM
                     IIYSLAVGGVSVGRLFLGGFVPGVLLGIVLMIISYVIAVKRAYPKEKPYTLREA"
BASE COUNT          511 a          742 c          657 g          533 t
ORIGIN      
        1 ctgcgatctg ctcggatccg acgtggaacg cggggaactg ccggaggact acgcgcttct
       61 cggctctgta gttcaagatg tctgcttcaa caatgcaaag gaatactttc agctgaattt
      121 caataggtga aacaaggtcg aagagtgcgc gtattgtgta aaaaagcttg cttttgccct
      181 gcgaggcatt tacaataaat aggaatcgca ggttattcat caccacacta tgaaggagga
      241 gaaaaaatga agaagtggat tctggttctg acagcgcttg ctcttgtctt cgcggcactc
      301 ccggcggcgg ccgacaccgt cgtgctccgc ttgggagaga cgcacgtggc cgactatccg
      361 accacgaaag ggaattacga gtttgccagg ctcgtcgagg aacgcaccgg cggacggatc
      421 aagatcgaag tctaccacag cagtcagctc ggacaggaga aggcggtcat cgaaggagtt
      481 cagttcggag cgatcgattt cacccgcgtc agcatctccc cgctttccgc ctttgcgccc
      541 gctttcgacg ccctccagat gccgtatctg taccgcggcg aagagcacat gtggaaggta
      601 ctcaacggtt cgatcggaga ggagttcctg gccagcctcg aaccggcgaa cttcgtcggc
      661 ctcgcgtggt acgactccgg cgcccgaaac ttctataatt ccaagaagga gatcaaatcc
      721 gtcgccgatc tgaaagggat gaagatccgc gtccaggaga gccagctcat gatgggcctc
      781 gtatcgtctc tcggcgccgt ccccaccccc atgcccttcg gcgaagtgta cagcgcgctt
      841 cagaccggcg tcatcgacgg cgcggagaac aactggccca gctacttctc caccagccac
      901 tacgaggttg cgaagtactt cactctcgac gggcacaccc gcgttcccga gatcctgatc
      961 gcgagcaaga tcagcatgga caagctctcc aaagaagacc aggagatcat caagcaggcc
     1021 gcgaaggact ccatgcccta tcagatcaaa ctttggaaag agttcgagaa ggtctccgaa
     1081 gaaaaagtcc gcgccgcggg ttcgatcatc accgagctga cacccgaggc agttgtcgag
     1141 ttccagaacg ccatgcagcc gatgtacgac gccctcagcc ccgaactgca ggaagtagtc
     1201 aagaaaattc gcgacgtaca gtaacggctc gaaaagggaa gactcctgaa ggagtcttcc
     1261 ctttttttca ggaaggaggt ctgcccgtga acgctgtcac atcgttcttc cggctgctgc
     1321 acacgctcct cgtcattctc gcgaagacgc tgctcgcagc aatggtgctt ctcatcgccg
     1381 ccaacgtctt catgcgctat gtcctcaact ccggcatcac ctggtccgag gaggtcgcgc
     1441 tcgtcttcgt cgtctggttc accttcatcg ctcttgcgct cggcgtcaaa caacggcttc
     1501 atatcagcct ctgccttctt ccggagaaga tctccccgaa gatcgacttt ctcctctcca
     1561 aactgaccga tatcgtcatt ctcttcatgg ggtatatcat gatccactac ggatggatcc
     1621 tcgtccagtt caccagccgc tctatccttc cggcgacagg agttcccgcc tccgtactct
     1681 attttccgct cgtactctcg ggcgtgctcg tcctctacga aggcggaatg aatctcctcg
     1741 gactcgacaa gggcgacgca aacatggacg ataaattctc cggcagaagg tgcgcctccg
     1801 atgtctgatc cgaccatcgc tacgtggatc ctcctcggca gctttgccct cttccttgtc
     1861 ctcaaggtgc cgatcacctt ttccctggcg gtttcctccg tgctcaccgc gatatacttg
     1921 aagattcccc tcatggccat cgtccagcag atggtgcagg gagtgaattc tttctcgctg
     1981 ctcgcgattc cttttttcat catcgccggc gagatgatgg gcgagggcgg gatttcacgg
     2041 cggctcatcg cattctcgaa cctgctcgta gggcgcgtcc ggggcgggct cgcccaggtc
     2101 aacgtgctgg cgagcatgtt cttcggcggc atctccggtt ccgccgtggc ggacgtctcc
     2161 tccatcggca ccattctcat cccgatgatg aaggagaagg ggtacgacgc ggactactcc
     2221 gtcgcggtga cggtgacgag cgcgtgccag ggcatcatca tcccgccgag ccacaatatg
     2281 atcatctact ctctagcggt gggaggcgtc tcggtcggcc gccttttcct cggaggcttc
     2341 gtcccgggcg tgctgctcgg aatcgtcctt atgatcatta gctacgtaat cgccgtcaag
     2401 cgggcatatc cgaaggaaaa accctatacg ctccgggagg cta
//
LOCUS       DAOSWW010000372         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_33996_length_2436_cov_4.046619, whole genome shotgun sequence.
ACCESSION   DAOSWW010000372 DAOSWW010000000
VERSION     DAOSWW010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33996_length_2436_cov_4.046619"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK442_07955"
     CDS             <1..278
                     /locus_tag="PK442_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87621.1"
                     /translation="PGQPLPDGVTPPDFYEEGEGLMTWRNVVADNGPYDHNPAVGVVT
                     FRVCSVRAEAIQQQNGGGGGCSVGGAGSAWVAALLFVPVFALARKTR"
     gene            490..633
                     /locus_tag="PK442_07960"
     CDS             490..633
                     /locus_tag="PK442_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87622.1"
                     /translation="MEKKPLKNEGKIQEKDTKKKKKVDEMEESDLNGVAGGEGMDTGI
                     IKP"
     gene            713..2254
                     /locus_tag="PK442_07965"
     CDS             713..2254
                     /locus_tag="PK442_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOO87623.1"
                     /translation="MKRRNRFSHRIFLSLLCLLFLSIGSAEAATWTVTRTLDDGSTGT
                     LSHAVTHAAAGDSIVFAGNVTTVDLTSTLYLKKALTISGPATIRQTGANERVLHVTAD
                     CTLTSLTITGGNFVPGTHIGNYWNGAGIYNYANLTMNNCTVTGNTVGGGVYNVKNLTM
                     TGCTISNNTCAGSRHAGGINNEAKSSSAPVLTMYNCVVEGNSNPDTYGGGIFNGEKLF
                     MYGCTVRNNSSRGSGGGIYLNLFSETTLAQGCVVTGNSPDQICGPGQYGSLSIDGSCT
                     VGNTAGNKAVALSGFAQGSDPELRKTTGEPDVVAAEGALNDSGSGLFTSVKNTLHIDL
                     GASGEVVAGLASMSATLYNAFTYENVQLADASGEGELVIEFTASWPERVRYYAAFAEY
                     EGVAAGTLAVKGYVVAERGVQFETKPGQPLPDGVTAPDFYEEGEGLMTWRNVVTDNGP
                     YDHNREDGVVTFRVCSIRAEAVQQQGGGGGGGCSVGGGSAWTAALLLFVPLVVNGCGK
                     RRRRS"
BASE COUNT          524 a          669 c          811 g          432 t
ORIGIN      
        1 agccggggca gccgctcccc gacggcgtaa cgccgcccga cttctacgag gagggcgagg
       61 ggttgatgac gtggcggaac gtggtggcgg acaacggtcc gtacgaccac aatcctgccg
      121 tgggcgtcgt gaccttccgc gtctgttccg tccgcgcgga agccatccaa cagcagaacg
      181 gcggaggggg aggctgctct gtcggcggcg ccggaagtgc gtgggtcgcc gccctgctct
      241 tcgttccagt cttcgctctc gcgaggaaaa cgagatagaa ccttgagaga aacgactcat
      301 caaagagaca tcctctgctt gcagagcctg tgtgcggatg tacagaccga cttttgagaa
      361 aaaagagaag atttatatgc ttgccccttg actttaccat agtgtcaagg tttatcatct
      421 ctctatagac ggaaacgtcg ggcgatgcgc ggtgcggtga atgtactcaa aacgatggag
      481 gtgttcggga tggaaaagaa accgctcaaa aacgaaggga agatccagga gaaggatacg
      541 aagaaaaaga agaaggtcga cgaaatggaa gagagcgatc tcaatggagt ggccggcggc
      601 gaaggaatgg atacggggat catcaagccc tgatctttgt ggacggacgg agggttggaa
      661 cagcgccctc cgcctttttc tgttcaagct tctttcgaaa gggtgatcga cggtgaaacg
      721 aagaaaccgc ttttctcata gaatattcct ctctctgctc tgcctgctct ttttgagcat
      781 cgggagcgcg gaggccgcga cgtggacagt gaccagaacg ctcgacgacg gttccacagg
      841 gacgctgagc cacgcggtga cgcacgccgc tgcgggcgat agtatcgtat ttgctggaaa
      901 tgtcacgacg gtcgatctga cctcgacgct ttatctcaag aaggcgctca ccataagcgg
      961 ccctgcgacg atccgccaga cgggggcgaa cgagcgcgtg ctccacgtaa cggccgactg
     1021 cacgctcact tcgctgacca tcaccggcgg gaacttcgtt ccggggacgc acatcggaaa
     1081 ttactggaac ggcgcaggaa tatacaacta cgcgaacctg acgatgaaca actgcaccgt
     1141 gacgggcaac accgtcggcg ggggcgtgta caatgtgaag aatctgacca tgaccggctg
     1201 cacgatcagc aacaacacct gcgcaggatc gcggcacgcg ggcggcatca acaacgaggc
     1261 taaatcatcc agcgctccgg tactgacgat gtacaactgc gtcgtggagg ggaactcgaa
     1321 ccccgatacc tacggaggcg gcatcttcaa cggggagaaa ctcttcatgt acgggtgcac
     1381 ggtgcggaac aacagcagca gggggtcggg aggcggtata tacctcaatc tcttcagcga
     1441 gacgaccctg gcccaggggt gcgtggtgac ggggaactct cccgaccaga tctgcgggcc
     1501 ggggcagtac ggctcgttga gtatcgacgg ttcgtgcacc gttggaaaca cggcggggaa
     1561 caaggccgtc gcgctctccg gcttcgcgca gggaagcgat cccgaactcc ggaagacgac
     1621 cggcgaacct gacgtcgtcg ctgcggaggg tgcgctgaac gactccggga gcggcctgtt
     1681 cacgtcggtg aagaatacgc tgcatatcga cctcggcgct tcgggggagg ttgtcgccgg
     1741 ccttgcgagc atgagcgcga cgctgtataa cgccttcacc tatgagaacg tgcagcttgc
     1801 cgacgcgtct ggcgagggag agctggtcat cgagttcacc gcctcgtggc cggagcgcgt
     1861 ccgctactat gcggcgttcg ccgagtacga gggcgtggcg gctggtactc tcgccgtgaa
     1921 ggggtacgtc gttgccgagc gcggcgtcca gttcgagacg aagccggggc agccgctccc
     1981 cgacggcgta acggcgcccg acttctacga ggagggcgag gggttgatga cgtggcggaa
     2041 cgtggtgacg gacaacggcc cgtacgacca caaccgcgaa gacggcgtcg tgaccttccg
     2101 cgtctgctcc atccgcgcgg aggcggtgca gcagcagggg ggcggcggcg gaggaggatg
     2161 ctcggtcggc ggcgggagtg cgtggacggc cgcgctgctg ctctttgttc ccctcgtcgt
     2221 caacggatgc ggaaagcgca gacggcgctc ataaggttgt tcgtcgggta cctatgaaat
     2281 atacttgacg cgcgtgttcg aaaaggggat tcgcgatgaa ggagaaagag gtccgctact
     2341 atttcgcgga acagcggggg ctggaagttg tgcgcctgaa gggatcgcgc ttcttctacc
     2401 cggaacacag tcatgtgtcc acctatacgg cgagcc
//
LOCUS       DAOSWW010000373         2434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34041_length_2434_cov_4.548129, whole genome shotgun sequence.
ACCESSION   DAOSWW010000373 DAOSWW010000000
VERSION     DAOSWW010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34041_length_2434_cov_4.548129"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PK442_07970"
     CDS             complement(<1..435)
                     /locus_tag="PK442_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOO87624.1"
                     /translation="MIVFPAMDLYGGEIVRLRQGKFSDRTVYPAKIAETAKSFLEAGS
                     EWIHLIDLQGAEEGAPRHLSVVGRLKETGLLVQYGGGLRSEEDIAAAFEAGADRLYLG
                     SILVKDRFLAGRMYDAYGSKVIPAVDIRNGDVVISGWKEAGGI"
     gene            complement(432..1055)
                     /gene="hisH"
                     /locus_tag="PK442_07975"
     CDS             complement(432..1055)
                     /gene="hisH"
                     /locus_tag="PK442_07975"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOO87625.1"
                     /translation="MIAVVDYQVGNTGNVRRAFERLGEQVCLLHSPDELGDRVSVAVL
                     PGVGAFGPASERLAASGWRETLQSWARSGRPLLGICLGMQLLCEKGLEDGEYEGLGLI
                     PGTALPLAASKIPHMGWNTVEWTGRPPSFLPSAPDGSFFYFVHSFALPEGEFTTGRTT
                     LEGNVFSSMVLKGSVAGFQFHPERSGPEGVALLGETVRFLRKEGAPQ"
     gene            complement(1052..1618)
                     /locus_tag="PK442_07980"
     CDS             complement(1052..1618)
                     /locus_tag="PK442_07980"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HOO87626.1"
                     /translation="MIEKERTTKETSVSLRLDVDGGDRRIEIPCGFLRHMLELFAFHA
                     GIGLDVTATGDVDVDCHHVTEDVGILLGKAFEQARSGRNIQRYGWCALPMDGSLVLAA
                     VDFSGRGQFEWKGAFPAERCGDFDLELLPEFWRAVCREGRITLHAQALACDNAHHLAE
                     ALFKGAGRAFRQALSPAGELQSTKGTLA"
     gene            complement(1615..2259)
                     /gene="hisG"
                     /locus_tag="PK442_07985"
     CDS             complement(1615..2259)
                     /gene="hisG"
                     /locus_tag="PK442_07985"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOO87627.1"
                     /translation="MLTIALPTGRVFSEAAELLMAAGLPASSLASPGRKLVFEEENFR
                     YILAKPSDVPLYVSYGAADLAFAGGDVLQESALPLVEILDTGRGRCRVVVAGPASLAE
                     RFRGHASERMWLKVATKYPRIADNHFSSKGAQVDIIHLHGSIELAPKLGLSDCILDIV
                     QTGSTLKANHLEVLEHVSDVSLRLVASKKSVALRWADIRAVSRAIKSRCKEVRS"
     gene            complement(2253..>2434)
                     /locus_tag="PK442_07990"
     CDS             complement(2253..>2434)
                     /locus_tag="PK442_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87628.1"
                     /translation="LATGGRPLEMSWCADRASSLENAAQRGCSSWIDLSDGSTLDLAT
                     QGDGRTLPGKENFRC"
BASE COUNT          437 a          804 c          758 g          435 t
ORIGIN      
        1 gatgccgccc gcctctttcc agccggagat caccacatcg ccgttgcgga tgtctaccgc
       61 ggggatgacc ttcgacccgt aggcgtcgta catcctgccc gcgaggaaac gatccttcac
      121 gaggatgctt cccagataga gcctgtcggc gcccgcctcg aaggctgcgg cgatgtcctc
      181 ctcggatcgg agtcctccgc cgtactgaac aaggagccct gtttccttga gacggccgac
      241 aacggaaagg tggcgcggcg ctccctcctc ggcgccttgc aggtcgatca ggtgaatcca
      301 ttccgaccct gcttcaagga agctcttcgc cgtctcggcg atctttgccg gatacacggt
      361 tctgtcgctg aactttccct gcctgaggcg gacgatttcg cctccgtaga gatccatcgc
      421 gggaaaaacg atcattgcgg ggcgccctcc tttcgtagaa acctgacggt ctcccccagc
      481 agcgcgacgc cttcggggcc gctgcgctcc gggtgaaact ggaatcccgc cacggagccc
      541 ttcagcacca tcgacgagaa gacatttcct tcgagcgttg tccgccctgt cgtgaattca
      601 ccttcgggga gcgcgaagct atggacgaag tagaagaacg agccgtcagg cgcggacggt
      661 aaaaacgacg gcggtcgacc ggtccattct acagtgttcc accccatgtg aggaattttg
      721 gacgctgcaa gggggagcgc ggttcccggg atcagtccga ggccttcata ttccccgtcc
      781 tcaagcccct tttcgcacag aagctgcatc ccgaggcaga tgccgagcag cggccgcccg
      841 gaacgcgccc acgattgaag tgtttcgcgc caccccgagg ctgcgagtcg ttccgaagcc
      901 ggtccgaatg ccccgactcc cggaagcacg gcgaccgaaa cgcggtcgcc gagttcgtcc
      961 ggagagtgga gcagacagac ctgctccccg aggcgctcaa aggcgcggcg gacattaccc
     1021 gtgtttccca cctggtagtc cacgacggcg atcatgcgag cgttcccttc gtgctttgca
     1081 gctctccggc gggagagagg gcctgccgga acgccctgcc cgctcccttg aagagcgcct
     1141 ctgcaagatg gtgtgcgttg tcgcaggcga gggcctgcgc gtggagcgtg atccttcctt
     1201 cgcggcagac cgcgcgccag aactcgggca gaagctcaag gtcgaagtcg ccgcaccgct
     1261 cggcagggaa cgctcccttc cattcgaact gcccccggcc gctgaaatcg acggcggcca
     1321 gcacaagaga gccgtccatg ggaagagcgc accagccgta gcgctgaatg ttccggccgg
     1381 aacgggcctg ttcgaacgcc tttcccagca ggataccgac gtcctccgtc acgtggtgac
     1441 agtccacgtc gacgtctccg gtcgccgtca cgtcgaggcc gatcccggcg tgaaaggcga
     1501 agagctccag catgtggcgc agaaagccgc acggaatctc gatgcgccgg tctccgccgt
     1561 caacatccaa gcgcagagag accgaggtct cctttgtcgt ccgttctttc tcgatcatga
     1621 ccgaacctcc ttgcaccggc ttttgatcgc cctgctcacc gcccggatat cggcccagcg
     1681 gagggcgacg ctcttcttgc tcgccaccag gcggagagag acgtcggaga cgtgctccag
     1741 cacttcgagg tggttggcct tcagtgtact gccggtctgg acaatgtcaa ggatacaatc
     1801 gctcagtcca agcttcggcg ccagttctat ggagccgtgg aggtggatga tgtccacctg
     1861 cgcgcccttg gaggagaagt ggttgtccgc gatgcgcggg tacttggtgg ccaccttgag
     1921 ccacatccgc tcggaggcgt gcccccggaa tctctcggca agcgaggcgg gcccggcgac
     1981 gacgacgcgg cagcgccctc tccccgtgtc gaggatttca acgagcggca gcgcgctctc
     2041 ctggagaacg tcgcctcctg cgaaggcgag gtcggctgcc ccgtagctca catagagcgg
     2101 cacgtccgag ggtttcgcca ggatgtagcg gaagttctcc tcttcaaaga cgagcttgcg
     2161 tcccggcgag gcaagggatg acgccggcag tccggcggcc atgagcagct ccgccgcttc
     2221 ggagaagacg cggcctgtgg gcagggcgat ggtcagcatc ggaaattctc ctttccgggg
     2281 agcgtccggc catccccttg cgttgcaaga tcaagcgtcg agccgtccga gagatcgatc
     2341 caggaggagc acccccgctg cgcggcattt tcgagggagg aagcccggtc ggcacaccag
     2401 ctcatttcga gcggcctccc tcccgtcgcc aggc
//
LOCUS       DAOSWW010000374         2425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34240_length_2425_cov_3.848523, whole genome shotgun sequence.
ACCESSION   DAOSWW010000374 DAOSWW010000000
VERSION     DAOSWW010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34240_length_2425_cov_3.848523"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK442_07995"
     CDS             <1..399
                     /locus_tag="PK442_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOO87629.1"
                     /translation="REIVAGLDVKKIELGVRQQAITDAFQAALKTLASMPGEAYISFI
                     GKLFKKAVETGNEKVYISKSEKNISSEWISKLNAQNGWSLSLSEERRDISGGFILEQD
                     NIETNCSFDMLVRWIRDEIEADVVKRLFSA"
     gene            416..1429
                     /locus_tag="PK442_08000"
     CDS             416..1429
                     /locus_tag="PK442_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOO87630.1"
                     /translation="MAPAERFAYAVARLRAMSGRLLEEALIQRILDSEDLDSALKVLG
                     ETTYSSWLVELKGGADFDRVIESELLHVYTEVQKFVPDPRLVHLCRIPYDFHNVKVLL
                     KSLILAREGRERRFDLLTSLGVLPTDDLIMAMESEDYRLLPFGLHRLVPLCLSHWEQT
                     KDILQVENILDDGLFSVLSEMAAATGFDSATRWVKGRIDAENIRNLLRLKRMEMDTGN
                     IASFLHGGGWISREKLLALLPEPVESWPRILSFADVASAFSHIQDLSDLGVLLVEMEK
                     VLDEYVSSILARAKYDAFSPENVIAFLWMKELEAKNLRIAMVSVANNTDRNLARGLLR
                     HGR"
     gene            1419..1760
                     /locus_tag="PK442_08005"
     CDS             1419..1760
                     /locus_tag="PK442_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOO87631.1"
                     /translation="MAVKKTGSPMAAVGEYETVLPFQAVGVKPCIVKDDETSDVGAIL
                     LELAKAGFAVVFVQESFFVGHTRRIEELNDEYETSFIPIPGLKGSEGVGLESIRSSVE
                     RAVGMDIFAVK"
     gene            1788..>2425
                     /locus_tag="PK442_08010"
     CDS             1788..>2425
                     /locus_tag="PK442_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOO87632.1"
                     /translation="MAAEKVIKGTIEKISGPLVVAKGMFGAGMYDVVRIGEIGLVGEI
                     IELKGDTASIQAYEETSGLMPGERVVSTGEPLSVELGPGLIEQFYDGVQRPLNLIEDA
                     AKSHYISRGIDVPAIDRKKKWEFTPKVKAGDAVEQGDILGVVQESVLVEHRIMVPVGL
                     GGKVVKIEKGSFTVEEVVAVLEQNGEKKNVTMLQRWPVRKARPVAKRLPPVIP"
BASE COUNT          610 a          534 c          681 g          600 t
ORIGIN      
        1 cgagagatcg tcgccggcct tgatgtgaaa aaaatcgaac ttggagtcag gcagcaggct
       61 ataacggatg cttttcaagc agcactgaag acgctcgcgt ccatgcctgg agaagcatac
      121 atttctttta tcggcaagct gttcaaaaaa gcagtggaga cgggaaatga aaaagtgtat
      181 atttcgaagt ccgaaaaaaa catctcttcc gagtggattt cgaagctcaa tgcccagaat
      241 ggatggtcgc tttctctgag tgaggaacgc cgggatatat cgggtggatt cattttggaa
      301 caggacaata tcgagacaaa ctgttcgttt gatatgcttg tccggtggat tcgggacgaa
      361 atcgaggcag acgttgtcaa gcggctgttt tcggcgtgat cggaggtgac gctccatggc
      421 tccggctgag cgttttgcct atgcggtcgc gcgactccgg gcaatgtccg gacgtcttct
      481 agaagaagcg ttgatccaac ggatactgga cagtgaagac ctcgattcag cactgaaagt
      541 tctgggcgag acgacatact catcttggct cgttgaattg aaaggcggag ctgacttcga
      601 cagagtaatc gaatccgagc ttttgcatgt gtataccgaa gtgcagaaat tcgtgcccga
      661 tcctcgtctt gtgcatctgt gccgtattcc ttatgacttt cataatgtga aagtactttt
      721 aaaaagtttg attcttgcac gagaaggccg agagcgccgg tttgatcttt taacttctct
      781 tggcgttctc cccacggatg atttgattat ggcaatggag agcgaagact accgtcttct
      841 cccctttggc cttcatcgtc ttgttccgtt atgcctttcg cattgggaac agacgaaaga
      901 tattcttcaa gtcgagaaca ttcttgacga cggtcttttc tctgtcttat ccgaaatggc
      961 ggcagcgaca ggttttgact ccgctactcg ttgggtaaag ggcaggatcg acgctgagaa
     1021 tattcgtaac cttcttcggt taaagcgtat ggaaatggac acggggaata ttgcatcatt
     1081 tctgcatgga ggcggctgga tatcgcgaga gaaactcctc gctctgcttc ctgaacccgt
     1141 ggaaagctgg ccgagaattc tttctttcgc ggacgtggca agcgctttct cacatatcca
     1201 ggacctttcc gaccttggcg tcctcttggt ggagatggaa aaagttcttg acgagtatgt
     1261 gtcaagcatt ctcgcaagag caaaatacga tgcgttctcg ccggaaaatg tgatcgcctt
     1321 cctttggatg aaggagcttg aggctaagaa tctccgtatt gcgatggtct ctgtagcgaa
     1381 taatacggac agaaatcttg cgagggggtt acttcgtcat ggccgttaag aaaacaggtt
     1441 ctccaatggc cgctgtcggc gagtacgaaa cagtccttcc ttttcaagcg gtcggagtga
     1501 agccgtgcat tgtcaaggat gatgaaacct cggacgtcgg tgcaattctt cttgagcttg
     1561 caaaagcagg ttttgcggtc gtcttcgtcc aagagtcttt cttcgtcggg cacacccgta
     1621 ggatcgaaga actgaacgac gagtacgaaa caagtttcat ccccatcccc gggctaaaag
     1681 ggagcgaggg agtcggtttg gaatccattc gaagcagcgt cgagcgagca gtcggaatgg
     1741 atatttttgc agtgaagtaa tgaatagaag tttggaggcg atagattgtg gccgctgaga
     1801 aagtcataaa aggaactatc gaaaaaatat ccggccccct tgtagtggcg aaaggtatgt
     1861 tcggcgccgg catgtacgac gtggtgcgga tcggcgagat cggactcgtc ggagaaatca
     1921 tcgagcttaa gggcgatacg gcatctatac aggcgtatga agagacctcc ggccttatgc
     1981 ccggagagag ggttgtcagt acaggggaac ctttgagcgt cgaactgggg cccggcctta
     2041 tagaacagtt ctacgacggc gtacagagac ctttgaatct gatagaagac gcggcaaaga
     2101 gtcattatat ctcccgaggt atcgacgtac ccgccatcga cagaaagaaa aaatgggagt
     2161 tcaccccgaa agtaaaagcg ggcgacgcag tggaacaggg cgatattctt ggcgttgtgc
     2221 aggagtcggt tctcgtggag caccgcatca tggtgccggt aggcttgggt ggcaaagtag
     2281 tcaagattga aaaaggctct ttcactgtag aggaagttgt cgcggttctt gaacagaacg
     2341 gcgagaaaaa gaacgtaaca atgcttcaaa ggtggccggt tcgaaaagca cgacccgttg
     2401 cgaagcgtct tcctcctgtt attcc
//
LOCUS       DAOSWW010000375         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34504_length_2413_cov_4.377014, whole genome shotgun sequence.
ACCESSION   DAOSWW010000375 DAOSWW010000000
VERSION     DAOSWW010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34504_length_2413_cov_4.377014"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..483)
                     /locus_tag="PK442_08015"
     CDS             complement(142..483)
                     /locus_tag="PK442_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO87633.1"
                     /translation="MGSIRSRQSNPPFRMRVYGLLLFVFLILCAACFPTDLFAAKIVR
                     VGLYENSPKIFTAESGLPAGIFADIIRYIAEKEGWELRFVHGTWSQGLDRLEKGYIDL
                     MPDVAFTEARG"
     gene            complement(665..1405)
                     /locus_tag="PK442_08020"
     CDS             complement(665..1405)
                     /locus_tag="PK442_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO87634.1"
                     /translation="MKVFLVMAFCLFSVSAWALTVDDVTFMTEDYPPFNMAGADGTAT
                     GASVEVLVGIFEKLGAAKTVKDIQVLPWARGYREVQNTPNTCLFLMTFTDERKPLFKW
                     VGPLAPTKVGVLALKSKGLKITSASDLANLKAGVVRDDIGDELAKQAGIQQIERVPSN
                     DQNIKKLTEGRIDIWVFEESVAKWQIQSLGFNADDYESVWTLSESDLYYAFHKDTDDA
                     LIASIQKTLDDMKADGSYEAVMKKYNMR"
     gene            complement(1636..2385)
                     /locus_tag="PK442_08025"
     CDS             complement(1636..2385)
                     /locus_tag="PK442_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO87635.1"
                     /translation="MKLLKIALVLVFCLCSGSAWALTVDDITYMTEDYPPFNMAGADG
                     VATGAAVDTLTTIFERMGAAKTAKDIQVLPWARGYREVQSTPNTCLFLMTLTDERKPL
                     FKWVGPVVVNTFDAIALKSKGMKIASAEELVNLKAGVIRDDVGDALAQKAGIKQIERV
                     PSNDQNIKKLNEGRIDIWVFGESSAKLQVKEMGLNPDDYESVWRMSESGLYFAFHKDV
                     DDALIESMQKVLDEMKADGAYEAIMKKYNVQ"
BASE COUNT          535 a          680 c          613 g          585 t
ORIGIN      
        1 gaaaatctcc tgctggatgg aacggtagag cacggcgacc cgtttgccgt cgaggtctgg
       61 aatcgagcgg atgccgcttc tgcggcgtga aaaaacctgg aaccagctgt acaacgccgg
      121 gtcggagtgg aaggtaaaaa tctatccgcg cgcctccgta aacgccacgt cgggcatcag
      181 gtcgatgtag cccttttcga gacggtcgag cccctgactc catgttccgt ggacgaaccg
      241 gagctcccac ccctcttttt cggctatgta cctgatgatg tccgcaaaaa tgcctgcggg
      301 aaggccggat tccgcagtga aaatcttcgg gctgttctcg tagaggccga cgcggacaat
      361 tttcgctgca aagaggtctg tcggaaaaca tgccgcacaa aggatgagaa atacaaacaa
      421 aaggagaccg tatacgcgca tccggaacgg cggattactc tggcgactgc gtatgctgcc
      481 cataagacca gcctcctctt tcttttgttc ccaaacatag aatacactac tttcaccgaa
      541 aggcaaagag aagagcagag ggcgagtaac tcttttttct tccccgtcgc ccttaccaac
      601 cggggggaca aaaaagaccg ccccgggttg aggggcggcc ctattgtcgt cggaagagag
      661 agggttaccg catgttgtac ttcttcatga cagcttcgta cgagccgtcg gccttcatat
      721 cgtcaagcgt cttctgaatc gacgctatga gggcgtcgtc agtgtccttg tggaatgcgt
      781 agtagaggtc gctttcgctg agcgtccaga cgctctcgta gtcgtcggca ttgaagccaa
      841 ggctctgaat ctgccatttc gccacgcttt cttcaaaaac ccatatatcg atccggcctt
      901 cggtcagctt cttgatgttc tggtcgtttg aaggaactcg ttcaatctgt tgaatacccg
      961 cctgcttcgc cagttcgtcg ccgatatcgt ctctgacgac cccggccttc aggttggcca
     1021 gatcgcttgc ggaagtaatt ttcagcccct tgcttttcag cgccagcacg ccgaccttcg
     1081 tcggagcgag cggtcccacc catttgaaca gaggtttgcg ttcgtccgtg aacgtcataa
     1141 ggaaaaggca tgtgttcggg gtgttttgta cttcacggta tcctctcgcc cacgggagca
     1201 cctggatatc ctttacagtc ttcgccgccc ccagtttctc aaagatgccg acgagtacct
     1261 caacggacgc tcctgtcgcc gttccgtccg ctcccgccat gttgaacgga ggataatcct
     1321 cggtcatgaa cgtaacatca tccaccgtca gcgcccacgc gcttaccgag aacaagcaaa
     1381 aagccattac gagaaaaacc ttcatcaatt tcactatgtt tcccctcctc ttggtgtttt
     1441 gggcgccggt ttcgattgat tcattcgatc cggagcagta tagaataaaa gagtgattgc
     1501 atggtataaa taaaatatat caaaattgct aaaaaagtca aggtttttta tctggggcgt
     1561 tggcgaaaag aaaacgaaac aaggccgccc ggattgaggc ggccttgtca ggtacaattt
     1621 agaaagcgtt ttttgctatt gtacgttgta cttcttcatg attgcttcgt acgcgccgtc
     1681 ggccttcatc tcgtcgagga ccttctgcat cgactcgata agcgcatcgt cgacgtcctt
     1741 atggaaggca aaatagaggc cgctctcgct catacgccac acgctttcgt agtcgtccgg
     1801 attcagcccc atctccttga cctggagctt cgcgctgctt tctccgaaga cccagatgtc
     1861 gatgcggcct tcgttcagct tcttgatatt ctggtcgttg gaaggcactc tttctatctg
     1921 cttgataccg gctttctgag cgagcgcgtc gccgacatcg tcacggatga ctccggcctt
     1981 gaggttgaca agctcctcgg cggaggcgat cttcattccc ttgctcttga gggcgattgc
     2041 gtcgaacgtg ttgacgacga ccggaccgac ccacttgaac aggggtttgc gctcgtccgt
     2101 gagagtcatc aggaagaggc aggtgttcgg cgtgctctga acttcccggt atccccgggc
     2161 ccagggaaga acctgaatgt ccttcgccgt cttggccgcg cccattctct cgaagatggt
     2221 cgtcaaggta tccactgccg cgcccgttgc cactccgtcc gcccccgcca tgttgaacgg
     2281 aggataatcc tcggtcatat acgtaatatc atccactgta agagcccatg cgctccctga
     2341 acaaagacaa aagaccagta caagagcaat cttcaacaac ttcacgacgt atcccctccc
     2401 aaaattatgg aat
//
LOCUS       DAOSWW010000376         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34506_length_2413_cov_4.043681, whole genome shotgun sequence.
ACCESSION   DAOSWW010000376 DAOSWW010000000
VERSION     DAOSWW010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34506_length_2413_cov_4.043681"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..518
                     /locus_tag="PK442_08030"
     CDS             273..518
                     /locus_tag="PK442_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOO87636.1"
                     /translation="MPLNELAVPNRVTGERQVRRTLAGGRLRKLFIAEDADPSVIKNL
                     EEEARRQNIPVERVDSMLRLGRACAIARGASAAGIEG"
     gene            612..983
                     /gene="rpsL"
                     /locus_tag="PK442_08035"
     CDS             612..983
                     /gene="rpsL"
                     /locus_tag="PK442_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOO87637.1"
                     /translation="MPTINQLIRHGREEKRRKSDSPALQGNPARRGVCTRVYTVTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIIRGTL
                     DCGGVENRKRSRSKYGARKPK"
     gene            1028..1498
                     /gene="rpsG"
                     /locus_tag="PK442_08040"
     CDS             1028..1498
                     /gene="rpsG"
                     /locus_tag="PK442_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOO87638.1"
                     /translation="MPRKGHVKKRQATPDTKFGSPALGKFINSLMRKGKKSVAERIMY
                     NALDIAGEKLQTEPFEVFKKAMENVRPLVEVRPRRVGGATYQVPVEVPAERAQVLSIR
                     WILNYSRSKKGMPMAERLAREFMDACKNEGASIKKKEDTHKMAEANRAFAHYRW"
     gene            1563..>2413
                     /locus_tag="PK442_08045"
     CDS             1563..>2413
                     /locus_tag="PK442_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO87639.1"
                     /translation="MQKLDMKSIRNIGIAAHIDAGKTTTTERILFYTGKNYKMGETHE
                     GAATMDYMDQERERGITITSAATTCMWHDHYINIIDTPGHVDFTVEVERSMRVLDGAV
                     AVFCAVGGVEPQSETVWRQADKYHVPRVAFVNKMDRVGADFENVVRGIKEKLGAKPLP
                     VQIPIGVEDSFTGMIDLVEFHGIQYTDDMGTDPHVVPIPSALADEAKAARESMLETLS
                     DYDEQIMEAFLDGGDVPVETIKRAIRAATVALKLVPVICGSAFKNKGVQFLLDAVVDY
                     LPSPVDLP"
BASE COUNT          603 a          604 c          725 g          481 t
ORIGIN      
        1 cggagccgca gtgaagggag agatcgatcc ccttgaagga ctgaaggaaa acgtcatcat
       61 cggacatctc attccggccg gaacaggagt tgaacgattc cagggggttc gcacatacat
      121 tcagcacccc gaaaaggaag aatagctctt cggcgtcttt gctccagagt gagaaaatgt
      181 aaatactctg aaggcgggag cattttatgc ttgacattgc tcccgccttt ttgttattat
      241 tccgtggtgc ttttggcaca ggagggattt tattgccttt aaatgaactc gcagtcccga
      301 acagagtgac gggagaacgt caggtacggc ggacgcttgc cggcggacgg ctcaggaagc
      361 tctttatagc ggaagatgcg gacccttcag tgataaaaaa cctcgaagaa gaagcgcggc
      421 ggcagaatat tccggttgaa cgcgtcgaca gtatgctccg gctcggaaga gcctgcgcca
      481 tcgcacgagg agcttcggcg gctggaatag aaggatagat gggcgaatat ccggaagacg
      541 agggaaaagg gtttttcgga aaaaagtttc tttgcccaga atacgaagaa cagatggaag
      601 gaggaagttg cgtgccaacg atcaatcagc ttatacggca tggccgggaa gaaaagaggc
      661 gcaagagcga ttcgcctgcg ctgcagggaa accccgcccg gcggggtgtc tgcacgcgtg
      721 tgtacacggt gacgccgaaa aagcctaact cggctctgcg gaaggttgcc cgagtccgcc
      781 tcaccaacgg tatcgaggtt acctcgtaca ttccgggaat cggccacaac ctgcaggagc
      841 actcggtggt tcttgttcgt ggcggaagag tgaaagacct tcccggagtc cggtatcaca
      901 ttattcgggg aacactcgac tgtggaggcg tggagaatcg aaagagatcc cgctccaagt
      961 acggcgcccg gaaacccaag tagggttttg cggcgtgttt tttgttattt tgaggagggg
     1021 ttgaccaatg ccgcgtaagg ggcatgtcaa gaaacgccaa gcgacgccgg ataccaagtt
     1081 cggcagcccc gcactgggca agttcatcaa cagcctgatg cggaagggaa agaagagcgt
     1141 tgccgagcgg attatgtaca acgcgctcga tatcgccgga gagaagctcc agacggagcc
     1201 cttcgaggtc ttcaagaagg ccatggagaa cgtccgtcct ctcgtcgagg ttcgcccccg
     1261 ccgcgtcggc ggagcgacct accaggtacc ggtggaagta cccgccgaaa gagcgcaggt
     1321 gctctccatc agatggatcc tgaactattc ccggagcaag aagggaatgc ccatggctga
     1381 aagactcgcc agggagttca tggatgcctg caagaacgaa ggcgcttcca tcaagaagaa
     1441 ggaagatacg cacaagatgg cggaggctaa ccgcgcgttc gcccattacc gctggtaata
     1501 aagtcgttca cagattcaat acgatgcagt gctctgcagg aaacgaggct tggtggtagg
     1561 aaatgcaaaa acttgatatg aaaagtatcc gcaacattgg aatcgccgcc catattgacg
     1621 cgggcaagac gacgaccact gagcgaattc ttttctacac cggaaaaaac tacaaaatgg
     1681 gcgaaacgca cgaaggcgcc gccaccatgg actacatgga ccaggagcga gagcggggga
     1741 ttaccattac ctccgctgct acgacgtgca tgtggcacga tcattatatt aatataattg
     1801 atacgcccgg gcacgtggat ttcaccgttg aggtcgagcg ttcaatgaga gtcctcgacg
     1861 gcgcggtagc ggtcttctgt gctgtcggcg gagtcgagcc gcagtccgag acggtctggc
     1921 gtcaggcgga caagtaccac gtgccgagag ttgccttcgt gaacaagatg gatcgtgtcg
     1981 gggccgattt tgagaacgtc gtccggggca tcaaggaaaa gcttggagcc aagccgcttc
     2041 ccgtccagat tccgatcggc gtcgaggaca gctttacagg aatgatcgat cttgtcgaat
     2101 ttcatggtat ccagtacacg gacgatatgg gaaccgatcc tcacgttgta cctattcctt
     2161 cagcgcttgc cgacgaggca aaggccgcgc gtgaatccat gctggagact ctttcggatt
     2221 atgacgagca gatcatggaa gcttttctcg acggggggga cgtccctgta gaaacgatca
     2281 agagagcaat tcgcgccgct accgtcgcac tgaagctcgt tccggtcatc tgcggatcgg
     2341 cgttcaagaa caaaggggtt cagttcctgc tcgacgctgt tgtggactat cttccgagtc
     2401 cggtggacct tcc
//
LOCUS       DAOSWW010000377         2413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34516_length_2413_cov_2.395674, whole genome shotgun sequence.
ACCESSION   DAOSWW010000377 DAOSWW010000000
VERSION     DAOSWW010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34516_length_2413_cov_2.395674"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..455)
                     /locus_tag="PK442_08050"
     CDS             complement(171..455)
                     /locus_tag="PK442_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HOO87640.1"
                     /translation="MEATRSTREHSDEKEVFVFTGKLSFRRREAFRIARKAGHDVSGG
                     FTSRATCLVAGRLSRISRKGREASRRGVRVISEDEFLRMTGAPRQLHIEY"
     gene            1030..1482
                     /locus_tag="PK442_08055"
     CDS             1030..1482
                     /locus_tag="PK442_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87641.1"
                     /translation="MLDIGDYRENVALMLDISILGPLPCTLFFLFAMPAAEMSMLFQN
                     TLYMKDVGYSFVASFLRERFSPFLVTTDHLPLLLAQYFLLAGRFLPAFGLIAFANLQI
                     SLRQSILHSLETEGNEKDGSAVRAVNGLLNLLFLLPLTAFLLKALGAA"
     gene            1526..1894
                     /locus_tag="PK442_08060"
     CDS             1526..1894
                     /locus_tag="PK442_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87642.1"
                     /translation="MIEREFGMEDVLSFLTGILMRENLSDLVEIASFVLERKVSLKEL
                     PFIADEIRRRILKQHPWLRSLEEEDGECVEIAEWVRSVEAKYGGTILLKAEDFSKKNR
                     FRRLRERAAKLLFKTERLGF"
     gene            1921..2073
                     /locus_tag="PK442_08065"
     CDS             1921..2073
                     /locus_tag="PK442_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87643.1"
                     /translation="MRQTSRETLSKPGVKEGDILQDGSLRSPSSYVVALLQQHRLRIF
                     FSPVFN"
     gene            2159..>2413
                     /locus_tag="PK442_08070"
     CDS             2159..>2413
                     /locus_tag="PK442_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87644.1"
                     /translation="MREKKAGCTAEEENEPETYMLREGVGLFVDYFRNSFPDLEEYRV
                     RYLPVIEMLAASTPPLPERAVGEILHRGRTETRALLNELGP"
BASE COUNT          565 a          646 c          659 g          543 t
ORIGIN      
        1 cccgtacggc tggaagcgag ccgcaacccg ttcgatctcg ccaagtctta aaaactcgtt
       61 ttcgatagaa taaaactcgg aaacgagttt aatcttgttt gtgctccctc ctccgttaga
      121 tattatcgca gccgctgaca attaaaaaat cgcaaaaaac gagttcagta tcagtactcg
      181 atatgaagct gccgaggagc accggtcatc ctcagaaatt cgtcctccga aatgacacgc
      241 acacctctcc gagaagcctc gcgacccttt cgggatattc ttgagagtcg tccggcgaca
      301 aggcaggtcg cacgggatgt gaacccgccg gagacgtcgt gccccgcctt cctggcgatt
      361 ctgaacgcct ctcgtctcct gaaagaaagc ttgccggtaa aaacaaaaac ctccttctca
      421 tcggagtgtt cccgtgtcga tctcgttgct tccatgacga ccacctccca acctttcgtt
      481 tgaaaaagaa agaatgcggc gcaccgctat gatatcttaa ttcatgcttt tgcgaaacat
      541 gcaagagcgt atagtagatt aatttttgat attttcatcc tttgttctca ttgccgaaaa
      601 gggacgattc cgctcggatt ccgtcgaaaa atcagcgcgg aaaaatttga cagaacttcc
      661 gaatttgaat ataatctccg tccttgatca cttcacccga ttcgacgaca ccggcaaaaa
      721 catccattga aatttttcgg cgaacacagt cttctccgct ccaggagaaa aagagattca
      781 accatcgctt cagatcgcaa atccatcgtt tcgcttcaag agcccgtcag gagtacgacg
      841 taacatgtcg ctttcatact gtacgatttt ctagctttct ttcggatgcg gcgcacattg
      901 acaatcggaa cgggagatat ccgacggtcc cggaggtatt caccgaaagc ggggatattt
      961 taagggtttc cgcgcttccg aagaccggag aattgcttcc cgggattact ctcaggaggg
     1021 aaaagacgaa tgctcgatat cggtgattac agagagaacg tcgcgctgat gctggatatt
     1081 tcgatcctcg gtccgcttcc ctgcacgctg ttcttcctct ttgcgatgcc ggcggcggag
     1141 atgagcatgc tcttccagaa cacgctctac atgaaggacg tagggtactc gttcgtggcc
     1201 tcgtttctgc gggagcgttt cagcccgttt cttgtgacga ccgaccatct gccgcttctc
     1261 ctcgcccagt actttctcct ggcggggcgc ttcctgccgg ctttcgggct gatagccttc
     1321 gccaacttac agatctcgct ccgccaaagc atcctccact cgctggaaac ggaggggaac
     1381 gaaaaagacg ggagcgcggt ccgagcggtc aacggtcttt tgaacctgct ctttttgctc
     1441 cctctgacgg cctttcttct gaaggccctc ggagcggcgt agacaagata aaccgcgaac
     1501 agacgaaagg aaaggacgag agaagatgat cgaacgggaa ttcggcatgg aggatgtgct
     1561 cagcttcttg acgggcattc tgatgaggga gaatctctcg gatctcgtgg agatagcctc
     1621 cttcgttctg gagcggaagg tctctctcaa agagcttcca ttcatcgccg acgagatacg
     1681 gaggcgcatt ctgaagcagc atccgtggct gcgctctctc gaagaggagg atggggagtg
     1741 cgtggagata gccgaatggg tgcgatcggt cgaggcgaaa tacggcggga cgatcctctt
     1801 gaaggccgag gatttctcga agaagaaccg gttccggagg ctgcgggaga gagcggcgaa
     1861 acttctcttc aagacggaga ggctgggttt ttaggggatc aggcatccac ggaggagagg
     1921 gtgcggcaga catcgcgcga aacgctctcg aagcccggcg tgaaggaagg cgatatttta
     1981 caggacgggt cgctgcgctc cccttcgtct tacgttgtcg ctctcctgca gcagcatcgc
     2041 ctccgtatct tcttttcccc cgttttcaat tagagatggg gaggagtttc attgggaaag
     2101 aaccattcgg agcgcggcga aaccggaccg cgaaggagga gaagaaagga gcgcacggat
     2161 gcgcgaaaaa aaagccggat gcacagcgga ggaggagaac gagccggaga cgtacatgct
     2221 ccgggagggc gtcggtctgt tcgtcgacta ctttcgaaac agcttccccg atcttgagga
     2281 ataccgcgtg cgataccttc ccgtgatcga aatgctcgcc gcatcaaccc cgccccttcc
     2341 ggagcgcgct gtcggggaga ttctccaccg gggacgcacg gagacgcgtg ctcttctgaa
     2401 cgaactcggg ccg
//
LOCUS       DAOSWW010000378         2409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34590_length_2409_cov_3.443500, whole genome shotgun sequence.
ACCESSION   DAOSWW010000378 DAOSWW010000000
VERSION     DAOSWW010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34590_length_2409_cov_3.443500"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1202)
                     /locus_tag="PK442_08075"
     CDS             complement(165..1202)
                     /locus_tag="PK442_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOO87645.1"
                     /translation="MKKFALMFLVACLVLTSAAAFAETKTIKLAHLNPQQPFEVATAA
                     MTAVFKSMVEAESNGALKVDIFPAGQLGNERETMEQVKLGVVQTYIASAGGMAAFYPL
                     YSIVDIPFAIPNYDIAWKVYDGPFGQHLAKEIEAKAGFKVLGYGEAGGFFQLSNNTRA
                     IKTVADMKGLKMRTMTLPSHQNLMKAYGAAATPVAWAEVYTALQTGVVDGQHNPIPIV
                     LTGKLFEVQKYLTITNHLYSSYCWVMNKDFYEKLDEKERGIVDAAARTAIIAGRGLNR
                     IIESSEKGLPALIEAGMEINTPTPEALQEFREVGLASSMEFIKETYKDEGVALAQMYL
                     DAIEQAVKEGK"
     gene            complement(1401..>2409)
                     /locus_tag="PK442_08080"
     CDS             complement(1401..>2409)
                     /locus_tag="PK442_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HOO87646.1"
                     /translation="ESIAILPEGMSRERFEWLETVAGEIIATPGTESNVKEIYDKVWE
                     LRRTRDNIVVFNQFDEMGNHLWHYEVTGHAMEEVLREVLGADGRYAGVVVTSGSAGTT
                     ACGDYLKEVFPNSKVGVGEALQCPTLLENGFGAHRIEGIGDKHVPWVHNVKNTDLVMA
                     IDDDDSMGFIRLCNEPAGRKYLRSLGLKDDFIDQLPLMGISGAANVLMAVKMAKYYEL
                     TSKDVILTVFTDSMEMYGSRLKEMEEEFGAYTEMNAAADHARHVLGVRTDGLLELTYQ
                     DRKRVHNLKYYTWVEQQGKTSEELNAQWYDAEEYWGAIHAMADKIDEKIEEFNKMTGL
                     L"
BASE COUNT          437 a          753 c          665 g          554 t
ORIGIN      
        1 gacggtgacg gtcattacta tggagaggag aaagaccggg atctccaccg cgcgctccag
       61 cccgtcgccc actttacggg caatgagaaa gaggcctctc tcctctggcg ttttcgtact
      121 cttcccgtcc ataaaactcg gggagggaga atagggcttg cgccctattt cccctccttt
      181 actgcctgtt cgatggcgtc gaggtacatc tgtgcgagcg cgacgccttc atccttataa
      241 gtctccttga taaactccat cgaagaagca agacctacct cccggaactc ctgcagcgcc
      301 tcgggagtgg gagtgttgat ctccatcccg gcctcgataa gcgcgggaag ccccttctcg
      361 gaagactcga tgatccggtt caggcctcgt ccggcgatga tcgcggtccg cgccgccgca
      421 tcgacgatgc cccgttcctt ctcatccagc ttttcgtaga agtccttgtt catcacccag
      481 cagtagctgc tgtagaggtg gttggtgatg gtgaggtact tctgaacttc aaagagcttt
      541 ccggtgagca cgatgggaat ggggttgtgc tgtccgtcga cgacgcccgt ctgcagcgcg
      601 gtgtacactt cagcccacgc aacgggggta gccgcggcgc cgtaggcctt catgaggttc
      661 tggtgggaag gaagcgtcat tgtccgcatc ttgaggccct tcatatccgc cacagtcttg
      721 atcgcccttg tgttgttcga gagctggaag aagccgcccg cctcgccgta gccgagaact
      781 ttgaatccag ccttcgcctc tatctctttc gcgaggtgct ggccgaaggg accgtcatat
      841 accttccatg cgatgtcata gttgggaatg gcgaagggga tgtcgacgat gctgtaaagg
      901 gggtagaacg cggccatgcc gcccgccgag gcgatgtagg tctggacaac gccgagcttc
      961 acctgctcca tcgtctcgcg ttcgttgccg agctgccctg caggaaagat gtcgacctta
     1021 agagcgccgt tgctctctgc ctcgaccatg ctcttaaata cggcggtcat cgccgccgtc
     1081 gccacctcga acggctgctg gggattcaga tgggccagct tgatcgtctt ggtctcggcg
     1141 aacgccgccg cggatgtcag cacaaggcac gctacaagga acatcaatgc aaatttcttc
     1201 attttgtctc ctccttctca gtcaacgact cccgtagaaa caacgggaac tctctgaact
     1261 ccgtcctcca ttcccttccg ggaaacggaa ggacgctgcg catccggcga cctttctcag
     1321 aacgttttcg gccgcgggac tctccttctc tttgaagcgg catcacctcc cctaagtagt
     1381 tcttggggcg gacatgctcg ttacagcaat ccggtcatct tgttgaattc ttcgattttt
     1441 tcgtcgatct tgtccgccat cgcgtgaata gctccccagt actcctccgc gtcgtaccac
     1501 tgcgcgttca gttcctcgga ggtctttccc tgctgctcta cccatgtgta gtacttcagg
     1561 ttatgcacac gtttcctgtc ttgatacgtc agctccagaa gaccgtccgt ccgcacgccg
     1621 agcacgtggc gagcgtgatc cgcagccgcg ttcatctccg tataggcgcc gaactcctcc
     1681 tccatctcct tcagacgaga accgtacatc tccatcgagt cggtgaagac tgtcaggatg
     1741 acgtccttcg acgtcagttc gtagtacttc gccatcttga ccgccatcag cacgttcgcc
     1801 gcgccggaga tgcccatcag cgggagctgg tcgatgaagt cgtccttcag tccgagcgaa
     1861 cgcaggtact tgcgaccggc aggttcgttg cacagacgga tgaagcccat gctgtcgtcg
     1921 tcgtcgatgg ccataaccaa gtctgtattc ttgacgttat gcacccaggg gacgtgcttg
     1981 tcgccgatgc cctcgatcct gtgtgcgccg aagccgttct caagcagcgt ggggcactgc
     2041 agcgcctcgc cgacgccgac cttgctgttc gggaagactt ccttgaggta atcgccgcag
     2101 gcggtcgttc ccgccgaacc ggaggtcacc acgacgcccg cgtagcggcc gtccgcgccc
     2161 aggacctcgc ggaggacctc ctccatggca tgtccggtga cttcgtaatg ccagaggtgg
     2221 ttccccatct cgtcgaactg gttgaagacg acgatgttat cccgcgtgcg gcggagctcc
     2281 cagaccttgt cgtaaatctc cttgacgttg ctctccgtcc ccggagtagc gatgatctca
     2341 cccgccaccg tctcgagcca ctcaaagcgc tcgcggctca tgccctcggg aaggatggcg
     2401 atggattcg
//
LOCUS       DAOSWW010000379         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34765_length_2401_cov_3.419437, whole genome shotgun sequence.
ACCESSION   DAOSWW010000379 DAOSWW010000000
VERSION     DAOSWW010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34765_length_2401_cov_3.419437"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>2401
                     /locus_tag="PK442_08085"
     CDS             20..>2401
                     /locus_tag="PK442_08085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO87647.1"
                     /translation="MSDTYARYAALDLSSFDGVVSTTIPGCAAVHPNKVCHLMRTARR
                     FYDMFDTEHPSPSPEHRQLRRHLQELDRAAFKDLDTVFVASGEIRGRLLKYLGVDSEV
                     LRPSTTLGGLHTASYEFLLLPGRLHKWKRVDLVVKAMAYVKSQVELLICGEGEEDAAL
                     KALAAGDARIHFLGRVSDEKLTVFYSRALAVPYVPVGEDFGFVALEAALCEKPVITCV
                     DSGEPASMVYDGRTGFVCAADPKEIGRCIDSLAQHTDIASAMGRIGRGSVGHVSWEHV
                     ATVLVRSLGFAGTSAISSPVLAGGGIPSPRPLVLDAQPLLPAIDGGRARALGLYGNAG
                     YDVRYLGVLEEGGEKRNLRLTPTLEELDIPLSQEHLAERDALRKQPGGILALDVSFPL
                     LGHLTPEYAATAERMLSEANAVIFSRPWCYPLVSRALAEKMILTVYDAHNVEGVMRCA
                     LMDDGASGTELVGFVGELESSLCSRADLILAPSHRDRLSLARLYEIPHERIRVVPNGA
                     FTERVTPASEERKITLKEAEGLTDAEKEIPVAVFMGSASQANVEAAEFILREVAPAMP
                     HVVFLIFGSASEAVACTAPQNARLLGVVDEERKRDLLSMADVALNPLFSDCGGAAKMF
                     EYMAAGLPVLATPPAVRGIDLGGTEVVAFSSRERFVDDLRVLLEEPTELARSGAAGRC
                     VAEERHSWEPISRDLGMLIAWHASHRRRRRPFFSVVVPGYERPKLLSRMARLLSVQTY
                     TDFEVLFIDQSRTPWNESGAYRSLDLLYHRIDVKGAARARNIGAFLSRGKVLVF"
BASE COUNT          444 a          703 c          720 g          534 t
ORIGIN      
        1 tgaatcatca tatgaggctt tgtcggatac gtatgcgcgg tatgctgcgc ttgatctttc
       61 ctcttttgac ggcgtcgtca gcaccacgat tcccggatgc gccgccgtcc acccgaacaa
      121 ggtctgtcac cttatgcgaa cggcacgacg tttttacgac atgttcgata cggaacaccc
      181 ttccccctcc ccggagcaca gacagttgcg ccgccatctg caggaactgg atcgtgccgc
      241 gttcaaggat ctggacactg ttttcgtcgc aagcggcgag attcggggac gactgctgaa
      301 gtatctcggc gtcgattcgg aggtcctccg tccatcgact accctcggag gtttgcatac
      361 cgcgtcgtac gaattccttt tgcttccggg tcgcctccat aaatggaagc gggttgatct
      421 ggttgtcaag gcaatggcgt atgtcaaaag ccaggttgag cttcttatct gcggcgaagg
      481 agaagaagat gcggctctaa aagctcttgc ggccggagat gcccgtattc acttcctcgg
      541 gagagtgagc gacgaaaaac tgacggtgtt ctactcgcgc gcgcttgcgg tgccgtatgt
      601 gccggtcgga gaggatttcg gttttgtcgc ccttgaagca gccctctgtg aaaaacctgt
      661 gatcacatgc gtggattccg gagagcctgc gagcatggtg tacgatgggc gaaccggctt
      721 tgtctgcgcg gccgatccga aggagatcgg gcggtgtatc gattccttgg cacagcacac
      781 ggacatagcc tctgccatgg ggcgtatcgg gcgcggttcg gtaggacatg tctcgtggga
      841 acacgtcgcc acggtgctcg tgcgttcgct gggttttgcc ggaacgagcg cgatttcctc
      901 gcccgtcttg gccggcggag gaataccttc tccgcgaccc ctcgttctag acgcccagcc
      961 cctactcccg gctatcgacg gcggacgcgc cagagcgctc ggtctctatg gaaacgccgg
     1021 atatgacgtc cgctacctcg gagttcttga ggagggcggt gagaagcgaa atcttcgcct
     1081 cacgccgaca ctggaagaat tggacatccc tctctctcag gaacatcttg ccgagagaga
     1141 cgcgctgcgg aaacagccgg ggggcatcct cgcgctcgac gtctcctttc cacttcttgg
     1201 gcatctcaca cccgagtacg cagcaactgc tgagcgcatg ctctcggaag caaacgcggt
     1261 gattttttcc cgtccgtggt gctatcctct tgtatccaga gcgcttgcgg aaaaaatgat
     1321 cctgaccgtc tacgacgcgc acaacgtgga gggcgtgatg cgctgcgccc tcatggacga
     1381 cggagcctcg ggtaccgagc tggtcggctt cgtcggcgag cttgagtcga gcctctgttc
     1441 gagggcggat ttgattctcg ctccatctca tcgagaccgg ctttctttgg cccggctgta
     1501 cgaaatcccc catgagcgaa tacgagtcgt tccgaacggc gcctttactg agcgcgtgac
     1561 gccggcgagc gaggagcgaa aaatcacgtt gaaagaggcg gaggggctga cagacgcgga
     1621 gaaagagatt ccggtcgcgg tcttcatggg aagcgcctct caggcgaatg tcgaggctgc
     1681 ggagttcata ctccgcgaag ttgctcccgc aatgccgcac gtcgtcttcc tgatcttcgg
     1741 gagcgcttcc gaagctgttg catgcacggc tccgcagaac gcccgtcttc ttggcgtcgt
     1801 ggacgaagaa cgaaaaagag acctcctgtc gatggcggat gttgcgctca atccgctctt
     1861 ttccgactgc gggggcgccg caaaaatgtt tgagtatatg gcagccggtc ttcccgtgct
     1921 tgcaacgcct cccgccgtcc gcgggataga tctcggcggt acggaagtcg ttgcgttctc
     1981 ctcccgcgaa cgcttcgtcg acgatctgcg cgtcctgctc gaagaaccca ccgagcttgc
     2041 gcggagcggc gcagccggac ggtgcgtcgc cgaagaacgg cactcctggg agccgatctc
     2101 ccgcgacctg ggaatgttga ttgcatggca cgcttcgcat cgcaggcgga gacgcccgtt
     2161 tttttccgtg gtcgttcccg ggtatgaacg tccaaagttg ctttcgcgca tggctcgcct
     2221 gctctctgtt cagacctaca ccgatttcga ggtgctcttc atcgaccaga gccgtacccc
     2281 atggaacgaa tcaggagcgt atcgttctct tgatctgctc tatcacagaa tagacgttaa
     2341 gggggcggcg cgtgcccgca acatcggcgc ctttctctca cgaggaaagg tgcttgtctt
     2401 t
//
LOCUS       DAOSWW010000380         2398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34821_length_2398_cov_3.927017, whole genome shotgun sequence.
ACCESSION   DAOSWW010000380 DAOSWW010000000
VERSION     DAOSWW010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34821_length_2398_cov_3.927017"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PK442_08090"
     CDS             complement(<1..512)
                     /locus_tag="PK442_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO87648.1"
                     /translation="MKKLLALLLCIMLLLPATSALAVNEELSGKVVIYTSMYQFVIDM
                     MDEAMTKEFPNLDVEFFYGGTGSLQQKLAGEIETGKLGCDMMLVAEPAYSLELKEGGW
                     LHPYVIKDAENLLRFPYDKEGYWYPVRVCNMVLAYNPERYKPEDLPKSFEEFAHDKSL
                     KGLISMGNPLT"
     gene            complement(639..1460)
                     /locus_tag="PK442_08095"
     CDS             complement(639..1460)
                     /locus_tag="PK442_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOO87649.1"
                     /translation="MTHPLSDTLRHKKLYLLDMDGTLYLGDRLFPVALPFLDALRSAG
                     ARYLFLTNNSSRGTGSYVQKLGGMGISACEEDFFTSTDAACAYLRAHYSGAKIYVLGT
                     ASFRRHLKQKGFPVTDQLEDDVDCLLMGYDTELTYQKLIDASILLGRNVTYLATNPDW
                     VCPTAFGYVPDCGSIAQALEHATGRLPLFLGKPQPEIALLAMERAGVSPEDTVLVGDR
                     IYTDIACGKRAGVTSILVFSGETTREVLQESDIQPDFALKDVGEITAVLHALSGR"
     gene            1687..>2398
                     /locus_tag="PK442_08100"
     CDS             1687..>2398
                     /locus_tag="PK442_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87650.1"
                     /translation="MLEQLIAVLDLATLEIALGATSLISCATMLALWRTNSSEKGPAC
                     WAAATFLGGSGLFAHFFLLNFIGGAETLRVTLPFVVGSFPLALLLALEGTLRFQGIGG
                     ERKRAYVLPVFAVALLCTSFLGAENASARYMINDPIMILILLLIAFFLLKRTEGLERG
                     IHAIPSFCCIFLAATLAYRCFFAFSGTTGSEIHSSSRLVYFAFLLWAPGWTCGLCMAV
                     MFRARRFILKIADHDSLTG"
BASE COUNT          519 a          656 c          676 g          547 t
ORIGIN      
        1 gttaaggggt tgcccatgga gataaggccc ttaaggcttt tgtcgtgggc gaactcttca
       61 aagctcttgg gcagatcttc gggcttgtac cgctcgggat tgtacgccaa taccatgttg
      121 cagacgcgaa caggatacca gtagccctcc ttgtcgtagg ggaagcgcaa caggttttcg
      181 gcatccttga tgacgtaggg gtgcagccag ccgccctctt tgagctccag cgagtacgca
      241 ggctcggcca ccagcatcat gtcgcagccc aacttgcctg tttcgatttc gccggccagc
      301 ttctgctgca gggaacctgt gccaccgtag aagaactcta cgtccaggtt tggaaattcc
      361 ttggtcatgg cctcgtccat catatcgatg acgaactggt acatcgaggt gtagatgacg
      421 accttaccgg acagttcttc gtttacagcc agggcgcttg ttgcaggaag cagtaacatg
      481 atgcacagaa gcaacgcaag tagctttttc atgatgttga tgagaacctc cttacgctga
      541 tttttggggc gcatgtccca tatgttgtta tcacattacc atgaacagga aattaccgca
      601 agaagaaaag aaagaaaaaa catgaaaaat acagctgttc aacgaccgga aagcgcatgc
      661 agcacggcgg tgatttcacc gacatccttg agggcaaaat cgggctggat gtcgctttct
      721 tgcaggacct cccttgtggt ttcgccgctg aacaccagga tggaggtgac gccggcgcgt
      781 tttccgcagg cgatgtcggt gtagatgcgg tcgcccacca gcacggtgtc ttcgggtgaa
      841 accccggctc tttccatggc cagcagggcg atttcgggct gcggtttgcc caggaagagg
      901 ggcaaccttc cggtggcgtg ctccagcgcc tgcgcaatgg agccgcagtc cggcacgtag
      961 ccgaaggcgg tggggcatac ccagtcgggg ttggtcgcca ggtaggttac attcctgccg
     1021 agtaagatgc tggcgtctat cagcttctgg tacgtaagct ctgtatcgta gcccatcagg
     1081 aggcagtcga cgtcgtcctc cagttgatcg gtgacgggaa agcccttctg cttcaggtgg
     1141 cgccggaagg acgcggtgcc cagaacgtag atcttggcgc cgctgtaatg agcgcgcagg
     1201 taggcgcagg cggcatccgt ggaggtgaaa aaatcctcct cgcaagcgga aatgcccatg
     1261 ccgcctaact tttgcacata gctgccggta ccgcggctgg agttgttggt taaaaagaga
     1321 taccgggcgc ccgccgagcg cagtgcatca agaaagggca gagcgacagg gaacagccgg
     1381 tcgcccaggt acagggtgcc gtccatgtcc aaaaggtaca actttttatg gcgcagggtg
     1441 tcggaaagag gatgtgtcat aatgtctcct ttgtttgctt gtttgtcacg agcgttgaaa
     1501 taaactcata atagcccgaa atacaatggc tggcaaggaa aagaaaggaa agtttatcgc
     1561 aggcgacaaa atagtacaaa aaacccgcgc gaaatcacat caaaagtgat agaattgcga
     1621 cgcattattt tttccacgac ggcgcgcaac ggacattcag acaaatattt gcggaggcga
     1681 agatcaatgc tcgaacaact catcgccgtt ctcgatctgg cgacgctcga aatcgccctc
     1741 ggggcgacct ccctgatctc atgcgcaaca atgctcgcac tctggagaac gaattcctcc
     1801 gaaaaaggcc ccgcctgctg ggcggcggcc actttcctcg gaggttccgg tctgtttgcg
     1861 cattttttcc ttctgaattt cataggaggg gccgaaaccc ttcgcgttac gctccctttc
     1921 gtcgtcggca gctttcccct cgcactgctt ctggcccttg agggaactct ccgttttcaa
     1981 ggtatcggcg gcgaacgcaa acgggcgtat gtactgcctg tgttcgcagt cgcactgtta
     2041 tgtacgtctt ttctgggtgc ggagaacgcc tctgcacgat acatgatcaa cgaccctatc
     2101 atgatcctca tcctgcttct catcgcattc ttcctattga agagaacgga aggactggaa
     2161 cggggcatcc acgcaattcc ttcgttctgc tgcattttcc tcgcggcgac gctggcctac
     2221 cgctgtttct tcgccttctc cgggacgacc ggaagcgaga ttcactcatc ctcccggctc
     2281 gtctacttcg cgttcctcct ctgggcgccg gggtggacct gcggcctctg catggcggtg
     2341 atgttcaggg cgcggcgctt tatcctgaag atcgccgatc acgacagcct gaccggcc
//
LOCUS       DAOSWW010000381         2396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34896_length_2396_cov_2.328492, whole genome shotgun sequence.
ACCESSION   DAOSWW010000381 DAOSWW010000000
VERSION     DAOSWW010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34896_length_2396_cov_2.328492"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PK442_08105"
     CDS             <1..667
                     /locus_tag="PK442_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87651.1"
                     /translation="ARADLDAMVDARGRIASELERMGTDERLGEQLFARKGMERKLDE
                     HLEEWLSNLLAHHFLEVSRARHERERQPEVIRRAGEYLALMTGDRYHLLSEGDEKGFS
                     VVLEEKQQSRERKDELKWSSGLGDQVYLSMRLALASLWGKNSEPLPLILDDLLVRFDE
                     GRQRGAAMAICEAARENQVMLFTCQKGTLDVFRDVVAETGESGSDFLSFHRIENGEFV
                     PVA"
     gene            642..1703
                     /gene="murB"
                     /locus_tag="PK442_08110"
     CDS             642..1703
                     /gene="murB"
                     /locus_tag="PK442_08110"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOO87652.1"
                     /translation="MASLFPLRNASEAPMDAASFPAVATAAAERIRALDVGTVSFREP
                     LKEHCSWEIGGPADIFVQPSEDAQVLRLLRFVREEGLPLVVIGRGTNLLFADEGFRGV
                     VMKFGQRFSDFSVDGASIRARGGLWVPRLVKNLVEAGLSGLEHAAGIPGSFGGLVTMN
                     GGSLRHGVGERVVEVRAVSFAGETRAFSREECGFSYRNSFFQRASDSPESRWIVLEAR
                     LELVPAERNDIRREVLGILEERRGKFPLDKPNCGSVFTNDPSVYELAGPPGKIVEAAG
                     LKGLRVGGAEVSHRHANFIVNLGGATASDVLALVAEIRRRVHDRIGAWLECEVRYVGA
                     DGTLVSASTASECPRPGGK"
     gene            1768..>2396
                     /locus_tag="PK442_08115"
     CDS             1768..>2396
                     /locus_tag="PK442_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87653.1"
                     /translation="MNAFERLSSIFKEFRSLCPKRLFLWTLLLAGIASAWMVSITDKE
                     MRSALLHEARLLAAAINPDRVRSLAGDESDLELESYRRLKTQLGAVRAADPKRRFVYL
                     MGRKRSGEIFIHVDSDPPESVDYSPPGQIYDEATPVHAEVLERGSEFVEGPLEDRWGV
                     WVFALVPLRDSAGVVTALLGMDVGASDWRRNVLKRAALPVALTVALLASL"
BASE COUNT          390 a          734 c          826 g          446 t
ORIGIN      
        1 cgcccgtgca gacctcgacg ccatggtcga cgcccgtggg cgcatcgcct ccgaactgga
       61 gaggatgggg acggacgaac ggctgggcga acagctcttc gccagaaaag ggatggagcg
      121 gaagctcgac gagcaccttg aagagtggct ctcgaacctc ctggcgcatc acttcctgga
      181 agtctccaga gcgcgtcacg agcgcgaacg gcagccggag gtcatccgcc gggcgggcga
      241 gtacctcgcc ctgatgaccg gcgaccgcta ccatctgctc tccgaaggag atgagaaggg
      301 gttctccgtg gtgctggagg agaaacagca gtcccgcgag cggaaggatg agctcaagtg
      361 gagttccggc ctgggcgacc aggtctacct ttcgatgcgg cttgccctcg cctctctgtg
      421 ggggaagaac tccgagccat tgccgctgat tctcgacgac cttctggtgc gcttcgacga
      481 ggggcgccag cgcggcgcgg cgatggcgat ctgcgaggcc gcccgcgaga accaggtgat
      541 gctcttcacc tgtcagaagg ggacgctcga cgtcttccgc gacgtcgtcg ccgagacggg
      601 agagagcggg tcggatttcc tctccttcca ccgcatcgag aatggcgagt ttgttcccgt
      661 tgcgtaacgc ctccgaagcg ccaatggacg cggcttcgtt cccggccgtc gcaacggcgg
      721 cggccgagag gatccgcgcg ctcgacgtgg gaacggtctc cttccgggag ccgctgaagg
      781 agcactgctc gtgggaaatc ggcggcccgg cggatatttt cgtccagcct tcggaggacg
      841 cgcaggtgct ccgcctcctc cgctttgtcc gggaggaggg gctccccctc gtagtgatcg
      901 gcagggggac caatcttctc ttcgccgatg aagggttccg gggcgtggtc atgaagttcg
      961 gacagcgttt ctccgatttc tccgtcgacg gcgcctcgat ccgtgcccgc ggaggcctct
     1021 gggttccgcg tctggtgaaa aatcttgtcg aagccggact ctcgggcctt gagcacgcgg
     1081 ccgggatccc cggctcgttc ggaggcctcg tcacgatgaa cggcggtagt ctccggcacg
     1141 gggtcggcga acgcgtcgtc gaggttcggg cggtctcctt cgcgggggag acgcgcgcct
     1201 tttcgcgcga ggagtgcgga ttctcctaca gaaactcgtt ctttcagcgc gcctcggact
     1261 ccccggagag ccggtggatt gtccttgaag cccgtctcga actcgtcccg gcggagcgga
     1321 acgatatccg cagggaggtg ctgggcatcc tcgaggagcg gcgagggaag tttccgctcg
     1381 acaagccgaa ctgcggctcc gtcttcacga acgatccgtc cgtctacgaa cttgcgggcc
     1441 ctccggggaa gatcgtcgaa gcggccggat tgaaagggtt gcgggtcggc ggcgcggagg
     1501 tctcgcaccg gcacgccaac ttcatcgtca acctcggcgg cgcgaccgcg tcggacgtgc
     1561 tcgctctcgt cgcggagatt cgcaggcgcg ttcacgaccg tatcggcgcg tggctcgagt
     1621 gcgaagtccg ctacgtcggc gccgacggaa cgctggtttc cgcgtcgacg gcctcggagt
     1681 gtccgcggcc cggaggaaag tgaaacgccc cgaggggagc ggtactttgc ggcacgggcg
     1741 ttggtgtacg gaaacggggg gtgcccaatg aacgctttcg aacgcctgag cagcatcttc
     1801 aaggaattcc ggtcgctctg tccgaagcgg ctgttcttgt ggacgctcct cctcgcgggc
     1861 attgcctcgg cgtggatggt gtccatcacc gacaaagaga tgcggagcgc gctccttcat
     1921 gaagcgcgcc tcctggccgc ggcgatcaac cccgatcgag tgcggtcgct cgcgggggac
     1981 gagtccgacc ttgagctgga gtcttaccgc cggttgaaaa cacagttggg cgccgttcgt
     2041 gcggctgatc ccaagcgaag gtttgtttac ctcatgggaa ggaagcgaag cggggagatc
     2101 ttcatccacg tcgacagcga tccgcccgaa tcagtggact actcccctcc cggccagata
     2161 tacgacgaag caaccccggt gcatgccgag gtcctggagc gggggagcga attcgttgaa
     2221 ggccccctgg aagaccggtg gggtgtctgg gtcttcgcgc tggttccttt gcgggactct
     2281 gccggtgttg tgaccgccct tctcggtatg gatgtcggcg cctccgactg gagaaggaat
     2341 gtcctgaagc gggctgcgct cccggtggca cttacggttg ctctcttggc atcgct
//
LOCUS       DAOSWW010000382         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34958_length_2393_cov_4.162532, whole genome shotgun sequence.
ACCESSION   DAOSWW010000382 DAOSWW010000000
VERSION     DAOSWW010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34958_length_2393_cov_4.162532"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PK442_08120"
     CDS             complement(<1..434)
                     /locus_tag="PK442_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO87654.1"
                     /translation="MNESRKILVEKVYAAIKDGSIVKDGKLPTEREMAALMTTSRTSM
                     REALIVLETLGIIEVKGKQGLFVKDAGMGRLNQSLDLYATWPADIIPQIFQVRIMMES
                     PAAGLAARNRTDADLAKMEECLAQFSRIFGESGPDAGREGAQ"
     gene            579..953
                     /locus_tag="PK442_08125"
     CDS             579..953
                     /locus_tag="PK442_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HOO87655.1"
                     /translation="MHKIHLDDVEGTLFPAGRRTKVLVGKDSPLQAKGFVMGRVRVFP
                     GGSIPAHEHFNEEIYHILSGTGEMQVDGETGEVRAGDTVYLDPDVPHTLRNTGDSDME
                     ILFVYSPAGVVEHWREELEGKR"
     gene            985..1764
                     /locus_tag="PK442_08130"
     CDS             985..1764
                     /locus_tag="PK442_08130"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOO87656.1"
                     /translation="MAQKVILVTGSSRGIGLGVLTKHAKLGYAVVMSSSSGEEKATEA
                     LAELRTYGVPADYVKCDISKSADRQAALDFVLKTHGRIDVLVNNAGVAPLTRLNILET
                     TEESFNRLMDINLKGTFFMNQLFANAMISLKKQGLPDYSPRIINIGSISAYTASTNRG
                     EYCISKAGIAMVTALFAAELGAHNIPVFEIRPGITKTDMTKAVEAKYDTFIFEDGGLP
                     TPRWGLPEDIGEAAAALSGGAFDYSTGQVINVDGGFHIRRL"
     gene            1767..>2393
                     /locus_tag="PK442_08135"
     CDS             1767..>2393
                     /locus_tag="PK442_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOO87657.1"
                     /translation="MPVRADREVERGTMRIAGLDLPVHTFNTVVVGTGCAGYNAADTL
                     VSLGQEDVAVVTEGVNMGTSRNTGSDKQTYYKLTLAGGEPDSVRSMAETLFSGGSMHG
                     DIALVEAAMSARGFYKLVNLGVPFPHNAFGEYVGYKTDHDPRQRATSCGPLTSRLMTE
                     HLQKSVEQKGIPVFDGFRVVGILTEGDPRRAIGLVAIDAANYNESTFGL"
BASE COUNT          464 a          817 c          715 g          397 t
ORIGIN      
        1 cactgcgctc cctcgcgccc cgcgtccgga ccgctctcgc cgaagatccg cgagaactgt
       61 gcgagacact cctccatctt cgccaggtcc gcgtccgtcc ggttgcgcgc ggcgagcccc
      121 gcggcagggc tctccatcat gatgcgcacc tggaagatct gggggatgat atccgccggc
      181 cacgtcgcgt acaggtcgag actctggttg agccgcccca tgcccgcgtc cttcacgaag
      241 agcccctgct tccccttcac ctcgatgatc ccgagcgtct ccagcacgat gagcgcctcg
      301 cgcatcgacg tcctgctggt ggtcatcagc gcggccatct cgcgctccgt ggggagcttg
      361 ccgtccttca cgatggagcc gtccttgatc gcggcgtaca ccttctccac cagaattttt
      421 ctcgattcgt tcatttcttc ctccggtttc gactccccgt ctttcggccg agatgctatt
      481 atagagacgc gccaagggga gtatggttcc acttatggtt gtccatggac aaccgcatta
      541 tacacgaaaa acgcttcagt cgaggaggcg atgcccgaat gcacaaaatt catcttgacg
      601 atgtggaggg aacgctcttt cctgcagggc gcaggacgaa ggtgctcgtc gggaaggaca
      661 gcccgcttca ggcgaagggg ttcgtgatgg ggcgcgtccg cgtctttccg ggcggaagca
      721 tccccgcgca cgaacacttc aacgaggaga tctaccacat cctctccgga acaggcgaaa
      781 tgcaggtgga cggcgaaacg ggcgaggtcc gcgccggcga cacggtctac ctggaccccg
      841 acgtccccca cacgctgcgc aacaccggcg actcggacat ggagattctg ttcgtctact
      901 cccccgcggg cgtggtcgaa cactggcggg aagagctgga agggaagcgc tgaaggccct
      961 tcgcacgaaa ggagatacga agacatggca cagaaagtca ttctggtaac gggatcgtcg
     1021 cggggcatcg ggctgggcgt gctcacaaag cacgcgaagc tggggtacgc ggtggtgatg
     1081 agcagctcct ccggcgagga gaaggcgacg gaggcgctcg ccgaattgag aacgtacggc
     1141 gtccccgcgg actacgtgaa gtgcgacatc tcgaaaagcg ccgacaggca ggccgccctc
     1201 gacttcgtgc tgaagacgca cggacgcatc gacgtgctgg tgaacaacgc gggcgtcgcc
     1261 ccgctgacgc gcctgaacat cctggaaacc acggaagaga gcttcaaccg actgatggac
     1321 atcaacctga aggggacctt cttcatgaac cagctcttcg ccaacgcgat gatctccctc
     1381 aaaaagcagg ggctccccga ctactccccc cggatcatca acatcggctc gatatcggcg
     1441 tacacggcct ccacgaaccg gggcgagtac tgcatctcca aggcgggcat cgccatggtg
     1501 acggcgctct tcgctgcgga actgggcgcg cacaatatcc ccgtcttcga aatacgcccc
     1561 ggcatcacca aaaccgacat gacgaaggcc gtcgaggcca agtacgacac gttcatcttc
     1621 gaggacggcg gcctccccac gccgcgctgg ggccttccgg aggacatcgg cgaggcggcg
     1681 gcggccctgt cgggcggcgc gttcgactac tccaccggcc aggtgatcaa cgtggacggc
     1741 ggcttccaca tccggaggct ctagccatgc ccgtccgagc cgatcgcgag gtcgaacgcg
     1801 gcacgatgcg catcgccggg ctggacctgc ccgtccacac cttcaacacc gttgtcgtgg
     1861 gcaccgggtg cgccgggtac aacgccgccg acacgctcgt ttctctggga caggaggacg
     1921 ttgccgtcgt caccgagggc gtgaacatgg gaacgtcgcg caacaccggc tcggacaaac
     1981 agacctacta caagctcacc ctcgccggag gcgagcccga ctccgtccgc agcatggccg
     2041 aaacgctctt ctccggcggc tccatgcacg gcgacatcgc gctcgtcgag gccgccatga
     2101 gcgcgcgcgg cttctacaag ctggtcaacc tcggcgtccc cttcccccac aacgccttcg
     2161 gcgagtacgt cggctacaag accgaccacg acccgcgcca gcgcgccacc tcctgcggcc
     2221 cgctgacctc ccgcctgatg accgagcacc ttcagaagag cgtcgagcag aagggaatcc
     2281 ccgtcttcga cggcttccgc gtcgtcggaa ttcttacgga aggcgacccc aggcgcgcca
     2341 tcggcctcgt cgccatcgac gccgcaaact acaacgaatc cactttcggc ctg
//
LOCUS       DAOSWW010000383         2393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_34960_length_2393_cov_3.775449, whole genome shotgun sequence.
ACCESSION   DAOSWW010000383 DAOSWW010000000
VERSION     DAOSWW010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34960_length_2393_cov_3.775449"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PK442_08140"
     CDS             complement(<1..773)
                     /locus_tag="PK442_08140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO87658.1"
                     /translation="MPDWEKSTLFRAFRHRNFRLFYMGQIVSLSGLWMQRVAMNWLVY
                     RITGSPLLLGTVDFMAQIPVLLFGSFTGAFLEGRNLRKVIMICQTISMLHALTLAILT
                     LSGVVEYWHILILAAVIGTSDSFEVPARQGFTIRLVEDEQDLGNGIALTSTLFNVTRL
                     IGPSVAGMVIALVGEGICFLMNGMAYSATLTALFLMRFKRPLVSESTKKGVLEGMKEG
                     IRYVAGFLPLRNYLSAIALVSFFAFPYMVLLPVFAREILG"
     gene            complement(780..1664)
                     /locus_tag="PK442_08145"
     CDS             complement(780..1664)
                     /locus_tag="PK442_08145"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOO87659.1"
                     /translation="MSLTFSPLSIDMRSDYTPLFDKCSEKTSDYTFVNIWAWSDERKY
                     ELAFARDLFWPRVSSSAPVYWAPVGNWEATDWESVLPELFPDGMTFDRTPEKLAQLWS
                     ERLGDRVTLEEQRSEWEYVYSVEELVALRGNRFHKKKNLLKQFQREYVSEYKSLGRDD
                     VEEILEMQQLWCEWKSCDDIPGLRAENHAISRVLKKWDLLPGLLCGALYVKEYMVAYA
                     VGEPMTDDMVVVHFEKGLAEYKGVYQAINQSFLANSCSGFAWANREQDMGEEGVRKAK
                     ESYNPSHFLKKYRAVWKG"
     gene            complement(1800..>2393)
                     /locus_tag="PK442_08150"
     CDS             complement(1800..>2393)
                     /locus_tag="PK442_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO87660.1"
                     /translation="VVLQGEREMAKDNRILGNFKLEGIRQAPRGLPQIEVAFDVDANG
                     ILNVTAKDKDTAKEQRITINGSTNLDKSEVERMIRDAQENAAEDRKKKELAEARNELE
                     TVVYQLEKALEQAAANVPENEKARARNLVEEGKSALSKSDATLDALRVASSDIKQMLQ
                     ILASSNAGGPEPQPASSARSGDDVVDAEFVDNSENRG"
BASE COUNT          504 a          745 c          645 g          499 t
ORIGIN      
        1 ccgagaatct cgcgggcaaa gacggggagc agcaccatgt acgggaaggc gaagaagctc
       61 acaagagcga tcgcggagag gtagttcctc aggggcagaa agcctgcgac gtaacgaatt
      121 ccctccttca tcccttcgag cacacccttc ttcgtacttt ccgaaacgag cggacgcttg
      181 aagcgcatca ggaagagcgc cgtgagcgtc gccgaatagg ccataccgtt catcaggaag
      241 cagatgccct cccctaccaa tgcgatgacc ataccggcga ccgacggacc gatgagacga
      301 gtgacgttga aaagagtgga cgtgagggcg atgccgttgc cgagatcctg ttcgtcctcc
      361 accaggcgta tcgtaaaccc ctgccgggcc ggaacctcga aagaatccga cgttccgatg
      421 acagcggcga gtatcaggat atgccagtac tcgacaacgc cgcttaacgt cagaatggca
      481 agcgtaaggg cgtggagcat gctgatggtc tgacagatca tgatgacctt ccgcaggttc
      541 cgcccctcca gaaaggcccc cgtaaaagag ccgaagagca gcacgggaat ctgcgccatg
      601 aaatcgaccg tccccaaaag cagcggcgaa cccgtgattc tgtagacaag ccagttcatg
      661 gcaacccgtt gcatccagag tccggagagc gagacgatct gccccatgta gaacaggcgg
      721 aaattccggt gccggaaggc ccggaagagc gtgctcttct cccagtcggg catatcgctc
      781 taccccttcc agacggcgcg gtacttctta aggaagtgcg acgggttgta cgattccttg
      841 gccttccgaa ccccctcctc gcccatgtcc tgctcgcggt tggcccacgc gaagccggaa
      901 caggagttcg ccaggaagga ctggttgatc gcctggtaga cgcccttgta ctccgcaagc
      961 cccttctcga aatgcaccac gaccatgtcg tccgtcatcg gctcgcccac ggcgtaggct
     1021 accatgtact ctttaacgta gagcgcgccg cagagcaacc ctggcaggag atcccacttc
     1081 ttcaagacac gcgaaattgc gtggttctcc gcccgaaggc cggggatgtc gtcgcagctc
     1141 ttccactcgc accaaagctg ctgcatctcg aggatttcct caacatcgtc gcgtccgagc
     1201 gacttatatt cagaaacgta ctccctctga aactgcttca ggaggttctt tttcttgtga
     1261 aaacggtttc cgcgaagcgc cacaagctcc tccacggagt agacgtactc ccattccgaa
     1321 cgctgttcct ccagcgtcac gcggtcgccc agacgttcgc tccaaagctg cgcgagtttc
     1381 tccggcgtcc ggtcgaacgt catgccgtca gggaagagct ccggcagcac actctcccaa
     1441 tcggtcgcct cccagttacc aaccggcgcc cagtacaccg gagcggagct gctcacgcgc
     1501 ggccagaaaa gatccctggc gaaagccaac tcatacttgc gttcatccga ccatgcccag
     1561 atattcacga acgtgtagtc ggatgttttt tcggaacatt tgtcgaaaag aggggtgtag
     1621 tcgctcctca tatctatcga aagcggcgaa aaagtcaatg acatcctgtc acctccagta
     1681 atgaaagtag tatacccgta aagaagcctg cgggcaattt ccccgcaggc ttctctctat
     1741 tgaaaaccgg caaatcgccg gaagtgtgtt ttcaggccgc cggcccttcg ttaaaagcct
     1801 cagccgcggt tttcggagtt atccacaaat tcggcgtcca caacgtcgtc gccgcttcta
     1861 gccgaggatg cgggctgcgg ttcagggcct ccggcgttcg acgacgccag aatttgcagc
     1921 atctgcttga tatcggagga ggcgacgcga agggcgtcca gcgttgcgtc gctcttggac
     1981 aatgcagatt ttccctcctc cacgaggtta cgggcccggg ccttctcgtt ctccggtacg
     2041 ttcgccgccg cctgttcaag cgccttctcc aactggtaga caaccgtttc cagttcattc
     2101 cgggcctcgg cgagctcttt cttcttcctg tcctcggcgg cgttctcctg ggcatcgcgg
     2161 atcatgcgct ccacttcgga tttgtcgaga tttgtcgacc cgttgatggt gatacgctgc
     2221 tcctttgcgg tatccttatc cttcgccgtg acgttcagga tgccgttggc gtcgacatcg
     2281 aaagccactt cgatctgcgg caaccctctg ggggcctgcc gtattccctc aagcttgaag
     2341 ttgccgagga tgcggttatc cttcgccatc tcacgttcgc cctggagcac cac
//
LOCUS       DAOSWW010000384         2384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35172_length_2384_cov_4.134392, whole genome shotgun sequence.
ACCESSION   DAOSWW010000384 DAOSWW010000000
VERSION     DAOSWW010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35172_length_2384_cov_4.134392"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PK442_08155"
     CDS             <1..301
                     /locus_tag="PK442_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HOO87661.1"
                     /translation="TDINNQEGLREQAKMSVSLGFSGKAAIHPSQIAIIHEAFSPDEK
                     EVRKAERIVRGAKEAEERGLGVVAVDGRMVDAPVVAQAVRTLELARLAGMEVEIL"
     gene            298..1866
                     /gene="citF"
                     /locus_tag="PK442_08160"
     CDS             298..1866
                     /gene="citF"
                     /locus_tag="PK442_08160"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOO87662.1"
                     /translation="MKPENLVKNAIGREVPTSVQGLGEFTPYAGPRARLDGKHLWTPT
                     LYPRKIAAPTTNKVAHSLKEAIEKSGLRDGMTISFHHHLRNGDALVGMTLSLLDEMGI
                     RNITLAPSSLGDSHDCVADAVRKGIVTRLNTSGVRGEVGKAISNGEFELPVMIRSHGG
                     RARAVEEGSLHIDVAFLAAPACDRLGNFTGSAGKTACGSMGYAMLDARYADHVIAVTD
                     NLAEYPLAPRISVPQYLVDQVVVVDSIGDPSKIATGATRVTRDPVQLRIAELAFGLLK
                     ASGTLKNGCSFQTGGGGPSLAVARYVKEYMKSHAIKGSYCIGGITGYMASMLEEGLFD
                     ALFDVQSFDAAVTSSIANNPRHIEVDVSCYANPFNKGCLVNDLDVVILAALDVDADFN
                     VDVLTGHDGVLRGASGGHSDTAAGAKLTVITIPSMRNGVPSIRNRVQTVVTPGETVDV
                     IVTERGMAINPLREDLRALATDAGLPVKDIKQLKAEIEKVTGIPEEVRFGDEIVGLVE
                     YRDGTIIDTIRKVL"
     gene            complement(2003..>2384)
                     /locus_tag="PK442_08165"
     CDS             complement(2003..>2384)
                     /locus_tag="PK442_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO87663.1"
                     /translation="RIVNSTSSSVREAIENLILSNTFRLGVIGLTKSLAAEFAPYGIL
                     VNAIGPGRFDTERIRSLDRSLAEKKGLSVEEVSRASLSRIPMGRYGDPEEYGRLAVFL
                     GSEANTYITGQTILADGGMTRAVP"
BASE COUNT          466 a          765 c          766 g          387 t
ORIGIN      
        1 caccgacatc aataaccagg aaggactccg cgaacaggca aagatgtcgg tctctctggg
       61 cttctccggc aaggccgcca ttcacccctc gcagatcgcg atcattcacg aagcgttctc
      121 cccggacgag aaagaggtcc ggaaggccga gcgcatcgtc cggggggcaa aggaagcaga
      181 agagcgcgga ctgggcgtcg tggccgtcga cggccgcatg gtggacgcgc ccgtcgtcgc
      241 gcaggccgtc cggacgctcg aacttgcccg tcttgcggga atggaggtcg agatcctatg
      301 aaaccggaga atcttgtgaa gaacgccatc ggaagagagg tcccgacgtc cgttcagggg
      361 ctcggagagt tcactcccta cgcgggcccg cgcgcacggc tcgacgggaa gcacctctgg
      421 acgccgacgc tctacccgag aaaaatcgcc gcgccgacga cgaacaaggt agcgcacagc
      481 ctcaaggaag cgatcgagaa gtcgggactc cgcgacggca tgacgatctc cttccatcat
      541 cacttgagga acggcgacgc tctcgtcggg atgacactct cgctgctcga cgagatgggc
      601 atacggaaca tcacgctcgc tccgagttcg ctgggcgact cgcacgactg cgtcgccgac
      661 gccgtgcgca agggaatcgt tacgcgcctg aacacttcgg gcgtccgtgg agaggtcggc
      721 aaagcaatat cgaacggcga gttcgagctt cccgtgatga ttcgcagcca cggagggcgt
      781 gcgcgcgccg tcgaggaggg aagcctccac atcgacgtcg ccttcctcgc ggcgccagcc
      841 tgcgacaggc tcggcaactt caccggttcg gcgggaaaga cggcctgcgg ctcgatgggg
      901 tacgcgatgc tcgacgcgcg ctacgcggat catgtgatcg ccgtgaccga caacctcgcg
      961 gagtacccgc tcgcgccgcg catctcggtt ccgcagtacc tggtggatca ggtggtcgtg
     1021 gtcgactcca tcggcgatcc ctcaaagatc gccaccgggg ccacccgcgt aacccgcgac
     1081 cccgtgcagc tcaggatcgc tgagctagcg ttcgggctcc tcaaggcctc ggggacgctg
     1141 aagaacggct gttcattcca gaccggcggc gggggcccaa gcctcgccgt ggcgcgctac
     1201 gtgaaggagt acatgaaatc gcacgcaatc aaagggagct actgcatcgg cggcatcacc
     1261 ggctatatgg cgtccatgct ggaagagggg ctcttcgacg ccctcttcga cgtgcagagc
     1321 ttcgacgcgg ccgtgacctc gtcgatcgcg aacaacccgc ggcacatcga agtggacgtc
     1381 tcctgctacg ccaacccctt caacaaaggg tgccttgtga acgacctcga cgtggtcatt
     1441 ctggccgcgc tcgacgtgga cgcggacttc aacgtcgacg tgctcacagg gcacgacggc
     1501 gtcctcaggg gcgcgtccgg cgggcactcc gatacggccg ccggtgcgaa gctcaccgtg
     1561 atcaccatcc cctcgatgcg caacggcgtg ccctcgatac ggaaccgtgt gcagacggtc
     1621 gtgacgcctg gagagacggt ggacgttatc gtcaccgaac ggggaatggc catcaacccg
     1681 ctccgggagg accttcgggc tctcgccacg gacgcggggc ttcccgtgaa ggacataaag
     1741 cagctgaaag cggagatcga gaaggtcacc ggcatccccg aagaggtccg cttcggcgac
     1801 gagatcgtgg gccttgtgga gtaccgggac ggcacgatca tcgacacgat acggaaagtg
     1861 ctgtagcccg cagaaaaagg tactttctca acaatcaaaa ggccggggga aatcgccgca
     1921 cgcggcgaag gcccctcggc ctttcctgct tctttcaact gaggccccgg ttcggcaaag
     1981 gggactgtct ctgttgccgc ctctacggca ccgccctcgt catgccgccg tcggcgagga
     2041 tcgtctgccc ggtgatgtag gtgttcgcct ccgacccgag gaaaacggcg agccgtccgt
     2101 attcctcggg gtcgccgtac cgtcccatcg gaattcgcga gagcgacgct ctgcttacct
     2161 cctcgacgga cagtcccttc ttctcggcca gcgagcggtc gagcgaccgg atgcgctccg
     2221 tgtcgaagcg ccccgggccg atggcgttca cgaggatgcc gtaaggggcg aactcggcgg
     2281 cgaggctctt cgtcagcccg atcacaccga gccggaacgt attggagaga atgaggttct
     2341 ctatagcctc ccgcaccgaa gaggacgtcg agttgacgat gcgg
//
LOCUS       DAOSWW010000385         2380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35271_length_2380_cov_3.732043, whole genome shotgun sequence.
ACCESSION   DAOSWW010000385 DAOSWW010000000
VERSION     DAOSWW010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35271_length_2380_cov_3.732043"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..709
                     /locus_tag="PK442_08170"
     CDS             335..709
                     /locus_tag="PK442_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO87664.1"
                     /translation="MDEKVGPGGKGSKLALIEEHPILDFVHGEKVRFFFDGKELEGFE
                     GEPISMALHANGVRIYRETAEMHRPRGFFCAIGKCSSCFMIVDGVPNVRTCITPLKAG
                     MKVETQHGKGNVPDLNDGRPRS"
     gene            706..1815
                     /locus_tag="PK442_08175"
     CDS             706..1815
                     /locus_tag="PK442_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO87665.1"
                     /translation="MKKIAPEILVIGGGAAGLSAAAEAASAGAKVLVVESDLRLGGQL
                     IKQTHKFFGSKDEYAGTRGYKIADILLNEIDSFKDSVSILQNTTVTGYYTEDDTFTAM
                     RGEEEVFQIKPQKTIVATGAQERLIPFPNNDLPGVYGAGAVQTLMNVYGVLPGKRVLM
                     VGAGNIGLIVSYQLMQAGVEVAAIVEAGPRIGGYWVHAAKIRRMGVPILLRHSIKKAL
                     GEDVITGAVITGLNEKFEQVGPEEEIECDLICIAVGLSPTTELLSQAGCKMMYVPQLC
                     GNVAQRDRTMRTSNPAFYTAGDASGIEEASAAMVEGRIAGLSAAAALGYPTEHGKIDE
                     YWSRLESLRMGEAGARICQGIECVLVKGWEGASHE"
     gene            1808..2320
                     /locus_tag="PK442_08180"
     CDS             1808..2320
                     /locus_tag="PK442_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO87666.1"
                     /translation="MSDKNMDMLFNSGVLTGEREGAILPPRELWEKKKGGVVIIECPQ
                     RIPCNPCHTSCPTGAILPFEDINNTPKVNWEKCTGCTLCVSACPGLACFVIDLTFSEE
                     NALYKLPYEMLPLPVKGQKVQCLDREGKSVGEGTVENVTQPRKDRTHVVHVSAPKSLV
                     QIFRAIRVVK"
BASE COUNT          648 a          568 c          626 g          538 t
ORIGIN      
        1 tatcgttttt atctattttt actttatcga tataaaagat aaatacatgt tctgcctatt
       61 ttgcctcttt caatagcttc ggaaaaaaat cacgaattga ctcttgacaa agttccacct
      121 cacggaagag aattgaacac agtaaaccct atcctaattc gggaggagat atcaacccga
      181 gctaaaaaca cgttatacgt ccatcgtctt caccgctcta tatttttcca gatttcgcgc
      241 ataacggcgg acacaaaaca gataccaccc tttagggggg tattacgcca tgtgatttgt
      301 gcatgttttt gatctgcgaa aggaggaagc tcttttggat gagaaagtag gcccgggagg
      361 aaaaggaagc aaactcgccc tgatcgagga acatcctatt cttgattttg tccacgggga
      421 aaaggtccgc ttcttttttg acggtaagga gctcgaagga tttgagggag aacctatttc
      481 aatggcgctt catgccaacg gagtgcgaat ctaccgggaa acggcggaaa tgcacaggcc
      541 taggggattt ttctgcgcaa taggtaaatg cagcagttgc ttcatgatcg tcgacggggt
      601 tcccaacgtg cgaacctgca ttacgccact taaagccgga atgaaagtcg aaacccagca
      661 tggtaaaggg aacgttcccg acttaaacga cgggaggccg cgttcatgaa aaaaatagcc
      721 ccagaaatcc tcgtaatcgg tggaggcgcc gcaggactga gcgcagcggc agaagccgca
      781 tcggccggag cgaaggtatt agtcgtcgaa agcgatcttc gcctcggggg ccagctcatc
      841 aaacagactc acaagttctt cggcagtaag gatgaatatg ccggaacccg cggttacaaa
      901 attgcagata tcctgctcaa cgaaatcgat tccttcaaag acagcgtttc tatccttcaa
      961 aacacgacag tcacaggtta ttacaccgaa gacgacacct tcacggccat gcggggcgag
     1021 gaggaagttt ttcagataaa accacaaaaa accattgttg caacaggagc gcaagaacgg
     1081 cttattccct tcccgaacaa cgatcttccc ggcgtttacg gagccggcgc ggtacagact
     1141 ctcatgaatg tttacggtgt tctgcccgga aaacgagtcc tcatggttgg agcaggaaac
     1201 atcggcctta tagttagtta tcaactgatg caggcaggcg ttgaagtcgc cgccatcgtc
     1261 gaagccggtc cgcgtatcgg aggctattgg gtccacgcgg caaaaatacg gcgcatgggc
     1321 gtccctattc tgctgcgcca ttcaataaag aaggcgttgg gagaggacgt tatcaccggc
     1381 gcggttatta cggggctcaa tgaaaaattc gaacaagtgg gccctgaaga agaaatagag
     1441 tgcgacttga tttgcatcgc tgtcggattg agccctacca cagagcttct ttcgcaggcc
     1501 ggatgtaaaa tgatgtacgt tccgcagctt tgcggaaatg ttgcacaaag agaccgcaca
     1561 atgagaacct cgaacccggc tttttatact gccggagacg ccagcggaat cgaagaagcc
     1621 agcgcagcta tggttgaagg acgcattgca ggtctttcag cagcagcagc tcttggttat
     1681 cctaccgagc atggcaaaat cgacgaatac tggagccgtc ttgaatcgct ccgaatgggc
     1741 gaagctggag cccggatatg tcagggtatc gaatgcgttc tcgtaaaagg ctgggagggt
     1801 gcatctcatg agtgacaaaa acatggatat gctattcaac agcggagtct tgaccggaga
     1861 aagagaaggg gccatccttc ctccacgtga actctgggag aagaaaaagg gcggtgtcgt
     1921 gataattgaa tgccctcaga gaatcccctg taatccctgc catacaagct gtcctacggg
     1981 agctattctt ccgtttgagg atatcaataa tactccgaaa gttaactggg aaaagtgcac
     2041 gggttgcact ctctgcgtct cagcctgtcc cggactcgca tgcttcgtta ttgatcttac
     2101 gttctcagag gagaatgcgc tgtacaagct gccgtacgaa atgcttcccc ttccggtgaa
     2161 gggacagaaa gttcagtgcc ttgaccgcga aggtaagtcc gtcggcgagg gaaccgtcga
     2221 gaacgtcact caaccgcgca aggatcgaac gcacgtcgtt catgtctctg cgccgaagag
     2281 cctggtgcaa atattccgtg cgataagggt ggtgaaataa tgagtacttc gcctgagaat
     2341 aatgaaaagg tctatgtctg ccggtgcgag gaagtcacac
//
LOCUS       DAOSWW010000386         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35349_length_2377_cov_3.664944, whole genome shotgun sequence.
ACCESSION   DAOSWW010000386 DAOSWW010000000
VERSION     DAOSWW010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35349_length_2377_cov_3.664944"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PK442_08185"
     CDS             complement(<1..676)
                     /locus_tag="PK442_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87667.1"
                     /translation="METAVAVRAVDSLIEYGRRRRLLGELDDIVARNSLLGLLGIEEP
                     DPTERSGESMDEMASIETLRLYAKERGLAHLDLPAEKFVSRIMGFLTPRNSEVVREFR
                     RLMEQEGAAAAVAWFYRFSADTMYVRMDLVSLDRKWTAMTEFGEMQITINRSKPEKDP
                     RDIRAAGAQTGEDRYPKCLLCAENAGYQGRPGHPERSNHRIVPLELSGEQWYMQFSPY
                     VYYHHHC"
     gene            complement(683..1666)
                     /gene="galE"
                     /locus_tag="PK442_08190"
     CDS             complement(683..1666)
                     /gene="galE"
                     /locus_tag="PK442_08190"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOO87668.1"
                     /translation="MTTSLVCGGAGYIGSHMVRALLERGERVLVLDDLSKGHREYVPD
                     EAVFSKGDIRDGELLDRIFRENAVDAVFHFAAFIQVGESVQRPLEYYENNVTGVLRIL
                     ECMVRHKTPHIVFSSTAAVYGEPDFVPIPEDLPTAPTNPYGETKLAVEKMLRWASAAH
                     GFTYGALRYFNACGAIEDASIGEAHDPETHLIPLVLDAALGRRESISIFGDDYDTPDG
                     TCIRDYVHVTDLAQAHLLTLDALRRGEGNKTFNLGNGRGFSVHEVIDACRRVTGREIR
                     VTLSPRRPGDPARLIASSERIQRELRWKPRYTDLDAIVETAWRWHLKYRKD"
     gene            1905..>2377
                     /locus_tag="PK442_08195"
     CDS             1905..>2377
                     /locus_tag="PK442_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO87669.1"
                     /translation="MKRTVGLAVLIGICGLFLMAGGVFAADLEIGCAIYKFDDTFMTG
                     VRNAIAEAAKGKAKVDIVDSQNSQSTQNDRVDMFITKRMNALAINPVDRTAAGVIIDK
                     AKAENIPVVFFNREPLPEDMQKWDKVYYVGARAEESGTMSGQIIVDFWKSHPEAD"
BASE COUNT          465 a          696 c          739 g          477 t
ORIGIN      
        1 gacagtggtg atggtagtag acgtaggggg agaactgcat gtaccattgt tcgcccgaca
       61 gttcgagcgg gacgatacga tggttgctcc gctcggggtg tccggggcgt ccctggtagc
      121 ccgcattctc ggcgcagagc agacatttcg gataacgatc ctcgcccgtt tgtgctcctg
      181 cggcgcggat atctcttgga tctttctcag gcttggaccg attgatggtg atctgcatct
      241 ctccgaactc cgtcatcgcc gtccacttcc ggtccagcga gacaagatcc atcctgacgt
      301 acatcgtgtc ggcggagaag cggtagaacc aggcgacggc cgccgcggcc ccctcctgct
      361 ccatgaggcg gcggaattca cggacgacct ccgaattgcg gggggtcagg aaccccatga
      421 tacggctgac gaacttttcg gcgggaaggt caagatgcgc aaggccgcgc tctttcgcgt
      481 agaggcgcag cgtttcgatg ctcgccatct cgtccatgct ctcccctgat ctttccgtag
      541 ggtcgggttc ttctatgccg agaagcccga ggagggagtt ccgtgcgaca atatcgtcca
      601 gctcgcccaa gaggcggcgc cgccgcccgt actcgatgag actgtcgacg gcgcggacgg
      661 cgacagcggt ctccacgtcg gctcagtcct ttctgtattt cagatgccac cgccacgcgg
      721 tctcgacgat ggcatccaga tcggtgtagc gcggcttcca gcggagttcc ctctggatac
      781 gctccgagga ggcgatgaga cgggcggggt cgcccggtct cctcggggag agcgtcaccc
      841 ggatctcccg tccggtgacg cggcggcagg cgtcgatgac ttcgtgtacg gagaagccgc
      901 ggccgttgcc gagattgaag gtcttgttcc cctcgcccct tctgagcgcg tcgagggtca
      961 gaagatgcgc ctgcgcgagg tcggtcacgt ggacgtagtc cctgatgcag gtcccgtcgg
     1021 gggtgtcgta gtcgtctccg aagatggaga tgctctcccg tctgcctagc gcagcgtcga
     1081 gaaccagcgg tatcaggtgc gtttccgggt cgtgcgcctc gccgatggac gcgtcctcga
     1141 tggcgccgca ggcgttgaaa tagcgcaggg cgccgtaggt gaatccgtgg gctgcggaag
     1201 cccagcgaag catcttctcg acggcgagct tcgtctcccc gtacggattg gtcggcgccg
     1261 tcggcaggtc ctcggggatc gggacgaaat ccggctctcc gtagaccgca gcggtggaag
     1321 agaaaacaat gtgcggcgtc ttgtggcgta ccatgcactc aagtatgcgc aggacgccgg
     1381 tgacattgtt ctcgtagtat tccagggggc gctgcacgct ctctccgacc tggatgaacg
     1441 ccgcaaagtg gaagacggca tccacggcgt tttccctgaa tatgcggtcg agcagctctc
     1501 cgtcccgaat atcccctttg gagaatacgg cctcgtccgg gacgtattcc cgatgtcctt
     1561 tcgagagatc gtcaagtacg agcacccgct ctccacgctc gagaagcgct ctcaccatat
     1621 gacttccgat atatcctgca ccgccgcaga caagggacgt cgtcacgaat aatcagccct
     1681 ccagccgtga gaaacagtat gaactccgga acagttcgca ccccggattt gggcaacagg
     1741 gtatcccccg accgatgata tgtcaagggc gcgcgttttc agtgtaaatt gaaaatataa
     1801 ttgacaggta ggctcctctt ggctacactt gaccggggga aagaccccac agacgtccgt
     1861 ggagttccat ggcgcgaaat cattgcagag gaggtttttc gaaagtgaag agaacagttg
     1921 gacttgcggt gctcatcggt atttgcgggc tttttcttat ggcgggtggc gtttttgcgg
     1981 cggatcttga gatagggtgc gccatctaca aattcgacga caccttcatg accggcgtca
     2041 gaaacgcgat cgccgaggcg gccaagggca aggcgaaagt cgatatcgtc gacagccaga
     2101 actcccagtc cacccagaac gaccgggtcg acatgttcat caccaagagg atgaatgcgc
     2161 tcgccatcaa cccggtggat cgtaccgcgg ccggcgttat catcgacaag gccaaggccg
     2221 agaacatccc cgtagtcttc ttcaaccgcg aacctcttcc cgaggatatg cagaagtggg
     2281 acaaggtcta ctacgtgggg gcgcgcgcgg aagagtccgg cacaatgtcg gggcagatca
     2341 tcgtcgactt ctggaagagc caccccgaag cggacaa
//
LOCUS       DAOSWW010000387         2368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35560_length_2368_cov_3.336792, whole genome shotgun sequence.
ACCESSION   DAOSWW010000387 DAOSWW010000000
VERSION     DAOSWW010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35560_length_2368_cov_3.336792"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2368)
                     /locus_tag="PK442_08200"
     CDS             complement(<1..>2368)
                     /locus_tag="PK442_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO87670.1"
                     /translation="ANRVRTVTLPEDAEEPVVRKFDINARSFMSVVFTSTLPPQRAFD
                     ILEDRVQRQLSQVVDVADVSLDGGLTREIHVDLDPVALGSLGLSIRQVTNLLKSGNYS
                     SPSGRISLGDQESILRVVGESTTVRELEELQIPLAKGVSVRLADIATVTDATAEVRRL
                     ARYNGRDSFIISLTAVPDGNVVQASRAARAMLDEILPTLPPGFSMAIPFDDGEFIADS
                     VWNVFRDMITGTLLTGLILFLFLRRLSVTLLVGIVMPTAIIATFMPMVLARYTLNMMT
                     TLGLAISTGILVNNSILVIENIIRYRELGYNPLDAAERGTKEIAIAVLSTTATNLGVF
                     IPMALSGGRMGQMFNPFAMTVVFSTLFSLWVALTFTPMLAARIGRSAGPSTLNRFLTG
                     WWQWLYQGFEELHHVLVRGAVRFPLSTILLFCALSAGSLMLAGRIGFQFFPSSDEGQI
                     TISMETSASASLSMTDRFVRQMEQHAISLPYVSGVNAVIGGGGSRSGLNRATMRIYLE
                     DTPDRPSSFAFAAPLRPLLASLPDMKASVTAGGGRGGVRGKPIQVVVRGEEIGALNDL
                     AVKVMEVLRGINGVVDVDLDWRTGRPEIQMIPDRLRMARLNFNSEDLSDMLRGYVTGL
                     KAGVFREGGKEYDILVKLRAEEVSSPFQLPDLPFPTTAGFVSLRDLAALKETMGPTQI
                     LRKDRQRSITVDANVSGVTVGDVYRVIEQRLGEIPLPQGYRFTFAGEIQSIQENFGTL
                     NFALGLAVLLTFLLIAAILESFLFAFVIMLTVPLSVIGVVPALILTQTP"
BASE COUNT          596 a          679 c          658 g          435 t
ORIGIN      
        1 ggcgtctgcg tcagaataag cgccgggaca acgccgataa cggagagcgg aacagtgagc
       61 ataattacga aggcgaagag aaacgactct aagatcgccg caatcaaaag gaaagtaagg
      121 agtaccgcga gcccgagagc gaagttgagg gtaccaaagt tctcttgaat cgactgaatt
      181 tctcccgcga aggtgaaacg gtacccctgt ggaagcggta tctccccgag gcgctgctcg
      241 atgaccctgt atacatcgcc gacggtgact cccgagacgt tcgcatccac cgttattgaa
      301 cgctgtctgt ccttccgcag gatttgcgtc gggcccatcg tctccttcaa cgcggcaaga
      361 tcacggagcg agacgaagcc tgctgtcgta ggaaagggaa ggtctggaag ctggaacgga
      421 ctcgaaacct cctccgcgcg aagcttgacg aggatatcgt actccttgcc accctctcgg
      481 aagaccccgg ccttcagtcc tgtgacgtat ccccggagca tatcggagag atcctccgaa
      541 ttgaaattca gcctcgccat ccggagtctg tcgggaatca tctgaatctc cgggcggcct
      601 gtccgccagt cgagatccac atcaaccaca ccgttaatgc cgcggagaac ctccatgacc
      661 ttcacggcaa ggtcgttcag agcgcctatt tcctctcccc ggacaacaac ttgtataggc
      721 tttccgcgaa cgccgccgcg tccacctccg gcagtgacgc tcgccttcat atccgggaga
      781 gatgccaaaa gaggacggag aggagccgca aaagcaaagc tgctaggcct gtccggcgta
      841 tcctcaagat aaatgcgcat tgtggcacgg ttcagtcctg atctcgaacc gccgccgcca
      901 ataacagcgt tgacaccgga gacataagga agtgaaatgg cgtgttgttc catctgccgg
      961 acaaaacgat ccgtcatcga gagagaagcg gaggccgagg tctccatgct gatggtaatc
     1021 tgcccctcgt cggaggaagg aaaaaattgg aacccgatac gccctgcgag catgagagaa
     1081 ccggcggaaa gagcgcaaaa aagcaggatg gtggagagcg gaaaacgtac ggcgcctcgc
     1141 acaagtacgt ggtgaagctc ttcaaaacct tgatagagcc actgccacca tcccgtcaaa
     1201 aaacggttca aagtcgacgg cccggcagac cttccgatgc gggccgccag catcggcgtg
     1261 aacgtcaagg caacccagag cgagaagagc gtcgaaaaga ccaccgtcat ggcgaacggg
     1321 ttaaacatct gccccatgcg gccgccggaa agcgccatgg ggatgaatac ccccagattt
     1381 gtcgctgtgg tggaaagcac cgcgatcgcg atctccttcg taccgcgctc cgcggcgtcc
     1441 agcgggttgt atcccaattc ccggtacctg atgatgtttt cgataaccag gatagagttg
     1501 ttcacaagaa tgccagtcga gatggcgagc ccgagggtcg tcatcatgtt gagcgtatag
     1561 cgcgcaagca ccatcggcat gaaggtcgca attatggcag tcggcatgac aattcccaca
     1621 agcagcgtca ccgaaaggcg gcgcaaaaag agaaagagaa tcaaccccgt cagtagcgta
     1681 ccggtaatca tatcccggaa gacgttccac accgagtcgg cgatgaattc tccgtcgtcg
     1741 aacggaatag ccattgagaa accaggtggg agcgtcggga ggatctcgtc gagcatcgcc
     1801 cgagcggcgc ggctcgcctg tacaacattg ccatcgggaa cagcagtaag cgagatgatg
     1861 aacgaatccc gtccgttgta tcgtgcaaga cggcggacct ccgcggtggc atcagtgacc
     1921 gtcgcaatgt ccgcaagacg tacggaaaca ccttttgcta aaggaatctg cagctcttcc
     1981 aactctcgca cggttgtcga ttcaccgact acgcgtagaa ttgactcctg atcaccaagc
     2041 gaaatccgcc ccgacggaga gctgtagtta ccgcttttca gaaggttcgt cacctggcgg
     2101 atactcagac cgagactgcc gagcgccaca gggtcaaggt cgacatgaat ttcccttgtc
     2161 agtcctccgt ccagcgacac atcggcaacg tcgacaactt gtgaaagctg tcgttgcacc
     2221 ctgtcttcaa ggatatcgaa cgctctctgc ggcggaagcg tcgacgtaaa aacaaccgac
     2281 atgaacgagc gtgcattgat gtcgaacttc ctgacaaccg gttcttcagc atcctccgga
     2341 agcgtgaccg ttctgacgcg gttcgcaa
//
LOCUS       DAOSWW010000388         2368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35563_length_2368_cov_3.287073, whole genome shotgun sequence.
ACCESSION   DAOSWW010000388 DAOSWW010000000
VERSION     DAOSWW010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35563_length_2368_cov_3.287073"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1284
                     /gene="hutI"
                     /locus_tag="PK442_08205"
     CDS             43..1284
                     /gene="hutI"
                     /locus_tag="PK442_08205"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOO87671.1"
                     /translation="MIVKFFRNARIYTPEGKPPSRGREQGALRVFERGAMLVQDGVLE
                     AVGTEEELLSNPLVKKVDEEIDCEGRCLIPGFVDPHTHMCFAARREEEFSMRLAGAAY
                     LDILKKGGGILSSVRHVRGASEQEIFERTRTLALSALSFGTTTVEMKSGYGLDTESEL
                     KMLSAIRRVGRETPLRVVPTFMGAHAIPEVFKGNGDGYVDLMIGEMLPAVAEQGIARF
                     CDVFCEEGVFSPEQTRKVLVEARKYGLGLKIHADEVHDTGGAALAAELGAVSAEHLLA
                     ASDEGIEALAASGTVAVLLPATAYSLRKPYARARTMIEKGVPVALATDCNPGSSFTES
                     MPFVFGLAVLVMGLTVEEALVATTKNSAHAIGLGAECGTLEPGKNADFLLLDGETPAV
                     LAYHAGVSPVKSVFKKGERVA"
     gene            1284..>2368
                     /locus_tag="PK442_08210"
     CDS             1284..>2368
                     /locus_tag="PK442_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOO87672.1"
                     /translation="MIGERKNRVRLDGMSLTLEDVLRVARDGWGVELDEEAARKVEEA
                     SAAVKAWENSDDVVYGITTGFGDLATVNISRKDRRLLQENLLKSHACGVGEPFSEEIT
                     RAMMLLRINSLIRGFSGISLTTLHQFVAFLNLGITPVVPSQGSVGASGDLCPLSHLAL
                     PLLGLGKVRYKGRVILAAKALQTAGLQPVQLGAKEGLALNNGTASMGAMGVVALMAAE
                     DLAKTADIAAALSIEALHGVPYAFDARTHALRAHRGQGRVAENIRRLIEGSEIIEKHR
                     FGRVQDAYSLRCVPQVHGATRDALEYVRRTLETEINSVTDNPIIFTEDGVAISGGNFH
                     GQPLALAMDFFGIAVAELASISERRQA"
BASE COUNT          419 a          750 c          798 g          401 t
ORIGIN      
        1 gcaggttctg gagctgaagc tcctctccgg cggtgatggt gcatgatcgt caaattcttc
       61 cgcaacgccc gcatctacac cccggaggga aagcctccct ccagggggcg tgagcaaggc
      121 gctctgcgcg tttttgaacg aggggcgatg ctcgttcaag atggagttct cgaagccgtc
      181 ggcacggaag aggagcttct tagcaatccc ctcgtgaaaa aggtcgacga agagatcgac
      241 tgtgaagggc gctgtctgat ccccggcttc gtcgatccgc acacgcacat gtgcttcgcc
      301 gcgcggcggg aagaggagtt ctcaatgcgc ctggcggggg cggcgtacct cgacatactc
      361 aagaagggcg gcggcattct ctcctccgtc cggcatgtac ggggcgcgag cgaacaggag
      421 atcttcgagc gcacgaggac gctcgcgctc tccgcgctct ccttcgggac gaccaccgtc
      481 gagatgaaga gcgggtatgg actcgacacg gagagcgagt tgaaaatgct ctcggcgatt
      541 cgccgcgtcg ggcgcgaaac gcccttgcgc gtcgttccca cctttatggg ggcgcacgcg
      601 attccggagg tcttcaaggg gaacggcgac ggttacgtcg atctcatgat cggagagatg
      661 cttcccgccg tcgctgaaca gggaatcgcc cgcttctgcg acgtcttctg cgaggagggg
      721 gtcttctccc cggagcagac gcggaaagtg ctcgtcgagg cccgaaaata cgggctcggc
      781 ctgaagattc acgccgacga ggtgcacgac accggcgggg ccgcgcttgc cgccgaactc
      841 ggcgccgttt ccgcggaaca tcttctcgcg gcgagcgacg aaggcataga ggccctcgcc
      901 gcgagcggaa cggtcgctgt gctgctcccc gctacggcgt acagcttgcg caagccgtat
      961 gcccgcgccc ggaccatgat cgagaagggg gttcccgtgg cccttgcgac cgactgcaac
     1021 cctggttcct ccttcacgga gtccatgccc ttcgtcttcg gcctggccgt actcgtgatg
     1081 ggactcactg tggaagaggc gcttgtggcg acgacgaaga acagcgcgca cgcgatcggc
     1141 ctcggggccg agtgcgggac gctcgagccg ggaaagaacg ccgacttcct cctcctcgac
     1201 ggagagactc cggccgtcct ggcctaccac gccggggtct cgccggtgaa gtccgtcttc
     1261 aagaaagggg agcgtgtcgc atgatgatcg gtgagcggaa gaacagagta cgtctcgacg
     1321 ggatgtcgct taccctcgag gatgtgctcc gcgtcgcgcg cgacgggtgg ggagtcgaac
     1381 tggacgaaga ggccgcccgg aaggtggagg aggcgtccgc cgccgttaaa gcctgggaga
     1441 actccgacga cgtcgtctac ggcatcacca cgggcttcgg cgacctcgcc acggtgaaca
     1501 tctcccgcaa ggatcgtcgc ctgctgcagg agaatctctt gaagagccac gcctgcggcg
     1561 tcggcgagcc gttctccgag gagatcacgc gggccatgat gctgctgcgg atcaacagtc
     1621 tcatcagagg gttctcgggt atcagtctca cgacgctgca tcagttcgtc gcttttttga
     1681 atctggggat cactcccgtc gttccgtcgc aggggtcggt gggcgccagc ggcgatctct
     1741 gccccctctc gcacctcgcg ctcccgctgc tggggctcgg aaaggtgcgt tacaaggggc
     1801 gcgtcatcct cgcggccaaa gcgctccaga cggcggggct ccagcctgtg cagctcgggg
     1861 cgaaggaggg actcgccctg aacaacggca cggcctcgat gggcgccatg ggcgtagtgg
     1921 cgctgatggc cgccgaggac cttgccaaga ccgcggacat cgcggcggcg ctctccattg
     1981 aagcgcttca cggcgtcccc tacgccttcg acgcgcggac gcacgcgctc cgcgcccaca
     2041 gagggcaggg gcgcgtggcc gagaatatcc gccgactgat cgaggggagc gaaattatag
     2101 agaagcaccg cttcggcagg gtgcaggacg cctactccct ccgctgcgtg ccgcaggttc
     2161 acggcgccac ccgcgacgcg ctggagtacg tgcggaggac gctggagacg gagatcaact
     2221 cggtcaccga caatccgatc atcttcaccg aggacggcgt ggccatcagc ggcggcaact
     2281 tccacggcca gccgctggcc ctggcgatgg acttcttcgg catcgccgtg gcggagcttg
     2341 cgagcatctc cgagcgcagg caggccag
//
LOCUS       DAOSWW010000389         2366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35613_length_2366_cov_3.923410, whole genome shotgun sequence.
ACCESSION   DAOSWW010000389 DAOSWW010000000
VERSION     DAOSWW010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35613_length_2366_cov_3.923410"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PK442_08215"
     CDS             <1..1046
                     /locus_tag="PK442_08215"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO87673.1"
                     /translation="KAVSYNARIIVMDEPTSSLTGHEVSHLFKIIRSLKARGISIIYI
                     SHKMEEILEIADEVTIMRDGHKVGTWPAKELTTDLIISRMVGREIRNRFPPRRNAPAE
                     EPVLEVRGLTSSFPKSFRDVSFTLRRGEILGVGGLVGAQRTEAMEALFGLRSLSGGKI
                     LLGGKEADLSTPIKAKRRGLAFLTEERRATGIFPVLSVEFNLLVAHLGNYVSCMRLLE
                     QKRMRRDARESIERLAVKTPSSRSLIRDLSGGNQQKVLFARWLLINPEILILDEPTRG
                     IDVGAKYEIYSIMSELASQGKSIIMISSEMPELLGMTDRILVMSQGRLAGIVETKETS
                     EEDIMRLATKYVA"
     gene            1078..2088
                     /gene="mglC"
                     /locus_tag="PK442_08220"
     CDS             1078..2088
                     /gene="mglC"
                     /locus_tag="PK442_08220"
                     /inference="COORDINATES: protein motif:HMM:NF007014.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HOO87674.1"
                     /translation="MESKKTVLGSSVHFLSQNAIFVVLVVLVLLIAVVDPRFLSFAIL
                     RDVLMQSSTRAIMALGVAFILITGGVDLSSGRMVGFAAVIAASMLQIPEYARRFYPDL
                     PHLPVFLPIILAVAACMLFGMLNGLIVAKLQVPPFITTLGTMVVVYGINSIYFDMPPN
                     NSQPIGGLRPDFTALGSGRLMNIPVIILIALAVAVIVWILFNKTQLGMNMYAIGGNRD
                     AAIVSGVNVVLTIVILYAIAGALYGVAGVLEAARTGGATNNYGNMYELDAIAACVVGG
                     VSTTGGVGTVPGVLAGVLIFGVINYGLTFIGVNPYWQLIVKGLIIVAAVSFDIRKYIA
                     KK"
     gene            2109..>2366
                     /locus_tag="PK442_08225"
     CDS             2109..>2366
                     /locus_tag="PK442_08225"
                     /inference="COORDINATES: protein motif:HMM:NF021988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOO87675.1"
                     /translation="MDTRGILTLFEKIYGSSGGAESFFAPGRVNLIGEHTDYNGGHVL
                     PCALTFGTYVAARKRTDGTVRCFSENFPESGVVFFELDEIAH"
BASE COUNT          458 a          666 c          706 g          536 t
ORIGIN      
        1 cgaaagcggt ctcgtacaac gcgcgtatca tcgtcatgga cgaaccgacc tcctcgctca
       61 ccgggcacga ggtcagccac ctgttcaaga ttatccgcag cctgaaggcg cgcggaatct
      121 ccattatcta catttcgcac aagatggagg agattcttga aatagccgac gaagtcacca
      181 tcatgaggga tggacataag gtcgggacct ggccggcgaa ggaactgacg accgatctca
      241 tcatatcccg gatggtcggg cgtgagataa ggaatcgctt ccctccgagg cggaacgcac
      301 ctgcggaaga gcctgttctt gaagtgcgcg gcctgacgtc ttccttcccg aagtcgttcc
      361 gggacgtctc cttcacgctg cgccgggggg agatcctcgg cgtgggaggg ctcgtcggcg
      421 cgcagcgcac cgaagccatg gaggcgctct tcggtctgcg ctctctctcg gggggaaaga
      481 ttctccttgg cggcaaggaa gcggaccttt caacgccgat aaaggcgaag cggcgcgggc
      541 tggccttcct taccgaagag cgccgggcca ccggtatctt tccggtcctc tcggtggagt
      601 tcaaccttct tgtggcgcac ctcggaaact atgtaagctg catgcggctg ctcgaacaga
      661 aacggatgcg cagggatgcc cgtgagagta tcgaacggct tgccgtcaag acgccttcgt
      721 cccggagcct gatccgcgac ctttccggcg gcaatcagca aaaggtgctc tttgcccgct
      781 ggcttctgat caaccccgag attctcatcc tcgacgaacc gacgcgggga atcgacgtcg
      841 gcgcgaagta cgagatttac tcgatcatgt cggagcttgc atcccagggg aagagcatca
      901 tcatgatctc ctccgagatg ccggagcttc tcggcatgac ggacagaatc ctcgtgatga
      961 gccaggggag gcttgcggga atcgtggaaa cgaaggagac ttccgaagag gatatcatgc
     1021 ggcttgccac gaagtacgtg gcgtagcttg agctgacgag ggaggaagga cattcctatg
     1081 gagtcgaaaa agactgttct tggatcttca gtgcattttc tttcacagaa cgccatcttc
     1141 gttgttctgg tcgtgctggt gttacttatc gccgtggtgg acccgcgttt tctctcattc
     1201 gctattttgc gggacgtcct tatgcagagc tccacgcggg cgatcatggc ccttggcgtg
     1261 gcgtttattc ttattaccgg aggcgttgat ctctcctccg ggcgtatggt gggcttcgcg
     1321 gcggtcatcg ccgcctcgat gcttcagata cccgagtatg cgcggcgctt ctaccccgat
     1381 cttccgcatt tgcctgtttt tctcccgatt attcttgcag tggccgcctg catgctcttc
     1441 ggaatgctca acggactgat tgtggcgaag ctccaagtgc ctccgttcat tacgacgctc
     1501 ggtacgatgg tcgtcgtcta cggaatcaat tcgatctatt tcgacatgcc tccgaataat
     1561 tcgcagccta taggcggcct cagaccggat tttaccgcgc tcggttccgg ccggctcatg
     1621 aacatcccgg tgatcattct catagcgctt gcggtcgctg tgatcgtctg gattcttttc
     1681 aacaagacgc agctgggtat gaacatgtac gcgatcggcg gaaacagaga cgccgcgatc
     1741 gtctccggcg tcaacgtcgt cctcacgatc gtcattctct acgccatcgc gggcgcgctg
     1801 tacggcgtgg cgggcgtcct ggaagcggcc cgtaccgggg gcgccaccaa caactacggg
     1861 aacatgtacg aacttgatgc gattgccgca tgcgtcgttg gaggcgtttc gacgacgggg
     1921 ggcgtcggga cggtacccgg cgtgctcgcg ggcgtgctga tcttcggcgt catcaactac
     1981 gggctgacct tcatcggcgt gaatccgtac tggcagctca tcgtcaaggg gctcatcatt
     2041 gtggcggcgg tttccttcga cataagaaaa tacattgcga agaagtgacg gctacgtgcg
     2101 gagtacttat ggatacacgc ggcatcctga cgctttttga aaagatctac ggctcttccg
     2161 ggggggcgga gtccttcttt gcccccggaa gggtgaacct tatcggagaa catacggact
     2221 acaacggggg acacgtactt ccgtgcgccc tgaccttcgg tacgtacgtt gcggccagaa
     2281 aaaggaccga cggcacggtg cgctgtttct ccgagaactt tccggaatcc ggggttgtct
     2341 ttttcgagtt ggatgagata gctcac
//
LOCUS       DAOSWW010000390         2365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35638_length_2365_cov_3.497403, whole genome shotgun sequence.
ACCESSION   DAOSWW010000390 DAOSWW010000000
VERSION     DAOSWW010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35638_length_2365_cov_3.497403"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1044
                     /gene="hrcA"
                     /locus_tag="PK442_08230"
     CDS             28..1044
                     /gene="hrcA"
                     /locus_tag="PK442_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOO87676.1"
                     /translation="MLTERQLEVVFSVVYEYIQTGEPAGSRTISKKYLRGCSPATIRN
                     EMADLEEMGYFSQPHTSAGRLPTSKAYRLYVDAIMQRRRTAPAETEKWKREIGERKQG
                     IESILTYVSRLLGQATNYVGVAALSALEEVQIQRVSCLRLGGNTVLLLIVLEGGLVHH
                     SNIQLPCELSQDMLDELGRRITTIAEGHSWSEVRDVLFRYVLDGLERVWDTCREAIVQ
                     MDALLNRRSSRLFIGGAQHILSLPDFQDIGKFQTVLSLLEQEQALAEMVERYSVKQGV
                     SVTIGDENTEEDMKQLSLVLVPASGQSRRAILGLIGPLRMDYEKSIAILETLAEDLDD
                     MLVQ"
     gene            1069..1674
                     /locus_tag="PK442_08235"
     CDS             1069..1674
                     /locus_tag="PK442_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOO87677.1"
                     /translation="MSSVNDQVSSELEKEGVASASTEESVSRDEPEARQEVAPSPEEL
                     LAALQNAEREKIELTEALARARADFFNYRKRVDRDRQKERSMIAEEKALDFLPVLDNL
                     DRALSVSPDIDGKSVLQGVAMVRKQFLAVLESMGVEAVPSIGSVFSPEYHEAVVAVAT
                     DDPEKNGIVMEEIQSGYRTKERVLRPAKVKVASYTLTAEKG"
     gene            1822..>2365
                     /locus_tag="PK442_08240"
     CDS             1822..>2365
                     /locus_tag="PK442_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO87678.1"
                     /translation="MSKVVGIDLGTTNSCIAVQEGDVTTIIPNAEGNRTTPSVVAFTK
                     EGERLVGQLAKRQAIVNGDRTIMSIKREMGTDHRVTVDGKKYTPQEISSMILQKMKRD
                     AEDYLGEPVTKAVITVPAYFTDAQRQATKDAGAIAGLEVLRIINEPTAACLAYGENKQ
                     GEHKILVFDLGGGTFDVSILD"
BASE COUNT          545 a          604 c          726 g          490 t
ORIGIN      
        1 gtgctaacgc aacagggagg tgtcaaaatg cttacggaga gacagctcga agttgttttt
       61 tccgttgtct acgaatatat ccagacgggc gagccggcgg gttccaggac gatttcgaag
      121 aagtatctcc gggggtgcag ccccgcgacg atacgaaacg agatggctga cctggaagag
      181 atggggtatt tttcccagcc gcatacgtcc gcgggaaggc tgccgacatc gaaggcgtat
      241 cgtttgtatg tggacgcgat catgcagcga agacgtaccg ctcccgccga gacggagaag
      301 tggaagcggg agatcggaga acgaaaacag gggatcgaat cgatcctcac ctatgtttcc
      361 cggctcctcg ggcaggcgac gaactatgtg ggggtagcgg ctctctctgc gcttgaagag
      421 gtacagatcc aacgggtgag ctgccttcgc cttggaggca atactgtgct gcttctgatt
      481 gttcttgaag gcggactggt gcatcactcc aacatccagc ttccctgcga gctttctcag
      541 gacatgcttg acgagcttgg acgccgtatc accaccatag ccgagggcca ttcttggagc
      601 gaagtccgcg atgttctttt cagatatgtt ctcgacggtc tggagcgggt ctgggatacc
      661 tgccgcgagg caatagtcca gatggacgcg ctcttgaaca ggcggagttc ccgcctgttc
      721 atagggggcg cgcagcatat cctgagcctg cccgatttcc aggatatagg gaagtttcag
      781 acggtgctct ctcttcttga acaggaacag gcccttgcag agatggtcga acgctatagt
      841 gtaaaacagg gggtctcggt taccatcgga gatgaaaaca cggaagagga catgaagcag
      901 ctctctcttg ttctcgtgcc ggcatccggc cagagccgca gggcgatcct ggggctgata
      961 ggcccgctcc gtatggacta cgagaagtcg attgccatac tggaaaccct tgctgaagat
     1021 ttggacgata tgctcgtcca gtagttttat atgaaaggag cgacacgaat gagcagtgtt
     1081 aacgatcagg tttcttcgga gctggaaaaa gagggggtcg cctccgcttc aaccgaagag
     1141 agcgtctccc gggacgaacc ggaagctcgt caggaggttg cgccttcacc ggaagagctg
     1201 cttgctgcgc ttcagaatgc ggagcgtgag aaaattgaac tgacggaggc cttggccaga
     1261 gcgcgcgcgg attttttcaa ctatcgcaaa cgggtcgaca gagaccgtca gaaagagcgg
     1321 tcgatgatcg ccgaagagaa ggcgcttgat tttctgccgg tcctcgacaa tctggaccgc
     1381 gcgctttcag tgtccccgga catcgacggg aagagcgtgc ttcaaggtgt cgccatggtc
     1441 aggaaacagt tcctcgcagt tctggaatcg atgggcgtcg aggctgtccc gtctataggc
     1501 tctgtcttct cgccggagta ccacgaagca gtagttgcgg ttgcgactga tgacccggaa
     1561 aaaaacggaa tcgtcatgga ggagatacag tccggttatc ggacgaagga acgcgtgctc
     1621 cgtccggcga aggtgaaggt agcttcttat acgcttacgg cggaaaaggg gtagcgcgtc
     1681 cgcttctttc tctgccggtt tcccgcaggg ggcggtcccc ccgagtacat tcagagcgag
     1741 ctcttacagc gtgtgcactc ttttgccatg tcttgtgcgg agtacgatct tctgttcaaa
     1801 ctattacgag gtgagaaaaa tatgtcgaaa gtggtaggaa tcgatcttgg aacaaccaac
     1861 agctgcatcg ctgttcagga gggggacgtt acaacaataa tccccaatgc tgaaggaaac
     1921 cgcacaaccc cgtccgtcgt cgcgtttacg aaggaaggag agcgtcttgt cggccagctc
     1981 gccaagcgcc aggctatcgt gaacggcgac aggacgatca tgtccataaa gcgtgagatg
     2041 ggaactgacc acagagttac cgtcgacggg aaaaagtata ctccccagga gatctcttcg
     2101 atgatcctgc aaaagatgaa gcgcgacgcg gaggactatc tcggcgagcc ggtgacgaag
     2161 gctgtgatca cggttcccgc gtatttcacc gacgcgcagc gccaggcgac gaaggacgcg
     2221 ggggcgatcg ccgggcttga agtgctccgc attatcaacg agccgacggc agcatgcctc
     2281 gcctacggag agaacaagca gggcgagcat aagatccttg tattcgacct cggcggcgga
     2341 actttcgacg tgtcgatcct cgacg
//
LOCUS       DAOSWW010000391         2361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35718_length_2361_cov_3.810928, whole genome shotgun sequence.
ACCESSION   DAOSWW010000391 DAOSWW010000000
VERSION     DAOSWW010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35718_length_2361_cov_3.810928"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="PK442_08245"
     CDS             complement(<1..1569)
                     /locus_tag="PK442_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOO87679.1"
                     /translation="MGTRQPENTRSFAIAAHGGAGKTSLVEAMLFDSGSTSRLGKVED
                     GNTVSDFSQEEQKRQISINTSLITMERNGKTLFVLDTPGFADFVGEMRSAVRVADTLV
                     IAVSGTSGIEVQTDKAWQTGEEFGLPVAFYISKLDRENADFGKVLSDIKAVYSEKAVP
                     VFLPIGSESSFKGVVDILRSKAYIYEINGSGKFSEGDVPADMAADVASARETLVETVV
                     EADDEMMMRYLDGESISDDELLPVLRKAIASRLLLPVFPGSSTQNVGVHQILDFIVDF
                     FPSPLEARTRPAKKGDKDIEVAPDPSATFSAICFKVMVDPYVGKLSFIRVNSGTLTSD
                     NNIFNVNKGEDERISSFKFMTGKEGKDVKEIVVGDILTLPKLQSVQVGDTLGMKGSTV
                     IYPPIDFPKPVYSVAITAKSRADEDKLGNAINKTLEEDPSLSFEKNAETNDSVLSGMG
                     DMHIDIVLSKMKERYGVDLETKTPQVPYRETIRKTAEAQGKHKKQTGGHGQYGDAHIR
                     YTPLERGAGFVFEDN"
     gene            complement(1723..>2361)
                     /locus_tag="PK442_08250"
     CDS             complement(1723..>2361)
                     /locus_tag="PK442_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOO87680.1"
                     /translation="YFPDPDLPPIEASSALLEQVSASLPEMPWTREARFRNELGLPEA
                     DAALLAETPQLADYFESCARTGASPSRISNWVRTEVLRVLNERNISIDSFSMQPKTLG
                     SLVLLVDEGKLSTTAARETFNAMIEKGLPLEKALEASGGAGGKVSGDALADTIRAVLA
                     SAPDVVEEILGGKDDKGKKKKFLTGLVMKETRGQADAAAVGELIEKLLNEKP"
BASE COUNT          481 a          665 c          639 g          576 t
ORIGIN      
        1 attgtcctcg aacacaaatc ctgcgcctct ttcgagcgga gtgtaccgga tatgagcgtc
       61 tccgtattga ccgtgaccgc cggtctgttt cttgtgtttc ccctgggcct cggcagtttt
      121 gcggatggtc tcgcggtagg gtacctgggg cgtcttggtt tcaagatcaa cgccgtagcg
      181 ctctttcatc ttcgagagga cgatatcgat atgcatatcc cccatgcctg agagtacgct
      241 gtcgtttgtc tccgcgttct tctcgaagct gaggctggga tcttcctcaa gagtcttatt
      301 gatcgcattg cccagcttgt cctcgtcggc gcggctcttt gccgtgatcg cgacgctata
      361 gacgggtttt ggaaagtcga tgggaggata tattaccgtc gagcctttca tgccaagcgt
      421 gtcgccaacc tggacgcttt gaagcttcgg cagcgtgaga atgtcgccga cgacaatctc
      481 cttgacatct ttgccttcct tgcccgtcat gaacttgaag gagctgatac gctcgtcttc
      541 gcccttgttg acgttgaaaa tgttgttatc ggaagtgagg gttccggagt tgacgcggat
      601 gaaagagagc ttgccgacgt atgggtccac catgactttg aagcagatgg cggagaaagt
      661 ggcggacggg tccggagcga cttctatgtc tttgtcgccc ttcttcgcgg gacgcgtacg
      721 ggcttcgagc ggagacggga aaaagtcgac gatgaagtcg agtatctgat gcacgcccac
      781 attctgcgtg gatgagccgg ggaaaacagg gagcagaaga cgtgaggcaa tcgctttccg
      841 aagaactgga agcagctcgt cgtcggagat cgactctccg tcaagatagc gcatcatcat
      901 ctcgtcgtcg gcttccacta ctgtttctac cagcgtttct ctggccgaag caacgtcggc
      961 cgccatgtcg gcgggcacat cgccctcgga aaactttccg cttccgttga tttcatatat
     1021 atacgccttg ctgcggagaa tatcgacgac gcctttgaag gacgactcgc ttcctatcgg
     1081 aaggaagacg ggaacggctt tctcgctgta caccgccttg atatcggaga gcaccttgcc
     1141 gaaatccgca ttctcacggt ccagcttgga gatgtagaaa gccacgggaa gcccgaactc
     1201 ctctcccgtc tgccaggcct tgtcggtctg cacttcgatg ccgctcgttc cgctcacggc
     1261 gatgacaagg gtatcggcga cgcgtacggc cgagcgcatc tctcctacga aatcagcaaa
     1321 tcccggagta tccaggacaa agagggtctt cccattcctc tccatagtga taagagatgt
     1381 gttgatcgag atctgccgct tttgttcctc ctgactgaaa tcgctgacgg tgttgccgtc
     1441 ttcgaccttt cccagtctgg acgtactgcc gctatcgaaa agcatggcct caacgagact
     1501 ggtctttcct gcgcctccat gggccgcgat tgcgaaactg cgagtgttct caggctgacg
     1561 ggttcccatt gccaactacc tcctccggcg tttcgttcct gcctctgctc tactgcaggg
     1621 caacttgaat atcttacgca aaatgcctcc attagtcaac gaatcaaaaa aatctctttt
     1681 caaaagctgg taaaatacac cccttgagag aagacgaatg tttcagggtt tttcattcaa
     1741 caatttctcg atcaactccc cgacggcggc tgcatccgcc tgcccgcgag tctccttcat
     1801 aacgagtccg gttaaaaatt tcttcttctt gcccttatcg tctttgcctc cgaggatttc
     1861 ctccacgacg tcgggagccg atgcaagcac agcccggatg gtatcggcaa gcgcgtctcc
     1921 ggagaccttc cctcccgcgc ctccgctcgc ctccagcgcc ttttcgagag ggagcccttt
     1981 ttctatcatt gcgttgaaag tctcccttgc agcagtagtc gacagtttcc cctcgtccac
     2041 cagaagcacc agggaaccga gagttttcgg ctgcatagag aaggagtcga tggatatgtt
     2101 tcgttcgttc agaacacgga gcacttcagt acgaacccag ttcgaaatcc tggagggaga
     2161 ggcgccggtc cgggcgcagc tctcgaagta gtccgcaagc tgcggcgtct ccgcaaggag
     2221 agcggcatcc gcttcgggaa ggcccaactc gttgcggaag cgggcctctc tggtccacgg
     2281 catttcggga agggatgcac ttacctgttc cagtagtgcg gaagacgctt cgatcggagg
     2341 cagatcggga tcagggaaat a
//
LOCUS       DAOSWW010000392         2360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35738_length_2360_cov_4.730586, whole genome shotgun sequence.
ACCESSION   DAOSWW010000392 DAOSWW010000000
VERSION     DAOSWW010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35738_length_2360_cov_4.730586"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..475
                     /locus_tag="PK442_08255"
     CDS             176..475
                     /locus_tag="PK442_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87681.1"
                     /translation="MGLLIFALGIPSAMSLAGAPKVAGKDFLDAMDFISSNVLLPLGG
                     VFISLFVGWFWTSDAEKEVTNEGTLTFGLMSMWIWVCRVIAPAAILYIFYTGLKW"
     regulatory      640..742
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            862..1260
                     /locus_tag="PK442_08260"
     CDS             862..1260
                     /locus_tag="PK442_08260"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HOO87682.1"
                     /translation="MLVTNNPLLNEQISSRDFVEGNSLDVLIRTRNLIHKGWVLLTHP
                     LYGNLRPHQHPYRSILMERASDSSSSVDPLSVEYIENAIAIYSAQSSRILSVEGMPED
                     VRQDFAFIDADLMKESLSRYRMFIREVSVN"
     gene            1285..1605
                     /locus_tag="PK442_08265"
     CDS             1285..1605
                     /locus_tag="PK442_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOO87683.1"
                     /translation="MVELTKENFDVEVKESALPVLVDFWGPKCGPCMALLPNVHTMAD
                     EYEGKVKFCSVDVSENRRVAIANKVMGLPTFLFWKDGAEVTRISGGDVTLEKIKENVE
                     ALLK"
     gene            1642..>2360
                     /locus_tag="PK442_08270"
     CDS             1642..>2360
                     /locus_tag="PK442_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOO87684.1"
                     /translation="MKLELHKVKVNKLAWGDKTFAKGGVLTVNKEELLSVLSGDERLA
                     KVDVDFALPGESVRILPVKDVVEPRFKIEGPGGIFPGMVSDVDSVGEGKTLVLSGAAV
                     VTTGKIVRFQEGIIDMKGPGAEFTPYSQTCNVVVILENVEGIEKHDYETACRMAGLKT
                     AAYLAEKCLGASVDKVETYETPSLKDAMHAHPGLPKVAYLYMLQSQGLLHDTYLYGVD
                     VKKILPTFLNPTEVMDGAIVSG"
BASE COUNT          506 a          586 c          667 g          601 t
ORIGIN      
        1 gacgtttgtt acgctgccct ccgtttttgc gaagatgccc ggggcaatga tctagtccgg
       61 actcttcttc ctgcttctct tcatagcggc gctgacttct gcgatttctc tgctggaagt
      121 tgtttccgca tactttatcg acaaggggtg gagcagaccc caggctgcga tcatcatggg
      181 cctgctgatc ttcgcccttg ggattccttc cgccatgtct ctggcagggg ccccgaaggt
      241 tgccggaaag gacttcctgg acgcgatgga ctttatctcc tcgaacgtcc tgcttccgtt
      301 gggaggagtt ttcatctcgc tcttcgtagg ctggttctgg acctccgatg cggagaaaga
      361 ggtaaccaac gaaggcacgc tcacctttgg tctgatgagc atgtggatct gggtttgccg
      421 tgttattgct cccgcagcga ttctgtacat cttctacacc ggtttgaagt ggtaacggtc
      481 ctctgaactg cagtacgaag tgtatttcgc ttcgtacttt gtcgaggtga gaatgccgga
      541 atccgaagag attccggcat tttcgttttt tttgctccgt ctttcacaaa aagggcttga
      601 cgcctttttt tcattgtgta aaatgaagag tgtttccgga tgaacaccgc aagagagact
      661 ttcctgcgtt tgcggagagc gccgaagggg caataccctc tatagggtgg aaactctcag
      721 gcgaaaggat tgcggtcgga cgggtctctg aatagagtag ctcccctgtt tgtttttttc
      781 tctttccttt ggggctatgc gacagagaat gtcttatctg tgctctactt ttgaggaggc
      841 agccggatgt tctgtcgcag aatgcttgtc acaaataacc cgcttctgaa tgaacaaatt
      901 tcttcccgcg actttgtcga agggaattcc cttgatgtcc ttattcgaac aagaaatctc
      961 atccacaaag gatgggttct tcttacgcat cctctttacg gaaatcttcg tccgcatcaa
     1021 catccatacc gttctatcct gatggaacgg gcatcggatt catcgtcttc cgtcgatcct
     1081 ctctccgttg agtatatcga aaacgccatt gcaatttatt ccgcacaatc gtcacgaatc
     1141 ctctcggtcg agggaatgcc tgaggatgtg cgtcaggatt ttgcattcat cgatgctgat
     1201 ttgatgaagg aaagtctttc ccggtatcgt atgttcattc gggaagtttc ggtgaattga
     1261 gtctctaaaa ggaggtgaag agaaatggtt gaattgacga aggagaattt cgacgtcgaa
     1321 gtcaaggaga gcgcgcttcc tgtgcttgtc gacttttggg ggccgaaatg cggaccctgc
     1381 atggcgctgc ttcctaacgt ccataccatg gcggacgagt atgagggcaa ggtgaaattt
     1441 tgctcggtgg acgtatcgga aaatcgccgg gtggcgatcg cgaacaaggt tatggggctt
     1501 cctaccttcc tgttctggaa ggatggagcg gaggtgaccc gtatcagcgg cggagatgtc
     1561 acgttggaga agatcaagga gaacgtcgag gctctcttga agtagccttc gacgcctgta
     1621 aaagggggga gacagacagc catgaaactt gagctgcaca aggtaaaagt aaacaaactt
     1681 gcttggggcg acaagacctt cgccaagggc ggggtgttga cggtgaacaa ggaggagctt
     1741 ctttccgttc tttccggaga cgagcgtctt gcgaaagtgg acgtcgattt tgcgcttccc
     1801 ggcgagagcg tccgcatcct tcccgtgaag gacgtcgtgg agcctcgatt caagatcgaa
     1861 ggcccgggcg gaatattccc gggcatggtc tccgatgtcg attccgtggg cgagggcaag
     1921 acgctcgtcc tctccggagc ggccgtagtg accacgggga agatcgtccg tttccaggaa
     1981 ggcatcatcg acatgaaggg acccggagcg gagtttactc cgtattctca gacctgcaac
     2041 gtcgtcgtga tcctggagaa cgtcgaggga atcgaaaagc acgactacga gacggcatgc
     2101 aggatggccg gactgaagac cgccgcgtat cttgccgaga agtgccttgg cgcttcggtc
     2161 gacaaagttg aaacctacga aacgccctcg ctgaaagacg ccatgcacgc gcatcccggg
     2221 cttccgaagg tcgcctatct gtatatgctc cagtcgcagg ggctgctcca cgacacgtat
     2281 ctctacggtg tggatgtgaa gaagattctt cccacgttcc tcaacccgac cgaggtcatg
     2341 gacggcgcca tcgtctccgg
//
LOCUS       DAOSWW010000393         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35789_length_2358_cov_3.393400, whole genome shotgun sequence.
ACCESSION   DAOSWW010000393 DAOSWW010000000
VERSION     DAOSWW010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35789_length_2358_cov_3.393400"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..345
                     /locus_tag="PK442_08275"
     CDS             37..345
                     /locus_tag="PK442_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87685.1"
                     /translation="MNRQVFWEKLKSTLHNTWEFIVLFHHGTFVDKRMAVVRKEAFDI
                     NDNLMLLLFGDYLGVPNPMSYYMLELLPYVANDLEPWERRIQNRKFLIAEKAAQYDFD
                     "
     gene            359..1306
                     /locus_tag="PK442_08280"
     CDS             359..1306
                     /locus_tag="PK442_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOO87686.1"
                     /translation="MRRFTFFGGKGGTGKTSCAAAYALSLASKGIRTLVVSTDPAHSL
                     ADAFGKPIGFETALLRENLWGLEIDAEVEAKKYMKVIQEKVLDLVSAAIVEEIKRQIE
                     IAYMSPGAEEAAIFDKFVELMESAGTTYDAIVFDTAPTGHTLRLLTLPQILETWIEHL
                     IEKRRKAMHLMKMAARYDSVLADKIKEDPIIETLSARKEKFALARRYLTDSSLSGFFF
                     VLNAEKLPILETARAVELLGKHGVHVGGMVINKVIPPEAGAFFEKRRSAQEGYLADIN
                     SRFSSFVRVELPLLDSDIQGVEQLEMMVPLLGALDASGE"
     gene            1314..>2358
                     /locus_tag="PK442_08285"
     CDS             1314..>2358
                     /locus_tag="PK442_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOO87687.1"
                     /translation="MKLKIACVQMRARSAEHRGEALQSALRMIDSAADGGAKMALLPE
                     TFYPSYYLGGIDPAWSWREAFSALSEKAAERRIFLAAGIVLKDGGSLRNAAVLWGPDG
                     KERLRTYKSNLWHFDERYVEPGLSFDVAETPWGPVGMMICADGRIPEIARILALKGAR
                     LILDPTNLVASGRDPSRLSSPQLEYMLCARAAENGLWIAVANKVGLESESVLNCGGSC
                     VVDPYGEKVASLGSAEEGILFVDVDLDAAPYALPARAPQKYGAIAQKKENTRAFQSLS
                     EPLPPLAEDELFLGVAQFSYEGTDDYMNRAARMLTRSADQGASLVCLPGTPSCSGDEI
                     AAALSSSLAGEKCM"
BASE COUNT          477 a          662 c          751 g          468 t
ORIGIN      
        1 ggagtgctca acatagacaa agccggcaag tagccgatga acagacaggt tttttgggaa
       61 aagctgaagt caacgcttca caatacgtgg gagttcatcg tcctcttcca ccacgggacg
      121 tttgtggaca aacgcatggc ggttgtccgc aaggaggcgt tcgatatcaa cgacaatctg
      181 atgcttttgc tcttcggcga ctatctcgga gtgcccaatc cgatgtcgta ctacatgctc
      241 gaactccttc cgtacgtggc gaacgacctg gaaccgtggg aacggcgcat ccagaaccgc
      301 aagttcctca tcgccgagaa agcggcgcaa tacgatttcg actgagggga gaaccctgat
      361 gagacgattt acatttttcg gcggcaaggg aggaacggga aagacctcct gcgctgccgc
      421 ctatgctctt tcactcgcaa gcaaaggaat ccggacgctt gtggtttcca ccgacccggc
      481 gcactccctt gcggatgcgt tcggcaaacc gattggcttc gagacggcgc ttctgcgcga
      541 aaacctctgg gggcttgaga tagacgccga ggtcgaggcg aagaagtaca tgaaggtcat
      601 tcaggagaaa gtccttgatc tggtgagcgc ggcgatcgtc gaggagatca agcgtcagat
      661 cgagatagcc tatatgtcgc ccggagcgga ggaggcggcg attttcgaca agttcgtcga
      721 gcttatggag tctgcgggaa cgacctacga cgcgatcgtc ttcgataccg cgccgacagg
      781 ccatacgctc cggcttctga cgcttcccca gatcctggag acgtggatcg agcacttgat
      841 cgagaagcga aggaaagcga tgcacctcat gaagatggcc gctcggtacg acagcgtcct
      901 cgccgacaag atcaaggaag acccgatcat agaaacactt tccgcgcgga aggagaagtt
      961 cgcccttgcg cgccgctacc tgaccgactc gtctctttcc ggctttttct tcgtcctcaa
     1021 cgcggaaaag cttccgattc tggagaccgc gagggcagtc gagcttcttg gaaaacacgg
     1081 tgtccacgtt ggcggtatgg tgatcaacaa ggttatccct ccggaggcgg gcgcattttt
     1141 cgagaagaga cgaagcgccc aggaaggcta tctggcggat atcaacagca ggttctcctc
     1201 gttcgtccgc gtggaactgc cgctgctcga ctcggatatt cagggcgtcg agcagctgga
     1261 gatgatggtt cctcttctcg gcgcgcttga tgcgtccgga gagtagcggc gccgtgaaac
     1321 tcaagattgc ctgcgtacag atgcgcgcgc gttccgcgga acaccggggc gaggcgcttc
     1381 agagcgcgct ccggatgatc gactctgcgg cggacggcgg ggcgaagatg gcgctgctgc
     1441 cggagacgtt ctacccttcg tactacctcg gggggattga ccctgcctgg tcgtggaggg
     1501 aggcgttctc agccctctcg gagaaagccg ccgagcggag gattttcctc gccgccggaa
     1561 tcgtcctcaa ggacggggga agtctcagga acgccgcggt gctctggggg ccggacggga
     1621 aggagcgtct gcggacatac aaatcgaatc tctggcactt cgacgagcgg tacgtcgagc
     1681 ccgggctctc tttcgacgtt gccgaaacgc cctggggtcc ggtcggaatg atgatatgtg
     1741 ccgacggccg gattccggag atcgcgcgga tacttgcgct gaagggagcg cgcctgatac
     1801 tggacccgac gaacctcgtc gcttcgggca gagacccgtc gcgcctgtcg agtccgcagc
     1861 tggagtatat gctctgcgcc cgggctgcgg aaaacggtct gtggatcgcg gtcgcgaaca
     1921 aggtcggact ggagtctgaa tctgtcctga actgcggcgg cagctgcgtt gtggacccct
     1981 acggcgagaa ggtcgcttcg ctggggtctg ccgaagaggg gatcctgttc gtcgacgtcg
     2041 atctggacgc cgcgccgtat gccctgcctg cacgcgcccc tcaaaagtac ggcgcgattg
     2101 cgcaaaagaa ggagaatacg agggcgtttc agagcctttc cgagcctctt ccgccgcttg
     2161 cggaggacga gctctttctc ggggttgcgc agttctcgta tgagggaacg gacgactaca
     2221 tgaatcgcgc tgcgcgtatg ctgacgagaa gcgccgacca gggagcgtcg ctggtctgtc
     2281 ttcccggaac gccttcctgt tcgggtgatg agatcgccgc cgcgctttcc tcctcgcttg
     2341 ccggagaaaa atgcatgg
//
LOCUS       DAOSWW010000394         2355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_35885_length_2355_cov_3.092609, whole genome shotgun sequence.
ACCESSION   DAOSWW010000394 DAOSWW010000000
VERSION     DAOSWW010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35885_length_2355_cov_3.092609"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK442_08290"
     CDS             <1..462
                     /locus_tag="PK442_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87688.1"
                     /translation="VGTIMGIKTSLATLDGWQHIILPSVTLALGTLATIIRLTRSGMQ
                     ENMKQDYVRFAKSKGVPRRTVLFGHALKNTLIPVVTIFGMQMGTLIAFTTITETIFAW
                     PGMGKLLIDSINSLDRPIIAAYILFVAVMFVFINFIVDILYVFIDPRIDLQ"
     gene            479..1378
                     /locus_tag="PK442_08295"
     CDS             479..1378
                     /locus_tag="PK442_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87689.1"
                     /translation="MTNTARSGRWRAFLDSEFFHNYRRSPSAVIGSILVLFVLLVAIF
                     GPLWTVQNPYNIASLELRDAYKPPAWIEDGEARFLLGTDQQGRDMLSAIVYGSRVSLI
                     IGVAGTLLASAIGIVLGLVAGFFGGRTDAIIMRIADIQLSFPTMLIALFLMSVLGRGV
                     TNILISLTLVGWVRFARTVRGETLSVKKREYVEAARVIGLPNIRILFKHVLPNVFASI
                     IVLATIQVGNFILTEATLSFLGLGVPITRPSLGMLCNNGFTVLYSGLWWVSILPGLYI
                     MIIVFGINLLGDFLRDELNPKLK"
     gene            1394..>1967
                     /locus_tag="PK442_08300"
     CDS             1394..>1967
                     /locus_tag="PK442_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87690.1"
                     /translation="MNDTLLEVKNLRTYFHTFKGTVKAVDDVSFSVGRGEILAIVGES
                     GGGKSVTGFSVIRLIEEPGVIESGEILFEGRDVMKLSEREVDRLRGKEISMIFQDPMT
                     SLNPVYTIGRQIEESLLLHTKLNREERRAACIDLLRSVGISNPESRLKAYPHQFSGGM
                     RQRVVIAIALAADPKLIIADEPTTALDVTIQ"
     assembly_gap    1968..1977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1978..>2355
                     /locus_tag="PK442_08305"
     CDS             <1978..>2355
                     /locus_tag="PK442_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87691.1"
                     /translation="ILRLMADLVRAKNRSMILVTHDLAVVSEMADRINVMYCGKIVET
                     GSARKVIDESAHPYTKGLIDSIPDLERDRKRLNTIKGIVPNMFELPEGCNFAPRCARA
                     TEFCFRNQPEQREIGAGHFVACHF"
BASE COUNT          442 a          768 c          689 g          446 t
ORIGIN      
        1 gtaggaacga tcatggggat caagaccagt ctcgccacgc tcgacggctg gcagcatatc
       61 atcctcccgt cggtcacgct ggcgctgggg acgctcgcca ccatcatccg cctgacgcgg
      121 tccgggatgc aggagaacat gaagcaggac tacgtgcgct tcgccaagtc caagggcgta
      181 ccgcgccgca ccgtgctttt cggtcacgcg ctgaagaaca cgctcatccc cgtggtcacc
      241 atcttcggca tgcagatggg aaccctcatc gccttcacca ccatcacgga gaccatcttc
      301 gcctggcccg gcatggggaa gctgctgatc gactcgatca acagtctcga ccggccgatc
      361 atcgcggcgt acatcctctt cgtggcggtg atgttcgtct tcatcaactt catcgtggat
      421 attctctacg tcttcatcga tcccaggatc gatcttcagt gaaagggggc ggcgtataat
      481 gacgaatacc gcgcgctccg gccgctggag agcctttctg gattcggagt tcttccataa
      541 ctacaggcgc tctccctccg ccgtcatcgg ctccattctt gtcctcttcg tccttctggt
      601 cgccatattc ggccctctgt ggacggtgca gaatccgtac aacatcgcgt ccctcgaact
      661 tcgggacgcc tacaagcccc ccgcgtggat cgaggacggg gaggcgcggt tcctcctggg
      721 aaccgaccag caggggcgcg acatgctcag cgccatcgtc tacggcagca gagtctccct
      781 catcatcggc gtggccggaa cgctgctggc aagcgcgatc ggcatcgtgc tcgggcttgt
      841 ggccggtttc ttcggcggcc gtaccgacgc gatcatcatg cggatcgcgg acattcagct
      901 ctccttcccg accatgctca tcgcgctctt cctgatgtcc gtcctcggaa ggggcgtgac
      961 caacatcctc atctcgctga cgctggtggg gtgggtccgc ttcgcaagga cggtgcgcgg
     1021 cgagacgctc tcggtcaaga agcgcgagta cgtcgaggcc gcgcgtgtca tcggtcttcc
     1081 gaacatcagg atactcttca agcacgttct gcccaacgtc ttcgcctcga tcatcgtcct
     1141 cgccacgatc caggtgggca acttcatcct cacggaggcc accctgagct tcctcggact
     1201 gggcgtcccc atcacccgcc cctctctggg aatgctctgc aacaacggct tcacggtgct
     1261 ctacagcgga ctctggtggg tctccatcct gccggggctc tacatcatga tcatcgtctt
     1321 cggcatcaac cttctgggcg acttcctgag ggacgagctg aacccgaagc tcaagtgagg
     1381 agggacggac cgaatgaacg acacgctgct tgaagtaaag aatctgcgca cgtacttcca
     1441 cacgttcaaa gggaccgtca aggcggtcga cgacgtctcc ttctccgtcg ggcggggcga
     1501 aatcctcgcc atcgtcggcg aatcgggagg cggaaagtcc gtcaccggct tttccgtcat
     1561 ccgcctgatc gaagagcccg gcgtcatcga gtcgggggag atcctcttcg agggccgcga
     1621 cgtgatgaag ctctccgagc gcgaggtgga ccgtctccgg gggaaggaga tctccatgat
     1681 cttccaggac ccgatgacgt cgctgaaccc cgtgtacacc ataggacggc agatagagga
     1741 aagtctgctg ctccacacga agctgaacag ggaggagcgg cgcgcggcct gcatcgacct
     1801 gctgcggtcg gtcggcatct ccaaccccga gagcaggctc aaggcctacc cgcaccagtt
     1861 cagcggcggc atgcggcagc gggtggtcat cgccatcgcg ctggccgcgg accccaagct
     1921 gatcatcgcc gacgaaccca ccacggcgct cgacgtgacg atacaggnnn nnnnnnnatc
     1981 cttcgcctga tggccgacct tgtcagggcg aagaaccgct cgatgatcct ggtcacccac
     2041 gacctcgccg tcgtctcgga gatggccgac cggatcaacg tgatgtactg cggcaagatc
     2101 gtggagaccg gttccgcgcg gaaggtcatc gacgagagcg cgcacccgta cacgaagggg
     2161 ctgatcgact ccatccccga cctggagcgc gacaggaaga ggctgaacac catcaagggc
     2221 atcgttccca acatgttcga gcttcccgaa gggtgcaact tcgccccacg ctgcgcgcgc
     2281 gccacggagt tctgtttcag gaaccagccg gaacagagag aaatcggggc ggggcatttc
     2341 gtcgcctgcc acttc
//
LOCUS       DAOSWW010000395         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36005_length_2350_cov_3.593028, whole genome shotgun sequence.
ACCESSION   DAOSWW010000395 DAOSWW010000000
VERSION     DAOSWW010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36005_length_2350_cov_3.593028"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PK442_08310"
     CDS             <1..606
                     /locus_tag="PK442_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HOO87692.1"
                     /translation="PPRFTLVGATTRLGLLTSPLRARFGIVEQLSLYSEDELRSIVAR
                     GARVLGVAIHDDAALEIARRSRGTPRIALRLLRRVRDVAEVRALTSVDSATAFSALNM
                     LGLDDLGLDEGDRRILRVIVELFDGGPVGLSTLGAALNEETQTIEDIYEPFLIQQGLL
                     ERTPRGRKATKNCWIYLGKTPPPEGNEQWQLHLPVEEGAEG"
     gene            666..2081
                     /locus_tag="PK442_08315"
     CDS             666..2081
                     /locus_tag="PK442_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOO87693.1"
                     /translation="MFLLRKKCFFRRALLLVSILSIVMLPQTAAARTVSVGLAIGQSS
                     VSVSSSSALSATDGSGKKHALGTTAVFRSAGTNSVVAGNRTLRLPVSISSRHPLSFNK
                     RRYRGGFRLVSSGSGITLLNVLDLEEYLRGVLKMEVNPAWSMEALKAQAVISRTYALR
                     SVLQNRGKGGYDLRDTVMSQVYRGMNAEDPKTDQAIRATKGMVLKYGGEIAFTPFHSH
                     SGGATANVTTVWGGNMPYLKGVNEDFQTESPHSTWEASLSAAQVEDALRSAGVNVGRL
                     QELRVEQSDPFGRPTTLRAIGTSGSKTLKSHSFRMAAGSNVIRSTLFSISSAASSSSA
                     PIPLASLSQPKPVPPSPPAGTGVPTSLTPMSAAEERQLTDLTEQGVFNAEELMDMLLN
                     PEKRKGYLIRALRTVRPGIPVQPQPQPVGTASGRFLFRGKGWGHGVGLSQWGAKALAD
                     QGWDFKKILSFYFPGTKLEGM"
     gene            2091..>2350
                     /locus_tag="PK442_08320"
     CDS             2091..>2350
                     /locus_tag="PK442_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOO87694.1"
                     /translation="MKTVDLFDIESYNYMLPEELIAQNPATPRDSSRMLVLDRFSGKM
                     MHRRFSDLRELLKPSDLLVLNDTRVIPARLRGSKLPGGGKAEV"
BASE COUNT          493 a          616 c          694 g          547 t
ORIGIN      
        1 cccccgaggt ttacccttgt cggcgcgacg actcggctcg ggttgctcac ctcgcctctt
       61 cgtgcgcgtt tcggaatagt tgagcagctc tctctctatt cggaagatga actcaggagt
      121 attgtcgcgc gtggcgccag ggtgctcggt gtagcgatac atgacgacgc ggcgcttgag
      181 atcgccaggc gttcgcgcgg cactccgcgt atcgcgcttc gtcttcttcg cagggtccgc
      241 gacgtcgcgg aggtccgcgc ccttacatct gttgacagcg caacggcttt ttctgctctt
      301 aatatgctcg ggttggatga tctggggctt gatgagggtg atagaagaat tctcagagtt
      361 atcgtcgaac tgttcgacgg cggacctgtc ggcctttcga cgcttggcgc cgcgctcaac
      421 gaggagaccc agacgatcga agatatctac gaaccttttt tgatccagca ggggcttctt
      481 gagcgaacgc cccggggacg gaaagcgacg aaaaattgtt ggatatatct cgggaaaaca
      541 cctccgcccg aggggaacga gcagtggcag ttgcatcttc ctgttgagga gggggctgaa
      601 ggatgaattc tccgtgaaaa atagtagtcc tggattgatg aaataagaaa aatgggggta
      661 agccaatgtt cttactgaga aaaaagtgtt tcttccgccg agctcttctt ctagtttcaa
      721 ttctgagtat tgttatgctg ccgcagacgg cggcagccag aactgtttct gtcggtctcg
      781 ctatcggcca gtcatctgtt tcggtttcct cctcttctgc tctttccgcg acggatggga
      841 gcgggaaaaa acatgcgctg ggtacaacag cagtattccg tagtgcggga acgaacagtg
      901 ttgtcgcggg aaaccgtacg ttacgtctgc ctgtgagtat ctcctcgcgc cacccgcttt
      961 ctttcaataa aagacggtat cgaggcggat tccgcctggt atcttcaggc tcggggatta
     1021 cgcttctgaa cgtcctcgat cttgaggagt accttcgggg agtgttgaag atggaggtga
     1081 atcctgcctg gtccatggaa gcgctcaagg cgcaggcagt catctcaaga acgtatgctc
     1141 ttcgaagcgt ccttcagaac aggggcaagg gggggtacga tcttcgcgat accgttatgt
     1201 cgcaggtcta ccgcggcatg aacgctgaag accccaagac cgaccaggct atacgcgcga
     1261 caaagggaat ggtgttgaaa tacggcgggg agatcgcatt cacccctttc cattcgcata
     1321 gcgggggagc gactgccaat gtgacgacgg tgtggggcgg aaacatgccg tatctcaagg
     1381 gtgtcaatga agattttcag acggagtctc cgcattcaac atgggaagcc agtctttcag
     1441 cggcccaggt tgaggacgct cttcgttctg caggagtgaa cgtagggcgt cttcaggagc
     1501 ttcgggtgga gcagtcggat ccgttcggca ggccaacaac gctccgcgcc atcgggacga
     1561 gcgggagcaa gacgctcaag agtcacagct tccgtatggc cgcagggagc aatgtgatca
     1621 ggagtacgct cttttccatc tcgtccgcag catcctcctc ttcggctcct attccccttg
     1681 cttcgctttc tcagccgaag cctgttcccc cttcgcctcc cgcaggaacg ggcgtcccca
     1741 cgtcgctgac gccgatgagc gcggcggaag aaagacaact gaccgatctt accgagcaag
     1801 gggttttcaa cgctgaagag ctgatggata tgttgctcaa ccctgaaaag cgaaaggggt
     1861 accttatccg tgcactgcgt actgtacgtc ccggtatccc tgtacagccc caaccgcaac
     1921 ccgtcggtac ggcttccggc aggtttcttt tccggggcaa ggggtggggg catggcgtcg
     1981 gactctccca gtggggggcg aaggcgcttg cagaccaagg gtgggatttt aaaaagattc
     2041 tttcgttcta ctttcccggg acgaaacttg aagggatgta gaccgcgttt atgaagaccg
     2101 tcgatctttt cgacatagag tcgtacaatt atatgttacc ggaggaactc attgcacaga
     2161 atccagcaac acccagggat tcatcgcgaa tgctggtatt ggaccgtttt tccggaaaga
     2221 tgatgcaccg tcgtttttcc gatctacggg agctgctgaa gccgtccgat ctcttggtcc
     2281 tcaatgacac gcgcgttatt cctgcgcgtc tgcgaggaag caagcttccc ggaggaggga
     2341 aggcggaagt
//
LOCUS       DAOSWW010000396         2344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36178_length_2344_cov_2.470511, whole genome shotgun sequence.
ACCESSION   DAOSWW010000396 DAOSWW010000000
VERSION     DAOSWW010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36178_length_2344_cov_2.470511"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PK442_08325"
     CDS             complement(<1..488)
                     /locus_tag="PK442_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOO87695.1"
                     /translation="MRLITRSDFDGLICGMLLKEAGIIDSWQFVHPKDLQDGLFQPTE
                     NDVLANVPYVPGCGMSFDHHTSENDRVGWSKDVQGESRLAPSAARIIYEYYGGAEKFP
                     AYGDMIEAVDKVDSGQLTREEILNPKGWILLGFLTDPRTGLGRFREFRISNYQLMEEL
                     IN"
     gene            655..>2344
                     /locus_tag="PK442_08330"
     CDS             655..>2344
                     /locus_tag="PK442_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO87696.1"
                     /translation="MVLFYNNDEEDASMTDMRRKTTACPYDCPDACAMKGMFDGASVT
                     LEPNRDLPYTTFLCGKGLRWASRATSSERLTTPMLRREGALVPVSWDEAVDVWAEKVR
                     SAVSRHGPLSLMHLSSAGSLYFSKLLLPHLFAALGGYTTKKGNLCSSAGSFGLAESFG
                     EVPVTRPESLSDHARGVLFWGRNALETHAHVVPLLQRVRGRGGEIASLEIRETPTTRF
                     ADRWWRVNPGGDWALAAWLCARLLEEGASDGWRERVENPEEFERTARSLDGAFLLRTA
                     GVSAECASELLEWLMRFAPVTHYAAFGAQRYLHGDAQFRWIGALAVLLGAFSGPGAGL
                     AFSKDEMALFPSALLPKVAYVRRLPVATWPVELESLDPPVEALSISGANPVRQSPGSD
                     FVREALRKIPFTVCIDFVLSDTAKECDLVLPTTTFLEEEGDWKGSYWHNYVVRSERIL
                     PPRGESLEETEIFTRLARRLGLPLDLTERKREVDRAMLASPLLERVGEGVFRWDEPDY
                     WRASQRKAALPVSVPEAAEGREGALRLVTVHRKEYINGQSWDAPSVEALPPLSVH"
BASE COUNT          353 a          753 c          812 g          426 t
ORIGIN      
        1 tggttgatca gctcctccat gagctggtag ttgctgatgc ggaactcgcg gaatctgccg
       61 agccccgtcc tgggatcggt gagaaagccg aggaggatcc accccttcgg attcagtatc
      121 tcctcccggg taagctgccc cgaatccacc ttgtccacgg cctcgatcat gtcgccgtag
      181 gcgggaaact tctccgcgcc gccgtagtat tcgtagatga tccgcgccgc ggaaggcgcg
      241 agtcggctct cgccctgcac gtccttcgac cagccgacgc ggtcgttctc cgacgtatgg
      301 tggtcgaacg acatcccgca gccggggacg tacggaacgt tcgccagcac gtcgttctcc
      361 gtcggctgaa agagtccgtc ctgaagatcc ttcgggtgga cgaactgcca cgagtcgatg
      421 atccccgcct ccttgagcag cataccgcag atcaggccgt cgaaatcaga gcgtgtgatc
      481 aatcgcatgt cctaccaact ccccgtctat ttttcgcttg cagagcgttc cccatctctt
      541 gggcggatcc cgggtgggac cgccgtcctt ttcgcgcagg gcttccgggc gtaggggccc
      601 cgggcgctct ttccgttcct gccgcaaaga aagcctgtcg gaagcgcgcc gtcaatggta
      661 ttattctaca acaacgacga ggaggatgca tcaatgaccg atatgcgccg aaaaacgacc
      721 gcgtgtccct acgactgccc ggatgcctgc gcgatgaagg gcatgttcga cggggcgtcc
      781 gtgaccctgg agccgaatag ggatctgccg tacaccacct tcctgtgcgg gaaggggctg
      841 cgctgggctt cgagggcgac gagttccgaa cggctgacga cgccgatgct ccgcagggag
      901 ggcgcgctcg tccccgtctc ctgggacgaa gcggtggacg tctgggcgga gaaggtccgt
      961 tcggcggtct cccggcacgg gccgctgtcg ttgatgcacc tttcgagcgc aggctcgctc
     1021 tacttctcga agctcctgct gccgcacctc ttcgccgcgc tcggcgggta cacgacgaag
     1081 aagggaaacc tctgctcttc ggcggggagc ttcggccttg cggagagctt cggcgaagtg
     1141 cccgtcacgc gtccggagtc gctttcagac catgcgcgcg gcgtcctctt ctgggggcgg
     1201 aacgcgctgg agacgcacgc gcacgtcgtc ccgctgctgc agcgggtgcg cggccggggg
     1261 ggcgagatcg cctcgttaga gattcgcgag acgccgacaa cgcgcttcgc cgaccggtgg
     1321 tggcgggtga acccgggcgg cgactgggcg ctggcggcgt ggctctgcgc acggctcctc
     1381 gaggaagggg cgagcgacgg gtggcgggag cgcgtcgaga atccggagga gttcgagcgc
     1441 acggcgcggt cgctcgacgg ggctttcctg cttcgtacgg cgggcgtctc cgcggagtgc
     1501 gcgtcggagc ttctggagtg gctgatgcgc ttcgcccccg tgacgcacta cgcagccttc
     1561 ggcgcgcaac ggtatctgca cggcgatgcg cagttccgct ggatcggggc gctcgccgtc
     1621 ctcctggggg ccttctcggg gccgggcgcg gggcttgcct tcagcaagga cgagatggcg
     1681 ctctttcctt ccgcccttct gccgaaggtt gcgtatgtgc gcaggcttcc cgtcgccacg
     1741 tggccggtgg agctggagtc gctcgatccc cctgtggagg cgctctccat ctccggtgcg
     1801 aatcctgtgc gccagagtcc ggggagcgac ttcgtgcgcg aggcgctccg gaagatcccc
     1861 ttcacggtct gcatcgactt cgtcctctcc gacacggcga aggagtgcga tctcgtgctc
     1921 ccgaccacga ccttcctcga agaggagggg gactggaagg gttcctactg gcacaactac
     1981 gtcgtcagga gcgagcgcat cctcccgccg cggggagagt cgctggagga gacggagatc
     2041 ttcacccgcc tcgcgcgccg gctcgggttg ccgctcgacc tgacggaaag gaagcgggag
     2101 gtggaccggg ccatgctcgc ctcgccgctg ctggagcgtg tgggggaggg ggtcttccgg
     2161 tgggacgagc cggactactg gcgcgcctcg cagcggaagg cagcgcttcc cgtgtcggtt
     2221 cccgaggcgg cggagggacg ggagggcgcg ctccggctgg tcacggtgca tcgcaaggag
     2281 tatatcaacg gacagagctg ggacgccccg tccgtcgaag cgcttccccc gctctcggtg
     2341 cacc
//
LOCUS       DAOSWW010000397         2341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36250_length_2341_cov_4.136483, whole genome shotgun sequence.
ACCESSION   DAOSWW010000397 DAOSWW010000000
VERSION     DAOSWW010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36250_length_2341_cov_4.136483"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PK442_08335"
     CDS             complement(<1..659)
                     /locus_tag="PK442_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOO87697.1"
                     /translation="MTMHNENTFQIENPHALGAQEALEQLRVTPEGLKREDAAGRLRS
                     VGPNRLPEPPKEGVLKRFFKHFHDLLIYILIAAAFITAVLGHFVDTGVILAVVVINAI
                     IGFLQEGKAEEALEGIRKMLSLTAHVRRGGEWEETGAEELVPGDIVRLRSGDRVPADV
                     RLLETVNLRIEEAALTGESVPSGKDTEAVSSGAGVGDRSCMAYSGTMVTAGRGTGVVT
                     AT"
     gene            969..2009
                     /locus_tag="PK442_08340"
     CDS             969..2009
                     /locus_tag="PK442_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO87698.1"
                     /translation="MPVTLKQLAERTGKSITTVSRALAGYDDVSEETKKLVRRVADEL
                     HYTPNFLARSLQKRRSRTISLILPTDSPRFSDPFFSEFLTGVANMAAERRYDLLLSVQ
                     APGEGEMELYQRKVSSGQVDGFVVVRTRRRDPRIAWLYKSQVPFVAFGRTENGADFPF
                     VDVDGVKGMRLIGEHLIGLGHRRIVFLSSPSELLFVEHRLDGLRQAMTTHGLDPDEMR
                     ILQGDLTQKSGYEATASLLDGGNPPSAIVACNDLMALGAMSALHERGISVGPDIAVTG
                     FDDIPMAEHAHPPLTTIHQPIYEIGRMVCEKLVRLIAGEELASRSEILEPRLIVRSSC
                     GSLRKQPDQTGR"
     gene            2143..>2341
                     /locus_tag="PK442_08345"
     CDS             2143..>2341
                     /locus_tag="PK442_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87699.1"
                     /translation="MRKHVKRTRAFAALLTLSAILWCGAAFAAGLSGLVFFSTQFAPV
                     EEQAKFREILKDGGFDYTADSN"
BASE COUNT          496 a          752 c          645 g          448 t
ORIGIN      
        1 gtcgccgtga ccacgccggt cccccgcccc gcagtcacca tcgtgccgga ataggccatg
       61 caggaccggt cgccgacccc ggcgccggac gaaacggcct cggtatcctt cccggaggga
      121 accgactctc cggtaagcgc ggcctcctcg atgcggagat tcactgtttc cagcagacgc
      181 acatcggcgg gaactctgtc ccccgagcgc agacggacga tatcgccggg gacaagctct
      241 tcagcgcccg tctcctccca ctctcccccc cgccgcacat gcgcggtaag ggagagcatc
      301 ttccggatac cctcaagggc ctcttccgcc tttccctcct gcagaaaacc tatgatagcg
      361 ttgatcacca ccacggccag aataactccg gtgtcgacga agtgccccaa aactgcggtg
      421 ataaaggccg cggcaatcaa aatatagatc agcaagtcgt gaaagtgctt gaagaagcgc
      481 ttcagcaccc cctccttcgg cggttccggc agcctgttgg gaccgaccga acgcagacgc
      541 ccggcggcgt cttcacgctt caacccctca ggtgtaactc gaagctgctc aagcgcttcc
      601 tgcgccccaa gcgcgtgcgg attctcaatt tgaaatgtat tttcgttgtg catcgtcata
      661 ctgcaccccc ctttgtgttt cacaatgcat tttggttatt tcttaatgag cagttacaat
      721 ctattgttct aggataactt taaatgccga atatttcaag gataatcgtt tctcgacgct
      781 ccgcactcgt ccgcacacac gctcccgttt caccggagga cacaaagaag cgtcacaaat
      841 gaagaggaaa catgaagagg aaacgcttct tgacatccaa aacgttttgg tttagagtac
      901 acggcacatt caaaacgttt tggttcatct atcggaacaa acgtcgactt ctcggggagg
      961 aaccgactat gccggtcaca ctcaagcagc tcgcagaacg caccggaaaa tccatcacta
     1021 ccgtctcgcg cgcactggcg gggtacgacg acgtcagcga ggagacgaaa aagctggtgc
     1081 gacgcgtggc ggacgagctg cactacacgc ccaacttcct tgcccgttcc cttcaaaagc
     1141 gccgctcccg gacgatcagc ctcattctcc cgactgacag cccgcgtttc tccgatcctt
     1201 tcttcagcga atttctgacc ggcgtcgcca atatggcagc ggaacgcagg tacgatctgc
     1261 tgctctccgt gcaggcgccg ggagaagggg agatggagct ctaccagcgc aaggtaagca
     1321 gcggccaggt ggacggcttc gtcgtcgtcc gcacccggcg ccgcgacccc cgtatcgcat
     1381 ggctctacaa atcccaagtc cccttcgtcg ccttcggacg aaccgaaaac ggcgcggatt
     1441 tcccctttgt agatgtggac ggcgtcaagg ggatgcgtct gatcggagaa catctcatcg
     1501 gcctcggcca ccggcgcatc gtctttctga gctccccctc cgaactgctc tttgtcgagc
     1561 accggctcga cggtctgcgc caggcgatga ccacgcacgg cctcgatccc gacgaaatgc
     1621 ggatactcca gggagatctc acccagaaga gcggatacga ggcgaccgcc tcgctgctcg
     1681 acggcgggaa tcctccctcc gcgatcgtag catgcaacga cctgatggct ctgggcgcga
     1741 tgagcgcgct ccacgagcgc gggatctccg tcggtccgga catcgccgtc acaggtttcg
     1801 acgacatccc gatggcggag cacgcgcacc ctccgctgac cacgattcat caacccatct
     1861 acgaaatagg acgaatggtc tgcgaaaagc tcgtccggct gatcgccggc gaggaattgg
     1921 cctcccgcag cgagatactg gagccgcggc tgatcgtacg ctcctcctgc ggctctctcc
     1981 ggaagcagcc ggaccaaacg gggaggtgat gcggcgcgga ccttcatcca cagaatgaca
     2041 cgctgcaata cggcggtaag acaacgggct cccgccgcct gcccgatcgt tccagggagt
     2101 gtttttcagg cgtgggagga tgtgtgtaca aggaggaatg aaatgagaaa gcacgtgaaa
     2161 agaacacgag cgttcgccgc tctgctcacg ctgagcgcaa tcctgtggtg cggcgccgcg
     2221 ttcgccgccg gactctccgg gctggtcttc ttctccactc agttcgcgcc ggtcgaagag
     2281 caggcgaagt ttcgcgagat tctgaaggac gggggcttcg attacactgc cgattccaac
     2341 g
//
LOCUS       DAOSWW010000398         2338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36324_length_2338_cov_3.404293, whole genome shotgun sequence.
ACCESSION   DAOSWW010000398 DAOSWW010000000
VERSION     DAOSWW010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36324_length_2338_cov_3.404293"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK442_08350"
     CDS             <1..322
                     /locus_tag="PK442_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOO87700.1"
                     /translation="KFYSLTGHVYSATMLVANDDFFASMPEDLWKIVEDAGKRYVVEQ
                     RALAEVQEQEFLEELKKTGLQINELTPEEKQLFIDATLPAYDQFKDVIGAELVELAKK
                     ANEE"
     gene            417..896
                     /locus_tag="PK442_08355"
     CDS             417..896
                     /locus_tag="PK442_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO87701.1"
                     /translation="MRKAFDRVEEVFLVGSLAFNVVLIFIQVVMRYVFQNSLSWSEEL
                     ARYIFLWQTWVGASYAVRMRRHFRVEMLVDLMKGRLRKWYELVVLLVWCAFALFIAWQ
                     GSVLTAFLIKRWQVSAAMQIPIAWAYASVPAGCAMMAIRLVMEMKDLLRDLRKGAAC"
     gene            896..2176
                     /locus_tag="PK442_08360"
     CDS             896..2176
                     /locus_tag="PK442_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO87702.1"
                     /translation="METLLLFALLIVLIATSIPIGIALGLATAIMLHFTTNVSLIMIA
                     QKSFSSLDSFPLLAIPFFILAGALMSYGGISRRLVALAESLVGFIIGGLAMVTVLACM
                     FFAAISGSGPATVSAIGSFMIPAMKERNYDAGFASALTAAAGTIGVIIPPSIPFVLYS
                     IVSGTSVGDLFLAGVVPGVIIGVLLMLNCYVIARKKGYMGDGKVHTLADAGKAFREAF
                     WALLVPVIILGGIYGGVFTPTEAAVVAVVYSVFIGIFVYKELTFASLYEALKDAVLVN
                     GATSFMVGFSMAFAAYLAMAQIPAKLGTWLAGVSTDTIVIMLLLNFILLVIGCFVDNI
                     AAVIILTPILLPVVTRLGIDPVQFGVIITVNLAIGFVTPPYGINLFVASAVSGESIEK
                     ISKNIMPFVLSMGFALLLITFIPSLSLGLVRLLR"
BASE COUNT          391 a          757 c          704 g          486 t
ORIGIN      
        1 gaaattctac tcgctcaccg gacacgtcta ctccgccacg atgctcgtgg ccaacgacga
       61 cttcttcgcg tcgatgcccg aggatctctg gaagatcgtc gaggacgcgg gcaagcgcta
      121 cgtcgtcgag cagcgggcgc tcgcggaggt gcaggagcag gagttcctcg aagagctcaa
      181 aaagacggga ctccagatca acgagctgac gcccgaagag aaacagctct tcatcgacgc
      241 cacgcttccc gcctacgacc agttcaagga cgtcatcggc gcggagctcg tcgagctggc
      301 caagaaagcc aacgaagagt aggggagcat ccgttcacag aacaggagaa tcggtgtttt
      361 cgcgccgcaa gggagcagca gccccttgcg gtgcgctgta aagaggaggg agaagagtgc
      421 gaaaagcctt cgacagagtc gaggaagtct ttcttgtagg cagtcttgcc ttcaacgtgg
      481 tccttatctt tatccaggtg gtcatgcggt acgtgttcca gaactcgctt tcgtggagcg
      541 aggagcttgc gcgctacatc tttctctggc agacgtgggt gggggccagc tacgccgtca
      601 gaatgcggcg tcacttccgc gtggagatgc tcgtcgacct tatgaagggg cgcctccgca
      661 agtggtacga gctcgtcgtg ctgctggtgt ggtgcgcgtt cgcgctcttc atcgcctggc
      721 aggggagcgt ccttacggcg tttcttatca agcgctggca ggtgtcggcg gccatgcaga
      781 ttcccatcgc gtgggcgtac gcctcggtgc ccgccgggtg cgccatgatg gcgatccgcc
      841 tcgtcatgga gatgaaggat ctgctccgcg acctgcgaaa gggagcggcc tgctgatgga
      901 gacgctgctg ctcttcgcgc ttctgatcgt cctcatcgcc accagcatcc ccatcggcat
      961 cgcgctgggc ctggccacgg cgatcatgct ccacttcacc accaacgtct cgctgatcat
     1021 gatcgcccag aagtcgttct cgtcgctcga ctccttcccg ctgctcgcca tcccgttctt
     1081 catcctcgcc ggggcgctga tgagctacgg cggcatctcg cggaggctcg tggcgctcgc
     1141 cgaaagcctc gtcgggttca tcatcggggg gctcgccatg gtcacggtgc tcgcctgcat
     1201 gttcttcgcc gccatctccg gctcgggacc ggcgacggtc tccgccatcg gctccttcat
     1261 gatccccgcg atgaaagagc gcaactacga cgcgggcttc gcctccgcgc tcaccgccgc
     1321 ggccgggacc atcggcgtca tcattccgcc gagcattccg ttcgtcctct acagcatcgt
     1381 ctcgggaacc tcggtgggcg atctcttcct tgcgggcgtc gttccggggg tgatcatcgg
     1441 cgtgctgctc atgctcaact gctacgtcat cgcgcggaag aaggggtaca tgggcgacgg
     1501 gaaggttcac acgctcgccg acgcggggaa agcgttccgc gaggccttct gggcgctgct
     1561 cgtgccggtc atcatcctcg gcggcatcta cggcggcgtt ttcaccccga ccgaggccgc
     1621 cgtcgtcgcc gtggtctact cggtcttcat cggcatcttc gtttacaagg agctcacctt
     1681 cgcgtcgctt tacgaggcgt tgaaggacgc cgtgctcgtc aacggcgcca catccttcat
     1741 ggtggggttc tccatggcgt tcgccgccta cctcgccatg gcgcagatac cggcgaagct
     1801 gggaacgtgg ctcgccggag tctccacgga caccatcgtc atcatgctgc tgctgaactt
     1861 catcctcctc gtgatcggct gcttcgtgga caacatcgcc gccgtcatca tcctgacgcc
     1921 tattctcctg ccggtggtca cgcgcctcgg catcgacccc gtccagttcg gcgtcatcat
     1981 caccgtgaac ctcgcgatcg gcttcgtcac gcccccctac gggatcaatc tcttcgtggc
     2041 gtcggccgtc tcgggagaga gcatagaaaa gatctccaaa aacatcatgc cgttcgtcct
     2101 ctcgatggga ttcgccctgc tgctgatcac gttcataccg tcgctgagcc tcggcctggt
     2161 gcggttgctt cggtagaaag gagagaacgt catgtggcct gaacatattc cctacgtgct
     2221 cgaaacggga gcggggccgg aatttccccg catgttccgc gtggaacagc tctttccccg
     2281 aaaacgcctc gacgacgtcc ccgccgccgc gaccgcctcg atggaggcgc tggcgctt
//
LOCUS       DAOSWW010000399         2333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36449_length_2333_cov_3.088235, whole genome shotgun sequence.
ACCESSION   DAOSWW010000399 DAOSWW010000000
VERSION     DAOSWW010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36449_length_2333_cov_3.088235"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PK442_08365"
     CDS             complement(<1..616)
                     /locus_tag="PK442_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87703.1"
                     /translation="MKIVIDSITKDFGSFDDPGSIFRAVDAVSLEVRDGELVTLLGPS
                     GCGKTTLLRMLAGFEDPTSGNIFFGERRMNDIAPNKRNATMVFQSYAIFPHLNVRDNI
                     AFGLKLKDLSKKEIRERTERVVELVGLEGLEERQPSQLSGGQQQRVALARAVVMEPSV
                     LLFDEPLSNLDAKLREQMRIDIRKLQQRLGITSIYVTHDQIEAMS"
     gene            complement(621..2312)
                     /locus_tag="PK442_08370"
     CDS             complement(621..2312)
                     /locus_tag="PK442_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO87704.1"
                     /translation="MTTFARGRREIQLLTRDPLLALLVVLLFVSLGVFVVFPLLSVLV
                     KSFQSEQGAFSLENYTRFLTFNYLRSSLWNSLTVGFCTAACSVLVGFAAAFTITRTSV
                     PWKKALHLLFILPIISPPFTSALSILMLFGANGLITRGLLGIRNYNIYGFKGVLLSQI
                     FTFAPVAYLTLKGVMESLNPTLEDAAMNVGAGRLQTFLRVTLPLSLPGIASAFLVVLI
                     ESLADFGNPLVLAGSRFPMLSTQAYLEITGSFNLPLGAALAVVLLIPSITSFAIQRYY
                     LQKRQYTTVTGKPVASSSKLVSRNARRGLLAFSAAFCALVLLFYGTIFVGAFTRVWGF
                     DFTPTLNHFAYAFSVGMGTIRDTLIVAICATPLSGVLGMLIAFLVVRTSFPGKGVMEF
                     TSILNFAVPGTVIGIGYILAFNTPPLMLIGTLAILVLNFVFRYIPVGIQSGIAVLRQI
                     DPSIEEAAQNLGADKLTTFRKVTLPIIAPAFFSELVFAFVRAMTAISAAIFLVSANWN
                     LLTVQILNQVGSGRLGVAAAFSVILVVIVTAAMAIIARLVPGRAGGMAAIQIQEE"
BASE COUNT          467 a          678 c          807 g          381 t
ORIGIN      
        1 tgctcattgc ctcgatctgg tcgtgcgtca cgtagatgct cgtgatgccg aggcgctgct
       61 gcaacttcct gatgtcgatg cgcatctgct cgcggagctt cgcgtcgaga ttcgagagcg
      121 gttcgtcgaa gaggaggacg gacggctcca tcacgacggc gcgcgccagc gccacccgct
      181 gctgctgtcc tcccgagagc tggctcggct ggcgttcctc cagcccctcc agcccgacga
      241 gctccacgac gcgttcggtc cgctcgcgga tctccttctt gctcagatcc ttcagcttca
      301 ggccgaaggc gatgttgtcg cgcacgttga ggtgcgggaa gatggcgtac gactggaaga
      361 ccatcgtcgc gttccgcttg ttcggcgcga tgtcgttcat ccgccgctcc ccgaagaaga
      421 tgttccccga ggtaggatcc tcgaagcccg cgagcatccg gaggagcgtt gttttgccgc
      481 atcccgaggg accgagaaga gtgaccagct cgccgtcgcg gacctccagc gagacggcgt
      541 ccaccgcccg gaagatgctg cccggatcgt cgaagctgcc gaaatccttg gttatagagt
      601 cgatgactat tttcacgagc ctattcctcc tgaatctgga ttgccgccat gccgcccgcg
      661 cggccgggga cgaggcgtgc gatgattgcc atcgccgccg tcacgatcac cacgagaatc
      721 acgctgaatg ccgcagccac gccgagacgc cccgatccca cctggttcag tatctgcacc
      781 gtgagcaggt tccagttggc cgagacgagg aagatggcgg cgctgatggc ggtcatcgcg
      841 cggacgaagg cgaagacgag ctccgagaag aaggccgggg cgatgatcgg cagcgtgacc
      901 ttccggaagg tggtcagctt gtccgcgccg aggttctgcg cggcctcctc gatggacggg
      961 tcgatctggc ggagcaccgc gattcccgac tggatgccga cggggatgta gcggaagacg
     1021 aagttcagca ccaggatcgc gagcgtgccg atcagcatga ggggcggcgt attaaaggcg
     1081 aggatgtacc cgatgccgat cacggtgccg gggacggcga agttgaggat ggaggtgaac
     1141 tccatgaccc ccttgccggg gaacgaggtg cgcaccacga ggaaggcgat gagcatcccc
     1201 agcaccccgg agagcggcgt ggcgcagatc gcgacgatga gcgtgtcgcg gatggtcccc
     1261 atgccgacgc tgaaggcgta ggcgaagtgg ttcagcgtcg gggtgaagtc gaacccccag
     1321 acgcgggtga acgcgccgac gaagatcgtc ccgtagaaga ggaggacgag ggcgcagaag
     1381 gcggcggaga aggcgaggag cccgcgtcgc gcgtttctgc tcaccagttt ggaggacgat
     1441 gcgacaggct ttccagttac ggtggtgtac tggcgcttct gcaggtagta gcgctggatg
     1501 gcgaaggagg tgatcgacgg gatcagcagc accaccgcga gagcggcccc gagcggcagg
     1561 ttgaacgagc cggtgatctc gaggtaggcc tgggtggaga gcatggggaa gcggcttccg
     1621 gcgagcacca gcgggttgcc gaagtcggcg agcgactcta tgagcaccac aaggaaggcg
     1681 ctcgcgattc ccggcagact cagcggcagc gtcacgcgca gaaaggtctg gagcctcccc
     1741 gcgcccacat tcatcgccgc gtcctccagc gtcggattca gcgactccat gacgcccttc
     1801 agcgtgaggt aggccaccgg ggcgaaggtg aagatctgcg acagcagcac tcccttgaag
     1861 ccgtagatgt tgtagtttcg gattcccagc agcccccgag tgatcaggcc gttcgcgccg
     1921 aagagcatca ggatggagag cgcgctcgtg aaggggggcg agatgatggg gaggatgaag
     1981 agcagatgga gcgccttctt ccaggggacg gacgtgcgtg tgatcgtgaa cgcagccgcg
     2041 aagccgacga gcacgctgca cgcggcggtg cagaagccga cggtgagact gttccagaga
     2101 gacgagcgaa ggtagttgaa cgtgaggaaa cgcgtatagt tctcgagcga aaacgcgccc
     2161 tgctccgact gaaagctctt gacgagcacg gagagcagcg ggaagacgac gaagacgccc
     2221 agcgacacga agagcaacac taccagaagc gcgagaaggg ggtcgcgggt cagaagctga
     2281 atctcccttc tcccgcgtgc gaatgtggtc attctgttga tcgacacctc cta
//
LOCUS       DAOSWW010000400         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36633_length_2325_cov_3.645374, whole genome shotgun sequence.
ACCESSION   DAOSWW010000400 DAOSWW010000000
VERSION     DAOSWW010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36633_length_2325_cov_3.645374"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PK442_08375"
     CDS             complement(<1..526)
                     /locus_tag="PK442_08375"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOO87705.1"
                     /translation="MTVRYPRLLVSRNRIFENAARVSERCARAGIALTGVTKGVCARE
                     ALVRAMAEGGCDELGDSRIDNLAAIRTMNVGLPLLLLRIPMPSEIAATVRIADRSLVS
                     MAQTISLIDRECRAAGRTHEIILMTDLGDLREGVWMGRVDEIAPVLKASPRVRCVGVG
                     VNFGCFGGVLPETAE"
     gene            complement(529..1371)
                     /locus_tag="PK442_08380"
     CDS             complement(529..1371)
                     /locus_tag="PK442_08380"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOO87706.1"
                     /translation="MAIIGAHVSIAGGLEKSIERGEELGCEAIQIFTKNQLQWRSAPL
                     SQSRGERFYRSWAASSIEEIVVHASYLINLAATDATGAKSIAALEDEIERCDLLGIDN
                     LVLHPGSSRGRDVESALDLVAERIASILDRTAGKRVRILLETMAGQGDLLGRNFKQFR
                     RILDALDWPSRVGICMDTCHMFAAGFDMRTEAAYQRLLDQVDDTVGLDRVGCWHFNDS
                     IHDMGRGLDRHAHIGEGTLGYAPFSFVLNDPLWEHTPCILETPQNASGHAGNMSILRK
                     LRGG"
     gene            complement(1388..2029)
                     /locus_tag="PK442_08385"
     CDS             complement(1388..2029)
                     /locus_tag="PK442_08385"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOO87707.1"
                     /translation="MKERGGERCFSPERSGKGLLMNHPVGVSFPSWEELPSGDMRQSA
                     VLVPLFPSGDELRILFLRRSPHLSRHGGEICFPGGMKDADDLSPLHTALRETEEETGI
                     SREAVEPLLLLPPEYTVVSNVEVFPVLGIVSGVDPERGLALSPSEIAASYSVDIALFP
                     AEPRRKEILFPSFTKGMRESREHTVSYPEYDLPDGSVVWGVTARILEKVCRLL"
     gene            complement(2026..>2325)
                     /locus_tag="PK442_08390"
     CDS             complement(2026..>2325)
                     /locus_tag="PK442_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOO87708.1"
                     /translation="RGAVCVARSAAEAREKMTKGAVLVARNTDKEYLPAMRLASAVVV
                     EEGGLTSHTAIVALELGIPCIVSAEGALSLLSDGMLVTVDGYRGILYHGRVKLTA"
BASE COUNT          465 a          747 c          673 g          440 t
ORIGIN      
        1 cttcggctgt ttcggggagc acccccccga aacagccgaa gttcactccg acgccgacac
       61 agcgaacacg cggagatgct ttcagcaccg gggcgatttc atcgacgcgc cccatccaca
      121 ctccctcgcg gaggtccccg aggtcggtca tgaggataat ctcgtgcgtt cgtcccgcag
      181 cgcggcactc ccggtcgatc agggagatcg tctgcgccat cgagacaaga ctcctgtccg
      241 cgatccggac agttgcggcg atttcgctgg gcatgggaat ccggagaagg agcagcggaa
      301 gtccgacgtt cattgtacgg atggcggcca gattgtcaat cctgctgtcg ccgagttcgt
      361 cgcagccgcc ctccgccatc gcccgcacga gagcctcgcg cgcgcatacg ccctttgtca
      421 cgccggtgag ggcgatcccc gcgcgagcgc atcgttcaga gaccctcgct gcgttttcaa
      481 agatccgatt cctggagacg agaagccgcg gatagcggac tgtcattctt atcctcctct
      541 cagtttacgc agaatagaca tatttccggc atggccggag gcgttctggg gcgtttcgag
      601 aatacagggc gtgtgttccc agagaggatc gttgaggacg aacgaaaacg gagcgtatcc
      661 gagagtgcct tctccgatgt gcgcgtgcct gtcgagccct ctccccatat catggatgct
      721 gtcgttaaaa tgccagcacc cgaccctgtc gagccccacg gtatcgtcca cctgatcgag
      781 caggcgctgg tacgccgctt cggtgcgcat gtcgaatccg gccgcgaaca tgtggcaggt
      841 atccatgcaa atacccacgc gcgacggcca gtccagcgcg tcgagaattc tcctgaactg
      901 cttgaaattc ctgccgagga gatctccctg acctgccatg gtctcaagaa gaatacgaac
      961 gcgtttcccc gccgtcctgt caagaataga agcgattcgc tccgccacca ggtcaagggc
     1021 tgattctacg tctcttccgc gcgacgaccc ggggtgaagg acgaggttgt cgatccccag
     1081 caggtcgcag cgctctattt catcctccag tgcggcgatg ctcttcgctc cggtggcgtc
     1141 cgtcgccgcc aggttgatga ggtacgaagc gtggacgacg atctcttcga tagaactcgc
     1201 ggcccaggag cggtaaaacc tctcgcccct ggattgcgag agaggggcgc tccgccattg
     1261 aagctggttc ttcgtgaaaa tctgtatagc ttcacatccc agctcctcgc cgcgctcgat
     1321 gctcttttca agccctccgg cgatggagac atgggcgcct attatcgcca ttgatcttct
     1381 cccttcttca cagcaggcga cacacctttt ccaatatcct cgcggtaacc ccccagacga
     1441 ccgagccgtc gggaagatcg tactccggat aggaaaccgt gtgttctctg ctttctctca
     1501 tccccttcgt gaacgaagga aaaagaattt cttttctccg gggctccgcc ggaaaaaggg
     1561 cgatgtccac cgaatacgag gcggcaatct ccgagggaga gagcgcaagc ccccgctccg
     1621 gatcgacgcc ggagacaatg ccgagcaccg gaaagacttc cacattgctg accacggtgt
     1681 actcgggggg cagcagaaga agcggctcga ccgcctcccg ggaaatgccg gtctcctcct
     1741 cggtttcgcg gagcgcagtg tgcaacggcg ataggtcgtc cgcgtccttc atcccgccgg
     1801 gaaagcagat ctctcccccg tggcgtgaaa gatggggcga gcgacgcaaa aaaagaatgc
     1861 gaagctcatc cccggaaggg aagagcggaa cgaggacggc gctctgccgc atgtccccgg
     1921 acggaagctc ctcccacgaa gggaacgaaa cgccgacggg gtgattcatc aacaatcctt
     1981 tcccggaacg ttccggggag aagcatcgct cgccgccccg ctccttcatg ccgtcagctt
     2041 cacccggccg tggtagagaa tacccctgta cccgtcgaca gtgacgagca ttccgtcgga
     2101 gagaagggag agggctccct ccgcgctgac gatgcacgga attccaagct ccagcgccac
     2161 gatcgccgtg tgggaggtaa gccccccctc ttcgacgaca acagcggagg cgagccgcat
     2221 ggccggaaga tactctttgt cggtattcct ggcgacgagc acggcgcctt tggtcatctt
     2281 ctccctggcc tcggcggcgc tccgagccac acagacggcg cccct
//
LOCUS       DAOSWW010000401         2323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36688_length_2323_cov_4.138448, whole genome shotgun sequence.
ACCESSION   DAOSWW010000401 DAOSWW010000000
VERSION     DAOSWW010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36688_length_2323_cov_4.138448"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..2034
                     /locus_tag="PK442_08395"
     CDS             190..2034
                     /locus_tag="PK442_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOO87709.1"
                     /translation="MTEPINFNALFLGPKAENYDFFKQMLVYLMDDHAEWRRFFHPED
                     PLVVTEEEKARPDFARTLQNTREALIELAGNLQVSSNPWFSPRYLGHMNADTLIAANL
                     GYMLTLLYNPNNCAYEGSPATTALEIETGRQLAALMGYDPLTAWGHITSGGTVANYEG
                     VWLARNIASIPHAIRDCMPELVEGLDDWRLMNLPVKDVLDLVDRARERGRFDEVRDRS
                     VRGTGMAGRDLGVLLVPESKHYSWAKAADIFGIGRENLIPVPVRGDYRMDVRALESII
                     DSLAAQKRPVLAVVAVVGSTEEGAVDEVHEIVRLRERYAEKGVFFYLHVDAAYGGYAR
                     ALFLDEENRFMEYDEIKKTHADVGHGGWLVPEVYEAFKAMSSADSITVDPHKLGYIPY
                     SAGAIVAADRRVIDLISYFAAYVFEKGDDNPMLLGSYILEGSKPGAAVAAVWMAHRVV
                     PLNANGYGRIIESSIEGAFRFHQSILDSTPLEVAGRRFEALPLTRPDLNIVVYAFHEI
                     GNGSLEEMNRLNQAIYEQCSYKSGPLYISDFITSKTSLTEEEYGDAPRSFVERFGIEE
                     REWRSCGSVYVLRSCILTPYLVNNTTYEIYWGNFMKSMERAIRRLYES"
     gene            complement(2031..>2323)
                     /locus_tag="PK442_08400"
     CDS             complement(2031..>2323)
                     /locus_tag="PK442_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87710.1"
                     /translation="RRSVAAGAILGASAGWMLLRYGKARMKETAERMKLPALLAAFMI
                     DEKRIADLENDLSDALADHLERRFVPLKRELDERIRTLVRDEIRALNEINSF"
BASE COUNT          456 a          704 c          742 g          421 t
ORIGIN      
        1 atctatgata ctcgaaaagc ctgttgggtc aaacaaagaa acgatcttcg tgcaggacac
       61 gctcttgact ttgacacggc gttacggtgt atcatctcgg tacggaacgg agaggagcct
      121 gcacctggct ggacgaatga gccggcgagt acaggcgcgt acctctcccg cggaaaggat
      181 ggcgaatcga tgaccgagcc tatcaatttc aatgcgctgt ttctggggcc caaggccgaa
      241 aactacgact tcttcaagca gatgctcgtc tacctgatgg acgatcatgc ggagtggcgg
      301 cgctttttcc accccgaaga tccccttgtg gtcacggaag aagaaaaggc gcggccggac
      361 ttcgcgagga cgctccagaa tacgcgagag gcgctcatcg agctggcggg aaaccttcag
      421 gtcagctcca acccgtggtt ttcgccgcgc tatctggggc atatgaacgc cgacacgctc
      481 attgcggcca accttgggta catgctcacg cttctgtaca acccgaacaa ctgcgcctac
      541 gagggctccc cggcgacgac cgcgctggag atcgaaacgg ggcgtcagct cgccgcgctg
      601 atggggtacg atccgctgac ggcgtggggg catatcacct cgggcggcac ggtcgccaac
      661 tacgaagggg tctggctggc gcgcaatatc gcctccattc cgcacgcgat ccgcgactgc
      721 atgcctgagc ttgtcgaggg cctggacgat tggcgcctca tgaacctgcc cgtgaaggac
      781 gtgctcgacc ttgtggacag ggccagggag cgcggccgct tcgacgaggt gcgcgacagg
      841 tccgtccggg ggacggggat ggccgggcgc gacctcggcg ttctgctggt tcccgagtcg
      901 aagcactact cgtgggccaa ggcggcggat atcttcggca tcggccggga gaacctgatt
      961 cccgttccgg tacgcggcga ctaccggatg gatgtccgcg ccctcgaatc catcatcgac
     1021 tcactggccg cgcaaaaacg ccccgtcctc gccgtggtgg cggtcgtcgg cagcaccgag
     1081 gagggagcgg tggacgaggt gcacgaaatc gtccggctgc gcgaacggta cgccgaaaaa
     1141 ggcgttttct tctacctgca cgtggacgcc gcatacggcg gctacgcacg ggcgctcttt
     1201 ctcgacgagg agaatcggtt tatggagtac gacgagataa agaagacgca tgcggacgtg
     1261 gggcacggag gctggctggt tccggaagtg tacgaggcgt tcaaggcaat gagcagcgcc
     1321 gactcgatca cggtcgatcc gcacaaactg ggctacatcc cgtactccgc gggcgcgatc
     1381 gtcgccgccg accggcgggt tatcgacctg atctcctact tcgccgcata cgtcttcgag
     1441 aagggggacg acaacccgat gctcctaggg agctatatcc tggaggggtc caagccgggc
     1501 gcggcggtgg cggcggtctg gatggcgcac cgggtggtgc ctctgaacgc gaacggttac
     1561 gggaggatta tcgagagcag catcgagggc gccttccgct tccatcaatc aattctcgac
     1621 agcacgccgc tggaggtcgc cggccgtcgt ttcgaggcgc ttccgctgac ccgtcccgat
     1681 ctcaacatcg tcgtctacgc cttccatgag atcggcaacg gcagcctgga ggagatgaat
     1741 cgtctgaacc aggcgatcta cgagcagtgc tcctacaaat cgggaccgct ctacatcagc
     1801 gacttcatca cgtcgaagac ctcgctgacg gaagaagagt acggggacgc gccgcgctcc
     1861 ttcgtggagc gcttcggcat agaggagagg gagtggagga gctgcggaag cgtctacgtt
     1921 ctccgctcgt gcatcctcac cccctacctg gtgaacaaca ccacctacga gatttactgg
     1981 gggaacttta tgaagtcgat ggagcgggcg atacggcggc tctacgagag ctaaaagctg
     2041 ttgatctcgt tgagcgccct gatctcgtcc cgcacgagcg tccgaatgcg ttcgtcgagc
     2101 tcccgtttca gcgggacgaa gcggcgctcc aggtgatcgg ccagcgcgtc ggacaggtca
     2161 ttctccagat ccgcgatgcg cttttcgtcg atcatgaagg cagcaaggag agccggcagc
     2221 ttcatccgtt ccgccgtctc cttcatcctc gccttgccgt agcgaagcag catccaccct
     2281 gcggaggccc ccagaatcgc gcccgccgcg acggagcgcc ggg
//
LOCUS       DAOSWW010000402         2321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36731_length_2321_cov_3.859223, whole genome shotgun sequence.
ACCESSION   DAOSWW010000402 DAOSWW010000000
VERSION     DAOSWW010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36731_length_2321_cov_3.859223"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /gene="rho"
                     /locus_tag="PK442_08405"
     CDS             complement(<1..1102)
                     /gene="rho"
                     /locus_tag="PK442_08405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOO87711.1"
                     /translation="MMSDNLIANDAADTASGTAVVETALAPAETDDASKTPPKPRHTY
                     GHLAALLNPDLRRIAKELGIVGFSSLRKDDLVIAILKAQAEELNYRFGGGTLEILQEG
                     YGFLRPKGLLPSDHDVYVSASQVRRFGLRNGDVIWGLVRPPRDSEHYEALLRVEMVNF
                     SDPEAARKRPHFGAITPIFPDEKLSLETDPKEISTRLVDLFAPIGKGQRALLVSPPKA
                     GKTTLLKKIANAVTVNYPDIILMVLLIDERPEEVTDMARSVDGEIIASTFDRPADEHL
                     RVANLALEKAKRLVEVGKDVVLLLDSITRLARASNLVVPPSGRTLSGGMDPAALYFPK
                     RFFGAARNIEEGGSLTIIGTALVDTGSRMDEVI"
     gene            complement(1294..1728)
                     /locus_tag="PK442_08410"
     CDS             complement(1294..1728)
                     /locus_tag="PK442_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87712.1"
                     /translation="MSRDGKFFLLLVGFAIFSLIAVSFGAIRSKKAAGSFEIHNIVLC
                     DEVDGGIKPVGVGSAFSYGTRQLCLWFEYSRAGDNENIRVRWYYRGRPIHSQEVVLDP
                     GSGAKAFCLLLEDGSPLPKGSYSVRISLFDRVFSDTFFEISE"
     gene            1969..>2321
                     /locus_tag="PK442_08415"
     CDS             1969..>2321
                     /locus_tag="PK442_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOO87713.1"
                     /translation="MNTTRSKEVALKGLLIAAVTLATMLSMPVPGFRLYFNFGEGIIY
                     LAALLLGPVWGAAAGGIGAAMADLLLGYPLWAPFTLLIKGTEGYIVGRIGKQHPVLGI
                     AAGAAVMIAGYSTVAG"
BASE COUNT          580 a          660 c          617 g          464 t
ORIGIN      
        1 agatcacctc gtccatccgg ctgccggtat ccaccaaagc ggtgccgatg atggtgaggc
       61 ttccgccctc ctcaatattc ctggccgctc cgaagaagcg cttcggaaag tagagcgccg
      121 caggatccat accgcctgag agcgttcgtc cggaaggagg aacaacaagg ttggatgcgc
      181 gggcgaggcg tgtgatcgaa tccagaagga gaacgacatc cttgcccacc tcgacaaggc
      241 gcttggcctt ctcgagagcg agattcgcca ctctgagatg ctcgtccgcc ggacggtcga
      301 aggtcgaggc gatgatctcc ccgtcgactg aacgtgccat atccgtcact tcttccggac
      361 gttcgtcgat aagcagcacc atcagaatga tatccggata gttcaccgtg actgcgttgg
      421 caattttctt gagcagcgtg gtcttccccg ctttgggagg agaaaccagc aacgccctct
      481 gcccctttcc aataggagcg aagaggtcga caagccgcgt ggatatctct ttcggatccg
      541 tctcaaggga aagtttttca tccgggaaaa tcggcgtgat ggcgccgaaa tgcggtcttt
      601 ttcttgccgc ctccgggtcg gagaagttga ccatctcaac tctgagcaac gcctcatagt
      661 gctccgaatc acggggagga cgcacgagac cccatataac gtctccattc cgcaagccaa
      721 aacgccgaac ctgcgacgcc gaaacgtaca cgtcgtgatc gctgggcagc aggcctttcg
      781 gacgaaggaa accgtaccct tcctgcagaa tttcaagggt tcctccgcca aaacggtagt
      841 tgagctcttc agcttgggcc ttcaggatgg cgatgacaag atcatccttg cgtagggagg
      901 aaaaaccgac tatgcccagc tccttcgcta tcctgcgaag atctggattg agaagagcgg
      961 ccagatggcc gtatgtgtgc ctcggtttcg gaggagtctt cgacgcgtca tcagtctccg
     1021 caggagcaag cgccgtttcc acaacagcgg tcccggaggc agtgtccgcc gcgtcgttcg
     1081 cgatgagatt atcggacatc atgaacgcag tcctaagcgt atttctttta taaagccagg
     1141 agggcttttt gacacacttc gggataaatg atcagagaaa aacacgaacc tggacatccc
     1201 cttcctgaat tgccccgttt caaaagcttc acgcggaaaa gaacacatga accggcgaaa
     1261 acggaccaag tttgaacttg tgaatagttg cagctactcg gaaatttcga agaacgtatc
     1321 ggaaaacact ctgtcgaaca gagaaatccg gaccgaatat gatcctttgg ggagaggaga
     1381 gccatcctca agaagcaggc agaacgcctt ggctccgctt cctggatcga gcacgacctc
     1441 ctgcgaatga atcggccgcc ccctgtaata ccagcgcacc cggatattct cgttgtcgcc
     1501 ggctctggag tactcaaacc ataaacataa ttggcgcgtg ccataagaaa aagcagaccc
     1561 tacaccgaca ggctttattc cgccgtccac ctcgtcgcat aaaacgatat tatggatttc
     1621 gaaagaaccc gcagccttct ttgaacgaat cgccccgaac gagacggcga tcagggagaa
     1681 aatagcgaaa ccaaccagaa gaagaaaaaa tttcccgtcc ctggacatta tacccaccgc
     1741 cccagaaaaa aaacaagtgc agagaaaaaa aacatcgccg ggtttcgact tccccgatcg
     1801 tttgaacagt ccagaatcag atggaaacag cgtccagctg gctctttccg aaagaactct
     1861 accacgaata gaatggggag gcaagcagga aatgtcgggt gtatctccgg atcggcaaaa
     1921 atcccgctcc cggcggacag caatgtatcc ccaaggaggt tcaatcggat gaatacaaca
     1981 cgctcaaaag aagtcgccct caaagggctc ctcatcgccg cggtcaccct tgcgacaatg
     2041 ctcagcatgc ccgtacccgg attccggctc tacttcaatt tcggagaggg cattatctat
     2101 cttgcagcgc ttcttctcgg tcctgtatgg ggcgcggcgg cagggggaat cggcgccgcg
     2161 atggccgacc tgctgctcgg ctacccgctc tgggcgccct ttacactcct gatcaaagga
     2221 actgaagggt atatcgtcgg ccggatcggg aaacagcatc cggtgcttgg aatcgccgcc
     2281 ggagcggcag tcatgatcgc ggggtactca accgtcgcgg g
//
LOCUS       DAOSWW010000403         2320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36753_length_2320_cov_3.632671, whole genome shotgun sequence.
ACCESSION   DAOSWW010000403 DAOSWW010000000
VERSION     DAOSWW010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36753_length_2320_cov_3.632671"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="PK442_08420"
     CDS             complement(<1..1617)
                     /locus_tag="PK442_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO87714.1"
                     /translation="MTSFNSRFSRVHRIRKSLGIIFFFSFFYSLFSSSPVPSQGFYMV
                     GISSFSSASASARVVRYGMVELSPFFFKDENGNFTGYMPDLLRTLAENEGWRLSFINA
                     GGEELVKMLQEGEIDLLSMAPTPRLEGLFDFSKNHHYSTWYTFFTRSDSEILTFQDLE
                     GKTISMQSGFYALSELRRILDGIGIRYTIAEAPTVEEALEMLRSGVADVCAAEQLASL
                     AKIRRFDFRRSPIIFAPSKIFFTTTKGKNTALLARLDEKLSELNESPVSLMEVLLRRW
                     YYDDEFTFFPQWALWSFFAASILLALLSAGFVIFYKKGRELRLQGVRLQERLDSERFL
                     SDAARMLLTEGGHEDVLRNACRKIREVIDARRILLLRSTFSSTRRRSIEICFEYASPG
                     LPLLSEHSEVRSVLISDIPRKWLIDLTRKKVVKERLYETPLKKFFSVSEGAFFSFYPL
                     FEGGRFWGALAVNIDDTMESSEPKDILLSTFSEMISAHLLRKRQERRLLRIATTDALT
                     GLPNRRHFFETLDREIARSLRRGHPLSLILC"
BASE COUNT          658 a          517 c          537 g          608 t
ORIGIN      
        1 gcagagaatc aacgataggg ggtgtccgcg gcgtaaactc cgcgcaattt cccgatccag
       61 tgtttcaaaa aagtgccgtc ggttcggcag tccggtaaga gcgtcggttg tcgcgatacg
      121 aagaagtcgc cgttcctggc gtttgcgcag gagatgggcc gagatcattt cggaaaatgt
      181 cgagaggagg atatctttcg gctcggagga ttccattgta tcgtctatgt tcacggctaa
      241 tgctccccag aaacgtcccc cttcaaaaag cggatagaaa gagaagaatg ccccttccga
      301 aacggagaag aattttttca atggagtttc gtagagccgc tcttttacaa ccttctttcg
      361 tgtaaggtca atgagccact ttctaggaat atcgcttatc aggacgctcc gcacttcaga
      421 gtgttccgaa agaagcggta gtccgggtga tgcatattca aagcatattt cgatcgaacg
      481 tcggcgtgta gaagaaaaag tgcttcggag gagaagaatt cttctggcat cgatcacttc
      541 acggattttt cggcacgcgt tgcgcagaac atcttcatgc ccgccttccg taagtagcat
      601 acgcgcggcg tcactaagaa aacgttccga atcaagtctt tcttgaagcc gcaccccctg
      661 taaacgaagt tcacgaccct ttttatagaa gataacgaat ccggctgaaa gtaatgccaa
      721 gagaattgac gctgcaaaga agctccacaa tgcccattga gggaagaagg taaactcatc
      781 gtcgtagtac catcggcgta gaaggacttc catgagagat actggagact cgttcagttc
      841 agaaagtttt tcgtcgagtc gagccaagag cgctgtgttt tttccttttg tagtggtaaa
      901 aaagattttg gagggcgcaa aaataatcgg gcttctccgg aaatcaaaac gtcgaatctt
      961 ggccaggcta gcaagctgtt ccgccgcgca tacgtcggca acgccggagc gcagcatctc
     1021 aagcgcttcc tccactgttg gcgcttccgc tatcgtgtag cggattccta taccgtccag
     1081 gattcggcgg agttcggaaa gcgcataaaa gccgctttgc attgaaatag ttttgccttc
     1141 aagatcctga aatgtcagta tctccgagtc agaacgcgtg aaaaaggtgt accacgtaga
     1201 ataatgatga ttcttagaga aatcgaaaag cccttccaaa cggggcgtag gagccatact
     1261 caaaaggtcg atttctccct cctgaagcat tttgacaagc tcttcaccgc cggcgttaat
     1321 gaaggaaagc ctccacccct cattttcagc aagggttcgc agaagatccg gcatatagcc
     1381 cgtaaagttc ccattctcat ccttgaaaaa aaacggggat aactctacca ttccgtatcg
     1441 aacaacacgc gcggaagcgg aagcagaaga aaacgaagaa atgcccacca tataaaaacc
     1501 ctgggaagga acgggggaag aagaaaaaag actgtaaaaa aaggaaaaaa agaagataat
     1561 gccaagagat ttcctgatac ggtgtactct tgaaaaacga gagttgaatg aggtcaagaa
     1621 acagacactc cctttcggag taaaacaacc ttgccatgtt ttctcaatat ttatttcgga
     1681 cccgcaatgg ttttaaaata tgtattccat gtcatcgctg tacaaagaaa gcaaattgac
     1741 tgcttgagaa tattttgata acgcatcgca cgctcatctg aaagaattat atgaagaaaa
     1801 caagagttgc gcaagcccaa ttccaggctt acgttgtcta ttaagtgggc actaaggttc
     1861 atatatataa aagtttatct cgttttttgt cgctacgtgt actaaaagta tcttttgaca
     1921 ttactttgat tgtggggtgg attcttggag tgagaacgaa aaaaaatgga agccctaata
     1981 gcggttaagc tcgattgcat caagttcctg cgagactacc tccgctttat tcagaggttc
     2041 cgtctatcat catctgtctt tccttcttga aaaccatgga ggttccaggg aggaataacg
     2101 ctctttcatt tttttcgttt tcttaaaaca tatggttgtg ggtaggactg cacagagaat
     2161 aatttactca tccgaatggc tcacttttgt caatgcttac taatatagtt tttccgcact
     2221 cattgttagc gtttttcctt ttcgcactcc cctcctgttt gaaagcaaag cttctccgtc
     2281 gaatatgttc gttttccata attctctgag gggttatctt
//
LOCUS       DAOSWW010000404         2311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_36971_length_2311_cov_3.944149, whole genome shotgun sequence.
ACCESSION   DAOSWW010000404 DAOSWW010000000
VERSION     DAOSWW010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36971_length_2311_cov_3.944149"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PK442_08425"
     CDS             complement(<1..581)
                     /locus_tag="PK442_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOO87715.1"
                     /translation="MSTVQWYFPDTLDEAISYLGREKTVPHGGGTSLIKSGIASLEGL
                     VDLSRLGLDSLKMEKGILEAGAGMTFGDVGESLANLHPECILGKALGASASTPMRNRI
                     TLGGSASLFPLWSDLIGPMSVLHGIVCTEGKNGGTYDLPSWLADRSLQKGSLITSLLF
                     PDPVDWLSFYYRETRVGFDYPAFTVSILARKNN"
     gene            complement(697..1791)
                     /locus_tag="PK442_08430"
     CDS             complement(697..1791)
                     /locus_tag="PK442_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO87716.1"
                     /translation="MEVAAVVLAAGYSSRMGFFKPLALLNGVSALERILRSMRGAGVR
                     NVLVVTGFNEDVVASAAQRAGARAVYNPRYAEGMFTSVQVGVAALPHSADAFFLLPVD
                     IPTVRVSTLRLMMESASSNGILHPLFRGKRGHPPLIAAALAPHILDFSGEGGLRAFFD
                     GWATWNQDFDVADEGVLMDMDTPQDFEKIHRRLSGLRVPSKMEREVLFDLAGTPLRVR
                     AHCDATASVCLKLAFALRQQGVRIDMPLLHGAALFHDVCRHVPDHVCAGEIFLRRHGF
                     PLLAEIAACHRDLPPLPRIEATLLHLADKLVSDTHIISLDARKKNMRMRYGADQNALL
                     AIRQRYAAARKTARRIERITGQSLETLLQI"
     gene            complement(1825..>2311)
                     /locus_tag="PK442_08435"
     CDS             complement(1825..>2311)
                     /locus_tag="PK442_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOO87717.1"
                     /translation="YHAMLSGRTSRDQRKRTHAIESYFRRVNPRLSAANASRYAGLIE
                     KYSQHYKVDPFLVAAILVKESTVKSNAVSKGNYGLMQVNWKANGPWIRKTFPVRTTKQ
                     LLEPENNIRIGVHILASNIQRAGGDVDKGLDRYRGRSLASYRNSVLRNYSAIYTLFAR
                     R"
BASE COUNT          557 a          630 c          598 g          526 t
ORIGIN      
        1 ctgttgtttt tgcgagcaag gatggagacg gtgaacgccg gataatcgaa tccgacacgg
       61 gtctccctgt aatagaagga gagccaatca accggatccg gaaagagaag cgatgtgatc
      121 agagatccct tctgaagaga tctgtccgca agccaggatg gaagatcgta cgttccgccg
      181 ttcttcccct cggtgcagac gattccatgc agcaccgaca tggggccgat aagatcggac
      241 cagagcggga agagcgaagc gctgcctcca agagtaatcc ggtttctcat cggggtagaa
      301 gcagacgctc cgagagcctt tcccaggatg cattcggggt gcagattggc aaggctctcc
      361 ccgacgtccc cgaacgtcat tcccgctccg gcttcaagga ttcctttttc catcttcagc
      421 gaatcaaggc ccaatctgga gaggtctacc aaaccttcca gggaggcgat cccgcttttg
      481 atcaaactcg ttcctccgcc gtgaggaaca gttttctccc tgccgagata cgagatagct
      541 tcatcgagtg tatccggaaa ataccattgc acagtactca tatttctcct cctttgtttt
      601 gtcatctgca cagttcatac actactctgg aggagagacg ttcctccatc cattcacttt
      661 ataacattca atgtcaactg tctagagggg ttcttcttaa atttgcagca aagtttcgag
      721 agactgtcct gttatgcgtt ctattctgcg tgcggttttt cttgcagcgg cgtatctttg
      781 gcgaatagcc agcaatgcgt tttgatctgc accatatcgc atacgcatgt tctttttcct
      841 ggcatcgaga gaaatgatgt gcgtatcgga aacgagtttg tccgcaagat gaagaagggt
      901 agcctcgatt cggggcaaag ggggcagatc cctgtgacat gctgctattt ctgcgagaag
      961 aggaaaaccg tgacgccgca aaaaaatctc gcctgcacac acatgatccg gtacgtgcct
     1021 gcacacgtcg tgaaacagcg ccgcgccatg aagcagcggc atatctatcc tgactccctg
     1081 ttgccgcaaa gcgaaagcga gcttcaggca aacagaggcg gttgcgtcgc aatgggcgcg
     1141 aacacgcaac ggcgtgcccg ccaggtcgaa gaggacctct cgttccattt tcgagggaac
     1201 gcgcagtccg ctcaaacgac ggtgaatttt ttcaaagtct tgcggcgtat ccatatccat
     1261 gagcacgccc tcgtccgcta cgtcgaagtc ttgattccac gttgcccacc catcaaaaaa
     1321 ggcgcgcaac ccgccttctc cggaaaaatc aagtatatgg ggcgccaagg cagccgcgat
     1381 aagcggagga tgtccccgct tccctcgaaa cagagggtga agaatcccat tggaagaagc
     1441 gctttccatc attaaacgca atgtggaaac ccgaactgtc ggaatatcta cgggcaggag
     1501 gaaaaaagcg tctgcactgt gcggaagcgc agctacgcct acctgtactg aagtaaacat
     1561 accctccgca taacggggat tatagacggc ccgggcgccg gcgcgttgag cagctgaggc
     1621 gacaacgtct tcattaaatc ctgtcacgac gagaacattc cgtaccccgg cgccacgcat
     1681 tgaacgaaga atccgctcca gagcagacac tccgttcagg agggcaagcg gcttgaaaaa
     1741 gcccatacgg ctggaatacc ccgccgcgag aacaaccgct gcgacttcca tcgacagatc
     1801 tcctttgttg tgttcgctac acactcaacg gcgagcaaac aaggtgtaga tcgccgaata
     1861 gttccgcagg accgaatttc tataggaggc cagcgagcgg ccccgatacc tgtcgagccc
     1921 cttatccaca tctccgccgg cacgctggat attcgatgcc agtatatgaa cgccgatgcg
     1981 tatgttgttc tcgggctcaa ggagctgttt cgtcgtacgg actggaaatg tttttcgtat
     2041 ccagggacca tttgctttcc agttgacctg catcaaccca tagttcccct tgctgacggc
     2101 attggattta acggtggatt ccttcacaag aatggcggcg accagaaacg ggtcaacctt
     2161 ataatgctgg gagtattttt ctatcaatcc ggcgtaacgg gaggcatttg ctgcggaaag
     2221 acgaggattc actcttctga agtagctttc gatcgcgtgc gttctttttc tttgatcccg
     2281 agaggtcctg ccgctcagca tcgcatggta t
//
LOCUS       DAOSWW010000405         2309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37025_length_2309_cov_4.096717, whole genome shotgun sequence.
ACCESSION   DAOSWW010000405 DAOSWW010000000
VERSION     DAOSWW010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37025_length_2309_cov_4.096717"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PK442_08440"
     CDS             complement(<1..916)
                     /locus_tag="PK442_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOO87718.1"
                     /translation="MNASQETTALLQSAREVLDENWKDGFTVPAQNQYPYQWSWDSAF
                     IAAGYARYDRERAQRELRSLFSAQWADGRVPHIVFHNATPDASYFPGPEFWQTERNPF
                     APLSPRTSGIVQPPNHATAVLHIVRCAGEEGEKAALAFAGEMFPKLRAWHDWLYRERD
                     PRGEGVVYIIHPWESGQDNSPLWDPVLARMDVPPARISGFRRRDTRAVAPEERPLDSD
                     YDRYIFLVDFFRQRGYDERKIFGDGCPFLVQDVLFNTLLCRAEQDMAELARLLGENPA
                     PFEARARRTADAVNRKLWDGDRGYYADYD"
     gene            complement(1035..2132)
                     /locus_tag="PK442_08445"
     CDS             complement(1035..2132)
                     /locus_tag="PK442_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO87719.1"
                     /translation="MSEIRIENVVKRFGDFVAVNDVSLVVAEHEFMVLLGPSGCGKTT
                     LLRAIAGLGMADEGRISIGGRDVTYLPPRERRISMVFQSYAIFPHMNVYDNIAFGLRM
                     QKEEKTEIENRVRRAAELLHIEAMLDRYPSKMSGGQRQRVAVARAIAMQAQVLLMDEP
                     LSNLDALLRLEMRAELKQLLAEIGTTTVYVTHDQIEAMSMGDRIAVMHGGTVQQVDSP
                     TTVYDYPANRFIGGFIGNPPMNFLEGQVRRKDGDTFVRLGEFDVTPTPRMQPVLKEYD
                     GKPIVLGIRAENLETLHEPAHDALRTDVLVVEPLGSQNLLTAAFGGHRIKVSTHPGFP
                     ARAGGEVWLRFPPESVRWIDPDTKRALYPSL"
BASE COUNT          388 a          743 c          729 g          449 t
ORIGIN      
        1 gatcgtagtc ggcgtagtag cccctgtcgc cgtcccacag cttcctgttc acggcgtcgg
       61 cggtgcggcg agcgcgcgct tcgaacggcg cggggttttc gcccagaaga cgggccagct
      121 ccgccatgtc ctgttccgcg cggcagagca gcgtgttgaa caggacgtcc tgcaccagaa
      181 acgggcagcc gtcgccgaag atcttccgtt cgtcgtaccc ccgctgccgg aagaagtcca
      241 caagaaagat atagcggtcg tagtccgagt cgagcggccg ttcctcggga gctacggcgc
      301 gagtgtctct tctgcggaag ccggagatcc gggcgggcgg cacgtccatc cgtgccagca
      361 cgggatccca caggggggag ttatcctggc cggactccca cgggtggatg atgtacacca
      421 caccctcgcc tctcgggtcg cgttcccggt agagccagtc gtgccatgcg cgcagcttgg
      481 ggaacatttc tcctgcgaag gcgagcgccg ccttctcccc ctcctctccc gcgcagcgga
      541 cgatgtggag gactgccgtg gcgtggttcg gcggctgcac aatcccggaa gtgcgtgggg
      601 aaagaggggc gaacgggttt cgttcggtct gccagaattc cggacccggg aagtacgatg
      661 cgtcgggggt cgcgttgtgg aagacgatgt gcggtacgcg cccgtccgcc cactgcgcgc
      721 tgaagagaga acgaagttcg cgctgcgctc gctcccggtc gtagcgggcg tacccggcgg
      781 cgatgaacgc ggagtcccag gaccactggt acgggtactg gttctgtgcg ggaacggtga
      841 atccgtcctt ccagttttcg tcgagcacct cgcgagccga ttgcagcagc gcggttgtct
      901 cctgtgaagc gttcattgta cagcctccct tgccttcata agcgctcccg gacgaagccg
      961 gccggaatcg tgcgaaacgg tgtccgcgag agcggcatgt tcgtttcact cttcgcctcg
     1021 ctgcggaaag ttcctcagag tgacggatag agcgcgcgct tcgtgtccgg atcgatccag
     1081 cggacgctct ccggagggaa gcgaagccag acttcgcctc cggcgcgcgc cggaaatccg
     1141 gggtgcgtgg aaaccttgat tctatggccg ccgaaggccg cagtcaacag attctgcgag
     1201 ccgagcggct ccacgaccag aacgtcggtc cgcagggcgt catgggccgg ttcgtgcagc
     1261 gtctccagat tctcggccct gatgcccagc acgatgggct tgccgtcgta ctccttcagg
     1321 accggctgca ttcggggcgt tggcgtcacg tcgaactctc ccaggcggac aaaagtatcg
     1381 ccgtccttcc tgcggacctg cccttcgaga aagttcatcg gcgggttccc gatgaatcct
     1441 ccgataaagc ggttcgcagg gtagtcgtac acggtcgtcg gggagtccac ctgctggacc
     1501 gttccgccgt gcatcaccgc gatgcggtcg cccatgctca ttgcctctat ctggtcgtgc
     1561 gtgacgtaga cggtggtcgt tccgatctcg gccagaagct gtttcagctc ggcgcgcatc
     1621 tccagccgga gcagcgcgtc gagattgctc aacggctcgt ccatcagcag cacctgcgcc
     1681 tgcatcgcga tggcgcgcgc caccgccacg cgctgcctct gaccgccgct catcttcgac
     1741 gggtagcggt cgagcatcgc ctcgatgtgc agcagctccg ccgcccgcct cacccggttt
     1801 tctatctccg tcttctcctc cttctgcatc cgcaggccaa aggcgatatt gtcgtagacg
     1861 ttcatatgcg ggaagatggc gtagctctga aagaccatcg agatgcgccg ttcccgcggg
     1921 ggcaggtagg tgacgtcccg cccgccgatg ctgatcctgc cttcgtccgc catgccgagc
     1981 ccggcgatgg cgcggagcag cgtcgtcttg ccgcagccgc tgggacccag cagcaccatg
     2041 aactcatgct cggcgacgac gagcgagacg tcgttcaccg ccacgaagtc gccgaacctc
     2101 ttgacgacat tttctattcg tatctccgac atgattccgc tccttccgta attttgttca
     2161 cttgctggac tgtcccgaca tgttgaaaag gtacttgcgg atgatcatga tgaagatcac
     2221 cgagggaatc aacatgaaga atcctcccgc gaacttgtac gcgtcggggg cctcccgcag
     2281 cgagacgagc acctgcgcgg gcagcgtgc
//
LOCUS       DAOSWW010000406         2307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37080_length_2307_cov_3.709591, whole genome shotgun sequence.
ACCESSION   DAOSWW010000406 DAOSWW010000000
VERSION     DAOSWW010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37080_length_2307_cov_3.709591"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PK442_08450"
     CDS             <1..377
                     /locus_tag="PK442_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOO87720.1"
                     /translation="PHCAEVGCLRTLLSIPSGERHEMCRGSHAEMNAIAQAAAAGVCT
                     AGGQIYCTHEPCSFCTKAIINAGIERVIFLHPYPDTLAREMRSQASIEVTCLSPDFFA
                     TAGALLGDLFSPAENARRNETE"
     gene            374..958
                     /locus_tag="PK442_08455"
     CDS             374..958
                     /locus_tag="PK442_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOO87721.1"
                     /translation="MNFTRENALDLLKKHNKDEGHIKHALAVESSMKFFAEKMGGDVE
                     KWGMAGLLHDIDWEEMQETPERHTIEGARWLSEEGYPEDIVRAVLAHGWGICSDVKPE
                     NDMEKVLFTIDELTGLVMTAALVRPSRSVQDLEVKSVKKKWKDKAFAKGVDRELIVKG
                     AEMIPMPLDTVIEWVILAMRRIEPDLGLGSAAGA"
     gene            1064..1699
                     /locus_tag="PK442_08460"
     CDS             1064..1699
                     /locus_tag="PK442_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO87722.1"
                     /translation="MPVRVFLADDHPLTRAGISAYLAQETSVELVGEAEDGETAWKLI
                     SEIRPDVALLDIRMPGEDGVSVARRVKESGLPVAVVMLTSYDAQQYVLASLRAGARGF
                     ILKTATPEEITRAINTVAKGGFYLDSEVASAMGDGEFTPEQLSAREREVLILASKGFS
                     SKEVAARLFISERTVQTHLASIYDKLGAKNKTEALLLALKYGVVTLEELLE"
     gene            1696..>2307
                     /locus_tag="PK442_08465"
     CDS             1696..>2307
                     /locus_tag="PK442_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOO87723.1"
                     /translation="MRRHLLLFIIFALMLPSFAVLLVSGIGILQHEWAIRSVARSYVQ
                     EMAENVASRVATSEPLGWGMELSMLGIHGFRVFTWGPSIPGWVAVLHADGRLLFSSPG
                     ADIAAVWRPNIPIGRAVEIKDKDGDLYTLAVYPVRGGERFVIAAVAWEQLLGPLARGG
                     MLWSVLIVIMAICILLALWALWRWLIRPLRSLVAELEGLSWGRD"
BASE COUNT          526 a          539 c          680 g          562 t
ORIGIN      
        1 tgccgcactg cgcggaggta ggatgtctcc gtaccttact ctctatcccg tcaggagaac
       61 gccatgaaat gtgcagagga tcccacgcgg agatgaatgc gatagctcag gcagcagctg
      121 caggcgtatg tactgcgggc gggcagatat actgcacgca tgaaccctgc tccttttgta
      181 caaaagcgat tattaacgcc ggtattgaac gggtgatttt tcttcacccg tacccagata
      241 ctcttgcaag agagatgagg tctcaggcgt ctatcgaagt gacatgcctt tccccggact
      301 tctttgctac cgccggggct cttcttgggg atcttttttc gccggcggaa aatgcgagga
      361 ggaacgaaac agaatgaatt tcacacgaga aaatgcactg gatcttttga aaaaacacaa
      421 caaggatgaa ggtcacatca agcatgccct tgccgtggaa tcctctatga aattctttgc
      481 cgagaagatg ggtggagatg tagaaaaatg gggtatggcg ggacttctgc acgacatcga
      541 ctgggaagag atgcaggaaa cgccggaacg gcataccatt gagggggcgc gctggctttc
      601 tgaagaaggg tatcccgagg atatcgtgag agcggttctt gctcacggat ggggcatctg
      661 ctcggatgtg aaacctgaaa acgatatgga aaaagtcctt ttcacgatcg acgaactcac
      721 cggtcttgtg atgaccgcgg cattggttcg cccaagccgt tcagtacagg atcttgaggt
      781 caagtcggtg aagaagaagt ggaaggataa agcctttgcg aaaggcgtgg atcgtgaact
      841 gatagtcaaa ggagccgaga tgattccaat gcctttggat accgtcatag aatgggtcat
      901 cctggccatg agacgtatcg aaccggattt ggggctcgga tcagcggcgg gagcctgaga
      961 tactgttttt tcggcgtctt tgccgatgtt ttcctctttt tgtcctgata ccatgcttta
     1021 cggacaataa aaactccctt gaccgggaaa aggagtggat agtatgcctg tacgagtctt
     1081 tcttgctgac gatcatccgc tgacgcgagc cggcatatcg gcctatcttg cacaggaaac
     1141 ctccgtggag ctggtggggg aggctgaaga cggcgagacc gcgtggaagc tcatttcgga
     1201 gatacgtcct gacgtggcgc ttttggatat caggatgccg ggtgaagacg gggtctcagt
     1261 tgcccggaga gtgaaagagt cggggcttcc tgtcgcggtt gtcatgctca cttcctacga
     1321 tgcccagcaa tacgttcttg cgtctcttcg ggcgggcgcg agaggattta tcctcaagac
     1381 cgccacgccg gaggagatta ccagggcaat caacacagtg gccaaaggcg ggttttatct
     1441 cgattcagag gttgcttccg ctatgggcga cggagaattt actccggaac agctttcggc
     1501 gagggaaaga gaagtcctca ttctggcgtc gaaaggcttt tccagcaaag aggtcgccgc
     1561 tcgtctcttt attagcgaac gaacagttca gacgcatctt gcctccattt atgacaaact
     1621 tggtgcgaaa aacaagaccg aagcgctcct gctggcgctg aaatacgggg tggtgaccct
     1681 ggaggaactg cttgaatgag aaggcacctc cttctcttca tcatttttgc tcttatgctt
     1741 ccctcgtttg cggtgctcct cgtttcagga atcggcatac tccagcacga atgggcaatt
     1801 cgttcggtag cccgttcata cgtgcaggag atggcggaaa atgttgcatc aagagttgcc
     1861 acgagcgaac ctctcggatg gggaatggaa ctgagcatgc ttgggataca cggatttcgt
     1921 gtttttacat ggggaccgtc catcccgggg tgggtagctg tacttcatgc agatggaagg
     1981 ttgctgtttt cctcgccggg agcggatatc gctgcagtat ggcgtccgaa cataccgatt
     2041 ggaagagccg tcgaaatcaa ggataaagac ggagatttgt atactcttgc cgtgtaccct
     2101 gtccggggag gagaacggtt tgtcattgcg gccgttgcct gggaacagct ccttggcccc
     2161 cttgcacgag ggggtatgct ctggtctgtt cttattgtca ttatggctat atgtattctg
     2221 ctggcgcttt gggcattgtg gagatggctt attcgcccgc tccgaagtct tgtcgccgaa
     2281 ctcgaagggc ttagttgggg acgagac
//
LOCUS       DAOSWW010000407         2299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37303_length_2299_cov_2.939840, whole genome shotgun sequence.
ACCESSION   DAOSWW010000407 DAOSWW010000000
VERSION     DAOSWW010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37303_length_2299_cov_2.939840"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PK442_08470"
     CDS             <1..413
                     /locus_tag="PK442_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87724.1"
                     /translation="ERGLSLTPTLRTAPYLHPKKFAHISTRWQISFPDGASSAAQGVH
                     LVFESTSSTELTELHVPEGVLLHGTEYVWRTRFRDSQNGWSDWSPYRRFFTADAPPED
                     KPESLSASAGGCSSVSGRGALLLLPLMALLLRRL"
     gene            580..1335
                     /locus_tag="PK442_08475"
     CDS             580..1335
                     /locus_tag="PK442_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOO87725.1"
                     /translation="MELVWYHWAGIVLVGVMQGFLNTVAGGGTLLVLPALTFLGMDLA
                     VANGTNRIAILLQSISGAASFKQQKALSFKVALPLAAAATLGALVGTFVVVQVDRKSL
                     NLVIAALITLMALLLVFKPKMWEEVKEQHWPKWGVYLAFFAVGIYGGFIQAGVGFFLS
                     WALVVAAGMDLVSGNAVKTIIIGCYTTISLAIFYMNGLVNVPVGLVLAIGSMAGAVLG
                     ARFTVMKGNKWVRYVLAFVVVVSAVKMVMDAIG"
     gene            complement(1397..1840)
                     /locus_tag="PK442_08480"
     CDS             complement(1397..1840)
                     /locus_tag="PK442_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87726.1"
                     /translation="MNAKESARQFLREVWRLIKEDTVPSMQEYLNLPEWDKIQLAGMG
                     MCLGDIYKAFEQSLRCILENIEGKKIEKNESWHSNLLRLSNEMELIPTDIRDTVQGML
                     KYRHHLHHGYGVKLNERLIRKYAPEAIDAFDAFTKHIISRYFDIL"
     gene            complement(1803..2114)
                     /locus_tag="PK442_08485"
     CDS             complement(1803..2114)
                     /locus_tag="PK442_08485"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO87727.1"
                     /translation="MLSQKSLEFIKEIGEKHGVQRIVLFGSCLHLPETEAGDIDLAVG
                     GLDAETFVEMWNDLTWASELHMKNVDLVRMEDALPVMVMVRAEGVTIYECERERATVS
                     A"
BASE COUNT          424 a          624 c          697 g          554 t
ORIGIN      
        1 gcgagcgagg cctttccctg accccgacgc tgcgcaccgc gccttatttg cacccgaaaa
       61 aattcgcgca tatctcgacg cgctggcaga tatcgtttcc tgacggcgcg tcctctgcgg
      121 cgcagggtgt gcatctcgtt ttcgaaagta cgagcagcac ggaactgacc gaactccacg
      181 ttccggaagg agtattgctt catggaacgg agtatgtttg gaggacccgc tttcgcgatt
      241 cacagaacgg ctggtcggac tggtcgccgt accgacgttt tttcactgcg gatgctccgc
      301 cggaagacaa gcctgagtct ctttccgctt ccgccggcgg gtgtagcagc gtttccggca
      361 ggggcgcgct cttgctgctc ccgctcatgg cgcttctctt gcggcgttta taggagcgct
      421 ccggcttcgt tgagcaaggg tgcacgcctc gtagaaaaaa cgcctctttt catccgttct
      481 ccgatttgat atactgcaaa aaacatgttg tgagcatgcc gagcgtacgg gagcctcgga
      541 agaggctccc tctatcattt gaagaaggtt gtgagaggta tggaactggt atggtaccac
      601 tgggcgggga tcgtcctcgt cggcgtgatg cagggctttt tgaacacggt cgcgggcggc
      661 ggcacgctgc ttgtgcttcc ggcgctcacc ttccttggga tggatttggc ggtggcgaac
      721 gggacgaacc gcatcgccat tctgttgcag agcatctcgg gggcggcgag cttcaagcag
      781 cagaaggcgc tctcctttaa agtggcgctg ccgctggccg ccgcggcgac gctgggcgcg
      841 ctcgttggga cgttcgttgt ggtacaggtg gacaggaaga gtctgaacct cgtcatcgcg
      901 gcgctgatca cgctgatggc gctgctgctg gtcttcaagc cgaagatgtg ggaagaggtg
      961 aaggagcagc actggccgaa gtggggcgtc tacctcgcgt tcttcgcggt gggtatctat
     1021 ggcggcttca tccaggcggg cgtcggcttc ttcctttcgt gggcgctcgt ggtggcggcg
     1081 gggatggacc tggtgagcgg caacgcggtg aagacgatca tcatcggctg ctacacgacg
     1141 atcagcctgg cgatcttcta catgaacggc ctggtgaacg tgcctgtggg cctggtgctc
     1201 gcgatcggga gcatggcggg cgcggttttg ggcgcgcgct tcacggtgat gaaggggaac
     1261 aagtgggtcc gctacgtcct cgcgttcgtg gtcgtcgtca gcgcggtgaa gatggtgatg
     1321 gacgcgatag ggtagaaaag aaacaaccga tagaaatgat gaagccccgg tgtcactgcc
     1381 cggggctttg ttcgattcaa aggatatcaa agtagcggct gataatatgt tttgtaaagg
     1441 catcgaaggc atcgattgcc tcgggcgcgt attttcgtat aagccgctcg ttcagcttga
     1501 cgccgtagcc gtggtgaagg tgatgcctgt acttgagcat accctgtacg gtgtcccgaa
     1561 tatctgtggg gatgagctcc atttcgttgg aaaggcgcaa caagttactg tgccaggatt
     1621 cgttcttctc gatctttttg ccttcgatat tttcgagaat acaccgcaag gactgttcga
     1681 aggctttata aatatctcca aggcacatgc ccatgccggc aagctggatt ttatcccatt
     1741 ccggcaggtt gagatactcc tgcatcgacg gcacggtgtc ttcttttata agtctccaca
     1801 cttcacgcag aaactgtcgc gcgctctctt tcgcattcat agatcgtcac tccttcggcg
     1861 cggaccatca ccatgacggg cagggcatcc tccatccgta cgaggtcgac gtttttcata
     1921 tgaagttcag aagcccacgt gaggtcgttc cacatctcga caaacgtttc cgcgtcgagt
     1981 ccgccgacgg ccaggtcgat gtcgcccgct tccgtctccg gaagatgcag gcaggaaccg
     2041 aagagcacaa tgcgttgcac tccgtgtttc tctcctattt ctttgatgaa ttcaaggctt
     2101 ttttgcgaga gcacaactct ccctccttgc aggcgacgct gaatgatttt ttcgcgtccc
     2161 catatcgttc ttattctacc atctctacag ccggtgcgcg cccgccgcga cgaaggagag
     2221 gcccgccttc gtcagggcct ccgctgtgct gttgacggcc ttgtgcttca tcaggtagtt
     2281 cagcgtgagg gcgtccatg
//
LOCUS       DAOSWW010000408         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37398_length_2296_cov_2.497992, whole genome shotgun sequence.
ACCESSION   DAOSWW010000408 DAOSWW010000000
VERSION     DAOSWW010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37398_length_2296_cov_2.497992"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1211
                     /locus_tag="PK442_08490"
     CDS             222..1211
                     /locus_tag="PK442_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOO87728.1"
                     /translation="MNKKRIAVLCVALFVLAFAGAGFASTKFVTIVTGSTGGTYYPIG
                     TILANHFNTALMEKGYKWSAQSSGGTVENLDMMQRGEAEMAIAMANITGFAYTGTQTY
                     EGKKIENLRYVMGLWPDVTQFVVSGSSGIKTWADLKGKKVAVGPAASGTEFSSKVLLQ
                     ALAGLTFDDIKAEYVGYSEAAQALQNGQLDAFNAEAGVPVGAVAELYAGRQEVNMLEF
                     SAEDLAKVKAVAPFYAGVLIPAGTYPKQEKDLRVAGIKSALLVGKDVPDEVVYDMLKI
                     IYAKKDDLLNEHAAFKKVDFDNPIDGLSGAPLHPGAVKFFSEIGLTIPAELLP"
     gene            1294..>2296
                     /locus_tag="PK442_08495"
     CDS             1294..>2296
                     /locus_tag="PK442_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HOO87729.1"
                     /translation="MFMKGFVSLFVPGESIPTRKLTGPAGAAALVLTVATALVHFWMN
                     SIGLLIAVKMNAIHLGTLMAIIFLFYPAFAGSPRERPSLPDWVLALTSLGCMFWLLFT
                     YDRLLQTNLQATFPDLAVAVITMALLVEASRRAVGLPLTMLSIFFLAYTRFGPMFPGL
                     FAHRGFNWERIIIRMALTDQGIYGVTLMVSSSYVFMFILFGAFLAASRTSEFFNDFSL
                     ALAGKYRGGPAKVAVMASALMGSISGSAQANVATTGAFTIPLMKKVGYMPHFAGAVEA
                     CASTGGQFMPPIMGAGAFIMSEFLGISYLRIAAAAIIPAMLYYSALFIAVHLRARHRN
                     "
BASE COUNT          459 a          642 c          666 g          529 t
ORIGIN      
        1 tgacgaagaa tttaatccgc tgaaatcatg attcgagttt agcgaaattg tcaattggaa
       61 aataatattc agaacaggag tctcttgaaa gccgaagaga ctcctgttac aatcagcggg
      121 tattttgata acagtaagtg ttgatagtgt ttcggggggg tgaaatcacc gaagacgtgg
      181 ggtgcggtga agtacacaat ttcgagagag gtggatgaaa aatgaacaaa aagagaatcg
      241 ctgttttgtg tgtcgcgctg tttgttctgg cctttgcagg agccggattt gcttcgacga
      301 agtttgtcac catcgtcacc ggttccacgg gcggaacgta ctatcccatc ggtacgatcc
      361 tcgccaatca tttcaacacg gcgttgatgg agaaggggta caagtggtcc gcgcagagct
      421 ccggcggcac ggtcgagaat ctcgatatga tgcagcgcgg agaggccgag atggcgatcg
      481 ccatggcgaa tataaccggc ttcgcctaca ccgggacgca gacctacgaa gggaagaaga
      541 tcgaaaacct ccggtatgtc atggggctgt ggccggacgt gacgcagttc gtcgtcagcg
      601 gctcctccgg cattaaaacc tgggccgatc tcaagggcaa gaaggtcgcc gtgggacctg
      661 ccgcctccgg cacggagttc agcagcaagg tgcttcttca ggcccttgcc ggtttgacct
      721 tcgacgatat caaggccgag tacgtgggat acagcgaggc agcccaggcg cttcagaacg
      781 ggcagctcga cgcgttcaac gccgaggccg gcgtccctgt gggcgcggtt gccgaactct
      841 atgcgggccg ccaggaagtg aacatgcttg agttctctgc ggaggatctg gcgaaggtaa
      901 aggcagtcgc tcccttctat gcaggcgtgc ttatcccggc cggaacctat ccgaagcagg
      961 agaaggatct ccgggtcgcc ggcatcaagt cggcgctgct tgtcggaaag gacgttcccg
     1021 atgaagtcgt ctacgacatg ctgaagatca tctacgcgaa aaaggatgat cttttgaatg
     1081 agcatgccgc cttcaaaaag gtcgattttg ataacccgat cgacggtctt tcaggggcgc
     1141 ctctgcatcc gggcgcggtg aaattcttct cggagatcgg cctgaccatt ccggcggagc
     1201 ttctcccgta gtctcttttc cgtctgttct ttattcgacg cgggcggcgt ggggcgatcc
     1261 catgccgctc ctttttcagg aggagcgtat cgtatgttta tgaaaggttt tgtttccctt
     1321 ttcgttccgg gcgagtccat tccaacccgt aagctcaccg gtcctgccgg agccgccgcg
     1381 cttgtgctca ccgtcgcgac ggctctcgtc catttctgga tgaacagcat cggcctgctg
     1441 atcgccgtca agatgaacgc cattcacctg ggaacgctca tggcgattat cttcctcttc
     1501 tatccggcct tcgccggttc cccgagagaa cgcccgtctc tccccgactg ggttttggcg
     1561 ctgacctctc tcggatgcat gttttggctg ctcttcacct acgacagact gcttcagacg
     1621 aatcttcagg cgacattccc ggatcttgcc gtcgccgtga ttaccatggc gcttcttgta
     1681 gaagccagcc gccgggcagt aggcttgccg ctgacgatgc tcagtatttt ctttcttgcg
     1741 tacacccgtt tcggacccat gttcccgggg ctctttgcgc accgcggttt caactgggag
     1801 cggatcatta tcagaatggc cctcaccgac caggggatct acggcgttac cctcatggtg
     1861 tcgtcgagtt atgtcttcat gttcatcctc ttcggcgcgt tccttgccgc ctccagaacc
     1921 agcgagttct tcaacgactt ctctcttgcg cttgccggaa aataccgcgg cggacctgcc
     1981 aaggtcgcgg tcatggcgtc cgcgctgatg gggagcatct cggggagcgc gcaggcgaac
     2041 gtggccacaa ccggcgcctt caccattccg ctgatgaaga aggtagggta catgccgcac
     2101 ttcgcaggcg ccgtcgaggc ttgtgcatcc acgggggggc agttcatgcc gccgataatg
     2161 ggcgccggag ccttcataat gagtgaattc ctggggatct catacctcag gatagcggcg
     2221 gccgcgataa taccggcgat gctctactac tccgcccttt tcatcgccgt acacctcagg
     2281 gccaggcaca ggaacc
//
LOCUS       DAOSWW010000409         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37436_length_2294_cov_3.422957, whole genome shotgun sequence.
ACCESSION   DAOSWW010000409 DAOSWW010000000
VERSION     DAOSWW010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37436_length_2294_cov_3.422957"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1163
                     /locus_tag="PK442_08500"
     CDS             192..1163
                     /locus_tag="PK442_08500"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOO87730.1"
                     /translation="MAQMTYMEALRDGMRMEMRRDRNVFLAGEDVGFFGGCFGQTAGL
                     FDEFGKERVVDTPITETAILGLGVGSAAAGLRPIVELMFMDFMGVCMDEINNQAAKMR
                     YMFGGKAKLPMVVRTTCGAGMSAAGQHSQSLEAWLAHIPGVKVVMPATPADAKGLMEG
                     AVRDDNPVFFIEHKGLFGLSGEVPDGEYVVPPGKAAVLREGKDVSLIAWSAMVHKALA
                     AADMLAKDGISAEVIDLRSIAPLDKKTVLDSVCKTGRVVIVHEACRTGGFGGEIAAII
                     ADEAFDSLNAPVKRVTAPDTPVPFNARLEQLYIPSAEKVVATVKELF"
     gene            1203..2231
                     /locus_tag="PK442_08505"
     CDS             1203..2231
                     /locus_tag="PK442_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOO87731.1"
                     /translation="MEKDAFVGIIANPASGKDIRRLVAYGTVFDNQEKVNIVRRILLA
                     LDALGVGRVLAMPDYYGIVRRAAEGVRSDRPLRLGVEMLDMPLTGTQIDSQRAAELLC
                     DEGCGCIVTLGGDGTNRMVAKGCRDVPLMPVSTGTNNVFPLLIEGTTAGLAAGVTALG
                     CGEECTVRRSKKLTIFKNGEAADIALIDAVVLQSRFIGSRAMWEVENMRCCAVTRGEP
                     HNIGVASVVGNLSPITPEDPHGGFLKFDPQRADIFAPIAPGLFLPVGVASFCALQPGE
                     EIQVAPVPSIIALDGEREVDLHAEDSASIRLDRDGPRVVDVPAALRWAAESGFFGNKE
                     LVRRLAPL"
BASE COUNT          403 a          687 c          826 g          378 t
ORIGIN      
        1 gcggcaaaga ttggcggccg cgacgtgaca atcacgcagg ccgatatcga cgcgatgagg
       61 gcgaaggtcg acgaggagac cgaggcggcc attaagttcg ccgaggacag ccccgaaccg
      121 ggcgacgacg aactcctcac cgacgtgtgg gccggttgag cggcgcggat gaccgaagga
      181 gagtggcgaa gatggcacaa atgacatata tggaagcgct cagggacgga atgcggatgg
      241 agatgcgccg ggacaggaac gtctttctcg ccggcgagga tgtgggattc ttcggcgggt
      301 gtttcggcca gacggcgggt ctcttcgacg agttcggcaa agagcgcgtg gtggatactc
      361 ccatcacgga gacggcgatc ctgggcctgg gagtcggttc cgcggcggcg gggctccgtc
      421 ccatcgtcga gctgatgttc atggacttca tgggcgtctg tatggacgag atcaacaacc
      481 aggcggcaaa gatgcgctac atgttcggcg gcaaggccaa gctcccgatg gtggtccgca
      541 ccacgtgcgg cgcggggatg tccgcggcgg ggcagcactc gcagtcgctt gaggcgtggc
      601 tggcacatat tccgggagtc aaggtggtca tgcccgcgac tccggccgac gccaaagggc
      661 tcatggaagg agccgtccgg gacgacaacc cggtcttctt catcgagcac aaggggctct
      721 tcggtctctc gggcgaggta cccgacggcg agtacgtcgt gcctccgggg aaggcggccg
      781 tgctccgcga ggggaaggac gtctcgctga tcgcgtggtc cgcgatggtc cacaaggcgc
      841 tcgccgccgc cgacatgctg gcgaaggacg gtatctccgc cgaggtgatc gacctgcgga
      901 gcatcgcgcc gctggataaa aagactgttc tcgactccgt ctgcaagacg gggcgcgttg
      961 tcatcgtcca cgaggcgtgc cgcaccggcg gcttcggcgg cgagatcgcg gccatcatcg
     1021 ccgacgaggc gttcgacagt ctgaacgccc cggtgaagag ggtgacagcg cccgacacgc
     1081 cggtgccgtt caacgcgcgt ctggagcagc tctacatacc gagcgcggag aaagtcgtcg
     1141 caacggtcaa ggaactcttc taagcgctcc ggagcggccg gggaaagggg aggggctcca
     1201 tcatggaaaa agacgctttt gtcggaatca tcgccaatcc cgcgtcggga aaggacatcc
     1261 ggcgcctggt cgcctacggc acggtcttcg acaatcagga gaaggtgaac atcgtccggc
     1321 ggattctgct ggcgctcgac gctctcggcg tggggcgcgt gctcgcgatg ccggactatt
     1381 acggaatcgt ccgcagggcg gcggaagggg tgcgctccga ccgccccctg cgcctcggcg
     1441 tggagatgct cgatatgccg ctcaccggaa cgcagatcga ctcgcagcgc gctgcggaac
     1501 ttctctgcga tgaggggtgc ggctgcatcg tcacgcttgg aggggacggg acgaaccgga
     1561 tggtggcgaa ggggtgccgc gacgtccctt tgatgcccgt ctccacgggg acgaacaacg
     1621 tctttccctt gctcatcgag gggacgacgg cggggctcgc ggccggggtg acggcgctcg
     1681 gctgcggcga ggagtgcacg gtccggcgga gcaaaaaact cacgattttc aagaacgggg
     1741 aggcggcgga catcgccctc atcgacgcgg tggtgctgca gagccgcttc atcggcagcc
     1801 gagcgatgtg ggaggtggag aacatgcgct gctgcgcggt gacgaggggg gagccgcaca
     1861 acatcggcgt cgcctccgtc gtggggaatc tctccccgat cacgccggag gacccgcacg
     1921 gcggcttcct gaagttcgat ccgcaacgcg cggatatctt cgcccccatc gcgccgggac
     1981 tctttctccc cgtcggggtc gcttcgttct gcgcgctcca gccgggggag gagattcagg
     2041 tcgctcccgt tcccagcatc atcgcgctcg acggcgagcg ggaagtggat ctgcacgccg
     2101 aggattcggc ctccatccgg ctcgacaggg acggcccccg cgtggtggac gtgcccgccg
     2161 cgttgcgttg ggctgcggag agcggcttct tcggcaataa agagctcgtg cggcggcttg
     2221 cgccgctgtg acgatacgta gacacagcgc ccggggtcct gtccttcatg agcggcctcg
     2281 ggctttttat aggc
//
LOCUS       DAOSWW010000410         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37447_length_2294_cov_2.560071, whole genome shotgun sequence.
ACCESSION   DAOSWW010000410 DAOSWW010000000
VERSION     DAOSWW010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37447_length_2294_cov_2.560071"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1364)
                     /locus_tag="PK442_08510"
     CDS             complement(297..1364)
                     /locus_tag="PK442_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOO87732.1"
                     /translation="MNFTPNVAVSRDCFSSLFKMPRARQEKVLEFIRRFSERPDSPGI
                     NFEKIRDAKDKKLRSVRIDDAYRGIVLHPESGNTYVLLWVDHHDDAYAWARNRSCAVN
                     PYTGSLQIFEVCEEKRAAEPAVSETLPLFAGTGDPHQRIYRHPVVLSHCGISVAGRSC
                     KLLVNYRTTEETRRWAVSLLDGRSLDDLEGGEDDLKGYRSLLRGQEPRLEGFGSFDEE
                     ADFLVAFLKEIESKGERLKDVCLVVRSRALLERYREALAGRGLSLVKLEAGGPENRDE
                     DGVRTATMHRVKGLEFDRMILAGVCEDIVPPKSWREHEDPPMMNELETRERSLIHVAA
                     TRARTHILVTWHGEPSPLLGL"
     gene            1541..1801
                     /locus_tag="PK442_08515"
     CDS             1541..1801
                     /locus_tag="PK442_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOO87733.1"
                     /translation="MRICDSSIRVRMDSETKQKASAALEKMGLSVSDAVRLFLVRVGE
                     EERIPFDVEIPNTETRMAMKELETGGGFSCATLEETFKELGI"
     gene            1803..2075
                     /locus_tag="PK442_08520"
     CDS             1803..2075
                     /locus_tag="PK442_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HOO87734.1"
                     /translation="MLSIRYSGRFKRDVRRLENRGKVVEKLKAAMRLLAQQESLPAAY
                     GDHPLKGEWKGYRDIHIEPDWILIYRIAGTELLLVASGTHADIFEI"
     gene            2105..>2294
                     /locus_tag="PK442_08525"
     CDS             2105..>2294
                     /locus_tag="PK442_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87735.1"
                     /translation="MNRQSPQPAEALKRSPQLELGQVPRLFVDPDSGQVQLASAARLS
                     AHRRSGTPIAIRTSSLRRD"
BASE COUNT          467 a          721 c          661 g          445 t
ORIGIN      
        1 atgatggagg atgtcgtgcg ggacgcgggc cccgacaccg accacggcgg cgtggccaag
       61 tacttcgaaa agctcaacgg cgtctcgctg gtgacggaat agggcgtgac gctggcaccg
      121 gcaaaaccgg gaaggcttga gtcaacgcac accgctcgtt ccctttaacc tccatgctga
      181 aacaggtaat atttattacc accttaggca aatatcggta aaattcacgg ccgggtgatg
      241 cgaaatgctg gaaacagcac agtccctgat tctcagcgtc gtcaggctct tccgctttac
      301 agccccagca gcggggaggg ttcgccgtgc caggtcacca ggatgtgcgt ccgcgccctc
      361 gtcgcggcga catggatcag ggagcgctcg cgggtttcca gctcgttcat catcggggga
      421 tcctcatgct cccgccacga cttcggcggg acgatgtcct cgcagacgcc tgcgaggatc
      481 atccggtcga actcaagccc cttcacccgg tgcatcgtcg ccgtccggac gccatcctcg
      541 tcgcggtttt cgggcccccc ggcctccagc ttcaccaagg agaggccgcg cccggcaagc
      601 gcctcgcggt aacgttccag aagcgcccgc gagcggacga ccaggcagac gtccttcaat
      661 cgctccccct tggactcgat ctccttcagg aacgcgacca gaaaatccgc ctcctcgtcg
      721 aacgatccga acccctccag acgtggctcc tgaccgcgca gcagcgaacg gtaccccttc
      781 aggtcgtcct cccctccctc aagatcgtcg agagaacgcc cgtccagcag ggagaccgcc
      841 cagcgcctgg tctcctcggt ggtgcggtag ttcaccagca gcttgcagct ccgcccggcg
      901 acggaaatgc cgcagtgcga gagcacgaca gggtggcggt atatcctctg gtgcgggtcg
      961 cccgtccctg cgaagagcgg cagcgtctcc gagacggccg gttccgctgc tcgcttctcc
     1021 tcgcaaacct cgaagatttg caggctcccc gtgtaggggt tcaccgcgca cgagcggttt
     1081 ctggcccacg cgtaggcgtc atcgtgatgg tcgacccaga ggagcacata ggtgtttccc
     1141 gactccgggt gcagcacgat gccgcggtag gcgtcgtcga tccgcacgga gcgcagcttc
     1201 ttgtccttcg cgtcgcggat cttctcgaag ttgatccccg gagagtccgg ccgctcgctg
     1261 aagcgcctga tgaattccag caccttctcc tgccgggcgc gcggcatctt gaagagcgac
     1321 gagaagcagt ccctggagac tgcgacgttc ggcgtgaagt tcatactgtt cggcctcctt
     1381 cgggggaatg cgttacgcat tcgtttgctt ccgccacaac ggtcctgccg cctggctcca
     1441 aacatccttt ttcaaatatc gcaataccca accggaaaaa gccttgctct cactcccgtt
     1501 cattgctaca atgtgatcac aaagaaagga tgtgtccccg atgagaatat gcgattcgtc
     1561 gatccgcgtc cgcatggata gcgagacaaa acaaaaagct tccgccgcgt tggagaagat
     1621 ggggctttcc gtatccgatg cggtgcgtct ttttctggtg cgcgtcggag aggaagaacg
     1681 gatacctttc gacgttgaaa tccccaacac tgaaacacgc atggcgatga aagagctgga
     1741 gaccggcgga gggttctcct gcgccacgct ggaggaaaca ttcaaggagc tggggatcta
     1801 gcgtgctttc catacgttat tccggacgtt tcaaacgcga tgtgcgccgc ctggagaatc
     1861 gaggtaaagt ggtggaaaaa ctcaaggcgg ccatgcgact cttggcgcag caagagagct
     1921 tgcccgccgc atacggagat caccccttga aaggggaatg gaaaggatat cgggatatcc
     1981 acatcgaacc cgattggatt ctgatatatc gaatcgccgg aaccgagctg cttctggttg
     2041 catccgggac gcatgccgac atcttcgaga tatgaacata cttcagcatc ttgaacgcca
     2101 ataggtgaat cggcaatcgc cgcagccggc ggaagcatta aaacgctctc cccagctcga
     2161 actcggccaa gttccgcgtc tcttcgttga tcctgattcc ggccaggtac agctcgcgtc
     2221 cgcggcccgg ctctccgcac accggcggag cggaacaccc atcgcgatca ggacttcatc
     2281 tcttcggcga gacg
//
LOCUS       DAOSWW010000411         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37465_length_2293_cov_3.406166, whole genome shotgun sequence.
ACCESSION   DAOSWW010000411 DAOSWW010000000
VERSION     DAOSWW010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37465_length_2293_cov_3.406166"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /gene="tsaA"
                     /locus_tag="PK442_08530"
     CDS             complement(<1..331)
                     /gene="tsaA"
                     /locus_tag="PK442_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOO87736.1"
                     /translation="MNEEQMQLFQVGVVRSSLLTRADCPKQGGEGGVEAEIFVAPEYR
                     EGLDGLEPGRDVLLLTWLHRAERDVLKVHPRGNREAPLKGVFATRSPARPNPVGLHRV
                     KILELEKE"
     gene            547..1722
                     /locus_tag="PK442_08535"
     CDS             547..1722
                     /locus_tag="PK442_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO87737.1"
                     /translation="MKAILAGTLETGRGTTQKNATIFLDKGKIVDIAEGLDPKGASEV
                     LDASAFVVTPGLIDAHTHIGTYCEGFPVGMADANDMVHPIAPQLRILDAIYQDDTAFE
                     DALAGGVTCVQTLPGSGNVIGGQGAIIKTSTSVHGRKKVVEEMLVRAPSSMKAALGEN
                     PIRVYTEKGKLPNTRMGNAALMRQALVDAENYRNKQNQAKAKNEAGERNLQHEAMLPV
                     LDGELPLCVHAHRCDDIATAVRIAKEFSIRYTVEHCTEGHLIAPFLAENKVMAAVGPT
                     LTGKPKIELRNKTWDTPVLLWKAGVHFCIITDHPVVPIEHLSVCLSLAVRAGLPREEA
                     LRAVTLSAAEHLGIADRVGSIEKGKDADIVVWDGDPLDARSRAVATIIDGETAYSRI"
     gene            complement(1815..>2293)
                     /locus_tag="PK442_08540"
     CDS             complement(1815..>2293)
                     /locus_tag="PK442_08540"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOO87738.1"
                     /translation="EILPFDNEIHIVVLTGEQLRKAFEIAASSLVGKDDRYESSVRTY
                     AGGFLQISGARVVYSLSGRPTLVDPDGKVLEWGNRVKSLHVERDGAWLPVEDDASYTL
                     AANSWTATGGDRFSVLGDAPQQKTDFKDLDVLADYIASHPDGRVRLELDGRITVEE"
BASE COUNT          440 a          793 c          667 g          393 t
ORIGIN      
        1 tctccttctc aagttcaagg atcttcactc tgtgcagccc taccggattc ggacgcgccg
       61 gagaacgggt cgcgaagacg cccttcagag gcgcttccct gttcccgcgc gggtggacct
      121 tgaggacgtc ccgctccgcc cgatgcagcc acgtcaagag cagcacatcc cgccccggct
      181 ccagaccgtc gagcccctcc ctgtattcag gcgcaacaaa gatctccgcc tccacgccac
      241 cctcgccccc ctgcttcggg cagtccgccc gcgtcagcaa agaagagcgc accacgccta
      301 cctggaacag ctgcatttgc tcctcgttca tcctctctcc ctcttttcct ttatccccgg
      361 gagaatgcac acaccccgcc cggtcttcat tgtattgtat cgcgcactcg aaattggtca
      421 ctattcgtca ttatcttcgt acgcttctta tgcttgcttt gccttttccc agagccgtgt
      481 atgctgtaga ataaagaaag atcaatagta tccgatgctc cggcatcgca aggaggtact
      541 cttttcatga aggcaattct ggccggtacg cttgaaaccg gacgcggcac aacacaaaag
      601 aacgcgacca tcttcctgga caagggaaag atcgtcgaca tcgcggaagg gctcgaccct
      661 aaaggcgctt cggaggtgct cgacgcctcc gcgttcgtcg tcacccccgg gctcatcgac
      721 gcgcacacgc acatcgggac ctactgcgaa gggttccccg tcggcatggc cgacgccaac
      781 gacatggtgc acccgatcgc tccgcagctt cgcattctcg acgcgatcta tcaggacgac
      841 acggcgttcg aggacgccct cgcgggcggc gtcacctgcg tccagacgct ccccggcagc
      901 ggcaacgtca tcggcggaca gggagcgatc atcaagacga gcacctcggt acacggacgg
      961 aagaaggtcg tcgaggagat gctggtgagg gcgccctctt cgatgaaggc ggcgctcggg
     1021 gaaaacccga tccgcgtcta caccgaaaag ggaaagctcc ccaacacccg catgggaaac
     1081 gccgcgctga tgcggcaggc ccttgtcgac gcggagaact accggaacaa gcagaaccag
     1141 gcgaaggcga agaacgaggc cggagagcgc aaccttcagc acgaggcgat gctcccggtg
     1201 ctcgacggcg aactccccct ctgcgtccac gcgcaccggt gcgacgacat cgccacggcg
     1261 gtccgcatcg cgaaggagtt ctccatccgc tacacggtgg agcactgcac cgaagggcat
     1321 ctcatcgccc ccttcctcgc cgaaaacaag gtgatggcgg ccgtaggccc cacgctcacc
     1381 ggcaaaccga agatagagct ccgcaacaag acgtgggaca cccccgtgct gctctggaag
     1441 gcgggagtgc acttctgcat catcacagac catccggtgg tccccatcga gcacctctcc
     1501 gtctgcctct cgctggccgt ccgcgccgga ctgccgcgtg aggaggcgct gcgggccgtc
     1561 acgctctccg ccgccgagca cctgggcatc gcggaccgcg tcggctcgat cgagaagggg
     1621 aaggacgccg atatcgtcgt ctgggacgga gaccccctgg acgcgcgaag cagggccgtc
     1681 gcgacgatca tcgacggcga gactgcctac tccagaatct gacgccggaa tccgacgaac
     1741 ggggggcggc cgcgcctgcg cagaccgccc cccgttatca ttctctcccg gcctctccag
     1801 aacgctcccc gccgctactc ctccaccgtg atccgtccgt ccagttcgag gcgcacccgc
     1861 ccatccgggt gactggctat gtagtcggcg aggacgtcga gatccttgaa gtccgttttc
     1921 tgctgcggcg cgtcgccgag caccgagaat ctgtcgcctc cggtcgccgt ccacgaattc
     1981 gcggcaagcg tgtacgaggc gtcgtcctcg acgggcagcc atgccccgtc gcgctccaca
     2041 tggaggctct tgacgcggtt tccccactcg agtaccttgc cgtcgggatc gaccagcgtc
     2101 ggacggccgg agagcgagta gacgacgcgc gccccggaga tctggaggaa ccctcccgcg
     2161 tacgtgcgaa cggaggactc gtatctgtcg tcctttccca cgagggagga cgcggcgatt
     2221 tcaaaggcct tccggagctg ttcgcccgtc aggacgacaa tgtggatctc gttgtcgaag
     2281 gggagaatct ccg
//
LOCUS       DAOSWW010000412         2291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37530_length_2291_cov_2.388193, whole genome shotgun sequence.
ACCESSION   DAOSWW010000412 DAOSWW010000000
VERSION     DAOSWW010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37530_length_2291_cov_2.388193"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..94
                     /locus_tag="PK442_08545"
                     /pseudo
     CDS             2..94
                     /locus_tag="PK442_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            complement(225..1475)
                     /locus_tag="PK442_08550"
     CDS             complement(225..1475)
                     /locus_tag="PK442_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO87739.1"
                     /translation="MKKIIVLLLLLAGGAGYFFLSDGGATEYTFRTAALESGNIVAKV
                     TATGKLSAVTVVEVGTQVSGTVKEIYADFNDHVKKGQLIALIDPDVLSAKLEESKANL
                     TVARASVARAKANLADSQRNLKRNKELWSRNLIAKSELEAAETTHALNRASLQEAEAR
                     VLQVQAALRQSESNLGYTRILSPEDGVIISRLVNVGQTVAASLSTPTLFSIAKDLTDM
                     QIETSVDEADIARVKEGQEVEFTVDAYRGTVFPGTVRQVRIAPGTTDNVVTYPVIISV
                     ANPELKLKPGMTANVSIITDRRTGVLRVPMAALRFTPPPTEAPVAVATATSSPFAPSM
                     PRRPRGGGAGNGQGGGAGTNGAFSSIWTVRDGKLAEQINFRAGIGDGSFIEVAGSKEL
                     KEGDLLAIAYSEKPKVSFWKRIFQ"
     gene            complement(1472..>2291)
                     /locus_tag="PK442_08555"
     CDS             complement(1472..>2291)
                     /locus_tag="PK442_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO87740.1"
                     /translation="RREQAESAVRTYEKHLEKAKGFYEAGIKARFDVTKAEVDLSNAR
                     ITLVSAEASLATARAALSNAVGIQLGDVEAVSGFLAPRSLPDEPFAVEQAMANRPDIR
                     SALLKSESGKLSVAIAAKGNAATVSLSGSANLSGSEWPLNDTFRATVTLSVPVFDGGL
                     TDARIAEARYGAQGADAALRRVEQTVLYDVRSTLLSVKEAEARIPVAELLVRQAEENL
                     TLAEGRYEMGVGNVLEVADAVLAFTSAKVSLYQAQHDYSASLAALEKTLGGEFE"
BASE COUNT          327 a          783 c          761 g          420 t
ORIGIN      
        1 gatcaagttc ttcgacgcgg acaaggggtt cggcttcgtg tcggtcgacg acggcgacgg
       61 cgggggcgac gtcttcgtcc ccgcgagcgc gctgccggcc ggcgtgacga cgcttcgcgg
      121 cggcgcccga gcgcgccgcc ttccgcgcct gacgccgtcg agcgcccgca gctccacgtc
      181 gccggtatgg tagatcttga caatgtcgcg gacgtcgatc aggctcattg gaagatcctc
      241 ttccagaacg aaaccttcgg cttctcggag tatgcgatgg cgaggagatc cccctccttc
      301 agctctttcg acccggccac ctcgatgaag gagccgtcgc cgatccccgc gcggaagttg
      361 atctgctctg cgagcttccc gtcgcgcacg gtccatatgg aggagaacgc cccgtttgtt
      421 cccgcgccgc ctccctgccc gttgccggct ccgccgcccc tggggcgccg cggcatggag
      481 ggggcgaagg gcgagcttgt ggctgttgca actgctacgg gagcctccgt cggcggaggg
      541 gtgaagcgca gcgcggccat cggcacgcgg agcacgccgg ttctgcggtc ggtgatgatg
      601 gagacgttgg ccgtcatccc cggcttcagc ttcagctcgg gattggccac cgagatgatg
      661 accgggtagg tgaccacgtt gtccgtcgtc cccggcgcga tgcgcacctg cctcaccgtg
      721 ccggggaaga cggtccccct gtacgcgtcc acggtgaact ccacctcctg cccctccttc
      781 acgcgggcga tgtccgcctc gtcgacggag gtctctatct gcatgtccgt caggtccttg
      841 gcgatggaga agagcgtcgg cgtggagagg ctcgccgcga cggtctggcc gacgttgacc
      901 aggcgcgaga tgatcacgcc gtcctccggc gagaggatgc gcgtgtaccc caggttggac
      961 tccgactggc gcagagcagc ctgtacctgg agcacgcgcg cctccgcctc ctgaaggctc
     1021 gccctgttga gggcgtgcgt ggtctccgcc gcctccagtt cgcttttcgc gatcaggttg
     1081 cgcgaccaca gctccttgtt ccgcttcaga ttcctctggc tgtcggcgag gttggccttc
     1141 gcccgagcga ccgacgccct ggcgaccgtg aggttcgcct tcgactcctc cagcttcgcg
     1201 ctgaggacgt ccgggtcgat gagcgcgatg agctgtccct tctttacgtg gtcgttgaag
     1261 tcggcgtaaa tctccttgac ggtcccggag acctgcgtgc cgacctcgac cacggtgacc
     1321 gcgctcaact ttccggtggc cgttaccttc gcgacgatgt tccccgactc cagcgcggcg
     1381 gtgcggaacg tgtactccgt agcgcccccg tccgagagga agaagtaacc ggcgcccccg
     1441 gccagcagaa gcagcagtac gataattttt ttcattcgaa ctcacccccg agggtcttct
     1501 ccagcgcggc cagcgaggcg ctgtagtcgt gctgtgcctg atagaggctt accttcgcgc
     1561 tggtgaaggc gagcacggcg tccgccactt cgaggacgtt ccccacgccc atctcgtagc
     1621 gtccctccgc gagcgtcagg ttctcctccg cctggcgcac cagcagttcc gccaccggga
     1681 tgcgcgcttc ggcctctttc acggagagga gcgtcgagcg gacgtcgtag aggacggtct
     1741 gttcgacgcg ccgcagcgca gcgtccgccc cctgcgcgcc gtagcgggcc tccgcgatcc
     1801 tggcgtccgt cagcccgccg tcgaagacgg ggacggagag cgtgaccgtg gcgcggaagg
     1861 tgtcgttgag cggccactcc gaaccggaga ggttcgcgga gcccgaaagg gagaccgtcg
     1921 cggcgttccc cttcgcggcg atggcgaccg agagcttgcc cgactcgctc ttcagaagag
     1981 cggagcggat gtccggcctg ttcgccatgg cctgctcgac cgcaaagggc tcgtcgggga
     2041 gcgaacgggg cgcgagaaag ccggagacgg cttcgacgtc gccgagctgg atgccgaccg
     2101 cgttcgacag agctgcacgc gccgtggcga gcgacgcctc cgcggagacg agggttatcc
     2161 gggcgttgct caggtccacc tcggccttgg tgacgtcgaa ccgcgccttt atcccggcct
     2221 cgtagaagcc cttcgccttc tccagatgct tttcgtaggt gcgcacggcg ctctccgcct
     2281 gctcgcgcct c
//
LOCUS       DAOSWW010000413         2290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37548_length_2290_cov_3.173602, whole genome shotgun sequence.
ACCESSION   DAOSWW010000413 DAOSWW010000000
VERSION     DAOSWW010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37548_length_2290_cov_3.173602"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1065)
                     /locus_tag="PK442_08560"
     CDS             complement(202..1065)
                     /locus_tag="PK442_08560"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87741.1"
                     /translation="MKRVVAAFPGTCGELFQGTLDGVQCLVSCPVDRMARLELLLEEG
                     EGISVPPEMSKTRRALELALARRPLAGALRVRRLEALPEGKGYASSTADILAALYGFA
                     QLSGTPLPPEEASRIALAVEPTDSIAWPGLALLDHREGRVMRFLGPPPPLAVLVVDEG
                     GVVDTEEFNRRDSRALLASLAAAHKEAFALLKKGVERGDPAEVGEAATLSAEACRRIL
                     EKPLLERCRALVRSLGGYGVCVAHSGTLYGLLLPVALLNEKEHVTEAAARALPEAAEI
                     RLHALAAGGPR"
     gene            complement(1062..>2290)
                     /locus_tag="PK442_08565"
     CDS             complement(1062..>2290)
                     /locus_tag="PK442_08565"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOO87742.1"
                     /translation="MGKPWKTLSAGEYYACREVLWSSVVASFEKLASENDLVLVEGAG
                     SPAEINLKKHEIVNMRVARTFGCPVLLAGDIDRGGVFASLVGTLALLDEEERALVKGF
                     LINKFRGDVKLLEPGLDMLSGLTEGRPVLGVVPWLKNLAVAQEDSVWLEERTAGDSSG
                     GVDIAVIKLPRISNYDDFDPLALEEGVSLRFVERPEDLGHPAAVILPGSKTTLKDLAW
                     LRSSGFDGLIAALALAGGSVVGVCGGYQMLGRRISDPLGVEGPASEESGLGLLPVETV
                     FEPAKETTLSRGRTIPSSESPASGFLAQPFDVEGYEIHMGRSVLPDGARPLLRFEDGR
                     VDGAVSAGGHVWGTYLHGIFDNGPFRTAWLRSLGWKGKESPLSFAQVREQAFDRLADE
                     VEGALNMELLERIIGL"
BASE COUNT          423 a          837 c          693 g          337 t
ORIGIN      
        1 ccacgagcac gccgccgaag atgagcatct tccggtcgtc cgcaccagac ggacggcggt
       61 tccagaacgc accgatgtac tttcccatcc agaccacagg atgcaccttc gacgagggct
      121 ccccaagcag gagatcccac actgcggcgg cgaagagaac gacaaacact tccatgcgaa
      181 taccttcctt tcgaacggat tctaccgcgg tcccccggcc gccagggcgt gaagacgaat
      241 ctccgccgcc tccggcagcg cgcgcgcggc ggcctccgtt acatgctctt tttcattcag
      301 cagggcgacg gggaggagca gaccgtagag cgtgccgctg tgcgcaacgc agacgccgta
      361 ccccccgagc gaacgaacga gcgcgcgaca ccgctccaac agcggttttt ccagaatgcg
      421 gcggcacgcc tccgcgctga gcgttgcagc ctcccccact tcggcggggt ctccccgctc
      481 cacgcccttc ttcaacagag cgaacgcctc tttatgcgca gcggcgagcg aagcgagaag
      541 agcgcggctg tccctccggt tgaactcctc ggtgtccacg acgcccccct cgtccaccac
      601 gagcacggcg agaggcggag gcgggccgag aaaccgcata acgcgcccct cccgatggtc
      661 gagcagcgcc aggccaggcc atgcgatgct gtccgtcggt tcgacggcaa gcgcgattcg
      721 cgacgcctcc tccggcggca gcggcgttcc tgaaagctgc gcgaaaccgt agagcgcggc
      781 aagaatatcc gccgtgctgc tggcataccc ctttccctcc ggaagcgcct caaggcggcg
      841 aacccgcagc gcgccagcga gagggcgacg ggcaagcgcc agttcgagcg ctctccgcgt
      901 cttgctcatc tcgggcggaa ccgaaatgcc ttccccctct tcaagcagca gttccagccg
      961 ggccatcctg tcgacggggc aggagacaag gcactgcacg ccgtcgagcg tcccctgaaa
     1021 aagctccccg cacgttccgg ggaaggccgc gacgacgcgc ttcatagccc gatgatccgc
     1081 tccagcagct ccatgttgag cgcgccctcc acctcgtccg caaggcggtc gaaggcctgc
     1141 tcacggactt gggcgaagga gagcggactt tcttttccct tccagccgag cgagcgcagc
     1201 catgcggtcc ggaacgggcc gttgtcgaag atgccgtgca gataggttcc ccagacgtgt
     1261 ccgcctgcgg agaccgcgcc gtcgacgcgg ccgtcctcga accggagcag cgggcgagcc
     1321 ccgtcaggca gcacgctccg tcccatgtgg atctcgtacc cctccacgtc gaacggctgc
     1381 gcaagaaaac cggaggcggg gctctccgaa gacggtatcg ttcgccctct tgaaagcgtc
     1441 gtctccttcg ccggttcgaa gaccgtctcg acgggcagca gaccgagccc ggactcctcc
     1501 gacgcggggc cctccacgcc gagcgggtcg gaaatgcgcc gtccgagcat ctggtagccg
     1561 ccgcagacgc cgacgacgga acctccggcg agcgcgaggg cggcgatcag tccgtcgaag
     1621 ccggaactgc gcagccaggc caggtccttc agcgtcgtct tgctccccgg aaggataacg
     1681 gcagccggat ggcccaagtc ctccggacgc tccacgaagc ggagcgagac cccctcttca
     1741 agcgcgagcg ggtcgaagtc gtcgtagttg gagatgcggg ggagcttgat cacggcgatg
     1801 tccacgccgc cggaggaatc gcccgccgtg cgctcttcaa gccacacgga gtcctcctgc
     1861 gccacggcga ggttcttcag ccacggaacg acgccgagca ccggacgccc ctccgtgaga
     1921 ccggaaagca tgtcgagtcc cggttccagc agcttcacat ccccccggaa cttgttgatc
     1981 aggaacccct tcaccagcgc gcgctcctcc tcgtcgagca gcgcgagcgt cccgacaagc
     2041 gaggcgaaaa ccccgccccg gtcgatatcc cccgcgagaa ggaccgggca gccgaacgtg
     2101 cgcgccacgc gcatgttcac gatctcgtgc ttcttcaggt tgatctccgc cgggctcccc
     2161 gccccttcga cgaggacgag gtcgttctcc gaggccagct tctcgaacga cgcgacgacc
     2221 gaactccaca aaacctcccg gcacgcgtag tactcccccg ccgaaagcgt cttccacggc
     2281 ttccccatca
//
LOCUS       DAOSWW010000414         2282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37744_length_2282_cov_3.973058, whole genome shotgun sequence.
ACCESSION   DAOSWW010000414 DAOSWW010000000
VERSION     DAOSWW010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37744_length_2282_cov_3.973058"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PK442_08570"
     CDS             <1..324
                     /locus_tag="PK442_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87743.1"
                     /translation="RLLDSPKGKYFFISAAPAEYSDIVRMLTERGVVPSFGKWPEAAV
                     EGTLPLGAPVRGRVSRDRAISEGMTFLNGAGQPAPNGMYAWDRKKGRIYDVVDVERRR
                     FWFYD"
     gene            complement(426..1202)
                     /locus_tag="PK442_08575"
     CDS             complement(426..1202)
                     /locus_tag="PK442_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOO87744.1"
                     /translation="MPSFFSVLFRASAHHPLNRRLFLLAVLLFFFQSAACANELPWVE
                     HSVLRGETADSIAAKYAVSSKTVERANELAARTEPLKTGEKLLVPKKESDLLSTLAEV
                     RARRRGESPLSSITREQSKKVSLPAPRKSPLPRPAEPFIRPLAGRITSPYGMRGGRLH
                     DGVDIPAPKGTPIVASRPGKVVFSGTIQGFGRTVTIDHGDGMKTRYSHNSANLVKKGD
                     VVRQGQPIAKVGRTGRATCTHVHFSVLINGKPINPEKYLR"
     gene            1475..>2282
                     /locus_tag="PK442_08580"
     CDS             1475..>2282
                     /locus_tag="PK442_08580"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOO87745.1"
                     /translation="MGTLSITQLKSGMILSADLVAPNGRFILPKGACLTSRNIMSLKI
                     WGVSEVAVSDRSDHPCEEDEFLYSSSVMEEARTLVLRIFGGIPPGNRAMEEVYRVAIR
                     EAARSLTENEARPPVFFRPEFTPPSGAPLPKLAMEELVGAEVRFVSFPDVYFRISEAL
                     QSPKSSITHVAELISKDTALSATLLKLANSAIYGVPAKVDSIQRAAALIGGKALSILA
                     LGVSAVKSFNSIPSEWVNMESFWRHSVAVAVLGQIISSRKNSRIIEKLFVG"
BASE COUNT          441 a          648 c          697 g          496 t
ORIGIN      
        1 cgcctgctgg actccccgaa agggaagtac ttcttcattt ccgccgctcc ggcggagtat
       61 agcgacatcg tccggatgct gacggagcga ggcgtcgttc cctccttcgg aaagtggccg
      121 gaggccgccg tggaggggac gctgccgttg ggggccccgg ttcgcgggcg cgtatcccgc
      181 gacagggcga tctcggaggg aatgaccttt ttaaacggcg ccggacagcc tgcgccgaac
      241 ggaatgtatg cgtgggacag gaagaagggg agaatttacg acgtcgtcga cgtggagcgg
      301 cgccgcttct ggttctacga ctgatcctta tgcagctttc ttgtagttcc atttataagg
      361 aagaaggcgc gttccctgtt ttcgggacgc gccttctttt ctttgaagat aaaggaagtt
      421 tccgctcagc gaaggtactt ctccgggttg atcggcttac catttataag gacgctgaag
      481 tgaacgtgcg tgcaggtcgc ccttccggtt ctgcccacct tcgcgatagg ctgcccctga
      541 cggacgacgt cccctttttt gacgaggttg gcgctgttgt ggctgtaccg ggtcttcatc
      601 ccgtcgccgt ggtcgatggt caccgtccgg ccgaacccct ggattgttcc ggagaagacg
      661 accttgccgg ggcgcgacgc gacgatcggc gttcccttgg gagccgggat atccacgccg
      721 tcgtgcaggc gtccgccccg cattccgtag ggagaggtga ttcttccggc gagcggacgg
      781 atgaagggct cggccggtcg cggcaggggg ctcttcctcg gcgcgggaag agaaaccttt
      841 ttcgactgct ccctcgtgat cgaactgagc ggtgattcgc cccgccgccg ggcgcggacc
      901 tcggcgaggg tggagaggag atcggattct ttcttcggaa cgaggagctt ttcccccgtc
      961 ttcagaggtt ccgtccgggc ggcaagttca ttggcccttt cgacggtctt tgaggaaacg
     1021 gcgtacttcg ccgcgatgga gtcggcggtc tctccgcgga ggaccgagtg ttcgacccac
     1081 ggaagttcgt tcgcgcacgc ggcggactgg aaaaagaaaa gaagaacagc gagaagaaaa
     1141 agacgacggt tcaacggatg atgcgcagaa gccctgaaca ggacgctgaa gaaagagggc
     1201 atgcggagtt cctcctgaac tttattttat gacgctctat ggtatgatac atgaaaacag
     1261 aaggttgcgg acggtaaaaa tactgagaac gaatttacta caagggcgtc tttctgttcc
     1321 gtttttgaac attaaggggc gacattttcg ctctcccgtg acagcttcgt gtccaagccc
     1381 ttgacagcgt ctgccctctg tggttaaaaa ggagagcggt gtaaattttt tttattctcc
     1441 cctttgaacc gtgaggggaa ttccgggggg ttgcatggga acactttcca tcactcaact
     1501 gaaatccgga atgattcttt cggcggactt ggtcgccccg aacgggcggt tcattctgcc
     1561 gaagggcgca tgcctgacgt ccaggaacat catgagtctg aagatctggg gagtatccga
     1621 ggttgccgtg tcggaccgtt cggatcatcc ctgcgaggag gacgaatttc tgtactcctc
     1681 ctccgtcatg gaggaagcgc gcacgctcgt cctccgcata ttcggcggca tcccccccgg
     1741 aaaccgggcg atggaagaag tctatcgggt cgccatccgc gaggccgcgc gaagcctgac
     1801 ggagaatgaa gcgcgtcccc ccgttttttt caggccggaa tttaccccgc cttccggagc
     1861 gccgctgccg aagctcgcga tggaagagct tgtcggggcc gaagttcgtt tcgtctcctt
     1921 ccccgacgtc tacttccgca tatccgaggc gcttcaatcc cccaagagct ccatcacgca
     1981 cgtggccgaa ctcatcagca aggatacggc cctctccgcc acgcttctga aactcgccaa
     2041 cagcgccatc tacggcgttc cggcgaaggt ggactccatt cagcgcgccg ccgccctcat
     2101 cgggggcaag gcgctctcca tcctcgccct cggagtatcg gcggtgaaat ccttcaacag
     2161 catcccatcc gaatgggtga acatggaatc cttctggaga cactcggtcg ccgtggcggt
     2221 cctgggacag atcatctcgt cgaggaagaa ttcgcggatc atagaaaaac ttttcgtcgg
     2281 gg
//
LOCUS       DAOSWW010000415         2280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37802_length_2280_cov_3.597303, whole genome shotgun sequence.
ACCESSION   DAOSWW010000415 DAOSWW010000000
VERSION     DAOSWW010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37802_length_2280_cov_3.597303"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /gene="nhaC"
                     /locus_tag="PK442_08585"
     CDS             complement(<1..1501)
                     /gene="nhaC"
                     /locus_tag="PK442_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOO87746.1"
                     /translation="MEQTSAKTPTFGQALFVLLVDAAIISYGVLDIHFESGAVFGLAL
                     SAHIPLFLAAVFTAIMGAIFVGKPWREVEEGMINGITVALQAVLILLAVGGLVGAWIL
                     SGVVPTLIFYGLNLLSPQYFLLASLLICAIVSLSTGSSWSTSGTVGIALMGIGAGLGV
                     PAPVTAGFVISGAYFGDKISPLSDTTNLAPAVAGSELFTHIRAMLWTTGPTLIIVAGI
                     AYYMGSGYTGGEMDASRISIIQQLMSAEFSISMYGFLPPLLVLVLAAMKIPAIPGIVA
                     GMLLASVMALFQGISIPDVMSALYSGYSPSMIAEVAEAGDISSVNELLKGAAVFSADA
                     FESVKEVAGMLNGLLERGGLTGMLGTVALILVALVLGGIMETCGFLEVLLVRLMQSVK
                     SVFGLVASVLVSCVFTNMFLGDQYIALVMPGRMFKPAFEKFEQNGKKLDPSLLSRSLE
                     DSGTLTSVLVPWNTCGAYNSGVLGVPTLSYLPYAFLNYLNPLVALLMTRL"
     gene            complement(1682..>2280)
                     /locus_tag="PK442_08590"
     CDS             complement(1682..>2280)
                     /locus_tag="PK442_08590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO87747.1"
                     /translation="AIIRGLHLDPGVSARIRWSRINTLAEAYVGSMASSLSFEEKTLR
                     AHFEKNKQKYRQNESVRVRHVFTTEEENARTALLRILSGEVFAEVAASLSVDYATAET
                     GGDMGWISRGSLPESFEEAVFSAPLQSVLGPVQTKYGYHVFEVLERRRARALSFEEVR
                     DAVRAELVEHVMATEAASLKARFPVLLVPSALEKSFAR"
BASE COUNT          642 a          594 c          591 g          453 t
ORIGIN      
        1 cgagccttgt catcaacaga gcaacaagag gattcaggta attcaggaat gcgtacggaa
       61 gatatgacag agtgggcacg ccaaggacac cgctattata ggcgccgcac gtattccacg
      121 gaacaagaac cgaggtgagc gttccggaat cctcaagcga tctggagagc aggctcggat
      181 caagtttttt cccgttctgt tcgaattttt caaaggcggg tttgaacatt cgtccgggca
      241 tgacaagcgc aatgtattgg tcgccaagga acatgttagt gaagacgcac gatacaagaa
      301 cagaagcgac aagaccgaac accgatttga cgctctgcat caagcgaacc aaaaggactt
      361 caaggaaacc gcacgtctcc ataataccgc caagaaccaa agcgacgagg atgagcgcca
      421 ccgtcccaag catgccagtt aaacctcctc tttcgagaag accgttcagc atgcctgcaa
      481 cctccttgac agattcgaac gcatccgcgg agaagaccgc cgctccttta agaagctcgt
      541 tcactgaaga gatatccccg gcttcggcca cctcggctat catggaggga gagtatcccg
      601 aatacagagc gctcataaca tccggaatac tgattccctg gaaaagcgcc ataacggaag
      661 ccaagagcat accggcgaca atacccggga tagcggggat cttcatcgcc gcaaggacga
      721 ggacaagaag cggaggaagg aaaccgtaca tcgagatgga gaactctgca gacatcaact
      781 gctgaataat cgagatcctg gaggcgtcca tctcgccccc agtgtacccg cttcccatat
      841 aataggcgat tccggcaacg atgatcaggg tgggaccggt agtccagagc atcgccctga
      901 tatgggtaaa aagttccgaa ccggctacag caggagcaag gttcgttgta tcggagagag
      961 gagaaatttt atcgccgaaa tacgcgcccg agattacaaa gcccgccgtt acaggagcgg
     1021 gtactccaag ccctgcgcct attcccatga gcgctattcc cacggttccg ctcgtgctcc
     1081 aggaacttcc cgtggaaaga gagacaatgg cgcagatcag gagagatgcc aggaggaagt
     1141 actgaggaga cagaaggttg agaccgtaaa aaatgagcgt gggcacaacg ccgctcagaa
     1201 tccatgcccc aacaagacct cccactgcaa gcaatataag gacggcctga agcgcgacag
     1261 taataccgtt gatcattcct tcctcaactt cacgccacgg tttaccgaca aaaatagctc
     1321 ccataatggc ggtaaagact gcggcgagga agagcggaat atgcgcggag agggcgagac
     1381 cgaacaccgc ccccgattca aaatgaatat cgagaacccc ataactgata atagcagcgt
     1441 ccacaagaag tacaaaaaga gcttgaccaa aagtaggtgt ctttgcagat gtttgttcca
     1501 tacgcaatcc atcctttccg aaagagcggg ataacgcctt tgcaagaaga cgcttgtcct
     1561 tttattataa gagattattt tcttctgtcc atgagtttcg gacggagaag agaataaaaa
     1621 aaggtgcgga caggctccgc accttaggta tttcatcatg ttatgtgtta gagagaaggt
     1681 atcaccgcgc aaatgatttt tcgagagcgg aaggtacaag aagaaccggg aagcgcgcct
     1741 tcagggaagc cgcttcggta gccataacgt gttcaaccag ttcggcgcgc acggcatcgc
     1801 gaacctcttc gaacgagagc gcccgagcgc gacgcctttc aagtacttcg aaaacatgat
     1861 acccgtattt cgtttgcacc ggaccgagta cagattgcaa gggcgctgag aatacggctt
     1921 cctcgaagct ttcggggagt gagccgcgtg aaatccaccc catatcgcct cctgtttcgg
     1981 ccgtggcgta atcaacgctg agcgatgcag cgacttcggc aaaaacttct cccgagagaa
     2041 tgcggagaag cgctgttcgc gcattctcct cttctgtggt gaatacatgc ctgacccgga
     2101 ctgattcatt ctgtcggtat ttctgtttat tcttttcaaa atgagctctc agggttttct
     2161 cttcaaaaga gagcgaagag gccatactgc cgacatacgc ctcggcaagc gtattgatgc
     2221 ggctccaacg gatacgggcc gaaactccag gatcaagatg caatcctcgg ataattgccc
//
LOCUS       DAOSWW010000416         2278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37858_length_2278_cov_3.796221, whole genome shotgun sequence.
ACCESSION   DAOSWW010000416 DAOSWW010000000
VERSION     DAOSWW010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37858_length_2278_cov_3.796221"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2016
                     /locus_tag="PK442_08595"
     CDS             <1..2016
                     /locus_tag="PK442_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOO87748.1"
                     /translation="GQYPAFEPPFRRFRDFSGEIQALGVLDDNGVLYDTATPELASIR
                     QDVDSLRRRIRRAAQSLLDTPSVSQMLQDRVLAYRNSHFVFLVRQEYVNRFPGTVVDR
                     SGSGNSIYMEPSVLVPLNNTLALRLRDQEEEEHRILRKLTSDILARERPILDAQDVLA
                     EFDLLYGGAEVLSKRRWKLPRIEEKTSFSLHQARHPLLGEKAVPVNIKCGEGFRSLVI
                     TGPNTGGKTVVLKTAGVCIYLAWCGLPIPAEEDSRVGNIGAIFADIGDEQSIEQNLST
                     FSAHVKNIISILEQADRTSLVLLDELGAGTDPQEGAALGVALLDTLTKEKGLTLATTH
                     HNPVKQYALTAPGVETASMEFDVETLSPTYRMLLGVPGKSNALFIAQRYGMPSAVLDK
                     ARKVLSEREVPVEELIGELNERRAWLDKEENEIRALRRTLAEERKKYQDSSRDIESRK
                     DRILAEAERKAESILERAEESSKELLRNVHDAAKSAVHRKISETSERVRNERKTLEQR
                     QERRALRGTAMQEGFAPVVGAVAQIAGTDYVGVIEKLQGEKAILRIGAIKMDVNAKKL
                     VKTEKKPKGASLPRDTSMEETPAAVPSSIMVRGMNVQDALPMVERYLDQALRRGHTSV
                     TVIHGRGEGILRREIHALCASLKYVESYRLGDAGEGGYGVTIVTFRR"
BASE COUNT          509 a          651 c          701 g          417 t
ORIGIN      
        1 gggcagtacc ctgcgtttga accccctttc cgccgcttca gggatttctc gggagagata
       61 caggcgctcg gcgtcctcga cgacaacggt gtactgtatg ataccgcgac ccctgagctg
      121 gcctctatac gccaggatgt cgattccctg cgcagaagga tccgcagagc cgcccagtcg
      181 cttctcgaca ccccctcggt ctcgcagatg ctccaggaca gggtgctcgc ctacagaaac
      241 agtcactttg tcttcctcgt tcgtcaggag tacgtgaacc ggtttccggg aaccgttgtc
      301 gacaggtcgg gctccgggaa ctccatctat atggaaccgt cggtgctcgt gccgctcaac
      361 aatacgctcg ccctcaggtt gcgcgaccag gaagaggaag aacatcgtat tctccggaag
      421 cttacctcgg acatactggc gcgggaacgt ccgattctcg acgcgcagga tgtgcttgcc
      481 gagttcgacc tcctctacgg cggcgccgaa gtgctctcca aacgccgctg gaagctcccc
      541 cggatagaag agaagacatc tttttcactc caccaggcgc ggcatcccct cctcggggaa
      601 aaggccgtcc ccgtgaacat caagtgcggc gaaggatttc gaagccttgt gatcacaggc
      661 ccgaatacgg gcggaaagac ggtcgtcctt aaaaccgcgg gcgtctgcat ttacctcgcg
      721 tggtgcggtc ttccgattcc ggcggaggag gactccaggg tggggaacat cggcgccatt
      781 ttcgcggaca tcggcgacga gcagagcatc gagcagaacc tgtccacctt cagcgcccac
      841 gtcaagaaca ttatctcgat tctggaacag gccgaccgga cgtcgctcgt ccttctcgac
      901 gaactcggcg cgggcaccga ccctcaggag ggcgcggccc tcggagtggc gctgctggat
      961 acgctgacga aggagaaagg gctcaccctg gcgacgacgc atcacaaccc tgtaaaacag
     1021 tacgcgctga ccgctcccgg cgtcgagacg gcgagcatgg aattcgacgt ggagacactt
     1081 tcgcccacct acagaatgct gctcggcgtt ccgggcaaga gcaacgcgct ttttatcgcg
     1141 cagcggtacg gtatgccttc cgccgtcctc gacaaggcgc ggaaggtact gtccgagcga
     1201 gaagtcccgg tggaagagct catcggcgaa ctgaacgaac ggagggcgtg gctcgacaag
     1261 gaggagaacg agatacgagc gctccgaaga acgcttgcgg aagagcggaa aaaataccag
     1321 gactcctccc gcgacatcga atcaaggaaa gaccgtatcc tcgccgaagc ggagcggaag
     1381 gcggaaagta tcctcgaacg ggccgaggag tcgagcaagg agctcctccg gaatgtgcac
     1441 gacgcggcga agtcggcagt gcacagaaaa atttcggaga catcggaacg cgtcaggaac
     1501 gaacgaaaaa cactggaaca gcgccaggaa cgaagagcgc tgagaggaac cgcgatgcag
     1561 gaggggtttg cgcctgtagt aggggctgtc gcgcaaattg cgggaacgga ctatgtcggc
     1621 gttatcgaaa aactccaggg ggagaaggcc attctccgca tcggggcgat caagatggac
     1681 gtcaacgcga aaaagcttgt caaaacggag aagaagccca aaggggcgtc cttgcctcgt
     1741 gatacttcga tggaggagac cccggcggcg gttccttcgt ccatcatggt cagagggatg
     1801 aacgtgcagg atgctttgcc catggtcgag cggtaccttg accaggcgct tcgtcggggt
     1861 cataccagcg taaccgtgat ccacgggaga ggggaaggga ttctgcgccg cgagattcac
     1921 gcgctgtgcg cctccctgaa gtacgtggaa tcctaccgtc tcggcgatgc cggcgaggga
     1981 gggtacggcg tcactattgt gacgttcaga cgctgatgca gcatacaaca gcatcgactc
     2041 gtttgtagcg atcctccccg aaatgtcaac ctgcgacatc gtatgaagga tacgttttga
     2101 gcatcggctg gaggatttaa tgaaggttcc aaagagagtt gcacttttgc acacacaatt
     2161 ttctcagcct tgacgccgtc ctctttcgag ggtagaatag gcttcgcctt ttggggctcc
     2221 gcttgcgcct gtagctcagc ggatagagta tcggcctccg gagccgaggg tctggcgt
//
LOCUS       DAOSWW010000417         2277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_37891_length_2277_cov_3.076958, whole genome shotgun sequence.
ACCESSION   DAOSWW010000417 DAOSWW010000000
VERSION     DAOSWW010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37891_length_2277_cov_3.076958"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PK442_08600"
     CDS             complement(<1..140)
                     /locus_tag="PK442_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOO87749.1"
                     /translation="MMYDVIIVGSGPAGIFAAMECVRQEKKVLIIDKGRLIRERKCPI
                     VEG"
     gene            complement(234..596)
                     /locus_tag="PK442_08605"
     CDS             complement(234..596)
                     /locus_tag="PK442_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87750.1"
                     /translation="MALRVCEYCGKAWFDEGGEKQVALCPPCAQEVQGIYMLAWGYLR
                     DNTAEAGRHRKISIQQLADELGIDTKAINLLVKMGKIQVESFAGERNADELARNLEIL
                     RKLRKNVRGKSKYDRSGR"
     gene            complement(601..1254)
                     /locus_tag="PK442_08610"
     CDS             complement(601..1254)
                     /locus_tag="PK442_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOO87751.1"
                     /translation="MGRVQIIVEGMTASGKSTVVNLLSERLGLRVMPEEFRDQYDLLR
                     RFHLERRWAFPMQLNFLVTRFAQYLCASESDEYILDRSVFGDKIYASLYYKLGYFRDS
                     QFGTYLMLYDSLLKHLAMPKLIIVLSCPFEEIMRRIRMRGRSDEIEAGEEYWRSLYDA
                     YASFLDFVRSEIGLSATRCITLDLGDPSFIHTPERVERFIEEVTEALDMEAPVTTGG"
     gene            complement(1316..>2277)
                     /locus_tag="PK442_08615"
     CDS             complement(1316..>2277)
                     /locus_tag="PK442_08615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOO87752.1"
                     /translation="SFSPVDDSEGGFSAISLAATDLTESRARERECRAHADNFRRLFS
                     SSPEMLLISSVKEGRVLSCNRAFLSRMGFAETSVLGRTEDELGIYDGAGQRERLVKEL
                     DDAASVENYEVQLKSSSGDVFPGRVSVERIESEGEPALLSLFRDITNEKQAEECKQAA
                     TDMLSGIPSRSGFERILNTEIERAMRYRGNMSLILIDVDGFGALNETLGREAGDRVLK
                     DFCAAVKSRIRPTDFIGRWGGDEFAVLTPMSGRIAFQIAEAIRDMIYNYRFLPDRILS
                     SSMGVAEFRRSMDMNEFVKRAEDALAEAKKAGGNKAILAPFVP"
BASE COUNT          473 a          733 c          579 g          492 t
ORIGIN      
        1 ccctcgacaa tcgggcactt gcgttcacgg atcaagcgcc ccttgtcgat gatcagcacc
       61 tttttttcct gccgcacaca ctccatggcg gcaaagattc cggcaggtcc ggaccccact
      121 ataatgacat cgtacatcat gagacgcact tcctttcata gcgtcctctg caacgctccc
      181 ggactcgtcg gatgtgaccg cgtgcgaacc atcttccaaa agagaagagg gcattatctc
      241 ccgcttctgt catatttcga ctttcctctg acatttttcc ggagcttcct gagtatttca
      301 agattcctcg caagctcgtc ggcgttcctt tcgcctgcaa acgactcgac ctgtatcttt
      361 cccatcttca caagcaggtt gatcgccttc gtatctatcc ccagttcatc ggcaagctgc
      421 tgtatcgaga ttttccggtg tctgcccgcc tccgcagtgt tgtcccgcag atagccccat
      481 gcgagcatgt aaataccctg cacctcctga gcacacggag gacagagcgc tacctgcttc
      541 tccccgccct cgtcgaacca tgccttgccg caatactcgc atactctcaa cgccatagcg
      601 tcagcctccc gtcgttaccg gcgcctccat atccaatgct tccgtcactt cctcgatgaa
      661 acgctccact ctttcgggag tgtgaatgaa cgaaggatcg cccaaatcca gggttatgca
      721 gcgcgtcgcc gagagtccta tctccgagcg cacgaaatcg aggaaagacg catacgcgtc
      781 gtagagcgaa cgccagtact cctcgcccgc ctctatctcg tcggagcgcc cccgcatacg
      841 gatgcgccgc atgatctcct cgaacggaca ggagagcaca atgatcagct tgggcatggc
      901 cagatgcttc agaagcgaat cgtacagcat caggtacgtt ccgaactgac tgtcccggaa
      961 atagcccagc ttgtagtaaa gggatgcgta tatcttgtcg ccgaagacgc tgcggtcgag
     1021 aatatactcg tcggactccg acgcgcagag atactgggcg aacctcgtca cgaggaagtt
     1081 gagctgcata gggaaagccc agcgccgctc caggtggaaa cggcgcagca gatcgtactg
     1141 atcccggaac tcttccggca tgacgcgcag tccaagccgc tcggagagga gattcaccac
     1201 cgtcgatttg ccgctcgccg tcataccctc aacaataatc tgcaccctgc ccatattctt
     1261 cctcctccgg ttgtatcatc cccgacgttt ccggcgcgaa aagagcgacg ctacgtcagg
     1321 ggacgaaggg cgcgaggatc gctttgtttc cccccgcctt ttttgcctcg gcgagcgcgt
     1381 cctccgctct ttttacgaat tcattcatgt ccatgctccg acgaaactcg gcgaccccca
     1441 tgctggagga gagtattcta tcgggaagga atctgtagtt gtagatcatg tcgcgtatcg
     1501 cctccgcaat ctggaaggcg atacgtccgc tcatcggcgt cagtacggcg aattcgtccc
     1561 cgccccagcg tccgatgaaa tcagtgggac ggatgcggct cttgacggcg gcgcagaagt
     1621 ccttgaggac tctgtcgccg gcttcccgcc cgagtgtttc gttcagcgcg ccgaagccgt
     1681 ccacgtctat cagaatgagc gacatgttcc ccctgtaccg catcgcccgt tcgatctccg
     1741 tattcagaat acgttcgaac ccgctgcggc tgggaatccc ggagagcata tccgtggcgg
     1801 cctgtttaca ctcttcagcc tgtttttcat tcgtaatatc ccggaaaaga gaaagaagcg
     1861 caggctcccc ttccgattcg atcctctcga cggagacacg ccctggaaac acatcgccgg
     1921 aggacgactt cagctgcacc tcgtaatttt cgacagacgc cgcgtcgtcc aattctttca
     1981 ccagccgctc tctctgcccc gctccgtcgt agatgcccag ctcatcctcg gtacgcccca
     2041 acaccgaggt ttcggcgaat cccatccggg agaggaacgc acggttgcag ctgagcacgc
     2101 gcccctcctt caccgaggag atcagcagca tctcgggaga ggaggagaac aagcgccgga
     2161 agttgtccgc atgggcgcgg cactcccgct cccgggcacg ggattcggtc aggtcggtcg
     2221 ctgcgagtga aatggctgaa aaaccgcctt ccgagtcatc taccggtgaa aaggaga
//
LOCUS       DAOSWW010000418         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_38000_length_2272_cov_3.352729, whole genome shotgun sequence.
ACCESSION   DAOSWW010000418 DAOSWW010000000
VERSION     DAOSWW010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38000_length_2272_cov_3.352729"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1429)
                     /locus_tag="PK442_08620"
     CDS             complement(137..1429)
                     /locus_tag="PK442_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOO87753.1"
                     /translation="MDRDTVDSVRRLAIDGPLLLQQIGLLGEVGKEPDGGRTRLALSG
                     QDKRARDLLVQWMKELGLDVKIDPIGNIFGILYGKGDSCADALMIGSHIDTVINAGPF
                     DGVYGVLSGLAVARAFRNAGVRPLRPLIVAAFTNEEGVRFQPDMMGSLFFTGGIPIDD
                     ALETKGIDGALLGQELRRIGYKGETEPSSLFPAEYLELHVEQGPVLDSKELDIGVVDN
                     LQGISWKRIEISGTANHAGTTPVSMRRDAGVAAARIIVFLRELALSSEGTRTTVGSLR
                     FYPDLINVIPSRAVLTVDIRDPEGARLAEGEKTLLRFLEELRTKEGVEISTESLARFE
                     PVLFDEELASKIEEAAKKRGFSSMRITSGAGHDAQMLARVAKAAMIFVPSRGGISHNP
                     VEYTPDKQLLQGVHVLLDVAAGRLLKEGNKTEVEQKSL"
     gene            complement(1626..>2272)
                     /locus_tag="PK442_08625"
     CDS             complement(1626..>2272)
                     /locus_tag="PK442_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOO87754.1"
                     /translation="CQVTVAPNSNPYRVAAVRISPETLVVEKGRPVSLTATVVPETAT
                     NKNVTWRTSNSYVASVSSQGVVNGLAQGHTVVSVRSDDGGFVAECSVDVVDYEIRVNG
                     ISLNHTTTDVNVDGTRYLVASISPANATNKRVRWVTSNWEIASVSQNGIVVGVRKGRA
                     IISAITEDGGFVAECLVSVNEGWLDFIEGCSALNSSPFSLLLLIPLLSLIGRRK"
BASE COUNT          532 a          692 c          541 g          507 t
ORIGIN      
        1 ggcgcgaagc cgtcctattt cggaagtaac gctgaccttc actctatctc cctcctgcgg
       61 gtggatacaa agtcagagat aagaacatgc gttcgttcgt atttcccgga atcagatgct
      121 tgaaaaacat gaagccctac agactcttct gctcaacctc cgtcttgttt ccttccttga
      181 gaagacgccc ggccgcgacg tcgagcagga catgaacacc ctgaaggagc tgcttgtccg
      241 gagtgtactc cacaggattg tgacttatgc cgcctctgct gggcacaaag atcatcgccg
      301 cttttgcgac ccttgcaagc atctgggcgt cgtgtccggc gcccgatgtg atgcgcatcg
      361 aagaaaaacc ccgctttttt gccgcttcct cgatttttga cgcgagttcc tcgtcgaaaa
      421 gcaccggttc gaaacgcgca agactctccg tggatatttc caccccctcc ttcgtccgga
      481 gttcctccag aaaacgaaga agtgttttct ccccttcggc aagacgggcg ccttccggat
      541 cgcggatatc cacagtaaga acagccctcg atgggatcac attgataagg tcggggtaaa
      601 aacgcagcga cccgacggtc gtccgcgtcc cttcggatga aagcgcaagc tccctgagga
      661 agacaatgat tcgagcggcg gcaacccctg catcccggcg catcgaaacc ggagttgttc
      721 cggcatggtt cgccgttccg gagatctcga tccgcttcca ggaaataccc tgcagattgt
      781 ctacaactcc tatatccaat tccttgctgt cgagtaccgg cccctgttcc acatgcagtt
      841 cgaggtattc cgcagggaaa agagacgacg gttccgtttc ccccttatac ccaatacggc
      901 gcagttcttg acccagaagc gccccgtcga ttcccttcgt ttcaagagca tcgtcgatcg
      961 gtatgccgcc cgtgaaaaag agcgacccca tcatatccgg ctgaaaacgt acgccctcct
     1021 cgttggtgaa cgccgcgacg atgagcggac gaagaggacg cacaccggca tttctgaacg
     1081 ccctggcaac tgccagtccg gaaaggacgc cgtacacccc gtcgaacggt cccgcgttga
     1141 tcacagtgtc aatgtgcgat ccgatcatca gggcatcagc gcaggaatca ccttttccgt
     1201 aaagaatccc gaagatgttc cctattgggt cgattttcac atcaagtcca agttccttca
     1261 tccattgcac taacagatca cgtgcgcgct tgtcctgacc cgagagcgct aagcgagtcc
     1321 ttccgccgtc cggttccttt ccgacctcgc caagaagccc aatctgttga agaagcagcg
     1381 gcccgtcgat cgcaaggcgc cgtacgctat cgactgtatc tctgtccacg gaatttcctc
     1441 cttgttcccc ggatcaagag gaatactctt cggggtctca ttgtgttcca cagtatacac
     1501 taaaacttcc tggagaacac actcttttca cactgcatcc atagacgctc caagagagta
     1561 caaagaaggc cttcgctctt cgcgtttctt ttgcgaagag cgaaggcctt gttctcaaag
     1621 ttacgttact tccgtcgacc gatcagcgag agaaggggaa tcaaaagcaa cagagaaaaa
     1681 ggcgacgagt tcaaggccga acagccttcg ataaaatcca gccatccctc attcacgctc
     1741 acgaggcact ccgcgacaaa accgccgtct tcggtaatcg cgctgataat cgcccttccc
     1801 tttcgtactc ccacaacaat tccgttctgg gagacggacg ctatttccca gttcgacgta
     1861 acccagcgaa cccgtttatt cgtcgcattc gcaggggata tagaggcgac aagataccga
     1921 gttccgtcga cattaacatc tgttgttgtg tggttcagag atatgccgtt caccctgatc
     1981 tcgtagtcga caacatcgac ggaacactcc gcaacaaaac ctccgtcatc cgaacgaaca
     2041 ctcaccacag tatgcccctg agccagcccg ttgacaactc cctgcgaaga gactgacgcg
     2101 acataagaat tcgacgtcct ccatgtcaca ttcttgttcg ttgccgtctc tggtacaaca
     2161 gtagccgtca gagaaacggg tctccctttt tcgacaacga gagtctccgg tgaaatacgg
     2221 acggctgcca ccctgtaagg atttgaattc ggggccacgg tcacctggca cg
//
LOCUS       DAOSWW010000419         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_38002_length_2272_cov_3.101488, whole genome shotgun sequence.
ACCESSION   DAOSWW010000419 DAOSWW010000000
VERSION     DAOSWW010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38002_length_2272_cov_3.101488"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..1333
                     /locus_tag="PK442_08630"
     CDS             452..1333
                     /locus_tag="PK442_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOO87755.1"
                     /translation="MKHQPSPSILQRILQFQRDELTSSILYEYIAGRQKDEGNRTALL
                     EIAKAERSHYTTWKGYTGQELRPNMLKIFFYKIIHMLLGDTFTIKLFEIGETFGAQEL
                     REIEGEIPEARTIMKEEEEHEDRLMEMVDEERLHYIGSMVLGLNDALVELTGTIAGLT
                     FALMNTRLVALSGIITGVSATLSMAASNYLAERADGNAKALKSSFYTGMAYLVTVALM
                     VLPYLLLPNSLYLAAFAILLAVVVMIIAVFNYYVSVVQSIPFWPRFAEMGTISLSVAA
                     ISFVIGLLAKHLLGIEI"
     gene            1415..>2272
                     /locus_tag="PK442_08635"
     CDS             1415..>2272
                     /locus_tag="PK442_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO87756.1"
                     /translation="MHAVDAFRDKIVFERVTPERAASFADFPERMSPEIRRYLRSHGI
                     DALYSHQAEMFDRAMDGENTVVTTSTASGKTLAFLLPILQAILEDSRTRAIFLYPTKA
                     LASDQFRALSPLLEHFGAGRVQAGIYDGDTPPNERSRIRGSANIILTNPEMLNATFLP
                     NHGSFGFNFLFANLKYVVVDELHSYRGAFGSHLANLMKRLARICRYYGSSPRFLCSSA
                     TIANPVELAQKICGASFSLVSKDGSPAPEKRYYMWQPPLVRGGGFRISPAREATGLIA
                     QLVLRKSAFL"
BASE COUNT          503 a          689 c          625 g          455 t
ORIGIN      
        1 acaagcaaga aataccgagt aacctttcgc attcaacgca ctcctctcgg aataaaacga
       61 tctgttccgg ttgcagtcaa ccggcccggc gtccttggag cattcaagct ccggcgccat
      121 tcccaccctt ctccggactg agacagaccg cgttcacccc ctcaagagtt ccgtatcgga
      181 cgttttcccg tctctccgga tgtttcaaga aaacccggct gcatattttc aggagacttc
      241 atttcacttc gcaagacgat attttcctca ttgtagcagc gtgtacaacc gaagtccaca
      301 cccgttcggc aaaaatgttc ccaaattcac aacgtcgtcc gccgagcgcc aaagacgttt
      361 cgacataaat ttcacatggt ataataaaaa gttacatact atcgcaaaat cctcaagctc
      421 tctgcagaaa atgtgaaagg caggcaaaaa tatgaaacat caaccgagtc catccatact
      481 tcaacggatc ctacagttcc agcgcgacga actgacctcc agcatcctgt acgagtatat
      541 cgccggacgt cagaaggacg agggtaacag gaccgccctg ctggaaatag cgaaggcgga
      601 acggagtcat tacacaacgt ggaaagggta cacgggacag gaactccgcc cgaacatgct
      661 caaaattttc ttctataaaa ttatacacat gctcctgggc gacaccttca ccatcaagct
      721 ttttgaaatt ggagagacct tcggggcgca ggagctgcgc gaaatcgaag gggaaattcc
      781 agaagcgcgg accattatga aggaagagga ggagcacgag gaccgcctca tggagatggt
      841 ggacgaagaa cggctgcact acatcggctc catggtgctg gggttgaacg acgcgctcgt
      901 ggagctcacg ggcacgatcg ccggactgac cttcgcgctg atgaacaccc ggctcgtcgc
      961 tctttcggga atcatcacgg gcgtctcggc gacgctctcg atggcggcgt cgaactacct
     1021 ggccgaacgc gccgacggaa acgcgaaggc cctcaagtcg agcttctata ccggaatggc
     1081 ctatctggtc accgtcgcgc tgatggtgct cccctacctg ctgctgccca acagtctcta
     1141 cctggcggcg ttcgccatcc tgctggctgt ggtggtgatg atcatcgccg tgttcaacta
     1201 ctacgtttcg gtagtgcagt caatcccctt ctggccgcgc ttcgcggaga tgggaaccat
     1261 cagcctgagc gtcgcggcga tctccttcgt aatcggcctg ctcgcgaaac acctgctcgg
     1321 gatagagata taaacaaact caacgtgtca cgctccaagc gcaaaaagtt cagactaccg
     1381 gaggctatct tcttatgaaa cgtggtgaat aggtatgcac gccgtcgatg cgttcaggga
     1441 caaaattgtt ttcgagaggg tgacgccgga gcgcgccgcg tctttcgcgg actttccgga
     1501 gcggatgtcg cccgaaatcc ggcgatacct ccggtcgcac gggatcgacg cgctctactc
     1561 gcaccaggcg gagatgttcg accgtgcgat ggacggcgaa aacacggtcg tcaccacgtc
     1621 cacagcgagc gggaagacgc tggcctttct gctgccgatt ctgcaggcga tcctggagga
     1681 ttcgaggacg cgggcgatct ttttataccc caccaaggcg ctggcgagcg accagttccg
     1741 cgccctatcg cccctgctgg agcacttcgg cgcggggcgc gtccaggcgg gaatctacga
     1801 cggcgacacg ccgccgaacg agcgaagccg catccgcggc agcgcgaaca tcatcctgac
     1861 caacccggag atgctgaacg cgaccttcct gccgaaccac ggctcgttcg gcttcaactt
     1921 tctgttcgcg aacctgaagt acgtcgtggt ggacgagttg cactcctaca ggggggcgtt
     1981 cgggtcgcac ctcgccaacc tgatgaagcg gctcgccagg atatgccgct actacggctc
     2041 ctcgccgcgc tttttgtgca gctccgcgac gatcgcgaac cccgtcgagc ttgcgcagaa
     2101 gatttgcggc gcctccttct cgctggtctc gaaggacggc tctcccgcgc ccgagaagcg
     2161 ctactatatg tggcagccgc ctctcgtgcg gggaggaggc ttccgaatct ctccggcccg
     2221 cgaggcgacg gggctcatcg cgcagctcgt cctgcggaag tccgcttttc tg
//
LOCUS       DAOSWW010000420         2259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_38340_length_2259_cov_2.988203, whole genome shotgun sequence.
ACCESSION   DAOSWW010000420 DAOSWW010000000
VERSION     DAOSWW010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38340_length_2259_cov_2.988203"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PK442_08640"
     CDS             complement(<1..693)
                     /locus_tag="PK442_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO87757.1"
                     /translation="MLLMMIGVMCFFLVLGFPMIIPLIIAPIAVAAVYYPALDPMNLV
                     QQMLVGVRPLSLVAVPMFIFAADVISTGQVARRLIDFVMAFFGHTRGGLAVTTTAACT
                     FFGAVSGSTQATVVAIGGTMRPYLLRDGYDDSFSIALIINAAGIALLIPPSISMIIYG
                     VVTGSSVGELFIAGIGPGLLVFLLFSLYCSFAARKMDIVRTPKMPLPERVKALRKAIV
                     PIGFPLITLGGIY"
     gene            complement(695..1261)
                     /locus_tag="PK442_08645"
     CDS             complement(695..1261)
                     /locus_tag="PK442_08645"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO87758.1"
                     /translation="MKKLELKGLEKIDAVLTWVEERILVFSILFMAVILIANVISRNF
                     FNSSINASEEISQFLVVLATFLGTSYAARKGLHIRMSILNDFLKGKPQKLLALFVSLA
                     TAVIMFYISWLAFRYVRRVGTLHRVSPILQVPLHYIWSVVPVGFLLTGVQYLLTFARN
                     LTSGEVWVSYYVKDENAEPEAAPAEGGN"
     gene            complement(1355..2257)
                     /locus_tag="PK442_08650"
     CDS             complement(1355..2257)
                     /locus_tag="PK442_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87759.1"
                     /translation="MPGRDGWELPASKKSFPDGANYRIEIAGVERYSNMVAMVDEAKK
                     RKIPIHRAICTVSGSTYCDFDELKNMAKLARDEQIEMIMVVGHRKAWDVGSKEMGYPE
                     GGPQGPRHRGSDSMAYWVEDMMRNVEAGVRGFLVYDEGALKLVNQMRAEGFLPKETIF
                     KWSVFGGQCSPAGAKLLRELGADTMNPISDVSLPILSSIRSTVDMPLDVYMIIVDSFG
                     GMFRAYDAPEIVRVASPVYFKIEPGTSEGDIYKPWVTDSWHDEFIRAKVKIAATLREI
                     MERRAPELKLSEQGPADLRLPVVD"
BASE COUNT          423 a          682 c          759 g          395 t
ORIGIN      
        1 gtagatgccg ccgagggtga tcagcgggaa gccgatgggg acgatcgcct tccgcagcgc
       61 cttcacccgc tcggggagcg gcatcttcgg cgtacggacg atgtccatct tccgcgccgc
      121 gaagctgcag tagagcgaga agaggaggaa gacgagcagc cctgggccga tgccggcgat
      181 gaagagttcg ccgacggagc ttcccgtgac cacgccgtag atgatcatcg agatgctcgg
      241 ggggatgagc agcgcgattc ccgccgcgtt gatgatgagc gcgatggaga acgagtcgtc
      301 gtacccgtcg cggagcaggt aggggcgcat ggtgccgccg atggcgacga ccgtggcctg
      361 cgtggagccg gagaccgcgc cgaagaaggt gcacgccgcc gtggtggtga cggcaagacc
      421 gccgcgcgtg tggccgaaga aggccatgac gaagtcgatg agccgccgcg ccacctgccc
      481 ggtggagatg acgtccgcgg cgaagatgaa catcgggacg gcgaccagcg agagcggccg
      541 gacgccgacg agcatctgct ggacgaggtt catcgggtcg agcgccgggt agtagaccgc
      601 ggccaccgct atgggggcga tgatcagggg gatgatcatc gggaagccga ggacaaggaa
      661 gaagcacata acgccgatca tcatgaggag catctcagtt tcccccctct gccggagccg
      721 cctcgggctc cgcgttctcg tccttcacgt agtaggagac ccagacctcg ccgctcgtca
      781 ggtttctggc gaaggtcagg aggtactgca cgccggtgag gaggaagccg acgggaacca
      841 ccgaccagat gtagtggaga ggaacctgga gtatggggct gacgcgatgg agcgtcccca
      901 ccctgcgcac gtagcggaag gcgagccacg agatgtagaa catgatgacg gccgttgcca
      961 gagagacgaa gagggcgagc agcttctgcg gcttcccctt cagaaagtcg ttcaggatgg
     1021 acatccgtat gtggagcccc ttcctggcgg cgtagcttgt ccccaggaag gtcgccagca
     1081 ccacgagaaa ctggctgatc tcctccgacg cattgatgct ggagttgaag aagttgcgcg
     1141 agatcacgtt ggcgatcagt atgaccgcca tgaacagaat gctgaacacc agaatccgtt
     1201 cttctaccca tgtcagcacg gcgtcgatct tttcgagtcc cttcaattcg agtttcttca
     1261 atggctgatc ctcccttcag gtgagcgggc gcggcccgga ggccgaaggt aaaacagccc
     1321 gggaagcgga tactcccggg ctgttcggta gatgtcagtc gacgacgggc aggcggaggt
     1381 cggcgggccc ctgttcggag agcttcagtt cgggggcgcg gcgttccatg atctcgcgga
     1441 gcgtcgcggc gatcttcacc ttggcgcgga tgaactcgtc gtgccacgag tcggtcaccc
     1501 acggcttgta gatgtcgccc tcggaggttc ccggctcgat cttgaagtag acgggggagg
     1561 ccacgcggac gatctcgggc gcgtcgtagg cgcggaacat gccgccgaag gagtcgacga
     1621 tgatcatgta cacgtcgagc ggcatatcga cggtgctgcg gatgctggag aggatcggga
     1681 gcgagacgtc ggagatcggg ttcatcgtgt cggcccccag ctcccggagg agcttcgccc
     1741 cggcggggct gcactgaccg ccgaaaacgg accacttgaa aatggtctcc ttcgggagga
     1801 agccctcggc gcgcatctgg ttcaccagct tcaacgcccc ttcgtcgtag acgaggaagc
     1861 cgcggactcc ggcctccacg ttccgcatca tgtcctcgac ccagtaggcc atgctgtcgg
     1921 agcccctgtg cctcggcccc tgcgggccgc cctccgggta ccccatctcc ttggagccga
     1981 cgtcccacgc cttgcggtga ccgaccacca tgatcatctc gatctgctcg tcgcgcgcaa
     2041 gcttcgccat gttcttcagc tcgtcgaagt cgcaataggt cgagccgctc accgtgcaga
     2101 tggccctgtg gatgggaatt ttgcgcttct tcgcttcgtc caccatggcg accatgttgg
     2161 agtagcgctc cactccggcg atctcgatgc ggtagttcgc cccgtcgggg aagctcttct
     2221 tcgaggcggg cagctcccat ccgtcccttc ccggaatcc
//
LOCUS       DAOSWW010000421         2257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_38398_length_2257_cov_3.808356, whole genome shotgun sequence.
ACCESSION   DAOSWW010000421 DAOSWW010000000
VERSION     DAOSWW010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38398_length_2257_cov_3.808356"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1153)
                     /locus_tag="PK442_08655"
     CDS             complement(152..1153)
                     /locus_tag="PK442_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HOO87760.1"
                     /translation="MFLMLLFVLTLLLLLAASPSEAKTHSFFITKEQAYNGMLRSDIP
                     PALTIEDGDTVVFNTVMLMAGELSPEMTFDEMFALRTRMKERGLGVYAFTGPFFVKGA
                     EPGDVLEVRIKRIVPGKYAVTHIYPDETGIGGLPEGFETGYLKPLYLSEDRKSIPFAP
                     GVTVPVRPFLGTMAVAPRPGEEHPPAVPGYFAGNMDNKELGAGSTLYIPVNVPGALFM
                     AADAHAVQGDGEVSIAAAETYFEEVELEFVVRKDLPLRFPMAETPDHWIVMAFHKDLD
                     EAMKGAIREAIRFLEREKRLTRNEAYSLCSLAVDFRVTQVVDGDKGVHGMIPKAIFG"
     gene            complement(1341..1757)
                     /locus_tag="PK442_08660"
     CDS             complement(1341..1757)
                     /locus_tag="PK442_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOO87761.1"
                     /translation="MSRTKIFIDTNILIYTVDAHDAEKQIRARSIVKSVVENDLPVIS
                     TQILQEFYSASTTKLKVDPLIAKNLVHNFGNMEVVPVDPHTIEQGIDISILCRISFWD
                     GLVVAAAEQGNCEILFSEDLNNGQTIRGIKILNPFF"
     gene            complement(1750..1974)
                     /locus_tag="PK442_08665"
     CDS             complement(1750..1974)
                     /locus_tag="PK442_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87762.1"
                     /translation="MRNITLSLDKETLRRGREYAKRHNISFNALVRRLVEQTVASSSS
                     QWLEDTFALMDQTPVSSQGEKWSREELHRV"
     gene            complement(2054..>2257)
                     /locus_tag="PK442_08670"
     CDS             complement(2054..>2257)
                     /locus_tag="PK442_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOO87763.1"
                     /translation="VFSAVVEETAPLGGSIRLYLASENGVLWQAEHPSESYQTLRLRP
                     GSKVRFAVPPAGIESWSGGPSPR"
BASE COUNT          468 a          643 c          664 g          482 t
ORIGIN      
        1 gatcatgctt gttttggcag cgctctttgt cgccaaggag atattcttct agcgccgcgc
       61 cggccgatca ggcacagtac actacgcgaa ggggagcgct ccgaaccgca gggcgctccc
      121 ctttttcatg ggctgtccgt gccggtgtcg cttacccgaa aattgccttc gggatcatgc
      181 cgtgcactcc cttatctccg tctactacct gggtaacccg gaagtcgacc gccaaactgc
      241 acagggagta ggcttcattc cgggtgagcc gcttctcccg ttcgaggaag cggatcgcct
      301 cgcgaatggc ccccttcatc gcttcgtcga ggtctttatg gaaggccatc acgatccagt
      361 ggtccggcgt ctccgccatc ggaaagcgca gcggcaggtc cttgcggacg acgaattcca
      421 gctcgacctc ctcgaagtag gtctccgccg cggcgatgct cacctcgccg tccccctgca
      481 cggcgtgcgc gtcggcggcc atgaagagcg cccccggcac gttcaccggg atatagagcg
      541 tgcttcccgc ccccagctcc ttgttgtcca tgttccccgc gaagtagccc gggactgcgg
      601 gagggtgttc ctcgccgggg cgcggggcca ccgccatcgt cccgaggaag ggacggacgg
      661 gaaccgtcac gccgggagcg aaggggatgc ttttccggtc ctccgagagg tacagcggtt
      721 tgagataccc ggtctcgaac ccctctggaa ggccgccgat ccccgtttcg tcagggtaga
      781 tgtgcgtgac ggcgtacttg ccggggacga tgcgcttgat acggacttcg aggacgtctc
      841 ccggctcggc ccccttgacg aagaacggcc cggtgaaggc gtacacgccc aggccgcgct
      901 ctttcatgcg cgtgcgaagg gcgaacatct catcgaacgt catctcgggc gacagctctc
      961 cggccatcag catcaccgtg ttgaaaacga ccgtgtcgcc gtcctctatc gtgagcgcgg
     1021 gggggatatc gctccggagc atgccgttgt acgcctgttc cttcgtgatg aagaaggagt
     1081 gcgtcttcgc ctcggaggga gaagcggcca ggagcagcag aagcgtcagt acaaaaagca
     1141 gcattaaaaa catgcgtgtt cctcctcgat gtgaaagtct gaagaaagta tatcatccgg
     1201 ggagtgaacg cgccgtcagc ttcttttctc gggtgagagg gcgcgaaaat tctcccgaac
     1261 ggagagcgga gaagttcgta cggcggcttc cgttcggtac gcgacgttga tgcaagcggg
     1321 ctttcgggct gtgaaaaagg ttaaaaaaac gggttgagga tctttattcc ccgtattgtt
     1381 tggccattat tcaggtcttc cgaaaagaga atctcgcaat tgccctgctc tgccgctgca
     1441 acgacaagtc cgtcccagaa ggaaatccga cagagaatac tgatgtcgat accttgttcg
     1501 atcgtgtgcg gatcaacagg gacaacctcc atgttgccga agttatgaac gaggtttttg
     1561 gcaataagag gatcaacctt caacttggtt gtagaggcgc tgtaaaactc ctgcaaaatc
     1621 tgtgtggaaa tcacaggaag atcattctcc acaacgcttt tcactatgga acgggcccga
     1681 atctgttttt cggcgtcgtg agcgtcaacc gtatatatca gaatgttggt gtcgatgaat
     1741 attttggttc tagacacgat gcagctcttc cctcgaccat ttctcacctt gcgacgagac
     1801 gggcgtttga tccatgagag cgaatgtgtc ttccagccac tgggaagaag aggacgccac
     1861 cgtttgctcg accaaacgac gtacgagcgc gttaaaagaa atattatggc gtttcgcgta
     1921 ctctcttccc cgtcggagcg tctctttatc gagagaaagc gttatattac gcatgattcc
     1981 acctcctatt cacacataat acgtgcgcac ttacgtgctg tcaagatatc gaatggaaga
     2041 gtatgcagtt gcattatcga ggagaaggcc ccccgctcca cgactcgatg cctgccggag
     2101 ggacggcgaa acgcaccttc gagcccgggc gaagccggag ggtctggtac gactcggagg
     2161 ggtgttcggc ctgccagagg acgccgttct ccgaggcgag atagaggcgg atgctcccgc
     2221 ctagcggcgc ggtctcctcg acgacggcgg agaagac
//
LOCUS       DAOSWW010000422         2245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_38740_length_2245_cov_2.667580, whole genome shotgun sequence.
ACCESSION   DAOSWW010000422 DAOSWW010000000
VERSION     DAOSWW010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38740_length_2245_cov_2.667580"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /gene="pilM"
                     /locus_tag="PK442_08675"
     CDS             <1..793
                     /gene="pilM"
                     /locus_tag="PK442_08675"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOO87764.1"
                     /translation="KNSLADAGDLLAALQALGSSIGGFKVPVALSIPSRDILIRVIEM
                     PELEMNDAREALQWDFEKYFPYAFSDAAVDISKVENPLKGEPGTMSVLVAACRLRTVE
                     SVMRLAATAGMQLSTIEPENVAMFRAALGPTLSFPGGYLAVFAENGVSQLILGYKDNG
                     VLYRTSLIDINTVEEEQKDFTALVREIGNTLTFARNQYKELQVEHIMLGGHFVGEAGL
                     KDVIEETTGLKVLVSDPWSPWGIPVPSDDALGWETAVGLAVRELS"
     gene            790..1389
                     /locus_tag="PK442_08680"
     CDS             790..1389
                     /locus_tag="PK442_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOO87765.1"
                     /translation="MKVSLDLRPRALVESQKKRLNVSRLFMTLFLLSFLLIGGSTLFW
                     AFWNYRSLVDRVAFLEDTLSIQRAQNARLANELRRLGEEEAVYVAALKLLQDELPALE
                     FLQAMETSLPPGVWVRTLSIVPGKVSLQGSAYKEDDVVEFGKGLLDAPVVAAVDFPVT
                     TRVVRDGQSLVDYTLACSLRDFAATIPPAVPVEKEAESK"
     gene            1386..1880
                     /locus_tag="PK442_08685"
     CDS             1386..1880
                     /locus_tag="PK442_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87766.1"
                     /translation="MTRKDTLIFLILLLVSGGMLFGAFHLIKQMEQLRVTEKELRTQG
                     MEIELQSAERMRQIEVFKRAIAQLERYQIQIPENEVDFYSWVQQELTRNGVRSNVVKP
                     GTSPQGRRAVQVDFQGPYYSFVQTISDWRNLKVALRVSSVRMDSVDGDNAKGVAVVES
                     VLKK"
     gene            1956..>2245
                     /locus_tag="PK442_08690"
     CDS             1956..>2245
                     /locus_tag="PK442_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87767.1"
                     /translation="MPRALLFLIFFGGTIFFGWSFKRVVDLSVEESVFVQPPPRQSQE
                     DAKRLDGILESYRGTVEARQGSKGLVATVTEKNRRPLAVSRRSTKGAGGAAAG"
BASE COUNT          410 a          610 c          722 g          503 t
ORIGIN      
        1 caaaaactcc cttgccgacg ccggcgacct tcttgccgcg cttcaggcgt tggggtcgag
       61 tatcggcggg ttcaaggttc cggtggccct ttccatccct tcgcgcgata tcctgatccg
      121 cgtcatcgag atgcccgagc ttgagatgaa cgatgcccgc gaggcgctgc agtgggactt
      181 cgagaagtac ttcccgtacg ctttttccga tgcggcggtg gacatctcga aggtcgagaa
      241 ccctctcaag ggcgagccgg ggacgatgtc cgttctggtg gcggcgtgca ggcttcgtac
      301 cgtggagtct gtgatgcgtc ttgcggctac ggcgggaatg cagctttcga ccatcgagcc
      361 ggagaacgtc gcgatgttcc gcgcggcgct ggggccgacg ctctcgttcc ccggaggata
      421 tctggcggtc tttgccgaaa acggcgtgag ccagcttatc cttgggtaca aagataacgg
      481 cgttctgtac aggacgtcgc tgatcgacat caacacggtc gaggaggagc agaaagactt
      541 taccgcgctt gtgcgcgaga tcggcaacac gcttacgttc gcacgaaacc agtacaagga
      601 gcttcaggtg gagcacatta tgctgggagg ccatttcgtc ggtgaagcag ggttgaagga
      661 cgtcattgag gaaacgaccg gactgaaggt tcttgtttcg gatccgtgga gtccgtgggg
      721 aatcccggtt ccctccgacg atgcgcttgg ctgggagacc gccgtaggcc tggcggtgcg
      781 tgaactgtca tgaaggtatc tcttgatctc cgtccgcgcg ctcttgtcga gtcgcagaag
      841 aagcgcctga atgtctcccg tctttttatg acgctgttcc ttctctcgtt ccttcttatc
      901 ggcggctcga cgctcttctg ggctttttgg aactatcgtt ccctggttga tcgcgtcgct
      961 tttttggagg acacgctttc aatccagcgc gcgcagaacg cgcgccttgc gaacgagttg
     1021 cgccgcctcg gcgaggaaga ggctgtgtat gtggctgcgc tcaagctgtt gcaggacgag
     1081 ctccccgcgc tcgagttcct gcaggcaatg gaaacctcgt tgccgccggg cgtctgggtt
     1141 cgaacgctct cgatcgttcc cgggaaggtc tcgcttcaag gaagcgccta caaggaggac
     1201 gacgtcgtcg agttcggcaa ggggcttttg gacgcccctg tcgtcgctgc ggttgatttc
     1261 cctgtgacaa cgcgggttgt acgggacgga cagtcgctcg tcgactacac gctcgcctgt
     1321 tcgctgcgtg atttcgcggc gacgatccct cctgcggttc ccgtcgaaaa ggaggctgaa
     1381 tccaaatgac ccgcaaggat acgctcattt ttctcattct tcttcttgtg tcagggggga
     1441 tgctcttcgg cgcgttccat ttgatcaagc agatggaaca gcttcgagtc acggagaagg
     1501 agctgcggac tcagggaatg gagattgagc ttcagtccgc cgaacggatg cgccagatag
     1561 aggtgttcaa acgggcgatc gcgcagcttg aacgatatca gatccagatt cccgagaacg
     1621 aggttgattt ttactcctgg gttcagcagg aattgacgcg caacggagtc cggagcaacg
     1681 tggtgaagcc ggggacttcc ccgcagggac gccgcgccgt tcaggtggat ttccagggcc
     1741 cgtactattc gtttgtgcag acgatctccg attggcgcaa cctgaaggtc gcgctcaggg
     1801 tttcctccgt gcgaatggac tccgtcgacg gcgacaacgc taaaggcgtg gccgtcgtcg
     1861 aatccgtgtt gaagaagtag gtggtggaaa tggtcgaccc agcagtcaac gaatcgttct
     1921 agggaaagct tgtcgccggt gaaggcgcct tccgtatgcc ccgcgcgctc ttgttcctta
     1981 tatttttcgg cgggacgatc tttttcggat ggtcttttaa acgggtagtt gatctttccg
     2041 tggaggaatc cgtttttgtc cagcctccgc ccaggcagtc gcaggaggat gcaaaaaggc
     2101 tggacggcat ccttgaaagc taccgcggca cggtggaggc caggcagggg agcaaggggc
     2161 tggttgcgac cgtgacggag aagaaccgcc gtccgctcgc ggtttcccgc aggtcgacaa
     2221 agggggcagg cggggcggcg gccgg
//
LOCUS       DAOSWW010000423         2243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_38797_length_2243_cov_2.805302, whole genome shotgun sequence.
ACCESSION   DAOSWW010000423 DAOSWW010000000
VERSION     DAOSWW010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38797_length_2243_cov_2.805302"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="PK442_08695"
     CDS             complement(<1..1815)
                     /locus_tag="PK442_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87768.1"
                     /translation="MFFDIRGIVLFWLVVNLMLTVTNIAMYFRYRKRFEGLLFWSVHM
                     ILQVTGMTFIFLRGFIPGFVSVFVANGMLAMGALSLLVGLELFVDKSRRHGFNLAAIA
                     GYLAVMAYFYWIRPDLAVRTVATSALHAAVAFQICALLLRRVPCGLRDITRITGLVAG
                     VYAAVHFSRMCLLLFFSPQTNDLFRSGFVDSFFLACILTLEIALKLAIIMMLSRRLLE
                     TLEQTTAEAERMAVAAQTANRAKSEFLANMSHEIRTPMNGVLGMTGLLLDMRLTDEQR
                     SCAEAVRSSGEHLLGILNDILDFSKIEAGRLDLEDVDFDLRAVLDEIASILGARAGAK
                     GLQLRLSMDPGVPASLRGDPLRLRQVLNNLADNAVKFTSRGEVSISVESEEEGQNDVA
                     LRFSVRDTGIGIPLEKQGALFDKFSQVDTSTTRKYGGTGLGLAISKKLVEMMGGEIGV
                     TSAEGRGSEFWFSARFGKASGAGRALPQKEHRDMTPLSAGQRRARLLVAEDNTTNLQV
                     ALGILKGLGLDADAASDGEEAVEAVQRHPYDLVLMDCQMPRMDGYEATRRIRSLEGDV
                     RDVPIIAMTAHAMAGDREKCLQAGMNDYVSKPISPRLFV"
BASE COUNT          465 a          717 c          651 g          410 t
ORIGIN      
        1 gacgaagaga cgaggagaaa tcggcttgga aacgtaatcg ttcattcccg cctgcaggca
       61 cttctccctg tccccggcca tcgcatgcgc cgtcatcgcg atgatcggga cgtcgcgaac
      121 gtcgccctcc agcgagcgaa tcctccgcgt cgcctcgtag ccgtccatcc ggggcatctg
      181 gcagtccatc agcaccaaat cgtacggatg gcgttgcacc gcctcgacag cctcctctcc
      241 gtccgaggcc gcgtccgcgt ccagtcccag gcctttcaga atgcccagcg cgacctggag
      301 gttggtcgtg ttgtcctccg cgacgagaag ccgcgctcgc ctttgccccg cggagagcgg
      361 cgtcatgtcg cggtgttcct tttgcggaag agcgcgcccc gcaccggacg ccttgccgaa
      421 ccgcgccgaa aaccagaact ccgatccccg gccttcggcg cttgttacgc cgatttctcc
      481 gcccatcatc tcgacgagtt ttttcgagat agcgaggcca aggccggtac ctccgtattt
      541 tcttgtcgtc gaggtatcca cctggctgaa cttgtcgaag agcgctccct gcttctccaa
      601 agggattccg atccccgtgt cgcgcaccga aaagcgcagc gcgacatcgt tttgtccttc
      661 ctcctcggac tctacggaga tcgaaacctc gccccgcgaa gtgaacttga ccgcattgtc
      721 cgccaggttg ttcagcacct gtcgaagtcg cagcgggtct ccccgcagcg aggccggcac
      781 gccgggatcc atggaaaggc gcagttgcag gcctttcgcc ccggcgcgcg cccccaggat
      841 ggaggcgatt tcatcgagca cggcgcgcag gtcgaaatcc acgtcctcca gatcaagccg
      901 cccggcctcg atcttcgaga agtccagaat atcgttgaga atgccgagga gatgctcgcc
      961 gctcgaacgc acggcctcgg cgcagctccg ctgctcgtcg gtcagacgca tatcgagcag
     1021 caaccccgtc atccccagaa ctccgttcat gggcgtccgt atctcgtggc tcatattcgc
     1081 caggaattcg ctcttcgccc tattcgcggt ctgagcggcc acggccatcc gctcagcctc
     1141 cgctgtggtc tgttcgaggg tttcgagaag gcggcgcgag agcatcatga tgatggcgag
     1201 cttcaaggct atttccagcg tgagaataca ggcgagaaaa aacgaatcga caaaaccgga
     1261 gcggaacaaa tcgttcgtct gtggcgagaa gaagagcaac agacacattc tggaaaaatg
     1321 gaccgcggcg tagacgcccg ccaccagtcc ggtgatgcgc gtgatgtccc gcagaccgca
     1381 aggaacccgc cggagcagca acgcgcagat ctgaaacgcg acggcggcat gaagagcgga
     1441 cgtcgcgacg gtgcgcaccg cgagatcggg acgaatccag tagaagtagg ccataaccgc
     1501 aagatagcct gcgatcgccg cgaggttgaa cccatgccgt cggctcttgt ccacaaacag
     1561 ttcgagcccc acgaggagcg agagcgcccc catggcgagc atcccgttgg cgacaaaaac
     1621 ggagacgaac ccggggatga accctcgaag aaagatgaag gtcatgcccg tcacctgaag
     1681 aatcatgtgc acggaccaga agaggaggcc ttcgaagcgc ttccggtagc ggaagtacat
     1741 ggcgatattc gttacggtga gcataagatt gacaaccaac cagaaaagca caatgcctct
     1801 gatatcgaaa aacatgggat ttccctcccg tacccgattc gcgaacgcgg ctcctcaact
     1861 catgatagtg cactttcgtt cctctcgcaa gcggcgctgc gggacggacg gaagattcgt
     1921 tataaaaaaa tcccccgtct tctcggagaa gcgggggaca aagcggcgct accgggtcgg
     1981 ggcatcgggt cggggcatcg gttcggggca agcgtgccgg ttcgttcggc gcacgctctg
     2041 tcgccgcacg ccggaggcgc agcgcggcgg aggtcagccg agatagattt tttgcagatc
     2101 gtccagcagg gcgtgaaact gctcgtgcct cccctttccg atccgctcct cctccagttt
     2161 ctcgaactcg cggaacagct cttcatcctg cgcatcgctc agacaccggc cggccatgac
     2221 gaagagcacg ttgtcttctt tct
//
LOCUS       DAOSWW010000424         2236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_38969_length_2236_cov_3.005502, whole genome shotgun sequence.
ACCESSION   DAOSWW010000424 DAOSWW010000000
VERSION     DAOSWW010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38969_length_2236_cov_3.005502"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PK442_08700"
     CDS             <1..551
                     /locus_tag="PK442_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87769.1"
                     /translation="EILHEDFRRPIALGDLLQYVFTQISPFDWRETVSLSAEEGILDE
                     YVTVNVVRLSRALVNLLENAHRANTLVNGSRIVMRISGTQEFAEIAVLDEGPGFRRTR
                     GEEDGKPLKAGGLSGMEWSSSSGWNSTGLGLQYVTMVVRNLGGKFVIEDLPDGGARTA
                     IVLEKNAAGKESSKAGREAFGM"
     gene            581..1225
                     /locus_tag="PK442_08705"
     CDS             581..1225
                     /locus_tag="PK442_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO87770.1"
                     /translation="MPLHIGAIDDDRSILYTLEAMASTEGWRMLTTNVPEECLDWIKL
                     DEVDILLVDYHMPVMNGLHLIRKARELSSKLVLIALTVEDGGDLAGQLVLAGADDFIT
                     KPIRLADFTARIRLHEKLFAHREQLNWEERKKGVSKDTMRKVLEKLKEFSSPKDCDDV
                     AAACGLAYVTAHRYLEYLAERGLIIRSAGVQDGRPGRPKTYYAVPVLSSNAASQ"
     gene            complement(1288..2031)
                     /locus_tag="PK442_08710"
     CDS             complement(1288..2031)
                     /locus_tag="PK442_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOO87771.1"
                     /translation="MKKILFALLALLLLGTAGWANVMAKDVLLVGTESTYPPYEFRDE
                     NNQLKGFDIEMMETIAAKIGKKIEWVDMPFDSLIPSLLAKKIDIVAAGMSATEERAKR
                     VAFSTNYEISISAFIVAAANDSMKVLGDMKGKTIAVQLGTVQETYSQSLEDVVVKSFQ
                     KFDDCVREVILGRVDATLMDTPVAKSYVEHKDFQGKIKIAFEQEITGSGKALAMNLGE
                     TELIEAVNKALAELEGSGELDALKKQWFK"
BASE COUNT          495 a          573 c          627 g          541 t
ORIGIN      
        1 ccgagattct ccacgaggat tttcgccgtc ctattgccct cggggatctt ctgcagtacg
       61 tcttcaccca gatcagcccg ttcgattggc gagagaccgt gtcgctctca gctgaagagg
      121 gaatattgga tgaatacgtg acggtgaatg tggtgcgtct ttccagagcg ttggtgaatc
      181 tgctggagaa tgcgcacaga gcgaatacac tcgtaaacgg gagcaggatt gtgatgcgca
      241 tctccggaac gcaagagttt gctgagatag ctgtcctcga cgaaggtccc ggttttcgaa
      301 ggacgcgggg agaagaagac gggaagcctt tgaaggccgg aggtttgtcc gggatggagt
      361 ggagttcatc ttcaggatgg aattccactg gtcttggtct ccagtacgtt acaatggtcg
      421 ttaggaatct cggaggcaag tttgtcatag aggatctccc cgacggaggg gcgagaacgg
      481 cgatagtcct tgaaaaaaac gcagccggca aagagtcttc gaaggcgggg cgtgaagcct
      541 tcggaatgta aaagatccta atgaaaggat gggaagcgca atgcctcttc acatcggtgc
      601 gatcgacgac gacaggtcta ttctctatac gctcgaagcc atggcctcca cggaagggtg
      661 gcgcatgctc acaacgaacg ttccggagga gtgcctcgac tggatcaaac tcgacgaggt
      721 ggatattctg ctcgtcgact atcatatgcc tgtaatgaac ggtctgcacc tgatacgaaa
      781 ggcgagggag ctctcctcga agctggtgct gatagcgttg accgtggagg acggcggcga
      841 cctcgccggt cagctcgtgc tggccggagc ggacgatttt atcacgaagc cgattcggct
      901 ggcggatttt accgcccgga tcaggttgca cgagaagctc tttgctcacc gggagcagct
      961 gaactgggaa gaacggaaaa aaggcgtcag caaggatacg atgcggaagg tcctcgagaa
     1021 gctgaaggaa ttcagctctc ccaaggactg cgacgatgtt gcggccgctt gcgggctggc
     1081 gtatgtaacg gcgcacaggt atctggaata tcttgccgaa cgcgggttga tcattcgcag
     1141 cgccggggtg caggacggcc gtccgggacg tcccaagaca tactacgcag ttcccgtctt
     1201 atcttcgaac gcggcctctc aataaaaaaa gcggggctct ccgtgcgtgg agagccccgc
     1261 ttcattcagt acgaaaaaaa ctgaagtcta cttaaaccac tgctttttca gagcgtccag
     1321 ctcgccgctt ccctcaagtt cggcaagggc cttgttcaca gcttcgatga gttccgtttc
     1381 gccgagattc atggcaagag ctttccctga accggtgatt tcctgctcga aggctatctt
     1441 gatcttcccc tggaagtcct tgtgctcgac gtagctcttc gcgacgggtg tgtccatcaa
     1501 ggtggcgtcg acacgtccca ggataacttc gcgaacgcaa tcgtcgaact tctggaagga
     1561 ttttaccaca acgtcctcaa gagactggct gtaggtctcc tgtaccgttc caagctggac
     1621 ggctatcgtc ttccccttca tgtccccaag aactttcatc gagtcgtttg ccgcggccac
     1681 gataaacgcg ctgatcgaaa tctcgtaatt cgtggagaag gcaactctct ttgcccgctc
     1741 ttcagtggcg ctcattccgg cggcgacgat atcgatcttt ttcgcgagga gagagggaat
     1801 aaggctgtcg aatggcatgt cgacccattc gatttttttc cctattttcg cggctatcgt
     1861 ctccatcatt tcgatatcga agcctttgag ctgattgttc tcatcccgga actcataagg
     1921 tgggtatgtg ctctccgtac cgacgaggag tacgtccttg gccatgacat tggcccatcc
     1981 ggcagttccc aaaagcaaca gcgcaagcag tgcgaacagg atttttttca tcgttttctc
     2041 tcctccaata tgaacagatg ttaactgctt tcccagtgtg tgataaatat tgtacttgcg
     2101 agcatacacg cctcgttttt tcctgtcaag accgctttcc ccctttttcc aaagactgtt
     2161 ataatttctt ctgacgggag ttctttctcc ggtcgcctga agggtgaaca ggtattcgga
     2221 tcgatggaag gctggg
//
LOCUS       DAOSWW010000425         2233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39035_length_2233_cov_3.885216, whole genome shotgun sequence.
ACCESSION   DAOSWW010000425 DAOSWW010000000
VERSION     DAOSWW010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39035_length_2233_cov_3.885216"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PK442_08715"
     CDS             complement(<1..335)
                     /locus_tag="PK442_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87772.1"
                     /translation="MSRILYSFSVIAFGLLLGYGVRLASERGLVRLRWSMPVLRTFLQ
                     RLALLGLGPFTFIGALWVVEIREPRLALLAGIGAFCHIFGGLAGALLARLFRLERPQA
                     GAMFCCGFFT"
     gene            519..1259
                     /locus_tag="PK442_08720"
     CDS             519..1259
                     /locus_tag="PK442_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOO87773.1"
                     /translation="MDRPVIGIVGNLLIEQAGFSPGMERSYVNNDYVAAVERAGGIPF
                     ILPVVRGQDVAERQTECVDALLISGGYDMDPLLYGEEPIRALEYVYRETDIFQLRTIK
                     TALKRGQPILAICKGIQLLNVACGGTLYQDLGEIPGSFVKHSQNRKRSCPSHTVIAEG
                     ESLIAELFGGAFPVNSYHHQALKEIGRGLVVTSRAMDGVVESVETEDDSFVVGVQWHP
                     EMMLSGGDEMLPLFLRFAEAAERFALSR"
     gene            1256..2005
                     /locus_tag="PK442_08725"
     CDS             1256..2005
                     /locus_tag="PK442_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOO87774.1"
                     /translation="MSIRCDVVLSPSETLPEADCWLVIDILRATTTMCAYFDGGGKRL
                     YPAESIEEAFTLRDRLAREGNAPLLMGERNSLPPPGFDLGNSPLELLAAAPATRPDAV
                     MATTNGTRALLKAASSGAKVYPVCARNANATLRAALGAGERIGILCAGLYGRPAMDDA
                     ACAGLLAEQLLGQGAVLDDGAKMALSVWQAGMRNLSALLGASVHGGRLLSLGFAPDMA
                     YAAEIDASNSVLVLSEVKELPCIVPGNTPAA"
     gene            complement(2027..>2233)
                     /locus_tag="PK442_08730"
     CDS             complement(2027..>2233)
                     /locus_tag="PK442_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87775.1"
                     /translation="DPVKESETASPAPPKETPTVAPPPAEKKRPRTVQPALPPTVRAA
                     PDAMLIETETEKKMRELLQKYNIE"
BASE COUNT          403 a          723 c          724 g          383 t
ORIGIN      
        1 gtgaaaaatc cgcagcagaa catcgctccc gcctgcggtc gttccagccg gaagagacgc
       61 gccagcagcg cgcccgcaag gccgccgaag atgtggcaga aagcgcctat tccggcgagc
      121 agggcaaggc gaggctcgcg tatctccacc acccagagcg ccccgatgaa ggtgaacgga
      181 cccaggccca gaagggcgag ccgctgcaga aaggttcgga gcacgggcat ggaccatcgc
      241 agccggacga gcccccgctc gctcgcgagc cggacgccgt accccagcag cagcccgaag
      301 gcgatgacgg aaaaggaata aagtatccgg ctcatactcc ggcttcgttc tttttttcga
      361 tattatagag cgacttcaca aagaactcct ccttgattcg catgaagggt attattcccc
      421 gtgaagctcc cgatgtcaac gtgaaaaaac gcgcccggcg cgctatactt gaatcacacg
      481 catgcggcat tgaagccgcg caccaggagg ggaaggcaat ggatcgtccg gtcatcggca
      541 ttgtgggaaa tctgctcatt gaacaagcgg ggttttcgcc cggtatggag cggagttatg
      601 tgaacaacga ctatgtggct gcggtggagc gcgcgggagg catcccgttc attctgcccg
      661 tcgttcgcgg acaggacgtg gccgaacgcc agacggagtg cgtcgacgcg ctgctcatct
      721 ccggcgggta cgacatggat ccgctgctgt acggggagga accgatacgc gcccttgagt
      781 acgtctacag ggagaccgac atcttccagc ttcgcaccat caagacggcg ctgaaacggg
      841 ggcagcccat cctcgccatc tgcaagggaa tccagctgct gaacgtcgcc tgcggaggga
      901 cgctctacca ggatctcggc gagatccccg gctccttcgt caaacactcg cagaacagaa
      961 aacgctcctg ccccagccac accgtgatcg ctgaggggga gtcgctcatc gccgaactct
     1021 tcgggggcgc ctttcctgtg aacagctacc accaccaggc gctcaaggag atagggcgcg
     1081 gacttgtcgt cacctcgcgc gcgatggacg gcgtcgtcga gtcggtggag acggaggacg
     1141 actctttcgt cgtcggcgtg cagtggcacc ccgagatgat gctctccggc ggcgacgaaa
     1201 tgctgccgct cttcctccgt ttcgcggagg cggcggaacg cttcgcgctc tcgcgatgag
     1261 catacgctgc gacgtcgtcc tctcgccctc ggagacgctc ccggaggccg actgctggct
     1321 cgttatcgac attctgaggg ccaccacgac gatgtgcgcc tacttcgacg gcggcggcaa
     1381 gcgcctctat ccggcggagt ccatcgagga ggcctttacg ctgcgcgaca ggctggcacg
     1441 ggaggggaac gccccgctcc tgatgggcga gcggaactcg cttcctcccc ccggcttcga
     1501 cctcgggaac tcccctctgg agctgcttgc cgccgcgcct gcaacacgcc ccgatgccgt
     1561 gatggcgacg accaacggca cgagggcgct gctgaaggcc gcgtcaagcg gggcgaaggt
     1621 ctatcctgtg tgcgcgcgga acgcgaacgc gacgctccgt gcggcgctcg gcgccggaga
     1681 gcgtatcggc atcctctgcg cagggctcta cggacgcccg gccatggacg acgcggcctg
     1741 cgcggggttg ctcgccgaac agctcctcgg acagggcgcg gtgctggacg acggcgcgaa
     1801 gatggcgctc tccgtatggc aggccggaat gcgcaacctc tccgcgctgc tgggtgcgtc
     1861 ggtgcacggc ggacgcctgc tctcgctggg gttcgccccg gacatggcct acgccgccga
     1921 gatcgacgcc tccaattccg tgctcgtact gagcgaggtg aaggaactcc cctgcatcgt
     1981 ccccggaaac acccctgccg cttgatgcgt cgaggagtgg cgcgttctac tcgatattgt
     2041 atttctggag cagctcgcgc atcttcttct ccgtctccgt ttcgatgagc atcgcatcgg
     2101 gagccgcgcg aaccgtaggg ggaagcgcgg gctgcacggt ccgcggccgc ttcttttccg
     2161 ccggaggcgg agcgaccgtt ggcgtctcct tcggaggggc cggagacgcc gtttctgact
     2221 ccttgacggg gtc
//
LOCUS       DAOSWW010000426         2231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39080_length_2231_cov_3.695312, whole genome shotgun sequence.
ACCESSION   DAOSWW010000426 DAOSWW010000000
VERSION     DAOSWW010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39080_length_2231_cov_3.695312"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2231
                     /gene="fdhF"
                     /locus_tag="PK442_08735"
     CDS             <1..>2231
                     /gene="fdhF"
                     /locus_tag="PK442_08735"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_except=(pos:732..734,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOO87776.1"
                     /translation="ELGVKDSRFADPGDTNTSHKLEDANPFYVRDLNKCILCGRCVRV
                     CEEHAIYHAIDFQGRGIHTSIQPPVGSPIEESDCVFCGQCVQVCPVGALFEKTAAGQG
                     RPWDLEKVKTVCSYCGVGCELEVNVNKKTGKIANVTTNYSSPTALNKGRSCVKGRFAW
                     QFVHSEDRLTTPLIREGDTFRKASWDEALSLVAEKLGEVKTKHGSDALGFFSSARCTN
                     EENYLMQRLAREVMGTNNVDHCAHLUHSATVTGLGETLGSGAMTNDFESIKSADTILI
                     IGSNTTENHPVIGSFIKERKRKGNCTLIVCDPRKIEMAKYADVYIRQKSGSDVVLLNG
                     LMHVILRENLYASDFIEKNVNNFEKLKETMSKYTPQYVSEITGVSPELIEDAARAYAK
                     GPNSAIFYTMGITQHLNGTDNVRTVANLALLCGMLGRPGTGVNPLRGQNNVQGACDTG
                     CLPEVIPGYQKVNLPATQEKVKELWGSDLPSTPGLSVVEMMHAAGEGKIKGMYIMGEN
                     SMVTEPDSDHVRHALKKLDFLVVQDIFLTETAEFAHVVLPAASWGEKDGTFTNTCRAV
                     QRVRKAVDSPGEARPDWEILLAVGKRLGASWNDMNSPETIFNAIGRIAPAYAGITYAR
                     LEGGALPWPCPTCEHPGTPILYTTGFPRGKADFSPCDWVKPDEWPTEEYPFIATTGRI
                     LYHYHSGSMTRRSSTGEFIKEMYIEVNPADATALNVKDGEQIKVTSRRGEVSGSVKIT
                     DRV"
BASE COUNT          547 a          620 c          608 g          456 t
ORIGIN      
        1 acgaacttgg cgtcaaagat tcacgtttcg cagaccccgg cgatacaaat acttcgcaca
       61 agctggagga tgccaacccg ttttacgttc gcgacctgaa taaatgcatc ctctgcggac
      121 gctgcgtccg tgtgtgcgaa gaacacgcca tctaccacgc catcgatttc caggggcgtg
      181 gaatccacac ctccatccag cctcctgtgg gttcgcccat agaggagtcc gactgcgtct
      241 tctgcgggca gtgcgtccag gtctgccctg taggagccct cttcgaaaaa acagcggcag
      301 gacaggggcg tccgtgggat cttgaaaagg tcaagactgt ctgttcctac tgcggcgttg
      361 gatgcgagct tgaggtcaac gtcaacaaga agacaggcaa aatcgccaat gtcaccacga
      421 attactccag tccgacagct ttgaacaagg gaagatcgtg cgtgaaggga cgcttcgcat
      481 ggcagtttgt ccacagcgag gaccgcctga ccaccccgct tattcgtgaa ggcgacacct
      541 tccggaaagc gtcctgggac gaagcgctct ctcttgtggc tgaaaagctc ggggaagtaa
      601 agacaaaaca cggctcggac gcgcttggat ttttctcctc agcccgttgc accaacgagg
      661 agaactatct tatgcagcgc cttgcaaggg aggtgatggg cactaacaac gtcgatcact
      721 gtgcccatct gtgacacagc gccacagtga cgggtctcgg cgagaccctc ggcagcggcg
      781 ctatgacgaa cgactttgaa tctatcaaat ctgccgatac gattcttatt atcggctcga
      841 acacgactga aaaccaccct gtcatcggct ctttcataaa ggaacgcaaa agaaaaggga
      901 actgcaccct gatcgtgtgc gatccccgaa agatagaaat ggcaaagtac gcagacgttt
      961 atatacgcca gaagagtgga tcggacgttg tacttctgaa tggtttgatg cacgttatac
     1021 ttcgcgagaa cctctacgct tccgatttta ttgaaaagaa cgtgaataac tttgagaagc
     1081 tgaaggaaac catgtccaag tacaccccgc agtacgtttc ggaaattacc ggcgtttccc
     1141 ccgagttgat tgaggatgcc gcacgtgcgt acgcgaaagg gccaaactcc gccatcttct
     1201 atacgatggg cattacgcag catcttaacg gtactgataa cgtccggacc gtcgcgaacc
     1261 ttgcgcttct ctgcgggatg ctgggacgcc ccggaacagg agtcaacccg ttgcgtggac
     1321 agaacaatgt gcagggagcc tgcgataccg gatgccttcc ggaagtgatc cccggatacc
     1381 agaaagtcaa ccttcctgcg acgcaggaga aggtcaagga gctctggggt tctgatctcc
     1441 cttcgactcc gggcctttcc gttgtcgaga tgatgcatgc cgccggcgaa ggaaaaatca
     1501 aaggcatgta catcatgggc gagaactcga tggtcactga acccgactct gaccacgtcc
     1561 ggcatgccct gaaaaaactc gattttctgg tagtgcagga catcttcctt acggagacgg
     1621 cagagttcgc acacgttgtt cttccggccg cgagctgggg cgagaaggat ggaacgttta
     1681 ccaacacttg ccgcgcagta cagagagtcc gcaaagctgt cgactctccc ggcgaggccc
     1741 gccctgactg ggagatcctg cttgcggtag gtaaacgact cggcgcttca tggaacgata
     1801 tgaattcacc ggagacaatt tttaacgcca tcggccgtat agctcccgca tatgctggaa
     1861 taacctatgc ccgccttgaa gggggcgctc ttccgtggcc atgcccgacg tgcgaacacc
     1921 ccgggacgcc gattctgtac acaacgggtt tccccagagg caaggctgac ttctctccgt
     1981 gcgattgggt aaaacctgat gaatggccga cagaagagta tccgttcatt gcaactaccg
     2041 gcaggatcct ctaccactac cactccggaa gcatgacccg ccggagctcg accggcgagt
     2101 ttatcaagga gatgtacatc gaggtgaacc cggccgacgc cactgcgctg aacgtcaagg
     2161 atggagaaca gatcaaggtt acttcccgca gaggagaagt cagcggctca gtgaagatta
     2221 ccgatcgcgt g
//
LOCUS       DAOSWW010000427         2227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39191_length_2227_cov_3.194291, whole genome shotgun sequence.
ACCESSION   DAOSWW010000427 DAOSWW010000000
VERSION     DAOSWW010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39191_length_2227_cov_3.194291"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PK442_08740"
     CDS             complement(<1..816)
                     /locus_tag="PK442_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOO87777.1"
                     /translation="MADTVRVRFAPSPTGALHIGGGHTALFNWLWARHTGGKFILRIE
                     DTDRERSTKEYEDTIMSGMQWLGLDWDEGPDTGGPFGPYRQSERLSTYREAAERLLDE
                     GKAYRDGEAVIFKVPQGVTLAFDDMVYGHIEMQSDTLKDVVMIKSDGMPTYNYAVVID
                     DHTMGITHVIRGEDHISNTPKQLLIYDALGWEYPTFAHLPMILGKDKKKLSKRHGATS
                     VYEYRDMGYMPDSVFNFLALLGWSPGENNEIFSRDEAVGLFDLARVIKKPSVFD"
     assembly_gap    860..869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            959..>2227
                     /locus_tag="PK442_08745"
     CDS             959..>2227
                     /locus_tag="PK442_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOO87778.1"
                     /translation="MSAPDRKKLLLVDGHGLAFRAFYAVRELNAPDGTPTNAIVGFFN
                     MFQKIREDWNPDLCGVVFDAPGPTFRHEAYAEYKAGRKPTPPEFKTQLPIILELLDAM
                     GVPVVSREGVEADDVLASSACAAAVQNLETLILSSDKDILQVLDRDVVVLRPVTGISS
                     FRTMDGKGFEEEYGFSPAAMPDYLALLGDSVDNVPGVPGVGEKTAKKLLSDYGTLEGV
                     YERLDELKGALRKKMEEHREQAFRSRELTRLLCDTEEDLSVFEPRPVEAKRLLEKCRV
                     LGLKSLAAKFQLAESAEEAHEHKKDIRLREVSAEEIAASESVLLLPVIEGKYPLHLSA
                     SELLVLDETGGCRRFPGAEAASLLGRLGELSKGSVLFPDFKEAAACFGSSALSPERVR
                     DFKTAHYLLHPDKNAHLPEAVIPGYESAALA"
BASE COUNT          415 a          658 c          708 g          436 t
ORIGIN      
        1 atcgaacacg gacggcttct tgatgacgcg cgcgaggtcg aagagcccca cggcttcgtc
       61 gcgggagaag atctcgttgt tctcgccggg cgaccagccg agcagcgcga gaaagttgaa
      121 gaccgagtcg ggcatgtacc ccatgtcgcg gtactcgtag acgcttgtgg ccccgtgccg
      181 cttggagagc ttcttcttgt ccttgcccag gatcatcgga aggtgcgcga aggtcgggta
      241 ctcccagccg agcgcgtcgt agatcaagag ctgcttcggc gtgttggaaa tatggtcctc
      301 gccccggatg acgtgcgtga tccccatcgt gtggtcgtcg atgacgacgg cgtagttgta
      361 ggtgggcatg ccgtcgctct tgatcatgac aacgtccttc agcgtgtcgc tctgcatctc
      421 gatatggccg tagaccatgt cgtcgaaggc gagcgtgacg ccctgaggga ccttgaaaat
      481 gacggcctct ccgtcgcggt aggctttccc ctcgtcaaga agccgctccg ccgcctcgcg
      541 gtaggtcgag aggcgttcgg actgtctgta cggcccgaac ggaccgcccg tgtccggtcc
      601 ttcgtcccag tcgagcccga gccactgcat gcccgacatg atggtgtcct cgtactcctt
      661 tgtggagcgc tccctgtccg tatcttcaat gcggaggatg aactttcctc cagtgtgacg
      721 ggcccacagc cagttgaaca gggccgtgtg cccgccgccg atatgaagcg ctcccgttgg
      781 gctcggcgcg aagcgaaccc gtaccgtgtc tgccatttca gttcctcccc gatgacgtcc
      841 ctccgcctgc ggattcgggn nnnnnnnnnt tgtgtgtgca tgaattcctg caataggtta
      901 ttataaccta gttgaacgca gaaaaaacca gttgggtgaa aaaggcaggc ggaacgatat
      961 gagcgcaccg gacagaaaga aactacttct cgtggacgga cacgggcttg ctttcagagc
     1021 tttctacgcc gtccgcgaac tgaacgcgcc ggacggaacg ccgacgaacg cgatcgtcgg
     1081 tttcttcaat atgttccaga agatcaggga agactggaac cccgacctct gcggcgttgt
     1141 cttcgacgcc cccgggccga ccttccgcca cgaggcgtac gcggagtaca aggcggggcg
     1201 aaagccgacg ccgcccgagt tcaaaacgca gctcccgatc attcttgagc tgctcgacgc
     1261 catgggcgtt ccagtggtgt cgcgtgaagg cgtggaggcg gacgacgtgc ttgcttcaag
     1321 cgcgtgcgcc gcggcggtgc agaacctgga gacgctgatc ctctcgtccg acaaggacat
     1381 cctccaggtg ctcgacaggg acgtcgtcgt cctcaggccg gtgactggca tcagttcctt
     1441 tcgtacgatg gacgggaagg gcttcgaaga ggagtacggc ttttcccctg cggcgatgcc
     1501 cgactacctt gcgctcctcg gagattccgt cgacaacgta cccggcgtcc ccggagtggg
     1561 cgagaagacg gcgaagaagc ttctttcgga ctacggaacg ctcgaaggcg tgtacgagcg
     1621 cctggatgaa ctgaagggag cgctcaggaa aaagatggag gagcacaggg agcaggcgtt
     1681 ccggagccgg gagctgacgc gcctcctctg cgataccgag gaggatctct ccgtattcga
     1741 acctcgccct gtggaggcga agcggttatt ggagaagtgc cgcgtcctcg ggctgaagag
     1801 ccttgccgcg aagttccagt tggcggagtc ggcggaagaa gcgcatgaac acaagaagga
     1861 catccggctc cgcgaggtgt cggcggaaga aatagcggct tcggagagtg tgcttctcct
     1921 ccctgtgatc gaggggaagt acccgctgca tctttcggcc tcggaacttc tcgtgctcga
     1981 cgaaacaggg ggctgtcggc gtttcccagg agctgaagcg gcgtcccttc tcggtcgcct
     2041 tggcgaactc tcgaaggggt ctgtgctctt cccggacttc aaagaagccg ccgcctgttt
     2101 cggctcgtcg gcgctctctc cggagcgagt gcgggatttc aagacggcgc actacctgtt
     2161 gcaccccgac aagaacgcgc acctcccgga ggcggtcatc cccggatatg aatcggcggc
     2221 gctcgcg
//
LOCUS       DAOSWW010000428         2225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39239_length_2225_cov_3.064977, whole genome shotgun sequence.
ACCESSION   DAOSWW010000428 DAOSWW010000000
VERSION     DAOSWW010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39239_length_2225_cov_3.064977"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PK442_08750"
     CDS             <1..420
                     /locus_tag="PK442_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO87779.1"
                     /translation="AYYLSFYLSWILPNVKIVSSDMPFEMLANAPKKSLVLGISFPRY
                     STWTVQVLETASELGLVTACLTNDLASPLAVLSEYVLAVPYKPISFIDSFAAPMSVLN
                     CLILSVAQQLGDDVTKKLGILEKRWKEDDVYTQQRGR"
     gene            501..1847
                     /locus_tag="PK442_08755"
     CDS             501..1847
                     /locus_tag="PK442_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOO87780.1"
                     /translation="MDLGHLIPRSFKGNLRTAVRGEGVYIYDEDGNAYMDGCCGALLS
                     SIGHGVKEIADAIHQQLLTMEFAHPSRWNNKVSVEAAEEITSLTQEGLDYVWLVSGGS
                     EAIESAMKIARQYFVERDGDGSGKYVMIARWNSYHGSTVGTMGLAGSMGRRRTFAPLF
                     MEGPKIQAHYCYRCPHNLKYPECNVACARALEGEILRIGPASVAAFFAEPIVGSTVGA
                     LHPPKEYWPIVREICSKYDVLLVADEIMTGMGRSGKPFCTQDWNVTPDIICSAKALAG
                     GYSPVGAMIVKKEIAETIRDGSGAFQHGHTYNGNPVTAAAVTATIRYMKKNNVFENAA
                     AMGARFDEKIPALNEIPIVGEVRGIGMMRGIEIVADRKTKAPFSPSVKAAALVTEECM
                     KRGLVVYPGTGQIGGVAGDQFLFAPPLVATKEEMDMMTDRLEAGLKAAAEALKGKG"
     gene            1890..>2225
                     /locus_tag="PK442_08760"
     CDS             1890..>2225
                     /locus_tag="PK442_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOO87781.1"
                     /translation="MKKGFLALALIGIMVFASTGMADTFITIGSGGVGGTYYPLGGAM
                     AEVLTNAGIGVRATSRSTSASRENCRLVASGRAQIGMTMGSALYQAITGTEAFEPDGK
                     LPLQILMSMY"
BASE COUNT          459 a          639 c          702 g          425 t
ORIGIN      
        1 gcctactacc tttcttttta cctctcatgg attctgccga acgtgaaaat tgtcagctcc
       61 gacatgcctt ttgaaatgtt ggcaaatgcg ccgaaaaaga gtctcgtcct cgggatcagc
      121 tttccgcggt attccacttg gacggtacag gttctcgaaa cagcctccga actggggctt
      181 gtgaccgcct gtctgacgaa cgacctggca agccctcttg cagtgctgtc ggagtatgtc
      241 ctggccgttc cgtataaacc gatttcattc atagactcct tcgcggcgcc gatgagcgtc
      301 ctgaactgcc ttatattaag cgtcgcccag cagttgggcg acgacgtgac gaagaagctc
      361 gggatacttg aaaagcgctg gaaggaggac gatgtgtata cgcagcagag gggccgctga
      421 aacgtgttgt acagcttcgg ttgtctgcgg cgcaggacgc accacaagag ttcgggaaaa
      481 atgaaaggag ttgcttctta gtggatcttg gacatttgat accgcgaagc ttcaagggaa
      541 acctcagaac ggccgtccgg ggagagggcg tctacatcta cgacgaggat ggaaacgcct
      601 acatggacgg gtgctgcggc gcattgctct caagcatagg gcacggcgtg aaggagatag
      661 ccgacgccat ccatcagcag cttcttacga tggagttcgc ccacccgtcg cgctggaaca
      721 acaaggtctc cgtcgaggcg gcggaggaga ttacctccct gacgcaggaa ggtcttgact
      781 atgtctggct cgtcagcggc ggcagcgagg ccatcgagtc ggcgatgaag attgcgcggc
      841 agtatttcgt cgaacgcgac ggcgacggtt ccggcaagta cgtgatgatc gcccgctgga
      901 actcctacca cggaagtacc gtcgggacga tggggctcgc cggcagcatg ggccgccggc
      961 gcacgttcgc accgctcttc atggaggggc cgaagattca ggcgcactac tgctaccgct
     1021 gcccgcacaa cctgaagtac cctgagtgca acgtggcctg cgcccgcgct ctcgaaggcg
     1081 agatcctgcg catcggcccg gcctcggtgg cggccttctt tgccgagccc atcgtggggt
     1141 ccaccgtcgg cgctctgcat ccgccgaagg agtactggcc catcgtccgc gagatctgtt
     1201 ccaagtacga cgtcctgctt gtcgccgacg agatcatgac cggcatggga cgatcgggca
     1261 agcctttctg tacgcaagat tggaacgtga cgccggatat catctgttcc gccaaggcgc
     1321 tcgccggtgg atactcgccg gtgggggcga tgatcgtcaa gaaagagatc gccgaaacga
     1381 tccgagacgg aagcggcgcc ttccagcacg gccacaccta caacggaaat ccggtgaccg
     1441 ccgccgctgt gacggcgacc atccgctaca tgaaaaagaa caacgtcttc gagaacgccg
     1501 ccgcgatggg cgcgcggttt gatgagaaaa tccccgcgct gaacgagatt cccatcgtcg
     1561 gcgaagtgcg gggcatcggg atgatgcgcg gtattgagat cgtcgcggac cggaagacga
     1621 aggcgccgtt ctcgccgtcc gtgaaggcgg ccgccctcgt caccgaggag tgcatgaagc
     1681 gcggtcttgt ggtgtacccg ggaacgggac agatcggcgg cgtggcgggg gatcagttcc
     1741 tcttcgctcc tcccctggtc gcgacgaaag aggaaatgga tatgatgaca gatcgcctcg
     1801 aagcaggttt gaaggctgcc gcggaggcgc tcaaaggaaa ggggtaggaa acgccccttt
     1861 atcttgcacc aaaaaggagg aaattgttca tgaagaaggg atttctggca cttgcgctca
     1921 ttggtatcat ggtgttcgcg tccacaggta tggccgatac gttcatcacc atcggcagcg
     1981 gcggcgtggg cgggacgtac taccccctcg gcggggccat ggccgaggtg ctcaccaacg
     2041 ccggtatagg cgtgcgggcc acatcgcgct ccacttccgc gtcgcgtgag aactgccgcc
     2101 ttgtggcgtc ggggcgtgcg caaatcggca tgaccatggg gtccgcgctc taccaggcga
     2161 tcaccggcac ggaggctttc gagccggacg gcaagctccc cctgcagatt ctcatgagca
     2221 tgtac
//
LOCUS       DAOSWW010000429         2224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39265_length_2224_cov_3.200553, whole genome shotgun sequence.
ACCESSION   DAOSWW010000429 DAOSWW010000000
VERSION     DAOSWW010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39265_length_2224_cov_3.200553"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="PK442_08765"
     CDS             <1..143
                     /locus_tag="PK442_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO87782.1"
                     /translation="TCVREGKQPLVGITDGKRALEVAVSVLKQIHLDDQQFPEGRQAV
                     SC"
     gene            342..560
                     /locus_tag="PK442_08770"
     CDS             342..560
                     /locus_tag="PK442_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliR"
                     /protein_id="HOO87783.1"
                     /translation="MPIINIQALIALALFLASLFIARIVVRIQNGSLPGGALWVLYLR
                     MLLGFLFAGAIVLAFYSFAGIDIISKNL"
     gene            1798..>2224
                     /locus_tag="PK442_08775"
     CDS             1798..>2224
                     /locus_tag="PK442_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87784.1"
                     /translation="MLSLFSPSISREQDKESFILEGTISGPFRTYMSLNSAVVTSVWN
                     ERLLGGAYVPSVSEHHGMTLYFKPDDAARSRNGEVPAASGREVLGALMEAWHSRRGDI
                     ANIVVAASTPFSFEETVQLTGFCLQNRVRIFVFADSLPSV"
BASE COUNT          473 a          523 c          593 g          635 t
ORIGIN      
        1 tgacgtgtgt tcgcgaaggg aaacaacctc ttgtgggcat cactgacggg aaacgggctt
       61 tggaagtcgc ggtttccgtc ctgaaacaga tacacttgga tgatcagcaa ttccccgaag
      121 gccgccaagc tgtttcatgt tgagtatcct ctctgaaatc gtgttatagt ggtaccccgg
      181 aggtaatcga tccggggtac tttttttcga cagggcatgt gtttttttct tttttttctc
      241 ctcgcacgag aggtaaaatc tttatttgaa gtggcgtaat ggttttttat agggtatcct
      301 aatcatgatt accctaattc acacacgaag cggagaatgc catgcctatt atcaatatcc
      361 aagcgctgat cgcccttgct ctttttcttg cttcgctgtt tatagcgaga atcgttgtcc
      421 gtattcaaaa tggttcgttg cctggcgggg ctctgtgggt tctctacctg agaatgcttc
      481 ttggttttct ttttgccggg gcgattgtac ttgcttttta ttcttttgcc gggatagata
      541 taatttcaaa aaatctttga tgggcagtgg cttgggaagg ggacgaaaga gacgatgttg
      601 tttttcaaac gcctgaggag gcatatcatt gtacttcttc tattttccgt ccttttctct
      661 ggagcgccga cggacggcgc cactcttccc ccagccgaca gagaggaatc ggagcgtttt
      721 tttgcgctgg ggtacgaaga ctttattaaa agggagtatg cttctgcgct tgaaaatcta
      781 gacagagcat tgaagctgaa tacgtatctt gttgactact atcttctgcg cggtctcgtc
      841 cttcatcgta tcggacgaac cgacgaggct gtgaagtcga tacgttacta cctggaagtt
      901 cgcccaaggg acagcgctgc ccccagaatt ttggaacgct atcgggatga gcaatttttc
      961 atagaacagt tcctttcagg ggaaccttta caatctgcag ttgtctcctc caggaaagat
     1021 gtcaaaacgg ctttttcgct gggaactctg caaattccag gcgtaaacgg tctggggaaa
     1081 gcaacgtcgt attcagacgg cgttttcctt tcggatacct ttgggaacag agtgggtttt
     1141 cgtctccctg gcgaaaagcc tttccagttt gtcgagatac cctccccggt ggttgcgctt
     1201 ccaacaggag gtaaaagctt ttatgtagta tcagagaacg gggaggtctt ttctcttccg
     1261 gaaggggaac gtagtccggt acggataggg aaaatttcaa cgggcctctc agacgcagcg
     1321 ttagtgacaa acagcctgct tgcggtttcc agcacgacgt ccagagaaat caccctgtac
     1381 tcgcttccgg aactcagacc ttccggacgg ctcgagtttc cggaagggga tcggcttttc
     1441 gaacccgttt ccattgccgt atatggaggg tggattgcag tcgcagacag gaataattcc
     1501 aaggtgtatg ttcagtcctt tcttgatgcg caacgccgct ttgtgataga ggcggatacc
     1561 ccgcgggacc ttgaatggtc ttcattggga gaccttttta ttctccatga gtcgggaaat
     1621 gtgacgaaat gcagcatctc tttttcagga gagcgcgcgc cggcatcgga gacggtactt
     1681 tccgatgcgc gcgaaggctg gagtctcttt tcgatccatg accgggtttt ttgtatcgat
     1741 atttccggct caagactttg ggagctgttt ccggcgcctc gcggagagag cctcgcgatg
     1801 ctctctcttt tctccccttc aatctcgcgg gagcaggaca aagagagttt tattctcgaa
     1861 ggaacaatca gcgggccgtt tcgaacgtat atgtccctga actccgctgt tgtgacttcc
     1921 gtctggaatg agcgccttct cggcggagcg tacgttcctt ctgtttcgga gcatcatgga
     1981 atgacgctgt actttaaacc tgatgatgca gcccgatcac gtaacggcga ggttcccgcg
     2041 gcttccggga gagaagttct gggcgcgttg atggaagcgt ggcattccag aaggggcgac
     2101 atagccaata ttgtcgttgc ggcgagcacc cctttttcgt ttgaagaaac agtccagctc
     2161 acgggttttt gtttgcaaaa ccgtgtgcga atcttcgtat ttgcggacag tctcccgtcc
     2221 gtag
//
LOCUS       DAOSWW010000430         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39434_length_2218_cov_3.123902, whole genome shotgun sequence.
ACCESSION   DAOSWW010000430 DAOSWW010000000
VERSION     DAOSWW010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39434_length_2218_cov_3.123902"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /gene="rplF"
                     /locus_tag="PK442_08780"
     CDS             complement(<1..277)
                     /gene="rplF"
                     /locus_tag="PK442_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOO87785.1"
                     /translation="MSRIGRKPIPVPSGTTVALEGGAVVVKGPKGRLETPMVPGIDVA
                     VEGGNIVVTRQNEQKQTKAFHGLIRALVANMVTGVSAGFKKDLEIVGV"
     gene            complement(294..698)
                     /gene="rpsH"
                     /locus_tag="PK442_08785"
     CDS             complement(294..698)
                     /gene="rpsH"
                     /locus_tag="PK442_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOO87786.1"
                     /translation="MYVTDPIADMLTRVRNANMVYHESVDVPMSKIKMEIARILKEAG
                     YIKNFKVVNDPKKAYSSIRLFLSYGPNRERVIQGLRRISKPGRRVYVGHADLPKVMGG
                     LGIAVISTPKGLKTDTEAGTLGLGGEVLCYVW"
     gene            complement(727..912)
                     /locus_tag="PK442_08790"
     CDS             complement(727..912)
                     /locus_tag="PK442_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOO87787.1"
                     /translation="MARKAIKHKAAQPPKFAVRKYNRCPLCGRVHAFMRRFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            complement(929..1471)
                     /gene="rplE"
                     /locus_tag="PK442_08795"
     CDS             complement(929..1471)
                     /gene="rplE"
                     /locus_tag="PK442_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOO87788.1"
                     /translation="MAPRLLEKYKNDVSPKLAKEFSYKNVMEIPRLKKVVVNIGVGDA
                     KLDIKFMDNAIAELGAITGQRPLLKRAKKSVAGFKVRQGMPVACTVTLRGERMWEFLD
                     RLLSLALPSIKDFQGVSRRGFDGRGNYNLGLKEQLIFPEISYDKVIRSRGMNVSLVTS
                     AKTDEEAQALLRELGMPFTR"
     gene            complement(1486..1812)
                     /gene="rplX"
                     /locus_tag="PK442_08800"
     CDS             complement(1486..1812)
                     /gene="rplX"
                     /locus_tag="PK442_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOO87789.1"
                     /translation="MSKMRLKKGDRVRVISGKDAGKEGKVLRCIRDRDLLVIEGVNVV
                     TKHVKPSQKNPQSGIIKQEAPIYASKVMLVCPSCGAATRVGRGYLENGNKVRMCKACG
                     EIVDQV"
     gene            complement(1829..2197)
                     /gene="rplN"
                     /locus_tag="PK442_08805"
     CDS             complement(1829..2197)
                     /gene="rplN"
                     /locus_tag="PK442_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOO87790.1"
                     /translation="MIQLTTVLNVADNSGAKKLFCIQVLGGSKRKWGTIGDVIVASVR
                     EAIPNSNVAKGTVVKAVIVRTKKEIRRADGSYVRFDDNAAVIIDNNGDPKGTRIFGPV
                     GRELRAKKYMRILSLAPEVV"
BASE COUNT          406 a          672 c          585 g          555 t
ORIGIN      
        1 ccacgccgac gatctcaaga tccttcttga aacctgcgct tacgccggta accatgttgg
       61 cgacaagcgc gcggatcaga ccgtggaacg ctttcgtctg tttctgttcg ttctgtcggg
      121 tgacgacaat atttccgcct tcgacggcga cgtcgatccc cggaaccata ggagtttcga
      181 gccgcccttt cggtcccttc acaacgacgg cgccgccttc gagggcaacg gtcgttccgg
      241 agggaaccgg aattggcttg cgtccgattc gagacatcta ctccacctct tccttaccac
      301 acgtagcaga ggacttcgcc gccgaggccg agcgttccgg cttccgtgtc ggttttgagg
      361 cccttcggtg tggagatgac ggcgatgccg aggccgccca tgaccttggg aaggtcggcg
      421 tgtccaacgt agactcttct gcccggtttg ctgatacggc gcagcccctg gatgaccctc
      481 tcgcggttcg gtccgtagga gaggaacaac ctgattgagc tgtaggcctt cttcgggtcg
      541 ttgactacct tgaaattctt aatatacccg gcttccttca ggatgcgggc aatttccatc
      601 tttatcttgc tcatcggcac gtcgacgctc tcatgataca ccatgtttgc gttcctgacg
      661 cgagtgagca tatccgctat cggatcggta acgtacattc ctagtgaccc ccttcagtcc
      721 actcgactac cagctcgact tgacgacgcc gggaattttc ccttcgcgag caagcttcct
      781 gaaacagcag cggcacatgt tgaatcttct catgaaggca tgaacacggc cgcagagcgg
      841 acatctgtta tactttcgca ccgcgaactt cgggggttgc gccgctttat gcttgattgc
      901 ctttctcgcc attttcgact cccccttcct accgggtgaa cggcattccc agctcgcgga
      961 gaagagcttg ggcttcctca tccgtcttcg ccgaggttac aagcgagacg ttcatgccac
     1021 gggaacggat gaccttgtcg taactgatct cagggaagat aagctgctcc ttgagaccga
     1081 gattgtagtt gcctcttccg tcgaaaccgc ggcgggagac gccctgaaag tccttgatgc
     1141 tgggaagcgc gagcgaaagg agacgatcaa gaaactccca catacgctct ccgcgcagcg
     1201 tgacggtgca cgctacgggc attccctggc ggaccttgaa tccggcgacc gatttcttcg
     1261 ctctcttcaa aagcggacgc tgtcctgtaa tcgcgccaag ctcagcgatc gcgttgtcca
     1321 tgaatttgat gtcgagcttt gcgtcgccca caccgatgtt caccacgact tttttaagcc
     1381 ggggtatttc catcacgttt ttgtaactga actccttcgc cagtttcggc gagacgtcat
     1441 ttttgtattt ttcaagcagc cgaggggcca ttcacgctta cctccttaca cctggtccac
     1501 gatctcgccg caggccttgc acatacgaac cttgttcccg ttctcgagat agccgcgacc
     1561 gacgcgtgtt gcagcgccgc aggaggggca tacgagcatg accttgctgg cgtatatcgg
     1621 ggcttcctgc ttgattatgc ccgactgggg attcttctgg gagggtttca cgtgctttgt
     1681 caccacgttc actccctcga tgaccagcag gtccctgtcg cggatgcagc gaaggacttt
     1741 gccttctttg cctgcatcct ttccggagat cacccgaaca cggtcgcctt ttttcagtct
     1801 catcttgctc attatccgtt atcctccgtc atacgacttc gggcgcgagg gagaggatac
     1861 gcatgtactt tttcgccctc agctccctgc ccacaggccc gaaaattcgg gtgcccttgg
     1921 ggtcgccgtt gttatcgatg atcaccgcag cgttgtcgtc gaatcggaca taggagccgt
     1981 ccgcacggcg tatttccttc tttgtgcgca cgatcaccgc cttgacgaca gttcccttgg
     2041 cgacgttgct atttggaatc gcttcgcgga ccgaggcgac gatgacgtcg cctattgtgc
     2101 cccacttgcg cttgcttccg ccgagtacct ggatgcagaa aagcttcttc gcaccggagt
     2161 tgtcggcgac gttgaggact gtagtcagct gaatcatgac ttacgcctcc ccgccgtt
//
LOCUS       DAOSWW010000431         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39458_length_2217_cov_3.217391, whole genome shotgun sequence.
ACCESSION   DAOSWW010000431 DAOSWW010000000
VERSION     DAOSWW010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39458_length_2217_cov_3.217391"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1374)
                     /locus_tag="PK442_08810"
     CDS             complement(202..1374)
                     /locus_tag="PK442_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO87791.1"
                     /translation="MAMIQVVRFDPELDPGRVLAWRYRNPEDPARSDELGTWAQLVVE
                     ESQEAILFRNGQALDLFGPGRHTLKTANMPLLSKAVNLPFGGESPFKAWVWFVNKVHS
                     LDVKWGTPSPVQLQDPKYGVLLPVRSYGQFGVRIEDSRKFLVKLVGARDQFDREALAH
                     HFRGLLLTRIQDLISTYLVHRGISVVEVNAWLDEISEEIHGKIAGLFGDYGIEVLNFF
                     VNSINVPEDDPAVAELKKTLSERAKMEVLGYNYQQMRSFDTLEKAASNEGGGNLMNAG
                     LGLGVGMGVGGAFGRLAGEMGSNLTTGGGEPVPSVRCTKCGAPAGRGAKFCSECGASL
                     PGRVCPSCGKPLPEGAKFCAECGASLVPRCPSCGKEASPGARFCAECGSAIGRKEE"
     gene            1530..>2217
                     /gene="larB"
                     /locus_tag="PK442_08815"
     CDS             1530..>2217
                     /gene="larB"
                     /locus_tag="PK442_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOO87792.1"
                     /translation="MDEQQLLSLLRKVKEGTLDESDFVRQMKALPYRDLGEVKFDTHR
                     QLRKGFAEIVYCPGKSDEQIESIANAFADTQTNILFSRATPHQFELVQAAIPDAAFHE
                     KARIIAVRKKDFPLCRGLTVITAGSSDIPVAEEAAVTAEYMGCAVERLYDVGVAGLHR
                     LLAHVDVLQNSKAIVAVAGMEGALASVVGGLVACPVVAVPTSTGYGANLGGIAPLLTM
                     LNSCASGVAVM"
BASE COUNT          479 a          717 c          603 g          418 t
ORIGIN      
        1 aaccgaaaag aggatcgccc agaaggaaat ccaaaaacct tccgttcgcg ctcctggagg
       61 tacaagcaga aaaataaccg ccgagagccc cgcgaggatc accgacatta tgacaagcaa
      121 aataaaactt ttactgaacg tcctctctgg tgtaatgaac atagtagcta actactccct
      181 tcttgaaatg ccgaatacct gttactcttc tttccggccg atggcggaac cgcactcggc
      241 gcagaatctg gcgccgggag acgcctcctt cccgcaggaa gggcatctcg ggaccagcga
      301 agcgccgcac tccgcacaaa acttcgcccc ctccggcagc ggtttgccgc atgacggaca
      361 gactcttccg ggaagagaag cgccgcactc cgagcagaac ttcgcccccc ggcctgcagg
      421 agcgccgcac tttgtgcatc gaaccgacgg aacaggttct ccccctccgg tggtcagatt
      481 gctccccatt tcgccggcca gtctgccgaa cgctccgccg acgcccattc ctactccaag
      541 acccagaccg gcgttcatca gattgccgcc cccctcgttc gacgcggctt tttcaagggt
      601 atcgaacgag cgcatctgct ggtaattgta ccccagaacc tccatttttg cccgttcgga
      661 gagcgttttt ttcagctcag ccaccgcagg atcgtcctca gggacgttga tcgagttcac
      721 gaagaagttg agcacttcga ttccgtaatc cccgaacagc cccgcgatct tcccatgaat
      781 ctcctcggag atttcgtcaa gccaggcgtt cacctcaaca acggagatac cgcgatgcac
      841 aagatacgtc gagataagat cctgtatccg cgtgagcagc aacccccgga agtgatgagc
      901 cagcgcctct cggtcgaact gatcacgcgc gcccaccagc tttacgagga acttgcgcga
      961 gtcttcgatg cggacgccga actgtccgta cgacctgacc gggagcagga cgccgtattt
     1021 gggatcctga agctgcacag gagagggcgt gccccacttc acgtcaagtg aatgcacctt
     1081 gttcacgaac cagacccacg ccttgaatgg cgactctccg ccgaagggca gattgaccgc
     1141 cttcgaaagc aacggcatgt tcgccgtctt caacgtatgc cttccgggac cgaaaaggtc
     1201 gagcgcctgc ccgttccgga agaggatcgc ctcctgcgac tcctccacca caagctgggc
     1261 ccacgtcccc agttcatcgc tcctcgccgg atcctccgga ttccggtagc gccacgccaa
     1321 aaccctcccc gggtcgagtt ccgggtcgaa ccggaccact tgaatcatcg ccatcgcacc
     1381 caccgccttc gttcggagtt atccctcggg agcatatcgc tgtatctctt cgaatgatat
     1441 tatgctacca taccgccagt cccgtaaagg agagccggac gaaaatccag cagagcggga
     1501 cgttttcttg aagggaatga ttataagcta tggatgaaca gcagctgctc tctctccttc
     1561 gcaaagtcaa ggaaggaacg ctcgacgagt cggactttgt ccgccagatg aaggcgctgc
     1621 cctaccgcga cctcggcgag gtcaagttcg atacgcaccg ccagctccgc aagggtttcg
     1681 ctgagatcgt ctactgcccg ggcaagagcg acgagcagat cgaatcgatc gcgaacgcct
     1741 tcgccgatac gcagaccaat attcttttct cccgagcgac accgcatcaa ttcgaactgg
     1801 tacaggcggc gatacctgac gcggccttcc atgaaaaggc gcgcatcatc gccgtccgga
     1861 aaaaagattt tcccctgtgc cgcggcctca ccgtgatcac cgccggaagc agcgatatcc
     1921 ccgtggccga ggaggcagcc gtgacggcgg agtatatggg gtgcgccgta gaaaggctct
     1981 acgacgtcgg cgtcgcggga ctgcaccggc tcttggcgca tgtggacgtg ctgcagaact
     2041 ccaaggcgat cgtcgcggtc gccggaatgg agggagcgct cgccagcgtc gtgggagggc
     2101 tcgtcgcctg tccggttgtc gccgtcccca cgagtaccgg gtacggagcc aacctcggcg
     2161 ggatcgcgcc gctgcttacc atgctcaact cgtgcgcctc gggggtggcg gtgatga
//
LOCUS       DAOSWW010000432         2213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39582_length_2213_cov_3.302595, whole genome shotgun sequence.
ACCESSION   DAOSWW010000432 DAOSWW010000000
VERSION     DAOSWW010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39582_length_2213_cov_3.302595"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /gene="secY"
                     /locus_tag="PK442_08820"
     CDS             complement(<1..1033)
                     /gene="secY"
                     /locus_tag="PK442_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOO87793.1"
                     /translation="MLDSFRDAFRLPDLKRRILFTLGVLLIFRLGAHVPTPGIDAEAM
                     SSLFDQGGVLGFLDIFAGGALRRFSVFALGVAPYINASIVMQLLVVIFPTLEKMQKEG
                     EDGRKKIVQWTRLAAVGFALVQAIGMTFWLRGLGIFSGQLFEGAIVVCTVTAGAIAVM
                     WLGEEISDHGIGNGISLLIFAGIVARVPEAIIQSWNMIRLGEMNFLVLLLALVLMVGV
                     IAGCIMLQEGQRRLPVQYAKRVVGNKVYGGQSTFIPLKVNQAGVIPIIFASSVLLFPY
                     SIAKFFSGDIALFFQRIFGPGSFVYTIFYVVLIIFFSYFYTAVVFNPADVANNMKKYG
                     GFILGIRPGK"
     gene            complement(1034..1480)
                     /gene="rplO"
                     /locus_tag="PK442_08825"
     CDS             complement(1034..1480)
                     /gene="rplO"
                     /locus_tag="PK442_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOO87794.1"
                     /translation="MSLHELRPAPGSKTKGKRLGQGLGSGTGKTAGKGNKGQKARSGG
                     GVRPGFEGGQMPMMRRIPKRGFNNARFAKVYQIVNVEDIASRFEAGTTVGFEELHAVR
                     LVQKDRLPIKILANGEIDKALTIRANAFSAGAKAKIEAAGGKVEVV"
     gene            complement(1493..1678)
                     /gene="rpmD"
                     /locus_tag="PK442_08830"
     CDS             complement(1493..1678)
                     /gene="rpmD"
                     /locus_tag="PK442_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOO87795.1"
                     /translation="MAKLRITWKKSGIGKPEVQKRTVRALGLRKLNHTVEHNDTPQIR
                     GMVNKVSHLVECVVVED"
     gene            complement(1691..2203)
                     /gene="rpsE"
                     /locus_tag="PK442_08835"
     CDS             complement(1691..2203)
                     /gene="rpsE"
                     /locus_tag="PK442_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOO87796.1"
                     /translation="MIKKKLDAKGMELTERVVAINRVSKVVKGGKRFKFSVLVVVGDG
                     DRYVGVGMGKAKEISEAVRKGIDKAGKNLLEIKKIGNTIPHPIIGGFGAAEVLLKPAA
                     PGTGVIAGGVVRAIMELGGVKDVVSKVIGRTSNPINVAWATMQAVEEMRTPDEILRLR
                     GKKTLDAVAE"
BASE COUNT          442 a          674 c          591 g          506 t
ORIGIN      
        1 gttttcccgg gcggatgccg aggatgaacc cgccgtactt cttcatgttg ttcgccacat
       61 cggcggggtt gaagacgacc gccgtgtaga agtaggagaa gaagatgatg agcacaacgt
      121 aaaagatcgt gtagacaaag ctgcccggcc cgaagatacg ctggaagaac agggcgatat
      181 ccccggagaa gaacttcgcg atcgaatacg ggaaaagaag cacggacgac gcgaagatga
      241 tggggataac tcccgcctgg ttgaccttca gcgggataaa cgtgctctgt ccgccgtaga
      301 ccttgttgcc gacaacacgc ttcgcatact gcacaggaag gcgtctctgc ccctcctgga
      361 gcatgatgca tccggcgata acgccgacca taagcacaag cgcaagcagc aatacgagga
      421 aattcatctc gccgagccgg atcatgttcc agctctggat gatcgcctca ggcaccctcg
      481 ccactattcc ggcgaagatg agcagtgaga ttccgttgcc gattccgtgg tcggaaattt
      541 cctctccgag ccacatgaca gcaatggctc cggcagtaac cgtacatacg acgatggcgc
      601 cctcaaaaag ctggccgctg aaaattccga ggccgcgaag ccagaaggtc ataccgatgg
      661 cctggacaag cgcgaagcca actgcggcga ggcgcgtcca ctgcacgatc ttctttcttc
      721 catcctcgcc ttccttctgc atcttttcga gggtggggaa gatcacgacg agcagctgca
      781 taacgatgct cgcattgatg tacggcgcga cgccgagggc gaagacgctg aatcttctga
      841 gcgctccgcc cgcgaatatg tcgaggaatc ccaggacgcc gccctggtcg aagaggcttg
      901 acattgcctc cgcatcgatt ccaggggttg gtacatgcgc tccaagccta aagatcagga
      961 gcactcccag ggtaaaaaga attcttcgtt tgagatccgg aagtcgaaat gcatccctga
     1021 aggagtcaag cacttagacc acctcgacct tcccacccgc agcttctatt ttcgccttgg
     1081 ctcctgcgct gaaggcgttg gcgcgaatcg tgagagcttt atctatctct ccgttggcca
     1141 aaatcttaat ggggaggcga tccttctgga caagacgcac tgcatgcagc tcttcgaatc
     1201 ctacggtcgt ccccgcttcg aaacgggagg caatatcctc cacgttcacg atctggtaca
     1261 cctttgcaaa gcgggcgttg ttgaaccctc tcttcgggat gcgccgcatc atgggcatct
     1321 gaccgccctc gaatccgggg cgaacgccgc cgccgcttct ggctttctgg cctttatttc
     1381 ccttgccagc ggttttgccg gtgccgcttc cgagtccttg tccgaggcgc ttgcctttcg
     1441 tcttcgaccc gggagccggg cgcagttcat gcaaactcat ggcgcgttcc tcctagtctt
     1501 caacgacgac gcactcgacc agatggctga ccttgttcac catgccgcga atctggggcg
     1561 tatcgttgtg ttctactgtg tggttgagct ttctcagccc tagcgcgcgg acggtgcgct
     1621 tctgcacttc cggcttgccg atgccgctct ttttccaggt aatccgcaat ttggccattg
     1681 cgtatccctc ctattccgct acggcgtcga gggtcttttt acccctgagc cgcaggattt
     1741 cgtcgggcgt ccgcatctct tcgacagcct gcatggtcgc ccatgcaacg ttgatcgggt
     1801 tggaggtgcg accgatcacc ttgctcacaa cgtccttgac gccgccgagc tccatgattg
     1861 cacggaccac tccgcccgcg ataacgcctg ttccgggcgc cgccggtttg aggagtacct
     1921 cggctgcgcc gaagcctccg atgatgggat gaggaatggt gtttcctatc ttcttgatct
     1981 caagaagatt cttccccgcc ttgtcgattc ctttgcggac ggcctccgaa atttccttgg
     2041 ccttccccat gccgacgcct acatagcggt ctccgtcgcc aacgacgacg aggacgctga
     2101 acttgaacct ttttccgcct ttgacgactt tgctcacgcg gtttattgct actacgcgtt
     2161 cagtgagctc catccccttg gcatccaact tttttttaat catcgcatgc gcc
//
LOCUS       DAOSWW010000433         2212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39611_length_2212_cov_3.354196, whole genome shotgun sequence.
ACCESSION   DAOSWW010000433 DAOSWW010000000
VERSION     DAOSWW010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39611_length_2212_cov_3.354196"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PK442_08840"
     CDS             complement(<1..540)
                     /locus_tag="PK442_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87797.1"
                     /translation="MTAEYVGTTLLLEKNLPPDRSLRRRLERCAGFVVEVSSAEEASR
                     LLSEGGVALFVADALFASPRETAEILRSLLAVRPVPLLLVFPSSGEEIADELAVFAQS
                     GFVTQETPESLLRVAIHNSRRLFEIRMCPPVKRTIGQCAACGLRERCELRMSEERFRS
                     LVANIPGVVFRCRSDPEWTM"
     gene            1190..>2212
                     /gene="aroF"
                     /locus_tag="PK442_08845"
     CDS             1190..>2212
                     /gene="aroF"
                     /locus_tag="PK442_08845"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOO87798.1"
                     /translation="MMVVEMAEGSTRRDLDRVCESMAGGGRSYRLVSGRGSAYVMSAD
                     AARGEESAPFRAFPGVRSVNVTTCCHPLASREIGGESRPMELAKGLWIGDGEPLVMAG
                     PCAVESRKQLLRTAEGVQRAGARVLRGGAFKPRSHPYAFQGLGSEGIALLKEARAATG
                     LPVVTEVMSSEEAEWVAPHIDILQVGARNMQNFSLLRTLGGLDRPVLLKRGMCATIEE
                     WLQAAEYILAGGNERVILCERGIRSFDKNTRNTLDLSVVPLLKSMTHLPVVVDPSHAT
                     GRRELVAPMSLAALAAGADGLVVEVHCDPAEALCDGPQSLTPDDFALLMTRASKMLRA
                     LNEEEPT"
BASE COUNT          486 a          634 c          693 g          399 t
ORIGIN      
        1 catcgtccat tccggatcgg agcggcagcg gaagacgacg cccggtatgt tcgccacaag
       61 agacctgaag cgttcctcgc tcatacgcag ctcgcatcgt tcacgcagcc cgcaggcggc
      121 gcactggccg atcgtccgtt tgacaggggg gcacatccgg atttcaaaga gccggcggga
      181 gttgtggatt gcaacgcgaa gaagcgactc gggcgtctct tgcgttacaa aacccgactg
      241 tgcgaagacg gcgagttcgt ctgcgatttc ctcgccggac gagggaaaaa caaggagcag
      301 cggaaccggg cggacggcga gaagcgaacg cagaatttcc gctgtttccc ggggagacgc
      361 aaagagcgcg tcggcaacga acagcgcaac acccccctcc gagagcagcc tgctggcttc
      421 ctcggcggag gagacttcga caacgaaacc ggcgcaccgt tcaaggcgac gcctcagcga
      481 tctgtccggc ggaagatttt tttccagaag gagcgttgta ccgacgtatt cagcggtcat
      541 tctgtaacct cgcaccattc aaatgaatat actgcacttt gaacactctc ttatacttat
      601 ttatcttgga ggagagagaa ggatgaatta cttcaagaca agggattttt tggcgcttta
      661 ctccttgaaa tagcataaat tgtcggagca cactgtatcc ctcttttgcg ttccaaaggg
      721 tgaagtcacg tttaaaaaag tcaacctata aaagtgtagc accttgctct acgcgagtca
      781 aatttactag ctacaaattc tcttaaatta cgtcaataga aaaatagaga gcgcctcttg
      841 acaaatcgtc aagcacccct acaatagttc atcaataccg ctctgcggaa aaatcactga
      901 agaaaggaga cggtcgccgt gacgaaaagc gcagtacgca tgagagaacc cctgacggac
      961 ggagtgttcg cgttcgcgtt tacccgcggc ctgtggcttt attacgccac cggccagtct
     1021 tcgcgtagag atccgtcttc ggcttcttca tatccggcct ctccgggagc agcgcaccga
     1081 taggacgagg cggataccaa gaaaagacaa acgccggaac cgggtttcta cacccggttc
     1141 cggttttttt atttcgtggc tgaatgaatg tgaaaagggg gaaggcatca tgatggtggt
     1201 cgaaatggcg gagggcagca cgcgacggga tctggacagg gtatgcgaat caatggcggg
     1261 cggcgggcgg agttacaggc tcgtctccgg aagagggagc gcctacgtca tgtcggccga
     1321 cgccgcgcgc ggggaggaat ccgcgccttt ccgggcgttc cccggcgtgc ggagcgtgaa
     1381 tgtaacaacg tgctgccacc ctctcgccag cagggagatc ggcggggaat cccgtccgat
     1441 ggagctcgcg aaaggtctct ggatcggcga cggggagccg ctcgtcatgg cgggcccctg
     1501 cgctgtggag agcagaaaac agctgctgag gacggctgaa ggggtacaaa gagcgggagc
     1561 gcgcgtcctg aggggcggcg cgttcaagcc ccgctcgcac ccctacgcct tccaggggct
     1621 ggggagcgag ggaatcgcgc tgctgaagga ggcgcgtgca gccacagggc tgcctgtggt
     1681 cacagaggtc atgtcctccg aggaggcgga gtgggtcgcg ccgcacatcg atattctcca
     1741 ggtgggcgcc cggaacatgc agaacttctc cctgctgagg acgctcggcg ggctggacag
     1801 gccggtgctg ctgaaacgcg ggatgtgcgc caccatcgag gagtggcttc aggcggcgga
     1861 gtatatcctc gccggaggga acgagcgcgt gatcctctgc gaacgcggaa tccgcagctt
     1921 cgacaagaac acccgcaaca ccctcgacct gagcgtcgtc ccgctgctca aatccatgac
     1981 gcacctcccc gtcgtcgtgg accccagcca cgccaccggc agacgggagc tcgtcgcgcc
     2041 gatgagcctc gcggcgctcg ccgccggagc ggacggcctc gtcgtcgagg tgcactgcga
     2101 ccctgcggag gcattgtgcg acgggccgca gtcgctgacc ccggacgact tcgcgcttct
     2161 gatgacgcgg gcgtcgaaaa tgctccgcgc gctcaacgag gaggagccga ca
//
LOCUS       DAOSWW010000434         2211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39634_length_2211_cov_3.585343, whole genome shotgun sequence.
ACCESSION   DAOSWW010000434 DAOSWW010000000
VERSION     DAOSWW010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39634_length_2211_cov_3.585343"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PK442_08850"
     CDS             <1..841
                     /locus_tag="PK442_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO87799.1"
                     /translation="LAIATLAFGFIVEEGLARAAETGDVALQADCLQHLCYYAIQRED
                     GELLCSAASRLSEAAERCGDASLGAMARRFSGMSLLFLGKFEEAERLLQESVAQFRRM
                     EYLDSPYTLQVLAGNCYLGEIRYRTGRVREALSVFCRCVVECEEHGLYRGLCLFCSNA
                     ALAAFDLGEDETAKEYLRKARHYFEGSELRRANAVGLALLALFALRQGKDDEAISFWE
                     SAGNLCVSLRKKSWVAACCRIRDVLERELEAGHLSGQRKNTTTLNAFSANCRTFKEVC
                     GDD"
     gene            complement(850..1017)
                     /locus_tag="PK442_08855"
     CDS             complement(850..1017)
                     /locus_tag="PK442_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87800.1"
                     /translation="MEIHLHTEEIEELKSLMEEDEFTGAVEVFAREMLLEGMSYHKAR
                     QPGGYLHPEEK"
     gene            complement(1213..>2211)
                     /locus_tag="PK442_08860"
     CDS             complement(1213..>2211)
                     /locus_tag="PK442_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HOO87801.1"
                     /translation="VVDGICLLGIAGGVAAVLGVGTMQISSGIQILTGLTADKTMWIA
                     VVSAMAAVYVISSYTGLNRGIRWLSDKNAKIFLALMIFVFVFGPTRFILSLGTQATGH
                     FINEFFIRTHYLSAVDGSAWPRWWPIYYWAIWLAYAPLIGMFLARLAKGRTIRQFLLM
                     NLMVPAIFGLLWFSVFGGAAIHLELTGQGLWAAIHEGGKLMLEKSVFEFLKHYPLANI
                     LSWVFVGTVFISIVTMADSMTSTVASLSTTAAHQEGKEPPATLKIFWGAVMSSVAIIN
                     LISGSGGGEISGIDATKQISTAAGFPILFLMMLMAFTICWMIIRHSRGEKVLLVKE"
BASE COUNT          470 a          602 c          653 g          486 t
ORIGIN      
        1 tctggcgatc gccacgctcg ccttcggctt tatcgtcgag gaggggcttg cccgcgctgc
       61 agaaacgggt gacgtcgcct tgcaggccga ctgccttcag catctttgct actacgctat
      121 ccaaagagag gacggagagc tgctgtgttc cgcagcttcc aggctgagcg aagcagcgga
      181 acgctgcggc gatgcgtcgc ttggcgccat ggcgcgcagg ttttccggga tgtcgctgct
      241 ctttctggga aagttcgagg aggcggagcg cttgttgcag gagtccgtcg ctcaattccg
      301 gcggatggag tatctcgact ctccgtacac gctccaggtg cttgcaggaa actgctacct
      361 cggagagata cgctatcgta ccggacgcgt tcgcgaagct ctttccgttt tttgccggtg
      421 cgtcgttgaa tgcgaggaac acggcctgta ccgtgggctt tgcctctttt gcagcaacgc
      481 ggcgctcgcc gcgttcgatc ttggggaaga cgagacggcg aaggagtatc tccgcaaggc
      541 gaggcactac ttcgagggca gcgagctgcg gcgggcgaac gctgtcggtt tagcgcttct
      601 tgcgctcttc gccctccgcc aaggaaaaga tgacgaggcg atctccttct gggaatcggc
      661 cgggaatctg tgcgtctcgt tacgaaaaaa gtcctgggtc gctgcgtgtt gccggatacg
      721 tgacgtgctc gagcgggaac tcgaagcagg gcatttgtca gggcagcgaa aaaacacaac
      781 aacattaaac gctttttcag cgaattgcag aacgttcaaa gaggtgtgcg gcgacgatta
      841 aaggagtcct tacttttctt ccggatgcag ataccctccg ggttgtcggg ccttatgata
      901 ggacatgccc tccaaaagca tctccctcgc gaaaacctcc acagctcctg tgaattcatc
      961 ttcctccatc agacttttca attcttctat ctcttctgtg tgcagatgga tttccatagc
     1021 cagatcgtcc ctccttgttt tccgggtagt actaataatc ttagtatatt tgaaatatta
     1081 cgtcaaggga agatgcagga aatcgctcat ctgaatgatt cgtctgtgtg gaagaaacga
     1141 aaaacgtccg atgggcatga aaaaacccgg gagctgcgcc cccgggcctt ctttttcaaa
     1201 tcgctctttc ggttactcct tcacgaggag cactttctca ccccttgaat gacggatgat
     1261 catccagcag atggtgaacg ccatcagcat catcaggaag aggatcggga atcccgccgc
     1321 agtggaaatc tgtttggtcg cgtcgatgcc gctgatctcg ccgccgccgc tgccgctgat
     1381 caggttgatg atcgccacgg aggacatcac cgctccccag aagatcttga gggtcgcggg
     1441 gggttccttt ccctcctggt gggcggccgt cgtggagagg cttgctaccg tcgaggtcat
     1501 ggagtcggcc atggtgacga tcgagatgaa gaccgtgccg acgaagaccc agctcagtat
     1561 attggcgagc ggatagtgtt ttaaaaattc gaagaccgat ttctcgagca tcagcttgcc
     1621 gccttcgtgt atggccgccc acagtccctg tcccgtcagc tcaaggtgaa tcgccgcgcc
     1681 tccgaaaacg gagaaccaca gcaggccgaa gattgcggga accataaggt tcatcagcaa
     1741 gaactgccgg atggtgcggc ctttagcgag ccgtgcgagg aacataccaa tgagcggcgc
     1801 gtaggcgagc cagatggccc agtagtagat cggccaccat cggggccagg cggaaccgtc
     1861 gactgcgctc aggtagtggg tccggatgaa gaattcgttg atgaaatggc ctgtggcctg
     1921 cgtgccgagt gaaaggatga agcgcgtcgg cccgaagacg aagacgaaga tcatcagggc
     1981 aaggaatatc tttgcgttct tgtcggagag ccagcggatt ccgcggttca atccggtata
     2041 actggagatg acgtagactg cagccatagc tgaaacgacc gcgatccaca tcgtcttgtc
     2101 ggcggtgagt cctgtaagta tctggatgcc gctgctgatc tgcatcgtac ccactccgag
     2161 gaccgccgct acaccgcccg ctatgccgag caggcagatg ccgtcgacaa c
//
LOCUS       DAOSWW010000435         2209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39706_length_2209_cov_2.315692, whole genome shotgun sequence.
ACCESSION   DAOSWW010000435 DAOSWW010000000
VERSION     DAOSWW010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39706_length_2209_cov_2.315692"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK442_08865"
     CDS             complement(<1..268)
                     /locus_tag="PK442_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87802.1"
                     /translation="MTESLRFHHYYVLASKGIQEYILRGDKLKLMIGGSELIDKLSSN
                     LVERLLHAMGLSPESDFTILMKAAGGVRILFAEKEYAERFAALLP"
     gene            complement(488..1369)
                     /locus_tag="PK442_08870"
     CDS             complement(488..1369)
                     /locus_tag="PK442_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87803.1"
                     /translation="MIVIVGIVAFIYAKSDTSSSRPAGRPAERYGRPETTPAGRTNSR
                     TPEQAHPATRRVSSTDGNTAAAYSPAAYTAPAATTTRTVSQTRGPEGGTQGRLTEHAV
                     INGEGEVYLVDTALSGSPFYGPVWDRILTALEREEYVRARILFRRRAANNYFSGYETD
                     IDGVHGFLPRSKCGYFWNEELDATGKSIIVKPFLVHPSGPRKGSLLVDAVAPLHELDK
                     EKQREWSIGIDFDPQKFYLACGKYKTLWCHPSEIASILHPSLGIRSLSEATGRYYRIA
                     SSRTGEPGRVLPIEVLS"
     gene            complement(1366..2088)
                     /locus_tag="PK442_08875"
     CDS             complement(1366..2088)
                     /locus_tag="PK442_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87804.1"
                     /translation="MDQIFCDASYKNGVCGVAAATPLTLWNKDKETRKKSVFHKISHL
                     FQSAILGKEGYVVFFSTFPCAGSNEAEIKALGLAIAMAEDILLSESRNGRPVEISSDS
                     LVALGDVLASEEPLFKESEVLRNRVATRKIILSKVKAHAGHQGNEIADEWSKKARRGI
                     ESRMEKQPDTPPAEQTPRSHEDGGKEESICGFTERLLKETVSAAVITSDGRIVPRSLA
                     FKSGDRNYTELFKERVWGGCWD"
BASE COUNT          405 a          593 c          617 g          594 t
ORIGIN      
        1 ggggaagaag agcggcgaaa cgctccgcat actccttttc ggcgaagaga atgcgcactc
       61 cgccggctgc cttcatcaga attgtgaagt cgctttccgg ggaaagtccc attgcgtgca
      121 gcaatctttc gacgaggttg gaggagagtt tgtcgatcag ttcgctcccc ccgatcatga
      181 gtttcagctt gtctccgcgg agtatgtact cctggatgcc tttggacgca agtacgtagt
      241 agtggtggaa tcttagggat tcagtcacag gcaactcctt cctttcggat atggatgggt
      301 gctcctatgt aatgagaggt tgctctcggt cagcgctact ccccccaatt tttggttgtt
      361 gttacgggat tggagcttta gggtcccctg cttttttctc ggcttagaaa cgttttttat
      421 tttacatcga aaagaagctt ttgcggagat tctctttgtg ccttttgaat catttgatgc
      481 tttttagtta tgaaagcact tcgatgggaa gaacacggcc aggttctccg gtacgcgagg
      541 acgctattct gtagtagcgt cccgtggctt cggagagcga gcggataccg agggaagggt
      601 ggagaatgga cgctatttcc gacgggtgac accagagcgt tttatactta ccgcatgcga
      661 ggtagaattt ttgagggtcg aagtcgatac ctatgctcca ctcccgctgt ttttccttgt
      721 caagttcatg gagcggagcg accgcgtcga cgagcagact tccctttctt ggtcccgagg
      781 gatgtacaag aaaaggtttg acgatgatgc tttttcccgt ggcgtcgagt tcttcattcc
      841 agaagtagcc gcatttactt ctcgggagga atccgtgaac gccgtcgata tcggtttcgt
      901 acccggagaa gtagttgttg gcggctcttc tgcggaagag aatccgcgca cggacatact
      961 cttcgcgttc aagcgccgtg agtatcctgt cccatacggg accgtagaac ggggagcccg
     1021 aaagcgcggt atcgacgaga tagacctcac cttctccgtt gatgacggcg tgttccgtta
     1081 accttccctg cgtcccccct tccgggccgc gggtctgcga aacggttcgc gttgtagtgg
     1141 ctgcgggagc ggtatacgca gccgggctgt aggctgccgc cgtgtttcca tctgtggaag
     1201 atacgcgacg tgttgcggga tgcgcttgct ccggagttct tgaatttgtc cggccggcgg
     1261 gagtcgtctc cgggcgtccg tatctttcgg cgggcctgcc cgccggtctc gacgaagagg
     1321 tatccgattt cgcgtatatg aaggcgacaa tgcctacaat gactatcaat cccagcaacc
     1381 gccccacacc ctttctttga agagttccgt atagtttcgg tcgcctgact tgaacgcgag
     1441 gcttctaggg acgatgcgtc cgtcgcttgt tatcactgcc gcagaaactg tttctttcag
     1501 caggcgctcc gtaaagccgc aaatagattc ctccttgccc ccgtcctcgt gcgagcgggg
     1561 agtttgctct gcaggagggg tatccggctg tttttccatc ctggactcta ttcctctccg
     1621 ggcttttttg gaccattcgt cggcgatctc gttcccctga tgtccggcgt gcgcctttac
     1681 cttgctcaag atgatctttc tggtggcgac ccggtttctg agtacttcgc tctctttaaa
     1741 aagaggctct tcggatgcaa gtacgtctcc gagcgccaca aggctgtcgc tgcttatctc
     1801 gacgggacgc ccgttcctgc tttccgagag gagtatgtcc tccgccatgg cgatggcgag
     1861 gccgagagcc ttgatctcgg cctcgttgct tccagcgcag gggaaagtgg agaaaaagac
     1921 gacataccct tcttttccca atatagctga ttgaaagaga tgagatatct tgtggaagac
     1981 gctctttttc cgggtttctt tgtctttgtt ccagagcgtg aggggagttg ccgccgcaac
     2041 gccgcacacg ccgtttttat acgaggcatc gcagaatatc tggtccattt taccgtccgg
     2101 gccattcctt cgtacgcttg tacacttccc acgcgttaag ccatgcggcg gtccagccca
     2161 cgacggtggc tgccgtggtc tcctttccga atccttctcc gcctcctcc
//
LOCUS       DAOSWW010000436         2208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39732_length_2208_cov_3.149559, whole genome shotgun sequence.
ACCESSION   DAOSWW010000436 DAOSWW010000000
VERSION     DAOSWW010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39732_length_2208_cov_3.149559"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PK442_08880"
     CDS             <1..733
                     /locus_tag="PK442_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO87805.1"
                     /translation="FVIGLLNNVIPFSLIIQGQMHIASGLASILNATTPLFTVVVAHF
                     LTDDEKMTAGRFLGVLVGFSGVVLMIGPEAVAGLGTHVVAQLCVLGATLCYAFAGVFG
                     RRFRGMKLAPMIAAAGQVTASSIILVPLALVLEHPWTLPAPAPEVWGAILGLALLSTA
                     FAYVIYFRILASAGANNVLLVTFLIPVSAILLGTTVLGERLEPKHFAGMALIGVALAA
                     IDGRLFGMLRRFFRTSPGDNPGDCE"
     gene            749..>2208
                     /locus_tag="PK442_08885"
     CDS             749..>2208
                     /locus_tag="PK442_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87806.1"
                     /translation="MFRRKMKGSVLVALFLLAALLPQAPRLEAAPPGTTDPFARAPFL
                     LMDEEAVVLTQESGNIYSDVFLPNVVQREDGSRAWSERLDDAKTTWSPRPPLLDGKVG
                     DAAVLLRNSRNEAFLAQVWNAISSTGLTMSLAPVDQNGYKAEIMRINSRWGFFSINYF
                     VAAAGDLNGDRATELVLVSTGDSNGPIFFTASVAGFDNPVGSEFSNAGEVPRIHWRSG
                     FRIATGDADGNGRDEIAVMYVSEAGKYVVALFKADENLRLRLLGVHTLDIAPYRDTYY
                     PPDQFDIAFADWEGDGRKKIFCVTGYAGDGFGRNVRLLTVTIQEGRATFTTESRHSHK
                     FFGDQGPILAAAADINGNGKDELLLTNRWGSPPFERSELIAVYNKGDGSSGTASKYWS
                     NDRLEIYRPISHLKTGRFTGSLHPSAAAKGGSHAIVLLHHTSLSSGGTFARLLRLEGD
                     TLKEVSAARLSGFSNPDRAHLLVGDLDRDSMVLGYPT"
BASE COUNT          401 a          703 c          677 g          427 t
ORIGIN      
        1 cttcgtgata gggctactga acaacgtgat ccccttcagc ctgatcatcc aggggcagat
       61 gcacatcgcc agcggtcttg cctcgatcct caacgcgacc acgccgctct tcaccgtcgt
      121 ggtcgcgcac tttttgaccg acgacgagaa gatgaccgcc gggcgttttc tcggcgtgct
      181 cgtcggtttc tccggcgtcg tactgatgat cgggccggag gccgtcgccg ggctgggaac
      241 gcacgtcgtc gcgcagctct gcgtcctggg ggcgacgctc tgctacgcct tcgcgggcgt
      301 gttcggcagg cgcttccggg ggatgaagct cgccccgatg atcgctgccg ccgggcaggt
      361 aacggcatcc agcatcatcc tcgtaccgct cgccctcgtc cttgaacatc cgtggacgct
      421 tccagcgccg gcgcccgagg tgtggggcgc gattctcggg cttgcgctgc tctccactgc
      481 gttcgcctac gtcatctact tccgcatact ggcctcggcg ggagccaaca acgtgctcct
      541 tgtgaccttt ctcatcccgg tcagcgccat actcctcgga accacagttc tgggtgaacg
      601 cctggaaccg aagcacttcg ccggaatggc gctcatcggc gtcgccctcg ccgcgatcga
      661 cggcaggctc ttcggcatgt tgcggcgctt tttccgtact tccccgggag acaacccggg
      721 agattgcgag tgaaaaggag gtaccgacat gttccgacga aaaatgaaag gttctgtgct
      781 tgttgcgttg tttctgctcg cagcgctgct tccgcaggcc ccccgcttgg aggcagctcc
      841 gccgggaacg acggacccgt tcgcccgcgc gcccttcctg ctgatggacg aggaggcggt
      901 cgttctgacg caggagagcg gcaacatcta cagcgacgtc ttcctgccga acgtggtgca
      961 gcgcgaggac ggctcccgcg cctggtccga acgactggac gacgccaaga cgacctggtc
     1021 gccgagaccg ccgcttctgg acggcaaggt cggcgacgcg gccgtgctgc tgcggaacag
     1081 ccggaacgag gcgtttctgg cgcaggtctg gaatgcaatc tcttcgacgg ggttgacgat
     1141 gtcgttggcg cctgtggacc agaatggcta caaggcggag attatgcgaa taaatagtag
     1201 atggggtttt ttttcaatca attatttcgt cgccgccgcg ggggatctta acggggatcg
     1261 ggccacggaa cttgtcttgg tcagtaccgg cgatagtaac ggaccgatat ttttcacggc
     1321 ctccgtcgcc ggattcgaca atcccgttgg aagcgagttt tcgaacgcag gcgaagtacc
     1381 tcgcatacac tggcgctccg gattccgcat cgcgaccggc gacgccgacg ggaacggcag
     1441 ggacgagatc gccgtgatgt acgtatcgga ggcgggaaag tacgtggtgg cgctcttcaa
     1501 ggcggatgag aacctgaggc tccgtctcct cggagtgcat acgctcgaca tcgcgcccta
     1561 ccgcgacaca tactaccccc cggaccagtt cgacatcgcc ttcgccgact gggaaggcga
     1621 cggaaggaag aagatcttct gcgttacagg gtacgccgga gacgggttcg gaagaaacgt
     1681 ccgcctgctc accgtgacca tccaggaagg aagagcgacc ttcaccacgg aatcgagaca
     1741 ctcccacaag ttcttcgggg accagggccc catactggcg gccgcggcgg acatcaacgg
     1801 aaacggaaag gacgagcttc ttctgaccaa tcgatgggga agccccccct tcgagcgttc
     1861 ggaactcatc gcggtgtaca acaagggcga cggctcctcc ggaaccgcca gtaagtattg
     1921 gagcaacgat cgactcgaga tctaccggcc catctcccat ctgaagacgg ggcgcttcac
     1981 cggctcgctc cacccgtcgg cggcggcgaa aggcggatcg cacgcgattg tcctgctcca
     2041 tcacaccagt ttgtcctccg gcgggacgtt cgcccgtctt cttcgtcttg agggcgatac
     2101 gttgaaagaa gtttccgcag ctcgtctcag cggcttctcg aatcccgacc gcgcccatct
     2161 cctcgtcggc gacctcgacc gcgacagcat ggtgctgggc taccccac
//
LOCUS       DAOSWW010000437         2206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39805_length_2206_cov_2.987913, whole genome shotgun sequence.
ACCESSION   DAOSWW010000437 DAOSWW010000000
VERSION     DAOSWW010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39805_length_2206_cov_2.987913"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PK442_08890"
     CDS             complement(<1..560)
                     /locus_tag="PK442_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOO87807.1"
                     /translation="MSLCSLSAVEIARGIRNRDFSALEILRSFYQRAETLEPSLHALL
                     HCAPEIAEKHAADVDAAVARGEDPGPLAGVPVVLKDNMCVRGMPATCASRILQGWIPP
                     YDATVTELLETSGAVVAAKGNMDEFAMGSSTEFSAFGPVSNPWDTERVPGGSSGGSAV
                     SVAAGYTPLALGSDTGGSIRQPASFCG"
     gene            complement(557..913)
                     /gene="gatC"
                     /locus_tag="PK442_08895"
     CDS             complement(557..913)
                     /gene="gatC"
                     /locus_tag="PK442_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOO87808.1"
                     /translation="MTPRDSSACALDTKAVRHVADLARLRVEDEEMDGLVRHFGAILE
                     HFNVLSAAGKDGRLDLDAVDPFLFSDTEIPPAREDVPIVSSVRGDVLGSAPRRKDSFF
                     VVPRILAQHDGEEFSS"
     gene            complement(1130..>2206)
                     /gene="ligA"
                     /locus_tag="PK442_08900"
     CDS             complement(1130..>2206)
                     /gene="ligA"
                     /locus_tag="PK442_08900"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOO87809.1"
                     /translation="TRLEDIVVSVGRTGALTPVAVLSPVTLLGSVVRRASLHNEDELR
                     RKDVRIGDMVRVRKAGEIIPEILGPVLDARTGEEREFIMPERCPACGTPAVRLEGEVA
                     LRCPNRSSCPAQLKEGLRHFASRSGMDIRGLGDKIVDQLVEKGLLRSLADLYTLDEET
                     LASLDRMGEKSARNLINALKQSKDRPFSRLLSALGIRFTGSRSAEILAEVFRDLPTLR
                     DASEETLAAVDGVGPVMASAIRSFFEQPENQRLLERLEAAGVRATLPRLEDERENEPI
                     GSRPFAGMRLVFTGEMESMTRSEAEKMAKRRGAVCSSSVSKKTSFVVAGKDAGSKLSK
                     ALSLGVKVVDEEEFLEMTRLTDEA"
BASE COUNT          478 a          682 c          615 g          431 t
ORIGIN      
        1 ccgcagaagg aggccggttg gcggatcgaa ccgccggtat cgctcccgag ggcaagcggg
       61 gtgtatccgg cggcgacgga gacggcgctg ccgccggagc tcccccccgg aacgcgctcc
      121 gtatcccaag ggttcgagac cggtccgaag gccgagaact ccgttgaact ccccatggcg
      181 aattcatcca tattgccctt tgcggcaaca acagccccgg atgtctcaag cagctcggtc
      241 accgtagcgt cgtacggagg aatccacccc tgcaaaatcc tgcttgcgca agtcgcgggc
      301 attccgcgga cgcacatgtt gtccttgagc acaacgggca ctccggcaag aggaccgggg
      361 tcttcccctc gcgcgacggc agcgtcgaca tcggcggcat gcttttcggc aatctccggg
      421 gcacaatgaa gaagtgcatg caaagagggt tcgagggttt ctgcacgctg ataaaaggac
      481 ctgagaatct ccagggcgga aaagtcgcga ttccgtattc ccctggcgat ctcgactgcg
      541 gagagcgaac agagactcat gatgagaact cctctccgtc gtgctgcgcg agaattctcg
      601 gcacgacaaa aaaagagtcc tttcttcgag gcgccgagcc aagaacatcc ccgcgcacac
      661 tcgaaacgat tgggacatct tcccgcgcag gaggaatctc cgtatccgag aatagaaacg
      721 ggtcgacggc gtcgagatcg agacgtccat ctttcccggc agcggagagc acgttgaaat
      781 gttcaaggat ggctccgaag tgccgaacaa gcccatccat ttcttcgtcc tccacgcgga
      841 gtctggcgag atcggctaca tgccgcaccg cctttgtgtc cagcgcgcat gcgctagagt
      901 ctctgggtgt cattgcattc aaacctcctg ataagcagac ggcgaacgat agagcgcatc
      961 gctttcccaa acggattcag gaatctctca cgccgaaaaa aacggagaga aaagcttccg
     1021 ttctccttgt ttctcgaaga gaacgttcag gaacgctttg catagtattc caccggtctt
     1081 cccgatttcc tttcgatctg cttgaaatgc agcccgcggc gaatgaaggt tacgcttcgt
     1141 ccgtcaggcg ggtcatctca agaaattctt cttcatccac tacttttacg ccaagagaga
     1201 gcgccttcga aagcttgctc cccgcatcct ttcccgccac gacgaaagac gtcttcttgc
     1261 tgacgctcga agaacaaacg gcgcctcgac gttttgccat tttctccgcc tcgctgcgcg
     1321 tcatggactc catctcgccg gtaaaaacca ggcgcattcc cgcgaacggc cgggagccaa
     1381 taggttcgtt ttcccgctcg tcttcaagtc ggggaagcgt cgcccggact ccggccgcct
     1441 ctaatcgctc aagaagcctc tgattctcag gctgctcgaa gaacgatcga atggccgacg
     1501 ccattaccgg cccaacgccg tcgactgcgg caagagtctc ctcggatgcg tctcgaagcg
     1561 tcggcagatc acggaatacc tccgcaagaa tctccgcgct gcgcgaaccg gtaaaacgaa
     1621 tgccgagggc ggagagaagg cgtgaaaatg gacggtcttt cgattgtttc agtgcgttga
     1681 taagattcct tgcggacttc tcccccatcc gatcaagcga ggcaagcgtc tcttcgtcca
     1741 acgtatagag gtcggcaagc gaacggagca gccccttctc gacgagctga tcgacgatct
     1801 tgtctcccag cccccggata tccattccgc tgcgcgaggc aaagtgtctg agtccttcct
     1861 tgagctgcgc cgggcaggaa gaacggttcg gacagcgcag cgcaacctct ccttcaagac
     1921 gtactgcggg cgttccgcat gcagggcagc gctcgggcat aatgaactcc cgttcctccc
     1981 cggttcgcgc gtcaagcacc ggaccgagta tttccgggat gatctcgccg gccttgcgga
     2041 cgcgcaccat atcgcctatg cgtacatcct tgcgacggag ttcgtcttcg ttgtggagac
     2101 ttgccctccg cacgaccgag cccaaaagag taacaggcga gagaaccgca accggagtaa
     2161 gggcgccggt acgtccgacg gagacgacga tatcctccag gcgggt
//
LOCUS       DAOSWW010000438         2204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39860_length_2204_cov_3.680782, whole genome shotgun sequence.
ACCESSION   DAOSWW010000438 DAOSWW010000000
VERSION     DAOSWW010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39860_length_2204_cov_3.680782"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PK442_08905"
     CDS             <1..182
                     /locus_tag="PK442_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87810.1"
                     /translation="FDPRAGACGTLYDIVRDGRLNHRCAVRSGVLEGECAAMMTEYFR
                     NRRTVRKKNAVHERS"
     gene            188..1330
                     /gene="pseC"
                     /locus_tag="PK442_08910"
     CDS             188..1330
                     /gene="pseC"
                     /locus_tag="PK442_08910"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HOO87811.1"
                     /translation="MKTLSYGRQFIDDDDIDAVVAVLKGDWLTQGPSVKEFEERFAEY
                     VGTSYAVAFANGTAALHGAYFAAGVEAGDEVITSAMTFAATGNAALYLGGTPVFADID
                     PSTLCLDPKAASKKLSKRTKVIAPVSYAGYPVDIAPFLESAESCGAVVVEDACHALGA
                     ERNGVRVGKEAHMTAFSFHPVKHITTGEGGMVVTDSEEFARRLRLFRTHGIEKEPRFM
                     TAPPDGPWATEMQILGFNYRLSELHAALGLSQLRKLDRFVLRRKEIAALYDELFEGDR
                     VLCTPPRCEGHAYHLYPLQFSAGNRAAVFEELGKRGIRGMVHYLPVPLHPYYRERFGY
                     VPGEFPHAEELYAREISLPVYYGLTDDDIRRVAEEVRRIADSFPNR"
     gene            1430..1873
                     /locus_tag="PK442_08915"
     CDS             1430..1873
                     /locus_tag="PK442_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOO87812.1"
                     /translation="MFSGKRIGILLSLALLLICSSAVLASANEKIGVIDSQKIIFQHP
                     RFEAVTKQLQQISQKKENEMKMAVDKESDQNKKAQIFNTKRNEFAQEEAKLMQPIFQE
                     AQVAVRTVARLKGVTVVIEQSAVYFGGIDITDDVIQELKKKAAGK"
     gene            1996..>2204
                     /locus_tag="PK442_08920"
     CDS             1996..>2204
                     /locus_tag="PK442_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOO87813.1"
                     /translation="MRMKLLVTDIDETLSRGEQLTPGVISACERLRGNGWTIMVASGR
                     ILATALPHIRGTGTDLPAILYDGGR"
BASE COUNT          473 a          578 c          682 g          471 t
ORIGIN      
        1 gtttcgatcc ccgggccgga gcgtgcggca ctctctacga tattgtccgg gacggccgct
       61 taaaccacag atgcgcagtc cgctccggag ttctcgaagg ggagtgcgcc gcgatgatga
      121 cggagtattt caggaaccga agaaccgtcc gaaaaaaaaa cgctgttcat gagaggagct
      181 gatgtgaatg aaaacgctgt cgtacggccg ccagttcatt gacgatgatg atatcgatgc
      241 agtggtggcc gttctcaagg gagactggct cacccagggg ccttccgtga aggagttcga
      301 ggagcgcttc gcggagtatg tcggaacgag ctacgcggtt gccttcgcga acggaacggc
      361 cgctctccac ggggcatact tcgccgccgg agtggaagcc ggggacgagg tgatcaccag
      421 tgcaatgacc tttgccgcga cgggaaacgc cgcgctctat cttggcggca cgcccgtctt
      481 tgcggacatc gatccctcga cgctctgcct cgacccgaag gcagcgtcga agaagctgtc
      541 gaagcgcacg aaagtgatcg cgccggtgag ctatgccggc tatcccgtgg atattgcgcc
      601 ctttctcgaa tccgcggagt cgtgcggcgc ggtggtcgtc gaggatgcct gccatgcgct
      661 cggagctgag cggaacgggg tccgcgtcgg caaggaagcg catatgacgg ccttcagctt
      721 ccaccccgtc aagcacatta cgacgggaga ggggggcatg gtcgttaccg attccgagga
      781 gttcgcccgc cgtcttcgtc ttttcaggac gcacgggatt gaaaaagagc ctcgtttcat
      841 gaccgctccg ccggacggtc cgtgggcgac ggagatgcag atcctcgggt tcaactaccg
      901 tttgagcgaa ctgcacgccg cgcttgggtt gagccagctc cggaagctcg accgcttcgt
      961 ccttcggcgg aaggagattg cggcgcttta cgacgaactc ttcgaagggg atcgcgttct
     1021 ttgcacgcct ccgcgatgcg aggggcatgc ctaccacctc tatccgcttc agttctccgc
     1081 cggaaaccgc gccgccgtct tcgaagagct ggggaaacgg gggatccgcg gaatggtgca
     1141 ctaccttcct gtgccgctgc acccgtacta cagggagcgg ttcggttacg tccccgggga
     1201 gtttccgcat gcggaagagt tgtatgcgcg ggagatcagc ctgcccgtct attacggact
     1261 taccgacgac gatattcgca gggttgcgga agaagtccgc aggatagccg atagtttccc
     1321 gaatcggtag acggacgagg gattacgtaa atatgcctgg acttttgatt ggtttgggta
     1381 taaaattttc tttgtatcta tatttaccga ctgtcgagga ggtagtttaa tgttttcagg
     1441 aaaacgtatc ggtattcttc tttcgttggc tcttttgttg atttgttcct ctgctgttct
     1501 tgcgtccgcc aatgagaaga taggcgtcat agattctcag aagatcattt tccagcatcc
     1561 gaggttcgaa gccgtcacaa agcagcttca gcagatcagc cagaagaaag agaacgagat
     1621 gaagatggct gtggacaagg aatccgatca gaataaaaaa gcgcagatct tcaacacgaa
     1681 gcgtaacgaa ttcgcccagg aagaggcaaa gctgatgcag ccgatcttcc aggaggctca
     1741 ggtcgcagtg aggaccgtgg cgaggttgaa gggcgtcacc gtggtcatcg agcagtccgc
     1801 cgtctatttc ggcggtatcg acatcaccga cgacgtcatc caggagctga agaagaaggc
     1861 cgcaggaaag tagtttttcc gccggttgca gcacagtctg aaagtttgat tttcgaggga
     1921 gccgcttctc tccggtatgc ggagatacaa gcaagcggct ccctttttta ctactgttct
     1981 tcggaggaaa aagagatgag aatgaaactt cttgtaactg atatagacga gacgctttcc
     2041 cgcggggagc agctcacgcc cggtgtgatt tccgcctgcg agcgtctccg gggaaacgga
     2101 tggacgatca tggtagcttc ggggagaatt ctggcaaccg cgctgccgca tatccgggga
     2161 acggggacgg atcttccggc gatcctctac gacgggggac ggat
//
LOCUS       DAOSWW010000439         2204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39863_length_2204_cov_3.318288, whole genome shotgun sequence.
ACCESSION   DAOSWW010000439 DAOSWW010000000
VERSION     DAOSWW010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39863_length_2204_cov_3.318288"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PK442_08925"
     CDS             <1..685
                     /locus_tag="PK442_08925"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOO87814.1"
                     /translation="AIEDYFDRSFELEELLKSRNKQTLYDEVISLSEMADFIYLRQHR
                     PLGLGHAVLCGEPVCRNEYFGVILPDDVMVAEPSVLAQLLAVHSERGGSVVALEEVSA
                     EDTSRYGVVAAENAGNGVFAVKDMVEKPGPEKAPSRLAIMGRYILSPAIFPILERQQA
                     GAGGEIQLTDGIRTLISQEPVWGVVYKGRRFDCGTQPGWLHANIRLAMEHPALKEVVL
                     RAVKEYEEE"
     gene            905..>2204
                     /gene="glmS"
                     /locus_tag="PK442_08930"
     CDS             905..>2204
                     /gene="glmS"
                     /locus_tag="PK442_08930"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOO87815.1"
                     /translation="MCGIVGYIGHRNVADVLVKGLQSLEYRGYDSAGIAVINYPDEEQ
                     TLRTPAVHIVKGVGKVAELKSMLAAHPVSGHQGIGHTRWATHGGVTETNAHPHADLSS
                     RLVLVHNGIVENYRELRAELLVPEEQYKSETDTEVIAQLLSALYTGGGAQDMINALVA
                     LHGRLRGSFALVIMVKDIPGKIYCLRKGSPLVVGFSEEGEALCASDVPAILPYTQDVV
                     YLDDGDIAELSAEGMRFWSAAGESLEKSEVHIDWDVSMVDKGGYSHYMLKEINEQGAV
                     LRSSMARRLSGEGEVDLSSELPWTKEDALAWKRIHIVACGTSYYASLVAERFLETITD
                     LDIRVDVASEYRYRNLPVGPDTLAVFVSQSGETADTLAAERLAKARGARCLAVTNVIG
                     STLAREVDDILQLKAGPEIGVAATKTFMGQMAVLYLLSLYV"
BASE COUNT          461 a          619 c          687 g          437 t
ORIGIN      
        1 ggcaatcgaa gactacttcg accgctcttt tgaacttgaa gagctgctta agtcgcggaa
       61 caagcaaacc ctctacgacg aggtgatctc gctctccgag atggcggatt tcatctacct
      121 ccggcagcat cgtcctctcg gcctggggca tgcggtgctc tgcggggaac cggtctgccg
      181 caacgagtat ttcggcgtca tccttcccga cgacgttatg gttgcagaac cctcggtcct
      241 tgcgcagctt cttgcagtgc acagcgagcg gggaggcagc gtcgtcgctc ttgaagaagt
      301 ctctgcggaa gacacgtcgc gttacggcgt cgttgccgcc gagaacgccg ggaacggcgt
      361 tttcgcagta aaggacatgg tggagaagcc gggaccggaa aaagccccga gcaggcttgc
      421 tatcatggga cgctacatac tctctccggc aatcttcccg attcttgagc gccagcaggc
      481 gggagcggga ggagagattc agctgaccga cggcattcga acgctgatca gccaagagcc
      541 tgtctggggc gttgtgtaca agggaaggcg tttcgactgc gggacgcagc cgggatggct
      601 gcacgccaac attcgccttg ccatggagca ccctgcattg aaggaggtgg tgctgcgagc
      661 ggtgaaggaa tacgaagaag agtagacagc gcatgaacga agcgcctgcg ccggagaaca
      721 gctccccgga tccggggaat cgagtcttcg gctgacgagg acggaggagt atcgatctgt
      781 cggcggatgc ctccggggcc gtgagagacc ggcccgcaaa gcctttctgc aaaaccggaa
      841 agtaatttcc gggacaaagt gaaacgcagt ctctcgtgag tggatacaaa ggcggtgctg
      901 tataatgtgc ggaatcgttg gctatattgg ccaccgaaat gttgcggatg tacttgtaaa
      961 aggccttcag tcccttgaat acagagggta tgactcggcg gggatcgccg ttatcaacta
     1021 ccctgacgaa gaacaaactc tccggacgcc cgcggtgcat atcgtcaagg gcgtcggcaa
     1081 agtggccgag ctgaagtcca tgctcgccgc tcatcccgtt tccggccatc aggggatagg
     1141 gcatacccgg tgggcaaccc acgggggcgt caccgaaacc aacgcccatc ctcacgcaga
     1201 tctttcctcc aggttggtgc ttgtccataa cggaatagtg gagaactacc gtgaacttcg
     1261 cgccgagctt cttgttcccg aggaacagta caagtcggag accgacacgg aggtcatcgc
     1321 gcagcttctc tccgcccttt ataccggcgg cggagcacag gacatgatca atgccctcgt
     1381 cgccctccac ggacgcttga gaggttcgtt tgcgcttgtc atcatggtga aagacattcc
     1441 cgggaagatc tactgccttc gcaaaggctc gccgctggtc gtcggctttt cggaagaagg
     1501 ggaagcgctc tgcgcttcgg atgtgcccgc catacttccg tatacgcagg acgtcgtcta
     1561 tcttgacgac ggggatatcg ccgagctctc ggctgaagga atgcgtttct ggagcgccgc
     1621 gggggaatcg ctcgaaaaga gtgaagtgca tatcgactgg gatgtctcca tggtggacaa
     1681 ggggggctac tcccactata tgctcaaaga gatcaacgaa cagggggctg tgctcaggag
     1741 ctccatggcc cggcgcctct ccggcgaggg cgaagtggac ctctcctccg agctcccatg
     1801 gacgaaggag gacgccctgg cctggaagcg gattcatatt gtcgcctgcg ggacgtcgta
     1861 ctacgcgtcg cttgtcgctg agcgctttct tgagaccatc acggatcttg atatccgggt
     1921 ggacgtcgcg tcggaataca ggtacagaaa cctcccagtg gggccggata cattggcagt
     1981 cttcgtctcc cagtcggggg agacggccga tacccttgcc gcggaacggt tagcaaaagc
     2041 tcgcggagcc cgctgcctgg ccgttaccaa cgtcatcggt tccaccctgg ccagggaggt
     2101 ggacgatata ctccagctga aggccggtcc cgagataggg gtcgccgcga cgaagacctt
     2161 catggggcag atggccgtgc tctatctgct ctcgctctat gtcg
//
LOCUS       DAOSWW010000440         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39961_length_2201_cov_3.484623, whole genome shotgun sequence.
ACCESSION   DAOSWW010000440 DAOSWW010000000
VERSION     DAOSWW010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39961_length_2201_cov_3.484623"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..561
                     /locus_tag="PK442_08935"
     CDS             19..561
                     /locus_tag="PK442_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOO87816.1"
                     /translation="MDQVDDEQFTLERVIPELVEFVEKEMGSDIPVIGAGGIWGREDM
                     EKVFALGAKGVQIGTRFACTFEGDASDRFKQAFIDATEEDVVIIHSPAGLPGRALKSP
                     FVAQYLEGNVKSKPCIANCLTHCRYKTEKETFCIAQALIDSFRGNWEEGLFFCGSNVV
                     KVKEMQHVEDIMRDFFPEMA"
     gene            654..>2201
                     /locus_tag="PK442_08940"
     CDS             654..>2201
                     /locus_tag="PK442_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87817.1"
                     /translation="MKTTLRRCVVICAMLLAELFFCILIFGSAGSANPAEASQEAEKT
                     VLEVAKVNEEASVREIPLDKQAIQDRITDIQTELDALIEKKESLLETENIEEDDLRRT
                     LVALGSLQNVYSRLTSAQDNLTEAKREQEAQEKKSAEPVISGQPPFNLSFYEDVRNRY
                     ESVEQRLEAVNSSLRLSEGSVSTVDEQIRRMEEQHRVLSEELQNLKNASGVKQLLQVK
                     ESEADLETAKASRIVQDLNIEAQNISRNLLLAERDKLKTGLEFVKKHVAYDEKDRTAR
                     AERIGEQIAAAREKLPGLREDREKARTALSRSQGALTSARNERERVLAQAGVAEQQAA
                     LQLAQVSLEHTELTLLFLEEEQRIWNARYSLLNGSLKGQELWDMRSLIVTRVKDLESA
                     FLVRQRDVSSIQAEMHAAQKELEEHTGANAVLARIRARIDLLNRIAESANASMARIFS
                     LFNQYNRLREEINDHIDAVKIAERVTTFGKERFLAFWNITLWSGDGFDITVAKLVFAV
                     SLFLAAFF"
BASE COUNT          552 a          537 c          645 g          467 t
ORIGIN      
        1 catctcggag tgacgaagat ggaccaggtg gatgacgagc agtttaccct tgaacgagtt
       61 atccccgagc ttgttgaatt cgttgagaaa gagatgggta gcgatatccc ggtcatcggc
      121 gcgggcggca tatggggccg cgaagatatg gaaaaggtct tcgccctcgg agctaaagga
      181 gtgcagatcg ggacacggtt cgcctgtacg ttcgaggggg atgcttcgga caggtttaag
      241 caggcattca tcgatgcgac cgaagaggac gttgttatca ttcatagccc cgccggtctt
      301 cccgggcgtg cgctcaaatc tcccttcgtc gctcagtacc tggaagggaa cgttaagagt
      361 aaaccatgca ttgcgaactg tctgacgcac tgcaggtaca agacggagaa agaaactttc
      421 tgcatcgcgc aggccttgat cgactccttc cgcggcaact gggaggaagg gctcttcttc
      481 tgcggatcaa acgtcgtgaa ggtaaaggaa atgcagcacg tcgaagacat aatgagagac
      541 ttctttcccg agatggcata attcggactc gccggagcgt ttctgccgcg cttttccttt
      601 tcaaggcggg agcttgctcc cgccttttct gatagaaaga ggaggagaac acgatgaaga
      661 ctaccctgcg tcgttgcgtt gtgatctgcg caatgctctt ggcagaactg tttttctgca
      721 tcctgatttt tgggagcgca ggctctgcga accctgccga agcatcgcag gaagcggaaa
      781 aaacggttct ggaggttgcg aaggtcaacg aggaggcctc cgttcgtgaa attccattgg
      841 acaagcaggc tatacaggat cgcatcactg atattcagac ggagttggac gctctcatcg
      901 agaagaaaga gagcctcttg gagactgaga atattgaaga ggatgatctg cgaaggacgc
      961 ttgtcgctct cggctctctt caaaacgtct attccaggct gacgagcgcc caggataatc
     1021 ttacggaagc gaagcgggag caagaggcgc aggagaagaa gtcggcggag cccgtcatta
     1081 gcggacaacc gcccttcaac ctctcattct acgaagacgt ccgtaaccgg tacgagtctg
     1141 ttgagcagcg tcttgaagca gtgaattctt ctctccgtct ttccgaagga agcgtttcaa
     1201 cagtagatga gcagatacgg aggatggaag aacagcacag agtcctttcc gaagaattgc
     1261 agaacctgaa aaatgcttcc ggcgtgaagc aattgctcca ggtaaaggaa tccgaggctg
     1321 atcttgagac cgctaaggcg tcccggatag tccaggattt gaacattgaa gcgcaaaaca
     1381 ttagccgcaa tctgcttctc gccgaacgcg acaaactgaa aacaggattg gagttcgtta
     1441 agaaacatgt agcgtacgac gagaaagacc ggacggcgcg ggccgagcgc atcggcgagc
     1501 agatagcggc ggccagagag aagctccccg ggctgcgcga agatagagag aaggcgagaa
     1561 ccgccctctc aaggagccag ggagcgctga cttcagcaag aaacgagagg gaaagggtgc
     1621 ttgcgcaggc tggagtagcc gagcagcagg ctgcgcttca actcgcgcag gtttctctgg
     1681 aacatacgga actgacgctg ctctttctcg aagaggagca gcggatatgg aatgccaggt
     1741 attctctcct caacggaagt ctcaaggggc aggaactttg ggatatgcgc tcgctcatag
     1801 tcacccgcgt gaaggatttg gagagcgctt tcctagttcg tcaaagggat gtttcctcca
     1861 ttcaggcgga aatgcacgcg gcccaaaaag aactggagga acacactgga gcaaatgccg
     1921 ttcttgcaag aatacgtgcg aggattgatc tgctcaaccg catagccgag agcgcgaacg
     1981 cctcgatggc ccggattttt tcgcttttta atcagtacaa ccgactccgt gaggagatca
     2041 acgatcatat cgacgcggtg aagattgcgg aacgagttac caccttcggc aaggagcgtt
     2101 ttttggcgtt ttggaacatc actctctggt ccggagacgg gttcgacatc acagtggcaa
     2161 aactggtttt cgcggtctcc ctcttcctcg ctgcgttctt t
//
LOCUS       DAOSWW010000441         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39966_length_2201_cov_3.027959, whole genome shotgun sequence.
ACCESSION   DAOSWW010000441 DAOSWW010000000
VERSION     DAOSWW010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39966_length_2201_cov_3.027959"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PK442_08945"
     CDS             <1..854
                     /locus_tag="PK442_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87818.1"
                     /translation="TGPNFALPIGIVCGLLGLVTSIELDLMGMAWFLTSCAIAIPLAA
                     IVGYGYGKLMNAVKGSEMTIATYTGFSIVALMCLAWLMIPFKNPKMGWFIGTGLRETI
                     QLDAVGGAQILNNFLMFTIGEVIIPTGLLLTFAFFSFLVWLFFRSKRGIAISAGGINP
                     KFARATGLDIDGNRVFANVISTVLGAIGIIVYSQSYGYAQLYTAPLMMAFPAVAAVLI
                     GGATASRAKVSHVIIGVVLFQGLLTTALPVANEVFAGTDLSEIMRMIIQNGIILYALT
                     QVKRGVQ"
     gene            854..2143
                     /locus_tag="PK442_08950"
     CDS             854..2143
                     /locus_tag="PK442_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87819.1"
                     /translation="MESLHNRSKGAGEWILENIVTLIFIAFTLFGFMVSSGVSVGYFF
                     SELSSRIFRNSFLVLSLIIPVLAGLGLNFGIVIGAMSGQVAISIARYFEMGGISGLLF
                     CFLVAALVAALCGYFTGKLYNKTRGQEMIASLIVGFFANGIYQFLFLFVVGVVIAVPA
                     THPMIKPDGIGIRMTVDLVPVKQGGLKYALENIYQIPFVHALLIIALGLLALLLIRYY
                     WNKRKGYGYRNSMSSLIVNGTLCLALAGVAIHAIMTKSELMMVREVPVVTGLLIIGLC
                     VFTVLIMKTKLGQDFRSVGQSQHTAEVSGINVDRTRIIATMISTVLAAWGQIIYLQNM
                     GTLNTYNAHTQIGMFSVAALLVGGASTSKASITHAIFGTILFNAMFIMSPEIGQSLFG
                     QAILGEYFRTFMVYGVIGVALGLHVWNTNKKSRISVE"
BASE COUNT          421 a          622 c          628 g          530 t
ORIGIN      
        1 gaacggggcc gaacttcgcg ctgccgatag gcatcgtctg cgggctgctc ggactggtca
       61 catctattga gttggacttg atgggaatgg catggtttct gacgtcgtgc gccatcgcga
      121 ttccgctcgc cgcgattgtc ggctacggat atggcaagct gatgaacgcg gtgaagggat
      181 ccgagatgac gattgccacc tatacggggt tttcgattgt cgcgctgatg tgtctcgcgt
      241 ggctgatgat cccgtttaaa aaccccaaaa tgggctggtt catcggcacc ggactcagag
      301 agacaattca gctcgacgcg gtcgggggcg cgcagattct caataatttc ctgatgttta
      361 ccatcgggga agtgatcatc ccgacggggc ttcttctgac gttcgcgttt ttttcatttc
      421 tcgtatggct cttcttccgc tcgaaacggg gaatcgcgat ttccgcggga ggaatcaacc
      481 cgaagttcgc gcgggctacc ggccttgata tcgacggcaa cagggtcttc gcaaacgtga
      541 tctccacagt tctgggcgcg atcggcatca tcgtctactc ccagagctac ggatacgcgc
      601 agctgtacac ggcgccgttg atgatggcgt tcccggcggt tgccgcagtc ctcatcggag
      661 gggccaccgc gtcgcgcgcc aaagtatccc atgttattat cggcgttgtc ctcttccagg
      721 gcttgctgac cacggcgctt cccgtggcga acgaagtatt tgccggtacc gacctgtcgg
      781 agataatgag aatgattatt cagaacggaa tcatcctcta cgccctgacc caggtgaaga
      841 gaggtgtgca ataatggaaa gtttacacaa cagatccaag ggtgcaggcg agtggattct
      901 tgagaacatc gtcacgctga tttttatcgc attcacgctt ttcggcttca tggtgtcgag
      961 cggcgtctcc gtcggctact tcttctcgga gctttccagc aggatcttca gaaattcttt
     1021 tctcgtcctt tcgcttatca ttcctgtact tgcgggcctc gggctgaatt tcggcatcgt
     1081 catcggggcg atgtcggggc aggttgccat atccatagcg cgctacttcg agatgggcgg
     1141 catttcgggg cttctgttct gtttcctggt ggcggcgctt gttgccgcgc tctgcgggta
     1201 cttcactgga aagctgtaca acaagacgcg gggtcaggag atgatcgcca gcctgattgt
     1261 cggcttcttt gcgaacggca tctatcagtt tctcttcctc ttcgtcgtcg gcgtcgtcat
     1321 cgctgttccc gcgacgcacc cgatgatcaa gcccgacggc atcggcatcc gcatgaccgt
     1381 cgacctcgtg ccggtgaagc agggcgggct gaagtacgcg cttgaaaaca tctaccagat
     1441 cccgttcgtc cacgcgctcc tgatcattgc cctcggtctt cttgcgctgc tgctcattcg
     1501 ctactactgg aacaagagaa aagggtacgg gtacaggaac agcatgtcgt ctctcatcgt
     1561 caacgggacg ttgtgccttg ctctcgcggg cgtcgctatc cacgcaataa tgacgaaatc
     1621 ggagcttatg atggtgcgcg aggttcccgt cgtgacgggg ctgctcatca tcggcctttg
     1681 cgtcttcacg gtgctcatca tgaagacgaa gctggggcag gatttccgtt ccgtcgggca
     1741 gagccagcac acggcggagg tctccggaat caacgtcgac cggacgagga tcatcgctac
     1801 tatgatctcg accgttcttg cggcctgggg gcagatcatc tacctccaga acatggggac
     1861 gctgaacacc tacaacgcgc acacccagat aggcatgttc tccgtcgcgg cccttctcgt
     1921 gggcggcgcc tccacctcga aggcgagcat cacccatgcc atcttcggca ccattctgtt
     1981 caacgccatg ttcatcatgt cgcccgagat agggcagtcg ctcttcggtc aggcgatcct
     2041 cggagaatac ttcagaacct tcatggtgta cggcgtcatt ggcgtggctc tcggactcca
     2101 cgtctggaat acgaacaaaa agagcaggat ttccgtggaa taaccggcgg ggggtattgc
     2161 ttcactgaga caacacaacg cgcccgtctt tcctgaaccg g
//
LOCUS       DAOSWW010000442         2200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_39986_length_2200_cov_3.888578, whole genome shotgun sequence.
ACCESSION   DAOSWW010000442 DAOSWW010000000
VERSION     DAOSWW010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39986_length_2200_cov_3.888578"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            724..>2200
                     /locus_tag="PK442_08955"
     CDS             724..>2200
                     /locus_tag="PK442_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87820.1"
                     /translation="MGAKPRRSFAETLYSLPLLGYCAELLVQLVRLPVVLREVNIRQT
                     RKDTVVQSRLDGIGNRVAALREGASLCRREMEAALSGKADADTLASISEDLSKKVDAE
                     RFDSFTLNLEAELSRKVSAESLEDLGASWETALSELREELSGKAGKDSLRAMENELQT
                     KANAGSTAAIEEKVERIIERLGALDGSAVLDRLESMELLLEGKADIEEVAKLLSAKLD
                     TDALEIWGKRLEDASAQLLEALSQKADVERVDALSESLRSKADGETLESLSAAKADVE
                     ELLSLKARFESLGAHTSELENTLHSLCASKADLDAVRSLGERFEGVAAQLGALEGESV
                     LNRLSSAESAIERKADLEIVKSLLSLKMDVEVLKSLGKSLEEASGQTFAALEKKADLH
                     SVESVASTLDLKADAESVRSLNEGLSVVNERLGDFASKEDFSKVGELERLLSRKADAD
                     ALEERLSALDAKADLKTVEEKLAQKADAEAVESLSKEFEQRL"
BASE COUNT          483 a          558 c          667 g          492 t
ORIGIN      
        1 cgtatcgtgt ttctcgccgt cggacgaacg tatcaggtag cgaacttctc cccgaggagc
       61 gtgaaaagag gagctccttt gaagaattga gctccccttg ctttccaagt gttggcttct
      121 tgtgaagtct atgatactct tgaacagtgt ccaagccatt cattctgaag gttatcgctt
      181 ttcggatggc gccgccgcgt ggaaaggaga atttcgggag tgaacgaaaa aaagaataca
      241 attttttttg atgggggcgg cgatccatct cctgctgttc atgacgaaga gagcgctctg
      301 cttttttcgt ctctaccccg aagctcctcg gacgcaggga gaccgaatgt ctccggcgat
      361 tcccaatctg cgcctctccc cgatacccgg acggatacga cttccgaact gcccgtcgtc
      421 atacggcggg aacccgcgag ttccccgaat gctgcggaca attctcttcc tccgtccgga
      481 cagagcgctc aaccgcaaaa agggcaggct ctgattgaac gtctccgtca tgaacttgcc
      541 gtacggcggc gggaagattc tgtatatcct tcaacgcgcc gcccccgcct ggaaaagaag
      601 gcgggccggg tttctccgca gacagcttcc gtccactctc aacccccgaa aacagctcct
      661 gtgcaagcga aacgcgcagc tccctcttgg aactcacgga aatctgaagg gaagccatcc
      721 tttgtgggcg ccaagccccg gcgttccttt gcggaaacac tgtactcgtt acctctgctc
      781 ggttactgtg ccgaactcct tgtccagcta gtcaggcttc ccgttgttct ccgcgaggtg
      841 aatatccggc agaccaggaa ggatactgtt gtgcagtcgc gacttgacgg tatagggaac
      901 agggtcgccg cgctccggga gggagcttcg ctctgccgcc gggaaatgga ggcggcgctc
      961 tcgggcaaag cggatgccga cactttggct tctatctcgg aggatttgtc aaagaaagtt
     1021 gacgcggagc ggtttgacag ctttaccttg aatcttgagg cggaattgtc tcgaaaggtt
     1081 tccgcggagt ctcttgagga tttgggcgct tcatgggaga cggctttgag cgagctcagg
     1141 gaggaacttt ccgggaaagc cggcaaggac agcctgcgcg caatggaaaa cgagttgcag
     1201 acgaaggcga atgccgggag tacagcggcg attgaggaga aagtggagcg cattattgag
     1261 cgtctgggcg ccctcgacgg aagtgcggtt ttggatcgtc tggaatccat ggaattgctt
     1321 ctcgaaggaa aagcggatat tgaggaagtc gcgaagctgc tttcagcaaa gctcgatacg
     1381 gacgctctgg aaatctgggg aaaacgcctg gaggacgctt ccgcccagtt gttagaagcg
     1441 ctttctcaaa aggctgacgt ggagagagtg gatgcgcttt ccgagtcgct gcgctcgaag
     1501 gcggacggag aaacgctcga atcgttgtct gcggcaaaag cagatgttga agagcttctc
     1561 tctttgaagg ctcgtttcga atcccttggc gcacacacct ccgaactcga gaataccttg
     1621 cattctcttt gtgcgtcgaa agcggatttg gatgctgtgc gttctcttgg cgagcgtttc
     1681 gaaggagtag ccgctcagct tggcgcgctc gaaggagaga gcgtcttgaa ccgattgagc
     1741 tcggcggagt cggcaatcga acgtaaggct gatctggaga ttgtgaaatc gctgctctcg
     1801 ctcaagatgg acgtcgaggt gctgaaatcg cttggaaaaa gcctggagga ggcgtccggc
     1861 cagacgttcg ccgcacttga gaaaaaagcg gatcttcaca gcgtcgagtc tgtggcctcc
     1921 acgcttgatc tgaaagcgga tgcggagtcg gtacgctcct tgaacgaggg gctttcggtt
     1981 gtgaacgaac gtctcggcga ttttgcttcc aaagaggatt tttcaaaggt tggggagttg
     2041 gagcggctgc tttccaggaa ggcggacgct gatgcgcttg aggaaagact ctctgcgctt
     2101 gacgcgaaag ccgacctgaa gacggtggag gagaagctcg cgcagaaagc cgacgctgaa
     2161 gctgttgaat cactctcgaa ggaatttgaa caacgtcttg
//
LOCUS       DAOSWW010000443         2198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_40040_length_2198_cov_3.238451, whole genome shotgun sequence.
ACCESSION   DAOSWW010000443 DAOSWW010000000
VERSION     DAOSWW010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40040_length_2198_cov_3.238451"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /gene="rimM"
                     /locus_tag="PK442_08960"
     CDS             complement(<1..465)
                     /gene="rimM"
                     /locus_tag="PK442_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOO87821.1"
                     /translation="MNRVRIGRIVGTHGLRGALKIVPLTDYPERFHSMEKMSVYSAEG
                     QIITTLTLLSSRYSDHKKLLVIQTEELRRIEDAEPLSGAFIEILPEERYPLGEGEYWI
                     EDLKGLSAVRHEDGASLGKLVDVATAGENDIYVIRDDSGNDHYIPAVKQFVAE"
     gene            complement(458..706)
                     /locus_tag="PK442_08965"
     CDS             complement(458..706)
                     /locus_tag="PK442_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOO87822.1"
                     /translation="MPDYRELVEFIVRRLVTQPDEVQISEQTDERGVTAITIRVAPED
                     VGRVIGKRGATINAIRLVAKASAVKVNDRVDVDIVEDE"
     gene            complement(709..984)
                     /gene="rpsP"
                     /locus_tag="PK442_08970"
     CDS             complement(709..984)
                     /gene="rpsP"
                     /locus_tag="PK442_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOO87823.1"
                     /translation="MAVRIRLARHGRKKAPFYRLVVADSRSPRDGRFIELLGTYDPMK
                     DPSSINVDEERAMHWLKVGATASDTARSILRKSGVWEKFEAGKKQAE"
     gene            complement(1064..>2198)
                     /locus_tag="PK442_08975"
     CDS             complement(1064..>2198)
                     /locus_tag="PK442_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOO87824.1"
                     /translation="VAAIVYEELAELMGKEPVPLVISPKPPTVYLMVGLQGGGKTTST
                     VKIARKLSGAHKPLVVACDLRRPAAVEQLRVLAAQSKISFFGPENGEKNVLNVVKAAL
                     SYADDHLNDVVLLDTAGRLHADEDLMDELVAMKKSAPPTEILLVVDAMTGQEAVKVAS
                     SFHEKLQLTGVVLSKLDGDARGGAALAIRKVTGVPVKLAGVGEATDALEVFDAKRMAQ
                     RILGMGDVIGLAEKIQEMADSGGAERMAETLKKKFTLEELLAQFEQIEKMGPLNKVLE
                     MIPGFSKMKGVSQDEVDSGRLKKSKAIIQSMTRAERRDPSIIKGSRRRRIAMGSGTSV
                     QMVNQLLAQYEQMRKLWKQFGKQGKGFKLPKGLLGGGGGFRFK"
BASE COUNT          454 a          661 c          537 g          546 t
ORIGIN      
        1 ttcggcgaca aactgtttca ccgccgggat atagtggtca tttccggaat cgtcgcgaat
       61 tacataaatg tcgttttcgc cggcagtcgc cacatcgacc aattttccaa gagacgcgcc
      121 gtcttcatgc cggacagccg aaagtccctt cagatcctct atccagtatt ctccttcgcc
      181 gagcggatac cgttcctcgg gaagaatctc gatgaaagcc ccggaaagag gttctgcatc
      241 ctctattcga cgcagctcct cggtctgaat cacaaggagt ttcttgtggt cggagtatcg
      301 gctggacaga agcgtgagcg tggtgataat ctgtccctct gcgctataca ccgacatttt
      361 ttccattgag tgaaatctct ccggataatc ggtcaggggt actatcttga gcgcccctct
      421 cagcccgtga gttccgacaa tgcgtccgat gcgtactcta ttcatcctcg acgatatcga
      481 cgtcaacccg gtcgttcact ttcactgcgg aagcttttgc taccaggcga atcgcgttga
      541 tcgtcgcgcc gcgtttcccg atgacccttc cgacatcctc cggagcgact cgaatggtga
      601 tcgccgtgac gcccctttcg tccgtctgct cactgatctg aacctcgtca ggctgtgtca
      661 cgaggcgtcg gacgatgaat tcaacaagct cccggtagtc gggcatgact actccgcctg
      721 tttcttgccc gcttcgaact tttcccagac cccggacttt ctcaaaatgc tcctggctgt
      781 gtcggaagcc gtcgcgccga ctttcagcca gtgcatcgcc cgctcttcgt ccacattgat
      841 tgaagaggga tctttcattg ggtcgtaggt tccaagaagt tcgatgaagc gaccgtctct
      901 gggtgaacga gaatccgcca ccaccaaccg ataaaaagga gcctttttcc gtccgtgacg
      961 ggcaaggcga atacgtactg ccatttacca tacacctcct gatattgttc cggaacacat
     1021 cgaatacatt gcttccccgg agtgctccgt caccgggaac gttctattta aaacgaaaac
     1081 cgccgccgcc tccaaggagg cctttcggta gtttgaagcc cttcccctgt tttccgaact
     1141 gcttccatag cttccgcatc tgctcgtact gggccagaag ctgatttacc atctggaccg
     1201 atgttcccga gcccatcgca atccgacgtc tgcgactgcc cttgatgatc gaaggatccc
     1261 tccgttcggc gcgagtcatc gactgaatga tagccttcga ttttttcaac cgccccgaat
     1321 cgacttcgtc ctgggacacg cctttcatct tgctgaatcc ggggatcatc tcaaggactt
     1381 tgttcagggg ccccattttc tcaatctgct cgaactgcgc aagaagctcc tcaagtgtaa
     1441 atttcttctt cagcgtctcg gccatgcgct cggccccgcc ggaatcggcc atctcctgaa
     1501 ttttttccgc caggccgatg acatcgccca ttccgagtat tctctgggcc atcctcttcg
     1561 catcgaagac ttcgagagcg tccgttgcct ctccaacacc ggccagcttg accggcactc
     1621 cggtcacctt gcgaattgcg agggcggcgc ctccgcgggc gtctccgtcg agctttgaga
     1681 gaacgactcc tgtcagttgg agtttctcat gaaatgaaga ggcaaccttt accgcctcct
     1741 ggccggtcat ggcgtcgacg acaaggagta tctcggtagg aggagcggac ttcttcatgg
     1801 cgacaagctc atccattaaa tcctcgtcgg catggagacg tccggccgta tcaagaagca
     1861 caacgtcgtt cagatggtca tccgcatagc tcagagcagc ttttacgacg ttgaggacat
     1921 ttttttctcc attttcagga ccaaaaaatg aaattttcga ttgtgcagcc agtacccgaa
     1981 gctgctcgac agcggcgggg cgacgaaggt cgcaagcaac cacgaggggt ttatgcgccc
     2041 cggaaagctt tcgcgctatc ttcaccgtgc tcgttgtttt tcctccgccc tgcaaaccga
     2101 ccatgaggta gacagtcggc ggctttggag aaataacaag gggtacaggt tcttttccca
     2161 tcaactctgc aagttcttca tagacaatcg ccgcgacg
//
LOCUS       DAOSWW010000444         2179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_40605_length_2179_cov_2.741055, whole genome shotgun sequence.
ACCESSION   DAOSWW010000444 DAOSWW010000000
VERSION     DAOSWW010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40605_length_2179_cov_2.741055"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK442_08980"
     CDS             <1..761
                     /locus_tag="PK442_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOO87825.1"
                     /translation="LKDPEKRQKYDTLGPNWQQGQDFSPPPGWGGGVHMDFGGDGGGF
                     SDFFRTIFGGFASGGRGVSMDDLFRSQGRGGDEEMSLELSLEDILRGGTKTISMEQVV
                     HGTDGRPSRSRRTLNVNLPQGVTEGSRIRLKGQGSRGADLYIVIHAAPHPKFTVEGYD
                     LVTSLKVSPWEAALGASIPVTTLDGVVSMKLPAGVQAGKRLRLKEKGLPRRKGAQAGD
                     LIVKVEVVVPEKLSDRERELFEALAKESSFNPRG"
     gene            complement(745..>2179)
                     /locus_tag="PK442_08985"
     CDS             complement(745..>2179)
                     /locus_tag="PK442_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO87826.1"
                     /translation="ETTLDPALQELLERTLSVSLESLPEEITAAGIVMDNDSGDVLAY
                     VGNGRLGRGIAGSWIDCARRPRSPGSALKPFAYLAAFEKGILTPASLLADTPLAFSGQ
                     APRNFDLTYRGPVSARIALADSLNVPAVRVLRAAGPELVLDLLRNAGFLSLTQSTAHY
                     GDSLILGGCETSVLQMAEGYVMLATLGIRRPPRFLPSEPPFQRRLVSEGAAFLVADIL
                     KDTGRLLPLHGKRIEYGKEWFAFKTGTSYGYRDAWTAAYTPRNTVVVWMGNPTGKAHP
                     ELVGSQAAAPAIVEILRGLPPSGWYDPPQQVIMRTVCALSGRPIAPACPVGRLDYALE
                     GISSSVPCPMHVIRDGSARTVWPAELEEFALRRSLTLQAEQKATITSPLPGSRYILTP
                     LGGDQRVALKAEGAKPPVYWFVGGEYAGKQEGKEALFWTLKKGVHAVSLVDAAGRTAV
                     SRFTVDAPGVKGPDESGPPPLLLNPGD"
BASE COUNT          410 a          656 c          725 g          388 t
ORIGIN      
        1 tgctcaagga tcctgagaaa cggcagaagt acgacactct tggtccgaac tggcagcagg
       61 ggcaggattt ttcgccgcct cccggatggg gcgggggcgt gcacatggat tttggcggcg
      121 acgggggcgg gttcagcgac ttcttcagaa cgatcttcgg cggcttcgcc tcgggcggca
      181 gaggcgtttc gatggacgat ctcttccgtt cgcaggggcg tggcggcgac gaggagatgt
      241 ctctcgaact ctcgcttgag gatattctcc ggggagggac gaagaccatt tcgatggagc
      301 aggtggtgca cggcacagac ggacgtccgt cccgaagccg gaggacgctc aacgtgaacc
      361 ttccccaggg cgtgaccgag ggttcgcgca tcaggctcaa agggcaggga agcagaggcg
      421 ccgatctgta catcgtcatc catgcggcgc cccatcccaa gttcaccgtc gaggggtacg
      481 atcttgtcac ttccctgaag gtctctccat gggaggccgc ccttggagca tccatccccg
      541 tgacgacgct cgacggcgtg gtctcgatga agcttccggc aggagttcag gcgggaaagc
      601 ggctccgttt gaaagaaaaa gggctcccca gaagaaaggg agcccaagcg ggcgatctta
      661 tcgtgaaagt cgaggtcgtc gtgcccgaga aactgagcga ccgcgagcgg gagttgttcg
      721 aagcgctcgc aaaagagtcg tcgttcaatc cccggggttg agaagaagcg gcggagggcc
      781 ggattcgtcc ggtcccttca cgcctggagc gtcgacggta aaccgcgaaa cggcggtcct
      841 tccggcggcg tccaccagcg aaacggcgtg tacccctttc ttgagcgtcc agaagagcgc
      901 ttcctttccc tcctgttttc cggcatattc tcctcccacg aaccaataga ccgggggctt
      961 ggcgccctcg gccttcaacg ccaccctctg atcccctccg agcggtgtga gaatataccg
     1021 cgagccgggg agcggagagg tgatcgtcgc tttctgctcc gcctggagcg tcagcgaacg
     1081 gcgcagagca aactcttcca gttcggcggg ccagactgtc cgtgccgatc cgtctctgat
     1141 gacgtgcatc gggcacggca cggaggacga aataccctca agagcgtagt ccaaccttcc
     1201 tacagggcag gcgggagcga tcggccggcc cgatagagcg cacaccgtgc gcatgataac
     1261 ctgctgcgga gggtcgtacc accccgaggg agggaggccg cggaggatct cgacgatggc
     1321 gggagcggcg gcctgcgagc caacgagctc cgggtgcgct ttccctgtgg gattgcccat
     1381 ccagacgacg acggtgttgc gcggcgtata ggcggcggtc caggcgtcgc ggtagccgta
     1441 cgaggtacct gttttgaagg cgaaccactc tttcccgtac tctatccgct tgccgtgcag
     1501 ggggaggagc cgtccagtgt ctttgaggat atcggcgacc agaaacgccg ctccttcgga
     1561 gaccagcctc ctttggaatg gaggttcgct cgggagaaaa cgcggcgggc ggcgtatccc
     1621 cagcgtcgcc agcatgacgt acccttccgc catctgaagt acgctcgttt cgcaccctcc
     1681 aagaatcaga gaatctccat aatgggccgt gctctgcgtc aggctcagga aacccgcgtt
     1741 gcgaaggagg tcgagcacca gctccggccc cgccgcccga agtaccctga ctgccgggac
     1801 gttgagcgag tccgcgaggg ctatcctggc tgaaaccggt cccctgtagg tcaggtcgaa
     1861 gttccgcggc gcctgtccgg agaaggcgag cggagtgtcc gccaggagcg acgcgggggt
     1921 gagaattcct ttctcgaatg ctgcaagata ggcgaacggc ttgagcgcgg agccggggga
     1981 gcgagggcgg cgggcgcagt cgatccagct tccggcgatt cctcttccga gtcgtccgtt
     2041 gcccacatag gccaaaacat cgcccgaatc gttgtccatc acgattccgg cggcggtgat
     2101 ctcctcgggc agggactcca gggagacgga cagcgtcctc tcaagaagct cctgaagcgc
     2161 ggggtcgagc gtcgtctcc
//
LOCUS       DAOSWW010000445         2178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_40628_length_2178_cov_3.716439, whole genome shotgun sequence.
ACCESSION   DAOSWW010000445 DAOSWW010000000
VERSION     DAOSWW010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40628_length_2178_cov_3.716439"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PK442_08990"
     CDS             <1..238
                     /locus_tag="PK442_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOO87827.1"
                     /translation="SVHPLDAAFFDLKEGDEAVARGRRRGALRVVVREDASLGRGYCV
                     LPQGTEGVNSLTEPLASPGFGAPFAESWITLGKG"
     gene            269..1102
                     /locus_tag="PK442_08995"
     CDS             269..1102
                     /locus_tag="PK442_08995"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO87828.1"
                     /translation="MRFVLYLVAGYAVLVTIAFFFADKLLFFPHRELWATPSERGLPW
                     EDVRFEAADGTPLHGWYVPGAGRGAGGAAKATVLFCHGNAGNISHRIDSLAIFHRLGV
                     STFIFDYRGYGESEGTPSEQGVYSDVEGAWSWLTKERGVPPEQIILFGRSLGGAVAAY
                     AASRGEKFSPRGLILESTFTSLASVGQSHYFFLPMKLIVGNAFDTLSRLERIDCPVLV
                     ASSPEDEVVPGRHGRILFEAAREPKWFLPLKGDHNLGYMTTGRAYVDGLDAFFRNVLG
                     E"
     gene            1131..>2178
                     /locus_tag="PK442_09000"
     CDS             1131..>2178
                     /locus_tag="PK442_09000"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidE/YbjL duplication"
                     /protein_id="HOO87829.1"
                     /translation="MEFFESLLKNHLFLMSLTVALGILVGKISVRGINLGISGALFVG
                     LFIGAQGYSVPNEYFAWNLMFFVVAVGLLAAEDTARVVKRYGLKFILLSVAVTGAGAL
                     ATLAAGILFSGSASPNMIAGTYTGALTSSPGLGAALEATGGSPDVTIGYSVAYPFGVM
                     AVVLFVQLIPAIFGIDVAKEREKLALERKAAEPASPDARPASIPFSLLSFVICIIGGS
                     VLGGISLPLGPLGSVGLGTTGGALLFALGVGSFDHIGPLQMRMEKNVLSAVRAISLGF
                     FLAVVGLNAGGGVVKAFLEHGVLLIAVGIGSALAAEMVGFFLGHYVWKINWIILAGAI
                     CGAMTSTPGLGAAID"
BASE COUNT          321 a          657 c          749 g          451 t
ORIGIN      
        1 ctcggtgcac ccgctggacg cggcgttctt tgacctgaag gagggcgacg aggccgtcgc
       61 gcgcggcaga cgacggggag cgcttcgcgt cgtcgtccgg gaggacgctt cgctcgggcg
      121 ggggtactgc gtgctcccgc aggggacgga gggcgtcaac tcgctgaccg aaccgctggc
      181 gagccccggt ttcggcgcgc cgttcgccga aagctggatc acgctcggca aggggtagca
      241 ggtcccggta aggaggtttc ccgtgcgcat gcgttttgta ctctatcttg tagcaggata
      301 tgcggtgctt gtgacgatcg cttttttctt cgcggacaaa ctgctcttct ttccgcaccg
      361 cgagctttgg gcgacgccct ccgaacgggg gcttccgtgg gaagatgtgc gcttcgaggc
      421 cgcggacgga accccgctcc acggctggta cgtgccgggc gcagggcggg gggcgggcgg
      481 cgcggcgaag gcgacggtgc tcttctgcca cggcaacgcg gggaatatct cgcaccggat
      541 cgattcgctg gcgatctttc accggctcgg cgtctccacc ttcatcttcg actacagggg
      601 gtacggagag agcgagggaa cgccctccga acagggggtt tacagcgacg ttgaaggtgc
      661 gtggagctgg ctgacgaagg agcgcggcgt tcctcccgag cagatcattc tcttcggcag
      721 gtcgctcggc ggcgcggtgg cggcgtacgc ggcatctcgc ggcgaaaagt tctccccgcg
      781 agggctgatt ctggagtcca cctttacctc gcttgcctcg gtcgggcaga gtcactactt
      841 ctttcttccg atgaagctga tcgtggggaa cgcgttcgac accctctccc gactcgagcg
      901 gatagactgc ccggttcttg tggcgtcgag cccggaggac gaagtggtcc ccggcagaca
      961 cggcagaatt ctcttcgagg cggcgcgcga gccgaagtgg tttctccctc tgaaaggaga
     1021 ccacaacctc ggatatatga cgacggggcg ggcgtacgtc gacggattgg acgctttctt
     1081 ccggaacgtc ctcggcgagt aagttcacaa cagataagga gatgtttcca gtggagtttt
     1141 tcgagtccct tctcaagaat cacctcttcc tgatgagcct gacggtcgca ttgggcattc
     1201 tcgtcgggaa gatcagcgtc agaggcatca acctcgggat atcgggggcg ctcttcgtcg
     1261 gcttgtttat cggcgcacag gggtactccg ttccgaacga atatttcgcg tggaatctga
     1321 tgttcttcgt ggttgcggtg ggcttgctgg ccgccgagga taccgcgcgg gtcgtcaagc
     1381 gctacgggct caagttcatc ctgctctccg tcgccgtcac gggcgcgggg gcgctcgcca
     1441 cgctcgccgc cggaattctc ttctccggct cggcgtcgcc gaacatgatc gcggggacgt
     1501 acaccggcgc gctcaccagt tcgccggggc tcggtgcggc gcttgaagcc accgggggaa
     1561 gcccggacgt caccatcggc tactcggtcg cgtacccctt cggcgtcatg gcggtggtgc
     1621 tcttcgtgca gctcattccg gcgatcttcg gcatcgacgt ggcgaaagag cgggaaaagc
     1681 tcgcgcttga gcgaaaggcg gcggagccgg catctccgga cgcgcgtcca gcttcgatcc
     1741 cgttttcgct gctctccttc gtgatctgca tcatcggcgg ctccgtattg ggcgggattt
     1801 cgcttccgtt gggaccgctc ggttcggtcg ggcttgggac gacgggcggt gcgctgctct
     1861 tcgccctcgg cgtcggcagc ttcgatcata tcggcccgct gcagatgcgg atggagaaaa
     1921 acgtcctctc ggcggtgcgc gccatctcgc tcggcttctt cctcgcggtg gtggggctga
     1981 acgctggggg cggcgtggtg aaggcctttc ttgagcacgg ggttctgctc atcgccgtcg
     2041 gtatcggctc ggcgctggcg gcggagatgg ttggtttctt ccttgggcac tacgtctgga
     2101 agatcaactg gatcatcctc gccggggcga tctgcggcgc gatgacgagc acccccggcc
     2161 tgggcgccgc catcgacg
//
LOCUS       DAOSWW010000446         2178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_40644_length_2178_cov_2.349976, whole genome shotgun sequence.
ACCESSION   DAOSWW010000446 DAOSWW010000000
VERSION     DAOSWW010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40644_length_2178_cov_2.349976"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PK442_09005"
     CDS             complement(<1..579)
                     /locus_tag="PK442_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOO87830.1"
                     /translation="MRLVLYNIRYGTGTGWSYHLPFPFSGCLRRTEGRFRRISEYVSK
                     LNPDLVGLVEADSGSFRQNGSCQAESLASRIGGESVFACKYDSGSIVSKTPLLKSQGN
                     AVITKLHMIGTKEHILSKGVKKTLLEVEFDDFTLFLTHLPLGYAARKVQLGEIAERCI
                     DATKPVILAGDGNTYRGERELRSFFRKTGFQNA"
     gene            791..>2178
                     /locus_tag="PK442_09010"
     CDS             791..>2178
                     /locus_tag="PK442_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOO87831.1"
                     /translation="MVVKVPPEPFRIKMVEPVRVPGKDEREKALEVARFNMFGLRGAD
                     VYIDLLTDSGTGAMSKQQWAAIMMGDEAYAGADSFFNLKKAVKEVLGFDYTIPVHQGR
                     AAENVVFGSLVKEGDVIPFNMPFDTTRGHIFNCKASSVDCVIDEAFDRTNQHPVKGNV
                     DIAKLEKAIAEYGKARIPFVMVTITNNSGGGQPVSLANLREVSAVAKKHGIPVLLDAA
                     RMAENAWFIKNREKECARMSIAEILKATMDTADAITVSCKKDPLVNIGGLVACRTEEM
                     YYKIVPRVILFEGFATYGGLAGRDLEALAVGLHEMTDEQHLTHRIAQVRYLGDLLEEG
                     GVPCIQPIGGHAVFIDAAAFLPHIPQGSYPADVLSVEIYREGAIRGIGLGALAFETTD
                     EKTGERIFPKLELFRLAVNRRTYTNSHIEYVAESIVNVYKRRDSIRWGLEMVYEPPVK
                     GLKHFLAHLKPVA"
BASE COUNT          492 a          687 c          602 g          397 t
ORIGIN      
        1 ggcgttttga aaaccggtct tccggaagaa cgagcgcagt tcccgttcgc ctctgtaggt
       61 attgccgtcg ccggccagga tgaccggctt cgttgcgtcg atgcaccgct ccgctatctc
      121 gccgagctgc accttccgcg cggcgtaccc gagggggaga tgcgtcagga agagcgtgaa
      181 atcgtcgaat tcgacctcca gcaacgtttt tttgactccc ttgctcaaaa tatgttcctt
      241 cgtgccgatc atgtgaagct ttgtgattac ggcgttcccc tgcgatttca acagcggcgt
      301 cttcgagacg atggagccgg agtcgtactt gcacgcgaaa accgactcgc cgccgatccg
      361 cgaggcaaga gactccgcct gacaggagcc gttctgacga aacgatccgg aatccgcctc
      421 aaccagcccg acaagatcgg ggttcagttt ggagacatac tccgagatac ggcggaagcg
      481 cccctccgtc ctccgaagac atcccgagaa aggaaacgga aggtgatagc tccatccggt
      541 tccggttcca tacctgatgt tgtacaacac taaacgcata tgagcctccc taaacacaga
      601 aaatatcgtt cattatataa cacctccgcc attcctctac ggtgattcct gctgtttctc
      661 caaacctccc ggcaggcact gcaaaaagtt tttcaccgat gatgcgaaaa aaatagcaaa
      721 acaggagaat atagggtaga ataacggaag aaacctgatc ttttctcaat tgaaaaggag
      781 gaaaaaattc atggtcgtga aggtaccccc cgagcctttt cgcatcaaga tggtagaacc
      841 ggtccgcgtt cccggcaaag acgagcgcga gaaagcactt gaagtggccc gtttcaacat
      901 gttcggtctc cgcggcgcgg acgtctatat cgacctcctc accgactccg gaacaggcgc
      961 gatgagcaag cagcagtggg ccgccatcat gatgggcgac gaggcctacg ccggcgccga
     1021 cagcttcttc aacttgaaga aggcagtcaa ggaagttctc ggcttcgact acaccattcc
     1081 cgtccatcag gggcgcgcag ccgaaaacgt cgtcttcggc tcgctcgtca aggagggcga
     1141 cgtcatcccc ttcaacatgc ccttcgacac caccaggggg cacatcttca actgcaaggc
     1201 gtcctccgtg gactgcgtga tcgacgaggc cttcgaccgc accaaccagc acccggtcaa
     1261 ggggaacgtc gacatcgcga agctcgaaaa agccatcgcg gagtacggca aggcgcgcat
     1321 ccccttcgtc atggtcacca tcacgaacaa ctcgggcggc ggtcagccgg taagcctggc
     1381 gaatctcaga gaggtctccg ccgtcgcgaa gaaacacggt attcccgtgc tcctcgacgc
     1441 ggcgcgcatg gccgaaaacg catggttcat caagaaccgc gaaaaagagt gcgcgcggat
     1501 gagcatcgcg gagattctga aggccacaat ggacacggcc gacgccatca ccgtctcgtg
     1561 caagaaggat ccgctggtga acatcggcgg cctcgtcgcc tgccgcaccg aggagatgta
     1621 ctacaagatc gtccccagag tcatcctctt cgagggcttc gccacctacg gcggcctcgc
     1681 gggacgcgat cttgaggcgc tcgcggtcgg cctccatgag atgaccgacg aacagcacct
     1741 cacccaccgc atcgcgcagg tccgttacct cggcgatctg ctcgaagagg gcggcgtgcc
     1801 ctgcattcag ccaatcggcg gccatgcggt cttcatcgac gccgcggcct tcctgccgca
     1861 cattccgcag gggagctacc ccgccgacgt gctctcggtc gaaatctacc gcgaaggcgc
     1921 catccgcggc atcggcctcg gcgcgctcgc cttcgagacc acggacgaga agacgggcga
     1981 gcggatcttc ccgaagctcg agctcttccg tctcgcggtt aaccgccgca cgtacactaa
     2041 cagccacatc gagtacgtcg cggagagcat cgtcaacgtg tacaagcggc gcgattcgat
     2101 ccgctggggg ctcgaaatgg tctacgaacc gccggtgaaa ggcctgaaac acttcctggc
     2161 ccacctgaag cccgtcgc
//
LOCUS       DAOSWW010000447         2174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_40756_length_2174_cov_2.700802, whole genome shotgun sequence.
ACCESSION   DAOSWW010000447 DAOSWW010000000
VERSION     DAOSWW010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40756_length_2174_cov_2.700802"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PK442_09015"
     CDS             <1..301
                     /locus_tag="PK442_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOO87832.1"
                     /translation="RAMRYGPGFRYPVIGPLQTADLGGLDTFYYISSYLFASLSDMKE
                     PSVTLKNLMETQQLGVKTKSGFYDYSNGKDEETIRRRDKMFFKMLKHIHSNGKNS"
     gene            339..1235
                     /locus_tag="PK442_09020"
     CDS             339..1235
                     /locus_tag="PK442_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO87833.1"
                     /translation="MKGKENKKDESGWSEKQRDSLDGRLIDHYIYVLSRLSGMQVADV
                     GVCICDLDGVVFYKPARSLDLKVKHGDEIRPGSLLYRALHERRRVTAKMDSSLYGVPY
                     VGVGTPIMDEGGQVLGAVAITESTLNYENLKESSMKIAESIEMLAGASEEIAAQSQEM
                     SGTAAIMKETLERSRESVKASDQVIGLIKMIATRTNLLGLNASIEAARVGEQGRGFSV
                     VAEEIRKLAGVTGDSIKNIAHTLASIQSDNAENCRQMLVMSSIITQVSDATTQIAETA
                     QALNEMASSLNEMAEKIMESIN"
     gene            complement(1248..>2174)
                     /locus_tag="PK442_09025"
     CDS             complement(1248..>2174)
                     /locus_tag="PK442_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOO87834.1"
                     /translation="TGDAMDIQHLKYFVQVIKNQSFTKAAEKLVVTQPMLSRVINQLE
                     EELDVKLIERTSKHFKTTDAGELLYQQALKVLHTHNDIYQYINDLKSAKSGEVLISIP
                     GVLLDMYFPPLLFEFHQKYPNVKLDIIEEGSKLTAQSVLSEQVELGFVMLPVRNASKL
                     TSRIVTTSICHVLVNKEHRFATRNTPIHTSELIDERIITFSTTATLHDAFIRHCENEG
                     FEPNIVYKTLMPNFIFEMVHLGLCIGILPYPVVKRFIFDGLVSVPLEPKIIWEIAVIS
                     KKDNYLSFAARNFLSFVEEYSVRKGKEWQGLE"
BASE COUNT          568 a          451 c          613 g          542 t
ORIGIN      
        1 ccgtgccatg cgttacggtc ccggattccg ctaccctgtc atcggtcctc ttcagacggc
       61 tgatttggga ggtttggata cgttttatta catctcttcg tatctcttcg catctttaag
      121 cgatatgaag gaaccttcgg ttacattgaa aaacttgatg gaaacacagc agttgggagt
      181 aaaaaccaag agcggatttt acgactactc caacggtaag gatgaagaaa ccataaggcg
      241 aagggataag atgtttttca agatgctgaa acatattcat tcaaacggga agaatagctg
      301 aaaaatacct ttggaatgtg aaaagagtgt tgcagcatat gaagggtaaa gaaaataaga
      361 aggatgaaag cggatggagc gaaaaacagc gtgattcgct cgatgggagg cttattgatc
      421 actatatata cgttctgtcc cgattgagtg gaatgcaggt tgcggatgtc ggcgtctgta
      481 tctgtgatct tgacggcgtg gttttctaca agccggcgcg ctccttggat ttgaaggtca
      541 aacatggcga cgagattcgc cccggaagtc tcttgtatcg agctctgcat gagcggcgga
      601 gagtaacggc gaagatggac tcatctcttt acggggtgcc gtacgtaggg gtaggcaccc
      661 ctatcatgga cgaaggcggt caagttttgg gagctgttgc aataacggaa tcgaccctta
      721 actatgaaaa cctcaaagaa tcttctatga agatagcgga aagcattgag atgctggcag
      781 gggcttccga ggagatcgcc gcgcaatctc aggaaatgag cggtacggcc gcgataatga
      841 aggaaacgct tgaaaggtcc agagaaagcg tgaaggcaag cgatcaggta atagggctta
      901 tcaagatgat agcgacgcga acgaatcttt tagggttgaa cgcatccata gaggcggcga
      961 gggttggcga acagggaaga ggctttagcg ttgtcgcgga agagatcaga aaacttgccg
     1021 gcgttacggg cgactcgata aaaaatatag cgcacacgtt ggcatccatt cagtccgaca
     1081 atgcggaaaa ctgccggcag atgctcgtca tgtcttcgat aattactcaa gtgtccgatg
     1141 cgacgacgca gatcgccgag accgcgcaag cgttgaacga gatggcgagc agtctgaacg
     1201 aaatggcgga gaaaattatg gaatcgataa actaaggacg gcgcgtttca ttcgagtcct
     1261 tgccactctt tcccctttct cacggaatat tcttcgacga atgagaggaa gttccgcgcg
     1321 gcgaacgaaa ggtaattgtc ctttttactg atgaccgcta tttcccagat aattttcggt
     1381 tccagcggta ccgagacgag cccgtcgaaa atgaatcgtt tgacaacagg gtatggaagt
     1441 atccctatgc aaaggccgag gtgcaccatc tcgaaaatga aattgggcat gagcgtttta
     1501 tatacgatat tgggctcgaa gccttcgttc tcgcagtgtc ggataaacgc gtcgtgcagg
     1561 gttgccgtcg tgctgaaggt tatgatgcgc tcatcgataa gttccgacgt gtggatcgga
     1621 gtgtttcttg tggcgaaacg gtgctcttta tttacaagga catggcagat gctcgtggtg
     1681 acaatacggc ttgtcaactt agaggcgttg cgaacaggga gcatgacgaa ccccagttca
     1741 acctgctcgg acaagactga ttgcgccgta agcttggagc cctcttcgat aatgtcgagc
     1801 ttgacattcg ggtatttttg atggaactcg aagagaagag gggggaagta catgtccagg
     1861 aggacgccgg ggatgctgat caatacttcg ccggattttg cggatttaag atcgttgatg
     1921 tactgataga tgtcgttgtg ggtatgcagc actttcaacg cctgctgata caggagttct
     1981 ccggcgtccg tcgttttgaa gtgtttgctg gttcgttcta tcagtttgac gtcgagttcc
     2041 tcttcgagct ggttgatgac ccgtgaaagc atcggttgtg tgaccacaag cttctcggcg
     2101 gccttggtga aactctgatt ctttataacc tgaacaaagt attttaaatg ttgaatatcc
     2161 atggcgtctc cagt
//
LOCUS       DAOSWW010000448         2172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_40799_length_2172_cov_3.303732, whole genome shotgun sequence.
ACCESSION   DAOSWW010000448 DAOSWW010000000
VERSION     DAOSWW010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40799_length_2172_cov_3.303732"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PK442_09030"
     CDS             complement(<1..837)
                     /locus_tag="PK442_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOO87835.1"
                     /translation="MPYASSGKKFLEALRDSAGEDRWNDWRWQMQSRITTADELGRIL
                     TLSRGEGATLRKSLNSLRMAITPYYASLIDPDDPECPIRKQAVPTLKETFIAPTDLLD
                     PLHEDVDSPVPGLTHRYPDRCILLVTDQCGMYCRHCTRRRFAGQTDTARSSEQIRACI
                     DYIAHTPEIRDVLITGGDPLTLSDEALDSILSEIRAIPTVEIIRIGTRTPAVLPMRVT
                     ERLCAVLKKYHPLWINLHFNHPRELTPEAARACALLADAGIPLGNQSVLLKGVNDCPY
                     IFR"
     gene            complement(953..1420)
                     /locus_tag="PK442_09035"
     CDS             complement(953..1420)
                     /locus_tag="PK442_09035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO87836.1"
                     /translation="MSNDLAERCLKTAAATGAFTADELDVLEDVLVEWSLHPGRDYIL
                     LTEWIDGDIAGFLIYGPTPMTRFAYDLYWIAVAPHHQKKGLGGTLEEKMCSALLEQSD
                     SAVIRVETAGRDDYLGQRYFYLARHYGECGRIPDFYSEGDDLVLYCKKLTKKR"
     gene            complement(1413..>2172)
                     /locus_tag="PK442_09040"
     CDS             complement(1413..>2172)
                     /locus_tag="PK442_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOO87837.1"
                     /translation="GHLQFRSLVEEMSIPCTGNPSGVLQTCLSKETCSRTLRKAGIPV
                     PEGVSLHPGFDPELLASLRLPVFVKPLMEDGSVGIDASSFVQEKSALLRTVLEKLSLF
                     PRGVRAEEFIPGREYSVACIGNGPYNVLGVSVIDYEQTEGTNFLDYDSKWDPASPLYS
                     LVPQRAEGAFREKAAQLAAAAGRALGCRGYFRVDMRERHGILYVLDVNPNPDITPDGG
                     FLRQCREAGFSDEQTVHTILELAFEEDEGGTTRE"
BASE COUNT          426 a          637 c          661 g          448 t
ORIGIN      
        1 gcggaagatg tacggacagt cgttcacgcc cttgaggagc accgactggt tgcccagggg
       61 aatacctgcg tcggcgagaa gagcgcaggc gcgggcggcc tcgggggtga gctcccttgg
      121 atggttgaaa tgcaggttga tccagagagg gtggtatttt ttcagcacgg cgcacagccg
      181 ctctgtcact cgcatcggca gcaccgccgg cgttcgcgtt cctatcctga tgatctcgac
      241 cgtaggaatt gcccggatct cggagaggat ggaatccaga gcctcgtcgg agagcgtgag
      301 gggatcgccg cctgtgatga gcacgtcgcg gatctccggc gtatgcgcta tgtagtcgat
      361 gcatgcccgt atctgttcgg aggaacgggc tgtgtcggtc tgtccggcaa aacgccttct
      421 ggtgcagtgc ctgcagtaca tgccgcactg gtccgtgacc agcagaatac acctgtcggg
      481 gtagcggtgc gtcagccccg gtaccggaga atccacatcc tcgtgcagcg ggtcaagcaa
      541 atcagtcggc gcgatgaacg tctccttaag cgtcggcacg gcctgtttcc tgatcgggca
      601 ttccgggtca tccggatcga tcagcgacgc atagtacggg gtaatcgcca tacggagcga
      661 gttcaggctc ttccgcagcg tcgcgccctc tccgcgcgat agcgtgagaa tacgacccag
      721 ttcatcggcc gtcgtgattc gcgactgcat ctgccagcgc cagtcgttcc atcgatcttc
      781 ccctgcagaa tctctgagcg cttcgaggaa tttcttcccg ctcgaagcgt acgggatcaa
      841 gggagcgttt gtcccggcga gaagcgaaag ctggggaaaa gcatccctag tgcgatggcc
      901 tttcgacgga ggttttgttt cctccggcga ggcatccggt atgttgacga tattaacgct
      961 ttttcgtgag ttttttgcag tagaggacaa gatcgtcacc ttccgaataa aagtcgggaa
     1021 tgcggccgca ttctccgtaa tgccgtgcaa gatagaagta gcgctgtccc aggtaatcgt
     1081 cccttcccgc cgtttcgaca cggataaccg cgctgtccga ctgctcaaga agggccgagc
     1141 acatcttctc ctcaagtgtg ccgccgaggc cttttttctg atggtgagga gctacggcga
     1201 tccagtagag gtcataggcg aaacgtgtca tcggggtagg accgtagatc aggaacccgg
     1261 caatgtctcc gtcgatccac tccgtgagca ggatataatc gcgccccggg tggagcgacc
     1321 actcgaccag cacgtcctcg agaacgtcca gttcgtcggc ggtgaaggct cctgttgccg
     1381 ctgcggtctt caggcagcgc tcggcgagat cgttactcac gtgtcgtccc tccttcatct
     1441 tcttcaaaag ccagttcgag aatagtgtgc acggtctgct cgtcggaaaa ccccgcctcc
     1501 cggcactggc gcaaaaaccc tccgtccggc gtgatatcgg ggttcggatt tacgtcgagc
     1561 acgtagagaa tcccgtgccg ttcacgcatg tccacccgga aatagcctct gcagccaagg
     1621 gctcggccag ccgccgccgc gagctgcgcg gctttctccc ggaacgcgcc ctccgcccgc
     1681 tgcgggacaa gcgagtagag aggcgaggcg gggtcccatt tagaatcgta atcgagaaag
     1741 ttcgttcctt cggtctgttc gtagtcaatg accgagacgc caagcacatt gtacgggccg
     1801 ttcccgatgc aggcgacgga gtactcgcgt ccgggaatga actcctccgc ccggactccc
     1861 cgaggaaata gtgagagttt ctccaggacc gttcgaagca gcgcgctctt ttcctgcacg
     1921 aacgaggagg cgtcgatgcc tacgcttccg tcctccatca ggggtttgac aaacaccggc
     1981 aagcgaagcg atgccaagag ttcaggatcg aatcccgggt ggagcgagac cccttcgggg
     2041 acaggaattc ccgccttcct gagcgtgcgc gagcacgttt ctttcgagag gcatgtttgc
     2101 aacacccccg acggattgcc ggtgcagggg atggacatct cctcgacgag cgaccggaac
     2161 tggagatgtc ca
//
LOCUS       DAOSWW010000449         2170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_40835_length_2170_cov_4.423641, whole genome shotgun sequence.
ACCESSION   DAOSWW010000449 DAOSWW010000000
VERSION     DAOSWW010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40835_length_2170_cov_4.423641"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..384)
                     /locus_tag="PK442_09045"
     CDS             complement(61..384)
                     /locus_tag="PK442_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87838.1"
                     /translation="MTNGARKNVRSTWSGSADSSARVLGQSAYLLFRKVDEFGDEGKR
                     HALIQHAPDRLELRQFDAFFASFFPTFLPTFFSPLFSSFFFEFPDFLLQIGQHFVLYS
                     PASSA"
     gene            690..935
                     /locus_tag="PK442_09050"
     CDS             690..935
                     /locus_tag="PK442_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87839.1"
                     /translation="MNDSKTLAILLEFVQSPLVEGNYHIYKFDPQRVEKSDPELAEQI
                     VRLGEATERALEHLAENGIHVIAGGALPPYLTSSMLN"
     gene            1022..2113
                     /locus_tag="PK442_09055"
     CDS             1022..2113
                     /locus_tag="PK442_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87840.1"
                     /translation="MKKLYGTLLLSLCLLLLSNAAGNAAETLDNRLAELRSIENRGDL
                     DHLGLEKLRTLSGTEPKDILSLPQADILWLLSYGLDMYDSMLYSLVGKSERGARRQDE
                     KLGEFTDRFNTIWNTINSAYTALSEKSTEPSPADKLFDLLKKGASPQELLPVTTEGLK
                     YTWEIVKGWFAESEKEEAFLTELKTLRAENAALLENAPDSPWNFALLSYARLRRQSAD
                     YVKYLNQFEIEIARHPVPYEKTRVLNDGILSIKQELAAYDAKLRKAVVDIPVFTVQFY
                     SKARIDSAKGIFSDDTAAVLEAGALFARTAPLINLAVDNNVRKGWTEIASALKDLLNE
                     EQYDELKSISRAENMMWDFDDVPRTINWP"
BASE COUNT          539 a          644 c          616 g          371 t
ORIGIN      
        1 ccacgcgaat aagcagctgg tctacaacaa cgcccgcgtg gccgcgcagc tcgccgtggc
       61 ctacgcggaa gaggcggggg agtagaggac gaagtgctga cctatctgga gaaggaaatc
      121 cggaaactca aaaaagaagg acgagaagag gggcgagaag aaggtcggga ggaaggtcgg
      181 gaagaaggac gcaaagaagg cgtcgaactg gcgaagctcc agacggtccg gcgcatgctg
      241 gataagggca tggcgctttc cctcgtcgcc gaactcgtcg accttccgga agagcaggta
      301 cgccgactgg ccgagcaccc tcgctgaact gtcggccgaa ccgctccagg ttgacctgac
      361 gttttttctt gcgccgttcg tcaagaggtt tcccgcattc gggctcttga cgaacggcgc
      421 ggctattgct actattaatt tccatttgtt gtatatctct cattaccctc tcaggcagac
      481 ggctttcgga ccatccttcc cggcggagga tgaagcggag caagaaaatt tccagatctc
      541 actgaacgat gcacacgacc caaaaacact ggacatgatg tgtgttcggt tcccgcaacg
      601 tccgtcgtta ctcttcacgc gcacagcttc ggttcgggcg gaacgacgcc ctgaagcacc
      661 ttgataagag aacggaggag gtatcccgaa tgaacgattc caagacgctc gcgattttgc
      721 tggagttcgt ccaaagcccg cttgtggaag gcaactacca catctacaag ttcgacccgc
      781 agagagtgga aaagagcgac cccgaactgg ccgagcagat cgtccgtctc ggggaagcca
      841 ccgaaagagc cctggaacac ctcgcggaaa acggcatcca cgtgatcgcc gggggcgcgc
      901 tgccccctta cctcacgtcg agcatgctca actgaagcca cgtgaaaccg gagcgggaaa
      961 gagatgcttc tcccgctccg gaggttcaat gagtaaaaat caccttcagg aggaatgcgt
     1021 tatgaaaaaa ctgtacggaa cgctgcttct ctccctctgc ctgctgctcc tgtcgaacgc
     1081 agcaggaaac gccgccgaaa ctctggataa caggctcgcc gaacttcgca gcatagaaaa
     1141 ccggggcgat ctcgaccacc tcggcctgga aaagctaaga acgctcagcg gaacggaacc
     1201 gaaagacatc ctctccctgc cgcaggcgga tatcctctgg ctcctctcct acggcctcga
     1261 tatgtacgac tccatgctct actccctcgt cgggaaaagc gaacgcggag cgcgcagaca
     1321 ggacgaaaag ctcggcgaat tcaccgaccg gttcaacact atctggaaca cgatcaactc
     1381 ggcgtacacc gcgctttcgg aaaaatcaac ggaaccttcg cccgccgaca agctcttcga
     1441 cctgctgaaa aagggcgctt ccccgcagga gctgcttcca gtaaccaccg aaggcctgaa
     1501 atacacgtgg gagatagtca aggggtggtt cgcggaaagt gaaaaagaag aggcgttcct
     1561 taccgaactg aagaccctta gagccgagaa cgcggcgctt ctggagaacg cgccggacag
     1621 cccgtggaac ttcgcgctgc tgagctacgc cagattgcgg cggcaatccg ccgactacgt
     1681 caagtatctg aaccagttcg agatcgaaat cgcccgacac cccgtcccct acgagaagac
     1741 gcgcgttctc aacgacggca tcctctcgat caagcaggag ctggccgcat acgacgccaa
     1801 gctgcggaag gccgtcgtcg acatccccgt attcaccgtc cagttctact ccaaggcgcg
     1861 tatcgacagc gcgaaaggaa ttttcagcga cgacacggcg gccgtactgg aggccggggc
     1921 cctcttcgcc cggactgcgc ccctgatcaa cctggccgtg gacaacaacg tccgaaaagg
     1981 gtggacggag attgcttccg cgctgaaaga cctgctgaac gaggaacagt acgatgaact
     2041 gaagtccata agccgggcgg agaacatgat gtgggacttc gacgacgtcc ccaggacaat
     2101 caactggccg taaactaagg gcggggcgcg gaaggggctt ttgccgagct catcatacat
     2161 gatacccccc
//
LOCUS       DAOSWW010000450         2168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_40917_length_2168_cov_2.307619, whole genome shotgun sequence.
ACCESSION   DAOSWW010000450 DAOSWW010000000
VERSION     DAOSWW010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40917_length_2168_cov_2.307619"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PK442_09060"
     CDS             <1..760
                     /locus_tag="PK442_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOO87841.1"
                     /translation="MRLPYVVIEPNHALFLEAKKKGLRLLLGDPGQDAVLEAACVEKA
                     KLLVLTLHGALEIQDVVSAVRKRNRTLRILARDVSGEGGEFPDEDREISRIIQPEFEA
                     GLEMARQALIHLDVPGATIHRELETLRRNVYGPLRENNADYGLLSQLLHAASTLELEW
                     EAIHKDGPLEGKTIAGANIRAEFGVSVVAVLRDGALLPNPGGDFILREGDLVGIIGTM
                     EQNQKFFCFLDEQNERCRCASAEPPKTEKGENEK"
     gene            757..1806
                     /locus_tag="PK442_09065"
     CDS             757..1806
                     /locus_tag="PK442_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOO87842.1"
                     /translation="MISSQRIEDYGIRIGRMPKGLRNTIADVPGVAVGHCTLADGPVQ
                     TGVTAVIPAPGNLFREKVMAACHVINGFGKSCGIVQIEEMGTLETPILLTNTFGVGAA
                     SEALVRSMLAANPDIGRETGTVNPAVFECNDGFLNDIRALPVRGEHVLRAIASAGADF
                     EEGAVGAGRGMSCYQLKGGIGSASRIVECGGASFTLGALVLSNFGQMEDLTVDGRKVG
                     ASIFGIAPPEDEQGSIIALLATDVPLTERQLKRVARRASVGITRTGAFIGNGSGEIAV
                     AFTTANRVFHDETAPVRQIGMFNENEINLLFRAAVEATEEAVLNSMVRAEAVTGRDGH
                     TRRSLAEFAELFAER"
     gene            1995..>2168
                     /locus_tag="PK442_09070"
     CDS             1995..>2168
                     /locus_tag="PK442_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO87843.1"
                     /translation="MRVLDKESPVPLYYQLKEILQAMVDDGELAPGDIVPSERDICER
                     FSISRMTASKAVSA"
BASE COUNT          430 a          699 c          687 g          352 t
ORIGIN      
        1 catgcggctt ccctacgtcg tcatcgagcc gaaccacgcc ctctttcttg aggcaaaaaa
       61 gaaaggactg cgacttcttc tcggagatcc cggacaggac gcggttctgg aagcggcctg
      121 cgtcgagaag gcaaagctcc ttgtcctgac gctccatggg gctctggaga ttcaggacgt
      181 ggtcagcgcg gtccggaagc gcaatcgcac tctccggatt ctggcccgcg acgtcagcgg
      241 cgaaggcggg gagttccccg acgaggaccg agaaatttcg cgcatcatcc agcccgagtt
      301 cgaagcgggg cttgagatgg cccggcaggc gctcatccac cttgacgtac cgggcgcgac
      361 gatccacagg gagctggaga cgctccggcg gaacgtctac ggcccgctgc gggagaataa
      421 cgccgactac ggccttctct cgcagctgct gcacgccgca tcgacgctgg aactggagtg
      481 ggaggccata cacaaggacg gaccgctcga gggaaagacc atcgccgggg cgaacatcag
      541 ggccgagttc ggcgtctcgg tggtggcggt gctccgcgac ggcgcgcttc tgcccaaccc
      601 cggaggcgac ttcatactgc gggaaggaga tctcgtcggc atcatcggca cgatggagca
      661 gaaccagaaa tttttctgct tcctcgacga gcagaacgaa cgatgccgct gcgcatcagc
      721 agaacctccg aaaacagaaa aaggagagaa cgaaaaatga tatcttcaca acgcatcgag
      781 gactacggca tccgcatcgg ccgtatgccc aaaggactgc ggaatacgat cgccgacgtc
      841 cccggcgtcg ccgtcggaca ctgcaccctc gccgacggcc cggtacagac cggtgtgacc
      901 gccgttattc cggcgccggg gaacctcttc cgcgaaaaag tcatggcggc gtgccacgtg
      961 atcaacggtt tcggcaagtc gtgcgggatc gtccagatcg aggagatggg cacgctcgag
     1021 acgccgatcc tgctgaccaa caccttcggc gtgggcgcag cgtccgaggc gctcgtccgc
     1081 tctatgctcg ccgcgaaccc cgacatcggc agggagaccg ggacggtgaa cccggcggtc
     1141 ttcgagtgca acgacggctt cctgaacgac atccgcgcgc ttccggtccg cggggagcac
     1201 gtgctgcggg ccatcgcctc ggcgggcgcc gacttcgagg agggcgcggt cggcgcgggg
     1261 cgcggaatgt cgtgctacca gctcaaaggg ggaatcggct cggcgtcgcg catcgtcgag
     1321 tgcggcggcg cgtcgttcac gctgggcgcg ctcgtgctct ccaacttcgg ccagatggaa
     1381 gacctgaccg tggacggacg gaaggtcggc gcctccatct tcgggatcgc ccctccggag
     1441 gacgagcagg gatcgatcat cgcgctgctc gcgaccgacg tccctctcac ggagcggcag
     1501 ctcaaaaggg tcgcccggcg cgcctcggtc ggcatcacgc gcaccggcgc cttcatcggc
     1561 aacgggagcg gcgagatcgc cgtcgccttc acgaccgcca accgcgtctt ccacgacgaa
     1621 acagctcctg tgcggcaaat cggcatgttc aacgaaaacg agatcaacct cctcttccgc
     1681 gccgcagtgg aagcgaccga ggaagccgtg ctcaactcga tggtccgcgc cgaggccgtc
     1741 acggggagag acggacacac caggcgctcc ctcgccgagt ttgccgagct gttcgcggaa
     1801 cgataaccac atcgcctttt gattgcaccc cgaaggctgg agcaaacaca cgcaaggctt
     1861 ggggaggaac tcgcagtggt cctcttttta aagagtcttg tcttctttcg acacttcgct
     1921 tgacgcactc gccgccgacg tggtatagtc cacataacat gcagacaact ctacctctcg
     1981 gggggaaatt gcccatgcgc gtactggaca aagagagccc ggttccgctc tactaccagc
     2041 tcaaagagat tctgcaggcg atggtcgacg acggggagct tgcccccggc gacatcgtcc
     2101 cgtccgagcg cgacatttgc gagcggttct ccatcagccg catgacggcg agcaaggccg
     2161 tctccgcg
//
LOCUS       DAOSWW010000451         2163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41054_length_2163_cov_3.483871, whole genome shotgun sequence.
ACCESSION   DAOSWW010000451 DAOSWW010000000
VERSION     DAOSWW010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41054_length_2163_cov_3.483871"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..293
                     /locus_tag="PK442_09075"
     CDS             39..293
                     /locus_tag="PK442_09075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOO87844.1"
                     /translation="MKFYDLDSLGEKQGLDNKYILTAAVAARARSLSEQKGRTLEEDN
                     EKFISTALRDFDLGLVRLSSDERESEEAPVAVVSDAHVEE"
     gene            274..1491
                     /gene="coaBC"
                     /locus_tag="PK442_09080"
     CDS             274..1491
                     /gene="coaBC"
                     /locus_tag="PK442_09080"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOO87845.1"
                     /translation="MPTWKNKVKIVLGITGGIAAYKAPELVRTFVKSGCEVEVVLTED
                     GEKFVSPMVLSTLAGRRIWRQRDFLSDEYGWRIPHISLADWADVVVVAPCTAETLANT
                     VQGRGKELLGSLLLATRAQVLLCPAMNVNMLSNPATAENIRTLKNRGYAVVDPDSGDL
                     ACGYEGKGRLPSPYVLLEEMWRVACPKKELQRKNVLVTAGPTWEFLDPVRFLSNPSTG
                     KMGVAVARAAWYRGASVTLVLGPVSLTNLSGFEVVRVVSAEEMKEAVLSRSGEMEYIV
                     KAAAVGDYRPASYAGEKIKRSGSDTLRIDLIQNTDIAAALGERKRPGQVLIGFAAESH
                     DLQKNASEKIRKKNLDFIVANDITAPAAGFGADTNTVRILSADGSVSEFAGTKEDVAE
                     EIWNTVLADKQLS"
BASE COUNT          516 a          527 c          614 g          506 t
ORIGIN      
        1 atcatccttg agcagaggaa aaacagggag gcgcgaagat gaagttttat gatttggatt
       61 ctctcggaga aaagcaggga ctggacaaca agtatatcct cacggccgca gtagcggcaa
      121 gagcgcgctc gctcagcgaa cagaaaggtc gaacgcttga agaggacaac gagaagttca
      181 tctcgacagc tctgcgggat tttgatcttg gcctagtgcg cctttcatca gatgagcggg
      241 aaagcgaaga agctcccgtt gccgttgtct cagatgccca cgtggaagaa taaagtaaaa
      301 atcgtcctcg gcataacggg cggaatcgcc gcttacaaag ccccggagct tgtacgaacc
      361 tttgtgaagt ccgggtgcga ggtcgaagtt gtgttgaccg aggacggaga aaagttcgtc
      421 agtccgatgg tgctttcaac cctcgcggga agaagaatct ggcgtcagcg tgattttttg
      481 tcggatgaat acggatggag gattcctcac atttcgctgg cggattgggc ggacgttgtg
      541 gtggtcgctc cttgtacggc ggaaacgctt gcaaatacag ttcaaggaag aggtaaagag
      601 ctactcggtt ctcttcttct cgcgactcgg gcgcaggtgc ttctctgtcc ggcgatgaac
      661 gtgaatatgc tctccaatcc tgccacggcg gaaaacatcc gaactctaaa gaacaggggg
      721 tatgcggttg tcgatcccga ctccggagac ttggcgtgcg gttatgaagg gaaaggccgc
      781 cttccctccc cgtatgtact tcttgaggaa atgtggcgtg tagcctgtcc caagaaagag
      841 cttcaaagga aaaatgtcct tgtcactgcc ggaccaacct gggagttcct tgatccggtg
      901 cgttttctca gtaatccgag caccggaaag atgggggtcg ccgtggcccg ggctgcctgg
      961 tacagaggag cttccgtcac ccttgtgctt ggtccggtct ccttgaccaa tctttcggga
     1021 ttcgaggtag ttcgcgtcgt ttccgctgaa gagatgaaag aggctgtcct ctcacgctcc
     1081 ggggaaatgg agtacattgt aaaagcagct gcggtagggg actatcgccc cgcttcctat
     1141 gcaggagaaa agataaaacg aagcgggtcg gatacgcttc ggatagatct catccagaat
     1201 accgacatcg ccgccgccct cggtgaacga aaaagaccgg ggcaggtgct cattgggttt
     1261 gctgccgaaa gccatgacct tcagaaaaat gcctccgaaa agatacgaaa gaaaaattta
     1321 gacttcattg tcgcaaacga tataacagcc cctgcagcag gcttcggtgc agataccaac
     1381 actgtccgca tactgagtgc cgacggctct gtatcggagt ttgcagggac caaggaagac
     1441 gtagccgaag agatctggaa taccgttctc gcagacaaac agttatcgtg aagaagaggc
     1501 gcctccatgc catattatga cgttctcgtt ccaggcccat ggtggaacag tctgacgtat
     1561 acgacagagt tgaacgtacc gctcggcgca cgagtcgtcg ttcctttagg acgtggagag
     1621 cgtatcggtt ttgtgcagcg cctcagcaac gctgttcagg agggagacgg cgcgttctcg
     1681 attcggcgca taaagaggat tattgacgac gtttcttttt tcccaccgcc tttatgggag
     1741 ctttttgact ggggaggaag agtgtttctt tgcggaagtg gagaactgct tcgtatcgga
     1801 attccgtccg ctttcctttc accctcggat gccactcccg tacctcttcc attgcctcgg
     1861 atggagacca aggctcagga agagttgttt ttttatgcgt gtagtttcca agagcgttgt
     1921 aacaaattcc tctcccttct ggaaagaagc gagcgttttc ttgttctttt ccctgagcag
     1981 ggacttgcct ccgcgttttg gaagcagctt tccgagtctc aaaagaaaga gaccattctc
     2041 tggccgtccc agggagggaa gaaactcatt gcggcatgga ttcaagcgcg caataatata
     2101 ccgagaggaa tcatcggggg gcccggcgca gtttttgccc ctttacaaca gattgaaacc
     2161 att
//
LOCUS       DAOSWW010000452         2162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41080_length_2162_cov_3.776459, whole genome shotgun sequence.
ACCESSION   DAOSWW010000452 DAOSWW010000000
VERSION     DAOSWW010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41080_length_2162_cov_3.776459"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PK442_09085"
     CDS             complement(<1..1159)
                     /locus_tag="PK442_09085"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO87846.1"
                     /translation="MSKSVFYNGKIAVPGGFVEGMAIEGQNIVCLGSSEEVCAIRDAE
                     KIDLAGRLALPGFIDGHMHFLAYALSLERADLLGCFSVNEVRSRLRAFLDERRPEPGE
                     WVVGRGWNHELFDDNRILTKEDLDDLTPDNPAMLARVCGHIVVLNSRAITLLGLTGES
                     RFAGGVVDLDDAGEPTGIIRESVVEWAGSKLPLPGPEKLRKLVVRAGKETAKVGLTSI
                     HSDDLGSVGGDFQTIIDLYISLCKEDEMPVRITEQLLLRNRPALDYFLSTGWRTGDGM
                     PFFHIGPLKILTDGSMGGRTALLRKEYEDAPGVFGVPIYGHDELDDLVLTAHLAGMQV
                     ATHAIGDGALNMCLDAMERALLAKPRDARHYIVHCQMGDMEQYRRMARLGIG"
     gene            complement(1156..1422)
                     /locus_tag="PK442_09090"
     CDS             complement(1156..1422)
                     /locus_tag="PK442_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87847.1"
                     /translation="MRISGFSEDEDGNGCYLVEWSDTAGRKCAVLYSESGGSVESVSA
                     ERKRELFESGDLEACSFPASEVLFPDEVQKLAERFQIVVEEEKE"
     gene            1516..1962
                     /locus_tag="PK442_09095"
     CDS             1516..1962
                     /locus_tag="PK442_09095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOO87848.1"
                     /translation="MEQLPRSKGCFVCGNSDDNPRSLGLTIFWNDEEQRTEIPILPNS
                     TWCGYEGVVHGGIIASVFDDAMAWAIRRENGSWAVTGEMSVRYLRPVLAGTRYVVEGR
                     VEKRSGRRISTVAVMKEKNGKKVAEANALFVRIRERTISEGEEQTE"
BASE COUNT          502 a          631 c          568 g          461 t
ORIGIN      
        1 cgccgattcc aagacgcgcc atgcgccggt actgctccat atcccccatc tggcaatgca
       61 caatataatg acgcgcgtcg cggggttttg ccaggagcgc ccgttccatg gcgtcaaggc
      121 acatattgag cgcgccgtcg ccgatagcgt gcgtcgccac ctgcataccc gccagatgcg
      181 ccgtcagaac gagatcgtcc agttcgtcgt gcccgtagat agggacgccg aaaactcccg
      241 gagcgtcctc gtactctttt cgcagaagcg cggttctccc gcccatcgag ccgtcggtaa
      301 gaatcttcag cggcccgata tggaaaaaag gcatgccgtc gcctgtccgc cagcctgtcg
      361 aaaggaaata atcgagcgcc ggacggttcc ggagcagcag ttgctcggta atcctcaccg
      421 gcatttcatc ctctttacag agagagatgt agagatcgat aatcgtctga aaatcaccgc
      481 cgacgctgcc aagatcgtcc gaatgaatcg aggtaagccc gactttagca gtctcttttc
      541 ctgcacgcac tacaagtttc cgcagctttt caggccccgg aagagggagt tttgagcccg
      601 cccactccac gaccgattcg cgaatgatcc ccgtaggttc ccccgcatcg tcgaggtcaa
      661 caactcctcc ggcaaacctg ctctcgcccg taagcccaag aagcgttatc gccctggagt
      721 tgagcacaac gatatgaccg caaaccctgg caagcatagc ggggttatcg ggcgtcaggt
      781 cgtcaagatc ttcctttgta agaatccggt tgtcatcgaa aagttcatgg ttccagccgc
      841 gccctacaac ccattcgccg ggttccggcc ttctctcgtc gagaaaggct cgcagacgcg
      901 aacgcacttc attcacagaa aaacacccga gcaagtcggc tcgttcgaga gaaagcgcat
      961 atgcgaggaa gtgcatgtgt ccgtcgatga aaccggggag agcgagcctt ccggcaagat
     1021 caatcttttc ggcatcccgt atcgcgcaca cttcttccga acttccgaga caaacgatat
     1081 tctgtccttc tattgccatt ccctcgacga atcctcccgg aacggcaatc ttcccgttgt
     1141 agaacacgct tttgctcact ctttctcctc ctctacaaca atttgaaagc gctcggcaag
     1201 cttttgaact tcatccggga agagcacctc cgacgcaggg aaagagcagg cttcaagatc
     1261 gccgctttcg aagagctctc ttttccgctc ggcggagacg ctttcaacgc ttcctcccga
     1321 ttccgaatac agaacggcgc actttcgtcc cgccgtatca gaccactcga caagatagca
     1381 gccgttaccg tcttcgtcct cggaaaaacc tgaaatccgc atttggatcg acctccaaat
     1441 ttctttggct ccattatacc cttcttctct tcttcggcta taatcgtgcg aaagatttgt
     1501 gagaggagcg atacgatgga acaacttcca cggtcgaaag ggtgcttcgt ctgcgggaat
     1561 tcggacgaca accccaggag cctcggtctg accatattct ggaacgacga agaacagagg
     1621 acggaaatcc cgatcctccc gaacagtacg tggtgcggct atgaaggcgt cgtccacggg
     1681 ggaatcatcg cctccgtctt cgacgacgca atggcctggg cgatccgcag ggagaacggc
     1741 tcgtgggccg ttaccgggga gatgtcggtc cgctacctcc gccccgtcct tgcgggcacg
     1801 cgctacgtcg tcgaaggccg ggtggaaaag aggtcgggac gacgcatttc aaccgtcgcc
     1861 gtcatgaagg agaaaaacgg caagaaagtg gcggaggcga acgccctctt cgtgagaata
     1921 cgggagagga ccatttccga gggagaagag caaacggaat gaatgctcat cgtgattgcg
     1981 ccgctcgcgg cattacaaca gcgatatgat atactgtctt ttgtttgatt ttcgtgggaa
     2041 ggggcaatta ttgttcagaa atctctgtac gcgaaaaatc cggtctgaac agcttcaaat
     2101 tgagcgttct aaagatgccg gctatctcag taccgggaga ttatcgtaag ggagcgtggg
     2161 aa
//
LOCUS       DAOSWW010000453         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41156_length_2160_cov_2.916390, whole genome shotgun sequence.
ACCESSION   DAOSWW010000453 DAOSWW010000000
VERSION     DAOSWW010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41156_length_2160_cov_2.916390"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..819)
                     /locus_tag="PK442_09100"
     CDS             complement(43..819)
                     /locus_tag="PK442_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOO87849.1"
                     /translation="MGNNSVFAVFDLETNGFKGASTVSASSIVFTAEGRIVDFFNRFY
                     HSEERPDHRTERVHGLTPERIAHFREEERYPSYFLEDWPSLASFWERWNPEGIVVHNL
                     SFDLSFLPREAARGQKWWCSMRGLTDFCKIPKSADREGEWKWPRLGEAVRIARERLLE
                     PLPLAEAEEFVGPALGHYGLSDCFELYGLFVRVLNQAPERVRFRAASALQLSPRRELY
                     PLPSAVRDSFVRERLDYSARIAEAAGDAERSRKLRSLGTD"
     gene            941..1537
                     /locus_tag="PK442_09105"
     CDS             941..1537
                     /locus_tag="PK442_09105"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOO87850.1"
                     /translation="MNDAPFNALRNEVVACERCPLAATRLHAVFGEGPERTPVVLVGE
                     APGAKEDESGRPFVGLSGRLLSELLEEAGLPREKLFITNTVKCRPPENRLPSKAETAA
                     CRPFLAAQLRHIRPRLVITAGNAPTRALLGTKEGITTLRGRLHTCVWEGMELSVLPLF
                     HPSYLLRNRGRAEGSPIMLTLADIREALRLAREFTPGF"
     gene            complement(1521..>2160)
                     /locus_tag="PK442_09110"
     CDS             complement(1521..>2160)
                     /locus_tag="PK442_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOO87851.1"
                     /translation="VFFCGCQFRCPFCHNPELVFPGAEIERIGAEHILADLKKRAPFL
                     DGVCVTGGEPTLQPGLLPFLREIRSLGLKVKLDTNGARPDVLERILAEKLADYAAMDL
                     KAPEETYSLACGRDGDATLLPLVRKSIALLLRGAVPFEFRTTLVPGLHRIEDAPLLGR
                     MAQGAPLFVLQNFRPGNTLDPGFSSLSPFSMEFLEEFRTALLPFVGEVKIRA"
BASE COUNT          419 a          676 c          706 g          359 t
ORIGIN      
        1 ccgtagagaa tacgacgccc tgtcctctta tggagcggtc tctcagtcgg ttccaagcga
       61 gcggagtttt cggctccgtt cggcatctcc cgccgcttcg gcgatgcgcg ccgaatagtc
      121 gagacgctct ctgacgaacg agtctcgaac ggccgaaggc agagggtaga gctcacgccg
      181 gggggagagc tgcaacgcgg aggcagcgcg aaagcgcacc cgttccggcg cttggttcag
      241 gactctgacg aagagcccgt acagctcgaa gcagtcgctg aggccgtagt gtccgagggc
      301 cggtcccacg aattcctccg cctccgccag cggaagcggt tcgagcaggc gctcccgggc
      361 gatgcggacc gcctctccga ggcggggcca cttccattct ccctccctgt ccgcactctt
      421 gggaattttg cagaagtcgg tgaggcccct cattgaacac caccactttt gtccccgcgc
      481 agcctcgcgg gggaggaagg agagatcgaa cgagaggttg tggacgacga ttccctccgg
      541 gttccatcgc tcccagaagg aggcgaggct gggccagtcc tccaggaaat aggaggggta
      601 gcgttcttcc tcgcgaaagt gcgcgatccg ctccggcgtg agcccgtgga ctcgctcggt
      661 ccgatggtct ggacgctcct cggagtgata gaaacggttg aaaaagtcga cgatcctccc
      721 ctccgcggtg aagacgatcg acgaggcgga gacggtcgac gctcccttga agccgttcgt
      781 ctccaggtcg aagacggcga agacgctgtt gtttcccatg aaataccctc cctgcacacg
      841 tttcggaccc ctccggggcg ctcgcttttg aacgctccgg agacgctgct attatgacag
      901 aacagctccc gtaagggaaa gcgacgaaag gacgaaaaga atgaacgacg cgccattcaa
      961 cgcattgaga aacgaggtcg tagcctgcga acgctgtccc ctcgccgcga cgcggttgca
     1021 cgccgttttt ggggaaggcc cggaaagaac gccggtggtc cttgtggggg aggcccccgg
     1081 agcgaaggag gacgaatccg gacggccgtt cgtcggcctc tcgggacgtt tactgagcga
     1141 actgctggag gaggccgggc ttcccaggga gaagctgttc atcacgaaca ccgtgaagtg
     1201 ccgccctccc gaaaaccgcc ttccttcaaa agcggagacg gccgcctgcc gcccgtttct
     1261 cgctgcgcag ctgcggcaca tcaggccgcg cctcgtcatc accgcgggaa acgcgcccac
     1321 ccgcgcgctg ctggggacga aagaggggat aacgacgctg cgcggacgtc ttcacacctg
     1381 cgtctgggag ggtatggagc tctccgttct gccgctcttc cacccgagct acctccttcg
     1441 gaaccgggga cgcgccgaag gaagcccgat tatgctcacg ctggccgaca tccgtgaagc
     1501 gctccgcctg gcgcgggagt tcacgcccgg attttgacct ctccaacgaa ggggagaagc
     1561 gcggttcgga actcctccag aaactccatg gagaaggggg agagcgatga gaagccggga
     1621 tcgagcgtgt tccccggacg gaaattctga aggacgaaga gcggcgctcc ttgggccatg
     1681 cggccgagaa ggggcgcatc ctcgatacgg tggagccccg gaacgagcgt cgtccggaac
     1741 tcgaaaggga cggccccgcg cagcagaagg gcgatgctct tccgcacgag cggaaggagc
     1801 gtcgcatcgc cgtcccggcc gcaggccagg gaatacgttt cctccggagc cttgaggtcc
     1861 atcgccgcgt agtcggccag cttctccgcg aggatccgct cgaggacgtc cgggcgcgct
     1921 ccgttggtgt cgagcttcac cttgagcccg agcgatcgta tctccctcag aaaggggaga
     1981 agcccgggct gaagcgtcgg ctctccgccc gtgacgcaga cgccgtcgag gaagggggct
     2041 cgtttcttca agtctgcgag gatgtgctcc gcgccgattc gttcgatctc cgcgcccgga
     2101 aagacaagct ccgggttgtg gcagaaagga caccggaact gacagccgca aaagaagacc
//
LOCUS       DAOSWW010000454         2159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41175_length_2159_cov_4.369772, whole genome shotgun sequence.
ACCESSION   DAOSWW010000454 DAOSWW010000000
VERSION     DAOSWW010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41175_length_2159_cov_4.369772"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK442_09115"
     CDS             complement(<1..599)
                     /locus_tag="PK442_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO87852.1"
                     /translation="MPPSEPFPKGEKICVSQIQHFSVNDGEGIRSTVFLSGCPLRCRW
                     CCNPETWTSTPKSGRLPGAKEETFGRFLSVAEVMKELVRHAIFYRESGGGVTWSGGEP
                     FFFPGNLRALVRACADRGIPQAVETTCHFDWEECADIVGLLDFVFADIKHMDGGVHER
                     LTGIGNERILSNMKRLGATGVDAVVRVPLLSGVNDDEQNV"
     gene            complement(586..>2159)
                     /locus_tag="PK442_09120"
     CDS             complement(586..>2159)
                     /locus_tag="PK442_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOO87853.1"
                     /translation="QLGAILSENPLALNPGRFDQYMYPYYAADVEAGRLTPERALELV
                     ECLWIKFSEWVWTISSNTANYFAGYNQFQNLTVGGRKRDGSDGTNELSYICLKATEGV
                     KTHQPGLSVRISSDCPDDFLMAVSKLVATGMGFPAIHNDQAGAQMLLQAGYEPEDARD
                     WNNCGCVVPHFRKTGEWTSAVNVNFGAAMEYALNEGKSRLTGEPMGLPERAPEEFVSF
                     REVADAFLRQLANLVEHAVVGTLTAQKLHMEMVPRPFLSTCVDGCMQKGKDLSRGGAV
                     YNVGPVLTGIGLGVVANSLAAVKKLVFDEKVCTMKELCAALDADWEGYDELRERALAA
                     PKYGNDDDEVDSLAREISDFYYRTTRSHRDVFGSPFNSAFMGISNYVPTGKVIGALPC
                     GRKARAPLSEGVSPFAGTDVSTPLAAMRSAAKVNHDVQTGGTLLNLRLNKEIVSTPRG
                     LRNLGAMIRAYFSLGAFHVQFNTISTGELRAAQARPEEYRDLLVRVAGYSTQFVNLSR
                     EMQEAIIARTEHASF"
BASE COUNT          361 a          724 c          685 g          389 t
ORIGIN      
        1 acgttctgct cgtcgtcgtt gacgccggaa agcagcggca cgcggacgac ggcgtcgact
       61 ccggttgctc caagccgctt catattcgag aggatgcgct cgttgccgat tcctgtaagg
      121 cgctcgtgaa cgccgccgtc catgtgcttg atgtcggcga agacgaaatc gagcagccca
      181 acaatgtcgg cgcactcctc ccagtcgaag tggcaggtcg tttccacggc ctgcgggatt
      241 cccctgtccg cgcaggcccg gacaagcgcc cggaggttcc ccggaaagaa gaacggctcc
      301 ccgcccgacc aggtgacccc gccgcccgac tcccggtaga agatcgcgtg gcgtacaagc
      361 tccttcatca cctcggcgac gctgaggaat cgcccgaagg tctcctcctt cgcccccgga
      421 agacgcccgc tcttcggcgt cgacgtccat gtctccgggt tgcagcacca gcggcagcgg
      481 agagggcatc cggagaggaa gaccgtgctc cggatgccct ctccgtcgtt cacggagaag
      541 tgctgaatct gcgagacgca gattttctcc cctttcggga aaggctcaga aggaggcatg
      601 ttccgtcctg gcgatgatcg cctcctgcat ttcgcgggag aggttcacga actgcgtgct
      661 gtaccccgcc acgcgcacca gaaggtcccg gtactcctcc gggcgggcct gcgccgcgcg
      721 cagctccccg gtggagatgg tgttgaactg gacgtggaag gcccccagcg agaagtaggc
      781 gcgtatcatc gccccgaggt tccgcaggcc tctcggcgtg gagacgatct ccttgttcag
      841 ccgcaggttg agcagcgtcc cgccggtctg aacgtcgtgg ttcaccttgg ccgccgaacg
      901 catggccgcc agcggtgtgg agacgtccgt ccccgcgaac ggggagaccc cctcggagag
      961 cggcgcccgc gccttcctcc cgcagggaag cgcgccgatc actttcccgg tggggacgta
     1021 gttggagatt cccatgaagg cggaattgaa gggcgagccg aacacatccc tgtggcttct
     1081 ggtcgtcctg tagtagaagt cggagatctc ccgcgccagc gaatcgacct cgtcgtcgtc
     1141 gttgccgtac ttcggcgccg caagagcgcg ctcccgcagt tcgtcgtacc cctcccagtc
     1201 ggcgtcgagc gcggcgcaca gctccttcat ggtgcagacc ttctcgtcga agaccagctt
     1261 cttcaccgcg gcgagggagt tggcgaccac gccgaggccg ataccggtga ggacggggcc
     1321 gacgttgtac accgcgcccc ccctcgagag gtctttcccc ttctgcatac agccgtccac
     1381 gcaggtcgag aggaagggcc tggggaccat ctccatatgc agcttctgcg ccgtcagggt
     1441 gcccacgacg gcgtgctcca caaggttggc aagctgccgg aggaaggcgt ccgcgacctc
     1501 gcggaaggag acgaactcct cgggagctct ctcgggaagc cccatcggct ctccggtgag
     1561 gcggcttttt ccttcgttga gggcgtactc cattgccgcg ccgaagttca cgttcaccgc
     1621 cgaggtccac tcccccgtct tacggaagtg cgggaccacg cagccgcagt tgttccagtc
     1681 gcgggcgtcc tccggctcgt acccggcctg gagcagcatc tgcgccccgg cctggtcgtt
     1741 gtggatcgcg gggaaaccca ttcccgtggc gacgagcttc gagaccgcca tcaggaagtc
     1801 gtccgggcaa tccgaagaaa tacgaacgct caacccgggc tggtgcgtct tcactccctc
     1861 cgtggccttc agacagatgt aggagagctc gttcgtcccg tcgcttccgt cccgcttccg
     1921 cccgccgacg gtgagattct gaaactggtt gtacccggcg aagtagttcg ccgtgttgga
     1981 ggagatcgtc cagacccact ccgagaactt gatccagagg cactccacca gttcgagcgc
     2041 ccgctccggc gtcaggcgtc cggcctcgac gtcggccgcg tagtacgggt acatgtactg
     2101 gtcgaaccgg cccggattga gggccagcgg gttttcggag aggatcgcgc cgagctgga
//
LOCUS       DAOSWW010000455         2155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41308_length_2155_cov_3.151429, whole genome shotgun sequence.
ACCESSION   DAOSWW010000455 DAOSWW010000000
VERSION     DAOSWW010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41308_length_2155_cov_3.151429"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PK442_09125"
     CDS             <1..537
                     /locus_tag="PK442_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO87854.1"
                     /translation="LCIHPIQQRVRLFNSGREAGLHSFGESAKELLFYAALLHDIGLF
                     LSFSNHHAHSHYIIRNVELLGFHDNEIAVIAALAFYHRKRSPKKKDPEFEALDQDSQR
                     IVFTLSMFLRMAESLDRSHHNFVHEVRFVREKNVLLLKIQADKGYQLELWALEEHQKY
                     FVKTFGERFGIQVTASGF"
     gene            583..1260
                     /locus_tag="PK442_09130"
     CDS             583..1260
                     /locus_tag="PK442_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOO87855.1"
                     /translation="MKFRPEVKPFLLASFVFGVFIYTIAKVSGASNGLAFFLGLLFGG
                     AGTGYMLFFFRDPEIVTPAEPGIVVAAANGKLAKIVEMYEKDYLKQDTIRISIFLSLF
                     DVHVNRFPIGGFSTFLGYFPGKRLFTFDEKSSDVNQHNAILVRNEETSCLIRQIVGPV
                     CRRVVYWLREDKTTTVKQGDRFGMMKFGSRLDMYFPKDDIEMIAKIGDQVTAGETIIA
                     RIVKKGK"
     gene            1264..2061
                     /locus_tag="PK442_09135"
     CDS             1264..2061
                     /locus_tag="PK442_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOO87856.1"
                     /translation="MTGQQVHKLRILANWRRIVPTMVTLSALFFGFASILSTIEAMRL
                     DVPALLGRSAQYIMLALILDGLDGNVARWLKGTTEFGAELDTFVDFLAFGVAPAALLY
                     VMLIHGPSPFWRTILPSAIVFSGALRLSRFRLKDPLRGQGGFIGLPITVNSSWIALAA
                     FAAQIMYPEGHILVTGPVSIVFQFLILVMISLQLSNLRYPKPSNKVRFFAPAVLAVLL
                     LWALSETYAAWIATVLVFGCFVYVLTGPMIHKRSALRQQQQQHSEGK"
BASE COUNT          437 a          677 c          572 g          469 t
ORIGIN      
        1 ctctgcatac atcctatcca gcaacgtgta cgtctcttca acagcgggag agaggccggg
       61 ctgcactcct tcggcgagag tgcgaaggag ctgctcttct acgcggcgct gctccacgac
      121 atcgggctct tcctctcctt cagcaaccac cacgcccaca gtcactacat catccgcaac
      181 gtcgagcttc tgggcttcca cgacaacgag atcgccgtca tcgctgcgct cgccttctac
      241 cacagaaagc gctcgccgaa gaaaaaagac cccgagttcg aagccctcga ccaggattcg
      301 cagcgcatcg tcttcaccct cagcatgttc ctccggatgg ccgagagcct cgaccgaagc
      361 caccacaatt tcgtgcacga ggttcgcttt gtccgcgaaa aaaacgtgct tctgctcaaa
      421 atacaggccg acaaggggta tcagcttgaa ctctgggctc ttgaagaaca ccaaaagtat
      481 tttgtcaaga catttggaga acgtttcggt attcaggtga ccgcatccgg tttctagggt
      541 ataatgtaag gtgaatccat atacacacaa gggggctttt taatgaaatt taggccggaa
      601 gtgaaaccgt ttcttctggc gtcgttcgtg tttggcgtct tcatctacac gatagcgaag
      661 gtatcgggcg cttccaacgg actcgctttc ttcctcgggc tgctcttcgg cggcgcggga
      721 acggggtaca tgcttttctt cttccgcgat ccggagatag tcacaccggc ggaaccgggc
      781 atcgtggtcg ccgccgcgaa cggcaagctg gcgaagatcg ttgaaatgta cgagaaggac
      841 tatctgaaac aggacacgat ccgcatcagc atcttcctca gcctgttcga cgtccatgtg
      901 aaccgcttcc cgataggggg cttctcgacc ttccttggct acttccctgg gaaacggctc
      961 ttcaccttcg acgagaagtc gtcggacgtg aaccagcaca acgccattct ggtacgcaac
     1021 gaagaaacct cgtgtctcat acggcaaatc gtcggccccg tctgccgccg cgtcgtctac
     1081 tggctcaggg aggacaagac gaccaccgta aagcagggcg accgtttcgg catgatgaag
     1141 ttcggctcgc gccttgatat gtacttcccc aaggacgaca tcgagatgat cgcgaagatc
     1201 ggcgaccagg tcaccgcggg cgagacgatc atcgcccgga tagtgaagaa aggaaagtaa
     1261 acaatgacag gacagcaagt gcataaattg agaattctcg ccaactggag acggatcgtc
     1321 ccgaccatgg tgacgctctc cgcgctcttc ttcggcttcg ccagcatact ctccaccatc
     1381 gaggcgatgc gcctcgatgt tcccgcgctg cttgggcgtt ccgcgcagta catcatgctc
     1441 gcgctcattc tcgacgggct cgacggcaac gtcgcccgct ggctgaaagg aacgacggag
     1501 ttcggcgctg agctggatac cttcgttgac ttcctcgcct tcggcgtagc tccggcagcg
     1561 ctgctctacg tcatgctcat tcacggcccc agcccgttct ggcgcaccat tcttccctct
     1621 gccatcgtct tctccggcgc gctccgcctt tcgcgcttcc ggctgaagga tcctctgcgc
     1681 ggccagggcg gcttcatcgg ccttcccatc acggtgaact cttcgtggat cgcgctcgcg
     1741 gccttcgccg cgcagattat gtaccccgag gggcacatcc tcgtcaccgg cccggtctcc
     1801 atcgtcttcc agttcctcat cctggtgatg atctcgctgc agctctccaa cctgcgctac
     1861 cccaagccgt ccaataaggt gcgcttcttc gccccggcgg ttctcgccgt tcttcttctg
     1921 tgggcgctca gcgagacgta tgccgcgtgg attgccaccg ttctggtctt tggatgcttc
     1981 gtctacgtgc tcacaggccc gatgatccac aagcgttcgg ctctgcgtca gcagcaacag
     2041 cagcatagcg aaggaaaata agatcatgcg ggccatcggc gtcgatctgg gaggacacaa
     2101 aatagccgca gctctcgtcg aagacggacg gatcgttgaa cgtctctccg aaccg
//
LOCUS       DAOSWW010000456         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41366_length_2153_cov_3.816969, whole genome shotgun sequence.
ACCESSION   DAOSWW010000456 DAOSWW010000000
VERSION     DAOSWW010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41366_length_2153_cov_3.816969"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1519
                     /locus_tag="PK442_09140"
     CDS             338..1519
                     /locus_tag="PK442_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOO87857.1"
                     /translation="MKKRLMRDGIWMMGAVDWNRRLFDSLIPLPEGTSYNAYLVKGSE
                     KVALIDTADPELEDVLMEQLEGVERIDYIISLHTEQDHSGAIPTVLAKYPEARVVCST
                     KAKELLIDHIHLDESVIDAMPDGSTLSLGDRHFTFIHTPWVHWPETMCAYLHEERILF
                     SCDFFGSHLATSDMYAGESPLIYEGAKRYFAEIMMPFRSIVKSNIKKVEGYAIDIIAP
                     SHGPAFDKPEFIMNAYRDWTSDRVSNFVAIPYISMHGSTEIMVNRLLDALVERGVKVQ
                     KYELTVTDIGKLAIDLVDAATIVIGTPTVHFGPHPAVFSATHLANALRPKVKYAAIIG
                     SYGWGTKAVEQISGLIPNLKVEVLGTVMCKGLPREEDLKALDELADAIAAKHAPLMQA
                     E"
     gene            1699..2046
                     /locus_tag="PK442_09145"
     CDS             1699..2046
                     /locus_tag="PK442_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87858.1"
                     /translation="MKRIWCAAAFLFVLLAASCAFAGAQDFVLVNKTGVEIYAVYIAP
                     SDSEDWEEDVLAVETLPSGNSVKIRFAPGADVEYWDIRVEDSDGNALEWYEFNLLEIS
                     KITLLKNGEAQYE"
BASE COUNT          461 a          625 c          642 g          425 t
ORIGIN      
        1 gtccgcaatg ggaatgcagc acatggtgat cgacgcgatg aaggcgggag cgaccgattt
       61 cgtcgtcaag ccgttcaagc cggaacagct cctcgctgcg ctggagggga aaccggcgga
      121 ataggcctcc ccggcctgcc ggcgctaagg agcgcgcagg aatgttgttg tgtttttcct
      181 ccggactcgg agctgtcctg tccggaggaa aaagtgcgcg tattcttgcc cttaaaaagt
      241 tttattgata taatttatac gcttgaggag tatttcacga ccggacacgc gatcggtcgt
      301 agagatttta cagcgaagga cgaaaggaga tacagcaatg aaaaagagac tcatgcgcga
      361 cggcatctgg atgatgggcg cggtggactg gaaccgccgc ctcttcgatt cgctcattcc
      421 gcttcccgag gggacgagct acaacgcgta cctggtgaag ggcagcgaga aggtcgctct
      481 catcgacaca gccgatcccg aactcgaaga tgtcctgatg gagcagctcg agggcgtgga
      541 gcggatcgac tacatcatct cgctgcacac cgagcaggac cactccgggg ccatcccgac
      601 ggtgctcgcc aagtacccgg aggcccgggt ggtctgctcc acaaaggcga aggagctgct
      661 gatcgaccat attcacctgg atgaatcggt gatcgacgcg atgcccgacg gctccactct
      721 ctccctcggc gacaggcact tcaccttcat ccatacgccg tgggtccact ggccggagac
      781 gatgtgcgcc tacctgcacg aggagcgcat cctcttctcg tgcgacttct tcggttcgca
      841 cctcgccacg tcggacatgt acgccggtga gagcccgctg atctacgagg gggcgaagcg
      901 ctacttcgcc gagatcatga tgcccttccg ctccatcgtg aagagcaaca tcaagaaggt
      961 ggaggggtac gcaatcgaca tcatcgcccc cagccacggc ccggccttcg acaagccgga
     1021 gtttatcatg aacgcctacc gcgactggac ctccgacagg gtgtccaact ttgtcgccat
     1081 cccctacatc tcgatgcacg ggagcaccga gatcatggtg aaccgcctcc tcgacgctct
     1141 tgtggagcgc ggcgtcaagg tgcagaagta cgagctcacc gtcaccgaca tcggcaagct
     1201 ggccatcgac cttgtggatg ccgcgaccat cgtcatcggc acgccgaccg tccactttgg
     1261 tccgcacccc gcggtcttct ccgcgacgca cctggcgaac gcgctgcgcc cgaaggtgaa
     1321 gtacgcggcc atcatcggct cgtacggatg gggcaccaag gcggtggaac agatctccgg
     1381 cctgatcccc aatctgaagg tcgaggtgct gggaaccgtg atgtgcaagg gacttccgcg
     1441 cgaggaggac ctgaaggcgc tcgacgagct ggccgacgcc atcgccgcga agcacgcccc
     1501 gctgatgcag gcggagtaga cggcagtttt ttccgaggaa ctgaccggag gagaggcccg
     1561 tttgcggaaa tttctcctcc gctatattga acgctgaaaa aaacaggtag tgcgcgccgt
     1621 tgtcttgtgt ttttgaagcc gtctgctatc attcgcagga aggcgcttat ctcgtaaaaa
     1681 gaggggcagg ttgtcacaat gaaaagaata tggtgtgcag cagcgttttt gtttgtgctt
     1741 ctggcggctt cgtgcgcttt tgccggagcg caggactttg tgcttgtgaa caagacggga
     1801 gtggagatct acgccgtcta tatcgctcct tccgacagcg aagactggga agaggatgtg
     1861 ctcgccgtgg aaaccctgcc cagcggaaac tccgtcaaaa ttcgcttcgc ccccggagcg
     1921 gacgtcgaat actgggacat ccgcgtggag gacagcgacg gcaacgccct cgagtggtac
     1981 gagttcaatc tgcttgaaat atccaagatt accctcctga aaaacggcga agcacagtac
     2041 gagtaagtac gcatcgtcat ctgcgcatac acaagaggcc gttcccgaat ccgggggcgg
     2101 cctcttgtga tgcgtacgcc gttatttttt gaaaaccttc agcccggcgc ttt
//
LOCUS       DAOSWW010000457         2152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41412_length_2152_cov_3.112542, whole genome shotgun sequence.
ACCESSION   DAOSWW010000457 DAOSWW010000000
VERSION     DAOSWW010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41412_length_2152_cov_3.112542"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PK442_09150"
     CDS             <1..303
                     /locus_tag="PK442_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87859.1"
                     /translation="SSPRDTGLMKWAALADRGARKDFLDLYELSLQGVPLEDLFYLLP
                     RKFPGSRLNLYHMVKSLSFFDDAEGDPWPVMLRKVEWSVLKRHFLNVQRKLLGILG"
     gene            complement(378..>2152)
                     /locus_tag="PK442_09155"
     CDS             complement(378..>2152)
                     /locus_tag="PK442_09155"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOO87860.1"
                     /translation="ADRETRFNIGSTSKMFGATAILLLADEGKLSLDDPVAKHLPEFV
                     MRDGRYREITVRMLFNHSSGLPGSSFIFEYEPAYDPHALLLDVLRESDLKHAPGAMGI
                     YCNDGFTLAEMVVEKLSGKKFIDFVAERIFEPLGMKDSAASVGETGGNVAVYYDVPTG
                     KKYPLEVVRVHAAGGLSSTAEDLCRFADSFTPGGKHILSKASLEEALKSQPTLFTKHL
                     HDAAMMDAFGWDYAWIPDYREKGVQVLAKSGGTMFYSTNFQVVPAERLVVAVSIAGRA
                     DAPGVSRAILDALMKEKGLPFPGKPPLKKPGEPQPIPSELLAFEGAYSDGERAVRFLF
                     DPEKRTLNIHHLLPAPPQGEEAPPPVSLVYSDGVFHNYEKGTSYYFASEGKDVYLIME
                     KIPGFGMNVPAFQRLEEIKEPKSLSIEMNGVLWMARNILPGAQIMNGTLMGWSSTSHD
                     LPGYVFGFSLQKVEDFSFASIASTAFRDQFALQLSRKDGKIRARTGIFVLSPADEAET
                     LADGKNSVTIGADAENEWRKAGKDMILSFVRPEKGRVIAVDPENDAALLYDSVVDSGE
                     FFAPEGTFLFLAGAAGDVFEIEAR"
BASE COUNT          400 a          684 c          643 g          425 t
ORIGIN      
        1 tcctccccgc gcgacaccgg cctgatgaag tgggccgcgc tggccgaccg gggagcgcgc
       61 aaagattttc tcgacctcta cgagctctcg ttgcagggcg tcccgctcga agacctcttc
      121 tatctgctcc cgcgcaagtt tccgggcagc cgcctcaacc tctatcacat ggtgaagagc
      181 ctcagcttct tcgacgacgc cgagggcgac ccgtggccgg tgatgctgcg gaaggtcgag
      241 tggagcgtgc tgaagcgcca cttcctgaac gttcagagga agctgctggg aattctggga
      301 taacgaaaag aggaggggaa gcgcagattg tcgcgccccc tcctctttgt ctgtccggct
      361 gtaaaaaatg gacggctcta tctggcctct atctcgaaca catcccccgc cgcgcccgcg
      421 aggaagagaa aggttccctc cggcgcgaaa aactccccgc tgtccacaac gctgtcgtaa
      481 agcagcgccg cgtcgttctc ggggtcgacc gcgatcaccc gtcccttttc gggccggacg
      541 aaggagagga tcatgtcctt gccggccttc cgccactcgt tttcggcgtc ggcccctatc
      601 gtcacgctgt tttttccgtc ggcgagcgtt tccgcctcgt cagccggaga gagaacgaat
      661 attccggtcc ttgcccttat tttgccgtcc ttccgggaga gctgaagagc gaactggtcg
      721 cggaaagccg ttgacgctat gctcgcgaag ctgaaatcct cgaccttctg cagagagaaa
      781 ccgaagacgt acccgggcag atcatggctc gtggacgacc agcccatcag cgtcccgttc
      841 ataatctgcg ccccgggaag tatgttccgc gccatccaga gcaccccgtt catctcgatg
      901 gaaagagatt tcggctcctt gatctcctcc agcctctgga acgccgggac gttcatgccg
      961 aagccgggaa tcttctccat gatcaggtag acgtcctttc cctcggacgc gaaatagtag
     1021 gaggttccct tctcatagtt atgaaacact ccgtcgctgt agaccaaaga gacgggagga
     1081 ggcgcttcct ctccctgcgg aggcgcggga agcaggtggt ggatattcag cgtccgcttc
     1141 tccgggtcga agaggaaacg gaccgccctc tcgccgtcgg agtacgctcc ctcgaaggcc
     1201 aagagctcgg aggggatagg ctgcggctcc ccgggttttt tcagaggcgg cttcccgggg
     1261 aaggggagcc ccttctcctt catgagcgcg tcgagtatcg cacggctaac gccgggagcg
     1321 tccgctctgc ccgcgatact cacagcgaca accaggcgct cggcaggaac aacctggaag
     1381 ttggtggagt agaacatcgt cccgccgctc tttgcgagca cctgcacacc cttctcgcga
     1441 tagtccggaa tccacgcgta gtcccacccg aaggcgtcca tcattgcggc gtcgtgcaga
     1501 tgcttcgtga aaagtgtcgg ctggctcttc agcgcctcct caagcgatgc cttgctcaga
     1561 atatgttttc ccccgggcgt gaagctgtcc gcgaaacggc acaggtcctc ggccgtggag
     1621 gagagtcctc ccgccgcatg cacgcggacg acctcaagag ggtatttttt gccggtcggg
     1681 acgtcgtagt acacggcgac gttgccgccg gtctcgccga cgctcgcggc gctgtccttc
     1741 attcccagcg gctcgaagat gcgctccgcg acgaagtcga tgaacttctt ccccgagagc
     1801 ttctccacga ccatctccgc gagggtgaag ccgtcgttgc agtagattcc catggccccc
     1861 ggcgcgtgct tcagatccga ctcgcgcagg acgtcgagca gcagggcatg cggatcgtac
     1921 gcgggctcgt actcgaaaat aaagctggaa ccgggaaggc ccgaggagtg gttgaagagc
     1981 atccgcaccg tgatctccct gtaccggccg tcgcgcataa cgaactcggg caggtgcttc
     2041 gccacgggat cgtcgaggga gagtttcccc tcgtcggcca gcagcaggat cgccgtcgcg
     2101 ccgaacatct tgctggtaga gccaatgttg aagcgcgtct ctctgtccgc gg
//
LOCUS       DAOSWW010000458         2149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41493_length_2149_cov_4.150430, whole genome shotgun sequence.
ACCESSION   DAOSWW010000458 DAOSWW010000000
VERSION     DAOSWW010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41493_length_2149_cov_4.150430"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK442_09160"
     CDS             <1..313
                     /locus_tag="PK442_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOO87861.1"
                     /translation="CELPSYVIEVTESVQKAALNGASIISLTDSPAAPVCQSSTLSFF
                     VSDTTPTFGSSLVGPAFLVHILTSVLAVNLGESARVALQDQEMGLHDERIYHPAYGLR
                     Y"
     gene            complement(440..1126)
                     /locus_tag="PK442_09165"
     CDS             complement(440..1126)
                     /locus_tag="PK442_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOO87862.1"
                     /translation="MATLRYLGHSAFEIRGKDTVLLIDPFLSGNPAGAAATESEFTDV
                     RYIFVTHGHGDHLGDTVSIASRTGATVVTNYEICHYLGKKGVTCHAMHIGGTFSFPFG
                     RVKMTPALHGSDIMEGDRMIPGGNPCGFLFDVDGDRIYHAGDTGLTMDMQLLAVEKVK
                     AALIPIGGNFVMDVYDAARAVEFVRPEIAVPIHYDTFPVIAADPREFERLVGANATVK
                     IMKTGDTLEL"
     gene            complement(1187..>2149)
                     /locus_tag="PK442_09170"
     CDS             complement(1187..>2149)
                     /locus_tag="PK442_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HOO87863.1"
                     /translation="FGSDKPDLRIPFEIVDLSPVFAGSSFEPFRKIAAQGGYVRGIPL
                     PGGASLSRKQLSDIEEKAKRLGARGLSPFQIKDGELKGPLVKYLDESSRSLLKSSSSI
                     DDGDALFVVAGSDRREISEILGALRLELAREHGLVAKGAWEFLWVVDFPLFERDEEAG
                     RWSAVHHPFTSPCSEDIPLMDSNPGEVRSRAYDCVLNGNELGGGSIRIHDPLLQEKVF
                     SCLSFTKEAARERFGFLLDALSSGTPPHGGIALGMDRLAMLLSGAASIRDVMAFPKTQ
                     KAQCLMSGAPSPVPEEQLKELCIKTAPSAPSEAPAPAQEKMDLK"
BASE COUNT          471 a          649 c          568 g          461 t
ORIGIN      
        1 ctgcgaactg ccgagctacg tcatcgaagt cacagagtcc gttcaaaagg cggcgttgaa
       61 cggtgcttca atcatatcgc ttacagacag tcccgccgcc cctgtctgcc agtcgtcgac
      121 gctctccttc ttcgtgagcg atacgacgcc aaccttcggc agcagtctgg tcggtccggc
      181 cttcctcgta cacatcctta cttctgttct tgccgtcaac ctgggcgaat ccgcccgtgt
      241 agcgctgcaa gatcaggaga tgggacttca cgacgaacgc atttaccacc ccgcatacgg
      301 attgagatac tgaaaccccc tgcgccctgc atacttccgg tcctctttga gcaggaacac
      361 tatcataatt cctgtctaaa gaagaccggc ggtaattcat tattctcttt gaccgcgttg
      421 catccgttta cccttcttat cacagctcca gcgtatcccc ggttttcatg atcttcacag
      481 tcgcgttcgc accgacaagg cgctcaaact cacgcggatc cgccgcgatc accggaaaag
      541 tgtcatagtg tatcggcaca gctatttcag ggcggacaaa ctccacggca cgggcggcgt
      601 cgtacacgtc catcacaaag ttcccgccga tggggatcag cgccgccttt accttttcta
      661 cagccagaag ctgcatatcc atcgtaagac cggtgtctcc tgcatgatag atccgatctc
      721 cgtccacgtc aaagagaaaa ccgcagggat ttcccccagg tatcatccgg tccccctcca
      781 tgatgtccga cccgtggagc gcaggagtca tcttgacgcg tccgaagggg aaggagaatg
      841 tgccaccgat atgcattgcg tggcacgtaa cgcccttttt tccgaggtaa tgacatattt
      901 cgtaattcgt tacaaccgtg gcgcctgtcc tggaagcgat ggagacagta tcgcccagat
      961 gatctccgtg tccgtgggta acgaagatat agcgtacatc cgtgaactca ctctcggtcg
     1021 ccgcggcgcc cgcaggattt cccgaaagaa acggatctat cagaagcacc gtatccttcc
     1081 cccgaatctc gaatgccgaa tgtccgagat accgtaatgt cgccaaatat atctcctcct
     1141 ttatgcacgg gcccccgctg tgaacacggg ggcccggatt tcgtgatcac ttcaggtcca
     1201 ttttctcctg cgccggagct ggcgcttcag acggcgccga cggagctgtc tttatgcaga
     1261 gttctttcaa ctgctcctcc ggtactggcg acggcgcgcc agacataaga cattgagcct
     1321 tttgcgtctt tgggaacgcc atcacgtcgc gaatggatgc agcgccgctc aggagcatcg
     1381 ccagcctgtc cattccaaga gcgatacctc cgtggggagg agtgccggaa gaaagcgcgt
     1441 cgagcaggaa tccgaaacgc tctcgcgccg cttcttttgt aaaggagaga caggagaaaa
     1501 ccttttcctg cagaagcggg tcatggattc tgatggaacc gccgccaagc tcgttgccgt
     1561 tgaggacgca gtcgtacgcc ctggaccgca cctcgccggg atttgaatcc atgagcggaa
     1621 tatcttcaga gcagggagag gtgaaggggt gatgcaccgc gctccagcgt cccgcctcct
     1681 cgtcgcgttc gaacaggggg aaatccacaa cccagagaaa ctcccaggcg cctttcgcga
     1741 caagcccatg ctcgcgagcc agttcaaggc gaagcgcccc gagaatttcg gaaatctcgc
     1801 gacggtcgct ccccgcgacc acaaagagcg cgtcgccgtc gtcaatggac gaggatgatt
     1861 tgagcaacga tcgggagctc tcgtcaaggt attttaccaa aggtcccttc agctctccgt
     1921 ctttgatctg gaagggagaa agaccgcggg cgccgaggcg cttcgctttt tcctcgatat
     1981 cggaaagttg ctttcttgag agcgaggccc ctccgggaag aggaataccg cggacatacc
     2041 ctccttgcgc cgcgattttg cggaacggtt caaaggagct ccctgcaaat accggggaga
     2101 gatcgacaat ttcaaacggg atgcggagat ccggcttatc gctcccgaa
//
LOCUS       DAOSWW010000459         2147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41574_length_2147_cov_3.394359, whole genome shotgun sequence.
ACCESSION   DAOSWW010000459 DAOSWW010000000
VERSION     DAOSWW010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41574_length_2147_cov_3.394359"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PK442_09175"
     CDS             <1..820
                     /locus_tag="PK442_09175"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOO87864.1"
                     /translation="RVMDTPISETAIVGACVGAALAGMRPVADMHFADFAGVCFDEIY
                     NQMAKIYYMFGAQKPVSMVLRAPDGLVNQAAAQHSQSLEAFFMHIPGLKVVIPSNPAD
                     AKGLLKAAIREDNPIIYFEHKALFSMKGEVPEEEYLTPIGKAKVVKEGSDVTLVSYSM
                     TMNLCVKAAELLAKDGISAELIDLRTISPLDKETILESVAKTTRLAIAHEAVKQGGVG
                     AEIAALVAEEAIDCLDAPIVRVGAPFTPVPFARPLEQAYRITPEKIAAAVRAMF"
     gene            830..1321
                     /locus_tag="PK442_09180"
     CDS             830..1321
                     /locus_tag="PK442_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOO87865.1"
                     /translation="METERVSSAAAEIGRTILVRLAPGEDVFEGLRTVCERHGLRSGR
                     IATMIGSLRTASLVCVTPDPDAPGRVVYLEPLTIEGPLELIAVQGVFGWDDDKDELSI
                     HLHAQVAGEDLRPFAGHLADTGKNRILATAEIVLDVFEGAELRRSFDEETGFTLFKIF
                     PKL"
     gene            1457..>2147
                     /gene="dctP"
                     /locus_tag="PK442_09185"
     CDS             1457..>2147
                     /gene="dctP"
                     /locus_tag="PK442_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO87866.1"
                     /translation="MKKLVALLILVTALCAFSVPAFAAYKAEYKLDIVPSLTTSWGMG
                     AVYFTELVKERSGGKINIKVYPGSQLTVGAQTNAFMLVRNGTIDFAVQSSINYSPQII
                     ELNLFALPFFIANQPDKYAALDAVKAGKSGEMVVKAVEDKGVKFLAWGENGFREMTNS
                     KKEIRTPQDMEGMKIRVVGSPIFLDAFKAIGANPITMNWSDTMAAIQQGVVDGQENPI
                     STFYPVKMHEYH"
BASE COUNT          458 a          611 c          673 g          405 t
ORIGIN      
        1 acgggtgatg gatacgccta tcagcgagac ggcgatagtg ggcgcctgcg tgggcgcggc
       61 gcttgccggg atgcgtccgg tcgcggacat gcactttgcg gatttcgccg gggtctgctt
      121 cgacgagatc tacaaccaga tggcgaaaat ctactacatg ttcggcgcgc agaagcccgt
      181 ctccatggta ttgcgcgcgc cggacggcct tgtgaatcag gctgcggcgc agcactcgca
      241 gtctcttgag gcgttcttca tgcacatccc gggactgaag gtcgtcatcc cctcgaaccc
      301 ggcggatgcg aaggggttgc tgaaggcggc gatccgcgag gacaacccga tcatctactt
      361 cgagcacaag gcgctcttct ccatgaaggg agaggttccg gaggaagagt acctgacgcc
      421 catcggcaag gcgaaggtcg taaaagaggg aagcgacgtg acgctcgttt cgtactcgat
      481 gaccatgaat ctctgcgtca aagcggcgga gttgctggcg aaggacggca tcagcgccga
      541 gctgatcgac ctccggacga tttcgcctct ggataaggag acgatcctcg agtcggtggc
      601 gaagaccacc cgcctcgcca tcgcgcatga agctgtcaag cagggcggcg tgggcgcgga
      661 gatcgcggct ctcgtcgccg aggaggcgat cgactgcctt gacgccccca tcgtccgcgt
      721 cggcgctccg ttcaccccgg ttcccttcgc gcgcccgctg gagcaggcgt accggattac
      781 tccggagaag atcgccgccg ccgtcagggc gatgttctag gaagaggcca tggagacgga
      841 acgggtttcg tcggcggctg cggaaattgg acggacgatt ctcgttcgtc tcgctccggg
      901 agaggacgtc ttcgaggggc tgcgcaccgt atgcgaacgc catggactcc ggtccgggcg
      961 aatcgcaacg atgataggga gtctgcgcac cgcgagcctc gtctgcgtga cgcccgatcc
     1021 ggacgctccg gggcgggtgg tctatctcga acccctgact atcgagggcc cgcttgaact
     1081 gatcgcagta cagggagttt tcggctggga cgatgacaaa gatgaacttt cgatccatct
     1141 gcacgcgcag gtggccgggg aggatcttcg tccgttcgcg gggcatctgg ccgatacggg
     1201 gaagaaccgg attctcgcca cggcggagat agttctcgac gtttttgaag gcgccgaact
     1261 caggcgctcg ttcgacgagg agacagggtt cacgcttttc aagatttttc cgaaactgta
     1321 atcatacgtt acaatacaag gacaatgtcg ctggaacgag gaggtgatcg ctccgctacc
     1381 gaaggaagtg tggaaggaaa ggcgctattc cgaacatggg aagttgtaaa accacattca
     1441 ggaggtaaag aagaagatga aaaaactcgt tgcgctgctc atacttgtaa cagctctgtg
     1501 cgcgttctcc gttccggcct ttgcggccta caaagccgag tacaaactcg atatcgtccc
     1561 cagcctcacc acatcctggg gtatgggcgc cgtctatttc accgaacttg tgaaggagag
     1621 aagcggcggc aagatcaaca tcaaagtgta tcccggctcc cagcttaccg taggagcgca
     1681 gacgaacgcg ttcatgctcg tccggaacgg gacgatcgac ttcgcggtgc agtcctccat
     1741 caactactct ccgcagatca tcgagctgaa cctcttcgcc cttccgttct tcatcgcgaa
     1801 tcagcccgac aagtacgccg cgcttgacgc ggtcaaggcg gggaagtccg gagagatggt
     1861 cgtcaaggcc gtcgaggaca agggtgtgaa gttcctcgca tggggagaga acggcttccg
     1921 ggagatgaca aactccaaga aggagattcg cactcctcag gatatggaag ggatgaagat
     1981 ccgcgtggtc ggcagcccga tcttcctcga cgccttcaag gcgatcggcg ccaaccccat
     2041 caccatgaac tggagcgata ccatggcggc catccagcag ggcgttgtgg acggccagga
     2101 gaaccccatc agcacgttct atcccgtgaa gatgcatgag taccaca
//
LOCUS       DAOSWW010000460         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41598_length_2146_cov_4.313725, whole genome shotgun sequence.
ACCESSION   DAOSWW010000460 DAOSWW010000000
VERSION     DAOSWW010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41598_length_2146_cov_4.313725"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2146
                     /locus_tag="PK442_09190"
     CDS             <1..>2146
                     /locus_tag="PK442_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLAT/LH2 domain-containing protein"
                     /protein_id="HOO87867.1"
                     /translation="SMRAGETRSGAAEVDMLYEGMESEPSVWGYSVEGRGSLSVTAGR
                     GGPVSGEIAHSFFVPKMYGFRLELFDPELNRRAPLEYHLRGLRVFDEEGNDITSSLTY
                     SKRSTFDLFTGVAAGLPGKPVRLRVRVHEAEAVVDLQFADNTHPKETHPWNIPSPVAR
                     ITLGPNKSPVADFRIRPQDPKWSPETILDASPSYDPDGIITRYSWTLEAGSPPDARND
                     SVIAHRFPAGGVQTVTLSLTDNKGATSTVTKRVHTSKPFTVRGDEFAAQSVFAVPPTA
                     SYEVTVRTGDRVKSGSDAGVFLALYGPADEQGTPTGSGEIHLLPSSSLQCTSTDPFER
                     GGTDVFTIPEIYGFGSLDEAERMTLRHDNGGDGAGWFVDGVQVKNLKNGKSWTFPANR
                     WLATDEGDGKTYAEFTPITGAYERGILFGGDARSIGMIEASDRIFILPAGASSFYFTQ
                     LEGSRELRVYRNDVLVGTHSQAGRGLRDPLFLPESGWGVSYDASLITAPARFRVQTLD
                     SGSVREDGSVWVLPQSWAGHEKTVRQAVMADALIRELRSGLSGEDPYGVFACGKSAVK
                     VFAAARPNLGVAGVKILRYGMSSLSIFGDFDDVQFDLFTELASNAYFSSKLPALVAQL
                     LTGTFGSVTTEMLSLLKSIFNAAEWGAEFFSTSIFDAPSDVYAVDLLNDIAVSDSFFL
                     QSAELLQASGEKLRAAVSAFEQNDPEACAAAFG"
BASE COUNT          369 a          636 c          728 g          413 t
ORIGIN      
        1 agcatgcgcg ccggggagac gcgcagcggc gccgccgagg tggacatgct gtatgaaggg
       61 atggagagcg agccctcggt gtgggggtac tccgtcgagg ggcgggggag cctctcggta
      121 accgccggac gcggcggccc cgtttccgga gagatagcgc actccttttt cgtcccgaag
      181 atgtacggct tccgcctgga gctcttcgat ccggagctga acaggagagc gcctctcgag
      241 taccatctgc gcgggcttcg ggtgttcgac gaggagggga acgatattac ctcctcgctg
      301 acctatagca agaggagcac gttcgacctc ttcaccggcg tcgcggcggg actgccgggg
      361 aagcccgtca ggctgcgcgt gcgcgtgcac gaggccgagg cggtggtgga cttgcagttc
      421 gccgataata cgcatccgaa ggagacgcat ccgtggaata tcccttcgcc cgtggcgcgg
      481 atcacgctcg gacccaacaa gtctccggtg gccgatttcc gcatccggcc gcaggatccg
      541 aagtggtcgc ccgagaccat tctggacgcc tcgccgtcgt acgatccgga cgggatcatc
      601 acccggtact cctggacgct ggaggcgggg agtccccccg atgcccggaa cgattccgtc
      661 attgcccacc gcttcccggc gggaggcgta cagacggtga cgctgagtct gaccgacaac
      721 aagggggcga catccactgt cacaaaaaga gtacacacct caaagccctt tacggtgcgg
      781 ggggatgagt tcgccgctca gagcgtcttt gccgtgcccc cgacagcgtc gtacgaggtg
      841 acggtgagga ccggcgatcg tgtgaagagc ggctccgacg ccggggtctt ccttgccctc
      901 tacggtcctg ccgacgaaca gggcacgccg acgggatcgg gggagattca tctgctcccg
      961 agttcgagcc ttcagtgtac ctccaccgac ccgttcgaac gaggagggac ggatgtcttc
     1021 accattccgg agatctatgg attcggttcg ctcgacgagg cggagcggat gacgcttcgt
     1081 catgacaacg gcggtgatgg agccgggtgg ttcgtggacg gcgttcaggt caagaatctg
     1141 aagaacggga agagctggac cttcccggcg aaccgctggc ttgcgacgga cgagggggac
     1201 ggcaagacgt acgcagagtt caccccgatt acgggcgcct acgagcgcgg aattctcttc
     1261 gggggagacg cccgctccat cggcatgatc gaggcgagcg acaggatctt catcctgccc
     1321 gcaggcgctt cgtccttcta cttcacgcag cttgaaggat cgcgggagct ccgcgtctac
     1381 cggaacgatg tgctggtagg gacgcactcc caagcggggc gggggcttcg cgacccgctc
     1441 ttcctcccgg agagcggctg gggagtttcg tacgacgcgt cgctcatcac ggctccggcg
     1501 cgcttccgcg tccagacgct cgattcaggt tccgtccggg aggatggctc tgtctgggtg
     1561 ctgccgcaga gctgggccgg acacgagaag acggtccggc aggcggtgat ggcggacgct
     1621 ctgattcggg agcttcgcag cggcctgtcg ggagaggatc cgtacggtgt ctttgcctgc
     1681 ggcaagagcg ctgtgaaggt gttcgccgcg gcgcgcccga acctcggggt tgccggagtg
     1741 aaaatcctgc ggtacggcat gtcctccctt tcgatcttcg gcgattttga cgatgtgcag
     1801 ttcgatctgt tcaccgaact cgcttccaat gcctattttt cgagcaagtt acccgcgctg
     1861 gttgcacagc ttttgacagg tacgttcggc tcggtgacga cggagatgct ttctcttctg
     1921 aagagcattt tcaacgcagc ggagtggggg gcggagttct tctcgacgtc gattttcgac
     1981 gcgccttcgg atgtctatgc ggtcgacctg ctgaacgaca tcgccgtgtc agacagtttc
     2041 ttcctgcagt ccgcggagct tctgcaagct tccggagaga agctgcgggc ggctgtatcg
     2101 gcttttgagc agaacgaccc ggaggcgtgt gcggcggcct tcggca
//
LOCUS       DAOSWW010000461         2144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41659_length_2144_cov_3.905697, whole genome shotgun sequence.
ACCESSION   DAOSWW010000461 DAOSWW010000000
VERSION     DAOSWW010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41659_length_2144_cov_3.905697"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PK442_09195"
     CDS             complement(<1..364)
                     /locus_tag="PK442_09195"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HOO87868.1"
                     /translation="MPRRFSFILAALLTLVLIPYFPGISCAEPGYSFDPSLAPSSPDY
                     SLPKSWAVLPEEPLPYPVDIFFVHPTTYNGDENWNQSLEGESADPKVRGVMKSQAGVF
                     STSACLYAPYYRQANLAVL"
     gene            complement(544..681)
                     /locus_tag="PK442_09200"
     CDS             complement(544..681)
                     /locus_tag="PK442_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87869.1"
                     /translation="MRLDADGPDALFDGRENVVAGIFETLDAINSGLYDERTSLRVAR
                     K"
     gene            859..1242
                     /locus_tag="PK442_09205"
     CDS             859..1242
                     /locus_tag="PK442_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87870.1"
                     /translation="MEKVSPENFSFDIIRFFRYPKIFWDDKEIRNASPSELKRLIDRL
                     RSSPVPLDRLRFKAFVRRMIERGSASDALLFLTPDEIREFLEQESVYCVDERMESLWK
                     KISGLPESFQVKKVRAGKPRSSGLS"
     gene            1158..1895
                     /locus_tag="PK442_09210"
     CDS             1158..1895
                     /locus_tag="PK442_09210"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOO87871.1"
                     /translation="MEKNLRPAGIIPSEKSAGWEAPEQRALLVEIFKIPLFSESFFLG
                     GGTCLSVMYFGHRQSQDLDLFTVDEISIPVAWGMLARRLPRRGYALETTISISEGFAS
                     GVFQKNGVMTKVDLVQDMFVEDLRREQVVLDGVPIRVDNFGNLVTGKFSAFLSRGEPK
                     DIIDLSRILNHAKDTGQFIPFLERLLTETSKRDALADDDTLLSEIFTKIERQHSDLFH
                     PEIARMVLAFLETLQRRKKALSEGEHS"
     gene            complement(1937..>2144)
                     /locus_tag="PK442_09215"
     CDS             complement(1937..>2144)
                     /locus_tag="PK442_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87872.1"
                     /translation="VMEPSALYEPPFTRIDSGGPDALFDGRENVVAGIFETLDAINSG
                     LYDEGTSLRVARGKSGGTMHCESV"
BASE COUNT          527 a          572 c          555 g          490 t
ORIGIN      
        1 ccagcacagc caggttcgcc tgacggtagt atggggcgta gagacaagcc gatgttgaaa
       61 aaactcccgc ctggctcttc ataacccctc gtacctttgg atcggcgctc tcgccctcaa
      121 gggactggtt ccaattttca tcgccgttat acgtcgtagg gtgaacaaaa aagatatcca
      181 ccgggtaggg aagaggctct tcgggcagaa cggcccagct tttcggcaag gaatagtccg
      241 gggaggaagg agccagagac gggtcgaaag aataccccgg ttccgcacag gaaattcctg
      301 gaaaatatgg tatcaggacg agagtaagca acgcggcaag aataaaagaa aaccttctgg
      361 gcatcagtat tcactccttt aatttgtatg tacagtttta gatgcagcga tcgagcataa
      421 atcgaatctt ctccaatgtt ttgtgtactt tcttccgtca aaaccggcgt cggcaagccg
      481 gaaccttttc gggcctcctc tcacagacca gccgttcata atgcccctcc cccttttact
      541 gaattatttc ctcgcgacgc ggaggcttgt gcgctcgtcg tacaggccgg aattgatggc
      601 gtccagcgtc tcgaagatgc ccgcgacgac gttctccctg ccgtcgaaga gggcgtcggg
      661 gccgtctgca tcaagtcgca acgatgaaaa atctcgttcg ccccactcca acatagctgt
      721 attatagaag caagaccgcc ggattcaaga gtctatcgca caacctggat gccgcccctc
      781 ggcaacgttt ttgaacagtc tccgacatga tatactcgaa tcatagagtt cccgaccatc
      841 ggaaaggagg ttgagacgat ggaaaaggtt tcgccggaaa acttttcgtt tgatatcata
      901 cgttttttcc gctacccgaa aatcttctgg gacgacaaag agatccggaa cgcatcgcca
      961 tcggagctaa aacgcctgat cgacagactg cgttcttctc cggttccatt ggaccgactc
     1021 cgcttcaagg cgttcgtccg cagaatgatc gagcgaggca gcgccagcga cgcgctgctc
     1081 tttctgacac cggacgagat cagggagttt cttgagcagg aaagcgttta ctgcgtggat
     1141 gaaaggatgg agagcctatg gaaaaaaatc tccggcctgc cggaatcatt ccaagtgaaa
     1201 aaagtgcggg ctgggaagcc ccggagcagc gggctctctt agttgagata ttcaagatac
     1261 cgcttttcag tgaaagcttc ttcctcggag gcggtacctg cctctcagta atgtatttcg
     1321 gccatcgtca aagccaggat ctcgacctgt tcacggttga tgagatttca atccctgttg
     1381 cgtgggggat gctcgccagg cgtcttccca gaagaggcta cgccctggaa acaacgattt
     1441 caatcagcga aggattcgcg tccggagtct ttcaaaaaaa tggcgtgatg acaaaagtgg
     1501 accttgttca ggacatgttt gtcgaggatc tgcgccgtga acaggtcgtt ctcgacggag
     1561 ttcctatacg agtggacaat ttcggtaatc ttgtcactgg gaaattctcc gcctttctaa
     1621 gcagaggaga accaaaggat atcatcgatc tctcgcgtat actgaatcat gcaaaggata
     1681 ccggtcagtt tatcccgttc ctggaacgcc tgctgacaga aacatcgaaa agagacgctc
     1741 ttgccgacga cgacactctt ctcagcgaaa tcttcaccaa gatagaacga cagcactccg
     1801 acctgtttca cccggaaatc gcccgtatgg ttcttgcttt tttagaaact ttacagcgca
     1861 gaaaaaaggc tctttcagag ggagagcatt cctgaagcct ctctttttca aaccacaaac
     1921 acctgccatt aacagattat acgctctcgc aatgcatcgt tcccccgctc tttcccctcg
     1981 cgacgcggag gcttgtgccc tcgtcgtaaa ggccggaatt gatggcgtcc agcgtctcga
     2041 agatgccggc gacgacgttc tccctgccgt cgaagagtgc gtcggggccg ccagagtcga
     2101 tgcgggtgaa cggaggctcg tagagggcgg acggctccat cacg
//
LOCUS       DAOSWW010000462         2143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41701_length_2143_cov_3.609195, whole genome shotgun sequence.
ACCESSION   DAOSWW010000462 DAOSWW010000000
VERSION     DAOSWW010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41701_length_2143_cov_3.609195"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..898
                     /gene="codY"
                     /locus_tag="PK442_09220"
     CDS             125..898
                     /gene="codY"
                     /locus_tag="PK442_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HOO87873.1"
                     /translation="MKDLLDKTRQVGRALQSRREGTRPDYNKLAKLLCEFSTANVYLI
                     NREGKILGHSWVSEYHSEEVSNFLEKGFMPEVFVEKMNQHRETILSETDGYLFDDDNG
                     NAPEKHMLYIPIYGAAERLGTLLLVRFFDPFSMRDLVLAEYLATLVGIEILHERTKNI
                     EERARERLIVQMAMRALSYSEVESVKHIITELGSFEGVVIASKVADRVGVTRSVIVNA
                     LRKLESAGIIESRSLGMKGTFIKVLSPLFVEELGVLQGE"
     gene            1020..1421
                     /gene="flgB"
                     /locus_tag="PK442_09225"
     CDS             1020..1421
                     /gene="flgB"
                     /locus_tag="PK442_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOO87874.1"
                     /translation="MISDFNWKVMEKTMEGLSRRFESTSRNLANANTPEYARRNVSFE
                     DQLREVVDGPRSLPMTITNEGHIPSGPMAVSAVVPEEIKIYDEKFRLDGNNVDPEREM
                     AVLSQTRMAYNAMSRFVAKKGSLYRLVIGGR"
     gene            1424..1843
                     /gene="flgC"
                     /locus_tag="PK442_09230"
     CDS             1424..1843
                     /gene="flgC"
                     /locus_tag="PK442_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOO87875.1"
                     /translation="MRMFRSIDVSGSALTAHRLWMDSISSNLANVNTTRTPEGGPYKR
                     RVPVFAEMLDKTIGGYEDIGGVRVTEIAHDNTPPRLAYQPEHPDANAEGYVAFPNVNV
                     VREMTDMLVASRAYEANLAVADAARNMWAGALEIMRG"
BASE COUNT          501 a          615 c          618 g          409 t
ORIGIN      
        1 tccgcagcgc cggatgaact atcaggaggg atttacagtg gctacagcaa agaaaagcac
       61 caagatcaca agtgaacaga acgcagcgga acttctcccc caggttatcg acaacgagcc
      121 ggggctgaag gatcttctcg acaagacgcg ccaggtcggc cgggcgctcc agagccgcag
      181 ggaggggacc cgccccgatt acaacaagct cgccaagctc ctctgcgagt tttcgaccgc
      241 caacgtctac ctcatcaaca gagaaggcaa gatcctcggg cactcctggg tctccgagta
      301 tcattcagaa gaggtctcca acttcctcga gaagggattc atgccggagg tcttcgtcga
      361 gaagatgaac cagcaccgcg agaccatact cagcgagacc gacggctacc ttttcgacga
      421 cgacaacggc aacgcgcccg agaagcacat gctctacatt cctatttacg gcgccgcgga
      481 gcgcctgggc acgctgcttc tcgtccgttt cttcgatccg ttctccatgc gcgacctcgt
      541 gcttgccgaa taccttgcca cgcttgtggg catcgaaatt ctccacgagc ggacgaagaa
      601 cattgaggag cgcgcccgcg agcgcctgat cgtgcagatg gccatgcgcg cgctttccta
      661 ctcggaagtg gagtccgtca aacacatcat caccgagctc ggctccttcg agggcgtcgt
      721 catcgcgagc aaggtcgccg accgcgtggg cgtcacccgc agcgtcatcg tcaacgccct
      781 ccgcaagctt gaaagcgccg ggatcatcga aagccgcagc ctcggcatga agggaacctt
      841 catcaaagtg ctcagccccc tcttcgtcga ggagctgggc gtgctccagg gagagtaaca
      901 ccgaagacga aagtctgtgg tatgatatga gccggcgggg aaaaccgccg gcttttgctc
      961 aagtccggag cacggagagc cgaaactgag aaagagcgtt ttcagggagg gatacggcga
     1021 tgatcagtga ttttaactgg aaggtaatgg aaaaaacgat ggaggggctg tcgagacgat
     1081 ttgaatccac ttcgcgcaac cttgccaacg ccaacacccc cgagtacgcc cgccgtaacg
     1141 tctccttcga ggatcagctc agggaggtcg tcgacggtcc gcggagtctg ccgatgacaa
     1201 ttacgaacga agggcacata ccctcgggac cgatggcggt cagcgcagtt gttcccgagg
     1261 agataaaaat atacgatgaa aaattccgtc tcgacgggaa caatgtggac cctgaacgag
     1321 agatggcggt actgtcacag acgcgaatgg cctacaacgc catgtcgcgg ttcgttgcaa
     1381 aaaaaggctc gctctatcgc cttgtcatag gaggacgatg accatgagaa tgttccgttc
     1441 catcgatgtt tccggaagcg cgctcacggc gcaccgtctg tggatggact ctatttcgtc
     1501 gaacctcgcc aacgtgaaca ccacgcgtac ccccgaggga ggaccgtaca agcgacgtgt
     1561 acccgtcttc gcggagatgc tcgacaagac catcggcggc tacgaggata tcggcggcgt
     1621 ccgcgtgacc gagatcgcgc acgacaacac gcccccccgg cttgcgtatc agccggagca
     1681 tcccgatgcc aatgcggagg ggtatgtcgc cttcccgaac gtgaacgtcg tcagagaaat
     1741 gaccgacatg ctcgttgcca gcagggcgta cgaagcgaat cttgcagtgg cggacgctgc
     1801 ccggaacatg tgggccgggg cgcttgagat tatgcgcggt tgatgttttt tttcgaattt
     1861 ccgaaaaggg gaatctctct cctctttacg aaagagtctc aggactcatg acctgtggaa
     1921 cggggggcgc acacgactgc ctcccttttt tgttataacc ttgcccgttg tgttaaaata
     1981 cgttccagta actcttaccg cgaagaggag gagattcaag tggacggctt gaagctcgat
     2041 ttaatgaaaa tggcggggat ccaatctctg ccggctgtag ctgccgtttc tgaaaagaag
     2101 gacgcgcctc ggggaaccgg actctctttc gaagacctcc tgg
//
LOCUS       DAOSWW010000463         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41772_length_2141_cov_2.839406, whole genome shotgun sequence.
ACCESSION   DAOSWW010000463 DAOSWW010000000
VERSION     DAOSWW010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41772_length_2141_cov_2.839406"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PK442_09235"
     CDS             complement(<1..634)
                     /locus_tag="PK442_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HOO87876.1"
                     /translation="MNILILGANGFIGSHLIKGVLESTDWRINAFDLSGTNLEQYRNS
                     TNFSFTQGDIFTADEWLKKAVEAADVVLPLAGIAKPSYYLSKPLWTFELDFEQNLKIV
                     RMCVAADKKVIFPSTSEVYGMSTDTVLKEEESPLIVGPINKMRWIYSCGKQMMDRVIV
                     AYGQEKGLRYTLFRPFNWIGPRLDTFKDAEERTARSITQMVYDVLSGRGIT"
     gene            complement(710..1639)
                     /locus_tag="PK442_09240"
     CDS             complement(710..1639)
                     /locus_tag="PK442_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HOO87877.1"
                     /translation="MSKLLAVVFAYSEVGHTCLKELLRQGTSVQAVFTHEDNPAETLW
                     FPSVAALAREAGIPVFTPDSLGDEEHALLLSLRPDVIFSFYYRSMIPERFLAIPPKGA
                     FNMHGSLLPKYRGRACVNWAVLNGETVTGATLHHMVRRADAGDIVDREAVPILPDDTA
                     LDVSRKVAEAAGIILERTLPSIALGAAPRTPQNEGEATYFGRRTPADGVIDWAESAVR
                     IHNLVRAVTRPFPGAFTFLRGKKLFIWKTQTGEHPSFDTPPGTVLSEDPLRIFTGRGV
                     LRLLEWQFEDSRAQSPGAGDILGKEPEGETRPS"
     gene            complement(1632..>2141)
                     /locus_tag="PK442_09245"
     CDS             complement(1632..>2141)
                     /locus_tag="PK442_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO87878.1"
                     /translation="AYRFRFDNGGKDYGCMLRAYRRDIVNIINQSCESTTFIPALAQK
                     FAVNPVEIPVGHTEREHGTSKYSIFRLIRLNFDLMTGFSLFPLQAVTMIGIVVSALSF
                     VFALYLLLRRIIVGPEAEGVFTLLNINFFLMGITMSCVGITGEYIGRIYQEVRKRPRY
                     VIRREWNDE"
BASE COUNT          393 a          617 c          710 g          421 t
ORIGIN      
        1 gggtgatccc ccgtccggag agcacgtcgt agaccatctg tgtgatggag cgggcggtgc
       61 gctcctcggc gtccttgaag gtgtcgagac ggggaccgat ccagttgaac gggcggaaga
      121 gcgtgtagcg cagccccttc tcctggccgt aggcgacgat gaccctgtcc atcatttgtt
      181 tgccgcagct gtagatccag cgcatcttgt tgatcgggcc gacgatgagc gggctttctt
      241 cctccttgag gacggtgtcg gtgctcatgc cgtagacttc cgaagtggag ggaaagatga
      301 cttttttatc cgcggcgacg cacatgcgga cgatcttgag gttctgctcg aagtcgagtt
      361 caaaggtcca caatggtttg ctgaggtagt aggagggctt tgcgatgccc gcgagcggca
      421 ggacgacgtc ggctgcttcc actgcctttt tcagccattc gtccgcggtg aagatgtctc
      481 cctgcgtgaa ggagaagttc gtgctgttcc ggtactgctc aaggttcgtc cccgaaagat
      541 cgaaagcgtt gatccgccag tccgtactct ccaggacccc cttgatgaga tgggagccga
      601 tgaagccgtt ggccccgaga atcagaatgt tcatagagaa acctccctgc gtgttccccg
      661 cgttgtgcgg gatggttcgt tcgttgaaaa aagtcgccac gagagtatat cacgaagggc
      721 gcgtttcgcc ctccggctct ttgcccagaa tatctcctgc gcccggtgat tgcgcccggg
      781 agtcctcgaa ctgccattcg aggagacgaa gaacgccccg tcctgtgaaa atgcggagcg
      841 gatcctccga gagcaccgtc cccggaggcg tatcgaaaga cggatgctcc ccggtctgcg
      901 tcttccagat gaagagcttc ttgccccgga gaaaggtgaa ggcgcctgga aaggggcgcg
      961 tcaccgcgcg gaccaggttg tgaatccgta cggcgctctc cgcccagtcg atcacgccgt
     1021 ccgcgggcgt cctgcggccg aagtacgtcg cctctccctc gttttgcggg gtccgcggcg
     1081 cggcgccgag ggcgattgag gggagcgtcc gctccagaat gatccctgcg gcttcggcca
     1141 ctttccggga gacgtcgagg gccgtatcgt cggggaggat gggcacagcc tcccggtcga
     1201 cgatatcccc cgcgtcggct cgtcgtacca tgtggtggag cgtcgcgccg gttaccgtct
     1261 cgccgttgag gacggcccag ttgacgcagg cccgtcccct gtacttcggc agcagcgagc
     1321 cgtgcatgtt gaaggccccc ttcggcggga tcgcgaggaa gcgctcgggg atcatcgagc
     1381 ggtagtagaa agagaagatg acgtcggggc gcagagagag cagcagcgcg tgctcctcgt
     1441 cgccgaggga gtccggggtg aagacgggga ttcccgcttc ccgggcgagc gccgccaccg
     1501 aggggaacca gagcgtttca gccgggttgt cctcgtgggt gaagactgcc tgtacggatg
     1561 ttccctgacg cagcagctcc ttcaggcacg tgtgaccgac ctcgctgtag gcgaagacga
     1621 cggcaagaag cttactcatc gttccactcc ctgcggatga cgtagcgggg gcgtttgcgg
     1681 acctcctggt agatgcgtcc gatatactcc cccgtgatgc cgacgcagga catggtgatg
     1741 cccatcagga aaaagttgat gttgagcagc gtgaagacgc cctccgcttc cgggccgacg
     1801 atgattctgc ggagcagcag gtagagtgcg aagacgaagc tcagcgcgga gacgacgatg
     1861 ccgatcatcg tcaccgcctg aagcgggaag agagaaaacc ccgtcatcag gtcgaagttg
     1921 agccgaatga ggcggaagat gctgtacttc gacgtgccgt gttcccgctc cgtatgcccc
     1981 acagggattt cgaccgggtt gacggcgaac ttctgcgcca gcgcggggat gaaggtcgtg
     2041 ctctcgcagc tctggttgat gatattcacg atatcgcgcc ggtaggcccg gagcatgcag
     2101 ccgtagtcct tgccgccgtt gtcgaagcgg aagcgatagg c
//
LOCUS       DAOSWW010000464         2136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41907_length_2136_cov_4.378184, whole genome shotgun sequence.
ACCESSION   DAOSWW010000464 DAOSWW010000000
VERSION     DAOSWW010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41907_length_2136_cov_4.378184"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2136)
                     /locus_tag="PK442_09250"
     CDS             complement(<1..>2136)
                     /locus_tag="PK442_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOO87879.1"
                     /translation="PRFLELAGQSIRELLREGVPREELAKALGCGGCAPGTRCERLGD
                     ERAASRNERASDAGSALLCALYGDYIALLEERGLADSAQIPSLGAALLEAFPQEGAGW
                     ASGLKLRAAGFLSFASGQMAFLHSLCAAGADLELWVPECGEGDFYTAVQQFPEAELLR
                     CSGESDAVPPLCVSMAAGDLRLSTDTLARELFLWPAGEGYLSAASGLSFPGWENIALC
                     AESEELNSLVESFTRYGIPYSLREGATVSETALWKSALRALDLAADGWPPVETADFLS
                     SLLFAPFSFPRRAFGNALPSGKKGWTGFLEEFPPEAGRDAFRRATAFADVVQRGGRPE
                     ELLSAFAHLAPSRAELKLLVERAARFPRLDGHIREVSLAVQEANEKERSLRELRRDLG
                     DAGSARLSFEEAVAFLGQWAETASIWMPSPVSPTVAIHPGTPPSLVRAPVWILPGMTA
                     KRWPGQVHESPLLSDERKEFLHESLELGRSHLPLVPEKRSQREALFRRLAACGESLCV
                     LLRPLADDGGRPLPESPFVDDACSSSHPWLRSSGAEPLKRSLGEILAPEEYPVAEGVE
                     TRWRSWATTGAARGSLKEIPLLPPKEKVFSLSDLDTYVSCPFRYYCARTASLDTPPEE
                     LHRFDLAGSALHLLWQKVWEKRLSTGEQLDVLVNGLFDEAMDEKYARLLANANLKRDR
                     KELLRRAVRLARAQQTMEDGGLASERLEQ"
BASE COUNT          366 a          754 c          712 g          304 t
ORIGIN      
        1 tctgctccag ccgctccgag gccagcccgc cgtcctccat cgtctgctgc gcgcgcgcca
       61 ggcgcactgc gcggcgcaac agctccttcc ggtcgcgctt gaggttcgcg ttcgccagaa
      121 ggcgggcgta cttctcgtcc atagcctcgt cgaagaggcc attgaccagg acgtccagct
      181 gctctccggt cgaaaggcgt ttctcccaca ccttctgcca cagcagatgg agcgcgcttc
      241 cggcgaggtc gaaccggtgc agctcctcgg ggggcgtgtc cagcgacgcc gtgcgggcgc
      301 agtagtaacg gaaggggcat gaaacgtacg tgtccaggtc gctcagtgaa aacaccttct
      361 ccttcggagg gaggagggga atctctttca ggctgccccg ggccgcgccg gtcgtcgccc
      421 acgagcgcca gcgcgtctcc acgccctccg ccacagggta ctcctccggg gcgagaatct
      481 cgccgagcga gcgtttcagc ggctccgctc ccgaagagcg cagccacgga tgcgacgaag
      541 agcaggcgtc gtcgacgaag ggggattcgg ggagcggacg gccgccgtcg tcggccagag
      601 ggcgcagcag aacgcagagg ctctcgccgc aggccgcaag gcggcggaag agcgcctcgc
      661 gctggctccg cttctccggc accagcggca ggtggcttcg cccgagctca aggctttcgt
      721 gcaggaactc tttccgttcg tcgctcagaa gaggggattc gtgcacctga cccggccacc
      781 gcttcgccgt catcccggga agaatccaca caggggcgcg gacgagcgag gggggcgtgc
      841 cggggtgaat ggcgacggtc ggagagaccg gcgaaggcat ccagatcgac gccgtctccg
      901 cccactgccc gaggaaggcc acagcctcct cgaaagagag acgcgcgctt cccgcgtcgc
      961 caaggtctct gcgcagttcg cgcagggaac gctccttttc gttcgcctcc tgcacggcga
     1021 gcgacacctc gcggatatgc ccgtcgaggc gggggaagcg cgccgcccgt tcgacgagca
     1081 gcttcagctc cgcgcgggag ggcgcaaggt gcgcgaaggc cgacaggagc tcctcagggc
     1141 gcccgccgcg ctgcacgacg tcggcgaagg ccgtcgcccg gcggaacgcg tccctcccgg
     1201 cctcgggggg gaattcctcc aggaagcccg tccacccttt tttgcccgaa ggcagcgcat
     1261 tcccgaatgc ccgtcgcggg aagctgaacg gagcgaagag cagcgacgaa agaaagtccg
     1321 ccgtctctac ggggggccac ccgtccgcgg ccaagtcgag cgcccgcagc gcgctcttcc
     1381 agagcgccgt ttcggagacc gtcgctccct cgcgcagcga gtagggaata ccgtaccggg
     1441 tgaagctctc gacgagggag ttcagctcct cgctctccgc gcagagggcg atgttctccc
     1501 accccggaaa ggagaggccg ctcgccgcgg agagataccc ctcgcccgca ggccagagaa
     1561 agagctcgcg agcgagcgtg tccgtcgaga ggcggaggtc gcccgccgcc atggagacgc
     1621 agagaggcgg cacagcgtcg ctttctccgg aacagcggag aagttcagcc tcggggaact
     1681 gctgcaccgc ggtgtaaaag tctccttcgc cgcactcggg aacccagagc tccaagtccg
     1741 ctcccgcagc gcacagcgag tgcagaaacg ccatctgccc cgaagcgaag ctcaggaatc
     1801 ctgcggcgcg cagcttcaaa ccgcttgccc atccggcccc ctcctgcgga aacgcctcca
     1861 gcagcgccgc tcccagcgag gggatctgcg cgctgtcggc gaggccgcgt tcttcgagga
     1921 gagcgatata gtcgccgtag agcgcgcaaa gaagcgcgct ccccgcgtcg ctcgcacgtt
     1981 cgttccggga ggctgcacgt tcgtcgccca gacgttcgca ccgcgtaccc ggagcacagc
     2041 cgccgcaccc cagcgccttc gccagctcct ccctcggcac gccttccctg agcagttcgc
     2101 ggatggactg tcccgcgagc tccaagaagc gcggag
//
LOCUS       DAOSWW010000465         2133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_41996_length_2133_cov_4.042830, whole genome shotgun sequence.
ACCESSION   DAOSWW010000465 DAOSWW010000000
VERSION     DAOSWW010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41996_length_2133_cov_4.042830"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PK442_09255"
     CDS             <1..402
                     /locus_tag="PK442_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO87880.1"
                     /translation="GMFIQVMTLIGVQGFLVVSVLALPAWLMYLGMATSIPLFGAVSA
                     FGAASVLGVPFLLALLGRNEIMVGSALSLLAGLGDLMPPTALAGIFAAQVVGEENYFK
                     VLRYCLIPALVTALWAVFVILNANTIMGFLP"
     gene            416..559
                     /locus_tag="PK442_09260"
     CDS             416..559
                     /locus_tag="PK442_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87881.1"
                     /translation="MTLIYRAIVLFVLLLTVRCQFREKDFWKQATAALVVVPLVLRLL
                     MIK"
     gene            575..1708
                     /locus_tag="PK442_09265"
     CDS             575..1708
                     /locus_tag="PK442_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOO87882.1"
                     /translation="MKLKGTPLTAVLLLLAAFAVAALAGLNFKAMWAEDDFVPAQGFE
                     KKMLSAWFEGLAGTPADTPVYIQKGTEPGGVVLILGGTHPTEPASMVTATLFLERAQV
                     TKGSLIVIPQANLLGFTHNSPQEAHPQSISFPTADGGKRTFRFGSRLTNTIYEWPSPD
                     IYIHPASGQQLPGKERGNLNRCYPGVPDGSITEQVAYALTELVRRERVDLAFDLHEAS
                     PEYPVVNAIVAHERSMELAAMTTMDLEAAGIMMRLEPSPKNLRGLTHREWGNFTETMP
                     VLMETGNPVQGRLRGRTDERLALTGADKAYVKAAALGRLYIPYEGDQKIEYRVGRHAT
                     AIMSFLLNLDLLNEERGVLLENIPTFEELQENGVGAYLAPLKL"
     gene            1899..>2133
                     /locus_tag="PK442_09270"
     CDS             1899..>2133
                     /locus_tag="PK442_09270"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOO87883.1"
                     /translation="MIFVLAGSAGAADFEVKEVLAGEGMVSSAHELASQAGVEILQKG
                     GNAIDAAVATMLALNVVESNASGIGGGGFMTIRF"
BASE COUNT          412 a          578 c          645 g          498 t
ORIGIN      
        1 ggcatgttca tccaggtcat gacgctgatc ggcgtccaag gattcctcgt cgtctccgtg
       61 ctcgcccttc cggcgtggct tatgtacctt gggatggcga cgtcgatccc tcttttcggg
      121 gccgtctcgg cgttcggcgc cgcgtcggtg ctcggcgttc ccttcctgct ggcgcttctg
      181 ggaaggaacg aaataatggt tggatcggcg ctcagtctcc tcgcaggcct gggcgatctc
      241 atgcctccca ctgcgcttgc aggtattttc gcagcccagg ttgtcgggga ggagaactat
      301 ttcaaggtgc ttcgctactg cctgatcccg gcattggtaa cggcgttgtg ggcggtgttc
      361 gtcatcctca acgcgaacac gatcatgggc tttctgccgt aaggaggaga aaactatgac
      421 tctgatctat cgtgcaatcg ttctttttgt cctcctcctg acagtgcgct gccagttcag
      481 agagaaggat ttctggaagc aggcgacggc tgctctggtt gttgtccctc tggtattgcg
      541 tctcttgatg atcaagtagg ggagggtgaa gacaatgaaa ttgaaaggta ctccgttgac
      601 agccgttctt ctccttcttg ccgcgttcgc ggtcgcagcg cttgccggac tgaacttcaa
      661 ggcgatgtgg gcggaagatg attttgttcc ggcgcagggc ttcgagaaga aaatgctctc
      721 cgcctggttt gaagggctgg cgggaactcc cgcggatact cctgtctaca ttcagaaggg
      781 aacggagcct ggcggcgttg tgcttatcct gggcggaacc catccgacgg agcctgcctc
      841 catggtcacc gccacgctct tccttgagcg ggcgcaggtg acaaaaggaa gccttatcgt
      901 cattcctcag gcgaatctgc tggggtttac gcacaactct ccccaggaag cgcatccgca
      961 aagcatctct tttcccactg cggacggcgg caagaggacg ttccgtttcg gctcaaggct
     1021 caccaatacg atctacgagt ggccctcgcc ggacatttat attcacccgg cttcggggca
     1081 gcagcttccg gggaaagaac ggggtaatct caaccggtgc tacccgggag ttccggacgg
     1141 ctctatcacc gaacaggtcg cgtacgcgct cacggagctt gttcgcaggg agcgcgtcga
     1201 tctcgcgttc gatctccatg aggcctcccc ggagtacccc gtcgtgaacg ccatcgtcgc
     1261 gcatgagcgc agtatggagc ttgccgccat gacgacgatg gatctggaag ccgcgggtat
     1321 tatgatgcgc cttgaaccct cgccgaagaa cctccggggg ctgacgcaca gggagtgggg
     1381 aaatttcacg gagacgatgc ccgtgcttat ggaaacagga aatcccgtcc aaggccgcct
     1441 tcgcggcaga acggacgaga ggctggcgct cacgggcgcg gacaaggcgt acgtgaaggc
     1501 cgccgctctt ggccgcctct atatcccgta cgagggcgat caaaagatcg aataccgggt
     1561 tggacggcat gcgacggcta ttatgtcttt cctcctcaat ctcgatctgc tcaacgaaga
     1621 gagaggggtg ctcctggaga acatcccgac atttgaagag ctccaggaga acggggtcgg
     1681 cgcgtatctt gctcctctga aattgtagtt gttgcggcgc gaatcctgct tttcgttttt
     1741 tattatataa taggtaagaa tcaggacgat acacgagttc agaggtgtgt ttcgcttcaa
     1801 aagttccact gaaagaaaca tgcctccgga gcacggcaat aatatatctt ttcaggaggt
     1861 gttgtattgt gagaaagagt tttgtcgcgt tgttccttat gatttttgtt ttagcgggaa
     1921 gcgccggtgc ggccgacttc gaagtgaagg aggttctcgc cggggaggga atggtctcct
     1981 ccgcgcatga actcgcttcg caggccggag tggagattct gcagaagggc ggcaacgcca
     2041 tcgacgccgc cgtggcgacg atgcttgcct tgaacgtggt cgaatccaat gcctccggta
     2101 tcggcggcgg cggatttatg accatccgct tcg
//
LOCUS       DAOSWW010000466         2133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42003_length_2133_cov_3.098171, whole genome shotgun sequence.
ACCESSION   DAOSWW010000466 DAOSWW010000000
VERSION     DAOSWW010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42003_length_2133_cov_3.098171"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1715
                     /locus_tag="PK442_09275"
     CDS             126..1715
                     /locus_tag="PK442_09275"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOO87884.1"
                     /translation="MLCAMLCVILLSSAALACTVLAVGKDATVDGSAMITHNDDSRTA
                     NSRLFIIPEADWPEGSMRSVVKDAHGYEGDGQVIGEIPQVPHTFRYFFSRYSFMNEKG
                     VAISESTDSVEVTDERSKKVEQIMAKDAVGMMDAWILQDIMLERASTAREAVEIMGKL
                     VEEYGWYDAPETMPLTDGNEVWIAEFYGNKIWAAWRMPDDHIFVGANRARLRHLDLTD
                     KENVMYCPDLVEFAVKNGFIEEKDVNMKDFSPADVYCPSTALYATRREWRVLSLAAPD
                     SFKLGPDEYDYPMSIKPDKKLSVHDVLRIKGDWYEGTPFDLSKGIQAGPWGNPIRFAN
                     SSKKNPSASWERSVNMMRTAYVHIAQVRGDLPVEVRGISWYGYGAPDTTYITPLWPVM
                     SKLPPLYGIGDRYHDYDPKSGWWVNTRVQELAGLRYQDARKEIHAARDAKLNPLYVQT
                     RMIQDKAAEMLKAGDKDGAVSLITDFAYAHAVDWNQRWLALGDRLLSKYALGYTDVKT
                     TPYPEEWNEIVGFDTPKRSTK"
     gene            1815..>2133
                     /locus_tag="PK442_09280"
     CDS             1815..>2133
                     /locus_tag="PK442_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOO87885.1"
                     /translation="MKLFRTLLFFILLATAADASAIEQGSASRLMARCEGVLYAEKCR
                     LGLYDAETDPEKTGLIGVEFSPLTTSQGHLPDKQAAARPAMADAVAEYLRRAGVEEGD
                     WVAV"
BASE COUNT          439 a          716 c          637 g          341 t
ORIGIN      
        1 tctcacaagg cttctcccgg cgggagcgtt tccgtcggcg caccgcgcgc ccgaaggctt
       61 tggtatgcat catgtatctc tatttcagac aggaggaaaa acacgtgttg aaatcattca
      121 gaaccatgct gtgcgccatg ctgtgcgtca tccttctctc ctcggccgcc cttgcctgca
      181 ccgttcttgc ggtgggtaaa gacgccaccg tggacggaag cgccatgatc acgcacaacg
      241 acgactccag gaccgcgaac agccgtctct tcatcattcc cgaggccgac tggccggaag
      301 gatcgatgcg gagcgtcgtg aaggacgccc acggctacga gggcgacgga caggtcatcg
      361 gcgagatccc gcaggttccg cataccttcc ggtacttctt ctcccgctac tccttcatga
      421 acgagaaggg cgtcgccatc agcgaatcga ccgactccgt cgaggtcacc gacgagcgga
      481 gcaagaaggt cgaacagatc atggcgaagg acgccgtggg aatgatggac gcctggatcc
      541 ttcaggacat catgctcgag cgcgcgagca cggcccgtga agcggtcgag atcatgggca
      601 aactcgtcga ggagtacggc tggtacgacg ctcccgagac gatgcccctg accgacggca
      661 acgaagtctg gatcgccgag ttctacggca acaagatctg ggccgcatgg cgcatgcccg
      721 acgaccacat cttcgtgggc gccaaccgcg cgcgtctccg ccacctcgac ctgaccgaca
      781 aggaaaacgt catgtactgc cccgacctcg tcgagttcgc cgtgaagaac ggattcatcg
      841 aggaaaagga cgtcaatatg aaggacttca gcccggccga cgtctactgc ccgagcaccg
      901 cgctctacgc gacacgccgc gagtggagag tcctctcgct cgccgctccc gactccttca
      961 agctcggccc cgacgaatac gactacccca tgtccatcaa accggacaag aagctcagcg
     1021 tgcatgacgt gctgcgcatc aagggcgact ggtacgaggg cactcccttc gatctgagca
     1081 agggcatcca ggccggtccc tggggcaacc ccatccgctt cgccaattcc agcaagaaga
     1141 acccctccgc cagctgggag cgctccgtca acatgatgcg gacggcctac gtccacatcg
     1201 cccaggtccg cggcgatctc cccgtggaag tgcgcggcat cagctggtac ggctacggcg
     1261 cgccggacac cacctacatc acccccctgt ggcccgtgat gagcaagctg cccccgctct
     1321 acggcatcgg cgacagatac cacgactacg atcccaaatc cggctggtgg gtgaacaccc
     1381 gcgtgcagga actcgcagga cttcgctacc aggacgcccg caaggagatc cacgccgcac
     1441 gcgacgcgaa gctcaatccg ctctacgtgc agacgcggat gatccaggac aaggcagccg
     1501 agatgctgaa ggccggcgat aaggacggcg cggtctccct gatcaccgac ttcgcctacg
     1561 cccatgccgt cgactggaac cagcgctggc tcgccctcgg cgacaggctg ctgagcaagt
     1621 acgccctcgg ctacaccgac gtgaagacca cgccgtaccc cgaggagtgg aacgagatcg
     1681 tcggcttcga caccccgaag cgctccacga aatagtattt tcctgctgat gaaagggcgc
     1741 ggagggcttg agagccgccg cgcccttttc gtatcctcgc ggcgccccgc cgcgaggccc
     1801 aaggaggcga ccgtatgaag ctcttccgaa cgctgctgtt tttcattctg ctggcaaccg
     1861 ccgccgacgc ttccgccatc gagcaggggt cggcgtcccg cctgatggcg cgctgcgaag
     1921 gcgtcctcta cgcagaaaag tgcagactcg gcctgtacga tgcggaaacg gacccggaaa
     1981 aaacaggcct gatcggcgtc gagttctcgc cccttacgac cagtcagggg catctgccgg
     2041 acaagcaggc ggcggcccgc cccgccatgg cggacgccgt cgcggaatac ctccggcggg
     2101 cgggtgtgga ggaaggggac tgggtcgccg tga
//
LOCUS       DAOSWW010000467         2131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42070_length_2131_cov_3.397881, whole genome shotgun sequence.
ACCESSION   DAOSWW010000467 DAOSWW010000000
VERSION     DAOSWW010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42070_length_2131_cov_3.397881"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PK442_09285"
     CDS             complement(<1..1049)
                     /locus_tag="PK442_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO87886.1"
                     /translation="MAKAESVRYFCSACGASSASWAGRCPSCGEWGSLEKGTAASASG
                     VSTPQKTKSVKATDVLPPERVSSGIEEFDRVIGGGWIPGGVFLLGGQPGIGKSTLLLQ
                     TCGSMAESIGSVLYVSGEESASQIALRARRLGVLSDELHLVCTNDMAAALESLPGKKF
                     LVVDSVQAMRADGEGGWPGSPAQVRAVAQMCISAAKEQSVPAVLVGHITKEGRIAGPM
                     LLEHMVDGVLTFSGEDYSPYRMLRAGKNRFGSTEELGVFEMTETGLVPVTDVSGLYWN
                     KAAETVPGVAMTVAVEGSLPLIAEIQSLASATAFPYPKRTGRGVDVNKIQLFSAVLDK
                     RCGIGCGLFDIYVNVA"
     gene            1220..>2131
                     /locus_tag="PK442_09290"
     CDS             1220..>2131
                     /locus_tag="PK442_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOO87887.1"
                     /translation="MNRLPRGCANVGGDLAAAMEECKRVFLSLFEQWGYRPFLPSGLQ
                     LLESSWERLPSLFRQKLIAVNSPYGEPCCLRGDITLATVAYLASHHSPEERPLRICYS
                     DRVYARAEKPRLAMESFQIGAELLGWERQGADAEMLFLLLRMLDEIGLEKSILLLGDA
                     TFLAKAVSEIEFSAAERIVGALQKGSLIEYAALLDEENIPDFYRAILQRIPELHGGPE
                     VLEEAAALWGRGAPLLSLKNVVSTLTALGYGDRIAIDLSLVRELGYYSGPIFDVYSEN
                     YGQSLGGGGRYDRLLSLCGLQGQAMGFG"
BASE COUNT          414 a          704 c          611 g          402 t
ORIGIN      
        1 gccacattta catagatatc gaagagcccg cacccgatac cgcagcgctt gtcgagcacc
       61 gcggaaaaaa gctgtatctt gttcacgtcc actccccgcc cggtgcgctt ggggtacggg
      121 aaggcagtcg cgctcgccag cgactgaatc tcggcaatga gaggaaggct tccctccacc
      181 gcgaccgtca tcgccactcc gggaaccgtc tccgccgctt tgttccagta cagcccgctc
      241 acatccgtga caggcacgag gccggtctcc gtcatctcga agacgccaag ctcctcggta
      301 ctgccgaacc ggtttttccc cgcccggagc atccggtagg gagagtagtc ctctccggag
      361 aaggtgagca cgccgtcgac catgtgctcg aggagcatcg gacctgcgat ccgcccttcc
      421 ttcgtaatat gcccgaccag aactgcgggc acggactgct ccttcgccgc ggagatgcac
      481 atctgcgcca cggcgcggac ctgcgccgga ctccccggcc acccgccctc gccgtctgct
      541 ctcattgcct gtacgctgtc caccacaaga aacttcttcc ccgggagcga ttcgagcgcc
      601 gctgccatat cattggtgca gacgagatgc agctcgtcgg agagcacccc cagccgcctg
      661 gcccggagcg cgatctgcga ggccgattcc tcgccggaga cgtagaggac ggaaccgata
      721 ctctccgcca ttgagccgca cgtctggagc agcagcgtcg attttccgat gccgggctgt
      781 ccgccgagca agaagacgcc ccccggaatc catccgccgc cgatgacgcg atcgaactcc
      841 tcgatcccgg aagaaacacg ctccggcgga agcacatccg tagccttgac gctcttggtc
      901 ttctgcggtg tactcacgcc ggatgctgaa gcggcagttc ctttttcaag cgacccccac
      961 tcgccgcagg agggacatct tcccgcccac gaagcggagg acgccccgca ggcagagcag
     1021 aaataccgta cggattcagc cttcgccata cctctcgcct cccagctcag tgtatctctc
     1081 ccgctttctc ctgtcgagag gctttacact tttacaatac aatgatatca tgccgtgagt
     1141 attaaagaca ttaaggtgat aatctaccca gctaaagtga ggaaatgaca atgacaatac
     1201 aaatcaagag ggtacacata tgaaccgcct tccgcgcggc tgcgccaacg tcggggggga
     1261 tcttgcggcg gcaatggaag agtgcaagcg ggtctttctt tcgctcttcg agcaatgggg
     1321 ctaccgcccg ttcctgcctt cgggtcttca gcttctcgaa tcttcatggg agcggctgcc
     1381 gtcgctcttc cgacaaaaac tcatcgcggt gaactccccg tacggcgagc cgtgctgcct
     1441 tcgcggagac atcacgctgg cgaccgtcgc gtacctcgcg tcgcaccact cgcccgagga
     1501 gcgtccgctg cgaatctgct actcggacag ggtgtacgcg cgggcggaga aaccccgcct
     1561 tgcgatggag tcgttccaga tcggcgcgga gctgctcggg tgggaacggc agggagcgga
     1621 cgcggagatg ctcttccttc ttctccggat gctcgacgaa atcggactgg agaagagcat
     1681 cctcctgctg ggcgacgcaa cgtttctggc aaaggctgtc tccgagatcg aattttcggc
     1741 cgccgagagg atcgtcggcg cccttcagaa aggatcgctg atagaatacg ccgccctcct
     1801 cgacgaggag aacatccccg acttctacag ggctattctc caacgtattc ccgagcttca
     1861 cggcggtccg gaggtgctcg aagaagcggc ggcgctctgg ggaagaggcg ctcctctcct
     1921 ctcgctcaag aacgtcgtct ccacgctgac ggcgctcggc tacggcgacc gtatcgccat
     1981 cgacctgtcg ctcgtccgcg aactcggcta ctacagcgga ccgatcttcg acgtctattc
     2041 cgaaaactac ggacagtctc tcggaggcgg agggaggtac gaccggctgc tctcgctctg
     2101 cggactccag gggcaggcga tggggttcgg a
//
LOCUS       DAOSWW010000468         2131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42082_length_2131_cov_2.759634, whole genome shotgun sequence.
ACCESSION   DAOSWW010000468 DAOSWW010000000
VERSION     DAOSWW010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42082_length_2131_cov_2.759634"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK442_09295"
     CDS             complement(<1..594)
                     /locus_tag="PK442_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO87888.1"
                     /translation="MDTNRSARRIQKDLEAINRPARNPGGGITRLSYSPEHAEALRYL
                     QRECSALGARCFLDEAGNFAARMEGANPELPVFCLGSHVDSVKNGGELDGMAGVIIGL
                     EMFRLLKESGMQLRHGIQLMGFAEEEGVLCNMGLLGSRFFCGEITPEDFRKLRTGSGL
                     LLGDLAENFCASLLPLMDGPFPKEAKNLAFFLEPHIEQ"
     gene            1064..1870
                     /locus_tag="PK442_09300"
     CDS             1064..1870
                     /locus_tag="PK442_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOO87889.1"
                     /translation="MMLKYYVADAFAEEVFEGNPAGVCILDEWLPEDLMTRIAMENNL
                     SETAFAVKEDDCTYGLRWFTPVGEIDLCGHATFGTAFVLFRFEEQRADTLHFRAKKCG
                     YHLIVRKNGDMLTMQFPTIVPEPYVYAEYMGEGLGAVPSEVYRTERDLLLVYDSDETI
                     RNMKPDFSKLKEFPVGLSVYVTAKSGSSEFDIVARAFWPKINVNEDPVCGTMYCALMP
                     FWRERLGKDTLVARQLSARGGTVYCSYNGDSVSISGRGALYSIGNILVDA"
     gene            1882..>2131
                     /locus_tag="PK442_09305"
     CDS             1882..>2131
                     /locus_tag="PK442_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87890.1"
                     /translation="MILEISKTEAAGLYEISVEDVCAQGISVNIPGTEIQILQVRCRK
                     GMLFCGIFDKSVVEELQFPAAIFSAPKFEDMMKNKPIYL"
BASE COUNT          526 a          501 c          591 g          513 t
ORIGIN      
        1 ctgttcgata tggggctcca gaaaaaaggc gagatttttg gcttctttcg gaaaagggcc
       61 gtccatcagg ggaagaaggg aggcgcagaa attctctgcc agatctccca gaagaaggcc
      121 cgagcccgtt ctgagttttc gaaaatcctc cggagttatc tcgccgcaga agaagcggct
      181 tcccagaagg cccatattgc agagtacccc ttcctcttcc gcaaaaccca taagctgtat
      241 gccgtgccgc agctgcatgc cgctttcttt cagaagacgg aacatttcca ggccgatgat
      301 cacgccggcc atgccgtcca gctctcctcc gttctttacg ctgtccacgt gagaacccag
      361 gcagaagacc ggaagctccg gattcgctcc ctccatgcgg gccgcgaaat ttcccgcctc
      421 atccagaaag cagcgcgctc ccagggcgga acactctctt tggagatacc gaagagcctc
      481 ggcgtgttcc ggagagtagg aaagccgggt aatgcctcct ccgggattcc gggcggggcg
      541 gttgatcgct tccagatcct tttgaatgcg gcgagcggaa cgattcgtat ccatgggaca
      601 acctcctgaa aaaaagataa cgctaacgag aagaacgtgg aaacaattag gtaggcaatg
      661 ggtaggatga tcgagaaatg ccgcagcgaa ggaagcatcc tgaaagattt cggtttagcg
      721 gttgatttcg agatttctgg tagccagggt actccttatg caaattccct tggctattat
      781 ttttgatagt atgtataaaa aatgtgcgtc cttgtatctt tttccccaat attctatggt
      841 gatagcatgg gagagtcaac gaggacagga gaagcggtgc gtagtaccgt agataacggt
      901 ctatctttgt gatatggctg atttcccgag ggtcaacgca atatacggtg aatgcttccc
      961 gaagccctat ccggcgagaa cctgtgtgca ggtcgctaaa cttcctctga atgcgcagat
     1021 tgagattgat gtgattgtat gcaaataaag ggaaggagga aagatgatgc tgaagtatta
     1081 tgttgcggat gccttcgccg aggaggtgtt tgaagggaac cccgcgggcg tttgtatcct
     1141 tgacgagtgg cttcccgaag atttgatgac gagaatcgcc atggaaaaca atctgtccga
     1201 aaccgcattc gcggtcaaag aggacgattg cacatacggt ttacgctggt ttacgccggt
     1261 tggagagatt gacctttgcg gacatgccac cttcgggacc gcttttgttc tgtttcgctt
     1321 cgaagaacag agagccgaca cgttgcattt tcgcgcgaag aaatgcggtt atcacctgat
     1381 cgtcagaaag aacggtgata tgcttaccat gcagtttcca acgatcgttc ctgagcccta
     1441 tgtatatgcg gaatatatgg gcgagggcct cggtgccgta ccctcagagg tttacagaac
     1501 agagcgggac ttgctgttgg tgtacgattc tgacgaaacg attcgcaata tgaaacctga
     1561 cttctcgaaa ctcaaggaat ttccggtggg cctttccgtt tatgtgaccg caaagagcgg
     1621 cagcagcgag ttcgatatcg tggcgcgagc gttttggccc aaaatcaacg taaacgagga
     1681 tccggtttgc ggaaccatgt actgtgcgct gatgccattc tggcgtgagc gacttggaaa
     1741 ggatacgctg gtggcacgac agctttccgc gcgaggtgga actgtctatt gttcgtacaa
     1801 cggcgacagc gtcagcatca gcggcagagg cgcgttatat tcaatcggga acattttggt
     1861 ggatgcatga ggtgatgcga tatgatattg gaaatcagta aaacggaagc ggcggggttg
     1921 tatgaaattt ccgtggaaga tgtttgcgcc cagggcattt ccgtaaacat tcccggcacc
     1981 gaaatccaaa ttttacaggt tcgatgccga aaggggatgc tgttctgtgg cattttcgac
     2041 aagtcagttg tcgaagagct tcagttcccc gccgcaattt tttccgcccc gaagtttgaa
     2101 gacatgatga aaaacaagcc catctatctc a
//
LOCUS       DAOSWW010000469         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42167_length_2128_cov_3.382055, whole genome shotgun sequence.
ACCESSION   DAOSWW010000469 DAOSWW010000000
VERSION     DAOSWW010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42167_length_2128_cov_3.382055"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PK442_09310"
     CDS             <1..906
                     /locus_tag="PK442_09310"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOO87891.1"
                     /translation="ARKGCFTLPRGEEGKNLENLSAIYAALAAAGVDRSGTLLALGGG
                     VVGDTAGFAAATWMRGIRLLNCPTTLLAQVDSAIGGKTGVNLPEGKNLVGAFHPAEWV
                     FSDVECVRSQADEDFRQGLGEAVKYGVGEEWGFFGWLEENARAILRRSLPALRTLVAE
                     CSRMKLSVVAEDEHERSGARARLNLGHTVGHALETASGYRWRHGDGVAAGMMVAGRLA
                     HRLGEFRKEEPARLERLLEAFRIPCRPDMPWDEIAPFLARDKKFTGGAPRLVLPRTGE
                     RCRLRDDVTLAALRDAYEEELLKAR"
     gene            complement(958..2004)
                     /locus_tag="PK442_09315"
     CDS             complement(958..2004)
                     /locus_tag="PK442_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO87892.1"
                     /translation="MEKFQVMIGLVGVIALVAVGFVLNFAQAVFIPLIIAWLLSYLFG
                     PIVRYLAKRRVPVGLAVVFVLAIFFGLCFLGAIFLNTRIASFAAAFPKYYSRLMEIGK
                     SLTTNLDIPPDFWTSINWGFTIRGYLLDLSSSFVTIISKLVMVIVFLVFMLLGSPYFE
                     YKVKKAFVTPHGSERARKILSTISWQIGRYLSALALISAVTGLIVWMSLEYLQVDFAV
                     TWGVLAFILNFIPTIGSIVASIPPILVAVVQFYPSLTAPLITALTLLTIQVVIGNFIT
                     PKVMGDRLNLSPVVVLISLLFWGLIWGVVGALISVIIAAIIKIVCENFPMLNVISVMM
                     GSGKVYQKEFEEKR"
BASE COUNT          491 a          598 c          682 g          357 t
ORIGIN      
        1 gcaaggaaag gatgcttcac gcttccccga ggcgaggagg ggaaaaacct cgaaaacctc
       61 tccgcaattt acgccgccct cgccgccgca ggcgtcgacc gctccggaac gctgctcgcc
      121 ctcggcggag gcgtggtggg cgataccgca gggttcgccg ccgccacatg gatgcgcggc
      181 atccggcttc tcaactgccc gacgacgctc ctcgcgcagg tggacagcgc gatcggcggg
      241 aaaacggggg tgaaccttcc ggaggggaaa aacctcgtcg gcgcatttca ccccgccgag
      301 tgggtctttt cggacgtgga gtgcgtccgc tcccaggccg acgaagattt ccggcagggc
      361 ctgggggagg ctgtgaagta cggcgtcggg gaggagtggg gattcttcgg atggcttgaa
      421 gagaatgcgc gcgccatcct gcgccgctct ctgcccgcac tgaggacgct cgtcgcggag
      481 tgttcgcgga tgaagctctc cgtggtcgcg gaggacgagc atgaacggag cggggcgcgg
      541 gcccgtctga acctcgggca caccgtcgga cacgcgcttg aaaccgcgtc cggctacaga
      601 tggcggcacg gcgacggcgt cgcggcgggg atgatggttg ccggaaggct ggcgcaccgc
      661 ctcggcgaat tccggaagga ggagccggct cgtctggaac ggcttctgga ggcgttcagg
      721 attccatgcc gtccggatat gccgtgggac gagatcgccc cgtttctggc acgggataag
      781 aaatttaccg gtggcgctcc ccgcctggta ctcccccgca cgggcgaacg ctgccgcctg
      841 cgggacgacg tgacgctcgc cgcgcttcgg gacgcatacg aagaggagct cctcaaggcg
      901 cgctgaaacg cccggggagc tcctctcttc atattccgcc gcaggtgtcc ggacggttta
      961 gcgcttctcc tcgaactcct tctggtagac cttgcccgag cccatcatca cgctgatcac
     1021 gttgagcatc gggaagttct cgcagacgat cttgatgatc gcggcgatga tcaccgagat
     1081 gagcgcgccg accacgcccc agatgagtcc ccagaagagc agcgagatga ggaccactac
     1141 cgggctgagg ttgagccggt cgcccatgac cttcggcgtg atgaagttgc cgatgaccac
     1201 ctgaatggtg agcagcgtga gcgcggtgat caggggggct gtcaggctcg ggtagaactg
     1261 gactacggcc acgaggatcg gaggaatgga cgcgacgatc gagccgatcg tggggatgaa
     1321 gttgaggatg aaagcgagca ctccccacgt tacagcgaaa tccacctgca ggtattcaag
     1381 ggacatccag acgatcagcc cggtcaccgc gctgatgagc gccaaagcgc tgaggtaacg
     1441 ccctatctgc cacgatatgg tgctcagaat cttgcgtgca cgttcggagc cgtggggcgt
     1501 gacaaaggct ttcttcacct tgtattcgaa atacggagag ccgagcagca taaagacgag
     1561 gaagacgatg accataacaa gtttcgagat gatggtgacg aaggagctgg agagatcgag
     1621 gagatacccc ctgatggtga acccccagtt gatggacgtc cagaagtcag gaggaatatc
     1681 gagattcgtc gtgagcgact tgcctatctc catcaggcgc gagtagtact tcggaaaggc
     1741 ggccgcgaac gaggcgattc gggtattcag aaagatagcg ccgaggaagc agagaccgaa
     1801 aaaaatggca aggacaaaaa ccaccgcaag ccccacagga acgcggcgtt tggcgaggta
     1861 acgaacgatg ggaccaaaaa gatacgaaag aagccatgca ataataagcg gaatgaagac
     1921 cgcctgtgcg aagttcagca caaaaccaac ggcgacgaga gcgatcactc ccacgaggcc
     1981 gatcataacc tgaaattttt ccacactatc ccttcccttc cttgagatgc gagcagaccc
     2041 ctccacactt attattctac actgaggaat ccgttttggg agaaaagggt gaataaaaaa
     2101 tacatcgcgc tgaaaaaaga aggaaaac
//
LOCUS       DAOSWW010000470         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42220_length_2126_cov_4.232255, whole genome shotgun sequence.
ACCESSION   DAOSWW010000470 DAOSWW010000000
VERSION     DAOSWW010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42220_length_2126_cov_4.232255"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..1481)
                     /locus_tag="PK442_09320"
     CDS             complement(267..1481)
                     /locus_tag="PK442_09320"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOO87893.1"
                     /translation="MEEMYARCCGIDVHKKKFTACYREGGKNEIREYETTSEGIRGFV
                     GWLKERGAQISAMESTGVYWKSLYNIMEENGLEVIVVNAGHMKAVPGRKTDKNDAEWI
                     AELLACGLLKASYIPDREQREMREITRYRKSLAEERARELNRLQKVLEGANIKLSCVV
                     SDINGQSARRIIGALVGEKGNPKREEVVDLLDERMKPKADEIMKAIDGYVTPMQKQLI
                     RAITAHIDDMSKRIKELDKLIDDEMNKHRNNTRRLDEIPGIGKRTAETILSEIGSDMQ
                     RFPTEAHLSSWSGLSPGNNESAGKRHSGKTNKGNRTLKTALIQAARAAVHGDNYFRAQ
                     YERLLVKRGKNRAVVAVAHSMLIVIYHMLKDGTRYQELGSDYYAKRDPRKEIARLQKK
                     MDYLNTLIQTPV"
     gene            complement(1702..>2126)
                     /locus_tag="PK442_09325"
     CDS             complement(1702..>2126)
                     /locus_tag="PK442_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HOO87894.1"
                     /translation="SDKQKDEAARLFMAGETDLLCLSLRAASGLNLQRATCVVFGELD
                     WSPAVHSQAEDRAHRIGQRDSLLCYYLVSPRGSDRDMQDALGLKVSQFVSIMGDSESD
                     RDTRDLLQSEARARIRALVERLAGEEDAGAPPQTPLKG"
BASE COUNT          345 a          680 c          558 g          543 t
ORIGIN      
        1 gaatcccgtt aagggtaaaa tttcatttgt tccgtcggat cgcgcggggg cccgaacccc
       61 gcgcgatccg cgtttatggt gcggggaggc gcgcggggct gcggggtttt cctaaaacca
      121 aagctcggcg gcgcggggtt gggcccccgc gccgtcgacg gacagtcttt cattttacac
      181 ttagaatggg attgctatga aactctccgc actttaaggc ataagaatgc acggctccct
      241 tatcaggggg ccattcagag ggaatatcag acgggagtct gaatgagggt gttcaggtag
      301 tccatctttt tctggagtcg tgcgatttcc tttcgcgggt cgcgctttgc gtagtagtcg
      361 ctccccagtt cctggtatcg cgtgccatcc ttgagcatgt ggtagatcac aatcagcata
      421 gagtgcgcaa cagcgaccac cgcgcggttt ttcccgcgct tcacaagcag ccgttcatac
      481 tgagcgcgga agtagttgtc cccatgtacc gcggctctcg cagcttggat cagcgcggtc
      541 ttcagcgttc ggttcccttt gtttgtcttc ccactgtgcc gctttcccgc gctttcgtta
      601 ttcccggggc tcagtccgct ccacgatgac aggtgcgcct ctgtcggaaa tctttgcata
      661 tcactgccga tctctgacag aatcgtctcg gccgtccgct tcccgattcc cggaatttca
      721 tccagccgac gcgtattgtt tctgtgcttg ttcatctcat cgtcgatgag cttatccaac
      781 tccttgatcc gtttgctcat gtcgtctatg tgcgcggtga tcgcgcggat cagttgcttc
      841 tgcatcggcg tcacgtatcc gtctatcgcc ttcatgatct cgtccgcctt cggcttcatc
      901 cgctcatcca gaaggtccac gacctcctcg cgcttggggt tccctttttc tcccacaagc
      961 gctccgatga tccgtcgcgc gctctggccg ttgatgtcgc ttaccacaca actcaacttg
     1021 atgttcgcgc cttccagtac cttctgcagc cggttcagtt cccgcgctct ctcctccgcc
     1081 aggctcttgc gataccgcgt gatctcccgc atctcccgtt gctcccgatc cggtatgtag
     1141 ctcgccttca acagtccaca tgccaacagc tccgcgatcc actccgcatc gttcttatct
     1201 gtcttgcgac ccggtaccgc cttcatgtgc cccgcgttca cgacgatgac ttccagaccg
     1261 ttctcttcca tgatgttgta cagggacttc cagtataccc ccgtgctctc catggcagaa
     1321 atctgcgcgc cacgctcctt cagccaccct acgaatcctc gaatcccttc ggatgtcgtt
     1381 tcatattccc gtatctcgtt ttttcctccc tcccgatagc atgccgtaaa cttcttctta
     1441 tgcacgtcga ttccacaaca cctcgcgtac atctcctcca tggctctttc cccttccttg
     1501 gagccgggcg tacctatctt tgtcagattg ctatgcatct tcgtatgata tcttgtggtt
     1561 caaccaggta cacccactca tattctcaat cgtcctctca cggacaaatc ccagcgagct
     1621 tgcgctctac tgggattata ccgcttctat tcagtttcgt acatcgtggt gtaccttggt
     1681 tcgtggctat tctcaagggc gttagccctt gagcggggtc tggggtggag ccccagcgtc
     1741 ctcctccccc gccagtcgct ccaccagcgc ccggatgcgc gcccgggcct cggactggag
     1801 caggtcgcgg gtatcgcggt cggattcgga atctcccatg atggagacga actggctgac
     1861 cttgaggccg agggcgtcct gcatgtcgcg gtcggagccc cggggggaga cgaggtaata
     1921 gcagagcagg gaatcgcgct ggccgatgcg gtgggcgcgg tcctcggcct gggagtggac
     1981 ggcgggggac cagtcgagct cgccgaacac gacgcaggtg gcgcgctgga ggtttaaacc
     2041 gctcgccgcg cgcagggaaa ggcataaaag gtccgtctcc cccgccatga acagccgcgc
     2101 cgcctcgtcc ttctgcttgt cggact
//
LOCUS       DAOSWW010000471         2125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42263_length_2125_cov_3.219807, whole genome shotgun sequence.
ACCESSION   DAOSWW010000471 DAOSWW010000000
VERSION     DAOSWW010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42263_length_2125_cov_3.219807"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PK442_09330"
     CDS             <1..673
                     /locus_tag="PK442_09330"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOO87895.1"
                     /translation="SSLHVRLGALVVQAYMKRGETAEQALELVRSSPLVLSVRAISPE
                     EALERLRAKLGNQAQAVTLLGENPLPWSLEIQVQRAEHITPLVRDLMVLPSIEEVLYA
                     GKLVERLATISRASSAVSGIILMLTLLISTLVIFNTIRIAIYSRKEEIEVMLLIGATS
                     TYISLPFVIQEMLLGAGGAILAIFGLWSTYSSAIEAIRSTLAFVDIIDNRRILAQFYV
                     FLFAA"
     gene            772..1971
                     /locus_tag="PK442_09335"
     CDS             772..1971
                     /locus_tag="PK442_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOO87896.1"
                     /translation="MKKTTVLSVLLLAAFISLFYSAPYCEAASDIDSKLVQEEARMRT
                     LERQISEHQQRVRQMGEREQGVISRIEELDQKRKMTEQRIRVLELRSAKTKQAVEDLR
                     REIQATEQSLVSMKRVLEDRLVNIYKYGGVAELNLLLSASTAHEAMETTLLLNRIALQ
                     DETMITDMLVKKERLGEAAAQLEAERNQLAINSAALEKSRKTYRQEIASSDSFLQKVR
                     SERALHQRAVKELQDSQREIQSTIMDLMRKKREAEMRPDAPPVTREEVPSGGKLAWPL
                     PSKGRITSSFGMRVHPTFKTRMMHTGIDIQMPSGTPVYAAGPGEVLYAGWLRGYGQIV
                     IIDHGRDLSSVYAHLSRLAVGEGDRVKKGQNIGAVGTTGTTTGAHLHFEVRVNGEAKD
                     PMKYLGK"
BASE COUNT          452 a          584 c          654 g          435 t
ORIGIN      
        1 ctcctcgctt catgtacgcc tgggcgccct cgtcgtccag gcgtacatga agcgaggaga
       61 gacggcggag caggcgctcg agcttgtccg ttcgtcccct ctggttttgt ccgttcgcgc
      121 catctccccg gaggaggcgc tcgaaagact gcgtgcaaag ctgggcaatc aggcgcaggc
      181 ggtcacgctg cttggagaga atcctcttcc gtggagtctg gaaatccagg tgcagagggc
      241 ggagcacatc acgccgcttg tccgcgacct tatggtgctt ccgtcgatcg aggaggtgct
      301 ctacgccggg aaacttgtgg agcgtctcgc cacgatctcc cgggcatcaa gcgcggtatc
      361 gggaatcatt ctcatgctga ccctgctgat aagtacgctc gttattttca atacaattcg
      421 catcgccatc tactcccgga aagaagagat cgaagtgatg cttctcatag gagcgacgtc
      481 gacctatatc tcgcttccgt tcgtcatcca ggagatgctt ctcggcgcgg gcggtgcgat
      541 attggctatc ttcggtttgt ggagcaccta ttcttccgcc attgaagcta tccgttcgac
      601 gctcgcgttt gttgatataa ttgataaccg gcgcattctg gcccagtttt acgttttcct
      661 tttcgccgcc tgagccacta cgggctggat gtgcagctgg cttgccgtgc atcgtttcac
      721 atctcagagt atgcgttccc ggtagtcctg tccgtgaagg aggttggcgg tatgaaaaaa
      781 acgacggttc tctccgtact tctgctcgca gcgttcattt ccctgttcta ttcggcgccg
      841 tattgcgagg ctgcctccga tatcgactcg aaactcgttc aggaggaggc gcggatgcgg
      901 acgctcgaac ggcagatatc cgaacatcag cagcgtgtcc gccagatggg ggagcgggag
      961 cagggcgtta tctcccggat agaggagctt gatcagaagc ggaagatgac ggaacagcgc
     1021 attcgcgtgc tcgaactgag gagcgcgaaa acaaaacagg cggtcgagga tctgcgccgc
     1081 gagattcagg cgaccgaaca gtctctggtc tcgatgaagc gcgtgcttga agatcgcctg
     1141 gtgaatatct ataaatacgg cggcgttgcc gagctcaatc tgctgctctc ggcttccacg
     1201 gcccacgaag caatggagac gacgctgctc ttgaaccgca tcgctcttca ggacgaaacg
     1261 atgatcaccg atatgctcgt gaaaaaagag cgtctcggcg aggcggccgc gcagcttgag
     1321 gcggagcgga atcagctcgc gatcaactcc gctgctctcg aaaagagcag gaagacttac
     1381 cggcaggaga ttgcgagcag cgacagcttc cttcaaaaag tccggagcga gcgcgccctc
     1441 catcagcggg ccgtcaagga gcttcaggac tctcagcggg agattcagtc caccatcatg
     1501 gatctgatgc ggaagaagcg cgaggcggag atgcgccccg acgcgcctcc ggttactcgc
     1561 gaagaggtcc cgtcaggagg gaaacttgcc tggccgctcc cctcgaaggg acggatcacc
     1621 agttcttttg gtatgcgagt gcacccgacc ttcaagacgc gaatgatgca caccggaatc
     1681 gatatacaaa tgccaagcgg aactccggtc tacgcggcgg gtccaggaga ggtcctctac
     1741 gcgggatggc ttcgcggcta cggccagatc gtcatcatcg atcacggcag ggatctctcc
     1801 agcgtgtacg cgcacctttc gcgccttgcg gtcggcgaag gggaccgtgt gaaaaaaggg
     1861 cagaatatcg gcgcagtcgg cactaccggg acgacgacgg gagcgcatct tcatttcgag
     1921 gtcagagtca acggcgaagc caaggatccg atgaagtatc tcgggaaata aaccgtaaaa
     1981 caaaggagaa gaagaatgca taaggaacta tcgccgctct ggaggcgggt acgaggctgg
     2041 atcgccggaa tggtcgtcgg cgttgtcgtt ggagtcgccg tgacgggagg cttgctcgcc
     2101 gcaggagcga tggatctggg cgagt
//
LOCUS       DAOSWW010000472         2122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42364_length_2122_cov_3.010160, whole genome shotgun sequence.
ACCESSION   DAOSWW010000472 DAOSWW010000000
VERSION     DAOSWW010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42364_length_2122_cov_3.010160"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PK442_09340"
     CDS             <1..754
                     /locus_tag="PK442_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOO87897.1"
                     /translation="DFVVIRENTEDFYVGIGGKTEKGKTVVDLATTRGLYSMSGTLEG
                     AFSDGVEGAFQLGVASEPGVRRVVAYAARAAKARGEEKITLASKSNALPQIYGFWEDV
                     AKDEAAKQGVGLSIVNVDAMCYHLVRTPDLYGVVVAPNMFGDIVSDLLAGLAGGLGVA
                     AGADIGDQLSMFEPIHGSAPDIAGTGKANPIAAILTGALMLEHIGERDAAQGVENALT
                     EFLAHAETRDLPVEFGGEATTSAVGEAIAKRV"
     gene            complement(1035..1796)
                     /locus_tag="PK442_09345"
     CDS             complement(1035..1796)
                     /locus_tag="PK442_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOO87898.1"
                     /translation="MVNELKRKLARGEVALGTFITLNCPDLVEIAGLAGFDFCIIDTE
                     HGPGDAESIQHMIRAAELRGITPVVRVTNPEATTILRILDVGAAGIQAPQVNSRKTAE
                     DVVRFAKYFPKGERGACLTRSSRYGFVEGVAQYFEEANRETMVILHCENKQGLECLDD
                     IASVEGVDVVFVGPYDLSQSFGIPGQIYHPVMVEAVAKALAVAKRAGKPAGIFVSSVE
                     EAKARIEQGFAYVAYNTDSLLFAELCRNIVKGVKG"
     gene            complement(1904..>2122)
                     /locus_tag="PK442_09350"
     CDS             complement(1904..>2122)
                     /locus_tag="PK442_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyvinyl-carboxyphosphonate
                     phosphorylmutase"
                     /protein_id="HOO87899.1"
                     /translation="LSTLYSAARGMYDVMKRIHAEGVSDGYLDKLFTFKEFGELVRLD
                     EVRAKEEKYLPPSVMQDKYRGKKHIVEK"
BASE COUNT          384 a          692 c          659 g          387 t
ORIGIN      
        1 cgacttcgtg gtcatacggg aaaacacgga ggacttctac gtcggcatcg gcggtaaaac
       61 ggaaaagggg aagaccgtgg tcgatcttgc gacgacgcga gggctctact ccatgagcgg
      121 aacgctcgaa ggcgcgttct cggacggcgt ggagggcgcg ttccagctcg gcgtggcctc
      181 ggaaccgggc gtccgccgcg tggtcgcgta cgccgcgcgc gccgcgaagg cccgaggcga
      241 agagaagatc acgctggcct cgaaatcgaa cgccctgccg cagatctacg gcttctggga
      301 ggacgtcgcg aaggacgagg cggcgaaaca gggcgtcggg ctctccatcg tgaacgtgga
      361 cgccatgtgc taccacctgg tgcgcacgcc cgacctctac ggcgtcgtgg tcgccccgaa
      421 catgttcggc gacatcgtca gcgaccttct cgcagggctc gccggagggc tcggcgtcgc
      481 cgccggagcc gacatcggcg accagctctc tatgttcgaa ccgatccacg gctccgcccc
      541 cgacatcgcg ggaacgggga aggcgaaccc catagcggcc atcctcaccg gggcgctcat
      601 gctggagcac atcggcgagc gtgacgccgc gcagggcgtc gagaacgcgc tgacggagtt
      661 cctggcgcac gccgaaacgc gcgacctccc cgtcgagttc ggcggcgagg cgaccacctc
      721 cgccgtggga gaagccatcg cgaaacgggt gtaaactatc gaaaagcgcc gctcccgtcg
      781 acacggagcg gcgctttttg tcgattctct cctcgtaaga aacagtgtgc ggcggaagag
      841 cgaagaggag cggaccaacg caggctccgc atccgataaa gagggcgcga cgaattcgta
      901 caggaaggag aggctgcagc gctccttggc gtccggtatc cttgtacgtt ttttctgcgc
      961 atacgcgttt cttcagagcg ggaacgctct tccgccgtac cgcctgtccc gattcaggag
     1021 cgcgttatct gctcctaccc cttgacgccc ttcacgatgt tccggcagag ctcggcgaag
     1081 agcagcgagt cggtattgta ggcgacgtag gcgaacccct gctcgattct cgccttcgcc
     1141 tcctcgacgg aggagacaaa gataccggcg ggcttcccgg cgcgtttggc gactgcgagc
     1201 gccttcgcta cggcttcgac catcacgggg tggtagatct gccccggaat gccgaaggac
     1261 tgcgaaaggt cgtacggtcc cacgaagacc acgtccaccc cctccacgga agcgatgtcg
     1321 tcgagacact ccagcccctg cttgttctcg cagtggagga tcaccatcgt ctcgcggttg
     1381 gcctcctcga agtactgcgc caccccctcc acaaatccgt accgggacga ccgcgtaaga
     1441 catgcgcctc gctccccctt cgggaagtac ttcgcgaagc ggaccacgtc ctccgccgtc
     1501 ttccgcgaat tcacctgcgg ggcctgaatt ccggcggcgc ccacgtcgag gatgcggaga
     1561 atcgtggtgg cctccgggtt cgtcaccctg acgaccggcg tgatcccccg aagttcggcg
     1621 gcgcggatca tgtgctggat ggactccgcg tcgccggggc cgtgctcggt gtcgatgatg
     1681 cagaagtcga accccgcgag ccccgcgatc tcgaccagat cggggcagtt cagcgtgatg
     1741 aacgtgccga gagcgacttc gccccgggca agctttcttt tcagttcatt gaccattcaa
     1801 gtatccctcc gtcgactgca ttctgcgttt ccgatacata ttataccctc gaaaaagggg
     1861 cggaggtttt ccccccgccc ctgcgtttcg acaaacttcc cggtcacttt tcgacgatgt
     1921 gcttcttccc cctgtacttg tcctgcatca cgctcggggg gaggtacttc tcctccttcg
     1981 cgcggacctc gtcgaggcgg acaagctcgc cgaactcctt gaacgtgaag agcttgtcga
     2041 gatagccgtc ggagactccc tcggcgtgaa tcctcttcat gacgtcgtac atgcctcggg
     2101 ctgcggagta gagcgtcgac ag
//
LOCUS       DAOSWW010000473         2112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42673_length_2112_cov_3.785124, whole genome shotgun sequence.
ACCESSION   DAOSWW010000473 DAOSWW010000000
VERSION     DAOSWW010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42673_length_2112_cov_3.785124"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(423..1358)
                     /locus_tag="PK442_09355"
     CDS             complement(423..1358)
                     /locus_tag="PK442_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOO87900.1"
                     /translation="MHGFFVILPVVLVMAAGWMLGKRRVVLPEAFAQINKAIYWVAIP
                     ALILRMTSSADMSTLADKNMVLAVYASFLLAPPFAWFAARMAGEKREKKATSTLMLIR
                     SNTVFMGLPVVTIAVGMKGVEVLSLYLAFTFIGYQIISISWAQLALSGGISRSTVKET
                     LKNLAKNPLVVSSLAGFGLSVFGWNTFPAWLDETLKLLGNTASGMALLSLGASLKLDG
                     LSGILPRVWRDATLKLLFLPALTWCFFLLLPVDPVVFRTVLLISAMPVAVDCFILSQV
                     LGMDETHAAETISASTVLSSLTVPFWITMMDLFAR"
     gene            complement(1363..>2112)
                     /locus_tag="PK442_09360"
     CDS             complement(1363..>2112)
                     /locus_tag="PK442_09360"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOO87901.1"
                     /translation="SVSREFAPIAAEAGSVVIDNSSAFRMDSAIPLVVPEINGDLLKG
                     YTQGIVANPNCSTIQMVLGLYRIHERFGIRSIVVSTYQSVSGAGKNGMNELEEQMKGT
                     IAPKKFIKQIHLNVVPQIGALLENGFTDEEMKMVNETRKILRDPDISIWATTIRVPVL
                     YCHSESIYVETRTAFDMDGLAEALEASENVIHTDEMFSPIDVAGSDTVYVGRLRAFDA
                     TRFLMWNVADNIRVGAATNAVRILLKHRALN"
BASE COUNT          508 a          580 c          586 g          438 t
ORIGIN      
        1 gaatctatca ttgcatccct cgggggatac tcttatttcc taaatagtac agcataatag
       61 ccttttaaag aggttctccg gaaatttcca ctctgcggta acacttgcca tttagtggat
      121 ttattctata tatatcagac aaagagagcg gtgtcaaggg tttttctgat gatggaaaaa
      181 agggggacct ccctggattg ttctcaaaca gagggaatgt gttttcaagc aacccgctgt
      241 tcgatggtgt ttcatgctac agaatgccgc aaggagcaaa tcgtgccggc tcgactactc
      301 ccggatcttg ccgggcagat aaaagaaccg cccttcacga aacagaagga cggttcttta
      361 tgcaaaaagt tacaaccata catttcatcc atattaaaaa aaagtcttac attacccctt
      421 gtctaacggg caaaaagatc catcatcgta atccaaaagg gcacagtgag gctcgaaagt
      481 acggtactcg ccgagatagt ctccgcggcg tgcgtttcgt ccatgcccag aacctgcgag
      541 aggatgaagc agtccaccgc gacaggcatg gccgaaatga gaaggaccgt ccggaagacg
      601 accggatcga ccggcagcag aaggaagaaa caccaggtga gcgccgggag aaagaggagc
      661 ttcagcgttg cgtcgcgcca gacgcggggc agtattcccg aaagcccgtc gagcttgagc
      721 gacgcgccga gcgagagcag cgccatgccg cttgccgtgt tgccgagcag cttgagcgtt
      781 tcgtccagcc aggccggaaa ggtgttccac ccgaaaaccg agagcccgaa cccggcgagg
      841 ctggagacga caagcgggtt cttcgccagg ttcttcagcg tctctttcac cgtgctgcgc
      901 gaaataccgc cggagagcgc aagctgggcc caggagatag agatgatctg gtacccgatg
      961 aacgtaaacg cgaggtagag cgagagcacc tctactccct tcatacccac tgcgatcgtg
     1021 acgacgggca gccccataaa aacggtattc gaacgaatga gcatcagcgt ggacgtcgct
     1081 ttcttctctc gtttttcccc tgccatccgg gccgcgaacc atgcaaacgg aggcgccaaa
     1141 agaaaggatg catagaccgc aagcaccatg ttcttgtcgg caagcgtcga catgtccgcg
     1201 ctgctggtca ttcgcagaat gagcgcgggt atggcgaccc agtagatagc cttgttgatc
     1261 tgcgcaaagg cttcggggag aacgacacgc cgtttgccga gcatccaccc ggcggccatg
     1321 acaagcacca ccggaaggat gacaaaaaaa ccgtgcatac cgctagttca aagcgcggtg
     1381 tttcaacagg atgcgaacgg cgttcgttgc ggctccgacg cggatgttgt ccgcgacgtt
     1441 ccacatcagg aagcgggtgg cgtcgaacgc ccgaaggcgc ccgacataga cggtatcgct
     1501 tccggcgacg tctataggag agaacatctc atccgtatgg atgacgtttt ccgacgcttc
     1561 gagcgcttcc gcaaggccgt ccatatcgaa cgctgtcctc gtctccacat agatcgactc
     1621 ggagtggcaa tagagcacag gcacccggat cgttgttgcc cagattgaaa tgtcagggtc
     1681 tcgcaggatc ttcctcgtct cgttgaccat cttcatctct tcgtcggtga aaccgttctc
     1741 cagcaacgcg cctatttgcg gcacgacgtt gaggtgaatc tgcttgatga acttcttcgg
     1801 cgctatagtc cctttcatct gttcttcaag ctcgttcata ccgttcttcc ctgctccgga
     1861 gacggactgg taggtgctca ccacgatgga gcggatgccg aaacgctcgt gaatcctgta
     1921 gagccccagc accatctgga tggtcgagca gttcgggttt gcaacgatcc cctgcgtata
     1981 gcctttgagc agatccccgt tgatctcggg gacgacgagg ggaattgcgg aatccatccg
     2041 gaacgcagag gagttgtcga tgaccacgct tcccgcctcg gcggcgatgg gagcaaattc
     2101 acgggagacg ga
//
LOCUS       DAOSWW010000474         2110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42770_length_2110_cov_1.527981, whole genome shotgun sequence.
ACCESSION   DAOSWW010000474 DAOSWW010000000
VERSION     DAOSWW010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42770_length_2110_cov_1.527981"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PK442_09365"
     CDS             <1..224
                     /locus_tag="PK442_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87902.1"
                     /translation="GGISGIGGVLMTVSILVGIIGGFIRASNAGQAFLYRGLGAVGEV
                     GSFGVFLVAGMGVAGLVLSGFLQSMPGKK"
     gene            complement(333..1124)
                     /locus_tag="PK442_09370"
     CDS             complement(333..1124)
                     /locus_tag="PK442_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOO87903.1"
                     /translation="MKTREAFPSVLLIGQFAKLSGLSSRMLRFYAERNILVPAFTDNA
                     TGYRYYLPSQLIEAKLLRRLRQADFSLDDIAVILNGLETAQWGECVAAQRESITRKIA
                     AMKMALEELEHLEKHRGPEEYVLARFAPMRVLSAPLAGERLRLREIARTEAKRLFGED
                     GANAPLFCLYDLHEGPARSTICIPAEEPCGEIMPECVLAVSLHRGAYETVPATLEQLL
                     TWVKQNEFRTNGRVLERYFPSGEEDHPHGRVEIAVTLEGTPLPTA"
     gene            complement(1121..1393)
                     /locus_tag="PK442_09375"
     CDS             complement(1121..1393)
                     /locus_tag="PK442_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87904.1"
                     /translation="MMDEFGRSLRSFGLDPERVLSALRDGDVSAVLASLKKEEDMLLL
                     RNIELADLREQLLAMAGEEGRRKNDELSDDDVDLLSAAGQEPRSIP"
     gene            1862..>2110
                     /locus_tag="PK442_09380"
     CDS             1862..>2110
                     /locus_tag="PK442_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HOO87905.1"
                     /translation="MSMESAAAFYERLQSDAALQENLKAAGNRDQVEAYVKGELGYDF
                     TKEEMQKVVFERNPEMTDEELEAVVGGASNDMMWGITIG"
BASE COUNT          442 a          575 c          616 g          477 t
ORIGIN      
        1 caggcggcat ttccggaatc ggcggcgtcc tcatgacagt cagtattctc gtcggcataa
       61 tcggtggttt cataagggct tcaaatgcgg gacaggcgtt cttgtacagg ggactcggcg
      121 ccgtcggcga agtcgggtca ttcggtgtgt ttctggtcgc cggaatgggc gttgcggggc
      181 tggttctttc gggctttctt cagtccatgc cgggaaaaaa gtagaaaaga agtcttcttt
      241 attcgtctgc tgcgggaacg gcgcatgcca taggggagcg gttccttccc gctgtgctgg
      301 aaggaaccgc tccgtcgatg gatatgcccc gttcaggccg tgggaagagg tgttccctcc
      361 agggtgacgg cgatctccac ccttccgtgg gggtgatctt cctccccgct tggaaagtac
      421 cgttcgagca cgcgcccgtt tgttctgaat tcgttttgtt tcacccacgt tagaagctgc
      481 tccagagtgg ccggaaccgt ctcgtacgct cctctgtgga gcgagacggc aagcacgcat
      541 tccggcataa tctccccgca gggctcctcc gccggaatgc agatcgtcga tctggccggt
      601 ccctcgtgca gatcgtagag gcagaaaaga ggcgcgttcg cgccgtcttc tccgaagagg
      661 cgtttagctt cggtacgcgc gatctcgcga agccgcagtc tttcgccggc cagaggggcg
      721 gagagaacgc gcatcggggc gaaacgggcc agcacatact cctccggccc ccggtgcttt
      781 tcgaggtgct ccagctcctc cagcgccatc ttcattgccg ctatcttccg ggtgatcgac
      841 tcgcgctggg cagcaacgca ctctccccac tgcgccgtct ccagtccatt gagaatcacg
      901 gcgatgtcgt cgagtgaaaa gtcggcctgc cgcaaccgtc gcagcagctt tgcttcgatg
      961 agctgcgacg gcagatagta ccgatacccc gttgcattgt cggtgaacgc gggaacaagg
     1021 atattccgtt ccgcataaaa acgaagcatc cggctcgata ggccgctcag cttcgcgaac
     1081 tgccctatga gcagtacgga cggaaacgct tctctcgtct tcatgggatg cttctcggtt
     1141 cctggcctgc cgcgctcagc agatcgacat cgtcatcgga aagttcatcg ttctttcttc
     1201 gtccctcttc tccggccatg gcgagcagct gctcgcggag gtcggctaac tcgatgttcc
     1261 gcagaaggag catatcctcc tcctttttca gggaggccag aacagcgctg acatctccgt
     1321 cccgcaaggc gctgaggacc cgttccgggt ccagtccgaa cgaacgcagc gaccggccga
     1381 actcatccat catttccccc cccctttggg tacccgattg aaaacggcgt catcgtacac
     1441 cttcacacga tgtgagagtc aatcctcccg gatttcttcg atggtccgcc gacatcggat
     1501 gggtgccgtc aaagtacaca aagtatgcca tgaagctttt tctccttcaa gcaatccagg
     1561 aacaaatttt taattttcgc acttgacatt tacacgatgt caatgtctat actgtccgca
     1621 gaagagattt tttcgttttt gtatatcttg gaggattgaa catttctgcg gaatctgcgg
     1681 cagcgttcta cgggcttctg gaaaaaaatc ggcacgactt cacgctggag gagatgcaga
     1741 aagtgatctt cgggaagaat ccggagatga ccgacgagga acttgaggct gccgcagaaa
     1801 ttgcaggtgt gtatataacc tcttccgaaa agggagagag tataaaagga ggtcgtatta
     1861 tatgtcgatg gaatctgcag cggcgttcta tgagcgtctc cagagtgacg cggcgcttca
     1921 ggaaaacctg aaggcggcgg ggaacaggga tcaggtggaa gcgtacgtga agggggaact
     1981 tggttatgac ttcacaaagg aagagatgca gaaggtggtt ttcgaacgga acccggagat
     2041 gaccgacgaa gagctggagg ctgtcgtcgg aggagcaagc aacgatatga tgtgggggat
     2101 caccatcggc
//
LOCUS       DAOSWW010000475         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_42799_length_2109_cov_2.954722, whole genome shotgun sequence.
ACCESSION   DAOSWW010000475 DAOSWW010000000
VERSION     DAOSWW010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42799_length_2109_cov_2.954722"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PK442_09385"
     CDS             complement(<1..728)
                     /locus_tag="PK442_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOO87906.1"
                     /translation="MSVRVSDEMTKVRARAKRRLVIKRALTYLTAIVLSLWILLPIWL
                     IASMALTTPEAMRNFPKGVLPFIPFSLDTMRFFLEARGIIPGLINSVVVALITLALST
                     LIAAPAGYALSRYVFRGRNAFRLSILAVRAFPVVILSIPLAVTFINLGMFDKVSSLAL
                     MHTALTLPTTVLVIGSVFASIPYELEEAAQVFGCTAFQAFRRVVLPLVLPGIAAAALF
                     TFVMSWNEVFAATVLTVQNRTLPA"
     gene            complement(725..1894)
                     /locus_tag="PK442_09390"
     CDS             complement(725..1894)
                     /locus_tag="PK442_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOO87907.1"
                     /translation="MIDRRNVPLAPGEADGGPALLDVWFFVEGETPGGATVQGWAPES
                     RVRVRETDPSGVPLRGTVRPRLGNTAGQHTTLHASPDERSDVVGELASRAEIGIAQRT
                     SLEVWFLIEGVRNEQTLSGWAPSRRLQIYGDGATGRVDSGDTGRFTLLHIQRMLNDRF
                     FRSALGTTFLLMLLIIPVQLVLAIVMALVIQARLKFNLLFLAIFAIPLAASDLAVGIV
                     WSSIFTQNGYLNSILYSLGLIESPQAYLTAATRHWILVAVWIAEVWRATSLVMVIVVS
                     GLQAISQEVLEAAELFGATLWQRVRYVILPLLKPSLQVALILRTILALQVFAVVIALG
                     GGDVVTVLANETYRQYYALRNMNVAAAYSGLILLLSMISAVIYLRTVRGQEEVVS"
BASE COUNT          432 a          633 c          717 g          327 t
ORIGIN      
        1 tgcgcgggca gcgtgcgatt ctgcaccgtg agcactgtgg ccgcgaagac ttcgttccag
       61 gacatcacga aggtgaagag cgcggcggcg gcgatgccgg gcagcaccag cggcagcacg
      121 acccgccgga atgcctggaa ggccgtgcag ccgaagacct gtgccgcctc ctcgagttca
      181 tacggaatgc tcgcgaagac gctcccgatg acgagcaccg tggtaggcag cgtgagcgcc
      241 gtgtgcatca gcgcaagact tgagaccttg tcgaacattc ccaggttgat gaaggtcacc
      301 gcaagcggaa tggagaggat gaccaccgga aaggcgcgca cggcgaggat ggagaggcgg
      361 aatgcgttac ggcctcggaa gacgtagcgc gagagcgcgt atcctgccgg cgccgcgatc
      421 agcgtggaga gggccagggt gatcagcgcg acgacgacgc tgttgataag accggggatg
      481 ataccgcgcg cctccaggaa gaagcgcatg gtgtccaagg agaacggaat gaagggcagc
      541 acgcccttgg gaaagtttcg catcgcttcc ggcgtggtga gggccatcga cgcgatgagc
      601 caaatcggaa gaagaatcca cagagagaga acaatcgccg tcagataggt gagcgcccgc
      661 ttgatcacga ggcggcgctt cgcgcgagcg cgaaccttcg tcatctcgtc cgaaacccgt
      721 acgctcatga gaccacctcc tcctgtccgc gtacagtacg cagatagata acggcgctga
      781 tcatcgaaag caggagaatc aggcctgaat atgcggcggc cacgttcatg ttgcggaggg
      841 cgtagtactg gcggtaggtc tcgttggcga gcaccgtgac cacgtcgcct ccgccgagcg
      901 cgatcaccac ggcgaagacc tgcagcgcga ggatggttct caggatgagg gcgacctgaa
      961 ggcttggctt gagcagcgga agaatcacgt agcgcacccg ctgccacagg gtcgcgccga
     1021 agagttcggc tgcctccagc acctcctgcg agatcgcctg cagcccggag acgacgatca
     1081 ccatcacgag ggacgtcgcc cgccagacct cggcgatcca gaccgcgacc aggatccagt
     1141 ggcgcgtcgc ggcggtgagg tatgcctgcg ggctttcgat cagcccgagg gagtagagaa
     1201 tgctgttcag atagccgttc tgcgtgaaga tggaggacca gacgatcccc acggcaaggt
     1261 ccgacgcggc cagcgggatg gcgaagatgg cgaggaagag cagattgaac ttcagccgcg
     1321 cctgaatcac cagcgccatc acgatcgcca gcacgagctg cacagggatg atcagcagca
     1381 tcaacaggaa ggttgttccc agcgcggagc ggaagaaacg gtcgttgagc atcctctgaa
     1441 tgtggagcag ggtgaagcgg ccggtatcgc cggagtccac ccttccggtc gcgccgtcgc
     1501 cgtagatctg cagcctgcgg gagggcgccc atccggagag cgtctgttca ttgcgtactc
     1561 cttcaataag aaaccagacc tcaagagatg tccgttgcgc aataccgatc tccgctctgc
     1621 tcgcaagctc cccgacgacg tcgctccgtt cgtcggggga agcgtgcagc gtcgtgtgct
     1681 gtccggcggt gtttccgagg cgggggcgaa ccgtgccccg gagcgggacg cccgagggat
     1741 cagtttcgcg gacgcggacg cggctttcgg gcgcccaccc ttggaccgtg gctccgccgg
     1801 gggtctcgcc ctcaacaaaa aaccagacgt cgaggagggc ggggcctccg tccgcttcgc
     1861 cgggggcaag agggacgttc cggcggtcga tcacggttat ctgcgccccc tgttcgagcg
     1921 cgcccgcctt attcagctct ccggcgccgc gcgcagcgtg aggagaccct gttcgtccca
     1981 gaccgccagg cgaattgcct gcgtcatcgg ccaggcgaag aagaccagca gatagagcag
     2041 ggcgggcgta atcaagaggt acggcgccca ctgcactttc gtgcggcgcc ttggtgttcc
     2101 gagaggctg
//
LOCUS       DAOSWW010000476         2101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43080_length_2101_cov_2.864125, whole genome shotgun sequence.
ACCESSION   DAOSWW010000476 DAOSWW010000000
VERSION     DAOSWW010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43080_length_2101_cov_2.864125"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK442_09395"
     CDS             <1..467
                     /locus_tag="PK442_09395"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HOO87908.1"
                     /translation="STVLDATGEQLVLLRPGGCAVEEIEARSGMKVLLSGGAAHRSPG
                     TRYRHYAPRVPLLLDTDADGIARAAAEADGALRIAWIGMHAPEISLGEGVAHPGLRIL
                     FDSPENYARGLFHALRVFEERGAGLIAAQWPPSEEGIGRALRDRLMRASMKE"
     gene            545..632
                     /locus_tag="PK442_09400"
     tRNA            545..632
                     /locus_tag="PK442_09400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:579..581,aa:Leu,seq:cag)
     gene            763..>2101
                     /locus_tag="PK442_09405"
     CDS             763..>2101
                     /locus_tag="PK442_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOO87909.1"
                     /translation="MAEFVPVSSKRRRLTFGRARDLVEIPDMIEVQRDSYNWFYQESR
                     EADMRTRQGLQELLQEVFPIESYDGSFALEFVRYILDDPSTEEEEARQRDLTWSRPVR
                     ATIRLVNRKTRELKEEEIFLGDFPIMTGRGTFVINGTERVVVNQLARSAGIYFTKDEM
                     IPGQEAYSAKIIPERGAWLEFALSHGEPITVNIDNRKKIPATLLLKIFGAGSNEGVLE
                     LFNARTVEKDVSEDDVRGLLSAESVLDPDGGVILPRNRLITKDHLEALWDMGRTKVRV
                     WDADPALALTLERDNAANADDAMVELFRKLRPNEPARIENAREYVHTLFFDPRRYNLG
                     RVGRYKMNRSLGIAISEEIRLLQLEDLAKIVTGLLTLKEPEKIGDDIDHLGNRRVRSV
                     GELLANQIRIGLLRMERIAKERMTTIPDLGAAMARDLINVRPIAASLREFFGSG"
BASE COUNT          426 a          610 c          647 g          418 t
ORIGIN      
        1 aatccaccgt cctcgacgcc accggcgagc agctcgttct ccttcgtccc ggagggtgcg
       61 cggtcgagga gatagaagcc cgctccggca tgaaggtgct cctctccggc ggcgcggcgc
      121 acaggtcgcc gggaacgagg tatcgtcact acgccccccg cgtgccgctg ctgctggata
      181 cggacgcgga cggaatcgct cgcgccgcgg cggaagcgga tggagcgctg cgcatcgcgt
      241 ggatcggcat gcatgcaccg gagatcagtc tgggtgaggg tgttgcgcac cccgggctgc
      301 gtatcctctt cgactccccg gagaactacg cgcgcggcct tttccatgcg ctcagggtct
      361 ttgaggagag gggagccggt cttatcgcgg cgcagtggcc tccctcggaa gaagggatag
      421 gacgcgcgct acgggacaga ttgatgcggg cttcaatgaa agagtgattt tttccgagga
      481 attctttttt cgctcttgca aaagccttct tcccttgcta caattacccg cgtggttttg
      541 aaaggccggg gtggcggaat tggtagacgc gctagattca ggttctagta ggcataacgc
      601 ctgtcggggt tcgagtcccc gcttcggcac catcgaaaat ttgagggcaa ccttttttcg
      661 aaggtcgtac tatgtctgtt gacatagtac gacctttttg ttatcttaac tctttgtcag
      721 caccattttc cccatatttt tttcagggag gtcaaaaggt tcatggccga atttgtcccc
      781 gtcagcagca agcgccggag gttgaccttt ggacgtgccc gggatttagt tgaaatcccc
      841 gacatgatcg aagtgcagcg tgattcctac aactggtttt atcaggagag cagggaagcg
      901 gacatgcgga cccggcaggg attgcaggag ctgctgcagg aagtttttcc aatagaaagc
      961 tatgacggct cgtttgccct cgagttcgtc cgctacatac tcgacgatcc ttctacggag
     1021 gaggaggagg ctcgtcagcg ggatctgacg tggtcccgtc ccgtccgtgc gacaatccgg
     1081 cttgtgaacc ggaaaaccag agagctcaag gaagaggaga tctttctcgg cgactttcct
     1141 atcatgacgg ggagaggtac gttcgtcatc aacgggacgg agcgtgtcgt cgtgaaccag
     1201 cttgcgcgtt ctgcaggaat ttacttcacg aaggacgaga tgattccggg gcaggaggcg
     1261 tactccgcca aaatcatccc cgaacgggga gcctggcttg agttcgcgct ctcccacggc
     1321 gaacccatca ccgtgaacat cgacaaccgg aagaagattc cggcgacgct gctgttgaag
     1381 atattcggcg ccggctcgaa cgagggcgtg ctcgaactct tcaacgcgcg caccgtggaa
     1441 aaggacgtct cggaagacga cgtcagaggg ctgctttccg ccgaatccgt gctcgatccg
     1501 gacggcggcg tgatcctccc gaggaaccgc ctgatcacga aagatcatct cgaagcgctc
     1561 tgggacatgg ggaggacgaa ggtccgcgtc tgggacgccg accccgccct cgcgctcacg
     1621 ctggagcggg acaacgctgc caacgccgac gacgcgatgg tggaactctt caggaagctg
     1681 cgcccgaacg agcccgcacg aatagagaac gcacgggagt acgtccatac gctcttcttc
     1741 gatccgaggc gttataacct gggccgcgtg gggcgctata aaatgaaccg gagtctcggc
     1801 atcgcaattt ccgaggaaat ccgcctgctc cagctcgaag acctggcgaa gatcgtcaca
     1861 ggtctcctga ccctgaagga accggagaag atcggcgacg acatcgacca tctcggcaac
     1921 cggcgcgtcc gctccgtcgg cgaactgctc gccaaccaga tacgcatcgg cctgctgcgc
     1981 atggagcgca tcgccaagga gcgcatgacg acgattcccg acctcggcgc ggccatggcg
     2041 cgcgatctga tcaacgtgcg ccccatagcc gccagcctgc gcgagttttt cggttcgggg
     2101 c
//
LOCUS       DAOSWW010000477         2098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43196_length_2098_cov_2.759667, whole genome shotgun sequence.
ACCESSION   DAOSWW010000477 DAOSWW010000000
VERSION     DAOSWW010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43196_length_2098_cov_2.759667"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PK442_09410"
     CDS             complement(<1..542)
                     /locus_tag="PK442_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOO87910.1"
                     /translation="MRGLFRFNEEFVYKMPVHFGGDPFYPVRTVYGDMTCLGVSFRTE
                     EDALLRFIPEDFEMLEPVVNVQYANCRDVDWMAGGEYRLIQASVPVRYEGNSEGLVGE
                     YVLVIWENKACPIIGGREEDGMPKIFADIASERHVGERWFTSASYESNTFLSLEVTRR
                     GELDEEQIAEVNKNPKINFFG"
     gene            complement(659..>2098)
                     /locus_tag="PK442_09415"
     CDS             complement(659..>2098)
                     /locus_tag="PK442_09415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOO87911.1"
                     /translation="VAVDEAHCVSQWGQDFRPSYLKIGEFVRSLPLRPVLGAFTATAT
                     PQVRDDIVQLLNLRAPHVVTTGFDRKNLYFEVQSPKSKPAALKEFLRSRREKSGIVYC
                     LTRKTVDEVCERLCDAGYSATRYHAGLTQEERRENQDRFQADDARIMVATNAFGMGID
                     KSNVAYVLHYNMPKNMESYYQEAGRAGRDGSPAECVLFYGGQDVFTNQFFIEKERENE
                     ELDEETLRKVRESERNRLRKMVAYCHTTDCLRAAILNYFGDESPAACGHCSNCLRDFE
                     QEDITIPAQKILSCVLRMKERYGVSLLVSTLRGSKSERVLNLGLGELSTYGIMKDESE
                     RSLRAKIDYLLSRGYISLTDDEFPVVKTTPLAKGVLFRGEKLAMPVAKQEPKPLAAEP
                     KGVSMLMEPTDLELFDKLRALRKEIAGAQGVPAFVVFTDATLRDMCEKLPQTPDELLQ
                     VSGVGYAKREKYGAAFLELLREHSTASVP"
BASE COUNT          380 a          668 c          610 g          440 t
ORIGIN      
        1 ccgaagaagt tgatcttcgg gttcttgttg acctcagcga tctgctcttc gtcgagttcc
       61 ccccggcgtg tgacttcgag ggaaaggaag gtgttgctct cgtagcttgc ggaggtgaac
      121 cagcgctcgc ctacgtgacg ttccgaggcg atgtccgcga agatcttcgg catgccgtcc
      181 tcctcgcgcc cgccgatgat cgggcacgcc ttgttctccc agatgaccag cacatactct
      241 ccgacgagtc cttcggaatt tccttcgtac cgtaccggga cgctcgcctg aatcagccgg
      301 tactcgcccc cggccatcca gtcgacatcg cggcagttcg catactggac gttgaccacc
      361 ggttcgagca tctcgaaatc ctcggggatg aagcgcagca gcgcgtcctc ctccgtccgg
      421 aacgaaacgc cgagacacgt catgtcgccg tagaccgtcc gcaccgggta gaacggatcg
      481 cctccgaaat gcacaggcat cttgtagacg aactcttcgt tgaacctgaa cagccctcgc
      541 atacgcaccc ctccattcga agaatatgtt cgtctattat actcttttta ctacaacaaa
      601 aagagcgcat ccccaaaaaa ggacacgctc ttcacacaaa aaatctgtta ttctgttctt
      661 acggaacgga agcagtcgag tgttcgcgaa gcagttccaa aaaggccgcg ccgtactttt
      721 cccgtttggc gtaccccacg ccgctcacct ggagcagttc gtccggcgtc tgcggcagct
      781 tctcgcacat gtcgcgaagc gtcgcgtcgg taaagaccac gaaggcggga acgccctgag
      841 cgccggcgat ctctttccgg agggcgcgca gcttgtcgaa gagttcgagg tcagtcggct
      901 ccatgagcat gctcacacct ttcggctcgg cggcaagcgg tttcggctcc tgcttcgcca
      961 cgggcatcgc gagcttctct ccccgaaaga gcactccctt tgccaacgga gtggtcttca
     1021 ccacgggaaa ttcgtcgtcc gtgagcgaaa tataccctcg cgaaagcaga tagtctatct
     1081 ttgcgcgcaa cgaacgctcg ctctcatcct tcattatgcc gtaggttgaa agttcgccga
     1141 ggccgagatt gagcacgcgc tcgctcttgc tgccgcgcag cgtgctcacg agcagcgaga
     1201 ccccgtagcg ctccttcatc ctgagcacgc aggagaggat cttctgcgcg gggatggtga
     1261 tatcctcctg ttcgaagtcg cggaggcagt tcgagcagtg accgcaggcc gcaggggatt
     1321 catctccgaa gtagttcagg atggcggccc ggaggcaatc cgtcgtgtgg cagtaggcga
     1381 ccatcttgcg cagcctgttc cgctcggact cgcgcacctt ccgcagcgtc tcctcgtcga
     1441 gttcttcgtt ctcgcgctct ttttctatga agaactggtt tgtgaacacg tcctgcccac
     1501 cgtagaagag cacgcactcc gcgggggagc cgtcacgtcc ggcgcggccc gcctcctgat
     1561 agtagctctc catgttcttc ggcatgttgt agtggagcac gtacgcgacg ttcgacttgt
     1621 cgatccccat gccgaaggcg ttcgtcgcca ccatgatccg cgcgtcgtcg gcctggaagc
     1681 ggtcctggtt ttcccggcgc tcttcctgag tcagtccggc atggtagcgc gtcgcagagt
     1741 accccgcgtc gcagagcctc tcgcagacct cgtcgacggt cttgcgggtg aggcagtaga
     1801 cgatgccgct cttctccctc cggctgcgca gaaactcctt cagcgccgcc ggcttgctct
     1861 tcggcgactg cacctcgaaa tagaggttct tgcggtcgaa gcctgtggtg acgacgtgcg
     1921 gcgcgcggag gttcagcaac tggacgatgt cgtcgcgcac ctgcggcgtc gccgtggcgg
     1981 tgaaggcccc gagcacgggg cggagcggga gggatcggac gaactcgccg atcttgaggt
     2041 agctcgggcg gaagtcctgc ccccactgcg agacgcagtg cgcctcgtcg acggcgac
//
LOCUS       DAOSWW010000478         2094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43336_length_2094_cov_1.901913, whole genome shotgun sequence.
ACCESSION   DAOSWW010000478 DAOSWW010000000
VERSION     DAOSWW010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43336_length_2094_cov_1.901913"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>256
                     /locus_tag="PK442_09420"
     CDS             <1..>256
                     /locus_tag="PK442_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOO87912.1"
                     /translation="PGKQVTLAHVICNPRPELCSLIGVEKSGAIGIMTITPGEGAIIA
                     ADIASKSGDIALDFIDRFTGCVLFTGDVAAAVHPRLDAALA"
     assembly_gap    257..266
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            422..883
                     /locus_tag="PK442_09425"
     CDS             422..883
                     /locus_tag="PK442_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HOO87913.1"
                     /translation="MRHAPGHSMKRVMIVGPTGSGKTGLLKALGIWQQDVRKTEAVSF
                     GDDAVDTPGEFFDIPHFYHALIMTSVKAGVILLVADPLKRSRHSSRFAHALRAPVIGV
                     ITKLDAASQDDVDAARKSLKNSGVSEIHAVSSLRGDGMEALGERVRKLREC"
     gene            complement(928..1485)
                     /locus_tag="PK442_09430"
     CDS             complement(928..1485)
                     /locus_tag="PK442_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO87914.1"
                     /translation="MNEVLRAIFERRSIRKYSDEPVKREDIELVLQAGLHAPTGGDAE
                     PWHFGVITDRKMIEDLDERARAAMRESGIERIVATGANPKYRIFFGAPVVIMIYGEKV
                     LRKTGTHLSALADCSAAIQNMQLAAFSLGLGTCWIGLLRYLFTTDRCMAPEGYDPLYG
                     MTLGYSAGPEAVRPRARREGTVTWF"
     gene            complement(1589..>2094)
                     /locus_tag="PK442_09435"
     CDS             complement(1589..>2094)
                     /locus_tag="PK442_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87915.1"
                     /translation="PVFRALLLGGASSTGSGSGLGESALVFFGVPEAFIGKNVENLHV
                     VKMLSTESAEVLTHVFDLDDLRDGTCAGVEIEQTAEGEKLKRVLEAKDSVTPMSRIAM
                     ALQDGGEYDLDASRNGGVTDPAFILEATLKEQVSSGGSGCSLGGEYAHLSAISLLLPA
                     LLLFLRK"
BASE COUNT          403 a          724 c          577 g          380 t
ORIGIN      
        1 cctggaaaac aggtgacgct ggcgcatgtg atctgcaacc cgcgtcccga gctctgctcg
       61 ctgatcggcg tggagaagtc gggcgccatc ggcatcatga ccatcacgcc cggcgaggga
      121 gccatcattg ccgcggacat cgcctccaag tcgggcgaca tcgcgttgga cttcatcgac
      181 cgcttcaccg ggtgcgtcct cttcaccggc gacgtcgccg ccgctgtcca tccccgcctc
      241 gacgccgccc tcgccgnnnn nnnnnnaaag ctcgctggag ggaaccgtcc ggacgctcgc
      301 caccgtgctc ggcttcaccc ccgcgccgct gacccgcacg taagcaggcg accgatgaac
      361 ggccttttcg tgagactcac tgtcgatcgc tcgcgtccca ggaagcgtag atgatgggga
      421 tatgcggcat gcccctggcc actccatgaa acgcgtcatg atcgtggggc cgaccggctc
      481 cggcaagacg ggcctgctga aggccctcgg catctggcag caggacgtcc gcaagaccga
      541 ggccgtgagc ttcggggacg atgcggtgga cacgccggga gagttcttcg acatcccgca
      601 cttctaccac gcgcttatca tgacgtcggt gaaggcgggc gtcatcctgc tcgtcgccga
      661 cccgctgaaa cgctcgcgcc actcctcccg cttcgcccac gccctccgcg cgcccgtgat
      721 cggcgtgatc accaagctgg acgcagcttc gcaggacgac gtcgacgccg cccgcaaaag
      781 cctcaagaac tccggcgtca gcgaaatcca cgccgtctcg tcgctgcgcg gcgacggcat
      841 ggaggcgctc ggcgaacggg tgcggaagct ccgcgagtgc tgaaagaaac aacgccccgc
      901 aacctgtccc tctgttccgc ttgaaactca gaaccaggtg accgtccctt cccgcctcgc
      961 tcgtggccgt accgcctcgg gacctgcgga gtaccccagc gtcatgccgt agagcgggtc
     1021 atacccctcg ggggccatgc atcggtccgt cgtgaagaga tagcgcagca gcccgatcca
     1081 gcaggtcccc agccccagcg aaaaggccgc gagctgcatg ttctgaatgg ccgcgctgca
     1141 atcggcaagc gcggaaagat gtgtccccgt cttgcggagc accttttccc cgtagatcat
     1201 gatcaccacc ggagcgccga agaagatcct gtacttcggg ttggcccctg tagcgacaat
     1261 cctctctatg cccgactccc gcatggcggc gcgggcgcgt tcgtcgagat cctcgatcat
     1321 cttccggtcg gtaatcacgc cgaagtgcca cggctcggcg tcccctccgg tcggcgcatg
     1381 gagccccgcc tgcagcacca gttcgatatc ctcccgcttc acaggttcat ccgaatactt
     1441 ccggatactg cgccgttcga atatggcgcg cagaacttcg ttcatttcac ttgcctcctt
     1501 ctgaaaaaag cccgggcgat cctcttgatg caatcacccg gacttctttt aacgcgagtg
     1561 tacgcacata tccgcgctga aaggatagtt atttcctcaa aaagagcagc aacgccggaa
     1621 ggagcaggga gatcgcggaa agatgcgcat actccccgcc tagggaacag ccgctcccac
     1681 cgctggaaac ttgctctttc aaggtcgcct ccaggataaa tgcggggtcc gtgacacctc
     1741 cattccgaga cgcgtcaaga tcgtattctc cgccatcctg aagcgccatg gcaatccggg
     1801 acatgggggt cacactatcc ttcgcctcca gcacacgctt cagcttctcc ccttcagcag
     1861 tttgttcgat ctccaccccc gcgcacgttc cgtcgcggag atcatcaaga tcgaacacgt
     1921 gcgtcaacac ctccgcgctt tccgtcgaca gcatcttgac aacgtgcaga ttttccacat
     1981 ttttccctat aaacgcctca ggtacaccga agaataccag tgctgattct ccaagccccg
     2041 atcccgagcc cgttgaagaa gcgccgccaa gtagaagcgc gcggaatacg ggca
//
LOCUS       DAOSWW010000479         2091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43420_length_2091_cov_4.051081, whole genome shotgun sequence.
ACCESSION   DAOSWW010000479 DAOSWW010000000
VERSION     DAOSWW010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43420_length_2091_cov_4.051081"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PK442_09440"
     CDS             <1..1275
                     /locus_tag="PK442_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO87916.1"
                     /translation="GFPIAFVIGSVAFFTGLAVFGPTVTFHILYSRFYDLSLNYPYLA
                     VPLFTFMGVILQHSGVTKDLYQSLYEAMGRLRGGLAVVTIIFGTILAACLGVIAASVT
                     ILTLIALAPMITRGYDKSLASGSIVAAGTLGILIPPSIMLVVYAPQAGLSVGQMFMGA
                     VFPGLLLSACYILYVVIRCRLNPALGPAIPEDQITPFDAKKMIRLLKSLVPPILLIIA
                     VLGTIFAGIAPPTEAAAVGCFAAVLLAIAYGKFSWGLMRQAMIETMRVSAFVVMIAAM
                     CYAFVGIFMNAGAGDVVAEFILSVPGGKWMSFFVIMAIVFLLGMFIEWIGIVFIVVPI
                     FSPILTALGFNPLWAGMMICINMQMAFQTPPMAMSIFVLKGTAPKELGVTIGDIIWGV
                     IPFILIIMFVLLLCAIFPGIITWLPELMIGKA"
     gene            complement(1363..>2091)
                     /locus_tag="PK442_09445"
     CDS             complement(1363..>2091)
                     /locus_tag="PK442_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87917.1"
                     /translation="HDHFQGGTWTFPMTNAPVLKSLRVAGASLEHLRWPLTTLRLRSP
                     DRTVLETLGELLLLSWREYEDRERGILARSGSGEREEQHNTVTVIGRRRGAEYEIDVV
                     LRNNRCDTQYPDGIFHAHKERHHIKKENIGLIEVMGLAILPPRLESSLNDVTAILSGE
                     KTLSDLPQYFPHTEWTVELATRFGTTLPADEAERIVRNEVGTVFSKVLRDCGVFKATE
                     DGEKGVERFLSSWAAQWGRSFASD"
BASE COUNT          368 a          623 c          581 g          519 t
ORIGIN      
        1 ggattcccca tcgcgttcgt catcgggagc gtcgcctttt tcacggggct tgcagtcttc
       61 ggtccaacgg taacgttcca catcctgtac agccgttttt acgacctgtc gctgaactac
      121 ccctacctgg cggttcccct cttcaccttc atgggggtta tactgcaaca ctcgggggtt
      181 actaaagatc tgtaccagtc gctgtacgag gcgatgggac gactccgggg cggccttgcg
      241 gttgtgacca tcatcttcgg taccatcctc gccgcctgtc tgggcgttat tgcggcttcc
      301 gtgaccatcc tgacgctcat cgccctcgcg ccgatgatca ccagagggta tgataaatca
      361 ctcgcatcgg gatccatcgt agcggcggga acgctcggca tcctcatccc gccgagcatc
      421 atgcttgtcg tctatgcgcc gcaagccgga ctctcggtcg ggcagatgtt catgggggcc
      481 gtattcccgg ggctgctgct ctctgcgtgc tacatcctct acgttgtgat ccggtgcagg
      541 ctcaacccgg cgctcggtcc cgcgatcccg gaagatcaga taacgccgtt cgacgccaaa
      601 aaaatgatcc gccttctcaa atcccttgtg ccgcccatac tgcttatcat tgcggtgctc
      661 gggactatct tcgccggaat tgcaccaccg actgaagccg ccgcagtagg gtgtttcgcg
      721 gccgttctcc tcgccatagc ctacgggaaa ttcagctggg ggctgatgcg tcaggccatg
      781 atcgagacga tgcgcgtaag cgctttcgtc gtgatgatcg cggcgatgtg ctatgccttc
      841 gtcggcatct ttatgaacgc aggggctggc gacgtcgtgg cggagttcat cctctcggta
      901 ccgggaggga agtggatgtc cttcttcgtc atcatggcga tcgtcttcct tctcggcatg
      961 ttcatcgagt ggatcggcat cgtcttcatc gtggtgccta ttttctcgcc cattctgaca
     1021 gctttgggat tcaacccgct ttgggccggc atgatgatct gcatcaacat gcagatggcc
     1081 ttccagacgc cgccgatggc gatgtcgatc ttcgtcctca aggggacggc gccgaaagaa
     1141 ctcggagtaa cgatcggcga catcatctgg ggcgtcattc cgttcatcct catcatcatg
     1201 ttcgtccttc tgctctgcgc catcttcccg gggatcatca cctggctccc cgaactgatg
     1261 atcggcaagg cgtagagacc tcagtcatac cgaaacacaa acggcggccc gcttttttcg
     1321 gggtcgccgt tctttttgga gacacactcc ttgggggcct cttcagtctg atgcaaacga
     1381 acgcccccac tgcgccgccc aggaggagag aaatcgttcg actccttttt ctccgtcctc
     1441 agttgccttg aacactccgc aatccctgag gactttggaa aaaactgtgc cgacctcgtt
     1501 ccggacgata cgctctgctt cgtctgcagg aagagtggta ccgaaacggg ttgccagttc
     1561 aactgtccat tcggtgtggg gaaagtactg cggaagatcc gaaagagttt tttctcctga
     1621 gagaatcgcg gtcacgtcgt tcaggcttga ttccagacgg ggaggaagaa tcgcgagtcc
     1681 cataacttcg atgagtccga tattctcttt tttgatatga tggcgttcct tgtgcgcgtg
     1741 gaatatgccg tcggggtact gagtgtcgca gcgattattc cgcagcacga cgtctatttc
     1801 gtactctgcg ccccgccgtc tgccgatgac cgtgacggtg ttgtgctgtt cctctctttc
     1861 tccgcttccg cttcgtgcga gtattccccg ttccctgtcc tcgtactcgc gccaggagag
     1921 gagcagcagc tcgccgagcg tttccaggac ggttctgtcg gggcttctca ggcgaagggt
     1981 tgtcaagggc cagcggagat gttccaggga tgcgccggcg acacgcagac tcttcagaac
     2041 aggcgcgttc gtcatcggaa atgtccacgt ccctccctga aagtgatcgt g
//
LOCUS       DAOSWW010000480         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43464_length_2090_cov_3.521867, whole genome shotgun sequence.
ACCESSION   DAOSWW010000480 DAOSWW010000000
VERSION     DAOSWW010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43464_length_2090_cov_3.521867"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK442_09450"
     CDS             complement(<1..515)
                     /locus_tag="PK442_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87918.1"
                     /translation="MMTNDPGRRKRLLLVGAGHAHLFSFARIERFLRGGIDVTVISSA
                     PLRFFGTGPEVLGDSDGMKELTVDVRALVERGGGRFLEGRGASVHPQEHCVRTESGEE
                     LFFDALSLNIGSEIPSALLGGSPGRVFAVRSISSLLELRRRILALPPGAKPHLAVARG
                     GPSGCEAAANV"
     gene            complement(810..1052)
                     /locus_tag="PK442_09455"
     CDS             complement(810..1052)
                     /locus_tag="PK442_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87919.1"
                     /translation="MDVGTLRWFFFWCAIFNFFILGGFFLGFIFAGDMIYSFHRKWFT
                     LDRDAFNTVMYFFLGIYKILILVFNFVPWLALLIAG"
     gene            complement(1146..1700)
                     /locus_tag="PK442_09460"
     CDS             complement(1146..1700)
                     /locus_tag="PK442_09460"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOO87920.1"
                     /translation="MPVVSSFPPVARTDAVICILGSMPGRASLEADQYYAHPRNAFWR
                     LLESVLGIPCSLPYPERLERLKERRTALWDVLGTCARDGSLDSRIEPSSIIPNDFATF
                     FAMHPLIGTVFFNGGKAEAVYRREVLPTLAEPARSIRLLRLPSTSPAHAGMTFEQKKD
                     AWGRLGRVLEENASQGYHERGNDC"
     gene            complement(1737..>2090)
                     /locus_tag="PK442_09465"
     CDS             complement(1737..>2090)
                     /locus_tag="PK442_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87921.1"
                     /translation="IKKLDLLAEECIKAMETDDTLEFKGQVFEADGVDPLESLDDIHS
                     EFMARLATKMLWPRADVLAVCDALSIAPEPSFELLNAWGRIAGGLPLIDDGDPVFVDI
                     PLLQEMLLRENADAQ"
BASE COUNT          527 a          604 c          563 g          396 t
ORIGIN      
        1 tacacgtttg cggccgcttc gcatcccgag ggacctcctc tcgcgacagc aaggtgcggc
       61 ttcgcaccgg gaggcaaggc aagtatccgc cgtcgcagct cgaggagcga ggatatggac
      121 ctcacggcaa agacgcgccc cggcgagccg ccgagaagag cggacggaat ttcgctgccg
      181 atatttaaag agagcgcgtc aaaaaaaagt tcctctccgc tctccgttcg cacacagtgt
      241 tcctgcggat gtacggaagc gcccctcccc tccaggaagc gccctccccc gcgctcgaca
      301 agggcgcgca catctacggt caattccttc atcccatcgg aatcgccaag tacttcaggc
      361 cccgtcccaa aaaaccggag gggcgcggaa gagatcacag tcacatcaat cccgccgcgg
      421 agaaaccgtt ctatgcgcgc gaagctgaaa aggtgcgcgt gcccggcgcc tacaaggagc
      481 aggcgttttc ttctccccgg atcattcgtc atcatgcgca acctcctttc ctgaacagac
      541 tggtttcaaa ccatgaaaag cattataaac catgagagca ggccgtatct tcagacttct
      601 tgagttctgc cgatgactgt ttactgggaa cggaaccttc ttggcacgca ggagacaccc
      661 ggcacaaagg gtggggtgca gacggatcaa gggcgcagtc gacgtgaaat cgcgctttga
      721 gggaataaag gacgaacgga aaggggtttt caatagaagg gcgcgtccca gagcgaggga
      781 aaaacagcta tggaacgcgc ccctcgtaat tagccggcga tcagaagcgc gagccaggga
      841 acgaaattga agaccagtat caggattttg tagattccca aaaagaagta catcaccgtg
      901 ttaaaagcat ccctgtccag cgtgaaccac ttgcggtgaa aagaatagat catgtcgccg
      961 gcaaaaatga atccgagaaa gaagcctcca agaataaaga aattaaaaat ggcgcaccag
     1021 aaaaagaacc aacgcagtgt accgacatcc attacaaacc ctccctttgt tatgatccat
     1081 cgaatcagga gaccacctgc ggtcacggag gtactcccgg gaagattgat atgctcactc
     1141 tctcctcagc aatcgttccc ccgctcatgg tacccttgac tggcgttttc ttcaaggact
     1201 ctcccgaggc ggccccacgc atccttcttc tgctcgaagg tcattcctgc atgcgcggga
     1261 cttgtagagg ggaggcggag aagtcgaatg gatcgggcag gttcggcaag agtcgggagt
     1321 acttctctgc gatagaccgc ctcagccttt ccgccgttga aaaaaacagt tcctataaga
     1381 ggatgcatgg cgaaaaaagt cgcaaaatcg ttgggaatga tactggacgg ttcgatacgt
     1441 gaatcaaggc tcccgtcgcg agcgcacgtc ccaaggacgt cccagagcgc tgtacgtcgt
     1501 tctttcagcc gttcaagcct ctccggatat ggcaacgagc agggaatacc cagtacggac
     1561 tcaagaagac gccagaaggc atttcgaggg tgcgcatagt actggtcggc ttcaagcgac
     1621 gccctgccgg gcatgctgcc gagaatacag atcacggcgt ccgtccgggc gaccggggga
     1681 aaacttgaaa ctaccggcac aaaataacct tcctctcctg aggcgctccc ggcacgctac
     1741 tgggcatctg cattctcccg aagcagcatc tcctgcaaca aagggatatc cacaaaaacc
     1801 ggatccccgt cgtcaatcag gggcagtccc ccggctatcc gcccccacgc gttcagcagc
     1861 tcgaaggagg gttccggcgc aatggaaagg gcgtcgcaga ccgccagaac atccgctctc
     1921 ggccagagca ttttcgtcgc caggcgggcc ataaattcgc tgtggatatc gtccagagac
     1981 tcgagaggat cgacgccgtc ggcctcgaac acctgtcctt taaattcaag agtgtcatcg
     2041 gtctccatcg cctttatgca ctcttcggca aggaggtcga gtttctttat
//
LOCUS       DAOSWW010000481         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43469_length_2090_cov_3.029484, whole genome shotgun sequence.
ACCESSION   DAOSWW010000481 DAOSWW010000000
VERSION     DAOSWW010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43469_length_2090_cov_3.029484"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PK442_09470"
     CDS             complement(<1..199)
                     /locus_tag="PK442_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87922.1"
                     /translation="MLRRNRISFMEERLRRLEEQNAYLWQEKAFGIEALDAAASLGHF
                     DTSMNKLEDPLPILRETVTRVQ"
     gene            complement(201..1460)
                     /locus_tag="PK442_09475"
     CDS             complement(201..1460)
                     /locus_tag="PK442_09475"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOO87923.1"
                     /translation="MGLLHVDQLQAGMVLEKNLLAFNGRFLLPRGIVLTEKHIQTMKV
                     WGVVEASVEGDLPAEPLKDAAASMPPEVVQEAGRSLETLFPSTPSTSPMKDFYSVAME
                     RTTAALMRDRSQRGAQAQQPRSPARRLSSNIPNLSVTASDLVSRELQLASLPDVYYRI
                     MEVINSTRSSASHIAEAVGKDTSLLSRLLRLVNSAFYGFPSKIETVTRAVAIVGTREL
                     STLALGVAAVQYFNDIPSEYVDMKSFWRHSVACGVYARLMASEKIGVSEERLFVSGLL
                     HDLGKLVLFRQVPLSARNAIELALAKKIPLCEAEREILGFDHAYLGALLLREWKIPSP
                     LENAVRFHHSPLSSSSPLDPALLASADALAMATGFSGSGSVVMPQISPEIWAETGLSA
                     AVFQPVILQGSRQIEDITNAFLGNGGA"
     gene            complement(1557..>2090)
                     /locus_tag="PK442_09480"
     CDS             complement(1557..>2090)
                     /locus_tag="PK442_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87924.1"
                     /translation="SLKRLDDRKEPLLRRTALLASFRRKLSFPDLEGASSVRAILEGW
                     RWPKQVCSGCAALVCRRAAPAANMSRAEMADLLLAHGEAFWESVFALFGALCRVHVPR
                     RRMALFRERFGRLAVRLRTEEALIPSGDELMERYALSSGPEVGRLLKALRTEHLLKGF
                     ASREEAMERVASLMNIR"
BASE COUNT          460 a          633 c          628 g          369 t
ORIGIN      
        1 cctgcactcg cgtgacagtc tcccggagaa tcggcagcgg gtcttcgagc ttgttcatgc
       61 tggtgtcgaa gtgccccaac gaagcagcgg catcaagcgc ctcgatgccg aatgcttttt
      121 cctgccagag gtatgcgttc tgttcctcca ggcggcggag gcgttcctcc ataaaagaga
      181 tgcgattgcg acggagcatg ctatgcgcct ccgtttccga gaaacgcgtt cgtaatgtcc
      241 tctatctggc ggcttccctg aaggatcacc ggctggaaga cggccgcgga gagccccgtc
      301 tccgcccata tctccggcga gatctggggc atcacgaccg agccgctccc gctgaatcct
      361 gtcgccatgg cgagcgcatc cgccgacgcc agaagcgcag gatcaagagg agaggaagag
      421 gaaagcgggg agtgatgaaa ccgtacggcg ttttcgagag gcgagggaat tttccactcc
      481 cgcagcagca acgccccgag ataggcgtgg tcgaagccga ggatctcccg ctccgcttca
      541 cagaggggaa ttttcttggc aagagccagt tcgatggcgt tccgcgccga aagaggcacc
      601 tgacggaaga gcacgagctt tcccaggtcg tgcagcagtc cggagacgaa gagacgttcc
      661 tcggaaacgc cgatcttctc cgaggccatc agacgggcgt agacgccgca ggccacggag
      721 tgcctccaga agctcttcat gtccacatat tccgagggga tgtcgttgaa gtactggacg
      781 gccgcaaccc cgagcgccaa cgtcgaaagc tcgcgggttc cgacgatcgc aacggcccgc
      841 gtcacggtct ctatcttgga ggggaagccg tagaacgcgc tgttgacaag acgaagaagc
      901 cgggaaagaa gactggtatc cttcccgacg gcctcggcta tatgggaggc ggagctgcgg
      961 gtcgaattga tcacctccat gatccgatag tacacatcgg ggagagaggc aagctgcagc
     1021 tccctggaga cgagatcgga ggctgtcaca gacagattgg gaatattcga ggacaaccgg
     1081 cgcgcggggc tgcggggctg ttgcgcctgc gccccgcgct gcgaccggtc gcgcatcagg
     1141 gctgcggttg tccgttccat ggcgacagag tagaaatctt tcatcggcga agtcgacgga
     1201 gtggagggga agagcgtctc cagcgagcgc ccggcctcct gcacaacctc gggaggcatt
     1261 gaggctgcgg catccttcag aggctccgca gggagatctc cctcaacgga cgcctctaca
     1321 accccccaga ccttcatcgt ctgaatatgc ttctccgtaa gcacaatacc ccttgggaga
     1381 agaaagcggc cgttgaaagc cagcagattc ttctccagta ccatgcccgc ttgcaactga
     1441 tcgacatgaa gcagacccat gaaaatcgac tccctctata ccctctgcag atacaaatat
     1501 gtttcttttg attgtaacac gaagagagcg gaaaacagag aggggagttc ctctcttcat
     1561 cgtatattca tcaacgaagc gactctctcc atcgcctcct ccctcgaagc gaatcccttg
     1621 agaagatgtt cggtccgaag agccttcaag agtctcccca cctccggacc tgaagagagc
     1681 gcataccgct ccatcagctc atctccggag ggaatgagcg cttcttccgt ccggaggcga
     1741 acggcgaggc ggccgaaacg ctcccggaag agagccatgc gcctgcgcgg gacatgcact
     1801 cggcagagag cgccgaaaag agcaaaaacc gactcccaga aggcctcgcc gtgagcaaga
     1861 agcagatcgg ccatctccgc ccgcgacatg ttcgccgcag gggcggccct cctgcacaca
     1921 agcgccgcgc atcccgagca cacctgtttc ggccagcgcc atccttccag tattgcgcgg
     1981 accgaggagg cgccttcgag gtcggggaag gagagcttcc tccggaacga ggcgagaagc
     2041 gccgtccgtc tgagaagggg ttctttccgg tcgtcaagcc tcttcagcga
//
LOCUS       DAOSWW010000482         2088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43525_length_2088_cov_3.892769, whole genome shotgun sequence.
ACCESSION   DAOSWW010000482 DAOSWW010000000
VERSION     DAOSWW010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43525_length_2088_cov_3.892769"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PK442_09485"
     CDS             <1..540
                     /locus_tag="PK442_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO87925.1"
                     /translation="MSESIRTTASEGPVLFSVLRKGETLSLTVTIPPDAQGIPLLGIR
                     PAMRRYSVLEAFASAFTYTVDMTFEMVRGIIRWITGVEKVDVTGPVGIASMAGDAARR
                     GMWPFVTFLALINLNLGLLNLFPFPALDGGRLIFTAGEMLLRRRLPARIENFIHMSGF
                     VLLLLLIVYVTWQDILRFF"
     gene            556..1641
                     /gene="ispG"
                     /locus_tag="PK442_09490"
     CDS             556..1641
                     /gene="ispG"
                     /locus_tag="PK442_09490"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOO87926.1"
                     /translation="MSSRQLALRDLTIGGEAPVRVESMLKIPLSLPEDCLAQCFSLRK
                     VGCELVRVAFPSADLRGNLEWLNDRSPIPLMADIHFDGSLALAALESGIPSIRINPGN
                     MGPSHLRSIVASANEKKAVIRIGANGGSLSSSQLDAAGGVRSDALAAAVEEQLRILLA
                     EGFEDIILSAKSTSVMETVRANTLLASKYPDFPMHIGITESGYGRDGLVKSAAGLSLL
                     LAQGIGNTLRISLTEKPEEEVLAGYALLRALDIRSHGVTIVSCPTCGRRRIDVRSILE
                     KLEPSFADLPDALTVAVMGCEVNGPREAMDADFGVAGSPGGAVIFKKGKVLREVSLDE
                     LPGVFADFACSSGENHPSNSCEHLKGE"
     gene            1648..>2088
                     /locus_tag="PK442_09495"
     CDS             1648..>2088
                     /locus_tag="PK442_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOO87927.1"
                     /translation="MALNLKGRSFLTLMDFTPEEIYYLLDLSADLKAKKRSGIRGTAL
                     QGKNVALIFEKSSTRTRCAFTVACIDEGAHPEFLSKNDIHLGAKEDVKDTARVLGRMF
                     DGIQYRGFKQSLVEELARYAGVPVWNGLTDVDHPTQILADFLTIQ"
BASE COUNT          446 a          551 c          564 g          527 t
ORIGIN      
        1 atgtctgaga gtattcgtac aacagcctcg gaaggacctg ttcttttttc ggtgctgcgg
       61 aagggagaaa ccctctcttt gacagtaacc attccgccgg atgctcaggg gattcctctt
      121 cttggaatac gaccggcgat gcgccggtat tccgttcttg aggcttttgc atccgcattc
      181 acctacactg tcgatatgac ttttgaaatg gtgcggggaa ttatccgctg gataaccggg
      241 gttgaaaaag tcgacgtcac cgggcccgta ggtattgcct ccatggcagg agacgctgcg
      301 cgcagaggga tgtggccttt tgtcacattt cttgccttga tcaatctgaa cctggggctg
      361 ctcaatctgt tccccttccc agcccttgac ggaggccgtc tcatctttac agcgggtgaa
      421 atgttgctca gaagacgcct tccggcacga atagaaaatt ttattcatat gtcgggtttt
      481 gttttgttac tcctgcttat cgtctatgta acatggcagg atatccttcg ttttttctag
      541 aggaagtgaa agaatatgag ctctcgtcag cttgctctgc gtgatctcac aataggcggc
      601 gaggcgccgg ttcgcgttga gagcatgctc aagattcctc tctctcttcc ggaggactgt
      661 ctcgcgcagt gtttctcact gcgtaaggtc ggatgcgagc tggtgcgggt tgcatttcca
      721 tcggccgatc ttcgtggcaa cttggagtgg ctgaatgatc ggtcgccgat tccccttatg
      781 gccgatatcc atttcgatgg atcgcttgct cttgcggctc tcgaatcagg aattccttcg
      841 atccggatca atccgggaaa catggggccc tctcaccttc ggagtatagt ggcttccgcc
      901 aatgagaaaa aagccgtgat ccgtatcggc gcgaacggcg gctctctctc ttcttctcaa
      961 ctcgatgctg cgggcggcgt tcgatccgac gctcttgccg ccgccgtaga agaacagctc
     1021 cggattctgc ttgccgaagg gttcgaggat attatcctct cggcgaaatc gacctccgtc
     1081 atggagacgg tgcgtgcgaa cacactgctc gcttcgaagt atcctgattt tccgatgcac
     1141 atcggcatca ccgaatcggg ctatggaaga gacggactcg tcaaaagcgc tgccgggctc
     1201 tcgctgcttc tcgcacaggg aatcggcaat acgctccgga tcagtcttac cgagaaaccg
     1261 gaggaagagg ttctggccgg atacgccctt ttgagagcgc ttgacattcg atctcacgga
     1321 gtcactatcg tttcgtgccc tacatgcgga cgaagacgta ttgacgtgcg ttccatcctg
     1381 gaaaaattag aaccctcttt tgccgatctc cccgatgcgc tgaccgtcgc ggtaatgggc
     1441 tgcgaggtga acggcccgag agaggcgatg gacgccgact tcggcgttgc cggctcgcca
     1501 ggcggcgccg tcatcttcaa aaagggaaaa gtcctgcggg aagtctcctt ggatgagctt
     1561 cccggtgttt tcgccgactt tgcttgtagc tcgggtgaaa accacccgag taattcatgc
     1621 gaacatctga aaggagaatg atgtactatg gctctcaatc tcaaaggacg cagttttctc
     1681 actctgatgg attttactcc cgaagaaatt tactatcttt tggacctctc cgcagatctg
     1741 aaggcaaaaa aacgttccgg aatccgggga acggcgcttc aggggaaaaa cgttgcactt
     1801 attttcgaaa agtcgtccac cagaacccgc tgtgctttta ctgtcgcatg tatcgacgaa
     1861 ggagcccatc ctgaatttct gagcaagaac gatatccatc tgggcgcgaa agaggatgtc
     1921 aaggacaccg cccgggtgct tggaagaatg ttcgacggta ttcagtaccg cgggttcaaa
     1981 cagtcgctgg ttgaagagct tgcccggtat gccggcgttc ccgtatggaa cggactcacc
     2041 gatgtggacc acccgacaca gattctcgca gactttctaa caatacag
//
LOCUS       DAOSWW010000483         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43720_length_2082_cov_2.741983, whole genome shotgun sequence.
ACCESSION   DAOSWW010000483 DAOSWW010000000
VERSION     DAOSWW010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43720_length_2082_cov_2.741983"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..912)
                     /locus_tag="PK442_09500"
     CDS             complement(109..912)
                     /locus_tag="PK442_09500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO87928.1"
                     /translation="MERDEAMSASAVAENFSATLRLWGALPTSRAEARGPCLFVSTGT
                     AIPDQNYAYCPVEEDAEENVRSSARFFREERMPFTWWFPPGTRGERFRRALEEQRMPE
                     RCAPPAMLMALDAPAERAHHSAERTVCKAVSSAEAEEWARSSLEGFDSGPEHQGPFSA
                     FCTSLLVPPFDEAFRLLTLRVGGRAAATALLSLTGEVAGIYYFSTLPAFRRRGLGRLL
                     LSEALEEARRSGCRTAVLQASPMGFALYKKAGFAVCGRFAVHSADPDAC"
     gene            complement(953..1225)
                     /locus_tag="PK442_09505"
     CDS             complement(953..1225)
                     /locus_tag="PK442_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87929.1"
                     /translation="MFERIISILLGSAILYLVRFKLLRKPLSPIRSILVATVIMVAGI
                     QFHYAYLPYETFQGGVTYTSLGFIAFLMFQYGYRKTAPSKKESAEK"
     gene            complement(1250..>2082)
                     /locus_tag="PK442_09510"
     CDS             complement(1250..>2082)
                     /locus_tag="PK442_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87930.1"
                     /translation="TEVQMRTRKYREAEEKERQQMAVQNHAFIEAEKRIASALKGNEE
                     EEGLDENEIAKVTKLLESAKTLARNIVYVEQELVKWDRKVKKKAADLTAEAPAFRRRE
                     LRNMIEAKKEYISLTAKSARLDDSQLCQSDKLPFSIDKTAAILEEMIALDPEMLVVAR
                     VRMYGIPRVILVPGQGYGTYDWNDHTLLIPAFPTYSQERAAAYALGTFRWDSDEDRVI
                     KNSYELIKENRDKSILDLNTSFYKDYFLWLTKEKKGYRILPRSTHKVFVQMFAPQKQE
                     "
BASE COUNT          373 a          623 c          636 g          450 t
ORIGIN      
        1 aaccgtgctc gcggcgggag acaagatagt ggcgcttgtc gacgggcgga gggcgcggca
       61 tgtccgggaa gcgctgcacg acctcctgga ggttcgtcga acaacgactt aacaggcgtc
      121 gggatcggcg gagtggaccg caaaacggcc gcacacggcg aaaccagctt ttttatacag
      181 cgcaaatccc atgggggagg cctggagcac ggctgtacga cagccggatc gccgcgcctc
      241 ctccagcgct tccgacagca ggagcctccc cagccctcgc ctgcggaagg cggggagagt
      301 ggagaagtag tagattcctg ccacctctcc ggtcagagag agaagggcgg ttgccgcagc
      361 gcgccccccc acgcgaagcg tcagcaatct gaacgcttcg tcgaaaggcg gtacgagaag
      421 cgacgtgcag aatgcggaga aaggtccttg atgctccggc cccgagtcga atccttcaag
      481 ggaggagcgg gcccattcct cggcctcagc ggatgagaca gccttgcaaa cggtgcgctc
      541 cgcagaatgg tgtgcgcgtt ccgccggtgc gtcgagcgcc atgagcatcg ccggcggcgc
      601 gcaccgctcg ggcattcgct gctcctcaag cgctctccgg aagcgttctc cccgtgttcc
      661 gggggggaac caccacgtga agggcatcct ctcctcgcga aaaaaacgcg ctgaactccg
      721 tacgttctcc tcggcgtcct cttccacggg gcagtacgcg tagttctggt ccggtatggc
      781 cgttccggtc gacacgaaga gacaggggcc gcgcgcctcg gctctcgacg tcggcagcgc
      841 cccccagagt cgcagcgtcg cgctgaagtt ttccgcaaca gcagatgcac tcatcgcttc
      901 gtctctctcc attgccgctc cgccttttta tgaagtctgc agcgtggacg ggctactttt
      961 ctgcgctctc tttcttggag ggagcggttt tgcggtagcc gtactggaac atcaggaacg
     1021 caatgaagcc aagcgacgtg taggtgacgc ccccttgaaa cgtctcgtag gggagatagg
     1081 cgtagtgaaa ttgtattccg gcgaccatga tgaccgtcgc gacgagaatg gatcgaatcg
     1141 gcgagagcgg tttccgcagc agcttgaagc gcacaagata gaggatggcg ctgccaagaa
     1201 gtatactaat gattcgttca aacattggac tctcctttta ctctgttttt cattcctgtt
     1261 tctgaggcgc gaacatctgg acgaagacct tgtgcgtgct gcgcggcagg atgcggtagc
     1321 ccttcttctc tttcgtcagc cagaggaaat agtccttgta gaacgacgtg ttcaggtcga
     1381 ggatggactt gtcgcggttc tccttgatga gctcgtagct gttcttgatg acgcggtcct
     1441 cgtcgctgtc ccagcggaac gttcccaggg cgtaggctgc ggcgcgctcc tggctgtagg
     1501 tcggaaacgc cgggatcagc agggtgtggt cgttccagtc gtacgtgccg tacccctgtc
     1561 cggggacgag gatcacgcgc ggtatgccgt acatgcgaac tcgtgccacg acgagcatct
     1621 ccgggtcgag cgcgatcatc tcctcaagaa tggcggcggt tttgtcgatg gaaaaaggaa
     1681 gcttatcgct ctggcagagc tgtgagtcat ccagccgggc ggacttcgcc gtaagcgaga
     1741 tgtactcttt cttcgcctcg atcatgttcc ggagttccct gcgccggaac gccggggctt
     1801 ccgccgtgag atcggccgcc ttctttttga ctttccggtc ccatttaacg agttcctgtt
     1861 ccacgtagac aatgttccgg gcgagcgtct tcgcgctctc aagcagcttc gtgaccttgg
     1921 cgatctcgtt ttcgtcaagc ccctcttctt cctcgtttcc cttcagggcg cttgcgatgc
     1981 gcttctcggc ttcgatgaaa gcgtgattct gcacggccat ctgctggcgc tccttctcct
     2041 cggcctccct gtacttcctg gtgcgcatct gtacctcggt ac
//
LOCUS       DAOSWW010000484         2081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43742_length_2081_cov_3.917078, whole genome shotgun sequence.
ACCESSION   DAOSWW010000484 DAOSWW010000000
VERSION     DAOSWW010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43742_length_2081_cov_3.917078"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="PK442_09515"
     CDS             <1..103
                     /locus_tag="PK442_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOO87931.1"
                     /translation="KELSMGMSGDFEWAVDEGSTMVRIGSAIFGKRT"
     gene            128..508
                     /locus_tag="PK442_09520"
     CDS             128..508
                     /locus_tag="PK442_09520"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOO87932.1"
                     /translation="MLERMMAFLGLVDPAAEEEEGAGETAELETREARVLKPVPESAV
                     ILIARGLEGVQRKEELALALRKGKLLLVDLRGLEREEGQSLLDFLCGVAFANRGTVLR
                     AASGIFLAAPRKSMIEEWEEEDGA"
     gene            501..1004
                     /locus_tag="PK442_09525"
     CDS             501..1004
                     /locus_tag="PK442_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOO87933.1"
                     /translation="MPDLLTARDVESKRFSKAMRGYSATEVDQFLDQVAEDIQKYCVQ
                     YADLERYAARLEDKIREFESLKETLSGTLLMAQKTADAKEEAANRQSEAIILEAKSKA
                     EQIIQEAEASKERERKEINHIRQLKQEFKADFCALLSRFSSLIERDEEDPDENDGAEP
                     TEDDVLK"
     gene            1001..>2081
                     /locus_tag="PK442_09530"
     CDS             1001..>2081
                     /locus_tag="PK442_09530"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO87934.1"
                     /translation="MTIRRADLSDPVRFVKGVGPSRERQLANLGVFTAEDLLFLFPRR
                     YEDRRTLTPLSSLRPGTATTVIARVVAVEKRTTAKKQLSLTTVLISDGQGMAKAVWFN
                     RVGLENTFRSGLRVALFGKVELRNGVPQLVAPEFEVLEEDDGEEELLSIVPLYPATAG
                     LHQKTLRRIVRTALEEYLPLLEEFLPVSLRERRRLCELTEAMREIHYPTSRENWKTAR
                     RRLVFDEFFLLQAGLALRRNRAEKPLERAPSLVRGHFVEEYMNRFLPFVLTEGQRSAV
                     EDILSDMERTAPMNRLLQGDVGSGKTVVALIALLAALDGECQGALLAPTEILARQHFL
                     RLSPVFTALGIPCALLTGSLGVKEKK"
BASE COUNT          464 a          525 c          642 g          450 t
ORIGIN      
        1 gaaagagctt tccatgggga tgagcggtga ttttgaatgg gctgttgatg agggaagtac
       61 gatggtccgt atcggctccg ctatcttcgg aaaacgtacc tgatacgccg cagcgggagg
      121 tgatgccttg ttggaacgaa tgatggcctt tctggggctc gttgacccgg ctgccgaaga
      181 agaggaaggc gcgggggaga cggcggaact cgaaacaaga gaggcccgcg tactgaagcc
      241 ggttcctgaa agcgcggtga ttcttatcgc ccgcggtctt gagggagtac aaagaaaaga
      301 agagcttgcc ctggctctcc gtaaggggaa acttctgctc gtcgatcttc ggggtcttga
      361 acgggaagag ggacagagtc tgctcgattt cctctgcgga gtggcttttg caaacagagg
      421 tactgttctg cgggcagcaa gcgggatttt tctcgcggcg ccgcggaaga gcatgataga
      481 agaatgggag gaagaagacg gtgcctgatc tgctgacagc tcgcgatgtg gaaagcaaac
      541 gcttttcaaa ggcgatgaga gggtattccg caacggaagt tgaccaattc ctcgaccagg
      601 ttgccgagga tatccagaag tactgcgtcc agtatgccga tcttgaacgc tatgccgccc
      661 gcctggagga caagatccgg gagtttgaaa gcctgaaaga aacgctctcc ggaactctgc
      721 tcatggcgca gaaaactgcg gatgccaagg aagaagctgc aaaccgccag tccgaggcta
      781 tcatactgga ggcgaagtcg aaggcggaac agatcattca ggaggcggaa gcctcgaagg
      841 agcgggagcg caaggagatc aatcatatca ggcagctcaa acaggagttt aaagccgact
      901 tttgcgcact cctctcccgt ttttcttccc ttattgaacg cgatgaggaa gatcccgacg
      961 agaacgacgg tgctgaaccg acggaggacg atgtgctcaa gtgactattc gccgagcgga
     1021 cctgtccgac cccgttcgtt tcgtgaaggg agtcggccct tccagagaac gtcagttggc
     1081 caacctcggt gttttcacgg cagaggatct cctgtttctt tttccccgcc ggtacgaaga
     1141 caggagaacg ctgacgccgc tttcgtctct caggcccgga acagccacga cggtgatcgc
     1201 cagagttgtc gctgttgaaa aacggacaac ggcaaaaaag caactctctc tgaccactgt
     1261 gctgatttcc gatggtcagg ggatggcgaa ggctgtctgg ttcaacagag ttggacttga
     1321 gaacaccttt cgttcaggac tcagggttgc tctgttcggt aaggtggagc tgcgcaacgg
     1381 cgttcctcag cttgtagcgc ctgaatttga agtgcttgaa gaggatgacg gagaagagga
     1441 gctgctctcg attgttcctc tgtatccggc gactgcgggg ctgcatcaga agacgctgcg
     1501 ccgcattgtg cggacagctc tggaggagta tcttcccctg ctggaagagt ttcttcccgt
     1561 atctttgcgg gagcgccgcc gcttatgcga attgactgag gcgatgaggg aaatacacta
     1621 cccgacgtcg cgggagaact ggaagactgc caggagacgc cttgtcttcg acgaattctt
     1681 tctcctgcag gcggggcttg cgctgcgaag gaacagggcc gaaaaacccc ttgaacgagc
     1741 cccttcgctt gtccgtgggc attttgtcga agagtatatg aatcgcttcc ttccgtttgt
     1801 gcttacagaa ggacagagga gcgccgtgga ggacatcctc tcggatatgg agcgcacggc
     1861 gcctatgaat cgtcttcttc agggagacgt gggatcggga aagaccgtcg tcgcgcttat
     1921 cgcattgctc gccgcgctcg acggggagtg tcagggggct cttctggccc cgaccgagat
     1981 tcttgcccgc cagcacttcc tccgcctttc acctgttttt actgctcttg gaattccgtg
     2041 cgccttgctg accggctctc tcggcgtcaa agaaaaaaaa g
//
LOCUS       DAOSWW010000485         2076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43920_length_2076_cov_4.163780, whole genome shotgun sequence.
ACCESSION   DAOSWW010000485 DAOSWW010000000
VERSION     DAOSWW010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43920_length_2076_cov_4.163780"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /gene="feoB"
                     /locus_tag="PK442_09535"
     CDS             <1..1784
                     /gene="feoB"
                     /locus_tag="PK442_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOO87935.1"
                     /translation="AVVVADASNLERSLYLAVQMLEIGTPLVLALNMMDMAAERGIMI
                     DVERLSALLGIPVVPTVARKKEGIEELKERITRSLRENSAPSLSFALPYGERLNPLFE
                     ALETFLGERQELIPGLSARSAAVKFVEGDVAVLAAAESSGVRNSMDELLAEKGRKVEE
                     ELGYDLQTAVIERRWGFVSGVTAESVQRDLSLKARLTLSDRIDRVVTSRSLGLPLFFL
                     VTWAIFRMTYALGDPLVELLEGVFESFGEWTGEFFAGAGFSEIVSSFVQDGLIGGVGS
                     VVVFFPHIFILFAFIALLEDSGYMARGAFVMDRVMHLMGLHGKSFIPMLMGFGCNVPS
                     MMATRILEQPRDRMITLLVLPFMSCSARLPVFVLFSGIFFGAHAGTAVFSLYVLGIIV
                     AVLAAKVLGSTLFKGESSQLVMELPPYHVPSLSMVLRHAWERGFLFLRKAGTFILLSV
                     IAVWVLASFPWGVEYGSSESIVGRIGQILAPVFAPAGFGFWQAAVALFFGFLAKEVVV
                     GTLGALLGAGEAGLAAALPGLFTPLSAYAFLVMTLLYVPCVAAVAAFRRETGSWAWTF
                     FMIAYTTLIGYGGAVLVYQGGRMLGLG"
BASE COUNT          342 a          553 c          654 g          527 t
ORIGIN      
        1 tggctgttgt cgtggcggat gcgtccaacc tggaacgcag cctctatctt gccgtgcaga
       61 tgcttgagat cggcactccg ctcgtccttg cgctgaacat gatggacatg gcggcggaga
      121 ggggaattat gatcgacgtg gaacgccttt cggcgcttct tggtattccc gtcgtgccga
      181 ccgtcgcacg gaagaaggaa ggaatagaag aactcaaaga gagaattact cgttctcttc
      241 gggagaactc ggcgccctct ctttctttcg ccctgccgta cggagaacgc ctgaatccct
      301 tgttcgaagc gcttgaaacg tttctggggg agcgccagga actcattccg gggctttccg
      361 cacgttccgc ggcggtgaag ttcgtcgagg gcgacgttgc ggttcttgcg gccgcggaga
      421 gcagcggcgt tcgcaacagc atggacgaac tccttgccga gaaggggcgt aaggtggagg
      481 aggagttggg atacgatctc caaactgccg tgatcgaaag acggtggggc tttgtttcgg
      541 gcgtgacggc ggaatcggta caacgcgacc tctcgctcaa agcgcgcctg accctttcgg
      601 atcgcatcga ccgcgtggtg acttcgcgat ctctagggtt gccgctcttc tttctcgtca
      661 catgggcaat attccgcatg acctacgcct taggcgaccc gttggtggag ctcctggaag
      721 gagtgttcga atccttcggg gaatggacgg gcgagttttt cgccggcgcc gggttttcgg
      781 agatcgtctc atccttcgta caggacgggc tcattggggg cgtcggctcg gtcgtcgtct
      841 tcttccctca tatcttcatc cttttcgcgt tcatcgcatt gctggaggat tcggggtata
      901 tggcgcgggg tgcgtttgtg atggacaggg ttatgcacct gatggggctg cacggcaaga
      961 gtttcatccc catgctcatg ggttttgggt gcaacgtgcc ttcgatgatg gcgacgcgta
     1021 ttcttgaaca gcccagggac agaatgatca cgctgcttgt gctgcctttt atgagctgtt
     1081 cggcccgtct tcccgttttc gtgctctttt cgggcatatt cttcggagcg catgccggga
     1141 cggctgtctt ttccctgtat gttctcggaa tcatcgtagc ggtgctcgcg gccaaggtac
     1201 tagggagcac gctctttaaa ggggagtcgt cgcagctggt catggagttg cctccgtacc
     1261 atgttccctc gctttcgatg gtgctgcgtc atgcttggga gcggggattt ctctttctcc
     1321 ggaaagcggg aacattcatc cttctttcgg tgatcgccgt atgggtcctc gcaagcttcc
     1381 cgtggggcgt ggagtacggt tcgtccgaga gtatcgtcgg gcgtatcggc cagatactcg
     1441 ccccggtttt cgctcccgcg ggctttggtt tctggcaggc tgctgtggcg ctcttcttcg
     1501 gtttcctcgc aaaagaggtc gtcgtcggaa cgctgggggc gcttctcgga gcaggggagg
     1561 cggggcttgc cgcggcgctg ccgggcctct tcacgccgct ctccgcgtat gccttcctcg
     1621 tgatgacgct cctgtacgtg ccgtgcgtgg ccgctgtcgc cgccttccgc agggagaccg
     1681 gaagctgggc ctggacattc ttcatgatcg cctatacgac gctgataggc tacggcggcg
     1741 cggtactggt ctatcagggc gggagaatgc tgggtctggg gtaagtattt cccccatcgg
     1801 catttttact gaaaaaaact gcggcggccc cccttttcag aagaagcggg ggtccgccgc
     1861 gtttcaatat ggtacaatac gcgatgtctg aaaaagggtt ccggagtgtc tccggaattc
     1921 tttggcgtag cagtgaaagg aagatgtttt catgccgttt tctttttcat cgatctccct
     1981 ggagagagcg gatgaataca gacagctcta ctcgcagtct ccgcggaaat ccgcgtggta
     2041 ttctttcgga agtctctggg cgtggcgaaa catcta
//
LOCUS       DAOSWW010000486         2076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43939_length_2076_cov_2.750124, whole genome shotgun sequence.
ACCESSION   DAOSWW010000486 DAOSWW010000000
VERSION     DAOSWW010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43939_length_2076_cov_2.750124"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PK442_09540"
     CDS             <1..1322
                     /locus_tag="PK442_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO87936.1"
                     /translation="VRLEQVLVNLTNNAVKFTERGSVTLSVSFDPLKKNEGALTFRVT
                     DTGIGIPPEERKRVFEPFYQADNSNTRKYGGIGLGLSISQRILQMMGGSLEIHSVPGE
                     GSSFFFTLRLAYDAEEARSPAATPLPEEKRGFPHSRRKDTPVILLAEDERLNMKMLTI
                     MLSKLAPFSSVVQAEDGEKAVALFREHRPDLVLMDLQMPVIDGLQATAEIRAIEAKEC
                     REKERCCIVALTADVLPETRGECLAGGMDDYLSKPVRREEIMAVLERCFGMNGGGERD
                     FRDAPAEDAAWNRPLMLDVLDGDEALADEIASEFLKETPQMLAALSDALAERDMEKLR
                     RRAHTIKGIAGNVGGEALRSAAFDVEKTARLGDREKAVLLVVRLKDEFERLRKAMDNH
                     LKTRTSLEAKTETNPQPEETPAVADEERRRNAPHKSTTDTATFQGEQ"
     gene            1319..>2076
                     /locus_tag="PK442_09545"
     CDS             1319..>2076
                     /locus_tag="PK442_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO87937.1"
                     /translation="MNEEQRTILLVEDTRTNALAVERQLRKAGWGVLHAQTGEEAIRM
                     VCDEGAEIDLILMDIELGSGMDGSQTAREILKRKELPVVFLSSYTEAEVLERIEKIAS
                     YGYVAKNSGGAVLDASIKMAFRLHEANMRTARERNILRDVLESTLSGFWEMYFKSDAA
                     FLSPKFRNLLGYGEEELPGTSMLCHRIVHPEDLPGTLHLLQEHIQSKGKTPFSNEIRY
                     VRKDGLILWAICAGRVGEWAEDGSPVTMVGCYID"
BASE COUNT          491 a          592 c          639 g          354 t
ORIGIN      
        1 ccgtgcgtct ggagcaggtg ctcgtcaatc tgacgaacaa cgcggtcaaa ttcacggagc
       61 ggggaagcgt cacgctctcg gtcagcttcg atcctctgaa gaaaaacgaa ggcgccctca
      121 ccttccgcgt gacggatact ggcatcggaa tccctccgga agagcgaaaa cgcgtcttcg
      181 aacccttcta ccaggccgac aactccaata cgcgcaagta cggagggatc gggctcggcc
      241 tctccatcag ccaacgtatt ctgcagatga tggggggttc gctcgaaata cacagcgtcc
      301 ccggagaggg gagcagcttc ttcttcacgc tgcggctggc atacgacgcg gaggaagcgc
      361 gctctccggc ggcgacgccc cttccggaag aaaaacgggg atttccccac tcgcggagaa
      421 aagacacccc ggtgatcctg ctcgccgagg acgaacggct gaatatgaag atgctcacca
      481 tcatgctgtc gaagcttgct cctttctcct ccgtagtaca ggccgaagac ggagaaaaag
      541 cagtcgccct cttccgcgaa caccggccgg acctcgtcct gatggatctt cagatgccag
      601 tgatcgacgg cttgcaagca acggcggaga tacgcgcgat tgaagcgaag gaatgccgcg
      661 aaaaagagcg ctgctgcatc gtcgccctca cagcggacgt tctgccggaa accagagggg
      721 agtgtctcgc gggcggtatg gacgactatc tgtcgaaacc cgtacgcaga gaagagatca
      781 tggccgtcct cgaacgatgc ttcggaatga atggaggcgg agaacgagac ttccgggacg
      841 cacccgccga ggacgcggcc tggaacagac cgctcatgct ggatgtgctc gacggcgacg
      901 aagcgctggc cgatgaaatt gcgtcggagt ttttgaagga aacgccgcaa atgctggccg
      961 ctctcagcga tgcgctggcg gagagagata tggagaaact cagaaggcgc gcccatacga
     1021 tcaaggggat cgccggaaat gtaggcggag aggctctgcg ctccgccgcc ttcgacgtgg
     1081 aaaaaacggc gcgcctgggg gatcgggaga aagccgtcct tcttgttgta aggttgaagg
     1141 acgaattcga gcggttgcgg aaggcaatgg acaaccatct gaagacgcgg acttccttgg
     1201 aagcgaagac cgaaacaaat ccccaaccag aagaaacgcc tgcggtagcc gatgaagaac
     1261 gccgccggaa cgcgccgcac aaatctacta ctgacaccgc aacttttcag ggggaacaat
     1321 gaacgaagag caaagaacga ttctcctcgt cgaggacacg cggaccaacg cccttgcggt
     1381 ggaacggcag ctccgcaagg ccggatgggg cgtgctccat gcgcagacgg gcgaagaggc
     1441 catccgcatg gtgtgcgacg agggtgcgga gatcgacctg atcctaatgg acatcgaact
     1501 cggctccggc atggacgggt cgcaaaccgc ccgtgagatt ttgaagcgga aagagcttcc
     1561 cgtcgttttc ctctcctcct acaccgaggc ggaggtgctg gagcggatcg aaaagatcgc
     1621 ctcgtacggc tacgtcgcca aaaactcggg aggggccgtg ctcgacgcct ccatcaagat
     1681 ggccttcagg ctccacgagg cgaacatgcg gacggcgcgc gagcgaaaca tcctgagaga
     1741 cgtccttgag tccaccctct ccggtttctg ggagatgtac ttcaaaagcg acgcggcgtt
     1801 tctgagtccc aagttccgga atctgctggg gtacggagag gaggagctgc ccggaacatc
     1861 gatgctctgc catcggatcg tccaccctga ggatttaccc ggcacgctcc atctccttca
     1921 ggagcacata caaagcaaag gaaaaactcc tttctccaat gaaattcggt atgtgcgcaa
     1981 ggacggcttg atcctctggg cgatctgcgc cggtcgggtg ggcgaatggg ccgaagacgg
     2041 ctctcccgtc acaatggtgg gatgttacat cgacat
//
LOCUS       DAOSWW010000487         2075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_43953_length_2075_cov_3.366337, whole genome shotgun sequence.
ACCESSION   DAOSWW010000487 DAOSWW010000000
VERSION     DAOSWW010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43953_length_2075_cov_3.366337"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1268
                     /locus_tag="PK442_09550"
     CDS             96..1268
                     /locus_tag="PK442_09550"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO87938.1"
                     /translation="MLSQKKSRRVRFVLSLSMFCAMLGIGIIAPVIPLYAKSLGASSV
                     AIGFIIGSFSFSRIWGMLVSGELAERMDRKKLLLGGLFVYAAASVAYTFSFSTEMLIF
                     IRTLHGIGSAMVVPITMAIAADIAPKGEEGAIFGSLQAALFLGVGSGPLLSGFLADTF
                     GFYAPFYAMTALTLLALFLVLALFPSSPPKNTVKEEVGALKAFKGIISDPEMLNVFIF
                     QFSSAMCRGVLVMVIPLFASQFDLSLSRIGIIVSLNSISTGILQNVSGKMADNVHKNT
                     LIIIGGVISAVTLIGLPNLTTPLSLSVASVAFGVGHAFASPSLAAIAAARGKNFGSGR
                     TMGLFNIAFSIGMTIGPIMVGSLLDITGNGIPFYILSAFLAASALPFFPVFKRGVC"
     gene            complement(1317..>2075)
                     /locus_tag="PK442_09555"
     CDS             complement(1317..>2075)
                     /locus_tag="PK442_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87939.1"
                     /translation="VWTTTPWLALVSRFIGGVFVAVRPIEFWAEDGQVVRKVQNRAIP
                     KDSRIIALDTEEASRLGLNQKEWMSLALLYRTAPFDKKRLDYFFADPSSLPFIAQKVF
                     TVLSHFDPANYPYYQRRLSEFQTRLDSTVIVGRRLLSGSAFIYIGGDFSGMLTAAGCS
                     MESRDESLNTLWEQGKELDLLFALLEDALKRRIPVIVDTSSKKLKEALRNNKEILFLE
                     KPALDQDILLFFHDQYIFLWNRLAALRQLRTPQK"
BASE COUNT          479 a          460 c          508 g          628 t
ORIGIN      
        1 gaacttgaaa ttgtagacaa tatatagtat catagccaag gaatattttc tttccatcgt
       61 gtttatcgag gaaagatagg ggatgtgttt ccttgttgct atcacaaaaa aagtcgcgga
      121 gagtccggtt tgtactgagt ctttcaatgt tttgtgctat gttaggtata ggaattattg
      181 caccggtcat acctctttat gcgaagtctc ttggcgcaag cagcgtcgcg attggattca
      241 taattggctc tttttctttt tcccgtattt gggggatgct tgtgtcggga gaactcgctg
      301 aacgtatgga ccggaaaaag cttcttttag gcggtctttt tgtgtatgcg gcggcttcgg
      361 ttgcctatac gttttctttt tcaaccgaga tgctgatttt cataaggacg ttgcacggga
      421 taggttctgc gatggtagtg ccgattacga tggcgatcgc ggccgatatt gctccaaagg
      481 gcgaagaagg agcaattttc gggtctcttc aggcggcctt gtttcttggg gttggttccg
      541 gtccgcttct cagcggtttc ttagccgaca cttttgggtt ctatgctccg ttttacgcca
      601 tgacagcact cactctcctc gccctttttc tcgttctggc cctcttcccc tcttccccac
      661 cgaaaaatac agtcaaggaa gaagtgggcg ccttgaaagc ttttaagggc atcatttctg
      721 atccggagat gctcaacgta tttattttcc agttttcttc ggcgatgtgc cgtggtgttc
      781 tcgtaatggt tattcctctt tttgcttcgc agtttgacct ttccttgtcg aggatcggca
      841 ttattgtttc actgaattct atttcgaccg gtattctaca gaatgtatcc ggtaaaatgg
      901 cggataatgt gcataaaaat accctgatca ttattggtgg agtgatttca gctgtgacgc
      961 tgataggact ccctaatttg acgacacctc tcagcctctc tgtagcaagc gtggctttcg
     1021 gagtcgggca tgcgtttgct tccccttcgc ttgctgcgat tgcagcggct aggggtaaaa
     1081 acttcggttc agggaggacg atgggccttt ttaatattgc attcagcata ggcatgacga
     1141 ttggccctat aatggtcggc tctttgttgg acattaccgg aaacggcata cctttttata
     1201 tcctaagcgc ttttctggca gcttctgcgc ttccattttt tccggttttt aaacgcggcg
     1261 tgtgctaaaa attaaacaaa gagacgggag aagtcctccc gttcagaatg gtttttttat
     1321 ttctgcgggg tacggagttg tctcagagcg gcaagtctgt tccacaagaa aatatactga
     1381 tcgtgaaaga aaagcagtat atcctggtca agggcgggtt tctccaggaa aagtatctct
     1441 ttgttgttgc gtagcgcttc tttaagcttc ttggaagatg tatcgacgat tactggaatt
     1501 ctccttttga gagcatcctc aaggagagcg aaaagaaggt cgagttcttt tccttgctcc
     1561 caaagtgtat tcaatgactc atcccttgat tccatcgaac atccagctgc agtcagcatc
     1621 ccggaaaaat cacccccaat atagataaag gctgatccgc tcaaaagcct tcttcccacg
     1681 atgaccgtgc tgtcgagacg cgtctgaaat tctgaaagcc gacgttggta gtaggggtag
     1741 tttgccgggt cgaaatgtga gaggaccgtg aaaaccttct gtgctatgaa tggaagcgaa
     1801 gacgggtcag caaagaaata atccaaacgt tttttgtcaa aaggagctgt acggtaaaga
     1861 agtgcaagcg acatccactc tttttggttc aatccaagcc gggaagcttc ttcagtatca
     1921 agcgcaatga tacggctgtc tttgggtatt gcacggtttt gtactttgcg tacaacttga
     1981 ccgtcttcag cccaaaactc aatcggacga accgctacaa aaacgcctcc gataaagcgg
     2041 cttacgagag caagccaagg cgtcgtcgtc catac
//
LOCUS       DAOSWW010000488         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44027_length_2073_cov_3.014866, whole genome shotgun sequence.
ACCESSION   DAOSWW010000488 DAOSWW010000000
VERSION     DAOSWW010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44027_length_2073_cov_3.014866"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK442_09560"
     CDS             <1..241
                     /locus_tag="PK442_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with RbsBCD acts to import ribose into the cell;
                     RbsA contains 2 ATP-binding domain; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HOO87940.1"
                     /translation="LILDEPTRGIDAGARGDVYRIIDALKAEGLAVLLISSDMEEILA
                     LSDRVVTMYNGAINREFGPGEIALDGLMAASFGVC"
     gene            243..1259
                     /locus_tag="PK442_09565"
     CDS             243..1259
                     /locus_tag="PK442_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87941.1"
                     /translation="MLPSFRMKREFNALLFAVLLFVGIGYVNPVFLTVQNIALTLQGS
                     VMYIFLAVGMTFVILTGDIDVSVGAVLGMSAAVAATLVRDGYPLWAVVGGALLVGGGT
                     GLLNGIGVTFLRIPAIVMTLGTMGILRGAMFIYTDGKWVENLPDFFKQACQICVYGVN
                     LFVLAAIAVVLLMQLYLSTARRGKYFAAVGDNLDGAVLVGVPVVRIRIAAFIISGLCS
                     ALAGLVYTSQIGFVGNVAGNGTEMTAIAACVIGGVSLAGGTGSVAGALFGAVIMNTIN
                     SALVFLKVPAYWNNTISGLLLLTMVLADSLLHRYLEEKARRKRLNARFARAAERRACD
                     DARA"
     gene            1246..>2073
                     /locus_tag="PK442_09570"
     CDS             1246..>2073
                     /locus_tag="PK442_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="with IsrABC is involved with autoinducer 2 import;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 import system permease LsrD"
                     /protein_id="HOO87942.1"
                     /translation="MHVLKRYLLRWESVLILLLIAEIAIFGRINPRFLNTRVLLYSVN
                     DFVCVSIISLFMAFVVITGGIDISGGSIIGLTSVVVGVLWKQAGLDIVSASFAGLATG
                     ALCGAFNGFLVAFAGVQAMVVTLGGMMFFSGLALVLVGVSGVSAFEGISGFPDGFMEI
                     AGGDLWGVPNPVLIFAALFAVACVLLHCTNYGRRVFLVGINPNAARYSGLGVRGVVMS
                     TYVLSGLGASIAGIVLTSYLGGARSDLGAELTLPIVTAVVLGGVSITGGRGTVVGVAI
                     "
BASE COUNT          286 a          675 c          702 g          410 t
ORIGIN      
        1 gctcatcctc gacgaaccca cccgcggcat cgacgccggg gcgcgcgggg acgtctaccg
       61 catcatcgac gcgctcaagg ccgaggggct tgccgtgctc ctcatctcct ccgacatgga
      121 ggagatcctc gcgctgagcg accgtgtggt cacgatgtac aacggagcca tcaaccgcga
      181 attcgggccc ggcgagatcg cgctcgacgg cctgatggcc gcctcgttcg gagtgtgctg
      241 acatgctgcc ttcgttcaga atgaaacgcg agttcaacgc gctgctcttc gcggtgctcc
      301 tcttcgtcgg gatagggtac gtcaacccgg tcttcctcac ggtccaaaat atcgcgctga
      361 cgctgcaggg gagcgtgatg tacatcttcc tcgcagtggg gatgaccttc gtgatcctca
      421 ccggcgacat cgacgtgtcg gtcggggccg ttctcgggat gtccgcagcg gtggctgcca
      481 cgctggtgcg cgacggatat ccgctgtggg ccgtcgtcgg cggggcgctg ctcgtcggcg
      541 gcggaaccgg gctgctgaac ggcatcgggg tcaccttcct ccgcattccg gcgatcgtga
      601 tgacgctggg cacgatgggg attctgcggg gcgcgatgtt catctatacc gacggcaagt
      661 gggtggagaa ccttcccgac ttcttcaagc aggcgtgcca gatctgcgtc tacggagtca
      721 acctgttcgt cctcgcggcg atcgccgtag tgcttctcat gcagctctac ctctcgacgg
      781 cccgccgggg caagtacttc gccgccgtcg gcgacaatct cgacggggcg gtgctcgtcg
      841 gcgtgccggt cgtgcggatc cgcatcgccg ctttcatcat ctccggcctc tgctccgccc
      901 tcgcgggact ggtatacacc agccagatag gtttcgtggg caacgtcgcg ggcaacggga
      961 cggagatgac ggcaatcgcg gcctgcgtca tcgggggcgt cagcctcgcc ggggggacgg
     1021 ggtcggttgc cggagcgctc ttcggcgcgg tcattatgaa cacgatcaac tccgcgctcg
     1081 tcttcctcaa ggtgccggcc tactggaaca acacgatctc cgggctgctg ctgctgacga
     1141 tggtgctcgc ggactccctg ctgcaccgct acctggagga gaaggcgcgg cgcaagcggc
     1201 tgaacgcgcg gttcgcgcgc gccgcggaga ggagggcgtg cgacgatgca cgtgcttaaa
     1261 cgataccttc tgcgctggga gagcgtgctc atcctgctgc tgatcgcgga gatcgcgatc
     1321 ttcgggcgca tcaacccccg cttcctcaac acgcgggtgc tgctgtacag cgtcaacgac
     1381 ttcgtctgcg tcagcatcat ctcgctcttc atggcgttcg tcgtcatcac cggagggatc
     1441 gacatctccg gcggctcgat catcgggctg acctcggtcg tcgtcggcgt cctctggaag
     1501 caggcggggc tggacatcgt ttcggcgtcg ttcgccgggc tcgcgaccgg ggcgctctgc
     1561 ggggcgttca acggctttct ggtggccttc gcgggggtgc aggcgatggt ggtcaccctc
     1621 ggcggcatga tgttcttctc gggactcgcg ctcgtgctcg tcggcgtgtc gggcgtcagc
     1681 gcgttcgagg ggatcagcgg gttcccggac ggcttcatgg agatcgcggg cggcgacctg
     1741 tggggcgttc cgaaccccgt gctcatcttc gccgcgctct tcgcggtcgc atgcgtgctg
     1801 ctccactgca cgaactacgg ccgccgcgtc tttctggtag gaatcaaccc gaacgcggcc
     1861 cgctactcgg ggctcggcgt gcggggcgtc gtgatgagca catacgtcct ctccggcctc
     1921 ggcgcctcca tcgccggaat cgtgctcacc tcgtacctgg gcggagcgcg ctccgacctt
     1981 ggcgcggagc tgacgctgcc catcgtcacc gcggtcgttc tcggcggcgt ctccatcacg
     2041 gggggcaggg ggacggtcgt cggcgtggcc atc
//
LOCUS       DAOSWW010000489         2067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44254_length_2067_cov_3.516899, whole genome shotgun sequence.
ACCESSION   DAOSWW010000489 DAOSWW010000000
VERSION     DAOSWW010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44254_length_2067_cov_3.516899"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="PK442_09575"
     CDS             <1..1565
                     /locus_tag="PK442_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO87943.1"
                     /translation="ICLKRSDALALISKERSVTYAGLCDMISRYSALISSTPCTKVAI
                     FSENRPEWVYIMYAGWKSGYTVVPIDYMSPADEVAYILADCKPEKIFCSRRKFPELEK
                     ALLSLSYAPEILVFEECLLPPGANASGDHFLERDDDDTALIIYTSGTTGSPKGAMISF
                     ANIAANVRAVSEAAPIFTPESRVLVLLPIHHVLPLGGTILAPLFVGACCVFSPSIGSR
                     DILETLQHNGVTVVVAVPRFYSMLIKGIREKIQESFTARALFALAKKIDHPGLSKFLF
                     KKVHERFGGKIRFLVCGGAAIDDDVASDFKALGFDMLTGYGMTETAPLISFTHPGTLR
                     IGASGYVCPAVEVRIVDGEITVRGRHVMKGYYNRPEETAAMIRDGWLHTGDLGFVDED
                     GFLFVTGRKKDIIVLSNGKNINPEEIEAKLLSGFNAVKEVAVCLYNGVLHAIILPDFE
                     KIAESGKRPSEEFFRHEVIGEYNHRVASTKKIMKFTLVQDELPKTRLGKIRRFQLPEL
                     LALIAEAESKAN"
     gene            complement(1686..>2067)
                     /gene="glgC"
                     /locus_tag="PK442_09580"
     CDS             complement(1686..>2067)
                     /gene="glgC"
                     /locus_tag="PK442_09580"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710787.1"
                     /note="catalyzes the formation of ADP-glucose and
                     diphosphate from ATP and alpha-D-glucose 1-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOO87944.1"
                     /translation="SYVCPVKGFSCYVQSTLSAEGSRVLGSRVESSVLSRNCVIMPGS
                     DIEQSIIGEGVVVGENCRLKKVIIDSHNVIPPNTEIGFDPEHDRERYAVDPNSGIITI
                     GMPKMQLRKSIEQPKEGFDWSSFS"
BASE COUNT          452 a          540 c          585 g          490 t
ORIGIN      
        1 tgatatgcct gaaacgcagt gacgcactcg cgcttatttc aaaagagcgc tcggtaacct
       61 acgccgggct ttgcgatatg atctcccggt actccgcgct catatcgagc acgccgtgca
      121 cgaaggtcgc tattttctcg gagaaccggc cggagtgggt gtatatcatg tatgccggct
      181 ggaagagcgg ttataccgtg gtgccgatag actacatgtc cccggccgat gaggtggcgt
      241 atatcctcgc cgactgtaaa cccgagaaaa ttttttgttc ccgcaggaaa tttcctgaac
      301 tcgaaaaagc gcttctctcc ctctcctacg ctccagaaat tttggtattt gaagaatgtc
      361 ttcttcctcc gggagcgaat gcctccggag atcatttcct cgaacgcgac gacgatgata
      421 ctgctctgat aatttataca tcgggcacca ccggaagccc gaagggcgcc atgatctcct
      481 tcgccaatat tgcggcgaac gtcagggcgg tgagcgaagc cgctccgatt ttcacccctg
      541 aatcgcgggt tctggtgttg ctgcccatcc atcacgtgct tcctctggga gggacaatcc
      601 tcgcccctct cttcgtggga gcgtgctgcg tcttttcacc ttcgataggt tctagggata
      661 tactggagac gctccagcac aacggagtca cagtggtcgt cgctgttccg cgtttctaca
      721 gcatgctcat aaagggcatc cgggagaaga tacaggaaag ttttaccgcg cgggctcttt
      781 tcgctctggc aaaaaaaatt gatcatccgg ggctctccaa gtttctgttc aaaaaggtcc
      841 atgagcgttt tggaggaaaa atccgttttc tggtctgcgg cggcgcggcg atcgacgacg
      901 atgttgcaag tgactttaaa gcgcttggat tcgacatgct taccggctac gggatgacgg
      961 agactgcgcc gcttatttcg ttcacgcatc cgggaacctt gaggatcggg gcgagcgggt
     1021 acgtctgtcc cgccgtcgaa gtgcgtatcg tcgacggtga aatcaccgtc aggggacgtc
     1081 atgtaatgaa ggggtactac aaccgccctg aagaaacagc cgccatgatc cgcgacggct
     1141 ggctgcacac aggagatctt ggattcgtgg atgaggatgg ctttcttttc gttaccggcc
     1201 gaaagaagga tattattgtg ctttccaacg ggaagaatat caatcccgaa gagatcgagg
     1261 cgaagctgct ctccggattt aatgcggtga aggaggttgc ggtctgtctt tataacgggg
     1321 tgctgcacgc catcattctc cctgattttg aaaaaatcgc agaaagcggc aagcggccgt
     1381 ccgaagagtt ctttcgtcac gaggtgatcg gcgagtacaa ccatcgggtt gcgtcgacga
     1441 agaagataat gaagtttacc ctggtacagg atgaactgcc gaaaacacgc ctcggaaaga
     1501 tcaggaggtt tcagctgccg gagctgctcg ccctgattgc agaggcggag agcaaggcga
     1561 actgataccg gcgcaccgga ataatttccg gcttcagcgt tcccggagag gaagggtgat
     1621 cctgtatttg ccaaatatcg ggaagaacag gggggcgttt gaacgccccc ctgttctgtc
     1681 tgctgttaac tgaagcttga ccagtcgaag ccttccttcg gctgttcgat ggacttgcga
     1741 agctgcatct tcggcatgcc gatggtgatg atgcctgaat tcgggtccac ggcgtaccgt
     1801 tctctgtcat gctccggatc gaatcctatc tcggtgttgg gcgggatcac gttgtggctg
     1861 tcgatgatga cctttttaag acggcagttt tccccgacga ccactccctc gccgatgatc
     1921 gattgttcaa tatccgagcc cggcataatc acgcagttgc gggacagcac ggacgactcg
     1981 actctggatc caaggacgcg gcttccttcc gccgaaaggg tgctttgaac gtagcaggag
     2041 aatcctttca ccgggcagac gtacgac
//
LOCUS       DAOSWW010000490         2064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44363_length_2064_cov_3.629667, whole genome shotgun sequence.
ACCESSION   DAOSWW010000490 DAOSWW010000000
VERSION     DAOSWW010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44363_length_2064_cov_3.629667"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PK442_09585"
     CDS             complement(<1..405)
                     /locus_tag="PK442_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87945.1"
                     /translation="MKMGVFNVSLLLFFLLCVLSVPSEAVSEYSILRTDMYPAGARLV
                     FSIPASEKVHFQMPGAFDSASVRPLPSEGVEVLSFEAVDVSRSEWIPPVLEKLFTSIR
                     EKERLQATLEGKISAIENSLALLSGPLPKELKG"
     gene            580..1671
                     /gene="ychF"
                     /locus_tag="PK442_09590"
     CDS             580..1671
                     /gene="ychF"
                     /locus_tag="PK442_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOO87946.1"
                     /translation="MLKCGIIGLPLSGKSTVFNVVTRAGAEVKPYASGKTDPNRAMVN
                     VPDDRFDHLARLFEPKKVTPATVEFVDLAGLSRNASKGEGVGNAFLSFVSESDALVHV
                     VRLFENPDVPHPENSIDPVRDWNIVEMELIFRDLGVIENRISRLQAKKKNIPSETEEK
                     ALLERCQACLLEERPLRELGLSPDELRLLSGFAFVTLKPELIVANLDETQTDDSLPLL
                     NALRELAKTKGIRLLRIFGKMEMELTELPEEEQKEFMGELGIEESGRNRLISEAYALL
                     GLLSFFTTGKDEVRAWTLVKGANAVDAAGAIHSDLARGFIRAQVVSYEDFISHEQSLA
                     KCREKGVLRLEGKDYVVNDGDMIEIRFNV"
     gene            1720..>2064
                     /locus_tag="PK442_09595"
     CDS             1720..>2064
                     /locus_tag="PK442_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87947.1"
                     /translation="MAMPMQVSLDEILSMLLARVDSLAINDENSKSKFNIVARVLYKK
                     GILTDADIVESIKEEHRMLKELGAIAEEPSDDIVETIADGILQWIKGDVESLKKGMEE
                     YERKMRDLAAQEA"
BASE COUNT          569 a          496 c          540 g          459 t
ORIGIN      
        1 gccttttaat tccttgggaa gagggccgga aagaagcgcc agcgagttct cgatggccga
       61 aatcttgccc tccagagtcg cttgtagtcg ttctttttca cgaatagacg taaaaagttt
      121 ctccagtacg ggaggaatcc attcggaacg cgatacgtca acggcttcga aagagagaac
      181 ttccacccct tcagagggga gcgggcgaac cgaagcgctg tcgaaagctc cgggcatctg
      241 gaaatgcacc ttttccgaag ccggaataga aaagaccaac cgtgcgccgg cagggtacat
      301 atctgtacgg agaatcgaat actcagagac agcctcggag ggaacgctta ggacgcacaa
      361 cagaaaaaaa agcaaaagcg atacattgaa gacgcccatc ttcatctcga aaaacctcct
      421 tgatcaaggt ataaaagctt gcaatcaatg ataacagaaa tttgaaaaac cggattgact
      481 cttttttaaa gtacacgcga ttcttccctt tcggcgtgga aaaggtataa tgcatcacgg
      541 gactgaaaaa aactgtctcg caacaaagga ggaagaaaaa tgctcaaatg tggtattatc
      601 ggccttccgc tgagcggaaa gagcacggtt ttcaacgtag tcacacgtgc cggcgccgaa
      661 gttaagcctt atgcaagcgg gaagacggat cccaacagag caatggtcaa cgtccccgat
      721 gatcgtttcg accacctggc acgcttgttc gagcccaaaa aagtcactcc ggcgaccgtg
      781 gaattcgtcg atcttgcagg tctttcgcgc aacgccagca aaggagaagg ggtaggaaat
      841 gcttttctct cttttgtctc cgaatctgac gcattggtcc acgttgtccg cctttttgag
      901 aatcccgacg tcccgcatcc ggaaaattcc atcgatcctg taagagactg gaatatcgtc
      961 gagatggaat taatcttccg cgaccttggc gttattgaaa acaggatttc ccgcctgcag
     1021 gcaaagaaaa aaaacattcc ttccgaaacg gaggaaaaag cgctgcttga acgctgccaa
     1081 gcctgcctcc ttgaggagcg ccctttgaga gaactcgggc tttcgcccga tgaattgcgt
     1141 cttctcagcg gttttgcttt tgtaacgctg aagccggaac tcatcgtcgc gaatctcgac
     1201 gaaacccaga ccgatgattc gcttcctcta ctcaacgcac tccgcgagct ggcgaagaca
     1261 aaaggcatcc gtttactcag gatcttcggg aaaatggaga tggagcttac tgaacttccc
     1321 gaggaagagc agaaggagtt tatgggcgaa ctcggcatag aagaatcggg ccggaaccgc
     1381 cttatctccg aagcctacgc ccttcttggg cttctctcgt ttttcacgac gggaaaggat
     1441 gaggtcaggg catggacgct tgtaaaaggc gcaaatgcgg ttgacgctgc aggagcgatc
     1501 cattcggatc ttgcacgtgg ttttattcgg gcgcaggtcg tctcctacga agattttatt
     1561 tctcacgagc aatctttagc aaaatgccgg gaaaagggcg tgcttcgcct tgagggcaaa
     1621 gactacgtcg taaatgacgg cgatatgata gaaatccgct tcaatgtcta atgtgataca
     1681 atcagtactc taattttcct agaaaggggc gcttttccaa tggccatgcc aatgcaggta
     1741 agtctcgatg aaattctctc aatgctcctc gccagggtgg atagccttgc gatcaacgat
     1801 gagaattcaa agagcaagtt caatatcgtc gcacgcgttt tgtataaaaa ggggattctc
     1861 acggacgcgg atatagtgga gtcgatcaaa gaagagcacc gcatgctgaa ggagcttggc
     1921 gccatagccg aagagccttc cgacgacatc gtggagacca tcgccgacgg aattctccag
     1981 tggatcaaag gtgatgttga atctttgaag aaggggatgg aggagtacga gcgaaagatg
     2041 agggatcttg cagctcaaga ggcc
//
LOCUS       DAOSWW010000491         2064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44369_length_2064_cov_3.249378, whole genome shotgun sequence.
ACCESSION   DAOSWW010000491 DAOSWW010000000
VERSION     DAOSWW010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44369_length_2064_cov_3.249378"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..>2064
                     /locus_tag="PK442_09600"
     CDS             272..>2064
                     /locus_tag="PK442_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOO87948.1"
                     /translation="MINALRSLSLPRRGIIDTEDVHQKETPVEKTETENESGLPSRFV
                     LVRPPSHGIPADVLQRIEETQLSEERLPQEDTEGFEEEKNGALFGFFKRKRKTLPEDE
                     PLLTENSIVALTPHEARSEALPEQPDAALPEVFETHSDREEMSEVDDTDTDTDIEMEE
                     DVFLLRRPEENISGQGLQTELPEDDAVVAKIEQDSEDVLTDPHALPDIEFHVVEEEGR
                     VRSGTFPPPPEIFGPRQEMETSDDNETVREQASSIITTLADFGVSAEMADIVIGPTVI
                     QFQLQLAPGIKVSKVAGLSNDLAVALTVPSLRVEAPIPGKPYVGVEIPNPKRKGIALR
                     TILESRVFLDSENDLPLAMGVRVDSRPLVVGLEDLPHLLVAGTTGSGKSVFVTSCITG
                     LCTHRSPEELRLILIDPKRVELSIYEHLPHVLAKPVVSPKKAVQALAWAVREMEHRYE
                     VFARARVRNLKSYNQNVLPKAKLPHIVIVVDELADLMFTAQKDVEDYICRLAQMARAT
                     GIHLVLATQRPSVNVITGLIKANIPARVAFTLPSQTDSRTIIDVSGAERLLGKGDMLF
                     VSSKYPRPLRLQAPFIDDGKSLEIIEYLGNI"
BASE COUNT          474 a          538 c          548 g          504 t
ORIGIN      
        1 gacaggagcg aaaggtcttc tctcccaggc tctgggtacg tttcttcttt ttttatgtac
       61 tgcccttctg ttcgggctct ttcagacaac ccgtattttt caaggttttt cgttatttac
      121 tcccgggtat ctaggtcaag gtctggcggt ctttcttacc agggagatag gggcccttgg
      181 aacaacgctc cttgccgtgg cgttgcttat tctttctgca accctctatg gctttattca
      241 tccggcttct gtaatatcca ggatttcaaa gatgatcaat gctcttcgtt ctctttcgtt
      301 accgagacgc ggcatcattg atactgaaga tgtacaccaa aaagaaaccc ccgttgagaa
      361 gacggaaacg gaaaacgaat ccgggcttcc ctcccgtttt gttctggtgc gtcccccctc
      421 tcacggtatt cctgcggatg tgctgcagcg aatagaagaa acgcagcttt cagaagagcg
      481 gcttcctcag gaggacaccg aaggattcga agaagaaaag aacggggcgc tttttggttt
      541 tttcaagcgg aaaagaaaaa cgctccctga ggatgagcct cttcttactg aaaactcgat
      601 agttgcgctc actccacacg aagcgaggag cgaagccctc cccgagcagc ccgatgctgc
      661 gcttcctgaa gttttcgaaa ctcattcaga ccgggaagaa atgagcgaag tggacgatac
      721 cgacaccgac acagatattg agatggagga ggatgtcttc ctcctgagac gaccggagga
      781 gaacatatca ggacaaggac tgcaaacgga gcttccggag gacgatgcag tcgtcgcgaa
      841 aattgagcaa gacagcgaag atgttctcac agatccgcac gctctgcccg atattgagtt
      901 tcatgtggtg gaagaagaag gtcgtgtccg gagcggcaca ttccctcctc cgccggaaat
      961 tttcggtccc aggcaggaga tggagacatc ggatgataac gaaaccgttc gagaacaagc
     1021 ttcgtcgatt atcaccacgc tcgcggattt tggcgtaagt gcggagatgg cggatatcgt
     1081 catcggtccg acggttatcc agttccaact acaacttgct ccgggaataa aggtgagcaa
     1141 ggttgccggt ttgagcaacg atcttgctgt tgcgctcacc gtgccttcgc tccgtgtaga
     1201 ggcccccatc cccggaaaac cgtatgttgg cgtggagatt cccaacccga aaagaaaggg
     1261 gattgctctt cgtaccatac ttgaatcgag agtttttctg gactcggaaa atgaccttcc
     1321 tcttgctatg ggcgttcgag tcgactcccg tccgttggtt gtggggctgg aggacttgcc
     1381 gcatcttctt gtcgccggaa ccacagggtc ggggaaaagc gtcttcgtta caagctgtat
     1441 aaccgggctc tgcacgcacc gctctccgga agagctcagg ctgatcctga ttgatccaaa
     1501 gcgggtagag ctgagcatct acgaacatct tcctcatgtg cttgccaaac cggtggtctc
     1561 tcccaaaaaa gccgtccagg cgcttgcatg ggctgtgcgg gagatggagc accggtatga
     1621 agttttcgcc cgggcaaggg tccgcaatct gaagtcgtac aaccagaatg ttctgcccaa
     1681 ggccaagctg cctcatatcg ttatcgtggt cgacgagctg gccgacctca tgttcaccgc
     1741 gcagaaagat gttgaagatt atatttgccg cctcgctcag atggccaggg cgactggtat
     1801 tcaccttgtt cttgctacac agcgtccgtc cgtcaatgta attacagggc ttatcaaagc
     1861 gaatatcccg gcgagagtcg catttactct tccttcgcag acggactcgc gtactattat
     1921 cgacgtctcg ggagcggagc gtctcctggg aaaaggcgat atgcttttcg tcagttcaaa
     1981 gtatccgcgc cctctgcgtc ttcaggctcc tttcatcgat gacggcaaga gcctcgagat
     2041 catcgagtac ctggggaata tctt
//
LOCUS       DAOSWW010000492         2063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44400_length_2063_cov_3.433765, whole genome shotgun sequence.
ACCESSION   DAOSWW010000492 DAOSWW010000000
VERSION     DAOSWW010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44400_length_2063_cov_3.433765"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PK442_09605"
     CDS             <1..592
                     /locus_tag="PK442_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO87949.1"
                     /translation="YSGKFSPTEAAAVAVLYAVVVEIFLYRHIGWRDLYRIALSTGIV
                     TAVVFILVAVRASFSWLVGYARIPEAILPPILGESPSMIKLLLVIAASYFIGCMFVDS
                     IVVIMILAPIFHPIATKLGIDPILIGVLVTMQAAIGAVTPPFGCNIFTAMAVFRRPYA
                     DVVRRIMPFLALYILATLIVIFFPSLSLFLRDMAFR"
     gene            772..1803
                     /gene="dctP"
                     /locus_tag="PK442_09610"
     CDS             772..1803
                     /gene="dctP"
                     /locus_tag="PK442_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO87950.1"
                     /translation="MKRFGKIAAIALVLVFVIGILSPAVAKEQWKFAIEEITGSVQDE
                     FAQFFKKKIMEKLDVDIEIYPVGVLGDSEDLTEQTMNGVLHFTMASPGHLGTFIPVVR
                     VFSLPFIWSDKMEVNKEVMKNSVAIYGLLEKEYEKHNLKLLGVFPEGWQVWTANKPLR
                     TPDDFKNFRMRIMGDPLLAEIYRAYGANAVQIPYPDLYSALQLKLADGNIQPYFAHEE
                     MKFYEQQDYFIDIREMPFVATFVVSLPWFQGLPDDQKKLIEEISKETIDFVFDMQEKM
                     NEERLQKMLKIKPSLQVIIPTAEEREVFKKLSLPVRDTYLKMAEPYGKDVLDTLVKEL
                     EEAEKKFAK"
     gene            1871..>2063
                     /locus_tag="PK442_09615"
     CDS             1871..>2063
                     /locus_tag="PK442_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOO87951.1"
                     /translation="MYFEVAEFHERLKKTKESMLRHKIEVLVVSDPANMNYLTGYDGW
                     SFYVHQGLVVALDRDEPLWW"
BASE COUNT          416 a          657 c          604 g          386 t
ORIGIN      
        1 ctactccgga aagttcagcc cgaccgaggc cgcggccgtc gccgtccttt acgccgtggt
       61 cgtggagatc ttcctctacc ggcacatcgg ctggagggac ctctaccgga tcgccctctc
      121 caccggcatc gtcaccgccg tcgtcttcat cctcgtggcc gtccgcgcct ccttctcgtg
      181 gctcgtcggc tacgcgcgca tcccggaggc catcctcccg ccgatcctgg gggagagccc
      241 ctccatgatc aagctgctgc tcgtcatcgc ggcctcctac ttcatcggct gcatgttcgt
      301 cgattccatc gtggtcatca tgatcctcgc gccaatcttc cacccgatcg cgacgaagct
      361 gggcatcgac ccgatcctca tcggcgtgct cgtcaccatg caggcggcca tcggcgcggt
      421 gacgccgccc ttcggctgca acatcttcac cgcgatggcg gtcttcagac gaccgtatgc
      481 ggatgtcgtc aggcgcatca tgcccttcct cgcgctctac atcctggcga cgctcatcgt
      541 catcttcttc ccctcgctct ccctcttcct gagggacatg gcgttccggt agcggcggga
      601 acgacgggac gaaagggggc gttcgcagga aacatgcaga ggaagggcgc ggcgaagcgg
      661 atttcttgac gccgccgggc gcgcatgatt tcgacgcctc aagaggcgtc gggttcagga
      721 aacgctgtac ggaaacaacg tgttctcaat ctgaaggagg tgcctgcagg tatgaaacgg
      781 ttcggaaaaa tcgcggcgat agcgctggtg ttggtcttcg tcatcggaat actctctccc
      841 gccgtggcga aggagcagtg gaagttcgcc atcgaggaga tcacgggaag cgtccaggac
      901 gagttcgcgc agttctttaa gaagaagatc atggagaagc tcgacgtcga tatcgaaatt
      961 taccccgtgg gcgtcctcgg agacagcgag gacctgacgg agcagaccat gaacggcgtg
     1021 ctccacttca ccatggcctc ccccggacac ctgggaacct tcatcccggt ggtccgcgtc
     1081 ttctccctcc ccttcatctg gtccgacaag atggaggtca acaaggaggt catgaagaac
     1141 agcgtcgcca tctacggcct tctcgagaag gagtacgaga agcacaacct gaagctcctc
     1201 ggcgtcttcc cggaggggtg gcaggtgtgg acggccaaca agccgctgcg cacgcccgac
     1261 gacttcaaga acttcaggat gcgcatcatg ggcgacccgc tcctcgcgga gatctacaga
     1321 gcctacggcg cgaacgcggt ccagatcccg taccccgatc tctactccgc gcttcagctg
     1381 aaactcgccg acggcaacat ccagccctac ttcgcccacg aggagatgaa gttctacgag
     1441 cagcaggact acttcatcga catccgcgag atgcccttcg tcgccacgtt cgtggtcagc
     1501 ctgccctggt tccaggggct tcccgacgac cagaagaagc tcatcgagga gatttcgaag
     1561 gagaccatcg acttcgtctt cgacatgcag gagaagatga acgaggagcg gctgcagaag
     1621 atgctcaaga tcaagccctc cctccaggtg atcatcccca ccgccgagga gcgcgaagtc
     1681 ttcaagaagc tctccctccc cgtgcgcgac acctacctca agatggccga gccttacggc
     1741 aaggacgtcc tggacactct cgtcaaggag cttgaggagg ccgagaagaa gttcgcgaag
     1801 tagaaagcag gcggggcggg ggctcgctct cccgcccctt cgtgaatgga cgaattggag
     1861 gcgaacgccg atgtactttg aagttgccga gtttcacgaa cgactgaaga agacgaaaga
     1921 gagcatgctg cggcacaaga tagaggtcct cgtggtgagc gaccccgcga acatgaacta
     1981 cctcaccggg tacgacggat ggtccttcta cgtccaccag gggctcgtgg tcgccctcga
     2041 ccgggacgag ccgctctggt ggg
//
LOCUS       DAOSWW010000493         2061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44483_length_2061_cov_3.702891, whole genome shotgun sequence.
ACCESSION   DAOSWW010000493 DAOSWW010000000
VERSION     DAOSWW010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44483_length_2061_cov_3.702891"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK442_09620"
     CDS             complement(<1..448)
                     /locus_tag="PK442_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO87952.1"
                     /translation="MDDSKSPSRLGRLFAARGMGQVVTAAFGLVILCVVFGILNPIFF
                     SGRNVGNLLRQIAPILLIGIGQSYVLITGNIDLSIGSVVGMSCMVSATLMTMGMNPWV
                     AVAVTLVCCLMVGVLNGCLVAKFNLPPFIATLGTMTIARGIAQIVNN"
     gene            complement(480..1997)
                     /locus_tag="PK442_09625"
     CDS             complement(480..1997)
                     /locus_tag="PK442_09625"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO87953.1"
                     /translation="MSEYIVELEGVGKSFAGIRALDDVSLHLKSGEVLALLGENGAGK
                     STLMKILGGIYTRDSGTIRVFGEEIDEMTPKRAKELGIAVIHQELNMCTHLTVAENIF
                     LGRELLRSGMLDDREMNIRAAEVLSRLNIDIAPDTVVGTLPISKQQMVEIAKALSTNA
                     RILVMDEPTSALTSKEINDLFHIIRSLRDQGCGIIYISHRLEELQHIVDRVTILRDGK
                     YIATTRFCDTTMAEIISNMVGREIKEKFPRVETPLGRTILRVKGLNAGRQVRNVSFEL
                     REGEIVGVAGLMGAGRTEMTRALFGADPKDGGEILLDERPLRIEKPMDAIEAGIVLVP
                     EDRKKDGLCTKLSIRENVALPNLDILKGALSTVNSRGEREMVERTVKNLSIRLSNAEQ
                     NADSLSGGNQQKLVVGKWLAREFRVVLFDEPTRGIDVAAKAEIYHLMNDLKTRGLGVL
                     FISSEMPEILGIADRIIVMCDGRVTGEFPAGTATQEKILECATRFEDKLGLAAGD"
BASE COUNT          376 a          681 c          577 g          427 t
ORIGIN      
        1 tgttgttcac gatctgcgcg atgccgcggg caatggtcat cgtccccagc gtcgcgatga
       61 agggggggag attgaacttc gccaccaggc atccgttgag gacgcctacc atcaggcagc
      121 ataccagcgt gacggccacg gcgacccagg gattcatccc catcgtcatc agcgtcgccg
      181 agaccatgca actcatcccc accaccgaac cgatagagag gtcgatattg ccggtgatga
      241 gcacatagga ctggccgatg ccgatcaaaa gaatcggcgc tatctggcgc agcagattcc
      301 cgacgttgcg cccggagaaa aagatcgggt tcagaatacc gaacaccacg cagaggatca
      361 ccagaccgaa cgcggcggtc acaacctgtc ccatgcctct cgccgcgaaa agacgcccga
      421 ggcgggaggg gcttttcgaa tcgtccatgc tcacagtcat cgctccccgt gtagaataat
      481 caatccccgg ccgcaaggcc gagcttgtcc tcgaagcgcg tggcgcattc gaggatcttc
      541 tcctgcgtcg cagtcccggc ggggaactcc cccgtcacgc ggccgtcgca cattacgata
      601 atcctgtccg cgataccgag gatctccggc atctccgagg agatgaagag cacgccgagc
      661 cctcttgttt tcaagtcgtt cataagatgg tatatctccg ccttcgccgc cacgtcgatc
      721 cccctggtcg gctcgtcgaa gagcacaacc cggaactccc gcgcgagcca cttgccgacc
      781 accagcttct gctggttgcc gccggagagg ctgtcggcgt tctgctccgc gttcgagagc
      841 ctgatcgaga ggttcttcac cgtccgctcc accatctccc gctcgcctct gctgttcacc
      901 gttgacaacg ctcccttgag gatatcgagg ttcggaaggg cgacattctc gcggatgctc
      961 agcttggtgc agagcccgtc cttttttctg tcctccggca cgaggacgat accggcctcg
     1021 atcgcgtcca tcggcttttc aattcgcaga ggccgttcgt cgagcaaaat ctccccgccg
     1081 tccttcggat ccgctccgaa gagcgcgcgg gtcatctcgg tcctgcctgc gcccatgagt
     1141 ccggcgacgc cgacgatctc tccttcgcgc agctcgaagc tcacgttccg cacctgacgc
     1201 cccgcgttca gccccttcac ccggagtatc gtccgcccga gcggtgtctc caccctcggg
     1261 aacttctctt tgatttcgcg tcccaccatg ttcgagatga tctccgccat ggtggtatcg
     1321 cagaacctgg tcgtcgcgat gtacttgccg tcgcggagga tggtcacgcg gtccacgata
     1381 tgctgcagct cttcaagccg gtgcgagata tagatgatgc cgcacccctg gtcgcgcagc
     1441 gaacggatga tatggaacag atcgttgatt tccttggaag tgagcgcgga ggtgggctcg
     1501 tccatgacga ggatccgcgc gttcgtggag agcgccttgg cgatctccac catctgctgc
     1561 ttcgagatgg gaagggttcc caccactgta tcgggagcaa tatcgatgtt cagccgggag
     1621 agcacctcgg cggcgcggat gttcatctcc ctgtcgtcga gcattcccga acggagcagc
     1681 tctctgccga ggaagatgtt ctccgcgacg gtgaggtggg tgcacatgtt cagctcctga
     1741 tggatgacgg cgatgcccag ctctttcgcc cgcttcggcg tcatctcgtc gatctcctcc
     1801 ccgaagactc tgatggttcc ggagtcgcgg gtatagatgc cgccgaggat cttcatcagc
     1861 gtcgattttc ccgcgccgtt ctcgcccagg agcgcgagca cctccccgct cttcaggtgc
     1921 aacgaaacgt cgtccagcgc cctgattccg gcgaaggatt tgccgacgcc ctcaagctcg
     1981 actatgtatt ccgacacacc gccacctccg ctcgcctcgc agatgcattc atgatcggcg
     2041 caggcgttct tttcctatac c
//
LOCUS       DAOSWW010000494         2059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44562_length_2059_cov_3.058383, whole genome shotgun sequence.
ACCESSION   DAOSWW010000494 DAOSWW010000000
VERSION     DAOSWW010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44562_length_2059_cov_3.058383"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /gene="guaA"
                     /locus_tag="PK442_09630"
     CDS             complement(<1..763)
                     /gene="guaA"
                     /locus_tag="PK442_09630"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOO87954.1"
                     /translation="MNNIVILDCGSQFTQLIARRIRELKVHSEILPWDAPIERIKSEA
                     LSGVVISGGPMSVSDEGAPTVPEELFGLGVPVLGVCYGMQLATKLFGGVVTSGSSREY
                     GRTAISVKEESALFSGTAASFEVWMSHGDHIDRLAPGFRCVAETANGVPAAMENPEKG
                     VWGLQFHPEVVHTAFGTEILSNFLFSVCGCVPDWDLGNWIDTMTEEIRRKVGPDRVIC
                     GLSGGVDSSVAAVLTARAVGDRLECIFVNNGLLRLN"
     gene            complement(901..1875)
                     /locus_tag="PK442_09635"
     CDS             complement(901..1875)
                     /locus_tag="PK442_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOO87955.1"
                     /translation="MKPFSLVPVFKDYLWGGTKLKTRFNKRTDVSPLAESWELAAHPD
                     GDCRIANGSFAGTPLSEFLRKFPESLGSGRACGGGLPVLIKLIDAREPLSVQVHPDDV
                     YAMRTEGGAGKTEMWYVLDREPGAFLYCGFQRDVSREELTRRIADGTVTEVLHKAPVE
                     PGDVFFMEAGTVHAIGAGILIAEIQQSSNTTYRVFDYGRRGPDGKERPLHIEKALDVA
                     RRGPACSVPPGSRPPVILPGSTLRRLARCAHFSVELLELSEHCEYRTDETSFLSLLCL
                     EGSARLAEGEWGFDIAKGDSVFVPARKGSVFLEGRGTFLLTTVPDGEL"
BASE COUNT          395 a          695 c          600 g          369 t
ORIGIN      
        1 cgttcaggcg caacagcccg ttgttgacga agatgcactc cagccggtcg ccgacggccc
       61 gcgccgtgag caccgccgcc acggacgagt cgaccccgcc ggagaggccg cagatcaccc
      121 tgtccggtcc gaccttccgg cggatttcct ccgtcatggt gtcgatccag ttgccgagat
      181 cccagtcggg cacgcatccg cagaccgaga agagaaagtt tgaaaggatc tccgtcccga
      241 aggccgtatg caccacctcg gggtggaact ggagtcccca gacgcccttc tccgggtttt
      301 ccatcgccgc cggaacgccg ttcgcggttt cggcgacgca gcggaatccg ggagcaagac
      361 ggtcgatgtg atcgccgtgg ctcatccaga cttcgaacga agcggctgtg ccggaaaaaa
      421 gcgccgactc ctccttgacg gagatcgccg tgcgtccgta ctcacgggaa ctgcccgacg
      481 tgacgacgcc gccgaagagc ttcgtcgcga gctgcatccc gtaacagacg ccaaggaccg
      541 gaacgccgag accgaacagc tcctcgggca ctgtcggagc gccctcgtcg gagacgctca
      601 tcggcccgcc ggagatgacg acacccgaca gggcctcgct cttgattcgc tcgatgggag
      661 cgtcccacgg cagaatctcg ctgtgtacct tcagctccct gattcttctc gcgatgagct
      721 gcgtgaactg ggagccgcaa tcgagtatga cgatgttgtt cacaagtcca ttccccctga
      781 aatatagaaa aaccggggcc ggaaagttcg gctttcacac cccgttgtat cttaccagaa
      841 agcccctcgc cgttccacaa gaggaaacga aaagcgccgc gaaaaccgcc ccgttccctc
      901 tcagagctct ccgtccggaa cagtcgtcag cagaaaagtt ccgcgtcctt ccaggaagac
      961 ggatcctttc cgcgcaggga cgaagacgct gtctcccttc gcgatatcga atccccactc
     1021 cccctcggcc aacctcgccg agccttcgag acagaggaga gagaggaagg atgtttcgtc
     1081 cgtccggtac tcgcagtgtt ccgaaagctc aagaagctcc acggagaagt gcgcgcatct
     1141 cgcgaggcgg cggagcgtgc ttccgggcaa gatgacggga ggccggctac ctggagggac
     1201 ggagcatgcg ggtcccctcc gggcgacgtc gagcgccttt tcgatgtgca gcggccgctc
     1261 ctttccgtcg ggaccgcggc ggccgtagtc gaagaccctg tacgtcgtgt tcgagctctg
     1321 ctggatctcg gcgatcagta tcccggcgcc gatggcgtgg accgttccag cctccatgaa
     1381 gaacacgtct cccggctcca cgggcgcttt gtgcagcacc tccgtcaccg tgccgtccgc
     1441 tatccgccgc gtcagctcct ccctggaaac atcccgttga aagccgcagt agagaaacgc
     1501 cccgggttcc cggtcgagta cgtaccacat ctccgtcttc cccgcgcccc cctccgtgcg
     1561 catcgcatac acgtcgtccg gatgcacctg taccgaaaga ggttccctgg cgtcgatcag
     1621 cttgatcagt acggggagcc cccctccgca ggcgcggccg cttcccaggg attcagggaa
     1681 cttccgcaga aactccgaca gcggggttcc ggcgaaggag ccgttcgcta tgcggcagtc
     1741 tccgtccgga tgcgcggcaa gctcccagct ctcggcgaga ggagatacgt cggtcctctt
     1801 gttgaaccgc gtcttgagtt tcgtcccgcc ccagagatag tccttgaaga cgggtacgag
     1861 tgaaaacggt ttcatgcatc ttcccccctt tcccctgtga aggaacgccg cccggcgttg
     1921 ctattcatcg aacagctccg actcgacgaa cgcacagagg aaatgataga tcctcaagtg
     1981 gtgctcctgg acgcggaacg tccttctgtc gggaacgcgg atgcagcagt ccgcatactt
     2041 cgccagcagc cccccgtcc
//
LOCUS       DAOSWW010000495         2050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44890_length_2050_cov_2.300251, whole genome shotgun sequence.
ACCESSION   DAOSWW010000495 DAOSWW010000000
VERSION     DAOSWW010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44890_length_2050_cov_2.300251"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..1439
                     /locus_tag="PK442_09640"
     CDS             321..1439
                     /locus_tag="PK442_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO87956.1"
                     /translation="MDDTEIETGVEGALFNYKGNRGFWAFITYCAELGQIVSTRYCDG
                     NVPPGWRQLEEFRNVLGTVPEGVKEVALRSDSAGYQAELLRFCNENPEKRFRRIYYGV
                     SADVNSEFRAAAKAVSETEWKPLKTYEDGNGKIVVLQEWAELAYAPNSLSTKKHGPDY
                     RFFAVREKWDGSGKDTEKEESPEKPVAGREGHGEQLYLEGAIETLEAKSDKVRKLHLT
                     QFGGSIYKLFGVASNIEDGADGEPFGLAAGEKMDGRAIIEWQRKRCGKSEEVHHIVKE
                     ELAGGHVPSKYFGANAAWFNAAALAFNLHTILKLFFLPEEYRKSRPRTLRFLLYTMAG
                     KIVNHGRRIVLKLWEGDYGGKLLAGALAKLESLPEPGS"
     gene            complement(1867..>2050)
                     /locus_tag="PK442_09645"
     CDS             complement(1867..>2050)
                     /locus_tag="PK442_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87957.1"
                     /translation="FGYRPDYPVENQMYFEGNKSDSPMDGAPQGDGRNKKSPDGAIRG
                     ARRAPTKLSSRDFPIV"
BASE COUNT          455 a          553 c          641 g          401 t
ORIGIN      
        1 agtccttctc tccctcgtgc ttctcaacct ggccggaggg acggccgtgg acaatctgaa
       61 ctttcttcgc gagaagctgg ccttcgagaa attcggcatc cgcattcctt ctccgagcgc
      121 aggcagagag tggctcaagt cttttcacaa cccctcggaa gactcgaaga gagggatggg
      181 acaagccttc atccccgagg agaacaagta tctgagcggt tggcgggacg tcttcgcgaa
      241 gtcgtttcat ttcgcgtgga agctgaaccc tcgcgccttt ctcacgttcg atatcgcgtc
      301 ctcgcaccga aggtgcgggg atggacgaca cggagataga gacgggagtg gagggtgctc
      361 tgttcaacta caaggggaac agaggattct gggccttcat aacctactgc gcggaacttg
      421 gtcagatcgt ctcgacgaga tactgcgacg ggaacgtgcc tccggggtgg aggcagcttg
      481 aggagttccg gaatgtgctc gggacggttc ccgagggagt gaaggaagtg gcgcttcgga
      541 gcgacagcgc cggataccag gcggaactgc tccgtttctg caacgagaat ccggagaagc
      601 gcttcaggcg gatctactac ggagtctccg cggacgtcaa cagcgagttc agagcggccg
      661 cgaaggccgt ttccgagacg gagtggaaac cgttgaagac gtacgaggac gggaacggga
      721 agatcgtcgt tctccaggag tgggcggagc tggcgtacgc gcccaacagt ctctcgacga
      781 agaagcacgg tccggactac cggttcttcg ccgtccggga gaagtgggac ggttccggga
      841 aggatacgga gaaggaggag tctccggaga agccggtcgc ggggcgggaa ggccatggcg
      901 agcagctcta cctcgagggg gccatcgaga ctctcgaagc gaagagcgac aaagtgagga
      961 agctgcacct cacccagttc ggcggaagca tctacaagtt gttcggagtg gcgagcaaca
     1021 tcgaggacgg agccgacggg gaacccttcg ggctcgccgc cggggagaag atggacggaa
     1081 gagcgatcat cgagtggcag cggaagcgct gcgggaagtc cgaggaagtc caccacatcg
     1141 tgaaggagga actcgcagga ggacacgtcc cgtcgaagta tttcggcgcg aacgccgcat
     1201 ggttcaacgc cgccgcgctc gccttcaatc tgcacaccat actgaagctc ttcttccttc
     1261 cggaagagta ccgcaagagc cgcccccgga cgctgcgctt cctgctctac accatggcgg
     1321 gtaagatcgt gaaccacgga aggcggatcg tgctgaaact ttgggagggg gactacggag
     1381 ggaaactcct ggccggagcg cttgcgaaac tggagtcgct gccggaaccg gggagctgat
     1441 tcacgtccgg gaaccgcctc atatttgtgg ataagtgcgg cgactccggc tccgcgctta
     1501 tgtttccgta tccacaattc gaaaagatac cgagtcgcgg agtgaaatat ctgaaaaagg
     1561 tcgcggaggg aaacaacttc tctcctccct ccctccgcga tgtcacaaat ttgccttgga
     1621 aaaacgaacc tgcggatttg ggtcgcggaa gggtgattga caagccccga ttttgctggt
     1681 agtctgaatt aattaaagat ctgcgcggcg cttcctttgg ggaattagac cccgtcaagg
     1741 ccagaccaag cggatgaaca agtgaaagcc ccgctcttgc acccccagtg aagaagaagc
     1801 tggcccggcc aagacgcggg gcttttatta tgcgcaggtt cagattgaca cgttcggctt
     1861 ctgctactac actattggaa aatctcttga tgagagcttc gttggggccc ttcttgcccc
     1921 tctgatcgct ccatcggggg attttttatt tctcccatcc ccctgcggtg ctccatccat
     1981 aggactatcc gatttattgc cttcaaagta catttgattt tccaccggat agtccgggcg
     2041 gtacccgaac
//
LOCUS       DAOSWW010000496         2049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44901_length_2049_cov_4.227683, whole genome shotgun sequence.
ACCESSION   DAOSWW010000496 DAOSWW010000000
VERSION     DAOSWW010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44901_length_2049_cov_4.227683"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1228)
                     /locus_tag="PK442_09650"
     CDS             complement(134..1228)
                     /locus_tag="PK442_09650"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO87958.1"
                     /translation="MRPVHIVYVTAGNGHRMAARALREAFDREDVPNVVMDLLDFSSD
                     LFKWSYSDVYAFVSEHSHLACRIMYDLTNKNREESIVLRMYEKISLENVKKFMRYIAE
                     NEPEKCICTHFFPLNVLSRMKAAGLYGGDLYTVITDYGLHKMWVNEYVDRYFIPSEMV
                     REGLLESGVPKEKIIMSGIPVLPKFARISEGKRSFSGRRLPVLFIASSIYNTLVVGIL
                     KELERTGLPLDVTVVTGRNHDLADDLERFSFNSKLSLNVLGFVDTLDKLMTKHDVLIT
                     KPGGLTVSEALCASIPMILVNPIPKQETNNSAYLQSKGAGILARTPEDVGGLVRRFYD
                     DRSTLSSMQRAAASIAFPNAASAVVSGVLR"
     gene            complement(1433..>2049)
                     /locus_tag="PK442_09655"
     CDS             complement(1433..>2049)
                     /locus_tag="PK442_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87959.1"
                     /translation="RLSEEMVEAVEKFCPLHFILNCVSDSSKHIVRVVGGHWYDAWRE
                     GIVTFRKYNFAPISRKADVVFLAAGGYPKDINMYQAHKALYMASRAVRPGGTLVFFAE
                     LAEGYGHKVFEEWARRGMTHDEVIAAFTADFRFGAHKLFYLAQLAKECTILLYSRAGE
                     EDSRLMFCEKCDLTDTLPFLVRKYGEDFTSYVIPQAGIVLPVEE"
BASE COUNT          465 a          586 c          564 g          434 t
ORIGIN      
        1 gtcgaaaaca gccttgataa tctggaaata gcggcgcgtc cgggcaggat cgtcgcagaa
       61 gaagaacatc ttctcccact ccagcttcat ttcttccgtt tttgcggcat cgtctcggga
      121 agccatcgtc cggtcacctc aacacgcccg aaacgaccgc ggaagcggcg ttcgggaagg
      181 cgatggaggc tgcggcgcgc tgcatcgaag agagtgttga acgatcgtcg tagaagcgac
      241 ggacaagccc gccgacatcc tcgggagttc gggcgaggat ccccgctcct ttcgattgaa
      301 ggtacgccga gttgttcgtc tcctgcttgg ggatggggtt cacaaggatc atcggtatgg
      361 aagcgcagag ggcttcgctc acggtgagcc cccccggttt ggtgatcagc acgtcgtgct
      421 tcgtcataag cttgtccagc gtatcgacga atcccaggac attcaatgat agcttggaat
      481 tgaatgaaaa gcgttcgaga tcgtccgcga gatcgtggtt ccgccccgtc accacggtga
      541 cgtcaagagg gagccccgtg cgctccagtt ctttaagtat accgacgaca agcgtgttgt
      601 aaatgctgct cgctatgaag agcacaggga gacgccttcc ggagaacgac cgcttcccct
      661 cgctgattcg tgcaaacttg gggagcaccg gtattcccga catgatgatc ttctccttgg
      721 gaaccccgga ctcaagaagc ccctcccgga ccatttcgct cgggataaaa tagcggtcca
      781 catactcgtt gacccacatc ttatgcaggc cgtagtcggt aataacggta taaagatccc
      841 cgccgtaaag cccggccgcc ttcatccggg agagaacatt caacggaaag aagtgggtgc
      901 agatgcactt ctccggttcg ttctccgcaa tgtaccgcat gaatttcttc acgttctcca
      961 gagaaatctt ttcgtacatg cggagaacga tactctcctc ccggttttta ttcgtgagat
     1021 cgtacatgat ccggcaggca agatgcgagt gctcgctgac aaaagcgtac acatcgctgt
     1081 acgaccactt gaagaggtcg ctcgaaaaat cgagaagatc catgacgacg ttcggcacgt
     1141 cctcgcggtc gaaagcctcc cgaagagcgc gggcagccat acgatggccg ttccccgccg
     1201 tgacatacac tatatgaacg ggacgcactg tttttccctc cgatgtctga gcattcccct
     1261 cgctgcgcac ttcattcagc agtggaaaat accctgtgga ttctctttat tgtaccgtat
     1321 gaaatgagaa ggaggaacag gaggaaaaaa gaaattccgg gttgaaaaga gagagggccg
     1381 ccaagccgta cacttgaagg ccctctctct cgtggaatta cgttgttgtg aactactcct
     1441 cgacaggcag cacaatgccg gcctggggaa tgacgtacga ggtaaagtcc tcgccgtact
     1501 tccgtacgag gaaaggaagc gtgtcggtca gatcgcactt ctcgcagaac atgagacggc
     1561 tgtcctcttc gccggctctg gagtacagaa ggattgtaca ctcctttgcc agctgggcga
     1621 ggtagaagag cttgtgcgcg ccgaagcgaa aatccgcagt gaacgcggcg atgacctcgt
     1681 catgcgtcat tccgcgccgt gcccactcct cgaacacctt atgcccgtac ccctcggcaa
     1741 gttcggcgaa gaagacgagc gtcccgccgg gacgaactgc tctcgacgcc atatagagcg
     1801 ccttgtgcgc ctggtacatg ttgatatcct tcggataccc tcccgcggcg agaaagacaa
     1861 cgtccgcttt cctggagatc ggggcgaagt tgtacttcct gaaagtgacg atcccctccc
     1921 gccacgcgtc gtaccagtgt cctccgacga cccggacgat gtgcttggag ctgtccgaga
     1981 cgcagttcag gatgaagtgc agcggacaga acttctccac cgcctccacc atctcttcgc
     2041 tgaggcgat
//
LOCUS       DAOSWW010000497         2048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_44935_length_2048_cov_3.528349, whole genome shotgun sequence.
ACCESSION   DAOSWW010000497 DAOSWW010000000
VERSION     DAOSWW010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44935_length_2048_cov_3.528349"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /gene="mnmE"
                     /locus_tag="PK442_09660"
     CDS             <1..1228
                     /gene="mnmE"
                     /locus_tag="PK442_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOO87960.1"
                     /translation="LLDEKGEALDQVLAVWFAAPKSYTGEDVVEIHTHGGTLVASKCL
                     ELLVCRGARLAEPGEFTKRAFLSGRIDLTQAEAVLGIIRSKSEEALRAATRTLRGELA
                     SFAMDIHQELLALSARMEVALDFPEEDIPLHDDDEILQSIYALCQSLEDLLDRCNTGF
                     LLREGIRVAIVGRPNVGKSSLLNALLKESRAIVTSIPGTTRDLIEEVLTYRGIPLRLV
                     DTAGMGGTPSDEVEALGIARAEATFEKADVRVWVVDGSSLPDEFDQSMSERIAGLEHV
                     VAVNKADLPRAKNAEEHTSEELLAEMLPGSTLLSISAKHGTGIDSLKDAIVSSIAGAG
                     TLDAGLNVSARQVEEIRAAIGSLKDASNALSGGLGQDAAASCLTESRLSMERLLGIAP
                     DDSLLDYIFSQFCVGK"
     gene            complement(1263..>2048)
                     /locus_tag="PK442_09665"
     CDS             complement(1263..>2048)
                     /locus_tag="PK442_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOO87961.1"
                     /translation="GSAHPPRLIHMVYRPSDSVRKVALVGKGVTFDSGGLNVKTGDSM
                     RTMKGDKTGASNVFAILRGAAKMNIPCELHGIVPVAENMPGGKSFRPDDILRIRNGKT
                     VEIENTDAEGRLLLADALCFACEQKPDAIIDMATLTGAWAVALGQNMAGLFTPDDAFA
                     DGFLEVSARRGERFWRLPMDDDRIAEAMKSPVADLVNCGSRYGGAIFAAQFLKEFISD
                     GIPWIHLDIAGVDMQKDEHSVYSKGATGFGVRSCLEYLLSISK"
BASE COUNT          452 a          611 c          540 g          445 t
ORIGIN      
        1 ccttctcgac gagaagggag aagcccttga ccaggtgctt gccgtctggt tcgccgctcc
       61 caagagttac accggagagg acgtcgtgga aatacatacg cacgggggaa cgctcgtcgc
      121 ctcgaagtgt cttgaactcc tcgtttgcag aggcgcacgg cttgctgaac ccggagaatt
      181 caccaaacgc gcgtttctct ccggacggat cgacttgact caggctgaag cggtactcgg
      241 catcatccgc tcgaaaagtg aggaagcgct tcgagccgcc accagaacgc tccgcggtga
      301 actcgcctca tttgctatgg atatccatca agaacttctc gctctctccg cccggatgga
      361 ggtagcgctc gattttcccg aggaggacat tcctctccat gatgacgatg agattctcca
      421 atccatttat gctctttgtc aaagtcttga agaccttctt gaccgctgca ataccggatt
      481 ccttctccgg gagggtatca gggtggctat tgtcgggcgg ccgaacgtag ggaaatcatc
      541 cctgctcaac gcgctcctta aagaatcccg cgcaatcgtc acatcaatcc ccggcacaac
      601 acgcgatctg attgaagaag tactcaccta tcgcgggatt ccgctccgac tcgtcgatac
      661 ggcgggtatg ggcgggacgc cctcggacga agtagaagcc ctggggattg cccgcgctga
      721 agctaccttc gaaaaagccg acgtcagggt gtgggttgtg gacggaagtt ccctccccga
      781 tgaattcgac cagtccatgt cggagcgtat tgcaggactt gaacatgtcg ttgcggtaaa
      841 caaagccgac ctaccgcggg cgaaaaatgc cgaagaacac acatcggagg agctgctcgc
      901 tgaaatgctg cccggaagta cgctcctttc aatctctgca aaacatggga cgggaatcga
      961 ttcgctgaag gacgctatcg tctcctccat cgccggcgcg ggtacgcttg acgccggatt
     1021 gaacgtctca gccaggcagg tagaggaaat tcgcgcagca ataggctctc taaaagatgc
     1081 ctcgaacgcg ctctccggag ggttagggca ggacgcggcg gcttcctgtc ttaccgaaag
     1141 ccgtctgtca atggaacgct tgttgggaat tgccccggac gactccctcc tcgactatat
     1201 tttcagccag ttctgcgtgg ggaaataaaa gagggggctt aagtccccct cttttccatt
     1261 tcttattttg aaatcgaaag aagatactcc agacagctcc ggacgccgaa acccgtcgcc
     1321 cccttggaat agactgaatg ctcgtccttt tgcatatcca cgcctgcaat atccaaatgt
     1381 atccaaggta ttccgtcgct gatgaattct ttcaggaact gcgccgcaaa aattgctccg
     1441 ccgtatctgc tgccgcagtt gacgagatcg gctaccggag acttcatcgc ttccgcaatc
     1501 cggtcgtcat ccatgggaag acgccagaat cgctctcccc tcctcgcaga aacctccaaa
     1561 aacccatcgg caaacgcgtc gtcaggagtg aaaaggcccg ccatgttctg tcccaaggct
     1621 accgcccacg ctccggtgag cgtcgccatg tcgatgatcg catcgggctt ctgttcgcag
     1681 gcaaaacaga gcgcgtcggc gagaagaagc cgtccctccg cgtccgtatt ctctatctcc
     1741 acagttttgc cgttccggat acgcagtata tcgtccgggc gaaacgattt gccgccgggc
     1801 atgttttcag cgaccgggac aatcccgtgc agttcgcagg ggatgttcat cttcgcggcg
     1861 cctcgaagaa tagcaaacac atttgacgct cccgttttgt cgcctttcat cgtccgcata
     1921 gaatctccgg tcttgacgtt cagtcctccg ctgtcgaacg tgacgccttt cccgacgagc
     1981 gcgacctttc gaacagaatc cgacggcctg tacaccatgt gaatcaaccg aggcggatgc
     2041 gccgaacc
//
LOCUS       DAOSWW010000498         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_45011_length_2046_cov_3.826720, whole genome shotgun sequence.
ACCESSION   DAOSWW010000498 DAOSWW010000000
VERSION     DAOSWW010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45011_length_2046_cov_3.826720"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK442_09670"
     CDS             <1..489
                     /locus_tag="PK442_09670"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOO87962.1"
                     /translation="VKKVSIVPIEVVVRNITTGSLCKRLGVEEGKVLPRPLVELYLKD
                     DALGDPLITEDHALLFGWSTEEQLLKIKEIALKVNSVLSEYFTGLRIILVDFKLEFGV
                     DAEGRLLLADEVSPDTCRFWDRDTKNRLDKDRFRKDLGDVLGAYQEIWRRISSSNEDG
                     AR"
     gene            486..737
                     /gene="purS"
                     /locus_tag="PK442_09675"
     CDS             486..737
                     /gene="purS"
                     /locus_tag="PK442_09675"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOO87963.1"
                     /translation="MTYRVSIFIELQEGVLDIQGKAVERSLAGMGHDALRSVKVGKYI
                     QVWVETGSAEDARKEAERMCDDLLVNSVIEQFRIEVEGE"
     gene            738..1442
                     /gene="purQ"
                     /locus_tag="PK442_09680"
     CDS             738..1442
                     /gene="purQ"
                     /locus_tag="PK442_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOO87964.1"
                     /translation="MNTAVIVFPGSNCDQDVMHAVAGSLGTKVRAVWHKETTLPEDTD
                     FVILPGGFSFGDYLRCGAMAARSPIMKAVAEHGRRGGLLLGICNGFQILTESRLLPGA
                     LLPNRTINFVCRPCFLRVESVSTPFTSLFSKGQVVQFPIAHGDGLYTLPEEELKALED
                     RGGVVFRYSDSDGLTGDNANPNGSANNIAGIVNERGNILGLMPHPERASEALTGGEDG
                     RVFWQSILESFTKGGR"
     gene            1444..>2046
                     /locus_tag="PK442_09685"
     CDS             1444..>2046
                     /locus_tag="PK442_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOO87965.1"
                     /translation="MEYTQAGLRETEYEEIRRVLGREPNECELRIMGVMWSEHCSYKS
                     TKNLLKLFPNNGDRVVLGPGENAGIIDLGDGLGAAFKAESHNHPSAVAPYQGAATGVG
                     GIIRDILALGARPVASMDGLFFGEPSSPRTQSLASGIVHGVGGYGNAVGVPTVGGKTA
                     YDPSYNENPLLNAFCIGLIPLDEIVSSRTARPGQSVVLLGS"
BASE COUNT          434 a          544 c          632 g          436 t
ORIGIN      
        1 gtaaaaaaag tatccatcgt tccgatcgaa gtcgttgtcc gcaacattac caccggctcg
       61 ctgtgcaagc gccttggagt cgaggaaggg aaagtcttgc cccgtcccct tgtggaactc
      121 tatctgaagg acgacgccct cggagatccg cttatcaccg aagatcatgc gctgcttttt
      181 ggatggagca ccgaggagca gcttttaaaa ataaaggaaa ttgctctgaa ggtcaatagc
      241 gttctttcgg agtacttcac gggattgcgt attatcctcg tcgatttcaa acttgagttc
      301 ggcgtcgatg ccgaaggccg cttgcttctg gcagacgaag tctcgcctga tacctgccgt
      361 ttctgggaca gggacacgaa gaaccgcctc gacaaagacc gtttccggaa ggacttggga
      421 gatgttcttg gagcgtatca ggaaatatgg agaagaattt cctcctcgaa tgaggatggg
      481 gcgcgatgac ctaccgggtt tccattttta tcgagctcca ggaaggtgtg cttgatatcc
      541 aggggaaagc ggttgaacgt tctcttgccg gtatggggca cgacgcgttg cgttcggtga
      601 aggtcggtaa gtacattcag gtgtgggtgg aaaccgggtc tgcggaggac gcacgaaaag
      661 aagccgagag aatgtgcgac gacctgctgg taaacagtgt catcgagcag ttccgtatcg
      721 aagtggaggg ggagtaagtg aacacggcgg tcatcgtctt tcccggcagc aactgcgacc
      781 aggatgtcat gcacgccgtc gccgggagcc tcgggacaaa ggtccgtgcc gtctggcaca
      841 aagagacgac gcttcccgaa gacaccgatt ttgttattct cccgggggga ttctccttcg
      901 gcgactacct tcgctgcggc gcgatggcgg ctcgttctcc tattatgaag gcggttgccg
      961 aacatgggcg ccgaggaggt ctgctgttgg gaatctgcaa cgggttccag attctgacag
     1021 agtcacggct gcttcccggc gcgctgcttc cgaaccgaac gatcaacttt gtctgccgcc
     1081 cctgtttctt gcgtgtggag agcgtatcga cgccgtttac ctcgctcttt tcgaaggggc
     1141 aggtcgttca gttccctatt gcccacggag acggactgta cacgctgcct gaagaggaac
     1201 tgaaggctct tgaagatcgc gggggcgttg tcttccgcta cagcgattcc gacgggctga
     1261 ccggcgacaa tgcgaatccc aacggatcgg cgaacaacat cgccgggatc gtcaatgagc
     1321 ggggcaacat ccttggtctt atgccgcacc ccgaaagggc gagcgaagcg cttaccggcg
     1381 gagaggacgg ccgggttttc tggcaatcta tcctcgaatc gtttacaaag ggaggccgat
     1441 gaaatggagt acacacaagc cggtcttcgc gagaccgaat acgaggagat ccgccgcgtc
     1501 ctgggcaggg agccgaatga atgcgagctc cgcatcatgg gggtcatgtg gtccgagcac
     1561 tgcagctaca agtcgacgaa gaacttgctg aagctctttc cgaacaacgg agatcgcgtg
     1621 gttctcggac cgggcgaaaa cgccggaatc atcgatcttg gagacgggct cggggcggcg
     1681 ttcaaggcgg agagtcacaa tcatccttca gcggttgccc cgtaccaggg agcagccacc
     1741 ggtgtgggag gaattatccg tgatattcta gcgctcggcg cacgaccggt cgcctcgatg
     1801 gacggactct tcttcggaga gccttcgtcc cccaggacgc agtcgcttgc atcgggtatc
     1861 gttcacggtg tcggagggta cgggaatgcc gtcggcgtgc ctactgtcgg cggaaagacg
     1921 gcatatgatc cgtcctataa cgaaaacccg ctgctgaacg cattctgcat cggactgatt
     1981 ccgctggacg aaatcgtcag ttcccgcacc gcgcgtccag ggcagtcggt cgtcctgctc
     2041 ggctcg
//
LOCUS       DAOSWW010000499         2036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_45372_length_2036_cov_3.497728, whole genome shotgun sequence.
ACCESSION   DAOSWW010000499 DAOSWW010000000
VERSION     DAOSWW010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45372_length_2036_cov_3.497728"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="PK442_09690"
     CDS             <1..1729
                     /locus_tag="PK442_09690"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOO87966.1"
                     /translation="REAPSVLARASAVVVTKSDQVEPERLERLVGELSRFVPKERLFF
                     SRISLLTWRRWNGGWKDVAGERPASVLAFSAIGSPESFRRSLEAGGVDILKEHRFKDH
                     YRYRMEDMAALEASLEECGASCMVCTEKDVYNLPQEWRPTRDILVPFISTVLDEEARF
                     RECLLEALRPRMVVASNGYGEDSMGVLLARKLKERFPSASVSAFPIVGRGEHYMKEGI
                     PIDSVPSDSPSGGVIKYRFADLWRDLRSGLLRSIARQMGAWKLLRGRIRTPLCVGDVY
                     LLLHALFGQGQLPVLIATAKTVYLSGHWRLERFLIKRRSRMAWTRDRDTAEELRRSGV
                     QARYDGNPIMDITCDNTIEPVSWGSENAPRILLLPGSRRRAYDDLVLLLQAVERIHAM
                     LSEGASYLMVVAPTLDTEKLLKACERVPATEEGQWTSFGGEHAPGVRKGTCEIRFFFG
                     PLPAVAGRAHLLVGLGGTANQVCAGMGVPVVSIEEKGKFVQKKLLGDSEVLVPPQPQA
                     LAETAVRILSDEPLRLRMAAEGMARLGGPGALDKVVEYAASRMGWDLRVRLYETLAGF
                     WGASDGRRP"
     gene            1774..>2036
                     /locus_tag="PK442_09695"
     CDS             1774..>2036
                     /locus_tag="PK442_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87967.1"
                     /translation="MNTSSCASERIPVPEGTGVCSEPVVRIADTSPEKLWDFSIGRGE
                     LTLIAGPCSLETPELGLEIAETVRDICASLGVRYIFKASFDKA"
BASE COUNT          362 a          591 c          677 g          406 t
ORIGIN      
        1 cagagaagcg ccgtcggtgc ttgcgagggc gtccgctgtc gtcgtcacca agtcggacca
       61 ggtggagccc gagcgcctgg agcgtcttgt cggggaactt tcccgcttcg tcccgaagga
      121 acggctgttc ttctcacgca tctcgctgct cacatggagg cgttggaacg gggggtggaa
      181 agacgtggcc ggagaacgac cggcctcggt gctcgcgttc tcggccatcg gcagtccgga
      241 gagctttcgc cgttctttgg aagccggagg cgtcgacatt ctgaaggaac accgctttaa
      301 agatcactac cgctaccgga tggaggacat ggccgcgctg gaggcttctt tggaggagtg
      361 cggcgcgtcg tgcatggtct gtaccgaaaa ggacgtctac aatctccctc aggagtggcg
      421 cccaacacgt gatattctgg ttccgttcat ctccaccgtg ctcgacgagg aggcgcgatt
      481 cagggagtgc cttctggaag ccctgcgccc ccggatggtg gtcgcgtcca acgggtacgg
      541 cgaggactcg atgggcgtgc tgctcgccag gaagctcaag gagcgttttc cgtccgcgtc
      601 ggtttcggcg ttccccatcg tcggaagggg cgagcactac atgaaggagg gaatcccgat
      661 cgactccgtt ccctccgact cgccgagtgg cggagtgatc aagtaccggt tcgccgacct
      721 ctggcgggat ctgcgctccg gccttcttcg ttccatagcc cgccagatgg gggcgtggaa
      781 gctgctccgc gggcgcatca ggaccccgtt gtgcgtcggc gacgtctacc tgttgctcca
      841 cgcgctcttc ggacagggac agcttcctgt tctcatcgcc acggcgaaga ctgtctacct
      901 cagcggtcac tggaggctcg aacgattcct catcaaacgc aggagccgga tggcgtggac
      961 ccgcgaccgg gatacggccg aggagctgcg gcgctcaggc gttcaggctc gctacgacgg
     1021 taatcccata atggacatta cgtgtgataa tactatcgag ccggtctcct ggggaagtga
     1081 gaacgcgccg cgtatcctgc ttcttcccgg gagcaggcga cgggcgtatg acgatcttgt
     1141 tctcctgttg caggccgtag agcgcataca cgctatgctt tcggaaggag cgtcctacct
     1201 catggtcgtc gcgcccactc tcgacacgga aaaactgctg aaagcctgcg agcgcgttcc
     1261 cgcgacggag gaaggacagt ggacttcttt cggcggagaa cacgcgccgg gagtacggaa
     1321 aggaacctgt gaaattcgct ttttcttcgg tccccttccg gcggtggccg gcagagcgca
     1381 ccttcttgtc gggctcggag ggaccgcaaa tcaggtatgc gcagggatgg gggttcccgt
     1441 ggtctccatc gaggagaagg gaaagtttgt ccagaagaag ctcctcggcg attccgaggt
     1501 gcttgtcccc ccgcagccgc aggcgcttgc ggaaactgcg gtgcgcattc tctccgacga
     1561 gccgctccgg ctgcgtatgg ccgccgaagg catggcgcgg ctgggggggc cgggagctct
     1621 ggacaaggtt gtggagtacg cggcctccag gatggggtgg gaccttcgcg tccgcctgta
     1681 tgaaacgctc gcggggttct ggggcgcgtc cgacggaaga cgtccttgac cgctgttcga
     1741 aatgattatt gagaatcagg agatgatgca gcagtgaata cttcctcatg cgcctcggag
     1801 cgcatccctg ttccggaggg aacgggtgtt tgctcggagc cggttgtccg catagcggat
     1861 acctcgcccg agaaactgtg ggatttttcg atagggagag gagaactcac actcatcgca
     1921 ggtccgtgct ccctggagac gccggagctg ggactcgaaa tcgccgagac ggtgcgggac
     1981 atctgcgcgt ccctcggggt gcgctatatc ttcaaggcct ccttcgacaa ggcgaa
//
LOCUS       DAOSWW010000500         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_45514_length_2032_cov_3.583713, whole genome shotgun sequence.
ACCESSION   DAOSWW010000500 DAOSWW010000000
VERSION     DAOSWW010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45514_length_2032_cov_3.583713"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1026)
                     /locus_tag="PK442_09700"
     CDS             complement(1..1026)
                     /locus_tag="PK442_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO87968.1"
                     /translation="MLLLLLYFAFSMFYVFSIKNSLFSQLPPLQMAAQGIIICIVSME
                     AGAHPILPWVGVLAAVSGFFILLHSYSQYGRGTHLPPPVAHAESPSSDTASEPWMRSP
                     FPYVVLDNEGTIIDANDAFASFAENGSVQGENVTTFFVPGETEAVVGGKKHAIFQKAK
                     DGQFHFLLVDNPPAQKNSKTAEAKSELELFDPKYGLYSRKYAQLRIPEELGRANRYRR
                     WLCGILLKVNYTYLPGRGRQPALEETFLAAYVLYVKESIRESDMGFSMQDGEFLLLLP
                     ETPQQGAKDTALKLIVVPESLDEMKKDYPFSVDIEYGFLYYSGNYPLSYDQFIQKLYA
                     SLGGGAE"
     gene            complement(1199..>2032)
                     /locus_tag="PK442_09705"
     CDS             complement(1199..>2032)
                     /locus_tag="PK442_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOO87969.1"
                     /translation="FARARQKTGLLVEGTVHFHTSMPSVSVAPPELRPLVADLAPKHK
                     DVRRFAMDEALPMMAPMVQAPAPAEPAPRPVIQTLTDLSTQAEGLLTGDYTPADFNLG
                     SFSLKSETSIISVPSLSEQAWLVAESKGLPFPILPASAELSVDGSPSGKTTLDEHGSG
                     SDILIAFGRTPRVKASKEKLVSKEGSTWTGRGRTEDGYTIEVTNGMPHPVAVTLKDRI
                     PISSRENISVEVLRIEPKPDEQSEQNLLTWKLSLAPGEKRTINAVFRLSYPSDETVIF
                     R"
BASE COUNT          512 a          505 c          542 g          473 t
ORIGIN      
        1 tcattcggcg ccccctccta aagaagcata aagcttttgg ataaactgat catacgaaag
       61 aggatagttc ccgctgtaat acaaaaatcc atactctata tcgaccgaga aaggatagtc
      121 cttcttcatc tcatcaagcg attcgggaac tactattagt ttgagtgccg tgtcttttgc
      181 gccttgctgt ggagtttcag ggagaaggag cagaaactcc ccgtcctgca tggaaaagcc
      241 catatcgctc tccctgatgg actccttgac gtagagaaca tacgcggcaa ggaaagtttc
      301 ctccagggca ggctgccggc ctcttccggg aaggtaggtg tagttcacct tgagtaaaat
      361 cccgcaaagc cagcgtctgt acctgtttgc tctgcccagt tcctccggga tacgcaactg
      421 ggcgtatttc ctggagtaga gtccgtattt cgggtcaaag agttcgagct cgctctttgc
      481 ttcagcggtc tttgaatttt tttgcgcagg agggttgtca acaagtaaaa aatggaactg
      541 accatccttc gccttctgaa aaattgcatg cttctttccc ccgacaacag cttctgtctc
      601 accgggaaca aaaaaggttg tcacattttc cccttgtaca gaaccgtttt cggcaaaaga
      661 cgcgaaggcg tcgtttgcat cgatgattgt cccctcgtta tccaaaacaa cgtatgggaa
      721 aggagacctc atccaaggtt cggatgctgt atcagaggaa ggggattcag cgtgcgcgac
      781 cggcggagga agatgtgttc cccttccgta ctgcgagtac gagtgcaaca atataaaaaa
      841 accagaaaca gcagcgagta cgcctaccca ggggagaatc ggatgagcgc ccgcctccat
      901 ggaaacgatg catatgatga ttccctgggc cgccatctga agcggtggta attgggaaaa
      961 caagctgttc tttattgaaa aaacatagaa catgctgaac gcaaaataca aaagaagtaa
     1021 aagcacccac aaaggggccc ccgccgatac tttgttgctc tcatttccga aagcgacgag
     1081 aaggagagag agaggcagcg aaagcagcgg aggtatggtg agccataaaa tcatggctgg
     1141 attctcctaa ttatatgttt gcttctcctg gacgccgagt accgcagaat gtagtatttc
     1201 aacgaaaaat caccgtctca tctgatgggt agctcaagcg gaaaaccgca tttatcgtac
     1261 gtttttcacc gggagcgaga gacagcttcc atgtcaggag attttgttct gactgttcgt
     1321 ccggcttggg ttcaatgcgc aatacttcga cagaaatatt ttcccgcgat gaaattggaa
     1381 tacggtcttt cagcgtgaca gcgacaggat ggggcatgcc gtttgtgact tcaatcgtat
     1441 acccgtcctc ggtgcgtcct cttcccgtcc atgtgcttcc ttcctttgag acgagtttct
     1501 ccttggaagc tttgactcga ggcgttctgc cgaaggcaat aagaatatcg gaaccggagc
     1561 cgtgctcatc aagcgttgtt ttcccggacg gacttccgtc gacggagagc tcggcggagg
     1621 ctggtagtat agggaaggga agccctttgg actctgctac gagccatgcc tgctcagaga
     1681 gagacgggac ggaaataata gaagtctctg acttcagcga aaagctgccc aggttaaaat
     1741 ctgccggagt ataatccccc gtcagcagtc cctcagcctg agttgaaagg tcggtaagcg
     1801 tctggataac ggggcgaggc gccggttctg cgggggcggg agcctgcacc attggggcca
     1861 tcatcgggag agcttcgtcc atggcgaaac gccgaacatc tttgtgtttc ggagccagat
     1921 ccgcgacgag cggacgcagt tccggaggcg ccacggaaac agaaggcatc gacgtatgga
     1981 agtgtacggt cccttcgaca agaagccccg tcttttgccg cgctcgcgca aa
//
LOCUS       DAOSWW010000501         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_45557_length_2031_cov_3.641194, whole genome shotgun sequence.
ACCESSION   DAOSWW010000501 DAOSWW010000000
VERSION     DAOSWW010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45557_length_2031_cov_3.641194"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1116
                     /locus_tag="PK442_09710"
     CDS             1..1116
                     /locus_tag="PK442_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOO87970.1"
                     /translation="MVTIVADELVKAAAACRDPFEFRDGDIVGVTESVLARSQGNYVT
                     LDDIAEDVRRRVPEGDVALIFPILSRNRFGRLLEGIARGVRGTLHVVLSYPADEVGNL
                     LIDPRAFYLKSGQLPGDLFGEKEYDEIFGRYPHPFTGVDYVSYYKDMDPKKIRIHFAN
                     NPLSALRFSKTVIVASIHTRFLHREILEKAGASVFSLDQFCSAPLAEGKGFNEQYGLL
                     GSNYSSETSVKLFPRESKRFARTLSEELLARTGKRVEVLVYGDGAFKDPAGGIWELAD
                     PVVSPGYTDGLEGLPKEVKFKYIADNAGDKSPDEAVREAIRNKGAMDRFAHSTLGTTP
                     RRLTDLLGSLCDLVSGSGDKGTPVVYISGYFDSYLDE"
     gene            1320..>2031
                     /locus_tag="PK442_09715"
     CDS             1320..>2031
                     /locus_tag="PK442_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO87971.1"
                     /translation="MRSMSTVRKMMALVFVLCMMAAPAFAADPIRIGEIATVTGDFAA
                     YGVAEVESVKIAVAEINAAGGILGRPVEVVMYDCRTRQEDMVNAARRLVEQDKVSVVI
                     GPSGSGLCIAAAPVFNRGQVPHIGTLPTNPLVTVDEKGAVRPYNFRICFLDPYQGKMI
                     AHFSAKDLGKKKAAILYDVSSDYSHGLREFFIASFKEFGGEIVADEGHRGEDIDFRAQ
                     LTVIKEANPDVLVVPTMGK"
BASE COUNT          395 a          596 c          634 g          406 t
ORIGIN      
        1 ctggttacaa tcgtcgccga cgagcttgtt aaggcggccg cggcgtgccg ggatccgttc
       61 gagttccgcg acggcgatat cgtcggcgtg acggagtcgg tgctcgcccg ttcgcagggg
      121 aactacgtca cgttggacga catcgccgag gatgttcgca gacgggttcc tgaaggggac
      181 gtcgcgctga tcttccccat tttgagccgc aaccgtttcg gacgcctgct cgaaggcatc
      241 gcgcgaggag ttcgcggcac gctgcacgtc gtcctctcct accctgcgga cgaggtgggc
      301 aatctgctca tcgacccgcg cgctttctac ctgaagagcg ggcagctccc cggcgacctt
      361 ttcggcgaga aggagtacga cgagatcttc ggccgctacc cccacccctt caccggcgtg
      421 gactacgtga gctactataa ggacatggac ccgaagaaga tacgcattca cttcgccaac
      481 aatccgctct ccgcgctgcg cttttcgaag acggtcatcg tggcgagtat ccacacccgc
      541 ttcctgcacc gggagattct ggagaaggcg ggagcttcgg ttttttcact cgaccagttc
      601 tgctccgcgc ctctcgccga gggcaaggga tttaacgagc agtacggtct cctggggtcc
      661 aactacagca gcgagacctc ggtgaagctt ttcccgaggg agagcaagcg ttttgcgcgc
      721 acgctttcgg aagaactctt ggcgagaacg gggaagcgcg tcgaggtgct cgtctacgga
      781 gacggcgcat tcaaagaccc cgccggaggc atctgggaac ttgccgaccc ggtggtgtcc
      841 cccggctata cggacggtct ggaggggctc ccgaaggagg tcaagttcaa atatattgcc
      901 gataacgcgg gggacaagtc tcctgacgaa gccgtccggg aggcgatccg gaacaagggc
      961 gcaatggacc gcttcgccca ctcgacgctg ggcacgaccc cgcgccgcct gaccgatctg
     1021 ctggggtcgc tctgcgacct tgtttcaggc tccggcgaca agggaacgcc tgtcgtgtac
     1081 atctcggggt acttcgattc ctatcttgac gagtgacggc gagcagcctg taaagagccg
     1141 aaaggctggt gcgaaaaacg cacagatgta gtattatgta ctttgtgagt acaaagaatt
     1201 tcggaccaat gtcgtctttc tctgcgggga tccggcggcc ggaaaaatcc gaccgtcttt
     1261 tctcaaatgc ggtactgttt cgtgcatcac gatcacatac tgtatgggag gtagaaagaa
     1321 tgaggagtat gagtacggtt cggaagatga tggcgctggt gtttgttctg tgcatgatgg
     1381 cggcccccgc ctttgcggcg gacccgatca ggatcggcga gatcgccacg gtgacgggcg
     1441 acttcgcggc ctacggcgtt gctgaagttg agtcggtgaa gatcgccgtc gcggagatca
     1501 acgccgccgg cggaatcctc ggcagaccgg tcgaggttgt tatgtacgac tgccgcacgc
     1561 ggcaggagga tatggtgaac gcggcccggc gtcttgtgga gcaggacaag gtcagcgtcg
     1621 tcatcggccc cagcggcagc ggcctgtgca tcgcggcggc tcccgtcttc aaccgcggac
     1681 aggttcccca tatcggaacc ctccccacga acccgcttgt caccgttgat gagaaaggcg
     1741 ctgtgcggcc ctacaatttc cgcatctgct tccttgaccc ctaccaggga aagatgatcg
     1801 cccacttctc ggcgaaggat ctcggcaaga agaaagcggc tattctctat gacgtgtcca
     1861 gcgactattc ccacggtctt cgcgagttct tcatcgcctc cttcaaggag ttcggcggag
     1921 agatcgtcgc ggacgaggga caccgcggag aggacattga cttccgcgcg cagcttaccg
     1981 tcatcaagga ggctaatccc gacgtcctgg tcgtcccgac gatgggcaag g
//
LOCUS       DAOSWW010000502         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_45584_length_2031_cov_2.019231, whole genome shotgun sequence.
ACCESSION   DAOSWW010000502 DAOSWW010000000
VERSION     DAOSWW010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45584_length_2031_cov_2.019231"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..713)
                     /locus_tag="PK442_09720"
     CDS             complement(237..713)
                     /locus_tag="PK442_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87972.1"
                     /translation="MNTRLFHRSNALSISVRVLLVLLFVLAVQTAGIAAETETEVEKE
                     KALIDWGQNYIEGSGMAVAPEGTTGAQAKALARRGAMVDLQRNMLEFVCGVQIDARTV
                     MDDFMAEDRVRSEVHGLIRNIEILKGEWDGESYTVTGRIKTEQLRLIVNYVLTLVR"
     gene            complement(808..>2031)
                     /locus_tag="PK442_09725"
     CDS             complement(808..>2031)
                     /locus_tag="PK442_09725"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOO87973.1"
                     /translation="LVDMSTAIEVGKIAGVQYILMGSVTELSEKMSGGAVPLFFIPVA
                     VGGGGHEAKATIDVRLIDTTTSETRLALSESGTSTNSASAVSVMGLSFAEAEFGGLQA
                     RAIADAVNRLAHGIRSELGGESSHVLNVSGNEVVIDVGSTMGAKEGQLYLVYAEGKTI
                     LGMSGESLGRNKMPLAVVKVRDVASGHSTCAVASNCSLSLVRRGDKIEPVTAAKAKTM
                     KFVSSRPAASSETFNQIFGPQGQSGAVASVPQDAPAPAAETPAKPAVHEDKKEEPAPA
                     AVSSVASAPRSSAPVAQAGEAKKIPGFDPNTSTDVKVIQTYNLDMGTSNMLRIAHVNA
                     MNSLNSRKVKVAYEKFVEAAGKYDGHYLAAYWAGVAAHRLKKADDSLQWMNRALAINP
                     QYQPAADYKSKKLKQ"
BASE COUNT          372 a          620 c          604 g          435 t
ORIGIN      
        1 cacgctcacc gccagactgg tcgacgtgga gacgggcgtc gtgcactcgg gattccagac
       61 agtcactcac cagggcgagg aggggctgat cgaggcctcc ggctttctcg ccgaggacat
      121 cgcgatggag ctgggcggcc cggacgtgtg aggagcgatt gatcatatcc ggaaaacgac
      181 atgcatttga gaagaagggc ggggaaagct gcaatcgcat tctccgccct tcttcgttac
      241 cgcaccagcg tcagcacata gttcacgatc aggcggagct gttccgtctt gatgcggccg
      301 gtgacggtgt agctttcgcc gtcccactcg cccttgagga tctcgatgtt gcggatcagg
      361 ccgtggactt cggagcggac gcggtcttcc gccatgaagt cgtccatgac ggtgcgggcg
      421 tctatctgga cgccgcagac gaactcgagc atgttacgct gcaagtcgac catcgcgcct
      481 ctgcgggcaa gggcctttgc ctgggcgccc gttgtgcctt ccggggcgac ggccatgccg
      541 gagccctcga tgtagttctg gccccagtcg atgagcgctt tttccttctc gacctccgtt
      601 tcggtttccg ccgctattcc ggccgtttga acggcaagga cgaacagaag cacgagcaga
      661 acgcgtacgg atatcgaaag agcattggag cgatgaaaga gacgggtatt cattgtcatg
      721 acctcctaag atgtcgatct tcccgcgttc aagcgcggga tacagcgccg cccccgcagg
      781 agcgagccga cgggggcgga tagtttgcta ctgtttcagt tttttgctct tgtagtcggc
      841 ggccggttgg tactgaggat tgatagcaag cgccctgttc atccactgga gggagtcgtc
      901 ggccttcttc agccggtgcg ccgccactcc cgcccagtac gccgcaagat aatgcccgtc
      961 gtacttgccc gccgcctcga cgaacttctc gtaagcaact tttacctttc tgctgttcag
     1021 agagttcatc gcgttgacat gggcgatgcg gagcatgttg gatgtaccca tatccaggtt
     1081 gtatgtctga atgactttca cgtcggtcga cgtgttggga tcgaaaccgg gaatcttctt
     1141 cgcctcgccc gcctgagcga caggtgcgga actccgggga gcggacgcaa cggaggatac
     1201 tgccgccggc gcgggctctt cttttttgtc ttcatgcacc gcaggctttg ctggtgtctc
     1261 cgcagcaggc gccggggcgt cctgaggaac ggacgctaca gctccgcttt ggccttgagg
     1321 gccgaaaatc tgattaaagg tctcgctcga ggccgcaggc ctgctggaga cgaacttcat
     1381 cgtcttcgcc ttcgccgcag tgaccggctc gatcttgtcg ccgcgccgca ccaggctcag
     1441 actgcagttg gaagcgacgg cgcaggtgct gtgtccgctc gcgacatccc gtactttcac
     1501 aacggcaagc ggcattttat ttctccccaa agactctccg ctcatcccga gaatcgtttt
     1561 tccttcggca taaaccagat acagctgtcc ttcctttgcg cccatcgtcg aaccgacatc
     1621 gatgacaacc tcgttcccgg acacgttgag cacgtgggaa ctctcgcctc cgagttccga
     1681 ccggattccg tgagccagac gattgaccgc gtccgctatc gcgcgcgcct gaagaccgcc
     1741 gaactccgct tcggcgaacg aaagccccat cacggagaca gcggaagccg agttcgtgga
     1801 tgtgccgctt tcggaaagag caaggcgggt ctcgctcgtc gtcgtgtcga taaggcggac
     1861 gtcgatggtc gccttcgcct catggccccc tccgcctacg gcaacgggga tgaagaaaag
     1921 cggaaccgcg ccccccgaca tcttctcgct gagttcagtc acggaaccca tcaggatgta
     1981 ctggactccc gcaatttttc cgacttcgat cgcggtgctc atgtcgacga g
//
LOCUS       DAOSWW010000503         2017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46111_length_2017_cov_2.827217, whole genome shotgun sequence.
ACCESSION   DAOSWW010000503 DAOSWW010000000
VERSION     DAOSWW010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46111_length_2017_cov_2.827217"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK442_09730"
     CDS             <1..388
                     /locus_tag="PK442_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase"
                     /protein_id="HOO87974.1"
                     /translation="HNSAYWTNGAVLALGAGAWGYADGIRYRNERTLIGYAEAIRRNG
                     HSVAEAETPDACKRAHEAAILMLRTARGISFEAFTSRYGEETLSSLLRTLEENAPPDC
                     FVRSDASFALSPKGMRVANALWSLIV"
     gene            409..1005
                     /locus_tag="PK442_09735"
     CDS             409..1005
                     /locus_tag="PK442_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87975.1"
                     /translation="MLPMEYGMIRFTPEDSYVVEDILIVLSRFFPARPVKGYSVEVAD
                     SLRGGLSFIEGDGDTDSLGKGLLLSLARVYVSMDEEGGSVLIAADSMEERARWISSQW
                     KSRSGKNFNGVIHHTYQGNGWSLVGIAAPGWMVLFDDETENAEQDWLEADLRVAYLPP
                     DDEVLDFLSPEKTSAEVLSLLAEYLLFCLPSREESPGI"
     gene            complement(1071..1739)
                     /gene="yedF"
                     /locus_tag="PK442_09740"
     CDS             complement(1071..1739)
                     /gene="yedF"
                     /locus_tag="PK442_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOO87976.1"
                     /translation="MGKIDARGRSCPQPVMMTKAHVEKGEDEIEVFLDNAISASNVQR
                     YLEKNGYFVRIFDEDGSIIIRASVITEASLPADNTPATQTADSAALHTPPLPKTVTVS
                     AAPAAARSTASTAVLITKKGIGGDDSLLGEVLMKGFLGTISQMAPLPSVLALMNEGVK
                     LAMKHTSSLDHLMLLEKKGVRILVCGTCTNHFGVTSDIGAGVISNMFEITEALLASDK
                     ILSL"
BASE COUNT          459 a          517 c          546 g          495 t
ORIGIN      
        1 gcacaacagc gcctactgga cgaacggcgc ggtgctcgct cttggcgcgg gagcgtgggg
       61 gtacgcggat ggtattcgct atcgcaacga acggacgctt atcggatatg ctgaagcaat
      121 ccggcgcaac ggacactcgg ttgccgaagc tgaaactccg gatgcttgta aacgtgcgca
      181 tgaagctgca atcctgatgc ttcggactgc cagaggtatc agcttcgaag cttttacctc
      241 gcggtacgga gaagaaacac tctcgtcact tcttcgtact ctggaggaga atgcgcctcc
      301 cgattgtttt gttcggagcg acgcatcttt tgcgctctcc ccgaagggga tgagggtcgc
      361 aaacgccctg tggagcctta ttgtgtaatc agcgttcagg aaaggaagat gctgccgatg
      421 gaatacggca tgatacgttt cactccggaa gattcttacg tcgtagagga tattctgatt
      481 gtgctctcgc gctttttccc cgcccgccct gtgaaggggt attccgttga ggtggctgat
      541 tcgctgcgcg gagggctttc ttttattgag ggggacgggg atacagactc ccttggcaag
      601 gggcttctcc tttcacttgc tcgcgtctat gtctcaatgg acgaagaggg ggggagcgtt
      661 ttgatcgccg ccgactcaat ggaagaacgg gctcgctgga tcagctcgca gtggaaatca
      721 cgctcaggga aaaatttcaa cggcgtcatt caccatacct atcaggggaa cggatggagc
      781 ctggtgggaa tcgcagcgcc gggatggatg gtgctctttg atgatgaaac tgaaaatgct
      841 gaacaggact ggttagaggc cgatcttcgc gttgcgtacc ttccccctga cgacgaggtg
      901 ctcgattttc tcagccccga aaaaacctct gctgaagttc tctcccttct tgccgaatat
      961 ctcctctttt gtcttccttc gagagaagaa tccccgggaa tataaccatc tttcaaaaaa
     1021 caataggcgc tcttcgataa cgacacgagg agcgcctatt gagcagtatc ctacaaagaa
     1081 agaatcttgt ccgaagcgag aagcgcttcg gtgatttcga acatattgga gatcaccccg
     1141 gcgccgatgt ccgatgtaac gccaaagtgg ttcgtacacg ttccgcagac aagaatgcgg
     1201 acgccttttt tctccagaag catcagatga tccaaagaag aagtatgctt catcgcaagc
     1261 tttacgcctt cgttcatcag ggcgagaacc gagggaagcg gagccatctg ggatatcgtt
     1321 ccaaggaacc ccttcataag aacctccccc aagagggaat cgtctcctcc tattcctttc
     1381 tttgtgatga gtacagctgt cgatgctgta gaacgagccg ccgccggtgc agcggagaca
     1441 gttacggtct tcggaagcgg aggagtatgc agcgcagcac tatctgccgt ttgcgttgct
     1501 ggcgtgttgt cggccggcag cgaggcttcg gtaatcacgg aagcgcgaat aatgatcgat
     1561 ccgtcctcat cgaaaatccg gacgaagtag ccgttttttt ctagatatcg ctgtacattg
     1621 gatgcggaaa tcgcattgtc gaggaaaact tcgatctcat cttccccttt ttctacatgc
     1681 gccttcgtca tcatgaccgg ttgcgggcag ctcctgcccc gtgcgtctat cttccccatt
     1741 ctactgcctc cctaccccgc gctttttaga aagcgcggag gaaataatga cacagtgttg
     1801 ctctccgtcg gaccaattat aatgtagaat agaaacaagg acaaattata tttgaaatag
     1861 ctgttcgtcc acactacggg acgatggaaa ggtgaatgat ctctcgtgaa tccgaacgaa
     1921 aaacaaacag gggcgtttcg ctcgcttcct tcaatggacg ttcttcttgg ttcgccgtgc
     1981 gtggaacctt tcttttcgat gatcgggcgc gagtccg
//
LOCUS       DAOSWW010000504         2014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46223_length_2014_cov_3.243492, whole genome shotgun sequence.
ACCESSION   DAOSWW010000504 DAOSWW010000000
VERSION     DAOSWW010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46223_length_2014_cov_3.243492"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1156
                     /locus_tag="PK442_09745"
     CDS             80..1156
                     /locus_tag="PK442_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87977.1"
                     /translation="MAELAVFAPEPPAAIGPEEPFEEEFSPKAPRGSEKPLDSRTAGG
                     SAGDKHKSGPLSGNAPEAKVRIVEGVPETIGSGKYGYCTINGETILLKDVCIDRIKYV
                     AVLGKEVGKPQCPLAFYEKLWSAVIPPEKEILGPRVDAFVPVVVDPEKASPAGDGSME
                     VYSPRYGRSIRLVFHGLREMPSEKEFTAFGTWKEPRSVLLVRSLLKPLAPLPPVSGKR
                     RRATRLLTGRWKEVYSSGERLRDFMETALDELSDPSGLSDETSEKLASLRSEYEENAS
                     LAMDRLHEDEKPEKRLENRGVVCLDMETLNNRDEAWEALCAADISSLPPAGYRERLKR
                     RGLHEKYTGSLDAVFASLLLNLEL"
     gene            1232..1642
                     /locus_tag="PK442_09750"
     CDS             1232..1642
                     /locus_tag="PK442_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87978.1"
                     /translation="MNKENTRTLFEAFPELYRGRFLDMKENLMCFGFECDDGWFELLY
                     DLSREITEHCAASGDPVPLAVQVKEKFGTLRFYADNTSKAVDDIIEKYQEKSGGICEI
                     TGAPGKLCHRRGRLKTLSEEEARKDGFCPGTKTT"
     gene            1733..>2014
                     /locus_tag="PK442_09755"
     CDS             1733..>2014
                     /locus_tag="PK442_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87979.1"
                     /translation="MAQRKNRHTEAVGMERADELCTGKVCVLGEKRSVAAIMNALLEE
                     CSVDVPTVVLCHDPAIAERSARKEDAVVVMGDRPEAGERFPELLEPWLHS"
BASE COUNT          498 a          514 c          603 g          399 t
ORIGIN      
        1 ccccttcgac gaacgggaaa cagtctaact ggagggggaa ataacctcgc tttcaaagga
       61 agatattttt ctggcgaaca tggccgaact tgctgtattt gctccggaac cccctgcggc
      121 aatcggaccg gaagagccgt ttgaagaaga gttttcgcca aaagcgcccc gggggagcga
      181 gaaaccgctc gactccagga cggcgggagg aagcgccggg gataaacata agagcggtcc
      241 tctttccggt aatgcaccgg aggcgaaggt ccgcatcgtc gaaggagtac cggagacgat
      301 aggttccgga aaatacggct actgcacaat aaatggagag actatcctac tgaaggacgt
      361 ttgtatcgac cggatcaagt acgttgccgt tctgggaaag gaggtcggaa agccccagtg
      421 cccgctcgcg ttttacgaaa aactctggtc cgccgttatc ccgccggaaa aagagatctt
      481 gggtccaaga gtcgacgcct tcgttcccgt cgtcgtggat ccggaaaaag cgagtccggc
      541 tggagacggg tccatggagg tgtattctcc ccgctacggg cggtccatcc gccttgtgtt
      601 tcatggactg agggagatgc cttccgaaaa agagttcacc gccttcggca cgtggaaaga
      661 accgcggagc gttcttcttg tccgttcgtt gctgaagcct cttgcgccgc ttccgccggt
      721 ctcagggaaa aggcgccggg ctacccgact gcttacggga cggtggaagg aagtgtactc
      781 aagcggagaa cggctgcgcg acttcatgga gacggctctc gacgaacttt cggatccctc
      841 cgggctgtct gatgaaacca gcgaaaagct ggcctctctg cgttcggagt acgaggaaaa
      901 cgcctcactt gcgatggatc gtttgcacga agatgaaaag cctgagaaac gcctcgaaaa
      961 cagaggagtc gtctgtctgg acatggaaac tttgaacaac cgggatgaag cgtgggaagc
     1021 gctctgcgcg gcggacatct cctcacttcc tcccgccggc tacagggaac gcctgaaaag
     1081 gcgcggactc catgaaaagt acaccgggag tctggacgct gtttttgcct cgctgctgct
     1141 caatcttgag ctctaaacca tcgaaaatag cgataaagcc gttggaaacg ggcgaagcaa
     1201 ccgcctatga tgaaaaacag gaggcgccgc tatgaataaa gaaaatacgc gcacactctt
     1261 cgaagctttt ccggaactct accgtggaag gtttctcgat atgaaggaaa acttgatgtg
     1321 cttcggcttc gaatgcgacg acggctggtt tgaacttctg tacgaccttt cgcgtgaaat
     1381 taccgaacac tgtgcggcat ccggcgatcc cgtgccgttg gccgtacagg tgaaagagaa
     1441 attcggaacg ctccgctttt atgccgacaa cacgtccaaa gcagttgacg acattatcga
     1501 gaagtatcag gagaagtccg gcggaatttg cgagataacg ggagcgccgg ggaagctctg
     1561 tcacaggagg gggcggctga agactctctc cgaagaagaa gccaggaagg acggcttttg
     1621 cccgggaacg aaaaccacgt agatttcttc agcgtctcaa acgcggcttg acaagtagat
     1681 atctttaaga tagtatataa gtatctaaaa gaccgcaagg gagatgtgtt ctatggcgca
     1741 acgaaaaaat cggcatactg aagcagtcgg gatggaacgg gcggatgagc tgtgtaccgg
     1801 aaaagtctgc gttctgggag agaaacgaag cgtcgccgcg atcatgaacg ctctgctcga
     1861 agagtgctcc gtggatgtcc cgactgtcgt tctgtgccat gatcccgcca tagcggagcg
     1921 gtccgcccgg aaagaggatg ccgtcgttgt catgggcgac aggcccgagg ccggagagcg
     1981 gttccccgag ctcttggagc cctggcttca cagc
//
LOCUS       DAOSWW010000505         2014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46228_length_2014_cov_3.090352, whole genome shotgun sequence.
ACCESSION   DAOSWW010000505 DAOSWW010000000
VERSION     DAOSWW010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46228_length_2014_cov_3.090352"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="PK442_09760"
     CDS             complement(<1..1426)
                     /locus_tag="PK442_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HOO87980.1"
                     /translation="MKRRNGTLCFSAGDLCVFTESPFAAWMDRWYEETAGRAPSTLLF
                     AGESFEQCAPDAPDAEMRLLAGKGAEHERAFLEKLRNEGRSVADLAGERDPEATRRAL
                     LSGVDVLYQPYLEAPPFFGYADFLLRCGDGPRYEVCDAKLARSIKPVFPLQLCLYSEM
                     LAPLQGEIPERFHIALGSGETRSFRIERFLHLYRALRERFLAFQETFDPSAPPHPGDS
                     PAHGGWSGTAGRILERLDSLARVANIAGSQRKKLEKAGIVRMKDLAETREERVPGLSP
                     DVFARLREQASLQIASEGKERPEYRIVPSADGEELLPPASPGDVYFDIEGYPHAEGGL
                     EYLWGAVGTDTPAPSGEGEVVFYDWWAHDAAGEKAAFEQFIDWVRARRTLHPGMHVYH
                     YAPYETTAAKRLAAKYATREEEVDILLREGVFVDLYRVVRRALLVGTPGYSLKDVEHL
                     YREKRSGDVATGGASVVAYHEWTVS"
     gene            complement(1517..>2014)
                     /locus_tag="PK442_09765"
     CDS             complement(1517..>2014)
                     /locus_tag="PK442_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87981.1"
                     /translation="VHDTLEERQEKIASYDDPFVAVLLAAADFEWTVRRAILALGISA
                     TKEIKEDVLSRCSGLDKYKDAWKKEVEPLTGKGLSEIIPDWQYVKEKAYPLRNRLVHG
                     IDGVVTSQYAQERVKAILSASKAVAEFADSFGEPLYGRRIVRLKRRAKLRGSSSAKKK
                     QESRS"
BASE COUNT          371 a          629 c          629 g          385 t
ORIGIN      
        1 cgcttacagt ccactcgtgg taggcgacca cggaagcgcc gcccgtcgcc acatctccgg
       61 agcgcttctc ccggtagagg tgctccacat ccttcaggga gtagcccgga gttcccacca
      121 gcagcgctct ccggacgacg cggtagagat cgacgaacac tccttcgcgg aggaggatgt
      181 cgacttcctc ctcccgggtg gcgtacttcg cggcaagtcg cttggccgcc gtggtctcgt
      241 acggggcgta gtggtagacg tgcattccgg ggtggagcgt cctgcgcgcg cggacccagt
      301 cgatgaactg ttcgaaggcg gccttctctc cggcggcgtc gtgcgcccac cagtcgtaga
      361 acacgacctc accctccccc gaaggggccg gcgtgtccgt gccgacggcg ccccagaggt
      421 attcgagtcc gccttcggcg tgcgggtagc cttcgatgtc gaaatagacg tcgccggggg
      481 aggcgggagg aagaagctcc tccccgtcgg cgctcggaac aatgcgatac tccggccgct
      541 cctttccttc ggaggcgatc tgcaaggaag cctgctcgcg gaggcgggcg aagacgtcgg
      601 gggagagacc ggggacgcgc tcttcccgag tttcggccag atccttcatt cgcacgattc
      661 cggccttctc cagcttcttc cgctgcgatc cggcgatgtt ggcgacgcgg gcgagcgagt
      721 cgagccgctc gagaatgcgc cccgccgtcc cgctccatcc gccgtgcgcc ggggagtctc
      781 ccggatgcgg cggcgccgac ggatcgaacg tctcctgaaa tgccagaaag cgctcgcgaa
      841 gcgcgcggta gaggtggaga aagcgctcga tccggaagct tctcgtctcg ccggaaccga
      901 gggcgatatg aaacctttcg ggaatctccc cctggagggg agcgagcatc tcggagtaga
      961 ggcagagctg aagggggaag accggtttga tcgaacgggc cagcttggcg tcgcagactt
     1021 cgtagcgcgg tccgtcgccg caacggagca ggaagtccgc gtatccgaag aagggaggcg
     1081 cttcgaggta cggctggtag agcacgtcga cgccggagag cagggctcgc cgggtcgcct
     1141 cgggatcgcg ctcccccgcg agatcggcga cgcttctccc ttcgttccgc agcttttcga
     1201 ggaaggcgcg ttcgtgctcg gcgcccttgc ccgccagaag acgcatctcg gcgtccggtg
     1261 catccggagc gcactgctcg aaagactctc cggcgaaaag aagcgtcgag ggagcgcgtc
     1321 cggccgtctc ttcgtaccag cggtccatcc atgcggcgaa ggggctttcg gtgaaaacgc
     1381 acaaatctcc tgcggaaaag cacagcgttc cgttgcggcg tttcaagagc atcatctcct
     1441 cggcttgatt cgggatccag gaagtgcgcc ggcaaagcgt ccgtttgcag ggaacggcgc
     1501 agagaaccgt tgtcgttcag gagcggctct cttgcttctt tttcgcagaa gaggaacccc
     1561 gcaacttcgc gcggcgtttt aatctgacaa ttctgcggcc ataaagaggt tcaccgaagg
     1621 aatcggcgaa ctccgcgacc gccttgcttg ccgaaaggat cgcctttacc ctttcctgcg
     1681 catattgaga agtaacgacg ccatcgatcc catggacgag gcggttccgc agcggatagg
     1741 ccttttcttt gacatactgc cagtccggta ttatttccga aagccctttc ccggtcaacg
     1801 gttcaacttc cttcttccaa gcatctttgt atttatcaag accggaacac cgactcagca
     1861 catcctcctt gatctctttc gtagcgctga tgcccaaggc aaggatagca cgcctgacgg
     1921 tccattcgaa atccgctgcc gccaagagga cggcaacaaa gggatcatcg taagaagcga
     1981 ttttctcctg cctttcttcg agcgtatcgt ggac
//
LOCUS       DAOSWW010000506         2009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46423_length_2009_cov_3.425793, whole genome shotgun sequence.
ACCESSION   DAOSWW010000506 DAOSWW010000000
VERSION     DAOSWW010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46423_length_2009_cov_3.425793"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PK442_09770"
     CDS             complement(<1..299)
                     /locus_tag="PK442_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87982.1"
                     /translation="MKRAAPSKGALRAPLWIAVYVAAMGAFESLVPEAAVTLWRAPLV
                     AALSLVLILALRKRDAFAFYGFVWPKPAALRGALYGLPLVALATANLWHGATLNLG"
     gene            complement(372..1460)
                     /locus_tag="PK442_09775"
     CDS             complement(372..1460)
                     /locus_tag="PK442_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HOO87983.1"
                     /translation="MKKQIFRQSALDRLSSPEQLDMLFQVVRPAYWAALSALLLLLTV
                     LVVWGFYGTVTTMVSGQGILLREGARVDASSPGSGRLEVIFVDIDDPVEKGHVIARLA
                     QPDLRNQIAEMRGRVEILRAERAMGTEMGVDKKTLESEFLARRRQALNSSIKVARDRA
                     ESLREQLEQYDALYAKKYITRSQYLDVKDRYNAALQEILSFNEELARLPMTAADTTSQ
                     IERQRIDVDMRLLTAEKQLDALEERYDLETSVRSPETGRVLEIFKLEGQVISAGEALL
                     SLEKSDDSGAPLYVHAYVQPQDGKKILPEMEAQISPSVVKQEEFGVLRGRVTQVSSFP
                     ASARGMTRVLGNEQLVQALSRGGAPIAG"
     gene            complement(1746..>2009)
                     /locus_tag="PK442_09780"
     CDS             complement(1746..>2009)
                     /locus_tag="PK442_09780"
                     /inference="COORDINATES: protein motif:HMM:NF021923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HOO87984.1"
                     /translation="VGLQEKLTELGTKENVSQYVRDELGYDFTLEEMQKVIFERHPEM
                     TDEELEAVVGGTTASEIGKAAATGTAIGLGLCALASLLAAAAA"
BASE COUNT          396 a          661 c          566 g          386 t
ORIGIN      
        1 cccaagttga gagtagcgcc atgccatagg ttagccgtcg ccagcgcgac gagcggtagc
       61 ccgtagagcg cgccccgtag agcggcgggc ttcggccata caaaaccgta gaacgcgaac
      121 gcgtcgcgct tccgtaaagc caggatcagg acgagcgaca gcgcggccac caacggcgcc
      181 ctccagagcg tcacggcggc ctcgggtacg agggactcga acgcgcccat ggccgcgacg
      241 tagacggcga tccagagggg cgcgcgaaga gcgcctttcg atggagcggc tcgtttcatt
      301 cttcttcctc aattacgatg attaacgggg gatatatata ccgttttctg ctgtgatcaa
      361 caagcttcgt actacccggc tatcggagcg ccgccgcgtg aaagcgcctg gacgagctgc
      421 tcgttcccca gcacgcgcgt catccccctc gcggacgcgg ggaatgagga gacctgcgtc
      481 acccgtccgc gaaggacgcc gaactcctcc tgcttcacca cggacgggga gatctgcgcc
      541 tccatctccg gcaggatctt cttgccgtcc tgcggctgga cgtaggcgtg cacgtacagc
      601 ggcgcgccgc tgtcgtcgct cttttcaagc gagagcagcg cctcccccgc ggagatgacc
      661 tgtccctcca gcttgaaaat ttccagcacg cgcccggtct cggggctgcg aacggaagtc
      721 tccagatcgt agcgctcctc gagcgcgtca agctgtttct ccgccgtcag caggcgcatg
      781 tccacgtcga tccgctgccg ctctatctgc gaagtagtgt cggcggcggt catcggcagc
      841 cgggccagct cctcgttgaa ggagagaatc tcctgaagcg cggcgttgta ccggtccttc
      901 acatccaggt actggctccg cgtgatgtac ttcttcgcat agagggcgtc gtactgctcg
      961 agctgctcgc ggagcgactc ggcgcggtcg cgcgccacct tgatcgaaga gttcagcgcc
     1021 tgtctgcgcc gcgcgaggaa ctccgactcc agcgtttttt tatcgacgcc catctccgtc
     1081 cccatcgccc gctcggcacg caaaatctcc accctgcccc gcatctcggc gatctggttc
     1141 cgcagatcgg gctgggcaag ccgggcaatc acatgcccct tctcgacggg atcgtcgatg
     1201 tcgacgaaaa taacctccag gcggccgctt cccggagacg aggcgtcgac gcgcgcgcct
     1261 tcccgcagca gaataccctg cccggagacc atcgtcgtca ccgtgccgta gaacccccag
     1321 acaaccagga cggtcaacag gagcagcagt gcggagagcg ccgcccagta cgcgggccgg
     1381 acgacctgaa agagcatgtc gagctgttcg ggggaggaaa gcctgtcgag agccgactgg
     1441 cggaaaatct gtttcttcac ggtcgggcac gctccttcaa ggaaaacatg taccggcttt
     1501 ttcccggtat gcgcacgaga actcctgtta tgcattgaat tataccccga cacttccggg
     1561 agaaacagag atgtctgcgc atttttcccc cgtcggactg tcctgttata cggagctggg
     1621 gcataaacaa aaaaggaggg gagaatttcc cctcctcaaa aaaacgatcc cgtatattcg
     1681 atttcaaaaa gacgctcggt taacacagag gacacctccg aaaccgggaa tcccggcaaa
     1741 ttccgctatg ccgctgctgc tgccaataat gatgccagcg cacagagtcc caaacctata
     1801 gcggtacccg ttgccgcagc cttgcctatt tctgatgccg ttgttccgcc gacaaccgct
     1861 tccagttcct cgtcggtcat ctccggatgg cgctcgaaga tcaccttctg catctcttcc
     1921 agggtgaagt cgtaccccag ttcatccctc acgtactgtg agacattctc cttcgtgcca
     1981 agctccgtca gcttctcctg caacccgac
//
LOCUS       DAOSWW010000507         2007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46502_length_2007_cov_3.217725, whole genome shotgun sequence.
ACCESSION   DAOSWW010000507 DAOSWW010000000
VERSION     DAOSWW010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46502_length_2007_cov_3.217725"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PK442_09785"
     CDS             <1..694
                     /locus_tag="PK442_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87985.1"
                     /translation="DLFRFNRNAGAVLAGAAACFIFTNPLLNVVGYIGAVSVGQFNYV
                     EWMLEKNMLLALVGVLAWTTSLWTTNNAELYCNALYTGPVFWSYGRLIERKPLVLWAG
                     VLGTLLGSLAFYQIFFADFITVLGAAAPPLAGPLIADYYFVKKRRYIAENLENEPAYR
                     MTGVLSFLAGGSLGLFFQYGAPLPYGLPSGLFALLATIVLYVALYKATPDAAYDATLF
                     AASERKAQEVRS"
     gene            691..1464
                     /locus_tag="PK442_09790"
     CDS             691..1464
                     /locus_tag="PK442_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOO87986.1"
                     /translation="MRKVFLNEFSSSELERMISAGEIDSAITIFGSCESHGPHLPLGP
                     DLFVPTEIARRAALRLARTVVVPGVPFGTSLHYNRFPLSVTLRFETTIAVAEDIFTSL
                     IENGIRRIVILNGHDGNIPALEIAARKVKDRFPDATLVYLPAWWNITGAKMADDFEVW
                     NGLGHGGEGETSIMMAARPDLVDLTQAKSQVPHDVIALSDFATVIWDIAEITSTGATG
                     DSTKATQEKGEKMLRCVTDYLVGLVEELERRGWKYDLKK"
     gene            1582..>2007
                     /locus_tag="PK442_09795"
     CDS             1582..>2007
                     /locus_tag="PK442_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO87987.1"
                     /translation="MNISSIIEKANALTEKITELRRMFHAHPELPWLETETSLFVEQY
                     LRKLGLENIRRGFDGTQSGVVADLKGGKPGRCVALRADIDALPLEEENDVPYKSTHSG
                     VMHACGHDAHTAVLLGVAEILSSMRDELCGTVRFLFQPAE"
BASE COUNT          394 a          564 c          644 g          405 t
ORIGIN      
        1 ggatctcttc cgcttcaaca ggaacgcggg agcggtcctc gcaggcgccg cggcctgctt
       61 catcttcacg aaccccctgc tcaacgtcgt cgggtatatc ggcgcggtgt cggtggggca
      121 gttcaactat gtcgagtgga tgctggagaa gaatatgctg ctggcgctgg tcggcgttct
      181 cgcgtggacg acctcgttgt ggaccacgaa caacgcggag ctgtactgca acgcgctcta
      241 cacggggccg gtcttctggt cgtacggcag gctgatcgaa agaaaaccgc tcgtgctgtg
      301 ggcgggggtc ttggggacgc tgctcggttc gctggcattc taccagatct tcttcgccga
      361 cttcatcacc gtgctggggg cggccgcccc tccgctggcc gggccgctca tcgcggacta
      421 ctactttgtc aaaaaaaggc ggtatatcgc ggagaacctg gagaacgagc ccgcgtacag
      481 aatgacggga gtgctctcct tccttgccgg ggggagcctg gggctcttct tccagtacgg
      541 cgcgccgcta ccgtacggcc tcccgtcggg gcttttcgcg cttctggcga cgatcgttct
      601 ttatgttgcg ctctataaag caacaccgga cgccgcctac gacgcgacgc tcttcgcggc
      661 atcggagaga aaagcgcagg aggtgaggtc atgagaaaag tgtttttgaa cgagttttcc
      721 tcttccgagc ttgaacggat gatcagcgcc ggggagatcg acagcgcaat tacaattttc
      781 ggcagctgcg agagccacgg tccgcatctg ccgctcggtc cggatctctt cgtcccgacg
      841 gagattgcgc gacgcgcggc gcttcggctg gcgcgcaccg ttgtcgttcc cggagtaccg
      901 ttcgggacgt cgttgcacta taaccggttt cccctgtcgg tgacgctccg tttcgaaacg
      961 acgattgcgg tggcggagga catttttacg agcctgatcg aaaacggcat ccgccggatc
     1021 gtcatcctga acggacatga cggcaacatc ccggcgctgg agatcgcggc cagaaaggtc
     1081 aaggatcgct ttcccgacgc gacgctcgtc tacctgcccg catggtggaa catcaccggc
     1141 gcgaaaatgg cggatgactt cgaggtgtgg aacggcctgg gacacggagg agagggggag
     1201 acctccatca tgatggcggc gcgccccgac ctcgtcgacc tgacacaggc gaagagccag
     1261 gttccccacg atgtgatcgc gctgagcgat tttgcgacgg tcatctggga tatcgccgag
     1321 atcacctcca ctggcgcaac gggggactcc accaaagcga cgcaggaaaa gggagaaaaa
     1381 atgcttcgct gcgtgacgga ctacctggtc ggcctggtgg aggagctgga gcgtcgagga
     1441 tggaaatacg acctgaaaaa atgagatccc ctgcgggaat gtgtccgctt gccggagaga
     1501 ggagctttcg ggttttcccg gaatggactt ctccgcagtg tattgttatg tacgagaacc
     1561 gtgaagggag aacgctctgc catgaatata tcgtccatca ttgaaaaggc gaacgcactg
     1621 acggagaaga tcacggagct gcgcagaatg ttccatgcgc atccggagct cccctggctg
     1681 gagacggaga cgtcgctctt tgtggagcag tatctccgga agctggggct tgagaatatc
     1741 cgaagaggat tcgacggaac gcaatccggc gttgtggccg atctgaaggg cgggaagccg
     1801 ggcagatgcg tcgctctgcg ggccgacatc gatgcgcttc ccttggaaga agagaacgac
     1861 gtcccttata agtcgaccca tagcggagtg atgcacgcct gcggtcatga cgcgcatact
     1921 gcggtcttgc ttggcgtcgc tgagattctg tcctcgatga gggacgagct ttgcggcacg
     1981 gtccgtttcc tctttcaacc cgccgag
//
LOCUS       DAOSWW010000508         2003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46667_length_2003_cov_3.984086, whole genome shotgun sequence.
ACCESSION   DAOSWW010000508 DAOSWW010000000
VERSION     DAOSWW010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46667_length_2003_cov_3.984086"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PK442_09800"
     CDS             complement(<1..707)
                     /locus_tag="PK442_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOO87988.1"
                     /translation="MDLVTQTPRMPKVGETLEARRFHTGFGGKGANQAVALSRLGADV
                     MMVARIGDDMFGAEMKRNFEENGVDARFVEPVPGCSTGVAAISVDDEGNNCIMIVKGA
                     NGTLSPDDVAGAGESLKRSDMLLLQLEISLDTVYAAIDFGAKNGVPVFLNPAPGMPLK
                     REYLRMVDFLAPNETELEIITGMPVASTGDVLAAARSLVNEGVPKVLVTLGAKGSLLV
                     DSKEHLFVDPFPVKPVDT"
     gene            complement(896..>2003)
                     /locus_tag="PK442_09805"
     CDS             complement(896..>2003)
                     /locus_tag="PK442_09805"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOO87989.1"
                     /translation="LAKAFRLLAAEGPAAFYYGPIGEAVVAAVNRAGGDMSMKDLNDY
                     KMIIQEPVMGTYRGYSIYSTPPASSGGTHIVQLLNIMENFPVAQWKHNSPEYLHYLAE
                     AMKMVFADRQRYMADTAFVDVPLAGLASKEYAKQLAEKITRYNVMQEVLPGDPWQFDG
                     GKKTAYIGHDGNKHISTSSFSVVDSDGNIVASTNTVNYFFGSGVIVPEYGFVLNNEMD
                     DFAQDPASVNAPEPGKRPLSSMSPTIVLDPEGRPFMSLGSAGAMRIITAMSQIIMNVV
                     DFGMTMDEAIEQPRIFNVASSGKAGKLLVEGGIDPATVMYLQFRGHNIDERARGGYFG
                     TAQGILINYAKGMHQHGRINGGADSRRLGVPVGF"
BASE COUNT          421 a          586 c          566 g          430 t
ORIGIN      
        1 gtatcaacag gctttacagg gaaggggtcg acgaagagat gctccttcga atcaaccaaa
       61 agcgatccct tcgcgccaag cgttacgagc accttcggaa cgccctcgtt cacaaggctc
      121 cgtgcggctg ccagcacatc tccggtcgat gcgacaggca tcccggtaat aatctcgagc
      181 tctgtttcgt tcggcgcaag gaaatcgacc atccggagat actctctctt gagcggcatt
      241 ccgggggccg gatttaaaaa gacggggacg ccgttcttcg cgccgaagtc aatcgcggcg
      301 tagaccgtat ccaaggaaat ctcgagttgc agaaggagca tatcgctccg tttgagcgac
      361 tcgcccgcac cggcgacatc gtcaggagag agggttccgt tcgctccctt cacgatcatg
      421 atgcagttgt tcccttcgtc atccacggaa attgcagcaa cgccggtaga acacccggga
      481 accggctcca cgaaacgggc gtcgacgccg ttctcctcga agttgcgttt catctccgct
      541 ccgaacatgt cgtccccgat tctggccacc atcatgacgt ctgcgcccaa acgggagagc
      601 gctacggcct ggttggcgcc cttgcctccg aacccggtgt ggaaacgccg agcttccagc
      661 gtctcgccga ccttcggcat acgcggagtt tgtgtcacaa gatccatatt cgcgcttcct
      721 atcactgcga cctttggtac tctttccatg tacactcccc tgccttccgg taacgacgca
      781 tccggaaaac cgagccctgc aagccgaata tcagggaatc agatgcgtct tcgcaatgaa
      841 aaagcggaac cggattatcc cggtcccgcc tgtagtataa cggtaaaaca gatttttaga
      901 aaccgacggg aacgccgaga cgtctgctgt cggcgcctcc gttgatcctg ccgtgctggt
      961 gcatcccctt ggcgtagttg atgagaatgc cctgcgcggt gccgaagtag ccgccgcgag
     1021 cgcgctcgtc aatgttatgg ccgcggaact ggaggtacat aaccgtcgcc ggatcgatgc
     1081 cgccttcaac gaggagtttg cccgctttgc cactactcgc aacgttgaaa atcctcggct
     1141 gctcgattgc ctcgtccatg gtcattccga agtcgacaac gttcatgatg atctggctca
     1201 tagcggtgat gattctcatg gctcccgcgg aaccgaggga catgaagggt ctgccctcgg
     1261 ggtcgagaac gatcgtcggc gacatcgaag aaaggggacg cttgccgggt tcgggcgcgt
     1321 tgacgctcgc cggatcctga gcgaaatcgt ccatctcgtt gttcagaacg aaaccgtact
     1381 cagggacgat cacgccggaa ccgaagaagt agttcacggt attcgtggag gcgacgatgt
     1441 tcccgtcgct atcgacaacg gagaaggaac tcgtggagat gtgcttgttc ccgtcgtggc
     1501 cgatgtaggc ggtcttcttg cctccgtcga actgccacgg atctccggga agaacttcct
     1561 gcatcacgtt gtagcgggta attttctcag cgagctgctt cgcgtactcc ttgctcgcga
     1621 ggcctgcaag aggaacgtcc acgaacgcag tgtccgccat gtatctctgg cggtctgcaa
     1681 agaccatctt catcgcctcg gcaaggtagt gaagatattc gggagagttg tgcttccact
     1741 gagctaccgg gaaattctcc ataatgttga gaagctggac gatgtgcgtt ccgccgctgg
     1801 atgccggagg cgtggagtag atggagtatc cgcggtacgt acccataaca ggttcctgga
     1861 tgatcatctt ataatcgttc aggtccttca tggacatgtc gccccccgca cggttcaccg
     1921 cagcgacaac agcttcgccg atagggccgt aatagaaagc ggcggggcct tccgcggcaa
     1981 gcagacggaa tgcttttgcc agc
//
LOCUS       DAOSWW010000509         2002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46708_length_2002_cov_3.229070, whole genome shotgun sequence.
ACCESSION   DAOSWW010000509 DAOSWW010000000
VERSION     DAOSWW010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46708_length_2002_cov_3.229070"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PK442_09810"
     CDS             complement(<1..352)
                     /locus_tag="PK442_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HOO87990.1"
                     /translation="MRRTLLSVLCTCALLLLLLPALASGTEKIVVEAEGMAAIANGDV
                     ARAREEARRHLYRNALEKGIGARVQGITEMKDFEVVRDTVFSRSEGLVTATENLKEER
                     TNDGILRLTAQCTVS"
     gene            complement(357..1289)
                     /locus_tag="PK442_09815"
     CDS             complement(357..1289)
                     /locus_tag="PK442_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87991.1"
                     /translation="MSQKRFVSCLLFLLLLGVPAFATEEVFLPPIEDLPEAGDSVTAA
                     PAVDVSTAFDTGALPAPSPSALIVGTEFARVNWTDDYVEASGQAVAPTGKEKTAQGKL
                     LARRGAIVDLQRNLLEAIQGVRVDAKTTMTDFMANDTVRTEVQGLIKGIEISDASWDG
                     EIYTVTGRVKLEKVRTAVLPGLPKAPGQKPVPPTPPAGKISASGLLIDATHLPLIPAM
                     IFRVVDENGKEVYGIDFADAERFLASGLCDYHTNLAYAKGAPRIASTPLVAKAVRTVA
                     PQNVDIVISNADAAKVRGSSYDFRVPCRVTVVKR"
     gene            complement(1515..>2002)
                     /locus_tag="PK442_09820"
     CDS             complement(1515..>2002)
                     /locus_tag="PK442_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOO87992.1"
                     /translation="DQIEAMSISDRVVVMNRGRIEQMGSPRELYARPINRFVASFIGK
                     AAFLPAEKNASGAWTLLGKPWRPEAGEELTAGAYEAMIRPEAVRLSPLENSGRNAIAG
                     TVLRATYLGSVMEYEVELPGTEPVTVHIPNPFERDPLPPGAEAELRFLPEGLHFLPAE
                     A"
BASE COUNT          363 a          599 c          627 g          413 t
ORIGIN      
        1 cggagactgt gcactgcgcg gtcaggcgaa gaatgccgtc gttggtccgt tcttccttca
       61 aattctccgt ggccgtcacc agcccctccg aacgggaaaa gaccgtgtcg cggaccacct
      121 cgaagtcttt catctcggtg atgccctgaa cccgtgcgcc gattcccttt tcgagcgcgt
      181 ttctgtagag gtgacgccga gcttcctccc gcgcccgagc gacgtcgccg ttcgcaatcg
      241 cggccattcc ctccgcttcg acgacgatct tctccgttcc ggaggcgagc gccgggagaa
      301 ggagcagaag aagcgcgcag gtacacagta cggagaggag tgtccgtctc ataatgtcac
      361 cgtttaacca ctgtcacgcg gcagggaacg cgaaagtcgt acgagctgcc gcgcaccttc
      421 gcagcgtccg cgttgctgat gacgatatcg acgttctgcg gcgcgaccgt ccgcaccgct
      481 tttgcgacga gaggtgtgga ggcgattcgc ggagcgccct tcgcataggc gaggtttgtg
      541 tggtagtcgc acagaccgga ggcgaggaag cgctccgcgt cggcaaagtc gataccgtac
      601 acttccttgc cgttttcgtc gacgacgcgg aagatcatcg ccgggatgag agggagatgc
      661 gtcgcgtcga tcagcagccc cgatgcgctg atcttgcctg cgggaggtgt tgggggaacg
      721 ggtttttgtc ccggagcctt gggaagtccc ggcagcacgg cggtgcgcac tttttcgagt
      781 ttcacgcgtc ctgtgacggt gtagatctcg ccgtcccagg aggcgtcgga aatctcgatt
      841 cccttgatga gcccctgaac ctccgtccgt acggtatcgt tcgccatgaa gtcggtcatc
      901 gtcgtcttcg cgtccacgcg cacgccctgg attgcctcaa ggagattgcg ctggaggtcg
      961 acgatggccc ccctgcgcgc aagcagtttt ccctgcgcgg tcttttcctt gccggtcggg
     1021 gcgacggcct gtcccgaggc ctcgacgtaa tcgtcggtcc agttgacgcg ggcgaattcg
     1081 gttccgacga tcagcgcgga cggggatgga gcggggagcg cccccgtatc gaaggccgtg
     1141 gaaacgtcga ccgcgggcgc agccgttacg gaatctcctg cctcaggcag gtcttcgatc
     1201 ggcggaagga acacctcctc cgtggcaaac gccggaactc ctaagagcaa caggaacaga
     1261 agacaagaaa cgaagcgttt ttgagacatc gcatactcct cctctctagt ttttcgagga
     1321 agatgggccg aggtttatgt gcggagagaa aagaaaatgt ataccattct cgcgtcttcc
     1381 tcgtacacta tgatactcta tttacttcga attttcaacc gaagcgcgat gttgctttaa
     1441 taggaaaaaa ggctgaagcg ccttccttaa gaagcgcttc agcccgtaat ttctcgcagg
     1501 aggtttttct ggcgctacgc ttccgcagga agaaagtgca gcccctcggg gagaaaacgc
     1561 agctccgcct ccgctccggg ggggagcggg tcgcgctcga acgggttcgg aatgtgcacc
     1621 gtaaccggtt cggtcccggg cagctccacc tcgtactcca tcacgcttcc caagtaggtg
     1681 gcgcggagca cggttccggc tatcgcgttc ctgccggagt tttcgagagg ggagaggcgc
     1741 acggcctcgg ggcggatcat cgcctcgtag gcgccggccg tcagttcttc tccggcttcg
     1801 gggcgccacg gcttccccag aagcgtccac gcgccggaag cgtttttttc ggccggaaga
     1861 aaggccgcct tgccgatgaa ggaggcgacg aagcggttga tagggcgtgc gtacagctcc
     1921 cgcgggcttc ccatctgttc gatgcgcccc cggttcatca cgacgacgcg gtcggagatg
     1981 ctcattgcct cgatctggtc gt
//
LOCUS       DAOSWW010000510         1999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46819_length_1999_cov_3.223251, whole genome shotgun sequence.
ACCESSION   DAOSWW010000510 DAOSWW010000000
VERSION     DAOSWW010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46819_length_1999_cov_3.223251"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PK442_09825"
     CDS             complement(<1..924)
                     /locus_tag="PK442_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HOO87993.1"
                     /translation="MKSLGDMLLNTGPFSEIVLGNTALARAMIESGTEVIASYPGSPT
                     PEIGAALMSVPKERRPYYFEFSTNEKVATEVACGASLNGRPSCVFFKSVGLNVAADSF
                     VQLSLLELNGGLVAILGDDPGANSSQNEQDNRHIARLAYIPVLEPASPAEAYTFYLEA
                     MKLSGKRKMPVILRMTTHVCHGKEKVSFAGWTPRPIGSVPAFDSSAGPSIPLTTRALD
                     MKRRAIARLAEVAQETDGSPLHELREGNDPSKGVITAGLPFRSLVDVLEDAAEKPDIL
                     KLGVVHPLPKGLVADFLRSHASVKVLEELDDV"
     gene            complement(942..1124)
                     /locus_tag="PK442_09830"
     CDS             complement(942..1124)
                     /locus_tag="PK442_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87994.1"
                     /translation="MESGDPLDSDEFLPLKDESGKVTILVPKSMKFTDDKVLVEHFTY
                     RGKDVFICRTALLPDR"
     gene            complement(1146..1313)
                     /locus_tag="PK442_09835"
     CDS             complement(1146..1313)
                     /locus_tag="PK442_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87995.1"
                     /translation="MKKRIVLLGIAALLMVFSLSAAAQELHLALEQSALEKLSAKEGA
                     LYIEWGVYRNT"
     gene            1557..>1999
                     /locus_tag="PK442_09840"
     CDS             1557..>1999
                     /locus_tag="PK442_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87996.1"
                     /translation="MLFLPDSISFPERKPKVWIWDDLYRALAEALGVKPRVQIDPPDH
                     WLLVRHIVRRLRDRCAGALPAGTASPRFLELAGQSIRELLREDVPREELAKALGCGGC
                     APGARCEHLSDEFKASRSERTSDAGSTLLCTLYGDYTALLEERGLA"
BASE COUNT          358 a          623 c          644 g          374 t
ORIGIN      
        1 cacgtcgtcc agctcttcca gcaccttcac ggaggcgtgg gagcgcagga agtcggccac
       61 gagtcccttc gggagagggt gcaccacgcc gagcttgagg atatccggct tttccgccgc
      121 gtcctccagc acgtcgacca gcgagcggaa gggaagcccc gccgtgatga cgcccttcga
      181 cggatcgttc ccctcgcgca gttcgtggag cggcgagccg tcggtctcct gcgccacttc
      241 ggccagacgg gcgatcgcgc gccgtttcat gtcgagcgcg cgcgtggtga gcggaatgga
      301 cggccctgcg gaggagtcga acgcgggaac gctcccgatc ggacggggcg tccaccctgc
      361 gaaggagacc ttctccttgc cgtggcagac gtgcgtggtc atccggagga tgacgggcat
      421 cttccgcttt ccggagagct tcatcgcctc aaggtagaat gtgtaggcct ccgcaggcga
      481 ggcgggttcg agcacgggaa tgtaggcgag gcgggcgatg tggcggttgt cctgctcgtt
      541 ctgcgaagag ttcgcgccgg ggtcgtcgcc gaggatggcg accaggccgc cgttcagctc
      601 cagtagcgag agctggacga aggagtcggc cgcgacgttg agaccgacgc ttttgaaaaa
      661 gacgcacgag gggcgcccgt tgagcgacgc gccgcacgcg acctcggtgg ccaccttctc
      721 gttggtggag aactcgaagt agtagggacg gcgctctttg ggaacgctca tgagcgcggc
      781 cccgatctcc ggcgtggggg agccgggata ggaggcgata acctccgtcc cggactcgat
      841 cattgcgcgg gccagcgcgg tgttcccgag gactatttca ctgaacggcc cggtattcag
      901 cagcatgtcg ccgagacttt tcatttgagg acctccttcg tttaccggtc gggcaggaga
      961 gccgtcctgc agatgaaaac atcctttccg cgataggtga agtgctccac aagcactttg
     1021 tcgtcggtga acttcatgct cttgggcaca agaatggtca ccttgccgga ttcgtccttc
     1081 agcggaagga attcatcgga gtcgagggga tcgccgctct ccacgagagc ggatggcttc
     1141 tcccctcagg tgttgcggta gacgccccat tcgatgtaga gcgctccttc tttcgccgac
     1201 aacttttcga gcgcgctctg ctccagcgca aggtgaagct cctgcgctgc cgccgacaga
     1261 gagaacacca tcagcaacgc cgctataccc agcaatacga tacgtttttt catgaacgaa
     1321 gatctcctcc tcttataaaa tgaaacagat tctctccggg aattgtatca cggactgcgt
     1381 gttgagtgct acacttgtcg cgagccgtga aatgatcgca cgaggaggcg ttccccctga
     1441 gactccacac ctaccgcacc ccccgaagcc ttgcagaacc gctgcgcgaa gaggccgcgc
     1501 gcggcgacac gatctttctg acgccgtcga acagggaccg tccgctgctg ctggagatgc
     1561 tcttccttcc ggactcgatc tccttcccgg agcggaagcc gaaggtgtgg atatgggacg
     1621 acctctaccg ggcgctcgcc gaggcccttg gcgtgaaacc ccgcgtgcag atcgaccctc
     1681 ccgaccactg gctgctcgtc cggcacatcg tccgtcggct tcgggaccgc tgcgccggcg
     1741 cgctgcccgc cgggacggcc tctccgcgct tcttggagct cgcgggacag tccatccgcg
     1801 aactgctcag ggaagacgtc ccgagggagg aactggcgaa ggcgctgggc tgcggcggct
     1861 gtgctccggg agcgcgatgc gagcatttga gcgatgaatt caaagcctcc cggagcgagc
     1921 gtacgagcga tgcggggagc acgcttctct gcacgctcta cggcgactac accgcacttc
     1981 ttgaagaacg cggtcttgc
//
LOCUS       DAOSWW010000511         1999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46822_length_1999_cov_3.046811, whole genome shotgun sequence.
ACCESSION   DAOSWW010000511 DAOSWW010000000
VERSION     DAOSWW010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46822_length_1999_cov_3.046811"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..232)
                     /locus_tag="PK442_09845"
     CDS             complement(8..232)
                     /locus_tag="PK442_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87997.1"
                     /translation="MQTSLQERKQLYRRIRGLSEDKYAKVVKFVLSMENEEPPLSAEE
                     EEDLRLAYAQMKSGDYRSFDEAMKELWESE"
     gene            complement(445..1200)
                     /locus_tag="PK442_09850"
     CDS             complement(445..1200)
                     /locus_tag="PK442_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO87998.1"
                     /translation="MAFNRKQIIRGLLVCVLFFSLSAAAFADAKVLARWSQTKSFKSD
                     LGSELDVRATYYSAEYIEALVKQEAEKNLWTENETEQYKYQLLSGLGLDEHIPIHVEF
                     DNRGPSMHLAPFDSFLTLWVGKNKLPPVDFDKRFNFSFQGKREGLVFFPRYDNKGKSY
                     LEGVKTIRFTMAGAISSVTINLSTIDFVWDVARDNPEALYKGRAAARLELDRLIKRIE
                     KLNAEKRELEGKLSELNAELDTITHRVEELQRQ"
     gene            complement(1328..>1999)
                     /locus_tag="PK442_09855"
     CDS             complement(1328..>1999)
                     /locus_tag="PK442_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HOO87999.1"
                     /translation="FEIGARAKFNFNEQLWLSLNGMMRMYDSNVLTSLTPAGVAKEDE
                     HIYWGAVGINFTPSIALKAAYYGQEVQVGDNVGTESPNSFQAIIDVKQEALKFTSLWA
                     EYRSFDDNFTTWMDGGPWDNYGAAVTGPVVNGMVNIGSLYDSAIFIKLNQKWNDQWST
                     FQRYLVANPRAGGLDDTTNWTFGVKYNYTPALSFELAYDNIENAGTVVNDDDSLIRFR
                     THIAF"
BASE COUNT          441 a          544 c          515 g          499 t
ORIGIN      
        1 cgtccactca ttcactctcc caaagctcct tcatcgcttc atcgaaagag cgataatctc
       61 ccgacttcat ttgagcatac gcaagacgaa gatcctcctc ctcctcggcg gaaagaggag
      121 gttcttcatt ttccatagag agaacgaatt ttactacttt cgcgtacttg tcttctgaga
      181 gcccgcgaat tcttctgtac aactgtttgc gttcttgcag actggtttgc atagtcaaat
      241 cacactcctg ctcccggtct cgacttaaga atttcctcta ccctcaaagt atagcacgga
      301 gaaaagggaa ggagcgcgga tcgggatgtc cgcgctcctt ccctttttgg ttcgctcggt
      361 gttcggcgtt caataagcct tttgccccat tccgttcgac ggaagggcga gcgaaaaggc
      421 gtgcgtagtg tcgtttccgg cgccttactg gcgctgaagc tcctcgacgc gatgggtgat
      481 ggtatccagc tcggcgttca gctcgctcag cttgccctcg agttcccgct tctcggcgtt
      541 gagcttctcg atgcgcttga tcaggcggtc gagttcgaga cgggctgcgg ctcggccctt
      601 gtacagagct tcgggattgt cgcgggcgac gtcccaaaca aaatcaatgg tcgacagatt
      661 gatcgtcacg ctgctgatgg cgcccgccat ggtgaatcga attgttttta ccccttcaag
      721 ataggacttc cccttattgt cataccgcgg gaaaaaaacg agcccttctc ttttgccctg
      781 aaaactgaag ttgaaacgct tgtcgaaatc gacagggggc agtttgttct ttccaaccca
      841 cagagtgagg aacgagtcga aaggagcaag gtgcatcgag ggacctctat tgtcgaactc
      901 gacatgaatg ggaatatgct cgtcgagacc gagtccgctc aacagctggt acttgtactg
      961 ctcagtctcg ttttcggtcc agaggttctt ctccgcctcc tgcttcacga gcgcctcgat
     1021 gtactccgcg gagtaatagg tcgcgcgaac gtccagttcg cttcccaaat cgctcttaaa
     1081 agacttcgtc tggctccaac gcgccaacac cttcgcgtcg gcgaaagccg cggcggacaa
     1141 ggagaagaaa agcacacaca caaggagccc ccgaattatt tgttttctgt tgaacgccac
     1201 acataacgcc ccctcttgaa taagcttccc ggcaattata caggagatat aaaaaaagag
     1261 gaaccctttc gggctcctct tttatcgtgt ctggtacaat gcttctgaac ccgacgactt
     1321 ggtttgttta gaaagcgatg tgcgttctga agcggatcag gctgtcatca tcgttaacaa
     1381 cagtaccagc gttttcgata ttatcgtagg caagctcgaa ggaaagagca ggagtatagt
     1441 tgtacttcac gccgaacgtc cagtttgtgg tgtcatcaag tcccccagcg cggggattcg
     1501 ctaccaagta gcgctggaag gtgctccact gatcgttcca cttctggttc agtttgatga
     1561 agatagcgct gtcgtacaga gagccgatgt tcaccatgcc attgactaca ggaccggtga
     1621 cggctgcacc gtagttatcc cacgggccac catccatcca tgtggtgaag ttgtcgtcga
     1681 agcttctgta ttcagcccag agggaagtga acttcagcgc ttcctgtttg acgtcgatga
     1741 ttgcctggaa ggagttcggg gactctgtac ctacattgtc gcccacctga acttcctgac
     1801 cgtagtacgc agccttcagc gcgatggaag gcgtaaagtt gatgcccacc gcgccccagt
     1861 agatgtgctc gtcttccttg gctacgccgg cgggcgtaag ggaagtaagc acgttggagt
     1921 cgtacattct catcatgccg ttgagcgaga gccacagctg ctcgttgaag ttgaacttcg
     1981 cgcgcgcgcc gatttcaaa
//
LOCUS       DAOSWW010000512         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46946_length_1996_cov_5.009789, whole genome shotgun sequence.
ACCESSION   DAOSWW010000512 DAOSWW010000000
VERSION     DAOSWW010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46946_length_1996_cov_5.009789"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PK442_09860"
     CDS             complement(<1..688)
                     /locus_tag="PK442_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOO88000.1"
                     /translation="MLRRDRWRLGIVAIVVLAALFSVFPIQGRINLGLDLKGGAHIVL
                     QAKDTADSKVTEDSIERLLAVLRNRVDQYGVAEPVIQREGTDRIIVDLPGIQDPDAAL
                     ELIGKTALLEFRQVVTATSPVPPSAERKNYDSDAEFERAQTRWNEAVAKIEAQKGELE
                     QQAASLEGGVVAKADEGRFYLLGKVLVGGKDLVDARTQYDNLGRAVVAISFSSEGAKL
                     FDAATAENIGR"
     gene            complement(879..1238)
                     /gene="yajC"
                     /locus_tag="PK442_09865"
     CDS             complement(879..1238)
                     /gene="yajC"
                     /locus_tag="PK442_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOO88001.1"
                     /translation="MEQQCGQAAGGAGGQGSLMGMLFPLAIFVVIFYFFIIRPQKKRQ
                     RAQDELLSSLSRGDQVVTIGGFFGTIREVRDDSLVVEIADGVKVRLLKSAVASKRAPA
                     TQAPKEEPAREKEEEKN"
     gene            complement(1330..1965)
                     /locus_tag="PK442_09870"
     CDS             complement(1330..1965)
                     /locus_tag="PK442_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOO88002.1"
                     /translation="MKDTKIAEPTVERLVQYHRLLEQLAAEGQKVVSSREMGEMLSFK
                     ASQVRKDLSYFGEIGKRGVGYHVDRLFKHIGGILASPRLWSIGLAGVGKLGEALLGYQ
                     AFRNEKFQIKALFDVDPSKIGTHIQGTPCYSIEDAPRVLREEGIDVVIITVPTGAAQS
                     VADRVVSAGTVKGILNFSPVTLSVPDSVLLYSVDISIELEKLLFYLKHRER"
BASE COUNT          412 a          631 c          540 g          413 t
ORIGIN      
        1 gccgtccgat gttctcggcc gtcgcagcgt caaagagctt cgcgccttcg gagctgaacg
       61 atatggcgac gactgcgcgt ccgaggttgt cgtactgcgt cctggcgtcc acgagatctt
      121 ttccgcctac cagaactttc ccgagaagat aaaacctgcc ctcgtctgct tttgctacca
      181 cgccgccctc aagagaagcg gcctgctgtt caagttctcc cttttgggcc tcaatcttcg
      241 ctacagcctc gttccaacgc gtctgcgccc gctcgaactc ggcgtcgctg tcgtagttct
      301 tccgctctgc ggagggagga accggcgacg tcgccgtcac aacctggcgg aactcaagaa
      361 gcgccgtctt gccgataagc tccaatgccg cgtccggatc ctggataccg gggaggtcga
      421 cgatgatcct gtcggtaccc tccctctgga tgacgggttc cgccacaccg tactggtcca
      481 cgcgattccg aagcacagca agaagacgtt cgatgctgtc ctccgtcacc ttgctgtccg
      541 cggtgtcctt cgcctgaaga acgatgtggg cgccgccctt caaatctagc cccagattga
      601 tccttccctg aatgggaaag accgagaaca gggccgcaag caccacgatg gcgactattc
      661 caagccgcca gcggtctcgt ctcaacataa gaattccctc cgttgtgttc gcaaaattgg
      721 ttgttcattc ccccgcgaaa aaactcccgc caacagtatg gccgacggga cgcgagcggg
      781 gggatcgcct cgcggcgacc tccccccgtt tccccgccgc acctgcggtt gcatcggaag
      841 ggggcggggc gcacttcaca tcgaaccggc agatacggtc agttcttctc ctcttccttc
      901 tcgcgtgcgg gctcctcctt gggagcctgg gtggcgggag cgcgcttcga agccaccgcc
      961 gacttgagaa ggcgtacctt cacgccgtcg gctatttcga cgacaaggct gtcgtcgcga
     1021 acttcacgga tggttccgaa gaaaccgccg atggtgacca cctggtcccc cctcgaaagc
     1081 gatgagagaa gttcgtcctg ggcacgctgc cgcttcttct ggggacggat gatgaagaag
     1141 tagaaaatga ccacgaatat cgcgagaggg aagagcattc ccatgagact tccctgtcct
     1201 cctgcgccgc ctgccgcctg tccacactgc tgttccaaaa aaaacgcctc cttgatatta
     1261 gggtgcaagg ttgcctcgcc ctgcccggca agacggaaaa caaccccgat attctatcaa
     1321 aaaaaactgc taacgttcgc gatgcttcag gtaaaaaaga agtttctcca gttctatcga
     1381 aatatccacg ctgtagagca gcacgctgtc cggaacggag agcgtgaccg gggagaagtt
     1441 gagtatcccc ttcaccgttc ctgcagaaac gaccctgtct gcaacggact gggccgctcc
     1501 cgtcggcacg gtgatgatga cgacatcgat accttcctct cgcagcacgc ggggagcgtc
     1561 ctctatggag tagcagggag ttccctggat atgcgttccg attttcgaag ggtcgacgtc
     1621 gaagagggcc ttaatctgaa acttctcgtt ccggaacgcc tgatacccta aaagcgcctc
     1681 gccgagtttt cctacgccgg ccagaccgat ggaccagagc ctgggggacg caaggattcc
     1741 gccgatatgc ttgaataaac gatcaacatg ataccccacg ccccgcttgc ctatctcgcc
     1801 gaagtacgag aggtccttcc gcacctggct ggccttgaac gagagcatct cccccatctc
     1861 cctggaggag accactttct gcccctcggc cgcgagctgc tctaaaagac ggtgatactg
     1921 gacaagccgc tcgactgtgg gctccgctat cttcgtatcc ttcatgtgcg cacatgaagg
     1981 atacgaagat agcgga
//
LOCUS       DAOSWW010000513         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46949_length_1996_cov_3.746522, whole genome shotgun sequence.
ACCESSION   DAOSWW010000513 DAOSWW010000000
VERSION     DAOSWW010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46949_length_1996_cov_3.746522"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..812)
                     /gene="kdsB"
                     /locus_tag="PK442_09875"
     CDS             complement(99..812)
                     /gene="kdsB"
                     /locus_tag="PK442_09875"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOO88003.1"
                     /translation="MNALAVIPARYASTRLPGKPLLPVAGVPLVLRVLEQVQKCSGVD
                     RIIVATDDQRIADVVENAGAEALLTPPELPSGGDRVAWVSERIPSKIVLNIQVDDPLV
                     GPDMIDPLIAALSDEGTALALLAKRIENEREIESPNIVKMVFDKNQRALYFSRSPIPY
                     PRTSGAAYYKHIGPYAWKREFLLQFSGWNQTPLEKSESLEMLRVLENGYSIRCIPVAR
                     DTIEIDTPEDVAAFEALLA"
     gene            complement(947..1888)
                     /gene="arcC"
                     /locus_tag="PK442_09880"
     CDS             complement(947..1888)
                     /gene="arcC"
                     /locus_tag="PK442_09880"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOO88004.1"
                     /translation="MRVLVALGGNAILQRGQKGNASEQRANVQKTVTQIVRMIHAGHE
                     VVVTHGNGPQVGAILIQNELGSASVPAMPMDICGAESQGLIGYMFCQSIQNTLLADGI
                     EGHAPACLVTRVEVDPADPAFTHPTKPVGPFYTEEAAKKRIAETGETWIDDAGRGWRR
                     VVPSPDPKSIVEADVINTLLEKGFTVVASGGGGIPVIRKENGTWEGVEAVIDKDLAGE
                     RLAASVKADLLMILTDVPKVAIHYNTPQQKWLDKLTLSEMESLEKEGHFKAGSMGPKV
                     KAALRFVRNGGQRAVIASLDEALEALEGTKGTQILKG"
BASE COUNT          410 a          554 c          533 g          499 t
ORIGIN      
        1 cgcgattcgt ccggccaaaa acatgttgaa ttgcatcttt ccacctctaa tttccttgcg
       61 gctgttgccg cggtctctgt ggttcgtcga tcattccgtc aggcgagcag cgcttcaaac
      121 gccgcaacgt cctcaggagt gtcgatttct atagtgtccc gtgcaacggg aatacagcgg
      181 attgagtacc cgttttccag aacccgcagc atctcaaggc tttctgattt ttcaagcgga
      241 gtctggttcc agccggagaa ttgaagcaaa aattctcgtt tccaggcgta cgggccgata
      301 tgtttgtagt atgccgctcc ggaggtgcgg gggtagggga tgggagacct gctgaagtag
      361 agcgctctct ggttcttgtc gaagaccatt tttactatgt tcggcgattc tatctcccgt
      421 tcgttctcta ttcgcttggc aagcagtgca agcgccgtgc cttcgtccga gagcgccgca
      481 atcagcgggt cgatcatgtc cgggccgaca agcggatcat cgacctggat attcaaaacg
      541 atctttgaag gaattcgttc ggagacccat gcgacacggt cgcctccgct tggcagctca
      601 ggcggagtga gcagagcttc cgctccggcg ttttcgacga catcggcaat tcgttggtcg
      661 tcagtggcaa cgatgatgcg gtcgacgccg ctgcactttt gaacctgttc cagaactctg
      721 aggacgagcg gtacgcctgc gactggaaga aggggcttcc ccgggagacg agtgctcgcg
      781 taccgggccg gaatgactgc aagagcgttc ataaatccat ctccttgccg gaaaaaaacc
      841 ggaagtaaat tctacatcat ttattatcac ctccatagac tctttttcgc atcaacaaaa
      901 aacctccctc ttgagtgagg gaggtttata tcgcgaagat aaaaaactaa cctttaagaa
      961 tctgggtacc cttggttcct tcgagggctt ccagagcttc atcgaggctt gcgatcaccg
     1021 cgcgctgtcc gccgttacgg acgaaccgga gtgcagcttt tacttttggc cccatggaac
     1081 cggccttgaa atgcccttct ttttcgagag actccatctc ggagagcgtc agcttgtcga
     1141 gccacttctg ctgcggtgta ttgtagtgaa tggctacctt gggaacatcg gtgagaatca
     1201 tcagcagatc cgccttcaca gaagcggcaa ggcgttcgcc tgcaagatcc ttgtcgatga
     1261 cggcttccac accctcccat gttccgtttt ccttccggat gacgggaata ccgccgccgc
     1321 cggaggcaac tactgtgaat cctttttcaa ggagcgtgtt gatgacgtcc gcttcgacga
     1381 tcgacttggg atccggggag gggacaaccc ggcgccatcc tcgtcccgca tcgtcgatcc
     1441 atgtctctcc ggtctcggca atgcgcttct tagcggcctc ttcagtatag aagggaccca
     1501 ccggctttgt gggatgggta aacgccggat ccgcaggatc cacttcgact cgggtaacaa
     1561 ggcaagcagg cgcgtgtcct tcgatgccgt ccgcaagcag ggtattctgt atgctctggc
     1621 agaacatgta tccgatgagc ccctggctct cggcgccgca tatatccatg ggcatcgcgg
     1681 gaacagaggc gctcccgagt tcgttctgta tgagaattgc tccgacctga gggccgtttc
     1741 cgtgcgtcac gaccacttcg tgcccggcat ggatcattct gacaatttgc gtcacggtct
     1801 tttggacgtt cgcacgctgt tccgaggcgt ttcccttttg ccctctttga agaatggcat
     1861 tgcccccaag ggcgaccaat actctcatct ctgcaaaatt ccctcctgaa tgtcgtagtt
     1921 gacttctata ttatagagta tgcgccttat ttcagcaagt ctttaacaaa cggcggaagc
     1981 acgaaagccg ctttgt
//
LOCUS       DAOSWW010000514         1996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_46951_length_1996_cov_3.696548, whole genome shotgun sequence.
ACCESSION   DAOSWW010000514 DAOSWW010000000
VERSION     DAOSWW010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46951_length_1996_cov_3.696548"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /gene="cbiE"
                     /locus_tag="PK442_09885"
     CDS             complement(<1..529)
                     /gene="cbiE"
                     /locus_tag="PK442_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HOO88005.1"
                     /translation="MGRFTVVGLGPGDDSCLLPCARRAIEDAEILAGARRHLEPYEKS
                     GKTLLFLEKGVDELLDAVDKHRKLGRVALLLSGDPCLFSLLGRIAARFPSGDYEVVPG
                     LSSFQLLFARLASLPGEPGAPSPSWSDVRLESIHGRPIEGVASMLHEHRRTLLFLDSK
                     NSGPAVAAFLRDRGFP"
     gene            complement(502..726)
                     /locus_tag="PK442_09890"
     CDS             complement(502..726)
                     /locus_tag="PK442_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88006.1"
                     /translation="MLAERMHRRCALYAISRASLVEPPQKSTETRATPSGNTVAFGVA
                     VLSLKGENLGSFPSLSLKEGSSWEDLPSSV"
     gene            complement(1037..>1996)
                     /gene="cbiD"
                     /locus_tag="PK442_09895"
     CDS             complement(1037..>1996)
                     /gene="cbiD"
                     /locus_tag="PK442_09895"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HOO88007.1"
                     /translation="SGTTRARLAMRKALPYLDGAIVAIGNAPTALFEVCDLIRRGECR
                     PALVVGVPIGFVGAAESHDELASLDCEWITASGPKGGSPVAAAVVNAIIRLALKDAPP
                     APEKGGRRGFTTGSCAAAAAKAGATLLVTGEAPCFAELRLPSGGAVLRIPVEACELSP
                     SGARCSVRKDAGDDPDVTDGMLIVAEVALSEEPGITISGGRGIGRVTRKGLPLPPGEW
                     AINPTPRTMIRDALETLLPPEKGLSVTISAPEGEERAAKTWNPRLGIEGGISIRHNWH
                     CRAEVYGRVPRLHRPVHLGGARLRLPPAGNHLLRPRLRRRKDA"
BASE COUNT          322 a          670 c          697 g          307 t
ORIGIN      
        1 cgggaaagcc cctgtcccgc agaaacgccg ccacggcagg gccggagttc ttcgagtcga
       61 ggaagagcag cgttctcctg tgctcatgga gcatcgaggc gaccccttcg atggggcgtc
      121 cgtggatgct ttcaagacgc acatcgctcc acgaggggct cggagcgccc ggttcccccg
      181 gaagcgacgc gagtcgggcg aaaaggagct ggaagctgct caggccggga acgacctcgt
      241 agtcgccgga gggaaaacgg gccgcgatcc gtccgaggag gctgaaaagg cagggatcgc
      301 ccgagagcag cagcgcgacc cgaccgagtt tccggtgttt gtccaccgcg tcgaggagct
      361 cgtccacgcc cttctccaga aagaggagcg tcttgccgct cttctcgtac ggctcaagat
      421 gccgccgtgc tccggcgagg atctccgcgt cctcgatcgc gcgccgggcg caggggagca
      481 ggcaggagtc gtctccgggg cctagaccga cgacggtaaa tcttcccatg acgacccctc
      541 cttcagcgaa agagagggaa agctccccag attctccccc ttgagcgaga gcaccgcgac
      601 gccgaaggcg acggtgttcc cggagggcgt cgcgcgcgtc tccgtgcttt tctgcggcgg
      661 ctcgaccaga gaggcgcggg agatggcgta cagggcgcac cggcggtgca tccgctccgc
      721 cagcacgtcg aaggtcttcg aaagccccca gcgctcgatc agcggaaccg cctcctccgc
      781 cagcggcagt gcgagcagtt cgcggatctg ttcgggcgtt gcgccgcaag cgcccgcgca
      841 cgccgcaagc gtttcgagcc tcgcgtcgcc gtagtcgcag tgcgtgttga acagcccggc
      901 ggcgaccttt gcccacttac cgacgtgccc gaagaggtgg aggcggcgcg cgcccagctc
      961 gacgctcttc ttcagcgcgt gcccgaagtg gtcccccgtg cggacgatca gctcgcgggg
     1021 aacggcgtag cgttcttcaa gcgtcttttc tccgacgtag ccggggacga agaagatggt
     1081 tcccggccgg ggggagtcga aggcgagcgc cgccgagatg tacaggtcga tggaggcgag
     1141 gtacgcggcc gtagacttcg gctcgacaat gccagttgtg ccggatggag atccccccct
     1201 cgatgccgag gcgcgggttc cacgtcttcg cggcccgttc ctccccctcg ggggcggaga
     1261 tcgtcacgga cagccccttt tccgggggaa gcagcgtttc gagcgcgtcg cggatcatcg
     1321 tccggggagt cgggttgatc gcccactctc cgggagggag ggggagcccc ttccgtgtga
     1381 cgcgcccgat gccccggcct ccggagatcg tgattccggg ctcttcggag agcgcgacct
     1441 ctgcgacgat gagcatgccg tcggtgacgt ccgggtcgtc cccagcgtct ttgcggacag
     1501 aacagcgggc tcccgatggg gagagctcgc acgcttccac ggggatgcgc agcacggctc
     1561 cgccggaagg aagacggagc tccgcgaagc aaggggcctc cccggtgacg agcagcgttg
     1621 ccccagcctt cgccgccgcc gccgcgcagc ttccggtcgt aaaacctcgc ctgccgccct
     1681 tttctggagc cgggggagcg tccttgagcg cgaggcggat aatagcgttc accacggccg
     1741 cggccacggg gcttccgccc ttcggaccgg aggcggtgat ccattcgcag tcgagcgacg
     1801 cgagctcgtc gtgcgactcc gccgcgccga cgaagccgat ggggacgccg acgacgagcg
     1861 ccgggcggca ctccccccgc cggatcagat cgcagacctc gaagagggct gtcggcgcgt
     1921 tgccgatggc cacgatcgcg ccgtcgagat acggaagagc cttccgcatc gccaggcgcg
     1981 cgcgggttgt tccgct
//
LOCUS       DAOSWW010000515         1993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47090_length_1993_cov_3.694530, whole genome shotgun sequence.
ACCESSION   DAOSWW010000515 DAOSWW010000000
VERSION     DAOSWW010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47090_length_1993_cov_3.694530"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PK442_09900"
     CDS             complement(<1..266)
                     /locus_tag="PK442_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOO88008.1"
                     /translation="MTSIRAAVFKMRGGIWTVLFLAVWFLAEPPGPYRSFLGLVLVAL
                     GQMLRFWAAGCITRYRGENVGAARLTTWGPYGIVRNPLYVGNWL"
     gene            complement(259..1344)
                     /locus_tag="PK442_09905"
     CDS             complement(259..1344)
                     /locus_tag="PK442_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HOO88009.1"
                     /translation="MTLRKGLVLFLLLSFGVSALVLASSVDADTWRTITNADGRLLLL
                     ALVFVGAAWCCDAARFCSLARAAGEKIGFRLGMVLTWLHYFGCAVTPMQSGGGPFQVY
                     VLYKKQVPLGKGIAITLTRTLLTLLILGLVVPVAVFIEPDLLKGKLFLKGVFSYVILF
                     IVVSWVLVLLSILRPLLIKKWGRVLTLWLKRFGFVKPETVLRVVRRINLEVDNYNLNF
                     RMFFSCGLKCFLVAILFSVLHLLCIFSVLPCLIAAMGLPFHYLQALLAQAVFMFLLYF
                     VPTPGASGVAEGGGAALFGLLVPWNMAGVTAIAWRFFTEYLAIGMGVIVAVRLLGWGV
                     TEEIFHKEPAGGDEPLIKEFDDEERRD"
     gene            complement(1561..>1993)
                     /locus_tag="PK442_09910"
     CDS             complement(1561..>1993)
                     /locus_tag="PK442_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88010.1"
                     /translation="GGVEARFETVPVRELVMDSLSEGTRYMSHLSAGLSKVADMLEAD
                     KTAEGLDLLRQAAEGIGWSLQVMHNCQILLGISDHEVGDGKVGELKTTLVQDLEKASS
                     SIENGKHLELSFRIRTGVLPGIQRMAQYMEALLETGRKAVQ"
BASE COUNT          562 a          559 c          488 g          384 t
ORIGIN      
        1 atgagccagt tgcctacgta gagagggttc cggacaattc cgtacggccc ccacgttgta
       61 agcctcgccg cgccgacatt ctctccccgg tatcgggtga tgcagcctgc agcccagaat
      121 cgcagcatct ggccaagagc gacaaggaca aggcccaaaa aagaacgata cggtcccggc
      181 ggctccgcta aaaaccagac ggcgagaaaa agcaccgtcc agataccgcc tcgcatcttg
      241 aaaacagcag cccggatact agtcacggcg ttcctcgtcg tcaaattctt tgattaacgg
      301 ttcatcgccg ccggccggct ccttatggaa aatctcttcg gtaacgcccc atccaagcaa
      361 gcgcaccgct acaatgacgc ccataccgat agcaagatac tctgtaaaaa atcgccatgc
      421 gatcgcggtc actccggcca tattccaggg gacaagaagg ccgaaaagcg ccgcaccgcc
      481 tccttccgca acgccgctgg cgccgggagt aggcacaaag tagagtaaaa acatgaagac
      541 cgcctgcgca agcaacgcct gaagatagtg gaaaggaagc cccatggcgg caatcagaca
      601 cgggagaacg gagaaaatac acagcagatg aagcacggaa aaaagtatcg caaccaggaa
      661 acactttagt ccgcaagaga aaaacatgcg gaagttgaga ttataattat ccacttccag
      721 attgattctt cgcacaacgc ggagaacggt ctccggtttg acgaatccaa agcgcttcag
      781 ccaaagcgtg agcactctcc cccatttttt tatcagcaga gggcgcagaa tgctcaggag
      841 caccaaaacc cacgatacaa caataaaaag aatcacatag gaaaaaacgc cttttaaaaa
      901 aagtttccct ttaagaagat caggctcgat aaagactgct actggaacta caagcccaag
      961 gatcaaaaga gtaagcagcg ttctggtcag agttatcgca atgcctttgc cgagaggcac
     1021 ctgttttttg taaagcacat acacctggaa agggccgccg ccgctttgca tcggcgttac
     1081 tgcacacccg aagtagtgca gccatgtaag caccattcca agtcgaaaac cgatcttctc
     1141 gcctgcggca cgcgcaaggg agcagaaacg ggcggcatca cagcaccagg cggctccgac
     1201 aaagacaagc gccagcaaaa gcaagcgccc gtcggcattt gttatcgtcc gccacgtatc
     1261 ggcatcgacg cttgaagcga gaacaagcgc gctgacgcca aaagaaagaa gcagaaacag
     1321 aacgagtcct ttacgaaggg tcaatacaga agtgcctcct cacaggttcc ggctgccccg
     1381 cagctctccg cgccagggcg gatataatgc ggaatcgggg gatagtgtag cagatttttt
     1441 cgtctagaaa agcgttctta aaaagaaaac gaccgctctc cggatcaaca ctcaaagaat
     1501 gttttccggc ccgttatcac ggagacgtca aaaaagttat gcctggcgtt ttgaaaagag
     1561 ttactgcact gcttttcggc cggtttccaa aagagcttcc atgtactgcg ccattctctg
     1621 aattccagga agcacgcctg tacggatacg aaacgaaagc tccagatgtt tcccgttttc
     1681 gatggatgag gatgcttttt ccaggtcctg tacaagggtc gttttgagct cgccgacctt
     1741 tccgtcgccc acttcgtggt cagagattcc cagaaggatt tgacagttat gcatcacctg
     1801 taaagaccat ccgatacctt cagcggcctg ccgtaaaagg tccagacctt cggctgtttt
     1861 atcggcttcc agcatatccg caaccttgga aagccctgcg gaaagatggc tcatgtaccg
     1921 agttccctcg gaaagcgaat ccatgacaag ttcccgtacc gggacggttt caaaccgggc
     1981 ttccactcct ccg
//
LOCUS       DAOSWW010000516         1992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47128_length_1992_cov_4.129582, whole genome shotgun sequence.
ACCESSION   DAOSWW010000516 DAOSWW010000000
VERSION     DAOSWW010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47128_length_1992_cov_4.129582"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..658
                     /locus_tag="PK442_09915"
     CDS             299..658
                     /locus_tag="PK442_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003156585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO88011.1"
                     /translation="MEKKRKFNCTIEASMCFIGGKYKPLILWHLIEGTKRFSALQRLL
                     PQATPRMLTMNLRELESDGLVHRKVYPEVPPKTEYSLSDLGKTLIPLITELREWGRYY
                     FEVTGQPNPCDVPSAEK"
     gene            complement(1224..>1992)
                     /locus_tag="PK442_09920"
     CDS             complement(1224..>1992)
                     /locus_tag="PK442_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO88012.1"
                     /translation="LFNLVGNGIKFTDSGEVTISLGCARRSGDETLLAVAVKDTGIGI
                     SPKGIERIFTPFSQADDSISRRYGGTGLGLAISKRLVEFMGGRIAVASKLGEGSVFSF
                     TLPVTVGAEDVAAETNSLNAGETPLRGLSVLVAEDNVFNRKVLEKILEKSGISFDSAV
                     NGVEAVKKGTEGAFDAILMDIEMPEMDGYEASRAIRRSEKETGRRVPIIALSAHVSEE
                     HRQMAFEAGMDAFASKPVRKTDLLRALAEATGREEAP"
BASE COUNT          403 a          579 c          550 g          460 t
ORIGIN      
        1 cgtcggcgaa ggcatccgca acataatact tcagcatcgt ttttcctcct tccaggtatt
       61 cgcacgtgat cgtttcactc cagaatcccc gatgagagcg gatcgccatg ccggtcgtac
      121 cggcatccgc gaagcgtctt gaaatactcg gagacacttg tcgcgagtgg agatatcggc
      181 ggctactcgt tcgccgtgtt tgatacgtct gtctttttac tataggataa tgatatggaa
      241 gacgcaagta cgcacatttc ttataggtac tatcaaaaac tatagcgggg gtatcctcat
      301 ggaaaaaaag agaaaattca actgtacgat cgaggcgtcg atgtgcttca tcggcgggaa
      361 atacaagccg ctgatcctgt ggcatctgat cgaggggacg aagcggtttt ccgctctgca
      421 gcggcttctt ccgcaagcga cgccccgtat gctgacgatg aatctgcggg aattggaatc
      481 ggacggtctc gttcaccgaa aggtataccc ggaggtgcct ccaaagaccg aatactctct
      541 ctcggatttg ggaaagactc tgatcccgtt gatcacggaa ttacgcgaat ggggaaggta
      601 ttatttcgag gttacgggac agccgaaccc gtgcgatgtg ccgagcgcag agaagtaaat
      661 ttccaaggtg tcgcggaatc ctttgcccgc tcgatctccg tattgattcc cttaaaagca
      721 cctttcatgc tcctcatagg gcgaatcggc acgagaacga ttttttcgtc gtacgacaga
      781 acctccagtc gagtccctgg ctttgcgccg atcgcgtctc cgatctcctt tgggattact
      841 atctggtact cgttggaaat agtgactgta ttcattagcc cgggcctccc ttcgtgagag
      901 ccgttttact tgtgattcag aataccctgc agaatatgcg tcgttctctg accatgcaag
      961 catagtatcg tttcggaaca aatgcaagag tatatgcgac gtgcccgggc gttattgcaa
     1021 accctgggcg gtgaaggcat gggtttgacg cgctctcggg cggctggctc tttttcggga
     1081 aggaaggccg ctcatggata tgcacgccgt cgtctggaag gctgttgatt ccatccgctc
     1141 gaaccgtgtc gaagaccttg acgtcgagac cgtcgccgag gcggtgaact ccacgaggtt
     1201 cccatttcga gggaggaacc tctctacggg gcttcctctc tccccgtcgc ctccgcgagg
     1261 gcgcgcagca ggtcggtctt tcgcaccggc ttacttgcga acgcgtccat ccccgcttca
     1321 aaagccattt gccgatgctc ttccgacacg tgcgccgaaa gcgcgatgat ggggacgcgg
     1381 cgtcctgttt ccttctcact gcgccggatg gcccgggagg cctcgtagcc gtccatctcc
     1441 ggcatctcga tgtccatcag gatggcgtcg aacgcgcctt ccgttccttt cttcaccgct
     1501 tcgacgccgt tcaccgcgga gtcgaacgag atccccgact tttccaggat cttctccagc
     1561 accttccggt tgaagacgtt gtcttcggct acgaggaccg aaagaccgcg tagcggcgtc
     1621 tcccctgcgt tgagggagtt cgtctccgcg gcaacgtctt ccgcgccgac cgttaccggc
     1681 agagtgaacg aaaagacgct cccctcgccg agcttcgaag cgacggcgat ccgccctccc
     1741 atgaactcga caagacgttt cgaaatagcc agtcccagcc ccgtcccgcc gtagcgcctt
     1801 gagatggagt cgtcggcctg gctgaagggc gtaaaaatcc tctcgatgcc cttcggagat
     1861 atgccgatgc cggtatcctt cacggcgacg gccagaagcg tctcgtctcc ggagcggcgc
     1921 gcgcacccga gggaaatggt cacctcgccg ctgtcggtga acttgatccc gttgccgacc
     1981 aggttgaaga gc
//
LOCUS       DAOSWW010000517         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47200_length_1990_cov_3.607752, whole genome shotgun sequence.
ACCESSION   DAOSWW010000517 DAOSWW010000000
VERSION     DAOSWW010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47200_length_1990_cov_3.607752"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PK442_09925"
     CDS             <1..1084
                     /locus_tag="PK442_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOO88013.1"
                     /translation="CRAEREKVKALGGLKILGTERHESRRIDNQLRGRAGRQGDPGSS
                     RFYLSLEDDLLRLFGSERIQGLMEKLGMEEGESIEHNLLTKAIANAQKKVEQLHFDIR
                     KQLLAYDNVMNRQREAVYTERQQILRDEGIQEHVWEIIEGVIDDILERYFPEEGEADP
                     ARAVARLKAIFGSGLEARLEGVDNVRDMLAHRDELVAEIRDRFIGKVETLGKEEGRAL
                     LRFLVLHTLDSGWKDHLLAMDELRRGIGLRAIGQKDPLLEYQFESYNLFQSMMERVRE
                     SVAELAFRVRVVSERGGASDGSNRREGREFILPFGASQEQQPPSLKHGVEGVQQPVKK
                     GPKTGRNDPCPCGSGKKFKHCCGKNA"
     gene            1233..>1990
                     /locus_tag="PK442_09930"
     CDS             1233..>1990
                     /locus_tag="PK442_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88014.1"
                     /translation="MIQECPSFSDYPGSSGVIWLKDVRYSLGADGAMTRDSVFVILAR
                     RGIDESWSRWTLPVPEDGGSVEVFSAALYDPGSGRMLAPVLPRKSQSGGVSVVELAFP
                     DLQEEYIISLSYREVFPKRFAVDDFIWVNETLPLWELQVTVDVPAGSDLAVSARGVGD
                     LRRENAGAVERHAWHMVNNLPWTTRTLRGDARSYLAFSTRKGLEPLARMLSAYENVLV
                     PQPSAPVEKLINQQNKLRTGSALISWMNAAPSYQG"
BASE COUNT          441 a          500 c          607 g          442 t
ORIGIN      
        1 gtgccgcgcg gaacgcgaaa aggtaaaggc tctcggaggt ctcaaaatac tggggactga
       61 gcgtcatgaa tccaggcgga tcgataatca gcttcgaggt cgcgcgggac gtcagggcga
      121 tcctggctcc agccgcttct atctttcgct tgaagacgat cttctccgtc ttttcggctc
      181 cgaacgtatt caggggctca tggagaagct cggaatggag gagggagagt cgatagagca
      241 caatctcctc accaaagcga ttgcaaatgc tcagaaaaaa gtggaacagc tccatttcga
      301 tattcgaaaa cagctcttgg cgtatgacaa cgtgatgaac cgccagagag aagccgtcta
      361 taccgaacgc cagcagatcc ttcgcgacga ggggatccag gagcatgtct gggaaattat
      421 cgaaggtgtg atcgacgata tccttgagcg gtacttcccc gaggaagggg aagcggatcc
      481 ggcacgcgcg gttgcccgtc tgaaggctat cttcggaagc gggcttgagg cccggctgga
      541 aggtgttgat aacgtccgcg acatgctcgc gcaccgtgat gaactggtgg cggaaatcag
      601 ggaccggttc atcgggaaag tggagaccct cggtaaagag gaggggcggg cgctgcttcg
      661 tttccttgtg cttcatacgc tcgacagcgg ctggaaggac catctccttg caatggacga
      721 actgcggagg ggaatcggcc ttcgtgcgat cggacagaag gacccgttat tggaatacca
      781 gtttgaatcg tacaacctct ttcagtcgat gatggagcgc gtgcgggaat ctgttgccga
      841 actggccttc agggttcgcg tagtgagcga acgcggcggt gcgagcgacg gaagtaaccg
      901 ccgcgaagga agagagttta ttcttccttt cggcgcctcg caggaacagc agccgccttc
      961 actgaaacac ggagttgagg gcgtacagca gcctgtcaaa aagggaccga agacagggag
     1021 gaacgacccc tgtccatgcg gcagcgggaa aaaattcaag cactgctgcg gtaaaaatgc
     1081 ataacaaggg cgtctggatg cggaaaggag agtgtgcaat gaacgcgtgc aaaaaaatgt
     1141 tttcaggtgt tttgtctcta cttgccgttg gtgtagttct cttttattca cccggagaga
     1201 gtttttccgc gctttcggcg aacattcccc gtatgatcca ggaatgtccg agtttttccg
     1261 actacccggg aagctccggc gttatctggc tgaaagatgt ccggtattct ttaggcgccg
     1321 acggggctat gacccgcgat tcggtcttcg ttattcttgc ccggcgaggg attgacgaat
     1381 cgtggagcag atggacgctt cctgtacctg aggacggagg gagcgtggag gtcttttctg
     1441 cagctcttta cgatcccggc tccgggcgga tgctcgctcc cgttcttccc agaaagagcc
     1501 agtccggcgg ggtttcggtt gtcgagctcg cttttccgga tcttcaggaa gagtacatta
     1561 tctctctttc ctaccgggag gtctttccaa aacgctttgc cgtagatgat ttcatttggg
     1621 tgaatgaaac cctcccgctc tgggagcttc aggttaccgt cgacgttcct gccggcagcg
     1681 atcttgcagt ctcggcgaga ggcgtcggcg acctgaggag agaaaatgcc ggcgctgtgg
     1741 aacgccatgc ctggcatatg gtgaacaatc ttccgtggac gacgcggacg ctgcgaggag
     1801 atgctcggtc ctatctggct ttttccacca gaaagggact tgaacctctt gcgagaatgc
     1861 tttcagccta cgagaatgta ctcgttccgc agccttcggc tcctgtggaa aaactgatca
     1921 atcagcagaa caagctgcga acggggagcg cgcttatttc atggatgaat gccgcgcctt
     1981 cctaccaggg
//
LOCUS       DAOSWW010000518         1988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47300_length_1988_cov_2.736679, whole genome shotgun sequence.
ACCESSION   DAOSWW010000518 DAOSWW010000000
VERSION     DAOSWW010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47300_length_1988_cov_2.736679"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1317
                     /locus_tag="PK442_09935"
     CDS             151..1317
                     /locus_tag="PK442_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOO88015.1"
                     /translation="MSDRKKFFWIAAFFLTFYFIPEGNERVLAGLAESVAMMADYARE
                     HVLLCLIPAFFIAGAISVFVSQQSVMKYLGAKAKKWIAYSVASVSGTVLAVCSCTVLP
                     LFAGIYTRGAGLGPATAFLYSGPAINVLAIVLTARVLGLELGVARAVGAVLFSVIIGL
                     LMAAIFGKEEKARQEGFAQIPEDEGGRPLWKTVVYMASMVVFLVFANWAAPKTSNAFW
                     EGVYNLKWILAGAGIAVTLLTSWLWFTRDERQDWYTSTWGYALQVMPLLFGGVLVAGF
                     LLGRPGHEALIPSRYVAMLVGGNSLAANFFASISGAFMYFATLTEVPILQGLLGAGMG
                     KGPALALLLAGPALSLPSMLVINSILGLRKTATYVVLVVALSTVAGMLFGVLAS"
     gene            complement(1333..>1988)
                     /locus_tag="PK442_09940"
     CDS             complement(1333..>1988)
                     /locus_tag="PK442_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88016.1"
                     /translation="IEIPLSLLAAVAILVMGNDIFIDNAAWSGLSRIDGIVLLMFMII
                     FLSYNVTLARAGNLDADTDIKEYPPRKALLMLLAGLLLLSGGGRAIVFFATRFARDLG
                     ISERVIALTVISIGTSLPELATSVVAAMKKRVDIAIGNVVGSNLFNTFFVLGSSAIIA
                     PVTVPALSNLDMGLNIAASALLFIFVFTGKGRAIERWEGAIFCALYVSYLASLVFWG"
BASE COUNT          356 a          572 c          652 g          408 t
ORIGIN      
        1 tccttcgttc gagaaggtga aggagctgct tctgaaggcg aaggggtaag ccgatacacc
       61 atgcccccgc tcccggcggg ggtttcccct tgctctttaa gtgaataaaa tgccacataa
      121 tggcgatact gtatgaaatg gagagagaca atgagcgatc gtaaaaagtt tttttggata
      181 gccgcgtttt ttctgacgtt ctacttcatc cctgagggga acgagcgcgt tctcgcagga
      241 cttgcggagt ccgtggccat gatggctgac tacgcgcggg agcacgtgct gctctgcctg
      301 attcccgcgt tcttcatcgc cggggccatc tcagttttcg tgagtcaaca gtcggtgatg
      361 aagtacctgg gtgcaaaggc gaagaagtgg atcgcgtact cggtggcatc ggtttccggc
      421 acggtgctgg cggtctgctc ctgcacggtg ctgccgcttt tcgcggggat ctacacccgg
      481 ggcgcggggc tcggacccgc cacggctttc ctctactccg gtccagctat caatgtgctc
      541 gctatcgtgc tgacggcgcg ggtgctcggg cttgaactcg gcgttgcacg cgcagtgggc
      601 gccgtgctct tcagcgtgat catcggcctg ttgatggccg ccatcttcgg caaggaggag
      661 aaggcccggc aggaggggtt cgcgcagatt cccgaggatg agggcgggcg tccgttgtgg
      721 aagaccgtcg tctacatggc gagcatggtc gtctttctcg tctttgccaa ctgggccgcg
      781 cccaagacgt ccaacgcctt ctgggagggc gtgtacaacc tgaagtggat tctcgccgga
      841 gcggggatcg ccgttacgct ccttacctcg tggctgtggt tcacccgcga cgagcggcag
      901 gactggtaca cgtcgacatg ggggtacgcg ctccaggtca tgccgctgct cttcggcggc
      961 gtgctcgtcg cggggttcct cctgggacgg ccggggcacg aggcgctcat cccgagccgg
     1021 tacgtcgcca tgctggtggg cggcaactcg ttagcggcga acttcttcgc gtccatctcc
     1081 ggcgcgttca tgtacttcgc cacgctgacc gaggtcccca ttcttcaggg gctgctcggc
     1141 gcgggtatgg gaaaaggccc cgcgctcgct ctgctgcttg cggggcccgc gctttcgttg
     1201 ccgagcatgc tggtgatcaa cagcatcctc ggcctcagga agaccgcgac ctatgtcgtg
     1261 ctcgtggtgg cgctctccac ggttgccggg atgctcttcg gggtgcttgc ttcctgagaa
     1321 agagctttcc cgctaccccc agaacaccag cgacgcgagg tacgatacgt agagggcgca
     1381 gaagatcgcg ccctcccatc gttcgatggc cctgcccttt ccggtaaaga caaagatgaa
     1441 gaggagagcg cttgccgcaa tgttgagccc catatcgagg ttcgacagag cgggaaccgt
     1501 caccggggcg atgatcgccg aagagcccag aacgaagaag gtattgaaga ggttcgaccc
     1561 cacgacgttg ccgatcgcga tgtcgacccg cttcttcatc gcggcgacaa cggacgtggc
     1621 gagctccggc agcgacgtcc cgatggagat gacggtgagg gcgatgacgc gctcggagat
     1681 gccgaggtct cgggcgaagc gcgtggcgaa gaagacgatg gccctgcctc ctccgctcag
     1741 caggagaagt cccgcaagga gcatcagaag cgctttccgg ggaggatact cctttatgtc
     1801 ggtgtcggcg tccagatttc ccgccctggc gagcgttacg ttgtacgaga gaaagatgat
     1861 catgaacatc agaagcacga taccgtctat cctcgagagg ccggaccatg ccgcgttgtc
     1921 gatgaagatg tcgttgccca taacgaggat ggcgacggcg gcgagcaggg agagcgggat
     1981 ttctatcc
//
LOCUS       DAOSWW010000519         1985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47393_length_1985_cov_3.664767, whole genome shotgun sequence.
ACCESSION   DAOSWW010000519 DAOSWW010000000
VERSION     DAOSWW010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47393_length_1985_cov_3.664767"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PK442_09945"
     CDS             complement(<1..406)
                     /locus_tag="PK442_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOO88017.1"
                     /translation="MKKLKECTVSVFGLGLLGASLAWKLTSGGSVQGVAGWSRTRETV
                     QRALDAGMITHACDSAEECAALGDMLILAVPIRSMEETSKSIAGAVRPDALAVFDLAS
                     TKTEVGRSLSSIWGPRYAGFHPMAGKEKGGIGN"
     gene            complement(408..866)
                     /locus_tag="PK442_09950"
     CDS             complement(408..866)
                     /locus_tag="PK442_09950"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOO88018.1"
                     /translation="MNLWSLLGMMFVLLAFIGALLPVMPTVPFVLVAAACFAKSSPKM
                     HAWLQQHPKFGHIVCDWEEKRCISRGMKIWGISMMTLGGGISVWLVVPRGWPSWTVLG
                     CFIAADMVVLLWKECPSEPGSTRRLMAEGLRKLFFFRGRNGKNSTGKECE"
     gene            complement(1021..1914)
                     /locus_tag="PK442_09955"
     CDS             complement(1021..1914)
                     /locus_tag="PK442_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOO88019.1"
                     /translation="MKEATRAAYEESILRVQMHMQKTLDDPFDANEIASVAGFSRFHL
                     SRLFSAMTGESLLEYRRRLLLERAAHSLLYKVRSVTELAFEAGYETPETFTRAFSKLY
                     GVPPGEYRRKLRAAMKHRLEMIQVRSKLFTLSLSIDEGGRKNMEVKIEKHDPLKVAFI
                     RHTGAYDTCGEAWGKLCGAPEVASKLGPDTLALSLCYDDPDVTEPDKIRMDVCVTVDD
                     GFVPGNGIEVQTIPGGEFAVVVYKGAYSGLHDAYRAIYGEWMPKSGREFGDSYSMEIY
                     RTDPEKTPPEESITEIRIPLL"
BASE COUNT          418 a          642 c          531 g          394 t
ORIGIN      
        1 cgttaccgat cccccccttc tccttccccg ccatggggtg aaaccccgcg tatcgcggtc
       61 cccagataga tgataacgaa cgccccacct cggtcttcgt actggcgaga tcaaagacag
      121 cgagcgcgtc agggcgcacc gcgcccgcga tactcttgct cgtctcctcc atcgaacgga
      181 tgggcactgc gagtatcagc atatcgccaa gcgccgcgca ctcctcggca gaatcgcagg
      241 catgcgtgat catacctgcg tcgagagcac gctgcacggt ttcccgagtg cggctccaac
      301 cggcgacgcc ttgcacgctc ccgccggacg tcaacttcca tgcgagcgac gctcccagca
      361 gtccgagtcc gaagacggag acggtgcact cctttagctt cttcatgcta ctcgcactcc
      421 tttcccgtac tgtttttccc gttccttccc ctaaaaaaga acagtttccg caacccctcc
      481 gccatgaggc gcctcgtgga tccaggttcc gagggacact ctttccacag gagaacgacc
      541 atatcggcag cgatgaagca gccaagaacc gtccaggacg gccatccacg gggcacgaca
      601 agccacacgg agattccgcc gccaagggtc atcatactga ttccccagat cttcatcccc
      661 ctggagatgc agcgcttctc ctcccagtcg cacactatat ggccgaattt cggatgctgc
      721 tggagccatg cgtgcatttt cggggaggac ttcgcgaaac aggccgccgc gaccagaaca
      781 aaaggaacag tcggcatcac gggaagcaga gcgccgataa acgcaaggag aacgaacatc
      841 attccgagca gagaccagag attcattgca caccaccagt tcaaattgat tacgggacgg
      901 ggcaacgata gcacggaata gaaaaaaacg ggagaggaga agcacggaaa aaatccggac
      961 ggaccacccg cggggcgcga gcggtctgtc cggcgatttg tgatcagttt tcgcgaggcg
     1021 ttacagcaac ggtatcctga tctccgtgat actttcctcc ggcggcgttt tctcgggatc
     1081 cgtccgatag atctccatcg agtacgagtc tccgaactcg cgcccgcttt tcggcatcca
     1141 ttcgccgtag atggcgcggt acgcgtcgtg cagacccgag tacgccccct tatagaccac
     1201 gacggcgaac tcccctccgg ggatggtctg cacttctatt ccgttgccgg gaacaaaacc
     1261 gtcgtcaacg gtgacgcaga catccatccg gatcttatcg ggctccgtca catccggatc
     1321 gtcgtagcag agcgagaggg caagagtatc cggacccagc ttggacgcga cttcaggcgc
     1381 cccgcaaagt ttgccccacg cttcgccgca cgtgtcgtac gcccccgtgt gccggatgaa
     1441 cgcaactttc aaaggatcgt gcttctcaat ttttacttcc atattttttc ttcctccttc
     1501 gtcgatggag agggaaagtg tgaagagctt cgacctgacc tggatcatct ccaggcggtg
     1561 cttcattgcc gcccgcagct ttcgccggta ctcccccggg gggacgccgt agagcttgga
     1621 gaacgccctt gtgaatgtct ccggcgtttc gtatccggct tcgaaggcaa gctccgtcac
     1681 cgaacgcacc ttgtagagca ggctatgcgc cgcccgttcc agcagcagcc gcctccggta
     1741 ctccagcagg gactcgccgg tcatcgccga gaagaggcgc gagagatgaa acctggagaa
     1801 tccggcgacc gaggcgatct cattcgcatc gaacggatcg tccagcgttt tctgcatgtg
     1861 catctgtacc cggagaatcg actcttcgta ggccgcgcgc gtggcttctt tcattcttcc
     1921 ccctccttgt gacacagagg ataccgcatc cggaacgcaa ccgcctgacc gtcgttgcgc
     1981 atttt
//
LOCUS       DAOSWW010000520         1982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47498_length_1982_cov_3.276596, whole genome shotgun sequence.
ACCESSION   DAOSWW010000520 DAOSWW010000000
VERSION     DAOSWW010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47498_length_1982_cov_3.276596"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..829
                     /locus_tag="PK442_09960"
     CDS             167..829
                     /locus_tag="PK442_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtE intracellular region"
                     /protein_id="HOO88020.1"
                     /translation="MPEEEMELERAEGEVVPPPKKKKKKTKGSKLPFFLLVLLMAAGV
                     GAGLHIAGAWDARPLLYASVPKIPWIGNDLAKLTGIPEEYTLTVEQRRRLELQRWNDR
                     LDELERSLKERETALEALSADLEARSISVEKKEAEFTLKENKPPESPEEEKAYVDMLM
                     RTYQEISPRRAALIIEQLREDLAVKMLAAMPQDARASILGRMEAARAARLTERLAAGG
                     SR"
     gene            complement(1503..>1982)
                     /locus_tag="PK442_09965"
     CDS             complement(1503..>1982)
                     /locus_tag="PK442_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOO88021.1"
                     /translation="AYLQGKNIRERAMDLISIAHPDFRPWLIEEAKRLKLIYKDQAYI
                     PGKCGEYPESLAIHRTTRSSQEILLRPVKISDEPLIKDFFYSLSDESLYTRFISTRRN
                     VPHEMRQRGYQRPPGLFPAEELLLPGLFFLPQSPEDVQALPVSQDRLRFSDQPPSQP"
BASE COUNT          463 a          540 c          604 g          375 t
ORIGIN      
        1 aactggccga taagtatacc ggggacctta cgaaaacgct tgccgcgtac aacgctggac
       61 ccggaagggt tgacgcctac gggggaattc cccccttcgc cgagacgcag aagtatgtac
      121 agagcattct cgctctctac gaacgcaatt ctgaaggaga tagctgatgc ccgaagaaga
      181 gatggagctc gagagagcgg agggcgaggt tgtccctcca ccaaagaaga aaaaaaagaa
      241 aacaaaagga agtaagctgc cgtttttcct cctggtgctc ttaatggcgg ccggagtcgg
      301 cgccgggttg catatcgccg gagcgtggga tgcacggcct cttttgtacg cgagcgttcc
      361 gaagatcccg tggataggaa acgatctcgc caagctgacc ggcattccgg aagaatacac
      421 gcttaccgtc gagcagcggc gcaggcttga acttcagcga tggaacgacc ggctcgacga
      481 actggagcgc agcctcaaag aacgggagac agccctcgaa gcgctttccg ccgacctcga
      541 agcacgaagt atttcggtcg agaagaagga ggcggaattc actttaaagg agaacaagcc
      601 tccggagagt cctgaagagg aaaaagcgta cgtcgacatg ctgatgcgga cgtaccagga
      661 aatatcgccg agacgggcgg cgctgatcat cgagcagttg cgcgaggatc tggcggtaaa
      721 gatgctcgcc gcgatgccac aggacgcgag agcgtccatt ttgggccgca tggaagcggc
      781 cagggcggca aggctgacgg agcggctggc ggccggaggg tcgcgataac gattcgggag
      841 gtgcggcttc atgttttcag ccttgtttcc agtgcataaa gatggaatga ccgacgtcct
      901 tccaggcgcc gagacgccgg gaagcgtaaa gaacggaggc gacggcgaaa aaaaggagca
      961 gttcgccaaa ctgctctttg tttcgttgga gactgcggac gacggtaaaa atacgaccac
     1021 ggaaaccccc ggggaaaccc ctttgtggac aaacggcgcc cgttcgttga ccgcacttgc
     1081 cgcctctctt ttaggagaga atgcaggcca ggaagcagcc gttattgcgg atgcggccga
     1141 tgaagatcct tctgcacaga tcacccccga cgattcgctc acaagcctgt ttgcttccgc
     1201 atttgccgat gcaaaagacg ctacggcgca aatcccggcg gcttccgaaa ccccaacgaa
     1261 tgaggtttta tccgaaacgg aagcggtcga aatgcaccag aggcaggcag ccggtgacct
     1321 tgagttttcc tccccgaacg ctcggcttct tgacgaggcg cttcttacca aggagacgct
     1381 tcccgtcgat caaactcttc caactgatga tgtttcgaaa ggatcgctca aacaggctgc
     1441 gtccccggag ttaacccttc cccatggtga gggaaagggc gcttttgccc tgccggattc
     1501 ctttaaggct gtgacggcgg ttgatccgaa aagcgcagtc gatcctgaga tacaggaagc
     1561 gcttggacgt cttcaggcga ctgcggaagg aaaaacagcc ccggcaaaag aagctcttca
     1621 gccggaaaga gcccaggcgg gcgctgatat ccgcgctgcc tcatctcgtg ggggacgttc
     1681 ctcctcgtcg agatgaacct cgtgtagagg ctctcgtctg agagggagta gaagaagtcc
     1741 ttgatcaacg gctcgtcgct gatcttgacg ggccgcagca ggatctcttg ggaggagcgg
     1801 gtcgtcctgt ggatcgccag gctctcggga tactcgccgc actttccggg gatataggcc
     1861 tggtccttgt atatcaactt cagcctcttc gcctcctcga tcagccaggg ccggaagtcc
     1921 gggtgggcga ttgaaatcag gtccatcgcc ctctcccgga tgttctttcc ctggaggtag
     1981 gc
//
LOCUS       DAOSWW010000521         1978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47623_length_1978_cov_14.508580, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000521 DAOSWW010000000
VERSION     DAOSWW010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47623_length_1978_cov_14.508580"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1057)
                     /locus_tag="PK442_09970"
     CDS             complement(5..1057)
                     /locus_tag="PK442_09970"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOO88022.1"
                     /translation="MDEAHRNGARLFMACRELGIDIRTRQRWLKDAAEGGVNTDGRKD
                     AERKKPVGALSEEERERVLEIADSPEYASKAPAQIVATLADKGEWVASESTFYRILHE
                     KNQQHNRGKAKSPNRKTPTSHAATAPNQVWTWDITYMGSPVRGKYYYLYMIVDIFSRY
                     IVGWEIHEEENAQYAKTLVQKAYLKEAIWKNGTPLVLHSDNGSPMKASTFRATLERLG
                     ITGSYSRPRVSDDNPYSESLFRTLKYRPEYPTYGFESLEASREWTYGFVHWYNEIHRH
                     SALKYVTPAQRHRGESAAILKARKSVLEEAKRKHPERWKGRAVRNLEEAQTVWLNPEK
                     DGRARETLTPEKRQVG"
     gene            complement(1093..1590)
                     /locus_tag="PK442_09975"
     CDS             complement(1093..1590)
                     /locus_tag="PK442_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOO88023.1"
                     /translation="MKRYSPEFKESVVRKMMPPENVPIMRLSEESGVSYVTLAKWRDE
                     VRGGGEAAPGNGERPDRWSSEDKFLIVLETHSLNEAELGEYCRSKGIFPAEIEEWRET
                     CLMANQRDLRNRQEMQKEIRAEKKRSKELERELLRKERALAETAALLTLRKKAQAIWG
                     DDEED"
     gene            complement(1617..>1978)
                     /locus_tag="PK442_09980"
     CDS             complement(1617..>1978)
                     /locus_tag="PK442_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88024.1"
                     /translation="VMSFCENVSVVQGFTAEQNALLYAVQSLSPKDPGAQLNLGLLRA
                     LELAKIKSSGLPARRMILLCSDGFRETPGGATADEVRNALASGPVPVYSIFFDSPERE
                     SPLCHNRSRLNRGVKEV"
BASE COUNT          298 a          639 c          543 g          498 t
ORIGIN      
        1 tctgtcaacc gacctgtcgc ttttccgggg ttaaagtttc ccgcgctctt ccatccttct
       61 ccggattgag ccataccgtt tgtgcctctt ccaggtttct caccgcacgc cccttccacc
      121 gctccgggtg cttcctcttc gcttcctcca gaactgattt cctcgccttg agaatcgcgg
      181 ccgattctcc acggtgacgc tgggcgggcg tgacgtactt caacgcgctg tgacggtgga
      241 tctcgttgta ccaatggacg aagccgtacg tccattctct cgacgcttca agactctcga
      301 atccgtaggt cgggtattcg ggcctgtatt tgagcgtccg gaagagggac tcggagtacg
      361 ggttgtcgtc gctgactctc gggcggctgt acgaaccggt tatgccgagt ctttccagcg
      421 tggctctgaa cgtggaggcc ttcatggggc ttccgttgtc cgagtggagc accagcggcg
      481 tcccgttctt ccagatcgct tccttaagat acgctttctg taccagcgtc ttcgcgtact
      541 gcgcgttctc ctcctcgtga atctcccacc cgacgatgta gcggctgaag atgtccacga
      601 tcatgtagag gtagtagtat ttgccacgga ccggcgagcc catatacgtg atatcccaag
      661 tccacacctg gttcggggct gtcgccgcat gcgacgtcgg agtcttcctg ttcggactct
      721 tcgccttgcc tctgttgtgc tgctggttct tctcatggag tatccggtag aacgtcgatt
      781 ccgacgccac ccattcccct ttgtccgcaa gcgtcgccac tatctgcgcc ggagccttgc
      841 tcgcgtactc ggggctgtcg gcgatctcga gcactctttc ccgctcctct tcgctcaagg
      901 ctcctacggg ctttttgcgc tccgcatcct ttcttccgtc cgtgttcacg ccgccttcgg
      961 ccgcgtcctt gagccatctc tgacgggtgc ggatgtcgat tccaagctcc cggcaggcca
     1021 tgaagaggcg tgctccgttt cgatgcgctt cgtcgatcag ctctaccgct ctcaggcgat
     1081 ccggggcgct gatcagtctt cctcgtcgtc cccccagatc gcctgcgctt tttttcgcag
     1141 cgtcagcagc gccgccgtct cggccagcgc cctctccttc cgcagcagct ctctttcaag
     1201 ttccttgctg cgcttcttct cggcccggat ctctttttgc atctcctggc gattgcggag
     1261 atcccgttgg ttggccatca ggcacgtctc gcgccactct tctatctccg cggggaagat
     1321 tcccttggac ctgcagtatt cgcccagttc ggcctcgttg agagagtgcg tctccagaac
     1381 gatcaaaaac ttgtcctcgg aactccaacg gtcggggcgt tcgccgtttc cgggcgcggc
     1441 ttcgccgccg ccccgcactt cgtctctcca cttcgccaac gtcacgtacg agacgccgct
     1501 ctcttcggag agtctcatta tcggtacgtt ctccggcggc atcatcttcc gcacgacgga
     1561 ctccttaaat tcagggctgt atctcttcat cctcatcgca cctcccgagt gtcgactcac
     1621 acttctttaa cacctcggtt caagcgactt ctattatgac acagggggct ttcgcgttcg
     1681 ggcgagtcaa agaaaatgga gtacaccgga acagggccgg aggcgagcgc gttgcggact
     1741 tcgtcggctg tggcgcctcc cggcgtctcg cggaagccgt cgctgcacag gaggatcatc
     1801 cggcgcgcag ggagtccgga gctttttatc ttcgccagtt cgagggcgcg gagaagaccg
     1861 agattgagct gcgcgcctgg gtctttgggt gaaagagact ggacggcgta cagcagtgcg
     1921 ttctgttcgg cggtgaagcc ttggacgacg gagacgttct cgcagaacga catcacgg
//
LOCUS       DAOSWW010000522         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47720_length_1976_cov_3.364914, whole genome shotgun sequence.
ACCESSION   DAOSWW010000522 DAOSWW010000000
VERSION     DAOSWW010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47720_length_1976_cov_3.364914"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PK442_09985"
     CDS             complement(<1..802)
                     /locus_tag="PK442_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO88025.1"
                     /translation="MLEKIKKLATAYGDDVVAFRREFHMHPERSWEEVETSKKVVEAL
                     KGLGCTIVKTGFGGTESGVVAEMKGGKPGKCVALRADIDALPLQEENKVPYASQTPCV
                     MHACGHDAHTAILLGAAKILAEIRDELPGTVRFIFQPAEECGLRSGAKVMLDEGALDG
                     VDSIGGLHVWSLAPTGTALYRSGPVMAAADAWYLTIKGKGGHGSAPENAIDPTITAAN
                     LVLNLQAIVGREVSARETAVVSTGVVKAGETVFNIIPDSVTMNGTVRTF"
     gene            1180..1575
                     /gene="rbsD"
                     /locus_tag="PK442_09990"
     CDS             1180..1575
                     /gene="rbsD"
                     /locus_tag="PK442_09990"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HOO88026.1"
                     /translation="MKRSGILHPELAFQIASCGHGDLVCIADAGLPIPPETPRIDLAY
                     ALGQPAFFNVLEAILPELAIEGAVWAEEALAKSPMLAGRLETLFQQTPHERIPHEEFK
                     KLLPDVRFVVRTGECTPYANVLLRCGVPF"
     gene            1591..>1976
                     /locus_tag="PK442_09995"
     CDS             1591..>1976
                     /locus_tag="PK442_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88027.1"
                     /translation="MPLFRRLDSRTLLLLLLGGLMFVAGSLEPRFFTGANLLNVLRQS
                     SIIGVISLGMTVVILTGGIDLSVGSVLSLSVLFAASASKTGLPAPVAWACALLAGAGL
                     GWVNGFGVSRLRLPPFIVTLGMMGFAR"
BASE COUNT          347 a          676 c          573 g          380 t
ORIGIN      
        1 tgaatgtgcg cacggtgccg ttcatcgtca cggagtcggg aatgatgttg aagaccgtct
       61 cccccgcctt gacgacgcct gtgctcacta ctgccgtctc gcgcgcgctc acctcgcggc
      121 cgacgatggc ctgaaggttg agcacgaggt tcgccgccgt gatcgtcgga tcgatggcgt
      181 tctccggagc ggaaccgtgc ccgcccttgc ccttgatcgt caggtaccac gcgtccgcag
      241 ccgccatcac agggccggag cggtagagcg ccgttcccgt gggggccagc gaccatacgt
      301 ggaggccgcc tatggagtcg acgccgtcga gcgcgccctc gtcgagcata actttcgccc
      361 ccgaccgcag accgcactcc tcggcgggct ggaagatgaa acggacggtt ccgggaagct
      421 cgtcacggat ctcggcgagg atcttcgccg cacccagcag gatggcggtg tgcgcgtcgt
      481 ggccgcaggc gtgcatcacg cacggagtct gcgacgcata cgggaccttg ttctcctcct
      541 gaagcgggag cgcgtcgatg tcggcgcgca gcgccacgca cttgcccggc ttgccgccct
      601 tcatctcggc gacgaccccc gactccgtgc cgccgaaccc cgtcttcacg atggtgcagc
      661 caagcccttt cagcgcctcg acgacctttt tgctggtctc gacctcttcc caggagcgct
      721 ccggatgcat gtggaactcg cgccggaatg cgacgacatc gtcgccgtac gccgttgcca
      781 gtttcttgat tttttcaagc aaaagaaatc acctcgttca atcgaatttt agaatacgaa
      841 acagatatgc aaaaacacta tcattctttt ccgcaccgca caagcccccc tcgaaacaga
      901 tagctgaaaa acatccgggg tgtcgcgcag cggcatcttt ccgcacgccc gcaccttccg
      961 cagatcacag tctgaagtag gtacaatgca cgaatttctc tggtcgaaga cgcgtgattc
     1021 acgttgtaaa aaccatgcct ccgtgctacg atacatgaca atagcatgat aaattacagc
     1081 gcttctcttg agtgaacgac gggcgggcgc aaggccacgg aagtatgcga tagacggccc
     1141 gcacgcttca ccatgagcga acgcaagggg ggacgcacta tgaaacgcag cggcattctt
     1201 cacccggaac tggctttcca gatcgcttcc tgcggccacg gcgaccttgt gtgcatcgcc
     1261 gacgcggggc ttccaatacc cccggagacg ccacggatcg acctggccta cgcgctcggg
     1321 caaccggcct tcttcaacgt gctggaagcc atccttcccg aactcgcaat cgaaggcgcc
     1381 gtctgggccg aagaggcgct cgccaaaagt ccgatgctcg ccggacggct ggaaacgctc
     1441 ttccagcaga ccccgcacga acgcattccg cacgaagagt tcaagaagct cctgcccgac
     1501 gtccgcttcg tcgtccgcac gggcgagtgc acaccgtacg ccaacgtgct gctccgctgc
     1561 ggcgttccgt tctaaagaag gcggaaggcc gtgccgctct ttcggcgtct cgactcgcga
     1621 acgctgctcc tgctgcttct gggggggctc atgtttgttg cgggctcgct ggagccgcgc
     1681 tttttcaccg gcgccaacct gctgaacgtg ctgcgccagt cctccatcat cggcgtcatc
     1741 tcgctgggca tgaccgtggt cattctcacc ggaggcatcg acctctccgt cggcagcgtc
     1801 ctctcgctct ccgtcctctt cgccgcctcc gcgtcgaaaa ccggcctccc cgcccccgtc
     1861 gcgtgggcgt gcgccctcct cgcgggagcc gggctgggct gggtcaacgg gttcggcgtc
     1921 tctcggctgc gcctcccccc cttcatcgtc acgctgggaa tgatggggtt cgcgcg
//
LOCUS       DAOSWW010000523         1975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47780_length_1975_cov_2.025521, whole genome shotgun sequence.
ACCESSION   DAOSWW010000523 DAOSWW010000000
VERSION     DAOSWW010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47780_length_1975_cov_2.025521"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(761..1321)
                     /locus_tag="PK442_10000"
     CDS             complement(761..1321)
                     /locus_tag="PK442_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88028.1"
                     /translation="MKLAGKLLTVAERKETWLKRVLQGGKSFRESEPGKRCELWIGGV
                     WAAVRGRDYESGKRWSYREVVSAIQDIRKRRLVFSLVYTSVLAGTLTFLKLQGVQSDE
                     SASKILRTLFSIAPVVFALYIKILVARSYHYLSGADTEIRAVLSQPLSASKSLFDATL
                     VFLLWNPVMDIRSLEHFGEILLSLIR"
BASE COUNT          490 a          490 c          496 g          499 t
ORIGIN      
        1 ttattcgggt cgtgcggaga ggtggccgag tggtcgaagg cgattgactc gaaatgaatg
       61 tataaaataa aagcaaatat tatactcaaa acacgttaat gcttagcaca ttccgctatg
      121 acaaaaatta tatcacaaaa aataggaata tcaagtgatt gattgaattt tttattgagt
      181 taacttttaa atagattgga atataattgt tatctttaac tgtaggccgc gttgtttgag
      241 cggtcttttt gtttgggttt tcttgatcct ttcgaacctg ccttctaaat cacttttcat
      301 atgtatcatc gtttccccaa atcagagaac atccgttgtc gtgtcgaatt cgaggtttgt
      361 gaaagtctgc ttttgctact gcgtctagaa tgctctcacc cgagcgaata tacacacggg
      421 ccgagaaaga gcaggggaat accacagcag gcaagagaat tccctaaagc gaaaatttgc
      481 cagaagatgt gaatccttcc tgattcggat cctcgaatct tcgctcgcgg tctttcctgg
      541 attaaaatac aacgcatatt ttctcttctg gctaaggaag tcacccccta ctttttctcc
      601 gcgtatcttt ccctcggtat tctctagcag ctaaaaaaca tgagccgagg aacggttgct
      661 caaaacatct catcgatggc atgcactgat gctctcgcct tttgagagaa ttcctcgtcc
      721 agcttgcagg aatgctcgac gcgaaggggg caaagggcga ctagcggatc aacgagagca
      781 agatctctcc gaaatgctcc agtgaacgga tgtccataac gggattccaa agtagaaaga
      841 caagcgtcgc gtcaaacaga ctcttgctcg ccgagagcgg ctgagagagc acggctcgta
      901 tttcggtgtc cgcccctgag agatagtgat agcttctcgc gactagaatt ttgatataga
      961 gggcgaagac gacgggcgcg atggagaaga gcgttctcag gatcttggag gcgctctcgt
     1021 cgctctgtac cccctggagc ttcaagaagg tgagtgttcc ggcgagaacg gaggtgtaga
     1081 ccagcgaaaa aaccagcctg cgcttccgaa tgtcctgtat ggcggagacg acctcccgat
     1141 aactccagcg cttgccgctc tcgtagtcgc gccccctgac ggcggcccag acccctccga
     1201 tccatagttc acatcgtttc ccaggttcgc tttcgcgaaa gcttttccct ccctgtagaa
     1261 cgcgctttaa ccacgtttcc ttacgctcag caacagtgag cagcttgccg gcgagcttca
     1321 ttggaagctg aatgtagcgg gtcggaccgg cgtttttccg actcctgagc cgccgaggga
     1381 cggagaacgg cggaatgaat ttttcacaag atctacaagt aaaattttcc caagccagtg
     1441 acaacgagca acgtgctccg atcctatttt tgtcattctc atcacctcat tctttgcgag
     1501 ccgactttcg agtagacatt cagggtgttc gcagtgtttc gcagctactc cgctccgaac
     1561 gcatcaggca aatgaccaga gggagcgaat gcgaaagaag cagtgcgaga accgtatgtt
     1621 tcgcttgcgc aactgctgga gcttgcgcat tcgcgccgcg gtcgcttggc gcgaaagtct
     1681 ccgtcgctca ttctatcatc cgggtggctt tcttcgagga aaacgctgtg cggaagaggg
     1741 aagcgttgct atggtattat cttcgagaat ggtcaaaaag acctcgtgtc ggggagaagt
     1801 ggatgttaag tcgctacgcg cctggacttc caatcatacg agtttagatt tacgaaactg
     1861 cagggtgccg cttcaacagc cgtcgcgctg cttattctcc tcgatacgac aagagagatc
     1921 tttgaacggc cgatctacgc cacctccggt cgcaaatgta cgacgcacac tgtga
//
LOCUS       DAOSWW010000524         1973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47834_length_1973_cov_3.564129, whole genome shotgun sequence.
ACCESSION   DAOSWW010000524 DAOSWW010000000
VERSION     DAOSWW010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47834_length_1973_cov_3.564129"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PK442_10005"
     CDS             complement(<1..852)
                     /locus_tag="PK442_10005"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88029.1"
                     /translation="MATIMFSVLIVLIFLNIPVAVSIGLASIAGITFGNLPLNPAVVA
                     QRMFTSADSFPFMAIPFFMLAGGLMEHGGISRRLVKLASALVGGYRGGLGLITILASA
                     FFGAISGSNPATVAAIGGIMVPSMIKKGYPPAFAASIAAAGGTLGVVIPPSIPMITFG
                     VVAGVSIGDLFLAGFGPGVLLALVLSLVVVMQSIKLDIPKEPAREKGELLAAVKDSIL
                     AIIMPVIILGGIYGGVFTPTEAGAVAVIYSLLVSAFIYEELDLRTIRTVIIKAGISTS
                     VVFFVIAT"
     gene            complement(853..1344)
                     /locus_tag="PK442_10010"
     CDS             complement(853..1344)
                     /locus_tag="PK442_10010"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO88030.1"
                     /translation="MRKVFDNFEEYVLLLLFPLMVIVVFIATLARYFNLFPMFWGEEV
                     ARYIMVFMAYIGAGLGMKKGAHVGVSFFTDKIRGRKLRILFETMRTGIILFFCLMIMY
                     YYKGIIVHQIFMGQTTPALYIPMWIPYAAVPLGMFLVALRAFQAYRETVREIRSAAPS
                     EVK"
     gene            complement(1425..>1973)
                     /gene="dctP"
                     /locus_tag="PK442_10015"
     CDS             complement(1425..>1973)
                     /gene="dctP"
                     /locus_tag="PK442_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO88031.1"
                     /translation="NGFRMLTNSKKPVLVPEDLNGLKIRLMENPIHVGTFKTLGAYPV
                     PMPFGELFTALQQKTVDGQENPLIIISTSKFAEVQKHLSLTGHFYAPAILLINKDFWE
                     KTLSDEQRKIFTEAELKARHWQREFCIDIEKKLVDSLKEQGMEVSQPDKAKFFEAVQP
                     VYKEFEEKVGKEAIQKLIDAQK"
BASE COUNT          446 a          528 c          595 g          404 t
ORIGIN      
        1 cgtggcgatg acgaagaaga caacggacgt gctgatgccc gccttgatga tgaccgtccg
       61 gattgtccgc agatcaagtt cttcgtatat gaacgcgctg actaaaagac tgtagatcac
      121 cgcgaccgcg cccgcctcgg tcggggtgaa gaccccgccg tagatgccgc cgaggatgat
      181 gacaggcatg atgatggcga gaatggagtc cttgactgca gcgaggagtt ctcccttttc
      241 tcttgcaggt tctttcggga tgtcgagctt gatggactgc atcaccacga caaggctgag
      301 gacaagcgcg agaaggacgc cgggaccgaa tcctgcgagg aaaagatcgc cgatggagac
      361 ccctgccaca acgccgaagg taatcatcgg aatggaggga ggaatgacca cgccgagcgt
      421 tcctcctgcg gccgctattg aggctgcgaa tgccggagga taccctttct tgatcatcga
      481 ggggaccatg atgccgccga tggccgccac tgtggccggg ttcgacccgc tgattgcgcc
      541 gaagaacgcg ctcgcgagga ttgtgatcag gccgagccct cccctgtagc cgccgacgag
      601 cgcggatgcg agtttcacaa gtctgcgcga gatgccgccg tgttccatga gtcctcctgc
      661 aagcatgaag aaggggatcg ccatgaaagg gaaggagtcg gcggaggtga acattctttg
      721 tgccacgacg gcaggattca gcggcaggtt gccgaaggtg atccccgcga tggatgccag
      781 tccgatgctc acggccaccg ggatgttcaa aaagatgagc acgatgagga cggagaacat
      841 gatagtggcc atttatttca cctccgaagg ggcggcggaa cggatctccc gcacagtttc
      901 acggtatgcc tggaatgccc gcagcgcgac gaggaacatc ccgagcggga cggctgcata
      961 gggaatccac atggggatgt acagcgccgg agttgtctgt cccatgaaaa tctggtggac
     1021 aatgattccc ttgtagtagt acatgatcat caggcagaag aagaggataa taccggtccg
     1081 catcgtctcg aagaggatgc gaagtttccg gccccggatt ttgtcggtga agaagctcac
     1141 tccgacgtgc gcccccttct tcatccccag tccggcgccg atgtaggcca tgaagaccat
     1201 gatataccgc gctacctcct caccccaaaa catcggaaag aggttgaaat aacgcgccag
     1261 cgtggcgatg aaaaccacga tcaccatcag agggaaaaga agcaacagaa cgtactcctc
     1321 aaaattatcg aagacttttc tcatgaatga cccccgtgtc ggaaaaatgg ctacggggcg
     1381 ctccgcgacg aagcgccccg tacgttattc tatgtaaaaa gaggctactt ttgcgcgtcg
     1441 atgagcttct gaatagcctc tttgccgacc ttttcctcga attccttgta gacgggctgg
     1501 actgcctcga agaactttgc tttgtcgggc tgggatactt ccataccctg ctccttgagc
     1561 gaatccacca gcttcttctc gatgtcgatg cagaactccc gctgccagtg gcgggccttc
     1621 agctcggcct cggtgaagat cttccgctgc tcgtcggaga gcgtcttctc ccagaagtcc
     1681 ttgttgataa gcagaatcgc gggcgcgtag aagtgccccg tgagggagag atgcttctgc
     1741 acctcggcga atttggaggt ggagatgatg atcagagggt tttcctgacc gtcgacagtc
     1801 ttctgctgca gcgcggtgaa gagttcgccg aagggcatcg ggaccgggta ggcgccgagc
     1861 gttttgaagg tgccgacgtg gatcggattc tccatcaaac gaatcttgag cccgttcaaa
     1921 tcttcgggga cgaggacggg ttttttagag ttggtgagca tccggaagcc gtt
//
LOCUS       DAOSWW010000525         1971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47931_length_1971_cov_3.467119, whole genome shotgun sequence.
ACCESSION   DAOSWW010000525 DAOSWW010000000
VERSION     DAOSWW010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47931_length_1971_cov_3.467119"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1862
                     /gene="iorA"
                     /locus_tag="PK442_10020"
                     /pseudo
     CDS             4..1862
                     /gene="iorA"
                     /locus_tag="PK442_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
     gene            1864..>1971
                     /locus_tag="PK442_10025"
     CDS             1864..>1971
                     /locus_tag="PK442_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HOO88032.1"
                     /translation="MQYVIVGIGGQGILFASKVLGAVALNNGQHVMGSEV"
BASE COUNT          388 a          572 c          635 g          376 t
ORIGIN      
        1 agagtgagca caaaagccat actgcttgga aacgaagcga tagcgagagg aattgtggag
       61 gccggatgcc tggttgcgtc ggcctatccg gggacgccgt cgtcggagat tctgcctgcg
      121 gtggcgaagt tcgccgacga gatgaagtcg ccgatgtcgg tggagtggag cgtcaacgag
      181 aaggtggcgt tcgaggtggc cgcggcggct tcgtttactg gagccagggc atgcgcggtg
      241 atgaagcagg tcgggctcaa cgtggcggcc gatccgttca tgagcgtggc gcacttcgac
      301 ctgaagggcg gttttctgct ggtcgtcgcg gacgatccgg gtccgcacag ctcgcagacg
      361 gagcaggaca gccgctactt cgccatgttc gccaagatcc cgtgcttcga ccctgcgacc
      421 gccgccgagg ccaaggagat ggtcttcgac gccttcgacc ctcggagcgt tttggcatcg
      481 tcgcgatgct ccggccgggc gtgcgcgtgg accactgccg tcaggatatc gaacttggag
      541 atattcgccc tgcaaccggt acggtaaagt tcgagaaaga gggaacccgc tgggtctgtc
      601 ttcccgcagg agtgaagatc aaccatccga agctcaatcg caaggttgaa gatatccgga
      661 aggcttttga aaccgagttt tcaaagtaca actacgaggt tccggcgaag aagcaaccgg
      721 tgctcggcat cgtcgcctcc ggcgttccgt tctccgttgt tacggatttg cttaaagaag
      781 ccgggagaga ggatgtctcg gtactgaaaa taggcacgcc gcacccgctg ccggtgaaga
      841 tggtgcagga cttcatcgcc cgtcacgaca gggtgctctt ccttgaagag acctaccccg
      901 tcatggagat gcagcttccc gaccgcacag gtattctcgg ccgctggaac ggcgtggttc
      961 ccagcgcggg cgagctgctt ccggaagtga tcgccgatat tctcggaggg ctcctccagg
     1021 acgattccct tgcgggaaag cagcatcctc ttctgaaaga agcgacggag gatctgaata
     1081 tcgtgccgcg caagccgatg ctctgtcccg gctgcccgca cagggcgagc ttcttctcca
     1141 tccgccaggc gcttccgaac gcgatcaatc cttcggacat cggctgctat acgctcggag
     1201 tcaaccagaa ggccgtggac gcggtcgtcg atatgggcgc ttccgtcaca atggcctcag
     1261 gcttctacct tgcccacaag gccgccggaa tcgaccgccc cgtcgtcacc accatcggag
     1321 actccacctt cttccacatg gggctgcccg gactggcgag cgccgtctac aatcgccacg
     1381 ctttcgtgct ggccatcctt gacaacagca tcaccgccat gacgggcgga caggcgaacc
     1441 ccgccgtcgg aacgaagctc cgcaagggcg acgaaggacg gaaggtcgat atcgaaggcg
     1501 cctgccgggg ctgcggcgtg gagtatgtga agactatcgc cgcctacgac gtagcgcttg
     1561 gcaaggctgc tgtcaaagag gcgtgggagt acgcgaaatc aaaaggggaa ccggccgtgc
     1621 tgatcttccg tcacccgtgc atgctgctcc gtccggagca gccgtcgatc ccggtgaagg
     1681 tcgaccagga gaagtgcatc ggctgcaagt tctgcatcaa cttcttcaac tgcccggggc
     1741 ttgtcttctc cgaggagacg gggaaggcgt acatcgacga acggttctgc gtgtcatgcg
     1801 gcgtctgcgt gtcggtctgc ccgcacggag cgattgtcgc gtcttcggag gaggctgagt
     1861 aagatgcagt acgtcatagt cggtatcggc ggtcaaggaa ttcttttcgc cagcaaggtg
     1921 ctgggcgccg tcgccctgaa caacgggcag cacgtcatgg ggagcgaagt g
//
LOCUS       DAOSWW010000526         1970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_47963_length_1970_cov_3.694517, whole genome shotgun sequence.
ACCESSION   DAOSWW010000526 DAOSWW010000000
VERSION     DAOSWW010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47963_length_1970_cov_3.694517"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..605)
                     /locus_tag="PK442_10030"
     CDS             complement(231..605)
                     /locus_tag="PK442_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOO88033.1"
                     /translation="MKKVIRTDKAPDPIGPYSQGLVCGNRVYVSGQGPRNPETGTSPE
                     GIREQTRQVLKNIKAILEAGGATMDQVVKVTAHLTNLDRDFQGYNEVYAEFFTAPYPV
                     RITVQSQLFGMLVEIDAIAELD"
     gene            750..1406
                     /gene="eda"
                     /locus_tag="PK442_10035"
     CDS             750..1406
                     /gene="eda"
                     /locus_tag="PK442_10035"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOO88034.1"
                     /translation="MNIRKFEVLRRISDLGVVGIIRTPDIEKGVAAAEAVFEGGIDVL
                     EVSTTFPGALDIMKEIIERGRCKGLILGAGTVADATTARLCIDAGAEFIVSHCFSEEV
                     ARMCNRSGIAYMPGVGTVTEVVRAMEHGVDVVKAFPGEVLGPKFIKAVHGPLPGAQIM
                     PIGGVAPGNLDDWFQAGAFAVGLGSALLKPAGTEGDFQAISETAERIVTQIAQIRAHL
                     "
     gene            1437..>1970
                     /locus_tag="PK442_10040"
     CDS             1437..>1970
                     /locus_tag="PK442_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO88035.1"
                     /translation="MSSHWIGVFAALVTGVAWGVVSPLGRMLALRGADMTTVVVVRTL
                     LAACILFLWLLLKNPRSLRISFKDGLVLLAYAVLAIPLTATGFLYSLKYLTVPAALII
                     HYTFPLATLLGGLWITGERPTAPQYIAALLVIAGVWLGVFSSGTADGSFSLLGVFWGL
                     LSVVGLSGQFLMGRVLLS"
BASE COUNT          356 a          611 c          605 g          398 t
ORIGIN      
        1 cggcgccacc gaggtaaaca gcaccgcgga ggacctgcgc aggcttgcgg agcgattcat
       61 cgtcgacgaa gaaagcggcg ggcagctcgc ccttcccggg agaaaatcct gacggaaaaa
      121 gcgtacacgc ccgatgagtt tgaaaaacgc cccctcgctg cgagggggcg tttctgtgcc
      181 tctttacaga actctgataa acgcgggttc agagatccgc tccgccgctc tcagtccagc
      241 tcggcgatgg cgtcgatctc caccagcata ccgaagagct ggctctgcac agtgatcctc
      301 acaggatacg gcgcggtgaa aaactccgcg tacacttcgt tatacccttg aaaatcgcgg
      361 tcgaggttcg tcaggtgcgc cgtcaccttg accacctggt ccatcgtcgc gccgcccgcc
      421 tccaggattg ctttgatgtt cttcaaaacc tgccgtgtct gctcgcgaat cccctcgggg
      481 gacgtcccgg tctccggatt cctggggccc tgtcccgaaa cgtagacgcg gttgccgcag
      541 accagcccct gggagtacgg cccgatggga tccggcgctt tgtcagtacg gataaccttt
      601 ttcactgcaa tcatcctctc gtaagttttt ctccccccct tccgcgaaaa aaacgccgga
      661 catgcaagga aagagacttt ctttgctatc attgtatccg aaaaagctct cgaacgggaa
      721 gccatactct tgtcaggagg atgcaaagaa tgaatatccg aaagtttgaa gtgctgcggc
      781 ggatctccga cctcggcgtg gtagggatca tcaggactcc ggacatcgag aagggcgtgg
      841 cggcggcgga ggcggttttc gaaggaggca tcgacgtgct cgaagtctcc acgaccttcc
      901 cgggcgccct cgacatcatg aaggagatca tcgaacgcgg ccgctgcaaa gggttgatac
      961 tcggcgcggg aacagtcgcc gacgcaacga cggcgcgcct ctgcatcgac gcgggggccg
     1021 agttcatcgt ctctcactgc ttcagcgagg aggttgcgcg gatgtgcaac aggtccggca
     1081 tcgcctacat gcccggcgtg gggacggtca ccgaggtcgt ccgcgcgatg gagcacggcg
     1141 tcgacgtcgt gaaggcgttt ccgggagaag tgctggggcc gaagttcatc aaggcggtcc
     1201 acggtcccct gcccggcgcg cagatcatgc cgatcggcgg cgtggcgccg ggcaatctcg
     1261 acgactggtt ccaggcgggc gccttcgccg tagggctcgg aagcgcgctc ctgaaaccgg
     1321 cgggaacgga aggagacttc caagccatca gcgagacagc cgaacggatc gtcacccaga
     1381 ttgcccagat ccgcgctcat ctataaggcg aagtccgccg caggagggaa gagaggatgt
     1441 catcacactg gataggcgtt ttcgcggcgc tggtcaccgg cgtcgcatgg ggggtcgtca
     1501 gcccgctcgg ccgcatgctc gcccttcggg gcgccgacat gacgaccgtg gtcgtcgtac
     1561 ggacgcttct ggcggcatgc attctctttc tctggctcct gctgaaaaat ccccggtctc
     1621 tccgcatttc tttcaaggac ggattggtgc ttcttgcgta cgccgtcctc gccattccac
     1681 tcacggcgac cggatttttg tattctctga agtaccttac ggttccggcg gcgctcatca
     1741 tccactacac attccccctc gccacgctgc tcggagggct ctggatcacc ggggaacggc
     1801 cgaccgcccc gcagtacatc gccgcgctcc ttgtcatcgc gggcgtctgg ctcggcgtct
     1861 tctcatccgg tacggccgac gggagctttt cgcttctcgg ggtcttctgg gggttgcttt
     1921 cggtcgtcgg gctttcgggc cagtttctga tggggcgggt gttgctctcg
//
LOCUS       DAOSWW010000527         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_48132_length_1966_cov_3.624804, whole genome shotgun sequence.
ACCESSION   DAOSWW010000527 DAOSWW010000000
VERSION     DAOSWW010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48132_length_1966_cov_3.624804"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PK442_10045"
     CDS             complement(<1..602)
                     /locus_tag="PK442_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOO88036.1"
                     /translation="MGVDEFIMVAGLPVGHMQFVEIAREIDKTGIKILVFDEPTAVLT
                     ETEAQNLLAAVKRLAAKGIGIIFISHRLDEIVTVADRVTVLRDGELVATKDIKDTNAV
                     EIAALMIGRKVAISRVQSETRKVSDEIMMSIRDLHVGMPGEMVKGIDLEIKKGEIVGI
                     GGLAGQGKLGIANGVMGTYPSRGEVVFDGKRLNLDDPHAT"
     gene            complement(610..1038)
                     /locus_tag="PK442_10050"
     CDS             complement(610..1038)
                     /locus_tag="PK442_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88037.1"
                     /translation="MSESLLQFKNVSKNYYGNSVLTGINFDVKEGEIHALIGENGAGK
                     STLMNILFGMPVITSTGGYEGDVLIDGKKISFKSPHEAMYAGIGMVHQEFMLIPGFSI
                     TENIKINREITTPNVVSRVLGKRLETLDMVTMNADAQSAG"
     gene            complement(1185..>1966)
                     /locus_tag="PK442_10055"
     CDS             complement(1185..>1966)
                     /locus_tag="PK442_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOO88038.1"
                     /translation="IVYCTPQENPPDVAARAQIIMMPDELAMGNTIPAQAKKLGAKVF
                     VHYSFPRHMSQVLLSSRREIMKKKCAELGLEFVDATAPDPTGDSGLPGAQQFILEDVP
                     KMVAKYGKDTAFFSTNCAMQIPLIKSVVDTGAIYPQPCCPSPYHGFPTALGIESDLSK
                     QDNIKHVIDETRRILKEKGMTGRLSTWPVPVAMMGTVAGTEYAFKVIDGEVPFDKLDV
                     KVLEQCMSDYAKVAVETNPYVDEAGKKYDNFLFVLMDFLTY"
BASE COUNT          402 a          564 c          510 g          490 t
ORIGIN      
        1 atagtagcgt gcgggtcgtc gaggttcaat cgcttgccgt cgaacacgac ttcccctctg
       61 gacgggtaag ttcccatgac gccgttcgct atcccgagtt ttccctgccc ggcgagtccg
      121 cctatcccca cgatctcccc ttttttaatt tcgaggtcga tccccttgac catctcgccg
      181 ggcattccga cgtgcaaatc ccgtatgctc atcatgattt cgtccgatac tttgcgcgtc
      241 tcggactgca ctctgctgat cgccaccttg cggccgatca tcagggcggc tatttccacc
      301 gcgttcgtat ccttgatatc tttggtagcc acaagttctc cgtcgcgcag aaccgtgacc
      361 ctgtccgcga cggtaacgat ctcgtcgagc ctgtggctga tgaaaataat gccgattccc
      421 ttcgcggcaa gccgtttcac cgcggcgagc agattctgcg cctcggtttc ggtaagcacc
      481 gccgtgggct cgtcgaagac gagaatcttg atgcctgtct tatctatctc gcgggctatc
      541 tcgacgaact gcatgtgccc cacgggaagc cccgcgacca tgatgaattc gtcgacgccc
      601 atgtcgagct tatccagcgc tttgcgcgtc ggcgttcata gtgaccatgt cgagcgtttc
      661 aaggcgtttg ccgagaactc tggagacgac gttcggagtg gttatttccc tgttgatttt
      721 aatgttctca gtgattgaaa accccggaat cagcataaac tcctggtgaa ccatgccgat
      781 tcccgcgtac atagcctcat gcggcgactt gaacgaaatc ttctttccgt cgatcagcac
      841 atcgccctcg taaccgccgg tgcttgtgat tacaggcatc ccgaagagga tattcatcag
      901 cgtggacttc ccggcgccgt tctcccctat aagcgcgtgt atctcgccct ctttgacatc
      961 gaagttgatg ccggtcaata cgctgtttcc gtagtagttc ttggaaacat ttttgaactg
     1021 gagaaggctt tcgctcaacg cattaccccc cgtatctcaa aaaatataaa agctgcaata
     1081 aaagagggac gaacggattc gacaccagcg attcgcttcg gtgaaggatc cattcgtccc
     1141 cggacgctac atttgttaca gcttcttttt atgcaaccct tcgattagta ggtcaggaag
     1201 tccataagga cgaacaggaa gttatcgtat ttctttccag cttcgtctac atacggattc
     1261 gtttccaccg cgaccttcgc ataatcggac atgcactgct ccagaacttt gacatcaagc
     1321 ttgtcgaacg ggacttcgcc gtcgatgact ttgaaagcgt actcggtacc ggcgaccgtc
     1381 cccatcattg caaccggcac gggccacgtg gaaagtcttc cagtcatccc cttctccttg
     1441 aggattctgc gcgtctcgtc gataacgtgc ttgatgttgt cctgcttgga caaatcggac
     1501 tcgataccga gagccgtcgg gaatccgtgg tacggagacg ggcagcaggg ctgcggatag
     1561 attgcacctg tgtcgacaac ggacttgatc agaggaatct gcattgcgca gttcgtggag
     1621 aagaacgccg tgtccttgcc gtacttcgcg accatcttcg gcacgtcctc gaggatgaac
     1681 tgctgcgcgc cgggaaggcc ggagtcgcct gtcgggtccg gagccgtcgc gtcgacgaat
     1741 tcaagtccga gttcggcgca cttctttttc ataatctctc tgcgcgagga aagaagaacc
     1801 tgggacatgt gacgcgggaa tgaatagtgg acaaaaacct ttgcgccaag cttcttggcc
     1861 tgggcgggaa tcgtgttacc cattgcgagt tcgtcgggca tcatgataat ctgcgccctc
     1921 gcggccacat cgggaggatt ctcctgcggg gtgcagtaca cgatga
//
LOCUS       DAOSWW010000528         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_48357_length_1960_cov_3.847244, whole genome shotgun sequence.
ACCESSION   DAOSWW010000528 DAOSWW010000000
VERSION     DAOSWW010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48357_length_1960_cov_3.847244"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="PK442_10060"
     CDS             <1..1437
                     /locus_tag="PK442_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO88039.1"
                     /translation="RGSKAAAIVRGEGELAMTAVLEALKAKKSLDAIPGILYIDENGE
                     CVDRGEGPVVENLDELPFPAYEKSMTPVDYSNLYIMTSRGCPYSCVFCHEGALKRPVR
                     RRSVANVVAEMRHLLLKYPELKYFLFCDDTLVANPEWLDEFCREVKALQAVRPFQFYC
                     EADAASLSERPEVIKDMVAAGLNRLQIGIESADREMLKIYRKNLAPEMVETVVKAAYD
                     AGVQQVFGALLVGGPFENREHIEKNKAFAARLMRLGPGMMEIAPSILMPYPMTDIGRH
                     PEKYGLKIYDRQGVASISDYPLMESASMDRREIMAAYQEYIQSFVNEVKAMLDTGELG
                     HEDILRAFRTSMNSKGPKYWSNIIAHHKPMLAPYYTMLAREAASRIGDVQPGELPKWR
                     PQRLLEMWRDVDFSRGYPLARGEALSPFEYEVLKYSTGKSTIAEIAGILYERFGKPFE
                     ETTEDLTGRIVETLKAFDKKYWLLVVPY"
     gene            1450..>1960
                     /locus_tag="PK442_10065"
     CDS             1450..>1960
                     /locus_tag="PK442_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOO88040.1"
                     /translation="MKILLCNVSKKYETAAHDYVGLFYLAGALEAAGFEPLVFHGKAE
                     GLLDEIARHRPEAVGFSCDFDNVRIIEDVSQKIKERWGIPVVVGGPQSIALGRTFLES
                     SGVDYILRGEGEISLPLLLNAVLKGEGELDEVKGLAMIKDGVFHETRPEIIDDLDTLP
                     SPVYHTPLHG"
BASE COUNT          446 a          539 c          593 g          382 t
ORIGIN      
        1 cgcggttcca aagccgccgc catcgtacgg ggcgaaggcg aactggccat gacggcggtt
       61 ttggaggcct tgaaagcaaa gaagagcctg gatgcgattc ctggcatctt gtatatcgac
      121 gaaaacggcg agtgcgtcga caggggcgaa gggccggtag tggaaaatct ggacgagctg
      181 cccttccccg cctatgagaa aagcatgacg cccgtcgatt actccaacct gtacatcatg
      241 accagccggg ggtgccccta cagctgcgtt ttttgtcatg aaggcgcctt gaaacgcccc
      301 gtgcgccggc ggagcgtcgc gaacgtcgtt gcggaaatgc gtcatctcct cctcaagtac
      361 cctgaactca agtacttttt attctgcgac gatacgctgg tcgccaatcc cgaatggctg
      421 gatgaattct gccgggaggt aaaggcgctt caggccgtca ggcccttcca gttctattgc
      481 gaggcggatg cggcgtccct gagcgaaaga cccgaggtca taaaggacat ggtggcggcc
      541 ggactcaatc ggctccaaat cggcatcgaa tccgcggacc gcgagatgct caagatatac
      601 agaaaaaacc tcgctccgga aatggtggaa acggtggtga aagccgccta cgatgccgga
      661 gtgcagcagg ttttcggggc ccttctggta ggcggtcctt ttgagaaccg tgagcatatt
      721 gaaaagaaca aggcctttgc cgccaggctg atgcgcctgg gaccggggat gatggagatt
      781 gcccccagta tcctcatgcc ctatccgatg accgatatcg ggcgccaccc cgaaaaatac
      841 ggactgaaga tctatgacag gcaaggggtg gcatccatct ccgattatcc ccttatggag
      901 agcgcgtcga tggaccggcg ggaaatcatg gcggcctatc aggagtacat tcagagtttt
      961 gtgaacgagg tgaaggcgat gctcgatacc ggcgaactcg gtcacgagga tattctgaga
     1021 gctttccgca cttccatgaa ttcaaaaggt cccaaatact ggagcaacat catagctcat
     1081 cacaaaccca tgctggcccc ctactacacc atgctggcac gggaagcggc ctcccgcatc
     1141 ggcgacgtcc agcctgggga actgcccaag tggcgtccac aacgcctgct cgaaatgtgg
     1201 cgcgacgtgg acttctcccg gggctacccc cttgcgcggg gggaggctct gtcgcctttc
     1261 gagtacgagg tgttgaaata ctccacaggt aagagcacca tcgccgagat agccggcatc
     1321 ctctacgagc gattcggcaa gcccttcgaa gaaacgacgg aggatctgac ggggcgcatc
     1381 gtcgagacct tgaaggcgtt tgataaaaaa tactggctgc ttgtggttcc gtactaagaa
     1441 ggcgccgtga tgaagatact gctatgcaac gtgtcgaaaa aatacgaaac agccgcccat
     1501 gattatgtag ggctctttta cctggcgggc gccttggagg cggcaggatt cgagcctctg
     1561 gtctttcacg gaaaggcgga aggcctcctg gatgagatcg cgagacaccg ccccgaagcg
     1621 gtgggattct cctgcgattt cgacaacgtc cggatcatcg aagacgtctc ccaaaagata
     1681 aaggagcgct gggggattcc cgtcgttgtg ggcggccccc agagcatcgc cttgggaagg
     1741 acgtttcttg aaagcagcgg cgtggactat atcctgcggg gtgaaggcga aatttcgctg
     1801 ccgctgctcc tcaacgcggt cttgaagggc gagggtgaac tcgacgaggt gaaggggctc
     1861 gcgatgatca aggacggcgt ctttcacgaa acacggcccg agatcatcga cgatctggac
     1921 actctgccgt cgccggtcta ccatacgccg ctgcacggcg
//
LOCUS       DAOSWW010000529         1960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_48374_length_1960_cov_2.676115, whole genome shotgun sequence.
ACCESSION   DAOSWW010000529 DAOSWW010000000
VERSION     DAOSWW010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48374_length_1960_cov_2.676115"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK442_10070"
     CDS             <1..189
                     /locus_tag="PK442_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HOO88041.1"
                     /translation="KEVRRMDMGMVNNVMATNQSNTMAQVSTSVQKKAMDMMELQGQM
                     LHEMMASMGIGQNLDIQG"
     gene            complement(252..1898)
                     /locus_tag="PK442_10075"
     CDS             complement(252..1898)
                     /locus_tag="PK442_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOO88042.1"
                     /translation="MSETMNSDMQAPAAGGRSTTIRKGDVLLQLEGGGVWLACRGDSV
                     PFPADVEAALEEGGVTERDEDALRKFLDGKERRVLVASAFVPVDAKVKVAMAKDSMSV
                     SLLVSPPSLGGAMPSPDELEEQLHRHGAVYGIDKSALAKLCAEKLFDEYVEVARGTPA
                     VHGSDAAVEVVVGTGGRRGPVEDEDGNVDLKNLGIVNLVHAGDVLATKTPPTEGVEGH
                     TVRGGVLGAKNGKDKSFPAGPGTVVSEDGLSLLAAVEGHLTEVNGRLQVLPVFEVNGD
                     VDYSTGNIDFPGSVVVKGVVRDGFEVRASENITVNGTVEGAFLVAGGNITVVGGIRGV
                     SKGKVEAKGNIAADFADQATLFADGDILIKNVLLHSDCRRGGKLVVSGGTKSQIAGGK
                     IQAGSEVVCATLGSEMGTKTEIAVGALPEQVERRDFLRKELHQTTENLEKVEKNIAFL
                     KHAEAEKGLDDEKRAMLGKLTRTAFSCKASMKQLGDEAALLEADMERKRERGAVRVRG
                     TCYPGISVSIRGLTYRVREEFKFGALLYDEGEIKVKPFDA"
BASE COUNT          341 a          681 c          554 g          384 t
ORIGIN      
        1 aaggaggtga ggcgcatgga tatgggcatg gtaaacaacg tcatggcgac caaccagtcg
       61 aacacgatgg ctcaggtgag cacttcggtc cagaagaagg ccatggacat gatggaactt
      121 cagggtcaga tgcttcatga gatgatggcc tccatgggga tcggtcagaa cctggacatc
      181 cagggctaga ccgatgcaac gaaggggggc ggaaacgccc cccttcgcat gtgacaacgt
      241 attccgccgc gctacgcgtc gaacggtttg accttgatct ccccttcgtc gtacagaagc
      301 gcgccgaact tgaactcctc ccgcacccgg taggtgagtc cgcggatcga cacggagatg
      361 ccggggtagc aggtacctct gacccgcacc gcgccgcgtt cgcgctttcg ctccatatcg
      421 gcctcgagca gcgcggcctc gtcccccagc tgcttcatcg acgccttgca cgaaaaggcg
      481 gtccgggtga gcttgcccag catcgcgcgc ttttcgtcgt ccagtccctt ttcggcctcc
      541 gcatgcttca ggaaggcgat gttcttctcc accttctcaa gattttccgt cgtctggtgc
      601 agttccttcc tcaggaaatc ccgccgttcc acctgttccg gcagcgcgcc gaccgcgatc
      661 tccgtcttcg tgcccatttc gctgccgagc gtggcgcaga cgacctcgct tccggcctgt
      721 atctttcctc cggcgatctg cgactttgtt ccccccgaga cgacaagctt tcctccgcgc
      781 cggcagtcgc tgtgaagcag cacgttcttg atcaggatgt cgccgtccgc gaagagcgtc
      841 gcctggtcgg cgaagtccgc cgcgatgttg cccttcgcct cgacctttcc cttgctgacg
      901 ccgcggatgc cgccgacgac ggtgatattg cctccggcga ccagaaacgc gccctcgacg
      961 gtgccgttca ccgtgatgtt ctccgaagcg cgaacttcga aaccgtcccg gacgaccccc
     1021 ttcaccacga ccgatccggg aaagtcgatg ttccccgtgc tgtagtcgac gtcgccgttc
     1081 acttcaaaaa ccggaagcac ctggagccgc ccgttgacct ccgtaagatg cccctcgacg
     1141 gcggcgagca gcgaaagccc gtcctcggag acgaccgtcc cggggccggc ggggaacgac
     1201 ttgtccttgc cgttcttggc tccgagaacg ccgccccgga cggtatgccc ctccacgccc
     1261 tccgtcggcg gcgtcttcgt tgcaagcacg tccccggcat gcaccaggtt gacgatgccc
     1321 aaatttttaa gatccacatt gccatcttcg tcctcgaccg gtcccctgcg ccctccggtt
     1381 ccgacgacga cctccacggc cgcatccgac ccgtgaactg cgggtgttcc gcgggcgacc
     1441 tcgacgtact cgtcgaagag cttttcggcg cacagcttcg ccagcgcgct tttgtcgatc
     1501 ccgtacaccg cgccgtgcct gtgaagctgc tcctccagct catcgggcga aggcatggcg
     1561 ccgccgagag atgggggaga aacaagaagc gagaccgaca tggagtcctt cgccatggcg
     1621 accttcacct tcgcgtcgac gggaacgaac gcggaggcga cgagcacccg gcgctccttt
     1681 ccatcgagga acttccggag agcatcttca tctctttcag tcacaccgcc ctcctccagg
     1741 gcggcctcga cgtccgcagg aaagggaacg gaatcgcccc gacaggcgag ccagacgccc
     1801 cctccttcga gctgcaggag gacgtctccc ttccggatcg tcgtcgaccg tccgcctgcg
     1861 gcgggggcct gcatatcgct gttcatcgtc tcgctcatac gcatctccct ttcctgtacg
     1921 ccgcccggga gcggcggaaa acactgtatt ttcatctaat
//
LOCUS       DAOSWW010000530         1953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_48623_length_1953_cov_2.792413, whole genome shotgun sequence.
ACCESSION   DAOSWW010000530 DAOSWW010000000
VERSION     DAOSWW010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48623_length_1953_cov_2.792413"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PK442_10080"
     CDS             <1..562
                     /locus_tag="PK442_10080"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HOO88043.1"
                     /translation="DGIASALEQIGESQSLLHEERNGWTIFRQIFGDRERLQGLYLVR
                     AGAAKAMLYGDDAEEVSYLTANLDEDGDVLDASTRSYALTFAKEELPPVDAFWSITMY
                     SLPDQALVQNPLDRYSLGSKSPSLVYGADGSLTLYLSKESPGESREGNWLPAPDGPFS
                     LTLRMYLPKQDAIPPHYVPPPLRKVR"
     gene            868..>1953
                     /locus_tag="PK442_10085"
     CDS             868..>1953
                     /locus_tag="PK442_10085"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOO88044.1"
                     /translation="MKKRKGLTWYLFLFAVLLLPLSGCSLTNSTENSNEARALAEEAY
                     IFAYPMLENYRTMQVQAISPDRFNRFKHATEIMGPEYKDIVRPNNDTLYSTLWLDLRA
                     EPMVLHIPPITDRYYSFQFVDMYTHNFAYTGVRTTGTRGGVFMVAGPEWNGEKPAGVN
                     EVFRSEGNFVLCLVRTALEGPEDLENVHAIQRRYGVEPLSAFLGVNPPAPSPMDVFPL
                     YLQPAAESVHFINYFNFLLGRLKVHPSEEELIERFGAIGIGPGFYFNADNFSPEIRAA
                     IEAGIASAIKQIEASREILGERKNGWVLTRRIFGNRERMQGLYLVRAGAARMGLYGND
                     LEEAYYPTGNLDADGELFDASKHSYVLR"
BASE COUNT          417 a          543 c          559 g          434 t
ORIGIN      
        1 cgacgggatc gcatctgcgc tagaacagat cggcgagtct cagtcgctgc ttcacgagga
       61 gagaaacggc tggacgatct tcaggcagat cttcggtgac agggaacgct tacaagggct
      121 ctatctcgtc agggccggag ccgccaaggc gatgctctac ggcgacgacg ctgaagaggt
      181 ctcttacctg accgcgaatc ttgatgagga cggcgacgtt ctggacgcct caacgcgttc
      241 atacgctctc accttcgcaa aggaggaact gccccccgtc gacgcattct ggtcgatcac
      301 aatgtactcg ctgcctgatc aggcgctggt acagaatccg ctggacaggt actctcttgg
      361 aagcaagagc ccttcgcttg tctatggcgc cgacggctcg ctgacgttat atctctccaa
      421 ggaatccccc ggcgagtcga gggagggaaa ctggcttccc gcccccgacg gtcccttctc
      481 gctgacgctt cggatgtacc tgccgaaaca ggacgcgatc ccgccgcact atgtgccgcc
      541 tcctttacga aaagtgcgct gaaaaagcga tatccggaga gagcctccct gatcaggagg
      601 ctctctcttt ttcaatgccg gatctcattc cgggcaaaaa tgcgagcgaa gggttccgag
      661 gaatcgcttc atcggttgct tggggatcgc tttgcataaa caaccgacct ccggagtcga
      721 ctcttcagtt ggagggtttt ggtcgcatgt ttttggggcc gaatttatgc ctgaaaatct
      781 gtttttctga tacaatggag ctacgtattt ttactgacct cagcagacct ctatcctggg
      841 ggagagcaac aagaaaggaa gattactatg aaaaaacgga aaggactgac gtggtatctg
      901 tttctttttg cggtgctcct tctgccgctc tcaggatgtt ccctgacgaa cagcacggag
      961 aactccaacg aagcccgcgc gcttgccgag gaagcgtata tcttcgcgta cccgatgctt
     1021 gagaactaca ggacgatgca ggttcaggcg atcagcccgg atcgtttcaa ccgtttcaag
     1081 cacgcaacgg agatcatggg gcccgaatat aaggatattg tccgtccgaa caacgatacg
     1141 ctgtactcta ccctctggct ggacctgagg gcggagccga tggtgctgca tattcctccg
     1201 ataaccgacc gctattattc attccagttt gtggacatgt atacgcataa cttcgcctac
     1261 accggcgtcc ggacgaccgg gacaagaggc ggcgtgttca tggtcgcggg tccggaatgg
     1321 aacggtgaaa aacctgccgg agtgaacgag gtcttccggt cggagggcaa tttcgtcctc
     1381 tgcctggtac ggactgcgct tgaaggaccg gaggacctgg agaatgtgca cgccattcag
     1441 agacggtacg gtgtcgaacc tctgagcgct ttcctgggag ttaacccgcc cgcgccttcc
     1501 ccgatggacg tcttcccgct ctacctgcag cctgccgctg aatccgtcca tttcatcaac
     1561 tactttaact ttcttcttgg ccggctgaag gtccatcctt cggaagagga actgatcgag
     1621 cgtttcggcg ccattggcat cggccccgga ttctacttta acgccgataa tttcagtccg
     1681 gagattcgcg ccgcgatcga agcggggatt gcgtccgcga tcaagcagat cgaggcctcc
     1741 cgggagattc tcggagagag aaagaacgga tgggtgctta ccaggcgtat tttcgggaac
     1801 agggagcgga tgcaggggct ctatctcgtc cgcgccggag ctgcgcgtat ggggttgtac
     1861 gggaacgacc tcgaagaggc atactatcct accggcaatc tcgatgcgga cggcgagctg
     1921 ttcgacgctt cgaagcactc gtatgttctc cgg
//
LOCUS       DAOSWW010000531         1950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_48764_length_1950_cov_2.467546, whole genome shotgun sequence.
ACCESSION   DAOSWW010000531 DAOSWW010000000
VERSION     DAOSWW010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48764_length_1950_cov_2.467546"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>1950
                     /locus_tag="PK442_10090"
     CDS             30..>1950
                     /locus_tag="PK442_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88045.1"
                     /translation="MITVRALDGSGNVTETTAKIVVEAPLPDGKILEPVSGSTLDERH
                     TTLKVRCEANVAAAAYRVNQGPWTPMRVTLGTAEERIPLRFGKNDIEVRLLDAKRAER
                     ALSADVTSTGQRAAEEDTTEKDKKDAPGIPPEAPEVPEAPEAHEEQEPGDETPPQMPE
                     IPGGDEEVDSLLDQIQELMGMPPEGGTPQDHEGPEGAEDGPLPGGDDQEPETAQDPGT
                     IDIPDVGTVDVTGPSDFLLPAEEDEDDDWWLDAGGDLEEEEPLEPEEIEIEEEGGEDV
                     GDEPDDEEITGEDFFTETDLFDGFDGLDEEAEPLDLEAWVEPKGNPDAPEQDPMYECP
                     LEPFDAADTDPSSPFTPPSGLQNDPGGCVAVETVQKDWYCTNRPNINVKFQLPDWLKK
                     LDLPKPGTKEYDEMVKKLLQHLRDRGIDTAAFERFQDALIRRAGRLEHPDELPTFLQS
                     LGLTSMPKPSPDDVEANKRWREAMENGARAWYLRLLASGDPQMIYQGLKARGEALGKF
                     DEALGLAADAMMTEIKANQQLTEQALWSLPYVGPAMDVLSIITGETLSGEAMTPERAA
                     MMLGLRGGHVKLDGEDISHLSPQDRVARGMAFVPQTNNVFPSMSVEENLEMGAFLRRD
                     DISGTMRQVFELFPIL"
BASE COUNT          419 a          584 c          684 g          263 t
ORIGIN      
        1 caaaaggagt aatccagccg ggcgagcgcg tgatcacggt ccgtgcgctc gacggcagcg
       61 gcaacgtcac ggagacgacg gcaaagatcg tcgtggaggc cccgctcccc gacggaaaga
      121 ttctggagcc ggtatccggc agcactctcg acgagcggca cacgacgctg aaggtgcgct
      181 gcgaagcgaa cgtggccgcg gcggcgtaca gagtgaatca ggggccgtgg acgccgatgc
      241 gcgtgacgct ggggacggcg gaggagcgta ttccgctgcg cttcgggaaa aacgacatcg
      301 aagtcaggct gctcgacgcg aagagagcgg aacgggcgct ctccgcggac gtgacctcca
      361 ccgggcagcg tgcggcggag gaagacacca cagaaaagga caaaaaggat gcgccgggga
      421 tacctccgga agcgcctgaa gtgcctgaag cgcctgaagc gcatgaggag caagagccag
      481 gagatgaaac gcctccccaa atgccggaga ttcccggcgg ggacgaagag gtcgactccc
      541 ttctcgacca gatacaagag ctcatgggaa tgccccccga gggcgggacg cctcaggatc
      601 atgaggggcc ggagggtgcg gaagacggtc ctctgcccgg aggggatgat caggagccgg
      661 agacggcgca ggatcccggg acgatcgaca ttcccgatgt gggaaccgtc gatgtgacgg
      721 ggccctccga cttcctgctg cctgccgagg aggacgagga cgacgactgg tggctcgacg
      781 cgggcggcga tcttgaagag gaagagccgc tggaacccga ggagatcgag atcgaggaag
      841 agggcggcga ggacgtcggc gacgaacccg acgacgagga gattaccgga gaggacttct
      901 tcaccgaaac cgacctcttc gacggattcg acgggctgga cgaagaagcg gagcctctgg
      961 acctcgaagc gtgggtggag ccgaagggca atccggacgc tcccgagcag gacccgatgt
     1021 acgagtgccc cctggaaccg ttcgacgccg ccgacaccga cccgagctcg cccttcacgc
     1081 caccgtcggg gctgcaaaac gatccgggcg gctgcgtggc cgtggaaacc gtccagaaag
     1141 actggtactg caccaacagg ccgaacatca atgtgaagtt ccagctcccc gactggctga
     1201 agaagctcga cctgcccaag ccggggacga aagagtacga cgaaatggtg aagaagcttc
     1261 tgcagcatct gcgcgacagg ggcatcgaca ccgcggcgtt tgaacgtttc caggacgccc
     1321 tgatccggag ggcggggagg ctggagcacc ccgacgagct gccgacgttc ctgcagtcgc
     1381 tcgggctgac ctccatgccc aagccgtcgc cggacgacgt cgaggcgaac aagcgctggc
     1441 gcgaagccat ggagaacggc gcccgcgcat ggtatctccg cctgctggcc tccggcgatc
     1501 cgcagatgat ctaccagggc ctgaaggcgc gcggtgaagc gttgggcaag ttcgacgaag
     1561 cgctcggcct ggccgccgac gccatgatga cggagatcaa ggcgaatcag cagctgacgg
     1621 agcaggcgct ctggtcgctg ccctacgtcg gcccggcgat ggacgtgctc tcgatcatca
     1681 ccggggagac gctctccggc gaggcaatga ctccggagcg ggccgcgatg atgctcgggc
     1741 tgcgcggcgg ccatgtgaag ctcgacggcg aggatatcag ccatctgtcg ccgcaagaca
     1801 gggtggccag gggcatggcc ttcgtgcccc agaccaacaa tgtctttccg tcgatgtcgg
     1861 tcgaggaaaa cctggaaatg ggcgccttcc tgcgccgcga cgatatcagc ggcaccatga
     1921 ggcaggtttt cgagctgttc ccaatcctga
//
LOCUS       DAOSWW010000532         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_48881_length_1947_cov_3.446617, whole genome shotgun sequence.
ACCESSION   DAOSWW010000532 DAOSWW010000000
VERSION     DAOSWW010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48881_length_1947_cov_3.446617"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1947)
                     /locus_tag="PK442_10095"
     CDS             complement(<1..>1947)
                     /locus_tag="PK442_10095"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOO88046.1"
                     /translation="RRDPALRMPYDDSLGALLHEPTFQNLLPQAVFQKARVIQRMGNR
                     AVHDTRPVRPSDALLAVKELFHVCYWLTRTYAPGAVLPDAAWRDDRVPEARGGDAVPR
                     AELEALEEKLAGQSRLALEERRERDALDAEVRELRARLAEIRAASEAVPDMHDYSEAE
                     TRRWLIDAELRRAGWPLDSPRDREYRVTGMPNAQGEGFADYVLWGDDGKPLAVVEAKR
                     TTADPAAGRQQAKLYADCLEAMHGQRPVIFFTNGYETWLWDDAFYPPRTVAGFFTKDE
                     LARLILRRSQRGALDVAQVRDAIVERYYQKRAIGSIFAQFAASRRKALLVMATGTGKT
                     RTAIALVDALQRAGWVKRALFLADRVSLVHQAVNAFKRHLPESSPVNLVTDKEAEGRV
                     FVCTYPTMMGLIDTTDGEAARFGPGHFDLVIIDEAHRSVYQKYGAIFRYFDSLLVGLT
                     ATPRDDVDKNTYDLFELEPGVPTDAYELETAVADGFLVPYKVRKPELKFPREGIVYDS
                     LTDEEKAQWESLDWGDEAEERGLPERVNASAVNSWLFNCDTVDKALGYLMEHGHAVKG
                     GDRLAKTILFARNHEHAQFVERRFDAHYPHYAGSFARVIDNYARYPQSLIDDFAQPDK
                     PPHIAISVDMLDTGIDIPEVANLV"
BASE COUNT          296 a          698 c          610 g          343 t
ORIGIN      
        1 agacgagatt cgccacctcg gggatgtcga tgccggtgtc cagcatgtcc acggagatgg
       61 cgatgtgcgg cggcttgtcg ggttgggcga agtcgtcgat caggctctgc ggataccttg
      121 cgtagttgtc gatcacgcgc gcgaagctcc ccgcgtagtg cgggtagtgc gcgtcgaagc
      181 gccgttcgac gaactgcgca tgctcatggt tccgggcgaa gagaatcgtc ttggcgagcc
      241 ggtcgccgcc cttcaccgca tgcccgtgct ccatcaggta cccgagcgcc ttgtccaccg
      301 tatcgcagtt gaagagccag ctgttcaccg ccgaggcgtt caccctctcg ggaagccctc
      361 gctcctccgc ttcgtcaccc cagtccaggc tctcccactg ggccttctct tcatccgtca
      421 gggaatcgta gacgatcccc tcgcggggaa acttcagctc cggcttccgc accttgtacg
      481 ggacgaggaa accgtcggca accgccgtct ccagctcgta ggcgtcggtc ggcacgcccg
      541 gctccagctc gaagaggtcg taggtgttct tgtccacgtc gtcgcgcggc gtcgccgtca
      601 gtcccacgag cagcgagtcg aagtagcgga agatcgcgcc gtacttctgg tagaccgaac
      661 ggtgggcctc gtcgatgatc acgaggtcga agtgccccgg cccgaagcgc gccgcctccc
      721 cgtccgtcgt gtcgatcagc cccatcatcg tcggataggt gcagacgaag acgcgccctt
      781 cggcctcctt gtcggtcacc aggttcacgg ggctcgactc cggcaagtgc ctcttgaagg
      841 cgttcaccgc ctgatgcacc agagaaaccc ggtccgccag aaagagcgcg cgcttcaccc
      901 atccggcccg ctgcagcgcg tccaccagag cgatggccgt gcgcgtcttg cccgtccccg
      961 tcgccatcac gagcagcgcc ttgcgccgcg atgccgcgaa ctgcgcgaag atgctgccga
     1021 tggcgcgctt ctgatagtag cgttcgacga tggcgtcccg cacctgcgcc acgtcgagcg
     1081 ccccccgctg cgaacggcgc aggatcagcc gcgccagctc gtccttcgtg aagaaccccg
     1141 ccaccgtgcg cggcggatag aatgcgtcgt cccacagcca cgtctcgtac ccgttggtga
     1201 agaagatcac cggccgctgc ccgtgcatcg cctcaaggca gtcggcgtac agcttcgcct
     1261 gctggcgtcc cgcggcgggg tccgcggtcg tccgcttcgc ctccaccacg gccagaggtt
     1321 tcccgtcgtc gccccagaga acgtagtcag cgaacccctc gccctgtgcg ttcggcatcc
     1381 ccgtaacccg gtattcccgg tcgcggggac tgtcgagcgg ccacccggct cggcgcagct
     1441 ccgcgtcgat cagccagcgc cgcgtttcgg cctccgagta gtcgtgcata tcggggacgg
     1501 cttcgctcgc ggcgcggatt tccgccagcc gtgcacgcag ctcgcgcacc tccgcgtcca
     1561 gcgcgtcgcg ctccctccgc tcttcgaggg caagcctgct ctgcccggca agcttttcct
     1621 caagcgcttc aagttcagcg cgcggaaccg cgtccccgcc ccttgcctct ggcactcgat
     1681 catcccgcca cgcggcgtcg ggcagtacgg cgccgggagc gtaggttcgc gtcagccagt
     1741 agcagacgtg gaacagctcc ttcaccgcga gcagtgcgtc cgacgggcga accggacggg
     1801 tgtcgtgcac ggcgcgattt cccatgcgct ggatcacccg cgccttctga aagaccgcct
     1861 gcggaagcag attctgaaag gtgggctcgt ggagcagcgc gccgaggctg tcgtcgtacg
     1921 gcatccgaag cgccgggtcg cgcctga
//
LOCUS       DAOSWW010000533         1945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_48967_length_1945_cov_3.073016, whole genome shotgun sequence.
ACCESSION   DAOSWW010000533 DAOSWW010000000
VERSION     DAOSWW010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48967_length_1945_cov_3.073016"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PK442_10100"
     CDS             <1..777
                     /locus_tag="PK442_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88047.1"
                     /translation="DCTANPRAAQRLIGVVPDESNLYPELTGFENLAFCASLYGMRKA
                     ERRKRATELLDMFGLSKAGFRKFGGYSKGMKRKLTIAAGIIHRPRVLFLDEPTTGIDV
                     ASARQVRRLIADLHREGTTIFLTTHFIEEAERLCERIAFLVQGRIERIDTVENLLQPL
                     REKHVVHIACAVVLDDVRRALSENFPEASFACMEAGRIRVESEEPIAVGKLVRFLEEN
                     EVEVYEARKATPSLEEVFVRITGIEAEEMRAERSGPGKAR"
     gene            complement(858..1580)
                     /locus_tag="PK442_10105"
     CDS             complement(858..1580)
                     /locus_tag="PK442_10105"
                     /inference="COORDINATES: protein motif:HMM:NF023540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HOO88048.1"
                     /translation="MSGISAVPPVNSIKTVVPVANRTRREGLQRAEREVMRQELAVRN
                     QGDVTSTVYHYEIGPDGRRYITGATVTYKEKENELSSKSAKDKSSASSANTGPASSAE
                     NGRVELTDESRAKLKAIERDVIAHEAAHKAVGGQYAGPVSYTYATAPDGTRYIVGGEV
                     SISAPEGKTPEETIQIMEQVKRAALAAGSPSPQDIQVAAAASAAQMRARAEMAKQEAE
                     KAYSGEKGAWDFSRWGEFAMSA"
     gene            complement(1639..>1945)
                     /locus_tag="PK442_10110"
     CDS             complement(1639..>1945)
                     /locus_tag="PK442_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88049.1"
                     /translation="GVPSAAEAFEALRNEVYDAIFMDLHMPEMNGCEASMFIRRSGAG
                     EANRSLPIFAITGSDLPEERESCVSAGMTDFLAKPFSGEHAEALLRKHCGKTAHDGQ"
BASE COUNT          371 a          556 c          592 g          426 t
ORIGIN      
        1 gactgcaccg caaatccgcg ggcggcacag cggctgatcg gcgttgtgcc ggacgagagt
       61 aatctttatc ccgagctgac cggattcgag aacctcgcgt tctgcgcctc gctctacggt
      121 atgcgcaagg ccgaacggag gaaacgggct acggagcttc tcgacatgtt cggactgagc
      181 aaagccggat tcaggaagtt cggcggctac tccaagggga tgaagcggaa gctgaccata
      241 gccgccggga tcatccaccg tccgcgcgtc ctcttcctgg acgagccaac cacaggaatc
      301 gacgtcgcga gcgcgaggca ggtacggcgc ctcatcgccg atcttcatcg ggaggggacg
      361 accatctttc tgacgacgca cttcattgag gaggcggagc gcctgtgcga aagaatcgcc
      421 tttcttgtgc aggggaggat cgagcggatc gacacggtcg agaatctgtt gcagccgctc
      481 cgggagaagc acgtcgtgca catagcatgc gcggtcgtgc tggacgatgt gcgtcgagcc
      541 ctctccgaaa acttcccgga ggcgtcgttt gcctgcatgg aggctgggag aatccgtgtc
      601 gaaagcgagg agccgatagc ggtcggcaag cttgtccgct tccttgaaga gaatgaggtc
      661 gaggtgtacg aggcgaggaa ggcaacgccc tccctggagg aggtcttcgt tcgcatcacc
      721 ggcatcgagg cggaggagat gcgcgcggag agaagcggcc ccggaaaggc tcgatgaagt
      781 cctgggaaaa agagcataag aaagacgtcc gtgaacggga cgtctttctt atcgtctgaa
      841 gatgaaaagg aaccaggtta cgccgacatc gcgaactcgc cccacctgga gaagtcccat
      901 gcgccttttt cgccggagta ggctttttca gcctcttgct ttgccatctc ggcgcgtgcg
      961 cgcatctggg ccgcagaagc tgcggccgcg acctgtatat cctgcggaga tggagaacct
     1021 gccgccagcg ccgcccgctt tacctgctcc attatctgga ttgtctcttc cggggtcttc
     1081 ccttcgggag cggagatgga gacctcgccg ccgacgatgt agcgcgtgcc gtccggagct
     1141 gtggcgtacg tataggaaac cggtccggca tactgtccgc cgactgcctt gtgggcggct
     1201 tcgtgcgcga tgacgtcgcg ttcgatcgcc ttcaacttgg cccggctctc gtcagtgagc
     1261 tcgacgcggc cgttttctgc ggaggaggcg ggtcctgtat ttgcagagct cgctgaagat
     1321 ttgtccttcg cagactttga ggagagctcg ttttcttttt ctttgtaggt taccgtagct
     1381 ccggtaatgt agcgccttcc gtcgggacct atttcgtagt ggtagacggt ggaggtgacg
     1441 tcgccctggt tcctcacggc cagctcctgg cgcatgactt cccgttcggc gcgctgcaaa
     1501 ccttcacgcc gggttctgtt ggctacggga acgactgtct tgatgctgtt tacaggaggt
     1561 acagccgaaa taccggacat atccattgcc tcctcccttt aaaaactatc tattcaacca
     1621 attatagcac accccggatt attgtccatc gtgtgcagtc tttccgcaat gttttctcag
     1681 cagcgcttcc gcatgttctc cggagaacgg ttttgccagg aaatctgtca tacccgccga
     1741 tacgcagctt tctcgttcct cgggcagatc gcttccagta atcgcgaaaa tcggcagaga
     1801 gcggttggct tctccggcgc ctgaccggcg gatgaacatc gacgcttcgc agccgttcat
     1861 ttccggcatg tgcagatcca taaagattgc gtcgtagact tcatttcgca gcgcttcgaa
     1921 ggcctcggcg gccgagggta ctcct
//
LOCUS       DAOSWW010000534         1943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49034_length_1943_cov_4.319915, whole genome shotgun sequence.
ACCESSION   DAOSWW010000534 DAOSWW010000000
VERSION     DAOSWW010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49034_length_1943_cov_4.319915"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PK442_10115"
     CDS             <1..591
                     /locus_tag="PK442_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOO88050.1"
                     /translation="LLLIVLGSGWYVWYSWEQSGVSLFSLNNDRESVSEEERSENEGN
                     GRTSEQSLTLEPPAETSDPQSGEISGDSSMAIGAPDTHPTFVLPPTAAELVGVAETPV
                     ATPTPVVAEKKEKELVITANADCWVRVRQGTKTVFERTMRSGDTADFKVTERLSVTFG
                     RGSAIKVTWNGKDLGAPGRGVERIFYDPDGTTGRFD"
     gene            591..1883
                     /gene="rimO"
                     /locus_tag="PK442_10120"
     CDS             591..1883
                     /gene="rimO"
                     /locus_tag="PK442_10120"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOO88051.1"
                     /translation="MKVYLISLGCAKNSVDSEKLLGVLQAFGVPVVHDIAEADIAIVN
                     TCGFIQSAVEENVNVILDLESMKEKGLLKKIGVVGCLVNRYGDELKKELPTVDLWAKA
                     EEWGNVAAFFGGSMTFEGKRGILQETRPWSRYLKVGEGCDTFCSYCTIPSIRGKARSI
                     PLPELLQEAQSLVQNGAKELCLVGQDLTIYGRDLYGDDGLRTLLRALDAELPEEVWIR
                     MLYLHPSRIDESFIDMVAENPRILSYLDIPIQHIDDEILHRMNRPDKEGHIRRIFAYA
                     RSVDPLFALRTTIITGFPGETERQFNKVLNFLEEAEIDRVGAFMYSPEEGTPAAQMEG
                     QVDASVKNSRYDRLMTLQAEISLERQRLFLGRTLSILVDEISQDDGFAWGRSYRDAPE
                     VDGLVGVAEGAGLTEGAFVEVLITDAEEHDLFAEISGN"
BASE COUNT          504 a          406 c          538 g          495 t
ORIGIN      
        1 cttttgctga tcgttttggg gtcgggatgg tatgtttggt attcgtggga acagagcggc
       61 gtttccctct tttcactgaa caatgatcgg gaatctgtgt ccgaagaaga acgatctgaa
      121 aacgaaggga acggacgaac ttcagaacag agcctaaccc ttgaaccccc tgctgaaacg
      181 tctgatcctc aatcaggtga aatcagtgga gattcatcta tggcaatcgg ggctcctgat
      241 acgcatccga cttttgttct ccctcccaca gctgcggagc ttgtaggcgt tgcggaaaca
      301 cctgttgcta cgccgacgcc tgtagttgca gaaaagaagg agaaagagct ggttatcacg
      361 gccaacgccg attgctgggt acgtgttcgt caaggaacca aaacggtgtt tgagcgtacg
      421 atgcgatccg gagatactgc cgattttaag gtaactgaaa gactgagtgt tacttttgga
      481 cggggaagcg ccattaaagt gacatggaac ggcaaggatt taggggctcc cggacgagga
      541 gtcgaaagaa tattttatga tccggacgga acaacgggaa ggttcgatta gtgaaagttt
      601 atctaatcag tttaggatgc gcaaagaact ccgtggacag cgaaaagctc ctcggagttc
      661 ttcaagcttt cggagtgcct gttgtgcacg atattgccga ggccgatatc gccattgtga
      721 acacatgcgg ctttattcag tctgccgtcg aggagaatgt taatgttatc ctcgatcttg
      781 agtcaatgaa agaaaaaggg cttcttaaaa aaatcggcgt agtcggttgc cttgtcaaca
      841 gatatggcga cgagttgaaa aaagagcttc caacggttga tttgtgggca aaagctgaag
      901 agtgggggaa cgttgccgcc tttttcggag gaagtatgac cttcgagggg aaaaggggta
      961 ttctacaaga aacgcgccct tggtcacgct atctaaaagt gggtgaaggg tgcgacactt
     1021 tttgttctta ctgcactatc ccttcgatta gaggaaaggc tcgcagcatt cctcttcctg
     1081 aacttctcca agaagcgcaa agccttgttc agaacggagc gaaggaacta tgtcttgtag
     1141 gacaggatct tacaatttac ggaagagatc tttacggaga tgatggactt cgtacgcttt
     1201 tgcgagctct tgacgctgag cttccggaag aagtttggat tcgcatgctg tatcttcatc
     1261 cttcaagaat agacgaatca ttcattgata tggttgccga gaatccgaga attttgtcct
     1321 atctggacat accgatacaa cacattgatg atgagatttt gcacagaatg aaccgtccgg
     1381 acaaagaggg acacattcgt cgtatatttg cttacgcgag gagcgtcgat ccgctctttg
     1441 cgcttcgtac aacgattatt accggtttcc cgggggaaac cgagcgacag ttcaacaaag
     1501 tgctcaattt tcttgaagag gccgaaatag atcgcgttgg tgcatttatg tattctcccg
     1561 aagagggaac gccggcggct caaatggagg gacaggtcga tgcgtcggta aaaaacagcc
     1621 gctacgaccg tttaatgacg ctacaggctg agatatccct ggagagacaa cgactttttt
     1681 taggacgtac gctttcaatt ctggtcgatg aaatcagcca ggacgacgga tttgcatggg
     1741 ggcgttccta cagagatgct cccgaggttg atggtctcgt aggcgttgcc gaaggcgccg
     1801 ggttgacaga gggagctttt gtggaggtgt tgataacaga tgctgaagaa catgatctct
     1861 tcgcggaaat atcagggaac taaagatatc gcatggtgta tcagcactct ttgcggattg
     1921 ggttttttct cttcgatgcc ggg
//
LOCUS       DAOSWW010000535         1939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49218_length_1939_cov_3.143312, whole genome shotgun sequence.
ACCESSION   DAOSWW010000535 DAOSWW010000000
VERSION     DAOSWW010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49218_length_1939_cov_3.143312"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..650)
                     /locus_tag="PK442_10125"
     CDS             complement(192..650)
                     /locus_tag="PK442_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88052.1"
                     /translation="MMRYRIIAWTLLLFFAACCVPAESVMPPEVYANMAEKSRIKATA
                     VVESVKALETGKQSTWKSVVFKLRFPFTENVPEQFTGTCYSVDFPWQEPPVGGTLYYY
                     PEAGDLVYVTVGSNGGPITSYTYLNDVMESLFIDNEEKIRYGMGNAWLDL"
     gene            830..>1939
                     /locus_tag="PK442_10130"
     CDS             830..>1939
                     /locus_tag="PK442_10130"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOO88053.1"
                     /translation="MTNKWRSSEVFAGAPNAGARALWKSMGYCDGDFAERPVIGIANS
                     WNTLVPGHFNLNQVAEQVKKGIHRAGGVAAEFGVIASCDGVCEGHRGMHFILPQREVI
                     ADSVELVVEAHHMDAIVLLASCDKIVPGMLMAAARLDIPAIMITGGPMLGGVEFDGRK
                     TDETSLHEALGMLTAGRISDEEFHSLENLCAPTCGSCSFFGTANTMCCLAEVLGMTLP
                     GSALVPAVHAERLRLACATGERICEMAREGLTARKILNASSIVNGVRATLAMSGSTNA
                     VMHLAAIAAEAEAGVDVMELFSRLEQETPQIVRINPASKWNTEDFWMAGGIPRMLKNM
                     LPIIEKDAMTCTGHTVEGNVKAFRFAFPKNDEVMKT"
BASE COUNT          393 a          600 c          580 g          366 t
ORIGIN      
        1 ggagcgcact tgaccgtata tgtaaaaggg tgcggaccgc cgacgacgcc tgtggtcgag
       61 tagtccgccg aagaaggccc tgcggccgca aaaaacacga tcaccactga aagcaggaaa
      121 cacgcgacac atcgtcctct actcgctctt ctcatgccca actcctttcc ggaaacgccc
      181 ctggctgcac attaaagatc cagccaggcg ttccccatgc cgtagcgtat tttttcttca
      241 ttgtcgataa agaggctctc catcacatcg ttcaggtacg tgtagctcgt gatcggaccg
      301 ccgttcgacc ccaccgtgac atagacgaga tcccccgctt cagggtagta atagagcgtc
      361 ccgcccacag gcggctcctg ccaggggaag tcgacggagt aacaggttcc ggtgaactgc
      421 tccggcacgt tctccgtgaa cgggaatctc agtttgaaga caacgctctt ccatgtgctc
      481 tgcttccccg tctccaaagc cttcacggac tcgacaaccg ccgtcgcctt gatcctcgac
      541 ttttcggcca tgttcgcgta gacctccgga ggcatcacgc tctccgcggg gacgcagcac
      601 gccgcaaaga acagaagcag cgtccacgca ataatccggt aacgcatcat gaatcctccc
      661 tcctctcatt cggattttta gcgcatcgcg cagtcttttt cagtatagcc tctcctgagg
      721 atatgcgcat cgtgattttc gcttttttgg attttcgcag cctgttgacg aaacgcccgc
      781 tctcccctac tatgcagaag aattgcaatt ttccggagga ggaccgttca tgacaaataa
      841 atggagaagc tctgaggtct ttgcgggtgc gccgaatgcc ggggcgcggg cgctgtggaa
      901 gtcgatgggg tactgcgacg gtgatttcgc cgaacggccg gtcatcggca tcgcgaactc
      961 gtggaacacg ctggtcccgg ggcacttcaa cctgaaccag gtcgccgaac aggtgaagaa
     1021 agggatccac cgcgcagggg gggtggcggc ggagttcggc gtcatcgcat cgtgcgacgg
     1081 cgtctgcgag gggcacaggg ggatgcactt catccttccg cagcgcgagg tcatcgccga
     1141 ctcggtcgag ctcgtcgtcg aggcgcacca catggacgcg atcgtcctgc tggcctcgtg
     1201 cgacaagatc gtccccggca tgctgatggc ggcggcccgg ctcgacatcc ccgcaatcat
     1261 gatcaccggc ggaccgatgc tcggcggcgt ggagttcgac ggacgcaaga ccgacgaaac
     1321 gtcgctccac gaggcgctgg gaatgctgac ggcggggcgc atctccgatg aggagtttca
     1381 ttcgctcgaa aacctctgcg cgcccacctg cggctcctgc tccttcttcg gcaccgcgaa
     1441 cacgatgtgc tgcctcgccg aagtcctcgg gatgacgctt ccgggaagcg cgctggtacc
     1501 cgcggtccac gcggaacggc tgcgactggc ctgcgccacg ggagagcgta tctgcgagat
     1561 ggcccgcgag gggttgacgg cgcggaagat actgaacgcc tcctccattg tgaacggcgt
     1621 gcgggcgacg ctcgccatga gcggttcgac caacgcggtg atgcacctcg ccgccatcgc
     1681 cgcggaggcg gaagccggcg tcgatgtgat ggagctattc tcccgccttg aacaggagac
     1741 gccgcagatc gtgcggatca accccgcgtc gaagtggaac accgaggact tctggatggc
     1801 ggggggcatc ccccggatgt tgaagaacat gctcccgatc atcgaaaagg acgccatgac
     1861 ctgtacgggg cacaccgtgg aaggaaacgt aaaagccttc cgcttcgcct tcccgaagaa
     1921 cgacgaagtg atgaaaacg
//
LOCUS       DAOSWW010000536         1937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49300_length_1937_cov_3.907545, whole genome shotgun sequence.
ACCESSION   DAOSWW010000536 DAOSWW010000000
VERSION     DAOSWW010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49300_length_1937_cov_3.907545"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="PK442_10135"
     CDS             complement(<1..1274)
                     /locus_tag="PK442_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOO88054.1"
                     /translation="MKQRMRSTVRSVFLVCFLTVFLSAPEASGADGRVVIFALNDMHS
                     QLLPVTTKVKTTTVLFGGLAHAGGVIAEQRAAEKDPVLALQAGDMVAGLMWRLFSGEP
                     EFSALEHAGVQAGTLGNHEFDYGVEHLKKLLSFTSMPMVVSNLEFDDDFLKQRVLKRL
                     MLQAGAMKVGVFGLASPNLFALAGAGDSVKVDAALRQISEKMVAGLREEGADAILLLS
                     RLSDEENEALAAGVEGIHAILGGSSHNELHETRIVEGPNGWKTALGRAGAYCAFVGRL
                     RLAVKDGKLDEQETEWSLLRVTPRARTDGDVLALALEYERKLNEAMLFTIGTFSDAAD
                     ARAQTVRARESALGNLIADSLRWRMKTDVAVINGGGVRGDKIFPAGSVSRKVLAEILP
                     FDNEIHIVLLTGEQLRKVFEIAASSLVGKNDR"
     gene            complement(1279..>1937)
                     /locus_tag="PK442_10140"
     CDS             complement(1279..>1937)
                     /locus_tag="PK442_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO88055.1"
                     /translation="LSRWKHPQKGILPPAAFIPQAEQTGFVANLDTRMLEHALDAVNT
                     ARRLNPQLRLFFSSNASSVFLRSAAAVESVKFFLESTGADPTRFTLEVTESSLIENLD
                     DVHVKLDLLKESGIRIALDDFGTGYSSLQYINNLPLDVIKLDRAFVSQLFVSAKTERM
                     MRSIITMGRDLGLDIIAEGVETAEQLAWLKSAGCPKAQGYYFSPPVPWDEAQRMIQEV
                     "
BASE COUNT          366 a          642 c          561 g          368 t
ORIGIN      
        1 tacctgtcgt tctttcccac aagagaggag gctgcgattt caaagacctt ccggagctgt
       61 tcgcccgtca ggaggacaat gtgaatctcg ttgtcgaagg ggagaatctc cgccagcact
      121 ttccgcgaga cgcttcccgc agggaatatc ttgtcccccc tgacgccccc gccgttgatg
      181 acggcgacgt cggtcttcat cctccaacgg agcgaatcgg caatcaggtt gccgagggcg
      241 gattccctgg cgcgcaccgt ctgcgcgcgg gcgtccgcgg cgtcggagaa ggtcccgatg
      301 gtgaagagca tcgcctcgtt cagcttgcgc tcgtattcaa gcgcaagcgc cagcacgtcg
      361 ccgtcggtcc gggcgcgcgg cgtcacgcgg agcaggctcc attcggtctc ttgttcgtcg
      421 agcttcccgt ccttgacggc gagccggagc ctgcccacga aggcgcagta agcgcccgcg
      481 cgtcctagcg ccgtcttcca gccgttcggc ccctcgacaa tgcgcgtctc gtgcagctcg
      541 ttgtgggagg agccccccag tattgcgtga atcccctcca ccccggcggc cagcgcctcg
      601 ttctcctcgt cggagaggcg gctcagcaac agaatggcgt ccgccccctc ttcccggagc
      661 ccggcgacca tcttttcgga gatctgccgc agcgctgcgt ccaccttcac ggaatcgccc
      721 gcgcccgcca gagcgaagag attcggcgaa gccagtccga aaacgcccac cttcatggcg
      781 cccgcctgaa gcatgagtcg tttcaaaacc cgctgtttca aaaagtcgtc gtcgaactcc
      841 aggttggaga cgaccatcgg catggaggta aacgagagca gttttttgag atgctccacg
      901 ccgtaatcga actcgtgatt gcccaaagtc cccgcctgca cgcccgcgtg ctccagcgcc
      961 gagaactccg gctccccgga gaagagccgc cacataaggc cggcaaccat atccccggcc
     1021 tgaagcgcaa gcaccgggtc tttctcggcg gcgcgctgct ccgcgatgac gccgcccgca
     1081 tgggcaagcc ctccgaagag caccgtcgtc gtcttcactt ttgtggtcac gggaagaagc
     1141 tggctatgca tatcgttcaa cgcaaaaatg acaacccgcc cgtcggctcc agacgcctcg
     1201 ggggcggaga gaaacaccgt caggaagcat acaagaaaaa ctgaccgtac cgtactccgc
     1261 atcctctgct tcattacctc acacctcctg tatcatccgt tgcgcctcgt cccacgggac
     1321 gggcggagag aagtagtacc cctgcgcttt cgggcacccc gcgctcttca gccatgcgag
     1381 ctgctccgcc gtctccactc cctccgcgat aatgtcgagc cctagatctc ttcccatggt
     1441 gatgatcgag cgcatcatcc gctccgtctt cgcggaaacg aaaagttggc tgacaaacgc
     1501 cctgtccagc ttgatcacgt cgagcggcag gttgttgata tattggagcg aggagtagcc
     1561 cgttccgaag tcgtcgaggg cgattctgat tccgctctcc ttcagaagat cgagtttgac
     1621 gtgcacatcg tccaggtttt cgatcagcga actctccgtc acctcaaggg tgaagcgcgt
     1681 cggatcggcc cctgtgctct ccaggaaaaa cttcacgctc tccacggcag cggcgctcct
     1741 caggaagacc gacgaggcgt tcgacgagaa gaagagccga agctgcgggt tcagcctgcg
     1801 cgccgtgttg accgcatcaa gagcgtgctc cagcatgcgg gtgtcgaggt ttgcgacgaa
     1861 tccggtctgc tcggcctgcg gtataaaggc cgcgggagga aggatcccct tctgcggatg
     1921 cttccaacgg gagaggg
//
LOCUS       DAOSWW010000537         1936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49342_length_1936_cov_3.907496, whole genome shotgun sequence.
ACCESSION   DAOSWW010000537 DAOSWW010000000
VERSION     DAOSWW010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49342_length_1936_cov_3.907496"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PK442_10145"
     CDS             <1..774
                     /locus_tag="PK442_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOO88056.1"
                     /translation="TLRLYDRLGLLKPQYVDENRYRYYSIKQSARLDMIQNLQLLGMS
                     LRQIREQLDTQDVAVVEKLLEKQKARLDEELLRLSATRRCVERTLINYKRYHSAPRDG
                     MIVVEFIPDRAIYCYDSGINFYDYGLETYEYILRELKGHIALNALPMAYFCNVGTVLR
                     KPLLLRKEFVSTEVFIFVDDDFQQSPNVETLPSGTYLCMSCDSFHKEKEYAGKLLAHA
                     EENGYEIAGDYICEVIADLPVFHNNERGMFIKLQVPILF"
     gene            875..>1936
                     /locus_tag="PK442_10150"
     CDS             875..>1936
                     /locus_tag="PK442_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HOO88057.1"
                     /translation="MERKLAVAQFGCGKMSAYTMRYVYEKGAEIVAAFDMNPAVVGKD
                     IGTIMGTEKKGVTVLDAKMADETLAALKPDACIITTMSLMKDLREAMLVCAKNGVNAI
                     STCEEAFYPWNSSRAVTEEIDELAKKNNCTICGAGYQDVFWGNLIATLAGATHSIRTI
                     RGKSSYNVEDYGIALAQVHGAGLTLDAFEKEIASADNISEAERKALIDKGEFLPSYMW
                     SVNGWLCERLGLTPVSQTQKCVPHTHTSELHSKTLGMTIPAGHATGMSAVVTTETEEG
                     ITLETECIGKVYAPDEFDRNDWTIVGEPDTQVVINRPATVELTCATIVNRLPDIVNAP
                     AGYVTTDKMPTNSYRVKPLH"
BASE COUNT          451 a          587 c          570 g          328 t
ORIGIN      
        1 acgttgcgcc tctacgaccg tctggggctg ctgaaaccgc agtacgtgga cgagaaccgc
       61 tacagatact acagcatcaa gcagtcggcc cgtctggaca tgatacagaa ccttcagctc
      121 ctcggcatgt cgcttcggca gatcagggag cagctcgaca cgcaggacgt cgccgtcgtc
      181 gagaagctgc tcgagaagca gaaggccaga ctcgacgagg aactgcttcg tctctccgcc
      241 accagaagat gcgtcgagcg cacgctgatc aactacaagc gctaccactc cgcccctcgg
      301 gacggcatga tcgtcgtcga attcatcccc gaccgcgcga tctactgcta cgacagcggc
      361 atcaacttct acgactacgg actggagacg tacgagtaca tcctccgtga gttgaaaggg
      421 cacatcgcgc tcaacgctct ccccatggcc tacttctgca acgtagggac cgtgctcaga
      481 aaaccgcttc tgctgaggaa ggagttcgtc tccaccgagg tgttcatctt cgtcgacgac
      541 gacttccagc agtcgccgaa cgtcgaaact ctgccctccg ggacgtatct ctgcatgtcg
      601 tgcgacagct tccacaagga gaaggagtac gccggaaaac tccttgcgca cgccgaggag
      661 aacgggtacg agatcgcggg cgactacatc tgcgaggtga tcgccgatct gccggttttc
      721 cacaacaacg agcgaggcat gttcatcaag ttgcaggttc cgatactttt ttaacgcgca
      781 ccccttgact ctataccatg tagagaccct atagtgaaaa gaagaacacg tggaagaacc
      841 aaaacacact ctacgttcag gggggtattg cgttatggaa aggaaactcg cggtcgccca
      901 gttcggatgc ggaaagatgt ccgcctacac gatgcgctac gtctacgaaa agggcgcgga
      961 gatcgtcgcc gcgttcgata tgaacccggc agtcgtgggc aaggatatcg gtacgatcat
     1021 gggaacggag aagaagggcg tcacggtgct ggacgcgaag atggccgacg aaacgctcgc
     1081 cgcgctgaag cccgacgcgt gcatcatcac gaccatgagc ctgatgaagg atcttcggga
     1141 ggccatgctc gtgtgcgcca agaacggcgt caacgccatc agcacgtgcg aagaggcctt
     1201 ctacccgtgg aattcctccc gcgccgtcac cgaagagatc gacgagctcg cgaaaaagaa
     1261 caactgcacg atttgcggcg cggggtacca ggacgtcttc tggggcaacc tgatcgccac
     1321 gctcgcggga gcgacgcaca gcatccgcac gatacgcggc aagtccagct ataacgtgga
     1381 ggactacggc atcgcgcttg ctcaggtgca cggcgccggg ctgacgctgg acgccttcga
     1441 gaaggagatc gcctctgccg acaacatttc cgaagctgag cgcaaggcgc ttatcgacaa
     1501 gggagaattc cttccctcct atatgtggag cgtcaacggg tggctgtgcg aacgcctggg
     1561 gctgactccc gtttcgcaga cgcagaaatg cgtgccgcac acgcacacgt ccgagctgca
     1621 ctcgaagacc ctcggcatga ccatcccggc aggacacgca accggcatgt cggccgtcgt
     1681 gacgacggag accgaagagg ggatcacgct ggagacggag tgcatcggga aggtgtacgc
     1741 gcccgacgag ttcgaccgga acgactggac gatcgtcggc gaaccggaca cgcaggtggt
     1801 catcaatcgc cccgcgacgg tcgagctgac gtgcgcgacc atcgtcaacc ggctgcccga
     1861 catcgtcaac gctccggccg ggtacgtgac caccgacaag atgccgacca attcctacag
     1921 agtgaagccg ctgcat
//
LOCUS       DAOSWW010000538         1933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49473_length_1933_cov_3.296060, whole genome shotgun sequence.
ACCESSION   DAOSWW010000538 DAOSWW010000000
VERSION     DAOSWW010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49473_length_1933_cov_3.296060"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK442_10155"
     CDS             complement(<1..283)
                     /locus_tag="PK442_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose/sucrose transporter subunit IIB"
                     /protein_id="HOO88058.1"
                     /translation="MEANTVLAGRILELVGGKGNVSSAANCMTRIRMRLKDASKADVA
                     GLKATDGVLGVVEGATLHVIVGPGKAKKVADILVEEFGVARDSAVTEDWE"
     gene            complement(390..1283)
                     /gene="murQ"
                     /locus_tag="PK442_10160"
     CDS             complement(390..1283)
                     /gene="murQ"
                     /locus_tag="PK442_10160"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOO88059.1"
                     /translation="MIELKHLTTEQRNEKTLDLDSMPLDEALRVMNAEDAKVAPAVGE
                     QLPAIAKAVEAVVRSFESGGRLLYLGAGTSGRLGILDATECVPTFGTPPEMVVGRIAG
                     GMGALVRSVEGAEDSLTMGVDDLKELKADSRDTVVGIAASGRTPYVIAALEYAKSVGC
                     ATVGVTCNRNSAVGAAADIAIEIDVGPEVLTGSTRLKAGTAQKLALNMISTMAMVGIG
                     KVYKNLMVDVQRTNEKLRTRAVNIVADAAEVDKETAREFLEKAEGSVKHAIVMLLLGC
                     PLDEARAKLEAAKGRVRLALQ"
     gene            complement(1349..>1933)
                     /locus_tag="PK442_10165"
     CDS             complement(1349..>1933)
                     /locus_tag="PK442_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88060.1"
                     /translation="TRKYGGTGLGLSICSALLQMMGSELRVESVPGEGSRFFFDLRAE
                     RVPVLETAEEERARIVPEEPFMPATRPTILIIEDEELNRRLLRLLLSKLLDADIVEAE
                     GGEEGVERFRERAPDLIFLDLQMPGRDGHWTARTLRELEGAGVHTPIVAFTADVQPAT
                     REACFASGMDDYICKPADLYTLREVLERHLKTKE"
BASE COUNT          274 a          717 c          568 g          374 t
ORIGIN      
        1 cctcccagtc ctcggtcacg gcgctgtcgc gcgcgacgcc gaactcctcg acaagaatgt
       61 ccgcgacctt cttcgccttg ccggggccga cgatgacatg aagcgtcgcc ccctccacca
      121 cgccgagcac cccgtcggtt gccttcagtc cggcgacgtc cgccttcgag gcgtccttca
      181 ggcgcatgcg aatccgcgtc atgcagtttg ccgccgagga gacgttcccc ttgccgccga
      241 caagctccag aatcctgccg gccaataccg tgttcgcttc cattaagtca cacacctctc
      301 ctgttgaaat accgcgctcc gcgtcgttgc gcgaaggccg tcccgctgcg gcggctcttt
      361 gcgcaacgac gcggagctgc gggaaaaact cactgcaggg cgagccgcac gcgccccttc
      421 gccgcctcca gcttcgcccg agcctcgtcg agcgggcagc cgagcagcag catgacgata
      481 gcgtgcttca cgcttccctc cgccttttcg aggaactccc gggcggtttc cttatcgacc
      541 tcggcagcgt cggcgacgat gttgacggcc cgcgtccgga gcttttcgtt cgtccgctgc
      601 acgtcgacca tcaggttttt gtacaccttg cctatgccca ccatcgccat cgtagagatc
      661 atgttgagcg ccagcttctg cgccgtcccc gccttcaacc gcgtggagcc ggtgagcact
      721 tccgggccga cgtcgatctc gatggcgatg tccgccgcag cgcccaccgc ggagtttcta
      781 ttgcatgtga cgcccaccgt cgcgcagccg acgctcttcg cgtactccag cgcggcgatc
      841 acgtacggcg tgcgtccgct ggccgctatc cccaccacgg tgtccctgga gtcggccttg
      901 agttccttca ggtcgtccac acccatggtc aggctgtcct ccgcgccctc cacggagcgc
      961 accagcgctc ccatgccgcc cgcgatgcgt cccacgacca tctccggcgg cgtgccgaag
     1021 gtgggaacgc actccgtcgc gtcgaggatt cccaggcgtc cgctggtccc cgcgcccagg
     1081 tagagcagtc gccccccgct ctcgaaagag cggaccacag cctccaccgc cttcgcaatg
     1141 gcggggagct gttcccccac tgcgggggcg actttcgcgt cctccgcgtt catcacccgg
     1201 agggcctcgt cgaggggcat gctgtccagg tcgagcgtct tttcgttgcg ctgttccgtc
     1261 gtcaggtgct tcagttcgat catgcgcgct tcttcctttc gttgtgtgcg tcgtcgcggc
     1321 ggtctccggc ggggcgccgg atgtcccgct actctttcgt cttcagatgc cgctccagca
     1381 cctcgcggag cgtgtagagg tcggcgggtt tgcagatgta gtcgtccatg ccgctcgcga
     1441 aacaggcctc tctcgtcgcg ggctgcacgt cggctgtgaa ggcgacgatc ggcgtatgca
     1501 cccccgcccc ctccagctcc cgcagcgtcc gcgccgtcca gtggccgtca cgtccgggca
     1561 tctggagatc aaggaagatc aggtcgggag cgcgctcgcg aaagcgttcg acaccctctt
     1621 cgcccccctc cgcctcgacg atgtcggcgt cgagcagctt ggagagcagc aggcgcaaca
     1681 gccgccggtt cagctcctcg tcctctatga tcagtatggt cggtctcgtc gccggcatga
     1741 agggctcctc cggaacgatc cgcgctctct cttcctccgc cgtctccaaa accgggacgc
     1801 gctccgcccg caggtcgaag aaaaagcggc tcccctcgcc cgggacgctc tcgaccctga
     1861 gttcgctccc catcatctgg aggagcgcgc tgcagatgga gaggccgagt ccggtgccgc
     1921 cgtactttcg cgt
//
LOCUS       DAOSWW010000539         1931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49558_length_1931_cov_3.754264, whole genome shotgun sequence.
ACCESSION   DAOSWW010000539 DAOSWW010000000
VERSION     DAOSWW010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49558_length_1931_cov_3.754264"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1248
                     /locus_tag="PK442_10170"
     CDS             241..1248
                     /locus_tag="PK442_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88061.1"
                     /translation="MNLKRIVVCALMTALVASCAAGTAFAADTVPLEGRMTLYTSQPE
                     RDVTKLIAAFNELHPDVAVDVFRSGTEEVVSKVRAEHAVRSVLADVLLVADSVTFESL
                     KEDGMLLPYDSPEMKGIPEEYLDKDRTYAGTKIISTGIIYNTTKVKEPPVSFADLAKS
                     EYKDSIIMPSPLYSGAAAYNLGVMTRTEGLGWEFYEALKANNIRIEKGNGAILTAVVA
                     GEKACGLVVDFMTVRSKRDGAPVEFVYPLEGSPAVTEPIGILKTTEVPELAKAFVDFV
                     LSAEGQKVAAEMGYTPVKDGVEAPEGLKSVGEIKSMVYDIAVLKEAREAEKARFSAMF
                     Q"
     gene            1264..>1931
                     /locus_tag="PK442_10175"
     CDS             1264..>1931
                     /locus_tag="PK442_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO88062.1"
                     /translation="MRVRQFAHTFGSRALKTAFYGAVFLFFIAPLLRLLLMSFAGEAG
                     YDASHYVRLLAEERTRLAIRNTAIIGTSATAFSVLFGGGFAFLVAYANLKRRRLAEVL
                     ILLPFIIPSYIITLSWTGLLSGNGAINTWLTSAGLPAVNLYSMGGIIFVHGMCATPIV
                     YMIVKAMLRRIPVDLEWASMAAGYTRRQTMLRINLALAAPAVASGAMLSFLATIDNFS
                     IPAFL"
BASE COUNT          361 a          646 c          599 g          325 t
ORIGIN      
        1 gagaacataa gacgacgtcc gtccccgcgg acgggctcgc gcttcgccgc gccgccggaa
       61 cgatttttcc gccgcaaagc agtataatgt tgctttgcgg ttacacccgc gcaacgcttt
      121 tgttacgcgt gcgccgtaca ctctgcggga acgccttcgc ctcagcccga cggcgccggt
      181 cggaaggcaa ggcgcggaac acccgcaaaa cgcggactca cacgcaagga ggagaactga
      241 atgaatctga aacgaatcgt agtctgcgcg ctgatgacgg ccctcgtcgc atcgtgcgcc
      301 gccggaacgg ctttcgcggc ggatacggtc ccgctcgaag ggcggatgac cctgtacacg
      361 tcccagccgg aacgggacgt gaccaagctc atcgcggcct tcaacgagct gcatccggac
      421 gtcgctgtgg acgtcttccg ctcgggaacc gaggaggtcg tcagcaaggt ccgcgcggag
      481 cacgccgtgc ggtcggtgct cgcggacgtg ctgcttgtgg ccgacagcgt caccttcgag
      541 agcctcaagg aggacggcat gctcctcccc tacgactccc ccgagatgaa gggcatcccc
      601 gaggagtatc tggacaaaga ccgcacctac gcgggaacga aaatcatcag taccggcatc
      661 atctacaaca ccacgaaggt gaaagaacct cctgtctcct tcgccgacct ggcgaagagc
      721 gaatacaagg acagcatcat catgcccagc ccgctctact ccggcgccgc cgcgtacaac
      781 ctcggcgtga tgacccggac cgaggggctc ggctgggagt tctacgaggc gctgaaggcg
      841 aacaacatcc gcatcgagaa ggggaacggc gccatcctca ccgcggtggt cgcgggcgag
      901 aaggcctgcg gactcgtggt cgacttcatg accgtccgct cgaaacgcga cggcgcgccg
      961 gtggagttcg tctacccgct tgaaggctcc cctgcggtca ccgagccgat aggcatcttg
     1021 aagacgacgg aagttcccga gctggcgaag gccttcgtcg acttcgtcct ctcggccgag
     1081 gggcagaagg tggcggcgga gatggggtac acgcccgtca aggacggcgt cgaggccccc
     1141 gaagggctga aatcggtggg cgagatcaag agcatggtct acgacatcgc cgtgctgaaa
     1201 gaggcgcgag aggccgaaaa ggcgcgattc agcgctatgt tccagtagaa accggggaga
     1261 acggtgcgcg tacggcaatt tgcacacact ttcggaagcc gcgccctgaa gacagccttc
     1321 tacggcgcgg tcttcctctt tttcatcgcg ccgctgctcc gcctgctgct gatgagcttc
     1381 gccggggagg cagggtacga cgcgtcgcac tacgtccgcc tcctcgccga ggagcggacg
     1441 cggctggcca tcaggaacac ggcgatcatc gggacgagcg ccacggcgtt ttccgtactc
     1501 ttcggcgggg gattcgcgtt cctcgtcgcc tacgcgaacc tgaagcggcg gcgtctcgcc
     1561 gaggtgctga tcctgctccc cttcatcatc ccctcgtaca tcatcacgct ctcctggacg
     1621 gggctgctct cggggaacgg cgcgatcaac acgtggctga cctccgccgg gctccccgcc
     1681 gtcaacctct actccatggg cggcatcatc ttcgtccacg gcatgtgcgc gacgcccatc
     1741 gtctacatga tcgtgaaagc catgctgcgg cgcatccccg tcgaccttga atgggcctcg
     1801 atggccgcgg ggtacacgcg ccgccagacc atgctccgga tcaacctcgc gctcgccgcg
     1861 cccgccgtgg cgagcggcgc gatgctctcc ttcctggcca ccatcgacaa cttctccatt
     1921 ccggccttcc t
//
LOCUS       DAOSWW010000540         1930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49614_length_1930_cov_3.172267, whole genome shotgun sequence.
ACCESSION   DAOSWW010000540 DAOSWW010000000
VERSION     DAOSWW010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49614_length_1930_cov_3.172267"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PK442_10180"
     CDS             complement(<1..597)
                     /locus_tag="PK442_10180"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88063.1"
                     /translation="MMLSAFVLFFAAVLLGMPIAIALGLTGILIAWLSGIFNPVLIGH
                     VMYQGVDSFTLLAVPFFMLAGALMEEGGISRRLMNFSFALVGRMRGGLAQVAVVLSML
                     FAGLSGSAVADTAAVGSLLIPSMKEKGYREGFAAALVAAGGAIGPIIPPSIPMVIFAV
                     IANVSVAELFLGGIFPGVFFGLVLMAYSYVEAKRYDYPR"
     gene            complement(594..1091)
                     /locus_tag="PK442_10185"
     CDS             complement(594..1091)
                     /locus_tag="PK442_10185"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO88064.1"
                     /translation="MKKISALVSAAAAYIDKAATWLLFLLLFVMTGITFAQVVTRYVF
                     NFSITWSEELARFLFIWSIFAGIPSLIFRSGMTAFDLILARLKGKIAKCAVLLLSGAG
                     LLFFHLMAYGSFPLVQRQFAQQATALPVPMGLVYIVIPVSGFTAMFMIVENLLRVLSE
                     RGEAA"
     gene            complement(1168..>1930)
                     /locus_tag="PK442_10190"
     CDS             complement(1168..>1930)
                     /locus_tag="PK442_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO88065.1"
                     /translation="AEGLQLGTVEMGTQSPGGLASFLPLMGVLELPYIYRSNEHVYKT
                     VDGPIGLELNEKIRAKTGIRILGYWMNLYRNTTNSKHPVVLPDDFKGLKIRVPETKTV
                     MDTIAALGGNPVPMVFGEVYTALQQGIIDGQENPLSIIWASRLYEVQKYLSLTGHVYS
                     PVVIMISDAFYNSLPEDLQKIVVEAFEEVRPVARKMVEDQETELVEQLREKGMEVNTV
                     EPEAFQAKMGGVYDDFVKNVGKEAQEFIDRIKEVR"
BASE COUNT          430 a          563 c          577 g          360 t
ORIGIN      
        1 acgggggtag tcgtacctct tggcttcgac gtaggagtag gccatcagga cgaggccgaa
       61 aaagacgccg ggaaagatgc cacccagaaa cagctcggcc acggagacgt tggcgatgac
      121 ggcgaagatg accatgggga tgctcggcgg gatgatcggg ccgattgcgc cccctgcggc
      181 gacaagggcc gccgcgaacc cctcccggta ccctttttcc ttcatcgagg ggatgagcag
      241 cgaaccgact gctgcggtgt cggcgactgc cgacccggag agcccggcga agagcatcga
      301 aagcacaacg gcgacctgcg cgaggccgcc ccgcatccgt ccgacgagcg cgaaggagaa
      361 gttcatcagg cgtctggaga ttcctccctc ctccatgagc gctccggcga gcatgaagaa
      421 cggtacggcg agcagggtga agctgtccac gccctggtac atgacgtggc cgataagaac
      481 agggttgaaa ataccggaaa gccatgcgat gaggataccc gtcagcccga gagcgatcgc
      541 gatcggcatt ccaagcagaa cggcggcgaa aaagagaacg aatgcggaga gcatcatgcg
      601 gcttcccccc tttccgagag cacccgcaga aggttctcga cgatcatgaa catcgccgtg
      661 aagccgctta ccggaatcac gatgtacacg agccccatcg ggacggggag agcggtcgcc
      721 tgctgagcga actggcgctg cacaagcggg aagctgccat acgccatcag gtggaaaaag
      781 agaagccccg cgcctgagag cagcagcacg gcgcacttgg cgatctttcc tttaagacgg
      841 gcgagaatga ggtcgaacgc ggtcatcccg gaacggaaga tcagcgacgg gatgcccgcg
      901 aagatgctcc aaatgaagag aaagcgcgcc agctcttcgc tccacgtgat cgaaaagtta
      961 aagacgtacc gggtaaccac ctgcgcaaaa gtgatccccg tcattacgaa cagaagcaaa
     1021 aaaagaagcc acgttgcggc cttgtcgatg tacgcggccg cggcggagac aagagcgctg
     1081 atttttttca tccgtatttc tccttcgggt ctataaaaag actccggccc cctctgcgcg
     1141 cattgagggc cggagtagct cgggaaacta ccggacttcc ttgattcggt cgatgaactc
     1201 ctgcgcctct ttcccgacgt tctttacgaa gtcgtcgtac acgccgccca tcttcgcctg
     1261 gaatgcctca ggctcgacag tgttgacctc catccccttc tctctcagct gctcaaccag
     1321 ttcggtctcc tgatcctcga ccatttttct ggcgaccggc ctgacttcct cgaaggcctc
     1381 gacaacgatc ttctgaagat cttcagggag agagttgtag aaggcgtcgg agatcatgat
     1441 gaccacggga gagtacacgt gtccggtaag agagagatac ttctgcacct cgtagagacg
     1501 agaggcccag atgatggaga gcggattctc ctggccgtcg atgatcccct gctgcagcgc
     1561 cgtgtagacc tcgccgaaga ccatgggtac ggggtttccg cccagcgcgg caatggtgtc
     1621 catgactgtc ttcgtctcgg ggacgcggat tttgagcccc ttgaagtcgt ccggaagcac
     1681 gaccgggtgt ttggagttgg tggtgttccg gtagagattc atccaatacc cgagaatgcg
     1741 tatacccgtc ttcgcacgga ttttttcgtt gagttcgagg ccgatcggtc cgtcgaccgt
     1801 cttgtacacg tgttcgttgc tcctgtaaat atagggcagc tccagcacgc ccatgagcgg
     1861 aaggaacgat gccagacctc ccggactctg ggttcccatt tcgaccgtcc cgagctgcaa
     1921 cccttcggcg
//
LOCUS       DAOSWW010000541         1924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49863_length_1924_cov_3.716426, whole genome shotgun sequence.
ACCESSION   DAOSWW010000541 DAOSWW010000000
VERSION     DAOSWW010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49863_length_1924_cov_3.716426"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK442_10195"
     CDS             <1..220
                     /locus_tag="PK442_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO88066.1"
                     /translation="AGCLGVALFQTINVTLPRLGATAMVTLIIVGQLLTGVLIDAQGW
                     LGTAARPFDMFRAAGVLLLLAGGWLIVR"
     gene            282..1487
                     /locus_tag="PK442_10200"
     CDS             282..1487
                     /locus_tag="PK442_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOO88067.1"
                     /translation="MADFRASAQQLHQQVTTWRRDLHRIPETGVDTPQTEAYICAELD
                     KIGVSYRKGLGRHGVVALVEGKHKNKVFAIRADMDGLPIKEETGLPFASQNGCMHACG
                     HDAHAAMGLGSVKLLHDVRDQIEGSVLVVFQPGEEGCTDGPGGAKRMLDDGAFDDPKP
                     DAMVGLHTGSIWKDFVPGEIGYRPGGIMACMDRFDILIKGKGAHGANPQGSVDPISIA
                     GQIICELQTIISREVDPQEPCVISLGEIHAGSAFNIIPGECRITGTVRAFNQEMREFL
                     ARRIGEVASAVASGMRGSVEFSYGWHGPSPVVNDPVMTEELRLAAEKIVGPEKVREIP
                     RPSMGGEDVAFFLEKAPGTFFFLPGCNEAKGQTWPHHNSRFDIDEDVLWIGPAVMATM
                     AFDWLKKQA"
     gene            complement(1531..>1924)
                     /locus_tag="PK442_10205"
     CDS             complement(1531..>1924)
                     /locus_tag="PK442_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOO88068.1"
                     /translation="ELWGKKGGGFFYWQQLYHAFYCVDYFLLPAGSEFPEKPYGSAVA
                     MFRETLDTVPAKDEIREYGAMMKAKADAWIAALDDGMLGDRHEGMSERRKTEVTNAGV
                     LTSLCGHNMYHVGCLDSVLRENGLKGVY"
BASE COUNT          369 a          591 c          622 g          342 t
ORIGIN      
        1 cgcgggatgc ctgggagtgg cgctgttcca gacgatcaac gtaacgctcc cccgcctggg
       61 cgcgacggcc atggtgacgc tgatcatcgt aggacagctg ttgacgggcg tcctcatcga
      121 cgcgcagggg tggctgggta cggcggcgcg cccgttcgac atgttcaggg cggcgggcgt
      181 gctcctgctg ctcgccggag gctggctgat cgtacggtag gcgtttttct gcgttgtttt
      241 ttacgggaca tcaacgaaca aagagcaaag gagatggtgt aatggcggac tttcgcgcat
      301 cagcgcagca gcttcatcag caggtaacaa catggcggcg ggatctgcac agaatccccg
      361 agacgggagt ggacacaccg cagacggagg cgtatatctg cgccgagctg gataaaatag
      421 gggtctccta ccgcaagggg ctcggcaggc acggcgtcgt cgcgctcgtc gagggaaagc
      481 ataaaaacaa ggtcttcgcg atccgcgcgg acatggacgg tcttcccatc aaggaagaga
      541 cggggcttcc cttcgcctcg cagaacggct gtatgcacgc atgcgggcac gacgcgcacg
      601 cggcgatggg actgggctcg gtgaagctgc tgcacgacgt cagggaccag atcgagggct
      661 cggtactcgt cgtcttccag cccggcgaag aggggtgcac cgacggcccc ggcggagcaa
      721 agcggatgct cgacgacgga gcgttcgacg atccgaagcc ggacgcgatg gtcgggctgc
      781 ataccggctc catctggaag gattttgtcc ccggtgagat cggctaccgc cctggaggaa
      841 tcatggcctg catggaccgc ttcgatattc tgatcaaagg gaaaggggcg catggagcaa
      901 accctcaagg ctccgtagat cccatctcta tcgcagggca gatcatctgc gagttgcaga
      961 cgatcatcag ccgcgaggtc gaccctcagg agccgtgcgt gatcagcctg ggcgagatcc
     1021 acgcgggaag cgccttcaac atcatccccg gagagtgccg catcaccggc acggtccggg
     1081 ccttcaatca ggagatgcgg gagttccttg cccggcgaat cggcgaggtg gcctcggcgg
     1141 tggcctccgg aatgcggggg agcgtcgagt tctcgtacgg ctggcacggc ccctcgcccg
     1201 tggtgaacga ccccgtcatg acggaggagc ttcgcctggc ggcggaaaag atcgtcggtc
     1261 ccgagaaagt ccgggagatc ccccgcccct cgatgggcgg cgaggacgtc gccttcttcc
     1321 tcgaaaaggc ccccggcacg ttcttcttcc tccccggctg caacgaggcg aaggggcaga
     1381 cgtggccgca ccacaacagc cgtttcgaca tcgacgagga cgtcctctgg atcggaccgg
     1441 ccgtcatggc cacgatggcc ttcgactggc tgaagaaaca ggcgtagttt tcaaagaagg
     1501 gagtcccgca acggactccc ttccgccctc ttaatatacg cccttcagcc cgttctcccg
     1561 cagcacagaa tcaaggcagc caacatggta catgttgtgg ccgcacagcg acgtaaggac
     1621 acccgcgttg gtcacttcgg tcttccgtcg ctcggacatg ccttcgtgcc tgtcgccgag
     1681 catgccgtcg tcgagagcgg cgatccatgc gtccgccttc gccttcatca tcgcgccgta
     1741 ctcgcgaatt tcatccttcg ccgggacggt gtcgagggtc tcgcggaaca tggccaccgc
     1801 gctgccgtac ggcttctccg gaaattcgct tcctgcgggg agcaggaagt agtccacgca
     1861 gtagaacgca tgatacagct gctgccagta gaagaagccg ccgcctttct tcccccagag
     1921 ttcg
//
LOCUS       DAOSWW010000542         1923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49905_length_1923_cov_3.042827, whole genome shotgun sequence.
ACCESSION   DAOSWW010000542 DAOSWW010000000
VERSION     DAOSWW010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49905_length_1923_cov_3.042827"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PK442_10210"
     CDS             <1..328
                     /locus_tag="PK442_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO88069.1"
                     /translation="PTALFAFNDLMAFGAITALAERGVRIPEDVSVVGYDDILLSSFF
                     NPPLTTVAQPKKEMGRAAFGLLLERINDESLPARSLRMNARLVVRKSTASPGASKGGD
                     GSGCMR"
     gene            468..1505
                     /locus_tag="PK442_10215"
     CDS             468..1505
                     /locus_tag="PK442_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO88070.1"
                     /translation="MKGLRKVLLLVMVLVIALSAFGVAGAAESKVKVALVLSGFLGDK
                     SFNDSAYEGLERSIKEFGIDLKVLESKVPSDWESNLVAMAAEGYDLVLGCSTQLQEII
                     AKHAPDFPDVKFGIIDGVVKAPNVMSAIFAQNEGSFLAGAAAAMFTTKTDVPGVNAEK
                     IIGWVGGMDIPVLHDFLVGYKQGAAYVDPETRVLVSFAGTFNDPLKGKELTLAQFEQG
                     ADIVMNVASNTGNGILEAAYEAKKYAIGVDLNQDEAYPGHILTSMLKRVDMGSYLIVK
                     AVVEGNFEGGKILDMTIANGGVGLTDMSTMKKALGDKFPQDILESIAKLTEEVKGGKI
                     KVDSYEGFQRN"
     gene            1568..>1923
                     /locus_tag="PK442_10220"
     CDS             1568..>1923
                     /locus_tag="PK442_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88071.1"
                     /translation="MSRILIEMKGICKEFPGVKAVQDGLFSLEEGEIHSLIGENGAGK
                     STLMKILYGMYVPEEGSIAVKGETYPSLTPKQAIGLGIGMVHQEFMLVREMTVLENII
                     LGFEPRRGGRIDRRAAL"
BASE COUNT          384 a          555 c          619 g          365 t
ORIGIN      
        1 cccgacagca cttttcgcct tcaacgacct gatggccttc ggcgcgatca ccgccctcgc
       61 ggagaggggg gttcgcattc cggaggacgt ctccgtcgtc ggctacgacg acatactgct
      121 ctcctccttc ttcaatccgc cgctgacgac cgtggcgcag ccgaagaagg agatggggag
      181 ggcggctttc ggtctcctcc tcgaaaggat caacgacgag tcgctgcccg cgcgctctct
      241 gcgtatgaac gcgcggctcg tggtgcgaaa atccaccgct tcgccgggtg cgtcgaaagg
      301 aggggatggg tcgggctgta tgcgatgaac cgctccgggg gagggttgct tttcccgcgg
      361 ggcgcgatgc ggcacggtgc gctcctggtt ttccggacga attccgtccg gtcggtccgt
      421 ccttcgaggg gcggaaacat actatccaca ttcagggagg gaaacgcatg aaaggtttga
      481 gaaaagttct tctgctggtt atggttctgg ttattgcgct ctccgcgttc ggcgtcgccg
      541 gagcggccga gtcgaaagtc aaggttgctc tggtcctctc cggcttcctg ggcgacaagt
      601 ccttcaacga ttccgcgtac gaggggctcg agcgctcgat caaggaattc ggcatcgatc
      661 tgaaggtcct tgagtccaag gtcccctccg actgggagtc caacctcgtc gccatggcgg
      721 ccgaggggta cgacctggtt ctgggttgca gcacgcagct tcaggagatc atcgccaagc
      781 atgctcccga cttccccgat gtgaagttcg gcatcatcga cggcgtggtg aaggctccga
      841 atgtcatgtc cgccatcttc gcccagaacg agggctcctt cctcgccgga gctgccgcgg
      901 ccatgtttac aacaaagact gacgttcccg gcgtcaacgc cgagaagatt atcggctggg
      961 tcggcggcat ggacattccg gtgcttcacg acttcctcgt gggctataag cagggcgctg
     1021 cctacgtcga tcccgagacc cgcgtgctcg tctccttcgc cggaacgttc aacgaccccc
     1081 tgaagggcaa ggagctgacg ctggcccagt tcgagcaggg cgcggacatc gtcatgaacg
     1141 tcgcgtcgaa caccggcaac ggcatcctcg aggcggccta cgaggcgaag aagtacgcca
     1201 tcggcgtcga cctgaatcag gacgaagcct accccggaca catcctcacc tccatgctca
     1261 agagagtgga catgggcagc tacctgatcg tgaaagcggt cgtcgagggc aacttcgagg
     1321 gcggcaagat tctcgacatg accatcgcca acggcggcgt gggactcacc gacatgtcca
     1381 cgatgaagaa ggctttgggc gacaagttcc cgcaggacat cctggagagt atcgcgaagc
     1441 tgaccgaaga agtcaagggc ggcaagatca aggtcgactc ctacgaggga ttccagagaa
     1501 attagacatc attttccgga ggcggcgggg agatttcccc gtccgtctcc attgcgaggt
     1561 ggggttcgtg tcgcgtatcc tgatcgaaat gaaaggcatc tgcaaggaat tccccggagt
     1621 gaaggcggtc caggacggac tgttctcgct cgaggaggga gagatccact cgctcatagg
     1681 agagaacgga gcggggaagt cgacgctcat gaagattctc tacggcatgt acgttcccga
     1741 ggaggggagc atcgccgtca agggagaaac gtatccgtcg ctgacgccga aacaggcgat
     1801 cgggctaggg atcggcatgg tgcaccagga gttcatgctc gtccgcgaga tgacggtgct
     1861 ggagaacatc atcctcggct ttgaaccgag gcggggaggg cgcatcgacc ggagggcggc
     1921 gct
//
LOCUS       DAOSWW010000543         1922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49950_length_1922_cov_2.591323, whole genome shotgun sequence.
ACCESSION   DAOSWW010000543 DAOSWW010000000
VERSION     DAOSWW010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49950_length_1922_cov_2.591323"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PK442_10225"
     CDS             <1..371
                     /locus_tag="PK442_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOO88072.1"
                     /translation="ISSFGFKHGAPADCDYVFDVRFLDNPFYNPVLKPLSGKDEPVQE
                     EIWRSVEARGFWEKLISFLEYIVPLYLRSGKAHLHIGVGCTGGRHRSVAVAERLYAHF
                     KEYSGGCILRHRDIEKDQGW"
     gene            374..1495
                     /gene="yvcK"
                     /locus_tag="PK442_10230"
     CDS             374..1495
                     /gene="yvcK"
                     /locus_tag="PK442_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOO88073.1"
                     /translation="MDLLISYGLGLFTAIILVFLFRLRYSGRTGQLLQRAPQERSTAI
                     SKAIEYRLSMGPCVAALGGGTGLSTLLRGLKSFTRNIVAVVTVTDEGGSSGKLREEWG
                     VLPPGDVRNCIVALAENDNALKRLLDFRFDRGALAGHSLGNLMLLAISEQFGDFRLAV
                     EEMNHLLAIRGRVLPVTTESVSIVAETRDGSRIRGELSVSEHGSQIHKIWLEPRDAKP
                     LKEVLLSLDEADIIVLGPGSLFTSIIPNLLIRKVAERLRQSEAPKVYIANLMTQPQET
                     QGFNILDHVRWISAAMGVSPDYVLVNEGVIPHLLEEKYRVEGAEPLYITEEEEWELEK
                     MGCKILRGDFVRIMDGNVVRHNGQALSEILIRLCRELDE"
BASE COUNT          445 a          476 c          516 g          485 t
ORIGIN      
        1 tgatctcatc cttcggtttc aaacacggag ctcctgctga ttgcgactac gttttcgacg
       61 ttcgtttcct ggataatccc ttctacaatc cggttctcaa accgctttcg gggaaagacg
      121 aacctgttca agaggagatc tggcgttcag ttgaggctcg cggtttttgg gagaagctca
      181 tttcttttct cgaatatatc gtccctctgt atctccgttc agggaaagcg catttacata
      241 taggcgtcgg ctgcacagga ggacgacatc gctcagtggc cgttgcggaa cgtctctacg
      301 ctcatttcaa agagtattca ggcggatgca ttcttcgtca ccgggatatt gaaaaagatc
      361 aggggtggtg attatggacc tgttgataag ctatggactc ggtcttttta ccgccataat
      421 cctcgtcttt ctttttcgtt tgagatactc cgggcgtacc ggacagcttc ttcagcgagc
      481 gccgcaagag cgcagcacag ccatctcgaa agctatcgag tatcgccttt cgatgggacc
      541 ttgtgttgcg gcgctcggcg gaggtaccgg gctttcaacc cttctgagag ggctaaaaag
      601 ctttacacgc aatatcgtgg cggttgttac agtaactgac gaaggaggca gttcgggaaa
      661 actccgcgaa gagtggggag tcctccctcc gggcgacgtc cgtaactgca tagttgctct
      721 tgcagaaaac gacaatgcgc tgaagcgcct tctcgacttt cgatttgatc gcggcgcatt
      781 ggcggggcat agcctcggca acctgatgct gctcgctatc tccgagcagt tcggcgactt
      841 ccgtctcgcg gtagaagaaa tgaatcatct gctcgctatt cgcggcagag tcctcccggt
      901 aactacggaa tccgtaagta tcgtggctga aacacgggac ggttcgcgca tccgagggga
      961 gctctctgtg tccgaacatg gatctcagat tcacaaaatc tggcttgaac caagagacgc
     1021 caagccgctc aaggaagtgc tgctttcgct tgatgaggcc gacatcatcg ttctgggtcc
     1081 gggaagcctc tttaccagta tcattccgaa cctcctcatt cgaaaggttg cagaaaggct
     1141 gcgccaaagt gaagccccaa aagtctatat tgcaaatctc atgacgcaac ctcaggaaac
     1201 ccaaggattc aacatacttg atcacgtccg ctggatcagc gcggccatgg gagtatctcc
     1261 ggattacgtc ctcgtgaacg agggtgtgat tcctcatctt ctcgaagaaa aataccgtgt
     1321 agagggtgcg gaacctcttt atattactga agaagaggag tgggagctgg aaaaaatggg
     1381 gtgcaagata ctacggggag acttcgttcg aatcatggac ggtaatgtag tgcgacacaa
     1441 cggacaagcg ctctccgaga ttctgatacg actgtgccgg gaactggatg agtgatgaaa
     1501 ccgatagatt cccgctcaac gctgacgtca agcatgtggg acgaatggac atcgtcaata
     1561 gcctcccccg gtcgtgctgt cgaggatgaa atcagaggga tagtgtccgg tctttcagag
     1621 gtggcatctc atgaaaggga aactgttttt tcgagtggac gcctttggat ttttcgacgc
     1681 ctcagaatgc tctggtctgc atgggaaaac gcacggaatt atgaattcgc ttcgctcctc
     1741 aacatcccgc gttcaatgaa aggacgcgtt tttttccgtc ttccttcaga aatttatgcg
     1801 acgttccgcg aacgagaatc tgaatctcga aatacgccct ggctgagggg gttgtgggga
     1861 agttgcggct ctttgtattt gccgaagaca ggctattatc tctgttttcg aatctcctcc
     1921 cg
//
LOCUS       DAOSWW010000544         1921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_49977_length_1921_cov_3.428189, whole genome shotgun sequence.
ACCESSION   DAOSWW010000544 DAOSWW010000000
VERSION     DAOSWW010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49977_length_1921_cov_3.428189"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..753
                     /gene="fliS"
                     /locus_tag="PK442_10235"
     CDS             265..753
                     /gene="fliS"
                     /locus_tag="PK442_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOO88074.1"
                     /translation="MDSKNAQNAQTVYLANKVNTASREQLLLITYDIGIKSCKGAENA
                     LDAGVFDEANKHIQKAQDVLRELMVTLRVEEGGEVAQNLMRLYDFMYLMLVEANVSKD
                     REKISVVRGMLEELRTTWEEAIVKLAEEAGIEKPMPHLQGGAAAKEEARAVPVGGLNI
                     AG"
     gene            743..1198
                     /gene="flgN"
                     /locus_tag="PK442_10240"
     CDS             743..1198
                     /gene="flgN"
                     /locus_tag="PK442_10240"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOO88075.1"
                     /translation="MQADLEKKLSSLVSSELALFNELALLVEKEEECVLQEDMECLLN
                     VLQEKQDVISRQEKIHEGWSFISSSLGLSEGRDGPSFWSRLGELLGEGADDLKASLSV
                     IRDVAGNVLEQEIRVQGLLEKHVDSLRSQMAQVSRGKKALQGYSKAGGV"
     gene            1195..>1921
                     /locus_tag="PK442_10245"
     CDS             1195..>1921
                     /locus_tag="PK442_10245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOO88076.1"
                     /translation="MRGSLAMKFNAVRKLLFPVCCLLLLAASSLFADELTKEIALGKK
                     VAAEVEEKWELVTDPARTARLGMLLTRLLPHTTRPLPFEARIVREDMINAFSLPGGIV
                     YFTTGMLDFLRTDAEIAAILAHELVHADRRHVMIQTARSSKISIAALALMIATQGAAG
                     PMILTSLAQVAVTNSYSKDLEREADKEGFRMLVAAGFPPAAMVTSLEAMIFDQLKHPY
                     VDPGVFMTHPELSERVEYILKMSE"
BASE COUNT          434 a          488 c          553 g          446 t
ORIGIN      
        1 ttcctgcaat ggttgaactc tcctccggca cagtgaccag gaaggcttcc gttgccgccg
       61 cttcggatgc cgcatctttt ttaaagtagt ttttctcaaa gggggtcgtg tcatgcagca
      121 ggatgcggct ccctcttttt cttcgcccgg cctggagtcg ggcaggaaag attcggtgcg
      181 tactttcaaa atgaccgctg ttcctggtaa ggttttctct ctcctttacc gaaacaagat
      241 ggagagaaag gagtgatctc cgcaatggat tcgaaaaacg cacagaacgc ccagacagtc
      301 taccttgcca ataaagtgaa cacagcctct cgcgagcagc ttcttctcat aacatacgat
      361 atcggcatca agagctgcaa gggagctgag aacgccttgg atgccggtgt tttcgacgag
      421 gcgaataaac atattcaaaa agctcaggac gttcttcgtg agcttatggt gacgctccgt
      481 gtcgaagagg gcggcgaagt tgcgcagaat ctgatgcgcc tttacgactt catgtatctc
      541 atgcttgtcg aggcaaacgt gagcaaggac cgcgaaaaga tctcagtcgt cagaggaatg
      601 ctcgaagagc ttcggaccac atgggaggaa gctattgtga agcttgcgga agaggcgggt
      661 attgagaagc ctatgcctca tcttcaggga ggcgccgctg cgaaagagga ggctcgcgct
      721 gtcccggttg gagggttgaa tattgcaggc tgatctcgaa aaaaaactct ccagccttgt
      781 ttcctccgag ctcgcgctct tcaacgagct cgctcttctt gtggagaaag aagaggagtg
      841 cgttctccag gaggatatgg aatgtctttt gaacgtgctg caggaaaagc aggacgttat
      901 ttcccgtcag gaaaaaatcc acgaggggtg gagcttcatc tcttcgtcgc tcggactctc
      961 cgaagggaga gacggccctt cattctggag ccgcctggga gaacttctcg gcgagggcgc
     1021 ggatgatttg aaagcctctc tttctgttat ccgggatgtc gccggaaatg ttctggaaca
     1081 ggaaatccgc gtccaggggc ttctggaaaa gcatgtcgat tccctccgaa gccagatggc
     1141 ccaggtgagc cgggggaaaa aagcgctgca aggctattca aaagcaggag gagtatgaga
     1201 ggttcgcttg ccatgaaatt caatgctgtt cgcaaactgc tctttccggt ctgttgcctg
     1261 cttcttctgg cagcttcgtc tctttttgcc gacgaattga caaaagagat tgctctcggg
     1321 aagaaagtcg cggcggaagt tgaagaaaaa tgggagcttg taacggatcc cgcgcggacc
     1381 gcgcgtctcg gaatgctcct cacccggttg cttccgcaca cgacgagacc tcttcccttc
     1441 gaagctcgta ttgtacggga ggacatgatc aatgcgttca gccttcccgg agggattgtc
     1501 tatttcacca cggggatgct ggactttttg cggacagacg cggaaattgc ggcaatcctg
     1561 gctcatgagc tcgtccatgc ggacaggcgg cacgtgatga tccagaccgc ccgttcgtcg
     1621 aagatttcta tcgccgcttt ggcgttgatg atcgccaccc aaggcgccgc aggaccgatg
     1681 atcctgacca gccttgcgca ggttgcggtg acaaactcct acagcaagga tctggaacgg
     1741 gaggccgaca aagaggggtt ccgtatgctc gtcgccgcgg gctttcctcc cgcagcgatg
     1801 gtcacctcgc ttgaggccat gatcttcgac cagctcaagc atccgtacgt ggatcccggt
     1861 gtatttatga cccaccccga gctttcggag agggttgagt atattctgaa gatgtcggaa
     1921 g
//
LOCUS       DAOSWW010000545         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50015_length_1920_cov_3.052547, whole genome shotgun sequence.
ACCESSION   DAOSWW010000545 DAOSWW010000000
VERSION     DAOSWW010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50015_length_1920_cov_3.052547"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..557)
                     /locus_tag="PK442_10250"
     CDS             complement(333..557)
                     /locus_tag="PK442_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOO88077.1"
                     /translation="MRSRSWKLRIRDIVDAVRTILGYIEGMTFDDFVRDQRNMDAVVR
                     RLTIIGEAAARVPEFVCENIGLSPGPKCAR"
     gene            complement(547..846)
                     /locus_tag="PK442_10255"
     CDS             complement(547..846)
                     /locus_tag="PK442_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO88078.1"
                     /translation="MTQLERTVSILETNKELLKQFQVCSLSVFGSVARGEAGPDSDID
                     LLVDFEPGARIGFFAFARLQKCLEELLGTKVDLVTPDALHEGMRAEIIEEAVHAI"
     gene            1045..1239
                     /locus_tag="PK442_10260"
     CDS             1045..1239
                     /locus_tag="PK442_10260"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOO88079.1"
                     /translation="MIKNKTSKEMIEIIEADGWTLDRIRGDHHQFVHTTKPGVVTIPH
                     PEKNLGSWLIGKILKQAGLK"
     gene            1271..1681
                     /locus_tag="PK442_10265"
     CDS             1271..1681
                     /locus_tag="PK442_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOO88080.1"
                     /translation="MAAKKDLRIYPALFHFEEEDKLFCVEYPDLPGCVTVGENMEIAL
                     RRAKEALEGFLFSSEQNGYPVAPPTPLEKMSVPAEHILVPVSVRMDILREEMANRSIT
                     KNVTIPAWLNKMAIEHKINFSSTLQEALRERLGV"
     gene            complement(1727..>1920)
                     /locus_tag="PK442_10270"
     CDS             complement(1727..>1920)
                     /locus_tag="PK442_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HOO88081.1"
                     /translation="FRSVKVDVDEAVVLVEAWMVPLREKTVRKNLTIPAKLAYKAERA
                     GINFSGVLTKALEKELQRL"
BASE COUNT          472 a          550 c          471 g          427 t
ORIGIN      
        1 aaactaggaa tccttcaatt aatccggatt atactactaa tccgtcgact tttctataaa
       61 gcctgataat cacttttcca ctaagcgcct gaacgcgcat cgccccgaat cagcagtccg
      121 atccagggcg atgcgcgcag cgtgcgcagc gtagagttga tgtttaattt ctgggcagag
      181 caatagcgat ttgaccttct atgtttcgtc cacttctttt cccgatacaa gcagagcttc
      241 taaaggcact acaatagcag gaagatccat ttgtatcgtt tcccagagaa tctgttcgct
      301 gataccaaag tactcgtgca caatgaaatt cctcatcgcg cgcatttcgg cccaggggat
      361 aggccgatat tttcacagac gaattccggc acacgggccg ccgcctcgcc gatgatcgta
      421 aggcggcgca ctactgcgtc catgttgcgc tggtcccgga cgaagtcgtc gaaggtcatc
      481 ccctctatat aaccaaggat cgtacgaaca gcatcgacaa tatcccgtat acgaagcttc
      541 caacttctag atcgcatgca cggcctcctc gatgatctcc gctcgcattc cttcatgcag
      601 cgcatcgggc gtaacgaggt cgaccttcgt tccgagcagt tcctcaaggc acttctgaag
      661 gcgggcgaag gcgaagaacc caatccgggc gccgggttcg aagtccacca gcaggtcgat
      721 gtcgctgtcc ggcccggcct ctccgcgtgc gaccgaacca aacaccgata acgagcaaac
      781 ctgaaactgc ttcaacagtt ccttgttggt ttcaaggatg ctcaccgtcc gttcaagctg
      841 cgtcatggca attcgcctcc ttatcggaaa gtatatcatc gttactccgc cgccatgcac
      901 agaaaggaac tgatcgtctt cgaggcggca cgggaggaac cacgcctcca tgtggattgc
      961 acttctctat cggctaacat gcaaccccct tttgacacct ataataataa gtgttacaat
     1021 cacctcggat gagagggggc ggaaatgatc aaaaacaaaa cctcgaaaga gatgattgag
     1081 atcatcgaag ctgacggatg gactctggac cgcatcagag gagatcacca tcagtttgtt
     1141 cacacaacga aacccggagt tgtcaccatc ccgcatccgg agaagaacct cggaagctgg
     1201 ctgatcggca aaattctcaa acaagcggga ctgaaataag cctgcttcac ccgcaaagga
     1261 gtgatctgca atggcggcga aaaaggatct gcgcatctat ccggctctct tccactttga
     1321 agaagaggac aagcttttct gcgtcgaata ccccgacctc ccgggatgcg tcacagtcgg
     1381 ggagaacatg gaaatagccc tccgcagggc gaaggaagcg ctcgaggggt ttcttttctc
     1441 ttccgaacag aacggctacc ctgtcgctcc acctactcct cttgagaaga tgagcgtacc
     1501 ggccgaacat attcttgttc ccgtttcggt tcgtatggac atcctgcgcg aagagatggc
     1561 caacagaagc attacgaaga acgtcaccat tcccgcatgg ctgaacaaga tggccataga
     1621 gcataagatc aatttctcca gtacgcttca ggaggcgctt cgcgagcggc tcggggtctg
     1681 aacgcgcatc gcccctgatc ggcagtccaa tccggggcga cgcgcgttat aaacgctgaa
     1741 gctctttttc cagcgctttg gtcagaacgc cggaaaagtt gatccccgcg cgttcggcct
     1801 tatatgccag ctttgccggt atggtcaggt tcttacggac ggttttttcc cgaagcggaa
     1861 ccatccacgc ctctacaagc accacggctt cgtcgacgtc cactttcact gatcggaagt
//
LOCUS       DAOSWW010000546         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50022_length_1920_cov_2.502949, whole genome shotgun sequence.
ACCESSION   DAOSWW010000546 DAOSWW010000000
VERSION     DAOSWW010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50022_length_1920_cov_2.502949"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PK442_10275"
     CDS             <1..931
                     /locus_tag="PK442_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOO88082.1"
                     /translation="LDALCDGTNVYVAGIGEHIEAAGIHSGDSACVFPVYKSPPEILR
                     EMAAAAVSVARAIGVKGFLNIQFAMKDGVLYVLEVNPRASRTVPFLSKASGVDLVKAA
                     VRLWEGKNLEAQGLVRNGYGEGRCLAGWAVKEAVFSFDRFASVDPLLGPEMRSTGEVM
                     GAGESVGEAYAKAQAACGTVLPTKGTVFISVNDPDKPTILPVAKRLREMGFSIAATTG
                     TARYLFENGILPEVVLKVHEGSPNVVDHMRAGRIQMLINTPLGRHSLHGDKEMRIEAV
                     RRKIPYTTTTSAAEAAVEGIAALKEGTLRIRRL"
     gene            complement(982..>1920)
                     /locus_tag="PK442_10280"
     CDS             complement(982..>1920)
                     /locus_tag="PK442_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88083.1"
                     /translation="APSSRVTPFHHLHYGARALGGAGLIIIEATAVLPEGRLSDNCLG
                     LWDDEQIAGMTELTRHIHSLGAKIGVQLNHAGRKCGAAAETIYAPSAVNYSDDGKYKD
                     PVEMNPDEIARVTEAFAAAAGRALQAGFDVVEIHGAHGYLINQFLSPLSNRRRDGYGV
                     NFEGRSRLLQEIARAVRTRWPEEKPLFLRISAEDYVQGGMTTGQMARIVDRVTKEVDV
                     VHVSSGGVTPVAPPVHPGYQIPFAEQIKRECEVPTIAVGLITSLDMIEEALCNQRADL
                     VALGRELLRNPFFPLNEARKRGIELPWPEQYRRAFS"
BASE COUNT          353 a          556 c          632 g          379 t
ORIGIN      
        1 cctcgacgcg ctctgcgacg gaacgaacgt ctacgtcgcc ggaatcggcg aacacatcga
       61 ggccgccggt atccactcgg gcgacagcgc ctgcgtcttc cccgtctata agtcgcctcc
      121 ggaaattctt cgggagatgg ccgccgccgc cgtttccgtc gcgcgcgcga taggggtgaa
      181 agggtttctc aacattcagt tcgccatgaa ggatggcgtg ctctatgtgc tggaggtgaa
      241 tcccagagct tccagaacgg tccccttcct ctcgaaggcg agcggcgtcg acctcgtaaa
      301 ggcggccgtt cgtctctggg aggggaagaa cctcgaagcg caggggcttg tccggaacgg
      361 ttacggagag gggcgttgcc ttgcggggtg ggcggtgaag gaagccgtct tctcgttcga
      421 caggttcgcc tcggtcgacc ctctgctcgg gccggaaatg cgctcgaccg gagaggtcat
      481 gggcgcaggc gagagcgtag gagaggcgta cgccaaggcg caggccgcct gcggtacggt
      541 gctgccgacg aagggaacgg tgttcatctc ggtgaacgat ccggataagc ccacaattct
      601 ccctgtcgcg aagcgacttc gtgaaatggg gttttcgata gcggctacaa caggaacagc
      661 gcgttatctc tttgaaaatg gcattcttcc cgaagtggtg ctgaaggttc atgaaggctc
      721 gcccaacgta gtagatcaca tgcgtgccgg gagaattcag atgctcatca atacccctct
      781 tgggcggcac agcctgcacg gagacaagga aatgcgaatc gaagcagtcc gacggaagat
      841 accgtacact accacgacgt cggcggcgga ggcggccgtg gaggggatcg cggcgctgaa
      901 agagggtacg ctccggatac ggaggctgta gggaaataat gaatcgaggg gcttccgaat
      961 agaggaagcc cctcgcggtt cttagctgaa cgctcttctg tattgctcag gccaggggag
     1021 ctctatgccg cgttttcttg cctcgttcag cgggaagaag gggttacgga gcagctcgcg
     1081 ccccagggcg acgaggtcgg ccctttgatt gcaaagcgcc tcctcaatca tgtcgagact
     1141 ggtgatcagc ccgaccgcga tggtcggaac ctcgcactcc ctcttgatct gctcggcgaa
     1201 gggaatctga tatcccggat gcacaggggg agccaccggg gttacccctc cggagctgac
     1261 gtggacgacg tccacttcct tggtgactct gtcgacgatg cgggccatct gtccagtggt
     1321 catacctccc tggacgtagt cctccgccga aattcggagg aaaagaggtt tctcctccgg
     1381 ccagcgtgtg cgcactgccc tcgcgatctc ctggagcaag cgcgacctgc cttcgaagtt
     1441 tacaccgtag ccgtcccgcc ggcggttgct cagcggcgag aggaactggt ttatcaggta
     1501 gccgtgcgct ccgtggatct ccacgacgtc gaatcccgcc tggagcgctc gccccgccgc
     1561 tgcggcgaag gcttcggtga cgcgcgcgat ctcgtcgggg ttcatctcga cggggtcttt
     1621 gtatttcccg tcgtcgctgt agttgacagc gctgggcgca tagatggttt cagctgccgc
     1681 gccgcacttc cgtccggcat ggttgagctg cacgccgatt tttgccccca ggctgtggat
     1741 gtggcgcgtc agttcggtca tacctgcaat ctgttcatcg tcccaaagtc cgaggcagtt
     1801 gtcggagagc cgtccttcgg ggagaacggc ggtcgcctcg atgatgatca acccggctcc
     1861 cccgagggcg cgcgcgccgt agtggagatg gtgaaagggg gtgacgcggg aagagggcgc
//
LOCUS       DAOSWW010000547         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50072_length_1919_cov_2.287017, whole genome shotgun sequence.
ACCESSION   DAOSWW010000547 DAOSWW010000000
VERSION     DAOSWW010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50072_length_1919_cov_2.287017"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1568
                     /locus_tag="PK442_10285"
     CDS             177..1568
                     /locus_tag="PK442_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO88084.1"
                     /translation="MDPIILEEGLVITHSRSLTSGKISSQLFALALPLLLGNILQQLY
                     NVVAAVIVGQYIGEEAFAAIGVAGTVMNLFIFVLIGGCTGVSIILASLFGSGDFHSLR
                     KESFLAIVFGAGFTLVVSLVSIMLLSPLLESINTPAEIFAYTQQYLLIILAGLPATFF
                     YNLCAAALRAVGNTRYALFFLMIAVAVNVGLTFVLVVLMGWGITGAAWATVFSQGLSA
                     VLCMAYIKKKLPFLVPTREDMRYDGVLLKKTIIFASLSAMHQSSLYIGKILVQGAVNL
                     LGTASIAAYTATARIEGLSLAFGESGSESISVFVAQNTGAGNKKRALKGFGEGLFALV
                     ALGVVATGILYLSAERSIALFIGTHQAAAIAEGVAYMRLVCFFYVLSYTGSSFVGFFR
                     GSGRINIPFIGTTMHLSVRVVLSYWLAPVMGLKAVALATGVGWVSIVLFQLVIFAAIR
                     RRERKLELSGAPL"
BASE COUNT          310 a          563 c          587 g          459 t
ORIGIN      
        1 agagagccgg gggctcgcgc tgaagaagta ggcggatcag aggagaagag caaaaggacg
       61 ccttcgggcg tccttttttg attcctccat attgaggaac tgactgccct gtgcgtccgc
      121 cgcctgtgcg atggactcgt aaaggcggga gcggtttccg cattttctgg cgtataatgg
      181 accccataat tttggaggag gggcttgtca tcacccattc gcgttctctg acatcgggaa
      241 aaataagctc gcagctcttt gcgctggcgc ttcccctgct gcttggcaat atactgcaac
      301 agctgtacaa cgtcgtcgcc gccgtcatcg tcgggcagta cataggagaa gaggcgttcg
      361 cggcgatcgg cgtggcgggg acggtgatga acctgttcat cttcgtgctc atcggcggct
      421 gcaccggcgt ttctattatt cttgcctcgc tcttcgggag cggagatttt cactcgctgc
      481 ggaaagagtc gttcctggcc attgtgttcg gcgcggggtt taccctcgtt gtcagccttg
      541 tctccatcat gctgctttcg ccgctgctgg agagcatcaa cacccccgcc gagatatttg
      601 cgtatacgca gcagtacctg ctcatcattc tggccggtct gccggcgacc tttttttaca
      661 acctctgcgc agccgctctc cgtgcagtcg gcaatacccg ctacgccctc ttcttcctca
      721 tgatcgcggt cgccgtcaac gtggggctga ccttcgtcct ggttgtgctg atgggatggg
      781 gcattaccgg cgcggcctgg gcgaccgtct tttcgcaggg gctgtcggct gttctctgca
      841 tggcgtacat taagaaaaaa ctgccgttcc tcgtgccgac aagggaggac atgcggtacg
      901 acggtgtgct gctgaaaaaa acaatcatct tcgcctccct gtcggcgatg caccagtcga
      961 gcctgtacat aggcaagatt ctggtgcagg gcgcggtcaa cctgctgggg acggcgtcca
     1021 tcgcagccta cacggcgacg gcgcggatag agggattatc ccttgctttc ggagaaagcg
     1081 ggtcggagtc catttcggtg tttgtcgccc agaacacggg ggccggcaac aaaaaacgcg
     1141 cgctcaaggg gttcggcgag gggctgttcg cgctcgtcgc gctcggagtc gtagcgacgg
     1201 gtatcctcta cctctccgcg gagcggtcca tcgcgctctt tatcggcacg caccaggcgg
     1261 ccgcgatcgc ggagggcgtc gcctacatgc ggctggtctg cttcttttac gtcctctcct
     1321 atacgggtag ctcatttgtg ggcttttttc gaggcagcgg acggatcaac atccccttca
     1381 tcgggacgac gatgcatctg agcgtgcgcg tggttctttc ctactggctc gctccggtga
     1441 tggggctgaa ggcggtggcg ctggcaacag gcgtgggctg ggtctccatc gtgctgttcc
     1501 agttggtgat atttgctgct atccgccgca gggaacggaa actcgaactc tccggcgcgc
     1561 cgctgtagtc cgggtttgcg cccaagcaac tctgattttg ttccgcttcc cgctcatata
     1621 taccgggcgc gtcgggagct cttgccgccc ggcgcgcccg gcatgtttct tccggcggtt
     1681 actcgaagac ggtcagttcg ctgtcttcgg ccttcgccgt tgccgacatg attgccagcg
     1741 ctgccgcgcc agccagcagt atcctgccga tattcgctct tttcatgctg gtactccctc
     1801 ttgtcatggt ctgccttgtt atttgcgcag gcccccgtca gtggtgaaat gctccgggca
     1861 aagcacgcca agcgcggcaa gcgcggtgag ggtgctctgt gcgccgccgt tggcataga
//
LOCUS       DAOSWW010000548         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50109_length_1918_cov_2.690821, whole genome shotgun sequence.
ACCESSION   DAOSWW010000548 DAOSWW010000000
VERSION     DAOSWW010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50109_length_1918_cov_2.690821"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PK442_10290"
     CDS             <1..866
                     /locus_tag="PK442_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88085.1"
                     /translation="FYAGINFAVSSLGAIVSVAPLGFALDTFGIPSTFAILAGTSFLM
                     AVFLARRAKSDPMRKAAGDEKKTISSILPGIGRAVLFIFRNRRVRSLMIVWAVSSASL
                     LSFQGLWGVSWFAAAFNASPASARFWSSLISAGMMVGPLMIGGVVLSPEKLPLLVRRV
                     SSVNALVWFLLLGTTVSGLPIWSGGAVALLLGAITGLRGVFALAGVTALAPAGEKGVV
                     FGAMNMIAVLSAIVFQWGTGIVINRFPAGQPGVYTAEGYFAAFLLVSCAMAASLLALR
                     SLGKEPLRQEE"
     gene            complement(960..1073)
                     /locus_tag="PK442_10295"
                     /pseudo
     CDS             complement(960..1073)
                     /locus_tag="PK442_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
     gene            complement(1059..1517)
                     /locus_tag="PK442_10300"
                     /pseudo
     CDS             complement(1059..1517)
                     /locus_tag="PK442_10300"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(1612..>1918)
                     /locus_tag="PK442_10305"
     CDS             complement(1612..>1918)
                     /locus_tag="PK442_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88086.1"
                     /translation="SPFGHSRKQGFLRAAVAPRTHALHVAMQLPRRKKRNYPTREEAK
                     KRLVEKALRFGPDALSAKEKTALLKDTDRLRELHRRVWTLEDEEKAALWGLALPKEE"
BASE COUNT          294 a          604 c          601 g          419 t
ORIGIN      
        1 gcttctatgc ggggatcaac ttcgccgtct ccagcctggg ggccatcgtc tcggtcgcac
       61 ccctcggatt cgcgcttgat acgttcggca tcccctcgac cttcgccatt ctcgcgggga
      121 cgagctttct catggcagtc tttctggcgc gcagggcgaa gtcggacccc atgcgcaagg
      181 cggcgggaga cgagaagaag acgatctcct ccattcttcc ggggatcggg agggcggtgc
      241 tcttcatctt ccggaaccgg cgcgtgcggt cgctgatgat tgtctgggcc gtttcctcgg
      301 cgtcgttgct gagcttccag gggctctggg gcgtctcctg gtttgcggcg gccttcaatg
      361 cctcgccggc gtcggcccgc ttctggagtt cgctgatcag cgcggggatg atggtcggtc
      421 ctcttatgat cggaggagtt gtcctctccc cggaaaagct gcctctgctt gtccgccgtg
      481 taagctcggt gaacgcgctg gtctggtttc ttctgctcgg aacgaccgtc tccggccttc
      541 ccatatggtc gggaggagcc gtggcgctgc tcctcggcgc catcacgggt ttgcggggag
      601 tcttcgctct agcgggggtg accgcacttg ccccggcagg ggagaagggg gttgttttcg
      661 gcgcaatgaa catgatcgcc gttctctcgg cgattgtctt ccagtggggg acggggatcg
      721 tgatcaatcg cttcccggcg ggacagcctg gcgtctatac ggcggagggc tattttgctg
      781 cgttcctgct cgtttcgtgc gcgatggcgg ccagcctgct cgcactgcgt tcgctcggga
      841 aagaaccgct gagacaggaa gaataagccg aaagatccgc cgcaacgcac acaagtaaag
      901 acgcaacaac acacatcgcg atcgctctct tcattgcgcg gaaagtattt ccacgcatat
      961 taaagcgatt ctctgtcctc gtgttccccg atcttccgca ccaccctgca cgggttgccc
     1021 gcagcgaaga cgccttcggg gatgtccttc gtcacgacgc ttccggcgcc gatgacggag
     1081 cgggagccga tcgtcgcacc ggggcagatg atcgcgcccc cgccgaccca gacgtccgat
     1141 ccgataaaga cgggttttcc gaactccttc ccccgccgcg cctgcgcgtc catcggatgg
     1201 gtcgccgtgt agatctgcac tgccgggccg aacatagtga agtcgccgat gcgcacttcg
     1261 cagacgtcga ggacgatgca gctgaagttg aagtacactt tgtcccccag gtgaatgtgc
     1321 acgccgtagt cgcagtggaa gggcggctgc acagtcgcgt gctctccgcc gctcccgagg
     1381 agttcccgca ggataagttg ccgaagcttc tgttccgtcg cgcgcgatgc gttcagatca
     1441 aggcacagat cgcgcgcccg agctctcaag cgcacaagct cgcggtcgga tgggtcgtac
     1501 agttcgcctg cgagcatctt ttcccgttcg gtcattcgtg ttcacactcc tcgttttcgg
     1561 atctcgtcca gcgcacggcg gacgtcgtcg atgcatcttc cgccgtatcc cctattcttc
     1621 cttcggcagc gcgagccccc agagcgcggc tttctcctcg tcctccagag tccagacgcg
     1681 gcggtgcagt tcgcgaagac gatccgtgtc cttcaagagc gccgtcttct ccttggccga
     1741 gagcgcgtcc ggtccgaagc gaagcgcctt ctccacgaga cgtttttttg cctcttcccg
     1801 cgttgggtag ttgcgtttct tcctgcgggg gagctgcatc gcaacgtgga gcgcgtgggt
     1861 gcgcggggcg acggcggcgc gtaaaaagcc ctgcttccgc gagtgtccga acggggag
//
LOCUS       DAOSWW010000549         1917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50138_length_1917_cov_4.174544, whole genome shotgun sequence.
ACCESSION   DAOSWW010000549 DAOSWW010000000
VERSION     DAOSWW010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50138_length_1917_cov_4.174544"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PK442_10310"
     CDS             <1..256
                     /locus_tag="PK442_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88087.1"
                     /translation="LGFVKTENAAVEKYMGNAVGSSSKGREAYVSLFFPEKKRDDVLE
                     LLGVLKNEGLEIALLFELPPSPYTSKDSAKERTLEDFDTL"
     gene            253..1635
                     /locus_tag="PK442_10315"
     CDS             253..1635
                     /locus_tag="PK442_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO88088.1"
                     /translation="MSINAKLGDILRAVTRTGADFADMYFEIAASHSYAFEDGSFEEI
                     SSSSSEGVGARILLGEATSLAYAPGVALETGASCLHEAAEHSGLSLPCRRMPAIRVME
                     REPPFPTPEYAFFYDLDKRIKASSMWIRQVSMNLDTAFKSFSVFNSDGSIAGDRRLYT
                     IFSVEVVVEKNGILQTGYESEAFSVDSGEFFRTVSPFKVAQRALFRALLMLDAPQCPA
                     GAMTVLLSGEAGGTMIHEACGHGFEADIIQKDYSVYRDKIGKQAASSLITLVDDGSIP
                     GYLGSGSFDDEGTPCKRNVLIEKGVVRSYISDIASSRRGALPLTGNGRRSSYRSVPQP
                     RMTNTYIEPGDSSPVEMLHSMNRGLLVKKMGGGEVDPTSGDFVFHVTEGYLVEGGRVL
                     HPVRGAVLTGNGPDVLMNIEAVGNDLHFLPGMCGKSGQSVPVTDGQPSLLVGNLTVGG
                     ANTCDDAIFE"
BASE COUNT          436 a          455 c          537 g          489 t
ORIGIN      
        1 ccttggattc gtcaagacag aaaacgctgc ggttgaaaaa tatatgggga atgctgtcgg
       61 aagctcctcc aaggggagag aggcctatgt ttctctcttt tttccggaga agaaacggga
      121 tgacgtgtta gaattactag gagtccttaa aaatgaaggt ttggagatag ctcttctttt
      181 cgagttgccg ccttctccat atacctcgaa ggattcggcg aaagaacgta cattggagga
      241 tttcgatacg ctatgagcat caacgcaaaa ctaggagata tattgcgggc cgtgacgcgc
      301 accggcgccg attttgccga tatgtatttt gagatagcgg cgtcgcactc gtatgcattt
      361 gaggacggca gctttgaaga gatttcgtct tcttcttccg aaggagttgg cgcacggatt
      421 cttctgggcg aagcgacttc tcttgcgtac gcgccgggag tcgccctgga aacgggcgct
      481 tcctgccttc atgaagcggc ggaacacagc ggtctttccc ttccttgtcg ccggatgcct
      541 gcgattcgcg tcatggagag ggagccgccc tttcctactc cggagtatgc ctttttttat
      601 gatctggata aacgtataaa ggcgagctct atgtggatac ggcaggtatc catgaatctc
      661 gacacggcgt ttaagagttt cagcgttttt aacagcgatg gctctatcgc cggagaccgg
      721 cgtctttata cgattttcag cgtagaggtt gtcgttgaaa aaaacggcat actgcaaacc
      781 gggtatgaat ctgaagcctt ctcagtggac tcaggcgagt tcttcaggac agtttctccg
      841 ttcaaagtgg cgcagagggc gctctttcgt gctctcctta tgctcgacgc ccctcagtgc
      901 cctgcaggcg caatgacggt gctgctctcg ggagaagcag gaggcactat gatccatgaa
      961 gcctgcgggc acgggttcga agcggacatc attcagaagg actattccgt ttaccgggac
     1021 aagataggca aacaggccgc gagctcgttg atcacgcttg tcgacgacgg ttctattccg
     1081 gggtatctcg gaagcggttc ctttgacgat gaaggcactc cctgcaaacg aaacgttctt
     1141 atcgaaaagg gggttgtccg gagctatatc agcgacatcg cctcgtcgag acgaggcgcc
     1201 cttcccctga ctggaaacgg caggcggagt tcctatcgct ccgttccgca accgcggatg
     1261 acaaacacgt atatagaacc gggagattct tcgcctgtgg agatgctgca ttctatgaac
     1321 agaggtcttc tcgtcaagaa aatgggcggc ggagaagtgg atcctacatc aggagatttt
     1381 gtctttcatg ttacagaagg gtatctcgtc gagggaggaa gagttcttca ccctgtacgt
     1441 ggagcggtgc ttactggaaa cggcccggat gtccttatga acatcgaggc agtggggaat
     1501 gatcttcact tcctgccagg gatgtgcgga aagtcagggc agagcgtacc tgttaccgat
     1561 ggacagccat ccctgctcgt tggaaatctc accgtcggag gggcaaacac gtgtgatgac
     1621 gctattttcg agtaaaaaaa agaagacgtt cgtcaaaacg tctgcgagtt ccagaggaag
     1681 aggcaagaga gaaaactcca gcgtactgtc gctttggata gaaacgctca cttttttgat
     1741 cttcgccttt cttctctatg tgctcgcttc gatcttttcc ctcagaacag gcgtttgggg
     1801 ggatgcgatc cgtttttctc tactgcgaaa ctggggcggc gctatcctca ttcctgtttt
     1861 tttcgggggg tatctatgca ttgcctattt tttaaagaca ggcgcaaaag ggtttct
//
LOCUS       DAOSWW010000550         1916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50179_length_1916_cov_3.914562, whole genome shotgun sequence.
ACCESSION   DAOSWW010000550 DAOSWW010000000
VERSION     DAOSWW010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50179_length_1916_cov_3.914562"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..989
                     /locus_tag="PK442_10320"
     CDS             162..989
                     /locus_tag="PK442_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO88089.1"
                     /translation="MAMLALAAMLTLSDVELYYEVHGDGPPLLLIAGMASDSQSWGSS
                     APPLAEHFTVICPDNRGVGRSKPADAPVSIERMADDCAALVRSLGYEKVHVLGHSMGG
                     FVAQTLAIRFPELVDRLVLAGTGSKFSARNAALFSDALETLERGADPVLWFRNFFYWL
                     FTPAFFEQPGMLDAAVNYALNYPWPQETVSFRNQVEAVRAFDVADDMPKITAPTLVLA
                     GAEDLLFTPGECREFAARLPDATLEIIEGAAHSIHMERGNEFVRAIIEFLTDQRGRR"
     gene            986..1564
                     /locus_tag="PK442_10325"
     CDS             986..1564
                     /locus_tag="PK442_10325"
                     /inference="COORDINATES: protein motif:HMM:NF036878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOO88090.1"
                     /translation="MRKVVYIDYENLPGVHIEDVEGTKFLIFIGENQKKIPTDTIVRM
                     QPLGERVEWISISGVGRNALDFHIAYYLAKYHTNNTVTHYILSKDAGYDPLIKHVVKF
                     GQRVKRIITLEEIKEKPVLAPELAQKYAKVLEILKKQDKTRRPKIRKTLASSIETLFQ
                     GQVSKEDTDLLIEHLFRERFIEEKNKRIAYLD"
     gene            complement(1661..>1916)
                     /locus_tag="PK442_10330"
     CDS             complement(1661..>1916)
                     /locus_tag="PK442_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO88091.1"
                     /translation="MALVGSVLGLIAYCYSLRYVTAATASHVCTVEIPSGIGLAALMS
                     GEIPTLQEINGSLLIILAIFLAALPEDFLRRLRKERVVLK"
BASE COUNT          397 a          531 c          588 g          400 t
ORIGIN      
        1 gcgtggaacg ctttctttcg ggaggcgttt ggtctctttc tgaaaagagc cgccgcgtgg
       61 tatagtaatg ccgtccgccc gtgcccttat ggaatgaccg gagaacggcg ggttatggaa
      121 gtctttttcc cgctcttcat ttatctgaaa ggggttttgt gatggcgatg ttggcgctgg
      181 ccgcaatgct cacgttgagc gatgtcgagc tgtactacga ggtgcacggc gacgggccgc
      241 ctctgctgct gatcgccggg atggcgtcgg actcgcagag ctggggctct tcggcgcccc
      301 cgcttgctga gcattttacc gtcatctgcc ccgacaaccg gggagtgggc cgctccaagc
      361 ccgcggacgc cccggtctcg atcgagcgga tggcggacga ctgcgccgcg ctggtccgct
      421 ccctcggata cgaaaaagtg cacgttctcg ggcactcgat ggggggattc gtcgcgcaga
      481 cactggcgat tcgctttccc gaacttgtgg acaggcttgt gctcgccgga acgggttcaa
      541 agttttcggc acgaaatgcg gctcttttct ccgatgcttt ggaaacgctt gaacgcggcg
      601 ccgatcccgt cctctggttc cgcaacttct tctactggtt gttcaccccc gcgtttttcg
      661 agcagcccgg tatgctcgac gccgcggtga actatgcgtt gaactatcct tggcctcagg
      721 agacggtctc ttttcggaac caggtcgagg cggtccgcgc ttttgacgtc gccgacgaca
      781 tgccgaagat tacagcgccg accctagtgc ttgccggcgc ggaggatctt ctgttcacgc
      841 cgggggaatg ccgggagttt gcagcccgcc tgccggatgc gacgctcgaa ataatcgagg
      901 gggcggccca ctccatccac atggagcggg gaaacgagtt tgtacgcgca atcatcgaat
      961 tcttaaccga ccaaaggggg cgccgatgag aaaggttgtc tacattgatt acgagaatct
     1021 tccgggagtc catatcgaag atgtggaggg gacgaagttc ctcattttca tcggggagaa
     1081 tcagaagaag attcccaccg ataccatagt ccggatgcag ccgctcggcg aacgggtgga
     1141 gtggatttcc atcagcggcg tggggcggaa cgcgctggac tttcatatcg cgtactacct
     1201 ggcgaagtac cacacgaaca atactgtgac ccactacatt ctctcgaagg acgcggggta
     1261 cgacccgctg atcaagcacg tcgtcaagtt cggccagagg gtcaagcgca tcatcacgct
     1321 cgaggagatc aaggagaagc ccgtcctcgc cccggagctg gcgcagaagt acgccaaagt
     1381 gttggaaata ctgaagaaac aggacaagac gcgccgtccg aagatacgca agacgctggc
     1441 gtcgagcatc gaaacgctct ttcaggggca ggtttcgaag gaggataccg acctccttat
     1501 cgagcacttg ttccgcgagc ggttcatcga ggagaagaac aagcgcatcg cctatctgga
     1561 ctgagagttt tttcaaagcg caaaataaaa ggaaggaacc gcccgaagcc ggttccttcc
     1621 ttttattttg ggttactttt tagaaagaac ggattcccgc ctacttcagg acgacacgtt
     1681 ccttccgcag tctgcgcagg aagtcctcgg gaagggcggc gaggaagatc gcaaggatga
     1741 tcagcagact gccgttgatc tcctggagcg ttgggatttc accgctcatg agagcggcaa
     1801 ggccgatccc cgagggaatt tccacggtgc agacgtggct cgccgtcgct gcagtgacgt
     1861 agcgcaggga atagcagtac gcgatgagcc cgagcacgct gccgacgagc gccatg
//
LOCUS       DAOSWW010000551         1915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50224_length_1915_cov_3.623656, whole genome shotgun sequence.
ACCESSION   DAOSWW010000551 DAOSWW010000000
VERSION     DAOSWW010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50224_length_1915_cov_3.623656"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK442_10335"
     CDS             <1..190
                     /locus_tag="PK442_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88092.1"
                     /translation="ALLRVMAYGAGHSTVIIAAGSATDVFSRFISKGGQGSVWLGRIS
                     GLLLLAAAAYLVYNAPTV"
     gene            224..763
                     /locus_tag="PK442_10340"
     CDS             224..763
                     /locus_tag="PK442_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO88093.1"
                     /translation="MDFVDLVRKRYSLRKYSPFPIAVPVIEKCLEAARLAPSACNSQP
                     WSFLVAATPEKVKPLAEAAFSGVYSMNSFAAGAPVLIVAITERSRYSAALGGMFRGVQ
                     FSLMDVAIACEHISLQAAELGLGTCMFGWLNEKAVKKALDLPSSAKIDLMLSMGYPED
                     EEFLPPKRRKSLEEIRRFL"
     gene            858..1412
                     /locus_tag="PK442_10345"
     CDS             858..1412
                     /locus_tag="PK442_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOO88094.1"
                     /translation="MIALYVILGIAALVLLWGIGIYNGLIKKVNLKNEAWSGIDVQLK
                     RRFDLIPNLIETVKGYAGHEKEVFLKVTEARSAIGQSKSVAERAESENMLSGALKSLF
                     AVAEGYPELKANTNFLQLQEQLSSLENDIQMSRRYYNGAARDYNIAIATFPAVIVANM
                     FGHSKADYFEAVEEERSAPKVSFQ"
     gene            1424..>1915
                     /locus_tag="PK442_10350"
     CDS             1424..>1915
                     /locus_tag="PK442_10350"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOO88095.1"
                     /translation="MIMKRLAGVLALLTFLLLPLSAGAQEKIHLFSSRATLLADSSLE
                     VREDITVNVEGRQIRRGIYRDFPTKYTDSSGRTVRVGFDVLETLLDGREVPFKTESIA
                     NGVRVYIGDPDEFAPLGKRTYTLSYVTTHQIGFFEGHDELYWNVTGNGWDFVIDEARF
                     TLSI"
BASE COUNT          424 a          551 c          545 g          395 t
ORIGIN      
        1 cgcgctgctc cgtgtgatgg cctacggcgc gggacacagt acggtgatca tcgcggccgg
       61 aagcgccaca gacgtcttct ccagatttat ctcgaagggc ggacagggaa gcgtctggct
      121 gggcagaatc agcgggcttc tcctcctggc ggctgctgcg tacctggtgt acaatgctcc
      181 tacggtataa ttgcttattc cgaatcccaa ggagggatcg cctgtggact tcgtcgatct
      241 tgtccggaaa cgctacagcc tgagaaagta cagcccgttc cccatcgccg taccggtgat
      301 cgaaaagtgt ctcgaagcag cccgcctcgc tccgtcggcg tgcaactcac agccgtggag
      361 ttttcttgtc gccgcaacgc ccgaaaaggt gaaaccgctt gccgaggcgg cgttcagcgg
      421 cgtctattct atgaactcgt tcgcagccgg cgcgccggtg ctcatcgtcg cgatcaccga
      481 acgctctcgg tacagcgcgg ctctcggcgg catgttcaga ggagtgcagt tcagcttgat
      541 ggatgtggcg atcgcatgcg agcacatctc cttgcaggcg gcggaactcg ggctcgggac
      601 atgcatgttc ggctggctga acgagaaagc cgtcaagaag gcgctcgatc ttccgtcctc
      661 cgccaagatc gacctgatgc tgtccatggg gtaccccgag gacgaggagt tccttccccc
      721 gaaacgacga aaaagtctcg aagagatcag gcgttttttg tagcgcagcc ggtacgtata
      781 ttttcctgtg cgcttccttc cgggaatggt agaatgatgg caagaaatcc attgattacg
      841 ccaaggaggg aaagaaaatg atagcactgt atgtgatact cggtattgcg gcgctcgttc
      901 tgctttgggg aatcggcatt tacaacggtc tcatcaaaaa ggtgaacctg aaaaatgaag
      961 cgtggagcgg catcgacgtg cagctgaaaa ggcgcttcga cctcatcccc aacctcattg
     1021 aaacggtcaa ggggtatgcg gggcacgaga aagaggtttt tctgaaggtt acggaagccc
     1081 gctccgctat aggccagtcg aagtcggtcg ccgaacgggc ggaatccgag aacatgctct
     1141 ccggcgcgct gaaatccctc ttcgcggtgg ctgaaggcta ccccgaactg aaggccaaca
     1201 cgaacttcct ccagcttcag gaacagctct cctccctgga gaacgatata caaatgtccc
     1261 gccgttacta caacggcgcg gccagagatt acaatatcgc catcgcgacc ttccccgcag
     1321 tcatcgtggc caacatgttc gggcacagca aggcggacta cttcgaggcg gtagaggaag
     1381 aacgttcggc gccaaaagtg tcgttccaat agacggaagg attatgatca tgaaacgcct
     1441 cgcgggagta ctcgcgctcc ttacatttct tctcctgccg ctctcagccg gggcgcagga
     1501 gaagatccat cttttctcct caagggcaac gctcctcgcg gactcctcgc tcgaagtccg
     1561 cgaggacatc accgtcaacg tcgagggcag gcagatacgc cggggcattt accgcgattt
     1621 ccctacaaaa tatacagatt cttcagggcg tacggtgcgg gtaggtttcg atgttctgga
     1681 aacgctcctc gacggccggg aagtcccgtt caagacagaa tcgatagcca acggagtccg
     1741 cgtgtatatc ggcgaccccg acgaattcgc tcctctcggc aaacggacct acacgctgtc
     1801 ttacgtgaca acgcaccaga tcggtttttt cgagggacac gacgagctct actggaacgt
     1861 cacaggcaac ggttgggact tcgtcatcga cgaagcgcgc ttcaccctct ccatc
//
LOCUS       DAOSWW010000552         1913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50322_length_1913_cov_2.921421, whole genome shotgun sequence.
ACCESSION   DAOSWW010000552 DAOSWW010000000
VERSION     DAOSWW010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50322_length_1913_cov_2.921421"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PK442_10355"
     CDS             <1..973
                     /locus_tag="PK442_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOO88096.1"
                     /translation="SDAASSAVCALAERDKRVVCLTAAMKCGSKLDTFAERFPDRFFD
                     VGIAEGHMLTMAAGMAAGGLRPVVFIYSTFLQRAMDQLVHDIAMQNLPVTIAVDRSGL
                     IGDDGETHQGLLDISWSRSVPHLVVAAPRDEIGLSALFDLGLRRNGPMMIRFPRGEAI
                     PSLHRIRGEVFLDDLPVPEILEKGTECALLGYGKTVELMLDAREIIVRDGGVPPSVVD
                     IKCVKPMCVPVMEGLLKSHELVIVAEDGYKECGVGEAIASLAEKLRPSSRTAMVVPLG
                     IPDLFVPQGTVSEQAEYCGLTPRKVVTMYEEYQKRTHRQGSGIQGVR"
     gene            915..1742
                     /locus_tag="PK442_10360"
     CDS             915..1742
                     /locus_tag="PK442_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOO88097.1"
                     /translation="MKSIKKERIDKVLVSRGFVETRTKAQSLLMAGKVTVDGNRVDKP
                     GALVFPDAAVEVIGTGNEWVSRGALKLLKALDVFPFDPKDRICVDIGASTGGFTQVLL
                     SRGALRVYSVDVGYGQLAWSLRSDERVVVMERTNARNLLPDNFPDIPDRAVADASFIS
                     LKLLLPAMDTILSPQGEAVVLVKPQFEVGKGKVGKGGVVRSSEDHIVVLEEILNFCLR
                     ETTFTPCGITFSPITGPMGNIEYLLYLSKSDSEPSIPHPVSIVEEAHSFFTKGRAAS"
BASE COUNT          416 a          465 c          573 g          459 t
ORIGIN      
        1 gagcgacgcc gcttcaagcg ctgtgtgcgc tcttgcggag cgcgacaagc gggtggtctg
       61 ccttactgct gcgatgaagt gcggcagtaa gcttgacacg ttcgcggagc gtttccctga
      121 tcgtttcttt gatgtaggca tagccgaggg gcacatgctc actatggcgg ctggtatggc
      181 ggctggagga ttacggccgg tagtgtttat ctactcgaca ttcctccagc gagcaatgga
      241 tcagctcgtc catgatatcg ctatgcagaa tctcccggtt accatcgcag ttgaccgttc
      301 ggggctcatc ggcgacgacg gagagacaca tcaaggattg ctcgacattt catggtcgcg
      361 ttccgtacct caccttgtcg tggcggctcc aagggacgag atagggcttt cagcgctctt
      421 cgatctgggg cttcgtcgaa acgggccgat gatgatccgc ttccccaggg gtgaggcgat
      481 tccctccctt cacaggattc ggggcgaagt ttttctggac gaccttcctg ttccggaaat
      541 cctggaaaag ggaactgagt gcgcgctcct ggggtacgga aagaccgtcg aactgatgct
      601 ggatgcgcgc gaaataattg ttcgcgacgg cggcgttccc ccttctgttg tggacatcaa
      661 atgtgtcaaa cccatgtgcg ttccggtaat ggaggggctc ctgaaaagcc atgaacttgt
      721 catcgttgcc gaagacggtt acaaagagtg cggagtaggc gaggcgatcg cgtcccttgc
      781 cgaaaaactc aggccgtcgt cccgaacagc catggtggtt ccgttgggca tccccgatct
      841 tttcgttcct caggggacgg tttctgaaca ggccgagtac tgcggtctta ccccgcgtaa
      901 agtggtgaca atgtatgaag agtatcaaaa aagaacgcat cgacaaggtt ctggtatcca
      961 gggggttcgt tgaaaccaga acaaaagcgc aaagccttct gatggccggg aaagttacag
     1021 tcgatgggaa tcgggtggac aagccgggcg cgcttgtctt tcctgacgcg gctgtggaag
     1081 tgatcggaac cggaaacgaa tgggtcagcc gcggcgcact caaactgctg aaagctctcg
     1141 atgtctttcc ttttgacccc aaagacagaa tttgcgtcga tatcggcgcc tcaacgggag
     1201 ggttcactca agttctcctt tcaaggggcg ctcttcgggt atattccgtc gatgtcgggt
     1261 atgggcagtt ggcttggtcg cttcggagcg atgagagagt cgttgtaatg gaacgtacga
     1321 atgccaggaa tttgcttccg gacaattttc ctgatatacc cgacagggcg gttgctgatg
     1381 cgtctttcat ttctctgaag ttattgctgc cggctatgga tacaattctt tccccccaag
     1441 gagaagcggt tgtccttgtt aagccgcagt ttgaagtcgg taaagggaaa gttgggaagg
     1501 gaggcgtggt ccgttcgagt gaggatcaca tcgtcgtttt ggaggagatt ctgaatttct
     1561 gtttacggga aacgacgttc acgccatgcg gtataacatt ttcacccatt accggtccta
     1621 tggggaatat cgagtatctc ctctatctct cgaagtcaga ctcggagcct tcgattccac
     1681 acccagtctc gatagttgaa gaagcgcata gttttttcac gaaaggacgg gcggcatcat
     1741 gaacactccc aatattggca tgcttgtcaa tacgcgaaaa cccggggcga taaaaatggc
     1801 aaggatgctt ttgcagtggg ggatggacaa aggaatcgcg ttcctcttcc ctccgcatga
     1861 ggcgtcggtg ctgggagtcc agggggtgag cgacgaagag tggaaagaga ctg
//
LOCUS       DAOSWW010000553         1912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50375_length_1912_cov_2.453419, whole genome shotgun sequence.
ACCESSION   DAOSWW010000553 DAOSWW010000000
VERSION     DAOSWW010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50375_length_1912_cov_2.453419"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..812)
                     /locus_tag="PK442_10365"
     CDS             complement(174..812)
                     /locus_tag="PK442_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOO88098.1"
                     /translation="MVIKNKLGIDDQTELARAEEKTSKEKALALFELKLFDTLEAGTF
                     KGLSDIHRYLFEDIYDFAGKLRTENIAKGNFRFAPVMYLEMSLQNIEKMPQSTFDEIV
                     EKYVEMNAAHPFREGNGRSARIWLDVILRKELKLVIDWSMVDKNDYLLAMERSPIKDV
                     EIKALLFAALTDKIDDRDVYMKGIDASYHYEGYNVFRTKDLNSRHCDYNKRS"
     gene            1223..>1912
                     /locus_tag="PK442_10370"
     CDS             1223..>1912
                     /locus_tag="PK442_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88099.1"
                     /translation="MRQTRCAIFLFCILFSVLSTPYASSALVHDFDISYYRYLNTAIV
                     KNPDASKIEEIIRYWADAIYEASNGLEHLGTVRIFQGPSSFLSAYRLSDIVWEKAGHP
                     KAAYGAYGSYDAQKPLGFRMAMFDGFKEYASGTAICDFLAPEHRMYAGYALASLSSYY
                     IHDLREEYAIERGGKKYNALAYPSILNDVSLAAYAGDLRALNHSSPRLYTDAPSTVQK
                     AKWGISAWEYLT"
BASE COUNT          453 a          528 c          425 g          506 t
ORIGIN      
        1 cgctctgaga gagccgcata gcgcgcacct gaagagccac ccgccgcgcc ggaacggagg
       61 ccgactttca tcggaaaagt gggcccgaaa agatcggacc ggtggctctc ggagatcgta
      121 acattcacct tctctctact ctgcatgtag aaaggatcgg gactctttga aaatcaactt
      181 cgcttgttgt aatcacaatg ccgggaattc aaatccttcg tcctgaacac gttgtatcct
      241 tcataatgat agctcgcgtc gatgcctttc atgtagacgt ctcggtcgtc gattttatcc
      301 gtcaaagccg caaaaagaag cgccttgatt tcgacatcct tgatagggct tctttccatg
      361 gcgagaaggt aatcgttttt atccaccatg ctccaatcga tcaccagttt caactctttt
      421 ctcaagatga cgtcaagcca tatacgagcg cttctgccgt tcccttctct gaacggatga
      481 gcggcattca tctccacgta cttttcaaca atctcatcga aagtggattg gggcattttt
      541 tcgatgtttt gaagagacat ttcgaggtac attacggggg cgaagcgaaa attacccttg
      601 gcgatgttct cggttcgcag ttttcccgca aagtcgtaga tgtcttcgaa caaatatcga
      661 tgaatatctg acaatccctt gaaggttccc gcttcgagag tatcgaaaag ttttaattca
      721 aacaatgcca gtgctttttc cttgcttgtt ttttcttcag cacgagcaag ttcggtctga
      781 tcatcaatac ccaatttatt ttttatgacc acgaattgat tcctcctgtc aaacgagctt
      841 tgaaaaaaac tctaagtaaa aatatatcac gaacaacacc ttcaatccgc tgtccagtcc
      901 ctacatcgag ccactctctg ccccggcgct cagagtcctt cgtgccacgc tcgtttctcg
      961 cgcctctcaa cgcctccatc gctttgtcga gacccatttt gatgaacagt tcgattatgg
     1021 aaaagaaatc tccaagagag ggaagctttg tgcatctcga tatcgatttt tcccgtgttc
     1081 tttgccataa tagatgcacc cctttggtga aggcctgtgt ctacactacc ttatcactgt
     1141 tctacgaaca gtccagaggg ttttttctgc ctgaggatta ggggctactg aaaaatccat
     1201 aaaaccaaag gaggagttct ctttgcgaca aacacgttgt gctattttct tattctgtat
     1261 tcttttctcc gttctgtcga ccccatacgc atcgtccgcg cttgttcacg acttcgacat
     1321 cagctactac cgctacctga acacagccat agtcaagaat cccgacgcct ccaaaatcga
     1381 agagatcatc cgctactggg cggacgcgat ctacgaggcc tccaacgggc tcgagcatct
     1441 ggggaccgta cgcatttttc aggggccttc aagctttctc tcggcctacc gtctctccga
     1501 catcgtctgg gagaaggccg gccatcccaa agcggcctac ggcgcgtacg gctcctacga
     1561 cgcgcagaaa cccctcggat tccgcatggc catgttcgac ggcttcaaag agtatgcttc
     1621 gggaacggcc atctgcgatt ttcttgctcc cgaacaccgg atgtacgcgg gatacgcgct
     1681 tgcaagcctc tcaagttatt acatccatga ccttagggaa gaatacgcga tcgaacgggg
     1741 cgggaagaag tacaacgccc tcgcctatcc cagcatactc aacgacgtct cgcttgccgc
     1801 gtacgcggga gacttgcgtg cattgaacca ttcctcaccg cgcctttata ctgatgctcc
     1861 ttcgaccgtc caaaaggcga agtggggaat ttctgcgtgg gagtatctga cg
//
LOCUS       DAOSWW010000554         1910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50456_length_1910_cov_4.771429, whole genome shotgun sequence.
ACCESSION   DAOSWW010000554 DAOSWW010000000
VERSION     DAOSWW010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50456_length_1910_cov_4.771429"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PK442_10375"
     CDS             complement(<1..1121)
                     /locus_tag="PK442_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO88100.1"
                     /translation="MKTTMDSSMKQRLVQLRREFHAHAETGWTEFWTTAKIAGILKNL
                     GYQVSVGKEILDLESVMGRPSEDEMAKYIERAVHQGADPEWIKRMDGYTGAVAVLDTG
                     KKGPTLAFRFDIDAVDVAEAAEENHRPFREGFASMNENAMHSCGHDAHTVIGLGFAEL
                     LMRRKEELKGVFKLIFQPAEEGVRGGKAVAMKGILDDVDYFLGAHIGMGIPTGNVAPG
                     CGDFLCTTKFDAVYKGKAAHAGGAPNEGKNALLAAASATLALAGISPHKDGATRLNVG
                     VLHAGTGRNVVPSKAVMKVETRGLTTALNEYVYGRAMEIVEGAAVMYGVELSVAKMGE
                     AVDAFSDKELVDLVAEKALSVPGIEKLAPSVSLGGSEDVS"
     gene            complement(1118..>1910)
                     /locus_tag="PK442_10380"
     CDS             complement(1118..>1910)
                     /locus_tag="PK442_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO88101.1"
                     /translation="EANPVGGAPAMIQQGALKAPEVDAIFGMHVWPSLVTGTIGTRSG
                     ALMGASDRLFLTVRGKGAHGSEPENGVDAIAIAAQILTALQTIVSRSVSPLDAAVVSV
                     GTIKGGNRYNVIADEVLLEGTVRTINPETQEKMPGRIEALVRGIARGMGGDCDMEYVK
                     GYPPLMNDPELAKTAFSVLKKVVGEEKIVYVEKPALGGEDFAFFSKEIPALFLWLGCR
                     PEGVAREDMPPLHNNMFLPDEKALSLGVETLVSCALEYLEGRKGA"
BASE COUNT          427 a          599 c          435 g          449 t
ORIGIN      
        1 gaaacatcct cgctgccgcc aagactgacg gagggggcaa gtttctcaat acccggaacg
       61 gaaagcgcct tttccgccac aagatcaacg agctctttgt cgctgaatgc gtcgacagcc
      121 tctcccatct tggcaaccga cagttccaca ccgtacatga ctgccgcacc ctcgactatt
      181 tccatcgctc gaccgtacac atactcgttc aacgcggtag tcagaccgcg agtttccact
      241 ttcattaccg ccttcgaagg gacgacattg cggcctgtcc ccgcatgcag aacaccgacg
      301 ttgaggcggg ttgctccatc tttgtgcggc gaaatcccgg caagagccaa agttgcggac
      361 gccgccgcca gaagagcgtt ttttccctcg ttcggcgcgc ctccggcatg cgcggctttc
      421 cccttgtaga cggcgtcgaa cttcgttgtg caaagaaaat cgccgcatcc aggagcaaca
      481 tttcccgttg gaatgcccat accgatatgc gcgccgagga agtagtcaac atcatccaga
      541 atgcccttca tcgctaccgc ttttcctccg cggacgccct cttcagcagg ctggaatatt
      601 aatttaaata ctcccttgag ttcttcttta cgcctcatga gaagttctgc aaaaccaaga
      661 ccaatcaccg tatgcgcatc atgcccgcac gaatgcatag cgttctcgtt catggatgca
      721 aacccctcgc ggaagggacg atgattctct tcggcagcct ccgcaacatc caccgcatcg
      781 atatcgaaac gaaaagcgag cgtcgggcct tttttccccg tatctagcac ggcgactgct
      841 cctgtatatc cgtccatccg tttaatccac tccgggtcgg ctccttggtg tactgcacgc
      901 tcaatgtatt tcgccatctc atcttccgac gggcgtccca taacggattc caaatcgaga
      961 atttccttac caacggaaac ctgatacccg aggttcttta aaattcctgc aattttcgcc
     1021 gtcgtccaga actccgtcca gccagtctcg gcatgtgcat gaaactctct tctcaattga
     1081 acgagtcgtt gcttcatcga actgtccatc gtcgtcttca cgctccttta cgtccttcca
     1141 gatattcaag agcgcaggaa acaagcgttt ccaccccaag agaaagcgct ttttcatccg
     1201 gaagaaacat gttgttatga agaggaggca tatcctctct ggctactccc tcaggcctgc
     1261 atccaagcca aagaaaaaga gcggggatct ctttgctgaa gaaagcgaag tcttctcctc
     1321 caagagccgg cttttctacg tacacaatct tttcttcccc gacaaccttc ttcaaaacag
     1381 aaaaagcggt ctttgcaagc tcgggatcat tcatgagggg aggatatccc ttgacgtact
     1441 ccatgtcgca atccccgccc atacctcttg cgataccacg gacgagcgct tctatacgac
     1501 cgggcatctt ctcttgcgtt tcagggttga tcgtccgcac tgttccctca aggagcactt
     1561 catcggctat aacattgtac ctgtttccgc ctttgattgt acccacgctt acgactgccg
     1621 catcaagagg actcacgctg cgcgagacga tcgtctgcag cgcggtgagt atctgcgccg
     1681 ctatggcaat ggcgtcaact ccgttttcag gttccgagcc atgggcgccc ttccctcgca
     1741 cagtcaaaaa gaggcgatcg gaagctccca taagagcccc tgaacgcgtc ccgatcgtgc
     1801 ctgtaacaag agagggccac acgtgcatgc cgaaaatagc gtcgacctca ggcgctttga
     1861 gggctccttg ctggatcatc gcaggcgccc ctccgaccgg attcgcctcc
//
LOCUS       DAOSWW010000555         1910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50462_length_1910_cov_3.345553, whole genome shotgun sequence.
ACCESSION   DAOSWW010000555 DAOSWW010000000
VERSION     DAOSWW010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50462_length_1910_cov_3.345553"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="PK442_10385"
     CDS             <1..1233
                     /locus_tag="PK442_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88102.1"
                     /translation="REISAETISCRMTETRTYSGGIWCPTVETGFWVAQRDGVVFITG
                     NSLSAANVGGLGLITGHALGNYMSPEQARFVARNWVTMYLVALLIVPNILQAAIYGLG
                     HALPGDDDDEQQEKEDRMFATFNEAGKGQFFPHIDITPLMRKVPGYKGDPTGRRRTYV
                     RFGKQSYEVLNGWLKDPMQTAGRKSSMLVKAVWEQLTGYAPGSPDYELPFRGAGPIMG
                     LIDSKIGFRGSRIGTLLSKALPMTISSALSNPDAFITSIVVPASKGMSQTRAIQGMMG
                     LLRAYAEGGNEGIFMRHPEQRANLNSLAARYVDALERNGYDSKQVITSAKGDIMRTLY
                     ADFYEGFVKGDEARMHDAAKRIYRVQGTTDGLLRSVDSRQEKTGRPPLTETERTRVLE
                     AFEGAVPDFVEKTFEGEE"
     gene            1241..>1910
                     /locus_tag="PK442_10390"
     CDS             1241..>1910
                     /locus_tag="PK442_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88103.1"
                     /translation="MNFFSNIISGVTNFFTPQAPSAPPVSSFSNPVVTESVTRVRDFV
                     APMLEKPTFSFAYQPSNVGANRVYSAATAEAPAAPAPRYDEYVAPSIGPAKLPADLKA
                     PLTGSVVNMHVQQRAYDGLGSKMSLLEKAREAGYAPNFDVEQLLYDPGAGARYFREAG
                     KVPLLFDYVPISEPNTYLEKKAAQMGGVTRGLRDAAAVGYLAPGLPAASSAVGKIGNL
                     TLSGV"
BASE COUNT          399 a          565 c          607 g          339 t
ORIGIN      
        1 agggaaatct ccgcggagac catatcctgc cgcatgacgg aaacgcgtac ctactccggc
       61 gggatctggt gccccacggt agagacgggg ttctgggttg ctcagcggga cggtgtcgta
      121 ttcataaccg gaaactccct ctcggccgca aacgtcggcg gcctagggtt gataactggg
      181 cacgcgttgg gcaactatat gtccccggag caggcacgct tcgtcgctag aaactgggta
      241 accatgtatc ttgtggctct gctgatcgtc cccaatatcc tgcaagcggc catctacggg
      301 ctcgggcacg cgctgcccgg agacgacgac gatgagcagc aggagaagga agaccggatg
      361 ttcgccacgt tcaacgaagc cggcaagggg cagtttttcc cgcacatcga catcacgccg
      421 ctgatgcgca aggtcccggg gtataagggg gacccgactg gccgacgccg cacttacgtt
      481 cggttcggaa aacagtcgta tgaagtattg aacggatggc tgaaagaccc gatgcagacg
      541 gcggggcgca agtcgtcgat gctcgtgaag gccgtatggg aacagctgac gggctatgcc
      601 ccagggtctc ccgactacga gcttcctttc cgcggggccg ggccgatcat gggccttata
      661 gacagcaaga tcgggttccg cgggtcgcgt atcggcaccc tgctgtccaa ggcgctgccg
      721 atgacgatct cttcggcgct cagcaacccg gacgctttca tcacctcgat tgtcgtgccg
      781 gcctcaaaag gcatgagcca aacccgggcg attcagggaa tgatggggct gctccgtgca
      841 tacgcagagg gcgggaacga ggggatcttc atgcgacacc ccgaacagcg cgcaaactta
      901 aactcgctgg ctgcccgcta cgtggacgcc ctggagcgga acggttacga ctcaaagcaa
      961 gtcataacgt cagcgaaggg agacatcatg cgcacgctgt acgcagactt ctacgagggg
     1021 ttcgtaaagg gcgacgaagc ccgaatgcac gacgcagcga agcgaatata ccgcgtgcag
     1081 ggcacgacgg atggacttct gcggtccgtg gacagccggc aggagaagac cggacgtcct
     1141 ccgctgaccg agacagagag gacgagagtc ctagaggcgt tcgaaggagc ggttccggac
     1201 ttcgtggaga agacctttga gggagaggag taggccgccc atgaacttct tctcaaacat
     1261 catctcggga gtgacaaatt tcttcacgcc ccaggcccca agcgcccctc ctgtctcgag
     1321 cttctccaac cccgtggtga ccgaatccgt cacgcgcgtt cgtgacttcg tcgccccgat
     1381 gctggagaag ccgacgttct cttttgcgta tcagccgagc aacgttgggg cgaatcgggt
     1441 ctactccgca gcgaccgcgg aagccccggc ggcccccgcg ccgcgatacg acgagtacgt
     1501 ggccccgtcg atagggcccg ccaagctgcc ggcggacctg aaggcccccc ttaccgggtc
     1561 cgtcgtgaac atgcacgtgc agcaacgcgc ctatgacggt ttgggcagta aaatgtcgct
     1621 gctggagaaa gcgcgcgagg ccggctacgc gccaaatttt gatgtagagc agcttctgta
     1681 tgatcccggg gcgggcgccc gctactttcg agaagcaggg aaagtcccgt tgttatttga
     1741 ttatgttccg atttcggagc caaataccta tttggagaag aaagccgccc aaatgggggg
     1801 cgtaacccga ggcctgcggg acgccgccgc cgtcgggtac ctggcccccg ggctgccggc
     1861 agcttccagc gcagtgggga agatcggcaa tctgaccctt tcgggagttg
//
LOCUS       DAOSWW010000556         1909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50506_length_1909_cov_3.756203, whole genome shotgun sequence.
ACCESSION   DAOSWW010000556 DAOSWW010000000
VERSION     DAOSWW010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50506_length_1909_cov_3.756203"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..830
                     /locus_tag="PK442_10395"
     CDS             138..830
                     /locus_tag="PK442_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88104.1"
                     /translation="MTYINVLVVEDDPMVAEIHQNFINSVDNFRVAGIVNNGLKALEF
                     IERRPVRLVILDIFLPGLDGIATLEKIRERGENIDVIVVSASRDVSTVAKAIQAGAFD
                     YIVKPFVFERIRSSLLSFREMMGHLKGTTQEIDQQTIDTLFQVRNRKHYDSKLPKGLN
                     PHVLERLETMLSGTNVSLSSAEAAEALEISRITARRYLEFLVSTGKAVMEQEYHEVGR
                     PVNKYTLMKQTP"
     gene            1166..>1909
                     /locus_tag="PK442_10400"
     CDS             1166..>1909
                     /locus_tag="PK442_10400"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOO88105.1"
                     /translation="MKALRFSRVFFACVLAVFVSTSAVACTPMGVGPKASVDGSVMVS
                     HTCDGWYDHRIQIVPGGSHQPGEMVDIFNIMCYDTRPDRPVKKVGEIPQAEKTYTYFH
                     IGYPFMNDQQVMMGEFTWSGRDELGTPEGLFYIENLEALGLARAATAREAIKVMGSLA
                     EKYGYADGGETLVIADTKELWVFEICGPGLWNKESGRPGAHWVAQRVPDDEVFVGANR
                     ARIGVIDFADTENYIFSTDITKLPEQMGWW"
BASE COUNT          472 a          482 c          488 g          467 t
ORIGIN      
        1 ggcctcttca atatcaagac catagttgat tctctcgccg gcaacatatc aatggatttt
       61 gaaaccgggc gctttactga gtttacggtg acgttgcctg taaccgcggg cgctctccgg
      121 ccggaggtag ccatatagtg acatacatca atgttctcgt agtggaagac gatccgatgg
      181 tcgctgaaat ccatcagaat ttcattaact ccgtcgataa tttccgtgtt gccggaatag
      241 tcaataacgg tctgaaggcc ctggaattca tagaacgccg ccctgtcaga ctggtcattc
      301 tggatatttt cctccccggt ttggacggaa tagccactct cgaaaaaata cgggagagag
      361 gtgagaatat tgacgtgatc gtcgtttccg catcaaggga tgtttctaca gttgcgaagg
      421 caattcaggc gggggcattc gactatattg taaaaccctt cgttttcgag cgaatccgct
      481 catcccttct ctctttccgc gagatgatgg gacatctcaa gggaacaact caagagattg
      541 accagcagac gatcgatacc ctttttcagg tgcgaaacag gaagcactac gacagcaaac
      601 tccctaaagg cctcaacccg cacgtactag agcgtctgga aacaatgctc tccgggacga
      661 atgtgtcgct ttcttcggca gaagcggcag aagcgctgga aatatcacgg atcaccgcta
      721 gacgctacct ggagtttttg gtatcaacag gaaaagctgt catggaacag gagtatcacg
      781 aggtgggacg tcctgtgaat aaatatacgc tcatgaagca aaccccatag cgagagtctc
      841 gtatacggta ttcttacgct ttttcataag aatttttgaa cacgctcttc gttttgtaca
      901 tttttgttca cggcttggca caaaccagcg aaaagatttc ttcagtttcc atcgccagtt
      961 ccgtttcccc ttcccccttt ttttccaggg agttttgaac aaaaagaaac aaaaatgcgc
     1021 acatcattca aaacgactgg cgcaatttac tgcgagcacg tagagtaaaa gagtctctac
     1081 gacgctccgt ttttttcttc tgcggggggg cggatttttc tgtgcgaggc aatgccaatc
     1141 acatattttt ttaggaggta aaaggatgaa agctctgcgt ttttcacgtg tctttttcgc
     1201 atgcgttctt gcggtcttcg tatcgacgtc agccgtggcg tgtactccca tgggagttgg
     1261 tccaaaggcg agcgtcgacg gatcggtcat ggtaagccac acgtgcgacg gatggtatga
     1321 ccaccgaatc caaattgtcc cgggcggctc tcatcagccg ggagaaatgg ttgatatttt
     1381 taatatcatg tgctacgaca cccgccccga ccgccctgtg aaaaaggtcg gcgaaattcc
     1441 ccaagctgaa aaaacttata cctatttcca tatcggctac cccttcatga acgaccagca
     1501 ggtcatgatg ggcgagttca cctggagcgg acgggatgaa cttggaactc ctgaagggct
     1561 tttctatatc gaaaatctgg aagccctcgg tctggcgcga gcagccaccg cgcgggaagc
     1621 catcaaagtc atgggttccc ttgcagaaaa gtacggttat gcggacggcg gagaaacgct
     1681 tgtcatcgcg gatacgaagg agctttgggt attcgagatc tgcggccccg gactctggaa
     1741 caaagagtcc ggccggcccg gcgctcactg ggtagcgcag cgtgttcccg acgatgaagt
     1801 attcgtcgga gccaaccggg cgcgcatcgg cgtgatcgac ttcgccgaca ccgaaaacta
     1861 cattttctcc accgacatca ccaagctccc cgagcagatg gggtggtgg
//
LOCUS       DAOSWW010000557         1905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50684_length_1905_cov_3.602162, whole genome shotgun sequence.
ACCESSION   DAOSWW010000557 DAOSWW010000000
VERSION     DAOSWW010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50684_length_1905_cov_3.602162"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="PK442_10405"
     CDS             <1..1562
                     /locus_tag="PK442_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88106.1"
                     /translation="ANRLGPYHVPHAFASSQTEVLLPLAIHACADLMDLFFPTLELKA
                     EFEEQEEPRDAPDTEAIPKRFIEGTATMEHLKKKDLAWSAYDLTIQYSGPAELLVLEK
                     TKKIYSRKLHFNKGVLEKIETLEGALEKLPLRLFIAAEGFPLTEEEAFYEIKDGQSAV
                     IRVDAGSRRFENAPWELKNPAEVSIMPPRILMLELLEYTGDAEHEFEAVATPKGDYLF
                     EWDFGDGSEPIEDRPRPGEKSVRKHTYKGLKPGDLFSPKVILYGEKKKELAEDTISVS
                     VIKGEKRHFFENRTFWLTGSPFANDQSVVFPTVALSIVPSISGISGEIDPKSPEGKRL
                     ADMVEGMSKAGDYVKEQMIKEMAKMGVSLSDSQLAEAMKQALAMGGIDFSGSAMERRP
                     ILGSGYQQAGKPVTISLSVSLAPIPPVPVSMPDTPRLEGRVAVKKWFFTTGSAVSREV
                     AGSGGSASLSWTPEKGKTPESFALDLVMFYTLEFYKRGTMELWLDAGAPVRYENMSVS
                     FPVGVYFCPVR"
     gene            1595..>1905
                     /locus_tag="PK442_10410"
     CDS             1595..>1905
                     /locus_tag="PK442_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88107.1"
                     /translation="MKRACELQCDGIVFGGKKGAAHEDAFFCLFRRAWILIVCLLTLF
                     AFFPADAGAQEADPPALTDAPEGMLFRWVPPYYMTIPSGWKPMVERREGVGFFTGAHP
                     EA"
BASE COUNT          386 a          530 c          612 g          377 t
ORIGIN      
        1 gcgcgaaccg tctcggaccc tatcacgtgc cgcacgcctt cgcctcttcg cagaccgagg
       61 tgctgcttcc gctggccatc cacgcctgcg ccgacctgat ggatctcttc tttcccacgc
      121 tggagctgaa ggcggagttt gaagagcagg aggagccgcg cgacgcgccc gataccgaag
      181 cgattcccaa gcgttttatc gaaggaacgg ccacgatgga gcatctgaaa aagaaggacc
      241 tcgcatggtc cgcgtacgat ctgaccattc agtattcagg tcccgccgag ctgctggttc
      301 tggagaagac gaagaagatt tacagcagaa agctgcactt caacaaggga gtgcttgaga
      361 agatcgagac gcttgaaggc gctcttgaga agctgccgct ccgtcttttt atcgcagcgg
      421 agggattccc gctcactgaa gaagaggctt tctacgagat caaggacggg cagtccgcgg
      481 tcattcgggt agacgcgggg agccgcaggt ttgaaaatgc tccctgggag ctcaagaacc
      541 ctgccgaagt ctccatcatg cctccgcgta tcctcatgct ggagcttctt gagtatacag
      601 gcgacgcgga gcatgagttc gaggccgtcg ctactcccaa gggagactat ctcttcgagt
      661 gggatttcgg cgacggaagc gagccgatcg aggacaggcc gcgccccggg gagaaatccg
      721 tccggaaaca tacgtacaag ggcctgaagc cgggggatct gttctctccc aaggtgattc
      781 tctacggcga gaagaaaaag gagctggccg aggatacgat ttccgtctcg gtcatcaaag
      841 gggagaaacg gcatttcttc gagaaccgta ccttctggct gacgggctct ccctttgcca
      901 acgaccagtc cgtggtcttc ccgacggtcg cgctctccat cgtcccgtcc atctccggga
      961 tatccggaga aatagacccg aagagtccgg aggggaagcg tctcgcggac atggtcgagg
     1021 ggatgtcgaa ggcgggagac tacgtcaaag agcagatgat caaggagatg gcgaagatgg
     1081 gcgtttccct gagcgattca cagcttgccg aggcgatgaa gcaagcgctg gcgatgggcg
     1141 gaatcgattt ttcgggttcc gcgatggagc gccgcccgat cctcgggagc gggtatcaac
     1201 aggcaggcaa acctgtgacg atctcgctct ccgtgtcgct ggcgcccata ccccccgttc
     1261 cggtctccat gccggatact ccaaggcttg aaggacgtgt tgccgtgaaa aagtggttct
     1321 tcacgacggg ttccgccgtc agccgggagg ttgcaggaag cggcgggagc gcttcgctct
     1381 cgtggacgcc cgagaaggga aagacgccgg agtccttcgc cctcgacctc gtgatgttct
     1441 acacgctgga gttctacaaa cgggggacga tggagctttg gctcgatgcc ggggcgcccg
     1501 tgcgctacga gaatatgtcc gtgtcgttcc cggtaggggt ctacttctgc ccggtccgct
     1561 aatgagcatg atgatgaaag aggagggagt agtcatgaaa agagcctgcg aactgcagtg
     1621 cgatggtatt gtcttcggcg ggaagaaggg agcggcgcat gaggacgcct tcttctgcct
     1681 gtttcggcgg gcgtggattc tgattgtctg tctgctgacg ctgtttgcgt tcttccctgc
     1741 ggacgcgggg gcgcaggagg ccgatccgcc ggccctgaca gacgcgcccg aagggatgct
     1801 cttccgctgg gtccccccct attacatgac cattccttcc ggctggaaac ccatggtgga
     1861 gcgaagggag ggggtcggat tcttcaccgg cgcgcatccg gaggc
//
LOCUS       DAOSWW010000558         1903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50775_length_1903_cov_5.241342, whole genome shotgun sequence.
ACCESSION   DAOSWW010000558 DAOSWW010000000
VERSION     DAOSWW010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50775_length_1903_cov_5.241342"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..>1903
                     /locus_tag="PK442_10415"
     CDS             284..>1903
                     /locus_tag="PK442_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88108.1"
                     /translation="MSSTLRRNAVILCMVLFGVLFSWLVVERTDRKMRDDLLYQTRLG
                     VQTIDLDQVRALSGADEDVGKEEYHSLRRRLSAFREAADCRYVYLMQRRPVRDNPGAT
                     EVVFLVEAQNDGIETSPPSQPGDVYEEEYITEEFAALFDGGAPFVEGPVEDEWGIWVS
                     ALVPMVDHRTGKLLAVLGVDYDAKLWKWTVVSSSLLPVSLMLVLLLICIALFVLSRPV
                     DPMPRPVLKRLLPTMAVALLLLTSAAVLLLWRTYSDNLDERLNSLHASISAEFHLDLK
                     NELSLMESSIASLAEAPRLQWALFAGDADALFADWLSMFARLRREYGVRSFSFANEEF
                     RRLYSFAPGSDEKLFYDGPVVLKAKETGKIASGVEVCPSGNLVLRAVLPVSRAGELVG
                     YAIITKDVQGLLQKRSLRSGSHLALSLRKGLLDRAKWEKTCNPDRTTEWEQFPDCVIV
                     FSSMERLPPGIFSSGADDASDTVTVKERRRESVFAQKTWGVSVLPFEDFSGVIVGEFA
                     IMTDITADRSHLRSQVAMMWGIGGTIAAALLI"
BASE COUNT          362 a          508 c          593 g          440 t
ORIGIN      
        1 gaaggagagg ggccgtcttc tggtggcgcc tctggaggcg ctcgacggca cgccggttct
       61 ggatattaaa cccgtgctcc gggagtccgg accattcagc gaagcgtaga tatttttcag
      121 gaagtaagaa tgacatggaa gaaaagctga tatatatagt gtaaaataga tgtgttcgca
      181 aaggttcggt actcgataga cgcaacggaa aaagccgatg atttcggagt tttcccccga
      241 aggggagatg tgtgtgggag ttccctctgc tgcagaaaga acagtgtctt cgacgctccg
      301 ccggaacgcg gtcatcctgt gtatggtcct cttcggagtg ttgttttcat ggctggtcgt
      361 cgagcggacg gacaggaaga tgcgggacga tctgctctat cagacgcgcc ttggtgtgca
      421 gacgatagac cttgaccagg ttcgcgcgct ctccggcgcc gatgaggacg tgggaaaaga
      481 ggagtatcac agcctgcggc gccgtctttc ggcattccgg gaggctgcgg attgccgcta
      541 cgtctacctg atgcaacgca ggccggttag ggacaatccg ggggcgacgg aggtcgtgtt
      601 tcttgtcgag gcgcagaacg acgggatcga aacatcccct ccgtcgcaac caggagacgt
      661 ctacgaggaa gaatatatca ccgaggagtt cgccgctctc ttcgacggag gcgcgccttt
      721 cgtcgaaggc ccggtggagg acgagtgggg gatctgggtc tcggccctcg tccccatggt
      781 cgaccatcga acggggaagc tgctcgccgt cctcggggtg gattacgacg cgaagctctg
      841 gaaatggacc gtcgtctcgt cttcgttgct gccggtatcc ttgatgctgg tgcttctgtt
      901 gatttgtatc gcgctgttcg tcctctcccg gcctgtggat cccatgccgc gtccggtgct
      961 gaagagactg ctgccgacca tggcggtcgc cctgcttctc ctcacctccg cagcggtgct
     1021 tcttctgtgg cgtacgtatt ccgacaacct tgacgagcgg ctgaattccc ttcatgcgtc
     1081 gatttccgcc gaatttcatc tggacttgaa gaacgaactc tcccttatgg agtcctcgat
     1141 tgcctctctt gcggaggccc cccgtctgca atgggcgctc ttcgccggcg acgcggacgc
     1201 gctcttcgcc gactggctgt cgatgttcgc ccgcctgcgc cgggaatacg gcgtacgctc
     1261 tttttccttt gccaatgagg agttccggcg tctctattcc tttgcgccgg ggagcgatga
     1321 aaaactgttc tatgacgggc ctgtcgtgct gaaggcgaag gaaacgggaa agatcgcctc
     1381 cggtgtcgag gtctgtcctt ccggaaatct ggtgctgcgg gccgtcttgc cggtttcaag
     1441 ggctggagag cttgtggggt atgccattat aaccaaggat gttcaaggtc tcctccaaaa
     1501 aagatccctt cgttccggat cccacctggc gctttcactc agaaaaggcc tactggacag
     1561 ggcgaagtgg gaaaaaacgt gtaaccccga ccggacaacg gagtgggagc agttccccga
     1621 ctgtgtgatt gtcttttcat caatggagcg ccttcctccc ggtatttttt cttccggagc
     1681 agatgatgca tcggatactg taactgtaaa ggaacgaaga cgggagtcgg tcttcgccca
     1741 aaaaacatgg ggcgtctccg tgcttccgtt cgaagacttt tcgggcgtga ttgtcggaga
     1801 gtttgcgatc atgacggata ttacggcgga caggagccac ctgcggagcc aggtcgcgat
     1861 gatgtggggc atcggcggga cgatcgcggc ggcgctgctc att
//
LOCUS       DAOSWW010000559         1903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50800_length_1903_cov_2.752165, whole genome shotgun sequence.
ACCESSION   DAOSWW010000559 DAOSWW010000000
VERSION     DAOSWW010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50800_length_1903_cov_2.752165"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PK442_10420"
     CDS             <1..1270
                     /locus_tag="PK442_10420"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOO88109.1"
                     /translation="MFIDEAKEPFSRDTQGVSQQRAAHGRTSSGPLLVFILSDSIRGH
                     LSQSRGIAGWLSRFTGADIVEIDVPRITGKRRVELLKVKGRRLPKMNSCDILVWLDET
                     GGSVLFDTMKNELAARKLTGKDALFLAAGGSAAPFALALAKMTRGKSCVLMTPSALGT
                     APFDFAVVPAHDRPRFTPNLLATLGAPNAIFPDELERKGWELAELYPPVESAEQRWGL
                     LVGGDDANYAVTADWVRRVISPILSAAGKEGADLYITTSRRTAPEAEEALTRLAAGRS
                     HVRMLLLASRDEFNPVAGMLGLCSRLFVTEDSVSMVSEAITAGREVFLLRTDRKGKLR
                     VAMQDLTAWLVRRRLLPPGVLWGKPRFDALFDVLERRGFLKEMQPRALRRSSSGGASL
                     HADGPVLNEARRAAEWIVERWGETGKDNRK"
     gene            1316..>1903
                     /locus_tag="PK442_10425"
     CDS             1316..>1903
                     /locus_tag="PK442_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88110.1"
                     /translation="MNILFCSDAMIVDGVTSFVFHLATALSEGGHRVAVLGRWGGKGF
                     QSRLRTYGVEVISIPSVTVGNFWFDKKAREFAPDVLVTDSRRSFPLAVRLKRATGAKI
                     FTFFLDNLEKTDKKGRDVDSLVRWSDAWLSAEPPLLEKLEEIPTPFPKCLFQRPLTGL
                     LTPTPIPARDPFRILCFGRISGYKSAGPLKLLQEAP"
BASE COUNT          331 a          596 c          611 g          365 t
ORIGIN      
        1 catgttcatc gacgaagcaa aggaaccgtt ctcccgcgat acgcagggtg tttcgcagca
       61 gcgcgcggcg catgggcgga catcatccgg gcctttgctc gtctttatcc tgagcgactc
      121 gatacgcgga cacctttcgc aaagcagggg catcgccggg tggctctcgc gcttcaccgg
      181 cgccgacatc gtcgagatcg acgttcccag gatcaccgga aaacggcggg tggagctgct
      241 caaggtgaag gggcgccgtc tgccgaagat gaactcgtgc gatatcctcg tctggctcga
      301 cgagacggga ggctccgtgc tcttcgacac gatgaaaaac gaactcgccg cccgcaagct
      361 caccggcaag gacgcgctct tccttgctgc gggagggagc gcggcgccgt ttgccctcgc
      421 gctcgcgaag atgacgcggg gcaagtcgtg cgttctgatg accccctcgg cgctcggtac
      481 ggcgcccttc gatttcgcgg tggtgcccgc acacgaccgc ccgcggttca cgcccaactt
      541 gctggcgacc ctgggagcgc ccaatgcgat ctttcccgat gaactggagc ggaaggggtg
      601 ggaacttgcg gagctctatc ctcccgtgga gagcgctgag cagcgatggg gtctcctcgt
      661 cggaggggac gacgccaact acgccgtcac ggcggactgg gtgcggcgcg tgatctcccc
      721 gattctctcg gccgcaggaa aagaaggcgc ggatctttac atcactacgt cgcggcggac
      781 cgcccccgag gcggaagaag cgctgacgcg gcttgccgcc ggacgctcgc acgtacgcat
      841 gcttcttctc gcgtcgcggg acgagttcaa cccggtcgcc ggaatgctcg ggctctgctc
      901 gcgccttttc gtcaccgagg actccgtctc gatggtttcc gaggccatca ccgccggaag
      961 agaggtcttc cttctgcgaa ccgaccggaa ggggaagctg cgcgtcgcga tgcaggatct
     1021 caccgcatgg ctcgtacgga ggcgactgct gccgccgggg gtgctttggg gcaaaccgcg
     1081 cttcgacgcc ctcttcgacg tactggaacg ccgtggattt ctgaaggaga tgcagccccg
     1141 cgcccttcga cgctcttcct cgggaggcgc gtcgctccac gccgacggcc ccgtcctcaa
     1201 cgaggcgcgg cgggcggcgg agtggatcgt cgaacggtgg ggagaaacgg gaaaggacaa
     1261 cagaaaataa acgcgcggcg tgtgtacgcg ccgataaaag gagctccatt ctcggatgaa
     1321 catacttttt tgttcggacg ccatgatcgt cgacggcgtc acttcgtttg tttttcatct
     1381 tgcgactgcc ctcagcgaag gagggcatcg ggtcgccgtt ctcggacggt ggggcggcaa
     1441 ggggtttcaa tcccgcctcc gcacgtacgg cgtggaggtg atctccattc cctccgtgac
     1501 agtcggaaac ttctggttcg ataagaaggc tcgtgagttt gcgcctgatg ttctggtcac
     1561 cgattcccga cgctcctttc cccttgccgt gcggctcaaa cgagcgacgg gggcgaaaat
     1621 tttcaccttc tttctggaca acctggaaaa aacggacaag aaggggcgcg acgtcgattc
     1681 cctggtgcgc tggagcgacg catggctctc tgcagagcct ccgcttttgg agaagctgga
     1741 ggagataccg actccgtttc cgaagtgcct cttccagcgc cctctcaccg gtctgcttac
     1801 cccgacgccg attcctgcgc gcgacccgtt ccggatcctc tgtttcggca ggataagcgg
     1861 gtacaagtcg gccgggcctt tgaagctgct ccaggaggcg ccg
//
LOCUS       DAOSWW010000560         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_50874_length_1901_cov_4.447454, whole genome shotgun sequence.
ACCESSION   DAOSWW010000560 DAOSWW010000000
VERSION     DAOSWW010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50874_length_1901_cov_4.447454"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..822
                     /locus_tag="PK442_10430"
     CDS             175..822
                     /locus_tag="PK442_10430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO88111.1"
                     /translation="MFSCVIFDFDMTLVDSSYAIRDTMNMVAGREGLPKVTREDVLSV
                     IGLPIRDSWLKVWGRFDERWLADFREMFVQQEFAGIFPFSGTKQVLETLAARGVSLGI
                     ASNRQRPGGPLKAVGLESYFSSVVGMDDVEKGKPAPDMLLKGMADLGGTPATTLYVGD
                     TADDMTAAKDAGIRSVGLTTGNFTPEALHAAGAWKVYDAVEEILPLFGTEGGRSE"
     gene            815..>1901
                     /locus_tag="PK442_10435"
     CDS             815..>1901
                     /locus_tag="PK442_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOO88112.1"
                     /translation="MSNAKNRLAKEKSPYLLQHAENPVDWYPWGEEAFAKARTEDKPL
                     FVSIGYATCHWCHVMERESFDDGEVASLLNDACVPVKVDREERPDVDGAFMAVCQMMN
                     GNGGWPLNTFITPQGLPFFASTYLPKRGGGKYPGLVDVVPRVKWLWKTQREQVEQSAK
                     SIRDSLLKEEAPSSGSLPGAAQFKAAYDGLRAGYDREFGGFSKEPKFPMPSHLLFLLR
                     YWKRFGEENAWSMAESSIERIWEGGIHDHLGGGIARYATDRRWLLPHFEKMLYDQALL
                     LHVLSEYQNERGNPLFGRFADDLAGFVLREMTSPEGGFYSAFDADSEGEEGKFYLWTE
                     DQVRAVLTPEESGIFICAYGVRKGGNVK"
BASE COUNT          361 a          606 c          595 g          339 t
ORIGIN      
        1 agaaggcgct cgccgacctg cttcccgaac gggcgctcca gggaaacctg aaggtgctca
       61 aggggtagcc aagccggctg tgcgcaaagg ccgctcctgc tggggcggcc tttgcttttc
      121 tcctgcgcat ggagtaccat ttccctggac gattcgagaa aaggagtgga ttcgatgttt
      181 tcatgcgtca tcttcgactt cgacatgacc cttgtggaca gcagctacgc catacgggac
      241 acgatgaata tggtcgccgg gcgggagggg ctccccaaag tcacgcggga ggacgtgctc
      301 tccgtcatcg gtctgccgat acgcgattcc tggctgaagg tctggggcag attcgacgag
      361 cgctggctcg ccgacttccg cgagatgttc gttcagcagg agttcgcggg aatctttccc
      421 ttctccggga cgaaacaggt gctcgaaacg ctcgcggctc gcggcgtctc cctcggcatc
      481 gcctccaacc gtcagcgacc cggaggcccc ttgaaggccg tcgggctgga gagctacttc
      541 agctccgtgg tgggaatgga cgacgtggag aagggcaagc ccgcccccga tatgctcctc
      601 aaggggatgg ccgacctcgg cggcacgccc gcgaccactc tctacgtcgg cgacaccgcc
      661 gacgacatga ccgccgccaa agacgccgga atccgttcgg tcggactgac caccggcaac
      721 ttcacgcccg aagcccttca cgccgcgggc gcgtggaagg tctacgacgc cgtggaagag
      781 attctccccc tcttcggaac ggagggcggc cgcagtgagt aacgcaaaaa accgtctcgc
      841 aaaggaaaaa tccccctacc tgctccagca cgcggagaac cccgtcgatt ggtacccctg
      901 gggagaggaa gccttcgcga aagccaggac ggaggacaag ccgctcttcg tctccatcgg
      961 gtacgccacc tgccactggt gccacgtcat ggagcgcgag tcgttcgacg acggcgaggt
     1021 tgccagcctt ctcaacgacg cctgcgtccc ggtgaaggtc gaccgcgagg agcgccccga
     1081 cgtcgacggc gccttcatgg ccgtttgcca gatgatgaac ggcaacggcg gctggccgct
     1141 caacaccttc attaccccgc aggggcttcc cttcttcgcg tcgacttacc ttcccaagcg
     1201 gggaggcggc aaataccccg gccttgtgga tgtcgtcccg agggtgaaat ggctgtggaa
     1261 gacccagcgc gaacaggtgg agcagtccgc gaagagcatc cgcgactccc tgctgaagga
     1321 agaggctccg tcttcaggct cccttccggg agcagcccag ttcaaagccg cctacgacgg
     1381 cctccgcgcc gggtacgacc gggagtttgg aggcttctcc aaagagccga agttccccat
     1441 gccgtcgcac ctcctcttcc tccttcgtta ttggaaacgt tttggcgaag agaacgcatg
     1501 gtccatggcc gaaagctcca tcgagcgtat ctgggagggc ggcattcacg accacctcgg
     1561 cggcggcatc gcccgctacg ccaccgaccg gcgatggctg ttgccgcact tcgagaagat
     1621 gctctacgac caggcgcttc tgctgcacgt gctttcggag tatcagaacg agcggggcaa
     1681 tccgttgttc ggccggtttg ccgacgatct cgcaggattc gtcctccgtg agatgacctc
     1741 tccggagggg ggattttact cagctttcga cgccgacagc gaaggtgagg agggaaagtt
     1801 ctacctctgg accgaagacc aggtgcgggc cgtcctgacc cccgaggagt cgggcatctt
     1861 catctgcgcc tacggcgtgc ggaagggggg caacgtcaag a
//
LOCUS       DAOSWW010000561         1898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51032_length_1898_cov_2.525773, whole genome shotgun sequence.
ACCESSION   DAOSWW010000561 DAOSWW010000000
VERSION     DAOSWW010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51032_length_1898_cov_2.525773"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..445)
                     /locus_tag="PK442_10440"
     CDS             complement(2..445)
                     /locus_tag="PK442_10440"
                     /inference="COORDINATES: protein motif:HMM:NF018008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO88113.1"
                     /translation="MDKKELESLVRKIILEIAGEGCGGSFEKTADPSGILSVSIPTVK
                     PAPFDTGKKGDQVYLTDVVTLQESPRLGCGVMEMEESAFDWTLRYDEVDYIIEGTLEI
                     LIDGRKVTGKKGDMIFIPANSSITFSAPCFARFLYVTYPADWASQ"
     gene            complement(510..1634)
                     /locus_tag="PK442_10445"
     CDS             complement(510..1634)
                     /locus_tag="PK442_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HOO88114.1"
                     /translation="MGINDIILYIMTGFMVLGALDKAFLNSRLGFGEKFEEGFMAMGS
                     LMLAMGGVITLAPLLAAILKPVIVPLYQAVGADPAMFATTLLANDMGGYPLAMQMAEN
                     PAVGRFAGIILGAMMGPTIVFSIPVALGIIEKKDHPALAKGILLGIVTIPFGCLAGGL
                     VMLSSEFTVGMMLVNLIPVVIFSALIATGLKMIPNAMIKGFTYFGKGVVTVITIGLAM
                     GIIEALSPFRFFAEGSPMALAPLSDSIATIGAIAIVLAGAFPMVMFITKVFKQPLLVF
                     GRKLGMGDVAAAGMVATLANNIPMFTLMKDMDERGKVINVTFSVSAAFVFGDHLGFTA
                     GVERGLILAMIVGKLVGGITAVILACKVTPANAPAPAKKK"
     gene            complement(1771..1893)
                     /locus_tag="PK442_10450"
     CDS             complement(1771..1893)
                     /locus_tag="PK442_10450"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOO88115.1"
                     /translation="MLVSQGSSARTVFAGGDVPVDAVIVGIIDSVEVDESLLEY"
BASE COUNT          375 a          595 c          587 g          341 t
ORIGIN      
        1 gttactgcga cgcccagtcc gccgggtagg tcacgtagag aaaacgcgcg aagcagggcg
       61 cgctgaaggt gatcgacgag ttcgcgggga tgaagatcat gtcgcccttc ttgcccgtga
      121 ctttccgccc gtcgatcaga atctccaggg ttccttcgat gatgtagtcc acctcgtcgt
      181 aacgaagcgt ccagtcgaag gccgactcct ccatctccat gacgccgcac cccaggcggg
      241 ggctctcctg caacgtgacc acgtccgtga ggtagacctg gtcccctttc ttgcccgtgt
      301 cgaacggcgc gggcttcacg gtggggatgc tcaccgagag gatgccgctc gggtccgccg
      361 tcttctcgaa ggagcccccg cacccttcgc ccgcgatctc cagaatgatc ttccgaacga
      421 gcgattcaag ctcttttttg tccatgcata cacaccacgc tttctacgaa cggtcctccg
      481 gagggcggcc gccctccgga ggaagcgcac tacttctttt tggccggggc cggggcgttg
      541 gccggcgtca ccttgcacgc aaggatgacg gccgtgatgc caccgaccag cttgccgacg
      601 atcatggcga ggatgaggcc gcgctcgacg cccgccgtga agccgaggtg gtcgccgaag
      661 acgaaggccg cgctcaccga gaaggtcacg ttgatgacct taccgcgctc gtccatatcc
      721 ttcatcaggg tgaacatcgg gatgttgttc gccagcgtcg ccaccattcc cgccgccgcc
      781 acgtcgccca tgccgagctt cctgccgaag accaggagag gctgcttgaa gaccttggtg
      841 atgaacatga ccatcgggaa cgcgcctgcg agcacgatgg cgatagcgcc gattgtcgcg
      901 atggaatcgg agagaggagc aagcgccatc ggcgatcctt cggcgaagaa gcggaacgga
      961 gaaagcgctt cgatgatgcc catcgccaat ccgatggtga tgaccgtgac gactcccttg
     1021 ccgaagtagg taaacccttt gatcatcgcg ttcggaatca tcttgagccc cgtcgcgatg
     1081 agcgcggaga agatgaccac gggaataagg ttgacgagca tcataccgac ggtgaactcg
     1141 ctgctcagca tgacgaggcc gcccgcgagg cagccgaagg ggatggtcac aataccgagc
     1201 aggattccct tggcgagcgc cgggtggtcc ttcttctcga tgatgccaag agccacgggg
     1261 atggagaaga cgatggtcgg gcccatcatg gcgccgagga tgatgcctgc gaagcgcccg
     1321 acggccgggt tttccgccat ctgcatcgcg agcggatagc cgcccatgtc gttggcgagc
     1381 agcgtggtgg cgaacatcgc cggatccgcg cccacggcct ggtagagcgg cacgataaca
     1441 ggcttcagga tagcggcgag cagcggagca agggtgatga cgccgcccat cgcgagcatc
     1501 agggagccca tcgccatgaa gccctcctcg aacttctcgc cgaagccgag acggctgttc
     1561 agaaacgcct tgtcgagcgc gcccaaaacc atgaatccgg tcatgatgta gagaatgata
     1621 tcgttgattc ccatgcagtt cacctccgaa tagaatggcg gttccggttc ttttccggta
     1681 ctcaaagtgc cgatggtgca tcctcgcgcc cctcggattt cgagcggcgc ggcgataccc
     1741 tgcccggtgt gtctcgggcg caatgagccg ctaatactcc agcagactct cgtccacctc
     1801 cacggagtcg atgatgccga cgatgacggc gtccacagga acgtcgccgc ccgcgaagac
     1861 ggtccgcgcc gagctgccct gcgagacgag cacgtcgt
//
LOCUS       DAOSWW010000562         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51199_length_1894_cov_3.219685, whole genome shotgun sequence.
ACCESSION   DAOSWW010000562 DAOSWW010000000
VERSION     DAOSWW010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51199_length_1894_cov_3.219685"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PK442_10455"
     CDS             <1..470
                     /locus_tag="PK442_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88116.1"
                     /translation="LGKDALVARQLSARGGTVYCSYNGDSVGISGRGALYSVGTIFVN
                     GSNEYSISKINTTDTAELCEISVENKRALGISVNIPGAELQILQIRCQKGMLFCRVFD
                     TSVVEKLQFPAAIFSAPKFEDMLNNNPIHLSKAAVALGATADMTGRELVKLFS"
     gene            761..1462
                     /locus_tag="PK442_10460"
     CDS             761..1462
                     /locus_tag="PK442_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88117.1"
                     /translation="MDKKTELIHRLRVDFLSKYGSSELYHEMDSVVNEMTGYLSHLLN
                     TKEDIELFFQCLPQILSEMPRESEETPLGHAPSYSAGVFETDARNAVSMSAFLKPFIR
                     ESVEEAGSLAYFHEYHPRTDNQPAHSNEGAKLQAVFNFQGVEELKKLCVRTGVNYERL
                     RKECCYHGEIHGVPVFVSYALDESSKKGRLELNILDPENPYTITETSRAMARNVDSFL
                     KAVFNKKKRRFANKS"
     gene            complement(1693..>1894)
                     /locus_tag="PK442_10465"
     CDS             complement(1693..>1894)
                     /locus_tag="PK442_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOO88118.1"
                     /translation="TLLLAYTGSYTSLLLVFVAQGIPLANILNMQYVSAEILHTLVGS
                     FGLVTVAPFTALASGFLFVKKS"
BASE COUNT          481 a          503 c          496 g          414 t
ORIGIN      
        1 ggcttgggaa agatgcgctg gtggcgcggc agctttcagc gcgaggcgga accgtctatt
       61 gttcgtacaa cggcgacagc gtcggcatca gcggaagagg cgcgctctat tccgttggaa
      121 ccatttttgt gaacggatcg aacgagtatt ccatatcgaa aatcaatacg acggatacag
      181 ccgaactctg cgaaatttcc gtggagaaca agcgcgcgct gggcatttca gtaaacattc
      241 ccggcgccga actccaaatt ctacagattc gatgccaaaa agggatgctt ttttgcagag
      301 ttttcgatac gtcggttgtc gaaaaacttc aattcccggc ggcaatcttt tccgctccca
      361 agttcgaaga catgctgaac aacaacccca tccatctcag caaggccgcc gtagctttgg
      421 gcgcaacagc cgatatgacc ggacgcgagt tggtgaagct gttcagttga gtgatcgctc
      481 tacaaaaccg gacgggaaca acacattcgt atttgagcga aatggaattt cctgttgaaa
      541 tgaggcgaag ggacggccgc ccgagagcgc gtcaaaccca tgccttcacc gcccagggct
      601 tgcaatagcg cccgggcaca gcgcatatac tcttgcattt gttccgaaac gaaacgatac
      661 tctgcttgct cggtcggaga acgacgcttg ctctgcagga tattctgaat caaaaagtaa
      721 aacggctttc gcgaagagag tcctgaaggg gggcagggta ttggataaaa aaacggaatt
      781 gattcaccgg cttcgcgtcg attttctttc aaaatacgga agttcggaac tctatcatga
      841 gatggactcc gttgtcaacg aaatgacggg ctatctctcc cacttgctga atacaaaaga
      901 agatatagaa ctcttttttc aatgccttcc gcaaattctt tcggaaatgc cccgcgagag
      961 cgaagagact ccgctcgggc acgccccctc ctattcggcg ggtgtttttg aaacggacgc
     1021 tcgaaacgcg gtttcgatga gcgcatttct caaaccgttc attcgtgaga gcgtggaaga
     1081 agccgggagt cttgcatatt ttcatgagta tcatccgcgg acggacaatc agcccgccca
     1141 ctcgaatgaa ggcgccaaat tacaggccgt tttcaacttt cagggtgttg aagagctgaa
     1201 aaaactgtgc gtcaggacag gggtgaacta tgaaagactg agaaaagaat gctgttacca
     1261 tggagagatt cacggcgtcc ctgttttcgt gtcgtacgct ttggacgagt ccagcaaaaa
     1321 ggggcgcctt gaactgaaca ttcttgaccc tgagaatcca tatacaatca ccgaaacttc
     1381 gcgcgcaatg gcccgaaatg tcgacagttt cttaaaagcc gttttcaata agaagaagcg
     1441 gcgcttcgcg aacaagtcat gaaccgtgtt tcgtcgaaac cgaagcggat acgttgcaga
     1501 agaggatcgc gcgcgacatg accggcacgc caaggattca ttggggccaa ctgaaggact
     1561 atgatacggc gactctcccg ggaagcgctc cccgcatccg ctcctgttcg tacttccgct
     1621 tcccttttcc gaaacctccc tcgacttacg ccaagacacg ctctccgatg cacggactga
     1681 gcgcgtcgga tttcagctct tttttacgaa cagaaaaccg ctcgccaggg cggtgaaggg
     1741 ggcgaccgtc accaggccga agctccccac cagcgtgtgc agtatctccg ccgagacgta
     1801 ctgcatgttc aatatgttcg ccaacgggat tccctgcgcg acgaagacca gcagcaggct
     1861 cgtgtagctc cccgtgtacg ccagcagcag cgtc
//
LOCUS       DAOSWW010000563         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51212_length_1894_cov_2.460033, whole genome shotgun sequence.
ACCESSION   DAOSWW010000563 DAOSWW010000000
VERSION     DAOSWW010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51212_length_1894_cov_2.460033"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1894)
                     /locus_tag="PK442_10470"
     CDS             complement(<1..>1894)
                     /locus_tag="PK442_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO88119.1"
                     /translation="LVSDLSTTGMTVISQARKELAFLEGRVQVLDLFAFSGEELQDAL
                     SALPPETVVLMLVYFQDEQGNFISPSRGVALVKDACAFPLFGLWSMMPEAGAFGGSVL
                     QARRHGEKAAEIALRVLAGVPPSAIPAETDTEYVPMFDFGELKRFRLSRSDLGQSSVV
                     LNEPERISSGSWKIIIINAVVVLASIGYVALLLVNERRRKRAAKEIALRKSMWEGLFR
                     NAPDAYVVFGDDNTVLNVNEKFCEVFGYEYGEAIGRDIDSLVAKGTDAEEEAAEISRK
                     VLNGEAVYSEGVRRKKDGNTIHVGIQGVLFPVPEGRALGYGIYSDISERRRNEEEMNR
                     RLLSEAAISSISARLLKESGLIGIPPSLEEMASFTGAKEGFLIEFSTPGEIVRELLFS
                     EGRAVWSASPHISEVFPESDLRTITQYLWEKGRLLLDERLPAPLCEVDRLWELKRLFG
                     APLLILPIYSERMVSGWLGLQLPESPLLSSHESFLGMFCDLVGGVLLQQKRRSMTSER
                     ARILDGTSRGIVEILGRTLAMKDPYTVGHQFEVARLARAMAERGGYDEAFLERVYYAG
                     LVHDLGKIVIPSSILSKPGRLNTVEFEIIKNHALHGWEILSSTEFPWPLAEIVVQHHE
                     RLDGSG"
BASE COUNT          393 a          607 c          531 g          363 t
ORIGIN      
        1 tagccgctgc cgtcgagacg ttcgtggtgc tgcaccacga tttcggcaag cggccacggg
       61 aattccgtcg atgagagaat ctcccatccg tgcagggcgt ggttcttgat gatctcgaac
      121 tccaccgtgt tcaaccgccc tggttttgag aggatggacg acggaatcac gatcttgccc
      181 aggtcgtgga caagaccggc gtaatacacc cgttccagaa aagcctcgtc ataccctccg
      241 cgttccgcca tcgcccgggc aaggcgcgcc acctcgaact gatgccccac ggtgtacgga
      301 tccttcatgg cgagcgtccg cccgaggatc tcgacgattc cgcgcgaagt accgtcgagg
      361 atgcgcgcgc gctccgacgt catgctccgc cgcttctgct gcagcaggac tccgccgaca
      421 aggtcgcaga acattcccag gaaagattcg tgggaggaaa gaagaggact ctctggaagc
      481 tgaagcccga gccagccgct caccatccgt tccgagtaaa tggggagtat caggagaggg
      541 gctccgaaga gccgcttcag ttcccaaagc cggtccacct cgcagagcgg cgccggcagg
      601 cgttcgtcca gcagcagccg ccccttctcc cagagatact gcgtgatcgt gcgaaggtcg
      661 gattcaggga agacttcgga gatatgcggc gaagcgctcc ataccgcccg cccttccgag
      721 aagagcagct cgcggacaat ctcgcccggc gtggagaatt cgataagaaa tccttccttc
      781 gctcccgtaa acgaagccat ctcttcaagc gacgggggaa tgcctataag tccgctctct
      841 ttcaggaggc gcgcggaaat agaggaaatt gcggcctcgg agagaagacg ccggttcatt
      901 tcctcttcat tgcggcgccg ctccgagatg tcgctgtaaa taccgtaccc cagcgcgcgg
      961 ccctcgggca cggggaagag caccccctga atacccacat gaatagtgtt tccgtccttc
     1021 tttcttcgca ctccttcgga atagaccgcc tcgccgttca ggaccttccg ggagatctcc
     1081 gccgcttcct cttccgcatc cgtccccttt gcaaccagac tgtcgatatc tcgtccgatc
     1141 gcctcaccat actcgtaacc gaagacctcg cagaatttct cgtttacatt caggactgtg
     1201 ttgtcgtctc cgaagacaac gtatgcgtcc ggagcgttcc tgaagagccc ctcccacatg
     1261 gatttccgga gcgcgatctc ttttgccgct ctcttccgcc tgcgttcgtt gaccagcagc
     1321 agcgcgacat accctatgga cgcgagtaca acgacagcgt tgatgatgat gattttccag
     1381 ctccccgaag aaatacgctc cggctcgttg agcaccaccg aactctgccc cagatcactt
     1441 cgcgagagcc ggaagcgctt cagctctccg aagtcgaaca tcggcacgta ctccgtatcg
     1501 gtttcagcgg ggatagccga gggggggacg cccgcgagca cgcgaagcgc aatctccgcg
     1561 gccttttctc cgtgacggcg ggcctgcaga acgctgccgc cgaatgctcc agcctcgggc
     1621 atcatgctcc agagaccgaa gagcgggaag gcgcatgcgt cttttacaag ggcgacgccg
     1681 cgcgacggag agatgaaatt cccttgttca tcctgaaagt acaccagcat gagcaccaca
     1741 gtctcgggcg gaagagcgga aagcgcatcc tggagttcct cccccgagaa tgcgaagagg
     1801 tcgaggacct ggacgcggcc ctccaagaaa gcaagttcct tccgcgcctg gctgatcacc
     1861 gtcattcccg tggtggagag atcgctcacg agcg
//
LOCUS       DAOSWW010000564         1890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51407_length_1890_cov_3.138965, whole genome shotgun sequence.
ACCESSION   DAOSWW010000564 DAOSWW010000000
VERSION     DAOSWW010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51407_length_1890_cov_3.138965"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PK442_10475"
     CDS             complement(<1..1040)
                     /locus_tag="PK442_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOO88120.1"
                     /translation="MKILRIVAILLLAMGAAFAGAAFKVKTFVEPQPQTIRESISFTE
                     ESGSINLLLIGIDDVDGGRRADAIAYATIDIDNKIVRLMSIPRDTRVQLPKRGWEKIA
                     HAYAYGGVELLRETVVNYLGMPINYYVLVNYDTFPSIVDLIGGVEVDVPKRMVYNDYS
                     GKLFINIPKGLQVLDGKNALHYVRFRHDALGDIGRVKRQQDFIKAVLAKLQSPYMIPK
                     IPDLANKAIELVNSDMTPAQGLQLASYLVSMPSENIHFFTMPGKAAYISNISYWVGDI
                     TAASALLTGVPEGHGTLSGDVAKEETDMGTLDLSTTDVPALISSINTPVAVLNGAGVS
                     GLGKTMATALQK"
     gene            complement(1063..1656)
                     /gene="nadD"
                     /locus_tag="PK442_10480"
                     /pseudo
     CDS             complement(1063..1656)
                     /gene="nadD"
                     /locus_tag="PK442_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
     gene            complement(1714..>1890)
                     /gene="cgtA"
                     /locus_tag="PK442_10485"
     CDS             complement(1714..>1890)
                     /gene="cgtA"
                     /locus_tag="PK442_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HOO88121.1"
                     /translation="YDFEQEDALPKFARLVKKFRIEERLEEMGAVKGDKVFIGDHEFD
                     FEPDMAGASRSENR"
BASE COUNT          465 a          515 c          426 g          484 t
ORIGIN      
        1 atcttttgca atgccgtcgc cattgtcttt ccaagacctg agacgccggc gccatttaaa
       61 acagccacag gcgtatttat tgaagagatc aatgccggaa catccgttgt agagagatca
      121 agcgttccca tatcggtctc ttctttcgcc acatctccgg atagcgttcc gtgtccttcc
      181 ggaacccccg ttaacagcgc ggaggcggct gtgatgtcgc caacccagta gctgatattc
      241 gagatgtacg cggccttccc gggcatggta aaaaaatgta tgttttcact cggcatcgaa
      301 acaaggtagg acgcaagctg taacccctga gcaggcgtca tatccgagtt caccaactca
      361 atcgctttat tcgccaagtc aggtatcttc ggtatcatgt aagggctctg caatttcgcc
      421 aaaacagcct ttataaagtc ctgctggcgc ttgacacgac caatatcccc cagcgcgtca
      481 tgacggaatc gaacgtaatg gagtgcattc ttcccgtcga gcacctgcaa accctttggg
      541 atattgataa aaagtttgcc cgaatagtca ttgtagacca tgcgttttgg cacgtcgact
      601 tcgacgcctc cgataagatc aactatagag ggaaaggtat cataattcac tagaacgtag
      661 tagttgatcg gcatgccaag gtagtttaca actgtttcac gtagaagttc gacccctcca
      721 tatgcatacg catgggcaat tttttcccag cctcttttcg gcagctggac acgcgtatcg
      781 cgcggtattg acatgagacg aacaatctta ttatcgatat cgatggttgc atatgcgatc
      841 gcatccgcgc gtcgccctcc gtcgacatcg tcaataccga tgaggaggag atttatagat
      901 cccgactcct ccgtaaaaga gatggactcc ctaattgtct gcggttgagg ctctacgaac
      961 gtcttcacct tgaaggctgc ccctgcgaaa gccgctccca ttgccaagag aagaattgcc
     1021 actatgcgca gtattttcat tgtttatcgc cctccagttt ttccggtaga gtcctttctt
     1081 gtatatatac tgctccaccg tttcaggaag aaggtatttt atgctcctcc ccgtctctat
     1141 ccgctggcgg atttccgtac tcgaaataga gaggagcgga atttcgagtg gaatgattgc
     1201 atcccggaga gtcggcggaa gcatatccaa cgcgttctgg gcatatccgg gccgattaac
     1261 cgccacaata cggcataact tcgggagttc agcgtattct ttccatgtcg ccatgcttaa
     1321 aacagcatcc agccccgtaa tgaagaaaaa ctctacagtt tcgggagcat accagtgacg
     1381 catctcccga agagtatcga cagtatgact cggttccttt ctgtcgatct cgatccgcga
     1441 aggcgtaaaa ttaggattct ccaacgtcgt taagagggtc atgatatacc ggtcttcaca
     1501 gaaagcaacg cgacgctgac gtttatgcgc cgggtctcca gcaggtacga aaatcacctc
     1561 atcaagaccg acagcagtcc ggctttcttc cgcagctaat aggtggccga agtgaattgg
     1621 atcgaaagtc ccgcccataa tgcctattct tcgcacatca ttgcgtacag aatacgacac
     1681 gtcgtcaact ccttcgcttc cgactcccct gcttcatctg ttctccgaac gagaagcccc
     1741 tgccatatcc ggttcaaagt cgaattcgtg atcgcctata aataccttat caccttttac
     1801 tgcgcccatt tcctcaagac gttcctcgat gcggaacttt ttcacgagcc gggcaaactt
     1861 aggaagcgca tcctcctgtt caaagtcata
//
LOCUS       DAOSWW010000565         1890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51434_length_1890_cov_2.331880, whole genome shotgun sequence.
ACCESSION   DAOSWW010000565 DAOSWW010000000
VERSION     DAOSWW010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51434_length_1890_cov_2.331880"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PK442_10490"
     CDS             complement(<1..1131)
                     /locus_tag="PK442_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88122.1"
                     /translation="MKRMKACCLAIAAAIALCLTGFAPRAEATIWKVTRTDDNPSNPA
                     EGSLRHAVNNAWNNDFITFESTGMTIDLVEELSISKTLTIVGSGTASPTTIRQTAPGK
                     RVFSIAPGVFGPRFEYLIIRGGNAAESGGAILNRGYSLTLYRCTLDGNSAANYGGGID
                     NHGKLTLNIVTVKNNSASSGGGIKNRTGSSCDMYMSTITGNTPDQIVGAYTTDGHCTI
                     GDSAGTASVAFGGIPEGVSPKPRKTSGEADVTTVENNLKKPESSLFAAVKSALAADLG
                     GLTGDVSAGLSGMNATLYDAFAYEDVPLADASGKGELVVEFTASWPQNARYYAALAEY
                     EETAARTKATREVASLAIKGYVLPERGVQFEIKPGQPLPDGVT"
     gene            complement(1342..1497)
                     /locus_tag="PK442_10495"
     CDS             complement(1342..1497)
                     /locus_tag="PK442_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88123.1"
                     /translation="MTQEQKKVGAKLEHDLKPEELKKKQKVGELDDTELDGISGGEGM
                     DTGKIIP"
     gene            complement(1680..>1890)
                     /locus_tag="PK442_10500"
     CDS             complement(1680..>1890)
                     /locus_tag="PK442_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88124.1"
                     /translation="WRNVVEDNGPFDHNRQIGAVTFRAASIRAEARTAAAASGGSGCS
                     TSAASPLALLFGAPLLLLPRRKRGN"
BASE COUNT          303 a          620 c          557 g          410 t
ORIGIN      
        1 cgttacgccg tcggggagcg gctgccccgg cttgatctcg aactgtaccc cccgctcggg
       61 gaggacgtac cccttgatcg cgagcgacgc gacctctcgc gtagctttgg tccgcgccgc
      121 tgtttcttcg tattccgcga gcgccgcgta gtagcgggcg ttctgcggcc acgaggccgt
      181 gaactcgacg accagttcgc ccttgcccga cgcgtcggcg agcggcacgt cctcgtaggc
      241 gaaggcgtcg tacagcgtcg cgttcatccc cgaaagcccc gccgaaacgt cgccggtcag
      301 tcctcccagg tccgccgcga gggcgctctt caccgccgcg aagagcgagc tttccggttt
      361 cttcaggttg ttctccaccg tcgtgacgtc ggcttcgccg ctggtcttgc ggggtttggg
      421 agaaacgccc tcggggatac cgccgaaagc gacagaggcc gttcccgccg agtcgccgat
      481 ggtgcagtgc ccgtccgtgg tgtaggcgcc gacgatctgg tcgggcgtgt ttcccgtgat
      541 cgtcgacatg tacatgtcgc aggagcttcc cgtccggttc ttgatcccgc cgcccgaaga
      601 cgcgctgttg tttttgacgg tgacgatgtt cagcgtcagc ttcccgtggt tgtcgatgcc
      661 gcctccgtag ttggccgcgg agttcccgtc gagcgtacaa cggtagagcg tcaggctgta
      721 ccctctgttg aggatggcgc cgccgctctc cgcagcgttg cctcccctga tgatcaggta
      781 ttcgaaccgg gggccgaaga cgcccggcgc gatcgagaag actcgcttgc ccggggcggt
      841 ctgccggatt gtcgtcgggc tggctgttcc cgagcccaca atagtgagcg tcttggagat
      901 ggagagttcc tccacgaggt cgatcgtcat ccccgtcgat tcgaacgtga tgaagtcgtt
      961 gttccacgca ttgttcacgg cgtgccgcag cgacccctcc gccgggttcg acggattgtc
     1021 gtccgtcctc gtcaccttcc atatcgtcgc ttccgcccgc ggggcaaatc ccgtcagaca
     1081 gagcgcgatc gccgccgcta tcgcaagaca gcaggctttc atccttttca tcaagttcga
     1141 cctccttttg ctgtttaggc agtatcatca acggcggagg agattctagc gcggctgccc
     1201 ccgcagtctg cggatcattt gtctggaaac gcctctccct tcccctgaat gaggcgcgca
     1261 agcgcctcaa gacagaccgt aagaaaatgt acaaagtaaa agccggagag aggcgctcct
     1321 tgctccggcc ttcggaagag gctacggaat gatcttcccg gtatccatac cctcgccgcc
     1381 ggagattccg tccagctcgg tatcgtccag ttcgccgacc ttctgtttct ttttgagttc
     1441 ctccggcttc agatcatgtt ccagcttggc tccgaccttc ttctgctcct gcgtcatcgc
     1501 ggatctcctc cttattgtaa gcggcgcttg ccgccggtct tcccggattc gttcttcgcg
     1561 gggtcctcgc gaagaacgaa cgagcgtact ctcaatgggc cctatcataa accttcacac
     1621 tgtgtaagag tcaagaagca aacaaaaatt ctgatgatac tttccagccc cgtgtgtctt
     1681 cagtttcccc gctttcttcg cggaaggagc agcagcggcg ccccgaacag aagcgccaag
     1741 ggagacgctg cgctcgtcga gcagcccgat ccaccggacg ccgccgcggc cgtccgcgcc
     1801 tcggcacgga tcgaagccgc gcggaaggtc accgctccga tctgccggtt gtggtcgaac
     1861 ggtccgttgt cctccacgac gttgcgccag
//
LOCUS       DAOSWW010000566         1889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51454_length_1889_cov_3.616685, whole genome shotgun sequence.
ACCESSION   DAOSWW010000566 DAOSWW010000000
VERSION     DAOSWW010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51454_length_1889_cov_3.616685"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PK442_10505"
     CDS             <1..785
                     /locus_tag="PK442_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO88125.1"
                     /translation="DFVYHWSNTKSMFGIEQTKGEISRQINEHRDLRAVDEILAARDL
                     PDRDMGLWGFLSLVGQLGFQKRWAQTPRIPQTSVLGHLLFVAILSYFVSMEIGACPRR
                     RYNNFFGGLLHDLPEVLTRDIISPVKNSVAGLDELIKQLEKQAMEERILPLLPEAWRE
                     EIRYFTENEFAGKIRPHGSPSPVILQHDLGEEQNLDSLDPIDGRVIEACDKLSAYMEA
                     SLSIRLGVAPQALAEGKRNIYSRFGRSVISGFPMGQLFDYFW"
     gene            complement(773..>1889)
                     /locus_tag="PK442_10510"
     CDS             complement(773..>1889)
                     /locus_tag="PK442_10510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOO88126.1"
                     /translation="ACPELDFALEGTVAIVEERLAALGIPWRRVAGTGVAALMKGKPG
                     AGAVLLRADMDALPLREEVDVPWRSKNEGAMHACGHDVHTASLLGAAALLHKMGKQLP
                     CDVLLVFQPAEETSGGALPMLSDGLLEGTDVRAAFALHCDPSLPAGKVGVHGGAFRAA
                     SDMFDCVVRGRGTHGAEPHRGDDVIAIACRTVDALHQLVGRAVDPAEPAVISVGSFHA
                     GTARNILPERAEFSGILRTIDPETRVSLRAKIRRTVMNIPDVLGAVGEVRFTEGYPAL
                     INAPGMAGLLEGAVSDLLGPDALVRLTKPSMGVDDFSYFAERLPGCYFMLGTGHGNGR
                     EEAPLHSPRFAPDERAIALGAALLAECALRAGEGHQK"
BASE COUNT          355 a          656 c          562 g          316 t
ORIGIN      
        1 tcgacttcgt ttaccactgg agcaacacga aatcaatgtt cggcatagaa cagacgaagg
       61 gggaaatctc caggcagatc aacgaacacc gcgacctccg ggctgtggac gagatccttg
      121 ccgcgcgcga cctgcccgac cgcgacatgg ggctctgggg cttcctctcg ctggtggggc
      181 agctgggctt tcagaagcga tgggcgcaaa ccccaaggat tccgcagacg tcggtgctcg
      241 gacaccttct tttcgtcgcg atcctctcct acttcgtctc catggagatc ggcgcgtgcc
      301 ccagaaggcg gtacaacaac ttcttcggcg gccttctcca cgaccttccg gaggtgctca
      361 cgcgcgacat catttctcct gtgaagaact ccgtggccgg actcgacgag ctcatcaagc
      421 agctggagaa acaggctatg gaagagcgga ttcttccgct cctcccggaa gcgtggcgcg
      481 aagagatacg ttatttcacc gaaaacgagt tcgccgggaa gatacgtccg cacggctcgc
      541 cgtcgccggt gattctgcag cacgacctcg gagaggagca gaacctcgac agcctcgatc
      601 ctatcgacgg tcgcgtcatc gaggcgtgcg acaagctctc cgcctatatg gaggcctcgc
      661 tctcgatccg cctgggcgtc gctccgcagg cactcgcgga ggggaagcgg aacatctact
      721 cccgcttcgg ccgctcggtg atctccggtt tcccgatggg gcagctcttc gactacttct
      781 ggtgaccttc cccggcgcgg agggcgcact cggcgagaag ggcggccccg agagcaatcg
      841 ccctttcgtc gggggcgaag cgggggctgt ggagcggcgc ttcttcgcgt ccgttgccgt
      901 gccccgtgcc cagcataaaa tagcagccgg gcagtcgctc ggcgaagtag gagaagtcgt
      961 ccacccccat gctcggcttc gtcagacgaa cgagagcgtc cgggcccagg aggtccgata
     1021 ccgcgccctc cagcaggccg gccatcccgg gagcgttaat gagcgccgga tacccctcgg
     1081 tgaagcgcac ctcccccacc gcccccaaaa cgtccggaat attcatcacc gttctgcgga
     1141 tcttcgcccg taaactcacc cgcgtctcgg gatcaatcgt ccgcaggatc ccagagaact
     1201 ccgcccgctc gggaaggata ttccgcgccg tccccgcatg aaacgaaccg acggagatca
     1261 cggcgggctc ggcggggtcg acggccctgc cgacgagctg atgcagcgcg tcgaccgtcc
     1321 tgcacgcgat cgcgataaca tcatcccccc ggtgaggttc cgcgccgtgc gttcccctgc
     1381 cgcgtaccac gcagtcgaac atgtcggagg ctgcgcggaa agcgcctccg tgcaccccga
     1441 cctttcctgc gggaagcgac gggtcgcagt gcagcgcaaa cgccgccctg acgtccgttc
     1501 cctcaagaag gccgtcggaa agcatcggca gcgcgccccc ggacgtctcc tcggcaggct
     1561 gaaaaaccag cagcacatcg cagggaagct gtttccccat cttgtgaagc agtgcagcgg
     1621 cgcccaaaag actcgccgtg tgcacatcgt gcccgcacgc gtgcatggcg ccctcgtttt
     1681 tcgaacgcca cgggacgtcc acttcctccc gcagaggcag cgcgtccata tccgcccgca
     1741 acagcacggc gccggcgccc ggcttgcctt tcatcaacgc cgcgacgccg gtaccggcaa
     1801 cgcgcctcca tggaatcccg agagcggcga gccgttcttc cacgatcgca accgtgcctt
     1861 ccagcgcaaa atcaagctcg ggacacgca
//
LOCUS       DAOSWW010000567         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51817_length_1881_cov_2.810515, whole genome shotgun sequence.
ACCESSION   DAOSWW010000567 DAOSWW010000000
VERSION     DAOSWW010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51817_length_1881_cov_2.810515"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PK442_10515"
     CDS             <1..863
                     /locus_tag="PK442_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HOO88127.1"
                     /translation="FCQGFYALAERVKERSGGKMTVKVFPSAQLGSDEDVIEQAIQGV
                     NVAVVTDAGRMANYVKEIGIVGMAYFTNSYDELLKVQQTDFWKGNVEKLASENGIRVL
                     SFNWFDGARHFLTNKPVKTPEDLKGVRIRTPGAPSWSRSVAALGATPVTMGWNDVYNA
                     VQQKAIDGAEAQHSASYGLRVYEVLKYIDKTAHFQLLNGMIVGEKWFKTLPASYQALL
                     IEEFEKQGKLTAAEVIEKSDVFEKMMVEKGMEVVEVDLEAFKKASDAAYDELGFAELR
                     KKIYAEIGKE"
     gene            980..1489
                     /locus_tag="PK442_10520"
     CDS             980..1489
                     /locus_tag="PK442_10520"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOO88128.1"
                     /translation="MLDLVKKSYTFICRAEEFLTSFALCAITFLVFVSAIGRFVGYPI
                     NWAIDISLLLFAWGAFLGADIGIRKDRVINVDFLTSKMPLPVQKKIAVAWSVAIILFL
                     VVLIAYGIPLCISNAKRQFQNISLSYSYVTASLPVCAFLMIVSTAIKLKKQITDFPST
                     LRGGGADVV"
     gene            1509..>1881
                     /locus_tag="PK442_10525"
     CDS             1509..>1881
                     /locus_tag="PK442_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88129.1"
                     /translation="MLLLVGVFLALLFLGMPVAFAIGLSGFVFMLATPNIPLSIVVQR
                     IVSQTQSFTLLAIPLFIFAGNLMNATGITTRLVKLSRVLVGHMAGSLAQVSVIMSTLM
                     GGVSGSANADAVMECRILGPEM"
BASE COUNT          367 a          577 c          566 g          371 t
ORIGIN      
        1 ccttctgcca ggggttctac gcgctcgccg agcgtgtgaa ggagcgcagc ggcggaaaga
       61 tgaccgtcaa agtcttcccg agcgcgcagc tcggcagcga cgaagacgtc atcgagcagg
      121 ccattcaggg agtcaacgtg gccgtggtca ccgacgcagg ccgtatggcg aactacgtca
      181 aggagatcgg catcgtcggc atggcctact tcaccaacag ctacgacgaa cttctgaagg
      241 tccagcagac ggacttctgg aaggggaacg tcgagaagct tgcttctgag aacggaattc
      301 gcgtgctctc cttcaactgg ttcgacggcg cgcggcactt cctcacgaac aagcccgtga
      361 agacccccga ggatctgaag ggcgtccgta tccgcacccc gggcgcgccc tcctggtctc
      421 gttcggtcgc cgcgctcggc gccactcccg tcaccatggg gtggaacgac gtctacaacg
      481 cggtgcagca gaaggccatc gacggcgccg aagcgcagca ttcggcgagc tacggcctcc
      541 gcgtgtacga agtgctcaag tacatcgaca agacggcgca cttccagctc ctcaacggta
      601 tgatcgtcgg cgaaaagtgg ttcaagacgc ttccggcgtc gtaccaggcg ctgctgatcg
      661 aggagttcga gaagcaggga aagctcaccg cggccgaagt tatcgagaag agcgacgtct
      721 ttgagaagat gatggtggag aagggcatgg aagtcgtcga agtcgacctg gaagccttca
      781 aaaaagcctc cgacgcggcg tacgacgagc ttggcttcgc ggaactgcgt aaaaagatct
      841 acgcggagat aggcaaggaa taagccgtcc tccgaacgcc ccgttccgca cgggcggctt
      901 ttcggcaacc gtcgcgtttc cggatgcggc ggttgccttc tacttcgggc ccgccatcga
      961 aaggagggtg cgcaagaaca tgctcgatct cgtgaaaaag agctacacat tcatctgcag
     1021 agcagaggag ttcctgacct ctttcgctct gtgcgcgatt acatttttgg tcttcgtctc
     1081 cgccatcggc aggtttgtcg ggtatccgat caactgggcg atcgatattt ccctcctcct
     1141 cttcgcatgg ggagcgtttc tgggcgcgga catcggcatc aggaaggacc gcgtgatcaa
     1201 cgtcgacttc ctcacatcga agatgcccct tcccgtgcag aaaaaaatcg ccgtcgcctg
     1261 gtcggtcgcc atcatactct tcctcgtcgt gctgatcgcc tacggcattc cgctctgcat
     1321 ctcgaacgcg aagcggcagt tccagaacat ctcgctcagc tactcgtacg tcacggcgtc
     1381 gctgcctgtg tgcgcgttcc tgatgatcgt ctcgacggcg atcaaactga agaaacagat
     1441 cacggacttc ccgtccacgc tgcgcggcgg cggggcggac gtcgtgtagc gcgaagggag
     1501 gaagtccgat gctgcttttg gtaggcgtct ttctggcgct gctctttctg ggtatgccgg
     1561 tggccttcgc catcggtctg tcagggttcg tcttcatgct ggcgaccccc aacatacccc
     1621 tctccatcgt cgtccagcgc atcgtctcgc agacgcagag ctttacgctg ctggccattc
     1681 cgctcttcat cttcgcgggc aacctgatga acgccaccgg catcacgacg cgcctggtca
     1741 agctctcccg cgtactcgtg ggacacatgg cgggcagcct cgcgcaggtc agcgtcatca
     1801 tgagcacgct gatgggcggc gtctccggct cggcgaacgc cgacgcggtg atggagtgcc
     1861 gcatcctggg ccccgagatg a
//
LOCUS       DAOSWW010000568         1879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51895_length_1879_cov_3.662281, whole genome shotgun sequence.
ACCESSION   DAOSWW010000568 DAOSWW010000000
VERSION     DAOSWW010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51895_length_1879_cov_3.662281"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1084
                     /locus_tag="PK442_10530"
     CDS             149..1084
                     /locus_tag="PK442_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOO88130.1"
                     /translation="MLFGKEPTGFIFDVKRYSIHDGPGIRTTFFFKGCPLSCWWCHNP
                     EGISPLPAPTYHGNRCIACGRCVRECPNGARSFGPEGIALDSSKCVECRICEQVCPTR
                     ALEFVGKEMTVEEIVRLAKKDLAFYDESGGGVTFSGGEPLMQAEFLFACLEALRSLEI
                     STAVDTSGFCREEDLLLAAEEADIFLFDIKLIDPKLHEYYTGVGNERILANLRSLDKK
                     LVEQGRGSINIRIPLIPGVNNSPENLEAVIACVLSLRSVSGVNILPYHSIGSGKYKNL
                     GLEFKMGQTQPPSDYEVSQALSLFASRGIHAVKGG"
     gene            1090..>1879
                     /locus_tag="PK442_10535"
     CDS             1090..>1879
                     /locus_tag="PK442_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOO88131.1"
                     /translation="MNERIARLRNESFETYPSISIERARLTTDFYRANEGKYPVPVMR
                     ALNFKNLCEQKTLYIGKDELIVGERGPAPKAVSTFPELNCHTARDLEILNTRPMTGYR
                     VHEDDIRIYTEEVVPYWTGRTLRDRIFSNLPEEWLALYEAGCFTEFMEQRAPGHTALD
                     GTIYSTGLLDMKKKIASARERLDWINDPHATEKDEELQAMDIACDAAIIFARRHADLA
                     EEMAASEKDFVRKAELLKIADVCRHVPANAPRDFHEAIQMYWFTH"
BASE COUNT          476 a          469 c          499 g          435 t
ORIGIN      
        1 gtccttgatg agtgcggcta taccttcaga atgatcgcag tgtggatcga cggtactcct
       61 accgcagaga acatttcgcc accgccgtcg ttcctgacag agcggaagta caggctcttc
      121 atatgatcag cggaggttag acactgtaat gctcttcgga aaagaaccga caggatttat
      181 cttcgatgtg aaacggtact ctatccatga tggaccgggg atccgcacga catttttctt
      241 taaaggatgc cctctttcct gttggtggtg ccataatccg gaaggcattt cacctcttcc
      301 ggcgcccacg tatcatggaa acaggtgtat agcctgcgga cgatgcgttc gtgaatgtcc
      361 caatggagcc cgaagcttcg gccccgaagg aattgcgctg gactcttcga agtgtgtcga
      421 gtgcaggata tgcgagcaag tttgtcctac gcgtgctctc gagttcgtcg gcaaagaaat
      481 gactgtggaa gaaattgttc gcctcgcgaa aaaggatctt gctttttatg acgaatcagg
      541 cggaggtgtc actttctccg gaggagagcc tttgatgcag gccgagtttc tcttcgcgtg
      601 tctggaagcg ctccgctctc tggaaatttc tacggcagtg gatacttccg gcttctgtcg
      661 cgaggaggat cttcttctcg ccgcggaaga ggcggatatt tttcttttcg acatcaagct
      721 gattgacccg aagctgcatg aatactatac tggtgtaggt aatgaaagaa ttttagccaa
      781 tctgaggagc ctcgacaaaa agctcgtgga acagggaaga ggaagcatca atatcaggat
      841 tcccctcatt cccggtgtga acaattctcc ggaaaatctg gaggccgtga ttgcatgcgt
      901 tctttctcta cgaagcgttt cgggcgtgaa tattttgcca tatcactcta tcggatccgg
      961 caagtacaaa aaccttggat tggagttcaa aatggggcag acgcagcccc cgtcggatta
     1021 tgaagtttca caggcgcttt cactgtttgc atcgcggggg atccacgcgg tgaaaggagg
     1081 ataagagaaa tgaacgagag aattgcacgt ctgagaaatg agagtttcga aacatatccg
     1141 agtatttcaa tcgaacgagc ccgtttgacc accgatttct acagggccaa tgaaggcaaa
     1201 tacccggtgc ccgtcatgag ggcgcttaac ttcaaaaatc tttgtgaaca aaagacgctc
     1261 tacataggaa aagatgagct gatcgtgggg gagcggggcc ctgctccaaa ggcagtctcc
     1321 accttcccgg aactgaattg ccatacggca agagacctcg aaatcctgaa cacgcgtcct
     1381 atgaccggat acagggttca cgaagatgat attcgcatct acacggaaga agtcgtgccc
     1441 tactggaccg gccgcactct acgggacagg attttttcaa acctccccga agaatggctt
     1501 gcgctctatg aagcggggtg tttcaccgaa ttcatggagc aacgagcacc gggacacaca
     1561 gcgctcgacg gaacgatcta ttcaaccgga ctgctcgaca tgaagaaaaa aatcgcctcc
     1621 gctcgcgaaa ggcttgattg gatcaacgat cctcacgcca ctgaaaaaga cgaggagcta
     1681 caggcgatgg atattgcctg cgacgccgca attatcttcg cacgacgtca tgccgatctc
     1741 gccgaggaga tggcagcatc cgagaaggac ttcgtccgaa aggcagaact cctcaagatc
     1801 gccgacgtct gccgtcatgt tccggcgaac gctccgcgcg attttcacga ggctatccag
     1861 atgtactggt ttacacatc
//
LOCUS       DAOSWW010000569         1879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51911_length_1879_cov_2.813048, whole genome shotgun sequence.
ACCESSION   DAOSWW010000569 DAOSWW010000000
VERSION     DAOSWW010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51911_length_1879_cov_2.813048"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK442_10540"
     CDS             complement(<1..556)
                     /locus_tag="PK442_10540"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301715.1"
                     /note="Catalyzes the deamination of cytosine to uracil and
                     ammonia; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HOO88132.1"
                     /translation="MDLVVRNARLRGESGTKDIAVRDGLVAAIGERLAVQAGREIDAG
                     GNLASAPFIDPHLHLDAALSVGDPRYNESGTLLEGIAIWGERRPSLQKNEVLRNAREA
                     VLWEVANGVQFIRTHADTTDSALVTVEALVELREEMKDVAEIQVVAFPQDGVFTFPEG
                     EANLRRALDMGCDCVGAIPHIEFTR"
     gene            890..>1879
                     /locus_tag="PK442_10545"
     CDS             890..>1879
                     /locus_tag="PK442_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOO88133.1"
                     /translation="MKKFVALLALCLLVLFAGTAAADVKEYKDFKLAAVFQTAIEEPW
                     DGAIHQACLKVQKETGITYEFAEKVGAADFERVLREYAERGFDLIVGDAFLAGEEPSR
                     RVAKEYPEVAFAFGSEFTFQEPNYSVFDNWIHEPSYLCGVIAGRMTKSNTLGIVAAIP
                     IAEVNRLVHAFKEGALSVNPAVKVKVAYIGSWFDPPKAKEAALAQIESGVDIIFAERF
                     GVFEAAKEHKLLAFGNMLDQHDLAPEVVVTGPVWDMYPTVLHSVDAVRKGEWKAENLR
                     EWSMMAKGGAFLAPFHEFEQKLPAELIKEVRDLEAKILAGEFTVPVIETELKTD"
BASE COUNT          367 a          567 c          594 g          351 t
ORIGIN      
        1 cgcgggtgaa ttcgatgtgc gggatggcgc cgacgcagtc gcaccccata tcgagagcgc
       61 ggcgaaggtt cgcctcacct tccgggaagg tgaagacgcc gtcctgcgga aaggcgacca
      121 cctggatctc cgcgacgtcc ttcatctcct cgcgcagctc gacgagcgcc tccaccgtaa
      181 cgagggcgga atcggtggtg tccgcgtggg ttctgatgaa ctgaacgccg ttggcgacct
      241 cccagagcac ggcctcgcgc gcgttgcgga gcacttcatt cttttgaaga gaggggcggc
      301 gctcccccca gatggcgatg ccttcgagga gcgttcccga ttcgttgtaa cggggatctc
      361 ccacgctcaa ggcggcgtcg aggtgcagat gagggtcgat gaagggcgcc gacgccaggt
      421 tgcccccggc gtcgatctca cggccggcct gaacggcgag ccgttctccg atcgcggcca
      481 ccaggccgtc ccgaaccgct atatccttcg ttcctgactc tccgcgaagc cgcgcattgc
      541 gtaccacaag gtccattctc tcttctcccc gccgatttga tttatgcaat cgtttgcatt
      601 aaaatagtac cacaatcgct cttccctgtc cactgccgac aggaagagcg aatattcacg
      661 tcacccgtcg ccattgacag aagaacctct cgttgattga atgattgaaa atcatcgaga
      721 gtgcagaact tctgataaaa tgggaattgg cgcgattagg aaaaaccgcg gaggtcaagg
      781 aggtgacggg gcggaactct tccatgcgga acgtgggaat tcatggtctg tacgacggcg
      841 tcgggcatcc gcccgtctgc cggatactct gaacgggagg tgcatttgtg tgaagaaatt
      901 tgtagctctg cttgctcttt gtctgctggt tctcttcgcc ggaacggcgg cggccgatgt
      961 gaaggagtac aaggacttca agctcgcggc cgtcttccag acggcgatcg aggagccgtg
     1021 ggacggcgcg atccatcagg cgtgcctgaa ggtgcagaaa gagaccggca tcacctatga
     1081 gttcgcggag aaggtcggcg cggcggactt cgagcgcgtg ctccgcgagt acgccgagag
     1141 agggttcgac ctgatcgtcg gcgacgcatt cctcgcgggc gaggagcctt cgcggcgcgt
     1201 ggcgaaggag taccccgagg tcgcgttcgc cttcggctcg gagtttacct tccaggagcc
     1261 gaactactct gtcttcgaca actggatcca cgagccctcc tacctgtgcg gggtgatcgc
     1321 cggacggatg acgaagagca atacgctggg catcgtcgcg gccatcccaa tcgccgaggt
     1381 caacaggctg gtacacgcat tcaaagaggg cgcgctctcg gtgaacccgg ccgtcaaggt
     1441 gaaggtcgcc tacatcggaa gctggttcga tcctccgaaa gcgaaggaag ccgccctggc
     1501 ccagatcgag tcgggcgtcg acatcatttt cgctgagcgc ttcggcgtct tcgaggcggc
     1561 gaaggagcac aagctgctcg ccttcggcaa catgctcgac cagcacgacc tggcccccga
     1621 agtcgtcgtg acgggccccg tatgggacat gtacccgacg gtgctccact cggtcgacgc
     1681 ggtccgcaag ggcgaatgga aggccgagaa cctccgcgag tggtcgatga tggccaaggg
     1741 cggcgcgttc ctcgccccct tccacgagtt cgagcagaag cttcccgccg agctgatcaa
     1801 ggaagtccgc gacctcgagg cgaagatcct cgccggcgag ttcaccgtcc cggtcatcga
     1861 gacggaactg aagacggac
//
LOCUS       DAOSWW010000570         1878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_51943_length_1878_cov_4.166758, whole genome shotgun sequence.
ACCESSION   DAOSWW010000570 DAOSWW010000000
VERSION     DAOSWW010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51943_length_1878_cov_4.166758"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /gene="grdB"
                     /locus_tag="PK442_10550"
     CDS             complement(<1..1155)
                     /gene="grdB"
                     /locus_tag="PK442_10550"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(106..108),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HOO88134.1"
                     /translation="MVTYRIIHYINQFYAGIGGEEKADITPEIREGIVGPGQALKAAL
                     GAEAEIVATVICGDSYFASNMEKASEEILGMMSKYKPDAVVAGPAFNAGRYGTACGAL
                     GEAVVKKLGIPVVSGMYPENPGVEMYKKSFYIVETPDSAAGMRKAVPAMAKLVLKLLK
                     GEKLGTPSEEGYIERGIRKNKFYEKLAAERAVDMFTAKLKGEPFVTEYPMPVFDRVAP
                     NPAIKNMKDAVIALVTSGGICPKGNPDHIEASSASKFGEYDISGVMDLTCDSYCTAHG
                     GYDATYADQDADRVLPVDVLRDMEKEGVFKKLHNKFYTTVGNGTAVASAKRFAEEIAK
                     RLIADGVTAVILTSTUGTCTRCGATMVKEIERAGLPVVHICTIVPISLTVG"
     gene            complement(1170..1646)
                     /gene="grdA"
                     /locus_tag="PK442_10555"
     CDS             complement(1170..1646)
                     /gene="grdA"
                     /locus_tag="PK442_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_133955713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1515..1517),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HOO88135.1"
                     /translation="MGKLAGKKVLLLGERDGVPGPAMETCLKNSGADIVFSVTECFVU
                     TAAGAMDLQNQQRIKDAVEKYGPENVVVILGSSDAEGAEIYAETVSNGDPTYAGPLAG
                     VQSGLAVFHVFEQEIRDECDANAWEEQISMMEMVLDPEKLSEAVSGIRGQFSKYTL"
     gene            complement(1703..>1878)
                     /locus_tag="PK442_10560"
     CDS             complement(1703..>1878)
                     /locus_tag="PK442_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOO88136.1"
                     /translation="IVLPPMEKVIGVPEEANVIAGGWQGSLAADGTITVELQAILGST
                     SELGFTKLGAYTI"
BASE COUNT          370 a          561 c          495 g          452 t
ORIGIN      
        1 tcctaccgtg agggagatgg gaactatggt gcagatgtgc accacaggaa gacctgctct
       61 ttcgatctct ttgaccatcg ttgcaccgca acgagtgcag gttcctcagg tggatgtgag
      121 gataacggcg gtcactccgt ccgcgatcag ccttttcgcg atttcttcgg cgaagcgttt
      181 ggcactggcc acggcagtac cgttgccgac cgttgtatag aacttgttat gcagcttctt
      241 gaagacgccc tctttttcca tatcgcgaag aacgtctacc ggaagcactc tgtccgcatc
      301 ctgatcggcg tacgttgcgt cataaccgcc gtgggctgtg caatacgaat cgcatgtaag
      361 atccatcacg cccgagatgt cgtattcgcc gaatttgctc gcgctggagg cctcgatatg
      421 atcggggttc cctttggggc agataccgcc ggaagtgaca agcgcgatga ccgcgtcttt
      481 catatttttg atcgcgggat tgggggccac gcggtcgaag acgggcatcg gatattcggt
      541 gacaaacggc tctcccttga gctttgcggt gaacatatca accgcgcgct ctgcagccag
      601 cttctcgtag aacttgttct ttctgatgcc gcgctcgatg tatccttcct cggaaggcgt
      661 accaagcttc tcgcccttaa gcagcttcag gacaagcttt gccatcgcgg gaacagcctt
      721 ccgcatgccg gcggcgctgt cgggagtctc cacgatgtaa aaactcttct tgtacatctc
      781 gacgcccggg ttctccgggt acataccgga gacgaccggg ataccaagct tcttcactac
      841 ggcttcgccg agagcgccgc acgcagtgcc gtaccgtccg gcgttgaatg caggaccggc
      901 gacaaccgcg tcgggtttgt acttgctcat catcccaaga atttcctcgc tcgccttctc
      961 catattcgag gcgaaatacg agtcgccgca gatgacggta gccacgattt cggcttcggc
     1021 cccaagagct gcctttaaag cctgtccggg gccgactata ccttcgcgaa tctccggcgt
     1081 tatgtcggcc ttctcttcac cgccgattcc ggcgtaaaac tgatttatat agtgtatgat
     1141 ccggtaagtc acgatgacga cctcccctcc tacagggtgt atttactgaa ctgcccgcga
     1201 atgccgctga cggcctcgga cagtttctcg ggatcaagca ccatttccat catgctgatc
     1261 tgctcttccc aggcgtttgc atcgcactcg tcccgaatct cctgttcaaa aacatgaaat
     1321 acagcgagtc cagactggac tccggccaag ggacctgcat acgtcggatc tccgttgctc
     1381 accgtttcgg cgtagatttc tgcgccttcg gcatcggacg atccgagaat gacgaccacg
     1441 ttttcagggc cgtacttttc aacagcgtcc ttgatgcgct gctgattctg cagatccatg
     1501 gctcctgcgg cggttcagac gaagcattcg gttaccgaga agacgatgtc ggcgccgctg
     1561 ttcttcagac acgtttccat agcggggcct ggaacgccgt cgcgttcacc gaggagaagc
     1621 acttttttgc ctgccaattt ccccatgctc ttgcacctcc ttgtcgaaaa acttgtatcc
     1681 gggtattccc ggtttggtca catcagatgg tgtacgcgcc cagcttcgtg aaaccaagct
     1741 cgctcgtgga acccagtatt gcctgaagct cgacggtaat cgtcccgtcg gcggcgagag
     1801 aaccctgcca gcctccggcg atgacgttgg cctcttcggg aacgccaatg accttctcca
     1861 taggaggcag cacaatca
//
LOCUS       DAOSWW010000571         1871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_52263_length_1871_cov_3.351872, whole genome shotgun sequence.
ACCESSION   DAOSWW010000571 DAOSWW010000000
VERSION     DAOSWW010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52263_length_1871_cov_3.351872"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="PK442_10565"
     CDS             <1..1529
                     /locus_tag="PK442_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOO88137.1"
                     /translation="QGEQAYAGGIVGYAGSGVINWGTMHLSYCTMTGNRTYWQGAGVN
                     NTKTLTMTNCTVKGNFSNTGGGGVYSRGTLVIQDSVVEDNLCYDDFGGGIANEGTLTV
                     KRCVVRNNTANSPLSQSDIILGTNPGYGGGIGSLSGAVAIYDSSIRGNQGHFGGGIYG
                     KNGSITLQDSSVTGNTAGAFGGGIYCFKGSVTLQTSRITDNSLTADYYGGDLCLSSST
                     ASLLVETVISGNAPDQIWSPAPSSWTSDGTCTVGDAPNRSATAFSGYSGDAEPEPRSI
                     IGDADVAEVKTALANPESALYGAIEQALAADLGKSAAEKSAALVRLSGMAATLYYANT
                     FENVALTSTDLSVEYTASWPENVRYYSLFSKADGSGYENPGRGIQFEIKAGQSLPDGA
                     TPPDFYEEGEGLMTWRNVVTDGGSFDLEPAPGIVTFRVCSVRAAEATGDTGSGGCNAS
                     GGFAPLALLLLVPVQAFLFRRSCSGVPVPAAKSVVTAGMVSSAERKLVFQIRKGDSEE
                     "
     gene            1526..>1871
                     /locus_tag="PK442_10570"
     CDS             1526..>1871
                     /locus_tag="PK442_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88138.1"
                     /translation="MRQTKSHVFFMLLLLFALMPTFFPGASRAFGDTASNGAATVLEV
                     VDTSDDPSNPAPGTLRYLALHASDGDIIRFAEGKNDISLKATLEIDTSLKILGPATLT
                     QTGSGAVVVLKAQ"
BASE COUNT          360 a          563 c          613 g          335 t
ORIGIN      
        1 gccagggaga acaggcctat gccggcggta ttgtgggcta cgccggttcg ggcgtcatca
       61 actgggggac gatgcacctg tcgtactgca cgatgaccgg gaaccgaacg tactggcagg
      121 gagcgggcgt caacaatacg aagacgctca cgatgacgaa ctgcaccgtg aagggaaatt
      181 tctccaacac cggcggaggg ggcgtctact cccgagggac gctcgtcata caggactccg
      241 tcgtcgagga caacctctgt tacgacgact tcggcggcgg gatcgccaac gaggggacgc
      301 tgaccgtaaa acgttgtgtt gtgcggaaca acacggccaa ctcgcccttg tcgcaaagcg
      361 acattattct gggcacgaat cccgggtacg gcggcgggat cgggagcctg tccggggcgg
      421 tcgccatata cgactcttcg atcaggggca accaaggcca cttcggcggc gggatttacg
      481 gcaaaaacgg gagcatcacc ctccaagact cgtcggttac gggcaatacg gccggcgcgt
      541 tcggcggcgg catctactgc ttcaagggaa gcgtgacgct ccagacgtcg cggataacgg
      601 acaactccct gaccgcggat tactacggag gcgacctctg cctctcgtcg agcaccgcct
      661 cgctgctggt cgagacggtc atcagcggca acgccccgga tcagatctgg agtcccgctc
      721 cgagttcttg gacgagcgac ggcacgtgca cggtcggcga cgcgccgaac aggagcgcga
      781 cggcgttttc cggttattcg ggcgatgccg agcccgaacc tcgctcgatc atcggcgacg
      841 ccgacgttgc ggaagtgaaa accgccctcg cgaacccgga gagcgcgctg tacggcgcga
      901 tcgaacaggc gttggcagcg gatctgggga aaagcgccgc cgaaaagagc gctgcgctcg
      961 ttcggctctc cggcatggcg gcgacgctct attacgcgaa cacgttcgag aacgtcgccc
     1021 tgacgagtac ggatctttcc gtggagtata cggcctcgtg gccggagaac gtccgatact
     1081 attcactctt ctccaaggcc gacggctccg ggtacgagaa ccccggacgc ggcatccagt
     1141 tcgagatcaa ggcggggcag agccttcccg acggcgcgac gccgccggac ttctacgagg
     1201 agggcgaagg gctgatgacg tggaggaacg tggtgacgga cggcggctcg ttcgaccttg
     1261 aaccggcgcc cggaatcgtg accttccggg tctgctcggt ccgtgccgcg gaggctacgg
     1321 gcgacacagg cagcggaggt tgcaacgctt ccggaggctt cgcgccgctc gcgctgctgc
     1381 ttcttgttcc tgttcaggcg ttcctgttcc ggcgttcctg ttccggcgtt cctgttccgg
     1441 cggcgaagag tgtcgtaacc gcgggaatgg tttcatccgc ggaacgaaaa ctcgtttttc
     1501 aaatacggaa gggagatagc gaagaatgag acaaacgaaa tcccatgttt tttttatgct
     1561 gctgttgttg ttcgcactga tgccgacgtt ttttcccggg gcgtcccgcg cctttgggga
     1621 tacggcttcc aacggcgccg cgacggtcct ggaggttgtc gatacctccg acgacccgtc
     1681 gaatcccgcg ccggggacgc tccgctatct ggcgcttcat gcctcggacg gcgatatcat
     1741 ccgctttgcg gagggaaaga acgacatctc cctcaaggcg acgctcgaga tcgacacgag
     1801 cctcaagata ctgggccctg cgacgctgac gcagacggga agcggcgcgg tcgtggtcct
     1861 gaaggcacag g
//
LOCUS       DAOSWW010000572         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_52352_length_1869_cov_4.478501, whole genome shotgun sequence.
ACCESSION   DAOSWW010000572 DAOSWW010000000
VERSION     DAOSWW010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52352_length_1869_cov_4.478501"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PK442_10575"
     CDS             complement(<1..946)
                     /locus_tag="PK442_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88139.1"
                     /translation="MDIGYISVLLFGSMFLFLLLGLPVAFVLGGLATVFTAIFWGPES
                     LFIIVARTYSMMSTTTLVACPLFVLMATVLERSGVSEDLYDMMYRWMGSLKGGLAIGT
                     VIACTLIAAMSGIASTGVVMMGMMALPAMLKRGYDKRLATGCILAGGVLGPLIPPSIA
                     LVLYGTIAQVSIGALFAGGMSAGLLCSVLIIGYILIKCHLNPEMGPAIPEEERADWGA
                     KMKSLKGIFLPMLLVFSVLGSIYSGIATPTEAAAVGALGAIVCSALHGRLTWKLLRDA
                     SYATIKVQGFMMWILFAAQAFAAVYMGMGASRMVVGLVK"
     gene            complement(956..1486)
                     /locus_tag="PK442_10580"
     CDS             complement(956..1486)
                     /locus_tag="PK442_10580"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOO88140.1"
                     /translation="MIKFINFTDTLNDWVGKIFGFLVYPTMLVLVYEVVMRYGFNRPT
                     IWAHETSCMLYGAHFILGGAYALRWGGFVNVEAFYILFSPRVRAIIDLFTWTLFYAFV
                     GTLLWFSLPWAWESLQMMEYSNSTWGPYVWQLKLTIPAAAFLVLLQGMTKTIKDLHLA
                     LTGRELIPSAEAPVEN"
     gene            complement(1595..>1869)
                     /locus_tag="PK442_10585"
     CDS             complement(1595..>1869)
                     /locus_tag="PK442_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88141.1"
                     /translation="KKIVEEATVEFNKTFAKVCDEQEKEMFAKSFKEWGTTYIEWGKD
                     DIDRITKEFSLPFLNKIEQEIGSKDPRVAEGIQIIKQFMKDRGYIN"
BASE COUNT          440 a          555 c          510 g          364 t
ORIGIN      
        1 ccttgacgag tccgacgacc atccgcgaag cgcccatacc catgtagacc gccgcgaatg
       61 cctgtgcggc gaacaagatc cacatcatga atccctgcac cttgatcgtt gcgtacgacg
      121 cgtctctgag gagcttccag gtcagtcttc cgtggagcgc gctgcacacg atggcgccga
      181 gagcgcctac tgcggcggcc tcggtcggcg tggcgatgcc gctgtagatc gaccctaaaa
      241 ccgagaagac cagcagcatg gggaggaaga tacccttgag ggacttcatc ttcgcccccc
      301 aatcggcgcg ttcctcttcc ggaatggcag gccccatttc agggttgagg tggcatttga
      361 tcaggatgta gccaatgatc agaacggagc agagcaaccc cgcgctcatg cccccggcga
      421 aaagagcgcc gatggagacc tgagcgattg ttccgtagag caccagggcg atgctcgggg
      481 ggatcagcgg tccaagaacg ccgcccgcga ggatgcagcc tgtggccagc cttttgtcgt
      541 acccgcgttt gagcatcgcg ggaagggcca tcatgcccat catgacaacg ccggtggagg
      601 cgatgcccga catggccgct atcaaggtgc acgctatcac cgtgccgata gcaagccccc
      661 ccttgagaga gcccatccag cggtacatca tatcgtacag atcttcagaa accccggagc
      721 gctccagcac ggtcgccatc aggacgaaaa gagggcaggc caccagcgtc gtggtcgaca
      781 tcatggagta cgttcttgcc acgatgataa agagggactc cgggccccag aaaattgcag
      841 tgaacaccgt tgccagtcct ccgaggacga acgcgacggg aagcccgagc agcaggaaga
      901 gaaacatgct gccgaagaga agaacggaaa tatatcctat gtccataata gcacctcagt
      961 tctcaaccgg agcttcggcc gagggaataa gttcgcggcc ggtcagcgcc aggtggagat
     1021 ccttgatggt cttggtcatc ccctggagca gcacaagaaa cgccgctgcg ggtatggtca
     1081 gcttgagctg ccagacgtac gggccccacg tgctgttcga gtactccatc atctgcaaac
     1141 tctcccacgc ccacggcaaa ctgaaccaga gcaaagttcc gacgaacgcg taaaaaagcg
     1201 tccacgtaaa gagatcgatg atagcgcgca cccgcggtga gaagagaatg tagaacgcct
     1261 ctacgttgac gaaccctccc cagcggaggg cgtacgctcc tccaagaata aaatgcgccc
     1321 cgtacagcat acacgaagtt tcgtgcgccc atatcgtagg gcgattgaat ccgtagcgca
     1381 taaccacctc gtacacgagt acgagcattg tcgggtacac gagaaagccg aaaatcttcc
     1441 ccacccagtc gttcagtgtg tcggtaaaat taataaactt gatcaaaaag cccaatcgcc
     1501 tcacctcgcc gattctgaaa aaattgaaag cgcgccccct cctccggaga gagaaggggg
     1561 gcgcttcgtg gatgcgtcgg gtggacgctc cctgttagtt gatgtacccg cgatccttca
     1621 tgaactgctt gatgatctga ataccttcgg caaccctcgg atccttcgag ccgatttcct
     1681 gttcgatctt gttcaggaag gggagagaga attctttcgt tatcctgtcg atgtcatcct
     1741 tgccccactc gatgtaggtc gtcccccact ccttgaagga cttcgcgaac atctcttttt
     1801 cctgctcgtc gcacactttg gcgaacgtct tgttgaattc tactgtagct tcctcgacga
     1861 tcttcttca
//
LOCUS       DAOSWW010000573         1864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_52576_length_1864_cov_4.004975, whole genome shotgun sequence.
ACCESSION   DAOSWW010000573 DAOSWW010000000
VERSION     DAOSWW010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52576_length_1864_cov_4.004975"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PK442_10590"
     CDS             <1..1358
                     /locus_tag="PK442_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO88142.1"
                     /translation="VPRHMEKGRQLVEILKVQYQSWRKIHGDIDRLVEEILRSQSVDR
                     KNTLFQELKAQVYRSIPISDVMGSTFDDLTRNNNEQTDLLVEGDLKRAGMMEMFSLLA
                     MIVGVFIALVMTVKISRSIVLPIASAVSFLSRLREGELRENVPQEFLMRKDEIGTLAQ
                     AVQDLTEELREQIASMKEVTVALASSAYQISASVSEVTAGAEETAVAVVETTATMEEV
                     RTTAEVTNRKSKEVAESAQQGLQVVQKGKSATDALFDGMKNVGERMTSIAETIIRLSE
                     QSQEVGEITETVEDLAEQSNLLAVNAAVEAAKAGEQGRGFSVVAQEIKSLAEQSKQSA
                     KEVQRILRDIQKATSAAVMAIEQGSKAVEQGAKDAAPSRESIQGITKKFVESAQSAAQ
                     IAAANNELLAGIDQVTQAMESIKAAGEQNVAGMKDLEAASGGLKEMGQKLSLLIERYT
                     V"
     gene            1384..>1864
                     /locus_tag="PK442_10595"
     CDS             1384..>1864
                     /locus_tag="PK442_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOO88143.1"
                     /translation="MNQAEREFLEELRRDFVPEAEEYLQTIVSGLLALEKSPASSSGV
                     IEEVFRAAHSLKGAAQAVQMPGVSSICQVVESVFSGMKKGVLHLSEGDFDILQRAADT
                     LSAMIGAPEKEDASGITPLIHEIESLLDPAGGAAPPKTPLLQGSRRPHAEEDEPEGKV
                     "
BASE COUNT          432 a          468 c          625 g          339 t
ORIGIN      
        1 cagtgccgcg acacatggag aaagggagac agcttgtaga aattctgaag gttcagtatc
       61 agtcctggcg taaaattcac ggggatatcg acaggcttgt ggaggagatc ctccgaagcc
      121 agtcggtcga cagaaagaat acgctttttc aggagttaaa ggcccaggta taccggtcga
      181 ttcctatctc ggatgtcatg ggctccactt ttgatgatct cacccggaac aacaacgaac
      241 agaccgatct tcttgttgag ggggatttga agcgcgcggg aatgatggag atgttttccc
      301 tccttgcgat gatcgtcggc gtattcatcg cgctcgttat gacggtgaag atatcccgga
      361 gtatcgtcct tccgatagct tccgcggtct ctttcctctc ccgccttcgc gaaggggaat
      421 tgcgtgaaaa cgtcccgcag gagttcctta tgaggaagga cgaaattggc acgctggcgc
      481 aagcagtcca ggatttgacg gaggagcttc gggagcagat cgcctccatg aaggaagtga
      541 cggttgccct ggcgtcttcc gcataccaga tttccgcctc ggtttccgag gtcaccgcag
      601 gcgccgagga aaccgcagtc gcggtcgtgg agaccacggc gaccatggaa gaggtgcgca
      661 ccaccgccga ggtgaccaac agaaagagca aggaagtcgc cgagagcgcg cagcaggggc
      721 tgcaggtggt gcagaagggg aaatcggcga ccgacgctct gttcgatggg atgaaaaacg
      781 tcggagagcg catgacctcg atagccgaga cgattatccg cctcagtgaa cagagccagg
      841 aggttgggga gatcaccgag acggtggagg accttgccga acagtccaat ctgcttgcgg
      901 tgaacgcggc cgtggaggcc gcaaaggccg gggagcaggg gcgggggttc tccgtcgtgg
      961 cgcaggaaat aaagagcctt gcagagcagt cgaagcagtc cgcgaaagag gtgcagcgca
     1021 tcctccgcga tatccagaag gccacgagcg ccgcggtgat ggccatcgag cagggatcga
     1081 aagccgtgga gcagggagcg aaggatgcgg cgccttccag ggagtcgatt caagggatca
     1141 cgaaaaaatt cgtcgagtcc gcgcagtctg ctgcgcagat cgccgcggcg aacaacgagc
     1201 tgctggctgg tatcgaccag gtgacgcagg cgatggagag catcaaggcc gcgggggagc
     1261 aaaacgtggc aggaatgaag gacctggaag cggcttcggg cggtctgaag gagatgggac
     1321 aaaaactctc gcttctgatc gaacggtata cggtgtgagg ccccgaggta cgaaggagcg
     1381 ctcatgaacc aggcggagag ggaatttctt gaggaactgc gcagagattt tgttccggag
     1441 gcggaggagt atctccagac catcgtctcc ggtctgcttg ctcttgaaaa gagtcccgca
     1501 agctcttccg gggtcatcga ggaggtcttc cgggctgccc atagcctcaa aggggcggcg
     1561 caggcggtac agatgccggg ggtctcttcg atatgccaag ttgtggagag cgtcttttca
     1621 ggcatgaaaa aaggggtcct tcatctctcc gaaggagatt tcgacatcct ccagcgtgct
     1681 gccgatactc tgtccgccat gataggcgct ccggagaagg aggacgcgag cggcataact
     1741 ccgctgattc atgagattga aagcctgttg gatccggcgg gcggcgccgc tccgccgaaa
     1801 acgcctttgt tgcaggggag ccggagaccg cacgcggagg aagatgagcc ggagggaaaa
     1861 gttg
//
LOCUS       DAOSWW010000574         1864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_52598_length_1864_cov_2.776672, whole genome shotgun sequence.
ACCESSION   DAOSWW010000574 DAOSWW010000000
VERSION     DAOSWW010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52598_length_1864_cov_2.776672"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PK442_10600"
     CDS             <1..574
                     /locus_tag="PK442_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOO88144.1"
                     /translation="ASLVGSPQIRNLGTIGGNLANASPAADSLPPLCALEASLLIENA
                     EGGKSTISPAQIASAPGKNSLDADQYIRAVRLPLRENLLSFFVKTGSRKAVAISKLSL
                     ALALSSEPGHVRLFAGSLGTSPIRMEAAEAVLDRRDWTEANHTAFLDALSDEVERAIP
                     GRASLPFKRLAVRGLGDDLWTAAKEAISRG"
     gene            567..>1864
                     /locus_tag="PK442_10605"
     CDS             567..>1864
                     /locus_tag="PK442_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO88145.1"
                     /translation="MAELKYVGRDVQRNDIVEKASGRMKYMGDRMQGGAAHARLLLSP
                     IARGRVVSFDASEAERMPGVLRVFSPKDDPGKRFNSALFLPDQTDLQDHPVFTDKPLF
                     VGDILGAVLAKDEETARAAAAAVKIGFEEEAPLLDMEQAALPQTPSFREGMPPVIEGK
                     IAYGEETLPGDAIILESVLRTQKIHHGAMENHICEARMEYGGVLLVETPCQMAFTVRY
                     VLADLFGIPFNKVRVVKTPVGGAFGGKQEVGIEPHCALMALKTGLPVRLALDRTQTIL
                     ATRTRAASVGRILIAADREGNLLRREMDVIVDAGAYATGTQRVMMAMGKKTSRLYRIP
                     SQSFRGRTVFTNTTPAGACRGYGSPQIHAITEIAMDLLARKLLLDPAEFRMKNLVHPW
                     DTDPAGGPALGNARIRDCLVRGMEVFRWNERRVIPSGTGR"
BASE COUNT          352 a          589 c          584 g          339 t
ORIGIN      
        1 ggcgtcgctc gtcggttccc ctcagatacg caaccttgga acgatcgggg gcaaccttgc
       61 caatgcctct ccggcagccg acagcctacc ccctctctgc gcgctcgaag cgtcgttgct
      121 catcgagaac gcggagggag ggaaaagtac gatttctccc gcacagatcg catcggcccc
      181 cggaaagaac tcgctcgatg cggaccagta tatccgcgcc gtccgccttc cgcttcggga
      241 gaacttactc tccttcttcg tcaagaccgg cagccggaag gccgtcgcta tctcgaaact
      301 cagccttgcc ctcgcgctct cctccgagcc ggggcatgtg cggcttttcg ccgggtctct
      361 ggggacttca ccgatccgca tggaagctgc ggaagccgta cttgaccggc gcgactggac
      421 tgaagcgaac cataccgcgt tcctcgacgc gctctcggac gaggtcgaac gcgcaattcc
      481 gggaagagcg tctctcccgt tcaaacgcct tgccgtacgc ggactgggag acgacctgtg
      541 gacggcggca aaggaggcta tttcccgtgg ctgagttgaa gtacgtcgga cgcgacgttc
      601 aaagaaacga tatcgtcgaa aaggcctcgg ggcggatgaa gtacatgggc gaccgtatgc
      661 agggaggagc ggcgcacgca cgcctgcttc tcagtccgat cgcgaggggg cgcgtcgtct
      721 ccttcgacgc ctccgaagcg gagcggatgc ctggcgtcct gcgcgtcttc tctccgaagg
      781 acgatccggg aaagcgcttc aacagcgcgc tctttctccc cgaccagacc gatttgcagg
      841 atcaccccgt ctttacggac aaaccgctct tcgtcggcga cattctcggc gcggtgctcg
      901 ccaaggacga ggaaaccgcc cgcgcagcgg ccgccgccgt caagatcggt tttgaagagg
      961 aggcgcctct gctcgatatg gagcaggccg cgctcccgca gacgccctcg tttcgggagg
     1021 gaatgccccc ggtgatcgaa ggcaaaatcg cctacggcga ggaaacgctt cccggggatg
     1081 cgatcattct cgaaagcgtc ctccggacgc agaaaattca ccacggcgcg atggaaaacc
     1141 acatctgcga ggcaaggatg gagtacgggg gcgtcctcct tgttgaaact ccgtgccaga
     1201 tggcgttcac tgtccgctac gtgctcgccg atcttttcgg catcccgttc aacaaagtgc
     1261 gcgtggtgaa aactccggtc ggcggagcat tcggcggcaa gcaggaagtc ggcatagaac
     1321 cgcactgcgc tctgatggcg ctgaagacgg gactccccgt tcggcttgct ctcgaccgta
     1381 cgcagaccat cctcgcgacg aggaccaggg ctgcctccgt cggacggatt ctcatcgccg
     1441 cggacagaga agggaacctt ctccggcgtg agatggacgt gatcgtggac gctggcgcct
     1501 atgccacggg gacgcagcgg gtaatgatgg cgatggggaa gaagacctcc cggctctacc
     1561 gtattccgtc ccagtcgttc cggggacgca ctgttttcac gaacaccacc cctgcgggcg
     1621 catgccgagg gtacggttcc ccgcagattc acgcgatcac ggagattgcg atggacctgc
     1681 tcgctcgaaa gctcttgttg gacccggcgg aattcaggat gaaaaacctt gtccatccgt
     1741 gggacacgga tccggcgggc gggcccgctc tcggaaatgc gcgcatccgc gactgcctcg
     1801 tccgcgggat ggaggttttc aggtggaatg agcggcgcgt gattccgtcc ggcacggggc
     1861 gctt
//
LOCUS       DAOSWW010000575         1864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_52601_length_1864_cov_2.698729, whole genome shotgun sequence.
ACCESSION   DAOSWW010000575 DAOSWW010000000
VERSION     DAOSWW010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52601_length_1864_cov_2.698729"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK442_10610"
     CDS             complement(<1..213)
                     /locus_tag="PK442_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88146.1"
                     /translation="MKAELTFTEEMLGTSSANADLHKEFIASKSADKEKMQEELESLP
                     ADELQQKAMTVFPRQDGSPFLWDYQVK"
     gene            complement(253..321)
                     /locus_tag="PK442_10615"
     ncRNA           complement(253..321)
                     /ncRNA_class="hammerhead_ribozyme"
                     /locus_tag="PK442_10615"
                     /product="hammerhead ribozyme type II"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02276"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02276"
     gene            complement(323..682)
                     /locus_tag="PK442_10620"
     CDS             complement(323..682)
                     /locus_tag="PK442_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88147.1"
                     /translation="MKFKVWIEGEKVPENADEEINNDEAYERIGEPLEAGTYDTEEEA
                     IARMEELHGSEESERETAPVPAAIARVLANLDALYANHPDNDDSTLTFEEYDDLKPEL
                     LEALENAVRSEWPPESD"
     gene            complement(710..1165)
                     /locus_tag="PK442_10625"
     CDS             complement(710..1165)
                     /locus_tag="PK442_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88148.1"
                     /translation="MTATTIKSRGMVDKRELVDTCGCVLQRTDAGLHMNDCSLHAAAK
                     RMYEVGQEFAEWSARAVERWEAGDLAEAVRNLDYARKEWRKVTAEIKEPPEETRAERI
                     EAAARRILMLDFYPHDGLLEVVKGEHKENCGEMECEVCSALRQLKQALA"
     gene            complement(1237..>1864)
                     /locus_tag="PK442_10630"
     CDS             complement(1237..>1864)
                     /locus_tag="PK442_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88149.1"
                     /translation="DNDPVIVVVSTDSNEPSEWSAAAFIATVRIAQQFRPVHVWWQGA
                     WLDDHGRERGYVFHAPLVTNDMDYSRLDYVLADDTRDNLSFGVLYSIAKIGDKVGRLQ
                     GMGRRADRAYMSGAKFVDHNGIRPKPEQIASHACRWLGMATPWAMEYATDQMAESALQ
                     EIPDRTPYVDTRTEREKARERREFREWDRRQERQTAERARERRSKAEI"
BASE COUNT          296 a          580 c          523 g          465 t
ORIGIN      
        1 cttgacctga taatcccaca ggaacggaga gccgtcctga cgcgggaaga cggtcattgc
       61 cttctgttgg agttcatccg caggaaggga ttcaagttcc tcctgcatct tttccttgtc
      121 ggcggatttg ctcgcgatga attccttgtg cagatccgcg ttggcgctcg acgtgccaag
      181 catttcctcc gtgaatgtga gttctgcctt catgcgcctg taccctcctg tgcttgcccc
      241 tgccagggcg tcgaggatct gcctcgtcag cgccgggaga tcatccccgg cggaccccgg
      301 aagcgttccg gggtttcggc ggtcaatcgc tttcgggtgg ccactccgaa cgcacggcgt
      361 tttcaagcgc ttcgagaagt tccggcttga ggtcgtcgta ttcctcgaag gtcagggtgc
      421 tatcatcgtt gtcgggatgg ttcgcataga gcgcgtcgag gttggccagt acccgggcaa
      481 tcgccgctgg aactggcgcg gtttcgcgct cgctctcttc gctaccgtga agctcttcca
      541 tccgggcgat tgcctcttcc tcggtgtcgt acgtgccagc ctccagcggc tcgcctatac
      601 gttcgtaagc ttcatcgttg tttatttcct cgtcggcgtt ctccggtacc ttttcgcctt
      661 cgatccatac cttgaacttc attgctcgtt ctcctttccg gcccggcgct caggcaagcg
      721 cctgtttgag ttgacgaagc gcagagcaca cttcgcattc catttctccg cagttctcct
      781 tgtgttctcc cttgaccact tccagcaatc cgtcgtgagg gtagaagtcg agcatcagaa
      841 tgcgccgcgc cgcagcttca atgcgttcgg ctcgcgtctc ttccgggggc tccttgattt
      901 ctgccgtcac cttgcgccat tctttccgtg cgtagtcgag gttgcgcacg gcttccgcaa
      961 ggtctccggc ttcccatcgc tctacggcac gggcggacca ctcggcaaac tcctgaccga
     1021 cctcgtacat gcgcttagcg gcggcatgca gagagcagtc attcatatgg agaccggcgt
     1081 ctgtacgctg gagcacacag ccgcaggtat cgaccagctc cctcttgtct accattcccc
     1141 ttgatttgat ggttgtggct gtcatggcgc tttgctctcc cgtttcgccc tgctggggct
     1201 cgtcagcggc gcgacaatag cgccggacgg aaaaacctat atctcggcct tgcttctccg
     1261 ttcacgggct ctctcggctg tctggcgctc ttggcggcgg tcccattcgc ggaactcgcg
     1321 gcgttccctt gccttctctc gttctgtgcg ggtgtcaacg taaggggtgc ggtctggaat
     1381 ctcctgtaag gcgctctcgg ccatctgatc ggttgcgtat tccatcgccc aaggggttgc
     1441 catcccgagc catcggcagg catgtgaggc tatctgttca ggcttcgggc ggattccgtt
     1501 atggtcaacg aacttcgcgc cagacatgta cgcacgatcg gcgcgccgtc ccatgccttg
     1561 aagcctgccg accttatccc cgatttttgc tatcgaatag agcacgccga aagagagatt
     1621 gtctcgggtg tcatcggcca gtacgtagtc aaggcgggaa tagtccatat cgttcgtgac
     1681 cagcggcgcg tggaaaacgt acccgcgttc gcggccatga tcgtcaagcc atgcgccttg
     1741 ccaccacaca tgcaccgggc ggaactgttg tgctatccgc accgtggcga tgaacgcggc
     1801 ggcggaccat tcgctaggct cgtttgaatc ggtcgagaca acaacaataa ccgggtcatt
     1861 atcc
//
LOCUS       DAOSWW010000576         1859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_52826_length_1859_cov_2.997783, whole genome shotgun sequence.
ACCESSION   DAOSWW010000576 DAOSWW010000000
VERSION     DAOSWW010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52826_length_1859_cov_2.997783"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PK442_10635"
     CDS             <1..402
                     /locus_tag="PK442_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE-related selenium metabolism membrane
                     protein"
                     /protein_id="HOO88150.1"
                     /translation="ASFMVKDAHLFNGVAAFVVVAFAVNYALGMFKPGFEGQPVAHTN
                     QMWNFLGMVLSGLAFTLAGGCPGRQVIMSGEGDGDSAIFVLGMLVGAGFAHNFSLASS
                     PAGVTAYGMTATAIGMVFCLAVGFLFRIKMD"
     gene            420..638
                     /locus_tag="PK442_10640"
     CDS             420..638
                     /locus_tag="PK442_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOO88151.1"
                     /translation="MADTVIVDARGLSCPQPVLETKKALEKLSGGRVEVLVDTVTSRE
                     NVARFGGSRGWKVEKEEREDGFCVVLAK"
     gene            complement(719..1495)
                     /locus_tag="PK442_10645"
     CDS             complement(719..1495)
                     /locus_tag="PK442_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOO88152.1"
                     /translation="MKFPPHHTFALSAKAADPESRYPWALKHGFALEYTPDPEELELL
                     PAHTAEFRRRNVPVRYHTRYFGLEFGDARAEYAEVAFDAHRRTLDAMAQVGGEVLTVH
                     AGLSKGVTLNSDTVARNLRRLADHGRSLGVTVSLENLRSGPASDPENILRWASSADTA
                     ITMDLGHVVSSEQVKSGELKGEEVARAFLPRLLEVHFYGREEGRHFPLESMKSVEHIV
                     EVVLESSCRWWTVELDNTAEALATKSLLEEYLREKGLLPA"
     gene            complement(1492..1674)
                     /locus_tag="PK442_10650"
     CDS             complement(1492..1674)
                     /locus_tag="PK442_10650"
                     /inference="COORDINATES: protein motif:HMM:NF019998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOO88153.1"
                     /translation="MIRFIIDVGAERPLVVEESAASARTASPGERVSLRFSPEDLRLF
                     GNTSPEAAPMKKEATP"
BASE COUNT          286 a          572 c          630 g          371 t
ORIGIN      
        1 gcgtccttca tggtgaagga cgcccatctc ttcaacggcg tggccgcgtt cgtcgtcgtc
       61 gccttcgcgg tgaactacgc gctcgggatg ttcaagcccg gcttcgaggg ccagccggtc
      121 gcgcacacta accagatgtg gaacttcctc ggcatggtcc tctcgggact cgccttcacg
      181 ctcgcgggag gctgccccgg acgtcaggtg atcatgtccg gcgagggcga cggcgactcg
      241 gcgatctttg tgctcgggat gctcgtcggc gcggggttcg cgcataactt ctcgcttgcg
      301 agttcgcccg cgggcgtgac ggcgtacgga atgacggcga cggcgatcgg catggtcttc
      361 tgcctggccg tcggtttcct gttccgcatc aagatggatt gacgaggagg aatgtgaaaa
      421 tggccgatac tgtgatcgtc gacgcccgcg gactgtcgtg cccgcagccc gtcctcgaga
      481 cgaagaaagc gctggagaag ctctccggcg ggcgcgtgga ggtgctcgtg gacaccgtga
      541 cgtcgcgtga gaacgtcgcc cgcttcggcg gaagccgcgg ctggaaggtg gagaaagagg
      601 agcgcgaaga cggtttctgc gttgtcctcg cgaagtagtt cagcgtgaga aaaacatgcg
      661 gcgctcttcg gagaaaactc cggagagcgc cgttttgttg tccgtcgagg cagccgcgtc
      721 aggcgggcag gagacctttt tcccgcagat actcttccag cagcgatttt gtcgccagcg
      781 cttcggcggt gttgtccagc tcgacggtcc accagcggca cgacgactcc agcacgacct
      841 ccacgatgtg ttcgacgctc ttcatgctct ccaacgggaa gtgccgcccc tcttccctgc
      901 cgtagaagtg cacctccagc aggcgcggca ggaaggcgcg ggcgacctcc tcccccttca
      961 gctcgcccga cttcacctgt tcggaggaga cgacgtgccc caggtccatg gtgattgcgg
     1021 tatcggcgga ggaggcccag cggaggatgt tctccggatc gctcgcgggg cccgagcgca
     1081 gattttccag gctgacggta acgccgagcg atcttccgtg gtcggcgaga cgccgcaggt
     1141 tccgcgcaac cgtgtcggag ttcagcgtca ctcccttgga gagtccggcg tggacggtga
     1201 gcacctcgcc gccgacctgg gccatcgcgt cgagcgtgcg gcggtgcgcg tcgaaggcta
     1261 cttccgcgta ctcggcgcgc gcgtcgccga attcaaggcc gaagtagcgc gtgtggtagc
     1321 gcaccgggac gttgcggcgg cggaattcgg ccgtgtgcgc cggcagaagc tccaactctt
     1381 ccgggtctgg cgtgtactcc agcgcgaagc cgtgcttcag cgcccagggg tagcggctct
     1441 cggggtccgc ggcctttgcg gagagggcga acgtgtggtg cggcggaaat ttcatggcgt
     1501 agcctccttt ttcatcgggg cggcttccgg agatgtgttt ccgaacagcc ggaggtcctc
     1561 cggggagaag cgcagcgaga cgcgctctcc gggggaagcg gtgcgcgcgg atgcggcgct
     1621 ctcttccacg acaagcggcc gctccgcccc cacgtcgatt ataaagcgaa tcacggggcc
     1681 gataaagggc ctgccttctg ggccggcgcc tgcttctgca taagcgttgc cgttcctgcc
     1741 cgcccttttt tgagatttct cggtcctttc gtgttttttg cttctatggc cttcccgctc
     1801 gcactgcttc tgggtcttga gggaattcta cgcttccggg acgtccgggg agaaaatgt
//
LOCUS       DAOSWW010000577         1854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53071_length_1854_cov_2.918844, whole genome shotgun sequence.
ACCESSION   DAOSWW010000577 DAOSWW010000000
VERSION     DAOSWW010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53071_length_1854_cov_2.918844"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PK442_10655"
     CDS             <1..505
                     /locus_tag="PK442_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO88154.1"
                     /translation="ELHPLARELGVAIIGMKPFAGGAITDASLAFKFLRSHADLFPIP
                     GYDSVESVEQVVSFYDAPNSASEKDFEEMAKVKAELGGEFCRRCEYCQPCPFGVAITG
                     SMIYPLLASRMSPKVASNFAAANLDTVPNCTECGVCVTRCPYTLPIPAMLKKHYELYL
                     EHRNAKE"
     gene            513..1388
                     /locus_tag="PK442_10660"
     CDS             513..1388
                     /locus_tag="PK442_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO88155.1"
                     /translation="MADRPPVSPWFVLSLGVFAISTGAIFARMAEAPPLVIAAYRVGL
                     ATLFLLPFTARRAVREISSLDRSDILRGVASGVFLALHFATWISSLFYTSVASSVVIV
                     NAIPLWTGLLSPFLTGDPFSPSLKKGLLLALPGAVVISWGDFALGWSALWGNFLALQG
                     SFFAALYILLGRKVRPRISLGSYVTLNYGTAAVVLWIVVLAFGLPVAGYSTGTWSAFF
                     LMALVPQLLGHSSYNWALRWLTGGTVALCLLGEPVGSSLLAALFLGEPITLVKVLGGG
                     LILAGIYLASKESGE"
     gene            complement(1451..>1854)
                     /locus_tag="PK442_10665"
     CDS             complement(1451..>1854)
                     /locus_tag="PK442_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOO88156.1"
                     /translation="FSDWEQSVVSFVRKGAGGEMVFAVCNFTPVPRFGYRISVPEGGW
                     WRELLNSDAAEYGGGGVGNFGGAKAEPIPNSSWHSLTISLPPLGMLLFKLERPEALPE
                     PVAANRPVSIPVTLPEPAKPPLPEATRRGKK"
BASE COUNT          291 a          622 c          578 g          363 t
ORIGIN      
        1 cgaactgcac cctctcgcgc gcgaactcgg cgtggccatc atcggcatga agcccttcgc
       61 gggcggcgcg atcacggacg cctccctggc cttcaaattc cttcgttccc acgcggacct
      121 cttcccgatt ccggggtacg actcggtgga gtcggtggag caggtcgtct cgttctacga
      181 cgcgccgaac agcgcctccg aaaaggactt cgaggagatg gcaaaggtga aggcggagct
      241 gggcggagag ttctgccgcc gatgcgagta ctgccagccc tgtcccttcg gcgtcgccat
      301 taccggcagc atgatctacc cactgctggc gagcaggatg tcgccgaagg tggcctccaa
      361 cttcgcggcg gcgaacctgg acaccgtccc caactgtacg gagtgcggcg tctgcgtcac
      421 ccgctgcccc tacacgctgc cgattccggc gatgctgaag aaacactacg aactctacct
      481 cgaacaccga aacgcgaagg aataacagcg ccatggccga cagaccgccc gtttcgccgt
      541 ggttcgtcct ctcgctgggg gtcttcgcga tttctaccgg cgccatcttc gcacggatgg
      601 ccgaagcgcc cccgctcgtc atcgccgcct acagggtcgg attggcgacg ctctttctgc
      661 ttccattcac ggcgcggcgg gcggtgcgcg agatctcctc gctcgacaga agcgacattc
      721 tgcgcggcgt cgcttccggg gtctttctgg cgctccactt cgccacgtgg atctcctcgc
      781 tcttttatac gtccgtggcc agcagcgtgg tcatcgtgaa cgcaattccg ctgtggacgg
      841 ggctgctctc gcccttcctg acgggagacc cgttctcccc gtcgctgaag aaggggctgc
      901 tgctggcgct tccgggagcc gtcgtcatca gctggggcga cttcgcgctg gggtggtccg
      961 cgctgtgggg gaacttcctc gcgctccagg ggagcttctt cgccgctctg tacattctct
     1021 taggcaggaa ggtcaggccg cgcatctcgc tggggagcta cgtgacgctg aactacggca
     1081 cggcggccgt cgtcctctgg atcgtcgtgc tcgccttcgg cctccccgtc gccggctact
     1141 cgacaggaac gtggagcgcg ttcttcctta tggcgctcgt gccgcagctg ctggggcatt
     1201 cgagctacaa ctgggcgctc cgctggctga cgggcggcac cgtcgcgctc tgtctgctgg
     1261 gggagcccgt ggggagttcg ctgctcgccg ccctcttcct cggggaacca atcacgctcg
     1321 tcaaggtcct cggaggaggg ctcatccttg cgggcatcta cctcgcgtca aaggagtccg
     1381 gagaatagcc gcgattcgac gaagaaaaaa cgcggaggag aacgtcgtgt ctcctccgcg
     1441 ctgtatgatg ttactttttt cctcgtctcg tcgcctccgg cagaggcggt tttgccggct
     1501 ccgggagcgt aacggggatg gacacaggcc gatttgccgc gacaggctcc ggaagcgctt
     1561 ccggtcgttc cagcttgaag agcagcattc ccagcggcgg cagcgaaata gtcagcgaat
     1621 gccaggagga gttcggtatc ggctccgcct tcgcgccgcc gaagttgccg acgccgcctc
     1681 ctccgtactc cgccgcgtcg ctgttgagca gctccctcca ccatccgccc tccgggacgg
     1741 agattcggta cccgaatcgc ggcacagggg tgaagttgca gaccgcaaag accatctccc
     1801 ccccggcgcc cttcctgacg aaggagacga cgctctgctc ccagtcggag aagt
//
LOCUS       DAOSWW010000578         1853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53115_length_1853_cov_3.324805, whole genome shotgun sequence.
ACCESSION   DAOSWW010000578 DAOSWW010000000
VERSION     DAOSWW010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53115_length_1853_cov_3.324805"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..806)
                     /locus_tag="PK442_10670"
     CDS             complement(33..806)
                     /locus_tag="PK442_10670"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HOO88157.1"
                     /translation="MRKTTNTVALALALLFFAFSAFAEQPTREFKGSFEPANNGERLL
                     SLLVSLTDPESMELRMDQQPDNDGNIRDFSIFIQGASIGGFRVEKIAIESSFLELNRP
                     SEWRTGERDSLKVRNVLRSNVELVALEKDINAALTSYAGGDWRRISVDLKKGMVSARG
                     NYRSGSLDILAEVSTKLEIRQGKQIWLKDTAIKINHDDQTDVIQKELEKIQPVVDIGQ
                     FPFPVTLAVLNVDEQKLVFSTRTLPKPVKGIFYRYVRGS"
     gene            1069..>1853
                     /locus_tag="PK442_10675"
     CDS             1069..>1853
                     /locus_tag="PK442_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO88158.1"
                     /translation="MKSIRTKIMASFIAATIVFMTIMGVTLYARVDGKVTDLTEDMVL
                     QISKASSVGLSEWLDNMVNLTGAFAGMDVVRSLEWKNAEAFLESQVKKHSRLFEMLFI
                     SGTDGRVKGTGGTTADISQRPYFKELMSGGKQYAISDPVVSLATGNVIFVIAHAVPGA
                     DGKPAGVFAATVLLQNLQGLVDAVRVGETGFGWIVDGGGIVAAHRDKEHIGKFDVRQD
                     AAYTTVWKRMSAKETGYELHKRFDGTPAYVFFAPIAGSPGWTFA"
BASE COUNT          391 a          487 c          560 g          415 t
ORIGIN      
        1 gcaaccatgc tgctgaagtc ggcggggaag gttcaggagc cccggacgta gcggtagaag
       61 atccctttga ccggcttggg aagcgttctc gtcgagaaga cgagcttctg ttcgtccacg
      121 ttgagcacgg caagcgtcac aggaaagggg aactgcccga tatcgacaac gggctgaatc
      181 ttctccagct ctttctggat gacgtccgtc tggtcgtcgt ggttgatctt gatcgcggta
      241 tccttcagcc agatctgctt cccctgccgg atttccagct tcgtggacac ctccgcgagg
      301 atatcgaggg agcctgagcg gtagttcccg cgcgcgctga ccatcccctt cttcagatcg
      361 acggagatgc gcctccagtc gccacccgca taggaggtca gggcagcgtt gatatccttt
      421 tcaagcgcga cgagttccac attgctgcga aggacgtttc tgaccttgag cgaatcccgc
      481 tccccagtcc gccactccga cggacggttc agctccagga aggaggactc gatcgcgatc
      541 ttctccaccc ggaacccccc gatggaagcc ccctggatga agatggaaaa gtcgcgtata
      601 ttcccgtcgt tgtccggctg ctggtccatg cgaagctcca ttgattcagg gtcggtcagc
      661 gaaaccagca aggagagcaa gcgttctccg ttatttgcag gttcgaagct tcccttgaac
      721 tccctggttg gctgctccgc gaaggcggag aacgcaaaga agagcagtgc gagcgcaagc
      781 gcaacggtat tggttgtttt tctcatggtt ttccctcctt gaatgtagaa tgctctatct
      841 atttattgcc ttcggaatgg cgccgggaaa taaacctcct ctcttttccg agactattcc
      901 tcttgcgttt cttaggttat actagcagaa aatcagcgtg cgctctatat ctattttgaa
      961 atgcgggaag aggttgtgag cgatacaggc gtgcaaaaac gccttcgaga atcctgcggt
     1021 gaaggagacg gcgttgtaag agacggctga aaggatggtg gatgggcgtt gaagagcata
     1081 cggacgaaga tcatggcatc atttattgca gcgaccattg tgtttatgac gattatggga
     1141 gtgacgctct acgctcgagt cgacggtaaa gtgaccgacc ttaccgaaga tatggttctg
     1201 cagatttcaa aggcgagttc cgtcggcttg tcggagtggc tggataacat ggtgaatttg
     1261 acgggcgctt tcgcggggat ggacgtggtt cgttcgctcg agtggaaaaa tgcggaagcg
     1321 tttcttgaaa gtcaggtgaa aaaacacagc cgccttttcg agatgctctt catctccggt
     1381 accgacggac gagtcaaggg aacggggggg acgactgcgg acatctcaca gagaccgtat
     1441 ttcaaagagc tcatgagcgg cgggaagcag tacgcgattt ccgacccggt cgtctctctg
     1501 gctaccggaa acgtcatctt cgtcattgcg cacgccgttc cgggcgccga cggcaaaccg
     1561 gccggagtct tcgccgcgac ggtgcttctg cagaacctgc agggtcttgt cgacgccgtc
     1621 cgggtgggcg agaccgggtt cggatggatc gtcgacggcg gcggcatcgt cgccgcgcat
     1681 cgcgacaagg agcatatcgg gaagttcgac gtgcgtcagg atgcggcgta tacgacggta
     1741 tggaagagga tgtccgcaaa agagaccggt tacgagctgc acaaacgatt cgacgggact
     1801 ccggcatacg ttttcttcgc ccctatcgcg ggatcgcccg gctggacgtt cgc
//
LOCUS       DAOSWW010000579         1851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53231_length_1851_cov_3.390869, whole genome shotgun sequence.
ACCESSION   DAOSWW010000579 DAOSWW010000000
VERSION     DAOSWW010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53231_length_1851_cov_3.390869"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..>1851
                     /locus_tag="PK442_10680"
     CDS             225..>1851
                     /locus_tag="PK442_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO88159.1"
                     /translation="MIPKEPTYSDLQQRIAGLEEALRMKSLAEETMREQETRYRAIVD
                     QATSDALLVHDFQGRFLEVNRRACESLGYSKEELLRMSVTDIECDFDLKSAQAEWSRI
                     EPGIPFILYGHQRRKDGTVFPVEIHFGCSFWNGETFYLGLVRDITKRKLTEEALRESE
                     ERFRNMVEGAPDAIYIQIDRKFAYVNPAACRLFGVASAEELLGMPLLERIHPLYHDVA
                     RERMRRLSDERSPQRERIELRFLRMDGSEVWVETSGEPINYQGKYGALGFARDITWRK
                     EAEEALGESEERFRSLFRDSLSVMFLLDPVTGRFLDVNRECGNFYGWPAQELLNMNIM
                     DIHTPGEEALCEIEKAASGEQNRFESRHRLADGSVRDVEVFTSKICVRGECLLYCIVN
                     DITAKKAAQRQVRLLGRAVEQNSESVIITDAAGNIEYVNPAFTQSTGYAAEDVAGRNP
                     RVLKSGEQSEAFYKRMWETILSGETWRGEMHNKRKNGELFWENVAISPILDENGAIAH
                     FIAIKQDITERKKLIESLVRAKEKAEESDRLKTA"
BASE COUNT          457 a          446 c          581 g          367 t
ORIGIN      
        1 gtcatgccga ggactgccct gcggtatgcg atagagaaga tgcctcccga gcgaaagaag
       61 gaagccatga agcgatagcg tgagagaagg agagagaaat cctgtccttg aaacatgttg
      121 taaaaagatg gcgccgcccg tttttactgt tgaacgccga tcctccgcac atccgacgct
      181 ccgtgccgga atgctgcgcg gactgaaaga gagatgaggg aaggatgata ccgaaagaac
      241 cgacgtacag tgatcttcag cagcgtatcg ccggtcttga agaggcgttg cggatgaaga
      301 gtcttgcgga ggagacgatg cgcgagcagg aaacgcgata tcgcgccatt gtcgaccagg
      361 cgacgagcga cgccctgctg gtccatgatt ttcagggccg gttcctggaa gtcaaccggc
      421 gtgcgtgcga aagccttggc tattcaaaag aagaacttct tcgaatgtcg gtgaccgata
      481 tagagtgcga ctttgatctg aagagcgcgc aggccgagtg gtcgagaatc gagccgggaa
      541 tccccttcat tctgtatgga catcaacggc gcaaagacgg tacggttttt ccggttgaaa
      601 ttcattttgg ctgttctttc tggaacgggg agacgtttta tcttggcctg gtgcgcgata
      661 tcacaaagcg gaaactgaca gaagaagccc tgcgcgagag cgaagagcgt ttccggaata
      721 tggttgaagg cgcgcccgac gcgatttata tccagatcga caggaagttc gcctatgtaa
      781 atcccgccgc gtgccgtctt ttcggcgtcg cttccgcgga ggaactcctc ggcatgccgc
      841 ttctggagcg tattcatcct ctctaccatg acgttgcccg ggagaggatg cggcgtctga
      901 gcgatgagcg cagcccgcag cgtgaacgta tagagcttcg ttttttgcgg atggacggga
      961 gcgaagtgtg ggtagaaaca agcggagagc ctatcaacta tcagggaaaa tacggagctc
     1021 tcggtttcgc ccgcgatatt acatggcgga aagaggcgga agaagcgctc ggagagagcg
     1081 aagaacgttt ccgcagtctt ttccgggaca gcctttccgt gatgtttttg ctcgaccctg
     1141 ttacgggacg ctttcttgac gtcaacagag agtgcgggaa cttctacggg tggccggcgc
     1201 aggagcttct gaacatgaac ataatggata tccatacgcc gggcgaagaa gcgctgtgcg
     1261 agatagaaaa agccgcgtcg ggagagcaga accgtttcga atccaggcat cgtctggcgg
     1321 acgggtccgt gcgcgacgtg gaagtgttca ccagcaaaat ttgcgtgcgg ggcgagtgcc
     1381 tgctctactg catcgtcaat gacatcacgg cgaaaaaagc ggcgcagcgc caggtacgtc
     1441 tgctcggcag agcggttgag cagaattcgg agagcgtcat tattaccgat gccgcaggga
     1501 atatagagta tgtcaatccg gcgttcaccc agtccacagg gtatgccgcg gaggatgtcg
     1561 caggaaggaa tcctcgcgtg ctcaagtccg gagagcaaag cgaggctttt tacaaacgca
     1621 tgtgggaaac gatcctctcc ggggagacgt ggagagggga gatgcacaac aaacgaaaga
     1681 acggcgaact cttctgggag aacgtcgcga tctcgcccat tctggatgaa aacggcgcta
     1741 ttgcacattt tatcgcgatc aagcaggata tcaccgagag aaagaagctg atcgagtccc
     1801 ttgtacgcgc caaggaaaag gccgaagaga gcgaccggct gaagacggcg t
//
LOCUS       DAOSWW010000580         1851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53235_length_1851_cov_3.199889, whole genome shotgun sequence.
ACCESSION   DAOSWW010000580 DAOSWW010000000
VERSION     DAOSWW010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53235_length_1851_cov_3.199889"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..970)
                     /locus_tag="PK442_10685"
     CDS             complement(110..970)
                     /locus_tag="PK442_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88160.1"
                     /translation="MERPRCSTTHDIIVFDVDGVLVDTSRSYFEVIVQALDWAWTSLI
                     NGVVDCEGFSERHFATTKTHPAFNDDYDIAWAIINCAATAQNPSLAQSLPAPEEWRLL
                     LDGCKESDVPSWVRSTFGEQIPRDTVRRACEELYFGVTKNEKHTALLSPRKGLWENER
                     PMITIPWSAIPVPAGIYTGRTAKELELALKRLHWEDFPDSMIITSDSGVTKPSPRGLE
                     ILCHTSHASTPLFLGDTESDRQTVHAFGRGTFAAIGNFLSDAEFAFPCPEEALEYFGF
                     LSPSNSSVNL"
     gene            1051..>1851
                     /locus_tag="PK442_10690"
     CDS             1051..>1851
                     /locus_tag="PK442_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOO88161.1"
                     /translation="MLLEIPLNYSEGVLYRQLAEHLEKMIRTGSISSGERLPGTRELA
                     RMLRISRCTVVAAYDVLEENGLVKRTARSGTFAASPLLEGRSSYKGKGDILRFDTECP
                     TRDLLPLRELSELCRGLSLSLLGDALEGSPTEGLPSLRRLLLHHAVLRGIPAHPEEVV
                     VTAGGKDALSTALRSFREHGCSKLWVEELSYGEIRSIAENEKIQLRTLPLLEESTASR
                     LDALSSKDVLYLVPSFQNPTGRTLSHSLRQEILSVRRKKGFFILEDDSY"
BASE COUNT          416 a          463 c          519 g          453 t
ORIGIN      
        1 ctcgggatgg agcaggggag ccgctttagc taccgacgtg tgcgtcgagc tgtggagcag
       61 tattgaaggt gtgtatcggt ggctgatgat gcggaaacag tgacgtcctt cataagttta
      121 cactgctgtt ggatggagag agaaaaccga aatattccaa ggcctcctca gggcagggaa
      181 atgcaaactc cgcatcggag aggaaattcc cgatggcggc aaatgttcct ctgccgaaag
      241 cgtgcacggt ctgccggtcg ctttccgtat ctccaagaaa gagcggcgtc gatgcgtgag
      301 aggtatggca cagaatctca agtccgcgag gagatggttt tgtaactccg gagtcggaag
      361 tgattatcat ggaatccggg aaatcctccc agtgaagacg cttgagtgca agttccagct
      421 ctttggcggt tcgtcctgta taaattcctg cagggacagg aatcgcgctc cacggaatcg
      481 tgatcatcgg acgctcgttt tcccatagtc ctttacgggg cgagagcaac gcagtgtgct
      541 tttcgttttt cgtgacgccg aaataaagtt cttcgcatgc tctgcgtaca gtatccctgg
      601 gaatttgttc cccgaacgta ctccgtaccc aggaaggcac gtcagattcc ttgcatccgt
      661 cgagcaagag tctccattct tcaggcgcgg ggagactttg tgcaagagag ggattctgcg
      721 ccgttgcagc gcagttgatg attgcccagg caatatcgta gtcgtcattg aacgccgggt
      781 gtgttttggt ggttgcgaag tgacgttcag aaaaaccttc gcaatccacg accccattta
      841 taagcgacgt ccatgcccag tcgagagcct ggacgatcac ctcgaaatag gaccgggatg
      901 tgtcgacaag cacgccgtcg acatcaaaaa ctataatatc gtgtgtcgtt gaacatcttg
      961 ggcgttccat cgcaaacgct ccttttcaag tatttttctc ccgaagtatt gtaccagatg
     1021 aaccggcgca gatggaaagg agagggtgtt ttgctgctgg aaataccgtt gaactactct
     1081 gaaggagtac tctaccgtca gctcgccgaa catttggaga agatgatacg aaccggaagc
     1141 atctcttccg gcgagcgtct tccggggaca cgggagcttg ccaggatgct ccggataagc
     1201 cgttgtactg tcgttgccgc ctacgatgtc ctcgaagaga acggtctggt gaagagaacg
     1261 gcgagaagcg ggacttttgc tgcttctcct cttcttgaag ggcggtcttc ttataaaggc
     1321 aaaggggata ttctccgttt cgatacggaa tgtcccaccc gagatctgct ccctctcaga
     1381 gagctttcag aactctgccg cgggctttct ctctccttgc tgggcgacgc acttgaaggg
     1441 agtccgaccg agggattgcc ttcattgaga cgccttcttc tgcaccatgc agttctgcga
     1501 ggcattcctg cgcatccgga ggaagtggtt gtcaccgcag gcgggaagga cgctctttcc
     1561 actgctcttc gttcttttcg tgagcacggg tgctccaaac tatgggtgga agagctgtcc
     1621 tacggtgaaa tacgctcgat agcagaaaat gaaaagattc agttgcgtac ccttccgctt
     1681 cttgaagaga gtacggcgtc ccgccttgac gccctctcct caaaagacgt actgtatctt
     1741 gtgccgagct tccagaatcc aacgggccgg acgttgtcgc attcgctgcg tcaggagata
     1801 ctctctgtca ggagaaaaaa gggttttttc attcttgagg acgacagtta c
//
LOCUS       DAOSWW010000581         1851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53251_length_1851_cov_2.652004, whole genome shotgun sequence.
ACCESSION   DAOSWW010000581 DAOSWW010000000
VERSION     DAOSWW010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53251_length_1851_cov_2.652004"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PK442_10695"
     CDS             <1..1285
                     /locus_tag="PK442_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO88162.1"
                     /translation="KNYIQKRIENEWAELWLGDQIPETVEHSRRHSKRLMEFAANLYR
                     TAEEPLGKMNLCSPRALALLIACIYLHDIGHTAISFPLTIEGNNEDAPPFSLGAFPSS
                     VREVHHLLSAQLIRKKAEELFPSSDETDVTFLKTLVPLLSAHHRGYTTLSFEQGKGKP
                     SEQVKQVGSFLFGTAAFEKTLLPLEEKLKLLTESERGGIGVEQLLDVAALLRIIDGSD
                     VQADRVVDHVYLEARLKRTKEEAEILSKELTTFKKVYPEFAEQLVDLLKHVENINLIA
                     RSFDIPRIQSGEIDEKSKEEMKPHCSAVYKEVFDELRRIKGDSDFPGEVYRKDHGKTT
                     LLSLANRIAFKWEQFLHFYKHQCVGTVFPVCEGNKVTVHIWPNKELATHEKTPLCSLI
                     DDFIKKEWEDACTHPRGPEGESVLLKEIKFEGLMH"
     gene            1393..1806
                     /locus_tag="PK442_10700"
     CDS             1393..1806
                     /locus_tag="PK442_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88163.1"
                     /translation="METVREYIAPHLSADTLRRIQALEEERTSGFCPETSYQERLLTI
                     FRNEKNAPRIKKALNNDFNLSSTISDHFLARLLARHGDTCIDFIMRTVEHGIKLSKKN
                     KTSKVYTGDCAITYDPFQRTLISIYPKRSLHTVRH"
BASE COUNT          530 a          540 c          409 g          372 t
ORIGIN      
        1 caaaaactac atccagaaac gaatagaaaa cgaatgggcc gaactctggc tcggagacca
       61 gatccccgag actgtcgaac acagccgccg tcactccaag cggctcatgg agttcgccgc
      121 gaatctctac cgcacggccg aggaacctct cggaaagatg aatctttgtt caccgcgagc
      181 cctggcgctg ctcatcgcct gcatctacct ccatgatatc gggcatacgg ctatctcttt
      241 ccctttgacc atcgaaggga acaatgaaga tgcgccgccg ttctctctcg gcgcattccc
      301 ctcgtctgtc cgggaagtac atcatttgct ctccgcacaa ctgatccgaa aaaaagccga
      361 agagctcttc ccctcctccg acgaaacaga cgtcacattc ctcaagactc tggttcccct
      421 tctcagcgcc caccacaggg gatacaccac tctgtcgttt gaacagggca aagggaaacc
      481 cagcgaacaa gtaaaacagg tgggcagttt cctctttgga acggctgcat ttgaaaaaac
      541 gctcctacct cttgaagaga aactgaaact gctcacggaa agcgaaagag gaggcatcgg
      601 agttgaacag cttctcgacg tagccgccct gcttcgcatc atcgacggtt cggacgtcca
      661 ggccgaccgc gtcgtcgatc atgtctatct cgaagccagg ctcaaacgaa caaaggaaga
      721 agcggaaatc ttgtcgaaag agttgaccac cttcaagaag gtctatcccg aattcgccga
      781 acagctcgtc gatctcttga agcacgtaga aaatataaac ctgatcgcac gttctttcga
      841 cattccgcgc attcaaagtg gagaaatcga cgaaaaaagt aaggaagaaa tgaaacctca
      901 ctgttctgcc gtctataaag aagtcttcga tgaactgcgg cgaatcaagg gcgattccga
      961 ctttcccgga gaagtctatc gtaaggatca cggaaaaacc acgcttctct cgctcgccaa
     1021 ccgcatcgcc ttcaagtggg agcagttcct ccacttctac aaacatcagt gcgtcggcac
     1081 ggtctttccc gtctgcgaag ggaacaaggt aacggttcac atctggccga acaaagagct
     1141 tgcgacgcat gaaaaaacac ccctctgctc tctcatagat gatttcataa agaaagaatg
     1201 ggaagatgcc tgcactcatc cgagaggacc agaaggagag agcgttcttt taaaagaaat
     1261 aaaattcgaa ggcctcatgc actgacgcag ccctgaatag tttcaccaat atcgtagata
     1321 taagagcgaa agagcgaaca cgcctctttc gctcttgttt tttcttatcc cgataaagga
     1381 ggacggaaaa cagtggaaac agtcagggag tacattgcgc cccatctgag cgccgatacc
     1441 cttcggcgca tccaagctct cgaagaagag cgcaccagcg gcttctgccc cgagaccagc
     1501 tatcaggaac gcctgctcac aatcttccgc aacgagaaaa acgcaccgcg aataaaaaag
     1561 gcgctcaaca acgatttcaa tctctcttcc acgatttcgg accactttct cgcccgtcta
     1621 ctcgcccgcc acggcgatac atgcatcgat ttcatcatgc gcaccgtcga gcacggcatc
     1681 aaactttcga aaaaaaacaa aacgtccaaa gtctacacgg gagactgtgc catcacatac
     1741 gacccgttcc aacgaacact catttcaatc tacccgaaga gatccttgca cactgtcaga
     1801 cactgagcac cgagattgac cgaatatccc cctccagact ccgtaagaca a
//
LOCUS       DAOSWW010000582         1847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53424_length_1847_cov_3.823103, whole genome shotgun sequence.
ACCESSION   DAOSWW010000582 DAOSWW010000000
VERSION     DAOSWW010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53424_length_1847_cov_3.823103"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PK442_10705"
     CDS             complement(<1..885)
                     /locus_tag="PK442_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO88164.1"
                     /translation="MTDFSVVGKPVLKVDSLSLACGSARYTADYEMRDPLYLAFAYSP
                     VAHGDIVSLDDSEARAMEGVTDVFHAFNTETELFTTAGQGYPEPSPYDTRLFNKRVRY
                     AGEIVAAVLAGTLSEARAAAAAVKVEYAPLPTLFDPEKAMDKDSPSLHGEDGAYAPLP
                     VPFRVEENVAGEVLFSFGDMEKGFADADFIVDETYRTHYTAHCALEPHAASAFFDEKG
                     RLVIVASTQVPFHARRTVSRVVGIPAHMIRVIKPRIGGGFGSKQEIILELYAALAAWK
                     YKRNVKAEMSRSEVFTAAR"
     gene            complement(878..1345)
                     /locus_tag="PK442_10710"
     CDS             complement(878..1345)
                     /locus_tag="PK442_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO88165.1"
                     /translation="MKGSFLVNGRTNEWTFEPDATLLDVLRANGHPEVHRGCDEGVCG
                     SCSVLLEGKLVNSCQVFAASAMEKDILTVAGIGTIHEPHPIQTAFVESGAVQCGFCTP
                     AAILATLALLRKNPNPTDEEIHSALDGNYCRCTGYVKIIEAVRLASRRMSSHD"
     gene            complement(1342..>1847)
                     /locus_tag="PK442_10715"
     CDS             complement(1342..>1847)
                     /locus_tag="PK442_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88166.1"
                     /translation="SLFPLWSDLIGPMSVLHGVICTEGKNGGTYDLPSWLADRTLQKG
                     SLITSLLFPDPVDWLSSYYRETRVGFDYPAFTVSILARKNNSTIDKVRIAVSGGLDRV
                     RRLTELEERLTGADLSVLASLDIRKMASVEFGRKPAGSPEYLSEIAAVQVERGLKAVL
                     TEEGKSR"
BASE COUNT          433 a          553 c          494 g          367 t
ORIGIN      
        1 cctcgccgct gtaaaaactt cggaacggct catctccgcc ttgacgtttc gtttgtactt
       61 ccacgccgcc agcgctgcat agagctcaag aatgatctcc tgcttgctgc cgaagccccc
      121 gccgattctg ggttttatca cgcggatcat atgcgcggga atacctacga ccctgctcac
      181 ggtccgtctg gcatgaaaag gcacctgcgt agaagccaca atgacaagac gtcccttttc
      241 gtcgaagaac gccgatgccg catgcggctc cagcgcacaa tgcgccgtat agtgcgtcct
      301 gtatgtttcg tcgacgataa aatccgcatc cgcaaacccc ttctccatat cgccgaacga
      361 gaagagcacc tctccggcaa cattttcctc cacacggaaa gggacaggga gcggcgcgta
      421 cgctccgtct tcaccgtgaa ggcttggaga atccttatcc atagcctttt ccggatcgaa
      481 gagcgtcgga aggggagcat actccacttt caccgcagcg gcggcagccc gcgcttcgga
      541 aagggttccg gcgagaacgg cagcgacgat ctcccccgcg taccgtacgc gcttgttgaa
      601 aaggcgcgta tcgtacggag aaggttccgg gtatccttgc cccgcggtcg taaacaactc
      661 cgtttccgtg ttgaatgcgt gaaagacgtc cgtaacgcct tccatcgcgc gagcctcgga
      721 atcgtcgaga gaaacaatat ccccgtgcgc cacgggcgaa taggcaaaag caaggtagag
      781 cggatcacgc atctcgtaat cggcggtgta gcgggcagaa ccgcacgcca gcgacagaga
      841 gtcaaccttg agaacgggtt tccccacaac agagaaatca gtcatgcgag ctcatcctcc
      901 tggaagcgag acggaccgct tcaataatct ttacgtagcc ggtgcaccgg cagtagtttc
      961 cgtcgagggc ggaatgaatc tcctcatcgg taggattcgg atttttgcgc aacagcgcca
     1021 gcgtcgcgag aatcgcagcc ggagtacaaa aaccgcattg gacggctccg ctctccacaa
     1081 aagccgtctg gatcgggtgc ggttcatgaa tcgttccgat acctgcaacc gtcagaatat
     1141 ccttctccat tgccgatgcg gcgaacacct ggcaagaatt gacgagtttt ccctccagaa
     1201 gcaccgagca ggacccgcac actccctcgt cgcacccgcg gtgcacttcc ggatgaccat
     1261 ttgcgcgaag cacgtcaagc aatgttgcat caggttcgaa cgtccactca ttggtacgtc
     1321 cgttcaccaa gaaagacccc ttcatcgaga cttcccctcc tcagtcagaa cggcctttaa
     1381 gcctctttca acctgtacgg cggcaatctc cgaaaggtat tccggtgatc ctgccggttt
     1441 tctcccgaac tcaacagaag ccatcttccg aatgtcaagc gatgcaagga cagaaagatc
     1501 cgctcccgta aggcgttcct caagctcggt aagacggcgc accctgtcca ggcctccgga
     1561 gactgcaatc cggaccttat ctatcgtact gttgtttttg cgtgcgagaa tagagacggt
     1621 aaaggccgga taatcgaatc cgacacgggt ctctctgtag taggaggaga gccagtcgac
     1681 cggatcaggg aagagaagcg aagtgatcag agagcccttc tgaagagttc tgtcggcaag
     1741 ccaggagggc aggtcgtagg tcccaccgtt cttcccctcg gtgcagataa ctccgtggag
     1801 caccgacatg gggccgataa gatcggacca aagcggaaag agcgacg
//
LOCUS       DAOSWW010000583         1847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53457_length_1847_cov_2.036272, whole genome shotgun sequence.
ACCESSION   DAOSWW010000583 DAOSWW010000000
VERSION     DAOSWW010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53457_length_1847_cov_2.036272"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK442_10720"
     CDS             complement(<1..204)
                     /locus_tag="PK442_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88167.1"
                     /translation="MPILKRTVLFCKPGHVMEPPTDKEFEELDREEAKQNRIEAAKAK
                     LREAARKGDGKAFADLEADIAAME"
     gene            357..581
                     /locus_tag="PK442_10725"
     CDS             357..581
                     /locus_tag="PK442_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88168.1"
                     /translation="MAQFVPLRCRVNFRLLAGSNPETGRDIIRTVSVTRTDPSLTPAA
                     LKEVETLLSPMFEYPVVEVQRALTDLVEDI"
     gene            599..802
                     /locus_tag="PK442_10730"
     CDS             599..802
                     /locus_tag="PK442_10730"
                     /inference="COORDINATES: protein motif:HMM:NF022597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HOO88169.1"
                     /translation="MIRTLRLMFATVEGKTFTVSLNHVKDDMTAPQIEALMEQMLDSE
                     MFAEGLAGLKGADLIARTQTVYF"
     gene            816..1046
                     /locus_tag="PK442_10735"
     CDS             816..1046
                     /locus_tag="PK442_10735"
                     /inference="COORDINATES: protein motif:HMM:NF019487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HOO88170.1"
                     /translation="MAAIFDKTSNRMTLRLNTGADPETGKAILKTVGFAIVPTATADQ
                     TNAAKTALAPLLAHPTIAVEVSGVEVLGDDGN"
     gene            1062..1259
                     /locus_tag="PK442_10740"
     CDS             1062..1259
                     /locus_tag="PK442_10740"
                     /inference="COORDINATES: protein motif:HMM:NF022597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HOO88171.1"
                     /translation="MKTLRMKFVDTDGKAKMISLNYARENVTAGEVEDAMDTLMTQSL
                     VPGAVGLSGADLVDRTVTPLF"
     gene            1270..1494
                     /locus_tag="PK442_10745"
     CDS             1270..1494
                     /locus_tag="PK442_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HOO88172.1"
                     /translation="MTSAATPTTDFLTAIASAGFPVAVAAFLLMRLEKRLESLTRAIE
                     GLRRCQVCRLDEPDAQERAALRLRAVGGGK"
     gene            1495..>1847
                     /locus_tag="PK442_10750"
     CDS             1495..>1847
                     /locus_tag="PK442_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HOO88173.1"
                     /translation="MPWTFTPRHFEAYEFGCRCGCGAGSMAAALLLRLDAAREAFGRP
                     IYVTSGRRCAAHNASAAVGGAPQSRHLIGCAADVRPPQGASLSELAALLALYFSTPAD
                     EYIVYPSKGIVHVAVS"
BASE COUNT          353 a          605 c          534 g          355 t
ORIGIN      
        1 ctccatcgcg gcgatatcgg cctccagatc ggcgaaagcc ttgccgtcgc ccttccgtgc
       61 ggcctcgcgg agcttcgcct tggcggcctc tatcctgttc tgcttggctt cttccctgtc
      121 cagctcttcg aattccttgt ccgtcggagg ctccatcacg tgtccaggtt tgcagaagag
      181 aacggtgcgc ttgagaatcg gcattgagaa atacccccta tatgatgttt tataactcaa
      241 atataccaca catatagtgt ctttcgtata gacaactttt ttaagatgtg atatcatgcg
      301 ctcatccacc cgaggaggcc ggagggacac tttttctcgc aaaggagaga agacccatgg
      361 cccaattcgt tccgctccgc tgtcgcgtca acttccgtct gctcgccggc agcaaccccg
      421 agactggacg ggacattatc cggacagttt ccgtcacccg caccgatcca tcgcttaccc
      481 ccgcggcgct caaagaagtc gaaactctgc tttccccgat gttcgagtac cccgtcgtcg
      541 aagtgcagcg cgcgctcacc gacctcgtgg aagatatcta aggaagggga gatagcagat
      601 gattcgtact ctcagactga tgttcgccac cgtcgagggg aagacgttca cggtctccct
      661 gaatcatgtg aaggacgaca tgaccgcccc tcagatcgag gcgctcatgg aacagatgct
      721 cgactcggag atgttcgccg agggactggc cggactcaag ggcgccgatc tgatcgcgcg
      781 cacccagacc gtgtatttct aagggagtga gataaatggc cgccattttc gacaagacgt
      841 ccaaccgaat gacgctcagg ctcaacaccg gagcggatcc cgagaccggg aaagcaatcc
      901 tgaagaccgt aggcttcgcg atcgttccga ccgcgacggc ggatcagacg aacgccgcaa
      961 aaaccgcgct tgctccgctc ctcgcccatc cgaccatcgc cgtagaagtc tccggggttg
     1021 aagtcctcgg cgacgacggc aattaagaga ggtgacagca gatgaagact cttcgcatga
     1081 aattcgtcga caccgacggg aaagccaaga tgatctccct caactacgcg cgcgagaatg
     1141 tcacggcggg cgaggttgag gacgccatgg acacgctgat gacgcagtcg ctcgttccgg
     1201 gcgcggtcgg tctttccggg gccgatctgg tcgatcgcac ggtaacgccg ctcttctagg
     1261 agcagtttca tgacgtccgc cgccactccg accaccgact ttctcaccgc gatagcctcc
     1321 gccgggttcc cggtcgcggt ggcggcgttc ttgctcatgc gccttgaaaa gaggctcgaa
     1381 tcgctgacgc gggccatcga ggggctgcgc cgctgccagg tctgccggct cgacgaaccg
     1441 gacgcccagg agcgcgccgc cctccgcctg cgcgccgtcg gcggagggaa gtagatgccc
     1501 tggacgttca ccccccggca cttcgaagcg tacgagttcg gatgccggtg cggctgcggg
     1561 gccggttcaa tggccgccgc gctgctcctt cgcctcgacg ccgcaagaga ggccttcgga
     1621 cggccgatct acgtcacctc cggacgccgg tgcgcggcac acaacgcgag cgccgccgtg
     1681 ggcggggccc cgcagtcccg ccacctgatc ggatgcgccg ccgacgtgcg cccgcctcag
     1741 ggcgcatctc tttcggagct ggccgcattg ctggccctct acttttcgac gcccgccgac
     1801 gaatacatcg tctacccctc gaaggggatc gtccacgtcg ccgtctc
//
LOCUS       DAOSWW010000584         1846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53476_length_1846_cov_3.415410, whole genome shotgun sequence.
ACCESSION   DAOSWW010000584 DAOSWW010000000
VERSION     DAOSWW010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53476_length_1846_cov_3.415410"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PK442_10755"
     CDS             <1..301
                     /locus_tag="PK442_10755"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="catalyzes the formation of alpha-D-galactose
                     1-phosphate from D-galactose in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOO88174.1"
                     /translation="SPLCHESSARQSSSLHVSLQFDYETTGRELDCLAELSWQSGECL
                     GARMTGAGFGGSALAIVRSDGVDAFTRFVGERYRRYIGYDCSFFRSVAGDGARRI"
     gene            complement(347..1195)
                     /locus_tag="PK442_10760"
     CDS             complement(347..1195)
                     /locus_tag="PK442_10760"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOO88175.1"
                     /translation="MIARLSKKRALIVLFLGVLLTSIFLFAGSAAAEPTLLEIYDTLK
                     TKEFVDLTHTFDTEIPHWPGFPKEKRETIYWYGKGVGTMGYGFFAQVFCHVGQWGTHV
                     DAPAHFVRGTRTLDQIDVKEMLLPLVVIDVHEKAAANPDYTLTLDDVKEWEKKYGPVP
                     EGAFVAMRTDWSKKWPDAAAMRNADQAGTAHYPGWSLEAMKYLFEERKITASGHEPTD
                     TDPGVSTSKGDYTCESYVLGQDKYQIELLANLDKVPEHGAIVVVAFPKPFFGSGFPAR
                     VFAILP"
     gene            complement(1564..>1846)
                     /locus_tag="PK442_10765"
     CDS             complement(1564..>1846)
                     /locus_tag="PK442_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88176.1"
                     /translation="FAYVKTKKWSELMAIQEDILFRIGEIVEKAGSGFAFPSATVYTA
                     KDDGIDKEKGAAVEKVVQQWRGEDLYPFPDTPPERLAPLLGTIEYPPKS"
BASE COUNT          342 a          518 c          551 g          435 t
ORIGIN      
        1 ctcgccgctc tgccacgaaa gttcggcaag gcagtcgagc tctcttcacg tgtcattgca
       61 gttcgactac gaaaccacgg gaagagagct cgactgcctt gccgaacttt cgtggcagag
      121 cggcgagtgc cttggcgcac ggatgaccgg cgccgggttc ggaggaagcg cgcttgccat
      181 tgtccggagc gatggagtgg atgcgttcac tcgttttgtg ggagagcggt accggcggta
      241 tatcggctac gactgttcct tcttcaggag cgtcgccgga gatggagcgc gcagaatata
      301 gttcgggcgc cctctattcc aggggcgccc gcgttgtttc ctgagcttag gggagtatag
      361 cgaagactct cgcggggaat cccgagccga agaacggctt ggggaaggcg accacaacga
      421 tcgcgccgtg ctcgggaacc ttgtccagat ttgccagaag ttctatctgg tacttgtcct
      481 gtcccagtac gtacgactcg caggtgtagt cgcccttcga ggtcgacacg ccggggtccg
      541 tgtccgtggg ttcatgtccg gatgcggtga tcttccgttc ctcgaagagg tacttcatgg
      601 cttcaagact ccatcccggg tagtgcgccg ttcccgcttg gtccgcgttt cgcatcgccg
      661 cagcgtccgg ccatttcttc gaccagtcgg tgcgcatcgc gacgaatgct ccttcaggaa
      721 cggggccgta cttcttctcc cactccttta cgtcgtcgag cgtgagcgtg tagtccggat
      781 ttgccgccgc tttttcgtgg acgtcgatca ccacgagagg aagcagcatc tcctttacgt
      841 cgatctggtc gagggtcctg gtcccccgga cgaagtgggc gggagcgtcg acgtgcgttc
      901 cccactgccc gacatggcag aagacctggg cgaagaagcc gtagcccatc gtgccgacgc
      961 ctttgccgta ccagtagatc gtctcccgtt tctccttcgg gaatcccggc cagtgcggaa
     1021 tctcggtgtc gaaggtgtgc gtcaggtcga cgaactcctt cgtcttgagg gtatcgtaga
     1081 tttcgaggag cgtgggctcg gcggccgccg aacctgcgaa gagaaagatg cttgtaagca
     1141 gtacgccgag aaagagaact atcagcgctc ttttctttga caatcgagcg atcatttttc
     1201 gacctccgtt tccagtctat ccggtacccc tgaacgcttc gccctccgga gaaaaccaca
     1261 atctcttcgg gggacggagg actgctgaac acaaattgca cacgctttgt tggaagaatc
     1321 cgtcgcatcc ctccctccct tcttcttttc gactgcttgt tttgaaataa cgcactattt
     1381 acttttcatc ggtactctac caaaaagagc gcgtttttgc aacagcgaag cattgtcttg
     1441 tcacactgcg atgctggcaa caaattgttg agagagtctt tgaggggcaa attcagaagg
     1501 aagggcgggg cggcggccgc accttacggg cggttacagc gtaaggtgcg gctctcaaga
     1561 aggctaggat ttcggagggt attctatggt tccaagaagg ggtgcgagtc gctccggagg
     1621 cgtgtctggg aacgggtaga ggtcctctcc tcgccactgt tgcaccactt tttcgactgc
     1681 ggcgcccttt tccttgtcga tgccgtcgtc tttggctgtg tacaccgtag cagaggggaa
     1741 ggcgaatccc gatccggcct tctcgacgat ctcgccgata cggaagagga tgtcctcttg
     1801 gattgccatc agctcggacc acttcttcgt cttgacgtag gcgaag
//
LOCUS       DAOSWW010000585         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53562_length_1844_cov_3.962549, whole genome shotgun sequence.
ACCESSION   DAOSWW010000585 DAOSWW010000000
VERSION     DAOSWW010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53562_length_1844_cov_3.962549"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK442_10770"
     CDS             complement(<1..188)
                     /locus_tag="PK442_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88177.1"
                     /translation="MASPSSLGDALTKQAEILRTLGEVTVRQREALKEGRLELLQDLF
                     REQQAAGFAAEAAEERRRR"
     gene            complement(191..439)
                     /gene="flgM"
                     /locus_tag="PK442_10775"
     CDS             complement(191..439)
                     /gene="flgM"
                     /locus_tag="PK442_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOO88178.1"
                     /translation="MDKLNAKKGYKPSREGGKGDGVEFSSFAVALAKVSGELKKIPDV
                     REQLVEDFKKQIAEGTYSPDMNQVARSLIGAGLLNRED"
     gene            complement(516..1166)
                     /locus_tag="PK442_10780"
     CDS             complement(516..1166)
                     /locus_tag="PK442_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOO88179.1"
                     /translation="MSTLEELFRFTAHLFWPRACPFCGALGESACVLCLLPCLEPPMP
                     MELGSLPLWSGGEHDGVLRELVLALKYGGDRSLGVAMGRALGRIFPRPDADLLVPVPL
                     HRGSERAYNQSLALAAGAAKEWGIPAKEGLVWSERREEQTSLGQEERKMMPRDVFSPV
                     TKSVAGKRIVLVDDVATTGTTLERAAETVRRGGGDPVLALSWTCAPRTKERMRGVR"
     gene            complement(1153..1572)
                     /locus_tag="PK442_10785"
     CDS             complement(1153..1572)
                     /locus_tag="PK442_10785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO88180.1"
                     /translation="MNGGYFFYDSKNAVLASELANLYRFTRWGRSRSVEDIDRMLRGT
                     SMCFSVRYEEHLVAFCRILTDFIYRGSLWDIMVHPDHQGKKVGTALLDYALNHPAIKP
                     IPLIITYTSELAPFLVPHGFAPKEGALMLLRRPIEYS"
     gene            complement(1569..1787)
                     /locus_tag="PK442_10790"
     CDS             complement(1569..1787)
                     /locus_tag="PK442_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HOO88181.1"
                     /translation="MLIVACQICGETKVIAGTPDGDGMARATWICPVCGTGQVMQLPV
                     SSDARKSDLRSIVCGMALATHGGMEERP"
BASE COUNT          347 a          564 c          549 g          384 t
ORIGIN      
        1 cgtctgcgcc gctcttcggc ggcttccgcg gcgaaccctg ccgcctgctg ctcgcggaag
       61 aggtcctgta ataattcgag gcgcccttct ttgagcgcct ctctctgccg tacagtaact
      121 tcaccgagcg tgcgcagaat ttccgcctgc ttggtcagcg cgtctcccag agaggacggt
      181 gaggccacgg ttaatcctcc cggttcagca gccccgcgcc gataaggctt cgcgccactt
      241 ggttcatatc cggtgagtag gttccctcgg ctatctgttt tttgaaatcc tcgacgagct
      301 gttcgcgaac gtcgggtatc ttttttaact cgccggaaac tttagcgagc gcgacggcaa
      361 aggagctgaa ctctactccg tcgcctttgc ccccctcgcg ggaaggtttg taccctttct
      421 tcgcgttaag tttgtccatt acgtttggtc cgtacacgcc gggaatctta tcgatcattt
      481 ttatcacccc gaaagaaact tttctcttca ctttgttatc ggacgccgcg catcctctct
      541 ttagtccggg gcgcgcaggt ccacgaaagt gcaaggacgg gatcgccccc gccgcgacgt
      601 actgtttcag cggctcgttc caaggttgtg ccggttgtgg cgacgtcgtc cacaaggacg
      661 attctctttc ccgcaacgct cttcgtcaca ggcgaaaaga cgtcgcgggg catcattttc
      721 cgttcctcct gccccagaga ggtctgttct tcacggcgtt cgctccagac gagcccttct
      781 ttggcaggaa tcccccattc ttttgccgcg cctgcggcaa gagcaagaga ctggttatag
      841 gctcgttcgc tcccccggtg cagcgggacg ggaacgagga ggtcggcgtc cggacggggg
      901 aagatgcgcc ccagcgcacg gcccatagcg acgccgagcg accggtctcc tccgtacttc
      961 agcgcgagga caagctcccg gagcacgccg tcgtgctctc cgcccgacca gagcgggagg
     1021 ctcccaagct ccatcggcat cggaggctca agacagggca acagacagag aacgcacgcg
     1081 gactcgccga gcgcgccgca gaaggggcac gctctgggcc agaagaggtg cgcggtaaag
     1141 cggaaaagct cctcaagagt actcaatcgg ccgcctcagg agcatgaggg cgccctcttt
     1201 cggggcgaag ccgtgcggaa cgagaaaggg ggcgagttcg ctggtatagg tgatgatcag
     1261 cggaatcggc ttgatcgccg ggtggttgag cgcatagtcg agcagcgcgg ttcccacctt
     1321 ttttccctga tgatcagggt gtaccatgat gtcccagaga gatccccggt agataaagtc
     1381 ggtcagtatc ctgcagaaag cgacaaggtg ctcctcgtaa cgcacgctga agcacatcga
     1441 ggtgccgcga agcatgcggt cgatgtcctc gacggagcgg cttcgccccc atcgggtaaa
     1501 gcggtagaga ttggccagct cggaggcgag caccgcgttc ttgctgtcgt aaaaaaagta
     1561 gccgccgttc acggcctttc ctccatacct ccgtgcgtcg caagggccat cccgcagacg
     1621 atggagcgga gatcggattt ccgggcgtcg gaggagaccg gaagctgcat gacctgcccc
     1681 gtgccgcaaa ccggacagat ccaggtggct cgggccattc cgtcgccgtc gggcgttccg
     1741 gcgatgacct tcgtttctcc gcaaatttga caggcaacta tgagcattgc gcattcctcc
     1801 acagggaact aattttcttc ggcagggcgt atctcgatgg agac
//
LOCUS       DAOSWW010000586         1842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53663_length_1842_cov_3.538332, whole genome shotgun sequence.
ACCESSION   DAOSWW010000586 DAOSWW010000000
VERSION     DAOSWW010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53663_length_1842_cov_3.538332"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PK442_10795"
     CDS             <1..1360
                     /locus_tag="PK442_10795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO88182.1"
                     /translation="DYQTEGFQWLCRLAHWGAGACLADDMGLGKTIQVLAVLLSRAQT
                     GPSLVIAPVSVCPNWFQEAARFAPTLNLKEFRYGDRQKILDDLEPFDVVVTSYGLLQQ
                     DGEMLHEVEWNTVVLDEAQAIKNAATKRSAAAMRLKGKFRIITTGTPIENHLGELWNL
                     FRFLNPGLLGSLDSFNRRFATPIVKYDDKGARARLKKILRPFLLRRTKDQVLDELPPK
                     TEITLRVDMNPEERSLYEALRRNALERIEKGDGEDQRFVILAELMKLRRACCNGALVL
                     PDREFPSAKLEAFGEILEDLRANGHRCLVFSQFVDHLAILRASLNEAGVSYSYLDGST
                     PPEERKRQVEAFQAGEKECFLISLRAGGTGLNLTAADYVVHMDPWWNPAVEDQASDRA
                     HRIGQERPVTVYRIVAKDTIEEKIVDLHATKRDLAESLLEGTERVTSFSLNEMAALIS
                     QE"
     gene            complement(1415..>1842)
                     /locus_tag="PK442_10800"
     CDS             complement(1415..>1842)
                     /locus_tag="PK442_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOO88183.1"
                     /translation="RKCLAEAIMAFYPKYSLEDLVVPTSEIPKLMAETTRLCTKYGLE
                     EANFGHVGDGNMHVNILFPEERPDWHGIVDELLDELYDFTASIGGTLSGEHGIGLKRQ
                     KYMCRVMDASQMELIHRVKLAFDPNNILNPGKMISCPDR"
BASE COUNT          355 a          588 c          563 g          336 t
ORIGIN      
        1 cgactatcag accgaaggat tccagtggct ctgccgcctc gcgcactggg gcgcgggcgc
       61 atgcctcgcg gacgatatgg ggctcggcaa gaccatccag gtgctcgccg tccttctctc
      121 ccgcgcgcag accggtcctt cgctcgtgat cgctcccgtc tccgtctgcc cgaactggtt
      181 tcaggaggct gcgcgttttg cgcccactct gaacctgaag gaattccgct acggcgaccg
      241 gcagaagatc ctcgacgacc tggagccgtt cgacgtggtg gtgacaagct acgggctgct
      301 tcaacaggac ggagagatgc tccatgaagt cgagtggaac accgtggttc tcgacgaggc
      361 gcaggccatc aagaacgctg cgacgaagcg ctcagccgcg gcgatgcggc tgaaggggaa
      421 attcaggatt atcaccaccg gcaccccgat cgaaaaccat ctcggcgagc tgtggaacct
      481 cttccgcttt ctcaatccgg ggctgctcgg ctcgctcgac agcttcaacc gccgcttcgc
      541 cacaccgatc gtgaagtacg acgacaaggg agcgcgcgcc cggctgaaga agatcctccg
      601 ccccttcctg ctccgccgca ccaaggacca ggtgctcgac gagctgcctc cgaagacgga
      661 gatcaccctc cgcgtggata tgaacccgga ggagcgaagc ctctacgagg cgctcaggag
      721 gaacgcgctc gaaagaatcg agaagggaga cggagaggat cagcgcttcg ttatcctcgc
      781 cgagctgatg aagctgcggc gcgcatgctg taacggcgcg ctcgtcctcc ccgacagaga
      841 gttcccctcc gcgaaactgg aggcgttcgg cgagatattg gaagaccttc gggcaaacgg
      901 acaccgctgc cttgtcttca gccagttcgt cgaccatctg gccatcctgc gagcatcgct
      961 gaacgaagcc ggagtttcgt actcatacct cgacggttcg accccgcccg aggaacggaa
     1021 acgccaagtg gaagcgttcc aggccgggga gaaggagtgt tttctcatca gcctgcgcgc
     1081 cggaggaacg gggctgaacc tcacggccgc cgattacgtc gtccacatgg acccctggtg
     1141 gaaccccgcc gtcgaggatc aggcctccga ccgtgcgcac cgcatcgggc aggagcgtcc
     1201 cgtgacggtc taccgcatcg tggcgaagga taccatcgag gagaagatcg tagacctgca
     1261 cgcgacgaag cgagatctcg ccgaaagcct gctcgaagga acggagcgcg tgaccagctt
     1321 ctcgctcaac gagatggccg cgctcatcag ccaggaatga acaacggggg ccttctcggg
     1381 cccccgttgc gttccgcttc tccggtgtgt cctactaccg atccgggcag ctgatcatct
     1441 tgcccgggtt gaggatgttg ttcgggtcga aggcgagctt cactctgtgg atcagctcca
     1501 tctgcgaggc gtccatcacg cggcacatgt acttctgccg tttcaggccg ataccgtgtt
     1561 cgccgctcag cgtgccgccg atggaggccg taaagtcgta cagctcgtcg aggagttcgt
     1621 cgacgatgcc gtgccagtcg gggcgttcct cggggaaaag aatgttcacg tgcatgttgc
     1681 cgtcccccac gtggccgaag ttcgcctcct ccaatccgta tttcgtacaa aggcgcgtcg
     1741 tctcggccat cagcttcgga atctcgctgg tgggcaccac caggtcttcg aggctgtact
     1801 tcggatagaa ggccatgatc gcctcggcca agcactttcg cg
//
LOCUS       DAOSWW010000587         1840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53779_length_1840_cov_3.363025, whole genome shotgun sequence.
ACCESSION   DAOSWW010000587 DAOSWW010000000
VERSION     DAOSWW010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53779_length_1840_cov_3.363025"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..766
                     /locus_tag="PK442_10805"
     CDS             101..766
                     /locus_tag="PK442_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOO88184.1"
                     /translation="MIGNLGILTLAAQLNADGFEAYAYTGITTDVVKTIGQMKERLFA
                     VCFYCDFDNQSAVEAIIKGLKNEAGNNFYVVLGGPHTLHMTERDLERYGADAIIKGEG
                     EESLPAWLRARRTDTTVAVPGEMLPGEPSEYVYLKDFSHYAPPQDEAIVGYGERPLMS
                     VITARGCPYRCAFCFEGGNSKNLRMRTRTTCCVKSKRGCRKAGGHGICFFATTPSPPI
                     PSA"
     gene            670..1410
                     /locus_tag="PK442_10810"
     CDS             670..1410
                     /locus_tag="PK442_10810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO88185.1"
                     /translation="MLCEIEARLQKSRGPRYLFFCDDTFTANPERLSELLKGVRRLRE
                     RHDFVWFCEGHPGFLARRPELIAEMIASGMVRMQIGMESGDGRVLQAYGKQAVPEDVK
                     NVVDTCYREGLPQLAGNFIIGGAFETRRTLETTTGFALDLLHRAPGMLDLSTTFLMPL
                     PGTRISTHPGSFGIALEDRDCLTSIEDFPVNSTLELSVPEICRERSVFLTHISNAMKA
                     QYANGRIPPERIRRDFELAFRYGMRRGT"
     gene            1569..1721
                     /locus_tag="PK442_10815"
     CDS             1569..1721
                     /locus_tag="PK442_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88186.1"
                     /translation="MSGAETDILLDAGSSSVKETAKKLNLSIEATSVCLERLSAQHLT
                     LFSPCL"
BASE COUNT          407 a          513 c          544 g          376 t
ORIGIN      
        1 tggaacaaaa gaaatggatt ctctaccaga aaccatgaac gcgtctcccc ttgttctcct
       61 cgtgtacacc caacggctgg tcccggggag gacctttgaa atgatcggga atctgggaat
      121 tctcacgctc gcggcgcagc tcaacgccga cggttttgaa gcatacgcct ataccggcat
      181 cacaaccgat gtggtcaaaa ccattggaca aatgaaagag cgccttttcg cggtctgttt
      241 ctactgcgac ttcgacaacc agtccgcagt ggaggcgatc atcaagggtc tgaaaaatga
      301 agccggaaat aatttctatg ttgtcctggg cggaccgcat acgctgcaca tgacggaacg
      361 ggatctggaa cggtacggcg cggacgccat catcaagggg gaaggggaag agtccctccc
      421 cgcctggctc cgggcccgcc gtacggacac aacagtcgcc gtgccggggg aaatgcttcc
      481 cggggagcct tccgaatacg tgtatctgaa ggatttttct cactacgctc cgccgcagga
      541 tgaagcgata gtaggatacg gagaacggcc cctgatgtcg gtgatcacgg ccaggggctg
      601 tccgtaccgc tgcgcattct gtttcgaggg gggcaattcg aaaaacttgc gaatgcgcac
      661 ccggacgacg tgctgtgtga aatcgaagcg cggttgcaga aaagcagggg gccacggtat
      721 ctgttttttt gcgacgacac cttcaccgcc aatcccgagc gcctgagtga gctgctgaaa
      781 ggggtgcggc gccttcggga acgccatgat ttcgtctggt tctgcgaagg gcacccgggt
      841 tttttagcgc gccgcccgga gctgatagcc gaaatgatcg cgagcggtat ggtccggatg
      901 cagatcggca tggagtccgg agatggtcgc gtccttcagg catacgggaa gcaggccgtt
      961 ccggaggacg tgaagaacgt ggtggatacc tgctaccggg aggggctgcc ccagctcgcc
     1021 gggaatttca tcatcggggg ggcttttgaa acccggcgca ccctcgaaac gaccaccggc
     1081 ttcgctcttg atctgctgca tcgagcgccg ggaatgctgg atctctccac cacttttctg
     1141 atgccgctgc cgggaacgcg aatttccaca catcccggga gcttcggcat cgcccttgaa
     1201 gaccgggact gtctgacgtc catcgaggac tttccggtga acagtacgct cgagctctcc
     1261 gttccggaaa tatgccgtga acggtcggtc tttctcacgc atatttccaa cgcgatgaag
     1321 gcgcagtacg cgaacggccg gattcctccg gagcggatca ggcgcgactt cgagctggcg
     1381 ttccggtacg gcatgcggcg gggtacctga agttcatcta cggcaaggac ccgcttctgg
     1441 tcgcctacta tcggaaactt accgactata agggacttct gaaagaatgg aacgaacttg
     1501 aaggagcgga aaagacaacc tccttcatcc agcgcgttcc tgatttttcg ctggtggatt
     1561 ttacagtctt gagcggcgcg gaaacggata tcctgctgga cgccggttcc tcttcggtga
     1621 aggagacggc gaaaaaactg aatctgtcca tcgaagcaac gagcgtctgc ctcgaaagac
     1681 tgagcgcgca gcatctgacg ctcttctcgc cctgcctgtg aaacggaatg cgatgagagc
     1741 gaacctgtcg tacacactac tctatggagg cccggagtga acactatcaa cctgagactg
     1801 atgctgattc atcgcggaac gagaacccat tcgatcaccc
//
LOCUS       DAOSWW010000588         1839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53848_length_1839_cov_2.463565, whole genome shotgun sequence.
ACCESSION   DAOSWW010000588 DAOSWW010000000
VERSION     DAOSWW010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53848_length_1839_cov_2.463565"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1091)
                     /gene="dctP"
                     /locus_tag="PK442_10820"
     CDS             complement(36..1091)
                     /gene="dctP"
                     /locus_tag="PK442_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO88187.1"
                     /translation="MKKRMMRCASLTLVLVFLFVFAGGAAAAPKYQWRLACETIAGSI
                     ADLYAQEFARLLKEKSNGDIQLDIFHSGTLGTPTEMFELTLNGAVEFCLTGPSQSSSI
                     VPENQIMGLQFLFSDDPIVNRDFFAQSKALNVMLNDIYREKGLSVLSYFTEGAMFWTA
                     NKAIRTPDDFKGVKIRVMPSELLVETYRAYGANPTPLSFTELYSGLQLNVVEGQENAP
                     ATIQEMKFMDVQKFLIGSRHNMYVMQHLVNVGFLDSLPEDVRKIVLDSVDEVRPYIHD
                     VQEELNKTRLQMMIDDFKSGQEYYDLTHEEREAFKEIAKKADAKYFELSRNPEFAKEL
                     LETFRAEMAELESKRGK"
     gene            complement(1255..>1839)
                     /locus_tag="PK442_10825"
     CDS             complement(1255..>1839)
                     /locus_tag="PK442_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88188.1"
                     /translation="GVFSPTEAAAVSVLYAFILEFFIYKSITLADIPKIALRTGVVTA
                     IVFILIAMGQVFSWTVSFARIPNLILPPLLGDAPSTFRILAVLSIAYFLGCMFVDPIV
                     VIMILTPIFKPAVVASGLDNVLVGTIVTLQAAIGSTTPPFGCNIFTAIAIFKQRYIDV
                     VRGVFPFIAMMVIVSILIIIFPQIALFLRDLMIQ"
BASE COUNT          423 a          501 c          511 g          404 t
ORIGIN      
        1 ggggggccga agcccccctc tgccgctttc agccgctact ttcctctttt gctctccagc
       61 tcggccattt cggcccggaa cgtttcaagg agctccttgg cgaactcggg atttctcgaa
      121 agctcgaagt acttcgcgtc ggctttcttc gcaatctcct tgaacgcttc tctctcctcg
      181 tgggtcaggt cgtagtactc ctgtcccgat ttgaagtcgt cgatcatcat ctgcaggcgg
      241 gtcttgttca gctcttcctg aacgtcgtga atatacgggc gtacctcgtc aaccgagtcg
      301 aggacgatct tgcgcacatc ctcgggaagg gaatcgagga atccgacgtt cacaagatgc
      361 tgcatgacgt acatgttatg gcgcgaaccg atgaggaact tctggacatc catgaacttc
      421 atctcctgga tggtcgcggg ggcgttctcc tgcccctcca ctacgttgag ctgaagtccg
      481 ctgtacagtt cggtgaagga gagcggcgtc gggttggctc cgtacgctct gtaggtttcc
      541 acgagcagtt cggagggcat aacgcggatc ttgacgccct tgaaatcgtc gggggtacgg
      601 atagccttgt tcgctgtcca gaacatggcg ccctcggtga agtaggagag aacggagagc
      661 cccttctccc ggtagatgtc gttgagcata acgttgagcg ccttgctctg agcgaagaag
      721 tctctgttca cgatgggatc gtcggagaag aggaactgaa gccccatgat ctgattctcc
      781 ggaacgatgg agctggactg gctcggcccg gtcagacaga attcgacggc gccgttgagc
      841 gtcagttcga acatctccgt cggcgtgccg agcgtgccgg agtggaagat gtcaagctga
      901 atatctccgt tcgatttctc tttcagcagg cgggcgaact cctgcgcata gaggtcggcg
      961 atgctccccg cgatcgtctc gcaggccaga cgccactgat acttgggagc ggccgcagct
     1021 cctccggcaa agacgaacag gaacaccagc acaagggtaa gactcgcaca acgcatcatt
     1081 ctctttttca tgatagaact cctccttttt ctttcgaaag tcatgtccga aacactctcc
     1141 ggaatctctt ctttatgccc cgaaacgccg tgaacttccc cctccttcct ctaaaaatcc
     1201 cctttatttt ttcaccccaa acacggttga atcccttaga gcgtaaccgt cgtcttattg
     1261 aatcatcaaa tccctgagga agagcgcgat ctgcgggaag atgatgataa ggatagagac
     1321 aatcaccatc atcgcgataa acggaaagac tcctcgtaca acgtcgatgt atcgctgctt
     1381 gaatatggcg attgcggtaa agatgttgca cccgaacgga ggcgtcgtgg aaccgatggc
     1441 cgcttggagg gtcacgatgg tgccgacgag cacgttatca agtccggaag cgacgacagc
     1501 aggcttaaaa atcggcgtta ggatcatgat cacgacgatg ggatcaacaa acatgcaccc
     1561 aaggaaatac gcaatgctga ggacggcgag tatcctgaaa gtggacggcg catcgcccaa
     1621 aagcggcggc aggatcagat tcgggattcg ggcgaaagag accgtccagg aaaagacctg
     1681 tcccatcgca ataagaatga agacgatggc ggtcacaacg cccgttctga gagcaatctt
     1741 cggaatatcg gcaagggtga tcgacttgta gataaagaac tccaggatga acgcgtagag
     1801 caccgagacc gcggccgctt cggtggggct gaacacgcc
//
LOCUS       DAOSWW010000589         1838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53870_length_1838_cov_3.776220, whole genome shotgun sequence.
ACCESSION   DAOSWW010000589 DAOSWW010000000
VERSION     DAOSWW010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53870_length_1838_cov_3.776220"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1282)
                     /locus_tag="PK442_10830"
     CDS             complement(104..1282)
                     /locus_tag="PK442_10830"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOO88189.1"
                     /translation="MLHDALAELPPGCAPACGACERPRSVEESLAGKKARAIRALSRW
                     EDRIEDIVSPPEERRLGYRDRTALKALWTPEEGWRFGMEGVRTDVRGPLRFARPRPFV
                     PLTDCPVHTERIRKMLHLPASALPPDEPEGSFPLSFLVVSGAQATFVFKAKTEPPSDG
                     WLFEPRDGSPSLADALGDIGIEGLWFHANPAAGVRLFAKRGWRLAWGAPRSADSRGLL
                     YGPASFSQLLPELHDASLDEARRFLVPGAGDAAADLYCGIGASLRLWRKCGADALGVE
                     GSGEAVECALHNAPGAAVLRGSCADRAPMISDFLRSVPPERRIAYVNPPRTGLEPQVT
                     ELLASHKLRGAAYLSCSPGTLARDLVILEKEYIVERLVPYDFFPRTAAVEVLALLRGR
                     "
     gene            1568..>1838
                     /locus_tag="PK442_10835"
     CDS             1568..>1838
                     /locus_tag="PK442_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88190.1"
                     /translation="MKVMIVDDAAFMRMMIRKHLEGAGHEVVAEAFEYHSAMDEFKKL
                     LKTGTLPDAITMDITLPGRRSGIDITREIHRLAPEVRIVMTSAMGT"
BASE COUNT          344 a          555 c          626 g          313 t
ORIGIN      
        1 attttgtcaa gagccctttt cgtaatcctt cggcgccgaa tatggagcaa caagatgcaa
       61 tgaacgcggg aaaaaaatgg aggatcggcg gtcccttgct gtgttatctc ccccgaagca
      121 gcgcgagcac ttcgacggcg gcggtgcgcg ggaagaagtc gtacggaacg agccgttcca
      181 cgatgtactc cttctccagt attaccagat cgcgcgccag cgtgcccggg ctgcacgaga
      241 ggtaggccgc tccccggagc ttgtgcgagg cgagtaactc cgttacctga ggttccagtc
      301 cggtgcgggg cgggttgacg tacgcgatgc ggcgttcggg cgggacggag cgcagaaagt
      361 ccgagatcat cggggcgcgg tcggcgcagc ttccccgcag caccgccgct cccggcgcgt
      421 tgtgaagggc gcactccacc gcttcgccgc tcccctccac gccgagcgcg tccgccccgc
      481 atttccgcca gaggcgcaaa cttgcgccga tgccgcagta gaggtcggct gcagcgtcac
      541 ccgcccccgg aacgaggaag cggcgcgctt cgtcgagcga cgcgtcgtgc agctccggca
      601 gaagctgtga gaaagacgcc ggtccgtaga gcagcccccg tgagtcggcg gagcggggcg
      661 cgccccatgc gagccgccac ccccgcttgg cgaagaggcg cactcccgcc gcaggattgg
      721 cgtggaacca gagcccttcg attccgatgt cgccgagcgc gtcggcaagg gaggggctgc
      781 cgtcgcgcgg ctcgaagagc cagccgtccg aaggcggctc cgtcttcgcc ttgaagacga
      841 acgtcgcctg cgccccggag acgacgagaa agctgagtgg gaagctcccc tccggttcgt
      901 cgggcgggag ggcggacgcc ggcaggtgca gcattttccg gattcgttcg gtatgcaccg
      961 ggcagtcggt gagcgggacg aaggggcggg gacgcgcgaa gcggagcggg ccgcgaacgt
     1021 ccgtccggac gccctccatg ccgaaacgcc acccttcttc aggcgtccaa agcgccttca
     1081 gggcggttcg gtcacggtac cccaggcgtc gttcctccgg gggcgacacg atgtcctcga
     1141 tgcgatcctc ccagcgggag agggcgcgga tggcgcgggc cttcttcccg gcgagcgact
     1201 cctcgacgga gcgcggccgt tcgcacgcgc cgcaggcggg ggcgcacccg ggaggaagtt
     1261 cggcaagagc gtcatggagc acgtatggtc acctcgtctt cgtgtgttct cgctcaagca
     1321 taccgtacgc gctgcaagcg cgccagactg caatcgaaca gtacggagaa agagagtgtg
     1381 cgtactttcg tcgccgcgat ctctcggaat tgccggaccc tcggcgtcct ccgtatcgaa
     1441 gcggcgctgt cgaatcgatt ttcgggggtg cgaatttcct tcgaagaggc tatactatgt
     1501 taactgggta ttgttttttt gcttcgatga aagcatgaga atgactcgtt cgacggaggg
     1561 tacgggaatg aaagtgatga tcgtggacga cgccgcattc atgcgcatga tgatacgaaa
     1621 acacctggag ggagcggggc acgaagtcgt cgcggaggcg ttcgaatacc actcggcgat
     1681 ggacgagttc aaaaaactgc tgaagaccgg tacgcttccc gatgcgatca cgatggacat
     1741 tacgcttccc ggcagacggt cggggataga tattacgcgc gaaattcaca ggttggctcc
     1801 ggaggtcagg atcgtgatga cgtccgcaat gggaacgc
//
LOCUS       DAOSWW010000590         1838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_53885_length_1838_cov_2.641615, whole genome shotgun sequence.
ACCESSION   DAOSWW010000590 DAOSWW010000000
VERSION     DAOSWW010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53885_length_1838_cov_2.641615"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PK442_10840"
     CDS             complement(<1..756)
                     /locus_tag="PK442_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO88191.1"
                     /translation="MGGLVRFCIKRPVFTWAMVLVLMLLGVSSYNKLAVALLPKVDIP
                     IVVVRTVYRGASPNEIEQLVSKPIEDAIADLEGLDKITSYSAEGMSFVVLEMEVQVDV
                     DQALVDISNKVKAARNKLPDGVDEPIFSKVDINASPFMIVSFTSTLAEKDARKIIEDT
                     VVPILSRVDGVGQTDVTGGRNRQIRILLDPVSLSEYGVSIQTLAAVVASNNVTNPSGY
                     ITQTKDETWLRLVGEFNKVDELENIMIPTPSGNP"
     gene            complement(759..>1838)
                     /locus_tag="PK442_10845"
     CDS             complement(759..>1838)
                     /locus_tag="PK442_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO88192.1"
                     /translation="VVLLCAGGVLMVLRVQARLEESAANAAAMEQQEETAVPVDVQKL
                     VRRDWEVWRSYYGQAKAGRSQTVSSYVKEIVRDVHVRIGDKVKAGQVLLTLSSQDQRA
                     TEAALRSSYEDSVREYQRLSELQKSGGVSKSQVEQALSRMKSEEARLQASRSTLSRTQ
                     LRASIDGIVASRMIEPGEVAGENKPLLTVIDLKDLEAEIMVSRRDIMSLSTETPVEVV
                     SDGARAMGVIRRISPEAAPGSGLYTVVVGLNGLDILPGTHVEARFLVERRENLLVVPS
                     DIVQRRGDKAYVYVVEGDRAKLREIVPGEGQNGSLAVAEGLAEGELLVIRGQNLLSDG
                     ALTRISGTGAPEAASSDIAEKTAEK"
BASE COUNT          336 a          612 c          523 g          367 t
ORIGIN      
        1 aggattcccg gacggtgtcg gaatcatgat gttctcgagc tcgtccacct tgttgaactc
       61 gccgacgagg cgaagccagg tctcgtcctt cgtctgcgtg atatacccgg aggggttcgt
      121 gacgttgttc gacgcgacga ccgccgcaag cgtctggatg ctcaccccgt actcgctcag
      181 cgaaacggga tcaagcaata tccgtatctg ccggttccgg cctccggtga catcggtctg
      241 tcccacgccg tccacgcggg agagaatagg gacgaccgta tcctcgatga tcttccgcgc
      301 atccttctcc gccagcgtgc tcgtgaagga gacgatcatg aaggggctcg cgttgatatc
      361 gaccttgctg aagatgggct cgtcgacccc gtcgggcagc ttgttccggg ccgccttcac
      421 cttgttggag atgtccacca gggcctggtc gacgtccacc tgaacctcca tctcaaggac
      481 gacgaaggac atgccctcgg cagagtagct cgtgatctta tcgagcccct caaggtcggc
      541 gatggcatcc tcgatcggct tggagacgag ctgctctatt tcattgggac tggctcctct
      601 gtagactgtc cgaaccacca cgatggggat gtccaccttg ggcagcagcg caaccgccag
      661 cttgttgtag ctgctcaccc ccagcagcat gagaacgagc accatcgccc aggtaaacac
      721 cgggcgcttt atgcagaaac gtacaagacc gcccatgatt acttttcagc cgtcttttcc
      781 gcaatatcgg atgacgctgc ttcgggcgct cccgttccgg agatccgagt caaggcgccg
      841 tcagaaagca ggttctgtcc tctgatcacc agaagctctc cctcggcaag cccctcggcc
      901 actgcaagag atccgttctg cccctcgccc ggcacgatct cgcgaagttt cgccctgtcg
      961 ccctcgacga cgtagacgta cgccttgtcg ccgcgccgct ggacgatatc cgaagggacg
     1021 acaagcaggt tttcccgccg ttccacgagg aatcgagcct cgacgtgcgt tcctggaagt
     1081 atgtcaagcc cgttgaggcc gacgacgacc gtgtacagtc ccgaccccgg cgccgcctcc
     1141 ggactgatcc tccggataac gcccattgcg cgggcgccgt cggagacgac ctctaccgga
     1201 gtctccgtgc tgagactcat gatgtccctt ctggagacca tgatctccgc ctcaagatcc
     1261 ttcagatcga tgacggtgag caagggtttg ttctcgcccg ccacctctcc cggttcgatc
     1321 atgcgcgagg cgacgatacc gtcgatactc gccctgagct gcgtgcgcga gagggtggag
     1381 cgcgacgcct gaagtctcgc ctcctcgctc ttcatgcggg agagcgcctg ctccacctgc
     1441 gacttggaga cgccgcccga tttctgaagc tcggacagac gctggtactc ccttacggaa
     1501 tcctcgtaac tggaacggag cgcagcctcg gtggcgcgct ggtcctgcga ggagagcgtc
     1561 aggagcacct gtcccgcctt caccttgtcg ccgatgcgca catgaacatc acggacgatc
     1621 tccttgacgt aggaagaaac cgtctggctg cgcccggcct tcgcctggcc gtagtagctc
     1681 cgccagacct cccagtcgcg tcggacgagc ttctgcacat cgaccggaac ggccgtctcc
     1741 tcctgctgtt ccatagctgc ggcgttggcc gcggactctt caagcctcgc ctgaacgcgg
     1801 agcaccatca ggacgccccc ggcgcaaaga agtacgac
//
LOCUS       DAOSWW010000591         1834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_54060_length_1834_cov_3.211917, whole genome shotgun sequence.
ACCESSION   DAOSWW010000591 DAOSWW010000000
VERSION     DAOSWW010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54060_length_1834_cov_3.211917"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PK442_10850"
     CDS             complement(<1..889)
                     /locus_tag="PK442_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOO88193.1"
                     /translation="MLQQRRSHHWNPALCRAFVLDWDGVLAETKLNFSPIREKFFQGR
                     FVPLFEAIATLPQPLAEELSKDIYDVEMTGAADAKPVPGALELLNWLESNNIPWCVVS
                     RNCADSILLAAQRANLPLPPLLFSRDTPPVKPAPEALWRAAEALDVPAADCVMIGDFL
                     YDLVGARRAGMRALLVQRPQAEWKHWADATFDTLTEFVEYLRAPTPLVPWEYHHIASQ
                     EGGETALELLGKYVFSISSSSIDILPLCLEQASKGVLNFFIEGEGNLTTEQWFNLAGL
                     SPVWLDQPVKEVLDHLLHAR"
     gene            complement(1211..>1834)
                     /locus_tag="PK442_10855"
     CDS             complement(1211..>1834)
                     /locus_tag="PK442_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOO88194.1"
                     /translation="VFSVGLMMVVISGSELFTGNCMMPLGYLAGCTPLPKILRNWGWV
                     YVSNLVGSILIAALIYYSGLADNAVGGRALQVASGKMGLSFGQAFFRGVLCNWIVVLA
                     VWMAMAATDIVGKVWAIFFPIMTFVAAGFEHSIANMYFMALGIFLKGTPAAVTASGLS
                     EQALANVGLGGYMSNLIPVTLGNMVGGILFVAVFYYFIFKDGLTDID"
BASE COUNT          465 a          500 c          492 g          377 t
ORIGIN      
        1 atcgggcgtg cagcaaatgg tccagaacct cctttaccgg ctgatccaac catacgggag
       61 agagacctgc gagattgaac cactgttctg ttgtgagatt gccttctccc tcgatgaaaa
      121 agttgagcac gccttttgat gcctgctcca gacagagagg caaaatatcg atgcttgagc
      181 tggatatcga gaatacatat ttcccgagca attcaagtgc cgtctccccg ccttcttgag
      241 aggcgatatg gtggtattcc caagggacaa gaggagtcgg cgcacggaga tactctacaa
      301 attcggtcaa tgtatcgaaa gtggcgtccg cccagtgctt ccactctgct tgcggacgct
      361 gcacaagcag agctctcatc cctgcgcgcc tcgcgccgac aagatcatag aggaaatccc
      421 ctatcatgac acaatcggcg gcggggacat caagcgcttc cgctgcgcgc cacaatgctt
      481 cgggagctgg ttttacaggt ggggtgtccc tgctgaaaag caaaggaggg aggggaaggt
      541 tggctcgttg tgcagcaaga agaatggagt cggcacaatt ccgtgaaacg acgcaccagg
      601 ggatattgtt gctctctaac caattcagga gttccaatgc cccggggaca ggtttcgcgt
      661 ctgcggctcc ggtcatttcc acgtcgtaaa tgtctttgct caattcttca gcgagcggct
      721 gtggcaacgt agcgatagct tcaaaaagag gtacgaagcg cccctggaaa aacttctctc
      781 ggatcggtga aaagttcaat ttcgtttcag caagcacacc atcccagtca agcacaaatg
      841 cacggcataa tgcgggattc caatggtgtg agcggcgttg ttggagcact ggatacccct
      901 cctcatataa aagacagggg cgcgggcttc cccgtcccct gtagccaagc ttccccgcaa
      961 tgtcgtgtca tgaacgtctt gacccgttcc tatagtattg gggcctgctg aaccttagtt
     1021 tcgtcatcgc acgaggcaat tcctctaccg gcccaaactg gcggccccgc aaggcgcgag
     1081 tgttggctca agacaaaaat catgatcacg ggcaccgcag ggactctttc tttcttgttg
     1141 tatattatag cacaaaaatg ggaatcgccc agtcgcaggc gattcccaaa agagagaaaa
     1201 gataaaatga ctaatcgatg tctgtgagac cgtccttgaa gatgaaatag tagaacacgg
     1261 cgacaaagag aattccgccg accatattcc ccagcgtaac gggaataagg ttgctcatgt
     1321 agccgcccag gccgacgttg gcaagcgcct gctcggaaag gccggatgcg gttacggctg
     1381 ccggcgttcc cttcaggaag atcccgagag ccataaagta catgttcgcg atggagtgtt
     1441 caaaaccggc cgcgacgaat gtcatgatcg ggaagaagat tgcccatact ttcccgacga
     1501 tatctgtcgc cgccatcgcc atccagacgg cgagcacaac gatccagttg cagaggacgc
     1561 cgcggaagaa tgcctgtccg aaggagagtc ccatcttacc ggaagctacc tggagcgccc
     1621 ttccgccgac cgcattgtca gccagcccgg agtaatagat aagcgcagcg ataaggatgc
     1681 tgccgacaag gttcgaaacg tagacccaac cccaattccg aagaatcttc ggcagaggcg
     1741 tacagcccgc aaggtatccc agaggcatca tgcagtttcc cgtaaagagc tcggagccgc
     1801 tgataacaac catcatgagt cccacgctga agac
//
LOCUS       DAOSWW010000592         1830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_54246_length_1830_cov_3.986479, whole genome shotgun sequence.
ACCESSION   DAOSWW010000592 DAOSWW010000000
VERSION     DAOSWW010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54246_length_1830_cov_3.986479"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK442_10860"
     CDS             <1..388
                     /locus_tag="PK442_10860"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556676.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HOO88195.1"
                     /translation="QGNHLGVTIGADIIKQKLPENNGGYTALKFGKTHKNVYDKLSSD
                     HPIDLTRYQVANCYMGRAGLINSGGASGGESDMAEAVKTAVINKRAGGMGLILGRKAF
                     QRPMNEGVEIIHSVQDVYLEEQVTIA"
     gene            complement(336..>1830)
                     /locus_tag="PK442_10865"
     CDS             complement(336..>1830)
                     /locus_tag="PK442_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOO88196.1"
                     /translation="LYERVRKEALDLGCVGVFFECLPDDPALSRNDTVRKQNVARLRF
                     YERYGATPLVGTKYETPLEPEGDNPPYLLFDGLGKKEPLRREKARLVVAAILGKKYGA
                     LCSPEYIRMVMDSFRDDPVKLRPLRYVGAPTRVAVASELPESERIALFVNRDHAIHHV
                     RERGYVESPVRVEAILKEIVPSGLFSEKKPRHFGEKHILKVHGAVFFHYLRKVCLSMP
                     EGESVYPYVFPVRNAARPPQDLSVRAGYFCMDTFTPLNKNAFLAALGAVDCALSGAQT
                     LVEGRHLAYALVRPPGHHAERRLFGGFCYLNSGAAAAEYLSAFGKVAMLDLDYHHGNG
                     QQDIFYARKDVLTISLHGHPRFAYPYFSGFEDERGEGEGRGYSLNIPLPEKMSGSDYV
                     DPLRRAIRRIEDFAPSFLVVALGGDTCKNDPTGTWSLAAKDFEINGRLLGALDLPVLV
                     IQEGGYNTRLLGENIRSFFKGLFQSSRSAGARLWSPAPRDRRPVRNG"
BASE COUNT          364 a          580 c          587 g          299 t
ORIGIN      
        1 acaggggaac cacctcggcg tgaccatcgg cgccgacatc atcaagcaga agctcccgga
       61 gaacaacggc ggctacaccg cgctgaagtt cggcaaaacg cacaagaacg tctacgacaa
      121 gctctccagc gaccacccca tcgacctcac ccgctaccag gtcgccaact gctacatggg
      181 ccgggcggga ctgatcaact caggcggcgc atcgggcggc gaaagcgaca tggccgaggc
      241 cgtaaagacc gccgtgatca acaagagggc cggcggcatg gggctgatcc tcggacggaa
      301 agccttccag cgccccatga acgaaggagt ggagatcatc cattccgtac aggacgtcta
      361 tctcgaggag caggtgacca tagcctagcg cccgccgaac gggacgactg aaagagcccc
      421 ttgaagaagg agcggatgtt ttctccgaga aggcgggtat tgtacccgcc ttcttgtatg
      481 acgagcacag gcaggtcgag cgcgccgaga agacggccgt tgatctcgaa gtccttcgcg
      541 gcgagactcc acgtccccgt agggtcgttc ttgcaggtgt cccctccgag cgcgacgacg
      601 agaaagctcg gcgcgaaatc ctctatccgc cggatggcgc gccgcagcgg gtcgacatag
      661 tccgaaccgg acatcttctc cggcagcggg atgttgagcg aataccctct cccctccccc
      721 tctccccttt catcctcgaa ccccgagaag taggggtagg cgaagcgggg gtgcccgtgg
      781 agcgagatag tgagcacatc cttccgcgcg tagaagatat cctgctgccc gttgccgtga
      841 tgatagtcga ggtcgagcat ggcgaccttg ccgaacgcgg agaggtattc ggccgcagcc
      901 gcgccgctgt tcaggtagca gaatcctccg aagagccttc gctcggcgtg gtgtccgggc
      961 ggacggacca gcgcatacgc cagatgccgc ccctcgacga gcgtctgcgc gcccgagagc
     1021 gcgcagtcca ccgcgcccag agccgcgaga aaggcgtttt tgttcagcgg ggtgaaggtg
     1081 tccatgcaga agtatccggc ccgcaccgag agatcctgcg gcggccgggc ggcgttccgt
     1141 accgggaaga cgtacgggta gacggactcc ccctccggca tggagaggca gacctttcgc
     1201 agatagtgga aaaaaaccgc gccgtggacc ttcaagatgt gcttttcgcc gaagtgacgg
     1261 ggcttcttct cggagaagag cccggacggg acgatctcct tgaggatggc ctccacgcgg
     1321 accggcgatt cgacgtatcc acgctcgcgc acgtggtgaa tcgcatggtc gcggttcacg
     1381 aagagggcga tgcgctcgct ttccgggagt tccgaggcga ccgctacgcg cgtcggcgca
     1441 ccgacgtagc gcagcggacg cagcttcacg gggtcgtccc tgaaggaatc catcaccatg
     1501 cggatatact cgggcgaaca gagagcgccg tacttcttgc caaggatcgc cgcgacgaca
     1561 agccgcgctt tctccctgcg gagcggctct tttttcccga ggccgtcgaa gaggaggtac
     1621 ggcggattgt cgccctcggg ctccagcggg gtctcgtact tcgtcccgac gagcggcgtc
     1681 gccccgtacc gctcgtagaa gcgcaggcgg gcgacgttct gctttcgcac ggtatcgttc
     1741 cggctcagcg ccgggtcgtc ggggaggcat tcaaagaaaa caccgacgca tccaaggtcg
     1801 agcgcctcct tccggaccct ctcgtagagg
//
LOCUS       DAOSWW010000593         1830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_54252_length_1830_cov_3.504789, whole genome shotgun sequence.
ACCESSION   DAOSWW010000593 DAOSWW010000000
VERSION     DAOSWW010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54252_length_1830_cov_3.504789"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK442_10870"
     CDS             <1..278
                     /locus_tag="PK442_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO88197.1"
                     /translation="AEILRKEGKEPSEAILEACKVRLRPIIMADATSIIAMIPLAMGV
                     GAGGAFRSPMAVVSIGGLVAGGVLALFVIPPVYKLVWKAKYRLGFME"
     gene            335..1639
                     /locus_tag="PK442_10875"
     CDS             335..1639
                     /locus_tag="PK442_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM family putative metalloenzyme
                     maturase"
                     /protein_id="HOO88198.1"
                     /translation="MNSALPPIERPYPSKLFVEVTTACNLRCAMCVKQSGPGIPDEHM
                     TMSTFQRLVPAFPMLETLLLNGIGEPLLHPRLEAFVQLAKEHLQEKARVGFQTNGTLL
                     TPERARSLLAAGTNIVCLSVDATDPALFQSMREGGETEDVNRAAAALNAARARASSPD
                     FHWGAEIVLTRDTLDELPRVVEWVGERGGAFLLVTHLLPYRQESVPSVAYNPNVDETL
                     AFYREKSREANEQGIDLSRYIEARWRFRPVDNGERTVAFMEKVITEISARGLPQHVPN
                     LVAHDEKQYRRMEELFRRCEELALQHGVELHLPSLSPRNKRRCDFIEEGSAFVAASGT
                     VHPCYFLWHSFTCHADGRIRPVDALSFGSVNETPLLDIWNGPEFSDYRSEIGRYDFPH
                     CGNCSLAPCDYIERHDFEQDCLGNSLTCGSCPWSVGVLQCLR"
BASE COUNT          375 a          538 c          545 g          372 t
ORIGIN      
        1 acgcggagat tctcaggaag gaagggaaag agccctccga ggcaatcctg gaagcctgca
       61 aggtgcgtct tcgcccgata atcatggcgg acgcaacatc aataattgcc atgattccgc
      121 tggcgatggg agttggggcg ggcggcgcct tccgctctcc catggcggtc gtttccatcg
      181 gcggacttgt cgcaggcggc gttctcgcgc tttttgtcat tccgccggtc tataagctcg
      241 tctggaaggc aaaataccgt ctcggtttca tggagtaaag aagtatatac aacggacttt
      301 cgcagcgtta ggaacaattt ggaggttttg gtacatgaac agcgcgcttc cccctattga
      361 acgtccatac ccctcgaagc ttttcgtcga ggtgaccacc gcatgcaacc ttcgttgcgc
      421 gatgtgcgtc aagcagtccg gccccggaat accggacgag cacatgacca tgagtacgtt
      481 tcaacgcctc gtcccggcat tccctatgct ggagacatta ctgctcaacg gcatcggcga
      541 gcccctgctc cacccgaggc tcgaggcttt cgtccaactg gcgaaggaac atctacagga
      601 gaaagctcgc gtcggttttc agacgaacgg cacgctgctc actccggagc gtgcgcgctc
      661 gctccttgcc gcaggaacca acatcgtctg tctctccgtg gacgcgacgg acccggcgct
      721 ctttcagagc atgcgggaag gaggagaaac cgaggatgtg aatcgtgccg ccgcggccct
      781 gaacgcggcg cgtgcacgcg cctcctcccc ggatttccac tggggggctg aaatcgtgct
      841 cacccgggac acactggacg agctgccccg ggtggtcgag tgggtagggg agcgaggggg
      901 agcgttcctt ctggtaacgc accttcttcc atacaggcag gagtcggtac caagcgttgc
      961 gtacaacccc aacgtggatg aaacgcttgc tttctacaga gagaagagca gagaggcaaa
     1021 tgaacagggg attgacctct cccgttacat cgaggccaga tggagattcc gtcccgtgga
     1081 caatggtgaa agaacggttg ctttcatgga gaaggtaatc actgaaatat ctgcgcgcgg
     1141 actgccccag cacgtcccca acctcgtcgc gcacgacgaa aagcagtatc ggcggatgga
     1201 ggagcttttc cgccgatgtg aggagcttgc gttgcagcac ggagtggaac ttcatctccc
     1261 ctcgcttagc ccgcgcaata aacggcggtg cgacttcatc gaggagggga gcgcgtttgt
     1321 cgccgcctcc gggacggtgc acccctgcta cttcctgtgg cactccttca cgtgtcatgc
     1381 ggacggtcga atcaggcccg tcgacgcgct ctccttcgga tcggtgaacg aaacgccgct
     1441 cttggatata tggaacggcc cggagttctc cgactaccgg agcgagatcg gacggtacga
     1501 cttcccccat tgcggcaact gcagcctcgc gccgtgcgac tatatcgagc gccacgattt
     1561 cgagcaggat tgcctgggga actcccttac ctgcggatcg tgcccatgga gcgtcggcgt
     1621 gcttcagtgt ctccggtaac ctttttctct ccgcacttta cagggctccg tccgggccgg
     1681 agaactctcc ggagggcgga gccttgtgaa ttctccggcg cacgagcttg acaatgcccg
     1741 gaatttcaaa gaagacgctc ccggcgagca gtatgtacgg aatgccgagg atgagggacg
     1801 tccatatcat cagtacaggc cctttgaata
//
LOCUS       DAOSWW010000594         1824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_54563_length_1824_cov_3.083663, whole genome shotgun sequence.
ACCESSION   DAOSWW010000594 DAOSWW010000000
VERSION     DAOSWW010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54563_length_1824_cov_3.083663"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1440
                     /locus_tag="PK442_10880"
     CDS             190..1440
                     /locus_tag="PK442_10880"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HOO88199.1"
                     /translation="MKFAVVPVLCLLLLCGAAWGGEHPLDVEIQECMDKDPSTHGTIK
                     CAREGAKKWDTELNRAYKELMGLLPKQGQDALRTAQRAWIPWRDREFGLVGSVYGAIY
                     NSLDGGTMWLVVNAIAEMEVVRGRTLELIAWSEELKKRKPVFEGAYPEKQTDEQLAAA
                     MRVKNASLRLGKRIGKEGEKAAAANLAAWEEFRNRNVLFMASFYGKQREKSFPLHARM
                     LMNNDRVKRLEGLLEDLNQNGLEESSGQEETGQKDALPKEPFQGDRSLYLPAEGQCDF
                     KAYIIDPDPEGANVRDAPGGKIIATLPHRPDDPALITVSVTGHKNKWLSVLLHDGRKG
                     WVFGELVGVSLRNYAPGDVAALRTKPSSDAPATGDIFGDEEVSIIGGEGKWALVQYRH
                     PKGHVLTGWLDPVKQCANPYSTCP"
     gene            1431..1661
                     /locus_tag="PK442_10885"
     CDS             1431..1661
                     /locus_tag="PK442_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88200.1"
                     /translation="MPMNHRTIQRCCIVAGVGLLLWTSVEFFLLAFLTTRFMEGAGIL
                     LWLQGYLMALRSSPVGIAGALLVLFAFFMRRM"
BASE COUNT          419 a          436 c          592 g          377 t
ORIGIN      
        1 cttggaatgg acgcggaaga agcggcttca cggctcggta cgcctattct gcggcaggaa
       61 gatacgctga ttttcggctt cgagcgcgac gagatagcgc tcacggaggg aaagtcgggc
      121 ggcaggacgg tcgtcgccgg gttggcctac cgccgggttg tctatgattg aagatggagg
      181 tggggtttca tgaagttcgc ggttgtgccg gtgctctgcc tgcttctgct gtgcggcgcc
      241 gcgtgggggg gcgaacaccc tctggatgtt gagatccagg agtgtatgga caaggatccg
      301 agcacccacg gaacgatcaa gtgcgcccgg gaaggggcga agaagtggga tacggagctc
      361 aacagagcat ataaagagct gatggggctg cttccaaaac agggacagga tgccctgcga
      421 acggcccagc gggcctggat cccctggaga gacagggagt tcggactggt gggttcggtg
      481 tacggagcga tctacaacag tctcgacggt ggcacgatgt ggcttgtcgt caacgccatc
      541 gcggagatgg aggtcgtcag gggaagaacg ctcgaactca tcgcatggtc ggaggaactg
      601 aaaaaaagga agccggtttt cgagggcgcg tatccggaaa aacagacgga tgaacagctt
      661 gccgccgcga tgagggtgaa gaacgcaagt ctccgcctgg gaaaacgaat cgggaaagag
      721 ggagagaagg ctgctgcggc aaaccttgcg gcgtgggagg agttcaggaa caggaatgtg
      781 ctcttcatgg cctccttcta cgggaagcaa agggaaaaaa gtttccccct gcatgcgcgc
      841 atgctgatga acaatgaccg ggtgaagcgt ctcgaaggat tgctcgaaga tctgaaccag
      901 aatgggttgg aggaatcgtc aggacaggag gagaccggac aaaaggatgc gcttcccaaa
      961 gaacctttcc aaggagatag atcgctctat ctgccggcag agggacaatg cgacttcaaa
     1021 gcgtatatca tcgaccctga tcccgagggg gccaacgtac gcgacgctcc cggaggaaag
     1081 atcatcgcca cgctgcctca ccggcctgac gatcccgctc tcattacggt cagcgttacg
     1141 gggcataaga acaagtggct ctcagttctc ctccacgacg gaagaaaagg atgggtattc
     1201 ggcgaactgg tgggcgtgag ccttcgaaac tatgctcccg gcgatgtggc ggcgctccgc
     1261 accaaaccat cctctgatgc cccggcgacg ggagatatct tcggagacga ggaggtctcg
     1321 atcattggcg gagaaggcaa atgggcgctg gtgcagtaca ggcatccgaa ggggcacgtg
     1381 ctcacagggt ggcttgatcc cgtgaagcag tgcgcgaatc cgtattcgac atgcccatga
     1441 atcaccggac gatccaaaga tgctgcatcg tcgctggagt ggggctgctt ctctggacgt
     1501 ccgtagagtt ttttcttttg gcattcctga caacacgttt tatggagggc gcaggaatcc
     1561 tgctctggtt gcagggatac ttgatggcgc tccgctcttc gcctgtgggg atcgcagggg
     1621 cgctgctcgt tctttttgcc ttcttcatga gaagaatgta aaaaatgctc attacgtttc
     1681 ttaagcggtt agttgctgca cttcaggtca aataaatctt gacaaggacg ggcgtttacg
     1741 ggatagtatt cgaaagttct tgtgctggtg tttttgtaga aacgcacctt gtatactagt
     1801 attctttagg aggccgcatg ctgt
//
LOCUS       DAOSWW010000595         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_54794_length_1820_cov_1.692918, whole genome shotgun sequence.
ACCESSION   DAOSWW010000595 DAOSWW010000000
VERSION     DAOSWW010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54794_length_1820_cov_1.692918"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1027
                     /locus_tag="PK442_10890"
     CDS             44..1027
                     /locus_tag="PK442_10890"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO88201.1"
                     /translation="MRVRFTMLLVSALVLFLFPAAAFPAEYEINLGHTASISHHYHTS
                     SELFAKLVAEKTGGKVNIVIFPAGQLGSLPDMTESVRLGTQDIVLTADPILGNTIGEF
                     EALYLPYIFRDYDHVEKFEESNAAKMLEDILRENGMVLLGWWENGFRVITNNKRPIKH
                     PDDMAGLKLRIGESKMAIDTFVLLQANPTPIAMSELFAALQLGTVDGQENPTGRILSA
                     RYYEVQKYLSVTRHQHVFEPLVMNLDKFKTLPAEFQQALLDAGKEVAKADRKAVADTE
                     ADELAQLEQKGMLVNEVDVPAFQKALSPMYEQYMKERGEKWSEIVKMIQGL"
     gene            1102..1593
                     /locus_tag="PK442_10895"
     CDS             1102..1593
                     /locus_tag="PK442_10895"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO88202.1"
                     /translation="MRIDALVGVLNSIVKYILVALVLAMASILLLQIISRFVVFFPLP
                     WSQELLQYMNIWMVFLGAGLAVKEGGHIRVALLRERFPKGFKRPFDIFSNALSFSLVV
                     FVAVQAYTLVGRTAKQSFGSFPLAMSWFYLSLLLGCSLMAINYILIIVHAVSGMRKER
                     SRP"
     gene            1590..>1820
                     /locus_tag="PK442_10900"
     CDS             1590..>1820
                     /locus_tag="PK442_10900"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88203.1"
                     /translation="MSGFIFTAFFVLLLSSVPIALSLAGSALLGICFKTSVPPFIVAQ
                     RMVGGINGFPILAIVFFILAGNIMTEGGVSKRL"
BASE COUNT          381 a          449 c          523 g          467 t
ORIGIN      
        1 gaagcggttc ttcgaataaa tcatatttac tggaggtgtt gttatgagag tccgcttcac
       61 aatgctgctt gtttccgcgc ttgtactgtt tctttttcct gccgccgctt ttccggctga
      121 atacgagatc aatctggggc acacggcgtc cataagccat cactatcaca catcctcgga
      181 gctttttgcg aaacttgtgg ctgaaaaaac aggaggcaag gtgaatatcg tcatctttcc
      241 ggcgggacag ctcggttcac tgcccgatat gacggaatcg gtccgtcttg gtacgcagga
      301 tatcgtcctg acggcggatc cgattctcgg gaatactatc ggcgagttcg aagcgttgta
      361 ccttccctat atcttccgcg actacgacca tgtggagaag ttcgaggaga gcaacgcggc
      421 caagatgctg gaggatatcc ttcgagagaa cggaatggtg cttctggggt ggtgggagaa
      481 cggcttccgg gtgatcacga acaacaagag gcccatcaaa caccccgacg acatggctgg
      541 attgaagctg cgtattggag agtccaaaat ggctatcgac acgttcgtcc tgctgcaagc
      601 caacccaacg cccatcgcca tgagcgagct tttcgcagcg cttcagcttg gaacggtcga
      661 cggccaggag aatccgacgg gaagaatact ctcggcgcgc tactacgagg tgcagaagta
      721 tctttccgtg acgcggcatc agcatgtctt cgaaccgctg gtgatgaatc tggacaagtt
      781 caaaacgctt cccgccgaat ttcagcaggc actccttgat gcaggaaaag aggtggccaa
      841 ggcggaccgg aaggccgtgg ccgacacgga ggccgacgag ctggcgcagc tggagcaaaa
      901 ggggatgctg gtaaacgagg tcgatgtgcc cgctttccaa aaagcgctca gtcccatgta
      961 tgaacagtac atgaaggagc gcggagagaa gtggagcgag atcgtgaaga tgatacaggg
     1021 gttgtagatg cataggtttg aaagtgaatg gcggccggtc tgtgctccgg ccgctttttc
     1081 ttcgccggaa aggatgattg tatgcgtatc gacgctctcg tgggtgtgct taatagcatt
     1141 gtaaaatata ttctggtagc gcttgtgctc gccatggcat ctattttgct cttgcagatc
     1201 atctcgcgtt tcgtggtctt tttccctctt ccgtggtcgc aggagctgct tcagtacatg
     1261 aacatctgga tggtctttct cggagctggg ctcgcagtca aggagggggg gcacataagg
     1321 gtggcgctgc ttcgtgagcg ctttccgaaa ggattcaagc gcccctttga cattttctcc
     1381 aatgcattga gtttcagtct tgttgttttc gtcgcggttc aggcatatac gctcgtgggc
     1441 agaacggcga aacagagttt cggctccttc cctctcgcga tgtcctggtt ttatctcagc
     1501 ctgctgctgg ggtgctcgct catggccatc aactatatcc tgatcattgt acatgcagtc
     1561 tccgggatga ggaaagaaag gagtcggcca tgagcggatt cattttcacc gcatttttcg
     1621 ttctcttgtt gtcttccgtg cccatagccc tctccttggc gggttcggct ctccttggga
     1681 tttgtttcaa gacgtccgtc ccgccgttta ttgtcgcgca gcggatggtc ggtggtatta
     1741 acggattccc gattctcgcg atagtgtttt ttatccttgc ggggaatatc atgacggaag
     1801 gcggcgtttc aaagcgcctg
//
LOCUS       DAOSWW010000596         1819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_54824_length_1819_cov_2.626984, whole genome shotgun sequence.
ACCESSION   DAOSWW010000596 DAOSWW010000000
VERSION     DAOSWW010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54824_length_1819_cov_2.626984"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1191)
                     /locus_tag="PK442_10905"
     CDS             complement(151..1191)
                     /locus_tag="PK442_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO88204.1"
                     /translation="MKNIISPGDSVLLKANMLSASTPEECITTHPEVVRAVAVQTLEA
                     GGKPFIADSPGIDRFSDVARKTGMEAVARELGIPCVELDDPVPLPVAEGNVFQRIEVS
                     RRAVEADTIINIPKLKTHAQMMLTLGVKNLFGTVVSQRKAQWHYNVGLRREAFADLHL
                     DIARGLAPRLTILDGIRGMEGRGPSNGKPRDFGILAASSDPLDLDFRVCTLLGAPLEE
                     FPLYRAARERGLVDKSARWDAPGLPEEFEIPPIELPKLDALHLLPPFLDRFGKRFLAS
                     RPVQEKQKCIRCGKCVEICPAGAMELTKAGDIEIDYEKCIRCYCCQEVCPADAIRFSK
                     GLLLRVLEGLRR"
     gene            1373..>1819
                     /locus_tag="PK442_10910"
     CDS             1373..>1819
                     /locus_tag="PK442_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88205.1"
                     /translation="MAYNVQQFFLQKRGVGYVAPILCTMRGLGESDIEQAVVFHKRIS
                     DEIGDADFFAEENTIAESIMGEGVVVGVFAEGKLIALRAVSYVKEFVDDAVVDLDLDP
                     GEADHVAVMDFCVTDSSFRGNSIQFFTYLFTESQMYPNRYHLHTTVS"
BASE COUNT          362 a          546 c          536 g          375 t
ORIGIN      
        1 cgcgcaggag gaaaacagaa aaaacgcggc gccgagcaga agagccagga gcgagccggc
       61 cagcattttt ttcatgacgt catctccttt cctcggttgg atgaagcatg gcgccattat
      121 accccaacga aacgatgggt agcgtgctca ttatctccgg agcccttcca ggacgcgcag
      181 cagcagcccc ttggaaaaac gaatggcgtc ggcggggcag acctcctggc agcagtagca
      241 gcggatgcac ttttcgtagt ctatctcgat gtctcccgcc ttcgtcagct ccatcgcgcc
      301 tgcggggcat atctcaacgc acttgccgca ccggatgcac ttttgcttct cctgaacggg
      361 ccgggacgcc aggaagcgct tgccgaaccg gtcgaggaag ggggggagca ggtggagcgc
      421 gtcgagcttg ggcagctcta tcggtggaat ctcgaactcc tccggaagac cgggggcgtc
      481 ccacctcgca cttttgtcca cgagtccacg ctcccgcgca gccctgtaga gagggaattc
      541 ttccaacgga gcccccagca gcgtacagac gcggaagtca aggtcgagcg ggtccgacga
      601 ggccgcaagg atgccgaaat ctctcggctt cccgttgctt gggccccgtc cctccatgcc
      661 ccggattccg tcgagtatgg tgaggcgtgg agcgaggccg cgcgcgatat ccaggtggag
      721 atcggcgaac gcctcccgcc gaagcccgac gttatagtgc cactgcgcct ttcgctggct
      781 gaccaccgtg ccgaaaagat ttttcacccc cagcgtgagc atcatctgcg cgtgcgtctt
      841 gagcttcggg atgttgatga tcgtatcggc ttcgacggcg cgccgcgata cctcaatgcg
      901 ctggaagacg tttccctccg cgacgggcag cggaacgggg tcgtccagct cgacgcacgg
      961 aatgcccagt tcgcgtgcga cagcctccat cccggtcttc ctcgcgacgt cggagaaacg
     1021 gtctattccg gggctgtctg cgatgaacgg tttccctcct gcctcaagcg tctgcaccgc
     1081 cacggcgcgt acgacctccg ggtgcgtcgt gatgcactcc tcgggcgtcg aggcggaaag
     1141 catattcgcc ttcagcagga cgctgtcccc cggggatata atatttttca tacccccgaa
     1201 ctcttgcaac agcgccgcga gggccttctc gatgtccaat cggctgtatt ttccgcactg
     1261 tgtgaatgtt gttgcgctca ccatcttctc tcctctcgat tcgttttgta taatatacac
     1321 cgaaacagca aacgatgaag tgcatccaca catgggaagg gccggtgaat cgatggctta
     1381 caacgtccag cagttctttc ttcaaaaaag aggcgtgggg tacgtcgccc ccattctgtg
     1441 caccatgcgg ggcttggggg agagcgacat agagcaggcc gtcgtcttcc ataaaaggat
     1501 atccgacgag atcggcgacg cggatttttt cgccgaagaa aacacgatcg cggagagcat
     1561 catgggggaa ggcgtcgtcg tcggggtctt tgcggaggga aaactcatcg cgctccgggc
     1621 cgtctcctat gtcaaagagt ttgtggacga cgcggtcgtc gatctcgacc tggaccccgg
     1681 cgaagcggac catgtcgccg tgatggattt ttgcgttacg gactcatcct tcaggggcaa
     1741 cagtattcag ttctttacgt acctcttcac agagagccag atgtatccga acagatatca
     1801 tctgcacacc acggtgtcg
//
LOCUS       DAOSWW010000597         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_54940_length_1816_cov_3.470187, whole genome shotgun sequence.
ACCESSION   DAOSWW010000597 DAOSWW010000000
VERSION     DAOSWW010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54940_length_1816_cov_3.470187"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PK442_10915"
     CDS             <1..1102
                     /locus_tag="PK442_10915"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO88206.1"
                     /translation="DVRYLAGNDPALFMQRFTPPVLIDEIQYAPELLPYIKMAVDRSG
                     RSGDFWLTGSQSFVMMKNVGETLAGRVGIIRLSGLSTSEIRGIPSKPFTTDPKSLLER
                     AEEVQRLELNALYERIFKGSMPRLYADERVDWETYYRSYIDTYLKRDIKDLTQVADEM
                     AFYNFMTVVAARTSRPVVYEELAGECGISAPTAKKWLSVLVSSDIVAIVQPYFNNILK
                     RVTKMPALHFLDTGLCAYLLKWGNPEALERGAMSGAFFESYVFSEVYKSYLNAGREPP
                     LFYYRDKEKREIDLLLYENGTLSPVEIKKSASPGKDAVKHFRVLEPLSDPEKSGEPAG
                     MKVGIGNGAVVCMANDLLPVDSKNWYVPAWMI"
     gene            complement(1177..>1816)
                     /locus_tag="PK442_10920"
     CDS             complement(1177..>1816)
                     /locus_tag="PK442_10920"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOO88207.1"
                     /translation="RYPDACFGVLIMEGVDNTGKCPALEARKDDLEQRLRSLYSGKSR
                     KEIGAEHPFASYGRYYKAFGKSYHVLFQVESVALKEKPIFSPSPLVAVMFMAELKNGL
                     LTAGHDLDRTEPPFLLDVAKGGETFTSLGGTQRTLPPEDMFFSDRNGILSSIIYGPDD
                     SSAITAATSRALFTVYGTPSISAGEVRAHLEEIEELVHILNPEAARKELLFL"
BASE COUNT          388 a          499 c          539 g          390 t
ORIGIN      
        1 cgacgtcagg tatcttgccg gaaacgaccc tgcgcttttt atgcagcgat tcacacctcc
       61 ggtgctgatc gacgaaattc agtacgcccc ggagctgctg ccctatatca agatggctgt
      121 ggaccgttcc ggacggagcg gcgacttctg gcttaccggt tcccaatcct tcgtgatgat
      181 gaaaaatgtc ggcgaaactt tggccgggcg cgtcgggatc atcaggcttt cggggctttc
      241 gaccagcgag attcggggaa ttccctcgaa gcccttcacg acggatccga aaagcctttt
      301 ggagcgcgcc gaagaagtac aaaggcttga gttgaacgcg ctgtacgagc gcatattcaa
      361 gggctccatg ccgaggttgt atgcggatga gagagtggat tgggagactt attaccgttc
      421 ctacatcgac acgtatctca aacgcgatat aaaggatctg acacaggtgg cggacgagat
      481 ggccttctat aacttcatga ccgttgtcgc cgccagaaca tccaggccgg tcgtctacga
      541 agaactggca ggcgagtgcg gcatttccgc gccgaccgcg aagaaatggc tctccgtgct
      601 cgtctcatcc gatatcgtgg cgattgtgca gccgtacttc aacaacatat taaagcgagt
      661 gaccaaaatg cccgcgcttc actttctgga tacggggctc tgtgcgtatt tgctgaagtg
      721 gggtaatccc gaagcgctgg agagaggggc catgtccggc gcttttttcg agagctacgt
      781 cttctccgaa gtgtacaaga gctatctgaa cgccggaaga gagccccccc tcttttacta
      841 ccgcgacaag gaaaaacggg agatcgatct gttgctgtat gaaaacggaa cgctttctcc
      901 cgtggagatc aagaagtcgg catcccccgg gaaagacgct gtgaagcact tccgggtgct
      961 ggagcccctg agcgaccctg aaaagtccgg cgaaccggcc ggaatgaaag tcgggatagg
     1021 gaacggggct gttgtctgca tggccaacga cctgctgccg gtcgattcga agaactggta
     1081 tgtcccggcc tggatgatat gacgagggaa ctgcggcggt gaaagaagcg ttctctcctc
     1141 cgccggagtt ccctttgctt cgtgcaaagc gatctatcag aggaacagca gctctttgcg
     1201 tgcggcttcc gggttcagga tatgcacaag ttcctcgatc tcctccaggt gcgcgcgcac
     1261 ctctccggcg gaaatagacg gcgttccgta caccgtgaag agcgcgcggg aggtcgccgc
     1321 agtaatggcg ctgctatcgt cggggccgta gatgatgctt gagagaatgc cgttccggtc
     1381 ggagaagaac atgtcctccg gaggaagagt acgttgcgtt ccccccagtg aagtaaaggt
     1441 ttctccccct ttcgcaacgt ccaggagaaa gggcggctct gtccggtcga ggtcgtgccc
     1501 ggcggtgagc aggccgtttt tcagttcggc catgaacatg accgccacaa gcggcgacgg
     1561 cgaaaaaatc ggcttttcct tcaacgccac cgactcgact tggaacagca cgtgatagct
     1621 cttcccgaag gccttgtagt atcgtccgta ggatgcgaag gggtgttcag cccctatttc
     1681 ctttctgctc ttccccgagt agagactgcg gaggcgttgc tcaaggtcgt cctttcgcgc
     1741 ctccagcgcg gggcacttcc cggtattgtc aacgccctcc ataatgagga cgccgaagca
     1801 ggcgtccgga tagcgc
//
LOCUS       DAOSWW010000598         1815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_54995_length_1815_cov_3.371591, whole genome shotgun sequence.
ACCESSION   DAOSWW010000598 DAOSWW010000000
VERSION     DAOSWW010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54995_length_1815_cov_3.371591"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PK442_10925"
     CDS             <1..651
                     /locus_tag="PK442_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOO88208.1"
                     /translation="GAESLFRAKVGALLPLLRRGIGEHVLIVPVRGQNGLMTERAIRI
                     SAEEAVPAPLVPTTWTLAAVAEELRGELYGWGGFLGNRDCSATTRDTLLPFGVWLPRN
                     SRFQAESGRFLTLEGLSNEEKKELLKREGVPFLTLVGLPGHIMLYVGTHRGEPLVFHN
                     MWGIRTERKGVEGRYVLGQTAVTTLDLGEDLPEHAPGKLLIDRINRLGFPAEDGVQ"
     gene            complement(732..1370)
                     /locus_tag="PK442_10930"
     CDS             complement(732..1370)
                     /locus_tag="PK442_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO88209.1"
                     /translation="MKNCPGCGAVIQEDAAFCEYCGVAVKQRAEAGREDSASSWPVEG
                     TWSATPEKTSGASFPQARLKKVSPLIMIFFTLLTFFLYPVVWLFLRLRTFNSMSEKTR
                     IHPVLSGLFACAAVALAVILNGDSLIGPAVPQQAIDTAISYIFLANFILSAVLTFTLR
                     RILREYAARMDKSPLAAKIVARSEVFAFLFQFLYIQHHINLLIDAGLVERTN"
     gene            1548..>1815
                     /locus_tag="PK442_10935"
     CDS             1548..>1815
                     /locus_tag="PK442_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO88210.1"
                     /translation="MRSRSRKLDEEKRRNLMDAAIEEIAANGIEGASYNRIIERSGLS
                     KGVVYYYFENKESLYKTVLEEVERQFIAAVGTLRIPDDKDAFWDV"
BASE COUNT          407 a          467 c          585 g          356 t
ORIGIN      
        1 ggagcggagt cgctcttccg ggcgaaggtc ggcgcgctgc tgccgctgct gcggcggggc
       61 atcggggaac atgtgctcat tgtcccggtg cgaggacaga acggtctgat gaccgagcgc
      121 gcaatccgaa tctccgccga ggaggccgtc cccgcgccgc tcgtccccac gacgtggacg
      181 cttgccgcgg tggcggagga gctgcgcggc gaactttacg gctggggcgg ttttttagga
      241 aaccgcgact gcagcgcaac aacgcgagat acactgcttc ccttcggcgt ctggcttccc
      301 cgcaactcgc gttttcaggc ggagagcggg cgatttctca cgctcgaagg actctcgaac
      361 gaggaaaaga aagaacttct gaagcgggaa ggggttccct ttctgacgct ggtcggtctt
      421 ccggggcata tcatgctcta cgtcggaacg cacagggggg agccgctcgt cttccataac
      481 atgtggggga tacggacgga gcgaaagggt gttgaagggc ggtatgttct cggacagacg
      541 gcggtgacca cgctcgatct cggggaggat cttcccgagc atgctccggg aaagctgctt
      601 atcgacagga tcaaccgtct gggctttccc gcggaggacg gagtgcaata aacacaaggg
      661 agcctcggcg gatgtggtca tacgaggctc ccttgcgtca gaccggccgt tccgccgggg
      721 gcggaagaga atcagttcgt ccgttcaacg agccctgcgt cgatcagcag gttgatgtgg
      781 tgctggatat acaggaattg aaagaggaat gcgaagacct ccgagcgggc gacgattttg
      841 gctgccaggg ggctcttgtc catgcgtgcc gcgtactccc tcagaatccg ccgcagggtg
      901 aaggtgagaa cagccgaaag aatgaagttc gcaaggaaaa tatagctgat ggcagtgtcg
      961 atggcttgct gcggtactgc cggtccgatg agcgagtccc cgttgagaat cactgccagg
     1021 gcgactgcgg cgcatgcgaa aaggccggaa agaacaggat ggatacgcgt cttctccgac
     1081 atggaattaa aggtccgaag ccggagaaag agccagacga cggggtagag aaaaaacgtc
     1141 agcagtgtga agaagatcat aatgagcgga ctgaccttct tgagcctcgc ttgaggaaag
     1201 gaagcgccgg acgttttttc aggcgtcgcg ctccacgttc cttcgacggg ccaggacgaa
     1261 gcggaatcct ctctccccgc ttcggcgcgc tgcttcacag cgactccgca gtactcgcaa
     1321 aacgcggcgt cctcctggat cacggcgccg catcccggac agttcttcat agtgcatcgc
     1381 tccttgaaaa agaatcggac tgtttcatgc cttcgtatca taccacaggc ttcagaggct
     1441 tggatgtatg tgaaattcca gttgatcgat tctcctcttg acaaagtaga ccggatggtc
     1501 taacctgtca gtcgcacttc acggtcgaag tgttgaggag gtgcgatgtg cgttcacgtt
     1561 ccagaaaact ggatgaagaa aaaagaagaa accttatgga cgcggccatc gaggagatag
     1621 ccgcgaacgg tatcgagggg gcgtcgtaca acaggatcat cgagcgttcc ggattaagca
     1681 agggagtggt gtactactac ttcgagaaca aggagagcct gtacaagacg gtgctcgaag
     1741 aggtggagcg tcagtttatc gccgctgtag gaacgttgag aattcccgac gacaaggacg
     1801 ctttttggga tgtgt
//
LOCUS       DAOSWW010000599         1815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_54999_length_1815_cov_3.253977, whole genome shotgun sequence.
ACCESSION   DAOSWW010000599 DAOSWW010000000
VERSION     DAOSWW010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54999_length_1815_cov_3.253977"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK442_10940"
     CDS             <1..482
                     /locus_tag="PK442_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88211.1"
                     /translation="PEIFGSGNVLNIADGGIILTLVFWLLATIFASMLYWSAKYASFS
                     LELLPGAMMLRTLGKEQRIPYIAIESAGIADYRPPKWLRTTLFIASLGSWRMLGHALL
                     LSSRSDWGIELRMKHGDPVKFLCSNLPGAERIVEALRREGVNISPELESVFSERDDR"
     gene            complement(623..1759)
                     /locus_tag="PK442_10945"
     CDS             complement(623..1759)
                     /locus_tag="PK442_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88212.1"
                     /translation="MKNMIMVVSALLAFGVFWVSARPANAGEPSVSVDYEYGDLKEVI
                     VGLPYGMSPSLEAKWFADALKVLPDDEAEYARKTAGMLWTEMINPETGKSETQMLEEE
                     NQAFIAILESLGVKVYRPAEITVGFIKKHYGADVLLNGFSQDFPRDNMAVIGNNVIEF
                     NLRTAIRKVDISGFRDILSEKCSDSTVKWFSMPHTELLEPARADTPFLEGGDVIVLGK
                     TVFVGNTENPSVGSNEAGYKWLKNIVGDRYTVKRIRLVESVLHLDCVLSVPRSGLAIV
                     CEKAFIDGLPEEIKDWDLIRVSLDDVKRLAVNGLPVDSQNYVLSYNLHNDNRFIQTEL
                     ERRGIKVHRVFFGTHNGQGGSLRCATQPLKREVKTAKTHESQGK"
BASE COUNT          358 a          560 c          493 g          404 t
ORIGIN      
        1 cgccggagat cttcggcagc ggcaacgtgc tgaacatcgc agacgggggg atcattctca
       61 cactcgtctt ctggctgctg gcgacgattt tcgccagcat gctctactgg tcggcgaagt
      121 acgcctcgtt ctcgctcgaa ctgcttcccg gcgccatgat gctccggacg ctcggcaagg
      181 aacagcggat tccgtacatc gccatcgaat ccgccggtat cgccgactac cgccctccca
      241 agtggctgcg gacgacgctc ttcatcgcat ccctgggaag ctggcgcatg ctggggcacg
      301 cgctgctcct ctccagccgg agcgactggg ggatcgagct gcgcatgaag cacggcgatc
      361 cggtcaagtt cctctgctcg aacctgccgg gcgcggagcg gatcgtcgag gccctccggc
      421 gcgagggcgt aaacatctcg ccggaactgg agagcgtgtt ctccgaacgt gatgatcgat
      481 aaaatgcaaa aaacgggaga ggcgcaacgc cctcccgttt ttcatgtact cgtcttttaa
      541 ggttctcgat gggggcgcaa tccggcgcag ccttcacctc gaaacggatc tgcccatcgg
      601 caggacgctg tttccgttgc cttcacttcc cttgactttc gtgcgttttc gccgtcttga
      661 cctctcgctt cagaggctgg gtcgcgcagc gcaacgatcc accctggccg ttgtgggttc
      721 cgaaaaatac gcggtggact ttgatgcccc gcctttcgag ctcggtctgg atgaaacggt
      781 tgtcattgtg caggttatac gacagaacgt agttttggga atcgaccgga agcccgttga
      841 cggccagtcg cttgacgtcg tccagactca ctctgatcag gtcccagtcc ttgatctctt
      901 cgggcaagcc gtcgatgaag gccttttcgc agacgatggc aagcccgctg cgcggcacgg
      961 aaagcacgca atccagatgc agcacgcttt cgacgagccg gatgcgtttc accgtatagc
     1021 gatcgcccac aatgtttttg agccacttgt agccggcctc gttggatccc acgctgggat
     1081 tttccgtatt gccgacgaaa acggttttac ccagcacgat gacgtcgccg ccctcaagaa
     1141 acggcgtatc cgcgcgggcg ggttcgagaa gctccgtgtg cggcatggaa aaccacttga
     1201 ccgtcgagtc ggaacacttt tcggacagaa tatcccggaa tccggatata tccaccttgc
     1261 ggattgccgt ccgcaaattg aactcgatga cgttgttgcc gatcacggcc atgttgtcgc
     1321 ggggaaaatc ctggctgaaa ccgttgagca gtacatcggc gccgtaatgc tttttgatga
     1381 agccgacggt aatctccgcc ggacggtaca ctttgacgcc cagagactct aaaattgcga
     1441 tgaacgcctg attttcctct tccagcatct gcgtctcgct tttccccgtt tcgggattga
     1501 tcatctccgt ccagagcatc cccgccgtct tccgggcgta ctccgcctca tcgtccggca
     1561 gcactttcaa cgcgtcggca aaccatttgg cttccagcga cggcgacatg ccgtacggca
     1621 ggccgacaat cacctctttc agatcgccgt attcgtaatc cacggaaacg gaaggttccc
     1681 ctgcgtttgc aggccgggcg gaaacccaaa acacgccaaa cgccaggagc gcagatacaa
     1741 ccatgatcat atttttcata ccatctctcc tcgtgagtgg aaaaaatctt tccttccttc
     1801 atatcggcac gcctc
//
LOCUS       DAOSWW010000600         1804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_55530_length_1804_cov_3.657519, whole genome shotgun sequence.
ACCESSION   DAOSWW010000600 DAOSWW010000000
VERSION     DAOSWW010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55530_length_1804_cov_3.657519"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PK442_10950"
     CDS             complement(<1..891)
                     /locus_tag="PK442_10950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOO88213.1"
                     /translation="MSLPKVYVSRRIQDAGMRLLDGRVEYEVWEEIGPVDRRILLEKG
                     ADADGLLVTLSDRIDDELLSLCPNLKVVSNYAVGYDNIDVDAATRRGVLVTNTPDVLT
                     NATADIAFTLLLASARRMVEANEFLRSGDWVTWHPNLLLGADVSEAVLGIVGMGKIGQ
                     AVARRAKGFDMEILYVNRSPKPEIESMLGARRVSLQELLAKSDFISLHCPLSGETRGL
                     IGEKELRSMKKTAFIINTSRGPVVDQKALFKACSEGWIGGAGLDVFDKEPVPLDEPLL
                     TLKNVTTLPHLGSATIKAREG"
     gene            complement(965..1522)
                     /gene="frr"
                     /locus_tag="PK442_10955"
     CDS             complement(965..1522)
                     /gene="frr"
                     /locus_tag="PK442_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOO88214.1"
                     /translation="MPKTELKELRERMEKVIEHLKNEFLAIRTGRAHPGLVSDIKVDY
                     YGAPTPVKQIATVSIPEGRQIAIAPFDRSALKLIEKAILASSLGVTPQNDGEIIRVNL
                     PELTRDRRIELTKLVGKYAEEGRIALRNLRRDSNDVLKKKEKNSEISEDDLKKYTKEV
                     QDIIDEYIKKVDEVLKAKEKEIMEE"
     gene            complement(1687..>1804)
                     /gene="pyrH"
                     /locus_tag="PK442_10960"
     CDS             complement(1687..>1804)
                     /gene="pyrH"
                     /locus_tag="PK442_10960"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807253.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOO88215.1"
                     /translation="FSLCMENAIPIVVLNILRKGNLKDFLVDGKNTGTMVSA"
BASE COUNT          401 a          517 c          425 g          461 t
ORIGIN      
        1 gccttccctg gctttaatgg ttgcgcttcc taggtgcgga agcgttgtta cgttcttcag
       61 tgtaagaagc ggctcgtcca agggaaccgg ttccttatcg aagacgtcga gtcccgctcc
      121 cccaatccac ccttcggagc acgctttgaa aagcgccttc tggtcgacaa caggaccgcg
      181 cgaggtgttg ataataaatg cggtcttctt catcgagcga agctcttttt caccgatcaa
      241 tcctcgcgtt tcgccgctca atgggcagtg aagcgagata aagtcgcttt tagccagcag
      301 ttcttggaga gagacgcgcc tggcgccgag catgctctcg atttcaggct tcggagaacg
      361 gttgacgtag aggatttcca tatcgaatcc ttttgcccgg cgtgcgaccg cctggccgat
      421 ttttcccatg ccgacgatac ccagcactgc ttctgaaacg tccgcgccca gcagaagatt
      481 gggatgccac gtcacccaat cgcccgaacg cagaaactcg tttgcctcca ccatcctccg
      541 tgcgctcgcc aagagaagcg taaacgcaat gtctgcggtg gcgttcgtca gcacatcggg
      601 agtgttggtc accaagacgc ccctccgtgt agccgcatcg acgtcgatat tgtcataccc
      661 tacggcataa ttgctgacga ctttcaaatt cggacagagt gacagaagtt catcgtcgat
      721 cctatccgaa agagtcacca gcaaaccgtc cgcatctgcg cccttttcga gcagtatgcg
      781 gcggtctacg gggccgattt cttcccagac ttcgtactcg acacgcccat ccaacaggcg
      841 cattccggcg tcctgtatcc tacgtgaaac atatactttc ggaagagaca cgaaaacacc
      901 tcctgcaact ccctgaaaaa agggcggaga aaactccgcc cctgccgatt gtgaatagaa
      961 aaagctattc ctccataatc tctttctctt tcgctttgag aacctcgtct accttcttta
     1021 tgtactcgtc gataatatcc tgaacctctt ttgtatactt tttgaggtcg tcttcactga
     1081 tctccgagtt cttttccttt ttcttcagaa cgtcgttcga atccctccgg aggtttcgaa
     1141 gagcgatacg tccctcttcc gcatatttgc ccaccagttt tgtcagttca atacgacgat
     1201 ctcttgtgag ctccggaaga ttcacgcgaa taatttcgcc gtcgttctgc ggagtgactc
     1261 cgagcgaaga ggcgaggatt gccttttcaa tgagtttcaa agcgcttctg tcgaaggggg
     1321 cgatagcaat ttgacgcccc tccggaatgg agactgtggc aatttgcttg acaggcgtag
     1381 gggctccgta gtaatccacc ttgatgtcgc ttacgagacc gggatgagcg cgtccggtcc
     1441 gaatagccaa gaactcgttc tttaagtgct caatgacctt ctccattctt tctctcagtt
     1501 ctttcagctc agttttcggc attccggaat tcctccttcg atgaagaata gatccccgaa
     1561 aaatgggggc ggaacagtca agcatccagt gcagaactaa aaaacactcc ggcgtctcct
     1621 tcggcatgcg atcagccgac gatacgctgg atttctgcgc gcgcacgtct ctctttcgag
     1681 actctcctac gccgatacca tggttcccgt gttcttcccg tcaaccagaa aatccttcag
     1741 gttgccctta cgcagtatgt tcaatacaac gataggtatg gcattctcca tacagagcga
     1801 gaaa
//
LOCUS       DAOSWW010000601         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_55688_length_1801_cov_3.723368, whole genome shotgun sequence.
ACCESSION   DAOSWW010000601 DAOSWW010000000
VERSION     DAOSWW010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55688_length_1801_cov_3.723368"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PK442_10965"
     CDS             <1..429
                     /locus_tag="PK442_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88216.1"
                     /translation="YELRAVGHNADCSEHTGINVKARMISSMAIAGGLAGAAGALHVL
                     GWTRQAAELAAMEGLGFDGIAVALIGGTSPIGCIPAGLLFGALKYGGSKLQFALRAPI
                     EVVNIVIGSIIFFVAMPKLLRIIRRWLEPKKRQGGDSHAA"
     gene            419..1360
                     /locus_tag="PK442_10970"
     CDS             419..1360
                     /locus_tag="PK442_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO88217.1"
                     /translation="MLLESIAFIIATTLMYSAPLIFTALGGVVSENAGVVNIGLEGMM
                     TLGAFVGAAVGYFANDAWIGFLAAGAAGGALAFFHALACVTFLADHIVSGIAINFIGP
                     GLSLFLSRYFFDGATQTLSLSLDAKIPRPLNGVFEQNGFFDMVFNQYATVYLALFLVA
                     FLWFILYRTRLGLRIRAVGEHPKAADTLGINVLRIKYLAVITSGVLAGFGGAALSVAI
                     VSRFSSTLVSGQGFIALAAMIFGKWKPVGTLAACLLFSSAQGLTVYLGRGDLQRMLPI
                     PSQLLATIPFLLTLAVLIGFVGKSRPPAADGIPYEKE"
     gene            complement(1430..>1801)
                     /locus_tag="PK442_10975"
     CDS             complement(1430..>1801)
                     /locus_tag="PK442_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88218.1"
                     /translation="AQQAGRPYDLGFSMAFFAGVCIGLTTENEVDDDAILAAASQMDK
                     LLAEQSGIAKASDTQRREFAEVLACMAVFALAGHTQAEEAGDDEAVESFKQFARQSMI
                     EILGMDTNRLRMDGRGIRIIQ"
BASE COUNT          328 a          560 c          487 g          426 t
ORIGIN      
        1 tacgagcttc gggccgtcgg tcacaacgcg gactgttcag agcataccgg catcaatgtg
       61 aaggcccgca tgatctcctc gatggccatc gccggaggac ttgcaggagc ggcaggagcg
      121 ctccatgtcc tcggctggac gcgccaggcc gcagaattag cagcgatgga aggtctgggc
      181 ttcgacggta tcgcggtcgc tctgatcggc ggcacctcgc ctattgggtg cattccggcg
      241 ggacttctgt tcggcgccct caaatacgga ggatccaagc ttcagttcgc cctcagagcg
      301 ccgattgaag tggtgaacat cgtcatcggc tctatcatct ttttcgtcgc catgcccaag
      361 ctgctccgga tcattcgacg gtggctggaa ccaaagaaaa ggcagggagg tgattcccat
      421 gctgcttgag agcattgcgt tcattatcgc cacgacgctc atgtactcgg ccccgcttat
      481 cttcaccgcc ctcggcggcg tagtttcgga gaacgcgggc gtggtcaaca tcgggcttga
      541 aggaatgatg accctcggcg ccttcgtcgg agcggccgtc ggctacttcg ccaacgatgc
      601 gtggatcggt tttctcgctg ccggagcggc cggaggagcg ttggcgttct tccacgccct
      661 cgcatgcgtg acgttcctcg ccgaccacat tgtctcgggt atcgcgatca acttcatcgg
      721 ccccggtctc tccctttttc tctctcgcta cttcttcgac ggggcgacgc agacgctctc
      781 actctctctc gacgcgaaaa ttccccgacc gttgaacggg gttttcgagc agaacggctt
      841 ctttgatatg gtcttcaatc agtacgcaac cgtctacctt gctctcttcc tggtcgcctt
      901 tctctggttc attctctata gaacccgtct cggcctgaga attcgcgccg tcggcgagca
      961 ccccaaagcg gcggatacct tgggtataaa tgttctcaga atcaaatacc ttgctgttat
     1021 cacttccgga gttcttgcag gtttcggagg cgccgcgctc agcgtggcta tcgtctctcg
     1081 cttcagctcg acgctcgtga gcggtcaggg gttcatcgcc ctcgcggcga tgatcttcgg
     1141 caagtggaaa cccgtcggaa cgctcgcggc atgccttctc ttcagctcgg cccaggggct
     1201 caccgtttac ctgggacgtg gggacctcca gaggatgctc cctataccct cccagcttct
     1261 ggcgacaatt ccgttcctgc tcacgcttgc cgtgctgata ggattcgtcg gcaagtcccg
     1321 ccctccggcg gcggacggca tcccttacga gaaagaataa cgaaaagagc ccgtcgaaaa
     1381 gtttaatatg ttccgccggg ctcttttttc tagtttcatt gatggtctat tactggataa
     1441 tgcgtatacc ccgaccgtcc atacgaagac ggtttgtatc cattcccaga atctcgatca
     1501 tcgactgacg ggcaaactgc ttgaacgatt ctacagcctc gtcgtcgcct gcctcctcgg
     1561 cctgcgtatg accggcaagc gcgaaaacgg ccatgcacgc cagtacctct gcgaattccc
     1621 ttcgctgcgt atcggaggct ttggctatcc ccgactgttc cgcaagcagc ttgtccatct
     1681 gcgacgcggc tgctagaatg gcgtcatcgt ccacttcatt ttccgttgtc aacccgatgc
     1741 agactccggc aaagaacgcc atcgaaaagc ccagatcata cggacggccc gcctgttgag
     1801 c
//
LOCUS       DAOSWW010000602         1799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_55820_length_1799_cov_2.423739, whole genome shotgun sequence.
ACCESSION   DAOSWW010000602 DAOSWW010000000
VERSION     DAOSWW010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55820_length_1799_cov_2.423739"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PK442_10980"
     CDS             complement(<1..186)
                     /locus_tag="PK442_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOO88219.1"
                     /translation="MMNSAELFERAKRSLVGGVNSPVRSWRGVGGTPLFFQRGEGPFL
                     HSVDGGRYTDYVCSWGPL"
     gene            complement(215..1300)
                     /locus_tag="PK442_10985"
     CDS             complement(215..1300)
                     /locus_tag="PK442_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88220.1"
                     /translation="MVGATDWRGLLYGELLLVVTVTAGLGFGKLLLAASVPERVMRYL
                     LPGAEASRGRGGLHPDLLFALAVSLGSSRAGAALVAEAQREGRLSRREAVFGTLLQSF
                     PGYIKRWLISFPTAFGLAGAAGAIYSLIVLLRSFCRFLFFLALLRKGPSHDLPQGAAS
                     GVPHPPFVPGESATLPGAEPGREDGASAKIRDSILPQQTHEREAVAQGPAGGVHVSRG
                     RALPLFRTLARTLPLACFVYALAYLLSPSIQAFLQEKGASFPLLSSAGWTVAAASFAH
                     VNAALGVAGGAIASGALSTAQGVLALLVGNMLAVLSRVLRQDLAFWLGIFPGRMVRSL
                     FVWNLLTLVVTMAVTVALAAIPVLRGW"
     gene            complement(1452..1769)
                     /locus_tag="PK442_10990"
     CDS             complement(1452..1769)
                     /locus_tag="PK442_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOO88221.1"
                     /translation="MSAHSLASSRAGRFLALGSEPLSVIPGTAPLARIEAMLAAADSA
                     VLYKPSALGEELRSLVERSGPWRTILRIDRAGLSDERIVEGEEALVRSSEYLSVVQLL
                     RWR"
BASE COUNT          352 a          651 c          562 g          234 t
ORIGIN      
        1 cagcggcccc cagctgcaca cgtagtccgt ataccgcccg ccgtcgacgg agtggagaaa
       61 cggcccctcg cccctctgaa aaaagagcgg cgttccgccg actccgcgcc acgagcgcac
      121 cggactgttc acgccgccga caagcgagcg tttcgcccgc tcgaagagtt ccgcgctgtt
      181 catcatttca aaaacctccc ctgaaacacc atatctacca ccctctgagc accgggatgg
      241 cggccaatgc gacagtcacg gccatcgtga cgacgagcgt gagcagattc cacacgaaga
      301 gcgagcggac catgcgcccc ggaaagatcc cgagccagaa ggcgagatcc tgccggagca
      361 ccctggagag caccgcgagc atattcccga cgagcagcgc gagcaccccc tgcgccgtgg
      421 agagcgctcc cgaggcaatc gcgcctcccg caaccccgag ggccgcgttg acgtgcgcga
      481 aggacgcggc ggcgaccgtc catcctgcgc tcgacaacag gggaaaggag gctcctttct
      541 cctgaagaaa agcctggatc gacggcgaaa gcagatacgc cagcgcatag acgaaacagg
      601 cgagcgggag ggtgcgcgcc aacgtccgga agagcggaag agcgcgcccc cggctgacgt
      661 gcacgcctcc ggccggtccc tgagcaaccg cttctctttc gtgcgtctgc tgcgggagaa
      721 tgctgtcccg gatctttgca gaggctccgt cctctctccc cggctccgcc ccggggagcg
      781 tagcagattc acccgggacg aagggggggt gcggaacacc cgacgccgct ccttgcggca
      841 gatcgtgaga cgggcctttc cgcagaagcg cgaggaagaa gagaaaacgg cagaagctgc
      901 gcagcagcac gatgagcgag tagatcgccc cggcggctcc cgcgagaccg aacgcggtgg
      961 ggaaggagat gagccagcgt ttgatgtacc ccggaaacga ttgaagcagc gtcccgaaga
     1021 ctgcttcacg ccgcgaaagc ctgccctccc gctgcgcctc cgccacgagc gccgccccgg
     1081 ccctcgacga cccgaggctc accgccagag cgaagagcag gtccgggtgc aaccctccgc
     1141 gcccgcggct cgcttccgct cccggcagca gatatcgcat cacacgctcc ggcaccgaag
     1201 cggcgaggag cagcttgccg aacccaagtc cggcggtcac ggtgacgacg agcagcagct
     1261 cgccgtagag cagtccgcgc cagtccgtcg cgcctaccat ggcgcacgcc ttctctgctg
     1321 ccgtctccgc gaaaacggcg tacacaggaa gctccgcatg tgcgccgccc ggatgcagct
     1381 atccgcgcgt tcctcgtcgc ggcggccacg tccgttcccc gcggcgaagc gagcgccgtg
     1441 cggacaaggg gtcagcgcca tcgcagaagc tgcaccacgc tcagatactc ggaggagcgg
     1501 acaagcgcct cttccccctc cacgatccgc tcgtcggaaa gccccgccct gtcgatccga
     1561 agtatcgtcc gccacggccc gctgcgctcc accagtgatc gcagctcctc tccgagcgcg
     1621 gacggcttgt agaggaccgc cgagtccgca gcggcgagca tcgcttctat ccgcgcgagc
     1681 ggcgcggttc ccggaatgac cgagagcggc tcgctcccca gcgccaaaaa acgccccgcg
     1741 cgcgacgagg ccagcgaatg tgccgacaaa cgcgagggcg ctcatgttgc ggccctctt
//
LOCUS       DAOSWW010000603         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_55850_length_1798_cov_3.878371, whole genome shotgun sequence.
ACCESSION   DAOSWW010000603 DAOSWW010000000
VERSION     DAOSWW010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55850_length_1798_cov_3.878371"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PK442_10995"
     CDS             complement(<1..640)
                     /locus_tag="PK442_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO88222.1"
                     /translation="MVTLLLETFVLARLKRLQTLSDKIAAEGMIEARVPLEGKDEITA
                     LSRSYNTLLHTLENLLMDIPDAFFLVDPEGVIVMSNEAARAALKQDDGADLRGRDIAA
                     FLGREKLDFYRKFDEPAEVGAGEGSVFEASLLRLDGALVPVEVHRRRIEYGDRTLLLL
                     LARDLTERKQFEERLAQKAYFDAMTGLPNRHSFVRALNRALNAGDALAVAVMN"
     gene            complement(621..1457)
                     /locus_tag="PK442_11000"
     CDS             complement(621..1457)
                     /locus_tag="PK442_11000"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HOO88223.1"
                     /translation="MPLRLKFFLSVVLTLTLVLLMAGFTSNYTTMQAFRNVERRFFQQ
                     DMARVQNRLDAELQMLHHYAADWGAWDSMYFFMDRKDRVRFDEMIDSASLRALDVDLL
                     VLFDTNLLPVVAYTVGPEGEEEALPPALLEDLRRQAPLLSDRASEGAFDDILITGDGV
                     FLAGVSPVLTTYRTGPSRGLLIVGALLEKRLPLMRGSLGTQFDILPLSEDAAPGRSSE
                     DIRIAPNESGGASVWQARGSAVIRIDSPRTIYEKGRDAVMASYLWILLAGMGTHCGDA
                     AA"
BASE COUNT          397 a          548 c          531 g          322 t
ORIGIN      
        1 tgttcatcac cgcgacggca agcgcatccc ccgcgttcaa cgcacggttg agcgccctga
       61 cgaaggagtg ccggttcgga agccccgtca tggcgtcgaa ataggctttc tgggccaggc
      121 gttcctcgaa ctgcttccgc tcggtgaggt cgcgcgcgag gagcagcaaa agcgtccggt
      181 cgccgtactc gatacgccgc cggtgcacct ccacgggaac gagcgcgccg tccaaacgga
      241 gaagcgacgc ctcgaagacg gaaccttctc ccgcgccgac ctccgcgggc tcgtcgaact
      301 tcctgtagaa atcgagcttc tctcttccga ggaatgcggc gatgtcccgg cccctcagat
      361 ccgcgccgtc gtcctgcttc agcgccgcgc gcgccgcttc gttggacatg acgataacac
      421 cctccgggtc gaccaggaag aacgcgtcgg ggatatccat cagcaggttt tccaaggtgt
      481 gcagcagggt attgtacgag cgggaaagag cggtgatctc gtccttcccc tccaagggaa
      541 cgcgggcttc gatcatccct tcggcggcga ttttgtcgga caatgtctga agccgcttca
      601 gccgcgcaag gacaaaggtc tcaagcagca gcgtcaccac aatgagtacc cataccggcg
      661 agcagaatcc acaggtagga agccatcacc gcgtctcttc ccttttcgta aatggtccgc
      721 ggggaatcga tacggattac ggcgctccct ctcgcctgcc agaccgacgc cccgccgctt
      781 tcgttcggcg cgatccggat atcttccgaa gatctcccgg gcgctgcgtc ctctgacagg
      841 ggaaggatgt cgaactgcgt gccaaggctc ccccgcatga gaggaaggcg tttttccaac
      901 agagcgccca cgatcagcag cccgcgggag ggaccggtcc tgtacgtcgt gagcacggga
      961 ctgactccgg caagaaagac gccgtccccc gtaatgagaa tgtcgtcgaa ggcgccctcc
     1021 gaggcccggt cgctcaggag gggggcttgc cggcgcagat cttcgagcag cgccgggggg
     1081 agcgcctcct cctcgccttc cggaccaacc gtataggcga ccaccggaag aaggttcgtg
     1141 tcgaaaagca ccagaagatc gacgtcgagc gcgcgaaggc tcgcgctgtc gatcatctcg
     1201 tcgaaacgaa ctctgtcctt ccggtccatg aaaaagtaca tgctgtccca ggcgccccaa
     1261 tcggcggcgt agtggtgcaa catctgaagc tcggcgtcca gacggttctg aacccgcgcc
     1321 atatcttgct gaaagaagcg gcgctccacg ttgcggaacg cctgcatcgt ggtgtagttc
     1381 gacgtgaaac cggccataag gagcaccagc gtcagcgtca gcaccacgga caggaaaaat
     1441 ttaaggcgta gaggcatcgg cttttctccc cgcgtaaatt tcggagggaa cgcactctcc
     1501 gatcacgcgc ttaccactgc gtttctatta tcggccgaaa gaaggcgttc cgcaacaaaa
     1561 acttccttga cttcaatata acatgaaaac aacggaacga ttgagggaag agagcacttt
     1621 tgtacaattc atccgcgggc gccccgaaga gaatggtgca taccgggacg agaaaacccc
     1681 ggcgactctt caagaaaagg aaaaggggcg gtcgtatgag cggcatgaac gtgttcgaaa
     1741 aatttaatag cggaaagatg cgcatacccg gcggagagag ggagttcgac tctcttga
//
LOCUS       DAOSWW010000604         1793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56146_length_1793_cov_2.319908, whole genome shotgun sequence.
ACCESSION   DAOSWW010000604 DAOSWW010000000
VERSION     DAOSWW010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56146_length_1793_cov_2.319908"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..571
                     /locus_tag="PK442_11005"
     CDS             47..571
                     /locus_tag="PK442_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88224.1"
                     /translation="MDEYELDDDGLGGIGDTFNRFYLLYGTLHGLVALVLHRSIATLL
                     SSNQLSLVQTAQYLQAHWAFFVGCALLFGVAYLFFSANSFYFSSFLGDDRSFRFRAVV
                     AMLGVVTACLFEKFAGRALFGADEHKLLLFLVFFLTDRVLMIPILQGALYWFAEHIFA
                     IAVVIAILFFFFRI"
     gene            657..836
                     /locus_tag="PK442_11010"
     CDS             657..836
                     /locus_tag="PK442_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88225.1"
                     /translation="MAISTAVQKGSTVYVYNEKNGQICAKSGTLMGFTSTTFSVKQGN
                     TVYTYNEKGGMISAH"
     gene            908..>1793
                     /locus_tag="PK442_11015"
     CDS             908..>1793
                     /locus_tag="PK442_11015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOO88226.1"
                     /translation="MVRFVHTADWQIGMKLAKIGDAGKRVREERFSAARRVIDAASAH
                     GAEFILVAGDLFEDNAVDRTQVRMVADILGKAPCPVFIIPGNHDPLVPGAVWEDRSWE
                     AARNVVVFSENTPYELPGATLYPCPLRGKQSDADPTSWIRAEGGDRIHIGLAHGSVEG
                     APIGEWDHPIPRDAASRSGLDYLALGHWHSFGLFGTKDAPRTAYSGTHEPTSFGERDS
                     GNALIVDIEAPGCAPVLTPVRTGGISWISIEEQIDSRDALESVRERIGKLQNPERTLL
                     RIRLSGLMRPDDRDALEEI"
BASE COUNT          378 a          503 c          542 g          370 t
ORIGIN      
        1 cggaatcgcg ccatgcggga atatgaaaga aggaggatga tgctccatgg atgaatacga
       61 actcgacgac gatgggcttg gcggaatcgg agatacgttt aatcgattct atttgcttta
      121 tggaactttg cacggtctcg tggctctggt actgcaccgc agtatcgcca cgctgctgtc
      181 atcgaatcaa ctgagtttgg tgcaaacagc tcaatatcta caagcgcatt gggcattttt
      241 cgtcggctgc gctctcctct tcggagtcgc gtatctgttt tttagcgcga actcgtttta
      301 tttcagctct tttttaggcg acgacaggag ttttcgattc agagcagttg ttgccatgct
      361 gggcgtcgtc acggcatgtc tctttgaaaa gtttgccggc agagcgctct tcggggcgga
      421 tgaacacaag ctgctcttgt ttcttgtgtt ctttctcacg gatcgggtac tgatgatacc
      481 catactccaa ggcgcgctgt actggttcgc ggaacacatc tttgcgatag cggtcgtcat
      541 cgcgattctc tttttcttct tcaggatatg atcgcaaccc ctcgtaagcg gtcgcggtaa
      601 ttgtgtagag tcaatggcgg atggaacatt acggaaaatc gacaggagga gatagcatgg
      661 cgataagcac ggcggtacaa aaagggagca cagtgtacgt gtataacgaa aaaaacggcc
      721 agatctgcgc caaatcgggc acgttgatgg gctttacctc gacgacgttc agcgtcaagc
      781 aggggaatac cgtgtacaca tacaatgaaa aaggaggtat gatttccgcg cattagtacg
      841 acgcgaagag ctacggctca ggtgtattcg atgaagggca tccggtgaat aagaaggagg
      901 gggttgcatg gttcgatttg tgcacacggc cgactggcag atagggatga agctcgcgaa
      961 gatcggcgat gcgggaaagc gcgtccggga ggaacgcttc tccgccgccc ggagggtcat
     1021 cgacgcggcg tccgcacacg gagcggagtt catcctcgtc gcgggagatc ttttcgagga
     1081 caacgcagtc gaccgcacgc aggtgcggat ggtggccgac atcctcggga aggctccatg
     1141 cccggtcttc atcatccccg gcaaccatga ccccctcgtc ccgggagccg tctgggagga
     1201 ccgttcctgg gaggcggccc gcaatgtcgt cgtcttcagt gaaaacactc cgtacgagct
     1261 tccgggcgcg accctctacc cctgcccgct gcggggaaag cagtccgacg cggacccgac
     1321 ctcgtggata cgggcggaag ggggcgaccg gatccatata ggcctggccc acggctccgt
     1381 cgaaggagcg cccatcggcg agtgggacca cccgatcccg agagatgcgg cgtcgcgctc
     1441 cggactcgac taccttgcgc tcggccactg gcactccttc ggactgttcg gaacaaagga
     1501 cgccccgagg acggcctatt ccggaacgca cgaaccgacc tcgttcggcg agcgggacag
     1561 cggcaacgcg ctcatcgtcg acatcgaagc gcccggatgc gcccctgtcc tcacgcccgt
     1621 ccgcacgggg ggcatctcct ggatatccat cgaagagcag atcgactccc gggatgccct
     1681 cgagagcgtc cgcgaacgga tcggcaagct tcaaaacccg gagcgcaccc ttctccgcat
     1741 ccgcctttcc ggactgatgc ggccggacga ccgcgacgcg cttgaggaga ttg
//
LOCUS       DAOSWW010000605         1791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56224_length_1791_cov_3.045507, whole genome shotgun sequence.
ACCESSION   DAOSWW010000605 DAOSWW010000000
VERSION     DAOSWW010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56224_length_1791_cov_3.045507"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK442_11020"
     CDS             complement(<1..195)
                     /locus_tag="PK442_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOO88227.1"
                     /translation="MEVNVVKVGNSKGIRIPAPLLKQLGIGERVILEVEGDALLVRPV
                     RAPRQGWAAAFGRMRRSGDDV"
     regulatory      553..671
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            915..>1791
                     /locus_tag="PK442_11025"
     CDS             915..>1791
                     /locus_tag="PK442_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO88228.1"
                     /translation="MIQKVFFVNGIERNIIVDEETTLAEVLREQLGLTGTKVGCGQGH
                     CGSCNVLMDGKVTRTCVYKMSRLPEGTKIVTIEGIGTPEKLHPLQKAWIKHGAAQCGF
                     CTPGFIVSAKGLLDTNPNPTREEVRDWFQLNRNACRCTGYIPIVDAVMDAAKVLRGEM
                     SEKELEFKMPADGRIWGSKYPRPTAVAKVTGTLDYGADLILKMPKKTLHAALVQATVS
                     HANIKGIDTSEAEKMPGVYKIVTHKDIKGKNRITGLITFPTNKGDGWDRPILCDEKIF
                     QYGDAVAIVCADSYKN"
BASE COUNT          423 a          544 c          492 g          332 t
ORIGIN      
        1 aacgtcgtct ccgctccggc gcatcctccc gaaagcggcc gcccaccctt gcctcggggc
       61 tctgaccgga cgtacgagta acgcatcccc ctccacctcc agaatcaccc gctcccctat
      121 accgagctgc ttcagaagag gggccggaat ccgtatccct tttgaattac cgacttttac
      181 aacattcact tccacagggt gcacctcctc ttcacggaac aaaaagtact tacttcgtat
      241 ccacattcta ccatacagac accacctcga cacgaccacc tccacacaac gcgtcgaatt
      301 tgcgttcatc ggcggaggga actctgaaga actctgtata ccgcgtattt ccggtaccgc
      361 tgcagcgagg ttcttccgcc ccgtctgttc aaaagaacgt gggaaacctt tgaaaagcgc
      421 cgcacaaaat gcaaccatgt tgcaaaatca aaacattttc ccgaaatttc catcctgatg
      481 ttgcccttga atttatgatg ctctataatg agtatcgaag gtacattttt caaaaaaaga
      541 cgactgtttc agtcgagata aaacctccga gtcgcaggcc ctacaggaac aatcccacgg
      601 actgcgaccg ttagggtgca tctggaaacg ggtgcgcctc ccctttggca aggaggattt
      661 ccaaaaacgg catgcgccgt cctctttgca cggaggacgg cgtttttgta ttcttgtttc
      721 acgactcatc gatggaaggg ggagtgcctg ggcaacggta ctgcgttctc ctcttcactg
      781 aggaaaactg gcggaagagt gtggtttgat acggagaagg acggcggctt ccggaccgcg
      841 ttgcttgccg gcgccccggt tcgcggcgcc gccgtcccga cccctgccga gattcggaag
      901 gagctggaaa caggatgatt caaaaggtct tttttgtcaa cggcattgag cggaacatca
      961 tcgtcgacga ggaaacaacg ctcgccgaag tgctccgcga gcagctcggg ctcacgggaa
     1021 ccaaggtagg atgcggccag ggacactgcg ggtcgtgcaa cgtgctgatg gacggcaagg
     1081 tgacccgcac gtgcgtctac aagatgtccc gcctccccga gggcacgaag atcgtcacga
     1141 tcgaggggat cggcacgccg gagaagctcc acccgctcca gaaggcgtgg ataaagcacg
     1201 gcgcggccca gtgcggcttc tgcacgccgg gcttcatcgt ctccgcgaaa gggctgcttg
     1261 acacgaatcc gaacccgacc cgcgaagagg ttcgcgactg gttccagctc aaccggaacg
     1321 catgccgctg caccgggtac attcccatcg tcgatgccgt gatggacgcc gcaaaggtcc
     1381 tccggggcga gatgagcgag aaggagctcg agttcaaaat gcctgcggac ggacggatct
     1441 gggggagcaa gtaccctcgc ccgaccgccg tcgcgaaggt gaccggaacg ctcgactacg
     1501 gcgcggatct catccttaag atgcccaaaa agacgctgca cgcggcgctg gtgcaggcaa
     1561 ccgtctccca cgcgaacatc aagggaatcg acacctcgga agccgagaag atgcccggag
     1621 tctacaagat cgtcacccat aaggacatca agggaaagaa ccgcattacc ggtctgatca
     1681 ccttcccgac aaacaaggga gacgggtggg accgcccgat cctctgcgac gagaagatct
     1741 tccagtacgg cgacgccgtc gccatcgtct gcgcggacag ctacaagaac g
//
LOCUS       DAOSWW010000606         1789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56312_length_1789_cov_3.317186, whole genome shotgun sequence.
ACCESSION   DAOSWW010000606 DAOSWW010000000
VERSION     DAOSWW010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56312_length_1789_cov_3.317186"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1784
                     /locus_tag="PK442_11030"
     CDS             204..1784
                     /locus_tag="PK442_11030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOO88229.1"
                     /translation="MTLEEKVGQIMLVFFEGGAMSENLQKFISEIKVGGVILYSSRNN
                     IESAEQVANLNESIQRRALASSSRPLFIAIDQEGGLIARIREGVTPFPGNMALGAAGD
                     PSLAGRVASVMGEELASLGINMNFAPVVDVNNNPLNPIIGVRSFGSDPQEVARLGAAM
                     ISSFLEKGVFPSAKHFPGHGDTEVDSHSGLPIINVSKDRLNSVEFPPFKAAVNAGVPM
                     VMTAHVLVPALDEKNPATLSPAILGMLRTELNFKGLIITDSMGMAALKKERTMEEAAI
                     EAMNAGADILLFGADKGHEETEHFNMYNALLKACVEGEIPISRLDDAVRKVLEAKEAL
                     GLLQDEWEPHFAPRIAPDGAATAREAALRSITVARNRSETLQTVRDGKKRPLLWPGER
                     IAAGKVFCEFLFDVDLIEVPERPEFHDISDILQRVEGVDRILVAEYDSKRNPDWLNLV
                     RQIGEERTLLISLRTPYALLSLPSVSGAIVSYSDNTPSLQALADVLRGNAPAEGRLPV
                     ELPGFEIRTGGCQVTSGF"
BASE COUNT          415 a          464 c          473 g          437 t
ORIGIN      
        1 gatatagaat acggatttct ttattacagc ggaaactatc ctctttcata tgatcagttt
       61 atccaaaagc tttatgcttc tttaggaggg ggcgccgaat gattacttta aaaaaagctg
      121 tatgcacatt gctcctcggt cttttttttc tcttctccgt gcctttgcga ttctcggaag
      181 caaatacgcg agatctttcc tctatgacgc ttgaggagaa agttggacag attatgctgg
      241 tttttttcga gggaggagca atgtccgaga accttcaaaa gttcatctcg gagatcaagg
      301 tcggaggagt catcctgtat tccagccgaa acaacattga gtccgccgaa caggttgcga
      361 acctgaacga gtcaattcaa cggagagctc tcgcttcaag ttctcgtccg ctcttcatag
      421 ctattgatca ggagggcggt ctgattgccc gtatccgcga gggtgtcacc cctttcccgg
      481 gtaatatggc ccttggagcg gcaggggatc cgtctctggc cggtcgtgtc gcttccgtca
      541 tgggggaaga gcttgcgagc ctcggtatca acatgaattt tgccccggtg gtggatgtga
      601 acaacaatcc gttgaatccg ataatcggcg ttcgctcttt cggctccgac ccgcaggagg
      661 tcgcccgcct cggggcagca atgatttcct cttttttaga aaaaggcgta ttcccctctg
      721 cgaaacactt ccccggacat ggagataccg aggtggattc tcattctggc ctccctatca
      781 tcaatgtctc caaggatcgt ctgaattctg ttgaattccc ccccttcaaa gcggctgtca
      841 atgccggcgt gccgatggtc atgactgcgc acgttcttgt tcctgctctc gacgagaaaa
      901 atccggcgac cctttccccc gccatactcg ggatgctccg caccgaactg aacttcaaag
      961 gcctgataat cacagactcg atgggtatgg cggcgttaaa aaaagaaagg accatggagg
     1021 aagctgctat agaagcgatg aacgccggag cggatatcct gcttttcgga gccgacaaag
     1081 ggcatgaaga aacggagcac ttcaacatgt acaacgcact gttaaaagcc tgcgttgaag
     1141 gggaaattcc aatcagtcgg ctggatgacg ctgtacgaaa ggttctggag gcgaaagaag
     1201 cgcttggtct tcttcaagat gaatgggaac cgcactttgc tccgcgcatc gctcccgatg
     1261 gggctgctac cgcacgcgaa gcggcgttgc gaagtatcac tgttgccagg aacagatctg
     1321 aaacgttgca aaccgtacga gacgggaaaa aacgcccgct gctctggccc ggggaacgga
     1381 ttgctgcggg aaaggttttc tgtgaatttc tcttcgatgt cgacctgatc gaagtcccgg
     1441 aacggccgga atttcatgat atctccgata tactgcagcg cgtagagggc gtcgacagga
     1501 ttctggtcgc ggagtacgat agtaagagaa acccggactg gctcaacctt gtccggcaga
     1561 tcggtgaaga acgaactctc ctcatctcgc ttcggactcc gtacgcactt ttatcgttgc
     1621 cctccgtgtc gggcgctatc gtttcatata gcgacaacac cccttcgctt caggccctgg
     1681 ccgatgtact ccgaggaaat gcccccgctg aaggaagatt gcccgtagag cttcccggtt
     1741 ttgaaatacg aacgggagga tgccaggtaa cctccggttt ttaagctac
//
LOCUS       DAOSWW010000607         1788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56347_length_1788_cov_3.819388, whole genome shotgun sequence.
ACCESSION   DAOSWW010000607 DAOSWW010000000
VERSION     DAOSWW010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56347_length_1788_cov_3.819388"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PK442_11035"
     CDS             <1..747
                     /locus_tag="PK442_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOO88230.1"
                     /translation="RDQLTICRALKLLKDSQVSFNFIFVGARQDHDPSLYDRCVNFCQ
                     KHGLDQLVFFLGQRTDVPSLLKQMDCFVYSSKHDTFGIAVAELASISERRQARLVDTS
                     LSGLPPFLVEDSGLNSGFMIAQYTSAALVSENKVLAHPSSVDSIPTSANQEDHVSMGA
                     FSARKALMILDNARKVLAIELFAASQGLDFSRLLKPGAGTVAAHDCVRSVVPYLKHDE
                     YLHPLIERVEALLSRGAVVRAVEEAIGPLN"
     gene            877..>1788
                     /locus_tag="PK442_11040"
     CDS             877..>1788
                     /locus_tag="PK442_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOO88231.1"
                     /translation="MTDVMRNDDLFPQKNDMPFVNASRGIPRIICLGASAGGLEALER
                     FFRHLPEDVQDIFVIVQHLSPDHKSIMAELLHRYTSLPVVVVDDGMLPQPGRIHLIPP
                     GKEMVVENGAFRLRDRVLREPHLPINVFLQSMAREYGASCVAVILSGTGSDGAQGIKA
                     VHAAGGAVFAQDVESSRFDGMPRNAALTCVVDFVGSPEQIVSALFKTAVDETGEPEIY
                     EERERLCEKDALEPKQLIFRKLSDAFGIDFSQYKDGTILRRLDRRMMMRAVADMSEYA
                     ELLEGDSEEVDHLYRDLLIDVTSFFRNP"
BASE COUNT          352 a          535 c          516 g          385 t
ORIGIN      
        1 agggaccagc tgaccatctg ccgtgcattg aaattgctta aagacagtca ggtgagcttc
       61 aacttcatct ttgtcggtgc caggcaggac cacgacccct cgctctacga ccgctgcgtt
      121 aacttttgtc agaagcatgg actcgaccag ctggttttct tcctggggca aaggaccgat
      181 gtgccttccc tgctaaagca aatggattgc tttgtatatt cctccaagca cgataccttc
      241 ggcatcgccg tggcggagct tgcgagcatc tccgagcgca ggcaggccag acttgtggac
      301 accagcctct ccgggctgcc ccccttcctg gtggaagaca gcggcctgaa cagcggtttc
      361 atgatcgcgc agtacacatc ggcggccctt gtctcggaga acaaagtgct tgcgcacccc
      421 tcctccgtcg actccatccc tacctcggcc aaccaggagg accacgtgtc catgggcgcg
      481 ttctcggcgc gaaaggcgct catgatcctg gacaacgcgc ggaaggtgct cgccatagaa
      541 ctcttcgccg cctcccaggg gctcgacttc agccgtctgc tgaaaccggg cgcgggaacg
      601 gtcgccgcgc acgactgcgt caggagcgtc gttccctacc tgaagcacga cgagtacctc
      661 catccgctca tcgagcgcgt ggaggcgctt ctgagccgcg gcgcggtggt ccgggcggtg
      721 gaagaggcaa tcggaccgct gaactgacaa aagacggaat ctcgaggggc tcgccttcgg
      781 cgggcctctc gccgtatgat cagctctcct ttctcctgcg aagacgggta tgctatactc
      841 tttttcagta cataccgcga aacagggggg atttcaatga ctgatgtcat gcgaaatgat
      901 gatctcttcc cgcagaaaaa cgacatgcct ttcgtgaatg catcccgggg tatcccccgc
      961 atcatctgtc tcggcgcctc ggccggaggc ctggaggctc ttgagcgatt cttccgccat
     1021 ttgccggagg acgttcagga tatcttcgtc atcgtccagc atctttctcc ggaccacaag
     1081 agcatcatgg cggaactgct ccaccggtac acgtctcttc cggtagttgt tgttgacgac
     1141 ggaatgctgc cgcaaccggg acgtatacac ctgattccgc cgggcaaaga gatggtggtc
     1201 gagaacgggg cgtttcgttt gcgggacaga gtccttcgcg aaccgcatct ccccatcaat
     1261 gtttttttgc agagcatggc cagagagtac ggagcttctt gcgtcgccgt catcctttcc
     1321 ggtacgggga gcgacggcgc gcagggaatc aaggcagtcc acgcggcggg tggcgcggtg
     1381 tttgcccagg acgtcgaaag ctcccgtttc gacggaatgc cgcgcaacgc ggccctgacc
     1441 tgcgtcgttg attttgtcgg ttccccggag cagatcgtct ccgctctctt caaaaccgcc
     1501 gtcgacgaaa ccggcgagcc ggagatctac gaagagcgcg agcgtctttg cgaaaaggac
     1561 gctcttgaac cgaagcagtt gatcttccga aagttgagcg acgctttcgg tatcgacttc
     1621 tcccagtaca aggacggcac aattctccgc cgtctggacc ggcggatgat gatgcgcgcc
     1681 gtcgctgata tgtccgagta tgcggagttg ctcgaaggag attctgaaga agtggatcat
     1741 ctctacaggg atctgcttat cgacgtcacg tcgttcttca gaaatccg
//
LOCUS       DAOSWW010000608         1788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56349_length_1788_cov_3.515868, whole genome shotgun sequence.
ACCESSION   DAOSWW010000608 DAOSWW010000000
VERSION     DAOSWW010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56349_length_1788_cov_3.515868"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..777)
                     /locus_tag="PK442_11045"
     CDS             complement(235..777)
                     /locus_tag="PK442_11045"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOO88232.1"
                     /translation="MPRVYYSTILSATVEEAWEVVRDFNGLPKWHPGITASELEDACS
                     PDCEACIGSVRHFQLANGEWMREQLLSLSDLDFSVSYTILETGMKLKNYVATLKLTPV
                     TATDETFAEWEAVFDATDPASEEETAEIVHGVFAEGLENLDDILLEAYEEESGDGCGG
                     CGSGGCGCGSCSCSGDEEEE"
     gene            complement(791..1621)
                     /locus_tag="PK442_11050"
     CDS             complement(791..1621)
                     /locus_tag="PK442_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HOO88233.1"
                     /translation="MKRPTRSELMAKFHDMAKRREPIIGGGAGTGISAKWEEAGGIDL
                     IVIYNSGRYRMAGRGSLAGLLAYGNANDIVKEMAYEVLPVVKKTPVLAGVNGTDPFTI
                     WDKYFDELISLGFAGVQNFPTVGLIDGVFRANLEETGMGYGLEVEAMRAAHERDLLVT
                     PYVFSEEDAVAMAEAGADILVCHMGLTTKGSIGAKTAKTLEECAPLIDSWAEAAKKVR
                     PDILVLCHGGPIAMPEDASYILKHCKHVHGFYGASSMERLPTETAIKAQTQDFKKITF
                     "
     gene            complement(1641..>1788)
                     /locus_tag="PK442_11055"
     CDS             complement(1641..>1788)
                     /locus_tag="PK442_11055"
                     /inference="COORDINATES: protein motif:HMM:NF018498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HOO88234.1"
                     /translation="PEADEALFAALERTVQQSDRRRLVKLPLHVNDPEFAAALVENYR
                     EINP"
BASE COUNT          291 a          557 c          553 g          387 t
ORIGIN      
        1 cccatgcgat ttttacgcgt tcagaaggga gtgcattttt tcagcggtct ccgtttgaaa
       61 cacccgcttt caagtttttc tgcatcaagg atccgaacgg actgaacgtg cagtttttcg
      121 agcggaaaaa gtaaccctgc ggccgagaag gcggttggag gaatcggaac atgaaccggg
      181 atcccctgag gtacaaggga tcccggttat gaacgcattg gcaataggga acaactactc
      241 ctcttcttcg tctccgctgc agctgcaact gccgcagccg catccgccgc ttccgcaccc
      301 cccgcatccg tccccggatt cctcctcgta ggcctccagg agtatgtcgt cgaggttttc
      361 aaggccttcg gcgaagacgc cgtggacgat ctccgccgtc tcttcctcgc tggcggggtc
      421 ggtggcgtcg aagacggcct cccactcggc gaaggtttcg tcggttgcgg tgaccggcgt
      481 gagcttcagg gtggcgacgt agttcttcag cttcattccg gtctcaagga ttgtgtagct
      541 gaccgagaag tccaggtcgg aaagggacaa aagctgttcg cgcatccact cgccgttcgc
      601 gagctggaag tggcgcacgc ttcctatgca cgcctcgcag tcggggctgc atgcgtcctc
      661 aagttcgctc gccgtgattc ccggatgcca tttcggcagg ccgttgaagt cgcggacaac
      721 ctcccacgcc tcctctacgg tggcggagag tatggtgctg tagtacactc gcggcatatt
      781 cgtttcctcc tcagaatgtg atctttttga aatcttgcgt ctgggccttt atggcagtct
      841 ccgtcggcag ccgctccatg gagcttgcgc cgtagaagcc gtggacgtgc ttgcagtgct
      901 tcagaatgta cgaggcgtcc tcgggcatgg cgatcgggcc gccgtggcag aggacgagta
      961 tgtccgggcg gaccttcttc gcggcctcgg cccagctgtc gatgaggggg gcgcactcct
     1021 cgagcgtctt tgcggtcttc gcgccgatgg agcccttcgt ggtcagcccc atgtggcaga
     1081 cgaggatgtc ggctcccgcc tcggccatgg cgacggcgtc ctcctcgctg aagacgtacg
     1141 gggtgacgag gagatctcgc tcgtgcgccg ccctcatggc ctccacttcg aggccgtacc
     1201 ccatgccggt ctcctccagg ttggcccgga agacgccgtc gatgaggccg acggtcggga
     1261 agttctgcac tccggcgaat ccgagggaga tcagctcgtc gaagtacttg tcccagatgg
     1321 tgaaggggtc ggtgccgttg actccggcga ggacgggcgt cttcttcacc acggggagga
     1381 cttcgtacgc catctccttg acgatgtcgt tcgcgttgcc gtaggcgagg agcccggcga
     1441 gcgagccgcg ccccgccatg cggtagcggc ccgagttata gatgacgata aggtcgatgc
     1501 ctcccgcttc ctcccacttc gcggagatgc ccgttcccgc gccgccgccg ataatcggct
     1561 cgcgccgctt cgccatgtcg tggaacttcg ccataagctc cgagcgtgtc ggtcgtttca
     1621 tcagtgaatc cttcctttcg ttacggattg atctcccggt agttctccac gagcgccgcg
     1681 gcgaactccg gatcgttgac gtgcaggggg agcttcacga gacggcgcct atcgctctgc
     1741 tgcacggtac gctcgagcgc cgcgaaaagc gcctcgtccg cctccggc
//
LOCUS       DAOSWW010000609         1785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56512_length_1785_cov_3.172832, whole genome shotgun sequence.
ACCESSION   DAOSWW010000609 DAOSWW010000000
VERSION     DAOSWW010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56512_length_1785_cov_3.172832"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PK442_11060"
     CDS             <1..206
                     /locus_tag="PK442_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88235.1"
                     /translation="DRRDDPERAALERLAARLPLRPFHVPEGTDGERVVAGAMAASSM
                     KGNPVVLPKETLAALWRSVTKTS"
     gene            203..1243
                     /locus_tag="PK442_11065"
     CDS             203..1243
                     /locus_tag="PK442_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOO88236.1"
                     /translation="MTRTNPSAASKHTEKRPRLGLALGAGAARGMAHLGVLRELEEAG
                     LAPDVVAGTSIGALVGGMYAAGKLDRLTEVALGLDWKQIARYFVEISFPRSGLIEGNK
                     LTALLAEIVGEVELTELALPFRAVTTNLMTGEEVVISDGDLVEAIRASIAVPGIFTPA
                     RRGDDLLVDGGLVNPVPVSVCRTMGAERIIAVDLNYGRVKDTRARNSTVKEKSALASQ
                     RPNFDAGQIFEWLERNTKQFDIELPNPLKAWMERHAMPNVFDVLGNTLGIIESQIAAT
                     RLQVDKPDLLIQPNVGDIRFMDFVHAGEMIEEGRRAAKEALGQWKESGSELPDARSPK
                     NASRPKEERRGV"
     gene            1240..1566
                     /locus_tag="PK442_11070"
     CDS             1240..1566
                     /locus_tag="PK442_11070"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HOO88237.1"
                     /translation="MIQESVRTLADFFSFDALVGIHVLRILYFAGAVGIPLVGWIFAR
                     RIRARTPDAAAETFSAFAGTMRRGVFLSFFIFFCFFIEILWRLLCEFLIAFLQMREAL
                     FELTGK"
BASE COUNT          359 a          519 c          582 g          325 t
ORIGIN      
        1 gagaccgccg cgacgatcca gagcgtgctg cgctcgaacg cctcgctgcg cgcctgccgc
       61 tccggccgtt ccacgttccc gaaggcacgg acggtgagcg tgtcgtcgca ggggccatgg
      121 cggcgagcag catgaagggc aatccggtcg tcctgccgaa ggagacgctt gcggctctct
      181 ggaggagtgt gacgaagaca tcatgacgag gacaaaccca tcggcggcct caaagcacac
      241 cgaaaaacgt ccgcgcctcg ggctcgcact gggcgcgggg gcggcgcggg gcatggcgca
      301 cctgggcgtc ctccgcgagc tcgaagaggc cggactcgct cccgacgtcg tcgccgggac
      361 gagcatcggc gcgctggtcg gagggatgta cgcggcgggg aaactcgacc gcctgaccga
      421 ggtcgcgctc gggctggact ggaagcagat cgcccgctac ttcgtcgaaa tatcctttcc
      481 gcgctccggg ctgatcgagg ggaacaagct cacggcgctc ctcgcggaga ttgtcggcga
      541 agtcgaattg acggagcttg cgctcccctt ccgggccgtg accacgaacc tcatgacggg
      601 cgaagaagtc gtcatcagcg acggcgacct tgtggaggcg atacgcgcga gcatcgcggt
      661 tccgggcatc tttactccgg cgcgcagggg cgacgatctg ctggtcgacg gcgggctggt
      721 gaatccggtg ccggtgagcg tctgccgtac aatgggcgcc gagcgcatca tcgcggtgga
      781 tctgaactac ggccgcgtca aggatacccg cgccagaaat tcgacggtca aagaaaagag
      841 cgcgctcgct tcgcaacgtc cgaacttcga cgccggacag atcttcgagt ggctggagcg
      901 caatacgaag caattcgaca tcgagcttcc caacccgctg aaggcatgga tggaacggca
      961 cgctatgccg aatgtcttcg acgtgctggg aaacacgctc ggcatcatcg agtcgcagat
     1021 agcggcgacc agattgcagg tcgacaaacc agacctgctg atccagccca acgtcggcga
     1081 catccgcttc atggacttcg tccacgcggg cgagatgatc gaggagggac gccgtgcggc
     1141 gaaggaagcg ctcggacagt ggaaggaaag tggaagtgaa cttcccgacg cacgttcgcc
     1201 aaagaacgct tccaggccaa aggaggagcg gaggggcgta tgatacagga gagcgtgaga
     1261 accctggccg attttttctc cttcgacgct ctcgtcggca tccatgtttt gaggattctc
     1321 tacttcgccg gggccgtcgg aatccctctc gtcggctgga ttttcgcccg gaggatacgg
     1381 gcgcgcacac ccgacgcagc cgccgagacc ttctccgcgt tcgcaggaac tatgcggcgg
     1441 ggcgtgtttc tttcgttttt catctttttc tgttttttta ttgaaatcct gtggcgcctg
     1501 ctgtgcgaat ttctgatcgc gtttctgcag atgcgcgagg cgcttttcga actcacggga
     1561 aaataacggc tgggcacggc gcggtattcg tggtattgtt cctactgagc tgcaccatac
     1621 cgctacttta ctttaatgga ggaaaacaac atggccttac taccggaaga cgtcggcaga
     1681 gcgtgggaga agagaaaggg agcggtcgtc ctgaccacag tcgacaagga cggcatgccg
     1741 aattcgatct acgctacatg cgtgagcaag tacgacgagg agatg
//
LOCUS       DAOSWW010000610         1785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56516_length_1785_cov_2.832370, whole genome shotgun sequence.
ACCESSION   DAOSWW010000610 DAOSWW010000000
VERSION     DAOSWW010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56516_length_1785_cov_2.832370"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PK442_11075"
     CDS             <1..677
                     /locus_tag="PK442_11075"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOO88238.1"
                     /translation="REFLSCDDWRIELLLSEENEEIQRFRESAVRKVRIREVAEVFRG
                     KSILKKNADPGRIGVLNISDLEGGEIDWGKIATIDEDERKAGRYILREGDVLLSCRGT
                     TVKSAVFRKQEHPVIPSANLLVIRPDASVSGDFLRIFFESPSGDALIRSFQRGTVLMN
                     LNHSDIMEMEMPLPPMERQLEIVGRYFSERKKYEEATRRWEEEKERAYRELLEQVQDA
                     SAESGG"
     gene            734..1699
                     /locus_tag="PK442_11080"
     CDS             734..1699
                     /locus_tag="PK442_11080"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOO88239.1"
                     /translation="MKHYFAYGSNLSFEQMRERCPEHRKIGKGTLEGYKWFLSSRGYA
                     NVINSPEERVHGFVYGISEEDERNLDRFEGVHQGLYRKETLPVRLDEGGCLECLVYID
                     STSPASGEIVLKDWYKQRINRGIEDAELPPEYVRNVIRRFVPAEAKSEGGLPSTTMEG
                     LKALEKVLGCERLPMAREWGGAGLIVTRGEPEKPGNDWRFVRYAATPHAREVSWFIER
                     IRDAFYAEERVDNCSKYEFFGRLGNAAGRCGESVENADARMLCASVLREAYAMYEEME
                     QGRFLCLGIASGGDIADDFAERDFSEKEIRAFFENRGVDASVWKH"
BASE COUNT          429 a          465 c          584 g          307 t
ORIGIN      
        1 cgcgggagtt tctctcgtgc gacgactgga gaatcgagct gcttctctcg gaggagaacg
       61 aggagataca gcgtttcaga gagtccgcgg tgcgaaaggt cagaataagg gaggtcgcgg
      121 aggtctttcg aggaaagtcc atcctgaaga agaacgccga cccgggaagg atcggagtgc
      181 tcaacatctc cgacctggaa ggcggagaga tcgactgggg gaagatcgcc accatcgacg
      241 aggacgaacg aaaggccggg aggtacatac tgcgcgaagg cgacgtcctc ctctcctgca
      301 gagggaccac cgtcaagtcg gcggtcttcc ggaagcagga gcatcctgtc atcccctcgg
      361 cgaacctgct cgtcatcagg cccgatgcat ccgtctccgg cgattttctc aggatattct
      421 tcgaaagccc ttcgggggac gcgttgatac ggagcttcca gagggggacc gtcctgatga
      481 atttgaacca ctccgacatc atggagatgg agatgccgct gccgccgatg gaacgacagc
      541 tcgaaatcgt cgggcggtac ttctccgaaa gaaaaaagta cgaggaggcg acgcggcgct
      601 gggaggaaga aaaggaacgc gcataccggg agcttctgga acaggttcag gacgcatcgg
      661 cggagagcgg cggttgaaga aaaaatgact gccggacgaa ctccgtcaaa aaacacacgg
      721 gaggatgatg cacatgaaac actacttcgc ctacggatcc aacctctcct tcgagcagat
      781 gcgggagaga tgccccgaac acaggaaaat cggaaaggga acgcttgaag ggtacaaatg
      841 gtttctctcc tcccgcgggt acgccaacgt gatcaattcg ccggaggagc gcgtgcacgg
      901 cttcgtctac gggataagcg aagaggacga acggaatctc gaccggttcg agggggtgca
      961 tcaggggctc taccggaagg agacgctgcc ggtgagactc gatgaaggag ggtgcctcga
     1021 atgcctcgtc tatatcgact ccacctcccc ggcgtccgga gagatcgttc taaaggattg
     1081 gtacaaacaa cggataaacc gggggataga agatgcggag ctgccgccgg agtacgtccg
     1141 aaacgtcatc cgaagattcg tccccgccga agccaagtcc gaaggtggac tgccttcgac
     1201 gaccatggaa ggactgaaag ccctcgaaaa agttctcggg tgcgaaaggc ttccgatggc
     1261 tcgcgagtgg ggcggagcgg gtcttatcgt gacgaggggg gagcccgaaa aaccaggaaa
     1321 cgactggcgt ttcgtgcggt acgcggcgac gccgcacgcg agggaggtct cgtggttcat
     1381 cgagaggatc cgggacgcct tctacgccga ggagagggtg gacaactgct cgaagtacga
     1441 gttcttcggc agactcggga acgccgcggg caggtgcggg gagagcgtcg aaaacgcgga
     1501 cgcccgtatg ctctgcgcct cggtccttcg ggaggcgtac gcgatgtacg aggagatgga
     1561 gcagggacgc ttcctctgcc tcggaatcgc ctccggggga gatatagccg acgacttcgc
     1621 cgaaagagac ttctcggaga aggagattcg cgccttcttc gaaaacaggg gagtggacgc
     1681 gtcggtctgg aaacattaga agctcgcctc tgcacatccc caaagtaggt tttctgaaaa
     1741 tacttcggga ggagcgtttg cgtttgatac atctcccact ttcct
//
LOCUS       DAOSWW010000611         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56553_length_1784_cov_3.532678, whole genome shotgun sequence.
ACCESSION   DAOSWW010000611 DAOSWW010000000
VERSION     DAOSWW010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56553_length_1784_cov_3.532678"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..505)
                     /locus_tag="PK442_11085"
     CDS             complement(182..505)
                     /locus_tag="PK442_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO88240.1"
                     /translation="MNALQPVEDKRSIIERLRRERTALASLGVLEIGLFGSFARGEQT
                     PESDVDLLVEFTPQQHTFDSFMDLSFFLEALLGRKVELLTRESLSLYIGERILAEVEK
                     IHGAA"
     gene            complement(728..1006)
                     /locus_tag="PK442_11090"
     CDS             complement(728..1006)
                     /locus_tag="PK442_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO88241.1"
                     /translation="MSVWSVEIDAGTAKKIQKLDRETQRLILSYIDRHLRNGTDPRAS
                     GKALTGPLRGLWRYRIGDYRLLAEIRDDELIIVAVDIGHRSAIYSRRR"
     gene            complement(1003..1242)
                     /locus_tag="PK442_11095"
     CDS             complement(1003..1242)
                     /locus_tag="PK442_11095"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOO88242.1"
                     /translation="MATLEKTLTVRLTPEERMAVEEYAKEKNMTIAQLARESLLEKIE
                     DAYDLEVYTAWLKSKRETVRFEDLVKECGFSEEDL"
     gene            complement(1518..>1784)
                     /locus_tag="PK442_11100"
     CDS             complement(1518..>1784)
                     /locus_tag="PK442_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOO88243.1"
                     /translation="LSAMVLAGVDGILRNADPVANGFSSAEAKQEKVFPLSLNAVLDG
                     LKKDNEWLKPAFPEKLMELWTKRKLEEAEYVYNAPTPQEYELYF"
BASE COUNT          392 a          542 c          444 g          406 t
ORIGIN      
        1 gctccgattc gctggtgttt ccactttcta aagaaagggg ggaagcagtt tgcccctccc
       61 ctttgaagat attcaccgat gattactcct cctgtcgaag tctcacgtgt tcacaaaacg
      121 gcgaagctgt tctctcagtc cggggagctt attgacggcg acatcccgga ctatcggata
      181 ttcacgctgc gccatgtatt ttttcgacct ccgcaagaat gcgttcgccg atgtatagac
      241 tgagcgattc acgggtcagc aactccactt ttcttccgag aagagcctcc agaaagaaag
      301 agagatccat gaacgaatcg aacgtatgct gttggggggt gaattcgaca agaaggtcga
      361 cgtcgctctc cggagtctgt tcgccccgcg cgaaggagcc gaacagcccg atctccagaa
      421 cgcccaagga ggcgagagca gttcgctccc tccgcaggcg ttcgataatg gatcgtttat
      481 cctccacagg ctgcagcgcg ttcattccga tcatctcctt ctccagatct caccggtcaa
      541 tacaaagagt atagcaccgt cgccgaagcg ggaaaaaatg gcaaactcac gtctcttttt
      601 ctgatagacg aaaggcgccg gaacagatcc agctgcacaa atttctcaag agaggtaaga
      661 gagatgctgt tcggactccc tatcacgtca cggagagcgt cctccatcgc ccgcaggagt
      721 acgcagccta tctccttcgg gaatagatcg ccgaacgatg acctatatcc acggcgacga
      781 tgatgagttc gtcatcccgt atctcggcaa gcagacggta gtctccgatc ctgtagcgcc
      841 atagtccgcg cagcggaccc gtgagagctt tcccggaagc tcgcggatcg gtgccgttcc
      901 gaagatgtcg gtcgatgtag ctcagaataa gacgttgcgt ctctcgatcg agtttttgta
      961 tcttcttcgc cgtcccggca tctatttcta ccgaccagac gctcataaat cctcctcgga
     1021 aaacccgcac tccttcacta aatcctcgaa gcgcaccgtc tctcgcttgc tcttcaacca
     1081 tgccgtatat acttcgagat cataggcatc ctcgattttt tcgagaagag actcccgggc
     1141 gagctgggcg atggtcatat ttttctcttt cgcgtactcc tcgaccgcca tacgctcttc
     1201 gggagtgagc cgtaccgtga gtgtcttttc caaagtcgcc atttcagcca cctcctttgt
     1261 attgcatcgt aacacaaagg tatgcgctgt caactccgga cagttgccct cattaaaaac
     1321 ttatatgaaa gaaatcgaag aaattcaaaa accggaagaa atcgttgcat cctgcgacgt
     1381 tgccatcacg ccggacaccc ccgcacgaaa tttccgtacc gccgcggaag cggcgaacac
     1441 aaaacgggag aggctccggc gaagcgtccc cgctccggcc gaagcccctc ccgtccgcgt
     1501 cttcgaattc ccccagccta gaagtacagc tcgtactcct gcggcgtcgg ggcgttgtag
     1561 acgtactcgg cttcctcaag cttccgcttc gtccacagct ccatcagctt ttcggggaag
     1621 gcgggcttca gccattcgtt gtcctttttc aggccgtcga gcaccgcgtt caggctcagc
     1681 gggaagacct tctcctgctt cgcctccgcg ctgctgaagc cgttcgccac cgggtcggcg
     1741 ttccggagaa tgccgtcgac tccggcgagc accatcgccg agag
//
LOCUS       DAOSWW010000612         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56573_length_1784_cov_2.696356, whole genome shotgun sequence.
ACCESSION   DAOSWW010000612 DAOSWW010000000
VERSION     DAOSWW010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56573_length_1784_cov_2.696356"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PK442_11105"
     CDS             complement(<1..554)
                     /locus_tag="PK442_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO88244.1"
                     /translation="MSTSYRNIQSSGSSGGIPAVLYGEPSDRLFLFVHGKLSRKEEAE
                     AFAEDVVPLGYQVLAFDLPEHGERKGENYPCTVQNGVHDLRTVYDSVKDSYSSVSLFA
                     CSLGAYFSLVAYRDVVFDKCLFLSPLLDMERLIRNMMHWANVDETQLQKEREIPTSFG
                     ETLSWDYYEYVRAHPVDRWDSPTS"
     gene            complement(514..951)
                     /locus_tag="PK442_11110"
     CDS             complement(514..951)
                     /locus_tag="PK442_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOO88245.1"
                     /translation="MPGMKEEVNVRLVNEKVQFIGVSPANPEQPLTFDYAPPMGAGAG
                     FNGLELLLMSLSGCSATAVVYLLRKMRKTVSGFEVQAEGIRKETHPIKFEKITLRFIL
                     KSPDAQEEDIQKAVQLTEETYCPVWQMIKNNVDVVPEYTIVRE"
     gene            1372..>1784
                     /locus_tag="PK442_11115"
     CDS             1372..>1784
                     /locus_tag="PK442_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase"
                     /protein_id="HOO88246.1"
                     /translation="MNTKILERIGFDRRFADEASMYPGFYAGRVVSQSKDLYRVTTEE
                     SDLTAEISGKFRFEVRTPSDYPAVGDFVLLDRNEDSSGNAIIHRVLTRKSVFIRRAAG
                     TSNEEQVVAANIDTVFICMSLNNDYNLRRVERYLGV"
BASE COUNT          383 a          471 c          516 g          414 t
ORIGIN      
        1 atcgaggtcg ggctgtccca gcggtcgacc gggtgcgccc gaacatattc atagtagtcc
       61 caggagagcg tctcgccgaa ggatgtcggt atctcacgtt ccttttgcaa ctgcgtttca
      121 tccacattcg cccagtgcat catgttccgg ataaggcgtt ccatgtcgag cagcggcgag
      181 aggaagaggc acttgtcgaa aacgacgtct ctgtaggcga ccaggctgaa gtaggcgccg
      241 aggctgcatg cgaacaaaga gacgctgctg tacgagtctt ttacggaatc gtacacggtt
      301 cgcaggtcat ggacgccgtt ctgaacggtg caggggtagt tttccccctt ccgctcgccg
      361 tgctccggca ggtcgaaggc gaggacctga taccccaggg ggacgacgtc ctccgcgaag
      421 gcttccgcct cttctttccg ggagagcttc ccgtggacga aaaggaacag cctgtcggag
      481 ggctccccgt agaggacggc cggaatgccg ccgctactcc ctgacgattg tatattccgg
      541 tacgacgtcg acattgttct ttatcatctg ccacacgggg cagtacgttt cctccgtaag
      601 ctgcacggcc ttttgaatgt cttcttcttg tgcatccggc gatttcagga tgaagcggag
      661 cgtaatcttc tcgaatttta tcggatgcgt ctccttccgg attccttccg cctgaacttc
      721 gaatccggag acggtcttcc gcatttttcg caacagatag acaacggcgg tggcgctgca
      781 gccgctcaga ctcataagga gcagttcgag cccgttgaaa ccagccccgg ctcccatcgg
      841 aggcgcgtaa tcgaacgtga gcggctgttc gggattggca ggggaaacgc cgataaactg
      901 caccttctca ttgactaacc ggacgttgac ttcttctttc attccaggca tttttcttgc
      961 tccttcgatc ttttagaatg tgattcgttc gagagcacag cctctcgtac agatggcgca
     1021 gagcgcggga aggcgatatc ctcgggcgca gcggcgaaac agtacacgga atagagcact
     1081 atcaaagcca ctcttgaaga aaactttatc cctgcgccct tttaccatat cttatcagtt
     1141 aaatcaagtt ttttgttcaa ttttttgttt attgccgccc ggcgtcttgc aatcacactc
     1201 tttcgtgcta tcatcaggcg gcggaaatta tttggaaaca gaaggagtgg tgacgtatgt
     1261 tgttgaagag ggtcgcgccg gagattgtca aagtccggga ggcgactgca cgctgagttg
     1321 atgtatccgc ctcccgaaag tcattgaaaa atactttttg gagggaaaac agtgaacact
     1381 aaaattttag aacgtatcgg cttcgaccgg cgcttcgccg acgaagcaag catgtacccc
     1441 ggattctacg ccgggcgcgt cgtgtcgcag tcgaaggacc tgtacagggt gacgacggaa
     1501 gagagcgact tgacggcgga gatctcgggg aaatttcgtt tcgaggtgcg cacgccgtcg
     1561 gactatccgg cggtgggcga ttttgtgctg ctcgaccgga acgaggattc gagcggcaac
     1621 gcgatcatcc atcgcgtgct gacgaggaag agcgtcttca tccggagggc ggcgggaacg
     1681 tcgaacgagg aacaggtggt ggcggcgaat atcgacaccg tcttcatctg catgtcgttg
     1741 aacaacgact acaacctgcg ccgggtggag cgttacctgg gagt
//
LOCUS       DAOSWW010000613         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56617_length_1783_cov_2.588542, whole genome shotgun sequence.
ACCESSION   DAOSWW010000613 DAOSWW010000000
VERSION     DAOSWW010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56617_length_1783_cov_2.588542"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1783
                     /locus_tag="PK442_11120"
     CDS             <1..>1783
                     /locus_tag="PK442_11120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO88247.1"
                     /translation="GYATTEGEYYAVRWNERNKLEVARNNAKTGGHSFYYSVTDDLTA
                     GEFVMDAGAFDPRTRNWYIAAKDADGPVFSPIYKHFIMPDLTVSASCPVRAADGVLKG
                     VLGVHTTLSKINGYLEETIRNKGGVAAIVERESGAYVANSMGLPNFSLLPDGRVHRLS
                     AKEGGDAVLKTAYEEYLRTGAHGMKIEDGAEAFFVRMEEYRLDGLDLLIFSAIPERIF
                     TAGVFSSMRLALALVILVLLAAITLYSALASAFMRPMEALIDAAEQFSRGDLEKRATV
                     VRDDEIGRLSRTFNAMADNIYLLVGTLESKVKERTAKLEKANAALNESEDRLRLILDS
                     TAEAIYGIDAEGRCTFANASCLKMLGYGREEELLGRDMHALIHYSQRDGRPIRQDECR
                     IFQVLGMGESAFADDEVFWRADGDSFDVEYSAYPQYKDGKLVGAVVAFMDNTERRKNR
                     AHIDFLTYHDALTGLYNRMYFERALKSLDAEEHLPVTIIFGDVNGLKLTNDVFGHAAG
                     DTLLKRSAEILKKVCRESDVVARVGGDEFAVLLPGSGPEAAKKVIERIRNEFGKEKIL
                     AIKASISMGYAVKTGPDEQLERTVKDAE"
BASE COUNT          393 a          508 c          570 g          312 t
ORIGIN      
        1 ggctacgcca caacggaggg ggaatactac gccgtccggt ggaatgaacg gaacaagctg
       61 gaggtcgcgc ggaacaacgc gaagaccggc ggacactcct tttattattc cgttaccgac
      121 gacctgaccg ccggagagtt cgtgatggac gcgggcgctt tcgacccgcg cacgaggaac
      181 tggtacattg ccgcgaaaga tgcggatgga ccggtctttt cgccgatcta caagcacttc
      241 atcatgcccg acctcacggt gtccgcctcc tgtcctgtcc gcgccgccga cggagtcttg
      301 aagggcgttc tcggcgtgca caccactctc tcgaagatca acggctacct tgaggagacg
      361 atacggaaca agggcggagt cgccgccatc gtcgaaagag agtccggagc gtatgtggcg
      421 aactccatgg gactgccgaa cttttctctc ctcccggatg ggcgcgtgca cagactctct
      481 gcgaaagaag gcggggacgc cgtactgaag accgcctacg aggagtatct tcgcacgggg
      541 gcgcacggca tgaagatcga ggacggcgct gaggcgttct ttgtccgtat ggaggaatac
      601 cgtctggacg ggctggacct tctcattttt tccgccatac ccgaacggat ctttaccgcg
      661 ggcgtcttca gcagcatgcg gctggcgctt gcgctggtca ttctggtact cctggctgcg
      721 atcacgcttt acagcgcgct tgcaagcgcc ttcatgcgcc ccatggaggc gctgatcgac
      781 gccgccgagc aattctcaag aggcgatctc gaaaagaggg cgaccgttgt gcgcgacgac
      841 gagatcggcc gcctttcgcg aacgttcaac gcaatggccg acaacatcta ccttctcgtc
      901 gggacgctgg aatcgaaggt gaaggagcgc accgcgaagc tggagaaagc gaacgcggcg
      961 ctgaacgaaa gcgaagaccg tctccgcctg atcctcgact ccacggcgga ggctatctac
     1021 ggcatcgacg cggagggaag atgcacgttc gccaacgcga gctgtctgaa gatgctgggg
     1081 tacgggcgcg aggaagagct gctcggcagg gatatgcacg ctctcatcca ctactcgcag
     1141 agagacggca ggccgatccg ccaggacgag tgcagaattt ttcaggtgct cgggatgggc
     1201 gagagcgcct tcgccgacga cgaagtcttc tggcgggccg acggagactc attcgacgtg
     1261 gagtactcgg cctatcccca gtacaaagac gggaaacttg tcggcgcggt cgtcgccttc
     1321 atggacaata ccgagcgcag gaaaaacagg gcgcacatcg acttcctcac ctaccacgac
     1381 gcgctcaccg gcctgtacaa caggatgtac ttcgagagag ccctgaaaag ccttgacgcc
     1441 gaggagcatc ttcctgtcac gatcatcttc ggggacgtca acggtctcaa gctgacgaac
     1501 gacgtcttcg ggcacgccgc gggggatacg ctcctgaaac ggtcggcgga gatactgaag
     1561 aaagtgtgca gggagagcga cgtcgtcgcc agggtaggag gcgacgaatt tgccgttctg
     1621 ctgcccggaa gcggtccgga ggcggcgaag aaggtgatcg aaaggatccg gaacgaattc
     1681 ggcaaagaaa agatccttgc gataaaggcg agtatctcca tggggtacgc cgtgaagact
     1741 ggtccggacg agcagctgga acgcactgtg aaggatgccg agg
//
LOCUS       DAOSWW010000614         1782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56669_length_1782_cov_2.763173, whole genome shotgun sequence.
ACCESSION   DAOSWW010000614 DAOSWW010000000
VERSION     DAOSWW010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56669_length_1782_cov_2.763173"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="PK442_11125"
     CDS             <1..1229
                     /locus_tag="PK442_11125"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOO88248.1"
                     /translation="VHGEYVPNEGFKWEIAFGHAKDGRTDLKRFVLNMVADGSGIPLF
                     LELLSGNASDKKSIVVGMEKATNTLAALGESPLFGIADAAFYSAENIARIHGSWISRV
                     PATLNEAAALLETGEALLPCSDPRYSFLEKTSSYGGVEQRWFLFRSDEMAGKQEKTLE
                     KRLERKLADAKKDASALSKTEFFCEADARSAADSFFSEHPLVQGSVLVEEIRRKKGGR
                     GRPASGAEMEIRYRAKIDPALAPDAVEILRSKCGRFILATSAVGETAPGAEEILGLYK
                     EQSAVEKGFRFLKDKSFHAAEIYLKREERIEGLMFLMTIALAVYNLAEKELREVLSAS
                     GESIPAPGGKPMKNPTLDRLFHLFARVTVLVGEECGKRTQRVMNLNDTLKRMLALFGK
                     NFERYYMDGASSNAYG"
     gene            complement(1242..1484)
                     /locus_tag="PK442_11130"
     CDS             complement(1242..1484)
                     /locus_tag="PK442_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88249.1"
                     /translation="MLGNPSAPQESLLQQLENIAALFPDGVDETSNSNKALRAFKSSG
                     ASRDFLEQTYHPKRTLGKIVREGHFRILDPIFRTDI"
     gene            1552..>1782
                     /locus_tag="PK442_11135"
     CDS             1552..>1782
                     /locus_tag="PK442_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88250.1"
                     /translation="MNHTSALHNSIVRLLRQHLESMMDFRNLVTLAWMIFGSIMSKST
                     NRNSWAEYVFGKAERVRSTVRRFERFFDNDRID"
BASE COUNT          405 a          521 c          526 g          330 t
ORIGIN      
        1 gcgtgcacgg cgagtacgtc ccgaacgagg ggttcaaatg ggagatcgcg ttcggccacg
       61 ccaaggacgg cagaaccgac ctgaagcgct tcgtgctgaa catggtcgcc gacggaagcg
      121 ggattccgct ctttctcgaa ctgctctccg gcaacgcctc ggacaaaaag agcatcgtgg
      181 tcggcatgga gaaggcgacg aacacgctcg ccgcactcgg cgaatctccg ctgttcggca
      241 tagccgacgc cgctttctac agcgcggaga acatcgcccg catacacggc tcgtggatca
      301 gccgggtacc cgcgacgctg aacgaggccg cggcgcttct cgaaaccggg gaagcccttc
      361 ttccctgctc cgacccccgc tactccttcc ttgagaagac ctcttcctac gggggcgtcg
      421 agcagcgctg gttcctcttc cgctccgacg agatggccgg gaagcaggag aagacgctcg
      481 aaaaacgcct cgagcggaag ctggccgacg cgaagaagga cgcatccgcg ctgtcgaaaa
      541 cggaattctt ctgcgaggcc gacgcccgct ccgcggccga ttccttcttc tccgagcatc
      601 cgctggttca aggaagcgta ctcgtcgagg agatccgccg caagaaaggc ggaagaggac
      661 gtcccgcgtc gggagcggag atggagatcc ggtaccgagc gaagatcgat cccgcgctcg
      721 ctcccgacgc cgtcgagatc cttcgaagca agtgcggtcg cttcatactc gccaccagcg
      781 cggtaggaga aacagccccc ggcgccgagg agatactcgg gctgtacaag gagcagagcg
      841 ccgtcgagaa gggcttccgc ttcctcaagg acaaatcctt ccacgccgcc gagatttatc
      901 tgaagcggga ggagcgcatc gaaggcctca tgttcctcat gacgatcgct ctggcggtct
      961 acaatcttgc cgagaaagaa ctccgggaag tgctgtccgc gagcggagag agcattcccg
     1021 ctccgggagg gaaaccgatg aagaatccca ccctggaccg tctctttcac cttttcgcgc
     1081 gggtcacggt actggtcggc gaggagtgcg gaaagcggac ccagcgcgtg atgaacctga
     1141 acgacacgct caagcggatg ctcgctttat ttgggaaaaa cttcgaacgg tactacatgg
     1201 acggcgcctc ctcgaacgca tatggataaa tacaaaaatc tctaaatgtc agtgcggaat
     1261 atcggatcga gaatccggaa gtgcccttca cgaacaatct tgccgagcgt gcgcttcgga
     1321 tgatacgtct gctccagaaa atctctggat gcacccgaac tcttgaaggc gcgcagcgct
     1381 ttgttcgaat tcgaagtttc gtcgacaccg tccggaaaca gggcggcgat gttttcaagt
     1441 tgctgaagga ggctctcctg gggtgcgctt ggattcccga gcatgtctaa cagggagcac
     1501 ctggatagtt acgtttgggc aacaaaaaca aatcaagaag gagaatttcg aatgaaccat
     1561 acgtccgcgc ttcataatag catcgtccgt cttctccggc aacacctcga atccatgatg
     1621 gacttcagaa acctggtgac gctcgcatgg atgattttcg gttcgatcat gtcgaaaagc
     1681 acgaatcgga actcatgggc ggagtacgtg ttcggaaagg cggaacgcgt ccggagtacc
     1741 gttcgtcgct tcgagcgctt cttcgacaac gaccggatcg ac
//
LOCUS       DAOSWW010000615         1781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56719_length_1781_cov_2.966976, whole genome shotgun sequence.
ACCESSION   DAOSWW010000615 DAOSWW010000000
VERSION     DAOSWW010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56719_length_1781_cov_2.966976"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PK442_11140"
     CDS             <1..832
                     /locus_tag="PK442_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOO88251.1"
                     /translation="TAGPICRTVEDAVLVLDAIAGYDPADPETAWSAGNVPATYTEYL
                     CKDGLRGKRIGVLRSLFGDKPEHDDVNEAMRRSMEAMKEAGAVLVDVGEPIDTNYLAS
                     KVSVHLHTLKDDLESYLKSLGPKAKYHTLDALIASGEIHPGIVENMKTAAGLSKDSDE
                     YRARTLKRLALRTQVMKLMADLGLDALVYPHQKRLAVPVGESQVERNGVLGSATGFPA
                     ITLPGGFSRPSESAPIGVPIGLEILGRPWSEGTLISIAYAFEQLTHFRKPPKSTPELS
                     "
     gene            975..>1781
                     /locus_tag="PK442_11145"
     CDS             975..>1781
                     /locus_tag="PK442_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOO88252.1"
                     /translation="MNAKRYVGKGIIALLALFMLVPATFAADGKLTLLSLNDIHGRIY
                     SEKDAGGLAKAATVVEKLRATDPGNVFFVQVGDIDEGPLFFYFHGKAEMTGLNAMKAD
                     VGTLGNHEFDLGKEAFFEATGYAEFPIVVSNLRFKDGTPAPFPEYAIKTAANGMKVAF
                     FGLVTPALAYTTKGSDDFRAGQDLPAEAERMVNILSAKEKPDVIVLLSHCGIEADRLI
                     AKNTAGIHAIVGGHSHTLMEEGEFVEGPNGWGTLISQAGSYARHLGVMELA"
BASE COUNT          381 a          540 c          533 g          327 t
ORIGIN      
        1 tacggcgggg cccatctgcc ggacggtcga ggacgccgtt ctggtgctcg acgcgatcgc
       61 agggtacgat ccggccgacc cggagaccgc atggtcggcc ggcaacgtcc ccgccaccta
      121 cacggagtac ctgtgcaaag acggcctgag gggaaagagg atcggggttc tccgaagcct
      181 cttcggcgac aagcccgagc atgacgacgt caacgaggcg atgcgccgga gcatggaggc
      241 gatgaaagaa gcgggcgccg tcctcgtcga cgtcggcgag cctatcgaca cgaactacct
      301 tgcgtcgaag gtgagcgtcc atcttcatac tctcaaggac gacctggaga gctatctgaa
      361 aagcctcggg ccgaaggcga agtaccatac gctggacgct ctcatcgcct cgggcgagat
      421 ccacccgggc atcgtcgaga acatgaagac ggcggcgggg ctgagcaagg acagcgacga
      481 gtaccgggcg cgcaccctca aacgcctcgc gctccggacg caggtcatga agctgatggc
      541 cgatctcgga ctcgacgccc tcgtctaccc gcatcagaag cgccttgcag tccccgtagg
      601 cgagtcgcag gtcgagcgga acggcgttct gggatcggct acgggcttcc cggccatcac
      661 gctccccggc ggcttctccc gtccttcgga gagcgcgccg ataggcgtcc ccatcggcct
      721 tgagattctg gggcgtccct ggagcgaggg cacgctcatt tcgatcgcct acgcatttga
      781 acagctcact cacttccgca agccgccgaa gagcaccccg gaactctcct gaaaccatcg
      841 ttcacgaaaa gacgggccga ggaaactcag cccgtctttt ctcgtcctac caagcgtgtt
      901 ggtgctataa tcaccccgta tccgctcgtc tcgaacacga agcggacaat ttttccacga
      961 ggaggttttt tgctatgaac gcgaaacgat acgtaggaaa aggaattata gccctgctcg
     1021 ctctctttat gctcgttcct gcaactttcg ccgccgacgg aaaactgacg ctgctctccc
     1081 tcaacgacat ccacggacgg atctactccg aaaaggatgc aggcgggctc gcaaaagcgg
     1141 cgacagtcgt tgaaaagctt cgtgcgaccg atccgggcaa cgtctttttt gttcaggtcg
     1201 gggatatcga cgaaggaccg ctctttttct actttcacgg gaaggccgag atgaccggcc
     1261 tgaacgcgat gaaagcggat gtcggtactc tcggaaacca tgagttcgat cttgggaaag
     1321 aagccttttt cgaggccaca gggtacgcgg agttcccgat cgtcgtctcc aaccttcgtt
     1381 tcaaggacgg aaccccggct cccttccccg agtacgcgat caagaccgcc gcaaacggga
     1441 tgaaagtcgc cttcttcggg ctggtcaccc ctgcgctggc gtacaccaca aaggggagcg
     1501 acgacttccg cgccggtcag gaccttcccg cggaggccga acggatggtg aacattcttt
     1561 cggcgaaaga aaaacccgat gttatcgtgc tgttgagtca ctgcggcatc gaagccgacc
     1621 ggttgatcgc gaagaacacc gccggtattc atgcgatcgt cggagggcac agtcataccc
     1681 tcatggaaga gggagaattt gttgaaggac cgaacggatg gggtacgctg atcagccagg
     1741 caggttcgta tgccaggcat ctcggcgtga tggagctcgc g
//
LOCUS       DAOSWW010000616         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56818_length_1779_cov_4.374130, whole genome shotgun sequence.
ACCESSION   DAOSWW010000616 DAOSWW010000000
VERSION     DAOSWW010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56818_length_1779_cov_4.374130"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK442_11150"
     CDS             complement(<1..396)
                     /locus_tag="PK442_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88253.1"
                     /translation="MIFELMSKGNLAQLPDEMCVVGLPETPSPRVLLLLPYNRYLLGT
                     VSLKTYEHWRLGKLGSEESEELILYDDKPKTITLGEVETITIAKEVVDRLKSCLRNQM
                     PPPGEHLPAMLILRGLLREDALFQDEDFLK"
     gene            complement(521..1369)
                     /locus_tag="PK442_11155"
     CDS             complement(521..1369)
                     /locus_tag="PK442_11155"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO88254.1"
                     /translation="MRGKAAVLDAIAAFSRPGWRARTVAAFFSGGLKCIRPRNAVARE
                     NLRMVFPEASEEWIRDIVKKLYEHLAWMVAEYLALLREPQQALEWCEYVEGKAVLDEL
                     KQAGRGAIILTGHVGNWELLAAWLAQSGYPLTAVVRNPDDPNLSEMITAYRTRMGVGS
                     FEKHFVMKEAVRFAKRGGFLGLLPDQAWNATGVMTRFLGHNCYTAGGPAAMAHLARVP
                     VVPVVSYRIAPFRHKVIISSPVPMAEGLDRDDSIQENTERMNKIIEEMILRAPEQWLW
                     LHRRWK"
     gene            complement(1366..>1779)
                     /locus_tag="PK442_11160"
     CDS             complement(1366..>1779)
                     /locus_tag="PK442_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88255.1"
                     /translation="AEELEVFAEGAAKASSTLRNFDESPDEGMKLLSSGNCILLLPRP
                     SSSLALLSAFVALMGIPSVAVRSALMDEMLGKEGYVHLQSENPAEAAHALRSALGEGG
                     RSASAFARRNITDTFSPEKGARKLKTLYLSLGGDQ"
BASE COUNT          441 a          493 c          425 g          420 t
ORIGIN      
        1 ctttaaaaaa tcctcatctt gaaaaagagc gtcctccctg agcagtccgc gaaggataag
       61 catggccggc agatgctccc cgggaggagg catttggtta cggagacagc ttttcaagcg
      121 gtctacaacc tcttttgcaa tcgttatcgt ttcaacttcg ccgagcgtga tagtcttcgg
      181 tttatcatca tagaggatga gctcctccga ctcctccgaa cccagtttcc caagcctcca
      241 atgttcgtag gtttttaaag aaacagtacc gaggagataa cgattatagg gaaggaggag
      301 gaggaccctc ggagaaggag tttcgggcaa gccgaccaca cacatttcat ccggcagttg
      361 tgcaagattt cctttcgaca taagctcgaa tatcatatac atccccccct tttaaaaata
      421 gagtagcatt atttcctaaa agcatcaaga gaagaatacc acatccccct ttttatttcg
      481 caattttctt ttttcagcat tccggaacga ccgttttgtt ctatttccag cggcgatgca
      541 accaaagcca ctgttcagga gcacgtaaaa tcatctcttc tataattttg ttcattctct
      601 ctgtgttttc ctgaatggag tcatctctgt cgagcccctc cgccatgggg acaggagatg
      661 agatgataac cttatggcgg aaaggagcga ttctgtagga aacaacgggt acaacaggta
      721 cccgggcaag atgcgccata gccgccgggc ctcccgctgt gtaacagtta tgtcccagga
      781 aacgcgtcat cactccggtt gcgttccatg cctgatcggg cagcaatcct aaaaatccac
      841 ctctttttgc aaatcggaca gcttccttca taacaaagtg tttttcaaaa gagccaacac
      901 ccattcgagt ccgatacgct gtgatcatct ctgaaagatt cggatcatcc ggattgcgaa
      961 caacggcagt aagcggatat cctgattgtg ccagccaagc ggcgaggagt tcccaattcc
     1021 ctacatgacc ggtcaggata attgctcccc tcccagcctg cttcagctca tcgagaactg
     1081 ctttgccctc gacgtattcg caccattcaa gcgcttgttg aggctcccga agaagagcaa
     1141 ggtactccgc caccatccac gccaaatgct cgtacagttt ttttacgata tcccgaatcc
     1201 actcctccga ggcttccgga aacaccatac gcagattctc cctggcaaca gcgtttcggg
     1261 ggcggataca tttcaatccg cccgaaaaga aggcggcgac ggtacgagcg cgccaccccg
     1321 gacggctaaa agctgcaatg gcgtcgagga cggctgcctt tcctctcact gatctcctcc
     1381 aagagagaga tatagggtct ttagtttcct tgctcctttt tcaggggaga aagtgtccgt
     1441 aatgttgcgc cgcgcaaaag cggaagcgct cctgcctcct tcgcccaatg ccgatcgaag
     1501 agcatgcgcg gcttctgcgg gattctccga ctgcagatga acgtacccct ctttcccaag
     1561 catctcgtcc atcagtgcgg agcgaacggc taccgagggg atccccatca aagcgacgaa
     1621 agccgaaagc agagcaagcg aagatgaggg gcgtggaagg agcagaatgc agttgccgga
     1681 ggagaggagt ttcatccctt catccggcga ttcgtcaaag tttcggagag tgctggacgc
     1741 tttcgccgct ccttctgcaa acacctcaag ctcttcggc
//
LOCUS       DAOSWW010000617         1778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_56875_length_1778_cov_3.138712, whole genome shotgun sequence.
ACCESSION   DAOSWW010000617 DAOSWW010000000
VERSION     DAOSWW010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56875_length_1778_cov_3.138712"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PK442_11165"
     CDS             <1..752
                     /locus_tag="PK442_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO88256.1"
                     /translation="LCLRCLDACPAGGLKLGRLSPREGLVRRGALRFGLGALAALGAA
                     LRARLPDRTVSASASSREERLPISPPGSGSHGNFTKKCVACTACVTACPAGIIRPSLS
                     AWGVQGIFQPELDYSKGYCQFECAACSSACPTGAIAPLTLDEKKRTSIGRARFFRRNC
                     IVRKYGQACGACSEHCPTQAVRMVPFRRNLTIPEVDPTLCLGCGACEYACPAEPKAIT
                     VSGVSVHGKIRDRKNEGAEAPVVAPLEEFPF"
     gene            856..1047
                     /gene="tatA"
                     /locus_tag="PK442_11170"
     CDS             856..1047
                     /gene="tatA"
                     /locus_tag="PK442_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOO88257.1"
                     /translation="MNLGMGEILVLLALALVLFGAKRLPEVGRAAGSAIREFKRGLES
                     GEAPEKAGEDTGEKNEDGK"
     gene            1022..1741
                     /gene="tatC"
                     /locus_tag="PK442_11175"
     CDS             1022..1741
                     /gene="tatC"
                     /locus_tag="PK442_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOO88258.1"
                     /translation="MKRTKTGSDGEFFDHLEVLRRKIIAVLFFFCCATALLFLLSERW
                     VRFLQAPLEGLGVSLYYFKPYEKFLTYMRLSFWGGAALSVPLAVLQAALFVAPALRKN
                     EMKYLILAGGLIPALFLAGAAFAYRFAAPLALRFFLFFGEGDNVLPLWGFGDYASFLF
                     SLLLASGMLFQAPLLLLLFILFGLVSVETLSRLRPWIILGIALIAALLTPPDVVSQIL
                     LGVPLYLLFELVLVLGRFLKR"
BASE COUNT          271 a          531 c          569 g          407 t
ORIGIN      
        1 tactctgtct tcgctgtctt gatgcgtgcc ctgcgggagg gctgaagctg gggcggttat
       61 ccccgcgcga ggggcttgtc cggagagggg cgctccgttt cggtctcggc gcgctcgcag
      121 cgctcggtgc ggccttacgc gccaggctgc ccgacagaac tgtttccgcg tcggcgtcgt
      181 cccgggaaga gcgcctcccg atatcgccgc ccggctcggg gagccacggc aacttcacga
      241 aaaagtgtgt ggcgtgcact gcgtgcgtta cggcctgtcc tgcgggaatc atccgtcctt
      301 cactctccgc atggggggtg caaggcattt ttcagccgga attggactat tcaaaggggt
      361 attgtcagtt tgaatgcgcg gcctgctctt ctgcatgccc aacgggcgcg attgccccgc
      421 tgacgcttga tgaaaagaaa agaacgtcta tcggaagagc gcgttttttc cggcgcaact
      481 gcatcgtcag gaagtacggc caggcgtgcg gcgcgtgctc cgagcactgc ccgacgcagg
      541 ctgtccggat ggttccgttc cgccgcaacc tgaccattcc cgaggtggat ccgacgctgt
      601 gtcttggctg cggcgcgtgc gaatacgcct gcccggccga gccgaaggcg atcaccgttt
      661 ccggcgtctc ggtccacggg aagatccggg accggaagaa cgagggggcg gaagctcctg
      721 tggtggcccc gctggaggag ttcccgttct gaaccttagg gaaagtacga gcgatcacgc
      781 gcgggagcgc tctgccggaa ccgctgcacc gcgctcctgc gccgagaaag gacgcagaga
      841 aggaggggtg ttggcatgaa cctgggaatg ggagaaattc tggtactcct cgcgctcgct
      901 ctggtgctct tcggagcgaa gcgcctgccc gaagtcggaa gggcggcggg cagcgcgatc
      961 agggagttca agagagggct tgagtcggga gaagcgccgg aaaaggcggg agaagatacg
     1021 ggtgaaaaga acgaagacgg gaagtgacgg ggagtttttc gatcaccttg aagtcctccg
     1081 gcgcaagatt atcgccgtcc tctttttctt ctgttgcgcg acggcgctgc tctttcttct
     1141 ctccgaacgg tgggtgcgct tcctccaggc tccgctggag gggctcggag tatctctcta
     1201 ctatttcaag ccctacgaga agtttctgac gtacatgcgt ctttccttct ggggcggcgc
     1261 ggccctgagc gtgcctcttg ccgtccttca ggccgcgctc ttcgtcgcgc ccgcgctccg
     1321 caagaacgag atgaagtacc tcattcttgc aggcggtctt atcccggcgc tctttctcgc
     1381 aggcgccgcg ttcgcgtacc ggtttgcggc gccgctcgcc ctccgtttct tccttttctt
     1441 cggcgaggga gacaatgtgc ttcccctctg gggattcggc gactacgcgt cctttctgtt
     1501 ctcgctgctg ctggcgtccg gcatgctctt tcaggcgccg ctccttctgt tgctctttat
     1561 tctgtttggt cttgtctccg tggagacgct ctccaggcta cggccgtgga tcattttagg
     1621 aattgcgctc atcgcggcgc tgcttacgcc gcccgacgtt gtgtcgcaga tactcctcgg
     1681 agttccgctc taccttctct tcgagctggt ccttgtcctg ggacgttttt tgaagcgttg
     1741 aatttacgct gacgccgcgc tctcatccgg atggtagc
//
LOCUS       DAOSWW010000618         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57140_length_1773_cov_3.941793, whole genome shotgun sequence.
ACCESSION   DAOSWW010000618 DAOSWW010000000
VERSION     DAOSWW010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57140_length_1773_cov_3.941793"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK442_11180"
     CDS             complement(<1..270)
                     /locus_tag="PK442_11180"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HOO88259.1"
                     /translation="MAELVKSEGGSLVGLFHGKGGDLVVGKPFERDIFLFDTYVAGTS
                     YVEGIEELEPHLNVGDALDFFREPDNPYDKRAIVIRNKDGVKIGYV"
     gene            complement(275..1120)
                     /locus_tag="PK442_11185"
     CDS             complement(275..1120)
                     /locus_tag="PK442_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88260.1"
                     /translation="MKLSLEPNGERPDTADAAVIMLSQDFERLKSEVEQLRTELSMLV
                     LERDELLLVECKNIETAYMLSIGALEYKAYEIECAVLRMKRKAELIQAKKNRQEKVVL
                     SKIEELLDIAFAEYQAKLNERIEKMNAAIERSHGRLLSDEETRELKSLYRAVVKALHP
                     DLHPDSSDAKMQLFQNAVAAYGNGDLDGLRIIAAMVAEPAPLYDGPDATTQLTKEKER
                     LSTLLRKVKDRIAEIKTEYPYTMKPVVQSPEKTEARKAELQEYIARLRETLAAYTAKI
                     DEMLR"
     gene            complement(1270..1527)
                     /locus_tag="PK442_11190"
     CDS             complement(1270..1527)
                     /locus_tag="PK442_11190"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO88261.1"
                     /translation="MVNLWKVNLSPEAKKRLQKIPNPDRRRILTALDALYTGPSGDIK
                     PLKGRVEWRLRVGGWRVLLLIDENTATIRVVSIDSRGDVYK"
     gene            complement(1521..1751)
                     /locus_tag="PK442_11195"
     CDS             complement(1521..1751)
                     /locus_tag="PK442_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88262.1"
                     /translation="MTNLIKERNEVRRVLRRLSPRGVERLRTYALHVEMEERQDLEPP
                     LTPEEEECLAISRAEFAEGKEIPLKDVMDELW"
BASE COUNT          317 a          530 c          471 g          455 t
ORIGIN      
        1 gacatagccg attttgacgc cgtctttatt cctgatgacg atcgcccgct tgtcgtacgg
       61 attgtcgggt tcgcggaaga agtcgagcgc gtcgccgacg ttgaggtgcg gctccagttc
      121 ctcgattccc tccacatagc ttgttcctgc aacgtacgtg tcgaagagga atatatccct
      181 ttcaaacggt ttgccgacaa ccaagtcgcc gcctttgccg tgaaacaggc cgacaaggct
      241 gccgccttcg ctctttacca gttccgccat gtgctcacct cagcatttcg tcgatcttcg
      301 ccgtgtacgc ggcaagcgtc tcgcgcaatc gggcgatgta ctcctgaagc tctgccttgc
      361 gggcttccgt cttctccggg ctttgaacga ccggcttcat cgtgtacgga tactccgtct
      421 tgatctcggc tatcctgtct ttcaccttcc gaagcagcgt ggacaggcgt tctttctcct
      481 tggtcaactg tgttgttgca tccggtccat cgtagagcgg cgccggttcg gcgaccatcg
      541 cggcgatgat gcgaagcccg tccaagtcgc cgtttccgta cgcggccacg gcattttgga
      601 aaagctgcat cttcgcgtcg ctcgaatccg ggtgaaggtc cggatgcagc gctttcacga
      661 cagcgcggta caggctcttg agttcgcggg tttcctcgtc ggagagcaat ctgccatggc
      721 ttcgctcgat ggccgcgttc attttttcga tccgctcgtt cagcttcgcc tgatattcgg
      781 cgaacgcgat atcgagaagc tcttcgattt tggagaggac gactttctcc tgccggttct
      841 ttttcgcctg tatcaactcg gcctttctct tcatgcgcag aacggcgcac tcgatctcgt
      901 acgccttgta ttcaagggcg ccgatggaga gcatatacgc cgtttcgatg tttttgcact
      961 cgacaagcag aagctcgtcg cgttcaagga caagcatcga gagttccgta cggagttgct
     1021 ccacctcgga cttcagtctt tcaaagtcct gagagagcat aatgacggcg gcgtccgcgg
     1081 tatcagggcg ttcgccgttc ggctcaagag agagtttcaa ggcatatccc ccatcctccg
     1141 cagcccggaa gagagttgtt gacggcgttg acgatggcga ccacgtgcag cgtggcgatg
     1201 tccgccttga ttaccgtcag catggcgcta ttctccgacc gtgtccgcac ctttttggaa
     1261 gagggggcat catttgtaca cgtctcccct ggaatctata gatactactc gaatcgtcgc
     1321 cgtattttca tcgatcagca acaaaaccct ccagcctccg actctgagtc tccattcaac
     1381 acggcccttc agcggcttga tgtcgccgga agggccggtg tacagcgcat cgagcgcagt
     1441 caatattcgt cgtcggtcgg ggttcgggat tttttgcaag cgtttcttcg cctccgggga
     1501 gaggttgact ttccagagat tcaccatagt tcatccatga cgtccttcag cgggatctcc
     1561 tttccttcgg cgaactcggc tctcgaaatc gccagacact cctcttcctc cggagtcagg
     1621 ggaggctcca aatcctgccg ctcctccatt tcgacgtgga gcgcatacgt ccgcagacgt
     1681 tccacccctc gcggggaaag tcgccgaaga acgcggcgta cttcgttgcg ctctttgata
     1741 agattggtca ttggcaatcc cccttctttt atg
//
LOCUS       DAOSWW010000619         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57468_length_1767_cov_3.507593, whole genome shotgun sequence.
ACCESSION   DAOSWW010000619 DAOSWW010000000
VERSION     DAOSWW010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57468_length_1767_cov_3.507593"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..1238)
                     /locus_tag="PK442_11200"
     CDS             complement(354..1238)
                     /locus_tag="PK442_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOO88263.1"
                     /translation="MTEDTRPATSRETRLPEAKGFSKRRTRSIDGVLGAVAGDVIGSV
                     YEWHAIKTETFPLFSPSSRFTDDSIMTVATADALLTDGDYARAYRSWGRAWPDAGYGG
                     NFSRWLRKAHASPYGSFGNGSAMRASPAAYAADSLEEVLRIAEATSVVTHDHPQGILG
                     AQALAGSVFLARRAASNGTGASCKEEIRVFLEHRCGYSLRKTVSEIRPDYTFDVTCQG
                     SVPESLICFLEASDFESAVRKAVSLGGDADTQACIAGAVAGALWGVPEHIWKRVRSTL
                     DASMLRILDAFEERFAKE"
BASE COUNT          353 a          535 c          508 g          371 t
ORIGIN      
        1 cgatcggcgc ccaaccgtcc ccctcctgaa caagcgcggc cgatgcggcg ggaaggaaca
       61 gaagcggcag gagcagcgcg aaaagaacaa agcgcggaac tcgagcgcgg cgcggcttcg
      121 tcaaatacat gtgtatccac ctccctcggg acagtatggt gtacagtcag cagcacaaga
      181 atattcgccc tgtggtgaaa tgtcaaactt cacattgtgc cttcgtacgt gtcttctccg
      241 gcgaatccgg cggcggtcgt ttccaaaacg ctccacagga gcacaggatt ttctccacaa
      301 aaaaagagac cttttcaggc ctcttcaggg gtgttcaact gtacggtcgc atgctattcc
      361 ttcgcgaagc gctcctcgaa agcgtccaga attcggagca tggatgcgtc gagcgtgctc
      421 cgcacccgct tccatatatg ttccggcacg ccccaaaggg ctcccgctac agcgccggcg
      481 atgcatgcct gcgtatccgc atccccgccc agcgagacgg ctttgcgaac ggcgctctcg
      541 aagtccgacg cctcgagaaa acagatgaga gattccggca cgctcccctg gcaggtgacg
      601 tcgaacgtgt agtcgggacg gatctcggat acagtcttcc gcagactgta tccgcagcgg
      661 tgctcgagga aaacgcggat ttcctccttg caggacgccc cggtcccgtt cgatgcggcc
      721 ctgcgggcga gaaagacgct gccggcaagc gcctgcgccc cgaggattcc ctgaggatgg
      781 tcgtgcgtca cgacggaagt cgcttccgcg atgcgcagca cctcttcaag agaatcggcg
      841 gcgtacgcgg cggggcttgc gcgcatcgcg gagccgtttc cgaagcttcc gtagggcgac
      901 gcgtgcgctt ttctcagcca ccgcgagaag ttgccgccgt atcctgcgtc gggccacgcg
      961 cggccccagg aacgatacgc gcgggcgtag tctccgtcgg taagcagcgc atcggccgtc
     1021 gcaacggtca tgatgctgtc gtcggtgaaa cgggaagagg gactgaagag gggaaacgtt
     1081 tccgtcttta tggcgtgcca ctcgtacacc gagccgataa catctcccgc aacggctccc
     1141 aatactccgt cgatcgaacg ggtccggcgc tttgaaaacc cctttgcttc cggaagccgt
     1201 gtttcccgag aagttgccgg gcgcgtgtct tctgtcatgc aatcaccttc ctcgcataaa
     1261 aggtagaggg aaaaaagaga agcggcgaca gccgggggtg agaatgcccg ggaaagaacg
     1321 ggcgcacctg ttccggattt ttccgtcaaa cgtccggccc gcgaaggccg tgacatcggt
     1381 ttcttgagac ttcactcttg agtcgtgatc ccctggttac cgacgcgggg ccgtcttaat
     1441 cttgagtcgt cagtcgtgag ccttgaggcg catacgctag gagcgtatca tgcgtcgcct
     1501 gccgcttcgc gaatctgaag gttcgtcttg accccgacaa gggtctttcc caattttctt
     1561 tttacatcgc cgcttcgtcc ttattcgcat gtcaaggtgc cgggtgtttc aggctgcgcg
     1621 gagggaatcg cctccgcgcc tttttactct tccatttcga tcagatcggt cgtggcattg
     1681 atgacttcaa gcctcgaatc aatcatatcg gccaaatcct gccactttct gatactatcc
     1741 agtttttccg ttcgtcgtaa agaacct
//
LOCUS       DAOSWW010000620         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57472_length_1767_cov_3.400117, whole genome shotgun sequence.
ACCESSION   DAOSWW010000620 DAOSWW010000000
VERSION     DAOSWW010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57472_length_1767_cov_3.400117"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PK442_11205"
     CDS             <1..911
                     /locus_tag="PK442_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOO88264.1"
                     /translation="EFSAACMSAGFPDEEQFLAAELPSERKAELAAAAKDLDERRTEL
                     RARQKDRKTKLAAEIDRKVTETSLEELEPRYAQFEASLKALRDVIAGLKHTLNENTAA
                     KARVQEKQAAIEARKKECRRWETLHELIGSSDGKKYRNFAQGLTFDLMVGHANRQLQK
                     LTDRYLLIRDEAQPLELNVVDNYQAGEIRSTKNLSGGESFLVSLALALGLSQMAGRNV
                     RVDSLFLDEGFGTLDEEALDSALETLASLQQDGKLIGVISHVPALKERIGTQIQVTPQ
                     TGGRSRIFGPGCSGAGAAERAGEGGS"
     gene            complement(1205..1417)
                     /locus_tag="PK442_11210"
     CDS             complement(1205..1417)
                     /locus_tag="PK442_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88265.1"
                     /translation="MNTEYTAVMKQDGDWWIGWIEEIPGINCQERTRDELTETLRITL
                     REAIELNRSDAVDAAGEGYTKVKIAV"
     gene            complement(1555..>1767)
                     /locus_tag="PK442_11215"
     CDS             complement(1555..>1767)
                     /locus_tag="PK442_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-lysine deacetylase"
                     /protein_id="HOO88266.1"
                     /translation="VRKNGLSPRLLAKGGTADFNLAAAWNCPLAAYGPGDSKLDHTAE
                     EHISLSEYLTSLDILTDAIPMIMRSA"
BASE COUNT          371 a          488 c          552 g          356 t
ORIGIN      
        1 gtgagttctc ggcagcgtgc atgtccgctg gttttccgga tgaagaacag tttctggcgg
       61 ccgaattgcc ctccgaaagg aaggccgagc tggcggccgc ggccaaggat cttgatgagc
      121 gccgaacgga gctgagagcc aggcagaaag accggaaaac gaaattggct gcggaaatag
      181 accggaaggt taccgaaacg tcgctggaag agcttgaacc gcggtatgcg cagttcgaag
      241 catcgctgaa agcgctccgc gacgtcattg ccggtctcaa acacacgctg aatgagaata
      301 cggccgccaa agcgcgggta caggagaaac aagcggcgat cgaagcccgg aaaaaagagt
      361 gtcgcagatg ggaaaccctg cacgaattga tcggctcctc ggacggcaaa aagtaccgca
      421 acttcgccca ggggctgacc ttcgatctga tggtcgggca cgccaaccgg caactgcaga
      481 agctgaccga ccgctacttg ctgattcgcg acgaagccca acctctggaa ttgaacgtag
      541 tcgacaacta ccaggcgggg gagatccggt ccacgaagaa cctttccggc ggcgagagtt
      601 ttctcgtcag tctggccctg gctcttggtt tgtcgcagat ggcgggcagg aatgtccgcg
      661 tggattcgct ctttcttgac gagggcttcg gtacgttgga cgaggaagcc ctcgacagcg
      721 ccctggaaac cctcgcaagt ctccagcagg acggcaagct gatcggcgtg atttcacacg
      781 tacccgcctt gaaggagcgc atcggtacgc agatccaggt aacgcctcaa accggaggca
      841 gaagccggat tttcggtccc ggatgcagcg gggcgggtgc tgcagagcgg gctggagaag
      901 ggggaagtta aaaacggcaa gaagggcgga aagagactgt acagtcatgc gcaacaaaat
      961 tttcgtcgga gcggactttc gccggacggc gttcgggccg tcgagtatct tggcctgtcc
     1021 tattgaacgg gcggcactcc gagatctttg cagatcttct tcgccaggag attcgatatc
     1081 tccgagtgtc gcggaacagc tgagcgcgta ttcaaggagg cgttgtgcca ccatgagtgg
     1141 ttggcgcctt cccgtaacag gacgcacccc tgtccgcgca ggtagcgaag caacacttcc
     1201 cgcttcatac cgcgattttt accttcgtgt agccttcgcc cgccgcatcg accgcatcgc
     1261 tccggttgag ttcaatcgct tcgcgcaggg tgatgcgcag cgtttccgtc aactcgtcgc
     1321 gagttctctc ctggcagttg attcccggga tttcctctat ccagcctatc caccagtcgc
     1381 cgtcctgctt cattacggcg gtgtattccg tattcatgta catcgctcct ttactcttcg
     1441 cgatggttgc cgggtgctac tccctgcatc gagtcggcca cgcgctcttg aatagagaaa
     1501 gcatagtatt gatgcggaat aattgcaagc gtatgcgcat catggggagc gcgttcaggc
     1561 gctcctcatg atcatcggaa tggcgtccgt cagaatgtcg agggaggtca ggtactcgga
     1621 gagcgaaata tgctcttcgg cggtgtggtc gagcttgctg tcgccggggc cgtaggcggc
     1681 cagggggcag ttccacgcgg cggcgaggtt gaagtcggcg gtgccgccct ttgcaaggag
     1741 cctgggactc aggccgttct tccggac
//
LOCUS       DAOSWW010000621         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57482_length_1767_cov_2.927570, whole genome shotgun sequence.
ACCESSION   DAOSWW010000621 DAOSWW010000000
VERSION     DAOSWW010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57482_length_1767_cov_2.927570"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..>1767
                     /locus_tag="PK442_11220"
     CDS             364..>1767
                     /locus_tag="PK442_11220"
                     /inference="COORDINATES: protein motif:HMM:NF019520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOO88267.1"
                     /translation="MIVADLLKKNLFPDFQILAGSGGVGREISAVSVLEAPDADRWMR
                     GGEFLVGSGFVFKDDPEQLTPLLRRMNEKGVAAIGFKLDRFHHKLPASMTAEADALQI
                     PILEIPMHYRWTDIIELIHTILVQERLKERPDDLTSFWEESLDYKKLMSGFAQNLGRD
                     LLVQAPQLELNNLFLADGSILGGEAVQKALDTPLVQEQPLPKKGQIHSCVEVRNSGTI
                     RRFAAYRLRLDTPISIFMILSPGELAPSARQERMLLRALTMLRASALEIAILSSKKVL
                     KKERFLEGLCFGMYCDPNMIRINLQELGIPLSLPVTVLIVSTADGLSTPRWSSPSQLS
                     YRLGNTWVTLIDESETAAWKKSLAPVAEQAGFHFSFGGKAADLMEIPRSYQEARRTFS
                     LLRDFALPPGVYLYEELSLYGLLQSMTKLPEAKDVWTRYWSPLEEDTSQARRSLPLKD
                     LAAMLVARDFNARSCADG"
BASE COUNT          383 a          551 c          485 g          348 t
ORIGIN      
        1 tacaagtcct tccaacggtt tcataccagt tccacctccg aaatgatgac cggaacaaaa
       61 agcgcaggct tttcatccgg ttcgaatctc tcgtctctct ccgaaaagtc gttccatgac
      121 aagaagcgat ttccggaaaa aagatcttcc aatgtgatac aacgccagcg agacttctca
      181 gtcttctttt actctcaaac tgtggaaacg ctcaaaagcg ctgaatcaga ttttacaatg
      241 gacttatttt atcataaaat atgtttggag cagaggaaat gcaatgtcat cacatgacaa
      301 aaattcagcc aacaaccccc aatggatgac atccttcaaa cggggcaaaa agggagagtt
      361 tacatgatcg tcgccgacct gttgaaaaaa aacctgttcc ctgactttca gatactcgcc
      421 ggtagcggcg gcgtcgggcg cgaaatcagc gccgtctcgg tcctcgaagc tccggacgcc
      481 gaccgctgga tgcgcggcgg ggagtttctg gtgggaagcg gcttcgtctt caaggacgac
      541 ccggaacagc tcacccccct cctccgccgg atgaacgaga agggagtcgc cgccatcgga
      601 ttcaaactcg accgctttca tcacaaactc ccggcgagca tgaccgcgga ggcggacgcg
      661 cttcagatac caattcttga aattccgatg cactatcgct ggaccgacat catcgaactc
      721 attcacacca tcctcgtcca ggagcggctg aaagagcgcc cggacgatct gacctctttc
      781 tgggaggaga gcctcgacta caagaagctg atgtccggat tcgcgcagaa tctcggcaga
      841 gatcttctgg tgcaggcgcc ccagctcgaa ttgaacaatc ttttcctcgc cgacggaagc
      901 atcctcgggg gcgaagccgt gcagaaagcc ctggacacgc cgctcgtcca ggagcagccc
      961 ctccccaaga aggggcagat ccactcgtgc gtggaggtgc gcaacagcgg gaccatccgg
     1021 cgcttcgcgg cctaccgcct gcgcctggac actcccatct ccatcttcat gatcctctcg
     1081 cccggagagc tcgccccgtc ggccaggcag gagcggatgc tgctccgcgc cctgacgatg
     1141 ctgcgggctt ccgcgctgga gatcgccatt ctctcaagca agaaggtatt gaaaaaggaa
     1201 cgctttctgg aggggttgtg cttcggaatg tactgcgatc cgaacatgat ccggatcaac
     1261 ctgcaagagc tcgggattcc gctctcgctt cccgtgacgg tgctcatcgt ctccacggcc
     1321 gacgggctct ccactccgcg gtggtcgtcc ccctcccagc tgagctaccg gttggggaat
     1381 acgtgggtca cgctcatcga cgaatcggag acggccgcat ggaaaaagag cctcgcgccc
     1441 gtggcggagc aggccgggtt ccacttctcc ttcggcggca aggccgcgga tttgatggag
     1501 atcccccgct cataccagga ggcgaggcgg accttttccc tgctgcgcga cttcgcgctt
     1561 ccgcccggcg tctatctgta cgaggagctt tcgctctacg ggctgctcca gagcatgacg
     1621 aagcttccgg aggcgaagga cgtctggacg cgctactggt cccccctcga agaggacacg
     1681 tcgcaagccc ggcgaagcct gccgctgaag gatctggcgg cgatgctcgt cgctcgcgac
     1741 ttcaacgccc gttcctgcgc cgacggc
//
LOCUS       DAOSWW010000622         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57711_length_1762_cov_3.564148, whole genome shotgun sequence.
ACCESSION   DAOSWW010000622 DAOSWW010000000
VERSION     DAOSWW010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57711_length_1762_cov_3.564148"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1762)
                     /locus_tag="PK442_11225"
     CDS             complement(<1..>1762)
                     /locus_tag="PK442_11225"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO88268.1"
                     /translation="TEEPLAPSIEPDETGVSVSVQEESGESRSAVSTFTIPAHPLYPL
                     IEEDSAPEKTFFVETNRTHAQPQREKNTKNRVLPICLLAAALVMGGLFFASGESFEKI
                     LQQGDQAYAQKEYDRALLLYEKAAEKNPLRAEPLFGKARTLEELDRKGEAVHAWNSCL
                     KIAPEDAEVYRRLGDLLFELGSLDNAIRNYQESVRFAPEDSETHFRLGSLLESREDFS
                     QALESYRKALEVDATRDDFAEAVERTEKKVLIADEERRQRTALSEEQIRLGAAFLVTG
                     EFLEAEAHFLRALDLIPEGEDAFLGLAEARIGKGDIEGARETYLRLLEVTPQSAKARA
                     ALLALDPSANDSKEPVLSDDRSMPIAEQQGKTDAGQLSPDFVSSSITSSSDVSNKTEQ
                     SADVAVLRIPGEKNTAEALGEEGSTQAVIPPGSGVTPPPVSADIEIASLPSSGDVLPA
                     KPSPVVEITEKNDFPQALVLTPETTPLKETPLSSDVSSVIGEVVSKEPKERPSKRTET
                     PRAPFNGRVVKSTVSPPLSVPRRSRKSSAPIETGQNTRSIAAFKKNEERILEASRSYN
                     LSGQYLKAAEKASEALDFQQS"
BASE COUNT          411 a          476 c          433 g          442 t
ORIGIN      
        1 agattgctgg aagtcaagag cttcggacgc cttttccgcc gctttcaaat actgtccgga
       61 gagattgtac gaacggcttg cctcaagaat tctctcttca ttcttcttaa acgccgctat
      121 agaacgagta ttttgcccgg tttcaattgg cgcagaactc ttccgggaac gtcgcggaac
      181 agataatggc ggcgataccg tcgatttgac cactcgtccg ttgaacggcg ccctcggcgt
      241 ttcagttctc tttgaagggc gctccttcgg ctctttggaa acgacttccc ctatcaccga
      301 ggagacgtcg cttgaaagcg gcgtctcctt cagaggcgta gtctcaggag taagaacaag
      361 cgcctgagga aaatcgttct tttccgttat ctcgacgaca ggagacggct tcgccggaag
      421 aacgtcgccg gaggaaggga gagaagcgat ctcaatatct gctgaaacgg ggggcggcgt
      481 cacaccgctt cccggcggaa tgacggcttg ggttgatccc tcttctccca gggcttctgc
      541 tgtgtttttt tcacccggta tccgcagaac agcaacatcg gcagattgct cggttttatt
      601 cgatacgtct gaggaactgg taatacttga tgaaacaaaa tcgggagaaa gctgtccggc
      661 atcggttttt ccttgctgtt cagcaatcgg catagagcga tcatcggaaa gaacaggttc
      721 tttactgtca tttgcggaag gatcaagcgc aagaagcgcc gctctggctt tcgctgattg
      781 aggagtaacc tcaagaagtc gaagataggt ttctcttgcc ccctcaatat cgcccttccc
      841 tatcctggct tccgcaagcc ctaaaaacgc atcttctccc tccggaataa gatcgagagc
      901 acgaagaaaa tgcgcttccg cttccagaaa ttctccggtt acaagaaaag cggctccaag
      961 tcgaatttgc tcttccgaca gagcggtccg ctgacggcgc tcctcatccg caataagaac
     1021 ctttttttcg gtcctttcga cagcctcagc aaagtcatct ctggtagcat ctacttccaa
     1081 ggccttgcga tacgattcga gggcttgaga aaaatcctct cttgattcca aaagggaacc
     1141 aagccgaaaa tgagtttccg aatcctccgg tgcaaatctg acgctctctt gatagttcct
     1201 aatcgcgttg tccaaggaac caagttcgaa aagcaaatcg cccagacgcc tatatacctc
     1261 cgcatcctcg ggagcgatct tcagacagct gttccatgcg tgaacggctt ctccctttct
     1321 atccagctct tcaagagttc gcgctttgcc aaagaggggt tccgcacgaa gagggttttt
     1381 ctccgccgct ttttcgtaaa gaagcaacgc cctgtcgtac tctttttgcg cgtaggcttg
     1441 gtcgccttgc tgcaatatct tttcaaacga ttctccggaa gcaaaaaaaa gtccgcccat
     1501 tacaagcgct gccgccagca aacaaatcgg caacactcgg ttttttgtgt ttttctcacg
     1561 ctgcggctgg gcgtgagtcc ggttcgtttc gacaaaaaac gttttttcag gggcagaatc
     1621 ctcttcaatc aaaggataga gcggatgggc cggaattgtg aacgtagaaa cggcgctgcg
     1681 gctctcgccg ctctcttcct gaacagaaac ggaaacaccg gtttcatccg gttcaatgga
     1741 aggagcaagc ggctcttccg tt
//
LOCUS       DAOSWW010000623         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57721_length_1762_cov_3.159930, whole genome shotgun sequence.
ACCESSION   DAOSWW010000623 DAOSWW010000000
VERSION     DAOSWW010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57721_length_1762_cov_3.159930"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..692
                     /locus_tag="PK442_11230"
     CDS             6..692
                     /locus_tag="PK442_11230"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO88269.1"
                     /translation="MENGYYEKKLNAEKLYQVYQTAIPQVLDYLRSEIEFVRERLRGD
                     EAALELGAGYGRIMKELSPSVRKITGIDVSEQSVALGREYLGDLANCTIEMMDAHTLA
                     FDETFDVVLCLQNGLSAMKGDPENLVRRSLRVLAPGGTAFFSSYSPKFWEHRLAWFQE
                     QAGKGLLGEIDMENTGNGRIVCKDGFTATTFDREDMERLGKASGHPYRVEEVNNSSIF
                     LILRNEAPRS"
     gene            750..1649
                     /locus_tag="PK442_11235"
     CDS             750..1649
                     /locus_tag="PK442_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HOO88270.1"
                     /translation="MHRTPPAAEGFIQFNGYKIWYRVVGEREEPGKLPLLCLHGGPGA
                     PHDYLEPLEALASGGRRVFFYDQLGCGNSDRPSDPSMWTVGLFLEELGVVRKALGLDR
                     VHILGQSWGGMLAMEYALTRPSGLASMIVADSPASMLQWVSEANILRAALPPETQRTL
                     LEHEAAGTTDAPEYQEAMNVFYKRHLCRLDPWPECLLRAFEKTGFEVYTSMNGPSEFH
                     VTGKLKEWDIVERLPEIDTPTLVIGGRYDEATPAITETVHRGIRGSEWVIFEESAHMP
                     HLEETERYLRVVGDFLNRIEARL"
BASE COUNT          366 a          528 c          576 g          292 t
ORIGIN      
        1 aacacgtgga gaacggctac tacgaaaaga agctgaacgc ggagaagttg taccaggtct
       61 accagacggc gattccgcag gtgctcgact accttcggtc ggagatagag ttcgttcgcg
      121 aacgtctgcg cggcgacgaa gcggcgctgg agctgggcgc gggctacggc agaatcatga
      181 aggagctctc cccctccgtt cggaagatca cgggcatcga cgtctcggag cagtccgtcg
      241 cgctcgggag agagtatctg ggagacctcg ccaactgcac gatcgaaatg atggacgcgc
      301 acaccctggc gttcgacgag acgttcgacg tcgtgctctg cctgcagaac ggactgtcgg
      361 ctatgaaggg cgatccggag aacctggtcc gccggtcgtt gcgcgtgctc gcgcccggag
      421 ggacggcctt cttcagcagc tacagcccga agttctggga gcaccgtctg gcgtggtttc
      481 aggagcaggc gggaaagggc ctgctcgggg agatcgacat ggagaacaca ggaaacggcc
      541 ggatcgtctg caaggacggc ttcaccgcga ccacgttcga ccgcgaagat atggaacgcc
      601 tggggaaagc ctcggggcat ccctacaggg tagaggaagt gaataactcg agcatcttcc
      661 tgatactgcg caacgaggct ccgcgctcgt gaagcgcacc gcgcctcatg cgagcgatag
      721 cgatcatcat gaaataggag ggggtacgaa tgcaccggac accgccggcc gcagagggat
      781 tcatccagtt caatgggtat aaaatctggt accgcgtcgt cggggagcgc gaggagccgg
      841 ggaaactgcc gcttctctgt ctgcacggcg gccccggcgc gccgcacgac tatctcgaac
      901 cactggaggc gctggcctca ggcggacgga gagtgttttt ctacgatcag ctggggtgcg
      961 gcaattcgga ccgcccgagc gacccgtcga tgtggacggt cggtctgttt ctcgaagaac
     1021 tcggcgtcgt ccggaaagcc ctcggactgg atcgcgtcca catcctggga cagtcctggg
     1081 gaggcatgct cgcgatggaa tacgccctca cgcggccgtc cggactggcg agcatgatcg
     1141 tcgccgactc ccccgccagc atgcttcagt gggtttccga ggcgaacatc ctgagagcgg
     1201 cgcttccccc cgaaacgcag cggacgcttc tggagcacga agccgccgga accacggacg
     1261 ctcccgaata ccaggaggcg atgaacgtct tctacaaacg ccacctctgc cgcctcgacc
     1321 cgtggcccga gtgcctcctc agagcgttcg agaagacggg cttcgaagtc tacacctcca
     1381 tgaacgggcc gagcgagttc cacgtcaccg gcaaactcaa ggagtgggac atcgtggagc
     1441 gccttccgga gatagacacg cccacgctcg tcatcggcgg ccgctacgac gaggctaccc
     1501 ccgccattac cgaaacggtg catcgcggca tacgcggctc cgaatgggtg atcttcgagg
     1561 agagcgcgca catgccgcac cttgaagaga cggagcgcta cctgcgcgtg gtcggcgact
     1621 ttttgaacag gatcgaggcg cgcctgtagt cgcgcagcat actcaaggag ggaacatcat
     1681 gcgtgaggaa atgagctgca aagagcagga caagacgctc gccgcggtgt gcggcctgta
     1741 ctgcgaggcg tgctcgcttt tt
//
LOCUS       DAOSWW010000624         1761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57754_length_1761_cov_7.099062, whole genome shotgun sequence.
ACCESSION   DAOSWW010000624 DAOSWW010000000
VERSION     DAOSWW010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57754_length_1761_cov_7.099062"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PK442_11240"
     CDS             <1..381
                     /locus_tag="PK442_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOO88271.1"
                     /translation="ADIRRKQQICDVFLSGTNALTMDGRLVNMDGSGNRVAAMIFGPG
                     KVIVVAGTNKIVKDLAAAEERIQMKAAPINNKRLGTPNPCSRTGVCMDCQGPTRICNV
                     LTIISKRPLGTNFHVLIVGEELGF"
     gene            complement(482..1033)
                     /locus_tag="PK442_11245"
     CDS             complement(482..1033)
                     /locus_tag="PK442_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HOO88272.1"
                     /translation="MLESLVTSKTRARLLLKFFLNPNTSAYLRGLAEEFGESTNGVRV
                     ELNRLSEAGLLECADEGRTKVYRANTRHPLFPELQRIAAKTLGIDQVVEHVVSRLGNV
                     ELALVTGDYAKGIDSGLIDLVLVGRIDKMYFESLAEKIEGIMQRKIRPLFLTREEFLR
                     LKERIISENALLLWGDESEIAAK"
     gene            complement(1340..1455)
                     /gene="rrf"
                     /locus_tag="PK442_11250"
     rRNA            complement(1340..1455)
                     /gene="rrf"
                     /locus_tag="PK442_11250"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1520..>1761)
                     /locus_tag="PK442_11255"
     rRNA            complement(1520..>1761)
                     /locus_tag="PK442_11255"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          386 a          545 c          472 g          358 t
ORIGIN      
        1 gccgatatcc gcaggaagca gcagatctgc gacgtcttcc tctccggcac gaacgccctc
       61 accatggacg gcagactcgt caacatggac ggctccggaa acagggtcgc cgcgatgatc
      121 ttcggccccg gcaaggtcat cgtcgtcgcg ggaacgaaca agatcgtcaa ggatctggcc
      181 gcagccgagg agcgcatcca gatgaaagcc gcccctatca acaacaagcg gctgggcact
      241 cccaacccct gttcgcggac cggcgtctgc atggactgcc agggaccgac caggatctgc
      301 aacgtcctca ccatcatcag caaacgcccg ctcgggacca acttccacgt gctgatcgtc
      361 ggcgaagagc tgggattcta gaacgcctcg cacctcgcaa cagggggaga cgatgcggaa
      421 atgctcgtct ccccctgttc ttttcaatct ttcccggaaa tccgcggaat cggaaatcgg
      481 cctacttcgc cgcgatctcg ctctcgtctc cccacagaag cagcgcgttc tcggaaatga
      541 tgcgctcctt gagacgaagg aactcctctc tcgtgaggaa gagggggcgt atcttccgct
      601 gcatgatccc ctcgatcttc tccgcaaggc tctcaaaata catcttgtcg atccgcccaa
      661 caagcacgag atcgataagt cctgaatcga tccccttggc gtagtcgccc gtcacgaggg
      721 cgagctccac attgccgagg cgggagacga cgtgctccac aacctggtcg atcccgagcg
      781 tcttggccgc gatacgctgc agctccggaa agagagggtg gcgcgtattc gcacggtaga
      841 ccttggtacg cccctcgtcg gcgcattcaa gaagccccgc ctccgagagg cggttcagtt
      901 cgacgcgtac gccgttcgtc gactcgccga actcctcggc aagcccccgg agatatgcgc
      961 tggtgttggg attcagaaaa aacttcaaaa ggagcctggc gcgggtcttc gatgtcacaa
     1021 gagattcaag catgcttcgt ccttttttgt gatgtattta gagtaatttt attgctcgaa
     1081 agggggattg tcaaacgctg gaaagctgat tcgcaaaagc gaagcaattc cggaaagatg
     1141 agcgcgcgca catcacggcg aacgtctcca aacacgaaga gccgcccctt gaggatttcc
     1201 tccggggcgg ctcttcgtca tgtgaaagtc ccggagaacg tcgttgtcgt atactcacgc
     1261 ggtcaataga cggcatatac acaggaagcc gtcccgaggg ttttcccctg gacggcttcc
     1321 tgtgttcttt caaaaggatt tccggcggcg acctgctctc ccacggatag cctccgcagt
     1381 accatcggcg ctggagggct tgactgccgg gttcggcatg gaaccgggta tgacacctcc
     1441 gctgtgacca ccgaaaatcc aatctataca cccgacggat tttacagctt catagtttag
     1501 aaagaatctc cgaaaaagag aggaaaaggc ctcggcgtat tagtaccggt cagctcaacc
     1561 cctcgcaggg ctcacacctc cggcctatct atcccgtagt ctccgggacg ccttacttcc
     1621 ttacgaaacg gggtatctca tcttgaagtc ggcttcccgc ttagatgcct tcagcggtta
     1681 tccggtccga acatagctac ccggcgtatg caactggcgt cacaaccggt acaccagcgg
     1741 ttcgtccact tcggtcctct c
//
LOCUS       DAOSWW010000625         1760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57835_length_1760_cov_3.331378, whole genome shotgun sequence.
ACCESSION   DAOSWW010000625 DAOSWW010000000
VERSION     DAOSWW010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57835_length_1760_cov_3.331378"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PK442_11260"
     CDS             <1..1093
                     /locus_tag="PK442_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOO88273.1"
                     /translation="FDRDPYLVVSEGRLVWMQDAYTATDSYPYSQPVSLGRGQNINYI
                     RNSVKATVDAFDGTMRFYAADPDDPVLKAWSGIFPEIFKPYSEMPKGLKAHLRYPQDL
                     FNIQSEIYRTYHMTDANTFYNKEDVWDRPGSADSSGSAADAYYVNMRLAGEKHAEFAL
                     ISPFMPVSRNNMIAWMAGRSDGDNYGQLLVYQFPKQKLIYGPAQVEALTNQNSEISAQ
                     LSLWSQRGSSVVRGNMTVVPVGNGLLYVQPLYLRAENSELPELRRVITSTGGRVVWGE
                     TLEESLVRLLSPTASELETSSLVPAETTERTEFSGSEALAELAAEAMTHWDASQEALR
                     KGDWEGYGRGMSGLENVLREMMDRKDREQ"
     gene            1223..>1760
                     /locus_tag="PK442_11265"
     CDS             1223..>1760
                     /locus_tag="PK442_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOO88274.1"
                     /translation="MSLLIRRSITALFLFSALLFLLIGIEGEKIRFMIADMEIFRPPN
                     LGSTEGFWSVNPEMIELFQNAGRTIPSSFSDIARTFTSAGHVAATLFEATAENIRNLA
                     VFITEQNANIPMESALIQAAAFWKYSIKYEVPLDLIVAVAHTESHFNPEARSGAGAAG
                     VMQVMWKVHSGLLQANGIL"
BASE COUNT          417 a          507 c          503 g          333 t
ORIGIN      
        1 cttcgacagg gatccgtacc ttgtcgtctc ggaaggacgc ctcgtctgga tgcaggacgc
       61 ctatacggcc acggacagct acccctactc gcagcctgtc tcgctcggac ggggacagaa
      121 catcaactac atacgcaaca gcgtaaaagc cacagtcgac gccttcgacg gtacgatgcg
      181 attctacgcc gccgaccccg acgacccggt gctgaaggcg tggagcggca tcttcccgga
      241 gatattcaaa ccgtactcgg aaatgccgaa ggggctgaaa gcgcacctgc gctacccgca
      301 ggacctcttc aacatccaga gcgagatcta ccgcacgtac cacatgacgg acgcgaacac
      361 tttctacaac aaagaggacg tgtgggacag gcccggaagc gccgattcaa gcgggtcggc
      421 agctgatgca tactacgtga acatgcgtct cgcaggagag aaacatgcgg agttcgccct
      481 catttctccg ttcatgccgg tgagcaggaa caacatgatc gcgtggatgg cgggccgatc
      541 ggacggggac aactacggcc agttacttgt ctaccagttc ccgaagcaga agctcatcta
      601 cggccccgcg caggtagaag cgctcacgaa ccagaattcg gagatctccg cacagctctc
      661 cctctggagc caacgaggct cgtccgtcgt caggggcaac atgacggtgg tgccggtggg
      721 gaacggactt ctctacgtcc agccgctcta cctcagggcc gagaacagcg aacttcccga
      781 gctacggcgc gtgatcacat cgacaggagg ccgggtggtc tggggagaaa cgctcgagga
      841 atcgctcgtt cgcctgctct ctcccacggc gtcggaactg gaaacatcct cccttgttcc
      901 ggctgaaaca acggagcgga cggagttctc aggatcggaa gcgctcgccg aacttgcagc
      961 cgaagcgatg acgcactggg acgcctctca ggaagcattg cggaaaggcg actgggaggg
     1021 gtacggcagg gggatgagcg gactagaaaa cgtactccgt gagatgatgg accggaaaga
     1081 ccgggagcag tagctcagct ggcgccgtat tgtaaagaaa atacacgcat tcagactctt
     1141 gagagtgcta taataatgcg accttccatc ggcccttcct ttggaaggtc gcatttcttt
     1201 gaataagagg gaggggaacg aaatgtcgct cttgatacgg agaagcatca ccgcactttt
     1261 tcttttttcg gcattgctct ttcttctcat tggaatagaa ggggaaaaaa tccggttcat
     1321 gatcgcggac atggagatat tcagaccgcc caaccttggg agtactgaag gtttctggtc
     1381 ggtcaacccc gagatgatcg agctctttca aaatgccgga cggacaattc catcgtcgtt
     1441 ctcggatatc gccaggacat tcacctcggc agggcacgtc gccgccacgc tcttcgaagc
     1501 gacagccgaa aacatacgga atcttgccgt ttttatcacg gaacagaacg cgaacattcc
     1561 catggaatcc gcactgatac aggccgcagc cttttggaaa tactccatta aatacgaggt
     1621 tcccctcgat ctgatcgtcg ccgtcgcgca cacggagagc cacttcaacc cggaagcccg
     1681 tagcggcgcc ggcgcagcgg gcgtcatgca ggtgatgtgg aaggtgcact ccggcctgct
     1741 gcaagccaac gggattctct
//
LOCUS       DAOSWW010000626         1759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57872_length_1759_cov_4.044601, whole genome shotgun sequence.
ACCESSION   DAOSWW010000626 DAOSWW010000000
VERSION     DAOSWW010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57872_length_1759_cov_4.044601"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..994
                     /locus_tag="PK442_11270"
     CDS             23..994
                     /locus_tag="PK442_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOO88275.1"
                     /translation="MYYLNAESMISSFSFSGVMDAVANAMIQIETSPNSLIAPLRNSV
                     SLEKNTLMMMPCLSEKEWGLKVLTLFPDNPVRLKPFINGLFLLFDGSDGSPSALLDGR
                     TLTAIRTGGVGGLAVRTLASTHATRLGIVGAGEQSYWQVRFACSVRPFSQVILYDTIE
                     SKAELCAERIRKVLPELEIEITKDTTFLLKHSDVVITATNTTTPVFEDSPMYFEGKTF
                     VGIGSYRPDMREYPDALFRAISNIFVDTEHAFDETGDLIEPLQSGVVSKKNIRSMGAF
                     LSREKADFTAESGAFFKSVGYAAFDLFVARFLFQRGVENGKAIDLGI"
     gene            1037..1516
                     /locus_tag="PK442_11275"
     CDS             1037..1516
                     /locus_tag="PK442_11275"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HOO88276.1"
                     /translation="MREEDETLLRKSVDAMARALALYHGIFAREAVGMLGQEKGTALV
                     ENVVRQFGLERGRRVREQALAAGDALDLKGMNEHYDLPLAYAWDALKQENGSDVSFCP
                     MADEWKRNGMVEEGKLYCAVDYAIAEGFSPDLHFSRSSSLMHEDSCCCHRYSNNLEK"
     gene            1527..>1759
                     /locus_tag="PK442_11280"
     CDS             1527..>1759
                     /locus_tag="PK442_11280"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOO88277.1"
                     /translation="MKKFGRLISLLLLLLIAEILYSSLKSYFFKSPPIWSFEVSLFLY
                     GSFFMLGAAYCHMEKKHVSVDVLNHYLSPKWKR"
BASE COUNT          431 a          366 c          449 g          513 t
ORIGIN      
        1 ctaacaaagg gggttgtatg ttatgtatta cttgaatgcg gaaagcatga tttcttcttt
       61 ttctttctca ggtgtaatgg acgctgttgc caatgccatg attcagattg aaacatcccc
      121 aaacagcctt atcgctcctc tccgtaattc tgtctctctt gagaaaaata cactgatgat
      181 gatgccctgt ctttctgaga aagagtgggg actgaaggtg ttgacactct ttcccgataa
      241 tccggtacga ctcaaaccat ttattaacgg tctttttctt cttttcgacg gttcggatgg
      301 cagtccctcc gctcttcttg acggaaggac tttaaccgcg attcggactg gaggggtagg
      361 aggcttggct gttcgcaccc tcgcttctac tcatgcaacc cgcttgggaa tcgtcggcgc
      421 gggggaacaa agctattggc aggtccgttt tgcgtgttcc gttcgtcctt tttcccaagt
      481 gattctttat gatacaatcg aatcaaaggc ggagctttgt gccgaacgca tacgtaaagt
      541 cctccctgaa ttggagatcg agattacgaa agatacaaca tttttgttaa aacactcgga
      601 tgtggtaata actgcgacca ataccacgac tcctgttttt gaggatagtc cgatgtattt
      661 cgaaggaaaa acttttgtcg gtataggttc gtaccgtccg gatatgagag agtatccgga
      721 cgcgcttttc agagcaattt caaacatttt tgtagatact gagcatgctt tcgacgagac
      781 tggcgacctt attgaacctt tacagtcagg tgttgtttca aagaaaaata tccgttcgat
      841 gggagctttt ctatcccgtg aaaaagccga ctttaccgct gagtccggag cgtttttcaa
      901 atccgtcggc tatgctgctt tcgatctttt cgtagcgcgg tttcttttcc agcggggcgt
      961 agagaatgga aaggctatag acttgggaat ataagcaggc atccggggaa acactcggaa
     1021 agaaggtggg gaggatatga gagaagaaga tgaaactcta ttgagaaaga gcgtcgatgc
     1081 gatggcccgc gcattggctc tctaccatgg catttttgcc cgtgaggcgg tagggatgct
     1141 ggggcaggag aaagggaccg ctcttgttga gaatgttgtg cgtcagttcg gtcttgaacg
     1201 tgggcggcgt gtcagggaac aagcgctcgc tgcgggtgat gcgctggatt tgaaaggaat
     1261 gaatgaacac tacgatttgc ctcttgcgta tgcatgggac gcattgaagc aggaaaatgg
     1321 aagcgatgtc tctttttgtc cgatggccga cgaatggaag cgaaacggta tggtggagga
     1381 aggaaaattg tattgcgctg tagattatgc cattgccgaa ggcttctctc ctgatttgca
     1441 tttttcaaga tcctccagtt tgatgcacga agattcctgt tgttgccatc gctactccaa
     1501 caaccttgaa aagtgaggtg agcgttatga aaaaattcgg tagacttatc tctctccttc
     1561 tcttgcttct tattgcagaa atcctgtaca gttcattaaa atcatatttt ttcaaatcac
     1621 ctccgatatg gagctttgaa gtatccctct ttttgtacgg gtcgtttttt atgttgggag
     1681 cggcatattg tcacatggag aaaaaacatg tttccgtcga tgtgctgaac cattacctct
     1741 cgccaaaatg gaagaggat
//
LOCUS       DAOSWW010000627         1757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_57987_length_1757_cov_2.929495, whole genome shotgun sequence.
ACCESSION   DAOSWW010000627 DAOSWW010000000
VERSION     DAOSWW010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57987_length_1757_cov_2.929495"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..334)
                     /locus_tag="PK442_11285"
     CDS             complement(134..334)
                     /locus_tag="PK442_11285"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOO88278.1"
                     /translation="MTEFYAVIDRDEDGQFFAYVPELPGCHTCGETMEELLENLEEAI
                     ALYLEVVEHPREFPSIRKVAVG"
     gene            602..832
                     /locus_tag="PK442_11290"
     CDS             602..832
                     /locus_tag="PK442_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88279.1"
                     /translation="MPRATLEISAKKELYRRFRKLSERNAVRVLGYIDALEEERPNEE
                     TVAALEEGERMLKDPGALRFSTVEDLLTELRS"
     gene            829..1116
                     /locus_tag="PK442_11295"
     CDS             829..1116
                     /locus_tag="PK442_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HOO88280.1"
                     /translation="MTLRYEIQRTSRFKRDFALVERRGCDISLLADVIRALASGETLP
                     RQERDHTLSGDYAEYRECHIRPDWLLIYKITEESLVLTLERTGTHSDLFKK"
     gene            complement(1106..1432)
                     /locus_tag="PK442_11300"
     CDS             complement(1106..1432)
                     /locus_tag="PK442_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88281.1"
                     /translation="MEAATPYETILYLARKYIWWKTPEEAAAMPERVLAQVMNIGDYD
                     DVLLMKSRFEDDALRNVIAHAEAGQFNARSWHYWHYMLGLAEIDHVPPLPTRRFSWRG
                     EEKTTS"
BASE COUNT          386 a          495 c          511 g          365 t
ORIGIN      
        1 cagatggtgc actatatgaa gctgaaggac cgcggctccc tcggcgtccg cggcatcagc
       61 aacaaagggg acgtcgtgtc caccggagcg ctggagaagg cgcgggatat cgggaagagc
      121 atctgagggg ggctcatccg acggctactt tccggatgct gggaaattcg cggggatgct
      181 ccacaacctc gaggtacagc gcgatcgcct cttccagatt ctccagaagc tcctccatgg
      241 tctcgccgca cgtatggcag cccggaagct ccgggacgta ggcgaaaaat tgaccgtcct
      301 cgtctctgtc tatcaccgca tagaattcgg tcattgcgat acctccttga gacctcgatt
      361 caagagaagc gcgtcgaccg attgatggag gtcgacttta cgagaacaat tctatatgtt
      421 gcgggggttt tttgcacgcg gagattcctg tgaagtcgta ctgccggaag ttgcgattgc
      481 cttttatagc gggaaagcag gcaggagtac cgccggctga cgcagtaacg cggaggtctc
      541 cccggcgttg aagcgtgccg gaattcgctg tacaatactc ctacgcgaag gggggatacc
      601 aatgcctcgg gctacacttg aaatttccgc caaaaaagaa ctgtatcgcc gtttccgcaa
      661 actctcggag cggaatgccg tgcgggtgct cggctacatc gacgccctcg aggaggagcg
      721 ccccaacgaa gagaccgtag ccgcgctgga agaaggcgag cggatgctga aagaccccgg
      781 cgcgttgcga ttttcgaccg tcgaagacct tctgaccgaa ctgaggtcat gacgctccga
      841 tacgaaatcc agagaacctc gcggttcaag agggatttcg cgttggtcga aagaagggga
      901 tgcgatatct cccttctcgc cgatgtcata cgagcattgg caagcgggga gactcttccg
      961 aggcaagaga gggatcatac attgtccggg gactacgcgg agtatagaga atgccacata
     1021 cgtcccgact ggcttctcat ctacaagatc acggaagaat cgctcgttct gacactggag
     1081 cgcaccggaa cccactccga cctcttcaag aagtagtttt ctcctcccct ctccacgaaa
     1141 agcgtcttgt cgggagcgga gggacgtggt cgatctctgc gagcccgagc atgtaatgcc
     1201 agtagtgcca tgacctggcg ttgaactgcc ctgcttcggc gtgcgcgatc acgttgcgga
     1261 gcgcatcgtc ctcgaagcgg gatttcatca gaagcacgtc gtcatagtcc ccgatgttca
     1321 tcacctgggc cagaacacgc tcaggcatgg cggcggcttc ttcaggagtc ttccaccaga
     1381 tgtatttgcg ggcgagatag agtatggttt cgtacggtgt cgcggcctcc atgaaaacac
     1441 ctcctgtcct gagttgcacg caacctctcc caagcctgcg caggaaccgc ttttaaaaca
     1501 ttctactgcg agccgttctt ttatgcgtgt gccgaacacc tctattttat acgccggagg
     1561 ttgtttttgc acgcgaagct gccgatgaga cgacgtgcgt tgagcgcctg cgtcgtcctc
     1621 ttatgtcgcg cacaacccca acgcgctcgc cgcacataaa agagccggcg aagagtgccc
     1681 ctcctcccga taaggcgctc tggaccggac ggggaagagg aagtacactt atgcggaatt
     1741 cgccggatag ttccggc
//
LOCUS       DAOSWW010000628         1756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_58042_length_1756_cov_3.111111, whole genome shotgun sequence.
ACCESSION   DAOSWW010000628 DAOSWW010000000
VERSION     DAOSWW010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58042_length_1756_cov_3.111111"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..1277)
                     /locus_tag="PK442_11305"
     CDS             complement(45..1277)
                     /locus_tag="PK442_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HOO88282.1"
                     /translation="MGYREDGKWWVSPANYEAEVTKTFSFAPKIEILDTTLRDGEQQP
                     GIIFTKEDKVAIAKKLDEVGVHRIEAGTPAGSQEDADAIKAICDLGLKSKIFAFCRAM
                     PKDMELAKSLGVDGVLCEMIGSEQMLRYGKRWTPEQAIAACIEATQAAHELGMFVTFF
                     PADGSRAEMNYLLDFVQAVAEGGHIDSLALVDTFGTFSPEGAVYMLKKLKERFSFPIE
                     AHFHSDYDIGVATTLAALKEGASVAHVTVNGLGERAGSCPLEPLALSLEALYGQSTGI
                     VLNKLTELSKLVEELSRFPVPPIKPVVGNKLFGWETGLPSSLWTNAKTENPLIMLPYH
                     YSLTGREEPVLYIGKKSGKDNVKYWLAKTGLSLDDEGEKTLLQKVKDLSISLKRDLNE
                     DEFRGLVSRVKEESACNQ"
     gene            complement(1355..>1756)
                     /locus_tag="PK442_11310"
     CDS             complement(1355..>1756)
                     /locus_tag="PK442_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOO88283.1"
                     /translation="LARFPGARGVLVQEQIGKGIELILGADSDPLLGHAVLVGWGGTG
                     VEVFSDVALGHVPFSRAEGMRLLERLQCFPLLKGYRGKPGVDLEGLLDLLQKLQRLLL
                     DFPEIEEMDLNPLIWDSKRFIAADYRIKTRS"
BASE COUNT          302 a          559 c          514 g          381 t
ORIGIN      
        1 cagcatcccc gacggctgga tgttctgaaa gcgttgcgct attttcactg gttgcatgca
       61 ctctcctcct tcacccggga gaccagcccc cggaactcgt cttcgttgag gtcgcgcttc
      121 agagagatgg agagatcctt caccttctgg agcagcgtct tctctccctc atcatccaac
      181 gaaagtccgg tttttgcaag ccagtatttt acgttgtcct tgccgctctt tttgccgatg
      241 tagagcaccg gctcctcccg tccagtcagc gagtagtggt aggggagcat gatgagcggg
      301 ttttccgtct tcgcgtttgt ccagaggctc gacggaagtc cggtttccca gccgaagagc
      361 ttgttgccga cgaccggttt gatgggcggc acggggaagc gggaaagctc ctccaccagt
      421 ttcgagagtt ccgtgagctt gttgaggacg attccggtgg actggccgta gagcgcctca
      481 agggagagcg ccagcggctc gagcgggcag cttcccgcgc gctcgccgag accgttcacc
      541 gtcacgtggg cgaccgacgc gccctctttg agcgcggcga gcgtcgtcgc cacgccgatg
      601 tcgtagtcgc tgtggaaatg cgcctcgatc gggaaggaga aacgctcttt gagtttcttg
      661 agcatgtaga ccgctccttc gggagagaat gtgccgaagg tgtcaacgag cgccagcgag
      721 tcgatgtgcc cgccctcggc gaccgcctgc acgaagtcga gcaggtagtt catctcggcg
      781 cgcgagccgt ctgcggggaa gaatgtgacg aacatcccca gctcgtgggc ggcctgcgtc
      841 gcctcgatgc acgcggcgat ggcctgctcc ggcgtccagc gcttcccgta gcgcagcatc
      901 tgctcgctgc cgatcatctc gcagagcacg ccgtccacgc cgagcgactt cgccaactcc
      961 atgtccttcg gcatcgcacg gcagaaggcg aagatcttcg acttcaatcc aaggtcgcag
     1021 atggccttga tcgcgtcggc gtcctcctgg gatcccgcgg gcgttcctgc ctcgatcctg
     1081 tggacgccca cctcgtcaag cttcttcgcg atggcgacct tgtcctcctt cgtgaagatg
     1141 atccccggct gctgctcgcc gtcgcggagc gtcgtgtcaa gtatttcgat cttcggtgcg
     1201 aaagagaatg tcttcgtcac ttccgcctcg taattcgcgg gtgaaaccca ccacttgccg
     1261 tcttctctgt atcccattct caattcctcc tcgaaagtag tgttcttctt tgcgtccggg
     1321 cggcgtgggt gtccccgccg tccggttctt cgtgtcaaga gcgtgttttt attctgtagt
     1381 ccgccgcaat aaaacgtttg ctgtcccaga taagcggatt gaggtccatc tcctcgatct
     1441 cgggaaaatc caacagcagt cgctgaagct tttgcaacag gtcgagcagc ccttccaggt
     1501 cgacgccggg cttgccccgg tagcccttca gcagcgggaa gcactggagg cgttcgagaa
     1561 ggcgcatccc ctcggcccgc gaaaagggaa cgtgccccag cgcaacgtcc gagaagacct
     1621 cgaccccggt cccgccccat ccgacgagca cggcgtgccc cagaagcggg tcgctgtccg
     1681 cgccgaggat gagttcaatc ccttttccta tctgttcctg caccagtacc ccacgagctc
     1741 ccggaaaacg ggcgag
//
LOCUS       DAOSWW010000629         1753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_58217_length_1753_cov_2.722026, whole genome shotgun sequence.
ACCESSION   DAOSWW010000629 DAOSWW010000000
VERSION     DAOSWW010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58217_length_1753_cov_2.722026"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1753)
                     /locus_tag="PK442_11315"
     CDS             complement(<1..>1753)
                     /locus_tag="PK442_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOO88284.1"
                     /translation="TNLLSEKTNPIFVPLPSDSVSPRNKVPWVMLNQTGDVVGPLGGM
                     SGVKLLERFAQLASRIQVVRFDELPIPFAAVATDLETGEKVVLRSGSLASAMRASMAI
                     PALFDPWPMGDRLLVDGGLVSNLPVETAKELFPGYPVIAVDVTGKLKTKKQIRSLVDV
                     IDQSITIFTHRNVQHESPMADLLISPPVEGVSIFDLSNAEVVIEGGKQATLAMLQAIR
                     KLAESAPPVAQRETLHLDTRVASVKVEGATLELASSIVKKYGSWVGKPLSDIEVLRAS
                     REISERDDILSVDYRFEEAGPNVDVVFAVQRKPALEINFGGYTTNLHPHRWLFVSGIR
                     RDLFKDADALKFNIKVGEQWGVDASYFDSPGKTPWEIRLSAYNWELETANAGKRSWDR
                     YALGVLKHFNGRSFNGGFGYAFERIHTGGDEFDASGPTLFLASDTLNDPVDPTDGALL
                     YMQAWWPDFDEPLFRLSYFRASRVANDWRFYFRAGFAEGDMKREGRAVYLGASEELYS
                     YSGNPIEAERMAWWNIAFRRILMKSWWGALNTEFFGGMGFAFDDEWDRFRDVWEAGVS
                     FSIPGVLFDGKLMFIYND"
BASE COUNT          370 a          526 c          499 g          358 t
ORIGIN      
        1 gtcattgtag atgaacatga gttttccgtc gaagagcact cccggtattg aaaaggaaac
       61 tcccgcctcc cacacgtcgc ggaaccggtc ccattcgtcg tcgaaggcaa atcccatgcc
      121 gccgaagaac tccgtgttca gggctcccca ccagctcttc ataaggatac gccggaaggc
      181 gatgttccac catgccatcc gctccgcttc gatcggattg cctgaatagc tatagagttc
      241 ttcggaggct ccgagataga ccgcccgtcc ctctctcttc atgtcgccct cggcaaatcc
      301 cgctctgaag tagaatcgcc agtcgttggc cacgcgcgag gccctgaagt acgagagccg
      361 gaagagcggt tcgtcgaagt ccggccacca cgcctgcata tagagaagcg ccccgtcggt
      421 cgggtcgacc ggatcgttga gcgtatcgga agccaggaag agagtgggac ctgacgcgtc
      481 aaactcgtca cccccggtat ggattcgttc gaatgcgtat ccgaaacctc cgttgaaaga
      541 gcggccgtta aagtgtttga gaacgccgag agcataccgg tcccatgagc gtttgcctgc
      601 attcgccgtt tcaagctccc agttgtaggc ggagagccga atttcccacg gcgtcttgcc
      661 cggcgagtcg aagtagctcg cgtcgacgcc ccactgttcg ccaaccttaa tattgaactt
      721 cagagcgtcc gcgtccttga agagatcccg gcgaataccg ctgacgaaga gccagcgatg
      781 ggggtggaga ttcgtcgtgt accctccgaa attgatctca agcgcgggtt ttctctgtac
      841 agcgaagacg acgtccacat tcggtccggc ctcctcgaaa cggtagtcca cggagaggat
      901 atcgtcgcgt tcgcttatct ccctgctcgc ccgcaaaacc tcgatatcgc tcagcggttt
      961 gcccacccat gagccgtact ttttcacgat ggaggatgcg agctcgagcg tagctccctc
     1021 taccttgaca gaggctacgc gcgtgtcgag gtgaagcgtt tcccgctgcg ccacaggagg
     1081 agcggactcg gcaagttttc tgattgcctg gagcatggcg agcgtcgcct gcttgccgcc
     1141 ttcgatgacc acctccgcgt tcgaaaggtc gaaaatgctc acgccctcta ccgggggaga
     1201 aatgaggaga tccgccatcg gactctcatg ctggacgttc ctgtgcgtaa agatcgtaat
     1261 tgactggtcg atcacatcca caagcgagcg aatctgtttt ttcgtcttta gttttcctgt
     1321 cacgtccaca gcaatcaccg ggtaccccgg aaaaagctcc tttgcggtct cgacgggaag
     1381 attcgagacc agaccgccgt cgaccagcag tctgtcgccc atgggccacg ggtcgaagag
     1441 cgcgggaata gccatagagg cgcgcatcgc cgatgcaagg cttcctgagc gaaggacgac
     1501 cttctctccg gtttccagat cggtggcgac tgcggcgaat ggaatcggca gctcgtcgaa
     1561 acgaaccacc tggatacggg aagcaagctg tgcgaagcgt tcgaggagct tgacccccga
     1621 catgcccccg agaggcccca caacgtcccc cgtctggttc agcatgaccc acggcacctt
     1681 gttcctggga gagacgctgt ccgaaggcaa ggggacaaag atcgggttgg tcttctcgga
     1741 gaggagattc gtg
//
LOCUS       DAOSWW010000630         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_58422_length_1749_cov_3.935065, whole genome shotgun sequence.
ACCESSION   DAOSWW010000630 DAOSWW010000000
VERSION     DAOSWW010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58422_length_1749_cov_3.935065"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PK442_11320"
     CDS             <1..1215
                     /locus_tag="PK442_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOO88285.1"
                     /translation="PGLPKIPFLLLAAFLCLMGYWVYREGKIQEEAGGKKREGRKEAA
                     PGAPGGEAPKPAPSSPEDVMRLLTVDPMEAEIGYALIPLVDPSQGGDMLDRIGTIRKQ
                     MAMELGIVVPPIRIRDNIQIKPTEYILRVKGAETGRGELLPDHYLAMNTGGAEEELVG
                     VPTTEPSFGLPAVWIPPEMRDKAESLGYTVVDAPSVLATHLSETIKRYGADLLTRQEV
                     QKLTDMVKEAAPAVVEEMTAALSLGEIQKVLQNLVREQVPVRDLVSIFESLADYGKVS
                     RSVDFLTERVREALARLITLKIQDESGVVTVATLSPNWEQKVKEAVDGDLMRGWQFNM
                     DPREVQKMMAAVARAVEEMSMKGHLPVLLVHPDVRLIVRRILEASLPTLFVISYNEIA
                     QGVQLKSVGMVE"
     gene            1216..>1749
                     /locus_tag="PK442_11325"
     CDS             1216..>1749
                     /locus_tag="PK442_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88286.1"
                     /translation="MAMRVRSQISFEANDEAEAFRTARDRLGPDAAILSQRIVKTGGF
                     LGFFRRTVLQVTAGILETEENRSESDSEKKERLAAFQKLLEFKQAMAAAEEGVTTSEA
                     GLLAGRKDLSAAGVYSPLSKPPASVSQRPPEEPDRAEISARGRALASEKKVPDQEYRT
                     KLELEVSELSNRLDKVLK"
BASE COUNT          361 a          475 c          568 g          345 t
ORIGIN      
        1 cccggtcttc caaagattcc ctttctgctt ttggcagcct ttctctgcct gatggggtac
       61 tgggtctacc gcgagggaaa gatacaggag gaagcgggcg gcaagaaaag ggaaggtcga
      121 aaggaagccg ctccgggcgc tcccgggggc gaggccccga aaccggctcc ctcctctcct
      181 gaagatgtga tgcgtctgct gacggtcgat ccgatggagg cggagatagg gtatgccctc
      241 atcccgctgg tggatccttc gcagggcggg gacatgcttg accggatagg aacaatccgc
      301 aaacagatgg cgatggagct ggggatcgtc gtaccgccga tacggattcg cgataacatc
      361 cagatcaagc cgacggagta cattctccgc gtcaaggggg ccgaaactgg gcgcggagag
      421 ctgctgcccg atcactatct tgccatgaac accggaggcg cggaggagga gcttgtcggc
      481 gttcccacca ccgaaccctc tttcgggctt cctgcggtct ggattcctcc cgagatgcgc
      541 gataaggccg agtcgctggg gtacaccgtc gtcgacgcgc cttcagtatt ggcgacccac
      601 ctttcggaga cgattaaacg ctacggcgcc gatctgctga cgcggcagga ggtgcagaaa
      661 ctcaccgata tggtgaagga ggctgctccg gctgttgtcg aggagatgac cgcggcgctc
      721 tcgctcggag agatccagaa ggtgctgcag aatcttgtcc gggagcaggt ccccgtgcgc
      781 gacctggtga gtatcttcga atcgctcgcg gactacggca aggtatcccg gagcgtcgat
      841 tttctcaccg agcgcgtccg cgaggcgctc gcgcggctca tcacgctcaa aattcaggac
      901 gagtccggcg tggtcaccgt ggctaccctc tctcccaact gggaacagaa ggtgaaggag
      961 gctgtcgacg gtgatctgat gcgtgggtgg cagttcaata tggatccccg cgaggtgcag
     1021 aagatgatgg ccgctgtggc gagggcggtg gaggagatgt ccatgaaggg gcatcttccc
     1081 gtactgctgg tccacccgga cgtccggctg attgtccgcc ggattctcga agcatcgttg
     1141 ccaacactgt ttgtgatatc ctataatgaa atagctcagg gcgtacagct caaatcggtc
     1201 gggatggtgg aataaatggc gatgcgtgtg cgatctcaaa tctcctttga agcgaacgac
     1261 gaggcggagg ccttccgtac tgcccgtgat cgcctcgggc cggatgccgc aatactctcg
     1321 cagcgcatcg taaaaaccgg tggttttttg ggtttttttc gcaggactgt gcttcaggtg
     1381 accgccggaa tcctggagac ggaagaaaac cgcagcgaga gcgacagcga aaagaaagag
     1441 cggctggctg cttttcagaa gctgcttgag ttcaagcagg cgatggctgc tgcggaagag
     1501 ggcgtgacca cttcggaggc cgggctgctc gccgggcgga aagatctctc cgccgcagga
     1561 gtgtattctc ctttgtcgaa acctccggcg agcgtttcgc agcgtcctcc tgaagagcct
     1621 gaccgggcgg agatttctgc acggggacgc gcgctcgcgt cggagaaaaa agtcccggat
     1681 caggagtacc ggacgaaact tgaacttgaa gtcagcgaac tttcgaatcg tcttgacaag
     1741 gtgctcaaa
//
LOCUS       DAOSWW010000631         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_58429_length_1749_cov_3.475797, whole genome shotgun sequence.
ACCESSION   DAOSWW010000631 DAOSWW010000000
VERSION     DAOSWW010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58429_length_1749_cov_3.475797"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PK442_11330"
     CDS             complement(<1..852)
                     /locus_tag="PK442_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO88287.1"
                     /translation="MFTLLWGGLQTTFSLAIFPFLVLGVTGGIVIGALPGLTATMGVA
                     VLLPLTFGMESTRALVMLSGIYIGAIYGGSISAILLKTPGTPAAAATVLDGYELARKG
                     EAGKALSISAIASFTGGIVSTVMLITISPILARFALRFGAPEYFALAVFGLSIIASIS
                     GKSPVKGLLAGMFGLLISTVGLDPVTSFPRFTFGEINLFNGFAIIPVLIGLFALAEAF
                     VQMEKIFEPAQTVKTDFKRGMVSLKETLSLMPLMIKSALIGTFIGSIPGAGADIAAFV
                     SYNEARRS"
     gene            complement(865..1332)
                     /locus_tag="PK442_11335"
     CDS             complement(865..1332)
                     /locus_tag="PK442_11335"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOO88288.1"
                     /translation="MKKANYGIVLVFLLLSAFVIYQANNFEQTLIQDDYVGASFFPEL
                     LAWMTAGLALVLGWLNFRGKMDDDGRTLADLFPRQILLAVVGLGLVVGYVMLLEPLGF
                     ILATIALNAALLLLFGVRSSLVLALFPVIVSLSVYVVFYKILIVPLPEGLFYF"
     gene            complement(1445..>1749)
                     /locus_tag="PK442_11340"
     CDS             complement(1445..>1749)
                     /locus_tag="PK442_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOO88289.1"
                     /translation="CSDVRMAGLPDVPTMTELGYPIVTGTWRGIAVPKGTPEDVKAVL
                     GAALEKAVAEKEFVDFMNGRTLGIRFLNAADFKTFVENDVKALDAVVEEVKKQQQQ"
BASE COUNT          398 a          502 c          548 g          301 t
ORIGIN      
        1 ggagcgtcgc gcctcgttgt agctcacaaa ggcggcaatg tccgccccgg ctccgggaat
       61 ggagcctatg aacgtcccga tgagcgccga cttgatcatc agcggcatca gggagagggt
      121 ctccttcaga gagaccattc ctctcttgaa atctgtcttc acggtctgcg cgggttcgaa
      181 gatcttctcc atctggacga aagcctcggc cagcgcgaag agaccgatga gcacagggat
      241 gatggcgaag ccgttgaaga ggttgatctc gccgaaggtg aaacgcggga agctggtgac
      301 cgggtcgagc ccgactgtcg aaatcagcag gccgaacatt cccgccagga gccccttcac
      361 cggactcttt ccggagatgc tcgcgatgat gctcaggccg aagacggcga gcgcgaagta
      421 ctcgggcgcg ccgaagcgaa gtgcaaagcg ggccagtatc ggcgagatgg tgatcagcat
      481 gaccgtgctc acgataccgc cggtgaacga ggcgattgcg gagattgaga gcgctttgcc
      541 ggcctcgccc ttccgggcca gttcgtagcc gtcgagtacc gtcgcggctg cggcgggcgt
      601 ccccggagtt ttcaacagaa tcgcggagat ggagcccccg tagatggcgc cgatgtagat
      661 gcccgagagc ataaccagcg cgcgggtgga ctccatgccg aacgtgagcg gaagcagcac
      721 cgcgacgccc atggtcgcgg tgagaccggg aagcgccccg atgacgatgc ccccggtaac
      781 gccgaggacg aggaacggga agatggcgag agagaaggtg gtctgaagac cgccccagag
      841 aagagtaaac attcctgccg cctcctagaa gtagaagagc ccctccggaa ggggcacgat
      901 gaggatttta taaaacacca cgtacaccga caacgagacg atcacgggaa agagtgcaag
      961 gacgagagag ctgcgcacgc cgaagagcag aagcagcgcg gcgttcagcg cgatggttgc
     1021 cagaatgaaa ccgaggggct ccagaagcat gacgtacccg acgacgagcc ccaatccgac
     1081 aacggcaagc agtatctgcc gcgggaagag atcggcgagc gtccgcccgt cgtcgtccat
     1141 tttgccgcga aaattcaacc atcccaagac gagagcgagc ccagcagtca tccaggcgag
     1201 cagttccggg aaaaaagatg cgccgacgta atcgtcctgg atgagtgtct gttcgaagtt
     1261 attcgcctgg tagatcacga atgcgctcag caaaagaaaa acaagtacga tgccgtagtt
     1321 ggcttttttc acgtgacatt ctcctccgaa attatctgtg gagcgttggg aaagggcggg
     1381 aggccgccgt cggaaccggc gccccccgcg tggttacgtc ctgtaacgaa aacgaagcgt
     1441 tctcttattg ctgctgctgt tttttcactt cttcaacgac tgcgtcgagg gccttcacgt
     1501 cgttctccac gaaggttttg aagtcggctg cgttcaggaa acggatcccg agcgtgcggc
     1561 cgttcatgaa gtcgacgaac tccttctcgg cgacagcttt ctccaaggcc gctccaagaa
     1621 ccgccttcac gtcctcgggc gttcccttgg gaaccgcgat gcctctccac gtaccggtga
     1681 cgataggata cccgagctcg gtcatcgtcg gcacgtcggg aagcccggcc atgcgaacgt
     1741 cggagcaga
//
LOCUS       DAOSWW010000632         1749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_58430_length_1749_cov_3.340024, whole genome shotgun sequence.
ACCESSION   DAOSWW010000632 DAOSWW010000000
VERSION     DAOSWW010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58430_length_1749_cov_3.340024"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PK442_11345"
     CDS             complement(<1..804)
                     /locus_tag="PK442_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HOO88290.1"
                     /translation="MYAETSFVLAVMVFTFALASWKLKSPELSMVITAVVGALAGGLG
                     FPVRLLVEGTFTYFDVAFTFLTASIFINFYSATGAMDALVRKMVDRFFDRKWILFFLL
                     AVIMLIPGALTGAGSVSMFVVGGMVATVLRFSGIKETRIVAFIYVTSMLAAVAPPINL
                     WVMLMCAQANMPYVGFEFPLLAPIALITVFTVFYLLRGGSPKDKETVLASLPEAHERM
                     NWFRIAAPLLVFVVLVLLSKYAAFSTPVLGIPLIFIISTAVAVMLSPERK"
     gene            complement(868..1452)
                     /locus_tag="PK442_11350"
     CDS             complement(868..1452)
                     /locus_tag="PK442_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOO88291.1"
                     /translation="MRKIAAYSLLVCLLFASVSFAAVTGAEAPKGEEPLLITNAGQGP
                     GGKMGRLLVARAEVVEQLTYNAEPKPEDIAAGGYKTILVVIGSSAKGLGASGITIDQE
                     VDRLHAIMEKAKELNIPVIAAHIEGKARRGKPGSADERSIDAILPYAAHIVVNKDGDL
                     DKKFTRFGEEHSIPVSYLDNAMDLNAFAKTMYAK"
BASE COUNT          396 a          477 c          537 g          339 t
ORIGIN      
        1 cttccgctcc ggggagagca tcacggcgac ggccgtgctg atgatgaaga tgagcggaat
       61 gccgagcacc ggcgtcgaga aggccgcgta cttggagagg aggacaagca cgacgaagac
      121 cagcagcggc gcggcgatac ggaaccagtt catccgttca tgcgcttcgg gaagggaggc
      181 gagcaccgtc tccttgtcct tgggggagcc tcctcgcagc aggtagaaga ctgtgaagac
      241 cgtgatgagc gcgatggggg ccagaagagg gaactcgaac cccacgtagg gcatgttggc
      301 ctgcgcgcac atgagcatga cccagaggtt gatcggcggc gcgacggcgg ccagcatcga
      361 cgtgacgtag atgaaggcga cgatgcgcgt ctccttgatg ccgctgaagc ggagcaccgt
      421 ggcgaccatt ccgccgacaa cgaacatgga gacgctcccc gcgccggtca gcgcgccggg
      481 aatgagcatg atgacggcga gcagaaagaa aagaatccac ttgcggtcga agaatcggtc
      541 caccatcttg cgcaccagcg cgtccatcgc tccggtggcc gagtagaagt tgatgaagat
      601 ggaggccgtc agaaaggtga aggccacgtc gaagtaggtg aacgtccctt cgaccaggag
      661 gcggaccggg aatcccagcc ctccggcgag cgcgccgacg acggcggtga tcaccatcga
      721 cagttcgggt gatttcagct tccagctcgc gagggcgaag gtgaacacca tcacggccag
      781 aacaaacgat gtttccgcgt acatctcagg acctcccgtc cgttgaaaag aaaagacccc
      841 gtcgaaagat acggcggggt ccggttttta ttttgcgtac attgtttttg cgaatgcgtt
      901 gaggtccatc gcgttgtcga ggtagcttac gggaattgag tgctcttcac cgaagcgggt
      961 gaactttttg tccagatcgc cgtccttgtt caccacgata tgcgcggcgt acggcagaat
     1021 ggcgtcgatg gagcgttcgt cggcgctccc cggtttaccg cggcgggcct tgccctcgat
     1081 atgcgcggcg atgaccggga tattcagctc cttggccttc tccatgatcg cgtgcagacg
     1141 gtcgacctcc tgatcgatcg tgatgcccga agcgccgagc ccctttgcgg aggagccgat
     1201 gacgacgagg atggtcttgt atccgccggc cgcgatgtcc tcgggcttcg gttcggcgtt
     1261 gtaggtgagc tgctccacga cttccgcgcg ggcgaccagc agacggccca tcttgcctcc
     1321 ggggccctga cccgcgttgg tgatcagcag gggttcttct cccttgggcg cctcggctcc
     1381 ggtaacagcc gcgaacgaga cggacgcgaa cagcaggcag acgagcagag agtacgctgc
     1441 gatttttctc ataacaaaga aacctccttt ggaattatga taaagaaaca taccgaatac
     1501 atatcggtaa acgatacgtg gtgttacttc ttgaaataaa aatgtacttc cttgcatttt
     1561 cggatgcgat gcacggcgta gaatgttgta tatatcaata acgtcaatga tatacctccc
     1621 tttgaacgat gtcaacatag accggacaat ttcggaagaa tcagcggttg cgtgaataca
     1681 atatacatga gcagcgagtc gctcaaatga aacgccggac tcttcaaaaa agagtccggc
     1741 gttcactca
//
LOCUS       DAOSWW010000633         1748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_58527_length_1748_cov_1.868281, whole genome shotgun sequence.
ACCESSION   DAOSWW010000633 DAOSWW010000000
VERSION     DAOSWW010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58527_length_1748_cov_1.868281"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PK442_11355"
     CDS             <1..779
                     /locus_tag="PK442_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO88292.1"
                     /translation="SRYERLQSLFQAGGTSKQELDKANVAVQEESAKLRDARTTVSRT
                     QIRSALNGVVVRRSVERGEFAEPGRELLAIADFDRREVEMLISPTIRGRIRSGMDVLV
                     KAPWGGQTMGKIFRMDPEADPATGFFRGIVRLPSECTLIPGDYVRMEVRMVHKENTLA
                     VPYESILREDGKPHVFVVSGDKAARRDIVVGEGSGGFVEISSGLVPGDKVVKIGAGSL
                     YDGAKILITNGALRTGGGSGGGQPKPAEPRTASPDVAGEK"
     gene            783..>1748
                     /locus_tag="PK442_11360"
     CDS             783..>1748
                     /locus_tag="PK442_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO88293.1"
                     /translation="MGIIGISIRRPVLTLVIVILAVLLGLSGMQGMGISLLPRIEVPF
                     VSVMTSYTGAGPEDMETLVSKPLEDAISQVQGVRRIESTSLEGLSYVFIEFDSEINLA
                     DAALDVANRVRTVNLPEDADDPIVRKFDINARSFMSVVFTSTLPPQRARDILEDRVQR
                     QLSQVVDVADVSLDGGLTREIHVDLDPVALGSLGLSIRQVTNLIKSGNYSSPSGRISL
                     GDQESILRVVGESVSVRELEELQIPLAKGVSVRLADIATVTDATAEVRRLARYNGRDS
                     FIISLTAVPDGNVVRASREARTMLDQIVLTLPPGFSMAIPFDDGEF"
BASE COUNT          339 a          487 c          537 g          385 t
ORIGIN      
        1 aatcccgcta tgaacgactg cagagcctct ttcaggccgg tggaacgtcg aaacaggaac
       61 tggacaaagc gaacgttgcc gttcaagaag agtcggcaaa gcttcgtgac gcgcgtacga
      121 ccgtttcccg tacgcagata cgttcggcgc tcaatggcgt tgtcgttcgg cggagtgtgg
      181 agcggggaga gttcgcggaa cccgggcgcg agttgctggc gatcgccgat ttcgaccgac
      241 gcgaagtgga gatgcttatc tctcctacaa tacgcggccg catccgttcc ggtatggatg
      301 tgcttgtgaa agccccctgg ggcggtcaaa caatgggaaa aatcttccgc atggatcccg
      361 aggcggaccc ggcgaccgga ttcttccgcg ggattgtccg tctcccctcg gagtgcaccc
      421 tcatccccgg cgattatgtc cggatggagg tgcggatggt ccacaaggag aacacgcttg
      481 ccgtaccgta tgaatccatt cttcgcgagg acgggaaacc tcatgtcttc gttgtctccg
      541 gcgacaaggc cgccaggcgc gatattgtcg taggggaggg gagcggaggt tttgtcgaga
      601 tctcctccgg tcttgtaccc ggcgataaag ttgtgaagat tggagcgggc agcttgtacg
      661 atggagcgaa gatcctcata accaacggcg ctttacgcac cggcggcggt tccggaggcg
      721 gacagccgaa accggcggaa ccccggacgg caagtccgga tgtcgccgga gagaaataaa
      781 ccatgggtat catcgggata tcgatcaggc gccccgtcct gactctggtc atcgtcatcc
      841 tggctgtcct cttgggactc tcgggtatgc aggggatggg catatcgctt cttccccgga
      901 tagaggttcc ttttgtctcc gtcatgacga gctacaccgg agcaggtccc gaagatatgg
      961 agacgctcgt ttccaagccg cttgaggacg ccatcagcca ggtgcaggga gtcaggagga
     1021 tagagagcac ttctctcgaa ggcctttcgt atgtcttcat agagttcgac tcggagatca
     1081 atcttgcgga cgccgcgctc gacgtggcaa accgggttcg gacggtgaat cttccggagg
     1141 atgcggacga tcctatcgta cggaagttcg atatcaacgc gcgttctttc atgtccgtcg
     1201 tgtttacctc gacgcttccg ccccagaggg cgcgtgatat ccttgaggac cgcgtgcagc
     1261 gccagctctc tcaagtggtc gatgtcgccg acgtttctct ggacggcggt ctgacaaggg
     1321 agattcatgt cgacctcgac ccggttgcgc tgggcagtct ggggctgagc atccgtcagg
     1381 tcaccaacct gatcaaaagc gggaattaca gctctccttc ggggcgtatc tctctgggag
     1441 atcaggagtc aattcttcgc gtagttggag agtctgtttc ggttcgtgaa cttgaagagc
     1501 tccagattcc tcttgcaaag ggcgtttccg tacgtctcgc cgatattgcg actgtcaccg
     1561 atgcaactgc ggaagtgcgg cgtcttgccc gttacaacgg gcgcgattcg ttcataatct
     1621 cgctcacagc cgttccggac ggcaatgtcg tccgtgcgag ccgcgaggcc aggaccatgc
     1681 tcgaccagat tgtgctgacg cttccgcccg gcttttctat ggccattccc ttcgacgacg
     1741 gcgagttc
//
LOCUS       DAOSWW010000634         1746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_58600_length_1746_cov_3.071555, whole genome shotgun sequence.
ACCESSION   DAOSWW010000634 DAOSWW010000000
VERSION     DAOSWW010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58600_length_1746_cov_3.071555"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PK442_11365"
     CDS             <1..418
                     /locus_tag="PK442_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOO88294.1"
                     /translation="VIGKRCRSVSPEDALGYVLGYTCANDVTARDLQSKDGQWTRSKS
                     FDTFCPLGPFVVSGIDPSDLEITMLVNGKVVQHSRTSNFIFPVPVLVSHISHVMTLEP
                     GDVIITGTPAGIGPVKKGDLMRVEIERLGALENRVE"
     gene            complement(515..600)
                     /locus_tag="PK442_11370"
     tRNA            complement(515..600)
                     /locus_tag="PK442_11370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(564..566),aa:Ser,seq:tga)
     gene            604..1170
                     /locus_tag="PK442_11375"
     CDS             604..1170
                     /locus_tag="PK442_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOO88295.1"
                     /translation="MKSIIHIFCTLANVLREERGPVFRFFRVGYNSVVLKKSACGNDS
                     SGEVIGVNKEEILKQYVCSPCRVAIVGASPKADRPVFRVMNYLSGAGFTLFPVNPGYA
                     GVEILGLPCVADLASLKEGVDVVALFLSEKMQGAVEEDLKQLSYKPVVWFQPGAQNEP
                     LANSLAGAGYPVVEEDCLMAAHMNKCGV"
     gene            1303..1728
                     /locus_tag="PK442_11380"
     CDS             1303..1728
                     /locus_tag="PK442_11380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOO88296.1"
                     /translation="MSSEIPFTLRVRYGDTDQMGVAYYANYLYWFEVGRTEFCRALGR
                     SYADWEAEGVFLPVVESHCRYKHPARYEDEITIYTKIGEIRASSIVFECRIERASDSK
                     PLAAGWTRHAFVDADGRLIRGDNPMRRWIEALRDDGGVA"
BASE COUNT          358 a          451 c          546 g          391 t
ORIGIN      
        1 cgtcatcggg aagaggtgca ggagcgtgtc gccggaggat gcgctcggct acgttttggg
       61 gtacacctgc gcgaacgacg tgaccgcgag ggatcttcag tcgaaggacg ggcagtggac
      121 gcggtcgaag tccttcgata cgttctgtcc gctcgggccg tttgtcgtgt ccgggatcga
      181 tccgtcggac ctggaaatta caatgctggt gaacggcaag gtcgtccagc actcccgcac
      241 gagcaacttc atcttccccg tgccggtgct cgtcagccac atctcgcacg tgatgacgct
      301 ggaacccggc gatgtgatca tcacaggcac cccggctgga atcgggccgg tgaagaaggg
      361 ggatctgatg cgcgtcgaga tcgaacgtct gggcgcgctg gagaaccggg tcgagtagcc
      421 tgattcggaa gcatacgcaa aaagggggac cgcgttccgc tcgcgttccc ccttttcgtc
      481 tgcggacgta aaaacgtccg atcatgcatg tgaatggcgg agggcagagg attcgaaccc
      541 ctggggctta cgcccagttg atttcaagtc aaccaccatc gaccgctcgg acagccctcc
      601 agcatgaaga gcattataca catcttttgt actcttgcca acgtgctgcg tgaggaaaga
      661 gggcctgttt ttcgcttctt ccgcgtgggg tataatagcg ttgtactcaa gaagagtgca
      721 tgcggcaacg attcttcggg agaggtgatt ggagtgaata aagaagaaat tttgaaacaa
      781 tatgtctgct caccctgcag agtggcgatt gtcggagcgt cgccaaaggc cgaccgtccg
      841 gtatttcgtg tgatgaacta cctttccggc gccggtttta cgctctttcc ggtgaatccg
      901 gggtatgcgg gcgtcgagat cctggggctt ccgtgcgtcg ccgaccttgc ttccctgaaa
      961 gagggtgtcg atgttgtcgc gctctttctc tccgagaaga tgcagggtgc ggttgaggag
     1021 gatctgaaac agctctccta caagcctgtg gtgtggttcc agccgggagc gcagaatgag
     1081 ccgcttgcaa actcgctcgc cggggcgggg tatcctgtgg tggaagaaga ctgcctgatg
     1141 gcggcgcata tgaataagtg cggcgtatga ttttgttgtt gcgtaaaaaa tagtatttgg
     1201 tatataattc accacaagga gtgagttctt gttgaaaacg cgcgcatcac atgcgttttt
     1261 caagatgttt gtttcactcc accttttccg ggaggtgttc ccgtgtcctc cgaaattcct
     1321 ttcacactgc gcgttcgtta cggcgatacc gaccagatgg gcgtcgcgta ctacgccaac
     1381 tatctctact ggttcgaggt cgggcggacg gagttctgcc gggctcttgg caggtcgtac
     1441 gccgactggg aggccgaggg agtattcctc cccgtcgtcg agtcgcactg ccggtacaag
     1501 catcctgctc ggtacgagga tgagatcacc atatacacga aaattggtga aatacgggcg
     1561 agcagcatcg tcttcgaatg tcgcatcgaa agggcgtccg acagcaagcc tcttgccgcc
     1621 gggtggaccc gccatgcgtt tgtcgacgcg gacggcaggc tgatacgggg agacaacccc
     1681 atgcgccgct ggatagaggc gctgagggat gatggaggtg tcgcgtagag tgagcacaaa
     1741 agccat
//
LOCUS       DAOSWW010000635         1741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_58882_length_1741_cov_4.298932, whole genome shotgun sequence.
ACCESSION   DAOSWW010000635 DAOSWW010000000
VERSION     DAOSWW010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58882_length_1741_cov_4.298932"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="PK442_11385"
     CDS             complement(<1..145)
                     /locus_tag="PK442_11385"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HOO88297.1"
                     /translation="MGTTLRVKEIVGEQVALRGRTDLEFDVAFCPEFLKEGTAVEDFM
                     KPDR"
     gene            568..792
                     /locus_tag="PK442_11390"
     CDS             568..792
                     /locus_tag="PK442_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOO88298.1"
                     /translation="MPAVRIPEDLDNRLNTLARRTGRSKNSFVREALLHQLDDIEDYY
                     LGMETLERIRNGEEKLYSSAEARRELGLDD"
     gene            776..1039
                     /locus_tag="PK442_11395"
     CDS             776..1039
                     /locus_tag="PK442_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO88299.1"
                     /translation="MAWTIEYSEGALRTLKKMSPATARRIFGHMDKIASSETIRSTGK
                     ALKGNLGTLWRYRVGDYRIVCTIDDKILRVLVVKVGHRSEVYR"
     gene            1092..1328
                     /locus_tag="PK442_11400"
     CDS             1092..1328
                     /locus_tag="PK442_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88300.1"
                     /translation="MEKEVAPMSYSKTMKTFAVMSQDDFRQMTLDIASGAYRPSPDAP
                     RKVFCSFSGLAEYARKEAEKEKMRDLPLCKTTSQ"
BASE COUNT          417 a          540 c          421 g          363 t
ORIGIN      
        1 tccggtccgg cttcatgaag tcctccaccg ccgtcccctc tttcaggaac tccgggcaga
       61 aggccacgtc gaactccagg tccgttcgcc cgcgcagcgc cacctgctcg cccacgatct
      121 ccttcacccg cagcgtcgtc cccaacggca agagtcacgt ttcgacaagc ttcgcaaggc
      181 gagcgccacg cgccccaagt acgccccgaa aagcagcttt ctcgctccgc gttctcaggt
      241 aacacctcaa catcatccga tgcacacgca ctcgaacccc acgacttcat cgtgggggga
      301 gaatacgggt gcctcccgtc gaagatcagc ggagtcttca acagatcctt catcctcggg
      361 aagtccagcc tccggaagag cgcccactcg ctcagcagca gcagcgcatc cgcgccctcc
      421 agcgcttcgc agttctcctc cattttccga caatatgctt tcaatcccct gtttctctgt
      481 gctatcatag tacttacagc gaaatgggtt ctatccatac catatccgga aaccatacat
      541 gaaagcgctg gaaagaggtg acactgtatg ccggctgtac gaattcccga agatctcgac
      601 aatcgcttga acaccctcgc tcgccgcacc ggacgttcca agaactcttt cgtgcgcgaa
      661 gcgttgctgc accagctcga cgacatcgag gactactatc tcggcatgga gaccctcgaa
      721 cggatacgca acggagaaga aaagctgtac tcatcggcgg aagcgaggcg cgagcttggc
      781 ctggacgatt gaatactcgg aaggggcgct ccgaacgctc aagaaaatgt ctcctgcaac
      841 agcccgtcga attttcggtc acatggacaa aatcgcctcg tccgaaacta ttcgctccac
      901 aggcaaggcc ttgaaaggaa acttgggaac tctctggcgc tatcgcgtcg gcgactaccg
      961 aatcgtatgc accatcgacg ataaaatcct tcgagtgctt gtcgtcaaag ttggacatcg
     1021 cagcgaggtc tatagataaa ttccaagcga cttttcggag atatcggatc ttgtggaggt
     1081 ctacagatat attggaaaag gaggttgctc caatgagcta ttctaagacc atgaaaacct
     1141 ttgccgttat gtcgcaggat gattttcggc agatgacatt agatattgca tcgggcgcgt
     1201 accgtccttc tcccgacgct ccgcgcaaag tattctgctc attctccggt ttggcggaat
     1261 acgcccgaaa agaagccgaa aaggaaaaaa tgcgcgatct tccgctatgc aagacaacgt
     1321 cgcagtaaat cgaacaccgt atcattgagt tgatccgaaa tcctcgcatt ttcaaaacat
     1381 agacaactcg cctttctctc gaacctcacc cccgcccgat gcacacgtac tcaaatccca
     1441 tcgattgcat cgcggcgggc gaatactggt ttctcccgtc gaagatcaga ggcgtcttca
     1501 gcagctcctt catcctcggg atgagcagcg aattatgcta gagcgcccgt gctggaatag
     1561 catcgtcaca cagaaaaaaa ccacatccca atgtcgtgcc aagtccgttc ccgctcacgc
     1621 ggagcgcggc ttggcgggag ttgtcctcag ctccacccca agggccagca acagcttcag
     1681 gacagtgtcg taacggggtt ttgcgccagg ggccagcgcc ttgtacagac tctcgcgccc
     1741 c
//
LOCUS       DAOSWW010000636         1741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_58907_length_1741_cov_2.905101, whole genome shotgun sequence.
ACCESSION   DAOSWW010000636 DAOSWW010000000
VERSION     DAOSWW010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58907_length_1741_cov_2.905101"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK442_11405"
     CDS             complement(<1..656)
                     /locus_tag="PK442_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOO88301.1"
                     /translation="MRAVVKYKREDGNTEVRDVPVPEIEASDVLVKVAYIGICGSDPH
                     MYHNQVSYPMAIPLILGHEFSGTIEKVGTDVKKWKVGDRVTAETHARFCGKCVLCKTN
                     NYRFCRERKGYGFGVDGAFAEYVRVPERILHAVPDSVTLRDASCTEPVCVAYNVVIAK
                     TGVKAGDCVAVLGPGPIGLLCAHMAKLAGASHIAVFGAPGDEQRLVIAKQYGATETHL
                     L"
     gene            complement(706..>1741)
                     /locus_tag="PK442_11410"
     CDS             complement(706..>1741)
                     /locus_tag="PK442_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO88302.1"
                     /translation="GKDPLKGWIVGFLGFAAAMVGMEQIFAYPRLTFNFLQLKGGIPL
                     IPALIGVFGISEFLAVLQENVAYKIDGKVGKIIPPMKEFIPLIPSAIRSGVIGVVIGA
                     IPGAGEDIGSWLSYDVGKRRSKQGHKFGTGSYEGLVCSEVANNAVIGGAMIPLLTLAV
                     PGSPPSAMFLAAVWLHGVRPGPMLNFENPGFLPLVAAMLILGSFAMLFWGLALAKPMV
                     AILKIKREILLPIVIPLTVIGAYAGGVRIFDVYLMLAFGVLGYILRQMDYPLAPLVLG
                     LILGPLADTSFRRALQQSRGAIMPLLERPIGLLLLLCIAWLIWTGIKRTRQYYAEQTA
                     REDGEELSAE"
BASE COUNT          320 a          539 c          565 g          317 t
ORIGIN      
        1 aggaggtgcg tctcggtcgc gccgtactgt ttcgcaatga caaggcgctg ttcgtcgccg
       61 ggcgcgccga agacggcgat gtggctcgca ccggcgagtt tcgccatgtg ggcgcagagc
      121 agccctatcg gtccgggacc gaggacggcg acgcagtcgc ccgccttgac gcccgtcttc
      181 gcgatcacca cgttgtacgc cacgcagacg ggctccgtgc aggaggcgtc ccgcagcgta
      241 accgaatcgg gaacggcgtg gagaatacgc tccggcacgc ggacatactc cgcgaacgcg
      301 ccgtcgacgc cgaaaccgta ccccttgcgc tcgcggcaga agcggtagtt gttcgtcttg
      361 cagaggacgc acttgccgca gaagcgggcg tgcgtctccg cggtcacccg gtcgccgacc
      421 ttccattttt tcacgtcggt cccgaccttt tcgatggtcc ccgagaactc gtgtccgagg
      481 atgaggggga tcgccatggg gtaggagacc tggttgtggt acatgtgcgg gtcgcttccg
      541 cagatgccga tatacgcgac ctttacgagt acgtccgacg cttcgatctc cggtaccggg
      601 acgtcccgta cttccgtgtt gccgtcttct ctcttgtatt tcaccactgc gcgcatagtc
      661 cgttctccct cctcgccgcc gggaacatcg gcggcgtttc gttgtctatt ccgcggagag
      721 ctcctctccg tcctctcttg cggtctgttc ggcatagtac tgtcgtgttc gtttgatccc
      781 cgtccagata agccacgcga tgcacaacaa aagcagcagc ccgatggggc gctcgagcag
      841 cggcatgatt gcgccgcggc tctgctggag ggcccggcgg aagctggtgt cggccagcgg
      901 accgaggatg agtccaagga cgagcggagc cagcggatag tccatctgcc gcaggatgta
      961 ccccagaacg ccgaaggcga gcatcaggta gacgtcgaaa atacggacgc ctcccgcgta
     1021 cgccccgatg acggtcaggg ggatgacgat ggggagcagg atctcccgct tgatcttcaa
     1081 aatggccacc atcggtttgg cgagcgcgag cccccagaag agcatggcga acgagccgag
     1141 gataagcatg gccgccacga gggggagaaa gccggggttt tcgaagttga gcatcgggcc
     1201 ggggcggacg ccgtgaagcc agacggccgc aaggaacatt gccgaggggg ggcttcccgg
     1261 tacggcgagg gtgagcagcg ggatcatcgc gccgccgatg acggcgttgt tcgccacttc
     1321 ggaacagacc agcccttcgt agctccccgt gccgaacttg tgcccctgtt tggagcgcct
     1381 cttgcccacg tcgtaggaga gccagctccc gatatcctcg ccggctcccg gaatggcgcc
     1441 gatgacgacg ccgatgacgc ccgagcgaat tgcggacggg atgagcggaa tgaactcctt
     1501 catcggcgga atgatcttcc cgacctttcc gtcgatcttg tacgctacgt tctcctgaag
     1561 cacggcgaga aactccgaaa tgccgaagac gccgatgagc gccggaatga gcgggattcc
     1621 tcctttgagc tgaaggaaat tgaaggtcag gcgggggtag gcgaaaatct gctccatccc
     1681 caccatggcg gcggcgaagc cgaggaaacc gacgatccac cctttgagcg ggtccttccc
     1741 g
//
LOCUS       DAOSWW010000637         1738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59072_length_1738_cov_2.870469, whole genome shotgun sequence.
ACCESSION   DAOSWW010000637 DAOSWW010000000
VERSION     DAOSWW010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59072_length_1738_cov_2.870469"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PK442_11415"
     CDS             <1..420
                     /locus_tag="PK442_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HOO88303.1"
                     /translation="DSLRFEAGLPLCGHEYTETIGPLEAGYGFFVKVDKAGGFIGQPV
                     LKRQKEEGLKHRTVALRLEDKGVPRHGMDVANAEGRVVGHVTTGGYSPSLDANIASCY
                     VETPAPAEGEHLWIVIRDKPKQGVVVKRPFYAKSYKS"
     gene            456..854
                     /gene="gcvH"
                     /locus_tag="PK442_11420"
     CDS             456..854
                     /gene="gcvH"
                     /locus_tag="PK442_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOO88304.1"
                     /translation="MAKIPQDLHYTKDHEWIKIVDGMGVMGISDYAQHAMGDVVYVEL
                     PDVGRKAKAGEDFIVVESVKGANDVFCPVSGTVAGVNEDLDGAPELLNQDPYSNWLVK
                     MEVDNPSDVDKLLDAAAYAKLVEELEAEES"
     gene            867..>1738
                     /gene="gcvPA"
                     /locus_tag="PK442_11425"
     CDS             867..>1738
                     /gene="gcvPA"
                     /locus_tag="PK442_11425"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOO88305.1"
                     /translation="MNRYIPVTAEDRRRMLGVIGAASVDDLFSGIPVQVRQKKASDLP
                     KALSEPEMLRDLRALSGKNLNADDAICFLGAGVYDHFIPSAVDSIVSRAEFTTSYTPY
                     QPEVAQGTLQAIFQYQTMVCELTGMEISNASMYDGASAAAEAAVLAASATKKRTVLCG
                     RNVHPQTRQTVRTYCWSRRLEYAELPAENGRIDRAALAKALEGGDAAALIVQSPNFFG
                     NLENLQELGDAVHEAKGLFIVSSDLLALGTLEAPGKLGADIVVGDGQTAGNPMNFGGP
                     AFGFIAVNKPLMRK"
BASE COUNT          357 a          562 c          552 g          267 t
ORIGIN      
        1 gacagcctcc gcttcgaggc gggactcccg ctgtgcggcc acgagtacac cgagactatc
       61 ggcccgctgg aggcggggta cggcttcttc gtgaaggtgg acaaggcggg cggcttcatc
      121 ggtcagcccg tactcaaacg ccagaaggaa gagggattga aacaccgcac cgtggcgctg
      181 cgcctggagg acaagggcgt tccccggcac ggcatggacg ttgcgaacgc cgaggggcgc
      241 gtcgtcgggc acgtgacgac gggcgggtac agcccctcgc tcgacgccaa catcgcctcg
      301 tgctacgtgg agacccccgc gcccgccgag ggagagcatc tctggatcgt catccgcgac
      361 aagccgaaac agggcgtcgt ggtcaagcgc ccgttctacg caaagagcta caagagctga
      421 aaactgaaac caaaaacaag aggaggatga acagcatggc aaaaataccc caggatcttc
      481 actacacaaa ggatcacgag tggatcaaaa tcgtcgacgg catgggcgtc atgggcatca
      541 gcgactacgc gcagcacgcg atgggcgacg tggtctacgt ggagcttccc gacgtcggca
      601 gaaaggcgaa ggccggcgag gacttcatcg tcgtggagtc ggtgaagggc gcgaacgacg
      661 tcttttgccc cgtctccggc acggtcgccg gggtcaacga ggatctcgac ggcgcgcccg
      721 agctgctgaa ccaggatccg tactccaact ggctggtcaa gatggaggtg gacaatccct
      781 ccgacgtgga caaactcctc gacgccgccg cgtacgccaa gctcgtcgag gagctggaag
      841 cggaagagtc gtaagggggg atcgccatga accgttatat tcccgtcacg gcggaggatc
      901 gtcgccggat gctcggcgtc atcggcgccg cgagcgttga cgacctcttc tccggaatcc
      961 ccgtgcaggt ccgtcagaag aaggcctccg atctgccgaa ggccctttcc gagcctgaga
     1021 tgctccgcga tcttcgcgct ctctcaggaa aaaatctgaa cgcggacgac gcaatctgct
     1081 tcctcggcgc gggggtctac gaccacttca tcccctcggc ggtcgacagc atcgtctcgc
     1141 gcgcggagtt caccaccagc tacaccccct accagccgga ggtggcgcag ggaacgctcc
     1201 aggccatctt ccagtaccag accatggtct gcgaactcac cggcatggag atctccaacg
     1261 cctccatgta cgacggcgcc tccgccgccg ccgaagccgc tgtgcttgcg gcctccgcga
     1321 caaaaaagcg tacggtactc tgcggccgga atgtgcatcc gcagacgagg cagactgtcc
     1381 gtacctactg ctggagccgg cggctcgagt acgccgaact ccccgcggag aacggccgga
     1441 tcgaccgtgc ggcgctggcg aaggcgctcg aaggcgggga cgccgccgcg ctgatcgtcc
     1501 agtcgcccaa cttcttcggc aacctggaga acctgcagga actcggagac gccgttcacg
     1561 aggcgaaggg gctcttcatc gtctcctccg acctgctcgc gctgggaacc cttgaagcgc
     1621 ccggaaaact cggagctgac attgtcgtcg gcgacggcca gacggcgggc aacccgatga
     1681 acttcggcgg ccccgccttc ggcttcatcg ccgtgaacaa gcccctgatg cggaagaa
//
LOCUS       DAOSWW010000638         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59182_length_1736_cov_3.081499, whole genome shotgun sequence.
ACCESSION   DAOSWW010000638 DAOSWW010000000
VERSION     DAOSWW010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59182_length_1736_cov_3.081499"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK442_11430"
     CDS             complement(<1..341)
                     /locus_tag="PK442_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HOO88306.1"
                     /translation="MKERILKRADELKSELLRLSHDIHANPETSFEEFRAAAWQKELL
                     EKHGFTVEAPFCGMETAFRAVKKSGKPGPVVAFLSEYDALNGLGHACGHNIIAASSVG
                     AGLALASVLDET"
     gene            complement(405..1403)
                     /locus_tag="PK442_11435"
     CDS             complement(405..1403)
                     /locus_tag="PK442_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88307.1"
                     /translation="MTKLLEVTNLEQRFDLNTDFLSKLVFEKGRIVKRERIVKAVNGV
                     SFSVGRREVFSIVGESGCGKSTVARTVVKLIEPKGGRICYDGRDITELSRTEMLPFRK
                     RMQMIFQDPYASLNPRQRVADILVEPMLFHKIARSPEDAREKALEILNRVGIRTEQAS
                     RYPHQFSGGQRQRIGIARALAVEPEFIVADEPVSALDVSIQAQILNLLMDLKEEFSFS
                     YLFIAHDLSVVKHISDNLAVMYLGRMVEKGSKAHIFGNPLHPYTLALFSAIPRISGTK
                     MRTGRNLEGEIPSAIDLPPGCCFHTRCPKALPVCREVVPQEREIEPGHFVSCHLFA"
     gene            complement(1400..>1736)
                     /locus_tag="PK442_11440"
     CDS             complement(1400..>1736)
                     /locus_tag="PK442_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO88308.1"
                     /translation="MADRINVMYCGKIVETGPARKVIDESAHPYTKGLIDSIPDLERD
                     RKRLNTIKGIVPNMFELPEGCNFAPRCAHATELCFGVEPEQREIGAGHFVACHFPLGG
                     PAHEEEARS"
BASE COUNT          299 a          559 c          534 g          344 t
ORIGIN      
        1 gtttcgtcga gaacggaagc gagcgcgagc ccagcgccga cgctcgacgc cgcaatgatg
       61 ttgtgcccgc aggcgtgccc cagcccgttg agcgcgtcgt actcggagag gaaagcgacg
      121 accggaccgg gcttcccgct ctttttcacc gcgcggaagg cggtctccat gccgcagaaa
      181 ggcgcctcca ctgtaaatcc gtgcttttcg agcagctcct tctgccatgc ggcggcccga
      241 aactcctcga aggaggtctc ggggttcgcg tggatgtcgt ggctcaggcg cagcagctcc
      301 gacttcagtt cgtccgctcg tttcagaata cgttctttca tttgtacacg tcctttatcg
      361 ttggtatggc atcgaggccg ggggaagaac cccgcctctc ccgtctatgc aaaaagatgg
      421 cacgaaacga agtgccccgg ttcgatctcc cgctcctgag gcacaacctc ccggcagacc
      481 gggagcgcct tcgggcagcg cgtatggaaa cagcaccccg gcggaaggtc gatcgcgctg
      541 gggatctccc cctccaggtt gcgcccggtg cgcatcttcg ttcccgaaat gcggggaatc
      601 gccgaaaaga gggcgagcgt gtaggggtgc agcgggtttc cgaagatatg cgccttgctc
      661 cccttttcca ccatcctccc gaggtacatc accgcgaggt tgtcgctgat gtgcttcacc
      721 acggagaggt cgtgcgcgat gaagaggtac gagaaggaaa actcttcctt caggtccatc
      781 agcagattga gaatctgcgc ctgaatcgag acgtcgagcg cggagaccgg ctcgtcggcg
      841 acgatgaact ccggttccac ggcgagcgcg cgcgcgatgc cgatccgctg ccgctgccct
      901 ccgctgaact ggtgcgggta gcgcgacgcc tgctcggtgc ggatgccgac cctgttcaga
      961 atttcgagcg ctttctcccg cgcatcctcc ggcgaccgcg caatcttgtg gaagagcatc
     1021 ggctcgacga ggatgtccgc cacccgctgc cttggattca gcgaggcgta ggggtcctgg
     1081 aagatcatct gcatccgttt ccggaagggg agcatctccg tgcgggagag ttcggtaata
     1141 tctctgccgt cgtagcaaat ccgccccccc ttcggctcga tgagcttcac cacggtgcgg
     1201 gcgacggtcg acttgccgca cccggattcg cccacgatgc tgaagacctc gcgcctgccg
     1261 acggagaagc tgacgccgtt gacggccttc acgatccgct cgcgcttgac gatacgtccc
     1321 ttctcgaaga cgagcttgct caagaagtcg gtattcaggt cgaagcgctg ttccaggttc
     1381 gtcacttcaa gcagtttcgt catgagcgcg cctcctcttc atgcgccggc ccgcccagcg
     1441 ggaagtggca ggcgacgaaa tgccccgccc cgatttctct ctgctccggc tcgacgccga
     1501 aacacagctc cgtagcgtgc gcgcagcggg gggcgaagtt gcacccctcg ggcagctcga
     1561 acatgttggg gacgatgccc ttgatggtgt tcagccgctt cctgtcgcgt tccaggtcgg
     1621 ggatggagtc gatcaacccc ttcgtgtagg ggtgcgcgct ctcgtcgatg accttccgcg
     1681 ccggaccggt ctccacgatc ttgccgcagt acatcacgtt gatccggtcg gccatc
//
LOCUS       DAOSWW010000639         1735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59221_length_1735_cov_3.767857, whole genome shotgun sequence.
ACCESSION   DAOSWW010000639 DAOSWW010000000
VERSION     DAOSWW010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59221_length_1735_cov_3.767857"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PK442_11445"
     CDS             <1..675
                     /locus_tag="PK442_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88309.1"
                     /translation="NALGYWEGNRIYREYHVYIDWRWLSMELATLAVAVGLLWRWKLP
                     FMLMPVALTLWYMSMDLAPFLFKNEDLTWELRRTVSLWFGLAVLCFAIFVDFRTRSRK
                     DYAFWLYLCGLAAFWGGLSLMDSDSELGKFVYFCINIFLIAVGALLSRRAFAVFGGFG
                     AAGYIGHLTWRVFSDSVVFPFVLSFAGFFVIWLGIRWQKKEKQITALLRSFLSPEMRS
                     FLERNE"
     gene            784..>1735
                     /locus_tag="PK442_11450"
     CDS             784..>1735
                     /locus_tag="PK442_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HOO88310.1"
                     /translation="MEFIVFLVIAALLVRFIKSYFGAPKRDEILVIPGDAENYRSGSK
                     NASSQWIEPGQSFICRGYSIDDGMVYVGERMRDFTGSNEGCLINPELDVAPEGTPSLP
                     VQQIQPRYETMSPEQRGAFLRWLAEGRFGRADPAWPFLFFYGIERRLFVDGPRGYVSH
                     QERNELVLEVRRLLHVYGEHRSFRGYCKNLLATEWVLKGDYAEIPEWVDFTDRFCAEA
                     FNSIISLYVSRDQPIPGEVALQWLFLHPEHIPGTLSRREPEKFEHLFLAGYKEKFGEG
                     ILIHKNRSPLNLIYKGANPSLGNGVKLTVPSLPDPFLLATP"
BASE COUNT          348 a          447 c          489 g          451 t
ORIGIN      
        1 aacgcgctgg ggtattggga ggggaacaga atatacaggg agtaccacgt gtacatcgac
       61 tggcgctggc tgtccatgga attggcaact cttgcggtcg cggtgggact tttatggcgc
      121 tggaagcttc ccttcatgct catgccggtt gcgctgaccc tctggtacat gagcatggac
      181 ctggcgccct tccttttcaa gaatgaggat cttacttggg aactgcgacg gacggtttcg
      241 ctctggtttg gcctcgcagt gctctgtttt gcgatatttg tcgatttccg tacgcgttcg
      301 cgaaaggatt atgcgttttg gctctatctg tgcggcctcg ccgctttctg gggcgggctc
      361 tctctgatgg actcggacag cgaactaggg aagtttgtct acttctgcat caacatcttt
      421 ctgattgccg taggagccct tctctcacga agagcctttg ccgtcttcgg cggatttggg
      481 gccgctgggt atattggtca tttaacatgg agagtgttca gcgacagcgt agtctttccg
      541 ttcgttcttt cgtttgccgg tttttttgtc atctggctcg gaatacgctg gcagaagaag
      601 gaaaaacaga ttacggcgct gctgcgctcc tttctctccc ccgaaatgcg gagttttctt
      661 gagaggaatg agtgattccc tccgagagcg tgacgattcg gagaggcgcc ggccgcgaag
      721 gaatccgcgg aaatctagtg aagggcctgt tgctttgaag ccctgctgaa aagagggaaa
      781 aacgtggagt ttattgtctt tcttgtgatc gccgcacttc tggttcgctt tatcaaatcc
      841 tactttggag ctccgaagcg cgacgaaatt ctggtcattc cgggagacgc ggaaaactac
      901 agaagcggtt cgaagaatgc ctcttcccag tggatcgagc caggacagtc tttcatttgc
      961 agagggtatt cgattgatga tggaatggtg tatgtcggtg aaagaatgcg ggattttacc
     1021 ggctcgaacg aagggtgtct catcaatccg gaactggacg ttgcgcctga gggtaccccc
     1081 tctttaccgg tacagcagat acagccccgt tacgagacga tgagccccga gcagcgcggg
     1141 gcctttctac gctggctcgc agaagggcga ttcggccggg cggaccccgc atggcccttt
     1201 ctcttcttct acggtatcga acggcggctc tttgtggatg gaccgagagg gtacgtctct
     1261 caccaggaac gcaacgagct tgtcctggaa gtgcggcgtc tgctgcatgt ctacggcgag
     1321 caccgttcat tccggggata ctgcaaaaac ctccttgcaa ccgaatgggt cctcaaaggg
     1381 gattatgcgg aaattcccga atgggtcgac ttcaccgatc gtttttgcgc cgaggcgttc
     1441 aactccatca tctcgctcta cgtttcgcgg gatcaaccga ttcccggcga agtggcgctt
     1501 caatggctct ttctgcatcc tgaacatatt ccggggacgc tttcgcgccg tgaaccggag
     1561 aagttcgaac atcttttcct cgcaggatac aaggaaaagt tcggcgaagg catccttatc
     1621 cataagaacc gttcgccctt gaaccttatc tataaaggag cgaacccttc gctgggaaac
     1681 ggtgtcaagc tcacagttcc ttctctaccg gatccctttc ttcttgcaac gccta
//
LOCUS       DAOSWW010000640         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59445_length_1731_cov_3.809666, whole genome shotgun sequence.
ACCESSION   DAOSWW010000640 DAOSWW010000000
VERSION     DAOSWW010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59445_length_1731_cov_3.809666"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PK442_11455"
     CDS             complement(<1..676)
                     /locus_tag="PK442_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOO88311.1"
                     /translation="MKIIVCVKQVPDTNEVRLDPVTGTLMREGVPSIVNPDDKAGLEA
                     ALRLKDQYGAHVTVLSMGPPQADLALRETLAMGADEAVLLTDKAFSGADTWATSSTLA
                     SALSDMEYDLIITGRQAIDGDTAQVGPQIAEHLGLVNISYAEDLRVEDDCVVVKRQFE
                     DRYHIIKAKMPCLITALSEMCEPRYMTPGGIFDAYRSREIKTLVRSDLKIDDDNIGLK
                     GTPTRVV"
     gene            complement(690..>1731)
                     /locus_tag="PK442_11460"
     CDS             complement(690..>1731)
                     /locus_tag="PK442_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOO88312.1"
                     /translation="NERFPSETVEKMARFGFLGIPYGKEWEGQGCDTLTYILCVEEMS
                     RFCATTGVIVSAHTSLGCDPIAKYGTPEQKEKYLPDLLSGRKLGAFALTEPGAGTDAA
                     GQQTKAVLDGENYVLNGSKIFITNAGQADVYIIFAMTDKSLGTKGISAFIVEKEFPGF
                     SVGKKEKKMGIRGSSTCELIFENCLVPKENMLGKEGKGFGIAMGTLDGGRIGIGAQAL
                     GVAQGALDETVAYVKERKQFGKPISAFQNTQFQLAEMQTRIEAARHLVYDAAATKDSG
                     ARFSVEAAMAKLYASQTAMEVTTKCVQLHGGYGYMRDYPVERMMRDAKITEIYEGTSE
                     VQLMVVAGALLK"
BASE COUNT          361 a          524 c          417 g          429 t
ORIGIN      
        1 tgacgactcg cgtaggagtg cctttcaaac cgatattatc atcgtctatt ttcaggtcgg
       61 atcggacgag agttttgatc tcccgtgagc gataggcatc gaagatgcct ccgggcgtca
      121 tgtaacgagg ttcgcacatc tccgaaagcg cagtgataag gcagggcatc ttcgctttaa
      181 taatatgata gcggtcttcg aattgtctct tgacgacgac gcaatcgtct tcaactcgca
      241 aatcctccgc atagcttata ttcacaagcc ccaggtgttc cgctatttgc ggtcccacct
      301 gcgccgtgtc tccatcgatg gcctgtctgc cggtaatgat caaatcgtac tccatatccg
      361 aaagggcgga agcgagagta cttgaagtcg cccatgtatc ggccccgctg aaagccttgt
      421 ccgtcagaag gacggcttcg tcggctccca tagccaatgt ttcgcgcaga gcgagatcgg
      481 cctgaggggg tcccatcgag agaacggtca catgcgctcc gtactggtct ttaagccgca
      541 acgccgcctc cagaccggcc ttgtcgtcgg gattgacgat actgggaacg ccttcgcgca
      601 tcaatgttcc ggtcacggga tccagccgca cttcattggt atccggcacc tgtttcacac
      661 atacaatgat cttcatgcgt ctcacccctt tatttcagca aggcgccggc gacaaccatc
      721 agctgcactt cgctggtacc ttcgtagatc tccgtgatct tcgcgtcgcg catcattctc
      781 tccacggggt agtcgcgcat atatccgtat ccgccgtgaa gctgcacgca tttcgtggta
      841 acttccatag ccgtctgaga ggcataaagc ttggccatcg ccgcctcgac ggaaaatctg
      901 gctccggaat cctttgtcgc tgcggcgtca tagaccagat gtctcgcagc ctcgatccgc
      961 gtttgcattt cggcaagctg gaactgcgtg ttttgaaaag cggagatcgg cttcccgaac
     1021 tgcttccgct ctttgacgta cgccacggtt tcatccaacg ccccctgcgc gactccgagc
     1081 gcctgagctc cgattccgat gcgtcctcca tccaaagtcc ccatcgctat accgaaccct
     1141 ttcccttcct tgccgagcat attctcctta ggaaccaggc aattctcaaa gataagctcg
     1201 cacgttgaag aaccccgtat ccccatcttc ttctctttct tgcctacgga aaaccccggg
     1261 aattccttct cgacgatgaa agccgaaata ccttttgttc cgaggctctt gtcggtcatc
     1321 gcaaagataa tatacacatc ggcctgtccg gcgttggtaa taaatatctt gctgccgttc
     1381 agaacataat tttctccgtc aaggacagcc ttcgtctgtt gcccggccgc atcggtcccc
     1441 gctccgggct ccgtaagcgc aaaagcgccg agctttcttc cggagagaag atcgggaaga
     1501 tatttttctt tttgctcagg ggtaccatac ttggcgatcg gatcgcaccc caacgacgtg
     1561 tgcgcagaaa cgatgactcc tgtcgtcgcg caaaatcggc tcatttcttc aacgcagaga
     1621 atataggtca gagtgtcgca cccctgccct tcccattctt tcccgtacgg gatgccgagg
     1681 aagccgaatc gggccatctt ttccaccgtc tcggaaggaa accgttcgtt t
//
LOCUS       DAOSWW010000641         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59561_length_1729_cov_3.278973, whole genome shotgun sequence.
ACCESSION   DAOSWW010000641 DAOSWW010000000
VERSION     DAOSWW010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59561_length_1729_cov_3.278973"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..483
                     /gene="dut"
                     /locus_tag="PK442_11465"
     CDS             40..483
                     /gene="dut"
                     /locus_tag="PK442_11465"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOO88313.1"
                     /translation="MPVAVKILREGRAKEFPLPEYATESAAGADLRSAEDMLLLPGER
                     TSVGTGIRIELPEGYEAQIRPRSGLAIRHGVTILNAPGTIDSDYRGEIRILLVNLGKD
                     PFTIVAGDRIAQLVVAPVARVEWREDETLGLTGRGEGGFGSTGKS"
     gene            803..>1729
                     /locus_tag="PK442_11470"
     CDS             803..>1729
                     /locus_tag="PK442_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOO88314.1"
                     /translation="MMQFTGNNGKQAELASGTAGDVLDALGMKKGAIAAKIDGCAVDL
                     SRVVERGGEAAPILPESDEGLDILRHSAAHLMAQAVAQMYPGTRYGVGPAIKDGFYYD
                     MEIPGGITEEDLPKIEKEMHRIAKRSVPVERRVLSKAEALEWFKAKNDPYKVEIISEL
                     EDETVTLYVQGEYADLCRGPHVPNTSYVRHFKLLSVAGAYWRGDEKNIMLTRVYGTAF
                     GDEESLAAYIHRMEEAKNRDHRRLGKELDLFSLQAEGPGFPFFHPKGMVILNNLMDFW
                     KKEHTKRGYCEIRTPLILERSLWIQSGHWDHYR"
BASE COUNT          386 a          470 c          572 g          301 t
ORIGIN      
        1 ccccgcaggc actcatcggg aggaaggccg tagcctcgaa tgccggtggc tgtaaaaatt
       61 ctcagggaag gaagagcgaa agagttccct cttcctgaat acgcaacgga gagcgctgca
      121 ggcgcggatc ttcgctcggc ggaggacatg ctcctcctgc cgggggagcg gacgagcgtc
      181 ggcaccggca tccggataga gcttcccgaa gggtacgaag cgcagattcg acccaggagc
      241 ggtctggcta ttcgtcacgg tgtgacgatc ctgaacgctc ccggaacgat cgacagcgat
      301 tacagggggg agatcaggat cctcctggtg aacctcggaa aagacccctt caccatcgtc
      361 gcgggcgaca gaatcgcgca gcttgtcgtc gctcccgtcg ccagagttga atggcgcgag
      421 gatgaaacgc tcgggctgac gggacgggga gagggcggtt tcgggagcac cggaaagagt
      481 tgacagattc cccttttagg ggtataagtt cagaaaagtt ttctttcctg cgatgagagg
      541 gagagtaggg acgattcgtc cggacagaga gggaggcacg cggctgaaag gcctcctccg
      601 gattcgtcat cgaacaccgc ccttgagctg acgccgaaat acccctcggg acaaggcgtc
      661 tggggatccg ccccgaacag cggacacgag cgccgcgcaa aagcagttcg cgcggaaatg
      721 gagtggaacc gcgacatgcc cacgtcgtct ccataatgga gatgcgtggg cattttttta
      781 tcgaaaacga ggaggtagga gtatgatgca gtttacaggg aacaacggca aacaggcgga
      841 gttggcttcc ggcacggcgg gcgatgtgct cgacgcgttg gggatgaaga agggcgcgat
      901 agcggcgaag atcgacggct gtgcggtgga tctctcgcgc gtcgtcgaaa ggggcggcga
      961 ggctgccccg atacttccgg agtcggacga ggggctcgac attctgcggc actccgcagc
     1021 gcatttgatg gcgcaggccg tcgcccagat gtacccggga acgcgctacg gcgtcggccc
     1081 ggcgatcaag gacggttttt attacgacat ggagatcccc ggaggcatca ccgaggagga
     1141 tctgccgaag atcgaaaagg agatgcaccg gattgccaag cgctccgttc ccgtggaacg
     1201 gcgggtgctg agcaaagccg aagcgcttga atggttcaag gcgaagaacg acccgtacaa
     1261 ggtagagatc atctccgagc tggaagatga gacggtcacg ctctacgttc agggtgaata
     1321 cgcggatctc tgccgggggc cgcacgtccc caatacctcc tatgtccgtc acttcaagct
     1381 gctctccgtg gcgggggcgt actggcgggg cgacgagaag aacatcatgc tcaccagagt
     1441 ctacggaacg gccttcggcg atgaggagtc cttggcggcg tacatccaca ggatggagga
     1501 ggcgaagaac cgcgaccaca gacgtctcgg caaggagctc gacctcttca gcctccaggc
     1561 cgaggggccc ggtttcccgt tctttcaccc gaagggtatg gttattctca acaacctgat
     1621 ggatttctgg aagaaggagc acacaaagag agggtactgc gagatccgca ccccgcttat
     1681 cctcgaacgc tcgctttgga ttcagtccgg ccactgggat cactatagg
//
LOCUS       DAOSWW010000642         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59562_length_1729_cov_3.270609, whole genome shotgun sequence.
ACCESSION   DAOSWW010000642 DAOSWW010000000
VERSION     DAOSWW010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59562_length_1729_cov_3.270609"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK442_11475"
     CDS             <1..415
                     /locus_tag="PK442_11475"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049217.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOO88315.1"
                     /translation="FESGLTEKEVLRKVVGKGGLAAYTGTSDVREVKALIAEGDEAAR
                     RAYEAMAYSVAREIGAMAAALSGKVDAILITGGVAYDNDFVALVQKRVQWIAPVLIYP
                     GEDEMPALAEGAFRVLQGEELQKVYSEYISGGDRS"
     gene            412..1320
                     /locus_tag="PK442_11480"
     CDS             412..1320
                     /locus_tag="PK442_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOO88316.1"
                     /translation="MTMHGIDFLLAESKKGRSMTLALACPYGDDALGAACRAQREGIV
                     KTILIGDEGKIRTLAGRDGYDLSDIAIVDEKNDDTAVERSVKMVSSGEADLLMKGLVK
                     TSSLLKAVLDKEWGLRTGSLLSHLFLFEVPTLGGRVIGISDGGMNTYPDLNAKVRIIE
                     NAVACYHRIGVPQPKIAALAAVEVVNPDMLATLEAAALAKMNERGQIKGCLVDGPFAL
                     DNAVSEESARIKGISSPVAGNADMLLVPDIESGNFVGKVLLYMTGGKGAGVILGARKP
                     IVLTSRFDSMETKLLSIALGAVVARS"
     gene            1356..1502
                     /locus_tag="PK442_11485"
                     /pseudo
     CDS             1356..1502
                     /locus_tag="PK442_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
     gene            complement(1554..1691)
                     /locus_tag="PK442_11490"
                     /pseudo
     CDS             complement(1554..1691)
                     /locus_tag="PK442_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
BASE COUNT          338 a          503 c          585 g          303 t
ORIGIN      
        1 cttcgagagc ggcctcacgg agaaggaggt tctccgcaaa gtcgtgggga agggaggtct
       61 tgccgcctac accggcacga gcgacgtccg cgaggtgaag gcgctgatcg ccgaaggaga
      121 cgaggccgcg aggcgcgcat acgaggccat ggcgtactcg gtcgcccgcg agatcggcgc
      181 gatggccgcc gcgctctccg gaaaggtcga cgccattctc atcaccggcg gcgtggcgta
      241 cgacaacgat ttcgtcgcgc tcgtacagaa gcgcgtccag tggatcgccc ccgtgcttat
      301 ctatccgggc gaggacgaga tgcccgcgct cgcggaaggg gcgttcagag tcctccaggg
      361 cgaggagctg cagaaagtat attccgagta catctcagga ggcgatcgtt catgaccatg
      421 cacggtatcg attttcttct tgcggagagt aaaaaagggc gctccatgac gcttgcgctc
      481 gcctgtccct atggtgacga cgcgttgggc gcggcgtgca gggcgcagcg tgaagggatc
      541 gtcaagacga tcctcatcgg cgacgagggg aagatacgca ccctcgcggg gcgagacggc
      601 tacgatctct cggatatcgc aatcgtcgac gagaaaaacg acgacacagc ggtggagcgg
      661 agcgtcaaga tggtctcctc cggggaggcc gacctgctga tgaaggggct cgtgaagacg
      721 tcgtccctgc tgaaggcggt gctcgacaag gagtgggggc tacggaccgg ttcgctgctc
      781 tcgcatctct tcctcttcga agtgcccacg ctcggaggcc gggtgatcgg catctccgac
      841 ggcggcatga acacctaccc cgacctgaac gcgaaggtaa ggattatcga gaacgcggtt
      901 gcctgctacc acaggatcgg cgtcccgcag ccgaagatcg ccgcgctcgc cgccgtcgag
      961 gtggtgaacc ccgacatgct cgcaacgctt gaagccgccg cgcttgccaa gatgaacgaa
     1021 cgagggcaga tcaaggggtg cctggtcgac ggtccctttg cgctggacaa cgcggtcagc
     1081 gaggagtccg cgcgcatcaa gggaatctcc tcgcccgtgg ccggcaacgc cgacatgctg
     1141 ctggttcccg acatcgagtc gggcaacttc gtcggcaagg tgctgctcta catgacgggc
     1201 ggcaaggggg cgggcgtcat cctcggcgcg cgcaagccca tcgtcctcac cagccgcttc
     1261 gacagcatgg agacaaaact gctttcaatc gccctgggcg cggtcgtcgc ccggagctaa
     1321 aaaagaatat ttcattcaga ggaggagttt ttgttatgga acagattcgc tcgctttccc
     1381 agcttttgga ctacgcaaag aagatcggcg ccgagaaggg gccgaagaag ctctcggtcg
     1441 caatggccga ggaggcgggt ttgctgactg ccatcgaaga ggcgcgcaag gcgggcgtgg
     1501 ccacccaact gggccatgcc ggcctcgaag ctggcgcggg tgcgggtgct ggagaagaag
     1561 aacagcatgg ccagcacttt gtcgcgcaga taggcgtggg gttcgcccag ggcgcgcttg
     1621 cgcttcaggt ccagcgccac gtcgatgatg gtgtcaatct cgtttcgggt ccattcttgg
     1681 gtcgtgatca tgtcacgccc gcgtagatag gtttgcatgg gatgctccg
//
LOCUS       DAOSWW010000643         1728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59621_length_1728_cov_3.219366, whole genome shotgun sequence.
ACCESSION   DAOSWW010000643 DAOSWW010000000
VERSION     DAOSWW010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59621_length_1728_cov_3.219366"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PK442_11495"
     CDS             <1..215
                     /locus_tag="PK442_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88317.1"
                     /translation="ETTDDHVQNLAASMEETAAAMENVATGSQEVSETAEDLKRMTER
                     FKVEKDEDWSNYNEGAGTRKLALSAN"
     gene            264..>1728
                     /locus_tag="PK442_11500"
     CDS             264..>1728
                     /locus_tag="PK442_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO88318.1"
                     /translation="MRWFGNMKVRGKILFLVVLMIVLLGAVGYTGYHFNTKAAQGIEG
                     MYEDRLLPVSWLIDTIANAALIKGNVYALMLTADDRENESLNAEIGAWAARNDENLNN
                     YSKTDLLDYEKDHLATARQTLQEWRKGWGKTRELAMANKNQEAYAQFTQEALPHLVRF
                     QEEINGLADFNRKTAEEINAQNTSDTKTALYMIVGIVLAAVVLALLFGLFIASRISAP
                     LRTVVELAGRASNGDLTIGREEFGIATRDELGIMADALAEMVAKQREAMRSIAASAEK
                     LGSNAEEFAALAEESNAGVEESRAGVDDVSSQMESLAASSEEINASVEEVASGAQSSA
                     QKGTEMANEVEQARLAGEEGTKAVEKAVASINGVAKESERSAQQVKSLGDRAREIQSF
                     VTQIGGIADQTNLLALNAAIEAARAGEAGRGFAVVAEEVRKLAEESNEAAKQIAALAA
                     GITKDLDSVVTSSEQGAKASKDSSHLAEETRTTIGKMM"
BASE COUNT          416 a          482 c          562 g          268 t
ORIGIN      
        1 tggagaccac ggacgaccac gtgcagaacc tcgccgcgtc gatggaggag accgcggctg
       61 cgatggagaa tgtggccacg ggatcgcagg aggtcagcga gaccgccgag gatctgaagc
      121 ggatgacgga gcggttcaag gtcgaaaagg acgaggactg gtcgaactac aacgagggag
      181 ccggaacaag gaaactcgcg ctctccgcga actgaatccg gcggctcttc gccgaaaaaa
      241 cacaacgaaa ggaagcgatt tgcatgcgtt ggttcggaaa catgaaggtc cgcggcaaga
      301 tactgttcct ggtcgttctt atgattgtac tgctcggggc ggtcggctac acggggtacc
      361 acttcaacac gaaagcggcg cagggtatcg aaggaatgta tgaagaccgt cttctccccg
      421 tttcttggct tattgatacg atcgcaaacg ccgctctgat caagggtaat gtctacgctc
      481 ttatgcttac agcagatgat cgggagaacg aatctttgaa cgccgagatc ggggcatggg
      541 cagcgcggaa cgacgaaaat cttaataatt actcgaaaac agatcttctt gactatgaaa
      601 aagaccatct ggccacagcc cggcaaactc ttcaagagtg gagaaagggg tggggaaaga
      661 ctcgcgaact ggccatggcg aacaagaatc aggaagcgta cgcccagttc acccaagagg
      721 cgctccccca ccttgttcgc ttccaagagg agattaacgg tctggctgac ttcaatcgga
      781 aaaccgcaga ggaaatcaac gcacaaaaca cctccgatac aaaaaccgcc ctctacatga
      841 tcgtcggcat cgtactcgcc gccgttgtgc tcgcgcttct cttcggcctc ttcatcgcat
      901 cgcgcatttc cgcgcctctg cggacggttg tcgagctcgc cggacgggcc agcaacggcg
      961 acctcaccat agggcgcgag gagttcggta tcgccacccg cgacgagctc gggataatgg
     1021 ccgacgcgct cgccgagatg gtggcgaaac agcgcgaggc gatgcgctcc attgcggcat
     1081 ccgcagaaaa gcttggaagc aacgccgagg agttcgcagc cctcgccgag gagtcgaacg
     1141 ccggggttga agagtcgcgc gccggtgtcg acgacgtctc ctcccagatg gagagtttgg
     1201 ccgcgtccag cgaagagatc aacgcctccg tggaggaagt ggcgagcggc gcccagtcct
     1261 ccgcgcagaa ggggaccgag atggcgaacg aggtggaaca ggcgcggctc gcgggcgaag
     1321 agggaacgaa agctgtcgag aaagccgtcg ccagcatcaa cggcgtggcg aaggagtcag
     1381 aacgttccgc gcagcaggtg aagagtttgg gcgaccgggc gcgggagatc cagagcttcg
     1441 tcacccagat cggcggcatc gcggaccaga cgaatctgct ggcgctgaac gccgccatcg
     1501 aagcggcccg ggccggcgag gccggacgcg gcttcgcggt cgtcgccgaa gaagtccgca
     1561 agctggccga ggagagcaac gaggcggcga agcagatcgc ggcgcttgcc gcaggaatca
     1621 cgaaggatct ggacagcgtc gtcacgtcgt cggaacaggg agcgaaggcg tcgaaggact
     1681 ccagtcacct cgccgaggaa acgcgcacga ccatcgggaa gatgatgg
//
LOCUS       DAOSWW010000644         1727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59682_length_1727_cov_3.816986, whole genome shotgun sequence.
ACCESSION   DAOSWW010000644 DAOSWW010000000
VERSION     DAOSWW010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59682_length_1727_cov_3.816986"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..263)
                     /locus_tag="PK442_11505"
     CDS             complement(57..263)
                     /locus_tag="PK442_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88319.1"
                     /translation="MAGKYGFTAATETEREVFRRNDRHLDDMFSVSWRGPFEGFSASR
                     KADLAEPSFSQTEMRWRGPFEGLR"
     gene            730..>1727
                     /locus_tag="PK442_11510"
     CDS             730..>1727
                     /locus_tag="PK442_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOO88320.1"
                     /translation="MVNGFFVFFVFAAVYLGMIFGRFPRLALDRTGIVLLGAIVLLAA
                     GKASLEGGRSGIDVPTILLLFGFMVISAQFRLGGFYSEVSRRIGALAAPPSLIMGAVI
                     GASALMSALLTNDVVCLAMTPVLGAGCIRRGLNPLPFFLALACAANIGSALTLIGNPQ
                     NMLIGQYLSLPFGGYLLFAAFPVFLSLLSLWGIMLFLFRGRWTLQMDEQLVDERSFDA
                     WQTLKGGVVLTVVVGLFLFSTLPRDLTALGAAGVLLLSRKMASRRTLEIVDWQLLLLF
                     LGLFVVNAAFESAGGLQILKEFLESAGVSLSSPAWLYGVTTLLSNLVSNVPAVMLLL"
BASE COUNT          299 a          485 c          465 g          478 t
ORIGIN      
        1 ggaagggatg acaggatcgg gggacgcttc cgtcattttc ccgcttcttt ttaagcctag
       61 cgaagtcctt cgaacgggcc gcgccaccgc atctccgtct gtgagaagga gggttccgca
      121 agatcggcct tccttgaagc ggagaatcct tcaaagggcc ctctccaact gaccgagaac
      181 atatcgtcga gatgacgatc atttcttctg aagacctctc tctctgtttc cgtcgccgct
      241 gtgaatccgt actttcctgc cattgtacac actcccttca aaattataaa aagacgcaga
      301 gtaaaaacac ttcccaataa aaaaaccggg ggcctccaga aaggcggccc ccggttccgg
      361 ttttaggtgt acggcttatg cgccgaaccc accggaaggg ggaccgccga taatgaggag
      421 tacgaggacg agtacaagaa cagaaatacg aaccaaactc aatctctgtg cattcatcaa
      481 aatggcctcc tttccggtga agtgtctgtc tgaatattga tagggatgat acagcccctg
      541 cggtatcctg tcaagcatct atttccagac tttttacgta aatttacgtt cccggggtgc
      601 aaaagtagga tttgggatga aaaatgtggg tacatccgga acgacacgct cattccaagc
      661 tttccagagc acgctgttcg ggagtataat tccctcttgt tgtttactta tttctgcgag
      721 gtgtttcctg tggtgaatgg gttcttcgtc ttttttgttt ttgccgccgt ctatctgggg
      781 atgatctttg ggcgctttcc tcgcctcgcg cttgaccgca ccggtattgt ccttttgggc
      841 gcaatcgtgc ttcttgccgc gggaaaggcg tctctcgagg gcggccgctc cgggatcgac
      901 gtccccacaa ttttgctctt gttcggcttt atggttattt cggcgcagtt ccgtctggga
      961 ggtttctact ccgaggtttc ccggcgcata ggggctctcg cggcgcctcc ttcgcttatc
     1021 atgggggcgg tcatcggagc ttccgctctc atgtcggcgc tcctcacgaa cgatgtggtc
     1081 tgtctcgcca tgacgcccgt tctgggggcg gggtgcatcc gccgggggct caaccctctt
     1141 ccttttttcc ttgcgcttgc gtgcgccgcg aatatcggtt ccgcgctcac gctcatcgga
     1201 aatccgcaga acatgctcat cggccagtat ctctcgcttc ctttcggcgg atatctgctc
     1261 ttcgccgcct tccccgtgtt cctgtcgctg ctttcactct gggggatcat gctcttcctt
     1321 ttccggggac gctggacgct ccaaatggat gagcagctgg ttgatgagcg ctcctttgat
     1381 gcctggcaga cgttgaaggg gggtgtcgtc ctgacggtag tcgtgggctt gtttctcttc
     1441 tcgacgcttc ctcgtgatct cacggcgctc ggcgctgcgg gggtccttct gctcagcagg
     1501 aagatggcgt cgcggcggac cctcgaaata gtggactggc agcttcttct ccttttcctc
     1561 ggccttttcg tcgtcaatgc ggcgttcgag tctgccggag gtcttcagat tctcaaagag
     1621 ttccttgaat cagcaggggt atcgctttcc tctccggcct ggctttacgg agtgacgacc
     1681 ttgctctcga atcttgtctc caatgttccg gctgttatgc tgctgct
//
LOCUS       DAOSWW010000645         1727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59702_length_1727_cov_2.647129, whole genome shotgun sequence.
ACCESSION   DAOSWW010000645 DAOSWW010000000
VERSION     DAOSWW010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59702_length_1727_cov_2.647129"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..550
                     /locus_tag="PK442_11515"
                     /pseudo
     CDS             1..550
                     /locus_tag="PK442_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712421.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
     gene            640..>1727
                     /locus_tag="PK442_11520"
     CDS             640..>1727
                     /locus_tag="PK442_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOO88321.1"
                     /translation="MKCRNESATPEQRAVPNAEAVQPALFEYGDGMWPLLSNVKRSSR
                     YAGCEYGVIPPKDGNSIVRFCLAFPDVYEIGMSYLGFQILYFLLKNLPCGDADRVYCP
                     WTDLEEQMRARETPLTSLESGRPLREFDVVGFTLQYELSYTNILTMLDLGRIPLLSAD
                     RGENDPLVAAGGVGALSPEPLAPFIDFFCVGDGEDLLPEVITLLSGAKGLSRREKLEL
                     LSGIEGVYVPSLVECDVGEGGVSFRSAAEKRVALPVRRRIVESLDDAFFPEMLLVPSG
                     GIVHDRVAVELFRGCTRGCRFCQAGMVTRPVRERSVRPLAERIVSLVEKTGWEEVGLL
                     SLASCDYSGIGELVRELSPMLAEKNVKL"
BASE COUNT          287 a          513 c          539 g          388 t
ORIGIN      
        1 ggccgggctg cttgctctct tcacagcgct tctgccgttc ggatggcgtt ttttacgata
       61 gtatcagaag atgcggatac gcgtctttct cgatccgtgg atcgacccgc tcggcgccgg
      121 gtacaacgtt atccagtcga ggatagccgt tggttccggc gggctgttcg gcgccggcta
      181 tatggagggg ctgcaaagca agctcaagtt cttgccggaa ccgcataccg acttcatctt
      241 tggcgtcttc tcggaggaat tcggttttat cggtgcgctg gtgatgcttg gactcttcgc
      301 ccttctgttc tggcgactcg tggacgccgg gctgcgcagc aaggacggga gggccaaggt
      361 tcttatcgcc gggatttcaa cctggttctg gtttcagatg ctgcaaagca tcggcatgag
      421 catggggctc ctgcccataa cgggccttcc ccttccgctc gtcagttacg ggggcagttc
      481 gctgctctcg ttttccgttg cgctcggact tgcccagagc gtgtacgttt cgacgctgaa
      541 aagatactga gcatcatctc cgcgccggga aaaccggcgc ggaaacatga gaaatcctgg
      601 tgtctgcagg tgtgtatatc gagggaggaa ttgtaatcta tgaagtgcag gaacgaatcc
      661 gcaacgccgg agcagcgtgc cgtcccgaac gcagaagccg ttcagccggc tctcttcgag
      721 tacggcgacg ggatgtggcc gctcctctcc aatgtgaagc gctcctcccg gtacgcgggg
      781 tgcgagtacg gcgtcatacc tcccaaggac gggaattcca ttgtgcgttt ctgcctcgct
      841 ttccccgatg tgtacgagat aggaatgagc tatctcggat tccagatcct ctacttcctc
      901 ctgaagaatc tcccgtgcgg cgacgcggac cgggtctact gcccgtggac cgacctcgag
      961 gagcagatgc gggcgcggga gacgccgctg acctcccttg aaagcggccg tcctctccgc
     1021 gagttcgacg tggtcggttt caccttgcag tatgaactga gctacaccaa catcctcacc
     1081 atgctcgacc ttgggcgtat cccgctgctt tcggccgaca ggggcgagaa tgaccccctc
     1141 gtcgcggcgg gaggcgtcgg cgcgctctcc ccggaaccgc tcgctccgtt tatcgacttc
     1201 ttctgcgtcg gcgacggaga ggatctgctt ccggaggtga tcacgcttct ttccggcgcc
     1261 aaggggctct cccggagaga aaaacttgag ttgctctccg ggatagaagg ggtgtacgtt
     1321 ccctctcttg tcgaatgcga cgtcggcgag ggaggcgtca gtttccgttc tgcggcggag
     1381 aagcgcgtcg cgctccccgt tcggcggcgc atcgtcgaat cgctggacga cgcctttttc
     1441 ccggagatgc tcctcgtgcc gtcaggagga attgtccacg accgtgttgc cgtggaactc
     1501 ttccgcggct gtaccagggg ctgccgtttc tgccaggcgg gcatggtgac ccgtcccgtc
     1561 agggagcgct ccgtccgtcc cctcgcggag cgaatcgttt cgctggtcga gaagacgggg
     1621 tgggaggagg ttggtctcct ttccctcgca tcctgcgact actccggcat cggcgaactc
     1681 gtcagggagc tctcgccgat gctggcggaa aagaacgtca aactgag
//
LOCUS       DAOSWW010000646         1725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59806_length_1725_cov_2.549102, whole genome shotgun sequence.
ACCESSION   DAOSWW010000646 DAOSWW010000000
VERSION     DAOSWW010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59806_length_1725_cov_2.549102"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PK442_11525"
     CDS             complement(<1..1112)
                     /locus_tag="PK442_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO88322.1"
                     /translation="MNMKPGKGFADFFGLKKSIVGMLSMVILVGLGERMAERFLPIYL
                     LTLGGGALSIGMLNGLDNLLSALYSFPGGYIADRFGTKRALLFFNILAMTGFAVVIFV
                     PRWEAVIGASFLFLSWTAISLPATMKLIASALPKNKRTMGVSVHSLVRRIPMALGPIV
                     GGAMIGLFGESLGVRIAFGGAFFFALLALLLQQRLIDDAHQDVVERPEGNPLKIFRKM
                     SPELKNLLVSDILVRFCEQIPYAFAVVWAMKTIASPVTAFQFGLLTAVEMVVALLIYI
                     PVAWLADRGRKKIFVTITFCFFTAFPLALLASRSFEALLIAFAVRGLKEFGEPTRKSL
                     ILDLSPQGESAAMFGLYYLLRDTVVSVAAFGGAFLW"
     gene            complement(1109..>1725)
                     /locus_tag="PK442_11530"
     CDS             complement(1109..>1725)
                     /locus_tag="PK442_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOO88323.1"
                     /translation="CLAPLVELANDRDLWINENPDSRLWQALITLCGPQSLFARLAAN
                     PEAVLLPHERAAAVDFVEKQEKRFALAVEKIGKSKDDLAFVEPGILEFGDVSDFGGLV
                     LDRMANPPLLVAVAAKRYSGEWAVSMRSRSETAGKVVGMLRDGKKIRGGGHDDSAALY
                     FPPSYSPEQIRTTITAALRTIKDSERPTGVTLGDLLASGKSGSR"
BASE COUNT          342 a          544 c          574 g          265 t
ORIGIN      
        1 atccagagga acgcgccgcc gaaggccgcc acggagacta cggtatcccg cagcaggtag
       61 tagaggccga acatggcggc ggactccccc tgaggggaga gatcaaggat cagcgatttc
      121 ctcgtaggtt cgccgaactc cttcagtccg cgcaccgcga aggcgatcag gagcgcctcg
      181 aaggagcgcg acgcgaggag cgccagagga aaggccgtaa agaagcagaa ggtgatggtc
      241 acgaagatct tcttccgccc cctgtcggcc agccaggcca cggggatgta gatcagcagg
      301 gcgacgacca tctcgaccgc cgtgagcagt ccgaactgga aggccgtcac gggagaggcg
      361 atcgtcttca tcgcccagac gacggcgaac gcgtagggga tctgctcgca gaagcggacc
      421 agaatatcgg agacaagcag attcttcagc tcggggctca tcttccggaa tatcttcagc
      481 gggttcccct cgggccgctc gacgacgtcc tgatgcgcgt cgtcgatgag gcgctgctgg
      541 agcagaagcg cgagcagcgc gaagaaaaac gcgcctccga aggcgattct gacgccgagc
      601 gactccccga acaggccgat catcgcgccg cctacaatag gcccgagcgc catgggaatc
      661 cggcgtacaa gggagtggac ggagactccc atggtgcgct tgttcttggg cagcgccgag
      721 gcgatgagct tcatcgtcgc cgggagggag attgccgtcc acgagaggaa caggaaagag
      781 gcccctatga ccgcctccca gcggggaacg aagatcacca cggcaaaccc cgtcatcgca
      841 agaatgttga agaagagcag cgctcgcttc gtgccgaagc ggtcggcgat ataccctccg
      901 gggaaggagt agagcgccga gaggagattg tccagcccgt tcagcatgcc gatggagagc
      961 gcgcctcccc cgagcgtgag aaggtagatc gggaggaaac gctccgccat ccgctctccc
     1021 aggcccacga ggatcaccat ggagagcatg ccgacgatgc tttttttcag gccgaagaag
     1081 tcggcaaacc ccttccccgg tttcatgttc atctgcttcc cgattttccc gaggccagca
     1141 gatcgcccag cgtcacgccg gtggggcgct ccgagtcctt gatcgtccgg agggccgccg
     1201 tgatcgtcgt gcggatctgc tcgggagagt aggagggggg gaagtagagc gccgcggagt
     1261 cgtcgtgccc gcccccccgg atcttcttgc cgtcgcggag catcccgacc accttacccg
     1321 ccgtctcgct ccggctgcgc atcgacacgg cccactcgcc ggagtagcgc ttcgccgcca
     1381 ccgcgacaag cagcggcggg ttcgccatcc ggtcgagcac gaggccgccg aagtcggaga
     1441 cgtcgccgaa ctcgaggatg cccggctcga cgaaggcgag gtcgtctttg ctcttgccga
     1501 tcttctccac cgcgagggcg aagcgcttct cctgcttctc gacgaagtcc acggcggctg
     1561 cccgctcgtg cggaaggagg acggcctcgg ggttcgcagc cagccgcgcg aagaggctct
     1621 gcgggccgca gagggtgatg agcgcctgcc agagacggct gtccggattc tcgttgatcc
     1681 agagatcccg gtcgttcgcc agctccacga ggggggcgag gcatt
//
LOCUS       DAOSWW010000647         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59911_length_1723_cov_3.239209, whole genome shotgun sequence.
ACCESSION   DAOSWW010000647 DAOSWW010000000
VERSION     DAOSWW010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59911_length_1723_cov_3.239209"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PK442_11535"
     CDS             complement(<1..681)
                     /locus_tag="PK442_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO88324.1"
                     /translation="MLSPVYAPYTLVVERAEGVRLFTDKGVFLDTYAGIGVLPLGHSH
                     PDVVRAIREKSERYTHLSNYFLDPDAPALAESLLFMTGRKGEVYFANSGAEAMEAALK
                     AVKKNREGALVSFEGNFHGRTLGALSVTWGPAIRHPFDPLIPHCAFLPFNGEALLRFA
                     RENEIAAVFLECIQGNSGVYPLPADLAEAVNILQKERGILVVADEIQAGLGRTGRYFA
                     YEHFGLRPD"
     gene            complement(686..>1723)
                     /locus_tag="PK442_11540"
     CDS             complement(686..>1723)
                     /locus_tag="PK442_11540"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOO88325.1"
                     /translation="EPSPRELDMLLATGEQMTSALLAMALSDFGIPALSYNAFQLELT
                     TTGDFSNARIMDLNLNKLKRQLEMRSVIVVTGFQGITPDGDLTTLGRGGSDTSAVAIA
                     AKCGCPCEIYSDVAGVYTCDPNKRAGARKLEYITFDEMLELSSLGAKVLHSRAVEIAK
                     KYSVDLYCASTFSDERGTYVVNSLPEWLEQPVVTGVTTDSNQMRVNIVRMPASMDAVS
                     ALFGGLAEQGVNVDMISTVNENGHSHLTFTVVDAKEKTVLETVRRTLAPWEGWNVTED
                     RNVAKISAVGVGMKSAPGVAARFFGALSRKGIKMLGTTTSEIKISALVSREEGDEALR
                     ALVDEFGLGEA"
BASE COUNT          326 a          558 c          510 g          329 t
ORIGIN      
        1 atcggggcga aggccgaagt gctcgtaagc gaagtacctg cccgtgcgcc caagacccgc
       61 ttgaatctcg tcggccacca ccaggatgcc ccgctctttc tgcaggatgt tcaccgcctc
      121 ggccaggtcc gcgggaagcg gatagacgcc gctgttcccc tggatgcact ccaggaagac
      181 ggccgcgatc tcgttctccc gcgcgaacct cagcagcgcc tccccgttga acgggagaaa
      241 ggcgcagtgc ggaatgagcg gatcgaaagg atggcgtatc gcaggccccc aggtcactga
      301 gagcgcaccc aaggtacgcc cgtgaaagtt tccctcgaaa gagacgagcg ccccttcgcg
      361 gttcttcttc accgctttca gagcggcttc catcgcttcg gcgccggagt tggcaaaata
      421 cacctcgcct tttctgccgg tcatgaagag cagcgattcg gcaagcgcag gcgcgtccgg
      481 gtcgagaaag tagttcgaca gatgcgtata gcgttccgat ttctcacgga ttgcccggac
      541 gacatccggg tggctgtgtc cgagagggag gacgccaatg cccgcgtacg tatccaggaa
      601 gacgccctta tcagtgaaga gccggacgcc ttccgcccgc tcgacgacaa gagtgtacgg
      661 cgcatagacg ggggagagca tccggctatg cctcccccag gccgaactca tccaccaggg
      721 cgcgcagcgc ctcgtcgccc tcttcgcggg agacgagcgc agagatttta atctccgacg
      781 tcgtagtccc cagcatcttg atccccttcc gggagagcgc gccgaagaaa cgcgccgcga
      841 cgcccggggc cgacttcatg ccgacgccga ccgcggagat cttcgccaca tttcggtctt
      901 ctgtgacgtt ccacccctcc caaggggcga gcgtcctccg gaccgtttcc agcacagtct
      961 tctccttggc gtccaccaca gtgaaggtga ggtgcgagtg gccgttttca ttgacagtgg
     1021 agatcatatc cacgttcact ccctgctcgg cgagcccgcc gaagagcgcg ctcacggcat
     1081 ccatggaggc cggcatgcgt acgatgttca cccgcatctg gttgctgtcc gtggtcactc
     1141 cggtcaccac aggctgttcc agccattcgg gaagactgtt caccacgtag gtcccccttt
     1201 catcggagaa ggtcgacgcg caataaagat cgaccgaata tttttttgca atttccaccg
     1261 cccgggagtg gagcaccttc gctcccagcg aggagagctc gagcatctcg tcgaaggtga
     1321 tgtactccag ctttctcgcc ccggcgcgtt tgttcgggtc gcaggtgtac acaccggcca
     1381 catcgctgta gatctcgcat ggacaaccgc acttcgccgc gatggcaacc gccgaggtgt
     1441 cggaccctcc ccggccaagc gttgtaagat ccccgtccgg ggttatcccc tggaaaccgg
     1501 tgacgacgat gacgctccgc atttcgagtt gccgcttcag cttgttcagg ttcaagtcca
     1561 tgatccgggc gttgctgaaa tcgccggtcg tggtcagctc aagctggaac gcgttgtagg
     1621 agagcgccgg aattccaaag tcgctcaacg ccatcgccag aagggcggag gtcatctgct
     1681 ctcccgttgc gaggagcatg tccagctcgc ggggcgacgg ctc
//
LOCUS       DAOSWW010000648         1722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_59980_length_1722_cov_2.907019, whole genome shotgun sequence.
ACCESSION   DAOSWW010000648 DAOSWW010000000
VERSION     DAOSWW010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59980_length_1722_cov_2.907019"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PK442_11545"
     CDS             <1..268
                     /locus_tag="PK442_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOO88326.1"
                     /translation="LGLFANPAGLIKNGDTSFRESSNSGLAQVESPQQGGAGSIIGST
                     LEMSNVDLTEEFTRLITAQRGFQANARMITTSDQVLEELINIKR"
     gene            288..515
                     /locus_tag="PK442_11550"
     CDS             288..515
                     /locus_tag="PK442_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOO88327.1"
                     /translation="MITLRRINGERFTINADLIETVESTPDTVIRLMNGHRYVVAEPM
                     DEIVDLVVQYRRQVFFSFIKGNTLVPEEGEE"
     gene            570..1364
                     /locus_tag="PK442_11555"
     CDS             570..1364
                     /locus_tag="PK442_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOO88328.1"
                     /translation="MDLTTIIGLLVCYILVIGGVMAGGQGIAFIDFQSILIVGGGTIG
                     ALVTSNPPEKLKGFMKVLKMAFTGGSPDTVSLVQTIVSFAEKARREGLLALEADAAEL
                     DNEFLRKSIQLVVDGTDPELVKGILDTEIGILETRHSSNKAFFDSMAELAPAFGMLGT
                     LIGLITMLGNLQDPNALGPGMAVALVTTFYGSLIANGFAIPIAKKLASRSSQEVLSME
                     LMVEGVLSIQAGENPRIVEEKLKVFLPPSLRLALEEKAKAAKEGGA"
     gene            1365..>1722
                     /locus_tag="PK442_11560"
     CDS             1365..>1722
                     /locus_tag="PK442_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOO88329.1"
                     /translation="MARSKKKKEDSSPGAPLWLVTYGDMVTLVLTFFVLLFSMSSIDV
                     QKFELMLLSFKGAIGFMPGGKAIQEDSAVFGGQPAQHAGESARVTPEIQQIARNLRNY
                     LRTEGLDKEIVVRIDQR"
BASE COUNT          367 a          465 c          524 g          366 t
ORIGIN      
        1 gctcgggctc ttcgccaacc cggcggggct gatcaagaac ggcgatactt cgttccgcga
       61 aagcagcaac tccggactgg ctcaggttga atcgccgcag cagggcgggg ccggttccat
      121 catcggcagc acgctcgaga tgtcgaacgt ggacctcacc gaggagttca ccaggctgat
      181 cacggcgcag agaggcttcc aggcgaacgc gcggatgatc accaccagcg atcaggtgct
      241 cgaagagctg atcaatatca agcggtaagc gcggggctag ctgaacgatg attacactga
      301 ggcggatcaa cggcgagcgt ttcaccatca acgccgatct cattgagacg gtggagtcga
      361 cgcccgatac ggtgatccgc ctcatgaacg gtcaccgata tgtcgtcgcc gaacctatgg
      421 acgaaatcgt ggatttggtg gtacagtaca ggcggcaggt tttcttctcc ttcatcaaag
      481 gaaataccct cgttccggag gaaggggagg aataagaaaa gcgtcgtgtt tcgacgaccg
      541 acgccgtact tgtgaggggc gggatatttt tggatcttac tactatcatc ggtcttctcg
      601 tatgttacat cctggtcatc ggcggagtta tggcgggagg ccagggaatt gcgttcatcg
      661 attttcaatc cattctcatc gtgggcggcg gtaccatcgg cgcccttgtt acatcgaatc
      721 cgcccgagaa actgaaaggg tttatgaagg tgctcaaaat ggccttcacc ggcggttctc
      781 cggatacggt gtcgctggtg cagaccatcg tcagttttgc ggagaaggcc cggagggaag
      841 gacttcttgc tttggaggcg gacgcggcgg agctggataa cgaattcctc aggaagtcta
      901 ttcagcttgt ggtcgacggc accgacccgg agctcgtcaa gggcattctc gatacggaga
      961 tcggcatcct ggaaacccgc cactcttcca acaaagcctt cttcgactca atggcggagc
     1021 tcgcgccggc tttcggtatg ctcgggacgc tcatcggcct tatcacgatg ctcggtaatt
     1081 tgcaggatcc caacgcgctc ggtccgggaa tggcggtcgc tctcgttact acgttctacg
     1141 gctcgctgat cgccaacggc tttgccattc cgatcgcaaa gaagctcgcc tcccgttcat
     1201 cacaggaagt cctctcgatg gagctgatgg tggagggggt tctttcgatt caggcgggag
     1261 agaacccgcg tatcgtcgag gagaaactca aggtcttcct tcctccgtcc cttcgtctgg
     1321 cgttggagga gaaggcaaag gcggcgaagg aagggggagc gtagatggcc cgcagcaaga
     1381 aaaaaaagga ggactcttct cccggagcgc cgctttggct tgtcacctac ggagacatgg
     1441 tcacgctggt gctcaccttt ttcgtgctgc tcttctcgat gtcgtcgatc gacgtgcaaa
     1501 agtttgagct gatgctcctc tccttcaagg gcgctatagg attcatgccc ggaggcaagg
     1561 cgatacagga ggattcggcc gtattcggag ggcagcccgc gcaacacgcc ggcgagtccg
     1621 ccagagtgac gcctgagatt cagcagatag cgcgcaacct tcggaactac cttcgcacag
     1681 aagggctcga taaagagatt gtggtccgta tcgaccagcg gg
//
LOCUS       DAOSWW010000649         1721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_60023_length_1721_cov_3.361345, whole genome shotgun sequence.
ACCESSION   DAOSWW010000649 DAOSWW010000000
VERSION     DAOSWW010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60023_length_1721_cov_3.361345"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PK442_11565"
     CDS             complement(<1..614)
                     /locus_tag="PK442_11565"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HOO88330.1"
                     /translation="MILSFRWYGESDPVTLDKIRQVPVLKGIVSALYDVPLGETWPLE
                     KIESLKAAVERKGFVLSAIESIPVHENIKLGRPDRDVLIDKYCASIRNMGKAGIPVLC
                     YNFMPVFDWTRSNMAFPLPDGSNALSYDDVEVLKMDLSKGTKDLPGWSTSYDAKELQA
                     LLDGYKELSEEGLWENLKYFLEKTVPVAEESNVKMAIHPDDPPWS"
     gene            complement(657..1412)
                     /locus_tag="PK442_11570"
     CDS             complement(657..1412)
                     /locus_tag="PK442_11570"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOO88331.1"
                     /translation="MTSKFSLEGKRALVTGGGSGIGLAISQGIVENGGSVVIVGRREE
                     LLKEACDSMGPKASYMVHDVTDLPGNVGFVKKLEAGGPVDILVNNAGQNIKKPVLETT
                     DEIYHNILEVNLVAVFSLTREISRFMLERKRGAIINIASMASLFGIPYVSAYTSSKAA
                     IAGLTKQLATELSPFGITVNAVAPGFIDTAMSRKAFEGDPERKRKVLSRTPMNELGLP
                     EDVANAVVFLASPAARYLTGVVLPVDGGTSIGF"
     gene            complement(1473..>1721)
                     /locus_tag="PK442_11575"
     CDS             complement(1473..>1721)
                     /locus_tag="PK442_11575"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88332.1"
                     /translation="VALRVGIDPIHFAMIAVLNLMIGLTTPPVGVCLFVASSIGKVSI
                     SRIARAGLPFLAVSIFVLLLVSYIPALSTWLPNLLFNR"
BASE COUNT          335 a          522 c          507 g          357 t
ORIGIN      
        1 gaccagggcg gatcgtcggg gtggatggcc atcttgacgt tcgactcctc cgctacggga
       61 acggtctttt caaggaagta cttcaggttc tcccagagcc cctcctcgct gagctccttg
      121 tatccgtcca gaagcgcctg aagctccttg gcgtcgtacg aggtgctcca gcccgggaga
      181 tccttcgtcc ccttcgagag atccatcttg agcacttcga cgtcgtcgta cgaaagggcg
      241 ttggagccgt ccggcaatgg aaacgccatg ttcgacctcg tccagtcgaa gaccggcatg
      301 aagttgtagc acagcaccgg aatacccgcc ttccccatgt tgcggatgga ggcgcagtac
      361 ttgtcgataa ggacatctct gtcgggacgc cccagcttga tattctcgtg caccgggatg
      421 ctctcgatcg cggagaggac gaaccccttc cgctcaaccg ccgctttcag gctctcaatc
      481 ttctccagag gccacgtctc gcccaaggga acgtcgtaga gcgcactgac gatcccttta
      541 agcaccggaa cctgtcggat cttgtcgaga gtgacggggt cgctctcgcc gtaccatctg
      601 aatgacagaa tcatgcgcaa tcctcctctc ttttgctctc cgcagtaagc aacagcttag
      661 aatccgatgc tcgtgccgcc gtccacggga aggacgacgc cggtaaggta gcgcgccgcg
      721 ggagatgcga ggaagacgac ggcgttcgcc acgtcctcgg ggagccccag ctcgttcatc
      781 ggggtgcggg agaggacctt gcgctttctc tcggggtcgc cctcgaaggc cttgcgcgac
      841 atcgcggtat cgatgaaacc gggcgccacg gcgttgacgg tgataccgaa gggggagagt
      901 tccgtagcaa gctgtttcgt cagccctgct atagccgctt tcgaggacgt gtacgcgctc
      961 acgtacggaa tgccgaagag cgacgccatc gaggcgatat tgatgatcgc gccgcgcttt
     1021 cgctcgagca tgaagcggct tatctcgcgt gtgagcgaga agacggcgac caggttgacc
     1081 tcgaggatgt tatggtagat ctcgtccgtg gtctccaaga cgggcttctt gatgttctgt
     1141 ccggcgttgt tcacgaggat gtcgacaggc ccgcccgctt ccagcttctt gacgaacccg
     1201 acgttgcccg gaagatccgt gacgtcgtgg accatgtacg acgctttcgg ccccatggag
     1261 tcgcaagctt ccttgaggag ttcctccctc cggcctacga tgaccacgga accgccgttc
     1321 tccacgatcc cctgcgagat ggcgagaccg atgccgcttc cgccgcccgt taccagggcc
     1381 cgttttccct caagcgaaaa tttggatgtc atgtcgtatt tctcctttca ctttctcgta
     1441 catacgccgc ttattctcct ggcggtagtg cttcagcggt tgaagagcag attgggcagc
     1501 cacgtcgaaa gcgctgggat gtaggagacc agcagcagga cgaagatgct cacagcaaga
     1561 aatggcagcc ctgcccgcgc gatacggctt atggagacct tcccgatgct ggaagcgacg
     1621 aagagacaga cgcccaccgg aggcgtggtc agcccgatca tcaggttgag cacggcgatc
     1681 attgcaaagt gaatcgggtc gatccccaca cgcagcgcta c
//
LOCUS       DAOSWW010000650         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_60193_length_1718_cov_3.280818, whole genome shotgun sequence.
ACCESSION   DAOSWW010000650 DAOSWW010000000
VERSION     DAOSWW010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60193_length_1718_cov_3.280818"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..>1718)
                     /locus_tag="PK442_11580"
     CDS             complement(161..>1718)
                     /locus_tag="PK442_11580"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOO88333.1"
                     /translation="AAENLTGEIRDAVKIHEKKPRGDAISAARNKLLEHFQAQYPESG
                     DYVAGLIDEMVKKTMRSLIVDERTRADGRRMDELRKITCETGVLPRVHGSALFTRGET
                     QALVVTTLGMMGVDDQILDGLKQDEPAKRFILHYNFPPFSVGEVRPMRGPGRREIGHG
                     ALAERALRSMIPDEADFPYVMRVVSDILESNGSSSQASICGGSLALMSAGVPMKKHVA
                     GIAMGLIKEGEKVAVLTDIQGLEDHFGDMDFKVAGTRDGVTALQMDNKAGGITRAILE
                     QALSQARAARMQILDSMEAAIPVPAELSANAPRIFMTTIDPEKIRDVIGPGGKVIRGI
                     TQKTGVKINVEDSGEIYIGGSSQEKVDEALSIIRGLTKDLEAGEVYHGTVTRLMTFGV
                     FIECLPGKEGLLHVSEVSTHRIPKVEDVFKVGDKVLVMVKEIDDMGRTNLTRRRILEN
                     ESRIAEEGLSEVLPAEQERETVIAALAEANKNNPQPERERPRDGGGRGDRRPGGGGSG
                     PRRHSSGGRS"
BASE COUNT          270 a          562 c          513 g          373 t
ORIGIN      
        1 ggatgccggt gccgacgctc gtccgctccc ccggcaggag aagcatgtcc tccgccgagc
       61 gaagatctgc gccggcagcg ctttccgttg cgtattcagg aagagggaac tcttgcgctc
      121 ttccttccct gagaattttt acagccaccg gcattggagg ctacgacctt cctcccgatg
      181 agtgcctgcg gggaccgctt ccgccgccgc ccggcctgcg gtcgcctctt ccgccgccgt
      241 cccgggggcg ttcccgctcc ggctgaggat tgttcttgtt cgcctccgca agcgcggcaa
      301 tcacggtctc tcgctcctgc tcggcgggca gaacctccga gagaccttct tcggcgatac
      361 ggctctcgtt ttcgaggatg cgtctgcgcg tcaggttggt gcgccccatg tcgtctatct
      421 ccttcaccat gacgagcacc ttgtcgccta ccttgaagac atcctccacc ttcgggatgc
      481 ggtgggtgct gacctcgctc acatgaagca gcccttcctt gcccggaagg cactcgatga
      541 agacgccgaa ggtcatgaga cgggtcacgg tgccgtggta gacctctcct gcctcaaggt
      601 ccttcgtgag gccgcggatg atggagagcg cctcgtcgac cttttcctga gaggagccgc
      661 cgatgtagat ttcgccgctg tcctcgacgt tgatcttgac gcccgtcttc tgcgtaatgc
      721 cgcggataac cttgccgccg gggccgatga cgtcgcggat cttttcgggg tcgatagtgg
      781 tcatgaagat tctgggcgcg ttcgcggaaa gctccgcggg aacggggatc gcggcctcca
      841 tgctgtcgag gatctgcatc ctcgccgcac gggcctggga gagcgcctgc tcaagaatcg
      901 ccctggtgat cccgccggcc ttgttgtcca tctggagagc ggtcacgccg tcgcgggttc
      961 cggcaacctt gaagtccatg tcgccgaagt gatcttcaag cccctgaata tcggtaagca
     1021 ccgcaacctt ctcgccttcc ttgatgagac ccatcgcgat tccagcgacg tgcttcttca
     1081 tcggaacgcc cgcgctcatc agagcgaggc tgccgccgca gatggacgcc tgggagctgg
     1141 agccgttgga ctcgagaata tccgagacca cgcgcatcac gtaggggaag tcggcttcgt
     1201 ccggtatcat ggagcgaagc gccctctccg cgagcgcgcc gtgccctatc tcgcgccgtc
     1261 ccggtcctct catgggacga acctctccga cggagaacgg ggggaagtta tagtgcagaa
     1321 tgaaccgctt ggcgggctcg tcctgtttca gtccgtcgag tatctggtcg tccacaccca
     1381 tcatgccgag cgtcgtcaca acgagcgcct gcgtctcgcc gcgggtaaag agcgcggagc
     1441 cgtgcactct cggaagaacg cccgtctcgc aggtgatctt cctgagctcg tccatccggc
     1501 gaccgtcagc ccgggttctt tcgtcgacga tgagcgaacg catcgtcttc ttcaccattt
     1561 cgtcgatgag tcctgcgaca tagtcgccgc tctcggggta ctgcgcctga aaatgttcca
     1621 gcagcttgtt tctggccgcg gaaatcgcgt cgccgcgggg ttttttctcg tgaattttca
     1681 ccgcgtcgcg aatctctccg gtaaggttct ccgccgcc
//
LOCUS       DAOSWW010000651         1715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_60387_length_1715_cov_2.724096, whole genome shotgun sequence.
ACCESSION   DAOSWW010000651 DAOSWW010000000
VERSION     DAOSWW010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60387_length_1715_cov_2.724096"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..709)
                     /locus_tag="PK442_11585"
     CDS             complement(152..709)
                     /locus_tag="PK442_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOO88334.1"
                     /translation="MALLRLRRDSAHFLMIDVQERLLPAISGHEAIHRNILKMERAAA
                     VMNIPFCFTEQYPKGLGGTAAEILASLPEGALRFEKSTFSCCDEEGFNDIVDRQGRPV
                     TVLFGIETHICVLATVLDLLELNRQVIVAADACGSRLHEHAVLAMDAMRGAGALVLPV
                     ETIIYHMMGRSGTPEFKALLPLFKE"
     gene            967..1608
                     /locus_tag="PK442_11590"
     CDS             967..1608
                     /locus_tag="PK442_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88335.1"
                     /translation="MTRERKNDGNEQNEIWTVGEILSAMSGSEYPQYIVKIVNQVQQW
                     MDESIRADNPEQDIDPVAALFENVMEMDGEALFNSLEWVDEYADLLPNIKKPFGNEDV
                     VALHIGPADFEDSLRMAIDYAALFNRGVSRRVWLISDTFIIGDVYRYISHIRALNRQG
                     IAFRFLLVTPWGWTEIPLAADSAPSSRIAWKNNRKGGTAPGEDDRKRDDRLTR"
BASE COUNT          404 a          436 c          452 g          423 t
ORIGIN      
        1 cattctctca agagcgcctg cgcgggcctt tcgattcctc agtgctgtgg cgaagcgagc
       61 gtcactcttt ttatgtaacg ctcgccggaa cgcttctatt gctcttcttc gtccgctgac
      121 agcgaacggg tcgtattttt gccccgagcc tctactcctt gaagagaggc agcagcgcct
      181 tgaactccgg cgtgcctgaa cggcccatca tatggtagat gatggtttcg accggaagaa
      241 cgagcgctcc cgccccgcgc attgcgtcca ttgcaaggac tgcgtgttca tgcaaacggc
      301 ttccgcaggc atccgcagct acgataactt gccgattcag ctccagcaga tcgaggactg
      361 tcgccaggac gcagatatgc gtttcaatac caaaaagaac cgtgactggc ctgccttgcc
      421 ggtcgactat gtcgttgaac ccctcttcat cacagcatga aaaagtgctt ttctcgaatc
      481 gcagagcgcc ttcgggcaga gacgccagga tttcagccgc ggtcccccca agtcccttcg
      541 gatattgttc tgtaaagcaa aaagggatgt tcataactgc tgctgcacgt tccatcttca
      601 gtatgttgcg atggattgct tcgtgacccg aaatagcggg gagcagccgc tcctgtacgt
      661 caatcataag aaagtgcgcc gaatcacgtc gaagacgaag aagagccatg atttcacctc
      721 ctgagaattt tcccctacga ttttacacta aaaagcatgc ggcggactgt cattgctttc
      781 tttttttctc ttcatccaac tcttttcagc agtcaatttc tttgacgaat ccggaatgaa
      841 ggatcttttt ttgaaaaaca gacgaacgcg aaactctttt ttctcacttc ttttgtttat
      901 tcttcctgtt gtgagcttct ggagtatgtg ctatgcttcc atcagatcag aaacacggag
      961 gatggcatga cacgagaacg aaagaacgac ggaaacgagc aaaacgaaat ctggacagtg
     1021 ggagagattc tttccgccat gtcgggatct gaatatccgc agtatattgt aaagatagtc
     1081 aaccaggtgc agcagtggat ggacgagtcg atcagagccg ataatcccga gcaggatatc
     1141 gacccggtcg ccgccctttt tgagaatgtg atggaaatgg acggagaggc tctctttaac
     1201 agtcttgaat gggtcgacga atatgccgat ctccttccga atatcaagaa acctttcgga
     1261 aacgaggatg ttgtcgcgct tcatatcggt ccggcggatt tcgaggacag tcttcgtatg
     1321 gccatagact acgccgctct tttcaatcgc ggggtgagcc gcagagtgtg gctgatcagc
     1381 gatacgttca tcatcggcga tgtataccgt tatatatctc atatacgcgc tttgaatcgt
     1441 caggggattg catttcgctt tctcctggtc actccctggg gatggaccga aataccgctt
     1501 gcggcggaca gcgcgccttc aagccgtatt gcatggaaga acaaccgaaa gggaggaacg
     1561 gctccgggag aagatgacag aaaacgagat gaccgtttga cgagatagcc ttcgggtgtt
     1621 acaatcttcg ccgttgccgg gaacgttcgg gaacacggtg tcggggcgta gcgcagtctg
     1681 gtaagcgcgc ctggttcggg accaggaggt cggag
//
LOCUS       DAOSWW010000652         1710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_60691_length_1710_cov_2.300302, whole genome shotgun sequence.
ACCESSION   DAOSWW010000652 DAOSWW010000000
VERSION     DAOSWW010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60691_length_1710_cov_2.300302"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PK442_11595"
     CDS             complement(<1..978)
                     /locus_tag="PK442_11595"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOO88336.1"
                     /translation="MDAIRRMIKDRCMEKEEPFCASACPFHLDVREFIARMQRGAFNT
                     AFRLFSNTTGFPAIVAAHCHEPCAAVCPRGTVDAPVQLNLLEKAAVAYAANTKPNSYN
                     LPPKKGRIAVVGAGLSGLGCALRLANRKYRVDVYERSGRVGGRLWDEMDPEIFLADIR
                     LQFSNEEYGLFLDHTVETLEPLRAQYDAVYVATGSGGDAFGLTCENDVREGIPWASRL
                     PGVFLGGALLGASSMEALSQGLKASLCIEDYLKTGAMRSAERVPPMRMRRPPEAIVRT
                     PVVLPADGALFSKEEAVAEASRCIRCRCDACVRHCGLMRSYKKFPGRIAE"
     gene            complement(1050..>1710)
                     /locus_tag="PK442_11600"
     CDS             complement(1050..>1710)
                     /locus_tag="PK442_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO88337.1"
                     /translation="GAYSNTSVPGLCRLDPNTGIGDPTPAFTYCLNLAEVEVDTSTGK
                     TSVLRFTCVDDVGKIGNIDAVNGQAYGGISHAIGFALGENYDDVKKHTNIAASGVPYI
                     ETIPDEIEVIHYERPDEAGPFGSSGASEGFQSSGHMAVLNAINNACGVRVYEMPATPE
                     KVKAGLALLASGGTIEPPKKYFLGSDLYEELENIKANPVQVGPEDYFVPLGGASAERF
                     F"
BASE COUNT          303 a          534 c          535 g          338 t
ORIGIN      
        1 ctcagcgatg cgtcccggaa acttcttgta cgagcgcatc aggccgcagt gacggacgca
       61 ggcgtcgcac cggcagcgaa tacagcggga ggcctccgcg acagcctctt ctttcgaaaa
      121 gagcgcgccg tcggcgggaa gcacgacggg cgtgcggacg atcgcttccg gcggccgccg
      181 catcctcatc ggggggactc tttccgcgct ccgcatcgcg cccgtcttca gatagtcctc
      241 gatgcagagc gaggccttca gtccttggct gagcgcttcc atggaactcg ctcctaagag
      301 cgcgccgccg aggaagactc cgggaagacg actcgcccat ggtatgcctt cgcgcacgtc
      361 gttttcgcag gtcaggccga aagcgtcgcc cccgctcccc gtcgcgacat agactgcgtc
      421 gtactgtgca cgcagcggct ccagcgtttc gaccgtgtgg tcgaggaaga ggccgtactc
      481 ttcgttcgag aactggaggc gtatatcggc gaggaagatc tccggatcca tctcgtccca
      541 aaggcgtccg ccgacgcggc cgcttcgctc gtagacgtcc acacggtatt tcctgttcgc
      601 cagccgtagc gcgcacccca gaccgctcag tcccgccccg accacggcga tccgcccctt
      661 cttcgggggg aggttgtagc tgttcggctt ggtattcgcc gcatacgcga cggccgcttt
      721 ttcgaggaga ttgagctgta cgggagcgtc gaccgttcct ctcggacaga ccgcggcgca
      781 cggctcgtgg cagtgcgccg cgacgatcgc gggaaagccg gttgtgttcg agaagagacg
      841 gaatgcggtg ttgaaagcgc cccgctgcat ccgcgcgatg aattcgcgca cgtcaagatg
      901 gaacggacag gccgaggcgc agaaaggctc ctccttctcc atgcagcgat cctttatcat
      961 gcgtctgatt gcatccatcg tccaatcccc tccggagatg ccgggagccc gtatcttcag
     1021 cagatacagg ctcccatgaa catgttttct tagaagaagc gttccgcgga ggcgccgccc
     1081 agaggaacga agtagtcctc gggcccgacc tggaccgggt tggccttgat gttctccagc
     1141 tcctcgtaga gatccgagcc gaggaagtac ttcttcggcg gctcgatggt cccgcccgaa
     1201 gccagcagag caagcccggc cttgactttt tcgggagtgg cgggcatttc gtagacgcgg
     1261 acgccgcacg cattattgat cgcgttgagc accgccatgt gcccggagga ctggaagccc
     1321 tcggatgcgc ccgaagaacc gaagggaccg gcttcgtccg gacgctcgta gtggatgact
     1381 tcgatctcgt ccggaatggt ctctatgtag gggacgccgc tcgcggcaat gttggtgtgt
     1441 tttttaacgt cgtcgtagtt ttcgccgagc gcgaagccga ttgcgtgcga gatgccgccg
     1501 tatgcctgtc cgttgacggc gtcgatgttt cctattttcc ccacgtcgtc gacgcacgta
     1561 aagcgcagca cgctcgtctt cccggtggaa gtgtcgacct cgacttcggc caagttaagg
     1621 caataggtaa aagcgggcgt cggatcgccg attcccgtgt tcgggtcgag tctgcacaaa
     1681 cccgggaccg aagtgttcga gtaggcgccg
//
LOCUS       DAOSWW010000653         1709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_60725_length_1709_cov_3.434099, whole genome shotgun sequence.
ACCESSION   DAOSWW010000653 DAOSWW010000000
VERSION     DAOSWW010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60725_length_1709_cov_3.434099"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1070)
                     /locus_tag="PK442_11605"
     CDS             complement(273..1070)
                     /locus_tag="PK442_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOO88338.1"
                     /translation="MKLFQVDAFTDRLFAGNPAGVCLLEAEVPDAWMQSMAAEMNLSE
                     TAFLLGTPSGWSLRWFTPRVEVALCGHATLSTAHIMLQEGMLQQGHEAVFHTKSGPLS
                     ARAEDEWIVLDFPAKKVVPVAPPPGLIEALGLERDEYLFCGKNKDDYLLELPDEELVR
                     SLDPDFVVLSDVEARGTMVTAPSSRCEFDFASRFFAPAVGVNEDPVTGSAHCALAPYW
                     GKKLGKTLMNAEQVSARGGVMLVEVQGERVLLRGKAVTVFRGEMTVS"
     gene            complement(1092..>1709)
                     /locus_tag="PK442_11610"
     CDS             complement(1092..>1709)
                     /locus_tag="PK442_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOO88339.1"
                     /translation="SSAAVIGCGGLGGLVADLLARAGVGRLVLVDGDVFDETNLNRQI
                     LSFEGNLGLSKSKVAAQRVTEINGVVRAEGRQCMFDEFTADGILEGCDLAVDCLDNLS
                     TRRILFSECRTRGIPVVSGAIGGFWGQIGVAMPDEQNLAEFLSGDTDKGVETETGNPP
                     FTPAVIAALECAQAVKILTGKGKLLTDQLLWMDLAEDEFTRLRLS"
BASE COUNT          368 a          560 c          456 g          325 t
ORIGIN      
        1 tatagtccat aacaacgtcg tagtaggcat cctgcatctt ccgtatactc accgctgcga
       61 aagcttcgtt gccctccccg cggccgcaac cgccaagcag aaacatcgaa caaagaataa
      121 aacaaacagg aacaaagacg atttttttca tcaggctccc ccttttttaa aacaccctta
      181 ccggcccgtt ttcagatatc cgctcgcttc gttataaaca tatactctac ccgacgcacc
      241 tcctttacct gttgctccga aaaaaagtgg gttcaggaaa ccgtcatctc tccgcggaag
      301 accgttacgg cctttcctcg cagaagcacc cgctcccctt gcacctcgac aagcatcacg
      361 cctccgcgcg cggagacctg ctcggcgttc atcagcgtct tccccagctt tttcccccag
      421 tagggggcaa gcgcacagtg cgcggagccc gtcaccgggt cttcgttcac gccaacagca
      481 ggcgcgaaga agcgggatgc gaaatcgaac tcgcaacgcg acgacggcgc cgtcaccatg
      541 gttcccctgg cctccacatc ggagagcacg acgaagtcgg gatcgagcga gcgtacaagc
      601 tcctcgtcgg ggagctcaag gagatagtcg tccttgttct tcccgcagaa gaggtattcg
      661 tcgcgctcca gcccgagcgc ctcgatgagc ccgggaggag gggcgacggg aacgaccttc
      721 ttcgccggaa agtcgagcac gatccattca tcctcggctc gtgcgctcaa agggccgctc
      781 ttcgtgtgaa agacagcctc atgcccctgc tgaagcattc cctcctggag cataatatgc
      841 gccgtggaga gcgtcgcgtg gccgcacaaa gccacctcga cgcgcggcgt aaaccagcgg
      901 agggaccacc cggatggggt tcccagaagg aacgccgtct cggagaggtt catctccgcg
      961 gccatgctct gcatccaggc atcaggaacc tcagcttcga gcagacagac gcccgccgga
     1021 tttcctgcga agagacgatc ggtgaacgca tcgacttgaa aaagtttcat gccgtttccc
     1081 cctttcgttt ttcagctcag gcgaagccgc gtgaactcgt cctcggcgag atccatccac
     1141 aacagctggt ccgtgaggag ttttcccttg ccagtgagga tcttcacggc ctgagcgcat
     1201 tcaagcgcgg cgatcacagc aggagtaaag ggagggttgc cggtttcggt ctcgaccccc
     1261 ttgtccgtat cgcccgacag gaattccgca aggttctgtt cgtcaggcat ggcgacgccg
     1321 atttgccccc agaagccgcc gatggccccc gagactaccg ggattcccct ggtccggcac
     1381 tcggagaaga gaatgcgccg cgtgctcagg ttatccaggc agtccacggc cagatcgcac
     1441 ccttcgagta tgccgtccgc ggtgaattcg tcgaacatgc actgccgacc ctcggcgcgg
     1501 acgactccgt tgatctccgt aacgcgctgc gccgcaacct ttgatttgga gagtccaagg
     1561 ttcccctcga aggagagtat ttgacggttc agattcgtct cgtcgaaaac atcgccgtcg
     1621 acaagcacca gacgtcccac accggccctc gcaagaagat cggcgacaag cccgccgagc
     1681 cctccgcagc ctataaccgc cgctgaaga
//
LOCUS       DAOSWW010000654         1706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_60916_length_1706_cov_3.078134, whole genome shotgun sequence.
ACCESSION   DAOSWW010000654 DAOSWW010000000
VERSION     DAOSWW010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60916_length_1706_cov_3.078134"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK442_11615"
     CDS             <1..186
                     /locus_tag="PK442_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOO88340.1"
                     /translation="VRVETGDLLFGDMGGVVVVPHAVEGEVIEKALEKAEGENLVAKA
                     IMGGMSTVEAFATFGIM"
     gene            218..1231
                     /locus_tag="PK442_11620"
     CDS             218..1231
                     /locus_tag="PK442_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HOO88341.1"
                     /translation="MRSAKLVAPRQIEIFEDRMPLPGEGEVLVRVKAAGICGSDLHIY
                     QGERADVTLPRILGHELVGEVESSGGGADRFSKGDRVTIDPVVSCGSCVSCKRGYDNL
                     CSTVKCLGVQVDGGYCDFIVVPEEKVYALPSSLPWEKAALIEPFSIAAEVLSRSEVRE
                     GDKVLVLGAGTIGLCILQAMKLAGAEVLTSDFVESRLEKAKELGADRVVNSKTGDLAA
                     ALREFTGEFGADVVVEAVGIPRLLEESLGYAAPGGRIVVLGFNPEPAKIPEVLVVKKE
                     LKIIGSRMNCRKFPQVIEWFEKGLVNPEPLISAKYPFEKINDAFKDILRDPQKYLKVL
                     ITY"
     gene            1277..>1706
                     /locus_tag="PK442_11625"
     CDS             1277..>1706
                     /locus_tag="PK442_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO88342.1"
                     /translation="MILDLFKLDGKTAIVTGARKGLGYGIAEGLAEAGANIVGVGPIA
                     MPEIETRVKALGRKFLHVEADLTSQARIPDIVAETVSAMGGVDILVNNAGIIRRADIL
                     EFTEKDWDDVMNVNLKSLFFLSQEAARQMAKQGRGGKIVNI"
BASE COUNT          337 a          424 c          622 g          323 t
ORIGIN      
        1 gtccgcgtgg agacgggaga ccttctcttc ggcgatatgg gcggcgtcgt ggtagttccg
       61 cacgccgtgg agggcgaagt catcgagaag gcgctggaga aggccgaggg agaaaacttg
      121 gtggcgaagg cgatcatggg aggcatgtcc accgtggagg cctttgcgac ctttgggata
      181 atgtagattc tggcccgaaa gggcggaggt gaatgatgtg agaagcgcaa agctcgttgc
      241 acccaggcag attgaaattt ttgaggacag gatgcccctt cccggcgagg gagaggtgct
      301 cgtccgtgtg aaggctgccg ggatctgcgg ttccgacctg catatctacc agggggagag
      361 ggcggacgtg acgctccccc ggattcttgg ccacgagctg gtgggcgagg tagagtcctc
      421 cgggggcggc gcggaccgct tctccaaggg ggacagggtg acgatcgacc ctgtggtttc
      481 gtgcggaagc tgcgtttcgt gcaaacgggg gtacgacaac ctgtgcagca cggtcaagtg
      541 tttgggggtg caggtcgacg gcgggtattg cgatttcatc gtcgtcccgg aggagaaggt
      601 gtacgcgctc ccgtcctctc tgccgtggga gaaggcggcg ctgatagagc ccttctcgat
      661 cgcggcggag gtgctttcac gaagcgaagt ccgtgaaggg gacaaggtgc tcgtccttgg
      721 agccgggaca atcggcctgt gcattctgca ggcgatgaag ctcgccggag ccgaggtgtt
      781 gacgagcgat ttcgtggaga gccggctgga gaaggcgaag gagctaggcg ccgacagggt
      841 tgtgaacagc aagaccggcg accttgcggc ggcgctgcgg gagttcaccg gagagttcgg
      901 cgccgatgtg gtggtcgagg ccgtgggcat accccggctt ctggaggaga gcctaggcta
      961 cgccgcgcct ggagggcgta tcgtggtgct cggcttcaac ccggagcctg cgaagatccc
     1021 cgaggtgctc gttgtgaaga aagagctgaa gatcatcggg tcgcgcatga actgccggaa
     1081 gtttcctcag gtgatcgagt ggttcgagaa ggggttggtg aacccggagc cgcttatctc
     1141 cgcgaagtac cccttcgaga agatcaacga cgcgttcaag gatattctgc gtgatccgca
     1201 gaagtacctt aaggtgctta tcacctactg ataagccggt tactcatttt ttccagagag
     1261 gaaggcgaat gaacagatga ttctggatct ttttaagctg gacggtaaga ccgccattgt
     1321 caccggagcg cgcaaggggt tgggctacgg catcgcggag gggcttgccg aggccggggc
     1381 gaacatcgtc ggcgtgggac cgatcgcgat gcccgagatc gagacgaggg tgaaggctct
     1441 cggacggaag ttcctccacg tggaggcgga cctgacctct caggcacgca tccccgacat
     1501 cgtggcggag accgtgagtg cgatgggagg cgtggatatt ctggtgaaca acgcgggcat
     1561 catccgccgc gccgacattt tggagttcac cgagaaggac tgggacgacg tgatgaacgt
     1621 caacctgaag agccttttct tcctgtcgca ggaggccgcc cgtcagatgg cgaagcaggg
     1681 ccgggggggc aagattgtga acatcg
//
LOCUS       DAOSWW010000655         1705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_60982_length_1705_cov_3.223030, whole genome shotgun sequence.
ACCESSION   DAOSWW010000655 DAOSWW010000000
VERSION     DAOSWW010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60982_length_1705_cov_3.223030"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PK442_11630"
     CDS             complement(<1..421)
                     /locus_tag="PK442_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOO88343.1"
                     /translation="MEGKQRATGGVQSIERAFAIIELLDAQGELGIAEMSRLLSLERS
                     TVHRIVSTIKNLGYINQNPLNHKYSNSFKFFEIGNDVVKRLGLRQQAAPFLRELSEKT
                     HEAVNLAILDGDRVIYIDKIESQSTIKVDLSVGKRFPA"
     gene            complement(778..1416)
                     /locus_tag="PK442_11635"
     CDS             complement(778..1416)
                     /locus_tag="PK442_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOO88344.1"
                     /translation="MALLRDMTLGGFTDELAADSAAPGGGSVAALSGALGAALVSMVC
                     RLTIGKKGYEEYELEVKDALAESEVLRKRLLEAIDIDAKAFEKVMEAFAAPKSTDEEK
                     AARREMIQTAFKGACESPRRTAADCLEVLRLCARVADKTNTNAASDLGVGASNALAAL
                     ESAAMNVLINLPSIKDDEYVDELRDEMEAIQEEGGILRTGVLREVSAHIGGN"
BASE COUNT          374 a          489 c          430 g          412 t
ORIGIN      
        1 acgcagggaa gcgcttcccc acagaaagat ccaccttgat ggtagattgg ctttctatct
       61 tgtcgatgta gatcaccctg tcgccgtcga gtatggcgag attcaccgcc tcgtgcgtct
      121 tctcggaaag ttcccggaga aagggagcgg cctgctggcg aagaccgagc cgtttgacaa
      181 catcgttgcc gatctcgaag aatttaaagc tgttcgaata cttgtgattg agggggttct
      241 gattgatgta gccgagattt ttgatggtgg acacaatacg atggaccgta ctgcgctcca
      301 aggaaagcag acggctcatt tctgcaattc caagctctcc ctgagcgtcc agaagctcga
      361 taatcgcgaa agcgcgttct atggattgga ctccgcccgt ggccctctgc ttcccttcca
      421 tactttttcc tccataccat ccgagagaat ctcgcgtttc atttcccgca ttacggaatg
      481 atattgatta tagtggaatt gatcttgcgg atcattataa taaccggttc tttctcatgt
      541 caatagaaaa agagcgcaat tccgccaaga cagtaagttc tttttttatc aattatgacg
      601 cggcaaaagg gagaagagcg ttgcgacgga tactacgaaa gggaaaaaga cagatccggt
      661 ttggaaaata tgggacaggg gcgacacgca acaacaaccg ccgttttgac gttcgaaagg
      721 aaaaggaccg cgtcagcaca aagcgcggtc ctttaaaacc cgacgaatcc gaaacgatta
      781 gttcccgccg atatgcgccg atacttcgcg caagactccg gtccgcagga taccgccttc
      841 ctcctggatg gcctccattt cgtcgcgcaa ctcatcgacg tactcatcgt ccttaatcga
      901 aggaaggtta atcaagacgt tcatagcggc gctttcgagg gcggcaagcg cattggacgc
      961 tcccactccg agatcgcttg ctgcgttcgt attcgtcttg tcggcgaccc gagcgcacag
     1021 acgaagcact tcaaggcaat ccgcagccgt ccggcgcggc gactcgcagg cgcccttgaa
     1081 agcggtctgt atcatctccc ggcgcgcagc cttctcctcg tcggtactct tgggcgcggc
     1141 aaatgcctcc ataacctttt cgaacgcctt cgcgtcgata tcgatcgctt ccagaagacg
     1201 cttccggaga acctcggatt cggcgagcgc atccttcacc tcgagctcgt actcttcata
     1261 cccctttttg cctatggtaa ggcggcagac catcgagacc agagcggccc cgagagctcc
     1321 tgagagcgcg gcgacgctgc cgcctccggg tgcggcggaa tcggcggcca gttcgtcggt
     1381 aaatccacca agggtcatat ctcttagaag tgccacagtg cttcctcctt tgcgcagtgc
     1441 attttttccg agagcggttc gttcatcctc caactctcgc cgtatcgaca cttttacaat
     1501 gttctcaagc aacgctctat ttatatcaca tttcacgcgc cccggggcct ttttctcgta
     1561 tctcttcatc gaaagagcgg aaagagcgct ttgcgctctt ccgccctctt gtccggccga
     1621 ttgctttccc gttacgggaa cctgtttgct tagaagagcc ccgtgatgcg gcccttttcg
     1681 tcgatgtcta tcgacagcgc cgcag
//
LOCUS       DAOSWW010000656         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_61079_length_1704_cov_1.696180, whole genome shotgun sequence.
ACCESSION   DAOSWW010000656 DAOSWW010000000
VERSION     DAOSWW010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61079_length_1704_cov_1.696180"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK442_11640"
     CDS             <1..388
                     /locus_tag="PK442_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88345.1"
                     /translation="TVSLPADARIAQIRRIPDAKLHRECKQLPRKLIALLYKEGWTEK
                     SVDEKRLLSPLWAPCLSKEEVESLFTLEPLASIRKEMWCSVSFWEDVSLLESVEKLAD
                     DVGEVFFEAGEWTLCPFELNDQHDEG"
     gene            911..1063
                     /locus_tag="PK442_11645"
     CDS             911..1063
                     /locus_tag="PK442_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88346.1"
                     /translation="MAGGGMVLSGGSALAMIAAGTLIWSSLEGERQSQTLVKRYLLLI
                     VKNVFN"
     gene            1102..>1704
                     /locus_tag="PK442_11650"
     CDS             1102..>1704
                     /locus_tag="PK442_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88347.1"
                     /translation="MKKCLMLVLIFIYSFFFKVDGTAAWTDVYFNAEVSLSHYVNEDA
                     HFLGIWNADKYRTGCDDFDNAPMKLSQDVALILVAYLIASETNETYGSVYQGMADTRL
                     DVYRKNVKNPFEKSAITNAHNKLVEYYRNALKSGKKINLPEYYYIKRVDSNFFDVNDD
                     RNVYYFDAFNLHMSKFAVRSLRIHMSDDSGVRKRIPWRITS"
BASE COUNT          438 a          352 c          435 g          479 t
ORIGIN      
        1 tacggtgtcg ctcccggctg acgcccggat cgcgcagatt cgccgaatcc ccgatgcaaa
       61 gcttcatcgg gaatgcaaac agctccctcg aaaactgatc gcgctgcttt acaaggaagg
      121 atggactgag aagagtgtcg atgaaaaaag gctgctctcc cctctctggg ctccctgcct
      181 ttcaaaagag gaagtcgaat cgctttttac gcttgaaccc ctcgcgagca ttcgaaagga
      241 gatgtggtgt tccgtttcgt tttgggaaga tgtcagcctt ctcgagtccg tcgaaaagct
      301 cgccgacgat gtcggggagg tctttttcga ggccggcgaa tggactctgt gcccgttcga
      361 actgaacgac cagcacgatg aagggtgatt cggagcagac tgtatttcga ttcggaacgc
      421 accgtgccgc ttcgcgagtt gttctggata acgcacgaag cattccatat cggcgagtgc
      481 tctcgaaatg taacgaactc ccggctcttt tcaacggagg cctgcaatgc cgcacgcatg
      541 ccgtgaacag aatgaacaaa aaggaaatca atgaacaaat ccctttgcga gcttgtataa
      601 ataagctata atcagttttg tgttttgcct ctatattcag tcgtttttaa ggttgctgga
      661 gtacagctac gtactccata ttaatatcat ccaagatgct gctcatgtcc ggggatctct
      721 cgttttttcg ccttcgaaac gtccgtcgag aagtgtgcgc gaatgatgga gaagatcgaa
      781 gccgctgttc ttttcggaag cgcgggtacc ggcgccgcca tatcctctct ttctggattt
      841 ccgcgactaa cgctgtaatg ctgtcttcgc gtggcttgga ggaggtaccg ctgcagatgg
      901 aggctggagt atggccggtg gcgggatggt tctttccggt ggttccgcgt tggcgatgat
      961 tgcagcggga acactcatat ggagttcttt agagggcgag cggcagagcc aaactcttgt
     1021 gaagagatat ctattgctta tagtgaagaa tgtatttaat tgaggtgtga aaaaaagaca
     1081 aatcacagga gggtattgaa aatgaaaaag tgccttatgt tagttttaat atttatttat
     1141 tcgttttttt tcaaggtcga tggaactgct gcttggacag atgtttattt taatgcagaa
     1201 gtcagtttgt ctcactatgt aaatgaagat gcccactttt tgggtatctg gaacgcggat
     1261 aaatacagaa ccggttgcga tgactttgat aatgcaccga tgaagttgtc ccaggatgtg
     1321 gcactgattc tcgtggctta cttgatcgct tccgaaacaa acgaaacgta tggaagtgtt
     1381 tatcagggga tggcagatac gcgtctggat gtgtaccgga aaaatgtaaa gaaccctttt
     1441 gaaaaaagcg ccattaccaa tgcgcataat aagcttgttg aatactatcg taatgcctta
     1501 aaaagtggca aaaagattaa tctaccagaa tattactata ttaaaagagt tgattcaaat
     1561 ttttttgacg taaacgatga tagaaatgtt tattattttg atgcgtttaa tcttcatatg
     1621 agtaagttcg ctgtccgttc gcttcgtatt catatgtccg atgattcagg agtcaggaag
     1681 cgtattcctt ggcgcattac tagt
//
LOCUS       DAOSWW010000657         1703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_61114_length_1703_cov_2.722087, whole genome shotgun sequence.
ACCESSION   DAOSWW010000657 DAOSWW010000000
VERSION     DAOSWW010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61114_length_1703_cov_2.722087"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..428)
                     /locus_tag="PK442_11655"
     CDS             complement(111..428)
                     /locus_tag="PK442_11655"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO88348.1"
                     /translation="MSFPVEFLPEAADDYKKLDGSVRKDVNKKIDELAENPFLGERLG
                     HKFSIDLTGFLKIYVHGKKYRIIYRLIEPERIEIIEIWGIGKREKEKMYKLIGERVKK
                     RTK"
     gene            complement(428..700)
                     /locus_tag="PK442_11660"
     CDS             complement(428..700)
                     /locus_tag="PK442_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOO88349.1"
                     /translation="MLVETRRIMPITRLQRELTQTVRDISETGEPVYIVKNNTMEAVM
                     VPFEEYEYLAALEEMFEYYEIDDVVQKRLGAYDPGKNIRWSELRED"
     gene            783..911
                     /locus_tag="PK442_11665"
     CDS             783..911
                     /locus_tag="PK442_11665"
                     /inference="COORDINATES: protein motif:HMM:NF018476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HOO88350.1"
                     /translation="MRRHKQDKRMVERIDRLIREIQRDPFAGIGKPEALKHAPAGY"
     gene            complement(942..1439)
                     /locus_tag="PK442_11670"
     CDS             complement(942..1439)
                     /locus_tag="PK442_11670"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOO88351.1"
                     /translation="MLTVIKADITTLHVDAIVNAANNSLLGGGGVDGAIHRAAGPELL
                     EECRKLGGCDTGDAKLTKGYRLPAKYVIHTVGPVWHGGKSGEPGLLASCYRRSLEIAQ
                     SAGAKSIAFPSISTGVYGYPADQAAKIAVDTVRETLKTCPGIEKVVFCCFSEKDRAVY
                     EGVIG"
     gene            complement(1445..>1703)
                     /locus_tag="PK442_11675"
     CDS             complement(1445..>1703)
                     /locus_tag="PK442_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88352.1"
                     /translation="ADAWSMCLGFMIHMIPSAVLAVVLVISWRFELFGAAAYFLLALF
                     YYVLTKGKEDLLAYISISGPMLLISVLFFLNWLLIKRRKGE"
BASE COUNT          386 a          479 c          410 g          428 t
ORIGIN      
        1 tttcaaagcc cctcatccga ttactctcct ctttgcatgc gcagggctat ccggtctgta
       61 cgaattcggg ccgaaaatca tggcggcgaa caactccttg acggcgggcc ctacttcgta
      121 cgcttcttta ctctttctcc gatcaattta tacatcttct ctttttccct ttttccaatt
      181 ccccaaattt ctattatttc aatcctctcc ggctcaatca ggcgatagat aattctatat
      241 ttcttgccat ggacatatat cttcaggaag cccgtcaagt cgatcgaaaa cttatggccc
      301 aaccgctctc ctaaaaacgg gttttcggcc aactcgtcga tttttttatt cacatcttta
      361 cgtacgcttc catccaattt cttataatca tccgccgcct caggtaaaaa ctcgaccggg
      421 aacgacatca gtcttctctt aattccgacc aacgtatatt tttgccagga tcataagcgc
      481 ccaaacgctt ttggacgaca tcgtctatct catagtactc gaacatctcc tcaagagccg
      541 ccaaatactc atactcctca aaaggcacca ttacggcctc catggtgttg ttctttacga
      601 tgtataccgg ttcgccggtt tcggaaatat ctctgacggt ttgagtcagt tctctttgca
      661 gacgagttat cggcattata cggcgcgttt ccaccaacat gtcgaccacc tccaatgttg
      721 attataatac atactttagc atgtagtcaa atacatgcta tttacgcccg tgagaagact
      781 gtttgcgccg gcataaacag gacaagcgga tggtcgagcg gatcgacagg ctgatcaggg
      841 agattcagcg ggatccgttc gcgggaatag gaaagcccga agccctgaag catgctccgg
      901 ccggttattg agtccgatcc gtcgcattac ttcaaaaccc gttacccgat caccccctcg
      961 tacaccgccc ggtccttctc ggagaagcag cagaagacga ctttctcgat ccccgggcag
     1021 gttttgagcg tctcgcgtac tgtatccacg gcgatcttcg cggcttggtc cgcggggtag
     1081 ccgtagacgc cggtgctgat actcgggaag gcgatcgact tggctcccgc gctttgggct
     1141 atttccaggg agcggcggta gcaggaggcg aggaggccgg gttcgccgct ctttcctccg
     1201 tgccagacgg gacctacagt gtggatgacg tactttgccg gaaggcggta ccctttggtg
     1261 agtttggcgt cgccggtgtc gcagccgccc agctttcggc actcttcgag cagttcgggg
     1321 cccgccgcgc ggtggatggc cccgtccact ccgccgccgc ccagcaggga gttgttggcg
     1381 gcgttgacga tggcgtccac atgcagcgtg gtgatgtccg ctttgattac ggtcagcatg
     1441 gcgctcactc tccttttcgc ctcttgatca gaagccaatt gagaaaaaag agcacgctta
     1501 tgaggagcat tggccccgaa atggaaatgt atgcgagcag gtcttctttc cctttggtca
     1561 agacgtaata gaacaaagca agaaggaaat atgccgctgc gccgaacagt tcaaaacgcc
     1621 atgagataac aagaacaact gcgagaaccg cggaggggat catatgaatc ataaatccca
     1681 aacacatcga ccatgcatcg gca
//
LOCUS       DAOSWW010000658         1699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_61331_length_1699_cov_3.288929, whole genome shotgun sequence.
ACCESSION   DAOSWW010000658 DAOSWW010000000
VERSION     DAOSWW010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61331_length_1699_cov_3.288929"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK442_11680"
     CDS             <1..382
                     /locus_tag="PK442_11680"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOO88353.1"
                     /translation="GCQVEIGAAGAMAAAAVVEAAGGSARQGCDAAATVFQNMMGSVC
                     DLVQGVVEIPCHSRNAALASQAFLCADLVLGGYRSPVPLDETIDAVDSTGRMLPEELR
                     CTSRGGLALCPSAQRLPRLDKKIS"
     gene            395..1165
                     /locus_tag="PK442_11685"
     CDS             395..1165
                     /locus_tag="PK442_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOO88354.1"
                     /translation="MTTVLSSIKEGVCRIELNRPEAMNTFNEDLAIGLDEAFRAADAN
                     DEVRVIIVSGAGKHFSTGIDLRELLSKERHEIPGFLKLMDLHNHRLASLTKPVIASVH
                     GYALANGTGLALACDFVVASEDAVFGTTAVNVGLICLEPGYQLARWIGEKRALQYVLS
                     GELIPASKGLELGFVHTMTARETLEEKTLELAKKLASKSALSLRAGKVGFARMEGLAL
                     ERAIQAGGDIFAALAASEDGREGVEAFLAKRPPLFKGR"
     gene            complement(1186..>1699)
                     /locus_tag="PK442_11690"
     CDS             complement(1186..>1699)
                     /locus_tag="PK442_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOO88355.1"
                     /translation="RTRQRVLEGRQLALLFENQRLGPADSIGLTVHVDMFLNMIDHDA
                     FLFFPYIEKKLTVLHITRGRGDRLRIEREDSLFGAIQKVLKLDAVRVIKVGGEESEAV
                     AFSEQRAGSGGNTFNLEPGRVCIWDRNVATIKALEKGGIKVVPLDADELVKGGGGPRC
                     STMPLWRDDV"
BASE COUNT          375 a          498 c          499 g          327 t
ORIGIN      
        1 gggatgccag gtagagatag gcgccgcggg agcgatggcc gcagccgcag ttgtagaggc
       61 cgccggagga agcgcgcgcc aggggtgcga cgcggccgcg acagtatttc aaaacatgat
      121 gggatccgtc tgcgacctgg tgcagggagt ggttgaaata ccctgccaca gcagaaacgc
      181 cgcgctggca tcgcaggcgt ttctctgcgc ggatcttgtg ctcggcgggt accgaagccc
      241 ggtgcctctg gacgaaacga tcgacgccgt cgacagcaca ggaaggatgc tccccgaaga
      301 gttgcgttgc acatcaagag gcggtctcgc tctctgtccg tcggcgcagc gccttccacg
      361 gctcgacaaa aaaataagct gaagggagaa aaaaatgaca acagtgctca gcagcataaa
      421 agaaggcgtt tgccgtatcg aactgaacag gccggaagcg atgaacacat tcaacgaaga
      481 ccttgcaatc ggtcttgacg aggcgttccg tgcggccgac gcgaatgacg aggttcgcgt
      541 catcatcgtt tccggagcag gtaagcactt ttcgacggga atcgacctcc gggagttgct
      601 ctcgaaagag cggcacgaaa tccccggctt cttgaagctc atggatctgc acaaccacag
      661 gctcgcatcg ttgaccaagc cggttatagc ttccgttcat ggatatgcgc tcgccaacgg
      721 aacgggtctc gcacttgcct gtgattttgt tgtcgcatcc gaggatgcgg tcttcggaac
      781 aacagcggtc aacgtcgggc tcatctgcct cgagccaggg tatcagcttg cccgttggat
      841 aggagagaaa cgcgcgctac agtacgtcct ctccggcgaa ctcatccccg catcgaaggg
      901 gctcgaactc gggttcgtcc atacaatgac ggcaagagaa acgcttgagg agaagacgct
      961 ggaactggca aaaaaacttg cgtcgaagag cgcgctttcg ctccgtgcgg gaaaggtagg
     1021 attcgctcgc atggaggggc ttgctctcga gcgcgcgata caggccggcg gagatatctt
     1081 tgccgcactc gccgcatccg aggacgggag ggaaggtgtc gaagccttcc tcgccaagcg
     1141 ccctcccctc ttcaaaggcc gctgacatcc ctgtggaggg acggcttaca cgtcgtccct
     1201 ccacagcggc atcgtcgagc atcggggacc tccgccgcct ttcacaagtt cgtccgcgtc
     1261 aagcggaacg accttgatcc cgcctttttc aagcgccttg atggtcgcga cgtttctgtc
     1321 ccagatgcac acacgccccg gttcgaggtt aaacgtattt ccgcccgaac ctgcgcgctg
     1381 ctcggaaaag gcgactgcct cgctctcctc gccgccaacc ttgataacgc ggactgcgtc
     1441 cagcttgagc actttttgaa tggccccgaa cagagaatcc tccctctcaa tccgcaggcg
     1501 atcccctctt cccctcgtga tatgaagaac agtgagtttt ttttctatat aggggaagaa
     1561 gagaaacgcg tcatggtcga tcatgttcaa aaacatatcg acatgcacgg tcaggccaat
     1621 agagtccgca ggtccgagac gctgattttc gaagagcaac gccagctgcc gcccttcgag
     1681 cacgcgctgg cgagtgcgc
//
LOCUS       DAOSWW010000659         1697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_61443_length_1697_cov_4.375761, whole genome shotgun sequence.
ACCESSION   DAOSWW010000659 DAOSWW010000000
VERSION     DAOSWW010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61443_length_1697_cov_4.375761"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1563
                     /gene="ltrA"
                     /locus_tag="PK442_11695"
     CDS             22..1563
                     /gene="ltrA"
                     /locus_tag="PK442_11695"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HOO88356.1"
                     /translation="MTGDVNTSFVLDMQRKLYRWSSEDPNKVFADLFNLVCDRRTLNE
                     AWRRLARNSGSQTPGTDGMTRRSVEQRPGGAAGLLDDIRGRLRDGTYRPEPVRQRLIP
                     KPGKPGEFRPLGIPTLTDRLVQMALKLILEPVFEADFYPTSYGFRRGRNAHDALERIQ
                     KCLHPTKRGPSVYRYVIEGDIKGCFDAIDHHVMMERVRRRISDRKVLKLILAFLKAGV
                     MVEGTVRHSVTGSPQGGIISPMLSNIYLTAIDERYGRWSMRPREPSINAAARRITDHR
                     RGRPVFYMVRYADDFVVLVEGAREQAEAEKRALAEFLKKELRMELSMEKTKITDVREG
                     FDFLGYRVVQEKAARPNKHGRRPQVGKLFIPKSKLNDLRYTIKSLVKSIPTGWSLANV
                     IDRLNPILLGWRNYYRYATGAYREFSRLDFWIWQRVGRWLKMKHGNASWPVLRRRYHL
                     SAPGKRRQWTEKPKRLRHLTEGRTMRYPNQSIEKPNGWNAKWKTRQRDSMYDFQDSFR
                     RLKHI"
BASE COUNT          401 a          473 c          513 g          310 t
ORIGIN      
        1 aagcaagcag gaggaagggt catgaccggc gatgtgaata cgtcgttcgt cctcgacatg
       61 caacgcaagc tgtatcggtg gagcagcgaa gaccctaaca aggtgttcgc tgatctgttc
      121 aatctcgttt gcgaccgcag aaccctgaac gaagcctggc gacggctcgc ccgcaatagc
      181 ggcagccaaa caccgggaac ggacgggatg acgcggcgtt cggtcgaaca gcgccccggc
      241 ggcgcagcgg gactcctcga tgacattcga ggacgtctcc gcgacggaac ttatcgcccg
      301 gaacctgtca ggcaacggct tatcccgaag cccggcaagc ccggagagtt ccggccgctc
      361 ggcattccga cgctgaccga ccgtctggtg caaatggctt tgaagctcat cttggagccc
      421 gtcttcgagg cggattttta tccgacctcg tacggattcc gaagaggcag aaacgctcat
      481 gatgctctgg agagaatcca gaaatgcctt cacccgacaa agcgcggacc ttcggtctac
      541 cgctacgtca tcgaaggcga catcaagggg tgtttcgacg ccatcgatca tcatgtcatg
      601 atggagcgcg tgcgacggcg catcagcgac cgcaaggtgc tgaagctgat cctcgctttc
      661 ctgaaagccg gcgtgatggt cgaagggacc gttcgtcatt ccgtcaccgg ctcgccgcaa
      721 ggcgggatca tctcgccgat gctgtcgaat atctatttga cagccatcga cgagcggtac
      781 gggcgatggt ccatgcgacc tcgcgaacca agtataaacg ccgccgctcg acggatcacc
      841 gatcaccgac gaggcaggcc ggtgttttac atggttcggt atgcggacga ctttgtcgtt
      901 ctcgtcgaag gcgcgaggga gcaagcggaa gctgaaaagc gcgcgctggc ggagttcctg
      961 aagaaggaac ttcgcatgga gttgtcgatg gaaaagacga agatcaccga tgtcagggag
     1021 ggcttcgact tccttggcta tcgggtggtg caggaaaagg cggctcgccc aaacaaacac
     1081 gggcgacgcc cccaagtcgg caaactcttc attccgaaga gcaagctgaa cgacctgcgt
     1141 tacacgatca agagtctggt gaagagcatc cctacgggat ggtcgctcgc caacgtcatc
     1201 gatagactta atccaatcct gttgggatgg aggaactatt accgctacgc cactggcgct
     1261 tatcgggagt tcagccgtct cgacttttgg atatggcagc gcgtcgggcg ctggctgaag
     1321 atgaagcacg gaaacgcatc gtggccagtg ctgcgacgac gctatcatct cagcgctccg
     1381 ggtaaacggc ggcaatggac tgaaaagccc aaacgcctgc gtcacctcac tgaaggaagg
     1441 acgatgcgtt acccgaacca gagcatagag aaaccgaacg gatggaacgc gaagtggaag
     1501 accagacagc gtgacagcat gtatgacttc caggactcgt tccgacgcct caaacatatt
     1561 tgaggaccac ccatgggcgc gcaaacgccc atgcagggag agccggatgc ttggaaacct
     1621 gcacgtccgg ttcggagagg gggatggaga aacttgtctc cgtaaggtga taaagcgctt
     1681 catctctact ctacggg
//
LOCUS       DAOSWW010000660         1695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_61588_length_1695_cov_2.484756, whole genome shotgun sequence.
ACCESSION   DAOSWW010000660 DAOSWW010000000
VERSION     DAOSWW010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61588_length_1695_cov_2.484756"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PK442_11700"
     CDS             complement(<1..346)
                     /locus_tag="PK442_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88357.1"
                     /translation="MGKKGILFLVWFLGFVSAVEGAATWYNAYTGGMWNNPGSALIDT
                     MLYNRMMAETSLKKMQRTPSGGVGASGQVRTLYRPSSSQSSIPAKVAAVLSNDNATKK
                     ELTVFFKEGLQSF"
     gene            689..1279
                     /locus_tag="PK442_11705"
     CDS             689..1279
                     /locus_tag="PK442_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOO88358.1"
                     /translation="MTIKRCPNGHFYDPDANNSCPWCAVPADDNERTRRINPAAIPVA
                     GNAGSTVKLSPRNTSSSGSSIDEGHTVAVVKRRTGIDPVVGWLVCVKGPDRGRDYRIR
                     SEKNSVGRADSMDICISGDDTISRNDHAFVVYDPRKKIFRIQAGLSRGLVYLNGEEVI
                     ASELLAAYDTISVGQSELLFIPLCGERFQWETEQES"
     gene            1255..>1695
                     /locus_tag="PK442_11710"
     CDS             1255..>1695
                     /locus_tag="PK442_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO88359.1"
                     /translation="MGNRTGILNRILSAVCLLLLLAGAALAAPSTLRVRQAYLDYPSL
                     TVYADLLDAAGKPFVPGGAEQLTATVGGRAFDVSRLSAFSAKNDGVLIIFLVDTSASI
                     GQNRFTELQETVSGWIRRMGPSDRAAVMSFCENVSVVQGFTAERH"
BASE COUNT          380 a          496 c          423 g          396 t
ORIGIN      
        1 caaaactctg caacccttct ttgaaaaata cagtcagttc cttctttgta gcgttatcgt
       61 tgctcaaaac agcggcaacc ttggcgggta tggaggactg agaggaagaa ggccggtaga
      121 gcgtccgcac ctgccccgaa gctcccactc caccggaagg cgtcctttgc atcttcttca
      181 acgaggtttc tgccatcatt ctgttataga gcatcgtgtc aattaacgcg gagccggggt
      241 tgttccacat tccccccgta taggcgttgt accacgtagc ggctccttca accgcggaaa
      301 caaaacccaa aaaccatacg agaaacagaa ttcctttttt ccccattccg tatcgccccc
      361 ttcgaaaaag agtcgctgcc cgtacgaggt ccctcctttt tacgcatatt ctactcgttg
      421 cagtttcaca tgagcaagag ttcttttcat aaaggtgaaa agggcttttt gccttgattg
      481 gatgccgaag aagctgttga aaaaagtgtt tttttggcgt atggtactct tgtttataat
      541 atacgcctcc gaatgccctt tcgcattttt ttaggtatcc agaatacatt tttgacgcta
      601 ccctttccga atgagcggaa aaagtggtga attgtcgatt tctgcttctg tgggatatca
      661 tctcaattct gaaacggagt gattcgaaat gacgatcaaa cgatgcccga acggacactt
      721 ctacgatccc gacgccaata attcttgtcc gtggtgcgct gttcccgccg acgacaacga
      781 aagaacgcgg cgaatcaacc ccgcagcgat accggtggcg ggcaacgcag ggtccacggt
      841 gaaactctct ccccgaaaca catcctcgtc cggaagctcc atcgacgaag ggcataccgt
      901 cgccgtcgtc aagcgcagga cgggaatcga tccggtcgtc ggctggcttg tctgcgtcaa
      961 gggtccggac cgcggacgtg actaccgtat acgctcggag aagaactcag tcggcagggc
     1021 cgactccatg gacatctgca tctccggaga cgatacaatc tcgcggaacg accatgcatt
     1081 cgtcgtctac gaccccagaa agaagatctt cagaatccag gcgggattga gcagaggtct
     1141 ggtctatctc aatggagaag aggtcattgc ctcggagctg ctcgccgcct atgacacaat
     1201 ctcggtcggg cagagcgaac ttcttttcat tcctttgtgc ggggagcgct tccaatggga
     1261 aaccgaacag gaatcctgaa ccgcatcctc tccgcagtct gcttgcttct tttgcttgca
     1321 ggcgccgcgc tcgccgcacc ctcgacattg agagtccgcc aggcgtacct cgactatcct
     1381 tctctaaccg tgtatgccga ccttctcgac gccgccggaa aaccttttgt ccccggcgga
     1441 gccgagcaac ttaccgcgac cgtaggcgga cgcgccttcg atgtgagccg cttgagcgct
     1501 ttttccgcga agaacgacgg agtccttatc atcttcctcg tcgatacctc cgcctcaata
     1561 ggacagaacc gcttcaccga actgcaggag acggtctccg gatggatccg ccgtatgggc
     1621 ccctccgacc gcgcggccgt gatgtcgttc tgcgagaacg tctccgtcgt ccaaggcttc
     1681 accgccgaac gacat
//
LOCUS       DAOSWW010000661         1694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_61637_length_1694_cov_2.942648, whole genome shotgun sequence.
ACCESSION   DAOSWW010000661 DAOSWW010000000
VERSION     DAOSWW010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61637_length_1694_cov_2.942648"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PK442_11715"
     CDS             <1..795
                     /locus_tag="PK442_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOO88360.1"
                     /translation="GRENAPLSGKRIAVVKELQNRKDAVSAEVEDVLQKTLALCREEG
                     AEIVEISLPVSLEFGLPCYYILAPAEASSNLARFDGVRYGLSSDEKSLVNLYFKTRGS
                     GFGSEVKRRILTGTYVLSSGYYDAYYLTAQKVRQLMKREFAAAFAGCDLIALPTAPSP
                     AFRKGELVDDPIQMYLADVFTLPVNLAGLPALSLNGGFTSGEKPLPVGVQFIAPKWGE
                     PELLSTAAVLEKKLGKAKTAPCDWAETSAERAANAKTAGSERGGAR"
     gene            792..>1694
                     /gene="gatB"
                     /locus_tag="PK442_11720"
     CDS             792..>1694
                     /gene="gatB"
                     /locus_tag="PK442_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOO88361.1"
                     /translation="MKYTYQPVIGLEIHVQLATATKIFCSCSTDYIGAEPNTNVCPVC
                     LGLPGALPVLNGKAVELGIKAAMALSCTVKDKTVFHRKNYFYPDLPKAYQISQYDLPL
                     AEGGELFISGDGDTLKRIRITRLHLEEDAGKLVHSAADGRLEGAEFSFVDYNRGGVPL
                     AEIVSEPDLRSPGEAREYVTSLRRLVRYLGVSDGDMERGSMRVDANISLQKTDAATGE
                     ILYWGERAEIKNMNSLRAIERALEYEIRRQIDVMEAGGVLERETRHWNDGEGTTTSMR
                     SKEAAHDYRYFPDPDLPPIEASSAL"
BASE COUNT          374 a          481 c          499 g          340 t
ORIGIN      
        1 ggacgggaaa acgctcctct ctccgggaag agaatcgccg tagtgaagga actgcaaaac
       61 aggaaagacg ccgtttccgc cgaggtggag gacgttctcc aaaaaacgct tgcactctgc
      121 cgggaggagg gggccgaaat tgttgaaata tcccttcccg tctcccttga attcggcctg
      181 ccgtgctact acatcctcgc tcctgccgaa gcgagttcca accttgcccg gttcgacggc
      241 gtccgttacg gcctctcttc ggacgaaaaa tccttggtga acctctactt taaaactagg
      301 ggctccggtt tcggcagcga agtgaaacgg aggattctca cgggaacgta tgttctcagc
      361 tcgggatact acgacgccta ctacctgacc gctcagaaag tcaggcaact tatgaaaagg
      421 gaattcgcgg cggcattcgc cgggtgtgat ctcatcgccc ttcccacggc tccttctccg
      481 gcatttcgaa agggagagct agtggacgac cccatccaga tgtatttagc ggatgtcttt
      541 actcttcccg tgaacctcgc cggactcccg gcgctctcgt tgaacggggg cttcacatcg
      601 ggtgaaaagc cgctccctgt aggggttcag ttcatcgctc ccaaatgggg ggagccggag
      661 ctcctttcga cggcggcggt gcttgagaag aagctcggca aggcaaaaac cgctccgtgc
      721 gattgggcgg aaacctccgc ggagagggcg gcaaacgcca aaaccgccgg ttcggaaagg
      781 gggggcgcca gatgaaatat acctaccaac cggtcatcgg tcttgagatt cacgtccagc
      841 ttgcgacagc aacgaaaatt ttctgctcct gttccacgga ctacatcggc gccgagccga
      901 atacgaacgt ttgccccgtc tgcctcggcc tgccgggagc gctccccgtg ctcaacggga
      961 aggctgtgga actcgggatc aaggcagcca tggcgctctc ctgtacagtc aaggacaaga
     1021 cggtctttca cagaaagaac tacttctacc ccgacttgcc gaaagcatat cagatcagtc
     1081 agtatgacct tcctctggcg gagggggggg agctcttcat ctccggggac ggcgatacgc
     1141 tgaagcgaat acggatcacc cgcctgcatc ttgaagagga tgccgggaaa ctggtgcatt
     1201 cggcggccga cggccgactc gaaggggctg agttctcttt tgtcgactat aacaggggcg
     1261 gcgttccgct ggctgagatt gtttcggagc ctgatcttcg ttcgccaggc gaggctcgcg
     1321 agtacgtgac ttcgcttcgc aggcttgttc gttatctcgg cgtctccgac ggcgacatgg
     1381 agcgcggttc catgcgcgtc gacgcgaata tttcgctgca gaagacagac gccgcgacag
     1441 gtgagattct ctactgggga gagcgcgccg agataaaaaa tatgaactcg cttcgcgcga
     1501 tagaacgcgc cctggaatac gagattcgca ggcaaatcga cgtcatggaa gcaggaggcg
     1561 ttctcgaacg ggaaacgagg cactggaacg acggagaagg aacgaccacc tcgatgcgaa
     1621 gcaaggaggc ggcccacgat tatcggtatt ttcctgatcc cgatctgcct ccgatcgaag
     1681 cgtcttccgc acta
//
LOCUS       DAOSWW010000662         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_61762_length_1692_cov_2.929750, whole genome shotgun sequence.
ACCESSION   DAOSWW010000662 DAOSWW010000000
VERSION     DAOSWW010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61762_length_1692_cov_2.929750"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK442_11725"
     CDS             complement(<1..203)
                     /locus_tag="PK442_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88362.1"
                     /translation="MSALRVENLNVHYGTIHAVQGVSFSLEEREIVSIVGSNGAGKST
                     VMWTLAGVVERSGGSVDIFGESLP"
     gene            complement(200..985)
                     /locus_tag="PK442_11730"
     CDS             complement(200..985)
                     /locus_tag="PK442_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO88363.1"
                     /translation="MSESKVLLSVAGLTKDFGGIRAVDSFSFEVREGSITGIIGPNGA
                     GKTTVFNLVTGLYKPTSGTIRFLGEEIGGNRPDVIVRHGIARTFQNIRLFNRRTCLEN
                     VLTPLLQRERCSFLGAMLGLPSVRRMERTLHDKAHSLLAALGLDTFASFEASTLPYGL
                     QRKLEIARALATEPSLLLLDEPAAGMNPEETHSLANLIAEVREKFNVTILLIEHHMDL
                     VMNICSPIVVMNFGALLATGTPEAVRANPEVVKAYLGERKEKR"
     gene            complement(982..>1692)
                     /locus_tag="PK442_11735"
     CDS             complement(982..>1692)
                     /locus_tag="PK442_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO88364.1"
                     /translation="YYYVDYYLALVAGAAAAGIVSLIIGVPTLRAKLRSDYFAIAVLG
                     FGEALRVLLENLEITNGARGLPGIDSFTTLPIVFACLAGGIFVARNFLRSRWGAACIA
                     IREDPIAAETAGVRLFRTRLLSLVVSAVYCGLSGGLLAHFLSFIQPQMFTLVQSTQLL
                     AAVIAGGMGSISGPLIASFVFIALPEALRVAQMWRLVAYGLLLVLIMVYRPQGLMGYS
                     EVASFFRRKSGPEGGRRS"
BASE COUNT          353 a          526 c          509 g          304 t
ORIGIN      
        1 gggagggatt caccgaagat gtccaccgaa ccgccggagc gttccaccac tccggcgagc
       61 gtccacatga cggtcgactt ccccgcgccg ttcgacccaa cgatcgagac gatctcccgc
      121 tcttcaaggg agaacgaaac tccctgcact gcatggatag ttccgtaatg gacgttcaga
      181 ttctcaaccc gcagagcgct catctttttt ctttcctttc acccagataa gccttgacga
      241 cttccgggtt cgcccgtacc gcttcgggcg ttcctgtcgc cagcagcgcg ccgaagttca
      301 tcaccacgat gggcgaacag atattcatta cgagatccat gtgatgctcg atgagaagaa
      361 tcgtcacatt gaacttctcg cgcacctccg cgataaggtt cgcgaggctg tgcgtctctt
      421 ccgggttcat accggccgcc ggttcgtcga gcaacagaag cgaaggctcc gtggcgagcg
      481 cgcgcgcgat ttcaagctta cgctgtagac cgtacgggag cgtgctcgcc tcgaaggaag
      541 cgaacgtgtc gagtcctaat gccgccagca gggaatgcgc cttgtcgtga agcgtgcgtt
      601 ccattcttcg gacggacggc agcccgagca tcgcgccgag gaagctgcac cgctccctct
      661 ggagcagcgg cgtgagtacg ttttcaaggc aggtccgccg gttgaaaaga cgtatattct
      721 ggaacgtcct tgcgatcccg tggcggacga taacatccgg gcggttgccg ccgatctcct
      781 ctcctaggaa acggatcgtt cccgaagtcg gtttgtacaa tcctgtgacg aggttgaaga
      841 ccgtcgtctt ccccgcgccg ttcggaccga tgatacctgt gatcgagccc tctcggacct
      901 cgaaagagaa ggagtccacg gcgcgtattc cgccaaagtc ctttgtcagg cccgcaacag
      961 agagcaatac tttcgattcg ctcatgagcg ccgcccccct tccgggccgg atttcctccg
     1021 gaagaatgaa gcgacctcgg agtaccccat caatccctgc ggacgataga ccatgatgag
     1081 cacaaggagc aggccgtatg caacaagacg ccacatctgc gccactctga gcgcctcggg
     1141 gagcgcgatg aacacgaagg aggcgatcag cggccccgag atgctcccca tgcctccggc
     1201 gatgaccgcc gcgagaagct gcgtcgactg caccagcgtg aacatctggg gctggatgaa
     1261 cgagagaaag tgcgcgagca accctcccga gagaccgcaa tagacggcgc tcacgacgag
     1321 ggagagcagg cgggttctga agagcctgac gcccgccgtc tcggctgcga tggggtcctc
     1381 gcggatggcg atgcacgccg ctccccagcg ggagcgaagg aagttgcggg cgacgaagat
     1441 cccgccggcg aggcaggcga agacgatggg gagcgtcgtg aagctgtcga tccccggaag
     1501 tccgcgtgcg ccgttcgtga tctcgaggtt ttccaaaagc acccggagcg cttcgccgaa
     1561 gccgagcaca gcgatggcga agtagtcgct ccgcagtttc gccctgagcg tcggcacacc
     1621 gatgatcagg ctgacgattc cggcggccgc cgctcccgcg acgagcgcca gataataatc
     1681 gacgtaatag ta
//
LOCUS       DAOSWW010000663         1690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_61887_length_1690_cov_4.097248, whole genome shotgun sequence.
ACCESSION   DAOSWW010000663 DAOSWW010000000
VERSION     DAOSWW010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61887_length_1690_cov_4.097248"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1115
                     /locus_tag="PK442_11740"
     CDS             135..1115
                     /locus_tag="PK442_11740"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO88365.1"
                     /translation="MSKKLFALALLVMFTVILTASAASAAVVLKLSNVLPDGNPTTES
                     LVFFAEKVKEKTGGEVEVRLFNNSALGGQREALESMQAGTLEMCMANAGPMAQFVPTM
                     DVLSLPYVFQSTEHMFKALNGKAGEMIKADIDKAGFVFLYWGDGGSRNLINNKKPITK
                     PEDLKGLKIRVMDSRLMVNTLNAMGAIATPMAQGEVYSALQQGVLDGWENNPVTLLTL
                     KLYEVSDYFSWTQHFMVPDLVLISKQAFEKLTPEHQKALLEAGRESEAKQREYWNAFV
                     GKTVEELKSHNVKFNDVDIAPFVQSVQPVWEDYTKKFGTELLDLITSAAK"
     gene            1191..>1690
                     /locus_tag="PK442_11745"
     CDS             1191..>1690
                     /locus_tag="PK442_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOO88366.1"
                     /translation="MARWTTDKELFSLMKNELFTALVGDVLDTMGLTKQFLPPRIRPL
                     LPETTLAGRAMTVLEADCCGAGRKPFGLMFEALDDLKEDEVYICAGGSPDFAHWGGLM
                     TIRAKHLNAAGAVTHGYTRDTRQVLELDFPVFSWGTYAQDQGARGRVIDWRCPVEFVN
                     GVRVETG"
BASE COUNT          352 a          454 c          576 g          308 t
ORIGIN      
        1 gacggacatc gaacgaaggg cggtggaaac gggcggctga gtatgcgaac agcagcgtga
       61 acaggaatcg cccctcggcg gcgaagaggg gaagaagagc agtctatcag cagatacgcg
      121 aaggaggttg cagtatgtcg aaaaagttgt ttgctcttgc gctccttgtg atgttcacgg
      181 ttattctgac tgcgtccgcg gcgtccgccg cagttgttct gaagctgtcc aacgttcttc
      241 ccgacggcaa tccgacgacg gagtccctgg ttttcttcgc cgagaaggtg aaggagaaga
      301 cgggcggcga ggtggaagtg cgcctcttca acaacagcgc gctcggcggt cagcgcgagg
      361 cgcttgaaag catgcaggcg ggcacgctcg agatgtgcat ggccaacgcc gggccgatgg
      421 cccagttcgt tcccacgatg gacgtcctct ccctccccta cgtcttccag agcacggagc
      481 atatgttcaa ggcgctcaac gggaaggccg gagagatgat caaggcggac atcgacaagg
      541 ccggattcgt cttcctctac tggggcgacg gcggttcgcg caacctgatc aacaacaaga
      601 agccaatcac gaagcccgaa gacttgaagg ggctgaagat ccgcgtcatg gacagccgcc
      661 tgatggtgaa cacgctcaac gcgatgggcg ccatcgccac gccgatggcg cagggcgaag
      721 tgtacagcgc gctgcagcag ggcgtgctcg acggatggga gaacaacccc gtcacgctgc
      781 ttacgctgaa gctctatgag gtgtccgact acttctcgtg gacgcagcac ttcatggtgc
      841 ccgaccttgt gctcatcagc aaacaggcgt tcgaaaaact gactcccgaa catcaaaaag
      901 cgctgttgga ggccgggcgc gagtccgagg ccaagcagcg ggagtactgg aacgcgttcg
      961 tcggcaagac ggtggaggag ctgaagtcgc acaacgtgaa gttcaacgat gtggatatcg
     1021 ctcccttcgt ccagtccgtg cagccggtgt gggaggacta tacgaagaag ttcggaacgg
     1081 agctgctcga ccttattacg tcggctgcga agtaacggga cgttcttttt tccggaggag
     1141 ggcggcgcgc cgcgcccctt cctccgttca acgaaaggcg ggaacctggc atggcgagat
     1201 ggacaacgga taaagagctg ttctctctga tgaaaaatga gctgtttaca gcgcttgtgg
     1261 gcgatgtgct ggacacgatg gggctgacga agcaatttct tccgccgcgg atacggccgc
     1321 ttctgccgga gacgacgctt gccggacggg cgatgacggt gctggaggcg gactgctgcg
     1381 gggcgggccg gaagcccttc gggttaatgt tcgaggccct cgacgatctg aaggaagacg
     1441 aggtgtacat ctgcgcgggt ggctcgccgg acttcgcgca ctggggcggt ctgatgacca
     1501 tccgggcgaa acatctgaat gcggccggag cggtgacgca cggctatacg cgcgatacgc
     1561 ggcaggtgct ggagcttgat tttccggtgt tctcgtgggg aacgtacgcg caggatcagg
     1621 gggcgcgcgg acgcgtgatc gactggcgct gtccggtcga gttcgtcaac ggcgttcgcg
     1681 tggagacggg
//
LOCUS       DAOSWW010000664         1689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_61984_length_1689_cov_2.434517, whole genome shotgun sequence.
ACCESSION   DAOSWW010000664 DAOSWW010000000
VERSION     DAOSWW010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61984_length_1689_cov_2.434517"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="PK442_11750"
     CDS             complement(<1..109)
                     /locus_tag="PK442_11750"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HOO88367.1"
                     /translation="MSRRTDIPAFYSPWLFRRLKDGYVLVRNPRNAKQTR"
     gene            complement(137..604)
                     /locus_tag="PK442_11755"
     CDS             complement(137..604)
                     /locus_tag="PK442_11755"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOO88368.1"
                     /translation="MLCVRKTSIGRIAIGGEEEYVTHLYLPRSTELIERFDAKETPVI
                     REAFRQLELYLEGKLREFSLPLRAEGTPFMKRVWSELVKVPYGATTSYKELAEASGNP
                     KAVRAVGTANAKNPIAIFIPCHRVINTGGKLGGYGGGLDLKKTLLELEARNRP"
     gene            948..>1689
                     /locus_tag="PK442_11760"
     CDS             948..>1689
                     /locus_tag="PK442_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOO88369.1"
                     /translation="MTQTHNGQTQRGYYGEYGGRFVPEGIAELLAVLERAFDDAVADG
                     AFQKELDTLLVEYVGRPSAITECVNLSEECGGGRLFLKREDLNHTGAHKINNALGQAL
                     LAKRMGKKRLIAETGAGMHGTASATAAALMGMECTVYMGAVDVERQAPNVSRMKALGA
                     KVVPVHDGQATLKEAVDSALMAWMNDLEAFYLLGYVVGPSPYPEVVRYFQSVIGREAR
                     TQMLEREGRLPDEVAACVGGGSNAIGLFS"
BASE COUNT          326 a          495 c          543 g          325 t
ORIGIN      
        1 agcgcgtctg cttcgcgttc cgcggattgc ggacgaggac atacccatcc ttcaaacggc
       61 ggaacagcca cggggagtag aatgccggga tgtcggtccg tctgctcacg ctgactatgc
      121 gcacacgaac gacatcctac ggtctgttgc gggcttccag ctcgaggagc gttttcttca
      181 ggtcgagccc cccgccgtat ccgccgagct tgccgccggt gttgatgacg cggtggcagg
      241 ggatgaagat ggcgatgggg ttcttcgcgt tcgccgttcc cacggcgcgg accgccttcg
      301 gattccctga ggcctcggcg agctccttgt agctcgtggt cgccccgtag ggaactttca
      361 ccagctcgct ccagaccctc ttcatgaagg gcgtcccctc ggcccggagg ggcagcgaga
      421 actccctgag tttcccttcg aggtacagct ccaactggcg aaaggcctcc cggatcacgg
      481 gcgtctcctt cgcatcgaag cgttcaatca actccgtact cctcggcaga tagagatgcg
      541 taacgtattc ctcttccccg ccgatggcga ttcttcctat cgatgtcttc cgaacgcaca
      601 gcattgtggt cctcctctcc aactgatctc tttccgcccg gctattttct ctcaaaaacg
      661 aaaaaaaagc gatttgaaaa atgcgcattg acaaatcagg gttcgccgtg ctattgtccc
      721 tgcatgaaaa agacagcgca cacactcgac cggagtttta cgacgatgaa catgtggtgg
      781 tggcggccct agggtcccgc cgcctttgac ctgttttcag gtggccgcgg gagcaaacgt
      841 ttcccgcggc cctttttgta tccgtagttt caaagggccg tcgcttgaga cggccctttt
      901 tgtattcagc gaagagcatc ttcgcaactt cgaaggagga gatcgaaatg acgcagacac
      961 acaacggaca gacacaacgg gggtattacg gggagtacgg cggacggttc gtgccggagg
     1021 ggatcgcgga gctgctggca gtgctggaga gggccttcga cgacgcggtg gcggacggcg
     1081 cgtttcaaaa agagctggac acgctgctcg tcgagtacgt aggcaggccg tcggcgatca
     1141 cggagtgtgt caacctctcg gaggagtgcg gcgggggacg ccttttcctg aagcgggagg
     1201 atctgaacca caccggcgcg cacaagatca acaacgcgct cggacaggcg ctgttggcga
     1261 agcgtatggg aaagaagcgt ctcatcgccg agacgggcgc gggaatgcac ggcacggcga
     1321 gcgcgacggc ggcggccctg atggggatgg agtgcacggt gtacatgggg gctgtcgacg
     1381 tcgagcgtca ggcgccgaac gtctccagga tgaaggcgct gggcgcgaaa gtcgtcccgg
     1441 tacacgacgg ccaggcgacg ctcaaggagg ccgtggacag cgcgctcatg gcgtggatga
     1501 acgacctgga ggctttctat ctcctcggct acgtcgtcgg accgtcgccg tatccggaag
     1561 tcgtgcgcta cttccagagc gtcatcggac gggaggctag gacacagatg ctcgaacggg
     1621 aaggccggct tcccgacgag gtggccgcat gcgtcggcgg cggaagcaac gccatcggcc
     1681 tcttctccg
//
LOCUS       DAOSWW010000665         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62017_length_1688_cov_3.697489, whole genome shotgun sequence.
ACCESSION   DAOSWW010000665 DAOSWW010000000
VERSION     DAOSWW010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62017_length_1688_cov_3.697489"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1029)
                     /locus_tag="PK442_11765"
     CDS             complement(97..1029)
                     /locus_tag="PK442_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOO88370.1"
                     /translation="MNNQNIRAVAVGAANIDVHGFPGQPLVMRDSNPGHIRTCPGGVA
                     RNIAENLARLGVPVALITALGDDAGGASLRASCRELGIRLDRSVEVPGHPSSTYMAIM
                     DEEGDMALGLSDMRILDHVTPEHLESCAPLLSEADVVAADACLTTETLERLVNGPCAK
                     VFIDPVSIGKCRRMERLLGRFYCVKMNRMEAAYLAGMTIETGGDLDETARRMLASGVK
                     KLYITLGAEGVFFAEEGNSGRVPAIPLLPVNATGAGDAFTAAAVYGELHGWTMERTAR
                     FAVKASALTLLSRDTVSEQMSAAAVEAVGVHPTC"
     gene            1239..>1688
                     /locus_tag="PK442_11770"
     CDS             1239..>1688
                     /locus_tag="PK442_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88371.1"
                     /translation="MDAQEDRTKSSTESGKDRHPHAGAGKISLKEALGGEATDVHLFP
                     AVSSTPEDAPLSGAKVRVRIGGVLGPLTAVDADVDGELLALLNNPPKPDFSTSLPSGR
                     RLRVAVRTVCSGAAQEIFIRVLPETIPPVFGKLAHLLPEIAGMAPGLV"
BASE COUNT          292 a          529 c          549 g          318 t
ORIGIN      
        1 ggccctccct ccggccaaag cttcccggac ctccggcgcg atgcggactc tttcctttaa
       61 gatgctgcgc tcgttcatga gaatcattct cctttattaa caggtagggt ggactccgac
      121 ggcttcgacg gcggcggcgc tcatctgctc gctcaccgtg tcgcggctca ggagcgtgag
      181 cgcggaggcc ttcaccgcga agcgggctgt ccgttccatc gtccatccgt gcagctcgcc
      241 gtagaccgcg gctgcggtaa acgcgtctcc ggcgccggtg gcgttcacgg gaagcagcgg
      301 gatggccgga acccttccgc tgtttccctc ttccgcgaag aaaacgcctt cggctccaag
      361 ggtaatatac agttttttca cccccgacgc cagcatgcgg cgggcggtct cgtcgaggtc
      421 gccgccggtt tcgatggtca ttccggctaa gtatgcggcc tccatcctgt tcatcttgac
      481 gcagtagaaa cgaccgagaa ggcgctccat cctccggcac ttgccgatgg agacggggtc
      541 gatgaagacc ttcgcgcacg gtccgttcac cagccgttca agagtctccg tggtcaggca
      601 ggcgtccgcg gcgacgacgt cggcttcgga cagaaggggg gcgcacgact ccagatgttc
      661 gggcgtcacg tggtcgagga tgcgcatgtc ggagaggcca agcgccatgt ccccctcctc
      721 gtccatgatc gccatatagg tcgaggaggg gtgtcccgga acctccacgc tgcggtcgag
      781 gcggatgccg agctcgcggc aggaggcgcg aaggctcgct ccgcccgcgt cgtccccgag
      841 cgccgtgatc agggcgacgg gaacgccgag gcgcgccagg ttctccgcga tgttccgcgc
      901 caccccgccg gggcaggtcc ggatgtgtcc gggattcgaa tcccgcatga ccagcggctg
      961 acctggaaag ccgtggacat cgatattggc tgcgccaacg gcgacggcgc ggatgttttg
     1021 gttgttcaca cgcttttcct ccctggctgt ccgtcgtttc ggaagcgcgg cactccgtct
     1081 cttccgtcac cgggcgttcc aaaaaacggg atgctgaatg aacgcatggt caggctctct
     1141 acgcctcgga ttgatgatac actattccgc agaaagcggt gaaaagttgg tactgctaat
     1201 ggaccgtata tgtcgtatac aaaacaaggt gaacgccgat ggatgcacag gaagatagaa
     1261 cgaagagcag taccgaaagc gggaaagatc gccatcctca tgcaggcgca gggaaaattt
     1321 cgctgaaaga ggcgctcggc ggggaggcga cggatgtcca tctcttccct gcggtgtctt
     1381 ctacgcccga ggacgctccg ctcagcggag cgaaggtgag ggtgcgcatc gggggcgtgc
     1441 tggggcccct gacggcggtg gacgcggatg tcgacggcga gctgctcgcg ctgctcaaca
     1501 acccgccgaa acccgacttc tcgaccagtc tgccgtccgg aaggcggctc cgcgtggcgg
     1561 tccgcacggt ctgttccggc gccgcgcagg agatcttcat ccgtgtgctg ccggagacga
     1621 tcccgccggt gttcggcaag cttgcccacc tgcttccgga gatcgccggg atggctcccg
     1681 gcctggtg
//
LOCUS       DAOSWW010000666         1688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62019_length_1688_cov_3.471525, whole genome shotgun sequence.
ACCESSION   DAOSWW010000666 DAOSWW010000000
VERSION     DAOSWW010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62019_length_1688_cov_3.471525"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PK442_11775"
     CDS             complement(<1..1521)
                     /locus_tag="PK442_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88372.1"
                     /translation="MRFFSVRRFCVFMIAWAVAGGFSGAASALENTWTYQRIDSLYIE
                     IPDSWSPYSKKGSSGFYTGKPPSGGASVGSLVLLAGREKRNRYEDVLAPLRRSPGGSI
                     EALFHEEEKVVGGKDAVLFTNEISRREKDEAARPFIRMRSVVWKDAAEDGVLLLAVGG
                     DAALFDEHAADIDRMIASVRFDEPPLRQPGRKLEGIGGHAAVGEPVLPPIDLPPIELE
                     SGETAENEETKEGPPAEKTSRERPVFNSDALLEEEIRKARTALSLRDEGELLLAKGDR
                     EGAMDRFRGSLANVPDEDLSAFVETLGRRASEEHKAEEGRASLLRREGMELQRDGKLH
                     EALTKYRESLAVNDDETVREIVLGLEKLLQERASASVAQGEAHEAGGRLEEARAAYAE
                     SLSRVDEEFVRRRIAAVERLIRERQEAASPERVLAATLRNEGKALEEEGRLYEALGKY
                     RESLKSYEEQELLSRADALETELKERARARIREGGALQRAGKHAEALEKFRESRRYY"
BASE COUNT          285 a          603 c          465 g          335 t
ORIGIN      
        1 gtagtacctc cggctttcac ggaacttttc gagggcctcc gcatgcttcc cggcacgttg
       61 cagcgctccg ccctcgcgga tacgcgcgcg ggcccgctct ttcaactccg tctccaacgc
      121 gtcggcgcgc gaaagcagct cttgttcttc gtatgatttc aggctttcgc ggtacttccc
      181 gagcgcctcg tagaggcgtc cctcttcttc cagcgccttg ccttcattcc gaagcgttgc
      241 ggcgaggact ctctccgggg acgcggcctc ttgccgctcc cggatcaggc gctccaccgc
      301 ggcgattctg cgccggacga actcctcgtc gacccgggaa agactctccg cgtacgcggc
      361 gcgcgcctcc tcaagccgcc ctccggcctc gtgcgcctcc ccctgcgcga cagaggccga
      421 agccctctcc tgcaggagtt tttcgagtcc gaggacgatc tccctcaccg tttcgtcgtc
      481 gttcacggcg aggctctcac ggtacttcgt cagcgcctcg tgcagtttcc catcccgctg
      541 caactccatc ccctcgcggc gcaagagaga agcgcggcct tcctcggcct tgtgctcttc
      601 cgacgcgcga cgccccaaag tctccacgaa ggcggacaga tcctcgtccg gcacgttcgc
      661 gagacttccg cggaaacggt ccatcgcgcc ctcccggtcg cccttcgcca gcaggagctc
      721 tccttcgtcc ctgagcgaca gcgccgttcg cgccttccga atctcctctt caagaagcgc
      781 gtccgaattg aagaccgggc gctctcgcga cgtcttctcc gccggcggac cttccttcgt
      841 ttcttcgttc tccgctgttt ctccgctctc cagttcgata ggcggcaggt cgatgggcgg
      901 cagaaccggc tccccgacgg cggcgtgtcc tccgattccc tcaagcttcc tgccgggctg
      961 ccgcaggggc ggctcgtcga agcgcacgga agcgatcatc cgatcaatgt ccgcagcatg
     1021 ctcgtcgaag agggcagcgt ctcccccgac cgcgaggagc agcaccccgt cctccgccgc
     1081 gtccttccag acaacgctcc gcatacgaat gaaggggcgc gccgcttcat ccttttcccg
     1141 gcggctgatc tcgttcgtga agaggacggc gtccttccct ccgacaactt tttcttcctc
     1201 gtggaagagc gcttcgatgc tccctccggg agatcgcctc agcggagcga ggacgtcttc
     1261 gtagcggttc cttttctccc tcccggcgag cagcaccagg gaacctacgg aagcgcctcc
     1321 gcttggaggt ttgccggtat aaaaaccgga agagcctttt tttgaatagg gggaccatga
     1381 atccggaatt tcgatgtaca gcgagtcgat acgctggtac gtccaggtat tttcaagagc
     1441 ggacgccgcc ccggagaaac ctcccgcgac agcccaggcg atcatgaaga cacagaaacg
     1501 gcgaacagaa aagaatcgca tgccacacac ctccatagcg aatagaatct cccggtcaat
     1561 cgaacagctc ctccagcccc tcaagagagg acgcgagctc aatctccttc ttcactgcgc
     1621 cggcgaagcg ggagagctcc gggtcctcga agagcgagag gctttcttcg aagaaacgcg
     1681 ccgcatcg
//
LOCUS       DAOSWW010000667         1686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62153_length_1686_cov_3.565910, whole genome shotgun sequence.
ACCESSION   DAOSWW010000667 DAOSWW010000000
VERSION     DAOSWW010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62153_length_1686_cov_3.565910"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK442_11780"
     CDS             complement(<1..270)
                     /locus_tag="PK442_11780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO88373.1"
                     /translation="MSTSRSTYRSLFADPSFPLFIILTSSFFLTVFFRISASVILPLE
                     GERLGMSASLVGFISSVHFYAYALMQPVSGILHDRYGPVRVVTCGL"
     gene            complement(441..917)
                     /gene="fliS"
                     /locus_tag="PK442_11785"
     CDS             complement(441..917)
                     /gene="fliS"
                     /locus_tag="PK442_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOO88374.1"
                     /translation="MFWPKNRSRAQETYTENAIRTASREQLLLITYDIGIQSCLAAES
                     ALSNHLYDETNEHLKRAQNVVRELMITLRVNKDNPVTTNLMRLYDFMYTTLIKANTAK
                     DPEKIAEVRAMLEDLKATWEESIVKIAEEYDESKKSEYLNNLPSVQRNGDRFSARG"
     gene            complement(1109..>1686)
                     /locus_tag="PK442_11790"
     CDS             complement(1109..>1686)
                     /locus_tag="PK442_11790"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOO88375.1"
                     /translation="FLKPTEDELYLHFKTIAGAVPGTPVLLYNNPGRTGYSLSAPLVG
                     RLANDVENIVGIKDSSGDMTLTSEFLRLTKTKEKDFKVFGGKDTLIYGALAHGADGAV
                     ATMANFCPELVVSIYEKYVAGDLKGSLEAQFRLNPVRLAMDRASFPVATKDMAKLLGL
                     DVGEPFRPNLSSAGDVLEFMKKRMQQGGLLP"
BASE COUNT          337 a          506 c          471 g          372 t
ORIGIN      
        1 cagaccgcac gtcaccaccc gcaccgggcc gtatctgtcg tgcagaatgc cggagacagg
       61 ctgcatcagc gcgtacgcgt aaaaatgaac gctggagata aaccccacca gcgacgcgct
      121 catgccgagc cgttctccct ccagcggcag aataacggag gccgaaatcc ggaagaagac
      181 cgtcaggaaa aacgatgagg tcagtatgat gaacagggga aaggatggat ccgcaaaaag
      241 cgatctgtag gtcgaacgtg aagtgctcac agcaaagctc ctttgtcgca gtcaatgttt
      301 tgaacggggt acagcatacc atagaaaatg aactgcggga agtcgggaaa catacttata
      361 atgaagagaa ataaccagct tcttccgtgt tgctgctatt cttttccgga gcgtccgcct
      421 cgctggaact tcgcccccct ctacccccgg gccgaaaacc tgtcgccgtt ccgttgcacc
      481 gagggcaggt tgttcagata ctcgctcttc ttgctttcgt cgtactcctc cgctatcttg
      541 acgatgctct cttcccaggt cgccttcagg tcttcgagca ttgcgcgcac ctccgcgatc
      601 ttctccggat ccttcgcggt gttcgccttg atgagcgttg tgtacatgaa gtcgtagagg
      661 cgcatgaggt tcgtcgtgac cggattgtcc ttgttcacgc gcagcgtgat catcagctcc
      721 ctcacgacgt tctgcgcccg cttgaggtgt tcgttcgtct cgtcgtagag atggttcgac
      781 agcgcggact ccgcggccag gcagctctga atgcctatgt cgtaggtaat cagcaggagc
      841 tgctcgcgcg acgcggtgcg aatggcattt tcagtatagg tttcctgcgc ccggctcctg
      901 ttttttggcc agaacatgac aactcgcccc ctcccgcgga gattttcgca aaacgttcaa
      961 agagtttcct cgatgtcttt tattttcggt agagcggagg ggcaggttaa gtctgaacag
     1021 acagcaacag gaagagagtt cacagagggc gtgcccgcaa tttcccgcgg atacgccctc
     1081 tttatcttct tagcgaagat attgtacgct aaggcagcag tccgccctgc tgcattctct
     1141 ttttcataaa ctccagcacg tcgcccgcgc tgctcaggtt cgggcggaag ggctcgccga
     1201 catccagccc cagtaatttc gccatgtcct tcgtggcgac ggggaagctc gcccggtcca
     1261 tggcgaggcg caccggattc agccggaact gcgcctcaag agagcccttc aggtcgcccg
     1321 cgacgtactt ctcgtagatt gagaccacaa gctcgggaca gaagttcgcc atggtggcga
     1381 ccgcgccgtc cgcgccgtgg gcgagcgcgc cgtagatcag cgtgtccttt cccccgaaga
     1441 ccttgaagtc cttctctttc gtcttcgtca gccggaggaa ctcgctcgtc agcgtcatgt
     1501 cgccgctgga gtccttgatg cccacgatgt tctccacgtc gttcgcgagc cgccccacaa
     1561 gaggcgcgga gagcgagtac cccgtcctcc ccgggttgtt gtacagcagc accggcgtac
     1621 ccgggacggc cccggcgatg gttttgaaat ggaggtacag ctcgtcctcc gtcggcttca
     1681 ggaaca
//
LOCUS       DAOSWW010000668         1684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62320_length_1684_cov_2.016575, whole genome shotgun sequence.
ACCESSION   DAOSWW010000668 DAOSWW010000000
VERSION     DAOSWW010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62320_length_1684_cov_2.016575"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PK442_11795"
     CDS             complement(<1..378)
                     /locus_tag="PK442_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88376.1"
                     /translation="MKIIEGMKRLRVIEKRMESQRNAVTEYASKLSTEMPRFQTKEDQ
                     AKEVASLIQSNNDLCAEYLRIKRSIEYTNLKVSVELQGKAYSISDLLVVKRKLADRMV
                     ATYRALNDTVARDRLRNAPKFDGE"
     gene            640..1437
                     /locus_tag="PK442_11800"
     CDS             640..1437
                     /locus_tag="PK442_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO88377.1"
                     /translation="MTNSKGRNTHEEFMNRETPRASLEAKANTSLSELKSMPAWKAVG
                     VCEDPETLKKLLESGVDIEARDHDGRTLLIVAAEQNPNHEIVQLLLDAGADVAFRTKF
                     GVTALGQAARRNPNPKVLKVLIDAGAEIDSRGFQGQTPLMLAAYRITNAESIRLLLDA
                     GADVNAQDNYGETPLILAAASPNSNPEALRVLIDAGANVSHRCASGRTALTYAHEHKV
                     APETMRVLLDAGARIEEKDLGADDDGAVLEQMFADLEEIAFSDMDDE"
BASE COUNT          378 a          462 c          500 g          344 t
ORIGIN      
        1 ctcgccgtcg aatttcggag cattccgcag acggtcccgg gctaccgtgt cgttcagagc
       61 gcggtaggtg gccaccatcc tgtcggcaag ttttctcttg accacaagca ggtcggagat
      121 gctgtacgcc tttccctgaa gttcaacgga cactttcaga ttcgtgtact cgatcgaacg
      181 tttgatcctg aggtactccg cgcacagatc gttgttcgac tggataagag acgccacctc
      241 tttcgcctgg tcttccttcg tctggaaacg aggcatctcc gtggagagtt tcgaggcata
      301 ctcagtaacc gcattccgct gggattccat acgcttttca atgactctca gtcgtttcat
      361 tccttcgatt atcttcatgg ccgagccctc cccggtgtat ttggattcga gattccgcgg
      421 gggaacctcg gtgcgtccga caagtgaaag tatagcgcac gacgacgacc gtttacgtcg
      481 tcgacctgaa tgtagcaaaa aagttctttc gcgttttttc cgggaaggag ccggtaggtt
      541 ggaacggaat cccggccccg caacacgtaa gcggtcgccc tgaatatgta tcatggctgt
      601 gttgtcgatg gacgaggcag tacgaaaggg gatgttctca tgacgaattc aaaaggccgg
      661 aacacgcatg aagaattcat gaaccgggaa actccgcgcg cttccttgga agccaaggcg
      721 aatacctcgc tctccgagct caaaagcatg ccggcgtgga aagccgtcgg agtctgcgaa
      781 gatccggaga cgttgaaaaa gctgctggag tcgggagtgg atattgaagc gcgcgaccac
      841 gacgggcgaa cgctcctgat cgtagccgcg gaacaaaatc ccaaccatga gatagtacag
      901 ctccttcttg atgcaggagc ggacgtagcc ttcagaacga aattcggcgt cacggcgctc
      961 ggccaggccg cccggcgcaa cccgaatccg aaggtgttga aagtcctgat cgacgcgggc
     1021 gccgaaatag actcccgtgg cttccagggc cagactcccc tgatgctcgc ggcctaccgc
     1081 atcacgaatg cggagtcgat acggctgctg ctcgacgccg gggccgacgt taacgcgcag
     1141 gacaactacg gagagacccc gctcatactg gcggcggcat cgcccaactc caaccccgag
     1201 gcgctgcgcg tcctgatcga cgcgggagcg aatgtctcgc accgctgcgc gagcgggaga
     1261 acggcgctga cgtatgcaca tgaacacaag gtcgcccccg aaacgatgcg cgttcttctc
     1321 gacgccgggg cccgcatcga agagaaagac ctgggagcgg acgatgacgg cgcggtattg
     1381 gaacaaatgt tcgccgacct cgaggagatc gccttctcgg atatggacga cgagtgatcg
     1441 aggcgcgtgg ggtccccgat gcgcatcgcc cgcgaacggc ggaatcgcgc catgcgggaa
     1501 tatgaaagaa ggaggatgat gctccatgga tgataacgag ttcgaggatg gtgggcttgg
     1561 cggaatcgga gatgttatag atcgaaacta tttggttatt ggaacattca tcggtcttgt
     1621 ggccttggta ctgaactaca gtgtaggcac gttactttcg tcgaatcaac tgcaactgat
     1681 gaac
//
LOCUS       DAOSWW010000669         1682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62405_length_1682_cov_3.696988, whole genome shotgun sequence.
ACCESSION   DAOSWW010000669 DAOSWW010000000
VERSION     DAOSWW010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62405_length_1682_cov_3.696988"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1566
                     /locus_tag="PK442_11805"
     CDS             154..1566
                     /locus_tag="PK442_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOO88378.1"
                     /translation="MSMQLFILVAYILVLYGISWYATKLIQQGKGGALQYLLAGRNLP
                     MIVVAAMLAGLAIGGASTVGVAEHAYKAGLSAGWYNAAWGMAGILAGIFAAHYFRRME
                     VNTIPEMMERMYGQSARMISVVAQLLIMMVITSLQYVAGGAVLTALMPDVFTFQTGMM
                     VTAVLFVGITFIGGYWGAGLTNVVNIIVIYGGILAALWNSSEVFGGFESIVAALPEGG
                     PWFDFVSGAGFAMVMSWMVVMITQGFSVQAISQIAFAAKNEKAARWGFILGGVIILPA
                     GFLCALFGIIAAAKFPGLERAALALPTIMTNISPMVGGIFLAALWAAGISTAVGLLLG
                     SSTLVMQDVWKRFFPSTVTEKNELLLSRLMVLLFSGVTYFLALTSVGILRTITTALAI
                     TASFTLLILANIFFPKYCKKAAGFWTILASVAVWLAWTLYPQIRVVPHVVYLVWPACL
                     ATFALAYFLGKEPAESIIKR"
BASE COUNT          268 a          514 c          537 g          363 t
ORIGIN      
        1 gcttctccgg gcgggctttt caacgccaac caaccctgcg agggacgtac gaacgccgca
       61 gcaagtttcc acgagacatc agtgaacctc ttttcatatt gccgcaaaat gctatactac
      121 tgggaaattt ctttttccga aggaggaagg tcgatgtcga tgcagctttt cattctggtg
      181 gcgtacattc tggttttata cggtatttca tggtatgcga cgaagctgat ccagcagggg
      241 aaggggggcg cccttcagta tcttctcgcc gggcggaatc tgccgatgat cgttgtggcg
      301 gcgatgctcg cggggcttgc catcgggggc gcatcgacgg tcggcgtggc cgaacatgcg
      361 tacaaagcgg ggctctcggc ggggtggtat aacgcggcgt ggggaatggc gggtatcctc
      421 gcgggcatct tcgcggcgca ctacttccgc cgcatggaag tgaatacgat tcccgaaatg
      481 atggagcgga tgtacgggca aagcgcccgg atgatcagcg tggtggcgca gctcctcatc
      541 atgatggtca tcacctcgct gcagtatgtg gcgggcggcg cggtgctcac ggccctgatg
      601 cccgacgtct tcaccttcca gacaggaatg atggtcacgg cggtcctctt tgtgggcatc
      661 accttcatcg gcggctactg gggcgccggg ctgaccaatg tggtgaacat catagtgatc
      721 tacggcggta tcctcgccgc gctgtggaac tcctccgagg tcttcggcgg cttcgagtcc
      781 atcgtcgccg cgctgcccga gggggggccg tggttcgact tcgtctccgg cgctgggttc
      841 gcgatggtta tgtcgtggat ggtggtcatg atcacgcagg gcttctccgt acaggccatc
      901 tcgcagatcg ccttcgcggc gaagaacgag aaggcggcgc ggtgggggtt catcctgggc
      961 ggcgtcatca tccttccggc cggctttctc tgcgcgctct tcggcatcat cgctgcggcc
     1021 aagttccccg gccttgaacg ggcggcgctc gcgctgccga ccatcatgac gaacatctct
     1081 cctatggtgg gcggcatttt cctcgccgcg ctgtgggccg cgggaatctc cactgcggtg
     1141 gggctgcttc tggggagctc cacgctcgtg atgcaggacg tctggaaacg cttcttcccc
     1201 agcacggtca cggagaagaa cgagctgttg ctctcccgcc tgatggtgct gctcttcagc
     1261 ggcgtcacct acttcctcgc gctcacgtcg gtgggcatcc tgcggacgat cacgacggcg
     1321 cttgccatca cggcctcctt cactctattg atcctggcga acatcttctt cccgaagtac
     1381 tgcaagaagg ccgccggatt ctggaccatc ctcgcctccg tggcggtttg gctggcgtgg
     1441 accctgtacc cgcagatccg cgttgtccct cacgtggtct acctcgtctg gcccgcctgc
     1501 ctggcgacct tcgccctcgc ctacttcctg ggcaaggagc ccgcggagag catcatcaag
     1561 cgctgaaaac gcgcgtaacg cacacggcga ggggccgcct gaggattggc ggtccctcgc
     1621 tgtgtcacct cccccgaagc agcgcgagca cttcgacggc ggcggtgcgc gggaagaagt
     1681 cg
//
LOCUS       DAOSWW010000670         1681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62466_length_1681_cov_3.524600, whole genome shotgun sequence.
ACCESSION   DAOSWW010000670 DAOSWW010000000
VERSION     DAOSWW010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62466_length_1681_cov_3.524600"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK442_11810"
     CDS             <1..415
                     /locus_tag="PK442_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO88379.1"
                     /translation="DVEDYASVALTFSDGTKALVIAGDTMLGGTRNYVEVYANDAVMH
                     CRITPSDVMSTYMLDEEGLENVYISEMLPAKTGWQQPFVAEEHLRGYAAQFQDFIECA
                     ATGRAPLSDFELACETIRVIYAAYLSAEEGRRILL"
     gene            complement(546..1484)
                     /locus_tag="PK442_11815"
     CDS             complement(546..1484)
                     /locus_tag="PK442_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOO88380.1"
                     /translation="MTMTLPKSIFFCEVGPRDGLQNEPATLSVERKVRLIEGVVDAGV
                     RIVEIGSYVNPKAVPQMADTDEVARAIRRMEGVEYRALVANLKGVERALASGVNKVKL
                     TVSASESHAKANLNKTPSELIDGFKSCTEFARSNGAEVSGAISTAFGCPFEGKVPPEK
                     VAEIAERLHDLGITELSLSDTTGMANPRQVYDLATSMQEMLPDVRWFLHFHNTRGMAL
                     ANTLAGLQAGIVRYDASLAGLGGCPFAPGASGNVASEDMIHMLHEMGVDTGIDLDKLL
                     DLARELRGWVGHDTDSALLKAGPCCRPIPRDGAAKQ"
BASE COUNT          342 a          463 c          506 g          370 t
ORIGIN      
        1 cgacgtcgag gattacgctt cggtagccct tactttcagc gatgggacga aagcgctcgt
       61 catcgccggc gataccatgc tggggggaac gcgcaattac gtagaggtgt acgccaacga
      121 cgccgtcatg cactgccgga ttacgccgtc cgatgttatg agcacttata tgcttgacga
      181 agaggggttg gaaaacgtct atatctccga gatgctcccc gcaaagacgg gctggcagca
      241 accttttgtt gccgaggagc accttcgggg gtatgcggcg cagttccagg acttcatcga
      301 gtgcgcagct acgggaaggg ctccactttc ggattttgaa ctggcctgcg aaacgatccg
      361 cgtcatttat gcggcgtatc tctctgccga ggaggggcgt cgcattctgc tgtagacgcc
      421 attcggaggc gaccgtaaac tcctgacaga gcatttcaca gtgaaagcga gtcgcctttt
      481 cgtctgcgtc tcgccttcac tggattggta gggatatgcg gtctttctgc ggcgtgcggc
      541 tgctctcact gcttcgcggc gccgtcccga gggatggggc ggcagcacgg acccgctttc
      601 agcagcgcgc tgtccgtgtc gtgtccaacc cacccgcgga gttccctcgc aaggtcgagt
      661 agtttgtcga ggtcgatccc cgtgtcgacg cccatctcat gaagcatgtg aatcatgtcc
      721 tcggaagcga cgtttccgga agcgccaggg gcgaaggggc aaccgccgag gcccgcaagc
      781 gacgcgtcgt aacgtacgat cccggcctgg agaccggcaa gcgtatttgc aagggccatt
      841 ccgcgcgtat tgtggaagtg aaggaaccat cggacgtcgg ggagcatctc ctgcatagag
      901 gttgcgagat cgtaaacctg ccgcgggttc gccatgccgg tggtatccga aagggagagt
      961 tcggtgatgc cgaggtcgtg cagacgttca gctatttccg caaccttttc gggaggtacc
     1021 ttcccttcaa aggggcagcc gaaggcggtg gagatggctc ccgaaacttc ggctccgttc
     1081 gaacgggcga actccgtgca gcttttgaac ccgtcgatca gttcggacgg tgttttgttg
     1141 aggttcgcct tcgcatgcga ttcgctggcc gacacggtga gtttcacctt gttcactccc
     1201 gaagcgaggg cgcgttccac tcccttgaga ttggcgacaa gcgctcggta ctccacacct
     1261 tccatccggc ggatggcgcg tgctacctcg tcggtatcgg ccatctgggg aaccgccttt
     1321 ggattgacgt acgagcctat ttccacgatc ctgaccccgg cgtcgacgac gccctcgatc
     1381 aagcggactt tccgctcgac ggagagcgtg gccggctcgt tctgaagacc gtcgcggggg
     1441 ccgacttcac aaaagaaaat gcttttgggc agtgtcatgg tcattgctat ccctcctttg
     1501 catactgatg ataaacctct ttccgggaca acagtactca aaaaaccaat tcctgtcaaa
     1561 cggtttatat ttccgttcaa tgggaattta gagtgatgga gaagtgccga tacaacgaaa
     1621 gtgtttcccg ggtgacagaa aaaaagaaga ggaagtattg acaaaggcga cggcgataga
     1681 t
//
LOCUS       DAOSWW010000671         1679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62584_length_1679_cov_3.751232, whole genome shotgun sequence.
ACCESSION   DAOSWW010000671 DAOSWW010000000
VERSION     DAOSWW010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62584_length_1679_cov_3.751232"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PK442_11820"
     CDS             complement(<1..272)
                     /locus_tag="PK442_11820"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HOO88381.1"
                     /translation="MRVIRRTPPDYRTMPWKNGGGITHEMFIYPEDATLQEEEFLWRL
                     SSANVGESGPFSTFPGYDRVLFFLSGNALDLTVNGETVSLRSPFDSL"
     gene            complement(311..>1679)
                     /locus_tag="PK442_11825"
     CDS             complement(311..>1679)
                     /locus_tag="PK442_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOO88382.1"
                     /translation="VHGTFNSILNAARQRLGVGEKENLAGILFVSSGLGGMSGAQPKA
                     VEIAGGVGIIAEVDASRIETRHSQGWVAKVTDSCKEAFDLAAEACVKKQPLSIAYHGN
                     IVDLLQYAAEHEIGIPLLSDQTSCHAPYDGGYCPQGLTFDERTKMLSKNPEEFRRLVD
                     ISLRKHYEFIKTLVDRGTYFFDYGNSFMRAVFDAGVPEICKNGENTYDGFVWPSYVED
                     IMGPVLFDYGYGPFRWVCLSGDPEDLRKTDHAAMECIDPDRRSQDYDNWLWIRDAEKN
                     ALVVGTQARILYQDAEGRTRIALKFNEMVRKGEIGPVMLGRDHHDVSGTDSPYRETAN
                     IKDGSNICADMATVCFAGNAARGMTLCALHNGGGVGIGKSVNGGFGLVLDGAERTDEI
                     IRSAMMWDVLGGVARRAWARNPNAMEVSREVNRNYPDSYHITLPYSVEEESVKNAVDA
                     LFKAK"
BASE COUNT          290 a          509 c          504 g          376 t
ORIGIN      
        1 agcgagtcga aaggagaacg gagggagacc gtctctccgt tcacagtgag atccagggcg
       61 ttcccggaga gaaagaagag tacgcggtca taccccggaa acgtcgagaa cggacctgat
      121 tcgcccacat tcgcggaact cagccgccaa aggaactctt cttcttgaag cgttgcatcc
      181 tccgggtaga tgaacatttc atgcgtgatg cctccgccgt ttttccacgg catggtccga
      241 tagtccgggg gagttcttcg tatgacgcgc atactcttca cctctcgtta ttctctcgta
      301 gtgtgtgcct ttattttgcc ttaaagagcg cgtcgacggc gttcttcacg ctctcttcct
      361 ccacgctgta cggaagcgtg atatggtagc tgtccgggta gttcctgttc acttcccggc
      421 tgacctccat cgcgttcggg ttccgggccc aggcgcgccg ggctacgccg ccgaggacgt
      481 cccacatcat cgcggacctg atgatctcgt cggtgcgctc cgccccgtcg aggacgagtc
      541 cgaatcctcc gttgaccgac ttgccgatcc ccacgccgcc gccgttgtgc agcgcgcaga
      601 gcgtcatgcc gcgtgctgcg ttgccggcga agcagaccgt cgccatgtcc gcgcatatgt
      661 tgctgccgtc cttgatattc gccgtttcgc ggtaggggga gtctgtcccg gagacgtcgt
      721 gatggtcgcg gcccagcatc accgggccta tttctccctt tcgcaccatt tcgttgaatt
      781 tgagggcgat gcgggtgcgt ccctcggcgt cctggtagag tatccgggcc tgcgttccca
      841 ccacaagggc gttcttctcg gcatcgcgaa tccagagcca gttgtcatag tcctgtgagc
      901 gccggtcggg gtcgatgcac tccatcgccg cgtggtcggt cttgcgcaga tcctcagggt
      961 cgccgctgag gcagacccag cggaacggac cgtagccgta gtcgaagagg acgggaccca
     1021 tgatatcctc cacgtaagag ggccatacaa agccgtcgta cgtgttctcg ccgtttttgc
     1081 agatctcggg gactcccgcg tcgaagaccg cgcgcatgaa cgagttgccg tagtcgaaga
     1141 agtatgttcc cctgtcgacg agcgtcttga tgaactcgta gtgcttcctc agggatatat
     1201 ccacaaggcg ccggaactcc tccgggttct tcgagagcat cttggtgcgc tcgtcgaagg
     1261 tcagtccctg cgggcagtag cctccgtcgt agggagcgtg gcaggaagtc tggtcggaga
     1321 gcagaggaat cccgatctcg tgttcggccg cgtactgtaa cagatcgacg atgttcccgt
     1381 gataagcgat cgaaagaggt tgttttttca cgcacgcctc ggcggcgaga tcgaaagctt
     1441 ccttgcagct atctgtgact ttggcgaccc atccttgact gtggcgcgtt tcgatgcgcg
     1501 aagcgtccac ctcggcgatg atgccgacgc cgcctgcgat ttccacggcc ttcggctgcg
     1561 cgccgctcat ccctccgagt ccggaggaga cgaagaggat tcctgcgaga ttttcctttt
     1621 cacccacgcc gagccgctgc cgggcggcgt tgagaatgct gttgaacgta ccgtggacg
//
LOCUS       DAOSWW010000672         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62691_length_1678_cov_2.166359, whole genome shotgun sequence.
ACCESSION   DAOSWW010000672 DAOSWW010000000
VERSION     DAOSWW010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62691_length_1678_cov_2.166359"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1678)
                     /locus_tag="PK442_11830"
     CDS             complement(<1..>1678)
                     /locus_tag="PK442_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOO88383.1"
                     /translation="GTTAVAYSPSLSGAVDQLSPVSEANARGAVFTRSEVVDFILDLV
                     GYTDDQPLYTKRLLEPSFGGGAFLLPAVRRLLSAWKRFRTSSPTPEELGESLLAVELH
                     GETYRDTRGETIRLLEREGMKPGEAVTLADRWLSQGDFLLAPLSGRFDFVVGNPPYVR
                     QEMIPAPLLSEYRRRYRTLYDRADLYIPFIERSLSLLTKGGVLGFICSDRWMKNRYGG
                     PLRSLVASNYRLKIYVDMTDVGAFHSDVSAYPAITVIGREPSGSTRIAYRPEIDRETL
                     TSLASEFRSPGLPKISGSVREIDGVTSGAAPWLLEPSDRTDLIRRLEENFPSLEEAGC
                     SVGIGVATGADKAFIGKYDALDVEPDRKLPLVTTKDIVSGEVRWLGRGIVNPFADGGG
                     LVDLKAYPRLGRYLEERRDVIARRHCAQKSPDNWYRTIDRITPSLTSRPKLLILDIKG
                     EAHIVYEQGELYPHHNLYYVTSNEWELRALQAVLLSAISRLFVTTYSTKMRGGYLRFQ
                     AQYLRRIRIPRWSDVSGVLRKELTEAAIARDIHACNNATFRLYGLNGEERA"
BASE COUNT          351 a          488 c          503 g          336 t
ORIGIN      
        1 gccctttctt cgccgttcag gccgtacagc ctaaaggtcg cgttgttgca ggcgtggata
       61 tctcttgcga tcgccgcttc cgtaagttct tttcttaaaa ctccggagac gtccgaccat
      121 cgcggaattc tgatacggcg tagatattgc gcttgaaaac gcagatatcc gccgcgcatc
      181 ttcgtcgaat aggtcgttac gaaaagacgg ctgatcgccg agaggagaac ggcctgtaac
      241 gcgcgcaact cccactcgtt cgatgtgacg tagtacagat tatggtgcgg atacagctct
      301 ccctgttcgt agacgatgtg cgcctcgccc ttgatgtcga gaatcaggag cttcggcctc
      361 gacgtcagcg acggtgtgat gcggtcgatc gtgcgatacc agttgtccgg ggatttttgc
      421 gcgcagtgac ggcgagcgat cacgtcgcgt ctctcctcaa gataacggcc caaacggggg
      481 tatgccttca aatcgaccag gccgccgccg tccgcgaacg gattgacgat ccctcggccg
      541 agccaacgga cctcgccgga gacgatatct ttcgtcgtaa ccaaaggaag tttcctgtcc
      601 ggttctacgt cgagagcgtc gtacttgcca atgaacgcct tgtcggctcc tgtcgccact
      661 ccgatgccga cggagcatcc ggcttcctca agagaaggga agttctcttc cagacgtcgg
      721 atgagatccg tccggtccga cggctccaac agccacggag cggcgccgct tgtcacgccg
      781 tcgatttccc gcacggagcc ggagattttt ggtagcccgg gcgaacgaaa ttccgatgca
      841 agagaggtca gtgtttcgcg gtcgatttcc ggccgatagg cgatccgcgt cgagccggaa
      901 ggttcgcgcc cgatgaccgt gatcgccgga tatgccgaca catcggaatg gaaagcgccg
      961 acgtccgtca tgtccacata gattttgagc cggtagttgc ttgccacaag gctgcgtaga
     1021 ggtccgccgt agcggttttt catccacctg tccgagcaga tgaatcctaa aacgccgccc
     1081 ttggtcaaca gggagaggga acgttcgatg aacgggatat agaggtcggc gcgatcataa
     1141 agcgtcctgt agcgcctgcg atactccgag agcaagggag ccggtatcat ctcctggcgc
     1201 acatagggcg ggtttcctac cacgaagtcg aatctgccgg aaagaggcgc gagcagaaag
     1261 tccccttgcg acaaccagcg gtctgcaagc gtcaccgctt cgcccggttt catcccttcc
     1321 cgctccagca atcggatggt ttctccgcgt gtatcgcggt atgtctcgcc gtggagttca
     1381 acggcgagaa gagattctcc aagctcctcc ggagtcggag aggaagtgcg aaagcgtttc
     1441 catgccgaga gcagtctccg tacagccggc agcaaaaaag ccccgccgcc gaacgagggc
     1501 tccagaaggc gcttcgtgta aaggggctga tcgtccgtgt agccgacgag atcgagaata
     1561 aagtcgacga cctccgaacg cgtgaacacc gccccgcgag cgttcgcctc agagaccggg
     1621 gatagttggt cgacggcgcc ggagagcgaa ggggagtacg caactgctgt agtgccgc
//
LOCUS       DAOSWW010000673         1677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62709_length_1677_cov_3.622072, whole genome shotgun sequence.
ACCESSION   DAOSWW010000673 DAOSWW010000000
VERSION     DAOSWW010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62709_length_1677_cov_3.622072"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PK442_11835"
     CDS             <1..967
                     /locus_tag="PK442_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOO88384.1"
                     /translation="AAFASARLIRDAGDNLPYISTYCPAVIRLIQINHPELVGRILPV
                     ESPLETAVSIWREETGAEESVTLVSPCPAKATLVRNPVGREQSSLEYVVSVRKVIRDL
                     LACNVKVTGTKTKAISRRWLLWSISGGESRHISSFFDKGFTSISVSGLRNTQDLLNEL
                     ELGRLAGVDFIECRACDLGCIGGTGTYESRFLSQLRLDNIEAEWMPSGEEMEQIRSWY
                     DKGIWRLAAPVTAKERLPLSKDIKEAMAKLREMDAIYAELPHIDCGACGRPSCRALAE
                     DIVRGQGEVTDCVFKLRERITNLSGEIKNLSSKLPHTLHPGRKRR"
     gene            986..1300
                     /locus_tag="PK442_11840"
     CDS             986..1300
                     /locus_tag="PK442_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOO88385.1"
                     /translation="MSLKRPGRRFSLRDAERELAGAISGDLLSFIMAEAKEGWLWITI
                     QVHLNVAAVAVLKDVPFILVASGRAPEQALVERCAAEGISLAVTKLPAFEAAGALREL
                     AR"
     gene            1303..>1677
                     /locus_tag="PK442_11845"
     CDS             1303..>1677
                     /locus_tag="PK442_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO88386.1"
                     /translation="MLSHFWVDLHLHTVLSPCGELEMGAPEIVAAYREAGIALCAVTD
                     HNDIANVPAIRGAASGNPVVLFGMEVQTAEDIHIVVLFPDDRTAEGYREWLWREMPQR
                     ANDPDIFGDQLVIDSENAILDEQ"
BASE COUNT          325 a          486 c          543 g          323 t
ORIGIN      
        1 cgccgctttt gcctcggcgc gtctcatccg cgatgcggga gacaaccttc cgtatatttc
       61 cacctactgt ccagcggtga tccgccttat ccaaataaac catcccgaac tcgtcggacg
      121 tattctgccc gtggaatcgc cgcttgagac ggcggtttcc atatggaggg aggagaccgg
      181 agccgaagag agcgtcacct tggtttcgcc ctgtcccgcg aaggcgacgc tcgtgcgcaa
      241 tccggtcggg agggagcaga gttccctcga gtacgtggtg agcgtacgga aggtgatacg
      301 ggacctcctg gcctgcaacg tgaaggtgac gggcacgaag acgaaggcga tcagcaggag
      361 atggcttctt tggtcgatct ccggcggcga gtcgcgtcac atctcctctt tcttcgacaa
      421 ggggttcacg tcgatctccg tctcgggctt gcggaacacg caggacctcc tcaacgaact
      481 tgagctcggc cgccttgcgg gcgtcgactt catcgagtgc cgcgcgtgcg acctcggatg
      541 tatcggcggc acggggacgt acgagtcccg ttttctctcc cagctccgcc ttgacaacat
      601 cgaagccgaa tggatgccct cgggggagga gatggagcag atccggtcgt ggtatgacaa
      661 gggcatctgg cgtcttgccg cgccggtgac tgcaaaggag cgtctgccgc tctccaagga
      721 tatcaaagag gcgatggcga agctgcgcga gatggacgcc atctacgcgg agcttcccca
      781 tatagactgc ggagcgtgcg gccgcccgtc gtgcagggcg cttgcggagg atattgtccg
      841 gggacaggga gaagtaaccg actgcgtttt caagctccgt gagcggataa ccaatttgag
      901 cggcgagatc aagaatctct cctcgaaact gccccatacg ctccaccccg gaagaaaacg
      961 gaggtgacat tgatgaaaat acgggatgtc attgaaaaga ccggggcgac ggttctcgct
     1021 ccgggatgct gaacgcgaac ttgccggagc gatctccggc gacctcctga gtttcatcat
     1081 ggctgaagca aaggaggggt ggctctggat cacgatccag gtgcacctca acgtcgctgc
     1141 ggtggctgtg ctgaaggatg tgccctttat cctcgtcgct tcgggaaggg cgccggagca
     1201 agcgctggtc gaacggtgcg ccgccgaggg tatttccctc gccgtgacga agctccccgc
     1261 ctttgaagcc gcaggggcgc tccgggagct tgcgcgctga gcatgctctc gcacttttgg
     1321 gtagacctgc acctgcatac cgtcctctcg ccgtgcggcg agctggagat gggagccccg
     1381 gagatcgtcg ctgcctacag ggaagcaggt atcgctctct gcgcggtgac cgaccataac
     1441 gacatagcga acgttcccgc aatcaggggc gcggcgtctg gaaaccctgt cgtcctcttt
     1501 gggatggagg tgcagacggc ggaggatatt cacatcgtgg tgctcttccc cgacgaccgg
     1561 actgcggaag ggtaccggga atggctctgg cgggagatgc cccagcgtgc gaacgatccg
     1621 gacatcttcg gcgaccagct cgtgatcgac agtgaaaacg cgattctgga cgagcag
//
LOCUS       DAOSWW010000674         1675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62851_length_1675_cov_2.874074, whole genome shotgun sequence.
ACCESSION   DAOSWW010000674 DAOSWW010000000
VERSION     DAOSWW010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62851_length_1675_cov_2.874074"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..1335
                     /locus_tag="PK442_11850"
     CDS             367..1335
                     /locus_tag="PK442_11850"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOO88387.1"
                     /translation="MPMYSDALLVKAAELYYYSRRSQAEIGRTLGVSTPTVSRILQEA
                     MERGVIEVRVRDILVRSSETEATLKRTFGLVDAVVVDMPQDADLNFLRKLLGKKASEL
                     LPRFCERGSTVGIGCGVTVREMIESLDRSRRFPDLRIVPLMGGWGKEEPERETNRLAS
                     SMGSILGCAFFYLLAPAIVSSPAVRDVFLAEPQIQLTTKLWNTVDTAIFSIGPELDAP
                     AYSYIPREYLDLEKAAKAGGVGDVLGRIIDENGKELALPFNEHLMSIPFATLLKIPRR
                     VGIGGGAQKCRGVLAALRGGLVNILVTDYETGSYILQMEAMQRDSV"
     gene            1322..>1675
                     /locus_tag="PK442_11855"
     CDS             1322..>1675
                     /locus_tag="PK442_11855"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOO88388.1"
                     /translation="MTVFETMFKVKKPILGMVHFPPLPGSPLYDGSAGMRKIRDTAMR
                     DAEALVKAGFDGIVFSNEGDRPYLSSVDHVTVAAMSRVISETAATFNVPFGLSVLADP
                     EAAVAIGAAVEADFVR"
BASE COUNT          352 a          463 c          508 g          352 t
ORIGIN      
        1 ccaaacctgt cgaacggacg gcgctgctga agatgatcaa gcgctactgc gaaagaaagg
       61 atgtctaagc gctcagcccc tctctttgaa atgaagggaa attgcccctc ttccgtgaaa
      121 gcagtctttc ccgggtacta acgaagggga ttttgtggac gagagaacct ttcgcgttgg
      181 cccctccaaa acatgttgtg cgcgacttcc gatacgctcc cctccgtggt acacagacgg
      241 aggggagcgt cgtttctaca acctccactt tcaattcact tccgagcgat tgacaatttg
      301 cggaactctc ctatacttat acagaagaga gatagaaaat tatttcagga atttttttca
      361 agggggatgc cgatgtattc ggacgcgctt ctggtaaagg cagccgaact ctactactac
      421 tcccggagat cacaggcgga gataggaagg actctcgggg tttccacccc cacagtgtct
      481 cggattcttc aggaggcgat ggagcgcggc gtcatcgaag tccgcgttcg cgacatcctt
      541 gtccgctcct cggagaccga ggcgacgctg aagagaacct tcgggcttgt cgacgcggtg
      601 gtggtcgaca tgccccagga tgcggacttg aactttcttc ggaaactgct tggaaagaag
      661 gcgagcgaac tgctgccccg cttctgcgag agaggaagta ccgtcgggat agggtgcggc
      721 gtcaccgtac gcgagatgat cgagtcgctc gaccgctcgc gccgcttccc cgatctccgc
      781 atcgtcccgc tcatgggagg gtgggggaag gaagagccgg agcgggagac gaaccgcctc
      841 gccagttcca tggggagtat tcttggatgc gctttcttct atcttctcgc cccggctatc
      901 gtcagttcgc ccgccgttcg tgacgtcttt ctggcggagc cgcagatcca gttgaccacg
      961 aagctatgga ataccgtgga tacggcgatc ttttccatcg ggccggaact cgacgccccg
     1021 gcgtattcct acataccgag ggagtatctt gacctcgaaa aagccgcgaa agccggaggg
     1081 gtgggcgacg tgctcggcag aatcatcgac gagaacggga aggagcttgc cctgcccttc
     1141 aatgaacacc tgatgagtat tcccttcgcg acgttgttga aaatccctcg gagggtcggt
     1201 atcggaggag gagcgcaaaa atgccgcggc gtcctcgcgg cgctccgcgg ggggcttgtg
     1261 aacatactgg tcaccgacta cgagacggga tcgtacatac tgcaaatgga ggcgatgcaa
     1321 cgtgacagtg tttgaaacga tgttcaaggt gaagaagcct attttgggga tggtgcattt
     1381 cccgccgctt cccggctctc ctctgtatga cggaagcgcg ggaatgagga aaattcgcga
     1441 taccgcgatg cgcgacgccg aagcgctcgt caaggcgggc ttcgacggaa tcgttttcag
     1501 caacgaaggc gacaggccgt atctctcctc cgtggaccac gtgacggtgg cggcgatgag
     1561 cagagtgatt tccgagactg ccgccacgtt taacgtcccc ttcggtctct ccgtactggc
     1621 cgatccggag gcggccgtgg cgatcggcgc ggccgtggag gccgatttcg tccgc
//
LOCUS       DAOSWW010000675         1675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_62857_length_1675_cov_2.724691, whole genome shotgun sequence.
ACCESSION   DAOSWW010000675 DAOSWW010000000
VERSION     DAOSWW010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62857_length_1675_cov_2.724691"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PK442_11860"
     CDS             complement(<1..525)
                     /locus_tag="PK442_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88389.1"
                     /translation="MITKPVLGVDFGTTKTYLSKCPGDDPSPTSVDFGDGRDGVPSAI
                     LYRKEKEPLIGQSALDEYGEATAWERASFRLVTNFKADLARSEEARAAASDFLSALLA
                     DARKQRLDVEPATRRVLFGIPSEADDAFRAALEATAERAGFGAPFLVDEPKGALLYHM
                     KQRDISVDKALGGVL"
     gene            complement(522..>1675)
                     /locus_tag="PK442_11865"
     CDS             complement(522..>1675)
                     /locus_tag="PK442_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88390.1"
                     /translation="PGRKTLALRRSRRYSIGIKRKRGCVLMGRRRKTPVITTKEEEEI
                     RRRAVEKGVNVYFAYLPPNGVPEQRALESAVRRAEAAASEELLPALLDSVADEPLPPE
                     RRSLLLLRMAERLPTLVLPEPELLSEIPPLRAAAASAFGTIAGMAFLPSLTWILLGNR
                     AAGVFIGAPLGALFGTLAALLLPRSRKLLLLLAAILGLATVREVIRFLGGGAIFFKGW
                     SLLREKSGSLKGLALFPLLLATLLLLGARETRFDRKRHEESVRRAFASWTEGAARVAL
                     LAASPSAPLQKDEEEAIPLLASRILSLEGACCEDLTAGVSELAAEVRNLGYSDENPAV
                     LFRWNEEAAGKYTSFGAVEPGDAVRVEREPLFRNGELLAKGLVRKVRRS"
BASE COUNT          307 a          553 c          539 g          276 t
ORIGIN      
        1 gagcacgccg ccgagcgcct tgtccaccga aatgtcgcgc tgcttcatgt ggtagagcag
       61 cgctcccttc ggttcgtcga cgagaaacgg cgcgccgaaa cccgctcgtt cggcggtcgc
      121 ctccagggcg gcgcggaatg cgtcgtcggc ctcgctggga atgccgaaga ggacgcgccg
      181 cgtcgccggc tccacatcaa gccgctgctt ccgcgcgtcg gcgaggagcg cggagaggaa
      241 gtcgcttgcc gctgcgcgag cctcctcgga acgtgcaaga tccgccttga agttggtgac
      301 aaggcggaac gaggcgcgct cccatgcagt cgcctcgccg tactcgtcga gcgcgctctg
      361 gccgataagc ggttccttct ccttccggta gaggatggcg gaaggaacgc cgtcccttcc
      421 gtcgccgaaa tccacgctcg tcggcgacgg atcgtccccg gggcactttg agagatacgt
      481 cttcgtggtg ccgaagtcaa cgccaagaac cggcttcgtg atcatgagcg ccgcaccttt
      541 ctgacgagcc ccttcgccag cagttcgccg ttccggaaga gcggctctcg ctccacgcgc
      601 accgcatcgc caggctccac cgcgccgaag gatgtgtatt ttcccgcggc ctcttcattc
      661 catcggaaga gcacggccgg attctcatcg gagtagccca ggttgcgcac ctccgcggca
      721 agctcggaga cgcccgcggt caggtcttcg cagcaggcgc cttcaaggga gaggattctc
      781 gacgcgagga gcggaatggc ctcctcttcg tccttctgca gaggagcgga cggcgacgcc
      841 gcgagcaggg cgacgcgcgc cgccccctcg gtccatgatg caaaggcacg gcgaacggac
      901 tcctcgtgac gtttccggtc gaaacgggtc tcgcgcgctc ccaggagcaa gagcgttgcg
      961 agaagcagcg ggaaaagcgc gagccccttc aacgacccgc tcttttcgcg cagaagcgac
     1021 caccccttga agaagatcgc gccgccgccg aggaagcgga tgacctctct tacggtcgcg
     1081 agccccagaa tggcggccag cagcaggagc agcttccggc tccgggggag caggagcgcc
     1141 gcaagggttc cgaagagcgc gccgagcgga gccccgatga agacccccgc ggcccggttg
     1201 ccaaggagaa tccaggtaag cgaaggcaaa aacgccatac cggcgatcgt tccgaaagcg
     1261 gatgccgccg ccgcccgaag tggaggaatc tctgagagca actcgggctc cggaaggacg
     1321 agcgtgggaa ggcgctctgc catgcgcagc agaagcagcg aacgccgctc cggagggagc
     1381 ggttcgtcgg cgacggagtc aagaagcgca gggagcagct cctccgacgc cgcagcctcg
     1441 gccctccgga cggcgctttc aagggctctc tgctccggaa cgccgttcgg cggcaagtac
     1501 gcgaagtaga cgttgactcc cttttcgacg gcgcgacgac ggatctcctc ttcttctttt
     1561 gttgtaatca caggcgtttt tcttctcctg cccatcagaa cgcaccctct ttttctcttg
     1621 attccaatag agtagcgcct cgaccgtcga agagcaagcg tttttcggcc ggggg
//
LOCUS       DAOSWW010000676         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_63030_length_1672_cov_2.854051, whole genome shotgun sequence.
ACCESSION   DAOSWW010000676 DAOSWW010000000
VERSION     DAOSWW010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63030_length_1672_cov_2.854051"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..>1672
                     /locus_tag="PK442_11870"
     CDS             318..>1672
                     /locus_tag="PK442_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOO88391.1"
                     /translation="MQKQFKGLTSQEVLESRRTHGENVLTPPVRESWWKLYLEKYDDP
                     VIRILLIAACIAFLVGLAHNEYVEAVGIVVAVFLATTMAFVNEYKAGREFDILNQVND
                     DVEFKVIRDGEYRSVSKKELVVGDILLLETGEEVPADAVVEEAVALQVDESTLTGESK
                     AVSKFSEDDPRGAEKREAATYPPHRVYRGTMVQDGHGVFCIESVGDATEIGKTAREAS
                     AVSEERTPLSRQLDGLSKVIGVVGFGIAAVLFAVLSVHAALVGEIVMERGQWIFALIL
                     IVSVLVALVRVWLPIVYDFFDVIRRPKTRPGWLQQEGITAWLKPLLYGAVFFALASGA
                     AWTLGALPSSPSNFLDADAGRRFLQFFMIAITLIVVAVPEGLAMSVTLSLAYSMRKMT
                     ASNNLVRKMHACETIGAATVICTDKTGTLTKNKMHVHEVRVPSLETSPGLLYEAMAVN
                     ST"
BASE COUNT          304 a          474 c          568 g          326 t
ORIGIN      
        1 ccctctcttc gcacagagcc cgcacaaggt cctcggtgcg cacggggatg aacgagcccc
       61 gctctttctg ttcctccatc gtaactcctc ctgtactcgg cttttctgtg cggacgtccc
      121 gctgcggggc gcatccgcgc agaaagtata gcacggcgtt cacgtttctt caggcgtttc
      181 gagaaggggg tatttcggtc gcgcattctc ttcggtgacg aagaagcgcg ccctgaaagc
      241 gtataatgga accgttgcgc aaagagacgg gaagacggcc ttcacaggtc gtccgcgtgg
      301 atatttggag gtgtgctatg cagaaacagt tcaagggttt gacctctcag gaggttctgg
      361 agagcaggag aacgcacgga gaaaacgtgc tcaccccgcc ggtgcgggag tcatggtgga
      421 agctgtacct ggagaagtac gacgatccgg tgatccgtat tttgctcatc gcggcgtgca
      481 tcgccttcct ggtggggctt gcccataacg agtacgtgga ggccgtcggt attgtcgtcg
      541 cggtgttcct cgccacgacg atggcctttg tcaacgagta caaggcgggc agggagttcg
      601 acatactcaa tcaggtgaac gacgacgtcg agttcaaggt gatccgggac ggcgagtacc
      661 ggagcgtttc gaagaaggag cttgtcgtgg gggatatcct gctgctggag acaggggagg
      721 aggtcccggc ggacgccgtc gtcgaggagg ccgtcgcgct gcaggtcgac gaatccacgc
      781 tgacgggaga gtcgaaggcc gtctccaagt tttccgagga cgatccgagg ggcgccgaaa
      841 aacgagaggc ggccacctac ccgccgcacc gcgtgtacag gggaacgatg gtgcaggacg
      901 ggcacggggt cttttgcatt gagagcgtcg gcgacgccac ggagatcggg aagacggcca
      961 gggaggcctc ggcggtttcc gaggaacgca cgccgctgag ccgccagctc gacggcctga
     1021 gcaaagtgat cggcgtcgtc ggcttcggca ttgcggcggt gctcttcgcc gtgttgagcg
     1081 tccacgcggc gctcgtggga gagatcgtca tggagcgagg gcagtggatc tttgcgctca
     1141 tcctgattgt ctcggtactt gtcgcgctgg ttcgtgtgtg gctgccgata gtctacgact
     1201 tcttcgacgt aatccggcgg cccaagacgc ggccggggtg gctgcagcag gaagggatca
     1261 cggcgtggct gaagccgctg ctctacggcg cggtgttctt cgcgcttgca tcgggcgctg
     1321 cgtggacgct tggcgcgttg ccctcgtctc cctcgaactt cctggacgcc gacgcagggc
     1381 ggcgattcct ccagttcttc atgatcgcca ttaccttgat cgtggtcgcc gtccccgagg
     1441 ggctggcgat gagcgtcacg ctctcgctgg cgtacagcat gcggaagatg accgcctcga
     1501 acaacctggt gcgcaagatg cacgcctgcg agaccatagg cgccgcaacg gtaatctgca
     1561 ccgacaagac cggcacgctg acgaagaaca agatgcacgt gcacgaggtg cgcgttccct
     1621 ccttggagac ctctcccggg ctgctgtacg aggcgatggc ggtgaactcg ac
//
LOCUS       DAOSWW010000677         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_63345_length_1667_cov_3.253722, whole genome shotgun sequence.
ACCESSION   DAOSWW010000677 DAOSWW010000000
VERSION     DAOSWW010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63345_length_1667_cov_3.253722"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..910
                     /locus_tag="PK442_11875"
     CDS             191..910
                     /locus_tag="PK442_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88392.1"
                     /translation="MNTLKRISLGLSAVLCILFIATALPVFAEVQVFIDAHTVRVYDP
                     VLKRQSILNDFRTSGYRVHNHLALVWSEDAAWVYDVRTQQWLSESNFRTLLGSLSDEY
                     ALVWNENEAAIFDAKKQEWIRSDYIPWKLTGASLSRGMTILTGEEGLVVYDPVLKKWL
                     SSQFFPYGALKKAQAGDVLAAAWSDNDLVLYDMTIHDWVVKEGISPQACIIDGYKASI
                     FTADTVYTYDAVSHRWSSKNR"
     gene            949..>1667
                     /locus_tag="PK442_11880"
     CDS             949..>1667
                     /locus_tag="PK442_11880"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOO88393.1"
                     /translation="MNGRRNISAFSVLMLLTLLALPGRAFAASMIVAVQPFLAQGTSF
                     HLGATVGDIVSTSLAGNRDITLVERSRLAEVARQQRLAMSGVVETATAAKAGKLVGAR
                     YFILGAVSRFGALLVATARLVDVESGRVLASFERTSKQGEDETTLVSRNLASDILAFI
                     SGDAPAPGDPTSDYKYYLYEALGYYNMGEYRKSLPFWEKMTQLSPRNGLLRFIVAGLH
                     FQTRRYSDALLAAQQAVTWEPS"
BASE COUNT          344 a          500 c          514 g          309 t
ORIGIN      
        1 gtttcgccgg aggcagggcg gagatcgacg tttcctattc cggttcggcc aggcaactcc
       61 tcgcagccct cctcgccaaa cgcccggacc tgaaggagga gaccgtcgag ggaaacacgc
      121 tccgtcttgt cggggaatag gggaataaat gaagaagttc agaaacacac atgaagggag
      181 ctggtatcct atgaatacac tgaaacggat atctctcgga ctttccgcag tcctctgcat
      241 actgtttatc gcgacggcgc ttccggtttt cgccgaagta caggtcttca tcgacgcgca
      301 caccgtgcgg gtgtacgacc cggtgctgaa acgccagtcc atcctgaacg acttccgtac
      361 gagcggatac cgcgtccaca accatcttgc cctcgtctgg agcgaggacg cggcctgggt
      421 ctacgacgtg cgcacccagc agtggctctc cgagagcaac ttccggacgc ttctggggtc
      481 cctctccgac gagtacgcgc tcgtctggaa cgagaacgag gccgcgatct tcgacgcgaa
      541 gaagcaggaa tggatccgga gcgactacat tccatggaaa ctcaccggcg cgtcgctttc
      601 gcgcggaatg acgatactga ccggcgaaga aggccttgtg gtgtatgatc cggtgctgaa
      661 aaaatggctt tcgtcccagt tcttccccta cggcgcgctg aaaaaggcgc aggcgggcga
      721 cgtgcttgcg gcagcttggt cggacaacga cctcgtgctc tacgacatga cgatccatga
      781 ttgggtggtc aaggagggga tttcgcctca ggcatgcatt atcgacgggt acaaggcgtc
      841 catcttcacc gcggacacgg tgtacaccta cgacgcggtc agccacagat ggagcagcaa
      901 gaacagatga gaagcggcgc gagacggcgg agaggaggcg ctccgacaat gaacggcagg
      961 cgaaacatat cggctttctc ggtgctgatg ctgcttacac tgctcgcact gccggggagg
     1021 gctttcgcgg cttcaatgat cgtcgccgtg cagcctttcc tcgcccaggg aacgagcttt
     1081 cacctcggcg cgacggtcgg cgatatcgtc tccacttcgc tcgcagggaa ccgtgacatc
     1141 acgctcgttg aacgatcccg actggccgag gtcgcccgtc agcagcggct cgccatgtcc
     1201 ggtgtcgtgg aaaccgccac ggcggcgaag gcggggaagc tcgtcggcgc gcgctacttc
     1261 atcctcggcg cggtcagcag attcggggcc ctgctcgtgg ccacggcgcg gcttgtcgac
     1321 gtggagagcg gtcgtgtgct cgcgagcttc gaacgaacct cgaaacaggg agaggacgaa
     1381 acgacgctcg tatcgcgaaa cctggcctcc gacattctgg ccttcatcag cggcgacgcc
     1441 cccgcgcccg gagatccgac gagcgactac aagtactatc tctacgaggc gttggggtac
     1501 tataatatgg gagagtaccg caagtcgctt ccgttctggg agaaaatgac gcagctcagc
     1561 ccgagaaacg gacttctgcg tttcatcgtc gcggggctcc acttccagac gaggcgttat
     1621 tccgacgcgc tgctcgcggc ccagcaagca gtcacatggg agccatc
//
LOCUS       DAOSWW010000678         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_63596_length_1663_cov_3.172886, whole genome shotgun sequence.
ACCESSION   DAOSWW010000678 DAOSWW010000000
VERSION     DAOSWW010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63596_length_1663_cov_3.172886"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..280)
                     /locus_tag="PK442_11885"
     CDS             complement(98..280)
                     /locus_tag="PK442_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88394.1"
                     /translation="MQLWAAALLFVLFAAAAFSFLKGGRNKSGIRFYTGILFAFIGGV
                     ALAYIAATVFFVVSIE"
     gene            complement(295..765)
                     /locus_tag="PK442_11890"
     CDS             complement(295..765)
                     /locus_tag="PK442_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88395.1"
                     /translation="MKKTLVKTAGLFVLAALLAVHCASRAEAWIKITLKNNRSHNIAV
                     AFCWSGFDYADDQRKGWYVVQPNDSRVINLANATSSMTWEDFGFYAQGTLRNGKKIRW
                     SGDMKQVIIHPSKAFDGHPEDPIAGGKKVGFRKLNLKKVGDANTDAVATLTFNP"
     gene            complement(931..1218)
                     /locus_tag="PK442_11895"
     CDS             complement(931..1218)
                     /locus_tag="PK442_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88396.1"
                     /translation="MRVQTGYIRSVKALPGYKLEVEMTTDTRIEFDFASRLNTIRFGA
                     LKDEKLFVTASTDGNFITFGTNDAVKVRISAIDFMDLVLVDRTGRFPPDET"
     gene            complement(1336..>1663)
                     /locus_tag="PK442_11900"
     CDS             complement(1336..>1663)
                     /locus_tag="PK442_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO88397.1"
                     /translation="PFAELGTTLLPLLENAKAGFNMKQMNALIALCKRHSSGAASVRR
                     HLDPSTALLTPREREIALLAKERCSTREIASRLFISENTVKSALKIIYGKLEVHSKNE
                     LATMDF"
BASE COUNT          355 a          443 c          425 g          440 t
ORIGIN      
        1 acacgtgaac cacctctttc tttgaatgtg ttcgcatttc gtttcgtttc aacatgaatg
       61 cgaaggcatg cctgcgactg ccgtttcccg tactcgctta ctctatcgaa actacgaaga
      121 agactgtcgc cgcgatgtac gccaatgcaa caccgccgat gaacgcgaag agaatgcctg
      181 tgtagaagcg tatcccgctt ttgttccttc cccctttgag gaaagagaac gccgccgccg
      241 cgaaaaggac gaacagaagc gccgctgccc agagctgcat accttgtgtc gccgtcaggg
      301 attaaacgtc agggttgcta ccgcgtccgt atttgcgtct cccacttttt tcaaattgag
      361 tttgcggaag cccaccttct tgccacccgc gatagggtct tcaggatgtc cgtcgaacgc
      421 tttactgggg tggataatca cctgtttcat gtcgcctgac catctgatct ttttcccgtt
      481 tctcagtgtt ccctgcgcat agaaaccgaa gtcctcccat gtcatcgaag aagtggcatt
      541 tgccagattg ataactcttg aatcgttggg ttggacaacg taccagcctt ttctctgatc
      601 atccgcataa tcgaatccgc tccagcagaa ggcgaccgct atgttgtgcg atctgttgtt
      661 tttcagggtt attttgatcc aggcttctgc tctggaggcg cagtgtaccg ccaagagcgc
      721 cgcaaggacg aaaagccccg ctgtttttac cagtgtcttt ttcatcgtat tcctcccttt
      781 tcttacccgc ttgaccgggt atcgacgaac agcatatatt ccggcgtcct ccgaataccg
      841 gaaaagaaaa aaaggcgcag tgctcaagat ttccctgtga gcagttttgc ttggtgcaca
      901 gtcgtaacga gaacgaggtg aatcaagaag ctacgtttca tccgggggga acctgcccgt
      961 tctgtccacg aggacaaggt ccatgaagtc gatcgccgaa atcctgacct tcaccgcatc
     1021 attcgtcccg aacgtgatga agtttccgtc cgtggaggcc gtgacgaaga gtttttcgtc
     1081 tttcagcgcg ccgaaacgta ttgtattaag cctggaggcg aaatcgaatt caatacgggt
     1141 gtccgttgtc atctcaactt cgagcttgta ccccggcagg gctttgacgc ttctgatata
     1201 tcctgtttgt accctcattt tctcacaccc ctgtagaaga gcctttcccg cagagtccat
     1261 aagtattttg aaggaaaact gcttgttcta caccccctct ttcggggggg aacggggggt
     1321 ggtgttctac gctcgttaga agtccatcgt ggcaagctcg ttctttgagt gaacttccag
     1381 tttgccgtaa atgattttga gcgccgattt gacagtgttc tccgaaatga acagacggga
     1441 tgcaatttcg cgggtgctgc agcgctcttt tgcaagcagg gcgatctctc gttcccgcgg
     1501 cgtcagaaga gctgtggagg gatcaaggtg tcttcgtacg gatgcggcgc cggaagaatg
     1561 cctcttgcag agcgcgatca gggcgttcat ttgcttcata ttgaagccgg ctttcgcgtt
     1621 ctccagaaga ggcagaagcg tcgtcccgag ctccgcgaac ggt
//
LOCUS       DAOSWW010000679         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_63823_length_1660_cov_2.353894, whole genome shotgun sequence.
ACCESSION   DAOSWW010000679 DAOSWW010000000
VERSION     DAOSWW010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63823_length_1660_cov_2.353894"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..726)
                     /locus_tag="PK442_11905"
     CDS             complement(334..726)
                     /locus_tag="PK442_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88398.1"
                     /translation="MQWEKTTGRKMHCCEGTINGVPARFTVHTGRIETGRSRMFIGYF
                     ASVEIDGEPVTGADSSGIVFALRNCAEKLRARGVVLDAPALSERFYESGLSENSGWGY
                     MRDGDDEPVHYIDRTKKYTRPPRYDSKS"
     gene            complement(734..1429)
                     /locus_tag="PK442_11910"
     CDS             complement(734..1429)
                     /locus_tag="PK442_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88399.1"
                     /translation="MLTFGAHPKGGLVFTGNGVHVTSPAPQDAMEKKERENELFIANY
                     RLLWENRRRIFATPELYDAKLSFPALSCAYFGGGPLSLGILLELWDIGYWTVPCPECG
                     GTCRVTYWCGSPLSGMNFWWGFCLDCGVRIHKNIYKRSDEVNNGRGFSRESHRALELI
                     KKHSKPIVVKDPPVPSDFRTWSKKCRERYESGAVIPVKEPEKEIIPVDDTKSLEEVIE
                     LLSRSAADSAEGS"
     gene            complement(1436..>1660)
                     /locus_tag="PK442_11915"
     CDS             complement(1436..>1660)
                     /locus_tag="PK442_11915"
                     /inference="COORDINATES: protein motif:HMM:NF025021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO88400.1"
                     /translation="VQNASSPEVIRLLLDAGTFVNRVDSFGETALHAALAALARHNDA
                     KDIVRMITEAGGETVFPVEGKRFDSNSERR"
BASE COUNT          282 a          505 c          480 g          393 t
ORIGIN      
        1 tccccgttct ggacagcttg aaccacgcgg cgatctgcaa ggcaagaatc agtttgtcac
       61 gaaacgccat tccgtttccc ggaacctcta ttgatcggat aagcgcgctt ccggcactca
      121 tcggctattc ctccctcgcc gccgccccgc gcggtaccct gcggagtcgg cgtcctctcg
      181 ttgcgttcat ttctcttcaa gcgatactat accatggaaa ggcgtgcttg taaatagcac
      241 gtcgtgcttt ttattttccg gctctgcttt taattggagg attcttgaag gagggatccg
      301 aagggtcgcg tttgttcttc cgggaaggtc tgcttacgac ttggaatcgt accggggagg
      361 gcgtgtgtac tttttcgtgc ggtcgatgta gtggacgggc tcgtcatcgc cgtccctcat
      421 gtacccccac cccgaattct cggagagtcc ggattcgtaa aaccgttcgg agagcgccgg
      481 ggcgtccagc acgacgcccc gcgcccgcag tttttctgcg cagttcctga gcgcgaagac
      541 aatccccgag gagtcggcgc ccgtcaccgg ttcgccgtct atctctacgg aggcgaaata
      601 gccgatgaac atgcgggaac ggcccgtttc gattcgcccc gtgtgcacgg tgaaccgcgc
      661 cggaacgccg tttatcgtac cctcgcagca atgcatcttt cgtccggtgg ttttttccca
      721 ctgcattctt ctctcagctc ccttccgcgg agtccgcggc gctgcgcgaa agcagctcga
      781 tcacttcttc cagcgatttc gtgtcgtcga ccgggatgat ttctttttcc ggttccttga
      841 cggggatgac ggcgccgctc tcgtaccgtt cacggcactt tttcgaccat gttctgaagt
      901 cggagggcac gggcgggtct ttcacgacga tgggtttgct gtgttttttt atcagctcca
      961 gcgccctgtg cgactcgcgg ctgaatcccc tgccgttatt cacctcgtcc gatcgtttgt
     1021 agatgttctt gtgtattctg acgccgcagt cgaggcagaa gccccaccag aagttcatgc
     1081 cgctcagcgg actgccgcac cagtacgtca cgcggcaggt tccgccgcat tcggggcagg
     1141 gcacggtcca gtagccgatg tcccacagtt ccagcaggat gccgagggag agggggccgc
     1201 cgccgaaata agcgcaggag agcgcgggaa aggagagctt ggcgtcgtac agttcgggtg
     1261 tggcgaagat gcgcctcctg ttctcccaaa gaagccggta gttggctatg aacagctcgt
     1321 tctcgcgctc ttttttctcc atcgcgtcct gaggcgcggg agaggttacg tgaacgccgt
     1381 ttcctgtgaa cacaagtccg cccttggggt gcgcgccgaa tgtaagcatg ggcaatcatc
     1441 tcctctcaga gttcgaatcg aagcgttttc cttcgaccgg aaagaccgtt tctccgcccg
     1501 cttccgttat catccgcacg atatccttcg cgtcgttgtg ccgcgcgagc gccgcgagcg
     1561 ccgcatggag cgccgtctct ccgaacgagt cgacccggtt cacgaaggtc cccgcgtcga
     1621 gcagcagccg gatcacttcg ggggaggacg cattttgaac
//
LOCUS       DAOSWW010000680         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_63859_length_1659_cov_3.955112, whole genome shotgun sequence.
ACCESSION   DAOSWW010000680 DAOSWW010000000
VERSION     DAOSWW010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63859_length_1659_cov_3.955112"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK442_11920"
     CDS             complement(<1..198)
                     /locus_tag="PK442_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOO88401.1"
                     /translation="MENGTRLNRFLAMCGIGARRKVEEIIASGRVRINGKVVLLPGYR
                     VEPDMDVSVDGQTVRPQEHRYL"
     gene            complement(248..856)
                     /gene="scpB"
                     /locus_tag="PK442_11925"
     CDS             complement(248..856)
                     /gene="scpB"
                     /locus_tag="PK442_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOO88402.1"
                     /translation="MTSSLPRFETLSSTAKIIEAILFAAPSPVSVNEMATLLSLSPSA
                     VKSELEDLRSYLLRHGHGITVIESAGGWRMATAEDVSEIVGAFRETAIAQKIRLSKAA
                     VETLAVIAYNQPVTRSEIEELRGVRCERVIETLLSHALIRIAGRRKATGSPLLYRTTD
                     RFLETFGVLSISELPTLEELDELAPVDEPHFVPVDEEERTNG"
     gene            complement(831..1604)
                     /locus_tag="PK442_11930"
     CDS             complement(831..1604)
                     /locus_tag="PK442_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOO88403.1"
                     /translation="MEERLGELAEFQAEIDGFSGPFDLLCWLVESRELEAASISVGQV
                     VRIYGAYLANTNKVSVSVVSEFLFMAASLVLNKIRALLPGRESLYESEEPENDPGEDV
                     LEKLSRYRPYRSAARDLAILKEERDRFFFRSAPEEEDDEPSFDLGDLFALCRLWWSLR
                     DLKRRTKNAAPYIFENEDDWSGVPSSLPEEEQIERKISEILSELNSDGSLRLSFLLRE
                     NRAMANFVVTLLALLEMSRMGRIRILQEELFDDIVITPL"
BASE COUNT          391 a          463 c          421 g          384 t
ORIGIN      
        1 aaggtatcgg tgttcttgcg gccggacagt ctgaccgtca acgcttacat ccatatcggg
       61 ctcgacgcga taccctggaa gaaggacgac cttgccgttg atacggacgc gtccggaggc
      121 gatgatttcc tccactttcc gtcgggcgcc tataccgcac atcgcaagga atctgttcag
      181 tcgcgtcccg ttttccacgg cgtactcctc ctccgctcaa agagctttat aaaatcttca
      241 ggggaaatca cccgttcgtg cgttcctcct cgtccacggg aacaaaatgc ggttcgtcaa
      301 ccggcgcaag ttcgtcgagt tcctccaaag tgggaagttc cgagattgaa agaacgccga
      361 atgtttctaa aaaacgatcc gttgtgcgat acaggagggg cgaaccagtc gcctttcttc
      421 gtcccgctat ccggataagc gcgtgcgaga gaagcgtttc gatcactctt tcgcaccgaa
      481 cacctcttag ttcctcgatc tcgctgcgcg ttaccggttg attatatgcg atgaccgcca
      541 atgtctccac tgccgctttg ctgagacgga tcttctgtgc gatcgctgtc tcgcggaagg
      601 ctcccacaat ttccgataca tcctccgccg tcgccatccg ccaccctcct gcggactcga
      661 ttacggtaat gccgtgtcca tggcgcagga gatacgaacg aagatcctcc agctctgatt
      721 ttactgcgga aggagaaagc gaaagcagcg tagccatctc atttacagaa acgggagaag
      781 gcgccgcgaa gaggattgct tcgattatct tcgcagtact tgaaagcgtc tcaaagcggg
      841 gtaatgacga tgtcatcgaa aagctcctcc tgcagtatcc ggattcgtcc catcctgctc
      901 atttcaagga gagcgagaag agtcacaaca aaattcgcca tcgctcggtt ttcgcggaga
      961 aggaatgaga gacgaagaga accgtctgag ttcagttcag aaagaatctc tgaaatcttg
     1021 cgttcaattt gttcctcttc cggtagggac gacggaacgc cgctccaatc gtcctcattc
     1081 tcgaaaatgt atggcgctgc attttttgtc cgtcgtttta aatctctcaa gctccaccaa
     1141 agacggcata gagcaaacag gtcgccaagg tcaaaagacg gttcatcgtc ctcttcctcg
     1201 ggggcgctgc ggaagaaaaa acggtcgcgt tcctctttga gaattgcaag atcccgtgca
     1261 gccgaccgat agggacgata cctcgatagt ttttcgagga catcttcacc gggatcgttc
     1321 tcgggttctt cgctttcgta cagtgattcg cgtcccggca gaagggcgcg aatcttgttg
     1381 agaacaagag aagctgccat aaacaaaaac tccgaaacta cggaaacgga gaccttgttc
     1441 gtgttggcca ggtaggcgcc gtaaatgcgg acgacctgac cgacggagat actggcggct
     1501 tccagctctc tgctttcaac gagccagcag agtaaatcaa agggaccgga aaaaccgtcg
     1561 atctccgcct ggaactctgc gagttctccc aggcgctcct ccactcctag cctctcttga
     1621 aaaagccgat tgcgttgatc acttcgtcaa tcgtacggc
//
LOCUS       DAOSWW010000681         1658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_63934_length_1658_cov_3.472863, whole genome shotgun sequence.
ACCESSION   DAOSWW010000681 DAOSWW010000000
VERSION     DAOSWW010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63934_length_1658_cov_3.472863"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1621
                     /locus_tag="PK442_11935"
     CDS             143..1621
                     /locus_tag="PK442_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO88404.1"
                     /translation="MRTVRRKSLFLSVFIPIVFILSHVPSEAAVRPLKQSYAPDEPIE
                     IVYDNLPGSQTDWISLAPRENADDRYGEWFYTGGMRSGSHTFGGLPAGVYEARVYHDW
                     PAGGYVVQERAAVFVQEAVPEQSGPVPAEIPAAMRPTPRPEPHPSPVPEPVEISWSAS
                     GQGTLFAPGQGVHNYRIHELDARRFLNGGTLIVDVRIGAGQSWASVDLYGDGQSLATS
                     GVPQALASAWDLPPNSNRRITHTFRKGQIFRLCFEGNWGSPQGATNTYTYSATIKPAP
                     EHEPQLPPLPERETPHVAPPENAVNRRAAETLWRQAVSFQQQKRYEEALAKYREGLAL
                     SEDPAVAHHADAMETCLLRLSTLPDIAATRPEAAEIDIFNNWNKTAVVNNPAGPPSPT
                     RFTISRPHAITYINTYHYFNNGTPAGSITLVHQDGTLYGPWPATGRSGQGGVQNAIWE
                     ATPQTTLKAGTYTVFDSHPSTWSHNAQSDLRGFALVKGRLLQ"
BASE COUNT          333 a          568 c          473 g          284 t
ORIGIN      
        1 aagactgctc gaagagcgtt ccgacgcccg cccacgtccg cggacctttc tccaaaaatg
       61 aaaacgctcc tcttttgcga aaaaagccct gcttctggta tcatggggtc actattcaca
      121 cgagcgaggg ggagtgcact ctatgcgtac cgtacgacga aagtctcttt ttctctccgt
      181 tttcatacct atagtattca ttctgtcaca cgtgccttcc gaggccgccg tccgcccgct
      241 caagcagtcc tacgcgccgg acgaacctat cgagatcgtc tacgacaacc ttcccggaag
      301 ccagaccgac tggatttcgc ttgctccgcg ggagaatgcc gacgaccggt acggcgagtg
      361 gttctacacc ggcggcatgc gctccgggag ccatactttc ggcggattgc ccgcaggagt
      421 ctacgaggcg agagtctacc atgactggcc ggcgggcggg tacgttgtac aggaacgcgc
      481 cgccgtcttc gttcaggaag ccgttccgga gcagtccggt ccggttccgg ccgagattcc
      541 agcggcaatg cgtccgactc ctcgcccgga gccccatccc tcccccgtcc ccgaacccgt
      601 cgagatctca tggtccgcgt cgggacaggg aacgctcttc gctcccggac agggcgtcca
      661 taactatcgc atccacgaac tggacgccag acgcttcctc aacggcggta cgctgatcgt
      721 cgacgtccgc atcggcgcgg ggcagagctg ggcgtcggtc gacctgtacg gggacggtca
      781 gtcactcgcg acctctggag tgccgcaggc ccttgcgagc gcatgggatc tgcctccgaa
      841 ctcgaaccgg cgcataacgc atactttccg gaaagggcaa atctttcgtc tctgcttcga
      901 ggggaactgg ggatcgccgc agggcgccac caacacctat acgtacagcg cgaccatcaa
      961 gcccgctccc gagcacgagc cgcaacttcc tcccctcccc gagcgtgaaa caccgcacgt
     1021 cgctccgcca gaaaacgcgg taaaccggcg cgctgcggag acgctctggc ggcaggccgt
     1081 ctccttccag caacagaaac ggtacgaaga ggcgctggcg aaataccggg aagggctcgc
     1141 tctctccgag gatccggcgg tcgcacacca tgccgacgcg atggagacat gcctcctgcg
     1201 gctgagcact cttccggaca tcgccgcaac ccgtccggaa gcggcagaaa ttgatatctt
     1261 caacaactgg aacaaaaccg ccgtggtgaa caaccccgcc gggccgccct cgccgacgcg
     1321 cttcacgatc tcccggccgc acgcgatcac ctacatcaat acgtaccact acttcaacaa
     1381 cggaactccg gccggaagca tcacgctcgt ccaccaggac ggcacgctct acggaccgtg
     1441 gcccgcgacg ggccgctccg ggcagggagg cgtacagaac gcgatatggg aggcgacccc
     1501 gcagacgacg ctgaaagccg ggacgtacac cgtcttcgac tcgcacccgt cgacgtggtc
     1561 ccacaacgcg cagtccgatc tgcgcggctt cgcgctcgta aaaggccgcc tgcttcagta
     1621 gattctaccg gtgtatgctc cgttggtatg agaaggag
//
LOCUS       DAOSWW010000682         1658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_63937_length_1658_cov_3.106675, whole genome shotgun sequence.
ACCESSION   DAOSWW010000682 DAOSWW010000000
VERSION     DAOSWW010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63937_length_1658_cov_3.106675"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..928
                     /gene="metF"
                     /locus_tag="PK442_11940"
     CDS             38..928
                     /gene="metF"
                     /locus_tag="PK442_11940"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOO88405.1"
                     /translation="MLIRDLLSRTRPAISFEIFPPKPETPIETVFKTVAGLKELSPDF
                     ISVTYGAGGSSRDRTVEIASAVHNRWGLVSLAHLTCMGSSPAEVEKVMEDLEQEGVCN
                     ILAMRGDPRPDGGENHFQHASDLIHHIRRGHFRDFSLGAAAYPEGHPESPSLEEDLLH
                     LKEKVDAGADFLITQLFFDNEIFFRFMDRIRALGIAVPVLAGIMPVLNARQIGRIVSL
                     CGASIPPRFARIMSRYEHSPEALSEAGIAYATEQIIDLLSWGADGIHLYTMNRPDVAK
                     RIMESIATVRRTLNGSSSGV"
     gene            complement(1080..>1658)
                     /locus_tag="PK442_11945"
     CDS             complement(1080..>1658)
                     /locus_tag="PK442_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO88406.1"
                     /translation="TVMWTLAGVVERSGGSVDIFGESLPTKPHEIVARGLALVPERRR
                     LFSALTVSENLIMGAYRRKADASVEEDMERCFQLFPILKKRLNQRSGTLSGGEQQMLA
                     ISRALMSKPRILLLDEPSLGLAPLLVETLMETILRIRSEGMTVLLVEQNATQALEISD
                     RGFILEAGRFQKSGPGKELAGDPEIRKAYLGG"
BASE COUNT          349 a          517 c          458 g          334 t
ORIGIN      
        1 gccgaaaaaa tcagtaaaaa ggaggtatgc acgcgctatg cttatccgtg atcttctctc
       61 gcgaacgcgt ccggcgatct ccttcgagat atttcctccg aaaccggaaa cgcctataga
      121 gacggtattc aaaaccgtgg ccgggctgaa agagctctct cccgatttca tcagcgttac
      181 gtacggcgcc ggaggaagca gccgcgacag gaccgtggag atagcctccg cggtgcataa
      241 ccggtggggg cttgtttctc ttgcgcatct cacctgcatg ggttcttccc cggcggaggt
      301 tgaaaaagta atggaggatc tggagcagga aggtgtctgc aatatactcg caatgcgggg
      361 agatccccgc ccggatggcg gagagaacca ctttcagcac gcatccgacc ttatccatca
      421 tatccggcgc ggacattttc gagacttctc cctcggcgct gcggcctacc ccgaggggca
      481 cccggaatcg ccttcacttg aggaggatct cctccacctg aaggagaaag tcgatgcggg
      541 agcggacttt ctgatcacgc agctcttctt cgacaatgaa atcttcttcc gtttcatgga
      601 cagaatacgc gccctgggaa tcgctgttcc ggtgctggca ggaatcatgc cggtgctcaa
      661 cgcacggcag atcgggagga tcgtctccct ctgcggcgcg tcgatcccgc cgcgttttgc
      721 ccgcatcatg tcccgctacg agcactctcc cgaagctctt tcagaggcgg ggatcgccta
      781 cgcgacggag cagatcatcg atcttctctc ctggggcgcc gacggtatcc acctttacac
      841 gatgaaccgc cccgacgtgg cgaagcgcat catggagagc atcgccacgg tgcgaagaac
      901 gctcaacggg agttcctccg gagtctgacg aatcctgaga gagtacaaaa caatgctgag
      961 ggatgatccg aggcttaccg cctcctgaat caatccctca gcattcaata ccagggttct
     1021 gcccgaaaca gacacctgca atcccgcatt ttttccgcag tcgtcggagt ccctcaggtt
     1081 tagccgccga gataggcctt ccggatctcc gggtctcctg cgagctcttt gccaggcccg
     1141 cttttctgga aacgccccgc ctccaggata aaccctcggt cgctgatctc aagagcctgg
     1201 gtcgcgttct gctccaccag caggacggtc attccctcgc tacgaatcct gagaatagtc
     1261 tccatcagcg tctccacgag aagaggggcg agaccgaggg acggttcgtc cagaaggaga
     1321 attctcggct tgctcatcaa ggctctcgag atggcgagca tctgctgctc tccccccgaa
     1381 agcgttcccg agcgctgatt cagccgtttc ttcaggatcg ggaagagctg gaagcagcgc
     1441 tccatatcct cttcgacgct cgcatcggcc ttccgccggt acgcccccat gatcagattc
     1501 tccgacacag taagcgccga aaagagacgg cgacgttccg ggacaagcgc aaggcctcgc
     1561 gcgacgatct catgcggctt cgtcgggagg gattcaccga agatgtccac cgaaccgccg
     1621 gagcgctcca caactccggc cagcgtccac atgacggt
//
LOCUS       DAOSWW010000683         1653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_64268_length_1653_cov_2.792866, whole genome shotgun sequence.
ACCESSION   DAOSWW010000683 DAOSWW010000000
VERSION     DAOSWW010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64268_length_1653_cov_2.792866"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..>1653
                     /locus_tag="PK442_11950"
     CDS             286..>1653
                     /locus_tag="PK442_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOO88407.1"
                     /translation="MKRNWKTMDGNTAAAHVSYAFTEVAAIYPITPSSNMPEVVDEWA
                     AHGRKNIFGKTVRVTELQSEAGAAGAVHGSLSAGSLSTTFTASQGLLLMMPNMYKIAG
                     ELLPGVFDVTARSVAAHALSIFGDHSDVMACRGTGFAFLASGSVQEVMDLTAVSHLSA
                     IKGSLPFVNFFDGFRTSHEIQKIDVLEYDDLAKLVDYDALAKFKARAMNPEHPYQKGT
                     AQNPDIFFQAKEAVNRFYTVIPDIVESYMQEIKKLTGREYHPFNYYGDPNAENIIIAM
                     GSVCQAIEETVDYLNARGEKTGVVEVHLYRPFSEKYFFDVLPASVKRIAVLDRCKEPG
                     ALGEPLFEDICAIFKDKAEKPIVVGGRYGLGSKDTTPTHIKSVFDNLKLYQPRDRFTI
                     SIVDDVTGTSLPVNEQIVAAPEGTIRCKFWGLGSDGTVGANKNSIKIIGDETDLYAQG
                     YFAYDS"
BASE COUNT          421 a          438 c          412 g          382 t
ORIGIN      
        1 aaggtttatg gtaatatcaa tacgtgaaaa aaatactttt tggttgtcgt ttttttccag
       61 aagtgcggat tatacagaat gcaaaatccc gatcttgttg taaaatagaa gggtgttaac
      121 cggaagtcga attcccatct gaccttcggc gtatttgtgc ggacattaca agagcccgct
      181 ggaaagatca caatggggct tattgcgtct atggatcaag aataataaaa caggagcggg
      241 ttgcgacaac gctgcccctc ctgtaattca agagggggta catcaatgaa gagaaattgg
      301 aaaacgatgg acggcaacac tgctgcagcg catgtttcat atgcgttcac cgaggttgct
      361 gcaatttacc cgattacgcc gtcctccaat atgcccgaag ttgtcgatga atgggctgcc
      421 cacggcagga agaatatttt cggcaagact gtcagggtca cggaacttca gtctgaagcg
      481 ggagccgcag gcgccgtgca cggttctctt tcagccggat cgctttcgac caccttcaca
      541 gcatctcagg gtttgttgct tatgatgccg aatatgtaca aaatcgccgg cgaactactt
      601 cccggtgtct ttgacgtgac tgcccgctca gtcgccgcgc atgccctttc catcttcggc
      661 gaccacagcg acgtcatggc ttgccgcgga acgggttttg catttctcgc atccggcagc
      721 gtccaggaag taatggatct gaccgctgtc tcccacctct ccgcaatcaa ggggagtctt
      781 ccttttgtca acttcttcga cggcttcaga acctctcacg aaattcagaa gatcgacgtg
      841 cttgagtacg acgatcttgc caaacttgtc gactatgacg cgcttgcaaa attcaaggcg
      901 cgcgcaatga atccggaaca cccctaccag aagggaaccg cgcagaaccc cgacatcttc
      961 ttccaggcga aggaagcggt caacagattc tatactgtta tcccggacat cgtcgagtcg
     1021 tacatgcagg agatcaagaa gctcacaggc agagagtatc acccgttcaa ctactacggc
     1081 gacccgaacg ctgaaaacat catcatcgca atgggttctg tctgtcaggc gattgaggaa
     1141 acggtcgact acctcaacgc gcgcggcgag aagacaggcg ttgtagaagt gcatctgtat
     1201 cgccccttct ccgagaaata cttcttcgac gtgcttcccg catccgtaaa gcgcattgct
     1261 gtgctcgaca gatgcaagga gcccggcgct ctcggcgagc ccctctttga ggatatttgc
     1321 gctattttca aggacaaagc ggaaaaaccg atcgttgtcg gcggacgcta cggcctcggt
     1381 tccaaggaca ccacaccgac acatattaaa tctgtcttcg acaatctgaa gctttatcag
     1441 cccagggatc gtttcaccat cagcatcgtc gacgacgtta ccggcacgtc actgccagta
     1501 aacgagcaaa ttgtcgcagc tcctgaagga acaatcagat gcaaattctg gggccttggg
     1561 tccgatggaa ctgtgggcgc caacaagaac tcgattaaaa tcatcggcga cgaaacagac
     1621 ctttacgcac aagggtattt tgcgtacgat tcc
//
LOCUS       DAOSWW010000684         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_64303_length_1652_cov_4.063244, whole genome shotgun sequence.
ACCESSION   DAOSWW010000684 DAOSWW010000000
VERSION     DAOSWW010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64303_length_1652_cov_4.063244"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PK442_11955"
     CDS             <1..309
                     /locus_tag="PK442_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO88408.1"
                     /translation="GQTVTVHGPEAKLADGTLAGSVLTLDLALRNFAAATGLPLWEAT
                     RLVSENPARLLGERDRGRIEPGYRADFVLLDEGWRVLKTFVEGKEVFDRHENRDCKGL
                     "
     gene            281..1018
                     /gene="nagB"
                     /locus_tag="PK442_11960"
     CDS             281..1018
                     /gene="nagB"
                     /locus_tag="PK442_11960"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOO88409.1"
                     /translation="MRIVTAKDYDQMSRKASYIVASRVILNPKCVLGLATGDTPKGLY
                     NELVRLFRAGDVDFSGVTTFNLDEYAGLSPENPQSYHSYMEEHLFRHINIPPEHRHIP
                     NGAAQNIGAECRRYEELIEQAHGIDLQILGLGRDGHIGFNEPDVKFEKGTHHVRLTQS
                     TIEANARFFENRDEVPKSAISMGIKTIMSARRILLLASGPEKAEAIRGAVMGAVTPDL
                     PASVLQLHPNATLIVDEAASAEIMRIK"
     gene            1056..>1652
                     /locus_tag="PK442_11965"
     CDS             1056..>1652
                     /locus_tag="PK442_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88410.1"
                     /translation="MVRRILDANASDLAKMTGRELLTSIRAAEGRTLVAETIAPAPPL
                     LGDVTNAELARAFGADIILLNMFDVRAPHIEGLDVLNDDDFFLRTYGQGHRVRATETT
                     RAQTGTIAALKTLIGRPIAINLEPVDEGERVLGPQAKLPEGRRATPENARLAVKQGAD
                     MLVVTGNPATGVTTASIAASLRGIREAVGPDPILAAGKM"
BASE COUNT          330 a          555 c          534 g          233 t
ORIGIN      
        1 ggccagacgg tcaccgtcca cggcccggag gcgaagcttg ccgacggaac gctcgccgga
       61 agcgtcctca cgctcgatct cgcgctgcgg aactttgcgg ccgcaaccgg cctccccctc
      121 tgggaggcga cgcgcctcgt ctcggagaac cccgcccgat tgctgggcga gcgggacagg
      181 gggcgcatcg agccggggta ccgggccgac ttcgtcctcc tcgacgaagg gtggcgcgtc
      241 ctcaagacat tcgtggaagg aaaggaggtc ttcgaccgcc atgagaatcg tgactgcaaa
      301 ggattatgac cagatgagcc ggaaggcgtc gtacatcgtc gccagccgtg tcatcctgaa
      361 cccgaagtgc gtgctcgggc tcgccacggg ggacaccccc aaggggctct ataacgaact
      421 ggtccgcctc ttccgggcag gggacgtcga cttctccggc gtcaccacgt tcaacctgga
      481 cgaatacgcg gggctctcgc cggagaaccc gcagagctac cacagctaca tggaggaaca
      541 tcttttccgg cacatcaata tcccgccgga gcaccggcat attcccaacg gcgcggcgca
      601 gaacatcggc gcggagtgcc gccgctacga ggaactcatc gaacaggcgc acggcatcga
      661 cctccagatc ctgggactcg gtcgggacgg gcatatcggc ttcaacgagc cggacgtgaa
      721 gttcgagaag gggacgcacc acgtccgtct cacgcagagc accatagagg cgaacgcccg
      781 tttcttcgag aaccgcgacg aggtgcccaa gagcgccatc agcatgggga tcaagacgat
      841 catgagcgcg cggcggattt tgctgctcgc ctcgggaccg gagaaggcgg aggccatccg
      901 cggcgcggtc atgggggcgg tgacccccga cctgcccgcg tcggtgctcc agctgcaccc
      961 caacgcgacg ctcatcgtgg acgaagcggc aagcgccgaa atcatgcgca tcaagtgacc
     1021 ggcagaactg ctgtactcag gaaaggagca tcatcatggt acgacgaatt ctggacgcca
     1081 acgcctccga cctcgcgaag atgacggggc gggagctgct gacgagcatc cgcgcggcgg
     1141 agggacgcac gctcgtcgcc gagaccatcg cccccgcccc cccgctgctt ggcgacgtaa
     1201 cgaacgccga gctggcacgt gctttcggcg cggacatcat cctgctgaat atgttcgacg
     1261 tccgcgctcc gcacatcgaa gggctggacg tactgaacga cgatgatttt ttcctgcgca
     1321 cgtacggcca gggacatagg gtgcgggcga ccgaaacgac ccgtgctcag accggaacca
     1381 tcgcggcgct caaaacactt atcggccgcc ccatagcgat caacctcgaa ccggtcgacg
     1441 aaggagagcg cgtgctcggc ccgcaggcga agctccccga gggacggcgc gccactccgg
     1501 agaacgcacg cctcgccgtg aagcagggag cggacatgct cgtcgtcacg ggcaaccccg
     1561 cgacgggcgt caccacggcc tccatcgccg cgtcgctgcg gggaatccgc gaggccgtcg
     1621 ggcccgaccc gatcctcgcc gcggggaaaa tg
//
LOCUS       DAOSWW010000685         1651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_64379_length_1651_cov_3.181078, whole genome shotgun sequence.
ACCESSION   DAOSWW010000685 DAOSWW010000000
VERSION     DAOSWW010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64379_length_1651_cov_3.181078"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            480..1553
                     /locus_tag="PK442_11970"
     CDS             480..1553
                     /locus_tag="PK442_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88411.1"
                     /translation="MVAALCLSKKTSLDEWGSFEFGKAARAYSRARRFREWLRNPRID
                     PGFLLHPLTERLISKAGKHVHIALDTSILFNAFCMVRVSLIFLDRAVPLVWSVRKSKS
                     ASVPFSAYRGLLEEVAAMIPSGTQPIFLADRGFQNASLFELLKQLGWWCRIRAKSDAP
                     VRTRKGEAIIPGKLCLKRGLPRFWRNASYGGVGGVTFGALLPMAAGEEPWYIVSFGGD
                     FTDIFRDYSKRFCIEEEFLDEKSGCFGLSKSRIRDEKRIERLLCVIATAAVLMVELGI
                     ETERAGERANVDTHGKRGLSYLKIGLRKLMQILHFPKLLPIWIRLSPLPFDYEPEGVS
                     VSRKEDEETARRKRKCEVYEVYD"
BASE COUNT          356 a          457 c          472 g          366 t
ORIGIN      
        1 cgtagctgag aaagatacgg agaggtgtcg ccgagagggg tgagagaact gtcttcttct
       61 cctcgctcca ttcgtgaccg cactcttcgc agacatacac ttcgcgcttc ttgctgtaca
      121 tcggatctgc ggtgttgcac ttcgggcact tgatttccat tattgcatag ctcctttcga
      181 ttgcctatgg aacgtttgag tctgactgac ggaggaatct ttttcaattc accatgataa
      241 agtatggatt gaatgtcctc cgccccgtac cggcagcggt gcgaaacaat aaagcacctt
      301 gaaaaaagaa cggcctgtag acaattcatc gtttcccgtg ttcaatacgt tcgccgaaac
      361 tatacagaac gggggaatcg atcatcaaca ggccgcctct acatactaca atcatccgcc
      421 tcctttatca acacctcgct ccgtttctcg accgaaggct catagaaacg ctcggcgcga
      481 tggtggcagc tctctgcctg tcgaaaaaga cgagtctcga cgaatggggt tccttcgagt
      541 tcggcaaagc cgcgcgcgca tacagcaggg cgaggcgttt ccgcgaatgg ctccgcaatc
      601 cgaggatcga tcccgggttc cttcttcacc ctcttacgga gcgcctgata tcgaaagcgg
      661 gaaaacacgt ccacatcgct ctggacacgt ccatcctttt caacgcattc tgcatggttc
      721 gcgtctccct gatctttctc gacagggcgg ttccgctggt ctggtccgtg cggaagagca
      781 agagcgcctc ggttcccttc tccgcgtatc gggggctcct ggaggaggtc gcggccatga
      841 ttccgtcggg gacgcagccg atctttctcg cggacagggg attccagaac gcttctctct
      901 tcgagcttct gaaacagctc ggttggtggt gcagaataag agcgaagagc gacgctcccg
      961 tacggacgag gaagggggag gcgatcatac cggggaaact ctgtctgaag cgcggactcc
     1021 ccagattctg gagaaacgcc tcctacggcg gagtcggcgg cgtgacgttc ggtgcgctgc
     1081 tgccgatggc cgcaggcgaa gagccgtggt acatcgtctc cttcggagga gacttcacgg
     1141 acatcttccg cgactactcg aaacgcttct gcatcgaaga ggagtttctc gacgagaaga
     1201 gcgggtgttt cggtctttcg aagagccgga tacgcgacga gaagaggatc gagcggctgc
     1261 tgtgcgtgat cgcgacggcc gccgttctga tggtggaact cggaatcgag acggagcgcg
     1321 cgggagagcg cgcgaacgtc gatactcacg ggaagcgtgg tttgagctac ctcaagatcg
     1381 gattgagaaa gctgatgcag atactccatt tcccgaagct cctcccaata tggattcgac
     1441 tctccccgct tcctttcgac tacgagccgg aaggtgtttc ggtctcccgt aaagaggacg
     1501 aagagaccgc ccgccggaaa cgcaagtgcg aagtttacga ggtgtacgat tgacgatgcg
     1561 ctttattacg gtgaagagaa gaagattcct ccgtcagcca ggcgtcgacc ctatcgatca
     1621 cgttgcatcc tggattcccg gagacctcgt t
//
LOCUS       DAOSWW010000686         1648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_64576_length_1648_cov_2.967985, whole genome shotgun sequence.
ACCESSION   DAOSWW010000686 DAOSWW010000000
VERSION     DAOSWW010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64576_length_1648_cov_2.967985"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /gene="infB"
                     /locus_tag="PK442_11975"
     CDS             <1..1444
                     /gene="infB"
                     /locus_tag="PK442_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOO88412.1"
                     /translation="IRKTNITAREAGGITQHIGASIVMHEGRRIVFLDTPGHEAFTSM
                     RARGAQATDVAVLVVAADDGVMPQTREAINHAKAAGVPIVVAINKVDKPAAKADRVRQ
                     QLSDVGLAPEEWGGSTIMVEVSAKSGQGIPQLLEMILLVSEMEDLKADPTVRPEGVVV
                     EAKLDKGKGPVATVLVQQGSLCKGDIVLFDTAWGKIRAMIDAEGNPIDCAGPSTPVEI
                     LGLTAVPQPGEVFKAVENERIARDALSVKEQEVRNAQQSGPRKLTLEELYSQLKEGDI
                     PQLNLLLKCDVQGSSEALAASLEKLATNEVGINIVHRGVGRIAESDVMLASASNAVII
                     GFNVRPDANAKKVAESEGVQVRLYDIIYDAIDDVKAALEGMLKPTIRENTLGQAEIRQ
                     VIRVPKAGNVAGSYVLEGVIRRNAKARLVRNGIVLWDGSLANLRRFKDDAREVAAGYE
                     CGISLSNFQDFFFFKQKTAYEIIEEKRHLS"
BASE COUNT          382 a          438 c          506 g          322 t
ORIGIN      
        1 catccgcaag acgaacatca ccgcccggga ggcgggcggc ataacgcagc acatcggcgc
       61 ttccatcgtg atgcacgaag gaagacgaat cgtttttctg gatactcccg gacacgaggc
      121 gttcacctcg atgcgcgcac ggggagccca ggctaccgac gttgcagttc tcgtcgtagc
      181 agcggacgac ggagtaatgc cgcagacgcg cgaggcgata aaccatgcca aggccgccgg
      241 ggtgcccatc gttgtggcaa taaacaaagt ggacaagcct gcagctaaag cggacagagt
      301 tcggcagcag ctttccgatg ttggtctggc gcccgaggaa tggggcggct ccacgatcat
      361 ggttgaagtt tccgcgaaat cgggacaggg tattccgcag cttctggaga tgattcttct
      421 tgtctcggaa atggaagatc tgaaggcgga tccgacggtt cgccccgaag gcgttgtcgt
      481 ggaagcgaag ctcgacaagg ggaaaggacc cgttgcaacg gttcttgtgc agcaaggctc
      541 tctctgcaag ggagatatcg tgttgttcga taccgcgtgg ggcaagatca gggctatgat
      601 cgatgctgaa gggaacccga tcgactgtgc gggcccgagc acgccggtgg aaattctcgg
      661 ccttaccgcc gtaccgcagc cgggtgaagt cttcaaagcg gttgaaaacg aacgcatagc
      721 ccgcgacgct ctcagcgtaa aagagcagga agttcgtaat gcccagcaga gcggtccgag
      781 aaaactcacg ttggaggagc tctactcgca gctgaaggaa ggcgatattc cgcagctcaa
      841 tcttctgctg aagtgcgatg tccaggggtc gagcgaggcg cttgccgcat ctttggaaaa
      901 acttgcgacg aacgaagtcg ggattaacat agttcaccgc ggagtcggca gaatcgccga
      961 atccgacgtg atgctcgcct ccgcgtcgaa cgccgttatt attggtttta acgtgcgccc
     1021 ggacgcgaat gcgaagaaag tagccgagtc ggaaggtgtc caagtacgtt tgtacgacat
     1081 catctatgac gctatcgacg acgtgaaggc cgccctcgaa ggtatgctga agcctaccat
     1141 tcgggagaac acgctgggcc aggcggagat ccgccaagtc attcgcgtcc cgaaggccgg
     1201 taacgtcgcc ggaagctacg tgcttgaagg cgtaattcga aggaacgcga aagcccgcct
     1261 cgtgcgcaat ggtattgtgc tctgggacgg ctcccttgca aaccttcgcc gtttcaagga
     1321 cgatgcacgc gaggtcgccg cagggtacga atgcggcatc agcctctcca acttccagga
     1381 cttttttttt ttcaagcaga agacggcata cgagatcatc gaggagaaaa ggcatctctc
     1441 ctaatgaacc gaagggttcc cgttccgtac tcaggtttcc tgtttgttgc cctcgaaata
     1501 atgggaagca gaagcatgaa ggaccggaga caggtgacgc aatccctgct cgacaggatc
     1561 agacgacgtt ggaatgtttc agccatggat ctcgggcccg acggaagctg gtccgaggct
     1621 tttgtggctg tctccgcagc cgcttcag
//
LOCUS       DAOSWW010000687         1644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_64816_length_1644_cov_5.643801, whole genome shotgun sequence.
ACCESSION   DAOSWW010000687 DAOSWW010000000
VERSION     DAOSWW010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64816_length_1644_cov_5.643801"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PK442_11980"
     CDS             complement(<1..793)
                     /locus_tag="PK442_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HOO88413.1"
                     /translation="MMLSPLFVFSPGRALLALLAFALSSATCVYARRYFEGADERRFF
                     MYGSLGFLLSVWGTLFSAHWITFAVFMEISTAFLFSLIALKERDVALRYFVVQLVGAA
                     FLLAGIGAAYAGHGPALVGPVPKDALPFFVLGLGVKAALPVLHFWLPEVHGRAPAPVS
                     ALLSGMAVKIGAFGLLQALGADGPNFLAPVGAIMALWGVALALAQHDMKRLLAYHTVS
                     QLGYVIAAVGAGSASGWCGALYHMAAHGLFKSMLFLCAGVLEKAYC"
     gene            1180..>1644
                     /locus_tag="PK442_11985"
     CDS             1180..>1644
                     /locus_tag="PK442_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOO88414.1"
                     /translation="MKLRTKIVNALILLTFAVLVTAAPVMAADTVVIVDFSWDSVQMH
                     NRIAGYVIEKAFGKKVDYIFAESLPGMMGIERGDAHLSMEGWVDNLLEVWERMTSSGK
                     AESLGTNFPDAPQGWYVPTYMIKGDKERGIEPVTPDLKSVSDLAKYKKLFIDP"
BASE COUNT          446 a          462 c          419 g          317 t
ORIGIN      
        1 tgcagtacgc tttctccaga acgcccgcgc ataaaaagag catactcttg aaaagcccgt
       61 gagccgccat gtgatagagc gccccgcacc atccagaagc acttccggcg ccgaccgcag
      121 cgataacgta gcccaactga ctcaccgtat gataggccaa gaggcgtttc atatcatgct
      181 gcgcaagagc cagagcaacg ccccaaagcg ccattatcgc gccgacgggc gccagaaagt
      241 tcggaccgtc ggctccgagc gcctgaagca ggccgaatgc gcctatcttc accgccattc
      301 cggagagcaa cgcgctcacc ggcgcggggg ctcttccgtg aacctccgga agccagaaat
      361 gaagcacagg aagcgccgct ttcacgccta ggccgagaac aaaaaacggg agcgcatctt
      421 tcggaaccgg gccgacaaga gccggtccat gaccggcgta tgcggcgcct atacccgcca
      481 gaagaaaagc cgcccctaca agttgcacaa caaaataacg gagtgctacg tcacgctctt
      541 tcagcgcgat gagcgaaaaa agaaacgctg tggagatttc catgaagact gcgaacgtga
      601 tccagtgcgc ggaaaagagc gttccccaca ccgagagcag aaagccgaga gagccgtaca
      661 tgaagaaacg gcgttcgtcc gccccctcga aatatctcct ggcgtagacg caggttgcag
      721 agctcaaagc gaatgccagc aacgccagca acgctctgcc cggcgaaaaa acaaaaagcg
      781 gagaaagcat catgaggcca gttttcctga aaggaactcc gtacacatga cgacgtctcc
      841 gaacctgctc tgaaaggtca tcatctacgc atctctcctc tttcagcctt tcttgtttca
      901 atctttcttg agttcgtgtc gaatacccca acaaatcgga cgaaaacagt ataacaccat
      961 cgaaacaacg aacgaatgaa gaagaaccgc atacggagca actccagcca aaacagagtc
     1021 ttttgtatac tttttggata tttacatgtt gacaaatact caaacttgag tttaaatcag
     1081 atatcagctc cgaagcgccc gtcaaactta ccgctttttt ccgattcatc gcatttttct
     1141 actactcatg gtgaatcaaa tccaacagga ggtgtttgca tgaaactgcg aacaaaaatt
     1201 gtgaatgcgc taatcttgct cacgtttgcc gtcctcgtta cagccgcgcc ggttatggct
     1261 gccgatactg tcgtcatcgt cgacttcagc tgggacagcg tccaaatgca caatcgcatc
     1321 gccggttatg tcatcgagaa agccttcggg aagaaagttg actacatctt tgcagagtct
     1381 cttccgggga tgatggggat tgaacgcggc gacgcgcacc tctccatgga ggggtgggtc
     1441 gacaacctcc tcgaagtatg ggagcgaatg acgtcgagcg gcaaggcgga gagtctgggg
     1501 acgaacttcc ccgacgcccc gcagggatgg tacgttccta cgtacatgat caagggggac
     1561 aaggaacgcg gtatcgagcc ggtgacgccg gatctgaagt cggtgtcgga tcttgcaaaa
     1621 tataagaaac tcttcatcga tccg
//
LOCUS       DAOSWW010000688         1643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_64895_length_1643_cov_3.516373, whole genome shotgun sequence.
ACCESSION   DAOSWW010000688 DAOSWW010000000
VERSION     DAOSWW010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64895_length_1643_cov_3.516373"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PK442_11990"
     CDS             complement(<1..978)
                     /locus_tag="PK442_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO88415.1"
                     /translation="MKFNLKLNRLGARILAVVIVLLVVSVGGIGFYAMQVGGRGLQDA
                     ARQYETTLSENLAYKLNSTFNRFDGMLQALSALVTTRFTSMPNPEVADIVIPQFGAQN
                     GRFMNDLGVQSPEARSLFIIFNPDVFGTAKTHMVGFQRKDANTLFMYIDEKTIPAAEL
                     VDRKNPSTSWFWTPLDTKRPYWADVSRTEDGGEIVTYAMPVLIGEDIVAVAGMTFDFS
                     FVRETLKAVKIYDSGYPFLMNRNLKYLYHPTLSFDGPGFREVSNGYFAKYADDILNKG
                     KGRIDYPWESDQKTMSYYTMENGYILFVSASIAESLAAVGAMQRAVYIGI"
     regulatory      complement(1075..1159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1215..>1643)
                     /locus_tag="PK442_11995"
     CDS             complement(1215..>1643)
                     /locus_tag="PK442_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88416.1"
                     /translation="LDLSTILQPGLAGEVKKTVDLDDTTGTSSPYLGQYLSTSACAAL
                     AVHASMAATEGRLPEGYLSVGRRLDLEHDAPAMTGTTVIVKATLREIRGNRLIFDILG
                     SDALGVIFKGVNERVVVNRIGLEERGTERAQQLKALREKL"
BASE COUNT          311 a          456 c          503 g          373 t
ORIGIN      
        1 gatgcctatg tagacggcgc gctgcatcgc gccgaccgcg gcgagcgact cggcgatgct
       61 tgcggaaacg aagaggatgt agccgttctc catggtgtag tacgacatcg tcttctggtc
      121 gctctcccac ggatagtcga tccgcccttt gcccttattg aggatgtcgt cggcgtactt
      181 ggcgaagtaa ccgttcgata cttcccggaa gccggggccg tcgaacgaga gcgtcgggtg
      241 gtagaggtac ttgaggttgc ggttcatcag gaaggggtag ccgctgtcgt agatctttac
      301 ggctttgagc gtctcgcgta caaaggagaa atcgaaggtc atccccgcga cggcgacgat
      361 atcttccccg attaagaccg gcattgcgta cgtgacgatc tcccctccgt cttcagtacg
      421 gctgacgtcc gcccagtagg ggcgttttgt gtccaagggt gtccagaacc atgaagtcga
      481 gggatttttc cggtcgacaa gctccgcggc ggggatagtc ttttcgtcga tgtacatgaa
      541 gagggtattc gcgtccttgc gctggaaacc gaccatgtgc gtcttcgccg tgccgaagac
      601 gtccgggttg aagatgatga agaggctgcg cgcttcgggg gactggacgc cgaggtcgtt
      661 catgaacctg ccgttctgcg ccccgaactg cgggatcacg atgtcggcga cctcggggtt
      721 cggcatactg gtgaatcggg tcgttacaag ggcggagaga gcctgaagca tgccgtcgaa
      781 gcggttgaac gtggagttga gcttgtaggc gaggttctcc gagagcgtcg tttcgtactg
      841 ccgcgccgca tcctggagtc cgcggcctcc gacctgcatc gcataaaagc cgatgcctcc
      901 tactgaaaca accagcagga caatgactac tgcaagtatt cgtgcgccga gcctgttcag
      961 tttcagattg aatttcaacg caattcctcc ttttaaaaaa gtagactagg tcttacttgc
     1021 tctaaaaaca gcagcctccg gttttcggtt cggaggctgc gttgaaatac ttcctccggc
     1081 ggctcggcgg tcgttgcccg aaggcggtag acccgtagct ttgcgtcccc gtctttcgaa
     1141 gggtttgccc ttgttcggtt cagtgagaca gcgtatccgg cgttccttgg cggaaggcgc
     1201 cgggcgcctc ttgcttacag cttttcgcgc agcgccttca gctgttgcgc acgttccgtt
     1261 cctcgctcct caaggccgat acggttgaca acgactcttt cgttgacgcc cttgaaaata
     1321 acgccgagag cgtcggagcc aaggatgtcg aagataaggc ggttcccgcg gatctcccgg
     1381 agcgttgcct ttacaatgac ggtcgttccg gtcatggcgg gcgcatcgtg ctcaagatcg
     1441 agcctgcgtc ctacggagag gtacccttcc ggcagacgtc cttcggtcgc cgccatggat
     1501 gcatgcaccg cgagcgccgc gcatgcggaa gttgaaagat actgtccgag atacggagag
     1561 gacgtccctg tcgtatcgtc gaggtccacc gtctttttca cttcgccggc caaaccgggc
     1621 tgcagaatcg tggaaagatc gag
//
LOCUS       DAOSWW010000689         1643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_64949_length_1643_cov_1.964106, whole genome shotgun sequence.
ACCESSION   DAOSWW010000689 DAOSWW010000000
VERSION     DAOSWW010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64949_length_1643_cov_1.964106"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK442_12000"
     CDS             <1..189
                     /locus_tag="PK442_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88417.1"
                     /translation="LVKLVIVGWNLFECLERNFPDDLEERLHFFRGESDFYPDFDEAL
                     REKVVKHYRAHNKEPLWE"
     gene            286..993
                     /locus_tag="PK442_12005"
     CDS             286..993
                     /locus_tag="PK442_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88418.1"
                     /translation="MKDAIRKLVEDAGLRFGEFDGGETPFYGERRGEGGIVVRGSTVR
                     EIRRSIADLRRKEERNFAKHAVEGAGLRFSTLERSVWENGEPVVNILYRGWNDEGEEV
                     VSGSCLGDVLEAVEKFQEKETLKIEEVEERDRQWMLRLKILRKTPLELAEGHIDAGHF
                     FRWLKEYVRHYEDCAVDRTDWLRMFESEVVPCGSKEFTLPATAAKGGKAISYKDFQYR
                     RITHKDGEEEYAFFHGG"
BASE COUNT          382 a          399 c          535 g          327 t
ORIGIN      
        1 ctggtgaagc tcgtgatcgt cggatggaac cttttcgaat gcctcgaaag aaacttcccg
       61 gatgacctgg aagagcggct ccattttttc cggggagagt cggattttta ccccgacttc
      121 gacgaggccc tgcgggaaaa agttgtaaag cactaccgcg cacacaacaa agagccgtta
      181 tgggaatgag gcacaacctc tgatggcacg ctgtttccgc ctgcgaggtt ccaatgatca
      241 aaggctggga acgcaactat tgacaacgag acgggagggt acgggatgaa ggatgcgatc
      301 cgcaagctgg tggaggatgc ggggctccgg ttcggggagt tcgacggcgg ggaaacaccg
      361 ttctacggag agcggcgggg cgaaggcggc attgtggtga ggggaagcac cgtccgcgag
      421 atcagaagga gcatcgcgga tctgaggcgg aaggaagaaa ggaacttcgc caaacacgca
      481 gtggaaggcg cggggctgcg cttctccact ctcgaaagga gcgtttggga gaacggggag
      541 ccggtcgtga acatcctcta cagagggtgg aacgacgagg gagaggaggt cgtttccgga
      601 tcgtgcctcg gcgacgttct ggaagccgtc gaaaagtttc aggagaagga gacactgaag
      661 atcgaagagg tggaggaacg ggaccggcag tggatgctgc gcctgaagat tctccggaag
      721 acgcccctcg aactggctga gggtcacatc gacgccgggc acttcttccg gtggttgaag
      781 gagtatgtcc ggcactacga ggactgcgcc gtcgaccgga ccgactggct ccgcatgttc
      841 gagagcgagg tagtcccctg cggatcgaaa gaattcacac ttccggcgac cgcggcaaaa
      901 ggcggaaagg ctatctccta caaggatttc caatacaggc gcatcacaca caaggacgga
      961 gaagaggagt acgccttctt tcacggcgga tgaaacggaa ccggacatct ggctcgatca
     1021 ctgagtcggc atcatatttt tgccgatgga gatgagcata tgacaacgga ttgcgttcaa
     1081 ctgatcaagt gcccctgttg cggagcaggg atattgtcgc cattgcactc cgatacgaaa
     1141 agaaatcctt caagcgcttt tttcaagggg gcggatcgat aggtgtgaaa attttgagca
     1201 ggttgcgact tttccgcctg ctttgatgta caatgatacg tattctatga gcctgttgaa
     1261 ctatctgttg tagttttttt cacgccgcgg gcggcatgat tggaacaact gtgcgctgcc
     1321 cgcacgcttc gcgcccatga acgacactcg gttgcggctg gagtcgccgg gaggtgagaa
     1381 tgcgccgtag agggctttcg caggatcatg cgcttcaaga tcccccgaat cgcctcggga
     1441 gggccggttg ccgcgcatgc gcggctcgtt gagagtgttt gatcagcaac cgaagggagg
     1501 aaggcctgat gaaaaaaagc tttggctcga aattggcgac ggtggcgctc gcagcagttg
     1561 ttgtattttc cgtacaggcg ggattcggaa gcgctgcatt tgcggcgccg gacaccgagc
     1621 gctacacgta cttcgacaat ctg
//
LOCUS       DAOSWW010000690         1642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_64971_length_1642_cov_4.456207, whole genome shotgun sequence.
ACCESSION   DAOSWW010000690 DAOSWW010000000
VERSION     DAOSWW010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64971_length_1642_cov_4.456207"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PK442_12010"
     CDS             <1..381
                     /locus_tag="PK442_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOO88419.1"
                     /translation="DPAAIEKTWRVLPIGFWKGSGLSIALDLIGALLSKGNTVRQIGE
                     LGDEIAVTQIFLAFNAEGMYGKEYVESVVEGVLSDLKQAAPSEPGGEILYPGEKEGRT
                     RRENLELGIPVNKEIWEGILRELA"
     gene            443..1441
                     /locus_tag="PK442_12015"
     CDS             443..1441
                     /locus_tag="PK442_12015"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO88420.1"
                     /translation="MKKCTLLFLVAVLAALFVLPAQADPLKIVAAHNQTSLENPYQHG
                     MMKFKEVLEKISGGEISVDVHAGTIGTNEDELVEKLQLGAADVVVASPGFMTKIGIPE
                     VDLFSLLYLFNSFGHWEKAVDGEAGQTLAKIINEKSHNTFRIAAYWSAGVRNYYGKKP
                     INTMDDLKGMKIRTQMSGVVADFWKQTGAIPTQVAWGELYQALQQGIVDCAENDYTNF
                     SLLDHHKTVNGKFITETEHDYTTRFVLMNGKKFDSLTDQQKAWIVEAMKEATAKEREI
                     TYNMLSKSKERVIADGGTVNTIDKAPFIAIAVPIQDKLAEKLGIQNLVEMVRAAGK"
BASE COUNT          362 a          513 c          477 g          290 t
ORIGIN      
        1 gaccccgcgg cgatcgagaa aacgtggcgc gtgcttccga tcggcttctg gaagggttcc
       61 ggcctttcca tcgcgctcga cctgatcggc gcgctcctct cgaaggggaa caccgtccgt
      121 caaatcggcg aactcggcga cgagatcgcg gtcacccaga tcttcctcgc cttcaacgcg
      181 gaggggatgt acgggaagga gtacgtggaa tccgtcgtgg agggcgtcct ctccgacctg
      241 aaacaagcgg ctccgtcgga accggggggc gaaatcctct accccggcga gaaggagggg
      301 cgcacccgca gggagaacct cgaactgggc atccccgtca acaaagagat ctgggagggt
      361 atcctccggg agctcgctta aagttcgtgc tttcgcggaa gttttttccg catatcttaa
      421 ccaagagaaa ggggtaccaa aaatgaagaa gtgcacactg ttgttccttg tagctgttct
      481 tgcggcgctc tttgtccttc ccgcgcaggc cgatccgctg aagatcgtgg cggcgcacaa
      541 ccagacgtcc cttgagaatc cgtaccagca cggcatgatg aagttcaagg aagtgcttga
      601 gaagatctcc ggcggcgaaa tctccgtcga cgtccacgcc ggaaccatcg gcaccaacga
      661 ggacgagctc gtcgagaagc tccagctcgg cgcggccgac gtcgtcgtgg cctcccccgg
      721 cttcatgacg aagatcggca tccccgaggt cgacctcttc tcgcttctct acctcttcaa
      781 cagcttcggg cactgggaga aggccgtcga cggcgaagcc ggacagacgc tggcgaagat
      841 catcaacgag aagtcgcaca acacgttccg tatcgccgcc tactggtccg ccggagtccg
      901 caactactac ggcaagaaac ccatcaacac gatggacgac ctcaagggca tgaagatccg
      961 cacccagatg tccggcgtcg tggccgactt ctggaagcag accggcgcca ttccgacgca
     1021 ggtcgcctgg ggcgagctct accaggcgct gcagcagggc atcgtcgact gcgccgagaa
     1081 cgactacacc aacttcagcc ttcttgacca ccacaagacg gtcaacggca agttcatcac
     1141 cgagaccgag cacgactaca ccacccgctt cgtgctgatg aacggcaaga agttcgactc
     1201 ccttaccgac cagcagaagg cgtggatcgt cgaggccatg aaggaagcca cggcgaagga
     1261 gcgcgagatc acctacaaca tgctctccaa gtcgaaggag cgcgtcatcg ccgacggcgg
     1321 cacggtgaac acgatcgaca aggcgccctt catcgccatc gccgtgccca ttcaggacaa
     1381 gctcgccgag aagctcggca tccagaatct cgtcgagatg gtccgcgctg cgggtaaata
     1441 agcctttttc tcctcctgcg ggaggtcagc ggacattccc cgtgaaaccc gggcggggaa
     1501 tgtccctttt tcttcgcccc gaaaggcgac ggacgaggtt ttcaaagagg aggagaaccg
     1561 tgaaaagact gatttcgact cttgtgcgca ttcaggaggc cctgggaacg ctgctgctcg
     1621 ccatcttctt cctggccatc ct
//
LOCUS       DAOSWW010000691         1641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_65034_length_1641_cov_3.815889, whole genome shotgun sequence.
ACCESSION   DAOSWW010000691 DAOSWW010000000
VERSION     DAOSWW010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65034_length_1641_cov_3.815889"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PK442_12020"
     CDS             <1..665
                     /locus_tag="PK442_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO88421.1"
                     /translation="VREGRRPVLATFLSGTITDFTGVGAYMEIEGLRRLLGEGTTASG
                     AYLQVDSLYWDDFWRRVKETPRIGSVTTTAGARESFSKTTEEMMGVSQMLYLVFSLIV
                     SFGVIYNGARIALSERSRSLATLRVLGFTRREVTTILLSELGLLTLLSIGPGLLLGTW
                     MAKSILESVDTETMRFPLILGARTYVVAVLTVFVSTVFSFVVVGRRIAGLDLLAVLKS
                     AE"
     gene            686..>1641
                     /locus_tag="PK442_12025"
     CDS             686..>1641
                     /locus_tag="PK442_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOO88422.1"
                     /translation="MKSKLNRNGVNGKRIWKKLATTLAVCAVIGAAVFMLWPKPLEVE
                     TGLAERRPFTASVVEEGKTRIRHRYTVSSPVNGFLNRTALRPGDVVRAGETVLAVMEA
                     ESSGLLTPRLRAEAEARLKTAEAARALRKSELERAKVSLDLAKKQFERLDRLLPSGGI
                     SRQEWDIAESKMQVAIREVKTAEFALRMAEFEVQQVRSSLIQGEGGGASESVRILSPV
                     DGYVLSVFEESARTVPAGTPLMEVGDPDDIEGEVDLFSVDAAVVAQEAEALIEGWGGD
                     APLRGRVALIERSGFTKVSALGVEEQRVKVRIDFLDPVPPGYR"
BASE COUNT          295 a          457 c          570 g          319 t
ORIGIN      
        1 aagtgcgaga agggcgccgt cccgtgctcg caaccttcct ctccggaact atcaccgact
       61 ttaccggggt gggcgcctat atggagatcg aaggactgcg caggcttctt ggagagggta
      121 cgacagcaag cggcgcttac ctgcaggtgg actcgctgta ctgggatgat ttttggcgga
      181 gggtgaaaga aactccccgc atcggttcgg tgaccacgac cgcaggcgcc agggagagtt
      241 tcagcaagac caccgaggag atgatgggcg tgtcgcagat gctctatctt gtcttttccc
      301 tcatcgtctc cttcggagtg atctacaacg gggcgcgcat cgctctttcg gagcgctccc
      361 gctcgctggc gacgctgcgc gtgctcgggt tcacccgccg cgaagtgacg accattctcc
      421 tttcggaact ggggcttctc acgcttcttt cgattggtcc cgggctcctg ctggggacgt
      481 ggatggcgaa atccattcta gagtcggtgg acaccgagac catgcgcttc cctctgatac
      541 tcggagcgag gacgtacgtg gtcgcagtgt tgaccgtgtt tgtgtccacg gttttttctt
      601 tcgtcgtggt gggacgacgg atcgccgggc tcgatctgct cgcggtgctg aaatccgctg
      661 aatagcgcga aacggggtgg agttggtgaa gtcgaaactg aacagaaacg gcgtcaacgg
      721 aaaaagaatc tggaaaaaac tcgcgacaac tcttgcagtg tgcgccgtca tcggcgcggc
      781 cgtcttcatg ctgtggccga aacctctgga agtggagacg gggcttgcgg agcgcaggcc
      841 cttcaccgcg agcgtcgtgg aggaaggaaa gacgcggata cgacaccggt acacggtctc
      901 ctcccctgtg aacggctttc tgaaccgtac ggcgctgcgc cccggagacg tcgttcgtgc
      961 gggcgagacc gttcttgcgg ttatggaggc ggaatcctcg ggcctgctga cgcctcggct
     1021 tcgtgcggaa gccgaagcac ggctgaagac ggccgaagcg gcgcgcgcgt tgcgaaagtc
     1081 ggagcttgaa cgggcgaagg tgagcctcga tctcgcgaag aagcagttcg agcgcctgga
     1141 cagactgctt ccttcgggcg gaatttcgag gcaggagtgg gatatcgccg agagcaaaat
     1201 gcaggtcgcc atccgagagg tgaagacggc ggaatttgcg ctccgtatgg cggagttcga
     1261 ggtgcagcaa gtcagatcct cgctcatcca gggggagggg ggcggggcgt ccgagtccgt
     1321 tcgcatcctc tcccctgtgg acggctacgt gctgagcgtc ttcgaggaga gcgcccggac
     1381 cgttcccgcc gggacgccgc tcatggaggt gggggatccc gacgatatcg agggcgaggt
     1441 cgacctcttc tccgtggacg ccgccgtcgt ggcgcaagag gctgaggcgc ttatcgaagg
     1501 atggggcgga gacgcgccac tccgcgggcg cgtcgcgctc atcgagcgga gcggtttcac
     1561 caaggtatcg gcgctggggg ttgaggagca gcgggtgaag gtccggatcg acttcctcga
     1621 tcccgtgccg ccggggtatc g
//
LOCUS       DAOSWW010000692         1638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_65235_length_1638_cov_3.670246, whole genome shotgun sequence.
ACCESSION   DAOSWW010000692 DAOSWW010000000
VERSION     DAOSWW010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65235_length_1638_cov_3.670246"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..844
                     /locus_tag="PK442_12030"
     CDS             131..844
                     /locus_tag="PK442_12030"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOO88423.1"
                     /translation="MKEIYYSITNDVFIPFPDYVRGVVVARGVKNGPSPAELTALLRE
                     AEEQLRGKLGSGNIAEHPRIASWREAFRKTGVKPAEFRSSIEAMARRVAKGQELPSIN
                     ALVDIGNVLSLRHLVPTGGHALDDVRGDIVLRAATGEETFVPFGETETEHPLPGEIIF
                     AEGDVVMTRRWSWRQGVHTLTLPESTSIEFNVDGLPPVTSGEVESVCGEVVELVSRFC
                     GGTFETGLLSAASPRMRLL"
     gene            complement(1048..>1638)
                     /locus_tag="PK442_12035"
     CDS             complement(1048..>1638)
                     /locus_tag="PK442_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88424.1"
                     /translation="FWLAGWWGLSSATFFLSSLYLHPDLWIRISHIYFQPARIVSYAL
                     AFYLGARAWRDGWFDGPRPGFGAIALSGLATVAGSWSVIFLAMNFPVKETLALKALHG
                     MSHSFASLSIAVFFLFLCRALFDRPSPVWRTLSEASYGIYWLHQMILMPAVYLLIPWN
                     LPIGIKFILALGGTYILCAFLTIHGLKKLPVLRRLF"
BASE COUNT          312 a          497 c          573 g          256 t
ORIGIN      
        1 cccagcgctg ctcgacgagg cgaaggagga gctccgcgag cgactggacg gcgcttcgta
       61 ccgctgcgcc atccccgacg aggttgttcc gaaggcgatc cagccttcgg gcagatagga
      121 ggaacaacgc atgaaagaaa tttactacag cattacgaac gacgtcttca tcccgttccc
      181 cgattacgtc cggggggtcg tggtcgcgcg cggcgtgaag aacggtccct ctccggcgga
      241 gcttacagcg ctgctccgcg aggccgaaga gcagctgcgc ggaaagctcg gctccggtaa
      301 catcgcggag cacccgcgca tcgcgtcgtg gcgggaggcc ttccggaaga cgggcgtgaa
      361 gcccgccgag ttccgttcct ccatcgaggc catggcgcgc cgcgtcgcga aggggcagga
      421 gcttccgtcg atcaacgccc tcgtggatat cggcaacgtc ctctcgctcc gccatctcgt
      481 cccgacgggc gggcacgcgc tggacgacgt gcggggcgac atcgtcctcc gcgccgccac
      541 tggagaggag acatttgttc ccttcggcga gacggagacc gaacacccgc ttcccggcga
      601 gattatcttc gcggagggag atgtggtcat gacccggcgg tggagctggc ggcagggggt
      661 gcacaccctc acgctgcccg agtcgacctc catcgagttc aacgtggacg gactgccgcc
      721 ggtgacgtcc ggcgaggtgg agtccgtctg cggcgaggtc gtcgagctgg tgtcgcgctt
      781 ctgcggcggc acgttcgaga cggggctgct ttcggcggcc tctccccgga tgcgcctgct
      841 gtaagcaggc gagtaaacgg gagttttcct cgtttgtccg gcggaagcgt gccggaggtt
      901 ttgcgctgtc gatcgctcgc gtcctcgcag cgggataatg cggtatgatt ctggccgcta
      961 taacagttac ggattctccg aaaacacaaa cgcgcggccc ccatgcaaac tcgggagccg
     1021 cgcgtccttt gtccctgcac gggagcgtca gaagagccgc cgtagcaccg ggagcttctt
     1081 cagtccgtgg atcgtgagga acgcgcacag aatgtacgtg cccccgaggg cgaggatgaa
     1141 cttgatgccg atcgggaggt tccacggaat cagcaggtag acggcgggca tcaggatcat
     1201 ctggtggagc cagtagatgc cgtacgacgc ctcgctcagc gtcctccata ccggagacgg
     1261 acggtcgaag agcgcccggc agaggaagag gaagaagacc gcgatggaaa gggaggcgaa
     1321 ggagtggctc atgccgtgca gcgccttgag ggcaagcgtc tctttcacgg ggaagttcat
     1381 ggcgaggaag atcaccgacc acgaccccgc gaccgtggcc agccccgaga gcgcgatcgc
     1441 gccgaagccg ggccgggggc cgtcgaacca gccgtcgcgc cacgcccggg ctcccaggta
     1501 gaaggcgagg gcgtaggaga cgatccgggc gggctggaag tagatgtggc tgattcgtat
     1561 ccagagatcc gggtgcaagt agagggacga aaggaagaac gtggcggagg agaggcccca
     1621 ccaccccgca agccagaa
//
LOCUS       DAOSWW010000693         1637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_65314_length_1637_cov_2.762958, whole genome shotgun sequence.
ACCESSION   DAOSWW010000693 DAOSWW010000000
VERSION     DAOSWW010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65314_length_1637_cov_2.762958"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..120
                     /locus_tag="PK442_12040"
     CDS             7..120
                     /locus_tag="PK442_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOO88425.1"
                     /translation="MPIFLHSKSEEELEIMRGIKRSFDPQGILNPGKLLGN"
     gene            200..1321
                     /locus_tag="PK442_12045"
     CDS             200..1321
                     /locus_tag="PK442_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88426.1"
                     /translation="MIDKNRFGILAVAFVWFTTHFGGGFASGVQVVQYFVSYGWYALF
                     LPVLSIAIVTAVLYYAWTFAAIYCTFDYREWGNKYFKPFEALFSNAYEVMYILILLIA
                     SAVAFATGGTVMQQILGTPYVMNTVIVAVCIFFLTIFGADLVRKAASTMAVIIIAGML
                     LIYVGNLIVNFPKLLAVLKEAPAPASFLDALWASLKYAGFQACAMGAYIAVADALKSR
                     EDAKKASIWGFIVNAGVLWLATAGILLHYPAILTEKVPVLFVAAHGGGGAIGTAIVSL
                     LIFLAVVSTGVSLIFGGARRIVGLWGKMNGSTVAGDRKVNIAASLIYVLITWGIALFG
                     LIPLIAKGYGYTGVLSFPLLIVPVLLIGVMRSGKWGKEL"
     gene            1438..>1637
                     /locus_tag="PK442_12050"
     CDS             1438..>1637
                     /locus_tag="PK442_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOO88427.1"
                     /translation="MERRGITTMKGNPMTLVGPGLQPGDKAPDFLVLDQTMAPKTLKD
                     YAGKFKVISVTPSLDTPVCDLQ"
BASE COUNT          322 a          512 c          467 g          336 t
ORIGIN      
        1 gtgaagatgc ccatcttcct tcactccaag tccgaagaag agctggagat tatgcgcggg
       61 atcaagcgat ccttcgaccc gcagggcata ttgaaccccg gcaagctgct gggaaactga
      121 cggcaacagc gggggcggcc tgtacaggac acccccggcg cactcatacc ttactgtaag
      181 cacgagagga gaagagatta tgatcgacaa gaacaggttc ggcattctcg cagtggcgtt
      241 cgtctggttc accacccact tcggcggcgg gttcgccagc ggcgtgcagg tagtccagta
      301 ttttgtgtcc tacgggtggt acgcgctctt cctgcccgtc ctctccatcg ccatcgtcac
      361 tgcggtgctc tactacgcat ggaccttcgc cgctatttac tgcaccttcg actaccgcga
      421 gtggggaaac aaatacttca aacccttcga ggcgctcttc tccaacgcct atgaagtcat
      481 gtacatcctc atcctgctca tcgcctcggc ggtggccttc gccaccggcg gaacggtgat
      541 gcagcagatc ctggggactc cctatgtcat gaacaccgtg atcgtcgccg tctgcatctt
      601 cttcctcacc atcttcggcg cggaccttgt caggaaggcc gcctccacaa tggcggtgat
      661 catcatcgcc ggaatgctgc tcatctacgt gggaaacctg atcgtcaact tcccgaagct
      721 gctcgcggtc ctcaaggagg ctcccgcgcc cgcgagcttc ctcgacgcgc tctgggcgtc
      781 gctcaaatac gcgggcttcc aggcgtgcgc gatgggcgcc tacatcgcgg tggccgacgc
      841 gctgaaaagc cgagaggacg cgaagaaggc ctcgatctgg ggcttcatcg tcaacgcggg
      901 agtgctctgg cttgcgacgg cgggcatact cctccactac cccgccatcc ttaccgagaa
      961 agttcccgtg ctcttcgtgg ccgcgcacgg cggcggcggc gcgataggca ccgccatcgt
     1021 ctcgctgctc atcttcctcg ccgtggtctc caccggcgtc agcctcattt tcggcggcgc
     1081 gcggcgcatc gtgggtctgt gggggaaaat gaacggatcg acagtggccg gagacagaaa
     1141 agtgaacatc gcagcatccc tgatctacgt cctcatcacg tggggcatcg cgctcttcgg
     1201 tcttattccg ctgatcgcca aagggtacgg gtacaccgga gtcctctcct ttccgctgct
     1261 catcgtcccg gtgctgctca tcggcgtcat gcgctccggg aagtggggca aagagctgta
     1321 gagagtcgaa aagtcgcctc atttattcgc gccatacagg gcgggggggt atattcccct
     1381 ccgccctctt tttgtgatac aataagccac agaaattatt tcggaggtga atacgctatg
     1441 gaaagaagag gaataaccac aatgaagggg aatccgatga cgctcgtcgg ccccggcctg
     1501 caaccgggag acaaagcgcc ggatttcctc gtgctcgacc agacaatggc cccgaagacg
     1561 ctgaaggact acgcggggaa gttcaaggtc atatcggtga ccccctcgct ggataccccc
     1621 gtgtgcgacc tccagat
//
LOCUS       DAOSWW010000694         1637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_65342_length_1637_cov_1.749684, whole genome shotgun sequence.
ACCESSION   DAOSWW010000694 DAOSWW010000000
VERSION     DAOSWW010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65342_length_1637_cov_1.749684"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..305
                     /locus_tag="PK442_12055"
     CDS             21..305
                     /locus_tag="PK442_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88428.1"
                     /translation="MPICGKGLPGVGFPRSEFEAIKDMGQGTVAREIVDDTMPGGPRT
                     RTIEYRVPFSKCDREEDYRRAWDAFSRREDTQGTGLLDGIRGLFGRSRQS"
     gene            302..>1637
                     /locus_tag="PK442_12060"
     CDS             302..>1637
                     /locus_tag="PK442_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO88429.1"
                     /translation="MIPSILAREVRRGVEDYIRTTFPVTSPFFNGVLEEFLRRENGLF
                     RGPFIFLGLPFSPGKGEGEFFPEVPLGYPPYLHQEKAFARMAHPRGRSTVIATGTGSG
                     KTECFLWPVLDYCLKHRGRRGIKAIFIYPMNALASDQAQRTARAIWDNPELKERVTAG
                     LYVGHDPEDALDQPVKAMERDKVITCRYTLRENPPDILLTNYKMLDFLLLRPADRELW
                     RYNTPDTLKYLVVDELHTFDGAQGTDLSCLLRRLKSRLDIPSGHLCCVGTSATLGEKE
                     RASEVLGYAASVFGERFDADALVTEERLSAGEFLENDMAVRFDLPGPEASGGLDPEFF
                     DSPLDYLERQAFLWFGGEGRRKDGESGDEWRVRLGTLLRGHVFFQNFLKVLGGRPMEY
                     SDLLGRLVKVAPRLGEGEDASYAPLVLDSLLSLVSHARKQEEGRLRPLVTVQV"
BASE COUNT          285 a          510 c          557 g          285 t
ORIGIN      
        1 agtccctgct ctgacgacgc gtgccgatct gcggcaaggg gctgcccggc gtcggcttcc
       61 cacgaagcga gttcgaggcg atcaaggaca tgggccaagg gacggtcgcc agggagattg
      121 tggacgacac gatgcccggc ggtccccgga cgcggacgat cgaataccgt gtgccttttt
      181 caaagtgcga ccgtgaggag gactaccgcc gcgcctggga cgccttttcc cggagggagg
      241 acacacaagg gaccggcctt cttgacggca tcaggggcct gttcgggagg agccgacagt
      301 catgattccc tcaatactcg cccgggaagt gcgccggggc gtcgaagact atatcaggac
      361 cacctttccc gtaacgtccc cgttcttcaa tggggtgctc gaggagttcc tccggcggga
      421 aaacggcctt ttccgcggcc cattcatctt tctgggactg cccttcagcc ccggaaaggg
      481 ggagggcgag ttcttcccgg aagtcccgct cgggtaccct ccgtatctgc atcaggagaa
      541 ggccttcgcc cgcatggcgc accctcgggg aaggtccacg gtgatcgcca cgggaacggg
      601 ctcgggaaag acggagtgct tcctctggcc cgtgctcgac tactgcctga agcaccgggg
      661 gcgccggggg atcaaggcga tcttcatcta cccgatgaac gccctggcgt cggaccaggc
      721 gcagcgcacc gcgcgggcga tatgggacaa tccggagctg aaggagaggg tgaccgccgg
      781 gctgtacgtc gggcacgacc ctgaggacgc gctcgaccag ccggtcaagg cgatggagcg
      841 ggacaaggtg atcacatgcc gctacaccct gcgggagaat cctccggata ttctgctgac
      901 caactacaag atgctggact ttctgctgct tcgccccgcc gacagggagc tgtggcgtta
      961 caacacgccc gacactttga agtacctggt ggtggacgag ctgcacacgt tcgacggcgc
     1021 gcaggggacc gacctctcgt gcctgctccg gaggctgaaa tcgcggctcg atattccgtc
     1081 cggacacctg tgctgcgtcg gcacatcggc caccctcggg gagaaggagc gcgcctccga
     1141 agtcctcggg tacgccgcgt ccgttttcgg cgagcggttc gacgccgacg ccctcgttac
     1201 ggaggagcgg ctctccgcgg gggagtttct ggagaacgac atggccgtcc gtttcgatct
     1261 gcccggcccc gaagcgagcg gcgggctgga ccccgagttc ttcgattcgc ccctcgacta
     1321 cctcgaacgg caggctttcc tgtggttcgg cggcgaaggg cgcaggaagg acggcgagag
     1381 cggggacgag tggagagtcc ggctgggaac gctgctccgg gggcacgtct tttttcagaa
     1441 cttcctcaag gtcctcggcg gaaggcccat ggaatactcc gaccttctgg gaaggctggt
     1501 caaagtcgct ccccgcttgg gagaggggga ggatgcttcc tacgctcccc tcgttctcga
     1561 cagcctgctc tcccttgtga gccacgcgag gaagcaggag gaggggcggc tccggccgct
     1621 ggtcacggtg caggtgc
//
LOCUS       DAOSWW010000695         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_65371_length_1636_cov_3.346616, whole genome shotgun sequence.
ACCESSION   DAOSWW010000695 DAOSWW010000000
VERSION     DAOSWW010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65371_length_1636_cov_3.346616"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1636
                     /locus_tag="PK442_12065"
     CDS             128..>1636
                     /locus_tag="PK442_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88430.1"
                     /translation="MPRKSVHAEDIVYFPERILSKVSRVKDYPLTLIEAPSGFGKTTS
                     FREYLRAHIPSSSRAFWYTSLGEPSSKAWGTICDLFDDVDPRVSRNLRSLDTPNRETM
                     ADVAAIMRECSCDRDSFVIIDNYQLVKSEVQSDIINAFSLHRNDALHIVFITQKLSAN
                     TTWRAPDIHNIGPVDFSFDKRSVQRYFALAGLTLSSQELETVCAATGGWIAALKLQML
                     HYKATGALAPANTMNDLIETSIWNRLSEEEKRFLLSVSLFECFSPRQASIMLEGVSND
                     VNIDEILRRNAFVPFVPDEGVYYMHSLLQDYLRDRFEQTGETFRRETYRRAGRACETV
                     GEYFHATQFHIETGNYEAILSMPFTTQYFYNFRERSVIDLFERLIDECPEETLLKYPL
                     ALVTFGQQFFRDRRMLPFSKTTALVQKLLDAPSGLSEKELLRIRGEFSILMSFPQFND
                     IAKMSEYHRRAYDSLRQLSDPPRSVIFGGAMPWTMSGASVLFLYWRESGALKK"
BASE COUNT          370 a          486 c          436 g          344 t
ORIGIN      
        1 agctgtattc actcctggag acagaaaata caaaatcatt atcaaaactg gataaaatgc
       61 agtgtttagg gtaccatagc aaccggaacc tcaggccacc ccattacgat gcacaggaga
      121 gagtacaatg ccccgaaaaa gcgtccacgc tgaagatatc gtctactttc cggaacggat
      181 tttgtcgaaa gtctcccgcg tcaaggacta tccgctgacg ctcatcgaag cgccttccgg
      241 attcggcaag acgacctcgt ttcgcgaata cctgcgcgcg cacatcccat cctcttcgcg
      301 cgcgttctgg tacacgagtc tcggggaacc atcctcgaaa gcctggggca ctatctgcga
      361 tctcttcgac gacgtcgacc cgagagtatc ccggaacctt cgaagcctcg acacgccgaa
      421 cagggaaaca atggcggatg tggccgcgat catgcgcgag tgttcctgcg accgcgattc
      481 ttttgtaatc atcgacaatt atcagctcgt aaaaagcgaa gtgcagtccg acatcatcaa
      541 cgctttttcc ctgcaccgca acgacgcgct ccacatcgtc ttcatcacgc agaagctctc
      601 cgcgaacacg acctggcgcg caccggacat tcacaacatc ggcccggtcg atttttcttt
      661 cgacaaacgg agcgtgcagc gttacttcgc gctggcgggc cttacgctct cgtctcaaga
      721 gctggagacg gtgtgcgccg ccactggcgg atggatcgca gcgctcaaac ttcagatgct
      781 ccactacaag gcgacgggag ctcttgctcc ggcaaacacc atgaacgacc tcatcgagac
      841 ctcgatatgg aaccgcctct cggaagagga aaaacgtttt ttgctttccg tctcgctgtt
      901 cgaatgtttt tccccgcgcc aggcgtcgat catgctcgaa ggcgtttcca acgatgtcaa
      961 tatcgatgaa atcctgcgac ggaacgcctt cgttcccttc gttccggacg agggcgtgta
     1021 ctatatgcac agcctccttc aggactatct gagggacagg ttcgaacaaa cgggcgagac
     1081 gttccggcgg gagacgtacc gcagggcggg gcgcgcctgc gagacggtcg gcgagtactt
     1141 tcacgcgacg cagttccaca tcgagaccgg gaactacgag gcgatcctct cgatgccgtt
     1201 cacaacgcag tatttttaca atttcaggga gcgaagcgtc atcgacctct tcgagcgcct
     1261 tatcgacgag tgcccggagg aaacgctgct gaaatacccg ctggctcttg tgacgttcgg
     1321 ccagcagttt ttcagggaca gaaggatgct cccgttttca aaaacgacgg cgctggtgca
     1381 gaaactcctc gacgctcctt ccggcctctc cgagaaggag ctgctgcgga tcagggggga
     1441 gttttccatc ctgatgtcct ttccccagtt caacgatatc gccaagatga gcgaatacca
     1501 caggcgggca tacgactccc tgcgtcaact gtcggaccct ccgcggtcgg ttattttcgg
     1561 tggggccatg ccgtggacga tgagcggagc gtcggtgctc ttcctctact ggagagagag
     1621 cggcgccctg aaaaaa
//
LOCUS       DAOSWW010000696         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_65543_length_1634_cov_2.424953, whole genome shotgun sequence.
ACCESSION   DAOSWW010000696 DAOSWW010000000
VERSION     DAOSWW010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65543_length_1634_cov_2.424953"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..964
                     /locus_tag="PK442_12070"
     CDS             5..964
                     /locus_tag="PK442_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88431.1"
                     /translation="MVGLLASGAFASDLNAYTIMPEKYASQVFEAFTAETGIKVNFMR
                     FSSGEALARVVAEKNNPQVDMILGGPADTYEAGIKEGVFEQYKPSEADMIPERFRSPE
                     NYWTGVGIIPLVFLTNGKFLKEKGLEAPASWNALLDPAYKNGLQMADARTSGTATERI
                     YALVKIMGEDEAFAYQKKLHGNIQMYTKSGAGGAMPIATGQAASGIFYIVDALEIQQQ
                     GHDVVLSYPVEGVSFGIEATGILKGAKNLENAKKFMDWASSKTFAQLLVDRKINYIPT
                     RTDVETTNPALDVSKVKLVEVDVAWKGANRKAFVDRWINEVIK"
     gene            1059..>1634
                     /locus_tag="PK442_12075"
     CDS             1059..>1634
                     /locus_tag="PK442_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO88432.1"
                     /translation="MTQPTAPHATSKVSLLKRDPALLPALIAIWISLAVFVLYPLFRL
                     FLTTFVVEGQLSFTNLAVVLESWYDRQAFVNSIWLATAVSVAGTFLGYVYAFAVTRIP
                     MPLWLKWFLGAVTTLPLISPPFTSSIALTLSLGPNGILLKLLGIGNFNFYGFWGTWMS
                     ETLTYFPVSFLTLAAVLTAIDPNLEDAGLSLG"
BASE COUNT          312 a          527 c          472 g          323 t
ORIGIN      
        1 cgttctggtc ggccttctgg cgtccggggc ctttgcctcg gatttgaacg cgtacaccat
       61 catgcccgag aaatacgcgt cgcaggtctt cgaggccttc accgccgaaa ccggcattaa
      121 ggtgaacttc atgcgcttct cgtccgggga ggcccttgcc agggtggtgg cggagaagaa
      181 caatccgcag gtcgacatga tcctcggcgg tcccgccgac acctacgaag ccggtatcaa
      241 agagggcgtc ttcgagcagt acaaaccctc cgaggccgat atgatcccgg agcgcttccg
      301 ttcgcccgag aactactgga ccggcgtggg catcatccct ctggtcttcc tgaccaacgg
      361 aaagttcctc aaggagaagg ggcttgaagc acccgcctcc tggaacgctc ttcttgaccc
      421 ggcgtacaag aacggcctcc agatggccga cgcccgcacc tccggcacgg cgaccgagcg
      481 catctacgcc ctcgtgaaga tcatgggcga ggacgaggcg ttcgcctatc agaagaagct
      541 ccacgggaac atccagatgt acaccaagag cggcgcgggc ggcgcgatgc ccatcgcgac
      601 cggacaggcc gcctccggaa tcttctacat cgtggacgcg ctcgagatcc agcagcaggg
      661 gcacgacgtg gtgctgagct accccgtcga gggagtctcc ttcggcatcg aggccacagg
      721 cattctgaag ggcgcgaaaa accttgaaaa cgcgaagaag ttcatggact gggcttcgtc
      781 gaagacgttc gcgcagctgc tcgtcgacag gaagatcaac tacatcccga cgcgcaccga
      841 cgtggagacc acgaaccccg cgctcgacgt ttctaaggtc aagctggtcg aggtcgacgt
      901 tgcgtggaag ggcgccaacc ggaaggcctt tgtcgaccgc tggatcaacg aggtcatcaa
      961 gtagcggact cattccggtt tttggaaaac ctgcccccgg cggacgctct ttccgccggg
     1021 ttttttacct ttgatgttca ggcaaggaga ttgaacttat gacacaacca acggcgccgc
     1081 acgcaacgag taaagtatct ctcctgaagc gggatcccgc gctgctcccc gcgctcatcg
     1141 ccatctggat atcgctcgcg gtcttcgttc tctacccgct cttccggctt tttctgacca
     1201 cgttcgtcgt ggaagggcag ctctcgttca cgaacctcgc agttgtgctc gaaagctggt
     1261 acgacagaca ggccttcgtc aacagcatct ggctggccac ggccgtctcc gtcgcgggga
     1321 ccttcctcgg atacgtctac gccttcgcgg tgacgcggat tccgatgcct ctctggctga
     1381 agtggttcct gggggccgtc accacgctcc ccctgatctc gccccccttc acgagcagca
     1441 tcgcgcttac cctctcgctg gggcccaacg gcatactgct gaagctgctg ggcatcggga
     1501 acttcaactt ctacggcttc tgggggacgt ggatgtcgga gactttaacc tatttccccg
     1561 tttcttttct caccctggcg gcggtgctca ccgcaatcga cccgaacctc gaggacgccg
     1621 ggctctcgct gggc
//
LOCUS       DAOSWW010000697         1633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_65582_length_1633_cov_3.534221, whole genome shotgun sequence.
ACCESSION   DAOSWW010000697 DAOSWW010000000
VERSION     DAOSWW010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65582_length_1633_cov_3.534221"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PK442_12080"
     CDS             complement(<1..474)
                     /locus_tag="PK442_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOO88433.1"
                     /translation="MHEHPLVGGIEAGGTKIVCAAGTGPGDLSRAVFPSGSDPAAALF
                     AVTDWFREEETRRGPLHAIGIGSFGPVDLDPASPTYGFITSTPKTGWRNTDILGTVRR
                     AFPALPVAFDTDVNAAALGEQRWGAAAGLDDFVYITIGTGIGAGVMAGGRLVHGLV"
     gene            complement(467..1036)
                     /locus_tag="PK442_12085"
     CDS             complement(467..1036)
                     /locus_tag="PK442_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOO88434.1"
                     /translation="MAKSVLAVLAEGFEEIEAITPVDVLRRLDVDVTVASLDGLVVKG
                     ARGISVQADTTLAEVAGRTFDLIVLPGGMPGAANLAASSLLGDMLKKQRAEGRMIAAI
                     CAAPAVVLHPLGLLGDEKTSCYPAFQRRLDEANRTEERLRVGEKLITAAGPGVAMEFA
                     LALAEILKGKEKTTELAKAMLVEGSRCNA"
     gene            1180..>1633
                     /locus_tag="PK442_12090"
     CDS             1180..>1633
                     /locus_tag="PK442_12090"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOO88435.1"
                     /translation="MTKERIGFIGLGIMGKRMALNLLKAGYSMTIRSGSEETRSEFAA
                     KGVQVAQTPAEVAAESDVIITMLPGSPEVEEIVLGEHGIINTVRAGSACIDTSSIDPV
                     TSVRIASELRKKGVFMLDAPVSGGQEKAESGTLSIMAGGDEEVFERCRA"
BASE COUNT          310 a          561 c          499 g          263 t
ORIGIN      
        1 aacaagcccg tggacaagcc gtccgcccgc catcaccccc gcgccgatgc cggtgccgat
       61 ggtgatgtag acgaagtcgt cgagacctgc cgccgccccc caccgctgct cgcccagcgc
      121 cgcggcgttc acgtcggtgt cgaaggcgac gggaagagca gggaaagcgc ggcgcactgt
      181 tccgaggata tcggtattcc gccatcctgt cttcggcgtc gaggtgatga acccgtatgt
      241 gggcgacgcg ggatcaaggt ccacggggcc gaacgagccg atgccgatgg cgtgcaacgg
      301 tccccggcgc gtctcctcct cgcggaacca gtccgtcacg gcgaaaagtg cggcggcggg
      361 gtcgctcccg gaggggaaga cggcccggct cagatcgccc ggcccggtcc ccgccgcgca
      421 gacgatcttc gtccccccgg cctcgattcc cccgacaagc ggatgttcat gcattacagc
      481 ggcttccttc caccagcatt gccttcgcca gttcagtggt cttctccttg cccttcagaa
      541 tctccgcgag ggccagcgcg aactccatgg cgacacccgg ccctgccgca gtgatcagct
      601 tctcgcctac acgcagacgc tcctcggtgc ggttggcctc gtcgaggcgg cgctggaaag
      661 caggatagca cgatgtcttc tcgtcgccca gcagcccaag agggtgcagc actaccgcag
      721 gagccgcgca gattgccgcg atcatccgcc cctccgcacg ctgctttttc agcatatcgc
      781 cgagcagcga ggaagcagcc aggttcgccg ccccaggcat tccgcccggc agcacgatca
      841 ggtcgaaggt ccgtccggcg acctccgcaa gcgtcgtatc ggcctgaaca gaaattcccc
      901 gggccccctt caccacgagc ccgtcaagcg acgcgaccgt cacatcgaca tcaagccgcc
      961 gcagcacgtc gaccggcgta atcgcctcga tctcctcgaa cccctcggca agcacagcga
     1021 gcacactctt cgccatcttc actcctcctt ctttatgaaa ccggtcggcc gttcccggcc
     1081 tttctttcca ttatacccga gcggcggctc gtgccgtcca ttagcagatg atacaatgat
     1141 gaagcacagc gcgcacttca acagaaggga cggtacggca tgacgaaaga acgaatcgga
     1201 ttcatcggcc tggggatcat ggggaagcgc atggcgctga acctgctcaa ggcggggtac
     1261 tccatgacca tccgcagcgg cagcgaggag acccggagcg agtttgccgc gaagggcgtc
     1321 caggtggcgc agacgcccgc cgaggtggcc gccgaaagcg acgtgatcat caccatgctt
     1381 ccgggatctc ccgaggtgga ggagatcgtc ctgggcgaac acggcatcat caacaccgtc
     1441 cgagcgggga gcgcctgcat cgacacaagt tccatcgatc cggtgacgtc ggtacgcatc
     1501 gcctcggaac tgcggaagaa aggcgtcttt atgctcgacg ctccggtcag cgggggtcaa
     1561 gagaaggcag agtccggcac gctgtccatc atggcgggag gcgacgaaga ggtcttcgag
     1621 cgctgccgcg cca
//
LOCUS       DAOSWW010000698         1628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_65919_length_1628_cov_3.278449, whole genome shotgun sequence.
ACCESSION   DAOSWW010000698 DAOSWW010000000
VERSION     DAOSWW010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65919_length_1628_cov_3.278449"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /gene="trkA"
                     /locus_tag="PK442_12095"
     CDS             complement(<1..1288)
                     /gene="trkA"
                     /locus_tag="PK442_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOO88436.1"
                     /translation="MFSFMGRNALREGRKPIIRAPEGPPEGFLEMKIVVVGGGEVGYN
                     VANTLSEEEHDVTVVEEDEERAEKVDNELDVITVRGSGARPQVLEEAGIFPGCDVDIL
                     VACTDRDEVNILACWIAKRAGVKRVISRARALEFTDSPTWAKDLGIDVMNSPERSVAR
                     EILELLFVSSAVHTAELLDGRSGIYALRVSPNSALVGLALKDLRTLYPNFIAIIVYIE
                     RDGTGYVPYGEMVIEANDLCYIVTSREQAWKLEEIYQMKKSRPLKRVLIIGGGKLGFQ
                     VAWKLESQFRNLDIRLIDHNREKCERLASELKRTLVLCGDGADESLLRQEGIEGADGL
                     VCATESDEANLLFAVIGKALGARKTIAVVRRKIYMKLDNYMSVDAIVNPNSALASVIL
                     RHARYKSGAGVLSIIEKIDAEMLELTVPAASPAVGRT"
     gene            complement(1388..>1628)
                     /locus_tag="PK442_12100"
     CDS             complement(1388..>1628)
                     /locus_tag="PK442_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO88437.1"
                     /translation="EGRLPVFAVNFEGRDNGLVAGRLSEEFGIETRPGLHCAPLAHKT
                     LGTFPGGALRLSPGYFSTEEDVDATLSALRTILDS"
BASE COUNT          261 a          560 c          512 g          295 t
ORIGIN      
        1 tggtgcgccc cacggcgggg ctcgccgccg ggaccgtcag ttcgagcatc tcggcgtcga
       61 tcttctcgat gatggagagc acccccgctc ccgacttgta gcgcgcgtgg cgcagaatga
      121 ccgaggccag cgcgctgttc gggttgacga tggcgtcgac ggacatgtag ttgtccagct
      181 tcatgtagat cttgcgcctg accaccgcga tggtcttccg ggcgccgagc gccttgccga
      241 tgaccgcgaa gagcagattc gcctcgtcgc tctccgtggc gcagaccagc ccgtccgcgc
      301 cttcgatccc ctcctgtcgg agcaggctct cgtcggcgcc gtcgccgcag aggacgagcg
      361 tgcgtttgag ttcggaggcc agccgctcgc acttttcgcg gttgtggtcg atcaggcgga
      421 tgtccaggtt gcggaactgg ctctccagct tccacgcgac ctgaaacccc agcttgccgc
      481 cgccgatgat cagcacgcgc ttcagcgggc ggctcttctt catctggtag atctcctcca
      541 gcttccacgc ctgctcgcgg gaggtgacga tgtagcagag gtcgttcgcc tcgatgacca
      601 tctcgccgta cggaacgtac cccgtcccgt cccgctcgat gtacacgatg atggcgatga
      661 agttggggta gagcgtccgc aggtccttca gcgcgagccc gaccagcgcg gagttcggcg
      721 acacgcggag cgcgtagatc cccgacctgc cgtcgagcag ctccgcggtg tgcaccgccg
      781 agctgacgaa gagcagctcc aggatttcac gggccacgga gcgctccggc gagttcatca
      841 cgtcgatccc caggtccttg gcccaggtgg ggctgtcggt gaattcgagc gcccgcgcgc
      901 gggagatcac gcgctttacg ccggcgcgct tggcgatcca gcaggcgagg atgttcacct
      961 cgtcgcggtc ggtgcaggcg acgaggatgt ccacatcgca cccggggaag attcccgcct
     1021 cctccagcac ctgcggcctg gcgccgcttc cgcgcacagt gatgacgtcg agttcgttgt
     1081 cgaccttttc agcccgctcc tcgtcctctt cgacgaccgt gacgtcgtgc tcctcttcgg
     1141 aaagcgtgtt cgccacgttg tacccgactt ctcctccgcc caccacaact atcttcatct
     1201 ctaaaaatcc ctccggcggc ccctcggggg ccctgatgat cggctttctt ccctcccgga
     1261 gcgcgtttcg gcccatgaaa gagaacaaca atccgccgcc cgcccgttgg gggcgcgcag
     1321 ctcagggaca agatacccta attcgccgga aaagaccata ccccctccgt cgtgtccgcg
     1381 gaaaagatca cgaatcgagg atcgtacgaa gcgccgagag cgtcgcgtcc acatcctcct
     1441 ccgtcgagaa gtagcccgga ctgagccgga gggcgccgcc ggggaaggtc ccgagggtct
     1501 tatgggcgag cggggcgcag tggaggccgg ggcgcgtctc gatgccgaac tcctcggaga
     1561 gacgcccggc gaccagaccg ttgtcgcgcc cctcgaagtt caccgcgaag acggggaggc
     1621 gcccctcc
//
LOCUS       DAOSWW010000699         1617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_66632_length_1617_cov_3.503201, whole genome shotgun sequence.
ACCESSION   DAOSWW010000699 DAOSWW010000000
VERSION     DAOSWW010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66632_length_1617_cov_3.503201"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PK442_12105"
     CDS             <1..310
                     /locus_tag="PK442_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016113119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88438.1"
                     /translation="CGLLSALAGLMLVGRLDSAQPGAGVGYEFGAIAAVVLGGTSFSG
                     GKGTLAGTILGVLIMGVLENGMNLLNVNPFSEQVVKGVVIAAALVAYGSLGRKSRTAG
                     "
     gene            478..1356
                     /gene="rbsB"
                     /locus_tag="PK442_12110"
     CDS             478..1356
                     /gene="rbsB"
                     /locus_tag="PK442_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019259653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HOO88439.1"
                     /translation="MKKLFAVLLVVLLVAAPAAAAEKTLGLVISTLNNPFFVTLKQGA
                     EEAATKAGVKLVVLDAQDDSAKMTAAMEDLIQQKVDAILVNPTDSDAVTPSIMKANAA
                     GIPVLTIDRGASGGQVLCHVASDNVAGGVMAGEFLAKKLEGKGNVVELVGIPGTSAAR
                     DRGKGFNDAIATFKDIKVVASQAADFSRDKGLKVFENILQAQPEIAGVFAHNDEMILG
                     AVAAAEAAGRKGIIFVGFDAVDDAVAAVKAGKLAATVAQQPAVIGAMGVEKAVAHLGG
                     EKLPEFIPVPLQIVVE"
     gene            1428..>1617
                     /locus_tag="PK442_12115"
     CDS             1428..>1617
                     /locus_tag="PK442_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88440.1"
                     /translation="MTLLLEMKNVHKRYPGVHALKGISYSLRAGELHALVGENGAGKS
                     TLVKVLGGVVAPDEGEILI"
BASE COUNT          310 a          515 c          538 g          254 t
ORIGIN      
        1 ctgcggcctc ctctccgcgc tcgcgggact aatgctcgta ggaaggctcg actccgcgca
       61 gcccggcgcg ggcgtcggct acgaattcgg cgcgatcgcc gccgtcgtgc tcggcggaac
      121 cagcttctcg ggaggcaagg gaacgctcgc cggaaccatc ctcggcgtgc tgatcatggg
      181 cgtgctggag aacgggatga acctgctcaa cgtcaatccc ttctccgaac aggtggtgaa
      241 gggggtggtc atcgcggcgg ccctcgtggc ctacggaagc ctggggcgga agagccggac
      301 ggcggggtag cgcctgcttg ccgtattccg gacagccgca cggctcaagg aagtccgcga
      361 agagcgcatg cacatcttga acgattcata cgcgcttccc ggcgtaccaa acctgtttcc
      421 acaacgacgc ggaggcgtga aacgccccgc gcgaaaattc aaggaggtgt ccgtttcatg
      481 aaaaagttgt ttgccgtact gctggtggtt ctgctcgtcg ccgctcccgc ggcggcggcg
      541 gagaagacgc tcggcctggt gatctccacg ctgaacaacc ccttcttcgt cacgctgaag
      601 cagggggccg aagaggccgc aacaaaggcg ggcgtgaagc tcgtggtact ggacgctcag
      661 gacgactcgg cgaagatgac cgccgcgatg gaagacctga ttcagcagaa ggtcgacgcc
      721 attctggtga acccgaccga ctccgacgcc gtcacgccct ccatcatgaa ggcgaacgcc
      781 gcgggcatcc ccgtgctcac catcgacagg ggcgcgtccg gcggacaggt tctctgccac
      841 gtggccagcg acaacgtcgc gggcggcgtc atggccggag agttcctcgc caagaagctt
      901 gaaggcaagg ggaacgtcgt cgagctggtc ggcatccccg gaacctccgc cgccagagac
      961 cgcggcaagg gattcaacga cgctattgcc acattcaagg acatcaaggt cgtcgcctcg
     1021 caggccgccg acttcagccg cgacaagggg ctcaaggtct tcgagaacat cctccaggcg
     1081 cagccggaga tcgcgggcgt cttcgcccat aacgacgaga tgatcctcgg cgcggtcgcc
     1141 gcggcagagg ccgccggacg caagggcatc attttcgtcg gcttcgacgc ggtggacgac
     1201 gcggtcgccg ccgtcaaagc gggcaaactt gccgcgacgg tcgcccagca gcctgcggtc
     1261 atcggcgcga tgggcgtcga gaaggcggtc gcgcacctcg gcggagagaa gctgcccgag
     1321 ttcatccccg tcccgctgca gatcgtcgtc gaataggccg tacaccagat gaagtaccgg
     1381 gacgccgtcg ttcaagggcg gcgtccctcc caccggaggt tgatggcgtg acactcttgc
     1441 tggagatgaa gaacgtacac aagcgctacc cgggagtcca cgcgctgaag gggatctcat
     1501 actccctgcg cgcgggggaa ctccatgcgc tcgtcggcga aaacggcgcg ggaaagtcca
     1561 ctctcgtgaa ggtactcggc ggcgtggtcg ctcccgacga aggggagatc ctgatac
//
LOCUS       DAOSWW010000700         1616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_66697_length_1616_cov_3.191544, whole genome shotgun sequence.
ACCESSION   DAOSWW010000700 DAOSWW010000000
VERSION     DAOSWW010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66697_length_1616_cov_3.191544"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PK442_12120"
     CDS             complement(<1..813)
                     /locus_tag="PK442_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88441.1"
                     /translation="MLLLFLFAMFLPAEAESALQLFSGNQCLGNVNSLQQGAFSLVSA
                     TDMASLLELPAVAKDDTLVVTAGRNKIQLVSGASAAWLDVELVPLAASCVQRNGQWFL
                     ESGSALKLFHSLLSRSGKNVSLRWGTEVSDGAPAPLVTPPEPTKTPSSPVRDAPAVSA
                     PSSARPLLKGVRWGIDDDKIRVVLDLEGTGEPEVQRGAGSMKVFFSSGPWPVPGIPSP
                     YREVNAGAMNFGDRLMIDFASSLVVHEILSLEAPKRLVIDFRRSAASGAPVVP"
     gene            complement(821..>1616)
                     /locus_tag="PK442_12125"
     CDS             complement(821..>1616)
                     /locus_tag="PK442_12125"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOO88442.1"
                     /translation="SSGDEMEMGGYGDESRFLASLQPRATALEAVRRTAMVLGGEPLP
                     TGKYDLLLDPESSASFVEVLGELFLASNIHKNKSFLKGKLGERIASPALTLVDDGTLK
                     GGIGAAPFDGEGIPCSRTCLLSGGVVESWLYNLKYARIDGVASTGNAARSVSGTPDVD
                     CSNLFLLPGKWTPQDLLLQVGSGIMVTELLGLHTMNPVSGDFSLGIKGVRIKDGTLSE
                     PVAGMTVAGNLKDVLRSIDLVGNDFKFFGSIGSCSLVIRDVTAAGT"
BASE COUNT          369 a          489 c          475 g          283 t
ORIGIN      
        1 agggactacc ggcgctcccg atgcggcgct tcgtctgaag tcgatgacaa gtctcttcgg
       61 agcttccaga gagaggatct cgtggacgac aagcgaggaa gcgaagtcga tcatcagacg
      121 atcgccgaag ttcatcgcac ccgcgttcac ttcccggtaa ggggaaggaa ttccgggaac
      181 gggccagggc cctgaagaga agaagacctt catgctgccg gcgccgcgct gaacttcagg
      241 ttcgccggtc ccttcaaggt ccagtacaac cctgatcttg tcgtcgtcaa tcccccagcg
      301 gacccctttc agaagaggac gggctgaaga cggggcggag acggcaggcg cgtcgcgaac
      361 gggggaagag ggtgttttcg tcggctcggg gggcgttacg agaggagcgg gcgctccgtc
      421 gcttacttca gttccccagc gcagcgagac gtttttcccg gaccgggaga gaagagagtg
      481 aaaaagcttg agcgccgagc cgctttccaa aaaccactga ccgtttcgct gaacgcacga
      541 agcggccagc ggaaccagct ccacgtccag ccatgctgct gaggcgcccg atacgagctg
      601 tatcttgttt ctccccgccg tcacgacgag cgtatcatcc tttgcaacgg ccggcaactc
      661 cagaagcgat gccatgtccg ttgcggagac gagggaaaac gcgccctgct gcaaggagtt
      721 gacattcccc agacactgat tgcccgaaaa gagctgcaac gcgctctccg cctccgccgg
      781 aaggaacatg gcgaaaagga acaggagaag caacaggact ttacgttccg gcagccgtaa
      841 catcacgaat caccagagaa caggagccga tgctcccgaa aaacttaaaa tcgttgccga
      901 cgagatcgat tgagcgcagt acatccttga ggtttccggc aaccgtcatc cccgctacag
      961 gttccgaaag cgttccgtcc ttgatacgca cgcctttgat cccaagcgaa aaatcaccgc
     1021 tcacgggatt catcgtatgc agcccgagca gctcggtcac catgataccg ctccccacct
     1081 ggagcaaaag atcctgagga gtccactttc ccggaagaag gaagagattc gagcaatcca
     1141 catcgggcgt cccggagacg gagcgcgcgg cgtttcccgt agacgcaacc ccatcgattc
     1201 gcgcatactt caggttgtag agccacgatt cgacaactcc cccggagagg agacaggtcc
     1261 ttgagcacgg aatgccttct ccgtcgaacg gggcggcgcc gattcctcct ttgagcgtac
     1321 cgtcgtcgac gagggtaagc gccggggagg cgatcctttc gccgagctta cccttcagga
     1381 acgacttgtt cttatgaatg ttggaggcga gaaagagctc gccgagcact tcgacgaatg
     1441 acgcggaaga ctcgggatca aggagcagat cgtacttccc tgtgggcagc ggctcgccgc
     1501 cgagcaccat ggcggtacgc ctgacagcct ccagagctgt cgcacggggc tgcagagacg
     1561 caagaaaccg gctttcgtcc ccgtatccgc ccatctccat ctcgtcgccg gatgaa
//
LOCUS       DAOSWW010000701         1616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_66699_length_1616_cov_3.105061, whole genome shotgun sequence.
ACCESSION   DAOSWW010000701 DAOSWW010000000
VERSION     DAOSWW010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66699_length_1616_cov_3.105061"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1470)
                     /locus_tag="PK442_12130"
     CDS             complement(85..1470)
                     /locus_tag="PK442_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOO88443.1"
                     /translation="MKTSEAFLVCLKVGARIEREILEVFLKERIESLGSWGERRSECV
                     GVKTVFQDKPEEAELLAGIPAVLEKIASASEPGVSVKRTIILLPEGKNVWDRDLITVL
                     KSPVFDIQGIFRMERLLTGEKRSDPEDLFRLEDVLKEAIRSWLGRCGKGEREFSKPVD
                     WKASLDAHLSESGYVSLFADPSTRRMALEVKEAIHSLKSRAEAIQKSAEKLPLSETNF
                     KEMANKGWSVFAGRIPSVLLLGESGSGKTLLAKWIGESLLPKQFERVNISAIQSSLVD
                     GELFGAAKGAFTDHPFDTQGLFLSQAGGVVFLDEIGDMEPACQTRLLTFIDDGLVRPL
                     GWHGQPFAAPLLLVAATNRPLKEWVSSKSASFREDLFYRFDHVIELPPLRERRGDLRL
                     LISVTLQDEDVNAGYAQGKGVRRISLDAIEALERMEMPGNFRELRGVLKRACFAARRE
                     KAETLCLRHFA"
BASE COUNT          383 a          503 c          364 g          366 t
ORIGIN      
        1 agatcgaaaa aaacatataa cctgcggcta caaacgtagg cgcaagagtc cagaacacca
       61 cagaacgatt ttgataagcc gaaatcaagc aaagtgccgc agacagagtg tttcggcttt
      121 ctctctgcgt gcggcgaagc aggcgcgttt cagaacacct cgaagctccc tgaagttgcc
      181 cggcatctcc atacgttcca acgcttcaat tgcgtcgagc gagatgcgcc gaaccccttt
      241 cccctgcgca tatcccgcgt tcacatcttc gtcctgcaac gtgacgctga taagtagccg
      301 aagatctccc cgccgttcgc ggagcggagg aagttcgatc acgtgatcga accggtagaa
      361 caggtcttca cggaacgagg cgcttttcga cgaaacccac tcttttaacg gccggttcgt
      421 ggcggccacc agaagaagcg gcgccgcaaa cggctggccg tgccagccga gcggccgcac
      481 aagcccgtcg tcgatgaatg tgagcaggcg tgtttggcag gcaggttcca tatcgccgat
      541 ctcgtccaga aagacaactc cgcctgcttg cgacaagaaa aggccttgag tatcgaacgg
      601 atgatccgta aacgctccct tcgcggcgcc gaacagctcc ccatccacca gagaagactg
      661 tatagcggaa atattgaccc gttcaaactg cttcggcaga agcgactctc cgatccactt
      721 cgcaagcagc gtctttcccg aacccgattc tccaagcaga aggaccgaag gaatcctgcc
      781 cgcgaaaacg ctccatcctt tattcgccat ttccttgaag tttgtttcac tcaaagggag
      841 tttttcagcg ctcttttgga ttgcctccgc cctacttttc agggaatgaa tggcctcctt
      901 gacctccagc gccattctgc gtgtggaagg gtcggcgaaa agactcacat acccgctttc
      961 tgaaaggtgc gcatcaagag aagccttcca gtccaccggc ttcgaaaact ctctctcccc
     1021 tttcccgcac cttccgagcc aggatcttat ggcctccttc aatacatcct caagacgaaa
     1081 caagtcttcg gggtcgctcc gtttctcccc ggtcagcagt ctctccatcc tgaaaatacc
     1141 ctgaatatca aaaaccggac ttttcagcac ggtgatcaga tctctgtccc acacattctt
     1201 cccctcggga agcaggataa tcgtccgctt cactgaaacg cccggttccg aagcggaggc
     1261 gattttttcg agcactgcag gaatacctgc gagcagctcg gcttcctcgg gcttgtcctg
     1321 aaaaaccgtt ttcacaccga cgcactcgct cctccgctca ccccacgagc caaggctttc
     1381 tatcctttcc ttcaaaaaaa cctctagtat ttctcgttct atacgtgccc cgaccttcaa
     1441 gcacacgagg aaggcttcac tcgtcttcat ccgttcccat ctccttgagc aaatatcctc
     1501 gaaagacttt cagttttttc ttctgcaacg taccgatcga cacatttccc ggaagcttct
     1561 ctttttctat gccggggaaa gacccttgca aaagagatcg aaggctttcg acaaaa
//
LOCUS       DAOSWW010000702         1613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_66911_length_1613_cov_5.599487, whole genome shotgun sequence.
ACCESSION   DAOSWW010000702 DAOSWW010000000
VERSION     DAOSWW010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66911_length_1613_cov_5.599487"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PK442_12135"
     CDS             complement(<1..385)
                     /locus_tag="PK442_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88444.1"
                     /translation="MSMLRALRRLLPAVLLPVFVLLCVCPARSQETAPAASGMTKATL
                     TLQWYPQTQFAGYYMAREKGFYAARGIDLEIRRGGSDVDTIGELREGKTDFATLFLTA
                     GIRYRSQSLPLVNVAQIVNRGNLLVI"
     gene            complement(397..825)
                     /locus_tag="PK442_12140"
     CDS             complement(397..825)
                     /locus_tag="PK442_12140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO88445.1"
                     /translation="MENTVRERVFPASLDLLGDIRAFILEEDGAEERLPPKRVAHLDV
                     AIEEIVVNICSYAYETPPGELTIRVEEKPEFFAVDFLDNGVAFDPLAMDEPDVSLPLE
                     KRDAGGLGILLVRRMMDEVHYSRKGTLNSLRIVVDTPRMP"
     gene            complement(840..1190)
                     /locus_tag="PK442_12145"
     CDS             complement(840..1190)
                     /locus_tag="PK442_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOO88446.1"
                     /translation="MFEMHFTVETWGRSVLLSGRLDARSAPDFEKAALAAADEQPEKR
                     LHMLLDFSGLEYISSAGLRSILVLAKRLSPSGGKVVLCGMSGVVEEVFGVSGFDSFLP
                     VTKSREEALRLLGE"
     gene            1422..>1613
                     /locus_tag="PK442_12150"
     CDS             1422..>1613
                     /locus_tag="PK442_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88447.1"
                     /translation="MDETTRGQKTASEIGRTAADSFLSGLYCAESALLAVAEARGTRS
                     ELIPNIATGFCSGMARTCGT"
BASE COUNT          361 a          501 c          456 g          295 t
ORIGIN      
        1 cgatcacgag caggttgccc cggttgacga tctgggcgac gttcaccagc ggcaggctct
       61 gggaacggta gcggatcccg gccgtcagga agagcgtcgc gaagtccgtc tttccttcgc
      121 gaagctctcc gatggtgtcg acgtccgagc cgccgcgccg gatttcgagg tcgataccgc
      181 gcgctgcgta gaagcccttc tcacgcgcca tgtagtaccc ggcgaactgc gtctgcgggt
      241 accactgaag cgtgagcgtc gccttcgtca tgccggaagc ggccggggcc gtctcctgac
      301 ttcgggcggg gcaaacgcag agaagaacaa acaccgggag aagaacggcc ggaagcagcc
      361 gccgaagggc gcgcaacatc gacatgtcca atccccctac ggcatccggg gcgtgtccac
      421 cacgatacgg aggctgttca acgtcccttt gcgggagtag tgcacttcgt ccatcattct
      481 ccgtaccagg aggatgccca gcccccccgc gtcgcgcttt tcaaggggaa gagaaacgtc
      541 aggctcgtcc atggcgagag ggtcgaacgc gaccccgtta tccagaaaat ccacggcgaa
      601 aaactcgggt ttctcctcga cccgtatcgt cagctcgccc ggaggggttt cgtacgcgta
      661 ggaacagata ttcacgacga tctcctcgat cgcaacatcc aggtgcgcca cccgcttcgg
      721 cggaagacgc tcctccgccc cgtcctcctc cagaatgaag gcacggatat cgcccaacaa
      781 gtcgagagag gctggaaaaa cccgctctct gacggtgttt tccatcgtct tttcctcctc
      841 tactcgccga gaaggcgaag agcctcctcg cggctcttcg tcaccggaag aaagctgtca
      901 aagccggaaa cgccgaagac ctcctcgacg accccggaca ttccgcaaag caccaccttc
      961 cctcccgaag gagagagccg cttggcaagc acgagaatcg aacggagtcc cgcgctgctg
     1021 atgtactcga gtcctgaaaa atcgaggagc atgtgaagac gcttttcggg ctgttcatcg
     1081 gctgcggcga gcgcggcctt ctcaaaatcc ggagccgacc tggcatccag acgtcccgag
     1141 agcagaacac tccgccccca ggtttcgacc gtaaagtgca tttcgaacat agttcattcc
     1201 ccctgaaagc agcatacaga accaccacac tactgccgaa acgaatacag gcgttcttga
     1261 agttcattgt gcgcgttttt accggacgtg tcaagaaaag cggagaaacg ggatgcagtc
     1321 gaatattctc ttgccgattc cgcttcttgc gagacgacgg aatttatgtt attgtcgacc
     1381 aatatcgacc cgtcatcatg tcgtttttca aggaggaaaa gatggacgaa acaacacgag
     1441 gacagaaaac agcttccgaa attggaagga cagccgcgga ctcatttctc tcgggcctct
     1501 actgcgccga gagcgccctg cttgcagtgg cggaagcccg cgggacacgc tccgagctga
     1561 tcccgaacat cgcgacaggc ttttgcagcg ggatggcgcg tacctgcggg acc
//
LOCUS       DAOSWW010000703         1610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_67140_length_1610_cov_4.093891, whole genome shotgun sequence.
ACCESSION   DAOSWW010000703 DAOSWW010000000
VERSION     DAOSWW010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67140_length_1610_cov_4.093891"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK442_12155"
     CDS             complement(<1..442)
                     /locus_tag="PK442_12155"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOO88448.1"
                     /translation="MHVPSDIEIAQGSELRPIVEIAEKLGIGESELEQYGKYKAKISP
                     EVWDRVKGNADGKLILVTAITPTPAGEGKTTTTVGLAQALAKRGKKTSLAIREPSLGP
                     SFGVKGGAAGGGYSQVIPMEDINLHFTGDLHAITTAHNLISAMVD"
     gene            complement(497..>1610)
                     /locus_tag="PK442_12160"
     CDS             complement(497..>1610)
                     /locus_tag="PK442_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO88449.1"
                     /translation="AACGGVTSVIDFTVGASDSSIPDDIRNRLEDAKNAVVDYSFHGE
                     VVGWRPGRVDEMNEAIGLGVKSFKFFTAYGASGRRTDTGPLLQAFQALADMDGVAVVH
                     AEDDSMIETFSGLLSNEEWGRMESVAKVRPDVCEASAVNTVGWLAARTGVQCHIVHLS
                     SALGLEEAFYARERGADLTVETCPQYLLLTFDVYKGPEGHLYSAAPALRSSDDNAVLW
                     NALASGEIDFAATDHCPFTRTQKEWKGAFDRLPGGLPGVELLLPLLYSEGVLRGRLPL
                     CELARITSERAAKRYGLYPRKGSLLPGSDADIVLFDPEEVWHVQAGALRMNVDFSPYE
                     NLELIGKTWQTISRGEIIYSEGRFLGKKGRGVFLHR"
BASE COUNT          312 a          529 c          425 g          344 t
ORIGIN      
        1 tgtccaccat cgccgagatc aggttgtgcg ccgtggtgat cgcgtgaagg tcgccggtga
       61 agtggaggtt gatgtcctcc atggggatga cctggctgta accgcctccg gcggcccctc
      121 ccttgacgcc gaagctgggg cccagggacg gctcccggat ggcgaggctc gtcttcttcc
      181 cgcgcttcgc cagcgcctgc gcaagtccga ccgtcgtggt ggtcttccct tcgcccgcgg
      241 gcgtcggcgt gatcgccgtc accaatatca gcttcccgtc cgcgttcccc ttcacccgat
      301 cccatacttc cggcgatatt ttcgccttgt acttcccgta ctgctcaagc tccgactctc
      361 cgattcccag cttctccgct atctccacta tcggccgcag ttccgatccc tgcgctatct
      421 ctatgtcgct cggcacatgc attacttctc agtcctcctt gataaaatac cccttcatgt
      481 tggcgcgggg taacgattaa cggtgcaaaa acactcctcg tccttttttt cccaggaaac
      541 gcccttccga gtagataatt tccccccgtg aaatagtttg ccacgtcttt ccgatcagtt
      601 caaggttttc atacggagag aaatcaacat tcattcgaag cgccccggcc tgtacgtgcc
      661 agacctcttc cgggtcaaag agcacgatgt cggcgtccga accgggcagc aggctcccct
      721 tccgcgggta cagaccgtaa cgcttcgctg cgcgctccga ggtaatcctc gcaagttcgc
      781 acaacggaag cctgcctcga agcacacctt cggagtacaa cagcggaaga agcagttcca
      841 cgccgggcag tcctcccgga agccggtcga acgccccctt ccactctttc tgcgtccgtg
      901 tgaagggaca gtggtccgtg gcggcaaaat caatctcgcc cgaggcaaga gcgttccaaa
      961 gaacagcatt gtcgtctgaa ctcctcagtg cgggagccgc cgagtacaga tggccctcgg
     1021 gacctttgta gacgtcgaaa gtaagcaaca ggtattgagg acaggtctcg acagtcagat
     1081 cggcgcctct ttctctggca tagaacgcct cctcaagccc aagtgcagag ctgaggtgga
     1141 ctatatggca ctgcaccccg gttctggccg caagccagcc gacagtattc acggcggacg
     1201 cctcgcagac gtcggggcgt actttggcca cgctctccat acgcccccac tcctcgttcg
     1261 agagaaggcc ggagaacgtc tcaatcatgc tgtcgtcctc cgcatggacg acggcgacgc
     1321 catccatgtc ggccaaagcc tgaaaagcct ggagcagcgg ccccgtatcc gtacgtcgtc
     1381 cggaagcgcc gtaggccgta aaaaatttaa agctctttac gccgagaccg atcgcttcgt
     1441 tcatctcatc gactcttccg ggacgccatc ccacgacctc tccgtggaac gagtagtcta
     1501 caacggcgtt ttttgcgtcc tcaagacggt tccggatgtc gtcggggata ctgctgtcgg
     1561 aggcgccgac ggtaaagtcg ataaccgatg ttacccctcc gcacgcagca
//
LOCUS       DAOSWW010000704         1610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_67156_length_1610_cov_3.347910, whole genome shotgun sequence.
ACCESSION   DAOSWW010000704 DAOSWW010000000
VERSION     DAOSWW010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67156_length_1610_cov_3.347910"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..682)
                     /locus_tag="PK442_12165"
     CDS             complement(209..682)
                     /locus_tag="PK442_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOO88450.1"
                     /translation="MNTPDRYRYPAVLGYDASTQQYYVLWPDLPGCTTTGATEDEALE
                     HAREAMSLHLWGMEVDGDEIPAPTPLHRLDLGEYVEEGEKIVAVLVEVWMPSFRERME
                     TKAVNRTVTLPGWLDSQAKRAALNYSQILQDGIMERLKISRVVVKHKKSRVKTLV"
     gene            complement(679..927)
                     /locus_tag="PK442_12170"
     CDS             complement(679..927)
                     /locus_tag="PK442_12170"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOO88451.1"
                     /translation="MKQPEIDRNKIYSSSEIIKILEADGWQLHGAEGSHYQFKHPAKK
                     GKVTVKHPDKSVPGPTAKSIWKQAGLLQPPSSRKETES"
     gene            complement(1020..1475)
                     /locus_tag="PK442_12175"
     CDS             complement(1020..1475)
                     /locus_tag="PK442_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOO88452.1"
                     /translation="MIIGTLAACERYYVLGERFRKALEFLKEHDVAAMEPGRYDIEGD
                     DVFALVQEYVTKTIDDCTLEAHRVYADVNYVAEGFEYLGYAPLERAGVPAVEYDPKTE
                     AAFFEKECDFILLRKGDIAIVFPEDAHMPQKRALVPATVRKVCIKVRVG"
BASE COUNT          287 a          510 c          426 g          387 t
ORIGIN      
        1 ccagcagcac cttggtttcg ctctccgtga gggtattccc ctcgatggcg ttgctcgacc
       61 aggtcaggcc gatgcggtag tacctccgta tctcgtcgag catcgctcct tcgaacggac
      121 gcaccccgtc gatcgccgct ttcagccggt ccactctttg gaagatcgtc attgcgcatg
      181 cctccgttct tttctccttc tccgcttctc acaccaacgt cttcactctg ctctttttgt
      241 gttttacgac caccctgctg atcttcagac gctccatgat accgtcctga aggatctgcg
      301 aatagttcaa ggcggcgcgc ttcgcctgac tgtccagcca cccgggcaga gtgaccgttc
      361 tgttcacggc cttcgtctcc atcctctcac ggaaggacgg catccagact tcgaccagca
      421 cggcgacgat cttttcgccc tcctccacgt attcgcccag gtcgagccga tgcaaggggg
      481 tgggagccgg gatttcatct ccgtcgacct ccatgcccca aaggtgcagc gacatggctt
      541 ctctcgcgtg ctccagcgct tcgtcttcgg ttgcaccggt cgtggtgcag ccgggaaggt
      601 cgggccagag gacgtaatac tgttgcgttg aagcatcata ccccagaacg gcggggtagc
      661 ggtatctgtc gggcgtgttc atgactccgt ctcctttcgg gaagaaggcg gctgaaggag
      721 ccccgcttgc ttccaaatcg atttcgcagt aggaccgggg acacttttat cggggtgctt
      781 tacggttacc ttgccctttt ttgcgggatg tttgaactga taatgactcc cctccgctcc
      841 gtgcagctgc catccgtcag cttcgaggat cttgatgatt tccgacgagg agtagatttt
      901 attgcgatcg atctccggct gtttcatccc cggccaccct ttcagagcag agtataacac
      961 gtgtaaaaaa catttgtcag cactgctcgt gatactgttc ttttcgctct ccgttccttt
     1021 cagccgaccc gtaccttgat gcagaccttg cggaccgtcg ccgggacgag agcgcgcttc
     1081 tgcggcatgt gggcgtcctc ggggaagacg atggcgatgt cgcccttccg cagaaggatg
     1141 aagtcgcact ccttctcgaa gaatgccgcc tccgtcttgg gatcgtactc gacggcaggg
     1201 actccggcgc gttcaagcgg cgcgtacccc agatactcga acccctcggc gacgtagttc
     1261 acgtcggcgt acacccgatg cgcctccagc gtgcagtcgt cgatggtctt cgtgacgtac
     1321 tcctgcacga gggcgaagac gtcgtctccc tcgatgtcgt agcgccccgg ctccatagcc
     1381 gccacgtcat gctccttcag aaattcgagc gccttccgga agcgctcccc caacacataa
     1441 taccgttcgc aagcagccag cgtgccgatg atcatgctct tccccgcctt tcggttgtat
     1501 cgaggcgctt tcgcgcccgg ctagtacgat agcatgaaga ggaagggcgc tacaagagcc
     1561 ctatttctct ttctccaatg atcgaaatcc tcagagagaa cgcaagtaac
//
LOCUS       DAOSWW010000705         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_67367_length_1607_cov_3.172680, whole genome shotgun sequence.
ACCESSION   DAOSWW010000705 DAOSWW010000000
VERSION     DAOSWW010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67367_length_1607_cov_3.172680"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PK442_12180"
     CDS             complement(<1..479)
                     /locus_tag="PK442_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOO88453.1"
                     /translation="MDSFLHKTDIAVQRILRIVVLFFFLALAFLLAYNVLLRLTNDLS
                     TFLTERGFSEAAGAVRGWLPITSMHWFDEIVELCFAALIFYGAASLWASGGHFSVGDW
                     ISPRLPYERLRNLYRVFVDLLGLAFLWVFFFYSLRLTFRATELTTVFQIHKSALYCC"
     gene            complement(482..>1607)
                     /locus_tag="PK442_12185"
     CDS             complement(482..>1607)
                     /locus_tag="PK442_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO88454.1"
                     /translation="TLLAIPFFILAGNLMNTGGITKRIFRFASAMVGHIPGGLGQVNI
                     VASVIFSGMSGSAVADAAGLGQIEHKAMVDDGYDPAVSASLVAVSSVIGPVIPPSIPF
                     VLYGAITGVSVARLFMAGFIPGILMSVGMMIALAILAKRRNYPCSACRAPRSDLWASF
                     KGAFWALMAPVIIIGGIMTGFFTPTEASVVVCAYAMILGFAYRDLHWKDMPDILFKSA
                     GQAVSLLFIIAAANFFGWLIIHQKIPDAIISNLMSYGATQAHVLLLIMGVILVLGCFL
                     EGNAIFLITLPIFIPVAKMFQIDLVNFGVVMTLLIMIGNLTPPVGMCLFAVDSFAKVG
                     ILPLSKEVVPYLVVILGVTFVIAFVPDIALFLPRLMMGGM"
BASE COUNT          426 a          446 c          468 g          267 t
ORIGIN      
        1 atacaacagt acagcgcaga cttatgaatc tggaagaccg tggtcaactc cgtggcgcga
       61 aacgtcagac gcagcgagta gaagaagaat acccacagaa acgcgagtcc gagaaggtcg
      121 acaaacacac ggtagagatt tcggagtctc tcgtacggaa ggcgcggcga aatccagtcc
      181 cctacgctga aatggcctcc gctcgcccag agggatgccg ccccgtagaa gatgagcgct
      241 gcgaaacaga gctccactat ttcgtcgaac cagtgcatcg aagtaattgg aagccaccct
      301 ctgacggctc ccgccgcttc gctgaaaccg cgctcggtca gaaaagtcga cagatcgttc
      361 gtcaggcgca gcaaaacgtt gtatgccagc aagaaagcca gcgcaaggaa gaaaaaaaga
      421 acgacgattc ggagaatacg ctggacggca atgtcggttt tatggaggaa agagtccatt
      481 gttacattcc tcccatcatc agacgcggca ggaagagcgc gatgtcgggg acaaaagcga
      541 tgacgaaggt cacgccgagt atgacgacga gatacgggac aacctctttt gaaagaggaa
      601 gtatcccgac cttcgcgaaa ctgtccaccg cgaagaggca catgccaaca ggcggcgtca
      661 ggttgccgat catgatgagc agcgtcatca ccacgccgaa gttgacgagg tcgatctgga
      721 acattttcgc gacagggatg aagatcggca gcgtgatcag gaagattgcg tttccctcaa
      781 ggaagcagcc gagaacaaga atcacaccca taataagcag cagcacgtgc gcctgtgtgg
      841 cgccgtagct catcaggttc gagatgattg cgtcgggaat cttctgatga atgatcagcc
      901 acccgaaaaa attggcggct gcgatgataa agagcaggct cacggcctgc cccgcggatt
      961 taaaaaggat gtccggcata tccttccagt ggagatcacg gtaggcgaag ccaaggatca
     1021 tggcgtacgc gcagaccact acggacgcct ccgtaggcgt gaagaagccg gtcatgatgc
     1081 cgccgatgat gatgacaggc gccatgagcg cccagaaagc tcctttgaag ctcgcccaca
     1141 agtcgcttcg aggcgcccgg caggcggaac acggataatt tcgccgtttc gcaaggattg
     1201 cgagcgcaat catcattcca acactcatca ggataccggg gataaacccc gccatgaaga
     1261 ggcgggcgac cgagacgccc gtgatcgccc cgtagaggac gaaggggatg ctgggaggta
     1321 tgaccggtcc gatgaccgat gagaccgcca ccagagaggc cgacaccgca ggatcgtagc
     1381 cgtcgtccac catcgccttg tgctcgatct gccccagccc ggccgcgtcg gcgaccgccg
     1441 agccggacat cccggagaag atgacgcttg ctacgatatt cacctgcccc aggcctcccg
     1501 ggatatgccc gaccatggcg ctcgcaaagc ggaagattct cttggtaatt ccgccggtgt
     1561 tcatcaggtt tcccgcgagg atgaagaaag gaatcgccaa aagtgta
//
LOCUS       DAOSWW010000706         1604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_67609_length_1604_cov_3.094254, whole genome shotgun sequence.
ACCESSION   DAOSWW010000706 DAOSWW010000000
VERSION     DAOSWW010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67609_length_1604_cov_3.094254"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PK442_12190"
     CDS             complement(<1..644)
                     /locus_tag="PK442_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO88455.1"
                     /translation="MLVQNILNGISLGSVYALIAVGFALIFNVLKFSNFSQGGVMTVT
                     AYAGYVASRYLSDRLDSLALFAAVLAAAALSGAVIAAAVERLAFRRLRRTKSEPVYYF
                     ISSITMGILLENLVTIYASSNFYSYPRFFTTSAVPLFGVNVAVTDMMMFALSIASLLA
                     LLFILHRTKIGLGIRAACFDVNTVQLMGMNPDFLVTTALMISGALGGVSGVFLG"
     gene            complement(754..>1604)
                     /locus_tag="PK442_12195"
     CDS             complement(754..>1604)
                     /locus_tag="PK442_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88456.1"
                     /translation="VSNIGLALGPIANKAGVPVVGSFIDPRVTVGEDGKPHAAMFLMQ
                     PSSIQYSEIMASYTIDVLGLKKVGVLYDQSNAFAVSLMKPYVDYIKAAGGEIVSEQVY
                     TKGDKDFKTQLLKIKDAGADCLYFPNYIQDAVLAMNQRKQVGLDVPVIGGLDFAPPFA
                     SLVNDPEATNDIYLANNFSETEPQLKEVWEIYKTKYGEDPVNKVFLGYDKVLLIREAM
                     KLGGGTSAAQVMAGLSQVKELQGTTGVLTISPETHQPVGLSMVMYKIEKGKYVELGRY
                     VPEKHK"
BASE COUNT          306 a          482 c          527 g          289 t
ORIGIN      
        1 atccccagga agacgccgct gacgcccccc agcgcgcccg agatcatcag cgcggtggtc
       61 accaggaagt ccgggttcat ccccatgagc tgcacggtgt tcacgtcgaa gcaggcggcg
      121 cggatgccca gcccgatctt cgtccggtgg aggatgaaga gcagcgcgag cagcgaggcg
      181 atggagaggg cgaacatcat catgtccgtc acggcgacgt tcacgccgaa cagcggcacg
      241 gccgaggtgg tgaagaaacg ggggtaggag tagaagttgc tgctggcgta gatcgtgacc
      301 aggttctcca gcaggatgcc catcgtgatc gacgagatga agtagtacac cggctcgctc
      361 ttcgtccggc gaagccgccg gaacgccaga cgctcgacgg ccgcggcgat aaccgccccc
      421 gaaagcgcgg ccgccgcgag caccgccgcg aacagggcaa gcgaatccag ccggtccgag
      481 aggtagcggg aggcgacgta ccccgcgtac gcggtcacgg tcatcacgcc gccctgcgag
      541 aagttcgaga acttgaggac gttgaagatg agcgcgaacc ccaccgcgat gagcgcgtag
      601 acggatccga gggagatacc gttgaggatg ttctgcacca gcatgaaacc agctcctttg
      661 cccctcccgg cggcggaagg atgggaagaa atgagaggcc gccccgcttt tcgaaagaga
      721 aagggcggcc tctaaattat agtatcaggt cgcctactta tgcttctcgg ggacgtagcg
      781 gcccagttcg acgtacttgc ccttttcgat cttgtacatg accatggaga ggccgacggg
      841 ctggtgcgtc tccggagaga tggtgagaac gcctgtggtt ccctgaagct ccttcacctg
      901 cgacagcccc gccattacct gcgccgcgga ggtcccgccg ccgagcttca tggcctcccg
      961 gatgaggagg accttgtcgt agccaaggaa gaccttgttc acgggatcct cgccgtactt
     1021 cgtcttgtag atctcccaga cttccttgag ctggggctcc gtttcggaga agttgttggc
     1081 gaggtagatg tcgttggtcg cctcggggtc gttgacgagc gaggcgaacg gcggcgcgaa
     1141 gtccagtccg ccgatcacgg gaacgtcgag gccgacctgc ttgcgctggt tcatcgccag
     1201 cacggcgtcc tgaatgtagt tggggaagta caggcagtcc gcgcccgcgt ccttgatctt
     1261 cagcagctgc gtcttgaagt ccttgtcgcc cttcgtgtac acctgctccg agacgatctc
     1321 gccgcctgcg gccttgatgt agtcgacgta cggtttcatc agggagaccg cgaaggcgtt
     1381 cgactggtcg tagagcacgc cgaccttctt caggccgagc acgtcgatcg tatagctggc
     1441 catgatctcg gagtactgga tgctggaggg ctgcatcagg aacatcgccg catgcggctt
     1501 gccgtcctcg cccacggtga cgcgcgggtc gatgaagctg ccgaccacgg gaacgcccgc
     1561 cttgttggcg atggggccga gcgccagtcc gatattgctg acgg
//
LOCUS       DAOSWW010000707         1602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_67723_length_1602_cov_3.900452, whole genome shotgun sequence.
ACCESSION   DAOSWW010000707 DAOSWW010000000
VERSION     DAOSWW010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67723_length_1602_cov_3.900452"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PK442_12200"
     CDS             complement(<1..969)
                     /locus_tag="PK442_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88457.1"
                     /translation="MFSTTLRKLRAARLALLLTLFFACSGEAAPILYIVRIPLVLGEE
                     AQAVLPDGKTIPLGKVAALPTSSRWPGYTASKWAPPGSVAASAVNAVHLTLSVEKEKG
                     RTVSILPRHTVAPAAGEQSFIALDSPAGTGFFGGWAPPVATPVLVRRNDGALVPLEER
                     GLPREGDTLIFEVSESESPYLIDIENRPGGRVLGWYESGPRLLARVIRPLKGVGRFGG
                     TEFQNIGRIRANHSGVIDVSTTPRGVVGGFQILPFLHSKSQEMSSAWQLTQWLIIASP
                     TDRPLPGTAPLFSSNLVPGSQMTDVLWDMWSTYGRKPLVLRRRGGGA"
     gene            complement(999..1505)
                     /gene="mrtS"
                     /locus_tag="PK442_12205"
     CDS             complement(999..1505)
                     /gene="mrtS"
                     /locus_tag="PK442_12205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM system CAAX-type protease"
                     /protein_id="HOO88458.1"
                     /translation="MTAFIVFAFFLYFPYAWCALRGESPEKYALLWRLSPSSARELLL
                     VTGGALVPLTFISLFFFARYLPPWHVERILPGILSGLAAAVAEETFFRGWLQTLFAEK
                     YSEWKSILFASFFFGLAHIFQSPAAMLAFFPGIIMGLLRSRHGTVFSAILFHWFGNIW
                     SIWFYPHL"
BASE COUNT          386 a          491 c          466 g          259 t
ORIGIN      
        1 cgctcccccc ccccgacggc ggaggacgag cggcttgcgg ccgtacgtcg accacatgtc
       61 ccagaggacg tccgtcatct ggcttcccgg cacgaggttg gaggagaaaa gcggcgcagt
      121 cccgggaagc ggccggtcgg tcggcgacgc gataatcaac cactgcgtca gctgccaggc
      181 ggacgacatc tcctgggact tcgaatgcag aaacgggaga atctgaaaac ctccaacaac
      241 acctcgcggc gtcgtgctga cgtcgataac tccagaatga ttcgcccgta ttcttccgat
      301 attctggaac tcggtgcctc cgaaacgtcc cacgcccttc agagggcgga ttactcgggc
      361 gagcagcctc gggcctgatt cgtaccatcc gaggacgcgg ccgcccggac ggttctcgat
      421 gtcgatcaaa tacgggctct ccgactccga aacttcgaaa atgagcgtat ccccttcgcg
      481 gggaaggccg cgctcctcca ggggcacgag tgcgccgtca ttccgccgga cgagaaccgg
      541 cgttgccaca ggaggagccc agcccccgaa gaaacccgtt cccgccgggg agtcgagcgc
      601 gataaacgac tgctcgcccg ctgcgggagc cacggtatgc cgggggagaa tgctcacagt
      661 ccgccctttc tctttctcga cggagagcgt caggtgcact gcgttcacag ccgaagcggc
      721 gacgctcccg gggggcgccc atttcgaggc agtgtacccc ggccagcggc tcgacgtcgg
      781 cagcgcggca acctttccga gcggaatcgt ctttccgtcg ggcaaaaccg cctgcgcctc
      841 ctcgccaaga acaagaggga tgcgaacgat atagagaatc ggcgcggcct ctcccgaaca
      901 cgcgaagaag agggtgagaa gaagtgcgag acgcgccgca cgcagcttcc ggagtgtcgt
      961 actgaacatg gatcatacct ccctttttgt aggcgccgct aaagatgcgg ataaaaccag
     1021 atcgaccata tattgccgaa ccagtgaaac aaaattgccg aaaacaccgt tccgtgcctg
     1081 cttcgcaaaa gtcccatgat gatgcccgga aagaaggcga gcatcgccgc cggactctgg
     1141 aagatgtgcg caagcccgaa aaaaaaggag gcgaagagga tgctcttcca ctcggaatac
     1201 ttctcggcaa acagcgtctg aagccagccc cggaaaaatg tttcctcagc caccgccgcc
     1261 gccaggccgg agaggatgcc cggaaggatt cgttccacat gccagggagg gagatagcgg
     1321 gcaaaaaaga acagcgaaat gaaagtcaac ggaacaagag cgccccctgt aacaagaaga
     1381 agttcccggg ccgaggaagg cgaaagcctc cacaagagag cgtacttttc cggagattct
     1441 ccccgcagag cgcaccacgc atacggaaag taaagaaaaa aagcgaacac gatgaatgcc
     1501 gtcatttgaa ggctatcata gcacaccgct tctttctacg cacgctgccg ggcatcccgg
     1561 aaaaatgttt cttacgttgc attgtgcggt cctcaacgtc tt
//
LOCUS       DAOSWW010000708         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68014_length_1598_cov_3.005185, whole genome shotgun sequence.
ACCESSION   DAOSWW010000708 DAOSWW010000000
VERSION     DAOSWW010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68014_length_1598_cov_3.005185"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PK442_12210"
     CDS             <1..748
                     /locus_tag="PK442_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOO88459.1"
                     /translation="LGGDNVFVSSYAAFLIGKGYDQIRTAVAIPSLPVRIVGLGAGLT
                     AGEEGACHQMLEDIALMRVFPGMTVLVPADYTAAFALLKNVAGVQKPVYIRLGAAATP
                     DVYEEGDEAFFPGGGRVLREGTGVTLCCCGIMVHEALRAADVLARQNISAEVIDCYSV
                     SPLPVQAILDSVHRTGCCVTAEEHFASGGLGEAVASLTGANYPVPVKMVAVFDKFGQS
                     GSPPELKEYYGLTASQIVSAAMQAWIMRRR"
     gene            752..1447
                     /locus_tag="PK442_12215"
     CDS             752..1447
                     /locus_tag="PK442_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88460.1"
                     /translation="MDIRLSGITKVFEPDIVALEDIYLSVDSGEFLYLVGTTGSGKTT
                     LLRLISREYLPTRGQITVGDRNLRKLRSSELPYYRREVGVVFQDFKLLPNLTVFENVA
                     FVLEAMGIPPKLVESRSNEVIDQVGLWRRRFLKPPQLSGGEQQRVAIARAMANSPAVF
                     LADEPTGNLDLRTSEEIMRLLLSINASGTTVIVATHDQYLVDAYRQRLVELHNGRLKR
                     DERRGRYFVDGEL"
BASE COUNT          301 a          439 c          499 g          359 t
ORIGIN      
        1 tcttggaggg gataacgttt tcgtttcatc ctatgcggcc tttctcatcg gcaaggggta
       61 cgatcagatt cggacggcgg ttgccattcc ttcgctccct gtgcgcatcg tagggctcgg
      121 cgcagggctt acggcgggcg aagagggcgc ctgccaccag atgctcgaag atattgcgtt
      181 gatgcgggtt ttccccggaa tgaccgtcct cgttccagcg gattacaccg ccgcgttcgc
      241 gctcttaaaa aacgttgcag gcgtgcagaa gccggtgtat atccgccttg gcgccgcggc
      301 gacgcccgat gtctacgagg agggggacga agcgttcttc cccggtggcg gacgcgttct
      361 tcgggaggga accggcgtca cgctctgctg ttgtggtatt atggtccacg aagcgcttcg
      421 ggctgcggat gtgcttgccc ggcagaatat aagcgccgag gttattgact gttacagtgt
      481 ttcgcctctt cccgtccagg ctattctcga ttctgtccac aggacggggt gctgcgtgac
      541 tgcggaggag cacttcgcga gcggcggtct cggagaggct gtcgcatcgc tcacgggagc
      601 aaactacccc gtgccggtaa aaatggtcgc ggtgttcgac aagttcgggc agagcggttc
      661 tcctccggaa ctgaaggaat attacggcct gaccgcgtcg cagatagtga gcgccgcgat
      721 gcaggcatgg atcatgcgca ggaggtaatg gatggatatt cgcctgtctg gaatcacgaa
      781 ggtgttcgaa cccgatattg tcgcgctcga ggatatctat ctctcggtgg acagcggtga
      841 atttctctac cttgtgggga ccaccggatc gggaaagaca acgcttctca ggctgatctc
      901 cagggaatat ctccccacca ggggccagat caccgtcgga gacagaaatc tgaggaagct
      961 tcgctcttcc gagctcccgt actatcggag agaggtcggc gtagtctttc aggatttcaa
     1021 actcctcccg aacttgaccg tgttcgagaa tgtggcgttc gtactggaag cgatgggtat
     1081 tccgccgaag ctggtcgaga gcaggtccaa cgaagtgatc gatcaggtgg gactctggag
     1141 gaggcgtttt ttaaagccgc cccagctctc gggcggagaa cagcagcggg tggcgatcgc
     1201 gcgggcgatg gcgaactcgc cggctgtatt ccttgccgac gagcctaccg gcaaccttga
     1261 tctgcgaacc tcggaggaga tcatgcgtct cctgctctcc atcaacgcct cggggaccac
     1321 ggtgattgtc gcgacgcacg atcagtatct cgtggacgcc tataggcagc gcctcgtgga
     1381 gctgcacaac ggacggctga aacgggacga gcgcaggggg aggtatttcg tcgatggcga
     1441 gctttagata cgctttcaga gacacattcc gtcttctctt tcgccactgg ggtctgagcc
     1501 ttctcacgtt gctcacagcc gcttccgtcc tctatattct cggattcgcg tcgctcttcg
     1561 ctatgaacat gcggtacatg atctccagga tcgagggc
//
LOCUS       DAOSWW010000709         1597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68071_length_1597_cov_3.670558, whole genome shotgun sequence.
ACCESSION   DAOSWW010000709 DAOSWW010000000
VERSION     DAOSWW010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68071_length_1597_cov_3.670558"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK442_12220"
     CDS             complement(<1..231)
                     /locus_tag="PK442_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO88461.1"
                     /translation="MKRQNLRKALMVIFVLSLPVTMFYLSPLLTLQGAWKGIVTSSLV
                     FVGLSAVLAFFAGRVFCSWICPVGTLSEGIFRL"
     gene            453..728
                     /locus_tag="PK442_12225"
     CDS             453..728
                     /locus_tag="PK442_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88462.1"
                     /translation="MEHISSIITDFIVKNMNERGLSLYRTDEEKILALDDQYETCFKF
                     DLVLSDNDFSCAVLSQGEHGLVLRRRFNIPWTNAAEIREFMEFVRSL"
     gene            742..1209
                     /gene="msrB"
                     /locus_tag="PK442_12230"
     CDS             742..1209
                     /gene="msrB"
                     /locus_tag="PK442_12230"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOO88463.1"
                     /translation="MKLTLFVLCACTLLLFAVRAQAAAPERVEKSEDEWRAILTPEQF
                     EITRKKGTELAFSGAYWDHHEKGVYACVCCGAQLFSSEAKYDSGSGWPSYWMTIEGAP
                     VAEKRDVSFGMIRTEVLCARCDAHLGHLFTDGPPPTGLRYCINSASLTFIPAE"
BASE COUNT          337 a          438 c          459 g          363 t
ORIGIN      
        1 cagccggaaa attccttcgg acagcgtgcc taccgggcag atccagctgc agaacaccct
       61 tccggcgaag aaggcgagaa cagccgatag gcctacgaag acaaggctcg atgtcacaat
      121 gcccttccaa gctccctgca gcgtgaggag cggggagagg tagaacatcg tcacagggag
      181 cgagagcaca aaaatcacca tgagagcttt ccgcagattc tgccgtttca ttgcgatccc
      241 tccgtcattg aattgttcac tattatagca gatcatacct aatgggggta ttcttatttc
      301 ccccgtgaag acatcgttcc gtcgcacgcc ggcgcatact cttccgcatc gtgatcggat
      361 actgttcgca cctttcaccc atgacctttt tgcgctcaga gcgtacaata gtataaatca
      421 ccccggtgcg aggggaaggg aggatgtgtg cagtggagca tatttccagt atcataactg
      481 atttcatcgt gaaaaacatg aacgagagag gcttatcact ctacagaacc gacgaggaga
      541 aaatcttggc gctcgacgat cagtacgaga catgtttcaa gttcgacctt gtcctctcgg
      601 acaacgactt cagctgcgcg gtgctctcgc agggggagca cgggctcgtg ttgcgcagac
      661 gtttcaacat cccatggacg aacgccgcgg aaatcaggga gtttatggag ttcgtccgca
      721 gcctgtaaag gagggaaact catgaaactg acacttttcg ttctctgcgc ctgtacgctg
      781 cttcttttcg ccgtccgtgc gcaggcggcc gctccggaga gggtggagaa gtccgaggat
      841 gaatggcggg caatcctcac ccctgagcag ttcgagatca ccagaaaaaa ggggacggag
      901 ctcgcgtttt caggggcgta ctgggatcat catgagaaag gggtatatgc ctgcgtctgt
      961 tgcggagcgc agctcttttc ctcggaggcc aagtatgatt ccggctccgg atggccgagc
     1021 tactggatga cgatcgaggg agcgcccgtc gcggagaagc gcgatgtctc cttcgggatg
     1081 atccgcacgg aagttctttg cgcgcggtgc gacgcgcacc tggggcacct gttcacggat
     1141 ggtccgcccc ctacggggct tcgctactgt atcaactccg cttcgctgac ctttattcct
     1201 gcggagtgat ctgctccggt attgcttctg cagccgtccg cttcgtcagg atgcggacgg
     1261 ctgtccattt gcagggaacg cttcttgccc cggttggcgg agaacagcgc ttcttcatgc
     1321 acgcgcgggc tggacaaagg ggcttgaaat gtgttctttt cgggagcata atgctccatt
     1381 ggatgaacac gcccgcaacg cacgcctgtt cgcctcaaac gattctatgt gaaagggagt
     1441 ttgtatgcca tgcgcaggat ttttgatgga tatgcccgct ttttttcgtc tgtcgacctc
     1501 gacgagtacc gtgcgctcgc cggaggacag cgtcctcatt cgctcgtcat cacctgttcg
     1561 gacagccgga tcgtcccgga ggagctgttc gacgccc
//
LOCUS       DAOSWW010000710         1597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68107_length_1597_cov_2.367704, whole genome shotgun sequence.
ACCESSION   DAOSWW010000710 DAOSWW010000000
VERSION     DAOSWW010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68107_length_1597_cov_2.367704"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1588
                     /locus_tag="PK442_12235"
     CDS             143..1588
                     /locus_tag="PK442_12235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO88464.1"
                     /translation="MLSMAAGPLSVFVFSALLLTCVFLLYERRRLRKIVNEEALAKSA
                     LGESIVRFHMIVESSAAATYVFRGGRFVMVNAAMEAITGYSREELLRMNSLEHIHPDH
                     RPSALERVAARERGEAVSTRYELKLLTKTGEVRWVDFTARTVLFDGVLCTVGSAFDIT
                     GRKRVEDALRESEAKYRFLTESMKDVIWTLDAETLMFVYVSPSVERLRGYTPEEIMTE
                     PLDAALTPESAALVHRLIDQETEKFKMREERGDAVFHTEEVEQPRKDGTTVWTEVVTV
                     YWRNPQNGRIEIHGVTRDISERKEVEAKMAHMAQHDPLTGLPNLALFSDRLERALAAS
                     RRSGIPTALMFLDLDKFKPVNDTYGHAIGDVLLQQAAERMKSALRESDTVGRIGGDEF
                     VVLLPAVERVEDALHVAEKLLEAFRKPFFIEGRSVDVSCSIGIAIHPEDGRDEIELAK
                     NADTAMYWAKKDGGDCVRMFRTASDVSAAVL"
BASE COUNT          334 a          425 c          505 g          333 t
ORIGIN      
        1 ccgctttctg cgtttcctgg gaaaagttcg ttcatgagaa aacatgagaa aaaaggagcg
       61 acggaggaag ctcgatatgc tatccttaaa cggttacgga aattatgaag ccgttttccc
      121 tggtttcgcg ggaggaaaat atatgctctc aatggccgct ggtcctcttt cagtttttgt
      181 tttttctgcg ctgcttctta cctgtgtctt tttgctgtat gaaagacgtc gtctgcggaa
      241 gatcgtcaac gaagaggcgc ttgcgaagag cgctctcgga gagagtatcg tccgtttcca
      301 catgatcgtc gagagttccg ccgccgcgac gtatgttttt cgcggagggc gttttgtcat
      361 ggtgaacgcc gcgatggagg caatcaccgg ctactcccgc gaggagctgt tgaggatgaa
      421 ttctctcgaa cacatccacc ccgatcatcg tccttcggcg ctggagcggg tcgctgcccg
      481 ggagcgcggg gaagctgttt ccacacgcta cgaactgaag ctgctcacga agacaggtga
      541 agtgcggtgg gtcgatttca ccgcgagaac cgttctcttc gacggtgttc tctgcaccgt
      601 aggaagcgcc ttcgacatta cgggacgcaa gagggtggag gatgcgctca gggagagcga
      661 ggcgaaatac agatttctca cggagagcat gaaggacgtc atctggacgc tcgacgcgga
      721 gacgcttatg tttgtgtatg tcagcccctc cgttgagcgg cttcgaggat atacgcccga
      781 ggagatcatg accgaacctc tcgacgccgc tctgacgccg gagtcggcgg ccttggtcca
      841 caggctcatc gatcaagaaa cggagaagtt caaaatgagg gaagaacgcg gagacgcggt
      901 gtttcatacc gaggaggtcg aacagccccg caaggacgga acgactgtgt ggacggaggt
      961 cgtgaccgtc tactggcgca acccgcagaa cgggcgaatc gagattcacg gtgtgacccg
     1021 cgatatctcc gagcgtaagg aggtcgaggc gaagatggcc catatggcgc agcatgaccc
     1081 gctcaccggt ctccccaacc tggcgctctt ttccgaccgg ctggagcgtg cgctggccgc
     1141 gtccagaaga agcggaatcc cgacggcgct gatgttcctc gaccttgaca agttcaagcc
     1201 ggtgaacgat acgtacgggc acgcgatcgg cgatgtgctg cttcaacagg cggccgaacg
     1261 catgaaatcg gcgttgcgcg aatccgacac ggtggggcgg atcggcggcg acgagttcgt
     1321 cgtgctgctc cctgcggtgg agcgggtgga ggatgcgctc cacgtggcgg agaagctctt
     1381 ggaagccttt cgcaaaccgt tcttcatcga agggcgttcc gtcgacgtct cgtgcagcat
     1441 cggcatcgcc attcatccgg aggacggtcg cgacgagatc gagctcgcca agaacgctga
     1501 cactgccatg tactgggcga agaaagatgg gggagactgt gttcgcatgt tccggaccgc
     1561 ttccgacgtt tcggctgcgg tcctctgacg atacgat
//
LOCUS       DAOSWW010000711         1596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68147_length_1596_cov_3.297859, whole genome shotgun sequence.
ACCESSION   DAOSWW010000711 DAOSWW010000000
VERSION     DAOSWW010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68147_length_1596_cov_3.297859"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PK442_12240"
     CDS             complement(<1..476)
                     /locus_tag="PK442_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO88465.1"
                     /translation="MLLFELDERTPQSKSWQIVKRLLEMMKNGALTPGDRLPSTRKLA
                     ELLRVHRSTVALAYQTLWSLGYVELRRGTAPVVRCRVRVAEPGDREPLSAIDWSTVAS
                     PASEDVYRLFLARNVRREHTPDLQCISFEFIKMDPRLFPAERFRASLGRVFRDEGLS"
     gene            complement(572..1219)
                     /locus_tag="PK442_12245"
     CDS             complement(572..1219)
                     /locus_tag="PK442_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88466.1"
                     /translation="MLKVRSIFYCLEYGGALPALPIRRSLFLFFAVFCFAAFAASASA
                     RTVSPQADGRNAETVPFSSIREAYRQKAFDLWKRAADLQIGGRYEEALEKYREGLGYH
                     EDPRVREHVWKLEETLAARRGSENARKQAARRAAAELWKEAAALQQAGWFEDALQKYK
                     EGLELSDDQRVREHVKKLEEYIERLRHRGLLPVLVFMRERTGDALFKTPSSGVFL"
     gene            complement(1339..>1596)
                     /locus_tag="PK442_12250"
     CDS             complement(1339..>1596)
                     /locus_tag="PK442_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOO88467.1"
                     /translation="PPLALRLFRHREHVAEHSFIELLVAIAAGQLITSVLMVPYFLNM
                     LFGVPFTATLIVRLQTQLLQIPLFVMCIKLLARRLSFIVAG"
BASE COUNT          379 a          483 c          412 g          322 t
ORIGIN      
        1 gaaagtccct catccctgaa gactcttccc agagacgcgc ggaaccgttc ggcgggaaaa
       61 aggcgcggat ccatcttgat gaactcaaag gagatgcact gcaagtcagg agtgtgctca
      121 cggcgaacat tccgggcaag aaaaaggcgg tacacatctt cggaggcggg agatgcaaca
      181 gtcgaccagt cgatcgcgct cagaggttcc ctatcgcccg gctcagcaac ccgaacacgg
      241 caacgaacca ccggagccgt tcctctccgg agttcgacat acccgagaga ccagagcgtc
      301 tggtaggcta aggcgactgt ggaacggtgc acccgaagca gctccgccag tttcctcgtt
      361 gaagggagtc tgtcaccggg ggtcaacgca ccgtttttca tcatttcaag aagacgcttc
      421 acgatctgcc agctttttga ctgcggcgtg cgctcatcca actcaaaaag gagcatgggc
      481 tccctccttt ccggagatct acgcgcacta cactacaccg catgtggccc cttcacaact
      541 gccacttttt agtttttttg acgtaccact tttacaaaaa aacgcccgat gagggcgttt
      601 taaaaagagc atctccagtt cgttctctca tgaaaaccag aaccggaagc agtcctcgat
      661 gccgaaggcg ttcgatgtac tcttcaagtt ttttcacatg ctcgcgcacc cgctggtcgt
      721 cggataattc cagcccctcc ttgtactttt gaagcgcatc ctcaaaccac cccgcctgct
      781 gaagcgcagc ggcctccttc cagagctccg ccgccgccct gcgcgcggct tgttttctcg
      841 cgttctcact gcctcttctc gccgcaagcg tctcctcaag cttccagacg tgctctctca
      901 cccgtggatc ttcatggtat ccaagccctt cccgatactt ctcaagcgct tcttcgtacc
      961 gtccgccgat ctgcaagtcg gcggcccgct tccagagatc aaacgccttc tgacggtagg
     1021 cctcgcgaat cgaggagaag ggaacggttt ccgcgttacg cccgtcagcc tgcggagaaa
     1081 cagtcctggc tgacgcagac gccgcgaagg ccgcgaaaca aaacactgca aaaaagagga
     1141 acaaacttct tcggattggc agcgccggca gagcgccgcc atactccaaa cagtaaaaga
     1201 tactgcgcac tttcaacatt ctttctcccc tcctcctccg gtcgacgacg tcgcattgag
     1261 gaaccgacga aaaaacgcga tcaaaaaaag acgctcttct cgggacgttt cagaagggcg
     1321 tctttacttt ttcattggtt acccggcgac gatgaacgag agacggcgag cgagcagttt
     1381 gatgcacatg acgaagagcg gaatctggag cagctgcgtc tggaggcgca caatcagcgt
     1441 cgctgtgaac ggaacgccga agagcatatt cagaaaatac ggaaccataa gcaccgaggt
     1501 gataagctgt cctgccgcga tggcaaccag cagctcaatg aaggagtgtt cggcgacgtg
     1561 ttcccgatgc cggaagagcc tgagcgccag aggagg
//
LOCUS       DAOSWW010000712         1596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68173_length_1596_cov_2.289422, whole genome shotgun sequence.
ACCESSION   DAOSWW010000712 DAOSWW010000000
VERSION     DAOSWW010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68173_length_1596_cov_2.289422"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            704..>1596
                     /locus_tag="PK442_12255"
     CDS             704..>1596
                     /locus_tag="PK442_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HOO88468.1"
                     /translation="MRIVSDPIWLEEAICRTLGARGATTPGIDGMTRAHVENNIVELA
                     RTIGKELRTGTYEPQPVRRVNIRKANGKLRPLGIPTLKDRIVQRAMLMAMDPIWESDF
                     HNRSYGFRPCRSVHQAIRTVKVQLQDFGRGNGPRWIIEGDLTSYFDTIHHRLLMKAVR
                     RRIRDRRFLNLLWKFLKAGHVDKRLFHAAHDGVPQGGVISPLLGNIMLNEFDTYLENR
                     YLSTKTRRARESWNDSVKRKRPKAIEEGREWKPSVSYCRYADDFLISVKGTRAQAEEI
                     REECRKYLHNDLRLVLNMEKTR"
BASE COUNT          422 a          433 c          503 g          238 t
ORIGIN      
        1 tacccgttac ccatatcaca gcagaaagga gaagaatacg gcaagcggaa acaccgggga
       61 aacggggctg cccgcgctgc ggcgggcatg agtccgggcg gcagtgaccc ttcgcccgcc
      121 gaaggggtga tgtagcccgc cgacaaaggg gactgaggcg agaccgttac gccgagatgg
      181 tcttcgagac tgagcgtcga aaggtcgagg aacacgaacc gattgattcg catccgaggg
      241 tcgaatcccc accgcatcct acacggtctg acaggatgca tgcgggggga ggtacccggc
      301 gacgggagga agcctccgaa gccatctgct tattccttgc tggcagatgg tggagcgacg
      361 gggagaacgc agccagaccg tcgactccgc atcgacttgc ggcacgcaag ggcaaggact
      421 gcgatcggcg gcctcatcga cgcgcttgag ttcgcgttgc gtgaaacggg gaagcttcgc
      481 acggccgatg ggaggcggga gctccgggcc gcgcggggtg gcggagcacc cgtagtagtc
      541 cgagacgggg aaagcccgtt acatggcgaa ggggtgcagc ttggcgtatt tacctgataa
      601 actatctgac caagcgaggt gaagacctct gataatcaga gaaatacagc gtaaattagc
      661 gacatggtcc gaagagaaca aggcgagaag gttctaccga ctgttgagaa tcgtctccga
      721 cccgatatgg ctggaagagg cgatatgcag aaccctcgga gcccggggag cgacaacgcc
      781 gggaatcgac gggatgacga gagcccacgt ggagaacaac atcgtggaac tcgcgcgaac
      841 catcgggaaa gaactcagaa cgggcacgta cgaaccgcag cccgtgaggc gcgtcaacat
      901 caggaaggcc aacggcaaac tcagaccgct cgggataccg acgctcaagg acagaatagt
      961 gcagcgagca atgctcatgg cgatggaccc catatgggag agcgacttcc acaaccggtc
     1021 ctacggattc aggccctgca gaagcgtgca tcaggccata cgaaccgtga aggttcaact
     1081 tcaggacttc ggaagaggaa atggcccccg atggataatc gaaggggatc tgacgagcta
     1141 cttcgacacc atacatcaca gactgctcat gaaggccgtg aggaggagaa tccgggacag
     1201 gcgctttctg aacctgctct ggaagttcct caaggcggga cacgtcgaca aacggctctt
     1261 ccacgccgcg cacgacggcg tgcctcaagg cggagtcatc tctccgctgc tcggaaacat
     1321 catgctgaac gaattcgaca cgtaccttga aaaccgatat ctctcaacga agacgcgaag
     1381 agccagagaa agctggaacg acagcgtcaa gcggaagcgt cccaaagcaa tcgaagaagg
     1441 acgcgagtgg aagccttccg tgagctactg ccgatacgcc gacgactttc tgatatccgt
     1501 caaaggaaca cgagcgcagg cggaggagat acgcgaagag tgcaggaaat atctgcacaa
     1561 tgacctccga ctcgtcctga acatggaaaa gactcg
//
LOCUS       DAOSWW010000713         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68207_length_1595_cov_3.950000, whole genome shotgun sequence.
ACCESSION   DAOSWW010000713 DAOSWW010000000
VERSION     DAOSWW010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68207_length_1595_cov_3.950000"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /gene="minD"
                     /locus_tag="PK442_12260"
     CDS             <1..526
                     /gene="minD"
                     /locus_tag="PK442_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOO88469.1"
                     /translation="KDAVSSEQMEELCNLLRQEFEYILIDSPAGIESGFRNAAVGADE
                     ALVIATPEVPAVRDADRIIGLLESMGKAPIRLIVNRLRAQMVRDGEMLDVSDVLDILA
                     IELIGVVPEDDSVVKSANRGEPLTFSDVSPAALAFQRIAMRLTGKEVPFPKLDEENGR
                     GLFAGIRKLFGMKN"
     gene            546..830
                     /gene="minE"
                     /locus_tag="PK442_12265"
     CDS             546..830
                     /gene="minE"
                     /locus_tag="PK442_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOO88470.1"
                     /translation="MDFLKKFFSSSGSKSRAKERLQIVLIHDRADISPEMLDNLRQDM
                     IAVITRYMDIDTSKIEMDFDRADRSVAFVANIPVIRIKRGVAAAREGERG"
     gene            894..>1595
                     /locus_tag="PK442_12270"
     CDS             894..>1595
                     /locus_tag="PK442_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOO88471.1"
                     /translation="MEEKKLSLGESVAGLDKAQLFSVGALFLLGVLSIFSAGAGFEGR
                     GAGFATRQLLWGAVSIAAFLLTLKIGYEKFLREAYLLYGLSLVLLLSVMALGLTVKGS
                     QRWIDLGFFRLQPTEFVKITLVLVLAKHLCRYPPLNFLSFLGGLAVPALSGILVLIQP
                     DAGSAIVYGVISFVAIVVAGAPWRYPAGLLAIFTSLLPFAWRFLREYQKMRIRVFLDP
                     WIDPLGAGYNVIQSRI"
BASE COUNT          296 a          423 c          512 g          364 t
ORIGIN      
        1 caaggacgcc gtctcctccg aacagatgga ggaactgtgc aaccttctcc ggcaggagtt
       61 cgagtatatc ttgatcgaca gtccggcggg gatcgagagc ggtttccgga acgctgccgt
      121 gggcgcggac gaggcgcttg ttatcgccac gccggaggtg ccggctgtgc gtgacgccga
      181 caggatcatc ggccttcttg aatcgatggg aaaagcgccg atccgtctga tcgtcaaccg
      241 cctccgcgcg cagatggtcc gcgacggcga gatgctcgac gtttccgacg tactcgacat
      301 actcgcgatc gagcttatcg gtgttgttcc cgaggacgac agcgtcgtga agtcggccaa
      361 ccgcggcgag cctcttacct tctccgacgt ctctccggcc gccctcgcat ttcagaggat
      421 cgccatgcgt ctgacgggga aagaggtccc ttttccgaaa ctcgacgagg aaaacggcag
      481 gggacttttc gccggtatcc gcaagctctt cgggatgaag aactgagagg agaggagggg
      541 tggcgatgga ttttctcaag aaatttttca gctcttccgg gtcgaaatca agggcaaaag
      601 agcgtctcca gatcgtgctt attcacgacc gggcggatat ctctcccgag atgctcgata
      661 atctccggca ggatatgatt gccgtgatca cgaggtatat ggacatcgat acatcgaaga
      721 tagagatgga cttcgaccgt gcggacagat ccgtggcgtt cgtggcgaac atccctgtga
      781 tccgcatcaa acgaggggtt gcggcggcga gagagggaga acggggctag cgtgtccccg
      841 cggaggagaa cgccgtcgga agcggagacg ccgcgtacga gggagaactt gtaatggaag
      901 aaaaaaaact ttctcttggc gagagtgtcg caggcctgga caaggcgcag cttttttccg
      961 taggcgcgct ctttctgctc ggggtgctct ccatattcag cgccggcgcc ggttttgagg
     1021 ggcggggagc ggggtttgcg actcgccagc tcctctgggg cgcagtgtcg attgcggctt
     1081 ttttgctcac tctcaagata gggtacgaaa aatttctgcg ggaggcgtat ctcctgtacg
     1141 gcctttcgct ggtgctcttg ttgagcgtca tggcgctggg gctcaccgtg aaggggtcgc
     1201 agcggtggat tgatctgggt ttcttcaggt tgcaaccgac ggagttcgtc aagataacgc
     1261 tggttctggt gcttgccaaa catctgtgca ggtacccccc gttgaacttt ctctcttttc
     1321 tgggggggct cgccgtgccc gcgctttcgg ggatcctggt gctgattcaa ccggatgccg
     1381 gaagcgcgat cgtctacggc gtgatctcct ttgttgcgat tgtcgtcgcc ggagccccat
     1441 ggaggtatcc ggcggggctg ttggccatct tcacgtcgct tcttccgttc gcatggcgtt
     1501 ttttgcggga gtatcagaaa atgcggatac gcgtctttct tgatccgtgg atcgacccgc
     1561 tcggcgccgg gtacaacgtt atccagtcga ggata
//
LOCUS       DAOSWW010000714         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68218_length_1595_cov_3.330519, whole genome shotgun sequence.
ACCESSION   DAOSWW010000714 DAOSWW010000000
VERSION     DAOSWW010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68218_length_1595_cov_3.330519"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(815..>1595)
                     /locus_tag="PK442_12275"
     CDS             complement(815..>1595)
                     /locus_tag="PK442_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88472.1"
                     /translation="VYIDGPKQIPDILARVPTQSGREVWLVLHLEIQGPGGGDLPERM
                     FYYNSTLRVKYLKEKGDVADAVSCAILTAKRPSLEEERYLRESYGNRMFYEYPTLKLC
                     ELGADELEESENPFDWALCAGKRALESGRNERLKLVYLKDMIVKLDYKEWTHDEKLLL
                     LRLIDSLLHPKSPELRAEYGEFREELRKEGKAMLLSIVEERAQEEGREIGKSEGILIG
                     REETKREAALRMLRKGMSVSLAAECVDLPEEEVARLAEEAR"
BASE COUNT          303 a          519 c          392 g          381 t
ORIGIN      
        1 cgaaaatccg caccggttac cctacaatgc gtttttcgcc ctcaccccga tgtgctccgc
       61 tgattttata agctgcttgt cagcggtaat cagaacagcc tctttttgca gtgccgttgc
      121 gcatattatc gcgtccggca gtttcagatt gctgttttat ctgacgtaca ccgggtttct
      181 tactattttc tcatttatat acagcacttc aagagtcgaa agcgaccggc gggtcgctca
      241 gtctgatgcc cgacagtgaa gaactcgtca tcatgctcgc tgcttttcta ttctatacga
      301 tgctctcacg agaaaagaga gggcgacatt gcgccgccct ctctttgtcc gtgcctcgcc
      361 aatccgaacc gaaacgcgcc aagcctaacc atttcccgcc gaacgttgcc atatcgggcc
      421 cagccctgcc gagttccgca acctccttaa ttgaggcgcg gcaatcatat tctctacttc
      481 gtatgctccg gcatcaatcg tactcattga ggggatatcg tgagatacga ccgaagtgtt
      541 ctcggagagc aggcgaaaaa atcgggcttc gtcagagata cattcgagaa gatggtccgt
      601 ttgaccgacg tccttcgttt catccattcg gaagagtctc gttctcctct cccgtccgga
      661 gtctggaaga agaacttatt gacgatcacg cgccgatact tgccaagccg gaacgcgctc
      721 cggagtctcc cggcgagtcc ggagcactcg tcccgcaatg aatttagacg gggggagctc
      781 gtttgcgagc acctcccgtc gcgagtcgaa cggatcagcg cgcctcttcg gcgaggcgag
      841 caacctcctc ctcgggaaga tcgacgcact ccgccgccag agagacgctc atccccttgc
      901 gcagcatcct gagagcggcc tcgcgctttg tctcctccct gccgatcagg attccctcgc
      961 tcttgcctat ctccctgcct tcctcctgcg ctctttcctc gactatgctc aacagcatgg
     1021 ccttcccctc cttcctcagt tcctctctga attctccata ctccgccctc agctcggggc
     1081 tcttcgggtg cagcaggctg tcgatcagac ggaggagcag cagcttctcg tcatgcgtcc
     1141 actccttgta gtcgagcttc acgatcatgt ccttcagata gaccagcttg agccgctcgt
     1201 tccgtccgct ctccagcgcc cgcttcccgg cgcagagcgc ccagtcgaac ggattctcgc
     1261 tctcctcaag ctcgtcggcg ccaagctcgc acagcttgag cgtcgggtac tcgtagaaca
     1321 tcctgtttcc gtagctctca cgcagataac gctcctcctc caaggagggg cgcttcgccg
     1381 tcaggatggc gcaggagacc gcgtcggcaa catcgccttt ttccttcagg tacttcaccc
     1441 gaagcgtcga attataatag aacattctct ccggaaggtc gccgcctccg gggccttgta
     1501 tctccaagtg gaggaccagc cagacctccc gccccgactg tgtcggaacg cgggccagaa
     1561 tatcggggat ttgcttcggt ccatcgatgt agacc
//
LOCUS       DAOSWW010000715         1593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68367_length_1593_cov_3.819896, whole genome shotgun sequence.
ACCESSION   DAOSWW010000715 DAOSWW010000000
VERSION     DAOSWW010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68367_length_1593_cov_3.819896"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /gene="istA"
                     /locus_tag="PK442_12280"
     CDS             complement(<1..1361)
                     /gene="istA"
                     /locus_tag="PK442_12280"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOO88473.1"
                     /translation="MTTQYREILRLASLGLSGRSIAGSVSCSRNTVSEVLARAAKKGL
                     VWPLPLEVGEGDLRKLLFPEKEKDASSRTPDCEAIHRELAKSGVTMTLLWDEYCRKCR
                     EGGEIPYQYAQFCKIYRKYAAVSKATMHIERKPGERMEVDWAGKGMKIRDSVTGEERG
                     MHLFVAVLPASQYAYVEAFPDMGMESWIRGHTNAFTHFGGVPRMVVPDNLKTGVEKAD
                     WYSPVVNRTYHEMAEHYGTAIVPARVRHPKDKASVEGTVGHITTWIAAALRNRTFFTV
                     GELNLEIRKKLEELNERPFQKRPGSRLSVFLEEERDFLFPLPASSYEIAVWKTATVSF
                     NYHVEVERNFYSVCHEYIKQKVDVRVTSRMVEIFYNGVRICSHPRVNGAPGQYRTVPE
                     HMPESHRQSAEWSGERFVSWASSIGPNTEAVVRSVLAGCKVEQQGFRTCMGILKMADK
                     YSV"
BASE COUNT          282 a          534 c          420 g          357 t
ORIGIN      
        1 tcgacggagt acttgtccgc catcttcagg atgcccatgc atgtccgaaa accctgctgt
       61 tccaccttgc atccggcgag cacgcttcgg acgaccgcct ccgtgttcgg tccgatgctc
      121 gaagcccacg aaacgaagcg ttctccgctc cactccgcgc tttgacggtg gctttccggc
      181 atgtgctccg gcaccgtccg gtactgtccc ggagcgccgt tcaccctggg gtgggagcat
      241 atgcgaaccc cgttgtagaa gatctccacc atcctgctcg tgacgcggac gtcgaccttt
      301 tgcttgatgt actcgtggca cacgctgtag aaattcctct ccacctcgac gtggtagttg
      361 aaggagacgg tcgccgtctt ccacacggcg atttcgtagg aactcgccgg cagggggaaa
      421 aggaaatccc gctcttcctc cagaaagacg ctcagtctgc ttccggggcg cttctggaag
      481 gggcgctcgt tcaattcctc aagcttcttc cgaatctcga ggttgagttc gcccacagtg
      541 aagaaggttc tgttcctcag cgccgccgcg atccaggtgg tgatgtggcc gaccgttccc
      601 tcgacgctcg ccttgtcctt gggatgccgt actctggccg gaacgatcgc cgttccgtag
      661 tgctcggcca tttcgtggta cgttctgttg acgaccggag aataccagtc cgccttctcc
      721 acgccggtct tgagattgtc cggcaccacc atgcggggaa ctccgccgaa gtgcgtaaaa
      781 gcgttcgtat gcccgcgaat ccagctctcc atacccatgt ccgggaaggc ttccacgtaa
      841 gcgtactggc tcgccggaag caccgcgacg aagagatgca tccctctttc ttcgcctgtg
      901 acggagtcgc gaatcttcat ccctttcccc gcccagtcca cctccatgcg ttctcccggc
      961 ttcctctcga tatgcatggt cgccttggag actgcggcgt acttgcggta gatcttgcag
     1021 aactgtgcat actgatacgg aatctcgccc ccttcccgac acttccggca gtactcgtcc
     1081 cacagaagcg tcatcgtcac tcccgacttc gccagttccc ggtgaatggc ctcgcagtcc
     1141 ggcgtccggg acgacgcatc tttctccttc tcgggaaaga gcagctttct caagtccccc
     1201 tcgccgactt ccagcggcag aggccatacc agtcctttct tcgccgcccg agccagcact
     1261 tccgaaacgg tgttgcggga gcaggaaacg ctccccgcga tgctcctccc gctcagcccg
     1321 agcgaggcca gccgcagaat ctccctgtac tgcgtcgtca taccactaaa acctccctga
     1381 ttcgaattcg cacgcatccg cgcacattcg aattgtaggg aaaacggctc tcgggcagga
     1441 gaaatcccgg ctctatttct ggatatacgt ggctctattc cgggagaacc gtggctctct
     1501 ttttggagac aagcggctct cccgctgtgt gatattcaga aagacttccg cgcgctccca
     1561 ttcgcccccg gcggccatct tcccgagcga ggg
//
LOCUS       DAOSWW010000716         1593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68382_length_1593_cov_2.904421, whole genome shotgun sequence.
ACCESSION   DAOSWW010000716 DAOSWW010000000
VERSION     DAOSWW010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68382_length_1593_cov_2.904421"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PK442_12285"
     CDS             complement(<1..857)
                     /locus_tag="PK442_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88474.1"
                     /translation="MGERRQGAGRRMAAAPSAETTPKGKRTRRMSGSGFQEEEMLDDV
                     SLDEGVDSVDPTDEEDDEFFEPSPLGSADGRARPIWLCAGDALLWCNPRFGYNIQFRE
                     TLRRLLETFLGNDGLRSMGKPFDSPDIEERLFLLLGFFREVWLRNLFKNEESDGKSLS
                     DEYAPPEEEDSGEESLSDEETPPEDEEYDGEARPEKTRNKSVSLQSRLRSLNSVGMRL
                     PGGEVLPLSLFLAGKGERTESVPRRLERRWIRDVFRERGLDAGFKWKDVKEWLPSEYE
                     HFVESLRSL"
     gene            complement(870..>1593)
                     /locus_tag="PK442_12290"
     CDS             complement(870..>1593)
                     /locus_tag="PK442_12290"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88475.1"
                     /translation="PVGMSRCLNERDLQEIVGYWRGEGDVSCEVLRTHLRERDGVLEI
                     RSPLSCSAVSPCVCSLCCGADLARKPFDDPAPLPLGARVGLTAAQAIGERGTQLAMKR
                     FHQVGDPKNTANNDAASNGEETTLPGGVPAVLRALLVDAGADSEKTQEERFVSLLENV
                     LASGKGEKEKKKAEKDLPQSLIHFEIALRPEKGLYSEAESRIGNAFLSRLAFEGAGEV
                     LKEIDKECIDDLTSTKSRLLWS"
BASE COUNT          337 a          522 c          364 g          370 t
ORIGIN      
        1 aaaagagatc gaagactttc gacgaaatgt tcgtattccg aaggcagcca ctccttcaca
       61 tctttccact taaagcccgc atcgagcccc cgttcccgga agacatcgcg tatccatcgc
      121 cgctcaagcc tccgtggtac gctttcagtt ctttcccctt tccccgcgag aaaaagagaa
      181 agcggcagca cttctcctcc cggcagccgc ataccgacgg aattcagcga acggagtctc
      241 gactgaagac tcacgctttt atttctggtc ttttcgggcc ttgcctcccc gtcgtactcc
      301 tcatcttccg gaggagtctc ttcatcggaa aggctttcct cgccggaatc ctcttcttcc
      361 ggaggggcgt attcgtcaga aaggcttttc ccgtcggatt cctcgttttt gaaaagattt
      421 ctcagccaca cctcccggaa aaagcccaaa agaagaaaaa gcctttcctc gatatccggg
      481 gaatcgaacg gtttccccat gctccgcagg ccgtcgtttc ctaaaaatgt ttccagaagc
      541 cttcgcaatg tttcccgaaa ctggatatta taaccgaagc gcggattgca ccatagaaga
      601 gcatctccgg cgcacagcca gatcggcctc gctctcccgt cggcggaacc gagaggagac
      661 ggttcaaaaa actcgtcgtc ctcttcatcc gtgggatcaa cactatctac tccttcgtcg
      721 agagaaacgt catcaagcat ttcctcttcc tgaaagccgc taccgctcat tctccgcgtt
      781 cttttcccct tcggcgtcgt ttccgccgaa ggggctgcgg ccattcttct tcccgctccc
      841 tgtctgcgtt cacccaacga cagcaccccc taactccaga gcagacgact ctttgtgctc
      901 gtaagatcgt cgatacactc tttgtcgatc tccttcagca cttcgcccgc tccttcaaaa
      961 gccaatctgg agagaaacgc gttcccgatg cggctctcgg cttcgctgta aagccccttt
     1021 tccggccgca gcgcaatctc gaagtgaata aggctctgcg gaagatcctt ctccgccttc
     1081 tttttttcct tttcgccttt tccggaggcc agcacatttt ctaacaagga aacgaagcgt
     1141 tcttcctgag ttttttcgga atcggcgccg gcatccacta aaagagcgcg caaaacagcc
     1201 gggacgccgc cggggagcgt cgtttcttcc ccgttacttg ccgcatcatt gttcgccgta
     1261 ttcttcgggt cgcccacctg atggaagcgc ttcatcgcaa gctgggttcc tcgttcccct
     1321 atcgcttgtg ccgcagtcag tcccactcgt gcgccgagcg gcagaggagc cgggtcgtcg
     1381 aagggcttcc ttgcgagatc cgctccgcaa cacaaagagc acacacacgg ggatacagcg
     1441 ctgcaggaaa gaggagaacg aatttccagc acgccgtcac gttcccggag atgcgtccgc
     1501 aacacctcgc aggaaacatc accctcaccc ctccaatacc cgacgatctc ctgaagatcc
     1561 cgctcgttca agcagcgcga catcccgaca ggg
//
LOCUS       DAOSWW010000717         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68447_length_1592_cov_3.396877, whole genome shotgun sequence.
ACCESSION   DAOSWW010000717 DAOSWW010000000
VERSION     DAOSWW010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68447_length_1592_cov_3.396877"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PK442_12295"
     CDS             complement(<1..225)
                     /locus_tag="PK442_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88476.1"
                     /translation="MAKKKRPSLKDYLSTGQVIAEEVQEQSVSLEEAPAAPPAGEPSA
                     PEKKPAKARKPRSSAAKIGAAKKKSPVPDES"
     gene            complement(218..985)
                     /locus_tag="PK442_12300"
     CDS             complement(218..985)
                     /locus_tag="PK442_12300"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOO88477.1"
                     /translation="MKIVGFCNLKGGVGKTTACQNLAVALVRRGLKVAALDLDPQSNL
                     TAGFGISIPEDRPYIYDFLMGDVSFGDLKTVREGVDVAPSTLDLAVVEMQLESQPGRD
                     TLLKDALASSDLSEYDVVFCDSPPQLGIFTRNVLAACGEILVPLESEFYSLAGLRLLS
                     QTVELYKKRLNRGLQISGILLTRHNPKIIMNREVEKEVRSHFGDTVFRRYIRQNISLV
                     EASGAGLSVFGYDEESNGARDYGVLADELLERWKKNG"
     gene            1160..1405
                     /locus_tag="PK442_12305"
     CDS             1160..1405
                     /locus_tag="PK442_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88478.1"
                     /translation="MTTASGSRSPRRRHFDGIANVKLLREITRHLFAETTDLLFLFTL
                     EGKLREISRSASDELGYPEEDALSLNFFADLLAVPLR"
     gene            1408..>1592
                     /locus_tag="PK442_12310"
     CDS             1408..>1592
                     /locus_tag="PK442_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88479.1"
                     /translation="MEQLCKSLLDGQDVTLAGTVRRRDGSGCAVQLTVFRPGEGREAL
                     FLCSAKKPEEEKPPRDTG"
BASE COUNT          322 a          476 c          447 g          347 t
ORIGIN      
        1 gctttcatcc ggaacgggag attttttctt cgccgcgccg atcttcgccg cgctgctccg
       61 cggttttcgc gccttggcgg gtttcttttc cggcgcggac ggctcgccgg ccggaggagc
      121 cgcaggcgcc tcttcgagcg aaacagactg ttcctgtacc tcctcggcaa tcacctgccc
      181 ggtcgacagg tagtccttca agctcggtct ctttttctta gccatttttc ttccacctct
      241 ccagaagctc gtcggccagt acgccgtagt ctctggcgcc gttgctctcc tcgtcgtatc
      301 cgaagacgga gagccccgct ccgctggcct ccacgagact gatgttctgc cggatgtaac
      361 gccggaagac cgtgtcgccg aaatgactgc ggacttcttt ttccacttcg cggttcatga
      421 tgattttcgg gttgtgcctc gtcagaagta ttccgctgat ttgaagcccc cggttcaggc
      481 gctttttgta gagttccacc gtctgcgaga gaagacggag tcctgcgagg ctgtaaaact
      541 cgctctcaag cgggacaagt atctccccgc aggcggcaag gacgttccgt gtgaaaatgc
      601 cgagttgcgg ggggctgtcg cagaagacga cgtcgtactc cgaaaggtcg gacgaggcga
      661 gggcgtcctt cagcagcgta tcgcgtcccg gctggctttc gagctgcatc tccaccacag
      721 cgaggtccag cgtgctcggc gccacatcca ctccttcgcg gacagttttc aggtccccga
      781 aagaaacgtc tcccataagg aagtcgtaga tatacggacg gtcctccggg atagagatgc
      841 cgaagccggc ggtgagattg ctctgcggat cgaggtcgag cgcggctact ttcagtcctc
      901 tgcggacaag agccaccgca aggttctggc acgcggtggt cttgccgaca ccgcctttga
      961 gattgcaaaa accgacgatt ttcatgaaga atccccccgg acaaatgtgc gatcaaagca
     1021 tccagtcgag tatataacat aacctcgcaa tatagcatag caagaaacgg ctgttgcgaa
     1081 aagacgtctt ttttcgcagt gtagagtata attggtcaac tacatgcgat tcttcgccat
     1141 ctaaaaggag gcggcactta tgacgacagc gagcggctcc cgttccccgc gccggcggca
     1201 tttcgacggg atcgccaacg tgaaactcct gcgtgagatt acacggcatc ttttcgcgga
     1261 gacaaccgac ctgttattcc tcttcaccct cgaagggaag ctgcgcgaga tcagccgatc
     1321 ggcaagcgac gagttgggat accccgaaga ggacgcgctc tcgctgaatt ttttcgccga
     1381 tcttctcgcc gttcctctcc ggtaaaaatg gaacaactct gtaaatccct cctcgacggg
     1441 caggatgtta ccctcgcagg aacagtccgg cggcgcgacg gaagcggctg cgcagtgcag
     1501 ctcacggtct tccgcccggg cgagggaaga gaagcgctct ttctttgttc ggcgaaaaag
     1561 cccgaagaag aaaagccccc ccgcgacacc gg
//
LOCUS       DAOSWW010000718         1585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_68948_length_1585_cov_3.158170, whole genome shotgun sequence.
ACCESSION   DAOSWW010000718 DAOSWW010000000
VERSION     DAOSWW010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68948_length_1585_cov_3.158170"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1125)
                     /locus_tag="PK442_12315"
     CDS             complement(196..1125)
                     /locus_tag="PK442_12315"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOO88480.1"
                     /translation="MGYKLHISPSDDGRRLDKTIRSIWPALPLSVVMKGIRTGAVRLD
                     GKKAACDVRITAGQELYVPWEGPAEREEGFRYRRTLPILFEDERLLAVNKPENLLVQP
                     DRKDQDNVLDRVKYMRSLEGAEERAYAVHRLDRNTTGVLLVALSGVSLRILQDAFRAR
                     EVRKTYLAVVSGVPPAKGEIDAPLLKDPASNTVKVDPSGKEALTRYRLLAAGGDVSLV
                     EIDLVTGRSHQARVHMASIGHSILGDIRYGSENVNAKWRKRGIRRPLLHSYQVVFGSL
                     LGELESLSGMRIAAPLPEDMGELLRSRGWKPPV"
     gene            complement(1115..>1585)
                     /locus_tag="PK442_12320"
     CDS             complement(1115..>1585)
                     /locus_tag="PK442_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO88481.1"
                     /translation="IAVARTAKSLVEAGIAGGYEADVAVTLAGALLHDLGKMEAYRLV
                     PAPVMTVAGTFHDHIVIGYSMFESLAREFNLDERLFLAIGHIIVSHHGCREYGSPVLP
                     ATPEAMAVSAADEMDFRLFCWKHSVDQMEDGREISDFNPSAQRRFWKWELNNGV"
BASE COUNT          341 a          509 c          391 g          344 t
ORIGIN      
        1 ccaccggagg tttcccgtcc tctatacgga cgactctcct ggcatttcag ccgacaccga
       61 gtgtccgccg gattgcatag acttctcccc tcttccgcag cttttccaga atcttctctt
      121 ccgcaacgat ttttacatgt tccatgctct tccctccttc cctgacgaag tttgtccttc
      181 gcgggtagaa ccgggttata ccggaggttt ccagcccctc gaacgtagaa gctcccccat
      241 atcctccgga agaggagcag ctatccgcat gcctgaaagc gattcaagtt ctcccagaag
      301 gctcccgaag acaacctgat aactatgcag aagagggcgg cgaatccccc tcttcctcca
      361 cttcgcattg acattttcgc tgccgtagcg aatatctccg agaatcgaat gacctatcga
      421 cgccatgtgc acccgggcct ggtgagagcg ccccgtcacg aggtcgattt cgacgagaga
      481 aacgtcccct ccggctgcaa gaagtctgta gcgagtgagc gcctccttcc ccgaaggatc
      541 caccttaacg gtgttggaag cgggatcctt gagcaatggg gcgtcgatct ctccctttgc
      601 aggaggaacc cctgaaacta cggcgagata cgtctttcga acttcacgag cccggaaggc
      661 atcctggagt atacggagag agaccccgga gagcgccacc agaagcactc ccgtcgtgtt
      721 cctgtccagc cggtgcactg cgtaggctct ctcctcggcc ccttcaagac tccgcatgta
      781 cttcaccctg tcgagcacat tgtcctgatc cttccggtcc ggctggacaa gcaggttttc
      841 cggcttattc acagccagca agcgctcgtc ctcgaacaga atgggaagtg tgcgacgata
      901 gcggaaaccc tcttcccttt cggcaggccc ttcccaaggg acatacagct cctgccccgc
      961 cgttatcctg acgtcgcacg ccgccttttt cccgtccagc cggacagcgc ctgtacggat
     1021 gcctttcatt acaacgctga gcggcaacgc aggccagatc gaccggatcg tcttgtccag
     1081 gcgacgcccg tcatcagaag gtgaaatatg cagtttatac cccattgttc agctcccatt
     1141 tccagaaacg tctttgggcg gaaggattaa aatcggatat ttcccgtcca tcttccatct
     1201 gatcgacaga atgcttccag cagaagagcc tgaaatccat ctcgtcggcc gcgctcaccg
     1261 ccatggcttc cggagtcgcg ggaagcacag gagaaccgta ctccctgcat ccgtgatggc
     1321 tgacaatgat atgccctatg gccagaaaaa gccgttcatc caggttgaat tcacgggcga
     1381 gggattcaaa catgctgtac ccaatcacaa tatgatcgtg aaaggtcccc gccacggtca
     1441 ttacaggcgc aggaacaagg cggtacgcct ccatcttgcc aagatcatgg agcagcgctc
     1501 ccgccagcgt gacggcaacg tccgcttcat aaccgccggc tattcctgcc tcgacgagac
     1561 ttttcgccgt gcgcgcaaca gctat
//
LOCUS       DAOSWW010000719         1583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69079_length_1583_cov_4.101440, whole genome shotgun sequence.
ACCESSION   DAOSWW010000719 DAOSWW010000000
VERSION     DAOSWW010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69079_length_1583_cov_4.101440"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK442_12325"
     CDS             <1..332
                     /locus_tag="PK442_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88482.1"
                     /translation="KINSVGTTRPLPPLPAEDIVEEKVEETKEEGLKKVEKAKRAVET
                     ERLEIMDRHHETVKKSAETYDKIVKKRLMEKEIEERRLQQRELLAERNEEAERRRDEM
                     RKAAEKD"
     gene            348..1262
                     /locus_tag="PK442_12330"
     CDS             348..1262
                     /locus_tag="PK442_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO88483.1"
                     /translation="MKKRLPEALRASSVVFVMLFFLLFAGQPLHAASVEENGTYADFD
                     GLRVHYVDEGPRERPALVFIHGWSCDVSFWRFQTEELRDKFRVIAIDLPGFGLSDKPA
                     DRAYTLDFFAGAARAVIKAAGVEKPVLIGHSMGYPVTTKYLALSPGEVRAIVNVDGAY
                     FRSPSNPRTVEELKEMNAFLEGLLGPERPQFIGFFIESTFYGRTPQELRTEIMEKMAS
                     ADEYAANSSMKEFIVPENWEERSYDVPALAVYSAHEYVPPDHEAYLRTVFPNLTYEEW
                     PDTGHFLMMEAPERFNAVLVKFVEGLPE"
     gene            1393..>1583
                     /locus_tag="PK442_12335"
     CDS             1393..>1583
                     /locus_tag="PK442_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88484.1"
                     /translation="MENKTEKLILLPCFDSPEAAQACRRDLKIPREKAITLGTSAMEA
                     CDRGYYFDEQGAKVDWGQLV"
BASE COUNT          354 a          409 c          516 g          304 t
ORIGIN      
        1 tgaaaatcaa ttccgttggt acgacgcggc ctcttccccc tcttcccgcc gaggacattg
       61 tcgaagaaaa ggtcgaagag acgaaggaag aggggctgaa gaaggtcgag aaggcgaagc
      121 gcgcagtcga gacggagcgc cttgagatca tggacaggca tcacgagacg gtaaaaaaat
      181 ccgcggagac gtacgacaag atcgtaaaaa aaaggttgat ggagaaggag atcgaggagc
      241 gtcgccttca acagcgcgag ttgttggccg aacggaacga agaggccgag cggcggcgcg
      301 acgagatgcg gaaagccgcg gaaaaagact aaaacggagg ggtattggtg aaaaaacgtc
      361 ttcccgaagc gctgagagcg tcttcggtag tttttgtaat gttgtttttt ttgttgtttg
      421 caggacagcc gctgcatgcg gcgtccgtcg aagagaacgg gacgtacgcc gatttcgacg
      481 gactcagggt gcattatgtg gatgaagggc ccagggagcg ccccgcgctc gtcttcattc
      541 acggctggtc gtgcgacgtc tccttctggc ggtttcagac cgaggagctg cgcgacaagt
      601 ttcgcgtgat cgcgatcgac ctgccgggat tcgggctgag cgacaagccc gccgacaggg
      661 cgtacacgct cgatttcttt gcaggcgcgg cccgggcggt catcaaggcg gccggagttg
      721 agaaacccgt cctgataggg cacagcatgg ggtacccggt gacgacgaag tacctcgcgc
      781 tctctccggg ggaggtccgc gcgatcgtca acgtggacgg cgcgtacttc cggtccccgt
      841 cgaacccgcg gacggtcgag gagctgaagg agatgaacgc gttcctggag gggctgctcg
      901 gccccgagcg accgcagttc atcggtttct tcatcgaatc caccttctac ggcagaactc
      961 cgcaggagct gcggaccgag ataatggaga aaatggcatc cgccgacgaa tacgcggcga
     1021 acagctcgat gaaagagttc atcgtaccgg agaactggga agagcgctcc tacgacgttc
     1081 ctgccctggc ggtctactcc gcgcacgaat acgtcccgcc cgatcacgag gcatacctgc
     1141 ggacggtctt tccgaatctg acctacgaag agtggccgga tacgggccac ttcctgatga
     1201 tggaggcgcc ggagcgcttc aacgcggttc tggtgaagtt cgtggaagga ctgcccgagt
     1261 agagcgaagg aggaggggag attttccctc ctccttcttc gcattcccct ggatatgcgt
     1321 ttggttttag gatgtgaagt tcattagccg ttctgtttgt ggttccatga aagttgatct
     1381 gcgagggatg ctatggaaaa caaaaccgaa aaactgattc tgctgccctg tttcgactcc
     1441 ccggaagcgg cgcaggcgtg ccgccgggat ctgaagattc cccgcgagaa ggcgatcacc
     1501 cttggcactt ctgcgatgga ggcgtgcgac agagggtact attttgacga gcaaggagcg
     1561 aaggtggact ggggccaact cgt
//
LOCUS       DAOSWW010000720         1579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69371_length_1579_cov_4.524934, whole genome shotgun sequence.
ACCESSION   DAOSWW010000720 DAOSWW010000000
VERSION     DAOSWW010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69371_length_1579_cov_4.524934"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="PK442_12340"
     CDS             <1..116
                     /locus_tag="PK442_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOO88485.1"
                     /translation="VREIAELKKVDLNSYDVDAAMRMVEGTARSMGITIVD"
     gene            179..889
                     /gene="rplA"
                     /locus_tag="PK442_12345"
     CDS             179..889
                     /gene="rplA"
                     /locus_tag="PK442_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOO88486.1"
                     /translation="MAKLSKRLTAAHQKIEDGKQYTVKEAIALFKETATAKFDESMEV
                     HIRLGVDPRHADQQVRSTVVLPHGTGKTKRVLVLAMGEKVKEAEEAGADFVGGEELVG
                     KISGGWLDFDAVIATPDMMKSVGRLGKVLGPRGLMPSAKTGSVTFDIASAIKEIKAGR
                     VEFRVDKFAIVHNAIGKKSFSNEQLFDNAKTLIQAVLKARPASVKGTYVKSLNLSSSM
                     GPGIKIDVASAQKEVAGE"
     gene            1077..>1579
                     /gene="rplJ"
                     /locus_tag="PK442_12350"
     CDS             1077..>1579
                     /gene="rplJ"
                     /locus_tag="PK442_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOO88487.1"
                     /translation="MPATLKYEQVDELKGRLARTQAIFACEYRGMTVAQITALRAQVR
                     AAGGEMKVAKNTLMRIAMEQEGFIPFPDDMAFGPNVFALAYGDPVAVAKALRDFAKDR
                     TNKFFVLKGCVLGQTLLDATQIGALADLPSKEVLIGQVVRTIAAPIAGLLTVLSGPAR
                     GLVTCLN"
BASE COUNT          369 a          403 c          475 g          332 t
ORIGIN      
        1 aagtgcgcga gattgcggag ctgaaaaagg tcgatctgaa ttcctatgac gtcgacgccg
       61 caatgcgtat ggtcgaggga actgcgcgtt caatgggtat cactatcgtc gattgatgaa
      121 aagtggtttt gaatttgtgg gaggcctcaa ggctgtctga ccaccaggag ggggaaaaat
      181 ggcgaagttg agtaaaagac tgactgcagc acaccagaag atcgaggacg gaaaacagta
      241 caccgtgaaa gaggcgatcg cgctttttaa agagacggcg accgcgaagt tcgacgagag
      301 tatggaggtc catatccgtc tcggcgtaga tcctcgtcac gctgaccagc aggtccgcag
      361 cacggttgtg ctcccccatg ggacgggaaa gaccaagaga gttctcgttc ttgccatggg
      421 tgagaaagta aaagaagcgg aagaggccgg agctgatttt gtcggcggcg aagagcttgt
      481 gggcaaaatt tccggcggat ggcttgattt cgacgccgtc atcgcgaccc ccgacatgat
      541 gaaatcggtc ggacgcctcg gaaaggtgct tggtccccgc ggccttatgc ccagcgcaaa
      601 gaccggcagc gtgacgttcg atattgcgtc cgcgataaaa gagatcaagg ccggacgcgt
      661 cgaattccgc gttgacaagt tcgctatcgt gcacaatgcg atcggcaaga agagcttctc
      721 caacgagcag cttttcgaca atgcgaagac gcttattcag gctgtcctga aagcgaggcc
      781 cgcctcggtg aagggaacct acgtgaagag ccttaatctt tcctcttcca tgggccccgg
      841 aatcaagatc gacgttgcgt ccgcgcagaa agaggttgca ggcgagtaac gcgcttcctt
      901 tcctctgctc aaaacgaaat atgaacttga actgaagaca gcgggtccct taaggggaca
      961 aaggccaggc cgcccgccga ggttccggtg agtcgatcgg catctgctgc cgtacaggta
     1021 ctcaatcccg ggcccggcgc ccgggatttt ttactttcac cgaaggaggt gagtacatgc
     1081 cggcaacact taagtacgaa caggtagatg aactgaaggg gcgtcttgcc cgcacacagg
     1141 ctattttcgc atgcgaatac cgcggaatga ctgttgcgca gattacggcg cttcgcgccc
     1201 aggtgcgcgc tgcaggcgga gaaatgaagg ttgcgaagaa tacgcttatg cgtattgcca
     1261 tggagcagga ggggtttatt ccattccccg acgatatggc gttcggaccg aacgtcttcg
     1321 cgctggcgta cggggatcct gtcgcggtag cgaaagctct tcgtgatttc gcgaaggaca
     1381 gaaccaacaa gttttttgtc ctgaaggggt gcgttcttgg acaaaccctt cttgatgcga
     1441 cacagattgg cgctctcgcc gatctcccgt ccaaggaagt ccttatcggt caggtcgtcc
     1501 gtaccatcgc ggccccgatt gcgggtctgc ttacggttct ttccggtcct gcaagaggat
     1561 tggtcacgtg cctcaacca
//
LOCUS       DAOSWW010000721         1577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69507_length_1577_cov_3.528909, whole genome shotgun sequence.
ACCESSION   DAOSWW010000721 DAOSWW010000000
VERSION     DAOSWW010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69507_length_1577_cov_3.528909"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PK442_12355"
     CDS             complement(<1..397)
                     /locus_tag="PK442_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOO88488.1"
                     /translation="MKELEEDIHGGAAMRLKVFLAVFLLLFVLSGLSNAEIQSGIRLD
                     RETARVEVGKSVVVRALPTGSGDVWWNSSDTSVADFVTASDGLSVTVRGWRTGTAVIS
                     VHFSPYGSEVIHSASCRVTVAPNSNPYRVA"
     gene            complement(535..886)
                     /gene="ssrA"
                     /locus_tag="PK442_12360"
     tmRNA           complement(535..886)
                     /gene="ssrA"
                     /locus_tag="PK442_12360"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(907..1368)
                     /gene="smpB"
                     /locus_tag="PK442_12365"
     CDS             complement(907..1368)
                     /gene="smpB"
                     /locus_tag="PK442_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOO88489.1"
                     /translation="MADRTVAQNRKARHDYFVLETFECGIVLTGTEIKSVRDGKINLK
                     EGYALIRGGELWLVGVHISPYEKGSYYNHEPLRDRKLLMKHHEILRLFSKVREKGLTL
                     VPLSVYLKEGKRAKVELALVKGKLLHDKRDSLAERDAKRDIERAVRRSDRD"
     gene            complement(1499..1576)
                     /locus_tag="PK442_12370"
     tRNA            complement(1499..1576)
                     /locus_tag="PK442_12370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1539..1541),aa:Pro,seq:cgg)
BASE COUNT          379 a          451 c          352 g          395 t
ORIGIN      
        1 cagccactct gtaaggattg gaattcggcg ccacagtcac ccggcatgaa gcgctatgaa
       61 ttacctccga gccgtaggga gaaaagtgta cggaaatcac cgccgtcccc gttctccacc
      121 cccttacagt taccgacaat ccatcggaag cggtaacgaa gtcagctacg gaagtatcgc
      181 tggaattcca ccatacatcg ccggatccgg tcggcaaggc acggacaact acggatttcc
      241 cgacttctac tcgcgctgtt tctctgtcaa ggcgaatgcc gctctgtatt tccgcattcg
      301 acaaaccgga aagaacgaac aacagcaaaa agaccgccag gaaaactttc aaccgcatcg
      361 cagcacctcc gtgaatatct tcttcgagtt ccttcatgta atagtatcac gcttttccgc
      421 ctcttgttgc gagaaaaatc tcgtgctttt gcagacggtg tagaatggtt tgataaaaaa
      481 accgccgctg ccaggatttt tactgagcaa cgacggtgaa atacgatttc ctattggtgg
      541 aggcgggggg aatcgaaccc ccgtccgaca agggccggcc gtgaaagcgc tacgggcgta
      601 gttcccgatt taatgtcggc ttgaaactcc cgggaacagg atttcctcgc ccaagccccc
      661 ttgtcttttt ttcgaagccg gagacactcc ccgaaaagca tccgcctttt tgtgtcgtct
      721 tacgaaaaaa cggcggctac ttcttcgtaa gacgtgactg cgctaagcag ccagtgcgta
      781 attattattg ttgacgtttg tttttgtgtc cgcgtttaac gaggttcgga cgacctcggc
      841 ccgctcttcc agaccctccg tcttgccgtc gaaaccagtc gcccccattt gtaaacgact
      901 gtaacttcag tccctatcgc ttcgccgcac ggcacgctct atatctcttt tcgcgtcccg
      961 ttcagcaagc gaatcccgtt tgtcgtgaag aagcttccct ttcaccaggg caagctcgac
     1021 tttcgccctc ttaccctctt tcaagtatac cgaaagagga accagagtca atcctttttc
     1081 gcgcaccttc gaaaaaagcc gaagtatctc atggtgcttc atcagaagtt ttctgtctcg
     1141 cagcggctcg tgattgtaat aacttccttt ttcatacggg gaaatatgaa cgcctacaag
     1201 ccataactct cctccgcgaa taagggcata cccctctttc aggttgatct ttccgtcgcg
     1261 cacagactta atctccgttc ccgtgagtac aataccgcac tcaaacgttt caagaacgaa
     1321 atagtcgtga cgtgcttttc tgttctgcgc tactgtgcgg tcggccatct tattttcctc
     1381 gttctacatt ctagcattaa ggtcagtatt agtcaataca aaaagcaaaa tacagggctc
     1441 ttcttataca aataaagagg ctccggaatt ccggaacctc tcgggagaat ccaaaatatg
     1501 gtcggggcga gaggatttga acctccgacc tcctggtccc gaaccaggcg cgcttaccag
     1561 actgcgctac gccccga
//
LOCUS       DAOSWW010000722         1577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69529_length_1577_cov_2.883049, whole genome shotgun sequence.
ACCESSION   DAOSWW010000722 DAOSWW010000000
VERSION     DAOSWW010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69529_length_1577_cov_2.883049"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /gene="flgA"
                     /locus_tag="PK442_12375"
     CDS             <1..327
                     /gene="flgA"
                     /locus_tag="PK442_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOO88490.1"
                     /translation="SAADVTVREVRVMRSQPLASSPEEVIGRSLGRNLTAGEPVLLNS
                     LSRTPIIQRGDSVIITVRKPGFLVEVRGEALDAGAEGESIRVRNLQSRAVIQAVVVGS
                     GRVEVQ"
     gene            331..909
                     /locus_tag="PK442_12380"
     CDS             331..909
                     /locus_tag="PK442_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOO88491.1"
                     /translation="MKRSACVLILLLCASVLTGCAAEAASLWNDNTNWIADRRPSQVG
                     DIVTVFVDEKTKTKDEGTTEASKTNDNNVDDGFGIFDFIRAFGFSSSSKMTGDGSTER
                     THSAKGQITCLVTDVLPNGNLVIEGTRDIATHEETLQLHVIGVIRPQDVDSYNKIRSD
                     LVANAEIDIKGKGTISRLQKPGILTQILQAIF"
     gene            923..>1577
                     /locus_tag="PK442_12385"
     CDS             923..>1577
                     /locus_tag="PK442_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOO88492.1"
                     /translation="MKRYGVFPLALLLLFITACGAAASHPDVRIKDLADVEGVRSNQL
                     VCLGLVMGLQGTGDRSAMATQMLRNIMSQFGISVNEKAVRSRNVAVVTLTAELPAFAR
                     PGQTIDVTASAMGDAKSLQGGTLLQAPLRAADGNVYAVAQGPLMVGGFAVEGAAGAVT
                     KNIVTVGRIPGGAIVERNVQTTFSAGRQINLLLRNPDFTTSERIAAAINSAYGGIASP
                     "
BASE COUNT          336 a          485 c          485 g          271 t
ORIGIN      
        1 tcagcggccg acgttacggt gcgcgaagtg cgcgtcatgc gttcacagcc gctggcctcg
       61 tcaccggagg aagtgatcgg gcgcagcctc ggaagaaacc ttacggcggg cgaacctgta
      121 cttctcaact ccctctctcg tactccgata attcaaaggg gagactctgt tataataacc
      181 gtaagaaagc cggggtttct cgtcgaggtc agaggagaag cccttgatgc gggcgccgaa
      241 ggagagagta tccgggtgcg taacctgcaa agcagagccg tgatccaggc agtcgtcgtt
      301 ggatccggaa gagtggaggt gcagtaagca atgaaacgaa gcgcgtgcgt tctcatcctt
      361 ctgctgtgtg cgtcggtcct cacgggctgc gcggcggagg ctgcgtcgct gtggaacgac
      421 aatacgaact ggatagccga ccgccgaccg tcgcaggtgg gcgatatagt gactgtcttc
      481 gtcgacgaaa agaccaagac caaggacgaa gggacgaccg aggcgagcaa gacgaacgac
      541 aacaacgtcg acgacggttt cggcatcttc gacttcatcc gcgcgttcgg cttcagctcg
      601 tcgagcaaaa tgaccggaga cggttccacc gagcggaccc acagcgccaa ggggcagatc
      661 acctgccttg tcaccgacgt cctccccaac ggtaacctgg ttatcgaggg aacgagagac
      721 atcgccaccc acgaggagac gcttcagctc catgtcatcg gcgttatccg gccccaggac
      781 gtggacagct acaacaagat caggagcgac cttgtcgcga acgccgagat cgacatcaag
      841 gggaagggta ccatctcccg tctgcaaaag cccggcatcc tgacgcagat ccttcaggcg
      901 atcttctaag gaggcgcgga ccatgaaacg gtacggagtt tttcctcttg cacttctttt
      961 gctcttcatc accgcctgcg gcgccgccgc ctctcacccc gacgtccgca tcaaggacct
     1021 cgccgatgtc gagggggtcc gttcgaatca gctcgtctgc ctcggccttg tcatgggcct
     1081 ccaggggacg ggcgaccgca gcgcgatggc cacgcagatg ctccgcaaca tcatgagcca
     1141 gttcggtatc tcggtgaacg aaaaggccgt gcgaagcaga aacgtggcgg tcgtcaccct
     1201 gaccgccgag cttcctgcgt tcgctcgtcc gggacagacg atcgacgtaa cagccagcgc
     1261 gatgggggat gcgaagagcc ttcagggggg cacgctcctt caggcgccgc tcagggcggc
     1321 ggacggcaac gtctacgcag tggcccaggg gccattgatg gtgggcgggt tcgccgtcga
     1381 gggagcggcg ggagcagtca ccaagaacat cgtcaccgtc ggcagaattc caggaggcgc
     1441 catcgtcgaa cggaacgtcc agaccacatt ctccgccgga cggcagatca atctgctgct
     1501 ccgcaacccc gactttacga cctccgagcg cattgccgcg gcgatcaact ccgcctacgg
     1561 aggcatcgcc tcccctg
//
LOCUS       DAOSWW010000723         1576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69616_length_1576_cov_2.538462, whole genome shotgun sequence.
ACCESSION   DAOSWW010000723 DAOSWW010000000
VERSION     DAOSWW010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69616_length_1576_cov_2.538462"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PK442_12390"
     CDS             <1..226
                     /locus_tag="PK442_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88493.1"
                     /translation="DEETPVCTELERIGRHPVKDHVILIDDARCFGADSGYPDIPQVE
                     EMARRLFPDSTFAVADDIIRIVPRTGNDGR"
     gene            223..1206
                     /locus_tag="PK442_12395"
     CDS             223..1206
                     /locus_tag="PK442_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HOO88494.1"
                     /translation="MIVKLTTPSASWPLALQTPGASGKWGDVQFVIDRDCAHADAWVV
                     YEKLPGRMTTVCPPSRTILITGEPPMIKSYPEAFAAQFAKVLTCHAMRHGGVISEQQA
                     LPWHYGREFDQSGNERFTESYDSLVRNDPFAAKSRLLSIICSTKRKRDGHRKRHEFVS
                     SLEQAPLPGLDIFGKGRAKEAACKRDAIAPYKYHIVLENSECADYWTEKLADAYLGGA
                     FPFYWGCPNIEKYFPEGAFARIDIERPEEAVRTIMATIEQGTYERSIPLLREARDRVL
                     DGYNLFPAIVSLLRTLPDEPPAAVTLRPEESFTLRGRIRRLRMLFRAQSSR"
BASE COUNT          349 a          479 c          443 g          305 t
ORIGIN      
        1 agatgaggag actcccgtct gcacggagct cgaacgcatc ggacggcatc ctgtgaaaga
       61 ccacgtgatt ctgatcgacg acgccagatg cttcggcgcg gactcgggat acccggacat
      121 cccgcaggtc gaggagatgg cccggcggct ctttccggat tccaccttcg ccgtcgcgga
      181 cgacatcatc cgcatcgttc cccgaaccgg caacgacggc agatgatcgt caaactcacc
      241 accccctcgg cgtcgtggcc gcttgccctg cagacgccgg gtgcatcggg aaagtggggg
      301 gacgtacagt tcgtgatcga ccgggactgc gcccacgccg acgcgtgggt tgtctacgaa
      361 aaactccccg ggaggatgac gacggtttgc ccgccgtcgc gaacgattct gatcaccggc
      421 gagccgccga tgatcaagag ctacccggag gcgttcgcgg cgcagttcgc caaggtgctc
      481 acctgccacg cgatgcggca tggaggagtt atttccgaac agcaggcgct tccgtggcac
      541 tacgggcgcg agttcgacca atcggggaac gagcggttca ccgaaagcta cgattccctc
      601 gttcgaaacg accccttcgc agcgaagagc cgccttctct ccatcatctg ctcgacgaag
      661 cggaagcgcg acgggcacag gaaacggcac gagttcgtct cctccctgga gcaagcgcct
      721 cttccggggc tcgacatctt cggcaaagga agggccaagg aagcggcgtg caaacgcgac
      781 gcgatcgcgc cctacaagta ccacatcgtc ctcgaaaact cggagtgcgc cgactactgg
      841 acggaaaagc tggcggacgc ctacctcggc ggggcgtttc cattctactg ggggtgtccc
      901 aacatcgaga agtactttcc ggaaggggct ttcgcccgca tcgacatcga acgtcccgag
      961 gaggccgtcc ggacgatcat ggcgactatc gagcagggaa cctacgaacg gtcgattccg
     1021 cttttaaggg aggcgcgcga ccgtgtgctc gacgggtaca acctgtttcc cgcgatcgtc
     1081 tccctcctcc ggacgcttcc ggacgaacct cccgcggcgg tgacgctccg tccggaggag
     1141 agtttcacgc tgcgaggaag gatcaggcgt ctcagaatgc tgtttcgggc gcagtcatcg
     1201 agataggcag catcgttctc caagaaaaaa tctctggaat cgcatccaca ccaacctgtg
     1261 gtgaacttta ggattgggtc tattccttta tccttaaacg tgttattttt ttctcctatt
     1321 tttttcgcag ccatcaaaaa acccctgatt gttgcgaagg ttccacactc acgtgacaac
     1381 ttcaggaacg agcgagctaa agcccaagcg aatgagaacc gccccatcaa gtaatgatca
     1441 gccgatgtaa ttttcattga aagataccta ttgtataaat acatcgccgc cttcttctcc
     1501 gctcctacaa ttcttgagcc agacacagaa tggtgcgaca cagacccatt tgatgccaca
     1561 agaagaggag taccgt
//
LOCUS       DAOSWW010000724         1575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69665_length_1575_cov_4.216447, whole genome shotgun sequence.
ACCESSION   DAOSWW010000724 DAOSWW010000000
VERSION     DAOSWW010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69665_length_1575_cov_4.216447"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK442_12400"
     CDS             <1..598
                     /locus_tag="PK442_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOO88495.1"
                     /translation="FIDKTDHFFLNIAMAAGKASLEAAAGIEGSSMLTVMARNGTEFG
                     LQVSGLGDRWFTCEAALPDVLLFPGFTREDTNRDIGDSAIMETYGVGGFALAAAPAIV
                     QFIGGTPKDAANYTLEMYEITAGENSMFTIPALNFRGTPTGIDVLKVVETGITPVLDT
                     GAAHKEPGKGQVGAGIVRMPMDAFVKAAEAFADKYLSE"
     gene            639..1442
                     /locus_tag="PK442_12405"
     CDS             639..1442
                     /locus_tag="PK442_12405"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOO88496.1"
                     /translation="MSNSLELKNWSNIKVYDLTIPIGIQTPPWPTYEPLQVKYFKRLA
                     PNGANGQLLTHSNHVGTHLDGPIHFCGHGGDIASLPLKDFLVGPGVVVDIQDIAEDYG
                     IYTPEDLEARADIREGDILIINTGYHRYGWNEPEADEVRYMVKHPGPTREFATWALKK
                     KIKWIGVDCGSADHPMNTKIREWMPVQARQADAHLKKKYGKGLDDFFPEEDYQVMHIA
                     LFPSNLIHAECVAGDIDKLSGKRVTIGCFPWRFVGGESCISRIVAFAEE"
BASE COUNT          338 a          506 c          456 g          275 t
ORIGIN      
        1 cttcatcgac aagaccgacc acttcttcct gaacatcgcg atggcagcgg gaaaggcctc
       61 tctcgaagcg gccgcaggga tcgaaggttc gagcatgctc accgtcatgg cgcgcaacgg
      121 cacggagttc ggcctccagg tctccgggct cggcgaccgc tggttcacct gcgaagcggc
      181 cctgccggat gtgcttctct tccccgggtt cacccgcgag gacacaaacc gcgacatcgg
      241 cgacagcgcc atcatggaga cctacggcgt aggcggcttc gcgctcgccg cggccccggc
      301 catcgtccag ttcatcggcg gcacgccgaa ggacgcggcg aactacacgc ttgaaatgta
      361 cgagattacc gcgggagaga acagcatgtt caccatcccc gcgttgaatt tccgcggcac
      421 gccgacgggg atcgacgtcc tcaaggttgt ggagacggga atcaccccgg tgctcgacac
      481 aggggcggcg cacaaggagc ccggcaaggg gcaggtcggc gcgggtatcg tccgcatgcc
      541 gatggacgcc ttcgtgaagg ccgccgaagc cttcgcggac aagtatttga gcgagtaatt
      601 cttatccaag tttaataaaa tacaaggagg tacttactat gtccaacagt ctcgaactca
      661 agaactggag caatatcaag gtgtacgatc ttaccattcc catcggcatc cagacgccgc
      721 catggcccac ctacgagccg cttcaggtga agtacttcaa gcgcctcgct cccaacggag
      781 cgaacggcca gctgctcacc cactccaacc acgtgggaac gcacctcgac ggcccgatcc
      841 acttctgcgg ccacggcgga gatatcgcca gccttccgct gaaggacttc cttgtcggcc
      901 ccggcgttgt ggtcgacatc caggatatcg ccgaggacta cggcatctac actcccgaag
      961 accttgaagc ccgcgccgac atccgcgagg gcgatatcct catcatcaac accgggtacc
     1021 accggtacgg ctggaacgag cccgaggccg acgaagtccg ctacatggtg aagcaccccg
     1081 gacccaccag agagttcgcc acctgggcgc tcaagaagaa gatcaagtgg atcggcgtcg
     1141 actgcggttc cgccgaccat ccgatgaaca cgaagatccg cgagtggatg ccggttcagg
     1201 ccagacaggc cgacgcgcac ctgaagaaga agtacggcaa ggggctcgac gatttcttcc
     1261 ccgaagagga ctaccaggtg atgcacatcg ccctcttccc cagcaatctg atccacgccg
     1321 agtgcgttgc gggcgacatc gacaagctca gcggcaaacg ggtcaccatc ggttgcttcc
     1381 cgtggagatt cgtgggcggc gagtcctgca tctcgcgcat cgtcgcgttc gccgaagaat
     1441 aactccgtta cgacgggcgt cctcctcctc atggttttca gagggttcgg ggacgccctc
     1501 ttcacatact gattcctcac cgaaggggtg atatgtcaat gctggctttt gaaatggaac
     1561 gccccagatc gctgg
//
LOCUS       DAOSWW010000725         1574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69747_length_1574_cov_3.607637, whole genome shotgun sequence.
ACCESSION   DAOSWW010000725 DAOSWW010000000
VERSION     DAOSWW010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69747_length_1574_cov_3.607637"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1574
                     /locus_tag="PK442_12410"
     CDS             <1..>1574
                     /locus_tag="PK442_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88497.1"
                     /translation="ANGGRSWRHIGSGISSGNFYSLRVSPKGHIVLSTFEEVLVSKNG
                     GVSWSSLRGGGYVKDFLFTRSETFLSVHWRGGFNYASTENQGEWERSRGNVDFLITGV
                     LEAENKRLWGATFGGGVRVSDDGGKNWSAFGDGPENRSVLSLAWNGSTGTLFAGTYEG
                     GVFARQGEEAWRSASEGLPPASIVQALAVAKDGSVWAGTHRDGCFVSRYGGRGWESFP
                     EGGGNGVSVTAIEPFKEGVVIGTASGGLFYAEAALSSWSPLLPFEPVTGLVELDKGHV
                     LAVARSGRLFRSSDGGRTWRNDGEIPTGEGGCAVLLRTADGALLAGGAGGVYRSDGST
                     WTRSGFPAKATDPSVISPQHGAPELDPVIAMAESGGNVFAATWRNGLLRSRDGGRSWE
                     HTEGNDGVYEITPSHSVTAEYRYVYALSSDGGRVAIATDRGFSASVDGGETWKNSYVN
                     SGLFSVLFDREGTLWGVSRNGVWRCTPEDMNVEKLDIEGFDWSPFSYFTDLFRGSGGT
                     VFGMTDNELHRLLPKD"
BASE COUNT          264 a          471 c          570 g          269 t
ORIGIN      
        1 ccgcgaacgg gggaagaagc tggcgtcata tcggaagcgg catctcttcc ggcaactttt
       61 actcgctgcg cgtttccccg aagggacata tcgtcctttc gaccttcgag gaggtgctgg
      121 tctcgaagaa cggaggcgtg tcgtggtcgt ccctgagggg aggcggctac gtgaaggact
      181 tcctctttac gcgatcggag accttcctga gcgtccactg gcgcggggga ttcaactacg
      241 cgtcgacgga aaatcaagga gagtgggagc gcagcagggg gaacgtcgat tttctgatta
      301 ccggcgtcct tgaggcggag aacaaacgcc tgtggggcgc gaccttcggc ggcggagtcc
      361 gcgtctcgga cgacggcgga aaaaactgga gcgcattcgg cgacggaccg gagaaccgct
      421 cggtcctctc gctggcgtgg aacggctcga ccgggacgct cttcgccgga acctatgaag
      481 gcggcgtctt tgcgcgccag ggagaggaag cgtggcggag cgcgtcggag gggctgcctc
      541 cggcctcgat cgtccaggcg ctcgcggtgg cgaaggacgg atcggtgtgg gcgggaaccc
      601 accgtgacgg ctgcttcgtc tcgcggtacg gggggcgagg ctgggagtcc tttcccgagg
      661 gcggcggcaa cggcgtttcc gtgacggcga tcgagccttt caaggagggc gtggtgatcg
      721 gaaccgcatc cggagggctt ttctacgcgg aagcggcgct ctcttcgtgg agcccgctcc
      781 tcccgttcga gccggtcacg gggctcgtgg aactcgacaa ggggcacgtc ctcgcggtcg
      841 cgcgctcggg aaggctgttc cgctcctccg acggagggcg cacgtggaga aacgacggtg
      901 agattcccac tggcgaaggg ggctgtgcgg tcctgctgcg gacagccgac ggcgcgctgc
      961 tcgccggggg cgcgggcggc gtgtaccgtt ccgatggaag cacgtggact cgctccggct
     1021 tccccgcaaa ggcgaccgac ccgagcgtca tctcacctca gcacggcgcg ccggagctcg
     1081 atccggtgat tgcaatggcc gagagcggag gcaacgtgtt cgcggcgacg tggcggaacg
     1141 gccttctccg ctcgcgcgac ggcggacgga gctgggagca taccgagggg aacgacggcg
     1201 tctatgaaat cactccttcg cactccgtca cggcggagta caggtacgtc tacgcgctga
     1261 gttcggacgg cggccgcgtc gccatcgcca ccgaccgggg gttctccgcc tccgtcgacg
     1321 gcggggagac ctggaagaac tcctacgtga actccgggct cttctccgtc ctcttcgacc
     1381 gggaagggac gctctgggga gtgtcccgaa acggcgtctg gcgctgcacc ccggaggata
     1441 tgaacgtcga aaaacttgat atcgaggggt tcgattggtc gccgttcagc tatttcaccg
     1501 acctcttccg cggaagcggc gggacggtgt tcggcatgac ggacaacgaa ctgcaccggc
     1561 tgcttccgaa ggac
//
LOCUS       DAOSWW010000726         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69840_length_1573_cov_2.664032, whole genome shotgun sequence.
ACCESSION   DAOSWW010000726 DAOSWW010000000
VERSION     DAOSWW010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69840_length_1573_cov_2.664032"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PK442_12415"
     CDS             complement(<1..339)
                     /locus_tag="PK442_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter permease subunit"
                     /protein_id="HOO88498.1"
                     /translation="MFPESFQVHVAPAVNRFINDLIAAYAPVFDSISAGILSFLLTVS
                     EILVFIPWWAYILAVGLLSWYSSKKFVASIVLATLPFLIGMFGLWAMALESLSIIITA
                     VLMALLIGLPV"
     gene            complement(329..1225)
                     /locus_tag="PK442_12420"
     CDS             complement(329..1225)
                     /locus_tag="PK442_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163687.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HOO88499.1"
                     /translation="METPSEAIVIRDLWKIYSRKKDLRISSSDVENEERVEELDKGED
                     AVVAMKNVSLSVNRGETFIIMGLSGSGKSTLIRCLIRLVEPTSGEIIVNGENVTMMHK
                     RELVDFRREKIAMVFQHYGLLPHRTVLDNVCFGLKLRGEEKEARRAKGVEALEQVGLK
                     GWEKYYPASLSGGMRQRVGIARALVMDASILLMDEPFSGLDPLIRRELQDEMIRLQKE
                     MHKTIFFVTHDLSEALRMGDRMAIMKKGEIVQVGSPDEVIASPADEYVAKFVQDERDQ
                     VQRAEQVLAREHTRKEAEAHVS"
     gene            complement(1227..>1573)
                     /locus_tag="PK442_12425"
     CDS             complement(1227..>1573)
                     /locus_tag="PK442_12425"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOO88500.1"
                     /translation="WVMGLLDMTRLEEPPYDEALWTEEKNFACGFPSVRVLIVANSKF
                     VKENPEIADFLRNYSTTLEQNNKALAFMFQNDADTQQAAVWFLREYDDIWSKWLPAEA
                     AEKVRAALKEEK"
BASE COUNT          334 a          449 c          436 g          354 t
ORIGIN      
        1 caccggcaac ccgattaaaa gggccatgag aacagcggtg atgatgatgg agagactctc
       61 aagcgccatg gcccacagac cgaacatccc gatgagaaag gggagcgtcg ccaaaacgat
      121 ggaggcgacg aatttctttg acgaatacca tgaaagcaag cctaccgcaa ggatgtacgc
      181 ccaccaggga ataaaaacga gtatctcgct gacggtgaga agaaaggaga gaattccagc
      241 ggagattgag tcaaaaacgg gtgcgtaggc cgctatcagg tcgttgatga acctgttgac
      301 ggccggcgcc acgtgtactt gaaaagactc aggaaacatg ggcctccgcc tcctttctcg
      361 tatgctctct cgccagaacc tgttcggccc gctgcacttg gtcgcgttcg tcctgaacga
      421 acttcgccac gtactcgtcg gcagggcttg cgatgacttc gtcgggagag cctacctgta
      481 ctatctcccc ttttttcata atggccatcc ggtctcccat tcgaagtgcc tcggagaggt
      541 cgtgcgtcac gaagaagatg gtcttgtgca tctccttctg cagacggatc atctcgtcct
      601 gcagttcgcg ccggataaga gggtccagtc cgctgaacgg ctcgtccatg agaagtatcg
      661 aagcgtccat cacgagcgcg cgggcgatac cgaccctctg tcgcataccg ccgctcaggg
      721 atgcgggata gtacttctcc caccccttga ggccgacctg ttcaagggcc tctacgccct
      781 ttgcgcgccg ggcttccttc tcctctcccc ggagtttgag tccgaagcag acgttgtcga
      841 gcacggtgcg gtgaggcaga agtccgtagt gctggaagac catggcgatc ttttcgcggc
      901 ggaaatccac cagttctctc ttgtgcatca tcgtgacgtt ttcgccattc acgatgattt
      961 caccggaagt cggctcgaca aggcggataa ggcagcggat aagggtagac ttgccgctgc
     1021 ccgagagccc catgatgatg aacgtctccc cgcggttcac ggagagggag acgttcttca
     1081 tcgcgacgac agcgtcttcg cccttgtcga gttcttcgac gcgttcttcg ttctcgacgt
     1141 cggaagacga tattcgcagg tcctttttcc gggagtagat tttccacaaa tcccggatga
     1201 ctatcgcctc ggaaggcgtc tccaaattat ttctcctcct tcaacgcggc gcgaaccttc
     1261 tcggcggctt ccgcggggag ccatttgctc caaatatcgt catattcgcg gaggaaccag
     1321 acggccgcct gctgcgtatc cgcgtcgttc tggaacatga acgcgagcgc tttgttgttc
     1381 tgttcaagcg tcgttgaata gttgcgaagg aaatcggcga tttccgggtt ttccttcaca
     1441 aatttgctgt tggcgacgat aaggactctg actgaaggga agccgcaggc gaaatttttc
     1501 tcctccgtcc agagcgcttc atcgtacgga ggctcctcca ggcgtgtcat atccagcagg
     1561 cccatgaccc aag
//
LOCUS       DAOSWW010000727         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69864_length_1573_cov_2.119236, whole genome shotgun sequence.
ACCESSION   DAOSWW010000727 DAOSWW010000000
VERSION     DAOSWW010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69864_length_1573_cov_2.119236"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PK442_12430"
     CDS             <1..718
                     /locus_tag="PK442_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOO88501.1"
                     /translation="GFPAYLPTRIAEFYERAGKVTTLGGTRGSISIIGAVSPPGGDFT
                     EPVTRHTKRFIRCFWALDQQLARSRHFPAISWIDSYSEYGDEVKSWYASHVDPAWATL
                     REKARTLLAEDDKIQQIIRLVGEDVLPDDQRLIALTASFLKNGYLQQGAFGPDSYCPP
                     KKGIAILNLLIAFHQKAEDLLSQGCPLSLLKGIDELVELTHLREIPEDRLERFEELKH
                     TLFERMESINRERIVSRREV"
     gene            722..>1573
                     /locus_tag="PK442_12435"
     CDS             722..>1573
                     /locus_tag="PK442_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOO88502.1"
                     /translation="MSATEYIGVRKITGPFVLLDGIQGAGYDEIVLVSTPDGEARKGR
                     VVLVDEKATLVQVFTGTEGLVPETASVRFLGRELEMALAPSMLGRTFSGLGEPRDGYG
                     PVLGGVRRPVRGAPINPMAREYPRNFISTGISSLDVLLTLIRGQKLPIFSGNGLPHNA
                     LAVQIATQSKLLGAENFVVVFAGIGIKNDDAAYFQSHLASEGRGGNLVLFLNLADDPV
                     IERIATPRLALTTAEYLAFDLGMHVLVILTDMTSYCEALRELSVARGEVPSRKGYPAY
                     LYSDLASL"
BASE COUNT          263 a          495 c          551 g          264 t
ORIGIN      
        1 gggcttcccg gcctaccttc ccacgcggat cgcggagttc tacgagcgcg ccggcaaggt
       61 gaccacgctg ggcggcacgc gggggagcat ctcgatcatc ggcgcggtct cccctccggg
      121 gggcgacttc accgagccgg taacgcggca cacgaagcgc ttcatccgct gcttctgggc
      181 gctggaccag caacttgccc gatcgcgcca cttccccgcc atcagctgga tcgactcgta
      241 cagcgagtac ggcgacgagg tgaagtcatg gtacgcctcg cacgtggacc cggcgtgggc
      301 gacgctccgc gagaaggcgc ggacgcttct tgccgaggac gacaagattc agcagatcat
      361 ccgcctggtg ggcgaggacg tgcttccgga cgaccagcgg ctgatcgcgc tgacggcgtc
      421 gtttttgaag aacggctacc tgcagcaggg ggctttcggc cccgactcgt actgcccgcc
      481 gaagaagggg atcgcgatct tgaatctgct gatcgccttc caccagaagg cggaggacct
      541 gctttcgcag gggtgcccgc tctcgctgct gaaggggatc gacgaactgg tggagctgac
      601 gcatctgcgg gagattccgg aggatcggct cgaacgcttc gaggagctga aacacaccct
      661 cttcgaacgc atggagagca tcaaccggga gcgcatcgtc tcccggaggg aggtgtagac
      721 cgtgtcggca acggaatata tcggcgtgcg gaagatcacc ggccccttcg tgcttctgga
      781 cggcatccag ggcgcggggt acgacgagat cgtgctcgtc tccactcccg acggagaggc
      841 gcgcaagggg cgcgtggtgc tggtcgacga aaaggcgacg ctggtgcagg tcttcaccgg
      901 gacggagggg ctcgtccccg agacggcctc agtacgcttt ctcgggcgcg agcttgagat
      961 ggcgctggcg ccgtcgatgc tcgggcggac gttctccgga ctgggagagc cccgcgacgg
     1021 gtacggcccg gtcctgggcg gggtcaggcg tccggtgcgc ggcgcgccga tcaacccgat
     1081 ggcgcgcgag tacccgagga acttcatctc gacggggatc tcgtcgctcg acgtgctgct
     1141 gacgctgatc cgcggacaga agctgccgat cttctcgggc aacggactgc cgcacaacgc
     1201 cctcgcggtg cagatcgcga cgcagtcgaa gctgctgggg gcggagaatt tcgtggtcgt
     1261 cttcgccggg atcggcatca aaaacgacga cgcggcgtac tttcagtcgc acctcgcctc
     1321 ggaggggcgg gggggcaatc tggtgctctt cctgaacctg gcggacgacc ccgtgatcga
     1381 gcgcatcgcg acgccgcgcc tcgcgctgac gaccgcggag tatcttgcct tcgatctggg
     1441 gatgcacgtg ctcgtgatcc tgacggacat gacgagctac tgcgaagcgc tcagggagct
     1501 gtcggtggcg cgcggggagg tgcccagccg caaggggtac cccgcgtatc tgtacagcga
     1561 tctcgcctcc ctc
//
LOCUS       DAOSWW010000728         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_69874_length_1573_cov_1.611989, whole genome shotgun sequence.
ACCESSION   DAOSWW010000728 DAOSWW010000000
VERSION     DAOSWW010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69874_length_1573_cov_1.611989"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..>1573)
                     /locus_tag="PK442_12440"
     CDS             complement(89..>1573)
                     /locus_tag="PK442_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88503.1"
                     /translation="FCVSSPVIPNAAASQKARGFEEIALAIEALIPEAEKSILAGVAS
                     VELLLKSSKTEASEMTGAWNTVQEQFFNSSPVGELRVQLTAFRMTMLDEKLNESDSKT
                     SSSGGSGAPERSAKGARITREIANDIYNEGRELADFAQEILSVGDAVAWTLKVNRHIQ
                     SLKFDIENAPVRVGVYVKEMREVSALLVEIIAKVKRGVELRKRVNVSAADLAVLRNEV
                     AQSLKSVVLMRAGTVNAALSLANTTKYLEPGEGYVVPEAEAKRIAVLAEYWKDAKNLY
                     PLVRSDILDAVAKWAFFPKLKWEVYGETRQRFVDVYGPLLAGNIFKGIPLFEGKKYIE
                     LPAVVINVENTLRALLENIDDLHKDMEQRRKALELEEVLTQSERMRLTVLSNMYSPVM
                     ARNLVVAASRAGGGANRVLELERLKRGVAKDSEEYTKYDVEQKLLQSRKHPEQLKADA
                     AVSLFVKGLEDARKTAKDIVEKHTARRKALGLPPALKEITLAKT"
BASE COUNT          306 a          494 c          392 g          381 t
ORIGIN      
        1 gagaatcaga acgctcatca ccttcatgca cacgcccgat gtgcccttgt acatgcgtca
       61 ttcctcctct tcggcaaaaa acccaccgtc aagtcttcgc cagcgtgatc tccttgagcg
      121 ccggtggaag accaagcgct tttcgccgcg cggtatgctt ttccacaata tccttcgcgg
      181 tttttcgagc gtcttccaga cctttgacga agagactcac agcagcatcg gcctttaact
      241 gttccggatg cttccggctt tgcagcagct tctgttccac atcgtacttc gtgtactctt
      301 cgctgtcctt ggctacccct cgtttcaggc gttccagttc gagaacgcgg ttcgctccgc
      361 ctcctgccct ggacgccgcg acaacaaggt ttctggccat cacggggctg tacatgttcg
      421 aaagaaccgt gagccgcatg cgctcgctct gcgtcagcac ctcctcaagc tccagcgcct
      481 tccgccgctg ctccatatct ttgtgcaggt cgtcgatatt ctccagaagc gcgcgaagag
      541 tgttttccac attgatgacc acggcgggga gttcgatgta cttcttccct tcgaagagag
      601 ggatcccctt gaagatgttt cccgcgagaa gcggtccgta gacatcgacg aatcgttgcc
      661 ttgtctcgcc gtacacctcc catttcaact tggggaaaaa cgcccatttc gctaccgcat
      721 ccagtatgtc gctacgaaca agcggataga gattcttcgc gtccttccag tactccgcaa
      781 gaacggctat tctcttcgcc tcggcttcgg ggacgacgta cccctctccc ggctcaaggt
      841 acttggttgt attcgcgaga cttaacgccg cgttcaccgt cccggctctc atcaggacaa
      901 cgcttttgag cgactgggca acttcgttcc ggagaaccgc cagatccgcc gccgagacgt
      961 ttacgcgttt ccgaagctcc acgccgcgct tcaccttggc aatgatctct accaacaaag
     1021 cgctgacctc acgcatctcc ttcacgtaga cgccgacccg aacgggcgcg ttttctatat
     1081 cgaacttgag ggattgtatg tgccggttca ccttgagcgt ccacgccaca gcatcgccca
     1141 cggagaggat ctcctgggca aaatccgcaa gttcccttcc ctcgttgtag atatcgttcg
     1201 caatctcgcg ggttatccgc gctccctttg cagagcgttc aggagcgccg ctccccccgc
     1261 tggaggaggt cttcgaatcg gattcgttta atttctcatc gagcattgtc atacggaacg
     1321 ctgtaagctg gactcgcagc tctccgacag gggatgaatt gaagaactgc tcctgaacag
     1381 tgttccatgc tcccgtcatt tcgctcgcct ctgtttttga agacttgagc aggagctcca
     1441 cagaagcgac tccggcgagg atactcttct cagcttcagg gatgagggct tcgatggcaa
     1501 gggctatctc ctcgaacccc cgcgctttct gggaagctgc ggcgttggga atcacagggg
     1561 aagagacgca gaa
//
LOCUS       DAOSWW010000729         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_70121_length_1569_cov_4.165125, whole genome shotgun sequence.
ACCESSION   DAOSWW010000729 DAOSWW010000000
VERSION     DAOSWW010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70121_length_1569_cov_4.165125"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1151)
                     /locus_tag="PK442_12445"
     CDS             complement(48..1151)
                     /locus_tag="PK442_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO88504.1"
                     /translation="MANKMITAVINGKTITAESGSTILQAARANNIYIPTLCDHPSLP
                     PSGACRVCLVEAERNPKLLTACTTPLTEGMVVSTNSPRVLSARKAVVEMLLIRHPLDC
                     FSCESNGRCELQNLAYELGIEKSPFTDEGDVCTHFELDNTNPFFIRDMNKCILCGRCV
                     RACDWRAGYHAIDFQFRGIHTQINPPIGTKLEDEASDCVFCGQCVQVCPVGALVEKKS
                     VGQGRPWQTHVVRTTCPYCGVGCQLDLHMNGEQIARVTGTEDGMPNLGRLCVKGRFGY
                     DFIYSDERLTKPLIRERKGEKKGPDDTFREASWDEALDLVASRLKEVMRKHGPDAVGG
                     VSCARSINEDSYSMQKLYRMVFNTNNIDHCARV"
     gene            complement(1179..>1569)
                     /locus_tag="PK442_12450"
     CDS             complement(1179..>1569)
                     /locus_tag="PK442_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOO88505.1"
                     /translation="VPDDRSAIEGAISGWLDEGAELVLCTGGMSVDADDMTPSAIRAV
                     CDSVLFRRTPVIPGANLMLAAKDGAFVLGVPASAVFIERTSLDILLDRLFAGVPPDGK
                     EVRGWGRGGLCAQCDSCGYPRCAFAAR"
BASE COUNT          268 a          486 c          496 g          319 t
ORIGIN      
        1 ccgcccgctc caaagcttgt cagtagcccc gctactgtag gagcgtgtca gacgcgtgca
       61 cagtggtcta tgttgttcgt gttgaagacc atgcggtaga gtttctgcat gctgtaggaa
      121 tcttcgttga tactgcgcgc gcagctcact ccacccaccg cgtccgggcc gtgcttccgc
      181 atcacctcct tcagtcggga tgcgacgagg tcgagcgcct cgtcccagga tgcctcgcgg
      241 aacgtatcgt cgggcccctt cttctcgccc ttgcgctcgc ggatgagggg cttcgtaagc
      301 cgttcgtcgg agtagatgaa gtcgtagccg aagcgtccct tgacgcagag acgcccgagg
      361 ttcggcatgc cgtcctccgt gccggtgacc ctggcgatct gctcgccgtt catgtgcagg
      421 tcgagctggc agccgacgcc gcagtacggg cacgtcgtgc ggacgacatg cgtctgccag
      481 ggcctgccct gcccaaccga cttcttctcg acgagcgccc cgaccggaca gacctggacg
      541 cactgcccgc agaagacgca gtcggaggct tcgtcctcaa gcttcgtccc tatgggcggg
      601 ttgatctgcg tgtggatccc tctgaactgg aagtcgatgg cgtggtaacc ggctctccag
      661 tcgcaggcgc ggacgcagcg gccgcagagg atgcacttat tcatgtcgcg gatgaagaag
      721 gggttcgtat tgtccagttc aaaatgcgta cagacgtcgc cctcgtcggt gaagggggat
      781 ttttcgatgc cgagttcgta ggcgaggttc tgcagttcgc acctgccgtt ggactcgcag
      841 gagaagcagt cgagcggatg gcggatgagc agcatctcga cgacggcttt gcgcgccgaa
      901 aggaccctgg ggctgttcgt ggacacgacc atgccctcgg taagcggcgt cgtgcaggca
      961 gtgaggagtt tcgggtttct ctccgcctcg acaagacaga cgcggcacgc gccgctcgga
     1021 ggcagagacg gatgatcgca gagcgtcggg atataaatgt tgttggcccg ggccgcctgg
     1081 aggatcgtgg acccggactc cgccgtgatc gtttttccgt taatcacagc ggtgatcatt
     1141 ttgttcgcca tggttcagtc tccttttctt ttttctcgct atcgcgccgc gaaggcgcat
     1201 cgcggatagc cgcaggagtc gcactgagcg cacagtcccc cccttcccca gcctctgacc
     1261 tctttgccgt cgggcggaac gcccgcgaag agcctgtcaa ggaggatgtc gagcgacgtc
     1321 ctttctatga acacagccga tgccggaacg ccgaggacaa acgcgccgtc cttggcggca
     1381 agcatgagat tggccccggg aatcaccggc gttcttcgaa acagtacgct gtcgcacact
     1441 gcgcggatcg cggagggcgt catgtcgtcg gcgtccacgc tcattccgcc ggtacagagc
     1501 acaagctccg cgccttcgtc cagccagccg gagatcgcgc cctcgattgc tgaacggtcg
     1561 tccgggacg
//
LOCUS       DAOSWW010000730         1566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_70382_length_1566_cov_3.429517, whole genome shotgun sequence.
ACCESSION   DAOSWW010000730 DAOSWW010000000
VERSION     DAOSWW010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70382_length_1566_cov_3.429517"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1011
                     /locus_tag="PK442_12455"
     CDS             232..1011
                     /locus_tag="PK442_12455"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOO88506.1"
                     /translation="MEFLSSFTGLDLSLWSWVVVLSAGAGVGIAKTAVPGSGILVVAL
                     MAMTLPSRLSVGVMLPMLIFGDLFAVGKFWMHTDKRRLLTLLLFALPGLGVGYMVLRF
                     ASEDQLRPIIGGVVLAMLILHQVSRVLKQRRGAAAAADGKTSPLLTAVFGFFAGVGTG
                     MANASGPVMSLYLLIAGLPKLPFIAVTAWFFFIMNLLKVPLFFSLGLITMQSLRINML
                     ALPSIAAGALLGYWIVKRISQERFNQIVLTLAFVAALRLLT"
     gene            complement(1235..1555)
                     /locus_tag="PK442_12460"
     CDS             complement(1235..1555)
                     /locus_tag="PK442_12460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOO88507.1"
                     /translation="MVQYGVYWTDLDPTQGAEMNKTRPCVVVSPPEMNMHLLTVLVAP
                     VTSRQRESYPTRVHFSIGDVSGWIVLDQLRAIDRKRLRRKIGCLDGETALLVKNVIRE
                     MLVD"
BASE COUNT          288 a          485 c          426 g          367 t
ORIGIN      
        1 cgccccgaga aggacgctga gcgtttcact cttgcttcgc gcttgactct tttccagttc
       61 cgacataatg atacctcccg attataatga tggtgcattc cttcttgcac tgcaagcatt
      121 atacccgatt ttcccctgcg ttttgcaagg gatagcctgt agtttttctg aatacaaaag
      181 acatgatgta taatggcggc actgcgcagt cattgaaagg tggtatcctc aatggagttt
      241 ctctcttctt ttacggggct cgacctctcc ctgtggtcgt gggttgtcgt cctcagtgcg
      301 ggcgcgggcg tcggcatcgc aaagacagcc gttccgggca gcggcatcct cgtcgtcgcg
      361 ctgatggcga tgacgctccc ctcgcgtctc tccgtcggcg tgatgctccc gatgctgatt
      421 ttcggcgacc tcttcgccgt gggaaaattc tggatgcaca ccgacaaaag gcgtcttctg
      481 acgctgctgc tcttcgctct tccggggctc ggcgtggggt acatggtgct gcgctttgcg
      541 agcgaagacc agttgcggcc catcatcgga ggcgtggtgc tcgcaatgct catcctgcac
      601 caagtctcgc gcgtcctgaa acaacggcgc ggggcggctg cagcggccga cgggaaaaca
      661 tctccgctgc tcaccgcagt tttcggcttc ttcgccggag tcggaacggg aatggcgaac
      721 gcttccggcc cggtaatgag cctctacctg cttatcgcag gactgccgaa acttcccttc
      781 atcgccgtca ccgcgtggtt ctttttcatc atgaacctgc tgaaggttcc gctcttcttc
      841 agcctggggc tcatcaccat gcagagcctc aggatcaaca tgctcgccct cccctccata
      901 gcggcggggg cgctgctggg atactggata gtcaagcgca tctcccagga gcgcttcaac
      961 cagatcgtcc tcacgctcgc cttcgtcgcg gcgctgaggc tgctgacgta gcgagctatc
     1021 ttccggagga agccgccgga ggccagagca aaaaaacccc ggagggttgc ggcgtccccc
     1081 tgcgctacct gggttttcac tcggtctacg tcctttgcgt aggcgcgctc ctcatcggga
     1141 aggaattgcc cggccacttc cccgggcatt ttaaaaccgg ggagtattgg gcggacgaat
     1201 cccttcgcaa agagcccctg caacggcacg ctgctcagtc cacgagcatc tcccggatga
     1261 cattcttcac cagaagagcc gtctctccgt ccagacatcc tatctttcga cgtagcctct
     1321 tcctgtctat cgccctgagc tgatcgagaa cgatccaacc tgaaacgtcg cctatcgaaa
     1381 aatgaacgcg ggtaggatag ctttcacgtt gtctgcttgt taccggcgca accagaacgg
     1441 tcagcagatg catgttcatt tccggcggag agacgacaac gcacgggcga gtcttattca
     1501 tctccgctcc ctgcgtcggg tcgagatcgg tccagtacac gccgtactga accaagttct
     1561 cgctat
//
LOCUS       DAOSWW010000731         1566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_70402_length_1566_cov_2.503640, whole genome shotgun sequence.
ACCESSION   DAOSWW010000731 DAOSWW010000000
VERSION     DAOSWW010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70402_length_1566_cov_2.503640"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PK442_12465"
     CDS             <1..721
                     /locus_tag="PK442_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOO88508.1"
                     /translation="REAVTFAALGFLGVFFHNSIQAYALQTVSAGMSGLIIAANPIAI
                     ALLGSLLLSEPLGKAKIAGILLAACGVLVIISRGNISVFLQREYSFGELVMVLSVFSW
                     GFFSVLSRKALQRTSPELGMTYALTFGWLFSIIPFLANGGVAELPSVSGKAWANILFL
                     GILCSALAYLFWYDALQVLPASEVGVFLYVNPVVAVIISALFLGEPVSASMLFGGAMV
                     FSGVWCVNSLSRMQEKRRKME"
     gene            804..>1566
                     /locus_tag="PK442_12470"
     CDS             804..>1566
                     /locus_tag="PK442_12470"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HOO88509.1"
                     /translation="MKILTAPSNGGFVREGALALFVFSKESVSGGLLDEHFADMAARL
                     MEEPSFRGKKGKITRLPLLNGGFSAAYLVGLGDRAKNGKESALHENIRSRSAELLRRC
                     RADGVSKITVVLPDAPSFGTSCALAEGAELGAYAFSKYKSIPEEERLPEPAELLLLDG
                     VDEGVSRGKMFACSQNWAKDIANEPGNKITPGIMADMAVQWGKNLGFEVSVWDEERLL
                     REGMEGIYSVGMGSEHPPRLIHMVYRPSDSVRKVAL"
BASE COUNT          313 a          374 c          470 g          409 t
ORIGIN      
        1 gcgtgaggct gtaacgttcg ccgcgctcgg ttttcttggg gtctttttcc ataactcgat
       61 acaggcctat gcgttgcaga ctgtgtccgc cgggatgtca gggttgatta tcgcagcaaa
      121 tccgatagca atagcgcttc ttggttcact ccttctctcc gaacctcttg gcaaagccaa
      181 aatcgcaggt attctgctgg ctgcgtgcgg agtgctcgtc attatctccc gcggtaatat
      241 ttctgttttt cttcaaaggg agtattcctt cggtgagctc gtcatggtgt tgagcgtctt
      301 ttcgtgggga tttttctcgg tgctttcgcg aaaggccctg cagcggacgt cgcccgagct
      361 cggtatgacc tacgcgctga cgttcgggtg gttgttttcc attattccct ttcttgcgaa
      421 tggcggagtg gccgagcttc cgtcagtttc gggcaaagcg tgggcgaata tcctttttct
      481 tggcattctt tgctctgcgc tggcctatct gttctggtac gatgcgctcc aggtactccc
      541 cgcgtctgaa gtaggcgttt tcctttacgt caatcctgtg gttgcggtga ttatctccgc
      601 gttgttcctt ggagagcctg tgagtgcatc gatgcttttc ggcggagcaa tggtattttc
      661 cggcgtctgg tgcgtgaaca gcctgtcccg aatgcaggaa aaacggcgga aaatggagta
      721 gcgtctcgac cgaataacga gtgaagaact ggagaaggta ttgaggtcga ggattcataa
      781 gctggaaagg agagttgcgt tcaatgaaaa ttcttacagc cccctcaaac gggggctttg
      841 ttcgtgaagg agctttggct ctgttcgtgt tctccaaaga atccgtatcc ggtggtttgc
      901 ttgatgagca ctttgcggat atggccgcac ggctgatgga agagccctct ttcaggggaa
      961 aaaaagggaa aataacgcga ttgccccttc tgaacggggg attttccgcg gcgtaccttg
     1021 tcggtctcgg ggaccgtgca aagaacggca aagagtctgc tcttcatgag aatatccgtt
     1081 cgagaagcgc cgaacttctg cggcggtgca gggccgacgg agtttcgaaa atcactgtcg
     1141 tcctgccgga tgcgccttca ttcgggacga gctgcgctct tgcggaaggc gccgaactgg
     1201 gagcatacgc ttttagtaag tacaagagca ttcccgagga ggaacgactt cccgagcctg
     1261 cggaacttct tctgctcgac ggcgtcgacg aaggcgtgag ccggggcaag atgtttgcct
     1321 gctcacagaa ctgggcgaaa gatatcgcaa acgagcctgg aaataaaatc acaccgggga
     1381 tcatggcgga catggctgtt caatggggga agaatttggg tttcgaagtc tccgtctggg
     1441 acgaagaacg gcttcttcga gaaggaatgg agggaatcta ttccgttggt atgggctcgg
     1501 agcacccgcc caggttgatt cacatggtgt acaggccgtc ggattctgtt cgaaaggtcg
     1561 cgctcg
//
LOCUS       DAOSWW010000732         1564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_70565_length_1564_cov_2.331345, whole genome shotgun sequence.
ACCESSION   DAOSWW010000732 DAOSWW010000000
VERSION     DAOSWW010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70565_length_1564_cov_2.331345"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PK442_12475"
     CDS             complement(<1..1002)
                     /locus_tag="PK442_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88510.1"
                     /translation="MKRIGLFCVFVLLCALFAGAAFASEKVSAYATTEEPLAKELFDA
                     FEKETGIKVDWVRLSSGEAVARLEAEKANPRASIWVGGVGTLHIDAKNKGLTAPYNSR
                     AASSIPVKFHDPERFWIGLYVGPIAFAINTDRAKELNLALPTSWADIVKPEYAKNVRV
                     ANPGTSGTAYNMVTTIIRVFGNDEDKAFDFLKQLDKSIDQYTRSGSAPGKSCAIGEIP
                     VAIGYLHDLIKLKVENAPIEIALPSDGTGFETAAMSLVKDGPDPVNAKKLYDWMLGQT
                     AMDIIAGWYVIPLSSKASPIDTGYSFEKMTLVDQNDVWDAANKARLLERWNKEIGSAR
                     "
     gene            complement(1090..>1564)
                     /locus_tag="PK442_12480"
     CDS             complement(1090..>1564)
                     /locus_tag="PK442_12480"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HOO88511.1"
                     /translation="SGDKLVAAADISGKGPVGGAIEGKVYIMGRPAYSREDETIRMED
                     ADFEEQTNSALAKAAAWLARPALLKEMTERMVFPLQELREQTVRSLAELLREQKIAED
                     VLMEGSVSALSLESLAVGDNGLRLSLSLGGAVSLRYEDESLKRKEKEAKNGGKKN"
BASE COUNT          257 a          451 c          506 g          350 t
ORIGIN      
        1 cctggcggag ccgatctcct tgttccagcg ttccagaaga cgtgccttgt ttgcggcgtc
       61 ccagacgtcg ttctggtcga cgagcgtcat cttctcgaag gagtagccgg tgtcgatcgg
      121 ggaggctttc gagctgagcg ggatgacgta ccatccggcg atgatgtcca tggcggtctg
      181 gccgagcatc cagtcgtaga gcttcttcgc gttgactggg tccggaccgt ccttcacaag
      241 cgacatcgca gcagtctcga agccggtgcc gtccgaggga agggcgatct cgatgggcgc
      301 gttctcgacc ttgagcttga tcagatcgtg caggtagccg atggcgacgg ggatctcgcc
      361 gatcgcgcag ctcttgccgg gggccgagcc ggagcgggtg tactggtcga tgctcttgtc
      421 gagctgcttg aggaagtcga acgccttgtc ctcgtcgttg ccgaagacgc ggatgatcgt
      481 cgtcaccatg ttgtacgccg tgcccgaggt gcctgggttg gcgacgcgga cgttcttcgc
      541 gtactcgggc ttgacgatgt cggcccagga ggtcggcagc gccaggttga gctccttcgc
      601 gcggtcggtg ttgatggcga aggcgattgg tcccacgtag aggccgatcc agaaacgctc
      661 gggatcatgg aacttgacgg ggatcgagga ggccgcgcgg gagttgtacg gcgcggtgag
      721 ccccttgttc ttcgcgtcga tgtggagcgt gcccacgccg ccgacccaga tggacgccct
      781 ggggttggcc ttttcggcct cgaggcgtgc aacggcctcg ccggaggaga gacggaccca
      841 gtcgaccttg atgccggttt ccttctcgaa ggcgtcgaaa agttctttgg cgaggggttc
      901 ttccgttgtg gcgtaggcgc tcaccttttc tgaggcgaac gccgcgccgg cgaagagcgc
      961 gcagagcaga acaaaaacac agaacagacc gattcgtttc atactttcat ctccctttgc
     1021 tgtaatagag gttcactttc cggaaccgtt ataaaacctt tttgagtata tggagtgaga
     1081 ggcgttgtgt caattctttt taccgccatt cttcgcctct ttttccttcc gtttcaatga
     1141 ctcgtcctcg tagcgaagcg aaacggcgcc tccgagggag agggagaggc ggagcccgtt
     1201 atcgcctacc gcgaggcttt caagagagag cgcggagacg gagccctcca tgaggacgtc
     1261 ctccgcaatt ttctgttccc gcagcagttc cgcgagcgac cgtacggtct gttccctcaa
     1321 ctcctggagc gggaagacca ttcgttcggt catctctttg agaagcgcgg ggcgggcgag
     1381 ccacgccgcg gcctttgcaa gggcgctgtt cgtctgttcc tcgaagtcgg cgtcttccat
     1441 ccggatcgtt tcgtcctccc gtgaataggc gggccgcccc atgatgtaga ccttcccctc
     1501 gatagcgcct cccacggggc cttttcccga gatatcggcg gcggcgacga gtttgtcgcc
     1561 gctg
//
LOCUS       DAOSWW010000733         1563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_70610_length_1563_cov_3.303714, whole genome shotgun sequence.
ACCESSION   DAOSWW010000733 DAOSWW010000000
VERSION     DAOSWW010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70610_length_1563_cov_3.303714"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK442_12485"
     CDS             <1..188
                     /locus_tag="PK442_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HOO88512.1"
                     /translation="ALEALHVQGIKTTVEGNDSMRWRLIDAGDLTVHVFSKAGRDHYN
                     LEKMWRDARSYHYAYHD"
     gene            complement(322..1116)
                     /gene="tpiA"
                     /locus_tag="PK442_12490"
     CDS             complement(322..1116)
                     /gene="tpiA"
                     /locus_tag="PK442_12490"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOO88513.1"
                     /translation="MKKIYLYGNWKMNMLPSEAEAFSNELLSETAGELYSTRSVDIAL
                     FPPYISIAPVVRMLPEKSFIKAGAQDGWFEDRGAYTGAVSMDMIREIGCTHVLVGHSE
                     RRSIFGDTDEIVGRKLKQAIQKGLIPVLCFGETLEQREAGQTLAILGKQLLAAWKDIE
                     ADEIKGSDNLPGILLAYEPVWAIGTGRNAKAADAQEACAFSRNLFKERFGKQMDCPVL
                     YGGSVKESNAAELLSQQDIDGALIGGASLKVGSFLGIYENYRKSAD"
     gene            complement(1144..>1563)
                     /gene="pgk"
                     /locus_tag="PK442_12495"
     CDS             complement(1144..>1563)
                     /gene="pgk"
                     /locus_tag="PK442_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOO88514.1"
                     /translation="GVEILLPTDVVVGTSLEMTSGEKIVSADALPDDAMGLDIGPKTV
                     SLFAERIAGARTVLWNGPMGVFENPLFASGTKGVAEGVAACTRKGGITVVGGGDTAAA
                     AKMLGFDQNVSHVSTGGGASLEFCEGKDLPGIKPLEE"
BASE COUNT          363 a          421 c          367 g          412 t
ORIGIN      
        1 cagctctgga ggcgcttcac gtgcagggaa tcaagacaac cgtcgaggga aacgacagta
       61 tgcggtggcg tcttattgac gccggagacc tgacggtaca tgtgttcagt aaagccggga
      121 gagatcacta taacctagag aagatgtgga gagacgcgcg ctcgtatcat tatgcatacc
      181 atgattgaga acgctgcgtt tttttcgttt ctataaagag attcaaaaga gaaccgggca
      241 gaccgaaacg aagtctgccc ggtttttcag ttctcttttt gaggtgtcct ataatgtatc
      301 ttatgttaca tattctcttt ttcagtccgc cgacttccgg tagttttcat aaattccaag
      361 gaacgaaccg accttcaaag aagctcctcc gataagagcg ccgtcgatat cctgctgaga
      421 taaaagctcc gcggcatttg attctttgac gctgcctccg tacagaacgg gacaatccat
      481 ctgcttacca aatcgctctt tgaaaagatt ccgtgaaaaa gcacatgcct cctgagcatc
      541 cgcagccttt gcgttacgtc ctgtgccgat agcccataca ggttcatatg caagcagaat
      601 accgggaagg ttatccgaac ccttgatttc gtccgcttcg atatccttcc atgccgccag
      661 aagctgcttc ccaagaattg cgagcgtttg tccagcttca cgctgttcaa gcgtctctcc
      721 gaaacaaagt acgggtataa gtcctttctg gatggcctgt ttcagttttc gcccgacgat
      781 ctcatctgta tcgccgaata tgctccttct ttcactatga cccacaagta catgcgtgca
      841 ccctatttca cgaatcatat ccatggatac tgcaccggtg taggcgcccc ggtcttcgaa
      901 ccagccgtcc tgagcgcccg ctttgatgaa gcttttctcc gggagcatgc gaactactgg
      961 cgctatcgat atgtagggcg ggaaaagagc gatgtcgacc gaacgtgtgc tatacagttc
     1021 ccccgctgtt tcagaaagaa gttcgtttga aaaagcttcc gcttcggaag gaagcatgtt
     1081 catcttccag ttgccataaa gatagatttt tttcatggta tgctcatcgg cctcctttat
     1141 tgtttactct tcgagcggtt tgattcccgg aaggtctttc ccttcgcaaa actcaagact
     1201 tgccccgcct cctgttgaaa cgtgagagac attctggtcg aagccgagca tttttgccgc
     1261 tgcggcagta tctcctcctc cgacgacggt aatgccacct ttgcgggtgc atgcggccac
     1321 tccttcagcc acccccttcg ttcccgaggc aaagagagga ttttcgaaga cgcccatcgg
     1381 cccgttccag agaacagttc tggctcctgc gattctttcg gcgaaaaggg atacggtctt
     1441 cggtccgata tccaatccca tggcatcgtc aggaagagcg tcggcagaca caatcttttc
     1501 accgctcgtc atttctagag aggttcctac aacaacgtct gtcggtaaga ggatttcgac
     1561 tcc
//
LOCUS       DAOSWW010000734         1562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_70683_length_1562_cov_3.129396, whole genome shotgun sequence.
ACCESSION   DAOSWW010000734 DAOSWW010000000
VERSION     DAOSWW010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70683_length_1562_cov_3.129396"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PK442_12500"
     CDS             <1..461
                     /locus_tag="PK442_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO88515.1"
                     /translation="GLGAAIELTASRMKDASSRVGKLRNMLIDGVMKVIPHAKLNGAP
                     VSERLPNNSNFSFIGIEGETLLLDLDGAGIYASTGSACSSGSLDPSHVLMAIGLSHEQ
                     AHGSLRMTLGEETSEEDIRYVLSVLPAIVERRRAMSPLWEDFLKNTQGGK"
     gene            466..891
                     /gene="nifU"
                     /locus_tag="PK442_12505"
     CDS             466..891
                     /gene="nifU"
                     /locus_tag="PK442_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOO88516.1"
                     /translation="MYSEKVSDHFQNPRNVGEIENPDGVGQAGNPKCGDIMKIYLRVE
                     NEVITDIKFKTFGCASAIASSSMATELVKGRNIDEAWNLTNSAVAEALDGLPPVKMHC
                     SVLAEEAIHAAINDYRRRKGLPVLEGKEYDHEHDHAHQH"
     gene            895..1470
                     /locus_tag="PK442_12510"
     CDS             895..1470
                     /locus_tag="PK442_12510"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOO88517.1"
                     /translation="MNGAFNRCPWPGTDPLYVAYHDEEWGVPVREDATLFEFLLLEGA
                     QAGLSWITVLRKREAYRSAFDGFDPEIIAGYDERKIGELMRNEGIIRNKRKIEGAVKN
                     ARAFLRIRDRKGSFSAYLWDFLDGEPIVNRWKTMEEVPAVTPLAEKVSGCMKKEGFVF
                     FGPVICYAHMQATGMVNDHLTSCFRHPEYGY"
BASE COUNT          370 a          427 c          460 g          305 t
ORIGIN      
        1 taggactcgg cgcggcaatc gaactgactg cgtccaggat gaaggacgcg tcttcccgcg
       61 tcggaaagct ccgtaacatg ctgatcgacg gcgtgatgaa ggttatccct catgcgaagc
      121 tcaacggcgc tccagtaagc gagcggcttc cgaacaactc gaatttcagc tttatcggga
      181 tcgaaggcga aacgcttctt ctcgacctgg acggggccgg tatctacgct tccaccggca
      241 gcgcctgttc ctctggatcg ctggatccgt cgcacgttct catggcgatc ggcctttccc
      301 atgaacaggc gcacggttcg ctccgtatga cgctcgggga ggagacttcg gaagaggata
      361 tccgctatgt tctctccgtg cttccggcaa tagtagagcg ccgccgcgct atgtcgcctc
      421 tctgggaaga ctttctgaag aacacgcaag gagggaaata acgctatgta cagcgaaaaa
      481 gtatcggatc actttcaaaa tccgcgcaac gtcggtgaaa tcgagaatcc ggacggggta
      541 ggccaggcag gaaaccccaa gtgcggcgat atcatgaaaa tatacctccg cgtggagaac
      601 gaagtaatca ccgacattaa gttcaagacg ttcggctgcg cctccgcgat cgcatcttcg
      661 agcatggcga ccgagcttgt gaagggacgg aatatcgacg aagcgtggaa tcttacgaac
      721 agcgcagttg cagaggccct cgacggactg ccccctgtga agatgcactg ttccgtcctc
      781 gcggaagaag ccattcacgc cgcaatcaac gactatcgca gacggaaggg gctgccggtg
      841 ctcgagggta aagagtacga tcacgagcac gatcacgctc atcagcactg agccgtgaac
      901 ggcgctttca atcgctgccc gtggccgggg accgaccccc tctacgtcgc ataccacgat
      961 gaggagtggg gcgttcccgt ccgcgaggac gcgacgcttt ttgaatttct cctattggaa
     1021 ggcgcgcagg ccggtctctc ctggatcact gtcctccgga aacgggaagc gtacaggagc
     1081 gcgttcgacg gttttgatcc ggagataatt gccgggtatg acgaacgaaa aataggcgag
     1141 ctgatgcgga acgagggaat catccggaac aagcggaaga tagagggcgc cgtgaaaaac
     1201 gcacgcgcct tcctccggat cagggatcgg aaaggctctt tttcggcata cttatgggat
     1261 ttcctcgacg gggagccgat cgtcaacaga tggaaaacca tggaagaagt gcctgctgtg
     1321 accccgctcg cggagaaagt aagcgggtgc atgaaaaaag aggggttcgt cttcttcggt
     1381 ccggtgattt gttacgcgca tatgcaggct acgggaatgg tgaacgatca cctgacgagc
     1441 tgctttcgcc atcccgaata cgggtattga agcgggaaaa gatgaacgga aaggaagccc
     1501 cctccgtcat cttccccgct tcttttctca gcctaacgaa gaccttcgaa cgggccacgc
     1561 ca
//
LOCUS       DAOSWW010000735         1562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_70707_length_1562_cov_2.358328, whole genome shotgun sequence.
ACCESSION   DAOSWW010000735 DAOSWW010000000
VERSION     DAOSWW010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70707_length_1562_cov_2.358328"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..498)
                     /gene="gspG"
                     /locus_tag="PK442_12515"
     CDS             complement(61..498)
                     /gene="gspG"
                     /locus_tag="PK442_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOO88518.1"
                     /translation="MTLFFKGKRRKGFTLVEVLVVVVILGLLAALVAPKVVGQGEEAK
                     RTATSVQIREIEQALEMYRLDNSMYPTTAQGLEALVNKPSTPPEPRRYREGGYIRRLP
                     VDPWGSPFVYRMPGDHAEFDLFSKGPDGEEGGEGAGKDITNWE"
     gene            complement(534..749)
                     /locus_tag="PK442_12520"
     CDS             complement(534..749)
                     /locus_tag="PK442_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO88519.1"
                     /translation="MIYILRIGEMGGDLPGAIKQVSDMNWEEALESMERLSTLAEPVL
                     VLFLGFSVGFVVLAILLPIFELSSLAG"
     gene            complement(746..>1562)
                     /locus_tag="PK442_12525"
     CDS             complement(746..>1562)
                     /locus_tag="PK442_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO88520.1"
                     /translation="IIEGAAESVKKGKVFGLAYHSLFCKGLSSYLNRGVPLAEALRFL
                     SRHSNDRRVSAACLHLHESVQGGKRLSAALEETGLFRDDLVRIVDSGERTSALSGVLD
                     QVARQYAMEDKQRRKIRSALTYPVAMSIIGIGVVVFLLTYVVPKLTELFSEMGQQLPL
                     PTRILLAITAFLRSWGLPLLLVLLLVILYIRRRKKKFTLPFFRRVRESITLSLVMSHL
                     STLLSAGIPLVQALNMASAMDPNSQRWLDAARLVKEGFRFDRALRSRAPSQKR"
BASE COUNT          388 a          461 c          384 g          329 t
ORIGIN      
        1 caactgcgca taaaaaaggc cgcctctgtt tcgtgagacg accttgatac acacttctgt
       61 ctattcccag ttcgtaatat cctttcctgc tccttcgccg ccctcttctc catctggtcc
      121 ctttgagaag aggtcaaact cggcatgatc cccgggcatt cgataaacaa acgggcttcc
      181 ccacgggtcg acaggaagac gtcggatata ccctccctcg cgatagcggc gtggttccgg
      241 cggagttgaa ggtttattca caagcgcctc aagcccctgc gccgtggtcg ggtacatact
      301 gttgtcgagc ctgtacattt ccaaggcctg ttcgatctcg cgaatttgaa ccgaagtagc
      361 ggttcgtttc gcctcttccc cctgccccac aaccttcggc gccacgagag cagcaagaag
      421 cccaagaata acaacaacta caagaacttc aacaagagta aatccctttc tccgcttgcc
      481 tttgaaaaac aaagtcataa tatcttcctc catttccttt cattaaattc gtcctaacct
      541 gcaaggctcg atagctcaaa tatcgggaga agaatagcaa ggacgacaaa acccaccgag
      601 aaacccagga aaagcaccag gaccggttca gccagcgttg agagacgttc catactctca
      661 agcgcctcct cccagttcat atccgagacc tgtttaatag cccctggcag gtcgccgccc
      721 atctcgccta tacgaagtat gtagatcacc tcttctgaga aggcgccctg cttctcaagg
      781 cccggtcgaa gcggaatcct tctttgacca aacgggccgc gtccagccat cgttgcgaat
      841 ttggatccat cgcagaggcc atgttgagcg cctgaacaag gggaataccg gctgaaagga
      901 gcgtgctgag atgcgacatc acgagtgaaa gcgtaatgct ctcccgtacc cgccggaaaa
      961 aaggaagggt aaatttcttc tttctgcgcc ggatgtacag aataacgagc agcagcacga
     1021 gcaacagcgg aagcccccat gaacgaagaa acgccgtaat cgccagcagt attctcgtcg
     1081 gaagcggaag ctgttgtccc atttccgaaa aaagttccgt cagcttcggg actacgtagg
     1141 tcaacaggaa aacaaccaca ccgatgccaa taatgctcat ggcaaccgga taggtgagag
     1201 ccgaacggat cttgcgtcgc tgtttgtcct ccatcgcgta ctggcgcgct acctggtcga
     1261 gaactccgct caaggccgag gttcgttccc ccgagtccac aatgcgaaca aggtcgtccc
     1321 ggaagagtcc tgtctcctca agcgccgcgg agagacgttt tcctccctgc acgctctcgt
     1381 gcagatggag acaggccgcc gagacacgac gatcattgga gtggcggctg aggaagcgca
     1441 atgcttccgc gaggggcact cccctgttga ggtaagaaga gagacctttg cagaaaagtg
     1501 agtgatacgc gaggccgaac accttgcctt tctttaccga ttcggccgcc ccttcaatga
     1561 tc
//
LOCUS       DAOSWW010000736         1559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_70944_length_1559_cov_2.611037, whole genome shotgun sequence.
ACCESSION   DAOSWW010000736 DAOSWW010000000
VERSION     DAOSWW010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70944_length_1559_cov_2.611037"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PK442_12530"
     CDS             <1..361
                     /locus_tag="PK442_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88521.1"
                     /translation="NAIGIGLADITTLRAVRGLDFASTYTNGVTAGDIRGCAIPLLAN
                     NERDAITIAISGCAPKTAKECRIVRIRNTLELSSIAVSEAYFDELSGNANIRVLSSPE
                     PMRFSTEGDLERAGASS"
     gene            complement(387..>1559)
                     /locus_tag="PK442_12535"
     CDS             complement(387..>1559)
                     /locus_tag="PK442_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOO88522.1"
                     /translation="LLETNYPKSSVRHLPLRKGVVELGEPIMVLGYPRAFQLGDSITV
                     TKGDISSIRKAGDDTLVQFTAFVAGGSSGSPIIDEKGRVAGIVTSELTLGQGMFLGLS
                     APSIQRYVGEHLPKVEELIVDLPPPPPVIRSPALPAAPRELPPDTSAMVKEAQELLNA
                     LGYDAGAEDGVYGPRTSAAVKAFQRVKKLRRDGHVTKKLLDLLREEKRKTDETRAAAI
                     GREIQSLTENAMRGNPDAQRELGLLYREGRRVPPDYSKAAGWLRMAALHGDVVAQTAL
                     ASMYRYGLGVPKDPVQSASWMTAAAELGHAPAQLALGEMFATAFGVPLDRKRAFGWYR
                     NAADQGYAPAQRALAYAFEHGIGTSADRRQALLWYMRAAEQGDENARNAVGRLRGR"
BASE COUNT          241 a          546 c          501 g          271 t
ORIGIN      
        1 caacgccatc ggcatcggcc tcgcggacat cacaacgctg cgcgccgtcc gggggctgga
       61 cttcgcaagc acctacacga acggcgtcac cgccggggac atccgcggat gcgccatccc
      121 gctactggcg aacaacgagc gcgacgcgat cacgatcgcc atctcgggct gcgctcccaa
      181 gaccgcgaaa gagtgccgga tcgtgcggat acgaaatacg ctcgaacttt cctccatcgc
      241 cgtctccgag gcctacttcg acgagctgtc gggaaacgcg aacatacgcg tgctctcctc
      301 tccggagccg atgcgctttt ccactgaggg cgacctcgag cgggctgggg cctcctcgta
      361 aaagggtgga ggggagactc ctctttctac ctcccgcgca atctgccgac ggcgttgcgg
      421 gcgttttcgt ccccctgctc ggccgccctc atgtaccaga ggagcgcctg ccgtctgtcg
      481 gcggacgtgc cgattccgtg ctcgaaggcg taggcgaggg cgcgctgcgc cggagcgtac
      541 ccctgatccg cagcgttgcg gtaccatccg aacgcccgtt ttctgtccag cggcacgccg
      601 aaggcggtcg cgaacatctc ccccagcgcg agctgcgcgg gcgcgtgccc cagttccgcg
      661 gcggcggtca tccaggaggc ggactgcacg ggatctttcg gtacgccgag gccgtaccgg
      721 tacatggagg caagcgcggt ctgggcgacg acgtcgccgt gcagcgcagc catgcgcagc
      781 cacccggcgg ctttcgaata gtcgggcgga acgcgccgcc cctcgcggta gagcaggccg
      841 agttcgcgct gcgcgtccgg atttccccgc atcgcgttct ccgtgagaga ttgaatctct
      901 ctcccgatgg cggcggcccg cgtctcgtcg gtctttcgtt tctcctcccg caggaggtcg
      961 agcagcttct tcgtcacgtg gccgtcgcgg cggagctttt tcacccgctg gaacgccttc
     1021 acggcggcgg atgtccgcgg accgtacacg ccgtcctcag ctcccgcgtc gtagccgagc
     1081 gcgttcagaa gctcctgcgc ctccttcacc atcgccgagg tgtccggagg aagctcgcgc
     1141 ggggccgcag ggagcgcggg cgaccggatg acgggcggcg ggggaggcag gtcgacgatc
     1201 agctcctcga ccttcggcag atgctcgccg acataccgtt gtatcgacgg cgcgctcagc
     1261 cccaggaaca tcccctgtcc gagggtcagc tcgctggtca cgattccggc gacccgcccc
     1321 ttttcgtcga tgatcggact gccgctcgag ccccccgcca cgaaggcggt gaactgcaca
     1381 agcgtgtcgt cgccggcctt tctgatggag ctgatgtctc ccttcgtcac ggtaatggag
     1441 tctcccagct ggaaagcgcg cggatacccg agaaccatga tcggctcccc gagttcgacg
     1501 acgcccttcc tcagcgggag gtggcgcacc gacgacttcg gatagttcgt ctccagcag
//
LOCUS       DAOSWW010000737         1559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_70958_length_1559_cov_2.349069, whole genome shotgun sequence.
ACCESSION   DAOSWW010000737 DAOSWW010000000
VERSION     DAOSWW010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70958_length_1559_cov_2.349069"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK442_12540"
     CDS             complement(<1..316)
                     /locus_tag="PK442_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88523.1"
                     /translation="MCALRKSCWVLLLAVFVLGAFAPVSVEGAEAPKKIVWRSSGHGP
                     ASDPSQIYHDMLCKAITEASGGRLEIKPFVGGSIVPAYKEVDAVHENVLQLCYTCPMY
                     NLD"
     gene            complement(403..1230)
                     /locus_tag="PK442_12545"
     CDS             complement(403..1230)
                     /locus_tag="PK442_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO88524.1"
                     /translation="MTIITTLIENSPGEHHGLWSEHGISFHVQKDGHSLLFDAGQSEA
                     FIHNAARLRVNLSATDFVVLSHGHYDHSGGFRCLCDYASDFELRIGKGFFEDKYAVRG
                     AAHDFLGNDFDESFLSERGIRYSFASEATEEILPGVYILSNFPRLHDDEIINPRFLLR
                     RNNAFEPDRFDDEILLAVDTPHGLVVLLGCSHPGMKNMLDAARNRLKRPIYAVLGGTH
                     LVESKGEGLERSLAYLNDGDIKIVGVSHCTGKEAVERLRESNAGFFHNVTGSSFIVE"
     gene            complement(1251..>1559)
                     /locus_tag="PK442_12550"
     CDS             complement(1251..>1559)
                     /locus_tag="PK442_12550"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOO88525.1"
                     /translation="AFAASAERGDTAAMIRFDGEFHRLVAKATKNRTLYHLMKTMTKS
                     LPEGWITSLHIPGRVEKTLGEHSSILDAIRGGDPERAEEMMTLHLANALKDIQASMER
                     "
BASE COUNT          342 a          437 c          440 g          340 t
ORIGIN      
        1 tatcgagatt gtacatcggg caggtgtagc agagctgaag cacgttctcg tggactgcgt
       61 cgacctcttt gtaggcagga acgatggagc cgccgacgaa cggcttgatc tccaggcgcc
      121 cgcccgaagc ctcggtgatg gccttgcaga gcatatcatg ataaatctgg gacggatcgg
      181 aggcaggacc gtggccggag gaacgccaca cgatcttttt cggcgcctcg gcgccctcga
      241 cggaaacggg cgcaaaagcg ccaaggacaa ataccgcgag cagcaatacc caacaacttt
      301 ttctcagagc acacatcttt ttacaacctc cttcagatat atgatgtctt acagaaaccg
      361 ccgcgctttt cgaacgcgtc ggcttcggaa agccagaaag acttactcaa cgatgaacga
      421 gctgccggtt acattgtgga agaaaccggc gttcgactcc cggaggcgtt cgacggcttc
      481 ctttcctgtg cagtggctta ctcctacgat tttgatgtct ccgtcgttca gataggcgag
      541 ggaacgttca agcccctccc ctttcgactc cacaagatgc gttccgccga gcaccgcgta
      601 aatcggccgc ttcagccggt tccgtgcagc gtcgagcata ttcttcattc ccgggtggga
      661 acagccaagc agtacgacaa ggccatgcgg cgtatcgacg gcaagaagaa tttcgtcgtc
      721 gaaccggtcc ggttcgaaag cgttgttccg ccgaagaaga aaacgcggat tgatgatctc
      781 gtcgtcgtgg agccgcggaa aattcgagag gatatagacg cccggaagta tctcttcagt
      841 cgcttccgag gcgaaggagt agcggatgcc ccgttcggag agaaaactct cgtcgaagtc
      901 attacccaga aaatcgtggg cggctccccg gacggcgtat ttgtcctcga aaaaaccctt
      961 tcctatacgc agctcgaaat ccgaagcgta atcgcaaaga caacggaaac cgccggagtg
     1021 gtcgtaatga ccgtggctga ggacgacgaa atccgttgcg gagaggttga cgcgcagcct
     1081 ggccgcgtta tggataaacg cctcggactg ccccgcgtcg aacagaaggg agtgcccgtc
     1141 tttttgtacg tggaaggaga taccgtgctc ggaccaaagt ccgtgatgct cgcccggcga
     1201 gttttctatc agcgtggtga tgatcgtcat gacgctcccc cttcgcgctt tcagcgctcc
     1261 atggatgcct ggatatcctt gagggcgttg gcgaggtgca acgtcatcat ttcctccgcc
     1321 cgctcggggt cgccccccct gatcgcatca aggatggacg agtgttctcc aagtgttttt
     1381 tcaactcttc ccggaatatg gaggctggtg atccatcctt cgggaagaga ttttgtcatc
     1441 gttttcatca ggtgatagag cgtccggttc ttcgtagctt ttgcgacgag tctgtgaaac
     1501 tcgccgtcga accgaatcat tgcagcggta tctccccgct ccgcgctcgc ggcaaatgc
//
LOCUS       DAOSWW010000738         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_71024_length_1558_cov_2.689288, whole genome shotgun sequence.
ACCESSION   DAOSWW010000738 DAOSWW010000000
VERSION     DAOSWW010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71024_length_1558_cov_2.689288"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PK442_12555"
     CDS             complement(<1..234)
                     /locus_tag="PK442_12555"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HOO88526.1"
                     /translation="MESFGRICSVIGSDRPGRKVGGLLRPEHLSRALELLIKASSVAV
                     VTGFFVPACGAPETDGPGGAVMLGRALERCGRSA"
     gene            complement(236..1195)
                     /gene="pfkA"
                     /locus_tag="PK442_12560"
     CDS             complement(236..1195)
                     /gene="pfkA"
                     /locus_tag="PK442_12560"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOO88527.1"
                     /translation="MKRIAVLTSGGDSPGMNAAIRAVVRTALYHGMETIGVMKGYEGL
                     MDGECITLDRAAVGGIIHRGGTILRTARSERFKTEAGLRAALVQMENRKVDALVVIGG
                     DGSFRGAEKLCAMGLPVIGIPGTIDNDIAGTDVTIGYDTALNTALSAVQKLRDTASSH
                     DRLFIVEVMGRDAGFLALDVAVSTGAEYVVVPEIPLDIKKMCDHLHYSRKRGKTHSLI
                     ILAEGVMSALDLRNQLQDTGGYDARVTVLGYIQRGGSPSSLDTILASRMGSFAVESLL
                     EGKTGVMVGSVCGAMSLCPLEDAWKAAKELNPELLALVDKLSV"
     gene            complement(1258..1518)
                     /locus_tag="PK442_12565"
     CDS             complement(1258..1518)
                     /locus_tag="PK442_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88528.1"
                     /translation="MNEWTLRIMAIAEKRISRIPNAEKKRILMAIDELRYDPFAHDVI
                     SLSGRREHRLRVGGWRVLLLIDENDRSVTVLSVNARGDAYKK"
BASE COUNT          322 a          547 c          385 g          304 t
ORIGIN      
        1 cgcgcttcgt ccgcatcgtt ccaacgccct gccgagcatc acagctccgc ccggaccgtc
       61 cgtttccgga gctccgcacg cggggacaaa aaacccagtc accacagcca cggaggacgc
      121 ttttatcaac agctccaaag cccgactgag atgctccgga cgaagaagcc ctccgacctt
      181 tcgtccgggc cggtcggaac ctattacgga acaaatgcgg ccgaaactct ccatatcaca
      241 cgctcagctt gtcgacgagc gccagcagct cgggattcag ctctttagcg gccttccagg
      301 cgtcctccag agggcagagc gacatggcgc cgcacaccga gccgaccatg actccggtct
      361 ttccctcgag cagcgactcc accgcgaagg aacccattct ggaggccaga atcgtgtcca
      421 acgacgacgg gcttcctcct cgctgaatat accccagcac cgtcacgcgg gcgtcgtagc
      481 ccccggtgtc ctgaagctga ttgcgcagat ccagcgccga catcactccc tctgcaagga
      541 tgatcagaga gtgcgtcttc ccgcgcttcc tcgagtagtg cagatgatcg cacatctttt
      601 tgatgtccag agggatctcg gggacgacga cgtactccgc cccggtggaa acggcgacgt
      661 cgagcgcgag gaaccccgcg tcgcggccca tgacctcgac gatgaagagc ctgtcgtggc
      721 tgctcgccgt atccctgagc ttctggactg cggagagcgc cgtgttcagc gcggtgtcgt
      781 accctatggt cacatcggtg ccggcgatat cgttatctat tgtcccggga atgccgatca
      841 ccggcagccc catcgcgcac agtttttccg ctcctctgaa agagccgtcg ccgccgatca
      901 ccaccagcgc atccaccttg cggttctcca tctgcacaag cgcagcgcga agccccgctt
      961 ccgtcttgaa ccgctcgctc cgcgccgtgc gcaggatcgt cccgcccctg tggataatcc
     1021 cgccgaccgc ggcccggtcg agcgtgatgc actcgccgtc catcaacccc tcgtatccct
     1081 tcatcactcc tatggtctcc atcccgtgat agagcgccgt acgaaccacc gcacggatgg
     1141 ccgcgttcat acccggggag tctcccccgc tcgtcaacac cgcgattcgt ttcatcataa
     1201 actgttcgtt cctcccgaca ctaaaataca gacacagaaa tagtaccaca gagcacgcta
     1261 ctttttgtac gcatctccgc gagcatttac tgaaagtacc gtaacggaac ggtcattttc
     1321 atcgatcagc agaagcactc tccacccgcc cactctcaga cgatgctcgc gacgccccga
     1381 aagcgaaatc acatcgtgcg caaaaggatc gtaacgaagt tcgtcgatcg ccatgagaat
     1441 gcgcttcttc tccgcattcg gaatccttga gatccgcttc tccgcgatag ccataattcg
     1501 cagcgtccac tcattcactc tcccaaagct ccttcatcgc ttcatcgaaa gagcgata
//
LOCUS       DAOSWW010000739         1556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_71193_length_1556_cov_1.749500, whole genome shotgun sequence.
ACCESSION   DAOSWW010000739 DAOSWW010000000
VERSION     DAOSWW010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71193_length_1556_cov_1.749500"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(501..>1556)
                     /locus_tag="PK442_12570"
     CDS             complement(501..>1556)
                     /locus_tag="PK442_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88529.1"
                     /translation="WICGYAAGPAGRFELADKNGETIASAEPRIPSERFADAVSALAG
                     ALRVRRLEAICEEMEHGEAEDVERSLRMTGLSPEKTDSSGIFRREKRALYRENREREA
                     RKEIGKLILEEALRMGVLCSKTSFVAVHEKAGSVVSRSLVVANALPRGWDAGFAASAR
                     AMSMQGASLTGGFFVPSNAPRMSMRIASMLGSSAPEDMDCFSVCGNSVPPAEEMLISM
                     SMPRLRRAARSGKSASPEGTDPGIEVFNGPLPREEGLLFEGSAGAGVLQGVSLLTRLV
                     LKTGGKDAKKRLEGSLLRLLINGAPVASVALADMIAFGGERPLNVRLREGDKLEVRIS
                     GYQADEDVRISLFLRTT"
BASE COUNT          276 a          506 c          471 g          303 t
ORIGIN      
        1 gagggagttc gggatttcga gggagacgct ctatcaatat ctgagggaga accaaggcgt
       61 cgggggagag tatcaaaaaa ctgattttca ttgaccaaag agggtggagc aagatccgcg
      121 ggctggaagc ctctcgtttc ctgtcgaggg cgacgcctct ccgcctattc gatcattccg
      181 tcctggcgta ccgattcggc atggcggtag cgtgccctct gttcgagggc acgctaccgc
      241 tggaccgggg tctccgtatc cgtcgaccga gtgtgcgatg atcgtcgccg tatggtccga
      301 ccatccgaag cggtagaagg tgttttcgtc ctccgtcggt accagggcgt gtccgagcgt
      361 aagggaatag cagtccacga tgtcttggca ctcatcagtg aataaaaagg tatcggttgc
      421 ccctccgcct ttggcggggg gcaacttcga caaacactat cggaaacgac ggcggagcag
      481 cgcctccttt cgtcgccgcc ttacgtcgtc ctgagaaaga gcgagattcg cacatcctcg
      541 tccgcctgat accccgagat gcgcacctcc agtttgtccc cttccctcag tcgtacgttc
      601 agggggcgtt cgcctccgaa ggcgatcatg tcggcgaggg cgaccgaagc gacgggggcg
      661 ccgttgatca gcagacgcag cagactccct tcgaggcgct tcttcgcgtc cttcccgccg
      721 gtcttcagta cgaggcgcgt cagcagggag accccttgca gaacgccggc cccggcgctt
      781 ccctcgaaga gaagcccctc ttcgcgaggc agcggtccgt tgaagacctc gattcccggg
      841 tccgtaccct ccggggacgc gctcttcccg gagcgcgccg ctcttcttaa acgcggcatg
      901 ctcatggaga tcagcatttc ctcggcggga ggaaccgaat tcccgcacac cgagaagcag
      961 tccatatcct ccggagcgga gcttcctagc atggaggcga tgcgcatgga catccgcgga
     1021 gcgttggaag gcacgaagaa tcctccggtc agcgatgctc cctgcatgct catcgccctt
     1081 gcgcttgcgg cgaagcccgc gtcccaccct ctggggagcg cgttggcgac gaccagcgag
     1141 cgcgatacga cactcccagc cttttcgtgc acggcgacga aggaggtctt cgaacagagc
     1201 acccccattc ggagcgcttc ctcgaggatc agtttcccga tctcttttcg cgcctcccgc
     1261 tccctgttct cgcggtagag cgccctcttt tcccgacgga agataccgga ggaatccgtc
     1321 ttctcggggg aaagcccggt catgcggagg cttcgttcga catcctccgc ctccccgtgc
     1381 tccatctcct cgcaaatggc ctccagcctg cggacgcgca gcgccccggc gagcgccgag
     1441 acggcatcgg cgaagcgttc ggagggtatg cgcggctccg ccgaagcgat cgtctccccg
     1501 tttttgtccg caagttcgaa ccggcctgcg gggccggcgg catatccgca gatcca
//
LOCUS       DAOSWW010000740         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_71300_length_1554_cov_2.910607, whole genome shotgun sequence.
ACCESSION   DAOSWW010000740 DAOSWW010000000
VERSION     DAOSWW010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71300_length_1554_cov_2.910607"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK442_12575"
     CDS             <1..350
                     /locus_tag="PK442_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88530.1"
                     /translation="RSYEMSGRYGYISLEGGIRHFLEGVEADQFRMDGTWFHPFAKKW
                     GVHSTFSVIEGFSISSAPGRNGFSLWKAGAGVVYSPTDQTDIMVRNIWDFAGKNTGDG
                     SNLELVFSTRIGR"
     gene            357..>1554
                     /locus_tag="PK442_12580"
     CDS             357..>1554
                     /locus_tag="PK442_12580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO88531.1"
                     /translation="MRLGEILLQEGIVDVQTLERALSIQQKSGAQIGDVLIALGKINP
                     LQLAKALSLQFNLPYRNAVDYPANPETLKEVVLVVGEQFLRTNAILPLGRKGNALGVV
                     TSRPNAKEPLEALAKAMNAELFLFVCSEREIQTALTNQFRDSYTEESTMGLFFRNPEE
                     SAMETFTTLQLVLLYALVTLFLFGMLFRGRETLLVLFFLINVFYIFSLGFKLLASILG
                     ARTEILEQVSDEEAAALDPKTLPVYTILLPMYKEPEVVKHLIAGIMEMDYPLEKLDVK
                     ILLEEEDTVTYDAVRALRPPANFEIVRVPYSVPKTKPKACNYGLLFARGEYLTIYDAE
                     DVPEKDQLRKVVAAFRKGPENMICVQAALNYFNSDQNLLTRMFTLEYSYWFDYMLPGI
                     EALGMPI"
BASE COUNT          329 a          440 c          447 g          338 t
ORIGIN      
        1 gcagatccta tgagatgagc ggccgctacg ggtatatcag cctcgaaggc ggaatacggc
       61 actttctcga aggagtcgag gcggatcagt tccgcatgga cggtacgtgg ttccacccct
      121 tcgccaagaa atggggcgtg cattcgacgt tcagcgtcat cgagggtttt tccatcagca
      181 gcgctccggg gcggaacgga ttctcgctct ggaaggccgg ggcgggcgtt gtctattccc
      241 ccacagacca gaccgacata atggtgcgga atatctggga ttttgcggga aagaacaccg
      301 gagacggctc gaacctggaa ctcgtcttca gtacgcgtat cgggagatag cacgtcgtgc
      361 gcttgggtga aattttactt caggaaggga tcgtcgatgt gcagactttg gaacgcgcgc
      421 tctcgataca gcaaaagagc ggcgcgcaga taggagatgt gctgatagcc ctcggcaaaa
      481 tcaaccctct tcagttggcg aaggcgctct cgttgcagtt caacctcccc taccggaacg
      541 ccgtggacta tccggcgaac cccgaaacgc tcaaagaggt tgtcctggta gtgggggagc
      601 aattccttcg gacgaacgcc attctgccct tgggaagaaa agggaatgct cttggcgtcg
      661 taacctcgcg ccccaacgcg aaggagcctc ttgaggcgct cgcgaaggct atgaacgccg
      721 agctctttct cttcgtctgc agtgagcggg aaattcagac ggcgctgacg aaccagttcc
      781 gtgactcgta caccgaagag agcactatgg gactcttctt ccggaatccg gaggagtccg
      841 ccatggagac cttcactacg ctccagcttg tcctgttgta cgccctggtt acgctcttcc
      901 ttttcggcat gcttttccgc gggcgcgaga cgttgctcgt gctctttttc ctcatcaacg
      961 tcttctacat cttttcgctg gggttcaagc tgctcgcctc cattttgggc gcgcgaacgg
     1021 agatactgga acaggtgagc gacgaagagg ccgctgcgct cgatccgaag acgctgccgg
     1081 tctatacgat tctgcttccg atgtataaag agcccgaagt ggtcaaacat ctgatcgccg
     1141 gaatcatgga gatggactac cctctggaga agctcgacgt gaagattctt ctcgaagagg
     1201 aggatacggt cacctacgac gctgtccgcg ctctgcgtcc gcctgcgaat ttcgaaattg
     1261 tccgcgtgcc ctactccgtt cccaagacaa aaccgaaggc ctgcaactac ggcctgctct
     1321 ttgcgcgcgg cgagtatctc accatctacg acgccgaaga cgttcccgag aaggaccagc
     1381 tccggaaggt cgtcgcggcc ttccgtaaag ggcctgagaa catgatatgc gtccaggcgg
     1441 cgctgaacta cttcaactcc gaccagaatc tgttgacgag gatgttcacg ctggagtact
     1501 cctactggtt cgactatatg ttgcccggca tcgaagcgct ggggatgccc atcc
//
LOCUS       DAOSWW010000741         1549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_71683_length_1549_cov_2.784471, whole genome shotgun sequence.
ACCESSION   DAOSWW010000741 DAOSWW010000000
VERSION     DAOSWW010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71683_length_1549_cov_2.784471"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PK442_12585"
     CDS             complement(<1..384)
                     /locus_tag="PK442_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOO88532.1"
                     /translation="MKRALLLVLAFCIVLGASAAIAAEYPSKAVTIIVASNAGGGTDT
                     MARLFAKFAEKYFPQPFVINNIDGAGGQRGFEALARAKKDGYTIGTLYTPHLTAHMSA
                     KRASYTLDDFAMLYNLVTDPGVLVVP"
     gene            complement(493..1239)
                     /gene="phoU"
                     /locus_tag="PK442_12590"
     CDS             complement(493..1239)
                     /gene="phoU"
                     /locus_tag="PK442_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOO88533.1"
                     /translation="MFGKKNGKVESLLYSGDREKILAMVREMGSMASEAFRLAVQSLE
                     ERDDALAERVIEEDDAIDDLEATIDHECLCSIAMRQPVREDLRFVFAVLKIITDLERI
                     GDQGVNIAKKARVLNRYPLLKPLVDIPKMKVIATEMVADSLKAFEENDTLLAEEICMR
                     DEELDKLYDCIFDELIGILGKKPEGDQATAQCAAGLLWVARHLARIGDHATNVAERVY
                     FMVKGERLKPLIEAKKKALQAKFEENGFPH"
BASE COUNT          310 a          440 c          426 g          373 t
ORIGIN      
        1 ggggaccacc agtacgccgg ggtcggtgac aaggttatac agcatcgcga aatcgtcaag
       61 agtgtagctc gcgcgtttgg cggacatatg cgcggtaagg tgcggggtgt acagtgtgcc
      121 gatggtgtag ccgtcctttt tcgcgcgtgc cagtgcttca aaacctcgct gtccgccggc
      181 gccgtcgata ttgttgatga cgaagggctg cgggaaatac ttctccgcga acttggcgaa
      241 gaggcgggcc atcgtgtcgg tgcctcctcc ggcgttggat gcgacgatga tggtcaccgc
      301 tttgctggga tactccgcgg cgattgccgc agacgctcca aggacgatgc agaacgcaag
      361 aacaagcaga agagctcttt tcattttctc gtctcctttc aaatgtgtga ttcacctgtt
      421 gaagcagtag cttttgaaac agaggggaaa ccgctccacc tcctttcaat cgaagacaag
      481 ggcgggaaga cctcagtgcg ggaagccgtt ctcttcgaac ttcgcctgca gggccttttt
      541 cttcgcttcg atgaggggtt tcagccgctc ccccttcacc atgaagtaga ctctctccgc
      601 gacgttggtc gcatggtcgc ctatacgggc gaggtgacgc gcgacccaga gcagccccgc
      661 ggcgcattgg gctgtcgcct ggtcgccctc cggttttttc cccaggatac cgatgagttc
      721 gtcgaatatg cagtcgtaca gcttgtccag ctcctcgtcc ctcatgcaga tctcttcggc
      781 gagaagcgta tcgttctcct caaaggcctt gagcgagtcg gccaccattt ccgtcgcaat
      841 gactttcatc ttcggtatgt ccacgagcgg cttcaacaga gggtaccggt tgagcaccct
      901 cgccttcttc gcgatgttga ctccctggtc gccgatccgc tccaggtcgg tgatgatctt
      961 caaaaccgcg aagacgaagc ggagatcctc gcggacaggc tgacgcatgg cgatcgagca
     1021 caaacactca tggtcgatcg tcgcctccag gtcgtctatt gcgtcgtctt cttcgataac
     1081 cctctccgca agcgcgtcgt cgcgctcttc gagcgactgg acagcgagtc tgaacgcctc
     1141 gctcgccata ctgcccatct cgcggaccat ggcgagaatc ttctctctgt ctcctgaata
     1201 gagaagggat tcgaccttcc cgttcttctt tccgaacaca ctccacccct cctcgaaata
     1261 catcaacggc cccgttgtat ttcccttgtc ggagaaggac tcgtacggga agcgtcaacc
     1321 ggggcaatgt ttttgtaacg ataatgttac aggatacgat atgccttccg gaaagtcaat
     1381 tataatgaag cggccgtgcg ctcccgttca acgttttttt cacgggaatt cgttttctgc
     1441 gcggcgaatc tgctattata catttcaaga tgtagagact tttttcccgg aggagagagg
     1501 gactatggaa aagacttttg tacgatgcat catccgcgga gaggagcgg
//
LOCUS       DAOSWW010000742         1544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_72079_length_1544_cov_2.286098, whole genome shotgun sequence.
ACCESSION   DAOSWW010000742 DAOSWW010000000
VERSION     DAOSWW010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72079_length_1544_cov_2.286098"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PK442_12595"
     CDS             <1..328
                     /locus_tag="PK442_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88534.1"
                     /translation="VDLSDRMDHFPSQLSGGQQQRVAIARAIAKRPEVLLCDEPTGAL
                     DVHTGITVLEAIERVNRELGTLAVIITHNAAIAAMADRVLSLSAGRILSIHSNEKRAP
                     VNTLSW"
     gene            341..>1544
                     /locus_tag="PK442_12600"
     CDS             341..>1544
                     /locus_tag="PK442_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO88535.1"
                     /translation="MLDLKLLRDIRGMKGQMTAIALVMACGLSLMIMSRGLLLSLETT
                     KNSYYTESRFADLFCDLKRAPLFLEPALAMIDGVAQVETSVRGAIILDIPGMKEPADG
                     FVHSLPEGRPRRLNRIHLRSGRLPEAGNRDEILVSDSFAKAHGFRPGDTIDATVYGVR
                     RSFRICGVAISPEYVYEIRPGGMMPDPERFGVFWMSERELSRALGLEGAFNSVAFRLA
                     PGADAGMVKADIDRLLEPYGGLSAYDRERHVSAKLMEDEVAQLRVFSVAFPVVFLSIA
                     AFMVSAAMTRLVKLQREQIAQMKAFGYSGFSVASHYVKFAFVPVVAATLLSGTLGMWA
                     AHAFIGLYQRFFNFPALIFRPDWSALLVALLASGLVSFLGVLGAVGQAAALPPAEAMR
                     PEAPADYSP"
BASE COUNT          248 a          489 c          503 g          304 t
ORIGIN      
        1 cgtcgacctt tcggacagga tggaccactt cccctcgcag ctctccggcg gacagcagca
       61 gcgggtcgcc atcgctcgcg ccatcgctaa acgtccggag gtgctcctct gcgacgaacc
      121 gacgggggcg ctggacgtgc acaccggcat taccgtgctg gaggcgatcg agcgggtgaa
      181 cagggagctg gggacgctcg ccgtcatcat cacgcacaac gcggcgatcg cggcgatggc
      241 cgaccgggtg ctctctctct cggcggggcg tattctctcc attcattcga acgagaagcg
      301 cgcgccggtg aacacgctct cctggtgatg tgtaatgagg atgcttgatc tcaaactgct
      361 cagggatatc cgcggaatga aggggcagat gaccgccatc gccctggtga tggcgtgcgg
      421 tctgtccctg atgatcatgt cgcgtggact gcttctcagc cttgaaacga cgaagaacag
      481 ctactacacg gaatcgcgct tcgcggatct cttctgcgat ctcaagcgcg ctcccctgtt
      541 cctcgagccc gccctcgcga tgatcgacgg cgtggcgcag gtggaaacga gcgtcagggg
      601 agcgatcatc ctcgatattc ccggaatgaa ggagcctgcg gacggcttcg tccactcgct
      661 tccggaggga cgcccccggc ggctcaaccg cattcatctg cgctcggggc gtcttcccga
      721 ggcgggaaac agggacgaga ttctcgtcag cgactccttt gccaaagccc acggcttccg
      781 ccccggagat acgatcgacg ccaccgtcta cggcgtgcgg cgcagcttcc gtatctgcgg
      841 cgtcgccatc tcccccgagt acgtctacga gatcagaccg ggaggcatga tgcccgaccc
      901 ggagcgcttc ggggtattct ggatgagcga acgcgagctc tcgcgggcgt tggggcttga
      961 gggcgccttc aacagcgttg ccttccggct tgcgccggga gcggacgccg ggatggtgaa
     1021 agcggacata gacaggcttc ttgagccgta cggaggactg tcggcgtacg accgtgaacg
     1081 gcacgtctcg gcaaagctga tggaggacga ggttgcccag ctcagagtgt tttctgtggc
     1141 gtttcccgtc gttttcctca gcatcgccgc gtttatggtg agcgcggcga tgacccgtct
     1201 cgtcaagctg caacgcgagc agatagcgca gatgaaggcg ttcggatact ccggcttttc
     1261 cgtggcctcg cactacgtga agttcgcctt tgtcccggtg gtcgccgcaa cgctgctcag
     1321 cggcacgctc ggcatgtggg cggcccacgc cttcatcggg ctctaccagc gctttttcaa
     1381 cttccctgcg ttgatcttcc gtcccgactg gagcgccttg ctcgtcgcgc tgctggcgag
     1441 cgggttggta tcgttcctcg gggtgctcgg tgcggtcggg caggcggcgg cgctccctcc
     1501 cgcggaggcg atgcggccgg aggctcccgc ggattactcc ccgt
//
LOCUS       DAOSWW010000743         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_72140_length_1543_cov_3.327285, whole genome shotgun sequence.
ACCESSION   DAOSWW010000743 DAOSWW010000000
VERSION     DAOSWW010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72140_length_1543_cov_3.327285"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1274)
                     /locus_tag="PK442_12605"
     CDS             complement(129..1274)
                     /locus_tag="PK442_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOO88536.1"
                     /translation="MGLESRVHSGVHFMQGNYAAVEGAVAAGCDFFAGYPITPANEVS
                     EGMARRLPAVGGAFFQGEDELCSIYAITAASLAGAKAMTATASAGYDYMQEGIEYAVA
                     VEAPIVVLDVMRCRGENFATQADIMQVRWAAAGDHEMIVLAPSTVQEMFDFTVRAFNL
                     AEEYRTPVIVISEMSLALMRERLEIPDASTIPVVNRKRTTKHPSEYLPFKAESEFGVP
                     DFAELGTGYRVIHSINPHDERGDIEWEPHAFERLYKRITGKIRENRKKISTTQRFMLD
                     DAEIALIAFGSEVRPCLDAVHAAREKGIKAGVLKLDAPWPIPEEAIAETAAGANAVVT
                     VEMNIGRYAGEVERIVCGKCRTARATKNLGTPHTPDEILSVIEEVRS"
BASE COUNT          300 a          492 c          438 g          313 t
ORIGIN      
        1 ctcgcacgcg cggagaaagg cgctgacaac ctgtccgcag ccgcagccgg ggcagaagaa
       61 atgaggaagt ttgtcctgcc gcatgtactt gagcagcgga ttgccgtttg tatgcatttc
      121 gggagcgctc atgaccgcac ctcctcgatg acggagagaa tttcatccgg agtgtgcggc
      181 gttccgaggt tcttggtcgc ccgcgcggtg cggcactttc cgcacacgat gcgctccacc
      241 tctccggcgt acctgccgat gttcatttcg accgtcacaa cagcgtttgc gccggccgcc
      301 gtctcggcaa tcgcttcctc ggggatcggc cacggcgcat cgagcttcag gaccccggcc
      361 ttgatccctt tctcgcgcgc agcatgaaca gcgtcgagac aggggcgaac ctcgctgccg
      421 aaggcgatga gagcaatctc cgcgtcgtcg agcatgaaac gctgcgtggt tgagatcttc
      481 tttctgttct cccggatctt cccggtaatt cttttataga ggcgttcgaa ggcgtgcggc
      541 tcccattcga tatccccgcg ctcgtcgtgc gggttgatcg agtgaatgac ccggtacccc
      601 gtccccagct cggcgaagtc gggcacgccg aactcgctct ccgccttgaa gggcagatac
      661 tcggaggggt gtttcgtcgt ccgtttgcgg ttcacgaccg ggatagtcga agcatccgga
      721 atctcaagcc gttcgcgcat cagcgccagc gacatctccg agattacaat gaccggcgtc
      781 cggtactcct cggcgaggtt gaacgcacgg accgtgaagt cgaacatctc ctggacggtc
      841 gacggcgcga gcacgatcat ctcgtgatcc ccggccgcag cccaccgcac ctgcatgatg
      901 tcggcctgcg tggcgaagtt ttcgccccgg cagcgcatca cgtcgagcac cactatgggc
      961 gcctccaccg cgacggcgta ctcgatcccc tcctgcatat agtcgtaccc cgcgctcgcg
     1021 gtcgccgtca tcgccttggc cccggcaagg gacgcggcgg tgattgcgta aatcgaacac
     1081 agttcgtcct caccctggaa aaaagcgcct cccacggcgg gaagtcgtct tgccattccc
     1141 tcggagactt cgttcgcagg ggtaatggga tagcctgcga aaaaatcgca cccggcggca
     1201 acggcccctt ccactgcagc gtagttcccc tgcatgaaat ggacgccgga gtgaactcgg
     1261 ctctcaagtc ccattcatac acctccattg tgtatgcccg cgcacagcgg gggcagaatg
     1321 tatcatgtac acgagattat catgcaaaaa atggaatgtc aaatatattt tcttctgctc
     1381 gctgaaaaaa accctcccgt cagggagggc tcgacgacca gcgcaagcgc tttcggagct
     1441 acaggtcgag atactgcaac atttttttcc cgatgacctt ctccacgggc tcgaagagat
     1501 ccggagagcg catccccgct ccaagaagaa gccgtctcct gga
//
LOCUS       DAOSWW010000744         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_72172_length_1543_cov_2.376344, whole genome shotgun sequence.
ACCESSION   DAOSWW010000744 DAOSWW010000000
VERSION     DAOSWW010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72172_length_1543_cov_2.376344"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PK442_12610"
     CDS             complement(<1..352)
                     /locus_tag="PK442_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88537.1"
                     /translation="MNPNAKTIVEVNNLKKYFHTKKGMLHAVDDVSFTINKGETLGLV
                     GESGCGKSTLGRVMIRLLEATSGEVKFWGNDILKYSSSKMKEMRKRVQIVFQDPYSSL
                     NPRLAVSELIAEPLL"
     gene            complement(349..1362)
                     /locus_tag="PK442_12615"
     CDS             complement(349..1362)
                     /locus_tag="PK442_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO88538.1"
                     /translation="MENKKLLLDIQDLSIQYDTEGGTVHAVEHLNLTLEHGENLGFVG
                     ETGAGKTTTALGIMGLIPNPPGIIADGKIFFEGEDLLKKSERQMRDIRGGKIAMIFQD
                     PMTSLNPVMTVDKQIAEMVLLHNDVSEQQALERAVEMLEMVGIKKERAHDFPHQFSGG
                     MKQRVVIAIALACNPILLIADEPTTALDVTIQAQVLELMHELRRKINTSLIMITHDLG
                     IVAEMCDKVAIMYAGRVVEYASTEALYNRPAHPYTEGLFNSIPDIDRDEEELKVIQGL
                     LPDPTNLPSGCSFHPRCPYAIDSCSTDMPPMVEIEAGHFVACPVRFEHWKNAADGNGG
                     VKR"
     gene            complement(1381..>1543)
                     /locus_tag="PK442_12620"
     CDS             complement(1381..>1543)
                     /locus_tag="PK442_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO88539.1"
                     /translation="TPEWGAMLSGGREYTRDYAYMTIFPGLAIMITILALNFLGDGLR
                     DVLDPKLKR"
BASE COUNT          286 a          459 c          435 g          363 t
ORIGIN      
        1 ccagcagagg ttccgcgata agttccgaga cagcgagcct gggattcaga cttgaatacg
       61 ggtcctggaa gacgatctgc acgcgcttgc gcatctcctt catcttcgag gaagaatact
      121 tgagaatgtc atttccccaa aacttcacct cgccgctcgt cgcttccagg aggcggatca
      181 ttacgcgccc aagcgtggac ttgccgcagc cggattcgcc gacgagaccg agcgtctctc
      241 ccttgttgat agtgaacgag acgtcgtcca cagcgtggag catccccttc ttggtgtgga
      301 agtacttctt cagattgttg acttcgacga tcgtcttcgc gttcggattc atcgtttcac
      361 tcccccgttc ccgtcggcgg cgtttttcca gtgctcgaaa cgcaccggac acgccacgaa
      421 gtgccccgct tcaatctcca ccatcggcgg catatccgtc gaacaggagt cgatcgcata
      481 cggacagcga ggatggaacg aacaaccgct tggcagattc gtcggatcgg gcagcagccc
      541 ctggatcacc ttcagctcct cctcgtcgcg gtcgatgtcg ggaatcgagt tgaacaggcc
      601 ttcggtgtag ggatgggccg ggcggttgta cagagcctcc gtggaagcgt actccaccac
      661 tcttcccgcg tacatgatcg ccaccttgtc gcacatttcg gcgacgatgc ccagatcgtg
      721 agtgatcatg atgagcgacg tgttgatctt gcgccgcagt tcgtgcatca gttcgagcac
      781 ctgcgcctgg atggtgacgt cgagcgccgt cgtgggttcg tcggcgatga ggaggatggg
      841 gttgcacgcc agcgcgatgg cgatcaccac gcgctgcttc atcccgccgc tgaactggtg
      901 cgggaagtcg tgcgcccgtt cttttttgat gccgaccatt tcaagcatct ccacggcccg
      961 ttcaagcgct tgctgctcgc tgacgtcgtt gtggaggagc accatctccg cgatctgctt
     1021 atccaccgtc atgaccggat tcagcgaagt cataggatcc tggaagatca tcgcgatctt
     1081 gccgccgcgg atatcgcgca tctggcgttc gctctttttt aagaggtctt cgccctcgaa
     1141 aaagatcttc ccgtccgcga ttatgcccgg aggattgggg atcagcccca tgataccgag
     1201 cgccgtagtt gttttcccgg cgcccgtttc gcctacaaaa ccaaggtttt cgccgtgttc
     1261 gagcgtgaga ttgagatgtt cgacggcgtg caccgtgccg ccttccgtat cgtactgaat
     1321 gctcagatcc tgtatgtcca gaagtaattt tttgttttcc atagtcgtcg cctccgcctt
     1381 ctatcgcttc agcttcgggt cgagcacgtc gcgcagaccg tcgccgagaa aattcagcgc
     1441 caggatggta atcatgatgg cgaggccggg gaaaatcgtc atatatgcgt agtcacgggt
     1501 gtactcgcgt ccccccgaga gcatggcgcc ccactctggt gtg
//
LOCUS       DAOSWW010000745         1542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_72225_length_1542_cov_3.336247, whole genome shotgun sequence.
ACCESSION   DAOSWW010000745 DAOSWW010000000
VERSION     DAOSWW010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72225_length_1542_cov_3.336247"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PK442_12625"
     CDS             complement(<1..274)
                     /locus_tag="PK442_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88540.1"
                     /translation="MNRRGSNQLREEAGERSARQICVFQQIIATRQKWTTRRLFTLDC
                     PIRVLTKATIMGILNQRLTFVEFFAFLSLFKGGGWRGRAPCVKNAHS"
     gene            461..>1542
                     /locus_tag="PK442_12630"
     CDS             461..>1542
                     /locus_tag="PK442_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO88541.1"
                     /translation="MPENILETGFDDELKKSYLSYAMSVIADRALPDARDGLKPVQRR
                     ILHAMKELGLSSGGSHKKSARVVGETMGKYHPHGDSSIYEAMARMAQTFSLRVPLVDG
                     QGNFGSLDGDSPAAMRYTEVRLAPEGELMHADVDEETVNFVPNFDESLTEPSVLPAVL
                     PNLLINGASGIAVGMATNIPPHNPVETLSLLSEYLRDGCSWSVSETASRMPGPDFPTG
                     GEIVGTTGIREMYETGRGKFTLRGKTHIEETKNRYLVVVTEIPYGATKSRIVAQIAEK
                     LDEKNVEGIVTVRDESDRTGDRVVVEMQKKTDPKAVEALMHSATQLSGTFGGNLLALA
                     GGAPRTMTLKDIFDCFVGHRREIVRR"
BASE COUNT          326 a          471 c          466 g          279 t
ORIGIN      
        1 tggaatgcgc attcttcacg caaggagctc tgccgcgcca accacctcct ttaaataaag
       61 acaggaaagc aaaaaactcc acgaaggtca gccgttggtt cagaataccc ataatagtag
      121 cttttgtcaa tactcttatg gggcaatcta gtgtgaacag ccttctggtt gtccattttt
      181 gccgtgttgc tataatttgc tgaaagacac atatttgtcg tgcagagcgc tctcctgcct
      241 cttcccttag ctggttgctt cctcgtctgt tcattgcgtt ttcccctcgg accgaaggct
      301 cgacggaggg ggaaaaggcg ctctgtttgc agaaaaaagg ggtggtcggg tacaatagct
      361 gtctgaacgc cggaaccttc ccgtgagaag atcgcgtccg gcggcgggaa tccgcacatg
      421 aacgcattcc gccgacacct caagaacaag gagaccatcc atgcctgaaa atatactcga
      481 aactgggttc gacgacgaac tcaaaaaaag ctatctctcc tacgcgatgt cggtcatcgc
      541 cgaccgcgcg ctcccggacg ccagagacgg tttgaaaccg gttcagcgcc gcattctcca
      601 cgcgatgaag gagctcggcc tctcctccgg gggatcgcac aaaaagtcgg cgagggtcgt
      661 cggcgagacg atgggcaagt accatccgca cggcgactcc tcgatatacg aggccatggc
      721 gcgcatggcg cagacctttt cgctccgcgt cccgctggtg gacggacagg gaaacttcgg
      781 ctcgctcgac ggcgactctc ccgcggcgat gcgctacacg gaggtccgcc tggcgccaga
      841 gggagagctg atgcacgccg atgtcgacga ggagacggtg aacttcgtcc cgaacttcga
      901 cgagtcgctc accgagccct ccgtccttcc cgccgttctt ccgaacctgc tgatcaacgg
      961 ggcgtcggga atagcggtcg gcatggcgac gaacatcccg ccgcacaacc ccgtcgagac
     1021 gctctccctc ctctcggagt accttcggga cggctgctcg tggagcgtct ccgagacggc
     1081 gtcgaggatg cccgggcccg acttccccac ggggggcgag atcgtcggga cgaccggtat
     1141 ccgcgagatg tacgagacgg ggcgcggcaa gttcacgctt cgcggcaaga cgcatataga
     1201 agaaacgaag aaccgctacc tcgtcgtggt caccgaaatt ccgtacggcg ccacgaagtc
     1261 gcgcatcgtc gcgcagatcg ccgagaagct cgacgagaag aacgtcgagg ggatcgtgac
     1321 cgtccgggac gagtccgaca ggacgggcga ccgggtggtc gtggagatgc agaagaaaac
     1381 cgatcccaag gccgtggagg cgctgatgca ctcggccacg cagctctccg gcaccttcgg
     1441 cggcaacctg ctcgcgctcg cggggggcgc gccgcggacg atgacgctca aggatatctt
     1501 cgactgcttc gtcggccacc ggcgcgagat cgtccggcga ag
//
LOCUS       DAOSWW010000746         1541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_72291_length_1541_cov_4.067295, whole genome shotgun sequence.
ACCESSION   DAOSWW010000746 DAOSWW010000000
VERSION     DAOSWW010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72291_length_1541_cov_4.067295"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PK442_12635"
     CDS             <1..436
                     /locus_tag="PK442_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOO88542.1"
                     /translation="ELINHCRNMRIGEILEQPDVKERVELYFEQTRRFTEMVHTHTKV
                     EQNVIVTDLRDVSPIYTGNRFLIYSLYPEQNVSVWIVDGRQKQNVSIACGYSILNRTC
                     GVDIGALMLKHGGGGHKMVGTCQVPYSEADDRIREIVDALKG"
     gene            complement(514..1023)
                     /locus_tag="PK442_12640"
     CDS             complement(514..1023)
                     /locus_tag="PK442_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOO88543.1"
                     /translation="MESWKGCAYTALIVFGYLVRGNKVLIVRRANDPYKGQRTVPGGR
                     KQRDETLREALVREIEEETGCRVRSMEYAGMLHAIVEGRDMEYLSVYFISRDFDGEPR
                     DSEEGTVEWMDIDESLTMEDMHPIYLRLVPYILKGNYPMEGAMRISADDASEYMLFGP
                     GEHPAPEAR"
     gene            complement(1082..>1541)
                     /locus_tag="PK442_12645"
     CDS             complement(1082..>1541)
                     /locus_tag="PK442_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOO88544.1"
                     /translation="NVSLRGDRIVTFREVHLGVAVALEGSLIVPVIRNADEKSLTRIS
                     RELKDLSRRARENALAPEEISGGTFTLTNLGGYRSTEFFTPVINQPESAILGVGRTND
                     MPVAMDGEVAVRPMTALSLAHDHRVIDGAPAAEFLALLIELIEHPFRVFL"
BASE COUNT          274 a          538 c          454 g          275 t
ORIGIN      
        1 ggagctgatc aaccactgcc ggaacatgcg catcggcgag atcctcgaac agccggacgt
       61 gaaggagcgc gtggagctct acttcgagca gacgcggcgt tttacggaga tggtccatac
      121 acacacgaag gtggaacaga acgtcatcgt caccgacctg agggacgtct cccccatcta
      181 cacgggcaac cgcttcctca tctacagtct ctacccggag cagaacgtct ccgtctggat
      241 cgtcgacggc aggcagaagc agaacgtctc catcgcctgc ggatacagca ttctgaaccg
      301 cacgtgcggg gtggacatcg gcgcgctcat gctgaagcac ggaggcggcg gccacaaaat
      361 ggtcggcacc tgccaggtac cgtacagcga ggcggacgac cgcatccgcg agatcgtgga
      421 cgcgctcaag ggatgagacg aatcaggggg agccccgcac gggagctccc cctgtactct
      481 tcccggcgct gtccgcgctc tgctccgccc cgtctaccgc gcttccggcg cggggtgctc
      541 ccccggcccg aagagcatgt actccgaagc gtcgtccgcg gagatccgca tcgcgccctc
      601 catcgggtag tttcccttca ggatgtacgg cacaaggcga aggtagatcg ggtgcatgtc
      661 ctccatcgtg aggctctcgt cgatatccat ccattcgacg gtcccctcct cgctgtcgcg
      721 gggctcccca tcgaagtcgc gcgagatgaa gtagaccgag aggtactcca tatcccgccc
      781 ctcgacgatc gcgtggagca tcccggcgta ctccatgctc cgcaccctgc accccgtctc
      841 ttcctctatt tcgcgcacca gcgcctcgcg cagagtctca tcccgctgct tccgcccccc
      901 ggggacggtg cgctgcccct tgtacgggtc gttggcccgg cggacgatga gcaccttgtt
      961 cccccgcacc aggtatccga agacaatcag cgcggtatac gcgcacccct tccacgattc
     1021 catgccgcac cctctttcca tgtattcccg gcgtcgccgg cgactgctgt tatcatacac
     1081 ctcagaggaa gacccggaac ggatgttcga tcagctcgat caacagggcg aggaactccg
     1141 ccgccggcgc gccgtcgatg accctgtggt cgtgcgccag cgagagcgcc gtcatcggcc
     1201 gcaccgcgac ctctccgtcc attgcgacgg gcatgtcgtt cgtcctgccc accccgagga
     1261 tggccgattc cggctggttg atgacgggcg tgaagaactc cgtggagcgg taccctccaa
     1321 ggttggtcag cgtgaaggtg cctccggaga tctcctccgg agcgagcgcg ttctccctcg
     1381 ccctcctcga caggtccttc agctcgcggc tgatgcgggt caggctcttc tcgtcggcat
     1441 tccggatcac cggaacgatc agcgatcctt cgagcgcaac ggcgacgccc agatgcacct
     1501 cgcggaacgt aacgatgcgg tccccgcgga gcgagacgtt c
//
LOCUS       DAOSWW010000747         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_72516_length_1539_cov_2.251348, whole genome shotgun sequence.
ACCESSION   DAOSWW010000747 DAOSWW010000000
VERSION     DAOSWW010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72516_length_1539_cov_2.251348"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PK442_12650"
     CDS             <1..206
                     /locus_tag="PK442_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88545.1"
                     /translation="SVALNPIIKFTTLFGILAVEIAVESTGVAQWVGLGLLVVGLFFV
                     WRSFYCMRIGSAAKRDAAAETEA"
     gene            complement(368..>1539)
                     /locus_tag="PK442_12655"
     CDS             complement(368..>1539)
                     /locus_tag="PK442_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88546.1"
                     /translation="IGGKAVPLGRDFGRCFSKARLDSADVRADESVPIREERIAQVVQ
                     TLAGARGVFTERHFSRHQAEPHERGEVYGGFFWSLHNRIKALLAESRSAESPAYIAIS
                     LMWEHALHYRVAHPTTEQFVDAMLAGANALFNAETIALDPFDLRDAILDEALVRELVD
                     KKYVAARKKKEDTGIPETLMLQRNGEPAFRSEKDAKERLAGQGRISFLKPTITYTASG
                     VHEFHQQIYTTSDGKAAEVIGNGFVVERVRPKGVRFKRGGEFRHAFTDGVRNLSGSSS
                     IDEKTSYREEAAFKRFAWMVSSMAKKKAASLAALKKKRIKSEKERLGLGMLARDRAAL
                     KYLRESISEGSRFDAGLVILPGAKNLSWRFNAGEIVGYVDAKSGKVTLVRNFIID"
BASE COUNT          270 a          454 c          454 g          361 t
ORIGIN      
        1 cgtcggtggc cttgaacccg ataataaagt tcaccactct cttcggcatc cttgcggtcg
       61 agattgcggt tgaatcgacc ggagtagccc agtgggtcgg gctggggctt ttagtcgtcg
      121 gcctcttctt cgtctggcgc tccttctact gcatgcgcat aggctccgca gcgaagaggg
      181 atgcggccgc agagactgaa gcctgaggat tacggcggtg tcattcagag cggggcgaag
      241 aataccgttc cgcgcggtgt cattctgagt gaaacgaaga atctcgttct gtacaccgag
      301 gataaaatgg gcggctccgg aatcggggcc gcccttactt taggcgcttg ggggaaaacc
      361 gccgccgtca gtcgattatg aagttcctaa cgagcgtaac cttgccgctc ttcgcgtcga
      421 cgtagcccac gatctctccg gcattgaagc gccaggagag gttcttcgcc ccggggagga
      481 tgacgaggcc cgcgtcgaat ctgctgcctt cagagatgga ctcgcgaagg tatttgagag
      541 ccgctctgtc gcgcgcgagc atgccaagcc ccagcctttc cttttccgac ttgatccttt
      601 ttttcttcag cgcggcgagc gaggccgcct tcttcttagc catggatgat accatccatg
      661 cgaaacgctt gaacgccgct tcctcacggt aactcgtctt ttcgtctatg gaagacgagc
      721 cggagaggtt cctgacgccg tcggtgaatg cgtgcctgaa ttcgcctccc cgcttgaagc
      781 gaacgccctt tggcctcacc ctctccacga cgaaaccgtt tcctatgacc tccgcggctt
      841 tgccatcgga ggtcgtgtaa atctgctggt gaaactcgtg gacgcccgac gcggtgtacg
      901 tgatcgtggg cttgaggaag gatatcctgc cctgacccgc gagcctctcc tttgcgtcct
      961 tttcggatct gaaggcgggc tcgccgtttc gctgcagcat cagggtttcg ggtatgccgg
     1021 tatcctcctt tttctttctc gcggcgacgt atttcttgtc cacgagctcg cgcacgagtg
     1081 cctcgtcgag gatggcgtcg cgaagatcga aaggatccaa ggcgatcgtc tcggcgttga
     1141 agagcgcgtt tgcgccggcg agcatcgcgt cgacgaactg ttccgtcgta gggtgcgcga
     1201 ccctgtaatg cagcgcgtgc tcccacatta gtgatatcgc tatgtatgcg gggctttccg
     1261 ctgacctgga ttcggccagc aacgccttga tcctgttgtg gagagaccag aagaagccgc
     1321 cgtatacctc tccgcgctcg tgcggctcgg cctggtgacg cgagaagtgc ctttcggtga
     1381 agacccctct cgctcccgca agggtctgta ctacctgagc gatccgttct tctcttattg
     1441 gtaccgattc gtcagcccgt acatcggcgc tatcgaggcg ggccttggag aagcagcggc
     1501 caaaatcgcg acccaagggg acggcttttc cacctatgt
//
LOCUS       DAOSWW010000748         1535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_72802_length_1535_cov_3.530405, whole genome shotgun sequence.
ACCESSION   DAOSWW010000748 DAOSWW010000000
VERSION     DAOSWW010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72802_length_1535_cov_3.530405"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PK442_12660"
     CDS             <1..347
                     /locus_tag="PK442_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88547.1"
                     /translation="AIAGIAGMCELSGLQGRLQHGFSGGYGYTAIIVAWLARLHPIAI
                     ILVSFLMGVLLVGGETLQIVMRLPLSSMLVLQGLILFFVLGGEFFRKFRIRILSGGAT
                     PPIATTSAKEEK"
     gene            349..1278
                     /locus_tag="PK442_12665"
     CDS             349..1278
                     /locus_tag="PK442_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO88548.1"
                     /translation="MEILIPILTAAVRSGTPILYATLGEIVTEKGGILNLGLEGIMLL
                     GAYAGFAVSYSTGNAWLGIAAAFFVGTLLGLVHAFICVTLKGNQVVSGLAMTMFGTGA
                     SSLLGRNFVGFTIPGLNKIPVPLLSDIPVIGPVLFNHDALIYCSYALVFFLCWFFNST
                     RQGLYLRATGDNPRAADAMGISVAKTRYFYTMVGAGIVAVGGAYMSVAYTKMWAEHMT
                     AGRGWIAVALVIFAIWNPARAAFGAYLFGGVEAFQLRLQAAGTTIPAPILMMLPYLMT
                     IGVLLFISIKKGKGIVFGAPASLGLPFFREERD"
BASE COUNT          264 a          477 c          418 g          376 t
ORIGIN      
        1 gagctatcgc cggaatagcg gggatgtgcg aactctccgg gctgcagggc cgcttacagc
       61 acggtttttc cggcggctac ggctacaccg cgattatcgt ggcatggctg gcccgcctgc
      121 acccgatcgc aatcatactg gtctccttcc tcatgggggt gctcctggta ggcggcgaga
      181 ctcttcagat cgtcatgcgg cttccccttt caagcatgct cgtgctgcaa ggtttgatcc
      241 tcttctttgt tctcggcggt gagtttttca ggaaattccg tatccgcatt ctctccggag
      301 gcgcgactcc tccgatcgca acgacctctg caaaggagga gaagtagcat ggaaattctg
      361 attcccattc tcacagcggc tgtccgaagc ggcacgccta ttctgtacgc gactctcggc
      421 gagatcgtca cggaaaaagg aggcatcctc aacctcggac ttgaaggcat catgctcctg
      481 ggcgcgtatg cgggattcgc agtctcctac tccacgggga acgcatggct tgggatcgct
      541 gcggcattct tcgttggaac gctcctgggg cttgttcacg ctttcatctg cgtcacgctg
      601 aaaggcaacc aggtagtcag cggtctcgcc atgaccatgt tcggtacggg cgcgtcctct
      661 ctcctgggcc gcaatttcgt cggcttcacc atcccggggt tgaacaagat tcccgttccg
      721 ttgctctccg acatccctgt catcggacct gtgctcttca atcacgatgc gctcatctac
      781 tgctcgtacg ccctcgtctt ctttctctgc tggttcttta actccacaag gcaggggctg
      841 tatctccgcg ccaccggaga caaccctcga gccgccgacg cgatgggcat ctccgtcgcg
      901 aagacgcgct acttctacac gatggttgga gcgggtatcg tagctgtggg cggcgcgtac
      961 atgtccgtgg cctatacgaa gatgtgggcg gagcacatga ccgccggacg cggatggatt
     1021 gcagtcgcgc tcgtcatctt cgccatctgg aaccctgcgc gcgcagcctt cggcgcctat
     1081 cttttcggcg gtgtggaagc cttccagctc agacttcagg ctgcgggaac cactatccct
     1141 gcgccaatcc tgatgatgct cccctacctg atgacgatcg gcgtccttct cttcatctcc
     1201 attaagaaag ggaaagggat cgtcttcgga gcgcctgcct ccctgggact tccttttttc
     1261 cgggaggaac gcgactaact ggtttcccga acgcacacac gctcatcggg cgtcggcgat
     1321 cgatacggcg cccgatgacg acggccgccg gatatactct ttcaccatac aggaggttgt
     1381 tccaaagtgg attttttctt tcagcacatc cccgtttcgg ggagcattct caatgcggcg
     1441 gcaatcgctc tcggcggcct cgccggtctt ttgctccatt cccgtctttc gcaacggttt
     1501 atcaccatta cttttcaggg actgggactc ttcac
//
LOCUS       DAOSWW010000749         1533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_72974_length_1533_cov_2.401218, whole genome shotgun sequence.
ACCESSION   DAOSWW010000749 DAOSWW010000000
VERSION     DAOSWW010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72974_length_1533_cov_2.401218"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PK442_12670"
     CDS             complement(<1..778)
                     /locus_tag="PK442_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO88549.1"
                     /translation="MHPHLFTPGPVEVPHSVRAAGAAPLVGHRSEAYFALLEEIAALL
                     KKLLRASQPVITLPSSGTGALEALAANLLDPGDAVISFSCGAFGDRFREIASRRGATI
                     LPVDIPRGRTATPSDVRLALEQHSGAAAMLFTHNETSTGALTDLRACTSALPEDGPLV
                     LVDAVSSFGAVPCFPEEQRIDGLASCSQKGLLTPPGIGIVALSERGWKRAASRPSPSY
                     YFDLPLHRRFLEKARPQNPYTPPVTLLYSLAEALRFLDGVD"
     gene            777..1070
                     /locus_tag="PK442_12675"
     CDS             777..1070
                     /locus_tag="PK442_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88550.1"
                     /translation="MDDPSFEYAKRPEGRVPSGLYSLRNSDDSIPRVRNCYGCGVSPA
                     GTGRPALPDQPEEEEELLPMPLKRTVRAMKIRSLKKDVHVISVKAINVNRFLR"
     gene            1204..>1533
                     /gene="dctP"
                     /locus_tag="PK442_12680"
     CDS             1204..>1533
                     /gene="dctP"
                     /locus_tag="PK442_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO88551.1"
                     /translation="MKRMCGVAALAVLLAVVLTGAAFAAFAAPEYVIKVGYILPENQS
                     DHIIMRDVFKKDIEEKSGGRISVELYPNAQLGGDRELIESVQLGTVQVAIPATSALAG
                     FDKRFQVF"
BASE COUNT          313 a          445 c          524 g          251 t
ORIGIN      
        1 agtcgacccc gtcgaggaag cgcagcgcct ccgcgaggct gtagagcagc gtgacgggcg
       61 gcgtgtaggg gttctgcgga cgcgccttct ccagaaaacg gcggtgcagc ggaaggtcga
      121 agtagtagga gggcgacgga cgcgacgccg cgcgcttcca tcctcgctcg gagagggcga
      181 cgattccgat tcccggaggg gtcagcaacc ccttctgcga gcaggaggcg aggccgtcga
      241 tgcgctgctc ctccgggaag caggggacgg ccccgaacga gctgaccgca tcgacgagga
      301 cgagcggccc gtcctccggc agggccgagg tgcaggcgcg aagatcggtg agcgcacccg
      361 tcgaggtctc gttatgtgtg aagagcatcg ccgccgcgcc ggagtgctgt tcgagggcca
      421 gccgcacgtc cgaaggcgtc gccgtccgtc cgcgggggat gtcgacgggg agaatagtcg
      481 cgcccctccg cgacgcgatc tcgcggaagc ggtcgccgaa cgcgccgcac gagaaggaga
      541 tgacggcgtc gccggggtcg agcaggttgg cggcaagggc ctccagcgcg ccggtgcccg
      601 acgacggcag cgtaatcacg ggctgcgagg cgcgcagcag cttcttcaag agcgcggcga
      661 tctcctccag cagcgcgaag tacgcttcgc ttctgtgccc gacgagaggc gcggcgcccg
      721 ccgcgcggac ggaatgcgga acctcgaccg gtcccggcgt gaaaagatgg gggtgcatgg
      781 acgatccctc ctttgaatat gcgaaaaggc cggaagggcg tgtcccctcc ggcctctatt
      841 ccctgcgaaa ttccgatgat tcaattccaa gggtgcgaaa ctgctacggc tgcggcgtct
      901 cccctgccgg cacaggaaga ccagcccttc cggaccaacc ggaagaggag gaggaactac
      961 tacccatgcc gttgaaaagg actgtacgcg ccatgaaaat ccgctccctg aaaaaagatg
     1021 ttcacgttat atcagtcaaa gctatcaatg tcaataggtt tttgcgataa tacgtaccgg
     1081 tggcgccagc ccgaaaaaaa gactatacct tgctactgcg ctgtagtatt atagcaatcg
     1141 cagaaaatct tccggatacg gaaggccgga gtatgagccg gtatatcaaa aggaggggtt
     1201 gtaatgaagc gtatgtgcgg agtagcggca ttggcggtac tgctggcggt cgtgctgacg
     1261 ggcgcggcgt tcgcggcgtt cgcggcgccc gagtatgtga tcaaggtcgg gtatattctt
     1321 ccggagaacc agtcggacca catcatcatg cgggatgtgt tcaagaagga tatcgaggag
     1381 aagagcggcg gcaggatctc cgtcgaactc taccccaacg cgcagctcgg cggcgacaga
     1441 gagctcatcg aatccgtcca gctggggacg gtgcaggttg ccatcccggc gacgtcggcg
     1501 ctcgccggtt tcgacaagcg cttccaggtg ttc
//
LOCUS       DAOSWW010000750         1530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_73178_length_1530_cov_3.002712, whole genome shotgun sequence.
ACCESSION   DAOSWW010000750 DAOSWW010000000
VERSION     DAOSWW010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73178_length_1530_cov_3.002712"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..>1530
                     /locus_tag="PK442_12685"
     CDS             79..>1530
                     /locus_tag="PK442_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88552.1"
                     /translation="MRTLSAETSYSRRLDALFKHPAADAAMLVLISMSVAVAAGSLLV
                     DNEELEHLNLYFSLLFSVELTARYFTYASNKREYFADWWMDWIAAIPWDIFLFFLFPG
                     GGASMLRLLRLPRIFRLLRFRHLKHSDAARRLSYRFRRLMEVSIFRQILTMLLVSFVF
                     VWIFTTVLDGLGVQSEHGNNFWFSIITMISSDSIFEIHNQETLVKVLVLIFSFIGIVL
                     FNGVLIAIIIGKLMEHLDDLKQGRGDVRERGHIILLGWNECIPHIVDELESYCRTERK
                     RLIRVVVMREHPPESPDQVVESRPHVEVISRTGSFQNAQALERISAHKASAVVVLGGR
                     AADRKLSERLNDPIVTRTLVALETLLDSKPNERHSPVIVLNYLDLSRSCHVTEFLRPF
                     GNRSTKVFFNPLFFTGKLIASMCINPYAEDIFNELLTPEGNEFHFVRLPPGEKMLWRD
                     MMNAFPKSIPVGYRDASGELRMVRQECMATVGAV"
BASE COUNT          294 a          480 c          436 g          320 t
ORIGIN      
        1 ttcgcgctcg taaaaggccg cctgcttcag tagattctac cggtgtatgc tccggcggta
       61 tgagaaggag gagatgtcat gcggactctt tccgcggaaa caagctattc acggcggctg
      121 gacgcgctct tcaagcaccc cgcggcggac gcggcgatgc tcgttcttat atcgatgtcc
      181 gtcgcggtcg cggcaggatc gctgcttgtc gacaacgaag agctcgaaca cctcaacctc
      241 tatttctcgc tcctcttctc ggtggaactg acggcgcgct acttcaccta cgcgtcgaac
      301 aagcgagagt acttcgccga ctggtggatg gactggatcg ccgccattcc gtgggatatt
      361 ttcctcttct tcctcttccc ggggggaggc gcctcgatgc tccgattgct ccggctcccg
      421 aggatcttcc ggctgctccg attccgtcac ctcaagcact cggacgccgc gcgccggctc
      481 tcctaccgct tccggcgcct gatggaggtc tccatcttcc ggcagattct gacgatgctt
      541 ctcgtttcgt tcgtcttcgt ctggatattt acaaccgttc tggacggatt gggcgtgcag
      601 tccgaacacg gaaacaactt ctggttctcc atcatcacga tgatcagctc ggacagcatc
      661 ttcgagattc acaatcagga gacgctggtc aaggtgctcg tccttatttt ctcgttcatc
      721 gggatcgtcc tcttcaacgg cgtcctcatc gccatcatca tcggcaagct catggaacac
      781 ctcgacgacc tgaagcaggg gcgcggcgac gtgcgcgaac gggggcacat catcctgctc
      841 ggctggaacg aatgcatccc gcacatcgtc gacgagctgg agtcctactg cagaacggaa
      901 cggaagcgcc tgatcagagt ggtggtgatg cgggaacacc cgccggaatc acccgatcag
      961 gtcgtcgagt cgaggccgca cgtcgaggtg atctcgcgta cgggcagctt ccagaacgca
     1021 caggcgctcg aacgcatctc cgcgcacaag gcgtcggcgg tagtcgtcct cggaggacgg
     1081 gccgccgaca ggaagctctc ggagcggctg aacgacccca tcgtgacccg gacgctcgtc
     1141 gctttggaaa cgctgctcga cagcaagccg aacgaacggc attctccggt gattgtgctg
     1201 aactacctcg acctcagcag aagctgccat gtgaccgagt tcctgcgccc gttcggcaac
     1261 aggagcacaa aagtcttctt caacccgctc ttcttcaccg gcaagctgat cgcctccatg
     1321 tgcatcaacc cctacgcgga ggacatcttc aacgaactgc tcacacccga ggggaacgag
     1381 tttcacttcg tccgcctgcc cccgggagag aagatgctct ggcgcgacat gatgaacgcc
     1441 tttccgaaat cgattcccgt ggggtaccgg gatgcatcgg gcgaactgcg gatggtccgc
     1501 caggaatgca tggcaacggt tggcgcggtt
//
LOCUS       DAOSWW010000751         1528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_73347_length_1528_cov_2.666667, whole genome shotgun sequence.
ACCESSION   DAOSWW010000751 DAOSWW010000000
VERSION     DAOSWW010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73347_length_1528_cov_2.666667"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..973)
                     /gene="ftcD"
                     /locus_tag="PK442_12690"
     CDS             complement(59..973)
                     /gene="ftcD"
                     /locus_tag="PK442_12690"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOO88553.1"
                     /translation="MAGKLIECVPNFSEGRRQDVIEAIVAPFRATKGCGLLDYRADAD
                     HNRLVVSLAGSPEPVQEALLKAAAVAVQKIDMNSHQGAHPRIGAVDVIPFTPVSNISM
                     EECVALSHSFGERYWKELGVPVYFYEDSAKRPERKRLEVIRKGQYETLKTESVTPERA
                     PDVGGPALHPTAGATVMGARKFLVAFNVNLNTADEEIAKKIANCVRASSGGFCHVKGM
                     GVALHERGMAQVSMNIVDFEKNAVYRVLELIRMEARRWGVEVVETEIYGMIPAQALVE
                     SAAYYLQIKDFDPMQVLELKLLSGGDTE"
     gene            complement(1106..>1528)
                     /locus_tag="PK442_12695"
     CDS             complement(1106..>1528)
                     /locus_tag="PK442_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO88554.1"
                     /translation="LESFCNLKQAADLHTSTISQYEIDAYLSDYDIEEHIKKLVSVYR
                     ERRDLMIRTMDSAFPKEVVFTRPEGGLFTWAEMPEHVNARDLFLKALEKKVAFVPGGA
                     FFPNGGHENTMRLNYSNMPPERIVEGIERLGAAIREYL"
BASE COUNT          294 a          471 c          430 g          333 t
ORIGIN      
        1 cttgaggggg gttttccctc cggagtgtag atgcgggcgt tgcggaaaaa tttgacgatc
       61 attcagtatc accgccggag aggagcttca gctccagaac ctgcatcggg tcgaagtcct
      121 tgatctgcaa atagtacgct gcgctctcca caagagcctg cgcaggaatc attccgtaga
      181 tctccgtctc gacgacctcg acgccccatc gtctggcctc catccggatc agttcgagca
      241 ccctgtacac cgcgttcttc tcgaagtcga cgatattcat gctcacctgc gccatgccgc
      301 gctcgtggag cgccacgccc atgcccttga catggcagaa gccgccgctg gaggcgcgga
      361 cgcagttggc gatcttcttc gcgatctcct cgtccgcggt gttcaggttg acgttgaagg
      421 ccacgaggaa cttccgggcg cccatcaccg tcgcgcccgc cgtcgggtgc agggcgggac
      481 cgccgacgtc gggagcgcgc tccggtgtga cggactcggt cttgagcgtc tcgtactgcc
      541 ccttgcggat aacttcaagg cgcttccgtt ccgggcgttt cgcggaatcc tcgtagaagt
      601 agaccggaac gccgagttct ttccaatatc tctcgccgaa cgaatgcgag agcgccacgc
      661 actcctccat agaaatgttg ctcacggggg tgaaaggaat cacgtccacc gcgccgatgc
      721 gcggatgcgc cccctggtgg ctgttcatgt caatcttctg caccgcgacg gcggcggcct
      781 tgagaagcgc ctcctgaaca ggctcgggcg atcccgcgag gctcacgacc agcctgttat
      841 ggtccgcgtc cgcccgataa tcgagaagac cgcacccctt cgtcgccctg aacggagcga
      901 cgatcgcctc gatcacatcc tgacgccgtc cttcgctgaa attcggaaca cactcgatga
      961 gcttgcctgc catgggaaca acctcctgca ggtattagta tgctgcacgg tacatgataa
     1021 cattgaaaca tgtacaacgt aaagcgcgaa agtaaaaaga gaggcaggac cccttgtttc
     1081 agccctgcct ctctcattcg tttttttaga gatactcgcg gatcgccgct cccaggcgtt
     1141 cgatcccttc gacgatgcgc tccggcggca tgttggagta gttgagccgc attgtgttct
     1201 cgtgcccccc gttggggaag aacgctcctc cggggacgaa ggcaaccttc ttctcaagcg
     1261 ccttgaggaa aaggtctcgc gcgttcacat gctccggcat ttctgcccat gtgaagagcc
     1321 ctccctccgg gcgggtaaaa acgacctcct tcgggaatgc agagtccatc gtgcgtatca
     1381 tcagatcacg ccgttcacgg tatacggaga ccagtttctt gatgtgttcc tctatatcgt
     1441 agtcggagag ataggcgtcg atctcgtact ggctgatcgt cgacgtatgg aggtctgcgg
     1501 cctgtttcag attgcagaag ctctccag
//
LOCUS       DAOSWW010000752         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_73399_length_1527_cov_4.023777, whole genome shotgun sequence.
ACCESSION   DAOSWW010000752 DAOSWW010000000
VERSION     DAOSWW010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73399_length_1527_cov_4.023777"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="dctP"
                     /locus_tag="PK442_12700"
     CDS             <1..871
                     /gene="dctP"
                     /locus_tag="PK442_12700"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO88555.1"
                     /translation="EASGGRLEIKPFVGGSIVPAYKELDAVHENVLQLCYTCPMYNLD
                     KWSAAGLISSRPGALAGEALRTWFNYGGGADLMNKMMEGYNVMTFPGALSPLPEEVFF
                     HSKKKIESLEDLKAIKARCMGDGGEILKRMGMATVIIPGGELYEAMKRGTIDAFEYST
                     LASNWNMHFNEVADYVYLSPVRAPSDPQVFFVNKDAWNALPKDLQLLVQTFVDRFSQA
                     QHEFLVYESIIALEKFKEAGNQVLKVPEEVNVALAAEAAKFYEEKIASEPPIFGEIYN
                     SMKKFGEAYQTMK"
     gene            906..1424
                     /locus_tag="PK442_12705"
     CDS             906..1424
                     /locus_tag="PK442_12705"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOO88556.1"
                     /translation="MTSLKRFLKKIDALSELSGSVGKWCALLLVFAGTYEAVSRHFFN
                     APTVWAYDTLCMAGGALYLLGASYDYLHEAHTRVDIFYNMLPERGKALMNVVCALFLF
                     FPLMGVMLWLSVTWAVRAWTINEVFFNSFWYPPAGPYRTIFAVGLVLLLLQAVAKFVR
                     DFYFMVRGETLD"
BASE COUNT          313 a          437 c          421 g          356 t
ORIGIN      
        1 cgaggcttcg ggcgggcgcc tggagatcaa gccgttcgtc ggcggctcca tcgtccccgc
       61 ctacaaagag ctcgacgccg tccacgagaa cgtgcttcag ctgtgctaca cctgcccgat
      121 gtataatctc gacaagtggt ccgcggcggg cctgatcagc tcccgtcccg gcgcactggc
      181 cggagaggct ctccggacgt ggttcaacta cggcggcggc gctgatctga tgaacaaaat
      241 gatggaaggg tacaacgtca tgaccttccc cggcgcgctc tctcctcttc ctgaagaggt
      301 tttcttccac tccaagaaga aaatagagag tcttgaggat ctgaaggcga tcaaggcccg
      361 ttgcatgggc gacggcggcg agatcctgaa gagaatgggc atggcgacgg tcatcatccc
      421 cggcggcgag ctctacgaag caatgaagcg cggtaccatc gacgctttcg agtactccac
      481 gcttgcctcc aactggaaca tgcacttcaa cgaagttgcc gactacgtct acctctcccc
      541 ggtacgtgct cccagcgatc cccaggtctt cttcgtgaac aaggatgcct ggaacgcgct
      601 tccgaaggat ctgcagctcc tcgtccaaac gtttgtcgat cgcttctctc aggcgcagca
      661 cgaattcctc gtctacgagt ccatcatcgc ccttgaaaag ttcaaggaag ccggcaacca
      721 ggtgctgaag gttcctgaag aagtaaatgt ggcgcttgca gccgaggccg cgaagttcta
      781 cgaagagaag atagccagcg aacctccgat tttcggcgag atctacaact ccatgaagaa
      841 gttcggcgaa gcctaccaga caatgaaata acgcgtcttc gacgacgcgc acaaggagaa
      901 gcggtatgac ctctttgaaa cggtttttga aaaaaatcga tgcacttagc gaactgtccg
      961 gatccgtggg gaaatggtgc gcccttctcc ttgtgttcgc cgggacgtat gaagctgtct
     1021 caagacactt cttcaacgcc cccacggtct gggcgtacga tacgctctgt atggcaggag
     1081 gcgccctcta tcttctgggc gcttcctatg actacctcca cgaggcgcac acgcgcgtgg
     1141 acatttttta caacatgctc cccgaacggg ggaaggcgtt aatgaacgtc gtctgtgcgc
     1201 tcttcctttt cttccctctg atgggggtca tgctctggct ctccgttacg tgggcagtgc
     1261 gcgcctggac gatcaacgaa gtcttcttca acagcttctg gtaccccccg gcggggccgt
     1321 accggaccat tttcgcagtc ggtctcgtct tgctgctgct tcaggctgta gcgaagttcg
     1381 tcagggattt ttactttatg gtaaggggtg agactcttga ttgaactcag ccctgagctt
     1441 gttactatct taatgctggg tggagtcctg ttccttgtca tgacggggtt tcccatcgct
     1501 tttgtgatcg ggagcgtagc tttcttt
//
LOCUS       DAOSWW010000753         1525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_73567_length_1525_cov_2.740816, whole genome shotgun sequence.
ACCESSION   DAOSWW010000753 DAOSWW010000000
VERSION     DAOSWW010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73567_length_1525_cov_2.740816"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1445)
                     /locus_tag="PK442_12710"
     CDS             complement(156..1445)
                     /locus_tag="PK442_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88557.1"
                     /translation="MRKRVELRVKDAHSVPEREQLLAKILPETPKEVLEHFLIETASR
                     SSERYVELLEYISAGTSRPSVVERLAAEEKILSLWRSAEPSLSDLYEYGGLYEDEEEE
                     MYDLLGDLSNALNEGLAGRECRREITDNVFEYLEKDDYGTHELLYDIALSACTDEDDL
                     DYLSRTLEEQGDSLEERYGPASMMKYYKKSGDREKYLAIRLENLHRAEDYLDLAEFHR
                     DSGDWEEAMKVAEAGLEKADGNKYTLRSFVIDRLDATGKRARATELRFDQFFSAAFFK
                     EYLKFRRECSDEEWAAYEERVAERMKSAYPDARLFYHMHRGESSDALTILLQHGRKLY
                     WNSDVFAAAEKLEADHPERMYDCYVRSMGNLYETAGRDVYALKARLMAAVRRVLVERL
                     GEDAKWTAFAREVKKGNSRRAALQDEFAKVIPGWRKL"
BASE COUNT          327 a          471 c          360 g          367 t
ORIGIN      
        1 gcattcacca taccgctcac cccccgaacg catttgtatt gctttttgcg ttacatctct
       61 agagggattc ccgataccgg cgtccagaca ctctactttt tctgatgatc gaatgagaag
      121 gggctcggag aaagagcaca aaaacaagaa agcaactaca gcttcctcca tccgggaatg
      181 actttggcga actcgtcctg aagggcggcc cgcctggaat tgcctttttt cacctcgcgg
      241 gcgaacgccg tccacttcgc atcctcgccc aaacgctcga caaggacgcg ccggactgcc
      301 gccatcagcc gagctttgag cgcgtacaca tctcttccgg cggtctcgta gaggttcccc
      361 atgctccgga cgtagcagtc gtacattctt tcggggtgat cagcctcaag cttttccgca
      421 gctgcaaaaa catcgctgtt ccagtagagc tttctcccgt gctgcaaaag gatcgtcagc
      481 gcatcggagg actctccgcg atgcatgtga tagaagaggc gagcatccgg atacgcgctt
      541 ttcatgcgct ccgcgaccct ctcttcgtag gcggcccact cttcgtcgga acactcccgg
      601 cgaaacttca ggtactcctt gaaaaatgcc gcgctgaaga actgatcgaa acgcagttcc
      661 gtcgcccgcg ctctctttcc cgtcgcgtcc agcctgtcta tgacgaagga gcgaagcgtg
      721 tatttattgc cgtcggcctt ttcaagcccc gcctcggcca ctttcatggc ctcctcccaa
      781 tctcccgagt ctctatggaa ctccgcgaga tcgagatagt cctcggcccg atgcaaattc
      841 tcaagccgaa tcgcgagata cttctcccgg tcgccgcttt ttttgtagta cttcatcatg
      901 gaagcaggac cgtatctttc ttccagagaa tccccctgct cctcgagcgt ccggctcaga
      961 tagtcgagat cgtcctcgtc cgtacacgcc gaaagggcga tgtcgtacag aagttcgtgg
     1021 gttccgtagt catccttctc cagatattcg aacacattgt ccgtaatttc cctccggcat
     1081 tctctgcccg caagcccttc gttcaaagcg tttgaaagat ccccaaggag atcgtacatc
     1141 tcctcctcct cgtcctcata caaacctccg tactcgtaca gatccgagag gctcggttcc
     1201 gcgctccgcc aaagagagag aatcttctcc tccgcggcga gtcgttcgac tacggatggc
     1261 cgggacgtac cggcggagat gtattcaagc agttcaacat atcgctccga agatcgggac
     1321 gcggtctcta tcagaaaatg ctccagcact tctttcggcg tctccggaag aatcttcgca
     1381 aggagctgtt cccgttccgg cacactgtgc gcatctttaa cgcgaagttc gactcttttt
     1441 ctcatccttg tagagatctc ctctcttgaa cagtccaaaa aacagaagag attgccgaag
     1501 cctggaaaga ggttttatat aatgt
//
LOCUS       DAOSWW010000754         1523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_73730_length_1523_cov_2.376022, whole genome shotgun sequence.
ACCESSION   DAOSWW010000754 DAOSWW010000000
VERSION     DAOSWW010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73730_length_1523_cov_2.376022"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1150
                     /locus_tag="PK442_12715"
     CDS             257..1150
                     /locus_tag="PK442_12715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOO88558.1"
                     /translation="MARVIFLPVEVGISRKNGTIQLTEKQGGRKAKVPVLQTSEIFAL
                     SGAEPDGTLLSLLAQYTVPLHIFKDGTHISTYIPFASVLSGKSVLAQVRTLEDTQQRW
                     SLAREILRGGARLRCMASRMLRPEEKEEWECRYMDCLAAGYAAGTNGTAESLRAFDRI
                     DRDFLCREQWRDEELRYAEGLSKGVVLSALPRLSLDPWIGILQQEKTDIPPLAADMLF
                     LFSPLLTWLWPRKKRPSPARRDLPETFRRHMGRRGATNGARIWSLKYLPVREGYALSA
                     RFSTGRPYRCASRVEVERCAE"
     gene            1140..>1523
                     /locus_tag="PK442_12720"
     CDS             1140..>1523
                     /locus_tag="PK442_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88559.1"
                     /translation="MQNEQTIADGIALRCSLASMYYLDGIHSSEEALEDRSLFWERPF
                     LPSLDSQDEDEAEKAVANIYEEMERREGLALLTANGLPPYASVGAMLFMLYDNHLEKG
                     WITRKDRDPLEVRLTEKGRKLLEKAE"
BASE COUNT          384 a          416 c          414 g          309 t
ORIGIN      
        1 aaaacatgat acccgcaaag ttcggacgtc tccgcggacg attgactttc acacagcacc
       61 aaatacgact gcattggcgt tccctccttc tttttgaaga atactatatc aagaatgcac
      121 cgtgaataca gcatttcatc cacctttgtt tgcggaaaaa gacgcagact gccaatgttc
      181 ggtcgattac ggaaatgaag gaacttcttt cgaggagaac ttcggatagt ctcatacaaa
      241 acgaaggagg tcgcgcatgg caagagtcat atttcttcca gtcgaagttg gcatatccag
      301 aaagaacgga accattcagt tgactgaaaa acagggcgga aggaaggcga aagttcctgt
      361 gttgcagacg tccgaaatct ttgcgctctc cggagccgaa ccggacggga cgctgctttc
      421 gctgctcgct cagtacaccg ttccgttgca tatattcaag gatggaacac acatctccac
      481 ctatatcccg ttcgcctccg tactttcggg aaagagcgtg ctcgctcagg tgcgtacgct
      541 tgaagacact caacagcgct ggagtctggc gcgggaaatc cttcgagggg gcgcgcgtct
      601 gcgctgcatg gcctccagaa tgcttcggcc ggaagaaaaa gaagagtggg aatgcagata
      661 catggactgc ctcgctgcag gctatgcggc cggaacgaac ggaacagcgg agtccctccg
      721 cgcattcgac cggatcgacc gcgacttcct atgccgcgaa cagtggcgcg acgaggaact
      781 gcgatacgcc gagggattga gcaaaggggt cgtactgagc gccctccccc gtctttcgct
      841 tgacccgtgg atcggaattc tgcaacagga aaaaacggac attcctccct tagccgctga
      901 catgctcttt ctcttctcgc cgctgctcac atggctttgg ccgcgcaaaa aacgcccttc
      961 ccccgcccga cgcgatctgc ccgaaacgtt ccggcggcac atgggtaggc gaggtgcgac
     1021 aaacggagcc agaatatgga gcctcaagta tcttcccgtg agagaagggt acgcgctttc
     1081 agcccgcttt tcaacgggca ggccgtaccg gtgcgcatcc agagtggagg tggaacgctg
     1141 tgcagaatga acagactatc gccgacggaa tcgcgctgcg ctgttcactg gcctcgatgt
     1201 actatctcga tgggattcac tctagcgagg aagcgcttga agacaggtcg cttttttggg
     1261 agaggccctt cctcccctcc ctggacagcc aagatgaaga cgaagcggaa aaagccgtcg
     1321 caaacatcta cgaagaaatg gagcgaagag aaggtcttgc tttactaaca gcaaacggac
     1381 tgcctccgta cgcttccgtc ggggctatgc tcttcatgct ctacgacaac catctcgaaa
     1441 aaggatggat taccaggaaa gatcgagacc cgcttgaagt acgtcttacc gaaaaaggac
     1501 gaaagctgct cgaaaaggcg gag
//
LOCUS       DAOSWW010000755         1522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_73769_length_1522_cov_3.875937, whole genome shotgun sequence.
ACCESSION   DAOSWW010000755 DAOSWW010000000
VERSION     DAOSWW010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73769_length_1522_cov_3.875937"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PK442_12725"
     CDS             <1..694
                     /locus_tag="PK442_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOO88560.1"
                     /translation="RLQPISMLTGQAAGTIAALSVREGKAARALDPRLVQLHLLEAGS
                     ALSLCEYHDVPRNHLFWPGVQMSNLYGWFTPLDLPSSPSAKIDDIYNNRVVLARLFGL
                     DKGVFGVDAPLTGAEAETLLHKAFGEEKDALLYALPTFGLSSFISRGDFADALARILG
                     YRATPEEFVSRFSDISSTSRLAGPMHFLSEKGVLDRVGASGVFMPDSLLTRGAAADMT
                     MRAVVAPRERRR"
     gene            691..1278
                     /locus_tag="PK442_12730"
     CDS             691..1278
                     /locus_tag="PK442_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HOO88561.1"
                     /translation="MKCHLCETKSCSSGEACADFESLELYQGNEEDLQLLKTASDVEA
                     LYYCTLNRLEEIMEFARRMGYTRLGIAFCVGFKEEAALLGKILSEEFEVYSACCKVSS
                     MKKADVDATLRPWVGEVSCNPVEQARRLNEAGSQFNIVLGLCVGHDSLFYRHSKAPVT
                     TFVVKDRVLGHNPVAVLYSQYLRKNLKKRPEERLK"
BASE COUNT          315 a          402 c          457 g          348 t
ORIGIN      
        1 gcgccttcaa ccaatttcga tgctcactgg acaggctgcg ggaacgattg cggcgctctc
       61 agtcagagag ggaaaagcgg cgagggctct cgacccgagg cttgtgcagc tgcatctgct
      121 ggaggcaggg agcgcgctct ccctctgcga ataccacgac gtcccgcgaa atcatctctt
      181 ttggccgggc gtccagatgt ccaacctcta cggatggttc acgccccttg acctgccttc
      241 gtccccttcg gcgaaaatag acgatatcta caacaaccgg gttgtgctgg cccgtctctt
      301 cggacttgat aaaggggtgt tcggcgtcga cgccccattg acgggggcgg aagcggagac
      361 gctgctccat aaagcgttcg gtgaagagaa ggacgctttg ctgtatgccc ttcctacatt
      421 cggactttcg tcttttatct cgagggggga ttttgccgac gcgctcgcga ggatattagg
      481 gtacagagca actcctgaag agtttgtctc ccgcttcagc gatatttcct ccacctcgcg
      541 tctcgcgggc ccgatgcact tcctctcgga gaagggcgtt ctggaccgcg tcggggcatc
      601 cggcgtcttt atgccggact ctttgctgac gcgcggagct gccgcagaca tgacgatgag
      661 agcagttgtc gcgccgaggg aacgacgaag atgaagtgtc acctctgcga gacgaagagc
      721 tgttcctcgg gtgaggcgtg cgctgatttt gaaagcctgg aactctacca gggaaatgaa
      781 gaagaccttc aacttctcaa gacggcctcc gatgttgaag cgctctacta ctgtaccctc
      841 aaccggctgg aggaaataat ggagtttgcc cggcggatgg ggtatacgcg tctgggcatt
      901 gccttctgtg tcggttttaa agaggaggcg gctcttctgg ggaagatcct ttcggaggaa
      961 ttcgaagtgt actccgcatg ctgcaaggtc tcctcaatga agaaggcgga cgttgatgca
     1021 acgcttcgtc cctgggtcgg cgaagtgtcg tgcaaccccg tcgaacaggc gcgtcgcctc
     1081 aacgaagcgg gaagtcagtt caacatcgtc ctcggcctct gcgtgggaca cgacagcctc
     1141 ttttaccgtc actcgaaggc gcctgtcacg accttcgtcg tcaaggaccg cgtgctcgga
     1201 cacaacccgg tagcggttct ttacagccag tacctcagga aaaatttgaa gaaacgtccc
     1261 gaggagaggt tgaagtagag cggaacaaag ggaggccgag gagaatgcgg cctccctttt
     1321 tcttcggtac ctcatttttt aggtgtgacc attcaggaaa atggcgcgtg cggatggccg
     1381 tgaggggcgt cttgttttat aatgattcag gacgtatgac gtcgtacgta agggttgata
     1441 gtgattgaag agaatgagaa aaagagcggc tgcgctctcg atgcagacag ctctcgcgct
     1501 tgctttcacg ctttttgttc tg
//
LOCUS       DAOSWW010000756         1522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_73797_length_1522_cov_2.773006, whole genome shotgun sequence.
ACCESSION   DAOSWW010000756 DAOSWW010000000
VERSION     DAOSWW010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73797_length_1522_cov_2.773006"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PK442_12735"
     CDS             <1..1077
                     /locus_tag="PK442_12735"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88562.1"
                     /translation="LGGALEQAPQDPGRRGAAAGRPQRRRPVAVEHRLQARREVALEA
                     VLEPAFGVEPGPQLGGDLLWGRMLAASARAPLFCYSYGRKNGIERCAALFTAYREMAE
                     SIASSGEGQSLQRTGRILLAGDLAAELPDTFVHAPDGEDRIPEGRPRVAFFPGSREHI
                     REYAVPLIRELAAALRESFPSLDARIILSPFASAGEEEAFRRSGDLTPVRGGSKESLR
                     GLDFAVTQPGTNTLELMHCAVPFLVAVPFSSLRHIPLPGVAGALANIPLIGPALREAA
                     LKAKGRRTGFVAWPNRLAGRQVVDELIGDISARDVARVVGGHLLDGEYLARTRKSLAA
                     LSSSAPRNAAVFMADHIERMLAER"
     gene            1074..>1522
                     /locus_tag="PK442_12740"
     CDS             1074..>1522
                     /locus_tag="PK442_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOO88563.1"
                     /translation="MTSEEKTASPSPYLRLLAYARPYSRRFFLALGCMVAASACNVVP
                     PWLLKNVVDDVLIAKNLAMLNALPFILVALFAGKGIASYGHQYLMNWVGQNVVMDLRL
                     RLYDHLQRMSLKYLYGKRLGELMSRITNDVTILQNLVTSVLVNLVVQA"
BASE COUNT          243 a          509 c          484 g          286 t
ORIGIN      
        1 ctcggcggcg ccctggaaca ggccccccag gacccaggtc gacgcggcgc ggccgccggc
       61 cggccgcagc ggcgacggcc ggtcgcggtc gagcaccgcc tccaggcgcg gcgcgaggtc
      121 gcgcttgaag cggttctgga accagcgttc ggcgtggagc ccgggccgca gctcggcggc
      181 gacctgctct gggggcggat gctcgccgcg agcgcccggg cgccgctctt ctgttactcg
      241 tacgggcgaa aaaacgggat agaacgctgc gccgccctct ttaccgccta cagagagatg
      301 gcggagtcga tcgcctcctc cggcgagggg caatcgctgc aacggacagg acgaattctc
      361 ctggcgggcg atcttgccgc cgagctgccg gatactttcg tgcacgcgcc cgacggcgaa
      421 gaccgcatac ctgagggacg tccaagagtt gccttcttcc ccggaagccg ggaacacatc
      481 agggagtacg ccgtgccgct gatacgcgag cttgctgcgg cgctgcgaga gtcgttccct
      541 tcgcttgacg cccgcattat cctttctccc ttcgcctcgg ccggggagga ggaagcgttc
      601 cgtcgctccg gagacctgac cccggtgcga ggaggaagca aagagtcgct ccggggcctt
      661 gacttcgctg tgacgcagcc gggaacgaac acgctcgaac tgatgcactg cgccgttccc
      721 tttctcgtcg cggttccgtt ttcttcactt cgccatattc cgctgcccgg cgtcgccgga
      781 gcgctggcca acattccgct gatcggccct gcgctgcgcg aagccgcgct caaggcgaaa
      841 gggcgccgga cggggtttgt ggcctggccg aaccgcctcg cagggcgcca agttgttgat
      901 gaactgatcg gcgatatctc tgcgcgggat gttgcccggg tcgtcggagg gcatcttctg
      961 gacggcgagt acctcgcccg gacgaggaag tcgctcgccg cgctctcttc ctccgcgccc
     1021 cggaacgccg ccgtattcat ggccgatcat attgaaagga tgcttgctga acgatgacct
     1081 ccgaagagaa aaccgcctcc ccttctccgt atcttcggct gctcgcctat gcccgcccct
     1141 actcgcgcag gttcttcctt gcgctggggt gcatggttgc ggcctcggcc tgcaacgtcg
     1201 tgccgccgtg gctgctcaag aacgtcgtgg acgacgtcct gatcgccaag aaccttgcca
     1261 tgctcaacgc gcttcctttc atcctcgtgg cgctcttcgc cgggaaaggg atcgcctcgt
     1321 acgggcacca gtacctgatg aactgggtag ggcaaaacgt cgtgatggat ttacgccttc
     1381 ggctctacga ccatctccag cggatgtcgc tgaagtacct ctacggcaag cgtctcgggg
     1441 agctgatgtc gcggatcacg aacgacgtga ccatcctcca gaacctggtc acatccgtac
     1501 tggtcaatct tgtagtgcag gc
//
LOCUS       DAOSWW010000757         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_73865_length_1521_cov_3.439291, whole genome shotgun sequence.
ACCESSION   DAOSWW010000757 DAOSWW010000000
VERSION     DAOSWW010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73865_length_1521_cov_3.439291"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK442_12745"
     CDS             complement(<1..351)
                     /locus_tag="PK442_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOO88564.1"
                     /translation="MNDKEQQQIKTSLVVTGMTCATCSRMVERSLGKVEGVVFAAVNL
                     ATETAFVVSDREIPKEELVQAVEKAGYSVSEERAEDLEKNRYLKIKKNLALSWLVTAP
                     LMMLMVFHMAGQHVP"
     gene            complement(462..662)
                     /locus_tag="PK442_12750"
     CDS             complement(462..662)
                     /locus_tag="PK442_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOO88565.1"
                     /translation="MAKFVFSVPDISCNHCKMRISKALEAAGMKEFEVSVESKKVTAE
                     AQDAAKVQAVIEDAGYDATLEA"
     gene            976..>1521
                     /locus_tag="PK442_12755"
     CDS             976..>1521
                     /locus_tag="PK442_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88566.1"
                     /translation="MRRVMADKEMKSAMHTGRSVKLLRYFAVSGAALLMFWSVPAVEA
                     KTWTVTTTADSRMKEGSLRYIVAKATGGDTILFSSGGANLTSEIVLDKSLALVGPATI
                     KQTGWERVFSIPAGTTVTMENLTITGGSADYYGGGIYNEGMLTMTGCTVSDNRSARDG
                     GGVYSGVYDYSTQTRTGTLTLT"
BASE COUNT          339 a          417 c          422 g          343 t
ORIGIN      
        1 cggaacgtgc tgcccggcca tgtgaaagac catcagcatc atcagcggcg ccgttaccag
       61 ccacgagagc gcgaggtttt tctttatttt caggtagcgg ttcttctcaa ggtcctcggc
      121 gcgctcctcc gagaccgagt aacccgcctt ctcgacagcc tgtacaagct cctccttggg
      181 tatttcgcgg tcggacacca cgaatgccgt ctccgtggcc aggttcaccg ccgcgaagac
      241 cacgccctcc accttgccga gcgatcgctc gaccatacgg gaacatgtcg cgcacgtcat
      301 ccccgtcacg acgaggctcg tcttgatctg ctgctgctct ttatcgttca tggatttttc
      361 ctccaaatac aagattgtgc ccggaaccgg gtattcgcgg acgagaggcc ttacggaaaa
      421 ggcgtttctc gtccgcgaac gtataccggt ccttatcggc gctacgcttc aagtgttgcg
      481 tcgtaccctg cgtcctcgat gaccgcctgt accttggccg cgtcctgcgc ctccgcagtc
      541 actttcttcg actccacgga gacttcgaac tccttcattc ccgcagcctc aagggccttg
      601 gatatgcgca tcttgcagtg gttgcaggag atgtcgggaa cggaaaaaac aaacttcgcc
      661 ataggaatgc cctccttgtg atcgtatgtt tgataggata aactctatca cttttgtagt
      721 atataccact tggggtattt acgccagagg gctgaaaaag aaattaaaaa atagatactc
      781 tctacggagc accgaagaat ctcttgaacg cgaatgtaga ggcgatcaag gggagcggga
      841 attctgtccg ctttcggagc ataatttttt ttgttttttt tgggaagact aagcagattt
      901 tttaatttcc cccttgactc ttacacggtg tgaaggttta tcatgattct gcgatagaaa
      961 gacatacccc gcctcatgag acgagtcatg gcggacaagg agatgaaatc ggcaatgcat
     1021 acgggcaggt cggtaaaact tttaaggtat ttcgctgtat cgggggcggc gctcctgatg
     1081 ttttggagcg tgcccgcagt cgaggcgaaa acgtggacgg tgaccactac ggccgattcg
     1141 aggatgaagg aggggtcgct tcgctacatc gtcgcgaaag cgaccggagg cgatacgatc
     1201 ctcttctcca gcggcggagc caacctcacc tcggagatcg tcctggacaa gagtctcgcg
     1261 ctcgtaggcc cggcgacgat caaacagacc gggtgggagc gtgtcttctc catccccgcc
     1321 gggacgacgg tgacgatgga gaacctgacg atcaccggag ggagcgccga ttactacggg
     1381 ggaggaatat acaacgaagg aatgctgacg atgacgggct gcacggtctc cgacaaccgc
     1441 tccgcaagag atggaggagg cgtttatagc ggcgtctatg actacagcac gcagaccagg
     1501 acgggcacgt tgaccctgac g
//
LOCUS       DAOSWW010000758         1517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_74206_length_1517_cov_3.276334, whole genome shotgun sequence.
ACCESSION   DAOSWW010000758 DAOSWW010000000
VERSION     DAOSWW010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74206_length_1517_cov_3.276334"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PK442_12760"
     CDS             complement(<1..376)
                     /locus_tag="PK442_12760"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOO88567.1"
                     /translation="MARIAFPYDGFGDMVIPDENLLAVLAPRTATPPTGTDLDEVRRA
                     LAEPIASPGLKDMVKQGDSALLLIDDNTRNTPAARILPLVLEELAAGGVQIQDTAILI
                     ALGTHRPVTGAEVEAKVGAELAG"
     gene            758..>1517
                     /locus_tag="PK442_12765"
     CDS             758..>1517
                     /locus_tag="PK442_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOO88568.1"
                     /translation="MNQMSFLGFLALFLFCFVLSAFFSASEMAYSGINKIRVKRFVEE
                     GRPGAVKAQELLRDFNGTLSAILIGGNIVDIFMTALATAVLTTLFGPIGAVYSTLLMT
                     CLIILFGEILPKAFVKDRSEEFALSAAGMLHLLVRLFRPFTNFTRKLAAVLRRWAPGP
                     ADNLPGVTHDELLSIVDAMHEEGVLPGAEQELIGNAINFNELEVWEVQTPRVDLFAIN
                     IREPLENVRNLVVKNHYSRVPVYEGTVDNIVGFLS"
BASE COUNT          291 a          401 c          452 g          373 t
ORIGIN      
        1 gtccggcgag ttccgcgcca acctttgcct ctacctcggc gcccgtcacg gggcggtgtg
       61 ttccgagggc gatcagaatg gccgtgtcct gaatctgtac gccgcctgca gccaactcct
      121 ccagaacaag cgggaggata cgagcggcgg gggtgttgcg ggtgttgtcg tcgatgagca
      181 gcaaagccga atccccctgc ttcaccatgt ccttcagccc gggggaggcg attggctcgg
      241 caagagcccg tcgtacttcg tcaagatcag ttcccgtcgg gggcgtcgca gttcgtggag
      301 cgagcaccgc cagaaggttc tcgtcgggaa tcaccatatc gccgaaaccg tcatagggaa
      361 aggcgattcg agccatggcg cacctccttc tcgattttcg cggggatgtc gtcttggtat
      421 attgcttcgc ctcctaaaaa tgacgcctgg aggcggggtg ttttgtcagg cgaaacacta
      481 tcccgcgctt ttttctatgt tccattataa tgccttttca gcgttttatg cgcgaagagg
      541 gcgaaaagaa tttgctataa gaagaccgta tttcggaagg aagtttgctc gtgctatgat
      601 gtgctccgga ggtgcgtacg atgtatgcgg taaaaataag aaatgaaacg cttgtgcttg
      661 ttttggggcc gttcttctct cttcctgcga ttagagcgta tggggagaga tgttccgggc
      721 agtgtgcgga atgcatgcgc gcgggagggg ttgtgtcatg aatcagatgt cattcttggg
      781 ttttctcgcc ctctttctct tctgttttgt tctctcggct tttttttccg cttcggagat
      841 ggcatattcc ggcatcaaca aaatcagggt gaagcgcttc gtcgaggagg ggcgtccggg
      901 cgcagtaaag gcgcaggagc tgctgcgcga tttcaacggg acgctttcgg caattctcat
      961 cggcgggaat atcgtcgaca tctttatgac ggcgctggca actgctgtgc tgacaacgct
     1021 tttcggcccc atcggcgcgg tgtattcaac tcttttgatg acgtgtctca tcatcctctt
     1081 cggcgaaata ctgccgaaag cgttcgtaaa agaccgctcc gaagagtttg cgctctccgc
     1141 cgcggggatg ctgcatcttc tggtgcgcct tttccgcccc tttacgaact tcacccgcaa
     1201 gcttgcggca gtcttacgac gctgggcgcc cggtcctgcc gacaatcttc ccggcgtgac
     1261 ccacgacgag ctgctctcga tcgtggatgc gatgcacgaa gagggagtgc tgccgggggc
     1321 ggagcaggag ctcatcggga acgccatcaa cttcaacgag ttggaggtgt gggaggtgca
     1381 gacgccgcga gtggatcttt tcgccatcaa catcagagag cctctcgaaa atgtccgcaa
     1441 tctggtggtc aaaaatcact attccagagt tccggtctac gaaggaacgg tggacaacat
     1501 cgtcggtttc ctcagcg
//
LOCUS       DAOSWW010000759         1517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_74214_length_1517_cov_2.891929, whole genome shotgun sequence.
ACCESSION   DAOSWW010000759 DAOSWW010000000
VERSION     DAOSWW010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74214_length_1517_cov_2.891929"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="PK442_12770"
     CDS             <1..1248
                     /locus_tag="PK442_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO88569.1"
                     /translation="GDLSQYSSKQNIKEKMEEIYGQDKDYMNEGHALWQFANDVKPGD
                     VVFVKKGENMIVGRGEVTSEYMFDASRSEYQHIHEIDWTHSGEWSAEKDLPVKTLTDF
                     TPYTEACRLLEEKFEGDVVIEDDPPKTFARYTQDDFLNDVFMSEDRYVTLKNLLLKKK
                     NIILQGAPGVGKTYAAERLAYSIMEEKDTNRVMTIQFHQSYSYEDFIMGYRPTENGFS
                     MGKGPFYSFCKKAEPDDREHFFIIDEINRGNLSKIFGELLMLIENDKRGKSIRLLYSD
                     EQFQVPKNVHIIGMMNTADRSLAMIDYALRRRFAFFEFEPAFASTGFKRYQEFVANEK
                     FDFLVQTVCSMNTAITDDPSLGSGFRVGHSYFCAKDPADVDEIWLSDLVEYELIPLIK
                     EYWFDDLSKVHEWSGKLRGALRV"
     gene            1232..>1517
                     /locus_tag="PK442_12775"
     CDS             1232..>1517
                     /locus_tag="PK442_12775"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="McrC protein together with McrB forms the McrBC
                     restriction system; recognizes N4- and C5-methylcytosine
                     (and 5-hydroxy-methylcytosines); appears to act against
                     5-methylcytosine preceded by a purine residue; MrcC
                     modulates the specificty of McrB and has DNA cleavage
                     activity; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HOO88570.1"
                     /translation="MRSVSENIRIRNVYYMLAYAYHALNEAGCRSIETEDFKNVHDLL
                     AAILINGVAQQIKRGLNRDYLSNKEAISSLKGKLIVSESVKRHTLLKKRMI"
BASE COUNT          401 a          409 c          412 g          295 t
ORIGIN      
        1 ggcgatctct ctcagtactc ctccaaacaa aacatcaagg agaaaatgga agagatctac
       61 ggacaggata aggactacat gaacgaaggc catgcgctct ggcagttcgc caacgatgtg
      121 aagccgggag acgtcgtctt cgtcaaaaag ggcgaaaaca tgatcgtcgg gcgcggggag
      181 gtgacctcgg aatacatgtt cgatgcatcc cgctccgaat accagcatat tcacgagatc
      241 gactggacgc attcgggtga atggagcgcc gaaaaggacc tgcccgtcaa aacgctcacc
      301 gacttcacgc cctacaccga agcgtgtcgg ctgttggaag agaagttcga aggggatgta
      361 gttatcgagg acgatccgcc gaaaaccttc gcacgctaca cacaggacga tttcctgaac
      421 gatgtcttca tgagcgaaga ccggtacgtc acgctgaaaa acctcctgct caagaagaag
      481 aacatcatcc ttcagggagc gccgggcgtg ggaaagacgt acgcggcgga gcgccttgcc
      541 tactcgatca tggaggagaa ggacacgaac cgggtcatga cgattcagtt ccaccagagc
      601 tacagctacg aggatttcat catggggtat cgcccgacgg agaacggttt ttcgatgggg
      661 aaggggccgt tctacagctt ctgcaaaaag gcggaaccgg acgaccgcga gcacttcttc
      721 atcatcgacg agatcaaccg cgggaacctc agcaagatat tcggcgaact cctgatgctc
      781 atcgagaacg acaagcgcgg aaaatccatc cgcctcctct attcggacga gcagtttcag
      841 gttcccaaga atgtacatat catcggcatg atgaacacgg cggatcgcag cttggccatg
      901 atcgactatg cgctgcgccg gcgtttcgca tttttcgaat tcgagcccgc cttcgcatcg
      961 accggattca agaggtatca ggaatttgta gcgaatgaaa agttcgactt tctggtgcaa
     1021 accgtctgct ccatgaacac cgccatcacc gacgatccgt ctctcgggag cggttttagg
     1081 gtagggcaca gctacttctg cgccaaagac cccgccgacg tcgatgaaat ttggctctcc
     1141 gatctggtgg aatacgaact gattccgctc atcaaagaat actggttcga cgacctctcc
     1201 aaggtccacg aatggagcgg gaagctgcgc ggtgcgctcc gtgtctgaga acatccgcat
     1261 cagaaacgtc tactacatgc tggcctacgc ctaccacgca ctgaacgagg cggggtgcag
     1321 aagcatcgag accgaggatt tcaagaacgt tcacgacctt ctggccgcga ttctgattaa
     1381 cggcgtcgcg cagcagataa aacgcggact gaaccgggat tacctttcca ataaggaagc
     1441 gataagcagt ctgaaaggga agctaatcgt ctcggagtcc gtcaagcgcc atactctctt
     1501 gaaaaaacgc atgatct
//
LOCUS       DAOSWW010000760         1513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_74582_length_1513_cov_1.470508, whole genome shotgun sequence.
ACCESSION   DAOSWW010000760 DAOSWW010000000
VERSION     DAOSWW010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74582_length_1513_cov_1.470508"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PK442_12780"
     CDS             <1..833
                     /locus_tag="PK442_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88571.1"
                     /translation="VRQKAAIFHSTVSLEKEWPEGDYYVEARLDGEKKMKTVRFSVKK
                     GEAVSSVSAGGVKMPPAAKEKQDTREQVYFTGKDTALEKEERARSETKLAEKEAVFRK
                     NPADISAVLDLADAYAAMNDAASASLALALYKGLAESSPSDMILARLADAYARLSLFD
                     RAFTTALRRSWNPLVSPEGPVSQILLLAGAGGDWERGIDCLQQILAGREDEREVTLLA
                     LAALYVEKSRVLSEKEKRLRDEYRSAAAACLDEAEASLPRSSGMWENLLRLRKELEEL
                     "
     gene            830..1309
                     /locus_tag="PK442_12785"
     CDS             830..1309
                     /locus_tag="PK442_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88572.1"
                     /translation="MSRVRINGVRLACCLSLLLSALFFPLSSSLASEDASLLKAAERV
                     ASGKGSSADEALLFMENRRVNHLAMEGKLDGSVYQKTQELYDGKNRALAAEAAREAGF
                     ATEAGGKKIYKPGTDTDIQMTGSDLTAADVEKARRAYNKRVEAYLRGSGLKIDEGTN"
     gene            1334..>1513
                     /locus_tag="PK442_12790"
     CDS             1334..>1513
                     /locus_tag="PK442_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88573.1"
                     /translation="MPSPSRMKNEAEFAKAAALINGDGGNMYVSPLATGAQAKLDAGD
                     PLSVAEGQAYAEEMKA"
BASE COUNT          338 a          411 c          488 g          276 t
ORIGIN      
        1 ctgtgcgcca gaaagcagca atcttccatt cgaccgtttc gctggaaaag gagtggcccg
       61 agggtgatta ttacgtagag gccaggctgg acggagaaaa aaagatgaag acggtcagat
      121 tctcggtgaa gaagggcgag gctgtttctt cggtaagcgc cggcggagtg aagatgcccc
      181 ctgcagcgaa ggaaaaacaa gatacgcgcg agcaggtcta cttcaccgga aaggataccg
      241 ctctcgagaa agaggaacgt gcccggagtg aaacaaaact cgcagaaaaa gaagccgtat
      301 tccgtaagaa ccccgccgac atatccgccg ttcttgatct cgcggacgcc tatgccgcaa
      361 tgaacgacgc tgcgtcagcc tcgcttgcgc ttgcactgta caaaggtctg gcggaatcct
      421 ctccgtccga tatgatactt gcccgccttg cggatgctta tgcgcgtctc tccctcttcg
      481 accgagcgtt cacgacggcg cttcgacgtt cgtggaatcc gctcgtttct ccggagggac
      541 ctgtctcaca gattcttctt ctcgccgggg ccggcgggga ctgggaacgt ggtatcgatt
      601 gccttcaaca aattctggcc gggagggagg atgaacgcga ggtcactctt ctggccttgg
      661 cggctctcta tgtagagaag tccagagttt taagcgaaaa agaaaaaagg ctgcgcgacg
      721 agtatcgaag tgcggcggct gcctgtctcg acgaagccga ggcctctctt ccccgctctt
      781 ccggtatgtg ggagaatctt cttcgcttgc gaaaggagct tgaggagcta tgagcagagt
      841 gcggataaat ggggtacggc ttgcatgctg tctgtcgctg cttctgtcgg ctctgttttt
      901 tcccctctct tcctcccttg catcggagga cgcaagcctg ctgaaggcgg cggagagggt
      961 cgcttcggga aaagggagct ctgccgacga ggcgctcctc ttcatggaga accggcgggt
     1021 gaaccatctc gcgatggagg ggaagctgga cgggagcgtc tatcaaaaga cgcaggagct
     1081 ctacgacgga aaaaacagag cgctggccgc ggaagccgcg cgcgaggcgg gattcgccac
     1141 ggaggccggc ggtaagaaaa tctacaagcc cggcacggac acggatatcc agatgacggg
     1201 gtccgacctt accgcggctg atgtagagaa ggcgcggcgg gcctataaca agcgggtcga
     1261 ggcgtacctc cgcgggagcg ggctgaaaat agatgaggga accaattgag ccgcgcggac
     1321 ggagacggac atcatgccct ctccctcgcg gatgaagaac gaagcggagt ttgccaaggc
     1381 cgccgcgctc atcaacgggg acggcggcaa tatgtacgtc tccccccttg ccaccggggc
     1441 gcaggcgaag ctggacgccg gagaccccct gagcgtcgcc gaggggcagg cgtacgcgga
     1501 agagatgaaa gcc
//
LOCUS       DAOSWW010000761         1511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_74679_length_1511_cov_5.030907, whole genome shotgun sequence.
ACCESSION   DAOSWW010000761 DAOSWW010000000
VERSION     DAOSWW010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74679_length_1511_cov_5.030907"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PK442_12795"
     CDS             complement(<1..747)
                     /locus_tag="PK442_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOO88574.1"
                     /translation="MILSKIIEEYAARYSSPLRLHNQAIDGGTDVSVVHVGCNSLEIK
                     KDELFCCIRGEHNDGHSFAGDAVQKGASTLLVERPLPLETPQIVSSNVRRDMGRLSSI
                     VYGDPANTLKMFAVTGTNGKSTTTWIIRHLLQSIGIRTGLLGTIVYNDGIEEKDAGRT
                     TPESCDIQLMLLRMLQNGCGACVMETSSHGLSFGRIEGCSFDAAVFTNLSEEHLDYHG
                     TLENYFSAKSRLFSDYMKQNWAGGANGDDAF"
     gene            complement(755..>1511)
                     /locus_tag="PK442_12800"
     CDS             complement(755..>1511)
                     /locus_tag="PK442_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO88575.1"
                     /translation="YRIKIADGHISEAGKNGFGRLSVPEILAKSSNTGMAQIGIRVKP
                     FNMYHSVREWGFGKPTGIELPGAEDGLLSSPEQWRGVVPSNIAIGQGLAITPLHLASA
                     ISAIANGGNLLRPHIVSKVVDSSGNIVYNEERQFLRTVLSPQVALWLQRAMVDTVVKG
                     TGRNGAVPGISIAAKTGTAQIAERGEYAKGRWVSSFAGFWPSSDARYVVLVVIGEPSK
                     GKYYGGDVAAPVFRRIVEDMEQIGLLAAVKTEG"
BASE COUNT          372 a          405 c          331 g          403 t
ORIGIN      
        1 aaatgcatcg tcaccattcg ctccccccgc ccaattctgc ttcatgtagt cagaaaacag
       61 acggctcttt gccgaaaagt aattttcgag cgttccgtgg taatcaagat gctcctcgct
      121 gaggtttgta aagactgcgg cgtcaaacga acacccttca attcttccga aagagagacc
      181 atgtgaggat gtctccataa cacacgcacc gcagccattt tgcagcattc tcaatagcat
      241 cagctgaata tcgcagcttt ccggggttgt tcttccggcg tctttttcct caataccgtc
      301 attgtagact attgtcccta aaaggcctgt ccgtattcct attgattgca gaaggtggcg
      361 tattatccac gtagtcgtgc tcttgccgtt tgttccggta acagcgaaca ttttcagagt
      421 gttggccgga tctccgtaga caattgaaga taaacgtccc atgtctcttc gaacgttgga
      481 tgacacgatt tgaggtgttt ccagtggaag agggcgctct actaaaagag ttgaagcccc
      541 cttttgaacg gcgtctccag cgaaggaatg gccgtcatta tgttcgcctc gaatgcagca
      601 aaacagctcg tctttcttta tttccaagct gttgcaaccg acatgtacaa cggaaacatc
      661 cgttcctcca tcgatcgcct gattatgcag ccgtaacgga gatgaataac gtgcggcata
      721 ttcttcaatt atcttcgaga gaatcatcaa ggtatcatcc ttccgttttc actgccgcta
      781 acaatccgat ctgttccatg tcctcaacaa ttcgcctaaa tacaggcgct gcaacatctc
      841 cgccataata ctttcccttt gaaggttctc caatgacaac aagcacaaca tagcgagcat
      901 cgctcgaagg ccaaaaacct gcgaaggagg aaacccatct gcccttggcg tattctcctc
      961 tttcagcgat ctgagcagta cctgtcttgg cagcgattga aattcctgga actgcgccat
     1021 tccgtcctgt gcccttgaca acagtatcga ccatcgctct ctgaagccat aacgcgactt
     1081 gaggagacaa cacagtccga agaaactgcc tctcttcatt gtatactata tttcctgaag
     1141 aatctactac cttggaaacg atatgtggac gcaggagatt gcctccgttc gctatcgcag
     1201 agattgcgga tgcaaggtgc aacggcgtaa tagcgaggcc ctgccctatg gctatattcg
     1261 aaggaacaac ccctctccac tgctccggtg atgataaaag gccgtcctcg gctccgggaa
     1321 gttcaatccc cgtcggtttg ccgaatcccc actcgcgaac tgaatgatac atgttgaatg
     1381 gttttacgcg tatcccaatt tgcgccattc ctgtattcga ggattttgcg agaatttcgg
     1441 ggacggaaag ccttccgaaa ccgttttttc cggcttcgga tatatgaccg tcggcaattt
     1501 tgatcctata g
//
LOCUS       DAOSWW010000762         1511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_74696_length_1511_cov_3.627060, whole genome shotgun sequence.
ACCESSION   DAOSWW010000762 DAOSWW010000000
VERSION     DAOSWW010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74696_length_1511_cov_3.627060"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..>1511)
                     /gene="ptsP"
                     /locus_tag="PK442_12805"
     CDS             complement(179..>1511)
                     /gene="ptsP"
                     /locus_tag="PK442_12805"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOO88576.1"
                     /translation="SERVIKLLLQAGGMDFTQLQEKAILVTRDFTASDMSQIQREMTL
                     GVASEVGGRTSHAAIIARTMQIPSVMGAHGLLSTVGNGDLLILDGETGEIFVNPDEET
                     LARYKEKQERYECFLGRLSEIRGKETVTLDGVKVELGANVGSSRDLDEVLKNDGEAVG
                     LYRTEFLYLESPMLPTEDEQFEAYKSVVEGMDGRPVVIRTLDIGGDKKLDYLSIPEEE
                     NPFLGYRAIRLCLDRQEIFKVQLRAILRSSAYGNVRILFPMISCLEELREAKVILENT
                     KDDLRQRKIPFDEGVLAGIMVEVPSTAVLSDHFAKESDFFSIGTNDLIQYTVAVDRGN
                     DKLAPLYSSYHPAVLRLVKTVIDNGKQAGIHVGMCGESAGDPKLIPILIGMGLTEFSM
                     SPPSILQARWILRNLRKSDLEEAARHALSLGTAAEVEAYCGTLLQSLDLCR"
BASE COUNT          259 a          472 c          481 g          299 t
ORIGIN      
        1 tcgtccccca ggttcagcac ccgttcgatg atcgccgtgc ggttgcgccg aaagtccacg
       61 gcagaccact ccgcgtccca gaagagggga cgcagaaact cggggagtcc ggaatgattc
      121 gtcccgcgga ttgatgtgct catgacggcg atcgggccgc gcgcggccgg tcctccgttc
      181 agcggcagag gtcgagcgac tgaagcagcg tgccgcaata cgcctccacc tccgcagccg
      241 tccccagcga aagagcgtgc cgcgccgctt cttccaggtc gctcttccga aggttccgca
      301 gaatccaccg ggcttgcaga atggacggag ggctcatgct gaactccgtc agccccattc
      361 cgatcagaat cggaatcagc ttgggatctc ccgccgactc gccgcacatc ccgacgtgga
      421 taccggcctg cttgccgttg tcgatcacgg ttttcacgag ccggagcacc gccgggtggt
      481 aggatgagta gaggggagcc agcttgtcgt ttccgcggtc gaccgccacg gtgtactgga
      541 tgaggtcgtt cgtaccgatg ctgaagaagt cggactcctt cgcgaagtgg tcggagagca
      601 cggcggtcga gggaacctcc accatgatgc ccgccaggac gccctcgtcg aacggaatct
      661 tccgctgacg gagatcgtcc ttcgtgtttt cgaggatgac cttcgcttcc cggagctcct
      721 caagacagga gatcatcggg aagaggatgc gcacgttgcc gtaggcgctc gatcggagga
      781 tcgcccggag ctgcaccttg aatatctcct gccggtccag gcagaggcgg atggcgcggt
      841 agccgaggaa cgggttctcc tcctccggaa tggagaggta gtcgagcttc ttgtcgccgc
      901 cgatgtcgag cgtcctgatg accaccggac ggccgtccat cccctcgacg acgctcttgt
      961 aggcttcgaa ctgctcgtcc tcggtgggga gcatcgggct ttcgaggtag aggaactccg
     1021 ttcgatagag accgacggcc tcgccgtcgt tcttcagcac ctcgtcgaga tcgcgggagg
     1081 aaccgacgtt tgcgccaagc tcgaccttca cgccgtcgag cgtgacggtc tctttcccgc
     1141 ggatttcgga gagacgtccg aggaagcatt cgtaccgttc ctgcttttcc ttgtagcgcg
     1201 ccagcgtctc ctcgtcgggg ttcacgaata tctcccccgt ctcgccgtcg aggatgagca
     1261 ggtcgccgtt gcctaccgtg ctgagcagcc cgtgcgctcc catcacggag ggaatttgca
     1321 tcgtgcgggc gatgatcgcc gcatgggagg tcctgcctcc gacttcggag gcgactccga
     1381 gcgtcatctc gcgctggatc tgcgacatat cggacgctgt gaagtcgcgg gtgacgagga
     1441 tggccttctc ctggagctgg gtgaagtcca ttccgcccgc ctgaaggagg agcttgatca
     1501 ctcgttcgga g
//
LOCUS       DAOSWW010000763         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_74804_length_1510_cov_2.945017, whole genome shotgun sequence.
ACCESSION   DAOSWW010000763 DAOSWW010000000
VERSION     DAOSWW010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74804_length_1510_cov_2.945017"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1295
                     /locus_tag="PK442_12810"
     CDS             75..1295
                     /locus_tag="PK442_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOO88577.1"
                     /translation="MSSIWGKNFSNIARNNRPSPIREMLAVIKQPGMISFAGGMPAPE
                     IFPIDQFYEGAHILKDDGANLLQYGTTEGYPPLKEFLASWTAPRMGRTVGLDEMLITT
                     GSQQALDLFAWSMIDEGDYVVVEDPSYLAALTVFFNHGGRFLGVPMDEEGMQVDLLPG
                     IIEKARSEGKRIKFIYTIVNFQNPGGSTMSVPRRKQLVEISKKYNIPVFEDDPYGYVR
                     YDGEHLPSIFSFHDEEGVVYAGSFSKILSPGTRVGWIAGPKEIIRNLTVFKQGTDLCS
                     SPITQALVAEYCAKGYLDSHLPVIIDNYRVKRDAMEKSFEKHLAPLGVTWVKPEGGFF
                     YWLNVPSVNTNDLFKKAIEKKVAFVPGAPFCPVEGSGLHNARLCYTFPSPEIIEDGVA
                     RLAEAIGELAIPAL"
BASE COUNT          366 a          419 c          369 g          356 t
ORIGIN      
        1 ctcttttcga gttttttttg gagcttacgc tcagaaagac tcaaatttac catatcatca
       61 agggggcgat ttgtatgagc agtatctggg gaaagaactt cagcaacatc gcgaggaaca
      121 accgtccttc gccaattcgc gagatgctcg ccgttatcaa gcagcccggt atgatctctt
      181 ttgccggggg gatgcctgcg cccgaaatct tccctatcga tcagttttac gagggagcgc
      241 acattctgaa ggatgacggc gcaaacctcc ttcagtacgg caccaccgaa gggtaccctc
      301 cgctcaagga attcctggct tcatggaccg ccccccggat gggacgaacg gtcggtctcg
      361 atgagatgct catcactaca ggctctcaac aggcccttga tcttttcgca tggtccatga
      421 tcgacgaggg cgactacgtc gtcgtcgaag acccctcata cctggctgcc ctcactgtct
      481 tctttaatca cggcggcagg ttcctcggcg ttccgatgga cgaggaggga atgcaggtcg
      541 atctccttcc gggaatcatc gaaaaggcac gctccgaggg gaaaagaata aaattcatct
      601 acaccatcgt caatttccaa aatccgggcg gttcgacaat gtccgttccc cggcgcaaac
      661 agctcgtgga gatttcaaaa aaatacaata tccctgtctt cgaggatgac ccttacgggt
      721 acgttcgtta tgacggcgag catctccctt caatcttctc attccacgac gaggagggcg
      781 ttgtctatgc cggttccttc tcgaagatac tctcgcccgg cactcgcgta ggctggatcg
      841 ccggccccaa agagatcatt cgcaacctga ccgtttttaa gcaggggacg gacctctgct
      901 ccagccccat tacacaggcg ctcgtcgccg aatactgcgc caaaggctat cttgacagcc
      961 atctgcccgt tatcattgac aattaccggg tcaagcgcga cgctatggag aaaagctttg
     1021 aaaaacacct cgctcctttg ggcgttacct gggtcaaacc cgagggaggc tttttctatt
     1081 ggctgaacgt gccttcagta aatacgaatg accttttcaa aaaagccatt gagaaaaaag
     1141 tcgccttcgt tcccggcgct cctttctgtc ctgtagaggg gtccggactg cacaatgcac
     1201 gcctttgcta caccttcccg agtcctgaga tcattgagga cggcgtggca cgtttggcgg
     1261 aggccattgg agaactcgcc attcctgcgc tgtaaagcag aaagaaacaa aagccgcacg
     1321 aaagactgct ttactctctc ttcgctatac tctctccatc ttgaacagaa aaacctctcc
     1381 cgaaatgaag atttagggag aggttttcgt ttttagtaca gcgcttttgc ggtacaatac
     1441 gggacacaga cagcaaaaat ccactttgag aggaggaaga acatgcgccc ggtcaatgac
     1501 tcatttcaag
//
LOCUS       DAOSWW010000764         1508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_75012_length_1508_cov_1.883689, whole genome shotgun sequence.
ACCESSION   DAOSWW010000764 DAOSWW010000000
VERSION     DAOSWW010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75012_length_1508_cov_1.883689"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PK442_12815"
     CDS             complement(<1..1164)
                     /locus_tag="PK442_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88578.1"
                     /translation="MGKRRIAPTFNRLMRESVFVQMLVVLVLLFILVFSLWGFWYAGV
                     IPLAGLFLTKGFTLGNKLVAEGREGRRRFRMKGGHAVILGWDKTGPGILRRSLEENDR
                     ALVFSSLGTGNILSDMAAANIRPDKKLFLYNGNYADGDELNELSLESASRLYILGDEG
                     DPSRDSRNIETAVTILDILEKTSPSTPLDCFFQAYDLHIFNMMQGSDFFAKADRRRLN
                     LRLFNFYEEWAGRLWSFPPQDGEAAYPPLAYDPQRLSEGGNVHLVIAGFGTMGQALAV
                     QAIRICHYANGAKTRITAIDRDMERKKEEFVRCFPILDENGQCMLHDVEVNCIAASLE
                     SPEIRRFLKQTVADEKQLPTVVVCFSDVDGSLSAALSLPLEIKKSRIPVLIRQE"
     gene            complement(1247..>1508)
                     /locus_tag="PK442_12820"
     CDS             complement(1247..>1508)
                     /locus_tag="PK442_12820"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HOO88579.1"
                     /translation="LDDCDKVVAVFSPSYLDSKVCIEEFNIAKLRHTEEDCGVLLPIF
                     LYSANLPDYMLRYQYFDCRENDRSKLADACKKIIRKIEEERK"
BASE COUNT          312 a          421 c          419 g          356 t
ORIGIN      
        1 ctcctggcgt atcaggaccg gaattcggga tttcttgatc tcgaggggaa gcgagagcgc
       61 cgccgagagg gagccgtcga catcggagaa gcagacaaca acggtcggga gctgcttttc
      121 atccgcgacg gtctgtttca ggaatcgcct gatttcaggg gattcgagcg aggccgcgat
      181 acagttcact tcaacgtcgt gcagcatgca ctgcccgttc tcgtcgagta tcgggaagca
      241 tctgacgaac tcctccttct tccgctccat atccctgtcg atcgcggtga tccgtgtttt
      301 cgctccgttc gcgtagtggc atatcctgat cgcctgcacc gccagggcct gtcccattgt
      361 gccgaatccg gcgatgacga ggtggacgtt gccgccctcc gagaggcgct gcggatcata
      421 ggcgagaggg ggatacgccg cttcgccatc ttggggcgga aagctccaca gtcttccggc
      481 ccactcctcg tagaagttga agagccgaag attcaggcgg cgtctgtccg ctttggcgaa
      541 gaagtcgctc ccctgcatca tgttgaagat gtgcaggtcg tacgcctgga agaagcagtc
      601 caggggcgtg gagggggagg tcttctccag tatgtcgagg atcgtgaccg cggtttctat
      661 gttcctgctg tcgcgggacg ggtctccttc gtcccccagg atgtagaggc gggaggccga
      721 ttcgagggag agttcgttga gttcgtcccc gtcggcgtag tttccgttgt agaggaagag
      781 cttcttgtcc ggcctgatat tcgccgccgc catatcggag aggatgttcc cggtgccgag
      841 cgaggagaag acgagcgctc tgtcgttttc ctccaggctt ctcctgagta ttcccggtcc
      901 cgtcttgtcc caccccagga tgacggcgtg accgcccttc atcctgaagc gtcgtcttcc
      961 ctcccggcct tccgcgacga gtttgttgcc gagggtaaag ccctttgtca ggaatagacc
     1021 cgcgagaggg atgaccccgg cgtaccagaa cccccacagg gagaagacta gtatgaagag
     1081 gagaacgaga acgacgagca tctgcacgaa tacgctctcc cgcatcagcc tgttgaacgt
     1141 cggggcgatt ctcctttttc ccatatcgtt acacaccatc ctttgaacgc gggcttcgaa
     1201 gagcttcagg cgcggtattc ccctcgggaa atacgcatca aagcacctat tttctttcct
     1261 cttcaatttt cctgattatc tttttacagg catccgccag tttggagcga tcattttcac
     1321 ggcagtcaaa atattgatac ctaagcatgt aatctggcaa atttgcacta tagagaaata
     1381 taggcaagag taccccacag tcttcttctg tatgccgaag ttttgcaatg ttaaactcct
     1441 ctatgcatac cttagaatcc aagtacgaag gggagaaaac tgccacaact ttatcacaat
     1501 catccaga
//
LOCUS       DAOSWW010000765         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_75278_length_1504_cov_2.937198, whole genome shotgun sequence.
ACCESSION   DAOSWW010000765 DAOSWW010000000
VERSION     DAOSWW010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75278_length_1504_cov_2.937198"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PK442_12825"
     CDS             <1..790
                     /locus_tag="PK442_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOO88580.1"
                     /translation="KFGFPLAGDSLNAAVRRASASEHMCLKGFHFHIGSQIFENTSHV
                     MAVEIMVRAVAEFKKEIGFVTEELNMGGGYGVEFAPDGRTPRIDSFTDAMMRTLEEQC
                     RIAGVDRPRVIIEPGRWIVSEAGITLYTVQAIKKIDGIVTYASIDGGMTDNPRPSLYG
                     AKYWAVAAGKMNREASAKVTVAGRCCESGDILIENLKTPPLERGDLLAVLNTGAYTFS
                     MASNYNRLPRPAVVLASEGRADVIVPRQSYEDLIRGESLPEHLR"
     gene            937..>1504
                     /locus_tag="PK442_12830"
     CDS             937..>1504
                     /locus_tag="PK442_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88581.1"
                     /translation="MKKSLALFGVLLLLVFAGSAFAADVIKIGHTVSLTGGASMWGQS
                     EARALDMLAKKINEAGGVLGKQIEFVRYDNRNDGVEAVNVARRLVSDGVVAVIGPAQS
                     GNSIASAPVFERGKIPMVVTTATNPYVTIDQRSNKVRPFAFRPCFIDPFQGTVAAHFA
                     VTDLGAKKGAILYDVGSDYGQWLARYFEE"
BASE COUNT          313 a          417 c          480 g          294 t
ORIGIN      
        1 gaaattcgga tttcctctcg ccggcgactc gctgaacgcg gccgtccgcc gtgcgtccgc
       61 ttcggagcat atgtgtctga aagggtttca ttttcatata ggctcgcaga tattcgagaa
      121 tacgtcgcac gttatggccg tcgagatcat ggtacgcgcc gtcgccgaat ttaagaaaga
      181 aatcggtttc gtcaccgagg aactcaacat gggcggggga tacggcgtgg aattcgcccc
      241 ggacggcagg actccccgga ttgattcttt tacggatgcg atgatgcgta ctctcgaaga
      301 acagtgccgc atagcgggcg tcgaccgtcc gcgcgtgatc atagagcccg ggcgctggat
      361 tgtctccgag gcggggatta cgctctatac ggtccaggcg atcaaaaaga tcgacggcat
      421 cgtaacctat gcgagtatcg acggaggaat gaccgacaac cccagaccct cgctctacgg
      481 ggcgaagtac tgggcggtcg ccgcgggaaa gatgaatcgc gaggcgtcgg cgaaagtgac
      541 tgtggcggga cgatgctgcg agtcaggcga catccttatc gaaaatttga aaacgccgcc
      601 gttggagcgg ggggatctgc ttgccgtgct gaataccggc gcgtacacct tctccatggc
      661 gagcaactac aaccgactcc cgcgccctgc cgtggtgctg gcgagcgagg ggagagcgga
      721 cgtgatcgtt ccccggcaga gctacgagga ccttatccgc ggagagtctc tgcccgaaca
      781 tttacgatag aactggttcc tgaatttgta cgcggaggtg gggcctgaga agaatcgttg
      841 cgggaaaagg cgggaatccg tgaaacgggc ttcgcgcggg tgaacggccc cggcgcgggg
      901 aatcacgaaa cacatcacaa ggaggtagga agagatatga aaaagagtct tgcgttgttt
      961 ggagtactgc ttctgctcgt ctttgccgga tcggcgtttg cggctgatgt catcaaaata
     1021 ggccacacgg tctccctcac cggcggcgct tccatgtggg ggcagtccga ggcccgggcg
     1081 ctcgacatgc tggcgaagaa gatcaacgag gccggaggcg ttctcggcaa gcagatcgag
     1141 ttcgtcaggt acgacaaccg gaacgacggc gtggaagcgg tcaacgtggc ccggcgcctt
     1201 gtctcggacg gtgttgtcgc cgtcatcggt cctgcgcaga gcggcaattc catcgcgtcg
     1261 gccccggtct tcgagagggg aaaaattccc atggtcgtga cgactgcgac caacccctac
     1321 gtcacgattg atcagcgctc gaacaaggtc cgtcccttcg cctttcgccc ctgcttcatc
     1381 gaccccttcc agggaaccgt ggcggcacat ttcgccgtga ccgacctcgg cgcgaaaaag
     1441 ggcgccatcc tctatgacgt gggttcggac tacggccagt ggcttgcacg ttatttcgaa
     1501 gagg
//
LOCUS       DAOSWW010000766         1500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_75612_length_1500_cov_3.025606, whole genome shotgun sequence.
ACCESSION   DAOSWW010000766 DAOSWW010000000
VERSION     DAOSWW010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75612_length_1500_cov_3.025606"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK442_12835"
     CDS             complement(<1..276)
                     /locus_tag="PK442_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88582.1"
                     /translation="MNDFFKFPSTPHLAVLLGVDVRGDKVMSEKERDEFLRHEITVEE
                     KIDGANLGISFDGGGSLLLQNRGGIVQLHTIKEATPHARRDHRRYRGF"
     gene            complement(524..>1500)
                     /locus_tag="PK442_12840"
     CDS             complement(524..>1500)
                     /locus_tag="PK442_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88583.1"
                     /translation="GTLFNDFCEKENSSTTEIDQPSPALVWEQNVAKHALDELFTFTQ
                     QYKSSQSFHELIKFAARFRFYSPFNAMLVHIQQPGAMFVAPPYRWLRDYKRQIKPGAK
                     PLVILQPMGPVMFVFDVADTEPTKNAPPLPKEVEKPFALSGRKVGRELDFIIENAKRD
                     GIRISRTHEGSQSAGSVRPAANDLKASLFFQSALDKERRPVFEAILIRYDLIINEALE
                     PNAQYVTTVHELAHLYCGHLGTPNAKWWPDRCGLSKEVREFEAESVAYLVALRAGIDS
                     ASEKYLSGYLPENKEIPPISLECVMKSAGLIEAMSSQRMKPRKQKETA"
BASE COUNT          342 a          403 c          361 g          394 t
ORIGIN      
        1 gaacccgcga tatctccgat gatcgcgccg agcatggggc gtcgcctcct ttatggtatg
       61 tagttgaacg atgcctcccc tgttctgcag caggaggctc ccgccgccgt cgaacgaaat
      121 gccaaggttg gccccgtcga tcttctcttc aacggtgatc tcgtgccgta ggaattcgtc
      181 ccgctccttc tcggacatca ccttgtcccc ccggacgtcg acacccagca gaacagccag
      241 atgaggagta gaggggaact tgaagaagtc gttcatgggg gatccatctt ccctcttgaa
      301 ccgaagaaca aagatatcgg agccatgcgg atgagtctgg tatctttgca ctccttgaag
      361 ttttgtattc gcaactgccg aagcatccgc catctcctcc tgctcctgtt atcgtctttg
      421 gaaagatgaa ccggaaactc tccggtactt tcccggcaac taccccggta cttccccggc
      481 gacttccacg gtcaggtgag ctacttgaga aaacttgcga ttgttaggca gtctctttct
      541 gttttctcgg cttcatcctt tgagaactca ttgcttcgat gagtcccgcc gatttcataa
      601 cgcattcgag gcttattgga ggaatctcct tattttcagg caagtatcca gaaagatact
      661 tttccgatgc cgaatctatc ccagctcgaa gagcaaccag gtacgccacc gattccgctt
      721 cgaactctcg tacttcttta ctcagaccgc agcgatccgg ccaccatttt gcgtttggcg
      781 tgcccaagtg cccacagtaa agatgcgcta actcatgcac cgttgtgaca tactgcgcat
      841 tgggttcgag agcctcattt atgattaaat catatcgtat tagaatggct tcgaaaaccg
      901 gtcttctctc tttatccaag gcagactgaa aaaacagtga agcttttaag tcattggcgg
      961 caggccgtac cgaacctgcc gattgcgatc cttcatgcgt acgagaaatt cgaataccat
     1021 cacgcttggc gttctcgata ataaaatcaa gttctctgcc gacttttctg ccgcttagcg
     1081 caaagggttt ttcaacctct ttcggcagcg gcggagcatt tttcgtaggt tcggtgtcag
     1141 ccacatcgaa aacgaacata accggtccca tcggttgcaa aatgaccaga ggctttgcgc
     1201 ctggcttaat ttgacgttta taatcgcgta accaacgata cggaggagcc acgaacatcg
     1261 ctcccggctg ttggatatga accagcatag cgttgaaagg cgagtagaat ctgaatcgag
     1321 ccgcgaactt tatcagttca tgaaacgatt gactgctctt gtattgctgt gtgaaggtga
     1381 acagctcgtc caaggcgtgc ttcgcgacgt tctgttccca caccaaggcc ggtgagggtt
     1441 ggtcgatttc tgttgtagag gagttctcct tttcgcagaa gtcgttgaaa agagtgccct
//
LOCUS       DAOSWW010000767         1500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_75613_length_1500_cov_3.023529, whole genome shotgun sequence.
ACCESSION   DAOSWW010000767 DAOSWW010000000
VERSION     DAOSWW010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75613_length_1500_cov_3.023529"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..>1500)
                     /locus_tag="PK442_12845"
     CDS             complement(76..>1500)
                     /locus_tag="PK442_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88584.1"
                     /translation="MMVKLGPKGVAYLMNTQTGKTMLSKFAQRTAWMGPAAMNRLSKL
                     TGMEERKIRDLASWTWNELTKERRLWGNRAAAQAGAAGGNFAGSQAGQAAKRNAEKRI
                     ADGKKLIDKLQATTKQKEFRKLALQLQQNKTAVALANDPSVPAALRAKLNKTLQEINR
                     RVDKNTARGIVNKVRSVNKKVEDFVRTELPQAGSKAGTKIDQFLRDNPGLSREDVLFA
                     NRVESFLRKNPGMRREDILVRSRTVSGVDPTKLGRDRDVYFQFVDRRGKVLGDVHHDI
                     AAPIYNQKLQAATGLSSKQLDHTVTSTWHPDSYNPGRMASDGPRRQLVDDIVKGRAAG
                     KLARPQDIRDTVAGKAKEWFDDARRLEAAGNPSAAAEAYAEGMRQLGKDYERHVAPFL
                     RSQNLDPAAALPPRLKAALDIFKKVEQGTTSGAYTPEQGLRALQSLSCRTPGGGNIPM
                     SPDKAAEDLGLFIEMMNKWMIQGR"
BASE COUNT          201 a          516 c          431 g          352 t
ORIGIN      
        1 gagagaggga aggcgcaggc ctcccgcccg ttccttccct ctctcttcat ccgctcatgt
       61 ttttgcgaac gctccttacc tcccctggat catccacttg ttcatcattt cgatgaacag
      121 gcccaggtcc tccgccgcct tgtcgggcga catcgggatg ttgcctcctc cgggggttct
      181 gcacgacagc gactgaaggg cgcggagccc ctgctccggc gtataggctc ccgacgtggt
      241 cccctgctcc accttcttga agatgtcgag cgctgccttc agccttggcg gcagcgccgc
      301 cgccggatca agattttggc ttcgcaggaa gggcgccacg tgacgctcgt agtccttgcc
      361 caactggcgc attccttcgg cgtacgcttc ggccgctgcg ctcgggttcc ccgcggcttc
      421 gagacgtctg gcgtcgtcga accactcctt ggccttcccg gcgacggtgt cgcggatgtc
      481 ctgcgggcgg gccagctttc ccgccgccct gcccttcacg atatcgtcga cgagctgtct
      541 gcgcggtccg tccgaagcca ttcttcccgg attgtacgag tccgggtgcc acgtgctggt
      601 gacggtgtgg tcgagctgct tcgacgaaag ccccgttgcg gcctggagct tctgattgta
      661 aataggcgct gcgatatcgt gatggacgtc gccgagcacc ttgcctcttc ggtccacgaa
      721 ctggaaatag acgtccctgt cgcggccgag cttcgtcgga tcgactccgg aaaccgtcct
      781 ggagcgaaca agaatatctt ctctcctcat accggggttt ttcctgagga acgactccac
      841 cctgttcgcg aagagcacgt cctctctgct cagccccgga ttgtccctga ggaactggtc
      901 tatcttcgtg cccgccttcg acccggcctg cggcagttcg gtgcgcacga agtcctccac
      961 cttcttgttg accgaccgca ccttgttcac gatcccccgc gcggtgttct tgtcgacgcg
     1021 ccggttgatc tcctgcagcg tcttgttcaa tttcgccctg agggccgcgg gcacgctcgg
     1081 atcgttggcg agcgccacgg cggtcttgtt ctgctgcaac tgcagggcga gcttgcgaaa
     1141 ctccttctgt ttcgtggttg cctgcagctt gtcgatgagc ttcttcccgt ccgcgatgcg
     1201 tttctccgcg tttcgtttcg cggcctgccc cgcctgcgaa ccggcgaagt tgcctcctgc
     1261 ggctccggcc tgcgccgcgg cgcggttccc ccaaaggcgc cgctccttcg tcagttcatt
     1321 ccacgtccag gaggcgaggt cgcgaatctt ccgctcctcc attcccgtga gcttcgagag
     1381 acggttcatc gccgcgggcc ccatccaggc cgtcctctgg gcgaacttcg agagcatcgt
     1441 cttccccgtc tgtgtattca tcaggtaggc gacgcccttg ggtccgagct tgaccatcat
//
LOCUS       DAOSWW010000768         1496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_75906_length_1496_cov_3.624566, whole genome shotgun sequence.
ACCESSION   DAOSWW010000768 DAOSWW010000000
VERSION     DAOSWW010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75906_length_1496_cov_3.624566"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1022)
                     /locus_tag="PK442_12850"
     CDS             complement(9..1022)
                     /locus_tag="PK442_12850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO88585.1"
                     /translation="MLISWNTTRRCNLFCRHCYRESGPGEDIRDELSTAEGIELIASI
                     AEAGFRLLIFSGGEPLLREDILELIAAARDRGLVPAMGTNGTLLTESVARELAAAGLR
                     GIAISVDSLDREYHDAFRGSHGAYDLTQAGIRNALAAGLRVQINLTLTEKNLEEFDKM
                     VAYYESTGVHAIHPFFLVPTGRARSMPEEELKSGAYFGMIRKILERQATTTLELKPTC
                     APQFMPMAKEMGIAQRFTRGCLAGVSYCCILPKGEVHVCPYLPVEAGNVRRTAFADIW
                     RESEVFAELRDFSRYEGRCGACEYVAICGGCRARAFSYTGNYMAEEPWCFRSGTWTTS
                     TGS"
     gene            complement(1016..>1496)
                     /locus_tag="PK442_12855"
     CDS             complement(1016..>1496)
                     /locus_tag="PK442_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOO88586.1"
                     /translation="FLAEKTLDFAARGVETEILTVDNHCDGIYLYLREKERGGSRAAE
                     MLELLTASGGNRSGVAIGAVDWEGDVHIDQFTQEIILGNVRERDFPELWRGETDSFLH
                     RLRDRKRHLRGRCRTCGWLEQCNGNLRARALAAGDFWGPDPSCYLTGDEIASGGATC"
BASE COUNT          270 a          506 c          431 g          289 t
ORIGIN      
        1 cagattgatc agcttccggt cgacgtcgtc catgttccgc tcctgaagca ccagggctcc
       61 tccgccatat agtttccagt gtaggagaac gcgcgcgctc tgcacccgcc gcagatcgca
      121 acatactcgc atgcgccgca gcgcccctcg tagcgcgaga agtcgcgcaa ctccgcaaag
      181 acctcgctct cgcgccaaat atcggcgaag gccgttcttc tgacgtttcc tgcctccacc
      241 ggcaggtatg ggcagacgtg gacctcgcct ttcggcagaa tacaacagta ggagactccg
      301 gcgagacagc cgcgcgtaaa acgctgcgcg attcccatct ccttcgccat cggcatgaac
      361 tgcggcgcgc aggtgggctt cagttcaagc gtcgtcgtcg cctgtcgttc caggatcttc
      421 cgtatcatcc cgaaatacgc gccggatttc agttcctcct ccggcatgga acgcgcgcgg
      481 cctgtgggaa caaggaagaa cgggtgaatg gcgtgcacgc ccgtcgattc gtaataagca
      541 accatcttgt cgaattcttc caggttcttt tccgtgagag tcaagttgat ctgcaccctc
      601 agccctgcgg caagggcgtt tcgaattcct gcctgcgtca ggtcataagc cccgtgagag
      661 cccctgaacg cgtcgtggta ctccctgtcc aggctatcca cactgatcgc gatccccctg
      721 agaccggccg cggcaagctc gcgcgcgacc gattccgtca gcagcgtccc gttcgtcccc
      781 atcgccggga cgaggccgcg gtcgcgggct gcggcgatca gttcgagaat atcctcccga
      841 agcagcggtt cacccccgct gaagattagg agacggaacc ccgcctccgc gatagaggcg
      901 atcagttcga ttccctccgc ggtggacagc tcgtcccgga tatcctctcc cgggccggat
      961 tctctgtagc agtgcctgca gaagaggttg cagcggcgcg tcgtgttcca ggagatcagc
     1021 acgtcgcccc tccggacgcg atctcgtcgc cagtgaggta acaggacgga tcgggacccc
     1081 aaaaatcccc ggcggcgagc gccctggccc ggagattccc gttgcactgt tccagccacc
     1141 cgcaggtgcg gcaccggccg cggagatgcc gcttccggtc gcgcagacga tgcagaaatg
     1201 aatccgtctc tccccgccag agctcgggga agtccctctc gcgaacattg ccgaggatga
     1261 tctcctgcgt gaactggtct atgtggacat ccccctccca gtcgaccgcg ccgatcgcga
     1321 cgccggagcg gttgcctccg cttgctgtaa gcagttccag catctccgcg gcgcggctcc
     1381 ctccccgctc tttctcccga aggtagagat agattccgtc gcagtggttg tccacggtga
     1441 gaatttcggt ctccacgccc cgagcggcga agtcgagcgt cttttcggcg aggaaa
//
LOCUS       DAOSWW010000769         1494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_76094_length_1494_cov_3.433634, whole genome shotgun sequence.
ACCESSION   DAOSWW010000769 DAOSWW010000000
VERSION     DAOSWW010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76094_length_1494_cov_3.433634"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PK442_12860"
     CDS             <1..441
                     /locus_tag="PK442_12860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOO88587.1"
                     /translation="RSDAFSSIGTMVGIGGAIFLGRNWAFLDPLASLIVSVFIFKVSF
                     EILMPAVNELLESSLQDEEIAYIKKIIEASPHIQNYHHLRTRRIGAKVAIEVHFVFEK
                     TMSIREAHSYATEVEKKIQEYFGASSLITTHLEPSRDAQEATRA"
     gene            636..>1494
                     /locus_tag="PK442_12865"
     CDS             636..>1494
                     /locus_tag="PK442_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOO88588.1"
                     /translation="MKRSLWNKIGLTLLALLVLAGAACAAGTNNEWGGAKAKYVFMFI
                     GDGMGLQQVNAAEVYNGSINRTGGEPAIKKLALSGLPVQGMITTYSTNSFITDSAPAA
                     TSLASGYKTDNGVIGMDPAKKQKFTTVAQMVRAKGMKVGVLSSVSLNHATPASFYASV
                     PSRNDYYGIGEQLLDSGFDYFAGGGIHQSTGKEKDRKDLYEVAKEKGYQVLRTREEIL
                     AAKPGTKVIAVNPVLDGSNAMPYDMDRTPEQLSLAEFTRKGIELLDNPNGFFMMVEGG
                     KIDWACHAND"
BASE COUNT          332 a          428 c          453 g          281 t
ORIGIN      
        1 cggtcggatg cgttttcctc cataggaacg atggtcggaa tcggaggagc gatattttta
       61 ggtcgaaact gggcctttct tgatcctctg gcgtctctga tcgtcagcgt gtttattttc
      121 aaggtgtctt tcgagatttt gatgccggcc gtcaatgaac tgctggagtc ctctcttcag
      181 gatgaagaga tcgcatacat caaaaaaatc atcgaagcct cgccgcatat tcaaaattat
      241 caccatctgc gaacgagaag gatcggggcg aaggttgcca ttgaagttca cttcgttttt
      301 gaaaaaacca tgtcgattcg cgaggcgcat tcctatgcaa ccgaagtgga aaaaaagata
      361 caagagtact tcggcgcttc cagtcttata acgacgcatc tggagccgag ccgcgacgcg
      421 caggaagcga cgcgggcata agcgggaagg ggctgcggag acggacgcgc cgtatacgga
      481 catctttccg gtgcgctctc cgcgggatac tgaggccgga ggaggcgcgg cgaccgcgcg
      541 ccgagcgagt tttaccctcc cgatacactt cttcgcttct tccgttaccc tccttgaaga
      601 taattggctc aaattcttcg aggagggtaa aaaatatgaa gcgttcactg tggaacaaga
      661 tcggattgac gctgctcgcg ctgctggtgc tggcaggggc ggcctgtgcg gcggggacga
      721 acaacgaatg gggcggcgcg aaggcgaagt acgtgttcat gttcatcggc gacggcatgg
      781 ggttgcagca ggtcaacgcg gcggaggtct acaacggctc catcaacagg acgggcggcg
      841 agcccgcgat caagaagctg gccctgagcg gccttccggt gcaggggatg atcaccacct
      901 actccaccaa ctccttcatc accgactcgg cccccgcggc gacctcgctg gcctccggct
      961 acaagacgga caacggcgtt atcggcatgg acccggccaa gaaacagaag ttcaccaccg
     1021 tggcccagat ggtccgcgcc aaggggatga aggtcggcgt gctcagctcc gtctcgctga
     1081 accacgcgac ccctgcgtcc ttctacgcct ccgttccttc acgcaacgac tactacggta
     1141 tcggcgagca gcttctcgac agcggcttcg actacttcgc gggcggcggc atccatcaga
     1201 gcaccggcaa ggagaaggac cgcaaagacc tctacgaggt cgcgaaggag aaggggtatc
     1261 aggtgctccg cacccgcgag gagattctcg cggcgaagcc gggaacgaag gtcatcgcgg
     1321 tgaaccccgt gctcgacggc agcaacgcca tgccctacga catggacagg acgccggagc
     1381 agctctccct cgccgagttc acgcgcaagg gcatcgagct gctggacaac ccgaacggat
     1441 tcttcatgat ggtcgagggc ggcaagatcg actgggcctg tcacgcgaac gacg
//
LOCUS       DAOSWW010000770         1492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_76246_length_1492_cov_3.273486, whole genome shotgun sequence.
ACCESSION   DAOSWW010000770 DAOSWW010000000
VERSION     DAOSWW010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76246_length_1492_cov_3.273486"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..822)
                     /locus_tag="PK442_12870"
     CDS             complement(64..822)
                     /locus_tag="PK442_12870"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOO88589.1"
                     /translation="MLVFGHRGASGYAPENTLPAFELGIRQGCEGFEFDVQLTKDGVP
                     VVHHDWTVDRTTTGSGAIPDLTFEEIRAFDAGKWFSEEFTGTTVPRLEELFELVPDTM
                     PLNVELKSRPHDRPGLEEAVAALLERYNRVENTIISSFNHKSLKTMRRLNPAFRLGIL
                     YEGVLLSPFSYAKRQDLDLYSLHPCHDYVSAELVEKAHARGIKVACWTVNTKERAREL
                     EAAGVDVIITNYPDRCLPEAPKGKKGKKKAGKQQ"
     gene            complement(890..>1492)
                     /locus_tag="PK442_12875"
     CDS             complement(890..>1492)
                     /locus_tag="PK442_12875"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88590.1"
                     /translation="IILGLRFGMFTPTEAGAIAVLYCSLVGFFVYRELRPVHFITILK
                     DTVYGTSTVLLIIVAASVFGYYMTWERIPQTVAALLLDIAHNKYTMLLVINIMLLILG
                     MFIEGGAAMIILAPLLVPVVVGLGVDPVHFGLVIIVNIMIGGVTPPFGSMMFTTCSIT
                     GVAIPDFIRESIPFIIALLVALLIVTYFPALTLFLPHLLY"
BASE COUNT          312 a          444 c          449 g          287 t
ORIGIN      
        1 ccgctcccat tcaagacgca cgcgtcctgc gctccgcgag agcgcaggac ggaggggctc
       61 aacctattgc tgcttacccg cctttttctt ccctttcttc cccttgggcg cctccggcag
      121 gcacctgtcg gggtagttgg tgatgatcac gtctaccccc gcggcctcca gctcgcgggc
      181 acgctccttc gtgttgaccg tccagcacgc gactttgatc ccgcgggcgt gcgccttctc
      241 caccagctcc gccgagacgt agtcgtggca ggggtgcagg ctgtagagat ccagatcctg
      301 gcgttttgcg taggagaacg gggagagcag aaccccttca tagagtatcc ccaggcgaaa
      361 cgcaggattc agcctgcgca tcgtcttcag gcttttatgg ttgaaggagg agatgatcgt
      421 gttctcaacc cggttgtacc gctccagtag tgcggcgacg gcttcttcaa gccctggccg
      481 gtcgtgcggt ctgctcttca gctccacgtt gagcggcatc gtatccggaa ccagctcgaa
      541 cagctcttcc aggcgcggaa cagtcgtccc cgtaaactcc tccgagaacc atttccccgc
      601 gtcgaacgca cgaatttctt cgaatgtgag atcgggtatg gcgccgctcc ccgtggtcgt
      661 gcggtccacg gtccagtcat gatgcacgac gggcacgccg tctttggtca gctgcacatc
      721 gaattcaaaa ccctcgcacc cctgccggat tccgagttca aaagccggca gagtattctc
      781 cggggcgtac cccgaggccc ccctgtgtcc gaagacaagc actgaaaccc cttctttcta
      841 taggtagaga gtatgtaagg agcgcgccga agcgcgctcc tcccgtcgtt cagtagagca
      901 gatgcggtaa aaagagcgtg agcgccggga aataggtcac gatcagcaac gctacgagca
      961 gtgcgatgat gaacgggatg ctctcacgta tgaagtccgg aatggcgacg ccggtgatcg
     1021 agcaggtggt gaacatcatc gacccgaacg ggggcgtcac gccgccgatc atgatgttca
     1081 cgatgatgac cagtccgaag tggaccggat cgacgccgag gccgacgacg accggcacca
     1141 ggagcggcgc caggatgatc atggccgcgc ccccctcgat aaacatcccg aggatgagga
     1201 gcatgatgtt gatcacgagg agcatcgtgt acttgttatg cgctatatcg agcagcaggg
     1261 cggcgacggt ttgcggtatg cgctcccagg tcatatagta gccgaagacc gacgcggcga
     1321 cgatgatcag aagcacggtg ctggtgccgt agacggtatc cttcagaatg gtgatgaaat
     1381 gaaccggccg aagctcgcga tagacgaaga atccgacaag cgagcagtag agcaccgcga
     1441 ttgcgccggc ctctgtaggg gtaaacatgc cgaagcgcag tccaagaatg at
//
LOCUS       DAOSWW010000771         1487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_76633_length_1487_cov_3.518855, whole genome shotgun sequence.
ACCESSION   DAOSWW010000771 DAOSWW010000000
VERSION     DAOSWW010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76633_length_1487_cov_3.518855"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK442_12880"
     CDS             <1..209
                     /locus_tag="PK442_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88591.1"
                     /translation="HDRLSGIEVGLDGRAPVVEFFAGGRVRFVPPYEQPLPEVVLRPS
                     FTPLSGGDNVLLRGERWTLLQRAR"
     gene            290..1393
                     /locus_tag="PK442_12885"
     CDS             290..1393
                     /locus_tag="PK442_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88592.1"
                     /translation="MAFPLWRRARCCFVLLLLLCMIFSGTAGAALLRDLESAVRLRSG
                     SGIPVRQSGDGYAVDAALFLLYAVESGNRALFDELLPPFRRAFIVPFEAGAHTSGTVG
                     WKRRGKEPPDASGTTETMLAAKALWRAGVLWKNESFLEESLSLIRGYLAHESGKEKSW
                     QIRNYYNYGTRAFAVNSYALGYFPDFLFEVARSGLLPEASEAAERSLRFLRNAALPNG
                     LFSSSFDPELRTLFPEAEFSYHFSPDFRIKPGDSAFIALEMAESDPTISRRTLALLKV
                     FWPSIPDTVYGFLSLELSRAERRPEISTGHLAGVYRLALRFDEAFAREILPVLNERAL
                     SLLSGNEEEAWTWDAVNMLLALLDGERPGELAR"
BASE COUNT          244 a          423 c          472 g          348 t
ORIGIN      
        1 tgcatgatcg gctctccggt atcgaggtcg gtctggacgg acgcgctccg gtggtggagt
       61 ttttcgccgg ggggagggta cgctttgtcc cgccgtatga gcagcctctt cctgaagttg
      121 ttctgcggcc ctcctttaca ccgctatccg gcggtgacaa cgtgctgctg cggggcgaac
      181 gatggactct tctgcaaagg gcgcgctgaa tgtgttcgca ggtgttttcc ctctccggcg
      241 gtacggtttc aaccttgagc cgcccttaga gcaggggagg ggggccgctg tggcgtttcc
      301 tttatggaga agagcgcgct gctgtttcgt tctcttgctg ctattgtgca tgattttctc
      361 cggaactgcc ggagctgctc ttctgcgcga tctggaatcg gcggtgcggc ttcggagcgg
      421 ctccggaatt cctgtgaggc aaagcggcga tgggtatgct gtggacgccg cgctctttct
      481 tctctatgcg gtcgaatcag gaaaccgcgc gctcttcgac gagctgcttc caccctttcg
      541 ccgggccttc atcgttccgt tcgaggcggg agcgcatacg agcggcacgg tcggatggaa
      601 gagaagaggg aaggagcctc ccgacgcctc gggaacgacc gagacgatgc tcgcggccaa
      661 ggcgctctgg cgcgccggcg ttttgtggaa gaatgaatcg ttcctcgaag agagcctttc
      721 gctgatacgg ggctatcttg cccacgagtc cggcaaggag aagagctggc agatccgaaa
      781 ctactacaac tacggtacgc gagccttcgc cgtgaacagc tacgctctcg ggtatttccc
      841 cgactttttg ttcgaagtgg cgaggagcgg gttgcttccg gaagcgtcgg aggccgcgga
      901 gcgttcgctt cgctttctcc ggaacgccgc tttaccgaac ggcctttttt ccagctcttt
      961 cgaccctgag ttacgcacgc tttttcccga ggccgagttt tcatatcact tttctcccga
     1021 cttccgcatc aaaccggggg attccgcgtt catcgcgttg gagatggcgg agagcgatcc
     1081 gactatttcc cgcagaacgc tcgcgctgtt gaaggttttc tggccctcta ttcccgatac
     1141 cgtttacggc tttctttctc ttgaactctc aagggcggag agacggccgg agatttccac
     1201 cggacacctg gcgggggtct accgtcttgc gcttcgcttc gacgaggcgt tcgcgcgcga
     1261 gatactgccg gtgctgaacg aacgcgcgct ttctctgctc tccgggaacg aagaagaagc
     1321 gtggacctgg gacgcggtga acatgctgct ggcgcttctt gacggggaac gtcccgggga
     1381 actggctcgg taagcgggag ccccccaacg tattgaacgc gatgcgagcg aaaagaacgc
     1441 gccgactccc cggtttcgga gagcggcgcg tttgttgttt ttgagac
//
LOCUS       DAOSWW010000772         1484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_76893_length_1484_cov_4.799860, whole genome shotgun sequence.
ACCESSION   DAOSWW010000772 DAOSWW010000000
VERSION     DAOSWW010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76893_length_1484_cov_4.799860"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PK442_12890"
     CDS             complement(<1..255)
                     /locus_tag="PK442_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOO88593.1"
                     /translation="MKRRVFLLLALVLVVSFSGTAFAAKEGWPDQLRFMAGPPGGNWF
                     ALGGALADMWSKEVLQTTSITGGGVANVVNTNNTKGDLGFS"
     gene            576..>1484
                     /locus_tag="PK442_12895"
     CDS             576..>1484
                     /locus_tag="PK442_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOO88594.1"
                     /translation="MQQLASYNGKLFDSPETLLEFLRLEEKASERMGKLYAYATMKSH
                     ENTSEALYQGMADRAGMLYSSFAAVCSFFNPEVLAAGEEKVRDFIARCTGLALYRHFF
                     DDLLRSKEHVLSPREEELLARSSEMARTAETAFSLFTNADMTFPSIVDEKGEETELSE
                     ERYYLLSRSRDRRVRKEAFEGLLGTYGKYRNSLGALYSGSVKGDIFYATARKYQSSLD
                     ASLDADNIPSSVYDNVVSTVNANLDAMHDYMRLRKKALALDELHMYDLNVPLADEPDA
                     AIPYGKAVEMVAEGLAPLGEEYIDVLR"
BASE COUNT          316 a          401 c          435 g          332 t
ORIGIN      
        1 tgaaaaccca agatcgccct tggtattgtt tgtattcacc acgttggcaa cgccgccgcc
       61 ggtgatgctg gtggtctgga ggacttcctt cgaccacata tcggcaagtg cgccgccgag
      121 ggcgaaccag ttgccgcccg gaggtccagc catgaagcgg agctgatcgg gccacccttc
      181 ctttgccgcg aaggccgttc ctgaaaagga gaccacgagt acgagggcga gaagcagaaa
      241 aacgcgtctt ttcatttttt acacactcct ttgatgagat tttgagtaga acttccctga
      301 gaaacagacg gaaaaatgat atagtcttcg gggcggcatg tcaataaaac cgtttgaaca
      361 ttatttttca aaatttcctt gtttgtgaaa ggagtttcgt tatgattcgt actcagtctg
      421 gcaatggagc aaaaaaggcc gcttttacag aaatatcttt cggaggaaag gtccctcctc
      481 gctgtgatat tcctgtcgag tatacttggg atctctctgc gcttcagcct tccgacgagg
      541 catgggagga gtctttccgt cgcgccggtg aggagatgca gcagcttgcc tcctacaatg
      601 ggaaactctt tgattctccc gagacgcttt tggaatttct gcgcctggag gagaaggcgt
      661 ccgaacgtat gggaaagctg tatgcctacg cgacgatgaa gagccacgag aatacctccg
      721 aggcgctcta tcaggggatg gcggacaggg ccgggatgct gtactcctcc ttcgccgccg
      781 tctgctcttt cttcaacccg gaagtccttg ccgccggaga ggagaaggtg cgcgacttca
      841 tcgcgcgttg caccggtctt gcgctctacc gccacttctt cgacgatctt ctgcgctcga
      901 aggaacatgt gctgtcgccg cgcgaagagg aacttctggc gcgttcttcc gaaatggccc
      961 gtacggcgga gacagcgttt tcgcttttta cgaacgccga tatgaccttc ccgtcgatcg
     1021 tcgacgagaa gggggaggaa acagagcttt cagaggagcg gtactacctt ctgagccgtt
     1081 cgcgggatcg ccgcgtgcgc aaagaggcgt tcgaaggtct gctggggacc tacgggaagt
     1141 accggaactc gctcggcgcg ctctattccg ggagtgtgaa gggggatatc ttctatgcga
     1201 cggcgcggaa gtatcagtcc tcgctggatg cttcgctcga cgcggacaac atcccctcct
     1261 cggtctacga caacgtcgtt tcgacggtga acgccaacct ggatgcgatg cacgactaca
     1321 tgcgtctcag gaagaaggcg ctcgccctgg acgagctgca catgtacgac ctcaacgtac
     1381 cgcttgcgga cgaacccgac gcggccatac cgtacgggaa ggcggtcgaa atggtggccg
     1441 aggggcttgc ccctctcggg gaggagtata ttgacgtcct ccga
//
LOCUS       DAOSWW010000773         1484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_76960_length_1484_cov_1.923723, whole genome shotgun sequence.
ACCESSION   DAOSWW010000773 DAOSWW010000000
VERSION     DAOSWW010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76960_length_1484_cov_1.923723"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="PK442_12900"
     CDS             <1..138
                     /locus_tag="PK442_12900"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOO88595.1"
                     /translation="PSVELVLTGRYAWEELMERADLVTEMQEIRHYFAKGVPARRGIE
                     K"
     gene            135..587
                     /locus_tag="PK442_12905"
     CDS             135..587
                     /locus_tag="PK442_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88596.1"
                     /translation="MTHPRIVIAGTNSGSGKTTFTAGLISALRSRGMTVQPFKTGPDY
                     IDPGFHTLAAGRPCRNLDTMLLSRDGTLELFDRNSSGADISLVEGVMGLFDGAARATN
                     GEAPHTWRRCSKPLCSLWSTAARWRGAPRRSSSVSRSSTGASRSPECF"
     gene            491..>1484
                     /locus_tag="PK442_12910"
     CDS             491..>1484
                     /locus_tag="PK442_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOO88597.1"
                     /translation="MVNGRSMARSAAALVLGFSKFDRRVAVAGVLLNALGSANHYRIL
                     KEAIEGDTGIPVFGYLPRTADIAVPERHLGLVPSVEQEQGAEMIESIRTLVEEHVDVG
                     ALLELARRAPLLPSYSPAIFAPPATPRGARIAVARDEAFHFYYQDNLDILEHLGATLL
                     PFSPLRDPKLPEGTQGVYIGGGFPEEFAEKLSANESLRAELRAAADRGMPLFAECGGL
                     MYLVERLEDKSGTAHRMAGVFPGVTRMGKRLQALGYCGGTLERGALIGRKGASLRGHL
                     FHWSIYDSEGNDELFSLRLEKNGSIYRDGLAKKNAFASYLHLHFGTNLAPARRFL"
BASE COUNT          261 a          497 c          485 g          241 t
ORIGIN      
        1 ccctcggtcg aactggtgct caccgggcgc tacgcctggg aggagctcat ggagcgcgcc
       61 gaccttgtca ccgagatgca agagatccgc cactacttcg cgaagggcgt tcccgcccgg
      121 agaggaatcg aaaaatgaca catccgcgta tcgtgatcgc agggaccaac agcggttccg
      181 ggaagacgac ctttacggcc gggctgatct ccgcgctccg ttcgcgcggg atgaccgtcc
      241 aacccttcaa gacggggccg gactatatcg accccggctt ccacaccctg gccgcggggc
      301 ggccctgccg gaacctcgac acgatgctgc tctcgcgcga cggcacgctg gagctgttcg
      361 accgcaactc ctccggcgcg gacatctcgc tggtcgaagg cgtgatggga ctcttcgacg
      421 gcgcggcgcg cgcgacgaac ggggaagcac cgcacacctg gcgaagatgc tcaaagcccc
      481 tgtgctcctt gtggtcaacg gccgctcgat ggcgaggagc gccgcggcgc tcgtcctcgg
      541 tttctcgaag ttcgacaggc gcgtcgcggt cgccggagtg cttctgaacg cgcttggaag
      601 cgccaaccac taccggattc tcaaggaggc catcgagggc gatacgggca tcccggtctt
      661 cggctacctg ccgcgcactg ccgatatcgc cgtgccggag cgtcacctcg ggcttgttcc
      721 ctcagtcgaa caggagcagg gcgcggagat gatcgaaagt atccgcaccc tcgtcgagga
      781 gcatgtcgac gtcggggcgc tcctcgaact cgcccggcgc gccccgctcc ttccgtcgta
      841 ctcccccgcg atcttcgccc ccccggcgac gccgagaggc gcaaggatcg cggtcgcgcg
      901 cgacgaggcc tttcacttct actatcagga caacctggac atccttgagc atctgggagc
      961 gacgctgctc ccattcagcc cgctgcgcga cccgaagctg cccgaaggga cgcagggggt
     1021 gtacatcggc ggcggctttc cggaggagtt cgcggagaag ctttccgcca acgaatcgct
     1081 ccgcgcggag ctgcgagcgg cggcggaccg cgggatgccc ctcttcgcgg agtgcggcgg
     1141 tctgatgtac cttgtcgaac ggcttgaaga caaatcgggg acggcgcacc gaatggcagg
     1201 ggtctttccg ggagtcaccc ggatgggcaa gcgcctgcag gcgctcgggt actgcggagg
     1261 gacgctcgaa cgcggcgcgc tgatcggaag gaagggcgcg tcgcttcgcg gacatctctt
     1321 ccactggtcc atctacgaca gcgaagggaa cgacgagctc ttctccctcc ggctggagaa
     1381 aaacgggagt atctaccgcg acgggctggc gaaaaagaac gccttcgcaa gctacctcca
     1441 tctgcacttc ggaacgaacc tcgccccggc gcggcgcttt ctgg
//
LOCUS       DAOSWW010000774         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_76987_length_1483_cov_4.870448, whole genome shotgun sequence.
ACCESSION   DAOSWW010000774 DAOSWW010000000
VERSION     DAOSWW010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76987_length_1483_cov_4.870448"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PK442_12915"
     CDS             <1..562
                     /locus_tag="PK442_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOO88598.1"
                     /translation="FSMTAKKLEMPAYDGRAVQGIGPNYATSNRGGCHVRGYTTSPEV
                     LGLPQKLDPAEYQSKPTWVKIFQDLTAAIDSSGMCLFTSFALGADDYAAFLKAATGFE
                     YDGAEALKAGERIWNIERLFNLREGMDTAEDTLPARLLNEPIPEGPAKGMVSHLPEML
                     PEYYKLRGWDSKGIPTTGKLEELGLQ"
     gene            567..839
                     /locus_tag="PK442_12920"
     CDS             567..839
                     /locus_tag="PK442_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOO88599.1"
                     /translation="MIKVLFFATIRDYTNEKETTAEGASSVEGLLRLLCARYGPEFEA
                     EVFDGGKISDRVIVLVNGKHIAHTGGPLTPVKDGDTIAVFPIIGGG"
     gene            872..>1483
                     /locus_tag="PK442_12925"
     CDS             872..>1483
                     /locus_tag="PK442_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOO88600.1"
                     /translation="MKRTVELTDLLRYHFLSAPTFSPDGTKIAFLLHKADYENNAYSS
                     NIWMHDLLTGSLRQLTASGKEKFFAWKHDNRTLVFASGRNDAPKTETVFYTLDICGGE
                     AVPLRTVKRALSSFTVLSDGTFLALGVFEQAFDNPYDAEMLFFDQVPFCSNGKGFTGQ
                     KKKSLCLYTPEGEEKRLTPEDMDVENYVVSPDGAKLLAWGPVFK"
BASE COUNT          329 a          478 c          413 g          263 t
ORIGIN      
        1 gttctccatg acggccaaga agctggagat gcccgcgtac gacggacgcg ccgttcaggg
       61 gatcggcccg aactatgcca cctccaaccg cggcggctgc cacgtccgcg gatacacgac
      121 ctcccctgaa gtgctcggcc tgcctcagaa gctcgacccg gcggagtatc agtcaaagcc
      181 gacgtgggtc aagatcttcc aggatctcac cgcggcgatc gactcctccg gcatgtgcct
      241 cttcacctcc ttcgccctgg gcgccgacga ctacgccgcg ttcctgaagg ccgccaccgg
      301 cttcgaatac gacggcgccg aagccctgaa ggcaggcgag cgcatctgga atatcgagcg
      361 tctgttcaac cttcgcgagg gtatggatac agcagaggac acgctccccg cgcgccttct
      421 gaacgaaccg atccctgaag gcccggccaa gggcatggtg agccacctgc cggaaatgct
      481 tccggaatac tacaagcttc gcggctggga ctccaaaggc atccccacca ctggaaaact
      541 ggaggaactc gggttacaat aagcgcatga taaaagtact gttcttcgcg actatccggg
      601 actacacgaa cgagaaggag acaacggcgg agggagcctc ttcagtagag gggctcctcc
      661 gtctcctttg cgcgcgctac ggtcccgagt tcgaagccga ggttttcgat gggggaaaaa
      721 tttccgaccg ggtgatcgta ctggtgaacg gcaaacacat cgcccacacc ggaggaccgc
      781 tcaccccggt caaggacggc gacacgatcg ccgtcttccc tatcatcggc ggaggatagc
      841 cgcacacttc caggcaaagg ggaccagaaa attgaaaaga accgtagaac tcaccgatct
      901 gcttcgatac cactttctct ccgcaccgac gttctctccc gacggcacga aaatagcatt
      961 tctcctccac aaggccgact acgaaaacaa cgcctacagc tcgaacatct ggatgcacga
     1021 cctgcttacg ggaagccttc ggcagctcac agcctccggc aaggagaagt ttttcgcgtg
     1081 gaagcacgac aacaggacgc tcgtcttcgc cagcggacgc aacgacgccc ccaagacgga
     1141 gacggtgttc tacacgctcg acatctgcgg aggcgaggcc gttccgcttc gcaccgtgaa
     1201 gcgcgcgctc tcctcgttta ccgtcctttc ggacggcact ttccttgcgc tcggcgtctt
     1261 cgagcaggcg ttcgacaacc cttacgacgc ggaaatgctc ttcttcgacc aggtaccttt
     1321 ctgctccaac ggaaaggggt tcacggggca gaagaaaaag tcgctctgcc tctacactcc
     1381 ggagggcgag gagaaacgcc tcacccccga ggatatggac gtggaaaatt acgtcgtctc
     1441 tccggacggg gcgaaactgc tcgcttgggg tcccgtcttc aag
//
LOCUS       DAOSWW010000775         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77032_length_1483_cov_2.548319, whole genome shotgun sequence.
ACCESSION   DAOSWW010000775 DAOSWW010000000
VERSION     DAOSWW010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77032_length_1483_cov_2.548319"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PK442_12930"
     CDS             <1..402
                     /locus_tag="PK442_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO88601.1"
                     /translation="VLELMRSLKDEFSTAMIMITHDLGVVGEVCDHIAVMYAGRIVET
                     GTLEQLFRSAKHPYTTGLFRCIPDIEEETSLIQPIEGLMPEPLDLPKGCAFHPRCPRC
                     MDVCRTTLPPASEENGHRVLCHLYAGGGAHA"
     gene            395..1345
                     /locus_tag="PK442_12935"
     CDS             395..1345
                     /locus_tag="PK442_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88602.1"
                     /translation="MPDLVKVENLVKYFPVAAGNVHAVDDVSFAIKEGETLGLVGESG
                     CGKSTTGRLLIRLIEATSGRILYRGRDIASMDKKELHGIRKQVQIIFQDPFSSLDPRK
                     SIGETIGKPLEIFGMGSKREREEMVDALMEKVGLSPRLYYKYPHELDGGRRQRVGIAR
                     ALSLSPDFIVCDEPVSALDVSIQAQILNLLQELQEKMKLTYLFVSHDLSVVKHISNRI
                     AVMYLGKIVESAPSLELFKNTLHPYSQALLAAVPIPKLDRKREKFILTGGVPSPINPP
                     PGCRFHTRCPWKMDVCSAEEPPLIDVGGEHLVACHKVKGS"
BASE COUNT          303 a          466 c          470 g          244 t
ORIGIN      
        1 gtgctcgaac tgatgcgaag cctgaaggac gagttctcga cggcgatgat catgatcacg
       61 cacgacctcg gcgtggtggg cgaagtctgc gaccacatcg cggtgatgta cgccggacgg
      121 atcgtcgaga ccggcacgct ggagcagctc ttcagaagcg cgaagcaccc gtacaccacg
      181 gggctcttcc gctgcatccc cgacatcgag gaggaaacgt ccctcatcca gcccatcgaa
      241 gggctgatgc cggagccgct cgacctgccc aaggggtgcg cgttccaccc ccgctgcccg
      301 cgctgcatgg acgtctgccg gacgaccctt ccgccggcca gcgaggagaa cgggcacagg
      361 gtgctgtgcc atctgtacgc cggaggtgga gcccatgcct gatctcgtga aggttgaaaa
      421 tctggtcaag tacttccccg tcgcggcggg aaacgtccac gcggtggacg acgtctcctt
      481 cgcaatcaag gagggggaga cgctcggcct cgtcggcgag tcggggtgcg gcaaatccac
      541 caccggacgg ctgctgatcc gcctcatcga ggccacctcg gggcggattc tgtaccgggg
      601 acgggacatc gcctccatgg acaaaaaaga actgcacggt atccgcaagc aggtgcagat
      661 catcttccag gacccgttct cctcgctcga cccgcgcaag agcatcggcg agaccatcgg
      721 caagccgctg gagatcttcg gtatggggtc gaagcgcgag cgggaggaga tggtggacgc
      781 cctgatggag aaggtgggct taagcccccg cctctactac aagtacccgc acgagctgga
      841 cggcggacga aggcagcggg tcggcattgc ccgcgcactc tcgctctcgc cggacttcat
      901 cgtctgcgac gagccggtct cggcgctcga cgtctccatt caagcgcaga ttctgaacct
      961 gctccaggag cttcaggaaa aaatgaagct gacctacctc ttcgtgtcgc acgacctctc
     1021 ggtggtgaag cacatcagca accgcatcgc cgtgatgtac ctggggaaga ttgtcgagtc
     1081 ggcgccctcg ctggagctgt tcaaaaacac cttacacccc tactcgcagg cgctgctcgc
     1141 ggcggttccg atcccgaagc tcgaccggaa gcgggagaag ttcatcctta ccggaggcgt
     1201 ccccagtccg atcaacccgc cgccggggtg ccgcttccac acgcgctgcc cgtggaagat
     1261 ggacgtctgc tccgccgagg agccgccgtt gatcgacgtg ggcggcgaac acctggtggc
     1321 ctgtcacaaa gtgaagggct cgtgatatca tagaggcaga gtgctcgccg cgacttcttt
     1381 acaccggtga gaacagctgg ggaaacacgc agcaggataa tctttcgggg cgcgacaacc
     1441 gagaaataaa ggaaacgcat cccccgagaa ggaggtgagg cgc
//
LOCUS       DAOSWW010000776         1482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77090_length_1482_cov_3.375613, whole genome shotgun sequence.
ACCESSION   DAOSWW010000776 DAOSWW010000000
VERSION     DAOSWW010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77090_length_1482_cov_3.375613"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK442_12940"
     CDS             complement(<1..343)
                     /locus_tag="PK442_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOO88603.1"
                     /translation="MYFIEGQSHDPAYNLALEEHLYRSVNEGHPGYFLLWQNAPSIIV
                     GRFQNTAQEVNRPFVEERGIHVVRRISGGGAVYHDLGNVNYSFIVPNDEGTPFDFARH
                     ARPVIDALAKLG"
     gene            436..1305
                     /locus_tag="PK442_12945"
     CDS             436..1305
                     /locus_tag="PK442_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO88604.1"
                     /translation="MLDSLEIITLMEDSVPMNGHLVAEHGLSFFIKGTKDGKEVRGLL
                     DVGQTAEFLAHNMKELGVCPQSVDFVVLSHCHYDHTGGIAKFVESTGKHDFPVIANPA
                     LFRPHFVMEPVVAAKGVRTGDEREAIERAGGMLLLSGAPFPLADGLSTTGEIPRETDF
                     EGPGKRFFTVRDGEVVQDSLPDDMGIVARVKGKGLVVLAGCSHSGIVNILKRVRALYP
                     GEPIEGVVGGLHLVNGTEEKMEKTLAGLKEFDPAWIACGHCTGFPMQAKLFQAFGKRF
                     IPLTVGKRFIVGA"
BASE COUNT          272 a          418 c          477 g          315 t
ORIGIN      
        1 cgccgagttt tgcgagcgcg tcgatcactg gccgggcatg gcgcgcaaag tcgaaggggg
       61 ttccctcgtc gttggggacg atgaacgagt agttcacgtt cccgaggtcg tgatagaccg
      121 cgcctcctcc ggatatccgt ctgacgacgt gaatgccgcg ctcctcgacg aaggggcggt
      181 tcacctcctg ggccgtgttc tggaagcgcc ccacgatgat cgagggagcg ttttgccaca
      241 gcaggaagta gccggggtgt ccttcgttga cgctcctgta gagatgctcc tcgagcgcga
      301 ggttgtacgc ggggtcgtgc gattgtcctt cgataaagta catttcgatt tctctcctct
      361 cgttccggcg cctcttgcaa agtcggcggt ttcagttcat tatacaaaat atcctgtatc
      421 cggaggtgga gcgatatgct cgattcactt gagattatca cattgatgga agactcagtg
      481 cctatgaacg gtcaccttgt cgccgagcac ggcctttcgt tctttattaa ggggacgaaa
      541 gacggaaagg aggtccgagg tctcctcgac gtggggcaga ctgcggagtt cctggcgcac
      601 aacatgaaag agcttggggt ttgcccgcag tcggtcgact ttgttgttct ctcacactgt
      661 cactacgacc acaccggagg tatcgcgaag ttcgttgagt ccacagggaa gcatgacttc
      721 ccggtgatcg cgaatcctgc gctctttcgt ccgcactttg taatggaacc ggttgtggcg
      781 gcgaagggcg tccgtaccgg cgacgagcgc gaagctatcg agagggccgg cggaatgctg
      841 ttgctctcag gcgcgccgtt tcctcttgcc gacggactgt cgaccactgg agagattccg
      901 cgcgagaccg atttcgaggg gccggggaag cgtttcttca cggtacgcga cggcgaggtg
      961 gtccaggact ccctccccga cgacatgggg attgtcgccc gcgtcaaagg gaaggggctc
     1021 gtcgtcctcg ccgggtgcag ccattccggg attgtgaaca tcctgaagcg ggtccgcgcg
     1081 ctctaccccg gcgagccgat cgaaggcgtc gtcggcggac tgcacctcgt gaacggcact
     1141 gaagaaaaga tggagaagac gctcgcaggg ttgaaagagt tcgatccggc atggatcgcc
     1201 tgcgggcact gcaccggttt tcccatgcag gcgaagctct tccaggcgtt cgggaaacgc
     1261 tttattccgc tcacggtggg gaagcgcttc atcgtcggag cgtaagcggc gagcagctcc
     1321 cggacgacag aggggacggg gcgccagctt ccctgcatcc gtagagaata cgacgctctg
     1381 tcctcttatg gagcggcctc tcagtcgcct gcaagcgagc ggagtttgcg gctccgttcg
     1441 gcatctcccg ccgcttcggc gatgcgcgcc gaatagtcga ga
//
LOCUS       DAOSWW010000777         1480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77274_length_1480_cov_3.148772, whole genome shotgun sequence.
ACCESSION   DAOSWW010000777 DAOSWW010000000
VERSION     DAOSWW010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77274_length_1480_cov_3.148772"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1480
                     /locus_tag="PK442_12950"
     CDS             <1..>1480
                     /locus_tag="PK442_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOO88605.1"
                     /translation="AGVDGRVTEVRVMEGKRKVIITSENGDGEEKVTYNIPINQNLLV
                     NEGDSVLKSDKLTEGNFDPQQLLEVEGIEAVQRALVDEIQEVYRSQGVSISNKHIEVI
                     LRKVAPVNRVKINEEGDTSFVAGDLVWTDDIDAETAEIGRENERNIDEAVRIFTGIPL
                     KDVSAQKMNEAVLQHLGKPLDEEAIRTILRPGMLISSAILTDTEGKQFNLVVGEAAFR
                     KQLEGLELIGAFQSADGRGIEAGTRLTLGQLAIVTSNDPTPLLVRDEALLEKLVDTAY
                     IAEDIVADGESVAKADRVFTLEDAALCRERGVKSVMVWHSVERVNLLDILEESLKEKW
                     GKSLTRAIDIEGNAIADLPQLVDERVIKGILEGRIGAVEIEGAVFSRDHFMKELLSAR
                     VYGKILLEPVVDARGNVLAEAAQEVNQTVIERLSGSAGPTDASGNGIKELVVRPMGGH
                     KKEEKKIIQRVTFVRKLREGPKVKPFVHGITKAALATDSFLSA"
BASE COUNT          349 a          412 c          484 g          235 t
ORIGIN      
        1 cgcgggcgtg gacgggcgcg tgaccgaagt ccgcgtcatg gaggggaaac gcaaggttat
       61 catcacctcc gaaaacgggg acggcgagga aaaagtcacg tacaacatcc ccatcaacca
      121 gaatctcctg gtgaacgaag gggacagcgt gctcaagagc gacaagctca ccgaggggaa
      181 cttcgacccg cagcagcttc tcgaggtcga gggaatcgag gcggtgcagc gcgcgctcgt
      241 ggacgaaata caggaagtct accgctcgca gggcgtctcg atcagcaaca agcacatcga
      301 ggtcattctg cggaaggtgg ccccggtgaa ccgcgtgaag atcaacgagg agggcgacac
      361 atccttcgtc gccggggatc ttgtctggac ggacgacatc gacgccgaga cggcggagat
      421 cggccgcgag aacgagcgga acatcgacga ggcggtgcgc atcttcacgg ggataccgtt
      481 gaaggacgtg agcgcccaga agatgaatga agccgttctg cagcaccttg gcaagccgct
      541 cgacgaagag gcgatccgga cgatactccg ccccggaatg ctgatctcct ccgccatatt
      601 gacggatacg gaaggaaagc agttcaacct cgtggtcggc gaagccgcct tccggaagca
      661 gcttgagggg ctcgaactca tcggagcctt ccagtccgcg gacgggagag ggatagaggc
      721 aggaactcgg ctgacgctcg gtcagctcgc catcgtcacc tccaatgatc cgacgcccct
      781 gctggtgcgg gacgaggcgc tgcttgaaaa gctggtcgac accgcataca tcgccgagga
      841 cattgtcgcg gatggagagt ccgtggcgaa agccgacaga gtctttacgc tggaggacgc
      901 cgctctttgc agagagcgcg gcgtgaagag cgtgatggta tggcactcgg tggaacgggt
      961 caatcttctt gatatccttg aggaaagcct gaaggagaag tggggcaagt cgctcacgcg
     1021 cgccatcgac attgaaggga acgctatcgc cgatcttccg cagcttgtcg acgagagagt
     1081 aatcaagggg attctcgaag ggcgtatcgg ggccgttgaa atagagggcg ctgtcttctc
     1141 acgggatcac ttcatgaaag aactcctctc cgccagagtc tacggaaaga tactccttga
     1201 gcctgtcgtc gacgcccgag gaaatgtgct cgcggaggcg gcgcaggagg tcaatcagac
     1261 ggtcatcgaa agactctccg gcagcgccgg cccgacggat gcctccggaa acggtatcaa
     1321 ggagcttgtc gtccgcccga tgggagggca caagaaagaa gaaaagaaga tcatccagcg
     1381 ggtcaccttc gtccggaaac tgcgcgaagg cccgaaggtg aagcccttcg tccacggcat
     1441 cacgaaggcg gcgctggcca ccgacagctt cctcagcgcg
//
LOCUS       DAOSWW010000778         1479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77357_length_1479_cov_3.473315, whole genome shotgun sequence.
ACCESSION   DAOSWW010000778 DAOSWW010000000
VERSION     DAOSWW010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77357_length_1479_cov_3.473315"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK442_12955"
     CDS             complement(<1..341)
                     /locus_tag="PK442_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOO88606.1"
                     /translation="MLCIVPTPVGNLEDMTPRGVRMLAEADFIACEDTRTSMPLLRRY
                     GISKPLIAYHAFNEKGKTEALVERLLRGEKGALISDAGTPGISDPGYELIRRCIDEGV
                     EMTALPGPTAFV"
     gene            complement(329..1111)
                     /locus_tag="PK442_12960"
     CDS             complement(329..1111)
                     /locus_tag="PK442_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO88607.1"
                     /translation="MSAPENATHPVSRDELLMGSLSLDQPEKGPRVNADTILLASYVR
                     NTFSGARGGSDVLEIGCATGAVSLILAYRFPEIPRIVGVDIQGRLVQLAAANAVRNGF
                     ADRVSFIEADVRAARSSFPAQSFDVVAANPPYESPGRGRLSATHSERLARQEVSCTLE
                     EIAAACRYLLRNRGRLYLVFKAERACELFARLSEQGIEPKRVRWVHPLPGRKASVVLV
                     EALRGGRPGMIVEPPLFIHDATGGYTEEFLSAYTKEGLPCCV"
     gene            complement(1108..>1479)
                     /locus_tag="PK442_12965"
     CDS             complement(1108..>1479)
                     /locus_tag="PK442_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88608.1"
                     /translation="YLFASMEVAGVPLAVLDEVDAALDESNLLRFGELAREYACPSEE
                     ASNADAEDAGRAASSSAIQLIVMTHRRATMERADILYGVTLAEPGLSKVVGIRVEDWV
                     EPGTDPRGRRAPTAVSLRGRP"
BASE COUNT          261 a          495 c          477 g          246 t
ORIGIN      
        1 acgaaggcag tcggaccggg gagcgccgtc atctcgaccc cttcatcgat gcacctgcgg
       61 atgagttcgt acccggggtc ggagatccct ggcgtccccg cgtcggagat aagagccccc
      121 ttctctccgc ggaggaggcg ttcgacgagc gcctccgtct tccccttctc gttgaaggca
      181 tggtacgcga tgaggggctt cgagatgccg tagcggcgca gcagcggcat cgaggttcgc
      241 gtatcctcgc aggcgatgaa gtccgcctcg gcgagcatac gaacgcccct cggcgtcatg
      301 tcctcaaggt ttcccaccgg ggtggggact atacacagca cggcaatccc tccttcgtgt
      361 aggcgctcag aaactcctcc gtatatccgc ccgtggcgtc gtgaataaag agcggcggct
      421 cgacgatcat gcccggacgc ccgccccgga gcgcctcgac aagcaccacg gaggcttttc
      481 ttccggggag tggatggacc cagcgcactc gtttcggctc gatcccctgt tcggagagac
      541 gggcaaaaag ttcacaggcc cgctccgcct tgaagacgag gtagagcctc ccccggttcc
      601 tgagaaggta acggcaggcc gcggcgatct cctcaagcgt gcacgagacc tcctgcctcg
      661 caagccgttc ggagtgcgtc gcggagaggc gccctctccc ggggctctcg tacggcgggt
      721 tcgccgccac aacgtcgaac gactgcgcgg ggaaggacga gcgggcggcc cggacgtctg
      781 cctcgatgaa ggaaactctg tccgcgaagc cgtttctgac cgcgtttgct gcggcaagtt
      841 ggacgagtct cccctggatg tccaccccga cgatccgggg aatttccggg aagcggtacg
      901 caagaatgag agagacggcc cccgtggcgc agccgatttc aaggacgtcg cttcctccgc
      961 gcgcccctga gaacgtattg cgcacgtagg atgcgaggag aatggtgtct gcattgactc
     1021 tcggcccctt ctccggctgg tccagcgaga gcgagcccat caggagctca tcccgcgaaa
     1081 ccggatgggt tgcgttttca ggagcgctca cggccgcccc cggagggaaa cagccgtagg
     1141 cgcgcggcgt ccgcgaggat ccgtccccgg ctccacccag tcctcgacgc gtatgccgac
     1201 cactttcgag agtcccggct ccgcgagcgt cacgccgtag agaatgtcgg cccgctccat
     1261 ggtcgccctc cggtgcgtca tcacgatgag ctgaatcgcg gaggatgagg ccgcacggcc
     1321 ggcgtcctcc gcgtcggcgt tcgacgcctc ctccgaagga caggcgtact cccgggccag
     1381 ctcgccgaag cgcagcaggt tggactcgtc gagcgccgcg tccacttcgt caagaacggc
     1441 aagaggaacc cccgcgacct ccatggaggc gaagaggta
//
LOCUS       DAOSWW010000779         1479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77362_length_1479_cov_3.219101, whole genome shotgun sequence.
ACCESSION   DAOSWW010000779 DAOSWW010000000
VERSION     DAOSWW010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77362_length_1479_cov_3.219101"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="PK442_12970"
     CDS             <1..1367
                     /locus_tag="PK442_12970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO88609.1"
                     /translation="VWATLPTLELVKVLWYDSAHLMKEEADWRTKKNSRKGLDPVEPI
                     YTRDDVDDAIKMLSAASYDDKIPVAPGISVRFRDAGHILGSAILEVWLEEEGKQVKVV
                     FSGDLGPQQTVMERNPAIITSADYVIMESTYGDRLHKTNAESRDEFRDVLITALKDNA
                     KVFIPSFVVDRAQRILYELALLKDEGILAGNVPIFFDSPMGVKATEIYKKHMNLLSSE
                     IQQYVSQGKDPFAPGKLKYVSSVEESQAINDVRNAVVVAGSGMANGGRIVHHLKHGIW
                     NPKNHVVFVGYQAQGTLGRRIVEGEKNLRIAGEDVSVKANIHTINGFSAHADRDDLLA
                     WAGNFSTAPLFLITHGEPKSSLAFSETLAAAGMKGVVPVAGQEIELEPNGAGKAVHIA
                     EQPVVLKREEVSTLLAEILLLASGLQNRAVPVQEEGILSLLRSSKVLLETANEKMNER
                     LRAE"
BASE COUNT          383 a          354 c          410 g          332 t
ORIGIN      
        1 aagtatgggc tacgctgcct acactggagc ttgtgaaagt cctttggtac gactccgcac
       61 accttatgaa ggaagaggcg gactggcgga cgaagaaaaa ctccagaaaa ggactcgacc
      121 ctgttgagcc tatatacacc cgtgacgacg ttgacgacgc aatcaagatg ctctcagccg
      181 caagttacga tgacaagatc cctgttgccc ccggaatatc agtgagattc agagatgccg
      241 gccatatcct tggaagcgcc attctcgaag tctggctgga ggaagagggg aaacaggtca
      301 aggttgtctt cagcggcgat cttgggcccc agcaaactgt catggaaaga aacccggcta
      361 ttattacatc ggccgactac gtgatcatgg aatccacgta cggagatcgc ttgcacaaga
      421 ccaacgccga gagtagggac gaattccgcg acgttcttat tacagctctc aaagacaacg
      481 caaaggtttt tatcccgagc ttcgttgtgg accgggcgca gagaatcctc tacgaactgg
      541 ctctgttaaa ggacgaagga attctggccg gcaatgtccc cattttcttt gactctccga
      601 tgggcgtcaa agccacggaa atctacaaga aacacatgaa tcttctttcg tcggaaatcc
      661 agcagtacgt aagtcaaggg aaagacccgt ttgctccggg taaactgaag tacgtatcga
      721 gcgttgaaga atctcaggcg atcaatgatg tcagaaatgc cgtggtggtc gccggaagcg
      781 ggatggcaaa cggagggcga atcgtccacc atctgaagca cgggatctgg aatccaaaga
      841 atcatgtcgt ttttgtcggc taccaggctc aggggaccct tggccgcaga attgtagaag
      901 gagagaagaa tctacggatt gctggagaag atgtctcagt aaaagcgaat attcatacga
      961 taaacggttt ttctgcgcat gcggatcgtg acgatctgct tgcatgggca ggtaattttt
     1021 cgacggcgcc cctcttcctg attactcacg gcgaaccgaa atcttctctt gcgttttcag
     1081 aaacccttgc agcggcaggc atgaagggcg ttgttcctgt tgccgggcag gagatagaac
     1141 tcgaacctaa cggggcgggg aaagcggtcc acatagctga acagccggtg gtccttaaaa
     1201 gagaagaggt ttcaacgctg ctcgccgaaa ttcttctcct tgcatccggg cttcaaaatc
     1261 gtgccgtccc tgttcaggaa gagggaattc tctcgcttct gaggtcaagc aaggtgcttc
     1321 tcgaaacggc gaacgaaaaa atgaacgagc ggttacgagc ggaatagact gtcgcaggct
     1381 attttattcc gtatccatgt gggcacggag gtggaggtat gcgtaaaggt ggagtatccc
     1441 ccggagttct tatcgccggt ctcgtgctgt tcttgatta
//
LOCUS       DAOSWW010000780         1479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77367_length_1479_cov_2.909410, whole genome shotgun sequence.
ACCESSION   DAOSWW010000780 DAOSWW010000000
VERSION     DAOSWW010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77367_length_1479_cov_2.909410"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PK442_12975"
     CDS             <1..973
                     /locus_tag="PK442_12975"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOO88610.1"
                     /translation="RDGEDALRKIEELSPAVVTLDVEMPKKDGLATLEEIMKRRPLPV
                     IMVSSLTQEGAQITLRALSAGAVDFVAKPSGHVSLNMRDVSADLITKVLAASTAKIVV
                     PRERREPRAQPVAPTAARPMESFASRWRKGRPEMVAIASSTGGPRALQQVLSQIPGDF
                     PVPIVIVQHMPKDFTLSFAKRLDSLSELDVVEGAEGTELRPGLAVVAPGGYHMVVRKK
                     SPGKFACALSDAPPLLSVKPSANIMFLSVADEIGGNTVGVILTGMGRDGADGAAALKM
                     KGARIIAESQETCVVYGMPKAAVEQGVVDELLPLYSIPEAMIRSVRE"
     gene            1013..>1479
                     /locus_tag="PK442_12980"
     CDS             1013..>1479
                     /locus_tag="PK442_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOO88611.1"
                     /translation="MTNMDMSQYMGAFLDEAGDNLKHLDDLTLVIEKDPGNSEAIAEI
                     FRSAHTLKGMSATMGFDRMASLTHAMEDMLDAVRKGEYSLSAGDIDLLFRSLDTLQSM
                     VDAIRSVGSDASVDTDDLVKSIRAAGANRGAEPPAAEEKTLNDELSGQEQKWVA"
BASE COUNT          325 a          384 c          464 g          306 t
ORIGIN      
        1 tcgggacgga gaagacgctc ttcgcaagat tgaagagctt tctccggcgg ttgttacgct
       61 tgatgtcgag atgcccaaaa aagacggttt ggctactctt gaggagatta tgaagcgccg
      121 tcctcttcct gtgatcatgg tgagcagtct gacgcaggaa ggcgcgcaga ttacgctcag
      181 ggcgctttcg gccggagcgg tggattttgt cgccaagcct tcggggcatg tttcgctgaa
      241 catgcgggac gtctccgcag atcttattac aaaagtactg gcggcaagca cggcaaaaat
      301 cgtcgttccg cgtgaacggc gggaaccgcg tgcgcagccc gttgcgccta cagcggcgcg
      361 cccgatggag tcgtttgcct cgcgctggag gaaaggacgc cccgagatgg tcgccatagc
      421 ctcatcaacc ggcggtccca gagcgttgca gcaggtgctt tctcagatac ccggggattt
      481 cccggttccc attgtcattg tgcagcacat gccgaaggat tttaccctct ctttcgcaaa
      541 acgactggat tctctgtctg aactcgatgt cgtcgagggg gccgaaggaa cggaattacg
      601 tccggggcta gccgtagtgg ctcctggagg gtatcatatg gtagtgcgga agaaatcgcc
      661 gggaaaattc gcttgcgctc tttccgatgc gccccctctt ctttccgtca agccgtcggc
      721 gaacatcatg tttttgagcg ttgccgacga gatcggaggg aatacggtcg gggtgattct
      781 caccggtatg ggacgggacg gggccgacgg cgcagccgcg ctcaagatga aaggggcgcg
      841 catcatcgca gagtcccagg agacgtgcgt tgtgtacgga atgccgaagg ctgcggtgga
      901 acagggcgtt gtggacgagc ttcttccttt gtactcaata cccgaggcaa tgatccgaag
      961 cgtgagagaa taacgacatg aaaagacttt acaaggctgg agatgatctg ccatgacgaa
     1021 tatggatatg agccagtata tgggagcttt cctcgacgaa gcgggggaca atctgaagca
     1081 cctcgacgat cttacgctcg tgatcgaaaa agacccgggg aattcggagg ctatcgccga
     1141 gatcttccgg tcggcgcaca ccctcaaagg gatgtccgcg acgatggggt tcgacaggat
     1201 ggcttccctc acccacgcca tggaggacat gctcgacgcc gtccgaaaag gagagtactc
     1261 gttgagcgcg ggggatatcg acctgctctt tcgttccctt gataccctgc aatcgatggt
     1321 tgacgcaata cggtcggttg gaagcgacgc atctgtggat accgacgatc tcgtgaaatc
     1381 tatacgagcc gcgggggcca acagaggagc tgaaccgcct gcggcggaag aaaaaactct
     1441 gaacgacgag ctctccggcc aggagcagaa gtgggttgc
//
LOCUS       DAOSWW010000781         1477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77523_length_1477_cov_2.844585, whole genome shotgun sequence.
ACCESSION   DAOSWW010000781 DAOSWW010000000
VERSION     DAOSWW010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77523_length_1477_cov_2.844585"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1477
                     /locus_tag="PK442_12985"
     CDS             <1..>1477
                     /locus_tag="PK442_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO88612.1"
                     /translation="VLIGDPGVGKTAIVEGLAGRIVKGDVPEGLKDRSIFALDMGSLV
                     AGAKFRGEFEERLKAVLNEVKESDGRIILFIDELHTIVGAGAAEGAIDAGNMLKPLLA
                     RGELHCIGATTVDEYRKRIEKDAALARRFQPVLVEQPTVEDTVSILRGLKDRFQVHHG
                     VKIRDNALVAAAVLSNRYITERFLPDKAIDLVDEACALIRTEIDSLPAELDAVSRKVM
                     QLEIEEAALNKEKDAASVERLKALQKELQEAREEQTGLRAQYEAEKQGIGVVRELRER
                     IESIRRDIEKAEREYDLNRAAELKHGTLPQLEAELREREKALENGHAGPKILREEVTE
                     EEISEIVSRWTGIPVTRLLEGEKEKLLQLDEVLHRRVVGQDEAVELVADAVLRARSGI
                     KDPRRPIGSFIFLGPTGVGKTELAKTLAESLFDSEENLIRLDMSEYMEKHSVSRMVGA
                     PPGYVGYDEGGQLTEAVRRRPYSVILFDEIEKAHPDVFNILL"
BASE COUNT          299 a          431 c          518 g          229 t
ORIGIN      
        1 gtgctcatcg gcgaccccgg cgtcggcaag acggccatag tcgaggggct cgccgggcgt
       61 atcgtgaagg gcgacgtccc cgaggggctg aaggaccgtt ccatcttcgc gctcgatatg
      121 gggtcgctcg tcgcgggggc caagttcagg ggcgagttcg aggagcggtt gaaagcggtg
      181 ctcaacgaag ttaaggagag cgacggccgg atcatcctct tcatcgacga actgcatacc
      241 atcgttggcg cgggcgccgc ggaaggcgcc atcgacgcgg ggaacatgct caaacctctg
      301 cttgcacgcg gcgaactgca ctgtatcggc gcaaccacag tggacgagta ccgcaagcgc
      361 attgaaaagg acgcggcgct cgccaggcgc ttccagcctg ttctggtgga gcagccgacc
      421 gtcgaggata cagtgtccat actgcggggc ctgaaggacc gctttcaggt gcatcacggt
      481 gtgaagatac gcgacaacgc tctcgtcgcg gcggcggtgc tctccaaccg ctatatcacc
      541 gagcgatttc tcccggacaa ggccatcgac ctggtggacg aggcgtgcgc gctcatccgt
      601 acggagatcg actcgcttcc cgcggagctt gacgccgtct cgagaaaggt gatgcagctt
      661 gaaatagagg aggccgcgct caacaaggag aaggacgcgg cgagcgtcga acgtttgaag
      721 gcgctccaga aggagctgca ggaggcgcgc gaggagcaga ccggcctgcg cgcgcagtac
      781 gaggccgaga agcagggaat tggcgttgtc cgcgagctgc gggagcgcat cgaatcaatc
      841 aggcgcgata tcgagaaggc agagcgcgag tacgacctga acagggccgc cgagctgaag
      901 cacggcacgc ttccacagct tgaggccgaa ctgcgcgagc gggagaaggc gctggagaac
      961 gggcacgccg ggccaaagat cctccgcgag gaggtcaccg aggaggagat ttccgagatc
     1021 gtcagccgct ggacgggcat ccccgtcaca cgccttcttg aaggggagaa ggagaagctg
     1081 cttcaactcg acgaggtgtt gcatcggcgc gtcgtcggtc aggacgaggc cgtcgagctg
     1141 gtggccgacg cggtgctccg cgcccgctcc ggcatcaagg acccgcggcg gccgatagga
     1201 tcgttcatct tcctggggcc cacgggcgtc ggcaagacgg agctggccaa gacgctcgcc
     1261 gagtcgctct tcgacagcga ggagaatctg atccggctgg atatgtcgga gtacatggaa
     1321 aagcactccg tctcccgaat ggtgggagcg cctccggggt acgtcgggta cgacgagggg
     1381 gggcagctga ccgaggcggt gcgtcggcgg ccgtactccg tcatcctttt cgacgagata
     1441 gaaaaggcgc atcccgacgt cttcaacatc ctcctgc
//
LOCUS       DAOSWW010000782         1476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77604_length_1476_cov_3.551724, whole genome shotgun sequence.
ACCESSION   DAOSWW010000782 DAOSWW010000000
VERSION     DAOSWW010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77604_length_1476_cov_3.551724"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PK442_12990"
     CDS             complement(<1..402)
                     /locus_tag="PK442_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOO88613.1"
                     /translation="MRYGLVGASGRMGGEIRKAFAAHELTFSLDVGGETHDAAPQVLV
                     DFSLPAAFPSTVAVARRFACPLVIGTTGLSAEQLAELRTLGEQVAVVQSFNFATGVTL
                     LKMMLREFAPLFADWDVEISETHHNKKKDAPS"
     gene            complement(399..1283)
                     /gene="dapA"
                     /locus_tag="PK442_12995"
     CDS             complement(399..1283)
                     /gene="dapA"
                     /locus_tag="PK442_12995"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOO88614.1"
                     /translation="MFGGTGTAVVTPFKDGSVDYESFERFLNWQIEGGVEFLVVLGTT
                     GESPTITADERAEIIKFAVKAAAGRVRVVIGTGTNSTASTILLSQQAEGLGADGLLVV
                     GPYYNKPTQEGFYQHFKAVAESVKTPIIVYNVPGRTGSNIAPETVYRLSKLRTIAGVK
                     EAAGDMGQIDMLVRLIKKERPDFAILSGNDDQAFHLVNSGGDGVISVLSNVMPRETSD
                     MIRLALAGEVEKARALHHRMFPLMKNLFVEANPIPVKYAASRLGFCRNELRLPLVPAS
                     EKCVAIVDASMKECGTAL"
BASE COUNT          266 a          496 c          420 g          294 t
ORIGIN      
        1 cgagggggcg tcctttttct tgttatggtg tgtctcgctg atctcgacgt cccagtccgc
       61 gaagagcggg gcgaactcgc ggagcatcat cttcagcagc gtgacgccgg tcgcgaagtt
      121 gaagctctgc accaccgcga cctgctcccc gagcgttctc agctcggcga gctgctccgc
      181 ggagagaccg gtcgtcccga tcaccagcgg gcaggcgaag cgccgcgcaa ccgcgaccgt
      241 tgaggggaag gccgccggaa gggagaagtc gaccagcacc tgcggcgcgg cgtcgtgcgt
      301 ttcaccgccg acgtcgaggg aaaaagtaag ttcgtgcgcg gcgaaggcct tcctgatctc
      361 gccgcccatc ctccccgaag cgcctacaag accgtatctc atagcgccgt tccgcactcc
      421 ttcatggagg cgtcgacgat cgcaacgcac ttctccgacg ccggaacgag cggcaggcga
      481 agttcgttgc ggcagaagcc gagcctgctg gccgcgtact tcacggggat cggattcgcc
      541 tcgacgaaga gattcttcat gagcgggaac atcctgtgat gcagcgcacg cgccttctcc
      601 acctctcctg caagagccag gcggatcatg tcggacgtct cgcgcggcat cacgttggag
      661 agcactgaga ttacgccgtc gccgccgctg ttcacaaggt ggaacgcctg atcgtcgttc
      721 cccgaaagga tggcgaaatc ggggcgttcc ttcttgatga ggcgcacgag catgtcgatc
      781 tgccccatgt ctcccgccgc ttctttgacg ccggcgatcg tgcgaagctt gctcaggcgg
      841 tacaccgtct ccggcgcgat gttcgacccg gtccgccctg gcacgttgta gacgatgatc
      901 ggggtcttca cgctctcagc gaccgccttg aagtgctggt agaaaccctc ctgcgtcggt
      961 ttgttgtagt acggccccac gaccagcaaa ccgtcggcgc ccaacccctc cgcctgctgc
     1021 gacagcagaa tcgtgctcgc cgtcgaattc gtccccgtgc cgataaccac gcgcactctg
     1081 cccgccgccg ccttcacggc gaacttgata atctccgccc gttcgtcggc ggttatagtc
     1141 ggactttcgc ctgtggtccc aaggacgacg aggaactcga cgcctccttc gatctgccag
     1201 ttcaaaaacc gctcgaaact ctcgtaatcc acgctcccgt ccttgaacgg ggttaccacc
     1261 gcagtacctg ttcctccaaa catcgctcgc acctcctgat catagtgtcg gttccctata
     1321 caaaaaggcc cgtccccgaa agggacgagc ctgaactcgc ggtaccaccc tgattggaca
     1381 tgttgtaata tgtcccgctc ggaccttgtt acggaggtcc ataccgtgaa cgtactccga
     1441 cgaacgcccc ttttttcggg cgtgtctttc cgctcc
//
LOCUS       DAOSWW010000783         1476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77605_length_1476_cov_3.432090, whole genome shotgun sequence.
ACCESSION   DAOSWW010000783 DAOSWW010000000
VERSION     DAOSWW010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77605_length_1476_cov_3.432090"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PK442_13000"
     CDS             complement(<1..968)
                     /locus_tag="PK442_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88615.1"
                     /translation="MNDRPQRAPIRGITRKRILPAGWRRRSFFRTIGFCVLLFWLPLL
                     APGIAECADRDAKAHILILHSYHDALPWSKGLNRGIGNAFAHAPFPIELYVEYLDAIH
                     HPGEDILDETTALFKKRYEDIPLDAVLLTDDPAWTFLVERRDLLFPGVPLFFCALNDF
                     HPERLEGISAVTGVAENPDMSGTVELVRKLHPGIREIPVVADRNPGSLLVLKNLERIS
                     RELHGEVTFRALTDAGFPELLEELKRLPEGTPVIFHAFLRDASGATFPSNLTVLGELS
                     ETISSPFYTFKRIDIGHGAVGGAVVSEELLGSLTARMLVDHLNGVP"
     gene            1201..1374
                     /locus_tag="PK442_13005"
     CDS             1201..1374
                     /locus_tag="PK442_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88616.1"
                     /translation="MTDAPMACATISALLLENCGERNIALTPRSLASEMMFARSAALG
                     SLPSVSMATSTRP"
BASE COUNT          292 a          428 c          454 g          302 t
ORIGIN      
        1 aaggggacgc cgttcaggtg gtcgaccagc atccgggctg tcagggagcc cagaagttcc
       61 tcgctcacga cggctcctcc tactgctccg tgcccgatgt cgatccgctt gaacgtatag
      121 aacggagagg agatggtttc ggagagttcc cccagtactg tcagattgct gggaaacgtg
      181 gcgcccgaag cgtcccgcag gaatgcgtgg aatatcacgg gcgttccctc cggaaggcgt
      241 ttcagctcct ccagaagctc gggaaaaccg gcgtcggtca gcgcacggaa cgtcacttcg
      301 ccgtgcagct cgcgagagat acgctccaga ttcttcagga cgagaagact tccggggttg
      361 cggtcggcga cgacgggaat ctccctgatt ccggggtgca gcttccggac cagctccaca
      421 gtcccgctca tgtccgggtt ttcggcgacg ccggtcacgg cgcttattcc ttcgaggcgt
      481 tccgggtgaa agtcgttgag ggcgcagaaa aagagaggta ctccgggaaa gagaagatcg
      541 cggcgctcca ccaggaaggt ccacgctgga tcgtcggtga gcaggacggc gtcaagagga
      601 atatcctcgt acctcttttt gaacagcgcc gtcgtttcgt cgaggatatc ctctcccggg
      661 tggtgaatgg cgtccagata ttccacgtat aactctatcg gaaacggtgc gtgcgcgaag
      721 gcgtttccaa tgccgcggtt cagtcctttg ctccagggca gcgcatcatg gtacgaatgg
      781 aggatcagga tgtgcgcttt ggcgtcccgg tccgcgcact cggcgattcc cggagcaaga
      841 agcggcagcc agaaaaggag aacgcagaaa cctatcgtcc ggaaaaaaga acggcgcctc
      901 cagcccgccg gaagtattct tttacgggtg atcccgcgga tgggggctct ttgcggcctg
      961 tcgttcatgg agcgctcctt tcaactgcgc aagacgaaga ataatgaaat catacgggta
     1021 aaaatccatt cagtcaattt gtaaaagtcc cgaatacgca cagccggaag ggaagccgag
     1081 tccccctccg gctgtgcgta ttcgaaacga gcgtagtgtt cttatttcgc cgcctccgct
     1141 atcgccttcg ccactgctcg gaggttttcc tcccactccg cggcgagcgg gtcggcggtt
     1201 gtgacggatg cgccgatggc ctgcgcgacg atttccgcgc ttcttctcga gaactgcggc
     1261 gagaggaata tcgccttgac gccccgttcc ctggcctccg agatgatgtt cgccagatcg
     1321 gctgccttgg gctccttgcc ctccgtttcg atggcgacct cgacaaggcc gtagcttctg
     1381 gcgagttaag cccatgcggg gtggaagacg aggaaggttg ctccctgcct gccgcggaac
     1441 atcccgacga attcggcgtc cagcgtttta aggcgc
//
LOCUS       DAOSWW010000784         1475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77717_length_1475_cov_2.461972, whole genome shotgun sequence.
ACCESSION   DAOSWW010000784 DAOSWW010000000
VERSION     DAOSWW010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77717_length_1475_cov_2.461972"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..>570
                     /locus_tag="PK442_13010"
     CDS             111..>570
                     /locus_tag="PK442_13010"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO88617.1"
                     /translation="MKHPTPKNGAQKHTDSGRFQAKKGWNSPFTMVPNEILDGTADCP
                     PLSQSQLLIYIALCRHADNATGECWPYYSTLAEKARCTVRTAISSVKYLCEVGLVSKE
                     SRWTECGGQTSNIYTVYLPSQRFAHPEPNEDDFGEDTENSSDKMEETSDTP"
     assembly_gap    571..580
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <581..945
                     /locus_tag="PK442_13015"
     CDS             <581..945
                     /locus_tag="PK442_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88618.1"
                     /translation="HEMISPSPEIKTEPHEIDSRGDETPAVPHETVSPLYKVEQTQEL
                     THEQTQEKGLANLPPEEREARIKSLPPEIREPMLRFYKRLDNSGAIRRGPLDPPVQHT
                     LQGIYGNDGIAATMEAAP"
     gene            955..>1475
                     /locus_tag="PK442_13020"
     CDS             955..>1475
                     /locus_tag="PK442_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOO88619.1"
                     /translation="MKFVEPLRTLHQIEAVLSRFGGPLAHRNKALFLFGVTSALRISD
                     ILAMKWADVLDFEDYDEGNGEVIAFRSTVEVREKKTGKRKVFPLSSAVASVLGEWRRL
                     NRGAWIFSAPGGSRPISRVHAWRVLHRAAEKAGISGIGTHSLRKTWGYHARRSGVSIA
                     LIMSALNHSSEAVT"
BASE COUNT          346 a          445 c          403 g          271 t
ORIGIN      
        1 ccgggaggcg atcatatgag aatgcatcaa cggagccgaa aggctcttcg ttgcaccgta
       61 cccgcatttt tgcagcactc cgcggacctt tcccgcacgg aggcgctgtc atgaaacatc
      121 caaccccgaa gaacggcgcg cagaagcata cggacagcgg acggttccag gccaaaaagg
      181 gctggaactc ccccttcacg atggttccca acgaaatcct cgacggaacc gcggactgcc
      241 ctcccctctc ccaatcgcaa ctgttgatct acatcgctct ctgccgccac gcggacaacg
      301 ccaccggcga atgctggccg tactactcga cacttgccga aaaagccaga tgcaccgtga
      361 ggacggccat ctcctccgtc aagtatcttt gcgaagtcgg cctcgtttcc aaagagtccc
      421 gctggacgga gtgcggcgga caaaccagca acatctacac tgtctatctt ccgtcacagc
      481 gatttgcgca ccccgaaccg aatgaagacg acttcggaga agataccgaa aacagctccg
      541 acaaaatgga agaaacttcg gatacccccc nnnnnnnnnn cccatgaaat gatttcaccc
      601 tcccctgaaa taaagacgga accccatgaa atagattcac ggggggatga aaccccagcg
      661 gtaccccatg aaactgtttc acccttatat aaggtagaac agactcaaga acttactcat
      721 gaacaaaccc aagaaaaagg gctggcaaat ttgccgcctg aagaacgaga ggccagaatt
      781 aaaagcctcc cgcccgaaat acgcgagccg atgctccgtt tttataagcg actcgataat
      841 tccggagcta tacgccgagg tcctcttgat ccgccagtac agcatactct acagggtata
      901 tacggaaacg acggcatagc cgcgacgatg gaggcagccc catgaggccg gattatgaaa
      961 ttcgtggagc ccctgcgtac gctgcaccag attgaggctg ttctctcccg gttcggcggg
     1021 cctctggcgc acaggaacaa ggcgctcttt ctcttcggcg ttacttcggc gctgcggatc
     1081 tccgatattc ttgcgatgaa gtgggccgac gtgctggact tcgaggacta cgacgaaggg
     1141 aacggcgaag tgatcgcgtt ccggtcgacc gtcgaggtac gggagaagaa gactggaaaa
     1201 cggaaggtgt ttccgctttc ctccgccgtg gcgtccgtcc tcggggagtg gcggcgtctg
     1261 aaccgcggcg cgtggatctt ttccgcgccg ggcggatcgc gcccgatctc gcgcgtgcat
     1321 gcgtggcgcg tgctgcatcg agccgccgag aaggctggaa tctccggcat cgggacgcac
     1381 agcctgcgca agacctgggg gtatcacgcc agacgttccg gcgtgtccat cgcgctgatc
     1441 atgtccgccc tgaatcattc gtcggaggcg gtgac
//
LOCUS       DAOSWW010000785         1474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77822_length_1474_cov_2.080338, whole genome shotgun sequence.
ACCESSION   DAOSWW010000785 DAOSWW010000000
VERSION     DAOSWW010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77822_length_1474_cov_2.080338"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PK442_13025"
     CDS             complement(<1..1437)
                     /locus_tag="PK442_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO88620.1"
                     /translation="MQFLASTATMADGEHFMKLLTGVDFYFIGPERDGTPTFEKRIVH
                     CSISSAGDGYRSFLRKIGRDVLNCDGNERFLIFTDSRKRVEELAADLQRSLRGEKEEK
                     TDDRSAEPLEEVESLIANAQILPYRAGYEDNCREKIQEALTQAKLKGVVSTSALELGI
                     DIGNIGLVILIGAPADARSFWQRAGRTGRDCPGLVIILDTDDSVYRIGLRRYLARDFR
                     PNTLYLDNEYLRYANVLCAAEEQRRASSARCSEAPFSDLNPVFLHLLHNELHPVYPVP
                     QDLYPLKQQAANGPHHAFPLRTCAEKNYVLTLGYGWSGSLSYSQLLREGYPCAVYRHL
                     AQAFRVNRIYHGPGEIVVRKSKTIARTSPKIQKVVFPLFQAIHQLLRSKSFYVCESEL
                     QVAERVIGFRETIGRNLVRDETYGMESSFSQYPLNRFIRTSGVSFLLSGTPGKSGAGK
                     YVCDAFCSVCQIREGDVDWGVFVGKGENF"
BASE COUNT          346 a          424 c          373 g          331 t
ORIGIN      
        1 gaaattctct ccctttccga cgaaaacacc ccagtccaca tctccttccc ggatctggca
       61 gacggagcag aatgcgtcgc agacatactt gcccgcgccg cttttcccag gtgttcccga
      121 aagaagaaag ctgacgccgc tcgtcctgat aaagcggttc agaggatact gtgaaaacga
      181 actctccatt ccgtaggtct cgtcccggac cagattccgt ccaatagttt cgcggaaccc
      241 aatcacacgt tccgccactt gcaattcgct ttcacaaacg taaaagctct ttgaccgcag
      301 cagttgatgt attgcctgaa aaagaggaaa cactactttt tgaatctttg gcgatgttcg
      361 tgcgatagtc ttgctcttcc gaacgacaat ctctcccgga ccatggtata tccgattgac
      421 acgaaatgcc tgcgccagat ggcgatagac cgcgcagggg tatccttcgc ggagcaattg
      481 ggaataggag agcgaaccgc tccagccgta cccgagcgtc agcacatagt tcttttccgc
      541 gcacgtacga agcgggaaag catggtgcgg gccgttcgcc gcctgctgtt tcaaagggta
      601 gagatcctga ggaaccggat agaccgggtg cagctcgtta tgcagcagat ggagaaagac
      661 cgggttcaaa tcgctgaacg gcgcttccga acagcgtgca gaagaagctc gccgttgttc
      721 ttcggcagcg cagagcacgt tggcgtagcg aagatactcg ttatccagat agagcgtatt
      781 cggcctgaag tctcgcgcta aataccttcg caagccaatc ctgtagacgg aatcgtcggt
      841 gtcgagaata atcacaagcc cggggcagtc ccgtccggtt cgtcctgccc gttgccagaa
      901 tgaacgcgca tcggcaggcg ctccgattaa aataacaaga ccgatgttgc ctatgtcgat
      961 gccaagctcc agcgcgcttg tcgagacaac ccccttcagc tttgcctgtg tcagcgcctc
     1021 ctgaatcttc tcgcggcagt tgtcctcgta tcctgcccgg taggggagga tttgcgcgtt
     1081 tgcaatcaat gattcaacct cttcaagagg ttctgccgag cgatcatcgg ttttctcctc
     1141 tttttcaccg cggagactgc gttgaagatc cgcagccagt tcttcaaccc gtttccggga
     1201 gtcggtaaaa atcaagaagc gttcgttccc gtcgcaattc aatacatcgc gcccgatctt
     1261 ccgcagaaaa gaacgatagc cgtcaccagc ggaagaaatg gagcagtgga cgatgcgctt
     1321 ttcaaatgtg ggagttccgt cccgctccgg gccaataaaa taaaaatcca cacctgtcaa
     1381 cagtttcata aaatgctccc cgtccgccat tgtcgccgtg ctcgccaaaa actgcattcc
     1441 cgtcgctccg gacggtgtac ccttatcgga tgag
//
LOCUS       DAOSWW010000786         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77883_length_1473_cov_2.669252, whole genome shotgun sequence.
ACCESSION   DAOSWW010000786 DAOSWW010000000
VERSION     DAOSWW010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77883_length_1473_cov_2.669252"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1012)
                     /locus_tag="PK442_13030"
     CDS             complement(47..1012)
                     /locus_tag="PK442_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO88621.1"
                     /translation="MPSELSKTYDPKAVEKKWYGFWKNGGNFHAPDAPSGKRYCIVIP
                     PPNVTGALHMGHALNNTLQDILIRWKRMSGYASLWQPGTDHAGIATQNVVEREILKTE
                     KKRRQEIGREALISRIWEWKELYGDRILTQLEAMGCSCDWERTRFTLDDGLSKAVREC
                     FVRLYDEGLIYRGKYIVNWCPRCHTALADDEVDHKDERGHLWHIRYPLREEGTGNREL
                     GYVVVATTRPETMLGDTAIAVNPKDERYASLVGKMAHLPETNREIPIIADDFVDAAFG
                     TGAVKVTPAHDPNDFQIGERHNLPRINVMNADATMSEEAGEKYGG"
     gene            complement(1149..>1473)
                     /locus_tag="PK442_13035"
     CDS             complement(1149..>1473)
                     /locus_tag="PK442_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO88622.1"
                     /translation="LACAVASTALDVIVDENLPARSAELGDYFRGEIRKADIARVKEI
                     RGKGLLTGVELHEKARPFSEELMHRGILAKDTHGTTIRFAPPLVITKDELAEALKTIV
                     AVLGK"
BASE COUNT          215 a          473 c          467 g          318 t
ORIGIN      
        1 gctcggtcga gttcctccac gatcttcctt ctgcactcgg ccgtcgtcat cccccgtatt
       61 tctcgccggc ctcctcgctc atcgtcgcgt ccgcgttcat cacgttgatg cgcggcaggt
      121 tgtggcgctc gcctatctgg aagtcgttcg gatcgtgcgc gggggtcacc ttcaccgcgc
      181 cggtcccgaa ggcggcgtcg acgaagtcgt ccgcgattat gggtatctcg cggtttgtct
      241 ccggcaggtg cgccatcttc cccacgagcg aggcgtagcg ctcgtccttc gggttcaccg
      301 cgattgcggt gtcgccgagc atcgtctccg ggcgcgtcgt cgccaccacg acgtagccca
      361 gttccctgtt ccccgttccc tcttccctaa gcggatagcg tatatgccaa aggtggccgc
      421 gctcgtcctt gtggtcgacc tcgtcgtcgg cgagcgccgt gtggcagcgc gggcaccagt
      481 tgactatgta cttgccgcgg tagatgagcc cctcgtcgta gaggcgcacg aagcactcgc
      541 gcaccgcctt cgagaggccg tcgtcgagcg tgaagcgcgt tcgctcccag tcgcaggagc
      601 agcccatcgc ctcgagctgc gtaaggatcc ggtcgccgta gagctccttc cactcccaga
      661 tccggctgat gagcgcctcg cggccgatct cctggcggcg cttcttctcc gtcttcagta
      721 tctcgcgctc gacgacgttc tgcgtcgcga tccccgcatg gtcggtgccg ggttgccaga
      781 gcgacgcata cccgctcata cgcttccagc gtatgaggat gtcctggagg gtattgttga
      841 gggcgtgccc catgtgcagc gcgccggtga cgttcggcgg cgggatgact atgcagtaac
      901 gctttcccga tggggcgtcc ggggcgtgga agttgccgcc gtttttccag aaaccgtacc
      961 acttcttctc aactgccttc gggtcgtagg tcttggatag ttctgagggc atcgtcatgg
     1021 cctttccgtg gaaaaacagc gaaaatgctt aagaaattcg agtggatatg tcaacagccg
     1081 caaagggaag gggagcgtcc caccggatcc gatcgggaac gctccccctc cgtttgtgat
     1141 aagagcgtct attttccgag aacggcgact atggtcttca gcgcctcggc gagctcgtcc
     1201 tttgtgatga cgagcggcgg ggcgaagcgg atcgtcgtgc cgtgcgtgtc cttcgcgagg
     1261 atcccgcggt gcatgagctc ctcggagaag gggcgggcct tttcgtgcag ctcgacgccc
     1321 gtgaggagcc ccttgccgcg gatctccttt acgcgcgcga tatccgcctt gcggatctcg
     1381 ccgcggaagt agtcgccgag ctctgccgag cgcgccggca ggttctcgtc gacgatgacg
     1441 tcgagcgccg tggatgcgac cgcgcacgcg agg
//
LOCUS       DAOSWW010000787         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77906_length_1473_cov_2.217207, whole genome shotgun sequence.
ACCESSION   DAOSWW010000787 DAOSWW010000000
VERSION     DAOSWW010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77906_length_1473_cov_2.217207"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PK442_13040"
     CDS             <1..691
                     /locus_tag="PK442_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO88623.1"
                     /translation="GDIASIVVWGQSRKMSKHPEEYGKGSLEGLAVASTANNSVIGGA
                     LTTMLALGIPGDSVSAILIGSLMMYGMQPGPKMFTDNQHFVVTIMLLMLIANVLILLF
                     GLLTAKLSAKVLNVRKETVWVSVVVLCTVGSFALNNSFFDVLIMGGAGILGFLFKRGG
                     FVPGPFILALLLGGLLESNLRRALVMSQGSYSIFFTRPVTVTLLALTALSILFPLIRN
                     HFSAAKIKERD"
     gene            694..>1473
                     /locus_tag="PK442_13045"
     CDS             694..>1473
                     /locus_tag="PK442_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOO88624.1"
                     /translation="MEKKERSEGWKMQSVVKAFGLLKLFTGKNPEWTFNQLLQEKRCP
                     KSTLSNMLRTLEFCGVLEKEKTSQLYRLGLDVLEMSYSVRKALPIIEYAMKFLDDVQQ
                     KTGGIVYFTIPKDGRVLYLDASYPANKNVTYSITGKTLDMHCTGVGKAMLSKMSDEQI
                     RRIIAEHGMKQYTPRTITDFDELMEEIGRIRARGYALDFGEESQGVKCVAVPVVSGRM
                     LLGAISISGSIISLKEEQYEEYAEMLFEIANILADKADMFPR"
BASE COUNT          306 a          389 c          487 g          291 t
ORIGIN      
        1 cggagatatc gcgtcgatcg tggtctgggg gcagtcgcgc aagatgtcga agcatcccga
       61 ggagtacgga aagggttcgc tggaagggct ggcggtcgcg tctaccgcga acaacagcgt
      121 gatcgggggc gcgctgacga cgatgctcgc gctcggcatt ccgggcgact cggtcagcgc
      181 gatcctgata gggtcgctga tgatgtacgg aatgcagccg gggccgaaga tgttcaccga
      241 caaccagcac ttcgtggtga ccatcatgct gctgatgctg attgcgaacg tgctcatcct
      301 gctcttcgga ctgctcaccg cgaagctctc cgccaaggtg ctgaacgtgc ggaaggagac
      361 ggtgtgggtg tcggtcgtcg tgctgtgcac ggtgggctcc ttcgccctca acaacagctt
      421 cttcgacgtc ctgatcatgg ggggcgccgg aattttaggc tttttgttca agagaggcgg
      481 tttcgttccg gggccgttca tcctggcgct tctgctggga ggactgctgg aatcgaacct
      541 tcggcgggcg cttgtgatgt cgcagggcag ctacagcatc ttcttcacgc gccccgtcac
      601 ggtgacgctg ctcgctctga cggcgctctc catactgttc cccctgatca gaaatcattt
      661 ttccgcggcg aagataaagg aacgtgattg acggtggaaa aaaaggagcg atccgagggc
      721 tggaagatgc agtccgtagt caaggcgttc gggctgctga agctgtttac ggggaaaaat
      781 ccggagtgga cgttcaacca gctgcttcag gagaaacggt gtccgaagag cacgctctcg
      841 aatatgctgc ggactttgga gttctgcggc gttctcgaga aggagaagac gagccagctc
      901 taccgactgg ggcttgacgt gctcgaaatg agctacagcg tccgcaaggc cctcccgatc
      961 atcgagtacg cgatgaagtt tttggacgac gtgcagcaga agaccggcgg gatcgtctac
     1021 ttcaccattc ccaaggacgg cagggtgctc taccttgacg cgagctatcc ggcgaacaag
     1081 aacgtgacct actcgatcac cgggaagacg ctggacatgc actgcaccgg ggtgggcaag
     1141 gcgatgctga gcaagatgtc ggacgagcag atccggcgga ttatcgccga gcacggtatg
     1201 aaacagtaca ccccgaggac gatcaccgac ttcgacgagc tgatggagga gatcgggaga
     1261 atccgcgcgc ggggctacgc cctggacttc ggggaggagt cgcagggtgt gaagtgcgtc
     1321 gccgtccccg tcgtttcagg acggatgctt ctgggagcga tcagcatctc ggggtcgatc
     1381 atcagtctca aggaagagca gtacgaggag tacgccgaga tgctgttcga aatcgccaac
     1441 atactggcgg acaaggcgga tatgttcccg agg
//
LOCUS       DAOSWW010000788         1472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_77961_length_1472_cov_2.527170, whole genome shotgun sequence.
ACCESSION   DAOSWW010000788 DAOSWW010000000
VERSION     DAOSWW010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77961_length_1472_cov_2.527170"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1472)
                     /locus_tag="PK442_13050"
     CDS             complement(<1..>1472)
                     /locus_tag="PK442_13050"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOO88625.1"
                     /translation="PLVMAGSLALALRSLPIAPYQSFLKQAFGGALERLLGFVQNGTL
                     DILSLLLTFAIAHAYALKKNESLKEKLSPLIVSITALSSFIIFRCAYSGESLITAFGN
                     TTVAGGVVSALLASMLFVRLRRIQLFRIGVYSADAYAFYNSSLSSIFPAMTTIALFAL
                     SALLYKEFLAPANLTGGWAGDMYAALLEKGPVLGSALLFVFFVHLMWCFGIHGNNVLE
                     AVAQDLFVPALQVNQELVASGQLPTEIVTKTFLDTFVLMGGSGATLCLVAGILLFSKS
                     RSERRLIKLSSLPLLLNVNELIIFGIPIVFNPVYFIPFTLLPVLLTATSFFAVSLGLV
                     PHTVNHVEWTTPVFVSGYIATGSFRGVALQLFNIVLGTLCYLPFIRLARSATDKKISN
                     ALDKAYDMLENDPLRFTMHGQTETADFLRFLALDLENDLQTEKLRLHYQPQVDHDGRV
                     FGVEALLRWTHENYGPVAPQLAVRLAESTGLIGKLGYWIL"
BASE COUNT          362 a          388 c          453 g          269 t
ORIGIN      
        1 tgagtatcca gtagcccagc tttccgataa gccctgtgga ttcagccagc cgaacggcca
       61 gttgcggcgc aacgggaccg tagttttcgt gcgtccaccg cagcagggct tccactccga
      121 agacgcgccc gtcgtgatcc acctggggct gatagtgcag gcgcagcttt tcggtctgga
      181 ggtcgttctc aaggtccagc gccaggaaac ggagaaaatc cgccgtttcc gtctgcccgt
      241 gcatcgtaaa gcgcaacgga tcgttctcaa gcatatcgta ggctttgtcc agcgcattcg
      301 agattttttt atcggtcgct gatcgcgcca gacgtatgaa cggcagatag cagagcgttc
      361 ccaggacgat gttgaagagt tgcagcgcaa cgccgcgaaa cgaccccgtt gcgatgtacc
      421 cgctgacgaa gacgggcgtc gtccactcca catgattgac ggtgtgcgga accagcccga
      481 gcgagaccgc gaagaaggac gtcgccgtca gcagaaccgg gaggagcgtg aaagggatga
      541 agtagacggg attgaagacg atcggaattc cgaaaatgat cagctcgttg acgttgagca
      601 gaaggggcag cgaggagagc ttgatcaggc ggcgctccga gcggcttttg ctgaagagca
      661 gaattcccgc cacaaggcag agcgtcgcgc cgcttcctcc catcaggacg aatgtgtcca
      721 gaaatgtctt ggtcacaatc tccgtgggaa gctgccctga ggcgacgagc tcctgattta
      781 cctgaagcgc cggaacgaaa agatcctgcg cgaccgcttc gagcacgttg ttgccgtgga
      841 tgccgaaaca ccacatcaag tggacaaaaa agacaaagag gagcgcgctt cccagaaccg
      901 gacccttttc gagcagcgca gcgtacatgt ctccggccca tcccccggtc aggtttgccg
      961 gcgccagaaa ctctttgtat agaagggcgg aaagggcaaa aagggcgatc gtcgtcatgg
     1021 cagggaagat ggacgacagc gacgagttgt aaaacgcgta ggcatccgca gagtaaactc
     1081 cgattctgaa gagttgtatt ctcctcaacc ggacgaagag cattgacgcg agcagggctg
     1141 aaacgactcc ccctgcgacc gttgtgttgc cgaaggcagt gatcaacgat tcgcccgagt
     1201 acgcgcagcg aaaaatgata aacgagctca atgcggttat ggaaactatg agcgggctga
     1261 gtttctcttt gagagactcg ttctttttca aggcgtaggc atgagcaata gcgaaggtca
     1321 gcagcagaga gaggatgtcc agagttccgt tctgcacgaa gccgagcagg cgttccaagg
     1381 cgccgccgaa agcctgtttc aaaaagcttt gatacggagc gatggggaga ctgcggagag
     1441 cgagggcgag agaaccggcc atcaccaggg ga
//
LOCUS       DAOSWW010000789         1471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_78019_length_1471_cov_3.363701, whole genome shotgun sequence.
ACCESSION   DAOSWW010000789 DAOSWW010000000
VERSION     DAOSWW010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78019_length_1471_cov_3.363701"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..142)
                     /locus_tag="PK442_13055"
                     /pseudo
     CDS             complement(1..142)
                     /locus_tag="PK442_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
     gene            complement(320..949)
                     /locus_tag="PK442_13060"
                     /pseudo
     CDS             complement(320..949)
                     /locus_tag="PK442_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
     gene            1217..>1471
                     /locus_tag="PK442_13065"
     CDS             1217..>1471
                     /locus_tag="PK442_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOO88626.1"
                     /translation="MKGFGAGVRAISYYLPPKVVDNKALVEEFGTWTEEKIYQKTGIR
                     ERHIVDGELVSDLATRAGEKFFEEHGIGRDEIDFLLLCTES"
BASE COUNT          282 a          450 c          464 g          275 t
ORIGIN      
        1 cgaggaactg gatcgcgtcc cccatcgagc cgccggtcgc gagcatcggg tcgagaacgt
       61 agacctgccg accggtgagg tcctcgggaa gccggttggc ataggtcgtg atggcgaggg
      121 tctcctcgtc gcgctgcatc ccttcgatgg agagggattc cctccgccag cggacgatgt
      181 cgcacgggat gaactccacg ccgcctctcc cgccgggatg cccgcccgcg gagcccatac
      241 ttaccgagaa gacgcctttc ttgcccgtct cgccgatatg ccgcccctga atgttgccgg
      301 ggtagacgac cagcggatcg tggcagagaa tttccgcgga gtgcacgtgc cccagcgccc
      361 agtagtccat ccccgccgcg cggagatcgt ccagcgagca gggggcgtag ttttcgtgcc
      421 ccttccttcc tccgacgttg ctgtgaagca gggcgatgtt cacgccctcc gttcgccgtc
      481 ccgcgaagcc gcgcgccagg ttctccctga cgtcgcgcac ggggtagctg cacccgtgca
      541 ccgttcccac ccgctcgccc ttcaccgtca gcggcacgga ctcgacctcc gacgagccga
      601 accggtacgc caagggaggc aggtcgcggt ccagctccca cccggagagc ggatcgtggt
      661 tgccgtgcac tgtgaacgac gcgatccccg cgtcggaaag ccgtttcagc tgttcgagga
      721 agaagagctg cgcacgtacg cttctgtcct cgccgtcgta cacatctccg gagatcagga
      781 caaagtccgc ctcgttccgc agcgcgcagt cgacggcgtt cctgaaggcg gagaaggagg
      841 ccgcgcgcaa aaagctcccc agcgcggggg aggtctccgc aagcccggag aaggggctgt
      901 cgagatgaag atccgcacaa tgtatgaatt tcagcgtgcc gctgcccatg agagcgccac
      961 tcctttcatt cggagcaccg gggaacgcgt cggagcgtct tcccggagcc ggtcttcttc
     1021 gcggagcggt tttccgggcc gcgaaaggtg tccttacttt attccacccg ccgtttcttt
     1081 gcaagagcat tcagcgcgca ttttcaggac aacggaagac ttgtcactta tcggtgacaa
     1141 tgggataata gcaccgcgtc gagtatgatg cacattctgt aaataaggaa catctagtta
     1201 tggaggcgtt cgagagatga aaggatttgg agccggagta cgggctattt catactacct
     1261 tccgccgaag gtggtggaca acaaggcgct ggtggaggag ttcggcacat ggaccgagga
     1321 gaagatctac cagaagacgg gcattcggga gcgccatatt gtggacgggg agctcgtctc
     1381 cgatcttgcg acgcgcgccg gtgagaagtt tttcgaggag cacggcatcg gaagagacga
     1441 aatagacttt ctgcttctgt gcaccgagag c
//
LOCUS       DAOSWW010000790         1468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_78273_length_1468_cov_3.624204, whole genome shotgun sequence.
ACCESSION   DAOSWW010000790 DAOSWW010000000
VERSION     DAOSWW010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78273_length_1468_cov_3.624204"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PK442_13070"
     CDS             <1..998
                     /locus_tag="PK442_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOO88627.1"
                     /translation="NVVVVETEEGVCGYGEAPPTAVITGDTNGAIRGAVEERLRPLLS
                     GRETDDLNSLLDIVDTALVHNASAKAAVDIALHDLWGKTLGRPLYELLGGTAREVETD
                     YTISVNAPETMVKDSLEAVADGYSALKIKVGTDFHLDMQRLEEIRAAVGKHVLLRVDA
                     NQGWTPKEAIRTIRFLEDKGLDIELVEQPVKAHDFEGLKMVTDSVDTLILADESVFSV
                     RDAFTVLSMRAADLINIKLMKTGGIRNAVAICGMARACGVECMIGAMMETKISVSAAC
                     HLASALPVITRADLDPPILCASDPVVGGATYRGSSVVPSGGAGLGIEKINGLVLE"
     gene            complement(1054..>1468)
                     /locus_tag="PK442_13075"
     CDS             complement(1054..>1468)
                     /locus_tag="PK442_13075"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOO88628.1"
                     /translation="AARATLAGASGGCQAECGAAAAMGAAALVQMRGGSPEAVGHAVA
                     LAYKSILGLVCDPVGGLVESPCIKRNGMLVSLSALAADMALAGLRSLIPPDEVIDAMG
                     QVGRALPESLRETAGGGLAVTPTARELVAKLLQQE"
BASE COUNT          278 a          430 c          500 g          260 t
ORIGIN      
        1 ccaatgttgt tgtggtggag accgaggagg gagtctgcgg atatggagaa gcgcccccca
       61 cggcggtgat caccggcgat acgaacggcg ccatccgcgg cgcggttgag gagcggcttc
      121 gtccgttgct ctccgggcgg gagacggacg atctgaacag tctgcttgac attgtcgata
      181 cagctcttgt ccacaacgcc tccgcgaaag cggccgtgga tatcgcgctc cacgatctct
      241 gggggaagac tctgggccgc ccgttgtacg aactcctcgg cggcacggcc agggaagtgg
      301 aaacggacta taccatcagt gtgaacgcgc cggaaacgat ggtgaaagac agcctggaag
      361 cggttgcgga cggctactcg gcgctcaaga tcaaggtggg gacggatttt catctcgata
      421 tgcagcgtct tgaggagatc agggcggctg tgggaaaaca tgtgctgctc cgggtggacg
      481 ccaaccaggg gtggactccc aaggaggcga tacgcaccat ccgcttcctt gaggataagg
      541 ggctcgacat cgagcttgtg gaacagccgg tgaaagcgca cgatttcgaa gggctcaaaa
      601 tggtaacgga cagcgtcgat acgctcatcc tcgccgacga gtccgtcttc tccgtgcggg
      661 acgccttcac ggtgctctct atgagggctg cggacctgat caacatcaag ctgatgaaga
      721 ccgggggtat ccgcaatgcc gtcgcaatct gcggcatggc ccgcgcctgc ggcgtggagt
      781 gcatgatcgg cgcgatgatg gagacgaaga tcagcgtatc ggcggcgtgc caccttgctt
      841 cggcgctgcc ggtgatcaca agggcggatc tcgatcctcc gattctttgc gcgtccgacc
      901 ctgttgtggg cggcgcgacg tacagaggtt cgtcggtggt cccctccggc ggagcgggac
      961 tcggcatcga aaagatcaac ggtttagtat tggagtaatc ggaaacgaac agaagcgggg
     1021 cgcgccggaa ttgtccggtt cgccccgctt ctgttattcc tgctgcaaga gcttggcgac
     1081 aagctccctc gcggtcgggg tcaccgcgag gccgcctccc gcggtttcgc gcagcgattc
     1141 cgggagggcg cgtccgacct gccccatcgc gtcgatgact tcgtccgggg ggatgagaga
     1201 gcgtaggccg gcaagcgcca tgtccgcggc cagagcagat agcgagacga gcattccgtt
     1261 tcgcttgatg cagggggatt cgacgagtcc gccgaccggg tcgcagacaa gcccgagaat
     1321 cgacttatag gcgagggcga cggcatgtcc gacggcttcg ggagagccgc cgcgcatctg
     1381 caccaaggcg gctgctccca tcgcggccgc cgcaccgcac tcggcctgac agcctccgga
     1441 ggctcccgcg agcgttgcgc gcgcagca
//
LOCUS       DAOSWW010000791         1460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_79040_length_1460_cov_2.638434, whole genome shotgun sequence.
ACCESSION   DAOSWW010000791 DAOSWW010000000
VERSION     DAOSWW010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79040_length_1460_cov_2.638434"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PK442_13080"
     CDS             complement(<1..129)
                     /locus_tag="PK442_13080"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOO88629.1"
                     /translation="MESVGCVHVYTGTGKGKTTAALGLILRAAGAGLSVLLVQFLKR"
     gene            361..570
                     /locus_tag="PK442_13085"
     CDS             361..570
                     /locus_tag="PK442_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOO88630.1"
                     /translation="MESVYKVIELVGTSSESWEKAAAAAVELASKSLRDLRIAEVTAM
                     DMHLEDGKIMAYRTKLKVSFKFEDK"
     gene            800..>1460
                     /locus_tag="PK442_13090"
     CDS             800..>1460
                     /locus_tag="PK442_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOO88631.1"
                     /translation="MTKRKQVDGNCPGSAGRAAGSCGEETLLEENHRLKLEAAVAREA
                     ASKAMAASIAKSEFLANMSHEIRNPMNGVIGMTGLLLDTRLNGEQRYYAGMVRDSAVS
                     LLSILNDILDFSKIEAGKLELETLDFDLPSLLQDFSATMSLRAREKNVALLCSIAPDV
                     PSLLRGDPGRLRQILNNLVGNALKFTQAGEVELGVFLEEESENFALLRFSVRDTGIGI
                     PE"
BASE COUNT          335 a          374 c          466 g          285 t
ORIGIN      
        1 ccgtttgagg aactggacga ggagcacgga aagccccgcc ccggcggcgc gaagaatcag
       61 gccgagcgcc gccgtggtct ttcccttccc tgttccagtg tagacatgca cgcaaccgac
      121 gctctccata aacgaagccc ctccctgctt tttctctcat tatacggtat ggaaaaggac
      181 gttcgccggg atgcagaaaa cggcgcagcc cttgcagaag caagggtccc gaaaagtatt
      241 tattcagagt gaaatgaaga gtggtaatgg tataattaaa ctattctttt ttccggcggc
      301 gatgctttgc cggcggaaac tggcgggctc ttcggagccg atctggaagg aggagatgac
      361 atggagagcg tgtacaaggt gatcgagctt gtggggacga gcagcgagtc gtgggagaaa
      421 gccgccgcgg cggcagtgga gctggcgtcg aagtcgttga gggacctgcg tatcgcggag
      481 gttaccgcca tggatatgca cctcgaggac ggcaagatta tggcataccg gaccaagctc
      541 aaagtatcgt tcaagttcga agataagtag catgcccctg cgttgccgta ccaggaagcc
      601 gcccgcccga taaagttctt taagcgggcg ggcggcttcg tattcccgat gacctcccat
      661 agagcctgaa aagaacggat gatataatcg aaccgagtgc acgaagcgcg tggtatgaac
      721 ataacggtgt atatcaaatc cggggagagc gggccggatc gctcggagag cggaacattc
      781 acgtcacggg agcgacgata tgacgaagag aaaacaggtt gacgggaact gcccgggaag
      841 tgcgggccgc gccgcgggga gctgcggaga agagacgctc ctggaggaga accaccgctt
      901 gaagctcgaa gctgcggtgg cgcgggaggc tgcttcgaag gcaatggcgg cgagcatcgc
      961 gaagagcgag tttcttgcca atatgagcca tgagatccgg aaccccatga acggcgtgat
     1021 cggcatgacc gggctcttgc tggacacgcg cctgaacggc gaacagcgct actacgccgg
     1081 gatggtccgc gacagcgcgg tctcgttgtt gagcatcctg aacgatattc tggacttttc
     1141 caagatcgaa gcggggaaac tcgagctgga gactctggat ttcgatcttc caagtctgct
     1201 tcaagacttt tccgcgacga tgtcgcttcg cgccagagaa aagaacgtcg cgcttctgtg
     1261 cagcattgct cccgacgttc cctcgttgct tcgcggagat cccgggcggc ttcgccagat
     1321 attgaacaac cttgtgggga acgccttgaa gttcactcag gcgggcgagg ttgagctcgg
     1381 agttttcctt gaagaggaaa gcgagaactt cgcgctcctt cgcttctcgg tgcgtgatac
     1441 gggaatcggc attccggagg
//
LOCUS       DAOSWW010000792         1458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_79197_length_1458_cov_3.243051, whole genome shotgun sequence.
ACCESSION   DAOSWW010000792 DAOSWW010000000
VERSION     DAOSWW010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79197_length_1458_cov_3.243051"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK442_13095"
     CDS             complement(<1..297)
                     /locus_tag="PK442_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOO88632.1"
                     /translation="MTAQKSFFVLGEVRDGRIHSVTRELTGKARELADSVGGRVTTVL
                     LSGGLADSPEDLFRYGADKVLSVEHQSLTLYNQEVETNVLAHVREEQPEVVLAGA"
     gene            complement(310..1116)
                     /locus_tag="PK442_13100"
     CDS             complement(310..1116)
                     /locus_tag="PK442_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOO88633.1"
                     /translation="MKIAVLIKQVPDTDDVRMDPEKGTMIREGVGAIVNPLDLNALQA
                     ALDLKAASAEAAEASSSEVTVITMGPPKAHEALRECIATGADRGVLLSDRAFAGSDTW
                     ATAKVLAAAVEHTGPYDLVLAGEKATDGETGQVGPEVAVLLGIPFSTYVTGLSFSEGK
                     IEVRRMTEEGVQRQRLPFPCLLTVLHALNEPTMPTLAGKKRARRSEIPVLGISDIGLS
                     PEETGLKGSATRVVKIAYPTISRSTEMYDGKRADEGIDRLIEVLKDLAVI"
BASE COUNT          293 a          473 c          420 g          272 t
ORIGIN      
        1 cgcaccggcg agcaccacct cgggctgctc ctcgcgaacg tgcgcgagga cgttcgtctc
       61 aacctcctgg ttgtacagcg tcaacgactg gtgctccacg gagaggacct tgtcggcccc
      121 gtaccggaaa aggtcttcag gcgaatccgc cagtcctccc gagaggagca ccgtcgtcac
      181 ccgcccgccg acgctgtcgg cgagttccct ggccttcccc gtcagttcgc gcgtcacgga
      241 gtgaatgcgc ccgtcccgga cctctccgag gacgaaaaag ctcttctgtg ccgtcatagc
      301 gcgttcctcc taaatcacgg cgaggtcttt gaggacctcg ataaggcggt cgatcccctc
      361 gtccgcacgt ttcccgtcgt acatctccgt ggaacgggag atagtaggat aagcgatctt
      421 caccacgcgg gtcgcggagc ccttgagccc cgtctcctcg ggcgagagcc cgatgtcgga
      481 gattccgagc acaggaatct cgctccgccg cgcccgcttc ttgccggcga gcgtcggcat
      541 ggtcggctcg ttgagtgcgt gaagcaccgt gagcagacag gggaagggca gacgctgccg
      601 ctggacgccc tcctccgtca tgcgtcggac ttcgatcttt ccttcggaga aggagagtcc
      661 ggtgacgtac gtgctgaagg gtatgccaag cagcaccgca acctccgggc caacctgccc
      721 cgtctcgccg tcggtcgcct tctccccggc gagaacgaga tcgtaggggc cggtatgctc
      781 cacagccgca gccaacactt tggccgtagc ccacgtatcg cttccggcga atgcgcggtc
      841 gctgagcagc acgccccggt ccgctccggt cgcgatgcac tcgcggagcg cctcgtgcgc
      901 cttgggcggc cccatcgtaa tcacggtgac ctcgcttgaa gaggcttcgg ccgcctccgc
      961 agaggccgcc ttcaggtcaa gcgcagcctg gagagcgttc aaatccagcg ggttcacgat
     1021 ggccccgacg ccctcgcgga tcatcgttcc cttttcaggg tccatacgaa cgtcgtccgt
     1081 atcgggaacc tgttttatta gcacggcaat cttcacggaa aaacctcctt gcagtggaat
     1141 gatacctgaa acacctttcc tcgctgtacg ataccacaaa gttcaggaga tcggcgatta
     1201 catcgaggga aaaacagctg attttcaggt tccacaaaaa agagggagcg gaaaagcatc
     1261 cgcccccaag catacacaac gcaggatatt cctgtttcaa gctccccttc ccggagagaa
     1321 gaggcgcttt ccgtcgttcc agacatcacg cagcgaatag agcagcataa tgaaggacga
     1381 tacgggcata cagcagtaca gcgcagactt atgaatctgg aagaccgtgg tcaactctgt
     1441 agcgcgaagc gtcagacg
//
LOCUS       DAOSWW010000793         1455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_79479_length_1455_cov_2.925000, whole genome shotgun sequence.
ACCESSION   DAOSWW010000793 DAOSWW010000000
VERSION     DAOSWW010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79479_length_1455_cov_2.925000"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..548
                     /locus_tag="PK442_13105"
     CDS             312..548
                     /locus_tag="PK442_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOO88634.1"
                     /translation="MITVYSTKTCPWCTKVKEYLEGKKIPFEVIDVASDREAAMEMVK
                     KTGQMGVPVTRIGEKYIVGYNPEAIDEEIAQQNQ"
     gene            574..>1455
                     /locus_tag="PK442_13110"
     CDS             574..>1455
                     /locus_tag="PK442_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOO88635.1"
                     /translation="MTRGTRSGSDLLTVSFGMAVDSGADSASTRGVSVEALKECGDSL
                     AKADAWLREGRNEGRDGVGWLSLPYKDIGDIQEAAGWLRGFNAVVQAGIGGSALGNLM
                     LHGALLPPYWNELPEAKRGGPRFYLADNVDPRDNRTVWNLIEPEKTAFIVISKSGSTA
                     ETMANFLFFWDRLKVELGPKRAAEHVLVITDEEKGVLRPFADEIGCRRLVLPSVVGGR
                     FSVLSSVGLLSACALGIDGRKLLAGAAEMDEKLAQRASLFENPAWLLSALSVAHYRRG
                     RNMTVMMPYEDALERFCE"
BASE COUNT          341 a          355 c          432 g          327 t
ORIGIN      
        1 cgatgtaccc catctccttc tggagacccg caagaagcgg tatggtgatc cccttccttc
       61 cccggtatcg gtccgctatt ggaagaaggc gctgctccaa agcggaactc acggaggcct
      121 ccgtcgacat ggtcattttc tctcctcctc gtgctttttc agtgaaatac cccggtgatg
      181 gtatttatta taccgcgtat tgaggacaat ttcccatcca aaaagaaatt cggtatataa
      241 taaatctcga ctgggagtga gatttgatat atactcccct caagtatata tcatttatgg
      301 aggtgtctgt catgataaca gtctattcga cgaagacatg cccgtggtgc accaaggtga
      361 aagagtacct ggaagggaaa aaaataccgt ttgaggtgat cgacgtcgct tcagaccggg
      421 aagcggccat ggagatggtc aaaaaaacag ggcagatggg cgttccggtg acgaggatag
      481 gggaaaagta tattgtaggt tacaatccgg aggccatcga cgaggagata gcgcaacaga
      541 atcagtaagc cgttacagga aggagtggag aaagtgacga gaggaacacg ttcaggctcc
      601 gatctgctta ctgtatcatt tggaatggcg gttgacagcg gggcggattc ggcgtctacc
      661 cgcggtgtga gcgtcgaagc tctgaaagag tgcggcgata gtctggcgaa agccgacgcc
      721 tggcttcggg aaggacgtaa cgagggaagg gacggagtag ggtggctttc ccttccctac
      781 aaggatatcg gcgatataca agaagccgca ggatggctgc ggggattcaa tgcagtggtc
      841 caggcgggaa tcggcggttc cgctctcggt aatctgatgc tgcacggagc gcttcttccc
      901 ccgtattgga acgaactgcc cgaggcaaaa cggggcggtc ccagattcta tcttgcggac
      961 aacgtcgatc cacgggacaa caggacggtc tggaatctta tcgagcctga gaagacagcc
     1021 ttcatcgtca tcagcaaatc cgggagcacc gccgagacga tggcgaattt tcttttcttt
     1081 tgggatcggc tgaaggtgga gctggggccg aaaagagctg ctgagcatgt tcttgtcatc
     1141 accgatgaag aaaagggcgt tctgaggcct tttgccgacg aaatcggctg tcgtcgtctt
     1201 gtacttcctt ccgttgtcgg cggacgcttt tcggtgctct cttcagtagg gcttctttcc
     1261 gcttgcgcac tcgggatcga cggcagaaaa ctcctcgcag gagcggcaga gatggacgag
     1321 aagctcgcgc agcgcgcttc tcttttcgag aacccggcat ggcttctttc cgccctctca
     1381 gtggcgcact accggcgtgg acgcaacatg acggtcatga tgccgtacga agacgcgctc
     1441 gaacgctttt gcgag
//
LOCUS       DAOSWW010000794         1452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_79722_length_1452_cov_3.359341, whole genome shotgun sequence.
ACCESSION   DAOSWW010000794 DAOSWW010000000
VERSION     DAOSWW010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79722_length_1452_cov_3.359341"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..>1452)
                     /locus_tag="PK442_13115"
     CDS             complement(57..>1452)
                     /locus_tag="PK442_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88636.1"
                     /translation="SASNLLGVLNDILDFSKVEAGRFELNILPFSPANLVRESAELFS
                     PQAASKELSLNLRLDGALPPHLLGDPVRLRQILINLLSNAVKFTKEGSVCVSAKLLRD
                     DEKSADILFSVADTGPGIPDALLADIFTPFKQGEAFLNRRYGGTGLGLAISSRFVEMM
                     GGFLLVESTPGTGSSFSFSLTFEKGEERRLEDVKNQERESLPVRTRASDPTVLVVEDN
                     AVNRELVRLLLAKAGYEATLAENGREAVDILSKKRFGLVLMDIQMPVMDGYEATAVIR
                     NPSSDVLDHTVPVVALTAHAVEGFRDECLSRGMDDYLPKPFSSADLLGLLYKWLPMKT
                     RPAAPEPDGTGAQASSPGQDVFDPDGFFSKLFNDREEGKELLELFLESFPEDIELLRQ
                     TINDGEYDKAAKIAHTMKGTAGNACARSMSQCAKALQLAAKEEDRENTRRLFEELLSI
                     FDKVREAMRREMER"
BASE COUNT          265 a          465 c          430 g          292 t
ORIGIN      
        1 gcaggcttgc ctcgttgatg gccgtgtttc aaaaacgttt ccccggaaag tagcggtcag
       61 cgttccatct ccctccgcat cgcctccctg accttgtcga agatggaaag cagttcttcg
      121 aacaacctcc gagtattctc ccggtcctct tccttcgccg ccagctgcag cgccttcgcg
      181 cactggctca tgctccgggc gcaggcgttt cccgccgttc ccttcatcgt gtgggcgatc
      241 ttcgccgctt tgtcgtactc cccgtcgttg atcgtctgcc gcagcagttc gatatcctca
      301 gggaaggatt caaggaaaag ctccaggagc tcctttcctt cttcccggtc gttgaagagt
      361 ttcgagaaga atccgtcggg gtcgaacaca tcctgccccg gggacgacgc ttgagcccct
      421 gtgccgtcgg gctccggcgc cgcgggacgc gtcttcatgg gaagccactt gtagagcaac
      481 cccagcagat ccgccgagga gaacggtttg ggaaggtagt cgtccattcc tctggagaga
      541 cactcgtcgc ggaaaccttc gacggcgtgg gccgtcagcg cgaccacggg gaccgtgtgg
      601 tcgagcacgt ccgacgaggg attcctgatc accgcggtcg cctcgtaccc gtccatgacc
      661 ggcatctgaa tgtccatcag caccaggccg aagcgtttct tcgacaatat atccacagcc
      721 tccctgccgt tttccgccag cgtcgcctcg tacccggcct ttgccagcag cagccggaca
      781 agctcgcggt tcaccgcgtt gtcctccacc accagcaccg tcgggtcgga ggcgcgggtt
      841 cgcaccggaa gggattccct ctcctggttt ttcacatcct ccagccgccg ttcctctccc
      901 ttctcgaagg tgagggagaa agagaatgag gagcccgttc ccggcgtgct ctcgaccaga
      961 agaaaccccc ccatcatctc gacgaaacgg cttgaaatag ccagtccgag cccggtgccg
     1021 ccgtatcgtc tgttcaggaa ggcctccccc tgcttgaacg gggtgaagat atccgccaga
     1081 agcgcgtccg gaattccggg acccgtgtcc gcgacggaga agagaatatc ggcactcttt
     1141 tcatcgtcgc ggaggagctt cgcggagacg cagacgcttc cctctttcgt gaacttcacc
     1201 gcgttcgaga gcagattgat gagaatctgg cgcaggcgga ccggatcgcc gagcagatgt
     1261 ggggggaggg cgccgtccag ccgcagattg agcgagagct ccttggaagc ggcctggggg
     1321 gagaagagct ccgccgactc cctcacgagg ttggccgggg aaaacgggag gatgttcagc
     1381 tcgaaccgcc ccgcttcgac ctttgaaaag tcgaggatgt cgttgagcac gccgagcaga
     1441 ttggaggcgc tt
//
LOCUS       DAOSWW010000795         1451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_79827_length_1451_cov_3.215616, whole genome shotgun sequence.
ACCESSION   DAOSWW010000795 DAOSWW010000000
VERSION     DAOSWW010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79827_length_1451_cov_3.215616"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..>1451
                     /gene="lsrK"
                     /locus_tag="PK442_13120"
     CDS             288..>1451
                     /gene="lsrK"
                     /locus_tag="PK442_13120"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: protein motif:HMM:NF008187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HOO88637.1"
                     /translation="MASGKYLMAIDAGTGSARAVLFDESGRQVAVSQAEWSHKEDPRF
                     PGSMDFDVEGNWKLIVGCVRGALEKSGADPRSIAAIATTSMREAIVLYDGAGKEIWAC
                     ANVDARSSAEVVKLMRLRPGIEEELYAVSGQTFSLNALPRLLWVKENLPDVYESVRAV
                     NMLNDWIAYKLSGVLTAEPSNGCTSGIFDIASRKWKPEIAASCGLRTDIYPAVTESAE
                     IIGRVSADCAALTGLSTETLVVSGGGDAQLGCVGVGAVADGQAAVLGGSFWQYEFNTA
                     VPVMDPKCRVRVNCHAVPGVFQYEAIAFFPGLVMRWFRDAFCQEEKRVEAEGGPDAYM
                     QLAEGASKVPAGSHGMMCAFSDVMNYIAWKHAAPTFSNFSLDAEKFNKYTFFRS"
BASE COUNT          262 a          430 c          475 g          284 t
ORIGIN      
        1 gtcgtgcatg agatcatctc cctgtatcga gtttgttcat gttcctattc tgtattcgta
       61 atgctcgtgt gagcagatga taccatggag cggcggggat tccaagatgt tccgcgggcg
      121 tccggggttg cttcgggaag ccgtcgcttt ccgcaagaaa accgaatttg cattgacaat
      181 actgtaccat ttttcctata atggcatccg tagagacttt ctcacatata tgagatgatt
      241 tcacatatgt gattagagac gccgattctt tccagggggt gagcgctttg gcttcgggta
      301 agtatctgat ggcgatcgac gcgggaacgg gcagcgcgcg ggccgtgctc ttcgacgagt
      361 ccggacggca ggttgcggtg tcgcaggcgg agtggagcca caaggaggat ccccggtttc
      421 ccggctcgat ggatttcgac gtggagggga actggaagct gatcgtcggg tgcgtccgag
      481 gcgcgcttga gaagagcggc gccgatcccc ggtcgatcgc ggcgatagcc accaccagca
      541 tgcgcgaggc gatcgtcctc tacgacgggg cgggcaagga gatatgggcc tgcgccaacg
      601 tggacgcccg gtcctcggcg gaggtcgtga agctgatgcg ccttcgtccc gggatcgaag
      661 aagagctcta cgcggtctcc ggccagacgt tctcgctgaa cgcgctcccc aggctgctct
      721 gggtgaagga gaaccttccg gacgtctacg agtcggtccg cgccgtgaac atgctcaacg
      781 actggatcgc ctacaaactc tccggcgtgc tgacggcgga gccctccaac ggctgcacca
      841 gcggcatctt cgatatcgcc tcaaggaagt ggaagccgga gatcgccgcg tcgtgcgggt
      901 tgcgcaccga catctacccc gcggtgaccg agagcgccga gataatcggc agggtgagcg
      961 cggactgcgc cgcccttacg ggcttgagca cggagacgct tgtcgtctcg ggcggcggcg
     1021 acgcacagct cgggtgcgtg ggcgtgggcg cggtggcgga cggacaggcc gccgtgctcg
     1081 gcgggagctt ctggcagtac gaattcaaca ccgccgtccc tgtaatggac ccgaaatgcc
     1141 gcgtgcgggt gaactgccac gccgtgccgg gcgtcttcca gtacgaggcc atcgccttct
     1201 tccccggcct ggtcatgcgc tggttccgag acgctttctg ccaggaggag aagcgcgtgg
     1261 aggccgaagg aggccccgac gcctacatgc agctcgccga gggcgcctcg aaggttcccg
     1321 ccgggtcgca cgggatgatg tgcgccttct ccgacgtcat gaactatatc gcatggaagc
     1381 acgcggctcc caccttctcc aacttctcgc tcgacgccga aaagttcaac aagtacacct
     1441 tcttccgctc c
//
LOCUS       DAOSWW010000796         1450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_79938_length_1450_cov_2.663799, whole genome shotgun sequence.
ACCESSION   DAOSWW010000796 DAOSWW010000000
VERSION     DAOSWW010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79938_length_1450_cov_2.663799"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PK442_13125"
     CDS             <1..756
                     /locus_tag="PK442_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid metabolism regulator protein"
                     /protein_id="HOO88638.1"
                     /translation="PEVEKKILDAVKAGGKPAVLGFIGGEAKSDSDGVFVCRELEETA
                     AVAAALANGQDGAAARDALYAEYDALKQQAEKIGKRKGYLRGLYSGGTLCYEGQLVAR
                     GILGPIWSNTPLDASKKLADSLKSVEHTMVDYGEDEFTQGRLHPMIDVSLRADRILEE
                     AKDPGVGVILLDVVLGYGCNDDPATGLAEGIRKARKVAGERIAFVASICGVDTDPQNA
                     SRQREILEELGVHVCESNARAARLAAMLVEVAQ"
     gene            753..923
                     /locus_tag="PK442_13130"
     CDS             753..923
                     /locus_tag="PK442_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88639.1"
                     /translation="MKKLLKEGPKVVNIGVEQFYLDVKTQKTEAVVWDWKPPAASGDL
                     LSRLKKLKKGGN"
     gene            926..>1450
                     /locus_tag="PK442_13135"
     CDS             926..>1450
                     /locus_tag="PK442_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOO88640.1"
                     /translation="MIDLEKANQEALSRLLAAQPLIVGMGIAKETVPGMKDKMLLHAG
                     PPLTWETASGPIRGGVMAACMYEGWAKTPEEAEKLAASGAIAFDPCHHHHAVGPMAGI
                     MSPSMPVFVLENRTHGNKAYVTMNEGLGKVLRMGAFGPEVITHLQWMEKTLYPVLKAA
                     IEHAVKKGEEPDVKN"
BASE COUNT          312 a          415 c          509 g          214 t
ORIGIN      
        1 cccgaggtcg agaagaaaat tctcgacgcg gtgaaggcag gaggcaagcc cgcagttctt
       61 ggtttcatcg gcggcgaggc gaaaagcgat tcggacggcg tgttcgtctg ccgcgaactt
      121 gaggagaccg ccgccgtcgc cgccgcgctc gcgaacggac aggacggcgc ggcggcccgc
      181 gacgcgctct acgcggagta cgacgcgctg aaacagcagg cggagaagat cgggaagcgg
      241 aagggctacc tccgcggact ctactccggc ggcactctct gttacgaggg acagctcgtc
      301 gcgcggggga tcctcggccc gatatggagc aacactccgc ttgacgcgtc gaagaagctc
      361 gccgacagcc tcaagagcgt ggagcacacc atggtcgact acggcgagga cgagttcacc
      421 cagggccggc tgcatccgat gatcgacgtg tcgctccgcg ccgaccgcat cctcgaagag
      481 gcgaaggatc ccggagtggg cgtcattctg ctcgacgtgg tgcttggcta cggctgcaac
      541 gacgacccgg cgacggggct tgccgaggga atccgcaagg cgcggaaggt cgcgggagag
      601 agaatcgcct tcgttgcctc gatctgcggc gtggataccg acccccagaa tgcgtcccgg
      661 cagcgggaaa tactggagga gctcggcgtg cacgtttgcg aaagcaacgc cagagcggcc
      721 cgccttgcgg cgatgctcgt ggaggtagcg caatgaaaaa gctgctgaaa gaaggcccga
      781 aagtggtcaa tatcggggtg gagcagttct acctcgacgt aaaaacgcag aaaacggagg
      841 cggtcgtctg ggactggaag cctcccgcag cgagcgggga tctcctctct cgtttaaaga
      901 agctcaagaa ggggggcaac tgaacgtgat cgatctggag aaagcaaatc aggaggcgct
      961 ctcgcgcctg ctcgccgccc agccgctgat agtcggcatg gggatagcga aagagaccgt
     1021 tccggggatg aaggacaaga tgctcctgca tgcggggcct cccctgacgt gggagaccgc
     1081 gtccggtccc atccgcgggg gcgtcatggc ggcctgcatg tacgaagggt gggcgaagac
     1141 gcctgaagag gcggaaaaac tcgccgcgag cggcgccatc gccttcgatc cgtgccatca
     1201 ccaccacgcg gtggggccga tggcggggat catgtcgccg agcatgcccg tattcgtcct
     1261 ggagaacagg acgcacggta acaaggcgta cgtgacgatg aacgagggac tcgggaaggt
     1321 gcttcgtatg ggcgccttcg gcccggaggt cattacacac ctgcaatgga tggagaagac
     1381 gctctaccct gtgctaaagg cggcaatcga gcacgcggtg aagaagggcg aggagccgga
     1441 cgtcaagaac
//
LOCUS       DAOSWW010000797         1449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_80012_length_1449_cov_3.563128, whole genome shotgun sequence.
ACCESSION   DAOSWW010000797 DAOSWW010000000
VERSION     DAOSWW010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80012_length_1449_cov_3.563128"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1009
                     /locus_tag="PK442_13140"
     CDS             236..1009
                     /locus_tag="PK442_13140"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOO88641.1"
                     /translation="MNNTVFSKTEIYLYETLSSTQVTAKQHGRQSGTENVVVISEEQT
                     QGRGRRDRSWISPHGSGLFLSFFFKPRLLPGRLQLVNLAAGLAVKDAVEKTCSVRLAL
                     KWPNDLLWENRKVCGILSEASSESDRIRDCCTGIGINITSPSLENNREAAAVLSQAAF
                     LNDMTGGVHRGVLAATLLDRFADILAVMEIDGGKSLLERYRKECATLGRDVAVITDDG
                     TERGRGVGIGENGELIVQNQHGTVAYCAADIVHATPEES"
     gene            1058..>1449
                     /gene="selD"
                     /locus_tag="PK442_13145"
     CDS             1058..>1449
                     /gene="selD"
                     /locus_tag="PK442_13145"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1091..1093,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOO88642.1"
                     /translation="MRLTEMSTTSGUAAKIGPADLSKILKNIPLPSDERIFSSWASGE
                     DAALWKIDEKRAGILTVDFITPVVDDPLVWGRIAAANSLSDVFAMGGFPLVALNIVGF
                     PVKSLDMSVLEAVLAGGFEKVREAGAFLL"
BASE COUNT          366 a          326 c          394 g          363 t
ORIGIN      
        1 gatgaaagcg cgccccggaa ccgtggacct ctgccgtatt ctcatgaaca caggtggcga
       61 agtcatttca ggaggtactc tcgccgggct gctctcatgt tcccggcaga gcgtctggaa
      121 gagtattcgc agcctcgttg aagaaggctt ttcaatagag tccatccctc aaaaaggata
      181 tatccttcgt gaattgcctc gctatgatct tgctccctcg tatattggcg cgctcatgaa
      241 taatacggtt ttttctaaaa cggaaatcta cctctatgaa acgctttcct caacacaggt
      301 aactgcaaaa caacatggcc ggcagagcgg aacagagaat gtcgtcgtca tttccgaaga
      361 acagacacag gggagaggaa gaagagacag aagctggatt tctccgcacg gaagcggtct
      421 ttttctctcg ttctttttta aacctcgtct tcttcccgga agactacaac ttgtcaatct
      481 cgccgccggg cttgctgtga aagacgcagt ggaaaaaaca tgttccgtaa ggcttgcgct
      541 caaatggcca aacgatctcc tctgggagaa tagaaaggtg tgcggtattc tttcagaagc
      601 atccagtgag tctgatcgta tccgtgactg ttgcacagga ataggaatta atataacgtc
      661 tccgtctctt gaaaacaacc gagaggcggc ggctgtcctt tcccaagcag cttttttaaa
      721 tgatatgacc ggcggggttc accgtggagt cttggcggct acgcttttgg atcgcttcgc
      781 ggatattctt gccgtgatgg agatcgatgg cgggaaaagc cttcttgaga gatacagaaa
      841 agagtgcgca actttaggga gagatgttgc cgtcataacc gacgacggaa cagagagagg
      901 ccggggcgtg ggtatcggcg aaaacggcga gcttattgtc cagaatcaac acggaactgt
      961 agcctactgt gctgcggata tagtgcacgc gactccggaa gaaagttaaa aacatgtgtt
     1021 ataataatgt ttattataac gcaagggaga agatggaatg cgtttgactg agatgtccac
     1081 tacaagcggc tgagcggcta aaataggtcc ggcggacctt tctaagatac ttaagaatat
     1141 ccctctccca tcggacgagc ggattttttc ttcatgggcc tcaggagaag acgcggctct
     1201 ttggaagata gacgagaaaa gggccgggat tcttaccgtt gatttcatca ctccggtagt
     1261 cgacgatcct cttgtttggg gaaggattgc cgctgcaaat tcgctaagcg atgttttcgc
     1321 tatgggcggt ttccccttgg tcgcattgaa catcgtggga tttccggtaa aaagtcttga
     1381 tatgagcgta ttagaggctg ttttggccgg agggttcgaa aaggttcggg aggcgggagc
     1441 cttcctcct
//
LOCUS       DAOSWW010000798         1449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_80017_length_1449_cov_3.259684, whole genome shotgun sequence.
ACCESSION   DAOSWW010000798 DAOSWW010000000
VERSION     DAOSWW010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80017_length_1449_cov_3.259684"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="PK442_13150"
     CDS             <1..1401
                     /locus_tag="PK442_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOO88643.1"
                     /translation="TPEILEELKAAVGAKNVVAGDPDALESYASDEAGIMFTSMPDAV
                     VKAENTEHVSAVMQLASKYKIPVTPRGAGSGLAGAAVPLCGGIVLSMEKMNRILEIDA
                     INRVAVVEPGVVTNELCKAVAEHGFLYAGYPMSTETSFIAGNVATNAGGGKVIRYGNT
                     RRHILGLEVVIPSGTVLNLGGRIRKDTWGYSLMQLIIGSEGTLGVVTKVIVNLEPKPG
                     KTVNLLAAFPDLDSAVDSVAKVIRTGIPVISCELLDKISVNITTKHVNTPLPYQDKAD
                     AFLLIQIEGDSEERLEDGYEKVGNLCLQNGALEVFVAESRTESTAIWNIRQNLAEAMR
                     AYDPYCSLSGDMVVPLSAVPEMVEEIRKAAKERGIAVGILGHIADGNLHPIIFKPESM
                     EPMEWAEYAETFYDDLTSVALRLGGVGSGEHGIGYVKMPIFLHSKSDEELEIMRGIKR
                     SFDPQGILNPGKLLGN"
BASE COUNT          298 a          465 c          461 g          225 t
ORIGIN      
        1 actccggaga tcctcgagga gctgaaggcg gccgtcgggg cgaaaaacgt cgtcgccggc
       61 gaccccgacg ctctggagag ctacgcctcg gacgaggcgg gcatcatgtt cacctccatg
      121 ccggacgccg ttgtgaaggc ggagaacacg gaacacgtct ccgccgtgat gcagctggca
      181 tccaagtaca aaattccggt cacgccgcgg ggcgcgggga gcggactcgc cggggcggcg
      241 gtcccgctgt gcggcggcat cgtcctctcg atggagaaga tgaaccgcat cctcgaaatc
      301 gacgcgatca accgcgtcgc agtggtggag ccgggcgtgg tcaccaacga gctctgcaag
      361 gccgtcgccg agcacggatt tctctacgcc ggctacccga tgagcaccga aaccagcttc
      421 atcgcgggga atgtggccac caacgcgggc ggcggcaagg tcatccgcta cggcaacacg
      481 cgccgccaca ttctcggcct ggaggttgtc attccgtccg gaaccgtcct caacctcggc
      541 ggccgtatcc gcaaggacac gtgggggtac agcctgatgc agctcatcat cggctcggag
      601 gggacgctcg gcgtcgtcac taaggtcatc gtcaacctgg agccaaagcc gggcaagacg
      661 gtgaacctcc tcgccgcatt ccccgatctc gactcggcgg tggacagcgt ggcgaaggtc
      721 atccgcaccg gcatcccggt catctcctgc gagctgctgg acaagatctc ggtaaacatc
      781 accacgaagc acgtcaacac gccgcttccc taccaggaca aagccgacgc ctttttgctc
      841 atccagatag agggcgactc ggaggagcgt ctcgaagacg gctacgagaa ggtgggaaac
      901 ctctgccttc agaacggcgc gctggaggtc ttcgtggcgg agagccggac cgaatccacg
      961 gcgatatgga acatccggca gaatctcgcc gaagccatgc gcgcctacga cccgtactgc
     1021 tcgctcagcg gcgacatggt ggttcccctc tccgccgtcc ccgagatggt ggaggagata
     1081 cgcaaggcgg cgaaggaacg cggcatcgcg gtcggcatcc tgggacacat cgccgacggc
     1141 aacctccacc cgatcatctt caaaccggag tcgatggagc cgatggagtg ggcggagtac
     1201 gcggagacct tctacgacga tttgaccagc gtcgctctcc ggctcggcgg cgtaggaagc
     1261 ggagagcacg gcatcggcta cgtgaagatg cccatcttcc ttcattcaaa gtccgacgaa
     1321 gagctggaga tcatgcgcgg gatcaagcgt tccttcgacc cgcagggcat tctaaacccc
     1381 ggcaagctgc tggggaactg acggcaacag cgggggcggc ctgtacagga cacccccggc
     1441 gcactcata
//
LOCUS       DAOSWW010000799         1442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_80708_length_1442_cov_2.664023, whole genome shotgun sequence.
ACCESSION   DAOSWW010000799 DAOSWW010000000
VERSION     DAOSWW010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80708_length_1442_cov_2.664023"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK442_13155"
     CDS             complement(<1..568)
                     /locus_tag="PK442_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO88644.1"
                     /translation="MKKYAVFLLFISVFGGLILYRRHEIASTTTGVPTTEAIPVRTLS
                     LLPATFEETVSFAANVEPEETAAVVSKVPGRTVLRVHVNEGDYVKKGQKLAEVDDSLL
                     RQQIAQAEAVLGKARTYAATIGAEYKRIAALYQENVVSRQQYERMDGEAKVAVRQVEE
                     ARAGVNQLRIMLGYHTLEAPVSGVVLSRT"
     gene            complement(565..>1442)
                     /locus_tag="PK442_13160"
     CDS             complement(565..>1442)
                     /locus_tag="PK442_13160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO88645.1"
                     /translation="DLRRAEGKLTVAEDVFALTKEHLQHVEALFRNGMTAKNEVLRVQ
                     VSVSEAELNLIRAENAVAVEWKALERAVGVPLCGAFDLPPREESPELFSMPQDAVSVG
                     LAHRPEMEALALAARSASLASKAASAQGGPQIALVGEAYAADEEFFPQKQNDWKVSLL
                     ASWSFFDGGASSAAAREAKAAADEFLFQMEDMKRQIELEISVARLNCESSLQRISTGR
                     TMVESAQEDYRMALRRYTAQVGTNIDVLDSRVALSNARNQLVESVYDSLKARAEMEYA
                     MGVIGNDGLTEERGK"
BASE COUNT          262 a          502 c          384 g          294 t
ORIGIN      
        1 cagtccgcga gagcacaact cccgatacag gcgcctccag cgtatggtag ccgagcatga
       61 tccgaagctg gttcaccccg gcccgggcct cctccacctg gcgcacagca accttcgcct
      121 ctccgtccat cctctcgtac tgctgcctgc tgacgacgtt ctcctgatag agagcggcga
      181 tccttttgta ctccgctccg atcgtcgcgg cgtacgtccg cgccttcccc agcacggcct
      241 cggcctgcgc gatctgctga cgcagaaggc tgtcgtccac ttcggcaagt ttttgtccct
      301 tcttcacata atctccttcg ttcacatgca cccgaagcac cgtgcgccct gggaccttgc
      361 tcacgacagc ggccgtctcc tccggttcaa cgttcgcagc aaaggaaacg gtttcttcga
      421 aggtcgcggg aaggagcgaa agagtacgta caggaatcgc ttccgtcgtt ggaacacccg
      481 tcgtcgtcga ggcgatttcg tgccgccggt agagtatgag gccgccgaag acagagataa
      541 agagcagaaa caccgcgtat tttttcattt cccccgctcc tccgttaatc cgtcgtttcc
      601 gatgacgccc atcgcgtact ccatctccgc cctcgccttg agagagtcgt acacgctctc
      661 cacaagctgg tttcgggcat tggatagggc gacgcgcgag tcgagcacgt cgatattcgt
      721 tcctacctgg gccgtgtaac ggcgcagcgc catcctgtag tcttcctgcg cgctctcgac
      781 catcgtcctg cccgtgctga tccgctgcag ggacgattcg caattcaacc tggcgacgga
      841 gatttcaagt tcgatctggc gtttcatatc ttccatctga aaaagaaact cgtccgctgc
      901 ggccttggcc tcgcgagctg cggctgacga agcgcctccg tcgaagaagc tccaggaagc
      961 cagcagactc accttccaat cgttctgctt ctgcggaaag aattcctcgt ccgccgcata
     1021 cgcttctccc acaagcgcga tctgcggccc tccctgcgcg ctcgccgcct tcgaggcaag
     1081 cgacgccgaa cgcgccgcca acgcaagcgc ttccatctcc gggcgatggg cgagtccgac
     1141 cgaaacggcg tcctgcggca tggagaagag ctccgggctc tcttcgcgcg gcggaagatc
     1201 gaacgcgccg cagagcggga cgccgaccgc tcgctccaac gccttccact ctaccgcaac
     1261 ggcgttctcc gccctaatga ggttcagttc cgcctccgaa acggagacct gcacccgaag
     1321 cacttcgttc ttcgccgtca tcccgttccg gaagagcgcc tcgacatgct ggagatgctc
     1381 ctttgtaagc gcgaacacat cctcggcaac ggtcagcttc ccctcggcgc gccgaaggtc
     1441 gt
//
LOCUS       DAOSWW010000800         1438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_81076_length_1438_cov_3.402748, whole genome shotgun sequence.
ACCESSION   DAOSWW010000800 DAOSWW010000000
VERSION     DAOSWW010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81076_length_1438_cov_3.402748"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1276)
                     /locus_tag="PK442_13165"
     CDS             complement(257..1276)
                     /locus_tag="PK442_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOO88646.1"
                     /translation="MNKQDYKGVMIVVQQMDNRISGVSYELIGKGKELAEALRTEVTA
                     VLLGYEVESLCAALGKYGADRVLMVDDPALETYMTEPYARAMAAIIERFKPEIVLFGA
                     TAIGRDLAPRLSARVHTGLTADCTKLEIDPETRDLMMTRPAFGGNIMATILCSNHRPQ
                     MATVRPGVMQRIAPLESVECPVERVRVDGLAEGKNVEILEIVKKENKRQDIQEAKVLV
                     SGGRGMGGPENFSMLEKLADALGGTVSSSRASVDAGWAEKDRQVGQTGKTVRPNLYIA
                     CGISGAIQHLAGMEESDVIVAINKDPAAPIFDVADFGVVGDALKILPPLTESIKAIRA
                     EKKGA"
BASE COUNT          305 a          452 c          350 g          331 t
ORIGIN      
        1 acccgtgctc gcatacgcgg gcatcgcgct gggcaaggac atggagaccc tcaagaagat
       61 ggggtggaaa atcgtcgtcg tcagcatctt cgtcttcatg ggcacgttca tcggatcggc
      121 tgttatcgcc catctcattc tgaaaatgca gggcataatt tagcccgcga ttcttccgca
      181 aacggtagcg ccggcgttct cgaacgccgg cgctaccgtt tgcgcatacc gccaaatccc
      241 ccgacaagac ggtatgctat gctccttttt tctccgccct gatagccttg atactctctg
      301 ttagaggggg aagaatcttc agcgcgtcgc caactacgcc gaagtccgca acgtcaaaga
      361 taggcgctgc gggatccttg ttaatcgcca cgatcacatc ggactcctcc ataccggcca
      421 gatgctgaat cgccccggaa atgccgcatg cgatataaag attgggacgt accgtctttc
      481 ccgtttgccc cacctgacga tccttctccg cccatccggc gtcgacgctt gcacgggagg
      541 aggaaactgt cccgccgagc gcgtccgcca gtttttcgag catggaaaag ttttcaggac
      601 ctcccattcc gcgacctccg gaaacgagaa ctttcgcctc ctggatgtcc tgtcttttat
      661 tctccttttt cacgatctcc aaaatttcaa cattctttcc ctcagccagc ccgtctacgc
      721 gaacgcgttc cacaggacat tctacactct ccaacggagc aatgcgctgc atgacgcccg
      781 gacggacggt cgccatctga ggccggtggt tcgagcaaag aatcgtcgcc atgatattac
      841 cgccgaaggc cgggcgggtc atcataagat cgcgcgtctc cgggtcgatt tccagtttgg
      901 tacaatccgc ggtcagaccg gtatgaaccc gcgccgataa gcgaggtgcg agatcgcgcc
      961 cgatagcggt ggcgccgaac agaacgattt cgggtttgaa acgttctatg atggcggcca
     1021 tcgcacgcgc gtacggctcc gtcatatacg tctccagcgc gggatcgtcg accataagaa
     1081 ctctgtccgc tccatatttc cccaatgcgg cgcacagcga ctccacttca taccccagca
     1141 gaacggcagt aacctccgtc cgcaatgctt cggccaactc cttgcccttt cctatcagtt
     1201 cgtaggagac cccggagatc cgattatcca tctgctgcac cacaatcata acgcctttat
     1261 aatcctgttt gttcatcggg ctctcacctc tccatcacag aatgaacttt tcctgcagac
     1321 gatccgtgat ccagtcgacg gcgtcgtgag cgtcgagcgc aacgactgta ccttttccct
     1381 ttaccgcctt tgtaaaagat ttgacgactc gcgtaggaga gcctttcaaa ccgatatt
//
LOCUS       DAOSWW010000801         1437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_81161_length_1437_cov_3.986252, whole genome shotgun sequence.
ACCESSION   DAOSWW010000801 DAOSWW010000000
VERSION     DAOSWW010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81161_length_1437_cov_3.986252"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PK442_13170"
     CDS             complement(<1..287)
                     /locus_tag="PK442_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88647.1"
                     /translation="MLPTPSSKEGEVAEDMSPKKSADETLFEDVPKLDDPRLYLNREI
                     NWIDFNSKVLEEAMDPSTPMLEQLKFLSIFFNNLDEFFMVRVSALLEQYRGG"
     gene            complement(474..1277)
                     /locus_tag="PK442_13175"
     CDS             complement(474..1277)
                     /locus_tag="PK442_13175"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOO88648.1"
                     /translation="MSSLKYRSVFLSDFHLGTRWCRAKSLVSFLGSMECEKLYLIGDI
                     IDGWKLKRSPGWPNSHNSVIRKILKMSKKTPVLYIPGNHDDFMKEFNGYSFGGVKICE
                     NAIHTSPEGKRFLVVHGHEFDVAVMYKPWLAFLGDRAYDISLHLNTGLNAIRSFLGFG
                     YWSLSEFLKRKVKDAVKFIGNYEECVAKAAGKYGVDGVICGHIHNPAIRRFKGIDYFN
                     CGDWVESCSALVEHLDGAMEIIRWFDKKSKKDEENVVFLEERSAVPAAD"
BASE COUNT          315 a          403 c          383 g          336 t
ORIGIN      
        1 ccgccccggt actgctccag caacgcggag acacgcacca taaagaactc gtcgaggttg
       61 ttgaagaaga tggagagaaa cttgagctgc tccagcatcg gcgtggacgg gtccatcgcc
      121 tcttcgagca ccttcgagtt gaaatcgatc cagttgatct cccggttcag ataaagacgg
      181 ggatcgtcga gcttcggaac atcctcgaac aacgtttcgt ccgcactctt cttcgggctc
      241 atatcctccg cgacctctcc ttctttcgaa gatggcgtgg ggagcatcgt aacgacctcc
      301 tcaataacat tatacacgtg catttcaacg ctcatggtac atgagcgcgg ttggttacgc
      361 cacagcggaa tgagagaacc gaaaaactgg tacacttccg taatattgga gagaaaaaac
      421 gtccgcgcgg gatcggcgga cggcgtgtga acgcaggtat agggcgagag gactcaatct
      481 gctgcgggaa ctgcgctgcg ctcttccaga aagacgacgt tttcctcatc ctttttcgat
      541 tttttgtcga accagcggat gatctccatc gcgccgtcga ggtgctccac gagggcggag
      601 cagctttcga cccagtcgcc gcagttgaag tagtcgattc ccttgaaacg gcgaatcgcc
      661 ggattgtgaa tatgtccgca gatgacaccg tcgacgccgt acttccccgc ggctttcgcg
      721 acgcactctt cgtagttgcc gatgaacttc acggcgtcct tgacctttcg cttcagaaac
      781 tcggagagcg accagtaccc gaagccgagg aaggaacgaa tggcgttcaa ccccgtgttc
      841 agatgcagag agatatcgta ggcacggtct ccgaggaagg cgagccaggg cttgtacatc
      901 accgcgacgt cgaactcatg tccgtggacc accaggaatc gtttcccttc gggggaagtg
      961 tggatcgcgt tctcgcaaat cttcacgccg ccgaacgagt agccgttgaa ctccttcata
     1021 aagtcgtcgt ggttgccggg gatgtagagc accggcgttt tcttcgacat cttcagtatc
     1081 ttccggatta ccgagttgtg cgagttcggc catccggggc ttctcttcag cttccatccg
     1141 tcgatgatgt cgccgatcag gtagagcttc tcgcactcca tcgacccgag gaatgacacc
     1201 agactcttcg ctctgcacca gcgcgttccg aggtggaaat cggagaggaa gacggaccgg
     1261 tatttcagag aactcatccc tttcttctct tttcttgcgt catagcataa ctcctttcgg
     1321 taacggaagt tccgtgcgaa tgatacgaaa atgtatcggt tgtatctccc tgcgtcgact
     1381 ccaaccgtat aagtatacct cttttaaagc cgctcagtcg ttttccgtcg agccggg
//
LOCUS       DAOSWW010000802         1435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_81351_length_1435_cov_4.100725, whole genome shotgun sequence.
ACCESSION   DAOSWW010000802 DAOSWW010000000
VERSION     DAOSWW010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81351_length_1435_cov_4.100725"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PK442_13180"
     CDS             <1..872
                     /locus_tag="PK442_13180"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOO88649.1"
                     /translation="PYYSLRRVWRVLSLVAPSKNYSPWVEDGFTKDYPFSVAPDKKLT
                     VRDVMRLHRDHYEGTEFDLTKGAAAGPFGYPDRFYGPYDGAGDVGDPKRVLEGAWERP
                     LSVTYCGYVFVAQARSWLPSPVGGRMWLGLDKPSDTVFIPFHIGVNRLPSSVEVCDTT
                     KFSRESAWWTFNFLANYAGLKYSYMHKEIADMQDQLENGFLETLEIVERKAVDLHRTN
                     PAGARAYLTEFAERNTAVTLKEWWNLAEHLIVKYNDGLLTEGGKIGRPLGYPKEWLKT
                     TSWPEGPTSYRKR"
     gene            complement(988..>1435)
                     /locus_tag="PK442_13185"
     CDS             complement(988..>1435)
                     /locus_tag="PK442_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88650.1"
                     /translation="HRDVCVSYAIDVGGLTAGSLEDALKGLLGGLQSGMEKADFRDIS
                     FTLKGTTEKPGVSNLKIAPGAQQPGQPAPTGQPAEPQDPKKKLEQMLIEQIVKPSKPA
                     QPEQPQQMQPAQPDAPASSPTPAPTPTPEKKPEDIIKDKILESIFK"
BASE COUNT          289 a          414 c          433 g          299 t
ORIGIN      
        1 atccgtacta ctctcttaga agggtctggc gggtcctctc gctggtcgct ccctcgaaga
       61 actactctcc gtgggtcgag gacggtttta cgaaagacta ccccttctcc gtggcgcctg
      121 ataaaaaact tacggtgcgc gacgtgatgc gcctccaccg cgatcactac gagggaacgg
      181 agttcgacct caccaaggga gcggcggccg gaccgttcgg ctaccccgac aggttctatg
      241 gtccgtacga cggcgccggc gacgtcgggg atccgaagcg cgtgctggaa ggcgcatggg
      301 aacgtccgct ttctgtgacg tactgcggct acgtgttcgt cgcccaagcc aggagttggc
      361 tcccctcgcc cgtcgggggg aggatgtggc tcggtctcga caagccgtcc gacacggtct
      421 tcatcccgtt ccacatcggc gtcaaccgtc ttccgagctc ggtcgaagtc tgcgatacga
      481 cgaaattttc ccgcgagagc gcgtggtgga cattcaactt cctcgcaaac tatgccgggc
      541 tcaagtacag ctacatgcac aaggagatcg ccgatatgca ggaccagctt gaaaacggtt
      601 tcctggagac gctggagatc gtcgaacgca aagcagtcga cctgcacagg acgaacccgg
      661 ccggagcgag ggcctacctc accgaattcg ccgaacggaa cacggcggtt acgctgaaag
      721 agtggtggaa tctggccgag catctcatcg tgaagtacaa tgacgggctg ctgacggagg
      781 gaggaaagat cggccggcct ttgggctatc cgaaagagtg gctgaagaca acctcgtggc
      841 cggagggacc aacgagctac cggaagagat aagcacattc caactgatcc cgctttgagg
      901 gatacgaatc tccatcggga gcgaaaaaga gttgtggtca tacaaaacag gggaggcgta
      961 cgcctcccct gttcgttctt tccgctatta tttgaatatg gattcgagaa ttttgtcctt
     1021 gataatatct tccggtttct tctccggagt cggcgtcggc gcaggcgtag gagacgaagc
     1081 gggcgcgtcg ggctgcgcag gctgcatctg ctgaggctgc tccggttgcg caggctttga
     1141 aggcttcaca atctgctcta tgagcatctg ttccagcttc tttttcggat cctgcggctc
     1201 ggccggctgt cccgtaggag cgggctgccc gggctgttgc gcacccgggg cgatcttcaa
     1261 attggagacg ccgggtttct ccgtcgttcc tttcaaagta aaggaaatat ctctgaaatc
     1321 ggccttctcc ataccgcttt gcaggccgcc cagtaaccct ttcagcgcat cttccagcga
     1381 accggcggtc agcccgccga cgtcgatggc gtagctcacg cagacgtcgc ggtgg
//
LOCUS       DAOSWW010000803         1434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_81492_length_1434_cov_2.849891, whole genome shotgun sequence.
ACCESSION   DAOSWW010000803 DAOSWW010000000
VERSION     DAOSWW010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81492_length_1434_cov_2.849891"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PK442_13190"
     CDS             <1..517
                     /locus_tag="PK442_13190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO88651.1"
                     /translation="LSGWSGVRILTSPLSRALETSAIIAAACGAVSEPQEGLSEMGFG
                     AWEGRSIFETETSDPQAFREWKNNPFKFVPPAGEGFPEIQNRLLPVLDEALSSVQERI
                     LLISHGGIIRAALSLLLDLSQESVWRMKLSNCSVTAVESGKRGKSLLFLNDDIHTVLP
                     AVFLPNLPFPS"
     gene            578..1222
                     /locus_tag="PK442_13195"
     CDS             578..1222
                     /locus_tag="PK442_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO88652.1"
                     /translation="MTGKATSAEKRDASLPQESAMVLPDTAGGSPEAMDEATLWRRCA
                     EEHDEDARDSLIVSYRPLVFWLAKKFHVHPSSYQDLIQEGMLALIRAVDKFEPERHLK
                     FTTYAFYRIKGQMINFLQRSEKRAPVPVDDEFLMPEDPFLPETFETMLALSEELRRLP
                     AKEGEVLQAILMDGREAREVAQERGIDISHVYRLKRNALTKLRKWLGAEATNEV"
BASE COUNT          332 a          360 c          418 g          324 t
ORIGIN      
        1 cctcagcggc tggagcgggg tgagaatact cacgagtccg ctctccaggg ctcttgagac
       61 atccgccatc atcgccgcag cctgcggggc agtctcggaa ccgcaggaag ggctctcgga
      121 gatgggattc ggcgcatggg aagggcgctc gatctttgaa acggaaactt cggatccgca
      181 ggccttcaga gagtggaaga acaatccctt caaatttgtc cctccggccg gggagggttt
      241 tccggagatt caaaatcgcc ttcttccggt gctcgatgaa gccctctcct ctgttcagga
      301 gcgaatcctt ctcataagcc acggagggat catccgcgcc gctctttccc tcctgcttga
      361 tctgagccag gagtcggtgt ggaggatgaa actgtccaac tgttctgtga cggctgttga
      421 atcgggaaag cgcggcaaat cccttctctt tctcaatgac gacattcata ctgttttgcc
      481 ggctgtattt ttaccgaatc tccccttccc ctcttagaca agaggggctc ttgctggtag
      541 aatttactgg aattcgatac gagggagtgt gtatgccgtg accgggaaag caacatctgc
      601 agaaaaacga gacgcaagtc tccctcaaga gagcgcgatg gttcttcccg atacagccgg
      661 aggctcccct gaagcaatgg atgaagcgac gttgtggagg agatgtgcgg aagagcacga
      721 tgaagatgcg agggattccc tgatcgtttc ctatcgtcct ctggtattct ggctcgcaaa
      781 gaaatttcac gttcacccct cgtcgtacca ggatctgatt caggagggga tgcttgccct
      841 cattcgtgcg gtggacaagt tcgagccgga acgtcatctc aaatttacca cctatgcgtt
      901 ttaccggatt aaaggacaga tgatcaactt cctgcagcgc tccgaaaaaa gggcccccgt
      961 tcctgtggac gatgaattcc ttatgccgga ggaccctttc cttcctgaaa cgttcgagac
     1021 gatgcttgct ctttcggaag agttacgacg gctcccggca aaggaggggg aggtgctgca
     1081 ggcaattctt atggatggac gggaggcaag agaggtagcg caggagcgcg gaatcgatat
     1141 cagtcacgtc tatcgtctga agaggaatgc gctgacgaaa cttcgcaaat ggcttggcgc
     1201 cgaggccaca aacgaggtct aaaacgggat aagagaatac agtgaacgcc cacgcgcgtt
     1261 tttttgcttg aaagcgtgga gtacattccc gggaggactt tccatggaga agaagatcgc
     1321 tcgaatttat agatggcttg aacgatgcgc gaaggcatgt tccgcaggtt cttggaacag
     1381 cgcgctgctt gatatggagt gcgcgaaagc cgagctggaa gatgtccgga cgga
//
LOCUS       DAOSWW010000804         1432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_81681_length_1432_cov_2.235294, whole genome shotgun sequence.
ACCESSION   DAOSWW010000804 DAOSWW010000000
VERSION     DAOSWW010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81681_length_1432_cov_2.235294"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PK442_13200"
     CDS             <1..604
                     /locus_tag="PK442_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88653.1"
                     /translation="SILMGVASPTEAAAFGAGGALFIGLIKRRLTWEIVYSSCFETLS
                     VSSMVGWTMIGAGAFGSVFSGLGGNTLIANIAMNMPGGPNMVFFVSAVFIFILGMFLE
                     PGAIIFLAVPIVAPILARLGFDPLWVGLVFNVLLQTAYLSPPFGFSLFYLKGCTPEDI
                     DIVDIYKASVPFLLLQIVCITLIFLYPSIVLWLPRYVLGM"
     gene            722..>1432
                     /locus_tag="PK442_13205"
     CDS             722..>1432
                     /locus_tag="PK442_13205"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88654.1"
                     /translation="MKKTLAVLLTIAILCTGVSAAFAAKVNWRLVTHASPGTEQQRIA
                     EVFCETVKTLSGGEFVIEPYAAGVLFPVFESFDFLANGVIDASMVYSAYWTGKDTGYT
                     LTTLPGCPLSTYAEGAYLVEQLFPYFEKLYAKYGITYLGHAMVSPINEQLISVVPINT
                     LEDIKGKKIRTSGFGARYYSALGATTVSLSGPEIYTALQTKNLDAAEWTFWDENMRMG
                     LHEVANYVLDPALQNGTCE"
BASE COUNT          312 a          328 c          386 g          406 t
ORIGIN      
        1 ctcgattctc atgggagtag ccagcccgac cgaagccgcg gcattcggcg cgggaggcgc
       61 tctctttatt ggcctcatca agcggcgtct gacatgggaa atcgtgtatt catcttgttt
      121 tgaaacgctt tctgtttcgt cgatggttgg atggacgatg ataggagcgg gagcattcgg
      181 atccgttttt tcgggtttgg gcggcaacac attgatcgcc aatatcgcca tgaacatgcc
      241 gggtggaccg aacatggtct ttttcgtatc cgccgttttc atattcatcc tcgggatgtt
      301 tttggagccc ggtgcgatta ttttccttgc agttcctatt gttgctccaa ttctggcgcg
      361 actcggtttt gatccgctat gggttggcct tgttttcaat gttcttcttc agacggcata
      421 tttatctcct ccgtttggtt tcagcctttt ttatctcaag gggtgtacgc cggaagatat
      481 tgatattgtt gatatataca aggccagcgt accttttttg ctgctccaga ttgtatgcat
      541 cacgctgata ttcctctatc cgtccatagt tctatggctt ccgaggtatg ttcttggtat
      601 gtagatgagg aaaggaggtg gtatttcagg gaggtttgtt cagagagagt gaggagaagt
      661 tgtggaactg aagctgaaca atcaaaagca aaacgattcc agaaaaggag gtatttgaag
      721 gatgaaaaag actctcgcgg tactcttaac catagctata ctatgcacag gtgtaagcgc
      781 ggcttttgcg gcgaaagtga actggagatt agtgacgcat gccagtcccg gaacggaaca
      841 gcagcgaata gcggaggttt tttgcgaaac ggtgaaaacg ctctccggcg gtgagttcgt
      901 tatcgaaccg tacgcggccg gggttctttt ccccgttttc gaatcctttg atttcttggc
      961 gaacggcgtt atcgatgctt cgatggtgta cagcgcctac tggacgggga aggataccgg
     1021 ttacaccctg acgactctgc ccggatgtcc tctgagtacc tatgcggaag gcgcatatct
     1081 cgtcgaacaa cttttcccct attttgaaaa gttgtatgcg aaatatggca taacatacct
     1141 tggacatgct atggtcagcc ccatcaatga acagcttatc tccgtcgttc cgataaacac
     1201 tctggaggat atcaagggga agaagatccg tacgtccggt tttggcgccc gctattacag
     1261 cgcccttggt gcgactaccg tttcgctctc cggcccggaa atttatacgg ctctgcagac
     1321 gaagaacctt gatgccgccg agtggacatt ctgggatgag aatatgcgta tgggcctcca
     1381 cgaggttgcc aactatgttc ttgaccctgc gttgcagaac ggtacgtgcg ag
//
LOCUS       DAOSWW010000805         1431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_81734_length_1431_cov_3.436047, whole genome shotgun sequence.
ACCESSION   DAOSWW010000805 DAOSWW010000000
VERSION     DAOSWW010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81734_length_1431_cov_3.436047"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1213)
                     /locus_tag="PK442_13210"
     CDS             complement(35..1213)
                     /locus_tag="PK442_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacylase"
                     /protein_id="HOO88655.1"
                     /translation="MFDYEKFKTPRKVVLLLLALALAWLGGREFTELRTMKETVVVSD
                     AFTKMVMLSDYFPPIKGTGVDTPVYIYDSGVPGGSMFYLGGTHPYEPATMLSAYVMME
                     NLKVEKGRVFICPHANMSASTIGMLGNAYPKFLHVETAFGPKPYRIGDRNTSPLDQWP
                     DPFTYVHYPSGQNLDYTDARNLNRTYPGRPDGNLTEQLTFAIMELNRKEKITLFVDAH
                     EASLMYPVVSTYVAHDRALDMAMMASMMLSATEFPMKCEASPKNLRGLSHRDAGDFCD
                     SLVILMETPEPFIDRVAGKITEKLMMDGKDEFLQTAAEHGLLFTDYDISVGCPMWDRV
                     GRHLSGALAMIQQMNDFLMEPEKELQLSFPNYSDLKEHGLGHFLNDPAKADPAKVFLN
                     "
     gene            complement(1225..>1431)
                     /locus_tag="PK442_13215"
     CDS             complement(1225..>1431)
                     /locus_tag="PK442_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88656.1"
                     /translation="RKRGVWIMEEFLRVSNEIIHQIYFILAGLCGLVLLRGLFSRNTR
                     KTIVYDIVYAYTIIPFLLRALHIK"
BASE COUNT          267 a          408 c          456 g          300 t
ORIGIN      
        1 ctcccgccgg gagaagcctt acaggatggt accgtcagtt caaaaagacc tttgccggat
       61 ccgctttcgc aggatcgttc aggaaatggc cgagtccgtg ttccttcaag tcggagtagt
      121 tcggaaagga gagctgcaac tccttctccg gctccatcag gaagtcgttc atctgctgga
      181 tcattgccaa cgctccggag agatggcgcc ctaccctgtc ccacatgggg cagccgacgg
      241 agatgtcgta gtcggtgaac agcaggccgt gctccgccgc ggtctggagg aactcgtcct
      301 ttccgtccat catcagcttt tcggtgattt tgcccgccac tctgtcgatg aacggttcgg
      361 gcgtctccat caggatgacc agcgagtcgc agaaatcgcc cgcgtcgcgg tggctcaagc
      421 cccgcaggtt cttgggggag gcctcgcact tcatcgggaa ctcggtggcc gacagcatca
      481 tggacgccat catcgccatg tcgagcgccc ggtcgtgggc cacgtaggtg gagaccacgg
      541 ggtacatgag ggaagcctcg tgcgcgtcga cgaagagggt gatcttctct ttccggttca
      601 gctccatgat ggcgaaggtg agctgctccg tcagattgcc gtccggccgg ccggggtagg
      661 ttctgttgag gttgcgcgcg tcggtgtagt caaggttctg ccccgacggg tagtggacgt
      721 acgtgaacgg gtcgggccac tggtcaaggg gcgaggtgtt cctgtcgccg attctgtacg
      781 gcttggggcc gaacgccgtc tccacgtgga ggaacttcgg gtaggcgttc cccagcattc
      841 cgatggtgga ggcgctcatg ttggcgtggg ggcagatgaa gaccctcccc ttctccacct
      901 tgaggttctc catcatgacg taggcggaga gcatcgtggc gggctcgtag gggtgcgtcc
      961 cgccgaggta gaacatcgaa ccgccgggca cgcccgagtc gtagatgtag accggcgtgt
     1021 ccacccccgt ccccttgatg ggagggaaat agtcgctgag catgaccatt ttggtgaaag
     1081 cgtccgagac gacgaccgtc tccttcatcg tgcgcagctc ggtgaactcc ctgccgccca
     1141 gccaggcaag cgccagcgcg agcagcagca aaacgacttt ccggggcgtt ttgaattttt
     1201 cgtagtcgaa cattcgccgt caccctactt tatgtgcagc gcgcgcagca ggaagggaat
     1261 gatcgtgtag gcgtagacga tgtcgtacac gatggtcttg cgcgtgttgc gggagaacag
     1321 cccccggagg aggacaaggc cgcacagtcc ggccaggatg aaatagatct gatggatgat
     1381 ctcgttggat acgcgaagaa actcttccat aatccagact cctctctttc t
//
LOCUS       DAOSWW010000806         1431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_81750_length_1431_cov_2.917151, whole genome shotgun sequence.
ACCESSION   DAOSWW010000806 DAOSWW010000000
VERSION     DAOSWW010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81750_length_1431_cov_2.917151"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1431)
                     /locus_tag="PK442_13220"
     CDS             complement(<1..>1431)
                     /locus_tag="PK442_13220"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOO88657.1"
                     /translation="VRETDAFLREELLRTTRVLAETIDIRDVRRLTGSELDLGSPQYE
                     SFKNYFRSVLQMYPRSRFLTLVGRRYDGKVFFFVDSEPPGSEDESPPGEVYDAVDPGF
                     LPAFDEARAVVVGPVVDQWGAWISAWVPLVERGSDRVSAALALDISGEEWQRTLEKAK
                     RLPALTSLFLALLLFAGRWMLQQRTKTGGGAFGRFRYLECLLTLIFGLALTGSVAWMV
                     HERESRSHAQSFSIVAEAHSVKMAGMLYRLRNFVLEGTARFIEALPSLEYESFRRYSQ
                     HLSSEPEIDALMWSPRVLAEERAEFESTERDEWRRALEIREQNGAGAYVRAEERAEYV
                     PVLFRSPMTGSMFPVGEDLLSHPEVSEALRTAVNTRRPAAAFVRVQYGKEDGSALWVC
                     YPIFRRGFNGALRGVVSALFLPSFMPDEVMGGSGDAGMLSFEALSPEAPPRSLAQWEL
                     EGRPEYRFIRPLYAFDGTFAAVLRPGA"
BASE COUNT          287 a          481 c          419 g          244 t
ORIGIN      
        1 ggctccgggg cgcagcaccg cagcgaacgt gccgtcgaac gcgtacaggg gacgtatgaa
       61 gcggtattcc ggtctccctt cgagctccca ctgggcaagc gagcgcgggg gcgcctcggg
      121 agagagcgcc tcgaagctca gcatcccggc gtcgccgctc ccgcccatga cttcatccgg
      181 catgaaagac ggcaaaaaga gcgccgacac gacgcccctg agggcgccgt tgaagcctcg
      241 acggaagatt gggtagcaga cccagagcgc ggagccgtct tcctttccgt actggacccg
      301 tacaaaagcg gcggccggtc ttctggtatt cacggctgtc ctgagagctt ccgatacctc
      361 cgggtgtgaa agaagatcct cccccacagg gaacatgctt cccgtcatcg gggaacggaa
      421 gagcaccgga acgtactccg cccgttcctc cgcgcggacg tacgccccgg cgccgttctg
      481 ctcgcgtatc tccagtgcgc gccgccattc gtcccgctcg gtgctttcaa actccgcccg
      541 ctcttccgcg aggacccgag gactccacat gagcgcgtcg atctccggct ccgacgacag
      601 atgctgtgaa tacctcctga acgactcgta ctccagcgaa gggagcgcct cgataaaccg
      661 cgcggttccc tcgaggacga aattgcggag tctgtagagc attccggcca tcttgacgga
      721 gtgcgcttcc gcgacgatcg aaaaactctg cgcgtgggag cggctttcac gttcgtgcac
      781 catccaggcc acgctccccg tcagggcaag accgaagatc aatgtcagaa ggcactcgag
      841 atacctgaat ctcccgaacg ctcctcctcc cgttttggtc cgctgctgca gcatccagcg
      901 tccggcgaaa agaagcaacg caagaaagag cgacgtcagc gcgggaaggc gcttggcttt
      961 ctccagggtc cgttgccatt cctctcccga gatatccaac gccagggcgg cgctcaccct
     1021 gtcggaaccg cgctcgacca ggggaaccca cgccgaaatc catgcccccc actggtccac
     1081 gaccgggccg acgacgacgg cccttgcctc gtcgaatgcg ggcagaaacc ccgggtcgac
     1141 tgcgtcgtac acttctccgg ggggcgattc gtcctccgaa cccggcggtt ccgagtccac
     1201 aaaaaagaat actttgccgt cgtagcgcct tccgacgagc gtgaggaaac ggctccgcgg
     1261 gtacatctgc aagaccgagc ggaaatagtt cttgaaactt tcatactggg gcgaccctaa
     1321 atccagttcg ctccccgtaa ggcggcgcac gtcgcggata tcgatggtct cggcaagaac
     1381 gcgtgtagta cgcagcagct cttcgcggag aaacgcatcg gtctcgcgca c
//
LOCUS       DAOSWW010000807         1425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_82341_length_1425_cov_3.435036, whole genome shotgun sequence.
ACCESSION   DAOSWW010000807 DAOSWW010000000
VERSION     DAOSWW010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82341_length_1425_cov_3.435036"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1425
                     /locus_tag="PK442_13225"
     CDS             <1..>1425
                     /locus_tag="PK442_13225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO88658.1"
                     /translation="QGEAAASLALRILAGERADSIPVVLESPNRYVFNAAMLQKFAIP
                     EALLPPDSQIVYRTAASLLSNWETLSRRLFFAYDLFAENTTPMLLIDPDTGIILDGNH
                     SARGFYGYPELKGMNIAQINTLSPEEVSREMERARSRNNTSFDFKHRLADGSVRDVGV
                     HSSPVFIDGREILLSLVIDETERVTAQEALGQRNRWIVALVVSALLLQSVVLLQLLRS
                     AAQRKKAEKALRESERKLSALFASMTEVVALHDLVFDEEGRPANYRITGCNAAFTKSI
                     GIPEEAAVGRLATEVYGTEKAPYLHEYGKVAMTGEPSTFETYFAPLDKHFFISVVSPG
                     ENSFATVTTDITSIKKAEQLIEAKNKELEQVVYVASHDLRSPLVNVDGYSRELEYSVE
                     DLLRALDDESLAPPERERAIRALLPDMTDSLRRIRGSAKQMDALLRGLLKLSRIGRAA
                     LSVGSVDMNELMAELSSSLQFQVR"
BASE COUNT          286 a          444 c          425 g          270 t
ORIGIN      
        1 cccagggcga ggccgcggcg tcgttggcgc ttcgcattct cgccggagag cgcgcggact
       61 ccataccggt agtgctcgaa agtccgaacc gctacgtctt caacgccgcc atgcttcaga
      121 aattcgcaat ccccgaggcg ttgcttcccc cggattcgca gattgtctac cgcactgccg
      181 cgagcctcct ttcgaactgg gagacgctgt cccgaaggct cttcttcgcc tacgacctct
      241 tcgcggagaa caccacgccc atgctgctca tcgacccgga tacggggatc atactcgacg
      301 gaaaccattc cgcccgcggt ttttacggct atccggagct gaaggggatg aatatagcgc
      361 agatcaacac gctctcccct gaggaggttt ccagggagat ggagagggcg cgcagccgga
      421 acaacacttc ctttgatttc aagcatcgcc tggccgacgg aagcgttcgc gacgtcggcg
      481 tgcactcctc gccggtgttc atcgatggcc gggaaatctt actctccctg gttattgacg
      541 agaccgagag ggtgactgcg caagaagcgc tggggcagcg aaaccgctgg atagtcgcgc
      601 ttgtcgtttc cgccctgctg cttcagtccg tcgtgctgct gcagctgctg cgaagcgccg
      661 cgcaaagaaa gaaggcggaa aaggcgcttc gcgagagcga acggaaactc agcgcgctct
      721 tcgcctcgat gacggaggtc gtcgccctcc acgacctggt cttcgacgag gaaggccgtc
      781 ctgcgaacta ccggattacc gggtgcaacg ccgcgttcac aaagtcaatc ggaatcccgg
      841 aggaagcggc cgtcggacgc ctggcgaccg aggtctacgg tacggagaag gctccgtatt
      901 tgcatgagta cggcaaggtc gccatgacgg gagaaccctc aactttcgag acgtacttcg
      961 cccctctgga caagcacttc ttcatctccg tcgtctctcc gggcgaaaac agcttcgcca
     1021 ccgtcacgac cgacatcacc tcgatcaaaa aggcggagca gctcatcgaa gcgaagaaca
     1081 aggaactgga gcaggtcgtc tacgtggcgt cgcacgacct tcgttccccg ctggtcaatg
     1141 tggacggata cagtcgcgag ctggagtact ccgtggagga tcttctgcgc gcgctggacg
     1201 acgaatctct cgctcctccg gaacgggagc gcgcgatacg cgccctgctc cccgacatga
     1261 ccgactctct gcgccgtatc agaggaagcg cgaagcagat ggacgcgctc cttcgaggcc
     1321 tcttgaagct ttcgcgcatc gggcgggcgg cgctcagcgt cggatccgtc gatatgaacg
     1381 agctgatggc ggaattgagt tcctctctgc aatttcaggt cagag
//
LOCUS       DAOSWW010000808         1425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_82350_length_1425_cov_3.180292, whole genome shotgun sequence.
ACCESSION   DAOSWW010000808 DAOSWW010000000
VERSION     DAOSWW010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82350_length_1425_cov_3.180292"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK442_13230"
     CDS             <1..214
                     /locus_tag="PK442_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOO88659.1"
                     /translation="KRVRMLVEDDGAGFSSKAPKHWGKWGGMGLFSIREKLHSVGGEI
                     RVFSEKGKGSTVALVAPLMFEGGKTS"
     gene            211..516
                     /locus_tag="PK442_13235"
     CDS             211..516
                     /locus_tag="PK442_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO88660.1"
                     /translation="MKIRVLMIDDHTLFLAGLEDILAREDDIEVVGVAGSCREGVSLA
                     MEKRPDVIVLDLAMPGTSGVEAARILAEALPGVRMAALTMHLDRQMVFEALKSGMTG"
     gene            520..861
                     /locus_tag="PK442_13240"
     CDS             520..861
                     /locus_tag="PK442_13240"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO88661.1"
                     /translation="MKEATPEEFLFALRVVARGETWLSPKVAAIVANDFVRGAAQEAP
                     SLPVLSDREREVLHLLVKGAGTREIASRLHISKSTVDTHRRNILDKLGCENVTCLTRY
                     ALRHGLVDLDE"
     gene            925..1257
                     /locus_tag="PK442_13245"
     CDS             925..1257
                     /locus_tag="PK442_13245"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO88662.1"
                     /translation="MAYSVEYFNDRVRAEIEAWPVGILADYARILEMLMEFSPEVGMP
                     YSRPMGDGLFELRPRGREGIGRALYCYMRGRRLLILHAFVKKTGSTPQKDLNRARDRL
                     KEVRHDWM"
     gene            1244..>1425
                     /locus_tag="PK442_13250"
     CDS             1244..>1425
                     /locus_tag="PK442_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88663.1"
                     /translation="MTGCEYKPVPHNHDEFLAAMSEREGFASAYAALEDEYRFVRELL
                     AARIRAGLTQEEVAES"
BASE COUNT          281 a          397 c          465 g          282 t
ORIGIN      
        1 caagcgcgtc cgcatgctcg tcgaggacga cggcgcgggg ttctcctcca aggcgccgaa
       61 gcactggggg aagtggggcg gcatgggcct tttcagcatc cgtgagaaac ttcactccgt
      121 cggcggagag attcgcgtct tctcggagaa ggggaaaggg agcaccgtcg cgctcgtcgc
      181 gccgctcatg ttcgagggag gaaagacgtc atgaagatcc gcgtgctgat gatcgacgat
      241 catacgctct tcctggccgg gttggaggac attctcgccc gcgaggacga catcgaagtg
      301 gtcggcgtcg ccggaagttg cagggagggc gtgtcgctgg cgatggagaa gcgccccgac
      361 gtgatcgtcc tcgacctggc gatgccgggg accagcggcg tggaggcggc gcgcatcctc
      421 gccgaggcgc tgccgggcgt ccgcatggcg gcgctcacga tgcacctcga ccgccagatg
      481 gtcttcgagg cgctcaaaag cggcatgacc ggctagattt tgaaagaggc cactccggag
      541 gagttcctct tcgcgctccg cgtggtggcg cgcggggaga cctggctcag cccgaaggtg
      601 gccgccatcg tggcgaacga cttcgtccgc ggcgccgctc aggaagcgcc gagccttcct
      661 gtcctctcgg atcgcgagcg cgaagtactc catctcctcg tcaaaggggc gggaacgcgt
      721 gagatagcgt ctcgtctgca catcagcaag agcaccgtgg acacccaccg gcgcaacatc
      781 ctcgataagc tgggctgcga aaacgtcacc tgcctcaccc gctacgccct gcgccacggc
      841 ctggtcgacc tggacgagtg aggggcaagg tttttcaagt cactctgcat tgacgtgtgt
      901 tctcatattt ggtaacatgc ggatatggct tattccgtcg aatacttcaa tgatcgagtc
      961 cgtgcagaga tagaagcgtg gcccgtcggt attcttgccg actatgcgcg gattcttgag
     1021 atgcttatgg agttcagtcc ggaagtgggt atgccgtatt cccgtccgat gggagacggt
     1081 cttttcgaac tgcgcccccg aggacgcgaa gggatcggac gtgcgttgta ctgctatatg
     1141 agaggaaggc gtctgctcat actccacgcc tttgtaaaga aaacgggctc caccccgcag
     1201 aaggatttga atagagcccg cgatagattg aaggaggtgc gtcatgactg gatgtgaata
     1261 taaaccggtt cctcataatc acgatgagtt tctggccgcg atgtcggaaa gagaaggttt
     1321 tgcctccgcg tatgccgcgc tggaggatga ataccgattt gtccgcgaac ttttggcggc
     1381 caggatacgc gcaggtctca cacaagaaga ggttgcggag tccat
//
LOCUS       DAOSWW010000809         1424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_82456_length_1424_cov_3.265888, whole genome shotgun sequence.
ACCESSION   DAOSWW010000809 DAOSWW010000000
VERSION     DAOSWW010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82456_length_1424_cov_3.265888"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PK442_13255"
     CDS             complement(<1..1397)
                     /locus_tag="PK442_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO88664.1"
                     /translation="MEELRELRERLLDAEGKLLETERFLADLIENSGSLMVIKSVDGV
                     YTFVNRRWEECTGLPRADVLGRTDAELFSPEAAEHFRRNDSEVLRRGELVEFEEVFDR
                     PDGRHFFLSGKFPVHGEDGTITGLCAVITDITDRKRAEEKLRISRERYELAMAATNDG
                     LWDWNLVTDKVYFDPRYYTMAGYEPGEFPSSFDEWAKRLHPDDHPLCNRALQSYFSGK
                     TPAFDMEFRFRRKDGSWMWIRGRGRRVESDANGNAVRMVGTHIDITELKQQQQEIQYL
                     SFHDHLTGLYNRRFFDEELARLDTPRNMPLTLFLMDVNGLKLTNDAFGHQAGDRLLRT
                     VADVLRRTCREDEIIARIGGDEFILLLPQTGEEDAASLGIRLREAFENVRMETGTLSV
                     AFGWATKNDMSQPAGNVFITAENRMYQQKIYEMNSVRSKAISFILETLYENIPRERRH
                     SENVGVLSGRVGAAM"
BASE COUNT          250 a          451 c          414 g          309 t
ORIGIN      
        1 ttcattgctg cgccgacccg cccggagagg acgccgacgt tctccgagtg ccggcgctcc
       61 cggggaatat tctcgtacag cgtctccagg atgaaggaga tggctttgct gcggacgctg
      121 ttcatctcgt aaattttctg ctggtacatc ctgttttcag cggtgatgaa gacgtttccc
      181 gcaggttgcg acatgtcgtt cttggttgcc cagccgaatg cgacggaaag agtccccgtc
      241 tccatcctga cattctcaaa ggcctcccgc aatcgtatgc cgagcgacgc ggcatcctct
      301 tcaccggttt ggggaagcag aaggatgaac tcgtcgcctc ctatgcgggc gataatctcg
      361 tcctccctgc aggttcggcg gagcacgtcc gccacggtgc gcagcaggcg gtcccccgcc
      421 tgatggccga acgcgtcgtt cgtcagcttg agcccgttga cgtccatgag aaagagcgtc
      481 agcggcatgt ttctcggagt gtccaggcgc gcaagctctt cgtcgaagaa acgccggttg
      541 tacagtccgg tgaggtggtc gtgaaagctg aggtattgaa tctcctgctg ctgttgtttc
      601 agctcggtaa tgtcgatgtg cgtcccgacc atccggacgg cattgccgtt cgcgtcggac
      661 tcgacgcgcc gtccccgccc gcggatccac atccacgagc cgtccttccg gcgaaagcgg
      721 aactccatgt cgaacgcggg cgtctttccg gagaaatacg actgaagagc gcgattacat
      781 aaaggatggt cgtccggatg cagccgcttc gcccactcgt cgaacgagga gggaaactcg
      841 cccggctcgt agccggccat cgtgtagtac cgagggtcga agtacacctt gtccgtgacc
      901 aggttccagt cccacaggcc gtcgttggtc gccgccattg ccagctcgta gcgctcacgg
      961 cttatgcgca gcttctcctc cgctctcttc cggtcggtga tgtcggtgat gacggcgcag
     1021 agccccgtga tcgttccgtc ctcgccgtgt accgggaact tgcccgagag gaagaaatgc
     1081 cggccgtcgg ggcggtcgaa aacctcctcg aactcgacaa gctctcctct gcggagcact
     1141 tcggagtcgt tgcggcggaa gtgttcggct gcttccgggg agaagagctc ggcgtccgtc
     1201 cttcccagca cgtcggccct gggcagaccg gtgcactcct cccatctgcg gttcacgaat
     1261 gtatacactc cgtcgacgct cttgatgacc atcaacgagc cgctgttctc tatcagatca
     1321 gcgaggaaac gttccgtttc gagcaacttg ccttcagcat cgagaaggcg ttcccgcaac
     1381 tctcgcagtt cctccattcg ctctccccca tccggacacg cttt
//
LOCUS       DAOSWW010000810         1421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_82788_length_1421_cov_2.600293, whole genome shotgun sequence.
ACCESSION   DAOSWW010000810 DAOSWW010000000
VERSION     DAOSWW010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82788_length_1421_cov_2.600293"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..555)
                     /locus_tag="PK442_13260"
     CDS             complement(304..555)
                     /locus_tag="PK442_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88665.1"
                     /translation="MERDIEYNDVSWENPVVTEYRRIYGFQLEPATLDYLRKVAAQLR
                     YLQMVLHEYDLAVSKDTCEKTFQQRVGIAAETKSGSGRD"
     gene            complement(659..>1421)
                     /locus_tag="PK442_13265"
     CDS             complement(659..>1421)
                     /locus_tag="PK442_13265"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="McrC protein together with McrB forms the McrBC
                     restriction system; recognizes N4- and C5-methylcytosine
                     (and 5-hydroxy-methylcytosines); appears to act against
                     5-methylcytosine preceded by a purine residue; MrcC
                     modulates the specificty of McrB and has DNA cleavage
                     activity; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HOO88666.1"
                     /translation="DVFSEDTPMNRILKTAMPLLMRHGDVKKENSGLLRKLLLYFSDV
                     SPLSPYGIDWNALTYHRHNATYKMLLNICRLVFDGLLLSSKDGGCTLAEFIDDQQMHR
                     LFEKFVLEYYRKEYPQFKASASHIDWNIDGADSALFLPLMKSDITLTHAGRSMIIDTK
                     YYSRSMQSNPQFQSTSLISGNLYQIFTYVKNKDREVMGNVSGVLLYAKTDETVTPDCD
                     YRICGNRIAVKTLNLGAEWLEIKRQLDDLTLYITE"
BASE COUNT          281 a          391 c          384 g          365 t
ORIGIN      
        1 tcccggagct gttggcgccg aagaataaag atatcggagc catgcggatg agtccggtat
       61 ctttccaccc cttgaagttt tgtattcgca actgccgaag catccgcatc tcctcctggt
      121 cctgttatcg tctttggaaa gatgaaccgg aaactctccg gtactttccc gccgactacc
      181 ccggtacttc cttggcgact tccacggtcg ggtgaaaatc accccgctcg gtagcggcat
      241 tgcaatcgca agagcggccc gcatagtgtg agagcgacga cttggtcccg acttggtccc
      301 gacttagtcc cgacctgatc ccgacttcgt ctccgcagcg atgccgacgc gctgctggaa
      361 tgtcttctcg catgtatcct tggagaccgc caagtcgtat tcgtgcaaga ccatctggag
      421 atagcgcagt tgggcggcga ctttccgaag atagtccaag gtcgcgggct ccaactgaaa
      481 gccgtatatc ctgcggtatt ccgtcaccac cggattctcc catgaaacgt cgttatactc
      541 gatatcccgt tccaactgct ccgcgatatc gatgaagcgg tactcctgat ggtcgaaatg
      601 tcctccgctt attgattcat ccttcttcca gcttttttat atagtcgtgc gtacaggctc
      661 actctgtgat atacagcgtc aaatcgtcga gctgtctctt gatttcaagc cactccgcac
      721 cgaggttcaa tgtcttcacg gctattctgt tgccgcaaat ccggtagtcg cagtccggtg
      781 tgactgtttc gtccgttttg gcgtagagca gtacgccgct gacgttgccc atcacctcgc
      841 gatccttgtt ttttacgtag gtgaagatct ggtacaggtt gcccgagatc agcgacgtgc
      901 tttgaaactg aggattcgac tgcatactgc ggctgtagta ctttgtgtcg atgatcatcg
      961 atctgcccgc atgcgtcagg gtgatatcgg atttcatcag cggaaggaag agtgcgctgt
     1021 ccgcgccgtc gatgttccag tcgatgtggg aagcggaggc cttgaactgc ggatactcct
     1081 tcctgtagta ctcgaggacg aacttctcga aaagacggtg catctgctga tcgtcgatga
     1141 attcggccag tgtgcagcct ccgtctttgc ttgagagcag caggccgtcg aacaccagac
     1201 ggcagatgtt cagcagcatc ttgtatgtcg cgttgtgcct gtggtatgtc agcgcgttcc
     1261 agtcgatgcc gtacggggag agaggggaaa cgtccgagaa gtagagaagc agctttctca
     1321 aaaggccgct gttctctttt ttcacgtcgc cgtgccgcat gaggagcggc atcgccgtct
     1381 tcagtatccg gttcatcggc gtatcctcgg agaagacgtc g
//
LOCUS       DAOSWW010000811         1420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_82848_length_1420_cov_3.973626, whole genome shotgun sequence.
ACCESSION   DAOSWW010000811 DAOSWW010000000
VERSION     DAOSWW010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82848_length_1420_cov_3.973626"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="PK442_13270"
     CDS             complement(<1..1398)
                     /locus_tag="PK442_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOO88667.1"
                     /translation="MSTSLIRELVNVKLFQRGLDAKLQDHSRIGIPVHDLETMMLHPN
                     RENSNTTLNPESINLSVAELALKQYALTRVFSGDVAEAHLSGNIHLHDLGMVNRPYCS
                     GQSIAYVAKYGLNLPSITSISAPARHPDVLLAHLLKMTSVLQNNFAGAIGWDAVNTFF
                     APYLEGLSDERIRQIAQMLIFEFNQLAGGRGGQVAFTDVNLYYEVPKHFRDVPAIGPG
                     GKATGKTYGDYASEAKRFLRALFSVYMEGDSRGQPFFFPKPLLHITEDFFREDGWEEM
                     LSLACEVASEKGNTYFVFDRGDAVKLSECCRLSFELDHRDILEARTPWKLRFCALQNV
                     TLNLPRMAYRSGGDRETLFELVDHYMEIAAKAHLQKKKFISEILDLGKRGPLSVLCME
                     HDGEPYLRFERVKYLVGILGMNEMVQTITGRQLHESEEAFQLALAVTQYMNLKCQALS
                     ERYGLDIVLEQTPAES"
BASE COUNT          321 a          416 c          372 g          311 t
ORIGIN      
        1 gctctcggcg ggagtctgct caagcacgat atcgagtcca tagcgttccg aaagtgcctg
       61 gcatttcaaa ttcatgtact gcgtaaccgc cagcgcgagc tggaacgcct cttccgattc
      121 gtgaagctga cggcccgtga tcgtctggac catctcgttc atacctaata tacctacaag
      181 gtacttcacc ctctcgaacc gaagatacgg ttcgccgtcg tgttccatac agagaacaga
      241 aagcgggcct cgcttcccaa gatcaagtat ctcactgatg aattttttct tctgcaggtg
      301 cgctttcgcg gcaatctcca tgtaatggtc taccagctcg aagagcgtct ccctgtctcc
      361 gccgctcctg tacgccattc tggggaggtt gagcgtcacg ttctgcaggg cgcagaagcg
      421 aagcttccac ggtgttctcg cttccaaaat atccctgtga tcaagctcga agcttaaacg
      481 gcagcactcg gagagcttga ccgcatctcc ccggtcgaag acaaaatagg tgtttccctt
      541 ttcggacgcg acctcgcagg caagggagag catctcctcc catccgtcct cgcggaaaaa
      601 gtcctctgtg atgtgcagca gtggctttgg gaagaaaaag ggctgccccc ggctgtcgcc
      661 ctccatgtac accgaaaaga gcgctctcag gaaccgcttc gcttcggaag catagtcgcc
      721 gtatgtcttc cctgtcgcct tgcctcccgg accgatcgcc ggaacatccc gaaagtgctt
      781 cggcacctcg tagtacaggt tcacatccgt gaaggccacc tgaccgccgc gccctcctgc
      841 gagctgattg aactcgaaga tgagcatctg ggcaatctga cggatacgct catccgaaag
      901 accttcaaga tacggagcaa aaaaagtgtt cacagcatcc caaccgatcg cccccgcaaa
      961 attgttctgc agtacggagg tcatcttgag aagatgtgcg agcagcacgt ccgggtgacg
     1021 cgcaggagcg gatatgctcg tgatggaggg aagattcaga ccatacttcg ccacataggc
     1081 gatgctctgc ccggaacagt acggacggtt caccataccg agatcatgaa ggtggatatt
     1141 ccccgaaaga tgcgcctcgg cgacatctcc ggaaaagacg cgcgtaaggg cgtactgctt
     1201 cagagccagt tcagccaccg acaggttgat tgactcggga ttgagagtgg tattgctgtt
     1261 ctccctgttc ggatgaagca tcatggtttc aagatcgtgc accggaattc ctatccggct
     1321 gtggtcttgc agctttgcgt ccaatccgcg ctgaaaaagc ttgacgttca ccagttcgcg
     1381 aataagcgac gtgctcaccc ggtcgcgtcc ccaacgcaag
//
LOCUS       DAOSWW010000812         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_83013_length_1419_cov_1.873900, whole genome shotgun sequence.
ACCESSION   DAOSWW010000812 DAOSWW010000000
VERSION     DAOSWW010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83013_length_1419_cov_1.873900"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PK442_13275"
     CDS             <1..1119
                     /locus_tag="PK442_13275"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88668.1"
                     /translation="PAVTIIFIAGFLYARRPFTVPVTLFYDERDLAGAVVSKQALQSV
                     VLAMEFFNSRSSEFRFLPFRETDQDIYESIEHAAARGSIAIVGGINAPFASLLADSSR
                     RHGIPFLSLASGTSLARADDFVFRARPRSGGKELGLAAKHLGVSSYSVIVSGFAASHV
                     QEFIRDFESQLGAPPRRTMVFSGNLNKHIEDFERIAKGVEAILLVLPDWLAAVALREL
                     RLRLPGLPVFASNWAVSHRTHLLAGSLGEGVVTASFTHPGWDDPEHDFLRFVRDTYAS
                     PIPPVILAMGYDTVAMLDEAIRRAGSSDKRAIAEALAELTSVRTTGGTIPVDKNGDMQ
                     HTGALFSLRSGRWIRVPGNDPASLRRATTSFRPQRGEL"
     gene            1116..>1419
                     /locus_tag="PK442_13280"
     CDS             1116..>1419
                     /locus_tag="PK442_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88669.1"
                     /translation="MSKKFTFSIGTFFSVVVISFLLIFIVQGATTLYMRVSARLDEFA
                     RHGENVLQVTSLLLEKQVEQREEGLRAIVGGADMGDAGRDVLVAAVLDGNTVKRGIK"
BASE COUNT          234 a          473 c          428 g          284 t
ORIGIN      
        1 ccggcggtca caatcatttt catcgcaggt ttcctgtatg cacgcaggcc gttcacggtc
       61 ccggtgaccc tgttctacga cgaacgcgac ctcgcgggcg cagtggtgtc gaaacaggct
      121 cttcagtcgg tcgtgctggc catggaattc ttcaactccc gttcctcgga atttcgcttc
      181 cttcctttca gagaaaccga tcaggatatc tacgaatcca tcgaacatgc cgccgcacgc
      241 ggctccatag cgatcgtcgg aggcatcaac gcgccgttcg cctcccttct ggcggattcc
      301 tcaaggcgtc acggcattcc cttcctctcc ctggcctcgg gcacgtcgct cgcgcgcgcg
      361 gacgacttcg tcttcagggc gcgtccccgg agcgggggca aggaactcgg gctcgcggcg
      421 aagcatctgg gcgtctcctc ctattcggtg atcgtcagcg gattcgccgc ctcgcacgtg
      481 caggagttca tccgcgactt cgagtcgcag ctcggcgctc ccccgcgcag gacgatggtc
      541 ttcagcggca acctgaacaa acacatcgag gacttcgagc gcatcgccaa gggggtggag
      601 gccatactcc tcgttcttcc cgactggctt gcggccgttg cgctgcggga gcttcggctc
      661 cggctgcccg gcctccccgt cttcgcctcc aactgggccg tctcgcacag gacgcatctg
      721 ctcgccggct cgctcggcga gggcgtcgtc acagcttcgt tcactcaccc cgggtgggac
      781 gacccggaac acgatttcct ccgcttcgtg cgcgacacgt acgcgtcccc cattcctccg
      841 gtcatcctcg cgatggggta cgacacggtc gccatgctcg acgaggcgat ccggcgcgcg
      901 ggctcgtccg acaagagggc catagcggag gccctcgccg agttgacgag cgtccgcacc
      961 acaggaggta ctatccctgt cgataaaaac ggggacatgc agcataccgg agcgttgttc
     1021 agtctccgct ccgggcggtg gatccgtgtt cccggaaacg atccggcgtc gctccggcgg
     1081 gcaaccacgt cttttcgccc gcagcggggg gagctatgag caaaaaattc accttctcca
     1141 tcgggacgtt cttctcggtc gtcgtcatta gtttccttct tattttcatc gtccaggggg
     1201 cgaccaccct ctacatgaga gtgagcgcaa ggctcgacga atttgcgcgt cacggcgaaa
     1261 acgtgctcca ggtgacctcg cttctgcttg aaaagcaggt ggagcagcgc gaggagggac
     1321 tgcgcgccat cgtcggcggc gcggatatgg gcgacgcggg gcgcgacgtc ctcgttgccg
     1381 ccgttctcga cggaaatact gtgaagcgcg gcatcaagg
//
LOCUS       DAOSWW010000813         1414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_83445_length_1414_cov_2.890361, whole genome shotgun sequence.
ACCESSION   DAOSWW010000813 DAOSWW010000000
VERSION     DAOSWW010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83445_length_1414_cov_2.890361"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK442_13285"
     CDS             complement(<1..257)
                     /locus_tag="PK442_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HOO88670.1"
                     /translation="MKRTLSFFSRGRIRPLLFGEPLNGKDRSVLLRQMKGRVFDPSLV
                     SAVVRRCPKGFPVVIQCVPLKKGKPFPTSFWLTCPWLVRLCG"
     gene            complement(254..697)
                     /locus_tag="PK442_13290"
     CDS             complement(254..697)
                     /locus_tag="PK442_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOO88671.1"
                     /translation="MADEKKGDVTPLAPGEVVVCVVEQIMPYGAFVRIKNGQRAMIHI
                     SELSHGFVKKVEDILALGQEVQARVIKIDEKGRIDLSLKKMEAPRPAASFAPRSAAAN
                     VPEDPRDSFEKKLSNYLKASEAKIADINSKLNSSKASKKRGKPAK"
     gene            complement(813..>1414)
                     /locus_tag="PK442_13295"
     CDS             complement(813..>1414)
                     /locus_tag="PK442_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88672.1"
                     /translation="RLFLGKTKVRSLRPQGKEVVLRTTGRVIETHPLRTPERVLGSAL
                     FGSAMDVLTKGFCISEAAIGGIRGNIRGGMSAFDRRLVVNGERVVRDTRVFLHSIGGF
                     TVRCNKAVGRISPLPIEVRNRLPWFRGRSNSLKKGEVLLALYCPILEDGVLKSVLNKE
                     RGTLLVWYNPRRAWKARSLFLVRDFSSGGSLEWKWIDIH"
BASE COUNT          333 a          413 c          351 g          317 t
ORIGIN      
        1 ccgcaaagcc tgacgagcca aggacaggtc agccagaacg atgtggggaa cggcttgcct
       61 tttttgagag gaacgcactg tatcacaaca gggaagcctt tcggacagcg gcgcacaaca
      121 gcggaaacca gcgaagggtc gaaaacacgc cctttcatct ggcgcagcag aacagatcga
      181 tcctttccat tcagcggctc cccaaaaaga agggggcgaa tccgcccccg ggagaaaaaa
      241 gaaagagtcc gcttcacttc gcaggtttgc ctcttttctt tgaagccttg ctgctgttga
      301 gtttgctgtt gatatcggcg atcttcgcct cgctggcctt gagatagttc gaaagcttct
      361 tctcgaagga gtcccgggga tcttcaggaa cgttcgccgc agcgcttcgc ggagcaaagg
      421 aagcagccgg ccggggggcc tccatttttt tcagggagag atcgatcctg cccttctcgt
      481 cgatctttat taccctggcc tggacctcct ggccaagagc gaggatatcc tcgactttct
      541 tgacgaaccc gtgggacagc tcggagatat ggatcatcgc ccgctgtccg tttttaatcc
      601 gaacaaaagc gccgtagggc ataatctgct ctacaacgca cacaaccact tcacccggtg
      661 ccaggggagt gacgtctccc tttttttcat ctgccatgcg gttgtctacc ctccacgatt
      721 atttgttgat actttgagtc cttactgcat cgacaagctg tgccgctgtt tcgtttccgg
      781 tgaaaaccac ctccgcaggc tgcgattatg cgctagtgaa tatcgatcca tttccattcg
      841 agactccccc cggaggagaa gtcccgaaca agaaacaaac tccgggcttt ccacgccctt
      901 cgaggattat accaaacaag aagcgttcct ctctccttgt tcagaacact tttcaacacg
      961 ccgtcttcca gtatcgggca gtaaagcgca agcaaaactt ctcccttctt gagagaattc
     1021 gaccttcctc gaaaccaggg aagccgattc cgcacttcaa tcggaagggg cgagatcctg
     1081 cctacagcct tgttacaacg tacagtgaat ccaccgatgg agtgcaaaaa aacccgcgta
     1141 tcgcgcacaa ctctttcgcc gttcaccacg aggcgcctgt cgaaagcgga catcccgccg
     1201 cggatattgc cccgaatacc tccgatcgcc gcctctgaaa tacaaaagcc ctttgtaagc
     1261 acatccatcg cagaaccgaa cagggcggaa ccaagtacgc gttcgggagt gcgaagaggg
     1321 tgcgtttcta taactctccc ggtagtccga agcacgacct ctttcccttg agggcggagg
     1381 gaacgtactt tcgtttttcc gagaaagagc cttg
//
LOCUS       DAOSWW010000814         1412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_83636_length_1412_cov_3.186441, whole genome shotgun sequence.
ACCESSION   DAOSWW010000814 DAOSWW010000000
VERSION     DAOSWW010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83636_length_1412_cov_3.186441"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PK442_13300"
     CDS             complement(<1..710)
                     /locus_tag="PK442_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO88673.1"
                     /translation="MTFYPWQLDAFQAIRETSAVLSAPTGSGKTLVAYLWAGILDEAG
                     TVLPPGDGRIIFTAPIKALSNERYLDLRKLGLDVGIETGDFKKNEGAGIICCTQEIYS
                     LKYARVPGQKLIVDEFHYIFDDSDRARTYIDGIRNTDESTAVLVMSATLGGASSVVKY
                     LEGVTGRSFLLYENAKRETQLLFTPERPAYMDGVKDALVFLFSQKGAMELAYLIARQR
                     KRIHSSKRERLAELAAILG"
     gene            826..1290
                     /locus_tag="PK442_13305"
     CDS             826..1290
                     /locus_tag="PK442_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88674.1"
                     /translation="MAEFVIVVQEEDDEARQACVYGLEPRTPFLSKRREAAFWKTIGE
                     EVTEAVASSGLPFDAAMLFIKKARRASMKRVSLCALAHDAGEDGWRVLTSSSKPGEQA
                     SPMTEDEKLQFEEARLLAEEISKRNRDAIPETDDEIISVEEFEDMLRGESED"
BASE COUNT          301 a          354 c          430 g          327 t
ORIGIN      
        1 cccagaatcg ccgcaagctc cgcaaggcgt tctcgcttgg atgagtgaat gcgcttcctc
       61 tggcgggcga tcaggtaggc gagctccatc gccccctttt gcgaaaagag gaagactagc
      121 gcgtccttca cgccgtccat gtacgcaggg cgttccggcg tgaagaggag ttgtgtttcg
      181 cgcttcgcgt tttcatagag caggaaggag cgtccagtca ctccctcaag atattttacg
      241 accgaagacg cgcctccgag ggttgcggac atgaccagga cggcggttga ctcgtcggtg
      301 ttccggatgc cgtcgatgta ggttcgcgcc cggtccgaat cgtcgaagat gtagtggaac
      361 tcgtccacta tgagtttttg tccggggacg cgggcgtact tcaaggaata gatctcctgc
      421 gtgcagcaga ttatgcctgc gccttcgttc tttttgaagt cgccggtttc gattcccacg
      481 tcaagcccga gtttcctgag atcgagatag cgttcattgc tcagcgcctt tatcggggcg
      541 gtgaagatga tgcgtccgtc cccgggagga aggaccgttc cggcctcgtc aagtatgccc
      601 gcccaaagat aggccaccag cgtttttccg gatccggtag gggccgagag cacagcgctt
      661 gtctctcgaa tagcctgaaa ggcgtccagt tgccacggat aaaaagttat atgcgccagc
      721 gttgaaactc ctctcgaaag tgcgtagggt tgttcttcca gaaagatatg gtaccacatc
      781 tttcgtggta ttataaagtg aatcaatccg gcggaggtgt tgctcatggc agagtttgtg
      841 atagtggtac aagaagagga cgatgaggcg cgccaagcgt gcgtctacgg acttgaaccg
      901 cgtacgccct ttctttcaaa aagaagagaa gcagctttct ggaaaacgat aggggaagag
      961 gtcacagaag cagttgcatc ctccggcctt cccttcgatg cggcgatgct ctttataaaa
     1021 aaagccaggc gcgcctccat gaagagagtg tccctctgcg ctcttgcgca cgacgccgga
     1081 gaagacggat ggcgtgtgct cacatcatcc tctaagcccg gagagcaggc gtctccgatg
     1141 acggaggacg agaagcttca gttcgaagag gcgcgtcttc ttgccgaaga gatctcaaaa
     1201 cgtaaccggg atgcgattcc cgaaacagat gacgagatca tttcggtcga ggagtttgag
     1261 gatatgcttc ggggggagtc cgaggactaa gtcttttttg gcgccggacc ttcctttgtg
     1321 gcgggcccgg tttgctgtgt gttttaggag tgaatactaa ggggcggggg atgttttcgt
     1381 gacgtggagc agtgcgttcc aaattctcgg cg
//
LOCUS       DAOSWW010000815         1410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_83801_length_1410_cov_4.602214, whole genome shotgun sequence.
ACCESSION   DAOSWW010000815 DAOSWW010000000
VERSION     DAOSWW010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83801_length_1410_cov_4.602214"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PK442_13310"
     CDS             <1..447
                     /locus_tag="PK442_13310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO88675.1"
                     /translation="SWISLGLVSAMDENADSLSRTHGDAIIEATTDETLDSLPEEHVL
                     ALVRIMQEAVSNSVRHGRARSIQVRVRDEGCAVRFSIRDDGKGFDPGEHPGTEYERLR
                     TSGHRGLANISERVRLLRGTMRLESRPGEGCGIDIVFPDPRARAAD"
     gene            622..>1410
                     /locus_tag="PK442_13315"
     CDS             622..>1410
                     /locus_tag="PK442_13315"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HOO88676.1"
                     /translation="MEKILDIVFNLGMLASLSILSGFIGADGERRARKLLMQGALYGT
                     ASLLGMLHPLVLAPGVIFDGRSVMLSVCGFFFGPAAAALAASMALVLRVHQGGSGAVM
                     GVSVIVSSALIGALFHRMHYSRSRRASARLLYLMGLLVHAAMVLLMFTLPEDQSMKSI
                     RLLALPVMIAYPLTTLFIGRILSEFDAHRRMGESLKLSESNLRRSNEELAANLNKLMK
                     REKELRFANAVLLTQQETSLDGILVVDGEGGIISFNRRFTELWGI"
BASE COUNT          262 a          457 c          423 g          268 t
ORIGIN      
        1 tcctggatca gcctcggcct cgtcagcgcg atggacgaga acgccgattc gctctcccgc
       61 acgcacggcg acgcgatcat cgaagcgacg accgacgaga cgctggattc gctccccgag
      121 gagcatgtat tggcgctcgt ccgcataatg caggaagccg tgtcgaactc cgtccggcac
      181 ggaagagcgc gctcgattca ggtacgcgta cgggacgaag gatgcgctgt ccgcttctcg
      241 atccgggacg acggtaaggg gttcgacccc ggcgaacacc ccggaacgga gtacgaacgc
      301 ctccggacct ccggccacag agggctggcc aacatcagcg agcgtgtccg tctgctccgc
      361 gggacgatgc gcctggaatc gcgccccggc gaggggtgcg gtatcgacat cgtctttccc
      421 gatccgcgcg cgcgcgccgc ggactgacag ggaggaggct accgccttct ctccgcgctc
      481 aaacagaacg cattgttcta cattaatttt tgacgctcgt actttataat aatgaaagca
      541 gagaagaatc cgaacggcgg gcgccttcga agaacgcccc cgcatacaag gatattttct
      601 gtaaaggagc tgaaacggcc tatggagaag atcctggaca tcgtcttcaa cctcggaatg
      661 ctcgcttcgc tgagcatttt gtcgggcttc ataggcgccg acggggaacg ccgggcgcga
      721 aaactcctga tgcagggagc cctctacgga acggcctctc tcttggggat gctccacccc
      781 cttgtgctcg cgccgggggt catcttcgac ggccggtcgg taatgctgag cgtctgcggc
      841 ttcttcttcg gcccggccgc cgctgctctc gcggcgtcga tggcgcttgt cctccgtgtc
      901 catcaaggag gctcgggggc cgtgatggga gtctcggtca tcgtctcctc cgccctcatc
      961 ggcgcgctct tccaccgcat gcactacagc agatcgcgca gggcctccgc ccgtcttctc
     1021 tacctcatgg ggctgctggt gcacgcggcg atggtgctgc tgatgttcac acttcccgaa
     1081 gaccagtcga tgaagagcat ccgcctcctc gccctccccg tgatgatcgc gtacccgctg
     1141 acgacgctct tcatcggcag gattctctcg gagttcgacg cgcatcgccg gatgggcgag
     1201 tcgctcaagc tcagcgagtc gaatctccgc agatccaacg aagagctggc cgccaatctg
     1261 aacaagctca tgaaacggga gaaagagctg cgcttcgcga acgccgtact cctcacacag
     1321 caggagacgt cgctcgacgg catcctcgtc gtcgacggcg aaggaggcat catctccttc
     1381 aaccgtcgct tcacggagct gtggggcatt
//
LOCUS       DAOSWW010000816         1407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_84145_length_1407_cov_2.048817, whole genome shotgun sequence.
ACCESSION   DAOSWW010000816 DAOSWW010000000
VERSION     DAOSWW010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84145_length_1407_cov_2.048817"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PK442_13320"
     CDS             <1..785
                     /locus_tag="PK442_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO88677.1"
                     /translation="GNAVKFTETGGVRFCATADGNAVSINIEDTGIGIEADELPFIFD
                     EFRQSDGSMARKFEGTGLGLAIAKKYAEILGIDISVVSKPGSGTAFTLRIPLSPVMPG
                     DAHPSGAEPRETSADTPARRRKPHLHVVEDNPVATMHIRRMLEPEGFRVSTFPGGRES
                     LSSIETDIPDGIVLDLMMPGMDGFEFLKELRRRKHDIPVLVLTAKVLTREDTKFLNEN
                     GIRFLALKGDVDPGDLVRKIRTMTSQGAPLADKESLGEGTEK"
     gene            782..>1407
                     /locus_tag="PK442_13325"
     CDS             782..>1407
                     /locus_tag="PK442_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88678.1"
                     /translation="MNRILAIDDKADNLVVLRAMMKMLLPECVLLQALSGEEGLRIAK
                     EQRPDTILLDIFMPGMDGYETTMRLKADPETASIPVILLTAAENTAANRTAGLKVGAD
                     AFLSKPIEEEELVAQVRAMLRVKESETALKTKMDVLEDLVEQRTKELKESANRLALTL
                     DQTISVLANTVETKDPYTAGHQVRCASLALALGNELRLDPQQLRGIHMA"
BASE COUNT          318 a          442 c          414 g          233 t
ORIGIN      
        1 caggcaacgc ggtaaagttc accgagacgg gcggcgtgcg cttttgtgca actgccgacg
       61 gcaacgcagt ctccatcaac atcgaagaca ccggcatagg catagaagcg gacgaactgc
      121 cgtttatctt cgacgaattc cgccagagcg acggctcgat ggcgcgcaaa ttcgagggaa
      181 ccgggctcgg actcgccatt gcgaaaaagt acgccgagat cctcggcatc gacatctccg
      241 tggtcagcaa accgggaagc ggaaccgcat ttacgctgcg cattccgttg tcgcctgtca
      301 tgcccggaga cgcacatcct tccggcgccg aacctcgtga aacctctgcg gacaccccag
      361 cgcgccgccg caaacctcat cttcatgtcg tcgaggacaa cccggttgcg acgatgcaca
      421 tccgccggat gctcgaaccg gagggcttcc gggtttccac ctttcccggc ggaagagagt
      481 cgctcagctc gatagagacg gacattcccg acggcatcgt cctcgacctc atgatgcccg
      541 gtatggacgg cttcgaattt ctgaaagaac tgcgcaggcg gaagcacgac attcctgtcc
      601 ttgtcctcac cgccaaggta ctcacacggg aggacacaaa atttctcaat gagaacggaa
      661 ttcgctttct tgccctcaag ggggacgtcg accccggaga cctggtgcgg aaaatccgga
      721 cgatgacctc gcagggtgcg ccccttgcgg acaaagagag cctcggggag ggaaccgaaa
      781 agtgaacagg atacttgcga tcgacgataa ggccgataat ctcgtggtgc ttcgggcaat
      841 gatgaagatg ctccttcccg aatgcgttct gctgcaggcg ctctcgggtg aagagggact
      901 ccgcatcgcc aaggagcagc gccccgatac gattctgctc gacattttca tgccggggat
      961 ggacggctat gaaacgacga tgcgcctcaa ggccgaccct gaaacggcat ccattccggt
     1021 gatcctcctg accgccgccg aaaacacggc ggcgaaccgc accgcgggac tgaaggtcgg
     1081 cgccgacgcg ttcctctcca agcccatcga ggaggaggaa ctggttgcgc aggtgcgcgc
     1141 gatgctccgc gtcaaggagt ccgaaacggc gctgaagacg aagatggacg tactcgaaga
     1201 tctggtcgaa cagagaacca aagagctgaa ggagagcgcg aaccgccttg ccctgaccct
     1261 cgaccagacc atctccgtgc tggcgaacac cgtggagaca aaagacccgt acacggcggg
     1321 acatcaggtc cgctgcgcct ccctcgccct cgccctgggg aacgagctgc ggctcgaccc
     1381 ccagcagctt cgcggcatcc acatggc
//
LOCUS       DAOSWW010000817         1403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_84502_length_1403_cov_3.565282, whole genome shotgun sequence.
ACCESSION   DAOSWW010000817 DAOSWW010000000
VERSION     DAOSWW010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84502_length_1403_cov_3.565282"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PK442_13330"
     CDS             complement(<1..1181)
                     /locus_tag="PK442_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88679.1"
                     /translation="MLYRFSHSLPPFAANGKRSMYRVVPALVFFLLSLALVFPALKAN
                     AAASVRKDAFIAQLFQARGFSLPANEKSPVNAALEYNLTPLPEGKLSDPLTRREAIVY
                     AVHSLGLFFESRILADYPLPYKDVSGLLPEERGALAVALNMKPPLLKKGSATFSPSHA
                     VSPAEANEIAARVKAATRELRLSVDLSPLKGMTIRIRREGVLSILPKWRAVVNGFESR
                     EDADFFRGLLTERGVEATVDSYNYDWRVRSPIFDMYGGVRKFLSAASALGREGVVFAS
                     IPNWEMTDTPRFWTMMVFDPGAFELRPVFPSEGMAALAPLSSMLRDGAVAVVNGGYFG
                     TSGKGRGSPIGALMTDGVLLNPPFAGRTCFGWNSENRAVFGQLTWNGKVHLPGGFMEL
                     SG"
BASE COUNT          328 a          418 c          427 g          230 t
ORIGIN      
        1 ccgctgagct ccatgaaccc tcccggaaga tgcaccttcc cgttccacgt aagctgaccg
       61 aagaccgccc tgttctcgct gttccatccg aagcaggtac gcccggcgaa cggaggattc
      121 agcagcaccc cgtcggtcat cagcgcgccg atagggcttc cgcggccttt ccccgacgtc
      181 ccgaaatacc ctccgttcac aacggcgacg gcgccgtcgc ggagcatgga gctcaaggga
      241 gcgagcgccg ccattccctc ggagggaaag acagggcgca gttcaaacgc cccgggatcg
      301 aagaccatca tcgtccagaa gcgcggcgta tcggtcatct cccagttcgg aatgctcgca
      361 aaaacaacgc cctctctgcc gagcgcggat gctgcggaaa gaaacttccg taccccgccg
      421 tacatgtcga agatgggaga gcgcacgcgc cagtcgtagt tgtagctgtc aacggtagcc
      481 tccacgccgc gttccgtaag caacccgcgg aaaaagtccg cgtcctctcg ggactcgaac
      541 ccgttcacaa ccgccctcca cttcggaagg attgagagca cgccttcccg gcgtatccgg
      601 atcgtcattc ccttgagcgg tgagaggtcc accgagaggc ggagctcgcg ggtcgccgcc
      661 ttcacacggg cggcgatctc attggcttcg gcgggagaga ctgcgtgcga cggagaaaaa
      721 gtcgccgaac cttttttcag caagggaggc ttcatgttca gggcaaccgc cagagcgccc
      781 cgctcctccg gcaaaagacc ggagacgtcc ttgtagggaa gcggatagtc ggcgaggata
      841 cgcgactcga agaagagccc gaggctgtgg acggcataca cgatcgcctc cctgcgcgtg
      901 agagggtcgg aaagctttcc ctccgggaga ggcgtcaggt tatattcaag agcagcgttg
      961 acaggagact tctcgtttgc aggcagcgaa aacccccgcg cctgaaagag ctgggcgata
     1021 aacgcatcct tgcggacgga agcggcggca ttcgctttca gggccgggaa gacaagggca
     1081 agagagagga ggaaaaagac gagggccggg actactctgt acatactcct cttcccgtta
     1141 gcggcgaagg ggggaagaga atgagaaaac cgatagagca tagtgtattc tccttgaggc
     1201 gctgtattcg aaacggcgaa gataattccg gatgccgcaa ctgcgcgcat ataacatgac
     1261 gagtgtacca gatgtgctcg aagcgcggca tacggaaaaa ggcgtaaaaa aaccgggacc
     1321 cgataatcgg accccggttc tggagaacaa aatggcgcgc cggacaagat tcgaactcgt
     1381 gacctgcggt tccgtagacc gcc
//
LOCUS       DAOSWW010000818         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_84594_length_1402_cov_4.187082, whole genome shotgun sequence.
ACCESSION   DAOSWW010000818 DAOSWW010000000
VERSION     DAOSWW010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84594_length_1402_cov_4.187082"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PK442_13335"
     CDS             <1..740
                     /locus_tag="PK442_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88680.1"
                     /translation="GRPPAEKGNGEVVSVFDPCASRNRPESQRAARSLVRGAGYELAP
                     LPYEGKRAQCCSYGGQIDIAAPKYAQWLAEQRSKDGEAPYVVYCSNCRDVFAHAGKPA
                     RHILDIFFGLNGWNAAPPTFDERRKRREAMKNDLVQEFWPDMELRGKEEIMDNRKSIA
                     ITPELREKMNRERLLEEDALAVIEECERSGRRVWDPSAERFFGCKEIGSMTQWVEYAP
                     SPEGYALFNTYAHRMKIEREDAPHGRK"
     gene            727..921
                     /locus_tag="PK442_13340"
     CDS             727..921
                     /locus_tag="PK442_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88681.1"
                     /translation="MEENNKLFCGKCMVEMLPAPTGFSYLGHAFRTDVLRCPSCGQVF
                     IPESLAKGRMAEVETTLEDK"
     gene            complement(983..>1402)
                     /locus_tag="PK442_13345"
     CDS             complement(983..>1402)
                     /locus_tag="PK442_13345"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOO88682.1"
                     /translation="GGKTVHCKDASPKLCGEKMGARLSDELGARGRVAIVGYQPAILK
                     GLTGALGPERVCVTDLNPENIGRCVCGVTVLDGEKDLQRAAAWCDAALVTGSTLSNGT
                     IDALLDAFSGKRIVFFGTTIAVPAELLGFERLCFEAE"
BASE COUNT          293 a          453 c          444 g          212 t
ORIGIN      
        1 ccgggcgtcc tcccgcggaa aaggggaacg gggaggtcgt ctccgtcttc gacccgtgcg
       61 cgagccgcaa cagaccggaa tcgcagcgcg cggcgcgctc gctcgtccgc ggcgcagggt
      121 acgaactcgc tcccctcccc tacgagggaa agcgggcgca gtgctgctcc tacggcgggc
      181 agatcgacat cgccgcgccg aagtacgcgc aatggctggc ggagcagaga agcaaggacg
      241 gcgaggctcc gtacgtcgtc tactgctcca actgccgcga cgtcttcgcg cacgcgggga
      301 agcccgcgcg ccatattctc gacatcttct tcgggctgaa cggatggaac gccgcgccgc
      361 cgacgttcga cgagcgcaga aaacggcgcg aagccatgaa aaatgatctt gttcaggagt
      421 tctggcccga tatggaactc cggggaaaag aggagatcat ggacaatcga aaaagtatcg
      481 ccataactcc cgaactgcgg gagaagatga accgcgagcg gcttctggag gaggacgcgc
      541 tcgccgtgat cgaggagtgc gagagaagcg gccgcagagt atgggatccg tccgcggaac
      601 gcttcttcgg ctgcaaggag atcggctcca tgacgcagtg ggtggagtac gcgccgtccc
      661 ccgaaggata cgcgctcttc aacacctatg cgcacagaat gaagatcgag cgggaggacg
      721 cccctcatgg aagaaaataa taaactcttc tgcgggaagt gcatggtgga gatgctcccc
      781 gccccgaccg ggttcagcta ccttggacac gccttccgca cggacgtgct gcgctgcccg
      841 tcgtgcgggc aagtcttcat cccggaaagt ctcgccaagg ggcgcatggc cgaagtggag
      901 acgacgctcg aagacaaata aaacggaggt gaagagacgg cacaaccaca gcggcgccgc
      961 ctcttcacct ccggaacacg ctttactccg cctcgaaaca gagcctctca aagcccagaa
     1021 gctcggcagg gactgcgatc gtcgttccga aaaagacgat acgcttcccg gagaacgcgt
     1081 caaggagcgc gtcgatcgtc ccgttcgaga gcgtggagcc cgtcacgagg gcggcgtcgc
     1141 accacgcggc cgcgcgctgc aagtccttct cgccgtcgag aaccgtcacg ccgcatacgc
     1201 agcgtccgat gttctcgggg ttcagatccg tcacgcacac gcgctccggg ccgagcgccc
     1261 ccgtcagccc cttcagaatc gcaggctggt agccgacgat ggcgacgcgg ccgcgcgcgc
     1321 caagttcgtc cgaaagcctc gcgcccatct tctcgccgca cagcttcggc gaggcgtcct
     1381 tacagtgcac ggtctttccg cc
//
LOCUS       DAOSWW010000819         1401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_84705_length_1401_cov_3.653046, whole genome shotgun sequence.
ACCESSION   DAOSWW010000819 DAOSWW010000000
VERSION     DAOSWW010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84705_length_1401_cov_3.653046"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PK442_13350"
     CDS             complement(<1..394)
                     /locus_tag="PK442_13350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO88683.1"
                     /translation="MNNGISKEVISQFDKISELPDCWDHNQQYQKHLLSKINTTQGVA
                     LDIGCGTGEFTKKLSSLFSKVIGIDVSPKMIDEAKKRNNPSNVDFINVDVETYLKTTE
                     EKYDLIISIATFHHLEYASVLNHIYDKLK"
     gene            996..1214
                     /locus_tag="PK442_13355"
     CDS             996..1214
                     /locus_tag="PK442_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOO88684.1"
                     /translation="MKAEFTAIIEAAPEGGYWAMCPEIPGANGQGETLEEAKESLREA
                     IYLILEDRREDVLRGLPDDVIRETVSVG"
BASE COUNT          362 a          329 c          341 g          369 t
ORIGIN      
        1 tcttcaactt atcgtaaatg tggtttaata cagatgcata ctctaaatga tgaaaagttg
       61 ctatagatat tatcaaatca tatttctctt cagtggtttt caagtatgtt tcaacatcta
      121 catttataaa atccacattt gaaggattat ttcttttttt cgcttcatcg atcatcttcg
      181 gggatacgtc aataccaata acttttgaga acaatgatga gagttttttt gtaaattctc
      241 ccgtaccgca accgatatcc aatgctaccc cttgtgttgt atttattttt gacaacaaat
      301 gcttttgata ctgctgatta tggtcccagc aatctggcag ttccgatatt ttatcaaatt
      361 gagaaatgac ttcttttgaa attccattat tcatagtgtt cccctcttat aaaacgagca
      421 tactactcaa caagcacgta gtaatatcaa ctgtacacag aatagtttca tagtatttac
      481 gccgaaccgg tccgggcgtt cttccaactg cgccgcttcg ataaccgtcg gttgcttggc
      541 ttccagcaat ctctctttca aacacgccca ctcgtacgtc gagtaaccta ctataagcag
      601 gatggctatg tcaagatccc gatctcgttc cgtgacgggg ctgtttttcg acaagaagct
      661 tgatgaggcc gagtaatccc cttctcgcac ctgcgacagt aaagggagga tgaggcggat
      721 cgtctcagga caaaaagagc ggagtcacga tgttggaacg ttgaaatcgg ttcattcagc
      781 gctctccaca ggtacgacct ccgcggcgtt gccttcgcgg aagagcatcc ctctcctgtc
      841 gaatcagacg acgcttctcc ggagaatgcc actgcgcttc tccgagggct ccttcgtgaa
      901 agtcggtatg tacatcattc ctgtatcttc aaggtgctat aattcgatgc gaaaccgcgt
      961 gcggcgctcc gcgcatcgca ggaggaggga agagtatgaa agccgaattc accgcgatta
     1021 tcgaagcggc gcccgaggga ggctactggg cgatgtgccc ggagattccc ggcgccaacg
     1081 gacagggaga gacgctcgaa gaggcgaaag agagcctgcg ggaagccata tatctgattt
     1141 tggaggaccg cagagaggac gttctacgcg gccttccgga cgatgtcata cgcgaaacag
     1201 tatcggtcgg atgaagcgaa aggatttgga acgcttgctt cgcatggcgg ggtgttattt
     1261 gaaacgcgag ggcgcttcgc atgctctgtg gatcaacccg aaaaccggta cgatggaagc
     1321 tgttcctcgg catgcggaga tcaaagaacc gttggcgagg aaaatactaa agagcctcga
     1381 cgcgtgagat ttaatggcga t
//
LOCUS       DAOSWW010000820         1398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_85033_length_1398_cov_2.675354, whole genome shotgun sequence.
ACCESSION   DAOSWW010000820 DAOSWW010000000
VERSION     DAOSWW010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85033_length_1398_cov_2.675354"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            566..1150
                     /locus_tag="PK442_13360"
     CDS             566..1150
                     /locus_tag="PK442_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88685.1"
                     /translation="MYRLFSYAMVLFWILVQQTCLLCARWACDDLSGRKKPLALFLAA
                     QALLFLIVSTAAVSDLAGWLPRLHEHTNNSPSMVWQFLCTVMLALDGGLIAYAWRFYR
                     LHVLKGNLPVDNALKLFLAWGTVCLLLYGAYFAPALSVATRHSLTLREWEYICLFYFR
                     IVNTCYLILEGAMGLVAFLLWRNLRKEGAPDAHC"
     gene            1137..>1398
                     /locus_tag="PK442_13365"
     CDS             1137..>1398
                     /locus_tag="PK442_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88686.1"
                     /translation="MPIVDRFFLWIARWEGIPGFLGSLEVWTNMLCSAADIVFVFWFL
                     RIAGIARAEQGRKAIRLRYFILAACLLLTPALFLFKGPVLMIW"
BASE COUNT          309 a          378 c          318 g          393 t
ORIGIN      
        1 tcccatcacc atccattcgt gctgtgatat agatcgaata aaaacctcgc ccagtgaaaa
       61 accggacgcc gacagacact cccgggcact gttcatcgtc ctgtaatcaa gtgcgatgaa
      121 ctcggcaaaa gccccccaag acttcgaaac actctttcct cgcagctttt catcctcccc
      181 cttccccaag gaaattcgac cgttacaatt caaaaaaaag cacatccgta gttcacggtt
      241 ttctgaacac agcttttcaa gaagctctct tcctcttgac ctctaattgt accaactttt
      301 ttctgttgta gagtacttga tttcgatatt tcaatgagaa aagacaaaaa aagcttcgga
      361 tatggctgag tcgtaaaaaa tccgcgctgt ttaaccaaca atctcggtgt cagctgaacg
      421 cttattacag gggactcttc ctgatattga caggagagca aggaaagtat atatataaaa
      481 acaatacaat aaaagctttt tatcagaaac gcaaattttc tttttatcgc acccgtgaag
      541 cgtttcttgt acgggaggtg cggatgtgta tcgcctgttc agctatgcta tggttctctt
      601 ctggatcctc gtacaacaaa cctgtcttct ctgcgcccgc tgggcgtgcg atgatctgag
      661 cggcagaaaa aaaccgcttg cactttttct tgcagctcag gcgcttcttt tcctcattgt
      721 atcgacggct gcggtcagcg atctcgccgg ttggctccct cgcctccatg agcatacaaa
      781 taactctccc tccatggtct ggcaattcct ctgcaccgtc atgctcgcgc tcgacggagg
      841 actcattgcg tacgcctggc gtttttaccg gttgcacgtt ctgaaaggaa atcttcccgt
      901 ggataacgct ctcaaactct tcctcgcctg gggaacagtg tgcctgctcc tctacggagc
      961 ctactttgcc ccggcacttt cagtcgctac acgtcattcg ctgactcttc gggaatggga
     1021 atacatctgt ctcttctatt tccggatcgt gaacacatgc tatctcattc tggagggagc
     1081 catggggctt gtcgcgtttt tgctgtggag aaacctgcgg aaggagggcg cccctgatgc
     1141 ccattgttga tcgttttttt ctgtggatcg ctcgatggga aggcatcccg ggctttttgg
     1201 gttcgctcga agtgtggacg aacatgctct gcagcgcggc ggacatcgtc ttcgtcttct
     1261 ggtttctgcg gatagccggc atcgcccgtg ccgagcaggg gcgcaaagca atccgtctcc
     1321 ggtattttat tcttgcagcc tgtttgctgc ttacccccgc gctcttttta ttcaaagggc
     1381 ctgtactgat gatatgga
//
LOCUS       DAOSWW010000821         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_85112_length_1397_cov_3.249627, whole genome shotgun sequence.
ACCESSION   DAOSWW010000821 DAOSWW010000000
VERSION     DAOSWW010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85112_length_1397_cov_3.249627"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /gene="alr"
                     /locus_tag="PK442_13370"
     CDS             <1..638
                     /gene="alr"
                     /locus_tag="PK442_13370"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOO88687.1"
                     /translation="HFATADDADLAYVHEQHALFLKALDLIAAKGISIPLRHCCNSGG
                     TLSFPQWAVDGVRPGQIVCGMYPTEHVPRTVPVLPGFSFKTAVAALRTLPPGQGVSYG
                     LTYVTRSEERLAVLPLGYADGFARPLSNRGEVLIRGTRCRVAGRICMDQCMVNVSHLP
                     EVSVGDEVVVIGPQGGDEISIYEYAEKLDTIVAAIGAMISKRVPRVYVEGK"
     gene            699..>1397
                     /locus_tag="PK442_13375"
     CDS             699..>1397
                     /locus_tag="PK442_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88688.1"
                     /translation="MQPIRVVVWGLGSMGSLMARILDEKEGVEIVGAADSHPEKTGRT
                     LGELFGLKRFPDVVVTDGATVPLPEADVALLAVSSFISGTEGYIRELVGSGTNVITIA
                     EEWTYPWRTAPETAAAIDGLAKAHGVTVLGTGVNPGFVLDTLIIALTGVCSDVRTVRA
                     RRVNDLSPFGPTVMETQGVGRTLDEFDAGRRDGSVVGHIGFVQSVSMIASSLGIPLDE
                     IRETIEPIVSRTHRR"
BASE COUNT          236 a          421 c          510 g          230 t
ORIGIN      
        1 cgcacttcgc cacggcggac gacgccgatc ttgcctacgt gcacgagcag cacgcgctct
       61 tcctgaaggc gctcgatctg atcgcggcga agggaatcag cattccgctg cgccactgct
      121 gcaactcggg agggacgctc tccttcccgc agtgggctgt ggacggcgtc cggccggggc
      181 agatcgtctg cgggatgtac cccaccgagc acgtcccccg cacggttccc gttcttccgg
      241 gcttctcgtt caagacggcc gtggcggccc tcaggacgct gccgccgggg cagggggtca
      301 gctacgggct gacgtacgtc acgcgctccg aggagcggct cgccgtcctc ccgctggggt
      361 acgccgacgg cttcgcccgc ccgctctcga acaggggaga ggtgctgatc cgggggacga
      421 gatgccgggt ggcggggcgc atctgcatgg atcagtgcat ggtgaacgtc tcgcacctgc
      481 cggaagtctc cgtgggcgac gaggtcgtcg tcatcgggcc gcagggcggc gacgagatct
      541 ccatctacga atacgccgaa aagctggaca ccatcgtggc ggccatcggc gcgatgataa
      601 gcaagcgagt tccccgcgtc tacgtcgagg gaaagtgata atgccccgct ccgtccggaa
      661 ctgtccggcc ggggcggttt tttctaaggg ggaagacgat gcagccgatc agagttgttg
      721 tgtgggggtt ggggagcatg gggtcgctca tggcgcgcat cctcgacgag aaggagggcg
      781 tggagatcgt cggcgcggcg gattcgcacc ctgagaagac agggagaacg ctgggggagc
      841 tgttcggcct gaagcgtttt ccggacgtcg tcgtgacgga cggcgccacc gttccgcttc
      901 cggaagccga cgtcgcgctg ctcgccgtat cgtcgttcat cagcgggacc gaagggtata
      961 tacgggagct tgtggggagc ggaacgaacg tgatcaccat cgcggaggag tggacgtacc
     1021 cgtggaggac ggcgccggag acggcggccg caatcgacgg actcgcgaaa gcccacgggg
     1081 tgacggtgct gggaacgggg gtcaaccccg gattcgtgct ggacacgctg atcatcgcgc
     1141 ttacgggcgt ctgcagcgac gtccggacgg tgcgcgcgcg gcgcgtgaac gatctctcgc
     1201 cgttcggccc gacggtgatg gagacgcagg gggtgggcag aacactcgac gaattcgacg
     1261 ccggaaggcg tgacggaagc gtcgtcgggc acatcggctt cgtgcagtcg gtgagcatga
     1321 tcgcctcgtc gctggggatt ccgcttgacg aaatcaggga gaccatcgag cccatcgtct
     1381 cgcggacaca ccgccgg
//
LOCUS       DAOSWW010000822         1395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_85303_length_1395_cov_3.557463, whole genome shotgun sequence.
ACCESSION   DAOSWW010000822 DAOSWW010000000
VERSION     DAOSWW010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85303_length_1395_cov_3.557463"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1395)
                     /locus_tag="PK442_13380"
     CDS             complement(<1..>1395)
                     /locus_tag="PK442_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88689.1"
                     /translation="TERAALSISLSGEQSDRGRLSSGMAVVGGVVLTVLGRKLVQRMM
                     HLAVVKIGGKVLSRMIPIIGVGLLLYEGIQMGQAKEAFEEELRKSFFTEYTADVTVES
                     VWNASPDGSLPSMKQEMERNINTLLSDWERICRSEAVSMIHSAQVLASSEHVRNYVSG
                     ELEKGADFQLLLQKINALWDAFGQSLALEPVEFFESILIDSPDRNDLRLLSRDPGSRF
                     IELYKEWGAEFLKAVNKIGVENYVSTEWKTSDVNWSVLNRSLDFLPDISGNRNAARGL
                     LFLIQEGVPLDGFSPDLMAKIAVKKDVFLKVWQALTPDSRKLAVIFESGRPLVILEPS
                     LIAFPEACSLFLRDYGVEFWTSWSSDDIFDLLRISEIRGKNSGKLRAASVLPEERADL
                     LEIYRRSGERGIILWDIHTKEPTGEIGRKLARQSIACMAEGYPFEDMKDPEFVRFAAA
                     TLNFPAGRFFYDTLK"
BASE COUNT          296 a          412 c          387 g          300 t
ORIGIN      
        1 cttgagggtg tcgtagaaaa atcgccctgc cggaaagttg agtgtggctg cggcgaaacg
       61 gacaaactca ggatccttca tgtcctcgaa ggggtacccc tcggccatgc aggcgatact
      121 ctgacgggcg agctttctgc cgatttctcc tgtaggttcc ttcgtatgga tgtcccacag
      181 aataattccc ctctccccgg aacgccggta gatttcgagc agatccgcgc gttcctccgg
      241 aaggacggaa gccgctcgta gtttcccgct gttctttccg cgaatttccg aaatcctgag
      301 gagatcgaag atatcgtcgc tcgaccagga ggtccagaac tccaccccgt agtcgcgaag
      361 gaagagcgag caggcttcgg gaaaggcgat gagggacggc tcaagaataa cgaggggacg
      421 tccgctctcg aagatgaccg cgagctttct ggagtcagga gtgagcgcct gccatacttt
      481 caggaagaca tccttcttta cggcgatctt cgccatcagg tccggagaaa agccgtcgag
      541 aggaaccccc tcctggatca aaaagagcag gccgcgcgcg gcgttccggt tgccgctgat
      601 gtccggaaga aaatcaaggc ttctgttcag tacgctccag ttgacgtcgc tcgtcttcca
      661 ctcggtggat acgtagttct ccaccccgat cttgttgacc gctttcagaa attccgctcc
      721 ccactctttg tacagctcga taaacctgct gcctgggtca cgcgagagca gacgcagatc
      781 gtttctgtcg ggggaatcaa tgagaatcga ctcgaaaaat tcgacgggct ccagggcgag
      841 cgactgtccg aacgcatccc aaagggcgtt gatcttctga agcagcagct ggaagtcggc
      901 gcccttctcg agttctccgc ttacgtagtt cctgacatgt tccgaggaag cgagcacctg
      961 cgcgctgtga atcatcgaga cggcctcgga acggcagatc cgctcccagt cgctcagcag
     1021 cgtgttgata ttccgttcca tctcctgttt catggaggga agagaaccgt cgggactcgc
     1081 gttccatacg gactcgacag tgacgtcggc ggtgtactcc gtaaagaagc tcttcctgag
     1141 ttcctcttcg aacgcttcct tcgcctgtcc catctggatt ccctcataaa gaagcaggcc
     1201 gaccccgatg atggggatca tccgcgagag tactttacct ccgatcttga ccactgccag
     1261 gtgcatcatt ctctggacga gcttccgccc gagaacggtg agcactacgc cgccgactac
     1321 ggccataccg gacgagagac gccccctgtc gctttgttcc ccggaaaggg agatagagag
     1381 cgcagccctc tccgt
//
LOCUS       DAOSWW010000823         1394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_85385_length_1394_cov_4.097087, whole genome shotgun sequence.
ACCESSION   DAOSWW010000823 DAOSWW010000000
VERSION     DAOSWW010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85385_length_1394_cov_4.097087"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1275
                     /locus_tag="PK442_13385"
     CDS             169..1275
                     /locus_tag="PK442_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOO88690.1"
                     /translation="MKVRDFLLERYFAKYEFSVRHMLSASDCETISVRELLEGADADV
                     EELWSELRLSYTESRGHPALLREIARETGVSADEAIVLNPEEGIFLTLNALLDSDDHV
                     VVLSPCYQSLYELPKSLGCEVTRWYLKKEDSVWKLDLPFLERAIKENTRLVIVNFPHN
                     PTGFLPSVEQLNAIAELSVKKGFFVFSDEMYRGLEHNPADRLPSAPSIIRRSVALGGL
                     SKAYGLPGLRTGWVTSTDKWLLDRIAALKDYTTICTSAPSEILAIAALRKSDELTGRC
                     RGIVKENLEMARSFFQENSGRFEWFEPHGSSVAFPRLLGGQPVKDFCERAVTERSLLV
                     LPDVVFDVDWNHFRIGLGRRSFPEALSVLREMLN"
BASE COUNT          296 a          380 c          406 g          312 t
ORIGIN      
        1 agagagccgg gggctcgcgc tgaagaagta ggcggatcag aggagaagag caaaaaggac
       61 gccctcgggc gtcctttttt ataggttcct ctcttccgga agggcgtgat ggcgggtata
      121 atacaaaaga aaacgtctat ataaaatctc ttttaaggct gtgatgcaat gaaagttcgg
      181 gattttctcc ttgaacgata tttcgccaaa tacgaattct cggtacgtca tatgctcagc
      241 gcgtccgact gcgagacgat aagcgtccgc gagcttcttg agggggccga tgccgacgta
      301 gaggaactct ggagcgagct tcgtctttcg tacacggagt cgagggggca tccggcgctc
      361 ctccgcgaga ttgcccgcga aacgggagtg tcggccgacg aggcgatcgt cctcaatcct
      421 gaagagggga tcttcctgac gctcaacgcg ctgctggaca gcgacgacca tgtcgtcgtg
      481 ctcagtccgt gctatcagtc gctctacgaa cttcctaaat cgttggggtg cgaagtgacc
      541 cgatggtacc tcaagaagga ggacagtgta tggaagctgg atctcccctt ccttgaaagg
      601 gcgatcaagg agaatacgcg ccttgtcatc gtcaacttcc cgcataaccc gaccggtttt
      661 cttccgtccg tggagcagct gaacgccatc gcggaacttt cggtgaagaa gggattcttc
      721 gtcttctccg acgagatgta ccgggggctt gagcacaacc ctgcggatcg tcttccctca
      781 gcgccgtcaa tcattcgcag aagcgtcgcc ctgggcggtc tctccaaggc gtacggcctg
      841 ccggggctgc ggaccggatg ggttacgtcg acggacaagt ggcttctgga caggatcgcc
      901 gccttgaagg actacaccac gatctgcact tcggcgccct ctgaaatcct cgcaatcgcc
      961 gctcttcgca agagcgatga gcttaccggt cgctgccgcg gcatcgtaaa ggagaatctt
     1021 gagatggcgc gctccttttt ccaggaaaac agcgggcggt tcgagtggtt cgaacctcac
     1081 ggaagctcgg tcgccttccc ccgccttctc ggcggacaac cggtgaagga tttctgcgaa
     1141 cgcgccgtga ccgagcgtag tctgttggta ttgcccgacg tcgtcttcga cgttgattgg
     1201 aaccatttca ggataggcct ggggagacgc tcgttccccg aggctctctc tgttctgcgc
     1261 gagatgctca actgattttt cccaaagccc cccgccttga gcaaggggct ttcttcggaa
     1321 tcgttgcatt ccgcttgtaa catgggacga tgcataattg taaaaaataa cggacgagtt
     1381 ttaacgtgaa gcaa
//
LOCUS       DAOSWW010000824         1394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_85413_length_1394_cov_2.892457, whole genome shotgun sequence.
ACCESSION   DAOSWW010000824 DAOSWW010000000
VERSION     DAOSWW010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85413_length_1394_cov_2.892457"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PK442_13390"
     CDS             <1..559
                     /locus_tag="PK442_13390"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO88691.1"
                     /translation="LPYFPLKARRLVAAELQKIGVEVSERRTTLVSGDTLVYSDGSTG
                     DADFLVVATGARPSKVLTESGLEVDDEGAVIVDEYLQSLSAKGVFGGGDCVAFQPMEL
                     ERSASHALRQGRVLFANLLAYLSGEPMRPFIPKKEPLTILNLGRTGLLVRKPFVARGR
                     FALLLKRWIDRRIVKEFQSPSEEKS"
     gene            619..>1394
                     /locus_tag="PK442_13395"
     CDS             619..>1394
                     /locus_tag="PK442_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOO88692.1"
                     /translation="MERLNIRKLLTQFGELVKAEWSTFWYATLGAVLMSFSIIALIVP
                     YRFAGAGLAGIALLARYVFDVSPAWVLAVGNLLLLGWGWRALSARFVLWTLYVSILSS
                     FAVAFFELFHYPVLANTLLAALLGGVCSGIGMGLVFRVGGSTGGTDVIVAVAKKRWGV
                     DMGMFSFYINTAILFLSWFAVDLEQLLMGGVLLYAESITIDSVLKSFDRRKQVTVITS
                     KMEEVKRFIVEDMEKGATVIRGEGAYTAAERPMIMVVLNRR"
BASE COUNT          246 a          399 c          435 g          314 t
ORIGIN      
        1 attgccgtac ttccccctga aagcgcgccg gcttgtggcg gccgagctcc agaagattgg
       61 ggtcgaggtt tcggaacggc gcaccacgct cgtatccggc gatacgctcg tctactcgga
      121 cggttcgacc ggagacgccg attttctggt ggtcgcaacg ggcgcgcgtc cgtcgaaagt
      181 gctgacggaa tcagggcttg aggttgacga cgagggcgcc gtgattgtcg acgagtacct
      241 ccagtcgctt tcggcgaagg gcgtgttcgg cggaggagac tgcgttgcct ttcagcccat
      301 ggagctggag cgttccgctt cccatgccct ccggcagggg cgggtgctct tcgcgaatct
      361 tttggcgtac ctctcagggg agcctatgcg tccgtttatt ccgaagaaag agccgctcac
      421 gatcctgaat cttggaagaa cggggctgct tgtcaggaag ccgttcgtcg ccagaggtcg
      481 cttcgcgctt ctgttgaagc gatggatcga ccgccgtatt gtgaaggagt ttcagagtcc
      541 ctccgaggaa aaatcgtgag acgaacgatg aaagcctttt gcgtaccctc aatttctctc
      601 agttgaaagg aatgaatcat ggagcgtttg aacatacgaa agctcttgac gcagttcggc
      661 gaactggtca aagcggagtg gagcaccttc tggtacgcaa cgcttggcgc ggtgctgatg
      721 agtttctcca tcatcgccct catcgttccg taccgtttcg cgggcgccgg cctcgcagga
      781 atcgctctgc tcgcacggta cgtcttcgat gtctcccccg cgtgggtgct cgccgtcggc
      841 aacctgctgc tgctgggctg ggggtggagg gcgctctccg cgcgctttgt cctctggacg
      901 ctctacgtct cgatactctc ctcgttcgcc gtcgcctttt ttgaactctt ccactacccc
      961 gtgctggcca acaccctcct tgctgcgctg ctgggcgggg tgtgcagcgg aatcggcatg
     1021 gggctggtct tccgcgtcgg aggctccacc ggcggaaccg acgtcatcgt cgccgttgcg
     1081 aagaagcgct ggggcgtcga catgggaatg ttttccttct atataaatac tgcgattctc
     1141 ttcctttcat ggttcgccgt ggacctcgaa cagctcctca tgggaggagt gctgctctac
     1201 gcggagagca tcaccatcga cagcgtgctc aagtccttcg accggagaaa gcaggttacc
     1261 gtcatcacat cgaagatgga ggaggtcaaa cgctttatcg tcgaggacat ggagaaaggc
     1321 gccacggtca tcaggggcga aggagcctac acggctgcgg agcgcccgat gatcatggtg
     1381 gtgctcaacc ggcg
//
LOCUS       DAOSWW010000825         1393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_85501_length_1393_cov_3.168909, whole genome shotgun sequence.
ACCESSION   DAOSWW010000825 DAOSWW010000000
VERSION     DAOSWW010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85501_length_1393_cov_3.168909"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK442_13400"
     CDS             complement(<1..353)
                     /locus_tag="PK442_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO88693.1"
                     /translation="MRVQLHAAAEKWLKEYIDSFRDENGGLHPLMEVKRTHSLRVRDN
                     ASQIASELGWSGEQLDCALTAALLHDTGRFPQFAEWGTYYDGASLDHGDLGEKTLRKH
                     FPDEKVATYGRWEDIL"
     gene            496..>1393
                     /locus_tag="PK442_13405"
     CDS             496..>1393
                     /locus_tag="PK442_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOO88694.1"
                     /translation="MHEIKNRVFSMLSLLSLLLFFAVLPATEATGWQGVLHWSYDALS
                     SVSGSPAVAGDKILIGDDTGKLHALDRNTGRSLWIFDTKATINGAPAVFGGSAYVGGA
                     DGTLFAINLASGAVQWSYSPSEKYTPASIWGSPAAGDGRVFFNSADGKTYALNATNGA
                     LAWTFDTGRELRSSPAYSDGLVFVGAYEGLFHALNAQTGKKEWGGGSGGPIGSSATVS
                     NGVVYFGSWDGQLQAVRIKGVIPLWKYNAGESITTSPTVSGGKVFFGAINGKLVAVDT
                     ASGKLAWEFPVPGGIHSDPAVHE"
BASE COUNT          289 a          423 c          387 g          294 t
ORIGIN      
        1 aggatatcct cccatcgccc gtacgtcgcg accttctcat ccggaaaatg cttcctgagc
       61 gtcttttccc ccaagtcgcc atgatccaga gatgcgccgt cgtaataggt tccccactcc
      121 gcgaattggg ggaaacgacc agtatcgtgc agtaacgccg ccgtgagcgc acaatcaagc
      181 tgctccccgg accacccgag ctcggaggca atttgcgacg cgttgtcccg cacgcggaga
      241 ctgtgcgtcc gtttcacctc catcaagggg tgcaggcccc cgttttcatc ccggaatgaa
      301 tcgatatact ctttcaacca cttttccgcc gcggcgtgaa gttgcacgcg caccacgctc
      361 ccttctcttc aacgcaccga attgtacccc tgtttccctg tcttttcaac gtcggtattt
      421 taacgatgca acaacaccga agtagggtat aatatcagct agaagtactc taccccaaga
      481 aaagaggtgt tgttcatgca cgaaataaaa aacagagttt tttcgatgct gtctctcctt
      541 tcgctccttc ttttctttgc cgttcttccg gcgactgaag cgactgggtg gcagggcgtt
      601 cttcactgga gctacgacgc gttgtcctcc gtctccggct cccccgccgt cgcgggggac
      661 aagattctca tcggcgacga tactggcaaa ctgcacgctc tggatagaaa tacaggacgc
      721 tctctctgga tcttcgacac gaaagcaacg ataaacggcg cgcccgctgt gtttggagga
      781 agcgcgtatg tcggcggggc cgacggcacg ctcttcgcca tcaatcttgc atccggagcg
      841 gtgcagtgga gttacagccc ctccgagaag tacacccccg cttcgatatg gggctccccc
      901 gccgcaggag acggaagagt cttcttcaat agcgcggacg ggaaaacgta cgccttgaac
      961 gcgacgaacg gcgcgctcgc gtggacgttc gacacgggaa gagagctccg ttcttcgccg
     1021 gcctacagcg acggtcttgt cttcgtcggc gcgtacgagg ggctcttcca tgccctgaac
     1081 gctcagaccg gaaagaagga gtggggcggc ggcagcggcg ggcccatagg ctcttcggcg
     1141 acggtgtcga acggcgttgt ctacttcggc agctgggacg gccaactgca ggcagtccga
     1201 atcaagggag tcattcctct ctggaaatac aacgcagggg aatcgatcac aacatcccct
     1261 acagtatcgg gggggaaagt tttcttcggc gccatcaacg gcaaactcgt tgcagtggac
     1321 acggcgtccg gaaagcttgc gtgggaattc ccggtaccgg gcggcattca ctccgacccg
     1381 gcagtgcatg aag
//
LOCUS       DAOSWW010000826         1392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_85614_length_1392_cov_2.792072, whole genome shotgun sequence.
ACCESSION   DAOSWW010000826 DAOSWW010000000
VERSION     DAOSWW010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85614_length_1392_cov_2.792072"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..415
                     /locus_tag="PK442_13410"
     CDS             65..415
                     /locus_tag="PK442_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HOO88695.1"
                     /translation="MTERTESQVLETNEYGFVEDPRYKQCEKEAWYGIILGIVNLVIW
                     PVAGYGLGSGPVEEYSYIMGFPAWFFISCIANSVFAVVATIYLVTRKMRDMPLDPMTK
                     EEAEEFVRNGGKAR"
     gene            412..1233
                     /locus_tag="PK442_13415"
     CDS             412..1233
                     /locus_tag="PK442_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88696.1"
                     /translation="MSTIQWSTLLPIVCYFALMYFIGFYALKLVNNASLRKGGSGAAY
                     MEEYMTGGRGTGGFVLAMTLVATYLSAGSFIGGPGTAYTHGLAWVFLAMSQIPTGYYT
                     LMVLGKKFAIVSRKTGANSISDFLRARYESNAVLIIASLSIIFFLIAAMAAQLIGASR
                     LLQGSTGLDYTTALIFFAITVVVHTAVGGFRGVVLNDTLQGVVMTVSTAALFIAIVMK
                     GGGIANIVQSMKAINPGMISPYGVKEGFMTVPWSPASGCSSASRLSAFPPSRSAP"
     gene            1179..>1392
                     /gene="panF"
                     /locus_tag="PK442_13420"
     CDS             1179..>1392
                     /gene="panF"
                     /locus_tag="PK442_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="mediates high affinitiy panthothenate transport
                     which is stimulated by the presence of sodium ions; member
                     of SSS family of sodium/solute symporters; the imported
                     panthothenate is phosphorylated by panthothenate kinase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/panthothenate symporter"
                     /protein_id="HOO88697.1"
                     /translation="MLVGFAIIGLPAVAQRAMSYKSSKSLHSGIIYGSIVSLVLLMGM
                     HLAGAFGVTQVSGIASGDLVIPTLITN"
BASE COUNT          273 a          411 c          414 g          294 t
ORIGIN      
        1 gtgaatctgg agaagttgta ctaggcatcg gttagcagaa gcaggaagga ggtcgtggaa
       61 gaccatgacg gaaagaacgg aatcgcaggt tcttgaaacg aacgaatacg gctttgtcga
      121 agaccctcgt tacaaacagt gtgaaaaaga ggcctggtac ggcatcatac tcgggatcgt
      181 caacctcgtt atttggcccg ttgcaggcta cggactcggg agcggtccgg tggaggagta
      241 ctcctacatc atgggcttcc cggcgtggtt cttcatcagc tgcatcgcaa actccgtgtt
      301 cgcggtcgtc gcgaccatct atctcgtcac gaggaagatg cgcgatatgc ccctcgatcc
      361 gatgacgaaa gaggaggccg aggagttcgt tcggaatgga gggaaagcac gatgagcacg
      421 atccaatggt cgacgcttct gcctattgtc tgctacttcg cgctgatgta cttcatcggc
      481 ttctatgccc tgaagctggt gaacaacgcc agtctccgca agggaggaag cggcgccgcc
      541 tacatggaag agtacatgac cggcggacgc ggcacgggag gtttcgtgct cgcaatgacg
      601 cttgtcgcca cctacctgag cgccggcagc ttcatcggcg gaccggggac ggcgtacacc
      661 cacgggcttg cctgggtctt cctcgccatg tcgcagatcc ccaccgggta ctacacgctg
      721 atggtgctgg ggaagaagtt cgccatcgtc tcgcggaaga cgggcgccaa ctcgatcagc
      781 gacttcctgc gcgcccggta cgagagcaac gcggtgctga tcatcgcctc cctctccatc
      841 atattcttcc tcatcgcggc catggcagcc cagctgatcg gcgcctcccg cctgctgcag
      901 ggctcgacgg ggcttgatta cacgaccgct cttattttct tcgccatcac cgtcgtggtg
      961 catacggcgg tcggaggatt ccggggtgtc gtcctgaacg acacgcttca gggcgtcgtc
     1021 atgaccgtgt ccaccgcggc gctcttcatc gccatcgtca tgaagggcgg cggaatcgcg
     1081 aacatcgttc agtcgatgaa ggcgatcaac cccggcatga tctctcccta cggagtgaag
     1141 gaagggttta tgaccgtccc gtggtctcca gcttctgggt gctcgtcggc ttcgcgatta
     1201 tcggccttcc cgccgtcgcg cagcgcgcca tgagctacaa gagctcgaag agcctgcact
     1261 cgggaatcat ctatggaagc atcgtctctc tcgtgctttt gatgggaatg caccttgccg
     1321 gagccttcgg cgtcactcag gtctccggaa tcgcatcggg cgacctggtc attcccacgt
     1381 tgatcaccaa cc
//
LOCUS       DAOSWW010000827         1391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_85697_length_1391_cov_4.620509, whole genome shotgun sequence.
ACCESSION   DAOSWW010000827 DAOSWW010000000
VERSION     DAOSWW010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85697_length_1391_cov_4.620509"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PK442_13425"
     CDS             <1..591
                     /locus_tag="PK442_13425"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOO88698.1"
                     /translation="LPHAGSLAGSPADRIIPLLRSPNIVETSVGLATLNALIQQDRYD
                     EDSSNDDLVQLLNITSNDRVGMVGDITPIFNRIQKHAKECIVFDEGKSAWEGITDTSL
                     EKEMLPRCDVALLSATTLLNNTFESVLSMVAGAREICVIGPSAPLLPEVFKKRGVTLL
                     SGRRFLDADRLLRIVSEAGGTKCFGPVSLKVNIQLK"
     gene            complement(713..>1391)
                     /locus_tag="PK442_13430"
     CDS             complement(713..>1391)
                     /locus_tag="PK442_13430"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HOO88699.1"
                     /translation="YYRQANLAVLGTSEESPARRSLDVAYSDVEAAFDAYMTFWNKGR
                     PFILAGHSQGSEHLLHLLERRFGDAELRKKLVAAYIIGWSVTKDDLARYPFLKIAESP
                     DQTGSIVSFNTQGPNPGYSIVREGAVGVNPLTMTITGEVADADQNLGAVFFPDKGVME
                     IPHYTGGQTVGGAFVIPTPSNINDLDAMKIPEFYHPYDYTFFYRNLQQNAELRTRTYL
                     ESHGIKN"
BASE COUNT          311 a          398 c          382 g          300 t
ORIGIN      
        1 ctgccacacg ccggttccct tgctggaagc cccgcggacc ggattattcc tctcctgcgt
       61 tctccgaaca tcgtggagac atccgtcgga ctggcgaccc tcaacgcgct tatccagcag
      121 gacaggtacg atgaagactc ttccaacgac gatcttgtcc aactgctcaa catcacctcg
      181 aacgaccggg tgggaatggt cggcgatatt acgcccattt tcaacaggat tcaaaaacac
      241 gcgaaagaat gcatcgtctt cgacgaaggg aaaagcgcct gggaggggat taccgacacg
      301 tcgcttgaaa aggagatgct tccacgctgc gacgtcgctc ttctttccgc gacgacgctc
      361 ctcaacaaca cgttcgagag cgtgctctcg atggtggccg gagcgcggga aatctgcgtc
      421 atcggtcctt cggcgcccct gctgcccgaa gtgttcaaaa aacggggcgt gacactgctt
      481 tccggacgcc gttttctcga cgccgaccgg ctgcttcgca tcgtcagcga ggccggggga
      541 accaagtgct tcggcccggt cagcctgaaa gtcaatatcc agctgaaata gaagaaaact
      601 gctccactgg cgaagcgcct tcttaaaatt acaatacaaa gtttttcaaa agggaagcgg
      661 cccgggagta aacggggaaa aaaagttcaa ctcccgggcc gccggaggat tgtcagtttt
      721 ttatgccatg cgattccagg tacgtacgtg tccgaagttc ggcgttttgc tgaagattcc
      781 tgtagaagaa cgtatagtcg taagggtggt aaaactccgg aattttcatg gcgtcaaggt
      841 cgttgatgtt ggagggcgtc gggatgacga aagccccgcc gacggtctgc cctcctgtat
      901 agtggggaat ctccataact cccttgtcgg gaaaaaatac cgcccccaag ttctgatccg
      961 cgtccgcaac ctccccggtg atggtcatgg tgagcggatt gacgcccacg gccccttccc
     1021 gaacgatgct gtacccggga ttgggtcctt gtgtgttgaa gctgacgatg ctcccagtct
     1081 ggtccgggct ctcggctatc tttaaaaaag gatatcgggc aaggtcgtct ttcgtgaccg
     1141 accagccgat gatataggcc gcaaccagct tcttcctcag ttcagcatcg ccgaagcgcc
     1201 gttccaaaag atgcagcagg tgttccgatc cttgactgtg acctgccagg ataaagggcc
     1261 tccccttgtt ccaaaaagtc atgtaggcgt cgaaggcagc ctccacgtcc gagtaggcca
     1321 cgtcaaggga ccttctggcc gggctctcct cagatgtgcc cagcacagcc agattcgcct
     1381 gacggtagta t
//
LOCUS       DAOSWW010000828         1384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_86436_length_1384_cov_3.043642, whole genome shotgun sequence.
ACCESSION   DAOSWW010000828 DAOSWW010000000
VERSION     DAOSWW010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86436_length_1384_cov_3.043642"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK442_13435"
     CDS             <1..389
                     /locus_tag="PK442_13435"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOO88700.1"
                     /translation="EDAQSLVRHMTGATETEEQGFSEMGLSVLHEVGNIMIGSYITAL
                     SDFLGFELNPSVPSLAMDMAGAILAFSLSEIGRSGDTAIVIDGALSLDGGALSGDGAG
                     HIFLLPDPESFNKIFQALGVAGNGDH"
     gene            376..864
                     /locus_tag="PK442_13440"
     CDS             376..864
                     /locus_tag="PK442_13440"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOO88701.1"
                     /translation="MATIRVGIAESGVVLNPNSVSTLGLGSCVGVTLYDERRKIGGMV
                     HVMLPSTELAKGADFNRSKFADSAVPDLLQQMIRSGADQRRLVAKLAGGAQMFNLGGD
                     ADSLLKIGARNVEACKKALSLLRIPIVAEDTGGSWGRTVELFSESGVLEIRAIGKAKT
                     HI"
     gene            886..>1384
                     /locus_tag="PK442_13445"
     CDS             886..>1384
                     /locus_tag="PK442_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOO88702.1"
                     /translation="MNTDKHLLGTENVGKLLLRLSLPSVIGMLVMASYNIADSIFIGR
                     GVGPLGLASVMICFPIQFLGGALAIMAGVGGASVVSRSLGAGDTDRAQQAFGATAAFS
                     LSVGLLVSSLSLFFLPSLLRLFGATEGILPYAEDYLSVILFGVPFQMFAMAGNHMARA
                     EGRARI"
BASE COUNT          235 a          433 c          430 g          286 t
ORIGIN      
        1 gggaggacgc gcaatcgctc gtccggcaca tgacgggagc gaccgagacc gaagagcagg
       61 gcttctccga gatggggctc tccgtcctcc acgaggtggg gaacatcatg atcggctcgt
      121 acattacggc gctctccgac ttcctcggct tcgagctgaa cccgtccgtt ccgtcgctgg
      181 cgatggacat ggcgggagca attctcgcgt tcagcctctc ggagatcggg cgaagcggcg
      241 acacggcaat cgtgatcgac ggcgcgctct cgctggacgg cggcgcgctt tccggggacg
      301 gagcggggca tatcttcctg ctccccgatc cggaatcgtt caataaaatc ttccaggctc
      361 tgggggtcgc cggaaatggc gaccattagg gtgggcatcg ccgagtcggg ggtcgtactg
      421 aacccgaaca gcgtctcgac cctgggcttg ggctcgtgcg tcggcgttac cctctacgac
      481 gaaagaagaa aaatcggcgg tatggtccat gtgatgcttc cttccacgga actcgccaag
      541 ggcgcggact tcaacaggtc gaagttcgcc gattccgccg tccccgacct gctgcagcag
      601 atgatccgct ccggcgcgga ccagagacgg ctcgtggcca agctggcggg cggcgcgcag
      661 atgttcaacc tcggcggcga tgcggactcc ttgctgaaaa taggcgcccg gaacgtggaa
      721 gcctgtaaaa aagcgctctc tcttcttcgc atccctattg tcgccgagga tacgggcggc
      781 tcgtggggac gcaccgtgga actgttctcc gagtcgggag tcctcgaaat ccgcgctatc
      841 ggcaaggcaa aaacacatat ttgaacaaag attcggaggc atctcgtgaa cactgataaa
      901 catctgctcg gcacggagaa cgttggcaag ctattgctgc gcctttccct gccttcggtc
      961 atcggcatgc ttgtcatggc ctcgtacaat atcgcggact ccattttcat cggtcgcggc
     1021 gtcggcccgc tcggcctcgc ctcggtgatg atctgttttc ccatccagtt tctggggggc
     1081 gcgctcgcca tcatggcggg ggtagggggc gcctccgtcg tttcgcggag cctcggcgcg
     1141 ggagataccg accgcgcgca gcaagccttc ggcgcaaccg ccgcattctc tctttcggta
     1201 ggactgctcg tctcttcgct ctccctgttt tttcttcctt cgctgctccg cctcttcgga
     1261 gcgacggagg gcattctccc ctacgcggag gactacctct cggtcattct cttcggcgtg
     1321 ccgttccaga tgttcgccat ggcgggcaac cacatggcgc gcgccgaagg ccgggcgcgt
     1381 atcg
//
LOCUS       DAOSWW010000829         1382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_86644_length_1382_cov_2.989450, whole genome shotgun sequence.
ACCESSION   DAOSWW010000829 DAOSWW010000000
VERSION     DAOSWW010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86644_length_1382_cov_2.989450"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1382)
                     /locus_tag="PK442_13450"
     CDS             complement(<1..>1382)
                     /locus_tag="PK442_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOO88703.1"
                     /translation="DGNYALFEEWIAAEKERLPVICRGLAKLMVVEVALDRSQDNPQL
                     IFESMNSTGKELSQADLIRNFILMGLEQRLQTALYEKYWRPMEVDFGQEAYTTYFDAF
                     MRHYLTVRTGDLPRQGEVYEAFKLYARSDGIVQQGVERLVEDIRLYARFFCAMAFDAE
                     KDSDLQRAFHDLRTLKVDVAYPFLLELYARYHEGLLPKKNFLELIRLVESYVFRRAIC
                     SIPTNSLNKTFATFAASAKGLEPDRLFESLKAQFLLLPSYRRFPSDEEFRRDFKTRDL
                     YNFRSRSYWLRKLENHERKEHVKTGEYTIEHIMPQNENLSAEWRADLGTEWERVREEW
                     LHTLGNLTLTGYNSEYSDRPFREKRDMDGGFKDSPLKLNKGLGALEKWDEETIKKRAE
                     NLSAMALNIWKAPVLPKETLDSYRTQTARTVSYTIDDHPHLSPKSGNGGSKTRAMFEI
                     LRKEILSLDP"
BASE COUNT          263 a          388 c          396 g          335 t
ORIGIN      
        1 aggggtcgag agagagtatc tccttccgca gtatctcgaa catggcgcgt gttttcgagc
       61 cgccgtttcc gctcttgggg gaaagatggg ggtgatcgtc gatggtgtag gaaaccgtgc
      121 gcgcggtttg ggtccggtag gagtccagcg tctccttcgg caatacgggc gccttccata
      181 tgttgagcgc catggcgctc agattttcgg cccttttctt tattgtctct tcgtcccact
      241 tttcgagagc gccaagcccc ttgttgagct tcagcggact gtctttgaag cctccgtcca
      301 tgtcgcgttt ctcacggaaa gggcggtcgc tgtactcgga gttgtaccct gtaagcgtca
      361 ggtttcccaa ggtatgcagc cattcctccc gaactctctc ccactccgta ccaagatcgg
      421 ctctccactc ggcggagagg ttctcgttct gcggcatgat gtgttctatg gtgtactctc
      481 ctgtcttcac atgctccttc cgttcgtgat tctcaagctt tctcagccag tagctgcggc
      541 tccggaagtt atagaggtcg cgcgtcttaa aatcgcggcg gaattcctcg tcgctcggga
      601 accgtctata agaagggagc agaagaaact gcgctttgag gctctcgaaa aggcgatccg
      661 gctcaagccc tttcgcagag gcggcgaagg ttgcgaacgt cttattgagc gagtttgtgg
      721 ggatggaaca gatggcgcgc cggaaaacgt acgactccac cagccggata agttccagga
      781 agtttttttt cggcagcaac ccttcatgat agcgggcata cagctccagc agaaaggggt
      841 atgccacgtc gaccttgagc gtcctgaggt catggaacgc cctctgcaga tcggaatcct
      901 tctctgcgtc gaacgccatg gcgcagaaga atcgggcgta aaggcgaatg tcctctacaa
      961 gacgttcgac tccctgctgg acgattccgt cggagcgcgc gtagagtttg aaagcctcgt
     1021 agacttctcc ctgtctcggc aggtcgccgg tacgcacggt cagataatgt cgcatgaagg
     1081 cgtcgaagta ggtggtgtac gcctcctgac caaagtcgac ctccatcggt cgccagtact
     1141 tttcgtacag tgcggtctgg aggcgctgct ccagacccat caggataaaa ttccgtatga
     1201 ggtccgcctg gctgagttcc ttccccgtgg agttcatgct ctcgaagatg agctgcgggt
     1261 tatcctggct gcgatccagg gccacctcga cgaccatgag cttggcgagt cctctgcaga
     1321 taacaggcag cctctccttc tccgcggcga tccactcttc gaagagggcg taattcccgt
     1381 cg
//
LOCUS       DAOSWW010000830         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_86929_length_1379_cov_3.645770, whole genome shotgun sequence.
ACCESSION   DAOSWW010000830 DAOSWW010000000
VERSION     DAOSWW010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86929_length_1379_cov_3.645770"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PK442_13455"
     CDS             <1..553
                     /locus_tag="PK442_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO88704.1"
                     /translation="EGVFHRERGRGTFVSYPKPTCTSSSLTGFSENIRAAGLDAMTHI
                     LAFDEEEASRTIRESLQLPQHDPIVFNILRLRYVEGEPFSLENAWVPKCRFPELSRDL
                     LEGRSLYTLMREHFGMKLSHASQTIEPVFCSDFEAKNLDLDSGTPVLLFRRVAYAEPQ
                     TPVEYSKCVYRGKRFKYEISFGI"
     gene            601..>1379
                     /locus_tag="PK442_13460"
     CDS             601..>1379
                     /locus_tag="PK442_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOO88705.1"
                     /translation="MLKGLQKLGKALMLPVAVLPAAALLLRLGASDVLDIPFIMQAGA
                     AIFDNLALLFAVGIAVGVAFDGGGAAALAGAVGYFTLTKAVVTINSTINMGVLAGIVS
                     GIVAGMLYNKYHNIKLPDFLGFFGGKRFVPIAASLASIVLALVFGYIWPPIQQAIHGV
                     GEWLLGAGLLGAFTFGTLNRLLLPLGLHHVLNSMVWFVFGEFTNAAGQVVTGDLHRFF
                     AGDPTAGVFMTGFFPIMMFALPAAALAMYTTARSANKKAVSG"
BASE COUNT          228 a          439 c          414 g          298 t
ORIGIN      
        1 cgagggtgtc tttcaccgag aacggggaag gggtacgttc gtctcctatc caaagccgac
       61 ctgcacatcc tccagtctca ccggtttttc cgagaacatc cgcgccgcgg ggctcgacgc
      121 gatgacgcac attctcgcct tcgacgagga ggaggcgtcc cgcacgatcc gcgagtcgct
      181 gcagcttccc caacacgacc ccatcgtctt caacatcctg cgcctccgtt acgtggaggg
      241 ggagcctttt tcgctcgaga acgcatgggt tcccaagtgc cgttttccgg aactctcgcg
      301 cgatctgctg gaggggcggt cgctctatac gctgatgcgg gagcacttcg gcatgaagct
      361 ttcgcacgcc tcgcagacca tcgagccggt attctgctcc gacttcgagg ccaagaacct
      421 cgatcttgac tccggaactc cggtgctgct cttcagaaga gtcgcctacg ccgagccgca
      481 aactccggtg gagtactcaa agtgcgtcta caggggaaaa cgattcaagt acgagatttc
      541 cttcggtatt tagggcttca gggggcgcgg cccccaattc aatagaggag ggtgttgtac
      601 atgcttaaag gtttgcagaa actcggtaag gcgttgatgc ttccggtggc ggtgctcccg
      661 gcggcggcgc tgcttctcag gcttggcgcg tcggacgtgc tggacatccc gttcatcatg
      721 caggcgggcg cggcgatctt cgacaacctg gcgctgctct tcgccgtcgg catcgcggtg
      781 ggcgtggcgt tcgacggagg cggcgccgcg gccctcgctg gcgcggtggg ctacttcacg
      841 ctgaccaagg ccgtcgtcac catcaactcg acgatcaaca tgggcgtgct cgcgggcatc
      901 gtctccggca tcgtagcggg aatgctctac aataagtacc ataatatcaa gctccccgac
      961 ttcctcggct tcttcggcgg caagcgcttc gttccgatcg cggcatccct tgccagcatc
     1021 gtccttgcgc tcgtcttcgg ctacatttgg cccccgattc aacaggccat ccacggcgtg
     1081 ggcgagtggc ttcttggggc gggactgctc ggcgcgttca ccttcggcac gctgaaccgg
     1141 ctgcttcttc cgctcggatt gcaccacgtg ctcaacagca tggtctggtt cgtcttcggc
     1201 gagttcacca acgcggccgg acaggtggtc acgggcgacc ttcaccgctt cttcgcgggc
     1261 gatccgacgg caggcgtctt catgacgggc ttcttcccca tcatgatgtt cgctcttcct
     1321 gcggctgctc tggcgatgta caccacggcc cgttcggcga acaagaaagc ggtctcggg
//
LOCUS       DAOSWW010000831         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_86933_length_1379_cov_3.459970, whole genome shotgun sequence.
ACCESSION   DAOSWW010000831 DAOSWW010000000
VERSION     DAOSWW010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86933_length_1379_cov_3.459970"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PK442_13465"
     CDS             <1..1071
                     /locus_tag="PK442_13465"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOO88706.1"
                     /translation="FDTFAPSGHAPRAGELWRSEAHARTLERIAGSNARDFYEGEIAA
                     KILEAARKTGGYFSAEDLGSFVPEWVEPIGVNYKGYDVWEIPPNGQGIVALIALSILR
                     GFEFSSHDDPMTVHRQIEAIKMGFADAHRYVADTRFSGVPTAELLAAPYADARRALLG
                     ERAEARNAGNPLPGGTVYLCAADGEGNMISYIQSNYMGFGSGVVIPGAGIALNNRGHC
                     FSLEKGHPNMLEPGKRPYNTIIPAFLTKEGVPVGPFGVMGGFMQPQGHVQVVMNTVDF
                     HMNPQQALDAPRWRWMGGMNVSVEPGFSPALAQSLQRKGHAMKSELESNSFGRGEIIW
                     RTPEGTLGGATESRTDGCAATW"
BASE COUNT          269 a          419 c          432 g          259 t
ORIGIN      
        1 ttcgacacct tcgccccctc ggggcacgct ccccgcgccg gagagctctg gagatcggag
       61 gcgcacgccc gcacgctgga gcggatcgcg gggagcaatg cgcgcgactt ctacgagggg
      121 gagatcgccg cgaagatcct cgaagccgca cggaaaaccg gtggttactt cagcgccgag
      181 gaccttgggt cttttgttcc tgaatgggtc gagcccatcg gcgtgaacta caaagggtac
      241 gacgtctggg agattccccc caacggacag ggaatcgtcg cccttatcgc gctctcgata
      301 ctgcgcgggt ttgagttctc ctcgcacgac gaccctatga ccgttcaccg gcagatcgaa
      361 gcgatcaaga tgggctttgc cgacgcgcac cgctacgtcg cggacacccg attcagcggc
      421 gttccgaccg cggagcttct ggcggctccg tacgccgatg cgcgccgcgc tcttctgggc
      481 gaacgcgccg aagcgcggaa cgccgggaac ccgcttcccg gcgggacggt ctacctctgc
      541 gccgccgacg gcgagggcaa catgatctcc tacatccaga gcaactacat gggtttcggc
      601 tccggcgtcg tgattcccgg ggccggcatc gcgctgaaca accgcgggca ctgcttctcg
      661 ctcgaaaaag ggcacccgaa catgctcgaa ccgggcaagc ggccgtacaa caccatcatt
      721 cccgctttcc tcacgaagga gggcgttccg gtggggccgt tcggtgtgat gggcggcttc
      781 atgcagccgc aggggcacgt gcaggtcgtc atgaacaccg tcgacttcca catgaacccg
      841 cagcaggcgc tcgacgcccc ccgctggcgg tggatgggcg ggatgaacgt ctccgtcgag
      901 cccggcttct ctcccgccct tgcgcagagc ctccagcgca aagggcacgc gatgaagtcc
      961 gagctggagt ccaactcctt cggcaggggc gagatcatct ggagaacacc cgaaggaacg
     1021 ctcggcggcg cgacggaatc acgcaccgac ggctgcgcgg ccacctggta atctctctcg
     1081 tttcagccga cgcgcgaagc ctctccggag catcgggggg gctttatttt tacgagttgt
     1141 tcggccgaat acgtttgtct cgaaaagaga aacacgtccg ggaacgtaaa ggtccgaaca
     1201 tgtatttttt gtttttggga agtctaaggg gagtaaaata aacttttttg cagtaaaata
     1261 tcgcgttgta gaatcaggtt attttttcgg cgggaaacgt ttggggttct ctaatgatga
     1321 ccgaaagcgt ttcgcagccc ccggaggtga tagggaaccg gtaatgagcg agtgcatgc
//
LOCUS       DAOSWW010000832         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_86936_length_1379_cov_3.335347, whole genome shotgun sequence.
ACCESSION   DAOSWW010000832 DAOSWW010000000
VERSION     DAOSWW010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86936_length_1379_cov_3.335347"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /gene="glmU"
                     /locus_tag="PK442_13470"
     CDS             complement(<1..1244)
                     /gene="glmU"
                     /locus_tag="PK442_13470"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOO88707.1"
                     /translation="MSASRCVTGALVLAAGKGTRMKSDLPKVLLPVLDQPVLYYILDT
                     LRRCMASSKGCVQQLAVVVGHRGETVQEYLGSNWPEALPLWQHEQLGTGHAVQIAKEW
                     WTGLDHLLVLPGDVPLLTEASLSGLVEEHIVSGADCSFISFHASDPSGYGRVVREGKE
                     ISIVEDKDASEAQKALTEVNSGIYIFKTSSLLPHIYRLDNENAQGEYYLPDIIAMMIR
                     AGGKVTASLSSDEGEFAGINTPLQLADAATALRRRILRRHLLAGVRMQDPDSVWIGPE
                     VVIADNVLLEPSVQLWGKTEVERGAHVGSFSILRNVVLEEDACVVSHAVLSDSVFRKG
                     SQVGPFIVVRDGAVFEEDSRGGKFVEVKKSRVGKGSKVPHLAYIGDAEIGAETNIGAG
                     TITCNYDGKKKNPTTIGNRCFIG"
BASE COUNT          285 a          431 c          380 g          283 t
ORIGIN      
        1 cctatgaaac agcggttgcc gatggtcgtg ggattcttct tttttccgtc gtagttgcac
       61 gtgattgtcc cggcgccgat attcgtctcc gcgccgatct ccgcatcgcc gatgtacgcc
      121 aggtggggga ctttgctccc tttgcccacg cggctcttct tgacctcgac gaactttccg
      181 ccccgcgagt cctcttcgaa gaccgctccg tcgcgaacca cgataaacgg accaacctga
      241 gagcctttcc ggaacacgct gtccgagagc accgcatgac tcacgacgca ggcgtcctct
      301 tccaggacga cgttccggag aatcgaaaag ctgccgacat gtgcgcctct ttccacctcc
      361 gtcttccccc acagctggac agacggttca agcagcacgt tgtcggcgat aacgacctcc
      421 ggccctatcc acacggaatc gggatcctgc atacgaaccc cggccaggag atgacggcgg
      481 agaatacgcc tgcgcagggc cgtagccgca tccgcaagct gcaacggagt gttgatcccg
      541 gcaaattccc cttcgtccga ggagagcgag gcggtcacct tgccgcccgc gcggatcatc
      601 atcgcgatga tgtcgggtaa atagtattct ccttgggcgt tctcgttgtc cagtcggtag
      661 atatggggta gaagggagga tgttttgaag atatagatac cgctgttgac ctctgtgagc
      721 gctttttgcg cctccgaggc gtccttgtct tcgacgatcg agatctcttt gccttcccgc
      781 acaactcttc cgtagccgga gggatcggac gcgtggaaac tgatgaagga acagtccgct
      841 cctgaaacga tatgctcctc gacaagaccg gaaaggctgg cctccgtaag aagcggcacg
      901 tctcccggga gaacgaggag atgatcgagg ccggtccacc actccttggc tatctgtacg
      961 gcatgtccgg tcccgagctg ctcatgctgc cacaaaggga gcgcctcggg ccaattcgaa
     1021 ccaagatact cctgaaccgt ctcccctctg tggccgacaa cgaccgcgag ctgctgcaca
     1081 caccccttgg aggatgccat gcaccgccgg agcgtgtcga ggatataata gagcaccggc
     1141 tggtccagaa ccggaagcag gactttcgga agatcgcttt tcatcctggt cccctttccg
     1201 gcggcgagta ccagcgcccc ggtgacacaa cgagaagcgg acacgtgaat caacaccttt
     1261 acattgaatt ttccacaaca caaaaaaagg ggatccgctt caggccccct ctgcccactg
     1321 tacatggctg gggtggctgg attcgaacca gcgatggcgg atccaaagtc cgctgcctt
//
LOCUS       DAOSWW010000833         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_87047_length_1378_cov_3.183673, whole genome shotgun sequence.
ACCESSION   DAOSWW010000833 DAOSWW010000000
VERSION     DAOSWW010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87047_length_1378_cov_3.183673"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PK442_13475"
     CDS             complement(<1..717)
                     /locus_tag="PK442_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88708.1"
                     /translation="MAVTPDLGKFIPVRTEDLVDVLCEEQTLSAEDREKFRRVAELVE
                     ATYHYDFHSTLKEARGAYHIFNPDADTLKICPPGESTDARFDSMVKALEEILVAANYR
                     EFPIAELNELMTKAAPKGLNVRVNLENYEKILIYRRGTKQVPKPKEPLSWWKSLRKKD
                     APVEPEMEEMIQRLLILIKLKSQEDMEQFYDTFIKTAAETENERERRMTREAVLGKKE
                     KETAAKKSNGAPKVPTIFLKV"
     gene            1035..>1378
                     /locus_tag="PK442_13480"
     CDS             1035..>1378
                     /locus_tag="PK442_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88709.1"
                     /translation="MDSLLEYLEFFLKFVLPALSAIAVWYWNERCKRLADQYQRKEAA
                     YRELIRALRGFYVGAENADSLKEEFLNQLNLTWLYCPDDVIKKGYAFLDTVHVRQVRT
                     DEEKEAALGEFV"
BASE COUNT          285 a          346 c          376 g          371 t
ORIGIN      
        1 gaccttcagg aagatcgtag gcaccttcgg cgcgccgttg ctcttcttcg cagcagtttc
       61 cttctccttt ttgccgagca ccgcctcgcg agtcatgcgg cgctcgcgtt cgttctcggt
      121 ctccgcggcg gtcttgatga aggtgtcgta gaactgctcc atgtcctcct gactcttcag
      181 cttgatcagg atgagcagcc gctggatcat ctcctccatc tccggttcaa cgggcgcgtc
      241 cttcttccgg agcgacttcc accaggagag cggttccttc ggctttggaa cctgcttcgt
      301 tccccgccgg tagatcagta tcttctcgta attttcaaga ttgacccgaa cgttcagccc
      361 cttgggggcc gccttcgtca tcagttcgtt cagctcggcg atgggaaact cgcggtagtt
      421 cgccgctaca aggatttctt cgagcgcctt caccatgctg tcgaagcggg cgtccgtgct
      481 ttcgccgggc ggacagatct tcagggtgtc ggcgtcgggg ttgaagatat ggtaggcgcc
      541 gcgcgcctct ttcagcgtac tgtgaaagtc gtagtggtac gtcgcctcga ccagttcggc
      601 gacgcggcgg aacttctccc tgtcctccgc cgaaagggtc tgctcctcgc acagaacgtc
      661 cacaaggtcc tcggtgcgca cggggatgaa cttgcccaga tccggagtaa ccgccatcgc
      721 aacccctcct gtattaaaaa gttcgacgcg tcctctgcgg ggaagagccc gcccgaagaa
      781 tccgtcgtcg gaaacaaacc gtctccacat agtacattca tccggcctgt ttatcaatag
      841 tgtgaaaagc ttgccgaatt gtgcggggtg tggtagaaat ggggagaaaa aaggattgac
      901 ggacgaacag ccgctatccg ctcatttgcg aatgagtagt ttgtaggatg ttctctgctt
      961 aatggaatga ggggtttaag aattttctcc tttttttgtt gaatcatcta tctcatatta
     1021 gggggcgttg atctgtggat tctcttcttg agtatctgga gtttttttta aagtttgtgc
     1081 ttccagcttt aagcgctata gctgtttggt attggaatga acggtgtaaa cgccttgccg
     1141 atcagtatca acggaaggaa gcagcatatc gagagttgat ccgagcattg agaggttttt
     1201 atgtgggtgc agagaatgca gattctctca aagaagagtt tcttaaccaa ctcaacttaa
     1261 cgtggcttta ctgtccggat gacgtcatca aaaaaggtta tgcgtttttg gacacggtgc
     1321 atgttcgtca agtgagaacg gatgaagaga aagaagctgc attaggcgaa tttgtata
//
LOCUS       DAOSWW010000834         1377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_87176_length_1377_cov_2.621785, whole genome shotgun sequence.
ACCESSION   DAOSWW010000834 DAOSWW010000000
VERSION     DAOSWW010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87176_length_1377_cov_2.621785"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1377)
                     /locus_tag="PK442_13485"
     CDS             complement(<1..>1377)
                     /locus_tag="PK442_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HOO88710.1"
                     /translation="GLAAGVASICYRLLLGYAEEFSLRALRGGYSLSLPVLLLLWTAV
                     ALFIGKLMEKEPMARGSGIPQVEGELMGCVSMNWLRVLWAKFLGGGLAIALGLSLGRE
                     GPSVQIGAAVGKGLSRLQKKTKMEEKLLLSSGASAGLAAAFNAPLSGVVFALEEVHKS
                     FSPLILLSAMMASLGADFLSKTFFGLGPVFSFGVPAPLPLGFYGHFLVLGVLCGVGGA
                     LFCRAILELQTLYAKLPIPASFRVVIPALVAVVAGSFLPEILGGGHALVEYLGHGSHP
                     ASFILLLFAGKLLFTALCFGSGAAGGIFLPMLAVGASLGAFYGTVAVQVYGLDQALVL
                     NFLIVGMAGFFSAVVRAPITGIVLITEMTGSFTHLLSVSAVAVTAYVVADLLRARPIY
                     DSLLLRMLRKCDTCEEAGKVLLEGTVSSGSSLDGKRVRDARLPAGCLLVSIDRAGEEI
                     LPNGETVLA"
BASE COUNT          328 a          459 c          415 g          175 t
ORIGIN      
        1 gcgagcacgg tctctccgtt cggaagaatc tcctcccccg cccggtcgat gctcacaagc
       61 aggcaccccg ccggaagccg tgcatcccgc acgcgcttcc cgtcaagaga cgatcctgaa
      121 gaaaccgtgc cttcgagcag cacctttccc gcctcctcgc acgtatcgca cttgcgcagc
      181 atgcgcagaa gcagcgaatc atagatcggc ctcgcgcgca gcaggtccgc gacgacgtag
      241 gccgtcaccg ccaccgcgga gacggagagc agatgggtaa acgatccggt catttcggtg
      301 atgaggacaa tccctgtgat cggggcgcgg actacagccg agaaaaaacc ggccatgccg
      361 acgatgagaa agttgagcac cagcgcctga tcgaggccgt agacctggac ggcgacggtc
      421 ccgtagaatg cgcccagcga cgcgccgaca gcgagcatgg ggagaaagat gcctcccgcc
      481 gctcccgagc cgaaacagag cgcggtgaag aggagcttcc ccgcgaagag caacaaaatg
      541 aagctcgcag gatgactccc gtggccgaga tactccacga gcgcgtgccc tcctccgaga
      601 atttcgggga gaaaagaacc cgcaacgacg gcaacgagcg cggggatgac gacgcgaaag
      661 gacgcaggga tcggaagttt cgcgtagagc gtctgcagtt ccagaatagc gcggcagaag
      721 agcgcgcccc cgacgccgca gagcaccccg agcaccagaa agtgcccgta gaacccgagc
      781 ggcagggggg cgggaacgcc gaaagagaac accggtccca gcccgaaaaa tgtcttcgaa
      841 agaaagtccg ccccgagcga agccatcatc gccgagagca aaatcagggg ggagaagctc
      901 ttatgtacct cttccagggc aaagaccacc cccgagagcg gcgcgttgaa cgcggcggcg
      961 agccccgccg acgcgccgct ggagagcagc agcttctcct ccatcttcgt ctttttctga
     1021 aggcgggaga gccctttccc gaccgcagcg ccgatctgca ccgacggccc ttcacgtcca
     1081 agcgagagcc cgagggcgat cgccagaccg ccgccgagaa acttcgccca cagcactcgc
     1141 agccagttca tcgagacgca ccccataagt tcgccctcga cctgaggaat tccgcttccc
     1201 cgcgccatcg gctctttttc catgagcttg ccgatgaaga gcgcgacagc tgtccacagg
     1261 agcagaagca cgggaaggga aagcgaatag ccccccctca acgcacgaag agagaactct
     1321 tccgcatacc ccaggagcag gcggtagcag atagaggcga cccccgcagc gagcccg
//
LOCUS       DAOSWW010000835         1375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_87379_length_1375_cov_2.884848, whole genome shotgun sequence.
ACCESSION   DAOSWW010000835 DAOSWW010000000
VERSION     DAOSWW010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87379_length_1375_cov_2.884848"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PK442_13490"
     CDS             <1..576
                     /locus_tag="PK442_13490"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88711.1"
                     /translation="EVIERAMPHCPLEEFRPFVVDPEERAITPLPPFGGDYVIRITGS
                     MHDERGGQNATPENADRVIRHLTDKLEKNRADIIRVREYALDDAEYALVSFGCSARAS
                     RAAVKQARALGKRVGLLELTTLWPFPDDEVRSLLSRVKGAAVVEMNLGQVVREVRRLN
                     DFGIPVLGVHRVDGMLVAPADILSRTEEAFS"
     gene            573..>1375
                     /locus_tag="PK442_13495"
     CDS             573..>1375
                     /locus_tag="PK442_13495"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO88712.1"
                     /translation="MSMLTYLDYLAAHKLPTIWCPGCGNGIVLKAILKAFADLGLDKR
                     KTVAVSGIGCWGWADDYIDVNAFHGTHGRALAFATGIKLANPELTVIVLLGDGDGATI
                     GGNHLIHAARRNVDLTAVVVNNFNYGMTGGQYSGTTPAGSKTSTSPLGHAEDPFDLCA
                     LAAAAGAPYVARGSVRDGAALRRLVRNGLSRRGFSFIEALSICPTHYGRRNDARTPFE
                     MYQRLGDMTLPLEKSALLSEEERHGKLLLGTFADRAPDDWSARYKQATKR"
BASE COUNT          229 a          478 c          467 g          201 t
ORIGIN      
        1 gaggtcatcg aacgtgcgat gcctcactgc cccctcgaag agttccgccc gttcgtcgtc
       61 gacccggagg agcgcgcgat cacgcccctt ccccccttcg gcggcgacta cgtcatccgc
      121 atcaccggct cgatgcacga cgagcggggc ggacagaacg ccaccccgga gaatgcggac
      181 cgagtgatcc ggcacctgac ggacaagctg gagaagaacc gcgccgacat catccgggtg
      241 cgcgaatacg ccctcgacga cgcggagtac gcgctcgtct ccttcggctg ttccgcccgc
      301 gcctcgcgcg cggcggtgaa gcaggcccgc gccctcggga agcgcgtcgg cctgctggag
      361 ctgaccaccc tctggccctt ccccgacgac gaggtgcgtt cgctgctctc ccgcgtgaag
      421 ggcgccgccg tggtggagat gaatctcgga caggtcgtgc gcgaggtgcg gcggctgaac
      481 gacttcggca tccccgtcct cggcgtccac cgggtcgacg ggatgctcgt cgcgcccgcc
      541 gacattctct cccgcacgga ggaggcgttc tcatgagcat gctcacgtat ctggactacc
      601 tcgcggcgca caagctcccc accatctggt gccccggctg cggcaacggc atcgtgctca
      661 aggccattct gaaggccttc gccgacctcg ggctcgacaa gcggaagacc gtggccgtct
      721 ccggaatcgg ctgctggggg tgggcggacg actacatcga cgtgaacgcc ttccacggca
      781 cgcacggacg ggcgctggcc ttcgccacgg ggatcaagct ggcgaacccc gagctgaccg
      841 tcatcgtgct cttgggggac ggcgacggcg cgaccatcgg cggcaaccac ctgatccacg
      901 cggcgcgccg gaacgtcgac ctcaccgcgg tggtggtgaa caacttcaac tacggcatga
      961 cgggggggca gtactccggc acgacaccgg cggggagcaa aacctccacg tcgccgctgg
     1021 ggcacgcgga ggacccgttc gatctctgcg cgctcgccgc cgctgcggga gccccctacg
     1081 tggcgcgggg ctcggtgcgc gacggggcgg cgctgcggcg gctcgtccgg aacgggctct
     1141 cgcggagggg attctcgttc atcgaggcgc tctccatctg ccccacgcac tacgggcgac
     1201 ggaacgacgc gaggacgccg ttcgagatgt atcaacggct gggcgacatg acgctcccgc
     1261 tggaaaagag cgcgctgctg agcgaggaag agcggcatgg aaagctcctt ctgggcacgt
     1321 tcgcggatag agcgcccgac gactggagcg ctcgatataa gcaggcgacc aaacg
//
LOCUS       DAOSWW010000836         1371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_87790_length_1371_cov_2.964286, whole genome shotgun sequence.
ACCESSION   DAOSWW010000836 DAOSWW010000000
VERSION     DAOSWW010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87790_length_1371_cov_2.964286"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PK442_13500"
     CDS             <1..873
                     /locus_tag="PK442_13500"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOO88713.1"
                     /translation="TGSQHFHLLENITQSLAGRTALLQLPSFSFDELRSAGLLPESAD
                     EAILSGGYPPLHTRTVERTAWFANYTATYLERDVRSMLNVRDLSTFQRFLRLCSGRAG
                     QLVNFSSLAGECGVVHNTAKAWLSILEAGSIVFLVRPYFRNFNKRLLQTPKLYFHDTG
                     LLCHLLGIRSEEQLETHPLRGAVFENMVAVELLKARLNEGREPELYFWRDRSGFEIDF
                     LIENDGLLDAIEVKSGKTVTREFFRAIGRWKEIAGESAGKTFLIYGGDDEYEREGHSV
                     LSWRNAGGIAGKCR"
     gene            1044..1316
                     /locus_tag="PK442_13505"
     CDS             1044..1316
                     /locus_tag="PK442_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88714.1"
                     /translation="MKMVSVREIKKNPAVLYSGPGYRVLTSNNRPKALCFPLDELDDI
                     EEIVASFRQAQAMRAVERVRSFAVEKGLDGMTMEDVDEEIRRARNG"
BASE COUNT          291 a          402 c          404 g          274 t
ORIGIN      
        1 accggctcgc agcactttca ccttttagag aacatcacac agagccttgc cggcaggacg
       61 gcgctcctgc agctcccttc cttctctttc gacgaactgc gaagcgccgg gttgctcccc
      121 gaaagcgccg acgaagcgat cttgtccggc ggctatcctc cgctgcacac ccgaacagtg
      181 gagagaaccg catggttcgc caactatacc gcgacctacc ttgagcgcga tgtacgctcg
      241 atgctcaacg tccgcgacct ctccactttt cagcgcttcc ttcgcctgtg ctccgggagg
      301 gccgggcagc tcgtcaactt ctcttctctg gcgggggagt gcggcgtcgt ccacaacacc
      361 gccaaggcgt ggctttccat cctggaggca ggctccatcg tctttcttgt tcgcccatac
      421 ttccgcaact tcaataagcg cctcctccag accccgaaac tctacttcca cgacaccggg
      481 ctgctctgtc accttctcgg catccggagc gaagagcagc tcgaaacgca ccccctgcga
      541 ggcgccgtct tcgagaacat ggtcgccgtc gagcttttga aggcgcgcct caacgaaggg
      601 cgcgaacccg aactctattt ctggcgcgac cgaagcggct tcgagatcga cttcctgatc
      661 gaaaacgacg gtcttctgga cgcgatcgag gtaaaatccg gaaagacagt gacccgcgag
      721 ttcttccgag cgatcggacg ctggaaggag atcgccggcg agagcgccgg aaagaccttc
      781 ctgatctacg gcggagacga tgagtacgaa agggaagggc attcggtcct ctcgtggaga
      841 aacgccggcg gcatcgcggg gaagtgccga tgacagtcgc gcatgctcag cgacgatccg
      901 caggagcgcg agctgcttga tttcatcgaa accgcaacgg atacggagag ttgggagtga
      961 aaacgagata cgccccctgt ttcgagatac aatagcggtg ctgatgtagc atatatcgaa
     1021 ggattaaacg gaggtggttg atcgtgaaga tggtgtccgt tcgggagatc aaaaagaatc
     1081 cggcggttct ttacagcggc cccggatacc gtgtgctgac atccaataat cgcccgaagg
     1141 cgctctgttt ccccttggat gagttggacg acatcgagga gatcgtggcg agctttcggc
     1201 aagcccaagc gatgagggcg gtggaacgag tacgctcctt cgctgtcgag aagggactcg
     1261 acggaatgac tatggaagat gtcgacgagg agatacgtcg cgcccgtaac ggttagtgta
     1321 gcggcttgat ctccttgtcc ggggtcgact ctttttcgat gaccgcggag a
//
LOCUS       DAOSWW010000837         1370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_87873_length_1370_cov_4.673764, whole genome shotgun sequence.
ACCESSION   DAOSWW010000837 DAOSWW010000000
VERSION     DAOSWW010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87873_length_1370_cov_4.673764"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PK442_13510"
     CDS             <1..711
                     /locus_tag="PK442_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOO88715.1"
                     /translation="TIFLEDEASAMREEDMKEALRRIGKTRPEDEVNCGGCGYASCRE
                     LAAALALGRAEPEMCVIHMRNQAQKKANALLRTMPSGVVIVDKSLHIVECNERFARLF
                     GESAMLVYRAEPGLKGCILGRILPFTHLFELVLEKGQDIHLDHYRTGDTLLDITLFPI
                     EPGETVGAVMLDVTHKELKRDKIAQRAAEVIERNILTVQEIACRLGEHMADTEMLLRS
                     IAEGYSESDEDVSQEDAR"
     gene            708..>1370
                     /locus_tag="PK442_13515"
     CDS             708..>1370
                     /locus_tag="PK442_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO88716.1"
                     /translation="MKQEEPLFLDVDFDQRKKAGQYVCGDSFFSRKLPGRNRVVSVLS
                     DGLGSGVKASILSSMTGTMALRFMEGDMEILRSAEVMMDALPVCRKRGISYATFTIVD
                     AVLHGWTRVIEMDNPSFMLVRNGRLLAPGKREMASSRWKDRKITVNEFFARPEDRIIL
                     FSDGVTQAGMGSWANPTGWGQEKCAAFILDTLRERPGISSRELSRRVVSEALVREPHE
                     RAG"
BASE COUNT          260 a          423 c          465 g          222 t
ORIGIN      
        1 actatctttc ttgaggatga ggcgtccgcg atgcgggagg aagacatgaa agaagccctt
       61 cgccgcatcg ggaagacgcg ccctgaggac gaggtgaact gcggggggtg cggctacgcc
      121 tcctgccggg agctcgcggc cgcgctggcg cttggtcggg cggagcctga gatgtgcgtc
      181 atccacatgc ggaaccaggc gcagaagaag gcgaacgcgc tgctccggac catgccctcc
      241 ggcgtcgtga tcgtggacaa aagcctgcac atcgtcgagt gcaacgagcg cttcgcccgt
      301 ctcttcggcg agagcgcgat gctcgtctac cgcgccgagc cggggctgaa agggtgcatc
      361 ctcggcagaa tcctgccctt cacccacctc ttcgaactcg tgctcgaaaa ggggcaggat
      421 atccacctcg accactaccg caccggcgac acgctgctgg acatcacgct cttccccata
      481 gaaccgggag aaacagtcgg cgccgtgatg ctcgacgtca cccacaaaga gctgaagcgc
      541 gacaagatcg cccagcgcgc cgcggaggtc atcgaacgga acattctcac ggttcaggag
      601 atcgcgtgca gactggggga gcacatggcg gacacggaga tgctgcttcg ttcaatcgcc
      661 gaggggtatt ccgaatcgga cgaggacgtt tcgcaggagg acgcgcgatg aaacaggaag
      721 agcctctttt tctcgacgtc gatttcgacc agcgaaagaa ggccgggcag tacgtctgcg
      781 gcgacagctt cttctcccgc aagctgccgg ggcggaacag ggtggtttcg gtgctctccg
      841 acggtctcgg cagcggagtg aaggccagca ttctctcgtc gatgacgggg acgatggcgc
      901 tccgtttcat ggagggggac atggagatcc tccgctcggc ggaggtgatg atggacgcgc
      961 tccccgtctg ccggaagcgc ggcatcagct acgccacctt cacgatcgtc gacgcggtgc
     1021 tccacggctg gacgagggtg atcgagatgg acaacccctc cttcatgctg gtgcggaacg
     1081 gacggctcct cgcccccgga aagcgggaga tggcctcctc ccgctggaag gaccggaaga
     1141 tcacggtgaa cgagttcttc gcccgcccgg aagaccggat cattctcttc tccgacgggg
     1201 tgacgcaggc cggaatggga tcgtgggcca acccgacggg gtgggggcag gagaagtgcg
     1261 ccgccttcat cctcgacacg ctccgggagc gcccgggcat ctcctcgcgg gagctgtcgc
     1321 gccgggtcgt ctccgaagcg ctcgtcaggg agccgcacga gcgggccggg
//
LOCUS       DAOSWW010000838         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_87980_length_1369_cov_4.506088, whole genome shotgun sequence.
ACCESSION   DAOSWW010000838 DAOSWW010000000
VERSION     DAOSWW010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87980_length_1369_cov_4.506088"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PK442_13520"
     CDS             complement(<1..208)
                     /locus_tag="PK442_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO88717.1"
                     /translation="MAIKLSTKTRYGLRALLYMAERYDGERTVPVGEIAKEQEVSEKY
                     LEQLFLKLRRSNLIQSVRGAQGGYM"
     gene            complement(339..1109)
                     /locus_tag="PK442_13525"
     CDS             complement(339..1109)
                     /locus_tag="PK442_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO88718.1"
                     /translation="MSDAVMNEKKALLEVHDLHVSYGAIKALSGVSLKVFEGEIVSVI
                     GSNGAGKSTLMNAIMGMVRREKGEILLGDVKLDSRSFRVVQQGISLSPEGRKVFAPLT
                     VLENLKMGAFPRGNDSKVEEDYEWVFSLFPRLKERISQYAGTLSGGEQQMLAIGRALM
                     SRPKVLLLDEPSLGLAPIIIKDIFKELRRINNEGVTILLVEQNARQALLLSNRGYVIQ
                     TGRLVTEGPSEELLANADIKAAYLGGGHAKGKTTQALA"
     gene            complement(1106..>1369)
                     /gene="livG"
                     /locus_tag="PK442_13530"
     CDS             complement(1106..>1369)
                     /gene="livG"
                     /locus_tag="PK442_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047571.1"
                     /note="Part of the ABC transporter complexes LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HOO88719.1"
                     /translation="PKLLLLDEPAAGMNAEEVLSLNSLIRSIHTDFKLTILVIEHHMD
                     LIMEICPHIVCMNFGAKIAEGTPEEIQNHPEVLKAYLGEEEEE"
BASE COUNT          300 a          382 c          350 g          337 t
ORIGIN      
        1 gcatatagcc gccctgcgcc ccccggacgc tctgtataag gttgctgcgt ctaagcttga
       61 gaaagagctg ttcgaggtat ttttcggaaa cttcctgctc cttggcgatc tcgccgacgg
      121 gcacagttcg ttcaccgtcg tatctttcag ccatatagag caaggcgcga agaccatagc
      181 gagtttttgt cgagagttta attgccatca cttcccctgc tctgaactga cgacagagta
      241 ctatatccta ctatttttgt caagtttatt atcgaaaaaa acacccggtc ttccggagag
      301 accgggtgtt tctcgtaaat tcagcgagag agcaaggatc aggcaagcgc ttgggtcgtc
      361 ttccccttcg cgtggccccc gccgagatag gccgccttga tgtccgcatt cgcaagaagt
      421 tcctccgagg gaccttccgt gaccagacgc ccggtctgaa tcacatagcc gcgatttgag
      481 agcagaagag cctggcgggc gttctgttca accagcagga ttgtgactcc ttcgttatta
      541 atcctgcgca gctctttgaa gatatccttg atgataatgg gggcaagtcc gagcgaaggc
      601 tcgtcaagga ggagcacctt cggacgggac atgagcgccc tgccgatggc gagcatctgc
      661 tgttctcctc ccgaaagcgt gcccgcgtat tggctaatcc gctccttcag acggggaaaa
      721 agggagaaga cccattcata gtcttcttcg actttactgt catttccccg agggaaagct
      781 cccatcttca agttttccag aacagtgaga ggggcgaaga ccttgcggcc ctcggggctg
      841 agcgaaatcc cctgctgtac caccctgaaa cttctcgagt caagctttac gtcgccaaga
      901 aggatttctc ctttttctcg ccgcaccata cccatgatag cattcatcag cgtagatttg
      961 cccgcgccgt tcgaaccgat gacgctgacg atctcgcctt cgaacacctt cagagagact
     1021 ccggagagag ccttgatggc gccgtagctc acgtggaggt catggacttc gaggagggct
     1081 tttttctcgt tcataaccgc gtcgctcatt cctcttcctc ctctccgaga tacgccttga
     1141 gaacttccgg atggttctgg atttcctcgg gcgttccctc ggcaatcttc gcgccgaagt
     1201 tcatacatac gatatgagga cagatttcca tgatgaggtc catgtgatgc tcaataacga
     1261 gaatcgtcag tttgaaatca gtgtgaatgg accggatcaa cgaattcagc gagagaacct
     1321 cttccgcgtt cattcctgca gcaggctcgt caagcagcag cagcttcgg
//
LOCUS       DAOSWW010000839         1366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_88343_length_1366_cov_3.548436, whole genome shotgun sequence.
ACCESSION   DAOSWW010000839 DAOSWW010000000
VERSION     DAOSWW010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88343_length_1366_cov_3.548436"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PK442_13535"
     CDS             complement(<1..1189)
                     /locus_tag="PK442_13535"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOO88720.1"
                     /translation="MLVSGDRFLADLEALGRIGWVEGEGMRRPAYSPSYDEARRFVAA
                     KMKEAGLTVRVDGVGNLFGRLEGSDPSLPSIMSGSHLDAVPGGGKYDGPLGVMAALEA
                     ARSIKERKLPLRHPFEAVGFTAEEGGEMGGTFGSRAMAGLLDEPLPEEKLASVGLTPE
                     MVRSSKRDPSRIACYIELHIEQGPFLERRGISIGIPTGIVGIGRYAVRLTGEANHAGT
                     TPMKERRDAMREAAELLSEWFAWTDARDDMVCNVGVFSIHPGAAAVVPDRAEFTLEIR
                     SLKDSVMEEAASAFRRLLEKRTRVAVSMEPLIWKGAVELSPLLQEAAEKACGEMGVSC
                     VRMPSGASHDAAPMAGIVPAGMLFVPSAGGISHAKEEATPPDDLVRGAEALLRTVLEA
                     DARL"
BASE COUNT          228 a          492 c          422 g          224 t
ORIGIN      
        1 acaggcgcgc gtcagcttca agcacagtcc tcagaagggc ctccgcccct cgaacaagat
       61 cgtccggcgg ggtcgcttct tctttggcat ggctgatacc gcccgcgctg ggaacgaaga
      121 gcatcccggc cgggacgatg ccggccatcg gggctgcgtc gtgcgacgcg ccgctcggca
      181 tcctgacgca ggagacgccc atctcgccgc atgccttttc ggccgcctcc tggagcagcg
      241 gggagagttc caccgctcct ttccaaataa gcggttccat cgacacggcg acgcgggtcc
      301 gcttctccag aagcctgcgg aacgcagagg cggcctcctc catcaccgag tccttcagcg
      361 agcggatctc aagcgtgaac tccgcccggt cgggcacgac cgccgcagcc ccgggatgaa
      421 tcgagaagac gccgacgttg cagaccatgt cgtcccgcgc gtccgtccac gcgaaccatt
      481 cgctcagcag ctccgcagcc tcgcgcatgg cgtccctgcg ctccttcatc ggcgtggtcc
      541 cggcgtggtt cgcctcgccg gtcaggcgga cggcgtaccg gccgatgccg acgatgccgg
      601 tagggatgcc gatggatatc ccccgccgct caaggaaggg gccctgctcg atgtggagct
      661 caatgtagca ggcgatccgc gaaggatccc tcttcgacga gcgcaccatt tccggggtga
      721 gccctacgga ggcaagtttt tcctccggga gcggctcgtc gagcagccct gccatcgcgc
      781 ggcttccgaa ggtaccgccc atctcgcctc cctcctcggc ggtgaagccc actgcctcga
      841 aggggtgacg gagcggaagc ttccgttctt tgatggagcg cgccgcctcc agcgcggcca
      901 tgacgccgag agggccgtcg tacttcccgc ctccgggaac cgcgtcgaga tgggaaccgg
      961 acatgatgga gggaagcgac gggtcgctcc cctccagcct gccgaagagg tttcccacgc
     1021 cgtccactct gacggtcaac ccagcctctt tcatcttcgc cgcaacaaaa cgccgcgcct
     1081 cgtcatagga gggggaatat gccggacggc gcattccctc cccctcgacc cagccaatgc
     1141 gcccaagcgc ctcaagatcg gcgagaaaac gatccccgga gacgagcatg gctacaccgc
     1201 cggaacgccc agctccttca gtatccgggc gatctccttc ttcgtctcct cgctcgcggg
     1261 gaggatcggc aggcgcggga ggcctccctt gtagccgacc atgtccatcg cggccttcat
     1321 gccgccgatg ccgaaccggc tggtgaccgc ggcgttcagc ggcagc
//
LOCUS       DAOSWW010000840         1366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_88345_length_1366_cov_3.519451, whole genome shotgun sequence.
ACCESSION   DAOSWW010000840 DAOSWW010000000
VERSION     DAOSWW010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88345_length_1366_cov_3.519451"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PK442_13540"
     CDS             complement(<1..678)
                     /locus_tag="PK442_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOO88721.1"
                     /translation="MVSAIHNNLQRVREKLADAVRKSGRNDEDVRLVAVTKTYPVEDI
                     LEAVRCGVVSIGENRVQELSEKRSLWSGPSIEWRFIGHLQGNKVRKVLEFASTVDSLD
                     SLSLARALSRVAEEKKMRLPVLIEVNTSGEESKHGTAPSDAAPLLESVMKECPALQVE
                     GLMTVGPLTDDEAEVRRAFALLRGIRDSLSGAFGLRLKELSMGMSGDYEWAVDEGSTM
                     VRIGSAIF"
     gene            complement(672..>1366)
                     /locus_tag="PK442_13545"
     CDS             complement(672..>1366)
                     /locus_tag="PK442_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88722.1"
                     /translation="EPGTRRVARGETVAVIGSSSGKMAVRSESVGYFVPGLDGAEGEW
                     RYSFHWPGMAPLPASPAFAEFSSGVFVRRGDPVGKLISQPQELRCVLYADVTPALQKD
                     IQTGFVRVKTEAHGWPGRAAVRASRFLGTKMKLYLTLPFFSLENVKSREIALFLETGE
                     HVGVAVPESSVRYREGKLGVFQVEGNIVKFKEIEGLPVEGHRFFVQKGLQSGNIVVLN
                     AERAKEGKIRLW"
BASE COUNT          322 a          444 c          307 g          293 t
ORIGIN      
        1 gaagatagcg gagccgatac ggaccatcgt acttccctca tcaacagccc attcgtaatc
       61 tccgctcatc cccatggaaa gctctttcaa gcggagaccg aacgctccgc tcaaagaatc
      121 cctgatcccc cgcagcagcg cgaaggctct tcgtacctcc gcctcgtcat cggtcaaagg
      181 tcccacagtc atcaggcctt ctacctgaag cgcgggacat tccttcatca cgctctcaag
      241 aagcggcgcc gcgtcggaag gagcagtacc atgtttgctc tcctctccgg aagtattcac
      301 ctcaatgagc accggaagac gcattttttt ctcttccgca acacgcgaga gagccctggc
      361 gagcgacaat gaatccaggg aatcgaccgt ggatgcaaat tcaagaacct tcctcacttt
      421 gtttccttgc agatgcccga taaaacgcca ctctatggaa ggaccgctcc aaagacttcg
      481 cttctccgag agttcctgta cccggttctc ccctatcgag acgacgccgc accggacggc
      541 ttcaagaata tcctccaccg gataggtttt tgtcaccgcg acaagccgaa cgtcttcgtc
      601 attccttccg cttttgcgga cagcatcggc aagtttttcc cgcacacgtt gaaggttatt
      661 gtgtattgca ctcaccatag tcggattttc ccttccttcg ctctttctgc attgaggacg
      721 acgatattgc ccgactgcaa ccccttttgg acaaaaaaac gatgtccttc cacgggcaga
      781 ccttcgatct ccttgaattt tacgatattc ccctccacct ggaacacccc gagctttccc
      841 tcgcgatatc gaacagagga ttccggcacg gcaacaccga catgctcccc cgtctcgaga
      901 aacagcgcaa tctccctgga tttgacattc tcgagagaaa agaacggcaa tgtaagatag
      961 agcttcattt tcgtacccaa aaaacgcgac gcacggacgg cagcccttcc cggccacccg
     1021 tgcgcctcgg tcttaacacg cacaaaacct gtttgtatat ctttttggag cgcgggcgtc
     1081 acatcggcgt agaggacgca ccgaagctcc tgaggctgag atatgagctt tcccacggga
     1141 tctccccggc gaacaaacac cccggatgaa aattcggcaa aagccggaga cgccggcagc
     1201 ggcgccatac cgggccagtg gaacgaatac cgccactctc cttcagcccc gtccaggccg
     1261 ggaacaaaat atccgacaga ctctgaacga acagccattt tccccgagga tgaaccgata
     1321 accgcaacag tttcgcctct cgcgactctt ctcgttcccg gctccg
//
LOCUS       DAOSWW010000841         1366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_88406_length_1366_cov_2.257056, whole genome shotgun sequence.
ACCESSION   DAOSWW010000841 DAOSWW010000000
VERSION     DAOSWW010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88406_length_1366_cov_2.257056"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..530
                     /locus_tag="PK442_13550"
     CDS             165..530
                     /locus_tag="PK442_13550"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOO88723.1"
                     /translation="MTLLLKRVYEAPNPEDGFRILIDRLWPRGITKEKAAVDLWLKEA
                     APSPELRKAFCHDPEKWDDFRARYVAELETNAAVDTIMERLREGTVTLVYAAKDETYN
                     HAAALAEHLERQIAVRNGA"
     gene            527..>1366
                     /locus_tag="PK442_13555"
     CDS             527..>1366
                     /locus_tag="PK442_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HOO88724.1"
                     /translation="MSGGGKRGTGRRAKLPGLPTEKQLVAEERKQTTSGIEIMHYAPD
                     GFEEIRNATLEDCARLKDCPGITWINVDDIRDTGAVRSLAALFGLHPLTTEDIGNPAQ
                     RPKAENFEQYLFFALKMLTYDDATHSILNENVSVVLGDRWVVSFQEREGDVLDPVRDR
                     IRSGKGRLRSEGADYLAYAIMDSVVDEYFVVLEKLGDHIEEIDEQILAVPDRTHMKEL
                     HRLKREILFLRKAVWPLREEISTIEKSGSRLVGDSIKPFLRDLYDHTIEIIDMVETNR
                     DIVS"
BASE COUNT          310 a          407 c          428 g          221 t
ORIGIN      
        1 tccggacacg ctttcttgct gcataattat aataatcgca cgggggcacc ttttgcaatg
       61 agtcgcaaga cgcaaatact cacatctcaa tgaaagcagc gcatccgcct tcgaggaaac
      121 ccggtgctat aatcgtttca tgagacctgc ggggagggaa gagcatgacg ctgctgctga
      181 aacgggtgta cgaagcgcca aatccggagg acggcttccg gattcttatc gaccggctat
      241 ggccgcgcgg catcacaaag gagaaggccg ccgtcgacct gtggctgaag gaggccgcgc
      301 ccagcccgga gttgcggaaa gcgttctgcc acgaccccga aaagtgggac gatttccggg
      361 cgcgctacgt cgccgagctg gagacgaatg cggcggtcga tacgatcatg gagcgcctgc
      421 gcgaggggac ggtcacgctc gtctacgccg cgaaggacga gacatacaac cacgccgccg
      481 cgcttgcgga gcatctggag cggcaaatcg cagtgaggaa cggcgcatga gcggaggcgg
      541 aaagcgcgga accggacgcc gagcgaagct gcccggcctt ccaacagaga aacagctcgt
      601 cgccgaagag cggaaacaaa cgacttccgg catcgagatc atgcactacg ctccggacgg
      661 tttcgaggag atccgcaacg cgacgctgga agactgcgcc cggctgaaag actgccccgg
      721 aataacatgg atcaacgtcg acgacattcg cgacacgggg gcagtccgga gcctggcggc
      781 ccttttcgga ctgcacccgc tgacgacgga ggatatcggc aacccggcgc agcgtcccaa
      841 ggcggagaac ttcgagcagt atctcttctt cgccctcaag atgctgacct acgacgacgc
      901 gacgcactcg atcctcaacg agaacgtcag cgtcgtgctg ggcgaccgat gggtcgtctc
      961 attccaggag cgcgaggggg acgttctcga tcccgtcagg gatcgcatcc ggtcggggaa
     1021 gggacgcctc cggagcgaag gcgccgacta cctggcctac gccatcatgg attcggtggt
     1081 ggacgagtat ttcgtcgtcc ttgaaaagct cggcgaccac atcgaggaga tcgacgaaca
     1141 gatcctcgcc gttccggaca ggacgcacat gaaggagctc catcgcctga agcgtgagat
     1201 tctcttcctg cggaaggcag tctggccgct gcgggaggag atttcgacta tcgagaagag
     1261 cggctcgcgc ctcgtcggcg actccataaa gcccttcctt cgcgacctgt acgaccatac
     1321 catcgagatc atcgacatgg tggagaccaa ccgcgacatc gtgagc
//
LOCUS       DAOSWW010000842         1365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_88486_length_1365_cov_2.907634, whole genome shotgun sequence.
ACCESSION   DAOSWW010000842 DAOSWW010000000
VERSION     DAOSWW010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88486_length_1365_cov_2.907634"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK442_13560"
     CDS             complement(<1..594)
                     /locus_tag="PK442_13560"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOO88725.1"
                     /translation="MKILALNPGSTSTKVALFEDHTELWSETQRYDTDVIGRFSGVME
                     QEEFRLEEIRKCLAAHGATVADLDAVVGRGGLLKPIESGTYEVSDKMLEDLRACTWGA
                     HASNLGAPLAVRLADEGGCGKAYIVDPVVVDEMCPLARYSGLPEIERRSIFHALNQKA
                     VARRAAAELQKPYAECNLVVAHMGGGVSVGAHEKGRVI"
     gene            complement(608..>1365)
                     /locus_tag="PK442_13565"
     CDS             complement(608..>1365)
                     /locus_tag="PK442_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOO88726.1"
                     /translation="KMKAAAEQAGVDLANYEVIDERDEPSMGISAVSQVSSKKADIYM
                     KGQIHTNNFLRAMLNKEVGLRKGKNTISHCYFHSVEGYDRIFFIADAAFNMYPDLAAK
                     ADITQNTVGLARSFGVAEPKVAVLAAVEVVNPDMPATLDAAALTQMNRRGQIKNCIVD
                     GPFALDNAVSEESAKTKGIVSPVAGKADIFIVPNIDAGNMLAKGIVYFSKNETAGLIL
                     GASAPIVLTSRADSTRAKLLSIAAAVTLSDFNK"
BASE COUNT          216 a          411 c          457 g          281 t
ORIGIN      
        1 gatcacgcgc cccttttcat gcgcgcccac ggagacgccg ccgcccatgt gcgccacgac
       61 gaggttgcac tccgcgtacg gtttttgcag ctccgcggcg gctcttctgg ccacggcctt
      121 ctggttgagc gcgtggaaga tggagcggcg ctcgatctcc ggaagtccgg agtagcgtgc
      181 aagcggacac atttcgtcca cgacgaccgg gtcgacgatg tacgccttcc cgcatccgcc
      241 ctcgtcggcc agacgaacag ccagcggagc gcccaggttg ctcgcgtgcg cgccccacgt
      301 acaggcgcgc aggtcttcga gcatcttatc gctcacttcg tacgtgccgc tctcgatcgg
      361 cttgagaagt cctccccgcc ccactaccgc gtcgaggtcc gcgaccgttg cgccgtgcgc
      421 ggcaaggcac ttgcggatct cttcaaggcg gaattcctcc tgctccatga cgccggagaa
      481 acggccgatc acgtccgtat cgtagcgctg cgtttcgctc cagagttcgg tatggtcctc
      541 gaagagagcg accttggtgc tggtggagcc gggattgaga gcgaggattt tcataacgac
      601 gaacgctcta cttattaaag tcggagagcg tgaccgccgc ggcgatggag agcagcttcg
      661 cccttgtcga gtcggcgcgg ctggtgagga cgatgggggc ggaggcgccg aggatgagtc
      721 cggccgtctc gttcttcgag aagtagacga tccccttcgc gagcatgttc cccgcgtcga
      781 tgttcgggac gatgaagatg tcggcctttc cggcgacggg ggagacgatc cccttcgtct
      841 tggcggactc ctcgctcacg gcgttgtcga gcgcgaacgg gccgtcgacg atgcagttct
      901 ttatctggcc gcggcggttc atctgcgtga gcgcggcggc gtcaagggtc gcgggcatgt
      961 cggggttcac cacttcgacg gcggcgagga cggcgacctt gggctcggca acgccgaacg
     1021 aacgggcgag tccgacggtg ttctgggtga tatcggcctt cgcggcaaga tccgggtaca
     1081 tgttgaaggc ggcgtcggcg atgaagaaga tgcggtcgta cccttcgacg ctgtggaagt
     1141 agcagtggga gatggtgttc ttccccttcc ggagtccgac ttccttgttg agcatcgcgc
     1201 ggaggaagtt gttcgtgtgg atctggccct tcatgtagat gtcggccttc ttggaagaga
     1261 cctgggagac ggcggagatc cccatcgagg gctcgtcgcg ctcgtcgatg acctcgtagt
     1321 tggcgaggtc gactcctgcc tgctccgctg cagccttcat cttgt
//
LOCUS       DAOSWW010000843         1361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_88894_length_1361_cov_4.089587, whole genome shotgun sequence.
ACCESSION   DAOSWW010000843 DAOSWW010000000
VERSION     DAOSWW010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88894_length_1361_cov_4.089587"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PK442_13570"
     CDS             <1..989
                     /locus_tag="PK442_13570"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOO88727.1"
                     /translation="KRGILVVCALAFVFLFALPAAYAAVELKLGHFGPEGHPATIAAA
                     QFAENVEKRTNGEVKIVVYPNNALGSPPEVLEQVIMGVIDMSLSGQDQIAKHVKLFDS
                     VAIPFSMQGYEHADRILDGPFKEWAAPELEKIGLVYINSWEWGFRQITNSKRPILSPD
                     DVKGLKIRTPPAMAYQAAIEALGGNVLTIAFGELVMAMRQGVVDGQENPIGVIYDLKL
                     YESQKFISIVNYLYSSMTHVVSKKVFDSLTEEQRNIIIEESNKARLLMRELVRKQEQE
                     QIADMRAKGIQIDTPDLTSFQAAMGPAYEKIKKNIGEENFNKWMEMAKATAE"
     gene            1082..>1361
                     /locus_tag="PK442_13575"
     CDS             1082..>1361
                     /locus_tag="PK442_13575"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88728.1"
                     /translation="MLTFYIFATLIGFLLLNVPVAAAIGLTSVIFFAGLGQGAFLAML
                     PHRMYFGTTGFTLLAIPFFILAGNLMNTGGITRRIFRFASAMVGHIPGG"
BASE COUNT          295 a          383 c          395 g          288 t
ORIGIN      
        1 tgaaacgagg aattctggta gtgtgcgcgc tcgcgttcgt gtttctgttc gcgctccctg
       61 ccgcatacgc ggcggtcgag ctgaaactgg gtcacttcgg accggaagga catccggcca
      121 ccattgccgc cgcgcagttt gcggagaatg ttgaaaaacg gacgaatggc gaagtcaaga
      181 tcgtcgtgta cccgaacaat gctctcggga gtccccccga agtcctggaa caggtcatca
      241 tgggcgttat cgacatgagc ctctcgggac aggaccagat cgcaaagcac gtgaagctgt
      301 tcgactccgt ggccatcccc ttctcgatgc aggggtacga gcacgcggac cggatactcg
      361 acggcccgtt caaggagtgg gcggcccccg agctcgagaa gatcggcctc gtctatatca
      421 acagctggga gtggggcttc cggcagatca ccaactcaaa acgccccatc ctctccccag
      481 acgatgtgaa gggcctgaaa atccgcaccc cgcccgcgat ggcgtatcag gcggcaatcg
      541 aggcgctcgg cggtaatgtc ctcaccatcg ctttcgggga attggtcatg gcgatgcggc
      601 agggggtcgt cgacgggcag gagaacccca tcggcgtcat ctacgatctt aaactctacg
      661 aatcccagaa gtttatctct atcgtcaact acctctacag ctcaatgacg cacgtcgttt
      721 cgaaaaaggt cttcgattcg ctgacagaag agcagcgcaa catcatcatc gaggagtcga
      781 acaaggcccg tctgctgatg cgcgagcttg tccgcaaaca ggagcaggag cagatcgccg
      841 atatgcgcgc gaaggggatt cagatagata ccccggatct tacttccttc caggccgcaa
      901 tggggccggc gtacgaaaag atcaagaaga acatcggtga ggagaacttc aacaagtgga
      961 tggagatggc aaaggccaca gccgaatagc cagtcctcgc aatcgccggg ccgggcgtcc
     1021 gttttcgggc gcttggccct tctgttgtcg gatgcgcact acgtgaaacg ggggatactt
     1081 catgctcact ttttacattt ttgcaacgct tataggcttt ttgttattaa acgtgcccgt
     1141 ggcggcggcg ataggtctta cctcggtcat ttttttcgcg ggcctggggc agggcgcgtt
     1201 tctcgccatg ttgccgcaca ggatgtactt cggtaccacc gggtttacac ttttggcgat
     1261 tcctttcttc atcctcgcgg gaaacctgat gaacaccgga ggcatcacaa ggcgcatctt
     1321 ccgtttcgca agcgccatgg tgggacatat cccgggaggt t
//
LOCUS       DAOSWW010000844         1360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_89012_length_1360_cov_3.631418, whole genome shotgun sequence.
ACCESSION   DAOSWW010000844 DAOSWW010000000
VERSION     DAOSWW010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89012_length_1360_cov_3.631418"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK442_13580"
     CDS             <1..183
                     /locus_tag="PK442_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOO88729.1"
                     /translation="VVSREKLAMAALERTLGPFERSLDVHVSNLRRKLGPADDGSSRI
                     RTLRSEGYLLACAATH"
     gene            165..>1360
                     /locus_tag="PK442_13585"
     CDS             165..>1360
                     /locus_tag="PK442_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOO88730.1"
                     /translation="MRGYTLKLFWWFWLTAAATGLAHHLTSVITEGNIFREQMIANVQ
                     EIALVYGERVASKEAREGPEEAVRYLERLRRTHRFFGFLFGEDGTELTGQPKAPESIG
                     KIVDEARNTGAPVVRMERGRPLFAVPLEEFPGLVFAGRILNPFFESRSLPVLRLIAAA
                     LVAGLLSWFLAVRLAKPVRKLRGAVQRLAAGELGARAEHTAKRNDEIGQLAKDFNRMA
                     DRIEELLRGQRQLLADVSHELRSPLTRLGVGLELLRRRSASPDDEAFKRIETELGRID
                     NLIGSLLKLGRLEALEQPEKRTRIDLTALLRQICADAAFEAAPLGKRIETDFAEGALL
                     HADQALLRSAVENIVRNALRYTPQHTTGQDGGKVRVSLFRVPETERPLHILVEDEGPG
                     VPPGEL"
BASE COUNT          261 a          410 c          453 g          236 t
ORIGIN      
        1 gtcgtctcgc gggaaaaact ggcgatggcc gcgcttgaac gaacgctggg accgttcgag
       61 cgaagtctgg acgtccacgt cagcaacctg cgccgaaaac tcggaccggc ggacgacggt
      121 tcgtccagga tacggacgct ccgaagcgaa gggtaccttc ttgcatgcgc ggctacacat
      181 taaagctctt ctggtggttc tggctgaccg cggcggcgac gggactggcg caccatctga
      241 ccagcgtgat cacggaggga aatatcttcc gcgagcagat gatcgcgaac gtacaggaga
      301 tcgcgctcgt ctacggagag cgcgttgcat cgaaagaagc gcgggaaggc ccggaagaag
      361 cggtccgcta cctggagcgg ctccgtcgta ctcaccgttt tttcgggttt ctgttcggcg
      421 aagacggtac ggagctgacg ggacagccga aggcgccgga atcgatcgga aagatcgtcg
      481 acgaagcgcg aaacacaggg gcgcccgttg tacgcatgga acgggggcgt cctctttttg
      541 ccgtcccgct ggaggagttt ccgggactcg tcttcgcagg acgcattctc aatcctttct
      601 ttgaaagcag aagtcttcct gtgctgcgcc tcattgcggc cgcgctggtc gccggcctgc
      661 tctcgtggtt tttagccgtc cgcctcgcca aaccggtcag gaagctccga ggcgcggtgc
      721 agcggctcgc cgccggcgag ctcggcgcgc gggcggagca tacagccaag cggaacgacg
      781 agatcggcca gctggcgaag gacttcaacc gtatggcgga caggatagaa gagctgcttc
      841 gcgggcagcg tcagcttctt gccgacgtct cgcacgagct tcgatcgccg ttgacgaggc
      901 ttggcgtggg actcgaattg ctgcgccgcc gctccgcgtc gccggacgac gaagccttta
      961 aacgcattga gacggagctg gggcggatcg acaacctcat cggctccctg ctgaagctgg
     1021 ggcgcctcga agcgctcgaa cagccggaga agaggacgcg catcgacctg acggcgcttt
     1081 tgcggcagat ttgtgcggac gcggcttttg aagcggctcc cctcgggaag aggatcgaaa
     1141 cggacttcgc cgaaggagcg ctgctccacg cagaccaggc gcttcttcgg agcgctgtgg
     1201 aaaatatcgt ccgcaacgcg ctccgctaca ccccgcagca cacaacaggg caggacggag
     1261 ggaaggtgcg agtctccctc ttccgcgttc cggagacaga gcggccgctt cacatcctgg
     1321 tcgaagacga aggccccggc gttcctcccg gcgagctgga
//
LOCUS       DAOSWW010000845         1360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_89027_length_1360_cov_2.794636, whole genome shotgun sequence.
ACCESSION   DAOSWW010000845 DAOSWW010000000
VERSION     DAOSWW010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89027_length_1360_cov_2.794636"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1360)
                     /locus_tag="PK442_13590"
     CDS             complement(<1..>1360)
                     /locus_tag="PK442_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOO88731.1"
                     /translation="LLALITFSIIKLVAPLRGMTRAIRRFSETDSFLSAKRSSPSNTP
                     SDRRMSLLSDALEQLKTDIESAAAPEKKDELAILSSALASMSALLRSRMRVQEAMTDI
                     MNVAAVSRTKDDLAWNFTRLVMEETDSCMAAFYIRNEAEPGKLDLVASVGLPPDTLKS
                     ITPSSLEGQFGAPVLASEVLLLDIPPARSPVFHTIAGQLLPAQLLTLPINVREEPVAL
                     FAIGSLTPYPEEYKHAFSIVREGLNAAMANLLAGERERELVQDLQAANQELAAQSEEL
                     EYQARELENQNAELNIQRRKSEEASRLKTEFLSNMSHELRTPLNSILALSRVLRAEGK
                     ERLTDEEQEYLNIIERNGKNLLMLINGILDLSRIEAGREDLHMKETQASRLLLETAES
                     MRPLAREKGISIDCDFPQELPCIITDEEKLRRIIINIAGNAVKFTETGGVRFCATADG
                     NAV"
BASE COUNT          224 a          363 c          459 g          314 t
ORIGIN      
        1 atacggcgtt gccgtcggca gttgcgcaga agcgtacgcc gcctgtctcg gtgaacttga
       61 ccgcgttgcc cgcgatgttg atgatgatgc ggcgcagttt ttcctcgtcg gtgattatgc
      121 aggggagctc ctgcgggaag tcgcagtcta tcgagatgcc cttttcccgg gcgagcgggc
      181 gcatactctc cgccgtttcc aggagtagac ggcttgcctg agtttctttc atgtggaggt
      241 cttcgcgtcc ggcttctatc cttgagagat cgaggatgcc gttaatgagc atgaggaggt
      301 ttttcccgtt ccgctcgatg atattgaggt actcctgctc ctcgtctgta aggcgttctt
      361 tcccctctgc gcgcagcacc ctggaaagcg ccagaatgga gttgagcggc gttctgagtt
      421 cgtggctcat gttggagagg aactccgttt tcagcctgct ggcttcctcc gattttcttc
      481 tctggatgtt cagctcggcg ttctggtttt cgagttcgcg agcctggtac tccagctcct
      541 ccgactgcgc ggcaagctcc tggttggcgg cctgaagatc ttgaaccagc tcgcgctccc
      601 tctctccggc aaggaggttt gccatcgcgg cgttcaagcc ctcccggacg atggaaaatg
      661 cgtgtttgta ctcctccgga tagggggtga gcgagccgat ggcgaaaagg gcgaccggtt
      721 cctcacggac gttgatgggg agggtgagca gctgcgcggg gaggagttgc ccggcgatgg
      781 tgtggaaaac gggcgaacgc gccggaggga tgtcgaggag caggacctcg ctcgcgagga
      841 ccggcgcgcc gaactgtcct tccagggagg atggggtgat ggatttcagt gtgtccggag
      901 gaaggccgac ggacgccacc agatcgagct ttcccggctc cgcttcgttg cggatgtaga
      961 aggccgccat gcaggaatcc gtctcttcca tgacgagccg tgtgaagttc catgccaggt
     1021 cgtcttttgt ccgcgacacc gcggcgacgt tcatgatgtc ggtcatcgcc tcctggaccc
     1081 gcatgcgtga acgaagcagc gcggacatgg aggcgagggc ggatgagagt atggcaagtt
     1141 cgtctttctt ctctggggcg gcggcgcttt caatgtcggt tttcagctgt tcgagcgcgt
     1201 cgctgagaag ggacatcctt cggtcggagg gggtattcga gggtgaactc ctctttgcgg
     1261 aaaggaagga gtccgtctcg gagaagcggc gtatggcgcg cgtcatcccg cgcagcggcg
     1321 cgaccagctt gatgatgctg aatgtgatca gcgcgaggag
//
LOCUS       DAOSWW010000846         1359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_89110_length_1359_cov_3.641871, whole genome shotgun sequence.
ACCESSION   DAOSWW010000846 DAOSWW010000000
VERSION     DAOSWW010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89110_length_1359_cov_3.641871"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1030)
                     /locus_tag="PK442_13595"
     CDS             complement(146..1030)
                     /locus_tag="PK442_13595"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOO88732.1"
                     /translation="MKTYKYYKLNAFVKGNSEGNPAAFLDLGDETLSDESMQRIAKEH
                     AGFVSEVVYARKNEEREIFLTYYSSECEVAFCGHGTIATMYEIIRRDDALRSAKVIEI
                     CTNKKGRLNIYNHIEEEDCIYISAPDPVMHGCTLSRASIAEALGIREEAISDALPVDI
                     IDAGLRTLLVPIRALEQEVSMYPDEQALKSYCLHNDVDIVLVFSMETAEPGYFAHTRV
                     FAPKFGYLEDPATGSGNSAFGYYLLKNKKWSGDAVRIEQGGDNRIFNEVKLMTTDGKV
                     LFGGKAALKIEGNYFVGS"
     gene            complement(1027..>1359)
                     /locus_tag="PK442_13600"
     CDS             complement(1027..>1359)
                     /locus_tag="PK442_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88733.1"
                     /translation="MHWANVDEEQLEREREVPTSFGETLSWDYYEYVRAHPVDRWDSP
                     TSILYGENDNLTERCVLDAFAQKFACGVTVMSGGEHYFHTPEQLTFLKNWIREQNGDG
                     GISKEGKR"
BASE COUNT          239 a          420 c          366 g          334 t
ORIGIN      
        1 gggcgagggc ttcggcggcg catgttttgc tgccgggccc ctgacgcggc aggttttcga
       61 agacttcgaa gaagagctcc cagaagcgcg gtgagtggct ctccatgatc attctcctcc
      121 tcgttccgcc ccggctccag ggcgcttagc tgccgacaaa atagttccct tcgattttca
      181 gcgcggcttt tccgccgaac aggacctttc cgtccgtcgt catcagcttt acttcgttga
      241 agatacggtt gtcgccgccc tgctctattc tgacggcgtc cccgctccac ttcttgttct
      301 tgagcaggta gtagccgaac gcgctgttcc ccgaccctgt ggcggggtcc tccaggtagc
      361 cgaacttcgg ggcgaacact cgggtgtggg cgaagtaccc gggctccgcc gtctccatcg
      421 aaaagaccag cacgatatcc acgtcgttgt gaaggcagta ggatttcaga gcctgctcgt
      481 cggggtacat cgacacctcc tgctccagag cgcgtatcgg cacgagcagc gtccgaagcc
      541 ccgcgtcgat gatgtcgacg ggcagcgcat ccgaaatggc ctcttcacgg atgcccaggg
      601 cttcggcgat cgacgcccgt gaaagtgtgc atccgtgcat caccggatcc ggcgcggaga
      661 tatagatgca gtcctcttct tcaatatgat tgtagatgtt cagcctgccc ttcttatttg
      721 tgcaaatttc gatgaccttc gcgcttctca gggcatcgtc ccgccggatg atctcgtaca
      781 tggtcgctat cgttccgtgc ccgcagaacg cgacttcgca ctccgaggaa taataggtca
      841 gaaaaatctc ccgctcctcg ttttttcttg catacaccac ttccgacacg aagcccgcat
      901 gctccttcgc gatgcgctgc atgctctcgt ccgagagcgt ttcgtcgccg aggtcgagaa
      961 aagcggccgg atttccttcg gagttcccct ttacgaaggc gttgagcttg tagtatttat
     1021 acgttttcat ctctttccct ccttggaaat gcctccgtcg ccgttctgct cacgtatcca
     1081 gttcttcagg aaagtgagct gctcgggagt gtggaagtag tgctcgccgc cgctcatgac
     1141 cgttactccg caggcgaatt tttgcgcgaa ggcgtcaaga acgcatcgtt ccgtcagatt
     1201 gtcgttctct ccgtagagta tcgaggtcgg gctgtcccag cggtcgaccg ggtgcgcccg
     1261 cacatattca tagtagtccc aggagagcgt ctcgccgaag gatgtcggta cctcccgctc
     1321 tctctccaac tgttcttcat ccacattcgc ccagtgcat
//
LOCUS       DAOSWW010000847         1359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_89190_length_1359_cov_1.694785, whole genome shotgun sequence.
ACCESSION   DAOSWW010000847 DAOSWW010000000
VERSION     DAOSWW010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89190_length_1359_cov_1.694785"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PK442_13605"
     CDS             <1..705
                     /locus_tag="PK442_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOO88734.1"
                     /translation="ADWLTTPGADEETDDALRESIALRNRERRALQSLWELSALDEPP
                     LSGLEVHQFGEYMQYRFDKRKDVPWLEEQVRSIRAAWEGGERREHGKKPRIMITGCPT
                     GGVVKVIEAIENAGAVVVCYENCGGQKELGYLVDETKDPMEALAEKYLAIGCSIMSPD
                     TNRMNSLRALAERYRIDGVVEVLLTACHTYAVESYSVRNLVQSLGKKYLLVETDYSEG
                     DKEQIATRMDAFVEMM"
     gene            742..1347
                     /locus_tag="PK442_13610"
     CDS             742..1347
                     /locus_tag="PK442_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO88735.1"
                     /translation="MLKKLGIMALAVFLMAGATFASDLEPSGKGMRVWFDAGGSAGEP
                     YSTILINGAKQAAADLGVELTIVYSGWNAEKMLENFKNGLAARPTGYVVIGVPGDDGF
                     APLVDEAFADGMIVTAVDTELPRLQAKYQSRGFGYIGPDGWKQGEDMAKEILRRKEFK
                     PGDRAFVWGLKRLESRGRRARAILEVFEKAGLKVDYLEISD"
BASE COUNT          289 a          361 c          479 g          230 t
ORIGIN      
        1 gcggactggc tgaccacgcc cggggcggac gaggaaacgg acgacgccct gcgcgagtcc
       61 atcgccctca gaaacagaga gcgaagagcg ctgcagtccc tctgggagct gagcgcgctg
      121 gacgaacctc ctctttccgg tcttgaggtg catcagttcg gcgagtacat gcagtatcgc
      181 ttcgacaagc ggaaggacgt tccctggctc gaggaacagg tacggagcat ccgcgccgcg
      241 tgggagggcg gcgagcgtag ggagcacgga aagaagccga ggatcatgat caccgggtgt
      301 ccgacggggg gcgtcgtgaa ggtgatcgag gccatcgaga acgctggggc cgtcgtggtc
      361 tgttacgaga attgcggcgg acagaaggaa ctcggctacc ttgtggacga gaccaaagac
      421 cctatggagg cgcttgcgga gaagtacctc gccataggct gctccatcat gagccccgat
      481 acgaaccgca tgaattcgct gcgtgcgctc gcggagcgct acaggatcga cggcgtggtc
      541 gaggtgctgc tcaccgcctg ccatacgtac gcggtggagt cctattccgt gcggaatctt
      601 gtgcagtccc tcgggaaaaa gtacctgctc gtggagacgg actattcgga gggcgacaag
      661 gagcagatcg ccacgcggat ggacgccttc gtcgagatga tgtaaccccc gcgttcgcgg
      721 gaaaacgaaa ggagaagaga gatgttgaaa aaactgggaa tcatggcgct tgcggtcttt
      781 cttatggcag gggcgacgtt cgcgtccgac ctcgaaccga gcggcaaggg gatgcgggtc
      841 tggttcgacg cgggcggttc ggcgggcgag ccgtactcca caattttgat caacggcgcg
      901 aagcaggcgg ccgccgacct cggagtcgaa ctgacgatcg tctactccgg ctggaacgcg
      961 gagaagatgc tggagaactt caagaacgga ttggccgccc gcccgacggg gtatgtcgtg
     1021 ataggcgttc cgggagacga cggattcgcc cccttggtcg acgaggcgtt cgccgacggc
     1081 atgatcgtaa cggctgtgga caccgaactt ccgcgccttc aggcgaagta tcagtccaga
     1141 ggtttcggct acatcggccc cgacggctgg aagcagggcg aggatatggc gaaggagatc
     1201 cttcggcgca aggagttcaa gcccggagac agggcgttcg tctggggtct gaagcgtctc
     1261 gaaagcagag gtcgcagggc gcgggcgata ctggaggtct tcgaaaaggc cgggctgaag
     1321 gtggactatc tggagatttc cgactaggtc aataaggac
//
LOCUS       DAOSWW010000848         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_89450_length_1356_cov_3.718678, whole genome shotgun sequence.
ACCESSION   DAOSWW010000848 DAOSWW010000000
VERSION     DAOSWW010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89450_length_1356_cov_3.718678"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PK442_13615"
     CDS             complement(<1..967)
                     /locus_tag="PK442_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88736.1"
                     /translation="MKRRYMKQQWTLRFKKLGLIEEGAVRIAPLMIFTGDNNTGKSYV
                     MTLLWGVIALGRSLFPTSIPESDSYRQCEKWLGSFSQEGEDEKMLLPEDEEVFVRWFG
                     DVLNQRRQTLCDELFGENAVRLRELRITDFVRERPLSLRWNKDAAEGSRYSSGEHYVR
                     FPLPSSPPPRAEVYRMIQYLTWKLIMDDLSAPLYPPRSPVKTVPPGEILYLPAARTGF
                     VLLRKALVSAALSMGGESVPLLLPVRRFVQRLLTLSFKEKNRFSAFADILERRILRGH
                     IHPDGAPLPEYRYSPQGQDNEALPLWRTSSLVTELAPLLVFLRGND"
BASE COUNT          299 a          389 c          374 g          294 t
ORIGIN      
        1 cgtcgttccc tcgcagaaag acaagaagcg gagcaagctc cgtaacaagc gacgacgtcc
       61 gccaaagagg gagcgcttcg ttatcctggc cttgaggact gtatcggtac tccggtagcg
      121 gagcgccgtc cgggtgaatg tgtccgcgca gaattcggcg ctccagaata tccgcgaagg
      181 cggagaacct gtttttctct ttgaaggaaa gggtaagaag gcgttgaacg aaacgcctga
      241 cgggcagcag aagcggcacg ctctcgcctc ccatgctcag cgctgcggaa acaagagctt
      301 ttcgcaagag cacgaaccct gttcgtgccg ccggcaagta gaggatctcg cccgggggaa
      361 cggttttcac cggagatcgc ggaggataga gaggcgcgct caaatcgtcc atgatcagct
      421 tccaggttaa atactggatc atcctgtaga cttcggctcg gggaggagga ctcgacggca
      481 gcgggaagcg gacatagtgt tctccgctgg aataccggct tccctcggcc gcgtctttgt
      541 tccatcgaag ggaaagaggc cgttcgcgaa caaaatccgt aatgcgaagc tcgcgaagcc
      601 gcactgcgtt ttccccgaaa agctcgtcgc agagagtctg tcgccgctgg ttgagcacgt
      661 ctccgaacca acgtacgaaa acctcctcgt cttcggggag cagcattttt tcgtcttcac
      721 cttcctgcga aaaactcccc agccactttt cgcactggcg atacgagtcg ctctccggaa
      781 tcgacgtcgg gaagaggctg cgtccgagcg cgatcacccc ccagagcaga gtcatgacgt
      841 agctttttcc ggtgttgtta tcgccggtaa aaatcatgag aggagctatc cggacggctc
      901 cttcctctat cagtccaagt tttttgaagc gcaaggtcca ctgctgcttc atatatcgac
      961 gtttcatgga acaccctctt ttcagggagc atagaatagc atgctaaaat cgaaatttat
     1021 tataccatcg acttcgatcc cgcgtgtcgg ctatggattt tccgagcaat aaaaaggccc
     1081 ccgaggtttg gagaagtcgc cgggctacgc aacgctcttc ccaaaccacc gagggcgtgt
     1141 caattctacg gtaaattccg aacttgttct atcgaatgct ttcattgctc ctcttcgccg
     1201 aaccctcgac aactgaactc gctttctccg cgcctcccgt tcactcgctt tcggcgagct
     1261 gcgaaacctc cgccaggacg ctccgtatgg cggctgcgga acggcggatg tcctcttcgg
     1321 tcgtggacca gttggagacc gagatgcgca tggcgg
//
LOCUS       DAOSWW010000849         1353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_89771_length_1353_cov_5.411402, whole genome shotgun sequence.
ACCESSION   DAOSWW010000849 DAOSWW010000000
VERSION     DAOSWW010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89771_length_1353_cov_5.411402"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1353
                     /locus_tag="PK442_13620"
     CDS             <1..>1353
                     /locus_tag="PK442_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO88737.1"
                     /translation="NNRTDAEKDTEAKDGHSVFEASLLRADGSLLPVEVHRRSFTFGA
                     LPLSLLLARDLKERKQFEERLARKAYFDDLTGLPNRHSLLKAINRLLNDGTRTRMFSL
                     AVINMDHFKLVNAQVGNLNGDRILLLIAEKIKELQEPEAQLFRTSGDEFALLFPLPEG
                     GESEEMLLMDRIRKGIATPCEVGDETVFPSASIGVLVDIAEGKNASGAIDAALHAVTQ
                     ARKNGIGRIARYVAPEVREYTRGPNLLTLQAELQVALERHEFLPYFQPIYDLDSEKIC
                     GFETLARWMRQDGGMLAPCDFVPQAEHTGFIPNIDICMMEHALRAIVRTQDMARTSRL
                     FFSANGSPLFFRGLASMETVEYLIDTTGADPKLFTIEVTESALIDNLDDVHRKLDYLK
                     SCGVKIALDDFGTGYSSLQYINHLPFDYLKLDRAFVSQLFSSEKTEHMMRTIIGLAHN
                     L"
BASE COUNT          287 a          435 c          393 g          238 t
ORIGIN      
        1 aacaatagaa cagatgcaga aaaggatacg gaggcgaagg acgggcacag cgtcttcgag
       61 gcgtcgctcc tcagagcgga cggttcgctg cttcccgtcg aggtgcaccg ccggagcttc
      121 actttcggcg cgctcccact gtcgcttctt ctggcgcgcg acctgaagga gcgcaagcag
      181 ttcgaggagc gcctcgcacg gaaagcgtat ttcgacgatc ttaccgggct gcccaaccgt
      241 cactcgctcc tcaaagcgat caaccgcctg ttgaacgacg gaacccgaac gcgcatgttc
      301 tcgctcgccg tcatcaatat ggaccacttc aagctggtca acgcccaagt cggcaacctg
      361 aacggcgacc ggattctcct gctgatcgcc gagaagatca aggaacttca ggagcccgag
      421 gcacagctct tcagaacgag cggcgacgaa ttcgcgctgc tcttccccct tcccgagggc
      481 ggcgaaagcg aggagatgct cctgatggac cgtatccgca agggcatagc gacgccgtgc
      541 gaggtggggg acgagaccgt ctttccctcg gccagtatcg gagtgctcgt cgatattgcg
      601 gaggggaaga acgcctccgg ggcgatcgac gccgcgctgc acgccgtgac gcaggccagg
      661 aagaacggca tcggccggat cgcccgctac gttgcgccgg aagtcagaga gtacacgcgc
      721 ggtccgaact tgctcacgct ccaggcggaa ttacaggtcg ccctggagcg ccacgagttc
      781 cttccctact tccagccgat ctacgacctg gattccgaga aaatatgcgg cttcgagacc
      841 ctcgcccgct ggatgcgcca agacggagga atgcttgctc cctgcgactt cgtcccgcag
      901 gcggagcata ccggtttcat cccgaacatc gatatctgca tgatggaaca tgcgctccgc
      961 gcgatcgtgc ggacacagga catggcgcgc acctcgcggc tcttcttctc ggcgaacgga
     1021 tcgccgctct tcttccgcgg actcgcctcg atggagactg tagagtacct catcgacacc
     1081 accggcgcgg acccgaagct cttcaccatt gaagtgaccg agagcgcgct gatcgacaac
     1141 ctcgacgatg tgcacaggaa gctggactac ctgaagagct gcggcgtcaa gatagccctc
     1201 gacgacttcg ggacgggcta ctcctccctc caatacatca accatctgcc gttcgactac
     1261 ctcaagctgg acagggcgtt cgtcagccag cttttcagct cggagaagac cgaacacatg
     1321 atgcgaacga tcatcggcct ggcgcacaat ctg
//
LOCUS       DAOSWW010000850         1353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_89797_length_1353_cov_3.167951, whole genome shotgun sequence.
ACCESSION   DAOSWW010000850 DAOSWW010000000
VERSION     DAOSWW010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89797_length_1353_cov_3.167951"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PK442_13625"
     CDS             <1..1149
                     /locus_tag="PK442_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO88738.1"
                     /translation="IICTLFALYSYIYFKLRSAIGPGWKWTALYTLLSFFIIMGSRIL
                     WIVNLEAYPGLRKILSCSVYMGMAFFFILFTAFFFLDLLRFLVWLTDLLLSACFSDLF
                     PSPKMRAVLAIGFAFFACTYGWFEALAVRPVYITIATDRLPRGVDKLRIAHITDVHLG
                     WVIQEERLGRILDVVRAAQPDMLVSTGDLVDGDMEEREAEVALFRGLNLPLGMYAVTG
                     NHEYYAGIDQAIAFKEHAGMQLLRNEALRVGGIILAGMDDPTALRFGGTPTPEIDVLA
                     PLPENRFVLLLKHQPTVDPDSIGLFDLQLSGHTHRGQIWPFYWLTRMVYEYRPGLRAL
                     APSKSGEAYASGAARESRVFVSNGSGTWGPPIRFFAPPQVGIIDIVRK"
     gene            complement(1185..>1353)
                     /locus_tag="PK442_13630"
     CDS             complement(1185..>1353)
                     /locus_tag="PK442_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HOO88739.1"
                     /translation="LLKGVVEGLWRERDLPVCAACTELPLAYDASGLPQERTVSSLGA
                     LCGACLRRIYW"
BASE COUNT          249 a          459 c          377 g          268 t
ORIGIN      
        1 attatctgca cgctctttgc gctgtactcc tacatctatt tcaaactgcg cagcgccatc
       61 ggtcccggat ggaagtggac ggcgctctac acgctgctct cctttttcat catcatgggg
      121 tcgaggatcc tctggatcgt caacctggag gcgtacccgg gactcaggaa aattctttcc
      181 tgctccgtct acatgggaat ggccttcttc ttcatcctgt tcacagcctt ctttttcctc
      241 gacctcctgc gcttcctcgt ctggctgact gatctgctgc tctccgcctg cttcagcgac
      301 ctcttcccct ccccgaaaat gcgcgcggtg ctcgcgatcg gattcgcttt cttcgcctgc
      361 acctacggct ggttcgaagc ccttgcagtt cgccccgtgt acatcactat cgccactgac
      421 aggctcccca ggggagtgga caaactccgg attgcccaca tcacggacgt gcatctgggc
      481 tgggtcatcc aggaagaacg gctcggccgc atccttgatg tggtaagagc cgcgcagccc
      541 gacatgctgg tgagcaccgg cgaccttgtc gacggcgaca tggaggagcg cgaggccgag
      601 gttgcactct tcagagggct gaatctgccg ctggggatgt acgccgtcac cggaaaccac
      661 gaatactacg cgggcatcga ccaggcgatc gccttcaagg agcacgccgg aatgcagctg
      721 cttcgcaacg aggcgcttcg tgtcggcggc atcatcctgg cgggaatgga cgaccctacg
      781 gcgctacgct tcggcggaac gcccacaccg gagatcgacg tcctcgcccc gcttcccgag
      841 aaccgtttcg tcctgctcct caagcatcag cccacagtgg atccggattc catcggtctt
      901 tttgatctcc agctctccgg ccacacgcac agaggacaga tctggccctt ctactggctt
      961 acaaggatgg tctacgagta caggccggga ctacgcgccc tggcgccgtc gaagagcgga
     1021 gaggcctacg cctccggcgc cgcccgcgag agccgcgtct tcgtcagcaa cggatcggga
     1081 acgtgggggc cgccgatacg cttcttcgcg ccgccccaag taggcatcat cgacatcgtg
     1141 agaaaataaa aaagcgcccc cgtgtcgaac agggggcgct tttgctacca gtagatcctt
     1201 cgaagacagg ccccgcagag cgcccccagg ctggagacgg ttctctcctg cggcagcccc
     1261 gaggcgtcat aggcgagagg cagctccgta cacgccgcgc acaccggaag atcgcgctcg
     1321 cgccacagcc cctccacaac gcccttcagc agc
//
LOCUS       DAOSWW010000851         1349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_90229_length_1349_cov_3.418083, whole genome shotgun sequence.
ACCESSION   DAOSWW010000851 DAOSWW010000000
VERSION     DAOSWW010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90229_length_1349_cov_3.418083"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PK442_13635"
     CDS             <1..704
                     /locus_tag="PK442_13635"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88740.1"
                     /translation="RVGFAPFRQIGVGIACAVVGNIIGITNLTGLGLVLGDNIVSLAG
                     GSVLITTFLTMIVCVILGMGLPTTACYIVTATIAAPALMKLGVNPLAAHMFTYYFACL
                     SNLTPPVAIASYGAAGLSGQSPSAVGWTGFRISLAGFLIPFTFIFNPELLLVGGDYVS
                     ITISTITSIIGVIALASSLQNFLYVRMNLIQRAGMFFGALLLIFPGLTTDIAGVALTG
                     TVFFWQRMQARSRAL"
     gene            735..>1349
                     /locus_tag="PK442_13640"
     CDS             735..>1349
                     /locus_tag="PK442_13640"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOO88741.1"
                     /translation="MKTKMKSGKEEIFLSIPDKNVIAVLEPSGGSPVGDLGEEVRRLL
                     AAPTAGPSLEEIVLKGPARSVAVIVNDMTRSTPTASMLPPLLEELERLGVGREKVTVA
                     VATGTHRPMTDDEIRVVLGDAVFGTVKVENHDCDSPDLVDMGILSTGNRLLINKTVAE
                     ADLRIAIGEVLLHYYAGFAGGRKSILPGVAGRETVMNNHKMMTEP"
BASE COUNT          240 a          397 c          416 g          296 t
ORIGIN      
        1 accgggtcgg ctttgcgcca ttccgccaga taggcgttgg tatcgcctgc gccgtcgtgg
       61 gcaatatcat cggcattacg aacctgacgg ggctggggct tgtgctcggc gacaacatcg
      121 tctcgctcgc gggcggcagc gtcctcatca cgaccttcct cacgatgatc gtctgcgtca
      181 tcctcggcat ggggttgccg accaccgcgt gctacatcgt taccgcgacc atagcggctc
      241 ccgcgctgat gaagctgggc gtcaatcctc tggctgcgca catgttcacc tactacttcg
      301 cctgtctctc gaacctgacg cctccggtgg ccatcgcctc ctacggggcc gcgggacttt
      361 cggggcagag cccctccgcc gtgggctgga cgggtttcag aatttcgctt gccggttttc
      421 ttatcccctt cacgttcatc ttcaaccctg aacttctgct cgtggggggc gattatgtct
      481 ccatcaccat ctcgacgatt acgagcatca tcggggttat cgcgctcgct tcgtcgctgc
      541 agaatttcct gtatgtccgg atgaatttga tccaacgggc gggtatgttc ttcggcgcgt
      601 tgctgcttat cttcccggga ttgaccacgg atatcgccgg agtagcgctg acaggaacag
      661 ttttcttctg gcagaggatg caggctcgtt cgcgcgccct ctgaagcttg gtgagatgag
      721 ggagggtgta cggaatgaaa acaaagatga aatcgggcaa ggaagaaata tttctctcca
      781 tccccgataa gaacgtcatc gcggtccttg aaccttcggg cgggagtccg gtcggggatc
      841 tcggggaaga ggtgcgcagg ctgcttgccg cgccgaccgc ggggccttct cttgaggaga
      901 ttgtcctgaa aggtccggcg cgatccgtgg cggtgattgt caacgacatg acccgttcca
      961 cgccgactgc ttcgatgctt ccgccgctcc ttgaagagct tgagcgtctc ggcgtcgggc
     1021 gcgagaaggt gacggtcgcc gtcgccaccg ggacgcaccg gccgatgacc gacgatgaga
     1081 ttcgcgtcgt tctcggcgac gccgtcttcg ggacggtgaa ggtggaaaat cacgactgcg
     1141 actccccgga cctggtggat atgggaatcc tctccaccgg gaaccggctg ctcattaata
     1201 aaaccgttgc ggaagcggat ctgaggatcg ccatcggaga ggtgctgctg cactactacg
     1261 ccggatttgc cggcggtcgg aagagcatcc ttcccggagt tgcagggcgc gagacggtga
     1321 tgaacaacca caagatgatg accgagccg
//
LOCUS       DAOSWW010000852         1348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_90354_length_1348_cov_3.122196, whole genome shotgun sequence.
ACCESSION   DAOSWW010000852 DAOSWW010000000
VERSION     DAOSWW010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90354_length_1348_cov_3.122196"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>1348
                     /locus_tag="PK442_13645"
     CDS             62..>1348
                     /locus_tag="PK442_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOO88742.1"
                     /translation="MILFHAPVGEHFSFGMFFPESAMNSFVRDISSLHLFGGFAIILL
                     ALVLLLPLTHEILSSFSSLSSTIDSIAAKLSDNKDFTETAFNVHLLAAEVGESMTRTS
                     LLEFQTFLGSMENALKTIGRQGEEIAALTEEALAIQDNLTEANGELNRRQKIWRDTLE
                     VMETMTMSGESEKNLRSVAENILKSTQAFGVLLSCRRGDRFFNLVCAGYGGASLPSGD
                     AIAIFGSVAGRAVREEDAVWVEDVARDREYVPVHPSVVSEVEIPLLHRGKANGILEVA
                     FDRRTPRNDDLLETLLPVASALGSLIEAENAHIEIKDSYRYLVRKLQSVTEIYHLESA
                     DHMDRIGAYSRLAARILGKNEEEQEDIEVFSRLHDIGKLRVPLSILAKPGKLTDEEMK
                     IVRNHPLWGAELIGGAKWLDMARKICLSHHEKWDGSG"
BASE COUNT          312 a          352 c          393 g          291 t
ORIGIN      
        1 cggcgaaggc atcctggaga gggaatccgg ggctttcagc ctaacgttca agggagagag
       61 gatgattctc tttcacgccc cggtcggcga gcatttttcc ttcggaatgt tcttccccga
      121 gagcgcgatg aacagctttg tcagggatat atcttccctt catctcttcg gagggttcgc
      181 aatcattctg ctcgctcttg tcttgctgct tcctctaacg catgagattt tgagttcttt
      241 ttcttcgctc tcctcgacga tcgacagtat cgccgccaaa ctctccgaca acaaggactt
      301 caccgaaacc gcgttcaacg tccacttgct ggctgctgaa gtgggggaat ctatgacgcg
      361 cacgtcgctt cttgagtttc aaacattttt ggggagtatg gagaatgctc tgaagacgat
      421 cggacggcag ggcgaagaga ttgccgcgct taccgaggag gctcttgcga ttcaggacaa
      481 cctcacagaa gccaacggag agctgaatcg gagacagaaa atatggagag atactctcga
      541 ggtcatggaa acgatgacca tgtcgggcga gtcggagaaa aacctccgaa gcgtcgccga
      601 gaacatactg aagagcacgc aggctttcgg ggtgcttctt tcctgccgaa gaggcgatcg
      661 ttttttcaac ctcgtctgtg cgggatacgg cggcgcttcg ctcccgtccg gcgatgcaat
      721 agcaatcttc ggaagcgtcg cgggcagagc tgtgcgtgag gaagatgccg tttgggtcga
      781 agatgtggct cgtgaccgcg agtatgttcc cgtgcatccg agcgtggtca gcgaagtcga
      841 aatccccttg ctccaccgcg ggaaggcgaa cgggattctc gaagtggcct tcgacaggcg
      901 aacacccaga aacgacgatc tgctcgaaac gcttctcccc gtggcgtccg cgcttggaag
      961 cctgatagaa gccgagaatg cgcacatcga gataaaagac tcctatcgct acctagtccg
     1021 taagcttcag tcggtaacgg agatctatca cctcgaatcg gcggaccaca tggacagaat
     1081 aggggcgtac tcccgtctcg cggcgcgcat tttgggcaag aacgaagaag agcaagagga
     1141 tattgaggtg ttttccaggc tgcacgacat aggaaaactt cgcgtgccgc tctcgattct
     1201 ggcaaaaccc ggaaaactca ccgacgaaga gatgaagata gttcgcaatc atccgctctg
     1261 gggggcggag ctgatcggcg gcgcgaagtg gcttgatatg gccaggaaga tctgtctctc
     1321 ccatcatgaa aagtgggacg gcagcggc
//
LOCUS       DAOSWW010000853         1342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91070_length_1342_cov_2.927739, whole genome shotgun sequence.
ACCESSION   DAOSWW010000853 DAOSWW010000000
VERSION     DAOSWW010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91070_length_1342_cov_2.927739"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PK442_13650"
     CDS             complement(<1..796)
                     /locus_tag="PK442_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid metabolism regulator protein"
                     /protein_id="HOO88743.1"
                     /translation="MASSQRLEIIRRTYYDSVTLMLVAKELLKLEGVSVATLNMGTEA
                     NYKIMGAGGFDLTGVDATPNDLVLALLSDAPEAEKVLDDALAKAKEYLANPPGRKKDS
                     GDNDYRPKSLDGALSVLPEANLAIVSVAGRYAGDVALQCIEKGLNVMLYSDNVTVEKE
                     IEVKKAAAAKGLLVMGPDCGTIIIRGVAMGMANACPTGPVSIVAAAGTGLQEVHVQLA
                     RRGVGVLHGIGTGGRDVKKEVGGIMCLQAVDTLLADNEVKVLAVLGK"
     gene            complement(812..>1342)
                     /locus_tag="PK442_13655"
     CDS             complement(812..>1342)
                     /locus_tag="PK442_13655"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HOO88744.1"
                     /translation="WLTDLLPPLRQGELLSLPGEGIPARYDPSLPAGRMNLRWAPLVR
                     EWRSLLEEELEPLAAPARDRRFGDLLGLGPGSTPAGDDYLAGYTTGRLWLGCSAPSLP
                     DTSRTTWLSAEILRDAAGGLTWKRSKDVLVSLSLNDAAALMNAVSRILDWGHTSGRAW
                     LAGLSAAVSDTIERNV"
BASE COUNT          245 a          452 c          395 g          250 t
ORIGIN      
        1 gcttgccgag cacggcgagc accttcactt cgttgtcggc gaggagtgtg tccaccgcct
       61 gcaggcacat gatgccgccg acctcttttt tcacatctcg cccgccggta ccgatgccgt
      121 gaaggacgcc gacgcccctg cgggcaagct gcacatgcac ttcctgcaac cccgtcccag
      181 ccgcggccac aatgctcacg ggcccggtcg gacaggcgtt cgccatcccc atcgcaacgc
      241 cgcggatgat gatcgtgccg cagtcgggcc ccatcacgag cagtcccttc gcggcggccg
      301 ccttcttgac ctcgatttcc ttctcgaccg tgacgttgtc ggagtagagc atcacgttga
      361 gccccttctc gatgcactga agggcaacgt cgcccgcgta gcgcccggct acagagacaa
      421 tcgcgagatt ggcttcggga aggaccgaga gcgccccgtc gaggctcttc ggcctgtagt
      481 cgttgtcgcc cgagtccttc ttccgccccg gagggttcgc aaggtactct ttcgccttcg
      541 caagggcgtc gtcgaggacc ttttcggcct ccggcgcgtc gctcaacaga gcgaggacaa
      601 gatcattcgg cgtagcgtcc actccggtca ggtcgaaacc gccggctccc ataatcttgt
      661 agttcgcctc cgtccccatg ttcagagtgg cgacggagac tccctccagc ttgagcagct
      721 ccttcgccac aagcatcagg gtgacgctgt catagtacgt tcttcggatg atttcgaggc
      781 gctgggaaga tgccacagta tcatcctcct ttcatacgtt gcgttcgatc gtgtccgaaa
      841 cggctgcgga aagacccgcg agccacgctc ttccggaagt gtgcccccag tcgaggattc
      901 gtgagacggc attcataagc gcagccgcgt cgttcagcga aagagatacc agcacatctt
      961 tgcttcgttt ccaagtcaat ccgcccgcag cgtccctcag gatttcggcg gagagccaag
     1021 tcgtccggga cgtatcgggc aacgatggag cggagcaccc tagccagagc ctcccggtcg
     1081 tatatccggc aaggtaatcg tctccggctg gcgtgctccc cggcccgagg ccgagcagat
     1141 cgccgaagcg gcggtcccga gccggagcag cgagaggctc cagctcctct tcgagcagcg
     1201 aacgccactc gcgaaccaac ggcgcccatc gaagattcat acgtccggcg ggaagcgacg
     1261 ggtcgtaccg cgcagggatt ccctcccccg gtagagagag aagctctccc tgccgcagag
     1321 ggggaagaag gtccgtcagc ca
//
LOCUS       DAOSWW010000854         1342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91112_length_1342_cov_2.127428, whole genome shotgun sequence.
ACCESSION   DAOSWW010000854 DAOSWW010000000
VERSION     DAOSWW010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91112_length_1342_cov_2.127428"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PK442_13660"
     CDS             complement(<1..1297)
                     /locus_tag="PK442_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88745.1"
                     /translation="MLAHRLRALLMNEVSSDSVSEFYSYRYLASEGFLPGYNFTRLPI
                     RLFLDVGKGTDAISRDRVLAIREMGPENLVYHNGSKYKVTRAQIRETADETQEATVCL
                     DSGYILFDGEQGLNTDPWSGAGLDGRTLRIAGLLPLPDALAERTKHITCDEEERQRIG
                     YQIDTYFRFKGDEKRLSEIRLVAGEDVLLRLRFIPSAELIYVNSKWRIAKEEGFVLNR
                     VSGHWKSHDFRERLLSGQEKGTKMKAEDLKVVKLYTGDTADALYIEPLKILDLDRSGR
                     VTLQYALKTAVERVFQVESAELGVTPIGNPDSPNLLMFEASEGSLGVMAAMVRESSAW
                     RRVVDEAWKICRYDEEDYKEKASYDDLLSYYNQPDHAAIDRHLIKGALERLRDAREEV
                     GSSEAGTYEEQYRRLLEKHDENSSTERRLLDFLHERGLRL"
BASE COUNT          211 a          398 c          432 g          301 t
ORIGIN      
        1 ggaggcgaag ccctctttcg tggagaaagt cgagtaagcg ccgctccgtg gagctgttct
       61 catcgtgctt ctccagtaag cgccggtact gctcttcata ggttcccgct tcggaggagc
      121 cgacctcctc gcgggcgtcg cggagacgtt cgagcgcccc cttgataaga tgccggtcga
      181 tcgcggcgtg gtcgggctgg ttgtagtagc tcagcaggtc gtcgtagctg gccttctcct
      241 tgtagtcctc ctcgtcatag cggcagattt tccacgcctc gtcgacgacg cgcctccatg
      301 cggagctttc gcggaccata gccgccatga cgccgagact cccctccgac gcctcaaaca
      361 tgagcaggtt cggcgaatcg gggttgccga tgggggtgac gccgagttcc gcgctctcca
      421 cctggaagac acgttctacg gcggttttga gggcgtactg cagcgtgacg cgtccggatc
      481 tgtcgaggtc gaggatcttg agcggctcga tgtagagagc gtccgccgtg tcgcctgtgt
      541 agagcttgac gaccttcaga tcctccgcct tcatcttggt tcccttttcc tgtccgctca
      601 gcaggcgttc gcggaagtcg tggctcttcc agtggccgga gacgcggttg agcacgaacc
      661 cttcctcttt cgcgatccgc catttggagt tgacgtagat cagctcggcg gagggtatga
      721 agcggaggcg aagcagaacg tcctctccgg cgacgaggcg gatttcggag aggcgcttct
      781 cgtccccctt gaagcggaag taggtgtcga tctggtagcc tatccgctgc cgctcctcct
      841 cgtcgcaggt gatgtgtttg gtgcgttccg caagggcgtc cggcagagga agaagccccg
      901 ctatccggag agtgcgtccg tcgagtccgg cgccgctcca cgggtcggtg ttcagccctt
      961 gctcgccgtc gaagaggatg tagccggaat ccagacagac ggtcgcttcc tgcgtttcgt
     1021 ccgccgtctc gcgtatctgg gcgcgggtga ccttgtactt cgatccgttg tggtagacga
     1081 ggttttccgg tcccatctcg cggatggcga gcacacgatc ccggctgatc gcgtcggttc
     1141 cttttccgac gtcgaggaag agacggatcg ggaggcgggt gaagttgtat ccgggcagaa
     1201 atccttcgct cgcgagatag cggtagctgt agaactcgga gaccgaatcc gatgacactt
     1261 cgttcatcag gagagcccgc aggcggtggg cgagcatttc gttccgctgc gcctccctgt
     1321 attccgggct cgtcatcttg aa
//
LOCUS       DAOSWW010000855         1340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91328_length_1340_cov_2.170428, whole genome shotgun sequence.
ACCESSION   DAOSWW010000855 DAOSWW010000000
VERSION     DAOSWW010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91328_length_1340_cov_2.170428"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PK442_13665"
     CDS             complement(<1..911)
                     /locus_tag="PK442_13665"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOO88746.1"
                     /translation="MFEQTFRNIDDLLRKEAGCTTELDYTEQSSWLLFLKYLEGLEQD
                     RADEALLSGKPYAYILDAPYRWESWAAPKGKDGQLDRNRAMTGDDLRDFVNQKLFPYL
                     HGFKSRATGPNTVEYKIGEIFGEIKNKIQSGYNLREIIDYVDELKFRSQVEKHELSAL
                     YEEKIQRMGNAGRNGGEYYTPRPLIRAIVEVVDPKIGERVYDGACGSAGFLCEAHVHM
                     KKRNPRRTTAQDISLMKYVGMNPVVVHGGGPAISRTLKDLSIQSRFHNGLRVTDADTI
                     GVVEMVLAGKVNKDIVSLLNQAGGQAVG"
     gene            complement(1087..>1340)
                     /locus_tag="PK442_13670"
     CDS             complement(1087..>1340)
                     /locus_tag="PK442_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88747.1"
                     /translation="VQEKKDVAQRSLCRCAPRVDIGAYDKCPAGCANYSATQSTAAVR
                     ANVSRHDPASPLLTGTPSPEEQALIERSRTQQQISLGLL"
BASE COUNT          240 a          388 c          399 g          313 t
ORIGIN      
        1 cccacggcct ggcccccggc ctgattgagc aggctgacga tatccttatt cactttgccg
       61 gccagcacca tctccacaac gccgatggta tcggcatcgg tcacgcgcag accgttgtgg
      121 aagcgcgact ggatggacag gtccttcagc gttctgctga tggccggacc gccaccgtgc
      181 acgaccacgg ggttcatacc gacgtacttc atcaaggaga tgtcctgggc cgtggtgcgg
      241 cgcggattgc gcttcttcat atgaacgtgc gcttcgcaca ggaaacctgc ggaaccgcag
      301 gctccgtcat agacgcgttc tccgatcttg gggtcgacta cttcgacgat ggcgcggatc
      361 agcggacgcg gcgtgtagta ctcgccgccg ttgcgaccgg cattccccat gcgctggatc
      421 ttttcttcgt acagcgccga gagttcgtgc ttttccactt gggagcggaa cttcaattcg
      481 tcgacgtaat cgatgatctc tctgagattg tagccgctct gaattttgtt cttgatctcg
      541 ccgaagatct cgccgatctt gtactccacc gtgttgggac cggtggcgcg gcttttaaat
      601 ccgtgcagat agggaaagag cttctgattc acgaagtctc gcagatcgtc tccggtcatg
      661 gcccggttgc ggtcgagctg cccgtccttc cctttcgggg ccgcccacga ttcccagcga
      721 tagggggcgt cgagaatata tgcgtacggc ttcccggaca agagggcctc gtccgccctg
      781 tcctgttcca gcccctccag gtatttcaaa aacaggagcc acgaagactg ctccgtgtag
      841 tcgagttccg tagtgcaccc cgcctctttt ctgaggaggt cgtcgatatt ccggaaggtc
      901 tgctcgaaca tgcgtttgcc tcttcctttc atgtgtgaag accgccggga atgttccgcc
      961 gatcgggtgt catttattcc gtaatgtcat tagcgtcacg atagcattga tgagaagggt
     1021 tgtcaatttt gctccgtcaa gaagcaaggg gcgagccgat gactagaggg cgctgccgcc
     1081 ggtgtattac aaaaggccga gagatatctg ctgctgtgtg cgcgaccgct ctatgagcgc
     1141 ctgctcttcg ggcgacgggg ttccggtcaa caggggagag gcggggtcat ggcgcgagac
     1201 atttgcacgg acggccgccg tgctttgtgt ggcagagtag ttcgcgcacc cggccgggca
     1261 cttgtcgtag gcgccgatgt ccactctcgg cgcgcaacga caaagggaac gctgtgctac
     1321 atcctttttc tcctgaacgg
//
LOCUS       DAOSWW010000856         1339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91451_length_1339_cov_1.848131, whole genome shotgun sequence.
ACCESSION   DAOSWW010000856 DAOSWW010000000
VERSION     DAOSWW010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91451_length_1339_cov_1.848131"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1339
                     /locus_tag="PK442_13675"
     CDS             <1..>1339
                     /locus_tag="PK442_13675"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOO88748.1"
                     /translation="TRDELEDGNSFDSKALPEEYLTNPRETHTFIYVSTIQRMAINLL
                     GKDAAGGFEYDVDANKLDIPINAFDVIIADECHRGYSAKETGCWKYVLDYFDAVKIGL
                     TATPAAHTLAMFKNKAYSYSTEQAILDGYLVDYDAVKIASDIHINGAFLKEGELVGEI
                     DVETGAEQLDALEDEREFGASEIERNLTAPDSTKKIIAALKKYTDRHEAEYARFPKTL
                     IFAVNDIPHVSHADEVVRTCKEIFGKGDDFVLKITGSPTVDRPLQKIRQFRNRPEPKI
                     VVTVDMLTTGVDIPAIEMIVFMRMVKSRILWVQMLGRGTRLCKEINKEKFTIFDCFDG
                     SLVEYFKNATDFDVNLQKETVPLAKIIERIYDNRDREYNSNRLIKRLRRIENAMGAEA
                     REAFARFIPDGDMKAYADGLKENILNHFTETMTLLRNKEFQELWSMCTLIPVKR"
BASE COUNT          343 a          454 c          339 g          203 t
ORIGIN      
        1 cacgcgcgac gagctggaag acggaaacag cttcgactcc aaagcgctcc ccgaagagta
       61 cctcaccaac ccccgggaga cgcacacctt catctacgtc tccaccattc agcgcatggc
      121 gatcaacctg ctcggcaaag acgccgccgg cggtttcgag tacgacgtcg acgccaacaa
      181 gctcgacatc cccatcaacg ccttcgacgt catcatcgcc gacgagtgcc accgcggcta
      241 ctccgccaaa gaaaccggct gctggaaata cgtcctcgac tacttcgacg ccgtgaaaat
      301 cggcctcacc gccacgcccg ccgcgcatac cctcgccatg ttcaaaaaca aggcgtactc
      361 ctacagcacc gaacaggcca tcctcgacgg ctacctcgtg gactacgacg ccgtcaagat
      421 cgcctccgac atccacatca acggcgcgtt cctcaaagaa ggggagctgg tcggcgaaat
      481 cgacgtggag accggggccg agcagctcga cgcgctggaa gacgaaagag agttcggcgc
      541 gtcggaaata gagcggaatc tcaccgctcc cgacagcacc aaaaagatca tcgccgccct
      601 caaaaagtac accgaccggc acgaggccga gtatgcccgc ttccccaaga ccctgatctt
      661 cgcggtcaac gacattcccc atgtctcgca cgccgacgaa gtggtgcgca cctgcaagga
      721 aatcttcggc aaaggcgacg acttcgtgct gaaaatcacc ggaagcccca ccgtcgaccg
      781 ccccctgcag aaaatacgcc agttccgcaa ccgccccgag cccaaaatag tcgtcaccgt
      841 ggacatgctg accaccggcg tggatatccc cgccatagaa atgatcgttt tcatgcggat
      901 ggtgaagtcc cgcatcctct gggtgcagat gcttgggcgc ggaacccggc tgtgcaagga
      961 gatcaacaaa gagaagttca ccatcttcga ctgcttcgac ggcagcctcg tcgaatactt
     1021 caagaacgcc accgacttcg acgtcaacct ccagaaagag acggtcccgc tcgccaaaat
     1081 catcgaacga atctacgaca accgcgaccg ggagtataat agcaaccgtc tgatcaaacg
     1141 cctgcggcgc atcgaaaacg ccatgggcgc ggaagcgcgc gaagccttcg cccgattcat
     1201 ccccgacggc gacatgaaag cctacgccga cgggctgaaa gagaacatcc tgaaccactt
     1261 caccgaaacg atgacgcttc tcaggaacaa agagttccag gagctgtggt caatgtgcac
     1321 cctcatcccg gtgaagcgg
//
LOCUS       DAOSWW010000857         1338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91501_length_1338_cov_3.118472, whole genome shotgun sequence.
ACCESSION   DAOSWW010000857 DAOSWW010000000
VERSION     DAOSWW010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91501_length_1338_cov_3.118472"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK442_13680"
     CDS             <1..313
                     /locus_tag="PK442_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO88749.1"
                     /translation="LSGGNAQKVVVAREVSRKAEVLLASQPTRGVDIGSIGTIRNELN
                     RAKEAGAAVVLISSDLEEILSLSDRIAVMFEGRITGEVDSSEADEEKLGLLMTGGGTH
                     V"
     gene            306..>1338
                     /locus_tag="PK442_13685"
     CDS             306..>1338
                     /locus_tag="PK442_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO88750.1"
                     /translation="MYKTSWGSTLMTIILSLALGAGAILLLGYDPIEAYYQLFRGAFV
                     GKFNIGGVMERFVPLMLTALAFAVSAKVAVFNVGVEGELYLGAIAAAWAGYAFTGLHP
                     LVHIPLCFAVAMVASGAWGAIPGALKAYWKVNEVCVTILMNYVAIYFTSYLVGNPFSA
                     RASIPRTPTIEESARLLRILRPSRANIGIFVALGVLVLVWWMLYHTNWGYKLRHVGQN
                     PSFSEYVGIPPKKAMIWGMILSGAIGGLAGAIEVMGIHGHFLDNFSPGIAFDGMLASL
                     IAKNDIRMVPVLSLFLAVLKTGALGMERFTGVPKTLIDTIIAVFILLAAMEGLFAYSR
                     RRTARSSGEE"
BASE COUNT          226 a          421 c          419 g          272 t
ORIGIN      
        1 gctctccggc gggaacgccc aaaaggtcgt cgtagcgcgg gaggtctccc ggaaagccga
       61 ggtgctgctg gcgtcgcagc ccacgcgggg cgtcgatatc ggctcgatcg gaaccatacg
      121 gaacgagctg aaccgcgcca aagaagcggg ggcggcggtt gtgctgatct cttccgatct
      181 cgaggagatc ctctccctct ccgacaggat cgccgtcatg ttcgagggac ggatcacagg
      241 agaggtcgat tcctcggagg ccgacgagga gaagctcggc ctgctgatga ctggaggcgg
      301 gacgcatgta taagacatcc tggggcagca cgctgatgac catcatcctc tcccttgcct
      361 taggagcggg ggccatcctg ctgctcggct acgatcccat cgaagcctac taccagctct
      421 tccgcggggc cttcgtggga aaattcaaca tcggcggcgt catggaacgt ttcgtcccgc
      481 tgatgcttac ggcccttgcc ttcgcggtct ccgcaaaggt cgccgtgttc aacgtcggcg
      541 tcgagggaga gctctacctg ggcgccatcg ccgcggcgtg ggcggggtac gctttcaccg
      601 ggcttcatcc tcttgtccat attcctctct gcttcgccgt cgcaatggtc gcttccggag
      661 cgtggggggc tatccccggc gcgctgaagg cgtactggaa ggtgaacgag gtctgcgtca
      721 ccatcctgat gaactacgtc gccatctact tcacctccta cctggtgggg aaccccttct
      781 ccgcgcgcgc gagcatcccc agaacgccca ccatcgaaga gtcggcgcgt ctgcttcgga
      841 ttctccgtcc cagccgggcg aacatcggta tcttcgtcgc gctcggcgtg ctcgtgctgg
      901 tctggtggat gctctatcat accaactggg ggtacaagct gcgccacgtc gggcagaacc
      961 catctttctc ggagtacgtc ggtattcctc cgaagaaggc gatgatctgg gggatgattc
     1021 tctccggagc cattggcggt ctcgcggggg ccatcgaggt gatgggcatc cacgggcact
     1081 tcctcgacaa cttctcgccg ggaatcgcct ttgacgggat gctcgcctcg ctgatcgcga
     1141 agaacgacat ccgcatggtt cccgtactct cgctcttcct ggccgtcctc aagacagggg
     1201 cgctgggaat ggagcgcttc acaggagtcc ccaagaccct gatcgacacg atcatcgcgg
     1261 tcttcatcct gcttgcggcg atggaggggc tcttcgccta ttcgcggcgc agaaccgccc
     1321 ggagttccgg agaggagg
//
LOCUS       DAOSWW010000858         1338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91549_length_1338_cov_2.281372, whole genome shotgun sequence.
ACCESSION   DAOSWW010000858 DAOSWW010000000
VERSION     DAOSWW010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91549_length_1338_cov_2.281372"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..>1338)
                     /locus_tag="PK442_13690"
     CDS             complement(403..>1338)
                     /locus_tag="PK442_13690"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HOO88751.1"
                     /translation="NAVSYASSLAMDNFKVRFYGKASHAASAPWAGRSALDAVELMNI
                     GMNYMREHVRPESRIMYSIPDGGKAPNVVPPFAEVWYFVRAPQYKTVKEIMDWTKKVA
                     EGAALMTQTRMEFIPITAVWQYLPNQALAKVGFANAQLIGIPPFTDEDEKIAEPFSKS
                     VKVEKGPFLSREFTQYDYDKPFSWATGGGSIDEANTSWVVPMVRFSGSTLAQGTPGHS
                     WQATAQNALPPAFKAALTVAKYMAATALDLYANPSLLEEALAELKASNEKHGPFVDPA
                     KDVPLPTFQLMHNVDESQVPVQTKDMPMPDFSTLK"
BASE COUNT          254 a          362 c          417 g          305 t
ORIGIN      
        1 ccgtgcgccc tcgaatattc gctttttacc gcagccggtt gctccgcggc gagactcaac
       61 ggaagacagg gaaaaacgtt ccatgacgcg gtagaggtcg aagctgttct cctcgttgag
      121 gaatccgtcg ccttcattct ccttgaccag gtactccacg tcaatacatc gttgcctcaa
      181 ggcttccgca atcgtatccg atccggcctt tttcacgcgg ggggatgttg gctccccggt
      241 aacgacgagc agcgcccaag agtaacgacc ggctccgcaa ggttcgccag tttgccgaac
      301 ctttgcgttt tccggtcgaa gaagcggcag tgcacacggt ctgttatgca gcgaaatgcg
      361 tgccggagga aaagctccct ccggcacatg gtgtcaacag acttatttca aagtggaaaa
      421 atcgggcatg ggcatgtctt ttgtctgaac gggtacctgc gactcgtcaa cattgtgcat
      481 gagctggaat gtgggaaggg gtacgtcttt tgccggatca acaaaggggc cgtgtttctc
      541 gttggaggct ttcagctcgg ccagcgcctc ctcaagaagc gaggggtttg cataaaggtc
      601 gagggcggtc gcggccatgt atttcgccac ggtaagagct gccttgaacg ctgggggaag
      661 cgcgttctgc gctgtggcct gccaggagtg ccccggagtt ccctgggcga gagtcgagcc
      721 gctgaagcga accatcggca ccacccagct tgtattggcc tcatcgatgg agccacctcc
      781 ggttgcccag gagaatggct tgtcgtagtc gtattgggtg aactctctgg agaggaaggg
      841 gcctttctcc accttgacgc tctttgagaa gggttcggct atcttctcat cttcgtcggt
      901 gaacgggggg atgccgatga gctgcgcgtt ggcgaatccg accttcgcca gcgcctggtt
      961 gggcagatac tgccagacgg cggtgatggg aatgaactcc atcctggttt gcgtcatgag
     1021 ggcggcgcct tcagctactt tcttcgtcca gtccatgatc tccttgacgg tcttatactg
     1081 gggggcgcgg acgaagtacc agacttcagc gaacggaggt acgacgttag gagccttccc
     1141 gccgtcggga atggagtaca tgattctcga ctcgggacgg acgtgctccc gcatgtagtt
     1201 catgccgatg ttcatcagtt cgaccgcgtc gagcgcgctt ctgcccgccc atggggcgga
     1261 ggctgcgtgc gaagcttttc cgtagaagcg gaccttgaag ttgtccattg cgagggagga
     1321 tgcgtacgaa acggcgtt
//
LOCUS       DAOSWW010000859         1336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91717_length_1336_cov_4.444965, whole genome shotgun sequence.
ACCESSION   DAOSWW010000859 DAOSWW010000000
VERSION     DAOSWW010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91717_length_1336_cov_4.444965"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..675
                     /locus_tag="PK442_13695"
     CDS             337..675
                     /locus_tag="PK442_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HOO88752.1"
                     /translation="MARSNVATEIKKAEEEAKVLVQDARSEARKIVASAKNQAEEMEK
                     TARQEARMHYRKSIQQAEIQAEDAAKKILEKGQKEAKVFVSENASRVGAVANWIAEEV
                     MARYDSSRSK"
     gene            complement(968..>1336)
                     /locus_tag="PK442_13700"
     CDS             complement(968..>1336)
                     /locus_tag="PK442_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88753.1"
                     /translation="FLGQIHTDETELDENFRGRFPVFPRKKSHKKFFVLRISIIVSVG
                     LGIGGNASKPLLRLGYVIPGKGSQNAPNVFGAPGNKGKFMRKREKRVQNGLKKLAPGI
                     LTTILRKEPLFLAQSLHQGL"
BASE COUNT          325 a          299 c          372 g          340 t
ORIGIN      
        1 cagttggtag agcatcagct tcccaagctg agggtcgcgg gttcgagtcc cgttttccgc
       61 tccacgagtg tgacgggaag ggcttttgcc tttccctttt tttgtagcct tgcaaatttt
      121 acaaaattgc agtaagagcc tacttgtatg catttttgga aaattttttg ctctttcggc
      181 tgtcaaaaaa cacttttttg gcctttttgg aggaagaggg gaaatccgtt ttgcgatatt
      241 tttctcaagc ggaggcgatc ttttgtttgc cataactgaa atatggtttt acaattgtca
      301 aggttccata ttattgaccg gaaggaggtg acagcgttgg cgcgctcgaa tgtagctacg
      361 gagatcaaaa aagctgaaga agaagcaaag gttctcgttc aggatgcccg gagtgaagcc
      421 cgtaaaattg tggcctccgc taaaaaccag gcggaggaaa tggagaaaac cgcccgacag
      481 gaagctcgga tgcactacag aaaaagcatt cagcaggcgg aaattcaggc ggaagacgca
      541 gcaaagaaaa ttcttgaaaa gggtcagaag gaggcgaaag tcttcgtctc cgaaaatgca
      601 tcgcgtgtag gagctgtcgc aaactggata gcggaagagg tgatggcgcg atatgacagt
      661 agccgcagta agtaagatcg gactggcggt tcacaaggaa tcggaacgag cggttcttgc
      721 ggcccttcag tctttcgggc tctgcgagat tgtcgctccc cgggaggagg acgcggaaaa
      781 tcgggatcaa cggcagaaaa cctttgccta tggtttcgat gtggatgcct cgtcggggga
      841 tgcccgttac gctttgcggg ttttggaacc ctactatcag gattcggggg atcttctgag
      901 cagaatgctg gagctccccg aaagaaattc cgttgcacaa ctccgccgga catatgaaga
      961 ttttcctcta caatccctgg tggagactct gcgccaaaaa gagaggctcc ttgcggagaa
     1021 tcgtagtgag tattcccgga gcgagctttt tcagtccgtt ttggactctc ttctctcttt
     1081 tcctcatgaa ctttccctta ttacccgggg caccgaatac gttcggggcg ttttgggaac
     1141 ccttcccggg gataacgtag ccaaggcgga ggaggggctt cgaagcgttt cctcctattc
     1201 cgaggcctac gcttacgatt atggagattc taagaacgaa aaactttttg tggctctttt
     1261 tcctcgggaa aacggggaat ctgccacgga agttctcatc aagctcggtt tcgtccgtat
     1321 ggatttgccc caggaa
//
LOCUS       DAOSWW010000860         1336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91727_length_1336_cov_3.693989, whole genome shotgun sequence.
ACCESSION   DAOSWW010000860 DAOSWW010000000
VERSION     DAOSWW010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91727_length_1336_cov_3.693989"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PK442_13705"
     CDS             complement(<1..417)
                     /locus_tag="PK442_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO88754.1"
                     /translation="MEHILEEAKRISAEITEWRRHLHQTPELGLETPKTSAYIVQELK
                     KMGVEEIRERVGGWGVAALVKGEKPGKTLAIRADCDALPIKEETGLPFASKNGLMHAC
                     GHDAHTAMALGAAKLLLAHKAELAGTVKFIFQPAEEN"
     gene            764..>1336
                     /locus_tag="PK442_13710"
     CDS             764..>1336
                     /locus_tag="PK442_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88755.1"
                     /translation="MMHIFFVHKKALIACLTILSLLALMPSTGAASAPAPNTFLSSPL
                     YGITHFDSSQSDSTPYGPPRGTFYIESSLQPISYGGPVNIITLASTDPEYMWAVGHDK
                     VSYVHRVSGEWKVVASFEALADASNGLLPAVPDEKFREFGMRNAIGMTVAQMDTYLTE
                     ILGENYKARFGNGAYSVVDKDNVLYTNFLSS"
BASE COUNT          242 a          417 c          356 g          321 t
ORIGIN      
        1 gttctcctcg gcgggctgga agatgaactt cacagtgccg gcaagctcgg ccttgtgcgc
       61 cagcagcagc ttcgccgcgc ccagcgccat cgcggtgtgc gcgtcgtgcc cgcaggcgtg
      121 catgaggccg ttcttggacg cgaacggtag tcccgtctcc tccttgatcg gcagcgcgtc
      181 gcagtcggcg cggatcgcca gcgtcttccc cggtttctct cccttcacga gcgccgcgac
      241 gccccatccg ccgacgcgtt cgcggatctc ctccactccc atcttcttca gttcctggac
      301 gatgtaggcc gacgtcttcg gcgtctccag cccaagctcc ggcgtctggt gcagatgccg
      361 ccgccactcc gtgatctccg cggagatcct ctttgcttct tcaagaatgt gctccattcc
      421 tccccctccg ttcgtgcagg atgattttcg ctccattcta catcattgag attcttttct
      481 cttctgtcgg aagagcgtgc atcactcgct ctttttgcat ccggtaaaat gtgcgataat
      541 ctggtgtatc tactcccttt cgtgcagcga acccgttttc acccgtacgg aaggggttct
      601 tcgacgaaag gagggtgcga acgggcgcgg tgtttttgaa aagcgcacag tgccggctct
      661 ctgaatccgc ttcttcggaa cgttttcggg aggatctgcg tcgcttcgga tgaatgaacc
      721 cgggatactc gatgttcatc agaatttatt atcaggaggt tcgatgatgc acatattttt
      781 tgttcataag aaagcattga ttgcgtgcct gacaatactc tctcttctcg cgctcatgcc
      841 ttcaaccggg gcggcgagcg cccccgcccc caatacgttt ctgtcgtcgc ccctgtacgg
      901 cattactcat ttcgattctt cccagagcga ctccacgccc tacggccctc cccgcggcac
      961 gttctacatc gagtcgtcgt tacagcccat ctcttacggc ggaccggtga acatcattac
     1021 gctcgcgtcc accgatccgg agtacatgtg ggctgtcggg cacgacaaag tgagctacgt
     1081 ccacagggtg agcggagaat ggaaggtcgt cgcctcgttc gaggcccttg ccgacgcgtc
     1141 gaacggcctc ttgcccgcag ttccggacga gaaattcagg gaattcggca tgaggaacgc
     1201 aatcggaatg accgtggccc aaatggacac ataccttacg gaaattcttg gggagaacta
     1261 caaggcgcgc ttcggtaacg gagcctactc ggttgtcgac aaggacaacg tactctacac
     1321 gaacttcttg tccagc
//
LOCUS       DAOSWW010000861         1335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91834_length_1335_cov_3.437500, whole genome shotgun sequence.
ACCESSION   DAOSWW010000861 DAOSWW010000000
VERSION     DAOSWW010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91834_length_1335_cov_3.437500"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PK442_13715"
     CDS             <1..469
                     /locus_tag="PK442_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO88756.1"
                     /translation="GRNACLSSTYGTEARGTFTKSDVLIGDGEIDFIEVVEPDVLLCL
                     AQTAFDRSTPRLEAEGAERTLLLYDSDLVTPSPLCRAKSIGIPFARLASEQGAPGAAN
                     LAALGAVVALTGAVRPESVRAILEERAFRQGSAEAFDEGLRAAKTGEWIPPRN"
     gene            653..>1335
                     /locus_tag="PK442_13720"
     CDS             653..>1335
                     /locus_tag="PK442_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOO88757.1"
                     /translation="MQQQHAERVYFFDAYRTIIVLSVLALHAAMSYMAYVPGWWYVIN
                     PEKSLFFLFWVLIVNTGDMPAIFFLAGYFAPRSLEKRGRMLFLKEKALHIGIPWIVGV
                     LAFAPLFAMATWRSLNLPLPETYMEFVRTIWLGPGYQQSHFWFLGVLMVFLIVFAAVG
                     SPRASSGRSPLIWLAGWWAVSSAAFFLSSLHWHPDLWIRVSHIYFQPARIVSYVLAFY
                     LGARAWRDG"
BASE COUNT          209 a          436 c          424 g          266 t
ORIGIN      
        1 agggcggaac gcctgcctct cctcgaccta cggcaccgag gcgcggggga cgtttacgaa
       61 gtcggacgtc ctcatcggcg acggcgagat cgacttcatc gaggtcgtcg agccggacgt
      121 gctgctctgc ctggcgcaga cggccttcga tcgctccacc ccccggctgg aggcggaggg
      181 ggccgaacga acgctgctgc tctacgacag cgatctggtg acgccttcgc cgctctgccg
      241 ggcgaaaagc atcggcatcc ccttcgcacg cctcgcgtcg gagcagggcg ctccgggcgc
      301 ggcgaacctc gcggcgctgg gggccgtcgt cgcgctgacg ggcgcagtgc gtccggagtc
      361 ggtgcgcgca atcctcgaag aaagggcgtt caggcagggg agcgcggagg cattcgatga
      421 agggctccgc gctgcgaaaa ccggagagtg gattcctccg aggaattagc ccggcgggga
      481 ggcggattcc gcgtttttat ccgtcgagca tctccccgga acggcagcaa ggacactgaa
      541 tggaaaccgg atgcgcacgg acggaatcct ttcgcggcgc ggttttttcc ggcgtcccgt
      601 tgtgcagaac aatgaggata cagttcatca aaaaaaagcg aggtgggatt tcatgcaaca
      661 gcaacacgcg gagcgggtct acttcttcga cgcctaccgg acgatcatcg tcctctccgt
      721 cctggcgctt cacgcggcga tgagctatat ggcctacgtt ccggggtggt ggtacgtcat
      781 caaccccgag aagagcctct tcttcctctt ctgggtgctc atcgtgaaca cgggcgacat
      841 gcccgcgatc ttcttcctgg cggggtactt cgccccccgt tcgctcgaaa agcggggacg
      901 gatgctcttt ctgaaggaaa aagcgctcca catcggcatt ccatggattg tcggcgtcct
      961 cgcgttcgcg ccgcttttcg ccatggcgac gtggcgctcg ctgaatctgc cgctgccgga
     1021 gacgtacatg gagttcgtcc ggacgatatg gctcgggccg ggctatcagc agtctcactt
     1081 ctggttcctc ggcgtgctca tggtcttcct gatcgtcttc gccgccgtcg gctcgccgcg
     1141 cgcctcctcc gggcgctcgc ccctcatctg gctcgctggg tggtgggccg tctcatccgc
     1201 cgcgttcttc ctttcgtcgc tccactggca ccctgacctc tggatacgag tcagccacat
     1261 ctacttccaa cccgcccgga tcgtctccta cgtcctcgcc ttctacctgg gagcgcgggc
     1321 gtggcgcgac ggctg
//
LOCUS       DAOSWW010000862         1334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_91948_length_1334_cov_3.454261, whole genome shotgun sequence.
ACCESSION   DAOSWW010000862 DAOSWW010000000
VERSION     DAOSWW010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91948_length_1334_cov_3.454261"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PK442_13725"
     CDS             complement(<1..1172)
                     /locus_tag="PK442_13725"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOO88758.1"
                     /translation="MAKYVFVTGGVVSSLGKGITAASLGVLLKKRGLRVSIIKLDPYL
                     NVDAGTMNPFQHGEVFVTDDGAETDLDLGHYERFIDESLSANNNVTTGKIYSSVIAKE
                     RRGDYLGGTVQVIPHITNEIQERILRAGEKSDIVIAEIGGTVGDIEGQPFLEAIRQMS
                     SRVGRENVLYCHVTLVPWLEAARELKTKPTQHSVQELRRIGILPQILVCRSHYAIEEE
                     MRDKMALFCNVPPEAVIQALDEPTIYKVPLSLYEQKFDLLVLKGLSLESPREPDLSDW
                     RRVVDGFCSPAGAVDIVMVGKYINHKDAYLSVVEALHHAGIHHDVKVNIRSVEAEEIE
                     EHGSEKILSGAHGILVPGGFGSRGIEGKIAAARFARERKIPYLGLCLGMQVAVVEFA"
BASE COUNT          306 a          413 c          328 g          287 t
ORIGIN      
        1 gcaaactcca ccaccgcaac ctgcattccc agacagagac cgagataggg aattttccgc
       61 tcacgggcaa agcgagccgc agcgatcttt ccctcgattc cgcgcgaacc gaaaccgccg
      121 gggaccagaa taccatgcgc gcctgaaagg atcttctcgc ttccatgctc ttcgatctct
      181 tctgcttcca ctgaacggat attcaccttc acgtcatggt gaatcccggc gtgatggagc
      241 gcttccacca cgctcaggta ggcgtccttg tgattgatat atttccccac catgacaata
      301 tcgactgctc ccgcggggga gcagaatccg tcgacaactc ttctccagtc ggaaagatcc
      361 ggttcgcggg gagattcaag agaaaggccc ttgagtacga gcaagtcgaa cttttgttca
      421 tacaggctga gaggcacctt gtagatcgtc ggttcgtcaa gagcctgaat gactgcttcc
      481 ggcgggacgt tgcagaagag cgccatcttg tcgcgcatct cttcctcgat agcgtaatgg
      541 gaacggcaca ccaggatttg agggaggata ccaatacgcc gcagctcctg aacgctatgc
      601 tgcgtcggtt tggtcttcaa ttcgcgcgcc gcctcaagcc agggcacaag cgtcacgtga
      661 caatagagca cattctcgcg tcctaccctg gacgacatct gacggatggc ctccaggaag
      721 ggctgtcctt caatatcgcc cacggttccg ccgatctccg cgatgacaat gtcgctcttc
      781 tcgcccgccc ggaggatacg ctcctgaatc tcgttggtga tgtgcggaat gacctgcacc
      841 gtccccccca gataatcgcc gcggcgctcc ttggcgatga cggaagagta gatcttcccc
      901 gttgtcacat tgttgttcgc gctcagcgac tcatcgataa accgttcgta atgtcccaga
      961 tccaggtcgg tttcggcgcc gtcgtcggtc acgaaaacct ctccgtgctg gaagggattc
     1021 atcgtccccg cgtccacgtt caaatacgga tcaagtttga tgatggagac gcgcagcccc
     1081 ctctttttga gcaacacgcc cagcgacgcc gcagtgatac cctttcccaa cgacgacacc
     1141 actccgcctg tgacaaacac gtacttcgcc atttccctca tcctccacta gtattcagaa
     1201 aaaatcgttc acgagacgaa cacgtcaacg catcaaaaaa acatggacca gggagaaccc
     1261 cggtccagaa tatcgaaaga gaatccatcc gtcatggagg atattgccta tcttaacatc
     1321 cccagcgggt tcac
//
LOCUS       DAOSWW010000863         1330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_92372_length_1330_cov_3.268235, whole genome shotgun sequence.
ACCESSION   DAOSWW010000863 DAOSWW010000000
VERSION     DAOSWW010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92372_length_1330_cov_3.268235"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1330
                     /locus_tag="PK442_13730"
     CDS             <1..>1330
                     /locus_tag="PK442_13730"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO88759.1"
                     /translation="DKYEQSTQTESIIQSYGDQLRSVVDIAENIVRQIMKSAEEGQFS
                     REEAKRQAMIALRALRFDDGLGYVWVNDTSLPYPSFLMHPTQPELEGVLMKGPDFDKA
                     MGSEKNIGKSILEACALDGEGFVQYLWSKPTRDGLIPDIPKLSYVRSIKEWGWIIGTG
                     VYVDDAREYAVRQALDIIANMRYAGGAGYFWVTDTHRPNPRPIIHPTLDDAENGLPGI
                     TPGDESYDTARKFVEEVGVQAADVCEANGEGYVQYLWPRIGGNGTLELAPKETFVRLF
                     EPLQWIIGTGVHLDDVDRAVALRQAESKRQRDIVLRGTLFAALLLVALAFVLLYFVVR
                     GIVRPIVQLTAAADMVASGDMETPLPDSGPSEIRSLSRSFNRMTEELKERMTALRESE
                     GRFRRLAENSPDMIFRISLRDGRYEYISPAVTRLLGYSQEEAYATPFFARK"
BASE COUNT          281 a          408 c          402 g          239 t
ORIGIN      
        1 cgacaagtac gaacagtcga cgcagaccga atctatcatc cagtcgtacg gagaccagct
       61 ccgctcggtg gtcgacatcg cggaaaacat cgtccggcag atcatgaagt cggcggaaga
      121 ggggcaattt tcccgtgaag aggcaaaacg gcaggctatg attgcccttc gggccctgcg
      181 tttcgacgac ggcctcggct acgtctgggt caacgacaca tctctcccct acccttcgtt
      241 tcttatgcac cctacccaac ccgaactgga aggcgtcttg atgaagggcc cggacttcga
      301 taaagcgatg ggcagcgaaa agaacatagg caaatcgata ctcgaagcgt gcgcgctgga
      361 cggagaggga tttgtgcaat acctgtggtc caaacctacc agggacgggc tgattcctga
      421 tataccaaaa ctctcctacg tcagatcgat aaaggagtgg gggtggatca tcggcaccgg
      481 ggtctacgtg gacgacgccc gggagtacgc cgtccggcag gcgctggata tcatcgcaaa
      541 catgcgttat gccggaggcg cagggtactt ctgggtcacc gacacgcacc ggcccaaccc
      601 gaggccgatc attcatccga ccctcgacga cgccgaaaat ggactgccgg ggattacgcc
      661 gggcgatgag tcctacgata ccgcacgcaa gtttgtcgaa gaggtcggag tccaagcggc
      721 cgacgtgtgc gaagcaaacg gcgaggggta cgtgcagtac ctgtggcccc gaataggcgg
      781 aaacgggact cttgaactcg cgccgaagga gaccttcgtg cgtctcttcg aaccgctcca
      841 atggatcatc gggacgggcg tgcatctgga cgacgtcgac agggccgtcg ctcttcgcca
      901 ggccgagagc aaacggcagc gagacattgt gcttcgcggc acgctcttcg ccgcgctgct
      961 gctcgtcgca ctcgccttcg ttctgctcta tttcgtggtg cggggcatcg tccggccgat
     1021 cgtacagctt accgccgccg ccgacatggt ggcctccggg gatatggaaa cgccgctccc
     1081 ggactccggt ccgagcgaga ttcgctcact ctcccgctcc ttcaaccgga tgacggaaga
     1141 gctgaaagag cggatgaccg ccctccgcga gagcgaggga cgcttccgca ggctggcgga
     1201 gaactctccg gacatgattt tcagaatttc gctgcgcgac ggccgttacg agtacatcag
     1261 cccggccgtc acccggctgc tcggctactc ccaggaggag gcctacgcca cgcccttttt
     1321 cgcacgcaaa
//
LOCUS       DAOSWW010000864         1330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_92447_length_1330_cov_1.892549, whole genome shotgun sequence.
ACCESSION   DAOSWW010000864 DAOSWW010000000
VERSION     DAOSWW010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92447_length_1330_cov_1.892549"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PK442_13735"
     CDS             complement(<1..565)
                     /locus_tag="PK442_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOO88760.1"
                     /translation="METLSFFDIPGLRTGHAQNTEAGTGCTAVICEQGAVAGVDVRGG
                     APGTRETDLLDPGNMVERIHAVVLAGGSAFGLDAASGVMNLLEERGVGFDVGVTRVPI
                     VCSAVLFDLVCGDWRVRPDKAMGRQAAENAFEPAFIPLRGSVGAGTGALVGKFHGLDF
                     AMRGGLGSFAYRTGPLIVGALVAVNCLG"
     gene            complement(672..>1330)
                     /locus_tag="PK442_13740"
     CDS             complement(672..>1330)
                     /locus_tag="PK442_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO88761.1"
                     /translation="IGVRFGSLMASVDRFVVTFKGKGGHGATPHLTVDPVTMTATAVL
                     ELQTILSRELSPLDSAVLTIGRIEGGRAFNIIAENCVIEGTVRTLNPATRAFVEERLR
                     AVVSRVAEGMRGVAEVEYESGPPALINNADFTRKFHAFAAELAGAEKVKEISEPTMGG
                     EDVAYFLEMVPGTFFVHGSCAPEKGQVYPHHNPKFDVDEETFPLGTALFAGFALSWQQ
                     "
BASE COUNT          228 a          454 c          412 g          236 t
ORIGIN      
        1 ccccgagaca gttgaccgca acgagcgcgc cgacgatcag cggccctgtc cggtaggcga
       61 aggacccgag ccctccgcgc atcgcgaagt cgagtccgtg gaacttgccg acgagcgctc
      121 cggttcccgc gcccaccgat cctcgaagag gaatgaacgc aggttcgaag gcgttttcgg
      181 cggcttgccg ccccatcgct ttgtcaggac gcacccgcca gtcgccgcag accagatcga
      241 agagcactgc ggagcagacg atggggaccc tggttacgcc gacgtcgaag cctactcccc
      301 tctcctccag aaggttcatc acacccgagg ccgcgtccag tccgaaggcg ctgcccccgg
      361 cgaggaccac cgcgtggatt cgctccacca tattgcccgg gtccagcagg tcggtctcgc
      421 gcgttcccgg agcgccgccc cggacgtcga cgcccgcgac ggccccctgt tcgcagatga
      481 cggcggtgca gcctgtacct gcttcggtat tctgcgcgtg tccggtccgc aggccgggaa
      541 tgtcaaaaaa ggacaaggtt tccatacgct ctccccttcc gttacgacat ggtgagcact
      601 ttgatcagcg cgatgagcac gcccgtcacg ctctcgccgc ggaacgcccc tccctgccgc
      661 cgttccgtga tctactgctg ccagctaagc gcgaatcctg cgaagagggc ggttcccagc
      721 gggaaggtct cctcgtcgac gtcgaatttc ggattatgat gcggatagac ctgtcccttt
      781 tccggagcgc aggagccgtg gacgaagaag gttccgggga ccatttcgag gaagtaggcc
      841 acatcttcgc cgcccatcgt cggctcgctg atctccttca ccttttccgc tccggcaagc
      901 tccgccgcga aggcgtggaa tttgcgtgtg aagtcggcat tgttgatgag agcgggagga
      961 ccagactcgt actccacttc cgccaccccg cgcatcccct cggcgacgcg ggaaacgact
     1021 gcgcggagcc gttcctcgac gaatgcgcgt gtggccggat tcagcgtccg gacggtgccc
     1081 tcgatgacgc agttctccgc gatgatgttg aacgccctgc ctccctcgat tcgtccgatg
     1141 gtgagcacgg ccgagtcgag cggagagagt tcgcggctca ggatcgtctg cagctccagc
     1201 accgcagtgg cggtcatcgt gacggggtcg acggtgaggt gcggcgtcgc gccgtgcccc
     1261 cccttccctt tgaaggtgac gacgaagcgg tcgaccgacg ccatcagcga gccgaatcgg
     1321 acgccgatct
//
LOCUS       DAOSWW010000865         1328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_92594_length_1328_cov_3.320503, whole genome shotgun sequence.
ACCESSION   DAOSWW010000865 DAOSWW010000000
VERSION     DAOSWW010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92594_length_1328_cov_3.320503"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..910
                     /locus_tag="PK442_13745"
     CDS             248..910
                     /locus_tag="PK442_13745"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO88762.1"
                     /translation="MKVPTMVEVIQAELKKRVLDGEFVPGDRLNVDELARVLNVSKTP
                     IREALGRLAREGIVQSKPRIGWRVSLLSMEEFFQYQETQHVIKMYLAEQIFPYLDKID
                     IPKLEKMNGNMHHFVETRQFERMLEENDRFHMEIFGVYPNKVLLEYLEEVNNTIRLQR
                     IHMMEKQLLSPESPLLQDAPKDHEEIIDAIKSGDRTRIIETSAKHQATILEALKRPGK
                     VS"
     gene            927..>1328
                     /locus_tag="PK442_13750"
     CDS             927..>1328
                     /locus_tag="PK442_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88763.1"
                     /translation="MTHPLWSPLRGKLRLAGLVSGSGNTLWKTLELQKQLEKSKEGSP
                     FEVVAVFADSGSASALEKAKSFGVPAVSLDIREFYARMNAPLKDREVRAAYDREILAM
                     LEPFSPDMLLLAGYVWATTDVITGSIPTVGVH"
BASE COUNT          304 a          384 c          378 g          262 t
ORIGIN      
        1 ttgccaatct tgatgacata cttctccgcc gccgccggga acgctaccga tagaagcacg
       61 ccgaccacgc ataccgcgca catcagaact cttttcatcg catccatctc ctttcatatc
      121 atggaacaca ccgtttggaa caccgtccgc cgcttctcct tcgttgacaa gaaatctgtt
      181 tcaagatact atcttgcata tcggatacta tatcagattt acccgtatcg ggaaaggggc
      241 gttttccatg aaggtcccaa ccatggtgga agtgattcag gcggagctga aaaagcgcgt
      301 cctcgacggc gagttcgtcc ccggggacag gctcaacgtg gacgagcttg ctcgcgttct
      361 caacgtgagc aagacgccga tccgcgaggc gctcggccgc ctggcccgtg aggggatagt
      421 gcagtcgaag ccccggatag ggtggcgggt ctcgctcctc tccatggagg agttctttca
      481 gtatcaggag acgcagcacg tgatcaagat gtaccttgcg gaacagatct tcccctacct
      541 cgacaagatc gacattccga agctggagaa gatgaacggc aacatgcatc actttgtcga
      601 gacgaggcag ttcgagcgga tgcttgagga gaacgaccgc ttccacatgg agatcttcgg
      661 cgtctacccc aacaaggtgc tcctggagta cctcgaagag gtcaacaaca cgatacgcct
      721 gcagcgcatc catatgatgg aaaagcagct cctctctccc gagagcccgc tgcttcagga
      781 cgcgccgaag gaccacgaag agatcatcga cgccataaag agcggcgaca ggacgcgtat
      841 tatagaaact tcggcaaaac atcaggccac catcctcgaa gcgctcaaac gcccggggaa
      901 agtgagctga acgggaggaa ttcgctgtga cacaccctct gtggagtccc ctgaggggga
      961 aactgcgtct ggccggactg gtctccggat cgggcaacac gttatggaaa acgttggagc
     1021 ttcagaaaca gcttgagaag agcaaagagg gctcgccgtt cgaggtggtc gccgtcttcg
     1081 ccgattccgg cagcgcatcc gcacttgaga aggcgaaatc gttcggggtt cctgccgtct
     1141 ctttggacat ccgggagttc tacgcccgga tgaatgcgcc gctgaaggac agggaggttc
     1201 gcgctgcgta cgaccgggag attctggcca tgctggagcc gttctccccc gatatgctgc
     1261 tgctcgcagg gtacgtctgg gcgacgacgg acgtcatcac cggctcgatt cccaccgtcg
     1321 gcgtccat
//
LOCUS       DAOSWW010000866         1327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_92709_length_1327_cov_3.121069, whole genome shotgun sequence.
ACCESSION   DAOSWW010000866 DAOSWW010000000
VERSION     DAOSWW010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92709_length_1327_cov_3.121069"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1327
                     /locus_tag="PK442_13755"
     CDS             23..>1327
                     /locus_tag="PK442_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88764.1"
                     /translation="MSLRQGVDHLASSLSKGADVFEKQCALVDTDSSQDRERKISKML
                     SLAKELSRAELAAQLAQCRAVADAPEGVVGFDDRQAMKELVARYLLALGEAEQALNAA
                     EDVRLSFGFSARSTGGMPNPEVVWRTLLVSAKASLALGAVPHAVRFLDQAIEVMESVS
                     PSDGTSSQATADKLDVYRTAVETAYSLYASSPSDEHAAMLWRYLEGMKSRQWREMLAT
                     TGAAFLDRLPESEEEHYQVLSMSAAQLLAKIAFLDFRGLAKEADEARAELQDVRGSLG
                     EITARYTVDSVRRVPTLEDASDALHPDWAAADYYLSRELSFAFVLEKGKRPRLVQLPL
                     DYDSFFAYTLWMRTLPDAVEFQSTRANEAVMAAGVSSPRLAKQLFEPVAQLIGEKKKL
                     LVIPHDLLYTFPFETMSVASGDTWRFLLDEGWTFAELPSAFLL"
BASE COUNT          256 a          364 c          421 g          286 t
ORIGIN      
        1 cgtcttggga gaggcaaaag cgatgtcgct gcgccagggg gtcgatcatc ttgcgagctc
       61 tctgtcgaag ggagccgacg tcttcgagaa gcagtgcgct cttgtggata ctgactcatc
      121 tcaagatcgg gaacgaaaaa tctcaaaaat gctctctctt gcaaaagaac tgtcgcgggc
      181 ggagctagcg gcgcagctcg cgcagtgcag ggcggtggcg gatgcgcccg aaggggttgt
      241 cgggttcgat gacaggcagg ccatgaaaga gcttgtcgcg cggtatcttc ttgcgctcgg
      301 agaggcggag caggcgctga acgccgcgga ggacgtgagg ctgagttttg gtttttcggc
      361 gcggagtact ggagggatgc cgaatcccga ggtcgtctgg agaacgctcc ttgtctccgc
      421 gaaggcctct cttgcattgg gcgctgtgcc gcacgcagtc cgttttctgg accaggcgat
      481 cgaggtcatg gaatccgtct ccccctccga cggcacgagt tcacaagcga ctgccgacaa
      541 gctggatgtg taccgtacgg ccgtagaaac ggcgtatagt ctctacgcct cgtccccttc
      601 cgatgaacac gcggcgatgc tgtggcgcta tctggaggga atgaagagca ggcagtggag
      661 agagatgctt gcgaccaccg gcgccgcgtt tcttgaccgt cttcctgaaa gcgaggaaga
      721 acactatcag gtactgagca tgtctgcggc gcagctgctg gcgaagatcg cctttcttga
      781 ttttcgaggg cttgcaaagg aggctgacga ggctcgcgcg gaacttcagg atgttcgggg
      841 atcgctcgga gagataacgg cccggtacac agtcgactcc gttcggcgag ttccgacgct
      901 tgaggatgct tccgacgcgc ttcatcccga ctgggcggcg gcagattact atctgtcacg
      961 cgagctctcc ttcgcatttg tgctggaaaa agggaagcgc cctcgccttg tgcagctccc
     1021 cctggactac gacagttttt ttgcgtacac gttgtggatg cgtacgcttc ccgacgcggt
     1081 agaattccag tcgacacggg ccaacgaggc cgtcatggct gcgggcgttt catccccaag
     1141 gctcgcgaag cagcttttcg agcccgtggc gcagctcatc ggcgagaaga aaaaactcct
     1201 cgtcatcccg catgatcttc tgtacacctt tccctttgaa accatgtcgg ttgcgagcgg
     1261 cgatacatgg aggttcctgc tggacgaagg gtggaccttt gcagaactcc cgagcgcgtt
     1321 tcttctt
//
LOCUS       DAOSWW010000867         1326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_92805_length_1326_cov_2.945712, whole genome shotgun sequence.
ACCESSION   DAOSWW010000867 DAOSWW010000000
VERSION     DAOSWW010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92805_length_1326_cov_2.945712"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PK442_13760"
     CDS             <1..365
                     /locus_tag="PK442_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOO88765.1"
                     /translation="ERIRAVVPCFSGTEEDARDFLEMGYFLSFGGPLTFPKNDALRAL
                     FRELPEEHILLETDSPWLTPHPFRGKRNEPMHVRLVYEKAAEIRGVPFASFAAAMESN
                     AARLFGWGTERTMGDRTS"
     gene            362..>1326
                     /gene="tgt"
                     /locus_tag="PK442_13765"
     CDS             362..>1326
                     /gene="tgt"
                     /locus_tag="PK442_13765"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOO88766.1"
                     /translation="MSAKAPLEFVVDAVCPVTGARAGRVVTQHGEIPTPVFMPVGTQA
                     TVKAMAPFELEEIGAKIILSNTYHLHLRPGGELVRAAGGLHNFMRWNHPILTDSGGFQ
                     VFSLASLGRISDEGVVCKSHLDGSTHVMSPEWSMETQLHLGSDIAMCFDQCAPYPCSR
                     EEAEEAVRRTTLWAGRSLEAHSRGIGEAPHQALFGIVQGSVHEDLRLRSAAELIPLDF
                     PGYAVGGLSVGEPAEEMYRILDVLNPVLPASKPRYLMGVGRPDNLVEGVARGIDMFDC
                     VLPTRNGRNGTLLTPEGAMNIKKKQYERDFSPVDPECDCYACRTFT"
BASE COUNT          232 a          438 c          420 g          236 t
ORIGIN      
        1 gggagcggat tcgcgccgtc gtcccctgct tctccgggac ggaggaagat gcgcgtgatt
       61 ttctggagat ggggtacttt ctctccttcg gcggcccgct cacatttcca aagaacgatg
      121 cgctgcgcgc gctcttccgt gaactgccgg aggagcatat cctgctggag accgactcac
      181 cctggctgac tccgcacccg ttcaggggca agcggaacga gccgatgcac gtcaggctgg
      241 tctatgagaa ggctgcggag atcaggggcg ttccctttgc ctctttcgcc gccgcaatgg
      301 agtcgaacgc cgcccgcctc ttcggatggg gtacggagcg gacaatgggg gacagaacgt
      361 catgagcgcg aaagctcccc tcgaattcgt cgtcgacgcg gtctgtccgg tcaccggcgc
      421 acgggccgga cgggtcgtca cgcagcacgg cgagattcca accccggtct tcatgccggt
      481 cggcacacag gcgaccgtca aggcgatggc gcccttcgaa cttgaggaga tcggcgcgaa
      541 gatcattctc tccaacacgt accaccttca cctgcgcccc ggcggcgaac tcgtccgagc
      601 ggccgggggg ctgcacaatt tcatgcggtg gaaccatccc atcctgacgg acagcggagg
      661 gtttcaagtc ttttccctcg cctcgctcgg ccggatctcc gacgagggcg tggtgtgcaa
      721 gtcgcacctc gacggttcga cgcacgtcat gtcgcccgag tggtctatgg aaacgcagct
      781 tcacctcgga agcgacatcg ccatgtgctt cgaccagtgc gccccgtacc cgtgctcgcg
      841 cgaggaggcg gaggaagctg tgcggcggac gaccctctgg gcaggccgct ctcttgaagc
      901 tcattcccga ggaatcggag aggccccgca ccaggccctc ttcggcatcg tgcaggggtc
      961 ggtccacgag gatctgcggc ttcgatccgc cgctgaactg atccccctcg attttccggg
     1021 gtacgctgtg ggggggcttt cggtcggcga acccgccgaa gagatgtacc ggatactcga
     1081 cgtgctcaat ccagtgctcc ccgcgtccaa accgcgctac ctgatgggcg tcggcagacc
     1141 ggacaacctg gtcgagggcg tcgcccgcgg catcgatatg ttcgactgcg tgctccccac
     1201 ccgcaacggc cgcaacggca cgctcctcac gccggaaggg gcgatgaaca tcaagaagaa
     1261 gcagtacgag agggacttct cgcccgtcga cccggagtgc gactgctacg cctgccgcac
     1321 cttcac
//
LOCUS       DAOSWW010000868         1323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_93173_length_1323_cov_3.279968, whole genome shotgun sequence.
ACCESSION   DAOSWW010000868 DAOSWW010000000
VERSION     DAOSWW010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93173_length_1323_cov_3.279968"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PK442_13770"
     CDS             complement(<1..1315)
                     /locus_tag="PK442_13770"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO88767.1"
                     /translation="MSYVAQRQSAAALLASAEDEGIALVRTLQEGFEQYLTARTAFME
                     AAAGHAEMKSGDWERQRAFLSSLDAKGMQIQAYFLIDLQGDGHYLDGKVNKLGDREYF
                     QRARDTRKTVVGQPVLSRATGETVVIVASPVFSEKGELLSVLCGRITANTLITLASTQ
                     KWGESGHALVTDANGLFIVHPDKEFIGAVNATQAGEKIPEALAAAAKRALGGEQGIAR
                     YVEGGAEQVAAYSRVPSTGWSVAMAASVDEFLAPVRAMRNVILTSLLVAALLVIAVSF
                     WIAASVSRPLARVVAKMDELATGDFSSSFEPRSSLVEMRHLSSSFNGMIASVADFAGG
                     VFGVSRKLLARAEDMSAASEQSSASVHEVIGLVGKVAQNSHDAASAIEEANAGVGEVA
                     HGAQAGAKAAVETGERAQEISTAAETGGSALEEMVHLIGEVSRSGA"
BASE COUNT          218 a          454 c          413 g          238 t
ORIGIN      
        1 tggctcccga acgggagacc tcccctataa ggtgcaccat ctcttccagt gcgctgccgc
       61 ctgtttccgc ggcagtggaa atttcctgcg cacgttctcc cgtctcgacg gcggccttgg
      121 cgcccgcctg cgctccgtgg gccacttcgc cgacgcccgc gttcgcttcc tcgattgcgc
      181 ttgccgcgtc atgcgagttc tgcgctacct tgccgacaag gccgataact tcgtgcaccg
      241 aggcgctgct ctgctccgag gccgcgctca tgtcctccgc acgcgcaaga agcttccgcg
      301 agacgccgaa gacgcctccc gcgaagtccg cgaccgaagc gatcatgccg ttgaacgagg
      361 aggaaaggtg tcgcatctcg acgagactgg aacggggctc gaaggaagag ctgaagtctc
      421 ccgtcgccag ttcgtccatc ttcgccacca cgcgcgcaag gggcctggag acgcttgcgg
      481 cgatccagaa cgagacggcg atgaccagca gcgccgccac gagcaacgac gtcagaatga
      541 cgttccgcat agccctgacg ggcgcgagga actcgtcgac ggatgcggcc atggccacgc
      601 tccatcctgt cgagggaacg cgcgaatagg ctgcaacctg ctccgctcct ccctcgacgt
      661 agcgcgcgat cccctgttct cctccgagag cgcgcttcgc ggcggcggcc aacgcctcgg
      721 gtatcttctc cccggcctgc gtcgcgttga cggcgccgat gaactccttg tcggggtgca
      781 caatgaagag tccgttcgcg tcggtgacga gggcatgccc gctctcgccc cacttctggg
      841 ttgaagcgag ggtgatcagc gtgttcgccg tgatccggcc gcagagcacg gagagcagct
      901 ctcccttctc gctgaagacg ggggaggcga cgatgaccac cgtttctccg gtcgcgcggg
      961 agaggacggg ctggccgacg acggtcttcc gggtatcgcg ggcgcgctgg aagtactcgc
     1021 ggtcgcccag cttgttcact ttgccgtcga gatagtgtcc gtctccctga agatcgatga
     1081 ggaaatacgc ctggatctgc attcctttcg catccagaga ggagaggaag gcccgctgac
     1141 gctcccagtc gccgcttttc atctcggcgt ggccggccgc ggcctccatg aacgcggtcc
     1201 gcgcggtgag gtactgctcg aacccctcct gcagagtacg cacgagggcg attccctcat
     1261 cctccgcgga tgcaagaagc gccgccgccg actgacgttg cgcgacgtag ctcactccgc
     1321 cca
//
LOCUS       DAOSWW010000869         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_93321_length_1322_cov_2.720600, whole genome shotgun sequence.
ACCESSION   DAOSWW010000869 DAOSWW010000000
VERSION     DAOSWW010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93321_length_1322_cov_2.720600"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK442_13775"
     CDS             <1..454
                     /locus_tag="PK442_13775"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88768.1"
                     /translation="PSFAEAHLLVGKAYFLLGDNHKATPPLDKALELDPRLVEAIFLK
                     GSAFKNRKRVEEAADYFLEAIQIDKTYIPAYLALGQLLLENGAADEASGILLSGLQQQ
                     PNNGNLRFLLGAAQIMRGNLQGAKEQLSALKTIDAALAKKLEDLLPKQ"
     gene            694..1233
                     /locus_tag="PK442_13780"
     CDS             694..1233
                     /locus_tag="PK442_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88769.1"
                     /translation="MIRLATTVSGEGGCIASYVGGASLMRNGEYEQGRDLALPVLDRC
                     YGASVQFWKDSERLRTLVAGEVSLHAGGQGDYGTIITMEDKLGKDILTDRLLVRDAKG
                     ILEGRTRLGDVLRFHKARAYTTAQMPNEAKQTLKELEFASGKVFVDGEVTGLKDAIAK
                     LKIQVEGMARLILSFFRAA"
BASE COUNT          304 a          360 c          412 g          246 t
ORIGIN      
        1 accgtcgttc gcggaggcac acctgctcgt cggcaaggcg tactttctcc tcggggacaa
       61 ccataaggcc acgccgccgc tggacaaggc gctcgaactc gatccccgcc tcgttgaggc
      121 gatctttctg aaggggtcgg cattcaagaa cagaaagagg gtcgaggaag cagcggatta
      181 cttcctggag gccattcaga tcgacaagac ctatattccg gcctatctgg ccctcgggca
      241 gcttctgctt gagaacggcg cggcggacga agcctccgga attttgcttt cggggctcca
      301 gcagcagccg aacaacggca atctccgctt ccttctgggc gctgcccaga tcatgcgggg
      361 aaaccttcag ggagcgaaag aacagctttc cgccctgaag acaatagatg cggcgcttgc
      421 gaagaaactg gaggatttgc ttcccaaaca gtgatcgaga acagaaagga tgtggcggta
      481 atgagaaaga aatttgtact tcttctgagc gtttttgtac ttggcggcgc gctgcttctg
      541 gcgcccgtcg cggggttcgg ctgaggaggc ggcggcaccg gaggttccgg tggcagcgac
      601 agcactccct cggcggccga tcagaaggaa atcgacaaac tggtacagat ttttttgaac
      661 gagggaacgc tcagcggacg caatcttgac cggatgattc gacttgcaac aaccgtaagc
      721 ggcgaaggag ggtgtatcgc atcctatgtc ggcggagcga gcctgatgcg gaacggcgag
      781 tacgaacagg gacgcgatct cgcgctcccc gttttggaca gatgctacgg cgcgtcggtc
      841 caattctgga aagacagcga acgcctgaga acactggtcg ccggggaggt ttcccttcac
      901 gccggaggac agggcgacta cggaacaatt atcacgatgg aggacaaact cggaaaggac
      961 atactgaccg acaggctgct ggtccgcgac gcgaaaggca tcctcgaggg gcggacccgc
     1021 ctcggcgacg ttctccgctt ccacaaggcg cgcgcgtaca ccacggcaca gatgccgaac
     1081 gaagcgaagc agacgctcaa agagcttgag ttcgcctccg ggaaggtctt cgtggacggc
     1141 gaggtcaccg gactgaaaga cgccatcgcc aaattgaaga tacaggttga aggcatggcg
     1201 cgcctgattc tctcgttctt cagggctgca tgatctgcgg agcggaaacg accctatcac
     1261 cttgctacaa aggggggatc gaggatgtcc tgtaaaaaag cgtgtcggct aattctgctg
     1321 tt
//
LOCUS       DAOSWW010000870         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_93338_length_1322_cov_2.449092, whole genome shotgun sequence.
ACCESSION   DAOSWW010000870 DAOSWW010000000
VERSION     DAOSWW010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93338_length_1322_cov_2.449092"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..817)
                     /locus_tag="PK442_13785"
     CDS             complement(143..817)
                     /locus_tag="PK442_13785"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO88770.1"
                     /translation="MENVTFERDTLSERVADFIRRQILYTDVFKDGDHILETDIADHL
                     QVSRATVREALKDLETQGIVEIIPRKGTYVAAFDHKDMIEILELRCMCEAYIFETVVN
                     GDMLDARDFAVLESIIDEMAQVSRSAEESELAKSIAFTTKDLEFHGYIWKKSGRRWFC
                     KVLSDNYYRLRLAMMQDMILERDMEKSATMHYDILNALREKDLDAARNYLRRHILSLH
                     GAETVA"
     gene            complement(914..>1322)
                     /locus_tag="PK442_13790"
     CDS             complement(914..>1322)
                     /locus_tag="PK442_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOO88771.1"
                     /translation="HCPISRTLFLGEPPAELRKIADAVVKGLNETVAFIRPGVTAEAV
                     EEKWRESIAGTGVHKPSRVGYSFGLNYPPDWGEQTLSLRPADTTILQPGMCIHFMPGI
                     WLKDFGFECSEPLVVTGSGCELFLNFERKLFVK"
BASE COUNT          220 a          415 c          391 g          296 t
ORIGIN      
        1 tccccgtctt ctccatgaac gcctgaatct ccctgaggtg tttcgccttg aacatccctg
       61 aagcgcctcc ttttaatgtc gtcatttttt tgtcgacacg ctctccgccg gtaaaaaaag
      121 cccgcacggg gcaggctcgt cgtcaggcga ccgtttcggc gccgtggagc gagagaatgt
      181 gtctgcgaag gtagtttctg gccgcgtcca ggtccttctc ccggagcgcg ttcagaatat
      241 cgtagtgcat cgtggccgac ttctccatgt cccgttcgag gatcatgtcc tgcatcatgg
      301 ccaaccgcag cctgtagtag ttgtccgaga gcaccttgca gaaccagcgc cggccggact
      361 tcttccagat gtagccgtgg aactcaaggt ccttcgtggt gaaggcgatc gacttggcga
      421 gctcgctctc ctccgccgag cgggagacct gcgccatctc gtcgatgatc gattcgagga
      481 cggcgaagtc gcgcgcgtcg agcatgtccc cgttcacgac cgtctcgaag atgtacgcct
      541 cgcacatgca gcggagttcg aggatctcga tcatgtcctt gtggtcgaag gccgcgacgt
      601 acgtcccctt gcgggggatg atctcgacga tcccctgcgt ctccaggtcc ttgagggctt
      661 cgcggaccgt ggccctgctc acctgcaggt gatcggcgat atcggtctca aggatgtggt
      721 cgccgtcctt gaagacgtcc gtgtacagta tctgccgccg gatgaagtcg gcgacccgct
      781 cgctcaaagt atccctttcg aaggtgacgt tttccatgac gcgctctccg tttcccgggg
      841 agaagatcac cgctccccgt tcgttctctc tggtctattg tatgtggtcc gctctcttat
      901 tctatcattt cggtcatttg acaaacagtt ttcgctcgaa gttcaggaag agctcgcacc
      961 cgctcccggt gacgacgagc ggctcgctgc actcgaagcc gaaatccttc agccagatgc
     1021 ccggcatgaa gtggatgcac atccccggct ggaggatggt cgtatccgcc gggcggaggc
     1081 tgagcgtctg ctcgccccag tcgggcgggt agttcaggcc gaaggagtac ccgacgcgcg
     1141 acggtttgtg cacgcccgtt ccggcgatgg actccctcca cttctcctcc accgcctcgg
     1201 ccgtgacgcc cggacggatg aaggcgaccg tctcgttcag gcccttgacg acggcgtccg
     1261 cgatctttcg aagctccgcg ggcggctccc cgaggaagag cgtgcgcgat atggggcagt
     1321 ga
//
LOCUS       DAOSWW010000871         1320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_93533_length_1320_cov_5.049802, whole genome shotgun sequence.
ACCESSION   DAOSWW010000871 DAOSWW010000000
VERSION     DAOSWW010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93533_length_1320_cov_5.049802"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /gene="nrdD"
                     /locus_tag="PK442_13795"
     CDS             <1..431
                     /gene="nrdD"
                     /locus_tag="PK442_13795"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOO88772.1"
                     /translation="HFPYNILMDPVRKIVEEGRFHPLISAGAITHIWMGEHKPDARAL
                     SSFIRKVFRHSENAQVTFSPEFTVCNGCGVTSRGLRESCPSCGSMDVDGITRITGYFT
                     RTSSWNDGKRAELQDRVRTSIPDVPSEVGLSRQPSLALEK"
     gene            522..>1320
                     /locus_tag="PK442_13800"
     CDS             522..>1320
                     /locus_tag="PK442_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88773.1"
                     /translation="MKLRNVLLAALLVFVVAGTAMAADTIKIGVYLPLTGRTAFGGQL
                     ELEGIQMAHKEMSEVLGKKVELVVVDNKTDKVEAANAVKRLIEYDKVVAVIGTYGSSL
                     AMAGGEVAEAAKVPMMGTSCTNPLVTQGKMYVFRACFIDPFQGAGAATYAINSLKMNK
                     AALLIDVANDYCVGLANFFKQSYKKLGGEIVSELKYNSGDQDFTAQLTEIISQNPDLL
                     FIPADFAEGAIIMKQARELGAEFQIMGGDAMDNPEIVNIGGEAVEGFI"
BASE COUNT          312 a          338 c          367 g          303 t
ORIGIN      
        1 cgcacttccc gtacaatatc cttatggatc ctgtgcggaa gattgtagag gagggacgtt
       61 ttcatccgct tatctccgcc ggggccatta cgcacatttg gatgggcgag cataaaccgg
      121 atgcccgcgc tctttcttcg tttatacgga aagtttttcg tcactccgaa aacgcgcaag
      181 ttactttcag cccggaattc actgtttgca acggctgcgg cgtgacgtcg cgcggtcttc
      241 gagagagttg tccttcctgc ggaagtatgg acgtggacgg tattacgcga attaccgggt
      301 attttaccag gacttcgagc tggaacgacg gaaagagggc tgagcttcag gacagggttc
      361 gtacctcgat tcccgatgta cccagtgaag tcggtctttc gcgacaacct tccctcgccc
      421 ttgaaaagta aaaataaaga aaagaaaggg cttccggaag tcccagtttt tgtgtataat
      481 ttatcgtgaa gtcgaatatc aatatctgag gagggaaccg aatgaagctt cgaaatgtac
      541 tgcttgctgc actgttggtt ttcgttgttg cgggaacggc tatggccgcc gatacgatca
      601 agatcggcgt atatcttcct ttgaccggaa gaaccgcttt cggcggacag ttggaacttg
      661 aaggcattca gatggcgcac aaggaaatgt cggaggtgct tggaaagaaa gtcgaacttg
      721 tcgtcgtcga caacaagacg gataaggtcg aggccgccaa cgccgtgaag cgccttatcg
      781 aatatgataa ggtcgttgcc gtcatcggaa cgtacggatc ctctctcgcc atggcgggcg
      841 gcgaggtcgc cgaagccgcg aaggtcccca tgatggggac ctcctgcacc aacccgctgg
      901 ttacccaggg caagatgtat gtcttcagag catgcttcat cgatcccttc cagggagcgg
      961 gcgctgcgac gtatgcaatc aactctctca aaatgaacaa ggccgcgctt ctcatcgacg
     1021 tggcgaacga ctactgcgtg ggacttgcca atttcttcaa gcagtcctac aagaagcttg
     1081 gcggcgagat cgtatcggag ctgaagtaca actccggcga tcaggacttt actgcgcagc
     1141 ttaccgagat catcagccag aatcctgacc ttctcttcat cccggccgac tttgccgagg
     1201 gcgccattat tatgaagcag gccagagagc tgggcgccga gttccagatc atgggcggcg
     1261 acgccatgga caaccccgaa atagtcaata tcggcggaga agctgttgaa ggcttcatcc
//
LOCUS       DAOSWW010000872         1320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_93577_length_1320_cov_2.702767, whole genome shotgun sequence.
ACCESSION   DAOSWW010000872 DAOSWW010000000
VERSION     DAOSWW010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93577_length_1320_cov_2.702767"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PK442_13805"
     CDS             complement(<1..1041)
                     /locus_tag="PK442_13805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO88774.1"
                     /translation="MSTERASYGKSFWISMAVIFLTQAFLAVFMLYPSVLRDQGFSLG
                     LSGWLMSVFNLCSTLGRPLGARATERLGIRTTLLWTSGLVAVFTMPLAFTEHPLLLLF
                     LRGTGGVFWGISMVAISSYQGLSIPAARRGSAYAWIAVAYVLPQVTLFPLADWFASSR
                     MLTAYFLLAAAVEAAIVLVAAPLPPLRRAESSQEEPKEWGRWSELPSLPGFRAVLATM
                     FLFAFVNASTLQYLPAFLSMRGYSPSVFIVPNAMTAIALRLVAFRIMDRIDRRRVIGS
                     IIAAMGFILAFLPFAPGRGWYVAGGLFYGVVMGMCFPIVLALMPDLFPERLRPKGISL
                     CLFAIDLGFILSP"
     gene            complement(1135..>1320)
                     /locus_tag="PK442_13810"
     CDS             complement(1135..>1320)
                     /locus_tag="PK442_13810"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88775.1"
                     /translation="ICLGALTPPVGTCLFVASSVGGVNLTKTSRAILPIVLLAVFCLA
                     LAIFFPAVILFLPSLLR"
BASE COUNT          318 a          358 c          447 g          197 t
ORIGIN      
        1 gggggagaga ataaacccga ggtcgatagc gaaaaggcag agagatatcc ctttcggacg
       61 cagacgctcg ggaaagaggt ccggcatcaa cgcgagcact atggggaagc acatccccat
      121 gacgacgccg tagaacaacc ccccggcgac gtaccagccg cgtcccggtg cgaaggggag
      181 gaaggccaat atgaatccca ttgcggcgat gatgcttccg atcacccgtc tgcgatcaat
      241 ccggtccata attcggaagg ccacgaggcg cagggcgatc gcggtcatcg cgttaggaac
      301 gatgaagacg ctcggcgagt accctctcat ggagaggaag gccggaaggt actggagtgt
      361 ggaggcgttg acgaaggcaa agaggaacat ggtggcgagc accgcccgga atccggggag
      421 cgaggggagt tcgctccatc tcccccactc tttcggttcc tcttgcgaac tctccgctcg
      481 tcgaagcggg ggaagcggcg cggccaccag gacgatggcg gcttcgaccg ctgcggcgag
      541 caggaagtag gccgtgagca tgcgggagga agcgaaccag tccgccagag ggaaaagagt
      601 gacctgagga aggacatacg ccacggcgat ccatgcgtat gcgctccctc ttcgcgctgc
      661 ggggatactg agcccttggt acgaggagat ggcaaccatg gagatccccc agaagacgcc
      721 gcctgttccc cggaggaaga gaagcagcag cgggtgttcc gtgaaggcga gcggcattgt
      781 gaagacggcg acgagcccgg aggtccagag cagcgtggtg cggatcccga gccgttccgt
      841 cgctcgcgct cccagaggcc tgcccagcgt ggagcagagg ttgaacacgc tcatcagcca
      901 tcccgagagg ccgagagaga acccctggtc gcggagcacg gaggggtaaa gcatgaagac
      961 ggcgaggaac gcctgcgtaa gaaagatcac cgccatcgag atccagaaag attttccgta
     1021 agaagcgcgt tcagtggaca tagtctctcc tttgctctgc ttcgaaatac gtagcagtgc
     1081 aggcggtccg cagatttcgg gacatgggtg cgaaccgccg tgtttttagg gatatcaccg
     1141 caacagcgag ggcaagaaga gtataacagc aggaaagaaa atggccagcg ccaggcagaa
     1201 taccgcgagc aggacgatag gcagtatggc tctgctcgtc tttgtgaggt tgacgccgcc
     1261 gacgctcgag gcgacgaaaa gacatgttcc caccgggggc gtgagagcgc cgagacagat
//
LOCUS       DAOSWW010000873         1319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_93740_length_1319_cov_2.341772, whole genome shotgun sequence.
ACCESSION   DAOSWW010000873 DAOSWW010000000
VERSION     DAOSWW010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93740_length_1319_cov_2.341772"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PK442_13815"
     CDS             complement(<1..676)
                     /locus_tag="PK442_13815"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOO88776.1"
                     /translation="MSIPSPYNFVPLSDKVYFPDWADRVSMDVPFSDGISGTLEIKVT
                     AKTPIYIRNGGAHPEDSAGKRNSAQYKDFFRASPDGPYAIHGSSLKGMLRNVVEIITY
                     SKIAGTGKDTARVSEHRYAIRDLYNKDYTTEITEQTPQGYKPKVRAGWLSGKDGEWKV
                     SLCDFARVEQESLETYFGQKEKRAISLGDRGPAEGKYRQIPEYTTIKFTCGRETGHLH
                     SGGNKLV"
     gene            complement(673..1164)
                     /locus_tag="PK442_13820"
     CDS             complement(673..1164)
                     /locus_tag="PK442_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88777.1"
                     /translation="MNTNTTQKTLADCLAEPEAFVPGRLETTSEDSFDAAHVLEKFLS
                     FAGEGWLQIAEEEEMRVFSPDSPLKEPKGWPVAGEAASSDKSISLVREGTGWKLITTE
                     KLPAETTDDLISTTSFRRRDAEGSLRYETCWKAIDVFGQTEIRPVAYRFAGFSSGIKE
                     DRA"
BASE COUNT          210 a          376 c          389 g          344 t
ORIGIN      
        1 aaaccagttt gttccctccg ctgtgaagat gtcccgtttc ccgtccgcat gtgaatttga
       61 ttgttgtata ctccgggatc tgtcggtatt tcccttccgc agggcctcga tcgcccagcg
      121 atattgctct cttttctttt tggccgaagt aggtttcaag agattcctgc tctacgcgcg
      181 caaagtcgca gaggctcacc ttccattctc catctttgcc ggagagccat cctgcgcgga
      241 ctttgggctt gtacccctgc ggtgtttgtt cggtgatctc ggttgtgtag tccttgttgt
      301 acaggtcgcg tatcgcgtat cgatgttcgc tgactctggc ggtgtctttg ccggtacccg
      361 cgatcttcga gtaggtgatg atttcgacga cgttgcgcag catccctttc agcgacgagc
      421 cgtggatggc gtaggggccg tcgggggagg cgcggaagaa gtccttgtac tgcgcgctgt
      481 ttctcttccc tgcgctgtct tccggatggg ctcctccgtt ccggatgtag atcggcgttt
      541 tggccgtgac ctttatttca agcgtgccgc tgatgccgtc cgagaagggg acgtccatgg
      601 aaacgcggtc ggcccagtcg gggaagtaga ctttatcgga gagcggaacg aagttgtacg
      661 gagacggtat gctcatgctc tgtcctcctt tatgccggat gagaagcccg cgaagcgata
      721 cgcgacgggg cgtatctcgg tctgtccaaa gacgtcgatg gctttccagc aggtttcgta
      781 ccggagactg ccttccgcgt cccgtctccg gaaactcgtg gtcgagatca gatcgtcggt
      841 ggtctctgcc ggaagctttt ccgtcgtgat gagtttccaa ccggttcctt cgcggacaag
      901 cgagatgctt ttgtcggatg atgcggcttc tccggcgacg ggccaccctt tcggttcttt
      961 gagcggagaa tccggggaaa agacgcgcat ctcctcctcc tcggcgatct ggagccatcc
     1021 ttcgccggcg aaactgagaa atttttccag aacgtgtgcg gcgtcgaaag agtcttccga
     1081 cgttgtttcc aaccgtcccg gaacaaatgc ttccggttcg gccagacagt ccgcgagcgt
     1141 tttttgcgtc gtgttcgtgt tcatcctctt cacccctcga tggctgaacg tatgtccgca
     1201 ggccagcgga cgggagcttc cgcgtcgaag tagcccagtc cgcgcccgtt ccccgcgcct
     1261 acctgaaggc gtccttcggc gagatcttcg acggccgcgc ggaatgcttt taggacgtc
//
LOCUS       DAOSWW010000874         1318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_93855_length_1318_cov_2.329375, whole genome shotgun sequence.
ACCESSION   DAOSWW010000874 DAOSWW010000000
VERSION     DAOSWW010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93855_length_1318_cov_2.329375"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK442_13825"
     CDS             <1..308
                     /locus_tag="PK442_13825"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOO88778.1"
                     /translation="AASMARCLPRCAAFVQCAPPLKARFLRARWGSWSSARGVVLNTH
                     LLKAPPECIDYVLLHELCHVRHPNHGKGFYELLESVSPEWKELKKKLEETVHLRLFA"
     gene            complement(518..>1318)
                     /locus_tag="PK442_13830"
     CDS             complement(518..>1318)
                     /locus_tag="PK442_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO88779.1"
                     /translation="PCFSPYFEQIYQHRGVAVHLPTTEVQGFVPSEDDIERLVTPRSR
                     VLMICSPSNPTGRVMKREQMESIARAAEKHDLVVLSDEIYDSMVYTGKHVPFATVPGM
                     KERTLTMGGLSKSHCMTGWRIGYAIGPAPLIKVMSLIGANQTYGVNVPTQRAALYALE
                     HHDAKMEERARVFRDRLAYVAKRLNAMKGVRCAEPEGAFYLFPDIRGTGVDSEQFAWG
                     LLEKGHVATLPGSAFGACGEGYVRIACTRSMEMLEKGMDRMEQFVKSL"
BASE COUNT          234 a          409 c          438 g          237 t
ORIGIN      
        1 tcgcggcaag catggcgcgc tgcctcccgc gttgcgcggc gtttgtccag tgcgcgcccc
       61 cgttgaaggc gaggtttctt cgggcgcgct ggggaagctg gtcgagcgcg cgcggcgtcg
      121 tgctgaacac gcacctgctg aaggccccgc ccgagtgcat cgactacgtg ctgctccacg
      181 aactctgcca cgtccgccac ccgaaccacg gcaaaggctt ctacgagctg ctggaaagcg
      241 tgtcccccga gtggaaggaa ctgaagaaga agctggagga gactgtccac ctgcgcctct
      301 ttgcgtgaag gggacactta caacaggtca aggcgccctt ctttccatat tcaacaagaa
      361 tcgtgattcg aagtgaatcg aatcggaaag aacggcgagt ggaaaacgaa cgccggaggc
      421 actgtgattc ccggggcgaa cgatgttttg gcaaacaaca caaggggaag gagcgcgccc
      481 cttccccttg tcgctgttct tcctgagaat ccttccgtta gagcgacttc acgaactgct
      541 ccatccggtc cattcccttt tctagcatct ccatcgagcg ggtgcaggcg atgcggacgt
      601 acccttcgcc gcaggcgccg aaggccgatc cggggagggt ggcgacgtgc cccttctcca
      661 gcagccccca cgcgaactgt tcgctgtcga cgcccgtgcc gcggatgtcg gggaagaggt
      721 agaaggcccc ctccggctcc gcgcagcgga cgcccttcat cgcgttcagc cgcttggcga
      781 cataggcgag gcggtcgcgg aagacgcggg cgcgctcctc catcttcgcg tcgtgatgtt
      841 cgagcgcgta gagggcggcg cgctgggtgg gcacgttgac gccataagtc tggttcgccc
      901 cgatgagcga catgaccttg atcagcggcg cggggccgat ggcgtacccg atgcgccacc
      961 ccgtcataca gtggctcttc gagaggccgc ccatggtgag ggtgcgctcc ttcatgccgg
     1021 ggacggtggc gaaggggacg tgcttccccg tgtagaccat cgagtcgtag atttcgtcgg
     1081 agaggacgac gaggtcgtgc ttctccgcgg cgcgggcgat ggactccatc tgctcgcgct
     1141 tcatcacgcg cccggtgggg ttgctcggcg agcagatcat cagcacgcgc gagcgcggcg
     1201 tgaccagccg ttcgatatca tcctccgacg ggacgaaccc ttgcacctcg gtggtgggca
     1261 gatggaccgc gacgccccgg tgctggtaaa tctgctcgaa gtagggggag aagcaggg
//
LOCUS       DAOSWW010000875         1311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_94778_length_1311_cov_2.225318, whole genome shotgun sequence.
ACCESSION   DAOSWW010000875 DAOSWW010000000
VERSION     DAOSWW010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94778_length_1311_cov_2.225318"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1311)
                     /locus_tag="PK442_13835"
     CDS             complement(<1..>1311)
                     /locus_tag="PK442_13835"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOO88780.1"
                     /translation="SRVRREYVISPGRIEGRGRRGKSLTADYVLEFRNAKLAVVEAKS
                     RDEPLTAGAAQAKNYAEKLQVRFAYATNGQGIYEIDMMTGREREIPFYPSPEDLWRRI
                     HAVQNEWRDRFFEVPFEDKSGSHPGRYYQDIAVERVLHSVAEGKKRILLTLATGTGKT
                     FIAFQVAWKLFHSRWNLSREPARRPRILFLADRNILADQAYNAFSSFPEDAMVRISPA
                     DISKKGRVPKNGSLFFTIFQTFMTSPRRDEESSFCFGEYAPDFFDFIIVDECHRGGAN
                     DESAWRGILEYFAPAVQLGLTATPKRAENIDTYAYFGEPVFVYSLKEGINDGFLTPFR
                     VKQIATTLDEYFYTPDDTLVEGEVEQGKIYTESDFNRIIEIKERERKRVRIFMEQIDQ
                     REKTLVFCANQEHALFVRDLVNQTKTSADPNYCHRVTADDGELGE"
BASE COUNT          246 a          391 c          375 g          299 t
ORIGIN      
        1 ttcgccgagc tcgccgtcgt cagcagtcac tcgatggcag tagttcgggt ccgcgctcgt
       61 ttttgtctgg ttgacgagat cgcggacaaa gagcgcgtgt tcctgattcg cgcagaaaac
      121 aagcgtcttc tctctctggt cgatctgctc catgaaaatg cgcacgcgct ttctttcgcg
      181 ctccttaatc tcgataatcc tgttgaagtc gctctcggtg tagattttcc cctgctccac
      241 ctccccttcg acgagcgtat cgtcgggggt gtagaagtac tcgtcgagcg tggtcgcaat
      301 ctgcttcact ctgaaaggcg tcaaaaaacc gtcgttgatt ccctccttca gagagtacac
      361 aaagacgggc tctccgaagt aggcataggt gtcaatgttc tcggcccgct tcggcgtcgc
      421 ggtgagtccg agctgcaccg ccggtgcaaa gtattccaga atgccgcgcc acgcgctctc
      481 gtcgttggcc ccgcctctgt ggcactcgtc gacgatgatg aaatcgaaga aatcgggagc
      541 gtactcgccg aaacagaaag aggactcttc atcccttcga gggcttgtca tgaaagtctg
      601 gaagatggtg aagaaaagac tgccgttctt cggcacgcgt cctttttttg aaatgtccgc
      661 aggggagatc cgaaccatcg catcctccgg gaacgaggag aatgcgttgt acgcctggtc
      721 ggcgaggatg tttctgtccg cgaggaagag gatgcggggg cgtctcgcag gctcgcggga
      781 aagattccat cgactgtgaa agagcttcca tgcaacctga aaggcgatga aggtctttcc
      841 cgtaccggtg gcgagcgtca gcaaaatgcg ctttttcccc tccgctaccg agtgcagcac
      901 ccgctcgacc gcgatgtcct ggtagtaccg tccggggtgg gaaccgctct tgtcctcgaa
      961 agggacctcg aaaaaacggt cgcgccactc gttctgcacg gcgtggattc ggcgccatag
     1021 atcttccggg gaggggtaga acggaatctc ccgttccctg cccgtcatca tgtcgatctc
     1081 gtaaatcccc tgcccgttcg tagcataggc aaaacgcact tgcagttttt cggcgtagtt
     1141 cttcgcttgg gctgcgcccg cggtgagggg ttcgtcccgc gacttcgcct cgaccacggc
     1201 cagcttcgcg ttccggaact ccagcacgta gtcggcggtc agcgacttgc cccgccttcc
     1261 gcgcccttcg attcgccccg gcgagatgac atactcccgt ctgacgcggc t
//
LOCUS       DAOSWW010000876         1311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_94785_length_1311_cov_2.153662, whole genome shotgun sequence.
ACCESSION   DAOSWW010000876 DAOSWW010000000
VERSION     DAOSWW010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94785_length_1311_cov_2.153662"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PK442_13840"
     CDS             complement(<1..252)
                     /locus_tag="PK442_13840"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOO88781.1"
                     /translation="MIKLFTIGCSGRSAQDFFGALGKAQIQTVIDIRLRNTSHLAGFS
                     KMQDLPFFLESICGAAYIHIPQLAPTERILDDFKKNKGSW"
     gene            complement(249..842)
                     /locus_tag="PK442_13845"
     CDS             complement(249..842)
                     /locus_tag="PK442_13845"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOO88782.1"
                     /translation="MTIFTIGHSDHSPDVFVELLLRHGIEAVADIRSAPFSRRFPAFN
                     KESLRSILAKRGIAYVFLGKELGARPAAPSCYRDGRAEFDLIAGSEAFRNGIARLLKG
                     ASRMRIVLLCAEKEPLHCHRCILAGRVLAEQGEDVVHILADGSAERHEETMQRLLVLY
                     GKTEDDLFLSPQERVRLVCEMQEKKMAHMLKNGDEDE"
     gene            complement(851..>1311)
                     /locus_tag="PK442_13850"
     CDS             complement(851..>1311)
                     /locus_tag="PK442_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88783.1"
                     /translation="SKRWGKWEFVPFGLDSLAVLEDSPESLWVDGYHSGKGLNDKVPE
                     SVTSGLTSSLYLIRPSNLRLHITEEEKKTSHRASFQYHGKGYNLKITDLRFDNMLPSN
                     VRSLNTEDTWLTISLGLPFETRSASAYSDACRASYKLVAAVFLREFFEKR"
BASE COUNT          295 a          399 c          323 g          294 t
ORIGIN      
        1 ccagcttccc ttgttttttt tgaaatcatc caggatcctc tccgtagggg ccagctgggg
       61 aatatgaata tacgcggccc cgcagatgct ctcaaggaag aacggcagat cctgcatctt
      121 tgaaaaaccg gcgagatgcg atgtgttgcg aaggcggata tcgatcacgg tttgtatttg
      181 cgccttcccg agcgcgccga aaaaatcctg ggcgcttctg cccgaacatc ctatcgtaaa
      241 cagcttgatc attcgtcttc atccccattc ttgagcatgt gcgccatctt cttctcctgc
      301 atctcgcaca caagccgtac acgctcctgc ggagagagaa acagatcgtc ttctgttttg
      361 ccataaagca ccagcaaccg ctgcatggtc tcttcatggc gctccgccga accatccgcc
      421 agaatatgaa caacatcctc cccctgttcg gcaagcactc gccccgcgag aatgcaccgg
      481 tggcagtgca agggctcctt ctccgcgcac agaagaacta tacgcatgcg ggaagctcct
      541 ttcagaagcc gtgctatccc gttccggaac gcttcgcttc cagcgatcag gtcgaactcg
      601 gcgcggccgt ctctgtagca ggacggggcg gcgggacgcg ctcccagctc ctttccaaga
      661 aaaacgtagg cgatacctct cttcgccaga atactgcgca gagattcttt gttgaaggcc
      721 gggaaacggc ggctgaaagg agccgagcga atgtccgcga ccgcctcgat gccgtgtcgc
      781 agcaaaagct ccacaaaaac atcgggcgag tgatccgaat gtcctatggt gaatatcgtc
      841 acgccttgct tcacctcttc tcgaaaaact cccgcagaaa aaccgccgca acaagcttat
      901 aacttgcgcg gcaggcgtcc gaataagcac tggcgcttcg tgtctcgaac ggaagcccca
      961 ggctgatcgt tagccatgtg tcctcggtat tcagcgatcg tacgttggat gggagcatgt
     1021 tgtcgaagcg caagtcggtt atcttcagat tgtacccctt cccatgatac tgaaacgacg
     1081 cccggtgtga cgttttcttt tcttcctccg tgatatgcag gcgaagattc gacgggcgta
     1141 tcagatacag cgacgatgtc aatccgctcg tcacgctctc cggaaccttg tcgttcagtc
     1201 cctttccact gtgatacccg tcgacccaca atgactcggg cgagtcctca agaaccgcca
     1261 gcgaatctaa accgaacgga acaaactccc actttcccca tcttttcgat g
//
LOCUS       DAOSWW010000877         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_94887_length_1310_cov_2.450996, whole genome shotgun sequence.
ACCESSION   DAOSWW010000877 DAOSWW010000000
VERSION     DAOSWW010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94887_length_1310_cov_2.450996"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK442_13855"
     CDS             <1..202
                     /locus_tag="PK442_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88784.1"
                     /translation="RAPLSHIPLLATGGVNEETLCGFLDAGCAGAGVGGRLADKKLLA
                     EKNYGEIRKRAEAFVSLLQGRA"
     gene            216..1238
                     /locus_tag="PK442_13860"
     CDS             216..1238
                     /locus_tag="PK442_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOO88785.1"
                     /translation="MGRIVCFGEVMLRLGPEGYYRFHQADRFVLNYTGAEANAAVALS
                     QFGMETEIVTKLPDHAIAKAAVAELKKFGAGTRFIQFGGERMGVYFIEKGASQRPSKV
                     IYDRKNTSISAVRPGEFDWETIFEGASAFLFSGITAALSDTMPDVLAEACEKAKEKGV
                     KIFCDLNYRKNLWTPEKAQITMKRLVQYVDVLVGNEEDTEKMLGIKAGESDVHKGELD
                     TASYVDVAKQLQREYGFEAVGTTLRKSISASDNKWSGMLYTNGTAYLSREYDIRIVDR
                     VGGGDSFTSGLMYAMLNGYDSRKALEFAVAASCLKHSIELDFNLVSVEEIVALMEGDG
                     SGRVQR"
BASE COUNT          289 a          360 c          449 g          212 t
ORIGIN      
        1 gcgggcgccg ctcagccaca ttccgctgct ggccaccgga ggagtgaacg aggagacgct
       61 gtgcggattc ctggacgccg ggtgcgccgg agcgggcgtg ggcgggcgtt tggccgacaa
      121 gaagctcctc gcggagaaga actacggcga aatccggaag agagccgagg cgttcgtctc
      181 gctgcttcag ggaagggcgt aaggaggggc gcggcgtggg aaggatcgtc tgtttcggcg
      241 aggtgatgct ccgtctgggc cccgaagggt actaccgctt ccatcaggcg gacaggttcg
      301 tcctcaacta cacgggggcg gaggcgaacg ccgccgtggc gctgtcgcag ttcggcatgg
      361 agacggaaat cgtcacgaag cttccggacc acgccatagc gaaggctgcg gtggccgagc
      421 tgaaaaaatt cggcgcggga acccggttca tccagttcgg cggcgaacgg atgggcgtct
      481 acttcatcga aaagggagcg tcgcagaggc cctcgaaggt catctacgac agaaaaaata
      541 cctcgatttc ggctgtgcgc ccgggagagt tcgactggga gactattttc gagggagctt
      601 cggcctttct cttctccggc atcaccgcgg cgctgagcga cacgatgccc gacgtgctgg
      661 ccgaggcgtg cgaaaaggcg aaggaaaaag gcgtcaagat cttctgcgat ctgaactaca
      721 gaaagaacct ctggaccccc gagaaggcgc agatcacgat gaagcgcctt gtacagtacg
      781 tcgacgtgct cgtcggcaac gaagaagaca ccgagaagat gctgggcata aaggccggcg
      841 aatccgacgt gcacaagggc gagctggaca cggcgtcgta cgtggacgtc gcgaagcagc
      901 ttcagcggga gtacggcttc gaggccgtgg ggaccacgct ccgcaagtcg atatccgcct
      961 cggacaacaa gtggtccggg atgctctaca cgaacggaac ggcctatctc tccagagagt
     1021 acgacatccg gatcgtggac agggtgggcg gaggcgactc gttcacgtcc ggcctcatgt
     1081 acgccatgct caacggctac gacagcagga aggccctcga attcgctgtg gccgcctctt
     1141 gcctgaaaca cagtatcgaa ttggatttca acctcgtctc ggtggaggag atcgtcgccc
     1201 ttatggaagg cgacggctcc ggacgagtgc agcgatgaaa aaatccgcaa cgaagcggtc
     1261 cgccgggaga gaacccggga agggagatgc gcgatgaaga agctgttcgg
//
LOCUS       DAOSWW010000878         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_94944_length_1310_cov_1.733865, whole genome shotgun sequence.
ACCESSION   DAOSWW010000878 DAOSWW010000000
VERSION     DAOSWW010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94944_length_1310_cov_1.733865"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PK442_13865"
     CDS             <1..712
                     /locus_tag="PK442_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOO88786.1"
                     /translation="FVVMADPEYYPTLRKKHGAEEARKILLGGVENPLVQQIRDTFCK
                     YMAPLRNARREGDYLVEQWKSVGDVRSLMGADASEKMLYDQETKVWDAHHVHIVCHGY
                     DRNSIPDLQPGLALSPVADTQNDSFAQMGELASLRWRSDLVVLSACDTGLGDLFIGDG
                     MVGLNTVFLAGGAKGMLISRWRVPDESAPGFMNVFYERIVAGEMPVDALASAKRELKK
                     TYSEASHWAVFKYVGIPW"
     gene            769..>1310
                     /locus_tag="PK442_13870"
     CDS             769..>1310
                     /locus_tag="PK442_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO88787.1"
                     /translation="MKKYKMTCALLVLALLFGAAGAACSSELLRERAKNLYSVQDYRG
                     ALALFDRLLGEIPGDGEALDYSAWCLRYLGDWKSAEERFFQALDAPSGALTSWIHVGL
                     GETYLGAGDERKAVASFQKAIESAPGDDELFLRSQKGIAWAAAFLGEREEYGRALALI
                     REKDEKFAEEVLGDTGAILE"
BASE COUNT          279 a          338 c          421 g          272 t
ORIGIN      
        1 gtttgtcgtg atggcagacc cggagtacta tccgacgctt cgaaagaaac acggagcgga
       61 agaagcgcga aaaatccttc tcggcggcgt cgagaacccc ttagtgcagc agatacggga
      121 tacgttctgc aaatacatgg ccccgctccg gaacgcgcgg cgggaaggcg actaccttgt
      181 cgaacaatgg aagagcgtcg gagacgtacg ttccctgatg ggtgcggacg cctcggagaa
      241 gatgctctac gatcaggaga cgaaagtatg ggacgcgcac catgtgcata tcgtgtgcca
      301 cggctacgac cggaacagca tcccggatct tcagcccggg cttgctctct ccccggttgc
      361 ggacacccag aacgattcgt ttgcgcagat gggtgaactc gcctcgctga gatggcgttc
      421 cgatctcgtc gtcctctccg cctgcgacac cggcctgggc gatctcttca tcggcgacgg
      481 gatggtcggt ctgaacactg tctttcttgc cggcggcgcg aagggaatgc tcatctccag
      541 atggcgggtg ccggatgaga gcgctcccgg tttcatgaat gttttctatg aacgcattgt
      601 cgcgggggag atgccggttg acgcgctcgc ttcagcaaaa cgggaattga agaaaacgta
      661 cagcgaagcg tcgcactggg cggtatttaa atatgtgggt atcccatggt gaagttttaa
      721 aaaccgcttt tcggagagcg gtattttcct gcaagggggc tcaatatcgt gaaaaaatac
      781 aaaatgacgt gtgcacttct tgttctggca ctcctgttcg gtgcagccgg agctgcatgt
      841 tcttcggagc ttttgcgcga acgggcgaaa aatctgtata gcgtgcagga ctaccgggga
      901 gccctcgcgc tcttcgaccg gttgcttgga gagattccgg gcgacggcga ggcgcttgac
      961 tattcagcgt ggtgcctgcg ttacctcggc gactggaaga gcgcggaaga acgtttcttc
     1021 caggctcttg acgctccgtc aggagcgctg acgagctgga ttcacgtggg cctcggcgaa
     1081 acatacctcg gcgcgggcga tgaacgcaaa gcggttgcat ccttccagaa ggcgatagag
     1141 agcgctccgg gcgacgatga gctttttctt cgttcgcaga aggggattgc ctgggccgcg
     1201 gcatttctcg gagaacggga ggaatacggg cgggcgctcg ctctgatcag ggaaaaagac
     1261 gagaagttcg cggaggaagt tctgggcgac acgggagcaa tcctagagga
//
LOCUS       DAOSWW010000879         1304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_95578_length_1304_cov_3.541233, whole genome shotgun sequence.
ACCESSION   DAOSWW010000879 DAOSWW010000000
VERSION     DAOSWW010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95578_length_1304_cov_3.541233"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1061
                     /locus_tag="PK442_13875"
     CDS             198..1061
                     /locus_tag="PK442_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO88788.1"
                     /translation="MQLFIEQLLNGAAMGAIYALITLGLALVYGVLRILHVAHAGVYA
                     AGAYAGLYVYSHTGSLLLAVLGAMIFCALLGVLIERLIYTPLLKFPPFVPLIGSIAVF
                     LALEEIFRLVGGPYILSFPAELPFSGITVSGVVISPAIISIYAVSASVLLLLWFIVTK
                     TEFGLAMRATSQDREVAGAMGIDGAFTISMTFALGSAVAAVAGILVGIYFNQVYPTMG
                     AVPAYKCLALIVVGGLGSLPGAVLASLLLGVGETLLIGYANIPLPRDSLAFIAMIAVL
                     LWRPGGLMGNR"
     gene            1075..>1304
                     /locus_tag="PK442_13880"
     CDS             1075..>1304
                     /locus_tag="PK442_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO88789.1"
                     /translation="MSDYSITILNFIAIQAIVACGLNVIVGYAGQISLGHAAFFGIGA
                     YSSALLTTKAGLTFWAALPLVVLITAAIGLVLG"
BASE COUNT          178 a          434 c          408 g          284 t
ORIGIN      
        1 ggaaccggct gaaaaccgcg ttttttcgac gtcccgaacg cggagagtgc gtgtatacac
       61 taccggggct tttctgaaac cgccccccgt gtgctagaat tattttcgtt ccgctgagag
      121 aaacttcccg gcgcgctccg cgcggaagct tctttttcgg aacgcaatcc ggagttttct
      181 ggaggttggt atccgtcatg cagctcttca tcgagcaact tctgaacggg gcggcgatgg
      241 gggcgatcta tgccctgatc acgctcgggc tggccctcgt ctacggcgtc ctgcgcattc
      301 tccatgtggc gcacgcaggg gtgtacgccg ccggggccta cgccggcctg tacgtctact
      361 cgcacaccgg cagtctgctt ctggccgtcc tcggcgcgat gatcttctgc gcgctcctcg
      421 gcgtgctcat cgaacggctg atctacacgc cgctgctgaa gttccccccg ttcgtcccgc
      481 tcatcgggag catcgccgtt ttccttgcgc tggaggagat tttccgcctc gtcggcggcc
      541 cgtatatcct ctcctttccg gcggagctgc ccttctccgg gatcaccgtg agcggcgtgg
      601 tgatttcccc ggcgatcatt tccatctacg ccgtgagcgc ctccgtcctg ctgcttctct
      661 ggttcatcgt gacgaagacc gagttcggcc tcgccatgcg ggcgacgtcg caggacaggg
      721 aggtcgcggg agcgatgggg atcgacggcg cgttcaccat cagcatgacc ttcgccctcg
      781 gctccgccgt ggcggcggtc gcgggcattc tcgtcggcat ctacttcaac caggtctacc
      841 ccacaatggg ggcggttccg gcgtacaagt gcctcgccct catcgtcgtc ggcgggctcg
      901 gctcgctgcc gggggcggtg ctcgcctcgc tgctgctcgg cgtgggcgag acgctgctga
      961 tcggctacgc gaacatcccg ctgccgaggg attccctggc gttcatcgcc atgatcgccg
     1021 tgctgctctg gaggccgggc ggcctcatgg gcaacagata ggggagggaa cgacgtgagc
     1081 gactacagca tcaccattct gaacttcatc gcgatacagg ccatcgtcgc ctgcggcctc
     1141 aacgtcatcg tggggtacgc cgggcagatt tcgctggggc acgccgcctt cttcggtatc
     1201 ggcgcgtact cttcggcgct tctcaccacg aaggcggggc tcaccttctg ggcggcgctg
     1261 ccgctcgtgg tgctcatcac cgcggccatc gggctcgtcc tcgg
//
LOCUS       DAOSWW010000880         1302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_95868_length_1302_cov_2.156375, whole genome shotgun sequence.
ACCESSION   DAOSWW010000880 DAOSWW010000000
VERSION     DAOSWW010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95868_length_1302_cov_2.156375"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..>1302)
                     /locus_tag="PK442_13885"
     CDS             complement(237..>1302)
                     /locus_tag="PK442_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88790.1"
                     /translation="SMTYDGWLVVTFSNGVSVIKRDFDLSSKVFYPFGEDEYVSNSMA
                     VDENNGMYIASNKLMRKLVWTGTAISDREEDGAWASLYDVADVLPPIIKFDNGTGSTP
                     TLMGFGDDPDKLVVITDGAKHMKLTAFWRDAIPEGFVQKPGAASRRIAGQIQATFGLP
                     DPLPEWLQSEQSVVVSGYGAFVVNNMPATVDPELQATNKICAVALMGPAYPGPLGVER
                     FEWNPATDEWRSVWNRPDVSSTSMVPVHSQSGSMALVNGYYTETGWEVTGLDWNTGNT
                     VHRTVFGHKNLGNGAYAIIQYLSDEYLLFNSIVGPYLAGYKETSSSGGGCSVAGTAGF
                     AAPALLLIVPLLLAVKGKKW"
BASE COUNT          245 a          408 c          384 g          265 t
ORIGIN      
        1 agacgcttga tcccctctct ctttcgggga gggagcatcg ccttggtatc tggaaacccc
       61 tcttcgtcga cagagccgat cagcgacacc ggcgccttgt cgatcaagtt ccccaccgtc
      121 ttttcaggat ctcgcactgc aaaggcctcc ttaaaaccag cttctcttgc gaacaacgag
      181 aaaaaaagtc cgtcgcatcg cgtcgtgaat gcgcgatgcg acggtatact acagtcttac
      241 cacttttttc ccttgacggc aaggagcagc gggacgatca gcagcagcgc gggggccgcg
      301 aatccggccg tccctgccac ggagcacccg cctccggaag acgacgtctc tttatatccg
      361 gcgagatacg gaccgacgat ggagttgaag aggagatatt catccgacag gtactggata
      421 atagcgtacg cgccgttccc caggttcttg tgtccgaaga ctgtccggtg aaccgtattc
      481 ccggtgttcc agtcgagccc tgtaacctcc cagccggttt ccgtgtagta cccgttgacc
      541 agagccattg agcctgactg gctatgcaca ggcaccatgc tggtcgagct gacgtccggg
      601 cggttccaga cggaacgcca ttcgtcggtc gcaggattcc actcgaagcg ttccacgccg
      661 agagggccgg ggtacgccgg tcccataagg gcgacggcgc agatcttgtt cgtcgcctga
      721 agttccgggt cgaccgtcgc cggcatgttg ttcacgacga aggccccgta ccctgagacc
      781 accacggact gctccgactg gagccactcg ggaagcggat ccggcaggcc gaacgtcgcc
      841 tgaatctgcc ccgcgatgcg ccgggaggcc gcgcccggct tctggacgaa tccttcgggg
      901 atggcgtcgc gccagaatgc cgtcagtttc atgtgtttcg cgccgtcggt gatgaccacc
      961 agcttgtccg ggtcgtcgcc gaatcccatg agcgtcggcg tggagcccgt gccgttgtcg
     1021 aacttgatga tcggcggcaa aacgtcggcc acgtcgtaga ggctggccca cgcgccgtcc
     1081 tcctctctgt cggagatcgc cgttccggtc cagacgagct tgcgcatcag cttgttgctg
     1141 gcgatgtaca tcccgttgtt ctcgtccacg gccatggagt tgctcacgta ctcatcctct
     1201 ccgaagggat agaatacctt ggaggagagg tcgaaatcgc gcttgatgac cgacacgccg
     1261 ttgctgaacg tcaccacgag ccagccgtcg taggtcatag ag
//
LOCUS       DAOSWW010000881         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_95934_length_1301_cov_3.692616, whole genome shotgun sequence.
ACCESSION   DAOSWW010000881 DAOSWW010000000
VERSION     DAOSWW010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95934_length_1301_cov_3.692616"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..736
                     /locus_tag="PK442_13890"
     CDS             236..736
                     /locus_tag="PK442_13890"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOO88791.1"
                     /translation="MIDFTKGYSFNEYLEKGYEEERDRQRRACSRAVFSPAFEEAARN
                     VNKPLKLAAFAEIYCPDSVVTMSFVHRIAELNPKIELAVFPRSPFEKELEALTGSSRI
                     PTLLFFDEKMNLAGSYVELPKALKEDMRDLDAGEKSALTLDYRRGKYNDILQAELTEI
                     LSLFAS"
     gene            815..>1301
                     /locus_tag="PK442_13895"
     CDS             815..>1301
                     /locus_tag="PK442_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOO88792.1"
                     /translation="MPLSSIFLLFLRIGAFTFGGGLVMLGFIEKELRSTARLSSEEIA
                     DMMVLATAFPGPVAANMAFLAGKRLAGIAGSFCALLGTVIPPFLTILLLSKAVIHFMN
                     EPWLAAFFLGAACAVVVIIGRVVYSMTRVSCGGRWKDIAVFAAVGILLISTEVHPLFA
                     LA"
BASE COUNT          275 a          308 c          363 g          355 t
ORIGIN      
        1 ctttctccct gttctccttt ttttgcaagc caaaggtgcg ttcgtattcc atatatacga
       61 ccgtgagcat cgtatgcggg caatagcatg aaaacgagtc catcgcagga tatctctttc
      121 agtcctgcgg tatcggttca ggttttgagt cgcaactcgt ggaaataccc tttgagaaaa
      181 acgtccgcga agggtaatat agactgcata ctgcaaagaa tgcggggtgg agcgtgtgat
      241 tgattttaca aaggggtatt ctttcaatga gtaccttgaa aaagggtacg aagaagagcg
      301 ggacagacag cggcgcgcat gttcgcgtgc cgtcttttct ccggcattcg aagaggcggc
      361 tcggaatgtg aacaaacctc tcaaattggc tgcgtttgcc gaaatttact gcccggactc
      421 ggttgtgaca atgtcttttg tccaccggat agcggagctg aacccaaaga ttgaacttgc
      481 ggtttttccg cgatctccgt ttgaaaagga gctggaagcg ctgacaggtt cctccaggat
      541 tcctacgctt ctctttttcg acgaaaaaat gaaccttgca ggcagctacg tagagcttcc
      601 aaaagcgttg aaagaggata tgcgcgatct cgacgccggg gagaaaagcg ctcttactct
      661 cgattacagg cgaggaaagt ataacgacat actccaggcc gagcttacgg agatactctc
      721 actgtttgct tcatgacgtt ctccgcgtac gaaggatttg gagcgcgttc ggggcgtctt
      781 ttttgagtgg ccgggtgttt tgggaggtcg atgaatgccg ctctcttcaa tttttttatt
      841 gtttcttcga atcggcgcct ttacctttgg cggcgggttg gtcatgctgg ggtttatcga
      901 aaaagagctg cgatcaactg cgcggttgtc gtccgaggaa atagcggaca tgatggtact
      961 tgctacggcg tttcccggtc cggttgccgc caatatggca ttcctggcag ggaaacgcct
     1021 tgctggcatt gccggttcgt tttgcgctct attaggcacg gtcatccctc cgtttctgac
     1081 aatcctgctg ttgtcgaaag cggtgatcca tttcatgaac gaaccctggc ttgcagcttt
     1141 tttccttggc gcggcctgcg cagtcgtcgt cattatcgga agggtcgtct acagtatgac
     1201 tcgcgtctcc tgcggcggaa ggtggaaaga tatagcggtg ttcgcggcgg tggggattct
     1261 gcttatcagc actgaagtac acccgctttt tgccctcgcg g
//
LOCUS       DAOSWW010000882         1299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_96205_length_1299_cov_3.446141, whole genome shotgun sequence.
ACCESSION   DAOSWW010000882 DAOSWW010000000
VERSION     DAOSWW010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96205_length_1299_cov_3.446141"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PK442_13900"
     CDS             complement(<1..247)
                     /locus_tag="PK442_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO88793.1"
                     /translation="MLDESRAVLVNAAVGSGKTTVLIAKALYEHAARGVPLREMAVLT
                     FTNKAADEIRERMRAADPDAGDEETAWFGTFHSVALRL"
     gene            complement(311..>1299)
                     /locus_tag="PK442_13905"
     CDS             complement(311..>1299)
                     /locus_tag="PK442_13905"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOO88794.1"
                     /translation="TVSCDTTEVRKLQELKRSAAGRTTRRFGDVRVSYTTLGFRRQQF
                     HNHQNLGYEALERPLSKTFDTEGFWIRLPDEVRTLFLALTPQEENRPPMQFWKTYAEG
                     LGFALLNATMMATMTTHDDIGSALLADTDGEGGGMSVCVFDMYAGGLGFADKGYDRVS
                     EIIANAALMVEGCACTDGCAACVGDYHLDKAVILWGLKSLLEELPAPKTLRRRTPRAS
                     AQQPEKPFRMETLADEWGAFREALLSSGDALGHFLASVPAARTDGSRLVLSLRHEFSA
                     EWLSEATYREQLEALIRRFAHVPEDFSVGYEILNGDASIASKLGKMRKDLAR"
BASE COUNT          182 a          474 c          410 g          233 t
ORIGIN      
        1 gcaggcgcag ggccacgctg tggaacgtgc cgaaccaggc cgtctcctcg tctccggcgt
       61 ccgggtcggc ggcgcgcatc cgctccctga tctcgtccgc cgccttgttg gtgaaggtga
      121 gcaccgccat ctcccgcagc ggcacgccgc gcgcagcgtg ctcgtacagc gccttggcga
      181 tgagcacggt ggtctttccg ctgccgacgg cggcgttcac caggacggcc cggctctcgt
      241 cgagcacggc ccgccgctgc tgttcgttca gtctttgaag tatctcgccg ccgtcgatag
      301 ccgctccccc ctatcgcgca aggtctttgc gcatcttccc gagcttggac gcgatcgagg
      361 cgtcgccgtt caggatttcg taccccacgg aaaagtcctc cggaacgtgc gcaaacctcc
      421 ggatcagggc ttcgagctgc tcccggtacg tcgcctccga cagccactcc gcggagaact
      481 cgtgccgcag cgacaggacg agcctgcttc cgtcggtccg cgccgccggc acgctcgcca
      541 gaaaatgccc gagggcgtct cccgaggaga ggagcgcctc ccggaacgcg ccccactcgt
      601 ccgccagcgt ctccatgcgg aacggcttct ccggctgctg cgcggaggcg cgcggcgttc
      661 tcctcctgag tgtcttcggc gcggggagtt cttcgagcag gctcttcagc ccccagagga
      721 tcaccgcctt gtccagatgg tagtcgccca cgcacgcggc gcagccgtcc gtgcacgcgc
      781 acccctcgac catcagcgcc gcgttggcga tgatctccga tacgcggtcg taccccttgt
      841 ccgcgaagcc cagtcctccg gcgtacatgt cgaaaacgca gaccgacatg ccgcctccct
      901 ctccgtcggt gtccgccagc agcgcgctgc cgatgtcgtc gtgcgtcgtc atcgtcgcca
      961 tcatcgtcgc gttgagcagg gcgaacccca gcccctcggc gtacgtcttc cagaactgca
     1021 ttggcgggcg gttctcctcc tgcggggtga gcgccaggaa gagggtgcgg acctcgtcgg
     1081 gaaggcggat ccagaacccc tcggtatcga acgtcttgct caacgggcgc tccagcgcct
     1141 cgtaccccag gttctgatgg ttgtggaact gctggcggcg gaagccgagc gtggtgtacg
     1201 agacccgcac gtcgccgaag cgccgcgtgg ttctgcccgc cgcgctccgc ttcagctcct
     1261 ggagcttgcg cacctcggtc gtgtcgcagg agaccgtgt
//
LOCUS       DAOSWW010000883         1299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_96209_length_1299_cov_3.374598, whole genome shotgun sequence.
ACCESSION   DAOSWW010000883 DAOSWW010000000
VERSION     DAOSWW010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96209_length_1299_cov_3.374598"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PK442_13910"
     CDS             <1..642
                     /locus_tag="PK442_13910"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HOO88795.1"
                     /translation="PSVFSRSGYRAGISFQLSEGFSPRPRISLGPELPVGVPALSEPL
                     EVRLLSFSADELIRWDRALPPGFFFTGACVVTGEPGSAEAKSLNKWCEASSCILALRE
                     EPAGASPLESFLQEMKDLGDVLAFERAEPLGFRCVLENPAQRSPGFLVKALQKRGIIE
                     GWPDIFILREAVGRLDMAEESGTAHVLPLTHPLSEEFEQALEKVATGESVFSE"
     gene            864..>1299
                     /locus_tag="PK442_13915"
     CDS             864..>1299
                     /locus_tag="PK442_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HOO88796.1"
                     /translation="MRGGSVAGNADRKIIANCIDPEETRVAIIEDGRLADLFVERMWE
                     RQKAGEIYKARVESVLPGIHASFVNLGDGRNAFLYLNDARGLDLQPNKELLVQVTKTA
                     RKNKGARVTSRISLPGRYVVLVPGGQESGVSKRIVDENERKRL"
BASE COUNT          262 a          358 c          432 g          247 t
ORIGIN      
        1 ccttcggtct tctccagaag cggataccgc gctggaatct ccttccagct ttcggagggg
       61 ttttccccgc ggccgcgcat cagcctcggc ccggagctcc ctgtgggtgt tcccgcgctg
      121 agcgagccgc tggaagtccg tttgctctcc ttttcggcgg atgaacttat caggtgggac
      181 agagcgcttc ccccgggttt tttcttcaca ggggcgtgcg ttgtgaccgg agaacccggg
      241 tctgcggagg cgaagtcgct gaacaagtgg tgcgaggctt cctcgtgcat cctcgctctg
      301 cgggaggagc ctgccggcgc atcgccgctg gaatccttcc tgcaggagat gaaggacctg
      361 ggggacgtac ttgctttcga gcgggcggag cccttgggct tcaggtgcgt tctggagaac
      421 cccgcgcaaa ggagtcccgg cttcctggtg aaggcgctcc aaaaaagagg tatcatagag
      481 gggtggcccg atatcttcat cctgagggaa gcggtggggc ggcttgacat ggcggaagag
      541 agcggaacgg cgcacgtgct tccgctgacg catcctctct ccgaggagtt cgagcaggcg
      601 cttgaaaagg ttgcaaccgg ggagtccgta ttttcagagt gaggttcccc ggagtataga
      661 taaagaaagc ggcgggaatt ttctccttct catgaaaaag agaaggagat tcgtcctgtt
      721 gtacatgttt gtccgaaaag aggagggagt tgcgtttgtc agagaatgcg gaggccagaa
      781 gcgaacgcgc ggaatcgcgc gacgctgtga taccccgtac cgaagcgagg gaggacgtcg
      841 atccgcagga gggaaggccc ggtttgcggg gagggtccgt cgccggcaac gccgacagga
      901 agattatcgc gaactgcatc gaccccgaag aaacgcgggt tgccatcatc gaggacggcc
      961 gtttagccga cctgttcgtc gagcgcatgt gggagcgtca gaaggcggga gagatctaca
     1021 aggccagggt tgagagcgtg ctccccggca tccacgcttc cttcgtcaac cttggcgacg
     1081 ggaggaacgc ctttctgtac ctcaacgacg ccagggggct cgatctccaa ccgaacaaag
     1141 agcttctcgt ccaggtgaca aagaccgcca ggaagaacaa gggagcgcgt gtgacctcca
     1201 gaatctccct ccccggtcgc tacgtcgtgc tcgttcccgg cggacaggag tccggagtct
     1261 ccaagcggat cgtcgacgag aacgagcgaa aacgcctga
//
LOCUS       DAOSWW010000884         1297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_96463_length_1297_cov_3.322866, whole genome shotgun sequence.
ACCESSION   DAOSWW010000884 DAOSWW010000000
VERSION     DAOSWW010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96463_length_1297_cov_3.322866"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1167)
                     /locus_tag="PK442_13920"
     CDS             complement(58..1167)
                     /locus_tag="PK442_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOO88797.1"
                     /translation="MNATNDGAMETPACPVCDLVSLLYTRNVMVYLYSSLAFVPRRVI
                     EGLAASGYSPQERGMYLEIEAERFSDVIEALGRSCGLTDPEKGATVVCFTERGRPFGL
                     DSLAHVKPLSQWEDILGAGDLLDVLRNGRIVTHFQPIVDYAAKSVYACECLSRGVRSD
                     GERINPAELFGKAKKAGLIFNLDKLCRQSAIVSGASLCGSGRLFINFIPTAIYNPEHC
                     LQTTTALVEEYGVAPEKIVFEVVESESVTDLRHLQGILDYYRERGFRTALDDVGSGYS
                     SLNRLIGLRPDYVKLDIGLVRDIHKEPMKQHVFEAIETICRRSGIEMLAEGIEHAEEV
                     AFFHNAGVRLMQGYFFAKPAEVPQPVSFDDRPSSS"
BASE COUNT          267 a          396 c          374 g          260 t
ORIGIN      
        1 gacacagagg ataccgcatc cggaacgcaa ccgcctgacc gtcgttgcgc atttttatca
       61 ggatgaagaa ggtcgatcgt caaaagagac aggctgcgga acctccgcgg gtttcgcgaa
      121 gaagtaccct tgcatcaggc gcactccggc attgtggaag aacgcaacct cttcggcgtg
      181 ctcgatgcct tccgcgagca tctcgatacc gcttctgcgg caaatcgttt cgatggcctc
      241 gaagacatgc tgtttcatcg gctccttgtg gatgtcccgg acgagtccga tgtcgagttt
      301 cacgtagtcg gggcgcagac cgataaggcg attcagcgac gagtagccgc tgccgacgtc
      361 gtcgagcgcc gtcctgaatc ctctttcacg gtagtagtcc aagataccct gaaggtggcg
      421 cagatcggtg acgctctccg attcgacgac ctcgaagacg atcttctccg gcgccacgcc
      481 gtactcctcg actagggccg tcgtggtctg cagacagtgt tccggattat agatcgccgt
      541 ggggatgaag ttgatgaaga gccttcctga accgcagagc gacgctccgg aaacgatggc
      601 ggactgccgg cagagtttgt ccagattgaa tatcaatccg gcttttttcg ccttgccgaa
      661 gagctcggcc ggattgattc gttcgccgtc gctccgcacg cctcgggaaa gacattcgca
      721 ggcgtacacg ctcttcgccg cataatcgac gatcggctga aagtgggtga caatccgccc
      781 gttgcgcagc acgtcgagca gatctcccgc gcccaggata tcctcccact gcgaaagagg
      841 ttttacatgc gccagcgagt ccaatccgaa cggccgtccg cgctcggtga aacagacgac
      901 cgtggctccc ttctccgggt cggtcaagcc gcaggatctc ccgagggcct ctatgacgtc
      961 tgaaaagcgc tccgcctcta tctccaggta catacctcgc tcctgcggag aataccccga
     1021 ggcggcgagc ccttcgatca ctcttcgggg aacgaaggcg agcgaactgt agaggtatac
     1081 catgacgttc ctcgtataga gcaaagagac gagatcgcac accgggcatg ccggagtttc
     1141 catcgcgccg tcgttcgtcg cgttcactgc gccctctcct ctcccaggtc ctcgtacgct
     1201 tccgggagga agcgcggaga gcagacgcag ataaagacca gatcatccgt tccggtgttg
     1261 gaaatgcgct gccgcaccag cggcgggatg atcaccg
//
LOCUS       DAOSWW010000885         1295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_96738_length_1295_cov_2.823387, whole genome shotgun sequence.
ACCESSION   DAOSWW010000885 DAOSWW010000000
VERSION     DAOSWW010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96738_length_1295_cov_2.823387"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..>1295)
                     /locus_tag="PK442_13925"
     CDS             complement(335..>1295)
                     /locus_tag="PK442_13925"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOO88798.1"
                     /translation="TMTHFGYIKRLSPDIYRAQNRGGRGVIGQGTREEDFVERLFVTS
                     THSYIMFFTNFGRAFRIKCYAIPEEGRNAKGMPIVNLLQLGEGEKVTAMFPVAAEGAE
                     GYLVMATKQGVIKKTPLSALSNIRRGGLIVQSLREGDALIAVEYSTGDDEFIIASKKG
                     KCVRFAEDDVRPMGRTAMGVRSINLEDDDAVVDMIKIIKGADVLTVTELGMGKRTPEE
                     QYPLHHRGGKGVLAAQLTEKTGDLACLKMTTEGEDLMLIRDDGTIIRMPLDQISVFSR
                     NTQGVHLMRVDEGTRVVSVEIVPHQEDDGEEAPAAPAAPEEQN"
BASE COUNT          206 a          436 c          392 g          261 t
ORIGIN      
        1 caccgccatg atattgcttg cgtatacgga gtttgcccag tcgtagaaga tccagctttt
       61 ttcagctttt gtgaaccgtt tgccgacgtc tttccgctcc atcgaaagcc accccttctt
      121 tccctgatcc cgttagatgc agtatagcaa aaaaccgccc cgggaacaag agacgctctc
      181 gcgctttccg tcgcccgcgg tgttcgcgcc ggtcgcggcg gcaccgctct ctgcggaaga
      241 acggacgcgt gaaaagagcc cgggaccggc ggcaaagccg ccggtcccgg gccggtatgc
      301 gctgcgttcg gaggatttga aaggaatcct cccgtcagtt ctgttcttcc ggcgcggccg
      361 gcgcggcggg cgcctcttcg ccgtcgtcct cctgatgcgg cacgatctcg accgaaacca
      421 cgcgggtccc ttcgtccacg cgcatgagat gcacgccctg cgtgttccgg gagaaaacgc
      481 tgatctgatc gagcggcatg cggatgatgg tcccgtcgtc gcggatcagc atgagatcct
      541 cgccctcggt cgtcatcttg aggcacgcga gatcacccgt cttctcggtg agctgcgcgg
      601 ccagcacgcc cttcccgccg cggtgatgca gcggatactg ctcctccggc gtgcgtttgc
      661 ccatgccgag ctcggtcacg gtgagcacgt ccgcgccctt gatgatcttg atcatatcga
      721 ccacggcgtc gtcgtcctcc agattgatcg aacgcacgcc catggcggtg cggcccatcg
      781 ggcggacgtc gtcctccgca aagcggacgc acttgccctt cttcgaggcg atgatgaact
      841 cgtcgtcgcc cgtggagtac tccacggcga tgagcgcgtc gccctcgcgc aggctctgca
      901 cgatcaggcc gcccctgcgg atgttgctga gcgcagacaa cggcgtcttc ttgatcacgc
      961 cctgtttcgt ggccatcacg aggtacccct cggcgccctc ggcggcgacg gggaacatcg
     1021 cggtcacctt ttcgccctcg ccgagctgca ggaggttcac gatcggcatg cccttggcgt
     1081 tgcggccctc ctcggggatg gcgtagcact tgatgcggaa cgcccttccg aagttcgtaa
     1141 agaacatgat ataggaatgc gtgctcgtca cgaacaggcg ctcgacgaag tcctcctcgc
     1201 gcgtgccctg cccgatcacg ccgcgcccgc cgcggttctg cgcgcggtag atgtccggcg
     1261 agagccgctt gatgtacccg aagtgcgtca tcgtg
//
LOCUS       DAOSWW010000886         1294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_96840_length_1294_cov_4.079096, whole genome shotgun sequence.
ACCESSION   DAOSWW010000886 DAOSWW010000000
VERSION     DAOSWW010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96840_length_1294_cov_4.079096"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PK442_13930"
     CDS             complement(<1..1113)
                     /locus_tag="PK442_13930"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO88799.1"
                     /translation="MPGSELFDHLEIEAVADVLKRKVIHRYAYHEVRDGNYRVDEFEA
                     KVAELSGAKHCLAVSSGTAALYVPFKAMGICPGDEIITSSFTFIATVEAILECGAVPV
                     LGDVDESLNLSPDSAEDLITERTKAIMPVHMFGAAADMDSFRSLGAKYGIPVVEDACQ
                     AMGGTYKGKALGTMGLWGSYSLDPNKLLTVGEGGLIVTDDDELYSKMEYYHDHGHIHS
                     KILDRGAEGKACLGFNFRMSELQGALGLVQAAKMQQAISMTRKTKSTILDAVKKTGLE
                     LRDLPDREGEIATQIVFLLPTPEKASLFQKTVKEAGAGCSILSGNTWHYAKHWTTLKE
                     GAMYSRTRCPYDCPYVEEMPLYRPLEWERTEKILSRA"
     gene            complement(1115..>1294)
                     /locus_tag="PK442_13935"
     CDS             complement(1115..>1294)
                     /locus_tag="PK442_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent alcohol dehydrogenase"
                     /protein_id="HOO88800.1"
                     /translation="HQLVADINLPSTFPELDPRITEDHFATMAEKAMGVARPMANNPR
                     TMNTDLCMEIYKEAM"
BASE COUNT          231 a          368 c          376 g          319 t
ORIGIN      
        1 ggctctgctc aggatctttt cggtccgttc ccactcaagc ggccgataga gcggcatctc
       61 ttccacatag ggacagtcgt aggggcagcg tgttctgctg tacatcgcgc cctccttgag
      121 cgtcgtccag tgtttcgcgt agtgccaggt gttgcccgac aggatagaac accccgctcc
      181 ggcttctttt acggtttttt ggaagaggga ggccttttcc ggagtcggaa ggaggaagac
      241 aatctgcgtg gctatttcgc cttcacggtc cggaagatca cggagttcga gccccgtctt
      301 tttcacggcg tccagaatcg tcgatttcgt ctttctggtc atcgagattg cctgctgcat
      361 ttttgcagcc tgaacgaggc caagggcgcc ttgcaattcg ctcatgcgga agttgaatcc
      421 aaggcaagcc tttccttcgg cccctctgtc caggattttg ctgtggatgt gcccgtggtc
      481 gtggtagtac tccattttgc tgtagagctc gtcgtcgtcg gtcacgatca atccgccttc
      541 gccgacagtg aggagtttgt tcgggtccag actgtaggaa ccccagagtc ccatagtccc
      601 gagcgccttc cctttatacg tccctcccat tgcctggcag gcgtcctcga cgacggggat
      661 gccgtatttg gctcccagtg agcggaacga gtccatgtcc gccgcggcgc cgaacatgtg
      721 caccggcatg atcgctttcg tgcgctccgt tatgaggtcc tccgccgaat cggggctgag
      781 gttcaggctt tcgtcgacgt ctccgaggac cggaacggcc ccgcactcca ggatggcttc
      841 aaccgtggcg ataaaagtga acgaggatgt gatgatctcg tctccggggc agatccccat
      901 cgccttgaac ggaacgtaaa gcgcagccgt accgctgctc acggcgagac agtgcttcgc
      961 tccggagagt tccgcgactt ttgcctcgaa ttcgtccacc ctgtagtttc cgtcccgtac
     1021 ctcatgataa gcgtagcggt gaatgacttt gcgcttgagg acgtcggcga cggcctctat
     1081 ttctagatga tcgaagagtt cggaacccgg catgttacat cgcctcctta tagatttcca
     1141 tgcaaagatc ggtattcatc gtccgggggt tgtttgccat cggacgcgcg acgcccatcg
     1201 ccttctcggc catcgtcgcg aagtggtctt cagtgatacg aggatcgagt tcgggaaaag
     1261 ttgagggaag gttgatgtcg gcgacaagct ggtg
//
LOCUS       DAOSWW010000887         1294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_96868_length_1294_cov_2.919290, whole genome shotgun sequence.
ACCESSION   DAOSWW010000887 DAOSWW010000000
VERSION     DAOSWW010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96868_length_1294_cov_2.919290"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PK442_13940"
     CDS             complement(<1..472)
                     /locus_tag="PK442_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOO88801.1"
                     /translation="MLITVLYGGEGPEREVSLRSGAAVTSAFAERGRDVTGADLRRKE
                     DLFALLDEGNVEFAFIALHGGWGEDGTLQAALEMAGVPFSGSGHAACALAMDKTASKA
                     LFRWKGISVPKGIEVARGRCVRDVLDDPHFPPLLERSGKLVVKPCCSGSTVGVSI"
     gene            617..1051
                     /locus_tag="PK442_13945"
     CDS             617..1051
                     /locus_tag="PK442_13945"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOO88802.1"
                     /translation="MNIQWSDELAIGIGIIDDQHKKLIERIASFSHAVDGGDLHKVEE
                     TVNYLVGYAIQHFGAEELIMIRNCYDQFKEHRDEHSWFIKQVFDVHTSLVQKEECTQR
                     QIEDLRDMLYNWTLDHIMVKDKRIAQTLNSFGGPVRQGGGQA"
     gene            1120..>1294
                     /locus_tag="PK442_13950"
     CDS             1120..>1294
                     /locus_tag="PK442_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOO88803.1"
                     /translation="MSDFHLDVLREVSNIGAGNAATSLSTLLGKAVDMRVSRVLPMPF
                     NEIMEYVGGAERMI"
BASE COUNT          289 a          401 c          377 g          227 t
ORIGIN      
        1 ggatggagac cccgacggtg ctccccgaac agcacggctt gaccacgagt tttcccgagc
       61 gctccagaag cggcgggaaa tgaggatcgt ccagcacgtc ccggacgcag cgtccccgcg
      121 cgacctcgat ccccttcggt acggagatgc ccttccagcg aaagagcgcc ttgctcgccg
      181 tcttgtccat cgcaagggcg caggcggcgt gtccggagcc ggagaacgga accccggcca
      241 tctccagcgc cgcctggagc gtaccgtcct ctccccaccc gccgtggagg gcgatgaagg
      301 cgaactcgac gttcccttca tcgagaagcg cgaaaagatc ctccttgcgg cgaagatccg
      361 cacctgtcac atcgcgcccc cgctccgcga aggcggaagt cacggccgcg ccgcttctga
      421 gggagacctc cctctcgggg ccttctccac cgtagagaac ggttatcaac atgggaattc
      481 aatcctctcc ttaatatccg tattctggcc cattattata cacaaaccag tcttcttccg
      541 ctcttctttc tggtacaata cagcagacgg gtgttttccc gccaagtttc tatcgcccct
      601 gacgggagga atacacatga acatacagtg gagcgacgaa ctcgccatcg gcatcggcat
      661 catcgacgac cagcacaaga agctgatcga gcgcatcgcc tctttttcgc acgccgtgga
      721 cggcggcgat ttgcacaaag tcgaggagac ggtgaactat cttgtggggt acgcgattca
      781 gcacttcggg gcagaagagc tgatcatgat ccgcaactgc tacgaccagt tcaaagagca
      841 cagagacgag cacagctggt tcatcaagca ggttttcgac gtgcacacca gcctggtgca
      901 aaaagaagaa tgtacgcagc gacagataga agacctccgc gacatgctgt acaactggac
      961 ccttgatcat atcatggtga aggacaagcg catcgcgcag acgctcaaca gtttcggcgg
     1021 cccggtgcgc cagggagggg gccaggcatg acgggcgaac ggcgggaaag cggaaacgaa
     1081 gggtttcgct cgcccgaaac gggggacgcg ctgcaaagga tgagcgactt tcacctcgac
     1141 gtgctcaggg aggtcagcaa catcggcgcc ggaaacgccg ccacatctct ctccacgctg
     1201 ttggggaagg ccgtcgacat gcgcgtgtcg cgcgtcctcc cgatgccctt caacgaaatc
     1261 atggagtacg tcgggggcgc ggaacggatg atct
//
LOCUS       DAOSWW010000888         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_97006_length_1293_cov_2.693861, whole genome shotgun sequence.
ACCESSION   DAOSWW010000888 DAOSWW010000000
VERSION     DAOSWW010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97006_length_1293_cov_2.693861"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PK442_13955"
     CDS             complement(<1..934)
                     /locus_tag="PK442_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HOO88804.1"
                     /translation="MKRPWKAFSVFMIFALLLAGCCVVHRPPVAEATTPPDAQALKLL
                     PPIGGLTVQQMGSHAVVMQLTGASLSRPEITSNSPDLIVFSVKPAYLASGKWERQFEL
                     PLVQGVYAEQKGEAVVFNVRINGTLVLKDIQGVAPANRYILRFDATGHAAREREEPAL
                     TAPKPAQPTNYNDPFTRTTPVTFDLRDVELRDVFRMLGEYVGMNIVADPSVPNTLVTM
                     SLKGVPLNEALGYILRMYDMGYAIMGKTIIVGAPDNIAKTTGKQSTRAFKIAYADMKT
                     VGGLVQSLAGVSNIVLDERLRTLYVTSRPEQFPHV"
     gene            complement(946..>1293)
                     /locus_tag="PK442_13960"
     CDS             complement(946..>1293)
                     /locus_tag="PK442_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88805.1"
                     /translation="RQGSKGLVATVTEKNRRPFAVSRRSTKGAGGGAVGDIPLVEPLV
                     TESLPPIMFVRAIMVVGKESIAIMDINGVGNGIIVKTGYSFLEKKGRILRITPEKVTV
                     RWAGKNIDITPGF"
BASE COUNT          269 a          425 c          337 g          262 t
ORIGIN      
        1 ccacatgcgg gaactgctcc ggcctgctcg tcacgtagag cgtgcgcaaa cgctcgtcca
       61 acacgatatt ggagactccg gcaaggcttt gcactagccc cccgaccgtc ttcatatccg
      121 cgtaggcgat cttgaacgcc ctcgtcgact gttttccggt ggtcttggca atattgtcgg
      181 gcgcgccgac gatgatcgtc ttccccatga tggcgtaccc catgtcgtac atccgcagaa
      241 tatacccgag cgcctcgttc agaggaacgc ccttcagcga catggtcaca agcgtgttcg
      301 ggaccgacgg gtcggcgacg atgttcattc ccacgtattc cccaagcatt ctgaagacat
      361 cgcgcagctc cacgtcgcgc aggtcgaacg tcacaggagt cgtccgcgta aacgggtcgt
      421 tgtagttcgt cggctgggcg ggcttcggcg cggtaagcgc cggctcctcc cgttcgcggg
      481 cagcatgccc ggtggcgtca aaacggagta tataccggtt cgcaggggca acgccctgga
      541 tatccttcaa aacgagcgtt ccgttgattc gcacgttgaa gacgactgcc tctcccttct
      601 gctccgcata tacgccctgc accagcggaa gctcgaactg ccgctcccac tttccggacg
      661 ccagataggc cggtttgacg gaaaaaacga tcaaatccgg cgaattactc gtgatctccg
      721 gcctcgaaag agacgcgccc gtaagctgca taaccacggc atgcgacccc atctgctgca
      781 ctgtaagccc cccgatggga ggaagcagtt tcagcgcctg agcatccggg ggcgtcgtcg
      841 cttcggccac ggggggacga tgaacaacgc aacagccggc tagaagcaga gcaaaaatca
      901 taaaaacaga aaaagccttc catggtctct tcatcctgat tccccctaaa atcccggtgt
      961 aatatcgata ttcttgccgg cccaccggac cgttaccttc tccggcgtaa tcctgaggat
     1021 ccgtcccttc ttttcgagga acgaatagcc cgtctttacg ataataccgt tcccgacgcc
     1081 gtttatatcc ataatagcga tggattcctt ccccaccacc atgatcgccc gaacgaacat
     1141 gatcggcgga agcgactccg tcacaagcgg ctcaacaagc gggatgtctc cgaccgctcc
     1201 gccgcctgcc ccctttgtcg acctgcggga aaccgcgaac ggacggcggt tcttctccgt
     1261 cacggtcgca accagcccct tgctcccctg cct
//
LOCUS       DAOSWW010000889         1292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_97094_length_1292_cov_4.299111, whole genome shotgun sequence.
ACCESSION   DAOSWW010000889 DAOSWW010000000
VERSION     DAOSWW010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97094_length_1292_cov_4.299111"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PK442_13965"
     CDS             <1..991
                     /locus_tag="PK442_13965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO88806.1"
                     /translation="GATLTTEDASALEREAYALKGVAPEIFSRTQLVYGNMNWSTLVT
                     GSTSAIFDIREWKIADGRFYIDDEARGGAKIVVLGATVAKNLFGTEDPIGKIVRIRNV
                     PMEVVGVLAAKGQTPWGQDQDDTAFVPFATAQRSLFRRGIANSVQRITVAAVNGDSVS
                     EAEREINAILRQRRRLNEGEASDFDVRNMAEMLDAAAQSTKVMSLLLGAVASVSLLVG
                     GIGIMNIMLVSVTERTREIGIRMAIGARASDIRTQFLMEALLLSIAGGIIGIVLGMSA
                     SRLITGFLGWPTVVSTGSIVLAFGFSVFVGIFFGFYPAWKASLLNPIEALRYE"
     gene            complement(1082..>1292)
                     /locus_tag="PK442_13970"
     CDS             complement(1082..>1292)
                     /locus_tag="PK442_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88807.1"
                     /translation="EEGREEGREEGLEEGLEKGQLEMGISIALAMLEAGEPEEKILRY
                     TGFSSAQLAEIRDGRKSSGKKPSSR"
BASE COUNT          204 a          440 c          411 g          237 t
ORIGIN      
        1 cggcgcgacg ctcaccacgg aggacgcctc cgccctggag cgcgaggcgt acgcattgaa
       61 gggcgtggcc ccggagatct tcagccgcac gcagctcgtc tacggcaaca tgaactggtc
      121 caccctggtg acggggagca cgtccgccat cttcgacatc cgcgagtgga agatcgccga
      181 cgggcgcttc tacatcgacg acgaggcgcg ggggggcgcg aagatcgtcg tgctcggggc
      241 cacggtcgca aagaacctct tcggcacgga ggacccgata gggaagatcg tccgcatccg
      301 caacgtcccg atggaggtcg tcggcgtgct tgccgccaag gggcagacgc cgtgggggca
      361 ggaccaggac gacaccgcgt tcgtcccctt cgccacggcg cagcgcagcc tcttccggcg
      421 gggcatcgcc aactccgtcc agcggatcac cgtcgcggcg gtcaacggcg actccgtctc
      481 cgaggcggag cgcgagatca acgccatcct ccgtcagcgg cggcggctca acgaaggaga
      541 ggcgagcgac ttcgacgtgc ggaacatggc ggagatgctc gacgccgcgg cgcagtccac
      601 gaaggtgatg tcgctgctgc tcggggcggt ggcgtccgtc tcgctgctgg tggggggcat
      661 cggcatcatg aacatcatgc tcgtctccgt caccgagcgc acccgcgaaa tcggcatccg
      721 catggccatc ggcgcgcgcg cctccgacat ccgcacccag ttccttatgg aagcgctcct
      781 cctctccatc gcggggggaa tcatcggcat cgtgctgggg atgtcggcct ccaggctcat
      841 cacgggcttc ctcggctggc cgacggtcgt ctccacaggc tccatcgtgc tcgccttcgg
      901 cttctccgtc ttcgtgggga tcttcttcgg cttctacccc gcgtggaagg cctcgctgct
      961 caacccgatc gaggcgctgc ggtacgagta ggggacgaaa cagagcaaaa gcgcgctctt
     1021 cctctccgat tggaagggcg cgcctttgtt cacattgctg cttcacgccg actaggtcgg
     1081 gttacctgga cgagggtttt ttcccgctgg acttccggcc gtcgcgtatc tctgccaact
     1141 gcgcggagga gaagccggta tagcgcagta tcttctcctc cggctcgccc gcttcgagca
     1201 tcgccagagc tatggagatc cccatctcga gctgcccttt ctcaagccct tcctcaagtc
     1261 cttcttcgcg gccttcttcg cggccttctt cg
//
LOCUS       DAOSWW010000890         1291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_97219_length_1291_cov_3.586570, whole genome shotgun sequence.
ACCESSION   DAOSWW010000890 DAOSWW010000000
VERSION     DAOSWW010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97219_length_1291_cov_3.586570"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1291)
                     /locus_tag="PK442_13975"
     CDS             complement(<1..>1291)
                     /locus_tag="PK442_13975"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOO88808.1"
                     /translation="GQGDRKDRGANHFGHPKLLKRVVGGHWGLAPKLQALALENKIEA
                     YNFPQGVISHLFRDIAAKKPGTITHIGLKTFVDPRLEGGKLNARTTEDLVEIVEIGGT
                     EYLRYMPFPIDVALVRGTFCDAAGNATFEKEALALEVVAMCQAAKNSGGIVVLQVEKV
                     LPKSELNAKLVRLPGIYVDAIVEATPERHMQTFGVQYNPSYCGDAKASIGSIEPLAMD
                     ERKIIARRCAMELGKDVVINLGIGMPEGISIVASEEGVEDQMILTVESGPVGGIPVGG
                     LDFGAAINPDCILDQPVQFDFYDGGGLDIAFLGLAQADRFGNVNVSKFGPKIAGCGGF
                     INITQTAKKVIYCGTFTAGGLEIHAENGALKIIKEGKIKKFIDDVEQITFSGTYAVEK
                     GQPVLYVTERAVFELTEDGLTLTEIAPGVSLENDVLAH"
BASE COUNT          261 a          398 c          309 g          323 t
ORIGIN      
        1 atgcgccaaa acgtcgtttt ccaaagagac gcccggggcg atctcggtca gcgtcagccc
       61 gtcctcggtg agttcgaaga cggcgcgctc cgttacataa aggacgggct gccccttttc
      121 cacagcgtat gttccgctga aggtaatctg ctccacatca tcgatgaatt ttttgatttt
      181 cccctcttta atgatcttca gcgcaccgtt ttcggcatgt atttccaatc ctcccgcggt
      241 gaaggtaccg caatagatca cttttttcgc ggtctgggtg atgttgatga accccccgca
      301 cccggcgatc ttggggccga acttgcttac gttcacatta ccgaagcgat ctgcttgcgc
      361 caagcccaaa aaggctatat caaggccgcc tccatcataa aaatcgaact gaacaggctg
      421 atccagaatg caatcgggat tgatagccgc gccgaagtcc aaaccaccta caggtattcc
      481 gccaacgggg ccggactcca ccgtgaggat catctggtct tctactcctt cttcgctggc
      541 gacgatcgat atcccctcgg gcatacctat accgaggttg atcacaacat ccttcccgag
      601 ctccattgcg caccggcggg cgataatttt ccgttcatcc atagccaaag gctctataga
      661 accgattgag gctttcgcat ctccgcaata gctggggttg tactgaacgc cgaacgtttg
      721 catgtgcctc tccggcgtgg cttctacgat cgcgtcgaca taaattccag ggagacggac
      781 cagcttggcg ttgagttcgc tcttcggaag caccttctcc acctgcagaa cgacgatacc
      841 tccgctgttc ttcgcggctt ggcacatggc cacgacttcg agcgccagcg cctctttttc
      901 gaacgtcgcg tttccggccg cgtcgcagaa cgtaccccgt accagggcta catctattgg
      961 aaacggcatg taccgcaaat attccgtacc gcctatttcg acgatttcca ccaggtcttc
     1021 cgttgttctc gcattcaatt tgccgccctc aagacgaggg tcaacgaatg ttttcaaccc
     1081 gatgtgcgta atcgttccgg gctttttcgc cgcgatgtcc ctgaacagat gcgatatgac
     1141 tccctgcggg aagttgtacg cttcgatctt gttttccaga gccagagcct gcagcttcgg
     1201 agcaagcccc caatgccctc ctacgactcg tttgaggagc ttcggatgtc cgaagtgatt
     1261 cgctcctctg tccttcctgt ctccctgccc g
//
LOCUS       DAOSWW010000891         1290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_97357_length_1290_cov_2.720648, whole genome shotgun sequence.
ACCESSION   DAOSWW010000891 DAOSWW010000000
VERSION     DAOSWW010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97357_length_1290_cov_2.720648"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PK442_13980"
     CDS             <1..741
                     /locus_tag="PK442_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO88809.1"
                     /translation="EGVAAAEHTAAVAKEAVDRVADVIEDVHSVGGKSQESVEKIRAL
                     AASVENISRFVSVISSIADQTNLLALNAAIEAARAGDAGRGFAVVADEVRKLAEESNR
                     AAREVAAIISGLDESARESIVATEEAERIMSRTAVKADDVRQRLAGVLTEIGNISGVI
                     SHIASVAQNQAASSEEMASAIDQVSHSTIDVVQRVDAIRSASGEMAGASEGVAREASE
                     MAERAERMQQLLARFILSRDEQGLAPID"
     gene            complement(822..>1290)
                     /locus_tag="PK442_13985"
     CDS             complement(822..>1290)
                     /locus_tag="PK442_13985"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HOO88810.1"
                     /translation="QLLDPGAAALAALRRKERDIAKMDECIFLLEKLHADRDPQEALL
                     GAYWNTVLHATIFKATGNTLLSRLYESLLEMSEKGISAMRMEVLDSAAPERTEQILEQ
                     HRLLVSAIKEQDVKTAREASKKHLKFTIDTLVELSRVSPVSNFFAERMDSALE"
BASE COUNT          244 a          375 c          431 g          240 t
ORIGIN      
        1 gagggcgttg ccgcggcgga acatacggcg gcggttgcaa aggaggcggt ggaccgggtg
       61 gccgacgtca tcgaagacgt ccactcggtc ggcggcaagt cgcaggagag tgtggagaag
      121 atccgcgccc tcgcggcttc ggtggagaat atctctcgct ttgtctccgt catcagctcc
      181 atagcggatc agacgaatct tctcgcgttg aacgccgcga tcgaagccgc cagggcagga
      241 gatgccgggc gggggttcgc cgtcgtcgcc gacgaagtga ggaagcttgc ggaggagtcg
      301 aacagggctg cgcgcgaagt ggccgcaatc atctccgggc ttgacgagag cgcgcgcgaa
      361 tccatcgtcg cgaccgagga ggcggagcgc atcatgtccc ggacagccgt gaaggcggac
      421 gacgttcggc agcgcctcgc cggcgttctt accgagatcg gcaatatctc cggtgtgatc
      481 tcgcacatcg cctccgtggc gcagaatcaa gccgcgtcaa gcgaggagat ggcgagcgcg
      541 atagatcagg tctcgcactc gaccatcgac gtcgtgcagc gggtggatgc gatacggagc
      601 gcatccggcg agatggcggg cgcatccgaa ggcgtcgccc gcgaggcttc cgaaatggcg
      661 gagcgcgccg agaggatgca gcagctcctc gcacgcttca ttctctcccg ggacgagcag
      721 gggcttgctc cgatcgacta gaacagaaag agagcataca aagaaggagc cctctcggtg
      781 aagggctcct tctttgtgtt tgtgtcactg tttccgtgtc gtcactccag ggcggaatcc
      841 atccgctcgg caaaaaagtt cgaaacagga gagaccctgc tcagttccac cagcgtgtcg
      901 atagtaaact tgagatgctt cttcgaggcc tctctggctg ttttcacatc ctgctccttt
      961 atggcggaga cgagcaggcg gtgctgctca aggatctgtt ccgtccgctc aggcgcggct
     1021 gagtcgagca cctccatccg catcgccgag atcccctttt ccgacatttc gagcaggctc
     1081 tcgtagagcc gtgagagaag ggtgttcccc gtcgccttga agatggtcgc gtggagcacc
     1141 gtgttccagt aggctccaag caaagcctcc tgcggatcgc ggtcggcgtg gagcttttcg
     1201 agaagaaaga tgcactcgtc catcttggcg atgtcgcgct ccttgcgccg gagcgcggcg
     1261 agcgcggctg ctcccggatc gaggagctgg
//
LOCUS       DAOSWW010000892         1289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_97491_length_1289_cov_2.317666, whole genome shotgun sequence.
ACCESSION   DAOSWW010000892 DAOSWW010000000
VERSION     DAOSWW010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97491_length_1289_cov_2.317666"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="PK442_13990"
     CDS             <1..160
                     /locus_tag="PK442_13990"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOO88811.1"
                     /translation="LGGVRVNGRGASTDVIEASIKAYIDGINRLFAAQARIAGQEAQA
                     AEEARKAV"
     gene            240..>1289
                     /locus_tag="PK442_13995"
     CDS             240..>1289
                     /locus_tag="PK442_13995"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOO88812.1"
                     /translation="MGRTLASAIIAAHSTEAAREGEICKVNVDFAFANDITAPPAIVS
                     FKEMGAEKVFDKERCAVLPDHFTPNKDIASAEQTKKGREFALSQGMLYWEVGRVGIEH
                     AFLPEKGYILPGDIVIGADSHTCTYGALGALSTGVGSTDLAAVWALGETWLKVPPTIR
                     VNYEGKRPAWVSGKDMILSLIGRIGVQGARYMALEFGGDGVEALPMDDRFTIANMAIE
                     AGGKAGLFVPDAKMLAYANARAARKFSPVYPDADAAYLRTETFDMEKMVPVAAMPHSP
                     DNVKPVRECGSPEIHQVFIGACTNGRIREIAAAAKMLEGKQVAAGVRCIVIPASYEVY
                     NQALDMGYLRTFTEAG"
BASE COUNT          255 a          410 c          423 g          201 t
ORIGIN      
        1 cctcggcggc gtcagggtaa acggcagggg cgccagcacg gacgtcatcg aagcgagcat
       61 caaggcgtac atcgacggta tcaaccgtct tttcgcggcg caagcgcgca tcgcggggca
      121 ggaggcgcag gcggccgaag aggcgcggaa ggcggtatag ttttccccgc tcacgccgca
      181 acacggcggc ggggttcatc gaatccaaga cagcattgga aaggagtgcg tgtgtacaca
      241 tgggaagaac attggcaagc gccatcatcg ccgcccactc taccgaggcg gcaagggagg
      301 gcgaaatctg caaggtgaac gtcgacttcg ccttcgcgaa cgacatcacg gctcctccgg
      361 ccatcgtctc cttcaaggag atgggagcgg agaaggtttt cgacaaggag cggtgcgcgg
      421 tactccccga ccacttcacg ccgaacaagg acatcgcctc tgcggaacag accaagaaag
      481 ggcgcgagtt cgccctctcg caggggatgc tctactggga ggtcggccgc gtgggcatcg
      541 aacacgcgtt cctccccgag aaggggtaca ttctccccgg cgacatcgtc atcggcgcgg
      601 acagccacac ctgcacctac ggcgcgctgg gcgctctctc cacgggcgtt ggcagcaccg
      661 acctcgccgc cgtctgggcg ctgggcgaga cgtggctgaa ggttcccccc accatccggg
      721 tgaactatga ggggaagcgt ccggcgtggg tgtcgggcaa ggacatgatc ctctcgctca
      781 tcggccgtat cggcgtacag ggcgcacgct acatggcgct cgagttcggc ggcgacggag
      841 tggaagcgct tccaatggac gaccgcttca ccatcgccaa catggcgatc gaggcgggcg
      901 gcaaagccgg actcttcgtg cccgacgcga agatgcttgc gtacgcgaac gcgcgggcgg
      961 cccgaaagtt ctctccggtc taccccgacg cggacgcggc gtacctccgt accgaaacgt
     1021 tcgacatgga gaagatggtt ccagtagctg ccatgccgca ctctcccgat aatgtcaagc
     1081 cggtccgcga atgcggctct ccggagatcc accaggtctt catcggcgca tgcaccaacg
     1141 gccgcatccg cgagatcgcg gccgccgcga agatgctcga aggaaaacag gtggcggccg
     1201 gcgtgcgctg catcgtcatc cccgcctcgt acgaggtcta caaccaggcg ctcgatatgg
     1261 ggtatctgcg taccttcacg gaggcgggc
//
LOCUS       DAOSWW010000893         1287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_97770_length_1287_cov_2.819805, whole genome shotgun sequence.
ACCESSION   DAOSWW010000893 DAOSWW010000000
VERSION     DAOSWW010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97770_length_1287_cov_2.819805"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PK442_14000"
     CDS             complement(<1..522)
                     /locus_tag="PK442_14000"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOO88813.1"
                     /translation="MKKMKKRILVGSLLFVLVVLLVGGVSQYAFASRMMGAGPWGGMS
                     HWHDRGDSAFKAELPRALNPMDFTEEQWKKFDEILGGLRDQGKDFRRGMMKEMWNTRK
                     EIHAMMTAEGFDEEKAKEMIAAVRPSFVSMAQDVLKARNELYGMLNDAQRNKAGAVIE
                     VIGKRLEQPFSDPR"
     gene            752..>1287
                     /locus_tag="PK442_14005"
     CDS             752..>1287
                     /locus_tag="PK442_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO88814.1"
                     /translation="MHRILLLDDDIELCDLLTEYLRPEGFEILVAHDGEEGLAAALSS
                     PVDLVLLDVMLPGKNGFDVLRELRAHSPVPVLMLTAKGDEIDRIVGLELGADDYLPKP
                     FNPRELLARIRAVLRRGERPETALSERLSAGDIEMDLGARTVRVAGKDTELTSVEFRL
                     LEALLRSAGKVVSREKLA"
BASE COUNT          254 a          402 c          353 g          278 t
ORIGIN      
        1 ccgagggtcg ctgaaaggct gctccagccg tttcccgatc acttcgataa cagcgcccgc
       61 cttgttccgc tgcgcgtcgt tcagcatgcc gtacagttcg ttccgggctt tcaagacatc
      121 ttgggccatc gacacgaacg acgggcggac cgcagcgatc atttccttcg ccttctcttc
      181 gtcaaagccc tccgcggtca tcatcgcgtg aatctctttg cgagtgttcc acatctcctt
      241 catcatgccc cgacggaagt cctttccctg atcgcggagc ccgccgagta tctcgtcgaa
      301 cttcttccac tgctcctcgg tgaaatccat cggattcagc gcccggggaa gttccgcttt
      361 gaacgcggaa tctcccctgt catgccagtg ggacatccct ccccaaggcc cggcgcccat
      421 cattctgctt gcgaacgcat actgtgaaac gccgccgaca agcagtacaa ccagtacaaa
      481 tagaagagac cctactaaaa ttcttttttt catctttttc atcgtgtttc gcctcccgtt
      541 gagttttcgg cattcttcgc ccgacgcgaa gagtgtactc ctcgatctct caatgcgctc
      601 ttcgcgcttt gtggataaag gtcaagaaac gtaaagctct tgatactcat cttttcaatt
      661 ccttacaccc ctccaaagcc tgtcgtcgta taatgaacgg atagagagcc gccgaagagg
      721 cggacttcgc acacccgggg aggaaaggaa cgtgcatcga atactgctgc tcgacgacga
      781 catcgaactc tgcgacctgc tgactgaata cctgcgcccg gaggggtttg aaatactcgt
      841 agcacacgac ggcgaggagg ggcttgccgc cgcgctttcg tcgccggtcg acctggtgct
      901 tctcgacgtc atgctgccgg ggaaaaacgg gttcgacgtg ctccgcgagc tgcgcgcaca
      961 ctctcccgtt cccgtgctca tgctcaccgc caagggcgac gagatcgaca gaatcgtcgg
     1021 gctggagctg ggcgccgacg actacctccc gaagccgttc aatccgcgcg agctgctcgc
     1081 tcggattcgc gctgtcctca ggagggggga gcggccggaa acagcgcttt ccgaacgtct
     1141 gagcgcaggg gatatcgaga tggacctcgg cgcgagaacc gtacgcgtcg cagggaaaga
     1201 cacggagctg acgtcagtgg agttcaggct gctggaggcc ttgctgcgct ccgccggaaa
     1261 ggtcgtctcg cgggaaaagc tggcgat
//
LOCUS       DAOSWW010000894         1287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_97782_length_1287_cov_2.629870, whole genome shotgun sequence.
ACCESSION   DAOSWW010000894 DAOSWW010000000
VERSION     DAOSWW010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97782_length_1287_cov_2.629870"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PK442_14010"
     CDS             <1..774
                     /locus_tag="PK442_14010"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HOO88815.1"
                     /translation="VLGKKEKEPEAPAKKSNGGPKVPTIFLKVFKNVPASTLQTLFPD
                     VTIQMTLIDKGKIALPIIAGFLSVVNRVIPALLVVGTLIAAIVAGRNIDWAHFKEQLF
                     PILAAFSIVGTIAFKVFTKYKLTKEKHQAKLMKTLYFHNLDNNAGVFDFLVHEAEEEE
                     CKEALLAYYFLLTGKDSEGKAYSQDELDDRIEQWMKEKFGVTLDFEVDDALRKLEDKK
                     LLVKEGDRYLVPSIDDTLVKLDELWDTIFTYSNEAEGNA"
     gene            complement(955..>1287)
                     /locus_tag="PK442_14015"
     CDS             complement(955..>1287)
                     /locus_tag="PK442_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOO88816.1"
                     /translation="LLIRGGGVSPYELTVFFCVFVMLQIWNLFNARCLGLKTSAFSGL
                     TENRWFAAIVLLVLAGQILMVQFGGKVFRTVPLSLVDWLLIIVGTSPVLLVGELLRFR
                     ARRRETAA"
BASE COUNT          292 a          399 c          389 g          207 t
ORIGIN      
        1 gtgctcggaa agaaagagaa ggagccggaa gcccccgcga agaagagcaa cggcgggccg
       61 aaggtgccca cgatcttcct gaaggtcttc aagaacgttc cggcctcgac gctgcagacg
      121 ctcttccccg acgtcaccat ccagatgacc ctgatcgaca agggaaagat cgccctgccg
      181 atcatcgcgg gttttttgag cgtggtgaac cgcgtcatcc ccgcgctgct cgtcgtcggc
      241 acgctgatcg ccgcgatcgt cgccggacgg aacatcgact gggcgcactt caaggagcag
      301 ctcttcccga tccttgcggc cttctccatc gtcgggacga tcgccttcaa ggtcttcacg
      361 aagtacaagc tcacgaagga gaagcaccag gcgaagctga tgaagacgct ctacttccac
      421 aacctggaca acaacgccgg agtcttcgac tttctcgttc acgaggcgga ggaggaagag
      481 tgcaaggaag cgctgctcgc ctactacttc ctgctgacag ggaaggattc cgagggaaag
      541 gcgtactccc aggacgagct ggacgaccgc atcgagcagt ggatgaaaga aaagttcggc
      601 gtgacgctgg attttgaggt ggacgacgcg cttcgcaagc tggaggacaa gaagttgctg
      661 gtgaaggaag gcgaccgtta cctcgtcccg tcgatcgacg acacgctggt caagctcgac
      721 gagctgtggg acaccatctt tacgtacagc aacgaggcgg agggcaacgc gtaacgaaga
      781 agcatagacc ggcccgcaca gtaagtacag ccggagagcg gtccgccgcc ctccggctgt
      841 tttttcgctc atgcgtgcgc acgatgctga gttttcgtcg cgtgaagccc tgcggttccc
      901 ccgcagaact cccgaccgcc gcgtgcaggc aaccctctcc cccgccgggg aaactcaggc
      961 cgccgtttcc cgccgccgcg ccctgaagcg aagcagctcg ccgacgagca gcaccggcga
     1021 cgtgccgacg atgatcaaca gccagtcaac aaggcttaag ggcacagttc tgaagacctt
     1081 cccgccgaac tgcaccatca gaatctgccc cgcgaggacg agcaggacga tggccgcgaa
     1141 ccaccggttc tccgtcaggc cgctgaacgc cgaggttttc aggccgaggc agcgcgcgtt
     1201 gaagagattc catatctgga gcatcacaaa gacgcagaag aagacggtca gctcgtaggg
     1261 agagacgccg ccgccccgta tcagcag
//
LOCUS       DAOSWW010000895         1283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_98239_length_1283_cov_3.783388, whole genome shotgun sequence.
ACCESSION   DAOSWW010000895 DAOSWW010000000
VERSION     DAOSWW010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98239_length_1283_cov_3.783388"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..>1283)
                     /locus_tag="PK442_14020"
     CDS             complement(179..>1283)
                     /locus_tag="PK442_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO88817.1"
                     /translation="TVRSAVAVQRPSAGLRGATSYNTDDAPNRRQGVYSLSAAISHRF
                     DVSGVYSAQEKQLLLQYNALAADHLALINNTLATAESLYWRSFIARENSALQKEILAQ
                     RREDLRITEEKFRQQLIPRLDVVRAQARVEEAESIVVQAESAYQDTLTQLATLAGGIA
                     VTPRSETLLVPSLSVRAGVESALGTRNDVRSAELSLERARVLKTLAAKGMSPSVEGAI
                     GYTILTDSPTQQPDQNEILFSLNVSIPVYDGGKTKEDTEEKARSIEAAERSLEARRLA
                     VREDVLKARTQWEKAVALEASKRKQVARSDEELKITQLMYMEGMGAQIDLLNAQVDNQ
                     RVRTEHLDAIKEMYLALVSLRQAMSEYEPAPAE"
BASE COUNT          270 a          369 c          338 g          306 t
ORIGIN      
        1 cagagcgtcc gaagcgttcg atatgagttc gcggagaaag atgtcacgat tcgaatagac
       61 cgagttgatc ataagattca aaagctgttt ggtctccgcc tgaaagaaaa aagtttcctg
      121 ctgcatagta atcctcctta cagttgcgta tgtttcacgg cttccttgta aaaagacatt
      181 attccgcagg agccggttcg tactcgctca tcgcttgccg caaactgaca agcgcaaggt
      241 acatctcctt gattgcatcg agatgttccg tccgtacacg ctgattatcc acctgggcgt
      301 tgagaagatc gatctgagct cccatacctt ccatgtacat aagctgcgta atcttcagct
      361 cttcatccga gcgggctacc tgcttacgtt tgctcgcttc cagcgcaacg gccttctccc
      421 actgcgtccg cgccttgaga acatcttctc ttacagcaag gcgccgtgct tcaaggcttc
      481 tttccgcagc ttcgatgctc cgggcctttt cctccgtatc ctctttggtc tttccaccgt
      541 catacacagg tatagacaca ttcagagaaa agaggatctc gttctgatcg ggctgctgag
      601 tgggagagtc ggtaagaatc gtgtagccga tagccccttc gaccgagggg gacatacctt
      661 ttgcagcaag cgttttcagc acgcgtgcgc gttccaggct cagttcggct gagcggacgt
      721 cgttccgtgt tccaagcgcg ctctcaacac cggcgcggac cgacaatgag ggaacaagca
      781 aagtctccga cctgggggtg acagcaatgc ctcctgcgag cgtggccaac tgcgtcagcg
      841 tatcctgata cgccgattct gcctgcacga cgatgctctc cgcttcttca accctcgcct
      901 gagccctcac aacgtcaagt ctcggaatga gctgctgacg aaacttttct tcggtaatac
      961 gaaggtcttc gcgcctctgg gcaaggatct ccttttgaag cgcgctgttt tctctggcga
     1021 tgaaagaacg ccagtaaagg ctctctgcag tcgcaagcgt gttattgatc agcgcgagat
     1081 gatcggcggc gagggcattg tactgaagga gcagctgctt ctcctgcgca gagtacacgc
     1141 cgctcacgtc gaaacggtga gatatcgccg cggagagaga gtagactccc tggcggcggt
     1201 tcggcgcatc gtccgtattg tacgacgtcg ctcccctgag accggcgctc ggacgctgga
     1261 ccgccactgc tgaacgaacc gtg
//
LOCUS       DAOSWW010000896         1281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_98476_length_1281_cov_4.065253, whole genome shotgun sequence.
ACCESSION   DAOSWW010000896 DAOSWW010000000
VERSION     DAOSWW010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98476_length_1281_cov_4.065253"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..304
                     /gene="fliE"
                     /locus_tag="PK442_14025"
     CDS             8..304
                     /gene="fliE"
                     /locus_tag="PK442_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOO88818.1"
                     /translation="MKMAGIQSLPAVAAVSEKKDTPRGTGLSFEDLLESSMKQVNDLQ
                     QDSDKMVNRLATGDVDDISEVVLSAQRAEFALRLITEVRNKLVDAYQQLGRMSV"
     gene            315..>1281
                     /gene="fliF"
                     /locus_tag="PK442_14030"
     CDS             315..>1281
                     /gene="fliF"
                     /locus_tag="PK442_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOO88819.1"
                     /translation="MNWFRQALSRGGLFWSSLRPWQRFSIVGASALVLLALILGVYWA
                     GRPTYEPLYSALEVEDQSAIVAWLRENKVDYTLDPAANAILVPRDLVYETRLSLAQGG
                     LPKGGSVGYEIFDTTKMGLSEFQQKITYIRALEGELERTIRQIDAVDYAKVNIVIPEQ
                     RLFLEEQQPSTASVLVRLRSGMQIGPDHVKSIVHLVSHAVQGLVPENITVVDTAGKVL
                     SDLLDQEFFIYSGAGDGRTVSSVQRELERQQERELESKVRVMLERVFGPGKVVVRVKV
                     DLDFDRRTSATKEYIPGETGRGVIRSQQQMEETYTGTGGPIGGAPG"
BASE COUNT          272 a          358 c          385 g          266 t
ORIGIN      
        1 cgatttaatg aaaatggcgg ggatccaatc tctgccggct gtagctgccg tttccgaaaa
       61 gaaggacacg cctcggggaa ccggactttc gttcgaagac ctcctggaga gttcaatgaa
      121 gcaagtaaac gatcttcagc aggattccga caagatggtc aacaggctgg ccacgggtga
      181 tgtcgatgat atctctgaag tagtgctttc ggcgcagcgt gcggagtttg ccctgcgcct
      241 gatcaccgag gtacggaaca aactcgtcga cgcctaccag cagctcggac gcatgtcggt
      301 gtaggaagta gccgatgaac tggtttcggc aagcgctctc gcggggcggc cttttttggt
      361 cgtccctaag accgtggcag agattctcca ttgtcggagc gtcggcgctt gtcctgcttg
      421 ccttgatttt gggagtttac tgggcgggac gtcccacgta cgaaccgctc tattccgcgc
      481 ttgaggttga agaccagtcg gccatcgtcg cctggctccg tgaaaataaa gtggactaca
      541 cgctcgatcc tgccgcaaac gccatcctcg ttcctcgcga tctggtctac gaaacgcgct
      601 tgagtcttgc tcaaggcggg cttcccaagg gaggaagcgt cggatacgag atcttcgaca
      661 cgacgaagat ggggttgagc gagttccagc agaagataac ctatatccgc gctctcgaag
      721 gggagttgga acgtaccatt cgtcagatcg acgcggtaga ctacgcaaag gtgaacatcg
      781 tcattcctga acagcgtctc tttctcgaag agcagcagcc ctcgacggcc tccgtcctcg
      841 ttcgcctccg ttccggaatg cagatcggtc cggatcatgt aaaatcgatt gttcacctcg
      901 tttcccatgc ggtgcaggga cttgttcccg aaaatatcac ggtggtggac actgccggca
      961 aggtgctctc ggaccttctc gaccaggagt tcttcatcta ctccggcgca ggcgacggga
     1021 gaacggtctc gtccgttcag cgcgaactgg agcgtcagca ggagcgtgaa ctggagagca
     1081 aggtccgcgt gatgcttgaa cgggtcttcg gccccggaaa ggtcgttgtc cgcgtgaaag
     1141 tcgatctcga cttcgaccgc cgcaccagcg ccacgaagga gtacatcccc ggggagactg
     1201 gcagaggggt catccgcagc cagcagcaga tggaagagac gtacaccgga acaggcggcc
     1261 ccatcggcgg cgctcccggt a
//
LOCUS       DAOSWW010000897         1281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_98519_length_1281_cov_2.674551, whole genome shotgun sequence.
ACCESSION   DAOSWW010000897 DAOSWW010000000
VERSION     DAOSWW010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98519_length_1281_cov_2.674551"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..>1281)
                     /locus_tag="PK442_14035"
     CDS             complement(227..>1281)
                     /locus_tag="PK442_14035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOO88820.1"
                     /translation="GAMTELRTEREDLSRIVTALPVGVVLLDEERRVRYLNDVGKRLL
                     CVKEEAPHGVPVERILPSGDMYPLVEAAMNGEEDCLTLDLPELDKRYLRICSRRTSSG
                     VLLVISDLTEEYRLEQSRRDFIADAGHELQTPLASIRLAAEFLMEGPKRSEEDEKCLT
                     TIIAQQERMTRLVDDLLLLSRMESEPPLHPEEDVDLCRLLSAVVEENRRHPLAGRIGV
                     EEEYECAAPALLRSEDISRALNNIVENAVKYVHEKFGDEEGGRIQVSLRPEHLFWSIR
                     VEDNGPGISSETAGVLFQRFRRGDSHRARGKWGKGGYGLGLAIARKIVLDAGGDILLH
                     PSDAGAVFEVLLPRKD"
BASE COUNT          233 a          485 c          342 g          221 t
ORIGIN      
        1 cgacgtcgtg gaggccatcc cctcctaccg cccctaccgc ccggcgctcg ggatagacgc
       61 cgcgctccag gagattgaaa acggcatcgg aacgaaatac gatcccgacg ccgtccgcgc
      121 atgcactgcc ctcttcaggg aaaaccgatt cgaattcgac gaatgaaaaa gggcggaaga
      181 gcccgtttcg gagctcttcc gccctgcgtt cctctttcgc cgcggatcag tccttccggg
      241 gcagcagcac ctcgaagacg gcgcctgcat cggacgggtg aagcagtata tcccctcccg
      301 cgtcgagcac gatcttccgc gcgatcgcca ggccgagccc gtagccgccc ttcccccact
      361 tcccgcgcgc ccggtggctg tcgcccctgc ggaaacgctg gaaaagcacg cccgcagtct
      421 cggacgaaat ccccggcccg ttatcctcca cccgaatgct ccagaaaaga tgctccggcc
      481 gaagcgacac ctgaattctg ccgccctctt cgtcgccgaa cttctcatgg acatacttca
      541 ccgcgttctc cacgatattg ttcaacgccc gggaaatgtc ttcgctccgc agcagcgcag
      601 gcgccgcgca ctcatactct tcctcgaccc cgatacgccc cgcgagagga tgcctccggt
      661 tctcctcgac cacggcggac agcagcctgc acaggtcgac atcctcctcc gggtgcagcg
      721 gcggttccga ctccatccgc gaaagcagca gcagatcgtc gacgagccgg gtcatccgct
      781 cctgctgcgc gatgatggtc gtcagacact tttcgtcctc ttcgctccgc ttcggcccct
      841 ccatcaaaaa ctccgcggcc agcctgatgc tcgccagcgg cgtctgaagc tcatgccccg
      901 cgtcggcgat gaagtcgcgc ctcgactgct ccagccggta ctcctccgtg aggtcggaga
      961 tcacgagcag caccccgctc gacgtccgtc gcgagcagat acgaagatag cgcttgtcca
     1021 gctccggaag atccagcgtc aggcagtcct cttctccgtt catggccgcc tccacaaggg
     1081 ggtacatatc cccggaagga agaatccgct ccacggggac gccgtgcggc gcctcctcct
     1141 tcacgcagag aagccgcttc cccacgtcgt tcaggtaccg gaccctccgc tcttcgtcaa
     1201 gcagcaccac gcccaccggc agcgccgtca cgatgcgcga aagatcctcg cgctccgtgc
     1261 gaagctcggt catcgccccg c
//
LOCUS       DAOSWW010000898         1281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_98543_length_1281_cov_2.230832, whole genome shotgun sequence.
ACCESSION   DAOSWW010000898 DAOSWW010000000
VERSION     DAOSWW010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98543_length_1281_cov_2.230832"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK442_14040"
     CDS             complement(<1..618)
                     /locus_tag="PK442_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO88821.1"
                     /translation="MRHLKPFVLSLLVPALFLCLWAYMAKRAANDVVLPPIGQVAAHF
                     ASPGTSLIGMGSLVSNILISLLRVLAGYLIAVLLAIPAGLLLGHNRLLSELFTPFLGL
                     FRPIPPLAWVPLVLAWFGIASFATLFGVEEGEWYVFLNNLKFSMVFIIFIGAFYPILS
                     GTMHGVSGVRSILVDSARVLGASDRDIFLKVLLPASLPSIVIGLRI"
     gene            complement(718..>1281)
                     /locus_tag="PK442_14045"
     CDS             complement(718..>1281)
                     /locus_tag="PK442_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88822.1"
                     /translation="AFGEAGLKLTHDGADLSANVLLVDLKSTGNLAPALTSGQVDAWV
                     GPDPFPAVAEVRKTGRTVFDLKELPPAGRWSSFPCCVAAARTDFLAERPEVAEGVVKL
                     LTVASEWINENQAEAGRIAAQWIGIPEEATEHATIVYGTDPNEAWLKGAGVYLDVLNG
                     MDSFKGSLKGKTLEEAIPLIFDFSFNK"
BASE COUNT          252 a          398 c          433 g          198 t
ORIGIN      
        1 gatgcgaaga ccgatcacga tggaggggag ggaggcgggc agcagcacct tgaggaagat
       61 gtcgcggtcg gaggccccca gcacccgcgc cgagtcgacg aggatggagc gcacgccgga
      121 gacgccgtgc atcgtccccg agaggatcgg gtagaacgca ccgatgaaga tgatgaagac
      181 catcgagaac ttcaggttgt tcaggaagac gtaccactcc ccctcctcca cgccgaagag
      241 cgtggcgaag ctcgcgatgc cgaaccacgc gagcacgagc ggaacccagg cgaggggcgg
      301 tatcgggcgg aagagtccga ggaagggggt gaagagttcg gagagaaggc gattgtgccc
      361 cagcagcagc cccgcgggga tcgcgaggag aacggcgatc aagtagcctg cgagcacccg
      421 gagcaggctg atgaggatgt tggagacgag agagcccatt ccgatcaggc tcgtccccgg
      481 agacgcgaag tgcgccgcca cctgccctat cggggggagg acgacatcgt tggccgcgcg
      541 cttcgccatg tacgcccaca ggcacaggaa aagcgccgga acaagaagcg agagaacaaa
      601 aggtttcaga tgccgcatat cggtcgcata tcctttctgc ctccgcgcgc gtcgggggcc
      661 cctccgtcga tatacgaggg acccccgtgc gagcggaggc tgtgaatctt ctttcgccta
      721 cttgttgaag gagaagtcga agatcagcgg aattgcctct tcgagcgtct tgcccttcag
      781 agagcccttg aagctgtcca tcccgttcaa cacgtcgaga tagactccgg cgcccttgag
      841 ccacgcttcg ttggggtcgg tgccgtagac gatggtggcg tgctcggtcg cctcctccgg
      901 gatgccgatc cactgcgcgg ctatccgccc cgcctccgcc tgattctcgt tgatccattc
      961 ggaggcgacg gtaagcagct tcacgacacc ctcggccacc tcgggacgtt ccgcgagaaa
     1021 gtcggtccgg gccgccgcga cgcagcaggg gaagctggac caccgtcccg ccgggggaag
     1081 ctccttcagg tcgaagacgg ttctacctgt ctttcggact tcggccaccg cggggaacgg
     1141 atcgggaccg acccatgcgt cgacctgccc gctggtcagc gcgggcgcga ggttccccgt
     1201 ggacttcagg tcgacgagga ggacgttcgc cgacaggtcg gcgccgtcgt gcgtcagctt
     1261 cagccccgcc tcgccgaacg c
//
LOCUS       DAOSWW010000899         1280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_98604_length_1280_cov_4.285714, whole genome shotgun sequence.
ACCESSION   DAOSWW010000899 DAOSWW010000000
VERSION     DAOSWW010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98604_length_1280_cov_4.285714"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PK442_14050"
     CDS             <1..277
                     /locus_tag="PK442_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOO88823.1"
                     /translation="RCHHEKWDGSGYPDGLAGESIPLCGRIVALADVYDAISSKRVYK
                     PSFPREQCWDIINRGRGSHFDPRLVDIFNRNEDYFWNIVEELRDIDD"
     gene            311..>1280
                     /locus_tag="PK442_14055"
     CDS             311..>1280
                     /locus_tag="PK442_14055"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOO88824.1"
                     /translation="MYGQFSRFVGRISLRRIIRIAFVFQILIPVSFLGWMFFRTSRES
                     VEALSMRLGSEVAARIAEHLQSYMAVPLLVNSLNADALESGRVDIASPRIWQPFFTER
                     IRAFPSIAYTFIGTPRGEFFGARRFKENVELFVASPETTGGASVYYSVDPRGMTGDEI
                     ISYPGFDPRTRPWYTAGEHADTPVWSEIYRHFALDDLAITAVQALRGEDGSLVGVLGV
                     DLPLSAINSFLRELKVGAEGQIFILDNDGNIVADSTVDKPFHIRNGAFSRIAGVASPN
                     PAIRAAAERMAREEQGERTGRMFSVENSAGISEYVRTRPFRSFGLDW"
BASE COUNT          244 a          375 c          376 g          285 t
ORIGIN      
        1 ccgctgtcat cacgaaaagt gggacggcag cggatacccc gacgggctcg cgggagagag
       61 cattccgctc tgcggaagga ttgtcgctct cgccgacgtg tacgacgcca tcagcagcaa
      121 gcgtgtctac aaaccatcct tcccgcggga gcagtgctgg gatattatca acaggggcag
      181 gggaagccat ttcgatccgc ggcttgtcga catattcaac cgcaacgagg attacttctg
      241 gaatatcgtc gaagagctac gggatatcga cgattgagtt cgccgtaggt gacgggaggg
      301 ggatcttttt atgtacggac agttctcccg ttttgtcggg cgtatttcgc tccggaggat
      361 cattcgcatc gcttttgtct ttcagatact cattcctgtc tctttcttgg ggtggatgtt
      421 cttcagaacg tcccgggaat cggtcgaagc gctctctatg cgcctgggga gcgaagtggc
      481 ggcgcggatc gcggagcatc tccagtcgta tatggctgtc cctcttctcg tgaactctct
      541 caatgcggac gcgctggagt cggggagggt cgacatcgcc tctccccgga tctggcagcc
      601 ctttttcacc gaacgcatca gggcctttcc gtcaatagcg tataccttca tcggcacgcc
      661 ccgtggagag ttcttcgggg cgcgccgctt caaggagaat gtcgaactct tcgtagcaag
      721 tcccgagaca acgggaggcg cttccgtcta ttactccgtc gatccccggg ggatgacggg
      781 agacgaaatc atctcctacc ccggtttcga cccccgcacc cgtccgtggt atacagcggg
      841 cgaacacgcc gacactcccg tctggagcga aatctaccgc cattttgcac tcgatgacct
      901 ggcgattacc gccgtccagg ctcttcgcgg ggaggacggc tctctggtcg gagtgctcgg
      961 cgtcgatctc cccctctccg ctatcaacag ctttctcagg gagctgaagg tgggcgccga
     1021 aggacagatt ttcatcctcg acaatgacgg aaacattgtg gcggattcga ctgtcgataa
     1081 gccgtttcac atccgcaacg gcgccttttc ccggattgcg ggcgtggctt ctcccaatcc
     1141 ggcaataagg gcggccgctg aaaggatggc gcgcgaagag cagggagagc gcacaggccg
     1201 aatgttctcc gtcgaaaact ccgcaggtat ttccgagtac gtccggacac gcccgttccg
     1261 atctttcggt ctcgactgga
//
LOCUS       DAOSWW010000900         1280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_98626_length_1280_cov_3.093061, whole genome shotgun sequence.
ACCESSION   DAOSWW010000900 DAOSWW010000000
VERSION     DAOSWW010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98626_length_1280_cov_3.093061"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK442_14060"
     CDS             <1..263
                     /locus_tag="PK442_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HOO88825.1"
                     /translation="GWRYLPNLGKGGATLSHATLYPQEMVVRQVWTGEGSLRWTPLPY
                     EQHPLQCRIIGELAGLPVAQYLGAKMVKGTARLNVGDSRMLP"
     gene            281..1087
                     /locus_tag="PK442_14065"
     CDS             281..1087
                     /locus_tag="PK442_14065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO88826.1"
                     /translation="MNGYYENKVSAVTGAASGIGLGLVEHLLAKGAAGVFMGDVKEEN
                     LTRESKRLNEKYPGKVFPVLTDVTKQEQVAEFVSKAKAFDGHLDFVFNNAGVGLTLPT
                     EKVTFEMWKFVVDLNLMGVVYGTYSAIPVMREQGFGHIVNTGSITGRVPVPYQAVYAA
                     TKAAVISMTESLQFELETEGLNFSVFCPGNVATAIFGDVPPPPDAIGVDDAVEYILAE
                     VEKKSLVIILPQQMRDFDAVYREHREEYDAFARKMAAERRENYRTKGTYF"
BASE COUNT          255 a          367 c          426 g          232 t
ORIGIN      
        1 tcggctggcg atatctgccg aacctcggta agggaggcgc gacgctgagc catgcgacgc
       61 tctatccgca ggagatggtc gtccgccagg tatggacggg cgagggatcg ctccgctgga
      121 cgccgctccc ctacgagcag catccgctcc agtgcaggat tatcggagaa ctggccggac
      181 tgccggtggc gcagtatttg ggcgcgaaga tggtgaaggg gaccgccagg ctcaacgtgg
      241 gcgattcgag aatgctgccg tgaagaaggg agagactgtg atgaacgggt attatgagaa
      301 caaagtctcc gcggtaacgg gggccgcttc gggaatcggt cttgggctcg tcgagcatct
      361 gctggcaaag ggcgccgccg gcgtcttcat gggcgacgta aaggaagaga acctgacgcg
      421 ggagtcgaag cgcctgaacg agaagtaccc gggaaaggtc ttccccgtgc tcaccgacgt
      481 gacgaagcag gaacaggtcg ccgagttcgt ctccaaggcg aaagcgttcg acggacatct
      541 cgactttgtc ttcaacaacg ccggagtggg cttgacgctt cccacggaga aggtcacctt
      601 cgagatgtgg aagttcgtcg tcgacctcaa cctgatgggc gtcgtctacg gcacgtacag
      661 cgcgatcccc gtcatgcgcg agcagggctt cggacatatc gtcaataccg gctcgatcac
      721 ggggcgggtt cccgttccgt accaggccgt ctacgctgca acgaaagccg ccgtgatctc
      781 catgacggag agcttgcagt tcgagctgga gacggagggg ctcaacttca gcgtcttctg
      841 ccccggcaac gtcgccaccg cgatcttcgg cgacgttccg ccgcctcccg acgctatcgg
      901 cgtggacgac gcggtggagt acatcctcgc ggaggtggag aagaaatcgc tcgtgatcat
      961 cctcccgcag cagatgcgcg attttgacgc ggtctataga gaacacaggg aggagtacga
     1021 cgccttcgcc cggaagatgg ccgccgagcg ccgggagaac tacaggacaa agggaacgta
     1081 cttttaggcg cgcgcgccga cgaaggaaag gaggcgcgat ccgatgcgcc gcggactgtt
     1141 tgacgaagcg tgtcttccgc tggagaccgt gttgctgttc atcgccggga tgacggtgtt
     1201 catcgccgga atcctgctct tccccgtgta cgccgggctg ctggcctact atgagaacgg
     1261 cctgtacggc cttctgctct
//
LOCUS       DAOSWW010000901         1279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_98818_length_1279_cov_2.189542, whole genome shotgun sequence.
ACCESSION   DAOSWW010000901 DAOSWW010000000
VERSION     DAOSWW010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98818_length_1279_cov_2.189542"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PK442_14070"
     CDS             complement(<1..748)
                     /locus_tag="PK442_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOO88827.1"
                     /translation="MRKRAGVGILDRFILKELSGSFLFGVTSFTMVFVAGDLLFQAAN
                     LIIEQGVSLGVVARLFIYRLPEVVMLTLPMASLLSSLLTFSRLSTNSELVALKSAGIP
                     FHRILRPVFLASILVSLASTIGAETVVPFANRAAENLMKFEILQEKPSLLKEKVFLRE
                     EKDGVLQRVLYLGELKPKDGLMKDIIIQEFEEGRLRRISLADEGLWAGGEWWIENGQV
                     FEVTPAGKVQTLFRFERQRLILNLSPVQIEQ"
     gene            complement(748..1257)
                     /locus_tag="PK442_14075"
     CDS             complement(748..1257)
                     /locus_tag="PK442_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOO88828.1"
                     /translation="MKTIKLLVLDVDGTLTDGGVYMDGRGGEFKRFDVRDGMGIVRLR
                     AAGVRTAFLSGRRSEATSQRAADLGVELLFNGVREKLPVLRSLLSDLSLSAEEAAYMG
                     DDVNDVDCLRAAGLAFAPADAVPEAKSAADFVTSAKGGGGAVREAAEHIISLNARCEG
                     RSSGAEERP"
BASE COUNT          283 a          413 c          349 g          234 t
ORIGIN      
        1 cctgctctat ctgcacggga cttaaattaa ggatgaggcg ctgccgttca aagcggaaga
       61 gcgtctgcac tttgccggcg ggcgttacct cgaacacctg gccgttctct atccaccatt
      121 cgccccctgc ccataatcct tcgtcggcca gggagatcct tcgcaaacgc ccctcctcga
      181 actcctggat aatgatgtct ttcatgagac cgtctttcgg cttgagctct ccgagataga
      241 gcacccgctg gagcacaccg tccttttctt cccgaagaaa gactttttct ttcagcagcg
      301 acggtttctc ctgcaggatc tcgaatttca tcaggttctc cgccgccctg ttggcaaacg
      361 gaacgacagt ctccgccccg atcgtcgagg caagactcac gaggatggag gcaaggaaga
      421 ctggacgaag aatcctgtga aacggtatcc ccgccgactt caaggcgacc agttcgctgt
      481 tggtggaaag cctgctgaac gtcagcagcg acgagagcag agaggccatg gggagcgtga
      541 gcatcaccac ctcgggaagc cggtagatga agaggcgggc aactacgccg agcgagacgc
      601 cctgctcgat gatcaggttc gccgcctgga agagcagatc tccggcgaca aagaccatcg
      661 tgaaagaggt cacgccgaaa aggaaggagc cggaaagctc cttcaggata aaacgatcaa
      721 gaataccgac cccggccctt tttctcatca cggtctctct tcagctccgg aagaacgccc
      781 ctcgcagcgc gcattcagcg aaatgatatg ctccgccgcc tcgcggacag ccccgcctcc
      841 gcccttggcg gacgtcacga aatccgccgc ggacttcgcc tccgggactg catccgcagg
      901 agcaaaagca agaccggcag cccggagaca gtcgacgtcg ttcacgtcgt cccccatgta
      961 ggccgcctcc tctgcggaaa gtgaaagatc cgacagtagc gaacgcagca cggggagctt
     1021 ctccctcacg ccattaaaaa ggagttccac gccgagatcg gcggcgcgtt gggacgtcgc
     1081 ttccgagcgg cgtccgctga ggaacgccgt tcggactccc gccgctcgca gacggacaat
     1141 ccccataccg tcgcggacgt cgaaacgctt gaattccccg ccgcggccgt ccatatacac
     1201 tccgccgtcg gtaagcgtac cgtcgacatc gaggacaagc agcttaatcg tcttcatgat
     1261 ccttcccctt gaagacgcg
//
LOCUS       DAOSWW010000902         1279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_98848_length_1279_cov_1.807190, whole genome shotgun sequence.
ACCESSION   DAOSWW010000902 DAOSWW010000000
VERSION     DAOSWW010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98848_length_1279_cov_1.807190"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..260)
                     /locus_tag="PK442_14080"
     CDS             complement(117..260)
                     /locus_tag="PK442_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88829.1"
                     /translation="MENTMDSGSEAMIGTLSSPRSGERTMPDADSRDEPVGAAGSDEV
                     IKG"
     gene            475..1077
                     /locus_tag="PK442_14085"
     CDS             475..1077
                     /locus_tag="PK442_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88830.1"
                     /translation="MLSLEFPYTTCNFVRPEALCLAAIEAVDTGSVVRNVQSCGTDVV
                     FWQDKYNGLFFPYIGMTLVKDDERKNLLPEMQEKNASTFLSVFKTPFVESAFRSLAEK
                     LDMEVLERVEIKVMSADEKFVTQSTVLDALTLYSASGVFSAEALARMMQDLKSEDANL
                     HTKEQYWGNLFGYTLAPFAASKFNFVVKRHSVKLRYASFD"
BASE COUNT          301 a          344 c          351 g          283 t
ORIGIN      
        1 gaacggaact tcgtttttta gcgctgtcga tgaacaaacg ggataacgtc acccgtaaaa
       61 aagctcaaga atttcgcgag aaaatttgac agaccccccc gtttttgaat ataatctcac
      121 cccttgatca cttcatccga gcccgccgca ccgacgggct cgtcccttga atctgcgtcc
      181 ggcatcgtcc gctctccgct cctcggagac gaaagagttc cgatcatcgc ttcagatccc
      241 gaatccatag tattctccat cgtttcacgt ccggagtacg acgtaacatg tcgcttccat
      301 gctgtacgat tctcgtccgg tacgccgctt cctgtcggat gcggggcaca ttgacaatcg
      361 aaaagagaag gcgccgacaa aagcgtttcg ggaacttcgc cgtgcgccgc ggcggagcat
      421 gcggagaaga gaagagttcg ttgcgaggat gaatgaaggg aggatgttcg aaatatgctg
      481 tccctggagt ttccgtacac cacgtgcaat tttgttcggc cggaggcgtt gtgcctggcc
      541 gcgatcgagg ccgtcgatac gggatcggtc gtaaggaacg ttcaaagctg cggaaccgac
      601 gtcgtcttct ggcaggacaa atacaacggc cttttctttc cctatatcgg catgacgctt
      661 gtaaaggacg acgagcgaaa gaatctcttg cccgaaatgc aggagaagaa cgcttccaca
      721 tttctgagcg tcttcaagac tccgttcgtc gagtcggcgt tccgttctct cgcggagaaa
      781 ctggatatgg aagtccttga aagggtagag atcaaagtca tgtcggcgga cgaaaagttc
      841 gtcacccagt cgaccgttct ggacgccctg acgttgtaca gcgcatccgg tgtcttttcc
      901 gcggaagcgc ttgcacgaat gatgcaggat ctgaaatcgg aggacgcaaa cctccatacg
      961 aaagagcagt actgggggaa ccttttcgga tatacgctgg ctcccttcgc cgcctcgaag
     1021 ttcaacttcg tcgtcaaaag acactccgtc aagcttcgat acgcgagctt cgattagccg
     1081 tccttcgtcg gctgcctctc cggcgacttc gacggcgtat gctcttcgat ggaaagctga
     1141 agcaaacggt cacggaacga atcgttccga acacgcggag cttccggggg aagtccggct
     1201 gagagtcgct ccgaagagcg gcggaagcgg agcggagaaa attttcactg aaagaagaca
     1261 tagccagtgg attcgcttt
//
LOCUS       DAOSWW010000903         1278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_98974_length_1278_cov_1.774325, whole genome shotgun sequence.
ACCESSION   DAOSWW010000903 DAOSWW010000000
VERSION     DAOSWW010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98974_length_1278_cov_1.774325"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK442_14090"
     CDS             complement(<1..662)
                     /locus_tag="PK442_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOO88831.1"
                     /translation="MFKESKESEKIVDSVASALTILDCFETAPSLSLGDIHERTGINK
                     TRIMRLAGTLRHMGYVTQDQATGRYALGPKVHSLGGGLSKSYLNIKVLLNSFMKEIVE
                     ETGETASLFVVHGFRRLCLVREESRNAVRYVINEGEERPLYKGAPSKALLAFLPEEQR
                     EAFLEHFSTMRKEYDADAFRRQIVETKERGYASSMAEVSPNSYSIAVPVFCGFGKFYA
                     ALA"
     gene            complement(761..>1278)
                     /locus_tag="PK442_14095"
     CDS             complement(761..>1278)
                     /locus_tag="PK442_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO88832.1"
                     /translation="AAAILLGALIMKGIQPGPMLMRNNPETIFTFFVGFAMVNILVFL
                     IGHFFVQIAYSILRIPVQILAPLILVICFLGAFASENSMFNVYVMLAVGIVAYVLGKM
                     SFPMPPLILALILGPLIEANYWTSILISYGDYLVFFKRPLSAIFMAVALLTFLWPWIS
                     ALRGKKAKDLS"
BASE COUNT          325 a          352 c          333 g          268 t
ORIGIN      
        1 gccagcgccg cgtaaaactt tccaaaaccg cagaaaacgg gaacggctat tgaataggag
       61 ttgggagaaa cctcggccat gctcgaagcg tacccacgct ccttcgtctc gacgatctga
      121 cggcggaacg cgtcggcgtc gtattctttc cgcattgtcg aaaaatgctc caagaacgct
      181 tctcgctgct cctccggaag aaatgcaaga agcgctttgc tcggcgctcc tttatacagc
      241 ggtcgctcct ctccttcgtt gatgacgtag cgaacggcgt tcctgctctc ttcgcgcaca
      301 agacaaagcc ttcggaagcc gtgaacgacg aagagcgagg cggtttcccc ggtttcctcg
      361 acaatctctt tcataaacga gttcaagagc actttgatgt tgagatacga cttcgaaaga
      421 cctccgccca acgaatgtac tttcggcccc agcgcatacc ggccggtcgc ctgatcctgc
      481 gtgacgtacc ccatatgccg caacgttcct gcaagacgca ttatcctggt cttgttgatt
      541 cccgtcctct cgtgaatatc gccgagagaa agagagggcg cggtctcgaa gcagtccagt
      601 atagtcagcg cgcttgcgac ggaatcaacg attttttccg actctttact ctccttaaac
      661 atatcatcac gccaagctca atggaatttt cgccgatcgc aactttcggc agtggaggca
      721 tcgcctcgcg aactagtttt gcggtacgca ctgttatggc ttacgataaa tccttcgctt
      781 tcttcccacg cagagcggat atccatggcc agaggaacgt cagaagggcg accgccataa
      841 agatcgcaga caaggggcgc ttgaagaaga caagataatc gccgtaggag atcagaatgg
      901 aggtccagta gttcgcctcg atcaggggtc cgaggatcag cgccaggatc aacggcggca
      961 tgggaaacga catctttccc aggacatacg cgactatgcc gacggccagc atgacataga
     1021 cattgaacat actgttctcg gaggcgaatg caccaagaaa gcagatcacc aggatcagtg
     1081 gcgctaaaat ctggacggga attcgaagga tagaataggc gatctgcaca aaaaagtgcc
     1141 cgatgaggaa cacgaggata ttcaccatag cgaaccccac gaagaacgta aagatcgttt
     1201 caggattgtt gcgcatcagc atcgggcccg gctggattcc cttcatgatg agcgctccaa
     1261 gaagaatcgc ggctgcgg
//
LOCUS       DAOSWW010000904         1275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_99280_length_1275_cov_4.709016, whole genome shotgun sequence.
ACCESSION   DAOSWW010000904 DAOSWW010000000
VERSION     DAOSWW010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99280_length_1275_cov_4.709016"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PK442_14100"
     CDS             complement(<1..363)
                     /locus_tag="PK442_14100"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOO88833.1"
                     /translation="MLRFLAGNVHSIIDNIVVLEVNGLGLELLCTGRAVALCAEGQDA
                     TLVTYLHFSEAGPTLFGFADDRERALFLKLTTVKGVGGKMAMNILKAAPAERIISWIL
                     SSDVDSMVKIQGIGKKTAE"
     gene            complement(341..868)
                     /gene="ruvC"
                     /locus_tag="PK442_14105"
     CDS             complement(341..868)
                     /gene="ruvC"
                     /locus_tag="PK442_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOO88834.1"
                     /translation="MLKCIGIDPGLGRMGVATVLQDGRSFRLISGGCIETSPGEEISS
                     RLDTIFRKLGEQIECCSPDFMSVEKLFFGRNITTAEYVWQARGIALLLAARYRLPLFE
                     PKPSQIKLTVCGYGTAPKEQVQKMVQRLLGLKEVPKPDDVADAIGAAITGFSLFAEKR
                     RISSAGGYHASLSRR"
     gene            complement(872..>1275)
                     /locus_tag="PK442_14110"
     CDS             complement(872..>1275)
                     /locus_tag="PK442_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO88835.1"
                     /translation="TRNGGSLGEAGSVAWMFERKGVIEVSGKDLDEEALMTVALEAGA
                     EDLQNQDDGFIVYSEPSVFGLVRDALEKAGYVVSRAETEMVPKNTVMIANKEKAQKIL
                     RLMDLIEEHDDVQNAYGNFDIPDEILESLDA"
BASE COUNT          305 a          382 c          283 g          305 t
ORIGIN      
        1 ctcggctgtc ttttttccta tcccctgaat cttgaccatg gagtcaacgt cagacgagag
       61 aatccacgaa atgatacgtt ctgcgggcgc agcttttaaa atattcatcg ccatcttccc
      121 gccgactccc ttcacggtgg tgagcttcaa aaaaagagcc ctctccctgt cgtctgcgaa
      181 tccaaagagc gtcggccccg cctccgagaa atgcagatac gtgacgagcg ttgcgtcttg
      241 gccttcagca caaagagcta cagcacgccc tgtacaaagg agttccaaac ccaaaccgtt
      301 gacttccagc acgactatat tgtcaattat cgagtgaaca ttacctgcga gaaagcgaag
      361 catgatagcc tcctgcactg gaaatacgcc gcttttccgc aaaaagcgaa aaaccggtaa
      421 ttgcagctcc tatagcatca gcgacgtcgt ccggcttggg aacttcttta agccccaaga
      481 ggcgctgtac cattttctgc acctgttcct tcggcgccgt tccatacccg cataccgtta
      541 acttgatctg agagggtttt ggttcaaaaa ggggtagacg gtaacgggct gcgaggagga
      601 gcgctatgcc gcgtgcctgc cacacgtact ccgcagtagt gatgttcctt ccgaaaaaaa
      661 gcttttcaac cgacataaaa tcaggggagc agcattcaat ctgctccccg agttttctga
      721 agatcgtgtc cagtcgggaa gagatttcct ctccgggcga tgtttcaata cacccgcccg
      781 agataagacg gaaagagcgt ccatcctgaa ggaccgttgc cactcccatc cggcccagcc
      841 ccgggtcgat cccgatacac ttcagcaacg cttacgcatc caggctttcc agaatttcat
      901 ccggaatgtc gaagttgccg taggcattct ggacgtcgtc gtgttcttcg ataagatcca
      961 taagacgcaa aatcttctgc gctttttcct tatttgctat cataacagta ttcttcggga
     1021 ccatctcggt ctcggcccgg ctgacaacat agcctgcttt ctccaaggcg tcccgcacca
     1081 gaccaaaaac actcggctca ctgtatacaa taaagccgtc gtcctgattc tgcaggtctt
     1141 ccgctccagc ctccagggca actgtcatca aggcctcttc gtcaagatct ttccctgaaa
     1201 cttcaatgac gcctttccgc tcgaacatcc acgcaacgct tcctgcttca ccaagggaac
     1261 cgccgtttct tgtca
//
LOCUS       DAOSWW010000905         1274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_99419_length_1274_cov_3.326497, whole genome shotgun sequence.
ACCESSION   DAOSWW010000905 DAOSWW010000000
VERSION     DAOSWW010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99419_length_1274_cov_3.326497"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK442_14115"
     CDS             complement(<1..285)
                     /locus_tag="PK442_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOO88836.1"
                     /translation="MIVQKYGGSSVATPEKIKNVAARIKKRVDEGEKLIVVVSAMGKT
                     TNSLISLAQEVSAEPSPRELDMLLATGEQMTSALLAMALSDFGIPALSYNA"
     gene            complement(320..1021)
                     /gene="dapD"
                     /locus_tag="PK442_14120"
     CDS             complement(320..1021)
                     /gene="dapD"
                     /locus_tag="PK442_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HOO88837.1"
                     /translation="MRTEEIIRLIKESKKKTPAKAYIAGDLEGLDGERITFIGGKDFG
                     ILLGDRDDVERVLVENAGKIRSAHVEISARNSAVPLADLTKYEARIEPGAIIRDMVEV
                     RKGAVVMMGAVINIGAVIGERTMIDMNVVVGGRAEIGADCHIGAGAVVAGVIEPPSAT
                     PVVIEDNVLVGANAVILEGVRVGKGAVVAAGAIVTKDVPPHSVVAGSPAKVIKEVDAK
                     TESKTQIVQDLRTLK"
     gene            complement(1003..>1274)
                     /locus_tag="PK442_14125"
     CDS             complement(1003..>1274)
                     /locus_tag="PK442_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOO88838.1"
                     /translation="KKKDAPSGTAILLREATGRDCPTHSLRLGGIPGDHTVCYANEGE
                     VLTLTHRALSRSVFALGALKAAEFAVSAAPGFYSFEEVLSCGLKR"
BASE COUNT          205 a          453 c          362 g          254 t
ORIGIN      
        1 cgcgttgtag gagagcgccg gaattccaaa gtcgctcaac gccatcgcca gaagagcgga
       61 ggtcatctgc tctccggtgg cgaggagcat gtccagctca cggggcgacg gctccgccga
      121 cacctcctgc gcgagggaga tgagcgaatt cgtggtcttt cccatcgcgg agactaccac
      181 gatgagcttc tccccctcgt ccacgcgctt ttttatcctg gcggcgacgt tctttatctt
      241 ctcgggcgtg gccaccgacg acccgccgta cttctgcacg atcacttccg cttctctccc
      301 cttcgtccga ccggttcgat cacttgagcg tccggaggtc ctgaacgatc tgcgtcttcg
      361 actccgtctt ggcgtccact tccttgatca ccttcgcggg cgatccggcg accacggaat
      421 gcggcggcac gtccttcgtc acgatggctc cggccgccac aacggcgccc tttccgaccc
      481 gcactccttc cagaatgacc gcgttcgcgc cgacgagcac gttatcttcg ataaccacgg
      541 gcgttgcgct cgggggctcg atcactccgg caaccaccgc gcctgcgccg atgtggcagt
      601 ccgcgccgat ctccgcccgt cctccgacca cgacgttcat atcgatcatg gtccgttcgc
      661 cgatgaccgc gccgatgttg atcaccgcgc ccatcatcac cacagcgcct ttgcggacct
      721 ccaccatgtc gcggataatc gcgccgggct cgatgcgcgc ctcgtacttc gtcaggtcgg
      781 cgaggggcac ggccgagttg cgggccgaaa tctcaacgtg cgcggagcga atcttccccg
      841 cgttctccac gagcacccgc tccacgtcgt cacggtcgcc gagcagaatg ccgaagtcct
      901 ttcctccgat gaaggtgatc cgctcgccgt caagtccctc aagatccccg gcgatgtacg
      961 ccttggcggg cgttttcttc ttcgactcct tgatgagccg tattatctct tcagtccgca
     1021 tgagaggacc tcctcgaaag aatagaatcc gggcgcggcc gagaccgcaa actctgcggc
     1081 cttcagcgcg ccgagggcga agacgctccg cgaaagagcc cggtgcgtca gggtaaggac
     1141 ctcgccctcg ttcgcgtagc agaccgtatg gtctccggga atccccccga ggcgcagcga
     1201 atgcgtcggg cagtcgcgcc ccgtcgcctc cctgaggaga atcgccgtcc ccgagggggc
     1261 gtccttcttc ttgt
//
LOCUS       DAOSWW010000906         1274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_99469_length_1274_cov_2.367514, whole genome shotgun sequence.
ACCESSION   DAOSWW010000906 DAOSWW010000000
VERSION     DAOSWW010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99469_length_1274_cov_2.367514"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK442_14130"
     CDS             complement(<1..193)
                     /locus_tag="PK442_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88839.1"
                     /translation="MPHEITAFFRYLVFSLRPLGIVLDVPTLLASFNEYEKRWMLSLN
                     WRESLGEEEGAGEAETADVY"
     gene            complement(183..494)
                     /locus_tag="PK442_14135"
     CDS             complement(183..494)
                     /locus_tag="PK442_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88840.1"
                     /translation="MNKDSLSATRILRLNRLVQQLQACGKDGTPVGSDALVRDAGYHA
                     QCSSHETAVRLLQRDIQFLRDSFFTEITYDRVRKGYTLESLGSYVLFSFLGVPATSIA
                     A"
     gene            complement(574..819)
                     /locus_tag="PK442_14140"
     CDS             complement(574..819)
                     /locus_tag="PK442_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88841.1"
                     /translation="MSKDISSVRIVRLNSLLAQWGLRRRGCPIPTGILIREVGYGGET
                     KKEEASIRLLQRDIRFFKDHFSLEIVYDRRRKGYVLQ"
     gene            complement(946..>1274)
                     /locus_tag="PK442_14145"
     CDS             complement(946..>1274)
                     /locus_tag="PK442_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88842.1"
                     /translation="EARTPPWVASQSCLVLRIRNADAVPPEYLYMYLRSDLGKSLLKV
                     LTSGMLVPLIHLRQLSGIPVVIPSAEKKRQALESYLRQSELERSIAEARNEQSRLLDA
                     LFSGGD"
BASE COUNT          264 a          372 c          358 g          280 t
ORIGIN      
        1 cgtacacatc agctgtttcc gcttcaccag cgccttcctc ctcccccaag gactcccgcc
       61 agttcaagga taacatccac ctcttctcat attcgttgaa ggaggcgagg agtgtcggaa
      121 catcgagcac gatgccaagg ggacgcagtg agaaaaccag ataccggaaa aacgcggtta
      181 tttcatgcgg caatggaggt ggcgggaacg ccgagaaagg agaagagcac atagctcccg
      241 agggattcca gagtatagcc tttgcgcact ctgtcgtatg tgatctcggt gaaaaatgag
      301 tctctgagaa actgtatatc ccgctgaagc aaacggacgg ccgtttcgtg gctactgcac
      361 tgcgcatggt acccagcgtc gcggacgagc gcgtccgatc cgaccggggt tccgtccttt
      421 ccacacgcct gaagctgctg cacgagcctg ttgagacgca gaattcttgt tgccgacagg
      481 gagtccttgt tcatctcgat cgacctcctc gcgtcgtctc ggggtgtacg gcggcgtttc
      541 cgccgccgcg tccggtcata caggagtgaa gaactactgc agtacgtatc cttttcgtct
      601 tctgtcgtac acgatttcca gggagaagtg atccttgaaa aaacgaatgt ctctttgcag
      661 cagtcgtatc gaggcctctt ccttcttcgt ttccccgccg taccccacct ccctgatgag
      721 gatccccgtc ggaatgggac agccgcgtct ccgcagcccc cattgcgcca gcaaactgtt
      781 caaacgcacg atccggacag acgatatgtc tttgctcatc ggtatcgcat cctttctttc
      841 gcatgccttc ggggtacggg ctacgccttg ttcttaagga gcggtgcgat gacagctttt
      901 gcgagtatcc gctatccgga acgcgactgc gtgaacgccg tcttttcaat cacctccgga
      961 aaaaagagcg tcaagcagac ggctctgctc gtttcgcgct tccgcgatac ttcgttcgag
     1021 ttcggactgc cgaaggtagg attccagagc ctgccgcttc ttttccgccg aagggatgac
     1081 gacgggtatg ccgctcagct ggcggagatg gatcaaaggc accagcatcc ccgaggtgag
     1141 caccttcagc agcgatttgc caagatcgga acgcaggtac atgtacaggt actcgggagg
     1201 aacggcgtcc gcgttcctga tccggaggac gaggcaggac tggcttgcga cccagggcgg
     1261 cgtgcgcgct tcgg
//
LOCUS       DAOSWW010000907         1271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_99811_length_1271_cov_3.407895, whole genome shotgun sequence.
ACCESSION   DAOSWW010000907 DAOSWW010000000
VERSION     DAOSWW010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99811_length_1271_cov_3.407895"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PK442_14150"
     CDS             <1..720
                     /locus_tag="PK442_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO88843.1"
                     /translation="NLLTRMFTLEYSYWFDYMLPGIEALGMPIPLGGTSNHFRTEMLR
                     LLGGWDPFNVTEDADLGIRASAHRMWVSTINSTTFEEANSHTGNWIRQRSRWIKGYMQ
                     TYLVHMRNPLRLFRTIGLMPFLGVQLLIGGTFMTFLINPILWLLFIFWIAFRPLWMES
                     VFPLWILTLSTLNLIVGNAMGIYLNMLAVFRRRLFHLTFYSLMNPFYWILHSIAAYKA
                     LWQLFTKPFFWEKTVHGLAKK"
     gene            701..>1271
                     /locus_tag="PK442_14155"
     CDS             701..>1271
                     /locus_tag="PK442_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88844.1"
                     /translation="MDSRRSRRIFLFCAALLSFALLFLVARGVFVRFFLPEAASVSVT
                     VLEASRFEPVELLLAQPTLVALIAWLSPFRSFGVLWPSVCAGAGLLLAIALLYPLRAS
                     SHPFFSLLFFVPAVLSAPFLFMLTADPASAIAVACLSVAVADLLSHLRTGRTFPLFSA
                     GLALGLVPFAASWGLYAVLGAVIVNRLLIE"
BASE COUNT          180 a          401 c          348 g          342 t
ORIGIN      
        1 aatctgttga cgaggatgtt cacgctggag tactcctact ggttcgacta tatgctcccc
       61 ggaatcgagg cgctggggat gcccattccg ctcggcggca cgtcgaatca cttccggacg
      121 gagatgctcc gcctgcttgg agggtgggac cccttcaacg tgacggagga cgccgatctt
      181 ggaatacggg cgagcgccca caggatgtgg gtctccacca tcaattcgac caccttcgaa
      241 gaagcgaaca gccataccgg caactggatc cggcagcgtt cccggtggat caaggggtac
      301 atgcagacgt atctggtgca catgcgcaac ccgctacggc tcttccggac gatcggtctc
      361 atgccatttc tgggcgttca gctcctgatc ggaggaacct tcatgacgtt cctcatcaat
      421 ccgatcctct ggctgctttt tattttctgg atagccttcc gtcccctgtg gatggagtcg
      481 gtcttcccgc tctggatttt gaccctctcc acgctgaacc tgatcgtcgg caacgccatg
      541 gggatttacc tgaacatgct cgcggtcttc cgccgcagac tcttccatct gacattctac
      601 tcgctcatga atccgttcta ctggattttg cactcgattg ccgcgtacaa ggcgctctgg
      661 cagcttttta caaaaccgtt cttctgggag aagacagtcc atggactcgc gaagaagtag
      721 acggattttt ctattctgcg ccgcgcttct ctccttcgcg ctgctctttc tcgttgcgcg
      781 gggtgtcttc gtgcgtttct tcctgcctga agccgcctca gtctccgtca cggtgctgga
      841 agcgtcccga tttgaaccgg tcgagctttt gctggcgcag ccgactcttg tagcgctgat
      901 cgcatggctc tcgccgttcc gctcgttcgg ggttctctgg ccgtccgtat gcgcgggggc
      961 tggcctcctg ctcgcaatcg ccctccttta tcctctgcgc gcgtcttcgc accctttctt
     1021 ttctttgctc ttcttcgttc cggcggttct ttcggcgccg ttcctcttca tgctgacggc
     1081 ggatcccgcg tctgcgattg cggtcgcctg tctctcggta gccgtcgccg atttgctttc
     1141 tcacttgcgt acggggcgca cgtttccgct cttcagcgcc gggctggcgc tcggtctcgt
     1201 tcccttcgcc gcttcgtggg ggctgtatgc cgtcctggga gccgtgatcg tgaaccggct
     1261 cctgatcgaa g
//
LOCUS       DAOSWW010000908         1269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_100039_length_1269_cov_3.135091, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000908 DAOSWW010000000
VERSION     DAOSWW010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100039_length_1269_cov_3.135091"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PK442_14160"
     CDS             complement(<1..558)
                     /locus_tag="PK442_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO88845.1"
                     /translation="MEKNDKYALEMRNVSKAFPGVQALRDVTLQVRPGTVHALMGENG
                     AGKSTLMKCLFGLYSLDEGEVVLEGKPVSISSSREALDLGISMIHQELHPIPERSVME
                     NIWLGRAPLRKIGGVRFIDRKSMEAATGKLMKELELDINPRTLVRNLSVSKIQSMELA
                     KAVSYNARIIVMDEPTSSLTGHEVSH"
     gene            complement(680..>1269)
                     /locus_tag="PK442_14165"
     CDS             complement(680..>1269)
                     /locus_tag="PK442_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO88846.1"
                     /translation="MSGQIIVDFWKSHPEADKNGDGVLQYVMLKGEPGHQDAELRTEF
                     SIKAVTDAGIKVEKLAEDTGLWDRVRGQEKMAAFLAAHGDKIEAVFGNNDDMALGAIE
                     ALKAAGYFSDGKFMPVVGVDATAPAIQALEDKTLLGTVLNDAVNQGVATFNLSYILAK
                     GETPTEENSGYVISDGKYVWVPYRKVTLENVAEFK"
BASE COUNT          235 a          413 c          332 g          289 t
ORIGIN      
        1 gtggctgacc tcgtgcccgg tgagcgagga ggtcggttcg tccatgacga tgatccgggc
       61 gttgtacgat accgccttcg ccagctccat cgactggatc ttcgacacgg aaaggttgcg
      121 tacgagagtg cgcggattga tgtccagctc aagctccttc atcagcttcc cggtcgccgc
      181 ttccatggac ttcctgtcga tgaagcgtac ccccccgatc ttccgcagag gagcgcgtcc
      241 gagccatata ttttccatga ccgaacgttc cggtatcgga tggagctcct gatgaatcat
      301 cgagataccc aggtcaagcg cctcacgcga acttgatatc gagacaggct tcccttccag
      361 gaccacctcc ccttcgtcga gagaatacag gccgaagagg cacttcataa gcgtcgactt
      421 tcctgcgcca ttctctccca tcagcgcatg aacggtcccc gggcgcactt gcagcgtgac
      481 gtcgcgaagc gcctgcaccc ccggaaatgc ctttgaaaca ttccgcatct caagtgcata
      541 cttgtcgttt ttttccacga agacaccttc ccttcgcatg cctctatcgc tcccggagca
      601 caatgccagg gaacgatagc aaagaaaggg aggcgcttgc ttgcccccct ttcaaagctg
      661 cctttccgca aacgacgttc tatttgaact ccgcgacgtt ctccagagtg acctttctgt
      721 agggaaccca gacgtacttc ccgtcgctga tgacgtatcc gctgttctcc tcggtcgggg
      781 tctcgccctt cgccaaaatg taggaaagat tgaaggtcgc gactccctgg ttgactgcgt
      841 cgttcagcac tgtgcccagg agcgtcttgt cctcaagggc ctgaatcgcc ggcgcggtcg
      901 cgtcgacgcc aacaacgggc ataaactttc cgtcgctgaa gtagcctgcc gctttcaggg
      961 cttcgatcgc cccaagcgcc atgtcgtcgt tattgccgaa gaccgcttcg atcttgtcgc
     1021 cgtgcgccgc gaggaaggcc gccatcttct cctgaccgcg gaccctgtcc cacagccccg
     1081 tatcctccgc gagcttctcg accttgattc ccgcgtcggt taccgccttg atggagaact
     1141 cggtacggag ctcggcatcc tgatgccccg gctctccctt gagcatgacg tactgaagga
     1201 cgccgtcgcc gtttttgtcc gcttcggggt ggctcttcca gaagtcgacg atgatctgcc
     1261 ccgacattg
//
LOCUS       DAOSWW010000909         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_100599_length_1265_cov_3.918182, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000909 DAOSWW010000000
VERSION     DAOSWW010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100599_length_1265_cov_3.918182"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK442_14170"
     CDS             <1..293
                     /locus_tag="PK442_14170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO88847.1"
                     /translation="EFLLVLRMKAGGDYMAEYAAICAGIAATPLKTRIGEIPITVSIG
                     VSCAKGKSGVDAILGLADAALYSAKGLGRNRVVFAGGENAGEAGAACCPRSE"
     gene            complement(345..>1265)
                     /locus_tag="PK442_14175"
     CDS             complement(345..>1265)
                     /locus_tag="PK442_14175"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HOO88848.1"
                     /translation="QKIRFFNRFYTRIIGAVNQSVLNSPFSMAEVRVLFEIKHSDNPT
                     ASEVNRELELDPGYLSRIVRRFEQDELLTKERSHLDGRAYLLKLTEHGERVLASLEKA
                     SDKKAADLISPLSEREITALLRSMETIHSLLTKTPLELEVRPAAPGELGTIVARHMDY
                     YSKQYAFDASFESYLFEGIARFLKNRENGMGQAWVVKHGGVVVGSIAIDHVEDRVAQL
                     RWFLVEPEYRGMGMGRRLIEEALDFSRMNLYHRIFLWTFSELRNARHLYEMYGFSPTE
                     EVTHTIWGRELTEERWDILLFDPDEEVEMV"
BASE COUNT          276 a          351 c          363 g          275 t
ORIGIN      
        1 aggaatttct gcttgtcctc cggatgaaag cggggggcga ttacatggcg gaatacgccg
       61 cgatctgcgc cgggatagcg gcgacgcctc tgaagacgag aataggggaa attccgatca
      121 cagtcagtat cggcgtttca tgtgcaaaag ggaagagcgg agtggacgcc attctgggtc
      181 tggcggacgc cgccctctac agcgcgaagg gcctcggacg gaacagagtc gtattcgccg
      241 ggggggaaaa cgccggagaa gccggcgccg cttgttgtcc tcgctcggaa taaggccgcc
      301 ggagaaagag aagagccttc ccgtacggaa aggctctcca gatgctagac catttcaacc
      361 tcttcgtcag ggtcgaagag gaggatatcc caccgttctt cggtcagttc acgcccccaa
      421 attgtgtgcg tgacctcctc agtgggggag aatccgtaca tctcgtagag atggcgtgcg
      481 ttccgaagtt cggaaaacgt ccacaggaag atcctgtgat acaggttcat ccggctgaaa
      541 tccaaagcct cttcgatgag gcgcctcccc attcccatcc cgcggtactc cggttctacg
      601 aggaaccagc ggagctgggc aacccgatct tcgacatggt ctatcgcgat ggagccgaca
      661 accacaccgc cgtgcttcac aacccaggcc tgccccatgc cgttctccct gttcttcagg
      721 aaacgggcta ttccttcgaa gaggtaggac tcaaacgacg cgtcgaatgc gtactgtttg
      781 ctgtaatagt ccatgtgacg cgcaacgatc gtgccgagtt cgcccggagc tgcaggccgg
      841 acttcgagct caagcggagt tttcgttaaa agtgagtgga tggtctccat agagcgcagc
      901 aacgctgtga tttctctctc tgaaaggggg gagataagat ccgccgcttt cttgtccgac
      961 gccttttcga gagaggcgag cacacgttcg ccatgttcgg tcagcttgag cagatacgct
     1021 cttccatcga gatgcgaacg ctccttcgtc agcagttcat cctgttcaaa gcggcggact
     1081 atcctgctca agtaccccgg gtcgagttcg agctccctgt tgacttcgct tgccgtaggg
     1141 ttatcggagt gttttatctc gaagagcact ctcacctccg ccatggagaa tggactgttc
     1201 aacaccgact ggttgacggc gccgatgatc cgtgtataaa acctgttgaa aaaacgtatc
     1261 ttctg
//
LOCUS       DAOSWW010000910         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_100602_length_1265_cov_3.637190, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000910 DAOSWW010000000
VERSION     DAOSWW010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100602_length_1265_cov_3.637190"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PK442_14180"
     CDS             complement(<1..443)
                     /locus_tag="PK442_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88849.1"
                     /translation="MLPPTKSVSIKDIILLAFVMLTLITGGISGFIVFSGWLSSAEEN
                     AGRMADEISDVIFSRVNAHFNIPLHINAAYREQLEKGVIDMNNPLQRERFLASVMRAH
                     SGEVIYSFGYATTEGEYYAVRWNERNELEVARNNSETGGHSFYYSV"
     gene            complement(590..>1265)
                     /locus_tag="PK442_14185"
     CDS             complement(590..>1265)
                     /locus_tag="PK442_14185"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOO88850.1"
                     /translation="SAFGFPPNLVAASVDEDALRLSSRTSPKPVEGLLYRYEALPGVS
                     PPVVPNAVLKYSPELFRNGDIVFVNGKRWRTKIVRLFDKSPRGFAHAGIVRIVDGLPH
                     VVHASPELGKVGMQSMEDFFSFENVDYARVYRFSGDPAVAEAASLRALEYCTREIPFD
                     DRFDTSRTEAMYCTEVIWRAYKAEGIDLARGNWEFARNPIVQGRVLLPYKLSLSPHLE
                     EAFVLE"
BASE COUNT          258 a          371 c          378 g          258 t
ORIGIN      
        1 acggaataat agaaggagtg tccgccggtc tccgagttgt tccgcgcgac ctccagctcg
       61 ttccgttcat tccaccggac ggcgtagtat tccccctccg ttgtggcgta gccgaagctg
      121 tagataacct cgcccgaatg cgcgcgcatc acggaagcca gaaagcgctc ccgctgcagc
      181 ggattgttca tgtcgatgac gcctttttcc agctgttcgc ggtaggcggc gttgatatgc
      241 aggggaatgt tgaaatgcgc gttcaccctg ctgaagatga cgtcgcttat ctcgtcggcc
      301 atccggccgg cgttctcctc ggcggacgag agccatccgg agaagacgat gaagcccgaa
      361 atcccgccgg tgatcagcgt cagcatcacg aacgccaaaa ggatgatgtc ttttattgag
      421 acgctcttcg tcggcggaag catgggctca cctcgatcat tccgggctct tcattatgaa
      481 ggcagttgta attatacaga caaaattccc ttttattcaa ccgctcgttc cgtcgtgacc
      541 gtgatactgt gtaaatgtgt attcttgcag ttcgaaaaag gacgaggcgc tactcgagca
      601 cgaacgcttc ctccagatgc gggctcagac tcagtttgta gggaaggagc accctcccct
      661 ggacgatggg gttgcgcgcg aactcccagt ttccccgggc cagatcgatc ccctccgcct
      721 tgtaggcgcg ccagatcacc tcggtgcagt acatggcttc tgttctcgac gtatcgaacc
      781 ggtcgtcgaa ggggatttcg cgcgtacagt attcgagcgc ccgcaggctc gccgcctccg
      841 cgacggcggg atcgccggag aagcggtata cccgcgcgta gtccacattc tcgaaggaga
      901 agaagtcctc catcgactgc atccccacct tgcccagctc gggcgacgcg tggacgacat
      961 gcggaagacc gtcgacgatg cgcacgattc cggcgtgagc gaatccgcgg gggcttttgt
     1021 cgaagaggcg gacaatcttc gttctccacc tcttgccgtt gacgaagacg atgtccccgt
     1081 tgcggaagag ttccggcgaa tacttcagaa ccgcattcgg cacgacggga ggagatactc
     1141 ccgggagcgc ctcgtaacgg tagagcagcc cttcgacggg cttcggagaa gtccgggagg
     1201 agagtcggag cgcgtcctcg tccacggagg cggcgacaag attcggcgga aagccgaaag
     1261 cggag
//
LOCUS       DAOSWW010000911         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_100611_length_1265_cov_3.274380, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000911 DAOSWW010000000
VERSION     DAOSWW010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100611_length_1265_cov_3.274380"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PK442_14190"
     CDS             complement(<1..1090)
                     /locus_tag="PK442_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO88851.1"
                     /translation="MNGAYGGGLHVYTMLDPDLQEAGERIAARSKHQLAIVGLDSETG
                     GVRALVGGKDWHVSQFDRAVQAYRQPGSAFKPLVYATLFEKKGWSGATKIQDAPISFG
                     SGRNRWSPRNYDGKFRGSVTVERAIIGSLNVPAVRALSAVGAGVVAETVRSMGITTPY
                     LPDNPTMALGSASLTPLEMAAAFSVFGNGGYRIVPTLVREIRDADGKVLFKAAEEKKR
                     VLSEYTANGIRDILIKAVSSGTGRAARIDGFQVFGKTGTTNDFRDAWFVGGIPGFCAA
                     VYVGDDDRKPLGKGATGGKIAAPIWQDFMEYAADTLCAPAAFPKNTEKTPPVPRERGT
                     QETTDPVKESETASPAPPKETPTVAPPQA"
BASE COUNT          198 a          449 c          380 g          238 t
ORIGIN      
        1 ccgcctgagg cggagcgacc gttggcgtct ccttcggagg ggccggagac gccgtctcgg
       61 actccttaac ggggtccgtc gtttcctgcg taccgcgctc ccgcggaaca ggaggcgtct
      121 tttccgtgtt cttcgggaac gcggccgggg cgcagagcgt gtccgcggcg tactccataa
      181 agtcctgcca gataggggcc gcgatctttc cgccggtagc gccctttccg agcggcttcc
      241 tgtcgtcgtc gcccacgtag acggcggcgc agaagcccgg aatgccgccg acgaaccatg
      301 cgtcccgaaa gtcgttcgtc gtccccgtct tgccgaagac ctggaatccg tcgatgcgcg
      361 cggcgcgtcc cgttccggac gaaacggcct tgatgagaat gtccctgatc ccgttcgcgg
      421 tgtattcgga gaggacgcgc ttcttttcct ccgccgcttt gaagaggacc tttccgtcgg
      481 cgtcgcggat ctcgcggaca agcgtcggga cgattctgta ccctccgttc ccgaagaccg
      541 agaaggccgc cgccatttcg agcggcgtca gcgaggcgct ccccagcgcc atcgtcggat
      601 tgtccggaag gtacggcgtg gtgatgccca tgctgcggac ggtttccgcc acaactcccg
      661 cccccacggc gctcaacgcc cggaccgccg ggacgttcaa cgacccgatg atcgcccgct
      721 cgaccgtcac gctcccccgg aacttcccgt cgtagtttct gggcgaccat ctgttccgcc
      781 ccgagccgaa cgaaatcgga gcgtcctgaa tcttcgtcgc cccgctccac ccctttttct
      841 cgaagagcgt agcgtacaca agcggtttga aggccgatcc gggctgccga tacgcctgga
      901 cggccctgtc gaactgactt acatgccagt ccttgccgcc gacgagcgcc ctgacgcctc
      961 cggtctccga gtccagcccc acgatcgcga gctggtgttt cgacctcgcg gcaatgcgtt
     1021 cgcccgcctc ctgcagatcg gggtcgagca tcgtgtagac gtggagtcct cctccgtacg
     1081 ccccgttcac cccgtactac gcgatgcggg tgctcaacga cacgctgctg cccgagtacg
     1141 gataccgcac atgctccgaa ggaaggttcc ccggctcagg aacgagcgcg acagaagctt
     1201 ccgcgtcgga acgctcctgc gcggagatta cgccggtttc gaacatcctc gccagcacgc
     1261 gtctc
//
LOCUS       DAOSWW010000912         1262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_101065_length_1262_cov_2.996686, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000912 DAOSWW010000000
VERSION     DAOSWW010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101065_length_1262_cov_2.996686"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1262
                     /locus_tag="PK442_14195"
     CDS             <1..>1262
                     /locus_tag="PK442_14195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOO88852.1"
                     /translation="AVEHIDYDAFRGNVDLAAGVRRPLGVRPNPVVWSKISGKVLLEG
                     AEQPLPGVRVSIAPKSVKATLQGPVEFMTGWDGAFVVSGLPPGVYKVELSHSGCAPKS
                     FDLQVAPNMQEVSWSLAPLFDTVSLTVTVRDGDGGKEIPGARVVLAEAWPRGEIAQKT
                     TDASGSALFEGLKIGRLNRAGADGRMTLSRSAVTVRAEADGYEPMLVPVLLGRTASVE
                     VQLNSAEKIAEQPGNLDFSSAQRIRTGAPVEFVIENAGENKFFRFHLPYPAWVGLDAN
                     RGQNPWLRFHLHAADGTEIGNWGLSGESALFDAGALPAGEYVLQAREWNNNKAAALPM
                     TFTIQCRWASDPHSPNAELSSARAFRTGEEVRGYILPRGDRNHFRFEIKRPGQVRLWL
                     QRPENIWIRAFLFRSDGAEIANLGCSAAT"
BASE COUNT          220 a          395 c          430 g          217 t
ORIGIN      
        1 gccgttgaac acatcgacta cgacgccttc cggggaaatg tcgatctggc ggccggagtg
       61 cggcgtcctc tcggcgtcag gcccaatccg gtcgtctgga gcaagatctc ggggaaggtg
      121 cttcttgaag gtgcggagca gcccttgccg ggcgtgcgcg tctccatcgc tccgaagagc
      181 gtgaaagcaa cgctccaggg gcctgtggag ttcatgaccg gctgggacgg cgccttcgtc
      241 gtgtccgggc ttccgcccgg cgtctacaaa gtcgagttga gccattccgg atgcgcgccg
      301 aagtccttcg acctccaggt cgcgccgaat atgcaggagg tctcgtggtc gcttgcgccg
      361 ctcttcgata cggtctcgct caccgtgacc gtaagggacg gcgacggagg taaggagatc
      421 ccgggcgcgc gcgtcgttct cgccgaggcg tggccccggg gagagatcgc ccagaagacg
      481 acggacgcga gtggatcggc gctcttcgaa gggctcaaga tcgggcggct caaccgcgca
      541 ggcgccgacg gcaggatgac tctcagcagg agcgccgtca cagtgcgcgc cgaagccgac
      601 gggtacgagc cgatgcttgt ccccgtcctt ctcggtcgga cggcgtccgt ggaagtgcag
      661 ctcaactcgg cggagaagat cgctgaacag cccggcaatc tcgatttttc ctcggcgcag
      721 cggatcagga ccggcgctcc agtagagttt gtcatcgaaa acgcgggaga gaacaagttc
      781 ttccgcttcc atctgccgta ccccgcatgg gtcggactgg acgcgaacag aggacagaat
      841 ccgtggttac gcttccatct gcacgcggcg gacggaacgg agatcggcaa ctgggggctc
      901 tccggagaga gcgcgctctt cgacgccggg gcgcttccgg caggcgagta cgtgctccag
      961 gcccgggagt ggaacaacaa caaggcggcg gcgttgccca tgacctttac gatccagtgc
     1021 cgctgggcct ccgacccgca ttctccgaac gcggagctct cctcggcacg ggctttccgg
     1081 acgggcgagg aggttcgagg gtatatcctc ccgcgcgggg accggaatca cttccggttc
     1141 gagatcaagc ggcccggcca ggtacgcctc tggctccaac ggccggagaa tatctggata
     1201 cgcgcctttc tgttccggtc cgacggcgcc gagatagcga acctcggctg ttcggcggcg
     1261 ac
//
LOCUS       DAOSWW010000913         1259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_101464_length_1259_cov_4.392027, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000913 DAOSWW010000000
VERSION     DAOSWW010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101464_length_1259_cov_4.392027"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /gene="whiA"
                     /locus_tag="PK442_14200"
     CDS             <1..432
                     /gene="whiA"
                     /locus_tag="PK442_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOO88853.1"
                     /translation="GFTAGRRKIQGKYEVTLRNQQEIADILASFNMVKTMLHLEEKAI
                     FRSLRNQANKLVNCDASNIRKSLEASSRQIEISRNALELPDYDDFPAVLKDLISTRLA
                     NPSMTLEELGRIQSPPVSKSTIKYRWKKIEDLATPLWQRRK"
     gene            530..>1259
                     /locus_tag="PK442_14205"
     CDS             530..>1259
                     /locus_tag="PK442_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOO88854.1"
                     /translation="MKLRTFSESDVRGKNVLVRVDFNVPLKNGKVTDSTRILAHRETV
                     STLLGWGAQVALSSHLGRPKGKRVPEMSLECVLSEAEKAFRVPTRFCNDCIGPSVQSA
                     VDGMKAGELLLLENLRFYAEEQENDTAFAGKLAAPFDVFVMDAFSASHRADASTNAIM
                     GILPSFSGALLRREVEMLSAVSEKPESPFVLILGGAKVSDKIGVVEHLMDKTSTILIG
                     GGMAFTFLKAAGKPIGRSLFEAEKK"
BASE COUNT          353 a          300 c          323 g          283 t
ORIGIN      
        1 ggatttacag ctggaagaag aaaaattcag gggaaatatg aagtcactct cagaaaccag
       61 caggagattg cagacatact tgcgagtttc aacatggtaa agacaatgct ccatcttgag
      121 gaaaaggcga tcttccgatc gttgagaaac caggcaaaca agctggtgaa ctgtgacgcc
      181 tcgaatattc ggaagagcct ggaggcatcg tctcgtcaga tcgaaatatc gcgcaacgca
      241 ctcgaactcc ctgattacga tgatttccca gccgttctga aagaccttat atccacacgt
      301 ctggccaatc caagtatgac gttggaagaa ctcggccgta tccagtctcc ccctgtaagc
      361 aaaagtacta taaaatatcg ctggaaaaaa atagaagacc tggcaacccc tttatggcag
      421 cggcgaaaat aaaatttctc taacttcaga tcccgcgatg ccaaagtgaa ggggtacgcc
      481 atataataga aatgctcacg acaaagaagc taaaggaggt cggaaacaca tgaaactgcg
      541 tacgttttca gaaagcgatg tccgtgggaa aaatgtgctt gtgcgggttg atttcaatgt
      601 tccgttgaaa aatgggaaag taacggattc cacgcgaatc cttgcccaca gggaaactgt
      661 ttctacgctt ctgggatggg gagcccaggt tgcgctctct tcccatcttg gccgaccaaa
      721 agggaaaaga gttcccgaaa tgtctctcga atgcgttttg tcggaagcgg aaaaggcgtt
      781 ccgtgttcca accagatttt gcaacgactg cattggacct tcagtccaat ctgcagttga
      841 cggaatgaaa gcgggggagt tgctcctgct cgaaaacctt cgtttttacg cggaagaaca
      901 agaaaatgac acggcgttcg ccggcaagct cgccgctccc tttgatgtct tcgtcatgga
      961 cgcgttcagc gcctcgcaca gagcagacgc gtcgacaaac gctattatgg ggattctccc
     1021 ctccttttca ggcgctctgc ttcgtagaga agtggaaatg ctgtcagccg tgagcgagaa
     1081 gcctgaatct ccgttcgtcc ttatcctcgg aggcgcaaag gtttcggata aaatcggcgt
     1141 ggtcgagcat ctcatggata agacaagtac gatactcatc ggaggcggta tggccttcac
     1201 gttccttaaa gcagccggga aaccgattgg acggtccctt ttcgaggcgg aaaagaaag
//
LOCUS       DAOSWW010000914         1258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_101637_length_1258_cov_4.138820, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000914 DAOSWW010000000
VERSION     DAOSWW010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101637_length_1258_cov_4.138820"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..994
                     /gene="dctP"
                     /locus_tag="PK442_14210"
     CDS             8..994
                     /gene="dctP"
                     /locus_tag="PK442_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO88855.1"
                     /translation="MLVSALVVLACLVRPAAAAPETVLRFAGQFPAEHTSTKLMQQLA
                     DEVKEKTGGRIEIRLHPDNELGDYTLIFEELIRGSIDMAAITVPSQFDPRLELCNING
                     FVRTFDEARTVFAPDGWVFKKMDELNSALGVKLLGFQFEGFIGIGSTKPVNEPLNPEV
                     KKGILLRIPNMEVFKLGAEAMGYRTITIPWDEVHGSLQNGFAEGVYGMTPAAAYTILK
                     DVIKYWYDLRHSVENLNYMMSLKTWEKLSEEDRGIIQNACAKISAVSVYLAEKDQEKY
                     LQSMRDAGIEVHTYTPEELAPLFVKVSATWDKLAERFSQELIDEFKKEFARQ"
BASE COUNT          278 a          346 c          361 g          273 t
ORIGIN      
        1 gtatttcttg cttgtttccg cgcttgttgt tcttgcctgc cttgtccgcc ccgccgcggc
       61 cgccccggag acggtgctcc gtttcgcggg acagtttcct gcggagcaca cgtcgacgaa
      121 gctgatgcag cagctggccg acgaggtgaa ggagaagacc ggcggacgaa tagagatcag
      181 gctccacccc gacaacgaac ttggcgacta tacgctcatt tttgaggagc tgatccgcgg
      241 ttccatcgat atggcggcga tcaccgttcc cagccagttc gatccgcggc tggagctctg
      301 caacatcaac ggcttcgtcc ggacgttcga cgaggccaga acggtcttcg ctcccgacgg
      361 atgggtattc aagaagatgg acgagttgaa ctccgcgctc ggcgtgaaac tcctcggctt
      421 ccagttcgaa gggttcatcg gcatcggaag caccaagccc gtgaacgagc cgttgaatcc
      481 cgaggtcaaa aaaggaatcc tgttgcgcat tccgaacatg gaggtcttca agctcggcgc
      541 cgaggcgatg ggctaccgga cgatcaccat cccgtgggac gaggtgcacg gctcgctcca
      601 gaacggcttt gccgagggag tgtacggaat gacgcccgcg gcggcgtaca ccatccttaa
      661 ggacgtgatc aagtactggt acgacctgcg ccactcggtg gagaatctga actacatgat
      721 gagcctgaag acctgggaga agctctccga ggaggatcgc ggaatcatcc agaacgcctg
      781 cgcgaaaatc tccgcagtca gcgtctacct tgctgagaag gatcaggaga aataccttca
      841 gagtatgcgc gacgccggta tagaagtgca cacgtatacg cccgaggagc tggcgccgct
      901 gttcgtcaag gtgtccgcga cgtgggacaa gctcgccgag cgcttctcgc aggagctgat
      961 cgacgagttc aagaaggagt tcgcccggca gtagcgtttt ttcatcggtt tttgaccgag
     1021 ttgtctgcgt acacaaacgg ggagacgccg aatattatta ggctgtctcc ccgttgtgta
     1081 ttctgaaaac tattgctttc tcgcgacgct tcccgaagtt ctcaaattcc tctctgaaaa
     1141 acgaacctgc ggatagggcc gtcacatctt gaccaagtgt accttttttg gtacattaca
     1201 ctcatgggaa tcaaagggaa aacactaacg gttttctttt ttaaatcgtc ttcagagg
//
LOCUS       DAOSWW010000915         1258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_101684_length_1258_cov_2.730673, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000915 DAOSWW010000000
VERSION     DAOSWW010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101684_length_1258_cov_2.730673"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK442_14215"
     CDS             <1..314
                     /locus_tag="PK442_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO88856.1"
                     /translation="DALFEMTSKGYGATVIVDEQGEIAGVFTDGDLRRLIGSRGVECL
                     SLEVGSVMTRNPLIIQPDKLAVEAVRVMERREISAIIVVEGKRPVGILHLHELLQAGV
                     A"
     gene            427..>1258
                     /locus_tag="PK442_14220"
     CDS             427..>1258
                     /locus_tag="PK442_14220"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOO88857.1"
                     /translation="MDERRGVYSEETRRAFDGGPLWIHAVSVGEAQSAWPLLDALRPA
                     GGEKNLFPVLLSTTTTTGKEMAFRLADSLFERHIYYPWDIPRIVNRALDAIRPRGYAT
                     METEIWPNMLFSLARRGVPAFLVNGRFSERTARTMRKHPSFWRMVYDCFTLLLVRSET
                     DKEMLLSLGVPAERVRVTGDCKVDALLLRRRNACLGPLEALFGVRDLPHDDAKRRPER
                     DPLFLAGSTHEGEEAIVLDAFMHVRRVFPSARLVIAPRHPERADGVRALASEVAPSAL
                     L"
BASE COUNT          214 a          373 c          420 g          251 t
ORIGIN      
        1 gcgacgcgct cttcgagatg acgagcaaag ggtacggcgc gacggtcatc gttgatgaac
       61 agggagagat cgcgggcgtc ttcaccgacg gcgacctgcg gcggctcatc ggttcaagag
      121 gcgtggagtg tctctcgctg gaagtgggga gcgtcatgac cagaaatccg ctgatcatcc
      181 agccggacaa gctggccgtc gaagcggtgc gcgtgatgga gcggcgggag atctccgcga
      241 tcatcgtggt ggaggggaag cgccccgtcg gcattctgca ccttcacgaa ctgcttcagg
      301 cgggggtggc ctgagctggg tacggactcg ctcacccgga gagcgggaaa agggttctac
      361 agagtctgta cctccggagc ctatcctctc ctgctcccgt ggcttctcag gaagtatacg
      421 gaggggctgg acgagcgcag aggcgtctat tcggaagaga cgcgccgtgc gttcgatgga
      481 ggcccgctct ggattcacgc agtctcggtg ggggaggccc agtccgcatg gcctctgctg
      541 gacgcgttgc gccccgccgg aggggagaag aatctcttcc ccgtcctcct ctccacgacg
      601 acgacgaccg gcaaggagat ggccttccgc ctcgcggaca gtcttttcga gcggcatatc
      661 tactatccgt gggatatccc gcgcatcgtg aaccgcgccc tcgacgcgat caggccgagg
      721 gggtacgcga ccatggagac cgagatctgg ccgaacatgc tcttttcgct cgcccgcaga
      781 ggcgtcccgg cgtttctcgt aaacggccgc ttttcggagc gcaccgcacg caccatgcgg
      841 aagcatccat ctttctggag aatggtgtat gattgtttca cgctgctcct cgtccgttcg
      901 gagaccgaca aagagatgct tctttcactc ggcgttcccg cggagagggt tcgtgtcacc
      961 ggagactgca aggtggatgc gctcttgctt cgcaggagaa atgcgtgtct cgggcctctc
     1021 gaagctcttt tcggcgttcg ggatcttccg catgatgacg cgaagaggcg cccggagcgt
     1081 gatccgctct ttctcgccgg aagcacccat gaaggcgagg aggcgatcgt gctcgacgcc
     1141 tttatgcacg ttcggcgcgt ctttccctct gcacgcctgg tgattgcgcc gagacatccg
     1201 gagcgggcgg acggcgtgcg cgcactggct tcggaggttg cgccgagcgc tttgcttt
//
LOCUS       DAOSWW010000916         1257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_101798_length_1257_cov_3.331947, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000916 DAOSWW010000000
VERSION     DAOSWW010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101798_length_1257_cov_3.331947"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PK442_14225"
     CDS             <1..858
                     /locus_tag="PK442_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOO88858.1"
                     /translation="HYSRRFDTVEVDSTFYAIPERTTVFKWAARTYPGFLFNVKAWGL
                     FTWHSVKYESLPEWVRNEVPRPESGRLDFQGLPKDARPLLWRRFTESLAPLRQMNRMG
                     YLLFQLPPKAVFSPVMMRYLERVAEVTPPFRVAVEVRNRSWMEPGNRESFLDLLKGAN
                     MAYVAVDEPELPWTVGRDAPVTASWGSVVRFHGRNTEAWSRKDATVAERFRYCYSDAE
                     MLSWEGAVRDAATKAERLFLMFNNCFRDYAVQNAQRMKCLLGVACAPGEGVQGELDFE
                     GSADEGKIE"
BASE COUNT          296 a          325 c          366 g          270 t
ORIGIN      
        1 cactattcgc ggcgcttcga taccgtcgag gtcgacagca cattctatgc gattccggag
       61 aggacgaccg tcttcaagtg ggctgcgcgc acataccccg gctttctttt caatgtaaag
      121 gcctggggtc ttttcacgtg gcactccgtg aagtacgaat cccttccgga atgggtccgg
      181 aacgaagtcc cccggcccga atcggggagg cttgatttcc agggacttcc gaaagacgct
      241 cgcccccttc tctggaggcg ttttacggag tccctcgctc ccctgcgtca gatgaaccgt
      301 atggggtatc tgctctttca acttcctccc aaggctgttt tttcccccgt gatgatgcgc
      361 tatctcgaac gcgttgccga ggtgactccg cccttccgcg tcgcggtcga ggttcggaac
      421 cgttcctgga tggaaccggg caaccgggag tcgttcctgg atttgttgaa aggggcgaac
      481 atggcgtacg tcgcggtgga cgaacctgaa ttgccctgga ccgtgggaag ggacgctccc
      541 gttaccgctt cctggggttc ggtcgtccgc ttccacggac gcaatacgga agcatggagc
      601 cgcaaagatg caaccgtcgc ggagcgattc cggtattgtt attcggatgc ggagatgctc
      661 tcctgggagg gtgccgtccg tgacgccgcg acaaaagcgg agcgcctctt cctgatgttc
      721 aacaactgct tcagagacta cgcggtgcag aatgcgcagc gaatgaaatg tctgctcggg
      781 gtcgcctgcg ctcctggaga aggagtgcag ggggagttgg attttgaagg ctccgcggat
      841 gaggggaaaa ttgaatgaaa aatgaagaaa aacagcattc ccggcttaaa ataaggatta
      901 tatccccgag actgtatact ctgtctgttt gacggatgca gcgtgcaagt gtagaatact
      961 cctaacgggt tatccggacg gagagaagaa gcatcaaaga gtttcaagtc aggaaagccg
     1021 atcaccaaag acagcaactg aaacgaggac cacgtaccaa agaaccgccg caacaaggaa
     1081 agctgtacag aacctcaaca aagatacgtg tgggatatct acagaagtac taggagctgg
     1141 ttgtttgaca gaaggtcaga accaccgtcc gagatagccc gttgaaagtc cgggagggga
     1201 agtagttccc ctcccttttc gtatgcattc aaaactgaac gcctgttaag aatgagg
//
LOCUS       DAOSWW010000917         1257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_101886_length_1257_cov_1.863561, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000917 DAOSWW010000000
VERSION     DAOSWW010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101886_length_1257_cov_1.863561"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1257)
                     /locus_tag="PK442_14230"
     CDS             complement(<1..>1257)
                     /locus_tag="PK442_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88859.1"
                     /translation="LADAFSAEKTSAPEVRPVAAEFVEDSGTASPGKAPAPEVRPAAA
                     ESAPGSGVASPGKGLEGAAGGARTAGGAQPISGAAEAAPVAATSTEIREEPLAPATPP
                     RDRTAAVPETPQKQSGAPADVVKPSETRIVQEKTPADAVFTAKAPADVVSIEKASVDA
                     LSAEKAPADVVSAEKAPADVVSAEKVYARRSDSAPFFSELSRGGGDESAALSAPRFSL
                     VMTDNVRPRRMLMYERTPAEIGAEASIRFRSAVAALRNGVQEVRSTNVREPLMSSVPR
                     DFQAAVKAEQILRSAVESELSTNALVQEDQPGHSLFSADRALFAGNEKNAHNIRSEEL
                     QLRSAPSGRELPSGDSEKTMVHTPGAAESVAVSSSQGMRSNFQDLFSREVVLQARGGA
                     ALEDGLQHVVRFLRAEGRPAASIIIDP"
BASE COUNT          223 a          408 c          380 g          246 t
ORIGIN      
        1 ggatcgatga taatgctggc ggcgggcctt ccctccgcgc ggagaaaacg tacgacatgt
       61 tgaagaccgt cctcaagcgc cgcgccgcct cttgcctgca atacgacctc tctcgaaaac
      121 agatcctgga agttgcttcg cattccctga gaggatgaaa cagctacgga ttccgccgcg
      181 cccggcgtat gcaccatggt tttttcagag tctccggacg ggagctctct cccggatgga
      241 gccgaccgga gctgaagctc ttcactccgg atgttgtgcg catttttctc gttccccgcg
      301 aagagcgcgc ggtcagctga gaagaggctg tgaccgggct ggtcttcctg cactaaagca
      361 tttgtagaaa gctccgattc cacggcggag cgcaggatct gttccgcttt gacggcggcc
      421 tgaaaatccc ggggaaccga ggacatcaaa ggttcgcgca cgtttgtgga gcgaacttcc
      481 tgtacgccgt ttcgaagagc cgccacagca ctccggaagc gaattgaagc ctccgcgccg
      541 atctccgccg gcgtgcgttc atacatgagc attcgccggg gacggacgtt gtcggtcata
      601 acgagcgaga atcgcggggc ggaaagtgcc gccgactcat ctccccctcc ccgggagagt
      661 tctgaaaaga acggcgcaga atctgaccgt cgggcataaa ccttctccgc agatacgaca
      721 tccgcgggag ccttctccgc agatacgaca tccgcgggag ccttctccgc ggatagggcg
      781 tccacggaag ctttctcaat ggatacgaca tctgcgggag ctttcgccgt aaacactgcg
      841 tccgcgggcg ttttctcctg gacgatccgc gtctccgagg gctttacaac gtcagcgggc
      901 gctccgctct gcttctgcgg cgtttcgggc actgcggccg tccggtcacg cggcggcgtt
      961 gcgggggcaa ggggttcctc acggatctcg gtacttgtcg ctgctacggg cgctgcttcc
     1021 gctgcacccg aaatcggttg tgctcctccc gcagtacgcg ctcctccggc cgctccttca
     1081 agccccttcc cgggcgaggc gactcccgaa cccggtgcag actccgcagc tgcggggcga
     1141 acctccggcg cgggagcctt cccgggcgat gccgttcccg aatcctccac aaactccgcg
     1201 gctacggggc gaacctccgg cgcggatgtc ttctccgcag agaatgcgtc tgcgaga
//
LOCUS       DAOSWW010000918         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_102265_length_1254_cov_2.494579, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000918 DAOSWW010000000
VERSION     DAOSWW010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102265_length_1254_cov_2.494579"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PK442_14235"
     CDS             <1..1073
                     /locus_tag="PK442_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOO88860.1"
                     /translation="VSGNSGGGGIHNGRTMTLNNCVIKDNSSSGSIKAGGISSTDYGY
                     DATTLTMRNCVVENNTCTDGIAGGIYNGVKGTLTMYRCIVRRNSLSSMGGGLYISSST
                     TTHLADYCQVTENTPDQICCAYGVTYTTDGTCTIGDTAGKSAVALSGAAEGSSVKPRN
                     TTGEADVTKVENDLKNRESAIFREAENLLSTDLGGLPGGAAATLYDAFAYENVPLADT
                     SGKGELEVQFTASWPMNARYYAALAEYEDAAAGVQAVKEYVIPERSVHFEIKPGQPLP
                     DGVTPPDFYEEGEGLMTWRNVVEDNGPFDHNPTVGVVTFRVASIRAEAQSASSGGSGC
                     SAVGASPLALLMALPLLFLRKQ"
BASE COUNT          277 a          359 c          396 g          222 t
ORIGIN      
        1 ccgtgagcgg gaactccggc ggaggcggca ttcacaacgg aaggaccatg actctgaaca
       61 actgcgtcat caaggacaac tcgtcgtccg gaagcataaa ggcgggagga atcagcagca
      121 ccgactacgg atacgacgcg acgacgctga cgatgagaaa ctgcgtcgtc gagaacaaca
      181 cgtgtaccga tggaatcgcg gggggcatct acaacggcgt caaaggaacg ctcaccatgt
      241 accggtgtat cgtgaggagg aacagtctga gcagcatggg aggggggctc tatatttcgt
      301 cctccacaac gacgcatctc gccgactact gccaagtgac ggagaacact cccgaccaga
      361 tatgctgcgc ctacggcgtc acctacacca ccgacggcac ctgtaccatc ggcgacacgg
      421 ccgggaaatc cgccgtggcg ctttcgggag ccgcggaggg gagttctgta aaacctcgga
      481 atacgacggg tgaggcagac gtgacgaagg tggagaacga tctgaagaac cgtgagagcg
      541 cgatcttcag ggaagcggag aacctcctct cgacggatct tggaggcctt cccggaggcg
      601 ccgccgcgac gctctacgac gctttcgcct acgagaacgt cccgctcgct gatacgtccg
      661 gaaaagggga gctggaagtg cagttcaccg cctcgtggcc gatgaacgcg cgctactacg
      721 ccgcgcttgc ggagtacgag gacgcggcgg cgggcgtgca ggctgtgaag gagtacgtta
      781 tccccgagcg gagcgtgcac ttcgagatca agccggggca gccgcttccc gacggcgtaa
      841 cgcctcccga cttctacgag gagggcgagg ggctgatgac ctggcgcaac gtcgtggagg
      901 acaacggacc gttcgaccac aacccgacgg tcggcgtggt caccttccgc gtggcgtcga
      961 tccgcgccga ggcgcagtcg gcctcctcgg gaggatcggg ctgctcggct gtcggcgcgt
     1021 cgcccttggc gcttcttatg gcgctcccgc tgctgttcct gcggaaacag tagggctttc
     1081 ttccatcaga acgaaaaaac aggggagggc ggagaagttc atttcttcgc acctccctgt
     1141 tattttcagc aaagattgaa tgatattggt cccgacagat gattgacatt tcggcagcaa
     1201 agcttaaaaa aagaacggga ccgggttcag ttcttcctct ttcaacgttt tttt
//
LOCUS       DAOSWW010000919         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_102368_length_1253_cov_3.492487, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000919 DAOSWW010000000
VERSION     DAOSWW010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102368_length_1253_cov_3.492487"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1253)
                     /locus_tag="PK442_14240"
     CDS             complement(<1..>1253)
                     /locus_tag="PK442_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88861.1"
                     /translation="RPFRSLSFPLPLVQWTGEAPRLVFLSEQGALFGEYDLDEEGISV
                     RKLHSPSCLQILDQRDGKYLLSLRGERLAEQGYSGDSYTYLLTTQSGKVTVLVKNSRH
                     FSGWFAPDGGIVLGDWGRPAGIRRYSPLGVPEGEFVLEECRTRYGFFVSQSEMLFPSD
                     FFPYTFIKVDTLTGVCTGMYPFPPGMTMHFADSFFRDGKVYSFSSSRPSISSPLRGAN
                     LICFDASGQGWLDIAMAPLDPHAGAPMSTFEQTEVAVRFLHEYGRSFGKQLDVTFGGG
                     TVRSADPEGGRYVWETPPTGGAEFVVVPVEVRLGPVSRVFEVTLRRLHNPLQLAVTKR
                     IQTDRNGYIKLFADWELTNPAPYDLNDLAWSFSGTNLKLSGLSGLPRSMRAGETRSGS
                     VEVDMLYEGMESEPSVWGYSVEGRG"
BASE COUNT          256 a          395 c          385 g          217 t
ORIGIN      
        1 cccccgcccc tcgacggagt acccccacac cgagggctcg ctctccatcc cttcgtagag
       61 catatccacc tctacggagc cgctgcgcgt ctccccggcg cgcatgctcc gcggaaggcc
      121 ggaaaggccg gagagcttca ggttcgtccc cgaaaaggac caggcaagat cgttcaggtc
      181 gtacggcgcg gggttggtca gctcccagtc ggcgaagagt ttgatatacc cgttccggtc
      241 cgtctgtatc cgcttcgtta cggccagctg cagcggattg tgaaggcgac gcagcgtcac
      301 ttcgaagact cttgacaccg ggccgagacg aacttccaca ggaaccacga cgaattcggc
      361 gcctcctgtc ggaggcgttt cccagacgta tcgcccgcct tcgggatccg cgctgcgcac
      421 cgttccgcct ccgaacgtca cgtcgagctg ttttccgaag ctgcgtccgt actcgtgcag
      481 gaagcgcacc gcgacctcgg tttgctcgaa ggtggacatc ggagcacccg cgtgcgggtc
      541 gagcggcgcc atcgcgatgt cgagccaccc ctgaccggag gcatcgaagc agatcaggtt
      601 ggcgcctcgc agcggagacg atatcgacgg gcgcgacgag gagaaggagt agacctttcc
      661 gtcgcggaaa aaactgtccg cgaagtgcat ggtcatcccc ggagggaagg ggtacatgcc
      721 ggtacagacg ccggtgaggg tatccacctt gatgaaggtg taggggaaaa agtcggaagg
      781 gaagagcatt tcgctctgcg agacgaagaa gccgtacctt gttctgcact cttcaagcac
      841 gaactccccc tccggcacgc cgagcgggga gtacctccgg atacctgcgg gcctccccca
      901 atctccgagg acgattcccc cgtcaggcgc gaaccagccg gaaaagtgcc tgctgttttt
      961 cacgagcacc gtaaccttgc cggactgcgt cgtcagcaga tacgtgtagg agtccccgga
     1021 gtacccctgc tccgccagcc gttcgccgcg aagggagagg agatacttcc cgtccctttg
     1081 gtccagtatt tggagacatg aaggagaatg gagcttgcgg acggagatcc cctcctcgtc
     1141 caggtcgtac tcaccgaaga gggcgccttg ttccgagaga aaaaccagac gcggcgcctc
     1201 gcctgtccat tgcacaagcg gaagaggaaa cgaaagcgaa cggaagggcc ttt
//
LOCUS       DAOSWW010000920         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_102382_length_1253_cov_3.118531, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000920 DAOSWW010000000
VERSION     DAOSWW010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102382_length_1253_cov_3.118531"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PK442_14245"
     CDS             complement(<1..265)
                     /locus_tag="PK442_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88862.1"
                     /translation="MKLCREHGVRYAERTSPEGSLHHAECVTRFSRDWLGEQPLLYAQ
                     AGGEITVGRTYEELFAGGVVKKWRVDALIDFLATGTCVFPGGNT"
     gene            complement(367..711)
                     /locus_tag="PK442_14250"
     CDS             complement(367..711)
                     /locus_tag="PK442_14250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HOO88863.1"
                     /translation="MSAESAVAFYERLEKDIELQEKLKELGTKEKIEQHVKGELGYDF
                     TLEEMQKVIFEKNPDLTDDELEAVVGGNINVGVVVGGLVGIIGLTGIGVAAGAFAGFT
                     LFGVVAAGVAAA"
     gene            complement(788..1096)
                     /locus_tag="PK442_14255"
     CDS             complement(788..1096)
                     /locus_tag="PK442_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HOO88864.1"
                     /translation="MSIESAAAFYDRLQNDDALQEQLKAAGDRDRVEAYVRDKLGYDF
                     TKEEMQKVIFERNPEMSDEELEAVVGGVSDDMLWGITIGVGVGGGMGIIALAAAAAAA
                     "
BASE COUNT          239 a          443 c          277 g          294 t
ORIGIN      
        1 aggtattgcc gcccggaaag acgcacgttc ccgtcgcaag gaagtcgatc agggcgtcaa
       61 cccgccactt cttcacgacg cctccggcga aaagctcttc gtaggttctt cccacggtga
      121 tttccccgcc cgcttgagca tagagcaggg gctgctctcc cagccagtcg cgcgaaaaac
      181 gcgtaacaca ttcggcgtgg tgaagacttc cttccggaga ggtgcgctct gcatacctga
      241 caccatgctc tctacacaac ttcaacacat ccttctccgt cagctctgaa acgggatcat
      301 gtaggattct tagcctttgc ggcacaggtc ctttagcttt tagaacatcc agctctccgc
      361 cgcgtattac gctgctgcga ctcctgcggc cacaacgccg aagagagtga acccggcgaa
      421 cgcccctgcg gcgacgccga tgcctgtcag tccgataatt ccgacgagtc cgccaacgac
      481 gacgccaaca ttgatattgc ctcccacgac cgcctccagc tcgtcgtcgg tcaaatccgg
      541 attcttctcg aagatcacct tctgcatctc ctccagcgtg aagtcgtacc cgagctcccc
      601 cttcacgtgc tgttcgatct tctcctttgt cccaagctcc ttcagtttct cctgcaactc
      661 gatatccttc tccaaacgct cgtagaacgc taccgcggat tccgccgaca ttcttgactc
      721 ctccctcgaa tgcgctcctc ccttgcgaaa ccagacggtt ccgcaacggc ggctgcgctc
      781 ttttccgcta cgctgccgca gcggcggccg ccagcgcgat aatccccatc cccccgccga
      841 cgccgacgcc gatagtaatc ccccacagca tatcgtcgct tactcctccg acgacggcct
      901 ccagctcttc gtcgctcatc tccgggttcc gttcgaaaat caccttctgc atctcttcct
      961 ttgtgaagtc atacccgagt ttgtcccgca cgtacgcttc cacccgatcc ctgtcgcccg
     1021 ccgctttcag ttgttcctga agcgcgtcgt cgttctggag acggtcgtaa aacgcagcag
     1081 cagattcaat cgacatggct cttcctcctt tttcgtacag tccggaaacg aaaagagggg
     1141 gaaatgcgtc gttccccctc tttttcagtc attgcatcgt tttcaacccg gtatcaaacg
     1201 catgcaacct tctccgcgtt cttatctcaa ttgtcgcccc agcgctggaa cca
//
LOCUS       DAOSWW010000921         1253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_102411_length_1253_cov_2.555092, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000921 DAOSWW010000000
VERSION     DAOSWW010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102411_length_1253_cov_2.555092"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1185)
                     /gene="truD"
                     /locus_tag="PK442_14260"
     CDS             complement(1..1185)
                     /gene="truD"
                     /locus_tag="PK442_14260"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOO88865.1"
                     /translation="MRIKTAPDDFRVEEVLHFAPAKKEAPFRVFLLEKEGWNTADAIQ
                     EAAKACGVPLAEIRSVGKKDRHAHTLQHVSVPLKYDLAYEGKGFVIRFAGYSTAHLAP
                     SVLKGNRFSAALRDMTAAEAGACEARLERIAARGFANYFDDQRFGNVGEKGEFFAEML
                     VKRRFGPALRKYFSFRHPEAPLPLKKRKAQLAALWGKWEEMVPLCPEPLLKQMLDILR
                     RKGEGGYRACLKLIPAQEMGLYYSAYQSFLWNETLCRFLSKQPGELFPVQVKTGELLQ
                     YRMLDESLLSRLLTEPMHTAAPDMPQLPSDLGDVFSAVLFERGVRLSKFRLKEVEQAY
                     FSSFPRACAVIPENMTWKREEDALFPGKTALRVDFFLPRGSYATMMLKSAEEGGFQEP
                     RT"
BASE COUNT          238 a          401 c          375 g          239 t
ORIGIN      
        1 ctacgtccgc ggctcctgaa atcctccctc ttcggcgctc ttgagcatca tcgtcgcata
       61 acttcctcgc gggaggaaga agtcgacgcg aagcgcggtt tttcccggga agagggcatc
      121 ctcttccctt ttccatgtca tgttctccgg aatcacggcg caggcgcgcg ggaaggagga
      181 gaagtacgcc tgctcgacct ctttcagccg gaatttcgag agacggactc cccgttcgaa
      241 aagcaccgcg gagaaaacat cgccgaggtc ggagggaagc tgcggcatat ccggcgccgc
      301 agtgtgcatc ggctcggtaa gcaggcgcga aagaagcgat tcatcaagca tgcggtactg
      361 aaggagttcg ccggttttca cctgcacagg aaagagctcg cccggctgct tcgaaaggaa
      421 gcggcagagc gtctcgttcc acaaaaagct ctgataggcc gagtagtaga gccccatctc
      481 ctgcgcgggt atgagcttga gacacgctct gtacccgccc tcccccttgc ggcggaggat
      541 gtcgagcatc tgtttcaaca gaggttcggg acagagcggg accatctcct cccactttcc
      601 ccagagagcg gcaagctgcg cctttctctt tttaagcggc aggggcgctt cagggtgccg
      661 gaaggagaag tacttcctga gggcgggtcc gaagcgtcgt tttaccagca tctcggcgaa
      721 aaactctccc ttctctccca cgttgccgaa gcgttgatcg tcgaagtagt tggcgaagcc
      781 gcgcgcggcg atccgctcca gccgcgcctc gcacgccccg gcctcggcgg ctgtcatgtc
      841 gcggagcgca gcggagaagc ggttgccttt cagcacggac ggcgccaggt gcgccgtgga
      901 gtaccccgca aagcgaatga cgaacccctt tccctcatac gcaagatcgt acttcaacgg
      961 cacggaaacg tgctggagcg tgtgcgcgtg ccggtctttc ttccctaccg agcggatctc
     1021 ggcgagcggg acgccgcagg ccttcgccgc ttcctgaatt gcatcggcgg tgttccaccc
     1081 ctccttctcc agcaggaaga cgcggaacgg cgcctccttc ttcgccggag caaagtgaag
     1141 tacctcttcc acacgaaaat catcgggagc cgtcttgatt cgcatctgtc cttctcctca
     1201 gcaggcgggc gctaggccag cgcaccgcgt gccgcgacga cgccgctcgc cgc
//
LOCUS       DAOSWW010000922         1247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_103184_length_1247_cov_4.368289, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000922 DAOSWW010000000
VERSION     DAOSWW010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103184_length_1247_cov_4.368289"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PK442_14265"
     CDS             <1..1151
                     /locus_tag="PK442_14265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88866.1"
                     /translation="ITERKKLIESLVLAKEKAEESDRLKTAFLQNVSHEIRTPMNGIL
                     GFLHLLGQPALDDDERSEYIRIVNQSGQRLLSTINDIIEASRIEAGKTTVQMDDVDVE
                     QTMAFHFAFFRPRAEEKGLFLTLSEHLRGEEALLRTDKHKLDSILNVLLGNALKFTCS
                     GGVEFGNRLEGSELLFFVKDSGEGIPEDRLEAVFDSFVQADLGVARSHEGAGLGLTIA
                     KANVEALGGKIDVRSEVGRGSTFTFSIPYLPLTPGAVISKSGAGAHSAEADQGGSLIL
                     IAEDDSTSFRLLEAMLKKAGYSVLRAVDGEEALRAVRENPDVALVLMDVKMPGMDGLE
                     ATRRIRRFNTVLPIIAQTAYAFPEDRTKALEAGCDDYISKPVERTALL"
BASE COUNT          290 a          324 c          391 g          242 t
ORIGIN      
        1 atatcactga gagaaagaag ctgatcgagt cgcttgtgct cgccaaggaa aaggccgaag
       61 agagcgaccg gctgaagacg gcgttcctcc agaacgtatc gcacgagata cggacgccga
      121 tgaacggaat tctcgggttt cttcacctgc tcgggcagcc ggctttggac gacgacgagc
      181 gaagcgagta catccggatc gtcaaccaga gcggacagag gctcctgagc acgatcaacg
      241 atattataga agcgtccaga atcgaagcgg gaaagacgac ggtgcagatg gacgatgtcg
      301 acgttgaaca gacgatggcg ttccatttcg ctttcttccg gccccgggcg gaggagaagg
      361 ggctcttttt gacgctttcg gagcatcttc ggggagaaga ggcgttgctc cgcaccgaca
      421 aacacaagct ggacagcatc ttgaacgttc tgttgggcaa cgcgctcaaa ttcacctgca
      481 gcggaggcgt cgaattcggg aatcgactgg aaggctccga actgctcttt ttcgtgaagg
      541 acagcggaga gggaatcccc gaggatcgtc tcgaagccgt cttcgacagc tttgtgcagg
      601 cggatctggg ggtggcgcgt tcgcatgaag gcgccggatt gggactgacc atagcaaagg
      661 ccaacgtcga agccctcggc gggaaaatcg atgtacgttc cgaggtcggc agagggagca
      721 cgttcacttt ttcgattccg tatctgccgc tcacgccggg cgccgtgatt tcaaagagcg
      781 gcgctggagc ccattccgca gaggcggatc agggcggttc tcttatcctg attgccgagg
      841 acgactcgac cagtttccgt cttttggagg cgatgctgaa gaaggcgggg tattcagtgc
      901 ttcgcgccgt cgacggggaa gaagcgctgc gggctgtacg cgaaaacccg gacgtagctc
      961 ttgttctgat ggatgtaaaa atgccgggaa tggacgggct ggaggcaaca agacggatac
     1021 ggcgcttcaa taccgtgctc ccgatcatcg cgcagacggc gtacgccttt ccggaagacc
     1081 gcacaaaagc attggaagca gggtgcgacg actatatctc caaacctgtc gaacggacgg
     1141 cgctgctgta gatgatcaag cgctactgcg aaaggaaaga cgcttgagcg ccccccctct
     1201 ctttgaaatg aagggaaatt gcccctcttc cgtgaaagca gtctttc
//
LOCUS       DAOSWW010000923         1247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_103291_length_1247_cov_1.865772, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000923 DAOSWW010000000
VERSION     DAOSWW010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103291_length_1247_cov_1.865772"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK442_14270"
     CDS             complement(<1..204)
                     /locus_tag="PK442_14270"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOO88867.1"
                     /translation="MSESTPDIAAELLRFRPLVLATARRYSGRGAEFDDLVQEGYMAL
                     LELIPRCPRRDLLPLFLISRLPGR"
     gene            complement(204..560)
                     /locus_tag="PK442_14275"
     CDS             complement(204..560)
                     /locus_tag="PK442_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HOO88868.1"
                     /translation="MTTQSKRLNDMRISEHFRLREFECPCCRCVMIAPGLVCRLEALR
                     ELWGKPIMITSGYRCPEHNVWVKGSPKSLHMRGQAADVVVPFEEQEAVMAMARKADFS
                     SVIPYGKRNFIHLAVN"
     gene            complement(625..819)
                     /locus_tag="PK442_14280"
     CDS             complement(625..819)
                     /locus_tag="PK442_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HOO88869.1"
                     /translation="MDELVATMMQSGFSVAVAAFLLVRMERRLEDLTLAIRELHGAIL
                     RGNVAGGANAGGTDGLPVAR"
     gene            complement(885..1085)
                     /locus_tag="PK442_14285"
     CDS             complement(885..1085)
                     /locus_tag="PK442_14285"
                     /inference="COORDINATES: protein motif:HMM:NF022597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HOO88870.1"
                     /translation="MKTLRMKFATDEGKTFTISLNYAREDLTAGEVETAMEAMLDEEL
                     FAEGLAGLSGADLVDRTVTPLF"
BASE COUNT          222 a          472 c          333 g          220 t
ORIGIN      
        1 gcgccccgga aggcggctta tcaagaagag ggggagcaga tcgcgccttg gacagcgtgg
       61 aataagctcc agcaacgcca tatacccctc ctgcaccaga tcgtcgaact ccgcccctcg
      121 cccgctgtaa cgcctcgccg tggccagcac cagcggccgg aagcggagca gctccgcggc
      181 gatatccggc gtgctctcgc tcattagttc accgccagat gtatgaaatt gcgcttcccg
      241 tacggaatca ccgacgagaa gtccgctttc cgcgccatcg ccatcacggc ctcctgctcc
      301 tcgaagggca cgaccacgtc cgccgcctgc cccctcatgt gcaggctctt cggcgacccc
      361 ttcacccaga cgttgtgctc cgggcatcgg tagccgctgg tgatcatgat cggcttcccc
      421 cacagctccc gcagcgcctc cagccggcac accagccccg gcgcgatcat aacgcagcga
      481 cagcacggac actcgaactc ccgcagacgg aagtgctccg aaattcgcat atcgttcagc
      541 cgcttggatt gtgtcgtcat tctgcttccc tctcctgtaa aaatgtatat tctcggcgtg
      601 ttccgcgccg tgttcccgca gccgtcagcg cgccacggga agcccgtcgg ttccgcccgc
      661 gttcgcgccc cccgcgacgt tcccccgcag aatggcgccg tgcagctccc tgatggccag
      721 cgtcagatcc tccagccgcc gctccatccg caccagcaaa aacgccgcca cggccacgga
      781 gaagccgctc tgcatcatcg tcgctaccag ctcgtccata cgcgcgctcc ctccttcagc
      841 tgctcaaatg gggggaggcg aacctccccc cggtcgaccg gccgctagaa cagcggcgtc
      901 acagtgcggt cgacaagatc ggccccggaa agcccggcaa gcccctcggc gaacagctct
      961 tcgtcgagca tcgcctccat cgccgtctcc acctcgcccg cggtcaggtc ctcgcgagcg
     1021 tagttcagcg agatggtgaa cgtctttcct tcatcggtcg caaacttcat acgaagcgtc
     1081 ttcattcggc atcacccttt cgttcgccgg ccgcttacgc ggactccagc agatcggttc
     1141 cggccttctc caccgcgatc acagggtgga tgaggagcgg cgtgaccaga tcgcccaccg
     1201 ccttcagatt gtccgccaca agatcgggat ccaccccgcg gatcgac
//
LOCUS       DAOSWW010000924         1247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_103293_length_1247_cov_1.849832, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000924 DAOSWW010000000
VERSION     DAOSWW010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103293_length_1247_cov_1.849832"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1247
                     /locus_tag="PK442_14290"
     CDS             <1..>1247
                     /locus_tag="PK442_14290"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOO88871.1"
                     /translation="ASMDAVQLSNVFQLASNLEDGGFFGREFVDEVLEMLAREKEFAE
                     SVLPAEVVRLMLEVADIRPGERIYTPFDNCLQLSIAVEEQSGGNAPVFAEFPEASALP
                     WLLRLFYGSASAIKEGDPVLAPSFFSGERTLDRFDTALAVLPFSRKYARFDAGRDPLD
                     RFPEATASAGVLALRHILAQTKKRAVAAVANSLLFSSGAERALREHLVDTGKVEAVIG
                     LPSALLAGSSVAFSLLVLKLDAPSETIRFIDGATEDAEGLFFTKDGKGRATLSGWQRM
                     ADLYRSGATDRHANQAARSEVRPPEYRLEVSRYCLSEQERAVETYLFSRDTKVLSEIA
                     EIIRPVPPLRQDAEEQGAEALELEVSSLPRYGPAKPAGKLVFLREKDLENDKGRSFLR
                     TGDVVLAVKGRTGRVGIIGAGVS"
BASE COUNT          241 a          379 c          396 g          231 t
ORIGIN      
        1 gtgcgagcat ggatgcggtc cagctttcga acgtcttcca gcttgcgtcc aaccttgaag
       61 acggcgggtt tttcggcagg gagttcgtcg atgaagtact cgaaatgctc gcccgcgaga
      121 aggagtttgc ggagtccgtc cttccggcgg aagtggtccg gctgatgctc gaagttgcgg
      181 atatacgccc cggagaacgc atctacacgc cttttgacaa ctgcctgcaa ctgagcattg
      241 cggtcgagga acagagcggg gggaacgctc cggtcttcgc cgagtttccg gaagcgtcgg
      301 cgctgccctg gctgctgcgc ctcttctacg gaagcgcgtc cgcgatcaag gagggggacc
      361 ccgtcctcgc gccttctttt ttttccggag aaaggacgct tgaccggttc gacaccgctc
      421 ttgccgttct gccgttctcc aggaaatacg cccgtttcga cgccgggaga gacccgttgg
      481 atcgcttccc ggaagcgact gcctcggcag gcgtgctcgc cctcagacac attcttgccc
      541 agacaaaaaa gcgggccgtc gcggctgtcg ccaacagcct gctcttcagt tcgggagcgg
      601 agcgcgcgct gcgggagcat cttgtggata cagggaaggt agaagctgtt atcggcctgc
      661 cctccgccct gctcgccgga tcgtcggtcg cattctcgct tctggtattg aagctggacg
      721 ccccttcgga aacaatacgc ttcatcgacg gagcgaccga agacgccgaa gggcttttct
      781 tcaccaaaga cggcaagggg cgcgcaacgc tctccggatg gcagcgcatg gcggacctgt
      841 accggagcgg cgcaacggac aggcacgcga atcaggcggc ccgctccgaa gtgcgcccgc
      901 ccgagtaccg cctggaagta tcccggtact gcctctcgga gcaggaacgg gcggtcgaaa
      961 cctatctttt cagccgggac acaaaagtgc tgtccgaaat agcggagatc atacgccccg
     1021 ttcctccgct ccggcaggat gccgaggaac aaggcgccga agcgcttgag ctggaagtgt
     1081 cgtccctgcc caggtacggc cctgcgaagc cggccggcaa actggtgttt ctaagagaga
     1141 aggacctcga aaacgacaag gggagatcgt tcctccggac cggcgacgtc gtccttgcgg
     1201 tcaagggcag aaccggaaga gtgggcatca taggagccgg cgtatcc
//
LOCUS       DAOSWW010000925         1244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_103710_length_1244_cov_1.936081, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000925 DAOSWW010000000
VERSION     DAOSWW010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103710_length_1244_cov_1.936081"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /gene="dctP"
                     /locus_tag="PK442_14295"
     CDS             complement(<1..623)
                     /gene="dctP"
                     /locus_tag="PK442_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO88872.1"
                     /translation="MTFTSTTKRILVCLVTLSFLFAFFGMASAADPLFTMRLAQVHPV
                     DSDYDLRAKSFAQKVFDRTEGRIKIDVFSGGVLGDWTEIFEMVQRGTIEMSMAQANAN
                     FDPQLNLAYYFPYIVTNASEAQKVYGPVGWAYKIIQDLWAKHNIMALSVVPIGMAGVS
                     LNKVPEKYTEPGASHGLKVRVMPIKPCEWTYEALGYIATPTPYAEAY"
     gene            complement(740..>1244)
                     /locus_tag="PK442_14300"
     CDS             complement(740..>1244)
                     /locus_tag="PK442_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOO88873.1"
                     /translation="IMFRSLKSAATTSGVIVLMLFFVTILGRLYTMQQVPMKIANFLL
                     STSDNKYVILFMVNIFLVIIGMLMDDLSGTMLAAPLLMPLMLRIGVHPIHFAAILGTN
                     LGLGNVTPPTAPILYLGGRVGGVQVDEYMKPALIYMIFGFIPVIILTTYLPDLSLFLP
                     RLIMGIK"
BASE COUNT          256 a          332 c          391 g          265 t
ORIGIN      
        1 ctgtaggcct ctgcgtaggg agtcggcgtt gcgatgtagc cgagggcctc gtaggtccac
       61 tcgcacggct tgatcggcat gacgcgaacc ttcaggccat gggatgcgcc gggttcggtg
      121 tacttctcgg gaaccttgtt cagcgagacg ccggccatac cgatgggaac cacggagagg
      181 gccatgatgt tgtgcttcgc ccacagatcc tggatgatct tgtacgccca tcctacggga
      241 ccgtagacct tctgcgcttc cgaggcgttc gtcacgatgt aggggaagta gtaggcaagg
      301 ttcagctgcg ggtcgaagtt cgcattggcc tgcgccatgc tcatctcgat cgtgccgcgc
      361 tggaccatct cgaaaatctc ggtccagtcg ccgagaacgc cgccggagaa aacgtcgatc
      421 ttgattctgc cttccgtacg gtcgaaaacc ttctgtgcga aggacttggc cctcagatca
      481 tagtcgctgt cgacggggtg tacctgcgcc aggcgcatcg tgaacagagg atcggcggcc
      541 gatgccattc cgaagaaggc aaagaggaac gaaagcgtga cgaggcaaac aagaatccgc
      601 ttcgttgtac tggtgaacgt catagtatcc ctccttttga atatgtatcc ttatccgata
      661 agacgcttgt ccgtgcgcgt ttccttacgt ttcgggcccc ggcatcgcct cctctcgtag
      721 tacgggatgt ctttgcttgt tacttgattc ccatgatcag ccggggaagg aagagcgaga
      781 gatccggcag atacgtggtc aggatgatca cgggaatgaa cccgaagatc atgtagatca
      841 gcgcgggctt catgtattcg tccacttgca cccctccgac gcggcctccg aggtagagga
      901 tcggcgcggt cggcggcgtt acgttgccga gcccgaggtt cgtccccagg atggcggcga
      961 agtggatcgg atgcacgccg atgcggagca tcaggggcat cagaagcggg gcggcgagca
     1021 tcgtgccgct gaggtcgtcc atcagcattc cgatgatgac aaggaagatg ttgaccatga
     1081 agagaatgac gtacttgttg tccgacgtgc tcagcaggaa gttcgctatt ttcatcggca
     1141 cctgctgcat ggtgtagagc cgtccgagaa tggtgacgaa gaagagcatc aggacgatga
     1201 cgccgctcgt tgtcgccgcg gacttgagcg agcggaacat gatg
//
LOCUS       DAOSWW010000926         1242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_103938_length_1242_cov_2.946083, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000926 DAOSWW010000000
VERSION     DAOSWW010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103938_length_1242_cov_2.946083"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..>1242
                     /locus_tag="PK442_14305"
     CDS             171..>1242
                     /locus_tag="PK442_14305"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOO88874.1"
                     /translation="MKKYRILASFLILALCIVAAPASAKYMIVGNDEKMTWNEKGAVV
                     NLPSGKDTVQIFDISEGGTEPKLLATLELMNSISGPPTNLAITKDEKLAILANPLEWA
                     EKDGKWSGSPSTKIYVIDLEGDAPKHIATVDGGRQPSGLAINRAGDLMLVANRADNSV
                     GVFSIEGKTVTLIDTVPMDEQVAGVAITPDGTKAWVVKNLSHKLALLNIDGKKVTPAK
                     FEIPVLWPYNVDITPDGSLALGADMGGPGGSSGHVDTVSVIDMSQNPPRVIDRVVAGD
                     GPEGFAVSPTGEYLATVNIRGSNVAHDSWFYHKNGTVSLLKIDGKKLTKVNEVEVGAL
                     PEGVVWSPDGKWLYVGNFLDKNI"
BASE COUNT          272 a          362 c          379 g          229 t
ORIGIN      
        1 attttttcgg cgggaaacgt ttggggttct ctaatgatga ccgaaagcgt ttcgtagccc
       61 ccggaggtga tagagaaccg gtaataagtg aatgcatgcg caaatcggtg aggtcgggac
      121 atcacgtgta tcggcgtagt gttggttgag tatagaagga gcgtgcctca atgaaaaagt
      181 accggattct tgcgtctttc ttgatcctcg cgctgtgcat cgtcgcagcg ccggcttcgg
      241 cgaagtacat gatcgtgggc aacgacgaga agatgacgtg gaacgaaaaa ggagccgtcg
      301 tgaatctgcc ttccggaaag gatacagtgc agatcttcga catctccgaa gggggaaccg
      361 agccgaagct tctggccact cttgaactga tgaactccat ctccggacct cccacgaacc
      421 tggcgattac gaaggacgag aagctcgcca tcctcgcgaa ccctctggaa tgggctgaga
      481 aggacggaaa gtggagcggc tccccgtcca cgaagatcta cgtcatcgac cttgaaggcg
      541 acgcgccgaa acacatcgcc acggttgacg gcggacggca gccgtcgggc ctcgcgatca
      601 acagggccgg cgacctgatg cttgtcgcca accgggcgga taactccgtc ggcgtcttct
      661 ccatcgaggg gaagaccgtg accctcatcg acaccgtccc gatggacgag caggtcgccg
      721 gggtcgccat cactccggac ggcacgaagg cctgggtcgt caagaacctt tcgcataagc
      781 tcgcgctgct caacatcgac ggaaagaaag taaccccggc gaagttcgag atccccgtgc
      841 tctggccgta caacgtggac atcacgccgg acggctccct ggccctgggc gccgacatgg
      901 gcggccccgg cggctcttcc ggccatgtgg acaccgtctc cgtcatcgac atgagccaga
      961 atccgccccg cgtgatcgac cgtgtggtcg cgggcgacgg ccccgaaggg ttcgccgtct
     1021 ctcccacggg agagtacctt gcgaccgtga acatccgggg aagcaatgtt gctcacgact
     1081 cctggttcta tcacaagaac ggtaccgtct ctctgctcaa gatcgacgga aagaagctca
     1141 cgaaggtgaa cgaggtcgag gtcggcgcgc tcccagaggg cgtcgtctgg agccccgacg
     1201 gcaagtggtt gtatgtcggc aacttcctcg ataagaacat ct
//
LOCUS       DAOSWW010000927         1237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_104666_length_1237_cov_3.326565, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000927 DAOSWW010000000
VERSION     DAOSWW010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104666_length_1237_cov_3.326565"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PK442_14310"
     CDS             complement(<1..1140)
                     /locus_tag="PK442_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOO88875.1"
                     /translation="MKLSVLKGFRPGPIEADFLNMLMFDIGGGMRSVSLPRGYVSEAI
                     FRDGIGFDASNYGFAKVDQSDMVAIPDCNAAWLEERGDFSIVHVLCDVVSTERKVFDQ
                     YPRSVAERTAEYLKEKGLADSAQMLVELEYYVFDSVEYSTGLDHAFYSIGSGEGLGED
                     FNTQQRFAPLKGYHKLPPEDRFFDFRNETVRLMEEAGIAVKYHHHEVGASQLEIELDF
                     MDLVKAADSVCIAKWIIHSVAEEMGFKVTFMPKPLYKMPGSGMHVHQFLEKGGKSLFP
                     GGELHGLSKEGLAYTSGLLEHSLTGSLLAFTNPSTNSFRRLVPGYEAPISATFAKGSR
                     SAAVRIPGYLKKDEVRVEYRTGDASANIHYMLSAMVLAGVDGILRN"
BASE COUNT          223 a          388 c          388 g          238 t
ORIGIN      
        1 gttccggagg atgccgtcga ctccggcgag caccatcgcc gaaagcatat agtggatgtt
       61 cgccgaggcg tcgccggtgc ggtactccac gcgcacttcg tccttcttca ggtagccggg
      121 gatgcgcacc gccgcgctcc gcgacccctt cgcgaaggtc gcgctgatgg gggcctcgta
      181 gccggggacg agccgccgga agctgttcgt gctggggttc gtgaaggcga gcaggctccc
      241 agtgagactg tgctccagca ggccggaggt gtaggcaagc ccctccttcg acagaccgtg
      301 gagttcgccg cccggaaaga ggctcttccc gcccttttcg aggaactggt ggacgtgcat
      361 gccgctgccg ggcatcttgt agagcggctt cggcatgaag gtcaccttga agcccatctc
      421 ctcggcgacc gagtggatga tccacttggc gatacagacg ctgtcggcgg ccttcaccag
      481 atccatgaag tccagctcga tctcgagctg cgacgcgccc acctcgtggt ggtgatactt
      541 caccgcgata cccgcctcct ccatcagccg gacggtctcg ttccggaagt cgaagaagcg
      601 gtcctcgggc ggcagcttgt ggtacccctt cagcggggcg aaacgctgct gcgtgttgaa
      661 gtcctcgccg agcccttcgc ccgaaccgat ggagtagaag gcgtggtcga gccccgtgga
      721 gtattcgacg gagtcgaaga cgtagtactc aagctcgacg agcatctgcg cgctgtcggc
      781 gagccccttc tccttcaggt actccgcggt gcgctccgcg acggagcggg ggtactggtc
      841 gaagaccttc cgctcggtgg agacgacgtc gcagagcacg tggacgatgc tgaagtcgcc
      901 gcgctcttcc agccacgccg cgttgcagtc cgggatggcg accatgtcgg actggtcgac
      961 cttggcgaag ccgtagttgg acgcgtcgaa gccgatgccg tcgcggaaga tcgcttcgga
     1021 gacgtaccct ctcggaagac tcaccgagcg catgccgccg ccgatgtcga acatcagcat
     1081 gttcagaaaa tccgcttcga tgggaccggg tctaaagcct ttgagtaccg acagtttcat
     1141 tagtattcct ccatatcgct ttattattgt tcggaaaacg cgccgcgccc tccgggaaca
     1201 aaaaaacgct tccgaataca cttccggatt gccccga
//
LOCUS       DAOSWW010000928         1237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_104690_length_1237_cov_2.671743, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000928 DAOSWW010000000
VERSION     DAOSWW010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104690_length_1237_cov_2.671743"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..>1237
                     /locus_tag="PK442_14315"
     CDS             14..>1237
                     /locus_tag="PK442_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO88876.1"
                     /translation="MRSIARVNGQSVVQLRISPRPNSDVVKAGQLLKKELDKLMKTMP
                     DFTAEIPYDDSLFVEASVKNVLRDMGFGILLTSVVLYLFLKRFSATFIVAIAMPVALF
                     ATFTPMVMHGYSMNIMSSLGLAISMGVLVNNAILIIENIFRYRDMGYSPEEAAEKGTV
                     EISLSVLAGTATNLGVFLPVAIMTGIAGQFLVQYAVTIVYATLFSLWVSLTLTPSMAA
                     RFKADAGIPRLGRFLCGWWDWIYSGFETLFLFLLRRAIRHPVLTILVFAGLTFGVFKA
                     GGFIGAEFMPRTDDGAMTINITLPSNTPVWVTQARAKEIEDRIRETLPALKYIVSTVG
                     ASGRNQGTHKATIEAYFHNNVERPSTMDMADQLRPFVNAMPGVDATVSGFRKGMGGGK
                     PIQIIVRGDELETLYR"
BASE COUNT          232 a          375 c          379 g          251 t
ORIGIN      
        1 cagcgaggag gacgtgcgct cgatcgcccg tgtgaacgga cagtcagtgg tgcagctccg
       61 tatcagtccc cgtcccaact ccgacgtcgt gaaggccgga cagctcctga agaaggagct
      121 ggacaaactg atgaagacca tgcccgactt cacggcggag ataccgtacg acgactcgct
      181 cttcgtcgag gcgtcggtga agaacgtcct gcgggacatg ggcttcggta tcctgctgac
      241 gtcggtggtg ctctacctct ttctgaaacg cttctcggcg accttcatcg tcgccatcgc
      301 gatgcccgtc gcgctcttcg cgaccttcac ccctatggtg atgcacggct attcgatgaa
      361 catcatgagc tcgctggggc tcgccatatc catgggtgtc ctggtgaaca acgccatcct
      421 catcatcgag aacatcttcc gataccgcga catggggtac tcccccgaag aggcggcgga
      481 gaaggggacg gttgaaatct cgctctccgt cctcgcgggc acggcgacga acctcggcgt
      541 cttccttccg gtcgccatca tgacggggat cgcggggcag ttccttgtgc agtacgcggt
      601 gacgatcgtc tacgcgacgc tcttctccct gtgggtctcg ctgacgctca ctccctcgat
      661 ggctgcgcgc ttcaaggccg acgcgggaat tccccgcctg gggcgtttcc tctgcggatg
      721 gtgggactgg atttactcgg gcttcgagac gctctttctc ttcttgctcc gaagggcgat
      781 ccggcatcct gtgctgacga tcctcgtctt cgccgggctc acgttcggcg tcttcaaggc
      841 gggcgggttc atcggcgcgg aattcatgcc ccgcacggat gacggagcga tgaccatcaa
      901 tatcacgctg ccgagcaata ctccggtctg ggtgacgcag gcgagggcga aggaaattga
      961 agaccggatc agggaaacgc ttccggcgtt gaaatatatc gtttccaccg tcggcgcgtc
     1021 cggacgaaac cagggaacgc acaaggcgac gatcgaggcg tattttcaca acaatgtcga
     1081 acgtccgtcc actatggata tggccgacca gctgcgcccc ttcgtgaacg cgatgcccgg
     1141 agtggatgcg acagtcagcg gattccgcaa ggggatgggc ggcggaaaac ccattcagat
     1201 catcgtccgg ggagacgagc tggagacgct gtaccgt
//
LOCUS       DAOSWW010000929         1235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_104931_length_1235_cov_3.732203, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000929 DAOSWW010000000
VERSION     DAOSWW010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104931_length_1235_cov_3.732203"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PK442_14320"
     CDS             <1..1162
                     /locus_tag="PK442_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO88877.1"
                     /translation="APGLDELKEALREQQVPVVLAESLSTPLPSFSVRETSVEDESLA
                     VIFATSGTTGLPKAVPLTHGNLLDNALRMYDSLELLAEGDNLLNVLPNFHSFGYTVAG
                     LMPLVRNLKQTILASFLPPVEAMRTIHASGTNVILAVPAMLHFMIIAVEKGAPKPENL
                     KMVVTGGDRLNVQLDAKAKAVLGAGVIEGYGLTECSPVVAVNNNYEKRRLGTVGPVMD
                     GYEWKLLNDKNEDVTASGEGVLWVRGPSVASGYFRDPVMTADRFVDGWFNTGDYVRME
                     EGCVRILDRVTDIIIVGGFNVYPQEVEAILHTHPAVSQAVVVGMPHPVNGEVPKAFIQ
                     LREGMQVTSREIIDFCKKHLAHFKVPRSVEFMESFPLSSTGKVLRRMLKQPK"
BASE COUNT          248 a          365 c          373 g          249 t
ORIGIN      
        1 cgcccccgga cttgacgaac tcaaagaagc gcttcgggag cagcaagtac ccgttgttct
       61 tgccgagtct ctctctacgc cgcttccttc attttccgtt cgtgagacga gcgttgagga
      121 cgagtcgctc gcggtgattt tcgccacgtc cgggacgacc ggtctcccaa aggccgttcc
      181 gttgacccac ggcaaccttt tggacaacgc gctgcggatg tacgattccc tcgaactcct
      241 ggccgagggg gataatctgc tcaacgtgct gccgaacttc cattccttcg gatacacagt
      301 ggcggggctg atgccccttg tccgtaattt gaagcagacg attctcgctt cgttcctgcc
      361 tcctgtggag gccatgagaa ccattcacgc atccggaacg aacgtgattc ttgccgttcc
      421 ggctatgctc cacttcatga ttatcgctgt cgagaagggg gccccgaagc cggagaatct
      481 gaaaatggtc gtcacgggcg gagaccgcct caacgtccag ctcgacgcca aggcgaaggc
      541 cgttctcgga gcgggcgtca tcgaagggta cgggcttacg gagtgctcac ccgtggtggc
      601 ggtgaacaac aactacgaga agcgccggct ggggacagtc gggccggtca tggacggtta
      661 cgagtggaag cttctgaacg acaagaacga agatgtcacc gcctccggag agggcgttct
      721 ctgggtgcgc ggcccctccg tggcaagcgg ctacttccgc gatccggtca tgacggcgga
      781 tcgtttcgtg gacggttggt tcaataccgg cgactacgtg cgcatggaag aggggtgcgt
      841 ccgtatcctt gaccgggtga ccgatatcat catcgtcggc ggattcaacg tgtaccccca
      901 ggaggtcgag gccattctcc acacccatcc ggccgtatcc caggccgtgg tggtgggtat
      961 gccgcacccc gtcaacggcg aggtgccgaa ggcattcatc cagcttcgcg agggaatgca
     1021 ggttacgtcg cgcgagatca tcgacttctg taaaaaacac ctggcgcact tcaaggtgcc
     1081 ccgcagcgtc gagttcatgg agtcgttccc gctctccagc acggggaagg tgctccggag
     1141 aatgttgaag cagccgaagt aaaaaaccgg ggcttcctca atttggggaa gccccggttc
     1201 cctagccgaa aaaaacctca gaatgctcgg aagcc
//
LOCUS       DAOSWW010000930         1232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_105355_length_1232_cov_4.085811, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000930 DAOSWW010000000
VERSION     DAOSWW010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105355_length_1232_cov_4.085811"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PK442_14325"
     CDS             complement(<1..874)
                     /locus_tag="PK442_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOO88878.1"
                     /translation="MNFELSKEHRMARDLFRRFAEKEARPLAQEVDENERFPSETVEK
                     MARSGFLGIPCGKEWGGQGCDTLTYVLCVEELSRVCATTGVVVSAHTSLACDPIEKYG
                     TEAQKRKYLPELLAGRKLGAFALTEPGAGTDASGQQTRAVLEGENYRLNGSKIFITNA
                     GQADVYIVFAMTDKSLGTKGISAFIVEKGFPGFSVGKKELKMGIRGSSTCELIFEDCA
                     VPKENLLGKEGKGFSIAMGTLDGGRIGIAAQALGIAQGALDETVSYVKERKQFGKPIG
                     AFQNTQFRMADMKTR"
     gene            complement(962..>1232)
                     /locus_tag="PK442_14330"
     CDS             complement(962..>1232)
                     /locus_tag="PK442_14330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO88879.1"
                     /translation="GMIGLAKTWAKEFAMKGGNVRVNVIAPGYTMTDILSTVPQELLD
                     KFAAMTMLGRLGRPEEIAKAALFLASDDASYVTGHVLSVNGGMRL"
BASE COUNT          194 a          423 c          359 g          256 t
ORIGIN      
        1 tccgcgtctt catgtcggcc atccggaact gtgtgttctg gaaggcgccg atcggcttgc
       61 cgaactgttt tcgttccttg acgtaggaga cggtctcgtc gagcgctccc tgcgcgatgc
      121 cgagcgcctg ggccgcgatg ccgatgcgcc cgccgtcgag cgtccccatc gcgatggaga
      181 agcccttccc ctctttgcca agcagattct ccttcggcac ggcgcagtcc tcgaagatga
      241 gttcgcacgt cgaggagccg cggattccca tcttcaattc cttcttcccg actgaaaatc
      301 cgggaaagcc cttctccacg atgaaggcgg agatgccctt cgtgccgagg cttttgtcgg
      361 tcatcgcaaa gactatatac acatccgcct gcccggcgtt cgtaatgaag atcttgctgc
      421 cgttcagccg gtagttctct ccttcaagaa ccgccctggt ctgctgcccc gaggcgtccg
      481 taccggcccc gggctcggtg agcgcgaacg cgcccagctt ccgccccgcc aggagctccg
      541 ggaggtactt ccgtttttgc gcctccgtgc cgtacttttc tatcgggtcg caggccagcg
      601 aggtgtgggc ggagacgacg acgccagtgg tcgcgcagac tcggctcagc tcctccacgc
      661 agaggacgta ggtcagcgtg tcgcacccct gtcctcccca ctctttgccg cagggaatac
      721 ccaaaaaacc ggagcgcgcc atcttctcca ccgtctccga cgggaagcgt tcgttttcgt
      781 ccacttcctg cgccagaggc cgcgcttcct tctccgcgaa acggcggaag aggtctctcg
      841 ccattcgatg ttccttgctc agttcaaagt tcatccggta cgcctccgaa agtgccgcgc
      901 cccgcgccga ggctccgcga acgggcccgg cgcggggcgg gtggttgaat agattcgttc
      961 gttacagccg catgccgccg ttcacgctga gcacatgccc ggtgacgtag gatgcgtcgt
     1021 cgctcgccag gaagagcgcc gccttcgcga tctcctcagg ccgcccgagg cgtccgagca
     1081 tggtcatggc ggcgaacttg tccagcagct cctgcggcac ggtgctcaga atgtcggtca
     1141 tggtgtaccc cggagcgatc acgttcacgc ggacgttacc gcccttcatc gcgaactcct
     1201 tggcccaggt cttcgccagg ccgatcattc cg
//
LOCUS       DAOSWW010000931         1232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_105370_length_1232_cov_3.157179, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000931 DAOSWW010000000
VERSION     DAOSWW010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105370_length_1232_cov_3.157179"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PK442_14335"
     CDS             complement(<1..325)
                     /locus_tag="PK442_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO88880.1"
                     /translation="MDYILSIVILAGINMIAVLGLSIFTGFTGLFSLGHAAFVAVGAY
                     TSAILTYYYYVDYYLALAAGAAAAGIVSLIIGVPTLRAKLRSDYFAIAVLGFGEALRV
                     LLENLE"
     gene            complement(328..1200)
                     /locus_tag="PK442_14340"
     CDS             complement(328..1200)
                     /locus_tag="PK442_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO88881.1"
                     /translation="MFLQTLITGLSIGGIYALMAVGYSLVFSVLNFSNFAHGAVIMLG
                     AYVGLALATKFSLGLGLVIAGSVAGAGIIAVLNERLAYSSLRRRKAPSLYLMISAMGC
                     AVFLENMVYATIGSRFYAFPEFFGRQIIDLWGSSVSLLDMGAFLFALAAILGLHLFIS
                     RTRTGIAIRAGVSDMTMCSLMGVDLDRLIAIVFLLAGAFAGVAGVFLGIKYLVYPTMG
                     WVTNKAYIAAVIGGLGSLPGALAGGLILGVVETFVSTYVSSVLRDVFSFTLLIAFLVL
                     RPNGLLGRDGEEKV"
BASE COUNT          292 a          371 c          376 g          193 t
ORIGIN      
        1 tctcaaggtt ttccagaagc accctgaggg cttcgccgaa gccgagcacg gcgatggcga
       61 agtagtcgct tcgaagtttc gccctgagcg tcggcacgcc gatgatcagg ctgacgattc
      121 cggcggccgc agctcccgca gcgagcgcca gatagtagtc gacgtaatag tagtatgtga
      181 ggatggcgga ggtgtacgcg ccgacggcga cgaaggcggc gtgccccagc gagaagaggc
      241 cggtaaaccc ggtgaagatg gaaagcccga gcacggcgat catattgatc cctgcaagaa
      301 taacgatgga gagaatatag tccatggcta gaccttctcc tctccgtcgc gcccgaggag
      361 accgttggga cgcaacacca ggaacgcgat cagcagcgtg aagctgaaga cgtcgcgcag
      421 cacgctggag acgtacgtcg agacgaacgt ctcgaccacg ccgaggatga gccctccggc
      481 aagcgctccg gggaggctgc cgagaccgcc gatcacggcg gcgatgtacg ctttgttggt
      541 cacccacccc atggtcgggt agacaagata ctttatcccg agaaagactc ccgccactcc
      601 cgcgaatgct cccgcgagaa gaaagacgat ggcgatgagc ctgtcaaggt ccactcccat
      661 cagggagcac attgtcatgt ccgagacgcc cgcgcggatg gcgatgcccg tccgcgtccg
      721 gctgatgaag agatgcagcc caagaatggc ggcaagcgcg aagagaaagg cccccatgtc
      781 gaggagactg acactgctcc cccacaggtc gatgatctgc cgtccgaaga actcgggaaa
      841 ggcgtagaaa cgggagccga tggtcgcgta caccatgttc tccaaaaaga ctgcgcaccc
      901 cattgcgctg atcatcaggt agagcgaagg cgcctttctc cgccggaggc tggagtaggc
      961 aaggcgctcg ttgagcaccg cgataatccc cgctccggcg acgcttcccg cgatcacgag
     1021 tcccagcccc aggctgaact tcgtcgccag tgcgagcccc acgtacgccc cgagcatgat
     1081 caccgctccg tgggcaaagt tgctgaaatt caacacgctg aagacgaggg agtaccccac
     1141 ggccatcagg gcatagatgc ctccaatcga gaggccagta atcagagttt gaagaaacaa
     1201 accggctcac acatcctttg agcatattgg gg
//
LOCUS       DAOSWW010000932         1229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_105817_length_1229_cov_2.923339, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000932 DAOSWW010000000
VERSION     DAOSWW010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105817_length_1229_cov_2.923339"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PK442_14345"
     CDS             <1..773
                     /locus_tag="PK442_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOO88882.1"
                     /translation="EIETNVLAHVLREELPEVVLAGATTSGRTTLPALASKLETGLTA
                     DCTGLEMDLETGLLLQTRPAIGGNVMATIKTPERRPQMATVRPRTFRIPEPSERKGEL
                     LTPGIPAALFESSIENLGFDRVEDGAANIADMDVVVSGGKGLKRPESFRMLEELAELL
                     GGGVGASRPTVEAKWMDYPHQVGLSGKVVAPKVYIAAGISGAVQHLAGMQTAGYIVAI
                     NRDPDASIFRVADLGLRGDLFDIIPRIIDRIKTEVNSR"
     gene            770..>1229
                     /locus_tag="PK442_14350"
     CDS             770..>1229
                     /locus_tag="PK442_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOO88883.1"
                     /translation="MSVQAYSPVTEQTVEELQKLIGASNVSADLEKRIAYSYDEVPRF
                     YWDKEYIAEVVVFPETTEDVSAVMRYANDRRIPVTPRGAGTGLSGGAVPFSGGIVLSF
                     EKMRAIIELDKKNLTITVEPGVVTAEITKAAEREGLLYAGDPCSGDASFIG"
BASE COUNT          239 a          366 c          413 g          211 t
ORIGIN      
        1 aagagattga gacaaacgtc ctcgcccacg tgcttcgcga ggagctaccc gaggtagtgc
       61 tcgccggtgc gacgacgtcc ggacggacga cgctccccgc attggcctcg aaactggaga
      121 cggggctcac cgcggactgc accggccttg agatggacct cgaaaccggg ttgctgcttc
      181 agacccgccc ggctatcggc ggcaacgtca tggcgaccat caagacgccg gagcgccggc
      241 cgcagatggc gacggtgcgc ccgcggacgt ttcggattcc ggagccttcc gagcggaagg
      301 gggagttgct gacgcccggg attcctgccg ctctcttcga gagttccatc gagaacctcg
      361 gtttcgaccg ggtggaggac ggtgcggcga acatcgcgga tatggacgta gtcgtctcgg
      421 gaggcaaggg gctgaagcgc cccgagagct tccggatgct cgaagagctt gcggagcttc
      481 tcggcggggg cgtcggggcc agccgtccga cggtggaggc caagtggatg gactatccgc
      541 accaggtcgg gctctcagga aaggtcgtcg ccccgaaggt ctacatcgca gccgggattt
      601 ccggagccgt gcagcatctc gcggggatgc agaccgccgg gtacatcgtt gcgatcaacc
      661 gcgacccgga cgcctccatc ttccgggtgg cggatctggg gcttcgaggg gatcttttcg
      721 atatcatccc ccggattatc gatcgtatca aaacggaggt gaactccaga tgagtgtcca
      781 agcatactca ccggtcactg aacagacagt agaggaactc caaaagctga taggagcgtc
      841 gaacgtctcc gcagaccttg aaaagcgcat cgcctactcc tatgacgaag tgcccaggtt
      901 ttactgggac aaggagtata tcgccgaggt cgtcgttttc ccggagacta ccgaggatgt
      961 ctccgcggtg atgcgctacg cgaacgaccg caggattccg gtgacgccaa gaggggccgg
     1021 cacaggcctc tccggcggcg cggttccttt ctcgggcggg atcgtcctct cgttcgagaa
     1081 gatgcgggcc atcatcgagc tggacaagaa aaacctcacg atcacggtcg aacctggcgt
     1141 ggtgacggcg gagatcacga aggcggcgga gcgtgaaggg ctgctctacg ccggggaccc
     1201 gtgcagcggc gacgcctcct tcatcggcg
//
LOCUS       DAOSWW010000933         1228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_106024_length_1228_cov_2.243819, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000933 DAOSWW010000000
VERSION     DAOSWW010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106024_length_1228_cov_2.243819"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..>1228)
                     /locus_tag="PK442_14355"
     CDS             complement(103..>1228)
                     /locus_tag="PK442_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO88884.1"
                     /translation="LEESIGEDALIDLIAHVDKVFVEYGADNHAYEPTGEIVRDTIRK
                     ASSVGIKILPARIRHIGTDASREVLNNMYEALKPKCDIMMGTFVEDLLIENGRACGVV
                     LADGTVHRSASVIVAPGREGASWLAGVVAKLNIPIASMPVDIGVRVEVPDSVCEDLTR
                     HFYEIKCLYNTPTFDDRCRTFCMNPSGFVVYEYNQAHDLVTVNGHSLKNVKSRNTNFA
                     ILVTKNFTQPFNDPIGYATHVAKLANMLAGGGILVQRLGDLRDGRRSTQSRIDRGMIT
                     PTANAQPGDLSLVLPHRYLTDVVEFLDGLNSIMPGVNQNDTLLYGVEIKLYSLRLELR
                     KNLEVPVIERLFMAGDGAGVSRGIIQAAASGVVAARGALA"
BASE COUNT          214 a          380 c          373 g          261 t
ORIGIN      
        1 cggcgcctcc ttcttcgccg gagcaaagtg aagtacctct tccacacgaa aatcgtcggg
       61 agctgtcttg attcgcatct gtcttctcct ctgctgcggg cgctaagcca gcgcgccgcg
      121 cgcggcgacg acgccgctgg ccgcggcctg tatgattccc cggctgacgc ccgcgccgtc
      181 gccggccatg aagagacgtt cgatgaccgg cacctccagg ttcttccgaa gctccagccg
      241 gagcgagtac agcttgatct ccaccccgta gagcagcgtg tcgttctggt tcacgccggg
      301 catgatggag ttgagaccgt cgagaaattc cacgacgtcg gtcagatagc ggtgcggcag
      361 cacaaggctg aggtcgccgg gctgcgcgtt tgccgtcggg gtgatcatcc cgcggtcgat
      421 gcgcgactgg gtggaacgcc gtccgtcccg gagatcgccg agccgctgga cgagaattcc
      481 gcctccggca agcatgttcg ctagttttgc gacgtgcgtg gcgtacccta tagggtcgtt
      541 gaacggctgc gtgaagttct tcgtcaccaa aatggcgaag ttcgtgttcc tggacttcac
      601 gttcttcagg ctgtgcccgt tcacggtgac gagatcgtgc gcctggttgt attcgtagac
      661 gacgaatccc gacggattca tgcagaaggt gcggcagcgg tcgtcgaagg tcggcgtgtt
      721 gtacagacac ttgatctcgt agaagtgccg tgtgaggtct tcgcagacgc tgtccggaac
      781 ttccacccgc acgccgatat ccaccggcat ggaggctatc gggatgttca gcttcgcgac
      841 gacgcctgcc agccaggaag ccccctccct acccggggcg acaatgacgc ttgcgctccg
      901 gtggacggta ccgtcggcga ggacgacgcc gcaggcgcgt ccgttctcta tcaggagatc
      961 ctcgacgaac gttcccatca taatgtcgca cttcggcttc agcgcctcgt acatattgtt
     1021 gagcacttcg cgcgaggcgt ccgtgccgat atggcggatc ctcgcgggca gaatcttgat
     1081 cccgacgctg gatgctttcc tgatagtatc tcggacaatt tcacccgtcg gttcgtaggc
     1141 gtggttatcg gcgccgtact caacgaaaac cttgtccacg tgcgcaatca ggtcgatcag
     1201 cgcatcctcg ccgatactct cctcaagg
//
LOCUS       DAOSWW010000934         1227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_106121_length_1227_cov_3.131399, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000934 DAOSWW010000000
VERSION     DAOSWW010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106121_length_1227_cov_3.131399"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..>1227)
                     /locus_tag="PK442_14360"
     CDS             complement(283..>1227)
                     /locus_tag="PK442_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOO88885.1"
                     /translation="VPCTPRQFEFARLNLTYTVMSKRLLLELVNRKIVSGWDDPRMPT
                     ISGIRRRGYTPSALRRFCAEIGVSKSNSMVDIEFLQFIIREELNATSLRAMAVLDPLK
                     VVVENYPEGQVEMLDAENNPEDPNAGTRQIPFSRELYIEREDFMENPPKKFFRLSPGS
                     EVRLKHAYFITCKEVVRNDAGEIVELRCEYDPLTRGGEAPDGRRVKGTLHWVSVPHAV
                     KAQVRLYENLFTLRDMSEMEEGKSYNDYLNPESLTVLENCFVEPALAAAKPLDRFQFL
                     RQGYFCADADSTPDRPVFNRTVALKDSWAKMQQKGQAD"
BASE COUNT          234 a          413 c          331 g          249 t
ORIGIN      
        1 aacctggcga acagagacat attcagggac aaacgagcgg catccggggc aaaagcggaa
       61 gccgttctcc acccgtctcc cgcattccgg acaaaggcgg tcgccgcccc gttcttcttt
      121 tccgggaatc ccggagttgt ccatattctt catgtacagg aaacctcctc tcgaatccac
      181 ggaaaaacgc ctttacagag tataccgtgt accatttcta ttctatcatt tcccaggact
      241 gcgtttcctc cgtcaaatac cggaacatgg aggctgaaag actcagtcgg cctgcccctt
      301 ctgctgcatt ttcgcccacg agtccttcag cgcgaccgtc ctgttgaaca ccggccggtc
      361 aggcgtcgag tcagcgtccg cgcagaagta cccctggcgg aggaactgga agcggtcaag
      421 cggcttcgcc gctgcgagcg ccggttcgac aaagcagttc tcaagcaccg tcagcgactc
      481 cggattcaga tagtcgttgt agctcttccc ctcctccatc tcgctcatat cgcgcagcgt
      541 gaagaggttc tcgtagagcc gcacctgcgc cttcaccgcg tgcggaaccg agacccagtg
      601 cagcgtcccc ttcacgcggc ggccgtccgg cgcttcgccc cccctggtaa gcgggtcgta
      661 ctcgcagcgc agttcgacga tctccccggc atcgttccgg acaacctcct tgcacgtgat
      721 gaagtacgcg tgcttcagcc ggacttcgct ccccggagag agacggaaga actttttcgg
      781 cgggttctcc atgaaatcct cccgctcgat gtagagctcc ctggaaaagg ggatctgcct
      841 cgtcccggcg ttcggatcct ccggattgtt ctcggcgtcg agcatctcga cctgcccctc
      901 ggggtagttc tcgacgacca ccttcagcgg atcgagcacc gccatcgccc gcagcgacgt
      961 cgcgttcagc tcctccctga tgatgaactg aaggaactcg atatccacca tgctgttcga
     1021 cttcgacacg ccgatctccg cgcagaaacg ccggagcgcc gacggcgtgt acccccttct
     1081 ccggatgccg ctgatcgtgg gcatccgcgg gtcgtcccag ccggagacga tcttccggtt
     1141 caccagttcc agcagaaggc gcttgctcat cacggtgtac gtcagattca accgggcgaa
     1201 ctcgaactga cggggcgtgc acggaac
//
LOCUS       DAOSWW010000935         1226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_106247_length_1226_cov_4.038429, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000935 DAOSWW010000000
VERSION     DAOSWW010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106247_length_1226_cov_4.038429"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PK442_14365"
     CDS             <1..333
                     /locus_tag="PK442_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO88886.1"
                     /translation="IVNLRGQIVPIVDLCKRFLVVDREGRNDIDRRIIVVHMAGQNIG
                     ILVDGVSEILRVPDEAIEPTPPMVASGASVDFIRGVAKVKDRLIMALDLDRIFSTEER
                     ELLAQTES"
     gene            411..866
                     /locus_tag="PK442_14370"
     CDS             411..866
                     /locus_tag="PK442_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88887.1"
                     /translation="MSRIEKTPTIMIVDDTPAILDLLDQMLRAQGYRALTFPRGKLAL
                     EAAEETPPDLILLDITMPEMDGFEICRRLKNTPRLAEIPVIFIGPLAEADEKARAFAA
                     GGADYVTKPFQEEEILVKVRTYLRMMEMQRELRKREKTTGTPSGEKAPL"
     gene            1007..>1226
                     /locus_tag="PK442_14375"
     CDS             1007..>1226
                     /locus_tag="PK442_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOO88888.1"
                     /translation="MGSMLEQHILRTPLIGYSFRRIILDDSGKPVDYEFLEVNDSFER
                     FTGLARADILGRTASKVVPGIESASFDWL"
BASE COUNT          276 a          350 c          383 g          217 t
ORIGIN      
        1 atcgtcaacc tccgggggca gatcgttccc atcgtcgacc tgtgcaagcg ttttttggtc
       61 gtcgacaggg agggaagaaa cgatatcgac cggcgtatca tcgtcgtcca catggcgggg
      121 cagaacatcg gcatcctggt ggacggcgtg tcggagatac tgcgcgttcc ggacgaggcc
      181 atcgagccga cgcccccaat ggtggcgagc ggcgcaagcg tggacttcat ccgcggagtg
      241 gcgaaggtga aggatcgttt gatcatggcg ctggatctcg accgtatctt ctcaacggaa
      301 gagagggagc tgctcgccca gaccgagagc tgagagaacg cgggggagga cgctccccgc
      361 tcccccgcgt tgctccgccg cctggcaggc gagaggaggt acagagggaa atgagccgca
      421 tcgaaaagac gccgaccatc atgatcgtgg acgatactcc cgctattctc gacctgctgg
      481 atcagatgct gcgggcgcag gggtaccgcg ccctgacctt tccgcgagga aaactggcgc
      541 tcgaagccgc ggaggaaacc cctccggatc tgatcctgct cgatatcacg atgcccgaaa
      601 tggacggatt cgagatctgc aggcgattga aaaacacgcc tcgtctcgcg gagattccgg
      661 tgatcttcat cgggccgctc gccgaagccg acgaaaaggc ccgagctttc gccgccggcg
      721 gcgcggacta cgtaacgaag ccttttcagg aggaggaaat tctggtaaag gtacggacat
      781 atcttcggat gatggagatg cagcgcgagc ttcgaaagcg ggagaagaca acgggaacgc
      841 cctccggaga aaaagcgccc ctgtaagctc cgcaattcga agaaaaatag aatgtgcacg
      901 ccgctgcgct gatacaatag aatcatgaaa aaagaaacgg cgagccgtac tctttccggc
      961 gcgccgtttc cgccgaagca ggccaacgcg gcacggaggt gtattcatgg gttcgatgct
     1021 cgaacagcac atactgcgca cccctctgat cgggtactca tttcgccgga tcatcctcga
     1081 cgattcgggc aagccggtcg actacgaatt tctggaggtg aacgacagct tcgagagatt
     1141 caccgggctt gcgcgcgccg atattctggg gcgcactgca agcaaggtcg ttcccggcat
     1201 cgaaagcgcc tccttcgact ggctcg
//
LOCUS       DAOSWW010000936         1225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_106409_length_1225_cov_2.967521, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000936 DAOSWW010000000
VERSION     DAOSWW010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106409_length_1225_cov_2.967521"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PK442_14380"
     CDS             complement(<1..202)
                     /locus_tag="PK442_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88889.1"
                     /translation="MSTGAPGEKHLPERGEAAAEAIPAGLSGVKMTPMLAQYFEWKKQ
                     YPDCILFFRMGDFYEMFFDDAVK"
     gene            complement(199..621)
                     /gene="ruvX"
                     /locus_tag="PK442_14385"
     CDS             complement(199..621)
                     /gene="ruvX"
                     /locus_tag="PK442_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOO88890.1"
                     /translation="MLSRRTLALDIGTVRIGVAVSDPLGMFAQGIAVLAAASAWMEEL
                     DALVKTYDPETLLLGVPLRTNGARGPEAEKIEALGRVLGERYPSVKVVLHDERFSTVM
                     ATQALLEGDVSRKNRKGKVDKVAAALILQSWLDRSGGV"
     gene            complement(615..>1225)
                     /locus_tag="PK442_14390"
     CDS             complement(615..>1225)
                     /locus_tag="PK442_14390"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOO88891.1"
                     /translation="FKILREEGIGSGTRRITAATGLEALRVFQRTWRTLGEIGGALAV
                     DETGVLPKIEQLQSEVKSLARTIEEMKINSLSSSLESSLRHASAGGVDVVFGRFDGVN
                     ADMLREMGDRVLNKRSVDTGVLVALASVAEGNVTIIAMADEKALARGVSAGKVVKEGS
                     AMLGGGGGGRPALAQGGGKKPEALEDFFKALPGIVERQLLSC"
BASE COUNT          219 a          459 c          322 g          225 t
ORIGIN      
        1 cctttaccgc gtcgtcgaag aacatctcgt agaagtcccc catccggaaa aagaggatgc
       61 agtccggata ctgtttcttc cactcgaagt actgcgcgag catcggcgtc atcttcaccc
      121 cggacagtcc tgcgggaatg gcctcggcgg ccgcctcccc gcgctccggc aggtgtttct
      181 ctcccggagc ccctgtgctc ataccccgcc gctcctgtcc agccagctct gcagaatcag
      241 cgccgccgcc accttgtcca cctttccctt ccggttcttc cgggaaacgt ccccttcaag
      301 aagcgcctgc gtcgccatga cggtggagaa ccgttcgtcg tgcaaaacaa ccttcaccga
      361 cggataccgt tctcccagca cacgccccaa cgcctctatc ttctccgcct ccggcccgcg
      421 cgcgccgttc gtgcgcagcg gcacacccag cagcagcgtc tcgggatcgt acgtcttcac
      481 aagcgcgtcc agctcctcca tccaagcgga tgcagcagcc agaacggcga tcccctgggc
      541 gaacatcccc aggggatcgc tgactgcgac gccgatgcgg accgtcccta tgtcaagggc
      601 gagcgtccgt ctgctcagca tgaaagaagc tgccgctcca caatgccggg aagcgcttta
      661 aagaagtctt ccagagcttc cggcttcttg ccgccgccct gcgcgagcgc cggtcttccg
      721 ccgccgccgc cgccgagcat cgccgaacct tccttgacaa cctttccggc ggacacgccc
      781 ctggcaagcg ccttctcgtc ggccatggca ataatggtca cgttcccctc cgctacggag
      841 gcaagagcga ccagcacgcc cgtatcaacg gaacgcttgt tgagcaccct gtcgcccatc
      901 tcgcgcagca tatccgcgtt cacaccgtcg aaacggccga agaccacgtc cacgccgccg
      961 gccgaagcat gccggagcga actttcgagc gagctgctca gcgagttgat tttcatctct
     1021 tcgatcgtcc gggcgagcga cttcacctcc gactgaagct gttcgatctt cggcagcacg
     1081 cccgtttcat caacagcgag cgcgcctccg atctcgccca gcgtccgcca ggtacgctgg
     1141 aagacgcgga gcgcctccag tccggtcgcc gcggtaatcc ttcgggttcc ggagccgatg
     1201 ccctcctcgc gcagaatctt gaaca
//
LOCUS       DAOSWW010000937         1224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_106578_length_1224_cov_2.741660, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000937 DAOSWW010000000
VERSION     DAOSWW010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106578_length_1224_cov_2.741660"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PK442_14395"
     CDS             complement(<1..473)
                     /locus_tag="PK442_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO88892.1"
                     /translation="MKLRRIELKGFKSIDGRTGQTIPFGDITVMLGANGSGKSNIVSF
                     FKMLNFMMTGAFQSYVGRQGVQQLLFYGPRTTESIIFSLFFSSEEADDTYEARLSYGL
                     PDRLFIGGEKVTYLKKGETSAQEFFLESGGAEVRPRRSPRNRPPAWAPLRGVLRPR"
     gene            complement(644..>1224)
                     /locus_tag="PK442_14400"
     CDS             complement(644..>1224)
                     /locus_tag="PK442_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO88893.1"
                     /translation="FWRGLVLLAADGLAIALPELRGETLHFLLAGRIGAWGVPAQALW
                     AVALALLLAFLLNRRPFGDAILFIGDNRNTAEMMGIPVGRVRIAVFALMGLLAAFAGI
                     LATFELNNWWPTQGEGYMLMVFASVFVGGTSAYGGEGTIYGSFIGAIIIGIIEAGIVS
                     AGLVGFWTRLVHGLVILLSVSVYALLARRRAR"
BASE COUNT          250 a          386 c          351 g          237 t
ORIGIN      
        1 cggggccgaa gtactccacg aagaggcgcc cacgcaggtg gtcgatttcg tggcgaccga
       61 cgtggacgaa cttccgcacc gccgctttcc agaaagaact cttgagcgga ggtctcgcct
      121 ttctttaaat aggtgacctt ttcaccgccg atgaacagac ggtccggaag gccgtaggag
      181 agtctcgcct cgtaggtgtc gtccgcctcc tccgaagaga aaaagaggga aaaaataatc
      241 gactcggtcg ttcggggtcc gtagaagagc aattgctgaa ctccctgtct tccaacatag
      301 ctctgaaaag ctccagtcat cataaaattc agcattttga agaacgagac aatgttgctc
      361 ttcccggagc cgttcgcgcc gagcatcacg gtgatgtcac cgaacggaat cgtctgtccg
      421 gttcgtccgt cgatcgattt gaagcccttc agttcgatac gccgtagttt catagaactc
      481 ccctccgcag cgcgctctct attcagtctc tcggtgcggg acgatacgcg cccagtcttt
      541 tctatcatat ttcccggtgt ccggcaaggc gtaccgcctt ttaccgcgat gcctttttca
      601 cgatgttcct ccgcttttac acttcgttta tgacgattct tcgctacctc gccctcctgc
      661 gcgcgagcag cgcgtagacc gagacggaga gaaggatgac gaggccgtgg acgaggcgcg
      721 tccagaatcc gaccagcccc gcgctcacga ttcccgcctc gatgatgccg atgatgatcg
      781 cgccgatgaa ggaaccgtag attgttccct cgccgccgta ggccgaggtg ccgccgacga
      841 agacggaggc gaagaccatc agcatgtacc cctccccctg cgtgggccac cagttgttca
      901 gctcgaaggt ggccaggatt ccggcgaacg cggcgagcag tcccatcagg gcgaagaccg
      961 cgatgcggac ccgtccgacc ggaatgccca tcatctcggc ggtgttgcgg ttgtcgccga
     1021 tgaagaggat cgcgtcgccg aacggacgcc tgttgagcag aaacgcgagc agtagggcga
     1081 gcgcgacggc ccacaaggcc tgcgcgggga ctccccacgc gccgatcctt cccgcgagca
     1141 ggaagtgcag cgtctctccc ctcagctcgg ggagcgcgat cgccagaccg tccgccgcga
     1201 gcagcaccag ccctctccag aaga
//
LOCUS       DAOSWW010000938         1223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_106700_length_1223_cov_2.939212, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000938 DAOSWW010000000
VERSION     DAOSWW010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106700_length_1223_cov_2.939212"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PK442_14405"
     CDS             complement(<1..832)
                     /locus_tag="PK442_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88894.1"
                     /translation="MKRNGMKATVMVVDDTPANLDLLDRMLRAAGYRVLAFPRGRLAL
                     EAAIEIPPDLIVLDVLMPDMDGFEVCRRLKATPRLASVPVLFISALGDTSDKLRAFAC
                     GGADYITKPFQEEEVLARAGAHLEIAALQRRLREQNENLERLVAERTQELASERARAL
                     RSFAERIRGARWGEERSLGLDAAVSEFDALSEAIEELAEKTYTREELRARYIGDMIRA
                     EEKGRKRFARELHDGPLQSAAAAIRRVQMAREAAAANPSGELGALLDTAEEIMRSAAD
                     E"
     gene            complement(829..>1223)
                     /locus_tag="PK442_14410"
     CDS             complement(829..>1223)
                     /locus_tag="PK442_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88895.1"
                     /translation="FAFEEDEQKAFQAGVNGYLRKPFRPDELFLALGKALGLRCIHAE
                     DSRGGETPLTSEALTVIPAEMRAAMRAAVKSGDMAGLREQITAARAIDEATAEKLLAL
                     ARRYDYERLQSLLLSVPQCETNEGRTRE"
BASE COUNT          186 a          420 c          368 g          249 t
ORIGIN      
        1 gctcgtccgc cgcactcctc atgatctcct ccgcagtatc cagaagagcg ccgagttcgc
       61 cggacggatt cgccgctgcg gcttccctcg ccatctgaac tcgtctgatc gcggcggcgg
      121 ccgactgaag cggtccgtcg tgcagctcgc gcgcaaagcg tttgcgcccc ttctcctcgg
      181 cgcggatcat gtcgcctatg taccgcgccc gcagctcctc cctggtgtat gtcttttcgg
      241 ccagctcttc gatggcttcc gacagcgcgt cgaactcgga gactgcggcg tcgagcccca
      301 ggctgcgctc ctcgccccat cgcgctccgc gtattcgctc cgcgaaactc cgcagcgcgc
      361 gggcgcgctc cgaagcgagt tcttgtgtac gctcggcgac cagccgctca aggttctcgt
      421 tctgctcccg cagtcgacgc tgcagcgccg ctatttcgag atgcgcgccc gcgcgcgcca
      481 gcacctcttc ctcctggaac ggttttgtga tgtaatcggc ccctccgcag gcgaaggcgc
      541 ggagcttgtc cgacgtgtcg cccagcgcgc tgatgaagag cacgggaacg gaggcgagcc
      601 ggggggttgc tttcaagcgg cggcagacct cgaagccgtc catatcaggc atcaaaacat
      661 cgagcacgat gagatccggc gggatctcga tcgccgcttc cagcgccagc cgcccgcggg
      721 ggaaggcgag cactctgtat cccgcagccc gcagcatacg gtcgagaagg tcaaggttgg
      781 cgggcgtatc gtccaccacc atgacggtcg ctttcattcc gttccgcttc attcccgtgt
      841 tctcccctcg ttcgtctcgc actgtggtac agataaaaga agcgattgca gcctctcgta
      901 gtcgtaccgt ctggcaagcg ccagaagttt ttcggcggtt gcttcgtcga tcgcgcgcgc
      961 ggcggtgatc tgttctcgca agcccgccat gtcgcccgac ttgactgctg cgcgcatcgc
     1021 ggcgcgcatt tctgcgggaa tgacggtcag cgcttcgctc gtcagcggcg tttcacctcc
     1081 gcgggagtcc tcggcgtgta tgcaccggag tccgagagcc tttcccagcg caagaaagag
     1141 ctcgtccggc ctgaacggtt tgcgaaggta gccgttcact cccgcctgaa acgctttctg
     1201 ttcgtcctcc tcgaaggcga agg
//
LOCUS       DAOSWW010000939         1223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_106732_length_1223_cov_2.483733, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000939 DAOSWW010000000
VERSION     DAOSWW010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106732_length_1223_cov_2.483733"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK442_14415"
     CDS             <1..357
                     /locus_tag="PK442_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOO88896.1"
                     /translation="EEDSRGGKFVEVKKSHVGKGSKVPHLAYIGDAEIGAETNIGAGT
                     ITCNYDGKKKNPTTIGNRCFIGSDTMLVAPVNVGDDGFTAAGSVITQDVPEGALAVGR
                     AKQRNIEGWAKRAGKK"
     gene            384..>1223
                     /locus_tag="PK442_14420"
     CDS             384..>1223
                     /locus_tag="PK442_14420"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOO88897.1"
                     /translation="MGTIGRELKVFSGTAHPAFARRVCSELGIELSRAKHFRFSDGEI
                     GLSIEESVRGADVFVVQPTCNPVNENLVELLIMIDALKRASAYRINVVIPYFGYARQD
                     RKTKPRDPISAKLMANLLTEAGATRVISADLHAGQIQGFFDIPVDNLTGIPLLASYFK
                     EILAECVECGSLTVVAPDVGGVVRARKFAGTLKADLAIVDKRRSHEVANLCEVMDVIG
                     DIRGKTAILVDDIIDTAGTICNAAGLLKERGAVKIYACSTHGVLSGPALDRLEHSDID
                     EVVL"
BASE COUNT          270 a          328 c          403 g          222 t
ORIGIN      
        1 gaagaggact cacggggcgg aaagttcgtc gaggtgaaga aaagccatgt cggcaaaggg
       61 agcaaagtgc cccacctggc atacatcggc gacgcggaga tcggcgccga gaccaatatc
      121 ggcgccggga cgatcacttg caactacgac ggaaaaaaga agaaccccac gaccatcggc
      181 aaccgctgtt tcatcggaag cgatacgatg cttgtggcgc ctgtcaacgt cggcgacgac
      241 ggctttaccg ccgcgggttc ggtaattacg caggacgttc ccgaaggggc gcttgcggtc
      301 ggcagggcga aacagagaaa cattgaaggc tgggcgaaac gagcaggaaa aaagtgaaat
      361 gattgaagga ggcatccgtg atgatgggaa ccatcgggcg tgagttgaag gtcttttccg
      421 gaacagccca tcccgccttc gcacgaaggg tatgcagcga gcttggaatt gaactgtcca
      481 gggcgaagca cttccgcttt tcggacggag agatagggct ctccattgag gagagcgttc
      541 ggggcgcgga tgtgttcgtg gtgcagccga cgtgcaatcc tgtcaacgag aatctcgtcg
      601 aattgctgat tatgatcgac gcgctcaagc gggcttcggc gtaccggatc aacgtcgtga
      661 tcccctactt cggctacgcg cggcaggaca ggaagacaaa gcccagggat cccatctccg
      721 cgaagctcat ggcgaacctg cttaccgagg cgggggccac gcgtgttatt tcggcggatc
      781 ttcacgccgg gcagatacag ggatttttcg atatccctgt ggataacctg acggggattc
      841 ccctgctcgc gagctacttc aaggagatcc tggcggagtg cgtcgaatgc ggttccctca
      901 cagtagtggc gccggacgtg ggcggcgtgg tccgtgcaag gaagttcgcc ggaacgctga
      961 aggcggatct cgccatcgtc gacaagaggc gttcgcacga ggtcgcgaac ctgtgcgaag
     1021 ttatggacgt catcggcgat atccgcggga agacggcgat cctggtcgac gatatcatcg
     1081 acactgcggg gaccatctgc aatgccgcgg ggctgctcaa ggagcgcggc gccgtgaaga
     1141 tctacgcctg ctcgacgcac ggcgtcctct cggggccggc tctcgaccgt ctggagcact
     1201 ccgatatcga cgaagttgtg ttg
//
LOCUS       DAOSWW010000940         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_106837_length_1222_cov_3.053985, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000940 DAOSWW010000000
VERSION     DAOSWW010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106837_length_1222_cov_3.053985"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1222
                     /locus_tag="PK442_14425"
     CDS             <1..>1222
                     /locus_tag="PK442_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOO88898.1"
                     /translation="TKVNSRIFLRRRKEWGHTLGDAEGKWIETSPGRVIFNSRLPEPL
                     RFINRQLGKKDLGQLLDRAFDLVDRVSMVTMLDNIKSLGYHWATVSGISFGVQSIIIP
                     PEKRELTAKAMEQDEELKSEYNMGILTPDEYLFQKENLWSETSRAIADSIVDHMDRTN
                     PIRMMSDSGARGSRSQMGQMAGIRGLMSDPSGRIIDYPITANFREGMNMLEYFISTHG
                     ARKGLADTALRTAKSGYLTRRLVDVAQDLIITQHDCGTNQGVLIRPLTQDGKVMIPLS
                     ERIYGRTSLHDIEHPETGGMIVASGECITDEKARMIEKSGIEEVWVRSPLSCALRHGI
                     CQVCYGMDLATRKEVPMGEAVGVVAAQSIGEPGTQLTMRTFHTGGVRLTGEDITQGLP
                     RIEQLFEVRRPKKVA"
BASE COUNT          266 a          379 c          388 g          189 t
ORIGIN      
        1 caccaaggtg aacagccgca tcttcctccg gcgtcgcaag gagtgggggc ataccctcgg
       61 agatgcggag ggcaaatgga tcgaaacctc tccggggcgc gtcatcttca acagccggct
      121 ccccgagccg ctgcgcttca tcaacaggca gctcggcaag aaggacctcg gacagctgct
      181 cgacagggcg ttcgacctcg tcgacagagt ttccatggtg acgatgctcg acaacatcaa
      241 gtcgctgggg taccactggg cgacggtcag cggcatcagc ttcggcgtgc agtcgatcat
      301 catcccgccg gaaaagcgcg agctcacggc gaaggccatg gagcaggacg aagagctgaa
      361 gtcggagtac aacatgggta ttctcacccc ggacgagtac ctcttccaga aggagaacct
      421 gtggtcggag acgtcgcgcg ccatagccga cagtatcgtc gaccacatgg acaggacgaa
      481 tcccatccgc atgatgagcg actccggagc gcggggaagc cgcagtcaga tggggcagat
      541 ggccggaatc cgaggcctga tgtccgaccc ttcgggccgc atcatcgact acccgatcac
      601 cgcgaacttc cgcgagggga tgaacatgct cgagtacttc atctcgacgc acggcgcccg
      661 aaagggactt gccgacaccg ccctcagaac cgccaagtcg gggtatctta cccgccgcct
      721 ggtggacgtg gcgcaggacc tgatcatcac gcagcacgac tgcggaacga accagggtgt
      781 gctcatccgc cccctgacgc aggacggaaa ggtcatgatt ccgctttccg agcgtatcta
      841 cggcagaacg tcgctgcatg atatcgaaca tccggagacc ggcgggatga tcgtcgcgtc
      901 gggcgagtgc atcacggacg agaaggcgcg gatgatagag aaatccggca ttgaagaggt
      961 ctgggtacgc agtccgctga gctgcgcgct ccgccacggc atctgccagg tctgctacgg
     1021 catggacctg gccaccagaa aggaagtgcc gatgggagag gccgtcggcg tcgtcgcagc
     1081 gcagtccatc ggcgaaccgg gaacgcagct cacgatgcgg accttccaca ccggcggcgt
     1141 ccgtctcacg ggagaggaca tcacgcaggg tcttccgcgc atcgaacagc tcttcgaggt
     1201 ccgccgtccg aagaaggtcg cc
//
LOCUS       DAOSWW010000941         1221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_106958_length_1221_cov_3.884220, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000941 DAOSWW010000000
VERSION     DAOSWW010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106958_length_1221_cov_3.884220"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK442_14430"
     CDS             <1..193
                     /locus_tag="PK442_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88899.1"
                     /translation="HGMDDGFGFAEGEWLAGWIALRFLNDQEVARGHFERMYKAVNYP
                     ISRARSGPAAAGDASGGGA"
     gene            190..>1221
                     /locus_tag="PK442_14435"
     CDS             190..>1221
                     /locus_tag="PK442_14435"
                     /inference="COORDINATES: protein motif:HMM:NF017039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOO88900.1"
                     /translation="MNASAPGEKNLPERWEAAVEAIPAGLSGVKMTPMLTQYFEWKKQ
                     YPDCILFFRMGDFYEMFFDDAVKASAILDITLTARDAEKSIPMAGVPWHAVNGYLGRL
                     VRAGCKVAICEQITEPSNKGIVERKVIRIVTPGTYVPEEAGSGGRLAAVCRAGKDLAV
                     ALLSVETGRLEAGVFPKGEAAALLGSFAPGELLHPASLSLSEWFPLAGDYFPLPREDE
                     FFSPVQGTRRLLQQWKTATLASFGLEDGSPAAGAAAAVLAYLHETQFGAVEYIKRAHP
                     LRSSEHLHLDVTTGRNLELFDTRQEQNGDAPSLYRTLNRCRSTMGRRLLREWLLRPLL
                     DLGELRRRQD"
BASE COUNT          199 a          379 c          438 g          205 t
ORIGIN      
        1 gcacggcatg gacgacggct tcggcttcgc cgagggcgag tggctggccg gctggatcgc
       61 cctgcgcttc ctcaacgacc aggaggtggc gcgcggccat ttcgagcgca tgtacaaggc
      121 ggtcaactac cccatcagcc gcgcccgctc cggcccggct gcggccgggg atgcgtctgg
      181 aggcggggca tgaacgcgtc ggctccggga gagaaaaacc tgccggagcg ctgggaggcg
      241 gccgtcgagg ccattcccgc cgggctgtcc ggggtgaaga tgacgccgat gctcacgcag
      301 tacttcgagt ggaagaaaca gtatccggac tgcatcctct tcttccggat gggcgacttc
      361 tacgagatgt tcttcgacga cgcggtaaag gcgtcggcga ttcttgatat aaccctcaca
      421 gcgcgggacg cggagaagag tatcccgatg gcgggcgtgc cgtggcacgc ggtcaacgga
      481 tacctgggac ggcttgtccg cgcagggtgc aaggtggcca tctgcgaaca gatcaccgag
      541 ccgagcaaca agggcatcgt cgaacggaag gtgatccgca tcgtcacgcc ggggacgtac
      601 gtccccgagg aggccggatc gggcggaagg ctggctgccg tgtgccgggc ggggaaggac
      661 cttgccgtgg cgctactctc ggtggagacg ggacgtcttg aggcgggcgt cttcccgaag
      721 ggggaggccg ccgcgctgct tggttccttc gctccggggg agctgctgca tcccgcgagc
      781 ctttcgctct ccgagtggtt cccgcttgcg ggcgactact ttcctctccc gagggaggac
      841 gagttcttca gcccggtcca gggaacgcgg cgtctgctgc aacagtggaa gacggcgacg
      901 ctggcctcct tcggcctgga ggacggctct cccgctgccg gagccgccgc cgccgtactg
      961 gcgtatctcc acgagacgca gttcggcgcg gtggaataca taaagcgggc gcacccgttg
     1021 cgctcgtcgg agcatctgca tctggacgtg acgacggggc ggaatcttga gcttttcgat
     1081 acgcgccagg agcagaacgg agacgctccg tccctctacc ggacgctgaa ccgctgccgt
     1141 tcgaccatgg ggagacggct gctcagggag tggctgctcc gtccgctgct cgacctcggc
     1201 gagctgcgcc gcaggcagga t
//
LOCUS       DAOSWW010000942         1221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_107029_length_1221_cov_2.204974, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000942 DAOSWW010000000
VERSION     DAOSWW010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107029_length_1221_cov_2.204974"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1221
                     /locus_tag="PK442_14440"
     CDS             <1..>1221
                     /locus_tag="PK442_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88901.1"
                     /translation="CLVIRGGKPLTDSSGTPYVGFELLVDARRAGRDSTKRYKEAVAK
                     RSAMEVENHHCPAGVKHVVDVRKFFAMEKAPFFDPPRPRSAAGKDAGIGSKSSVDEIV
                     RAFHNSPQCEAANSSLMGRRDALAREWNRFVAKHRGKAPEDRLERARHLDYTMRTAIY
                     EGHLDRGCSAYGACERNVIALSIRNRGRIACQRSQGCAFPGDFQGVSSKVSQYNIWDE
                     YLTQISGLTSCFLRDDLQRKPAGGEAPYNASYYIRIGRMYEQNAGDVEKILFGSDGDL
                     ASVFPRTPRADLVSLRHYYHPPAMRKCFPNHPRVEYMSGAVARNGKDYALIANTRIEV
                     GRKTGGDYLFKIFDFTQEGGDDVVALRNLYPGFSVDGRKVSLRISRGCVPYGIPSGCR
                     HKKIGRYRTVPGWL"
BASE COUNT          264 a          378 c          377 g          202 t
ORIGIN      
        1 gtgcctcgtc attcgaggcg gaaagcctct cacggactcc tccggcacgc cctatgtggg
       61 ctttgaactg ctcgtcgacg cgaggcgcgc cggtcgcgat tccaccaaga ggtacaagga
      121 ggcggtcgca aagagaagcg ccatggaggt cgaaaaccac cattgcccgg ccggagtgaa
      181 gcatgtggtc gacgtgagga agttcttcgc catggagaag gcccctttct tcgacccccc
      241 ccgtccgcgt tccgccgcag ggaaggatgc cgggatcggt tcgaaaagct ccgtggacga
      301 aatagtgcga gcatttcaca attcccctca gtgcgaagcg gcgaactcgt cgctcatggg
      361 caggcgcgac gcgctcgccc gcgaatggaa caggttcgtc gcgaagcacc ggggaaaagc
      421 ccccgaagac aggcttgaga gggcaaggca tctggactac acgatgcgca cggccatcta
      481 cgaggggcac ctggaccgcg gctgcagcgc ctacggggcc tgcgagagaa acgttatcgc
      541 actctcgata agaaaccgcg gccggatcgc ctgccagcgc tcacagggct gtgctttccc
      601 cggggacttt cagggcgtat cctccaaggt atcgcagtac aacatatggg atgaatatct
      661 gacgcagata tccgggctca cctcgtgctt cctgagggac gatctacaga ggaagcccgc
      721 gggaggggag gcgccctaca acgcctccta ctacatcagg ataggccgga tgtacgaaca
      781 aaacgcggga gacgtggaga aaatcctctt cggcagcgac ggcgacctcg cgtccgtctt
      841 tccgcgcacg ccgcgagccg acctcgtctc cttgcgccat tactaccatc cccctgcgat
      901 gaggaagtgc ttcccgaatc acccgcgcgt cgagtacatg tcgggcgccg tggcacgaaa
      961 cggaaaggat tacgccctca tagccaacac gcgcatcgag gtcggcagga agacgggggg
     1021 cgactacctc ttcaagatct tcgatttcac ccaggaggga ggagacgacg tcgtagctct
     1081 cagaaacctc taccccggat tctcggtgga cggcaggaag gtatcgcttc gaatctccag
     1141 ggggtgcgtg ccctacggca taccgtccgg ctgcaggcac aagaagatcg gccgctaccg
     1201 caccgtgccc ggatggctga a
//
LOCUS       DAOSWW010000943         1220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_107107_length_1220_cov_3.747639, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000943 DAOSWW010000000
VERSION     DAOSWW010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107107_length_1220_cov_3.747639"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1220
                     /locus_tag="PK442_14445"
     CDS             <1..>1220
                     /locus_tag="PK442_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOO88902.1"
                     /translation="ETEALGNPTEGALLLWLDERGEDYKALRDPFGVLDQLTFSTERK
                     YMATFGKGRDGGAVLHAKGAPEILLETCSSILSESGEEALTDGVKEEILSSIGSFQAR
                     GMRALGFAVRRELPEAGEVSAESLMEGLTWPGFTAIADPVRDDVPDAIRACTGAGIGV
                     KIVTGDNRETAREIGRQIGLVGDGPEAEFALLTGDEFETMVDAPETRLRLLDELKVLS
                     RARPSHKLKLVKALQEKGEVVAVTGDGTNDAPALNHANVGLSMGKRGTAVAKEASDIV
                     LLDDSFTSIVNAILWGRALYQNIQRFLLFQLTVNVTALLIVLLGPVVGIELPLTVIQM
                     LWVNLIMDTFAALALATEPPHSEVMKSKPRNPEAFIVTPGMAKGIFSYGVFFVLILAS
                     MLLLDETKCFAFCAG"
BASE COUNT          221 a          362 c          435 g          202 t
ORIGIN      
        1 gagacggaag cgctggggaa tcccacggag ggggcgctgc tgctctggct cgacgagcgc
       61 ggagaggact acaaggcgct gcgggacccg ttcggcgtgc tggatcagct cacattctcc
      121 accgagcgga agtacatggc gaccttcgga aaaggacgcg acggcggcgc ggtgcttcac
      181 gcgaaggggg cgcccgaaat actgctcgaa acgtgctcct cgatcctctc cgagtctgga
      241 gaagaggcgc tgacggacgg ggtgaaggag gaaattttgt cctccatagg ctccttccag
      301 gccaggggga tgcgcgccct cggcttcgcc gtgcgccgcg agcttcccga ggccggagag
      361 gtctctgccg agagccttat ggaggggttg acatggcccg gattcacagc tatcgccgac
      421 ccggtgcgcg acgacgtacc cgacgccatc cgcgcctgca cgggggcggg catcggcgtg
      481 aagatcgtca ccggcgacaa ccgggagacg gcgcgcgaga tcggccgtca gatagggctg
      541 gtcggcgacg ggccggaggc ggagttcgcg ctgctgacgg gggacgagtt cgagacgatg
      601 gtcgacgctc cggagacacg gctgcggctg ctcgacgaat tgaaggtgct ctcgcgggcg
      661 cgcccctcgc acaaactgaa gctggtgaag gcgctccagg agaagggaga agtcgtcgcg
      721 gtgacgggcg acgggacgaa cgacgcccct gcgctgaacc acgcgaacgt cgggctctcc
      781 atggggaagc ggggaaccgc cgtggcgaag gaggccagcg acatcgtgct gctcgacgac
      841 tcgttcacga gcatcgtcaa cgcgattctg tgggggaggg cgctctacca gaacatccag
      901 cgcttcctgc tcttccagct tacggtgaac gtgacggcgc tgttgatcgt gctgctcggc
      961 ccggtggtcg ggatagagct tcccctgacg gtgatccaga tgctctgggt caacctgatc
     1021 atggacacct tcgcggcgct cgccctggcg acggagccgc cgcacagcga ggtgatgaag
     1081 agcaaacccc ggaatccgga ggccttcatc gtgacgccgg ggatggcgaa ggggatcttc
     1141 agctacggcg tctttttcgt actcatcctc gcctccatgc tgctgcttga tgagacaaag
     1201 tgttttgcgt tctgcgcagg
//
LOCUS       DAOSWW010000944         1220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_107192_length_1220_cov_2.181116, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000944 DAOSWW010000000
VERSION     DAOSWW010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107192_length_1220_cov_2.181116"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PK442_14450"
     CDS             <1..719
                     /locus_tag="PK442_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOO88903.1"
                     /translation="GPTGSGNSTTLYSVLEQINKPEVNIVTVEDPVEYTLFGIAQVQV
                     NERAGLTFNSTLRSILRQDPDKIMVGEIRDGETAQLAIRAALTGHLVLSTLHTNDAPS
                     ASIRLIDMGIAPFLVASSLLAVIAQRLVRTLCPSCKEEYIIPEPVCKTLGLPIDTRGY
                     RPVGCNDCRGTGYKGRSAIFEIMLLNESIRELIVSGASADVIRKEAIRLGMTTLRESG
                     VRKIAAGVSSVEDVLTTTFS"
     gene            739..>1220
                     /locus_tag="PK442_14455"
     CDS             739..>1220
                     /locus_tag="PK442_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO88904.1"
                     /translation="MRIDELLAEVSVRGASDLHISEGLPPSFRVDGELRPLADFPSLS
                     REDMEEALQHLLSPEEMEKFRKSREHDFSFSFRSPDGEEARFRGNAYFESGRMGLALR
                     LIPMRIRTLDELGLPSTLKEISTHRRGLFLVTGPTGHGKSSTLAALLQMINTTRREHI
                     "
BASE COUNT          277 a          386 c          330 g          227 t
ORIGIN      
        1 ccggtcccac gggaagcggt aactcaacta ctctttactc tgttctcgaa cagatcaaca
       61 agccggaggt aaacatcgtc acagtagagg acccggtgga gtacactctc tttggtatcg
      121 ctcaggtgca ggtcaacgaa cgggcggggc ttactttcaa ctccacactc agatcaattc
      181 ttcgacagga ccccgacaag atcatggtcg gagaaatccg cgacggcgaa acggcccagc
      241 ttgccatacg cgcagccctc accggacacc ttgtcctctc caccctgcac accaacgacg
      301 ctccaagcgc gtcgatccgt ctcatagaca tggggatcgc ccccttcctc gtcgcctcct
      361 ccctgctcgc cgtcatcgct cagcgtcttg tgcgcacact ctgcccctcg tgcaaagagg
      421 aatacattat tccagagccg gtctgcaaaa cactcggcct ccccatcgat acacgcgggt
      481 accgtcccgt ggggtgcaac gactgccgcg gaacgggcta caaagggcgg agcgcgattt
      541 tcgagattat gctgctcaat gaatcgatac gggaactcat tgtcagcggc gcaagcgccg
      601 acgtcatacg gaaggaagcg atacgcctgg gcatgacaac gctgagagag tcgggagtac
      661 ggaagatagc cgccggtgtt tcgagtgtcg aagacgtgtt gaccacgacg ttctcatgac
      721 ggaaggagag gatacctcgt gcggatagac gaactgctgg ccgaagtttc ggtacgcgga
      781 gcaagcgatc ttcacatcag cgaaggactt ccgccaagct tccgcgtaga cggagagctt
      841 cgtcccctcg ccgatttccc ctcgctctcc agggaagaca tggaggaagc gctgcaacac
      901 ctgctttccc ccgaagagat ggagaaattc cgaaagagcc gcgagcacga cttcagcttc
      961 tcattccggt cgcctgatgg agaagaggcc cgttttcgcg gcaacgccta tttcgagtcc
     1021 ggcagaatgg ggctggcgct ccggctcatc ccgatgcgta tccgtacgct ggacgaactc
     1081 ggactgccgt cgacgctcaa agaaatttcc acgcatcgca gagggctctt cctggtaacc
     1141 ggccccaccg gccacggaaa aagctccacg ctcgccgcgc tcctccagat gatcaatacc
     1201 acccgcaggg agcacatcat
//
LOCUS       DAOSWW010000945         1218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_107451_length_1218_cov_2.828891, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000945 DAOSWW010000000
VERSION     DAOSWW010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107451_length_1218_cov_2.828891"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PK442_14460"
     CDS             complement(<1..426)
                     /locus_tag="PK442_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88905.1"
                     /translation="MKLLFERWRDNPQVQEATEKFSAAAGRFLSVRGSSETDDRIAAR
                     KYWSELSSMYWTVAVALIDAQTESFPEELVFDEQERLFLDLGVVDQELTPFHPEITRL
                     LNSRAPAGLFQYYSFSDHIAECYSMVMAKPVTPPRSGYSI"
     gene            577..1056
                     /locus_tag="PK442_14465"
     CDS             577..1056
                     /locus_tag="PK442_14465"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOO88906.1"
                     /translation="MGHPMRRNDREITDPAEIDGILEKALFGHLGVCDGDEPYVLPVN
                     FGVSEGAIYFHCAPEGRKLDVLRRNPKACFQVETDTELVASTTACGWGMLYRSVVVSG
                     VVSVVDSLEEKARGLTSLMKKCAGDSFSHVFSEQELGAVVVLRLDAVSRTGKARRPA"
BASE COUNT          243 a          347 c          382 g          246 t
ORIGIN      
        1 gatggagtag ccgcttcgcg gcggggtgac aggtttggcc atgaccatgc tgtagcactc
       61 cgcaatgtgg tcggagaacg agtagtactg aaagagcccc gcgggcgccc ttgagttcag
      121 caggcgggtg atctcgggat gaaagggggt gagctcctgg tccacaacgc caagatcgag
      181 aaagagccgt tcctgctcgt caaagacaag ttcttcgggg aatgattccg tctgtgcgtc
      241 gatgagagcg acagccaccg tccagtacat cgaggaaagt tccgaccagt attttctggc
      301 ggcgatccgg tcgtccgtct cggaggagcc gcgcacggac aaaaaacggc cggcggcggc
      361 agaaaacttc tccgtggcct cctgtacctg cgggttgtcc ctccatcgtt caaacagtag
      421 cttcattcgg tatcatcctc cgattctccg tgcgtgtacc ggacttcctt cacgttgcat
      481 gcgattctga cgtgcggaat accgtgttta tgataccata ttccacagcg cacatattca
      541 tccggaacag atgatacttc aaaaggagga aaaaccatgg gacatccgat gcggcgaaac
      601 gacagggaaa tcaccgatcc tgcggagatc gacggcattt tggagaaggc ccttttcggg
      661 catcttgggg tgtgcgacgg cgacgaaccg tacgtccttc cggtcaactt cggcgtctcc
      721 gaaggggcga tctacttcca ctgcgccccg gaagggcgga agctcgacgt gctgcggcgc
      781 aacccgaagg cttgttttca ggtggaaacc gataccgaac tcgtcgcctc caccacggca
      841 tgcggttggg gcatgctcta ccgaagcgtc gtggtttccg gcgtcgtctc cgtcgtcgac
      901 tctctcgaag agaaggcgcg aggcttgacc tcgctgatga agaagtgcgc gggagactcc
      961 ttctcccatg tgttttcgga gcaggagctt ggggctgtcg tcgtgctccg gctcgatgcg
     1021 gtctcccgga cggggaaagc ccgccgtccg gcctgatacg gggcgacggg aggatgttcc
     1081 gcatcaggag ggttttcgac gcacgctccc cggcgaacgt aagggcgatt gcgcgtgttc
     1141 aggagatctt gcgggagcag ttccccgaag cccgtcccgg cgaggcggac gcgatccccg
     1201 agaagctctc gaatcacc
//
LOCUS       DAOSWW010000946         1217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_107643_length_1217_cov_2.237522, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000946 DAOSWW010000000
VERSION     DAOSWW010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107643_length_1217_cov_2.237522"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1217)
                     /locus_tag="PK442_14470"
     CDS             complement(<1..>1217)
                     /locus_tag="PK442_14470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO88907.1"
                     /translation="DGSILWAICAGRVVEWAEDGSPVTMVGCYIDITERKRAEEEMHL
                     LATRQHAMEVAKESAEAANKAKSAFLANMSHEIRTPLNAILGFGAALERDPSLSEKQA
                     EQVRTINRSGRHLLRLLNDILDMSRIEAGRLTLTQAPFCLHDLLDDLESMFRLRAESR
                     GLHLDMEYGEDVPRYAVGDEAKLRQVLLNLLSNAVKFTKEGGVAMRVHADALNPGKEF
                     VSLLIDVEDTGPGIDLIELERIFEPFRQSEAGREAGGAGLGLAISKQLAELMGGRLTV
                     ESRQGDGSRFSVLLPLRRSEEGAGPKQDPRRVVGVENGGKEYRILVVDDKKENRDVLR
                     ALLEPMGFALKESANGTEALKVFGEWSPHAILMDMRMPVMDGYEATRRIKADVGGAAM
                     PIVAVTSFAFEED"
BASE COUNT          204 a          411 c          351 g          251 t
ORIGIN      
        1 cgtcctcctc gaaagcgaag gacgtgacgg cgacgatggg catcgccgcg cccccgacgt
       61 ccgctttgat tcgacgggta gcctcgtacc cgtccataac cggcatgcgc atatccatca
      121 gaatcgcgtg cggcgaccac tcgccgaaaa ctttcagcgc ctccgtaccg ttcgcgctct
      181 ccttcagcgc gaatcccatg ggttccagca gcgcccgcag cacgtcgcga ttctcctttt
      241 tatcgtccac cacgagtatc cggtactctt ttccgccatt ctccacgccg acgacgcgcc
      301 ggggatcctg tttgggaccg gcgccctcct cgctgcggcg gagcggcagc agaacggaaa
      361 agcgacttcc gtcgccctgc ctgctctcga ccgtaaggcg cccgcccata agctcggcga
      421 gctgtttgct gatggcgagg ccgagtccgg cgcctccggc ctctcttccg gcttccgact
      481 gacggaaggg ttcgaagatc ctctccagtt cgatcaggtc gatcccgggg ccggtgtctt
      541 cgacgtcgat cagcagactc acgaactctt tgccggggtt cagcgcatcc gcgtggacgc
      601 gcatcgcgac gcccccttct tttgtgaact tcaccgcgtt ggacagaaga ttgagcagta
      661 cctgacgcag cttcgcctcg tcccccacag cgtagcgcgg cacgtcttcg ccgtactcca
      721 tgtccaggtg aaggccccgc gattcggcgc gcagcctgaa catcgattcc agatcgtcca
      781 gcagatcgtg gagacaaaaa ggagcttgcg tcagcgtcag ccgtcccgcc tcgatccggg
      841 acatatccaa aatgtcgttg agaagacgca gcagatgccg tccgctcctg ttgatggtgc
      901 gcacctgctc ggcctgtttc tcggagagtg agggatcgcg ttcgagcgcc gcgccgaagc
      961 cgagaatggc gttgagcggc gtgcggattt cgtggctcat gttcgcaagg aaggcgctct
     1021 tcgctttatt cgcggcctcc gcgctctcct tggcgacttc catggcgtgt tgacgcgtgg
     1081 cgagaaggtg catttcctcc tccgcccgtt tgcgttcggt gatgtcgatg taacatccca
     1141 ccattgtgac gggagagccg tcttcggccc attcgaccac ccgacctgcg caaatcgccc
     1201 agaggatcga gccgtcc
//
LOCUS       DAOSWW010000947         1213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_108155_length_1213_cov_4.256477, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000947 DAOSWW010000000
VERSION     DAOSWW010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108155_length_1213_cov_4.256477"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PK442_14475"
     CDS             complement(<1..1074)
                     /locus_tag="PK442_14475"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOO88908.1"
                     /translation="MNTKKTGSAKAATETIALESINVLTPGSAPVALKLARIGGIGET
                     VDGSVSWDRSRCSLSPGIVAETLVSALLCGCRALYNMKSFWEESGCAAWFERNGVVPE
                     QLNDDVYARLLDRFSECDMRKIVETVAFRLRTAHGIKVSTVHFDTTSVSVEGDYATTK
                     EERGEESDGAETSEPAAADAEKPSFEIERGHSKDRRPDLKQIKIGLAVQEDGIPLTGE
                     LLSGSASDRTWNGDAVGYISELLGGQGVEDAVFVADSALVSKENLAELADGGMDFVTL
                     LPGTFSLEKELKERAWREEKWTDLGALARDPDEKSARYKTRSVREEIEGRTYDFLVVH
                     STKLEAGVCQPTCRFSGVKVSRAL"
BASE COUNT          222 a          400 c          325 g          266 t
ORIGIN      
        1 aagagcgcgg gaaactttaa ccccggaaaa gcgacaggtc ggttgacaga caccggcttc
       61 gagcttcgtc gagtggacca cgagaaagtc gtacgttctt ccctcgatct cctcccggac
      121 gctccgcgtc ttgtagcgcg cgctcttttc gtcgggatcc cgggcgagag cgccgaggtc
      181 cgtccacttc tcttcgcgcc acgctctctc cttgagctcc ttctccagag aaaatgtccc
      241 gggaagcagc gtcacgaaat ccattcctcc gtcggcgagc tccgccaggt tctccttgct
      301 cacgagcgcg ctgtcggcca cgaaaacggc gtcttcgact ccctgccctc cgagcagttc
      361 cgatatgtac ccgacggcgt ctccgttcca cgtccggtcc gaagcgctgc cggagagaag
      421 ttctcccgtg agcggaatcc catcctcctg caccgcgagg ccgatcttga tctgcttcag
      481 gtccggacga cggtccttgg agtgcccgcg ctcgatctcg aaagagggct tttcggcgtc
      541 cgcggcggcg ggttcggacg tctccgcgcc gtcgctctcc tctccccgct cttctttcgt
      601 cgtcgcatag tcgccttcca cggagaccga cgtcgtgtcg aaatgcacgg tcgaaacctt
      661 gatcccgtgc gccgtcctga gcctgaacgc cacggtctcc actattttgc gcatatcgca
      721 ttcggagaac ctgtccagaa gccgcgcgta cacatcgtcg ttcagctgct ccggcacgac
      781 gccgttcctc tcgaaccagg cggcgcatcc ggactcctcc cagaaggact tcatgttgta
      841 gagcgcccga cagccgcaaa gaagagccga tacgagcgtt tctgccacta ttccgggaga
      901 gagggagcat cgggaacggt cccacgacac gctcccgtcc accgtttctc cgattcctcc
      961 tatgcgcgcg agtttcagag cgacgggagc ggagccgggg gtgagcacat ttatggattc
     1021 aagcgcgatt gtctccgttg cagcttttgc gctgccagtc ttttttgtat tcatacgacc
     1081 tattttatgg caaatacgac acattgtcac ctgcctcaaa aaatacccga tcattcaaat
     1141 cgacatgcga aatacggggt agaggatttc catatcgaat ccttttgccc ggcgtgcgac
     1201 cgcctggccg att
//
LOCUS       DAOSWW010000948         1213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_108171_length_1213_cov_3.317789, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000948 DAOSWW010000000
VERSION     DAOSWW010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108171_length_1213_cov_3.317789"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1213)
                     /locus_tag="PK442_14480"
     CDS             complement(<1..>1213)
                     /locus_tag="PK442_14480"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOO88909.1"
                     /translation="DITIYLFAAFLGVFASRMTPDPFLYGVSVVYFAMALVTAVHTLA
                     FEGMGVFPGWTANHPTQLWMLMRFIHGIGVPLTLLFSSRPLFIRFFTAGAMLSVFGGI
                     LLIAAGYFPDCFIPGEGLTLFKVAGEYAVALLLVLSILYSLAYRRREARRYGYSFEIS
                     LACFIASGMAFTLYTDVYGFFNMLGHILHGYGAYILLFGFVVKSAQTLMDRHFYDLNE
                     QIRNMNRELENRVAQRTRELEEANALLASDIARRKEAEEHLRRAKEEAEEANRAKSAF
                     LATMSHEVRTPLNGILGMAAHLKDEDLKPEEREEYLSILVKSGESLLDILNNILDIAR
                     LESGSAREERSSFRPAVLAQEVVALFAHRAAEKGIALTPFVDPNLPEVLLGYPMRLKQ
                     VLFNLVGNGIKF"
BASE COUNT          248 a          355 c          392 g          218 t
ORIGIN      
        1 tgaacttgat tccgttgccg accaggttga agagcacctg cttcaggcgc atcgggtagc
       61 cgagcaggac ttccggcaga ttcgggtcga cgaatggcgt cagagcaatc cccttttccg
      121 ccgcccggtg tgcgaagagt gcgacgacct cctgggcgag aacggcgggc cggaaagagg
      181 atcgttcctc ccgcgcgctt cccgactcca gccgcgcgat gtcgaggatg ttgttcagga
      241 tgtccagcag cgactccccc gatttgacga ggatggagag gtactcttcc cgctcttccg
      301 gcttcagatc ctcgtccttc aggtgggccg ccatgccgag aatgccgttg agcggcgtgc
      361 ggacttcgtg gctcatcgtg gcgaggaagg cgctcttcgc ccggtttgcc tcctccgctt
      421 cctccttggc cctgcggaga tgctcctcgg cctccttgcg ccgggcgata tccgaggcaa
      481 gaagcgcgtt tgcctcctcc agctcgcgcg ttcgctgcgc gacacggttt tccagttcgc
      541 ggttcatatt ccgtatctgc tcgttcaagt cgtaaaaatg cctgtccatc agcgtttggg
      601 ccgacttgac gacgaagccg aagagcagaa tgtacgcgcc gtagccgtgg agaatatgcc
      661 ccagcatatt gaagaagccg tagacgtccg tgtagagggt gaaggccatt ccgcttgcga
      721 tgaagcaggc gagcgaaatt tcgaaggagt agccgtatcg ccgcgcctcc ctgcggcggt
      781 acgcgagcga atacaggatg gaaagaacaa gcagcagcgc gacggcgtac tcgcccgcca
      841 ccttgaagag cgtgagtcct tcgccgggga tgaagcagtc cgggaagtat cccgccgcga
      901 tcagcagaat gccgccgaag accgagagca tcgcgcccgc ggtgaaaaaa cggatgaaca
      961 gaggacgcga ggagaagagg agcgtcagcg gaacgccgat tccgtgaatg aagcgcatca
     1021 gcatccagag ctgcgtgggg tggttcgccg tccaccccgg gaagactccc attccctcga
     1081 aggcgagcgt gtggacggcc gtgacgagcg ccatggcgaa gtagacgacg ctgacgccgt
     1141 agaggaaagg atccggcgtc attcgcgagg cgaagactcc gagaaaggcc gcgaagagat
     1201 agatcgtgat gtc
//
LOCUS       DAOSWW010000949         1213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_108176_length_1213_cov_3.120035, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000949 DAOSWW010000000
VERSION     DAOSWW010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108176_length_1213_cov_3.120035"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PK442_14485"
     CDS             <1..723
                     /locus_tag="PK442_14485"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO88910.1"
                     /translation="QWRDGPTEIVRKDRRKAVTVEADARYITVGEATENARRAIEGIE
                     LPPGYEIQFGGDAEFMAENFAELGQALLIAIVLTFIIVAAILESWFFAFVITLSVPLA
                     ALGVIPAMLMSGVSISLFALIGLIMLVGLVVNNAIVVIDYAEVRRVEEGEPGSIAIEK
                     ACAIRLRTLFMAISTAVISMVPLAAGTGDGGSFRQPIAFVAIGGLIAGGIIALLVIPA
                     IYSLYWGFKERRAARRAAAGQL"
     gene            957..>1213
                     /locus_tag="PK442_14490"
     CDS             957..>1213
                     /locus_tag="PK442_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO88911.1"
                     /translation="MRILIASVVLVALLASGAFASDLNAYTIMPEKYASQVFEAFTAE
                     TGVKVNFMRFSSGEALARVVAEKNNPQVDMILGGPADTYEAG"
BASE COUNT          218 a          347 c          372 g          276 t
ORIGIN      
        1 cagtggcgcg acggtcccac ggagattgtc aggaaggacc gccggaaggc ggtgactgtg
       61 gaggccgatg cacgctatat cacggtgggc gaagcaacgg aaaacgcccg gcgtgcaata
      121 gagggtatcg aactgcctcc cgggtatgag atccagttcg gcggcgatgc ggagttcatg
      181 gcggagaact ttgctgaact cggccaggcc ctgttgatcg ccatcgtgct caccttcatc
      241 atcgtcgcgg cgatactgga gtcgtggttc ttcgccttcg tgatcacgct ctcggtaccg
      301 ctggccgcgc tcggcgtcat cccggcgatg ctgatgtcgg gagtttccat ttcgctcttc
      361 gcgctcatcg gcctgatcat gctcgtcggc ctggtggtga acaacgccat tgtcgtgatc
      421 gactacgccg aggtccgccg cgttgaggag ggcgagccgg gatcgatcgc gatcgaaaaa
      481 gcgtgcgcaa ttcgtctacg aaccctcttc atggccatct cgacggcggt gatctcgatg
      541 gtgcctcttg cagcgggcac gggagacggc ggttcgttcc gccagcccat cgccttcgtc
      601 gccatcggcg gtttgatcgc cggcggtatc atcgcgctgc tggttattcc ggcgatttat
      661 tcgctctact ggggtttcaa ggagcgcaga gcggctcgcc gcgcggcggc cgggcagctg
      721 taagaaagcg cgattcaatc agtttttcta tgaagcgggg gccggaagat atcggtcctc
      781 gcttttctta atttttaacg ggatccggcg gacgttcagt gttccctctc ttcgtccccg
      841 cgttcatcgt tttttgtatt gcagaatgag cgtcttcacg agataataag cgtgatgttg
      901 cactaccgtt acacatcgtt cacatacctc aaaaggagat gattctgttg tcgaaaatga
      961 gaatcctcat cgcgtccgtc gtattggttg ctctcctggc gtccggggcc ttcgcctcgg
     1021 atttgaacgc gtacaccatc atgcccgaga agtacgcgtc ccaggtcttc gaggccttca
     1081 ccgccgagac cggcgtcaag gtgaacttca tgcggttctc gtccggagag gcgctggcca
     1141 gagtggtggc ggagaagaac aatccgcagg tcgacatgat cctcggaggc cccgccgaca
     1201 cctacgaagc cgg
//
LOCUS       DAOSWW010000950         1212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_108333_length_1212_cov_2.881590, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000950 DAOSWW010000000
VERSION     DAOSWW010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108333_length_1212_cov_2.881590"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1212
                     /locus_tag="PK442_14495"
     CDS             <1..>1212
                     /locus_tag="PK442_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HOO88912.1"
                     /translation="YRGKIPAPSRREYLPDERDAILITYGDQFRAPGEPPLRTLRGFL
                     DTHLEGCVSGVHILPCFPYTSDDGFSVVDYRRVDPELGDWNDIAALGERYRLMLDFVA
                     NHISRRSDWFASFLRGEAPYTDYFITPPEDADISRVVRPRTLPLLTKVETAQGERSVW
                     TTFSADQIDLNYAAPRVLLEMTGILLHYAFRGAEIIRLDAIAYLWKTPGTSCIHLPKT
                     HAVVRFWRAVLDDVAPHVLLITETNVPHAENVSYFGEAREDGRGTDEAQMVYNFSLAP
                     LTLHALLTGDATVLSTWASELSPPARGATFFNFIASHDGIGVTPARGILSDEQVRRLA
                     ERALAHGGHVSYKTNADGSISPYELNITLYDFLNDPAKPEPGTDAARFIASQAILLSL
                     AGVPGISVHSL"
BASE COUNT          231 a          428 c          356 g          197 t
ORIGIN      
        1 cgtaccgagg aaaaataccg gccccctcgc gaagagagta tctaccggac gaacgggacg
       61 ccatcctgat cacctacggc gaccagttcc gcgcgcccgg agagcccccc ctccgaacgc
      121 tgcggggctt cctggacaca cacctcgaag ggtgcgtcag cggcgtgcac atcctcccct
      181 gcttccccta cacctccgac gacggttttt cagtcgtcga ctaccggcgc gtggacccgg
      241 agctgggtga ctggaacgac atcgccgcgc tgggagagcg ataccgcctg atgctggact
      301 tcgtcgccaa ccacatctca cgacggagcg actggttcgc ctcctttctc cgcggggagg
      361 cgccctacac ggactatttc atcacccccc ccgaagacgc cgacatctca cgggtggtgc
      421 ggccgcgcac gctgccgctg ctgacaaagg ttgagacggc ccaaggcgaa cggagcgtct
      481 ggacgacctt cagcgccgac cagatcgatc tgaactacgc cgcgccgcgc gtgctgctgg
      541 agatgaccgg catcctgctc cactacgcat tcaggggcgc ggagatcata cggctggacg
      601 ccatcgccta tctctggaag acgccgggaa catcctgcat ccatctgccg aaaacgcatg
      661 cagtggtaag attctggcgc gccgtgctcg acgacgtcgc cccgcacgtg ctgctgatca
      721 ccgagaccaa cgtccctcac gccgaaaacg tcagctactt cggcgaggcg cgggaagacg
      781 ggcgaggaac ggacgaagcg cagatggtgt acaacttttc gctggcgccg ctgacgcttc
      841 acgccctgct cacaggagac gcaaccgtcc tctcgacgtg ggcctcggag ctttcccccc
      901 cggcacgcgg agcgaccttc ttcaacttta tcgcctcgca cgacggcatc ggagtgacgc
      961 cggccagggg catcctgagc gacgaacagg tgcggagact cgcggagcgc gcgctcgctc
     1021 acggcgggca cgtctcgtac aagacgaacg ccgacggctc catcagtccg tacgagctga
     1081 acatcaccct ctacgacttc ctgaacgatc cggcgaaacc ggagccgggg acggacgcag
     1141 cgcgttttat cgcctctcag gcaatcctgc tctcgcttgc cggagttccg ggcatctccg
     1201 tccacagcct ct
//
LOCUS       DAOSWW010000951         1211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_108486_length_1211_cov_3.042388, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000951 DAOSWW010000000
VERSION     DAOSWW010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108486_length_1211_cov_3.042388"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PK442_14500"
     CDS             <1..798
                     /locus_tag="PK442_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO88913.1"
                     /translation="PHFFCPGCGCGQVVSAFLRACESLELDLGTMVGVGGVGCTARIP
                     VYINMDCLHGVHGRTLAWATGIKLHKPETKVVVFAGDGDALSIGGNHFIHAARRNLDV
                     TFVVVNNFNFAMTGGQVAPMTPDGAVTMTTPYGSSEPPFDLCKLAVTAGATYVARTLT
                     SSPPQMTTFLKNALSHKGFSVVEILSQCPTHFGRYALGSGSPKNLVEWIKNHSVPVSA
                     AEKMKPEEIAGKYLIGEFVKTERPVFRGSTVYTAPRQESAERKEAKA"
     gene            795..>1211
                     /locus_tag="PK442_14505"
     CDS             795..>1211
                     /locus_tag="PK442_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO88914.1"
                     /translation="MSRTSIRFCGFGGQGIILSSVILGEAAVTKEGIYAAQSQSYGSE
                     ARGGQCQSEVVLSKNPIGTPTNEQNDILVAMFQAAYMSHKHELKKGGVLFVDSGLVPD
                     LGDVDPSVTVWKVPATETAIRLGSKMAGNMVMLIYAI"
BASE COUNT          234 a          399 c          373 g          205 t
ORIGIN      
        1 ccccatttct tctgccccgg ctgcggctgc ggtcaggtgg tcagcgcctt tctccgtgcg
       61 tgcgagtcgc tcgaactgga cctcgggacg atggtcggcg tcggcggcgt cggctgcacg
      121 gcgcgcattc cggtatacat caacatggac tgcctccacg gcgtccacgg cagaacgctt
      181 gcctgggcca ccggcatcaa gctgcacaag ccggagacca aagtcgtcgt gttcgccgga
      241 gacggcgacg ccctctccat cggcggcaac cacttcatcc acgcggcgcg ccggaatctc
      301 gacgtcacct tcgtcgtggt gaacaacttc aacttcgcga tgacgggcgg gcaggtcgcc
      361 cccatgacgc ccgacggcgc cgtgaccatg accaccccct acggaagcag cgaacctccg
      421 ttcgacctct gcaagctcgc ggtcaccgcc ggagcgacgt atgtcgcccg tacgctgacc
      481 tccagtccgc cgcagatgac gacgttcctg aagaacgcgc tctcccacaa ggggttctcc
      541 gtcgtggaga ttctctccca gtgtccgacg cacttcggca ggtacgcgct cggcagcgga
      601 agcccgaaga acctcgtcga gtggatcaag aatcacagcg tacccgtatc cgcagcggaa
      661 aagatgaagc ccgaagagat cgccggaaag tatctcatcg gcgagttcgt gaagacggag
      721 cgtccggtct tccgggggag tacggtctat acggctccgc gccaggagag tgcggagcgc
      781 aaggaggcga aagcatgagc cggacgagca tccgtttttg cggtttcggc ggccagggga
      841 tcatcctctc ttcggtgatt ctcggagaag cggccgttac gaaggagggc atctacgcgg
      901 cccagtccca gtcctacggc tccgaggccc ggggcggcca gtgccagtcc gaggtggtcc
      961 tgtcgaagaa ccccatcggc acccccacca acgagcagaa cgatatcctc gtggccatgt
     1021 tccaggcggc ctacatgagc cacaaacatg aactgaagaa gggcggcgtc cttttcgtgg
     1081 acagcggcct cgtgcccgac ctcggcgacg tggatccctc agtcaccgtg tggaaggttc
     1141 ccgccacaga gacggccatc cgtctcggca gcaagatggc gggcaacatg gtcatgctga
     1201 tatacgccat a
//
LOCUS       DAOSWW010000952         1210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_108622_length_1210_cov_4.485714, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000952 DAOSWW010000000
VERSION     DAOSWW010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108622_length_1210_cov_4.485714"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK442_14510"
     CDS             <1..240
                     /locus_tag="PK442_14510"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOO88915.1"
                     /translation="EKGVPALYSSVFLDHQADETFMETQFLRALALAKRRGWVVVIAH
                     TRIATLQYLKKLEGALPDGYIFVSLPDLFHLLRIR"
     gene            313..>1210
                     /locus_tag="PK442_14515"
     CDS             313..>1210
                     /locus_tag="PK442_14515"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOO88916.1"
                     /translation="MKGRVEVIIGAQWGDEGKGRVVDALGAKVDIFARYQGGANAGHT
                     VIVEGQKYVFHLLPSGMLYPCKICIIGNGVVIDPEQLLKELKDLQDQGKDRARLVISG
                     AAHVVMPYHKVLDKAQEELRGKGRKIGTTGRGIGPAYVDKYSRCGIRVEDLLDGDVLR
                     EKLEYNLDEKNTYLSKIYGEEPLPFHEMYSIALSWGKSLAPYVGDASLIIDEALREGK
                     GVLFEGAQGTLLDIDHGTYPYVTSSSPVAAGGCVGLGVGPSMVGRVIGVVKAYCTRVG
                     EGPFPTEDCGDEGNKLREKGAEF"
BASE COUNT          263 a          302 c          397 g          248 t
ORIGIN      
        1 gaaaagggcg tgccggctct ctattcttct gtcttcctgg atcatcaggc ggacgagacg
       61 ttcatggaaa cgcagtttct ccgtgcgctc gctcttgcga aacggcgcgg gtgggttgtc
      121 gtcattgcgc acacgaggat cgcgacgctg caatatctga aaaagttgga aggagcgctt
      181 ccggacggct acatattcgt ctcccttccg gatttgttcc atctgctccg gatacgctga
      241 ttgcttgtga agcgagagga gcagcgcgga ttttttccgc aagaacgtgg cactacctga
      301 ggaggctgta ctgtgaaagg aagagtcgag gttattatcg gcgcccagtg gggcgatgaa
      361 ggaaaaggac gggttgttga cgcccttggt gcaaaggtcg atattttcgc ccgctaccag
      421 ggaggggcga atgcagggca tacagtaatc gtcgaggggc agaaatacgt cttccacctt
      481 ttgccgtcgg ggatgttgta cccgtgcaag atttgcatca tcggcaacgg ggtggtcatc
      541 gaccccgagc agcttctgaa agagctcaag gatcttcagg accaggggaa agaccgcgcc
      601 cggctggtga taagcggcgc ggcgcatgtc gtgatgccct atcacaaagt gctggacaag
      661 gcccaggaag agcttcgggg caaggggagg aaaatcggca cgaccgggag aggaataggc
      721 ccagcgtacg tcgacaagta cagccgctgc ggaatccggg tggaagatct cctggacggc
      781 gacgtcctca gagagaagct ggaatacaac cttgacgaga aaaatacgta cctctccaaa
      841 atatacggcg aggagcctct tccgtttcat gaaatgtact cgatcgcgct ctcatgggga
      901 aaatcgctcg cgccgtacgt cggcgacgcc tcgctgatca tcgacgaggc ccttcgggag
      961 ggcaaaggcg tgctttttga gggcgcgcag gggacgctgc tcgatattga tcacggaacc
     1021 tatccctatg tcacgagttc ttctcctgtt gcggcgggag gctgtgtcgg cctgggagtc
     1081 ggcccttcga tggtggggcg ggttattggc gttgtaaaag cctactgcac ccgggtcggc
     1141 gaggggcctt tccccacgga ggattgcggc gacgaaggaa acaagctgcg ggagaagggt
     1201 gcggaattcg
//
LOCUS       DAOSWW010000953         1208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_108902_length_1208_cov_4.896791, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000953 DAOSWW010000000
VERSION     DAOSWW010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108902_length_1208_cov_4.896791"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..755)
                     /locus_tag="PK442_14520"
     CDS             complement(141..755)
                     /locus_tag="PK442_14520"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOO88917.1"
                     /translation="MDLSILFRGVVIGFSIAAPVGPIGVLCIRRTLTEGRASGLVSGL
                     GAASADAVYGCIAGFGLTFLSNFLVSRQTELRLAGGLFLLYLAVKTFFAQPAQKAAEV
                     RGHGLLGAYLSTLFLTLTNPMTIISFAAIFAGLGLAEGSGNYISAGVLVLGVFAGSAL
                     WWLLLSGGFGLFREKFTASGLRWLNRLSGVIIGVFSLFALWSVF"
     gene            complement(887..>1208)
                     /locus_tag="PK442_14525"
     CDS             complement(887..>1208)
                     /locus_tag="PK442_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HOO88918.1"
                     /translation="ESAGVSLSSPAWLYGVTTVLSNLVSNVPAVMLLLPLVRDVPQAG
                     YILAVSSTFAGNLLIVGSIANIIVAGEAERMGIPFDWKQHALVGVPVTLISLAAGLAC
                     LWLF"
BASE COUNT          315 a          351 c          314 g          228 t
ORIGIN      
        1 agctgttcaa gactgttgct cgtatagagg tgtattccct tcatgcgtta tcccgaacaa
       61 cactcctttc tgaagtcggt acgaaacgcg cgctgcttct tttacgggcg cccccttttc
      121 atcatgaggg cattatgaat ctagaaaacg ctccacagtg cgaagagact aaaaacaccg
      181 atgattaccc cggaaagtct attgagccat ctgagaccgc tggcggtgaa tttctcccgg
      241 aacaggccga agccgccgct caaaagaagc caccagagag ctgaacccgc aaacaccccg
      301 aggacgagta ctccggcgga tatatagttt ccgctccctt cggccagtcc gagtccggca
      361 aatatggcgg cgaacgaaat aatcgtcatc ggattagtga gcgtcaggaa caaggtggag
      421 agatacgccc caagtaggcc gtgtccgcgt acctcggccg ctttttgtgc gggctgtgcg
      481 aaaaacgtct tgaccgccag atacagcagg aaaagacctc cggcaaggcg aagctccgtc
      541 tgcctgctga ccagaaagtt cgagagaaac gtcaagccga agcccgcgat acacccgtac
      601 accgcatccg cactcgccgc gcccagtcct gaaacaagac ctgacgcacg cccttcggtc
      661 agcgtgcgcc ggatacataa aactcctatg ggaccgacag gcgcggcgat cgaaaacccg
      721 ataacaacgc ctctgaacaa tatcgagaga tccatcttgc tcctccgttt ccagaatggg
      781 ggccttccgg gaagtcaccc cgtcaatata ccactttcct atcaaaagac aagggaataa
      841 agttgattct atggacaatt caacgagtat tcatgatatc gattttttaa aaaagccaga
      901 ggcaggcgag tccggcggca aggctgatca gggtcaccgg aactcccacg agcgcgtgct
      961 gcttccagtc gaatggaatc cccatacgct ccgcttcgcc tgcgacgatg atgttggcga
     1021 tgctccccac gatcagcaga tttcccgcga aggtgctcga caccgcaagg atgtaaccgg
     1081 cctgcggaac gtcgcgcaca agtgggagca gcagcatgac ggccggaaca ttggagacaa
     1141 gattcgagag cactgtcgtc actccgtaaa gccaggccgg agaggaaagc gatacccctg
     1201 ctgattca
//
LOCUS       DAOSWW010000954         1208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_108907_length_1208_cov_4.133565, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000954 DAOSWW010000000
VERSION     DAOSWW010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108907_length_1208_cov_4.133565"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK442_14530"
     CDS             <1..202
                     /locus_tag="PK442_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclophilin-like family protein"
                     /protein_id="HOO88919.1"
                     /translation="EIGEVAMWPPGNAFCIFFGPTPASVGAEPRAASPCVPLGRIKGD
                     ATLFRNFGSSLKNATLTAGNQV"
     gene            complement(328..>1208)
                     /locus_tag="PK442_14535"
     CDS             complement(328..>1208)
                     /locus_tag="PK442_14535"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOO88920.1"
                     /translation="HGSPANFMSKFYHFVSYGLNPQTEQIDYDELEALAKEHKPRLII
                     AGGSAYPRLIDYERIARIAHSAGGLLMVDMAHVAGLVAAKVIPSPVPHADFVTSSTTK
                     TLCGPRGGMVLCKAEHAKALDKGVFPGAISSIHLNIMAAKTWLFKHAASEEFKQVMTQ
                     IVANSRALAAELTGYGFRVISGGTDNHIVLLDLRPKKITGKLFQDALEYVGITINKNM
                     IPSDPEKPSVTSGVRIGTTSVSQRGLKEPEIKRIAAIMNEVAGAPEDKNVLDACKAEA
                     MRLISAFPLYPKGPLE"
BASE COUNT          189 a          388 c          392 g          239 t
ORIGIN      
        1 cgagattggc gaagtcgcga tgtggccgcc gggcaacgcc ttctgcatct tcttcggccc
       61 cacccccgcc agcgtcgggg ccgagccccg cgcagcctcg ccctgcgttc cgctcgggag
      121 gatcaagggg gacgcgacgc tcttccggaa cttcggttcg tcgctgaaga acgcgacgct
      181 caccgccgga aatcaggtct aaaaataaaa agcgcccgag agatccggca cgtttctgca
      241 gggatcactc aggcgcttcg ctgtcgcaga cccctcgttc gaagctaacg caatccgccg
      301 ggggaggata tacgggttct gtttccctta ctccagcggc cccttcgggt agagcgggaa
      361 agccgatatc agccgcatcg cctcggcctt gcaggcgtcg aggacgtttt tgtcctcggg
      421 ggccccggcg acctcgttca tgatcgcggc gatccgcttg atctccggct ccttcagccc
      481 gcgctgcgag acggaggtcg tcccgatgcg tacgccgctg gtgacggagg gcttctccgg
      541 gtcggagggg atcatgttct tgttgatcgt gatgcccacg tattcgaggg cgtcctggaa
      601 gagcttgccc gtgatcttct tcgggcgcag gtcgagcagg acgatgtggt tgtccgtccc
      661 cccgctgatc acgcggaagc cgtagccggt cagttccgcc gccagcgctc tcgaattcgc
      721 cactatctgc gtcatgacct gcttgaactc ttcggaggct gcgtgcttga agagccacgt
      781 cttcgccgcc atgatgttca gatggatgga gctgatcgcg ccggggaaga cccccttgtc
      841 cagcgccttc gcgtgctccg ccttgcagag gaccatgccg ccgcgcggcc cgcagagcgt
      901 cttcgtcgtg gacgaggtga cgaagtcggc gtgcggcacg gggctgggaa tgaccttcgc
      961 cgcgacgagg ccggcgacgt gcgccatgtc caccatcagc aggccgccgg cggagtgcgc
     1021 tatacgtgcg atccgctcgt agtcgatcag gcgcgggtag gcgctgccgc ccgcgatgat
     1081 cagcctgggc ttgtgctcct tcgccagcgc ctccagttcg tcgtagtcga tctgctcggt
     1141 ctgcgggttc agcccgtacg aaacgaagtg atagaatttg ctcatgaagt tcgccggaga
     1201 gccgtgcg
//
LOCUS       DAOSWW010000955         1208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_108940_length_1208_cov_2.952298, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000955 DAOSWW010000000
VERSION     DAOSWW010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108940_length_1208_cov_2.952298"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PK442_14540"
     CDS             <1..409
                     /locus_tag="PK442_14540"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOO88921.1"
                     /translation="PLLAESFGLHALSPAERAALDARAKKGDGAARTALLLALSGEEL
                     DELRAAFAQSVRAKEQRKKDRDYLRRYGPYEPLSVTVTRILNRKAGVEWSSFAHTGVD
                     VPVFAQGAGAARFAGEYDNTNVAEKILSVLGKR"
     gene            659..>1208
                     /locus_tag="PK442_14545"
     CDS             659..>1208
                     /locus_tag="PK442_14545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOO88922.1"
                     /translation="MKRSIVSDETRNKEAKKATWIGFVVNAFLTLFKILAGFLGGSAA
                     MIADGVHSLSDFFTDIVVLVGFKFTEKPADDEHNYGHGKYETLATVVISLALLLVGYK
                     IMTSGIVDIYEVVFKGKVLEQPEMIAFVAAVFSIASKELLFRYTRRVGEKIQSSAVIA
                     NGWHHRSDAFSSIGTMVGIGGAI"
BASE COUNT          264 a          311 c          380 g          253 t
ORIGIN      
        1 gccgctgctc gcggagtctt tcggcctcca cgccctctcc cccgccgagc gtgccgcgct
       61 ggacgccagg gcgaagaagg gcgacggggc cgcgcggacg gcgcttctgc tcgcgctctc
      121 gggcgaagag ctggacgagc tgcgggcggc tttcgcgcag agcgtgcggg ccaaggagca
      181 gcggaagaag gacagggact atctgcgccg ctacggcccc tacgaaccgc tttccgtgac
      241 ggtcacgcgt attctgaacc ggaaggcggg ggtagagtgg tcctccttcg ctcacaccgg
      301 agtcgacgtc cccgtcttcg cgcagggggc gggcgcggca cgcttcgccg gggagtacga
      361 caatacaaac gtcgccgaaa agatcctgtc cgttctcggc aaacgctagg cgaagcggca
      421 atccgaaact ccgagggccg gacggcggct ttttccgggg cttttgtgaa ccgaagcgca
      481 cacaagggct cgcaacacgt tgacaagtct attcactctt tatatactaa aacggcatca
      541 ggtgttgcat accgtgcagc agccgggccg aaaggcagca ggtacgacct gtgggactgt
      601 gatcgtcatt catggttcca cagtttttta ttcgtagaga acgaaggagc gtatcgctat
      661 gaagcggagt atcgtctcgg acgaaacgag aaacaaagag gcaaaaaaag cgacctggat
      721 cggcttcgtt gtgaatgcat ttttgactct ctttaagata ctcgctggat ttctcggcgg
      781 aagcgccgcg atgattgcgg acggggtgca ctctctgtcg gattttttta cggatatcgt
      841 ggtgctggtc ggttttaaat ttacggagaa acccgccgat gatgaacaca actacggaca
      901 tggaaaatat gaaacgcttg cgacggtcgt catcagtctg gcgctgctgt tggtcgggta
      961 taaaataatg acgtccggca ttgtggatat ttatgaagtt gtgttcaaag ggaaagtact
     1021 tgagcagccg gaaatgatcg cgttcgtcgc ggcggtgttc tcgatagcaa gcaaagagct
     1081 gttgttccgg tatacccggc gtgttggaga gaaaatccaa tcgtccgcgg tgatcgccaa
     1141 cggatggcac caccggtcgg atgcgttttc ctccatagga acgatggtcg gaatcggagg
     1201 agcgatat
//
LOCUS       DAOSWW010000956         1207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_109072_length_1207_cov_4.023438, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000956 DAOSWW010000000
VERSION     DAOSWW010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109072_length_1207_cov_4.023438"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PK442_14550"
     CDS             <1..321
                     /locus_tag="PK442_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88923.1"
                     /translation="RNGVWRCTPEDMNVEELEIEGFEWSPFSYFTDLFRGSGYAVFGV
                     KDDLLHRLLPKDGGYVMERASLSNVKVLAMLPLADGGILMGTSKAFYRSDDGGSTWKE
                     IGLP"
     gene            356..>1207
                     /locus_tag="PK442_14555"
     CDS             356..>1207
                     /locus_tag="PK442_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88924.1"
                     /translation="MRELYRRFGALLLLFLAVTFFLAVRDDVIRLYAVDMAEEYRSAY
                     LPVEGWGASTAMRGVVRSSKRISSVAAFIDAHTEGRLEVVEGKGWEEIFAGVLAPPET
                     SPLPDRRASDTWRASYYFLPKEDPFPEVFREMKSEDQAFRFLKLSAPSGPRFIGMTVQ
                     GPEDAMSAGAPSWILYPFRRFAAVPLLLALFVYLLFPPRKKAPAGALVYPKWSSWILP
                     DLMGLLFSCIFFAFPFFLAPAIFGSGNVLNIADGGIILTLVFWLMAAIFASILYWSAK
                     YASFSLEL"
BASE COUNT          185 a          376 c          390 g          256 t
ORIGIN      
        1 cgaaacggcg tctggcgctg caccccggag gatatgaacg tcgaggagtt ggagatagag
       61 ggattcgagt ggtcgccgtt cagctatttc accgacctct tccgcggaag cggctacgcg
      121 gtgttcgggg taaaggacga cttgctgcac cggctgcttc cgaaggacgg cgggtacgtc
      181 atggagagag cgtctctctc gaacgtcaag gtgctcgcca tgcttcccct tgcggacgga
      241 ggcattctga tggggacgtc gaaggcattc tatcgctcgg acgacggcgg aagcacgtgg
      301 aaggagatcg ggcttccatg agcggcccgg actctttgaa gaggaggcga ctggtatgag
      361 agagctgtac cgcagattcg gcgcgcttct gctgcttttt ttggccgtga cgttcttcct
      421 cgccgtccgc gacgacgtga tccgtctcta cgccgtcgac atggccgagg agtaccgctc
      481 cgcgtatctt cccgtggagg ggtggggggc gtccaccgcg atgcggggcg tcgtccgttc
      541 gtcgaagcgg atttcctccg tcgccgcgtt catcgacgcg catacggagg ggcggcttga
      601 ggtcgtcgaa ggaaagggct gggaagagat cttcgcgggc gtcctcgcgc ctcccgagac
      661 gtcgcccctg ccggacagga gggcgagcga cacctggcgg gcctcctact acttcctccc
      721 gaaggaggac cccttcccgg aggtgttccg cgaaatgaag agcgaggatc aagccttccg
      781 tttcctgaag ctctccgcac cgtcgggtcc gcgcttcatc ggaatgaccg tccaggggcc
      841 cgaggacgcg atgagcgcgg gcgcgccgtc gtggattctg taccctttcc ggcgctttgc
      901 cgccgtgccc ctgctcctcg cgctcttcgt ctacctcctc ttcccgccgc gaaagaaggc
      961 gcccgcgggc gcgctggtgt acccgaagtg gtcgtcctgg atcctccccg acctgatggg
     1021 attgctcttc tcctgcatct tcttcgcgtt tccgttcttc ctggcgccgg cgatcttcgg
     1081 cagcggcaac gtgctgaaca tcgccgacgg cgggatcatc ctcacgctcg tcttctggct
     1141 gatggcggcg atattcgcat ccatacttta ctggtcggcg aagtacgcct cattctctct
     1201 cgaactg
//
LOCUS       DAOSWW010000957         1206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_109340_length_1206_cov_1.461338, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000957 DAOSWW010000000
VERSION     DAOSWW010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109340_length_1206_cov_1.461338"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PK442_14560"
     CDS             <1..346
                     /locus_tag="PK442_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOO88925.1"
                     /translation="IVPDPHSIKINNPDAPVVTVFPKDGTLAWPAPAAIVKGTKNPEG
                     AKKFIDWALSPEGQKVLMLATPRVPVTDVEPIEGVPDPKALDLVPYDHVRWGAEREAV
                     LEEFSARYPHLN"
     gene            357..>1206
                     /locus_tag="PK442_14565"
     CDS             357..>1206
                     /locus_tag="PK442_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO88926.1"
                     /translation="MSVPHGFGRRASLLPFLFLLFLLLFVVHPSWTVLSRSVYVKGVP
                     GLGNLFALLERPHLFRIVWQSVLVAGGVALCATALGACIAVTVFRTRLPLRGLFRAAA
                     VLPLIIPGFVSSLSFLFLFGRNGLITYKLLKLDLAIYTWKSVFLIQVMDFATIAFLLV
                     SASLLTMDGRPEDAARTLGASETEVFRTVTLPLLRPGLLAAGILIFMRSMADFGTPLF
                     LGGRFATLASASYSQLIGSYNLEMASAMNTLLLLICLVAFLLFRRAQAAGERTRQTAE
                     RPPEPFA"
BASE COUNT          165 a          426 c          379 g          236 t
ORIGIN      
        1 gatcgtcccc gacccgcaca gcatcaagat aaacaacccg gacgcgccgg tggtcacggt
       61 cttcccgaag gacggcacgc tcgcctggcc ggcgcccgcg gccatcgtga aggggacgaa
      121 gaaccccgag ggagcgaaga agttcatcga ctgggcgctc tcccccgagg ggcagaaggt
      181 cctcatgctg gcgactccgc gcgttccggt gaccgacgtc gaacccatcg agggcgtgcc
      241 cgacccgaag gcgctcgacc tcgtccccta cgaccacgtg cgatggggcg cggagcggga
      301 ggccgtcctc gaggagtttt cggcgcgcta cccccatctg aactaggggg cgtttcgtga
      361 gcgtaccgca cggcttcgga aggagggctt ccctccttcc gtttcttttt ctgctctttc
      421 tgctgctctt cgtcgtccat ccgtcgtgga cggtgctgag ccgaagcgtc tacgtcaagg
      481 gcgttccggg cctcggcaac cttttcgcgc tgctggagcg gccgcacctc ttccggatcg
      541 tttggcaaag cgtcctcgtc gcggggggcg tcgcgctctg cgccaccgcg ctgggcgcgt
      601 gcatcgcggt gacggtcttc aggacgaggc tgccgctgcg cgggctcttc agggccgcgg
      661 cggtgctccc gctgatcatc ccgggattcg tctcctcgct ctccttcctc tttctcttcg
      721 gacggaacgg gctgatcacc tacaagctgc tgaagctcga cctcgccatc tacacgtgga
      781 agagcgtctt cctgatacag gtgatggact tcgccacgat cgccttcctg ctggtctcgg
      841 cgtcgctctt gaccatggac ggacgtcctg aggacgcggc gcgcacgctc ggcgcctccg
      901 aaacggaggt ctttcgcacc gtcacgctcc cgctgctccg gccgggcctc ctcgccgcgg
      961 gcatcctcat ctttatgcgc tccatggcgg acttcggcac gccgctcttt ctgggaggac
     1021 gcttcgccac cctggcgtcg gcctcctact cgcagctcat cggctcgtac aacctggaga
     1081 tggcctcggc gatgaacacg ctgctgctcc tgatctgcct ggtcgcgttc ctcctcttcc
     1141 gaagggcgca ggcggcgggg gagcggacgc ggcagaccgc cgaacgtcct cccgagccgt
     1201 tcgcct
//
LOCUS       DAOSWW010000958         1204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_109546_length_1204_cov_3.434291, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000958 DAOSWW010000000
VERSION     DAOSWW010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109546_length_1204_cov_3.434291"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PK442_14570"
     CDS             complement(<1..621)
                     /locus_tag="PK442_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOO88927.1"
                     /translation="MYCFQCQETAKNQGCTVKGVCGKPEGTANLQDLLIYVCKGIAVY
                     GEKLKQQGTVDREAGRFIIRALFVTITNAAWDDDSIIEWIRKGLKVKDAVAQKAGAAG
                     NLPDCATWKAESKEEILAKAMSAEVRVTAAQNEDVRSLRELVIIGGKGVAAYAEHAEV
                     LGFEKDEIYSTLMEGLASTTKDLSTDEMVGMVMKIGEIAVTTMALLD"
     gene            complement(699..>1204)
                     /locus_tag="PK442_14575"
     CDS             complement(699..>1204)
                     /locus_tag="PK442_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOO88928.1"
                     /translation="LPCGCPGSAARDLRKTPSATPQARPEAASAPLQSELVNWPVQIR
                     LVPETAPWLHKAELVIGADCVPFAFPDFHRTFLAGGDKVCLIGCPKLDDAQSYVEKLT
                     RIISYNETPAITVVRMEVPCCGGMTRILEAACEASERNISLKIVTIGIRGDILDKETI
                     RFSRRQP"
BASE COUNT          207 a          376 c          362 g          259 t
ORIGIN      
        1 gtcgagaagc gccatcgtgg tgacggcgat ctcgccgatc ttcataacca tgccgaccat
       61 ctcgtctgtg gagaggtcct ttgtggtgga agcaagccct tccatgagcg tcgaatagat
      121 ctcgtccttc tcgaagccga ggacctcggc gtgctcggcg taggccgcga cgcccttgcc
      181 gccgatgatg acgagctccc gcagcgagcg gacgtcctcg ttctgcgcgg cggtgacgcg
      241 aacctcggcg gacatggctt tcgcgaggat ctcttccttg ctctcggcct tccacgttgc
      301 gcaatcggga aggtttccgg cggcgccggc cttctgcgcg accgcgtcct tgaccttcag
      361 ccccttgcgg atccactcga tgatggagtc gtcgtcccac gccgcattgg tgatggtgac
      421 gaaaagcgcg cgaatgatga aacggccggc ctctctgtcc accgtcccct gctgcttcag
      481 cttttcgccg tatacggcga tgcccttaca gacgtagatg agaaggtcct gaagattggc
      541 ggtgccctcc ggctttccgc agactccctt tactgtgcac ccctgattct tcgctgtctc
      601 ctgacactga aaacaataca tgccggcgct tcctcctttg tgaatttgat tttttcgaaa
      661 catgccgagt acccgttgat acgtctccga tagtgcgctc atggctgtct cctggagaac
      721 ctgatggtct ccttgtccag tatatcacca cgaataccta tcgtcacaat tttcagcgat
      781 atgttccgct ccgaggcctc gcatgccgcc tccagaatgc gcgtcatccc gccgcagcag
      841 gggacttcca tacgcaccac ggtgatcgcg ggcgtttcgt tgtatgagat aatccgcgtg
      901 agcttttcga cgtagctctg cgcgtcgtcg agcttggggc agccgatgag gcagacctta
      961 tcgcccccgg cgaggaaggt tctgtggaag tccgggaagg cgaacggaac gcagtcggcg
     1021 ccgatcacaa gttccgcctt gtggagccag ggcgcggtct ccggcacgag gcgtatctgc
     1081 accggccagt tgacaagctc cgactgaaga ggcgcggacg ccgcctcggg gcgcgcctgc
     1141 ggggtggcgg acggagtttt ccgcagatcg cgggccgccg aaccggggca gccgcaggga
     1201 agag
//
LOCUS       DAOSWW010000959         1200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_110225_length_1200_cov_2.856769, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000959 DAOSWW010000000
VERSION     DAOSWW010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110225_length_1200_cov_2.856769"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PK442_14580"
     CDS             complement(<1..1090)
                     /locus_tag="PK442_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO88929.1"
                     /translation="MTKSPRLGDILVLSGAISDEQLQSALKEQKSSGLKLGELLLHKK
                     IITEGKLMEALATQLRLQIYSLSRYRPMPEALRMVPQGVAKRLQVVPLAVEDGNTLLV
                     AMADPLDVLAQDEIRMISGMELRIGIVSPTELERNLERLYSIQESLEGANLEFMDSSP
                     RDVDLTQAMGATTPDDAPVIQLVNNIIEQAVRENCSDIHIEPFEKNTRVRYRIDGQLF
                     NALDFQKNLHPAVSSRLKIMSGMDISEKRKPQDGRILIKILGRRVDLRISSLPTIYGE
                     KIVIRILDQENAMVGLKKLGLEDDDKQKLDYICEAPYGIVLVTGPTGSGKSTTLYSVL
                     EQINKPEVNIVTVEDPVEYTLFGIGQVQV"
BASE COUNT          241 a          295 c          363 g          301 t
ORIGIN      
        1 tgacctgcac ctgtccgata ccgaagagcg tgtactccac cgggtcctcc actgtgacga
       61 tgttcacctc cggcttgttg atctgttcga gtacggaata gagggtagtg gactttccgc
      121 ttccggtggg gcctgtgacg aggacgatgc cgtagggagc ttcgcagatg tagtcgagct
      181 tctgtttgtc gtcgtcttcc agaccgagtt ttttgaggcc gaccatggcg ttctcctggt
      241 caaggattcg gatgacgatc ttttccccgt agatggtcgg gagcgaggag atacgaaggt
      301 cgactctgcg tccgagtatt tttatgagta ttcgtccgtc ctgaggtttt cgcttctcgg
      361 agatgtccat cccggacatg atcttgagcc gcgaggagac ggcggggtgg aggttttttt
      421 gaaagtcgag ggcattgaag agctgcccgt cgattcgata gcgcacacgg gtgttctttt
      481 caaagggttc gatgtggatg tccgaacagt tttcgcggac tgcctgttcg atgatattgt
      541 tgacgagctg gatgaccggg gcatcgtcgg gggttgttgc gcccatggcc tgcgtgaggt
      601 cgacgtcgcg cggggaggaa tccatgaatt cgaggttggc gccttcaagc gattcctgta
      661 tggagtacag acgctcaagg tttcgctcca gctccgtggg cgaaacgatg ccgatgcgca
      721 gctccatccc ggagatcatc cggatttcgt cctgcgcaag aacatcaagg ggatccgcca
      781 tggcgaccag aagcgtattc ccgtcttcga ccgcgagagg aacaacttga agacgttttg
      841 ccacgccttg cggtaccatt cgcaacgctt caggcatcgg ccggtagcgg gaaagcgagt
      901 atatttggag cctgagctgc gtcgccaacg cttccatcaa cttgccttca gtgatgattt
      961 ttttatgaag cagcagctcc ccaagcttca aaccggaact tttctgctcc ttgagcgcgg
     1021 attgcagctg ctcgtcggag attgctccgg acagtacaag aatatctccc agtcgaggcg
     1081 atttggtcat gctctctccc ctttatctcg atgacatgtt tgatgctgcg ggattgtatg
     1141 ggacgataat agcgtaaaag ggggaaatgc acaacgagag gggaacaggc atagaggatt
//
LOCUS       DAOSWW010000960         1200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_110229_length_1200_cov_2.820087, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000960 DAOSWW010000000
VERSION     DAOSWW010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110229_length_1200_cov_2.820087"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1200
                     /locus_tag="PK442_14585"
     CDS             <1..>1200
                     /locus_tag="PK442_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOO88930.1"
                     /translation="RGKEHASKHPGLEQLAEGMNTAEEQVTLYQQLLVESQGHMANIA
                     TQRRHAAEAAASMGAVAYDLLFDEKDELEKETEEGADADTLLDRQESILLLDDLVEAV
                     HKAESYTLRSIAERRIELLDRVEDELKRVESLLKKVQATAVKQRQKDQLKKGFEDVES
                     YKGAIASFREAWKDLDEMGKTRTAAAEGVLAAAAKVMNFSVERTLQIAGVVTGQARDV
                     SGMLIASVLFALFAGIVVAFLITRMITRPLHRAVALAERAGGGDLTIERKDFGHEGKD
                     EIGILADALAMMVKNQADSVRNVVAAAHEISQGAGALAALSQETNASVEEVRSSLEQV
                     ASISESNSAALEESNAGVEEVAAGAQASAKASSEGVAAAEHTAAVAKEAVDRVADVIE
                     DVHSVGGK"
BASE COUNT          256 a          318 c          425 g          201 t
ORIGIN      
        1 cgtggtaagg agcacgcttc gaagcacccc gggctggaac agcttgccga agggatgaat
       61 acagccgagg agcaggtcac gctctatcag cagctccttg tggagtcgca ggggcatatg
      121 gcgaacatcg ccacgcagcg caggcatgcg gcggaagctg ccgcttccat gggggccgtc
      181 gcctacgatc tccttttcga cgagaaggac gaactggaga aggagaccga ggagggagcc
      241 gatgccgaca cgctgctgga ccgccaggag agtatcttgc tcctggacga ccttgtcgag
      301 gcggtgcaca aagcggagag ctatacgctc cgttctatcg ccgaacggcg gatcgagctg
      361 ttggataggg ttgaagacga attgaagcgg gtcgagtcgt tgctgaagaa ggtgcaggcg
      421 acagccgtca agcagaggca gaaagaccag ctgaaaaagg gctttgaaga cgtggagtcg
      481 tacaagggcg ccatcgcgtc cttcagggaa gcatggaagg atctggacga gatgggaaag
      541 accaggacgg ccgcagccga gggagtgctg gcagcggcgg ccaaggtgat gaacttcagc
      601 gtggagcgca cgctgcagat cgccggagtc gtgaccggac aggcgcgcga tgtctccggg
      661 atgctgattg cttccgtgct ttttgcgctc tttgcgggca ttgtcgtcgc gttccttatc
      721 acgcgcatga ttacccgtcc tctacacagg gcggtcgcgc ttgcggagag agcaggcggc
      781 ggggatctta ccatagagcg gaaggacttc ggtcatgaag ggaaggatga aatcggcata
      841 ctggccgacg cccttgccat gatggtgaaa aaccaggcgg attcggtccg caatgtggtc
      901 gccgccgccc atgaaatttc ccagggcgcc ggcgccctgg ccgctctgtc tcaggagacg
      961 aacgcctcag tggaggaggt gcgcagttcg ctggagcagg ttgcctcgat ctccgaaagc
     1021 aactctgccg cactggagga atcgaacgcc ggagttgaag aagttgccgc aggggcccag
     1081 gcctccgcga aggcctcctc ggagggagtg gccgcggcgg agcatacggc ggctgtggca
     1141 aaggaggcgg tggaccgggt tgcagacgtc atcgaagatg tccactccgt cggcggcaag
//
LOCUS       DAOSWW010000961         1199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_110344_length_1199_cov_3.555944, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000961 DAOSWW010000000
VERSION     DAOSWW010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110344_length_1199_cov_3.555944"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..451
                     /locus_tag="PK442_14590"
     CDS             119..451
                     /locus_tag="PK442_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO88931.1"
                     /translation="MQLQKGNLAELPANQPDEEIFEELLRTSSFRVERIVSTGQASPP
                     GFWYDQEEDEWVALLQGEAVLEFDDGSAETLRSGDWVFLPAHIRHRVASTSLEPPCIW
                     LAVFARPG"
     gene            666..>1199
                     /locus_tag="PK442_14595"
     CDS             666..>1199
                     /locus_tag="PK442_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO88932.1"
                     /translation="MIRRTLLFVHLFFFIYGASFAAALPIDEFIEVCGAGSLEEVHEA
                     VASGADVNATDADGWTALMAAADNGNTEVVAFLVDRGADIHRKDRCGTTALMAAAGGS
                     DTRSVQLLVNAGGNVNERNSGGWTPLFFAARFSEEPDVIALLIASGAEVDVAEADGET
                     PLMIAAWYGENPRIVAAL"
BASE COUNT          251 a          285 c          362 g          301 t
ORIGIN      
        1 cgccggagca ttctcgcaga tcgtcttcgc catcgccgac tggtcttcgg aaaggcgctt
       61 tcttggtcct ttccgggatg tcttcgctgc ggagaatggc tgatcgaagg aggaaaaaat
      121 gcaacttcaa aaaggcaacc ttgcagagct tccggcaaat cagccggacg aggagatctt
      181 cgaagagctt cttcgcacat cgtctttccg cgtggaacgg atcgtctcca caggccaggc
      241 gtcgccgccg ggcttttggt acgatcagga ggaggacgag tgggtggcgc tgctccaggg
      301 cgaggcagtg ttggagttcg atgacggttc ggccgagacg ctccgtagcg gcgactgggt
      361 ttttcttccg gcgcacatcc gccacagagt agcgtcgacg agcttggaac cgccctgcat
      421 ctggctggct gtttttgccc gcccgggata actatcgaag tgaacggtga aaatcgcatc
      481 tccgaagagt ttgcgatctg tacattctta aaaaataaaa attgtgtttt tgtgttctag
      541 atctctccat atcaagcgga atcctctatg ccacgcgaaa attttgtgat ttttaatgta
      601 attcgtgtat gattgtaacg agcctcctca tagatgttgc ctcttcatca ggaagggtga
      661 gttcgatgat tcgaagaacg cttttgttcg tgcacctttt ctttttcatc tacggcgctt
      721 cttttgcagc ggctctacct atcgatgaat ttattgaggt atgcggcgcc ggttctcttg
      781 aggaagtgca tgaggccgtc gcttcagggg ccgatgtaaa tgcaacggat gcggatggat
      841 ggaccgcttt gatggcggcg gcggacaacg gaaataccga ggtcgttgca tttttggttg
      901 accgcggggc ggatattcat agaaaagaca gatgcggaac aacagcgctt atggcggctg
      961 ccggcggttc ggatacgcgt tcggtacaac tccttgtaaa tgcgggaggg aacgtgaatg
     1021 aacggaattc gggagggtgg acgccgcttt tcttcgcagc ccgtttcagc gaggaacccg
     1081 acgtgatcgc gcttctgatt gcatcgggcg cggaagtcga cgtagctgaa gcagacggtg
     1141 agacgcctct tatgatcgcg gcatggtacg gcgagaatcc gcgtattgtg gcggctttg
//
LOCUS       DAOSWW010000962         1198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_110481_length_1198_cov_3.928259, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000962 DAOSWW010000000
VERSION     DAOSWW010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110481_length_1198_cov_3.928259"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PK442_14600"
     CDS             <1..373
                     /locus_tag="PK442_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88933.1"
                     /translation="ARISGAEGGRLVLADSMRPSGFVPAELDRAVDVDALSGGEKEQL
                     HFAVRMALADCSGREERRLLVLDDTLMATDGIRFPRVLDIIEEAAEELQILILTCQPE
                     RFEPLRKALRIDLGALLGRTA"
     gene            417..>1198
                     /locus_tag="PK442_14605"
     CDS             417..>1198
                     /locus_tag="PK442_14605"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO88934.1"
                     /translation="MSEQNMLERIKAFHTHCTTASPGEIAEALRNGTSPDARDEDRMT
                     ALMWAARRNPDPEAVRTLLAAGAELEARDESGLTPLMWAARNNARPEVVSALLAAGAD
                     MDARNLAGQTALIVAAEHTTNPEVLRTLLAAGAEIEARDFRGSTALMEAAQSNDNSGA
                     TRALLDAGANVDARDDSGGTALMRCVSICSVNSSDTGLVGMLISAGADVNARNDRRRT
                     VLMEALEDGLNAGVVEMLLKSGADIHARDEDGRTPLLCATRVL"
BASE COUNT          223 a          383 c          417 g          175 t
ORIGIN      
        1 tgcccgtatc tccggcgccg agggagggcg ccttgtactt gcggattcga tgagaccctc
       61 ggggttcgtc cctgcggagc ttgacagggc cgtcgacgtc gacgcccttt ccggagggga
      121 gaaagagcag ttgcacttcg ccgtccggat ggcgctggcc gactgctcgg ggagggagga
      181 acgacgtctc ctggtattgg acgacacgct catggccacg gacggcatcc gtttcccgcg
      241 cgtcctcgac atcatcgaag aagcggcgga ggagctgcag atactgatcc tcacctgcca
      301 gcccgagcgt ttcgagcccc tgaggaaggc tctcaggatc gacctcgggg cgctgctcgg
      361 gcgaacggcc tgaggaaccg gaacgtttcc atgaactctt gaggggaggc ggtaccatga
      421 gcgaacaaaa catgctggag cgcataaagg cctttcacac gcactgcaca accgcctctc
      481 ccggggagat cgcggaggcg ctacgaaacg gaacctcacc ggacgcccgc gacgaggaca
      541 gaatgaccgc gctcatgtgg gcggcgcgaa ggaacccgga tccggaggcg gtgcgtacgc
      601 tgctcgccgc cggagccgaa ctggaggctc gcgacgaaag cgggctgacg ccgctgatgt
      661 gggccgcccg aaacaacgcg cgccccgagg tcgtctccgc gctgctcgcc gccggagccg
      721 acatggacgc ccggaacctc gccgggcaga ccgcgctgat cgtcgcggcg gaacacacga
      781 cgaaccccga agtccttcgg acgctccttg cggcgggcgc ggagatcgaa gcccgagatt
      841 tccgtggctc gaccgccctg atggaggccg cgcaatccaa cgacaactcc ggggcgacgc
      901 gcgcgcttct ggacgccggg gcgaatgtcg acgccaggga cgacagcggg gggacggcgc
      961 tgatgcgctg cgtttcgata tgctccgtga acagctccga tacggggctt gtaggaatgc
     1021 tcatctcagc gggcgccgac gtcaacgcgc gaaacgaccg gaggcgtacc gtccttatgg
     1081 aggcgttgga ggacggactg aacgccgggg tcgtcgaaat gctcctgaag tccggcgccg
     1141 acatccacgc gagggacgaa gacgggcgga cgccgcttct gtgcgcgacc cgcgttct
//
LOCUS       DAOSWW010000963         1198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_110486_length_1198_cov_3.445319, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000963 DAOSWW010000000
VERSION     DAOSWW010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110486_length_1198_cov_3.445319"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PK442_14610"
     CDS             complement(<1..686)
                     /locus_tag="PK442_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOO88935.1"
                     /translation="MKRFAKVMFAVALVLALGTAAFAAPKIGIMTGTVSQGEEEYRAG
                     EEMVAKYGADRVIHVTYPDKFMDEQETTISQVMAMASDPDLKAIVICQGVPGTAAAID
                     KVKEVRDDIAFIVGTVHEEPYMMAGKADILYEIDQPARGSSIIEKAKGMGAKTFIHYS
                     FPRHMSYPLLATRRDIMEKTCKELGIEFVFVNAPDPTGEGGVAGAQQFILEDAPRQIE
                     KYGPDTAFFST"
BASE COUNT          273 a          283 c          319 g          323 t
ORIGIN      
        1 gtgctgaaga acgccgtatc ggggccgtac ttctctatct ggcgaggagc atcttcaagg
       61 atgaactgct gtgcgcccgc aacgccgcct tcaccggtgg ggtccggagc gttgacgaaa
      121 acgaactcga tgccgagctc tttgcacgtc ttttccatga tgtctctgcg ggttgcaagc
      181 agaggatagc tcatgtggcg ggggaaggag tagtggataa aggtcttcgc gcccataccc
      241 ttcgccttct cgataatgga gctgccgcgg gcgggctggt cgatttcgta gagaatgtcg
      301 gctttgccgg ccatcatgta gggttcctca tgaacggtgc cgactatgaa ggcgatatcg
      361 tcgcgcactt ctttgacctt gtcgatggcg gccgcagtgc cgggaacgcc ctggcagatg
      421 acgatggctt tcaggtcggg atccgacgcc attgccatga cctgggaaat tgtcgtttcc
      481 tgctcgtcca taaacttgtc aggataggtt acatgaataa ccctgtctgc gccgtacttg
      541 gcaaccattt cttctccggc gcgatactct tcctcaccct gggaaaccgt tccggtcatg
      601 atgccgatct tcggggcggc gaaagccgcc gttccaagag caagaaccag cgcaactgcg
      661 aacattactt tggcgaagcg tttcatacaa atatctcctc cttcatgatt atggtgcaaa
      721 actttgttca gctatgagtc taaaagaaaa atatgttaaa atgggcattc ttatactcat
      781 agtttaataa tatgctatac catcttccga tatttgtaaa gagtttcggt ggtttgcgca
      841 tcgccgtgtt tcatctggcg gggaattgag caaaaatgaa agaccatgct gcatcatgaa
      901 aaagtcgtaa gtgcgctatt tattcattga aaaggtatgg atgaatcctg taagtctatt
      961 atataatctc ctcatagtct atgttgcgct ggggatgcag gttctgttct ctacgcgggt
     1021 atatgctgct caataatgga gaaaagaggt aatgatattg ttgaagaaaa catgttccat
     1081 cgtttgcgct accctcttcc ttgcgtgggg aatcgttgct gcaaacgcct cggagatgtc
     1141 tcgggagggg gttattcttg cagtacgcga gaataacccg acgcttcagg cgtctctt
//
LOCUS       DAOSWW010000964         1196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_110832_length_1196_cov_2.560911, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000964 DAOSWW010000000
VERSION     DAOSWW010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110832_length_1196_cov_2.560911"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PK442_14615"
     CDS             complement(<1..657)
                     /locus_tag="PK442_14615"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--AMP phosphotransferase"
                     /protein_id="HOO88936.1"
                     /translation="MLEAVDMSVKMKKKEYKAAFDELAPKLGELQRIAKEKGVPVILL
                     VEGWEGSNKGSLVNQLLQALDPRGYLFIAPPGRGEERSPFLIPYWEITPPAGKMTILS
                     GSWYREVLRRRLEKGEGAKDYSGVFRDICAFERHLADSGTVVLKFFCHLEKKEQKKRL
                     EARAADPDLSWSVGEADWLENREYDAFLFCAEEMLSFTGTEYAPWTVVAAGDFEFAAW
                     K"
     gene            complement(705..1070)
                     /locus_tag="PK442_14620"
     CDS             complement(705..1070)
                     /locus_tag="PK442_14620"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOO88937.1"
                     /translation="MISSVDEEGVPNTKAMLPPRKREGIKRLYFTTNTSSMRVQQYGV
                     NPKACVYFFDKRFFRGVMLKGTMEVLHDPETKETIWRDGDTMYYPGGVTDPDYCVLRF
                     TAESFRFYSDFKSESCAIE"
BASE COUNT          236 a          400 c          316 g          244 t
ORIGIN      
        1 cttccacgcg gcgaactcga aatcccccgc cgcgaccacc gtccacggcg cgtactcggt
       61 ccccgtgaag gagagcatct cctccgcgca gaagaggaaa gcgtcgtact ccctgttctc
      121 cagccagtcg gcctcgccca ccgaccagga gagatcgggg tcggcggcgc gggcttcaag
      181 gcgcttcttc tgctccttct tctccaggtg gcagaagaac ttgagcacca ccgtgccgct
      241 gtcggccaga tgacgctcga aagcgcagat gtcgcggaag acgccggagt agtccttcgc
      301 cccctcgccc ttctccagcc tgcggcggag cacctcgcgg taccagctcc ccgagaggat
      361 cgtcatcttt cccgcgggcg gcgtgatctc ccagtacgga atcaggaagg gagagcgctc
      421 ctcgccgcgc cccggaggcg cgatgaagag gtaaccgcgg gggtcgagcg cctggaggag
      481 ctggttgacg aggctcccct tgttcgagcc ctcccaccct tcaacgagca ggatgacggg
      541 aacccccttc tccttggcga tccgctgcag ctccccgagc ttcggcgcca gttcgtcgaa
      601 ggccgctttg tactccttct tcttcatctt caccgacata tccaccgctt caagcattgt
      661 tccgcctccc ttccgtagat ttactcaacg ccgtcgtctg aaattcactc gatcgcacaa
      721 ctctcggact tgaaatcgct gtaaaaccgg aaactctcgg cggtgaatcg caagacgcag
      781 taatcggggt ccgtcacccc tcccggatag tacatcgtat cgccgtcccg ccagatagtc
      841 tctttcgtct ccggatcgtg cagcacctcc atcgtgccct tcagcatgac gcctctgaaa
      901 aagcgcttgt cgaagaagta gacgcaggcc ttcggattca ccccgtactg ttgcacacgc
      961 atggaggacg tattcgtcgt gaaatagaga cgcttgatcc cctctctctt tcggggaggg
     1021 agcatcgcct tggtattggg aaccccctct tcgtcgacag agctgatcac cgacaccggg
     1081 ccttgtcgat caagttcccc accgtctttt caggatcccg cattgcaaag gcctccttta
     1141 atagcacggt actcttgcga aggataaaca actgaaaaag agcgccgtaa aagaat
//
LOCUS       DAOSWW010000965         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_111028_length_1195_cov_2.121930, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000965 DAOSWW010000000
VERSION     DAOSWW010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111028_length_1195_cov_2.121930"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PK442_14625"
     CDS             <1..506
                     /locus_tag="PK442_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88938.1"
                     /translation="YIPSMFTRVSVVTWSANVFGFRAPSGRMDDAAPLFRVIHQSRAE
                     NPQWHTNYIRLAATTARDQIRMQQQSFAAMQRVRQTLNETSDIIARGYEERSRAMDRV
                     FDDYSRSIRGTELYSDPYAGRTVELPNGYREVWTDGTDYILSDNANFNPNIESLQNWR
                     RMETTKV"
     gene            653..>1195
                     /locus_tag="PK442_14630"
     CDS             653..>1195
                     /locus_tag="PK442_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88939.1"
                     /translation="MKQSVFHRCAKRTALCAGLVVLLCVFAPAGFSAGEAAPPKIPLK
                     EAALKDFSSVPSPMKLDVVPNFKETAFPLDKAANLKEVEEVVGKLTAEQKSALQKNRF
                     LLLQKPSLHTFSSMGRYDEMLCNFEGIGGSYDPAYREPWNTRFVGPDVFLHALHTFFS
                     KRLEAMEGGELLGAVQYMLEE"
BASE COUNT          248 a          347 c          381 g          219 t
ORIGIN      
        1 cgtatattcc ttctatgttt acccgcgtat cggtggtcac ctggtcggcg aacgtcttcg
       61 gattccgcgc gccctccggg aggatggacg acgcggcccc tctttttcgc gtcattcatc
      121 agtcgcgggc cgagaaccct cagtggcata cgaactacat ccgccttgcg gcgacgactg
      181 cgcgcgacca gatccggatg cagcagcaat cgttcgcggc catgcagcgg gtacggcaga
      241 cgctgaacga gacaagcgac atcatcgccc gcggctacga agagagaagc cgcgcgatgg
      301 accgcgtgtt cgacgactac agccgctcga tacggggcac ggagctgtac tccgacccct
      361 acgcaggacg gacggtcgag ctgccgaacg gctataggga ggtatggacc gacggaaccg
      421 actatatcct gagcgataac gccaacttca accccaatat cgagtcgctg cagaactggc
      481 ggcggatgga gacgacgaaa gtgtaagagc tggttgctta cgagaaaggt tgcggtgtgg
      541 cgcttcgtct acgggaggac cacttccgtg aagttccggc ggaaggcgct ccggaatgct
      601 ccgcacgctg cggaaaatcg tgttccaacg agccgaaaag gaggaatgtg gcatgaaaca
      661 gagcgtgttc caccgatgcg cgaagcggac ggcgctgtgc gcaggtctgg ttgtgcttct
      721 gtgcgttttc gcgccggcag gtttctcggc aggagaggcc gcgccaccga agattccgct
      781 gaaggaggcg gcgctgaagg atttttccag cgtcccgtcc ccgatgaagc tcgacgttgt
      841 tcctaacttc aaagagacgg cgttcccgct cgacaaggcg gcgaacctca aagaggtgga
      901 ggaagtcgtc ggcaagctca cggccgagca gaagagcgcg ctgcagaaga accgcttttt
      961 gctgctgcag aaacccagtc tgcatacttt ttcgtcgatg gggcggtacg acgaaatgct
     1021 gtgtaacttc gaaggcatcg ggggaagcta cgatcccgcc taccgcgagc cgtggaacac
     1081 ccgcttcgtc ggcccggacg tctttctcca cgcgctccac accttcttct ccaagcgcct
     1141 tgaggcgatg gagggaggcg agctcctggg cgcggtgcag tacatgctcg aggag
//
LOCUS       DAOSWW010000966         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_111066_length_1195_cov_1.583333, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000966 DAOSWW010000000
VERSION     DAOSWW010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111066_length_1195_cov_1.583333"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..>1195)
                     /locus_tag="PK442_14635"
     CDS             complement(392..>1195)
                     /locus_tag="PK442_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88940.1"
                     /translation="GCVVRVGALSRSAGIHPNNIRVQLDRLAESGLIESVRHGLDGRW
                     IRISCRDDSNEFAAIIKPCSSSVVNKTTTLLLQKNTNENVGSNEFIGSNVFAASNEFV
                     ATGGIAPTGKFASILKNKKRSSILEGLFAAALSAGKAPEDLQRQIIERLVALAEQKSP
                     QIAMATICEFLPPAKSNPGAYLARVLDAGAEPSPARLADAEKIAEALRSFRMEIGVEI
                     LPADWCKASRVLGIVAKPEESSRAQKATLSRLDAFVAALLPNYNASSSG"
BASE COUNT          268 a          304 c          368 g          255 t
ORIGIN      
        1 aaatcgccct ccgccaatat acattaaccc tcaaggttaa tataaaaatc ttctacagat
       61 gattatacca cggatgagaa gcgatgaaag aagatattgg aagattccta tgatccgatg
      121 agatgaaaaa accccggact ctatgtccgg ggttcaacgc ctttcggcag tcagaggatg
      181 agatcgttcg agagcctgca tcgacaacgc acaagaaata gtgctcaacg cctttcggca
      241 atcggagaca aaaagcgccg ggagaaaagg tggtacggga aactcccggc gcgtgaaaca
      301 ggcgaaagta ttgtaccacg aaaaaaggcc cgcgccgttg gcatgagaca cgcgcggtgt
      361 ggcgcttcga cggcgtgttg cggagaggcg tctatccgga tgaggaggcg ttgtagtttg
      421 gaaggagcgc ggcgacgaag gcgtcgagcc gcgagagcgt cgccttctgc gctctggagg
      481 attcctccgg cttcgcgacg atcccgagga cgcgggaagc cttgcaccag tcggcgggga
      541 ggatttcgac gccgatctcc atgcggaagg aacgcagcgc ttcggcgatc ttttccgcgt
      601 cggccagccg cgccgggctt ggttcggctc cggcgtcgag gacgcgggcg agatacgctc
      661 ccggattgga cttcgcgggg ggaagaaatt cgcagatcgt cgccatcgct atttgagggc
      721 tcttctgctc cgcgagagcg acaaggcgct cgatgatctg gcgctggagg tcttcggggg
      781 ctttcccggc ggaaagtgcg gcggcgaaga gcccttcaag gatggatgaa cgttttttat
      841 ttttgaggat ggaggcgaat ttgcctgtag gggcgattcc gcccgtagca acgaattcgt
      901 tgctagctgc gaaaacgttg ctaccaatga attcattgct accaacgttt tcgttggtat
      961 ttttctgtag tagtagtgta gttgttttat taactacact actactacaa ggctttataa
     1021 tagctgcgaa ttcgttgcta tcatccctgc acgagatgcg aatccagcgc ccgtcgagcc
     1081 cgtgacggac ggactcgatc agaccgctct ccgcaaggcg gtccagctgt acgcggatgt
     1141 tgttcgggtg tattccggcg gaccgggaga gcgctccgac ccggacgacg catcc
//
LOCUS       DAOSWW010000967         1194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_111161_length_1194_cov_2.471466, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000967 DAOSWW010000000
VERSION     DAOSWW010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111161_length_1194_cov_2.471466"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PK442_14640"
     CDS             complement(<1..997)
                     /locus_tag="PK442_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88941.1"
                     /translation="MIIEEEKAITETEKSVPTASTVDRDGQWKEVSCEFFDDLLAAAL
                     PELLEAMDTDKRPEFQEQELRAVATTTGGKERNIDLLAKVALKSGKNVHVLLHIEIQD
                     SDDDDFPERMFRYRSLLMARYLKSYEDKGRAVLVNDKAEDIVSLAILTARRPKREKEY
                     YEHSSFRNTLRFEYPALKVWECDPAEMANSENPFHWVLLSGRRVLESGRNDAKKVRYL
                     RELNDILKEKRWSRRKKERLFIFMEALLRPKEEEALKEYRKYLEEVRTKEGEKMAYVS
                     VPEEMGIEKGIEKGIVIGAEGTKRENALRMLRKGLDIDLIAECVDLPEEEVRRLAD"
BASE COUNT          196 a          380 c          332 g          286 t
ORIGIN      
        1 cgtcggcgag ccgacgtact tcctcctcgg ggaggtcgac acactcggcg atgaggtcga
       61 tgtccagccc cttgcgcagc atccgaaggg cgttctcgcg cttggttcct tccgctccga
      121 tgacgatccc cttttcgatt cccttttcga ttcccatctc ttcgggaacg ctcacatatg
      181 ccatcttttc tccctccttc gtcctgacct cttcgaggta cttccggtac tccttgaggg
      241 cttcctcttc cttggggcga agaagcgcct ccatgaagat gaagagccgc tccttcttgc
      301 gccgggacca ccgcttctcc ttcaggatgt cgttcagctc ccgcaggtag cgcaccttct
      361 tcgcgtcgtt ccgcccgctt tcgagaaccc ttcgcccgga cagcagcacc cagtggaagg
      421 ggttctcgga gttcgccatc tcggcggggt cgcactccca gaccttgagc gcggggtatt
      481 cgaatcgcag cgtgttgcgg aaggaggaat gttcgtagta ctccttctct cttttcgggc
      541 ggcgcgcggt gagtatcgcc agggagacga tgtcctcggc cttgtcgttg acgagcactg
      601 ccctgccctt gtcctcgtag ctcttgaggt agcgcgccat gagaagcgat ctgtaacgaa
      661 acatcctctc gggaaagtcg tcgtcgtcgc tgtcctgtat ctcgatgtgg agcagcacgt
      721 ggacgttctt ccccgacttc agcgcgacct tcgccagaag gtcgatgttc cgctccttcc
      781 cgccggtcgt cgtcgcgacg gcgcgaagct cctgctcctg aaactcgggt cttttgtcgg
      841 tgtccatcgc ctccagaagc tcgggaagcg ccgctgccag caggtcgtcg aagaactcgc
      901 acgagacttc cttccactgc ccgtcgcggt ccacggtcga agcggtcggc acgctctttt
      961 ccgtctccgt aatggccttc tcctcctcga tgatcaagct gcattttcat ttgaaagaat
     1021 tgtagcacat tgcgcggtgc ggggaaagag gcaagcgcgc gtcacgatat tactcgcttg
     1081 catttgacta agcgtatccg aaaggataca gcaagacgca tgactgttat catgcgtacg
     1141 ccggagtgcg gaatcaggga gcggcgctcg cgcagcagtt cgctcaccgt ctgc
//
LOCUS       DAOSWW010000968         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_111277_length_1193_cov_3.769772, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000968 DAOSWW010000000
VERSION     DAOSWW010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111277_length_1193_cov_3.769772"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PK442_14645"
     CDS             <1..1079
                     /locus_tag="PK442_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO88942.1"
                     /translation="DAALRFGPAEMFSLMLFALVATITLAEGSLLKGVIAMCIGFMLS
                     TIGIDALTGILRFTYGVEELQDGIDFLVVMIGVYAICEVFKNLKNLETKYNLDSKSIG
                     KVWVSWPQFKKCIMPMLRSSPLGFFVGVLPGMGASIATFMSYAMEKNLSKTPEKFGKG
                     AIEGLAAPEAANNSCAAGAMVPLLTLGIPGSGTTAVMLGALMMLGVKPGPILFSQYPE
                     IAWGVIASILIGNIILVIINLPLAIPLVQLLKIPQRIMMPLILGMAFMGTYFLNYSSF
                     DFILVMGFALGGYIFSKLEIPLPPMILALILGGTTEQSFRNSMSLSDGSLGIFFSKPI
                     SVTLIALAGLSLVYSLLKRRKHAQ"
     gene            1076..>1193
                     /locus_tag="PK442_14650"
     CDS             1076..>1193
                     /locus_tag="PK442_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOO88943.1"
                     /translation="MTTFLDRPRRSLLYIPGNNPGMIQNCAIYGSDGVLLDLE"
BASE COUNT          222 a          383 c          328 g          260 t
ORIGIN      
        1 cggacgccgc gctccgtttc ggccccgcgg agatgttctc gctgatgctc ttcgcgctcg
       61 tcgccaccat cacgctcgcc gaaggcagcc ttctgaaggg cgtcatcgca atgtgcatcg
      121 gcttcatgct ttcgacaatc ggcatcgacg cgctgaccgg catcctccgc ttcacctacg
      181 gcgtcgagga gctgcaggac gggatcgact ttcttgtggt gatgatcgga gtctacgcca
      241 tctgcgaggt cttcaagaac ctgaagaatc tggagacgaa gtacaacctc gactcgaaga
      301 gcatcggcaa ggtgtgggta agctggccgc agttcaagaa gtgcatcatg cccatgctcc
      361 gcagctctcc cctcggcttc ttcgtcggcg tgctcccggg aatgggcgcg agcatcgcca
      421 ccttcatgtc ctacgctatg gagaagaacc tgagcaagac gccggagaag ttcggcaagg
      481 gggccatcga aggactcgcg gcgcccgagg cggcgaacaa ctcctgcgcg gcgggcgcga
      541 tggtgccgct gctcacgctc ggcatccccg gctccggaac gacggcggtc atgctcggcg
      601 cgctgatgat gctcggcgtc aaaccgggac ccattctctt ctcccagtac ccggagatcg
      661 catggggcgt catcgcctcc atcctcatcg gcaacatcat cctcgtcatc atcaatctgc
      721 cgctggccat tccgctggtc cagctgctga aaattcctca gaggatcatg atgcccctca
      781 ttttgggtat ggccttcatg ggaacgtact ttctgaatta cagctccttc gatttcatcc
      841 tcgtcatggg gttcgccctc ggcgggtaca tcttctccaa actcgaaata ccgctgcctc
      901 cgatgatact ggctcttatc ctgggaggca ccacggaaca gtcgttccgg aactcgatgt
      961 cgctctcgga cggttcgctc ggcatcttct tctcgaaacc gatctccgtg acgctcatcg
     1021 cactcgcggg cctctcgctc gtctactctc tgctgaaaag aaggaagcat gcccaatgac
     1081 tacatttttg gaccgtcccc ggcggtccct gctttacata ccggggaaca atccgggaat
     1141 gatccagaac tgcgccatct acggttcgga tggagtcctc ctcgacctgg agg
//
LOCUS       DAOSWW010000969         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_111311_length_1193_cov_2.836555, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000969 DAOSWW010000000
VERSION     DAOSWW010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111311_length_1193_cov_2.836555"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PK442_14655"
     CDS             complement(<1..657)
                     /locus_tag="PK442_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HOO88944.1"
                     /translation="MDMLKSVIDVGTNSIKFYVARRRPDGSLEAVLDKNDIARLGEGL
                     RETGKISPEPMERNARSVAAFVEEARSLGVPSPEIVGTMALRTASNASDFVAKVKELT
                     GLTLRVIEGEEEARLSYLAVLSALPIGKGELVTFDTGGGSTEFVFGRGDEMLRKFSVP
                     LGAVRITEEFFKDDPVRQDSVDAAFAEIRKSLKEGGAGGSPDMLVGMGGTVTSLASVK
                     H"
     gene            complement(661..>1193)
                     /locus_tag="PK442_14660"
     CDS             complement(661..>1193)
                     /locus_tag="PK442_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88945.1"
                     /translation="EPEARIRQFEQGNYKESEEVYVLSRNSDENVKVRDDLLDIKSLQ
                     EVNADGLEQWFPVMKAGFPIPIDDLRKLFSYFKAPEPIFGRKEYTFDQFLNEIVRPNQ
                     NLRLVQVKKKRYIYVINGAVVEIAETDFDGVALRTICVEHEDPEVVMKTVRELALESL
                     PNINYIQAMKKTAGMA"
BASE COUNT          243 a          374 c          310 g          266 t
ORIGIN      
        1 gtgcttgacg gaggcaaggc ttgtcaccgt accccccata ccgacgagca tatccggcga
       61 accgcccgct ccaccctcct tcagactttt gcgaatctcg gcgaacgcag cgtctacgga
      121 gtcctggcga accggatcgt ccttgaaaaa ctcctcggtg atccggaccg ctcccagcgg
      181 cacgctgaac ttgcggagca tctcgtctcc cctgccgaaa acgaactcag tgctgcctcc
      241 gccggtatcg aaggtcacaa gctccccttt tcctatagga agagccgaaa gaaccgcaag
      301 gtaggaaagg cgcgcctcct cctccccttc gatcacacgg agcgtaagac cggtgagctc
      361 ctttaccttc gcgacgaagt cggaagcgtt ggaagcggtc cgcagcgcca ttgttcccac
      421 tatttcaggc gaaggtacgc cgagagagcg agcctcttcg acgaaagcag ccacggaacg
      481 ggcgttccgc tccatcggct ccggagaaat cttgcccgtc tcgcgaagac cttcgccgag
      541 gcgcgcgata tcgttcttgt cgagcaccgc ttccagcgaa ccgtcgggac gtctcctggc
      601 gacatagaac ttgatcgagt tggtgcctac gtcgatgacc gacttgagca tatccatcgc
      661 ctatgccatc cccgcggtct tcttcatcgc ctggatgtag ttgatgttcg gaagagattc
      721 cagagcaagc tcacgcaccg tcttcatgac gacttcagga tcttcgtgct cgacgcagat
      781 ggtgcgaagc gccactccgt cgaaatccgt ctccgctatc tcgacgacgg ccccgttgat
      841 gacgtaaata tagcgcttct tcttcacctg caccaggcgc agattctgat tggggcggac
      901 gatctcgttg aggaactggt cgaatgtgta ctcttttctg ccgaaaatgg gttccggggc
      961 tttaaagtag gagaaaagct tccggagatc gtctatcgga atcggaaatc cggccttcat
     1021 cacggggaac cactgctcca gaccgtctgc gttgacctcc tgaagcgact tgatgtccaa
     1081 tagatcatcc cgcaccttga cgttctcgtc gctgttccgg gagagcacgt agacctcttc
     1141 gctctcttta taatttccct gttcaaactg cctgatcctc gcctcgggct ctt
//
LOCUS       DAOSWW010000970         1192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_111426_length_1192_cov_3.547054, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000970 DAOSWW010000000
VERSION     DAOSWW010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111426_length_1192_cov_3.547054"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..259)
                     /locus_tag="PK442_14665"
     CDS             complement(65..259)
                     /locus_tag="PK442_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88946.1"
                     /translation="MLGQYYLITGIGAFALILLAVITGLFGRNLRKIVPGPLVLKIHK
                     FSALTGALCALLHVLGVHGY"
     gene            complement(311..754)
                     /locus_tag="PK442_14670"
     CDS             complement(311..754)
                     /locus_tag="PK442_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOO88947.1"
                     /translation="MIVSLHHASFTVENLERSVAFYQETLGLKLEGVWERDQEYSEGV
                     TGIRGAWLKVAYFSLPNASFELVEYLGAKGKKIDTSTNNVGSAHVCLIVANFDAFVEA
                     LAERGVVFAGKTCTIPAGSNKGKKIAYIEDPDGNTLELVSSRALS"
     gene            complement(751..>1192)
                     /locus_tag="PK442_14675"
     CDS             complement(751..>1192)
                     /locus_tag="PK442_14675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO88948.1"
                     /translation="AFMHRLDEESSRINRTNGRGSVILVDLGDFKQINDRYGHVTGDE
                     VLRVTSATMSENLRNYDMIGRYGGDEFLLYLPDVSREEAGAIMVRMELKVAEQKIEGF
                     EGAVILDYGIAGCPEDGINLSEVIRIADERMYRYKAERKAVKSE"
BASE COUNT          263 a          349 c          292 g          288 t
ORIGIN      
        1 tctttatggt aaactgtgac gccggagaat aaagacgcgt tgtgaagccg gcaggcggac
       61 gctgctaata cccgtgaacg ccgagcacgt gcagaagagc gcaaagagcg cctgtaaggg
      121 ccgagaactt gtggattttc agcacaaggg gcccggggac gatcttcctg aggtttctgc
      181 cgaacaaccc ggtgatcacg gcgaggagaa tcagagcaaa agcacctatc ccggtaatca
      241 aataatactg accaagcatg acgtttttat cctcctttgc tggttctttc cggttttcgc
      301 atccggcgtg ttacgaaaga gctctcgatg acaccagctc aagggtattt ccgtcggggt
      361 cctcgatata ggcgattttc tttcccttgt tcgaaccggc agggatagtg caggttttcc
      421 ctgcaaaaac gacgccgcgt tccgcaagcg cctcaacgaa ggcgtcgaaa tttgcaacaa
      481 taaggcacac atgagcgctt ccgacattat ttgtcgacgt gtcgatcttc ttccccttcg
      541 cccccaggta ctccacaagt tcaaaagaag cgttcggaag agaaaaatag gccactttga
      601 gccatgcccc gcgtatgccg gtgacgccct cggagtattc ctgatcccgc tcccacactc
      661 cttcgagttt cagccccagc gtttcttgat aaaacgcgac cgacctttcg agattctcca
      721 ccgtaaaact ggcatgatgc agcgatacga tcattcactc ttcaccgcct ttctctccgc
      781 cttgtatctg tacatccttt catccgcaat cctgatgact tccgagaggt ttattccatc
      841 ctcagggcag ccggctatgc cgtaatcaag gatgaccgct ccctcaaagc cttcgatttt
      901 ttgctcggcg accttcagct ccatccgtac catgatcgcc ccggcttcct ctcgtgagac
      961 gtccggcagg tagagaagga actcatctcc gccgtagcgc cctatcatat cgtaattcct
     1021 gaggttttca ctcattgtgg cggatgtcac acgaaggacc tcgtcccccg taacgtgccc
     1081 gtaacggtcg ttgatctgct tgaagtcgcc gaggtcgacg agaatcacgg agcctctgcc
     1141 gttcgtcctg ttgatgcggg aactctcttc gtcaagccgg tgcatgaagg ct
//
LOCUS       DAOSWW010000971         1191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_111651_length_1191_cov_2.110035, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000971 DAOSWW010000000
VERSION     DAOSWW010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111651_length_1191_cov_2.110035"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="PK442_14680"
     CDS             <1..1013
                     /locus_tag="PK442_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO88949.1"
                     /translation="ELELPSVPEYAPQEEDVRADPDFADIRGQAGARRALEIAAAGHH
                     NILLIGSPGSGKTLLARALRGILPPLSHEELLETTLVRSTLGLPFERGVQPPFRSVHH
                     TASTVSICGGGATLRPGEISLAHRGVLFLDEFPEFRRDLLEGLRQPLEDGAITVSRAA
                     GSALYPCRVLLVVAANPCQCGWDGDPVERCVCSHLERERYRRKISGPILDRIDLHVSV
                     PRLLPEELVAVESAGGECSADVAKRVRAAREIQRQRWAPHGFSCNAELPERLVKRSLA
                     LSAGVRPFLSSMAGSLRLSGRGIGRVLKVARTIADLARSESVEVPHVAEALAYRDGGV
                     WR"
     gene            1010..>1191
                     /locus_tag="PK442_14685"
     CDS             1010..>1191
                     /locus_tag="PK442_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88950.1"
                     /translation="MNPVLRTLLLLAGSGAPAVRIWNAFESEALPPEAFWEEGEVLWC
                     RLGCTEAVCRMLRRLA"
BASE COUNT          183 a          390 c          404 g          214 t
ORIGIN      
        1 gcgaattgga gcttccctcg gtgcccgagt acgcgcccca agaagaagat gtccgtgccg
       61 accccgactt cgccgatatc agggggcagg caggggcgcg ccgcgcactg gagatcgcgg
      121 ctgcggggca ccataacatc ttgctcatcg gttcgccggg aagcggaaag acgctcctcg
      181 cacgggcgct tcgcggcatc ctcccgccgc tctcccacga ggagctgctg gagacgaccc
      241 ttgtacggag cacgctcggc ctccctttcg aacggggagt gcagccgccg ttccggtcgg
      301 tccatcacac ggcgagcacc gtctccatct gcggcggcgg cgcgaccctt cgccccggag
      361 aaatcagtct cgcccacaga ggggtgctct tcctcgacga gtttcccgag ttccggcgcg
      421 atctgctcga aggactgcgt cagccgctgg aggacggggc gatcacggtc agcagagctg
      481 cgggatccgc gctctacccc tgccgcgtgc tgctcgtcgt tgccgcgaac ccctgtcagt
      541 gcggctggga cggcgatccc gtggaacgat gcgtctgctc tcacctggag agggagcgct
      601 accgccgcaa gatttccgga cctatcctcg acagaatcga cctgcacgtc tccgtgccgc
      661 ggctcttgcc ggaagagctc gtcgcggtgg agagcgcggg cggcgaatgc agcgcggacg
      721 tcgctaagcg tgtgcgtgcc gcacgggaga ttcagaggca acggtgggcg cctcacggtt
      781 tttcctgcaa cgccgaactt cccgagcgtc tggtcaaacg ctcgctcgcc ctttctgccg
      841 gcgtgcgccc cttcctctct tcgatggcgg gaagcctgcg cctctccgga agaggcatag
      901 gacgggtgct caaggtcgcg cggacgatcg cggatcttgc gcgaagcgaa agcgttgaag
      961 tcccccacgt cgcggaagcc ctcgcgtaca gggacggagg ggtctggaga tgaaccctgt
     1021 gctccggacg ctcctgctgc ttgcgggctc gggcgctcct gccgtgcgta tctggaacgc
     1081 attcgagtcg gaggctcttc ctcctgaggc gttctgggag gagggggaag tactctggtg
     1141 tcgccttggg tgtaccgaag ctgtgtgcag gatgctgcgt cgcctcgcgg a
//
LOCUS       DAOSWW010000972         1187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_112184_length_1187_cov_3.431095, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000972 DAOSWW010000000
VERSION     DAOSWW010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112184_length_1187_cov_3.431095"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..489)
                     /locus_tag="PK442_14690"
     CDS             complement(4..489)
                     /locus_tag="PK442_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88951.1"
                     /translation="MKINVRTPVILAVALLVALFVAGSVASASVQPVNFGQDHIKMQW
                     YLVNYGEENGVHFANARKYYTNPDVKAETIALIVEKFGTDQELANGLYFTEYGYAYSK
                     DGKSFAVTYINHYDMLGKVIRATEYDEASREFVGMSKDMIPFKAYAYATGKAKPAAKK
                     K"
     gene            complement(597..>1187)
                     /locus_tag="PK442_14695"
     CDS             complement(597..>1187)
                     /locus_tag="PK442_14695"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOO88952.1"
                     /translation="PHFGMTTPLVETRAAEAVLGGSRVEAVRTGGGNPIPDGKLVLAV
                     YGAASRFIESLHFGETVSLSLQLNEGDPAWSSMSHIVQGGPFLLRNGEVLSEREKLSD
                     SIIDRRHPRTVVGLTKEGHWFFFVGDGRNAVHSVGFTLDEVSRILKSAGVSYALNLDG
                     GGSSGIISSGGVWNIPSDGLERPVSYGIGAFQRRGR"
BASE COUNT          248 a          351 c          323 g          265 t
ORIGIN      
        1 cagctacttc ttctttgcgg cgggcttcgc ttttcctgtg gcgtaggcgt acgccttgaa
       61 gggaatcatg tccttggaca ttccgacgaa ttctcgtgaa gcctcgtcgt actcggtggc
      121 gcggatgact tttcccaaca tgtcgtagtg attgatgtac gtcacggcga aagacttgcc
      181 gtccttcgaa taggcgtagc cgtactcggt gaagtacaat ccgttggcaa gctcctggtc
      241 ggtgccgaac ttctcgacga tgagcgcgat ggtctcggcc ttgacgtccg gattggtata
      301 gtactttctg gcgttcgcga agtggacgcc gttctcttcg ccgtagttca cgaggtacca
      361 ctgcatcttt atgtggtcct gaccgaagtt gaccggctgc accgaggcgg atgcaacgga
      421 accggcgaca aagagtgcga caagaagagc gactgccaaa ataacgggtg tacgtacatt
      481 gattttcatg ctgaatgaga caactccttc caatttttca tctgccttac gccgtgtagt
      541 ctaccacaaa acggcgaaaa taaaacgccc ggggagcatt ttcttcgttc ctttctctac
      601 cgcccccttc tttgaaaggc tccgatgccg taactcaccg gacgctccaa gccatcggaa
      661 gggatattcc agaccccccc gctggagata atccccgaag aaccgcctcc gtcgaggttc
      721 agcgcatagg aaaccccggc gctcttcagg atgcgggaga cctcgtcgag cgtgaagcct
      781 acgctgtgca cagcatttct tccgtcgcct acgaagaaaa accaatgccc ctctttcgtc
      841 agcccgacga cagtcctggg gtgacggcga tcgataatcg agtcgctcaa tttttctctc
      901 tcggagagga cttccccgtt ccggagaagg aaagggcctc cctgtacgat atgcgacatt
      961 gaactccacg ccggatcccc ctcgttcagc tgaagcgaga ggctgaccgt ttcgccgaaa
     1021 tgcagagact cgataaagcg cgacgccgcc ccgtagactg cgagaacgag cttcccgtcg
     1081 ggtatcgggt ttcctccccc tgtacggacc gcctccacgc gcgagcctcc gagaacagcc
     1141 tcggccgcgc gggtttcgac aagaggggtg gtcatgccga agtgcgg
//
LOCUS       DAOSWW010000973         1187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_112312_length_1187_cov_1.190813, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000973 DAOSWW010000000
VERSION     DAOSWW010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112312_length_1187_cov_1.190813"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PK442_14700"
     CDS             <1..254
                     /locus_tag="PK442_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HOO88953.1"
                     /translation="DLPDILARWQHKDKETDRKRTEQSFLVPKSEIAANDYDLSINRY
                     KEVTYEAVEYDPPKVILERLAALEAEIAEGRRELEGLLG"
     gene            251..>1187
                     /locus_tag="PK442_14705"
     CDS             251..>1187
                     /locus_tag="PK442_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HOO88954.1"
                     /translation="MIRLKVFISSVQKEMRSERFAIGAFLSTDDFLRECTVPRIFEEY
                     PQPLFPNPKAYLDLLRQCQIYLLLVGSQYGIDAGDGLSATHEEYRLAQELKLPTLICI
                     KGARRVKREQAVNAFLKEIETAKHTYSRFENEAQLLDIVGKRLREHIEITYSTVPLKA
                     QIEQSALTHQSASEFERGMLDAMTYEDLDPELMLDLTASAEERDKERILPDEIPRLLL
                     SRGYLWKDVDTLRPTVAGALLLARKPGMALPQARVQMDAFPGTDRNAEALDSEIFDAP
                     LSKAVERAVAFIRRNTPKPLIVKGLKRVKAETYPAE"
BASE COUNT          278 a          362 c          337 g          210 t
ORIGIN      
        1 gcgacttgcc cgacatcctc gcccgctggc agcacaagga taaagaaacc gaccgcaaac
       61 gcacagagca gagcttccta gtccccaaaa gcgaaattgc cgccaacgac tacgacctat
      121 ccatcaaccg ctacaaggaa gtgacctatg aggcggtgga gtacgacccg cccaaggtga
      181 tcctcgaacg cctcgccgcc ctggaggccg agatcgccga gggacgccgc gagctggagg
      241 ggttgctggg atgattcgtc tgaaagtgtt catcagctcc gttcaaaaag agatgcggtc
      301 cgaacggttc gccatcgggg ctttcctctc gaccgacgat tttctgagag aatgcacagt
      361 accgcgcatt ttcgaggagt atccgcaacc gctctttccc aatcccaagg cgtatttgga
      421 cttgcttcgc cagtgccaga tctatctgtt gcttgtcggg tcccagtacg gcatcgacgc
      481 cggagacgga ctctccgcga cgcacgaaga gtaccggctc gcgcaggagt tgaagctgcc
      541 gacgctgatc tgcatcaagg gagcgcgacg ggtgaagcgg gagcaggccg tgaacgcttt
      601 cctcaaggag atcgaaacgg cgaagcacac ctacagccgc ttcgagaacg aagcgcagct
      661 gctcgacatc gttggtaaac gcctgcggga acatatcgag ataacctatt ccaccgttcc
      721 tctgaaagcg cagatagagc aatccgccct gacgcatcag tccgcctcgg agtttgaacg
      781 gggaatgctt gacgcgatga cgtacgaaga cctggacccc gaactcatgc tggatttaac
      841 ggcgtcggcg gaagaacgcg acaaggagcg catccttccc gacgaaattc cacgactgct
      901 cctgtcccgc ggctatctct ggaaagacgt cgataccttg cgcccgaccg tcgccggagc
      961 gttgctcctg gcgcgcaagc cgggaatggc gcttcctcag gctcgcgtac agatggacgc
     1021 cttccctgga acagaccgca acgcggaggc gcttgattcc gaaatcttcg acgcgccgct
     1081 ctccaaggca gtcgaaagag ccgtcgcctt catccgcaga aacactccaa agcctttgat
     1141 cgtgaaagga ttgaaacgag tcaaggcgga aacgtatccc gccgagg
//
LOCUS       DAOSWW010000974         1186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_112339_length_1186_cov_3.389036, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000974 DAOSWW010000000
VERSION     DAOSWW010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112339_length_1186_cov_3.389036"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PK442_14710"
     CDS             complement(<1..738)
                     /locus_tag="PK442_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO88955.1"
                     /translation="MFCLPWEREGRCLSDVELLETGRGILHAEAAEIERAAQRTGLEL
                     VKAARIVHSCTGRLVVVGMGKSGIIGRKIAATLASLGTPSFFLHAAEGSHGDLGMVCR
                     DDAALFISNSGTTAEVVSLLPHFKRLGAPIIAITGGLNSPLAKNADAVLDSSVRSEAG
                     ITACATPLEPGERDQDALNLAPLCSTTLQLALGDALAGMVTELRGLRPEDFALFHPGG
                     ALGRRLLTRVGDVMGTGEKLPLVGMNVS"
     gene            complement(740..>1186)
                     /locus_tag="PK442_14715"
     CDS             complement(740..>1186)
                     /locus_tag="PK442_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOO88956.1"
                     /translation="MKHVVEKCRSGGCSGLMLCERGTFMGYGQLVVDMRSLPLMRALG
                     CPVVFDATHSVQMPGAAGGASGGDRRFVLPLARSAVAAGIDALFLETHPRPEKAKSDG
                     PNLVPLGKMRFLLEQLAELHAFVRERFGRLSLEWADEHAAENKGGR"
BASE COUNT          195 a          403 c          398 g          190 t
ORIGIN      
        1 cgagacgttc atgccgacaa gcggcagctt ctcccccgtc cccatgacgt cgcccactct
       61 ggtgagcaac ctgcggccca gggcgccccc cggatggaag agggcgaagt cctccggccg
      121 gaggccccga agctccgtca ccatccctgc gagcgcgtcg ccgagtgcga gctgcaacgt
      181 cgtgctgcac aagggggcga ggttgagcgc gtcctgatcg cgttctcccg gttcgagggg
      241 cgtcgcgcac gcggtgatgc ccgcctcgga acgcaccgag gagtcgagca cggcgtcggc
      301 gtttttcgcc agaggggagt tcagtcctcc ggtgatggcg atgatcggcg cgccgaggcg
      361 tttgaagtgc gggagcagcg agacgacctc cgccgtggtt ccgctgttgc tgatgaagag
      421 cgccgcgtcg tcgcggcaga ccatgccgag gtcgccgtgc gacccttcgg cggcgtggag
      481 gaagaaggag ggagtaccca gggaggcgag cgtcgccgcg atcttccgcc cgatgatccc
      541 cgacttgccc atgcccacta ccacgagcct gccggtacag gagtgcacga tccgcgcggc
      601 cttcaccagc tccaagcccg ttcgctgcgc ggccctttct atctcggccg cctcggcgtg
      661 cagaatccct ctccccgttt ccaggagttc aacgtcggag agacatcgtc cctcgcgctc
      721 ccacggaagg cagaacatct cagcgtcccc ctttgttttc ggctgcgtgt tcgtcggccc
      781 actccaggga gagacggccg aatcgttcgc ggacgaaagc gtgcagttcg gcgagctgtt
      841 cgaggagaaa gcgcatcttc cccaggggaa cgaggttcgg accgtcgctc ttcgccttct
      901 cggggcgggg gtgcgtctcc agaaagagcg cgtcgatacc ggcagccacg gcgctccggg
      961 caagagggag gacaaaacgt ctgtcgcccc cgctcgcgcc gcccgcggcg ccgggcatct
     1021 ggacgctgtg cgtggcgtcg aagacgacag ggcatccaag cgcgcgcatg aggggcaggg
     1081 agcgcatatc gaccacgagc tggccgtacc ccatgaaagt gccgcgctcg cagagcatca
     1141 ggccggagca gcctccgctg cggcacttct ccaccacgtg cttcat
//
LOCUS       DAOSWW010000975         1182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_112970_length_1182_cov_4.043478, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000975 DAOSWW010000000
VERSION     DAOSWW010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112970_length_1182_cov_4.043478"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK442_14720"
     CDS             complement(<1..214)
                     /locus_tag="PK442_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88957.1"
                     /translation="MTGFRRIVCALFAVCCVLSLPAVAAEDASARFDELAARYFVRME
                     GREDALALRAALEAEAVQADEFALWRS"
     gene            482..>1182
                     /locus_tag="PK442_14725"
     CDS             482..>1182
                     /locus_tag="PK442_14725"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOO88958.1"
                     /translation="MNIQRADSLSELVERVRSLEPFLDSLGDIVSLVDPGMRYLWASA
                     SMEASLALRRSEIVGGECFRLHHAVSSVCPHCPVREAMSTGQVVEKELYSKERKKHFL
                     TRGIPVFGPDGRVIAAFEVTRDITDMKTRHLETEAKERYFRYLFENAPVGIFQISMDG
                     KYIAANARQALILGYESPEELMEVVNRTSVKEAHYSSSESREAVLKNLMEHPNLWTTR
                     ETEFRRRDGLLIDVQL"
BASE COUNT          265 a          364 c          305 g          248 t
ORIGIN      
        1 gggagcgcca cagagcaaac tcgtcggcct gcacggcctc cgcttccagg gcggcccgga
       61 gcgccaacgc atcctcccgt ccttccatcc tcacaaagta gcgcgccgcc agctcgtcga
      121 aacgcgccga cgcatcctcc gcggcgaccg caggcaagga gagaacgcag cacactgcaa
      181 aaagagcgca gactatgcgc cgaaaacccg tcatgagcat caactccttc agctcctttg
      241 gagacatcga attatcttag gtgcttcgtc cattctacca ctcaaaatta gtcagtaatg
      301 atcaatcatt atatactctt ctttcgcgtc tgaaaacccg gcggagcgca aaaaacccgc
      361 cgtgaagcgg aaaactcccc gatggtttca cttttcgcgc gcaatgatca catccctctc
      421 tctttcccgt gctctatcct gtagaatata cgtatgatgc gggaaaggga gcgatagagg
      481 aatgaacatt caacgagcag actctctcag cgaactggtc gaacgggtac gttccctgga
      541 gccgtttctc gactccctcg gggatatcgt ctcccttgtc gatccaggga tgcgctacct
      601 ctgggcatcc gcttcgatgg aagcctctct cgctctccgc cgttcggaga tcgtgggggg
      661 ggagtgtttc cgccttcatc acgccgtttc ctccgtctgc ccgcactgcc ccgtccgcga
      721 ggccatgtcg accgggcagg tcgtcgaaaa agagctgtac tccaaagaac gaaagaaaca
      781 ctttttaacc agggggattc ccgtcttcgg tcctgacggt cgcgtgattg cggcctttga
      841 agtcacccgc gatatcaccg atatgaagac tcgtcacctt gagacggagg cgaaggagcg
      901 ctatttccgc tacctcttcg agaacgcacc cgtcgggatc ttccaaattt ccatggacgg
      961 taaatatatc gcggcgaacg cgcgccaggc gctcatcctt gggtatgagt ctccggagga
     1021 gctcatggag gtcgtcaaca gaacatccgt caaggaagcg cattactctt cctccgagtc
     1081 cagggaagcc gttctgaaaa atctcatgga gcatccgaac ctgtggacca cccgggagac
     1141 cgagtttcgc agacgcgacg gcttgttgat cgacgtccaa ct
//
LOCUS       DAOSWW010000976         1181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_113149_length_1181_cov_4.023091, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000976 DAOSWW010000000
VERSION     DAOSWW010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113149_length_1181_cov_4.023091"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PK442_14730"
     CDS             <1..222
                     /locus_tag="PK442_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88959.1"
                     /translation="APVRPTAIILYGDPVPVKPRLSWRISTFCLPTAFLNAGTFPAPA
                     FWVYSTARGEISPGDPGKWYTFTPERILI"
     gene            complement(521..721)
                     /locus_tag="PK442_14735"
     CDS             complement(521..721)
                     /locus_tag="PK442_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88960.1"
                     /translation="MIPRQYCTVPVHIRMNVFRCIQDALFFVEKNQVAVTIHPFENQK
                     IRDSEPGADKAFGFQQQNAVVV"
     gene            752..>1181
                     /locus_tag="PK442_14740"
     CDS             752..>1181
                     /locus_tag="PK442_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88961.1"
                     /translation="MWSTKREHLSLLDIRTLIGIGRPLVKTIIEHWESFPLSFWSEAI
                     DGLLGGAPSALPLGLQRIGNYITVFPVLFKDAEALDGSPIERCGKETAGKLLSLHREK
                     GLASVRKAIAKEIRSRERHADGLRKQLSLSGHAEEFKKYGE"
BASE COUNT          295 a          303 c          304 g          279 t
ORIGIN      
        1 gccccggtca gaccaacggc gattatttta tacggcgatc ccgttcctgt caagcctcga
       61 ctttcgtgga gaatttcgac tttctgttta ccgacagcat ttttgaatgc cggaaccttt
      121 cctgcaccgg ccttctgggt atattccact gccagaggcg aaatttctcc tggagatccg
      181 gggaagtggt atacttttac ccctgaaaga attttgatct gaaaacggag tgatgctcgt
      241 gtctttcggt ccggaactca ttacggttct tgcgcgtgaa gtcaatgcac gcgaaatgcc
      301 ggcgcgaata cataagattg aaacgggcga tgtgtgggcc gcactgaaga taggcggtga
      361 aaaatggctc ttcttttcgt ggcatgctgt tcattacgga ctgggtatcg tcggggaaga
      421 aactgttgct gcactgaaga aaatgaagac gcccaagtcg tcattcggcg aagccctgaa
      481 aagaaacttt ctcaatggaa cagttctttc ggcaaaacaa ctatacaacg accgcattct
      541 gctgctggaa accgaacgcc ttgtcggcgc cgggttcact gtctcgaatt ttttgatttt
      601 cgaagggatg gatcgtaaca gcaacctgat tcttctcgac gaaaaacagt gcgtcctgga
      661 tgcagcgaaa cacattcatc cggatgtgaa ccggtaccgt acagtattgc cggggaatca
      721 ctacacgccc cctcccccgc gggacggggc tgtgtggagt acgaaaagag agcacctttc
      781 ccttctcgat atacgtaccc tcatcggtat cggcagacct cttgttaaaa ccataattga
      841 gcattgggag agcttccctc tctccttttg gtctgaggca atcgacggat tgcttggcgg
      901 cgcgccgagc gcacttcccc tcggtctcca gaggatcggt aattatataa cagtcttccc
      961 ggtgcttttc aaagacgcag aagcactgga tggaagtccg attgaacgct gcgggaaaga
     1021 aaccgctggg aagctcctct cgctacaccg cgaaaaaggc ctcgcatccg tacgcaaagc
     1081 aatagcaaaa gagatacgaa gccgtgaacg ccatgctgac ggattacgca aacagctctc
     1141 actttccggg cacgccgaag agtttaaaaa gtacggagag c
//
LOCUS       DAOSWW010000977         1178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_113626_length_1178_cov_3.816563, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000977 DAOSWW010000000
VERSION     DAOSWW010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113626_length_1178_cov_3.816563"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PK442_14745"
     CDS             complement(<1..1038)
                     /locus_tag="PK442_14745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO88962.1"
                     /translation="MAKKRFQHELELRQFTVDDYDEVKRIMDRIYPKMGGAWTKEQFS
                     SMISRFPEGQLCIEDKGRVVAGALSLIIDYSRYGDRHTYRDIVGDGYLKNHDPEGDYL
                     YGIDIFVSPDYREKRLGRRLYDARKELCETLNLKGIIIGGRIPGYAEWAEKKTPQQYI
                     RMVNNREISDPVLTFQVSNEFHYKKIIENYWPEDYSSKGYGVILEWNNIYYEERQRLI
                     SKVKTYMRLGVVQWQMRSFESFEDFLQQVEFFVDTQAGYNTDLMLFPELFNAPLLAQF
                     DQEDPAEAMRLLAGYTADLRRNLVEMAVAYNINIVSGSVPELRNNRLYNVTFLCRRDG
                     TWDQQYKLHITP"
BASE COUNT          237 a          322 c          322 g          297 t
ORIGIN      
        1 gggggtgatg tggagcttgt actgctggtc ccatgtgccg tcgcggcggc aaagaaaagt
       61 gacgttgtag agcctgttgt tccggagctc cggcacgctt cccgagacga tgttgatgtt
      121 gtaggccacc gccatctcca cgagattcct gcgaagatcg gccgtatacc ctgcgagcag
      181 gcgcatggcc tccgcgggat cttcctggtc gaactgcgcg agcagcggcg cgttgaacag
      241 ctcggggaag agcataaggt cggtgttgta gccggcctgg gtatcgacga aaaattccac
      301 ctgctgcaga aaatcctcga aggactcgaa gctccgcatc tgccactgga ccacgccgag
      361 gcgcatgtag gtcttcactt tggaaatgag gcgctgccgc tcctcgtagt agatattgtt
      421 ccattcgaga ataacgccgt accccttcga gctgtagtcc tcgggccagt agttctctat
      481 gatcttcttg tagtggaatt cattgctcac ctgaaaggtc agcaccggat cggagatctc
      541 ccggttgttc accatacgga tatactgctg cggcgttttc ttctccgccc actcggcgta
      601 cccggggatt ctgccgccga tgatgatccc cttcagattg agcgtctcgc acagctcttt
      661 gcgggcatcg tagaggcgac ggcccagccg tttctccctg tagtccggag agacgaagat
      721 atcgataccg tagaggtagt ctccttccgg atcatggttt ttcaggtatc cgtcaccgac
      781 gatatccctg tacgtatgcc ggtcgccgta gcgggagtag tcgatgatga gagagagcgc
      841 gcctgcaacg acccgtcctt tgtcctctat gcagagctgc ccctcgggaa agcgagagat
      901 catggaagaa aactgttctt ttgtccaggc gccccccatc ttggggtata ttctgtccat
      961 gatgcgcttg acttcgtcgt aatcatcaac ggtgaactgg cggagttcaa gctcatgttg
     1021 gaagcgtttt tttgccattg acttttcatt cctttcaatt tccgggtttt cctattatac
     1081 gttgacgacg gacaaaaaac atctgtaaac ggaagaagcg aggtcgggct cttccattca
     1141 cccttcgtgt ccctctctct tcagagaatc agatctca
//
LOCUS       DAOSWW010000978         1177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_113822_length_1177_cov_2.741533, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000978 DAOSWW010000000
VERSION     DAOSWW010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113822_length_1177_cov_2.741533"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..720)
                     /locus_tag="PK442_14750"
     CDS             complement(190..720)
                     /locus_tag="PK442_14750"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOO88963.1"
                     /translation="MMVKTLTGANDSPEIRERLVLKALRVSLGHLNGCVLMIELNEAV
                     MANDHLYYALFHAAKAALFARGKDPSTHQGVKMELSGVASENGLDREFIRFFGNRERL
                     RSEMSYSYLGEVETFSIQEVTTALQASHRWMKTLIDELVRPLIPSVPEFDELVRPSET
                     GNSLLGDKGLWSVLHE"
     gene            complement(717..>1177)
                     /locus_tag="PK442_14755"
     CDS             complement(717..>1177)
                     /locus_tag="PK442_14755"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO88964.1"
                     /translation="EGGGAMPLMTNSSPVCSSAAMDSSWTKRRNNAIRRRCEQFACSL
                     ETLFGDDLLAVWLYGSCATKTNHAESDVDVAIILTEAGKKRYEQNTDGIASDCDDLEF
                     SMARELAMPVSLLIWSEERLSREIAFCTGFASRLLEKNVVLFQKRGWMPK"
BASE COUNT          257 a          340 c          312 g          268 t
ORIGIN      
        1 ccggagattt tgacggcgtt tcgcccgagg gcggacaact cccgagggcg aagcgcctac
       61 ggcatatttt gatagaggtt gatcccgacg cggaaagcga gccgcaaaga ctcttcatac
      121 gctgtttttc tccgtccgga cgctcttttt gaagtctgtc gctgttgcct cctccagggc
      181 aacccgccgt cactcgtgaa gaaccgacca gagtcctttg tcgccgagaa gggagttccc
      241 tgtttccgaa ggtcgcacaa gttcgtcgaa ttccggaacg gaaggaatca gcgggcgaac
      301 aagttcatcg atcagcgtct tcatccagcg gtgggaggct tgcagggcag tggttacctc
      361 ctggatggaa aaggtctcaa cttcgcctag ataagagtag ctcatctccg agcgcaatcg
      421 ttcacgattt ccgaaaaaac ggatgaactc acggtccaat ccgttttcgg acgctacccc
      481 ggagagttcc atcttcaccc cttgatgcgt ggaaggatcc ttgcctcttg caaaaagcgc
      541 tgctttcgcc gcatgaaaca gcgcgtagta gaggtgatcg ttcgccatca cagcctcgtt
      601 cagttcgatc atgagcacgc acccgttcaa atggccgaga gagacgcgga gggccttgag
      661 aaccagccgc tccctgattt caggagaatc gttcgctccc gtgagcgttt tcaccatcat
      721 ttcggcatcc acccccgttt ttggaacagc acaacgtttt tttccagcaa acgagaggcg
      781 aagcccgtgc aaaaagcgat ctcgcgcgaa agcctctctt cgctccatat cagaagcgag
      841 acgggcatcg cgagttctct cgccattgaa aattcgagat catcgcagtc ggaggcgata
      901 ccatcggtat tctgctcgta gcgtttcttt cccgcctcgg tcaggataat tgcaacgtcc
      961 acgtcggatt ccgcatgatt cgtcttcgtc gcacaggagc cgtacaacca gacggcgagc
     1021 agatcgtccc cgaaaagggt ttcaagtgag caggcgaatt gttcgcaccg acgacggatt
     1081 gcgttgtttc ttcttttcgt ccacgacgag tccatcgcgg cggatgaaca cacaggcgag
     1141 ctgtttgtca tgagcggcat tgctccgccc ccttcca
//
LOCUS       DAOSWW010000979         1177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_113837_length_1177_cov_2.529412, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000979 DAOSWW010000000
VERSION     DAOSWW010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113837_length_1177_cov_2.529412"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PK442_14760"
     CDS             complement(<1..1007)
                     /locus_tag="PK442_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOO88965.1"
                     /translation="MRKFFVALLAIVLLFGGAYGAWAADDFTILSFSPGGMVKGRMPV
                     KIAFSQPVVEKSLVGKTAPQQPIPVEFSPSIRGAGKWTDQKTFVFTPVANLQQATRYR
                     ATAKESLQDAKGRRLSGKQSFEFFTEPLKLLRAQQIDFTESNNVVIELKFNLPVSPFR
                     LRGFLSLLNEQRQQLRYSPAGQAPSSTVRLTTNSFEGGKIALVLSPGLTSDAGPLGIE
                     QEIRHEIAVTRKLEIRSVYADTRYPATSVINVNTSVGADMSVVSKFIEISPATEFTID
                     PGYNGFTILGDFKPKQRVELTIKKGLPTKEGKALEQEFKRAVIFPDYYPSLAFPAGGT
                     FL"
BASE COUNT          235 a          318 c          351 g          273 t
ORIGIN      
        1 aggaaggtcc ctccggccgg gaaggcgagg gacgggtagt agtcggggaa aatgaccgcc
       61 ctcttgaact cctgttcgag cgccttgccc tctttggtgg gaagtccctt tttgatggtc
      121 aattccactc gctgtttggg cttgaagtcg ccgaggatcg tgaagccgtt gtaccccggg
      181 tcgatggtga actccgtcgc gggcgagatt tcgatgaact tggagacgac cgacatatcc
      241 gcgcccacgg aggtgttcac attgatgacg cttgtcgcgg gataccgtgt gtccgcgtag
      301 acgctgcgga tctccagctt tctggtgacc gcgatctcgt gccggatttc ctgttctatt
      361 cccagaggac cggcgtcgct tgtcagccct ggagagagga cgagcgcgat tttcccccct
      421 tcgaaggagt ttgttgtcag gcggactgtt gatgaaggag cctgtccggc gggggagtat
      481 ctcagctgct gccgctgttc gttcagcagc gagagaaaac cgcgcagcct gaacggcgag
      541 acggggaggt tgaacttcag ttcgatcacg acattgttgc tctcggtgaa gtcgatctgt
      601 tgcgccctca ggagcttcag cggttcggtg aagaattcga agctctgctt tccggagagc
      661 cgccgtccct tcgcgtcctg cagcgactcc ttggcggtcg cgcggtatcg cgtcgcctgc
      721 tggagattcg ccacgggagt gaagacgaac gtcttctgat ccgtccattt gcccgctccg
      781 cgtatggacg gtgaaaactc aaccggtata ggctgctgcg gcgcagtctt gccgacgaga
      841 ctcttctcca cgactggctg cgagaaggcg atcttcaccg gcatcctgcc tttgaccatt
      901 cctcccggag aaaacgagag aatggtgaaa tcatctgccg cccaagcacc gtacgctccg
      961 ccgaagagca agacaatcgc gagaagcgcc acaaaaaact ttctcatatg tatcatctcc
     1021 tttttctctg gtctatatgg tagtgcgccg tgcatgtcgt tgctttttga attatacctc
     1081 gaatcgcggt gaagagggta tgcgaaagag acgaatacga aatacacgcg atcctctata
     1141 gaaaaacgga gagaagacat tctgtgccgg gctgttg
//
LOCUS       DAOSWW010000980         1171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_114777_length_1171_cov_4.076165, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000980 DAOSWW010000000
VERSION     DAOSWW010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114777_length_1171_cov_4.076165"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PK442_14765"
     CDS             complement(<1..845)
                     /locus_tag="PK442_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88966.1"
                     /translation="MKGKKKSHVKATLKDAPTETTEKGERRKIDEHWKAIIKKFFLLV
                     LRRVLPELADDLDTSRKVVFMEKELEELTVYVDGPKQIPDILARVPTASGRDVWLILH
                     VEIQGPEGGDLPERMFFYNSSLRVTHLKKRSDVTDAVSCAILTAKRPCGEEEMYLRES
                     YRNRVSYEYPSLKLWELDAGELESSNNPFDWALYAGKCALESDRSETLKLDYLTRLIE
                     KLDAKGWTHDEKLALFRFMDALLHPKSPELRKAYDDFREQRRKEGKIVLLSVMEERAQ
                     ERGKK"
BASE COUNT          217 a          370 c          289 g          295 t
ORIGIN      
        1 atcttcttgc ctctctcctg cgctctttcc tccatcacgc tcaacaacac gatcttcccc
       61 tccttccttc gctgttctct gaaatcgtcg tacgccttcc gcagttccgg gctcttcgga
      121 tgcagcaacg cgtccatgaa gcggaagagc gcaagctttt cgtcgtgtgt ccaccccttc
      181 gcgtcgagtt tctcgatcag cctcgtcagg taatcaagtt tgagcgtttc gctccggtcg
      241 ctctcaagcg cgcactttcc ggcgtagagc gcccaatcaa acgggttgtt gctgctctca
      301 agctcgcctg cgtccagctc ccacagcttc agcgacggat actcgtacga aacccggttc
      361 ctgtagctct cccgcaggta catctcctct tcaccgcagg gccgtttcgc cgtcagtatt
      421 gcgcaggata ccgcatccgt aacgtcgctc cgcttcttga ggtgcgtcac ccgaagagag
      481 gagttgtaga aaaacatccg ctccggcagg tcgcccccct caggcccctg tatttccacg
      541 tggaggatca gccacacgtc gcgccccgag gcggtcggaa cgcgggcgag aatgtcgggt
      601 atctgcttcg gcccgtcgac gtacacagta agctcctcaa gctccttctc catgaagacc
      661 accttccgcg aggtgtccag atcgtcggcc agctcgggaa gaaccctccg aaggacgagg
      721 agaaaaaact tctttatgat cgccttccag tgttcgtcga tcttcctgcg ttcccctttt
      781 tcagtcgtct ccgtcggggc gtcctttagc gtcgccttga cgtggctctt ctttttcccc
      841 ttcatttcgg cggacccctt caaatcgcgc acaatgacgt tcggccccct tcattttttc
      901 ggcggccgga agctccgccg aggttattgc gcgtcttgtt gtacgagaag agtatagctt
      961 catacaagat cttcttcaag cccaagatat tttaaagaaa tgctgagacc ggtgcgcatg
     1021 ttgaaaggaa gcactaggtt ccccgcaaag agaagtggcg attttcctct acatcttctc
     1081 gaaaagcgtg attaaacggt ccgtctggtc cgccatgggg cactcctgga tctcgtcgaa
     1141 aacgatgagg gtgtcttccg ggtcgatctt c
//
LOCUS       DAOSWW010000981         1168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_115229_length_1168_cov_3.414196, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000981 DAOSWW010000000
VERSION     DAOSWW010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115229_length_1168_cov_3.414196"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1168
                     /locus_tag="PK442_14770"
     CDS             <1..>1168
                     /locus_tag="PK442_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88967.1"
                     /translation="RDISVDKALGGVLVADFGGGTCDFAFLEGGRVVRSWGDMLLGGR
                     LFDDLFFQWLCDQNPGLDTELEAQGSAYFVQSHLCREVKEFFSRSMARDRGEPVSKVL
                     AGYGRLTDLTWEEFLRRGASYTPSRPFIEFSKGILPELRGRARSTAWNFLAHNGEEPL
                     QGQKLDLFGWFRECLRSGLEEGKIERERISLVILAGGSSLWPFVGEILREEIPLREER
                     ALVRSDRPYAVIAQGISLLPALKKRFARARQSLQNELPDFLENRLGALTANRVAALEE
                     KMASLAVDIFFRKEVVPLLLEFRKQGGSLALLRKRIDERTAAAEPLAREHLARLAPRF
                     LEGLREEVRDLLAEWFASHGVALPRKDLAFVLPDELSEPMLRVRTPAADGMLDVV"
BASE COUNT          176 a          394 c          391 g          207 t
ORIGIN      
        1 gcgcgacatc tcggtggaca aggcgctcgg cggcgtgctc gtcgcggact tcgggggcgg
       61 gacgtgcgac tttgccttcc tcgagggggg gcgcgtcgtc cggtcgtggg gggatatgct
      121 gctcggcgga cggctcttcg acgatctctt cttccagtgg ctgtgcgacc agaatccggg
      181 gctcgacacc gaactggagg cgcagggatc ggcctacttc gtccagtccc acctctgccg
      241 ggaggtcaag gagttctttt cgcgctcgat ggcgcgcgac cggggcgagc cggtatcgaa
      301 agtgctcgcc gggtacggca ggctgaccga cctgacctgg gaggagtttc ttcgcagagg
      361 cgcctcctac accccatcgc gccccttcat cgaattctcc aaggggattc tccccgagct
      421 gcgcgggcgc gcccgttcga cggcgtggaa cttcctcgcg cacaacggag aggagccgct
      481 ccaagggcag aaactcgacc tcttcggctg gttccgcgag tgcctccgct ccggccttga
      541 agaggggaag atagaacgcg agcgaatttc gctggtcatt ctggccggcg gaagcagcct
      601 ctggccgttc gtcggagaga tcctgcgcga agaaatcccc ctgcgggagg agcgcgccct
      661 ggtgcgcagc gaccggccgt acgccgtgat cgcccaggga atttcgctgc ttcccgcgct
      721 caaaaagcgc ttcgcccgcg cgcgccaatc gctccagaac gaactccccg acttcctgga
      781 gaaccgcctg ggcgcgctga cggcgaaccg cgttgcagcg ctggaggaga agatggcctc
      841 gctcgccgtc gacatcttct tccgcaagga ggtcgtcccc cttctgctgg agttccggaa
      901 gcagggcggc tcgctggcac tcctccggaa gcgcatcgac gagcggaccg ccgccgccga
      961 gcctctcgcc cgcgaacatc tcgcccggct cgctccgcgc tttcttgaag ggcttcggga
     1021 ggaggtacgc gacctgctcg cggaatggtt cgcgtcgcac ggcgtcgccc ttccgcggaa
     1081 agacctcgcg ttcgttctcc cggacgaact ctcggagcct atgctccggg tgcggactcc
     1141 cgcggcggac ggtatgctcg acgtcgtc
//
LOCUS       DAOSWW010000982         1166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_115530_length_1166_cov_3.846985, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000982 DAOSWW010000000
VERSION     DAOSWW010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115530_length_1166_cov_3.846985"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PK442_14775"
     CDS             complement(<1..409)
                     /locus_tag="PK442_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOO88968.1"
                     /translation="MKNDLRLSCIAALADTHLTGPDGPIPLRFVADSRLVVPGDAFVA
                     FRGEQVNGHDFIHDAVSRGASLIICEESAGVPNHVASIVVDNCFSAIPRMARRYLEIH
                     RESLEVLAVTGSVGKTTTREMIRTCLAPSFKVHS"
     gene            complement(406..888)
                     /locus_tag="PK442_14780"
     CDS             complement(406..888)
                     /locus_tag="PK442_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOO88969.1"
                     /translation="MERYSFNSGVSCIIDFAHTPFALQNVLTEVRRLCGGRIISVFGH
                     GGERFQPNRFSLGNIAATLADEVIVTMDNPRNEPAEQIAAQIVEGVESSERRVPLKTI
                     LDRSAAVYEAMDSAKPGDVVVVSGKGPEKYLRIGSESIPYSDGDTLRRWADERGVEWR
                     "
BASE COUNT          295 a          304 c          299 g          268 t
ORIGIN      
        1 ctgaatgtac tttaaacgag ggagccaggc aggtgcggat catctcacgc gtcgttgttt
       61 tcccgacact tccggtgacg gcaagtacct cgagtgattc gcggtgaatt tcgaggtatc
      121 tgcgcgccat tcgaggtatt gccgaaaaac agttgtccac gacgatggaa gcgacatgat
      181 tcggtactcc cgcgctttct tcgcagatga taagagaagc cccgcgcgaa actgcatcgt
      241 gaataaaatc atggccgttg acctgttcgc cgcgaaaagc gacaaaggcg tccccgggaa
      301 cgacaagtct gctgtcggca acgaaacgca ggggtatcgg accgtcgggg cctgtgagat
      361 gagtatcggc aagcgccgca atgcaagaaa gtcgaagatc gtttttcatc gccactctac
      421 ccctctttcg tcagcccatc ggcgaagagt atccccgtcc gaatatggaa tggattcaga
      481 gccgatacgc aagtattttt ccggcccttt cccggatact acaacgacat ctccgggttt
      541 tgccgaatcc atagcttcgt acactgcggc ggatctgtcg agaattgttt tcaagggaac
      601 ccttctttcg gaggattcga cgccttccac tatttgggcg gctatctgct ccgccggttc
      661 gttccgcggg ttgtccatcg tgacgatcac ttcatccgca agcgtagcgg cgatattccc
      721 caatgaaaac ctgttcggct ggaaacgttc tcccccgtgt ccgaatacag aaataatcct
      781 tcctccgcag agacgacgca cttccgtcag tacgttctgt agcgcgaaag gagtatgggc
      841 gaagtcgatg atacaactca ctccactatt gaaggaatac ctttccattc ttccgggaac
      901 ctgaatcatt gaatgtaagg aatcaacgag agccaatttg tccgtagcgt atggaagcgt
      961 tgcagagagc gcagctaaag cattgtacac gttgaatttt cccggaagag gcaggaaaac
     1021 aggaacgggt tcactgactc caggaagtga aagcgaaaaa cgtgttcctt caagagagct
     1081 ctgaagattg ctttcaagga gatgagcgcc atactctttc tctatcccga aagaaaccag
     1141 cttatcggaa taaaggccta aaagcc
//
LOCUS       DAOSWW010000983         1166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_115557_length_1166_cov_2.964896, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000983 DAOSWW010000000
VERSION     DAOSWW010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115557_length_1166_cov_2.964896"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PK442_14785"
     CDS             <1..226
                     /locus_tag="PK442_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88970.1"
                     /translation="RAFVHVSGESDYIGLSRVLSNEEKSASLLADAARDIDRFTTKYR
                     ALASYSKSLDSLLIVMDETKNEIALVGKGA"
     gene            223..759
                     /locus_tag="PK442_14790"
     CDS             223..759
                     /locus_tag="PK442_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88971.1"
                     /translation="MKTLKVAAKELNIDSSTLRKFAIKHGIPMERIRDATTGNQQACA
                     FNSDSFKKLQEARENLGFSAENKIQSIPETSGVFYFIHLIPEFDRRRVKLGFTSDVRG
                     RFQSHRCSAPTMEIADTWACKREWESAAIAAITNIDGVKQLGAEVFEFPDVDIALERA
                     NMFFHFFEQDISALPEDE"
     gene            949..1143
                     /locus_tag="PK442_14795"
     CDS             949..1143
                     /locus_tag="PK442_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88972.1"
                     /translation="MNNFLDPEEFCSIHKIDRRNLRRWAREGTYIEQIQLGPRTFRYR
                     LRNAGVEGERIGTVSDADAS"
BASE COUNT          305 a          285 c          312 g          264 t
ORIGIN      
        1 aagagcgttt gttcatgtca gcggagaatc cgactatatc gggctttcca gagtgctatc
       61 caacgaagag aaatccgctt cactccttgc cgatgcagcg cgggacattg atcgtttcac
      121 aacgaaatac agggcgcttg catcctactc aaaaagcctt gattccttgc tcattgttat
      181 ggacgagacg aagaatgaga ttgcgttggt aggcaagggg gcatgaaaac gcttaaagtt
      241 gccgcaaagg agttgaacat agactccagt acgctgcgga agttcgcaat caagcacggt
      301 atcccaatgg agagaatccg cgacgccaca acagggaatc aacaggcttg tgcgtttaat
      361 tccgactcgt tcaaaaaact tcaagaggcg cgggaaaatc tgggattttc cgctgaaaac
      421 aaaatccaat ccataccgga aacatcgggt gttttttact tcatccatct gataccggag
      481 ttcgaccgtc gccgcgtaaa actgggtttt acatcggacg ttcgcgggcg cttccagtct
      541 cacagatgtt cggctccaac gatggaaata gcggatacgt gggcatgtaa gagggaatgg
      601 gaatccgcag ctattgccgc gataacgaat atcgacggag taaaacagct cggtgcggaa
      661 gtcttcgagt tccctgacgt ggacattgcg ctcgaaaggg caaatatgtt tttccatttc
      721 ttcgagcagg acatttcagc actgccggaa gacgagtaat accccgccgc ctcccggcgg
      781 cgtctttatc actctttccc gcgcacctct tgctgagaac gggagttcac aagcgcgggc
      841 attccatacg ctgcggcttc tccggtgcag gtcaaaattt aaatacaacg ctatatgcac
      901 aatgacatgc gattgggccg gatggagccg tggcaaagga ggctgtacat gaataacttt
      961 cttgatccgg aagagttttg ttccatccat aaaatcgaca gacgaaatct gcgccgttgg
     1021 gcgagggagg ggacgtacat tgaacagatt caactcgggc cgaggacttt ccgttacaga
     1081 ctacgaaatg ctggcgtcga aggggagcgt atcgggaccg tatcggacgc ggacgcttcc
     1141 tagtctcgca tgggctttca acgaac
//
LOCUS       DAOSWW010000984         1166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_115561_length_1166_cov_2.843384, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000984 DAOSWW010000000
VERSION     DAOSWW010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115561_length_1166_cov_2.843384"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PK442_14800"
     CDS             <1..763
                     /locus_tag="PK442_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO88973.1"
                     /translation="LAEDNAVNTKMTVAMLKKTGLTVRHAQNGREAVEMWRSEPFDLI
                     LMDCEMPVMDGLEATRKIRSEEQDGKSIPIVALTAYAMKGDRERCLEAGMTEYISKPI
                     RSRKLYSLLARFLGTPSPSLHTDSEETPARFSAENGERERWESGFAELLLSLDGDREA
                     LAEMVEAFFSEVPELRGSLRSSLEKGDSSAAARALHKLKGVLGYLMGGEDISLFRVLE
                     IDARTEGLKTDDPRLRELHDLLDRYELFLREAASR"
     gene            892..>1166
                     /locus_tag="PK442_14805"
     CDS             892..>1166
                     /locus_tag="PK442_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HOO88974.1"
                     /translation="MNPQFARRMTRFQESDLTPILRLAANPEVISFAGGLPAPELFPV
                     EDLKKVAVKVLDAIGKRALQYSATEGHPPLREKIATRTNRNLKTSLTA"
BASE COUNT          249 a          321 c          350 g          246 t
ORIGIN      
        1 tcttgccgag gataacgcgg tcaatacgaa gatgaccgtc gcgatgctca aaaaaacagg
       61 gctcacggta cggcacgctc aaaacggccg cgaggcggtc gagatgtggc gcagcgaacc
      121 gttcgatctg attctcatgg attgcgagat gccggtgatg gatggattgg aggcgacgag
      181 gaagattcgc tccgaagaac aggacggaaa gagtattcct attgtagcgc tcaccgcgta
      241 tgcgatgaaa ggggaccggg agcgctgtct ggaggcgggc atgacggagt atatctccaa
      301 gcccattcgg tcccgcaagc tgtactcgtt gctcgccaga tttctcggga caccctctcc
      361 ctctctgcac accgacagcg aagaaacgcc cgcgcggttt tctgccgaaa atggagagcg
      421 ggagcgctgg gagagcgggt tcgccgaact gctcctctct ctcgacggag accgggaagc
      481 gttggcggag atggtggagg ccttcttttc cgaagtcccg gagctgcgcg gctcgcttcg
      541 gagctccctg gaaaaggggg attcctccgc agcagcgcgc gcgttgcata aactcaaggg
      601 agtacttggc tatctcatgg gaggagagga catctcatta ttccgcgttc ttgagattga
      661 tgccaggacg gagggtttga aaacggacga tccgcgcctt cgtgaactgc acgacctgct
      721 cgaccggtac gaactctttc ttcgtgaagc ggcttcccgc tgagtatccg ctttgtgcct
      781 gttcatgggg agttttctca ggtccctcac tttcgattga aaaggccatc ttttcggacg
      841 aaactcttcg gggtataatc ctctccaatc tttcgtccag gaggcattgc tatgaatcca
      901 cagtttgcac gaagaatgac gcgctttcaa gagtccgacc tgacgcccat cctgcgtctt
      961 gcagcaaatc ctgaagtgat ctcgttcgcc ggaggtcttc cggcgccgga gctcttcccg
     1021 gtcgaggatt tgaagaaggt ggccgtgaag gtgctcgacg ccatcggtaa aagggcgctt
     1081 cagtactccg ccacggaagg gcatcctcct ctccgcgaga agatcgccac gcgcaccaac
     1141 agaaacctga agacgtcgct gacggc
//
LOCUS       DAOSWW010000985         1165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_115698_length_1165_cov_3.779279, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000985 DAOSWW010000000
VERSION     DAOSWW010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115698_length_1165_cov_3.779279"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PK442_14810"
     CDS             complement(<1..689)
                     /locus_tag="PK442_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO88975.1"
                     /translation="MTEQQATVQKGNGGRSGKRWFLPFLTIFAVLAYLSFRASFSLIG
                     PLAWSALISFTVYPLYIRMRRRFFSRRSANLPALLATAVIVFAVLIPCLLGLFFAARE
                     GVRLLDSTEELLTSMEFSGEGAIATLLPEHIVSKIRPWMERYPTLRAYVRQTVARSAE
                     SAVDLVGSAILFLYYFSIITVANFFFLRDGHLIIGFLREIVPLPANESAAFFERTRQA
                     LQAVVYGVLVTA"
     gene            793..>1165
                     /locus_tag="PK442_14815"
     CDS             793..>1165
                     /locus_tag="PK442_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOO88976.1"
                     /translation="MILRGCASLRLFSTLFLTLLLWSGGAYAAGFPPAMIRDASRAVK
                     MLVTEGNGDRMGQGTGFFVTPDGLMLTNYHVVEGNRTIKIWDEKNLYDIVRIVAFDKN
                     ADVALLETNYPKSSVRHLPLRK"
BASE COUNT          244 a          345 c          375 g          201 t
ORIGIN      
        1 gccgtgacca gaacgccgta gaccacggcc tgaagcgcct gcctggtacg ctcgaagaag
       61 gccgcgctct cgtttgcggg aagcgggacg atctcccgca gaaaaccgat gatcagatgc
      121 ccgtcccgga gaaagaagaa gttggccacg gtgatgatcg agaagtagta gagaaaaaga
      181 atcgcgctgc ccacaagatc gacggcgctc tcggcgcttc gcgcgaccgt ctggcgcaca
      241 tacgcccgaa gcgtcgggta ccgctccatc cacggccgga tcttcgacac gatgtgctcc
      301 ggcagcagcg tggcgatggc cccctcgccc gagaactcca tcgaggtcag cagctcctcc
      361 gtgctgtcga gcaggcgcac cccctcgcgc gcggcgaaga aaagccccag cagacagggg
      421 atcaggacgg cgaagacgat gaccgccgtg gccagaaggg cgggcaggtt cgcggaacgg
      481 cgggagaaaa agcgccgccg cattcgtatg tacagcggat agactgtgaa ggagatgagc
      541 gcggaccagg caagaggtcc tatcagggag aaggaagccc ggaaggagag atacgcgagc
      601 accgcgaaaa tcgtcagaaa gggaagaaac catcgcttcc cgcttcgtcc gccgtttcct
      661 ttttgaactg tcgcctgttg ttctgtcatc tacgccaccc gctttcgtcc gattgtggta
      721 ttctcttttg agtaaggata ccactcggcg cggcgaatcg cctccgccgt tcgacgattc
      781 ggggtgatgt gtatgatttt acgaggctgc gcctcgttgc ggcttttttc gacgctcttt
      841 ctcacgctcc ttctgtggag cgggggcgcg tacgcggcgg gattcccgcc cgcgatgatc
      901 cgcgacgcct cccgcgccgt caagatgctg gtgacggagg gcaacggcga ccgcatgggg
      961 caggggacgg gctttttcgt cacgcccgac ggtctgatgc tgacgaacta tcacgtggtc
     1021 gaggggaaca ggacgatcaa gatatgggac gagaagaacc tctacgacat cgtccggatc
     1081 gtggcgttcg acaagaacgc cgacgtcgcc ctgctggaga cgaactatcc gaagtcgtcg
     1141 gtgcgccacc tcccgctgag gaagg
//
LOCUS       DAOSWW010000986         1164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_115885_length_1164_cov_3.569883, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000986 DAOSWW010000000
VERSION     DAOSWW010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115885_length_1164_cov_3.569883"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK442_14820"
     CDS             complement(<1..312)
                     /locus_tag="PK442_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HOO88977.1"
                     /translation="MDSGEFLAMLKDCIGSKVSLVIDAGLYKGTYPSRVEDVDEEAIG
                     LAHPMMRGALLPVHRNVQLKMSVEAGGALYFSVVSITKGLVRDSLPLLWVVPVGEARK
                     IQ"
     gene            complement(315..>1164)
                     /locus_tag="PK442_14825"
     CDS             complement(315..>1164)
                     /locus_tag="PK442_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOO88978.1"
                     /translation="RRKNIKGLPGLRSIAILSGKGGVGKSNVAVNLAMALAASGKRVV
                     LLDADLGMANVDLLCGISPKYTLAHLVKGQRSVDEVLVYLENGVMLLPGGPGVQELAE
                     LDDVAQSILIDKLSVLEGIADYLLIDTGAGIHKNTLSFGVAADMTILITTPEPTSIRD
                     AYGVLKTLVINTGSKIDVTLVVNMAASRSEGREVAERIQMAANQFLSLSVPYGGAMLL
                     DENVGKAVRMRTPFFLANPTCQASKDIAEIAANLASIDDETEPPTGRGVKSFFFRLAR
                     SLGLGV"
BASE COUNT          244 a          370 c          299 g          251 t
ORIGIN      
        1 ttgaatcttg cgggcttcgc cgacaggcac tacccagaga agcggcagag agtcccgaac
       61 cagtcccttt gtgatggaga ccacagaaaa atacagggct cctcccgcct ccacgctcat
      121 tttcagctgc acgttgcgat gcacgggcaa cagcgcgccc cgcatcatcg gatgggcaag
      181 gcctattgcc tcctcgtcga cgtcctctac tcttgaaggg tacgtcccct tgtagagtcc
      241 cgcgtcgatg acaagcgaca ccttgctccc gatgcagtcc ttgagcattg cgagaaattc
      301 gccggagtcc atttttacac tcccaacccg agacttctgg caaggcggaa gaagaaggat
      361 tttacacctc ttccggtcgg aggctcagtc tcgtcgtcta tgctcgccag attcgccgcg
      421 atctccgcga tatccttgct ggcctggcag gtcggattcg ccaggaaaaa tggagtacgc
      481 atccggaccg cctttccgac attctcatcc agcaacatcg ccccgccgta aggaaccgag
      541 aggctgagaa actggttcgc cgccatctgt atgcgctcgg cgacctctct tccctcggaa
      601 cgcgaggccg ccatattcac gacgagcgtc acgtcgattt tgctgccggt attgatgacc
      661 agcgtcttca gcacaccgta cgcgtcgcgt atggatgtcg gctcaggggt tgtgatcaag
      721 atcgtcatat ccgcagcaac gccgaaggag agcgtattct tatggattcc ggcccctgta
      781 tcgataagca agtaatctgc tattccctcc agaaccgaga gtttgtcgat tagaatcgat
      841 tgggcgacat catcgagttc cgcaagctcc tgcactcccg gtccccccgg gagaagcatg
      901 accccgtttt ccagatagac aagcacctcg tccacgcttc gctgcccctt caccaggtgc
      961 gcaagagtgt acttcggcga aattccgcag agaagatcga cattcgccat cccgagatcc
     1021 gcatcaagca gaacgactcg cttccctgaa gccgcgagcg ccatcgccag attcaccgcg
     1081 acgttgctct ttcctactcc gccttttccg ctgagaatgg cgatggaacg gaggcccggc
     1141 agccccttga tgttcttacg gcgt
//
LOCUS       DAOSWW010000987         1162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_116228_length_1162_cov_3.679313, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000987 DAOSWW010000000
VERSION     DAOSWW010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116228_length_1162_cov_3.679313"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PK442_14830"
     CDS             <1..558
                     /locus_tag="PK442_14830"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HOO88979.1"
                     /translation="ATRNSPSGATSAAAAVIAGRAIDWSEFRDHLFPILTAFSIVGVI
                     TFKIFSKYRITKEKHQAKLMKTLYFHNLDNNAGVFDFLVQEAEEEECKEALLAYYFLL
                     TEKDANGEPFTEEGLDRRIEQWMEERFGMTLDFEVDDALRKLEEKKLLVKDGDRYRVP
                     PIDETLIRLDELWDGVFAYSNERKE"
     gene            872..>1162
                     /locus_tag="PK442_14835"
     CDS             872..>1162
                     /locus_tag="PK442_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO88980.1"
                     /translation="MIIVRISLLGVYRRRLCSLALFLSLFFVSASEGAVLFVDAGAAG
                     ANDGTSWTDAYTDLQTALTTAADDDEIWVAAGIYRPTGDADRTQSFILRNSIA"
BASE COUNT          278 a          313 c          327 g          244 t
ORIGIN      
        1 gccacgagaa actcgccatc tggtgcgacc agtgccgcag ccgcggtgat cgccgggcgc
       61 gcgatcgact ggagcgagtt ccgggatcac ctcttcccga ttcttaccgc gttctccatc
      121 gtcggcgtga tcaccttcaa gatcttctcc aagtacagga tcacgaagga gaagcaccag
      181 gcaaagctga tgaagacgct ctacttccac aacctcgaca acaacgcagg ggtctttgac
      241 tttctcgtgc aggaggccga ggaggaggaa tgcaaggagg cgctgcttgc ctactacttc
      301 ctgctgacgg agaaagacgc gaacggagag ccgttcacgg aagaagggtt ggaccgccgc
      361 atcgagcagt ggatggaaga acgcttcggg atgacgctgg acttcgaggt ggacgacgct
      421 ctccgcaagc tggaggagaa gaagctcctc gtaaaggacg gcgaccggta ccgagtcccg
      481 ccgatcgacg aaaccctgat ccgcctcgac gagctgtggg acggcgtttt tgcgtacagc
      541 aacgaaagaa aagagtagaa gaagcaagcc cggtagcttc atgatcgccg aaacagatta
      601 ttcttgcccc ctcgtccaaa ggtttctgct cacacacatc ctccggctca tctttacagg
      661 gtcgattgag cattattgcg aagactcttg ctattgctgc atctaaaaag aggggtggga
      721 aaaaccacct ctcttttttc tgcggatgca gttctacaat aaacgcactg gagcgctcga
      781 atgctccaat tgttgttgtg cacgctaaaa gcggccgctg ttcagcagga gtatcgctcc
      841 cgcagttcag cgaaacaaaa ggagatagga gatgatcata gtgcgtattt ctttactcgg
      901 agtctacagg cggcgtttgt gttctctggc gctttttctg tcgctctttt tcgtatcggc
      961 atcggaaggg gcggtccttt ttgtcgacgc gggagcagca ggagcaaacg acggcacatc
     1021 atggaccgac gcctataccg atcttcagac agcactgaca actgcggccg acgacgacga
     1081 aatctgggtg gcggcaggta tctacagacc gacaggcgac gcggatcgta ctcaatcttt
     1141 catcctgaga aattccatag cc
//
LOCUS       DAOSWW010000988         1158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_116915_length_1158_cov_3.310063, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000988 DAOSWW010000000
VERSION     DAOSWW010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116915_length_1158_cov_3.310063"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1158
                     /locus_tag="PK442_14840"
     CDS             <1..>1158
                     /locus_tag="PK442_14840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO88981.1"
                     /translation="VIEPDVPALCRGDPGRLRQILNNLTSNAVKFTERGEVTTTVSLV
                     GQDARFASLRFTVRDTGIGISPETMELLFEKFSQGDASISAKYGGTGLGLVISKQLVA
                     LMGGEIGVRSEEGKGSEFWCTVVLEKLSEAEFSAAGARGATEEPLSGFERRGRRILLA
                     EDNITNQRVALGMLKKIGVTADAAENGEAALEALASCSYDLVLMDCRMPVMDGYEATR
                     RIRRMEGDVRNIPVIALTAYAMPGDREKCLEAGMNDYLAKPIIRDALLAVLERWLPRR
                     EKEEGPFEDVATEGAREMEAQHLESVWDRDVLLERLLGDEEMLEEIITGFLEDAPAQL
                     SALSEALATGDAASAEMSAHAIKGAAAGIGAERVRCAAFAVENAVRNGAMDK"
BASE COUNT          246 a          299 c          398 g          215 t
ORIGIN      
        1 gtcatcgaac cggacgtccc ggcgctgtgc cggggggatc ccggccgttt gcgtcagata
       61 ctcaacaacc ttacgagcaa cgccgtcaag tttacggagc gcggcgaagt gacgacaacc
      121 gtctcgctgg tcggacagga tgcgcgtttc gcgtctctgc gttttaccgt tcgggatacg
      181 ggaataggaa tcagccccga aacaatggag ctgctctttg aaaagttcag ccagggagac
      241 gcgtcgatct ccgcaaagta cggcggcaca gggcttggtc ttgtaatttc gaaacagctt
      301 gtcgccttga tgggaggcga aataggggta cggagcgaag aggggaaagg atccgagttc
      361 tggtgtacgg tcgtcctgga gaagttgagc gaggctgagt tttctgcggc cggcgcacgg
      421 ggcgcgacag aagagcccct gtccggtttt gaacggcggg gacgacgaat cttgctggcc
      481 gaggataata tcactaacca gagggttgcc ctgggcatgc tgaagaagat cggcgtgacg
      541 gcggatgccg ccgaaaacgg cgaagctgcg ctcgaagccc ttgcctcatg ctcttacgac
      601 cttgtgttga tggattgccg gatgcccgtg atggatggat acgaggcaac ccgcagaatc
      661 cgccgcatgg agggcgacgt ccggaatatt cccgttattg cactgactgc gtacgccatg
      721 cccggagaca gggagaaatg ccttgaagcg ggcatgaacg actacctcgc caagccgatt
      781 attcgcgatg cgctgctcgc tgtgctggaa cgctggctcc cccgccggga gaaggaagaa
      841 gggccgtttg aggacgttgc gacggaagga gcccgggaaa tggaagcgca acatctggag
      901 tcggtctggg acagggacgt cctgttggaa cgtcttctcg gggatgaaga gatgctcgaa
      961 gagatcatca caggctttct ggaggacgcg ccggcgcagc tttctgcgct ttccgaagct
     1021 cttgcgacgg gagacgccgc ttccgcggag atgtccgcgc acgcaatcaa gggagctgcg
     1081 gcgggtatcg gagcggagcg ggtccgatgc gcggcgttcg cggtagagaa tgccgtacgc
     1141 aacggagcga tggacaaa
//
LOCUS       DAOSWW010000989         1157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_117087_length_1157_cov_3.367514, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000989 DAOSWW010000000
VERSION     DAOSWW010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117087_length_1157_cov_3.367514"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PK442_14845"
     CDS             complement(<1..613)
                     /locus_tag="PK442_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO88982.1"
                     /translation="MSHDYDVLVVGGGVVGCAIARELSRFKVSAAVLEKEMDVGLGTS
                     CRNSGVLHAGFNYAPGSLRAVLDVKGNSMMDDLCRDLKVKIKRIGKLTVALDEEDEGT
                     LQRLLEQGRANGVPGLEIIGKERMQAIQPGVEGYMALYSPTSSIISPYALTIGLAENA
                     LANGVNFHLGQQVVSVTPLAGKGWEVGTKSGERFACAVLVNASG"
     gene            801..>1157
                     /locus_tag="PK442_14850"
     CDS             801..>1157
                     /locus_tag="PK442_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO88983.1"
                     /translation="MNKTRKELIHRLSDLITEGDIVSEGKLPPERELAAILGTTRPLL
                     REALIALEALGVLDIRDRQGIFLAEEKVGEIGRVLGQAQVWPLELLSQVMEIRQLLDP
                     GAAALAALRRKERDIAK"
BASE COUNT          250 a          362 c          309 g          236 t
ORIGIN      
        1 gtccggaagc gttcaccaaa accgcgcagg cgaaacgttc tcccgacttc gtcccgacct
       61 cccacccctt tccggcgagc ggcgtgaccg agacgacctg ctgccccagg tgaaagttca
      121 ccccgttggc cagcgcgttt tccgcaaggc cgatcgtaag cgcgtagggc gagatgatcg
      181 agctggtggg cgagtagagc gccatatacc cctccacacc cggctgaatc gcctgcatcc
      241 gctccttgcc gataatctcc agaccgggaa cgccgtttgc ccgcccctgt tcgagaagac
      301 gctgaagcgt tccttcgtcc tcttcgtcga gggcaaccgt cagcttgccg atacgcttga
      361 tcttcacctt gagatcgcgg cagagatcgt ccatcatcga gtttcccttc acgtcgagaa
      421 ccgcacgcag cgatcccggc gcatagttga atcccgcgtg gagcacgccg ctgttccggc
      481 agctcgtacc gagtccgacg tccatctcct tctccaggac tgcggcgctc accttgaagc
      541 gcgacagctc ccgggcgatg gcgcacccta ctactccacc gccgacgaca agtacatcgt
      601 agtcatgcga caacctgcaa cacctgcttt caaagaaaaa gacgtttttt tcgcatactg
      661 gtcgaccaag gtattccaat tatccggcga ctatagtatc atagcctcca aagcaagaca
      721 agataaatgc actgaaaatt tcaagttctt tttttaacaa atcgatagtg tcatgtcgat
      781 atctgcttgg ggaggcctcc atgaacaaga ctcgaaaaga actgatccac aggttatcag
      841 atttgatcac ggagggggat atcgtttcgg agggcaagct cccccccgaa agggagcttg
      901 ccgccattct ggggacgaca cggccgctcc tccgggaggc gctcatcgct cttgaagcgc
      961 tgggcgttct ggatattcgt gatcgacagg gtattttcct cgccgaagag aaggtaggcg
     1021 aaatcggacg cgtcctggga caggcacagg tgtggcccct cgaacttctc tctcaggtga
     1081 tggagatccg ccagctcctc gatccgggag cggccgcact cgccgcgctt cggcgaaagg
     1141 aacgcgacat cgccaag
//
LOCUS       DAOSWW010000990         1157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_117147_length_1157_cov_2.211434, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000990 DAOSWW010000000
VERSION     DAOSWW010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117147_length_1157_cov_2.211434"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PK442_14855"
     CDS             complement(<1..477)
                     /locus_tag="PK442_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO88984.1"
                     /translation="MALQQIKKVIVRDAIIENVLQYIEENRLEIGDKIPSERTLASCL
                     KVSRGSVREALKALEYNGILEIHHGGGAFLRSKSPFPLTRYGGDERKNLVMLRHLLDA
                     RRMIEERVIVEATPAATAQQIRELYEMEERQVTLLEKGLVEAGSKYELPNMNFELSI"
     gene            842..>1157
                     /locus_tag="PK442_14860"
     CDS             842..>1157
                     /locus_tag="PK442_14860"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOO88985.1"
                     /translation="MERERHVREMTETIGKFIKYFGKILPDDVQKKLKELAEAEVSPM
                     ATLTYETMLRNQELARQLDRPSCQDTGVLQFFARVGTKFPLIDDLPEIFRNAVLDATE
                     RAP"
BASE COUNT          271 a          306 c          301 g          279 t
ORIGIN      
        1 gatgctcagt tcgaagttca tgttcggcag ttcgtacttc gatccagcct ccaccaaccc
       61 cttctcgagg agggtgacct gacgctcctc catttcatag agttcgcgaa tctgctgcgc
      121 tgtcgccgca ggcgtcgctt ccacgatgac gcgctcttcg atcatccgtc tggcgtcgag
      181 gagatgtcgc agcatgacca ggttcttgcg ctcgtctccg ccgtatctcg tcagcggaaa
      241 gggggatttt gagcggagaa aggctccgcc tccgtgatga atctccaaaa tgccgttgta
      301 ttcaagagcc ttgagcgctt cccgcactga ccctctgctg accttcaggc aggaggcgag
      361 cgtgcgctcc gaaggaattt tatcccctat ctccaacctg ttctcttcaa tgtattgaag
      421 gacgttctcg ataatcgcat cccggacgat gacttttttg atctgctgta gggccatgcg
      481 tgttcattct cccctttact tttaaaacgc aatctgttac aatcttcttt acaagctggt
      541 cctaccagtt accaatttga accatgcgta acactcgttc cactccgttg ttttcaaaca
      601 acgcacatgc gctgaatggg ggtgcactgc aaaagcatat catgttttga aacttttttt
      661 cggacaaaag ggcttcgcgc cgcgaaattc gccgagaacg ccgtgtacga gaaatcgatc
      721 tgcttctctt tggacacctc ggatacccgc agaaataagg atgtctcgga tcagcacccc
      781 gaaaagggtg cgcacccggt agcagcctgt agatgaacca gatcttaaag ggggggaatt
      841 gatggaaagg gaacgccatg tccgcgaaat gacggaaacc atcggtaaat tcattaagta
      901 cttcggcaag atactgcccg atgacgtgca aaaaaagctc aaagagcttg cggaagccga
      961 agtcagcccg atggcgacgc tgacgtacga gacgatgctg agaaaccagg agctggcaag
     1021 gcagttggac agaccaagct gtcaggatac gggagtcttg cagttttttg cgcgggtggg
     1081 aacaaaattt ccgctgattg acgatctacc ggagattttc cgcaatgcgg ttctggatgc
     1141 gacggagcgc gctccgt
//
LOCUS       DAOSWW010000991         1155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_117404_length_1155_cov_3.370909, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000991 DAOSWW010000000
VERSION     DAOSWW010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117404_length_1155_cov_3.370909"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PK442_14865"
     CDS             complement(<1..734)
                     /locus_tag="PK442_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOO88986.1"
                     /translation="MARETRNTRKKRVGESTKKGGKLKYIPLGGLGQIGKNLHVFEYE
                     DSILVVDCGLMFPDDEMLGIDFVIPDISYLVENKEKIVGIVVTHGHEDHIGGLPFALK
                     KISAPLYGTKLTLGMVGNKLAEWAPELNPDYREIQAGDVVEMGPFKVRFIAVCHSIPD
                     GVGLAIGTPVGTVVHTGDFKLDPTPIDGRLTEYDAFSEEGKKGVLLLLSDSTNVERPG
                     FTESERVLSGTLDKIFRQYRSKRIVIA"
     gene            920..>1155
                     /locus_tag="PK442_14870"
     CDS             920..>1155
                     /locus_tag="PK442_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOO88987.1"
                     /translation="MTRKGYEKLMAELVELRSTGRAEISRQLEEARSFGDLSENAEYA
                     AAKEEQAKLETRISRLEFQLSNAKILDSSSIDAS"
BASE COUNT          293 a          349 c          264 g          249 t
ORIGIN      
        1 gcgatgacga tacgcttcga ccggtactgt ctgaagatct tgtcgagcgt tccggagagc
       61 acccgttccg actcggtgaa tccgggtctt tccacatttg tcgaatctga aagaaggagc
      121 agcaccccct tcttcccctc ctccgaaaac gcgtcgtact cggtaaggcg cccgtctatc
      181 ggcgtaggat caagcttgaa atcccccgta tgcaccactg tgccgacggg cgttcctatg
      241 gccaggccaa caccgtcggg aatggagtga cagaccgcga taaaacggac cttgaacggc
      301 cccatctcca ccacgtcgcc ggcctgaatc tcccggtagt ccggattcag ttcgggcgcc
      361 cactccgcaa gcttattgcc caccatacca agcgtcagct tcgtcccgta gaggggagcc
      421 gagatcttct tgagggcgaa cggaagccct ccgatgtggt cctcgtgccc gtgcgtcacc
      481 acgataccga cgattttttc cttgttctca acgaggtagg aaatatccgg gatgacgaaa
      541 tcaataccga gcatctcgtc gtcggggaac atcaatccgc agtccaccac aagaatcgaa
      601 tcttcatatt caaacacatg aagattcttt cctatctgac caagccctcc cagagggata
      661 tacttcagct ttcccccttt tttcgttgac tccccgactc tcttttttct cgtatttcgc
      721 gtttcgcgtg ccataaatcc accttgcctt tcaaaatttc ggctatcttc gctacaaaaa
      781 atgcaaagac gtattgccga agagaaattt ctttgttaga attccatatc aaccgcaata
      841 cacccaatcc tcaaaatgag gatatattcg ggaggttaca agtttgaaca acaccaacac
      901 caacgacagc ccatccacaa tgacccgaaa agggtacgag aagctcatgg ctgagctggt
      961 cgaactccgc agcacaggaa gagcggagat ttcacggcag ctcgaagagg cccgatcatt
     1021 cggcgatctc agtgaaaacg ccgagtacgc cgcggcgaaa gaagaacagg ccaagctgga
     1081 aacgcgcatc tctcgactcg aattccagct cagcaacgca aaaatcctcg actcctccag
     1141 catcgacgcc agcag
//
LOCUS       DAOSWW010000992         1154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_117611_length_1154_cov_2.631483, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000992 DAOSWW010000000
VERSION     DAOSWW010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117611_length_1154_cov_2.631483"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PK442_14875"
     CDS             complement(<1..919)
                     /locus_tag="PK442_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO88988.1"
                     /translation="MFPRKKDISQCLRLVEEDKAITSPAAKLPYYPLVVRRGRGAVVE
                     DVDGNSYIDMLSSAGAVNTGHCHPKVVEAIKKQTEELILYTHVYMYHEPLIDLTKELL
                     SITPGGYRKRAYYGLSGSDANDGIIKLARIATKRPKILAYLRAYHGSTYGALSMSAVS
                     LPMHRGLGPLLPDIYHVPYPDVYRTPFPGMTEDEVADYAIEQIRIAFSTNIPPDETAA
                     IVIEPIQGDSGLVVPPKKYMQSLRKLCDEYGILLVSEEVQQGFGRTGKWFGIENFDVV
                     PDAIVMGKAIASGLPLGAVVARADIMETWE"
BASE COUNT          183 a          348 c          376 g          247 t
ORIGIN      
        1 cctcccacgt ctccatgatg tcggccctgg cgacgaccgc gccgaggggc aggccggaag
       61 cgatggcctt tcccatcacg atcgcgtcgg gaacgacgtc gaagttctct atgccgaacc
      121 atttgccggt gcggccgaac ccctgctgta cctcctcgga gacgagcagg atgccgtact
      181 cgtcgcagag cttccgcagg gattgcatgt actttttcgg cggcacgacg agcccggagt
      241 cgccctggat cggctcgatg acgatggcgg cggtctcgtc gggggggatg ttcgtcgaga
      301 aggcgatgcg gatctgttcg atggcgtagt ccgcgacctc gtcctccgtc atccccggga
      361 agggagtgcg gtagacgtcg gggtacggca cgtggtagat gtcggggagc agcggtccca
      421 gcccccggtg catcggcagg ctgaccgcgc tcatcgagag cgcgccgtag gtgctcccgt
      481 ggtaggcgcg gaggtacgcc aggattttcg gacgcttcgt cgcgatgcgc gccagcttga
      541 tgatgccgtc gttcgcgtcg gagcccgaga ggccgtagta cgcccgcttc cggtagccgc
      601 cgggagtgat cgagagaagc tccttcgtca ggtcgatcag cggttcgtgg tacatgtaga
      661 cgtgcgtata gaggatgagc tcctccgtct gcttcttgat cgcttcgacc accttcgggt
      721 ggcagtgccc ggtgttgacg gccccggcgc ttgaaagcat gtcgatgtag gagttgccgt
      781 cgacgtcctc cacgactgcg cctctccccc tgcggacgac cagaggatag taggggagtt
      841 tcgcagccgg ggaggtgatg gccttgtcct cctccacgag acggagacat tgactgatat
      901 cctttttccg ggggaacatt cctgcggtca tctcctttaa aaaagggcgg aaggcatgcg
      961 cgccctccgc ccctttgcat gtttcggaat cagcgggcta ttccggattc cttgtagatt
     1021 ctgtccataa gctccttgcc cacgagttcc tccatcttcg gccacaccgt cttgcggacg
     1081 tgggtcgcga gggcgtcgag ctcctcctgg gagagcttca cgatggtcca gccgtactct
     1141 ttggccaatt tctg
//
LOCUS       DAOSWW010000993         1153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_117748_length_1153_cov_3.081967, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000993 DAOSWW010000000
VERSION     DAOSWW010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117748_length_1153_cov_3.081967"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PK442_14880"
     CDS             <1..488
                     /locus_tag="PK442_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOO88989.1"
                     /translation="EEDRSRLLRRLEEVFQPDRYRARVFGVSQLGLVEITRKRARPDI
                     RSMLTRSCPACGGGGWVFREDTIAMAIKRFLRKVSFANRAEALLLEANAAVAQYIFET
                     YLMLWEAELGRKIFIAAAPDFAWSKFRLEAQGGVDQVERRIEQMEKREARIIVYRTSS
                     S"
     gene            463..>1153
                     /locus_tag="PK442_14885"
     CDS             463..>1153
                     /locus_tag="PK442_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO88990.1"
                     /translation="MYIERLHLKGFKSFGASLDLLLSPGFTAIVGPNGSGKSNLLDAL
                     RWVLGEAGSQRLRIVKQGDLLFSGSVTLPPASRAEVSLLLRSTPHSGRESAHSPEAGQ
                     CVLRRSYSSETGTVLTVDGTRVRLSDLDEVKRLWHLEGDQFAFIGQGEVAEAIHQRPA
                     QRRAHLEVLFGIDQYRKKRNETSQKLVAASEEVLRLGALAAELTHRREEIAPAVEIAQ
                     AAKKITDELEEK"
BASE COUNT          226 a          352 c          365 g          210 t
ORIGIN      
        1 ttgaagagga ccgttcccgc ctgctcagaa ggctggagga ggtcttccag cccgacagat
       61 accgcgcgcg cgttttcggc gtctcgcagc tcggacttgt agagatcacg cgcaaacgag
      121 ctcgtcccga catccgctcg atgctcaccc ggagctgccc cgcgtgcggc ggaggcggat
      181 gggtcttccg cgaggatacc atcgcgatgg ctattaagcg gttcctcagg aaggtctcct
      241 tcgccaacag ggcggaggcg ctgcttcttg aagccaacgc cgcggttgcc cagtatatct
      301 tcgagaccta cctcatgctc tgggaggcgg aactcggacg gaagatcttc atcgccgccg
      361 ccccggactt cgcgtggagc aagttccgcc ttgaggcgca agggggcgtc gaccaggtgg
      421 agcgtcgtat agaacagatg gagaagcggg aggcacgaat cattgtatat cgaacgtctt
      481 catcttaaag gcttcaagag cttcggagcc tctctggatc tcctcctctc accaggattc
      541 acggcaatcg tcggtcccaa cggaagcggc aagagcaacc tgctcgacgc gcttcgttgg
      601 gtgctgggcg aagccggttc gcaacgcctc cgcatcgtca aacaggggga tcttctcttc
      661 tccgggagcg tgacgctgcc tccggcttcc cgcgccgagg tctctcttct gttgcgctcg
      721 acgccgcact caggccggga gagtgcgcac tctccggagg ccggtcagtg cgtgctgcga
      781 cggagctatt cttccgaaac agggacggtg ctcaccgtcg acggaacgcg cgtccggctt
      841 tcggatctcg acgaggtgaa gcgtctctgg catctcgaag gagatcagtt tgccttcatc
      901 gggcagggag aggtcgccga ggcgatccat caacgccccg cgcagcgacg cgcgcacctt
      961 gaggtgctct tcggcatcga ccagtaccgg aaaaagagaa acgaaacgtc gcagaagctc
     1021 gtcgccgcat ccgaggaggt tctgcgtctc ggcgcgctcg ccgcggagtt gacgcaccgc
     1081 agggaggaga ttgctccggc cgtagaaatc gcgcaagccg cgaagaagat caccgacgaa
     1141 ctggaagaaa aga
//
LOCUS       DAOSWW010000994         1149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_118443_length_1149_cov_3.242230, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000994 DAOSWW010000000
VERSION     DAOSWW010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118443_length_1149_cov_3.242230"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PK442_14890"
     CDS             <1..1101
                     /locus_tag="PK442_14890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO88991.1"
                     /translation="PEEAKPPRDTGQKVALRDPVTGLSTRAFFFEELQQMDSERYLPL
                     SVIRGDLNGMKLINSVFGHIAGDKLLKAGARVLRKHSRNSDILVRWEGDEFLLLLPRT
                     KEDDAATIVERIEDAFKKTKIREIPVPPSISMGYSAKVHRWQDFGNVLKDAEEDMREK
                     QLSESRKVRTKLLSSLQKSLDEHTPETEEHISSMRRIARIMSDLLQLSPEERELLDLA
                     VRFHDIGKASIPVYLLNKIAPITDEDRETLRRHAEIGGKIAEAATSETAKSAPVIRAH
                     HERWDGTGYPSGIAGEDIPLLSRIISIADAFDVMTRGTAYKDAVSPEEALEELERLAG
                     LQFDPRLVDLFAEHAEKFLTDESPETLPERRK"
BASE COUNT          278 a          328 c          337 g          206 t
ORIGIN      
        1 cccgaagaag caaagccccc ccgcgacacc ggacagaaag tcgcgctacg cgatccggta
       61 acgggtcttt ccacacgcgc ctttttcttc gaggaactac aacagatgga ctctgaacgc
      121 tacctgccgc tgagcgtcat ccgcggagat ctgaacggca tgaagctgat caacagcgta
      181 ttcgggcata ttgcggggga taagctcctc aaggccgggg ccagggtcct ccggaaacat
      241 tcgagaaaca gcgatatcct cgtccgctgg gagggcgacg aatttttgct tctgctcccg
      301 aggacgaagg aagatgacgc tgcaacaatc gtcgagcgca tagaggacgc gttcaaaaag
      361 acaaaaatca gagagatccc ggttcctccg agtatttcca tggggtactc cgccaaagtg
      421 caccgatggc aggacttcgg caacgtcctc aaagacgccg aggaggacat gcgcgagaag
      481 cagctgtcgg agagcaggaa ggtccggacg aagctgctct cctcgctcca gaagtcgctc
      541 gacgagcata cgccggaaac cgaggagcat atcagttcca tgcgccggat tgcccgaatc
      601 atgagcgacc ttctgcagct ctccccggaa gagcgggagt tgcttgacct ggccgtccgt
      661 ttccacgaca tcggcaaagc ctcgattccc gtgtatctgc tgaataaaat cgccccgatc
      721 accgacgaag accgcgaaac gctccgcagg catgcggaga tcgggggaaa aatcgcggag
      781 gcggcgacgt ccgagacggc gaagtcggcg cctgttattc gagcgcacca tgaacgatgg
      841 gacggcacag gctacccctc ggggattgcc ggagaggata ttcccctgct ctcccggatc
      901 atttccattg ccgacgcgtt cgatgtgatg accagaggaa cggcctacaa ggacgccgtt
      961 tctcccgagg aagccctgga agagctggag aggctcgccg ggctgcaatt cgacccgcgg
     1021 cttgtcgatc tcttcgcaga acatgcggaa aagtttttga cggatgaatc tccggaaact
     1081 cttcccgaac gcagaaaata gggtagaata ggtctgaagc cgttgtttgc ggcatcttaa
     1141 tcagcaagg
//
LOCUS       DAOSWW010000995         1148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_118652_length_1148_cov_2.487649, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000995 DAOSWW010000000
VERSION     DAOSWW010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118652_length_1148_cov_2.487649"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PK442_14895"
     CDS             <1..379
                     /locus_tag="PK442_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOO88992.1"
                     /translation="FLFRLLLKAAPLLLFFLFSKILKDMLRGAQGSPGPSGGARGRSS
                     SGGGAAYTPPPGGKRDPYTVLGCSPSSSNEEIKKRYRELLAKYHPDKFIGQNLDEDFV
                     ALASRKFQEIQEAYGRIRSARGI"
     gene            574..>1148
                     /locus_tag="PK442_14900"
     CDS             574..>1148
                     /locus_tag="PK442_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOO88993.1"
                     /translation="MSTLELFTGPESFSRIKSQARTTIESAFFGNNVVPVSNLKEAYE
                     LARVSPGTVELTGMPVYRPEGQGLPPRSNVLLMNDGAVVGRCAAARRIVGEPGVNMGD
                     YAAVLREAVYNSRFRTFYSAEAYIGLEGDFMVKAHLLAPKNHENLVYNWLLNFQYLNR
                     TYLDMYAASRPLPDGDIFMFADPDWTSPDYP"
BASE COUNT          212 a          348 c          336 g          252 t
ORIGIN      
        1 gtttctgttt cgacttcttc tgaaggcggc cccgttgctg ctcttctttc tcttttccaa
       61 aatactgaag gatatgctcc gcggggcgca aggctcgccg gggccgtccg ggggcgcgag
      121 gggcaggtct tcctccggag gaggcgccgc gtatacgccc cctcccggcg ggaagcgcga
      181 cccctatacc gtgctcggct gttcgccttc ctcctccaac gaggagatca agaagcgcta
      241 ccgggagctg ctcgcgaagt accaccccga caagttcatc gggcagaatc tcgacgagga
      301 ctttgttgcc ctcgcctcaa ggaagtttca ggagattcag gaggcgtacg gccggattcg
      361 ctccgcacgg gggatatagt ttttatcttt cttttcttcg gagcgcggaa cccgacggca
      421 tctttcttgc gcgactgcgg cgggttcttc ggagcgcggc gtgcgtggcg acggtttcac
      481 accgcggtat ggagtgtgat acaatgacgc cgccagagct cccctttcga ggggaaaaac
      541 gttcccgcat gcgggagaaa gaggcgaacc tccatgtcaa ctctggaact ttttaccgga
      601 cccgagagct tctctcgtat caagtcacag gcgcggacga ctattgaatc cgcttttttc
      661 ggcaacaacg tggttcctgt ctcgaacctc aaggaggcct acgagctggc gagagtctcc
      721 cccggcacgg tcgagcttac cgggatgccg gtctaccgtc ccgaagggca gggattgccc
      781 ccgcgcagca atgtgctcct gatgaacgac ggcgctgtgg tcggccgttg cgccgccgcg
      841 cggaggatcg tcggcgaacc cggcgtaaat atgggagatt atgcagcggt gctccgggaa
      901 gcggtataca actcccgttt tcgtaccttt tactccgccg aggcgtatat cggcctcgaa
      961 ggtgatttca tggtgaaggc gcatcttctc gcgccgaaga atcatgaaaa tctcgtctac
     1021 aactggctgc tgaacttcca gtatctcaac aggacctatc tcgacatgta cgcggcatcc
     1081 aggccgctgc cggacggcga tatcttcatg ttcgccgatc ccgactggac ctctcccgac
     1141 tacccctt
//
LOCUS       DAOSWW010000996         1146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_118957_length_1146_cov_3.234647, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000996 DAOSWW010000000
VERSION     DAOSWW010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118957_length_1146_cov_3.234647"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1146
                     /locus_tag="PK442_14905"
     CDS             <1..>1146
                     /locus_tag="PK442_14905"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOO88994.1"
                     /translation="DEVVFFPNHFTIRAGDLEGREHILSPGLAEKALSRGWSAHEEEG
                     LRGFDFAKAFQDPGSWKNDGNTYRHRHGVAAFRGPLLAEEADLPFAVLPERLITPEDV
                     KAVLRSHYESTPDDIRSDFAGRSPHFTSVRRICTGGTVESMVALLSDDPLFTELLISR
                     GRPCCDPYQPYWGGMTTLPKELSPIMEPRSAIDAHCAPEPGLLDISDAAWWQLFHEQT
                     LADLLYEHIHPALSEWVARREEELSAMAPKIAAEARVLAQSGETDAARRLLTEESAAL
                     AGASAVRKAEFLSAYGPRVLSPERAEIVKGDISGELTVFFQSQSAPVEDSFLMGQGGT
                     PPQRWAAPLRGSLHFADGAWSLRFRVRELTEAAVPCLADFWLAGRDE"
BASE COUNT          195 a          374 c          383 g          194 t
ORIGIN      
        1 cgacgaggtc gtcttcttcc ccaaccactt caccatccgg gcaggcgacc tcgaaggccg
       61 cgagcacatc ctgtcgccgg ggctggcgga gaaagccctc agtcgaggat ggagcgcgca
      121 cgaagaggag gggctccgcg gtttcgactt cgcgaaggcc ttccaggatc caggctcgtg
      181 gaaaaatgac ggcaatacct acaggcacag gcacggcgtc gcggcgttcc gcgggccgct
      241 gctcgcggag gaagccgacc tcccgttcgc tgtgcttcct gagagactga tcaccccgga
      301 agacgtcaag gccgtgctcc gcagtcacta cgaaagtacg ccggacgata tccggagcga
      361 tttcgcgggg cgatctccgc acttcacctc cgttcgccgc atctgtaccg gcggcacggt
      421 cgagagcatg gtggcgctgc tctcggacga cccgctcttc accgaactcc tgatctcgcg
      481 cggacgtccc tgctgcgatc cctatcagcc ctactgggga ggaatgacga cgctgccgaa
      541 ggagctttcc ccgatcatgg aaccccgaag tgcgatcgac gcgcactgcg ccccggagcc
      601 gggacttctc gacatctcgg acgccgcgtg gtggcagctc tttcatgagc agacgctcgc
      661 ggatctgctc tatgagcata tccacccagc cctttcggag tgggtcgcga gacgcgaaga
      721 ggagctctcg gcgatggctc cgaagatcgc cgcggaggcg agggtgttgg cccaaagcgg
      781 cgaaacggat gccgcccggc gtctgttgac ggaggaaagc gctgctttgg ccggggcgag
      841 cgccgtgcga aaagcggagt ttctttccgc gtacgggcct cgcgtactca gtccggagcg
      901 cgcggagatc gtcaaggggg atatctcagg tgagctgacc gtcttctttc aatcgcagtc
      961 tgccccggtc gaagactcct tcctgatggg gcaggggggg acgcccccgc agcggtgggc
     1021 cgctcccctg aggggaagcc tccacttcgc cgacggcgcg tggtcgctcc gcttccgcgt
     1081 gcgagagctc accgaggcgg cggtcccgtg cctggcggac ttctggctcg ccgggcgcga
     1141 cgagaa
//
LOCUS       DAOSWW010000997         1146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_118970_length_1146_cov_3.030247, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000997 DAOSWW010000000
VERSION     DAOSWW010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118970_length_1146_cov_3.030247"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PK442_14910"
     CDS             complement(<1..445)
                     /locus_tag="PK442_14910"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOO88995.1"
                     /translation="MKKLLALFLAIVLMTGFVAAANAADDPMKYEGRLSIASQNVNET
                     IILAWMAKLLVEEYTGLRTTMNTEFAGSAVLHQAMAAGEIDLYSSWTGTQLTGILRYE
                     GESKSSEETFRMVKEGFEKNFGMTWTRPVGFNNTYIMAVRPETAEK"
     gene            complement(502..>1146)
                     /locus_tag="PK442_14915"
     CDS             complement(502..>1146)
                     /locus_tag="PK442_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO88996.1"
                     /translation="WLTGEHLLISLIAVGITVAICVPLGIYLTRNERAAPYVIGLANV
                     FQTVPSLALLGFLIFVLGIGNDNAICALFLYAMLPVLQNTYTGIRGVPKSYVQAARGM
                     GMTDFQILMKVQFPLALPVLVAGIRVAMVWTIGTATLASAIGGCGLGRLIFSGLSSIR
                     NEIILAGAIPATLLALAADWLLKRAQEYFNPSARAKRLAKRHGISAETESNSAA"
BASE COUNT          220 a          352 c          359 g          215 t
ORIGIN      
        1 acttctccgc cgtctcgggg cgcacggcca tgatgtacgt gttgttgaag ccgaccgggc
       61 gcgtccacgt catgccgaag ttcttctcga acccctcttt caccatccgg aaggtctctt
      121 cggaggactt cgattcgccc tcgtagcgca gaatgccggt gagctgcgtc ccggtccacg
      181 aggaatagag gtcgatctcc ccggcggcca tggcctggtg aagcaccgcc gacccggcga
      241 actccgtgtt catcgtcgtg cgcagcccgg tgtactcctc gacgaggagc ttcgccatcc
      301 aggcgaggat gatcgtctcg ttgacgttct gcgacgcgat actcagccgc ccctcgtact
      361 tcatcggatc atcggccgcg ttggcggcgg cgacaaaccc tgtcatcagt actatcgcaa
      421 gaaatagtgc aagcaacttc ttcatggaac cactctcctt taagatattt tttacatcgt
      481 tcacggcgcg gagcgcggag ctcacgccgc ggagttggat tctgtctcgg cggaaatacc
      541 gtgcctcttc gccagccgct tcgcccgcgc ggaggggttg aaatactcct gcgcgcgctt
      601 cagcagccag tctgcggcga gcgcgagcag cgtcgcaggg atggccccgg caaggatgat
      661 ctcgttccgt atggatgaga gaccggagaa gatgagccgt ccgagtccgc agccgccgat
      721 cgccgaggcg agcgtcgccg tgccgatcgt ccagaccatg gccacccgga tgcccgcgac
      781 gagcacggga agggcgagtg ggaactgtac cttcatcagg atctggaagt cggtcatccc
      841 catgccgcgc gcggcctgaa cgtaggactt cggaacgcct ctgatgccgg tgtaggtgtt
      901 ctggagcacc ggaagcatgg cgtagaggaa gagcgcgcag atcgcgttgt cattgcctat
      961 tcccagaacg aagatcagga aacccaacag ggcaaggctc ggcaccgtct gaaagacgtt
     1021 cgccaggccg atcacgtacg gggctgcgcg ctcgttccgc gtgaggtaga tccccagtgg
     1081 aacgcagatg gcaacggtga tgccgacggc gatcagagag atgagcaggt gctctccggt
     1141 gagcca
//
LOCUS       DAOSWW010000998         1142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_119694_length_1142_cov_2.759890, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000998 DAOSWW010000000
VERSION     DAOSWW010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119694_length_1142_cov_2.759890"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PK442_14920"
     CDS             <1..342
                     /locus_tag="PK442_14920"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOO88997.1"
                     /translation="SRLAERGGVMGMNACSVFVADPDAKAVTERDLADHVDHIRSLVG
                     DGHVGFGFDFCDEIRDFAAPGPHREYDCIKGYGASVEFTAELLRRGYGEDAVRSIMGG
                     SFLAFLKQTIG"
     gene            430..>1142
                     /locus_tag="PK442_14925"
     CDS             430..>1142
                     /locus_tag="PK442_14925"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOO88998.1"
                     /translation="MSLFFVLLCAVCILFLLFPRASRACTSVVAGRNASSTGRVIVGH
                     NEDDPGRLIVRHALVPSRRWDDGDVITAEERSARIPQPPRTLAFFWTQVRGERGLSNA
                     DGFYNERGVLIVSDSCLSSRLDDEPDLVAGGIGYALRRAVAERASSARSGVEIAAQLV
                     DRYGYRSPGRGYVIADSKEAWIVQFAGGKDYCGVRVHDDEVVFFPNHFTIRAGDLEGH
                     ERILSPGLAEKALSRGWSA"
BASE COUNT          181 a          357 c          390 g          214 t
ORIGIN      
        1 tccaggctgg ccgagcgcgg aggggtgatg gggatgaacg cgtgcagcgt cttcgtcgcc
       61 gatccggacg cgaaggccgt gacggagcgg gatcttgccg accacgtcga tcacattcgt
      121 tcgctcgtcg gcgacgggca tgtcggcttc gggttcgact tctgcgacga gatacgcgac
      181 ttcgccgccc ccggaccgca tcgcgagtac gactgtatca aggggtacgg cgcgagcgtc
      241 gagttcactg cggagcttct gcgcaggggg tacggcgagg acgccgtccg cagtatcatg
      301 ggaggttcgt tcctcgcatt cttgaagcaa acgatcggat agactacccc ggccgcagcg
      361 aattcaggtt gtttggctct cgcccgaatg tccgagctgc ccccggatca accaaaggag
      421 ctggtgagcg tgtcgttgtt tttcgttctt ctgtgcgctg tgtgcatact gtttcttctg
      481 tttccccgcg cctcccgcgc gtgcacttcc gtcgtcgccg ggcgaaacgc ctcctcgacg
      541 gggcgcgtca tcgtcggcca taacgaggac gatccggggc gcctgattgt ccggcacgcg
      601 ctcgtcccct cccgtcgctg ggacgacggc gacgtcatca cggccgaaga gcggtcggcc
      661 agaatccctc agcctccccg gacccttgct ttcttctgga cgcaggtcag gggggagcgg
      721 ggcctttcga acgcggacgg tttctacaac gagcgggggg tgctgatcgt cagcgacagc
      781 tgcctctcct ccaggctgga cgacgaaccg gacctcgtcg cgggcggcat cgggtacgcg
      841 cttcgccgcg ccgtcgcgga gcgcgcttcg agcgcccgtt ccggggtgga gatcgcggcg
      901 caactcgtcg acaggtatgg ctatcgcagt ccgggcagag ggtacgtgat cgcggattcg
      961 aaagaggcgt ggatcgtcca gttcgcgggg ggaaaagact actgcggcgt gcgcgtccac
     1021 gacgacgaag tagtcttctt ccccaatcac ttcaccatcc gggcgggcga cctcgaaggg
     1081 cacgagcgca tcctgtcgcc ggggctggcg gagaaagccc tcagtcgagg atggagcgcg
     1141 ca
//
LOCUS       DAOSWW010000999         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_120107_length_1140_cov_2.525346, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010000999 DAOSWW010000000
VERSION     DAOSWW010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120107_length_1140_cov_2.525346"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PK442_14930"
     CDS             complement(<1..502)
                     /locus_tag="PK442_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOO88999.1"
                     /translation="MATLIQQIINGLSLGSVYALIAVGYSLVYSVLLFSNFAHGGFLV
                     IGGYICYAALRTFNIGIWMAGALSLVGAGAAAILTERLAYRPIRERTNVTLYLLIASM
                     GMSIVIENIFVIVAGGRFRALPPVIPTTPVNFFGLATTSAFDLLSLVSAVVFLGALQL
                     FLSKTRW"
     gene            complement(626..>1140)
                     /locus_tag="PK442_14935"
     CDS             complement(626..>1140)
                     /locus_tag="PK442_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO89000.1"
                     /translation="RAQLTEIRDKGADVLVLPNMGKEMALIMKQARELGMNEIVFVGG
                     DGYGEFMWEIAGEAMEGSYWINHVAPEDPAMAPFFAAYKEKWDDECKEFVNGVLGYDS
                     IYWVADAISRAGKADPKLIRDALEATKDLKLHHATITMDPKTHNPVDKDGVVLIAKDG
                     KGQFFTKIKP"
BASE COUNT          271 a          313 c          314 g          242 t
ORIGIN      
        1 cccatcgggt cttcgacagg aagagctgca atgcgccgag gaaaacaaca gccgaaacaa
       61 gggaaagaag atcgaacgcg cttgtagtgg caagaccgaa aaagttcacc ggcgtggtcg
      121 ggatcaccgg aggaagagcg cggaaacgtc ctcccgccac aatgacaaag atattttcga
      181 tgacaatgct cattcccatc gaggcaataa gaagataaag agtcacattg gtgcgttcgc
      241 ggatcgggcg ataggcgagc cgttccgtga gaatggccgc cgctcccgct cctaccagag
      301 aaagcgcacc cgccatccat attccgatgt taaaagtacg aagcgcagcg tagcagatat
      361 aaccgccgat tacaagaaag cctccgtggg cgaagttgga gaagaggaga acggaataga
      421 ccagagagta tcccacggca atgagtgcgt agaccgatcc aagcgagagg ccgttgatga
      481 tctgctgtat cagcgttgcc aaatatggat cacccctttc acgtattgac gagggcagac
      541 ggtctccgcc taccctcgtc atggaacaac tatatcatat ttttttcttc acggtatatt
      601 gtattattta aactctgcgg tgcacttacg gcttgatctt cgtgaagaac tgtcccttgc
      661 cgtccttggc tatgagaacg acgccgtcct tgtcgacagg gttgtgcgtc ttcgggtcca
      721 tggtgatggt ggcatggtga agcttcagat ctttggtggc ctcaagcgcg tcgcggatga
      781 gctttggatc ggccttaccg gcgcgggaga tcgcgtcggc cacccagtaa atagagtcat
      841 agccgagcac accgttcacg aactctttgc actcgtcatc ccacttttcc ttgtacgccg
      901 cgaagaaggg cgccatcgca ggatcctcgg gagcgacatg gttaatccag taggagccct
      961 ccatggcctc tcctgcgatc tcccacatga actcgccgta cccgtcgccg ccgacgaaga
     1021 cgatttcgtt cataccaagc tcgcgtgcct gcttcatgat gagcgccatc tccttgccca
     1081 tgttgggaag gacgagcaca tcggcgccct tgtcgcgtat ctcggtgagc tgcgcgcgga
//
LOCUS       DAOSWW010001000         1138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_120408_length_1138_cov_4.349030, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001000 DAOSWW010000000
VERSION     DAOSWW010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120408_length_1138_cov_4.349030"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PK442_14940"
     CDS             <1..505
                     /locus_tag="PK442_14940"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164424.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HOO89001.1"
                     /translation="NKPLMRKMPGRICGQTTDSQGRRTFVLTLQAREQHIRREKATSN
                     ICSNQNLCIVAASVYLSLMGPQGLRDIGGQILAKSAYAVEQLEKTGKFRRAFPGVPSF
                     RETALLCEEPVDKLNARLLQQGILGGYSLEAEYPRLKNGWLLAVTESRTKEEIDRLVA
                     VAGGDAR"
     gene            502..>1138
                     /locus_tag="PK442_14945"
     CDS             502..>1138
                     /locus_tag="PK442_14945"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520379.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOO89002.1"
                     /translation="MKRTMDTLFETSRPGRVGYALPELDVPAVDCGEELKGFCRAAAP
                     RLPELAEVDVVRHFTGLSKRNYSVDEGFYPLGSCTMKYNPKVNEVAARMPGFSRIHPL
                     QPEGTVQGALKLMYDLSAMLAEITGMDAVTLQPAAGAHGEQTGLMMIKAYHESRGDHK
                     RTKVIVPDSAHGTNPASAAVTGFDTVEVASDAEGMVDLDALTHPFGIEFACL"
BASE COUNT          216 a          388 c          367 g          167 t
ORIGIN      
        1 gaacaaaccg ctgatgcgga agatgccggg gcgcatctgc ggccagacca ccgacagcca
       61 agggcgccgc accttcgtcc tcacgctcca ggcgcgcgag cagcacatcc gccgcgagaa
      121 ggccacgtcg aacatctgct cgaaccagaa cctctgcatc gtcgcggcct cggtctacct
      181 cagcctcatg gggccgcagg gactccgcga catcggcggc cagattctcg ccaagtccgc
      241 ctacgcggtg gagcagctcg aaaagacggg caaattccgg agggcgttcc ccggcgttcc
      301 gtctttccgc gaaaccgcgc tcctctgcga ggaaccggtg gacaagctga acgcccgcct
      361 tctgcagcag ggcattctcg gcgggtactc cctggaagcg gaatatccgc gcttgaagaa
      421 cggctggctc ctcgcggtaa cggagagccg cacgaaagaa gagatcgacc gcctcgtcgc
      481 ggttgcggga ggtgacgccc gatgaaacgc acgatggaca cgctttttga aaccagccgc
      541 cccggacggg tcggctacgc gctgccggaa ctcgacgttc ccgccgtgga ctgcggcgag
      601 gagctgaagg gcttctgccg cgccgcggcc cccagactgc ccgagctggc cgaggtggac
      661 gtggtgcgcc acttcaccgg cctctcgaag aggaactact cggtcgacga ggggttctac
      721 cctctcggct cgtgcaccat gaagtacaac ccgaaggtga acgaggtcgc cgcccggatg
      781 ccgggcttct cgcgcatcca cccccttcag cccgagggaa cggtccaggg cgcgctgaag
      841 ctcatgtacg acctgagcgc gatgctcgcg gagatcacgg ggatggacgc ggtcacgctc
      901 cagcccgccg ccggagccca cggcgaacag accggcctga tgatgatcaa ggcgtaccac
      961 gagagccggg gagaccacaa gcggacgaag gtcatcgtcc ccgactccgc gcacggcacc
     1021 aacccggcgt cggcggcggt caccggcttc gatacggtcg aggtcgcctc agacgccgag
     1081 gggatggtcg acctcgacgc gctcacgcat ccttttggga tagaatttgc ctgtcttg
//
LOCUS       DAOSWW010001001         1138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_120432_length_1138_cov_2.880886, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001001 DAOSWW010000000
VERSION     DAOSWW010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120432_length_1138_cov_2.880886"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK442_14950"
     CDS             complement(<1..516)
                     /locus_tag="PK442_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOO89003.1"
                     /translation="MSARTTSPLLYFSAYAALYIIWGSTYLAIRFTVETIPPFLSGSL
                     RFFLAGGILLVWCLYREQERPTLRGFLAALKVAGIMLLGGYGGVVWAQQTVPSSMAAL
                     IISIEPLWFVIFDWLFFKSRKPSTAETIGVALGFGGTVFLVLSQSRYSLSLKGEYTVG
                     MLVEKDGAAESE"
     gene            848..>1138
                     /locus_tag="PK442_14955"
     CDS             848..>1138
                     /locus_tag="PK442_14955"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOO89004.1"
                     /translation="MLPKKMVDLDRVIASSPVVVLWGEPLDHRPVDFVTDNVSQFGYA
                     VPEMVLEEVRYDSIVFDEDRERVRSAFQNALLDGSDSFILDYRIITRAGAVRW"
BASE COUNT          256 a          297 c          346 g          239 t
ORIGIN      
        1 ctcggattcg gcggcaccgt ctttctcgac cagcataccg acggtgtact ctcccttcag
       61 cgagagcgag tacctcgact gcgagaggac gagaaagacg gtgccgccga atccgagggc
      121 gactccgatc gtttcggccg tagacggctt gcgcgatttg aagaagagcc agtcgaagat
      181 gacgaaccag agcggttcga tggagatgat gagggccgcc atggacgagg gaaccgtctg
      241 ctgcgcccat accacgccgc cgtaccctcc gagcagcatg atcccggcca ctttcaacgc
      301 ggcgaggaat cctcgcagcg tggggcgctc ctgttccctg tacagacacc agacgagcag
      361 aatgcccccg gcgaggaaga agcggagaga gccgctgagg aacggcggta tcgtttccac
      421 cgtaaagcgg atggccagat acgtggaacc ccagatgata tagagcgccg cgtaggcgga
      481 gaagtagagc agcggagacg tggtgcgagc ggacatgaga ataaccccca gaatgtattc
      541 gaatcgcggc gatattatag cagatagttc cttcttacaa catcctcgat tgaaaaaagc
      601 agatttttaa gagggaattg ccggtccggt gtttctcctc cgttccaacc tgcttcccgg
      661 cggcgcgctc ttcagtggcg agcgcgttgg ctgctctttt cgcccgcgga tttacgggtg
      721 aaatgctccc tgttcaaagc tgaagaattc gcgtaaaata aaaaatgcaa tgtgataagt
      781 tgattctttg tccgcatgcg tttttgtgct gttgacctgt tttttccaag agcgaaggag
      841 atggagcatg cttccgaaga agatggtcga tcttgatcgg gtgatcgcat caagcccggt
      901 ggtggtcctc tggggcgagc cgctggatca tcgtcccgtg gacttcgtaa cggacaacgt
      961 gagccagttc ggatacgctg tgcccgagat ggtgttggag gaagtccgat atgactccat
     1021 tgtgttcgac gaggacagag aacgggtgcg ttcagccttt caaaacgcat tgctggacgg
     1081 gagcgactcg tttatcctcg actatagaat catcacccgc gccggagcgg tccgctgg
//
LOCUS       DAOSWW010001002         1138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_120442_length_1138_cov_2.804247, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001002 DAOSWW010000000
VERSION     DAOSWW010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120442_length_1138_cov_2.804247"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1138)
                     /locus_tag="PK442_14960"
     CDS             complement(<1..>1138)
                     /locus_tag="PK442_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOO89005.1"
                     /translation="VLTGNTLTLDQLHAIARMNEPVEIHPDAARRLEESRKLVFDLVE
                     SNVPVYGFNTGVGWNKDKHIAADFFKEYNENVIHSHSLGVAPEASREEVRAAMAIRLN
                     CLLRGNTGIQPAIARRYAEFLNLGITPVVPERGSVGESDIAVLSHIGLAMIGEGDADY
                     KGTRMTSARAHAETGLQPVELGPKDALAIVNSSAFGMAQGAMVLCDLLDLVDQADLIY
                     AISLEGLNGNTSPLEQKVHEARRLQGQAKSAALVNRFLEGSFIFSPDPEKPVQDPLCF
                     RGGAYVHGSLRDALEYVVEKSLVQMNCSDDNPCLLLDERRIVSCSNFETTTLAAGFEM
                     LSVVLSHLSRMSCYRMIKLGNPAFTGLTRFLTPAEGKVHAFGTIQ"
BASE COUNT          207 a          332 c          365 g          234 t
ORIGIN      
        1 ctggatcgtt ccgaaggcgt gcaccttccc ttccgcgggc gtcagaaagc gcgtcagtcc
       61 ggtgaacgcc gggttgccga gcttgatcat cctgtagcag gacatccggg aaaggtggct
      121 cagcacgacg gaaagcattt cgaatccggc ggccagggtg gtcgtctcga agttcgagca
      181 cgagacgatg cgccgctcgt ccagcagaag gcaggggttg tcgtcggagc agttcatctg
      241 gacgaggctc ttctcgacga catactccag cgcatcgcgg agcgaaccgt ggacataggc
      301 tcctccgcgg aagcagagcg ggtcctgtac gggtttttcc ggatcgggag agaagatgaa
      361 gctcccttcc agaaaccggt tgacgagcgc ggcgctcttc gcttgccctt gaaggcggcg
      421 cgcttcgtga accttctgtt cgagcgggga tgtgttcccg ttcagtcctt cgagagagat
      481 cgcatagatc aagtcggcct ggtcgacgag atcaagcaga tcgcagagga ccatcgcacc
      541 ctgcgccatg ccgaaagcgc ttgaattgac gatcgcaagc gcgtccttcg ggccgagttc
      601 aaccggttgc agccccgttt cagcgtgggc gcgggccgag gtcattctcg ttcccttgta
      661 gtccgcgtcg ccttcgccga tcatcgcgag tccgatgtgc gagaggacgg cgatatcgga
      721 ctccccgacg gagccgcgtt cgggaactac cggggtaatg ccgaggttta agaactccgc
      781 gtaccgccgc gctattgcgg gctgtatgcc tgtgttgccg cgaagcaggc agttcaggcg
      841 gatcgccatc gctgcccgaa cctcctcgcg cgaagcctcg ggagcgacgc cgagggagtg
      901 cgagtggatg acgttttcgt tgtattcctt aaagaagtcc gccgcgatat gtttatcctt
      961 gttccacccc acgccggtat tgaatccgta caccggtacg ttgctctcga cgaggtcgaa
     1021 aacaagcttt cgggactcct ccaggcggcg cgcggcgtcg gggtgaatct ccacgggctc
     1081 gttcattctg gcgattgcgt gcagctgatc caacgtgagg gtgtttccgg tcagtacg
//
LOCUS       DAOSWW010001003         1137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_120567_length_1137_cov_4.131238, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001003 DAOSWW010000000
VERSION     DAOSWW010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120567_length_1137_cov_4.131238"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PK442_14965"
     CDS             <1..198
                     /locus_tag="PK442_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOO89006.1"
                     /translation="LLDLGVQNIRLLTNNPRKVVGLQGYGLEIAERIPLVIPPNKFNE
                     RYLGTKEKKLGHMLHICDLLR"
     gene            227..718
                     /gene="ribE"
                     /locus_tag="PK442_14970"
     CDS             227..718
                     /gene="ribE"
                     /locus_tag="PK442_14970"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOO89007.1"
                     /translation="MRVIEGKLTGAGLRFCIVVSRFNSFFSEKLLEGAKDALLRHDVS
                     HHGIDIVWVPGAWEIPLIAKEAALSGKYDAIIALGAVIRGDTTHYEHVCAEMSKGIAS
                     VSMTQRVPVSFGVLTCESMDQAVARSGSKAGNKGFEAAAAAIEMANILKQMRTEKETD
                     QNA"
     gene            711..>1137
                     /locus_tag="PK442_14975"
     CDS             711..>1137
                     /locus_tag="PK442_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOO89008.1"
                     /translation="MLDIRWIRSNADEVKAYLADRNNDFDIEPLLALDEEKRKLLSET
                     EELKAKRNEGSRKVGMAKSKGEDAGELMEEMRVIGDRIRDIDSTLAEIEEKMNNMILS
                     IPNRPHESVPVGNDENDNPVVRRWGEPKSFAFEPKAHWDI"
BASE COUNT          288 a          272 c          361 g          216 t
ORIGIN      
        1 cttctggatc ttggggtaca aaacatcaga ctgctgacga acaatcccag gaaggtggtc
       61 ggtcttcagg ggtacggact tgagatcgcg gaacggatac cgctggtgat tccgcccaac
      121 aaatttaacg agcgctacct tggaaccaag gagaaaaagc tcggccacat gctgcatata
      181 tgcgatcttc tgcggtaagg gagatcctga agggagagag ttccgaatga gggttatcga
      241 aggaaaactg acgggggcgg ggcttcggtt ctgcatcgtt gtttcgcgtt tcaacagctt
      301 cttcagcgag aagctgctcg aaggagcgaa ggatgcgctg ctgcgccatg atgtatctca
      361 tcacggcatc gacatcgtat gggttccggg agcgtgggag atccccctga tcgcgaaaga
      421 ggccgcgctg agcggcaagt acgacgcaat tatcgcgctc ggcgccgtga tacgaggcga
      481 tacgacgcac tacgaacacg tctgcgcgga gatgtcgaaa ggaatcgcgt ccgtcagcat
      541 gacgcagcgt gtgccggtgt cgttcggcgt tttgacgtgc gagtcgatgg atcaggccgt
      601 cgcccgttcg ggaagcaagg ccggtaacaa agggttcgag gcggcggcgg ccgctatcga
      661 gatggcgaat atattgaagc agatgcggac tgaaaaggag actgatcaga atgcttgata
      721 tccgatggat tcgttccaac gccgatgaag tgaaggccta cctggccgac agaaacaacg
      781 acttcgatat agagcctctt cttgcgctcg acgaagagaa acggaaactg ctctccgaga
      841 ccgaggaact gaaagcgaag cggaacgagg gctcgcgcaa ggtcggtatg gcgaagagca
      901 agggggagga tgcgggcgag ctgatggaag agatgcgcgt catcggcgac aggatccgcg
      961 atatcgatag tacgcttgcg gaaattgaag agaagatgaa taacatgatc ctctccattc
     1021 ccaatcgtcc gcacgaatcg gtccccgtag gaaatgacga gaacgacaat ccggtagttc
     1081 gccgctgggg agagcccaaa tcttttgctt tcgaaccgaa agcgcactgg gatatcg
//
LOCUS       DAOSWW010001004         1135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_120950_length_1135_cov_4.231481, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001004 DAOSWW010000000
VERSION     DAOSWW010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120950_length_1135_cov_4.231481"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /gene="nrdR"
                     /locus_tag="PK442_14980"
     CDS             complement(<1..204)
                     /gene="nrdR"
                     /locus_tag="PK442_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOO89009.1"
                     /translation="MRCPSCGALETRVIETRTADEGRVVRRRRECPECSTRFTTYEKI
                     EEKRPLRVIKKDGSRETFGRDKIV"
     gene            complement(379..843)
                     /locus_tag="PK442_14985"
     CDS             complement(379..843)
                     /locus_tag="PK442_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOO89010.1"
                     /translation="MIELEKRLSDDLVAAMKSRKEMELSSLRMLKAEVQRAKAEKGRS
                     VELTEDDIVALIQRLVKQRREAADQYTLVGAGDRAAEELREALFLETYLPEQLSDEEL
                     EHIVEEAGAFVKAAGPKDMGKVMGKVMAAVKGRADGKRVRSKVHDYLQTLVE"
     gene            complement(850..1038)
                     /gene="rpsU"
                     /locus_tag="PK442_14990"
     CDS             complement(850..1038)
                     /gene="rpsU"
                     /locus_tag="PK442_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOO89011.1"
                     /translation="MTTVVRRDNESLDDALRRFKREVSKVGTLREVRKREHYEKPSEA
                     KKTKRAEAARKRRSKPRG"
BASE COUNT          225 a          362 c          255 g          293 t
ORIGIN      
        1 gacaatcttg tcccgaccga acgtctccct gctgccgtcc ttttttatca cccggagagg
       61 acgtttctcc tcaatttttt catatgttgt gaaccgagtg gagcactccg gacactccct
      121 cctccggcgg accacacgcc cttcatccgc agtcctcgtc tcgattaccc gcgtctccaa
      181 agcgccgcat gacggacatc gcacagacac accccctata tatagcaatc accgcgcaat
      241 actactccta tatggtgtat ctttccagag agacgttgta ctcctttcat acgggacttt
      301 aagacgtact gcaaagcaca ataaaaaaag ccgggtttcc ccggcttttc taggtctcaa
      361 acaattgtgt tgcaacggtc attcgacaag ggtctgaaga tagtcatgca cctttgaacg
      421 aactcgttta ccgtcggcgc ggccttttac ggcagccatg accttcccca tcacttttcc
      481 catatccttg ggtccggcgg ctttgacaaa cgcgcccgct tcttcgacaa tgtgttccaa
      541 ttcttcgtcg gagagctgct caggaagata ggtttcaagg aaaagagctt ccctcagttc
      601 ttcagccgcc ctgtctcctg caccaaccaa tgtgtactgg tcggcggcct cacgccgttg
      661 tttcacaagg cgctgaataa gggcgacaat gtcgtcctcc gtcagttcca ctgaacgccc
      721 cttctcggct ttggcccgct gcacctccgc cttgagcatc cggagagatg agagttccat
      781 ctctttccgg gatttcatgg cggcgacaag atcatccgat aaacgcttct cgagttcaat
      841 cacgcggcat taccccctgg gcttgctccg tcttttcctg gcggcttcgg cccgcttcgt
      901 ctttttcgcc tcactgggct tttcgtagtg ctccctcttc cggacctccc gaagggttcc
      961 caccttggag acttcccgtt tgaaccgcct gagggcgtca tcgagggatt cgttatctct
     1021 tcgaacgaca gtcgtcataa acattccccc ctttctttgc cacccgcatc taccccggtg
     1081 gccagcggaa actacgtccc gccaacaggt gaatatgaag gtggggaatc gtctg
//
LOCUS       DAOSWW010001005         1135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_121017_length_1135_cov_2.453704, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001005 DAOSWW010000000
VERSION     DAOSWW010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121017_length_1135_cov_2.453704"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PK442_14995"
     CDS             <1..184
                     /locus_tag="PK442_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89012.1"
                     /translation="ANSARTVQSGARLLSFENGVATLSPGSERIELSLAELENRSAYI
                     VGMRRSREVEAAPQVK"
     gene            complement(469..>1135)
                     /locus_tag="PK442_15000"
     CDS             complement(469..>1135)
                     /locus_tag="PK442_15000"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HOO89013.1"
                     /translation="LMRMAQLACRHTQYPELSPGMMLVEHRRCVPEIIAYCNDLVYKG
                     KLEPRRRESEEERRSRPSFLPAMGEIPTVSDDERASGSRRNPEEAARIAEWTKEHLKE
                     LETRYEKPIGEILGIVTPFAAQKKAVRKALADALGDDRGVVTGTVHALQGAERDVVLF
                     SPVYGRGHSGNTFFDAGPNMMNVAVSRARDSFIVVGNPDLFVEGSGKPSGILARHLRK
                     PSL"
BASE COUNT          194 a          367 c          347 g          227 t
ORIGIN      
        1 tgcgaactcg gcgagaacgg ttcagagcgg cgcgaggttg ctctctttcg agaatggggt
       61 cgcgacgctc tcacccggct cggagaggat cgagctctcc ttggcggagc ttgaaaacag
      121 gagcgcgtac atcgtcggga tgcggaggag cagagaggtc gaggccgcgc cgcaagtgaa
      181 atagaacacc gtactgccgc tacccgcgca gcgaatcttc tatgttctcg ggcaacaccg
      241 gaatcaatac gtcattcaat ttccgtcctt ctacccgaac cgcacgcgcg cggagacagc
      301 cttccgttct ttccggagac gctcggctgg ggctgcttgt tctccgccga agagaagttc
      361 ggtgaagcac caccctcccc cttcgctgaa ttcttccgga agagttcctt cgacgcatcc
      421 tttctcggag gactcatgat ctcttccctt ctcgaagcga agcgcgcgtc aaagggatgg
      481 cttccgcaga tgccgcgcca gaatcccgga tggcttaccg ctcccctcca cgaagagatc
      541 cggattcccg acgacgatga aggagtcccg cgcgcgcgat acggcgacgt tcatcatgtt
      601 cggccccgcg tcgaagaagg tgttcccgga gtgtccgcgg ccgtagaccg gggagaagag
      661 caccacgtcc ctctcggctc cctgcagggc gtggaccgtg ccggtcacga cgccccggtc
      721 gtcgccgaga gcgtcggcga gcgccttccg gacggctttc ttctgggcgg cgaagggcgt
      781 gacaatcccc agtatctccc ctatcggctt ctcgtacctc gtttcgagct ccttcagatg
      841 ctccttcgtc cactcggcta tcctggcggc ttcctccggg ttcctgcggc ttcccgaggc
      901 gcgctcatca tcggagaccg tcgggatctc ccccatcgcc ggcaggaagg aggggcggct
      961 tcgtcgctcc tcctcgctct ctcttctgcg cggctcgagc tttcccttgt agacaaggtc
     1021 gttgcagtag gcgatgatct cagggacgca ccgacggtgc tcgacgagca tcatgccggg
     1081 ggagagttca gggtactggg tatggcggca ggcgagctgc gccatgcgca tcaga
//
LOCUS       DAOSWW010001006         1134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_121149_length_1134_cov_3.102873, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001006 DAOSWW010000000
VERSION     DAOSWW010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121149_length_1134_cov_3.102873"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PK442_15005"
     CDS             complement(<1..803)
                     /locus_tag="PK442_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89014.1"
                     /translation="MTEGETASEDIADRVLPEAETEVLSGDVSVPRAASEDIEKAEAG
                     WGTLSVSLLPEGARTAGARWSADGGKTWRASGEKILLANADTTVTFKSLVGWKTPETI
                     PVRISNESGAQAAATYVQLTGSIRAEIDGPEGARWSINGKGEFLSGNMREGLVPGDYT
                     VSFSDVVGWLKPENASVSVSSGATAAVSAAYVRTGSVRVTINGPAEARWTIGEKINLP
                     SGTLIEGLAPGEYTISFSEVALWNKPEDLTITLASGALLRAGASYAAKNG"
BASE COUNT          233 a          350 c          312 g          239 t
ORIGIN      
        1 ccgttctttg cggcgtaaga ggctccagcg cgcagaagtg ctccggaagc gagcgtaatc
       61 gtcagatctt ccggtttgtt ccatagcgct acttccgaga aggaaattgt gtactctccg
      121 ggagcgagtc cttcgatgag cgtgccgctc gggaggttga ttttttcccc gatggtccat
      181 ctggcttcag caggtccatt gattgtgacg cggacggaac ccgtacggac ataggctgcc
      241 gatacggctg ctgtggcgcc tgaactgacg gagacgctcg cgttctccgg tttcagccac
      301 cccacgacgt ccgaaaacga gacggtatag tccccgggaa caagcccttc gcgcatattt
      361 ccgctgagga actcgccttt gccgttgata ctccatcgcg ctccctccgg tccgtcgatt
      421 tccgcccgga tggaacctgt gagctgaacg tatgtcgcgg ctgcctgcgc accgctctcg
      481 ttgctgatcc ttacgggaat agtctccgga gtcttccagc cgacgaggct cttgaaggtc
      541 accgtcgtgt cggcgttggc aaggagaatt ttctccccgg aagctctcca tgttttgcct
      601 ccgtcggcgc tccagcgcgc ccccgcagtc cgcgcgcctt caggcagcag cgaaacggaa
      661 agcgttcccc accccgcttc ggctttttcg atatcctcgc ttgctgcccg cggaacggat
      721 acatctccgg aaagaacctc cgtttcggcc tccggaagaa cgcggtcggc gatatcctcg
      781 ctcgccgtct ctccttcggt caacagctgc ggaacggccg aacccgcgga cggagagggt
      841 gaaaccaaca aaacataaca gagcatcaaa aaacaacaaa tgtgccgcaa gcccagcctc
      901 acggtacttt tttcatgcgc cggcattcga tcatcccctt tcgatgtcgg ctggacatct
      961 cccccgagac atcctcccct gaactgcaag tgtacgatga gtgaaacggc ctgtcaacag
     1021 aaatcgcaag gcttgaattg caaggttgca tgccggaaaa agcgcgttac ggcggaaaga
     1081 acacgctctc cggaacaata ggttccggag agcgcagtga agtgcatcat gagg
//
LOCUS       DAOSWW010001007         1133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_121335_length_1133_cov_3.393321, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001007 DAOSWW010000000
VERSION     DAOSWW010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121335_length_1133_cov_3.393321"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1133
                     /locus_tag="PK442_15010"
     CDS             <1..>1133
                     /locus_tag="PK442_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOO89015.1"
                     /translation="SYVDGLSLFVHRLRDYFDADIAIAAVKMDTRTYLVGRSSEDALD
                     MAAFLTPFGGGGHPQAASVTLHNAKPLPLVREMEQLLHDAVKPSLTAGDIMTSPVMAV
                     PPDSLVDDAYRTMLRYGHSALPVVKGKNILGLITRKDLDKAHLHGFGKTLIREFMTEG
                     VITVPCDASIREAHRLLATHSIGRLPVVDGRRIMGIITRTDLVKALYPLSLPKEERET
                     TPELPWMEDVTTLLDEQLPPEQNKILALLGERAEKLGMRAYIVGGIVRDLLLRRENLD
                     LDIVVEGDAIRFLKSFEKDGFRVSVHERYKTGTLSFPRGVKVDVATARREFYEFPVAQ
                     PKVFSDSLKHDLYRRDYTVNAMAISINQSTRGVLVDYFGGRMD"
BASE COUNT          248 a          311 c          317 g          257 t
ORIGIN      
        1 catcgtacgt ggacggactc tcgctcttcg tccaccgtct cagggactac ttcgacgccg
       61 acattgccat tgccgcggtc aagatggata ccaggacata ccttgtcgga cgaagctcgg
      121 aggatgctct cgacatggcg gcttttctca cgcctttcgg gggaggagga catcctcagg
      181 ctgcatccgt gacgctgcat aacgcaaagc cgctgccgct tgtcagagag atggaacagc
      241 ttttgcacga cgctgtcaaa ccatcgctca ccgcaggaga catcatgacc tcgccggtta
      301 tggcggtgcc tccggattct cttgtggacg acgcctacag gacaatgctc cgctacggcc
      361 attccgcgct ccccgtagtc aaggggaaaa atatcttagg tttgataacc cgtaaggatc
      421 ttgataaggc gcaccttcac ggtttcggca agacgctgat tcgcgagttc atgacggagg
      481 gagttatcac cgttccgtgc gacgcttcta tccgcgaggc ccatcgtctc ttggcgacgc
      541 atagtatcgg caggcttcct gtagtggacg gtcgacggat tatgggcatt atcacacgga
      601 cggatctcgt caaggccctg taccccctct ccctccccaa ggaagaacgg gagacgactc
      661 cggagctccc atggatggag gacgtcacga cgcttctgga cgaacagctt ccgccggagc
      721 agaacaagat tcttgcgctt ctgggggagc gggcggagaa gctgggtatg cgtgcctata
      781 tcgttggagg gattgtccgc gatcttcttc tgcggagaga aaaccttgat cttgatatcg
      841 tcgtcgaagg agatgcgata cgtttcctca agagctttga aaaagacggt ttcagagtct
      901 cggttcacga acgatacaaa acgggtactc tttcatttcc ccgcggcgtc aaggtggatg
      961 ttgccaccgc acgcagagaa ttctacgagt ttcctgtagc ccagcccaaa gtcttcagcg
     1021 actctctgaa acacgacctg taccggagag attatacggt gaatgccatg gcgatctcca
     1081 tcaatcaatc aacgcgagga gttttggttg attatttcgg gggccgcatg gat
//
LOCUS       DAOSWW010001008         1132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_121518_length_1132_cov_3.863510, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001008 DAOSWW010000000
VERSION     DAOSWW010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121518_length_1132_cov_3.863510"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PK442_15015"
     CDS             complement(<1..306)
                     /locus_tag="PK442_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89016.1"
                     /translation="MKKDGLTAKKNGAFRNMKLGSRIILAVGALLLPAILIFAGIVLR
                     RIDADATASIEALRVSETERIRSDLRGYVDIAWRILSDKYEQSTQTESIIQSYGEQLR
                     "
     gene            complement(332..>1132)
                     /locus_tag="PK442_15020"
     CDS             complement(332..>1132)
                     /locus_tag="PK442_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO89017.1"
                     /translation="KRLVDLMGGELAVRSTLGEGSCFTASVPVEPSEETPAPPKTPPR
                     RVAGVERNEAEGPLLIVDDVKENRDILKALLAPLGFALREAENGREALRLFEKERPCA
                     VLMDMRMPVMDGYEATRRIKASEGGSSVPVIAITASAFEDDVQNVLKSGADICIRKPL
                     SPEDVYEALGKLLGIRFLYADEPRTADTEQEPPPSMEALAALPEELIKAMRRAVEDGD
                     IDELHTLVDRVREMNREVAEGLQKLVRQYDYAALSSLLSDGDRESGSS"
BASE COUNT          181 a          361 c          346 g          244 t
ORIGIN      
        1 gcggagctgc tctccgtacg actggatgat agattcggtc tgcgtcgact gttcgtactt
       61 gtcggagaga atgcgccatg cgatgtcgac gtagccgcgc aggtcggagc ggatgcgttc
      121 ggtctcggag actcgcagcg cctcgatgga ggctgttgcg tcggcgtcga tgcggcggag
      181 gacgattccg gcgaagatga ggatggcagg caacagcagc gccccgacgg ccagaatgat
      241 tcggctgccg agcttcatgt tccggaacgc gccgtttttc ttagcggtca atccgtcctt
      301 tttcattcga gttcctcctg gctgcgcccg atcatgagct tccgctctcc cggtcgccgt
      361 ccgagagaag agaagaaagc gccgcgtagt cgtattggcg tacgagcttc tgcaaccctt
      421 cggcaacctc ccggttcatc tcccgcaccc ggtcgacgag cgtgtgcagt tcgtcgatat
      481 cgccgtcttc gacggccctt ctcatcgcct tgatcaactc ttccggcagc gcggccagcg
      541 cttccatgga aggcggaggc tcttgttcag tatcggctgt ccgcggctcg tcggcgtaga
      601 ggaagcgtat gccgagcagc tttcccagcg cctcgtacac atcctcgggg ctgagcggtt
      661 tgcgtatgca gatatcggcg ccgctcttca gcacgttctg aacgtcgtcc tcgaaggcgc
      721 tcgccgtaat cgcgatgacg ggaaccgaac ttcccccttc gctcgccttg attctccttg
      781 tggcttcata gccgtccatc accggcatcc gcatatccat cagcacggcg cagggacgtt
      841 ctttttcgaa gagacgaagc gcctcccggc cgttttccgc ctctctgagg gcgaagccga
      901 gaggagcgag cagcgccttc aggatatcgc ggttctcttt gacgtcgtcg acgatgagca
      961 acggcccctc cgcttcgttg cgttccacgc ccgcgacgcg gcgcggcggc gtcttcgggg
     1021 gggcgggcgt ttcctccgac ggttcgaccg gtaccgacgc ggtaaaacag cttccctcgc
     1081 cgagagtgct tcgaacggcg agctctccgc ccatcaggtc gacaaggcgc tt
//
LOCUS       DAOSWW010001009         1132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_121526_length_1132_cov_3.589601, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001009 DAOSWW010000000
VERSION     DAOSWW010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121526_length_1132_cov_3.589601"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..>1132)
                     /locus_tag="PK442_15025"
     CDS             complement(170..>1132)
                     /locus_tag="PK442_15025"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO89018.1"
                     /translation="ECLPYYVTLAGGHGAGAEYVLRAEAALFRGDDVEAEVMCHTALF
                     EARSAGQIGNCLCASLVLARIMLLRGDGDSYTSIRRSILKDAREAQQTSLLRIGDLCL
                     AVTDLALGRLDDLPPWLRDAESIRKVVYTDGQPYAMMLHGMALLQEERYSELYGIAEP
                     VLEFARRMHYMLPQVYYNIYLAVAKNAEACREEAVGYLEQALLLALPDEVYLPFAEHG
                     DSLTPLFDSLQNAFPEDRVRAVRSLCERQVSGAEAVRKTLSGSGSILSARQREVALLL
                     KDGLSVKEISKNLFVTENTVKSITKAVYGKLGVHSRVELTVTSF"
BASE COUNT          222 a          328 c          344 g          238 t
ORIGIN      
        1 cggcgtcttt ccttccgtct ctttcgcaaa taaaggcatc aaggggcgac tgagcgtctc
       61 ttccaaggac gatacactgc tttcaaggtt tgtggaatca cccgttcggg tggtattccc
      121 ggggggattc cctggaagga cacgggaggg agcggttgaa tcgtgttttt caaaaggaag
      181 ttactgtcaa ttcgacgcgg gaatgcactc ccagctttcc atagacggcc tttgttatgc
      241 tcttcaccgt attttcggtt acgaagaggt ttttcgagat ctccttgact gaaaggccgt
      301 ctttcagaag aagcgctacc tcgcgctgcc tggcgctgag aatggatccc gaccctgaaa
      361 gggttttgcg gaccgcttcc gcgcctgata cctgacgttc gcacagcgaa cgcacagcgc
      421 gtacccggtc ttcgggaaaa gcgttttgaa gcgaatcgaa cagcggcgtc aacgagtctc
      481 cgtgttctgc gaaaggcagg tagacttcat ccggcagcgc cagtaggagc gcctgttcaa
      541 gatacccgac ggcctcttcc cggcaggctt cagcgttctt cgcgacggcg agatagatgt
      601 tgtaatagac ttgcgggagc atgtagtgca tcctgcgggc gaattccagg accggttccg
      661 cgatgccgta gagttctgaa tagcgctctt cctgaagcag cgccattccg tgaagcatca
      721 tcgcatacgg ctgaccgtcg gtgtacacca ctttccggat gctctcggcg tcgcggagcc
      781 agggaggcag atcgtcgagg cgtccgagcg ccaggtcggt aacggccagg cagaggtccc
      841 cgatgcgcag gagcgacgtc tgctgcgctt cccgggcgtc cttcagaatg ctgcgacgta
      901 tggaggtgta cgaatcgccg tcgccgcgca gcagcatgat acgggccagc accagcgatg
      961 cgcagagaca gttgccgatc tgccccgcgc tgcgcgcttc aaaaagcgcg gtatggcaca
     1021 tgacctccgc ttcgacatcg tctccgcgga agagcgcggc ctccgcgcgc aggacatact
     1081 ctgcgccggc gccgtgccct ccggcgaggg tgacgtaata cggcaggcat tc
//
LOCUS       DAOSWW010001010         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_121961_length_1130_cov_2.833488, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001010 DAOSWW010000000
VERSION     DAOSWW010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121961_length_1130_cov_2.833488"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..>1130)
                     /locus_tag="PK442_15030"
     CDS             complement(157..>1130)
                     /locus_tag="PK442_15030"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOO89019.1"
                     /translation="SILPPELERAVFLNSGSEAVELALKMAHAATGKTGVVVAEKGYY
                     GGTVYALSLSEAGRTASYLPKAGNVLRVAAPHCVRCDLSAECGDGEFPCMNVLEDLSP
                     EEWPAAILYEPLLGGGILVPPIGYGARLKEFARSLGALFISEEVTTGMGRTGKWFGFQ
                     HENIVPDILVVGKAIGGGLPVSAVVTTKEVEERAVPVLGRHVQSHQNTPFSGRIAAEV
                     IGAIRDEGLVERSERMGEYLLSGLREIQERFLCIREVRGRGLMVGAELTPEAASRGAG
                     MSKRLVEKGFLQDFHQLTSTFRLYPPFVITKDEIENFLEAFENVLAE"
BASE COUNT          251 a          355 c          304 g          220 t
ORIGIN      
        1 agggcagccc tgcaaaaagg aaattcataa agctcatttc gtgagtgcga ggcggttacg
       61 aatgccccgc actcacgaac ttacatcgcc tccgcgacaa tctgttacca agagagcatg
      121 ttccgcgtaa ggtgcaacag aggggcgctg aagcatctac tcggcaagca cgttttcgaa
      181 ggcctccaaa aagttttcta tctcgtcttt tgttatcaca aaaggagggt agagccggaa
      241 cgtcgacgtc aactggtgaa agtcctggag aaaccccttt tccacaagac gtttcgacat
      301 tccggcgccc ctggaggcag cctccggcgt cagctccgcg ccgaccatga ggcctctgcc
      361 gcgcacttca cggatacaga ggaaccgttc ctgtatctcc cggagccctg aaagcagata
      421 ttctcccatt ctctcggacc gttcgaccaa gccttcgtca cgaatcgcgc cgatcacttc
      481 cgcagcgatt ctcccggaga acggcgtgtt ctgatgcgac tggacgtgcc gtcctaaaac
      541 aggaaccgcc cgctcttcca cctctttcgt cgtgacaacc gccgagacgg gcagtccgcc
      601 gcctatggcc ttaccgacga ccaggatatc aggcacgata ttttcatgct ggaagccgaa
      661 ccacttgcca gtccggccca ttccggtggt cacctcctcg gagatgaaga gcgccccgag
      721 agaacgggcg aactctttga gccgtgctcc gtagccgatg ggcggcacaa ggatgccgcc
      781 tccgaggagc ggttcgtaga gaatcgccgc aggccattct tcaggcgaca gatcctcaag
      841 aacattcata cagggaaact cgccatcgcc gcactccgcc gagagatcgc accggacgca
      901 gtgcggcgcc gcgacgcgga ggacgtttcc cgcttttggc agatatgaag cggtccgccc
      961 agcttcggag agcgagagcg cgtaaactgt gcctccgtaa taccccttct ccgcgacgac
     1021 gactccagtc tttcctgtcg cggcgtgcgc cattttgagg gcaagctcca cggcttcgct
     1081 tccggaatta agaaaaaccg ccctttcgag ttccggagga agaatcgaag
//
LOCUS       DAOSWW010001011         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_121972_length_1130_cov_2.693023, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001011 DAOSWW010000000
VERSION     DAOSWW010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121972_length_1130_cov_2.693023"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PK442_15035"
     CDS             complement(<1..293)
                     /locus_tag="PK442_15035"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOO89020.1"
                     /translation="MRGFALRKFVAPEILFGRGALSLAGQYARNLGGGRVFVATDPGV
                     IEAGWLEPVLETLEQEELNYTVFSDIRPNPTASMVMAGARIFADERCDLILALG"
     gene            complement(386..>1130)
                     /locus_tag="PK442_15040"
     CDS             complement(386..>1130)
                     /locus_tag="PK442_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOO89021.1"
                     /translation="PATACVVQDRLGLKKTTGALDFNLGCSGFVYGLALAKGLIGTGI
                     AKKVLLITADTLSRTVHPMDKSTRTIFGDAAAATLVEGSDTARIGDFVLGTDGSGMDK
                     LIIPAGAWAAPRSPETAVERTNKWGNTRSAENMYMNGPEVLKFTVETVPPAVQQILEM
                     HSLKLEDIDLFVFHQATKLILEHLRKEIGIPEEKFYTNIENKGNTVSATIPMALRDAA
                     DEGRLRPGDRVMVVGFGVGFSWGATILRW"
BASE COUNT          206 a          374 c          319 g          231 t
ORIGIN      
        1 cccagggcaa gaatcaggtc gcaccgctcg tccgcgaaga tgcgcgcccc cgccatgacc
       61 atcgaggccg tgggattcgg ccgaatatcg gagaagacgg tgtagttcag ctcctcctgt
      121 tcgagagtct caaggacggg ttccagccag cccgcttcga tgactccggg atccgtggcg
      181 acgaaaaccc tgccgccgcc gagattccgg gcgtactgac ccgcgaggga gagggcccct
      241 ctcccgaaga gtatttcagg ggcgacgaac tttctcaggg caaaaccgcg catactctca
      301 tccccttttg tcacaataaa ggcgggggtg tattcccccg cctttatttt atcacaaaaa
      361 tggaataatg ttgcggtact ctcccttacc agcggagaat cgtcgctccc caggagaagc
      421 cgacgccgaa gccgacgacc atgactctgt cgccggggcg caatcttccc tcgtcggccg
      481 cgtcgcgcag ggccatcgga atggtcgcgc tcacggtgtt ccccttgttc tcgatgttgg
      541 tgtagaactt ctcctccgga atgccgatct ccttgcggag gtgctccaga atcagcttcg
      601 tcgcctgatg gaagacgaag aggtcgatgt cctcaagttt caaagagtgc atctcaagaa
      661 tttgttgcac tgcgggcgga actgtctcca cagtgaactt cagcacctcg gggccgttca
      721 tgtacatgtt ctccgcgctc cgcgtattgc cccacttgtt cgtccgttca acggccgtct
      781 ccggtgatcg cggggccgcc cacgccccgg ccggaatgat cagcttgtcc atccccgagc
      841 cgtccgttcc cagcacaaaa tcccctatgc gcgcagtgtc cgaaccttcg acgagcgtcg
      901 ccgcggcggc gtcgccgaag atcgtgcgcg tgctcttgtc catcggatgg acggtgcgag
      961 acagcgtgtc cgccgtgatc agcagcacct tcttcgcgat ccccgtccct atcagtccct
     1021 tcgcgagggc gagcccgtag acgaaccccg agcagcccag gttgaagtcg agcgcccccg
     1081 tcgtcttttt cagccccagt ctgtcctgca ccacgcaagc ggttgccggg
//
LOCUS       DAOSWW010001012         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_122037_length_1130_cov_1.972093, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001012 DAOSWW010000000
VERSION     DAOSWW010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122037_length_1130_cov_1.972093"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..1015
                     /locus_tag="PK442_15045"
     CDS             266..1015
                     /locus_tag="PK442_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89022.1"
                     /translation="MLNDRFDASKITGCISKDRLDVCARLLRVSSAEESVYCYHHLQT
                     LSSLCFIPLHYVEIVLRNKVHEALTAFYTGRKKSVALPGAPEEWYLWMPQRKKTIDVI
                     QESIQRLRRGKSSPVAGDIIAQLPFGVWVSVLEELPDEKEPLYFWRGIKHAVFSNLKT
                     PAGRRMILSELRDINNIRNRLFHHEPIWKTASVSSLRNALDEVRRKHEKMIAMLSWLS
                     ADMEALLRHVRHDDRFNSEYERIPSFYGRFV"
BASE COUNT          261 a          284 c          340 g          245 t
ORIGIN      
        1 tgcgttatct tggattcacg gacgacgagg tgcgcgcggc gtacgattcc gtcgccgcgt
       61 tgctgaccgg aggggaggaa aagcaatgaa gaacgatatc ggccgtgcgg agcataagaa
      121 aaggacctcc cttgcgggag gccctcattc tcgctttcag actttttact ccgtcctcgc
      181 gggcgaagca gccaactctg aagctcacat tgatatctag ggttattata tcaaaaaaga
      241 gttcggtgta aaggagctgt tccatttgct gaacgatcgt ttcgacgcgt cgaagataac
      301 gggctgcatc tccaaagacc ggctcgacgt ctgcgcgcgc cttttgaggg tgtcttccgc
      361 cgaggagtcg gtgtactgct atcatcatct tcagacgctc tcttcgctct gtttcattcc
      421 actgcactat gtcgagatcg tgctgcggaa caaggtgcac gaggcgctta ccgcttttta
      481 cacggggcgg aagaagtccg tggctctgcc gggcgctccg gaggagtggt atttgtggat
      541 gccgcagaga aagaagacca tagatgtgat acaggaaagc atccaaaggc tgcgaagggg
      601 aaagtcctct cctgtcgcgg gcgatatcat cgcgcaactt cctttcggag tctgggtcag
      661 cgtcctggag gaactccccg acgagaaaga gcccctgtac ttctggagag ggatcaagca
      721 cgcggttttt tccaatctga agacgccggc gggccgacgt atgatcctct ccgagctgcg
      781 ggatatcaac aacatcagga acaggctgtt tcatcacgag cctatctgga aaactgcgtc
      841 cgtctcttct cttcggaacg ccctcgacga ggtgcggaga aagcatgaaa agatgatcgc
      901 gatgctctcc tggctgtccg ccgatatgga ggcgctcctg cgccatgtgc ggcatgacga
      961 tcgtttcaac agcgagtacg aacgtatccc gagcttttac ggcaggttcg tttgaaaacg
     1021 ttgttccgcg ctgcaagccg ggagaagata gagaggcatt caaacaggtc gggaaaaacg
     1081 gcgaaagagg cgagagaatg agtgtggaac ggctggagga tgcgagaagg
//
LOCUS       DAOSWW010001013         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_122049_length_1130_cov_1.825116, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001013 DAOSWW010000000
VERSION     DAOSWW010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122049_length_1130_cov_1.825116"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..>1130)
                     /locus_tag="PK442_15050"
     CDS             complement(189..>1130)
                     /locus_tag="PK442_15050"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOO89023.1"
                     /translation="GHLILLPFNGQQIWDAVFDGRSLKMYTKFEEPEDTRTLVDTYGM
                     FMMHCGALRMGCPAPEDDHPLHGELTTAPYQEASVLFGEDDNGRFLALSGVYHYRKGF
                     GDFYDALPSVKLYERSSLFDISMEIRNRSEYPMDLMYMCHLNFLAGDDSRILQTAPWE
                     ADSMVLRTSIPAHVKPTPQFLDFLARLKQAPKETAVIRASDVYDPEIVFFIGGVRADT
                     EGRAHFMQRHPGGVSDYVSYDTVEFSHHVRWILKNKNQKVLGILPSTCEPEGYAAEKK
                     KGNVRSLAGNASVVFRIRAGALDSAQGDVYEKKLQNS"
BASE COUNT          253 a          321 c          301 g          255 t
ORIGIN      
        1 agtctggcca gcgccaccgc ctggttcgcg ccctttcctc caaatccgat gtcgaaatga
       61 ggagcctcga cggtctctcc aagtttgggc atcctgtcga taagggtaac caagtcgatc
      121 atattactcc cgatgacagc cgctttcggt cgtgcgttca ttccttttcc ccctgtctta
      181 tgtgtatctc aggaattctg tagctttttt tcgtacacat cgccttgagc gctgtccaga
      241 gcccccgccc ggatacggaa aacaaccgat gcgtttcccg ccagagaacg gacattcccc
      301 ttttttttct ccgcggcata cccctccggt tcgcacgtcg aagggaggat accaaggacc
      361 ttctgatttt tgttcttcag tatccaccgg acatgatgag aaaattccac agtgtcgtaa
      421 gaaacgtagt ccgagacacc tcccgggtga cgttgcatga aatgagctct cccctccgta
      481 tccgcccgaa cgccgccgat aaagaagacg atttccggat cgtacacatc cgaagcgcga
      541 atgacggcgg tttccttggg agcctgcttc agacgggcaa gaaaatccaa aaactgtgga
      601 gtgggcttca catgcgcggg gatgctggtc cgcaagacca tggagtccgc ctcccaggga
      661 gcggtctgca aaattctgga gtcgtcgcct gcaagaaagt tcagatggca catatacatc
      721 aggtccatag gatactcgct ccggtttcgt atctccatgg agatatcgaa gagcgacgag
      781 cgctcgtaca gtttcaccga ggggagcgca tcgtaaaaat ctccgaatcc cttccggtag
      841 tgatacactc cggaaagcgc gagaaatcgc ccattgtcgt cctcgccgaa gagaacggag
      901 gcctcctgat acggagcggt ggtcagctcc ccgtgcaggg ggtgatcgtc ttcgggagcg
      961 gggcagccca tgcgaagggc tccgcagtgc atcatgaaca tcccgtaagt atccacaagc
     1021 gtgcgagtat cctcgggttc ctcaaatttc gtgtacattt tgagagatct gccgtcgaag
     1081 acggcgtccc atatctgctg gccgttgaag ggaaggagaa tcagatggcc
//
LOCUS       DAOSWW010001014         1129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_122226_length_1129_cov_2.033520, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001014 DAOSWW010000000
VERSION     DAOSWW010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122226_length_1129_cov_2.033520"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PK442_15055"
     CDS             <1..739
                     /locus_tag="PK442_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO89024.1"
                     /translation="SFLTGLPVAEELRSRVPNTVILLGTSLLLSAALGTWLGLKAALR
                     RGSLLERCVLWSGALSFSFPSFFVQLLLLMFFASMIPLFPLRGTLSVPPPAGGPALLF
                     DYLRHMTLPVFSLVLLGFGGWAIYVRNLTVKILGEDFILLARARGLPKKNILRGHVFR
                     TMLPPLLTIFLLSLPGVVSGAVITETVFSLHGVGRFLLEAVTGHDYPAAGAAFFLLSL
                     LTVTCNLLSDLLYGLTDPRVRLERGRR"
     gene            739..>1129
                     /locus_tag="PK442_15060"
     CDS             739..>1129
                     /locus_tag="PK442_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO89025.1"
                     /translation="MRHLFTRWSFWSLLLLVLTALFGGRLFDAPPGREVAAAFSKPLW
                     IDRDLPPTKQLRLTADSPSLRFDWQYAPPSGLSVRIDPSVGQTGRFVEWRKPGAPEPL
                     RLAPLRGGFEADGRDIAFKRSLGLPPFG"
BASE COUNT          129 a          408 c          339 g          253 t
ORIGIN      
        1 ctcctttctg actggcctgc cggtggccga ggagctgcgc tcccgagtac cgaacacggt
       61 gattctgctg ggaacgagcc tcctgctctc cgccgcgctg gggacctggc tcggcttgaa
      121 agcggcgctg cggcgaggca gcctcctcga acgctgcgtc ctctggagcg gcgcgctttc
      181 cttctccttt ccctcgtttt tcgttcagct gctgctcctg atgttcttcg cctcgatgat
      241 cccgctcttt cctcttcgcg ggacgctctc cgttcctccg cccgcgggag gtcccgcgct
      301 gctgttcgac tacctgcggc acatgacgct gcccgtcttc tcgctcgtgc tgctcgggtt
      361 cggcgggtgg gcgatctacg tgcgcaacct gacggtgaag atcctcggcg aggacttcat
      421 tctcctcgcg agggcgcgcg gcctgccgaa gaaaaacatc cttcgcggac acgtcttcag
      481 aacgatgctc ccgcccctgc tcaccatctt cctgctctcg cttccgggcg tcgtctcggg
      541 cgccgtgatc acggagactg tcttctcgct tcacggcgtg ggacgcttcc tgctggaggc
      601 ggtaaccggg cacgactacc ccgccgccgg cgcggccttc tttctgcttt ccctgctcac
      661 cgtgacgtgc aacctgctct ccgacctgct ctacggcctc accgacccca gggtgcgcct
      721 ggaaaggggg cgccgctgat gcgacacctt ttcactcgct ggagcttctg gtctctgctg
      781 cttctggttt tgacggctct tttcggagga cgcctcttcg acgcgccccc cggacgggag
      841 gtggcggcgg ccttctcgaa gcctctctgg atcgaccgcg acctgccgcc gacgaagcag
      901 ctccggctca cggcggattc cccctcgctt cgtttcgact ggcagtacgc tcctccctcg
      961 gggctctccg tgcggatcga tccctcagta gggcagaccg ggcgttttgt cgaatggcgg
     1021 aagccgggcg ctccggagcc gttgcgtctc gctcctctca gggggggctt cgaggcggac
     1081 ggccgcgaca tcgccttcaa gcgctccctt ggacttcccc cattcggcg
//
LOCUS       DAOSWW010001015         1127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_122588_length_1127_cov_2.482276, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001015 DAOSWW010000000
VERSION     DAOSWW010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122588_length_1127_cov_2.482276"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1127)
                     /locus_tag="PK442_15065"
                     /pseudo
     CDS             complement(26..1127)
                     /locus_tag="PK442_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
BASE COUNT          206 a          346 c          306 g          269 t
ORIGIN      
        1 cgggacgtca gggcgatcct ggctccagta ttttgagacc tccgagagcc tttacctttt
       61 cgcgttccgc gcggcactgc ccacggtatt tctcaagaag cgcagaatac tgttccgaac
      121 tctccgcggc ttctccgttc tcccggcgaa gggcttccct tgccaggaac tcgggattcc
      181 cgccgagaag aatgtccgta ccgcgtcccg ccatattagt ggcgacagtc acagcgccga
      241 gatgccccgc ttgagcgaca atctgcgctt cccgctcgtg atgcttggca ttgagcacct
      301 gatgcgggat cttacgcgcc ttgagcagct tgctcagacg ctccgagctc tctatggatg
      361 tggtgccaac aaggacaggg cacccttcct tatatgattc ttctatctcg tccgcagcgg
      421 cggaaaactt ttccaaaacg gttctgtaga tgacgtccgg gcagtcctcg cgaatcatct
      481 ccacattcgt cgggacaaca ataacttcaa ggccgtagat ctccttgaac tcctcagctt
      541 cggtcgcggc ggtacccgtc attccggcga gcttgcggta catgcggaag tagttctgaa
      601 gcgttatggt ggccagggtc tggctctccc tgccgacttt tacaccctcc ttggcttcga
      661 tggcctgatg cagtccgtcg gagtaacgcc ggccgaccat caaacggcct gtgaattcgt
      721 cgacgatgat gatttctccg tccttcacga cgtagtggac atccttctgg aagagaatgc
      781 gcgctttcag ggcctgcacg atccggtgcg ccagatcgga gtgcgcggca tcggaaaaga
      841 ggtccggcag cttgaggagc tcctcgcatc tggcgatacc ggcttcggtc atcgcaacgt
      901 tccgctcttt ctcgtccttc tcgaagtcgc gcccctcttt caactgacgg gctatccgat
      961 ctgatgtggt gtacatctcc acgttgtcgt ccgatggacc ggagatgatg agcggcgttc
     1021 gcgcttcgtc gatcaggata gagtcgacct cgtccacgat acagtacgcg tgtcccctct
     1081 gcacaagatg cgactcgttc accgccatgt tgtcgcgcag atagtcg
//
LOCUS       DAOSWW010001016         1126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_122738_length_1126_cov_2.941176, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001016 DAOSWW010000000
VERSION     DAOSWW010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122738_length_1126_cov_2.941176"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1126
                     /locus_tag="PK442_15070"
     CDS             <1..>1126
                     /locus_tag="PK442_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOO89026.1"
                     /translation="GILSTFALLLMAPALAKFSLRFGPPEYFVLAIMGLSSIVTLTKG
                     SMVKGLLSGFLGLTIATVGMDPLSGFMRFTFGQMDLFDGVPFMPALIGLFSISQILDL
                     TAETRITENIDDATLSSIKRSKLPKGLAGTICRGGVIGTIVGMLPGAGATISSFISYN
                     FAKQSAKDPETFGKGNPKGVAAAESANNGCVGGSLIPLLTLGIPGNSVAAALMGGLMI
                     QGLIPGPELFSRYGTLTYGFILSLFIANIVFLVLGLYLAPYFARVTSTPNALLIPGIA
                     ILSVIGSYAINNNMFDVWLMIAFGIAGYFLEKGGFSTGALVLGLILGPIAELGFGQSL
                     IMSQGSPMIFFDRPLCILLWVITLLLMIPAFSSSLKKTKNR"
BASE COUNT          202 a          383 c          297 g          244 t
ORIGIN      
        1 ggcatactct ccaccttcgc cctcctgctc atggcgcccg ccctcgccaa gttctccctt
       61 cgcttcggac ctcccgagta cttcgtcctc gccatcatgg ggctctccag catcgtcacg
      121 ctcacgaagg gaagcatggt caagggactg ctctccgggt tcctgggtct caccatcgcg
      181 acagtgggca tggacccgct ctccggattc atgcgcttta ccttcggtca gatggacctt
      241 tttgacggcg ttccctttat gcctgcgctc atcgggctct tctcaatcag tcagattctc
      301 gatctgacag cggagacccg catcaccgaa aatatcgacg acgcgacgct ctccagcatc
      361 aaacggagca agctgccgaa aggactggcg gggacaatct gcagaggcgg cgtcatcggc
      421 accatcgtcg gcatgctgcc cggcgccggg gcgaccatct cctcgttcat ctcgtacaac
      481 ttcgcgaaac agagcgcgaa ggacccggag acgttcggaa aggggaaccc caagggagtg
      541 gccgccgcgg aaagcgcgaa caacggctgc gtgggaggct cgctcatccc gctgctcacc
      601 ctcggcattc cgggaaacag cgtcgccgcc gccctgatgg gggggctgat gatccagggg
      661 ctcatcccgg gccccgagct tttttcacgc tacggcacgc tgacctacgg attcatcctc
      721 tccctcttca tcgcaaatat cgtcttcctg gtcctggggc tctacctggc gccctacttc
      781 gcccgcgtca cgtcgacgcc gaacgcgctg ctcattccgg gaatcgccat tctctctgtc
      841 atcggcagct acgccatcaa caacaacatg ttcgacgtct ggctgatgat cgcgttcggc
      901 atcgccgggt acttccttga aaaaggaggc ttttctaccg gcgcgctcgt cctggggctc
      961 atcctcggcc ccatcgcgga gctcggattc ggccagtcgc tcatcatgtc gcaaggttct
     1021 ccgatgatct tctttgaccg cccgctctgt atccttctct gggtgatcac cctgcttctc
     1081 atgatccccg ctttttcgag cagtctgaaa aagacaaaaa acagga
//
LOCUS       DAOSWW010001017         1123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_123319_length_1123_cov_2.587079, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001017 DAOSWW010000000
VERSION     DAOSWW010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123319_length_1123_cov_2.587079"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PK442_15075"
     CDS             <1..795
                     /locus_tag="PK442_15075"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOO89027.1"
                     /translation="DFIGAVLVNGAVREGFEIKTSGNIEIRGVVEGAHLLSKGNILIS
                     GGVRGMNKAFLDASGDVTAGFIDQAKVRSGKDISVTNAVLHSDLAAHGKISVLGGNKA
                     QIAGGKIQAGLEVVCLTLGSEMGTKTEVVVGVLPEITERKKEILALLAETEEKAAKVE
                     ANLGFLKKAEAIEQLDENKRALMISLTKAKFQMQNQINTARDELKIIEEQMESSKTRG
                     CVRVKGHCYPGATVSIRGMTYIVREKQQFCAFVYDEGEIRVKPYDY"
     gene            835..>1123
                     /locus_tag="PK442_15080"
     CDS             835..>1123
                     /locus_tag="PK442_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89028.1"
                     /translation="MVTPVNLQLSHWNLEHTAQQTRDPSAMAAQAGRQGETIAAAEHR
                     DTSVQAADSSAEEERLGRKKRREEEEQRGGRQGKGQKGKHTEAAVEDESPSS"
BASE COUNT          309 a          259 c          380 g          175 t
ORIGIN      
        1 gacttcatag gcgcggtgct cgtcaacggc gcggtccggg aaggtttcga gatcaagacc
       61 tcgggaaaca tagagattcg cggcgtggtc gaaggagcgc acctcctgag caaggggaat
      121 atcctgatca gcggcggagt cagaggaatg aacaaagcct ttctggatgc ctcgggcgac
      181 gtgaccgccg ggttcatcga ccaggcaaag gtgcggagcg gaaaggatat ttcggtgaca
      241 aacgcagtgc tgcacagcga tctggccgct cacggaaaga tcagcgtcct cggcggaaac
      301 aaggcgcaga tcgccggcgg gaagatacag gcgggacttg aggttgtctg ccttacgctc
      361 gggagcgaaa tgggaaccaa gaccgaggtg gttgtgggcg tactgccgga gatcaccgaa
      421 cggaagaagg agatcctcgc gctcttggcc gagacggaag agaaggcggc caaggtggag
      481 gcgaatttgg gatttttaaa gaaggctgaa gcgatcgaac agcttgacga gaataagcgg
      541 gcgcttatga tcagtctcac aaaggcgaag ttccagatgc agaaccagat caatacagct
      601 cgcgatgagc tgaagataat agaggaacag atggagagca gcaagacgag agggtgtgtg
      661 cgggtcaaag gacactgcta cccgggggct acggtctcca tcagaggaat gacgtatatc
      721 gtccgggaaa agcagcagtt ctgcgctttt gtctacgatg agggagagat cagagtaaaa
      781 ccctacgact actaaaccaa gcgccgacga cttgcaggaa ggagggggag atctatggtt
      841 acgccggtga atctgcaact gtcccactgg aatttggaac acacggccca gcagacgcgg
      901 gacccttccg cgatggctgc gcaggcagga agacaggggg agacgatcgc ggcggccgag
      961 catagggata cttccgtgca ggcggccgac tcctccgccg aggaagaacg gctcggaagg
     1021 aaaaaacgac gtgaagaaga ggaacaaagg ggcggacggc aaggaaaagg acaaaaggga
     1081 aaacacaccg aggcggcagt tgaagatgag tctccttctt ctt
//
LOCUS       DAOSWW010001018         1122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_123595_length_1122_cov_1.912840, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001018 DAOSWW010000000
VERSION     DAOSWW010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123595_length_1122_cov_1.912840"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..>1122
                     /locus_tag="PK442_15085"
     CDS             375..>1122
                     /locus_tag="PK442_15085"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOO89029.1"
                     /translation="MTALCRSASLRLFQPDSSTVELADRIGCSLLTAAVLESRNLAAA
                     DERAPSLPDASGVESARESLFLDAKIPFEDLMEELSLGENAAEAADRWRSLKTLGRVL
                     VYGDYDADGVSSAALALELCRGKAERARFFIPHRHEQGYGLHESVLDQLLDIGWDTLV
                     VVDCGTKDGEILEKVARSGVNVFVFDHHLPEEGAPLHSAVVNPHTCAPRSAPGENEAG
                     RALCATGVLWAWAWKHSLLPRKRLAAMTDLV"
BASE COUNT          220 a          309 c          363 g          230 t
ORIGIN      
        1 attcctccgc tctggcccgg gctcgagtcc gatggtgaga agtagattct ttcgggagtt
       61 tacttctcca tcctcaccga ctacaataaa caaaaggata gaaatgaagc ggagctgcac
      121 ggttcatcgt gagcaggctc cgcagttttc tgcggagcgc cgctgagagc tccgcccgga
      181 atacacgcac ggaaaacaga cggccggccg gaagatggag taatgcacgt cttccgttac
      241 gatatatggt gaaatgagga tgtatttatg gagtattttc agacagcaga aagatgtgcg
      301 aatgacatcc gggcccggta cggcgtacgg tgtatccaaa aaaacagtgc ggaacggaaa
      361 gggaacggag tcctgtgacg gcgctctgtc gttccgcttc gcttcgtctc tttcagcccg
      421 actcctcgac cgtggagctt gcggacagaa tcggctgttc gttgcttaca gcggctgtcc
      481 ttgagagccg caacctggcc gctgccgatg agcgggcccc atctcttccg gacgcttccg
      541 gagtggagag tgcccgtgag agtctgtttc tcgatgcaaa gataccgttc gaagacctca
      601 tggaggagct gtctcttggg gagaacgccg ccgaagcggc agatcgctgg cggagcctca
      661 aaacgctcgg cagggtgctt gtgtacggcg attacgacgc ggacggggtg tcgtcggctg
      721 cgcttgccct ggagctctgc cgtgggaagg ccgagcgcgc ccggtttttt attcctcatc
      781 gccatgagca ggggtacgga cttcacgaat cggtgcttga tcagctcctc gatatcggct
      841 gggatacgct cgtcgtggtg gactgcggca cgaaggacgg ggagatcctg gagaaggtcg
      901 cgcgttcagg cgtcaatgtc ttcgtcttcg accaccacct tcccgaagag ggggcgccgc
      961 ttcacagcgc ggtggtgaat ccgcatacgt gcgcgccccg gtcggcgccg ggggagaacg
     1021 aggccggcag agcgctgtgc gctaccggag tgctctgggc atgggcatgg aagcactcgc
     1081 tccttccccg aaaacgtctc gcggcgatga cggatctcgt cg
//
LOCUS       DAOSWW010001019         1121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_123679_length_1121_cov_3.172608, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001019 DAOSWW010000000
VERSION     DAOSWW010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123679_length_1121_cov_3.172608"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1121)
                     /gene="pyk"
                     /locus_tag="PK442_15090"
     CDS             complement(<1..>1121)
                     /gene="pyk"
                     /locus_tag="PK442_15090"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOO89030.1"
                     /translation="VACRVIVGGLLADTKGINIPGAELSLPALSEKDIEDIKWGVQHE
                     MEYIAVSFVKNRRDIMEVRRIMEEFGEPMKVIAKIETRQAVQNIEEIVEVVDGMMIAR
                     GDLGVEIPTEDVPLQQKRIIDICRAKGKAVIVATQMLDSMIRNPRPTRAEASDVANAV
                     LDGADAVMLSGETAKGAYPLRSVETMKRIVARAESEIELWERPLHAVSTTMGVPDAVS
                     SAAVLIARQMKASAIVSMTKSGSTAQMVSKHRPPCTILGATPSQRTWRELSLYWGVLP
                     LMKEEMTDQNTAVDSVLGSCVASGHIREGDLVVVTAGVPLGTAGTTNLVQVHIAGKIL
                     LKGLSLLKREARGAVCVARSASEAREKMTKGAVLVARNT"
BASE COUNT          187 a          375 c          320 g          239 t
ORIGIN      
        1 cggtattcct ggcgacgagc acggcgcctt tggtcatctt ctccctggcc tcggaggcgc
       61 tcctcgctac gcagacggcc cccctggcct ctctcttgag cagggagagc cctttcagca
      121 gtattttccc cgctatatgc acctggacaa ggttcgtggt ccccgccgtg cccagcggaa
      181 cgccggcggt gacgaccacg aggtctccct ccctgatgtg cccgcttgcc acgcaggagc
      241 cgagcacgga gtccaccgca gtgttctggt cggtcatctc ctctttcatc agaggaagaa
      301 ctccccagta cagcgaaagc tcccgccatg tacgctggga aggcgtcgcg ccgaggattg
      361 tgcacggagg gcggtgcttg ctgaccatct gcgcggtgct gccgctcttc gtcatcgaga
      421 cgatggcgga ggccttcatc tgccgcgcaa tgagcactgc ggcgctgctc accgcgtccg
      481 gcacgcccat cgttgtggaa acggcgtgca gcgggcgctc ccaaagctcg atttcactct
      541 cggctctggc gacgatacgc ttcatcgttt ccactgagcg gagaggatac gctccttttg
      601 ccgtctcgcc ggagagcatg acggcgtcgg ctccgtcaag aaccgcattc gccacgtcgc
      661 tggcctcggc cctggtaggc cgggggttgc gtatcatcga gtcgagcatc tgcgtcgcga
      721 caataaccgc cttccccttg gcgcggcaga tatcgatgat ccgcttctgc tgcaaaggca
      781 cgtcttccgt gggaatttcc accccgagat cgccgcgggc gatcatcatg ccgtccacga
      841 cctcgacgat ctcctcgata ttctgcaccg cctgacgggt ctctatcttg gcgatgacct
      901 tcataggctc gccgaactcc tccatgatgc gccggacttc catgatatcc cgccggtttt
      961 tgacgaagga gacggcgata tactccatct cgtgctgtac tccccacttg atgtcctcga
     1021 tatccttctc ggagagcgcg ggaagagaga gttccgctcc cggaatattg attcccttgg
     1081 tatcggcgag cagtcctccc acaatgaccc ggcaggcgac g
//
LOCUS       DAOSWW010001020         1120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_123861_length_1120_cov_3.242254, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001020 DAOSWW010000000
VERSION     DAOSWW010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123861_length_1120_cov_3.242254"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PK442_15095"
     CDS             <1..441
                     /locus_tag="PK442_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89031.1"
                     /translation="PKAEDEIPRRESFLLPSEEKRRRASLVMENSGTLEEFRALAIKQ
                     ADLFKRASAIVRGSVLFQKNEDARRYAEYLHKERLGTEFLCTPAETFDRNEKGWFFTF
                     FTLEQWFSLLSQPHCMDSARGPYIERIRRIPWSRSLALLEELRP"
     gene            438..>1120
                     /locus_tag="PK442_15100"
     CDS             438..>1120
                     /locus_tag="PK442_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOO89032.1"
                     /translation="MKVITSHMGNDFDSLASMVAAQKLYPDAKLCLSGSAGRTVREFL
                     KKNASHWTVLTPRKIKFDEITMLIVVDARSRSRIGPFASLLGKKSIAVHVYDHHPPSN
                     DDIDGEFLAIEPVGACTTLMVEILLEKRIPIAPHEATLFATGIYEDTGGLTFSGVTAR
                     DFAAVSRLKEIGGDIASIPSFIEMTFSAPERRILDALVENAWVRFINGAKAVFSAVSA
                     PSYVDGLSL"
BASE COUNT          251 a          308 c          298 g          263 t
ORIGIN      
        1 ccgaaggcag aagacgagat cccgcggcgt gaatcttttc ttctgccttc ggaggaaaaa
       61 cggagacgcg cttctctggt catggagaac tctggaacgc tcgaggagtt tcgcgctctt
      121 gcgatcaaac aggctgattt gtttaaacgg gcgagcgcga tagtgagagg ctcagttctc
      181 tttcaaaaaa acgaagacgc ccgaaggtat gcagaatatc ttcacaagga acgtctggga
      241 accgagtttc tttgcactcc cgccgagaca ttcgaccgga atgaaaaagg gtggtttttt
      301 accttcttta cgcttgaaca gtggttttcg cttctttcgc agccgcactg catggattca
      361 gcccggggac cgtacatcga acggatacgc cgtataccat ggtcccgaag tcttgcatta
      421 ttggaggaac ttcgtccgtg aaggtcataa cgagccacat gggcaacgat ttcgactccc
      481 tcgcgagcat ggtggcggcg cagaagctct atcctgacgc aaagctgtgt ctttcaggct
      541 ccgcgggccg gactgtgcgg gagtttctga agaagaacgc ttcacactgg accgtgctca
      601 cgccgagaaa gatcaagttc gacgagatca ctatgctcat tgtcgtggac gccaggtcac
      661 gatcccgaat cggtcctttt gcctcgctgc tcgggaaaaa aagtattgca gtccacgtct
      721 atgaccatca ccccccatca aacgacgaca tcgacggcga atttctcgcc attgaacccg
      781 tcggcgcgtg cacaacgctc atggtggaga ttcttttgga aaaacgcatt ccgatcgctc
      841 ctcatgaagc cacgctcttt gctacgggga tctacgagga tacgggcgga ttgaccttca
      901 gcggcgtcac agcgagggat tttgccgccg tttcccgcct gaaggagata ggaggcgaca
      961 tcgcttccat tccctccttc atagaaatga cgttttccgc cccggaacgg cgcattctcg
     1021 acgcccttgt tgaaaatgcc tgggtacgct tcattaacgg agcgaaggct gttttctcag
     1081 ccgtttccgc tccatcgtac gtggacggac tctcgctctt
//
LOCUS       DAOSWW010001021         1118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_124257_length_1118_cov_3.259643, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001021 DAOSWW010000000
VERSION     DAOSWW010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124257_length_1118_cov_3.259643"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PK442_15105"
     CDS             <1..780
                     /locus_tag="PK442_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO89033.1"
                     /translation="YVPAESLVHRLDPRCKILAVLVVLTGIFLARHILVFAAWGGLLF
                     SLAVLSHLPFRLVLRSAKPVLILVFFTAFIHVFFTDGTPLLEIGFITITREGVSGAVY
                     MGLRLLFLVLFASFLTLTTKPMELSDGLERLFSPFVRFGFPAHELAMMMTIALRFIPT
                     LLDETDRIMKAQLARGAAFDRGNLWKRLKAFLPVLIPLFVIVFQRAEDLATAMEARCY
                     RGGEGRTRMHPLVWRTGDTLGALCVLAIVAGLIAGDRSFLS"
     gene            777..>1118
                     /locus_tag="PK442_15110"
     CDS             777..>1118
                     /locus_tag="PK442_15110"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HOO89034.1"
                     /translation="MKYAAVVGYRGTSFSGWQRQTSGLGVQEKIEDSLRIISGREISV
                     TAAGRTDAGVHSRGQVISFELEKVWRPDKLLLAMNFHLPPDVSVMKIAHAPQSFDARR
                     SALWREYRYLIW"
BASE COUNT          174 a          365 c          321 g          258 t
ORIGIN      
        1 tacgtgcccg ccgaatctct tgtgcatcga ctcgacccgc gctgcaagat tctcgccgtg
       61 ttggtggtcc tcacagggat cttccttgcg cggcacattc ttgtttttgc ggcatgggga
      121 gggcttctct tttctcttgc cgtgctctcg caccttccgt tccgccttgt ccttcgctcg
      181 gcgaagcccg tgctcatact cgtcttcttt acggcgttca ttcatgtttt tttcaccgac
      241 ggaacgccgc tgctggaaat cggctttata accattaccc gcgagggagt ctccggcgcc
      301 gtctacatgg ggctgcggct tctcttcctc gttcttttcg cctcgttttt aacgctcacg
      361 accaagccca tggagctctc cgacgggctt gaacgtctct tctccccgtt cgtccggttc
      421 ggcttccctg cgcatgaact ggccatgatg atgacgatcg cgctccgctt catccccacc
      481 cttctcgacg aaaccgaccg catcatgaag gcgcagctcg cgagaggagc ggccttcgac
      541 cgcgggaacc tctggaaacg cctcaaggcg ttcctccccg tactcatccc tctcttcgtc
      601 atcgtcttcc agagggcgga ggatcttgcc accgccatgg aggcacggtg ttacagaggg
      661 ggggaggggc gcaccaggat gcatccgctt gtctggagga ccggagatac gctcggcgcg
      721 ctgtgcgtcc tggcgattgt cgcgggactg atcgccggag accggagctt tctttcgtga
      781 agtacgccgc ggtcgtcgga taccggggga catccttctc cgggtggcag cgccagacca
      841 gcggtctcgg cgttcaggag aagatcgagg acagcctccg gatcatctcc gggcgagaga
      901 tctccgtcac ggcggcaggc cgcaccgacg cgggcgtcca ttcccggggg caggtcattt
      961 cttttgaact tgaaaaggtc tggcggccgg acaaactcct gctcgccatg aactttcacc
     1021 tcccccccga cgtgagcgtc atgaagatcg cccatgcgcc gcaaagcttc gacgcccgcc
     1081 gttccgcgct ctggagggag taccggtacc tgatatgg
//
LOCUS       DAOSWW010001022         1118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_124325_length_1118_cov_2.209784, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001022 DAOSWW010000000
VERSION     DAOSWW010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124325_length_1118_cov_2.209784"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PK442_15115"
     CDS             <1..496
                     /locus_tag="PK442_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89035.1"
                     /translation="EQKGIYSPFWSADGASVYGQDLDTLYRIDAESGAVVERRPLASV
                     TGGKAMVSSALRFSLSPDGTMWLFDAEVEDTGKLMKHGEPLNSAIFLRTPADGKTRRL
                     TPETICAMHPSWLPGGREFLFAGYGPKEAKRKGSPFAIFRRGFDGTKTTLLIKDGDSP
                     SAGL"
     gene            complement(570..>1118)
                     /locus_tag="PK442_15120"
     CDS             complement(570..>1118)
                     /locus_tag="PK442_15120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOO89036.1"
                     /translation="SGGARRMTDILSEKKRSETMSSIRSADTKPEWILRCGLHRMGFR
                     YRLRDKKLPGTPDLVFPKYRTVVFVHGCFWHRHEGCRKSTFPKSRQAFWAEKFAGNVE
                     RDARTRAELERAGWRVIVVWECELEGDTEATLERTARLLRAPSDAEQAIPPPRRELLD
                     IASEKVRLRVASYKAGKKPAEE"
BASE COUNT          198 a          352 c          371 g          197 t
ORIGIN      
        1 ggagcaaaag gggatctact cccccttctg gtctgcggac ggagcgtccg tctatgggca
       61 ggaccttgac acgctctacc ggatcgacgc cgaaagcggc gcggtcgtcg agcggcgtcc
      121 gctcgcttcg gtcaccggcg gcaaggcgat ggtgtcgagc gccctccgat tctccctctc
      181 gccggacggg acgatgtggc tcttcgacgc cgaggtggag gacaccggaa agctgatgaa
      241 acacggcgag ccgctgaact cggcgatctt cctgcgcacc ccggcggacg ggaagacgcg
      301 gcggctgacg ccggagacga tctgcgcgat gcacccctcg tggctccccg gagggaggga
      361 gttcctcttc gcagggtacg ggccgaagga ggcgaagcgg aagggctcgc ccttcgcgat
      421 cttccggcgt ggattcgacg ggacgaagac aacgctcttg ataaaagatg gagacagtcc
      481 atcggcgggg ctgtaaaaaa gaccgcacaa tgcaaccggg gttccgtgct gtttaaccgg
      541 acaggagaac ccggaagcaa ccgcagatat cactcctccg ccggtttttt cccggccttg
      601 tacgaagcga cgcggaggcg gactttttcg gaagcgatat cgagcagctc tctgcgcggc
      661 ggtggaatcg cctgttccgc gtcggacggc gcgcgaagca atcgggccgt gcgttcgagc
      721 gtcgcctccg tgtccccctc gagttcgcac tcccagacga cgatgacgcg ccaccccgca
      781 cgttcgagtt ccgcccgcgt ccgcgcgtcg cgctcgacgt tgccggcgaa cttctccgcc
      841 cagaatgcct gcctgctctt gggaaaggtc gacttccggc atccttcgtg cctgtgccag
      901 aagcagccgt gcacgaagac gaccgtccgg tacttcggga agacgaggtc gggtgttccg
      961 ggaagttttt tgtcgcgcag acggtagcgg aaccccatcc ggtggagtcc gcaacggagt
     1021 atccactcag gcttcgtatc cgcgctccgg atggaggaca tcgtctcgga gcgcttcttc
     1081 tcggagagta tgtcggtcat gcggcgagcg ccgccgga
//
LOCUS       DAOSWW010001023         1117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_124412_length_1117_cov_4.133710, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001023 DAOSWW010000000
VERSION     DAOSWW010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124412_length_1117_cov_4.133710"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PK442_15125"
     CDS             complement(<1..244)
                     /locus_tag="PK442_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO89037.1"
                     /translation="MSAPVLGIDLGTRYALSAVPVKGKGVLVVPSRWGGGRTPSVTAL
                     TQSGWLAGEDAARSEMKNPSTTWWDMKRKLGSPWRAR"
     gene            complement(241..>1117)
                     /locus_tag="PK442_15130"
     CDS             complement(241..>1117)
                     /locus_tag="PK442_15130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO89038.1"
                     /translation="IHFCSYCAIPYARGFPVSRSPDDVLKEIRSIVSSDCREVVLTGV
                     HLGLYGEYGEISLAELVRKVSLVEGLRRLRFGSIEPLGIDDGLLETLAETHAFQPHLH
                     IPLQSGSDRVLSLMNRGYSTAEYRDIVQRVRAFLGDDVHVSTDLLVGFPGEDERAFAE
                     TLAFLEECRFGKVHVFPFSPREGTKAFSLPGRVPPQELSSRSKRALETGEKLLLEYSA
                     RWIGRTVPVLLESVSSPESSDGAGWVFSGLSPSFLRVTGGGESGADPEFSAGDEVLVR
                     ITGSRDDSLKGRLIQ"
BASE COUNT          275 a          329 c          291 g          222 t
ORIGIN      
        1 atcgtgcccg ccatggagaa ccaagttttc gtttcatgtc ccaccaggtt gtggacgggt
       61 ttttcatttc acttcgcgcc gcatcctccc cggcgagcca ccccgattgc gtcaaggccg
      121 ttaccgaagg cgttcgtcct cctccccagc ggctcggtac caccaggact ccctttccct
      181 taacagggac tgcggagagc gcatatcgcg ttccaagatc aataccaagg acaggggcgc
      241 tcattgaatc aggcgacctt tcaaagaatc atcacgtgag ccggtaatcc tgacaaggac
      301 ctcatctcct gctgaaaatt caggatcggc tccggattcc cctcctcccg tcacgcggag
      361 aaaggacgga gaaagcccgc taaataccca tcccgcgccg tcggaggatt cgggggacga
      421 gacgctctcc aagagcaccg gaacagttcg acctatccag cgggcactgt attcgagcaa
      481 caatttttct cctgtttcaa gagccctctt gcttcttgag gagagctctt gaggaggcac
      541 tcttccaggg agagagaagg ctttcgtccc ttcacggggg gagaacggga agacatgaac
      601 cttcccgaag cggcactctt caaggaaagc aagagtctct gcgaatgcgc gttcgtcctc
      661 cccggggaag ccgacgagca aatcggtact gacgtggaca tcatccccga gaaaagcacg
      721 aactctttgc actatatcac gatactccgc cgtcgaatac cctctgttca taagagagag
      781 tacccggtcg cttccgctct gaaggggaat atggagatgc ggttggaaag cgtgagtttc
      841 tgcaagcgtt tcaagcaggc cgtcgtcgat tcccagaggt tcgatcgaac cgaagcgaag
      901 acggcgaagc ccctcgacca aagatacttt tctgacaagc tccgccaaag atatctcacc
      961 gtactcgccg tacagcccca gatgcacccc ggtgagcacg acctcacggc agtcggaaga
     1021 aactatggaa cgaatctcct tcagtacatc gtccggagag cgactgacgg gaaatcctcg
     1081 agcataggga attgcacagt aagaacagaa atgaatg
//
LOCUS       DAOSWW010001024         1117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_124421_length_1117_cov_3.506591, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001024 DAOSWW010000000
VERSION     DAOSWW010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124421_length_1117_cov_3.506591"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PK442_15135"
     CDS             <1..244
                     /locus_tag="PK442_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOO89039.1"
                     /translation="LLRKEAKREAVERGVSLLLVDGPPGVGCPVISSLTGADFAVLVT
                     EPSRSALSDLRRVISLCTMLSVPFGIAVNRWDLSKE"
     gene            241..417
                     /locus_tag="PK442_15140"
     CDS             241..417
                     /locus_tag="PK442_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89040.1"
                     /translation="MTSAIRAACDEEGWSFLGAVPFSEKTARAVNEGRIPVKEIGDPL
                     RELWRAILEKGREH"
     gene            419..754
                     /locus_tag="PK442_15145"
     CDS             419..754
                     /locus_tag="PK442_15145"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO89041.1"
                     /translation="MAKIGIPSLGTDEASAVADRFARAPFFMIADEKGAILESFENTA
                     GSEAHGAASKALMALSQKEVSILLVPRLGPNAIGFAKQAGMSAFCAEGLTVKDALAKY
                     AQGSLEKLL"
     gene            812..>1117
                     /locus_tag="PK442_15150"
     CDS             812..>1117
                     /locus_tag="PK442_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOO89042.1"
                     /translation="MTLPPKSDKKQIKKIYAVGSGKGGVGKSSVTSLLAIAAARQGKR
                     VGILDADITGPSIPTFFGLHTFPFGNAEGIEPPASRSGIRVISMNLLLDDPAKPVIWR
                     "
BASE COUNT          235 a          304 c          355 g          223 t
ORIGIN      
        1 ccttctgcgc aaggaggcga agcgtgaagc cgttgaaaga ggtgtttcgc tgcttctcgt
       61 ggacggacct cccggcgtgg gatgtcctgt tatctcttcg ttgacgggag cggactttgc
      121 ggtgctggtg accgagcctt cgcgctcggc gctttcggat ttgcgaagag tgatctccct
      181 ctgcacgatg ctctcggttc ccttcggaat tgcggtgaac cggtgggatc tttcaaaaga
      241 atgaccagcg caatccgcgc cgcctgcgac gaagaggggt ggtccttcct cggcgccgtg
      301 cctttttcgg agaagacggc gagagccgtc aacgagggac gcatcccggt gaaggagatt
      361 ggcgacccgc tgcgcgaact gtggcgggct atactcgaga aaggacgtga acattgatat
      421 ggcaaaaata ggaatcccct ctttgggaac tgatgaggca agcgctgtgg cggaccgttt
      481 cgcccgcgct ccgtttttta tgatcgccga tgaaaagggc gcgattttgg aatccttcga
      541 gaacacggcc ggaagcgagg cccatggggc cgcttccaag gcgctgatgg cgctttcgca
      601 gaaggaggtc tccatactgc tggttccgcg tctgggaccg aacgcgatcg gctttgccaa
      661 acaggccggg atgagcgcgt tctgcgccga aggattgact gtgaaagacg cgctcgcgaa
      721 gtatgcgcag ggttccctcg agaaattact gtaaatcagg agtacaatag actgtagcat
      781 ccataatacg acaaaggggc gaaccttttt tatgacactg ccaccgaaaa gcgacaagaa
      841 acagatcaag aaaatctacg ccgtcggaag cggcaagggc ggagtgggaa agagctccgt
      901 gacgtcgctc ctggcgatcg cagccgcccg tcaggggaag agggtgggca tattggacgc
      961 cgacatcacg gggccgtcca tcccgacgtt cttcggcctg cacaccttcc cgttcggcaa
     1021 cgccgagggg atcgagcctc cggcatcgcg ttccggaatc cgcgttatct cgatgaacct
     1081 gctgctcgac gacccggcaa aaccggtgat ctggcgg
//
LOCUS       DAOSWW010001025         1117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_124459_length_1117_cov_2.710923, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001025 DAOSWW010000000
VERSION     DAOSWW010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124459_length_1117_cov_2.710923"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1117
                     /locus_tag="PK442_15155"
     CDS             <1..>1117
                     /locus_tag="PK442_15155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOO89043.1"
                     /translation="LTVFMKMFNGLNSFVLLAVPLFILAANLMNHGQITRKLVNFCIA
                     LVGNIRGGLAHANILVSMIFAGVSGSSQADTAGVGKMLIPAMIESGYDKENSVGVTAA
                     SSTIGVIIPPSIPMVIYSGLTNASVAALFLGGMIPGILVGVTMMMIVYLIGHRRNFPK
                     YEKAKYSDVWKLFKESSPALLTPVIIVGGITTGWYTPTEAAAFASVYALLISLFIYKT
                     LKLRDLPGILKETLKLSSLSLFALATASALGELLGYYKASEGVAAFFAANVGNPRIFL
                     LIVIAFFLFVGTFMDAIPAMILFIPVILPTAVGMGISPVHLGVVVVMTLAVGLVTPPY
                     GLCLLIAGSIADLSIERSLKGVLPYISALMIILLLVAM"
BASE COUNT          188 a          375 c          300 g          254 t
ORIGIN      
        1 tctcaccgtc ttcatgaaga tgttcaacgg tctcaactcg tttgtgttgc tcgccgtacc
       61 gctcttcatc ctggcggcca acctgatgaa ccacggacag atcacccgga agctggtaaa
      121 cttctgcatc gcgcttgtgg ggaacatccg cggcggcctc gcgcacgcga atatcctcgt
      181 gtccatgatc ttcgccggag tctccggctc ctcccaggcg gacacggcgg gcgtcgggaa
      241 gatgctgatt cccgcgatga tcgagagcgg gtacgacaaa gagaactccg tcggcgtcac
      301 cgcggcgtcc tcgaccatcg gcgtcatcat cccgccgagc atcccgatgg tgatctactc
      361 cggcctcacc aacgcctctg tcgcggcgct ctttctcgga gggatgatcc ccggcatcct
      421 cgtcggcgtc accatgatga tgatcgtcta ccttatcggc caccggagga acttcccgaa
      481 gtacgagaag gcgaagtact ccgacgtctg gaagctcttc aaggaatcgt caccggcact
      541 gctgacccct gtcatcatcg tcggcggcat caccacagga tggtacacgc ccacggaggc
      601 ggcggccttc gcctccgtct atgcccttct catcagcctt tttatataca aaacgctgaa
      661 actgcgcgat ctacccggaa tcctgaagga gacgctgaag ctcagctcgc tctcgctctt
      721 cgcgcttgcc acggccagcg ctctcggcga actgcttggc tactacaagg cttccgaagg
      781 cgtcgcggct ttcttcgcgg caaacgtcgg caatcctcgt atcttcctcc tgatcgtgat
      841 cgccttcttc ctcttcgtcg gcacctttat ggacgcgatc ccggcgatga tcctcttcat
      901 cccggtcatc ctgccgacgg ccgtcggcat ggggatttct ccggtccacc tcggcgtcgt
      961 ggtcgtcatg acgctcgcgg tcggcctggt gacgccgccg tacggactct gtttgctcat
     1021 cgcgggctcg attgcggatc tctccataga acgctctctg aagggcgtcc ttccctacat
     1081 ctccgcgctg atgatcatcc tgttgctggt ggcgatg
//
LOCUS       DAOSWW010001026         1117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_124493_length_1117_cov_2.141243, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001026 DAOSWW010000000
VERSION     DAOSWW010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124493_length_1117_cov_2.141243"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..>1117
                     /gene="hflK"
                     /locus_tag="PK442_15160"
     CDS             136..>1117
                     /gene="hflK"
                     /locus_tag="PK442_15160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOO89044.1"
                     /translation="MRNIFDEIEELYEKTRRKERNFDGEEYGGGGGNEKPRRRFSFSF
                     GFLKLLLPVLLLLIVAWQGFYIVPAGSRGVVFRLGKISHVANQGMNFKLPVVDLVTIV
                     NTEQIQRFEYGYRTVNPGPPPTYSDKPEESKMLTGDNKIVEIDWVLQYQAGTPEEYVV
                     NLPGDQWAREKMIRDIAESTLREVIASRALDDVLTTEKEASQTEAKMLIQEKLNALKT
                     GVFIVAVQFQDVNPPKAVQAAFSEINTARSERERLILEADKYSNEIISKAQGESDRIL
                     NEAEAYKFRRISLAEGEAARIRSLNAAYKENPDLVLNNLWLESMVKVWPKL"
BASE COUNT          267 a          315 c          347 g          188 t
ORIGIN      
        1 ccccggacgc cttggaccgc tccccttctg gcgaggtaag gagaccttcc cggagacgat
       61 ggcggctctc tacgaatcag cgacgtgcag ggcggaagag atctatcgca cacttgaaga
      121 aaacggaggt atcagatgag aaatattttc gatgaaatcg aggagcttta cgaaaagacg
      181 agacgcaagg aaagaaactt cgacggcgag gagtacgggg gaggcggggg gaacgaaaaa
      241 ccccggcgcc gtttctcctt cagcttcggc ttcctgaaac ttctgctccc cgtactgctt
      301 ctgcttatcg tcgcttggca ggggttctat atcgtcccgg cagggtcgcg gggcgtcgtc
      361 ttccgccttg gaaagatctc ccacgtggcg aaccagggaa tgaacttcaa acttcccgtc
      421 gtcgacctgg tcaccatagt gaacacggaa cagatccagc gcttcgagta cggctaccgg
      481 acggtgaacc ccggacctcc cccgacgtac tcggacaagc ccgaggagtc gaagatgctc
      541 accggcgaca acaagatcgt cgagatcgac tgggtgctcc agtaccaggc gggaacgccg
      601 gaggagtacg tggtgaacct tccgggagac cagtgggcgc gtgaaaagat gatccgcgac
      661 atcgcggagt ccaccctgcg ggaagtcatc gcctccagag cgctcgacga cgtgctcacc
      721 accgagaagg aggcctcgca gacggaggcg aagatgctca tccaggagaa actgaatgcc
      781 ctgaagacgg gggtcttcat cgtcgcggtg cagtttcagg acgtgaaccc gccgaaagct
      841 gtccaggcag cgttcagcga gatcaacacg gcgcgctcgg agcgtgagcg gctgatcctg
      901 gaagcggaca aatactcgaa cgaaatcatc tcgaaggcgc agggcgaatc cgaccgcatt
      961 ctgaacgaag cggaggcata caagttccgc agaatctcgc tcgcggaggg cgaggcggcg
     1021 cgcatccgtt cgctgaacgc agcgtacaag gaaaatcccg atctggtgct caacaatctc
     1081 tggcttgaga gcatggtcaa ggtctggccg aagctga
//
LOCUS       DAOSWW010001027         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_124737_length_1116_cov_1.834119, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001027 DAOSWW010000000
VERSION     DAOSWW010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124737_length_1116_cov_1.834119"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1116)
                     /gene="istA"
                     /locus_tag="PK442_15165"
     CDS             complement(<1..>1116)
                     /gene="istA"
                     /locus_tag="PK442_15165"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOO89045.1"
                     /translation="QFCLCYQRYARGANATMRMVRKPGESAEVDWAGQKGFWLDPETG
                     ERNEASIFVGVLSSSRYAYVEAFPSQNQESWLNAHVNMFRYFGGVPRILIPDNLKTGV
                     TKPDRYEPVVNRLYRELAEHYGTAVIPARVGKPRDKANAEGTVGAITTWILAALRERN
                     FFSLRELNEGIREKLDRFNAKPFQKKEGSRLDAFLAEEKDLLLPLPKNPYELSSWKKV
                     TADKSYHVLVEKMLYSVPYEYIGHEMEVRLTRNVVEVFFEGQRICYHARMFGRIGQCV
                     TVPSHMPPSHREHRQWEGSRFIEWAADVGPHTEIVMRGLLSAHKIEQQSYKSCQGLVR
                     LGDVHSFERLEAACGKALLYTPRPGYRNVKTILTTGQ"
BASE COUNT          170 a          346 c          342 g          258 t
ORIGIN      
        1 cctgtccggt ggtcagtatt gtcttgacgt tcctgtaccc cggacggggt gtgtagagaa
       61 gcgctttccc gcaggcggcc tccagtcttt cgaaggagtg gacgtctccg agcctgacga
      121 gcccctgaca gcttttgtag ctctgctgct cgatcttgtg ggcggagaga agtccgcgca
      181 tgacgatctc ggtgtgcggg ccgacgtcgg cggcccattc gatgaaacgg cttccctccc
      241 actgcctgtg ctccctgtgc gagggcggca tgtgcgacgg gaccgtgacg cactgtccga
      301 tacggccgaa catccgggcg tggtagcata tcctctggcc ttcgaagaag acctccacga
      361 cgttcctcgt caggcgtacc tccatttcgt gtccgatgta ctcgtacggg acgctgtaga
      421 gcatcttctc gaccagtacg tggtacgact tgtccgccgt gaccttcttc cacgacgaga
      481 gttcgtacgg attcttcggc agcggcagga gaaggtcttt ctcctcggcg agaaaggcgt
      541 cgagcctgct cccctccttc ttctggaagg gtttggcgtt gaagcggtcg agcttctcgc
      601 gtatcccctc gttcagctcc cgcaggctga agaagtttct ttcgcggagc gcggccagta
      661 tccatgtggt gatcgctccg acggttcctt cggcgttcgc cttgtccctg ggctttccaa
      721 cccgtgcggg gatgaccgcc gtgccgtagt gctccgccag ctccctgtaa agccgattga
      781 ctacgggttc gtatctgtcc ggcttggtga ctccggtctt gaggttgtcg ggtatcagga
      841 tgcggggcac gccgccgaag taccggaaca tgttcacgtg cgcgttcagc cagctctcct
      901 gattctgcga gggaaaggcc tcgacgtagg cgtaccggct gctggagagg acgcctacga
      961 agatggacgc ttcgttgcgc tcccccgtct ccggatcgag ccagaacccc ttctgtcccg
     1021 cccagtcgac ttccgcgctc tcgccgggtt tgcggaccat tcgcatcgtc gcgttcgcgc
     1081 cacgtgcgta tctctggtag cagagacaga actggg
//
LOCUS       DAOSWW010001028         1112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_125303_length_1112_cov_3.674551, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001028 DAOSWW010000000
VERSION     DAOSWW010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125303_length_1112_cov_3.674551"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1112
                     /locus_tag="PK442_15170"
     CDS             <1..>1112
                     /locus_tag="PK442_15170"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HOO89046.1"
                     /translation="AAGQSTALPLSIDSDSALVLESGLGAVTGIPLINSFTAREDSLF
                     PGLGLAKRHGAAAVVLPIDENGLPESEEARKRVILKILESADSLGFPREGLIVDGLTL
                     AVGADHAAPAVTLSALTFLREQGIASVLGVSNVSHGMPARSLLNRTFLAMAVAAGLDA
                     AIMNPLDRSMMETLAAAETLAGRDAGGRRFLGAAASFSAPSDAPRPAAHAAGQSARDE
                     TLAVDPRFLPLALSILRGDAQQAEANAQELLGSGTTPLDVVNKGVIPALEEVGKRYDT
                     GAFFLPQLISSAETAQRVCELAMELVQRSGGIASKGRVLLATVEGDLHDLGKNVVGTV
                     LKSHGFTVVDLGKDVPSARILEEAEKGAFDFVGLSA"
BASE COUNT          188 a          376 c          350 g          198 t
ORIGIN      
        1 ccgccgcagg ccagtccacc gcgttgccgc tctccatcga cagcgacagc gctttggtgc
       61 ttgaatcagg gctcggggcc gttaccggta ttccactgat caactccttc accgccagag
      121 aggactccct cttccccggc ttggggctgg caaagcgcca cggcgctgcg gcggtcgtgc
      181 ttccgatcga cgagaacggt cttcccgaaa gcgaagaggc ccgtaaaaga gtcatcctta
      241 agattctcga aagcgcggac tctctgggat ttccgcgtga aggcctcatc gtcgacggac
      301 tgacgcttgc cgtcggcgcg gatcacgccg ctccggcggt cacgctctcc gccctgacct
      361 tcctcagaga gcaggggatc gcctcggttc tgggggtaag caacgtctcg cacggcatgc
      421 ccgcccgttc gctcctcaac aggacgttcc tcgcgatggc cgtcgccgcg gggctggacg
      481 ccgccattat gaacccgctc gaccgctcca tgatggagac cctcgccgca gcggagacgc
      541 ttgcaggacg cgatgcggga gggcgtcgct tcctcggcgc ggccgcttcg ttctcggcgc
      601 cttccgacgc gccccgtcct gcggcacacg ccgcgggtca gtccgcaaga gacgaaacgc
      661 tcgccgtcga cccccggttc ctgccgctcg ccctttcgat acttcgcggc gacgcgcagc
      721 aggcggaggc gaatgcgcag gaactcctcg gctcaggaac aaccccgctc gacgtggtga
      781 acaaaggggt cattcccgcg cttgaagagg tcgggaagcg ctacgacacc ggcgccttct
      841 tccttcccca gcttatctcg tcggcggaga ccgcgcagcg cgtctgcgaa ctcgctatgg
      901 aacttgtgca gcgatcgggt gggatcgcgt cgaaggggag agtgctcctc gccactgttg
      961 aaggagacct gcacgacctg gggaagaacg tcgtcggcac ggttctcaag agccacggtt
     1021 tcacagtggt cgacctgggg aaggacgtcc cctccgcacg tattctcgaa gaggccgaaa
     1081 agggagcgtt cgatttcgta gggctctccg cg
//
LOCUS       DAOSWW010001029         1112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_125306_length_1112_cov_3.580889, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001029 DAOSWW010000000
VERSION     DAOSWW010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125306_length_1112_cov_3.580889"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..584)
                     /locus_tag="PK442_15175"
     CDS             complement(150..584)
                     /locus_tag="PK442_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOO89047.1"
                     /translation="MHKFPVLKDGVNGPMLEEVRAKVNTWLREFFDEDDLVRVEDLVS
                     FSFGSATIQVTVLPWHSGDVLVKVFSYLAENVDKNKVAAEFLRLNADLPLGGFSLVFD
                     NTVMLSCSLPGAKLDKSELLGAVQTVAVYADQYDEILKDLYR"
     gene            complement(577..>1112)
                     /locus_tag="PK442_15180"
     CDS             complement(577..>1112)
                     /locus_tag="PK442_15180"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOO89048.1"
                     /translation="AKAMANERNLRKQQENALSQAKQWEEKAAMALKAGNADLAKQAL
                     SKKIIADGQAKQYDAMVAQASSVTSQLRSQLDTLKTRLDEARMKQATLVARAQTAKTQ
                     KEFSTVMGSNVGQGAFAKFEKMEKKIEGMEAEAQAFSELSGDAPADDPFKDMEKDMQL
                     EAEMAKLMEKMGGGAGA"
BASE COUNT          204 a          383 c          274 g          251 t
ORIGIN      
        1 gccttcataa acttcagctc ggtcacaagc ccttcagact tcttcttctg gatgaacgag
       61 agatacgcgc cccctgcgat catcagaccg ccgataccaa gagccacgaa attcatgcaa
      121 cctcctcctt tcctcccctt cagggaaaac tacctgtaca gatccttcag gatttcgtcg
      181 tactggtccg cgtagacggc gaccgtctgc accgctccca gcagttcgct cttgtccaac
      241 ttcgctccgg gcagagaaca ggagagcatc acggtgttgt cgaaaacaag cgagaagccg
      301 ccgagcggca ggtcggcatt cagtcgcagg aattctgccg caaccttgtt cttgtcgacg
      361 ttctctgcca ggtaggaaaa gaccttcacc agaacatccc cgctatgcca cggaagcacc
      421 gtcacctgga tcgtcgcgct gccaaaggag aaggagacga ggtcctccac ccgcacaaga
      481 tcgtcctcgt cgaagaactc ccgcagccag gtattcacct tcgcccggac ctcctccagc
      541 attggtccgt tcacgccgtc cttgagtacg ggaaacttat gcacccgctc caccccccat
      601 cttctccatc agtttggcca tctccgcctc aagctgcata tccttctcca tgtccttgaa
      661 cgggtcgtcc gcaggggcgt cgccggagag ttccgagaac gcctgcgcct ccgcctccat
      721 cccctcgatc ttcttctcca ttttctcgaa cttcgcgaag gcgccctggc cgacgttact
      781 ccccatgacc gtggaaaact ccttctgcgt cttcgccgtc tgagcgcggg cgactaaagt
      841 cgcctgcttc atccttgctt cgtcgagccg cgtcttgagc gtgtcgagct gtgaacgaag
      901 ctgggacgtg acgctggaag cctgcgccac cattgcgtcg tactgctttg cctgtccgtc
      961 ggcaatgatc ttcttggaga gcgcctgctt cgcaagatcg gcgttccccg ccttgagggc
     1021 catcgcagcc ttctcctccc actgtttcgc ctgagagagc gcgttctcct gctgcttgcg
     1081 gaggttgcgc tcgttggcca tcgccttcgc ca
//
LOCUS       DAOSWW010001030         1111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_125508_length_1111_cov_3.703598, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001030 DAOSWW010000000
VERSION     DAOSWW010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125508_length_1111_cov_3.703598"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PK442_15185"
     CDS             <1..784
                     /locus_tag="PK442_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOO89049.1"
                     /translation="GSVIEKGEKVCFTFEEFAFRSKHFFERMKQLLSLLDREMGVPAD
                     VEFAFHPTEDLVEIVQARPLWISEQTSTATIPPLENRNVVLRADRMVTHGVKENIPYL
                     VYIDHHVYAQETRFHDVARAVGSVNDLLKGSRFVLVAPGRVGSSNPLLGVPVQYNEIT
                     RCCCIVEVGFPKEGYMPELSFGTHFFTDLEIDGILYMPVYEGAKNNVFDEHFFDSSPY
                     TLGGHAGVRIYSGSFSVYTDGDKNLGVVVADRVDEPESGWAE"
     gene            complement(850..>1111)
                     /locus_tag="PK442_15190"
     CDS             complement(850..>1111)
                     /locus_tag="PK442_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOO89050.1"
                     /translation="VAKGGKPVVTIEVPRLDERRLGALIQLYEHVTALTGYVLDVNPF
                     DQPGVEQGKNYTYGLMGRPNFSAQALEVTELAEKLSLMEVTL"
BASE COUNT          235 a          301 c          313 g          262 t
ORIGIN      
        1 cggctcggtc atcgaaaagg gagaaaaggt ctgtttcact tttgaagaat tcgcattccg
       61 ttcaaaacac tttttcgaga ggatgaaaca gttgctctct cttcttgaca gggagatggg
      121 tgttcctgcc gacgtcgagt tcgcgttcca cccgaccgag gatcttgtgg agatcgtcca
      181 ggcccgcccc ctctggatca gcgaacagac gtctacggcg accatacctc ctcttgaaaa
      241 ccgcaatgtc gtgctgcgcg cggatcgaat ggttacgcac ggcgtaaagg aaaacatccc
      301 ttatcttgtc tatatcgacc atcacgtcta cgcgcaggag acgcgttttc acgacgtcgc
      361 gagggcggtt ggttctgtga acgatttgtt gaaaggctcg agattcgtcc ttgtggcgcc
      421 gggacgcgtc ggttcgagca acccgctgct gggagttccc gtccagtaca acgagattac
      481 gcgctgctgc tgcatcgtag aggtagggtt tccaaaggag gggtacatgc ccgaactctc
      541 gttcggcacg cacttcttca ccgacctgga gatagacggt attctgtaca tgccggtgta
      601 cgagggcgca aagaacaacg tcttcgacga acacttcttc gattcctctc cctatactct
      661 tggcggacac gcaggagtgc gtatctactc gggctccttt tcggtgtaca ccgacggaga
      721 caaaaatttg ggcgtcgttg tggcggacag ggtggacgaa cccgagtcag ggtgggcgga
      781 atagttttcg atactgcgaa ggccgcgcgg agagttgctc ctgcgcggcc ttcttttttt
      841 cagaaaaact cagagggtga cttccataag agaaagtttc tcggcaagct ccgtcacttc
      901 aagagcttgg gcgctgaaat taggccgccc catcagtccg taggtgtaat tcttcccctg
      961 ctccaccccg ggctggtcga acgggttcac atcaagaaca tatccggtga gcgctgtaac
     1021 atgttcgtag agttgaatca gggcgccgag ccgccgttca tccaagcgcg ggacctctat
     1081 ggtcacgacc ggtttccctc ctttggcgac a
//
LOCUS       DAOSWW010001031         1111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_125674_length_1111_cov_1.671402, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001031 DAOSWW010000000
VERSION     DAOSWW010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125674_length_1111_cov_1.671402"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PK442_15195"
     CDS             complement(<1..633)
                     /locus_tag="PK442_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HOO89051.1"
                     /translation="MQLSLVEYLSDPRTLYTAWRSVWKKAGAPGCDGVRTQDFFCNEA
                     ANIRNLSSELRSGKYQPSPLRETFIPKSTPGKWRRLSIPTVKDRIVQQAANQILQTFC
                     DDAFAPCSFAYRPGRGIREAVALLRKGIQSGLVWAVRGDIRGCFDEIDRDILSALLRR
                     LLPDQEFLALINKVIRSPVVSKGFVRNSSKGIPQGSPLSPFLSNLYLDQFD"
     gene            complement(665..949)
                     /gene="cas2"
                     /locus_tag="PK442_15200"
     CDS             complement(665..949)
                     /gene="cas2"
                     /locus_tag="PK442_15200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOO89052.1"
                     /translation="MSRMHCVISYDIVNNKRRRKLAGLLEGYAARVQYSVFEGWLANG
                     QLKTLVHESARFIKICEGDSFRIYRICASCYPKTVAIGGFIPDWESAIIV"
BASE COUNT          266 a          320 c          279 g          246 t
ORIGIN      
        1 gtcgaactga tccagataca gattcgaaag aaaaggagag agaggagaac cttgaggaat
       61 tcctttcgat gaattacgga cgaacccctt actgaccacc ggtgagcgaa tcaccttgtt
      121 gatcagagca agaaactcct gatcgggaag aagcctccgg agcagtgcag aaagaatatc
      181 tctgtcgatc tcatcgaagc accctctgat gtctccccga actgcccaga caagaccgga
      241 ttgaatgcct ttccgcagca gagccaccgc ctcacggatt cctctgcccg gacgataggc
      301 gaagctgcac ggagcgaacg catcgtcgca gaacgtctgc agtatttgat ttgccgcttg
      361 ctgaactatc ctgtccttaa ccgtgggaat agacagccgt ctccacttgc cgggcgtgct
      421 cttcggaatg aacgtctcgc gcagaggaga aggctggtat ttccccgaac gcagttcgga
      481 gctcaaatta cggatattgg ccgcttcatt gcagaaaaag tcctgcgtcc ggacaccgtc
      541 gcacccgggt gctcccgcct ttttccacac cgaacgccat gcggtgtaca aagtccgggg
      601 atccgaaaga tactccacca gagaaagctg cattcaacat gcctcctttt gcgttctccg
      661 tttttcagac gatgatggcg ctctcccagt cggggatgaa gccccctatg gcaaccgtct
      721 tgggataaca cgacgcacag attctataga tgcggaaaga atctccttcg catatcttga
      781 tgaaacgagc gctctcatgg acaagagttt tcagctgtcc gttcgcaagc catccttcaa
      841 aaacgctgta ttgaacacgt gcggcgtatc cctccagcaa acctgccagc ttgcggcgcc
      901 ttttgttgtt cactatgtcg tacgaaataa cgcagtgcat tctgctcatc cggatacctc
      961 ctcgcgctac ttcttcgcca cgaacggaat gtaatccgac tcatgccaaa tcgccctcgc
     1021 cagactctcc gcctgggagt ggatgcgacg cctgtaggga agcgaatgtt ccgtagtctc
     1081 cgatgagctg gacgaggcga gcctggcatt g
//
LOCUS       DAOSWW010001032         1107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_126318_length_1107_cov_4.742395, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001032 DAOSWW010000000
VERSION     DAOSWW010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126318_length_1107_cov_4.742395"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..415)
                     /locus_tag="PK442_15205"
     CDS             complement(41..415)
                     /locus_tag="PK442_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOO89053.1"
                     /translation="MTAEHLLTGRRGEELAEAHLRAVGWTILERNVTFRGGELDIVAR
                     DGNELVVVEVRTRAPGWMQSGEESVGPRKIRRLVRTGMKYMDSRKWDGPWRIDVVAVT
                     LPENGDAHIERFEDITAGGNFV"
     gene            complement(412..690)
                     /locus_tag="PK442_15210"
     CDS             complement(412..690)
                     /locus_tag="PK442_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOO89054.1"
                     /translation="MSPPKKKRAQAAAVQYDQKNDDAPRIVASGEGYVAEKIVELARQ
                     ADVPVVEDAALVSALLVLELGEEIPGELYEAVAKILTFVYKLDKGEKL"
     gene            complement(687..>1107)
                     /locus_tag="PK442_15215"
     CDS             complement(687..>1107)
                     /locus_tag="PK442_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89055.1"
                     /translation="DDDIAAFLRGHGLLSSPSREGVDPSLLAGAFLPAGDDAQPLNAR
                     IATSAFKRGEKSFWSVSFEMEGDRLGLVAGDVETDGRSLSVVLKAEQEASFEELRRRR
                     HILRHELEEIPLSLQNVAVAHGRRMPRVPGRGLDITV"
BASE COUNT          188 a          387 c          313 g          219 t
ORIGIN      
        1 accgcttcga taccgcggag cgtgatcccc tggacgccgt tcatacgaag ttgcctcccg
       61 ccgtgatgtc ctcaaaccgc tcgatatgcg catctccgtt ctccggcagc gtcacggcca
      121 ccacgtcgat ccgccacgga ccgtcccatt tgcgggaatc catgtatttc atcccggtgc
      181 ggacaaggcg gcggatcttt ctcggcccca ccgactcctc gccgctctgc atccaaccgg
      241 gcgcgcgcgt gcgcacctcc acgacgacga gctcgttccc gtcgcgggcg acgatatcca
      301 actcgcctcc cctgaaggtg acgtttcgtt caagaatcgt ccatccgacg gcgcgcagat
      361 gcgcttcggc aagctcctcg ccgcggcggc ccgtcagaag gtgctccgcc gtcatagttt
      421 ttctccctta tcgagtttgt agacgaaggt gagtatcttg gcgacggcct cgtacagctc
      481 tcccgggatt tcctcgccca gttcaaggac gagcagcgcg ctcacaagcg cggcgtcctc
      541 gacgacggga acgtcggcct ggcgcgcgag ctccacgatc ttctccgcga cgtacccctc
      601 tccagaggcg acaatgcgcg gagcatcgtc gttcttctga tcgtactgga ctgcggccgc
      661 ctgggcgcgc ttttttttcg gggggctcat accgtgatgt ccagtccacg ccccggcacg
      721 cgaggcattc gccttccgtg ggccacggcg acgttctgca gggaaagcgg aatttcttcg
      781 agttcatgac gaagaatatg gcggcgacgg cgaagctcct cgaagctcgc ctcttgttcc
      841 gccttcagca ccacgctcaa ggatcgtcca tccgtctcca catctcccgc cacaagcccg
      901 agccgatccc cctccatttc aaaggagacg gaccagaacg atttttctcc tcttttaaag
      961 gcgcttgtgg caatccgggc gttcagcggc tgcgcgtcgt ctccggcggg gaggaatgcc
     1021 cccgcgagca gcgacggatc caccccttcg cgcgacgggc tggaaagaag accgtgcccg
     1081 cgcagaaagg cggcgatatc gtcgtcc
//
LOCUS       DAOSWW010001033         1106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_126547_length_1106_cov_3.020932, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001033 DAOSWW010000000
VERSION     DAOSWW010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126547_length_1106_cov_3.020932"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PK442_15220"
     CDS             complement(<1..492)
                     /locus_tag="PK442_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOO89056.1"
                     /translation="MIRHIRSAVEKGNLILGYVSGLGILLMGLILFYEVVMRYFFKAP
                     TIWAQETAVYIFMWTMLAGGAYTLQKGKHVRIDLIIEHLSIRTQKILEMITSFAGMFF
                     CAVVTWQAYEMIAASIGYEKVSATPLRVPMWIPQMALLLGFALLTFQFAIIIADRYAE
                     VFGK"
     gene            complement(598..>1106)
                     /gene="dctP"
                     /locus_tag="PK442_15225"
     CDS             complement(598..>1106)
                     /gene="dctP"
                     /locus_tag="PK442_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOO89057.1"
                     /translation="DFKGLTMRTFSSGDAETLRALGAAPTVMSSSEMYMALQRGTVEG
                     ATTGMPAAVSRKIHEVQKYMTMANYTTAQFVVQANLGWWEKLSQAEKDVILKAGLDAE
                     AWIRQAIEASENEAEKVIRDAGVEIHVLSAEERPLFIEATDPVRKAFAEKTGEIGKTL
                     IDMVMAID"
BASE COUNT          220 a          341 c          292 g          253 t
ORIGIN      
        1 cttgccgaag acttctgcgt accggtcggc gatgatgatc gcaaactgga atgtcaacag
       61 agcgaaaccg agcagcagcg ccatctgcgg aatccacatc ggcacccgca gcggcgtcgc
      121 cgatactttt tcatacccta tggacgcggc gatcatctcg tacgcctgcc acgtgaccac
      181 cgcgcagaag aacattccgg cgaagctggt gatcatttcg aggatcttct gcgtccgtat
      241 cgagaggtgc tcgataatga ggtcaatgcg cacgtgcttc cccttctgga gcgtgtaggc
      301 tcctccggcg agcatcgtcc acatgaagat gtacacagcg gtctcctggg cccagatggt
      361 gggcgccttg aagaaatagc gcataacaac ctcgtaaaac agaatcagcc ccataagaag
      421 aatcccaagg ccgctgacgt agcccaggat caggtttcct ttctccaccg cactgcgaat
      481 atgtcgaatc atctacgcat gcccctttat tcataattta tagtaaagag cgggaaagag
      541 agtccctttc ccgctctttc gagacaactc ctgtgaggac tctttctccc gaaaaaccta
      601 gtcgatcgcc ataaccatgt cgatcagggt tttgcctatc tctcccgttt tttcggcgaa
      661 ggccttgcgc acaggatccg tggcctcgat gaagagcggc ctctcctcgg cgctcagtac
      721 gtggatctcc actccggcgt cgcggatcac tttttcggct tcgttctcgg acgcctcgat
      781 ggcctggcga atccacgcct cggcgtcaag accggccttc aggatcacat ccttctcggc
      841 ctgggagagt ttctcccacc agccgaggtt cgcctgcacc acgaactgcg ccgtagtgta
      901 gttcgccatc gtcatgtact tctgcacttc gtggattttg cgggagacgg cggccggcat
      961 gccggtcgtt gcgccttcta cggtgcctct ctgcagcgcc atgtacatct cggacgaact
     1021 catgacggtg ggcgcggcgc ccagcgcgcg cagcgtttcc gcgtcgccgc tgctgaaggt
     1081 tctcattgta agccctttga agtctt
//
LOCUS       DAOSWW010001034         1106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_126561_length_1106_cov_2.856327, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001034 DAOSWW010000000
VERSION     DAOSWW010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126561_length_1106_cov_2.856327"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..386
                     /locus_tag="PK442_15230"
     CDS             54..386
                     /locus_tag="PK442_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOO89058.1"
                     /translation="MDRKKELLMEYRQRKREMGVFLFECAEGGRYIGRADDPKGAINK
                     NVFQLKMGSHPNKRLQKEWSDRGGAAFSVRVLDSLEYDEETKAECAEELAMLFDDWIQ
                     KMKDAEVV"
     gene            388..882
                     /locus_tag="PK442_15235"
     CDS             388..882
                     /locus_tag="PK442_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HOO89059.1"
                     /translation="MKIPTTLKHKPVVAVENYENVDGRQAYNSEAKGLSLGLAQWNDR
                     GRVDVSAKVWRHTGGKWSRQSEELPMHRVIDMAILVCSAKRYFSESYRRPEPYDKETP
                     RIDRIGLQGDAMSVEICTGNPMIDQDIELFQEALRRDDELMAERLHTLANLLIGMGYG
                     NCAR"
     gene            958..1106
                     /locus_tag="PK442_15240"
                     /pseudo
     CDS             958..1106
                     /locus_tag="PK442_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
BASE COUNT          266 a          283 c          366 g          191 t
ORIGIN      
        1 attagtaaac gagtaattta gtaaatgact cgttggattc gaggtgtcgt atcgtggaca
       61 ggaaaaaaga gctgctgatg gagtacaggc agcggaagcg ggagatgggc gtctttcttt
      121 tcgaatgcgc ggagggcgga cgctacatcg ggcgcgccga cgacccgaag ggcgcgatca
      181 acaaaaacgt ctttcagctg aagatggggt cgcacccgaa caagcgcctg cagaaggagt
      241 ggtcggaccg cggcggggcg gcgttctccg tccgcgtgct ggattcgttg gagtacgacg
      301 aggagacgaa agcggagtgc gccgaagagc ttgcgatgct cttcgacgac tggatacaga
      361 aaatgaagga tgcggaggtc gtataatatg aaaatcccga cgacattgaa acataaaccg
      421 gtggtcgcgg tggagaacta cgaaaacgtg gacggacgcc aggcgtacaa ttccgaggcc
      481 aaggggcttt cgctggggct ggcccagtgg aacgaccggg gcagggtgga cgtttcggcg
      541 aaggtctggc ggcatacggg ggggaagtgg tcgcgccagt ccgaggaact gccgatgcac
      601 agggtgatcg acatggccat cctcgtctgc tcggcgaagc ggtacttttc ggagagctac
      661 cgccgccccg aaccgtacga taaggaaacc ccccgtatcg acaggatcgg gcttcagggg
      721 gacgcgatga gcgtggaaat ttgcaccgga aacccgatga tcgaccagga catcgagctc
      781 ttccaagagg cgctccggcg cgacgacgaa ctgatggccg agcggctgca caccctcgcg
      841 aatctcctga tcgggatggg gtatggaaac tgcgcgcggt gagggcgaag gaatctcccg
      901 agctccgcat aaaggggcgc aacggaacga tgtgcaggaa aattcctgca cgcgcttcct
      961 tccgccgatg aaacccggtc gattctggac gggcacatta tcctgtcgcg caaactggcc
     1021 gcagcgaatc attatcccgc aatcgacatt ctggccagtg tcagccgggt catgaataca
     1081 attgttgatc agcagcacaa aaccgc
//
LOCUS       DAOSWW010001035         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_126901_length_1105_cov_1.789524, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001035 DAOSWW010000000
VERSION     DAOSWW010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126901_length_1105_cov_1.789524"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PK442_15245"
     CDS             complement(<1..969)
                     /locus_tag="PK442_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO89060.1"
                     /translation="MKKKEILRLLLSLCILAYSFTAAFAADNVRPEPLLIQAQGSFAV
                     GGTILAESGTFDPKDPMKPDGQTFHGDHAYVFHQVPVNPRAFPIVFLHGAGQFSKTWE
                     TTPDGREGFQNIFLRRGFATYLLDQPRRGGAGRSTLPMELKPAGDEQMWFSIFRLGNW
                     PNFHPGVQFPQDEESLNQFFRQMTPNTGPYDDRIISDAIVALFDKLGGGILVTHSQGG
                     GPGWHTVLKSTKVNAVISYEPGLFIFPEGEVPPPMPSSSPFGELQAAGVPMQEFMKFT
                     KIPIALYFGDNIPEAPSDDPGKDNWRTRLQMAKLWVEAVNKHGGDAT"
BASE COUNT          228 a          309 c          311 g          257 t
ORIGIN      
        1 cgttgcgtct ccgccgtgtt tattgacggc ctcaacccaa agcttcgcca tctgaaggcg
       61 agtccgccag ttgtcctttc cagggtcgtc cgaaggcgcc tcgggaatgt tgtctccgaa
      121 gtagagcgcg atcgggatct tcgtgaactt catgaactcc tgcatcggca cgcctgccgc
      181 ttgcaactcg ccgaacggac tggaactcgg catcggcgga ggaacttcgc cttcggggaa
      241 gatgaacaaa ccgggttcgt aggagatgac ggcgttgacc ttcgtgctct tcagtacggt
      301 gtgccatccc ggaccgcctc cctgagaatg tgtgaccagg atgcctccgc ccaatttgtc
      361 gaagagagcg acgatcgcat cggaaataat ccggtcgtcg tacggccccg tattcggcgt
      421 catttgacgg aagaactggt tcaggctttc ctcgtcttga ggaaattgaa caccggggtg
      481 aaagttcggc cagttcccga ggcggaatat cgaaaaccac atctgctcgt ctccggcggg
      541 tttcagctcc ataggcagcg tgcttctccc cgcgcctccg cggcgaggct gatccagaag
      601 gtaggtggcg aatccgcgcc gcaggaagat gttttgaaac ccctctcgcc cgtccggcgt
      661 cgtttcccag gttttcgaga actgccccgc tccgtgaaga aagacgatgg gaaacgcgcg
      721 cgggttgacc ggaacctgat ggaaaacata ggcatgatcc ccgtggaatg tctgcccgtc
      781 gggcttcatg gggtccttcg gatcgaacgt tccggactcc gcaaggatcg tgcctccgac
      841 tgcaaagctc ccctgcgctt gaataagaag cggttctggt ctgacgttgt cggcagcgaa
      901 agcggctgtg aaagagtacg ccaaaatgca caacgatagc aagagcctca aaatctcttt
      961 ctttttcata tcaatggtcc tccttatatg gaatatactc tttttctctc tatattgaat
     1021 aacagagtca agtggaatcg gcgttttcgt gcgtgcaaag gattgagcgt tcaccggttt
     1081 tcttgtccgg cgccgcgatg ctctt
//
LOCUS       DAOSWW010001036         1101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_127672_length_1101_cov_1.867113, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001036 DAOSWW010000000
VERSION     DAOSWW010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127672_length_1101_cov_1.867113"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..>1101
                     /locus_tag="PK442_15250"
     CDS             150..>1101
                     /locus_tag="PK442_15250"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOO89061.1"
                     /translation="MDTRGILTLFEKIYGSSGMAESFFAPGRVNLIGEHTDYNGGHVL
                     PCALTFGTYIAARKRSDGTVRCFSENYPESGVVRFELDEMARDPRHGWVNYPKGVVKA
                     FRENGLPIGSGFDMVVRGDMPSGAGLSSSASIEMATAVMLSSLFSLGLEDEAGRVRMA
                     LLGKEVENRFIGVNSGIMDQFAVALGRAGTALFLDCTTLERRYVPLELGKYEIVVMNT
                     NKSRALVESRYNERRSECDRALADLQKSGVTIRSLGDLSVEEFEQAADAIQDPVCRRR
                     ARHAVLENARVLTAVAALESGRIAEFGGLLNESHVSLQFDY"
BASE COUNT          213 a          276 c          365 g          247 t
ORIGIN      
        1 atcttcggcg ttatcaacta cgggctgact ttcatcggtg tgaatccatt ctggcagctt
       61 attgtcaagg ggctcataat tgtggcggcg gtctccttcg acatcagaaa atacatagcg
      121 aagaagtaac ggctgcgtac ggagtgctta tggatacacg cggcatcctg acgctttttg
      181 aaaagatcta cggttcttcc gggatggcgg agtcattctt tgcccccgga agggtgaatc
      241 ttattggaga acatacggac tacaacgggg gacatgtgct gccgtgcgct ctgaccttcg
      301 gtacgtatat tgcggccaga aaacggtccg acggcacggt gcgctgtttc tccgagaatt
      361 atccggaatc cggggttgtc cgtttcgagt tggatgagat ggctcgcgat ccccggcacg
      421 ggtgggtgaa ctacccgaaa ggggtcgtga aggcgtttcg agagaacggt ctccctatcg
      481 gctctggctt cgatatggtc gtccgggggg acatgccctc cggagcgggg ctttcctcgt
      541 cggcgtcgat cgagatggcg actgcggtga tgctctcctc tctcttctcc cttggactgg
      601 aggacgaggc ggggcgcgtc aggatggcgt tgctcggcaa agaggtcgag aacaggttca
      661 tcggcgtgaa cagcggcatc atggaccagt tcgccgttgc gctgggacgc gcgggaacgg
      721 cgctctttct ggactgcacg acgcttgaac ggcgctatgt tcccctcgaa ctcgggaagt
      781 atgagattgt ggtgatgaac acgaacaaga gcagggcgct cgtcgaatcg aggtataacg
      841 agcgaaggag cgagtgtgac cgcgcgcttg cggatctgca aaagagcggc gtcacaatcc
      901 ggagccttgg cgatctttcg gtcgaggagt ttgaacaggc ggcggatgcc atccaagacc
      961 cggtttgccg gcggcgtgcg cggcacgctg ttttggagaa cgcgcgggtg cttaccgctg
     1021 tcgctgcgct ggagtcggga agaattgccg agtttggggg tctgctcaac gaatcgcacg
     1081 tgtcattgca gttcgactac g
//
LOCUS       DAOSWW010001037         1100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_127860_length_1100_cov_2.118660, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001037 DAOSWW010000000
VERSION     DAOSWW010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127860_length_1100_cov_2.118660"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PK442_15255"
     CDS             complement(<1..205)
                     /locus_tag="PK442_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89062.1"
                     /translation="MNAPRKWTIVLLMVSLVLSVSFAASAASYTSAPLNPEFVSMQQQ
                     GNSVKALSSTGKPLGYRPFPLDLS"
     gene            complement(312..998)
                     /locus_tag="PK442_15260"
     CDS             complement(312..998)
                     /locus_tag="PK442_15260"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOO89063.1"
                     /translation="MRIPLLAFLLFTLMLSVTAATVVEGSDSPLVEGMVTSEGKGVFG
                     IEINIWDGQTNRKAVSDSEGRFSIAGLEPGTTFALRSTPRNHRSVRADGFRFPEKGNL
                     YLSMEYAETQAGQRHSIRVPSNPSTGYEWFLLQQGDTAVAAFRGNTMETEAKTEETPG
                     TTEWQPTNQGEWKSPQPSAPPRTGRGGWERWTFQTFGEGQSVIVLGYIRPWETGVSPA
                     RYHVFSLLVR"
BASE COUNT          251 a          308 c          283 g          258 t
ORIGIN      
        1 gcgagaggtc gagagggaaa ggccgatatc ctaaaggttt ccccgtggac gatagtgctt
       61 ttacgctatt gccctgctgc tgcatcgaaa caaactcagg gttcaagggc gccgatgtat
      121 aagacgctgc acttgccgcg aacgagactg aaagaacaag cgataccata agcaatacta
      181 ttgtccactt gcgcggtgcg ttcatcgttt catcacttcc ctttcttcaa atagaccccc
      241 ggtaatgggg ccgcgaaaca tcttattcgt tcttcgggtt ttgaaagatc aagctccaga
      301 cgatcagctt gctaccggac aagaagagag aaaacgtggt accgagcagg ggaaacccct
      361 gtttcccaag gccggatata ccccagaacg atcacgctct gcccctctcc gaaagtctgg
      421 aacgtccatc gttcccatcc gcctctgccg gttcttggcg gagcggatgg ttgcggagat
      481 ttccactcgc cctggttcgt cggctgccat tcagtcgtgc ccggcgtttc ttcggttttc
      541 gcttcggttt ccatcgtatt tccgcggaac gccgcaactg cggtgtctcc ctgctgcagg
      601 aggaaccact cgtagcctgt cgaagggttc gaaggaacgc ggattgaatg ccgctgtcct
      661 gcctgagtct cggcgtactc catgctcaaa tagagattcc ccttctcggg aaagcggaat
      721 ccgtccgcgc gaacggatcg atgattgcgc ggggtggagc gaagcgcaaa cgttgttccc
      781 ggctcgagtc cagctattga aaatctgcct tcgctgtcgg aaactgcttt cctgttggtc
      841 tgaccgtccc atatgttgat ttcgatgccg aaaacaccct tcccctcgct tgtcaccatt
      901 ccttcgacaa gcggagaatc ggatccctct acaacagtag ccgccgtaac agagagcatg
      961 agcgtgaaca aaaggaacgc taaaagaggt attcgcatgt ctcttcacca ccttttcgag
     1021 atgtgtcgcc tcgaagtgag cgaggtccag cagaagcgac aggaatcgga cacatacttc
     1081 tattcataaa aactgtattc
//
LOCUS       DAOSWW010001038         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_127958_length_1099_cov_3.825670, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001038 DAOSWW010000000
VERSION     DAOSWW010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127958_length_1099_cov_3.825670"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PK442_15265"
     CDS             complement(<1..710)
                     /locus_tag="PK442_15265"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOO89064.1"
                     /translation="MSTLSLHERRALVDWEEKEIPLSWQAWLLGLNRSGLYYVPAPLS
                     EEELLLRRRIDEINTLHPCYGSRRMRAVLSRGGLSVSRKRIQRLMRDMGILSIYPAPK
                     LSAGDESPKFPYLLRGVDIDHPNHTWGTDITYVRLRGGFMYLTVFLDWYSRYVVSWAF
                     SDSLSAGFVIEALEDAFKTARPKIINSDQGSQYTSRLYVERIRDESDGIAISMDGRGR
                     CMDNIFTERFWRSYKQEEV"
BASE COUNT          210 a          330 c          284 g          275 t
ORIGIN      
        1 acttcctcct gcttgtagct cctccagaat ctctcggtga agatgttgtc catgcaacgc
       61 ccccttccgt ccatgctgat ggcgatgccg tcgctttcgt cccgaatccg ttcgacgtac
      121 agtctgctcg tgtactgcga cccctgatcg ctgttgatga ttttcgggcg ggccgtcttg
      181 aaggcgtcct ccagcgcctc gatcacgaac cccgccgaca gcgagtccga aaatgcccac
      241 gagaccacgt agcgggagta ccagtccaga aacaccgtaa gatacatgaa gccgcccctc
      301 agccggacgt aggtgatgtc cgtcccccac gtgtggttcg gatgatcgat gtccactccg
      361 cgcagaagat aggggaactt cggactctcg tcgcccgcgc tcagtttggg agcggggtat
      421 atcgagagga ttcccatgtc tctcatgaga cgctgaatcc gtttgcgcga tacggacaat
      481 cccccccgcg acagcaccgc ccgcatcctc ctggagccgt agcacggatg caacgtgttg
      541 atctcgtcga tcctcctgcg gagcagcagt tcttcctcgg agagaggcgc ggggacgtag
      601 tacagcccgc ttctgttcag accgagcagc caggcctgcc aggagagagg gatctctttt
      661 tcctcccagt cgacgagcgc tctgcgttca tgaagagaaa gagtcgacac cgcatttttt
      721 tttgatccat tcgagctgca tggaaagcaa cccgacttgt ctgtaaaggt cgtttatcgt
      781 agaatccttc ccgagatcct cggcgctttt cctgcgggga tcggagaaca atcccggaag
      841 cccttccgtg gccgtctttt tccatctctg aagcaggttg ggatgtattt tgtgcttggc
      901 tgcgatctgg ctgagagttt cttcttcacg aagcatctcg agcacaactt ttgccttgaa
      961 ttcagcagaa aagttccgac gcgaactcat cttttacacc ttaacctccc cccgattttt
     1021 tgtccgattt tcggggtcca ttatagcttt ctgtccgggt ggtattcgat gccttccatg
     1081 cacgcgtcgc ctattttca
//
LOCUS       DAOSWW010001039         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_127977_length_1099_cov_3.114943, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001039 DAOSWW010000000
VERSION     DAOSWW010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127977_length_1099_cov_3.114943"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PK442_15270"
     CDS             <1..970
                     /locus_tag="PK442_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO89065.1"
                     /translation="SQKGAMELAYLIARQRKRIHSSKRERLAELAAILGVPKIQQPLF
                     NGVGIYHGGMLPKEKILMEAAFRERILDVVCGTNALALGVNLPAETAVFAQLVHYHSD
                     APISKNEFLQMAGRAGRKGLFDPGYVTWLAKSPFERRGFSTKEIFNELLEAAPEKANV
                     SLRPSFGRLLRKEVLPEVEAEFIARYSLPQQDEYLVGNSIRSGMKRIDRAMSKMVHHH
                     ERKKFRKTLASLWYDEMDIEENLEMAFLFHTEQSPSALLAAQVILPFERNYLQALLKV
                     KRFANRMPKGFAFRSMKELNNTVDEIDPTIYGFEEKIGEIERSLFS"
BASE COUNT          241 a          310 c          326 g          222 t
ORIGIN      
        1 ttcgcaaaag ggggcgatgg agctcgccta cctgatcgcc cgccagagga agcggattca
       61 ttcctccaag cgcgaacgcc tcgctgaact tgcggcgatt ctgggcgtgc cgaagattca
      121 gcagccgctc ttcaacgggg tcggcatcta ccacggcgga atgctgccaa aggaaaaaat
      181 ccttatggag gcggccttca gagagcgaat cctcgacgtg gtctgcggaa cgaacgcgct
      241 tgcgcttggc gtcaacctcc ctgccgaaac cgccgtattt gctcagcttg tccactacca
      301 ctcggacgcg ccgatctcaa agaacgagtt tcttcagatg gcgggacggg caggacggaa
      361 ggggctcttc gacccggggt atgtcacgtg gcttgcaaaa tcgccgttcg agcggcgggg
      421 gttttcaacg aaggagatct tcaatgagct gcttgaggcg gccccggaga aggcgaacgt
      481 ctcgctgcgc ccgtctttcg gcaggctgct ccgcaaagag gtcctccctg aagtggaagc
      541 agagttcatc gcacgctact ccctgccgca gcaggacgag tatctcgtcg ggaactcgat
      601 ccgctccgga atgaagcgta tcgacagggc aatgtcgaag atggtccacc accacgagcg
      661 gaagaagttt cgaaagacgc ttgcctcgct ctggtacgat gagatggaca ttgaggaaaa
      721 cctggagatg gcgtttcttt tccacacgga gcagtcgccc agcgccttgc tggccgcgca
      781 ggtgatcctt ccctttgaaa ggaactacct ccaggcgctg ctcaaggtga aacgatttgc
      841 gaacaggatg ccgaaagggt ttgccttccg gtcgatgaaa gagttaaaca atacggttga
      901 cgaaatcgat ccgacgatct acgggttcga ggagaagata ggagagatcg aacgatcgct
      961 cttctcctga attttgagtg caagcgtctt taccctgctt tttcgctgta cgcaacgccc
     1021 ttccctacgt cgtcgagcgc ccggcggaca aagaggtcct gctgcaatcc caggtcgacg
     1081 atggttcctt ccggagcgc
//
LOCUS       DAOSWW010001040         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_128161_length_1098_cov_4.215724, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001040 DAOSWW010000000
VERSION     DAOSWW010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128161_length_1098_cov_4.215724"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK442_15275"
     CDS             complement(<1..637)
                     /locus_tag="PK442_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89066.1"
                     /translation="MSKSVFRCFAHRLFFIALLLFLPRGEAFAQDEGYRVLLLNSYHS
                     NFVWTAEVTDGIRQTLLSSGSEVEFLTEYMDTKRGFSVDALKAFSGYMERKYSGRSFD
                     LLICSDDDALVFLRRMGKRLFPDVPVIFCGVNSTSLYEPEAYPHITGLFENVDISGTA
                     ELALRLFPRTSALALVSDLSTTGMTVISQARSELAFLGDRVRIHDLFAFSEE"
     gene            742..>1098
                     /locus_tag="PK442_15280"
     CDS             742..>1098
                     /locus_tag="PK442_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOO89067.1"
                     /translation="MSLCYMNGTFLPKEKAMLPVSDYIILRGVGVFESISTFRKRPLM
                     LTPHLERLARSAESASITLPLPIEEIKAVVQEGLSRMPEDCLIRPYITGGDVFISGAF
                     PASRLFILFEKVQKPAP"
BASE COUNT          259 a          320 c          293 g          226 t
ORIGIN      
        1 cttcctccga gaatgcgaag agatcgtgga ttcggacgcg atcccccaaa aacgccagtt
       61 cgctccgcgc ctggctgatc accgtcattc ccgtggtgga gagatcgctc acgagcgcaa
      121 gagcgctggt gcgggggaaa aggcgaaggg caagctcggc ggtacccgag atatcgacgt
      181 tctcgaacag accggtgatg tgcggatagg cctccggttc gtacagcgaa gtgctgttca
      241 cgccgcagaa gattacggga acatccggga aaaggcgctt ccccatcctg cgaagaaaaa
      301 cgagcgcgtc gtcgtcggaa cagatgagca agtcgaagct cctgccggag tattttctct
      361 ccatataccc ggaaaaagcc ttcagagcgt ccactgaaaa acctcttttg gtatccatgt
      421 attcggttaa aaactcgacc tcggagccgc tcgaaaggag cgtctgccgg attccgtcgg
      481 tgacttcggc agtccagacg aagttgctgt gataggagtt gagcagcagc acgcgatacc
      541 cttcgtcctg cgcaaacgcc tctccgcggg gaaggaaaag aaggagcgcg atgaaaaaga
      601 gcctgtgggc aaagcatcgg aacaccgatt tcgacataac cgctctccct tcaaatcttt
      661 cgtcgaaaaa ccccgtgcat tttctctatg atacccttat actgcaaagg ttccaagacc
      721 gaaatttcga ggtgattttc aatgtccctg tgctatatga acggaacgtt cctgccgaag
      781 gagaaggcga tgcttccggt atccgattac atcattctcc gcggcgtcgg cgtctttgaa
      841 tcaatcagca ccttccggaa gcgtccgctg atgctgactc cccatctcga gcgcctcgcg
      901 cgttccgcgg agagcgcgtc gataacgctc ccgcttccga tcgaagagat aaaagcagtc
      961 gtccaggaag gactctccag gatgcctgag gactgcctga tccgcccata cattaccggc
     1021 ggcgacgtct tcatttccgg agctttcccc gcatccaggc tcttcatcct cttcgagaaa
     1081 gtccaaaagc ccgcgccc
//
LOCUS       DAOSWW010001041         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_128187_length_1098_cov_2.876318, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001041 DAOSWW010000000
VERSION     DAOSWW010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128187_length_1098_cov_2.876318"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PK442_15285"
     CDS             complement(<1..379)
                     /locus_tag="PK442_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO89068.1"
                     /translation="MLYRTMPQTGDKISILGFGCMRFPLDAEGKIDEPRSAAMLHSAI
                     DRGVNYIDTAYPYHGGESEPVVGRALEGGYREKVFLATKLPSWMVETPSDMDRLLDEQ
                     LTKLRTDHIDCYLLHSLNADRWGI"
     gene            complement(601..>1098)
                     /locus_tag="PK442_15290"
     CDS             complement(601..>1098)
                     /locus_tag="PK442_15290"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO89069.1"
                     /translation="ILVVADEIQAGLGRTGRYFAYEHFGLRPDIITLGKGIGGGLPLG
                     AAIFCEWSPFASGEHGSTFAPNPVSLAAGKATLAHITPSLLGDVSRKGERLRERLALL
                     SWARGTRGLGLMIGVSAENPAEVKKKAFERGVLLNVTGGGIRFLPALTMSDAEIEEIL
                     SLLNF"
BASE COUNT          222 a          348 c          320 g          208 t
ORIGIN      
        1 tgattcccca ccggtccgcg ttcagcgagt ggagcaggta gcagtcgata tggtccgtac
       61 gcagttttgt caactgctcg tcaagaagac ggtccatgtc cgacggcgtt tccaccatcc
      121 acgagggaag cttggtcgcg aggaagacct tctcgcgata tccgccttcg agcgcccggc
      181 cgaccacggg ttcgctctct cctccgtgat acgggtacgc cgtatcaatg tagttcacgc
      241 ccctgtcgat agcggaatgc agcatcgccg cagagcgcgg ctcgtcgatc tttccttcgg
      301 cgtcgagagg aaagcgcata cagccaaaac caagaatgga aatcttatcc cctgtctggg
      361 gcattgtacg atagagcata ttcaatacaa cctccttcgt atttcgccgc gcctgagaga
      421 agtgccggca cgaaaaacag gcgcgccatc tttccgtata tcatatatta tcgcatagac
      481 tttcctgttg cgcagtactc cgaataacag gaaggtccgg aatccggccc caagcggcac
      541 agattccgaa cctctctgat aaaggcggag tatgggggaa gcggcttccc ggcctcgtcc
      601 tcagaaattc agcagcgaca ggatctcttc gatctccgcg tcgctcattg tgagcgcggg
      661 gaggaatcgg atcccgccgc ccgttacatt gagcagcacg ccgcgctcga aggccttttt
      721 cttcacctcc gcgggattct ccgcgctcac gccgatcatg agccccagcc cgcgcgtccc
      781 gcgcgcccag gagagaagcg ccaggcgttc gcggaggcgt tcgcccttcc gggagacgtc
      841 gcccagcaga gagggtgtga tgtgcgcgag cgtcgctttg cctgcggcga gcgaaacagg
      901 gttcggggcg aaggtcgagc cgtgctcgcc ggatgcgaac ggcgaccact cgcaaaagat
      961 cgccgctccc aggggaagcc ccccgccgat ccccttcccg agagtgatga tatcggggcg
     1021 aaggccgaag tgctcgtaag cgaagtacct gcccgtgcgc ccaaggcccg cctgaatctc
     1081 gtcggccacc accaggat
//
LOCUS       DAOSWW010001042         1097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_128477_length_1097_cov_2.003839, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001042 DAOSWW010000000
VERSION     DAOSWW010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128477_length_1097_cov_2.003839"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PK442_15295"
     CDS             <1..769
                     /locus_tag="PK442_15295"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO89070.1"
                     /translation="LFLLGDQMQLAQPVQGTHPGKSGLSCLEHVLGEHSAVPPDRGVF
                     LEKSWRMHPELCAFVSDAFYEGRLRPLEGLERQEIRPIPGGHSIPWLVRGSGLIFVPV
                     AHSGNGRSSDEEVERIASLTNDLLRCVYIDAQGKEHAMEMNDILFVAPYNLQVAKLRR
                     RLGHEASVGSVDRFQGREAPVAILSMCSSSREESPRGMEFLCSPDRLNVAVSRARALA
                     IVVGSPDLLSGNFSSIREMELANLYAWIMEKGDGASF"
     gene            794..>1097
                     /locus_tag="PK442_15300"
     CDS             794..>1097
                     /locus_tag="PK442_15300"
                     /inference="COORDINATES: protein motif:HMM:NF025226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOO89071.1"
                     /translation="MKRCVVSSLLAIVIALCLPLVLAAKMSDEAFLELCKTGSVAQIQ
                     KAIRSGSDVNASDEWGSTPLHEAAWHNSNPDVLNVLLKAGANVNATTSSFVTPLHLA"
BASE COUNT          221 a          321 c          331 g          224 t
ORIGIN      
        1 gctcttcctt ctgggcgacc aaatgcagct cgcccagccc gtgcagggga cgcatcccgg
       61 aaagagcggc ctctcctgcc ttgagcacgt cctcggggaa cactccgcgg ttccgccgga
      121 ccggggcgtc tttctggaga agagttggag aatgcacccc gaactctgcg ccttcgtctc
      181 cgacgccttc tacgaaggca gactccggcc gctggagggg cttgagcgtc aggagatacg
      241 cccgatcccg ggcggacatt cgattccgtg gcttgttcgc ggctccggcc tgatcttcgt
      301 ccccgtggcg cacagcggaa acggccgatc cagcgacgag gaggtcgaaa gaatcgcctc
      361 tctgacgaac gacctccttc gctgcgtcta tatcgacgcg caggggaagg agcatgcgat
      421 ggagatgaat gatatcctct tcgtggcccc ctacaacctt caggtcgcga agctccggcg
      481 gaggctcggt cacgaagctt ccgtcggcag cgtggaccgc tttcagggga gagaggctcc
      541 cgtcgcgatc ctctccatgt gctcctcgtc gcgggaggag tcgccgagag gaatggagtt
      601 cctctgcagc cccgaccggc tcaacgtggc cgtctcgcgc gcccgagcgc tcgcaatcgt
      661 cgtcggatcg ccggatcttc tctccggaaa cttctcctcc atccgcgaaa tggagctggc
      721 gaacctgtac gcatggatca tggagaaagg cgatggcgcc tctttttgaa cgtggaaagg
      781 gaggtagact gaaatgaaac gatgcgtagt cagttcgttg ttggcaatag taatcgcgct
      841 gtgtctgccg ctggttttag cagcgaagat gagtgatgaa gcctttcttg aactttgcaa
      901 aacaggttcc gtagcgcaga tccagaaggc gatacggagt ggatctgatg tgaatgcaag
      961 cgatgagtgg ggctctaccc cactgcatga agctgcatgg cacaactcca accctgatgt
     1021 gctaaatgta ctgctgaaag ccggagccaa cgtaaacgca actaccagta gctttgttac
     1081 tccgctgcat cttgcag
//
LOCUS       DAOSWW010001043         1095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_128792_length_1095_cov_2.776923, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001043 DAOSWW010000000
VERSION     DAOSWW010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128792_length_1095_cov_2.776923"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..568)
                     /locus_tag="PK442_15305"
     CDS             complement(95..568)
                     /locus_tag="PK442_15305"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOO89072.1"
                     /translation="MSKNFMSGLLSLVLGAIYFFTATQFPEVTAGDRTGPKLFPLIIG
                     SVAMIAGGLLCVLDRRSGKGQPIEWNFVADRAVWSKILVIMLLGISYGLVLDSWGYLV
                     ATTVFMFCITLLINRRHFLQNLLISIFFPVVTYAAFAVALQLSLPRGIIENMLPF"
     gene            complement(654..>1095)
                     /locus_tag="PK442_15310"
     CDS             complement(654..>1095)
                     /locus_tag="PK442_15310"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOO89073.1"
                     /translation="GTNPDMTGPAEGGEIVPALLGGHVKATTIGLGEVLEQHKAGKMR
                     IIGVSADQKLDFLPDVDTFTAQGVNLVFPHWRGIIGAPDLTPEQVKYWDDVFTKMVQT
                     ETWKTLIANLGWSNFYQNSAEHTTFLEKSTTDFDKLLTQVGLKK"
BASE COUNT          235 a          294 c          321 g          245 t
ORIGIN      
        1 tccgatgaag acccacagta tgttgtaggc cgtgatggcg gtggagaatc ctattgcgag
       61 attttgaaag atagcgtcca tgtgtgccga cctcctagaa ggggagcatg ttctcgatga
      121 tgccgcgagg gaggctcagc tgaagagcca ctgcgaaggc cgcgtatgtg accacgggga
      181 aaaagatgga gatgagaaga ttctgcaaga agtgcctccg gttgatgagc agcgtgatgc
      241 agaacatgaa aaccgttgtg gcgacaaggt atccccaaga gtcgaggaca agcccgtaag
      301 agattcccag cagcatgatg acgagaatct tcgaccacac cgcgcggtcc gcgacaaaat
      361 tccactcgat cggttgccct tttccgcttc ttctgtccag gacgcagaga agccctccgg
      421 cgatcatggc gacgctcccg atgatcagag ggaagagctt cggccctgtt ctgtctcctg
      481 ccgtaacttc aggaaactgc gtcgccgtaa aaaaatagat tgctcccagg acgagagaga
      541 gcagtccgct catgaagttt ttgctcattc tgcaacctcc gttttctgtt gtttcttcag
      601 aaaagaaaga cggcgggaaa atccgtcgat ctccccgccg aaaggaatac gagctacttc
      661 ttcagcccaa cctgcgtcag cagcttgtcg aagtccgttg tgctcttctc gaggaaggtc
      721 gtatgctcgg cggagttctg gtagaagttg ctccacccga gattcgcgat aagggttttc
      781 caggtctcgg tctgcaccat ctttgtaaag acgtcgtccc agtacttcac ctgctccggc
      841 gtcagatcgg gagcgccgat gatgcctctc cagtgcggga agacgaggtt gacgccctgc
      901 gctgtgaagg tgtcgacgtc cggaaggaag tcgagcttct ggtcggcgct cacgccgatg
      961 atccgcattt tgccggcttt gtgctgttca agaacttcgc cgaggcctat tgtcgtggcc
     1021 tttacgtggc cgccgagaag cgccgggacg atctctccgc cctctgccgg gccggtcatg
     1081 tcggggttgg ttccg
//
LOCUS       DAOSWW010001044         1091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_129610_length_1091_cov_3.194015, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001044 DAOSWW010000000
VERSION     DAOSWW010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129610_length_1091_cov_3.194015"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK442_15315"
     CDS             complement(<1..618)
                     /locus_tag="PK442_15315"
                     /inference="COORDINATES: protein motif:HMM:NF020584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HOO89074.1"
                     /translation="MKDGFITEARREFHLRILSGIVRTNAKGTPNFADSSSVLSSSIA
                     REMLSLFGGSAGEGLLTAQTAGLVFEDLCLSFLRDCFEKIAHLRPGKWMFARNLSIAR
                     FEQYKHLVDVENLTALHPELAAVLGGNYIIKPDIVVSREPEEDDMINMSGSVVDALSA
                     NRTVLRKANGTDPILHASVSCKWTLRSDRSQNSRTEALNLIRNRKG"
     gene            complement(615..>1091)
                     /locus_tag="PK442_15320"
     CDS             complement(615..>1091)
                     /locus_tag="PK442_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOO89075.1"
                     /translation="EGTEIRDEEPPLDLHFGRDPTKTSRERYEAVPPGGNRFDLQKNA
                     PELLPECWRKKTSGGTDLFGRLWWDRPSVTIRTEFFKPEKGRYLHPEKHRPITHREAA
                     RLMGFPDDFLFRGSKTEIARQIGNAVPPPLAAAVARQVKEALDSISESKEGARKVS"
BASE COUNT          211 a          322 c          313 g          245 t
ORIGIN      
        1 ccccttcctg ttgcgtatca ggttcagcgc ctccgtcctt gaattctggc tccggtcgct
       61 ccgcagggtc cacttgcacg agacgcttgc gtgaaggatg ggatccgttc cgttcgcttt
      121 ccgaagtacg gtcctgtttg ccgagagtgc atcgacaaca ctccccgaca tattgatcat
      181 atcgtcctct tcaggttcac gggaaacgac tatatcaggc ttgatgatgt aatttccgcc
      241 caggaccgcg gcaagttccg gatgcagcgc cgtgagattc tccacgtcca caagatgctt
      301 gtactgctcg aatcgtgcta tggagaggtt gcgcgcgaac atccatttgc cggggcgcag
      361 atgggctatt ttttcaaagc agtcccgaag aaacgaaaga cagagatctt cgaagacgag
      421 cccggccgtc tgtgcggtca gaagcccttc gccggccgac ccgccgaaaa gagacagcat
      481 ctcgcgggca atggaagagc tgagaacact cgaactatcg gcaaagttgg gggttccttt
      541 ggcgttcgtc ctgacgatgc ccgaaagaat ccggaggtga aactccctcc gggcttccgt
      601 tataaaaccg tctttcatga gacttttcgc gccccttcct tggattccga gatgctgtcg
      661 agggcttctt tgacctgcct cgctacggcc gccgccagag gcggcggtac ggcgttacct
      721 atctggcggg ctatctcggt cttactgccg cggaagagaa aatcgtcggg gaaacccatg
      781 agacgcgcag cctcgcggtg ggtgatggga cggtgtttct ccgggtgaag atagcgccct
      841 ttctccggct tgaaaaactc cgttcggata gtgacggacg gacgatccca ccaaagacgc
      901 ccgaagagat ccgtgccgcc ggaggtcttc tttcgccagc attccggaag caactcaggc
      961 gcgttcttct gcagatcgaa gcggttgccc ccgggaggca cagcctcgta tcgctccctg
     1021 cttgtcttcg tcgggtccct cccgaaatgg aggtcgagag gcggctcttc gtcgcgtatc
     1081 tcggttccct c
//
LOCUS       DAOSWW010001045         1090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_129813_length_1090_cov_3.312077, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001045 DAOSWW010000000
VERSION     DAOSWW010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129813_length_1090_cov_3.312077"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..661)
                     /locus_tag="PK442_15325"
     CDS             complement(128..661)
                     /locus_tag="PK442_15325"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOO89076.1"
                     /translation="MDARSAKPIVGILTSRTDLLRDFLGMLSDFFGREDIVGDWLAFD
                     HTRYYEEEMGSGLLRTFVSFERHTAGEDAAQFKKWTRIVEDDFRRGGKRTVNLDAGTL
                     DANKVVLVSGKHGGHKIALAPGVWADIMLWYNRGWVALPWAFPDFRDGKLFPLFTEMR
                     KRYKDAVNSEPPPTSPS"
     gene            complement(634..>1090)
                     /locus_tag="PK442_15330"
     CDS             complement(634..>1090)
                     /locus_tag="PK442_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89077.1"
                     /translation="LPLPEVGESAMSGAASSLRLLRLGKSSHLLLFTDRMGTSRLRYR
                     MRDVPVSACDFRRRWVDSFEWAMMRHLCVSRIIDGKQVYMRDCTIRRIETDRSRQESQ
                     RRGLASKAANSFGLEIGLVNAARDCLVRSSEKYRREKGGRIWTPDQQSR"
BASE COUNT          202 a          340 c          324 g          224 t
ORIGIN      
        1 gcggcccgga cgcatgcggc taaggcggaa gcgctcatag acggggcgaa ggtgatgacc
       61 gggggcgaga cagccgcctc atcgtccggc gctgccgtct cagttcctcc agcggtctcc
      121 gggaaggtca gctcggggaa gtaggcggtg gctctgaatt gacggcgtcc ttgtaccttt
      181 tccgcatctc cgtgaagagc gggaagagct ttccgtccct gaagtcgggg aaagcccatg
      241 gcagcgcgac ccaaccccta ttgtaccaga gcatgatatc cgcccacacg ccgggcgcga
      301 gagctatctt gtggccgccg tgctttccgg agacgaggac gaccttgttc gcgtcgagcg
      361 tgccggcgtc caggtttacg gttctttttc cgccgcgcct gaaatcgtcc tctacgatgc
      421 gcgtccattt cttgaattgc gctgcgtctt ccccggccgt atgacgctcg aacgagacga
      481 acgttcgcaa cagtccgctt cccatctcct cctcgtagta gcgagtatgg tcgaatgcga
      541 gccagtcgcc cacgatatcc tcgcggccga aaaaatccga caacatcccc aaaaagtccc
      601 ggaggaggtc ggtcctcgag gtgaggatac ccactatcgg ctttgctgat cgggcgtcca
      661 tatccttcct cccttctccc gtcggtattt ctccgaagat cgaacaaggc agtcccgcgc
      721 ggcgttaacg aggcctattt ccaaaccgaa gctgtttgcg gcctttgagg cgagtcccct
      781 tctttgtgat tcctgacgcg agcgatccgt ttcgatcctt cgaatggtgc agtccctcat
      841 atatacctgc tttccgtcaa tgatgcgcga cacgcagagg tgcctcatca tcgcccattc
      901 aaaggagtcg acccagcgcc tgcggaagtc gcaggcgctg accggaacgt cccgcattcg
      961 gtagcggagc ctggaggtcc ccatgcggtc ggtgaagagg agcagatggc tgctcttccc
     1021 gaggcgaagg agccggagcg acgatgctgc gccgctcatc gcggattcac cgacttccgg
     1081 caaggggagg
//
LOCUS       DAOSWW010001046         1088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_130235_length_1088_cov_3.056147, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001046 DAOSWW010000000
VERSION     DAOSWW010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130235_length_1088_cov_3.056147"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PK442_15335"
     CDS             <1..251
                     /locus_tag="PK442_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89078.1"
                     /translation="REESAAEGPGSPSPVEAMESLFNLLEEQRPEGLLGMRRIPSCPS
                     LNRGVMVAEPMDRVPWKTAFVRWMRRWRRELFAREGAR"
     gene            248..>1088
                     /locus_tag="PK442_15340"
     CDS             248..>1088
                     /locus_tag="PK442_15340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO89079.1"
                     /translation="MNTTDTAAVPSWRRSGSRRRIALLFLVLIPTITSSYTMARLLPE
                     RASLVLDALLVAVFGILFAWISVGFWAFMGGLFVLLTKRDRYRVSKSCDGRDLSIPDA
                     NARTAILIPIYNEEVNRVFAGVRTVYRSLKQTGALDRFDIFLLSDSTSPDVWVEEEEA
                     WHDFRREEEGTEAHVFYRKRDNNIKRKSGNVADFCRRWGKNYRYMIVFDADSVMTGES
                     MVRLVQIMEDRSDIGIIQTLPLAVNRETLIARVQQFANHVQGPLLAAGLHFWQLGDAQ
                     YCGH"
BASE COUNT          198 a          371 c          339 g          180 t
ORIGIN      
        1 cgcgcgagga atccgctgcg gaaggcccgg gctcaccgtc ccccgtcgag gcgatggagt
       61 cgctcttcaa cctgcttgaa gagcagcgtc cggaagggct gctcggcatg cggcgaatcc
      121 cctcgtgccc ttcgctgaac aggggcgtga tggtcgccga accgatggac cgcgtcccgt
      181 ggaagacggc gttcgtccgc tggatgcggc gctggcgccg tgaactcttc gccagggagg
      241 gcgcgagatg aacacaaccg acaccgccgc cgtcccctcc tggagacgtt ccggaagcag
      301 acggcgcatc gcgctgctct tcctcgtcct cattcccaca atcacgtcga gctacacaat
      361 ggcccgcctg ctccccgaac gcgcgagcct cgtactcgac gcgctcctcg tagccgtgtt
      421 cggcatcctc ttcgcgtgga tttccgtggg cttctgggcc tttatgggag ggctcttcgt
      481 tctcctgacg aagcgcgacc gttatcgcgt ctcgaagagc tgcgacggac gcgacctctc
      541 cattcccgac gcgaacgcga gaaccgcgat cctcatcccc atctacaacg aagaggtgaa
      601 ccgcgtcttc gcgggggtgc gcaccgtcta ccgctcgctg aaacagacag gcgccctcga
      661 ccgcttcgac atcttcctcc tcagcgacag caccagtcca gatgtctggg tggaagagga
      721 ggaggcgtgg cacgacttcc gccgcgagga ggaggggacg gaggcgcacg tcttctaccg
      781 gaaacgggac aacaacatca agcggaagag cggcaacgtg gcggacttct gccgccgctg
      841 ggggaagaac taccgctaca tgatcgtctt cgacgcagac agcgtcatga ccggcgagag
      901 catggtccgc ctggtgcaga tcatggagga ccgctccgac atcggcatca tccagacgct
      961 cccgctggcc gtcaaccgcg agacgctcat cgcgcgcgtg cagcagttcg ccaaccacgt
     1021 ccaggggccg ctgctcgccg cagggctgca cttctggcag ctcggcgacg cacagtactg
     1081 cgggcaca
//
LOCUS       DAOSWW010001047         1088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_130259_length_1088_cov_2.703775, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001047 DAOSWW010000000
VERSION     DAOSWW010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130259_length_1088_cov_2.703775"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PK442_15345"
     CDS             <1..209
                     /locus_tag="PK442_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89080.1"
                     /translation="GHYHLQSRPFEAGANGLFRAEYREVRPLSVSERMKKALATNDFK
                     DWKIDFDEMIEDEGMNPKEPLGGW"
     gene            complement(254..946)
                     /locus_tag="PK442_15350"
     CDS             complement(254..946)
                     /locus_tag="PK442_15350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO89081.1"
                     /translation="MEKTHGIFPVLNIDDRTWFCADHHFGHRSVLKFDRRPYADLDEM
                     ERDLVMKHNATVPPNGATVIFLGDVVYPKGDRETDWRDIVRSLHGDRKILLAGNHDAS
                     LEKRFSKVFDAVLPEGVPLTVRLRGREVRCVHSPENLCKRICPGFDDLPSGYADVLLT
                     LEYGNERIEGEWLCGHVHTVFRKLGSMVNVGVDAWDYAPVSVADAFALLDDPRIGIPG
                     RKGFDGDFGEAG"
BASE COUNT          227 a          339 c          307 g          215 t
ORIGIN      
        1 tcggccacta ccatctccag tcacgtcctt tcgaggcggg tgcgaacggc ctattccgcg
       61 cggagtacag ggaagtccgc ccgctctccg tatccgaacg catgaagaag gcgcttgcga
      121 cgaacgactt caaggactgg aagatcgact ttgatgagat gatcgaggac gagggaatga
      181 atcccaaaga accgctcggg ggatggtagc ggagtaggct ctcgtacctc cctccaaagc
      241 gcgctccccg tcctcaaccc gcttctccga agtccccgtc gaaccctttt ctccccggaa
      301 tgccgatcct cggatcatcc aaaagagcga aggcgtcggc aacggagaca ggggcgtagt
      361 cccacgcgtc gacaccgacg ttgaccatgg aaccgagctt ccggaagacc gtatgtacgt
      421 ggccgcagag ccactcccct tcgatccgtt cgttcccgta ctcaagcgtc agaagaacgt
      481 cggcgtagcc ggagggaagg tcgtcgaaac cggggcatat tctcttgcaa aggttttctg
      541 gggagtgaac gcaccgtacc tcccgccctc ggagccgcac cgtcaagggg actccctcgg
      601 gcagcacggc gtcgaagact ttggaaaagc gcttttcaag ggatgcgtcg tgattgcccg
      661 cgagaagaat cttcctgtct ccgtgaagag agcggacaat atccctccag tccgtctccc
      721 tgtccccctt ggggtagacg acgtcgccga ggaagataac cgtcgcgccg ttcggaggta
      781 cagtcgcgtt gtgcttcatc acaaggtcgc gctccatctc gtcgagatcc gcatacggac
      841 gccggtcgaa cttcagcacg ctccggtgac cgaaatgatg gtccgcgcag aaccatgttc
      901 tgtcgtcgat attgagaacg ggaaatattc cgtgcgtttt ctccatatcc gtcactcctc
      961 caacggtttc ctcaagatga aagcaagccc gtcgccgcgg tagttgagaa caagctcttt
     1021 cgggaaaccg gtttgctcga ccagcgtctc gcagtttgcg agcgctcccg cgtcgcctcc
     1081 ccagtgcg
//
LOCUS       DAOSWW010001048         1087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_130468_length_1087_cov_2.680233, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001048 DAOSWW010000000
VERSION     DAOSWW010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130468_length_1087_cov_2.680233"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PK442_15355"
     CDS             <1..437
                     /locus_tag="PK442_15355"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO89082.1"
                     /translation="IVAGLGSYEVADAFHKNTWEHAAGDFQVKADSSIDYEITEPCVV
                     AVFQSNSWRYDGKGSPNGGRRDLLVDGSQRLIPTAAIKAPTLLIVGDKDPYVSVALCE
                     EAKATLTAEGSQLKVIPGASHAMMMEKPFYKEFRETVLEFLK"
     gene            complement(557..>1087)
                     /locus_tag="PK442_15360"
     CDS             complement(557..>1087)
                     /locus_tag="PK442_15360"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HOO89083.1"
                     /translation="ISVVTGISLHQIEAKLGKRMRIARAMPNTAIAVRESMTCIASNG
                     PDDKALEMAKLLFNDVGKTLRIREEDMLAATALGACGIAYFLRAIRAASQGGIEVGLS
                     AKNSLAMAVQTAKGAACLLSVGGTHPESEIDKVTTPNGCTISGLNCMEHEGFSSAMIR
                     GITVSAEKAARLYRKE"
BASE COUNT          192 a          351 c          340 g          204 t
ORIGIN      
        1 ctatcgtcgc cggactcggc tcgtacgaag tcgccgacgc cttccataag aacacgtggg
       61 agcacgcagc gggagacttc caggtaaagg cggacagctc tatcgattac gagatcaccg
      121 agccctgcgt ggtcgccgtc ttccagtcca acagctggag atacgacggg aagggaagcc
      181 ccaacggagg acgccgcgat cttctcgtcg acgggtctca gcgcctcatc cccactgcgg
      241 cgatcaaagc cccgacgctt ctgatcgtcg gcgacaagga cccgtacgtc tcggtcgcgc
      301 tctgcgaaga ggcgaaggcg acgctgacgg ccgaggggtc gcagctgaag gtcattcctg
      361 gagcttcgca cgcaatgatg atggagaagc ctttttacaa ggagttccgc gagaccgttc
      421 ttgagtttct gaaatagcgg aggcgcccgc cctgcggggc gtgcggcttc cgttcagaag
      481 cagacaacgc tttcggcgcc ggaacatcca accccggtcc gaaagcgttg ttcattccgt
      541 ttttcattcc tccgcgctac tctttccggt agagcctcgc ggccttctcc gcggagacgg
      601 tgatgccacg tatcatcgcg gagctgaacc cctcgtgctc catgcagttc agtccggaga
      661 tggtgcagcc gttcggcgtc gtcaccttgt cgatctcgct ctccgggtgc gtaccgccca
      721 cggagagaag gcacgccgct cccttcgcag tctgcacggc catcgcgagg gaattcttcg
      781 cggagaggcc gacttcgatg ccgccctgcg acgcggcgcg gatcgcccgc aggaagtagg
      841 cgataccgca cgcgcccagc gcggtggcgg cgagcatgtc ctcctcgcgg atgcggagcg
      901 tcttccccac gtcgttgaag agcagcttcg ccatctccag cgccttgtcg tccgggccgt
      961 tggaggcgat gcaggtcatc gactccctca cagcgatggc ggtgttcggc atggcgcggg
     1021 cgatgcgcat ccgcttgccg agcttcgcct cgatctgatg gagggaaatg cccgtgacca
     1081 ccgagat
//
LOCUS       DAOSWW010001049         1087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_130533_length_1087_cov_2.050388, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001049 DAOSWW010000000
VERSION     DAOSWW010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130533_length_1087_cov_2.050388"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PK442_15365"
     CDS             complement(<1..1028)
                     /locus_tag="PK442_15365"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOO89084.1"
                     /translation="MKNVQGFFEERGLLSPESRINELLRVKTVLNAVANEKVAKEDEE
                     TLFLAMKELTEKEFGFFPADRSDFRELFETLKRVDALEFAADVFAEDRKSLLGSPLLT
                     RYVVERIESLKPKSVLIAEAEKHLAGLGEMRRKCEGIELTLTTENVAVYRLLSLEFAE
                     TPDVIVRQESIYKESPDALKYEYIHAFPAVGRRREAGEGTGKTIPANELDEIAVENLL
                     RRLAPGGTMDVVVPAKMTFGGGGYKKLRSLLAESFGVVSLFNLPEKLFRPVIALRLCL
                     LSVSERKRDTVLIGAYEEDGDSLAVTLKKEMSSREFLSRGDWRVELLLSEEDEAVRRF
                     RESAVRKV"
BASE COUNT          218 a          352 c          272 g          245 t
ORIGIN      
        1 ctgacctttc gaaccgcgga ctctctgaag cgccgtacag cctcgtcctc ctccgagaga
       61 agaagctcga ccctccagtc gccgcgcgag agaaactccc gcgaagacat ctcctttttc
      121 agcgttaccg caagcgaatc cccgtcctct tcgtatgcgc cgatcaggac ggtgtcccgc
      181 ttcctctcgg agacggagag aagacagagc cgcagagcga tgacggggcg aaagagcttc
      241 tccggcagat tgaagagaga tacgaccccg aagctctccg ccaggaggga acggagcttc
      301 ttgtatcctc cgccgccgaa ggtcatcttc gccggaacga ccacatccat cgttcctccg
      361 ggagcgaggc ggcgcaggag attctcgacg gcgatttcgt ccagttcatt cgccgggatc
      421 gtctttccgg tcccctcgcc cgcctctctc ctgcgcccga cggcgggaaa agcgtggata
      481 tattcgtatt tcaaagcgtc gggactctcc ttgtagatgg attcttgccg gacgatcaca
      541 tccggcgttt cggcgaactc cagagagagc agtctgtaca cggcgacgtt ctcggtcgtc
      601 aacgtcagtt cgatgccctc gcacttccgc cgcatctccc ccagtcctgc cagatgtttt
      661 tcggcctcgg cgatcaggac gctcttcggc ttcagcgact ctatcctttc gacgacgtag
      721 cgagtcagaa gcggcgaccc gagcaacgat ttacgatctt cggcgaagac gtccgccgcg
      781 aattcgagag catcgactct cttcagtgtc tcgaagagtt cccggaaatc cgagcggtcc
      841 gcggggaaga atccgaactc cttctccgtc agctccttca ttgcgagaaa gagcgtctct
      901 tcgtcttctt tcgcgacttt ctcgttcgcg accgcattca gcaccgtctt cacccgcagc
      961 agttcgttta tccggctttc aggcgagagg agccccctct cttcaaaaaa accctgtacg
     1021 tttttcatag tcttcgcctc cttcaatcca aatagcctac acccttaaat ttagtatatc
     1081 aatacta
//
LOCUS       DAOSWW010001050         1086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_130645_length_1086_cov_3.192047, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001050 DAOSWW010000000
VERSION     DAOSWW010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130645_length_1086_cov_3.192047"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PK442_15370"
     CDS             complement(<1..259)
                     /locus_tag="PK442_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89085.1"
                     /translation="MRKFFVALLAIVLLFGGAYGALAADEFTTLSFSPGGMVKGRMPV
                     KITFSQPVVEKSLVGKTVPLRNIPVEFSPSIRGAGKWTDQKT"
     gene            complement(556..>1086)
                     /locus_tag="PK442_15375"
     CDS             complement(556..>1086)
                     /locus_tag="PK442_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89086.1"
                     /translation="GGKDTWAILSMETEYYDNEGQVSDEAVEGLWIEVPNFPAEAEVV
                     DFQMNDEGVASFTRILDAGVVALVVERLPVTNTEGEQVTPQTLGKLVAEFEGIEEEKI
                     EITPSLDDFAKLYSYPVAGAEFTTGENEDTRKVVDLFLFTDSWLFRIHVSVTADSFEE
                     YEDQVKEWLSSLKFRE"
BASE COUNT          251 a          302 c          263 g          270 t
ORIGIN      
        1 acgtcttttg atccgtccat ttgcccgctc cgcgtatgga aggcgaaaac tcaaccggta
       61 tatttcgcag cggcacggtt ttgccgacga ggcttttctc cacgacaggc tgcgagaagg
      121 tgatctttac cggcatcctg cccttgacca tccctccggg agaaaacgag agagtggtga
      181 attcatcagc cgctaacgct ccgtacgctc ctccgaagag taaaacaatc gcgagaagcg
      241 ccacaaaaaa ctttctcata tgtatcatct ccttcttctc tgaaatatat ggtagtgcgc
      301 cgtgtatgtt gtttcttttg gaattatacc gcgaatcgcg gagaagaggg gcatggtagc
      361 gggaagaata cgaaatacac gggagctcct acagaaaagc gaagagaaga cattctgtgc
      421 cgggctgtta cgttcaccca aaacaatggg tttcagcaat cgcgcagacc gagtacgtcc
      481 ttctccccgc tcttcatccg aggggttttc ggtgctggag cctacccctc atgcgtatag
      541 tgatttttta ctaagctatt ccctgaattt caggctcgac aaccactctt ttacctgatc
      601 ttcgtactcc tcgaaagagt ctgctgttac cgacacgtgg attcggaaca gccaagaatc
      661 agtgaaaagg aagagatcga cgaccttgcg ggtatcctcg ttttctccag tcgtgaactc
      721 ggccccggcg acggggtagg agtacagctt tgcaaaatcg tcaagagagg gggtaatctc
      781 tattttctct tcctctatcc cttcaaactc agcgacaagc ttcccgagcg tttgtggcgt
      841 cacctgttcg ccttcggtat tcgtgacagg cagtctttcc acgacgagtg caacgacgcc
      901 tgcgtccagg atgcgcgtaa acgaggcaac gccttcgtcg ttcatctgga aatcaacgac
      961 ttccgcttcc gcggggaagt taggaacctc gatccacaat ccctccacag cctcgtcgga
     1021 aacctgtccc tcattatcgt agtactctgt ttccatgctg aggatcgccc aggtatcctt
     1081 gccgcc
//
LOCUS       DAOSWW010001051         1086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_130661_length_1086_cov_2.943744, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001051 DAOSWW010000000
VERSION     DAOSWW010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130661_length_1086_cov_2.943744"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1086)
                     /locus_tag="PK442_15380"
     CDS             complement(<1..>1086)
                     /locus_tag="PK442_15380"
                     /inference="COORDINATES: protein motif:HMM:NF037277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO89087.1"
                     /translation="ILFLYILLTRRWSLVRRVLYIPGILLFFALTVPWFVEVCRRNSD
                     FFYFFFIQEHFLRYATTMHGRYAPMWFFIPILIVGFFPWAGLLPGAIGSAIPLPLRTA
                     DREKRNELFLILWFGVIFVFFSISSSKLIPYIVPAAPPLAILMGRYLSRCIEEEDVKK
                     TGRFLLWNSLLLFPFIGALLVYPFFNDRIAAGRLLPYSLPIAAVLILFVFSGWHSFLR
                     RDFRRLALFLCVLSFANMFAFSRVFLLYDSLLTARELAGRIAEIRNDGDIVAQFANYD
                     QGLPFYLKQRIVLVNYMGELEFGALQEADPSWFIDGKGLAELWTGDRRVILVVNSDHK
                     EYVAQQTGLSLPEPSAETAKRLVFVNR"
BASE COUNT          273 a          288 c          356 g          169 t
ORIGIN      
        1 tgcggttgac gaagacgagg cgctttgcag tctcggcgga aggttccgga agcgaaagcc
       61 cagtctgctg cgcgacatac tccttgtggt cgctgttgac gaccaggatg acgcgccggt
      121 cgcccgtcca aagctcggcg agccccttcc cgtcgatgaa ccaggacgga tcggcctcct
      181 gaagcgcccc gaactcaagt tcccccatgt agttgaccag cacgatccgc tgtttcagat
      241 agaaggggag cccctggtcg tagtttgcga actgggcgac gatgtcgccg tcgtttcgga
      301 tttcggcgat tcttcccgcg agttcgcgcg cagtaagcaa ggaatcgtag agaaggaaga
      361 ccctgctgaa ggcgaacatg ttcgcgaagg agagcacgca gaggaagagg gcaagtctcc
      421 tgaaatccct gcggaggaag ctgtgccacc cggagaagac aaacaggatc aggaccgctg
      481 cgatcgggag cgagtagggg agcagacgtc cggcggcgat ccggtcgttg aagaacggat
      541 agacaagcag cgcgccgata aagggaaaca gcaacagcga attccacagc aggaagcgtc
      601 ctgtcttctt gacgtcctcc tcctcgatgc agcgggagag ataccgcccc atcagaatcg
      661 cgagcggcgg cgctgcgggg acgatatacg gaatcagctt cgaactcgaa atagagaaga
      721 agacgaagat cacgccgaac cagaggatga gaaaaagttc gttccgtttc tcgcgatcgg
      781 cggtccgcag cggcagcggg atcgccgagc cgatcgcgcc ggggagcagc cccgcccagg
      841 gaaagaaacc gacgatcaga atggggatga aaaaccacat gggcgcgtac cttccgtgca
      901 tcgtcgtcgc gtagcgcagg aagtgttcct ggatgaaaaa gaagtagaag aagtcgctgt
      961 ttctgcggca gacctcgacg aaccagggga cggtgagcgc gaagaaaagg aggattccgg
     1021 gaatgtagag cacccggcgg acaaggctcc agcggcgtgt gagcagaata tacaagaaga
     1081 ggatgc
//
LOCUS       DAOSWW010001052         1085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_130989_length_1085_cov_1.793204, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001052 DAOSWW010000000
VERSION     DAOSWW010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130989_length_1085_cov_1.793204"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..457
                     /locus_tag="PK442_15385"
     CDS             206..457
                     /locus_tag="PK442_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89088.1"
                     /translation="MELVLVGNGFQRGSHFVFRHRLIVKYCRMLPGYFLGVFAPDGSL
                     LIVSHGNQVKEWYLRKAADALEKLQEIEILEANLQKCAV"
     gene            675..917
                     /locus_tag="PK442_15390"
     CDS             675..917
                     /locus_tag="PK442_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89089.1"
                     /translation="MPDKKDALIVLLKFLNNPLVDGTRNLTEPDMEEFAKQNPAMAES
                     MLDLAVIAEEAEEVLRASGVYVREEPDVEDMIDWIN"
BASE COUNT          264 a          268 c          333 g          220 t
ORIGIN      
        1 atgatcccct ccgggatggt gaactgatcc gcgcggctgt ctcgcaagcg gggagtcctt
       61 cagcattccc gttctcctgc gcatagggaa aaccctgcga agtatctgaa ccggtatccg
      121 agagaaggca ggtgtttttt ctttgacgtc gaaggaaaaa agcatcgagc gctggaagag
      181 ggtaaaggag gagaggtacg cctcggtgga attggttctt gtcggcaacg gatttcaaag
      241 gggctcccat ttcgtcttcc gccaccgtct tatcgtgaaa tactgcagga tgctgcccgg
      301 ctactttctc ggtgttttcg cgcccgacgg ttcgctgctc atcgtcagcc acgggaacca
      361 agtcaaagag tggtatctga gaaaggccgc agacgccctg gagaaacttc aggagataga
      421 gatcctagaa gcgaatctcc agaaatgcgc cgtgtagaaa ggtgagaggg gggtgttctc
      481 cctcgttttt tcgagaaatc cgaagaagag gggcctcctc ctcttcggat ttctctttcc
      541 atcctgaatg cagaaatcca tcggccagag gaatccgaaa aaatgccggg gagttcggga
      601 tggagaagat tctcccgaac ggataatgct tcttcagggt atccgaagtg aaagccgcga
      661 ggagggatcg tacagtgccg gacaaaaagg acgcgctgat cgtgcttctg aagttcctga
      721 acaatccgct tgtggatgga acacgcaacc ttacggagcc cgatatggag gaattcgcga
      781 aacagaaccc cgcgatggcg gagtcgatgc tggatctcgc cgtcatcgcg gaggaggccg
      841 aagaggttct gagggcatcg ggagtttatg tccgcgaaga accggacgtg gaggatatga
      901 tagattggat caactgagcc gttattcggg aggagatgga aaccgatgat agtctccgaa
      961 gagcaacgcc gaatcgtcga actcgaccac gaatcgcaca tcgtccttgc gccgccggga
     1021 acgggcaaga ccgagctgct cgccagacgg gcggagcacg cattgaagcg cggagttccc
     1081 tcgga
//
LOCUS       DAOSWW010001053         1084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_131094_length_1084_cov_2.781341, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001053 DAOSWW010000000
VERSION     DAOSWW010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131094_length_1084_cov_2.781341"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1084
                     /locus_tag="PK442_15395"
     CDS             <1..>1084
                     /locus_tag="PK442_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89090.1"
                     /translation="NIEGFNTLAEGMKELRRMEYLADLAETLAMLQEEKDAIAALGEE
                     AGNGAAVMKRNYDTWRKAISEYDRLKKSLVRGYSYFYGKRRVPGWLVIKSDADSVQAP
                     DRTIPEEFGPELGTLQRMKTSIASDLRRLPQVKGDERRFRSTLEKIKKTYNALLPGYE
                     AARESLEKTNLALGKLLSVIGSAPTPTSAEEKETPPPDSGGEDTAFGLRIPLDLHGML
                     LEMTGLTGIPEGTHKGREDVEHGYYLTEETLNSDYQIVFSTPDKKEIKVSLKGARGLP
                     DPGQNRFLALAGTIAYSKKKSVVGYYLDFKNNKLERPPQGVYEGLVVMNYAVFDAKAT
                     DDPAGTLEFHAARFGLGKGTTPEMLKG"
BASE COUNT          261 a          297 c          345 g          181 t
ORIGIN      
        1 ggaatatcga gggtttcaac acgctcgccg agggaatgaa ggagcttcga cggatggagt
       61 acctggccga cctcgcggaa acgctcgcta tgctgcaaga ggagaaggac gcgatcgcgg
      121 ctctgggcga agaggcaggg aacggcgccg ccgttatgaa acggaactac gatacctgga
      181 gaaaggcgat ctccgaatac gacaggttga aaaaatccct tgtcaggggc tactcctact
      241 tctacggaaa gaggcgcgtt cccggatggt tggtgatcaa gagcgacgcg gactcggtcc
      301 aggctcccga caggacgatc cccgaggagt tcgggcccga actcggaacg ctgcagagga
      361 tgaaaacctc catcgcctcg gacctgcgac ggcttcctca ggtgaagggg gacgagcggc
      421 gtttccgttc gacgcttgag aaaatcaaaa agacgtataa cgcgcttctt cccggctacg
      481 aggccgcacg agaatccctt gaaaagacga atctcgcgct cggaaaactg ctttcagtga
      541 tcggaagcgc tccgacgccg acgtcggctg aagagaagga gacccctcct cccgactccg
      601 gaggggagga tacagcattc gggctccgca tcccgctcga cctgcacggc atgttgctgg
      661 agatgacggg gcttaccggc attccggagg gcacgcacaa ggggcgggaa gacgtcgagc
      721 acggctacta tctcacggag gagacgctga attccgacta ccagattgta ttctcgacgc
      781 cggacaagaa ggagatcaag gtcagcctga agggagcgcg cggtcttccg gatccgggac
      841 agaaccgttt cctcgcgctt gcggggacga tagcctactc gaagaagaaa tccgttgtgg
      901 gctactacct cgacttcaag aacaacaagc tggagcgccc tccgcaagga gtgtatgaag
      961 gtctcgtggt catgaactac gcggttttcg acgcgaaggc gaccgacgat ccagccggaa
     1021 cgcttgaatt ccatgcggcg cgcttcgggc ttggaaaggg aacaacgccg gagatgctca
     1081 aggg
//
LOCUS       DAOSWW010001054         1083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_131269_length_1083_cov_3.285992, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001054 DAOSWW010000000
VERSION     DAOSWW010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131269_length_1083_cov_3.285992"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PK442_15400"
     CDS             <1..239
                     /locus_tag="PK442_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89091.1"
                     /translation="EMLQTKISLTPSLAEFLDDHRSYGFKDKSLMVRSALLRFKEELE
                     LESLARSADLYAELYEQDEESRELTESALQGWPE"
     gene            236..589
                     /locus_tag="PK442_15405"
     CDS             236..589
                     /locus_tag="PK442_15405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOO89092.1"
                     /translation="MTLLKRGMVIDVNLSPTQGSETGKIRPCVVVTNDVYNAKVPVVQ
                     VVPVTEWNEKKGHIMTNVELLPSPENGLTKKSVADCLQTRPVDHRFRMVGVRGALTSE
                     EMHTIDDALRMVFSL"
     gene            complement(682..>1083)
                     /locus_tag="PK442_15410"
     CDS             complement(682..>1083)
                     /locus_tag="PK442_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89093.1"
                     /translation="VNLFKGIQDTWDERQALNKVAVKIPVREALVKSASAAGTDEQAI
                     VQYFHNPASDKRIVIFGHTHESRVIPSETHDGKSAVYVNSGTWIDKNNNLPTMTFVVI
                     APQGSSRHAGLYHYSHEGAITTLDTLKLEGF"
BASE COUNT          222 a          280 c          336 g          245 t
ORIGIN      
        1 ttgagatgct gcaaacaaaa atcagcctga caccgtcatt ggcggagttt ctggacgatc
       61 atcgctccta tggattcaaa gataaaagct tgatggtgcg cagcgcgctg cttcgtttca
      121 aagaagagct tgaactggaa agcctggcgc gttccgccga cctgtatgcg gagctctacg
      181 aacaggatga ggagtcccgc gagctgacgg agagcgcgct gcaggggtgg ccggaatgac
      241 cttgctgaag cggggcatgg tgatcgatgt gaatctttcc ccgacgcagg gatcggagac
      301 ggggaagatt cgcccgtgcg tcgtggtgac gaacgatgtg tacaatgcga aggtccctgt
      361 ggtgcaggtt gtgccggtca ccgagtggaa cgagaaaaaa gggcatatca tgacgaatgt
      421 cgagctgctt ccttcgcctg aaaacggcct gacgaaaaag tcggtggcgg actgtttgca
      481 gacccgtccc gtggatcacc ggttccgtat ggtcggggta cggggtgcgt tgacctccga
      541 agagatgcac acgatagacg acgcattgcg aatggttttt tcactctaat tcgctccgga
      601 catgcaaaaa tgagaaaaaa gagaggaagg cgcagagtcc gcgtcttcct ctcttcctgt
      661 tttccgcttc agccggtttc tttaaaagcc ttcgagcttc agagtatcca gcgtcgtgat
      721 ggcgccctcg tgcgagtagt ggtagaggcc tgcatgccgc gaggagccct gcggggcgat
      781 cacgacgaac gtcatcgtcg gcaagttgtt gttcttgtcg atccacgtgc cggagttcac
      841 gtacacggcg ctcttcccgt cgtgcgtctc cgaggggatc actctggatt cgtgggtgtg
      901 cccgaagatc acgatccgct tgtcggacgc ggggttgtgg aaatattgca cgatcgcctg
      961 ctcgtccgtt cccgccgcgg aggccgactt gacgagcgcc tctctgacgg gaattttgac
     1021 ggcgactttg ttgagagcct gccgttcgtc ccacgtgtcc tgtatccctt tgaacagatt
     1081 cac
//
LOCUS       DAOSWW010001055         1083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_131271_length_1083_cov_3.232490, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001055 DAOSWW010000000
VERSION     DAOSWW010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131271_length_1083_cov_3.232490"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PK442_15415"
     CDS             <1..948
                     /locus_tag="PK442_15415"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO89094.1"
                     /translation="DDLRSLHSPSASEVLVDVELSEDADEQPLWRYVLGFRSLENGML
                     AVSREEVARKGEMLLRRPGPDDIQDEALLSQTHLEQTQANARFREIAAFFSGIRYLHL
                     VPQLLKYPERAGVRSLDDDPFGAGFLEKLTGTDDSVRRARLEMIEAFLRTAVPGFDTL
                     QFVKDERGNPRLRMLHSHSGAAEGWRSEESFSDGTLRLIGLLWSLQEGGPVLLLEEPE
                     LSLNEGIAERLPEVMERLRNMDSKRSQLVICTQSEALLSNPGIDGRGVIALEVGAQGS
                     RGRVLNTQEMEALDAGFSISEAVLPRLRPKFLNRLGLKE"
BASE COUNT          211 a          280 c          349 g          243 t
ORIGIN      
        1 gatgacctgc gttctctcca ctcgccgtca gcttccgaag tcctggtgga tgtcgaactg
       61 tccgaggatg cggacgaaca gcctttgtgg aggtatgtcc tgggtttccg ttcattggag
      121 aacgggatgc ttgctgtttc ccgcgaagag gtggcgcgga agggcgagat gctgctgcgc
      181 cgtcccggtc ccgacgacat tcaggacgaa gcgctgctct ctcagacgca tctggagcag
      241 acgcaggcca atgcgcgttt ccgtgagatt gccgcattct tttccggcat tcgctatctt
      301 caccttgtgc cgcaactgct gaagtacccg gaaagggcag gggtacgctc tctggacgac
      361 gatcctttcg gcgcaggatt cctggaaaaa cttaccggca cggatgattc tgttcgccgc
      421 gcccgccttg agatgatcga agcattcctc cgaacggccg ttcccggatt cgatacgctg
      481 caattcgtca aggacgagag ggggaatccc cgcctgagaa tgctgcattc gcactctggg
      541 gcggcggaag ggtggcggag cgaggagtca ttttccgacg gtacgcttcg tcttatcggc
      601 ctcctttgga gtttgcagga gggcggcccc gtgttgttgc tggaggagcc cgaactctcg
      661 ctgaacgagg ggattgcgga aaggctgccg gaagtgatgg aacggcttcg aaacatggac
      721 tccaagcgtt cccagctcgt gatctgcacc cagagtgaag cgttgctttc gaatccgggg
      781 atcgatggtc gcggagtgat cgcgttggaa gtcggcgcac agggttcaag ggggcgtgtg
      841 ttgaatacac aggagatgga agcgctggac gcagggtttt caatatcgga ggccgttctc
      901 cccaggttgc gcccaaaatt cctgaatcgc ctcggattga aggaatgatg tattggaacg
      961 tggcaaccga ggatgaattg agcgaatccg ttgcggaacg tcttcttcgg tcgctttcgc
     1021 cgcctgtgga gctcgtcaac agattccgga gaagaggatt cggttatctt cggaagaatg
     1081 ccg
//
LOCUS       DAOSWW010001056         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_131484_length_1082_cov_3.131451, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001056 DAOSWW010000000
VERSION     DAOSWW010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131484_length_1082_cov_3.131451"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PK442_15420"
     CDS             complement(<1..449)
                     /locus_tag="PK442_15420"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOO89095.1"
                     /translation="MGEIYSSLYSIFHGTTLWAYAAVFAWGILGMILSPCSIATIPLV
                     VGYVGNTDAPDFKTAFKISLVFSLGVFVNLAFLGGLLASAGLFLGGIDQYTNYIVAGV
                     FVLFGLHLLGLISIPWFRGSSGPTTKHTGLKGALALGTLSGLALGPC"
     gene            complement(458..838)
                     /locus_tag="PK442_15425"
     CDS             complement(458..838)
                     /locus_tag="PK442_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOO89096.1"
                     /translation="MSLSRMFLFLTAIVVVFFLLPSVSNADEAPKKPTLVELMSPGCP
                     ACAEMEKVLAQFEEQYGDTVEVQIVNVREYPDVARLYQVRYVPTLVFIDENGKMLEKR
                     VGYMPLEALEKEWRERGYNIGKGE"
BASE COUNT          267 a          291 c          286 g          238 t
ORIGIN      
        1 ctgcacggcc ccagcgcaag ccccgagagc gtgccgagcg cgagcgctcc cttcagcccc
       61 gtatgcttgg tcgtcggccc ggatgacccg cgaaaccacg gaatggagat caggccgagc
      121 agatgcaatc cgaacagaac gaaaacgccc gcgacaatgt agttcgtata ttggtcgata
      181 ccgccgagga agaggcctgc cgaggcaagc aggcctccga ggaacgcaag gttgacaaaa
      241 acgcccagcg agaaaacgag cgatattttg aacgccgtct tgaagtcggg ggcgtctgtg
      301 ttccctacgt acccgacgac gagaggtatc gtggcgatgc tgcacgggct gaggatcatc
      361 cccaaaattc cccatgcgaa caccgccgcg tacgcccaca gagtcgtccc atgaaatatc
      421 gagtaaagag aggaatagat ttcgcccatg gataagctta ttccccttta ccgatattgt
      481 agccgcgttc cctccactcc ttttccagcg cttcaagcgg catgtatcct actcttttct
      541 ccagcatttt tccgttctcg tcgatgaaga ccagagtagg tacgtagcga acctgataga
      601 gacgggcaac atccgggtac tcccgcacat tgactatctg gacctcgacc gtatcgccgt
      661 actgctcctc gaattgggcc aaaacttttt ccatctcagc gcaggctggg cagcccggag
      721 acataagctc cacaagcgtg ggctttttgg gcgcttcgtc cgcgttggaa acggacggaa
      781 gcaggaaaaa tacgacaact atagctgtta aaaaaagaaa catccttgaa agactcatct
      841 ttaatccctc ccgggtgtat tttggtctgc taaacatact ggtattgtag tatttaaagt
      901 ggcggtaagt caagtgggtt tttcaatccc atccccattt ctccggtttt tcggtagaat
      961 gactgagaag gggtgtgaaa ctatgaaaaa gcatgaaatg actgcgatcg ttgaagcgat
     1021 ggaggaagag ctgatcgcgt tgcgtcgccg cttgcatacg tgtcccgagc tggattttgc
     1081 gc
//
LOCUS       DAOSWW010001057         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_131860_length_1080_cov_3.673171, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001057 DAOSWW010000000
VERSION     DAOSWW010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131860_length_1080_cov_3.673171"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..272
                     /locus_tag="PK442_15430"
     CDS             90..272
                     /locus_tag="PK442_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89097.1"
                     /translation="MPCNWAAGGIKEGEERGEKRGRERTALRMLEHGMDVALIAKLAE
                     LSEERVRRLAAQSSSQ"
     gene            complement(432..>1080)
                     /locus_tag="PK442_15435"
     CDS             complement(432..>1080)
                     /locus_tag="PK442_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89098.1"
                     /translation="FSEHAPTMSGAPDPMYFMNFKIIPDTIMGVRIYRGIEANILDFN
                     GAVDVDAAMASRLDYVIASLHPPIISSGTREENTRALVGAMRHPFVTIIGHPDDDRYP
                     LDYAELAAAAARERVALELNNSSFRPSTGRVNGRKNAVNLLNACRIHDARVVMNSDSH
                     IAFDVGDLSLCEEIIEETNFPRELVLNYTLEGLEYVLKEGVSLPDCEKLLCASGK"
BASE COUNT          192 a          307 c          367 g          214 t
ORIGIN      
        1 tggcgctcat cgccaagctt gccgaacttt cggaggagcg cgtccgtcgg ctcgctgcgc
       61 agtcctcgcc tcactgagga gaaatgagca tgccctgcaa ctgggccgcc ggcgggatca
      121 aggaaggcga agagagaggt gaaaagaggg ggcgcgagcg tacggcgctc cgcatgctgg
      181 agcacggaat ggatgtggcg ctcatcgcca agcttgccga actttcggag gagcgcgtcc
      241 gtcggctcgc cgcacaatcc tcgtctcaat gattgctcga acttcatcga gcgctccgtt
      301 gtctcaaacc agtaaaaagt gaaagccgct gttttttcaa caacgaaaac ggcggctttc
      361 ttcgattctt gaagcggagc ctgtcccccg acccgagatg agtcgtaccc gcttgcagcg
      421 aatgccccgc tttatttccc cgacgcgcag aggagctttt cgcagtcggg gagcgagacg
      481 ccctccttga gcacgtattc caggccttcc agtgtgtagt tcagcacgag ttcgcggggg
      541 aagttcgtct cttcgataat ctcctcgcag agcgacaggt cgccgacgtc gaaggcgatg
      601 tgggagtcgc tgttcatgac gacgcgggcg tcgtggatgc ggcaggcgtt cagcagattg
      661 accgcgttct tccgtccgtt cacccggccc gtgctcgggc ggaagctgga gttgttcagt
      721 tccagcgcga cacgctccct cgcggccgcg gcggcgagtt ccgcgtagtc gagcggatag
      781 cggtcgtcgt cggggtggcc gatgatggtc acgaaggggt gtcgcatcgc gccgacgagg
      841 gcgcgtgtgt tctcttccct cgtccccgac gagatgatgg gggggtgcag actggcgatg
      901 acgtagtcca gccgcgaggc catggccgcg tccacgtcga cggcgccgtt gaagtcgagg
      961 atgttggcct cgatgccacg gtagatgcgg acgcccatga tggtgtcggg gatgattttg
     1021 aagttcatga agtacatggg gtcgggcgcg cccgacatcg tgggggcgtg ctcggaaaag
//
LOCUS       DAOSWW010001058         1079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_132095_length_1079_cov_3.099609, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001058 DAOSWW010000000
VERSION     DAOSWW010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132095_length_1079_cov_3.099609"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PK442_15440"
     CDS             complement(<1..491)
                     /locus_tag="PK442_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO89099.1"
                     /translation="MVDTVLVLKTDVDTLRGYREGVPRLLEIFRARKMRASFFFSFGP
                     DNSGKAIRRIFKKGFLQKMRRTKAASAYGLRTLLYGTLLPAPKIVESEPSVFIRAVEE
                     GHDCGIHSWDHVHWHDNLSKMSEEQIRGEFRKAMELFERYARRTAKSCAAPGWQVTPA
                     SLAV"
     gene            complement(484..>1079)
                     /locus_tag="PK442_15445"
     CDS             complement(484..>1079)
                     /locus_tag="PK442_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOO89100.1"
                     /translation="IYSCGKQMMDRVIVAYGQEKGLRYTLFRPFNWIGPRLDTFKDAE
                     ERTARSITQMVYDVLSGRGITLVNGGEQMRSFTHISDGVAALMAILADEKASNGKIFN
                     IGNPANNASIKELAHIIINVMKEIPSFREAAEKTTVTVMPAEQYYGNGYDDMKNRVPS
                     IQAIGAALGWKPLVPLREAVAMTVASYLDSGEEQKRG"
BASE COUNT          175 a          333 c          337 g          234 t
ORIGIN      
        1 acggcgaggc tcgcgggcgt gacctgccag ccgggcgcgg cgcagctttt cgccgttctc
       61 cttgcgtacc gctcgaagag ctccatcgcc tttcgaaact ccccgcgtat ctgctcctcg
      121 ctcatttttg aaaggttgtc gtgccagtgg acgtggtccc agctgtgaat gccgcagtcg
      181 tgtccctcct cgacagcccg gatgaagacg ctcggctcgc tctccactat tttcggcgcc
      241 ggaagcagcg tgccgtagag cagggtgcgg agtccgtacg ccgaggcggc cttggtacgc
      301 cgcatcttct gcaggaaccc ttttttgaag atgcgccgga tcgccttgcc ggagttatcc
      361 ggaccgaagg agaagaagaa cgaggctcgc atcttcctgg cgcggaagat ctccaggagt
      421 cgggggacgc cctcgcggta gcctctcagc gtatcgacgt ccgtcttgag gacgagcacc
      481 gtatcaacca cgcttctgct cctctcctga atcaaggtag ctcgccacag tcatcgccac
      541 ggcctcgcgg agcggaacga gcggcttcca gccgagcgcg gcgccgatgg cctgaatcga
      601 aggaacgcgg ttcttcatgt cgtcgtagcc gttaccgtaa tactgttcgg cgggcatcac
      661 cgtcacggtg gtcttttccg cggcctcccg gaaagagggg atctctttca tcacgttgat
      721 gatgatgtgg gccaactcct tgatggatgc gttgttcgcc gggttgccga tgttgaagat
      781 cttcccgttg ctcgcttttt cgtcggcgag tatggccatc agcgccgcga cgccgtcgga
      841 gatgtgcgtg aagctccgca tctgctctcc gccgttgaca agggtgatcc cccgtccgga
      901 gagcacgtcg tagaccatct gcgtgatgga gcgggcggtg cgctcctcgg cgtccttgaa
      961 ggtgtcgagg cgggggccga tccagttgaa cgggcggaag agcgtgtagc gcaggccctt
     1021 ctcctggccg taggccacga tgaccctgtc catcatctgt ttaccgcagc tgtagatcc
//
LOCUS       DAOSWW010001059         1079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_132107_length_1079_cov_2.936523, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001059 DAOSWW010000000
VERSION     DAOSWW010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132107_length_1079_cov_2.936523"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..>1079)
                     /locus_tag="PK442_15450"
     CDS             complement(148..>1079)
                     /locus_tag="PK442_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOO89101.1"
                     /translation="DQLPGVTATKLLLNIEGPAAIDRLASLPADGVGLLRTEFIFSGE
                     IGTHPMHLVRTKQGSLFVDKLAEGMSTVARAFFPRPVLARFSDFRTNDFRKLNGGQEL
                     EPVESNPMIGWRGASRYISPDYEEGFRLECRAVKKVRDEMGNSNLSVIIPFVRTLKEM
                     ERVKAILASEGLASGASFKIWLMAEVPSVVFQADAFASLVDGITIGTNDLTQLILGVD
                     RDSAALENMGYFDERNPAVLHAVSLVIEAARRAGIPCSVCGEGPSVFPDFAEHLVREG
                     IDSISINADALEYTRRIVASVEQKIILDALRGK"
BASE COUNT          204 a          299 c          353 g          223 t
ORIGIN      
        1 tgtggaggac agataccagc agcttttgaa gcagtacggc ctgtccaact gagcagcctt
       61 ccctcaacct caacgagcaa agagcgccgg aactcccgca ttcctggggt ccgggcgctc
      121 tttttattgg gttgcgcgtc cctgacgtta cttgccgcga agggcgtcga ggataatctt
      181 ctgttcgacc gatgcgacta tgcggcgggt gtactccagt gcgtccgcat tgatgctgat
      241 gctgtcgatg ccttcccgga ccaggtgttc ggcgaagtcg ggaaagactg agggaccttc
      301 gccgcagacg gaacacggta ttccggcgcg gcgagcggct tcgatcacga gggaaacggc
      361 gtgaagcacc gcaggattgc gttcatcgaa gtaccccatg ttctccaggg ctgccgagtc
      421 tctgtcgacg ccgaggataa gctgcgtgag gtcgtttgtt ccgatggtga tgccgtcgac
      481 cagtgaggcg aaggcatctg cctggaagac aacagagggt acttccgcca tgagccagat
      541 cttgaacgaa gcgccggaag cgagcccttc ggaggcgagg atggccttga cccgctccat
      601 ctccttgagg gtgcggacaa aggggatgat gaccgagagg ttggagttgc ccatttcatc
      661 ccggactttt ttgacggccc tgcattcgag ccggaagccc tcttcatagt cgggggagat
      721 gtagcgggag gcgcctctcc atccgatcat cgggttggac tcgaccggtt cgagttcctg
      781 ccctccgttg agcttccgga agtcgttggt gcggaagtcg ctgaagcggg caaggacggg
      841 acgcgggaag aacgcgcgcg caaccgtgct catgccttcc gcgagtttgt cgacaaagag
      901 gctcccctgc tttgtgcgta cgaggtgcat cgggtgcgtt ccgatctcgc ctgaaaagat
      961 gaattccgta cggagaaggc cgacgccgtc cgccggcagt gaagccaagc ggtcgattgc
     1021 cgcagggccc tctatgttga gaagcagctt tgtcgcggtg actcccggga gctggtctg
//
LOCUS       DAOSWW010001060         1078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_132317_length_1078_cov_2.600196, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001060 DAOSWW010000000
VERSION     DAOSWW010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132317_length_1078_cov_2.600196"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK442_15455"
     CDS             complement(<1..618)
                     /locus_tag="PK442_15455"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOO89102.1"
                     /translation="MKKAMSVLLVAALVLGLAGGVVGASAADYPRKPITIVVPYSPGG
                     ASDTVSRIYAKELEKILNNPVIVTNRTGASGAVGLEFVKSSAPDGYTLAYMPVESTMI
                     SALGFTDTKSSDFTFIGRAMTIPASVTVRADSEWKTLDEFLEYAKQNPEKLRAGNSGT
                     GSIWHIAAALLERERGSKFIHVPFEGAAPAIAALMGKNIELVTVSP"
     gene            735..>1078
                     /locus_tag="PK442_15460"
     CDS             735..>1078
                     /locus_tag="PK442_15460"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO89103.1"
                     /translation="MALKKGNVHIVERDGFLAYSRQIRRLLKESDADILHAHFIHYKE
                     KLAALYACATCGHRVKTVLHLHNHLAIPKNPLRAIPQRLYLPFVARFVCCSRSVAAHL
                     IGDGVSQQRVCVA"
BASE COUNT          189 a          336 c          328 g          225 t
ORIGIN      
        1 gggggagacc gtgacaagtt cgatgttctt ccccatcagc gcggcgatcg cgggggcggc
       61 gccttcgaac ggcacgtgga tgaacttgga gccgcgctct ctctcaagaa gggctgcggc
      121 gatgtgccag atggagcccg tgcccgagtt tccggcgcgc agcttttccg ggttctgctt
      181 cgcgtactcg aggaactcgt cgagcgtctt ccactcggaa tcggctctca cggtgacgga
      241 ggcgggaatc gtcatggcgc ggccgatgaa ggtgaagtcg ctgctcttgg tgtcggtgaa
      301 gccaagcgcg ctgatcatcg tcgactcgac gggcatatag gcaagagtgt atccgtccgg
      361 ggcgctgctc ttgacgaatt cgaggccgac ggcgcccgaa gcgccggtgc ggttggtgac
      421 gatcacggga ttgttcagga tcttctcaag ctctttggcg tagattctgg agaccgtatc
      481 ggaagccccg ccgggcgaat acggaacaac gatggtgatc ggctttctcg gatagtccgc
      541 cgcggacgcg ccgacgactc cccctgcaag accaagaacc aacgccgcta caagcaacac
      601 gctcatcgct tttttcattt tgtctcctcc tgtcgttctc atgaacagct cttccactgc
      661 ccttgcgggc tccaaggtgc gcgccttcac agcgcgatgt tcgccgcgcc ggcgataccg
      721 cgtgggcccg cgaaatggcg ctgaaaaagg ggaacgtcca cattgtcgag cgggacggtt
      781 tccttgccta ttcccgccag attcgccggc ttttgaaaga gtccgacgcg gacattctgc
      841 atgcgcactt tatccattat aaggaaaagc tggccgcgct gtacgcttgc gccacctgcg
      901 gccaccgggt aaagacggtc ctgcacctgc ataaccacct cgcaattccg aaaaaccccc
      961 tgcgcgcgat ccctcagcgt ctgtatctgc ccttcgtggc acgcttcgtc tgctgcagcc
     1021 gttcggtcgc ggcgcatctg atcggcgacg gcgtttcgca gcagcgcgtc tgcgtcgc
//
LOCUS       DAOSWW010001061         1077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_132469_length_1077_cov_4.035225, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001061 DAOSWW010000000
VERSION     DAOSWW010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132469_length_1077_cov_4.035225"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(462..>1077)
                     /locus_tag="PK442_15465"
     CDS             complement(462..>1077)
                     /locus_tag="PK442_15465"
                     /inference="COORDINATES: protein motif:HMM:NF012968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOO89104.1"
                     /translation="NLMMTRPAFGGNIMATIVCAEHRPQMATVRPGVMQRIEPLKSSQ
                     PFVERMRVEGIAEGRNVEILEVVKKMSAKKDIQEARVLVAGGRGMGGPENFAVLEELA
                     EVLGGTVASSRASVDAGWVGKDRQVGQTGKTVRPDLYIACGISGAIQHLAGMEESGII
                     VAINKDPSAPIFEAADYGVVGDALKIVPMLAESIRNARARKAEV"
BASE COUNT          208 a          373 c          289 g          207 t
ORIGIN      
        1 caaagaggcg cacacgccgg tcgccacagc ctcctcaccc acatagagat gaacgaaccc
       61 cggcatcctc cccgccgcgt acagctccgc gcccctctcc tcgaacctcc tgatggtcag
      121 catcttccgg tacatcccga gcaacgtttc cttactgatc tccatacccg ttttccctcc
      181 ttgcacaaaa aaacttaatt tccaggcttc actgtaaaca cagccttatc ggaattatca
      241 tatacgctaa gacctgtcaa ggattttcac gggaggagaa gaaacgtata ctccgtacaa
      301 aacttttctt ttccatacgc agagcggccc tcttcacgag agtgaaaaga gccgccgggg
      361 atgaagcata attacgctct tcgcaagaaa gagacgggaa gcgtccgtgg aggagcgtcc
      421 ggaggtccga agcgcccgcg caacggagag actgccgcgc gctacacctc cgccttccgg
      481 gcgcgcgcgt tccggatgct ctcggcgagc atcgggacga tcttcagcgc atcgccgacc
      541 acgccgtagt cggccgcctc gaagatggga gccgaggggt ctttgttgat ggcgacgatg
      601 atcccggact cctccattcc ggcgaggtgc tggatggcgc cggagatgcc gcaggcgatg
      661 tagaggtccg gacggacggt cttccccgtc tgtcccacct gccggtcttt cccgacccag
      721 cccgcgtcga cgctcgcccg cgaggaggcg accgttccgc cgaggacctc cgccagctct
      781 tcgagaacgg cgaagttctc cggtccgccc atgccgcgcc ctccggcgac gagcacccgg
      841 gcctcctgta tgtccttttt cgcgctcatc ttcttcacga cttcgagaat ctccacattc
      901 cgcccctccg cgatcccttc gacgcgcatc cgttccacaa agggctgaga gctcttcagc
      961 ggctcgatcc tctgcatcac gccgggacgg acggtcgcca tctgcggacg gtgctccgcg
     1021 cagacgatgg tcgccataat gttgccgccg aaggccggac gcgtcatcat caggttc
//
LOCUS       DAOSWW010001062         1077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_132643_length_1077_cov_1.650685, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001062 DAOSWW010000000
VERSION     DAOSWW010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132643_length_1077_cov_1.650685"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1077
                     /locus_tag="PK442_15470"
     CDS             <1..>1077
                     /locus_tag="PK442_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89105.1"
                     /translation="KEESQSQFSARAGTLSISFEPLDVSTSSRPGKERAEAPKKDSPR
                     KEAASSVIAADGAFTPSRIVPLGSSGRNEAVEITAEEFALAESIGGVRAPSGRLFLVL
                     SLSWKNRLAPQEVLVPEGGSPHPAKWVRGGAEVKTVVMTPPYLVQDMKRHLFLRWGAE
                     ILSPSEGTWLLEDCLGVHGRWKLLVSPEADVEGKLVFLVSDKIPETAALDLYDTAYGM
                     ATVPLLGSPEATADRTASLARAPSGPMGAFTLRVEGTEDIRGKLGGGAIADGQLFRVL
                     RLGMTSEVQALLDFEPKKGTSLEFLSEKGTLRLSPSPVTEGLPGAFYGKVRLAPGSFN
                     TFRQAYVLPAQLADAPSRLRVELK"
BASE COUNT          167 a          375 c          354 g          181 t
ORIGIN      
        1 cgaaagagga atcgcagtcg cagttctccg cccgtgccgg aactctctcg atctcgttcg
       61 agccgctgga cgtctccacg tcgtcgcgtc cggggaagga gcgtgccgag gcgccgaaga
      121 aggattcccc ccgaaaagag gctgcgtcgt ctgtgatcgc cgctgacgga gccttcaccc
      181 cctcccgcat cgtcccgctc ggaagctcgg gccggaatga ggccgtcgag atcacggcag
      241 aggagttcgc gctcgccgaa agcatcggcg gcgtccgcgc cccttcgggg aggctgttcc
      301 tcgtcctctc gctctcgtgg aagaatcgcc tcgccccgca ggaggtcctc gtccccgagg
      361 gcggctcgcc gcacccggcg aagtgggtgc gcggcggcgc agaagtgaag accgtcgtca
      421 tgacgccccc ctacctggtg caggacatga agcgccatct cttcctccgg tggggcgcgg
      481 aaattctttc tccgtccgag gggacgtggc tgcttgagga ctgcctcggc gtccacggac
      541 gctggaagct gctcgtatcc ccggaggcgg atgtcgaagg gaagctggtc ttcctcgtct
      601 ccgacaaaat ccctgaaacg gccgcgctcg acctctacga caccgcctac ggcatggcta
      661 ccgtaccgct gctcggctct ccggaagcca ccgccgacag aacggcgtcg ttggcgcgcg
      721 cgccctccgg cccgatgggg gccttcacgc tccgcgtgga gggaacggag gacatccggg
      781 gaaagctcgg cggcggcgcg atcgccgacg ggcagctctt ccgggtgctt cgcctcggca
      841 tgacctcgga ggtgcaggcg ctgctcgact tcgagccaaa gaagggaacc tccctcgaat
      901 tcctctcgga gaaggggacg ctccgccttt caccgtcgcc ggtcaccgaa ggcctgccgg
      961 gcgcgttcta cggaaaggtc cgcctcgcgc cgggctcgtt caacacgttc cgtcaggcgt
     1021 acgtcctccc tgcgcagctt gcggacgctc cctcccgact tcgggtggag ctgaagg
//
LOCUS       DAOSWW010001063         1076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_132700_length_1076_cov_4.754163, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001063 DAOSWW010000000
VERSION     DAOSWW010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132700_length_1076_cov_4.754163"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PK442_15475"
     CDS             complement(<1..683)
                     /locus_tag="PK442_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89106.1"
                     /translation="MKRKVLFVFICIALSLFFVEVFGAWGVGGAFAEELTLIEQQKLA
                     RTLFNKIAAKDEQDVQGIEPLFLEVIEKAPRVKRAEEAYWMLSNMYMQAFDPPRNKDA
                     VALLEQYLTRYPRSRVLDDKFSMFTTPGLPLVKGRLLSLYEQEEMWEKAAALYDEVIP
                     EPKTAGEALLSHFIGYGRAMEQTGHKEKAIAAYTVFLERSGNTGDFLEEIVKDRLKEL
                     GVEVSSDTSV"
     gene            complement(765..>1076)
                     /locus_tag="PK442_15480"
     CDS             complement(765..>1076)
                     /locus_tag="PK442_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89107.1"
                     /translation="QVEAIKGIKIDKVTVWDSAGGASDKTSTANFLSGLMKSIPPLQD
                     MFKMAGMELPAYMKGGQKNEAPWPVPHEAGPERWMADAGEPVETDPHREMPPEVETEH
                     A"
BASE COUNT          189 a          347 c          298 g          242 t
ORIGIN      
        1 acggatgtgt ccgaggagac ttcgaccccc aactccttga ggcgatcctt cacgatctcc
       61 tcaaggaagt cccccgtatt tccgctgcgc tcaagaaata ccgtatacgc cgctatcgcc
      121 ttctctttgt gtccggtctg ctccatggcc ctgccgtacc cgatgaaatg gctcagcagc
      181 gcctcgccgg ctgtcttcgg ctccggaatg acttcgtcgt agagggccgc ggccttctcc
      241 cacatctcct cctgctcgta cagagagagg aggcgccctt tcacaagcgg gagtccgggg
      301 gtggtgaaca tggagaactt gtcgtccagt acgcgggagc gggggtagcg cgtcagatac
      361 tgctccagca gcgcgacggc gtctttgttt ctgggcggat cgaacgcctg catgtacatg
      421 ttgctgagca tccagtacgc ctcctcggcg cgcttcacac ggggagcttt ctcgataacc
      481 tccaggaaga gaggctcgat cccttgaaca tcctgttcgt ccttggcggc tatcttattg
      541 aagagcgtcc tcgcgagctt ctgctgctcg ataagcgtca gttcctccgc gaacgcgccc
      601 ccgacgcccc acgctccaaa gacctccacg aagaagagag agagcgcgat acagatgaac
      661 acaaacaaaa cctttctttt cactcggaac acctccccct ttgtcagagc atgcgcacaa
      721 ctacaggata cacacctgca ttccttccgc ggagcgttgg cgctctacgc gtgctccgtt
      781 tctacctccg gaggcatctc cctgtgcggg tcggtctcga cgggttcgcc cgcgtcggcc
      841 atccagcgtt cgggtccggc ctcgtgcgga acgggccagg gcgcttcgtt cttctgtccg
      901 cccttcatgt aggcgggcag ctccattccc gccattttga acatgtcctg gagcggaggg
      961 atggatttca tcaaaccgga gaggaagttc gcggtggatg tcttgtcgct ggctcctccg
     1021 gcgctgtccc agacggtgac cttgtcgatc ttgatcccct tgatggcctc gacctg
//
LOCUS       DAOSWW010001064         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_132933_length_1075_cov_3.147059, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001064 DAOSWW010000000
VERSION     DAOSWW010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132933_length_1075_cov_3.147059"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="PK442_15485"
     CDS             <1..168
                     /locus_tag="PK442_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOO89108.1"
                     /translation="QFTKALWDNIADLHKVHAKAKLITLETALDGVSVPVHPGAAKFY
                     TEKGMKVPAIK"
     gene            complement(225..>1075)
                     /locus_tag="PK442_15490"
     CDS             complement(225..>1075)
                     /locus_tag="PK442_15490"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89109.1"
                     /translation="LHVYKVAIGEEESTVFLTFSGEVCWDLLFAAEDEANKIVERNLP
                     VETLFFGREEAEQLPGIKGNWGRIADDTIRVVRIPGFDTIACSGSHVSATGEVGDIFV
                     TGFRGASPEWEVKFTAKGRKMRNEYSQVSRRMVRSVGCPLSRLEDVFSGLRDENAALG
                     KTLEKAAQLVAIPWDCSETGGVSLYTAVIPGVPKELVTAAARRWSDAHPGGIALLLLP
                     DPEKEGGAFLLYRGAAVERDFSRFLKSSPSLEARGGGRSDWLNGASSCMDPGAWTQEI
                     ERYMSE"
BASE COUNT          230 a          364 c          267 g          214 t
ORIGIN      
        1 cagtttacca aggccctctg ggacaacatc gccgacctcc acaaagttca tgcgaaggcg
       61 aagctgatca ccctcgaaac agcgctcgac ggcgtctccg tccccgtaca tccgggcgcg
      121 gcgaagttct acaccgagaa gggcatgaag gttcccgcta tcaagtagcg gcgcctttcc
      181 cagtttctca atgaaagaag gcggctctcc ggccgccttc ttttttattc cgacatatac
      241 cgttctattt cctgcgtcca cgctcccgga tccatacagg acgaagcgcc gttcagccag
      301 tcgcttcgcc cccctcctct ggcttcaaga gagggcgacg atttcaggaa gcgagaaaag
      361 tcacgctcta ccgcagcgcc tctgtagagg agaaacgctc ccccctcttt ctccgggtcg
      421 gggagcaaca gaagcgcgat ccccccgggg tgggcgtcgg accatctgcg cgccgcagcc
      481 gtcacgagct ccttcggaac tccgggaatg accgcggtat aaagggaaac tccgcccgtt
      541 tccgagcaat cccagggtat ggcaaccaac tgcgcagcct tctccagagt cttgccgagc
      601 gcggcgttct cgtcccgcag tcccgagaag acgtcctcaa gccgcgaaag cggacacccc
      661 acgctccgta ccatccggcg gctcacttgg ctgtactcgt tgcgcatctt tctccccttc
      721 gcggtaaact ttacttccca ttcgggggaa gctccccgga atcccgtaac gaagatatcc
      781 ccaacctcgc ccgtagcgga aacgtgcgac ccggaacagg caatggtatc aaaaccggga
      841 atgcggacaa cccgtatcgt gtcgtctgca attctccccc aatttccctt gatgccggga
      901 agctgttcag cctcctccct tccaaagaag agcgtttcca cgggaaggtt ccgttcgact
      961 atcttgttcg cttcatcctc cgcagcaaag agcaaatccc agcacacctc tccggagaag
     1021 gtgagaaaga ctgtactctc ctcttcaccg atagccacct tgtacacatg aagcg
//
LOCUS       DAOSWW010001065         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_133049_length_1075_cov_1.977451, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001065 DAOSWW010000000
VERSION     DAOSWW010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133049_length_1075_cov_1.977451"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..>1075)
                     /locus_tag="PK442_15495"
     CDS             complement(57..>1075)
                     /locus_tag="PK442_15495"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOO89110.1"
                     /translation="DTDEHVRIGLTMEQLAKRSGATGFFGSLAARLAGKTYALKRTES
                     PAPVAPTEKALFSALFAYNKNEILLRQENHSILGKARDRVKDQYKKSGKDLFSKNTLL
                     WLSGLLIPILGCLLLVLGQQEEIGVILGVATGTGTVAAFLLKHSVLTFKEDRGFFKKL
                     FKKFIPALFALLIAVSLFAVFGRGMIFIPLVALANAALIVVFNELMTIRTPKGNEVLA
                     EAEGLAMYMGTAEKRRLEMFNPPEETPEVFEALLPYAFALGVAETWANRFEDKLKEQQ
                     YKPTWYTGANLASFYTGSGIASVTSAVSSSIASAAVAPGSSSGSGGGGSSGGGGGGGG
                     GGGW"
BASE COUNT          205 a          318 c          332 g          220 t
ORIGIN      
        1 gcacacatgt cgcttttttg cattttcgaa cagtggtttc gtcggtcgaa acgcgcctac
       61 catccgccgc cgccgcctcc tcctcctcct cctccggaag agccgccgcc gccggagccc
      121 gacgaagagc ccggggcgac cgccgccgac gcgatggagc tggaaacggc cgaggtcacc
      181 gaggctatcc ccgaacccgt atagaaggac gccaggttcg cgcctgtgta ccacgtcggc
      241 ttgtactgct gctccttcag tttgtcctcg aagcggtttg cccacgtctc cgcgacgccg
      301 agcgcaaacg cataggggag gagggcttca aagacctcgg gggtctcctc gggaggattg
      361 aacatctcca ggcggcgctt ctcggcggtt cccatgtaca tggcaagtcc ttcagcctcc
      421 gcaagcactt cgttgccctt cggcgtccgg atcgtcatca gctcgttgaa aacgacgatg
      481 agtgcagcgt tcgccagcgc gacaaggggg atgaagatca ttccccgccc gaagacggcg
      541 aagagcgata ccgcgatgag cagcgcgaag agtgcgggga tgaatttttt gaagagcttc
      601 ttgaagaacc cgcggtcttc tttgaaagta agaaccgagt gtttgagaag gaatgcggcg
      661 acggtacccg tgccggtagc taccccgagg atgacgccga tctcctcctg ttgccccagg
      721 acgaggagca ggcagccgag tatcgggata aggagtcccg agagccagag cagcgtattt
      781 ttcgagaaga ggtccttccc gctttttttg tactgatctt tcacacggtc gcgcgccttg
      841 ccgagaatgc tgtggttctc ctgccggagc agaatctcgt ttttgttgta ggcgaaaagc
      901 gcggaaaaga gcgctttctc agttggggca acgggggccg gactctcggt gcgtttcaga
      961 gcgtatgtct ttcccgccag tcgggctgca agactgccga aaaagcccgt cgcgccgctg
     1021 cgcttggcga gctgttccat ggtcaggccg atgcggacgt gctcgtcggt atcga
//
LOCUS       DAOSWW010001066         1073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_133360_length_1073_cov_3.946955, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001066 DAOSWW010000000
VERSION     DAOSWW010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133360_length_1073_cov_3.946955"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK442_15500"
     CDS             <1..546
                     /locus_tag="PK442_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89111.1"
                     /translation="ELARSFQSDVLIHGKAQASTATKQKVSGVVLYAVNSQLQLKAII
                     TQTAHIIGAEGSEQRVQGTSPNDGAMKAFQKLSPRMAGSLLHTVAYALVSGASGSIPG
                     RSFKVTVSDLSFAEARSLRDSLGEVRGVNGVYQRAYANRTLELDVSAETSSEEIAIAL
                     EALGVEITGITAGTVNGRKGR"
     gene            550..1020
                     /locus_tag="PK442_15505"
     CDS             550..1020
                     /locus_tag="PK442_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89112.1"
                     /translation="MRVCRVLVCLLVVFFSSAAFASLPAEGTEDLAVLFQSIVESKSV
                     AEDIQVFRFGVVDWKGESVTSQGKAPLPSTSPQDQMLAKRGALTDARRNLLCLLYEIR
                     HGLPEKITSIEIEGDVVDGQVDFQGVKDGVYTVEVTVPLKRFFTESRIVRAGVR"
BASE COUNT          218 a          299 c          338 g          218 t
ORIGIN      
        1 gagcttgcac ggagttttca gtccgacgtg ctgatccacg gaaaggcgca ggcgagcaca
       61 gcgacgaaac agaaagtttc cggcgtcgtg ctctatgccg tcaactcgca gcttcagctc
      121 aaagcaatca tcacccagac ggcccatatc atcggcgccg agggctccga acagagagtg
      181 caggggacgt cgccgaacga cggcgcaatg aaggcgtttc aaaagctgtc accgcgcatg
      241 gccggctcgc tgctgcacac agtcgcctac gcgctcgtca gcggcgcttc cggcagtatc
      301 cccggaagat cgttcaaggt caccgtgagc gatctttcat tcgccgaggc caggtcgctt
      361 cgagacagtc tgggagaggt acgcggcgtc aacggagtct accagcgcgc ctacgcgaac
      421 cggacgctgg aactggatgt gtcggcggaa acctcttcgg aggagatcgc catcgcgctg
      481 gaggcgctcg gcgtggaaat caccggtatt accgcaggga cggtgaacgg acgaaagggg
      541 cgatagacgt tgagagtctg cagagtactt gtgtgccttc tggtcgtttt cttctcctcc
      601 gcagccttcg cgtcgcttcc tgcggaggga acggaggatt tggcggttct ctttcagtcc
      661 atcgtcgagt cgaagagcgt tgcggaagat attcaggtct ttcgcttcgg cgtcgtcgac
      721 tggaagggcg agtccgtgac gtcgcagggg aaggcccctc ttccttcaac ctccccgcag
      781 gaccagatgc tggcaaaacg gggggcgctg acggatgcgc gccgcaatct tctctgcctc
      841 ctttatgaga ttcgtcacgg acttccggag aagatcacct ccattgagat agagggcgat
      901 gtcgtcgacg gacaggtcga ttttcagggc gtgaaggacg gggtgtacac agtcgaggtg
      961 acggttccgc tgaagcgatt cttcaccgag agcagaatcg tccgcgccgg tgtgaggtaa
     1021 atgccatgaa aaaaatgctc gcagtctgca tggccgtcat ctttttcttt gcg
//
LOCUS       DAOSWW010001067         1069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_134263_length_1069_cov_3.344181, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001067 DAOSWW010000000
VERSION     DAOSWW010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134263_length_1069_cov_3.344181"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PK442_15510"
     CDS             complement(<1..1053)
                     /locus_tag="PK442_15510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO89113.1"
                     /translation="MSRYLDISVATLVGGLDMSTQVRSLKEGAVVVVGTPGRTLDHVN
                     RGSLKTENIHTIILDEGDHMLDMGFRDELEGILDALPHRNRTWLFSATMPREVKELSR
                     KYLSHPVSISLVEDGDQHEDIVHKAYLVPFRHKMEGLVNVLLWERPKRGLIFCHTRLE
                     TMNVSQRLNEEGFNSGSLHGEMTQRERNAVLSAFKNGHLPLLVATNVAARGLDVEGVT
                     HVIQIGLPDDRDTFVHRSGRTGRAGHEGMDILLLSPQEAEKFKYMIGSAKVNIEWNNV
                     PDVQAIGKVQREIAEEKLLTLKEASENEDYLQWAEDLLTRVNPKNLVARLLFSLTSKR
                     SAGYNLAADLERELRRK"
BASE COUNT          221 a          285 c          299 g          264 t
ORIGIN      
        1 ttttcttcga agctctcttt caagatcggc ggcgagattg tatccagcag agcgcttgga
       61 tgtcagtgaa aagagaagac gggcgacgag gtttttagga ttcacccttg tcagcaggtc
      121 ttcggcccac tgaaggtagt cttcattttc actggcttct ttcagcgtaa gcagtttttc
      181 ttccgctatc tctcgctgta ccttgccgat agcctgcacg tctgggacgt tgttccattc
      241 gatgttcacc ttggcggagc cgatcatgta cttgaacttt tccgcttcct gcggagagag
      301 aagaaggata tccatacctt cgtgtcctgc tctgcctgta cgtccgctgc ggtggacgaa
      361 ggtatccctg tcgtcgggaa ggccgatctg tatgacgtgg gtcactccct cgacgtccag
      421 gccgcgcgcc gctacatttg ttgcgaccag aaggggaaga tggccgttct tgaaggctga
      481 aaggacggcg ttccgctctc tctgggtcat ctctccatgg agcgagccgg aattgaagcc
      541 ctcctcgtta agacgctgag agacgttcat cgtttcaaga cgggtatggc agaagatcag
      601 tccccgcttc gggcgctccc aaagaagaac gttcaccaat ccctccatct tatgccggaa
      661 tggaaccagg taggccttgt ggacaatatc ctcatgctgg tcgccgtctt ctaccaaaga
      721 aatggatacg gggtgggaaa ggtacttccg agagagttcc ttgacctctc tcggcatagt
      781 cgctgagaag agccacgtcc tgttgcgatg gggaagcgcg tcgagaatgc cttcaagttc
      841 gtcgcggaat cccatgtcga gcatgtgatc accctcgtcg aggatgatgg tgtgaatatt
      901 ttccgttttc agcgaaccgc ggttgacatg atcaagcgtt cttccggggg ttccaacgac
      961 gacgacggcg ccttctttca gtgaacgaac ctgagtcgac atgtcaagcc cgccaacgag
     1021 agtggcgacg gagatatcga ggtagcgtga cagccattcc gcctcacgc
//
LOCUS       DAOSWW010001068         1068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_134447_length_1068_cov_3.690030, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001068 DAOSWW010000000
VERSION     DAOSWW010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134447_length_1068_cov_3.690030"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PK442_15515"
     CDS             complement(<1..1046)
                     /locus_tag="PK442_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89114.1"
                     /translation="MCRLFGNVDIDVARRLKELFPPTVETLPDIAELMRECRCDAATF
                     LVIDNYQLFESPIPYHIAHAFSAHSNPNLHMLFITQPLPTFEIAVQDANIHRLGTRDF
                     FFDRENTARLCRTAGARMTEKEIDRIQSVSEGWVSAILLQASNYMETGAFADARDMDA
                     LVETAIWNRMTGEEHEFLLGLSLLDAFSPRQAAIMAGGPTLPEHLLKMLKNGFFISYI
                     ADKGVYSLHSILNDYLKQRFENQPREFAEIMIRRAGAACAAASDYFPAAQFFSRVRDY
                     GAILSMPLTTSYLNEQKEKDIVGFLERFVGECPEQTLQEYPFALLTFAFQFMKSGKRA
                     LFSQMVRLLRSITDA"
BASE COUNT          232 a          283 c          313 g          240 t
ORIGIN      
        1 gcgtccgtga tagagcgcaa caggcggacc atctgcgaaa aaagcgccct tttgccgctc
       61 ttcatgaact gaaacgcgaa ggtgaggagg gcgaacggat attcttgaag agtctgttcc
      121 gggcattctc cgacgaatcg ttcaagaaag ccgacgatgt ctttttcctt ctgttcgttc
      181 aaataacttg tcgtgagcgg cattgagaga atggcgccgt agtcccgaac cctggagaaa
      241 aactgcgcgg ccggaaagta gtccgaagcg gcggcgcagg ctgcgcccgc cctgcgaatc
      301 atgatctccg caaattcgcg gggttggttc tcgaagcgct gtttcagata gtcgttcagg
      361 atgctgtgga gagaatagac gcctttgtcc gcgatgtacg agataaaaaa accgtttttc
      421 agcatcttga gcaggtgttc cggcaaggtc gggccccctg ccatgatagc cgcctgtctg
      481 ggggagaaag cgtcgaggag agagagccca aggaggaatt catgttcttc cccggtcatt
      541 ctgttccata tcgccgtttc gacgagcgcg tccatgtcgc gcgcgtcggc gaacgccccc
      601 gtttccatgt agttcgaggc ctggagcagg atggcggaga cccatccttc ggagacgctc
      661 tgaatccggt cgatttcctt ctccgtcatg cgtgctccgg ccgtacggca gagtcgcgcc
      721 gtattctcgc ggtcgaagaa gaaatctctc gttcccagtc tgtgtatgtt tgcgtcctga
      781 accgcgattt caaaagtcgg aagaggctgg gttatgaaga gcatgtggag attcgggttt
      841 gaatgcgcgg aaaaggcgtg cgcaatatgg tacgggatcg gactttcaaa cagttgatag
      901 ttgtcgatca caagaaacgt cgcggcatcg catcggcact cgcgcatcag ctccgcgata
      961 tcgggaagcg tttcgaccgt tggggggaac aactccttca gtcttcgcgc aacgtcgata
     1021 tccacattcc cgaacagtct gcacacacct tcccacacct tcgagggg
//
LOCUS       DAOSWW010001069         1068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_134484_length_1068_cov_2.985192, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001069 DAOSWW010000000
VERSION     DAOSWW010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134484_length_1068_cov_2.985192"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK442_15520"
     CDS             <1..695
                     /locus_tag="PK442_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOO89115.1"
                     /translation="FAVNIAQFGGQGAKRPVLIFSLEMGAEQLVQRMLGAEARINIHD
                     LRNGSFPKSAWESLAEAAGRLAEAPIYIDDSSMLSTLEFRARCRRFKSKHKDLGLVLV
                     DYLQLMNSSRRIDNKQQEVAEISRALKGVARELEAPVIALSQLSRAVEQRNEKKPQLS
                     DLRDSGAIEQDADTVILLYRPGYYDAGAPGEEEDNRAFISLAKHRNGPTGEISLIFLR
                     EYTRFVNADRRY"
     gene            705..>1068
                     /locus_tag="PK442_15525"
     CDS             705..>1068
                     /locus_tag="PK442_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOO89116.1"
                     /translation="MLEENAFYLYNSSEKLETWGAVFLTPAVADGIRTRGYSAPERSS
                     KIPGENDGMAGFLNFLRGDIAMNDGNEAAFSDTKAERRFADIPVDLIRPNPAQPRQFF
                     DEAELEELAASIAEVGVIQ"
BASE COUNT          255 a          274 c          314 g          225 t
ORIGIN      
        1 catttgcggt caacattgcg cagttcgggg gacagggagc aaagcgccct gtccttatct
       61 tcagcctcga aatgggcgcc gaacagcttg tccagcgaat gctcggagcg gaggcgagaa
      121 tcaacattca cgatctcagg aacggctctt ttccgaaatc ggcatgggag tcgctcgccg
      181 aggccgcagg gagacttgct gaggctccta tttatatcga tgacagttca atgctctcga
      241 cgctcgaatt ccgcgcccgg tgcaggcgtt tcaagtcgaa gcacaaggat ctcgggcttg
      301 tcctggtaga ctaccttcag ctgatgaatt cctccaggag gatagacaat aaacagcagg
      361 aagtcgcgga aatttccagg gcgctcaagg gcgtggcccg tgaactggaa gctccggtta
      421 tcgcgctctc gcagctctcg cgtgctgtgg aacagcggaa cgagaaaaaa ccgcagcttt
      481 ccgatctgcg tgacagcggc gcaatcgaac aggatgcgga tactgtcata cttctctacc
      541 gccccgggta ctatgatgca ggagccccgg gggaagaaga ggacaaccgt gcatttatca
      601 gtctcgccaa gcatagaaac gggccgaccg gcgagatctc actgatcttt ctccgggagt
      661 atacccgttt tgtcaatgcg gaccgccgtt attaagcgtt ccgtgtgctt gaagagaacg
      721 cgttctatct gtacaatagt tcggagaagt tagaaacatg gggcgccgtc ttcttgactc
      781 cggccgtcgc tgacggaata cggacgcggg gatactccgc gccggaacgg agctccaaaa
      841 taccggggga gaatgatggg atggctggat ttttgaactt tttgagaggt gacattgcaa
      901 tgaacgacgg aaacgaggcg gcgttctccg ataccaaagc ggaaagacga ttcgccgata
      961 ttccggtcga tctcattcga ccgaatccag cgcaacccag acagtttttt gacgaggcgg
     1021 agctggaaga acttgctgct tcaatagcag aggtgggcgt tatacagc
//
LOCUS       DAOSWW010001070         1063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_135598_length_1063_cov_2.405754, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001070 DAOSWW010000000
VERSION     DAOSWW010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135598_length_1063_cov_2.405754"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1063
                     /locus_tag="PK442_15530"
     CDS             <1..>1063
                     /locus_tag="PK442_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89117.1"
                     /translation="VSALPRLFPVAGGLPEALAPGFAHNRPVDVLAESYASVQGVDAA
                     AAFFSEAALSGQGGGVHRDSNDVYWFQSMRSGLASLFRIAGLLACRKGETRSLNRILC
                     DCFDDLYDAVSRTSPNGTPERPKWLEIIPEEVRRPIVLNYLGKAGPSTYSHLSTLAVA
                     VGELRRGAPESKTAPFAAMNDGAPLFIYAPAWGNAALNILLRICMRGNYALLLPEVHL
                     WPEAELLRIGHALRYGASGCSVVWTSLSVPAHPAFDSDWEIFGKSSSSDVLGLFRARL
                     NRYGAEGESRLKDLKYETPASLDDLHALQRTPSGWSIVPCTPFDERETVELEGEGPPL
                     SKEEIFLANMAELAVFAPET"
BASE COUNT          192 a          345 c          332 g          194 t
ORIGIN      
        1 gtcagcgcgc tgcccaggct cttccctgtg gcgggggggc tgcccgaggc gctcgcgccc
       61 gggtttgcgc acaaccgccc cgtcgacgtc cttgccgaga gctacgcttc cgtgcagggt
      121 gtagacgctg cggccgcatt cttctccgaa gccgctctct ccggccaagg gggaggagtg
      181 caccgcgatt ccaacgacgt ctactggttc cagagcatgc gctccgggct ggcgagcctc
      241 ttccggatcg cggggctgct cgcgtgcaga aaaggagaaa cccgttcgct caacagaatc
      301 ctgtgcgact gcttcgacga cctgtacgac gcggtcagcc ggacgtcgcc caacggaact
      361 cccgaacgtc ctaagtggct ggaaatcata ccggaagaag tgcgcaggcc gattgtgctg
      421 aactacctgg ggaaggccgg cccttccacc tattcgcacc tttcgacgct cgccgtagcg
      481 gtgggggaac tccgcagagg ggccccggaa agtaaaacag cgccgtttgc cgcaatgaac
      541 gacggggcgc cgctctttat ctacgctccc gcctggggaa acgctgcgct caacatcctg
      601 cttcgtatct gcatgcgggg taactatgca cttcttctcc ctgaggtgca cctctggccc
      661 gaggcggagc tgcttcgcat cgggcacgcc ctcaggtacg gggcgagcgg atgcagcgtc
      721 gtctggacgt ctctctccgt acctgcccac cccgccttcg acagcgactg ggagatcttc
      781 gggaagagtt cgtcctccga cgtgctgggg ctcttccggg cgcggctcaa cagatacgga
      841 gcggaagggg agagtcgcct caaggatctg aaatacgaaa cgccggcgtc gttggacgac
      901 cttcacgcgc ttcagcgcac tccctccgga tggagcatcg ttccgtgcac ccccttcgac
      961 gaacgggaaa cagtcgaact ggagggggaa gggcccccgc tctcaaagga agagattttt
     1021 ctggcgaaca tggccgaact cgcggtattt gctccggaaa ccc
//
LOCUS       DAOSWW010001071         1062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_135747_length_1062_cov_4.318769, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001071 DAOSWW010000000
VERSION     DAOSWW010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135747_length_1062_cov_4.318769"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1062
                     /locus_tag="PK442_15535"
     CDS             107..>1062
                     /locus_tag="PK442_15535"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOO89118.1"
                     /translation="MNDILQRIGELGIVPVVKLDKASDALPLGKALLEGELPIAEVTF
                     RTDAAEESIRIMSRELPELLVGAGTVLTRDQVKKAVDAGAKFIVTPGFNPSVVDYCVE
                     HNIPITPGINSPSQIEMALERGLDVVKFFPAEQSGGLPMLKAMSAPYGSVRFIPTGGV
                     DAKNMPSYLAFNKILAVGGSWMVKPEVVAEGKFDEIARMTREAVFAMHGFELIHLGIN
                     CPDEKEALAGAERLAALFGMAVKDGNSSAFVGKGFEFMKSMYLGEKGHVAVGCNDVAR
                     AAAFLKRKGIGSLPETEKKKDGRTVALYLDLHIGGFIFHLLQ"
BASE COUNT          220 a          324 c          334 g          184 t
ORIGIN      
        1 atttcgaaac aatgttttat tctgcggaaa aaagtccttg acgaaagggc gattcattgt
       61 ctatgcttga cgcgtcagcc acataggaaa ggagacgaaa aataatatga acgacatact
      121 gcagcgcata ggggaactcg gcatcgttcc cgtggtcaaa ctcgacaagg cctccgacgc
      181 tctccctctc ggcaaggcgc ttcttgaagg ggaactcccc atcgcggagg tgaccttccg
      241 taccgacgcg gcggaggagt ccatccgcat catgtcgcgc gagcttccgg agctgctggt
      301 gggcgccggg accgtgctca cccgcgacca ggtgaagaag gccgtggacg cgggggcgaa
      361 gttcatcgtt acgccggggt tcaacccctc ggtcgtggac tactgcgtcg agcacaacat
      421 ccccataacc cccgggatca acagcccgtc gcagatcgag atggcgctgg agcgcgggct
      481 cgacgtggtg aagttcttcc cggccgagca gtcgggcggt ctgcccatgc tcaaggcgat
      541 gagcgctccg tacggaagcg tccgcttcat ccccaccggc ggcgtcgacg cgaagaacat
      601 gccctcctac ctcgccttca acaagatcct cgccgtgggc gggagctgga tggtgaagcc
      661 cgaggtcgtc gcggagggga agttcgacga gatcgcccgc atgaccagag aagccgtctt
      721 cgcgatgcac ggcttcgagc tgatccacct gggaatcaac tgtcccgatg aaaaagaggc
      781 gcttgccggc gccgagaggc tcgccgccct cttcggcatg gcggtgaagg acgggaacag
      841 ctccgccttc gtcgggaagg gcttcgagtt catgaagagc atgtacctcg gcgagaaggg
      901 gcacgtcgcc gtcggctgca acgacgtcgc gcgcgccgcc gccttcctga agcggaaggg
      961 gataggttcc ctcccggaga ccgagaagaa gaaggacggc cgcacggtcg cactctacct
     1021 cgacctgcac atcggcggct tcatcttcca tctgttgcag aa
//
LOCUS       DAOSWW010001072         1062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_135761_length_1062_cov_3.427011, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001072 DAOSWW010000000
VERSION     DAOSWW010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135761_length_1062_cov_3.427011"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1062
                     /gene="htpG"
                     /locus_tag="PK442_15540"
     CDS             <1..>1062
                     /gene="htpG"
                     /locus_tag="PK442_15540"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOO89119.1"
                     /translation="MNQDELKSYIGTIAKSGAREYLAAIEQKGESLQAETLIGQFGVG
                     FYSAFMVADKVDVVTRRLGEHAAWRFSSTGDGAYTIEQAERAEPGTTVFLHLKTPDVA
                     RGEKDYSEEWTIREIVKKYSDFITYPILMEVVRKKDDKDETSDERLNSGKAIWRRPEK
                     EVSEEEYAEFYKHSSRDWNDPLTRLVFIVEGGAEFRGLLFIPSKAPYDLMFAPRREGV
                     SLYIRNVFIMNDCKELIPSWLRFLRGVVDSEDLPLNISRELLQEDPLLRVIRKSVVRR
                     VLNALKKMLNESREKYENFWREFGQVIKEGLVSLDGREEENREAILDICLFHTTAGDT
                     MSTLKEYVDALPEGQEHIY"
BASE COUNT          275 a          248 c          310 g          229 t
ORIGIN      
        1 tatgaatcag gacgagctga aatcgtatat cggcacaata gcaaaatcag gcgcccgcga
       61 gtaccttgca gcgatagaac aaaaagggga gtcattgcag gccgaaacgc tcatcggaca
      121 gttcggggtc ggtttctatt ccgcgttcat ggtcgcagac aaagttgacg ttgtcacaag
      181 gcgtcttgga gaacatgcgg cctggcgttt ttcttctact ggagacgggg cctacactat
      241 tgagcaggct gaaagggcgg aaccggggac aacggtcttt ctgcatctca agactccgga
      301 tgttgcgcgc ggggaaaaag actactcgga agaatggact atccgtgaaa tagtaaaaaa
      361 gtactccgac ttcatcacgt acccgattct catggaggtc gtcaggaaga aggacgacaa
      421 agacgagacc tccgacgagc ggttgaactc gggaaaggct atttggcggc gtccggagaa
      481 agaggtttcg gaagaagagt acgcggaatt ctacaagcat tcgagcagag actggaacga
      541 tccgctgacc cggctggttt tcattgtgga gggcggcgcc gagttcaggg gacttctttt
      601 tattccttcg aaagcgccct acgaccttat gttcgcccct cgaagagaag gcgtctctct
      661 gtatatacgg aatgtgttta tcatgaacga ctgcaaagag ctgattccct cttggcttcg
      721 cttcttgcgg ggcgtggtcg actccgagga cttgccgctg aacatctccc gcgaactcct
      781 gcaggaggat ccgctgttgc gggttatcag aaaaagcgtt gtccgccggg tgctcaacgc
      841 gctgaaaaaa atgctgaacg aaagccgaga gaagtacgag aacttctgga gagagttcgg
      901 acaggtaata aaagaagggc tcgtctcgct tgacggtcgc gaagaggaga accgcgaggc
      961 tattcttgat atctgtcttt tccatacgac agccggagac acaatgagta cgttgaagga
     1021 gtatgtcgat gctctacccg agggacagga gcacatctac ta
//
LOCUS       DAOSWW010001073         1062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_135803_length_1062_cov_2.503476, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001073 DAOSWW010000000
VERSION     DAOSWW010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135803_length_1062_cov_2.503476"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PK442_15545"
     CDS             <1..683
                     /locus_tag="PK442_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89120.1"
                     /translation="ALLDMECAKAELEDVRTEIWSKTEGVFEVRPTRRGALALKASFL
                     SVVLLFSLAAPLAIQSSRPASSVYQASGNVHSGEHVLEWVTGDEQAVLAALRKSLSDA
                     NRAWSAAALRDETDAVAGEAASGPAMNEESLEEEKTYVARAPSASEGSGASRESGAET
                     VSRRVRPKAQGELAAGSAGLKNEVVAGEDAIRPSSVANELDSIISLVQIGQKALRERE
                     SPIQFQRP"
     gene            814..>1062
                     /locus_tag="PK442_15550"
     CDS             814..>1062
                     /locus_tag="PK442_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOO89121.1"
                     /translation="MKGKIGRSWPAACIFFLMAAILFGANPAAAADPLVAALDVEGND
                     HVVSEHILGVIRTAVGEPLSREQLQSDVEAIYALGFFSF"
BASE COUNT          210 a          264 c          335 g          253 t
ORIGIN      
        1 gcgcgctgct tgatatggag tgcgcgaaag ccgagctgga agatgtccgg acggaaatct
       61 ggtcgaagac cgagggggtt ttcgaggtgc ggccgaccag gagaggcgct ctcgccctga
      121 aggcgtcttt tttgagcgtc gtccttcttt tttctcttgc cgcgccgctc gcgatacaga
      181 gttcgagacc ggcgtcttct gtgtatcagg cgagcggaaa tgttcactcg ggagagcatg
      241 tccttgaatg ggtgactggc gacgagcagg ctgttcttgc cgctcttcgt aaaagcctga
      301 gcgacgcgaa tcgagcatgg agcgcggcgg cgcttcgaga tgaaaccgat gcggttgctg
      361 gtgaagcggc gagcggcccc gccatgaacg aagaatcgct tgaggaggag aaaacctatg
      421 tcgcccgcgc tccgtctgcg tcggaaggaa gcggagcttc ccgtgagtcc ggagccgaaa
      481 ctgtttcccg ccgggtgcgt ccgaaagctc agggagaact tgcggcaggc tcagctggac
      541 tgaagaacga agtcgtcgcc ggtgaagacg ctatccgccc ttcctcagtg gcaaatgaac
      601 tcgattcaat aatctccctt gtgcagatag gacaaaaagc cttgcgcgag agggagtctc
      661 cgatacagtt tcaacgtccg tgacgcaaac ggccggttgt ttttttcgga cttctgattt
      721 tgctttgttg atggcgtgcg ttttactgag tgtatatttt ttcgtcccgt tcaggacgaa
      781 ttcatttttg ttttttggaa ggagagagcg tgcgtgaaag gaaaaattgg aaggtcctgg
      841 ccggcagcgt gtatcttttt tttaatggcg gcgattctgt tcggagcgaa tcccgccgca
      901 gcagctgacc ctctggtcgc agcgcttgat gtggagggga acgaccatgt cgtctccgaa
      961 cacattcttg gagtgatccg aacggccgta ggagagccgc tcagccgtga gcagctccag
     1021 agcgacgttg aggccatcta tgccttgggt ttcttctcgt tc
//
LOCUS       DAOSWW010001074         1059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_136393_length_1059_cov_4.719124, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001074 DAOSWW010000000
VERSION     DAOSWW010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136393_length_1059_cov_4.719124"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PK442_15555"
     CDS             <1..653
                     /locus_tag="PK442_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="necessary for biosynthesis of osmoregulated
                     periplasmic glucans possibly involved in the transfer to
                     the periplasmic space; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HOO89122.1"
                     /translation="HSVFILSTILGKNVGWPTQSRDDRGTPWADAVDVHWWGTILGIL
                     WGALVYLTNPSFFWWISPIILSLVLSIPLSVLTSRASLGKAFLRAGFFTTPEETEPPV
                     ELRELAKTLQEEAPYSPLPLSPRQGFLRAVTDPCVHALHTSLLLRYKKGAPAREEARQ
                     ALVEKALRSGPDSLSSKEKMTLLNDPFHLAELHRRVWELEDDKQAARWGLSQPSVD"
     gene            868..>1059
                     /locus_tag="PK442_15560"
     CDS             868..>1059
                     /locus_tag="PK442_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89123.1"
                     /translation="MMTGLPKNIPANTSPERVAWRTSRDRVLLYLRGLDLPPFEDLDL
                     AAASLRACGTREESAAEGPG"
BASE COUNT          201 a          352 c          304 g          202 t
ORIGIN      
        1 atcacagcgt cttcatcctc tcgaccattc tgggcaaaaa cgtcggttgg ccgacccaat
       61 cccgcgacga ccggggaacc ccatgggcgg acgccgtcga cgttcactgg tgggggacga
      121 tactcggcat cctctggggc gcgctcgtct acctgacgaa cccctccttc ttctggtgga
      181 tatcgcccat catcctctcg ctcgtactct ccatcccgct ctccgtcctc acgagcaggg
      241 cgagcctggg gaaggcgttc ctgcgggcgg ggttcttcac aactccggag gagacggaac
      301 cgccggtgga gctgcgcgag ctggcgaaaa cccttcaaga ggaagcgccg tactcacctc
      361 ttcctctgtc accacggcag ggattcctcc gcgccgtgac cgacccgtgc gtccatgcgc
      421 tccatacgtc gctgctgctg cgctacaaaa agggagcgcc cgcgcgggaa gaggcgcggc
      481 aggccctcgt ggagaaagcg cttcgttccg gtcccgactc tctttcctca aaggagaaaa
      541 tgacgctcct gaacgatccc tttcacctcg ccgagcttca caggcgagtc tgggagctgg
      601 aggacgacaa acaggccgcc cggtgggggc tctcgcagcc gtccgtcgac tgaacacgca
      661 cgtgagagga gcggaatttg ctcctctcac tgttttttac cttctctctt caaccaaaag
      721 gaagtatact caataaatac acccacacga tcaaggagag gattttatgc actgcaccgg
      781 atactccgcc tgcacaccgc cgcgttctcc tgcctccttc gcgctcgcgg ggattctttc
      841 aacaaaaggc tttgcggagg aggatgaatg atgacgggac tcccgaagaa cattccggcg
      901 aatacgtctc cggagcgcgt tgcgtggcga acctcgcgcg accgcgtgct cctctatctg
      961 cgcggactgg atctgccgcc gttcgaagat ctcgacctgg ccgccgcaag cctccgcgcc
     1021 tgcggaacgc gtgaggaatc cgctgcggaa gggccgggc
//
LOCUS       DAOSWW010001075         1059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_136528_length_1059_cov_1.974104, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001075 DAOSWW010000000
VERSION     DAOSWW010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136528_length_1059_cov_1.974104"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK442_15565"
     CDS             complement(<1..198)
                     /locus_tag="PK442_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89124.1"
                     /translation="MPVPLDTSRALEILRQLIRIRTVQPYGDEKDAVGYILSLFPEEC
                     IEKIVVDHGDNRASLVLVVPGE"
     gene            complement(269..>1059)
                     /locus_tag="PK442_15570"
     CDS             complement(269..>1059)
                     /locus_tag="PK442_15570"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO89125.1"
                     /translation="EAEESFAQWGWKKDQEFLVLVLRLADDVKEDDGFSARERHISFS
                     LARSKVLPFFPKSFCVFYTKMPVFLLPLPSGKPEALSGVKDVLESILLSLKDELPPKK
                     GRAFLFAAGGAKRGVNAIHRSFDEAVRVLSVSESLRREGGVILWDMLGGYQLVAILSE
                     TEEARLFCGRILGELLEGTDSSSVELLETLLVMDGCNWNVRAASESLRFHYNTVKYRL
                     GVLRERIGFDSSDPAQRFDMGLALRLAPLFFKDKFVTICQKPFI"
BASE COUNT          278 a          306 c          252 g          223 t
ORIGIN      
        1 ctcccccggc acaacaagca ccagggatgc acggttatct ccgtgatcga ccactatttt
       61 ttcaatacat tcttcgggaa agagagagag gatgtacccg acggcatcct tttcatcgcc
      121 gtagggctgc actgtccgaa tacggatgag ctgacgcagt atttccaggg cccgcgaggt
      181 atccagagga acaggcattc tatcccctcc ttgagtatct caaggatacg gggaaacacc
      241 ttttttttca ttatccggtt cggccaaatc agataaaagg tttttggcaa atagtaacaa
      301 atttatcttt aaaaaataac ggcgcaaggc gaagggcaag ccccatatca aagcgttgcg
      361 ccgggtcgga agagtcaaat ccaatccgct cccgcagaac accaaggcgg tacttcactg
      421 tattataatg aaaacggagc gactcggacg ccgccctgac gttccagttg cacccgtcca
      481 tcaccagcaa ggtctccaga agttccactg aggaagaatc ggttccctcc agcagttcgc
      541 ccaggatccg gccgcaaaaa aggcgcgcct cctcggtttc ggagaggatc gcaaccagct
      601 ggtatccgcc gagcatatcc caaagaatga cgcccccttc tctccggagg ctttctgaga
      661 ctgaaagcac tcgtaccgct tcgtcgaagc tccggtgaat tgcgttcaca cctctcttgg
      721 cgcctccggc cgcaaagaga aaagcgcgtc ctttcttcgg cggaagctcg tctttcagcg
      781 aaaggagtat gctctcaaga acgtctttca ctccggaaag ggcctccggt tttccggagg
      841 ggagcggaag gagaaacacc ggcatctttg tataaaaaac acaaaaagac ttggggaaaa
      901 aagggagcac cttgcttcgt gcaagtgaga aggaaatatg acgctccctc gccgaaaacc
      961 catcatcttc ctttacatcg tccgcaagac gaagaaccag cacaagaaac tcctggtcct
     1021 ttttccaccc ccattgcgca aacgactcct ccgcttccc
//
LOCUS       DAOSWW010001076         1058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_136659_length_1058_cov_2.750748, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001076 DAOSWW010000000
VERSION     DAOSWW010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136659_length_1058_cov_2.750748"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PK442_15575"
     CDS             complement(<1..396)
                     /locus_tag="PK442_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOO89126.1"
                     /translation="MKSTDELMDEHRGIEVMLDVLDAVARKVATGKTADLGHLDGMME
                     FLSVFADKCHHGKEEEFLFPSLEAAGVPNEGGPIGVMLSEHRKGRECIAGMKQGVAAL
                     KSEEKKGIALFADNAAAYTELLRQHIQKED"
     gene            complement(788..>1058)
                     /locus_tag="PK442_15580"
     CDS             complement(788..>1058)
                     /locus_tag="PK442_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOO89127.1"
                     /translation="AEIVDGVVMNENGKNVDAEFRNAVELALEQIKGEAIDLAILQSR
                     SPTCGVNRIYDGTFSGKLIPGQGLFAQALVQRGYTVVDAEDVPKL"
BASE COUNT          206 a          331 c          270 g          251 t
ORIGIN      
        1 gtcttctttc tggatgtgct gcctcagcag ctccgtgtag gccgccgcat tgtcggcgaa
       61 gagcgcgatt cccttctttt cctctgattt cagcgccgcg acgccctgct tcatgccggc
      121 gatgcactcc cgccccttgc ggtgttcgct cagcatgacg ccgatgggcc ccccctcgtt
      181 cggcactccc gccgcttcaa ggctcgggaa gaggaactcc tcctccttcc cgtggtggca
      241 cttgtcggcg aagacggaca agaactccat cattccgtcc aagtgaccta aatcagcggt
      301 ttttccggtc gcgaccttcc gggcaaccgc atcaagaaca tccagcataa cctcgattcc
      361 cctgtgttcg tccatcagtt catccgtcga cttcatagcg ctctcccctt tcgcgggaca
      421 aaaattggtt cttcgtttcg cacagcatat atacagtata gtcagtctct ttgttttttt
      481 caagagaacg ccgcgttccg gcccccggcg aaggattcca aggaacgaag catttgtgca
      541 ataatagaga acatcgccgg gagattcatg acgtatgatt cggtacacgg gaggcgcgac
      601 atgggaatcg aattctatcg cgatgcgagt ttgccgtttt tcgagctgaa actctgcgcc
      661 gaggaggaag tgcactacaa gcggcattcg cacgaggagt gctcccttgg catcctccag
      721 cagggggaga cgctctcggc ggttctttcg cgatcaccgc tcgaacagcg gggcggccaa
      781 gaacggccta cagcttcgga acatcctcgg cgtcgacgac cgtgtacccc ctctgtacca
      841 gcgcctgagc gaagagtccc tgtccgggga tcaacttccc cgaaaatgtt ccgtcgtata
      901 tccggttgac tccgcacgtg gggcttcgtg attgcagaat agccagatcg attgcttcac
      961 cctttatctg ttccaaggca agctcaaccg cgttccgaaa ttcagcatcc acattcttgc
     1021 cgttctcgtt catcaccacg ccgtcgacga tttcggcg
//
LOCUS       DAOSWW010001077         1057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_136871_length_1057_cov_3.397206, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001077 DAOSWW010000000
VERSION     DAOSWW010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136871_length_1057_cov_3.397206"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1057
                     /locus_tag="PK442_15585"
     CDS             <1..>1057
                     /locus_tag="PK442_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOO89128.1"
                     /translation="KGAPASGRYMVVTKSPLNNYIASSNSGGFWGAELARAGWFMIIL
                     EGKARKPSYIWINDDKVEIRDASAFWGLDSHETTDKLLAAVGDDKAKVACIGPAGERL
                     SKLASVMNDKNRAAGRSGVGAVMGSKNLKAIVVKGSSRPHVENQDEMKSVLATAMEKL
                     KTNPVTSQGLPTYGTAVLVNIINQIGGYPYKNWQGAHMPEADSQSGETLARDFLTKRY
                     SCYACPIGCGRVTKVGDREGEGPEYESIFSFGVCCGVEPLEPIIEANYLCNEYGLDTI
                     SAGVTIAAAMELYEKGYIKKEELEGGPELKFGNAEAVTYWTKKMGLVEGFGKKLAMGS
                     YALCEMYGHPEFSMTAKK"
BASE COUNT          244 a          330 c          325 g          158 t
ORIGIN      
        1 aagggagctc ctgcgtccgg acgctacatg gtcgtaacga aaagccctct caacaactat
       61 atcgcgagca gcaactccgg aggattctgg ggcgccgagc tggcgcgcgc cggttggttc
      121 atgatcatcc ttgaaggcaa ggcccggaag ccttcctata tctggatcaa cgacgacaaa
      181 gtggaaatcc gcgacgcctc cgcattctgg gggctcgaca gccatgaaac aaccgacaag
      241 ctcctcgcgg ccgtcggcga cgacaaggcg aaggttgcct gcataggccc cgccggagag
      301 cgcctttcca aactcgcctc cgtgatgaac gacaagaaca gggctgcggg acgaagcggc
      361 gtcggcgccg tcatgggctc gaagaacctg aaggcgatcg tcgtcaaggg aagcagccgc
      421 ccccacgtcg agaaccagga cgagatgaag tccgtcctcg cgactgcgat ggagaagctg
      481 aagaccaacc ccgtcacaag ccaggggctt cccacctacg gcaccgcggt gctcgtgaac
      541 atcatcaacc agatcggcgg ctacccctac aagaactggc agggcgccca catgcccgaa
      601 gccgactcac agtccggcga gacgctcgcc agggatttcc tcaccaagag atactcctgc
      661 tacgcatgcc ccatcggctg cggacgcgtg accaaggtcg gcgaccgtga gggcgagggc
      721 cccgagtacg agagcatctt ctccttcggc gtctgctgcg gcgtggagcc tctggagccg
      781 atcatcgaag cgaactatct ctgcaacgag tacggcctcg acacgatcag cgcgggcgtg
      841 accatcgcgg cggccatgga gctgtacgag aaggggtaca tcaagaagga agagctggag
      901 ggcggccccg aactgaagtt cggcaacgcc gaagccgtta cctactggac gaaaaagatg
      961 ggactcgtcg agggattcgg caagaagctc gccatgggtt cctacgccct ctgcgagatg
     1021 tacgggcacc ccgagttctc catgacggcc aagaagc
//
LOCUS       DAOSWW010001078         1057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_136899_length_1057_cov_2.743513, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001078 DAOSWW010000000
VERSION     DAOSWW010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136899_length_1057_cov_2.743513"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PK442_15590"
     CDS             complement(<1..617)
                     /locus_tag="PK442_15590"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO89129.1"
                     /translation="MLFWILLLAGALGFLGYRVVAPSGAQQRIPPPSAGIMVTLYEVQ
                     PRLWEIWRSFFGTVRSAQVQKVTSQTREVIEEINVEIGDSVKKGDVLISLAGQSQVAL
                     LSARQAAYDDAKSRYDRLQSLFQAGGTSKQELDKANVSVQEEAAKLRDARTTVSRIQI
                     RSALNGIVVRRNAERGEFADPGRELLAIADFDRREVEMLISPTIRG"
     gene            complement(785..>1057)
                     /locus_tag="PK442_15595"
     CDS             complement(785..>1057)
                     /locus_tag="PK442_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89130.1"
                     /translation="CSAGPLYIAFPIAEVMIRKGTSLRNIFVFIGAWSTMRIPMILFE
                     IQNLGAVFGLTRYFVSLVGILLMALLMDRVLNTAEKTEIIARFQEE"
BASE COUNT          245 a          312 c          267 g          233 t
ORIGIN      
        1 cctcgtatgg tgggagagat cagcatctcc acctcccggc ggtcgaagtc ggcgatcgcc
       61 aacagctcgc gccccgggtc cgcgaattcc ccccgctcgg cattcctccg aacgacgatt
      121 ccgttgagag ctgaacggat ttgaatacgg gagacagtcg tcctggcgtc ccgaagcttg
      181 gcggcttctt cctggacgga gacgttcgcc ttgtccagtt cctgctttga tgttccgccc
      241 gcctgaaaga gactctggag acgatcataa cgggatttag catcgtcata ggctgcctga
      301 cgagcgctca aaagcgccac ctggctctgt ccggcaaggg agatgagcac atctcccttc
      361 ttcacgctgt cgcctatctc gacgttgatc tcctcaataa cctcgcgcgt ctgagatgtc
      421 accttctgta cctgcgcgga gcgcaccgtc ccgaaaaagc tgcgccagat ttcccagaga
      481 cgcggctgga cttcgtaaag ggtcaccatg attccagcgg aagggggcgg tattcgctgc
      541 tgcgccccgg aaggcgcaac aaccctatac cctaggaaac cgagggctcc ggcaagaagc
      601 agaatccaga acaacacatt tgctttcctc aataaaacca tggcgtcccc cttgcggttg
      661 caaagcgata acgaatcttt gctctgtcta ccctttccca taatatcttt attgtacatc
      721 cggagctttc tttttcgcta tgcgcaatta cgtaaaactc gttccctata aggaacgaaa
      781 gcgcttactc ctcctgaaaa cgtgctatga tctccgtctt ctctgccgtg ttgagcactc
      841 tgtccataag aagcgccatg agaaggatgc cgacaagcga gacgaaatac cgggtgagtc
      901 cgaagaccgc cccgagattc tggatctcaa agagaatcat cgggatgcgc atcgtagacc
      961 atgcgccgat gaaaacaaag atgtttcgca ggctcgttcc tttacgaatc atcacctcgg
     1021 cgatgggaaa agctatatag agaggccctg cggaaca
//
LOCUS       DAOSWW010001079         1057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_137038_length_1057_cov_1.577844, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001079 DAOSWW010000000
VERSION     DAOSWW010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137038_length_1057_cov_1.577844"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PK442_15600"
     CDS             <1..887
                     /locus_tag="PK442_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89131.1"
                     /translation="NRLDKVLERLETEKDSAPAPSTQRRSFSEGLPVCGLEKKQDTDL
                     LLRLIAEDVSEEHARKLVRDYEAEAPSKDFSEWLAGRIPVAADTNWDALGGGRVMLIG
                     PTGVGKTTTIAKLAAIQSLWEKKNVVFLTADTYRIAAPEQLRTYAKILCVPIDVVYDA
                     NEIPDIVDRYRKADLLLLDTAGRSQKDSRRVEEIRVLYEAFRPDAVHLVLPANMKYRD
                     MLDVIQRMGVVPLSYVLFTKLDETTTYGALLNILLDFDKPVSFFTTGQNVPNDIEVAK
                     GSRLAELLLRGKGDVLRA"
     gene            871..>1057
                     /locus_tag="PK442_15605"
     CDS             871..>1057
                     /locus_tag="PK442_15605"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOO89132.1"
                     /translation="MFFVHEGAAIYDQAGELRRIVQTRRKNIKGLPGLRSIAILSGKG
                     GVGKSNVAVNLAMALAAS"
BASE COUNT          223 a          299 c          322 g          213 t
ORIGIN      
        1 caaatcgtct cgacaaggtg cttgaacgtc ttgagaccga gaaggactct gctcccgctc
       61 catcgacgca acggcgctct ttcagcgagg ggctgcccgt gtgcgggctg gagaaaaaac
      121 aggataccga cctgctcttg cgtctgatcg cggaggatgt gagcgaagaa cacgcccgga
      181 agcttgttcg ggactacgaa gccgaggctc cttcaaagga cttctctgaa tggctggccg
      241 ggagaattcc cgtcgctgcg gacacgaact gggacgctct cggcggaggc cgggtgatgc
      301 tcatcggtcc caccggagtc gggaagacca cgacgatcgc gaagctcgcg gcgattcagt
      361 cgctctggga gaagaagaac gttgtttttc tcactgcgga tacgtaccgg atcgccgccc
      421 cggagcagct gcgaacgtat gcgaagattc tctgcgtgcc gatcgatgtg gtgtacgacg
      481 cgaacgaaat tccggacatc gtcgatcgct acaggaaggc ggatcttctg ctgctggaca
      541 ccgccggccg ctcccagaag gatagccgtc gcgttgagga gattcgggtg ctctacgagg
      601 cattccgtcc ggatgcggtc cacctggtgc ttcccgcgaa catgaagtac cgcgacatgc
      661 tcgacgtcat ccaacggatg ggcgttgttc cgctttcgta cgttcttttc acgaagcttg
      721 acgagacaac gacctacggc gccctgctga atatcctgct tgacttcgac aaaccggtct
      781 cttttttcac cacagggcag aacgtcccga acgatattga agtggccaag ggctcacgcc
      841 ttgccgaact cctccttcgc gggaagggag atgttcttcg tgcatgaggg tgcggcgatc
      901 tatgatcagg cgggagaact tcgcagaatt gtacaaacac gccgtaagaa catcaagggg
      961 ctgccgggcc tccgttccat cgccattctc agtggaaaag gcggggtagg aaagagcaac
     1021 gtcgcggtga atctcgccat ggcgctcgct gcttcag
//
LOCUS       DAOSWW010001080         1055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_137276_length_1055_cov_4.766000, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001080 DAOSWW010000000
VERSION     DAOSWW010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137276_length_1055_cov_4.766000"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1055)
                     /locus_tag="PK442_15610"
     CDS             complement(<1..>1055)
                     /locus_tag="PK442_15610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOO89133.1"
                     /translation="GTLDKIFRQYRSKRIVIASFASNLHRVQQVVDCAARFNRKVAFV
                     GRSMVKNVELARTLGYLHVDDKIIVDMEDISSVPSSSLVVMTTGSQGEPFSGLVLMSR
                     GEHRVISLSDKDVVALFATPIPGNEKLVSGTVNRLFACGCDVVYEKEQGIHVSGHAAK
                     DELRVMLNMVKPKFFVPVHGEYRHLVRHAQLARETGVATRNAFVLQNGDVFQLDGEKA
                     AIKERVQAGGIMVDGLALGELQGSIMKERRELSEEGLVLVSIVLSGAGELLASPSFES
                     RGFLHLDDAAQLKEDLIASVEKTVLSLGAKSVSDIDLLETKIKGRIRDTLRRFGRSKP
                     MITPLITVLGAPEKNGK"
BASE COUNT          217 a          335 c          280 g          223 t
ORIGIN      
        1 cttcccgttc ttttccggag cgccaagaac cgttataaga ggtgttatca tcggtttact
       61 tcgtccgaag cggcgaagcg tgtcgcgtat ccgccccttt atttttgtct ccaggagatc
      121 gatatcggag acagacttcg cgccgagaga gagcaccgtc ttctccaccg aggcaatcag
      181 gtcctccttc aactgcgccg cgtcgtcaag gtgcaggaac ccccggctct cgaaagaagg
      241 agacgcaagc agctcgccgg cgccggagag tacgatggaa acgaggacaa gcccctcttc
      301 cgaaagttcc cgccgctcct tcatgatact gccttgaagc tctccgagcg ccaacccgtc
      361 aaccatgata cccccggctt gcactcgctc tttgatcgcc gccttctccc cgtcgagctg
      421 gaacacgtcg ccgttctgga ggacaaaggc gttccgcgtc gcaacgccgg tctcccttgc
      481 aagctgggcg tgccggacaa gatggcggta ctctccgtga accggaacga aaaatttcgg
      541 cttgaccata ttgagcatca cgcgcagttc gtcctttgct gcatgaccgg agacgtgaat
      601 tccctgctcc ttctcgtaca caacgtcgca cccgcaggcg aagaggcggt tgaccgtccc
      661 gctcaccagc ttctcgtttc cagggatagg agttgcgaag agcgcgacca cgtccttgtc
      721 gctcaagctg atcacacggt gctcgccccg gctcatcagc accagtccgg agaagggctc
      781 tccctggctg cccgtagtca tcacaacgag cgaacttgaa gggaccgagg agatatcctc
      841 catatcgacg ataatcttgt catccacgtg cagatacccg agggtccgtg caagctcgac
      901 gttcttcacc atgcttcttc cgacgaaagc gacttttcgg ttaaaacggg ctgcgcagtc
      961 caccacttgc tgcacacggt gcagattgct cgcgaacgag gcgatgacga tacgcttcga
     1021 ccggtactgt ctgaagatct tgtcgagcgt tccgg
//
LOCUS       DAOSWW010001081         1055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_137279_length_1055_cov_3.853000, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001081 DAOSWW010000000
VERSION     DAOSWW010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137279_length_1055_cov_3.853000"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1055
                     /locus_tag="PK442_15615"
     CDS             <1..>1055
                     /locus_tag="PK442_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HOO89134.1"
                     /translation="LHEKPFAGLNGSGKHVNFSIMDSEGRNLLKPSTNHRKNIQFLTF
                     LSALLLGFGKYYGLIRASIASPGNMHRLGGNEAPPAIMSAYLGSAITGILEAVEKGLP
                     NDLPAQALIDLGMNKLPSIMADNSDRNRTAPLAFTGNKFEFRAPGAPQSIAGPLTMLL
                     AAWAWGIDRVADMIEAKGSDVDVLEAALDAIKYAAAESRAIRFEGNAYSAEWQQEAKK
                     RGLKIANSTQEALALYLVPEHRELLASLGIMSEREVVAYYEVRLEQYIKTVSIEMGIM
                     RAMLWEGILPAISRQILLEQGALSSLPEEMIKESVLWKKSVANLVSIKNGLIAASEKL
                     DAMLAKIHELEIDEQSR"
BASE COUNT          264 a          295 c          291 g          205 t
ORIGIN      
        1 cttcacgaaa agccgttcgc agggctcaac ggcagcggga aacatgtcaa tttctctatt
       61 atggacagtg aagggcgcaa ccttctgaaa ccgtcgacga accataggaa gaacatccag
      121 ttcctcactt ttttaagcgc tctccttctt gggttcggga agtactacgg ccttatccgg
      181 gcttcgatcg cctctccggg aaatatgcac cgtctcggcg gaaacgaagc cccgcccgcc
      241 ataatgagcg cataccttgg ttcagctatt accggaattt tggaagccgt tgagaaagga
      301 ctgccgaacg atctccccgc gcaggcgctg atcgatctgg ggatgaacaa actgccctcc
      361 atcatggcgg ataattccga ccgcaacagg actgcgccgc tcgccttcac cgggaacaag
      421 tttgaattcc gggcgccggg cgccccgcaa tccatcgccg gtccgctgac gatgcttctt
      481 gcagcctggg catggggaat cgatagggtc gccgacatga tcgaagcaaa gggttccgac
      541 gtcgacgtcc ttgaagcggc gctcgacgcg atcaagtatg cagcggctga aagccgtgca
      601 atccgcttcg aaggcaacgc ctacagcgcg gagtggcagc aggaggcgaa gaaaagggga
      661 cttaagatag cgaacagcac ccaggaagcc ctggcgctct atcttgtacc cgaacaccgg
      721 gaactgctcg caagcctcgg catcatgagc gaacgagagg ttgtcgcgta ctacgaggtt
      781 cgccttgaac agtacataaa aacagtctcc atcgaaatgg gaatcatgcg ggccatgctc
      841 tgggagggga tactcccggc gatatcccgt cagatactcc tggaacaggg agcgctctct
      901 tcgctacccg aagaaatgat caaggaatcg gttttgtgga aaaagagcgt tgccaacctg
      961 gtttcaataa agaacgggtt gatcgccgct tccgagaaac tcgacgcaat gcttgccaag
     1021 atacacgaac tcgaaatcga tgaacagtct cgcat
//
LOCUS       DAOSWW010001082         1055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_137317_length_1055_cov_2.761000, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001082 DAOSWW010000000
VERSION     DAOSWW010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137317_length_1055_cov_2.761000"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK442_15620"
     CDS             <1..399
                     /locus_tag="PK442_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89135.1"
                     /translation="MDFCVTDSSFRGNSIQFFTYLFTESLMYPNRYHLHTTVSPKNIF
                     SLTNVLKCGFSAIKFKQKYGGHHRFVLYKNLRKPGAVKTRGHKEILLRNYDYHPKVFA
                     DGFVGYKIKHKSNGMAMLYGKPLEGAPSKA"
     gene            399..>1055
                     /locus_tag="PK442_15625"
     CDS             399..>1055
                     /locus_tag="PK442_15625"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOO89136.1"
                     /translation="MNSVPAQGMETLRLLERAGFESVLVGGAVRDLLLGRTPGDIDIA
                     TEASFSDLAALFPGGKSVGPEGKTVFLVPCGEGRCEITSFCGGSLETDLARRDFTVNA
                     LALRSTGELVGTRRSKLDIELRLLRFNGSAADRLREDPLRALRLPRFAATLPGFSVAG
                     GAYSGVRAAAPSFARCAPERIGKEVRFGLEGDPGLFLRGLAASRTLHILRSLFGGGPK
                     G"
BASE COUNT          197 a          307 c          334 g          217 t
ORIGIN      
        1 atggattttt gcgttacgga ctcatccttc aggggcaaca gcattcagtt ctttacgtat
       61 cttttcaccg agagccttat gtacccgaac agataccatc tgcacaccac ggtgtcgccg
      121 aagaatatct tcagcctgac aaacgtgctg aagtgcggat tttccgcgat caagttcaag
      181 cagaagtacg gcgggcatca ccgtttcgtg ctctataaga acctgaggaa acccggcgcg
      241 gtgaagacgc gggggcacaa ggagatactc ctcagaaact acgactacca tccgaaggtc
      301 tttgccgatg gcttcgtagg gtataagatc aaacacaagt cgaacggaat ggcgatgctc
      361 tacggcaagc ctctggaggg agcgccgtcg aaggcgtgat gaacagcgtg ccggcgcagg
      421 gaatggagac gctccgtctg cttgagcgcg ccggtttcga aagcgttctc gtggggggcg
      481 ccgtgcggga tcttcttctt ggacggactc ccggggatat cgacatcgcg acggaggcct
      541 ccttttccga tctcgccgcg cttttccccg gcgggaagag cgtcggacca gagggaaaga
      601 cggtctttct tgttccctgc ggagaggggc gctgtgaaat cacatccttc tgcgggggct
      661 cacttgaaac ggacctggcg cgccgtgact tcacggtgaa cgcgctcgcc ctccgctcca
      721 caggcgagct tgtcggaacc cgccgcagca aactggatat cgagcttcgg ctgctgcgct
      781 tcaacgggag cgcagccgac cggctcaggg aagatccgct cagggcgctt cgtttgccgc
      841 gtttcgcggc gacgcttccc ggcttttccg tggccggggg cgcctattcc ggcgtgaggg
      901 ccgcggcgcc gtcgtttgcc cgttgcgccc ccgaacggat aggaaaagaa gtgcgtttcg
      961 ggctcgaagg cgatccgggg cttttcctgc gcggtctggc ggcaagccgc acgcttcata
     1021 ttctccgttc cctgttcggc ggcgggccga agggg
//
LOCUS       DAOSWW010001083         1046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_139300_length_1046_cov_3.209889, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001083 DAOSWW010000000
VERSION     DAOSWW010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139300_length_1046_cov_3.209889"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1046
                     /locus_tag="PK442_15630"
     CDS             <1..>1046
                     /locus_tag="PK442_15630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOO89137.1"
                     /translation="AALQPVERSIVPGDVLVEKGQIITDQAARILEMQGYSRVSFPWK
                     QILFALVVIPFWPLWLRIQTRNHGGGEEKSELPWVYLAFIVGVSWLAEYVSALFHAQG
                     MGSLFLAGCAYLTLPAHLALQLVLGGAILGGLVVTGFSTLHVLLVALMGILSAFGGYF
                     LLIGVHSRGHLWKQLFLLGVLQTLTGLSLRWAFNISFSAEILIPLILGSALWSSLVIA
                     ALPLLEDTFDVLSPLRLMELSHPSNPLLKRLQMEAAGTYHHSLMLGTLAEAVADKLGM
                     NANLLKAGAYFHDIGKLRRPDFFVENQMGGENVHDELKPSLSALVIIAHVREGLELSH
                     EYHLPRVIRSFIAE"
BASE COUNT          182 a          314 c          305 g          245 t
ORIGIN      
        1 gggcggcgct gcagcctgtg gaacgttcca tcgttccggg cgacgtgctc gttgaaaagg
       61 ggcagatcat caccgatcag gccgccagaa tcctcgaaat gcagggctac tccagggtgt
      121 cttttccgtg gaaacagata ctcttcgcgc tcgtggtcat ccccttctgg cctctttggc
      181 tccgcatcca gacgcggaat cacggcggcg gcgaagaaaa aagcgaactt ccgtgggtct
      241 acctcgcctt tatcgtcggc gtcagctggc ttgcggagta cgtctcggcc ctttttcacg
      301 cccaggggat ggggagcctc ttccttgcag gatgcgcgta tttgacgctt cctgcgcacc
      361 ttgcgcttca gcttgttctt gggggagcaa tcctcggcgg tcttgtggtc accgggtttt
      421 ctacgctcca tgtgctgctt gtggcgctca tgggaatact cagcgcattc ggagggtatt
      481 ttctccttat aggcgtccac tccagagggc acctctggaa gcagctcttt cttctcggcg
      541 tccttcagac gcttacggga ctctcgctca gatgggcgtt caacatctcc ttttccgcgg
      601 agatattgat accgctcata cttgggagcg ccctctggag cagcctcgtc atcgcggcgc
      661 tccctcttct tgaggatacg ttcgacgtgc tttcgccgct tcgcctgatg gagctgagcc
      721 atccgtcgaa tccgctgctc aagaggttgc agatggaggc cgcgggaacg taccatcact
      781 cccttatgct cgggacgctg gcggaggcgg tggccgacaa gctcgggatg aacgcgaacc
      841 tgctgaaagc gggcgcgtat ttccacgaca tcggaaagct gcggcgcccg gacttcttcg
      901 tcgaaaacca aatgggcggc gagaacgtcc atgacgaact caaaccatcg ctctccgcac
      961 tggtcatcat cgcgcacgtc cgtgaagggc tggagctttc gcatgaatac cacctgccga
     1021 gggttatccg ttcgttcatc gcggag
//
LOCUS       DAOSWW010001084         1045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_139519_length_1045_cov_3.850505, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001084 DAOSWW010000000
VERSION     DAOSWW010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139519_length_1045_cov_3.850505"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /gene="pyrF"
                     /locus_tag="PK442_15635"
     CDS             <1..657
                     /gene="pyrF"
                     /locus_tag="PK442_15635"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOO89138.1"
                     /translation="RELLASLKGELKYVKIGHQLYARGGFPFLREIGAMGFSIFLDLK
                     LHDIPNTVFQATEALASENLWALTLHCGGGRKMLEEARRGKEKSGSRTNLLGVTVLTS
                     FDDASWAEVAPGCSLSDALEKRAALCRETGIDGIVCSPLDLPVVRAAGAGLFTVVPGV
                     RPASADDDQARVATPQKTIANGADYIVVGRPILNAPDRIAAVRSIAASIEEGLKCRSL
                     "
     gene            642..>1045
                     /locus_tag="PK442_15640"
     CDS             642..>1045
                     /locus_tag="PK442_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOO89139.1"
                     /translation="MQESVMLAKLQEMMKESGAHLEGHFLLTSGNHSSDYMQCALLLR
                     FPRYAAFAGEALAEKVRRYSPDLIASPALGGLIIGHEVARALDVPFIFCERREGEMVL
                     SRFPFPEGKRVVVIEDVVTTGVSSGEVGSVLER"
BASE COUNT          178 a          334 c          348 g          185 t
ORIGIN      
        1 cgcgagcttc tcgcctcgct caagggagag ctgaagtatg taaagatagg ccatcagctc
       61 tacgcccgcg gaggatttcc cttccttcgc gagatcggcg cgatggggtt ctcgatcttc
      121 ctcgacctca agctgcacga tattcccaac accgtcttcc aggcgacgga ggcgctcgct
      181 tctgagaacc tgtgggcgct cacgctgcac tgcggcggcg gccggaagat gctggaagag
      241 gcccgtcgcg gcaaagagaa gtcggggtcg cggacgaacc tgctcggcgt gaccgtcctc
      301 acgagcttcg acgacgcctc gtgggcggag gtggcccccg ggtgttcgct ttccgacgcg
      361 ctcgaaaaac gcgccgcgct ctgccgggag acaggtatcg acggcatcgt ctgttctccg
      421 ctcgaccttc ccgtggtccg cgccgccgga gccgggctct tcacggtcgt cccaggagta
      481 cgtccggcga gcgccgacga cgaccaggcg cgcgtcgcca cgccgcagaa aacgatcgcg
      541 aacggcgcgg actacatcgt cgtggggcgt ccgatactga acgctccgga cagaatcgcg
      601 gcggtgagaa gcatcgccgc gtcgatagag gaggggctga aatgcaggag tctgtaatgc
      661 tggcgaagtt gcaggagatg atgaaggaga gcggcgcgca ccttgaaggc cactttctgc
      721 tgacttcggg gaaccacagc agcgactaca tgcagtgcgc cctgctgctt cgctttccgc
      781 gctacgccgc cttcgccgga gaggcgcttg cggagaaggt gcgccgctac tcgcccgacc
      841 ttatcgcctc gcccgctctc ggcggcctca tcatcgggca cgaggttgcg cgcgcgctcg
      901 acgttccctt catcttctgc gagcgaagag agggcgagat ggtcctcagc cgcttcccgt
      961 tccccgaggg gaagcgcgtc gtggtgatcg aggatgtggt taccaccggc gtctcctccg
     1021 gcgaggtggg aagcgttctc gaacg
//
LOCUS       DAOSWW010001085         1041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_140387_length_1041_cov_2.922921, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001085 DAOSWW010000000
VERSION     DAOSWW010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140387_length_1041_cov_2.922921"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK442_15645"
     CDS             <1..227
                     /locus_tag="PK442_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO89140.1"
                     /translation="LDMEVERLVQKAMNKAMEGRTSFVIAHRLSTVRNADRIVVLEEG
                     RIAEQGTHEELLARNGLYHRLCALGETAAP"
     gene            224..>1041
                     /gene="lpxK"
                     /locus_tag="PK442_15650"
     CDS             224..>1041
                     /gene="lpxK"
                     /locus_tag="PK442_15650"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOO89141.1"
                     /translation="MNGAGGVRRRFSPANVIQSIRSSVGVALGSYLSFARGETRFSPW
                     ILVSPFGLIARMVVAGRNFAFDHGLIRSEEPPLPVVSVGNITLGGTNKTPFVEMLCRM
                     FQEAGVTPGIISRGYGGRTVDPVVITAEEISAGAASEKTLSRYRDLVGDEPLLLASRL
                     PGVSVAVSKDRLRDVDVLSERNVQLIVADDAFQHRRMGRDADIVLVDACCPFGNGWIA
                     PAGILRESPSVLARASAVVVTKSDQVEPERLERLVGELSRFVPKERLFFSRISLL"
BASE COUNT          176 a          316 c          334 g          215 t
ORIGIN      
        1 ctctcgacat ggaggtcgag cgtctggtcc agaaggcgat gaacaaggcc atggagggac
       61 gcacctcttt tgtcatcgcg catcgcctct ccacggtccg gaacgcggac aggatcgtcg
      121 tgcttgaaga agggcgcatt gccgagcagg ggacgcatga agagctgctc gcgcgcaacg
      181 gcctctatca ccgcctgtgc gccctcgggg agacagctgc tccatgaacg gcgcgggagg
      241 cgtgcgaagg cgtttctctc cggcgaatgt gatccaaagc atccgctctt cggtcggcgt
      301 cgccctcgga agctacctct ccttcgcccg cggagaaacg cgcttttccc cgtggattct
      361 tgtctctccg ttcggcctga ttgccaggat ggttgtcgcc ggtcgaaatt tcgctttcga
      421 tcacggcctg atccgctcgg aggagcctcc gcttccggtg gtgagcgtcg ggaacatcac
      481 gctgggaggg acgaacaaaa cgccgttcgt cgagatgctc tgccgtatgt tccaggaggc
      541 gggcgtgacg cccggtatca tcagcagagg gtacgggggg cgaaccgttg atcctgtagt
      601 aataactgcg gaggagatat ccgccggggc ggcttccgaa aagacgctct cccgatacag
      661 agatcttgtc ggcgacgaac cgcttcttct ggcttcgcgc ctcccggggg tttccgttgc
      721 ggtctccaaa gatcgtctgc gcgacgtcga cgtcctttcc gagcggaacg tacagcttat
      781 cgtggccgac gacgcctttc agcaccgaag gatggggcgc gacgcggaca tcgtgcttgt
      841 cgacgcgtgc tgcccgttcg gcaacggatg gattgcgccg gccggtattc tcagagaatc
      901 gccgtcggtg cttgcgaggg cgtccgccgt cgtcgtcacc aagtcggacc aggtagaacc
      961 cgagcgcctg gagcgtcttg tcggggaact ttctcgcttc gtcccgaagg aacggctgtt
     1021 cttctcacgc atctcgctgc t
//
LOCUS       DAOSWW010001086         1039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_140864_length_1039_cov_2.825203, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001086 DAOSWW010000000
VERSION     DAOSWW010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140864_length_1039_cov_2.825203"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..>1039
                     /locus_tag="PK442_15655"
     CDS             96..>1039
                     /locus_tag="PK442_15655"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HOO89142.1"
                     /translation="MLSQKIDAMKDELIAALQESVRIPSVGGDPEEGAPFGAGPRKSL
                     DWTLAFAEKMGFRTKNVENVAGWAEMGEGDEMVAVLGHLDVVPEGKGWTVDPWGGELK
                     DGMIYGRGVLDNKGPVMVGLFAAKAIFDSGIALKRRIRVIFGTNEERGSKCMKRYVEV
                     GEELPAAGFTPDAEYPIIYAEKGILTYTLSLPFAPSGEMKLLSLEGGVAANVVMAEVK
                     AEITDPRPECRQRIMDAAAAWKGPEGSSLSAAEDAAQHIVTLEMKGHPAHGSTPEKGI
                     NALACLADFMNGLRLKGEQGDFFARFSKLIGFETDGASL"
BASE COUNT          223 a          281 c          339 g          196 t
ORIGIN      
        1 tcccgcactt cgcagatcgt atgtgcgagg ttttccggga gcgctctctt tttatgaatg
       61 ttgtataata gatatcattt ttaggaggaa ttgtcgtgct ctctcagaaa atcgatgcaa
      121 tgaaagacga actgatcgcc gcgcttcagg aaagcgttcg tatcccaagc gtaggagggg
      181 atcccgagga aggcgctcca ttcggcgccg ggccccgtaa gtcgctcgac tggacgcttg
      241 cgttcgccga gaagatgggg ttcaggacga aaaacgtgga gaacgtcgcc ggatgggcgg
      301 agatgggcga gggcgacgag atggtcgcag tgctcgggca tctcgacgtc gttcccgagg
      361 ggaagggatg gacagtcgat ccctggggcg gcgaactgaa ggacggcatg atctacggca
      421 ggggtgttct tgacaacaag ggccccgtca tggtgggtct tttcgccgcg aaggcgatct
      481 tcgactcggg catcgcgctg aagcgcagaa tccgcgtgat cttcggaacg aacgaggagc
      541 ggggaagcaa gtgcatgaaa cgctacgtcg aggtcggaga ggaactgcct gcggccggat
      601 tcaccccgga tgcagagtac cccatcatct atgccgagaa gggaattctt acgtacacgc
      661 tctctcttcc gttcgcaccg tcaggcgaga tgaagctgct ctctctcgaa ggcggcgtgg
      721 ctgcaaacgt cgtcatggcg gaagtcaagg cggagataac cgaccctcgt cccgagtgca
      781 gacaacgcat catggacgcg gcggccgcct ggaaaggccc cgaggggagt tcgctcagcg
      841 cggcggagga tgctgcgcaa catatcgtga cccttgagat gaagggccat cccgcccacg
      901 ggagcacccc tgagaagggc atcaacgcgc tcgcctgcct cgccgacttc atgaacggtc
      961 tcagactgaa aggggagcag ggggatttct tcgcccgctt ctcgaagctt ataggcttcg
     1021 aaaccgacgg cgcctcgct
//
LOCUS       DAOSWW010001087         1038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_141102_length_1038_cov_2.934893, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001087 DAOSWW010000000
VERSION     DAOSWW010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141102_length_1038_cov_2.934893"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1038)
                     /locus_tag="PK442_15660"
     CDS             complement(<1..>1038)
                     /locus_tag="PK442_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89143.1"
                     /translation="QMAADWLKQFPVQSASVVVGLTDKGFSLQGAARVDESKKELLQK
                     LAEGKGEEGDIDLLINSPVPGALILAPVEGNVYAVVADGVAGVLLSVEGDMVLFGFGP
                     EDIEAAKAALADPAKRMNMQRRLPQKNFYFFHDNGMAAAELIAQSKGTLEEPVENLFA
                     EIGFGLTEKGFDLSVFTNFAKTFGLDKLETEIRPLSKDDLFLVGGGNAWLTMAGRVVM
                     EKRHFDMIREAAEGGDQDAAQAVEVLEQLKVMGLTENALISILKSVGIVLGGESSFSG
                     MPIPGGYLYTSGEKDWVELLLPLLEAIVKESGGEFEPLTLPGWQALYVMRDPVDVVLG
                     IRDGIALAGFLS"
BASE COUNT          219 a          320 c          278 g          221 t
ORIGIN      
        1 gctgaggaat ccggcgagcg cgataccgtc gcgaatacca aggacgacat ccacaggatc
       61 cctcattaca taaagcgcct gccagcccgg gagcgtcagg ggctcgaatt ctccgccgct
      121 ctctttcact atggcttcga ggagcggcag taggagctca acccaatctt tttcaccgga
      181 ggtgtacagg tacccgccgg gaataggcat accggagaag gaagattctc cgccgaggac
      241 tatgccgacg gacttgagga tggagatgag cgcgttctct gtaagtccca tcactttcag
      301 ctgttcgagc acttccacgg cctgcgcagc atcctggtct ccgccttctg cggcttcacg
      361 gatcatgtcg aagtgcctct tctccataac aacccgcccg gccatagtca gccatgcgtt
      421 cccgccgccg acaaggaaca gatcgtcttt tgagagcggg cgaatttccg tctccagctt
      481 gtccaatccg aacgtcttgg cgaagttggt gaacacggag agatcaaaac ccttttctgt
      541 aaggccgaag ccgatctccg caaagaggtt ttctaccggt tcttccagcg tccccttgga
      601 ctgcgctatg agttcggcag ccgccattcc gttgtcatgg aagaaataga agtttttctg
      661 ggggaggcgc cgctgcatgt tcatacgttt cgccgggtcg gcgagagccg cctttgccgc
      721 ctcgatatct tcgggaccga aaccgaacag caccatatct ccctcgacgg agaggagaac
      781 gccggcaact ccgtcggcca caaccgcata gacattcccc tcgaccggag cgagaatcaa
      841 cgcgccggga accgggctgt tgatcaggag atcgatatcc ccttcctctc ccttgccttc
      901 ggcaagcttt tgaagcagct cttttttgct ttcgtcaacc ctggcggcgc cctggaggga
      961 gaatcctttg tcggtaaggc caacgacaac agacgccgac tgcacaggga actgtttcag
     1021 ccaatccgcc gccatctg
//
LOCUS       DAOSWW010001088         1038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_141103_length_1038_cov_2.928789, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001088 DAOSWW010000000
VERSION     DAOSWW010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141103_length_1038_cov_2.928789"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PK442_15665"
     CDS             <1..823
                     /locus_tag="PK442_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOO89144.1"
                     /translation="WKLTSGGSVQGVAGWSRTRETVQRALDTGIITHACDSAEECAAL
                     GDILILAVPIRSMEETSLSIAGAVRQDALAVFDLASTKTEVGRSLSSIWGPRYAGFHP
                     MAGKEKGGIGNADPELFLGSICAIVPFPGTSPSVLTLAEELASALGGRSLRTDPAEHD
                     AVTACISHFPVLVAASLSLLAGKEREIHPLLPLLAAGGFRDTTRVAGGPAWLGADMAS
                     TNGVQVRRLAEEFKSVLDELLSASPEELEILLAKASECRESILAAKEAWRKEERQ"
BASE COUNT          192 a          298 c          326 g          222 t
ORIGIN      
        1 atggaagttg acgtccggcg ggagcgtgca aggcgtcgcc ggctggagcc gcactcggga
       61 aaccgtgcag cgtgctctcg acacaggcat cataacgcat gcctgcgatt ctgccgagga
      121 gtgcgcggca cttggtgata ttctgatact cgcagtgccc atccgttcga tggaggagac
      181 gagcctaagt atcgccggcg cggtgcgtca ggacgcgctc gctgtttttg atctcgccag
      241 cacgaagacc gaggtggggc gttcgttatc atctatctgg ggacctcgat acgcggggtt
      301 tcaccccatg gcggggaagg agaagggggg gatcggtaac gcagaccccg aactcttcct
      361 tggttccatc tgtgccattg tccccttccc cgggacctcc ccttccgttc ttaccctggc
      421 ggaggaactc gcttcggcgc tcggggggcg ttctctccga acagatccgg ccgaacacga
      481 cgcggtgact gcctgtatca gccactttcc ggtgctcgtg gccgcatcgc tctccctgct
      541 tgccggaaag gaacgggaga tccacccgct cctgccgctt cttgcagcgg gaggttttcg
      601 tgataccacg cgcgttgccg gcggccccgc atggctcggc gcggacatgg cgagcaccaa
      661 cggcgtacag gtgcgccgcc tggcggagga gttcaaaagc gtattggacg agctgctctc
      721 cgcttctccg gaagagctgg agattttgct ggcgaaggct tcggagtgcc gcgagtcgat
      781 ccttgctgcg aaggaagcat ggagaaaaga ggagagacag taaatctcgt caaagcagca
      841 gcgcctcttc tttctttcgt caatactatg ggcgttgaat agagtgccgg gagaaatagc
      901 ttcgtttgat accggatttc gcgggcatga ggaaggagat ctcctctcca aaagaattca
      961 caggcgcctt ccgctgattc gcgcgaagct tgttcctctc gtatcgtgtt tctcgccgtc
     1021 ggacgaacgt atcaggta
//
LOCUS       DAOSWW010001089         1038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_141230_length_1038_cov_1.743642, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001089 DAOSWW010000000
VERSION     DAOSWW010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141230_length_1038_cov_1.743642"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PK442_15670"
     CDS             <1..400
                     /locus_tag="PK442_15670"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO89145.1"
                     /translation="RRGRDRAYVAGARRLIGGYELMFKVNESEFEYRFGDSGPKYLMR
                     GPRMNFGVVVLQAGQDFRDHLHNVMEENFFILEGELEFRVADQVLTAKAGDLVHVEPG
                     EAHYLRNASDAPAKAVFCLAPFQEKDKVDL"
     gene            583..>1038
                     /locus_tag="PK442_15675"
     CDS             583..>1038
                     /locus_tag="PK442_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO89146.1"
                     /translation="MVEQNGPAPLVEVEGMVKSFGENAVLRDVSLALRRSDVMAVVGG
                     NGAGKSTLMKILAGIYTPDKGSIRIEGSDVSLHTPSAAHAMGVYLVPQEPLLFPNMTV
                     EENLSLGLDEPRTDVRKRAVALIKRLNWPLSMSRQANSLSIAEQQLVEIL"
BASE COUNT          190 a          311 c          358 g          179 t
ORIGIN      
        1 ccgacgaggg cgtgaccgcg catatgtggc gggcgcccgg cgtctgattg gagggtatga
       61 gctaatgttc aaggtcaacg agagcgaatt cgagtaccgg ttcggcgaca gcggccccaa
      121 gtacctgatg agaggccccc gcatgaactt cggcgtggtg gtgctccagg cggggcagga
      181 cttccgggac catctgcaca acgtcatgga ggagaacttc ttcatcctcg aaggggagct
      241 ggagttccgc gttgccgacc aggtgctgac ggccaaagcg ggcgacctgg tgcacgtcga
      301 gccgggcgag gcccactatc tgcggaacgc ctcggacgcg cccgcgaagg cggtcttctg
      361 cctcgcgccg ttccaggaga aggacaaagt cgatctctga ttcgcgggag taggcggccg
      421 tctcctttcg ggagctcgcc gcgcggcgtg ggcattgact tgaaggacgc tcggggacaa
      481 cgcccttttt cgcaagccgg cggttcggga gaggacggtt tctcttccgg cggcgcattc
      541 cggcgatgcg gcgaaacgca atagaatctg cggggtgatc gtatggtgga acagaacggg
      601 cccgcccccc tcgtcgaagt ggaggggatg gtcaagtcct tcggagagaa cgccgtgctg
      661 cgcgacgtct ctctggcgct ccggcgttcg gacgtgatgg cggtagtcgg gggaaacggc
      721 gccggaaaga gcacgctgat gaagatcctc gccggtattt acacgccgga caagggaagc
      781 atccgcatcg aggggagcga cgtctcgctg cacacccctt cggcggcgca tgcgatgggg
      841 gtgtacctcg tcccccagga gccgctgctc ttccccaata tgacggtgga ggagaatctg
      901 agcctcgggc tcgacgaacc caggaccgac gtccgcaagc gcgccgttgc gctgatcaag
      961 cgtctcaact ggccgctctc gatgtcgcga caggccaact ccctctccat cgccgagcag
     1021 cagctcgtcg agatactg
//
LOCUS       DAOSWW010001090         1034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_141996_length_1034_cov_4.657814, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001090 DAOSWW010000000
VERSION     DAOSWW010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141996_length_1034_cov_4.657814"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PK442_15680"
     CDS             complement(<1..790)
                     /locus_tag="PK442_15680"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOO89147.1"
                     /translation="MGNRDNAFQRLLADEHLFVRFIRRYLRQHMPDAIDVETLTPEDV
                     YREDITFIPPELSKRQSDVLYRIRRGSADVYIYVLIEHQSSVNYLMPFRLLSYMVQFW
                     RRCVEKAGRAAERKDFMLPPVLPVVFYEGEKRWTVSPHFIDKVENARDFGNHVPSFEY
                     LLISLREKTPEEILSIKDALGAMFYLANPAKAENFDEAAERVRLFLRGLSKEERDLTT
                     NHLRGYLKILAEKEGLMIDEALDLCLEREEADIMLAYMQKEFRKA"
BASE COUNT          252 a          330 c          226 g          226 t
ORIGIN      
        1 gcgccttcct gaactccttc tgcatgtagg ccagcatgat atccgcctcc tcccgttcca
       61 aacaaagatc cagagcctcg tcgatcatca gcccttcctt ttcggcaagg atcttcaaat
      121 accccctcaa atggttcgtc gtcaaatctc tctcttcctt cgaaagcccc cgaagaaaaa
      181 gccgcacgcg ctccgcagcc tcgtcgaaat tctccgcctt cgcaggattc gccagataaa
      241 acatcgcccc caaagcatct ttgatcgaca gtatctcctc gggcgtcttc tcgcggagcg
      301 agatcagaag atactcgaaa ctcgggacgt gattcccgaa gtcgcgcgcg ttctccacct
      361 tgtcgatgaa gtgcggcgac accgtccacc gtttttctcc ctcatagaac accaccggca
      421 ataccggagg cagcataaaa tccttccgct cggcagccct tcccgccttt tccacacaac
      481 gtctccagaa ttgcaccata tacgaaagaa ggcgaaaagg catcaggtaa ttcacgctcg
      541 actgatgctc gatcagcaca taaatataca catcggcgct ccctctccgg atgcggtaca
      601 aaacatccga ctgacgcttg gagagttccg gaggaataaa ggtgatatcc tcccggtaca
      661 catcttcagg cgtcaaagtc tccacgtcga tcgcatccgg catgtgctgt ctcagatagc
      721 ggcgaatgaa cctcacgaag agatgttcgt ccgctaaaag ccgctggaac gcattatccc
      781 tgttccccat cgggctcccc ttcacttgca gtatgtttga ttattatagc acctgctgaa
      841 tgccccctta caacgacggc gcttccgtat tcgcatcgcg ctgtgctatc attatacttg
      901 caaagggggc ggcgcagtga gcgacgacat aaagagcgac aaccccttcg atctcgcgca
      961 ctacgccgga atgcaggcat ggaaacaacg cggttccgat acacgcaaac tggactacat
     1021 gaaactcctg ctcg
//
LOCUS       DAOSWW010001091         1034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_142067_length_1034_cov_2.444331, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001091 DAOSWW010000000
VERSION     DAOSWW010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142067_length_1034_cov_2.444331"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1034)
                     /locus_tag="PK442_15685"
     CDS             complement(<1..>1034)
                     /locus_tag="PK442_15685"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOO89148.1"
                     /translation="EQQGILFLGSSESVLDTQSEFLTVDSRLKIYRKQGTITSRPLYS
                     LLPEKKKATAGSERGSSKMPLSSLYPLLLERCMPTGFLVNSKGDLLHTFGGAGKYLQI
                     IGPVRTDIGSILHGNLRVAVMTAIERSRKNRTPVRFSDVSHPLPGAPLNTVEVSVEPL
                     EERTDSRSEEGNLFFFIRLEEMKPSLAAKAESFSVDEAAKVRIEELERSLSEARENLQ
                     TIVEELETSNEELQATNEELLASNEELQSSNEELQSVNEEMHSLNAEYQEKNEQLIDL
                     NNDLQNLMSCTDIGTIFLDGELRIRRFTPAINRVFFLRDQDIGRPIEEMRSLVESGHD
                     VVASIRRVLR"
BASE COUNT          204 a          312 c          287 g          231 t
ORIGIN      
        1 ccctgagaac gcgcctgatc gacgcgacaa cgtcgtgtcc gctctccaca aggctgcgca
       61 tctcctctat gggacgtccg atatcctgat cacggaggaa gaataccctg ttgatcgccg
      121 gagtaaaacg ccgtatccgc aattctccgt caagaaagat agtgccgatg tcggtgcagc
      181 tcatcagatt ctgcagatcg ttgttgaggt cgatcaactg ctcgttcttt tcctggtact
      241 ccgcgttcag gctgtgcatc tcctcgttga ccgactgcag ctcttcgtta ctgctctgga
      301 gctcctcgtt ggatgcaagc agctcctcgt tggtcgcctg aagctcttcg ttgctcgtct
      361 caagctcctc gacgatcgtc tgaaggttct ctctggcctc cgagagcgag cgttccagct
      421 cttcgatgcg caccttcgcg gcttcgtcga cggagaatga ctccgccttc gcggcaagcg
      481 agggcttcat ctcctccagg cgaatgaaaa agaagagatt gccctcctca gaccggctgt
      541 ccgtccgctc ttcgagcggc tccacggaga cctccacagt gttcagcgga gcgcctggaa
      601 gcgggtggga aacatccgaa aagcggacgg gggtcctgtt tttccgggaa cgctcgatcg
      661 ccgtcatgac ggcgacgcgc aggttcccgt gcaaaatgct gccgatatcc gtcctcacag
      721 ggccgatgat ctgcagatat tttcccgccc cgccgaaagt gtggagcaaa tcgcccttcg
      781 agttcacgag aaaacccgtc ggcatgcagc gctcaagaag aagcggatac agcgaagaaa
      841 gcggcatttt cgaacttccc cgttcggaac cggcagtcgc ctttttcttt tccggaagga
      901 gggagtagag cggccgggaa gtaatcgtcc cctgcttccg gtagatcttc agacggctgt
      961 ccactgtcaa aaactcgctc tgcgtgtcga ggacgctctc gctggagccg aggaagagaa
     1021 tgccttgctg ctcc
//
LOCUS       DAOSWW010001092         1031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_142830_length_1031_cov_1.998975, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001092 DAOSWW010000000
VERSION     DAOSWW010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142830_length_1031_cov_1.998975"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /gene="uxuA"
                     /locus_tag="PK442_15690"
     CDS             <1..556
                     /gene="uxuA"
                     /locus_tag="PK442_15690"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HOO89149.1"
                     /translation="GYKELSEEGLWENLKYFLEKTVPVAEESNVKMAIHPDDPPWSIF
                     GLPRIIRDEASYDRFVKLADSPNNGFSLCSGSLGARPDNDLPAMIRKFGGMGRIHFAH
                     VRNVKITGKGAFHETAHTTESGSLNMFEIMKAYADTGFTGPMRPDHGRMIWGETGKPG
                     YGLYDRALGAMYLAGLWEALQQRK"
     gene            780..>1031
                     /locus_tag="PK442_15695"
     CDS             780..>1031
                     /locus_tag="PK442_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89150.1"
                     /translation="MRKLFFLCMCSLFLFVGSLCVAEAAKPPLLAELFMASGVNPDGS
                     PGERVTAYPPEAPLLAVFAECRNVSAGMKVRFVWYFAPPG"
BASE COUNT          204 a          285 c          318 g          224 t
ORIGIN      
        1 cggatacaag gagctcagcg aggaggggct ctgggagaac ctgaagtact tcctggaaaa
       61 gaccgtacct gttgcggagg agtccaacgt caagatggcg atccacccgg acgacccccc
      121 ctggtcgatc ttcggcctgc cgcgtatcat tcgggatgag gcgtcctacg accggtttgt
      181 caaactcgcc gacagtccga acaacggttt cagcctctgc tccggatcgc tcggcgcgcg
      241 tcccgacaac gaccttccgg ccatgatccg caagttcggc ggaatggggc gcattcactt
      301 tgcgcacgtg cggaacgtga agatcacagg caagggggcg ttccacgaaa cggcgcacac
      361 gacggagtcc ggcagtctga acatgttcga gataatgaaa gcgtatgcgg acaccggatt
      421 caccggtccg atgcgtcccg accacggccg catgatctgg ggcgaaaccg gcaagccggg
      481 gtacggactc tacgaccgcg cgctcggcgc gatgtacctc gccggcctct gggaggcgct
      541 gcagcagagg aagtaacaaa gacgcttcac ggcgtgagcg cttcggggca tagtgtttcc
      601 tcaagtgaaa agaaggcgga atgaacgcgc atccagtatg cgctcattcc gccttcttgt
      661 tttcagcgag aagtgttata attcacaaaa ataccccgtc agtctttctt ctcctgtgac
      721 ggcgcgtaga ggcggttcgt tcggatggtc tgtgcatgcg tcaaaaagga ggaatccccg
      781 tgagaaaact cttcttcctg tgtatgtgtt cgctgtttct ttttgtcggt tctttgtgcg
      841 ttgccgaggc ggcgaaacct ccgttgctcg cggagctttt tatggcgtcc ggagtgaatc
      901 ccgatggctc gccaggtgaa agggtgacgg cttaccctcc cgaggcgcct cttttggctg
      961 tctttgccga atgcagaaat gtgtcggcgg ggatgaaggt gcgtttcgtc tggtactttg
     1021 ctccgccggg g
//
LOCUS       DAOSWW010001093         1030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_142954_length_1030_cov_3.290256, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001093 DAOSWW010000000
VERSION     DAOSWW010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142954_length_1030_cov_3.290256"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK442_15700"
     CDS             <1..252
                     /locus_tag="PK442_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOO89151.1"
                     /translation="RVNGKRVDIPSYLVESGDLVAVMEKSREVTLIKENAEVATARKA
                     PEWLEVNAEKIEGKVLSLPSREQIDAPVTEQLVVEFYAR"
     gene            276..>1030
                     /gene="rpoA"
                     /locus_tag="PK442_15705"
     CDS             276..>1030
                     /gene="rpoA"
                     /locus_tag="PK442_15705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOO89152.1"
                     /translation="MDYLRPEIRIEESTPKYAKVLIEPLERGYGATLGNSLRRVLLSS
                     IKGAAITSVRIDGVLHEFSTIPGVLEDVIEILLNLKHIPVRSFSQEVRVLRLETEGGG
                     LVTAANIQPDSEVEFVDPEAPICTMEEGARLSMDIYVEQGAGYASMERPRPAYLPVDA
                     LLVDAIFSPVLKVNYDVEAARVGQRTDFERLVLEIWTNGVHHPENTLCEASKILRGYF
                     GNIVEDMEKLNPVEPREQEAAAAGEGGVSEAAQ"
BASE COUNT          230 a          279 c          325 g          196 t
ORIGIN      
        1 cgtgtgaacg ggaagcgtgt ggacatcccc agctatctcg tcgaaagcgg cgatcttgtc
       61 gccgtgatgg aaaagagccg cgaagtcacg ctgatcaagg aaaatgcgga agtagcgacc
      121 gcgcggaagg cgcccgagtg gcttgaggtc aacgcagaaa aaatcgaagg gaaggttctt
      181 tcgctcccca gccgtgagca gatcgatgcg ccggtgaccg agcagctcgt cgtcgagttc
      241 tatgccagat agtggcgtga aaggtgttgg aagccttgga ttacttgcgg cctgagatcc
      301 gtatcgagga aagcacccct aagtatgcga aggttctcat tgaaccgctc gagcggggat
      361 atggagcgac tctgggaaat tctctcagaa gagttctact ctcctcgatc aagggtgcgg
      421 ccatcacgtc cgttcgaatc gacggcgtgc tccatgaatt cagcaccata cccggtgttc
      481 tggaagatgt catcgaaatt ttgctgaatc tgaagcatat acccgtccgc tctttcagcc
      541 aggaagtccg agtgcttcgg ctggagacgg aaggcggagg tcttgtcacc gccgcgaaca
      601 tacagccgga cagcgaagtg gagtttgtcg acccggaagc cccgatctgc acgatggagg
      661 agggcgcccg tctctcgatg gatatctacg ttgaacaggg cgccgggtac gcctccatgg
      721 agcgtccgcg cccggcctat ctccctgtgg acgccctgtt ggtcgacgcg atcttctccc
      781 ctgtcctcaa agtaaactac gacgtggagg cggcgcgcgt cggccagcgc acggactttg
      841 aacgccttgt ccttgagatc tggacgaacg gggtgcatca tcccgagaat acgctctgcg
      901 aagcgtcgaa aatcctgaga gggtacttcg gcaatatcgt ggaggacatg gagaaactca
      961 accccgtgga acccagggag caggaggcgg cagcagccgg cgagggcggc gtctcagagg
     1021 cagcccagga
//
LOCUS       DAOSWW010001094         1029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_143201_length_1029_cov_3.291581, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001094 DAOSWW010000000
VERSION     DAOSWW010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143201_length_1029_cov_3.291581"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PK442_15710"
     CDS             complement(<1..724)
                     /locus_tag="PK442_15710"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOO89153.1"
                     /translation="MESKKKILWYSFLIAIFILPLSGCSLSGRTNSYEEARALSEEAY
                     IFAYPMLENYRTMQVQAITSGNFNRFVHSTELLGPEDINALRPHNDTLSSTAWLDLRA
                     EPMVLTVPPLPDRYYSFQFVDLYTSNFAYVGARTIGSSGGVFLIAGPEWNGKTPRTVD
                     ALFQSEGNFVLCFGRTALNGPDDLPNVRAIQQEYTLEPLSKFLGTPPPEATPSEVFPL
                     YVKIAADSVHFISYLNFLMGQAK"
     gene            complement(845..>1029)
                     /locus_tag="PK442_15715"
     CDS             complement(845..>1029)
                     /locus_tag="PK442_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89154.1"
                     /translation="NPEVTPFVINRHAVKENSPALQEATPGSSCLSAKGEGASERKGA
                     FGCFFFPGKTPVKIEK"
BASE COUNT          258 a          271 c          281 g          219 t
ORIGIN      
        1 tcttcgcctg ccccatcagg aagttcagat aggagatgaa gtgaacagaa tccgcggcga
       61 tcttcacgta cagcggaaag acttccgaag gagtcgcctc ggggggcgga gtcccgagaa
      121 acttactcag aggctcaagc gtgtactcct gctgaatcgc acgtacattc ggcaggtcat
      181 ccgggccgtt gagagccgta cggccgaaac agagtacaaa gttcccttcg gactggaaga
      241 gagcatcgac agtacgaggc gtcttcccgt tccactcggg accggcgatc aggaaaaccc
      301 ctccagacga accgatagtc ctggccccga cgtaggcgaa gttgctggta tagaggtcga
      361 cgaactggaa cgaataatag cggtccggga gaggcggcac cgtgagcacc atgggctcgg
      421 cccgaagatc gagccaggcg gtcgaggaaa gcgtgtcgtt atgaggccga agcgcattaa
      481 tatcctccgg gccaagaagt tccgtagagt gaacgaaccg gttgaaattg cccgaagtga
      541 tggcctgcac ctgcatcgtt ctgtagtttt ccagcatagg ataggcgaag atatacgcct
      601 cctccgaaag agcgcgcgcc tcttcgtagc tgttcgtccg gcccgaaaga gaacacccgg
      661 agagcggcag gatgaaaatc gctatcaaga acgaatacca gagtattttc ttcttgctct
      721 ccacggccaa tgctcccttc acgtcaagat gggcacggta aactacgcgc cttccgatcc
      781 gtaaaattac gtacctggac gtattgtacc agaaaaagtg tcgaatggga aagggggaaa
      841 ttttttattt ttctattttt acaggagttt ttcctggaaa gaaaaagcag ccaaacgctc
      901 cttttctttc tgacgcccct tcacctttcg ccgaaagaca agaactcccc ggcgttgctt
      961 cttgcaacgc cggggagttc tctttaaccg catgacggtt gattacgaac ggtgttactt
     1021 caggattcc
//
LOCUS       DAOSWW010001095         1029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_143224_length_1029_cov_2.935318, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001095 DAOSWW010000000
VERSION     DAOSWW010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143224_length_1029_cov_2.935318"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PK442_15720"
     CDS             complement(<1..214)
                     /locus_tag="PK442_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89155.1"
                     /translation="MKTPVETRKNRVWDVEEALWKQGASLHLPVDGAARCWVCRAPQG
                     PVVAVFPDAKQAGTFLADWKALFPEEP"
     gene            complement(291..857)
                     /gene="pth"
                     /locus_tag="PK442_15725"
     CDS             complement(291..857)
                     /gene="pth"
                     /locus_tag="PK442_15725"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOO89156.1"
                     /translation="MKIVAGLGNPGPEYVFSRHNAGWLAVDHLVNRFSCGTPRMQFSS
                     MAWSFFMNGEKILILKPLTYMNLSGKAVGEAVDYLSADWEDVLVVYDDVALPFGKLRM
                     RSKGSAGGHKGLLSILGRAGTLEVPRLRIGVGAAPDARGIVSWVLGNFDKTEQNELPS
                     LLDSTAEAVEEWLSVGTEKAMNRVNATH"
BASE COUNT          203 a          325 c          281 g          220 t
ORIGIN      
        1 caggctcttc gggaaagaga gctttccagt cggccagaaa tgttccggcc tgtttcgcat
       61 ccggaaacac agcgacaaca ggcccttgag gcgcccgaca gacccagcat ctggcggcgc
      121 cgtcgacggg aagatgaagg gaggcgcctt gcttccacag cgcttcctca acgtcccata
      181 ctcggttttt tcttgtttct acaggggttt tcatacgttc gactccggaa tccgctccgc
      241 tccgggagac tgtgcgcaaa gaggtggcgg acttctccgc cacctctttg tcaatgagtt
      301 gcgttcaccc ggttcatcgc cttctcggtt ccaacggaga gccattcttc aacggcctcc
      361 gcagtgctgt cgagaagaga gggaagttcg ttctgttctg ttttatcgaa gttgccgagc
      421 acccaggaga caatcccgcg ggcatccggc gcggcgccga ctccgatacg cagcctgggc
      481 acttcgagcg ttcccgcccg gccgagaatg gagagcagcc ctttgtgtcc tccagcgctt
      541 cccttggagc gcatccggag cttcccgaag gggagcgcga cgtcgtcata cacgacaagg
      601 acgtcctccc aatcggctga aaggtagtcc acagcctctc cgaccgcttt tccgctgagg
      661 ttcatgtagg tgagcggttt gagaatcagg atcttctccc cgttcataaa aaacgaccat
      721 gccatcgaag agaactgcat cctcggcgtt ccgcaggaaa aacggttcac cagatggtcc
      781 accgcgagcc accccgcgtt atgacgggag aagacgtact ccggcccggg atttcccaaa
      841 ccagcaacga ttttcaaccg gcgttattcc tcgtctgcgc tcttcgcctt gcccttggcg
      901 acgacttcga cttccttctg ctcttcttcc gcttcggcct cggcgacgcc gcgaggaata
      961 atgatggcaa ccgccacatc atccttgtcg acaagcagcg tcgcatcgga ggggagagca
     1021 agatcgcgt
//
LOCUS       DAOSWW010001096         1023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_144612_length_1023_cov_3.700413, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001096 DAOSWW010000000
VERSION     DAOSWW010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144612_length_1023_cov_3.700413"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PK442_15730"
     CDS             complement(<1..1004)
                     /locus_tag="PK442_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89157.1"
                     /translation="MAKRYYGLENFEAPRPKVVEYYFEGIRDLYRDADWFAFNVNHAE
                     SREMDVPVGTYFKRDQVAPGMPEYVALHEANHAMQDACYLTGDLHYYIPWFDEGMADA
                     FGRIMLFRATHDEELMAKVKNFRTEVDVTDSRKVTYHYGEETAAMLLMRGRLPFVKAL
                     MQARRRDPFAIDWNSFARKIKSGWDPHIAVVTACNKNPDQFRKKLERDETSYRKDADL
                     DQMDLRVLGMFLSTNPPATLPAEEYRAALWFADEVAKRPCPHRVDPHAVPSALRGKLS
                     AWSADAAIPWTEIPEELRKKAPQLDVKVLIREDDVPEGMRGAAAKLASSYFILKREIG
                     "
BASE COUNT          160 a          338 c          316 g          209 t
ORIGIN      
        1 ctgcctatct cgcgcttgag gatgaagtag ctcgaggcga gcttcgcggc ggcgccccgc
       61 atcccttccg gtacgtcatc ctcccggatg agcaccttca catcgagctg cggtgccttc
      121 tttcgcagct cctccggaat ttcagtccac gggatagcgg catcggcgct ccacgcggaa
      181 agtttgccgc gcagcgcact tggcaccgca tgcgggtcta cgcggtgcgg gcaggggcgc
      241 tttgcgacct cgtcggcgaa ccagagcgcc gcccggtatt cctcggcggg gagcgtggcg
      301 gggggattcg tcgagaggaa catacccagg acgcgcagat ccatctgatc gaggtccgca
      361 tccttcctgt aggaggtctc gtcccgctcg agtttcttcc tgaactggtc gggattctta
      421 ttgcacgcgg tgacgaccgc tatgtgagga tcccagccgc tctttatctt cctcgcgaac
      481 gaattccagt ctatcgcgaa ggggtcccgc cggcgcgcct gcatcagggc tttcacgaag
      541 ggcagcctgc cgcgcatcag gagcatcgcg gcggtctcct ccccgtagtg gtaggtgacc
      601 ttgcgcgaat cggtcacgtc cacctcggtg cggaagttct tcaccttcgc catcagctcc
      661 tcgtcgtgcg tcgcacggaa gagcattatc ctgccgaacg cgtccgccat cccctcgtcg
      721 aaccacggga tgtagtagtg cagatccccc gtgaggtagc acgcatcctg catcgcgtgg
      781 ttcgcctcgt gcagcgccac gtactccggc atccctgggg cgacctggtc tctcttgaag
      841 taggtgccga cgggaacgtc catctcgcgg gactcggcgt ggttcacgtt gaaggcgaac
      901 cagtcggcgt cgcggtagag gtcgcggatc ccctcgaagt agtactcgac gaccttcggg
      961 cgcggcgcct cgaagttttc cagcccgtag tagcgcttcg ccatcgcggg cacggcctcc
     1021 atc
//
LOCUS       DAOSWW010001097         1023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_144652_length_1023_cov_2.837810, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001097 DAOSWW010000000
VERSION     DAOSWW010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144652_length_1023_cov_2.837810"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PK442_15735"
     CDS             complement(<1..437)
                     /locus_tag="PK442_15735"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO89158.1"
                     /translation="MNRRMSMMLLLFGLSLAVIFLGRSAWASGKPLLTLEKALEMADR
                     NNPLISASGERITQARARLDQASAASLPQLGVSLLYQEVQNEPRYPVVPAGYAKAGFE
                     STWKAALTLNWLLYSGGAVRLNTESKKVALSGVEAQEKRTRQAV"
     gene            complement(427..>1023)
                     /locus_tag="PK442_15740"
     CDS             complement(427..>1023)
                     /locus_tag="PK442_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO89159.1"
                     /translation="KRDARKSILCAAREGFALKGYYGTSMDSIVKTTGLSKGAIYWHF
                     PGKWEMYTAVLSEEAERIKGIVLPAEMPARSLQPTEFFMSRGERLIDMLADDALCRLL
                     FVHLMLEAMRGSAEMVEFVASLRSSITEDVLVVFEQLFPGDSLQMHALSHRDLVSMFV
                     SILHGLIMNLELTVSRDEAKKSWRFLIGCVFGGISHES"
BASE COUNT          222 a          334 c          261 g          206 t
ORIGIN      
        1 acagcctggc gcgtacgctt ctcttgcgcc tccactccgg agagggctac ttttttcgac
       61 tccgtattca gccggacggc tcccccgctg tagagcagcc agttcaacgt gagcgccgcc
      121 ttccaggtgc tctcgaaacc cgccttcgcg taccctgccg gaacaacggg gtagcgaggt
      181 tcgttctgca cttcctgata gagaagcgac acgccgagct gcggcagcga cgccgccgac
      241 gcctggtcca gccgcgcacg cgcctgcgtg attctctccc cggacgcgga gatgagcggg
      301 ttgttgcggt ccgccatttc aagcgctttt tccagggtca gcaacggctt ccccgacgcc
      361 cacgcggaac gtccgagaaa gatcaccgcg agagaaagcc cgaagagcag gagcatcatc
      421 gacatcctac gattcatgag aaatccctcc aaagacgcat ccgataagaa aacgccaact
      481 tttcttcgcc tcgtccctgc tcaccgtcag ttcaaggttc atgatcagcc cgtgaagaat
      541 actcacgaac atactcacca gatctctgtg cgaaagtgcg tgcatctgca gagaatcgcc
      601 cggaaagagc tgttcaaaga cgacgagtac atcttcggtg atggagctcc gaagggacgc
      661 gacgaactcg accatctccg cacttccccg catcgcttcg agcatgaggt gcacgaagag
      721 caaacggcag agggcgtcgt cggcaagcat atcgatgagg cgctctcctc tcgacatgaa
      781 aaactccgtc ggttgcagag acctcgccgg catttccgca ggaagaacaa tccctttgat
      841 tcgctccgct tcctccgaaa gcaccgccgt gtacatctcc cattttcccg ggaagtgcca
      901 gtagatcgct cccttgctca atcccgttgt tttgacgata gaatccatac tcgttccata
      961 gtaccccttc agggcaaatc cttccctggc ggcgcacaga atgctcttgc gcgcatcccg
     1021 ctt
//
LOCUS       DAOSWW010001098         1022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_144885_length_1022_cov_3.499483, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001098 DAOSWW010000000
VERSION     DAOSWW010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144885_length_1022_cov_3.499483"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1022)
                     /locus_tag="PK442_15745"
     CDS             complement(<1..>1022)
                     /locus_tag="PK442_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOO89160.1"
                     /translation="AAGLLERMAEPERAISFRVTWVDDAGKVNVNRGYRVQFNGAIGP
                     YKGGLRFHPSVNLSIMKFLGFEQTFKNSLTSLPIGGAKGGSDFDPRGKSEGEVMRFCQ
                     AFMTELYRHIGPDIDVPAGDIGVGGREIGYLYGQYRRVKGAFENGVLTGKGMSFGGSL
                     IRPEATGYGAVYYVNEVFRHEGDTIQGKTFALSGFGNVSWGVAKKLEELGAAAVTLSG
                     PDGYIYDPKGITGKKIEYMLTMRGSGRDKVQDYADEFGVEFHAGEKPWGVKADVYMPC
                     ATQNDVNMESAKKIAASGSKYYIEVANMPTTNDALNYLQQQKHLIVAPSKAVNAGGVA
                     TSALEM"
BASE COUNT          153 a          400 c          238 g          231 t
ORIGIN      
        1 tcatctccag cgcgctcgtc gccacgccgc ccgcgttcac cgccttgctc ggcgccacga
       61 tcaggtgttt ctgctgctgc aggtagttca gcgcgtcgtt cgtcgtcggc atgttcgcca
      121 cttctatgta gtacttcgac ccgcttgccg cgatcttctt cgcgctctcc atgttcacgt
      181 cgttctgcgt cgcgcacggc atgtacacgt ccgcctttac gccccacggc ttctcgcccg
      241 cgtggaactc cacgccgaat tcgtccgcgt agtcctgcac cttgtcccgg ccgctgcccc
      301 gcatcgtcag catgtattcg atctttttgc ccgtgatccc cttcggatca tagatgtatc
      361 cgtccggccc cgacagcgtc accgccgccg cgcccagctc ctccagcttc ttcgccacgc
      421 cccaagatac gttcccgaat cccgacagcg cgaacgtctt gccttggatc gtgtcgcctt
      481 cgtgtctgaa cacctcgttg acatagtaca ccgcgccgta gcccgtcgcc tccggccgga
      541 tcaggcttcc gccgaagctc atgccttttc ccgtcagcac cccgttctcg aacgcgccct
      601 tcacccggcg gtactgtccg tacagatacc cgatctcgcg ccctccgact ccgatgtccc
      661 ctgccggcac gtcgatatcc ggcccgatat gccggtacag ctccgtcatg aacgcctggc
      721 agaaccgcat gacctccccc tcgctcttcc cgcgcgggtc aaagtcgctg cctcccttcg
      781 ctccgccgat cggcaggctc gtcaggctgt tcttgaacgt ctgctcgaat cctaaaaact
      841 tcatgatcga aagatttacg ctcggatgaa accgcagtcc ccctttgtac ggcccgatcg
      901 ccccgttgaa ctgcacccgg tatccacggt ttacgttcac cttccctgcg tcgtctaccc
      961 acgtcacacg aaacgagatc gcccgctccg gctccgccat ccgctctaaa agcccagccg
     1021 ct
//
LOCUS       DAOSWW010001099         1021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_145216_length_1021_cov_2.291925, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001099 DAOSWW010000000
VERSION     DAOSWW010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145216_length_1021_cov_2.291925"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PK442_15750"
     CDS             complement(<1..854)
                     /locus_tag="PK442_15750"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047690.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOO89161.1"
                     /translation="MARNITSRTEDYSQWYLDIIKVAELADYAPVRGCMVIRPTGYAL
                     WESIQRHFDDAFKETGHVNAYFPLLIPNSFLEKEAEHVEGFAPECAVVTHAGGEELEE
                     PLIVRPTSETVIGHMYSKWIQSWRDLPILINQWANVMRWEKRPRLFLRTSEFLWQEGH
                     TAHATREEAMEETLKMLEVYRRIMTDILALPVIPGEKTEGERFPGADNTYTCETMMSD
                     KKALQAGTSHFLGQNFAKAFEIQFQNQDGNLEYAWTTSWGASTRLIGALIMTHSDDDG
                     LILPPRVAP"
BASE COUNT          195 a          296 c          285 g          245 t
ORIGIN      
        1 ggagctacgc gcggcggcag aatgaggccg tcatcatctg aatgcgtcat gatgagcgcg
       61 ccgataagac gtgtagaggc cccccaactc gtcgtccagg cgtattcaag attgccgtcc
      121 tggttttgga actggatttc gaaggccttg gcgaaatttt gcccgaggaa gtggcttgtt
      181 cctgcctgaa gcgccttttt atcgctcatc atggtctcgc aagtataggt attgtcggcg
      241 ccggggaagc gctccccctc tgttttctct ccgggaatca ccggcagcgc gaggatgtcc
      301 gtcatgattc tgcggtagac ttcgagcatt ttcagcgtct cttccattgc ctcttccctg
      361 gtggcgtggg cggtatgccc ctcctgccag agaaactcgg acgtacgaag gaagagccgg
      421 ggacgcttct cccagcgcat cacgttcgcc cactggttga tgaggatggg aaggtcgcgc
      481 cacgactgga tccacttgct gtacatgtga ccgatcaccg tttcggacgt cggacggacg
      541 atcaggggct cctcaagctc ttcgcccccc gcatgcgtga ccacagcgca ctccggggcg
      601 aacccttcca catgctccgc ctctttctca aggaatgaat tcgggatcaa aagggggaag
      661 taggcgttca cgtgccccgt ctctttaaac gcgtcgtcga aatgacgctg gatggattcc
      721 cagagcgcgt accccgtagg gcgtataacc atgcaccctc tgacaggcgc gtagtccgcc
      781 agctcggcga ccttgatgat gtcgagatac cactgggaat agtcttccgt tctcgatgtt
      841 atgttccgtg ccatttctct tcctcctgtt ttccgtatcc cccgtttcgg ggagtatcaa
      901 gcgctgtaca gcgcgttcac agttatgccc ggaaatatca gggcagcggg tatttccccc
      961 agatgggact gccgaggaag aaccctattt tcaagtcgct ttcgtcattg tagatgaaca
     1021 c
//
LOCUS       DAOSWW010001100         1019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_145655_length_1019_cov_2.891079, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001100 DAOSWW010000000
VERSION     DAOSWW010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145655_length_1019_cov_2.891079"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PK442_15755"
     CDS             <1..692
                     /locus_tag="PK442_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89162.1"
                     /translation="FRLERPQAGAMFCCGFFTNIGAIGGLVTFVFLGEAGYAYVPLFK
                     LFEDMVYYGVGFPVARLYSDTKTTGGVSLAARLTRDPFLLVSIFSLTTGTTLNLLNVP
                     RPEVFSQLNALLIPLTTVILLASIGMAMRFGKTSAYLKEGVAILGVKFLLVPAVCTSL
                     AALLGLGGIAGGLPLKVVLVLSSMPVAFNALVPPSLYALDLDMANSCWLISVLGMAGL
                     LPLLHVLLTLL"
     gene            824..>1019
                     /locus_tag="PK442_15760"
     CDS             824..>1019
                     /locus_tag="PK442_15760"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOO89163.1"
                     /translation="MRGIGVSPGIAIGKAVIHWKEQIDIVREYVDEPERELRRFRTAL
                     EFAGDQIRESYSRVLQNVGPN"
BASE COUNT          173 a          321 c          299 g          226 t
ORIGIN      
        1 tcttccggct ggaacgaccg caggcgggag cgatgttctg ctgcggattt ttcacaaaca
       61 tcggggccat cggcgggctg gtgaccttcg tctttctcgg cgaggcgggc tatgcctacg
      121 tcccgctctt caagctgttc gaggacatgg tctattacgg cgtcggcttt cccgtagcca
      181 ggctctacag cgatacgaag acgacgggcg gcgtgagcct cgcggcacga ctgacgcgcg
      241 atccgttcct tctcgtctcc atcttcagtc tgacgacggg aacaacgctg aatctgctca
      301 atgtgccgcg ccccgaggtc ttctcgcagc tcaacgcgct gctcattccg ctgactacgg
      361 tgatcctgct ggcctccatc ggcatggcca tgcgcttcgg caagacctcc gcctacctga
      421 aagaaggcgt cgccattctc ggcgtgaagt ttctgctggt tccggccgtc tgcacgtcgc
      481 tcgccgcctt gctggggctg ggcggcatcg ccggagggct tccgctcaag gtcgtgctcg
      541 tcctctcctc catgccggtg gcgttcaacg cgctggtgcc cccgtcgctc tacgcgctcg
      601 acctggacat ggcaaacagc tgctggctga tctccgtgct cggcatggcg gggctgctgc
      661 cgctccttca cgtgctgttg acgctcttgt aagcgcgtcg cttcccgcac ggcggatttc
      721 cattcttccg gaggacgaaa cgcttgttca ctcctttgcg cacaaaggcc gaactgcgtg
      781 tataattatg tgatacacat tcgacaggca ggtgctgaaa accatgagag gaatcggggt
      841 atctcccgga atcgccatcg gcaaagcggt cattcactgg aaagagcaga tcgacattgt
      901 tcgggagtac gtcgacgaac ccgagcggga acttcgccgt tttcgcaccg cgctcgaatt
      961 cgccggagat cagattcgcg aatcatattc gcgggtactg cagaacgtcg gccccaacg
//
LOCUS       DAOSWW010001101         1018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_146044_length_1018_cov_1.616822, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001101 DAOSWW010000000
VERSION     DAOSWW010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146044_length_1018_cov_1.616822"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1018)
                     /locus_tag="PK442_15765"
     CDS             complement(<1..>1018)
                     /locus_tag="PK442_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89164.1"
                     /translation="ELSRKINCSYWVDELNAIADTVGIDRELYIAFTFGRYRDLALLY
                     KGVGCTSFAITAPATKNGQIIFHKTRETAPDLQAGYLKRIVGVEGADRQPYKFFGEMG
                     TADTGVSFFVNEKGLAGAADVPVQWQNRECYVGAYKGDLPLVDPPKYDGFMNHYVPRY
                     IAEHCKDVDEAREALHSFVEKGYIASGKWGTNYLFVDARGKILQIADDCYKIVEEQID
                     PALKHGEKSYKGIYFTVHRENDYGTPEDALVAHYGNITLELANSPKVSKHPAMWNFAR
                     AQSSATILIDPEHPEILTTVFVTLPAYGYSIPFLMGANATPRVLVDGTVYSVQKESFR
                     YSEYY"
BASE COUNT          205 a          277 c          273 g          263 t
ORIGIN      
        1 gtagtattcg ctgtatctga agctctcctt ctgcacgctg tacaccgtac cgtcgacaag
       61 aactctgggc gtcgcgttcg ctcccatgag gaatggaatc gaataaccgt acgcaggcag
      121 cgtgacgaaa acggtcgtca ggatttcagg gtgttcgggg tcgatgagga tcgtcgcgct
      181 cgactgggcc cttgcgaaat tccacatcgc ggggtgtttc gataccttgg gcgagttcgc
      241 aagctcgagc gtgatatttc cgtaatgcgc gacaagcgcg tcttccggcg tcccataatc
      301 gttctcccgg tgtacggtga agtagatccc tttataactt ttctcgccgt gtttcagcgc
      361 cggatcgatc tgttcttcga cgatcttgta gcagtcgtcg gcgatttgaa ggattttccc
      421 cctcgcgtcc acgaacagat agttcgtacc ccacttcccg cttgcaatgt accctttttc
      481 aacgaacgaa tggagcgctt cccttgcttc gtcgacgtcc ttgcagtgtt cggcgatgta
      541 gcgcggaaca tagtgattca taaatccatc gtatttcggg ggatcaacaa gggggaggtc
      601 acctttatac gctcccacgt agcactctct gttctgccat tgaaccggca cgtcggccgc
      661 tccggcgagc cctttttcgt tgacgaaaaa cgaaactccg gtatcggcag ttcccatttc
      721 accgaaaaac ttataaggtt gcctgtcggc gccctcgact ccgacaatcc gtttcaagta
      781 gccggcctgg agatccggag cggtctcccg cgttttgtga aaaatgatct gtccgttttt
      841 cgtggcgggc gcggtgatcg cgaacgaagt gcatcccaca cctttataga gaagggcaag
      901 atccctgtat cttccgaagg taaaggcgat gtagagttcc ctgtctattc cgaccgtatc
      961 ggcgatggcg ttgagttcgt cgacccagta ggagcagtta attttccggg agagttcg
//
LOCUS       DAOSWW010001102         1016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_146435_length_1016_cov_2.437045, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001102 DAOSWW010000000
VERSION     DAOSWW010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146435_length_1016_cov_2.437045"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK442_15770"
     CDS             complement(<1..532)
                     /locus_tag="PK442_15770"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOO89165.1"
                     /translation="MKRVKLGSAVAEKTIKTKRVFAGRLLKCDVVSVALEKGKKSVRE
                     VVRHPGAAAALCRTVDGRFVFVKQFRKAVEKQVLEIVAGTLEPGENPSACARREVREE
                     TGYRVSALKKLGVVYPSPGYSDERLHVYLADLRKTRGETDHDHDERIKTVHISRAGVE
                     KKIASGSISDAKTLAAW"
     gene            complement(519..>1016)
                     /locus_tag="PK442_15775"
     CDS             complement(519..>1016)
                     /locus_tag="PK442_15775"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOO89166.1"
                     /translation="RCSNNFGPYQFPEKLIPLMIANAMEGKELPVYGDGLHVRDWIYV
                     LDHCSGVDLILRKAEAGSIYNISSAHDVPNIEVVKGILKILGKDESLIKYVEDRPGHD
                     RRYALDASKLRNDLGWKPSHSFENALKSTVEWYVTNREWWERIRSGEYMEYYSRMYGN
                     RGEKS"
BASE COUNT          225 a          319 c          197 g          275 t
ORIGIN      
        1 tccatgccgc gagcgtcttg gcatccgata ttgacccaga ggcaatcttc ttctcaaccc
       61 cggccctgga aatatgaact gtttttattc tctcatcgtg atcgtgatcc gtttcccctc
      121 ttgtctttcg cagatccgct aaataaacgt gcagtctttc gtccgaatat cccggggacg
      181 ggtacaccac ccccagcttc ttcagcgcgg acactctata tcccgtttcc tctcttactt
      241 cacgtctcgc gcaggcggag ggattctcgc ccggctcaag agtaccggcc actatttcga
      301 gaacctgctt ctctaccgct ttcctgaatt gttttacgaa aacaaatcgt ccatcgacag
      361 ttctgcaaag cgccgccgcc gcgccaggat gccgcaccac ctctcgaaca gacttttttc
      421 ccttctccaa cgcgacggaa acaacatcgc atttcagaag cctcccggca aaaacccgtt
      481 tggtttttat ggttttttcc gccactgctg aaccaagctt aactcttttc accccgatta
      541 ccgtacattc tactgtagta ctccatgtac tcaccactgc gtatgcgctc ccaccattca
      601 cggttcgtca cgtaccattc aactgtcgac ttaagcgcgt tctcaaaaga atgagacggt
      661 ttccagccaa gatcgtttct caacttcgaa gcgtcaagcg cgtacctgcg atcgtgaccc
      721 ggtctgtcct caacatattt aatcagcgat tcatctttcc cgagtatttt gagaataccc
      781 ttcaccactt ctatgttcgg aacatcatga gcactgctga tgttgtaaat gcttcctgcc
      841 tcagctttcc tcaggataag atccactccg gaacagtggt ccagcacgta tatccagtct
      901 ctcacatgga gcccgtcccc gtaaaccggc agttccttcc cctccatcgc gttggcgatc
      961 atcagaggaa tcagtttctc cggaaactga tacggaccga aattatttga acagcg
//
LOCUS       DAOSWW010001103         1014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_146848_length_1014_cov_3.890511, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001103 DAOSWW010000000
VERSION     DAOSWW010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146848_length_1014_cov_3.890511"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1014
                     /locus_tag="PK442_15780"
     CDS             <1..>1014
                     /locus_tag="PK442_15780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO89167.1"
                     /translation="LLGLVVLLALNVLRRLRVEKMIVSINRNLEERVRDRTQELDRAL
                     LRAESANIAKSEFLANMSHEIRTPMNGVIGMTDLLLETPLTLEQRRYALMLQQSAESL
                     LELLNDILDFSKIEAGKLDLELIDFDLHDLLGDIASAQAVRASEKGVEVLCAIDPDVP
                     SSLFGDPGRLRQIVNNLAGNAVKFTAEGEIEIGVSLTSEDEESATLAFRVRDTGIGIP
                     EEKRDMLFKKFSQLDSSTTREYGGSGLGLAISKQLVGMMGGEIGVESPAPSAGETSGG
                     PGSLFHFTVRLKKSTATRTTARPPAELQDIRILVVDDNATNREILAAYIAAQGIRSAE
                     AANG"
BASE COUNT          216 a          308 c          316 g          174 t
ORIGIN      
        1 cctgctgggg ctcgtcgtgc tgctggcgct caatgtcctg cggcgtctgc gcgtcgagaa
       61 gatgatcgtc tccatcaaca ggaatctgga agagagagtc cgcgaccgga cgcaggaact
      121 cgaccgcgcc cttctgagag cggagagcgc caacatcgcg aagagcgaat ttcttgccaa
      181 catgagccac gagatacgaa cgccgatgaa cggcgtcatc ggcatgaccg atctgcttct
      241 ggagactccg ctgacgctgg aacagcgccg ctacgccctc atgctgcaac agagcgcgga
      301 gtcgcttctg gagctgctga acgatattct ggatttttcg aagatcgaag cgggaaaact
      361 cgatctggag ctgatcgact tcgatctgca cgatcttctg ggcgatatcg cctccgccca
      421 ggctgtgcgc gcatcggaaa aaggggtgga agtgctctgc gcgatcgacc ccgacgttcc
      481 atcctcgctc ttcggcgacc cgggacgtct ccggcagata gtgaacaacc tcgcggggaa
      541 tgcggtgaag ttcaccgccg aaggcgagat cgaaatcggc gtgtcgctga cgtccgagga
      601 cgaagagtcc gcgacgctcg cgttccgcgt ccgggatacg ggcatcggca tcccggagga
      661 gaagcgggac atgctcttca agaagttcag ccagctcgac tcgtcgacca cccgggagta
      721 cggaggcagc ggactcggac ttgccatatc gaagcaactg gtcgggatga tgggcggcga
      781 gatcggcgtc gaaagtccgg ccccctcggc aggagaaacc tccggaggcc ccggttctct
      841 ctttcatttc accgtacgcc tcaaaaaatc aaccgcgacg cgcacaacgg cacgtcctcc
      901 ggcggagctg caggatatca ggatcctggt tgtcgacgac aatgcgacga accgcgagat
      961 cctggccgca tacatcgccg cccaagggat acgctccgcc gaggcggcga acgg
//
LOCUS       DAOSWW010001104         1013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_147104_length_1013_cov_3.709812, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001104 DAOSWW010000000
VERSION     DAOSWW010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147104_length_1013_cov_3.709812"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /gene="nudC"
                     /locus_tag="PK442_15785"
     CDS             <1..478
                     /gene="nudC"
                     /locus_tag="PK442_15785"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOO89168.1"
                     /translation="HCPRCAALMQPAEEGRAYACPQCAYVSYPTLSPAIIVAVVRDGK
                     LLLAHGTRFPSGRYSVLAGFVEPGESLEETVEREIFEEVGIKVKDIAYFGSQPWPFPH
                     SLMLGFTARWASGEIVPDGVEIGDADWFTPENMPEIPPSISISRKLIDHWLSGGIA"
     gene            504..>1013
                     /locus_tag="PK442_15790"
     CDS             504..>1013
                     /locus_tag="PK442_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89169.1"
                     /translation="MIRALGILVFLAFALALHPNAEAEAKEIEKGLSRGSVLLSWFKD
                     AEMDFQLLRSLGADVAGGGAAGEIFVTAKGITDGNPASWSAAFLALGERLEADGNARL
                     ERGHKVSAMESLLRAASYYRAAEYYGDPSAPETAQTGMKCRDVFMKGMQLAAWKTESV
                     LIPFGKKKDF"
BASE COUNT          192 a          291 c          355 g          175 t
ORIGIN      
        1 ccactgcccc cgctgcgccg cgctcatgca gccggccgag gaggggcggg catacgcctg
       61 tccgcagtgc gcgtacgtct cctaccctac gctcagcccg gcgattatcg tggcggtcgt
      121 gcgcgacggc aagcttcttc tggcgcacgg aacccgcttt ccttcggggc gctacagcgt
      181 gctcgcgggg ttcgtcgagc cgggcgagag cctggaagag actgtcgaac gggagatctt
      241 cgaggaagtc ggcatcaagg tgaaggacat cgcctacttc ggcagtcagc cgtggccgtt
      301 tccccattcg ctgatgctcg gcttcaccgc gcgctgggca agcggggaga tcgttccgga
      361 cggagtcgag ataggggacg ccgactggtt cacacccgag aatatgccgg agattcctcc
      421 atccatcagc atttcgcgaa aactgatcga ccactggctc tccggaggta ttgcgtaaca
      481 cagtcaggga ggtggaacaa cggatgatac gagcgctcgg aatactggta ttccttgcct
      541 tcgccctcgc gttgcatccg aacgcggaag cggaggcgaa ggagatcgag aagggactgt
      601 cgcgcggcag cgtcctgctc tcatggttca aggacgcgga gatggacttt cagcttctcc
      661 gcagccttgg cgcggacgtt gcggggggcg gagcggcagg ggaaatcttc gtgaccgcga
      721 aggggataac ggacggtaat ccggcctcat ggtcggcggc ctttctcgcg ctgggagagc
      781 ggctggaagc ggacggaaac gcccggctgg agcgggggca caaggtcagc gcgatggaga
      841 gcctgctgcg cgccgcgtcg tactacaggg cggcggagta ctacggagac ccgtcggctc
      901 ccgagaccgc gcagacggga atgaagtgcc gcgacgtctt catgaagggg atgcagcttg
      961 cggcgtggaa gacggagagc gtgctcattc cgttcggcaa gaaaaaagac ttt
//
LOCUS       DAOSWW010001105         1012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_147408_length_1012_cov_2.907001, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001105 DAOSWW010000000
VERSION     DAOSWW010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147408_length_1012_cov_2.907001"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PK442_15795"
     CDS             complement(<1..594)
                     /locus_tag="PK442_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO89170.1"
                     /translation="MNSAIEEMELTPRRIVEYLDRYIVGQEKAKKSVAIALRNRIRRR
                     NLSPEMAKEVAPKNILMVGPTGVGKTEIARRLAELVKAPFVKVEATKFTEVGYVGRDV
                     ESIIRDLAEAAVAMVKKRKIEEVQTPAAERAEQRLADALLPGSEKKPSMPDFMKIFGG
                     GREREEAPQPAPEEERMRESTRRKLCTLLEEGKLDDRE"
     gene            complement(591..>1012)
                     /gene="hslV"
                     /locus_tag="PK442_15800"
     CDS             complement(591..>1012)
                     /gene="hslV"
                     /locus_tag="PK442_15800"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOO89171.1"
                     /translation="RRLYKGSVLAGFAGSTADAMTLLERFESRLEENSGDLMRSAVAM
                     VKEWRTDRALRRLEAMMIVADAKLSLLLSGAGDILEPENNIASIGSGSGFAQAAGMAL
                     LETSDWAPSKIAKRSIEIASEICIYTDSIVTVEAIGE"
BASE COUNT          166 a          344 c          273 g          229 t
ORIGIN      
        1 ctcgcggtcg tcgagcttcc cctcttcgag cagcgtgcac agctttctgc gggtggattc
       61 ccgcatgcgc tcctcctcgg gagcaggctg gggcgcctct tcgcgctccc gtccgccgcc
      121 gaagatcttc ataaagtcgg gcatggaggg tttcttctcc gatccaggaa gcagcgcgtc
      181 ggcgagacgc tgctccgccc gttccgccgc cggggtctgc acctcttcta tcttgcgttt
      241 cttgaccatg gccactgcgg cttcggcaag atctctgatg atggactcga cgtcgcggcc
      301 cacatacccc acttcggtaa actttgtcgc ctccaccttg acgaacggcg ctttcaccag
      361 ctccgcaagc ctgcgggcta tctcggtctt gccgacgccg gtaggcccca ccatcaggat
      421 attcttcggc gccacttctt tcgccatctc gggagagaga tttctcctcc ggatacggtt
      481 tcgcagcgca atggcgaccg acttctttgc tttctcctgg cccacgatgt accggtcgag
      541 atactcgacg atccggcgcg gcgtgagctc catttcttca atggcgctgt tcattctcct
      601 attgcctcca cagtcacaat gctgtctgta taaatgcaga tctccgaagc gatctcaata
      661 gagcgcttcg ctattttcga cggcgcccaa tcggacgttt cgagcagcgc cattccggcg
      721 gcctgcgcga accccgatcc ggagccgatt gaagcgatgt tgttctccgg ctcaaggata
      781 tcgccggctc cggaaagaag cagcgagagc ttcgcgtccg cgacgatcat catcgcttcc
      841 agcctgcgga gcgccctgtc ggtacgccac tccttgacca tcgccaccgc cgaacgcatc
      901 agatcgccgg aattctcctc caggcggctt tcaaaacgct ccaggagcgt catggcgtcg
      961 gcggtgctcc cggcgaatcc tgcgagcacg ctccctttgt agagtctgcg ga
//
LOCUS       DAOSWW010001106         1010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_147972_length_1010_cov_2.631414, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001106 DAOSWW010000000
VERSION     DAOSWW010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147972_length_1010_cov_2.631414"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /gene="carA"
                     /locus_tag="PK442_15805"
     CDS             <1..931
                     /gene="carA"
                     /locus_tag="PK442_15805"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOO89172.1"
                     /translation="DDPGLAEIKLRGLVVRSCFSGPVPRGRISLDAFLKKHGIPGLAD
                     VDTRSLTIRLREKGSCNSFIVAPSAGDALSVEEMRSVQSSFRRLPSMEGANLLASVGT
                     RTALPASFGNHPRFTVVDCGVKGNILRELGARGAAVTVLPWTASSEDVLSTTPDGVLF
                     SNGPGDPAALASVVTTIRNLIGVVPVFGICLGHQMIALALGGRTRKMKFGHHGCNHPV
                     RDERTGKVQVTSQNHGFEVDEQSLPPEVAVWFRNANDGSVEGLMHGRLPVMSCQFHPE
                     AAPGPRDSSWIFDEFVGIASRRKEGSSCLLETI"
BASE COUNT          170 a          305 c          327 g          208 t
ORIGIN      
        1 ggacgatccg ggattggcgg agataaagct gcgcgggctt gtggtgcgtt cgtgcttctc
       61 cggtcccgtt ccccgcggac gcatctccct cgacgcgttc ctcaaaaagc atggaattcc
      121 cggtcttgcc gatgtggaca cgcgctcatt gacgatccgc ctgcgtgaga aggggagctg
      181 caacagcttc atcgtcgctc cgtcagcggg agatgcgctg agcgtagagg agatgcgttc
      241 cgttcagtcg tcgttccgtc gacttccctc aatggaaggg gcgaatcttc tcgcatcggt
      301 gggtacgcga acggcgcttc ccgcttcttt cggtaatcac cctcgtttca ccgttgtcga
      361 ctgcggggtg aaggggaata tccttcgcga actcggggcg aggggcgccg cagtcacggt
      421 gcttccatgg accgcgtcgt cggaggacgt cctgtcgacc acccccgacg gggtgctctt
      481 ctcgaacggt cccggcgatc ctgccgcgct cgcgtcggtg gtgacaacca tccgaaatct
      541 catcggcgtc gttccggttt tcggtatctg ccttgggcat cagatgatcg ccctcgcgct
      601 cggaggccgg acgcggaaga tgaagttcgg ccatcacggc tgcaatcatc ccgtcaggga
      661 cgaacggacc ggcaaagtgc aagtgaccag ccagaatcac ggtttcgaag tggatgaaca
      721 gtcgcttccg ccggaggtgg cagtatggtt ccggaacgcc aacgacggct cagttgaggg
      781 gctgatgcac gggcgccttc cggtgatgtc gtgccagttc caccccgagg ccgctccggg
      841 gccccgcgat tcctcgtgga ttttcgacga gttcgtcggg atcgcctcca ggagaaagga
      901 gggatcctcg tgcctgctcg aaacgatctg aagaagatcc tcgttctcgg gtcgggcgcc
      961 atcgtcatcg ggcaggcgtg cgagttcgac tactccggga cccaggcctg
//
LOCUS       DAOSWW010001107         1009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_148245_length_1009_cov_2.481132, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001107 DAOSWW010000000
VERSION     DAOSWW010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148245_length_1009_cov_2.481132"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PK442_15810"
     CDS             complement(<1..658)
                     /locus_tag="PK442_15810"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO89173.1"
                     /translation="MNIPMGATMEMRFPLLQDFLPTILSCCREISGLFGFSHKDTLRL
                     EIALEEALTAIIPGESSDPLPKSLTVRFRRVPLGIRVILGEFHAPLDRKSMDAYSPGE
                     EPDERDFSGLGLFLMRNLVDQAAFYDFEAEGRGKELHLFKMLRREADEPLPQLPLAAI
                     PEKNEPAVREKPFSSERVAPSRVSAITSSFAHPGGESVESLSSIQLREALADPDISLF
                     A"
BASE COUNT          207 a          259 c          332 g          211 t
ORIGIN      
        1 cggcgaagag ggagatgtcg gggtccgcca gagcttcgcg gagttgaata gaagaaagcg
       61 attcgacgct ttcacccccg ggatgggcga aagaggaggt gatggcggag acgcgggagg
      121 gagcgactcg ttcggaggaa aagggttttt cgcgaacggc gggctcgttt ttttccggta
      181 tcgcagccag aggaagctgc gggagcggtt cgtcggcttc cctccgcagc attttaaaga
      241 ggtgcagctc tttccctctc ccttccgcct caaagtcgta gaaggccgcc tggtctacaa
      301 gattgcgcat cagaaagagg ccgaggccgg aaaaatcccg ctcatccggc tcttcccccg
      361 gggagtacgc gtccatgctc tttctgtcga gcggggcgtg aaactcgccg agaatgactc
      421 tgattccgag gggaactctc cgaaagcgca ccgtcaggga cttcgggagg gggtcggaag
      481 attctccggg gatgattgcc gtgagcgcct cttccagggc tatttcgagc cgcagcgtat
      541 ctttatgtga aaacccgaag aggccgctga tttcgcggca gcaggagagg atcgtcggca
      601 gaaagtcctg gaggagaggg aagcgcatct ccatcgtggc gcccattgga atgttcatcg
      661 cgcagctcct ttcaaaagag gcggtatcga gagcctatct gtaccgttcg gttcaagtgc
      721 tcagtatgtc atatcgaaaa gattctttca acacttcgta gttcgtgaaa acgcgggcag
      781 ggattgcgtc tggacgtccg cggaaaaaag cggtactctt gacggcgcgt ggtccctgcg
      841 aagagtgagg agggcgccgc aatccggagg gaggtcgttt cggtgaaaag aatacactca
      901 tttctccatc gctgcaaaaa aatgtggttc gggggcggag gcgcggcgtg cgcgctcgcg
      961 ttcctgtgtt gcgtcttctt ccccggcttg acgggcgcgc attccgcgt
//
LOCUS       DAOSWW010001108         1007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_148769_length_1007_cov_2.160714, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001108 DAOSWW010000000
VERSION     DAOSWW010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148769_length_1007_cov_2.160714"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>1007)
                     /locus_tag="PK442_15815"
     CDS             complement(71..>1007)
                     /locus_tag="PK442_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89174.1"
                     /translation="VAEDYPDCLEIVERLVKPERTRVDADGNFVLRKPLPQRWWHYAD
                     KRPALYSTIAPLKRVMVIAIGATKYPYFSLSKKEIVFSHALGVIALDAKACHALLNSD
                     FHVMWAWKNASTLESRMRYTPSDIFETFPFPPRLEPSSQGKSSSSFRLLDQLGERLHE
                     GRRALMTMLGIGLTSLYNLYHDPDLSDESVVKTAKYSGEEASRARKEIEGLRDLHVRI
                     DEAVRDAYGWSDIALDHGFHELEFLPENDRVRYTVSRAARGEILKRLLELNHQRHEEE
                     FAAGLVDKDGKKIKKKAGMKKKRTSAADQGQPLPF"
BASE COUNT          196 a          256 c          302 g          253 t
ORIGIN      
        1 cttggctctt ccgctgcgga gaaactcttc tcctcaggaa ttcttgcgaa cggcggcgcg
       61 tatttctcgg ttaaaatggg agcggctgcc cttggtcggc tgcggaggtg cgtttcttct
      121 tcattcctgc cttcttcttg attttctttc cgtctttgtc gacgaggccg gcggcgaatt
      181 cctcttcgtg gcgttgatgg ttcagctcca gcaggcgctt gagaatctct ccgcgggcgg
      241 cgcgggagac tgtgtagcgg acgcggtcgt tttcggggag gaattcgagt tcgtggaagc
      301 cgtggtcaag ggcgatgtcg ctccagccgt aggcgtcgcg gacggcttcg tcgatgcgga
      361 cgtgaaggtc gcggagtccc tctatctcct tgcgggcgcg ggacgcctcc tccccggagt
      421 acttggcggt ttttacgacg ctctcgtcgg agagatctgg gtcgtgatag aggttgtaga
      481 gggaggtgag accgatgccg agcatcgtca tgagcgcgcg ccttccctcg tgcaggcgtt
      541 ctcctagttg gtcgagcaac ctgaaggagg aggaagactt tccctgcgag gacggttcaa
      601 gcctcggagg gaagggaaag gtttcaaata tatcggaggg tgtgtagcgc attcttgatt
      661 ccaaagttga tgcgtttttc caagcccaca tcacgtgaaa atcgctgttg agaagagcat
      721 gacaggcttt agcatcaagc gcaataactc caagagcatg agaaaaaaca atctctttct
      781 ttgaaaggga gaagtatggg tattttgtcg ctcctatcgc aattaccata acccgcttca
      841 aaggagcgat ggtactgtag agtgccgggc gtttatctgc gtaatgccac catctttgcg
      901 gaagaggttt tctgagtacg aaatttccgt ccgcgtcaac acgagttctc tcaggcttca
      961 ccaaccgctc gacgatctcc agacagtccg ggtagtcctc cgcaacc
//
LOCUS       DAOSWW010001109         1005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_149176_length_1005_cov_3.473684, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001109 DAOSWW010000000
VERSION     DAOSWW010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149176_length_1005_cov_3.473684"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..1005
                     /locus_tag="PK442_15820"
     CDS             370..1005
                     /locus_tag="PK442_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO89175.1"
                     /translation="MSAYSDEELERMLDDLESDLVERKESFRGDVPKKARQAVCAFSN
                     DLPFDLHPVPEASLNDLSRVLFESEYLPAAFAPEILEANNRTYEERLASCRMVVSPDN
                     PVPTLVGILALGKRPRAFFPGASVQFLRIDGEEFADPVIDEENVDGPLREMLSKIGQK
                     LSAHNRVFVDIVSSPTEKRIYDYPPPALQQLLYNAVLHRTYEKTNTPVRIY"
BASE COUNT          223 a          295 c          277 g          210 t
ORIGIN      
        1 gcaaaccgcc agcgagccct cccgcggctt cctcgcgctc attcttcgcc catcgccaat
       61 aagttcgtag tatagtgagc gtacacgatt actcctttgc cgtccggcgt tgtagaattt
      121 tccgcagaag cagaaagata ctcaaagtgc aggtacgtcc ataataccac ggaacgggtc
      181 tcggctcttt caaggatatc cgaagactcc gaaaggcttc tcgagatggg cctttccgcc
      241 tcttcatcgt gtgtccgcac atcgaagcgt tcatgctatt ataaagaaac gggaaaaaga
      301 tattccagaa aacggaatcg ggcggcgaag cgtttcatcg ccctgaattc gctgaaaggt
      361 ggtgcggcga tgtcggccta ttcggatgag gagcttgagc ggatgctcga cgacctcgaa
      421 tccgatcttg ttgaacgaaa ggaatccttc cgcggggatg tgccgaaaaa agcgcggcag
      481 gctgtctgcg cattctccaa cgacctgccg ttcgatctcc accctgttcc ggaggcctcg
      541 ctgaacgatc tttccagagt gctcttcgag agcgagtatc ttcccgcggc cttcgcgccg
      601 gagattcttg aggcgaacaa cagaacgtac gaagagcggc tcgcgagctg ccggatggtc
      661 gtctcccccg acaatcccgt tcccacgctc gtcggcattc tcgccctggg aaagcgcccg
      721 cgcgccttct tccccggggc ctcggtgcag tttctgcgaa tagacggcga agagtttgcc
      781 gatccggtga tcgacgagga aaacgtcgac ggcccgctca gagaaatgct ttcgaagatc
      841 gggcagaaac tttccgcgca taaccgggtg ttcgtggata tcgtctcgtc ccctacggag
      901 aagagaatct acgactatcc gccgccggcg ctgcagcagc tgctgtacaa cgcggtgctc
      961 cacagaacgt atgagaagac gaacacgccg gtccgaatct actag
//
LOCUS       DAOSWW010001110         1001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_150191_length_1001_cov_3.886892, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001110 DAOSWW010000000
VERSION     DAOSWW010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150191_length_1001_cov_3.886892"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PK442_15825"
     CDS             complement(<1..721)
                     /locus_tag="PK442_15825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO89176.1"
                     /translation="MSLINVAHLTFAYEGGLENIFEDVCFRIDTDWKLGFTGRNGRGK
                     TTFLNLLLGRYEYEGTISSSVDFEYFPYDVPDEEMETLELIRSVAPGAEEWEIAREVS
                     LLDVADDALLRPFRTLSNGERTKVLLAALFLKENSFLLIDEPTNHLDMEARAAVTAYL
                     NRKKGFILVSHDRVFLDACIDHILSINRTNIEVQKGRFSSWWKNMQMREHYERSENER
                     LKKEIGRMQEAARRTSDWSDKV"
BASE COUNT          197 a          300 c          292 g          212 t
ORIGIN      
        1 cgaccttgtc cgaccagtcc gacgtccggc gggcggcctc ctgcatccgg ccgatctcct
       61 tcttcaggcg ctcgttctcc gaccgctcgt agtgctctcg catctgcatg tttttccacc
      121 aggatgagaa tctccccttc tgtacttcga tgttcgtcct gttgatcgag agaatgtggt
      181 cgatgcaggc gtcgagaaag accctgtcgt gcgagacgag gatgaagcct ttcttccggt
      241 tcaggtaggc ggtcacggcg gcgcgcgcct ccatgtccag gtggttggtg ggctcgtcga
      301 tcagcaggaa gctgttctcc ttcagaaaga gggcggcgag cagcaccttg gtcctctctc
      361 cgttggagag cgtgcggaac gggcggagga gcgcgtcgtc cgccacgtcg aggagcgaga
      421 cttcgcgggc gatctcccac tcctccgcgc cgggcgcgac gctccgtatc agctccagcg
      481 tctccatctc ctcgtccgga acatcgtagg ggaagtactc gaagtccacg ctcgacgaga
      541 tggtcccctc gtactcgtac cgtccgagca gcaggttcag gaacgtggtc ttccctctgc
      601 cgttcctgcc ggtgaagccg agcttccagt cggtatcgat ccgaaagcag acgtcctcga
      661 aaatattctc caatccgccc tcgtaggcga aggtaaggtg cgcgacgttg atcaaagaca
      721 tgagcatatc ctcctgtgaa gaaaaaaccg caagaaggcc tttctgattt tccgaatccc
      781 ttcaggacat ccgcccggat ttttagtatc ataaaccgaa acgcttcgaa agaggagacg
      841 attgcacaat gttcacagca tcagaagcat ggaaggcgcg ctatccggac gcctgcttcg
      901 gcgtcctcat tatggagggc gttgacaata cggggaagtg ccccgcgctg gaggagcgaa
      961 aggacgctct ggagcaaagt ctccgcactc tctacgcggg g
//
LOCUS       DAOSWW010001111         1001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Synergistales bacterium isolate AMR_MDS_2127
            NODE_150210_length_1001_cov_3.302326, whole genome shotgun
            sequence.
ACCESSION   DAOSWW010001111 DAOSWW010000000
VERSION     DAOSWW010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864194
            Sequence Read Archive: SRR4236672
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistales bacterium (wastewater metagenome)
  ORGANISM  Synergistales bacterium
            Bacteria; Synergistota; Synergistia; Synergistales.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 05:40:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,166
            CDSs (total)                      :: 3,134
            Genes (coding)                    :: 3,116
            CDSs (with protein)               :: 3,116
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 16 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Synergistales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150210_length_1001_cov_3.302326"
                     /isolate="AMR_MDS_2127"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268201"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..>1001)
                     /locus_tag="PK442_15830"
     CDS             complement(155..>1001)
                     /locus_tag="PK442_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOO89177.1"
                     /translation="PGGLMDVTSRKFVSIAAKYTDATFVVENKQGAGGLVGMQAVLEQ
                     PADGYTVFAATTSNIAKVLTSKKDVNEFIWGFEWIAMLMQDPECLIAAKDNPLNTWEA
                     VHKDAMEKNGAQLWTGPAAGGNDHLLAMKVWDKSGMSAKWIPYSTGPEAMMALLSGQG
                     VVYVGNAADIGGREGFSIIAVARKERLKKFPDAPTFIELGVEGLEEEAMWRGFAMKKG
                     SPEEAMVWWEELLKKVAADPEWKEFLEKDGIDVVDYGREKFNAQVQKDIDNFTVYLKK
                     YGLIK"
BASE COUNT          191 a          329 c          237 g          244 t
ORIGIN      
        1 ccatggaacc acctccgtga gcaccccggc tccattgtta ttgtacgcca ccaagcccgc
       61 cgccgtacta ccggcggatg aagtgcgggg aaccgccgtc tcgggcgata ccccgcactc
      121 gttggtccgt atttgatgcg aaagagtgaa aaacttactt aatcagaccg tacttcttga
      181 ggtagaccgt aaaattatcg atgtctttct gcacctgtgc gttgaacttc tcacggccgt
      241 agtcgacgac gtcgattcca tccttctcga gaaactcttt ccattcgggg tcggcggcaa
      301 ctttcttcag aagctcttcc caccagacca tggcttcttc gggggaccct ttcttcatgg
      361 cgaaaccgcg ccacattgcc tcttcttcaa gaccttctac cccgagttcg ataaaagtcg
      421 gcgcatcggg gaattttttg agccgctctt ttcgtgccac ggcaattatg gaaaagccct
      481 cgcgtccgcc tatgtccgcc gcatttccga catatacgac accctgtccg ctcagcagcg
      541 ccatcatcgc ttccggcccg gtactgtaag gaatccactt cgcgctcatt cccgacttgt
      601 cccacacctt cattgccagg agatggtcgt tacctccggc agccggccct gtccaaagct
      661 gcgcgccgtt cttctccatc gcgtctttgt gcaccgcttc ccacgtgttc agagggttgt
      721 ccttggccgc gataaggcac tccgggtcct gcatgagcat cgcgatccac tcgaatcccc
      781 agataaactc gttgacgtcc tttttggagg tcagcacctt ggcgatattc gacgtcgtcg
      841 ccgcaaacac cgtgtacccg tcggcgggct gctccaaaac cgcctgcatt ccgacgagtc
      901 cgccggcgcc ttgcttgttc tcaacaacaa aggtcgcatc cgtgtacttc gccgcgatag
      961 agacgaattt tctgctcgtt acgtccataa gtccgcccgg a
//
